LOCUS       DAOPWU010000001        86360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_222_length_86360_cov_5.858374, whole genome shotgun sequence.
ACCESSION   DAOPWU010000001 DAOPWU010000000
VERSION     DAOPWU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 86360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 86360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222_length_86360_cov_5.858374"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PKM23_00005"
     CDS             complement(<1..598)
                     /locus_tag="PKM23_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89877.1"
                     /translation="MMSQKIRWWVIAGLVLAMTPARAQITGKIEGISLLEKDLVILGE
                     GRRGPYFLPDSLILSRSERVFLDNQPLTREQYSMNYLKGEIRFNEQVPQMVNIRLQYK
                     INPFPLATVKQRHPLVQRVFGAAGAPGQSLPGESSAGEVDYAAQLNKNGSITRGITVG
                     NNRGLKVNSSLNINVSGKVAENVQVVAALTDQTTPIQPE"
     gene            complement(607..1557)
                     /locus_tag="PKM23_00010"
     CDS             complement(607..1557)
                     /locus_tag="PKM23_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNY89878.1"
                     /translation="MNGTGRTHFPHLTAAALWLLLWGNPAQAGDSLVVRFLYAFGKEG
                     GEPGQFRSPSALSIDPEGALYLADAGNNRILKLSVTGEVLAMTGGFGWGINQFHRPAD
                     LCAENGLDLYVADYENRRIIRCDRDLHWIDAYYYPAGTDDKLNLGFPAGIALSIHNDL
                     FVTDAENLRVLRLNGLREAVYSFGDFSEGEGELAEPQKITIDREDRILVSDRLRSGIV
                     VFDYFGNYLHEIGAGVLKEPAGLQTARNGALFVADTGRDQVLVFSQSGALLAAIGSAG
                     DKLGALANPIDVAVSGDRLFVAEAGNHRIQVFELQWLQIP"
     gene            complement(1631..2170)
                     /locus_tag="PKM23_00015"
     CDS             complement(1631..2170)
                     /locus_tag="PKM23_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89879.1"
                     /translation="MTKNYINLSEVPSLIFIRPNFSLTFALFNFNLANEITVQATLSS
                     ELGMAKAKAKSRGPDRSAVKTTFFFFLILAALFLVARSGLLSGPWDTLVGASPIYDTD
                     ARNDRDIKRNLTRAQLRLLDRILEAELDQGGRRLGDREVRQIARGDKAVMAAYDRWRA
                     YLEMRRSSNYVIYPRDRGR"
     gene            2265..2834
                     /locus_tag="PKM23_00020"
     CDS             2265..2834
                     /locus_tag="PKM23_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNY89880.1"
                     /translation="MPQSGKGGGWWKAARSALRRTALLLLMGQLACAWNPGFRQGVYP
                     GEDVERLAANTRGERFGEMTPETLERFKAEIRTYWQSPYLWGGNAPSGIDCSGLVGRI
                     YQRAAAIDLPRTTLELYEIGMSVQDQPLQFADLVFFSFGGNRQPDHVGLYVARGYFIH
                     ASVKNGVTLSLITEQPYLNRFMGARRLLQ"
     gene            2877..4364
                     /gene="rlmD"
                     /locus_tag="PKM23_00025"
     CDS             2877..4364
                     /gene="rlmD"
                     /locus_tag="PKM23_00025"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNY89881.1"
                     /translation="MDGQNKEPILLKSKPAAGVHRGDLVELRPTDLALGGAAVARLDD
                     LVVFVENALPGQTVLAKIFKKKKNFAEARLEKVLTPAPGQVEPRCAHFGHCGGCRWQH
                     FAYGQQLESKQRQVEETLRHLGGFAGLTVQPTLPSPEIYGYRNKMEFSFSPLRWLPFF
                     EKGREDATAEALYLGLHAKGFFDKIIDVESCHLLPPLSSEILQEVRQFAIESGRPAYH
                     ARDHRGFWRFLVVRHARKTGELMVNLVASEYDRSLAETFTQRMQARFPQISSLLYSTT
                     QSLSGVAFSEAEYLLAGKSTITENLGSLSFEISSNSFFQTNTLQAERLYDVIIDYAQL
                     RPDETVYDLYCGAGTISLYVSHLAERVVGFESVKAAVDDAHHNAAHNRIGNCSFVWGD
                     LRDALEDTGKTIARYGRPDVLIIDPPRGGMHPKTVAAVLRLAPQRIVHVSCNPATLAR
                     DLKLLCAESYRLTKVQPVDMFPHTAHIEVVVLLQRNPSSAQDATV"
     gene            4380..6296
                     /locus_tag="PKM23_00030"
     CDS             4380..6296
                     /locus_tag="PKM23_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNY89882.1"
                     /translation="MNIFKKLFGKTEKKTTPAPRPQSGSDRHDQGRSRPRYQQGQRPG
                     SSSGSRPVGGSPQRPGGQPPRPKPAPSQEPRTSESSESRPQGSASSRSRRRRRPRRPQ
                     DRPAQLQSASQQTTLPLQSPGVTPQPKPEAVETPAAVVEAPVRVPKEPLPEITLEQLS
                     EPIAAGLKRANWEQLMPVQARTIPYLLGHRDLMVQSRTGSGKTGAFILPMLELLDPSR
                     AETQALVLVPTRELAKQVNGDAQLLFGDSGLRSTAVYGGVGYGSQIDSFREGAHLVVG
                     TPGRILDHLLKGTLNLNRLKLLILDEADRMLSMGFYPDMKEVQQYLPKGINAYMFSAT
                     FPPFVLHLADEFLDDPELISLSGDQIYVAETEHSYYIVPPMDKDRALVRIIEMENPAQ
                     AIIFCNTRSQVHYVAIVLQRFGYNADELSSDLPQNAREKVMARLHNGSLRFLVATDVA
                     ARGIDIPQLPVVVMYEPPEDPEAYIHRAGRTGRAGASGEAVSLVTEVEQMDLDRIARK
                     FNFILIPRVLPQDEDVSARVTERVTALLEAKYRAKDNVEKERLRRFQPLAQSLADNSD
                     LNSVIAMLLDEFYQQLLHAPVQTTDSVQAEEQQIERERERESSQPQHRNNRPRRRGGR
                     HRRPGGRPQANPIE"
     gene            6377..7225
                     /gene="nfo"
                     /locus_tag="PKM23_00035"
     CDS             6377..7225
                     /gene="nfo"
                     /locus_tag="PKM23_00035"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNY89883.1"
                     /translation="MKYLGAHVSASGGLENAPLNAHDIGAKAFALFTKNQRQWLAKDL
                     TTIEIDRFKLRCSELGYSPDYIMPHDSYLINLGHPEKEGVEKSRAAFLDEMKRCEELG
                     LTLLNFHPGSHLGQIPLESSLQRVAESINIALGKTRGVSAVIENTAGQGSNLGSRFEE
                     LAEIIAQVDDQSRVGVCLDTCHLFTSGYDLRTAEAYAATMEHFDAVIGFDRLRGVHLN
                     DAKKELGTRVDRHAPIGEGYLGLEAFRLLMQDPRFDDIPLILETPEPERWPDEIRLLY
                     SLAAEA"
     gene            complement(7305..7904)
                     /gene="liaF"
                     /locus_tag="PKM23_00040"
     CDS             complement(7305..7904)
                     /gene="liaF"
                     /locus_tag="PKM23_00040"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HNY89884.1"
                     /translation="MASQSNRGSFIMGLVLIVIGFLLLFDGPSLFDIIRWIFHFWPVG
                     LIALGLYLVMGHRRPGGSGYETPSDIAAAAPPVEGSLYRDSLVGDLRVKLPVQGFNGG
                     VVKTLVGSVVIDASQTVLEPGEHRLYLRSGMGDVHLDLMPGMAVKVQAHVGLGDIKVF
                     DQKAAGFNQEMAYQTPRYDEASARLLVITRVGLGDIKVF"
     gene            complement(7961..8896)
                     /gene="trxB"
                     /locus_tag="PKM23_00045"
     CDS             complement(7961..8896)
                     /gene="trxB"
                     /locus_tag="PKM23_00045"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNY89885.1"
                     /translation="MRDVIIIGSGPAGLTAAIYAGRANLRPLVISGLEQGGQVTLTND
                     LENFPGFPDGVSGFEMYQLFEKQAVRFGAEMLYDVVQSVDLTGEVKIVKTATETFEAR
                     VVIIATGSTPKRLGVPGEDKYIGKGVSYCATCDGFFFSGKQVVVVGGGNSALDEGLFL
                     TRFAEKVTIIHRRDRLRADAILQERALKHEKMAFIWDTVVEEVMGGTAVTHLRLKNVK
                     TGAVSNFPVNGVFVFIGHKPNTELFTGQVRLDETGYIETDHRTHTNVPGVLACGDVQD
                     AIYRQAITAAASGCAAAIETEKYIAENEGRGYPSR"
     gene            complement(8989..9729)
                     /locus_tag="PKM23_00050"
     CDS             complement(8989..9729)
                     /locus_tag="PKM23_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNY89886.1"
                     /translation="MLLRDDVVTYLADYLRVGEIKDYGPVGLQVEGRTQVRRIVTGVS
                     ASLALFEEAFKREADMILVHHGILWDKESRVVQGPFKQRLKALLDHDVSLLAYHLCLD
                     VHEEVGNNALAAKALGMHNVAPLGEIGLKGSIEELDFAELQQLLLYVFDHEPFAFSFG
                     PAKIRHIGYCSGGAAGDLALAIEEGLDAFITGDVREPTMHLAQEAGIHFFAAGHYATE
                     RLGIRALGENLMRKTGVEVEFIDIPNPA"
     gene            10034..11305
                     /gene="hisS"
                     /locus_tag="PKM23_00055"
     CDS             10034..11305
                     /gene="hisS"
                     /locus_tag="PKM23_00055"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNY89887.1"
                     /translation="MEIRYKSVSGTRDVLPEEQPYWRFIEKKIAEITALFGYQRVDPP
                     IFEETPLFIRGVGETTDIVEKEMYSFLDKGKTPLTLRPEFTAGVMRLYLQNGLHMLSK
                     PVKLYSIGPVFRFERPQAGRFRQHTQFNVEALGEQDPAIDLEVMSVAWQLYAELGFSD
                     LAFQLNSTGCPQCRPLYIERLKAYYREHLHEICEDCKRRLERNALRLLDCKVAGCQPV
                     IAAAPVISEHLCPECDNHFSALTSYLRGLGKPYIINHKLVRGLDYYTKTVFEVWAKGI
                     GAQNAMCGGGRYDGLIEQLGGDPTPGIGFGSGIERIILSMKEQGLTPEPLPAPRILVA
                     HAGSAAKTAAIEVVFRLRSHGIGALMAFGERSLKSQMREANRLNAGVVIIIAEHEVEA
                     QTLTVKNLTDGSQEEIPAADLILHFKTLGLH"
     gene            11322..12104
                     /locus_tag="PKM23_00060"
     CDS             11322..12104
                     /locus_tag="PKM23_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNY89888.1"
                     /translation="MSLSRQQIRDLRRLQQKKYREEERAFLIEGVRLCEEALAAGAPV
                     ECAFFFPDGMENPRARSLLQALSGREIPLQPVDATTLAALTETRSPQGIAALVRAGET
                     GPFSPAGNREPLVLAVDDLADPGNLGTLLRTAVWFGVKSLLLSRDTVELHNPKVVRSS
                     MGALFRLRIHEGIDLEQTAAAARSAGWRLLAAEAAGGQPAHRISGGGRDLLIIGSEAH
                     GLKSGLDQLIDLRITIPGSGGVESLNAAIAAGILLYQLTGSH"
     gene            12120..12947
                     /locus_tag="PKM23_00065"
     CDS             12120..12947
                     /locus_tag="PKM23_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNY89889.1"
                     /translation="MEPEVDNSLLDARALSRIMLWTLAMFLFSIFFGAVMVFLAGPKT
                     GLLAETLFIIPALLFVYRRRMPFARTFRFNPVSPQILFYTLLLALPVMILGDELDRLI
                     AIFFPLPSWFDVGDLMSIHSLSDGFLIIGNGVIVAAIAEEMLFRGLIQRSLERMHDIA
                     TAIALSAIIFAMFHFNIWWMIQITLLGLVLGYIAWRSDSIWPAILIHGFNNLLSILAA
                     NAAEEKLAWYTSGEHVHWIWLAASLLLLIPAFAGFQKACTARRLPAATDRTIGDDHD"
     gene            12940..14472
                     /locus_tag="PKM23_00070"
     CDS             12940..14472
                     /locus_tag="PKM23_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNY89890.1"
                     /translation="MIKSMPQVRIGMIQSAETIRFQCTSGFALLDLQDRRIAEGTAGA
                     VQEAVIARAESASLHFRVRLAIALTGEEAHLLQEKLAARNIATFLWRPGVVLELADFV
                     MDNREYWIVTEPFADEAAAKSFAAGYEPVGEAVVVKEIVRKATGTLRVMDQTITDGCR
                     VVPEDPEAHIQLLDVTVGIEFHWQHKRTQLLPGILEICFNNAGRLQAINELDVETYLI
                     SVNSSEMTKENPIELLKAQTIAARSTILATMGKHHYDERFHLCSDDHCQCYHGVANIS
                     EHSRFAAQETEGVNLIHGGRVCDARYAKICGGIMEDYGHIWDNREVPYLVSGVDGREK
                     LEYPLANETQVKAYIDSSPDVWCNTQRYEIPSSLPYNTRELFRWRVSYPRLELGELIS
                     RRLNITFGELVDLIPGDRAPGGRLIHLDVVGSERTVRIGKELVIRRALSESHLYSACF
                     YIERDRDSGGRVTHFHFVGAGWGHGVGLCQVGATVMAQQGFDCQAILSHYYRDSRLVK
                     LY"
     gene            14557..14913
                     /locus_tag="PKM23_00075"
     CDS             14557..14913
                     /locus_tag="PKM23_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY89891.1"
                     /translation="MKIVIYNDELRSGMQMYLALSSRHDVRVAQDVDDLFTMLDQTAA
                     DLTILDLVSPATPDEQVDGFDIAHKIQAQHPQVKLVGIYDQGDQNLVKKARANGIAEL
                     IARPIKNRELLGLIEK"
     gene            15051..16661
                     /gene="nadB"
                     /locus_tag="PKM23_00080"
     CDS             15051..16661
                     /gene="nadB"
                     /locus_tag="PKM23_00080"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNY89892.1"
                     /translation="MSMETDFLVIGSGIGGLSAALKLAKKGTVAIITKKHKSESNTNY
                     AQGGIASVFDKGDTYELHIQDTLTAGAGLCHEDAVRLIVEQGPKRVKELFNIGVPFTR
                     NASGQFDLGREGGHHHNRIVHVKDHTGRDVESALLEAVRQNPQITVFEHHMAVELITE
                     HHVFAARPATDLSINCWGAYVLDINDNRIEQFIARATVLATGGCGQVYLHTTNPSIAT
                     GDGMALAFRAGATIANMEFMQFHPTSLYHPEANSFLISEAVRGFGGRLIHKDGESFME
                     KYHPMAALAPRDIVARAIDTELKKRGVPCVFLDVSHLEPEAVRNRFPNIYENCMRYKI
                     DITKEPIPVVPAAHYACGGVRTDLKGRTSIAGLFACGEVTCTGVHGANRLASNSLLEA
                     VVFAHEASQAAIRFAAKRNRIPTPIPQWDDSGTFNQEEWVLISHDRLEIQNIMWDYVG
                     IVRSNARLERALSRIRLIRQEIENYYKRTRVTEGLLELRNLATVAQLMILSAMLRKES
                     RGLHYTTDYPKRDDDAFLHDTLIDRSGV"
     gene            16694..17830
                     /locus_tag="PKM23_00085"
     CDS             16694..17830
                     /locus_tag="PKM23_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNY89893.1"
                     /translation="MIISKIEIRAFKSIYELTLPLDPHINVLIGANESGKSNILKALE
                     AFRDKVALDASFTCQYSNHYYLAKAPELALEFTGFTKENRRLLQLLSDTFKEVDRFTI
                     RKDGPTLNDYHLFIDGSAVESVDIAKLLRVLPKILYFPDIRLLKNRVDFDDLLAGLPD
                     CATERNLLKIGGVDNYELIFEDNARGRRALEEASRIITEQLRRVWSQEPSIEVKLNVN
                     GRSLYIDFSDSTTVFDTPESRSLGFRWYLSFYVNFLAQTFEAQANEYLFLFDEPGIHL
                     HPAGQKDLMKVLEELALKNQVVFTTHSPFMIDRQFPERVILVVKDKNGTRIDNQAYRD
                     HWKPLREEIGLKVGDLFFFSDSSIIVELPTRKSGLLEKLKGKAE"
     gene            17915..18718
                     /locus_tag="PKM23_00090"
     CDS             17915..18718
                     /locus_tag="PKM23_00090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY89894.1"
                     /translation="MSESTAYKERYWLYQYRLGREYLLPLLAEWGIGLEGRRVLDIGC
                     AEAGILCAVADAGGSSVGVELSPSRLRLARQFASPTHLTRMELIAADFFHMPLKPQAC
                     DFDLILLRDVFEHLPDKAAAFSALVRLMEPETRLILTFPPFYSPFGGHQQMLGGLLRR
                     MPWFHILPGPLWWFFAGHIKKRDPNPRFLLEMEKLRRHRMTIALCKRLARRHGLEIEG
                     QRYYFSRPSYQLRFGWPVIQAQTIGRIPVLRELFITGAVIMMRRKWPAS"
     gene            18703..20025
                     /locus_tag="PKM23_00095"
     CDS             18703..20025
                     /locus_tag="PKM23_00095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY89895.1"
                     /translation="MARVLITTYYFPPAGGAGVQRTLKFVKYLREFGWEPVVLTARDA
                     DYPAFDESLADELPADLKIYRAPLFEPYRLYRRFTGKAAGTATDIATLTRDDKSQQKT
                     SERISEWIRAALFVPDARMTWLYPACALGRKIIREEKIDLLFSSAPPYTTHLIGRKLK
                     RSSGLPWVADFRDSWIGWLSTPQWRPAPARALERRMEREVLTEADRILTVSPGVEEDL
                     LSRHPALRDARWRRLPNGFDAADLEGITPAPRSPGFTLIYTGSMYGNRNPRALLRALE
                     RLQDTRYPGLEGLRVRLVGRVGEPILQEITASPAGRHFEHIPYVTHTSSLQHLLAADA
                     ALLIIDDAPANRGIVTGKVYEYIGARLPILALAPEGDAAALIRDHGLGWCAPPADIEA
                     ITAALRSLLERRQPPGAERKETAWQLFERREQTRQLAGIFNEVVRSAK"
     gene            complement(20077..20658)
                     /locus_tag="PKM23_00100"
     CDS             complement(20077..20658)
                     /locus_tag="PKM23_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY89896.1"
                     /translation="MAKKLNLKVDSSDVRDFQGRVRFKFTRKFVQEGITGNGAVLSIG
                     TENRFDTLMAEQFNLPISFTSGDFDREWSAPEQKYDYVFCFEVIEHLMNPLHFLDGLH
                     RYISQDTRIFLTTPYRPHLFWNNQHFHEYDQLRFKHLLDSAGFAVVRHQTRIAWYHPL
                     FYLSGVRPFLRLTIGRSRTHLFELKKADQQPSL"
     gene            20860..21885
                     /locus_tag="PKM23_00105"
     CDS             20860..21885
                     /locus_tag="PKM23_00105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY89897.1"
                     /translation="MPKTVLFIKPSNSTFILKDETFLQEEYRLFSHHYVYGNALRHLC
                     STIHLFFWLLRRLAAADVVIVWFADYHSWLPVVLARILGKPSIVMVAGYDVARLPEFN
                     YGVFVRRFRGWCARIALRNADHLCPVAASLVEDIRTHIGAVKGRIQVIPFGFSAQKWY
                     PAGEKENNLILTVSVGEDLRRMRIKGLDLFAAAAQRLPEGQFMIIGPQGEMLNHLREL
                     QIPNLQLLPPLPPAELLSHFQRAAVYAQFSIREGLPNAILEGMLCECIPVGVPAGGIP
                     EAIGDAGFMAASRSVETVVEALHQALAADRQLGRKARERTMILFSEERRRSALREIIA
                     GAAPHAN"
     gene            21980..24526
                     /locus_tag="PKM23_00110"
     CDS             21980..24526
                     /locus_tag="PKM23_00110"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNY89898.1"
                     /translation="MKKKKVAKEKTLAPLQPEKETIISRHPLAVLLVIAFLLLLIFYS
                     QVMLAGKTFQMPDQQSSRATAPFVKEALARGITPLWCPYIFSGMPSFGSLLSAPHVNR
                     LDEFFTGLFRTAMMPEFTFIFFNYLLLALFMFLLMRGLAVEPLPALFSGLAVIFIPQF
                     IAFTAYGHNTKFLAVVLIPVILLLAKKMMEEKNALWFSLTALAIGFQMVRAHIQVSYY
                     TFLLLGIYFLYKEIADFRRSGEFKPLLISAGLLAGAVFAGVLLSMTLYTSVLDYQRYS
                     IRGGSGEGGGLEFDYAANWSFHPLEMVTFFIPSFMGFGGATYWGKMPFTDYPLYFSVI
                     VFLLAGLAILLRRDRTTWIMTIVALFSLLVSFGKHLPLLYGPLFKLLPYFNKFRVPSM
                     IHIILDIAMLILAGIGLQALFELRTQLPQLKPMERSRKMKGVKRYLYAFTGIVLLLVL
                     IVLLGKSIYMGMAGSGGHTLNEAQRLEAYNKALLDSFKSIGLLALAIVLIFQFLKNNL
                     TRLTLTLSLSAMVIFDLIMVDAKIIHPRDKSDEKAFFAATPAVQYLKQEKELYRIFPV
                     LDDKSGNWYMYHLIPNISGYSAAKVRLYQDFLDETGFGSQDRYGLNSFMSKYWRVGMR
                     DNRVTPVEVPLDQIAPERRAFDSAMLDMLNVKYLVLQYLPLNDPRYQPVADPEQIPLY
                     RNTTVLPRAFFADSTRQFTGRKNIFDFMKSGRFDPRRVAIIEENPPFAVAPAGANTAE
                     ITRHDLHQIDIAAQVKSPTILVLSEIYYPAGWKAYVDGKETRIFKTNYLLRGLFLRPG
                     DHQIVFKFDPASYRAGKWISTLTLLLLLGLLGYALWLLYQRRRKQPPVTA"
     gene            24577..25770
                     /locus_tag="PKM23_00115"
     CDS             24577..25770
                     /locus_tag="PKM23_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNY89899.1"
                     /translation="MSFRKTLYMFSGTLRSLFVLVVILSIAAVNFYVQGLVRDLRKQS
                     RDIAEFYALTYQRLAVAADQPEVLDWLFVNIISRATFPLILTDRDGNPSSWQGLAIPD
                     TARSPEAIRQVRKVLEKMRQENEPIPITYQEYVLSYLYFGDSMLITRLQYLPWVTISG
                     LGLLVLVAFLSFRSIKSSEQRFIWVGMAKETAHQLGTPISSLMGWLELLKSSNHEGPN
                     REQTLTDMEADIKRLEKIAARFSHIGSETLLDPQDLQPILHDVCAYLRKRLPKSGNPI
                     ELIENYSELKPVPLNRELFEWAIENLVKNAIDAVKGKRGRIEITTTLLTGKKRVAIDI
                     SDNGAGITAKRRNDVFKAGYSTKKRGWGLGLNFAKRIIEEYHHGRLFIKETHIGKGTT
                     MRIII"
     gene            25767..26522
                     /locus_tag="PKM23_00120"
     CDS             25767..26522
                     /locus_tag="PKM23_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNY89900.1"
                     /translation="MSGTERRLEGRVALVTGSARGLGRGMACALADRGAALAIHYRQS
                     NREAARLVEELHAQGVEADLFKADLRRSEEAGRLQEEVLVRFGRLDILVNNVGPFIEK
                     PWNELKPAEWEEMVEGNLNSVYYLCRAFWPVMVAQHWGRIINIGLANSDRIQAYRQVL
                     PYAIAKTGVLILSKSLAVEGAPHHITVNVLAPGLMDNGTLNASGRRAAAARIPAGRPG
                     TAEDLAGALLYLCSEEAAYVTGAHIPVSGGWGL"
     gene            complement(26519..27286)
                     /locus_tag="PKM23_00125"
     CDS             complement(26519..27286)
                     /locus_tag="PKM23_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89901.1"
                     /translation="MNKILPADTNTVLLLLLVVSTIAAFMMLGFGGSAGRKKKAPARK
                     KSRTPSKSLLLMLLLAASAMVLLFFAVYLHYYTPLKGETRVARVEFEKEGGSKGDFSL
                     ILIPYENGQPGKAQSYPIKGAAWSLKGEVLHWKPALGKVGLRTMFRLTAIDGHYGQGG
                     SARKGSEVKLTGTAAEGIWKAVQGANRIFKAARITTHRSGRVDPLWSGGYDVYADEEG
                     VRIESAGKTPAALKRESREAAPHQERRYPRRPEDVRH"
     gene            complement(27294..28001)
                     /locus_tag="PKM23_00130"
     CDS             complement(27294..28001)
                     /locus_tag="PKM23_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89902.1"
                     /translation="MKALLPAFILLLACDHGPDVNEFNYEPEVNLFALLLLNSQQTII
                     RLEETSRSMDPLPDDRGIAGAELSIQGGEQRVAYRHQGGGIYAEEGEKLRLIPGVTYE
                     LLALLPDGRRVEAECTMPVPPDIIAPAEQERVEAYASLPISWRGEQGTPGYIVSVRGI
                     TSGHNAETKTDSTAVDFFPFYLAQPDRYVLKVAAVDRNYNEYLRVQEDEVPIWRVRGG
                     IGVFGAMAWDEHVIIAQ"
     gene            complement(28006..30279)
                     /locus_tag="PKM23_00135"
     CDS             complement(28006..30279)
                     /locus_tag="PKM23_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY89903.1"
                     /translation="MKAPPVKPPVLLLVLQLLLGVGGVQAGAEVKPASISGFVRDATN
                     SEPLPYANVYLDGTRFGSLTSLRGYFVIPHLEEGRYLFTVSLLGYERWQRELRLRAGQ
                     DTVLAIALTPVAIQMGEVTRTAQRERFEREVQISTTALSMRQLQQVPTLAEADVFRTL
                     QLLPGVVSRSDFSSQLYVRGGSPDQNLVLLDGVSVYNPFHLGGVFSTFNVDAIKELEF
                     LSGGFPVEYGGRLSSVLSITNREGDARSYGGVASISLLSARAMVEGPIPAGSFLIAGR
                     RTYFDQVFKKSDYAFPYYFYDFQGKINLDLSHRHRLTFSGFYGDDVLDFSSEAEDEDE
                     LDVDLDWVWGNRTTSLNWRYIIRPDLFAETLLSHSRFKNRLSAVVSSSGSANLALNNE
                     IDDYSARTDFNYFGLSGHTIKLGGAYSDLDFTYGISINDYQLFSYSSRPALFALYAQD
                     HWQLYPRLMLKAGARAEHYSLGDRWFLSPRLGVQYQLTSNLALKASFGHYTQYLTTVA
                     SGEQNFAFMDLWLPINASYDPQTARHYVAGLEWWLPHELIFTFEGYYKTFSGLLDLNE
                     QGIASDPTDDYFVAEGTARGVELMVKRQTGNLQGWLGYAWSRTRRQSDGIFYYPKYDQ
                     RHAFHLVLNYTLGRGWTLGTVFSYGSGMPYTPMPGKYMHYEWRLDENALKGELTDRPG
                     GRNSARYPLYHRMDLSLRKQGRLFGISTSPYLQIINLYNHKNVFFYFWDHDVNPSRLT
                     TVTMFPLLPTVGVDFAF"
     gene            complement(30297..31076)
                     /locus_tag="PKM23_00140"
     CDS             complement(30297..31076)
                     /locus_tag="PKM23_00140"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNY89904.1"
                     /translation="MKNWNKTAWALAMLCGALFTLHGQSYTQAANPEELRLFPHQEFQ
                     AGEYLEYRVHFGKMSAGRGSLWLKDGEEYNGRSTYLLTMRLWTNDFFSSFYRVDDVMT
                     SILDREGLFPWGYERILREGKFKAFRKARFDPLNARAFEGKDTLAVPSYSQDVLSIIY
                     YVRTLDLKVGQKIDLDSYIDKNVYPLTMTVEKQEKIEVPAGKFDCLLLVPGKRPGSTE
                     ELKGEMWIWLSMDDRCLPVRIKSRAPLGSVRMELDKVKIEN"
     gene            complement(31073..32161)
                     /locus_tag="PKM23_00145"
     CDS             complement(31073..32161)
                     /locus_tag="PKM23_00145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNY89905.1"
                     /translation="MTSTGKKTPGYTPERILVIRTDRIGDVVLSTPVLTALRQAFPSA
                     FIAMMVRPYTRELVSGHPHADEILLDDGSGEHAGLAGFLRLLRLVRSRRFDTVLLLHP
                     SLRLALLCLLAHIRHRTGTGYRAYSFLFNHRLFHHRRKSGRHELDLNLDLARALGAPL
                     DHVGFYLHIPEEAHSAVASRLEELGISPGSGFVVLHPGSGGSAMDWPLSGFAILADRI
                     QEELSLTIVITGNKAEAPLVDRLCSMMQSKPRRLDGALSIKELLALLSRAHLLVANST
                     GPLHMAVAVGCPVVGLYCPLAACAPVRWGPYGHLDSVLTPPLPACGKCTGERCAHSNC
                     MELITSDRVFAKVKEQLLNSAVIPPGKI"
     gene            complement(32158..32796)
                     /locus_tag="PKM23_00150"
     CDS             complement(32158..32796)
                     /locus_tag="PKM23_00150"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNY89906.1"
                     /translation="MSETIKINSKQPEDDLISRAVRILRKDGVIGYPTETVYGIGSNI
                     FSAAAVDRIYNLKSRDRSKALIVIAADIIQVGDLVEEIPEAAERLMENFWPGPLTMVF
                     RASRELVGSPFHRSHTIAVRIPDCPICLALLKSSGFPIISTSANRSNEPPAVTAEQVN
                     EKFGDELDLIIDGSETLSTTPSTVVDVTRLPVRVLREGAISRLEIDTVLESD"
     gene            complement(32774..33829)
                     /gene="waaF"
                     /locus_tag="PKM23_00155"
     CDS             complement(32774..33829)
                     /gene="waaF"
                     /locus_tag="PKM23_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNY89907.1"
                     /translation="MHKIEKILIIQTAFIGDVVLVSPLIRASHDLFPQASIDALVIPS
                     AANSLENNPALHRLTLYDKRGRDAGVRSLLALAGRLRRERYDLVLVPHRSLRSAFLAW
                     WTRAALRVGFTTSSAAFLFTHRVVYDRKRHEVERNLALLGALGQEPGLVPPEIFPDEA
                     DHRAVDRMLASLGGTTGALIAIAPGSIWATKRWPAESYRRLAEMVLASGWRLVWIGSR
                     GDRTLCTEISEGLTGFWVNSAGELSLRQSAELIGRAEVLVSNDSAPLHLASAMGTAAV
                     ALFGPTIPKFGFGPLSAGSVIVQIELPCRPCSIHGGNSCPIGTHKCMTGIPPEMVFAE
                     LSNLIMRKSKHERNHQN"
     gene            complement(33816..34739)
                     /locus_tag="PKM23_00160"
     CDS             complement(33816..34739)
                     /locus_tag="PKM23_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNY89908.1"
                     /translation="MKQIQNVVEYLLVQLLVFPVRLMPHRLALRLGVRLGGFVYHIVP
                     IRREVALQNIARSFPEKSDDEVRAICRQVYRNFGQNIVEFIRMPKMDDRFYREYISVV
                     GGELLERAGHEGRGAICLSGHFGNWEFFPAVIHRLGFPMVGLARDQRNPLVNKLINQT
                     RTGWGVEITHLGMGVRTIFRALKANKFVTILADQDAHNEGVFVDFLNRPSSTASGPAL
                     FALKTGAPLIFGVVVRGQAGRHTLQLEEIDHRDLQGVTPENIHELTQRHARLLENYIR
                     LHPDHWLWMHKRWKTSPPAGPAEDGNPVAQD"
     gene            complement(34770..35831)
                     /locus_tag="PKM23_00165"
     CDS             complement(34770..35831)
                     /locus_tag="PKM23_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNY89909.1"
                     /translation="MRVPLALLIITLLLAFSFAATASTEGVSYLRHCSGTLCIYYTIE
                     DSRLLEETRSILERAAEEIAHDLQLVPRDTLRVMIVPTRESFRDYLRGHMPEWTQAFA
                     VPATSTMMVRSPRWDRPESSYSQSLVHELLHLLLHQRLGNRELPRWLDEGLAIFYSEH
                     EQYEKRSRISRALATGSLIPLQEIDEVLNFHRDRAQLAYEQSYSAVRYLLATYDIEAL
                     RAILDGYAAGESSDAILMRATGSTMSGFEEEWRGHLEKTEKWLWLSEVDELIWLALPL
                     LFLIVVLLVRRRNRRQLREWESARQLGEGDVLRLPAEGEGGYETPWVAPHLMPRRDEE
                     DDEEEPPEGEPGGEDGIRS"
     gene            complement(35828..36202)
                     /locus_tag="PKM23_00170"
     CDS             complement(35828..36202)
                     /locus_tag="PKM23_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89910.1"
                     /translation="MIMALPYSTKLDLLRALLCGGGLQEQLEALPPAGLLEAHRFVWE
                     KTVEFGIKMRGRRFPQEELQMALRARGDLRAAKGCPAQLRQCRLQECAEHHPECMREA
                     AMEQILAMGAAVSEYMHRPDAL"
     gene            complement(36245..36709)
                     /locus_tag="PKM23_00175"
     CDS             complement(36245..36709)
                     /locus_tag="PKM23_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89911.1"
                     /translation="MAEIVVVGPEGDGKGAFLHSFCPILHETEAGILLGEMPINRELV
                     LYCYGIGYDRSFAWDLVGRKMLGYLLCFNWFDEEAYAACREMVTFASSSFAAPFVIAA
                     DHGLQPLPVPESAVRPHIALSSSARFLFYQGHKPASVRKVVTTLLDLLLEKL"
     gene            complement(36935..38020)
                     /locus_tag="PKM23_00180"
     CDS             complement(36935..38020)
                     /locus_tag="PKM23_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNY89912.1"
                     /translation="MSGPNTPRQNILIVDAASAATTQLVNALHDHHLESIVIAESAAA
                     LEHAFAAPPLLILSELEQPGIDGADLFRRIRAHADTQDTPFIFITRLPELETRLKLLS
                     LEIDDFIAKPYYPEEVVARVESILRESAVTPGGDVPLNQGFTGSLEEMSLMDLIQTLE
                     LGKKSAMIHLVQEPDEGYLFVDQGEVVDAVLRDSTAEEALDNLMLWLHGYFELTMQPV
                     QRERRIIRSNRELLLAGAQRLHEYRQRSAQLPSVESYVARAADVVRPELPEREARIWE
                     LLERPQPIRLLVASLGEDELRTIEVIQALWERKLIVISPGKASDADLIASSMIRQVSE
                     SRQRSAEPYSRMASFFTRNGHQKKNLS"
     gene            complement(38036..40069)
                     /locus_tag="PKM23_00185"
     CDS             complement(38036..40069)
                     /locus_tag="PKM23_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY89913.1"
                     /translation="MNPLSILLIDPDPKNVRILRNNFLDLQFDVDTAANDSEALEKIT
                     ARSFDAILSEVSAPGIDGYALLEQAQQSPLNRSCSVIFLTQKSDVWNRVKSFKLGAKD
                     YIIKPIHVREIVMRVQMVLKRQERYRQLLKRSRSQFSGRLADLGAVELIEVFGAERKS
                     GVLTLNNENGVSGRIVFKEGAVIRASAGLQRSEEAVLKMLSWKKGRFTMVFAPVDGTD
                     EIGVSNMGLLLQGAKRMEQREELLHRLPALETVLVTTQNFKKIIAKKELASDLEYFVR
                     LFDGAHSLGRIIDESKYDEITTLNRILKLYELGFLHSLRDFEPADELGETSRAVEQAP
                     EMAGQAGVLPSSGLEHVEKAIGSETTEELLHEQIDGSSPEAEPLPAGEETPLFVEEPG
                     VLQPLAPDSSAWNSDSSFDKDLFYDLRRAEEEGQDLFAGSRSLEGGSVFSDLDRVIPH
                     HPADALLEIGAEEELAEAPPSEPVPEEEEGADEVRELEQHVRIQEKFRSAHGSILILG
                     NNRELCRQMIANLAIDAVQEKSALQPADSDLILGTAEFKGGHLLNLIALSLEREFAPV
                     IDFFAGSLLGFIFLIDGRSVDWGYQRYLRQVLREKTSLPAIIVFSYNEYLKKPLEVQT
                     IRQRLGLRERDGLQLVSDFSVVNSRRIIFRLFETFYRPQARRDARTKTIQTTS"
     gene            complement(40230..40874)
                     /locus_tag="PKM23_00190"
     CDS             complement(40230..40874)
                     /locus_tag="PKM23_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89914.1"
                     /translation="MSERHRKVDCPGTRELELYCLDELTAAETREQIKVHVKSCPRCA
                     RKMQNLYAFYAILSEELERPVRPGLLDFCKKRACNKVKYGLLICRPIPEKDRQRGKAY
                     LATLAFSANGDGSKTSLTDFNLSRDQIGVMLYSDPLHNEVSLFLWSKAEGGSAPCRLE
                     APGLFEKTDFNLAGAAKIPLTNFEYLNNRLLYFSMKKRAAGRPKLVNRVQEMLL"
     gene            complement(40974..42029)
                     /locus_tag="PKM23_00195"
     CDS             complement(40974..42029)
                     /locus_tag="PKM23_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89915.1"
                     /translation="MYSICDGSYSQSGLIQYINLAQKISLSYLKYQEMLGKRISGDAW
                     EADLELADLAMDCIAELFGRDESGRFPQLKKYYEGKFAEMPFINDAEVLILTRRLIVR
                     KTKQELSRIFRERDPEGAKIVRNIKVAIRTAADLHLFRELGKEYVFHGPVPKNEQGGV
                     PSARLALYLRRTLPPIPEEMLHKLFLDNYRSGDSVSVSIRRLLEAIHSLADNQNFCAL
                     DAVVKLVRYVKFDVCRERLAAGESVSTPSDELESKEMEEYISVVMESIWSRLDFQYLR
                     THKLPAKKAAIYHRALRDVLYDLIQKRDTSSYYRNLKFYMPELTQKAYRQQERSVFEY
                     LAKLAKKEFRKYLCEML"
     gene            complement(42434..43822)
                     /locus_tag="PKM23_00200"
     CDS             complement(42434..43822)
                     /locus_tag="PKM23_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY89916.1"
                     /translation="MVDETTSKANILIVDDDKNICKMIEASLRKEKRYEIETALSGEA
                     CLRLIKENPPDLVLLDIQMPGIDGIETLHRIREENPYIPVIMMTAHGTIERAVSSMKA
                     GAYDFLTKPFARDRLLVTVNNALMNSSLRREVDELRSELKSKYAFANIIGQSGVMQDV
                     FRAVEKVFNSNVTVLIQGESGTGKELIARAIHYHSTMRAGKPFVAVNCSALPESLLES
                     ELFGHEKGSFTGAVGRRIGKFEQANGGTIFLDEIGLMTPATQSKLLRVLQEREFERVG
                     GNELVKVDVRIISATNKDLEDEMKKGEFREDLYYRISVFPIKLPPLRERREDIPLLAG
                     HFLQKYAKQEGKSIEGITPDALDLMMAYHWPGNVRELENAMERAMVLASGPEIGAKDL
                     PAAVRSLGEKRIYESDNTLASWIEKLEEEALRQALLECEGNISQTAKKLGIGRATIYR
                     KAKKYGLPMVKS"
     gene            complement(43935..44975)
                     /gene="lpxK"
                     /locus_tag="PKM23_00205"
     CDS             complement(43935..44975)
                     /gene="lpxK"
                     /locus_tag="PKM23_00205"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNY89917.1"
                     /translation="MGYFETRWPALLMRPLSGLYGLAIRRRNRRFDSGAAEVFRLERP
                     VISVGNIVVGGTGKTPTVLYLVRWLQQQGQRVCILSRGYRRRSRGMVVVADGEGIRSG
                     VEEAGDEPMLLAQQLPGAVVVVDSDRARAGRHALQHFRPDLFLLDDGFQHRRLHRDLD
                     IVTFKEQSDLGNGWLLPAGPLREPLEALRRADLLWFNESARLHDDLLIHSPADLPSIR
                     ARLGVTDCRNRAGERLDPTPDLRVVLFCGLAKPQGVLNTAREMGFEVVRFICFRDHHD
                     YGQRDIRDLERVREHHRADVIITTDKDWVKLLPAFNLPPHWFHLSVAVEPEDGPHAEK
                     LLLSLCRSHGIL"
     gene            complement(44962..45618)
                     /locus_tag="PKM23_00210"
     CDS             complement(44962..45618)
                     /locus_tag="PKM23_00210"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNY89918.1"
                     /translation="MDKKNKEKVLFFLATRFAWLLILFFGKSARISVRNRKYWNQLVA
                     EGKGFIAVPWHGKMVAPLYLHRHLGVVAMVSEHRDGEMIARTVQRLGYFTVRGSSTRG
                     GRQAFRDMLKALRLNRICCILPDGPTGPRHHFKTGALVLAMRAGVPLLPMTFAASRPI
                     TLNSWDRMTLWWPLSRVRLIYGEPIRVPERLDSEELELFRREVEGRLAKLEEEANGLF
                     "
     gene            complement(45670..46821)
                     /gene="lpxB"
                     /locus_tag="PKM23_00215"
     CDS             complement(45670..46821)
                     /gene="lpxB"
                     /locus_tag="PKM23_00215"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNY89919.1"
                     /translation="MPLKVMLIAGEASGDLHGGKLVAALRGLEPELELFGVGGDRMAA
                     AGMELYYHVNDLAYIGFIEVARHYLYFRRVFNRLIEVVKSRRPEVVVLIDYPGFNLRF
                     AKAVKKLGISTFYYIAPQVWAWGQGRAAKMAGFVDQMAVLFAFEVDFFSRYQIKTTFV
                     GHPLLEGMEVGLSRYEFMEKYDLNPGHPLLALIPGSRNQEVRHLLPPMLQTAEQLRRN
                     HPELQVAISQAASVEPQQFESLVAAAPWARAVRADYYPLMHYATAGMVASGTATLEAA
                     CADLPFALLYRVSPLSFAIGKQLVKIPNIGLVNIVAGEAVVPEFLQDDLVPDQMAPVV
                     ERLLFDEPARSAMQVKLAGVRNRLGEAGASQKTARLVLEQIGLHQDETN"
     gene            complement(46829..47401)
                     /locus_tag="PKM23_00220"
     CDS             complement(46829..47401)
                     /locus_tag="PKM23_00220"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNY89920.1"
                     /translation="MTVRDFFFKYRSYTPIPLIIAALVLARTTPASFAAGLLIALIGE
                     SIRLWSVRYAGSATRTTGEVGADVLVTDGPYGHLRNPLYPGNFLLSSGILIMAWPWMP
                     WLMVIFLLLFYVQYRAIISLEEEFLRNKFGAQYGEYEENVPSVFPRLTSWGRGERVPT
                     TLKKALRTERNSLQSFTAVTILILLRWLVR"
     gene            complement(47447..48430)
                     /locus_tag="PKM23_00225"
     CDS             complement(47447..48430)
                     /locus_tag="PKM23_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY89921.1"
                     /translation="MERVRIGIAGVGKLGTFHCNTLSQLAEAELVGLYDIDDARARES
                     AERFRCMAFSTYAEMLEQVDAVGIAVPTTLHHQYTLPALKAGKAVFVEKPIAATIGEA
                     EEMAALAAENKAVLQVGHIERFNPAIRALEHLLPDPMFIESHRMAPFDPRGTDVAVVL
                     DLMIHDIDIILSLVKSEVSRIDASGVAVVSDEVDIANARLQFANGCVANVTASRISQR
                     KMRKMRIFQRDAYISIDFLQRLSEVFRIVGAEEGEPTTVVLGQIDKGKRKRHIIYEQP
                     KAPEGDAMQAEWQAFLKAVRSRTRPIVSGDDGLRALQVAEEITGIIARNTR"
     gene            complement(48464..49468)
                     /locus_tag="PKM23_00230"
     CDS             complement(48464..49468)
                     /locus_tag="PKM23_00230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY89922.1"
                     /translation="MNAIMKTVPPDFSLVIPSYNEEESLQELFDRITAVMAGMKKSYE
                     IIFVDDGSTDGSGRLIEELHRKDERVKLIQFMHNYGKSAALAAGFRVARGTFVVTMDS
                     DLQDDPGEIPQLLAKLETGYDLVSGWKKVRHDPFIKRWTSKIYNYFTSRFSGIQLHDF
                     NCGLKIYRREVVKTMQVYGELHRYLPVIAHRNGFRVTEIPVTHHARKYGSSKFGIARF
                     ARGAFDLMTISFLTRYKMRPLHLFGVLGFLFFLGGFVITILLTWQRIFENRFLSNRPL
                     LFLGVLLIIVGIQFFSIGLLGEMITSLRKESDYFLVRRCLGCQDIFPQEAADEAHFTA
                     "
     gene            complement(49512..50303)
                     /locus_tag="PKM23_00235"
     CDS             complement(49512..50303)
                     /locus_tag="PKM23_00235"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNY89923.1"
                     /translation="MFHSILTRGASALLLAVIMASGGCATFRRPALLPADTASPQRVL
                     TALESNYARIDHFNGRGRLQLSSPSRSLSLDIDVLIDQPDTLFLRLEALLGLDVGWLF
                     SDRHEYRFYLPTENLYGQGAMDSLRWERFFRIAPGYDLLLGTLVGLERGGALAEPVLG
                     QEEKHLVLTGTTSMGLHTLWIDPGRGVVTRSELCDSTGRLLVRQRFERFSRIKGVQVP
                     HTIRIERPAEKQHLILQYDQISINKKYSAQEIKTKIPASAHRIKW"
     gene            complement(50328..52334)
                     /locus_tag="PKM23_00240"
     CDS             complement(50328..52334)
                     /locus_tag="PKM23_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY89924.1"
                     /translation="MKPLFHTLLCLTLIGMGGCAGSRKAAVAPPVQRHYNREAMRHII
                     NGTIEDLLGEPKNALVEYHQAAEIDSSSAGIYLALAENYYLLEEYNSSIRMAKKALQR
                     DAKSIDALELLAANLEKQREFGDAAEVYEQIITLAPDDMESLYSLTTLQIISKSYDKA
                     LLSYRRLVEAGLADPEYRLRIGHLFFQHRALDQARAIYQDVQRSDPDFEASYLAMAAL
                     SKVAKDTSGAIRIYREALTRQNRFEEVKSELRLLYERSNRFEEAVELFRELVARDSTH
                     LGDKVQLGQYLFQKRDTLAAAQWFEKIIRDHPQSERGYLALGALRRAQRDTLSAVAVY
                     ETAVKINPLFLDSRRRLRDLYAAQNRWEDAVALYESLTNNDSTFVGARIEIANLLAQK
                     GDTLKAIALCEELEKTHGEDWRVPLTLGRLFMVRDENIKARLRFDKALSLRKDLSLLW
                     ILSGVNLAQMDSLAAAEEQLKTAVELFPDDPEINYYLGFVCNRLGNKACARHHLERSL
                     EVDGNNVQTMLALAALYDEEKEHERAEKIYATLVKNESESAIVLNNYAYHLAERNLRL
                     DEARALVERALVLEGENGAYWDTLGWILFRQGDASGARARIEKSIALSPKSAEVWEHY
                     GDVLLAMGEKEPARRAYERALELEPERQQVKAKLLKLIPQEEKN"
     gene            complement(52414..53319)
                     /locus_tag="PKM23_00245"
     CDS             complement(52414..53319)
                     /locus_tag="PKM23_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNY89925.1"
                     /translation="MARQAVRWQAPVILGAGLISISLSSVLIKLCPAPPFVISSYRLL
                     IAALVYLAIARLRRKPVWHAFTTTSRRWALLSGLFLGIHFLAWISSLRYTSVAISVVL
                     VQTFPVFVVLGSWLFLRERPTRLAMTGMVVALGGSAAISLGAGGGGESGMTGNVLALI
                     GALGAAGYYLIGRKLRTDIDTVRYVGFVYSVAAAVALTVTLSSGQPMTGFDLRTWLLL
                     FAIALLPQVIGHTSLNWALKHYSATTVSIFTLAEPIGATLLAFIILRENISRATIWGG
                     LVILAGVALTLAGERRSSSGAKLPE"
     gene            complement(53323..54264)
                     /gene="rnz"
                     /locus_tag="PKM23_00250"
     CDS             complement(53323..54264)
                     /gene="rnz"
                     /locus_tag="PKM23_00250"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNY89926.1"
                     /translation="MADALRLFFLGTGAAVPSLRRALPTLAIAFPAETILCDCSEGSQ
                     QRLMSAGLSAARIRHILISHLHGDHIFGLPGLLTTQHLLDRREPMHIWGPVGLRLYLE
                     GIASATGHRTRFEVHIHEWEEDETGEVQLERFSFSARRLEHSKPCYGFRICAHPRPGR
                     FDSGEAERLDIPEGPLRRRLLDGESIALADGRRISPNQVVGRSRAGSCIAYCTDTRPC
                     ASGVELARGADVLLHDSTFGAELQSLAEETGHSTAAEAAMVARAAGVKRLFLWHLSGR
                     IGEAEDQALLEQARALFPESHLPEDMTSWSLPLVREE"
     gene            complement(54280..54912)
                     /gene="plsY"
                     /locus_tag="PKM23_00255"
     CDS             complement(54280..54912)
                     /gene="plsY"
                     /locus_tag="PKM23_00255"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNY89927.1"
                     /translation="MFTALIVLVLAYLLGSIPTSIIVGKVLRGIDIREHGSGNAGGTN
                     TFRVLGWKPGLFVTLVDVFKGFVAAYWLPQWIAPQLADPHLLQLGAGIAAVFGHIWTV
                     FAGFRGGKGVGTAAGMMLALFPEAVLYCLLIFGAILLATRIVSLSSISAAIAFPLILT
                     IFRYVLGREVPLPFYLFSFFAAALIVYTHRTNVRRLLNGTENRFGQPKKK"
     gene            complement(54940..56754)
                     /locus_tag="PKM23_00260"
     CDS             complement(54940..56754)
                     /locus_tag="PKM23_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNY89928.1"
                     /translation="MKIKIRYIDGARLKRAIIASSARIGEMQDALNNMNVFPVPDADT
                     GTNMALTMQSIVDSARECPFTSLAEVCHCISEGALSGARGNSGAILAQFFQGLAEGAR
                     GRARLSPEEFAQAVAVAAARAREALSNPREGTILTVMQDWSAFLKEQALGEEDFVRLF
                     RDGLARAHESLADTPNKLQALRKAGVVDAGAQGFVHLLEGLSDFIDSGKIAAYRAGSH
                     MVEKLRHFHFHKMDDEIRFRYCTECLVQGEKLDRHRLQAELLPLGDSLIVVGGGHKVR
                     IHIHTNEPDAVFTLAASHGTLLQTKAEDMQKQYDEAARLAPKRPIALVTDSTCDMPAE
                     WIEKYDIHVVPVMINVGERSFRDREEISAHDFYKILERGGERLTTSQPPVGFYTSLYE
                     KLAEGYESILSIHISEALSGTIQGAYNGARQLAGKLKIEVVNSRTTSAALGLIVHEAA
                     RMIEAGLPLAEIKEKLAGVISRSRIFVAIPTLKYLVRSGRLRKTQGFFGTMLQIKPLI
                     SIDAEGRIVEAGKVIGRKRLAERSVELALAHARRLINPRFIVAHMLAPELAVSLRRIL
                     QQEFAEADIPILEASPALGLHVGVGAIAIAIMGDPLPE"
     gene            complement(56793..58205)
                     /locus_tag="PKM23_00265"
     CDS             complement(56793..58205)
                     /locus_tag="PKM23_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89929.1"
                     /translation="MAEAQPPADLLNFRRFLQKNLGGLFAPGRPIFIARAPGRLDLLG
                     GATALAGMPVLHYPLAQAVIVAVQPRLDRRILIRNLNLSRERVLTVEYRLDDLLEASG
                     QPDWRMLRSLSAAQEKSWTGNVLAALALLYPVLEGKSRDFGVNIAIESALPAGAGLGY
                     TGAQLTALLLALQEAYALPPGRVDLAGWGFRIEQEIMRRKCGPSDFQSYLLGREGQLA
                     LLQGEPPEISTFLAFPQNLQLLAVDTGVRQSAEWLKLNETCVSLLIGRTLLSEALSGK
                     NSLRPEAGDSTPVSRELWQRELKKLIPYRFSGAEFLLNHPGAREGEAEIDPEKRYMPR
                     TLLEFAIEEAERVQAFVRLLQEGGPVLDEARCSEAGALLAASHEQFSRISGMVSDDAG
                     WLAAAAAGMGTPAGIYGARVMPWGAAGSVVILAKAGSTDHVHNLVSRLRSQFGRSPLL
                     LAGTSSGAITTGVLSTHFTS"
     gene            complement(58316..59608)
                     /locus_tag="PKM23_00270"
     CDS             complement(58316..59608)
                     /locus_tag="PKM23_00270"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012597253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNY89930.1"
                     /translation="MQRVLAIILGGGRGTRLYPLTKMRAKPAVPLAGQYRLIDIPVSN
                     CINSGISCIFVLTQFNSASLNRHITQTYHFSPLSEGFVEILAAQQTPEHSLWFQGTAD
                     AVRQYVSTFREHKVDEYLILSGDHLYSMDYRPFLEWHHRSGADITISVVPISMAQAGD
                     FGILRVDDDGKVVEFREKPKGEEGLKGMAMSGRASGPWREIPAERPYLASMGVYLFRK
                     EVLISLLRDKSNFADFSKEIIPSSVNELKISAYPFNGYWEDIGTIESFYRANMQLLTQ
                     PRPSFDFFNAEFPIYTRPRFLPPSSVAQSTIVQSLICEGSHIAESSIRHSIIGIRSRI
                     ASGCSIEDCLVMGADFYQTEEERREESARGIPPIGIGANCQLRRAIIDKNARIGANVR
                     ILNRAGVSDAEREAEGIWISKGITIVMKNAVIHDGTEI"
     gene            59873..61456
                     /locus_tag="PKM23_00275"
     CDS             59873..61456
                     /locus_tag="PKM23_00275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY89931.1"
                     /translation="MTTKLSILFYAYPQLPYETESFEKMLASLQNQYDHNFEVLLFGD
                     NDQVRKRAEKAGYIFIPTDLDHKAKALNSALRQARGEYVLICNNAANALEFKRSATRF
                     YAMAMDRHPETSMLYTDYDLIDKGVRKERKLLEWHNGRLTEAWDTGYCLLFSRAFLER
                     VGFCDEHYRFNPLNDLRFKAMETGRIMHISNRFNGVPYLAYAAAQGLDVFAYLKAGKE
                     VQIENEVIVTEHLKRIKAYLAPGQNYHHVTYTPEEEESFKAGIASVVIPVFNRPEFIG
                     PAIESVQAQTVQNIEIVVVCNGGDDDPTVPEVRRYMAGGDKYDSHKPPVRLIIHDMNI
                     LGLCFNDALQTSRAKYYMQLDSDDLLFPEAVEKILKVYAEDDRIGMVIGSYQVYEKKA
                     DGRVEPVLNAEGKPFIVTHDEWTEENGRNNLLRIGGAGAPRSIKIKVLQELGWFGMND
                     SPYSRNYGEDYELVNKCAERYRIGRVWDPIYKVVRHAGGTDHMIDQVTIDVNENAKDH
                     MRLVALRRRQKINGIEPTA"
     gene            61472..62836
                     /gene="glmM"
                     /locus_tag="PKM23_00280"
     CDS             61472..62836
                     /gene="glmM"
                     /locus_tag="PKM23_00280"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNY89932.1"
                     /translation="MPELKNLMISVSGVRGIIGDGLTPEVALHFAQAYGSQFGPGRIV
                     VGRDSRVTGELVKYAVWAGLLSTGCDVVDIGMATTPTTAMATERAENAGGIIITASHN
                     PREWNALKLLARDGLFLSPEAGAEILERVRQQDFRFVAWDKVGRVIPHPAAAKEHLDA
                     VAGLELVDVDLIRKRRFRVVADPCNGAGGTILPQLFDRLGCEAFFLNLEPHGRFPRSP
                     EPLAANLGELCAAVTAHGADLGIAVDPDVDRLALVSNRGIPLGEEYTLGLATDFVLSH
                     TPGEVAVNASTSLVIDDIAARYGSRVHRTPVGEIHVATKARTTGAVIAGEGNGGVIYP
                     ALHLGRDAIVGIALVLQSLAEKEKSLAELQSDLPQYVMVKDKIALPFDADAKAMVARL
                     IQKHAGDKVDLTDGLKFLYDHAWVHIRASNTEPIIRVIAEARSEEEARRRVDALKQEF
                     ASLQ"
     gene            62859..65252
                     /locus_tag="PKM23_00285"
     CDS             62859..65252
                     /locus_tag="PKM23_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY89933.1"
                     /translation="MADFEKLGMFYLGKEYDTEKKQLLDSHTLYDSKDLVTHGVCIGM
                     TGSGKTGLCISLLEEAAIDNIPALIVDPKGDMTNLLLTFPGLSAAEFAPWINEDDARR
                     ENKTPEEYATGQAELWKKGLEQWGEDGARIQRLRDAANFTIFTPGSTAGVPISILSSF
                     ACPSEETMLDREAVGDLISSTVTSLLGLIGIAADPVKSREHILLSNIIDHSWQQGKEL
                     DLAGLITAIQNPPFERVGAFAVDAFYPAKERFELAISLNNLLAAPGFSTWLSGEPLDI
                     SRLLYTESGKPRHSIFYIAHLSDSERMFFVTLLFNQMVSWMRSQPGTTSLRALLYMDE
                     VAGYLPPVAAPPSKKPLMIMLKQARAFGVGVLLATQNPVDLDYKALSNTGTWFIGRLQ
                     TQQDIEKVVEGLRSGAGDLDPRITRALSALGKRVFLMKNIHESDPVIFQTRWALSYLR
                     GPLTLAQIKKLPAPASTAQARAAVNQKAAGFGTDAEGRPALPPQIPEYFAPLRGALRP
                     EATLLYRPGVLGLADVAFVPGESQRFGRIAWLEEGPMPVDWEQAVEAPFSEDDLERNP
                     EENGAFAKLAAPASRPESYPAWAKSFADAIYRTSKLELYTSTSLKLTSQPGESERDFR
                     IRISQLAREKRDDAIDQLRKRYATKSATLQDRKMRAEQKVAKEKNDYHSQTMSTAVSV
                     GATILGAFFGRKKLSATTLNKAGTVIRKGMRTTQERSDIQNAEESLEVINQRLADLEQ
                     ELNQEIARIEEQTDPMQQPLEVVAMRPKKGDINVRLTALIWLPYWQTNDGKREPSYL"
     gene            65308..65751
                     /locus_tag="PKM23_00290"
     CDS             65308..65751
                     /locus_tag="PKM23_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNY89934.1"
                     /translation="MQNIKDLSGMTYKIIDHTADVGLELEAATLSGLFTAAADGLMHI
                     ICPRCLIQPVQSHALQVCGENLEELLVNWLSELNYLCQVQHFLTARVQIREIQERFLQ
                     AEVTGERSDPLRHAVRSEIKAVTYHRIEVHQEAADKWRGRIYFDI"
     gene            65795..67234
                     /locus_tag="PKM23_00295"
     CDS             65795..67234
                     /locus_tag="PKM23_00295"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNY89935.1"
                     /translation="MSTPIDRITDYLFEIPQTGKMRVPARIYATANMLPDILKDRAAE
                     QAAQVAWLPGIVGYSYAMPDVHWGYGFPIGGVAAFDMDKGIISPGGVGYDINCGVRLL
                     RTGLQRSEIESRLRPAVEALFRAIPSGVGSTGKLKLNEKEEKRVLENGALWAVRNGYG
                     GEEDLDKIEENGRMAGADPDQISARARERGRSQLGTLGSGNHFIELGVVDEIYDPRAA
                     AAMGLRLDQITLFIHTGSRGLGHQVCDDAIKEMLHAAQKYGIELPDRQLCCAPISSPE
                     GQRYLAAMSAAVNFAFANRQMITHWAREALMQAWSISPRDLDLQVVYEVAHNIAKVER
                     HTVAGVERPLCVHRKGATRAFGPHHPEIPAAYREIGQPVLIPGDMGRCSYLLTGTTRA
                     MSDSFGSACHGAGRVMSRTQAVKSARGRSVVRELAERGILVRAESSETIAEEIPEAYK
                     NVTQVVDAVAGAGLACKVAKLKPLGVIKG"
     gene            67675..68184
                     /locus_tag="PKM23_00300"
     CDS             67675..68184
                     /locus_tag="PKM23_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89936.1"
                     /translation="MAFPSSTASPLQSLTDKIGGFCGYEKSATREPSDLNLRRFLIAG
                     IDARLKDLEEIPSAAIEEDQRLLLEVTKSTRRKLITISESLSAPTYAGQDFFTRDHIA
                     EKRVAGLYTLEKSMLDELADIQLELAAFQAGPIDKITIEDHFLHISDSIDNLNQALFE
                     RECLILGDQ"
     gene            complement(68284..69648)
                     /locus_tag="PKM23_00305"
     CDS             complement(68284..69648)
                     /locus_tag="PKM23_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY89937.1"
                     /translation="MAQRVLIVEDDDITREILVSMLVSRGFACDQASNGQQALEMLGA
                     GEYDVVVTDISMPLMTGIELMEKSAELNLRASFIIITAYASLETALEALRKGAYDYLL
                     KPLNFEDVAIKVKKLLEHKELVQENQALRQEINAQYDFSNIIGKSPAIRKLFATIRKV
                     SDSDSHVLITGNSGTGKELVAKAIHYNSPRRKGRFVAINCGAITETLMESELFGHKRG
                     SFTGAVVDKDGLFKVAHNGTLFLDEIGDMSLTGQAKLLRALENREILPVGATAVLPVN
                     VRTIAATNHNLWQEVQAGRFREDLYFRLNVIEIHIPSLRERPSDIPLLVDHFIQKYNR
                     QMNRHVRGADPGVLEMIVKREWKGEVRELENFIERLMIFTTGEVIAMEDLPGDMQPVE
                     ASARRPREADSLRSAMEQYEREFIREQLIRNHYHRGRTAAALKIGEATLYRKMNEYGL
                     SDLE"
     gene            complement(69653..70405)
                     /locus_tag="PKM23_00310"
     CDS             complement(69653..70405)
                     /locus_tag="PKM23_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY89938.1"
                     /translation="MSDEKETAAGELDRTPLRDYLLQAEKMHAMSMLAPGFIHDAGTP
                     LMAISSIAQFLSAKSTDPLVEQNLKQIGQAVDRLTQILRTIVDFSRPLCTLREKVYLN
                     SLIMEAVRIVKYDRRLKYREVGTELEAAIPQVSASPDQLLQVIIALCLNAADALEAVP
                     AGSLQLRSWHEGDQVHLSVTDSGTGIPLPDQPHLFTPWFTTRGSKGSGLGLFISRAII
                     TAHGGTISLASEPGRGTCVEIILPALSEEEGV"
     gene            70585..72504
                     /locus_tag="PKM23_00315"
     CDS             70585..72504
                     /locus_tag="PKM23_00315"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNY89939.1"
                     /translation="MIARNFAQEKLAESTIKELGTLARQCRGDILKMTTLAASGHPGG
                     SMSSIDFYLTVFAMANVDPCNPFAPGRDRIVTSHGHTSPGVFAALGRLGFFAIEDAVT
                     HFRQTNSPFEGHVEREVPGVEWGTGNLGQGLSALLGLGLGARLQGHNIQLYGFMGDGE
                     QQKGQIAEARRFAVKYGMTNATVFVDYNQLQISGPIAKVMPQQIRAGWEADGWKVLEI
                     DGHDYQAIYQAIRQAHSLPAPVMILAHTVMGKGVPSMENDHKWHGQALPIEECRKALA
                     LLGLTDDLDALVERRKTHNPAVPLLRREHLSAVADPGTPRTYGAAEKNDNRSGWGNAL
                     EDIVAANHGRLPIAVFDCDLAPSVKTTAIAKNYPDIFFQGGIQEHNTAALAGACSAEG
                     VLTYWADFGVFGVAETFNQHRLNDINGTNYKVVCTHVGLDVGEDGKTHQCIDYVGAFQ
                     NLFGFKVIVPADPNQTDRATRYISATPGNFLLAMGRSKMGMVLDETGEPFYGGSYSFV
                     YGKADLIRSGGMGAIIAMGGMVGRAVKAWELLKAEGIEVMVLSMACPSDPDMEALARA
                     AKTGLIVTYEDHHVRTGLGSAVANALADAGLATRLIKLGIARYGSSGIPDELYRSQGL
                     DPENLARTFTSALKR"
     gene            complement(72600..74231)
                     /gene="gpmI"
                     /locus_tag="PKM23_00320"
     CDS             complement(72600..74231)
                     /gene="gpmI"
                     /locus_tag="PKM23_00320"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNY89940.1"
                     /translation="MDLQLKKLAHFSGRPGPLLLIIMDGVGLGREDETNAVFMARTPV
                     LDELLRSRFFTRLKAHGTAVGLPTDEDMGNSEVGHNAIGAGRVFAQGAKLVDGSIASG
                     KIFTTGIWQELVGRGKSGKTLHFIGLLSDGNVHSNIRQLFALLENCVEEGVRRVRLHI
                     LLDGRDVGARTALTYIQQTEELLTRLNQERGCDFRIASGGGRMVTTMDRYNADWTVVK
                     RGWDAHVLGRARGFSSAAEAVESMYAEDAEATDQYLPAFVIVDAAGKPVGTIQDGDGV
                     VNFNFRGDRAIEISRAFDERDFNAFNRERSPDVLYAGMMEYDGDLHIPHKYLVSPPEI
                     DRVLSEYLCAAGVTSFALSETQKYGHVTYFWNGNRSGYIDEKLEKFVEIPSDRIQFDK
                     APKMKAVEIAEQAGALLRSGRFRFGRVNFANGDMVGHTGIMEAAIEAVETVDECVGVL
                     LQLVRELRGIAVVTADHGNADEMFTVKKGVKSVKTAHTLNPVPFVIVDPEYAGEYELA
                     GLANQGLSNIAATLFNLLGYEAPADYDQSLIRIKA"
     gene            74422..75174
                     /locus_tag="PKM23_00325"
     CDS             74422..75174
                     /locus_tag="PKM23_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89941.1"
                     /translation="MTYKDILPLVHKNDEKILLILLGGVGGIQSGPNLLTELQQAHRP
                     HLNALARKSACGLLLPVAPGVTPAADAALRRMLGWEEEQAPTWTSGLGLASCALSASP
                     EYLALFTQAGINAIASPDEPAGLIDRACAEILKHDMVILHYAAPEKEGLAGGYYEKIK
                     AIEDLDRLIPQLLESAPAVVAVCGDHSCPSALGAISWHPVPLLIHALTARYDMVQTYD
                     EIACTAGALGTLRSAELLPLLLAHAGKLGPAH"
     gene            75239..75892
                     /gene="deoC"
                     /locus_tag="PKM23_00330"
     CDS             75239..75892
                     /gene="deoC"
                     /locus_tag="PKM23_00330"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNY89942.1"
                     /translation="MAGLIDHTLLKPEAVAARFEELCDEAARYRFATVCVNPAWVRFC
                     AERLQGSGIGLCSVVGFPLGNITPEDKAAEARRAVGNGATEIDMVLHVGWLKSGFASL
                     VLEDIARVVEASAPHAVKVILETCLLTEEEKIAACELARSAGAHFVKTSTGFGKGGAT
                     VEDIALMRRCVGEALGVKASGGIRDLPTALAMIAAGANRIGTSSGVTIIEEIKQLNS"
     gene            complement(75904..76875)
                     /locus_tag="PKM23_00335"
     CDS             complement(75904..76875)
                     /locus_tag="PKM23_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNY89943.1"
                     /translation="MAKRIIGLDGGGTKTLGVMIDENGRLLARATGDSSNIQAIGAER
                     LGESLAGLIAELVAIAGCRQEEVDHLYAGLAGAGRPADREAISAQVTSRGLARAHTID
                     TDASAALAGAFGGGPGIIVISGTGSICFGKDEKGTLYRCGGWGFKLGDEGSGYYIGQQ
                     ALMAALKDLDGRGPETVLRGHYEKRYHLASIDLIISQIYSDKIDRTEIASNAPTVFAA
                     AQEGDAAARQIIESAGREIGRLVAGAAKRMGREGGEVAVALIGSIFNQRDVLMPFMRE
                     EALKVVRSAEFIEPRFEPAIGSCIMGLQKEGVAVSPQLLANLEKALV"
     gene            complement(76889..77434)
                     /locus_tag="PKM23_00340"
     CDS             complement(76889..77434)
                     /locus_tag="PKM23_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNY89944.1"
                     /translation="MRMTTRTLSAMALMTALTAFGSLLRIPFYPVPISLQSLFPLLAG
                     ALFSPRAAAGTQAAYLMLGLLGLPLFSQGGGMAYLFQPTFGYLLAMPLTAALVARGVQ
                     SLARPPAMRSLALVMALGALFLLVFGTLWLYFSFAWITGRPIPFLKAIGAGLLLFIPG
                     ECVKVAAALLVARKFQSFLRS"
     gene            77730..80423
                     /locus_tag="PKM23_00345"
     CDS             77730..80423
                     /locus_tag="PKM23_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNY89945.1"
                     /translation="MEIRLTHPAIRRSTAAAALRSRWLLLVLFFAAATAAWPQRLLTI
                     PLPREAVQAPFSLVPEERVAGPTVGLALSGGGLRGIAHVGVLQTLSEHNIPIDYISGT
                     SIGSIIGGLYASGYSPEELWKIIEKLDLGDLLNDSPSRANQFLAEKQKQSRAFLQLRM
                     ANWRFYLPEAITPGQKLTNILTDLYLNAPLHESDFSCLRMPLKIQTTDLLTGSKIVLQ
                     QGNLIEAMRASIAVPLLLLPVEYNNSLLVDGGLLDNLPVEETRAMGADIVIAVDCTAN
                     LRARAEMELPWELMDQVTTIMQQGHNLEQLEKADLILSFKSFPASTTQHEVVRQLYET
                     ARAQTAEIIPQLKTLLEKRRAEERSALNSPLFRVERVSFSGSSHLGFTDLIDARSGRP
                     YTPADIQANLATLYETGYFEQVEAHLDAAGGDTTLVYDCMPYPELKEIDLQGNSVLVD
                     SVLLNPFAPLVGHPLNRHQAREALQQIIRLYREEGYSLASLRAVHFDRERGIARITID
                     EGRIARVRFEGQEMTRNSVLNRDFSLRSGDLFQRRQAQQAVNNLYGTGLFNSVTLLPQ
                     AGPEGWELIVRLHEKKYTILRLGSRYDLERNGRAFLEVAHENTFGSGNDIILHGQYGD
                     RDHKLFLQYNADRLANSDLSFQATARQEYAKRFYYKNLTGVGEYERRGNGAHISLGMQ
                     IARLGMLSGFIRLEKMNIHRLSGSIIDAGELNLNTIGLNSVVDTRDQIPFPTRGKYHT
                     FTYEVSSGKFLGADISFFKVQNQLTTYWTFRNRNTFSPRLLWGTSDLTTPFSEHYRIG
                     GEDSFYGLREEEWSGRNMVLGSLEYRYCLPWKSYLTFYLSARFDFGAVWENSVEVKGN
                     DFISGRGAALSLKTPLGPFSAAYGRASNGHDRFYFSAGYNF"
     gene            complement(80411..81181)
                     /locus_tag="PKM23_00350"
     CDS             complement(80411..81181)
                     /locus_tag="PKM23_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNY89946.1"
                     /translation="MWYRRVRAVGAAIAALAAAAVLLAAGLPPDAEEAARKGVVTFVE
                     GSAKKQRGTEAEWHRVLVDSTVISGERVRTFTESRAELEIARLDRVRMAPRTTIDILK
                     LYEESVEHQRESAIQLQSGELWAHVGKKSGGQRFSISTPVAAAAITGTTLRLSVDADS
                     CAELRVYSGEVVLSRVTAGDSLRKGLSSAPQQVQGPTEVAPPREVSLEEWRLIVRSMQ
                     RVRVDARGEVVQSGSFSREDPEEQSDWVNWNRERDQKL"
     gene            complement(81228..83660)
                     /gene="gyrA"
                     /locus_tag="PKM23_00355"
     CDS             complement(81228..83660)
                     /gene="gyrA"
                     /locus_tag="PKM23_00355"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNY89947.1"
                     /translation="MTDNSEKLVPVFIEEEMKHSYIDYSMSVIVARALPDVRDGLKPV
                     HRRVLYGMLELGLRPGSAYKKSARIVGEVLGKYHPHGDTAVYDAMVRMVQDFSLRYPL
                     VDGQGNFGSIDGDAAAAMRYTEARLAPIAEEVIRDIDKDTVDFIPNFDDSLKEPLVMP
                     ALLPTLLVNGAAGIAVGMATNIPPHNLREVVDALVAVIEKPDLKPEDLRRYIKGPDFP
                     TGAIIVGDEEIAAYFKHGRGKLTVRARVHVEEVSGGRKRIVVTEIPYQVNKTSLIERV
                     AEMVRDKKIEGIYDIRDESDRDGMRIVFELRKEADAEGVLKDLFRYTQMQTTFGAIMI
                     ALVDGQPVVLNIKQILTEFLKFRHDVVLRRCKYELEKAEARAHILEGLKIALDNIDAV
                     IELIKKSRDVDTARKGLMKNFKLSEIQAQAILDMRLQRLTGLERKKIEDEYKELIKLI
                     QRLKSLIGSKSLRMQLIKQELLDLKERHGDNRRTQIITRSGDGEQSLQEMVSDEEFIL
                     ALTARGRIGRFSPQDYGSETVSALLGEKDFLRFAFRSVSSQLLVFVCRSGRAYLSRTS
                     FIPFASDEVKGGEISRLLHFKSEEEVIACFEMPKESSERFLFVATRQGLVKKVNFSEL
                     QRIKEGGQMVIGLREDDALAGAEMTDGATELLLATRQGKSIRFSEEDVRDMGAAAGGI
                     KGIELEADDRVVSLVALVRDKAAVLSITHAGFGKRSEASEYGTIKRGGKGIVNYKCSE
                     KSGWIAAVAEAGAMDQFIVVTKKGKLKRIKAKEIKLSGRATQGAAVVNVVKGDEIVSI
                     FPSPDPQGKKRS"
     gene            complement(83682..85601)
                     /gene="gyrB"
                     /locus_tag="PKM23_00360"
     CDS             complement(83682..85601)
                     /gene="gyrB"
                     /locus_tag="PKM23_00360"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNY89948.1"
                     /translation="MNNVKNQTAYDASQIQVLKGLEAVRMRPAMYIGDVSVRGLHHLV
                     NEVVDNSIDEALGGFCDEIEVTINEDESVSVTDNGRGIPVDIHPEQNRSALEVVMTVL
                     HAGGKFDKKAYKVSGGLHGVGVSVVNALSEWMVVEVARDGGLYRQRYQRGIPDAPVKK
                     VGSRKSTGTKTTFMPDGEIFKKRKFSFEILSERLRELAFLNPNLKISILDVKTGKSNK
                     FKYKGGLSAFIEYLDQGHNTLTKRPVFLSGERENIPVDIAFSYNDSYNENIYSYVNNI
                     HTIEGGTHLVGFKAALTRTLNNFATKNKLLKNGDSTLSGDDVREGLTAVISVKVNEPQ
                     FEGQTKTRLGNSEVKGIVEGLVNEQLGKFLEQNGSVGKRIIEKCQVAARSREAARKAR
                     ELTRRKSALENDSLPGKLADCSWSESGRTELFIVEGNSAGGSAKQGRDRRFQAILPLR
                     GKILNVEKARLDKMLSNEEIRTIVSTLGTGIGRDDFQVERIRYGKIIIMTDADVDGAH
                     IRTLLLTFFFRHMRPVIEHGHLYIAQPPLYRVARGNEELYVYDDEELRKVQEKYKDQT
                     KNYSIQRYKGLGEMNPAQLWKTTMDPQFRTLLQVTIEEAFEADHIFSMLMGDDVELRR
                     KFIEENAQYVRNLDV"
     gene            complement(85622..85912)
                     /locus_tag="PKM23_00365"
     CDS             complement(85622..85912)
                     /locus_tag="PKM23_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNY89949.1"
                     /translation="MARDDSQHISRGLAQLVHQLGLEQRLKQQRVIDQWPQLVGEKIG
                     RISRPERLQDGVLYVRVASMTWRTELLFQKQTILANISSAMGEGLVKDIRFI"
     gene            complement(85902..>86360)
                     /locus_tag="PKM23_00370"
     CDS             complement(85902..>86360)
                     /locus_tag="PKM23_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89950.1"
                     /translation="YEPNVEVTPGSDPRACFSDRLKKMKGLEIARGVSLVGPHRDEFR
                     LCVGARTLRQFGSRGEHKSALISIKAAELFLLKSYLHTTPLLLLDDLYAELDQERGEK
                     VIELFSDGGQCIISGTSADFSSLQHRLHRQKDEYLFFVRGGRIERGENGS"
BASE COUNT        17730 a        26966 c        24654 g        17010 t
ORIGIN      
        1 cctcgggctg aatgggggtg gtttgatcgg tgagtgcggc gacaacctgg acattctccg
       61 cgaccttgcc ggagacattg atgttcagac tggaatttac cttcaggccg cgattgttgc
      121 cgacggtgat gccgcgggtg atgctgccgt tcttgttgag ctgggcggcg taatcgacct
      181 caccggcgct gctctccccc ggcagggatt ggccaggggc accggccgcg ccaaaaaccc
      241 tctgtacgag cggatggcgc tgtttgacgg tcgcgagggg aaagggattg attttgtact
      301 gcaggcggat gttaaccatc tgcggtacct gctcattgaa gcggatctcg cccttgagat
      361 aattcatgct gtactgctca cgggtcaggg gctggttgtc gagaaagacc cgttcggagc
      421 gtgaaaggat gagagaatcg ggcagaaaat agggtcctct ccggccctcg cccaggatga
      481 ccagatcctt ttcaaggagg gagatgccct caatcttgcc ggtgatctgg gcgcgggcgg
      541 gcgtcatcgc caggaccaga ccagcgatca cccaccacct gattttctga ctcatcatcc
      601 cttcactcaa ggaatctgca gccactgcag ctcgaagacc tggatgcgat ggttgcccgc
      661 ctcggcgaca aagaggcggt cgccactgac cgcgacatcg atgggattgg cgagggctcc
      721 cagcttgtcg ccggcgctgc cgatcgccgc cagcagcgct ccactctgag aaaagaccag
      781 cacctggtcc cggccggtat ccgcgacgaa aagagcgcca ttgcgcgccg tctggaggcc
      841 ggccggctcc ttcagaaccc ccgcaccgat ctcatgcaga taattgccga aatagtcaaa
      901 aaccacgatg ccgctgcgca gccggtcgct gaccaggatc ctgtcctccc ggtcgatggt
      961 gattttctgc ggttccgcca gctcgccctc cccctcgcta aagtccccga aggagtagac
     1021 cgcctcccgc aggccgttca gccgcagaac gcgcaggttc tcagcgtccg tgacgaacag
     1081 atcgttgtga atggagaggg cgatgccggc cggaaagccc agattgagct tgtcatccgt
     1141 tcccgcggga taataatagg catcgatcca gtgcaggtcg cgatcgcagc gaatgatacg
     1201 gcgattttca tagtcggcca cgtagaggtc gagtccattc tccgcgcaga ggtccgccgg
     1261 gcgatggaac tggttgatgc cccagccgaa gccgccggtc atggccagca cctcacccgt
     1321 gaccgagagc ttcaggatgc ggttgttgcc ggcgtcggcg agatagagcg ccccttcagg
     1381 atcgatggag agggccgacg gagagcgaaa ctgcccgggc tccccccctt ctttgccgaa
     1441 ggcatagaga aagcgcacca ccaggctgtc accggcctgt gccggattgc cccacagcag
     1501 cagccagagc gctgctgcag tcaggtgcgg aaaatgagtt ctgcctgtcc cgttcatatc
     1561 actccaaaaa gaaaaacccg aacttgttcg ggcgccctga attgctgaac catcactgcg
     1621 gaggggaacc tcatctgccc cggtctctgg gatagatgac ataattggag ctgcgccgca
     1681 tttccagata ggcacgccag cggtcatagg cagccatgac cgccttgtcg cccctggcaa
     1741 tctgacgcac ctcacggtcc cccagacggc gtccgccctg atccagctcc gcctcgagga
     1801 tgcgatcaag cagcctcagc tgcgcccggg tcagatttct tttgatatcg cggtcgttgc
     1861 gcgcatctgt gtcataaatg ggactggcac cgacaagtgt gtcccatggg ccggagagca
     1921 atcccgatct ggcgaccagg aagagagcgg ccaggatcag gaagaaaaaa aaagtcgttt
     1981 ttacagcgct gcggtccgga ccgcgtgatt tggcctttgc tttcgccatc cccaactcgc
     2041 ttgacagtgt ggcttgaacg gtgatttcgt tcgctaaatt aaagttaaac aatgcaaaag
     2101 tcaaggaaaa attggggcgg ataaaaatta agcttggaac ttcactcaaa ttgatgtaat
     2161 ttttagtcaa gggtcgattc gggcccgatg gccacagtga atctctgttg gagggtatcg
     2221 agcccttgct gcatcgcctt accgtttcag gaaatgcaat aaccatgccg caatcaggaa
     2281 aaggcggagg ctggtggaag gcagcgcgaa gcgcactccg ccggacggcc ctgctcttgc
     2341 tgatggggca gctggcctgt gcatggaatc ctggatttcg tcaaggcgtt tatcccggcg
     2401 aggatgtaga gcgtctggcc gcgaacacac gcggtgaacg cttcggcgag atgacccccg
     2461 aaacccttga acgcttcaag gccgagattc gcacctactg gcaatcgccc tatctttggg
     2521 gcggaaatgc gccatcgggc atcgactgtt caggcctggt tggccgtatc taccaaaggg
     2581 ccgccgccat cgatctgccg cgcaccacgc tggagctcta tgagattggc atgagtgtgc
     2641 aggatcaacc cctgcagttt gccgatctgg tttttttcag cttcggcggc aaccggcaac
     2701 ccgatcacgt ggggctctac gtcgcccgcg gctacttcat ccatgcgtcg gtaaagaacg
     2761 gggtgaccct ctccctgatc accgaacagc cctatctgaa ccgcttcatg ggtgcacgcc
     2821 ggctgctcca gtgagcggtc cacatcaacc agagtcgagg aacagccaaa cccgaaatgg
     2881 acggccaaaa caaagaaccc atcctgctaa aatccaagcc cgcagctggc gtgcacaggg
     2941 gagatctggt cgaattacga ccaaccgatc tcgcgctggg gggcgccgcc gtggcgcgcc
     3001 tggacgatct ggtggttttt gtagaaaatg ccctgcccgg gcagaccgtc ctggccaaaa
     3061 tcttcaaaaa gaaaaagaac tttgccgaag cccggctgga gaaggtgctc acacccgcac
     3121 ccggccaggt cgaaccgcgt tgtgcgcatt tcggccactg cggcggctgc cgctggcagc
     3181 actttgccta cgggcagcag ctggagagca agcagcggca ggtggaggag accctgcgcc
     3241 atctcggagg cttcgccggc ttgaccgtgc aaccaaccct gccttcgccc gaaatctacg
     3301 gctatcgcaa caaaatggag ttttctttct ccccgctgcg ctggctcccc ttctttgaaa
     3361 aggggaggga ggatgcgaca gcggaagcgc tctatctcgg gctgcacgcg aagggatttt
     3421 tcgacaagat catcgacgtg gagagctgcc atctcctccc gccgttgagc agtgagattc
     3481 tgcaagaggt gcgtcaattc gccatcgaat ccgggcgtcc tgcctatcac gcccgcgacc
     3541 atcgcggctt ctggcgcttt ctggtggttc gtcacgccag gaaaaccggc gaactcatgg
     3601 tcaacctcgt cgcctccgag tatgaccgct cgctggctga gacctttaca cagcggatgc
     3661 aggccagatt tccgcagatc agcagcctgc tttacagcac cacccagagc ctgtcaggtg
     3721 tcgcgttcag cgaagccgaa tatttgctcg ccggcaaatc aaccatcacc gagaacctgg
     3781 gcagcctttc gtttgagatc tcgagcaatt cgttttttca aaccaatacc ctgcaggctg
     3841 aaaggctcta tgatgtcatt atcgattatg cgcagctacg gccggatgaa accgtctacg
     3901 acctctactg cggtgctggc accatctccc tctacgtcag ccatttggcg gagagagtgg
     3961 tcggctttga atcggtcaaa gccgcagtgg atgatgcgca ccacaatgcc gcgcataacc
     4021 ggataggcaa ctgctctttt gtttggggtg atctccggga cgccctcgag gataccggaa
     4081 aaaccatcgc acgctatggc cggccggatg tgctgatcat cgacccgccg cgtggtggaa
     4141 tgcatcccaa aacggtcgcg gccgtcctgc ggctcgcacc gcaacgcata gtccatgttt
     4201 cctgcaatcc ggccacgctc gcccgcgacc tcaagctgct ttgcgctgag tcgtaccggc
     4261 tgacgaaggt acaacccgtc gacatgtttc cgcataccgc ccacattgag gtggttgtgc
     4321 tcttgcagag aaacccttca tccgcacagg atgccacagt ttaaaacagg accgaatcca
     4381 tgaacatctt caagaaactc ttcggcaaga ccgaaaaaaa aaccaccccg gcaccgcgtc
     4441 cgcagtccgg ctccgaccgc catgatcagg gccgctccag gccgcgctac cagcaggggc
     4501 aaaggcccgg cagcagttcc ggttctaggc ctgtgggggg atctccccag cgccctggcg
     4561 gacagcctcc ccggcccaag cccgccccct cccaggagcc ccgcacctcc gagagcagcg
     4621 aatcccgccc gcagggcagt gcctcctcac gttcgcgccg ccgccgcagg cctcgtcgtc
     4681 cccaggatag accggcccag ctgcaatcgg cctcacagca gaccacgctt ccgctgcaaa
     4741 gccccggagt gaccccgcag cccaagcccg aggcggtcga aacccctgcc gctgtggttg
     4801 aagcgcccgt acgcgtgccc aaagagcccc tccccgagat cacccttgag cagctctccg
     4861 aacccatcgc ggcgggattg aaacgggcca actgggaaca gctcatgccg gtccaggcgc
     4921 ggaccatccc ctatctgctt ggccatcggg acctcatggt gcagtcccgc accggcagcg
     4981 gcaagaccgg cgccttcatc ctgcccatgc tcgaacttct cgatccctcc cgggcggaaa
     5041 cccaggccct ggtcctcgtg cccacccgtg agttggccaa acaggtgaac ggcgatgccc
     5101 agctgctctt cggggattcc ggcctgcgct cgaccgccgt ctatggcggc gtcggctatg
     5161 gcagccagat cgattccttc cgcgaggggg cccatctggt ggtcggcacc cccgggcgca
     5221 tcctcgatca tctgctcaaa ggcaccctca atcttaaccg cctcaagctg ctcatcctcg
     5281 atgaagccga ccgcatgctc tcgatgggct tttatccgga catgaaagag gtccagcagt
     5341 acctacccaa ggggatcaat gcctacatgt tttcggccac cttccccccc ttcgtcctcc
     5401 atctggctga cgaattcctc gacgatccgg aactcatcag tctgagcggg gatcagattt
     5461 atgtcgccga aaccgaacac tcttactata ttgtcccgcc gatggacaag gaccgcgccc
     5521 tggtgcgcat catcgagatg gagaatccgg cccaggcgat catcttctgc aacacccgct
     5581 cccaggtgca ttatgtcgcc atcgtcctgc agcgcttcgg ctataacgcc gatgagctct
     5641 cctcggatct gccccagaac gcacgtgaga aggtgatggc gcgtctgcac aatggcagcc
     5701 tgcgcttcct ggtcgctacc gacgtcgctg cccgcgggat cgacatccca cagctgccgg
     5761 tcgtagtcat gtacgagccg cccgaggatc ccgaggccta catccaccgg gccggccgca
     5821 ccggccgcgc cggcgccagc ggtgaagccg tctccctggt caccgaggtg gagcagatgg
     5881 atctggatcg catcgcgcgc aagttcaatt tcatcctcat cccgcgggtg ctcccacagg
     5941 acgaggatgt ctcggcgcgg gtcacagaac gcgtcacagc cttgctcgaa gccaaatacc
     6001 gcgccaagga caatgtcgag aaggaacggc tgcgtcgctt ccagcccctg gcgcagagcc
     6061 tggccgacaa cagcgacctc aacagcgtca tcgccatgct gctcgacgag ttctatcagc
     6121 agctcctgca cgcccccgtg cagaccacgg acagcgtcca ggctgaggaa cagcagatcg
     6181 agagggagag ggaaagggag agcagccagc cccaacacag gaacaaccgg ccccgccgcc
     6241 gcggtggacg ccaccgcagg cccggcggac gtccacaagc caatcccatc gagtaagctc
     6301 caatccgaaa ccactcggcg cgctggcgaa cctttgccag cgcgccttcg tctttcagcc
     6361 agcagaggtc attcacatga aatatctcgg agcccacgtc agcgccagcg gcggactgga
     6421 aaacgccccc ctcaatgccc acgacatcgg cgcgaaagcc ttcgccctct tcaccaaaaa
     6481 ccagcggcag tggctcgcca aggatctcac caccatagag attgaccgct tcaagcttcg
     6541 ctgctcggaa ctgggctatt ctccggacta catcatgccc cacgacagct acctgatcaa
     6601 tctcggccac cccgagaaag agggagtgga aaaatcccgc gccgccttcc tggacgagat
     6661 gaagcgctgt gaggaactcg gcttgaccct gctcaatttt catcccggct cccatctcgg
     6721 ccagattccc ctcgaaagca gcctccagcg cgtcgccgag tccatcaaca tcgccctcgg
     6781 caaaacccgc ggggtcagcg ccgtcatcga aaacaccgcg ggacagggca gtaacctcgg
     6841 ctcaagattc gaagagctgg ccgagatcat cgcacaggtc gatgaccaaa gccgggtcgg
     6901 cgtctgcctc gacacctgcc atctcttcac ctcgggatat gatctccgca ccgcggaggc
     6961 ctatgccgca accatggaac attttgatgc cgtcatcggt ttcgaccggc tcaggggggt
     7021 gcatctcaat gacgccaaaa aggaactggg tacacgggtg gaccgccatg ccccgatcgg
     7081 ggagggctat ctcggtcttg aggcctttcg tctgctgatg caggatcccc gttttgatga
     7141 catccccctg atccttgaaa cacccgaacc agagcggtgg ccggacgaaa tccgcctgct
     7201 ttactccctc gctgcggaag cctaacccgc cccattatgg caaaaaaaaa ccaccggaag
     7261 ctgtaatccg cgcttccggt ggctgcatcc aaaaccatct ttgatcagaa gaccttgata
     7321 tcccccaatc ccaccctggt gatgaccagc agacgggccg aggcctcgtc gtagcggggc
     7381 gtctggtaag ccatctcctg attgaatccc gccgcctttt ggtcgaagac cttgatatca
     7441 cccagtccca catgcgcctg taccttgacc gccatacccg gcataagatc gaggtggaca
     7501 tcgcccatac cactgcgcag gtagagacgg tgttcgcccg gttcaagcac ggtctgggag
     7561 gcatcgatga cgactgaccc gacaagggtc ttgaccactc cgccgttgaa tccctgcacg
     7621 gggagtttga cccgcaaatc gcccaccagg gaatcccggt agaggctgcc ctcgaccggg
     7681 ggtgcagccg cggctatatc cgagggggtt tcatatccgc ttcccccagg acggcgatga
     7741 cccatgacca gatagagccc cagggcgatc agaccaaccg gccagaagtg gaagatccag
     7801 cggatgatat caaacagcga tggaccgtcg aaaagcagga gaaagccgat cacgatgagc
     7861 accaggccca tgataaacga tccccgatta gattgggagg ccatttttat cctcttgaaa
     7921 attcatcaaa tccgtgttca ctggtgatta tgtccttcct ttaccggctt gggtagccac
     7981 gaccctcatt ttctgcaata tatttttcgg tttcgatcgc cgcagcgcag ccggaggcgg
     8041 cggcagtgat cgcctgccga tagatggcat cctggacatc gccgcatgcc agaacacccg
     8101 gcacattggt atgggtacgg tggtcggtct cgatatagcc ggtctcatcc agccggacct
     8161 ggccggtgaa gagttcggtg ttgggcttgt gaccgataaa aacaaagacc ccgttcaccg
     8221 gaaaattgga tactgctccg gtcttgacat tttttagccg caggtgagtc actgcagtgc
     8281 cgcccataac ctcctccacg acggtatccc agatgaaggc catcttttca tgtttcagcg
     8341 cccgttcctg gaggatggca tccgcacgca gccggtcgcg gcggtgaata atggtcacct
     8401 tttcagcaaa gcgggtcagg aagagcccct catcgagggc gctgttgccg ccgccgacca
     8461 ccaccacctg cttgccgctg aagaaaaagc cgtcgcaggt ggcgcagtag gagactcctt
     8521 tgccgatata tttgtcctca ccggggactc ccagacgctt gggcgttgaa ccggtggcga
     8581 tgatgaccac acgggcttcg aaagtctcgg tcgcggtttt gacgatcttg acctcgccgg
     8641 tgagatccac gctctgcacg acatcgtaga gcatctccgc cccgaagcgt accgcctgct
     8701 tctcaaagag ctggtacatc tcaaaaccgc tcaccccgtc cggaaagccg ggaaagttct
     8761 ccagatcatt ggtcagtgta acctgccccc cctgctccag tccgctgatc accaggggac
     8821 gcagattggc gcgcccggca tagatggctg ccgtcaatcc ggctggcccc gatccgatga
     8881 tgatgacgtc tcgcatgact cgtttatctc ccatcctgtt taatagtacg ttgatttgcg
     8941 ctaattagat tcacaatcag ccacaagtat tcaaactatt ttcccggatc aggcgggatt
     9001 ggggatgtcg atgaattcga cctcgacccc tgttttgcgc atgaggttct cacccagagc
     9061 gcggatgccc agacgttccg tcgcataatg gccggcggcg aaaaagtgga ttcccgcctc
     9121 ctgcgcgaga tgcatggtcg gttcgcgcac atccccggtg atgaaggcat cgagtccctc
     9181 ctcgatcgca agggccaggt caccggcggc gccgcccgag cagtagccga tgtgacggat
     9241 ctttgccggt ccgaaactga aggcaaaggg ctcgtgatcg aagacataga ggagaagctg
     9301 ttgaagttcg gcgaaatcca attcctcgat cgagcccttg aggccgatct cgccaagggg
     9361 cgcgacgtta tgcatcccca acgccttggc cgccagggca ttgttgccaa cctcctcgtg
     9421 tacgtcgagg cagaggtggt aggccagcag gctgacatcg tgatccagaa gggccttgag
     9481 gcgctgtttg aaaggcccct gcaccacccg gctctccttg tcccagagaa tgccgtgatg
     9541 aaccaggatc atgtcggcct ctctcttaaa ggcttcctcg aacagggcaa gactggcgga
     9601 gaccccggtc acaatgcgcc ggacctgggt acggccctcc acctgcagtc ccacggggcc
     9661 gtaatccttg atctccccga ctcggagata atcggccaga taagtaacca catcatcgcg
     9721 caggagcata gaccatcctc tcgattggtg taccgccgga ttgcacggac aggccgtttt
     9781 tcttgaatcc acccagccaa cactaattta aagaccagga aatgaaacgc aagcaaaatt
     9841 gtttccaaat tcactcccgt tttagaagcc ctttggagtg gcggctgcgc tccgccccgg
     9901 ccgaccgcgc cgctgcatac ctatccaaaa agcacttggc tttgtttcta ttatatccta
     9961 tatttaacaa aatatcgcgc cgccggcccc cggacggcgt acagatcttg atcatgccaa
    10021 ctttgagtcg cttgtggaaa tcagatataa atcggtgagc ggcacccgcg acgtccttcc
    10081 ggaagagcaa ccctactggc gcttcattga gaaaaaaatt gccgagatta ccgctctttt
    10141 cggctaccag cgggttgatc ccccaatttt tgaagaaacc cctcttttca ttcgcggggt
    10201 gggtgagacc accgacatcg tggagaaaga gatgtacagc tttctcgaca agggcaaaac
    10261 cccactgacc ctgcgccccg agttcactgc cggggtcatg cgcctctacc tgcaaaacgg
    10321 gctgcatatg ctctccaagc cggtcaagct ctattcgatc ggtccggtct tccgctttga
    10381 acgacctcag gccggcaggt tccgccagca cacccagttc aatgtcgaag ccctcggcga
    10441 gcaggatccg gccatcgatc tggaggtcat gtcggtcgcc tggcagctct acgccgaact
    10501 gggcttctct gatctcgcct ttcagctcaa cagcaccggc tgtccgcagt gccgccccct
    10561 ctacatcgag aggctcaagg cctactaccg ggaacatctg catgagatct gtgaggactg
    10621 caagcgccgg ctggagcgca acgccctccg tctgctcgac tgcaaggtgg ccggctgcca
    10681 gccggtcatc gctgccgccc cggtgatcag cgaacacctc tgccccgagt gcgacaatca
    10741 tttttccgcc ctgaccagct atcttcgcgg cctgggcaaa ccctacatca tcaatcacaa
    10801 actggtgcgc gggctcgact attacaccaa gaccgtcttt gaggtttggg ccaaagggat
    10861 cggcgcgcag aatgcgatgt gcggaggggg ccgctacgac ggactgatcg agcagctcgg
    10921 gggggatccc actcccggta tcggcttcgg atcgggcatc gagcgcatca tcctcagcat
    10981 gaaggagcag ggtctcactc ctgagccgct gcccgcgccg cggattctcg tcgctcatgc
    11041 gggcagcgcc gccaaaacgg ccgccattga agtagtcttc cggctgcgca gccacgggat
    11101 cggcgcgctc atggccttcg gcgagcgcag ccttaaatcc cagatgcgcg aggccaaccg
    11161 gctcaatgcc ggcgtggtca tcatcatcgc cgaacatgaa gtggaggcgc agaccctcac
    11221 ggtcaagaat ctgactgacg gctcccagga ggagattccc gccgccgatc tgatcttgca
    11281 cttcaagacc ttgggcctgc actgagccgg aacggccgac catgtccctt tccagacagc
    11341 agatcaggga tctgcgccgt ttgcagcaga aaaaatatcg cgaagaggaa cgggcctttc
    11401 tcatcgaggg ggtccggctt tgtgaggaag ccctggcggc gggcgctccg gtggaatgcg
    11461 cctttttttt cccggatggg atggagaatc cgcgcgcccg cagcctgctt caggccctct
    11521 ccggcaggga aattccgctc cagcccgtag acgccaccac cctcgccgcg ctgacagaaa
    11581 cccgcagccc gcaggggata gcagccctgg tccgtgccgg cgaaaccggg ccgttttcac
    11641 ccgccgggaa ccgggaaccc ctcgtactcg ccgtcgacga cctggctgat ccgggcaatc
    11701 tcggtaccct gctgcggacc gcggtgtggt tcggcgtcaa aagcctgctg ctcagccgtg
    11761 acaccgtaga gcttcacaat cccaaggtgg tccgcagcag catgggcgcg ctgtttcgtc
    11821 tgcgcatcca cgagggaatc gatctggagc agacggccgc agcggcccgg tcagccggat
    11881 ggcggctgct cgccgccgag gcggccggag gccagccggc gcaccggata tccggcggcg
    11941 ggcgcgatct gctcatcatc ggcagtgagg cgcacggcct caaaagcggt ctggatcaac
    12001 tgattgatct gcgtatcacc atccccggca gcggcggcgt ggaatcgctc aacgccgcca
    12061 ttgccgcggg catcctgctc taccaattaa ccggctccca ctaaggccaa tggattacga
    12121 tggaacctga agtcgacaac agcctcctcg atgcccgggc gctctccaga atcatgctgt
    12181 ggacgctcgc catgttcctc ttcagcatct ttttcggcgc cgtgatggtc tttctcgccg
    12241 gtcccaaaac gggactgctc gcagagaccc tcttcattat cccggcgctg ttatttgtct
    12301 acaggcggag aatgcctttc gcgcgcacct tccggttcaa tccggtctcc ccgcagatcc
    12361 tcttttatac cctgctgctc gcactgccag tcatgatact gggtgatgaa ctggaccgtc
    12421 tcatcgccat tttcttcccg ctgccctcct ggttcgatgt cggggacctg atgtcgattc
    12481 attccctcag cgatggcttc ctcatcatcg gcaacggggt tattgtggct gctattgccg
    12541 aggagatgct cttccgcggc ttgattcagc gttcgcttga gcgcatgcac gacatcgcca
    12601 cggccattgc cctttcggcc atcatcttcg ccatgttcca tttcaacatc tggtggatga
    12661 ttcagatcac cctgctgggc ctggtgctgg ggtatatcgc ctggcgcagc gacagcatct
    12721 ggcccgccat cctcatccac ggattcaaca acctgctctc gattctcgcc gccaatgccg
    12781 ctgaggagaa acttgcctgg tatacctccg gcgagcatgt ccactggata tggctcgccg
    12841 cctccctgct gctcctgatc ccggccttcg ccggctttca aaaggcctgc actgcacgcc
    12901 gcctaccggc tgccaccgac cgaaccatag gagatgacca tgattaagag catgcctcag
    12961 gtccgcatcg gcatgattca gtccgccgag accatccgct tccagtgtac ctccggcttt
    13021 gccctgctcg atctgcagga ccgccgcatc gcggagggaa cggctggcgc tgtgcaggaa
    13081 gcggtgatcg cccgggccga gagcgccagc ctccacttcc gcgtccgcct tgctatcgcc
    13141 ctgaccgggg aagaggcgca cctgctgcag gaaaagcttg ctgcccggaa tattgccact
    13201 ttcctctggc gccctggggt cgtgctcgaa ctcgccgatt tcgtcatgga caatcgtgag
    13261 tactggatcg tcaccgagcc tttcgccgac gaggccgccg ccaaatcctt tgccgcagga
    13321 tatgaacccg ttggtgaggc agtggtggtc aaagaaatcg tccgcaaggc caccggcacg
    13381 ctgagggtga tggatcaaac catcaccgat ggctgccggg tcgtcccgga ggatcccgag
    13441 gcccatatcc agctcctcga tgtcaccgtt ggcatcgaat ttcactggca gcataaacgc
    13501 actcagctcc tgcccggcat cctcgaaatc tgcttcaaca acgccggccg gcttcaagcc
    13561 atcaatgagc tggatgtcga gacctatctg atcagcgtca actcttcgga gatgaccaag
    13621 gagaatccca tcgagctgct caaggcccag accatcgccg cccgcagcac catcctggcc
    13681 accatgggca aacaccacta cgatgagaga ttccaccttt gctccgatga ccactgccag
    13741 tgttatcacg gcgtggccaa catttcggag cactcccgtt ttgccgcgca agaaaccgag
    13801 ggcgtcaacc tcatccacgg cggccgggtc tgtgatgcgc gctacgccaa gatctgcggc
    13861 ggcattatgg aggactatgg gcatatctgg gacaaccgcg aggtccccta tctcgtctcc
    13921 ggtgtcgacg gccgggaaaa gctggagtac cccctcgcca acgaaaccca ggtcaaagcc
    13981 tatatcgaca gctccccgga tgtctggtgc aacacccagc gctacgagat tccctcctcg
    14041 ctgccctaca ataccaggga gctcttccgc tggcgggtct cctatccgcg tctcgaactg
    14101 ggagagttga tcagccggcg gctgaacatc accttcggtg agctggtgga cctcattccg
    14161 ggcgaccgcg ctccaggcgg aaggctcatc cacctggatg tggtcggcag tgaacgcacg
    14221 gtgcgcatcg gcaaggaact ggtcatccgc cgcgccctct ccgaaagcca tctttacagc
    14281 gcctgcttct acatcgagcg cgaccgggac tccggggggc gggtgaccca tttccatttc
    14341 gtcggggccg gctggggcca tggggtcggt ctctgccagg tgggcgccac cgtcatggcc
    14401 caacagggct ttgactgtca ggctatattg agccactatt accgggacag ccggctggta
    14461 aaactctact aaaatccttt ccgcagaccc ggccctctgt cactatctta ctatagatta
    14521 gtacaatgca ggcccgccaa ctgactgcgg aatggtatga agatagtgat ttataacgac
    14581 gagctgcgct ccgggatgca gatgtatctg gccttgagca gccgtcatga tgtgcgcgtc
    14641 gcccaggatg tggatgatct tttcaccatg ctggatcaga ccgccgccga cctgaccatc
    14701 ctcgatctgg tctcgccggc gacccctgac gagcaggtgg atggcttcga catcgcccac
    14761 aagatccagg cccagcaccc ccaggtcaaa ctcgtcggga tctacgatca gggagatcaa
    14821 aacctggtta aaaaagcccg ggctaacggg attgccgagc ttatcgcccg accaattaaa
    14881 aaccgcgaac ttctcggact gatcgaaaaa tagggttggc gggaaaaatt aacagcacgg
    14941 gcatatgccg ctgtaaggcc ggtctcttta tcgccggcct ttttttatta acaaacctgt
    15001 tgcatctggt gtgcataagc tgtactttta ctgaaaaaga caagagcgat atgagcatgg
    15061 aaaccgattt tctcgtcatc ggcagcggaa tcggcgggct ttcggccgct ctcaagctgg
    15121 ctaaaaaggg cacggttgcg atcatcacca aaaagcacaa gtcggaatcg aacaccaact
    15181 acgcccaggg cggcattgcc tcagttttcg acaaggggga cacgtacgag ctccacatcc
    15241 aggataccct cactgccgga gccggccttt gccatgaaga tgccgtccgc ctgatcgtcg
    15301 agcagggacc gaagcgggtc aaggaactct tcaatatcgg cgtccctttc acgcgcaacg
    15361 cttctggcca gttcgatctc ggccgggagg gcggccatca ccacaaccgc atcgtccacg
    15421 tcaaggacca caccggccgg gatgtcgaga gcgctctgct cgaggccgtg cgccagaatc
    15481 cccagatcac cgtctttgaa caccatatgg ccgtcgagct catcaccgag catcatgtct
    15541 tcgccgcgcg gccggccacc gatctctcga tcaactgctg gggcgcctac gtgctggaca
    15601 tcaacgacaa ccggatcgag cagtttatcg ccagggctac cgtgctcgcc accggcggct
    15661 gcggtcaggt ctacctccat accaccaatc cctccatcgc caccggcgat ggcatggccc
    15721 tcgcctttcg cgccggagcc accattgcca acatggagtt catgcagttt catcccacct
    15781 ccctctatca tccggaggcg aactccttcc tgatctccga agcggtgcgc ggcttcggcg
    15841 ggcggctcat ccacaaggat ggggagtcct tcatggaaaa gtaccacccc atggctgccc
    15901 tcgccccgcg ggatatcgtc gcccgtgcca tcgacaccga actcaagaag cggggcgtcc
    15961 cctgcgtctt tctcgatgtc agccatctgg agcctgaggc cgtccgcaac cgcttcccca
    16021 acatctatga gaactgcatg cgttacaaga tcgatatcac caaagagccc atcccggtgg
    16081 ttcctgccgc ccattatgcc tgcggcgggg tcaggaccga tctcaagggg cgcaccagca
    16141 tcgccggcct cttcgcctgc ggggaggtga cctgcacggg ggttcatggc gccaaccgtc
    16201 tcgcctccaa ctccctgctt gaagccgtcg tctttgccca tgaggccagt caggccgcca
    16261 tccggtttgc cgccaaacgg aacaggatac ccactcccat cccccaatgg gatgacagcg
    16321 gcaccttcaa ccaggaggag tgggttctga tctcccatga ccgcctggag atccagaaca
    16381 tcatgtggga ttatgtcggt atcgtgcgct caaatgcccg cctcgagcgc gccctgagcc
    16441 gcatccgcct catccgccag gagatagaaa actattacaa gcgcacgcgg gtcaccgagg
    16501 gcctcctcga gctgcgcaac ctggccacag tggctcagct gatgatcctg tcggcgatgc
    16561 tgcgcaagga aagccgcgga cttcattata ccaccgatta cccgaaaagg gatgatgatg
    16621 cctttctgca tgacaccctc atagatcgct cgggtgtcta gccaaaccag gactcacttg
    16681 ccggagtgcg ccgatgatca tttcaaagat tgaaattcgc gccttcaagt cgatttacga
    16741 gctgaccctg ccgctggatc cccatatcaa tgtcctcatc ggcgccaatg agagcgggaa
    16801 atcgaacatc ctcaaggccc tcgaagcctt ccgggacaag gtggcattgg acgcctcctt
    16861 cacctgtcaa tactccaacc actactatct ggccaaggct ccagaactcg ccctggagtt
    16921 taccggcttc accaaggaaa accggcggct gctccagctg ctctccgaca ccttcaagga
    16981 ggtcgaccgg ttcaccatcc gcaaggacgg cccgaccctc aatgactacc atctctttat
    17041 cgatggcagc gcggtggagt cggtcgatat cgccaaactc ctgcgcgtac tgcccaagat
    17101 cctctacttt cctgacatcc ggctgctgaa gaaccgggtg gactttgacg atctccttgc
    17161 cggcctgccc gactgcgcca ccgaacgcaa cctcctgaaa atcggcggtg tcgacaacta
    17221 tgagttgatc ttcgaggaca atgcccgcgg acggcgcgcc cttgaggagg ccagccgcat
    17281 catcaccgag cagctgcgcc gcgtctggtc ccaggaaccc tcgatcgagg tcaaactcaa
    17341 cgtcaacggc cgctcgctct atatcgattt cagcgacagc accactgtct tcgatacccc
    17401 ggaatcgcgc agccttggct tccgctggta cctctccttt tatgtcaatt ttctcgccca
    17461 aaccttcgag gcccaggcca acgaatacct ctttctattc gatgaaccgg gcatccatct
    17521 ccaccccgcc gggcagaagg acctcatgaa ggtcctcgag gagctggccc ttaaaaatca
    17581 ggtggttttc accacccatt cgcccttcat gatcgatcgc cagttccctg aacgggtcat
    17641 cctggtcgtg aaagacaaaa acggcacccg cattgacaac caggcctatc gcgaccactg
    17701 gaagcctctg cgtgaagaga tcggcctgaa ggtcggcgat ctctttttct tcagcgactc
    17761 cagcatcatc gtcgaactcc ccaccagaaa gtctggcctc ctcgaaaaac tcaagggcaa
    17821 agccgagtga ccgcatgcca gctgtgacca tcgcacccgc atgcgggcgg caaagccccg
    17881 gcagcggtct gaaccggagt gagggcgggg cggcatgagc gagtcgaccg cctataagga
    17941 gaggtactgg ctctatcagt atcgcctcgg ccgcgaatac cttttgcccc tgctggcgga
    18001 gtgggggatc ggtctggagg ggcgccgcgt gctcgatatc ggctgtgcgg aggcgggcat
    18061 actctgcgcg gtggctgatg caggcgggag cagcgtcggc gtggaattga gtcccagccg
    18121 tctccggctg gccaggcagt tcgcttcccc cacccatctc accaggatgg agctcatcgc
    18181 cgccgatttt tttcacatgc cgctcaaacc ccaggcttgc gactttgacc tgatcctcct
    18241 tcgggatgtt ttcgagcacc tgcccgacaa ggcggcggcc ttttccgccc tcgtccggct
    18301 catggagccg gagacgcggc tcatcctgac ctttccgcct ttctactccc cctttggagg
    18361 gcaccagcag atgctcggcg gtcttctccg ccgcatgccc tggtttcaca tcctgcccgg
    18421 acccctgtgg tggtttttcg ccggccatat caaaaaacgc gatcccaacc cccgcttcct
    18481 tctcgagatg gagaaactgc gccggcacag gatgaccatt gccctctgca agcggctggc
    18541 ccggcggcat ggtcttgaaa tcgaggggca gagatactat ttcagccgcc ccagctacca
    18601 gctgcgtttc ggctggcccg tcatccaggc gcagacgata ggccgcatcc cggtgctgcg
    18661 cgaactcttc atcaccggcg ccgtcatcat gatgagaaga aaatggcccg cgtcctgatc
    18721 accacttact actttccccc tgccgggggc gccggcgtcc agcgcacact caaattcgtc
    18781 aagtatttgc gagaattcgg ctgggagcct gtagtgctga ccgcccggga cgccgactat
    18841 ccagccttcg atgaatccct ggctgatgaa ctccccgctg atctgaaaat ctaccgtgcg
    18901 cctctctttg agccttaccg cctctaccgc cgcttcaccg gcaaggccgc tggcaccgcc
    18961 acggacatcg ccaccctcac cagggatgac aaaagccagc agaaaacgag cgaacggatc
    19021 tccgaatgga tccgcgccgc cctttttgtg ccggatgccc gcatgacctg gctctacccc
    19081 gcctgtgccc tcggccgaaa aattattcgc gaggagaaga tcgacttgct cttctcctca
    19141 gcgcccccct acaccaccca cctcatcggc cgcaagctca agcggtcttc gggactcccc
    19201 tgggtggccg atttccggga cagctggatc ggctggctct cgacgccgca gtggcggccc
    19261 gctcccgccc gggccctgga gcgacgcatg gaacgcgagg ttctaacgga agcggaccgc
    19321 atcctcaccg tttcccctgg tgtcgaagag gacctgctca gccgccaccc cgcgctccgg
    19381 gatgcgcgct ggcgccgcct gcccaatggc ttcgacgcag ccgatctgga gggcatcact
    19441 ccggcgccca gatcgcccgg cttcaccctg atctacaccg gctccatgta cggcaaccgc
    19501 aatccccgcg ccctgctgcg cgccctcgaa cgtctgcagg atacgcgtta tccggggctt
    19561 gaagggctgc gcgtccgcct ggtgggccgc gtcggcgagc ccatcctgca ggaaatcacc
    19621 gcctcccctg cgggccgcca cttcgagcat atcccctacg tcacccacac ctcaagcttg
    19681 cagcacctgc tcgccgccga tgccgccctg ctcattatcg acgacgcccc cgccaaccgc
    19741 ggcatcgtca ccggcaaggt ttatgaatat atcggcgcac gcctgcccat cctcgccctc
    19801 gccccggaag gggatgccgc cgccctcatc cgcgatcatg gcctgggctg gtgcgctcct
    19861 ccggcggata tcgaggccat caccgccgcc ctccgcagcc tgcttgaacg acggcagccc
    19921 cctggggcgg agcggaaaga aacagcgtgg cagctcttcg aacgccggga acagacacgg
    19981 cagctggccg gaatcttcaa cgaagtggtc cgttccgcca aataaaaacg gccgctgcag
    20041 atgcaagcgg ccgctctcaa aggatttgcg cagggatcaa agggaaggct gttggtcggc
    20101 tttttttaac tcaaaaagat gggtcctgct ccgcccgatc gtgagacgca ggaaaggccg
    20161 gaccccgctc aggtagaaga ggggatggta ccaggcaatg cgggtttggt gccgcacaac
    20221 ggcaaaaccg gcggaatcga gcagatgctt gaagcgcagc tggtcatatt catggaaatg
    20281 ctggttgttc cagaaaagat ggggccgata gggcgtagtg agaaatatgc gcgtatcctg
    20341 gctgatatag cgatgcagac cgtcaagaaa gtggagcgga ttcatcagat gctcaatcac
    20401 ttcaaaacag aaaacatagt catatttctg ttccggcgcg ctccactccc ggtcaaaatc
    20461 tcccgaggta aacgagatcg gcagattgaa ttgttcggcc atgagagtgt cgaatcggtt
    20521 ttcggtcccg atggagagta cggctccgtt tccggttatg ccctcctgca cgaatttccg
    20581 ggtgaacttg aagcgcaccc tgccctgaaa atcacggaca tcgctcgaat cgacttttag
    20641 gttgagtttt ttggccatag atacctttcc ttgttgatga ccgggatgat tcgcaccgac
    20701 tgcgaacaag ttagaaaaat cctcaaaata atacaagcag atattctccc cggcgcatgc
    20761 gggcccggta tcggccatgc ggcccggggg agatcggaac ctcttgttaa tccacgagaa
    20821 actccttgat tctctaccac gattgcttta cattacccca tgcctaaaac ggtcctgttc
    20881 atcaaaccat ccaacagcac cttcattctc aaggacgaaa ccttcctcca ggaggagtac
    20941 cgcctcttca gccaccacta tgtgtatggc aacgccctcc gccacctctg cagcaccatc
    21001 catctgttct tctggctgct ccggcgtctt gccgcagcgg atgtggtgat cgtctggttt
    21061 gccgattatc acagctggct gccggttgtg ctggccagaa ttctgggcaa gccgtcgatc
    21121 gtaatggtgg cgggttatga tgtcgcccgc ctgccggagt tcaactatgg tgtcttcgtc
    21181 cgccgcttca ggggctggtg cgcgcgtatc gctctgcgca acgcggacca cctctgtccg
    21241 gttgccgcat cgctggtcga ggacatccgc acccacatcg gcgcggtgaa gggccgcatc
    21301 caggtcatcc ccttcggctt ttcagcgcag aaatggtatc cggctggaga aaaggagaac
    21361 aatctcatcc tcaccgtctc agtcggtgag gacctgcgca gaatgcgcat caagggactg
    21421 gatctctttg cagcagcggc ccaacggctg ccggaaggcc agttcatgat tatcggaccc
    21481 cagggagaga tgctgaacca tctgcgcgag ctgcagatcc ccaatctgca gctgctgccc
    21541 ccgcttcccc ccgctgagct gctctcccat tttcagcgcg ccgcagtcta tgcccaattc
    21601 tcgatccgcg agggcctccc caatgccatc ctcgaaggca tgctctgtga atgcatcccc
    21661 gtcggcgtgc ccgccggtgg catcccggaa gccatcggcg atgccggttt tatggccgcc
    21721 agccgcagtg tcgaaacggt ggtggaagcc ttgcaccagg ccctggcagc cgaccggcag
    21781 ctgggccgga aagcccgtga acggacgatg atcctctttt ctgaagagcg caggcgcagc
    21841 gccctcaggg agatcatcgc tggggcggcc cctcacgcca actgaaacct tgccgcgcaa
    21901 taatcgctgg attttatgga taaattgtct tataatagac cataagcagc atcttgatct
    21961 gtcaagcaat gaggctcccg tgaaaaagaa aaaagtcgca aaggaaaaaa ccctggcgcc
    22021 actgcaaccg gaaaaggaga ccatcatcag ccgccaccca ctggctgtgc ttctggtgat
    22081 cgccttcctc ctgctgctca tcttctatag ccaggtcatg ctggcgggga agacctttca
    22141 aatgcccgat cagcagagct cgcgtgcgac cgcgcctttt gtcaaggagg ccctcgcccg
    22201 cggcatcacc ccgctctggt gcccctacat attcagcggc atgccctctt tcggatcgct
    22261 gttgagtgct ccacacgtaa accgcctgga tgaattcttc accgggctct tccgcaccgc
    22321 catgatgccc gagttcacct tcatcttttt caattatctc ctcctggcgc tttttatgtt
    22381 tctgctcatg cgcgggctcg ccgtcgagcc gctgccggcc ctcttctcag gcctggcggt
    22441 catctttata ccccaattca tcgctttcac cgcctatggc cacaacacca aattcctcgc
    22501 cgtcgtgctg attccggtca tcctcctcct cgccaaaaaa atgatggagg agaaaaacgc
    22561 cctctggttc agcctgaccg ccctggccat cggcttccag atggtccgcg cccatatcca
    22621 ggtctcctat tacacctttc ttctcctcgg catctacttc ctctacaagg agatcgctga
    22681 tttccgccgc agcggggaat tcaaacccct cctcatctcc gccggacttt tggcgggagc
    22741 tgtcttcgcg ggggttctcc tttccatgac cctctatacc tcggtcctcg actatcagcg
    22801 ctactccatc cgcggcggca gcggtgaagg aggcgggctc gaattcgact atgccgccaa
    22861 ctggtccttc catcccctcg aaatggtgac cttcttcatc ccctcgttca tgggattcgg
    22921 cggcgccacc tactggggca aaatgccctt cacggattat cccctctatt tcagcgtcat
    22981 cgttttcctc cttgccggcc tggccatcct gctgcgtcgc gatcgtacga cctggatcat
    23041 gaccatcgtc gcgctatttt ctttgctggt ctccttcggc aagcatctgc ccctgcttta
    23101 cgggcccctc ttcaagcttc tgccttattt caacaaattc cgcgttccca gcatgatcca
    23161 tatcatcctg gatatcgcca tgctcatcct ggccggtatc ggcctgcagg ctctctttga
    23221 gctgcgcacc cagctgcctc agctcaaacc catggaacgc agccgcaaga tgaagggagt
    23281 gaaacgctat ctctatgcct tcaccgggat tgtcctcctc ctcgtcctca tcgtcctgct
    23341 cggcaaatct atctatatgg ggatggccgg aagcggggga cataccctta atgaagccca
    23401 gcgcctggag gcctacaaca aggctctgct cgacagcttt aaatccatcg gcctgcttgc
    23461 cctggctatc gtgctgattt tccagtttct caaaaacaac ctcacccgac tgactttgac
    23521 tctctccctc tcggctatgg tcatctttga tctgatcatg gtggatgcca agatcatcca
    23581 tccccgtgac aaatcggatg aaaaggcctt ttttgccgcc acaccggccg ttcagtatct
    23641 caagcaggag aaggagctat accggatctt tccggtcctg gatgacaaga gtggcaactg
    23701 gtacatgtac catctcatcc ccaacatctc cggctacagc gccgccaagg tccgcctcta
    23761 tcaggatttt ctcgacgaga ccggcttcgg ctcgcaggat cgctacggcc tcaacagctt
    23821 catgtccaaa tactggcggg tgggcatgcg cgataaccgc gtcactccgg tcgaggtgcc
    23881 tctcgatcag atcgccccgg agaggcgcgc ctttgattcg gccatgctcg acatgctcaa
    23941 cgtcaagtac ctggttctgc aatatctgcc cctcaacgac ccacgctacc agcccgtggc
    24001 cgatccagag cagatccctc tctaccgcaa caccaccgtc ctgccgcgcg catttttcgc
    24061 cgattccaca cggcagttca cgggccgcaa gaacatcttt gatttcatga agagcggccg
    24121 ttttgatccg cgccgggtgg cgataatcga ggagaatccc cccttcgccg tggcgccagc
    24181 cggcgccaac acggccgaga tcacccgcca tgatctccat cagatcgata tcgccgccca
    24241 ggtgaagtca ccgaccatcc tggtcctcag tgaaatctat tatcctgcag gctggaaagc
    24301 ctacgtcgac ggcaaggaga ccaggatttt caagaccaac tatctgctgc gcggcctctt
    24361 cttgcggccg ggcgatcacc agatcgtctt caaatttgat ccggcttcct accgggccgg
    24421 gaagtggatc agcacgctga ccctgctcct gttgctcgga ctgctcggat atgccctctg
    24481 gctgctttat cagaggcgcc gcaaacaacc gccggtgacc gcttgatctc gagggtgggc
    24541 ctttcgccca cccttttttt gacaaggaga gggatgatgt ccttccgcaa aacgctctac
    24601 atgttctccg gaaccctgcg cagcctcttc gtgctcgtgg tcatcctcag catcgccgcc
    24661 gtcaatttct acgtccaggg actggtccgg gatttgcgca agcagtcgcg ggacatcgcc
    24721 gaattctatg ccctcaccta ccagcggctg gcggtggcgg cggaccagcc cgaggtcctc
    24781 gactggctct tcgtcaacat tatcagccgg gccacctttc ccctcatcct caccgaccgc
    24841 gacggcaatc ccagttcctg gcagggtctt gccattcccg ataccgcccg gtcgcccgag
    24901 gcgatcaggc aggtgcgcaa ggtgctcgag aagatgcgcc aggagaatga gcccatcccc
    24961 atcacctacc aggagtacgt tctcagctat ctttatttcg gggactcgat gttgatcacc
    25021 cggctgcagt acctgccctg ggtcaccatc agcggtctcg ggctgctggt gctcgtggcc
    25081 tttctcagct tccgcagcat caagagcagc gagcagcgct tcatctgggt gggcatggcc
    25141 aaggaaaccg cccatcagct cggcaccccc atctcctccc tcatgggctg gcttgaactg
    25201 ctcaaatcct ccaaccacga ggggcccaac cgggaacaga ccctgacgga tatggaggcg
    25261 gatatcaaac ggctcgaaaa aatcgccgcc cgcttctccc acatcggctc cgaaaccctg
    25321 ctcgatcccc aggatctcca gccgatcctg cacgatgtct gcgcctacct gcgcaagcga
    25381 ctgcccaaat cgggaaatcc catagaactg atcgagaatt acagcgagct caaacccgtg
    25441 cccctcaacc gcgagctttt cgaatgggcg atcgagaacc tggtcaagaa tgccatcgac
    25501 gcggtgaagg gcaaacgagg aagaattgaa atcaccacca ccctgcttac gggcaaaaaa
    25561 cgcgttgcca tcgacatcag tgacaacggc gccgggatca ccgcaaagcg gcgcaatgat
    25621 gtcttcaagg cgggctacag cacgaaaaaa aggggatggg gcctggggct gaatttcgcc
    25681 aagcggatca ttgaagaata ccaccacggc cgtctcttca tcaaggagac ccatatcggc
    25741 aagggaacaa ccatgcggat tatcatatga gcggcacaga gagaagactg gagggaaggg
    25801 tcgcgctggt gaccggcagc gccaggggat tggggcgggg catggcctgc gccctcgccg
    25861 accgcggcgc agcgctggcg atccactatc gccaaagcaa ccgggaggcc gcccggctgg
    25921 tcgaggagct gcatgcgcag ggggttgaag cagacctctt taaggctgat ctccgccggt
    25981 cggaagaggc cggccggctg caggaagagg tccttgtgcg gttcggccgc ctcgatatcc
    26041 tcgtcaataa tgtcgggcca tttatcgaaa aaccatggaa tgaactgaag ccggcggaat
    26101 gggaggagat ggtggagggc aatctcaaca gcgtctatta tctgtgccgg gccttctggc
    26161 cggtaatggt ggcgcagcat tggggccgca tcattaatat cggcctggct aacagcgacc
    26221 ggattcaggc ctatcgtcag gtcctcccct acgccattgc caaaaccggc gttctcatcc
    26281 tgagcaaatc ccttgccgtc gaaggggccc cacaccatat caccgtcaac gtcctagccc
    26341 ccggcctcat ggacaacggc accctgaacg catctggccg ccgcgccgct gccgcgagga
    26401 tccctgccgg ccgccccggc accgccgagg atctggccgg cgctctgctc tacctgtgca
    26461 gcgaagaggc cgcctacgtc accggcgccc acatccccgt cagcggaggc tggggactct
    26521 agtgccgcac atcctccgga cgacgcggat agcgccgctc ctgatgcggc gctgcttccc
    26581 tactctcgcg cttgagagcc gccggcgttt tccccgcgct ctcgatcctc actccttctt
    26641 catccgcata gacatcatac ccgcctgacc agagcggatc caccctgccg ctgcgatggg
    26701 tagtgatgcg ggccgccttg aatatccggt tggccccctg cactgccttc cagattccct
    26761 ccgcggcggt tccggtcagt ttcacctcac tccccttgcg tgcactgccg ccctgaccgt
    26821 aatggccatc gatcgccgtc aggcggaaca tcgtgcgcag accgactttt ccgagcgccg
    26881 gtttccagtg cagtacctct cccttgagcg accaggcggc tcccttgatg ggataggatt
    26941 gcgccttgcc gggctggcca ttctcgtagg gaatcaggat gagactgaaa tcccccttgc
    27001 tgccgccctc cttttcgaat tccacacggg cgacgcgggt ctcacctttg agcggggtat
    27061 aatagtgaag atagaccgca aaaaagagca aaaccatggc cgaggcggcc agaagcagca
    27121 tcagcagcaa gctttttgag ggcgtcctgg actttttccg cgcaggcgcc ttcttcttgc
    27181 gccctgccga gccgccgaat cccagcatca taaaggctgc aatcgtggag accacaagca
    27241 gcaacagcaa gaccgtgttg gtatcggccg gcagtatctt gttcatatcc acctcattga
    27301 gcgatgatca catgttcatc ccaggccatg gcgccgaaaa ccccgatccc gccccggact
    27361 cgccagatcg ggacctcatc ctcctggaca cgcaggtatt cattataatt gcggtccacc
    27421 gccgccacct tgaggacata gcgatccggc tgtgccagat aaaaggggaa aaaatcgacc
    27481 gccgtcgaat cggtcttcgt ttcggcgttg tggccgctgg tgatgccccg cacgctgaca
    27541 atataaccgg gcgttccctg ctctccgcgc catgagatgg gcagagaggc ataggcctcc
    27601 acccgctcct gctcggccgg cgcgatgata tcaggtggaa ccggcatggt gcattcggct
    27661 tcaacccggc ggccatcagg caaaagcgcc aaaagttcgt aagttacacc ggggatgagc
    27721 cgcagctttt caccttcttc cgcatagatg ccgcccccct gatgcctgta tgccacccgc
    27781 tgctcccccc cctggataga gagctccgct cctgcaatgc cccggtcatc gggcagggga
    27841 tccatgctgc ggctggtctc ttccagacgg atgatcgtct gctgggaatt cagcagcaga
    27901 agggcgaaga gattgacctc cggttcgtaa ttgaactcat tgacatccgg cccatggtcg
    27961 cacgccagga gcaggatgaa ggccgggagg agtgctttca agagtctaga aggcaaagtc
    28021 cacccccacc gtcggcagca gcgggaacat ggtgaccgtc gtcaggcggg acggattgac
    28081 atcatgatcc cagaagtaga aaaagacatt cttgtgattg tagagattga tgatctggag
    28141 atagggcgag gtggagattc cgaagaggcg cccctgcttg cgcaggctta gatccatacg
    28201 gtgataaaga ggatagcggg cgctgtttct ccctcccggc cggtccgtta actcgccttt
    28261 gagagcgttt tcatcgagcc gccactcgta gtgcatgtac ttgccaggca tcggggtata
    28321 gggcatgcct gaaccatagg aaaagacggt gccgagggtc catccccggc cgagtgtgta
    28381 attgagcaca agatggaagg catgccgctg atcatacttg ggatagtaga atatcccatc
    28441 cgattgccgg cgggtccggc tccaggcgta gcccagccat ccctgcaggt tgcccgtctg
    28501 ccgcttgacc atcaattcaa cgccccgcgc cgtgccctca gcgacaaaat agtcatccgt
    28561 cgggtcagag gcaatcccct gttcattcag atcgagaagg ccggaaaagg tcttataata
    28621 gccttcgaag gtgaagatca gttcgtgcgg cagccaccat tccagaccgg cgacgtagtg
    28681 tctggcggtc tggggatcgt aggaagcgtt gatgggcagc cagagatcca taaaggcaaa
    28741 gttctgttca ccgctggcca cggttgtgag atattgggtg tagtggccaa agctcgcctt
    28801 gagggcgagg ttggaggtca gctgatactg cactcccagc ctgggcgaga ggaaccagcg
    28861 gtcgccgagg ctgtagtgtt cagcgcgggc tccggccttg agcatcagcc ggggatagag
    28921 ctgccagtgg tcctgggcgt agagggcgaa gagcgccgga cgggaggaat aggagaacag
    28981 ttgatagtcg ttgattgaga tgccgtaggt gaaatccaga tcggagtagg ctccgccgag
    29041 cttgatggtg tggccgctga ggccgaaata gttgaaatcg gtgcgggcac tgtaatcgtc
    29101 gatctcgttg ttaagggcca gattggccga gccgctgctg ctgacgaccg cactgagcct
    29161 gtttttaaaa cggctgtgcg aaagcagggt ctctgcaaag agatcgggac ggataatgta
    29221 gcgccagttc aggctggtgg tgcggttccc ccacacccag tccagatcca cgtccagctc
    29281 atcttcatcc tctgcctccg atgaaaaatc gaggacatcg tcaccataga aaccgctgaa
    29341 tgtcagccgg tggcgatgcg aaagatcgag attgatcttg ccctggaaat cgtaaaaata
    29401 atagggaaag gcgtagtcgc tctttttgaa gacctgatcg aagtaggtcc tccggccggc
    29461 gatcagaaag gagccggccg gaatggggcc ctcgaccatc gcccgggcgg agaggaggct
    29521 gatgctggcg accccgccgt acgatcgcgc atctccctcg cggttggtga tagagagcac
    29581 cgacgagagc cggcccccgt attcaaccgg aaagccaccg ctgagaaact ccagttcctt
    29641 gatcgcgtct acattgaagg tgctgaagac accgccgaga tggaagggat tatagacgct
    29701 gaccccatcc aggaggacga ggttttgatc cgggctgccc ccccggacgt agagttggct
    29761 ggaaaagtcg ctgcggctga ccacccctgg cagcagctgc agggtgcgaa aaacatccgc
    29821 ctcggccaga gtcggcactt gctgaagctg gcgcatggag agggcggtgg tggaaatctg
    29881 cacctcgcgc tcaaaacgct cccgctgtgc ggtccgggtc acctccccca tctggatcgc
    29941 cacgggggtc aaggcgatcg ccagcacagt gtcctggccc gcgcgcagcc ggagttcccg
    30001 ttgccagcgc tcatagccga gcaaagagac ggtgaagaga tagcgccctt cctccagatg
    30061 cgggatgacg aaatagccgc gcaggctggt gaggcttccg aaccgtgtcc catccagata
    30121 gacattggcg tagggcagag gttcgctgtt ggtggcatcg cgaacgaacc cgctgatgga
    30181 tgcgggtttg acctctgcgc ctgcttgcac cccgccaacc ccgagaagaa gctgaagaac
    30241 taagagaaga acggggggtt ttacgggtgg ggctttcatg gcggatggcc gcaggactag
    30301 ttttcgattt taactttatc gagttccatc ctgactgatc ccagcggcgc acggctcttg
    30361 atccgaaccg gcaagcagcg gtcgtccatg gagagccaga tccacatttc tcccttaagc
    30421 tcctccgtcg aaccggggcg cttgcccggc accagcagca gacaatcgaa tttgccggcc
    30481 ggcacctcga tcttttcctg tttttcgacc gtcatcgtca ggggatagac gtttttgtca
    30541 atatagctgt ccaggtcgat cttctgtccg actttcagat caagggtgcg cacataatag
    30601 atgatcgaga ggacatcctg actgtatgag ggcacggcaa gtgtatcctt accctcaaag
    30661 gcgcgagcat tgaggggatc gaagcgcgct ttgcggaagg ccttgaattt tccctcccgc
    30721 agaatccgct catatcccca cgggaagagc ccctcgcggt cgagaatgct cgtcatgacg
    30781 tcatccaccc gataaaagct ggagaaaaaa tcgttggtcc agagccgcat cgtgagcaga
    30841 taggtgctgc ggccgttata ttcctcccca tccttcagcc agagggatcc ccggccggcg
    30901 ctcatcttgc cgaaatgaac ccggtattcc agatattcgc ccgcctgaaa ctcctggtgc
    30961 ggaaaaaggc gcagttcctc cggattcgcg gcttgtgtat agctttgacc atggagagtg
    31021 aaaagggcgc cgcagagcat agcgagcgcc caggctgttt tattccagtt tttcatattt
    31081 tccccggcgg tatgaccgcg ctgttcagga gttgttcctt tacttttgca aacacccggt
    31141 cgctggttat taattccatg cagttggaat gggcacagcg ctctcccgtg cattttccgc
    31201 aggctggcag cggcggcgtc agcacagagt ccagatgtcc atacggaccc cagcgcaccg
    31261 gagcgcaagc agccagcggg cagtagagcc cgaccacggg acagcccacg gcgaccgcca
    31321 tgtgaagcgg cccggtgctg ttcgccacca gcagatgagc ccggctcaac aaggccaaaa
    31381 gctccttgat cgagagcgca ccatccagcc gcctgggctt gctctgcatc atgctgcata
    31441 accggtcaac gagcggggcc tccgctttgt tcccggtgat cactatcgta agagacagct
    31501 cctcctggat gcgatcggcc aggatggcga agccggagag gggccaatcc atcgccgacc
    31561 cgccgctgcc aggatggagg acaacaaagc cgctgccggg tgaaattcca agttcttcca
    31621 gccgcgacgc caccgcactg tgcgcctctt caggaatgtg caggtaaaaa ccgacgtgat
    31681 ctagcggggc gcccaaagca cgggccagat cgagattgag atccagctcg tgacggcccg
    31741 atttccggcg atggtgaaag agacgatgat tgaagaggaa agaataggcc cggtagccag
    31801 tgccagtccg gtgccgaatg tgggccagga gacagagcag ggccagcctg agggagggat
    31861 ggagaagaag gacggtatca aaacgacggc tgcgcaccag gcgcagcaga cgtaaaaatc
    31921 ctgccaggcc ggcatgctcg cccgagccat catcgagcag gatctcgtcg gcatgggggt
    31981 gcccgctgac cagctcccgg gtataagggc gcaccatcat ggcgataaaa gcggagggga
    32041 aggcctggcg cagcgcggtg aggaccggag tggagagcac gacatcaccg atccggtctg
    32101 tccggatcac cagaatccgc tccggtgtat agccgggggt tttcttgccc gtcgaggtca
    32161 atcgctttcc agcacagtat cgatctccag cctggagatg gcgccctcgc gcagtacacg
    32221 cacgggcagc ctggtcacat cgaccaccgt ggagggtgtc gtcgagaggg tttcggaacc
    32281 gtcgatgatc aaatcgagct catcaccgaa tttttcattc acctgctccg cagtcaccgc
    32341 cgggggctca ttgctccggt tggcgcttgt ggagatgatc ggaaaaccgc tgctcttgag
    32401 cagcgccagg cagatggggc agtcgggaat ccggacggcg atggtgtggg aacgatgaaa
    32461 gggcgagccc accagctccc tggatgcgcg aaaaaccatg gtcaagggac caggccaaaa
    32521 attctccatc agacgctctg ccgcctccgg tatctcctcc accagatcgc cgacctggat
    32581 gatatcggct gcaatgacga tcagggcctt gctgcgatcg cggcttttta gattgtagat
    32641 ccggtcgacc gcggcagcag aaaagatgtt cgaaccgatg ccgtagacgg tttcagtggg
    32701 gtagccgatg actccgtcct tgcgcagtat gcggacagcg cggctgatca gatcgtcctc
    32761 gggctgcttg gaattaattt tgatggtttc gctcatgctt gctcttccgc atgatgagat
    32821 tgctcagctc cgcaaagacc atttcgggcg gaatcccggt catgcattta tgcgtaccga
    32881 tgggacagct attgccgccg tgaatcgaac agggccggca gggcaattca atttgaacga
    32941 tcacagagcc cgcgctgagg ggaccaaaac cgaacttggg tatggtggga ccgaagagag
    33001 ccaccgccgc cgtgcccatg gcggaggcga gatgcagggg ggcgctgtca ttgctcacca
    33061 gcacctccgc gcgtcctatc agctcggcgg actgacgcag gctgagctcg ccggcgctgt
    33121 tgacccagaa tccggtcagc ccctcgctga tctccgtaca cagggtccgg tcgcccctgc
    33181 tgccgatcca gaccagacgc catccggacg ccaggaccat ctccgccagc ctgcgatagg
    33241 attccgctgg ccagcgtttg gttgcccaga tcgatcccgg cgcgatggca ataagcgcac
    33301 cggtcgtccc gcccaggctg gccagcatcc gatcgacagc acgatggtcg gcctcgtccg
    33361 ggaatatctc cggcggcacc agacccggct cttggcccaa cgctccgagc agggccaggt
    33421 ttctctcaac ctcatggcgc ttccggtcat aaaccacccg gtgagtgaaa agaaaagcag
    33481 cgctgctggt ggtaaagccg acacgcaggg ccgcccgcgt ccaccaggct aaaaacgcac
    33541 tgcgcaggga gcggtgcggt accaggacca gatcatacct ttcacgccgc agccgaccgg
    33601 ccaaggcaag cagggaacgc acgcccgcat cgcgcccgcg cttgtcatag agggtgagac
    33661 ggtgcagcgc gggattgttc tccaggctgt tcgcggctga cggaatgacc agggcgtcga
    33721 tcgacgcctg cggaaaaaga tcatggctcg cccggatcaa gggcgacacc agcacgacat
    33781 caccgataaa ggcggtttga atgatcagta tcttttcaat cttgtgcaac cggattccca
    33841 tcctctgccg gacccgccgg gggtgatgtc ttccatcgct tgtgcatcca cagccagtgg
    33901 tcgggatgga gacggatata gttttcgagc aatcgggcgt gccgctgggt aagctcgtga
    33961 atattctcgg gcgtgacccc ctgcaggtcc cgatgatcga tctcctcaag ctgaagtgta
    34021 tgccggcccg cttgcccgcg cacaaccacg ccgaagatga gaggcgcccc cgtcttgaga
    34081 gcgaaaaggg ccggcccgga cgccgtcgag gagggccgat tgaggaaatc gacaaaaact
    34141 ccctcgttgt gcgcatcctg atccgcgagg atggtcacga acttgttggc tttgagggcg
    34201 cgaaagatcg tgcgcacgcc catgcccaga tgggtgatct ccacccccca gccggttcgt
    34261 gtctgattga tgagcttgtt gaccagggga ttgcgctggt cgcgcgccaa tcccaccatg
    34321 ggaaatccca accggtgaat cacggcgggg aagaactccc agttgccaaa atgtccggag
    34381 agacaaatgg cgccgcggcc ttcatgcccc gcacgctcaa gcagttcacc acccacgacg
    34441 ctgatatact cccggtaaaa gcggtcgtcc atcttcggca tgcggatgaa ctcgacgatg
    34501 ttctggccga agttgcggta gacctgtctg cagatagcac gtacctcgtc atcacttttt
    34561 tccggaaagg aacgggcgat attctgcagc gccacctccc tgcggatggg gacgatatga
    34621 tagacaaagc cgcccagcct gacgcccaaa cgcagggcga gccgatgcgg catgagacga
    34681 accggaaaaa ccaggagctg gacgagcaga tactcgacga cattctggat ctgtttcatc
    34741 ttttaatgta ctgaaatctg cagaaatatt caagatctga ttccatcctc cccgccgggc
    34801 tccccttccg gcggctcctc ctcatcatcc tcttcatccc ggcgcggcat gagatggggc
    34861 gcaacccagg gggtctcgta tcccccctcc ccctcggcgg gcaggcgcag tacatccccc
    34921 tccccgagct ggcgggcgct ctcccattca cgcagctggc gccggttgcg ccggcggacc
    34981 agcaagacca caatgagaaa cagcaaggga agagcgagcc agatcagttc gtccacttcg
    35041 ctgagccaga gccacttttc cgttttttca agatggccgc gccattcctc ctcaaatccg
    35101 ctcatcgtgc ttccggtggc gcgcattaag atcgcatcac tgctttcgcc cgcggcgtac
    35161 ccatccagaa tggcgcgcaa agcctcgata tcataagtgg ccagcaggta gcgcaccgct
    35221 gaatagctct gttcgtaggc cagttgggcg cgatcgcggt gaaaattgag gacctcatcg
    35281 atctcctgca gcggtatcag cgatccggtg gcgagggcgc gcgagatccg acttctcttt
    35341 tcgtactgtt catgctcgga ataaaagatc gccaatccct cgtcgagcca gcgcggcagc
    35401 tcccggttcc cgagcctttg atgaagcagc aagtgtaaca gctcatgcac cagactctgg
    35461 ctgtaggagg attcagggcg gtcccagcgc ggggagcgga ccatcattgt cgaggtggca
    35521 ggcacagcga aagcctgggt ccattccggc atatggccgc gcagatagtc cctgaagctc
    35581 tccctggtcg gaacaatcat gacgcggagg gtatcgcggg gtactagttg cagatcatgt
    35641 gcaatctcct cagcggcccg ctccagaatg gaacgggtct cctccagcag cctgctgtct
    35701 tctatagtgt aatagataca gagagtaccg gagcagtgcc ggaggtagct cacgccctct
    35761 gttgaagccg tagccgcgaa ggaaaaggcg aggagaaggg taataatgag gagagccagc
    35821 gggactctca tagcgcatcc ggcctgtgca tatattcgct gacagcagcg cccatagcga
    35881 ggatctgttc catggcggct tcgcgcatgc actcgggatg atgttcagca cattcttgca
    35941 ggcggcattg cctgagttgg gccggacagc ccttcgccgc cctgagatcg ccccgggccc
    36001 gcagcgccat ctgcagctcc tcctggggga atctgcggcc gcgcattttg atgccgaatt
    36061 cgacggtctt ttcccacaca aagcggtggg cttcgagcag cccggccggg ggcagcgcct
    36121 ccagctgctc ctgcagaccg ccgccgcaga ggagcgcgcg cagcagatcc agctttgtac
    36181 tatagggcag cgccatgatc atcccgtctc cagcaaggtc attggcttca tgcctccata
    36241 ctcctcacag cttctccagc agcaggtcga gcagggtcgt caccaccttg cggacgcttg
    36301 ccggtttgtg cccctgataa aagagaaatc tggcggagga ggagagggca atgtgcggcc
    36361 gtactgcact ctcgggcacc ggcaggggtt gcaggccatg atcggccgcg atcacaaagg
    36421 gagccgcaaa agaggaggat gcgaaggtga ccatctcccg gcaggcggca taggcctctt
    36481 catcaaacca gttgaagcag agcagatacc ccagcatctt gcggccgacc agatcccagg
    36541 cgaagctgcg atcatagccg atgccgtaac agtagagcac cagttcacgg ttgatcggca
    36601 tttccccgag caggatgccg gcctcggtct catgaaggat gggacaaaaa gagtgcagaa
    36661 atgcgccttt gccgtcaccc tccggtccga cgacgacaat ctcagccata cgctgaatgc
    36721 cgctttcttc ggatcgtcgc tgcataggga tgagacctta ttgaaggggc cgggaacacc
    36781 ccctgccgcg gcggcaggga gatgatgccg gcagataatc agggtcgatg ggcgagaagc
    36841 ttctggcgaa taagcagcag atccgcccgg tgcagaaaaa gggtatgggt caggttcggg
    36901 gaaccaccac cgggcgcatc ggaaccatca gccttcaaga gaggtttttt ttttgatggc
    36961 cgttgcgggt gaaaaaagag gccatgcggg agtagggttc ggccgacctt tggcgcgatt
    37021 ccgaaacctg ccgtatcatg ctgctggcga tgagatccgc atccgaggcc tttcccggtg
    37081 aaatcacgat caatttccgc tcccaaagcg cctggatcac ctcgatggtg cgcagctcat
    37141 cctcgccgag tgaggcgacc agcaaccgga tgggctgcgg ccgttcgagg agttcccaga
    37201 tgcgcgcctc ccgctccggc agctccgggc ggaccacatc cgctgcgcgc gcgacataac
    37261 tctccacaga gggcagctgc gcactccgct gcctgtactc gtggaggcgt tgtgccccgg
    37321 ccagcagcag ctctcggttc gacctgataa tcctgcgctc acgctgcact ggctgcatcg
    37381 tcagttcgaa atagccatgc agccacaaca tcagattatc gagcgcctcc tcggcggtag
    37441 aatccctcag cactgcatcc acaacctccc cctggtcgac gaaaaggtag ccctcatcgg
    37501 gctcctgcac cagatgaatc atcgcggatt tcttgccgag ttcgagggtc tggatcagat
    37561 ccatgaggct catctcttcg aggctgcccg tgaaaccctg gttcaaggga acatccccac
    37621 cgggtgtgac cgcgctttcg cgcagaatcg attccacacg ggccaccacc tcttcgggat
    37681 aatagggttt ggcgataaaa tcatcgatct ccagggagag aagcttgagg cgcgtctcca
    37741 gctccggcag ccgggtgatg aagatgaagg gggtgtcctg ggtgtcggca tgagcgcgaa
    37801 tccgcctgaa gagatcggcg ccgtcgatgc ccggctgctc gagctcactg agaataagca
    37861 gtggaggagc tgcgaaggcg tgctcaaggg cggcggcgct ttcagcgatg actatgctct
    37921 cgagatgatg gtcatgcagg gcgttgacca gctgcgtggt cgccgctgag gctgcatcga
    37981 caatgaggat gttctgcctg ggagtattag ggcctgacat gtccgtttcc aaacctcagg
    38041 aggtagtctg tatggtcttg gtccgcgcat cgcgccgggc ctgaggccgg taaaaagtct
    38101 caaagaggcg gaagatgatc cgccgggaat tgacgaccga gaaatcactg accagctgca
    38161 ggccgtcccg ctcccgcaat cccagcctct gacggatggt ctgcacctcg agcggttttt
    38221 tcaggtattc attatagcta aagacaatga tcgccggcag gctggttttt tcgcgcagca
    38281 cctggcggag gtagcgctga tacccccaat ccacactgcg gccgtcgatg aggaagataa
    38341 agccgagcag ggagccggcg aaaaagtcga tgaccggggc gaactctcgt tccagcgaga
    38401 gggcgatgag attgagcaga tggccaccct tgaactcggc ggtgccgagg atcaaatccg
    38461 aatcggcggg ctgcagggcg ctcttctcct gcaccgcatc gatggcgagg ttggcgatca
    38521 tctggcggca cagttcgcgg ttgttgccga gaatcaggat gctgccgtgg gcactgcgga
    38581 atttctcctg tatcctgaca tgctgttcca gttcgcgcac ctcgtccgcc ccctcctctt
    38641 cttcgggaac gggttcagaa ggcggggctt cggccaattc ctcttccgca ccgatttcga
    38701 gcagggcatc ggcgggatgg tgcggaatga cccggtcgag atcggaaaag accgatcccc
    38761 cctccaggct cctgctgccg gcgaaaagat cctggccctc ctcctcggcg cggcgaagat
    38821 cataaaagag atccttgtcg aagctgctgt cggaattcca cgcggaggaa tccggagcaa
    38881 ggggctggag cacacccggc tcctccacaa agaggggcgt ctcctctccg gcaggaaggg
    38941 gctccgcttc gggggagctc ccgtcgatct gctcgtgcag cagctcctct gtggtttccg
    39001 atccgatcgc tttttccaca tgttccagac cggaagaagg aaggactccc gcctgacccg
    39061 ccatctccgg cgcctgctcc actgcacggg aagtttcacc cagctcgtcg gccggctcaa
    39121 aatcccgcaa agaatggaga aatccgagtt cgtagagctt gagaatccgg ttcagagtgg
    39181 tgatttcatc atacttgctc tcatcgatga tccggccgag tgaatgggcg ccatcgaaca
    39241 gccggacaaa atactcaaga tcggaggcca attctttttt cgcgatgatc tttttaaagt
    39301 tttgcgtcgt caccagaacc gtctccagcg cgggcaacct gtgcaacagc tcttcgcgtt
    39361 gttccattct cttggctcct tgcagtaaca accccatatt gctgacgccg atctcatctg
    39421 tcccatcaac aggagcaaaa accatagtga agcggccctt tttccacgag agcattttca
    39481 ggaccgcctc ctcgctgcgc tgcaggccgg ctgaggcgcg aatgaccgcc ccctccttga
    39541 agactatccg ccccgaaact ccgttctcat tgttcagggt gagcacgccg cttttacgct
    39601 cggcgccgaa gacctcgatc aactcgaccg cgccgagatc ggccagacgg ccgctgaatt
    39661 ggctgcggct ccgcttcaag agctgccggt agcgctcctg gcgcttgagc accatctgca
    39721 cgcgcatgac aatctcacgg acgtggatcg gcttgatgat gtaatccttg gcgcccagct
    39781 tgaagctttt aacccggttc cagacatcgc tcttctgggt gagaaagatc actgagcagc
    39841 tgcggttgag gggcgactgc tgggcctgtt ccagaagggc atagccgtct attccgggag
    39901 cagatacttc agacaaaatg gcgtcaaagg aacgggcggt gatcttctca agggcttcgc
    39961 tatcgttggc ggcggtatcg acatcaaact ggagatcgag gaaattattg cgcaaaatgc
    40021 ggacattttt gggatcggga tctatgagca gaatactgag cggattcatg ggatgcattt
    40081 ccgggttgcc aatacaatga gtgtgttttt aaccgataaa aatttatcta aaaatatgcc
    40141 ctatgtcaag gtttttctta aaaaagaaag ccgggaaaac tcccggctct gtgtccttca
    40201 tcgtcattgt cgcatctgct ccagccgcct cagaggagca tctcctgaac ccggttcacg
    40261 agtttgggcc tgcccgcagc cctttttttc atcgaaaagt aaagcaggcg gttgttgaga
    40321 tattcaaaat tggtgagagg gatcttggcc gccccggcga gattgaaatc ggttttctcg
    40381 aacaacccgg gagcctcgag ccggcagggt gcgcttcctc cttctgcctt gctccagaga
    40441 aagagagaga cctcgttgtg cagggggtcg ctgtagagca tcaccccgat ctgatcccgg
    40501 ctcagattaa aatcggtcag gctggttttg ctgccgtcgc cgttggcgga gaaggccagt
    40561 gtagccaggt aagccttgcc ccgctgccgg tctttctccg ggatcgggcg gcaaatcagc
    40621 aaaccatatt tgaccttgtt gcaggcgcgc tttttgcaga aatccagcaa tccgggccgc
    40681 accggcctct ccaactcctc ggaaaggatg gcataaaagg cgtacaggtt ttgcatcttt
    40741 ctggcgcagc gcgggcagct tttaacgtgg accttgattt gctctctggt ctccgccgcg
    40801 gtcagttcgt caagacagta taactcgagc tctctggttc cggggcagtc gactttacga
    40861 tggcgctcgc tcatggtctg tgtttatcca ggctatggcc tggctccctg tttgttacac
    40921 cctcctatta ctaatatgta acatacagac aaaagcgagc aactacggaa aaattaaagc
    40981 atttcacaca aatattttcg aaattctttt ttcgccagct tggccagata ttcaaaaact
    41041 gaacgttcct gctgccgata cgccttctgg gtcagttcgg gcatatagaa tttgaggttt
    41101 cgataatagg aggaggtgtc ccgtttctga atgagatcat agagcacatc gcggagtgcg
    41161 cgatgataga tcgccgcctt tttcgcaggc agcttgtggg tgcgcagata ctggaaatcc
    41221 agccgcgacc agatgctctc catcaccacg ctgatatact cctccatctc cttgctttcc
    41281 agttcatcgg agggcgtcga aaccgattcc ccggcagcca gccgttcgcg gcagacatcg
    41341 aacttgacgt aacggaccag tttgaccaca gcgtccaggg cgcagaaatt ctgattgtcg
    41401 gcgagggaat gaatggcttc cagcaaacgg cggatggaga cggaaacgga atcgccggag
    41461 cggtagttgt ccagaaaaag cttgtgcagc atctcctcgg ggatcggcgg cagggtgcgg
    41521 cgcagataga gggccagccg ggcgctcggc acgcctccct gttcattctt cggcaccggg
    41581 ccgtgaaata catattcctt gcccagttca cggaaaagat gcaggtcggc agccgtgcgg
    41641 atcgccacct tgatgttgcg tacgatcttc gccccctccg gatcccgctc ccggaaaatt
    41701 cgcgaaagct cctgcttggt cttgcgcacg atcagacgcc gtgtgaggat cagaacctca
    41761 gcatcattga taaaaggcat ctctgcaaat ttgccttcgt aatatttttt cagctgcgga
    41821 aaacggcccg actcgtcacg gccgaaaagc tcggcgatgc aatccatcgc caggtcagcc
    41881 aactcgagat ccgcctccca ggcatctccg ctgatacgct tgcccagcat ctcctggtat
    41941 ttgagatagc tgagggaaat cttctgcgcc agattaatgt actggatcaa tccggactga
    42001 ctatagctcc cgtcgcagat gctgtacaca agctgcgtta gattcgcatt tttcatagga
    42061 tcgcgtcgca cttccttgtt catcaatggc cacccccctc caccagcacc tccccgcccc
    42121 gatcatcgcc cccatggacc ccaccgctca cgagggtaca gaatcccgct ggtcatcccc
    42181 gccggctgct gactgccggc gaccccgcac cccgcacaca atccggggtt cacatagcct
    42241 gcgcaaatcg tgtaccaaga attgtgctgc ggagcacttt ggtaaattat tgttattatt
    42301 gttttgaaaa gaagggggag caggttttac gggaaggggg ctgtgacagc ctgatacaat
    42361 gtgccgggat gaaacataaa aaaaggcgca aagcaatttt gctgtgcgcc cggtcacaaa
    42421 atcaaaatat catctagctc tttaccatcg ggagtccgta ttttttcgcc ttgcggtaga
    42481 tggtcgcccg cccgatgccg agcttcttgg cggtctggga gatgttgccc tcgcattcca
    42541 gcagagcctg gcgcagggcc tcctcctcaa gcttttcgat ccagctggcg agggtgttgt
    42601 ccgattcgta gatgcgcttt tcgcccaggg aacggaccgc cgccggcaga tccttggcgc
    42661 cgatctccgg gccgctggca agcaccatcg cccgctccat ggcattctcc aactcgcgca
    42721 cattgcccgg ccagtggtag gccatcatca gatccagggc atccggggtg atcccttcaa
    42781 tgctcttgcc ctcctgcttg gcgtactttt gcaggaaatg accggccagc agagggatgt
    42841 cttcgcggcg ctctcgcaac ggcggcagct tgatcggaaa gaccgagatg cggtagtata
    42901 ggtcctcgcg gaactcgccc tttttcatct catcctcgag atccttgttg gtggcagaga
    42961 tgatccgcac atcgaccttg accagctcgt tgccgccgac ccgctcgaac tcccgctcct
    43021 gcagcacacg caaaagtttg gactgcgtcg ccggggtcat caggccgatc tcgtccagaa
    43081 agatcgtgcc gccattggcc tgctcgaatt tgccgatacg ccgtccgacc gcaccggtga
    43141 aagagccctt ctcatggccg aacaactcgc tttcaagaag cgactcaggc agggcagaac
    43201 aattgacggc gacgaaaggc ttgccggcgc gcatcgtcga gtggtaatga atcgcccggg
    43261 cgatcagctc cttgccggtg ccgctttcac cctggatgag aaccgtcaca ttgctgttga
    43321 aaaccttctc gacggcgcgg aaaacatcct gcatcacgcc gctctgaccg atgatgttgg
    43381 caaaggcgta tttgctcttg agttcggagc gcagctcatc gacctcgcgt cgcagcgagc
    43441 tgttcatcaa agcgttgttc accgtcacca gcagtcggtc acgggcgaag ggcttggtca
    43501 aaaaatcgta ggcccccgcc ttcatgctcg aaaccgcccg ctcgatggtg ccgtgcgcgg
    43561 tcatcatgat caccgggatg tagggattct cttctcggat tctgtgcagg gtctcgatgc
    43621 cgtcaatgcc cggcatctgt atatccagca ggaccagatc ggggggattt tccttgatca
    43681 aacgcaaaca agcttcgccg ctcagggccg tctctatttc ataccgcttc tctttgcgaa
    43741 gacttgcctc gatcatcttg cagatattct tgtcatcatc gacaatgaga atatttgcct
    43801 tgctcgttgt ttcatccacc atgccactct ccaatattgg ctgagaggta tcgatgcttt
    43861 atcatgcgct gcttaaggta tcataccaaa attttgtcta atttgcaaga tatttttgtc
    43921 ttactttgag acagttacag gatcccgtgg ctgcggcaga gcgacagcag cagcttctcg
    43981 gcgtgtggac catcttccgg ctcgacagca acagagagat ggaaccagtg cggggggaga
    44041 ttgaaggcgg ggagcagctt gacccaatcc ttgtcggtgg tgatgatcac atccgcccgg
    44101 tggtgctccc gcactctttc cagatcacga atatcgcgct gcccatagtc gtgatgatcc
    44161 ctgaagcaaa tgaaccggac gacctcaaag cccatttccc gggcggtatt caacaccccc
    44221 tgcggtttgg ccaggccaca aaaaagtacg acgcgcagat cgggcgtcgg atcaagccgc
    44281 tccccggcgc gattgcggca gtccgtgacc cccaacctcg ccctgataga gggcaaatcc
    44341 gccgggctgt gaatgagcag atcatcgtgg aggcgggcag actcgttgaa ccagagcagg
    44401 tcggcgcgtc tcagggcctc cagcggttcc cgcagcggcc ccgccggcag cagccagccg
    44461 ttgccgagat cgctctgctc cttgaaggtg acgatatcga gatcccggtg cagccggcga
    44521 tgctgaaatc cgtcgtcgag aagaaagaga tccggccgaa agtgttggag ggcatgcctc
    44581 ccggcgcggg cacggtcgct gtcgacgacc acaacagccc cgggcagctg ctgcgccagc
    44641 agcatcggct cgtcgcccgc ctcctccact ccggagcgaa tcccctcgcc atccgccact
    44701 acgaccatgc cccggctccg tcgccggtag ccgcggctga ggatgcacac cctttggccc
    44761 tgctgctgca gccagcgcac cagatagagc accgttgggg tcttgcctgt gcccccgacc
    44821 acgatattgc cgacgctgat gaccgggcgc tcaagccgga acacctcagc cgcgccgctg
    44881 tcgaagcggc gattgcgcct cctgatggcc aggccataaa gcccggagag gggccgcatc
    44941 agcagggcgg gccacctggt ttcaaaatag cccattcgcc tcctcctcca gtttggccag
    45001 ccggccctcc acttcccggc gaaagagctc cagctcttcg ctgtcgagtc tttccggaac
    45061 ccggatgggc tcgccgtaga tcaggcggac acgggagagg ggccaccaga gcgtcatccg
    45121 gtcccagctg ttcagggtga tgggccgaga agccgcaaaa gtcatcggca gaagggggac
    45181 gcctgcccgc atcgccagca cgagcgctcc ggtcttgaag tgatggcgcg ggcctgtcgg
    45241 gccatcgggc aggatgcagc agatcctgtt gaggcggagg gccttgagca tgtcacgaaa
    45301 ggcctgtctt ccgccgcgcg tgcttgagcc gcgcactgtg aaatagccca gccgctggac
    45361 ggtccgggcg atcatctcgc cgtcgcgatg ttcgctgacc atggccacca ctccgagatg
    45421 gcgatgcaga tagagggggg caaccatctt gccatgccag ggcacggcga tgaagccctt
    45481 gccctcggca accagctggt tccagtattt acggttgcgg acgctgatcc tagcactctt
    45541 gccaaagaac aggatgagca gccaggcaaa cctggtcgca agaaaaaaga gcactttttc
    45601 cttgtttttt ttatccataa gagtatactg attttttgtg tcagagcctg tgcgagctat
    45661 cacactaccc taatttgtct catcctgatg cagaccgatc tgttcaagca ccaggcgggc
    45721 ggttttttgc gaggctccgg cctcacccag cctgttccgc acaccagcaa gcttgacctg
    45781 catggccgag cgcgccggct catcaaaaag gagccgctcc acgaccggcg ccatctgatc
    45841 ggggacgaga tcatcctgca ggaattcggg gacgaccgct tcgccagcga caatattgac
    45901 aaggccgata ttcgggatct tgaccagctg ttttccgatc gcaaaggaga ggggagagac
    45961 ccggtagagc agggcgaagg ggagatcagc gcaagccgcc tcgagggtgg ccgttccgga
    46021 ggcgaccatt ccggctgtgg catagtgcat gagaggataa tagtccgccc tgaccgccct
    46081 cgcccagggg gccgcggcga caagagattc aaactgctgc ggttcgaccg aggccgcctg
    46141 gctgatggcg acctgcaact cgggatggtt tcggcgcagc tgctccgcgg tctgcagcat
    46201 cggtggcagg agatggcgca cctcctggtt gcgcgagccc gggatcagcg ccagcagggg
    46261 gtggccggga ttgaggtcgt acttctccat aaactcataa cgcgagagtc cgacctccat
    46321 cccctcgagc aggggatgac caacaaaggt ggtcttgatc tgatagcggg agaaaaaatc
    46381 cacttcaaag gcgaaaagaa ccgccatctg atcgacaaag ccggccatct tcgccgcacg
    46441 tccctgaccc caggcccaga cctggggggc gatatagtaa aaggtggaga tccccagttt
    46501 tttaaccgcc ttggcgaaac ggagattgaa gccggggtag tcgatgagga ccaccacctc
    46561 aggacggcgg ctcttgacca cctcgatcaa ccggttgaag acacggcgga agtaaagata
    46621 atggcgggcg acctcgatga agccgatata ggcgagatca ttaacatgat agtagagttc
    46681 catgcccgcc gcagccatcc ggtcgccgcc gaccccgaac agctccagtt ccggttcgag
    46741 cccgcgcagg gctgcgacca gtttgccgcc gtggaggtcc ccggaggcct ctccggcgat
    46801 gagcatcacc ttcaacggca tggcaacctc agcgtacaag ccagcgcagc aggatcagaa
    46861 tcgtcaccgc tgtgaagctt tgcagcgaat ttcgttcagt gcgcagggct tttttaaggg
    46921 tagtcggcac gcgctctccc ctgccccagg aggtgaggcg tggaaagacg gagggcacat
    46981 tctcttcata ttcaccatac tgcgcgccga acttgttgcg cagaaactct tcctccaggc
    47041 tgatgatcgc tcggtactgc acataaaaaa ggagcaaaaa tatcaccatc agccagggca
    47101 tccagggcca ggccatgatg agaatcccgg agctgagtaa aaaattgccc gggtacaggg
    47161 ggttgcgcaa atgcccgtag ggtccgtcgg taaccagaac atccgcaccg acctctccgg
    47221 tcgtgcgcgt ggcgctgccc gcatagcgga ccgaccagag tcgaatcgac tcgccgatca
    47281 gggcgatgag cagtcctgcg gcaaaagagg cgggggtcgt tcgggccagc accagcgctg
    47341 cgataatcag cggaattggt gtgtagctgc ggtacttgaa aaagaaatca cgcacagtca
    47401 tgggagtatt ccctgagttg gatttaaaaa cgctcagccc gccgctttag cgggtgttgc
    47461 gggcgatgat gccggtgatc tcctcggcca cctgcagcgc cctcaatccg tcatccccgc
    47521 tcacgatggg acgggtgcgg ctgcgcactg ccttgaggaa ggcctgccat tcggcctgca
    47581 tggcatcccc ctccggcgcc ttgggctgct catagatgat atgccgcttg cgcttgccct
    47641 tgtcgatctg ccccaacaca acagtggtcg gctccccctc ctcggcgccg acaatgcgaa
    47701 aaacctccga gaggcgctgc aggaaatcga tcgagatgta ggcatcgcgc tggaatatgc
    47761 gcatcttgcg cattttgcgc tgcgaaatcc ggctggctgt cacattggcg acgcagccgt
    47821 tggcaaactg cagccgcgca ttggcgatat cgacctcgtc ggagacgacc gccaccccgc
    47881 tggcatcgat gcggctgacc tcgctcttga ccagactgag gatgatatca atatcatgga
    47941 tcatcagatc gagtacgacc gcgacatcgg tgccgcgcgg atcgaagggg gccatgcggt
    48001 gggattctat gaacatggga tcggggagca ggtgctcaag tgcgcggatg gcgggattga
    48061 aacgctcgat atggcccacc tggagtaccg ccttgttctc cgccgccagc gccgccatct
    48121 cctccgcttc cccgattgtc gcggcgatgg gcttttcgac aaagactgcc ttgccggcct
    48181 tcagggctgg caaggtatac tggtgatgca gggtggtggg aacagcgatc cccacggcgt
    48241 cgacctgttc cagcatctcc gcataagtgg agaaagccat gcagcgaaaa cgctctgcac
    48301 tctcccgtgc acgcgcatcg tcgatatcgt aaaggccgac cagttcggct tccgccagct
    48361 ggctgagggt gttgcagtga aaggtgccca gtttgccgac accggcgatg ccgattctga
    48421 ctctctccat tgaaacctcc gaattgatcc gcccgggccg ggatcaggcg gtaaagtgcg
    48481 cctcatccgc agcctcttgc gggaaaatat cctggcagcc caggcagcgg cggacaagaa
    48541 aatagtcgct ctccttgcgc agcgaggtga tcatctcccc aagcagacca atggagaaaa
    48601 actgaatgcc gacaatgatc agcagcacgc caagaaatag cagcgggcgg ttgctgagaa
    48661 acctgttctc gaaaatccgc tgccaggtga gcagaatggt gatgacaaac cccccaagaa
    48721 agaagagaaa tccgagcacg ccaaaaaggt ggagcggccg catcttgtag cgggtcagga
    48781 aggagatggt catgagatcg aaagccccgc gggcaaaacg ggcgatgccg aacttggaac
    48841 tcccgtattt gcgggcgtgg tgcgtgaccg gaatctcggt gactcgaaag ccgttgcggt
    48901 gggcgatcac cggcaggtaa cgatggagtt cgccgtagac ctgcatggtc ttgacgacct
    48961 cccggcgata gatcttcagg ccgcagttaa agtcgtgcag ctggatgccg ctgaagcggg
    49021 aggtgaaata gttatagatc ttggaggtcc accgcttgat aaacggatcg tgccgcacct
    49081 ttttccatcc cgagaccaga tcgtaacccg tctcgagttt ggcgaggagc tgggggatct
    49141 ccccgggatc atcctggaga tccgaatcca tggtgaccac gaaggtcccc cgcgcgaccc
    49201 tgaatccggc ggccagggcg gccgatttgc catagttgtg catgaactgg atcaacttca
    49261 cccgctcatc cttgcgatgc agctcttcga tcaaccggcc cgatccatcg gtcgagccgt
    49321 catccacaaa gatgatctcg tagctctttt tcatcccggc catcactgcc gtgatcctgt
    49381 caaaaagttc ctgaagcgac tcctcctcgt tgtagctcgg aatgaccaga gaaaaatccg
    49441 gcggcacggt cttcatgatt gcattcatgg cgctcctcta ggcgatggaa ccggcgccgc
    49501 acaggtagaa ctcaccattt gatcctgtgc gcactggctg gaattttggt cttgatctcc
    49561 tgggcggaat attttttatt gatgctgatt tgatcatact gcaggatgag gtgctgtttc
    49621 tcggccgggc gctcgattcg tatggtatgc ggcacctgaa cgcccttgat gcggctgaaa
    49681 cgctcgaacc gctgccgcac aagcagtcgg ccggtggaat cgcaaagctc actccgggtc
    49741 accactccac ggccgggatc aatccagagg gtatgcaacc ccatggaggt tgtgcccgtg
    49801 agcaccagat gcttctcctc ctggccaagc accggctcag ccagcgcccc gcccctttct
    49861 aggccgacca gggtgcccag cagcaggtca taccctggcg cgatcctgaa aaagcgctcc
    49921 cagcgcagcg aatccatcgc cccctggccg tagagatttt cggtcgggag ataaaaacgg
    49981 tattcatgac ggtcggagaa gagccagccg acatcaaggc ccaaaagggc ttcgaggcgg
    50041 aggaagaggg tgtcgggttg gtcgataaga acatcgatat cgagcgagag ggaacggctc
    50101 ggagatgaaa gctgaagacg cccccgcccg ttgaaatgat cgattcgcgc ataattgctc
    50161 tcgagagcgg tgagtacccg ctgcggagaa gccgtatcgg cggggaggag ggctggccgg
    50221 cggaaagtcg cacacccgcc cgaagccatg atgaccgcaa gaaggagcgc gctggctccc
    50281 cgtgtaagga tggaatggaa cacccggttg tgaaccatgg cgatcaatca gtttttctct
    50341 tcttgcggga tcaatttcag tagtttggcc ttgacctgct ggcgctccgg ctcgagctcg
    50401 agggcccgtt cgtaggcccg ccgcgccggc tctttttcgc ccatggcgag aagaacgtcg
    50461 ccataatgct cccacacctc ggccgatttc ggagagagcg cgatcgattt ttcgatcctc
    50521 gcgcgcgcgc cgctcgcgtc cccctggcgg aagaggatcc agcccagggt atcccagtag
    50581 gcgccattct ccccctcgag cacgagagcc ctctcgacca gggcgcgcgc ctcgtccaga
    50641 cgcagattgc gttccgccag atggtaggcg tagttgttga gcacgatggc gctttccgac
    50701 tcgttcttca ccagggtggc gtagatcttt tccgcgcgct catgctcctt ctcttcatca
    50761 taaagcgccg cgagggcgag catggtctgc acattattgc catccacctc gagggatcgc
    50821 tccagatgat gccgcgcgca ggccttattg cccagacgat tgcagacaaa acccagataa
    50881 tagttgatct ccggatcatc gggaaagagt tcgaccgcgg ttttgagctg ctcctccgcg
    50941 gcggccagac tgtccatctg cgccagattg acccccgaga ggatccaaag cagggagaga
    51001 tccttgcgca gggagagggc cttgtcgaag cgcagccggg ccttgatgtt ttcatcccgg
    51061 accatgaaca ggcggccgag ggtgagcggc acgcgccaat cctctccgtg ggtcttttcg
    51121 agctcctcgc agagggcgat agccttcagg gtatccccct tttgcgccag cagattggca
    51181 atctcgatgc gcgcccccac aaaagtcgag tcgttgttgg tcagggactc ataaagggcg
    51241 accgcatcct cccagcggtt ttgggcggca tagagatcgc gaagccgccg gcgggaatcg
    51301 agaaagaggg gattaatttt aaccgcggtc tcatatactg cgacggcgga gagggtatcc
    51361 cgctgggccc ggcgcagcgc gccgagcgcc agatagccac gctcgctctg tgggtggtcc
    51421 cggatgatct tttcgaacca ctgcgccgcc gccagggtgt cccttttctg aaaaagatac
    51481 tgcccgagct gcaccttgtc gccgagatgc gttgaatcgc gggcaacaag ttcccggaag
    51541 agttcgaccg cctcttcgaa ccggttgctg cgctcgtaga gcagacgcag ctccgacttg
    51601 acctcctcga agcggttctg tctggtcagg gcctcgcgat agatccggat cgccccactg
    51661 gtatccttgg caaccttgct cagcgctgcc atggcgaggt aggaggcctc gaaatccggg
    51721 tcactcctct gcacgtcctg atagatcgcc cgggcctgat ccagggcacg atgctgaaaa
    51781 aaaagatgcc cgatacgaag gcgatattcg gggtccgcca gtccggcctc gaccagacgg
    51841 cggtaggaga gcagggcttt gtcgtaggat ttggagatga tctgcagggt ggtgaggctg
    51901 taaagactct ccatatcatc gggggccagg gtgatgatct gctcgtagac ctcggccgcg
    51961 tcgccgaatt cacgttgctt ttccagatta gcggcgagga gttccagagc gtcgatgctt
    52021 ttcgcatccc gctgcaacgc ctttttggcc atgcggatgc tggaattgta ctcttcgaga
    52081 agatagtaat tctcggcgag cgccaggtag atccctgctg aggaggaatc gatctcggcc
    52141 gcctgatgat actccaccaa agcattcttg ggttcgccga ggagatcctc gatcgtgccg
    52201 ttgatgatat gccgcatggc ctcgcggtta tagtggcgct gaactggagg agcgaccgcc
    52261 gctttgcggc tgcctgcaca gccgcccatg ccgatcaggg tgaggcaaag aagggtatga
    52321 aaaagtggtt tcattttgag cccagcctta aattaagaat aaaatttaaa gctatttagg
    52381 ctcaatcaca agagaaaaat cggccgctcc tcactactcc ggcagcttgg ctccggagga
    52441 gctgcggcgt tcgccggcca gggtaagggc gaccccggcc aggatgacca atccgcccca
    52501 aattgtcgcc ctgctgatgt tctcacggag gatgatgaag gcgagcaggg tggcgccgat
    52561 gggctcggcg agggtgaata tcgaaaccgt ggtggctgaa tagtgcttca gggcccagtt
    52621 gagactggtg tggccaatca cctggggaag cagggcgatg gcgaaaagca gaagccaggt
    52681 gcgcagatcg aatccggtca tcggctgccc ggaagagagg gtgacggtga gagcgaccgc
    52741 cgccgcgaca ctgtagacaa aaccgacata gcgcacggta tcgatatccg tgcgcagctt
    52801 gcggccgatc aggtaatagc cagccgcgcc cagggcgccg atgagggcga gcacattgcc
    52861 ggtcatcccg ctctctcccc cgccgccggc gcccaggctg atggccgcac ttccgcccaa
    52921 cgccaccacc atgccggtca tggccagtcg ggtcgggcgc tcccgcagga aaagccagct
    52981 gccgaggacg acgaaaacag gaaaggtttg gaccagcacc actgaaatgg cgaccgaagt
    53041 atagcgcaaa gaggagatcc aggcgagaaa atggatgccg agaaaaaggc cggaaaggag
    53101 ggcccagcgg cgcgaggtgg tcgtgaaggc atgccaaacc ggcttgcgcc ggaggcgagc
    53161 gatggccaga tagaccagtg cggcgatgag caggcggtag ctgctgatca caaagggcgg
    53221 cgccggacag agcttgatca gcaccgagga aagggagatg gagataaggc ctgcaccaag
    53281 aatgaccggc gcctgccatc gcacagcctg cctggccatc ccctattcct ctctcaccag
    53341 gggcagggac cacgaggtca tatcctcggg gaggtggctt tcgggaaaga gggcgcgtgc
    53401 ctgctcgagc agagcctggt cctccgcttc cccgatccgt ccgctgaggt gccacagaaa
    53461 aagccgcttc accccggccg cgcgcgccac catggccgcc tccgcagcgg tgctgtggcc
    53521 ggtctcctca gccagggatt gaagctcagc gccgaaggtg ctgtcatgca agagcacatc
    53581 ggccccccgg gcaagctcaa cgccggaggc gcagggccgg gtatcggtgc agtaggcgat
    53641 gcaggaaccc gctcgtgacc gtcccaccac ctggttggga gagatgcggc gtccgtcggc
    53701 aagtgcgatg ctctccccat ccaggagacg gcgccgcagc ggcccttcgg ggatgtcaag
    53761 cctttcggcc tcgccgctgt cgaagcggcc ggggcgtgga tgggcgcaga tgcggaatcc
    53821 gtaacagggc ttgctgtgtt ccagccgccg ggctgaaaag gaaaaacgct ccagctgcac
    53881 ctcgccggtc tcatcctctt cccattcatg aatatggact tcaaagcggg tgcgatggcc
    53941 ggtggcagag gcgatgccct cgagatagag acgcaggccc actggtcccc agatgtgcat
    54001 cggctccctg cgatcgagga gatgctgggt ggtcagcagg cctggcagcc caaagatgtg
    54061 gtccccgtgc agatgggaga tgaggatgtg gcgaatgcgg gcagcagaga gtccggccga
    54121 catcagccgc tgctgggatc cctcgctgca atcgcagagg atagtctcgg ccggaaaagc
    54181 gatggccagg gtgggcaggg cccggcgcag ggagggcaca gcagccccgg tgcccaaaaa
    54241 gaagagccgc aaggcgtccg ccatgggagt gcgctccacc tacttctttt tcggctgtcc
    54301 gaagcggttc tcggtgccgt tgagcagtcg cctgacgttg gtgcgatggg tatagacgat
    54361 cagcgcagcg gcgaaaaaac tgaagaggta aaagggcagc ggcacttcac gaccgagtac
    54421 gtaccggaaa atggtcagaa tgagcggaaa ggcgattgcc gcgctgatgg aggagaggga
    54481 gacaatgcgg gtcgccagca ggatggcccc gaagatcaaa aggcagtaga gcacagcctc
    54541 gggaaagaga gccagcatca tcccggcagc cgtccccact cccttgccgc cgcgaaaacc
    54601 ggcaaagacc gtccagatat ggccgaaaac cgcggcgatg ccggcgccga gctgcagcag
    54661 gtggggatcg gccagctggg gcgcgatcca ctgcggcagc cagtaggcag cgacaaaacc
    54721 cttgaaaaca tccaccaggg tgacaaacag ccccggtttc cagcccagca cccgaaaggt
    54781 attggtgccc ccggcattgc ctgagccatg ctcacggatg tcgatgccgc gcagcacctt
    54841 gccgacgatg atgctggtcg ggatggagcc gagcagatag gccagaacca gaacgatcag
    54901 ggcggtgaac atagatgtgc actctccaaa tgataaccat tattcgggta aaggatcacc
    54961 catgatagcg atggcgatcg ccccgacacc gacgtgcaag cccagggcgg gggaggcttc
    55021 cagtatggga atatcggcct ccgcgaattc ctgctgcagt atccggcgca ggctcaccgc
    55081 cagttccggc gccagcatat gggcaacgat gaaacgggga ttgatgagcc ggcgcgcgtg
    55141 cgcgagggcc aactccaccg atctttcagc cagccgcttg cggccgatca ccttgccggc
    55201 ctcgacgatc cgcccctcgg catcgatcga gatcagcggc ttgatttgca gcatggtgcc
    55261 gaaaaagccc tgcgtcttgc gcaagcgtcc actgcggacg agatacttga gggtcggaat
    55321 cgccacaaag atgcggcttc tgctgatcac cccggcgagt ttttccttga tctccgccag
    55381 cggcagcccc gcctcgatca tcctggccgc ttcgtgcacg atcaagccga gcgcagccga
    55441 ggtggtgcgg ctgttgacca cttcaatctt cagtttaccg gccagctgcc gcgcgccgtt
    55501 ataggctccc tggatggtgc cgctcagggc ctcagagatg tgaatcgata aaatggactc
    55561 gtagccctcg gccagctttt cataaagact tgtataaaag ccgaccggtg gctgggaggt
    55621 agtcaagcgc tctccgcctc tttcgaggat cttgtaaaag tcgtgggcgc tgatctcctc
    55681 ccgatcgcga aagctgcgct cgccgacatt gatcatgacc ggcaccacat ggatatcata
    55741 cttttcaatc cactcggcag gcatgtcgca ggtggaatct gtgaccagag cgatagggcg
    55801 tttgggggcc agtctggccg cctcatcata ctgcttttgc atgtcctcgg ctttggtctg
    55861 cagcagggtg ccatgtgagg cggcgagtgt aaagacggca tcaggctcgt tggtatggat
    55921 gtggatgcgc accttgtgcc cgccaccgac aacgatcagg gaatccccga gcggcagcag
    55981 ctccgcctgc aagcggtggc gatcgagttt ctccccctga acaaggcatt ctgtgcaata
    56041 gcggaagcgg atctcgtcat ccatcttgtg aaaatgaaaa tggcgcagct tttcgaccat
    56101 gtggctgccg gctcgataag cagcgatttt gccggaatca atgaaatcgg aaagcccttc
    56161 gagcagatgg acaaaaccct gggcccctgc atcgactacc ccggccttgc gcagagcctg
    56221 gagcttgttc ggggtgtcgg cgagggattc atgcgcccgc gccagcccat cccgaaaaag
    56281 gcggacaaaa tcctcctcgc ccagggcctg ctccttgaga aaagcggacc aatcctgcat
    56341 gacggtgagg atagtcccct cgcggggatt ggaaagagct tcgcgggctc gggctgcggc
    56401 cacggcgacc gcctgggcaa attcctccgg cgagagtctg gcgcgtcctc gtgccccctc
    56461 cgcaagaccc tggaaaaact gggccaggat ggcgccggaa ttgccgcgcg ccccggacaa
    56521 ggccccctcg gaaatgcagt ggcacacctc cgccagcgag gtgaagggac attcccgggc
    56581 actatcaacg atgctctgca tggtgagcgc catgttggtg ccggtgtcgg cgtccggtac
    56641 aggaaagacg ttcatattgt tgagggcgtc ctgcatctcg ccgatgcgag ccgaagaggc
    56701 aatgattgcc cgcttcagtc gcgcaccatc gatatatctg atcttgattt tcatgacccc
    56761 gctcgtcaaa taggtgcccg tttttccgca cttcagctgg taaaatgagt ggagaggaca
    56821 ccggtcgtta ttgcaccgct cgatgtgccc gccaggagca gtggtgatcg gccgaattgg
    56881 ctcctgagcc gcgaaaccag attgtgcaca tggtccgtag agcccgcctt ggccagtatc
    56941 accactgaac cggctgcccc ccagggcata accctcgccc cgtagatgcc ggcgggggtg
    57001 cccattcccg ccgcagcagc cgccagccag ccggcgtcgt cactgaccat cccggagatc
    57061 cggctgaatt gctcatgcga ggcggcgagc agggcacccg cctcgctgca gcgcgcctca
    57121 tccagaaccg gaccgccttc ctgcagcagg cgcacgaagg cttgcacgcg ttccgcctcc
    57181 tcgatggcga actcgagaag cgtgcggggc atataacgct tctcgggatc gatctcagcc
    57241 tctccctccc gggcgccggg gtggttgagg agaaactccg cgcctgaaaa gcgataaggt
    57301 atgagctttt tcagctccct ctgccagagc tcccgcgaaa ccggggtgga atcccccgcc
    57361 tctggtcgca gcgaattctt gcccgacagc gcctcgctta aaagggtgcg cccgatcagc
    57421 aggcttacgc acgtctcgtt cagtttcagc cactcagcgc tctggcgcac cccggtgtcg
    57481 acggccagga gctgcagatt ctgtggaaac gcaagaaatg tgctgatctc cggcggctca
    57541 ccctgcagca gggcgagctg cccctccctg ccgagcaggt agctctgaaa atcggacggg
    57601 ccgcatttcc ggcgcatgat ctcctgctcg atgcggaaac cccatcccgc caggtccacc
    57661 cgcccgggcg gcagggcata agcctcctgc agggccagca gcagggcggt aagctgcgcc
    57721 ccggtatagc ccagcccggc gccggccggc agagcgctct cgatggcgat attcacgcca
    57781 aagtcacggc tctttccctc caggacggga tagagcaggg ccagcgccgc cagcacgttg
    57841 ccggtccagc tcttctcctg ggcggccgag aggctgcgga gcatcctcca gtccggctgt
    57901 ccggacgctt cgagcagatc atccagacgg tattcaaccg tcaggacccg ttccctgctg
    57961 agattgagat tgcggatgag aatgcggcgg tccagcctgg gctggaccgc gacgatgacc
    58021 gcctgtgcga ggggataatg caagacgggc atgcccgcca gggcggtcgc accccccaac
    58081 agatcaagcc gtccgggcgc gcgcgcgatg aaaatgggcc gcccgggggc gaagaggccc
    58141 cccagatttt tctgcagaaa ccggcgaaaa ttcagcaggt ccgcaggcgg ttgagcttca
    58201 gccatggcag gatgcggctc ctctcgttta acccctgttc gacgctgacc agaagatgag
    58261 aaaccccgtt ggcaagaaaa atggctgtca atgccgcaag ccggcgattt gaaagctata
    58321 tctcggtccc atcgtgaatt acagcattct tcatcacaat ggtgatgccc ttgctgatcc
    58381 agatgccctc ggcttcccgc tctgcatcac taacgccggc gcgattgaga atgcgcacgt
    58441 tagcgccgat cctggcattc ttgtcgatga tcgcgcgcct gagctggcag ttggccccga
    58501 tgccgatcgg cgggatcccg cgggcgctct cctcccggcg ctcctcctcc gtctggtaga
    58561 aatccgcccc catgaccagg caatcctcaa tgctgcagcc ggaggcgatc cggctgcgga
    58621 tgccgatgat ggagtggcga atactggatt ccgcgatatg gctgccttcg cagatcagcg
    58681 actggacgat ggtgctctgc gccaccgagc tcggcggcag aaaacgcggc ctggtataga
    58741 tcggaaactc ggcgttgaaa aaatcaaaac tggggcgagg ctgcgtcagc agctgcatat
    58801 tggcccggta aaatgattca atggtgccga tatcctccca gtaaccgttg aagggatagg
    58861 cactgatctt cagttcgttg accgacgagg ggatgatctc cttgctgaaa tcggcgaaat
    58921 tgctcttgtc gcgcagcagg ctgatcaaga cctccttgcg gaaaagatag acccccatcg
    58981 aagccagata gggcctttcc gccgggatct cccgccaggg gccagaggcc cggcctgaca
    59041 tggccatgcc cttcaatccc tcctctccct tgggcttttc acggaattcg accaccttgc
    59101 cgtcgtcatc gacgcgcagg atcccaaaat cgccggcctg cgccatggag attggaacta
    59161 ccgagatggt gatgtcggcg ccgctccggt gatgccattc cagaaacggt cggtagtcca
    59221 tgctgtaaag atggtcgccg gagaggatga ggtattcgtc gaccttgtgt tcgcggaagg
    59281 tggagacata ctggcgcacc gcatctgcgg ttccctgaaa ccatagagaa tgctcggggg
    59341 tctgctgggc ggcgaggatt tcgacaaagc cctcgctcaa cggcgaaaag tggtaggtct
    59401 gggtgatatg acggttgagc gaggcggaat taaactgtgt cagcacaaag atgcaactga
    59461 tcccggagtt gatgcagttg ctgacgggga tgtcgatgag gcggtactgg ccggcgaggg
    59521 gcacggctgg cttggcgcgc atcttggtca gagggtagag acgggtccct cgtccaccgc
    59581 caaggatgat ggccaggact ctttgcatgg gtggccctcc ggccgttttc atggcgagtt
    59641 acagaagaaa ttttctgtaa cttacacatt tgccatctca atgtcaatat ctacttgaaa
    59701 cggcgcggcc gcggtgacgg aatccggcgc ccgccgcccc cagtcttccc gcatcgcggg
    59761 tgcacagccg cggcgagcaa aaaaaggttg aatgaatggc aacgactcgt tatattagac
    59821 ttggaaatgt caaaccgccc aacggccatc gcctccatta aaggagtacc ctatgaccac
    59881 caaactttcc attctctttt acgcttatcc gcagctgccc tatgaaaccg agtcctttga
    59941 aaaaatgctc gcctccctgc agaaccagta tgatcacaat tttgaggtgc tccttttcgg
    60001 cgacaacgac caggtgcgca aacgcgcgga aaaggccggc tatatcttca tcccaaccga
    60061 cctcgatcac aaggccaagg ccctgaacag cgccctgcgt caggccaggg gggagtatgt
    60121 gctgatctgc aacaacgcgg ccaatgccct ggaattcaag cgctccgcca ccaggttcta
    60181 cgcgatggcg atggaccgcc atccggagac cagcatgctt tatacagact atgacctgat
    60241 cgacaagggg gtgcgcaagg aacgcaaact gctggaatgg cacaacggtc gtctcaccga
    60301 ggcctgggat accggttatt gtctgctctt cagccgcgcc tttctggaga gggttggctt
    60361 ttgcgacgaa cactacagat tcaatcccct caacgatctc cgcttcaagg cgatggagac
    60421 cggccggatc atgcacatct ccaaccgctt caacggcgtc ccctatctgg cctatgccgc
    60481 agctcagggg ctggatgtct tcgcctatct caaagcgggc aaggaggtgc agatcgagaa
    60541 tgaggtgatc gtcaccgagc atcttaaacg catcaaggcc tatctggccc cgggccagaa
    60601 ctatcaccat gtcacctaca cccccgagga ggaggagtcc ttcaaggccg gtatcgcctc
    60661 agtggtgatc ccggtcttca accgtcccga gttcatcggc ccggcgatcg aatcggtgca
    60721 ggcccagacc gtacagaata tcgagattgt cgtcgtctgc aatggcggcg atgacgatcc
    60781 gaccgtcccg gaggttcggc gctacatggc cggcggtgac aagtatgact ctcacaagcc
    60841 cccggtgcgc ctgatcatcc acgatatgaa catcctcggg ctctgcttca acgatgccct
    60901 gcaaacctcc cgggccaagt attacatgca gctcgattca gacgatctgc tctttccgga
    60961 ggcggtcgaa aagatcctca aggtctatgc ggaggatgac cgcatcggca tggtgatcgg
    61021 ctcctatcag gtctatgaaa agaaggcgga tggccgggtg gagccggtcc tcaatgcaga
    61081 gggcaagccc ttcatcgtca ctcatgacga gtggaccgag gaaaatggac gcaacaatct
    61141 gctgcgcatc ggcggcgccg gcgccccgcg ttcgatcaaa atcaaggttc tgcaagagct
    61201 gggctggttc gggatgaacg actcccctta ttcccggaat tacggggagg attacgagct
    61261 ggtgaacaag tgcgccgagc gctaccgcat cggccgtgtc tgggatccga tttacaaggt
    61321 ggtccgccac gccggaggga ccgaccatat gatcgaccag gtcaccatcg acgtcaatga
    61381 aaacgccaag gatcacatgc gcctggtggc cctgcggcgc cggcaaaaaa ttaacggcat
    61441 cgaaccgacg gcttgatcga gggggaggcc catgcccgag cttaaaaatt tgatgatatc
    61501 ggtctccggg gtccgcggca tcatcggcga cggcctgacg ccggaggtcg ctctgcactt
    61561 cgcccaggct tatggcagcc aattcggtcc cggccgcatc gttgtcggtc gcgattcgcg
    61621 ggtgaccggg gagctggtga aatatgcggt ctgggcgggg ctgctctcca ctggctgtga
    61681 tgtggtcgac atcggcatgg caaccacgcc gaccaccgcc atggccacag agagggctga
    61741 aaacgccggc ggcatcatca tcaccgccag ccacaatccc cgcgaatgga acgccctcaa
    61801 actgctggcc cgtgacggcc tcttcctgag tccggaagcg ggggcagaga tcctcgagcg
    61861 ggtcaggcag caggatttcc gctttgtagc gtgggacaag gtgggcaggg tcatccccca
    61921 tcctgccgca gccaaggagc atctcgatgc ggtcgccggg ctggagctgg tcgatgtcga
    61981 tttgatccgt aaacgccgtt tcagggttgt cgcagatccc tgcaatggcg cgggcggaac
    62041 gatactgccg cagctgttcg accgcctcgg gtgtgaggcc ttcttcctca acctcgaacc
    62101 ccacgggcgc tttccgcgca gccctgaacc cctcgccgcc aacctcggtg aactctgcgc
    62161 cgcagtgacc gctcacggcg ccgacctcgg catcgccgtc gatccggatg tggatcgcct
    62221 cgccctggtc tccaacaggg ggattcccct cggcgaggag tacaccctcg ggctggctac
    62281 cgactttgtc ctttcccaca ccccgggcga ggtggctgtc aacgcctcca cctccctggt
    62341 gatcgacgat atcgccgccc gttacggcag ccgggtccac cgcacccctg tcggcgagat
    62401 ccatgtcgcc accaaggccc ggacaaccgg cgcagtcatc gccggcgaag gcaacggcgg
    62461 ggtgatctat ccggccctcc acctcggccg cgacgccatc gtcggcatcg ccctggtctt
    62521 gcaatcgctg gcggagaagg agaaatcact cgccgaactt cagtccgact tgccgcagta
    62581 tgtcatggtc aaggacaaga tcgccctccc cttcgacgcc gacgccaagg ccatggtggc
    62641 ccgcctgatc cagaaacacg ccggcgacaa ggtcgacttg accgatggtc tcaaatttct
    62701 ctacgatcac gcctgggtcc acatccgggc ctccaacacc gaaccgatca tccgcgtcat
    62761 cgccgaggcc cgcagcgaag aagaggcgcg ccggcgcgtg gacgccctca aacaggaatt
    62821 cgccagtttg cagtgataga agaaaacgag aggttattat ggccgatttt gaaaagcttg
    62881 ggatgtttta tctcggcaag gaatatgaca cggagaagaa gcagcttctc gacagccaca
    62941 ccctgtacga ctccaaggac ctggtcacgc acggcgtctg catcggcatg accggcagcg
    63001 gcaagaccgg tctctgcatc tccctgctgg aagaggccgc catcgacaat atcccggccc
    63061 tgatcgttga tcccaagggg gacatgacca atctcctcct cacctttccc ggactctcgg
    63121 cggccgaatt cgctccctgg atcaacgagg acgatgcccg ccgtgagaat aaaacccccg
    63181 aggaatatgc caccggccag gcggaactct ggaaaaaggg gttggaacag tggggcgaag
    63241 atggcgcacg cattcagcgc ctccgtgacg ccgccaattt caccatcttc actcccggca
    63301 gcaccgccgg cgtcccgatc tctatcctga gcagtttcgc ctgcccttcg gaggagacca
    63361 tgctcgaccg cgaagcggtc ggcgatctca tctcctccac cgtgaccagt ttgctcggcc
    63421 ttatcggcat cgctgcggat ccggtcaaaa gccgggagca cattctactc tccaacatta
    63481 tcgatcactc ctggcagcag ggcaaggagc tagacctggc cggcctgatc accgccattc
    63541 aaaatccccc attcgagagg gtcggcgcct ttgccgtgga tgccttttat cccgccaagg
    63601 agcgttttga actcgccatc agcctcaaca acctcctcgc cgcaccgggc ttttccacct
    63661 ggttgagcgg cgagcccttg gacatcagcc gcctgctcta taccgagagc ggcaagccgc
    63721 gccactcgat cttttacatc gcccacctca gcgattccga gcggatgttc ttcgtcaccc
    63781 tgctctttaa ccagatggtg tcgtggatgc gcagccagcc cggcaccacc agcctgcgcg
    63841 ccctcctcta catggacgaa gtggccggct acctgccgcc agtggcggcg cccccgtcca
    63901 aaaagccgct gatgatcatg cttaaacagg cccgcgcctt cggcgtcggg gtcctcctgg
    63961 ccacgcagaa cccggtcgac ctcgattaca aggctctttc caataccggc acctggttca
    64021 tcggccgtct gcagacccag caggatatcg aaaaggtggt ggaggggctg cgctccggcg
    64081 ccggcgacct cgatccccgc atcacccgcg ccctctccgc cctgggcaag cgggtctttc
    64141 tgatgaaaaa catccacgag agcgatccgg tcatttttca gacgcgctgg gccctctcct
    64201 atctgcgcgg acccctgacc ctcgcccaga tcaaaaaact gccggcgccc gccagcacag
    64261 cccaggcccg ggcggccgtc aaccagaagg ctgctggctt cgggaccgat gccgaaggaa
    64321 ggccggcgct gccgcctcag atcccggagt atttcgcccc cctgcggggc gccctcaggc
    64381 cggaggccac cctgctctac agaccggggg tgctcggact tgccgacgtc gcctttgtgc
    64441 cgggcgaatc ccaacgcttc ggacgcatcg cctggctcga ggagggcccg atgccggtcg
    64501 attgggaaca ggccgtcgaa gcccccttct ccgaggacga tctcgaaagg aatcccgaag
    64561 aaaacggcgc ttttgccaaa ctcgccgctc cggcctcccg gcccgagagc taccccgcct
    64621 gggctaaaag cttcgccgat gccatctatc gcacctccaa gctggaactc tacaccagca
    64681 cctcgctcaa gttaacctcc caaccgggcg agtcagagcg cgattttcgc atccgcatca
    64741 gtcagcttgc ccgggaaaaa cgcgatgacg ctatcgacca gctgcgcaag cgctatgcca
    64801 ccaagtcggc gacactgcag gaccgcaaaa tgcgcgcaga acaaaaggtg gccaaagaga
    64861 agaacgatta tcactcccag accatgtcga ccgcggtatc ggtgggggcg accatcctcg
    64921 gcgccttctt cggccgcaaa aagctctccg ccaccaccct gaacaaggcg ggtacggtca
    64981 tccgcaaggg catgcgtaca acccaggaac gcagcgatat ccagaacgcc gaggagagcc
    65041 ttgaggtcat caaccagcgc ctggcggatc tggaacaaga gctcaatcag gagatcgccc
    65101 gcatcgagga acagaccgat cccatgcagc agcccctcga ggtggtggcc atgcgtccga
    65161 aaaagggtga tatcaacgtc cgtctcaccg ccttgatctg gctgccctat tggcagacaa
    65221 acgatggcaa gagggagccc tcttacctgt agagaaacgg cgccagcggc actggccggg
    65281 ccgccctctc tcaaggaagg aaagaccatg cagaatatca aagatctctc cgggatgacc
    65341 tacaaaatca tcgaccacac cgccgacgtc ggactcgaac tggaggccgc gaccctgtcc
    65401 gggctcttta ccgctgcggc ggatggtctg atgcatatca tctgcccccg atgccttatt
    65461 cagcccgtcc agagccatgc cctgcaggtt tgcggagaaa accttgaaga actgctggtc
    65521 aactggctct ccgagttgaa ctacctttgc caggtgcagc actttctcac cgccagggtt
    65581 cagatcaggg agatccagga gcgctttctg caggccgaag tgacaggcga gagaagcgac
    65641 cccctccgtc acgctgtgcg cagtgagatc aaggccgtaa cctaccaccg gatcgaggtt
    65701 catcaggagg cggcggataa gtggcgtggc cgcatctact tcgatatctg agcgcagaca
    65761 tacccggccg ctccgtcaca acaggagcgt aaccatgtca acccccattg atcgcatcac
    65821 cgattatctc ttcgaaatcc cccaaaccgg caagatgcgg gtccccgccc gcatctatgc
    65881 caccgcaaac atgctccccg atatcctcaa ggaccgcgcc gccgaacagg ccgcccaggt
    65941 cgcctggctg ccgggcatcg tgggctattc ctatgccatg cccgacgtcc attggggcta
    66001 cggctttccc atcggcggcg tcgccgcctt tgatatggat aaggggatca tctcccccgg
    66061 cggggtgggc tacgacatta actgcggcgt ccgcctgctg cgaaccggcc tccagcgcag
    66121 cgagatcgaa tcgcggctgc ggccggctgt ggaagccctc ttccgtgcca tcccgagcgg
    66181 agtgggatcc accggcaagc tgaagctgaa cgagaaggag gaaaaacggg tcctggaaaa
    66241 tggcgcactc tgggctgtgc gaaatggcta cggcggcgag gaggatctgg acaagatcga
    66301 ggaaaacggg aggatggccg gagccgaccc ggaccagatc agcgcacgag cacgcgaaag
    66361 ggggcgcagc cagctcggga ccctgggttc gggcaaccat tttatcgaac tgggggtggt
    66421 cgatgagatc tatgatcccc gggccgcggc ggccatggga ctaaggcttg accaaatcac
    66481 cctcttcatc cataccggct cgcgcggcct cggccatcag gtctgcgatg atgccatcaa
    66541 ggagatgctg catgccgcgc agaaatacgg catcgagctg ccggaccggc agctttgctg
    66601 tgcgcctatc tcctcacctg aaggccagcg ctacctggcg gcgatgtccg ccgcagtcaa
    66661 tttcgccttt gccaaccgcc agatgatcac ccattgggcg cgtgaagccc tgatgcaggc
    66721 ctggagcatc tctccgcgcg acctggacct gcaggtcgtt tacgaggtcg cgcataacat
    66781 cgccaaggtc gaaaggcaca ccgtcgccgg tgtcgagcgc cccctttgcg tccaccgcaa
    66841 gggggcgacc cgggccttcg gtccccatca tcccgaaatc cctgcagcct atcgggagat
    66901 cggccagcct gtgctcatcc ccggggatat ggggcgctgc tcctatctgc tgaccggtac
    66961 aaccagggcg atgagtgaca gctttggatc agcctgccac ggcgctggcc gggtgatgag
    67021 ccgaacccag gcggtcaaat cggcacgggg gcgttcggtg gtgcgcgagc tggcggagcg
    67081 cggcatcctg gtccgcgccg aaagcagtga aaccatcgcc gaagagattc ccgaagccta
    67141 caagaacgtc actcaggtag tcgacgccgt cgccggcgcc ggcctggcct gcaaggtggc
    67201 taaattaaaa cccctgggcg tcatcaaggg ctgatgcgtt cagggcggaa agagcacgga
    67261 gggattgggt gcgcagcgtc agaagcaggt ggaaacgaga tttcggggga ggggagacag
    67321 cgccagatgt cagatgggaa ccagctttcg gggtactccc tggcggaaat agctgttgac
    67381 ttttaatgta cagcatttta tattttaaca caccccgttg ttatcgccgt ggctcgtata
    67441 atcttgcagt tccggcttgc ggccgattct atatattatc ttctcacttg tgtcaggcag
    67501 aacgaaagat cttgggtaag cctgaattcc tcaaaattcc agtagacccg tcggtacaat
    67561 ccatatcgaa cacttttcca ttcctcgcca ccgcgccttc ccgctctgtc cgcgccgcca
    67621 ggtgaagagg aagccagcgg ctgtatcccg caagctgcac aaggagtctt gagcatggcc
    67681 tttccatctt caactgctag ccccctgcag tccctcacgg acaagatcgg cggattttgc
    67741 ggatatgaaa aatccgccac cagggaacca tccgatctca acctgcgccg cttcctcatc
    67801 gccggaatcg atgcccgcct gaaggatctc gaggagatcc cgtcggccgc gatcgaggag
    67861 gatcagcgtc tcctcctgga ggtcaccaaa agcacccggc gcaagctcat caccatcagc
    67921 gaaagcctga gcgcccccac ctacgccggt caggactttt tcacccggga ccacattgct
    67981 gaaaagagag tggctggcct ttacaccctc gaaaagtcca tgcttgacga acttgccgac
    68041 atccagcttg aactcgcggc ctttcaggcc ggtcccatcg acaagatcac gatcgaagac
    68101 cattttctcc atatctccga ttcgatcgat aatctgaacc aggccctttt tgaacgtgag
    68161 tgcctgattc ttggcgatca gtagttcgtg taaagttaat cccgcggccg atgagcaggc
    68221 taggccggaa cgcagaagga tttcagggga tcggcatcag cagggggtgc ggatcaggag
    68281 cgttcattca agatcgctca atccatattc gttcatcttg cggtagaggg tggcttcgcc
    68341 aatcttgagg gcggcggcgg tcctgccgcg gtgatagtgg ttgcggatca gctgttcgcg
    68401 gatgaactcg cgctcatact gttccatggc gctcctgagg ctgtccgctt cccgggggcg
    68461 cctggctgaa gcctcgaccg gctgcatgtc ccccggcaag tcctccatgg cgatcacctc
    68521 ccccgtggtg aatatcatca gtctttcaat gaaattctcc agctcgcgca cctctccctt
    68581 ccattcccgt ttgacaatca tctccaatac cccgggatcc gcccccctga catgacggtt
    68641 catctgccgg ttgtatttct ggatgaaatg gtccaccagc aaagggatgt cgctgggcct
    68701 ctcccgcagg gaggggatat gaatttcgat gacattcagg cggaagtaga ggtcttcgcg
    68761 gaaccggccg gcctggacct cctgccagag attgtggttg gtggcggcga tggtgcggac
    68821 gttgacagga aggacggcgg tcgccccgac cgggaggatc tcccggtttt ccagggcgcg
    68881 cagcagcttg gcttgcccgg tgagcgacat atcgccgatt tcgtcgagga aaagagtccc
    68941 gttgtgcgca accttgaaca ggccgtcctt gtccactacc gcccccgtga agctgccacg
    69001 cttgtgaccg aaaagttcgc tctccatcag ggtttcggtg atggcgccgc agttgatggc
    69061 cacgaaacga cccttgcgcc tggggctgtt gtaatgaatc gccttggcca ccagctcctt
    69121 gcccgtgcca ctgttgccgg taatcaggac atggctgtcg ctgtcggaaa ccttgcgaat
    69181 ggtggcgaag agctttctga tcgccggact cttgccgatg atgttgctga aatcgtactg
    69241 ggcattgatc tcctgacgca gggcctggtt ttcctgcacc agctccttgt gctcgagcag
    69301 ctttttgacc ttgatcgcca catcctcaaa attgaggggc ttgagcagat agtcataggc
    69361 tcccttgcgc agggcctcca gggcggtctc gagagaggca taggccgtga tgatgatgaa
    69421 ggaagcgcgc aggttcagtt cggcgctttt ttccatcagt tcgatcccgg tcatcaaggg
    69481 catggaaata tcggtgacca cgacgtcata ctccccggcg ccgagcatct ccagggcctg
    69541 ttgtccgttg ctggcctggt cacaggcgaa gccgcggctg accagcatgg agaccagaat
    69601 ctcacgggtg atgtcgtcat cttccacgat gagcacacgc tgtgccatga cctcagactc
    69661 cttcctcttc actgagagcc ggcaggatga tctccacgca tgttcccctc cccggctcgc
    69721 tcgcaaggct gattgtaccg ccatgggccg ttataatggc ccggctgata aacagcccca
    69781 gaccgctgcc cttgctcccc ctggtggtga accagggggt gaaaagatgg ggctgatccg
    69841 ggagggggat ccccgttccg ctgtccgtca ccgacagatg gacctggtct ccttcatgcc
    69901 aggacctgag ctgcagtgag ccggccggca cggcctcgag ggcatcggcg gcattaagac
    69961 agagagcgat gataacctgc agcagctggt ccgggctggc cgagacctgt gggatggctg
    70021 cctccagctc ggtgcccacc tcgcgatact tgaggcggcg gtcgtacttg acgatgcgca
    70081 ccgcctccat gatcaggctg ttgaggtaga ccttctcccg gagggtgcac agcggccggg
    70141 aaaaatcgac gattgtacgc agaatctgcg tcagccggtc caccgcctgc ccgatctgtt
    70201 tcaggttctg ctccaccagg ggatctgtgc tcttcgcact gagaaactgg gctatcgaag
    70261 agatcgccat caggggcgtg ccggcatcat ggatgaatcc gggcgcaagc atgctcatgg
    70321 catgcatctt ttccgcctgc agcagataat cacgcaatgg cgtcctgtcc agctccccgg
    70381 ctgcggtctc tttctcatca gacattactg aactcccttg caaactcggt cgctttttgc
    70441 cggtgctttc gaacaagtta acaaaaagag ctctgattcg caattgaaaa tcccccggtc
    70501 tgctgccgtc tccgcatgtt ttaccttgca taaaatacat tattttaata ttttcaagct
    70561 gtattcagat caggagaaaa gcacatgatc gccagaaact ttgcccagga aaaacttgcc
    70621 gaatctacca tcaaggaact cgggacactc gcccgccagt gccgcggcga tattcttaaa
    70681 atgaccaccc tcgcggccag cggtcatccc ggcggcagca tgtcctccat cgatttttac
    70741 ctcaccgtct tcgccatggc caacgtcgat ccctgcaatc ccttcgcccc gggacgcgac
    70801 cgcatcgtca ccagtcacgg ccacacctct ccgggcgtct tcgccgcact cggacgcctc
    70861 ggctttttcg cgatcgaaga cgcggtaacc catttccgtc agaccaactc gccctttgag
    70921 ggccatgtcg aacgcgaggt acccggggtc gagtggggca ccggcaatct cggtcagggc
    70981 ctcagcgccc tgcttggcct cggcctcgga gccagactgc agggccataa catccagctc
    71041 tacggtttta tgggcgacgg agaacagcag aagggccaga tcgcagaagc ccgccgcttc
    71101 gccgtcaagt acggcatgac caacgccacg gtttttgtcg actataacca actccagatc
    71161 agcggtccga tcgccaaggt gatgccacag cagatccgcg cgggctggga agccgacggc
    71221 tggaaggtct tggaaattga cggccacgat taccaggcga tctaccaggc gatccgtcag
    71281 gcccacagcc tgcctgcccc ggtgatgatt ctcgcccaca ccgtaatggg caagggagtg
    71341 ccctccatgg aaaacgacca caagtggcac ggacaagccc tgccgattga ggagtgccgt
    71401 aaagccctgg ccctgctcgg cctgacggat gatctcgacg ctctcgtgga acgccgaaaa
    71461 acgcacaatc cagctgtccc gctcctccgg agagagcacc tctcggctgt tgccgatcca
    71521 ggcaccccgc gcacctatgg cgccgctgaa aaaaatgaca accgcagcgg ctggggaaac
    71581 gccctcgaag acatcgtcgc cgccaaccat ggccgcctgc cgatcgcggt tttcgactgc
    71641 gacctcgccc cctcggtcaa gaccaccgcc atcgccaaaa attacccgga tattttcttt
    71701 cagggcggca tccaggagca taacaccgcc gccctcgccg gggcctgttc ggccgaaggg
    71761 gtgctcacct actgggccga tttcggggtc ttcggggtcg ctgaaacctt caaccagcat
    71821 cgcctcaacg atatcaacgg cactaactac aaggtggtct gcacccacgt cggcctcgat
    71881 gtcggcgagg atggcaagac ccatcaatgc atcgattacg tcggagcttt tcagaatctc
    71941 ttcggtttta aggtcatcgt gccggccgat cccaatcaga ccgaccgggc cacccgctac
    72001 atctccgcca ccccgggcaa ttttctcctc gccatgggac gctccaagat gggcatggtg
    72061 ctcgacgaga cgggagagcc cttttacggc ggcagttact cttttgtcta cggcaaggcc
    72121 gatctgatcc gcagcggcgg catgggcgcc atcatcgcca tgggcgggat ggtcggccgc
    72181 gcagtcaagg cctgggaact gctcaaggcg gagggcatcg aggtcatggt gctctcgatg
    72241 gcctgcccat cggatcccga catggaggcc ctcgcccgag cagcgaaaac cggactgatc
    72301 gtgacttacg aagaccatca tgtccgcacc ggcctcggca gcgccgtcgc caatgccctg
    72361 gctgatgccg gcctggccac ccgcttgatc aaactgggca tcgcccgcta cggcagctcg
    72421 ggcattcccg acgaactcta ccgcagccag ggcctcgatc ccgaaaacct cgcccgcacc
    72481 ttcacgtccg ctctcaagcg ctagaacctt cccccttgcc ctgaacaaga aagcctctcc
    72541 ggtctatgga ccggagaggc tttctgcttg atgggccggg gcagctccag ggctgcggct
    72601 cacgccttta ttctgattag cgactggtca tagtcggccg gggcctcata tcccaaaaga
    72661 ttgaacagcg tggcggcgat gttgctcaaa ccctgattgg ccagcccggc caactcgtac
    72721 tccccggcat attccggatc gacgatgaca aaggggaccg gattgagggt gtgggcggtc
    72781 ttgaccgact tgacgccctt cttcactgtg aacatctcgt cggcattgcc gtggtcggcg
    72841 gtgacgacgg caatacccct gagctcacga accagctgca gcagcactcc gacgcactcg
    72901 tcaacggtct ccaccgcctc gatcgccgcc tccatgattc cggtgtgacc gaccatgtcg
    72961 ccgttggcaa agttgacccg gccgaagcgg aaccgcccgc tgcgcagaag tgccccggcc
    73021 tgctcagcga tctcaacggc cttcatcttc ggggccttgt cgaactgaat cctgtccgag
    73081 gggatctcga caaatttttc cagcttttca tcgatataac cggagcgatt gccgttccag
    73141 aaataggtca catgcccgta cttctgggtc tccgaaagag caaaggaggt gaccccggcc
    73201 gcgcaaagat attcagaaag gacgcgatcg atttcaggcg gcgaaaccag gtatttgtgc
    73261 gggatgtgca ggtcgccgtc atactccatc attccggcat agagcacatc ggggctgcgc
    73321 tcgcggttaa aagcattaaa atcccgctca tcaaaagcgc gcgagatctc gatggcgcga
    73381 tcaccacgaa aattgaaatt caccacgcca tcgccgtcct ggatggtccc aaccggtttt
    73441 cctgcggcat cgacgatgac aaaggcgggg aggtactgat ctgtcgcctc ggcatcctca
    73501 gcgtacatgc tctccactgc ctcggcggcc gaggagaagc cccgggcccg gccgaggacg
    73561 tgggcatccc agccgcgctt gaccactgtc cagtcggcgt tatagcggtc catcgtcgtc
    73621 accatgcgcc cgcctcccga agcgatgcga aaatcgcatc cccgctcctg gttgaggcgg
    73681 gtgagcagct cttcggtctg ttggatatag gtgagcgcag tcctggcccc gacatcgcgg
    73741 ccatcgagga gtatatgcag gcgcacccgc cgcacaccct cttccacgca gttttcaagc
    73801 agggcaaaga gctgtctgat attggagtga acattcccgt ccgagagcag gccgatgaaa
    73861 tgcagggttt tcccgctctt gccgcgaccg accaactcct gccagatccc ggtggtgaag
    73921 atcttcccgc tggctatcga gccgtccacg agcttggctc cctgggcgaa gacgcgcccg
    73981 gcaccgattg cgttgtggcc cacctcgctg ttgcccatat cctcatcggt gggcaggcca
    74041 acggcggtgc cgtgagcctt gagacgggtg aaaaaacggc tgcgcaacag ttcgtccaga
    74101 accggcgtcc tggccataaa gaccgcattg gtttcgtctt cccggcccag gccgacgcca
    74161 tccataatga tgagcagcag ggggccgggc cggccggaaa aatgggcgag ctttttcagt
    74221 tgcagatcca tatgatcaac ctttcaaaat catgtccatt gccgtgatgc acagtaaaat
    74281 ataaaatagt aaaaagcggg ataaaataaa agcccttctt ctcgcgccgg gcgccaccct
    74341 caacaaaata attgatataa ttcccaaaat agtttatctt ggtgcgacca aaaccatcat
    74401 cagctagccg ggatcgacct catgacctac aaagatatcc ttccccttgt ccataaaaat
    74461 gacgaaaaaa tcctgctgat cctgctcggc ggcgttggcg gcatccaatc cggccccaat
    74521 ctgctcaccg aactgcagca ggcgcatcgt ccccacctca acgccctggc gcgcaaatcg
    74581 gcctgcggtt tgcttttgcc ggtcgctccg ggcgtgaccc ctgccgccga tgctgccctg
    74641 agacgcatgc tcggctggga agaggagcag gccccgacct ggacgagcgg gctcgggctt
    74701 gcaagctgcg ccctctccgc ctctccggag tatctcgctc tcttcacaca agccggcatc
    74761 aacgccatcg cttcgcccga cgagccggca gggttgatcg accgggcctg cgccgaaatc
    74821 ttgaaacacg acatggtcat cctccactat gccgcccccg aaaaagaagg gctggccggc
    74881 ggttattatg agaaaatcaa ggccatcgag gatctggatc gtttgatccc gcagcttctg
    74941 gaatccgcac cggccgtcgt cgccgtctgc ggcgaccact cctgcccgag tgccctgggc
    75001 gcgatctcct ggcaccccgt gcccctttta atccatgccc ttacggcacg ttatgacatg
    75061 gtccaaacct acgatgaaat cgcctgtacc gccggtgccc ttggcacgct ccgcagtgcc
    75121 gagctgctcc ccctcctgct cgcccatgca ggaaaactgg gcccggcaca ctagagaggt
    75181 aagtatggtt aaaagccccg cgattccacc cgatcccggc agcctttccc ccgctgtcat
    75241 ggccggcctt atcgaccaca ccctcctcaa acccgaggct gtggccgccc ggttcgaaga
    75301 actctgcgac gaagcggccc gctatcgctt cgccactgtc tgcgtgaatc ctgcctgggt
    75361 gcgcttttgc gccgaacgcc tccagggttc gggtatcggc ctttgctcgg tcgtcggttt
    75421 tccgcttggc aacatcacgc cggaagacaa ggcggcggag gcccgcaggg cggtcggcaa
    75481 cggcgccacc gagattgaca tggtactcca cgtcggctgg ctgaaatcgg gatttgcttc
    75541 gttggtgctg gaggatatcg cccgggtggt ggaggcgtcc gcaccccatg cggtcaaggt
    75601 gattctcgag acctgcctgc tcacggagga ggaaaagatc gccgcctgcg agctcgcccg
    75661 atcggccgga gcccattttg tcaagacttc gacgggattc ggcaagggcg gagccacggt
    75721 ggaagacatt gccctcatgc gccgctgcgt cggggaggcc ctgggcgtca aggcctcggg
    75781 cgggattcgt gatctgccaa ccgcactggc gatgatcgcc gccggggcca atcgcattgg
    75841 caccagctcc ggagtgacca tcatcgagga gataaagcaa cttaactctt aaaaaaaaag
    75901 ccctcaaacg agggcttttt ccagattggc aagtagctgg gggctgaccg ccaccccctc
    75961 cttttgtagc cccatgatgc aggagccgat ggcgggctcg aagcggggct cgataaactc
    76021 cgcactccgc accaccttca gggcctcctc gcgcatgaag ggcataagca catcgcgctg
    76081 attgaagatg ctgccgatga gtgcgacggc gacctccccc ccttcacggc ccatgcgttt
    76141 ggcggccccg gcgaccagtc ggccgatctc cctgccggcg ctttcaatga tctgacgcgc
    76201 tgcagcatcc ccctcctgcg cggccgcaaa aaccgtcggc gcattgctgg cgatctcggt
    76261 acggtcaatc ttgtcggagt agatctgcga gatgatcagg tcaatgctgg ccagatggta
    76321 gcgtttttca taatggcccc gcagcacggt ttccggcccg cgcccatcca gatccttcaa
    76381 tgccgccatc agcgcctgct gaccgatgta atagccgctc ccctcgtcgc ccagcttgaa
    76441 gccccatccg ccgcagcggt agagcgtgcc cttttcatcc ttgccgaagc agatggatcc
    76501 cgttccggag atgacgatga tgcccggtcc gccgccaaag gcccccgcga gggcagccga
    76561 ggcgtcggta tcgatggtat gagccctcgc caggccgcgg ctggtgacct gggccgaaat
    76621 cgcctcgcga tccgccggac gtccggcgcc cgccaggccg gcgtagagat gatcgacctc
    76681 ctcctgcctg cagccggcga tggcgaccag ttcggcgatc agtccggcca ggctctcacc
    76741 caaacgctcc gcacctatgg cctgaatatt ggaggagtcg ccggtggcgc gcgccaacag
    76801 ccggccgttt tcgtcaatca taacacccag ggttttggtg ccgccgccat ccaggccgat
    76861 aatccgcttt gccatgctct cttctcctct atgatctcaa aaaggattga aattttcttg
    76921 ccaccaggag ggccgccgca accttgacgc actctcccgg gatgaacaga agcagtccgg
    76981 cccctattgc cttgagaaag gggatcggcc tgccggtgat ccatgcaaag gaaaaataca
    77041 accataaagt cccaaagaca agtaaaaaaa gcgcgcccag ggccatcacc agcgccaagg
    77101 agcgcattgc cgggggccgg gccaacgatt gcacaccccg ggcgaccagg gccgccgtca
    77161 ggggcatggc gagaagatag ccaaaggtgg gctggaagag gtaggccatg ccgccgccct
    77221 ggctgaaaag cggcagcccc agaagtccca acatcagata ggccgcctgg gtcccggctg
    77281 ctgcccgggg cgagaagagc gcaccggcca gcagggggaa gaggctctgc agcgaaatcg
    77341 gcaccggata aaagggtatc cgcagcagcg atccgaaagc ggtgagcgct gtcatcaggg
    77401 ccattgcact cagtgtccgc gtcgtcatcc tcatcaatag actcctcccg tacaaacgaa
    77461 ccggactgtc tcccgggtat tccaggacgc ctcccccttc gtccggcacc acgccaggcg
    77521 cagccgttgt tgcattcctt gtgctcccat tttgctgttg cttttgcctc cccgctttga
    77581 tatattatcc attggagata ttttgacgat gaaggtgcag cgcctcttcc ctgtaaagtg
    77641 aggggaaaag ccttgcacct tgtttattaa tggccgcctc tccttaaacc gacggctcca
    77701 atagcaaact cccatccacc tagatgcgca tggaaatccg attaacgcac ccagcgatcc
    77761 ggcgatccac tgcagcggcc gcactcagga gccgttggct ccttctcgtc ctcttcttcg
    77821 ccgccgcgac agccgcctgg ccacagcgcc tgctcaccat cccgctgcct cgcgaagccg
    77881 tccaggcccc cttttctctg gtccccgagg agagggtggc cggccccacc gtgggtctgg
    77941 ccctcagcgg cggcggcctg cgcggaatcg cccacgtcgg ggttctccag accctgagcg
    78001 aacacaacat ccccatcgat tatatcagcg gaaccagcat cggatccatc atcggcgggc
    78061 tctatgcctc aggctattca cccgaggaac tctggaaaat catcgaaaaa ctcgacctcg
    78121 gggatctgct caatgactct ccgagccggg ccaatcagtt tctcgcggaa aaacagaaac
    78181 agagccgggc ctttctccaa ctccggatgg cgaattggcg tttctacctg ccggaggcga
    78241 tcaccccggg ccagaaactg accaatatcc tgaccgatct ctatctcaac gcccccctgc
    78301 acgaatcgga tttcagctgc ctgcggatgc ccctcaagat ccagaccacc gatctactca
    78361 ccggcagcaa aatcgtgctg cagcagggca acctgatcga ggcgatgcgc gcctcgatcg
    78421 cggtgccgct gctgcttctg cccgtggagt acaacaactc cctgctcgtc gatggcggcc
    78481 tgctcgacaa cctccccgtc gaggaaaccc gggcaatggg agcggatatc gtcatcgccg
    78541 tcgactgcac cgccaatttg cgcgcacgcg cagagatgga acttccctgg gagctgatgg
    78601 accaggtaac caccatcatg cagcagggcc acaatctcga acagctcgag aaggccgatc
    78661 tgatcctcag ctttaagtct tttccggcca gcacaaccca gcacgaggtg gtcaggcagc
    78721 tctatgagac cgcgcgagca cagaccgccg agatcatccc gcagctgaaa accctgcttg
    78781 aaaaaaggag agctgaagag cggtccgccc tgaacagccc cttgttcagg gtagagcggg
    78841 tctctttttc gggctcctca caccttggat tcaccgatct catcgacgcc cgctcgggcc
    78901 ggccctatac cccggccgac atccaggcca atctggccac cctctacgaa accggctatt
    78961 tcgagcaggt tgaagcacat ctcgatgcgg ccggcggcga caccaccctg gtctacgatt
    79021 gcatgcccta tccggagctg aaagagatcg atctgcaagg aaactccgtg ctcgtggaca
    79081 gtgttctact caaccccttt gctcccctgg tggggcaccc tctcaaccgg catcaggcgc
    79141 gggaggccct gcagcagatc atccggctct accgtgaaga gggctattcc ctcgcctcgc
    79201 tgcgtgcggt gcacttcgac cgggagaggg gcatcgcccg gatcaccatc gacgagggcc
    79261 ggatcgcccg cgttcgtttc gagggccagg agatgaccag aaattcggtc ctcaaccgcg
    79321 atttttccct caggagcgga gacctctttc aacgccgaca ggcccagcag gcggtcaaca
    79381 atctttacgg caccggtctt ttcaactccg tcacccttct cccccaggcc ggcccggagg
    79441 gatgggagct gatcgtccgc ctgcatgaaa agaaatacac catcctccgc ctgggcagcc
    79501 gctacgatct ggaacggaac ggccgggcct ttctggaggt ggcccatgaa aacacctttg
    79561 gcagcggcaa cgacatcatc ctgcacggcc aatacggaga ccgcgaccac aagctttttc
    79621 tccagtacaa cgccgaccgc ctggcgaact ctgatctgag ttttcaggcc acggcccggc
    79681 aggagtatgc caagcgtttc tattataaaa acctcaccgg tgtgggtgaa tatgaacgcc
    79741 gcggcaatgg ggcgcatatc agtctgggca tgcagatcgc ccgcctcggc atgctctccg
    79801 gctttatacg gctggagaag atgaacatcc accgcctctc cggcagcatc atcgatgccg
    79861 gcgagctgaa cttgaacacc atcggactca acagtgtcgt cgatacccgc gaccagatcc
    79921 cctttccaac gcgcggaaaa tatcacacct tcacctatga ggtctcctcc ggaaaatttc
    79981 tcggtgcgga catctccttt ttcaaggtac aaaaccagct caccacatac tggaccttca
    80041 gaaaccgcaa taccttctcg ccccggcttc tctggggcac ctcggatctg acgacgccat
    80101 tctcggaaca ttacaggatc ggcggggagg actctttcta tggattgcgc gaggaggagt
    80161 ggagcggaag gaatatggtg ttgggcagcc tcgaataccg ctactgcctg ccctggaaga
    80221 gctatttgac cttttatctt tcagcgcgct tcgatttcgg cgccgtctgg gaaaactccg
    80281 tcgaggtgaa aggcaacgat tttatctccg gccggggagc cgccctgagc ctcaaaacac
    80341 cccttggacc cttttcagcc gcctacggcc gcgccagcaa cggccacgac cgcttttact
    80401 tctccgccgg ctataacttt tgatcgcgct cccggttcca gttcacccag tcgctctgct
    80461 cttcgggatc ctctcggctg aaagagccgc tctggacgac ctcgcccctg gcgtcgacac
    80521 gcacccgctg catgctccgg acgatcaggc gccactcctc cagcgaaacc tcgcgcggcg
    80581 gcgcaacctc ggtcggtccc tgcacctgct gcggggctga ggaaagcccc ttgcgcaggc
    80641 tgtcccctgc tgtgacccgg ctcagcacca cctcccccga atagacccgg agttcagcgc
    80701 aggagtcggc gtcgacgctg aggcgcaggg tggtaccggt gatggcggct gctgcgaccg
    80761 gcgtagagat cgaaaaacgc tgcccgccgc tctttttacc gacatgggcc cacaactcgc
    80821 cggattggag ctgaatggct gattcgcgct ggtgctccac agactcttcg tagagcttaa
    80881 ggatgtcaat ggtggtgcgg ggcgccatgc gcacgcgatc gagacgggcg atctccagtt
    80941 ccgcacggga ctcggtgaag gtcctgaccc tttcgcctga aatcaccgtc gaatccacca
    81001 gcacacggtg ccactccgcc tctgttcccc tctgcttctt ggccgatccc tcaacaaagg
    81061 tcacgacgcc cttgcgggcc gcttcttcag catcgggggg caatcccgcc gccagtaaaa
    81121 cagccgcagc ggccagagcc gcaatcgccg ctccaaccgc gcgcactctc ctgtaccaca
    81181 tgatcttccc tcccgcagtg aagggatttg ctctcttccg ctcccggtca ggagcgcttc
    81241 ttgccttggg gatcgggcga cggaaatatg gagacgatct catcgccctt gacgacattg
    81301 accacagccg cgccctgggt cgcgcggccg ctcaatttga tctccttggc cttgatccgc
    81361 ttcagcttgc cctttttggt caccacgatg aattgatcca tcgctccggc ttccgccaca
    81421 gccgcgatcc agcctgattt ctccgagcat ttataattaa cgatgccctt gccgccgcgt
    81481 ttgatcgtgc catactccga ggcttcgctg cgcttgccaa atcccgcatg ggtgatgctc
    81541 agcaccgccg ccttgtcccg caccagcgcc accaggctca ccacgcgatc atccgcctcc
    81601 agctcgatac ccttgatgcc cccggccgcc gctcccatgt cgcgcacatc ctcctcggag
    81661 aagcggatgc tcttgccctg gcgcgtggcc aggagcagtt ccgtcgcgcc gtcggtcatc
    81721 tccgcgccgg cgagggcatc atcctcgcgc agaccgatca ccatctgccc gccctctttg
    81781 atgcgctgca gttcggagaa attgaccttc ttgaccagcc cctggcgggt ggccacaaag
    81841 agaaagcgtt cgctgctctc cttgggcatc tcgaagcagg cgatgacctc ctcttcgctc
    81901 ttgaaatgga gaagccggga gatctcgcct cccttgacct cgtccgaagc aaaggggatg
    81961 aagctggtcc gcgagagata ggcccgcccc gaacggcaga caaagaccag cagttggctg
    82021 ctgaccgaac ggaaggcaaa gcggagaaaa tccttttcac cgagcagagc ggagaccgtc
    82081 tcgctgccat aatcctgggg gctgaatcgg ccgatgcgcc cccgggcagt cagggccagg
    82141 atgaactcct cgtccgagac catctcctgc agggattgtt cgccgtctcc gctgcgggtt
    82201 atgatctggg tgcgccggtt atcgccgtgc ctctccttga gatcgagcag ttcctgcttg
    82261 atcagctgca tgcgcaggga cttgctgccg atgagggatt tgagccgctg gatgagcttg
    82321 ataagctctt tatactcgtc ctcgatcttt ttacgttcaa gaccggtcag ccgctgcagg
    82381 cgcatgtcca gaatcgcctg ggcctgaatc tcggaaagtt tgaaattctt catcagtccc
    82441 ttgcgggcgg tgtccacatc cctcgacttt ttgatcagct cgatcacggc gtcgatgtta
    82501 tcgagggcga tcttgaggcc ttcaaggatg tgcgcacgag cctcggcctt ctcgagctcg
    82561 tatttgcagc ggcgcagaac aacatcatgg cggaatttga gaaactcggt gaggatctgc
    82621 ttgatgttca gcaccaccgg ctgaccgtcg acgagagcga tcatgatggc gccgaaggtg
    82681 gtctgcatct gggtatagcg aaagagatcc ttgagcacac cctcggcatc ggcctccttg
    82741 cgcagctcga aaacgatgcg catgccgtcg cggtccgatt cgtcgcgaat gtcatagatc
    82801 ccctcgatct ttttatcgcg caccatctcg gcgacgcgtt cgatcagaga ggttttgttg
    82861 acctgatagg gaatctccgt caccacgatg cgtttgcgcc cgccgctgac ctcctcgacg
    82921 tgcacccgtg cgcgcacggt gagcttgccg cggccgtgct tgaaataggc tgcgatctct
    82981 tcatccccga cgatgatggc cccggtcgga aaatcgggtc ccttgatgta cctgcgcaga
    83041 tcctcaggct tgagatccgg cttttcgatg accgccacca gggcatcgac cacctcacgg
    83101 agattgtggg gggggatgtt ggtcgccata ccgacggcaa tgcctgcggc gccgttgacc
    83161 agcagggttg gcagcagggc gggcatgacc agaggctcct tcagggagtc atcaaagttg
    83221 gggatgaaat cgacggtgtc cttgtcgatg tcccggatca cctcttcggc gatcggcgcc
    83281 aaccgcgcct cggtatagcg catggccgcg gcggcgtctc cgtcaatgga cccaaagttg
    83341 ccctggccgt ccacaagggg ataacgcagg gaaaaatcct gcaccatgcg caccatggca
    83401 tcataaaccg cggtgtcgcc gtggggatga tacttgccaa gcacctcgcc gacgatgcgc
    83461 gcgctcttct tataggccga cccgggcctc agacccagtt cgagcatgcc ataaaggacg
    83521 cggcggtgga ccggcttgag gccgtcgcgg acatccggca gggcgcgggc aacaatcacc
    83581 gacatcgaat aatcgatgta ggagtgcttc atctcctctt cgataaatac cggtacgagc
    83641 ttttcgctgt tatccgtcat ctcttcgact tttgttcctg attaaacatc cagattgcgc
    83701 acatattggg cgttctcttc aatgaatttc ctgcgcagtt cgacatcatc acccatcagc
    83761 atcgagaaga tatggtcagc ctcgaaagcc tcctcgatgg tcacctgcag cagggtgcgg
    83821 aactgcggat ccatggtcgt cttccagagc tgggccggat tcatctcgcc cagacctttg
    83881 tagcgctgga tggagtagtt tttggtctga tccttatact tctcctgcac cttgcgcagc
    83941 tcttcatcgt cgtagacata gagttcctca ttgccgcggg cgacccggta gagcggcggc
    84001 tgggcaatat agagatggcc gtgttcaatc accggacgca tgtggcggaa gaaaaaagtg
    84061 agcaggaggg tgcggatatg ggcgccgtcg acgtccgcat cggtcatgat gatgatcttg
    84121 ccgtagcgaa tgcgctccac ctgaaaatca tcccgtccga tgccggtgcc cagggtggag
    84181 acgatggtgc ggatctcttc gttggagagc atcttgtcga ggcgggcctt ttcgacattg
    84241 agaatcttgc cgcgcagggg cagaatggcc tgaaagcggc ggtctcttcc ctgtttggcg
    84301 ctgccgcctg ccgagttgcc ttcaacgatg aaaagctccg tgcgccccga ctccgaccag
    84361 gagcagtcgg ccagtttacc tgggaggctg tcattctcga gagccgattt gcgccgggtc
    84421 agctcacgcg ccttgcgcgc cgcctcgcgt gaacgggcgg ccacctggca cttttcaatg
    84481 atgcgcttgc ctacggatcc gttctgttcg agaaacttgc ccaactgctc gttgaccagg
    84541 ccctcgacga tccccttgac ctcgctgttg cccagccggg ttttggtctg cccctcgaac
    84601 tggggttcat tcaccttgac gctgatgacc gcggtcaatc cctcgcgtac atcgtccccg
    84661 ctcagggtgc tgtcgccgtt cttgagcagc ttgtttttgg tcgcgaaatt gttcagggtg
    84721 cgggtcaggg ccgccttgaa gccgaccaga tgggtgccgc cctcgatggt gtgaatgttg
    84781 ttaacgtagg agtagatatt ttcgttgtag gaatcgttgt aggagaaggc gatgtcgacg
    84841 gggatatttt cacgttctcc gctgagaaaa accggccgct tggtcagggt gttgtggccc
    84901 tggtcaagat attcgatgaa ggcgctcaga ccgcccttat atttgaattt gttgcttttc
    84961 cccgtcttga catcgaggat ggaaattttg agattgggat tgagaaaggc caactcgcgc
    85021 agccgctctg aaagaatttc aaagctgaat ttgcgttttt taaaaatttc gccgtcaggc
    85081 atgaaggtgg tcttggtgcc ggtgctcttg cggctgccga cctttttgac cggggcatcc
    85141 ggaatcccgc gctgatagcg ctggcgatag aggcctccat cccgggcgac ctcaaccacc
    85201 atccactccg agagggcatt gaccacggag acgccgacgc catggagacc gccggaaacc
    85261 ttgtaggcct ttttgtcgaa tttgccgccc gcatgcagaa cggtcatgac gacctcgagt
    85321 gcggagcggt tttgctccgg gtggatatcg acagggatgc cgcggccgtt atcggtgacc
    85381 gagacgcttt cgtcctcgtt gatggtcacc tcgatctcat cacaaaagcc gccaagggct
    85441 tcatcgatgc tgttatccac cacttcgttg accaggtggt gcagaccccg caccgagaca
    85501 tcgccgatat acatcgcggg gcgcatccgc accgcctcca ggcccttgag aacctgaatc
    85561 tgactggcgt cgtaggcagt ctgattttta acgttgttca tcgttttgaa aacacccact
    85621 attaaataaa ccggatatcc ttgaccaggc cttcgcccat ggcactgctg atgtttgcga
    85681 ggatggtctg tttctgaaag agcagttcag tgcgccaggt catcgaggcg acccgaacgt
    85741 agagcacccc atcctggaga cgctctggcc gggagatgcg gccgatcttt tctccaacca
    85801 gctgcggcca ttgatcgatc acccgctgct gtttcagacg ctgctccaga cctagctgat
    85861 gtaccagctg cgccaatccc cgcgaaatat gctggctgtc atcacgagcc attctcgccc
    85921 ctctctatcc gcccgccgcg cacaaaaaag aggtattcat ccttctgccg gtgcaggcga
    85981 tgctgcaggg acgaaaaatc agccgaggtg ccggagatga tgcactggcc gccgtcggaa
    86041 aagagctcaa tcaccttttc accccgttcc tgatcgagtt ccgcatagag gtcatccaga
    86101 aggagcagcg gcgtggtatg cagataactc ttaagcagaa aaagttcggc cgccttgatg
    86161 ctgatgagcg cactcttgtg ctcgccgcgc gaaccaaact ggcgcagggt ccgcgcgccc
    86221 acgcacaatc tgaattcatc gcgatgcggg ccgaccaggg aaactcctct ggcgatttcg
    86281 agccccttca tttttttgag acgatcgctg aagcatgcac ggggatcaga cccgggggtg
    86341 acttccacat taggctcata
//
LOCUS       DAOPWU010000002        39279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_965_length_39279_cov_5.370385, whole genome shotgun sequence.
ACCESSION   DAOPWU010000002 DAOPWU010000000
VERSION     DAOPWU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 39279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39279
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_965_length_39279_cov_5.370385"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1258
                     /locus_tag="PKM23_00375"
     CDS             26..1258
                     /locus_tag="PKM23_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY89951.1"
                     /translation="MRILIYMWEGIRIAYDALRAYKMRSILTTLGIVIGVTTVITIVA
                     LIQGLNQAFSSEISALGTDTVYIQKFPWTMQGDDWFKYRNRPNITVDEADRVKELSRT
                     VSAVAPTVETSRTIKHREKTVERVSITGTTAEYLTTANADPEIGRFFSANDVASNRPV
                     AVLGSEVAELLFGQSDPLGKRVTIGGRKFRVIGVLGKKGSFMGFNQDILAIIPIGTFQ
                     SAFGAQRRWVTIEVKAKSPQLIEETEDELTGIMRRIRKLTPEKESNFAINKQSLLLDT
                     YNQLTGTLWAVAIGVGAISLIVGGIGIMNILLVSVTERTREIGVRKALGARNSDILWQ
                     FLVESMMICSIGVLIGILMAVGIAKLVAATTPLPAAITWWVAFLGLVFVVTIGLFFGI
                     YPASKAARLNPIEALRYE"
     gene            1281..2507
                     /locus_tag="PKM23_00380"
     CDS             1281..2507
                     /locus_tag="PKM23_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY89952.1"
                     /translation="MVLTDTFHLALSAFRSNKMRSLLTLLGIIIGVMTIIAMQSLITG
                     LRKSINEQVSSLGSSVFQVQKFPAINMGPHDHDKYRNRKDITYADALAVRERVTAAKA
                     VGAEVWNWGAELRYKDRKTLPNIQLAGVTPEFLENNGLEIAEGRFITAEDVDLNVAVT
                     VIGADVVDKLFPFEDPLGKDIKLMGRRFEVVGIFEKKGSAFGQSQDNRIAIPFFQFTR
                     LYGKERSINITVQAATPALVETATEQTIGVMRTQRKVKPGIENDFEIYSNETITGFFD
                     NITKYVRIVAIAIASISLLVAGVGIMNIMLVSVTERTREIGIRKSVGAKRSDILWQFL
                     IEAIVLSELGGLIGIMLGLGIGKLVDALTPIPAAVPVWTVFLGLIFCSFVGLVFGVYP
                     ALKAARMDPITALRYE"
     gene            2535..2873
                     /locus_tag="PKM23_00385"
     CDS             2535..2873
                     /locus_tag="PKM23_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY89953.1"
                     /translation="MEIEKKVESGVLIFALEGDLMGGPEAEAFQKEILAAIEGEQVQV
                     VADLSRVRWMNSSGLGMLIRALISLRSSSGDLRLASISERLRRPMEIARLDTVFQQYD
                     AVTEAINSFN"
     gene            2884..3459
                     /locus_tag="PKM23_00390"
     CDS             2884..3459
                     /locus_tag="PKM23_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNY89954.1"
                     /translation="MIQLSREEVLDKIRSGQSLAGINMTEVDLSYESLKGVDLSGLDL
                     REAHLQNCNLEGANLSGSNLENAFLFGANLHKANLSGALLSEANLQDSNLDQAVLVKA
                     TLIHTSLFGSSLEKADLTDADLSRARMKRANLRHATLRRANLQNVHIERADLTGATLA
                     GADLTNANFTNAEIGQADFADAIREGTIGLP"
     gene            3591..4574
                     /locus_tag="PKM23_00395"
     CDS             3591..4574
                     /locus_tag="PKM23_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNY89955.1"
                     /translation="MNPQLEARLAGYKKLIQGRLEEVIRKGEPVSLYDPMRYALSQPG
                     KQLRPCLLLLSCEAVGGDPEQALDGALAIEVIHNFTLVHDDIMDHDELRRGRPTVHHR
                     WDENVAILAGDALLVLGYALLARVDSRHLPAILRTFSSAILDICEGQALDKEFETRVE
                     VSLAQYYEMIEKKTGRLFELACQAGAMLGGGEEQTSAALACYGAQVGRAFQLQDDLLD
                     LLGEEKTIGKDVASDLEEGKKSFLIAHARHTASPAQLMTLAALTERRPIEKERLTAII
                     ALFEEIGTLAAARAEISRCLEKARSNLKVLPQNPAARDLEAFIGAIAERTF"
     gene            complement(4679..5410)
                     /locus_tag="PKM23_00400"
     CDS             complement(4679..5410)
                     /locus_tag="PKM23_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89956.1"
                     /translation="MNREAEPILYYWLQSAQSMRVRRIRVVEGRERLQINLENAVFFR
                     LLRSGRPDGMSARGCATYLHFYREEARKLRAQGESKGLSTAEQRNLEEELLSYFPRAL
                     AFLKLGEYKACAQDLHHCMELARFTFRHGKGGGAAESCMEMMPTLHMIYYLALCAMSL
                     EAGHPTRALYYLDRGVQNISADYLRISQRSACSDSEEALRLRSFREIILQESRRRLAR
                     RRGKSPLHVPICQLLSQQRRPAVES"
     gene            5682..6764
                     /gene="mnmA"
                     /locus_tag="PKM23_00405"
     CDS             5682..6764
                     /gene="mnmA"
                     /locus_tag="PKM23_00405"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNY89957.1"
                     /translation="MAAHEQRVAVAMSGGVDSSVAALLLLEANYEVLGITMLHFDADS
                     EDTAPSAAEEAAMICRHLGIPHQVVDLRDRFRKSVIDGFVAEYLAGRTPNPCVLCNLT
                     IKWGALCKFALESGADLFATGHYARLVPAPPGGRHLLRRAANLDKDQSYALWRLTQEQ
                     LARTLFPLGDLTKSEVRAIATAHGLPAAHKTESQEICFIHDDNYQRFLAEELEQRGIT
                     IAEGEIITVEGKRVGRHRGYPYYTIGQRKGLGVAMGHPVFVTAIDPAANRITIGQKEQ
                     LMATGLQAQNPNWIAEAEPRRGEPVEARIRYKDPGYPALIEEAGPEGFTLRFLEPRPA
                     VTPGQSVVLYRGEIVLGGGIITRALS"
     gene            complement(6857..8395)
                     /locus_tag="PKM23_00410"
     CDS             complement(6857..8395)
                     /locus_tag="PKM23_00410"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNY89958.1"
                     /translation="MKESYALANPISVLLDKPREEFTRRDLLRVIEERQMERITLHYT
                     ALDGKLKELKIPLANRSQADHILADGERADGSSLFKGMVDASVSDLYVVPLYKTAFLN
                     PFDPNSLDFICRYLDSSGTYAAFAPDNILLNAHAYFKKSTGFTLNALGELEFYLLAEP
                     ESHIFLAGKQRGYHASAPFIKTGKVLDEMVRYITQITGAVKYAHSEVGYLENVHSDLA
                     EINGKRAEQLEIEMLPTPIDEAGDILVLAKWLIRNVAYRNNCVATFTPKLEEGVAGSG
                     MHVHVELCKDERNVMLGTDGRLTTEARRLIGGFCTYADSLTAFGNTISPAYLRLVPNQ
                     EAPTRICWSDCNRSAMIRVPLGWVNANDLGNKLNPQQDVEKESSAGRQTVELRSPDGS
                     AAVHLLLAGMTLAAEWGLTHPGSLELAEQLYVRGNIFEDPQLLQRLQALPKSCVESGK
                     LLLEKRSLYEREGIFPASIIDYSARLLFAEQDEDLNNHLLHLPADDRLKETRKLMHKD
                     LHRH"
     gene            complement(8496..9824)
                     /locus_tag="PKM23_00415"
     CDS             complement(8496..9824)
                     /locus_tag="PKM23_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY89959.1"
                     /translation="MRILLLQPAIEDFYTTPIRFYPLGLLYTAAAMREAGHEVRLVDA
                     LTPLHKRTLALPRALAHLRPYLGRSPHFFNRFYRFGREDESLLQEVRNWQPDAVGLSS
                     QFTAYYKNVHEIAGRIKAERDLLLFIGGHHATVFGAECRRRTPAIDAVCTGPAETGLQ
                     EIYDRAGAPLVKGAVDWRSLRPAHDLLPADLYRIGRSRYASLLCSRGCPNGCDFCSVD
                     RMFGRSLSYRPVEEVMSEMRFLHERRGVRIFNFEDDNLTGRMAWFREFLQGVTREPLF
                     ADIELTAMNGICYPGLDEETLGLMRRAGFKRLNLSLVTQDPGLRRRYHRPHGEGELEE
                     VIARAQRLGLFVTIYLIIGLPGQSYAEIRGTIDHLLDLGVLVGPSIFYLPPASPLYDR
                     LELPAQLLAEWELYRSSAFAVESAELPRAALIELFSYVRAENLRRRPPAA"
     gene            9940..10500
                     /locus_tag="PKM23_00420"
     CDS             9940..10500
                     /locus_tag="PKM23_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNY89960.1"
                     /translation="MQILITYSSGFGTTREVAEKIGQILQKADLLSVDLLPIDEVSTV
                     APYDTVILGSSIRAGHPLANMVDFIALHRHHLESKRVAIFLVCLCANSEEGRCKAMED
                     QLPQLLRRFPNLHPFAVEAFGGKIDFDKLNPVMRKLMRHVLEQSGLPADGSVDTRDWK
                     LIEQWALRLRDELASAARTSPVTADK"
     gene            complement(10501..11988)
                     /locus_tag="PKM23_00425"
     CDS             complement(10501..11988)
                     /locus_tag="PKM23_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY89961.1"
                     /translation="MSDNSRSPVLASFPRFFWVANLMELFERAAYYGMNSVLAVYLTN
                     SAAQGGLGFSEQSVGFLQSLVYAMTYVVPILGGALADRYGYRRMLMVAFALLTAGYYI
                     TGHVSTYGVIFTSLLVLATGAGLFKPIISGTLARSTTPENSGFGFGIYYWMINLGAFL
                     APLLVSYLKGFSWSLIFTVSALYCALMLLPTLFLYRDPPRPENSKRLREVLGGAAMVL
                     GDARFMLMILVYSCFWILYFQNFGSVLWYLRDFVDKAPVDALFARFGIPFSFDAEHVT
                     VINAGTIILLQVVISRIFKNTGALSTMVIGLFFGALGFLCLAFAATPWIFILGLVIFS
                     IGEMTAHPRYYSYVGTIAPADKTAVYMGYAFLYGVIGSLVGSNLGGLLYARMLKPQIG
                     LASAVEMSRKFWLLFALLGLASMAGLALYNRFFGRDTEAARRRARAIMIGIYSLLLAG
                     GAAFGWSALSGESADYKTLVQSVILVVMGGGGLAVSLRRKPEQAH"
     gene            complement(11998..13263)
                     /gene="larA"
                     /locus_tag="PKM23_00430"
     CDS             complement(11998..13263)
                     /gene="larA"
                     /locus_tag="PKM23_00430"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HNY89962.1"
                     /translation="MELSYGTTILSFLPDPRVAWRILPEVEPVPAAAAGPGILRNAIK
                     QLLGKLKEGGAAAGESLLLVVPDHTRRCRLDEVLGLLLPELEARFAPRVSILVANGSH
                     VVQPDPLVRDLVGDKVYNCYPVAQHDCREEGQLESIGMTSFGTPVIINRAALEADRIV
                     TIGGILYHYFAGFGGGPKMLLPGIAGAETIRINHRRTLNPATGQFHPDCREGNIETNP
                     VYLDLAEIVRFFPAALSLQLVLSPQGEIAAAAAGPILPVQRELVPLVRDFYNLPIGER
                     ADVVIASAGGHPADINLIQSHKSIHHAYQAVKSGGTLIVLAECREGVGSAAFMDYFTG
                     VSAAEMGRELLGNYRINGHTALTLKSKCEGAQIILISALPPELVRRTGMVPAASLAKA
                     WQLAGPNLPAGATGLILPRAAKYLPISQS"
     gene            complement(13285..13746)
                     /gene="bcp"
                     /locus_tag="PKM23_00435"
     CDS             complement(13285..13746)
                     /gene="bcp"
                     /locus_tag="PKM23_00435"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNY89963.1"
                     /translation="MADLHVDDTAPLFSLPATTGKTISLSDYAGKKRVVLYFYPKDDT
                     SGCTKEACSFRDTLPRIDVKDTVVLGVSPDDLKSHQKFIDKYGLNFPLLSDSDHKVAE
                     AYGAWGEKSMYGKKYFGIIRKTFIIGKDGKLEHVFHKVSPEGHGEEILKLI"
     gene            complement(13905..14159)
                     /locus_tag="PKM23_00440"
     CDS             complement(13905..14159)
                     /locus_tag="PKM23_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89964.1"
                     /translation="MNDELIDLKAFWDRDGDGALVYRATAFSESMGREPLFEMTVTFS
                     SSARHHLSDEVCRSIARKLNCEEMRQALEQVESLTDLQHG"
     gene            complement(14164..14643)
                     /locus_tag="PKM23_00445"
     CDS             complement(14164..14643)
                     /locus_tag="PKM23_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89965.1"
                     /translation="MQPLPCRRVLYPYETRVLFLLVGDDQIDKAREIGHLLKESDVAI
                     DRLYTAPETHSLGSAFWRALRPEGQDEEVRIDEVAVGDEESEGLLEEDARRQGSGHCA
                     ELLRLLPKEIRRAPGSHTYAVAPRRNLKIMDGEKDDPHVAEYIIDQRSGQTQLHWLM"
     gene            complement(14896..18036)
                     /locus_tag="PKM23_00450"
     CDS             complement(14896..18036)
                     /locus_tag="PKM23_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNY89966.1"
                     /translation="MKITQLSIKNGVTTAMIYLIVAGFGLFSLARLNVDLYPKLEFPI
                     MAVITQYTGVGPFDLETVVSRPIEEATASVENIKTVRSTSQQGLSLVLLEFNWGTDMN
                     QAEIDVRNSLEFIRDYMPEDASEPMVFAFDVSAQPVLYLTLSSPNLSQAELRYIGEHD
                     LEPRLERIPGVASVSTMGGRKREIKIYADPMRMRAHNIALQQITGALQAANLQIPAGM
                     VENENLEFSVNTVGQFTSVDQIANTAVATYNGSVIRVSDVARVEDGFSEQRQRVWNNG
                     ESSVMVIVQRQSDANTVTVCRAIEKGMPRIKSELPAGVHLDTMMDLSDFITRSMSNLT
                     NTAWQAILMTFLVLLFFLRNIRSAIIVAVSIPVSMVATFAVMDQAGLTLNVISMAGLA
                     LAIGMLVDNSIVVLESIFRRHDEKKDKLVDAANNGTQEVAMAVTASTLTTIVVFLPVL
                     FVPGMAGMLFRDMAITICFSLTISLIVALTLIPLLSSRWLRLKKNLDPNGQLIRVQNR
                     ITGWLDDLHAFYERMLGKALAHKLLVLGTVLLLFVISVGWLFMMGGDFMPQTDDGFVT
                     VTVERSPGTSLEAMEKSMRDLNQIFAEKVEERENIYANFGQGEGMMALFSTRNSSQGE
                     INVKLKERKDRDRSKFEIQDGVREAMTSLPDVDAKFQEGGGMGFSSADITVKIFGHDL
                     AKSEAISNELEPKLKAVEGVVSVTSSMRKQTPELRIDLDRQRIADLGLSTAQIGQVVS
                     TSILGSTVTQFRDGGDEYDVKLQLEKKSRTSKEDLENILIMTPTGAQIPLRALATIDY
                     TRAPREIVREGQERIVTLDLDVSGRDLRGVTADVKKTVNSVAMPQDFRTEIGGSAEDL
                     QESFMYLGIALMVAILLTYMVMASQFESLVDPFTILFTIPLAMIGVALGLILTGTKLS
                     VMALVGVVMLVGIVVNNGIVLVDKINQLRHEGMELEPAIHEAGRVRMRPVLMTAMTTI
                     LGMFPLALGLGESGEIWAPMGRSVMGGMIVSTVLTLIIVPIIYYYMEGIGSRANRFRK
                     EREQRRLDKGIVHAED"
     gene            complement(18033..19160)
                     /locus_tag="PKM23_00455"
     CDS             complement(18033..19160)
                     /locus_tag="PKM23_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNY89967.1"
                     /translation="MQRKMVYGLIILGLSLMGAGCSNKQSNSEQVAAVPVEVTKVQTG
                     RVLQSLTFNGDIEAEFEVKVFSKIPDRIEKYYVDEGSFVQKGAPIARIFATTIQQGVR
                     QAEAGLSAAKAQEANVAVEYERAQRLFKENAMSKQQFDMVVTQYEAAKAGLEQAQAAL
                     LSAKSTLADATVSAPIAGIIGKRYYEAGDMANPAMPVATVVQMERVKVIIDATEGDIG
                     RIRVGQEAEVQVRSFEGETFAGRVSKISPVLDPMTRMAEVEVLVNNNDKRLKPGMFAR
                     VKVITGAVENAIAVPRHAAIEKNTIENVAGEERIVSHYLAYVVVGEKAVQRELEVSYA
                     DHLQLAVTGGLQVGESLIITGQTTLRDGSAVKIITRAEAGK"
     gene            complement(19185..20576)
                     /locus_tag="PKM23_00460"
     CDS             complement(19185..20576)
                     /locus_tag="PKM23_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNY89968.1"
                     /translation="MRLKITHWISIIFLLVVPGLHAQDQLTLTIDESVELALAKNPEI
                     RMAEKELAKARASVWEATANILPQVNGYANLQHAWDIQTQTIPNFLKTMLGPLADMIP
                     ELAGMPDYVQMSFGMENTLVYGASLTQPLFLGGAGMAGIQMATAGKRAAEQNLEAKRQ
                     GLIYQTASAFYACLLAQELAAVQEEALAQAQANLDVVAKKHEVGSASGFDKMRAEVEV
                     ANLKPEVISARNKLQLAITGLRTVLGLPRTSVIRVEGTLAYVEDDFSAMSLAEFQNRA
                     LAARPEILAMKEQKTISGKGVNLAASSFLPKLVFQTDYSYMAMKNNMKFAQGDFSKGF
                     TSAISLQLPLFTGFKNSKQFQKARLDQKIMVDAEKQVFDGIAADAEMSYNKFQEARQK
                     YQAAKESSILAQEALRLANLMYEEGANTQLDVLNSQLAVNRAKLNYVSSLYEYQMARY
                     QLRKAVGQLTAVL"
     gene            20849..22177
                     /locus_tag="PKM23_00465"
     CDS             20849..22177
                     /locus_tag="PKM23_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNY89969.1"
                     /translation="MDLFEEPAVEPNHPGGDAPLADRLRPATLAEFVGQSHLLGQDKI
                     IRRAIESRQLVSMILWGPPGTGKTTLARIIAREGDAHFSALSAVTAGVADVRKIIAQA
                     KQQRRIDGRGTLLFIDEIHRFNKGQQDALLHSVEDGTLLLIGATTENPSFEVIPALLS
                     RCRVYKLEALSTAELGIIIDRALEAENGLKSQRLQLDPEARQLLITLAGGDARNLLTA
                     LELAGRLAHPDAGGVRAIDRELIQEAMQRRTLLYDKQGEYHYDVISAFIKSVRGSDPD
                     AALYWLARMLDAGEDPLFIARRLIILASEDIGNADPHGLMVATAAFQAVHAIGLPEGR
                     IVLAQATTYLACAPKSNASYLALEAAGERVRAGDAVDVPLHLRNAPTRMMKEFGYASG
                     YKYPHDYGGFAEQNYLPEELKTAVFYHPTENGIEKKIKERLLQLWPGRKR"
     gene            22204..24897
                     /locus_tag="PKM23_00470"
     CDS             22204..24897
                     /locus_tag="PKM23_00470"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNY89970.1"
                     /translation="MRTPGLLLLILVLMTAAAPAQAGHSEPLPLAAADTSTTAQPDTL
                     TTTTDSLTTAADSLAAAARAGSRRNSGVDTTVIYQARLIDNDVSARKSYFIGDAQVRY
                     KDMTLKAGKITVDWDAETLTAEGLPDSAWVVNHSGTDSSRQLIIKDRPVLVQSGSEMT
                     GDQMVYNYKTERGRVVRGRTAFEDGRYVGEQIKLVGEKTFNVSNSIYTTCDLDSNPHF
                     HFKARRLKMIVNERVIAKPIVLYLGHIPVAALPFAFFETRTGRHSGVIIPRYGESLQE
                     GRHLRGLGYYWAPNDYFDARATVDYFEKSGWLMELGTNYALRYRLNGAIEGSFTRKNF
                     SSGYTDRRWDLTVRHSQEFSPTSRFSASGYFISDNSYYKDLSINLYTRLTRELRSNAT
                     WSKYWPEQKISLSANFSQVHDLQDDVTQTTLPQISLRKSQTQLFKPGKKTGAGSRRNA
                     RWYHNLYLSYGSNFLNARRESLSMAGGDTTIKVDTDRSLANSLDLSLSSPNKYFGFLA
                     INQSLSIDQDLYDRVHAFSLNPATGTIEDNEENKVAARHTFSYSASANTKIYGVVAPG
                     IGDIQAIRHVVTPSLSFSYRPDFSAKAWGYYTYLQDTSGRTVKRDRFGGTTGSGGSQM
                     VSLSVRNLFQMKRGSGEKVKKIDLFNMDFSTSYNFRAEQYRLSDLRTSWQANPARNFS
                     LSASTGHSFYAWDKSRSTRVNRYLFEDEGWKKGEFLRMTTFNLNFSLRFEGKGEKKQK
                     TETLSDTTGMGADSLRWADDLTVEEENMLRRGRRFQDDRAFGHQSIPWRLNTTFNFFF
                     DKTNPDRPNKRYYLDISGAELSLTKKWRLGYSAHVDLEKGIISYHRCTIYRDLHCWEA
                     SVDWVPSGSGKRVYFRINVKSPSLSDIKLEKFGGAGSVLGY"
     gene            complement(24959..25405)
                     /locus_tag="PKM23_00475"
     CDS             complement(24959..25405)
                     /locus_tag="PKM23_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNY89971.1"
                     /translation="MQNGTDAAPAADNRDYSFPNVNRVAIAGKLIQDPPLRWTTRGVP
                     VTNFVIVTSPEAEADLPEGTDRPPCYISIVVWAQQAVQCNKFLRKGSSVMVIGELQSM
                     PNHAPERNYYPVQVNAQWIQYLEKGTVSPDDAEYGNQGQGELEAGR"
     gene            complement(25429..26196)
                     /locus_tag="PKM23_00480"
     CDS             complement(25429..26196)
                     /locus_tag="PKM23_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89972.1"
                     /translation="MNGRPAPPYHTLFTGGFSPSMVIVQWEESLPPLAGELQTAVDAA
                     WEGLNPNPHFNGRIARLEEWRIRDGALHLWLRPVEYKHLLYSNAHTGEIIRRYGEHQL
                     ARALGISAVVVSAEGQILLMERSDRVGEFPGCYDLFGGHIDPPAQGEIPDPFASMEKE
                     LDEELALLPGTASLSLFGLLLTREARKPELLFIARCGMESEAMMRAALGARDRYEYRR
                     LLAIPADAESVRGFLQRHRTKLSPSAFGGLEIFAAMR"
     gene            complement(26193..26696)
                     /locus_tag="PKM23_00485"
     CDS             complement(26193..26696)
                     /locus_tag="PKM23_00485"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNY89973.1"
                     /translation="MKELFYFIMPWEPSDILVAGPAGAVSRIDFLRGRTPEEALASLS
                     PGWSPRREPAFLPGLQEQLRRYLAGEPVEWKIRIELAGGTDFQRAVWRELQKIPYGRT
                     VSYGEIAARLGKPGAMRAVGGANGANPLPIIIPCHRVVAAAGGLGGYGGGLDIKKALL
                     RLEGASL"
     gene            complement(26845..29559)
                     /locus_tag="PKM23_00490"
     CDS             complement(26845..29559)
                     /locus_tag="PKM23_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY89974.1"
                     /translation="MKLSIRVLLALCLIFVLVSGSYAQTGKITGRVIDSATKQELVGA
                     NVQVVGTSMGAVSDMRGHFTISGVQSGDLKLKVSFVGYETAEKGVMVAAGKTVTANFS
                     LTPTMIMTKGISIFADRAKERETPVAFTNIQKQEMAQRLGSRDIPMVLTTTPSVYATT
                     SGGGAGDARINIRGFDQKNVAIMINGVPINDMENGWVYWSNWDGVSDATSSIQVQRGL
                     SAVNLATPSIGGTMNIITDPSVQKAGITARQEYGTGNFLKSTLSVASGQINNKFSVNG
                     TVVVKQGKGIVDKTYTDAWAYYFGAAYNINANNRLELYALGAPQRHGQNTNKQNIGVF
                     NKEFALGLEGYDPAAAAKYVESSSGVLYNQNWNNVDPSYSGRQFYNGRTHERQTGNVL
                     YERENYYHKPVVNLNWFSKLSDKAGLYSVAYYSGGRGGGSGTYGSVSSDKTRFPNPKD
                     WNATIAKNRASTTGASGVLRNSTNNQDAFGLISKFNYEFSKNLKSAIGVDVRTAEVSH
                     FREIRDMLGGEYYIDLKADKKSPRSDFWSAEQTKRRLGDKIDYDFTNTINWMGLYGQL
                     EATMGKLSAYGTAGWSSVKYDHTNHFKKDAEGNELYLESDWIAGMQLKGGANFRMTDN
                     LDLYANVGHIAKVPIFDNVILDGSSSLAKDPQTEKFLSIEGGINMNLLSNTLAAKLNV
                     YRTAWTDRAVTLATIDAAGDEAIIFLSGLDQLHQGVELELAYQPFKLARLDATVGVGD
                     WGHTSDVSGTYKDYSGDVEKEKSYNLYLDGLKVGDQPQTMLALSASVFPLSGLTMEFT
                     ARHYRDHYSYFNPLGRTSSADRAQSWKAPDYTLLDAHASYTLPVRAGEARFAIYAHLF
                     NLLDEIYIQDAVDNSSYSAFGDKTHAADDAEVWMGFPRNFNIGIQVSY"
     gene            complement(29686..30921)
                     /locus_tag="PKM23_00495"
     CDS             complement(29686..30921)
                     /locus_tag="PKM23_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89975.1"
                     /translation="MKRRYYLLTTLLALFWGCEENKMPLPSPRSASTSFGANDTSYVE
                     LNPVWDSAQLGVPLDAPGDLTISMDGTLFLADEGAGRIHAFSKAGNLLSGSGLDGLVL
                     ESPRALAVDSKLNLLIVNGTNRLFVWNQFLNRVRIDSVADAGLYFDRETGQEITLTFA
                     ELRERLAAGGTQPHLRHYLFRHDPARVAEARGIYPVYADPEPSARFNGVAAGKYGEET
                     VYLTESNFDRIVQLLLVPDLALKGQNRTVLFHYTAELKSNVAVYGSGSGTVDDPWSIT
                     TDDDENLYFTQLGGNFRVQKLLAGSFSPRYVLYQHAIMDLGRFVKPYDVALDDREGIF
                     VLDGESGSVSKFANSGSKAGQLLSLGQKGLALASFNDGRGLLASDDVVYVVERGAKRI
                     RRFQYSVSESDIPDDEKEP"
     gene            complement(30921..31910)
                     /locus_tag="PKM23_00500"
     CDS             complement(30921..31910)
                     /locus_tag="PKM23_00500"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY89976.1"
                     /translation="MTRQRILAFLIFLLAGQAWAQGLLPRLGEQRAGTASMTFLKIGV
                     GARAAAMGGAYVAMANDASATYWNPAGLVQIGRNELVVSHLDWLVDVDFEYLGYVHQL
                     TRTIGLGGFVGYLHFADMPVTTEYHPYGNGDYFSYNDLVAGVSASLKMTDRFSCGITV
                     KYVREQLAELQMGGVLLDLGTYYWTGYKTLRIAAAMRNFGNDIGPEGTYMRRKSGGET
                     ETAWQSFSPPTLFTLGAAMDLFQRSSHLLTGSVQMNHPMDDQENLLLGCEYRYRTLFF
                     LRGGYRANMDENRWTFGAGAKFILKGVHLKIDYAYADYTHLSMSQQFTFGFEF"
     gene            complement(32025..34760)
                     /locus_tag="PKM23_00505"
     CDS             complement(32025..34760)
                     /locus_tag="PKM23_00505"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY89977.1"
                     /translation="MKTTGRIITLAFLLSLCASGALAQSSLIRGRVTDKRTNEALPGV
                     NISVPGTYKGAVTNMEGLYEIRGLAPGDYDLKASIIGYTVQMRTGIHVEPDKTTQADF
                     ALESTVLALGQSVEVIGQKPLFETDITASQQHISSEEIGQKVVESVTDLVGEQLGVVV
                     QDNKIHIRGGRADESLYIIDGQSVKDPLSGYSNTLYVNAAAVKDLKVITGGFDAEYGQ
                     AMSGVVDIETKEGEDHYSGGLSIKSDNWGKGFFPSFNSDIVQINVGGPEPITGKLLPA
                     IGVRIPGKLGFFFSAYGNVSDTYLPRASKLYPSRAAYDKLAPRQENDWHLMGKLSWDL
                     TAGKKLSISHDRSLNINQAYFTSLATDIGGFPYRYSRMLDNYNTLTKEANLTTLQWVH
                     TLSSRTFYRMSLSRFFTSNHSAVRNKHWTEYQEELDLEPIIYIPDSEGNIRTQYGDGF
                     YDTGDAEGWYDYFSDNWTFKGDITTQPNDKHSVKGGFEGRYTDMQVVDINAPWYGESG
                     LGRNHDYFRVYPNDGAFYIQDRITYDGMIVNIGLRYDYWFPGKFVDDAIRDTNTVTIT
                     DAARNKYMDETFELFGLRGKSHLSPRLGISHPVTDNDVLYLHYGHFSQQPKGQYVYAK
                     LRSTSEATYQLFGNPNLKPTTTVAYEIGIKHRFNANQTLEVKAYYKDMFDYPTSTTVR
                     KYSPRYGNISFLMYVNMDYARSRGIEMRFRRRYSKFLSGNIDFTYALATGKSSTPNTN
                     LLVVAGKVSEKPLGESRLSWDRPYRLSTDLYFDMPARANIRLFGLRLPEKWGLTMRWE
                     YESGKRYQRIIDVANNIYQKDEYGSLSKPWMRVDLKWYKEFELFKTRLALLIEGENIF
                     NTKVPRIINPVTGRPYQPGDVIPVTWYDDPDDLPPDNPARFDWPRRVMTGLEVRF"
     gene            complement(34768..36264)
                     /locus_tag="PKM23_00510"
     CDS             complement(34768..36264)
                     /locus_tag="PKM23_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89978.1"
                     /translation="MNYRHGLLLLLAVLFAGCGIKDPFGDDRTSQKLGNLPPQTHLFL
                     HVTQEQITLSDTLLDGTIQTRLYTTGLDTTPSRQIVHWWGDDPDGTVAGYYYQWDYQS
                     EPVFTTGEYDTFYVPIRSQYDEFSFKVWAVDDQGLEDPTPASLRFPVYNTPPSIAFRL
                     NSNPVIAGNPNVVAYTFPTRTFVWDAFDDDGKETITHIYYALDDTSSWIPLAGDADQI
                     TLGDLAPGRHRFFVKAQDISGATSPTLSFPNPDDPNMPLHWEVKPVQGQILLVNDFAQ
                     DQSLYQVQGFYTRILDQLVGPAGYSVWEIGSNTTPLINSQNALPHSSIDIEAYLAYFD
                     KVIWFSHLGRPHLTAAGQGITRFTGKGGKIFISNGNEERPDSTWTFTSLESVYRLNPG
                     GRLLAGVRILASFGTPQDPQLDLSLEKLIGNRVSALVPGAGADVVLRMEPDSTAAVTV
                     PYKGSPPVALRFQIGPGECIYCSLPLHLCNGNANMTEFFRYILFDEFN"
     gene            complement(36346..38967)
                     /locus_tag="PKM23_00515"
     CDS             complement(36346..38967)
                     /locus_tag="PKM23_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY89979.1"
                     /translation="MLLVMLLCAALGTSWAAITPIRDIQFTTEASGDSPLKGQIVTIS
                     GVVTGEIYAFKNNYYYVQDAKAPWSGVKVYDKTHFAAQGDRVTLTGKVEEYKGVTEIT
                     TVTEFTVDSTGTAWLTPMPVTTGEIATGAANAEAYEGCLIRVGASTISNPSLGYGEWQ
                     INDGSGPCRVDDETKYYFRPADYSAVQSVTGILDFANGDTKIEPRLAEDVVEEGPYTR
                     IQRIQQVRYSDLLKTPTKNIMDKSYLLGDTVTVKGIVTMPTGLCYAGAGIKFTFGAAE
                     GGPWSALLSYNADSTAYPTLYEGDEIEMTGYLYEYTTSPSNMTEFFITSPINILDAGK
                     PLPKVDSLKTGDLRWPTTAEQWGTVMVKVGNAVVTNVTPQYELFKVDDGSGGILVDDE
                     SDSMRGYPDPALKSLFKSITGWVYHHYGSYADSTAYKLEPLYRDWLEIGAAGLSIKNT
                     ARSLAAPSPAQGVTITTTLDPMAIAAGAKLWYKVNDGAYASLPMTLNGGVYSAEIPAQ
                     AVNSLVSYYIEATDNTGKLTFDPPDQVAANYSYVVLEGQPTLAQIQYTPWPTADSPFQ
                     DFNVVVEGIVTADTVFFNKYGAYPIQDAAGAWNGLFVLGNVPSLQRGDHVRVYGKVED
                     HNEAYLYKWEGNTQIIADSVRIVAHSQELPAIADVATVDLKDKSASAESYEGVLVRIH
                     NATLVSVNSYDVTVDDGSGPCLLDADAFIGRDQDANPYFYINRTAQLLIVNGDTVRIG
                     DQINVAQGVFLFSFGTHKIEIRNLGDIGTVTGVRNAVTAQPLEFGLEQNYPNPFNPET
                     RLYFQLPASEEVKLIIYNTRGQIVRHLAERRYEAGRHTLNWDGRDNDGNRVPTGVYVY
                     RIKAGDHIAARKMTLVK"
     gene            complement(39022..>39279)
                     /locus_tag="PKM23_00520"
     CDS             complement(39022..>39279)
                     /locus_tag="PKM23_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89980.1"
                     /translation="AEVRIYTLAGDVVDEFIHHGATYKGEDVELMQQRIGGSNTVLPG
                     GLHAWDLISAFDQAIATGLYFFSVKDLQSGEIQTGKFVVIK"
BASE COUNT         8031 a        11794 c        11688 g         7766 t
ORIGIN      
        1 atcggtccat tgataagggc gccctatgcg aattctgatt tacatgtggg agggcatccg
       61 catcgcctat gacgccttgc gcgcctacaa gatgcgttca atactgacca ccctgggcat
      121 tgtcatcggc gtcaccacgg tgatcaccat cgtcgccctg atccagggcc tgaatcaggc
      181 cttttccagc gagatctccg ccctcggcac cgacacggtc tatatccaga aatttccctg
      241 gaccatgcag ggcgacgact ggttcaagta ccgcaaccgg cccaatatca ccgtggacga
      301 ggccgatagg gtgaaagagc tctcccgcac ggtctcggct gtggctccga cagtcgaaac
      361 cagccgcacc atcaagcacc gcgagaaaac ggtcgagcgt gtcagcatca ccggcaccac
      421 cgctgagtac ctgaccaccg ccaatgccga tccagagatc ggccgtttct tcagcgctaa
      481 cgatgtcgcc agcaaccggc ccgtcgcggt gctcggcagt gaagtggccg agctgctctt
      541 cggtcaatcc gatcccctcg gcaagcgcgt caccatcggc ggccgtaagt tccgcgtcat
      601 cggtgtactc ggcaagaagg gctcttttat gggattcaac caggacatcc tggccatcat
      661 cccgatcggc accttccagt ccgccttcgg ggcgcagcgc cgctgggtca ccattgaggt
      721 caaagccaaa tctccgcagc tgattgagga gaccgaagac gaactcaccg gcatcatgcg
      781 ccgtatccgc aaactgactc cggagaagga gagcaatttc gccatcaaca aacaaagtct
      841 gctcctggat acctacaatc agctcaccgg caccctctgg gcggtggcca tcggcgtcgg
      901 ggccatctcc ctgatcgtcg gcggcatcgg catcatgaat atcctcctgg tctccgtgac
      961 ggagcggacg cgtgagatcg gggtacgcaa ggccctgggc gcgcgcaatt cggacatcct
     1021 ctggcagttc ctggtcgaat cgatgatgat ctgctcgatt ggcgtcctca tcggcatcct
     1081 gatggcggtg ggcattgcca aacttgtcgc cgctacaacc cccttgcccg cggccatcac
     1141 ctggtgggtc gcttttctcg gtttggtctt tgtggtcacc atcggcctct ttttcgggat
     1201 ctatccggcc agcaaggcgg cccggctcaa tcccatcgag gccttgcgtt atgaatgact
     1261 ctttatatgc aggctgaacg atggtattaa ccgatacatt tcacctcgcc ctctccgctt
     1321 ttcgcagcaa caagatgcgt tcgctgctga ccctgctggg catcatcatc ggggtcatga
     1381 ccatcatcgc catgcaatcc ctgatcaccg ggctgcgcaa gagcatcaac gagcaggtca
     1441 gctctctcgg cagcagtgtc ttccaggtgc agaaatttcc ggcgatcaac atggggccgc
     1501 atgaccatga taaataccgc aaccgcaagg acatcaccta tgcggacgca cttgcggtac
     1561 gcgaacgggt gactgcagcc aaagctgtcg gcgccgaggt ctggaactgg ggggcggaac
     1621 tccgctacaa agatcgcaag accctgccca acatccaact cgccggagtg acgccggaat
     1681 ttctcgagaa caacgggctc gagatcgctg aaggccgctt tatcaccgcc gaggatgtcg
     1741 acctcaacgt cgcggttacg gtcattggcg cagatgtcgt cgacaaactc tttcctttcg
     1801 aagatccgct cggcaaggac atcaaactca tgggacggag attcgaggta gtcggcatct
     1861 ttgaaaagaa gggcagcgct ttcggccaga gccaggacaa cagaatagcg atcccctttt
     1921 tccagttcac gaggttgtac ggcaaggagc gctcgatcaa catcaccgtc caggccgcca
     1981 cacccgccct ggttgaaacc gccacagagc agaccatcgg tgtgatgagg acgcagcgca
     2041 aggtcaaacc cgggattgaa aatgattttg aaatctattc aaatgaaacc atcaccgggt
     2101 tcttcgacaa cattaccaaa tatgtccgca tcgtcgccat tgccattgcc tcgatctcgc
     2161 tgctggttgc gggagtcggc atcatgaaca tcatgctggt ttcggtaacc gaacgaacgc
     2221 gtgaaatcgg cattcgcaag tcggtcgggg ccaaacgctc cgacatcctt tggcaatttc
     2281 tcatcgaagc gatcgtcctc agtgagttgg gtggcctcat cggcatcatg ctgggacttg
     2341 gcatcggcaa actggtcgat gccctcaccc ccatccccgc cgccgtgccg gtctggacgg
     2401 tctttctcgg attgattttc tgctcttttg tcggtctcgt tttcggggtc tacccggcct
     2461 tgaaagcggc aagaatggat cccatcacag cattgcgtta cgaatagtcc ccgcaaccgg
     2521 gaagaggagg ccgtatggag attgaaaaaa aagtggaatc gggcgtgctg atctttgccc
     2581 tcgagggtga tctgatgggc ggacccgagg cagaggcctt tcagaaagag atactcgcag
     2641 ccattgaagg ggagcaggtt caggtcgtcg ccgatctcag ccgcgtgcgc tggatgaaca
     2701 gctcgggtct gggcatgctc atccgcgccc tgatcagcct gcgcagcagc agcggcgatc
     2761 tccgcctcgc aagcatctcg gagcggctgc gccgcccgat ggaaatcgca cgcctggata
     2821 ccgtttttca gcagtatgat gcggtgacgg aggccattaa tagctttaac tgaaggaggc
     2881 cgtatgatcc agttgagccg ggaagaagtc ttggacaaga tccgcagcgg ccaatcgttg
     2941 gcgggcatca acatgaccga agtcgacctc tcctatgaat ccctcaaggg ggtcgatctc
     3001 tcaggattag acctgcggga agcccaccta caaaactgca acctcgaggg ggccaacctc
     3061 tccggcagca acctcgaaaa cgcctttctc ttcggagcca acctgcacaa ggccaatcta
     3121 agcggggcgc tcctctccga ggccaatctt caggacagta atctcgacca ggccgtcctg
     3181 gtcaaggcga cacttattca cacctcgctc ttcggttcca gccttgaaaa ggccgacctc
     3241 accgatgccg atctgagccg tgcccgcatg aagcgggcca atctgcgcca cgccaccctg
     3301 cgccgcgcca atctccagaa tgtccatatc gagcgggccg acctgaccgg cgccaccctg
     3361 gcgggggccg atctgaccaa cgccaatttt accaacgccg agatcggtca ggccgacttt
     3421 gccgatgcca tccgcgaggg gaccatcgga ctgccctgac caataccacc gcttcgccgc
     3481 actgttatag tgatcgtaga aaagctccag atctctggag cttttacttt tcacttgctt
     3541 ctcaatctat cccatcgtac cttaaaaaaa gttccacaca gggcagacac gtgaatccgc
     3601 aactcgaagc cagattggcc ggatataaaa agctcattca ggggcggctc gaagaggtta
     3661 tccgcaaagg cgagcccgtc tcgctttacg accccatgcg ctatgccctc tcccagccgg
     3721 gcaagcagct ccggccctgt ctgctgctgc tttcctgcga ggccgtcggc ggagacccgg
     3781 agcaggccct ggatggtgcc ctcgccatcg aggtgatcca caacttcacc ctggtccatg
     3841 atgacatcat ggaccatgat gaacttcgcc gcggccggcc aaccgtccac caccgctggg
     3901 acgaaaatgt cgccatcctc gccggagacg ccctcctggt cctgggctat gccctgctgg
     3961 cgcgggtgga tagccgccac ctgcccgcca tcctgcgcac tttcagcagc gccatcctcg
     4021 atatctgtga agggcaggcg ctggacaagg agttcgaaac gcgtgtggaa gtctctttgg
     4081 cgcagtatta tgagatgatc gaaaaaaaaa ccggccggct tttcgagctg gcctgccagg
     4141 cgggcgccat gctcgggggc ggggaagagc agacgagcgc agcgctcgcc tgctacggcg
     4201 ctcaggtggg tcgggcgttt cagctgcagg acgatctgct cgatctgctc ggagaggaaa
     4261 aaaccatcgg caaggatgtg gccagtgatc tggaggaagg taaaaaaagc tttctgattg
     4321 cccacgcgcg gcacaccgcc tctcccgccc agctgatgac cctggcggcg ctgaccgagc
     4381 gcaggcccat cgaaaaagaa cggctcaccg ccatcatcgc cctctttgag gagattggca
     4441 ccctcgcagc ggcccgagca gaaatttccc gctgcctgga aaaagcccgt tcaaatctga
     4501 aagtgctgcc gcaaaatccc gccgcccgcg atctcgaggc ctttatcggt gccatcgccg
     4561 agcggacctt ctgatctgca gcctcaggcc cctgcagtaa aaataaaaag ccccgttgat
     4621 acggggcttt tctgtgtcgg gcgtaattat aataactgct gcggggcgcg cagactggtc
     4681 acgattccac cgcgggccgg cgctgttggg agagcagttg gcagatcggc acgtgcagcg
     4741 gacttttccc cctgcgcctc gccaaacgcc ggcggctctc ctgcagaatg atttctcgaa
     4801 aggagcgcag ccgcaaggcc tcctcgctgt cgctgcaggc gctgcgctgg gagatgcgca
     4861 gatagtcggc ggagatgttc tgcactccac ggtcgagata gtagagggcg cgggttggat
     4921 gtcccgcctc gaggctcatc gcgcacaggg cgaggtaata gatcatgtgc agcgtcggca
     4981 tcatctccat acagctctcg gccgcccccc cgcccttgcc atggcggaag gtgaaacgcg
     5041 ccaactccat gcagtgatgc aggtcctggg cgcaggcctt gtactccccc agcttgagaa
     5101 aagccagggc gcgggggaag tagctgagga gttcctcttc gaggttgcgc tgctcggccg
     5161 tcgagagccc cttgctctct ccctgcgctc tcagcttgcg cgcctcctca cggtagaaat
     5221 ggagataggt tgcacatccc cgggcggaca tgccgtcggg tctgccgctt ctgagcagcc
     5281 tgaaaaagac ggcattttcc agattgatct gaagccgttc acgcccttcc acgacacgga
     5341 tccggcggac ccgcatgctt tgggcgctct gcagccaata atagaggatc ggctcggctt
     5401 cccgattcat gttctcatcc cttcatccct ccacccgatg acggcgaact gctgcgtgcc
     5461 gcccctgagc agtcacatcc ccggtagatc ctgtaaagcg cttgcctggc aacctgctgc
     5521 aaaaattacg cttgtttaat cggcgaaatt gtctaatttt actctatatc acattcttga
     5581 taagtatagc agcagaggcc acaaagtgcg tataaaaaag cacgattttt ccacattccg
     5641 gttttgcagt ggcagtctgg cggcgcagag gaggggccga tttggcggcg catgagcagc
     5701 gggtcgcggt agcaatgagc ggaggggtgg acagttcggt cgctgctctg ctgttgctgg
     5761 aggccaacta cgaggtcctg ggcatcacca tgctccattt tgacgccgac agcgaggaca
     5821 cagccccatc cgcagccgag gaagcggcga tgatctgccg ccacctgggc atcccccatc
     5881 aggtcgtgga tctgcgcgac cgcttccgga aaagcgtcat cgacggtttc gtcgcggaat
     5941 acctggctgg ccgaacaccc aatccctgcg tcctgtgcaa cctcaccatc aagtggggcg
     6001 cactctgcaa attcgccctc gaaagcggag ccgatctttt tgccaccggc cattacgcgc
     6061 gcctcgtgcc cgcccccccc ggcggccgcc atcttctgcg ccgggcggca aacctcgaca
     6121 aggaccaatc ctatgccctc tggcgcctca cccaggagca gctcgcccgg accctctttc
     6181 cgctcggtga tctcaccaaa agcgaggtgc gcgccatcgc caccgcacac ggactgccgg
     6241 ctgcccataa aaccgagagc caggagattt gcttcatcca tgatgacaac tatcagcgat
     6301 ttcttgctga ggagttggaa cagaggggaa tcaccattgc ggagggggag atcatcactg
     6361 tggaaggcaa aagggttgga cgccaccggg gctatcccta ttacaccatc ggacagcgca
     6421 aggggctggg agtggccatg ggccacccgg tcttcgtcac tgccatcgat ccggccgcca
     6481 accgcatcac catcgggcag aaggagcagc tgatggcgac cggactgcag gcgcaaaatc
     6541 ccaactggat cgccgaggcc gagcccagga ggggcgaacc ggtcgaggcg cgcatccgct
     6601 acaaggaccc gggttatccg gccctgatcg aggaggcggg cccggagggc tttaccctcc
     6661 gtttcctcga gccgaggccg gcggtgaccc cggggcagtc ggtcgtcctc tatcgcggcg
     6721 agatcgtcct tggcgggggc atcatcaccc gcgccctctc ctgatccccc tccacctctc
     6781 cgggtgaccg gcatatacag acgccacgcg caccctgctc tccctccccc cttttcccac
     6841 catccttgcg aacgaattaa tgccggtgca gatccttgtg catgagcttg cgggtctcct
     6901 tcaaccggtc atcggccggc agatggagca aatggttgtt gaggtcctcg tcctgctcgg
     6961 cgaagagcag cctcgccgaa tagtcgatga tgctggccgg aaagatgcct tcacgttcgt
     7021 agagagagcg cttttccagc agcagcttcc ccgattcgac gcagctcttc ggcagggcct
     7081 gcagccgctg caggagttgg ggatcctcaa agatattgcc ccgcacataa agctgttccg
     7141 cgagctcgag cgatcctgga tgggtgaggc cccattcagc cgccagggtc attccggcca
     7201 gcaaaagatg gaccgccgca ctgccgtcag gactgcgcag ttcgaccgtc tgccggccag
     7261 cggagctctc cttctccaca tcctgctggg gattgagttt gttcccgaga tcattggcat
     7321 tgacccagcc cagaggcacg cgaatcatcg ccgaacggtt gcaatcgctc cagcagatgc
     7381 gggtcggggc ctcctggttg ggcaccaggc gcagataagc aggtgaaatg gtgttgccaa
     7441 aggcggtgag ggaatcggca taggtgcaaa accccccgat gagccggcgc gcctccgtgg
     7501 tgagccggcc gtcggtgccc agcatgacgt tgcgctcatc cttgcagagt tcgacgtgga
     7561 catgcatacc gctgccggcg acaccctcct caagcttggg ggtgaaggtg gcgacgcagt
     7621 tgttccggta ggcgacgttg cggatgagcc atttcgccag caccaggatg tcgcccgctt
     7681 catcgatggg cgtgggcagc atctcgatct ccagctgctc ggcgcgcttg ccgttgatct
     7741 cggccagatc gctgtgtaca ttttcgagat aaccgacctc gctgtgggca tacttcactg
     7801 cccccgtgat ctgggtgatg tagcggacca tctcatccag caccttgccg gtcttgatga
     7861 agggcgccga ggcgtgatag ccgcgctgct tgccggccag gaaaatatga ctttccggtt
     7921 ccgccagcag atagaattcc agctcgccaa gggcattgag ggtgaatcct gtgctctttt
     7981 tgaaataggc atgggcattg agcaggatat tgtccggcgc aaaggcggca taggtgccgc
     8041 tgctgtcgag ataacggcag atgaaatcga ggctgttggg gtcgaaggga ttcagaaacg
     8101 cagtcttgta aagggggaca acgtaaagat cggaaaccga ggcgtcgacc attcccttga
     8161 agagagagga gccgtccgcc ctttcaccgt cggcgaggat gtggtcggcc tggctgcggt
     8221 tggccagggg gatcttgagc tccttgagtt tgccatcgag agcggtataa tgaagggtga
     8281 tgcgttccat ctgtctttct tcgatgacgc gcaggagatc gcgccgggtg aactcttcgc
     8341 gaggcttgtc caatagcacc gaaatcggat tagccagggc atagctttct ttcacttccg
     8401 cctccaggat gggtccagga acagtaagcc ggtaagcctt ttccggcaag cgcaagcaat
     8461 ttacagctat aggccttcag gcgcaagcag attttttacg ctgccggcgg ccggcggcgc
     8521 aaattttcgg cccggacata ggaaaaaagc tcgatcagcg ccgcacgcgg cagttccgcg
     8581 ctctcaacgg caaaggcgga ggaacgatag agctcccatt cggccagcag ctgggccggc
     8641 agctcgagcc ggtcgtaaag gggtgaagca ggagggaggt aaaaaatcga ggggccgaca
     8701 aggacaccga gatcgagcag atggtctatg gtcccgcgga tctcggcgta gctctgtccc
     8761 ggcagaccga tgatgagata gatggtgaca aatagaccga gccgctgcgc ccgggcgatg
     8821 acctcctcga gctctccctc cccatgcgga cggtgatatc tccggcgcag gccgggatcc
     8881 tgcgtcacca gggagaggtt gagccgtttg aagcctgcgc ggcgcatcag tccgagagtc
     8941 tcctcatcca ggccggggta gcagatcccg ttcatggccg tcagctcgat atcggcgaaa
     9001 agcggctctc tggtcacccc ctgcagaaac tcccggaacc acgccatccg cccggtcagg
     9061 ttatcatcct caaaattgaa gattcgcacc ccgcgccgct cgtgcagaaa gcgcatctcg
     9121 ctcatgacct cctccaccgg gcggtaggag aggctgcgcc cgaacatgcg atcgacactg
     9181 caaaagtcgc agccgttcgg acaaccccgg ctgcagagca gcgaagcata gcggctgcgg
     9241 ccgatacggt agagatcggc agggagcaga tcgtgggccg gcctcagact gcgccagtcc
     9301 acggccccct tcaccagggg cgcgcccgcc cggtcataaa tctcctgcag gcccgtttcc
     9361 gcagggcctg tgcagaccgc atcgatggcc ggggttctcc gccggcattc agcgccgaaa
     9421 acggtagcgt ggtgcccgcc gatgaaaagg aggagatccc tctccgcctt gatgcggccg
     9481 gcgatctcgt ggacgttctt gtaataggcg gtgaactggc tggagagccc gacagcatcg
     9541 ggctgccagt tcctgacttc ctgaagcagg gactcatcct ccctgccgaa gcggtagaag
     9601 cggttgaaaa agtgcgggga gcggccgaga tacggccgca aatgggccag ggcccggggc
     9661 agggcgagag tgcgcttgtg cagcggggtc agggcatcga caagacgaac ctcatggccc
     9721 gcctcccgca tagcagcggc cgtatagagc agcccaagcg gatagaagcg tatcggagtg
     9781 gtataaaagt cttcgattgc aggttgcagc aataaaatgc gcataagccg aaactaaagg
     9841 aattatttct tcgctgttgc ttgtttataa agtaaatgga acagaataca ccagcccctt
     9901 taccctgggg gtcgttcata gccaaccagt aaggttttta tgcagatcct catcacctac
     9961 agcagtggat tcgggaccac gcgggaagtc gccgaaaaaa tcgggcaaat tcttcaaaag
    10021 gccgaccttc tcagtgtgga tctccttccc attgatgagg tttccaccgt tgccccgtac
    10081 gataccgtca ttctcggcag ttccatccgc gccggccacc ccctggccaa catggtcgat
    10141 ttcatcgccc tgcaccgtca ccatctcgaa tccaaacggg tggcgatttt tctcgtctgc
    10201 ctctgcgcca attccgagga agggcgctgc aaggctatgg aagatcagct tccccagctc
    10261 ctgcggcgtt tcccgaatct ccaccccttc gccgtggagg cctttggcgg caagatcgac
    10321 tttgataaac tcaatccggt catgcgcaag ctgatgcgcc atgtccttga gcaatccggt
    10381 ctgcccgcag acggcagcgt cgatacccgg gactggaagc tcatcgaaca atgggcgctc
    10441 cgtcttcgcg acgaattggc ctccgccgcc cgcacctcac cggtcaccgc agacaaatga
    10501 tcagtgagcc tgctccggct tgcggcgcag gctcacggcc agtccgcctc cccccatcac
    10561 gaccaatatg acagactgca ccaaggtctt gtaatccgcg ctctctccgc tcagggctga
    10621 ccagccaaag gccgccccgc cggccagaag caggctgtag atcccgatca tgatggcgcg
    10681 cgcccgcctg cgcgcagcct ccgtatcccg tccgaagaac cggttataaa gggccagacc
    10741 ggccatgctg gccaggccaa gaagggcaaa gaggagccag aatttgcggc tcatctccac
    10801 tgcgctggcc aggcctatct gcggcttgag catgcgggca tagagcagtc cgccaagatt
    10861 ggaacccacc agcgaaccga tcaccccgta gagaaaagca taccccatat agaccgccgt
    10921 cttgtccgcc ggagcgatcg tccccacgta ggagtagtag cggggatgcg cggtcatctc
    10981 accgatggaa aaaatcacca ggccgagaat aaaaatccag ggtgtagcgg cgaaggcgag
    11041 gcagaggaac cccagggcgc cgaagaagag cccgatcacc atggtcgaga gagcgcccgt
    11101 gtttttaaag atgcggctga tcaccacctg cagcaggatg atggtcccgg cattgatcac
    11161 cgtaacatgc tccgcgtcga aagagaaagg gatgccaaaa cgggcgaaaa gagcgtcaac
    11221 cggggccttg tcgacaaaat cgcgcagata ccagagcacc gagccgaaat tctgaaagta
    11281 aagaatccag aagcaggaat agaccagaat catcagcatg aaacgggcat cgcccagcac
    11341 catagccgct ccgcccagca cctcgcgcag acgcttgctg ttttccggcc tcgggggatc
    11401 gcgatagaga aagagggtgg gcagcagcat aagcgcgcag tagagggccg agaccgtaaa
    11461 gatcagggac caggagaatc ccttgagata gctgaccagc agcggggcca aaaaggcgcc
    11521 aagattgatc atccagtaat agatgccgaa tccgaagccg ctgttctccg gggtggtgct
    11581 gcgcgccagg gtacccgaga tgatcggctt gaacaggcca gccccggtag ccagcaccag
    11641 caggctggtg aagataaccc cgtaggtcga aacatgaccg gtgatgtaat agccggcagt
    11701 gagcagggcg aaggcgacca tgagcatgcg ccggtagcca tagcggtcgg caagggcccc
    11761 gccaaggatg ggcaccacgt aggtcatggc atagaccaga ctttgcagaa agcccaccga
    11821 ctgctccgag aaacccaatc ccccttgcgc ggcgctgttg gtgagataga cggccaggac
    11881 tgaattcatg ccgtaatagg cggcgcgttc aaaaagctcc atcagattgg ccacccagaa
    11941 aaagcgcgga aacgaggcca gtaccgggga gcggctattg tcgctcatag tgtcctttca
    12001 ggattgggag atcggcagat atttggcagc cctgggcagg ataagaccgg tcgcacccgc
    12061 gggcagattc ggaccggcca gctgccaggc cttcgccagc gatgcagccg ggaccatgcc
    12121 ggtgcgccgg accagctcag ggggaagggc cgagatcaga atgatctgcg ccccctcaca
    12181 cttgctcttc agggtcaggg cggtgtggcc attgatacga tagttgccga gcagctcccg
    12241 gcccatctcc gcggcgctca caccggtgaa atagtccata aaagcggcgg accctacgcc
    12301 ctcacggcat tcggccagca caatcagggt gccaccgctc ttgacagctt ggtaggcgtg
    12361 atggatggat ttgtgggatt gaataaggtt gatatcggcg ggatggccgc cggcgctggc
    12421 gatgacgaca tccgcccgct ctccgatcgg cagattgtaa aaatctcgca ccagcggtac
    12481 cagttccctc tgcaccggca gaatggggcc ggcggccgca gcagcgatct ccccctgcgg
    12541 ggaaagcaca agctgcaggg agagggcggc cgggaaaaag cgcacaatct cggccagatc
    12601 aagatagacc ggattggtct cgatgttgcc ctcccggcag tcgggatgaa actggccggt
    12661 ggcgggattg agcgtccggc ggtggttgat gcgaatggtc tcggcgccgg cgatgccggg
    12721 cagcagcatc ttgggtccgc ccccaaagcc ggcaaagtag tgatagagaa tccccccgat
    12781 ggtgacaatc cggtccgcct ccagggcggc gcggttgatg atcaccgggg tgccgaagga
    12841 ggtcatcccg atcgactcga gttggccctc ctcccggcag tcgtgctggg cgacgggata
    12901 acagttgtaa accttatctc caaccaggtc gcgcaccagc gggtccggct gcaccacatg
    12961 gctgccattg gcgacgagga tgctcacccg cggggcgaaa cgggcctcca gttcaggcag
    13021 cagcaggcca agaacctcgt caaggcggca gcgccgggta tggtcgggca cgaccagcag
    13081 caggctctct ccggctgcag cgcccccctc cttcagcttc cccaacagct gcttaatagc
    13141 gttccgcaga atgcccggac cggcggctgc cgctgggacc ggctccacct ccggaagtat
    13201 gcgccaggct acgcggggat caggcaggaa ggaaaggatg gtggttccgt aggagagctc
    13261 catatacgcc cttggccaat aaggtcagat gagtttcagt atctcctcgc cgtgtccctc
    13321 gggagagacc ttgtggaaca catgttcgag tttgccgtcc ttgccaataa taaaggtttt
    13381 ccggatgatt ccaaagtact tttttccata catcgacttt tcaccccagg cgccataggc
    13441 ctcggcaacc ttgtgatcgc tgtcggagag aaggggaaaa ttgaggccgt atttgtcgat
    13501 gaatttctgg tgggatttga gatcgtccgg gctgacccca agcactacag tatccttgac
    13561 gtcgatgcgg ggaagggtgt cgcggaaaga gcaggcctcc ttggtgcagc ccgaggtgtc
    13621 atccttgggg taaaaataga gcaccacgcg ctttttgcct gcataatcgc tcagtgagat
    13681 ggttttgccg gtggttgccg gcaggctgaa taggggagcg gtgtcgtcaa cgtgtaaatc
    13741 tgccatggta tcctcctgca atggtttgtt atactgggtt cagtacatct accaacattg
    13801 gaggaggagc ggctattcca cattatggcg ctgccgcggc tgaagagggc ggtaaatcgc
    13861 ccggccgggc ggccgggttc ggaccacggg gaggccgggc cttttcagcc gtgctggagg
    13921 tccgtcaggc tctcgacctg ttcaagagcc tgacgcatct cctcgcagtt gagcttgcgg
    13981 gcgatggaac ggcagacctc atcactcaga tggtgacggg cggaggagga gaaggtgacg
    14041 gtcatctcga agagaggctc cctccccatg ctctcggaaa aggctgtggc gcgatagacc
    14101 agggcgccgt cgccgtccct gtcccagaag gcttttaggt caatcagttc gtcgttcata
    14161 cgatcacatc agccagtgca gctgggtttg gccgctcctt tgatcgatga tgtactcggc
    14221 aacgtgcgga tcatctttct cgccgtccat gattttcaaa ttacgcctgg gcgccacagc
    14281 ataggtgtga ctgccgggag cccggcggat ttccttggga agaagccgca gcagctcggc
    14341 gcagtggccg ctcccctggc ggcgggcatc ctcctccagc aatccttcgc tctcctcatc
    14401 ccccacggcg acctcgtcta tcctcacctc ctcatcctgt ccctccggtc gaagagcccg
    14461 ccagaaagca ctgccaaggc tgtgggtctc cggtgcggtg taaagccggt caatggccac
    14521 gtcgctctcc ttgagcagat ggccgatttc ccgcgccttg tcgatctggt cgtcccccac
    14581 gagcagaaac aggactcggg tttcataggg ataaagaaca cggcggcacg gcaaaggctg
    14641 cataagatcc tccccaggcc tgtaaaagcc tgatattgga actgcattca tgctttgaac
    14701 gcgctgaacg gggatgcaga ggtatcgcgg tgcagggtgg aaaacgcggt ggcggaagaa
    14761 taatgagcgc tgaaatcaaa ctgccttcta taagggtaac atcagccgct ttggcggtta
    14821 ttcccgttcg caaacaagaa agaaaaaggg ccgcttcttg cgaggcggcc cttgctcccg
    14881 gatcctttat cacgcctaat cttcggcgtg gacgatcccc ttgtcaagcc ggcgctgttc
    14941 acgctccttg cggaagcgat tggcccgcga gccgatgccc tccatatagt aatagatgat
    15001 cggcacgatg atcagggtta aaaccgtgga gacgatcata ccccccatga ccgagcgccc
    15061 catcggcgcc cagatctcgc cgctttcacc gaggcccagg gcgaggggga acataccgag
    15121 gatggtggtc atggccgtca tcagcaccgg gcgcatacgc acacgtcccg cctcgtggat
    15181 cgctggctcc agttccatcc cttcatggcg gagctggttg attttgtcca ccagcacaat
    15241 gccgttgttg accactatgc caaccagcat caccacccca accagggcca tcaccgagag
    15301 tttggtcccg gtcaggatca gacccagggc gacgccgatc atggcgagcg ggatggtgaa
    15361 gagaatggtg aagggatcaa ccagggactc gaactgggag gccatcacca tataggtcag
    15421 caggatagcc accatcaggg cgatgccgag gtacatgaaa gactcctgca aatcctccgc
    15481 cgagccgccg atctccgtgc ggaaatcctg cggcatggcc accgagttaa ccgtcttctt
    15541 gacatcggcg gtgacgccgc gcaggtcgcg gccggagaca tccaggtcga gggtgacaat
    15601 acgctcctga ccttcgcgca cgatctcacg cggggcccgg gtgtagtcga tagttgccag
    15661 cgctcgcagg ggaatctgcg ctccggtcgg ggtcatgatg agaatattct ccagatcctc
    15721 cttgctggtg cgcgattttt tctccagctg gagcttgaca tcgtactcgt cgccgccatc
    15781 gcggaactgg gtgacggtgg agccgagaat gctggtgctc accacctgcc cgatctgggc
    15841 ggtgctcaat cccaggtcgg cgatgcgctg gcggtcgagg tcgatgcgca gctcgggggt
    15901 ttgcttgcgc atgctcgagg tgacggagac cacgccctcc accgccttga gcttgggctc
    15961 cagctcattg ctgatcgcct cggacttggc cagatcgtgg ccgaagatct tgacggtgat
    16021 atccgcgctg ctgaagccca tgccgcctcc ctcctggaac ttggcatcaa catcaggcag
    16081 cgaggtcatc gcttcgcgca caccatcctg aatctcaaac ttggagcggt cccgatcctt
    16141 gcgctccttg agtttcacgt tgatctcacc ttgtgaagag ttccgagtgc tgaaaagcgc
    16201 catcatgccc tcgccctggc caaaattggc gtagatgttt tcgcgttctt ccaccttctc
    16261 ggcaaagatc tgattcagat cgcgcatgct cttttccatc gcctcgaggc tggtgccggg
    16321 agagcgttcc accgtaaccg tcacaaagcc atcatctgtc tgaggcatga aatcaccccc
    16381 catcatgaag agccaaccga cggagatgac aaacagcaac aggacagtgc ccaggaccaa
    16441 taatttgtgc gccagagcct tgcccagcat gcgttcgtaa aaagcgtgca gatcgtcgag
    16501 ccagccggtg atccggttct ggacgcgtat gagctggccg ttgggatcca gatttttctt
    16561 caggcgcagc cagcgcgagg agagcagcgg gatcagggtg agcgccacaa tcaaggagat
    16621 cgtcaacgaa aagcagatgg tgatggccat gtcgcggaag agcatccccg ccatgcccgg
    16681 tacaaagagc acgggcagaa agacgacgat cgtggtcagg gtcgaagcgg tgaccgccat
    16741 ggccacctcc tgggtgccat tattggccgc atctaccagc ttgtctttct tttcgtcatg
    16801 gcgcctgaag atgctctcca ggacaacgat cgagttgtcg actagcatgc cgatcgccag
    16861 agccagaccg gccatggaga tgacattcag ggtcaacccc gcctggtcca tgacagcaaa
    16921 ggtggcgacc atggagaccg gaatcgagac ggccacgatg atagcgctgc ggatgttgcg
    16981 taggaagaag agcagcacca gaaaggtcat caggatcgcc tgccaggcgg tgttggtcag
    17041 attggacatc gaacgggtaa taaagtccga cagatccatc atggtatcca gatggacccc
    17101 ggcaggcagc tcggacttga tccggggcat gcccttttcg atcgcccggc agaccgtcac
    17161 ggtgttggca tccgactggc gctggacgat caccatcacc gaagactcgc cgttgttcca
    17221 gacccgctga cgttgctcgc taaagccgtc ctcgacgcgc gcgacatcgc tgacgcggat
    17281 gaccgaacca ttgtaggtgg cgaccgcggt gttggcgatc tggtcgacgc tggtgaactg
    17341 acccaccgta ttgaccgaaa actcgaggtt ctcgttctcg accatacccg cggggatctg
    17401 caggttggcc gcctgcaggg ctccggtaat ctgctgcagg gcgatgttgt gggcacgcat
    17461 gcgcatcgga tccgcgtaga tcttgatctc ccgcttccgt ccacccatgg tggataccga
    17521 tgccaccccg gggatgcgct ccagccgcgg ctcgaggtca tgctcgccga tgtaacgcag
    17581 ttccgcctga ctgaggttag gagaggagag ggtcagatag aggaccggct gagctgaaac
    17641 atcaaaggcg aaaaccattg gttcactggc gtcctccggc atatagtcgc ggatgaactc
    17701 caggctgttg cggacatcga tctccgcctg gttcatgtcc gttccccagt tgaactcgag
    17761 caggaccagc gagaggccct gctgcgaggt ggaccggacg gtcttgatgt tttccacgga
    17821 ggcggtcgcc tcttcgatcg gtcggcttac cacggtctcg agatcgaagg gtccgacccc
    17881 ggtatactgg gtgatcaccg ccatgatcgg aaactcgagt ttggggtaga ggtcgacgtt
    17941 gagccgggcc agggagaaga ggccaaagcc cgcgacgatc agatagatca tcgccgtcgt
    18001 cacgccgttt ttgatcgaga gctgagttat cttcatttgc ccgcctccgc cctggtgatg
    18061 atcttcaccg ccgaaccgtc gcgcagagtg gtctgtccgg ttataatcag agactcaccg
    18121 acctgaaggc cgccagtgac cgccagctgg aggtgatcgg cgtaagagac ctccagttca
    18181 cgctggaccg ccttttcacc cacgacgaca taggcgagat aatgggagac gatgcgctct
    18241 tcgccggcga cgttctcgat ggtgttcttc tcgatggccg catgccgcgg caccgcaatc
    18301 gcattctcca ccgcgccggt gatcaccttc acgcgcgcaa acatgcccgg tttgagccgc
    18361 ttgtcattat tgttcaccag cacctcgact tcggccatgc gcgtcatcgg atccagcacc
    18421 gggctgatct tgctcacccg gccggcaaag gtctcgcctt caaaactgcg cacctggacc
    18481 tccgcctcct gccccaccct gatcctgccg atatcgccct cagtcgcgtc aataatgacc
    18541 ttgacccgct ccatctgcac caccgtggcc acaggcatcg ccggattggc catgtcgcca
    18601 gcctcgtaat agcgcttgcc aataatgccg gcaatcgggg cagacactgt ggcatcggcg
    18661 agggtgctct tggccgagag cagggccgcc tgggcctgct ccagtcccgc cttggccgcc
    18721 tcatattgcg tcaccaccat atcgaactgc tgtttgctca tcgcgttctc tttgaagagg
    18781 cgctgggcgc gttcatattc gacggcaaca ttggcctcct gggcttttgc cgcactcagt
    18841 ccggcctcgg cctggcgtac gccctgctga atcgtggtgg cgaaaatacg ggcgatgggc
    18901 gctcccttct gaacgaaact cccttcatcg acatagtact tttcaatgcg gtccggaatt
    18961 ttcgaaaaga ccttgacctc gaactcagct tcgatatccc cgttgaaggt cagggactgc
    19021 agaactcggc cggtttgtac tttcgtcacc tccaccggca cagcagcgac ttgctcgcta
    19081 ttactttgtt tattgctgca tccagctccc atcagggaga ggccaaggat gatcagacca
    19141 tataccatct ttcgttgcat cgattttctc cttttcacga ttcactagag cacagcggtt
    19201 agctggccca cggccttgcg cagctggtaa cgggccatct gatattcata gagagaactg
    19261 acataattca atttggctcg gttgaccgcc agctgggagt tgaggacatc cagctgggtg
    19321 ttggctccct cctcgtacat cagattggcc agacgcaggg cctcctgggc gaggatggag
    19381 gattccttgg cggcctgata tttctggcgc gcctcctgga acttgttata actcatttcg
    19441 gcgtcggcgg cgatgccgtc aaagacctgc ttttcggcat caaccatgat cttctgatcc
    19501 agccgcgcct tttgaaactg cttgctgttc ttgaaaccgg tgaaaagggg cagctgcaag
    19561 ctgatcgccg aggtgaagcc cttgctgaaa tcgccctggg caaatttcat attattcttc
    19621 atcgccatgt aggaatagtc ggtctgaaag accagcttgg gcaggaagct gctggcggca
    19681 aggtttaccc ccttgccgct gatggtcttc tgctccttca tagcgagaat ctcgggacgg
    19741 gcagcgaggg cgcgattctg gaactccgcc aggctcatgg cgctgaaatc atcctcgaca
    19801 taggccagcg ttccctcgac cctgattaca ctcgtacgcg gcagcccgag cactgtgcgc
    19861 agcccggtga tggcgagctg cagtttattc ctggccgaga tcacctcggg cttgagattg
    19921 gccacctcca cctccgcgcg catcttgtca aagccagagg ccgagccgac ctcatgcttc
    19981 ttggccacga catcaaggtt ggcctgggcc tgggcgaggg cctcctcctg gaccgccgcc
    20041 agctcctgcg ccagcagaca ggcgtaaaaa gcgctggcag tctgatagat cagtccctga
    20101 cgcttggcct caagattctg ctcggccgcg cgcttgcccg ccgtagccat ctgaatgccc
    20161 gccatcccgg ctccgccaag aaagagcggc tgggtgaggc tggcaccata gaccagggtg
    20221 ttctccatgc cgaaagacat ctgaacgtaa tcgggcatcc ccgccagctc ggggatcata
    20281 tccgcgaggg gtccgagcat ggtctttaaa aaattgggga tggtctgggt ctggatatcc
    20341 caggcgtgct gcagattggc gtagccgttg acctgcggca ggatattcgc agtggcctcc
    20401 cagacactcg cccgggcctt ggccagctct ttttccgcca tgcggatctc gggattcttc
    20461 gccagggcca gctcgacgct ctcatcaatg gtgagggtga gctgatcctg ggcatggagg
    20521 cccggaacaa ccagcaggaa aataatactg atccaatgtg ttatctttag tcgcatcgat
    20581 tcctccacag aattgcccgc cggcaggctt gacccattat tctccgagcg gcagccggcg
    20641 atgccatatt aggtagaacg taaagtccgg ataaaagttc gcgggatata caagctataa
    20701 tatactgctt tttggcctaa tttgttacaa aaaaatccgc attccgggcg acagggagga
    20761 cggaacaaga cactgccccg tttttgcagc ttgcaattcc ctcctatttt gactacattt
    20821 aaaccaaagt aacctttcga ggctgaccat ggacctcttc gaagaaccgg ctgttgagcc
    20881 gaaccatccc ggcggggatg cccccctggc cgatcgcctg cgtcctgcga ccctggcgga
    20941 atttgtcggc cagagccatt tgctcggtca ggacaagatc atccgccgcg ctatcgagag
    21001 ccgccagctg gtatcgatga tcctctgggg tccccccggc actggcaaga ccactctcgc
    21061 ccgcatcatc gcccgggaag gcgacgccca tttcagcgct ctcagcgcgg tcaccgcagg
    21121 cgtggccgat gtgcgcaaga tcatcgcgca agccaaacaa cagcgccgca ttgacggccg
    21181 cggcacgctt ctctttatcg acgagatcca ccgcttcaac aagggccagc aggatgccct
    21241 tctccacagc gtcgaggacg gcaccctgct cctcatcggc gccaccaccg agaatcccag
    21301 cttcgaggtc atccccgccc tgctctcacg ctgccgggtc tacaaactcg aagccctctc
    21361 gacggcagag ctcggcatca tcatcgaccg cgccctcgag gcggaaaacg gtctgaaatc
    21421 ccaacgcctg cagctcgatc ccgaggcacg tcagctcctg atcaccctgg ccggaggcga
    21481 cgcccgcaac ctcctcactg ccctcgaact ggccggacgc ctcgcccatc ccgacgccgg
    21541 cggagttcgc gccattgacc gcgaacttat ccaggaggcg atgcagcgcc gcaccctgct
    21601 ttacgacaaa cagggggaat accactacga tgtcatctcc gcctttatca agagcgtacg
    21661 cggctccgat cccgacgccg ccctctactg gctggcgcgc atgctcgacg ccggcgaaga
    21721 tcccctcttc atcgcccgcc ggctgatcat cctcgcctcc gaagatatcg gcaacgccga
    21781 cccccacggc ctcatggtcg ccaccgccgc cttccaggcc gttcacgcca tcggcctgcc
    21841 cgagggacga atcgtcctgg cccaggcaac aacctatctc gcctgcgccc ccaagagtaa
    21901 cgcctcctac ctcgccctcg aagccgccgg cgaacgggtt cgcgcaggcg atgccgtcga
    21961 tgtccccctc catctgcgca acgctccgac ccggatgatg aaggagttcg gctacgccag
    22021 cggctataaa tatccccacg attacggcgg cttcgccgaa caaaactatc tgcccgagga
    22081 actcaagacg gcagtctttt accatcccac cgagaacggg atcgaaaaga aaatcaagga
    22141 gcggctgctc cagctctggc cggggcgcaa acgatgaact cttaccaccc gtatggcggt
    22201 cgcgtgcgca caccaggcct gctcctcctt atcctggtgc ttatgactgc cgccgccccg
    22261 gcccaggctg gccacagtga gcccctcccg ctggccgctg cagacaccag tacaaccgca
    22321 cagcctgaca cgctcacgac cacaacggac tctctcacga ccgccgccga ctccctcgcc
    22381 gcagctgcca gagctggatc gcggcggaac tccggtgtcg acaccactgt catctaccag
    22441 gcccgtctca tcgataacga tgtctcggcc cgcaagagct atttcatcgg cgacgcccag
    22501 gtccgctaca aggatatgac cctcaaggcc ggcaagatca ccgtggactg ggatgccgag
    22561 accctgaccg ccgaggggct gccggacagc gcctgggtgg tcaatcactc cggcaccgac
    22621 tcttcccggc agctgatcat caaggatcgc cccgtcctgg tgcaaagcgg ctccgagatg
    22681 accggtgacc agatggtcta caactacaag accgaacggg gacgcgttgt ccgcggccgc
    22741 accgctttcg aggacggccg ctatgtcggc gaacagatca agctggtcgg cgaaaagacc
    22801 ttcaacgtct ccaactccat ctataccacc tgcgacctgg acagcaatcc ccattttcac
    22861 ttcaaggcgc gacgcctcaa gatgattgtc aatgagcgcg tcatcgccaa acccattgtc
    22921 ctttatctcg gccatatccc ggtcgccgcc ctgcccttcg ccttcttcga gacccgcacc
    22981 ggccgccact ccggggtgat catcccgcgc tacggcgaaa gtctccagga gggccgccac
    23041 ctccgcggcc tcggctatta ttgggcgccc aacgactatt tcgacgccag ggctaccgtc
    23101 gactatttcg aaaaatcggg ctggctgatg gaactgggca ccaattatgc cctccgctac
    23161 cggctcaacg gcgccatcga gggctccttc acccgcaaaa acttttccag cggctatacc
    23221 gaccgccgct gggacctgac cgtccggcac agccaggagt tcagccccac ctcccgcttc
    23281 agcgccagcg gctatttcat cagcgacaac agctattaca aagacctcag cattaatctc
    23341 tatacccgac tgacccgcga gttgcgttcc aatgccacct ggagtaaata ctggccggaa
    23401 cagaaaatca gcctcagcgc caacttttcc caggttcacg acctgcagga cgatgtcacc
    23461 cagaccaccc tgccgcagat ctccctgcgc aaaagccaga cccagctctt caagcccggc
    23521 aaaaaaaccg gcgccggcag ccgccggaac gcccgctggt atcacaatct ctatctctcc
    23581 tacggctcca atttcctcaa cgcccgcaga gagtccctct ccatggcggg cggtgatacc
    23641 accatcaagg tcgatacaga ccgttcgctg gcgaactcgc tcgatctctc cctgagcagc
    23701 ccgaacaagt atttcggctt cctcgccatc aaccagtccc tcagcatcga ccaggacctc
    23761 tatgaccggg tccacgcctt ctccctcaat cccgccacag gaacgatcga ggataatgag
    23821 gagaacaaag tggctgcgcg ccataccttc agctacagcg cctcggccaa caccaagatc
    23881 tatggggtgg ttgcaccggg catcggcgat attcaggcca tccgacacgt tgtcactcct
    23941 tccctctcgt tcagctaccg gcccgatttt tccgccaagg cgtggggcta ttacacctac
    24001 ctccaggaca cctccggcag aaccgtaaaa agagaccgct tcgggggaac caccggcagc
    24061 ggcggcagcc agatggtgag cctgagcgtg cgcaacctct tccagatgaa gcggggcagc
    24121 ggcgagaagg tcaagaaaat cgaccttttc aatatggatt tcagcaccag ctacaatttc
    24181 agggccgagc agtaccgcct ctccgacctg cgcacctcct ggcaggccaa tcccgcccgc
    24241 aatttcagcc tgagcgccag caccggccac tctttctatg cctgggacaa gagcagatcc
    24301 acccgcgtca atcgctatct cttcgaggac gagggctgga aaaagggcga gtttctgcgc
    24361 atgaccacct ttaatctcaa tttttcgctt cgcttcgaag gcaaaggcga gaagaagcaa
    24421 aagacggaga ccctctccga cacgaccggg atgggcgcgg acagcctgcg ctgggccgat
    24481 gatctgaccg tcgaggagga gaacatgctg cgccgcggcc gccgcttcca ggacgatcgc
    24541 gctttcggcc accagagcat cccctggcgg ctcaacacca ccttcaattt tttcttcgac
    24601 aaaaccaatc cggaccggcc gaacaaacgc tactacctcg atatctccgg cgccgagttg
    24661 agcctgacca aaaagtggcg gctcggctac agcgcccatg tcgatctcga aaagggaatc
    24721 atctcctatc accgctgcac catctaccgc gacctgcact gctgggaggc ctcggtcgat
    24781 tgggtaccca gcggcagcgg caagcgcgtc tacttccgca tcaatgtcaa gtcgccgagc
    24841 ctcagcgaca tcaagctgga gaaatttggc ggcgccggca gcgtgctcgg atactgatcc
    24901 aaaaaaaaag cgcgcccagc aggggcgcgc ccgatcattt tcctgttcca gctccccttt
    24961 accgcccggc ctccaactcc ccctgcccct gattgccata ctccgcatcg tccggggaga
    25021 ccgtcccctt ttccagatac tggatccact gggcattgac ctgaacagga tagtaattgc
    25081 gctccggggc atgattcggc atcgactgca gctcgccgat gaccatgacc gagctgccct
    25141 tgcgcaggaa cttgttgcac tgcaccgcct gctgcgccca taccacaatg ctgatatagc
    25201 agggggggcg atccgtaccc tcgggcaaat cggcttcagc ctccggcgag gtgacaatga
    25261 cgaaattggt gaccggcaca ccccgcgtcg tccagcgcag gggcggatcc tggatcagct
    25321 tgccggcaat cgccacccgg ttgacgttgg ggaaggagta gtctctgttg tccgcggcgg
    25381 gggcggcgtc tgtgccattc tgcataaaac aatacctcgt gaaagtctct atctcattgc
    25441 tgcaaatatt tcaagccccc cgaaggctga aggactgagc ttcgtccggt ggcgctgcag
    25501 aaatcctctg acggattcgg catcggccgg gatggccagt agccgcctgt actcataccg
    25561 gtcccgcgcg ccaagagccg cgcgcatcat cgcctcgctc tccatgccgc agcgggcgat
    25621 gaagagcagc tcgggtttgc gtgcctccct tgtcagcagc aagccgaaga ggctcagcga
    25681 cgccgtgccg gggaggagag ccagctcctc atccagctcc ttctccatgc tcgcaaaggg
    25741 atcggggatc tccccctggg cggggggatc gatatggccg ccgaaaagat catagcagcc
    25801 gggaaactcg cccacccggt cactgcgttc cataagcaaa atctgcccct ctgcgcttac
    25861 caccacggcg ctgatgccga gggctcgcgc cagctggtgt tcgccatagc ggcggatgat
    25921 ctcacccgta tgggcgttgg agtagagcag atgtttgtac tcaaccggac gcagccacag
    25981 gtgcagcgcg ccatcccgga tcctccactc ctccagccgg gcgatacggc cgttgaagtg
    26041 gggattgggg ttcagccctt cccaggccgc gtcgacggcg gtctgcaact ccccagccag
    26101 gggcggaagg ctctcctccc attgcacaat caccatggaa ggggagaagc ctccggtgaa
    26161 gagggtgtgg tagggcggcg ccggcctccc gttcaaaggc ttgcgccctc gagccgcagc
    26221 agggcttttt taatatcgag cccgccaccg taacccccga gccctccggc agcggccacc
    26281 acacggtggc aggggatgat gatcggcagc gggttggcgc cgttggcccc acccaccgcc
    26341 cgcatggcgc cgggcttgcc cagccgtgcg gcgatctccc cgtagcttac ggtccggccg
    26401 taggggatct tttgcagctc ccgccagacc gcgcgctgaa agtccgtgcc ccccgccagc
    26461 tcgatccgga tcttccattc caccggttca ccggctagat agcggcgcag ctgctcctgc
    26521 agcccgggca ggaaggcggg ctcccggcgt ggggaccagc cgggcgagag ggaggccagc
    26581 gcctcctccg gagtacggcc gcgcagaaaa tcgattcggc tcaccgcgcc ggccggaccc
    26641 gccaccagga tatcgctggg ttcccagggc atgataaaat aaaagagttc tttcattctg
    26701 aaaaagtatc catatttacg cttttagtca agcgaaaagt tgccccctcc cggcgggcac
    26761 ggcagcctga cggttttggc tccggcgccg cgcccccgga aacacaaaag cccccatgcg
    26821 ggggcttttg tgcgaagaaa tgtctcagta ggagacctga attccaatgt tgaaattgcg
    26881 cggaaagccc atccagacct cggcgtcatc agcggcgtgg gtcttgtcgc caaaggcgct
    26941 gtagctgctg ttatccactg catcctggat gtagatctcg tccagcaggt tgaagaggtg
    27001 ggcatagatc gcaaacctgg cttcacccgc gcgcaccggc aatgtgtagg aggcatgggc
    27061 atcgagcagg gtataatccg gcgctttcca gctctgggcg cgatcagccg aactggtgcg
    27121 accgagcgga ttgaaatagg agtaatgatc gcgataatga cgggcggtga actccatggt
    27181 caacccggag agagggaaaa cactggcgct gagggccagc atggtctggg gctggtcgcc
    27241 gaccttgagg ccgtcgagat agaggttgta ggatttttcc ttctcgacat cgccggagta
    27301 atccttgtag gtgccgctga cgtcgctggt gtggccccag tcgccgacgc caacagttgc
    27361 atccagacgg gccagcttga agggctgata ggccagttcc agttcgacgc cctggtgaag
    27421 ctgatccaga cccgacagga agatgatggc ctcatcaccg gcagcatcga tcgtcgccag
    27481 ggtgacggcg cgatccgtcc aggcggtgcg atagacgttc aacttggcgg cgagggtgtt
    27541 gctcagcaga ttcatattga tgccgccctc gatcgagagg aacttttcag tctggggatc
    27601 cttggccaga gaggaagagc cgtccagaat aacattgtca aagatgggca ccttggcgat
    27661 atggccgaca ttggcataaa gatcgaggtt gtcggtcatg cggaagttgg cgccgccctt
    27721 gagctgcatg ccggcgatcc agtcgctctc cagatagagc tcgttgcctt cggcatcctt
    27781 cttgaaatgg ttggtatggt catactttac gctcgaccat ccggccgtac cgtaggcgct
    27841 gagcttgccc atggtcgcct cgagttgacc gtagaggccc atccagttga tcgtgttggt
    27901 aaagtcgtaa tcgatcttgt cgcccagacg gcgtttggtc tgctcggcgc tccagaaatc
    27961 gctgcgcggg ctctttttgt ccgccttcag gtcgatatag tactcaccgc ccaacatgtc
    28021 gcggatttcg cggaagtggc tgacttcggc ggtgcgcaca tccacgccga tcgcactctt
    28081 gagattcttg ctgaattcat agttgaactt ggagatgagg ccgaaagcgt cctggttgtt
    28141 ggtgctgttg cggagcacac ccgaggcgcc ggtggtgctg gcccggttct tggcgatagt
    28201 ggcgttccag tccttgggat tggggaaccg ggtcttgtcg ctggagaccg agccgtaggt
    28261 acccgaacca ccgccgcggc cgcccgaata ataggccacc gagtagagac cggccttgtc
    28321 gctgagcttg gaaaaccagt tcagattgac caccggtttg tggtaatagt tttcccgctc
    28381 atagagcaca ttgccggttt gacgctcatg cgtcctgccg ttgtagaact ggcgtcccga
    28441 ataggaggga tccacattgt tccagttctg attgtagagc acgccggagc tcgattcgac
    28501 atactttgcg gccgccgccg gatcatagcc ttcaaggccg agggcaaatt ccttgttgaa
    28561 gacaccgata ttctgcttgt tggtgttctg gccgtggcgc tggggggcgc ccagggcata
    28621 gagctccaga cggttgttgg cgttgatatt ataggcggcg ccgaaataat aggcccaggc
    28681 atcggtatag gtcttgtcga cgataccctt accctgcttg accaccactg taccgttgac
    28741 cgaaaattta ttgttgatct ggccgctggc gacgctgagc gtcgacttga ggaaattgcc
    28801 ggtgccgtac tcctgacgtg ccgtgatgcc ggccttctgc acggaaggat cggtgatgat
    28861 gttcatcgtg ccgccgatgg aaggggtggc cagattgacg gcgctcagac cgcgctgaac
    28921 ctggatcgag gaggtggcat cggagacacc atcccagttg gaccagtaaa cccagccgtt
    28981 ctccatgtcg ttgatcggca caccgttgat catgatggcc acattcttct gatcgaagcc
    29041 gcggatgttg atgcgggcat cacccgcgcc gccgcccgag gtcgtggcat agacgctcgg
    29101 ggtcgtggtc aggaccatcg gaatatcacg ggagcccagc cgctgggcca tctcctgctt
    29161 ctgaatattg gtaaaggcga ccggcgtctc acgctctttg gcgcggtcgg cgaaaatgct
    29221 gatgcccttg gtcatgatca tggtcggggt caggctgaaa ttggccgtta ctgtcttgcc
    29281 ggctgcaacc ataacaccct tttcggccgt ttcatagccc acaaacgaga ccttgagctt
    29341 caggtcaccg gactgaacgc ccgaaatcgt aaagtggccg cgcatatcgg aaaccgcgcc
    29401 catgctggta ccgaccacct ggacgttggc cccaaccaac tcctgcttgg tggcagagtc
    29461 gattacgcga ccggtgatct tgccggtctg agcgtaagac ccgctcacga gcacaaaaat
    29521 caaacagaga gcgagcaaaa cccgaattga tagcttcata aatccactcc tcctttggac
    29581 tggctgtagc tggaattatc cgaataaata agccgcactt cctccactcg catactggcg
    29641 atctcagaga tagccgctgc acctcctttc atgtacaata ctggatcatg gctccttttc
    29701 gtcgtccggg atatcggact ccgaaaccga gtattgaaag cgacgaatgc gtttggcgcc
    29761 gcgctcaacc acatagacca catcatcact ggccagcagc cccctgccgt cattaaagct
    29821 ggccagcgcc agcccctttt gcccgagaga aagcagctgc ccggccttgg aaccggaatt
    29881 ggcaaatttg ctcactgaac cgctctcgcc atccaggaca aagatgccct cccgatcgtc
    29941 cagggccaca tcgtaaggct ttacaaagcg accaagatcc atgatggcgt gctgataaag
    30001 aacataacgc ggactaaagc tcccggccag cagcttctgc acccggaagt tgccgccaag
    30061 ctgggtgaag tagaggttct cgtcatcatc ggtggtgatg gaccaggggt cgtcgactgt
    30121 acccgatcct gaaccataga ccgccacatt gcttttcaac tctgctgtat agtggaaaag
    30181 tacagtccga ttctgtccct tgagcgccag atccgggacc agaagcagct gtacgatccg
    30241 gtcaaagttg gattcagtca gatagacggt ctcctcgccg tatttgcccg ccgcgacgcc
    30301 gttgaagcgg gcggagggtt ccggatccgc atagaccgga tagatacccc gggcctccgc
    30361 cactcgcgcg gggtcatggc ggaaaaggta gtggcgcagg tggggctgcg tccctccagc
    30421 ggccaaccgc tccctaagct ccgcgaaggt gagggtgatc tcctggcctg tctcgcggtc
    30481 gaaatagagt ccggcatcag cgaccgaatc gatgcgcact cggttcagaa attgattcca
    30541 tacgaagagg cggttcgtac cgttgacaat gagaagattg agcttgctgt ccacggccag
    30601 ggcgcgcgga gactccagga caaggccatc caggccgctg ccgctgagaa gattgcccgc
    30661 cttgctgaag gcgtggatcc ggcccgcccc ctcatcggcg agaaagaggg tgccatccat
    30721 gctgatagtc aggtcccccg gggcatccag ggggacaccc agctgggcgc tgtcccacac
    30781 cggattgagc tcaacgtagc tggtatcgtt ggcgccgaac gaggtgctgg cgctcctcgg
    30841 gctgggcagg ggcatcttgt tctcttcaca accccagaag agggcgagaa gggtggtcag
    30901 gagataatat cgtctcttca tcaaaattca aacccgaatg tgaactgctg cgacatggat
    30961 agatgggtat aatcggcgta ggcgtaatcg attttgaggt gaaccccctt gaggatgaac
    31021 ttggcccccg ccccgaaggt ccaccggttt tcatccatat tggcgcgata gccgccgcgc
    31081 aggaagaaga gggtccggta acggtattcg cagccgagaa ggaggttctc ctgatcatcc
    31141 atgggatggt tcatctgcac gctgccggtg agaagatggc tgctgcgctg gaacagatcc
    31201 atggcggcgc cgagggtaaa cagggtcggc ggggaaaagg actgccaggc ggtctcggtc
    31261 tctccgcccg acttgcggcg catgtaggtg ccttccgggc cgatgtcatt gccaaagttg
    31321 cgcatcgccg cagcgatgcg cagggtcttg taaccagtcc agtagtaggt gccgaggtcg
    31381 agaagaacgc cccccatttg cagctcggcg agctgctcgc gcacatactt gaccgtgatc
    31441 ccgcaggaga agcggtcggt cattttaagg gaggcactca cgccggcgac cagatcattg
    31501 taggaaaagt aatcgccatt gccgtaggga tggtattcag ttgtcaccgg catgtccgcg
    31561 aaatggagat agccgacaaa gccgccgagc ccgatggtgc gggtgagctg gtgaacatag
    31621 cccaggtact cgaaatcgac atcgaccagc cagtcgagat gggagaccac cagctcgttg
    31681 cggccgatct gcaccaggcc ggccgggttc cagtaggtgg cggaggcatc attggccatg
    31741 gcgacatagg cgccgcccat ggcggccgcg cgggccccca cgccgatctt gagaaaggtc
    31801 atcgaggccg ttccggcgcg ctgctcgcca agcctgggca gcagcccctg cgcccacgcc
    31861 tgtccggcga gcaggaagat cagaaaagct agtatacgct gtcttgtcac gtaatccgtt
    31921 ctcggtcgaa aatgtaacgt aactgaggaa gactttctca tcggagcgca gaaaaatcag
    31981 gccgtatcat gcgctgagca agccgtaccc cgcgtgaaaa ggatttagaa cctcacctcc
    32041 agacccgtca tcacccgccg cggccagtca aagcgcgcgg gattgtccgg cggcagatca
    32101 tcgggatcgt cataccaggt gaccggaatg acatctcccg gctgataggg acggccggtg
    32161 acaggattga taatgcgcgg caccttggta ttgaagatgt tttccccctc gatcagcagg
    32221 gcgagtctgg ttttgaagag ttcgaactcc ttgtaccatt tgaggtccac gcgcatccag
    32281 ggtttggaga gcgagccgta ctcgtccttt tgatagatat tgttcgccac atcgatgatg
    32341 cgttggtaac gcttgcccga ctcatattcc cagcgcatgg tcaggcccca tttttccggc
    32401 aggcgtaagc cgaagaggcg gatattggcc ctggccggca tgtcgaagta gagatcagtg
    32461 gagagtcggt aggggcggtc ccagctcagc cggctctcgc ccagcggctt ttccgaaacc
    32521 ttgccggcga cgacgagcag attggtgttc ggagtagaac ttttgcccgt ggccagggcg
    32581 taggtgaaat cgatattacc gctcagaaat ttgctatagc gcctgcgaaa gcgcatctcg
    32641 atgccgcgcg aacgggcgta atccatgttg acgtacatga ggaagctgat gttgccgtaa
    32701 cgcggactgt atttccgcac cgtggtcgag gtgggatagt cgaacatatc cttgtaatag
    32761 gccttgactt ccagggtctg gttggcattg aagcggtgct tgatgccgat ctcgtaggct
    32821 acggttgtcg tcggctttag gttgggattg ccgaagagct gataggtcgc ctcggaggtc
    32881 gagcgcagct tggcatagac atactgcccc ttgggctgct gggagaaatg gccatagtgg
    32941 agatagagca catcgttgtc ggtgaccggg tggctgatgc ccagacgggg ggagagatgg
    33001 ctcttgccgc gcaggccgaa gagctcgaag gtctcatcca tatatttatt gcgcgcggcg
    33061 tcagtgatgg tcacggtgtt ggtgtcgcgg atcgcgtcat cgacaaattt gccggggaac
    33121 cagtagtcat agcgcaggcc gatgttgacg atcatgccgt cgtaggtgat gcggtcctgg
    33181 atgtaaaagg ccccatcgtt gggatagacc ctgaaatagt cgtgattgcg ccccagtccg
    33241 ctctcgccgt accagggggc attgatgtcg accacctgca tgtcggtata gcgtccctcg
    33301 aagccgccct tgacggagtg cttgtcgttc ggctgggtgg tgatatcgcc cttgaaagtc
    33361 cagttgtcgc tgaaataatc gtaccacccc tccgcatcgc cagtatcata aaagccgtcg
    33421 ccgtactggg tgcggatgtt cccctcagaa tcaggaatgt agataatggg ttcaaggtcg
    33481 agctcttcct gatactcggt ccagtgcttg ttgcgaacgg cggaatggtt gctggtgaaa
    33541 aagcgcgaga ggctcatgcg gtaaaaggtc ctgctgctga gggtatggac ccactgcagg
    33601 gtggtcaggt tggcctcttt ggtcagggta ttatagttgt cgagcatccg ggagtagcgg
    33661 taggggaagc cgccgatatc ggtcgccagc gaggtgaaat aggcctgatt gatgttgagc
    33721 gagcggtcat gggagatgga gagttttttg ccggcggtga gatcccagga aagcttgccc
    33781 atgaggtgcc agtcgttctc ctggcgcggg gccagcttgt cataggcggc cctggagggg
    33841 taaagcttgc tggcgcgcgg caggtaggta tcgctgacat tgccgtaggc gctgaagaag
    33901 aaaccgagct tgccgggaat gcgtacgcca atcgcaggca ggagcttgcc ggtgatgggt
    33961 tcggggccgc cgacgttgat ctggacgatg tcggagttga aggaggggaa gaagcccttg
    34021 ccccagttgt ccgatttgat ggagagtccg cccgagtagt gatcctcgcc ctccttggtc
    34081 tcgatgtcga cgaccccgga catggcctga ccgtactcgg cgtcgaatcc gccggtgatg
    34141 accttgagat ccttgaccgc cgcggcgttg acatagaggg tgttggaata gcccgagagg
    34201 ggatccttga ccgactggcc gtcgatgatg tagaggctct cgtcggcgcg cccgccgcgg
    34261 atgtgaatct tgttgtcctg gaccaccacg ccgagctgct cgccgaccag gtccgttacg
    34321 ctctcgacta ccttctggcc gatctcctcc gaggagatat gctgctggga ggcggtgata
    34381 tcggtctcaa agaggggttt ctggccaatg acctcgacgg attggcccag ggccagcaca
    34441 gtggactcga gcgcaaaatc ggcctgggtg gtcttatccg gctcaacatg gatgccggtg
    34501 cgcatctgga cggtatagcc gatgatggag gccttgagat cgtaatcccc gggagcgagg
    34561 ccgcggattt cgtagagccc ctccatattg gtcaccgccc ccttgtaggt gccgggcacg
    34621 gagatattga cacccggcaa ggcttcgttg gtgcgcttgt ccgtcacgcg cccccggatg
    34681 agggaggatt gagccagtgc gccggaggcg caaaggctca acaagaaagc gagggtgatt
    34741 atcctgccgg ttgtcttcat gggtctgtca gttgaactca tcaaagagga tatacctgaa
    34801 aaattctgtc atgttggcgt tgccgttgca aagatggagt ggcagagagc agtagatgca
    34861 ttctcccggt ccgatctgga aacggagggc gacgggcggc gatcccttgt agggcacggt
    34921 gaccgcggcc gtgctgtcgg gttccatgcg caggacgaca tcggcgccgg cccctggaac
    34981 cagggcggag acgcggttgc cgatcagctt ctcgaggctg agatcgagct gggggtcctg
    35041 gggggtgccg aagctggcga ggatcctcac accggccagg agccggccgc ccggattgag
    35101 gcggtagacc gattccagac tggtaaaggt ccaggtgctg tcgggcctct cctcattacc
    35161 gttgctgata aagatcttgc cgcctttgcc ggtgaagcgg gtgatgccct ggccggcggc
    35221 ggtgagatgg ggacggccga gatggctgaa ccagatcacc ttgtcgaaat aggccaggta
    35281 agcttcgata tcgatcgagg aatggggcag ggcattctgc gagttgatca ggggcgtcgt
    35341 gttggagcca atttcccaga ccgaataacc cgccgggccg accagctgat cgagaatccg
    35401 ggtgtaaaaa ccctgcacct ggtagaggct ctggtcctgg gcgaaatcat tgaccagcag
    35461 gatctgcccc tgcaccggct tgacctccca gtgcagcggc atgttgggat cgtccggatt
    35521 gggaaaggag agagtcgggc tggtcgcccc ggaaatgtcc tgggccttga cgaaaaagcg
    35581 gtgccggccg ggggccagat ccccgagggt gatctggtcg gcatcccccg ccagtggaat
    35641 ccagctgctg gtgtcatcca gggcgtaata aatatgggtg atggtctcct tgccgtcatc
    35701 gtcgaaagcg tcccagacaa aagtacgcgt cgggaaggta taagcgacaa cgttggggtt
    35761 gccggcgatg accggattgg agttgagccg gaaggcgatg gagggggggg tgttgtaaac
    35821 cgggaagcgc agcgaggccg gggttggatc ctccagcccc tgatcatcca ccgcccaaac
    35881 cttgaagctg aactcgtcat actggctgcg gatgggcaca taaaaggtgt cgtactcgcc
    35941 agtggtgaag accggctcac tctgatagtc ccactgatag taataaccgg ccacggtgcc
    36001 atccggatca tccccccacc agtggacgat ctggcggctg ggcgtggtat ccagcccggt
    36061 ggtgtataac cgggtctgga tggtgccgtc gaggagggta tcgctcaggg tgatctgctc
    36121 ctgggtcaca tggagaaata gatgggtctg tggaggcaaa ttgcccagct tctgcgaggt
    36181 gcggtcatcc ccgaagggat ccttgatccc gcacccggcg aagagcactg ccaggagaag
    36241 cagaaggccg tgtctgtaat tcatgttcat ccggataaat ctgcacgtct acaaaaagca
    36301 gtcaggtcca tcggctcttg tgccggtgga cctgatgaat tgactttatt taaccagggt
    36361 catcttgcgc gcggcgatat gatcgccggc cttgatgcga tagacataga cgcccgtcgg
    36421 cacccggttg ccgtcgttgt cgcggccgtc ccagttaagg gtgtggcggc ccgcttcata
    36481 ccggcgctcg gccagatggc ggacgatctg gccgcgggtg ttgtagatga tcagtttgac
    36541 ctcttcggag gcaggcagct ggaagtagag cctcgtctcc ggattgaagg gattgggata
    36601 gttctgctcc aggccgaact cgaggggctg cgctgtcacc gcattccgga cgccggtcac
    36661 cgtgccgatg tcgcccagat tgcggatctc gatcttgtgg gtgccgaagc tgaagaggaa
    36721 gacaccctgg gcgacgttaa tttggtcgcc gatccgaacc gtatccccgt tgacgatcag
    36781 cagctgggcg gtgcggttga tgtagaaata ggggttcgca tcctgatcgc ggccgataaa
    36841 agcgtcggca tcgagcaggc acgggccgct gccgtcatcg acggtgacgt cgtaagagtt
    36901 gaccgaaacc agggtggcat tgtggatgcg caccagcacg ccctcgtagg actcagccga
    36961 ggcgctcttg tccttgagat cgacggttgc cacatcggcg atcgcaggca gttcctggct
    37021 gtgggccacg atccggacgc tgtcggcgat gatctgggtg ttgccctccc atttgtagag
    37081 ataggcctcg ttgtggtcct ccaccttgcc atagacgcgg acatgatccc cgcgctgcag
    37141 actgggaaca ttgcccagga cgaagaggcc gttccaggcg ccggcggcgt cctggatggg
    37201 gtaagcgcca tatttgttga agaacacggt gtcggcagtt acgatgcctt ccaccaccac
    37261 attgaaatcc tggaaggggc tgtcggcggt gggccagggg gtgtactgaa tctgggccaa
    37321 tgtcggctgg ccttccagca cgacatagga gtaattggcg gcgacctgat cggggggatc
    37381 aaaggtcagc ttgccggtat tgtcggtggc ctcgatgtaa tagctgacca ggctgttgac
    37441 cgcctgggcg gggatctcgg cgctgtagac gcctccattg agggtcatcg gcaggctggc
    37501 ataggccccg tcattgacct tgtaccagag cttcgccccg gcggcgatgg ccatgggatc
    37561 cagggtggtc gtgatcgtca caccctgcgc gggcgagggg gcggccaggc tgcgcgcggt
    37621 gttcttgatg ctcaggccgg cggcgccgat ctccagccag tctcggtaga ggggctccag
    37681 cttgtaggcg gtggaatcgg cataggagcc gtaatggtga tagacccagc cggtgatcga
    37741 tttgaacaag gacttgaggg ctggatccgg atagccgcgc atgctgtccg attcgtcatc
    37801 gacgaggatg ccgccgctgc cgtcatcgac cttaaagagt tcatactggg gagtgacatt
    37861 ggtgaccacc gcattgccca ccttcaccat cacggtcccc cactgctcgg ccgtggtcgg
    37921 ccagcgcaga tcgccggtct tgagggaatc gaccttgggc aggggcttgc cggcatcgag
    37981 gatgttgatc gggctggtga tgaaaaactc ggtcatattg gagggagagg tggtgtactc
    38041 gtagaggtac ccggtcatct cgatctcatc gccctcatag agggtgggat aggcggtaga
    38101 gtcggcgttg taggagagca gcgccgacca gggcccgccc tcagcggcgc cgaaggtgaa
    38161 tttgatcccg gcgccggcat aacagaggcc ggttggcatg gtgacgatgc ccttgacggt
    38221 cacggtatcg ccgagcaaat agcttttgtc cataatgttc ttggtgggcg tcttgagcag
    38281 atcgctgtag cgcacctgct ggatgcgctg gatgcgggtg tagggaccct cctcaaccac
    38341 atcctcagcc aggcgcggct cgatcttggt atcgccgttg gcaaaatcga ggatgccggt
    38401 gaccgactgg accgcggaat aatcggccgg gcggaaatag tacttggttt cgtcgtcgac
    38461 gcggcagggg ccggagccgt cattgatctg ccactcgccg tagccgaggc tcgggttgga
    38521 gatggtggag gcgccgaccc ggatgaggca gccttcgtag gcctcggcgt tggcggcgcc
    38581 ggtggcaatt tcaccggtcg tcaccggcat gggggtgagc caggccgtgc cggtagagtc
    38641 gacggtgaac tcggtcacgg tagtgatctc ggtgacgccc ttgtactcct ccaccttgcc
    38701 ggtcagggtg acgcggtcgc cctgggcggc gaaatgggtc ttgtcgtaca ccttaacccc
    38761 ggaccagggg gctttggcgt cctggacata atagtagtta ttcttgaatg cgtagatttc
    38821 gccggtgacc acaccgctga tggtgacgat ctggcccttg aggggggagt cgccggaggc
    38881 ttcggtcgta aactgaatgt cacgaatcgg ggtgatcgct gcccaggagg tgcccagagc
    38941 ggcgcataac agcataacca gtagcatttt tttcaaaaga gactcctttc tcttgcttga
    39001 ttcgcccccg ccggattctg tctatttgat cacaacaaat ttgccggtct ggatttcgcc
    39061 gctctgcagg tccttgacgc tgaagaagta tagtccggtg gcgatggcct ggtcgaaggc
    39121 cgagatcaga tcccaggcgt gaagtccgcc cggcagcacc gtattgctgc caccgatgcg
    39181 ctgctgcatg agttcgacgt cctcgccctt gtaggtggcc ccgtggtgga tgaattcatc
    39241 gacgacatca ccggccaggg tgtagatgcg cacctcggc
//
LOCUS       DAOPWU010000003        33313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_1250_length_33313_cov_5.599886, whole genome shotgun sequence.
ACCESSION   DAOPWU010000003 DAOPWU010000000
VERSION     DAOPWU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 33313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33313
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1250_length_33313_cov_5.599886"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKM23_00525"
     CDS             <1..348
                     /locus_tag="PKM23_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNY89981.1"
                     /translation="RNEIRKTSLGAEESVPWSYHADPVELAGRLKAEGYQIVVLEQTD
                     ASLDFSAAVYRFPACLILGHEYTGVRDELVALADMAIDIPMRGSKHSLNVSVAFGIAA
                     YEISRRRDDIRRG"
     gene            421..624
                     /locus_tag="PKM23_00530"
     CDS             421..624
                     /locus_tag="PKM23_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89982.1"
                     /translation="MALLLRERFEKLGPFGKRGLIYTLVGLAGLIAQLVRFGTRQPFG
                     ILVWALIIGIGLYYFFVLTGRRS"
     gene            652..1419
                     /gene="fabL"
                     /locus_tag="PKM23_00535"
     CDS             652..1419
                     /gene="fabL"
                     /locus_tag="PKM23_00535"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HNY89983.1"
                     /translation="MGAFTIDLSGKAALVTGGSRGIGRAIALRLAEAGADVAVNYLRQ
                     RTAAEETVRLVEKTGRRALAIRANVGEVENIPGMISEIGEAFGRLDILVSNAASGVLK
                     PALELNEHHWHWTMDINAGTLLPLTQHALPLMEAHGGHIIAVSSLGSVRAIPNYAAVG
                     ASKAALESLIRHLAVELAARNIHVNAVSAGVVDTDALKHFPNRDQLLETSRQKTPSGR
                     LTTPEDVADVVLMLCSSLAQQIHGQTIVVDGGYAIMG"
     gene            complement(1553..4117)
                     /gene="glgP"
                     /locus_tag="PKM23_00540"
     CDS             complement(1553..4117)
                     /gene="glgP"
                     /locus_tag="PKM23_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNY89984.1"
                     /translation="MAVSIQKYKVVPSLPVELNALRDLAYNLYWTWDYDLRNLFRRLD
                     RDLWESSGHNPVLLLGSIDQARLEARAKDDGYLAHLQRTRTALENYLSQKSWFQKKYP
                     QQKNFRIAYFSMEYGLNEGLPLYSGGLGVLSADHLKSASDLGIPLVGVGLLYQKGYFH
                     QYLSKDGWQQENYPENDFYNMPVKQECDTNGKPITITVEILQRTVHVAIWCAQVGRVP
                     LYLLDTNLAENSPEDQDITDSLYGGEEEMRIKQEIVLGIGGLRALERLGLRPDICHMN
                     EGHSAFMALERVRLLMQESGLTFREAQEANRNSTVFTSHTPVPAGIDEFDLGLVDRYI
                     GGYYGALQISSEQFHRLGGIGRPHNSGKFNMAIFAINMSSASNGVSKLHGEVARRMWQ
                     HLWPEIPESEVPIGHVTNGVHLPTWISEDMAELFQRYLGPRWNNIGSEDSVWDRIQQI
                     PDEELWRTHERRRERLVAFARGRLRRQLENANARPEDVAKADEVLDPQALTIGFARRF
                     ASYKRAKLIFQDLDRLKAILNNKERPVQFIFAGKAHPRDNIGKGIIRDIVNIIRKEEF
                     RSSIVFIEDYDMEVAASMVRGVDVWLNTPIRPREASGTSGMKASLNGALNLSILDGWW
                     DEAYKNTVGWGIGRGEEFSDPEEQDLFEVESLYSILEQEIVPLFYERSSNNLPRKWVA
                     MMKSSIQTLSPFFNTNRMVRDYFEQYYLPAQLRWQKLSRNKMQLVKEVAAWKERVQSL
                     WGKVHILKVDSEEGGEVRVGGRLAISAMVNCDGLKPEELRVEVYYGPLDSDEEIVDGQ
                     STVMEQVEQVKKNVYRFEATIPCETTGQHGYSIRIMPFHPEMAHSFDSGLITWLQE"
     gene            4565..5974
                     /locus_tag="PKM23_00545"
     CDS             4565..5974
                     /locus_tag="PKM23_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY89985.1"
                     /translation="MNSSSQHPILLVVLALLVLASGLFAAAGDTLSVGNSAGLPGVTG
                     YTIPVNLRNVTTLKGLLFKIKDVPDSLEVTNVVPTGRASGFRAEWTTVGGTVKILMFP
                     TITTTPLITAGNGTIANLTVSVKPEAVRGAKATVTPDSVRASNNVQQAVTVYVKSGYF
                     WYGIKGDIKADGAVDLFDVLRLIDIVLGRAPAATEYERWASDLNLDGQIDVADITALI
                     DLAVAATPRMDDSGSLSDYAGNAELTISALPRNITGSVNVPVQLRSTAPVSGLQFAFK
                     LDSRRFLVSKPRLTEMSRNMSVAIHADGDEVRVMLYSLSGDPLPLNEGMVLQIPVTIA
                     EALNADEKLEIRSALAGTMGGGRLQTQIGSGMEGVAAAPESFALMQNNPNPFNMSTKI
                     TYEVPALRQGAANIKLMVYNAQGQEVRTLVNERRSTGRYTVNWDGRDNAGNYVSSGVY
                     FYKMTADNVVITKKLAVTK"
     gene            6062..7798
                     /locus_tag="PKM23_00550"
     CDS             6062..7798
                     /locus_tag="PKM23_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89986.1"
                     /translation="MAALVAPGLAQVVDDFNRTSLGSNWTADPEYIISSNTLDNSTTA
                     TGWTFLAVYNAAANPSQVQFTWAGSANANGINAGGFAMLLDAASVTANGLLIFRRDLT
                     LEINPIVNGNVDRTIPVATAAASLPAPGPGSVIKIGFRTDATKAYFDYYINGTLDGTL
                     SYLRSSRPAIPSTYYAGVSLYGNMQNNIDDFTVWAQGITVTSPNGGENWLARSIHAIT
                     WSSNSFSGNVAIDYSLDAGSTWTVITASTANTGSYTWTLPAAPSTSARVRVRDAADGN
                     PADVSNANFTIAPETESITVLSPNGGENWEVNASHNITWSAAASIPAVRIYYSADNGA
                     NWTMIVASTTNDGSYTWTTPSVTTTRGLIRIADAADGLPLDESDAPFSILTLATLYVR
                     ETSGQPGSTNNLVAIGLDNITPVRGISFLLMDSPAYLTAMNVLPTGRASDFTVTKTDN
                     GTAVSILLVSLAGNSIPAGSGPICLISYDVSGAAPLGEFSSLDFSTVKVSDDSSRAIV
                     PELLPGRFWFVKIGDMDNSGVVDLLDINRAADIVLGREPAATDNEMMIGDLDHDGDID
                     LFDLLTIWELVY"
     gene            7871..9964
                     /locus_tag="PKM23_00555"
     CDS             7871..9964
                     /locus_tag="PKM23_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY89987.1"
                     /translation="MKRWFFLLLSGVMLLAVPQLQAQVTTYTDNFNRTALGSDWHVDA
                     EWYQIVSGALDNTLTVASWDYYAIYLALTNPTEVSYTFISTGDLEGANSAGHIMRYDL
                     VSKSGYFIYRRYGDLTLHPIIGGIIQRNAITIASVAPTQPYPKPGDVMKVVATSDAGG
                     HHFTLYINGRLDGKLTDAAKSYGNGTTLYGGIALYGQRANNIDDFTVKGTPPPTPVET
                     IGVTSPNGGEILYIGSNHAITWSSSNVTNNVKIELSTNGGTTYSLLTTVPPTPASWNW
                     TVPNTPSTTCRIRIADAVDNVPLDISNANFTITTPPDLMEVTSPNGGEDIYSGTTIPI
                     TWTTTLVGGSVKIEYSTDGGSTFNIINASAPNTGSYSWLVPALSSPTCRIRVSGAPSG
                     VPSDMSNSDFTMHITPPDLTVIRPNGGDNWLVGAQQEIQWTGPDATTMPAVNILYSVD
                     GGGTWTTAAANELNDGAYTWTVPYDVTTQALIRIEDASDADPFDQSNAFFSISTLVRL
                     LVKDSSGQPGSTGNKVSIVLDNLINVRGLSFRVTDSPNLLTAMTVTPINRALSFSVTK
                     VDNGTHVTIYMVSMSAGVITVGSGPIATISYDVSASAPVGDYAELSLSEVTVADANSL
                     PVVPDLVDGKFFFVMKGDILADGVVNDLDLERAIELLLKTGAPMTPTELLCGDLDADG
                     DFDLIDFMNIWEMIY"
     gene            9990..10781
                     /locus_tag="PKM23_00560"
     CDS             9990..10781
                     /locus_tag="PKM23_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY89988.1"
                     /translation="MKAWMRMGLGLVFGLVSLSAAAEAVLQLGQGGGQVGSQHNVVQI
                     SMANDTTVIALQLEIADVPDFIKPDSVWTADRSAGFTVAWHEDSLSVLHILVLTLDKS
                     AAIEPGSGPVLNIGYTVLPGAESFNAIDIIFYAQPKVIAPGSVRVPALSIAGKFTVGG
                     TAVESRGGQQPVRFGLEQNFPNPFNPSTRIVFHLPGNGHALLEVYNVLGQMIRTLVDG
                     ELAAGPHEAIWDGLTDRGGQAAGGIYFYRLRSGGQSEYRRMAYMR"
     gene            complement(10892..11461)
                     /locus_tag="PKM23_00565"
     CDS             complement(10892..11461)
                     /locus_tag="PKM23_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HNY89989.1"
                     /translation="MIVVEWTTLITGLIVFLARVTDVTLGTVRTIAIVHGRTKIAFML
                     GFVEVSVWLLVVAKVLAEVSAQPILTVFYAAGFSTGNVVGILLERRIAFGNTVLRVIS
                     AEEGDRIAELLRAAGYAVTSFPGEGLNGPVSMLVVVFPRKELQDVLTLVKKISPQAFY
                     ITEQAGSVSKFYRPTLQPPTGWRAVIKKK"
     gene            complement(11534..13147)
                     /locus_tag="PKM23_00570"
     CDS             complement(11534..13147)
                     /locus_tag="PKM23_00570"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNY89990.1"
                     /translation="MKRLLSLVLSLIIMTSAAEACTNFMVTPGASADSSSMITYSADS
                     HTLYGELLYLPAARHIEGTLINVYDWDSGSYLGQIKQVAETYAVVGNMNEHQLAIGET
                     TFGGRSELHNPKGGLDYGSLMYLALQRAKTAREAIRVMTSLVEEYGYCSGGESFSISD
                     PREVWIMEMIGKGEGQPGALWVARRVPDGYICGHANQARIRQFPLNDPQNSLYARDVI
                     SFAREKGYYKGSDKEFSFADTYAPLDFGALRFCEARVWAGFRRAAPSLKLPSDYARGI
                     PGSTPFPLWIKPDKKLSVRDVMELMRDHYEGTELDMTQDVGAGPYGLPYRWRPMEWKV
                     DSVKYFHERATSTQQTGFSFVAQSRAALPDPIGGILWFGVDDTYCTVYVPMYCGINRI
                     APSFAVGAGSFDAFSWNSAFWVFNFVSNYTYSRYSDMIKEVQIVQRELEGRFIAQVPQ
                     IDRAALALYKTSPQQARDYLSGFSVSAGEETVARWKKLGEFLIWKFLDGNLRDEHGGV
                     QHPPYPEAWYRTIAGQTGERYQEIPWPQK"
     gene            complement(13214..14398)
                     /locus_tag="PKM23_00575"
     CDS             complement(13214..14398)
                     /locus_tag="PKM23_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNY89991.1"
                     /translation="MKYETLFADRTSTMGANIIREILKVVGQPGMISLAGGYPAPQSY
                     PFEVIKQLSTAVLEKYGTKALQYDMTEGFLPLREALVPYLAQKGIETTAAGICITSGS
                     QGLLDALGKVLITPGDAVAVEGPTYLGALSAFNPYGPRYVCAAMDDEGILPDALERIL
                     AAHAVKFVYLVPTFQNPTGRSLGVARRHQIAELLDKYDVLLCEDDPYSDLRYRGEALP
                     TMYSLIPERTVYTTTLSKVFAPGLRIGICVAPEPVRKWLVLAKQGVDLNTSTFNQAMA
                     AEYLLGGYLEKQIPKIIDLYKPKQEVMLNALDRYFPREWHYNKPEGGMFIWVEGPKGT
                     DAEKLYWKCIERKAAFVPGKFFFPCEGDGLETMRLNYTMSSEEEIERGIKIIGDTIQE
                     GF"
     gene            14670..17027
                     /gene="pbpC"
                     /locus_tag="PKM23_00580"
     CDS             14670..17027
                     /gene="pbpC"
                     /locus_tag="PKM23_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNY89992.1"
                     /translation="MKHKRLIYGAAALGILLALLVVIPPSPSRLDSSRLHSRRIYDDR
                     GRLLREVLSDEEGRGMWIPLAEMGPDVAAAVVAIEDKRFYRHPGIDLLALARSTWLNL
                     RAGEVVAGGSTLTQQLARQLYRLPRRWYAKPAEALLALRLELWLSKEEILEHYLNRAP
                     FGNQLFGVASASRIYFQQSAVELSLAEAAFLAGLPQSPSGYNPYTGLARARERQLRVL
                     AAMRRQGVIPEERYRAACAQPIEVALRKSVFAAPHFCQMVLEKAETDRFEIHTTLNSA
                     MQSRIEKLVEGHLAQLTAHHVTNAAVLVLDNSSGAVKAWVGSRDFFDALYQGQVDGVR
                     SLRQPGSAIKPFTYALALENGFTAASLLADMESYAGEEGGGTLVHNYDEKYHGPVRIR
                     TALACSYNVATVRLLEALGVDLLLERLRQAGFSSLQKPASFYGPGLTLGNGEVSLSEL
                     TNAYRTLANGGLVRPFHFLRDEAAAAGMADGESASGEWQGERIFSRSAAFIITDILSD
                     PHARAPAFGLGGPLHLPFPCAAKTGTTKDFRDNWTVGYTTVYTVGVWVGNFDGQPMER
                     ISGITGAAPLFRDVMLELHAGFDPAPFPLPPGVVQRAICPVSGQRPSTACPGSADEWF
                     IAGTEPRSECSVHRLVALDRRSGQPASPATPRAEIRETLYEVWPPEYRAWMAANDLPM
                     PPAALSVPGEAEAPRLVITFPKEGDILRIDPVLRREFQTILLEAVVPEGISEVEWMIG
                     DSTLARLHAPYRLRWPLTPGSHRLVVRGRGRNGTVSSDPVTFRVL"
     gene            17098..17556
                     /locus_tag="PKM23_00585"
     CDS             17098..17556
                     /locus_tag="PKM23_00585"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNY89993.1"
                     /translation="MRETGVVLSSSGRVAQVRILRSPQCAGCHGCIELGEPQERLIEV
                     ENDLDAPAGAVVECEIASGQVIGHTLLVFFFPLFGMAAGYYAAAGRLSGRPAAEGIGV
                     AGAFAGLLLAFLLVRLVDKHWGRKHPTTGRICGYAGAGTEEENSFSCPQH"
     gene            17586..21572
                     /gene="porU"
                     /locus_tag="PKM23_00590"
     CDS             17586..21572
                     /gene="porU"
                     /locus_tag="PKM23_00590"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNY89994.1"
                     /translation="MRRYLILVLTVILPAALGAAVQQGVRLLSGSNGQLVLEFVPEHW
                     SIDSVRVDGRFYQSYLFAGAELTGAPGAPQLPARTVVAGIPPAGGVSLRILSAEYETR
                     TSSVLPMGRVVRDGLLTRTEYQQDPVEYGRQDFGPGIVRISEPADFRGQRAVNVTFYP
                     VQAAPGARQVRLYRRIVAELSFASPATGSSASAAVGAAADGLLQNLLINPEQARAWRT
                     SEPARLAKSHRALFPGDNWYKIIIRGDGSGGKEGLYKLTAAALSQAGVPIASVDPSTL
                     QLFNNGGRELPENISAARPDSLLENAILVNGESDGRLDSGDYILFYGRPLEGEYYSSS
                     DGKYRHYINRFGYENVYWLTFGLNKGKRMQSRTSLPAFGEAESSFRDLAYNEDERANI
                     YKSGSDWLGFELARDKNNYSQSFSLPGAVPQASAVFRYQVAATTSGSHLFKMNANGNS
                     LGQFNLAGQLSAYTPRQTEFTAAGVLLEGSNTVGIEYGAASDISQAYVDWVEVEYQRR
                     FQPVNDQLIFNSPLREGAALYEIGNFERTEMAVYDITVFNDVSVIAGTQLVGSKIRFS
                     DQVTTQSPRRYLALTPAAYMAVTEIRRDAASDLRRPRDVDYIIITHDNFYQQAMQLES
                     LREDWNSSDRLETEVVRISDVYDEFSWGLTDPVAVRDFLAYAYANWGAPGYVLLFGDG
                     HYDFRNIYRYGTPNLVPPYESPETSEIASRTSDDWYTYFKGNNNGMQMAIGRLPVQTV
                     EEAQGVVTKLIEYETKALAEEWRKTVTVVGDDELVSGGKGEELEHTRQAEWLAERYVP
                     AALNVEKIYLMEYPAVRTASISGVTKPQASAAFIERVNQGSLIVNFIGHGADELLTHE
                     NIFNLSTDFDKIQNARRYALWIASTCEFAYWDQPQKQSFAEYLVNAPARGAIGMISST
                     RLVYSSENATLNYNFFNYLFNGYEASGRVARVGDALMLAKRGSSASQLNNEKFVLLGD
                     PAMRLAAPRYRASVDTLSPGATLQALTHYTVKGSVARAGASWQDFDGTLLLRVFDARK
                     PRTYTSEGGASINYILPGNTIFRGTALSGAGRFSAQFIVPKDISYGGSDGRISLYFWS
                     PEGDGTGYMNNLTVDGSAMGLVDREGPLVKVHFGNPSFASGDYTTRNPVLHVQISDSL
                     SGVNIAGDIGHQIIMNLDEGEGKNITEYFQYYKGSYTSGELQYPLVQLTPGRHSLSLK
                     AWDNSNNSSIAEIEFMVVAESELTLSNLLNYPNPMSDRTQFTFEVSRNAEVALQLYTV
                     AGRLVRKFPAMQAEVGFNVYPEVWNGMDAEGDPVANGVYLYRVHARSAGPEGAAETDA
                     VGKVIVAR"
     gene            complement(21597..23873)
                     /locus_tag="PKM23_00595"
     CDS             complement(21597..23873)
                     /locus_tag="PKM23_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNY89995.1"
                     /translation="MGSAMESAFRLVIIEEEAQQAEIIAREFKNAGLLGEWVRVNSAK
                     GFTQALAGKPDLIIANYFLPAFDALQALAHLQEHHLDIPLIVLSGSLDSEIAVACMKA
                     GAADVIHSDHIFRLAASAREAVEKKEHERRTRRIEKALRFTKFAVDHAAVAIFWLNHE
                     GRFALANEAGLKMLGYTMPELSTLSISAVDPNLVPEKWKALLKSMTSRHSATFESRLS
                     TKSGQIVPAELTLNDYQLDSQDFVLCFAHDISDRKRSEEEYQRLVIAIEQAAESIMIT
                     DVENRIQYVNPAFERVTGYDREEVLGKKPSILHSGKHDAAFYREIWTTLMSGKSWHGH
                     FVNKRKDGTLFEEEATISPIKEKGGQIISYVAVKRDVTHEIDLQKQVYHAEKMDAIGR
                     LAGGVAHDFNNLLTIINGYSELMIKSIMPSDPLYGHVQQIIKACARASTHTKQLLAFS
                     RRTPMEQKVIDLNATLIDMVSVLKRLVNENIDLVTRFKEGTGNIKADPAEVEQIIVNL
                     VVNARDAMPRGGKLVIETDRVSLDQKYCRSHIDATPGEYMLLSVQDNGIGMDEETKAR
                     IFEPFFTTKEQGTGLGLPTVFGIVRQNKGHVECYSEKGKGTLFKIFLPLVAEAAAETD
                     LRAVYDTLPRGKETILLVEDEEDVRVLTSRMLQLQGYKVIEAALAGEALIASERASGP
                     IDLLLTDVVMPQMGGAELAERLRKTNPGLKVLYMSGYASDVLMAHENVDRETVAFIAK
                     PFTLENITRKVRDVLDQN"
     gene            complement(23889..24449)
                     /gene="lspA"
                     /locus_tag="PKM23_00600"
     CDS             complement(23889..24449)
                     /gene="lspA"
                     /locus_tag="PKM23_00600"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNY89996.1"
                     /translation="MKSYRILWVSLFLFVLDQATKWVVASNMRLHESIPVLGDFFRLT
                     YVENPGMAFGIRISNNLFFTVFAVVASIVIMLYLFKLKERQRWARLAMTVILGGALGN
                     LTDRILRGRVVDFLDFEFFDIHIPAFKLFSIPFAGYSLERWPVFNVADIALSVGMVML
                     LIYILFLEEDETQLQAEAADTSEMIR"
     gene            complement(24452..24829)
                     /locus_tag="PKM23_00605"
     CDS             complement(24452..24829)
                     /locus_tag="PKM23_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNY89997.1"
                     /translation="MTKEQLAYLKKLIMEKRDRMIMELDHMKSSGLNTSMKETSGDHS
                     SYSFHMADQGTDTMDREQQYFFAARDGNLIYHLNLALERIEKGEFGYCVQCGKEISFE
                     RLEAAPHARLCIECKSKEEMSKR"
     gene            complement(24861..28004)
                     /gene="ileS"
                     /locus_tag="PKM23_00610"
     CDS             complement(24861..28004)
                     /gene="ileS"
                     /locus_tag="PKM23_00610"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNY89998.1"
                     /translation="MYEPLPEKLNFPEIEKRVLEFWQEQRIFERSVSERDENNPFVFF
                     EGPPTANGRPGIHHIISRTVKDAVCRLRTMQGFRVERKAGWDTHGLPVEIEVEKSLGL
                     DGKDQVIEYGIDKFNAKCRESVWAYLQEWDEITRRIGFWVDLKNPYITYTNDYIESVW
                     WILKEFWKKGLIYQGHKILPYCPHCETPLSSHEVSQGYEEVRDPSVYVRARVKGQKET
                     YFLVWTTTPWTLISNVALALHPEIDYVKVKHQDQFLILAKARLEVLEGDYEIVGEFSG
                     EKLAGLEYEPIYDFVKPDKRSHYVVLGDFVTTGDGTGIVHIAPAFGEDDYQLGKKYDL
                     PVLQPVDKSGKFTAEVTPWAGLFVKEADKGIIRELKERGVLYRSEPITHSYPHCWRCK
                     SPLLYYARKSWYIQTTAIKERLIANNKKIDWYPKEVGEGRFGEWLENNIDWSLSRDRF
                     WGTPLPVWRCTTCDAQECIGSIAELREKAAHEVPADLELHKPMVDRIPLKCPACGGVM
                     ERVPEVIDCWFDSGSMPYAQWHYPFENKDKFAKSFPADFISEGVDQTRGWFYSLLVIG
                     AFLFDQPAYKSCVSIELILDKEGQKMSKTRGNAVAPIEFLDNQGADALRWYLLTVSPP
                     WLPTRFDPEGVRDVIRKFLGTLANTYSFFALYANIDQFSYAATPRIPVAERSELDRWL
                     CSSLTALTKRVNDHLARYDLTRASRAISDFVIDDLSNWYVRRSRRRFWKAEMGEDKLA
                     AFQTLYEALFTVSRLMAPMAPFIADEIYRKLNDGGAGHADSVHLASYPRAGEAPADFR
                     DEKLEVRMDLVRRIVFLGRSLRNEAGTRVRQPLAAILVMASDEKHHRMIEGMSGLILD
                     ELNVKSLQFVSDQKALLSRRAEPIFKNLGPKFGKNVNKAAEAIRAFSEADIDSLLASG
                     SKQMVIEGHEARITTDDITIQTENREGLVAASEVDLTVALDLNLTEALRCEGLAREFV
                     NRVQNMRKEAGFEVTDRINIWLQASPELEQAVASQSEYITSETLCESLRLGDTGGTFQ
                     KELKIEDQLLKAGISRL"
     gene            complement(28060..28923)
                     /gene="mtnP"
                     /locus_tag="PKM23_00615"
     CDS             complement(28060..28923)
                     /gene="mtnP"
                     /locus_tag="PKM23_00615"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNY89999.1"
                     /translation="MPRIGIIGGSGLYQIEGLEEIREVRVETPFGDPSDLLILGRLEG
                     REVLFLPRHGRGHRILPAEVNNRANIWALKSLDTAWIISVSAVGSLKPGLKPQDLVIV
                     DQFVDRTNQARPNTFFGGGIAAHITFAHPLCESVGQILYTAAREMGASIHWGGTYVNM
                     EGPAFSTLAESQLYRSWGMDVVGMTQMNEARLAREAEICYATLAMVTDYDCWWEAETG
                     QTVSVELVVENLRQSSALAKELIRRSIASFPWQPACACQEALAGAIITERGAWPQESI
                     TRLGPILKKYL"
     gene            complement(28937..29749)
                     /locus_tag="PKM23_00620"
     CDS             complement(28937..29749)
                     /locus_tag="PKM23_00620"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNY90000.1"
                     /translation="MKEQVQEAAAWIRTRDARAFDLAIVLGTGLGALADEIGEARVIP
                     YDEIPHFPASTVKFHAGRLVLGELEGKTLLAMQGRFHGYEGYPMRQITLPIRVMRALG
                     IQRLILTNAAGGIRPELTPGSIGLIRDHINLMGTNPLIGPYDPELGVRFPDMSEPYSA
                     VLRGLARRIAAEQGVTVHDTIFGAVIGPAFETEAEIRMLQILGVDTVGMSVVPETLVA
                     RQLGMEVLALTAVTDQSLPGAMVSVSHEAVNHVAAELGPRFRALLRAIIRAI"
     gene            complement(29787..30686)
                     /locus_tag="PKM23_00625"
     CDS             complement(29787..30686)
                     /locus_tag="PKM23_00625"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNY90001.1"
                     /translation="MKLTPLDIRKQEFKKSMRGFDPEEVEAFLIMVADELEIVLREKN
                     QQSDELIKLRTQLRDYQQVEHTLRETLMKATSTVEESRFNSLREAELRIHEAELQAER
                     IIENAKEELQELRSEINLLRAQKESFSRRLRHLLESQIELLDVLGIDDAMIKQSSNTE
                     EPQGPALRGRLPRPVLPAEKENPPAPGSEQPHAPVSAQAQTHGAAPTASGIAPAGAAP
                     AAPAAPSSSFNIPPAGSSGPAEAETGSFFARSEGPRIIRGERRFDEPTGMQFSDESEQ
                     ELPEKNGEEEQGRENNLSDQIVI"
     gene            complement(30702..31382)
                     /locus_tag="PKM23_00630"
     CDS             complement(30702..31382)
                     /locus_tag="PKM23_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNY90002.1"
                     /translation="MAIFDNLKYVTERIQAAALHSGRQAGEVALLAVTKSVPAERMRE
                     AAALGLTLIGENRVQEAWTKFQSLADLPLSWHMIGHLQSNKVKRALQFATLIESVDSL
                     HLAREIEREAAAQGKIIECFLEVNTSGESQKFGIAPGEAEALAGDLAGLPHLHLTGLM
                     TIGALSTDSQRIRSCFSQLRKVQERLNASGLGISQLSMGMSDDFEIAIEEGATIIRLG
                     RALFGERN"
     gene            31563..33155
                     /locus_tag="PKM23_00635"
     CDS             31563..33155
                     /locus_tag="PKM23_00635"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNY90003.1"
                     /translation="MILLIPLTTQPLLAGRVVTVGREGPPQYDYSALQQVHSWLTQLP
                     GGVSEPLTIRLYDSEFPEKTLVWEVSGTEKAPILIQGVGDVWFSGTRVKGEAYGRPVL
                     ILSWVSHITLENIGFRDFDGRQESRVGLKITGKGKNRIQSCRFSHFFGSRSEAAISLY
                     NEASGNLIEQCEFDSLGDDLYMHAIYLNKNCRHNIIRNNTITYCSGEAFMVRNGSDRN
                     WFDGNTVVRCGGVAYYGEWYDQSPGSNEKPSRGNRITRASGQGPAAKSSGYMRSGYIR
                     KIRALNWSRYKWPNYKEDWTDSTLAYYMPDADKNRLVTVADDMEIRVKAEIDATPAYL
                     RLGKSPIYHAQVVPEGELEGARVELSFADTTNRWGGDERYLREIRLHDTSSRPRRFTT
                     PLQLRTLEGDLIHELYPVRELRGKTVEPAVIAVEGAGQKITFTLTRMQGDYDLFILPQ
                     KGSLSVEKLRAHFDDEDGGGDFFAENALSSGFLSLGKAASRGQGSRDEKVSLTLAAGR
                     SFWMVISCKPGAKSGCTWAARP"
BASE COUNT         6344 a         9743 c        10458 g         6768 t
ORIGIN      
        1 cgcaacgaga tccgcaaaac cagtctcggc gccgaggaga gcgtgccctg gagctatcat
       61 gcggatccgg ttgaactggc cgggcgtctg aaagcggagg gataccagat cgtcgtgctc
      121 gaacagaccg acgccagcct cgatttcagc gccgccgtct accgtttccc ggcctgtctg
      181 atcctcggtc acgaatatac gggcgtacgc gatgaactgg tcgctctcgc cgacatggcg
      241 atcgatatcc cgatgcgagg cagcaaacat tcgctcaacg tctcggttgc ctttggcatc
      301 gcggcctatg aaatatcgcg ccgccgggat gatatccgcc ggggatgatg ttctaattgc
      361 catgccgggc ggagtgcaca cacctgaacc tggtcgggag ctgaatagag gagaacgaag
      421 atggcgctgt tattgaggga gcgtttcgag aagctggggc cctttggcaa gagaggcttg
      481 atctataccc tggtgggatt ggccgggctc atcgcccagc tggtccggtt cggcacgcgc
      541 cagcccttcg ggattctcgt ctgggccctt atcatcggca tcggcctcta ttatttcttc
      601 gttctgactg gacgccggtc ctgattcaca atccattcgc gggagaagag tatgggagcc
      661 tttaccattg atttaagcgg caaggccgct ctggtcaccg gcggttcgcg cggcatcggc
      721 cgggcgatcg ccctgcgcct ggcggaagca ggggccgacg tggccgtcaa ctatttgcgt
      781 cagcgcacgg ccgccgaaga gacagtgcgg ctggttgaga agaccggacg ccgcgccctc
      841 gccattcgcg ccaacgtggg cgaggtagag aatatcccgg ggatgatcag cgagatcggc
      901 gaggccttcg gacgcctcga catcctggtc agcaacgccg cttccggcgt gctcaaacca
      961 gccctcgagc tgaacgaaca tcactggcac tggaccatgg acatcaatgc cgggacgctc
     1021 ctgccgctga cccagcacgc cctgcccctg atggaggctc acggcggcca tatcatcgcg
     1081 gtgtcgagcc tcggctctgt gcgcgccatt cccaattacg ccgccgtcgg ggcctcaaag
     1141 gcggcgctcg agtccctcat ccgccatctg gcggtggagc tggccgcgcg caatatccat
     1201 gtcaatgccg tctcggccgg ggtggtcgat acagacgccc tgaagcactt tcccaaccgc
     1261 gatcagctgc tcgaaaccag ccgccagaaa accccctccg gcaggctgac cacgcctgaa
     1321 gatgtggctg atgttgtgct gatgctctgc tcctctctgg cgcagcagat tcacggtcag
     1381 accatcgtcg tcgacggcgg ctatgccatc atgggttgat tcggggagct tctatccctg
     1441 aggcggggcg atcgcgataa aacaaaggcg cgctcatcat ttgaggtgat gagcgcgcct
     1501 tcctgtttcg gacgggagca tcctggctga tcacaacccg gagaattttt tgctactcct
     1561 gcagccaggt gatcaggccg ctgtcgaagg agtgggccat ctccggatga aagggcatga
     1621 tgcggatgga gtaaccgtgc tggccggtgg tttcgcatgg gatggtcgcc tcgaagcgat
     1681 aaacattctt cttgacctgt tcgacctgtt ccatgacggt gctctggccg tcgacaattt
     1741 cctcatccga atccagcggt ccgtaataga cctccacgcg cagttcttcc ggtttgaggc
     1801 cgtcgcagtt gaccatggcg gagatagcca gtcggccgcc gacgcggacc tcgcccccct
     1861 cctcggaatc gaccttgagg atatggacct tgccccagag gctttgcacc cgttctttcc
     1921 aggccgccac ctccttgacc agctgcatct tgttgcggct cagtttctgc cagcgcagct
     1981 gggcgggcag atagtactgc tcaaagtagt cgcggaccat gcggttggtg ttaaagaagg
     2041 ggctgagggt ctggatgctg ctcttcatca tggcgaccca tttgcgcggc agattgttgc
     2101 tgctgcgttc gtaaaagagc gggacgatct cctgttcgag gatggagtag agggactcca
     2161 cctcgaagag atcctgttct tcgggatcgg agaattcctc accgcggccg atgccccagc
     2221 ccactgtatt tttataggct tcatcccacc agccgtcgag gatgctgagg ttgagagccc
     2281 cgttcaggct ggccttcatg ccgctggtgc cgctggcttc gcggggacgg atgggggtgt
     2341 tgagccagac atcgaccccg cgcaccatgc tggcggcgac ctccatgtca tagtcttcga
     2401 tgaagacgat gctgctgcgg aactcttcct tgcggatgat attgacaatg tcgcggatga
     2461 tgcccttgcc gatattgtcg cgtggatggg ccttgccggc gaatatgaac tggaccgggc
     2521 gttccttgtt gttgaggatg gccttgagcc ggtcgaggtc ctggaagatg agtttggcgc
     2581 gtttgtaaga ggcgaagcgg cgtgcaaaac caatggtcag ggcctgggga tcgagcactt
     2641 catcggcttt ggcgacatcc tcgggccggg cgttggcgtt ttcgagctgg cggcgcaggc
     2701 ggccgcgggc gaaggcgacc agccgttcgc ggcggcgttc gtgggtgcgc cagagctctt
     2761 catcgggaat ctgctggatg cggtcccaga ccgagtcctc ggagccgatg ttgttccagc
     2821 gcgggccgag atagcgctgg aagagttcgg ccatgtcctc ggagatccag gtcggcaggt
     2881 ggacgccatt ggtgacatgg ccgatgggga cctcgctctc ggggatttca ggccagaggt
     2941 gctgccacat gcggcgggcg acctcgccat ggagcttgct gacgccgttg ctggcgctgg
     3001 acatgttgat cgcaaagatc gccatgttga atttgccgct gttatggggc cggccgatgc
     3061 cgccgaggcg gtggaactgc tctgaggaga tctgaagggc gccgtaatag ccgccgatgt
     3121 agcggtcgac caggccgaga tcgaactcat cgatgccagc gggcacaggg gtatggctgg
     3181 tgaagaccgt gctgttgcgg ttggcctcct gagcctcccg gaaggtgagc cccgactcct
     3241 gcatgaggag gcggacgcgt tccagggcca tgaaggctga atggccctcg ttcatatgac
     3301 agatatcagg ccggaggccg aggcgttcga gggcgcgcaa accgccgatg ccgaggacga
     3361 tctcctgctt gatgcgcatt tcctcctcgc cgccgtaaag ggagtcggtg atatcctgat
     3421 cctcagggct gttctcggcg agattggtgt cgaggaggta gagggggacg cggccgacct
     3481 gggcgcacca gatggcgaca tggaccgtgc gctgcaggat ttcgaccgta atggtgatcg
     3541 gcttgccgtt ggtatcgcat tcctgcttga cgggcatgtt gtaaaagtcg ttctccggat
     3601 agttctcctg ctgccagccg tctttggaga ggtactggtg aaaatagccc ttctggtaga
     3661 gcagtccgac accgacgagg gggatgccca ggtcgctggc cgatttaagg tgatcggcgg
     3721 agaggacgcc gaggccgccg gagtagagag gcagcccttc gttcaggccg tattccatcg
     3781 agaaataggc gatgcggaag tttttctgct gggggtattt tttctgaaac caggattttt
     3841 gcgagagata gttctccagt gcggtgcggg tgcgctggag atgggcgaga tagccgtcat
     3901 ccttggcgcg cgcttcgaga cgggcctggt cgatgctgcc gagcaggagc acgggattgt
     3961 gcccggagct ctcccagaga tcgcggtcga gacggcggaa gagattgcgc aggtcatagt
     4021 cccaggtcca gtagaggttg taggcgaggt cgcgcagggc gttgagttcc actggcagcg
     4081 agggtaccac cttgtacttt tggatggaga cggccataat aggatcctta taaaaaaaag
     4141 ccggtcagaa aatatttcat aaagatagtg aaaaaagcgc ttccaatcaa gggaaaatgc
     4201 gcccccgccg cgctgccggg ggttttgcag ggggacaagt ggcacgactg ttgcaaaaga
     4261 ggagatggaa gggaaaagga gtgcgcatag gacgccttct ccctccggcc ggaatgaatc
     4321 ggcaaagagg ttactcacag aaaaacaatg gttagaagga taagggtcgt cgggtggatg
     4381 aggtgaatgc acagaacagg ttcaacgggt caacgccggt tctgtattgc cctgagttag
     4441 cgtatcagag agagacactt gggcatccat tgcactgcac ggcagcattg ttcggcaccc
     4501 cgctcctcac tctcgcaagc atggctattc aacaggttcc aactttcaat gtatggagga
     4561 agtgatgaat tcttcatctc agcatcccat cctgctggtg gtattggccc tcttggttct
     4621 ggcctccggc cttttcgccg cggccggcga taccttgtcg gtggggaaca gcgccggact
     4681 gccgggtgtg accggctata ccataccggt caacctgcgg aatgtcacca ccctcaaggg
     4741 gctgctcttc aagatcaagg atgtgcccga ttcactggag gtaaccaatg tggtgccgac
     4801 cggaagggcc tcgggatttc gcgcggagtg gacgacggtg ggcgggacgg tgaaaatcct
     4861 catgttcccg accatcacca ccacccccct catcacagcc ggtaacggga ctatcgccaa
     4921 cctgacggtt tcggtgaaac ccgaagcggt gcgcggggcc aaggcgacgg tcacgcccga
     4981 cagcgtccgg gcctccaaca acgttcagca ggcggtgacg gtctacgtca agagcggcta
     5041 tttctggtac gggatcaagg gcgatatcaa ggccgacgga gcggtcgacc tcttcgacgt
     5101 tctgcgtctt atagacatcg tcctcggccg cgcgccggct gccacggaat atgaaagatg
     5161 ggccagtgat ctcaacctgg atggccagat cgatgtcgcg gacatcaccg cactgatcga
     5221 tctggcggtc gccgccactc ccaggatgga tgacagcggt tcgctctccg actatgccgg
     5281 caacgccgag ctgaccatct ccgccctgcc caggaatatt accggttcgg tcaacgtgcc
     5341 ggtacagctg cgctccacgg cccccgtcag cggcctgcag ttcgccttca agcttgattc
     5401 gcgccgtttt ctcgtctcca agcccaggct gaccgagatg agcaggaata tgtcggtggc
     5461 gattcacgcc gatggcgatg aggtgcgggt gatgctctac agtctgagcg gcgatcccct
     5521 gcccctcaat gagggcatgg tgctgcagat cccggtgacc atcgctgagg ctctgaatgc
     5581 ggatgaaaag ctggagatca ggtcggcgct ggccggcaca atgggcggcg gcagactgca
     5641 gacccagatc ggctccggaa tggaaggggt agcggcggcg cccgagtcct tcgccctgat
     5701 gcagaacaat cccaatccgt tcaacatgtc gaccaagatc acttatgagg tgcctgcgct
     5761 gcggcagggt gcggccaata tcaagctgat ggtctacaat gcccaagggc aggaagtgag
     5821 aaccctggtt aacgagagaa ggagcacggg aagatatacg gtgaactggg atggcaggga
     5881 taatgcaggc aactatgtct cttccggtgt ctacttttac aagatgactg cagacaatgt
     5941 ggtgatcacc aagaaactgg cagtcaccaa gtaagaggct tccttcgtac gggaatcact
     6001 tttaggagag gaatttaaga tgaaatcaag ccgggcgact gccttttggg cactcattat
     6061 cttggcagct ttagtcgcgc ccgggctggc gcaggtcgtg gacgatttca accggaccag
     6121 cctgggatcg aattggacag ccgatccaga atatatcatt tccagcaata ccctggacaa
     6181 cagtaccact gcgaccgggt ggacctttct ggccgtctat aatgctgcgg ccaatccctc
     6241 gcaggttcag ttcacctggg cggggagcgc caatgccaac ggcatcaacg cgggtggttt
     6301 tgccatgctc ctcgatgccg cttcggtcac agccaatggt ctgctgattt tccgtcggga
     6361 tttgaccctg gaaatcaatc cgatcgtcaa tggcaatgtt gaccgcacca tccccgtcgc
     6421 cactgcggcc gcttcgctgc cggcgccagg gccgggcagc gtcatcaaga tcggcttccg
     6481 cacggatgcg accaaggcct attttgacta ttatatcaat ggtaccctcg acggcactct
     6541 cagctatctg cgctccagcc ggccggcgat cccttcgacc tattatgcgg gtgtgagcct
     6601 ttacggcaac atgcagaaca acatcgatga tttcaccgtc tgggcccagg gcataacggt
     6661 tacctccccc aacggcgggg aaaactggct ggctcgctcg atccacgcca ttacctggag
     6721 ttcgaacagc ttttccggca atgtggcgat cgactactcc cttgatgcgg gatcgacctg
     6781 gacagtgatc accgcttcaa cagccaacac cggttcctac acctggaccc tgccggcggc
     6841 cccgagcacc agcgcgcggg tgcgcgtgcg ggatgccgcc gacggcaatc ccgccgatgt
     6901 cagcaatgcc aactttacca tcgcgcccga gaccgaatcg atcacggtgc tctcgcccaa
     6961 cggcggggag aactgggagg tcaatgcctc gcacaacatc acctggagcg cggcggcttc
     7021 gatccccgct gtgcgcattt attattcagc ggacaacggc gcgaactgga cgatgatcgt
     7081 tgccagcaca accaatgacg gctcctacac ctggaccacg ccgtcggtga cgaccacccg
     7141 cggccttatc cgcatcgccg atgccgccga cggcctgccc ctggatgaga gcgacgcccc
     7201 gttttctatc ctgaccctgg cgaccctcta tgtgcgcgaa accagcgggc agccgggatc
     7261 gaccaacaac ctggtcgcca tcggcctcga caacatcacc cctgtacgcg ggatctcctt
     7321 tctgctcatg gattcaccgg cctatctgac cgccatgaat gtcctcccca ccggccgcgc
     7381 ctccgacttt acagtcacca aaacggacaa cggcacggcg gtctccatcc tgctggtcag
     7441 tctggcgggc aactcgattc cggccggcag cggcccgatc tgcctgatct cctatgacgt
     7501 ctccggcgcc gctcccctcg gtgaattctc gagtctggat ttctccaccg tcaaggtcag
     7561 cgacgacagc agcagggcga ttgttcccga attgctgccg ggccgcttct ggttcgtcaa
     7621 gatcggggat atggacaaca gcggcgtggt tgatctgctc gatatcaacc gcgcggccga
     7681 catcgtcctg ggcagggaac cagccgcgac cgacaacgag atgatgatcg gcgaccttga
     7741 ccatgatggc gatatcgacc tgttcgatct gctgacgatc tgggaactcg tctactaatg
     7801 caacggagag ggtatggggc tccttgccct gctctgcaag aagcgcatgt attcgaacgt
     7861 gggtaggctt atgaaaagat ggttttttct cctattatcc ggtgtcatgc tcctcgctgt
     7921 tccacagctg caggcgcagg tcactactta tacggataat ttcaaccgca ccgccctggg
     7981 atccgactgg catgttgacg ctgaatggta tcagatcgtc tcgggtgcgc tggataacac
     8041 ccttacggtg gcgagctggg actattatgc catctatctg gcactgacca atccgaccga
     8101 ggtgtcatac accttcatct ccaccggcga tttggagggt gccaactcgg ccggccacat
     8161 catgcgttac gatctggtct ctaaaagcgg ctattttatc taccgccgct acggcgattt
     8221 gaccctccat cctatcatcg gcggcatcat ccagcgcaac gccataacca tcgccagcgt
     8281 agcgcccact cagccctatc ccaaaccggg cgatgtcatg aaggtggtgg cgaccagtga
     8341 cgccggcggc caccatttta ccctttatat caacggccgg ctcgacggca aactcaccga
     8401 tgccgccaag agctatggca acggaaccac tctttacggc ggcatcgccc tctatggcca
     8461 gcgcgccaac aatatcgatg atttcaccgt caaagggacg cccccgccca ctcccgttga
     8521 gaccatcggg gtgacctccc ccaacggcgg cgagattctc tatatcggtt cgaatcatgc
     8581 catcacctgg agttcgtcca acgtgaccaa caacgtcaag attgaactct ccaccaacgg
     8641 cggaactacc tattccctgc tgacgacggt gccgccgacc ccggcctcct ggaactggac
     8701 tgtgcccaac acgccctcga ccacctgccg catccgcatc gccgatgcag tcgacaacgt
     8761 cccgctggat atcagcaatg ccaattttac catcaccacc cctccggacc tcatggaggt
     8821 gacctccccc aatggcggcg aggatatcta ttccggcact accattccga tcacctggac
     8881 cacgacgctt gtgggcggat cggtcaagat agaatactcc accgacggcg gcagcacttt
     8941 caacatcatt aacgcatcag ctcccaatac aggcagctac agctggctgg tgccggcgct
     9001 gagttccccc acctgccgca tccgcgtctc gggtgctccc agcggcgtgc cgagcgatat
     9061 gagcaacagc gactttacca tgcatatcac cccgcccgat ctgacggtga tccgcccgaa
     9121 cggcggcgac aactggctgg tcggcgcgca gcaggagatc cagtggaccg gtccggatgc
     9181 cacgaccatg ccggcggtca acatcctgta ttcggtcgac gggggcggga cctggacgac
     9241 agccgccgcc aacgagctca atgacggcgc ttacacctgg accgtcccat acgatgtgac
     9301 aacacaggct ctgatccgga tcgaagatgc cagtgatgcc gatcccttcg atcaaagcaa
     9361 cgccttcttc tcgatctcca ccctggtcag gctgctggtc aaggacagca gcggccagcc
     9421 gggatccacg ggcaacaagg tatcgattgt gctcgacaat ctcatcaacg tccgcggcct
     9481 ctccttccgg gtgaccgatt cgcccaatct gctcacggcg atgacggtga cgccgatcaa
     9541 tcgcgccctg agcttctcgg tcaccaaggt cgacaatggc actcacgtca ccatctacat
     9601 ggtttccatg tccgccgggg tgatcaccgt cggcagcggc ccgatcgcca cgatcagcta
     9661 tgacgtctcg gcgagcgcac cggtgggtga ttatgccgag ctctccctct ccgaggtcac
     9721 cgtcgctgat gccaacagcc tgccggtcgt ccccgatctg gtcgacggca agttcttctt
     9781 tgtgatgaag ggggacatcc tcgccgacgg ggtggtcaat gacctcgatc tcgagcgggc
     9841 catcgagctg ctgttgaaaa ccggagcgcc gatgaccccg accgaactcc tctgcgggga
     9901 tctcgatgcg gacggggatt ttgacctcat cgatttcatg aacatttggg aaatgatcta
     9961 ttgagcgtct attttcggca ggacaggata tgaaagcatg gatgaggatg ggattggggc
    10021 tggttttcgg cctcgtctcg ctctccgcag cggctgaggc ggttcttcag cttggccagg
    10081 gcggaggcca ggtcggcagt cagcataatg tcgtccagat cagcatggcc aacgatacga
    10141 cggtcatcgc cctgcagctg gaaatcgccg acgtgcctga tttcatcaaa ccagattcag
    10201 tctggacggc ggatcgttcc gcggggttca cggtggcctg gcacgaggat tccctgagcg
    10261 tgctgcacat cctcgttttg actctggaca agagcgctgc catcgaaccg ggcagcgggc
    10321 ctgtcctcaa tattggctac accgtcctgc cgggcgcgga gagctttaac gccatcgaca
    10381 tcatctttta cgcccagccg aaagtgattg cacccggctc cgtccgggtg cccgcgcttt
    10441 ccatagctgg caagttcacc gtcggcggca cagctgtcga aagcagggga gggcagcagc
    10501 cggttcgctt tgggctggaa cagaactttc ccaatccctt caatcccagc acgcggatcg
    10561 tttttcattt gccgggaaac gggcatgccc tgctggaggt ctataatgtg ctgggtcaga
    10621 tgatcagaac cctggtcgat ggcgagctgg cggccgggcc gcacgaggcg atctgggatg
    10681 gcctcaccga ccgggggggg caggcagccg gagggatcta tttctaccgt ctgcgttcgg
    10741 gcggccaatc cgagtacaga cgtatggcct acatgcgctg aaggtatgaa catggcgcgt
    10801 tcgcgggttt cttcacgggt atgacggcgt gagggaacgc gcctcttttc aagccgcagg
    10861 gtttcctgcg gcttttttta tgggcagaac gctatttctt tttgatgacc gcgcgccagc
    10921 cggtgggggg ctgaagggtg gggcgataga acttgctgac gctgccggcc tgctcggtga
    10981 tataaaaggc ctggggtgag atctttttca ccagggtgag aacgtcctgc agctctttgc
    11041 gcgggaagac cacgaccagc atgctgacgg ggccgttaag accttcgccc ggaaaggagg
    11101 tcaccgcata gcccgcggcg cgcagaagct cggcgatccg gtctccttcc tcggcgctga
    11161 tgacgcgcag tacggtgttg ccaaaggcga tgcggcgctc aagcaggatg ccgacgacat
    11221 tgccggtgga aaatccggct gcataaaaga ccgtgaggat gggctgggcc gagacctcgg
    11281 ccaggacctt ggcgacgaca aggagccaga cgctgacctc aacaaagcca agcatgaagg
    11341 cgattttggt ccggccatgg acgatggcga tcgtccgcac cgtgccgagg gtgacgtcgg
    11401 tgacgcgggc gaggaaaacg atgaggccgg tgataagggt agtccactcc acgacgatca
    11461 ttcctttcca tagagggggc ccggggcccg gcacccgcag gcgccgggct ttccaggcgg
    11521 accagtccgg agcctatttc tgcggccagg gaatctcttg atagcgctct ccggtctgac
    11581 cggcgatggt ccggtaccag gcttcaggat agggcgggtg ctgcacgccg ccgtgttcat
    11641 cccgaagatt gccgtcgaga aatttccaga tcaggaattc ccccagtttt ttccagcgtg
    11701 cgacggtctc ctcgccggcg ctgaccgaaa agccgctcag gtaatctcgc gcctgctggg
    11761 gtgaggtctt gtatagggcc agcgcggcgc gatcgatctg cgggacctgg gcgatgaaac
    11821 gtccctcgag ctcgcgctgc acaatctgga cctccttgat catgtcgctg tagcgggaat
    11881 aggtataatt ggagacaaag ttgaaaaccc agaaagccga gttccaggag aaggcgtcga
    11941 acgagccggc gccgaccgca aagctggggg cgatgcggtt gatgccgcag tacatcggca
    12001 cataaacggt gcagtaggtg tcgtcgacgc cgaaccagag gatgcccccg atggggtcgg
    12061 gcagcgcggc gcgggactgg gcgacgaagg aaaagccggt ctgctgggtc gaggtggcgc
    12121 gctcatgaaa atatttgacg ctgtccacct tccattccat cggccgccag cgatagggga
    12181 ggccgtaggg tccggccccg acatcctgag tcatgtcgag ctcggtgccc tcgtaatggt
    12241 cgcgcatcag ctccatgacg tcgcgaacgc tcagcttttt gtccggcttg atccagaggg
    12301 gaaaaggggt gctgccgggg atgccgcggg catagtcgct gggcagtttg agcgaggggg
    12361 cggcgcgacg gaagccggcc caaacccgcg cctcacagaa gcgaagggcg ccgaaatcga
    12421 gcggggcgta ggtgtcggcg aagctgaact ccttgtccga acctttgtag taaccctttt
    12481 cccgcgcgaa ggagatgaca tccctggcgt agagagagtt ctgggggtcg ttgagcggga
    12541 actggcggat gcgcgcctgg ttggcgtggc cgcagatgta gccgtcgggg acgcggcgcg
    12601 ccacccagag ggcgccgggc tggccctcgc ccttgccgat catctccatg atccagacct
    12661 cgcgggggtc agagatcgaa aaggactcgc ccccgctgca gtagccgtat tcttcgacca
    12721 ggctggtcat gaccctgatc gcttcgcgtg cggtcttggc ccgctgcagg gcgagataca
    12781 tcaggctgcc gtaatcgagg ccgcccttgg gattatgcag ctcgctgcgt ccgccaaagg
    12841 tggtctcacc gatggcgagc tggtgttcgt tcatattgcc cacgaccgca taggtctcgg
    12901 ccacctgctt gatctggccg aggtagctgc cgctgtccca gtcgtagaca ttgatgaggg
    12961 ttccctcgat atgcctggcg gcggggagat agagcagttc gccgtacaat gtatgggaat
    13021 cggccgaata ggtgatcatg ctggagctgt ccgcggacgc cccgggggtg accataaaat
    13081 tggtgcaggc ctcggctgca ctggtcatga tgatcaggga gaggactaga gagaggagac
    13141 gcttcattga acctccggat gtgacggatg gattatgagt atgccgggcg gcggtgatgc
    13201 tgttccggct ggtctaaaag ccttcctgga tggtgtcgcc gatgatcttg atgccgcgtt
    13261 cgatctcctc ttccgaggac atggtatagt tgaggcgcat ggtctccaga ccatcgcctt
    13321 cgcaggggaa gaagaatttt ccgggcacga aagcagcctt gcgctctatg catttccagt
    13381 agagtttttc ggcatcggtg cctttgggcc cctctaccca gatgaacatg ccgccctccg
    13441 gtttgttata gtgccattcc ctggggaaat agcggtcgag cgcattgagc atgacctcct
    13501 gtttcggctt gtagaggtcg atgatcttgg gaatctgctt ctcgagatat cctccgagca
    13561 gatactcagc ggccatggcc tgattgaagg tactggtatt gaggtcgacc ccctgtttgg
    13621 cgagcaccag ccattttctc accggctctg gcgccacgca gatgccaatg cgcagacccg
    13681 gggcgaagac cttggagagg gtggttgtgt agacggtgcg ctcgggaatc agggagtaca
    13741 tggtcggcag ggcttcgccg cggtagcgca gatcgctgta gggatcatcc tcgcagagca
    13801 ggacgtcata tttgtcaaga agttcggcga tctgatggcg gcgggcaacg ccgagagagc
    13861 ggccggtcgg attctggaag gtcggcacga ggtagacgaa tttgactgca tgagcggcaa
    13921 ggatgcgttc gagggcgtcg gggaggatac cctcatcgtc catggcggcg cagacatagc
    13981 gtggtccata gggattgaag gcggagaggg cgcccaggta ggttgggcct tcaaccgcga
    14041 ccgcatcgcc gggggtgatg agcaccttgc cgagggcatc gagcagcccc tgggatccgc
    14101 tggtgatgca gatgccggcg gcggtggtct cgatcccttt ttgggccagg taggggacca
    14161 gggcctcgcg cagggggaga aagccctcgg tcatgtcgta ctgcagggcc ttggtgccgt
    14221 atttttccag cactgcggtg ctcaattgct tgatgacctc gaagggataa ctctgggggg
    14281 caggatatcc gccggccagg gagatcatcc cgggctggcc gacaaccttg agaatttcgc
    14341 ggatgatgtt ggcgcccata gtgctggtgc ggtcggcgaa aagggtttca tacttcatga
    14401 cagagtctcc atgttaggct gcatgggcca gagtatgtgg acggtacggt agagcgaaag
    14461 agaatggatg tggacaaggg ggatggaacc ggactggttt gagtgaaaag gcatgacagg
    14521 caccctttgg tttggttcct gaacatagcc aaaattatgg tgattatcaa gcaaaaacag
    14581 caaggcttcc ggaggccagc caatttggtt ctttgtttgc cgctccattt ttgttacatt
    14641 tagcctgaat ttgactccca tggaaggcga tgaagcataa aaggttgata tacggcgctg
    14701 ccgcattggg gatcctgctg gcgcttctgg tcgtaattcc accctctccc tcccggctgg
    14761 acagcagccg cctccattcc aggcgcatct atgacgaccg gggcaggctg ctgcgcgagg
    14821 tgctgtcgga tgaagaggga cggggcatgt ggatccccct ggccgagatg gggccggatg
    14881 tggcagcggc ggttgtcgcc atcgaggaca aacgctttta ccgccatccc ggcattgatc
    14941 tcctcgccct ggcgcgctca acctggctga atctgcgcgc cggggaagtg gtcgcaggcg
    15001 gctcgactct gacccagcag ctggcgcggc agctctatcg cctgccgcgg cggtggtacg
    15061 caaaaccggc tgaagccttg ctcgccctgc gcctggagtt atggttgagc aaggaggaga
    15121 tcctcgagca ctatctcaac cgggccccat tcggcaatca actctttggc gtggcgtcgg
    15181 caagccggat ctatttccag cagtccgccg tcgagctctc gctggcggag gccgcctttc
    15241 tcgccggttt gccgcagtcg ccgagcggtt ataatcccta taccggcttg gcgcgggcgc
    15301 gggagcgcca gctgcgggtt ctggcggcga tgcggaggca gggggtgatc ccggaggagc
    15361 gctaccgcgc cgcctgtgcg cagccgatcg aggtggcgct gcgcaagagc gttttcgcag
    15421 ccccccattt ttgtcagatg gtgctggaaa aggccgaaac agaccgcttc gagattcata
    15481 ccaccctcaa cagtgcgatg cagtcgcgga tagaaaagct ggttgagggc catctcgccc
    15541 agctcactgc ccatcatgtc accaacgcgg cggtgctggt tctggataac agcagcggcg
    15601 cggtcaaggc ctgggtgggg tcgcgcgatt tcttcgacgc cctttatcag gggcaggtgg
    15661 acggggtgcg cagcctgcgg cagcccggct cggcgatcaa gcccttcacc tatgccctgg
    15721 cccttgagaa cggctttacc gctgcctccc ttctggccga tatggagagt tatgcggggg
    15781 aagagggggg cggtaccctc gtccacaact atgacgaaaa gtaccacggt ccggtgcgca
    15841 tacgcacggc cctggcctgc tcctacaatg tcgccaccgt gcggctgctc gaagccctcg
    15901 gggtcgatct cctgctcgag cggctgcggc aggccggctt ttcctccctg caaaagccgg
    15961 cttcattcta tgggccgggc ttgaccctcg gcaatggtga ggtctccctg agcgaactga
    16021 ccaacgccta ccgcaccctg gctaacggcg gactggtgcg gccgtttcat tttctccggg
    16081 acgaagccgc cgcggcgggc atggcggacg gagagtcggc gagcggggag tggcagggtg
    16141 agcggatatt ctcccgcagc gccgccttta ttatcacgga tatcctcagc gacccccatg
    16201 cgcgggcgcc cgccttcggc ctcgggggtc cgctccatct gcccttcccc tgcgccgcca
    16261 agacagggac gaccaaggat ttccgcgaca actggacagt cggctatacc accgtctata
    16321 ccgtgggggt gtgggtgggc aacttcgacg gacagccgat ggaaaggatc agcgggatca
    16381 ccggggcggc gcccctcttc cgcgatgtta tgctcgaact gcacgctggc ttcgacccgg
    16441 cgccctttcc gcttccccct ggcgtggtgc agagggctat ctgcccggtt tccgggcagc
    16501 ggccgtcgac agcttgtccg gggagcgcgg atgaatggtt catcgccggc acagaacccc
    16561 gctccgaatg cagcgtgcac cggctggtgg cgctcgaccg ccgcagcggg cagccggcat
    16621 cgccggccac cccgcgcgcc gagatcaggg aaaccctcta tgaggtctgg ccgccggagt
    16681 accgggcctg gatggcggcc aatgatctgc cgatgcctcc ggccgccctc tccgtccccg
    16741 gcgaagcgga agcgccccgg ctggtgatca cctttccaaa ggagggcgac atcctgcgta
    16801 tcgatcccgt tctgcgccgg gagttccaga ccatcctgct cgaggccgtg gtgccggaag
    16861 ggatcagcga ggtcgaatgg atgattggcg acagcaccct ggcgcgtctg catgccccct
    16921 atcgtctgcg ctggcccttg acgcccggca gccatcgcct ggtcgtgcgc ggccgcggca
    16981 ggaacgggac agtgagcagt gacccggtca ccttccgggt gctgtgagcc ggtggaacaa
    17041 agccctgatg ggggatgtta cacttttata ttcttttttt tgatcgggat aaacgaaatg
    17101 cgggagacag gagtagtcct atccagcagc ggcagggtgg cgcaggtgcg catcctgcgt
    17161 tcgccgcagt gcgcggggtg ccacggctgc attgagctgg gggagccgca ggagcgcctc
    17221 atcgaagtcg agaatgacct ggacgcgcca gccggcgccg tggtcgagtg cgagatcgct
    17281 tcggggcagg tcatcggcca caccctgctg gtctttttct ttcccctctt cggcatggcg
    17341 gcaggctatt acgctgccgc cggccggctc agcggccggc cagccgccga gggaatcggc
    17401 gtcgccggcg cttttgccgg gctcctcctg gctttcctgc tggtccggct cgtcgacaaa
    17461 cactggggga gaaaacatcc cactaccggc cgcatttgcg gttatgccgg ggccgggacg
    17521 gaggaggaaa actccttttc ctgtccacag cactaaaact catggtatga ccagatagga
    17581 tcagcatgcg tcgatatctt atactcgtat taacagttat cctgccggcg gctcttgggg
    17641 ccgccgtgca gcagggcgtt cgactgctct ccggctcgaa cggccagctc gtactcgagt
    17701 tcgtccccga gcactggagc atcgattctg tgcgcgtcga cggacgcttt tatcagagtt
    17761 atctctttgc cggagcggag ctgaccggag cgcccggggc gccgcagctg cccgcccgga
    17821 cggtggtcgc cggcatcccg cccgcgggcg gggtttcgct gcgcatactc tccgccgagt
    17881 atgaaacgcg cacctcctcg gtactgccga tgggccgggt ggtccgcgac gggctcctga
    17941 ccaggaccga gtaccagcag gatcctgtcg agtacggccg tcaggatttc ggacccggaa
    18001 tcgtccgcat cagcgaacct gccgatttca gggggcagcg cgcggtgaat gtcacctttt
    18061 atcccgtgca ggccgcgccg ggcgcccgcc aggtccgcct ctaccgccgc atcgtcgcgg
    18121 agctcagctt cgcgtcccct gcgacgggaa gcagcgcttc tgctgcggtc ggcgccgctg
    18181 cggatggcct gctgcaaaat ctgctcatca atcccgaaca ggcgcgggca tggcggacaa
    18241 gcgagccggc gcggttggcc aagagccacc gcgccctctt cccgggcgac aactggtaca
    18301 agattatcat ccggggcgac ggcagcggcg gcaaggaggg cttgtacaag ctgacggccg
    18361 cggccttgag ccaggcgggt gtccccatcg cctcggtgga tccctccacc ctccagctct
    18421 tcaacaatgg cggccgcgag ctgccggaaa atatctccgc cgccagaccc gattcactcc
    18481 tcgagaatgc catcctcgtc aatggcgagt cggatggccg cctcgacagc ggggactata
    18541 ttctcttcta cgggcgtccc ctcgaggggg agtactattc cagttcggat ggcaagtacc
    18601 gccactatat caaccgcttt ggctatgaaa acgtctactg gctgaccttc ggccttaaca
    18661 agggcaagcg catgcagagc cgcacctcgc tgccagcttt cggtgaggcg gaaagcagct
    18721 tccgagatct ggcctacaac gaggatgaac gggccaatat ctacaagtcc ggctccgact
    18781 ggctcggctt cgagctggcc agggacaaga acaactactc ccagtctttc tccctgccgg
    18841 gcgctgtgcc gcaggcgtcg gcagttttcc gctaccaggt ggcggcgacg acctcgggct
    18901 cccatctctt caaaatgaat gccaatggca acagcctcgg tcagttcaac ctcgccgggc
    18961 agttgagcgc ctacactccc cgtcagaccg agttcaccgc cgccggggtt ctgctcgagg
    19021 ggagcaacac ggtgggaatc gagtatggcg cggcctcgga catcagtcag gcctacgtcg
    19081 actgggtgga ggtcgaatac cagcgccgct tccagcctgt caatgaccag ctcatcttca
    19141 attctcccct gcgcgagggg gccgcactat acgagatcgg gaattttgaa cggacggaga
    19201 tggcagtcta cgatatcacc gtttttaacg atgtaagtgt gatcgcaggg acgcagctgg
    19261 ttgggtcgaa gatccgcttc tccgatcaag tcaccactca gagcccgcgc cgctatctgg
    19321 ccctgacccc ggccgcctac atggcggtga cggagatccg ccgggatgca gcctccgatc
    19381 tgcgccggcc tcgcgacgtc gactatatca tcatcactca cgacaatttc taccagcagg
    19441 ccatgcagct cgagagcctg cgtgaagact ggaactccag cgaccgcctt gaaaccgagg
    19501 tggtcaggat ttcggacgtc tatgacgaat tcagctgggg tttgaccgat cccgtcgccg
    19561 tgcgcgattt tctcgcctat gcctatgcca actggggagc cccaggctac gttttgctct
    19621 tcggggacgg tcactacgac tttcgcaata tctaccgcta cgggactccc aatctggttc
    19681 ccccctatga aagcccggag acctccgaga tcgcctcgcg aaccagcgac gactggtaca
    19741 cctatttcaa ggggaacaac aacggcatgc agatggccat cggccggctg ccggtgcaga
    19801 cggtcgaaga ggcccagggg gtggtcacca aactgatcga gtatgagacc aaagccctgg
    19861 ccgaggagtg gcgcaagact gtgaccgtcg tcggagacga cgaactggtc tccggaggca
    19921 agggcgagga gctggagcac acacggcagg cggagtggct ggcggagcgc tatgtcccgg
    19981 cggcgcttaa tgttgaaaag atttacctca tggagtatcc ggcggttcgc accgccagca
    20041 tctccggggt gaccaagccg caggcgagcg ccgccttcat cgagcgggtc aaccagggca
    20101 gcctgatcgt caactttatc ggccatggcg ctgatgagtt gttgacccac gagaatatct
    20161 tcaatctttc gaccgacttt gataaaatcc agaatgcccg gcgctatgcc ctgtggatcg
    20221 catcgacctg cgagtttgcc tactgggatc agccgcagaa gcagagcttc gccgaatatc
    20281 tggtcaatgc cccggcaagg ggcgccatcg gcatgatctc atcgacgcgg ctggtctatt
    20341 cgagcgagaa cgccaccctg aattacaatt tcttcaacta tctcttcaac ggctatgagg
    20401 cctccggcag ggtcgcccgc gtcggggacg ccctgatgct ggccaagagg ggctcatcgg
    20461 ccagccagct caacaatgaa aagttcgtcc tcctcggcga tccggccatg cgtctggctg
    20521 ccccgcgcta tcgcgccagt gtcgataccc tctcacccgg cgccaccctg caggccctga
    20581 ctcactatac ggtcaagggc agcgtggcga gggcgggcgc ctcctggcag gatttcgacg
    20641 gcacactgct gctgcgggtc tttgacgccc gcaagccgcg cacctatacc agtgagggcg
    20701 gagcatcgat caactatatc cttccgggca ataccatctt ccgcggcacc gccctgagcg
    20761 gggccggccg cttcagcgcc cagttcatcg tgcccaagga tatctcttat ggcggcagcg
    20821 acggccgcat cagcctctat ttctggagtc cggagggaga tggcaccggc tatatgaaca
    20881 acctcacggt ggacggctcg gcgatggggc tggtcgaccg tgagggcccg ctggtcaagg
    20941 tccatttcgg caatccctcc ttcgcctcgg gggattacac cacccgcaat cccgtcctcc
    21001 acgtccagat cagcgactcc ctgagcgggg ttaatatcgc cggcgacatc ggccatcaga
    21061 tcatcatgaa tctggatgag ggggaaggca aaaacatcac cgaatatttt caatactaca
    21121 agggcagcta tacctcgggc gagctgcagt atccccttgt gcagctcacg cctgggcgcc
    21181 acagcctttc gctcaaggcc tgggacaact ccaacaactc cagcatcgcg gagatcgaat
    21241 tcatggtggt ggcggaatcg gagttgaccc tgagcaatct tctcaactat cccaatccca
    21301 tgagcgatcg cacccagttc acctttgagg tgagccggaa cgcggaggtc gccctccagc
    21361 tctacaccgt agccggccgg ctggtgcgca aattcccggc gatgcaggcc gaggtgggct
    21421 ttaatgtcta tccggaggta tggaacggga tggatgcgga gggggatccg gtggcgaacg
    21481 gggtctatct ctaccgggtg cacgcccgca gcgccggccc ggagggggcg gccgaaaccg
    21541 atgctgtggg caaggtgatc gtggcgcgtt agttccgggc tccggcggcg gccggatcag
    21601 ttctggtcga gaacgtcccg caccttgcgg gtgatgttct cgagggtgaa gggtttggcg
    21661 atgaaagcca ccgtctcccg gtcgacgttc tcatgggcca tgagcacatc gctggcataa
    21721 cccgacatgt agagcacttt taggccggga ttggttttgc gcagccgctc ggccagctcg
    21781 gctccgccca tctgcggcat gaccacatca gtcaggagca ggtcgattgg acccgaagcg
    21841 cgttcactgg cgatgagggc ctcgcccgcc agggccgctt cgatgacctt gtagccctgc
    21901 agctgcagca tgcgggaggt gagcacccgt acatcctctt catcctcgac cagtaaaatg
    21961 gtctctttcc cccggggcag ggtatcatag acggcgcgca ggtcagtctc cgcggcggct
    22021 tcggccacca gcggcaaaaa gatcttgaag agcgtcccct tgcccttctc gctgtaacac
    22081 tccacatgcc ccttgttctg gcgcacaatc ccgaagacag tcggcaggcc caggccggtg
    22141 ccctgctcct tggtggtgaa gaagggctcg aagatgcgcg ccttggtctc ctcgtccatg
    22201 ccgatgccgt tgtcctgcac cgagagcagc atgtactcgc ccggggtggc gtcgatgtgg
    22261 ctgcggcagt atttctggtc gaggctgacc cggtcggtct cgatgacgag cttgccgccc
    22321 ctgggcatgg catcgcgtgc gttgaccacg aggttgacga tgatctgctc gacctcagcc
    22381 ggatcggcct tgatattgcc cgtgccctcc ttgaacctcg ttaccagatc gatattctcg
    22441 ttgaccagcc gcttcagcac gctcaccatg tcgatgaggg tggcgttgag atcgatcacc
    22501 ttctgctcca tcggggtgcg acggctgaag gcgagtagct gtttggtgtg ggtggaggca
    22561 cgcgcgcagg ccttgatgat ctgctgcaca tggccgtaga ggggatcgct gggcatgatc
    22621 gacttgatca tcagctcgga gtagccgttg atgatggtga gcaggttgtt gaaatcgtgg
    22681 gccacgccgc cggcaaggcg gccgatggca tccatctttt cggcgtgata gacctgcttc
    22741 tgcagatcga tttcgtgggt gacgtcgcgc ttgacggcga cgtagctgat gatctgtccc
    22801 cccttctcct tgatgggcga gatcgtcgcc tcttcctcga agagggtgcc atccttgcgt
    22861 ttgttgacaa agtggccgtg ccagctcttg ccgctcatca gggtggtcca gatttcccgg
    22921 taaaaggcgg cgtcatgttt gccgctgtga agaatgctcg gttttttgcc gagaacctcc
    22981 tcccggtcgt agccggtgac ccgctcaaag gcggggttga cgtactgaat ccggttttcc
    23041 acgtcggtga tcatgatgct ctcggccgcc tgttcgatgg cgatgaccag acgctggtat
    23101 tcctcctcgg agcgcttgcg gtcgctgatg tcgtgcgcga agcagaggac gaaatcctga
    23161 ctgtcgagct ggtaatcatt gagggtgagt tcggcaggga cgatctggcc ggatttggtg
    23221 ctgaggcggc tttcaaaggt ggcggaatgg cggctggtca tgctcttgag gagagccttc
    23281 catttctcag gcaccaggtt gggatcgacg gccgaaatcg agagtgtgct cagttcgggc
    23341 atggtgtagc cgagcatctt cagacccgcc tcattggcga gggcgaagcg cccctcgtgg
    23401 ttgagccaga agatggcaac cgcggcgtgg tcgacggcga acttggtgaa gcgcagggcc
    23461 ttttcgatgc gccgggtgcg ccgctcgtgc tctttttttt cgaccgcctc gcgggcggag
    23521 gcggccaggc gaaagatatg gtcggaatga atgacatcgg cggccccggc cttcatgcag
    23581 gcgacagcga tttcgctgtc gaggctgccg gagaggacaa tcagggggat atcgagatga
    23641 tgctcctgca ggtgggcgag ggcctgcagg gcatcaaaag cggggagaaa ataattggcg
    23701 atgatgagat cgggtttgcc cgccagcgcc tgggtgaaac ccttggctga attgacacgg
    23761 acccactcac cgagcaggcc ggcattcttg aattcgcgcg caatgatctc ggcctgctgg
    23821 gcctcttcct cgatgatgac aagccggaaa gcgctctcca ttgctgaacc catctctcct
    23881 cgctatggtc agcggatcat ttccgaggtg tcggcggcct cggcctggag ctgcgtctca
    23941 tcctcttcaa gaaaaaggat atagatgagc agcatgacca tgccgacgct gagggcaatg
    24001 tccgccacat tgaaaaccgg ccagcgctcc agactgtagc ccgcaaaggg gatggagaag
    24061 agcttgaagg ccggaatgtg aatatcaaag aattcgaaat cgagaaaatc gaccacccgt
    24121 ccgcggagga tgcggtcggt cagattgccg agggcgcccc cgagtataac ggtcatcgca
    24181 agccgcgccc atcgctgccg ctctttcagt ttgaagagat agagcatgat gacgatgctg
    24241 gcgaccactg cgaagacagt gaaaaagagg ttgttgctga tgcggattcc gaaagccatg
    24301 ccgggatttt cgacataggt cagacggaag aaatccccga gtaccggtat cgattcatgc
    24361 agacgcatat tcgacgcgac gacccatttg gtcgcctggt cgagaacgaa aaggaagagg
    24421 gagacccata atatgcggta ggatttcatg gctatctttt tgacatctcc tctttggact
    24481 tgcattcgat gcagagccgg gcgtggggtg cggcctccag gcgttcaaag ctgatctcct
    24541 tgccgcattg gacgcagtag ccgaactcgc ccttttcaat gcgctcaagc gccaggttaa
    24601 ggtgatagat caggttgccg tcgcgggcag caaagaagta ctgctgctca cggtccatgg
    24661 tatcggtgcc ctgatcggcc atatggaaag agtaggagga gtgatctcct gaagtctcct
    24721 tcatcgaggt gttgaggccg ctcgatttca tatgatcaag ctccatgatc atccggtcgc
    24781 gtttttccat gatgagcttc ttgaggtagg ccagttgttc tttggtcatt ttcgttctcc
    24841 caggcaaaaa atggatcgta ttacaaacgg gaaatacccg ctttcaggag ctggtcttcg
    24901 atcttgagct ccttttgaaa agtgccgcct gtgtcaccca gcctcaggga ttcgcacagg
    24961 gtctcactgg tgatatattc gctctggctg gcaaccgcct gctcgagctc cggggaggcc
    25021 tgcagccaga tgttgatgcg gtcggtgacc tcgaagccgg cctccttgcg catgttctgc
    25081 acccggttga cgaactcacg cgccagcccc tcacagcgca gggcttcggt gagattcagg
    25141 tccagggcga cggtcaggtc aacttcgctg gcggcgacca gtccttctct gttttcggtc
    25201 tggatggtga tatcgtcggt ggtgatgcgg gcttcgtggc cctcgattac catctgcttg
    25261 ctgccgctgg cgaggaggga atcaatatcg gcctcgctga aggcgcgaat cgcctcggcc
    25321 gccttgttga catttttgcc gaatttcggg ccgagatttt taaagatggg ctcggcgcgt
    25381 cggctgagca gggccttctg gtcgctgacg aactgcaggg acttgacatt gagttcgtcg
    25441 aggatgaggc cggacatgcc ctcgatcatg cggtgatgtt tttcgtcgga cgccatgacc
    25501 agaatcgcgg ccaggggctg gcgcaccctg gtgccggcct cattgcgcag cgagcggccg
    25561 agaaagacga tgcggcggac caggtccatc cgcacctcga gcttttcgtc gcgaaaatcg
    25621 gccggggctt cacctgcccg gggatagctg gccagatgga cactgtcggc atgccccgcc
    25681 ccgccgtcgt tgagcttgcg ataaatctca tcggcgatga agggcgccat cggggccatc
    25741 agccggctga ccgtgaacag agcctcataa agggtttgaa aggccgccag tttgtcctcg
    25801 cccatctccg ctttccagaa gcggcggcgg gaacgacgca cataccagtt cgagagatcg
    25861 tcgatgacga aatcggagat ggcccgcgaa gccctggtga ggtcgtagcg ggcgagatgg
    25921 tcattgacgc gtttggtcag cgccgtcagg gacgaacaga gccagcggtc gagttcagaa
    25981 cgctcggcga ccggaatgcg cggcgtggcc gcataggaga actggtcgat gttggcgtag
    26041 agcgcgaaaa aggaataggt gttggccaaa gtgcccagaa acttgcggat gacatcgcgc
    26101 acgccctcgg gatcaaagcg ggtgggcagc cagggcggac tcaccgtgag cagataccag
    26161 cgcagggcat ccgcgccctg gttgtcgaga aactcgatcg gggccacggc gttgccgcgc
    26221 gtcttggaca tcttctgccc ttccttgtcg aggatcaact cgatcgagac gcagcttttg
    26281 taggccggct gatcgaaaag gaaagccccg atgaccagca gggagtagaa ccagccgcgg
    26341 gtctggtcga ccccctcgga gatgaaatcg gccggaaagc ttttggcgaa cttgtccttg
    26401 ttctcaaagg gatagtgcca ctgggcatag ggcatactgc cggaatcgaa ccagcagtcg
    26461 atcacctcgg ggacgcgctc catcacgccg ccacaggcgg ggcacttgag tgggatgcgg
    26521 tcgaccatgg gtttgtgcag ctcgagatcc gcggggacct cgtgggcggc cttttcgcgg
    26581 agttcggcga tgctgccgat gcactcctgg gcgtcgcagg tggtgcagcg ccagaccggc
    26641 aggggagtgc cccagaagcg gtcgcgcgaa agcgaccagt cgatgttgtt ctcgagccac
    26701 tcgccgaagc ggccttcacc tacctctttc gggtaccagt cgattttctt gttgttggcg
    26761 atcagccgtt ccttgatcgc ggtggtctgg atataccagg atttgcgcgc gtaatagagc
    26821 aggggcgatt tgcagcgcca gcagtgcgga tagctgtggg tgatcggctc ggagcggtag
    26881 aggacgccgc gctctttcag ctcgcgaatg atccctttgt ccgcctcctt gacgaagagg
    26941 ccggcccagg gcgtgacctc ggcggtgaat ttgccgctct tgtcaacggg ctggaggacc
    27001 ggcagatcgt atttcttgcc gagctggtag tcgtcctcgc cgaaggcagg ggcgatatgg
    27061 acgatgccgg tgccatcgcc ggtggtgacg aaatctccga gcacgacata gtggctgcgc
    27121 ttgtcgggtt tgacaaaatc atagatcggt tcatattcga ggccggccag tttttcgccg
    27181 ctgaattcgc cgacgatctc gtaatcgccc tcgagcacct cgaggcgcgc cttggcgagg
    27241 atcagaaact gatcctgatg cttcaccttg acataatcga tctcgggatg gagggccagg
    27301 gccacattcg agatcagggt ccatggcgtg gtcgtccaga ccaggaaata ggtttctttt
    27361 tggcccttca ctctggcgcg gacatagacg gaggggtcgc gcacctcttc atagccctgt
    27421 gatacctcat ggctggagag gggggtctcg cagtgggggc agtaggggag gatcttatgg
    27481 ccctgataga tgagaccctt cttccagaac tccttgagga tccaccacac tgattcaata
    27541 tagtcgttgg tataggtgat gtagggattc ttgagatcga cccagaagcc gatgcggcgg
    27601 gtgatttcat cccactcctg cagatacgcc cagacgctct cccggcattt ggcgttgaat
    27661 ttgtcgatgc cgtattcgat gacctggtcc ttgccgtcca ggccgaggct cttttctacc
    27721 tcaatctcca ccggcaggcc gtgggtatcc cagccggcct tgcgttcaac ccggaagccc
    27781 tgcatggtgc gcaggcggca gaccgcatcc ttgacggtgc gggagatgat atgatggatg
    27841 ccgggccggc cgttggcggt cggggggcct tcgaaaaaga caaagggatt gttttcgtcg
    27901 cgctccgaga cgctgcgctc aaaaatcctc tgttcctgcc agaattcaag gactctcttt
    27961 tcgatctccg ggaaattgag tttttcaggt aaaggctcgt acatgggagg tggttccgtt
    28021 ctttctgcgt tttgggatgc ttctataacg atacttcatt tagagatatt tcttcaggat
    28081 cggacccagc cgggtgatgc tctcctgtgg ccacgccccg cgctcggtga tgatcgctcc
    28141 cgccagcgcc tcctggcagg cacaggcggg ctgccagggg aaggaggcga tgctgcggcg
    28201 gatcagctct ttggcgagag cgctgctctg cctgaggttt tccaccacca gctccaccga
    28261 tacagtctgg ccggtctccg cctcccacca gcagtcgtag tcggtgacca tggccagggt
    28321 ggcgtagcag atttccgctt cacgggccag ccgcgcctcg ttcatctggg tcatgccgac
    28381 cacatccatg ccccaggagc ggtagagctg tgattcggcc agggttgaaa aggccggccc
    28441 ttccatattg acataggtcc cgccccaatg gatcgaagct cccatctccc tggcggcagt
    28501 gtagagaatc tgtcccacgc tttcacagag gggatgggcg aaggtgatgt gggcggcgat
    28561 gccaccgccg aaaaaggtgt tgggccgggc ctgattggtg cggtcgacga actggtcgac
    28621 gatcaccaga tcctggggct tgaggccggg cttgagcgat cccaccgcgg agaccgagat
    28681 gatccaggcg gtatccaggg atttcagggc ccagatgttg gcgcggttgt tgacctcggc
    28741 cggcagaatg cggtgtccgc ggccgtggcg cggcagaaag agcacctccc gcccctcgag
    28801 gcggccgagg atgagcaggt cggagggatc gccgaaaggg gtttcgacgc gcacctcgcg
    28861 gatctcctcc agaccttcga tctggtagag ccccgaaccg ccgatgatgc cgatgcgcgg
    28921 catgcactgc tccttgctag atggcgcgga tgatggcgcg cagcagggcg cggaagcgcg
    28981 gccccagctc cgcggcgaca tgattgaccg cttcatggct gacgctgacc atggctccgg
    29041 gcagggactg gtcggtcacc gccgtgaggg ccagaacctc catgcccagc tggcgggcga
    29101 ccagggtttc cggaaccacc gacatgccga cggtatcgac gccgaggatc tgcagcatgc
    29161 ggatctcggc ctcggtctcg aaggcggggc cgatcaccgc cccgaagatg gtgtcatgta
    29221 cggtcacgcc ctgttcggcg gcgatgcgcc gggcgaggcc gcgcagaacg gcgctgtagg
    29281 gctcgctcat gtccggaaat cgcaccccca gctcggggtc atagggtccg atgagaggat
    29341 tggtccccat cagattgatg tggtccctga tcagaccgat gctgcccggg gtcaattcag
    29401 gacggatgcc gcctgcggca ttggtgagaa tgagccgctg gatgccgagg gcgcgcataa
    29461 cgcggatggg cagggtgatc tgccgcatcg ggtagccttc gtagccgtga aaacgtccct
    29521 gcatggcgag cagggtttta ccctcaagtt cgccgaggac gagccggccg gcgtggaatt
    29581 tgaccgtcga tgcaggaaaa tgggggattt cgtcataggg tatgacgcgg gcctccccga
    29641 tctcgtcggc aagcgcgccc aatcccgtcc ccaggacgat ggcgagatcg aaggcccgtg
    29701 catcccgggt gcggatccag gccgccgcct cctgtacctg ttctttcaat tccactgcgc
    29761 cgtactcctg aatgcccgcc gcctcattaa atgacaatct gatcggagag gttgttctcc
    29821 ctgccctgtt cctcctcccc gttcttctcg gggagttctt gttcgctttc gtcgctgaac
    29881 tgcatgcccg tgggctcgtc aaagcggcgc tcgccgcgga taatgcgcgg gccttcgctg
    29941 cgggcgaaga aggagccggt ttccgcttca gctggaccgg aggacccggc gggcgggatg
    30001 ttgaaggagg aagagggcgc cgccggtgca gccggcgccg cgccagccgg tgcaatgccg
    30061 gaagctgttg gggcggcacc atgggtctgc gcctgcgccg aaacgggcgc atggggctgt
    30121 tcagatccag gggccggggg attttccttt tcggccggca ggaccgggcg cggcagccgt
    30181 ccgcgcaggg cggggccctg gggctcctcc gtattactgc tctgcttgat catggcgtca
    30241 tcgatgccga gcacgtcgag cagttcgatc tgggattcga gcagatggcg caaacggcgg
    30301 gagaaggatt ccttctgggc gcggagaaga ttgatctccg agcgcagctc ctgcagctcc
    30361 tccttggcat tttctatgat cctttcggcc tgcagttccg cctcatggat acgcagctcg
    30421 gcttcgcgca gggagttgaa gcgcgattcc tccaccgtgc tggtggcttt catcagggtt
    30481 tcgcgcaggg tgtgctcgac ctgctggtag tcgcggagct gggtgcgcag tttgatcagt
    30541 tcgtcgctct gctggttttt ctcgcggagg acgatttcga gttcgtcggc gaccatgatc
    30601 agaaaggctt ccacctcttc cggatcaaaa ccgcgcatgc ttttcttgaa ctcctgcttg
    30661 cggatgtcca acggggtcag tttcacagtg gtctccgagg tttaatttct ctctccaaac
    30721 agtgcccggc cgaggcggat gatggtggcg ccttcttcga tggcgatttc aaaatcatcg
    30781 ctcatgccca tggaaagctg gctgatgccc aatccggatg cgttgagacg ttcctgaacc
    30841 ttgcgcagct ggctgaaaca ggaacggatg cgttgggagt cggtagaaag cgcaccgatg
    30901 gtcatcaggc cggtcaggtg caggtggggc aggccggcga gatcgccggc cagagcctca
    30961 gcctcgcccg gtgcgatgcc gaatttctgg ctttctccgg aagtattgac ctccaggaaa
    31021 cactcgataa ttttaccctg ggctgcagct tcgcgctcga tctcgcgggc gaggtgcagg
    31081 gagtccaccg attcgatgag cgtggcgaac tgcagcgcac gttttacctt gttgctctgg
    31141 agatgaccga tcatgtgcca ggagagcgga aggtccgcaa gcgattggaa ttttgtccag
    31201 gcttcctgga cgcggttttc cccgatgagg gtgaggccca gagcagccgc ctcacgcatg
    31261 cgctcggctg ggaccgattt ggtcacggcg agcagagcga cctcaccggc ctgccggccg
    31321 ctgtgcaggg cggccgcctg gatccgttcg gtcacatatt tcagattgtc aaaaatggcc
    31381 atccggtcat aatttttata acgttaaata tagatttatt ttgcatcaaa tgcaataaaa
    31441 atcgcggaac ctccgcagtg tcattccgct aaagtccttg ctaaataggg atggattttt
    31501 taagttaagt ttctgcgggg aggataccaa tgaaaaaaag atattgtttt ctgttctggt
    31561 ctgtgattct cctgattccg ctgacgacgc agcccctact ggccggccgc gtggtcacag
    31621 tcggccggga aggtccgccg caatatgatt acagtgcgct gcagcaggtg cattcctggc
    31681 tgacccaatt gcccgggggc gtgtcggagc cgctgacgat cagactttat gacagcgagt
    31741 ttcccgagaa gaccctggtc tgggaggtga gcgggacaga gaaggccccc attctcatcc
    31801 agggtgtggg tgatgtctgg ttcagcggca cgcgggtgaa gggggaggcc tacggccggc
    31861 cggtcctgat cctctcctgg gtcagccaca tcaccctgga aaacatcggc tttcgcgatt
    31921 ttgatgggcg ccaggagagc cgtgtcggcc tcaagatcac cggcaagggg aaaaaccgca
    31981 tccagagctg ccgcttcagc catttcttcg gcagccggag cgaagcggcg atctccctct
    32041 acaacgaggc ttcagggaat ctcatcgagc agtgcgagtt tgattccctc ggtgatgatc
    32101 tttacatgca tgccatttat ctgaacaaga actgccgcca caacatcatc cgcaacaata
    32161 ccatcaccta ttgcagcggt gaggctttta tggtgcgcaa cggcagcgac cggaactggt
    32221 ttgatggcaa tacggtcgtg cgctgcgggg gcgtcgccta ttacggtgag tggtacgatc
    32281 agagccccgg ctcgaatgag aaaccctcgc gcggcaaccg gatcacccgg gcgagcggtc
    32341 agggccctgc ggccaaatcc tcgggctata tgcgctccgg gtatatccgc aagatccggg
    32401 cgttgaactg gtcccgctac aaatggccca attacaagga ggactggacc gacagcaccc
    32461 tggcctatta catgccggat gcggacaaga accggcttgt cacggtggct gacgatatgg
    32521 aaatccgggt caaggcggag attgacgcca cgccggccta tctgcgcctg ggcaagagtc
    32581 cgatctacca tgcccaggtg gtgcccgagg gtgagctgga gggggcgcgg gtggagctct
    32641 ctttcgccga caccaccaac cggtggggag gagacgagcg ttatttgcgc gagatccgcc
    32701 tccacgacac ctccagccgg ccgcggcgtt ttacgacccc gctgcagctg cgcaccctcg
    32761 agggcgatct gatccatgaa ctctatcccg tgcgcgagct gcggggcaag accgtggaac
    32821 cggcagtgat cgccgtcgag ggggcgggac aaaagatcac tttcaccctg accaggatgc
    32881 agggggatta cgacctcttt atcctgccgc agaagggctc cctgagcgtg gaaaagctcc
    32941 gtgctcactt tgacgacgag gacggtgggg gcgatttttt tgccgaaaat gccctctcta
    33001 gcggatttct ctcgcttggc aaggcggcca gtcgggggca gggttcaagg gatgaaaagg
    33061 tgagcctcac cctggcggcg gggcgctcct tctggatggt gatcagctgc aagcccggcg
    33121 cgaaaagcgg ctgcacctgg gcggcccggc cgtgacggcg gggtgcggat gtacttgggc
    33181 cgccatggct atcggcaagc cgtggctaaa aaaaacattt ttattgtctt tataatattg
    33241 taaatttata gattagatta tcaatcgtcg tgtaatcacc tgcaagcata ggatagggca
    33301 tgccacaaaa aga
//
LOCUS       DAOPWU010000004        30068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_1464_length_30068_cov_5.570953, whole genome shotgun sequence.
ACCESSION   DAOPWU010000004 DAOPWU010000000
VERSION     DAOPWU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1464_length_30068_cov_5.570953"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKM23_00640"
     CDS             complement(<1..658)
                     /locus_tag="PKM23_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY90004.1"
                     /translation="MLIKLEKIEKIYEIGTVQVPALRGINLNVEKNEYIAIMGPSGSG
                     KSTLMNIIGCLDTPSSGDYLLNQHNVSTMDDDQLAEIRNREIGFVFQTFNLLPRASAL
                     HNVELPMIYNGTPAAGRRKMAEEALEKVGLADRMHHKPNELSGGQRQRVAIARALVNR
                     PSIILADEPTGNLDSRTGDEIMEIFEGLHEQGNTIILVTHEEYIAEHSNRIIRLRDGM
                     V"
     gene            complement(669..1913)
                     /locus_tag="PKM23_00645"
     CDS             complement(669..1913)
                     /locus_tag="PKM23_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNY90005.1"
                     /translation="MSNKMKIIIAVAAVTIIAAMVLVNLRKSRGEVIEITSAEVKRGD
                     ISRTVSGSGKIQPVTDIKIAARISAEIKKIHVKEGDAVKQGQLLVELDRESLAAYVEQ
                     IQSQLTSAKAGVKKADADYARIKDLFGKNLASQAELDAAEATRQSAASSLDQTRAALK
                     QAMDDLGKTRLTSPIDGTVTKLNKEEGEIAVGSQFQADIIMNLANLKDMEVLSEIDEN
                     EVVLLHLGDKTTIEVDAIRDTTFQGKVTEIAHVATTTGAGTQEQVTNFDVKIGITSNV
                     DLLRPGMSATVDILTETRPQVLNVPIQAVTARDLKADSLRNVGGKRKSGNPPTESGEK
                     KEDEKDEMTEVVFVVNSGTVKAVPVKTGISDDNNIEIISGLSEGQKIVTGSYKAISRL
                     LKDGSQVKEKKAAGKETDDKNK"
     gene            complement(1947..3323)
                     /locus_tag="PKM23_00650"
     CDS             complement(1947..3323)
                     /locus_tag="PKM23_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNY90006.1"
                     /translation="MNKFTSLILLILLGLGSAFAQEPAPEPLTLQQCIAIALENNSTL
                     RVAHKSMEIAGTNVTTATAAWLPQINSSFNTGRYIQGARVDIADVPTGIDPVTGRTIY
                     TQQEIYQEQTERNNHSARISLNQNIFDFGQTLYSIKSAKANKQASAQTMENTRQMVIY
                     NVKDAYYNLLKAIKLEQVYQEAVRLAEEEVQRAQTMMEIGISSQSEVFQARVTLGSSR
                     STLINQQNTVDAARADLNNALGRNPDIPVSVREDESQPIFPAITFADAANEAIKNNAA
                     LKAYEYQSRASLFNLKMAQMRYAPSIGGSISYGRSNEDLSRVFSSNLDQDFSATLGVG
                     IDLNIFNGLSDKAGVQRAKLNYQIDEENLAEQKRLLTAQVKQYFLQLEAYKDFIEINQ
                     QNIEAARENLRLQQEKRRVGSGTELEVTQAQVELTRAQANLVNAEYEAKIARARLETA
                     MGAAEAGL"
     gene            complement(3359..4087)
                     /locus_tag="PKM23_00655"
     CDS             complement(3359..4087)
                     /locus_tag="PKM23_00655"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNY90007.1"
                     /translation="MQEYSTSGVPSASEGNMGLLQRIINVFAAPSKSFAAVRENPRWI
                     APTVLVLLLSLIMTIYIAPAIQKEQHEKMVTQMENRGLDQEQIDSAIERINSPVMKYV
                     MYGSALIGGLAVILVMGGVWLFVARTLLGGTTTYKHMLEVVSLSMLIPSVGMLLKAPI
                     MRFKETMNVHFSLATFMPDSARESFIYKFLMNTDLFNIWYIGVLCIGIAVVAGLKVKK
                     VWPLVVGLLVLWYLGSAAMGGLFS"
     gene            complement(4158..5570)
                     /locus_tag="PKM23_00660"
     CDS             complement(4158..5570)
                     /locus_tag="PKM23_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNY90008.1"
                     /translation="MKKNNRLFDTALIKIIGEVAERSAIPAYAVGGYVRDLLLGKQGK
                     DIDIVVVGEGPAFARLLADRLRVKNVTVYKKFGTAMIRHRSTVIEVVGARKESYRGES
                     RNPEVSSADLLTDLARRDFTINAIAVSLNPADRGELIDPYDGRADLKAGIIRTPLEPR
                     RTFSDDPLRILRAIRFAAQLQFDIEPVTLEAIAAERERLRIITQERISEELMKILAST
                     KPSLGFHLMNDTGVLDLILPEISALKGVDQVAGYRHKDVFEHTLKVVDNVARVSDHPG
                     LRLAALYHDVAKPQTKEFKPGVGWSFHGHEELGARMLPAIGRRLRLSQEMIDYAQKLI
                     RLHLRPIHLAEEGVTDSAIRRLIFLGGAELDDLITLCRADITSGNPKRVASHLANFDL
                     VVERMQEVEERDRLRAFQPPVRGEEIMATLDLQPGPAVGKIKKAIEEAILEGVIPNDH
                     DAAFTYMMERKDELIAGPGE"
     gene            complement(5866..6120)
                     /locus_tag="PKM23_00665"
     CDS             complement(5866..6120)
                     /locus_tag="PKM23_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90009.1"
                     /translation="MGDDFIFASIWFFFDDGPVPHPVLQPESRAPQYCQHNMDCRLDR
                     KFNAMFDIVRIFHRQLRFPSQWLSKRSMLLLLFNDLTWPP"
     gene            6119..10630
                     /locus_tag="PKM23_00670"
     CDS             6119..10630
                     /locus_tag="PKM23_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90010.1"
                     /translation="MLRILFVLALMLGAAGDLSAQILRRETADPLQTGTASWIKVEFS
                     IAGGSRTYLNDNFTGTEFRIDLNVDGDDNPSTNLGDLYTLRGPAEIYTTPPGVPGGAK
                     DAVIYLPIRQGSSTPFPYDTDLRPWIWLVEGGGLQISGQSVSGPWGVIQYPSVYIECD
                     DGISPTIRHAYYYDDGSGHSPAVSGSHVGNQTPFDGYIDRVDLEWSEPMSSSNVTVND
                     QIFGGLGTTIHSMESIGSWNMIGPSWKRFTLWVRSSAPNSGISPILTYTQPLSLTDRF
                     REASTGQYQAYAASHARALTDKAGPAIISAHTKRAMRRQLLAAALADRRIEVTFSEPL
                     DFASVQGVDFTVNTTTASPNPITAIISPTSTSTSPSAVWEFQLTTPYATGDEVGAIQY
                     VLDRQVTDVVANYNGESLAPAPPTAPSNGPGLIIPITDGIFPIITHVLTHDAILPGDL
                     TTGGTNGWGYLDYVDVLFDHAMNTARVSTAGLQVTGEGIVTIAGTGTWIAPNTLRVIL
                     TATTPRVANTGLIPRVTYTNPGDPNGLNEPVNLGLAEALYSSDITLSPTNGQVVQIVD
                     NAGPAIIRALTAGTKRIRLVFSEKVNTSSWPINPTPGLPSPLFKWFVGPSNYDVSGIQ
                     VYFTGMTPSRRDSIVYLNHTGLAWTKNDSGAINYRAQGVVWDLASSSNGNLQYDDDVS
                     LSGTTRRLLGSDVKVERDNIPPILLRLETVDIDADGKLDHYRFSFDDLSPIYPKRSFV
                     PGNWTITGYDGLKSNLGVNLNVYNPGYAYYQPNAINAYGDTVAAYVAFNETTAGGPAL
                     TPYGGDTGDVPDVVVLANNGFADWADNPMAPLPVGITLERDDAGPAIMSAKTISRFDV
                     EAFMSEDLADWSVFSSDFFLNMAPNANYFGAWPIKEAKEVIPGKVLINTFFYSSSLGW
                     DPKSEGILRFDSQGVVEDVVNPVTNGNIQTNWVTVTSNAACTFVIQPVVSGAQVRGVP
                     FDVQVIARDKYGNVDRNFAGYLTFSTNLQQNEIVLPSGPKKLIDGIGTFRFTSWVNTT
                     DLVITASIATDVYPLDAASSGPVTVIDPIIDQPDYLTVEDVPGDQGGFVTLKWPFSAN
                     HQGMGATPVINYYEIFYTIKGSDTLHYWPQQIAAYNPSGTGSTMMTVDFPVVTSDSMA
                     FYVRAVWVPPTAAVKGGATLSIMAAKAGDLPLVIHTLGEESPVTSVLGATGASVVSGT
                     AMAKGRAVDNIAPMAPERMVAAREGAAVKLHWGPVTKGVNGTLERAGAIAYKVYMHET
                     NPYFNPDAEGTLLATTADTSLMVNSESLRQYYVVRAVDADNHSGISRRVGKYGFSLIT
                     AAKPRYNYISLPLETEISDARELAEAIGAGVKVVLRIDPVTNGYSTYYLPDLNYPATP
                     FALHEGMPVLIQADESAPASWFYCGAVPEAGEFQFALNREHKFSYNEIIVPLNRSDIT
                     NAAELAAEIGGVEVLLKIGADGRGFTNYWLPSLNFGNPMTPFSVAPGEPVLIQVNKTA
                     PALWPTYTR"
     gene            10646..11602
                     /locus_tag="PKM23_00675"
     CDS             10646..11602
                     /locus_tag="PKM23_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90011.1"
                     /translation="MKRKFTYILFGLLLLGLCGAALPQEPHIIGGKFLNPDGSVPAGG
                     EIRFQAYLQKSPGDTSAASWCSTGGGWSVEVKQTPMISYANWEAGDTLVVVFENVLET
                     SPYYGARKVVKHATTEDSPEFVGSISLPVELTTFTAAVKNTSISDVVMLEWRTVGESN
                     NLGFEVERSLDGKNFSKLGFVAGAGSTNLAQGYDYRDDRVEVGTYFYRLKQIDRNGSF
                     TYSDIREVEVTAPQRYELTQNFPNPFNPQTDIVFRVKEEGVVTLKVFDLLGREVKTLV
                     NEKKGAGVHRITFDAHALPSGVYLYSITMGSFHDVKKMLLVK"
     gene            11896..13680
                     /locus_tag="PKM23_00680"
     CDS             11896..13680
                     /locus_tag="PKM23_00680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNY90012.1"
                     /translation="MRNSMKTILLILVFAFVLTIVVDWGMGGFKSNAPQGVIAKVDGQ
                     SIHYEEFSSRLQNELAAYREQSGSDPEGYQLQQLETRVFDNLVQQRLLNEEVKAVKLN
                     ASDQEIIDEIFNNPPLELRQNEAFQDSTGAFDMQRYQAALNNPGAGEFWASVEEYLRT
                     TLPMRKLDNLLRATAVVSDQEVRAEYMKRQLKAQAEYILVPANDFLATAANPTDAEIK
                     AYHKQHEEEFKEAEKRIIEYVLLNFVATKADSQAVDEQAAELLAEARGGADFAELARL
                     NSQDPGSAPQGGDLGFFARGAMVKPFEEAAFSGKPGDIIGPVASQFGIHIIKVGERKQ
                     EEGEEKVQASHILLKYEMSPRTREALQEEAAYIAEAAKEQDLKSLATAENIKLERTEP
                     FAAGGFIPGIGMEPRLSGFVFRSKEGAVSDVIYAERGYIVVRVADIIPEHIKSWSDAR
                     ETITAKLKNEKAMELARQKCQTLYEQAKSTGSLAGAAAQIGAQVQQAADFTFSSYVAS
                     LGREARFVGAAFGLQPGAISQPVEGTRGYYLIKLLSKNSFNEQEFEAQKESLRAQLMQ
                     RKQQSIFGQWYTGLKEKADIKDFRKDYL"
     gene            14006..14422
                     /locus_tag="PKM23_00685"
     CDS             14006..14422
                     /locus_tag="PKM23_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNY90013.1"
                     /translation="MFKLSKKTEYSIMALRHLGRNESMTATVREIAADCRIPAPLLAK
                     LLQQLARSGLVHSLQGVNGGYRLKSAMAGISLAEVIEAVEGPFRVTACGGEASRCSRS
                     GFCDLRSAIAPVQGQMLSYLQNVTLADLHGSRGEED"
     gene            14423..15868
                     /gene="sufB"
                     /locus_tag="PKM23_00690"
     CDS             14423..15868
                     /gene="sufB"
                     /locus_tag="PKM23_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010958177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNY90014.1"
                     /translation="MSTEMNQLESLANQEYKYGFVSDIDAEALPRGLNEEIIRTISAK
                     KREPAFMLEWRLKAFRHWQTMTEPRWANVKFPPIDYQDIIYYSAPKSHSGPKSPEEID
                     PEILALYQKLGIPLEEQQALAGVAVDAVLDSVSVATTFKEKLSQLGIIFCSFSEAVQN
                     HPDLVQKYLGMVVPSTDNFFAALNSAVFSDGSFIYVPKGVRCPMELSTYFRINAANTG
                     QFERTLIIAEEGSYVSYLEGCTAPIRKENQLHAAVVELIALDNAEIKYSTVQNWYPGD
                     KEGRGGVYNFVTKRGLCAGVHSHISWTQVETGSAITWKYPSCILKGDNSIGEFYSVAM
                     TNHHQQADTGTKMIHIGKNTRSTIVSKGISAGVSQNSYRGQVKVLKSALNARNFSQCD
                     SLLIGDKCGAHTFPYIEVKNSTARIEHEATTSKIGEDQIFYLNQRGISREDAVSMIVN
                     GFCREVLAELPMEFAVEAQKLLSVSLEGSVG"
     gene            15917..16696
                     /gene="sufC"
                     /locus_tag="PKM23_00695"
     CDS             15917..16696
                     /gene="sufC"
                     /locus_tag="PKM23_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNY90015.1"
                     /translation="MLEIKELRVGVDGREILKGINLSVRPGEVHAIMGPNGSGKSTLA
                     QVLAGRHGYEILGGEVRYAGRDLLELEPEERAREGVFLAFQYPVEIPGVNTAYFLRTA
                     LNEVRVHRGQPRLSAGEFLKLVHTKLKLVEMDPAFLNRSINEGFSGGEKKRNEILQMA
                     VLEPRLALLDETDSGLDIDALRIVAGGVNRLRSAENSFVVVTHYQRLLEYIVPDFVHV
                     LYDGRIVRSGDKSLALELEANGYDEIKGNGGRPAVKAEGGA"
     gene            16693..18000
                     /gene="sufD"
                     /locus_tag="PKM23_00700"
     CDS             16693..18000
                     /gene="sufD"
                     /locus_tag="PKM23_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNY90016.1"
                     /translation="MSESSHGSWIDLHREAMRSARPAGPDFLEPLRAAAHARFRERGF
                     PTLRQERWRFTDVSAVAETAWRRAAEGEGETALPLEDLHADPAAYRLVFVNGRFSASR
                     SRLPGAETSFSVQRLSALNDGVLEPTARRMAETAGRTEEPFALINSALAEEGVVITIP
                     DHMAVLVPLEILHYVDGDGIALHPRVVMIVGEQSEALLIESYAGKNGQRYFTNPAADV
                     VVGRGSKLDHYLLQRESASAYHLSYTRATLGREARYRRFALDMGGRLVRHDLEVELDG
                     EGAEASLSGLYMPAGRQHIDHHTTLRHLQPHTFSRQLYKGVLQDKAHSVFNGRIYVAR
                     EAQRTDAIQHNRNLLLSDDALADSQPQLEIFADDVRCTHGGTVGQLDQEGLFYLRSRG
                     IGADLARQIMVHAFAGEITGGIGLAPVRELAEAVVNERLGREE"
     gene            18004..18327
                     /locus_tag="PKM23_00705"
     CDS             18004..18327
                     /locus_tag="PKM23_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNY90017.1"
                     /translation="MVELRDTDDLMDKVVLALREVYDPEIPVNIYDLGLIYEIAVTEL
                     GRVDITMTLTSPMCPVAETLPGEVRNRLEEIAEVREAVVAVTWEPPWDESMMTEEARL
                     ELGLF"
     gene            18338..18667
                     /locus_tag="PKM23_00710"
     CDS             18338..18667
                     /locus_tag="PKM23_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HNY90018.1"
                     /translation="MTEYVTLFAADELEEGGMRRVMAGGEPLVIVRQDGEWFALADRC
                     SHETSRLSEGYLEGGMICCALHGAAFDLRSGEALRPPAYEGIAVHALRIEGGMVQVAI
                     EEENDRG"
     gene            18654..19877
                     /locus_tag="PKM23_00715"
     CDS             18654..19877
                     /locus_tag="PKM23_00715"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HNY90019.1"
                     /translation="MIEVSKIRADFPLFAQPENRDLVYLDSAATTHRPRQVLEAIDEF
                     YRRDNANPHRGAYRLAERATTAYESARSRVAAFIGAGRSEEVVFTRNATEAVNLVAWG
                     WAERHVREGDEIIVTELEHHSNLVPWQMAAQRRGAKLRFLEFDQAGNLDPVQLEGLLS
                     ERSRLLAITQVSNALGTINPLRPFIDKAHAAGALVLVDGAQSVPHTAVDVRALDADFL
                     VFSGHKMLGPLGIGVLYAKIEHLEAMEPFLFGGDMISSVGYHSSEWNDVPWKFEAGTQ
                     NVAGAVGLAAAMDYLDGIGMEAVAEHDRRLTRLAVEKLAPIDQLHLYGPMPERGPVVS
                     FNLATIHPHDLATFLDQKGIAIRSGHHCAQVVMRKLGLAATARASFYLYNDEQDVERL
                     VTGLLQAKDYFKKWL"
     gene            19868..20362
                     /locus_tag="PKM23_00720"
     CDS             19868..20362
                     /locus_tag="PKM23_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNY90020.1"
                     /translation="MALDELYQEVLLDHYKQPRNKGVLAEPDIRLELKNPFCGDEILL
                     TLKMAEGRVAEAAFEGHGCVISQASASVMTEKIKGLRVEEVAAIADQFTHLVREGKYE
                     RPEGEEFDEIEAFAGVCEFPTRVKCAMLAWRTLLQGLKEHEGREHLHDHHVHGAEGEA
                     PNRG"
     gene            complement(20476..21024)
                     /locus_tag="PKM23_00725"
     CDS             complement(20476..21024)
                     /locus_tag="PKM23_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNY90021.1"
                     /translation="MAAAIGLVELSSIAKGIEVADAMVKRALVEVVVNRTICPGKYMV
                     LVNGDVDDVNASIETGVELGGHQVVDHFVIPNVHRSVFPALSGISHTPELRALGIIES
                     FSVASAIEAADAAVKAAAVDLLEIHIAMAIGGKAYVTLTGDVAAVQSAVDAGAAVVKR
                     RGLLVEKIVIPSPRKEILAQFF"
     gene            complement(21049..22386)
                     /locus_tag="PKM23_00730"
     CDS             complement(21049..22386)
                     /locus_tag="PKM23_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNY90022.1"
                     /translation="MEDNSTILRKIRAAGVVGAGGAGFPTYKKLDARVEQIIANGAEC
                     EPMMYKDREVMLQEKELLLEGLSIMQQLTGARRVTIAVKRKNSDVAALLRPDAEKLGF
                     DLFIMEDVYPAGDEYVLVYEITGRQIPPGGLPLHVGAIVNNVETIVNIARAVRGDAVT
                     DKYVTITGAVGEAMTVRIPVGTRFSDLLALAGGTTLNRPVVFTGGIMMGGVENDLALP
                     VVKTNGGLIFLPADHPVAVRKLTPPAQYQRIGHSCCDQCTLCTELCPRYLLGYPIQPH
                     KVMRSLLMTGSEKERYSLWAAYCCECNICSLFSCPEKLDPKNICVDAKKLLREKQISR
                     TTEELKELFLDVHPVRSSREIPITMLYQRLGIKPYDRKAHFRELNFAPAEVELPLQQH
                     IGAPAVPVIKSGDRVVKGQVIAEVAEEKLGCPVHASISGIVAAVSEKSIVIKG"
     gene            complement(22376..23209)
                     /gene="eutJ"
                     /locus_tag="PKM23_00735"
     CDS             complement(22376..23209)
                     /gene="eutJ"
                     /locus_tag="PKM23_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HNY90023.1"
                     /translation="MSALDRVNQRLRRVAALVNNKRRVTGAPPCHLGIDLGTADVVLI
                     ACDESGEPHAAFLEWADVVRDGVVLDYFGAIQIVKSLVAKAEEKLGQHFTTASTSFPP
                     GTDPYACVNVLKAANLNVTGMLDEPSVVARLLEIDRGAVVDIGGGTTGISVVEGGRIV
                     YSADEPTGGRHVTLTLAGNRRISYEEAEAIKRGPDAAQILPIIQPVFEKMADIIRTHL
                     ADRPVEQLYLSGGTCCFPGAADIFAAELPGLEVILPSEPLYLTPLAITMNQTFEHSDG
                     R"
     gene            complement(23218..24627)
                     /locus_tag="PKM23_00740"
     CDS             complement(23218..24627)
                     /locus_tag="PKM23_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HNY90024.1"
                     /translation="MSQQEVARIVEAVVERMRTTTSGDTTGRSALPAGIFATLDEAVA
                     AATAAQKRIGNLEFRDKIILAMREAGARHARELAELAVEETGMGRVEDKVAKNTSQAL
                     RTPGPEVIHAEAISGDHGLTLIENAPWGVIASVTPSTNPAATVINNAISMVSAGNSVV
                     FAPHPAAKRVSQRTISLLNEAVVAAGGPHNLLTAVAQPTIETAQQLFKYPGIGLLVVT
                     GGEAVVHEARRQTDKRLIAAGAGNPPVVVDESADIPHAAACIVSGASFDNNIICADEK
                     EIIVVDSVADQLKREMAKNRAVELDARQAEAVAQVILKEYPGPNPVINRKMVGRDATV
                     IAREAGIQVPPDTRLLFTETDKSHPFAQIELMMPVISLIRAANADAAIDLAIELEHGL
                     HHTAAMHSRNIDHLHRMSREINTSIFVKNGPCIAGLGFGGEGWTSMTISTPTGEGVTC
                     SRTFVRLRRCVIVDHFRIV"
     gene            complement(24652..24948)
                     /locus_tag="PKM23_00745"
     CDS             complement(24652..24948)
                     /locus_tag="PKM23_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006508172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNY90025.1"
                     /translation="MELGKVVGQVVSTVKMEQLAIGKLLLVRFIDEHGKAAGKTQVAL
                     DTIGAGEGEWVLVVRGSSARVSINNATPIDLTIVGIIDTVSSEAGVLYQKSESA"
     gene            complement(25022..25309)
                     /gene="eutM"
                     /locus_tag="PKM23_00750"
     CDS             complement(25022..25309)
                     /gene="eutM"
                     /locus_tag="PKM23_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HNY90026.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGFHQIGSGYVTAL
                     VRGDVAACKAATDAGAAAAQKIGELISVHVIPRPHGDLEDIFPIKMKGEIK"
     gene            complement(25336..25626)
                     /gene="eutM"
                     /locus_tag="PKM23_00755"
     CDS             complement(25336..25626)
                     /gene="eutM"
                     /locus_tag="PKM23_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HNY90027.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGFHQIGSGYVTAL
                     VRGDVAACKAATDAGAAAAQKIGELISVHVIPRPHGDLEDIFPIKMKGTIEG"
     gene            complement(25665..26648)
                     /gene="pta"
                     /locus_tag="PKM23_00760"
     CDS             complement(25665..26648)
                     /gene="pta"
                     /locus_tag="PKM23_00760"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005497796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNY90028.1"
                     /translation="MKLIHLATEYCRWQPRRIVFPDARDERVLRAARYLKDERLAQPI
                     LLGGRFELREFADKVHIPTRGLVILHPQHVPERERYVRILHESRKEKGMTAYEAGQRL
                     DDPLYMGAMLLRDAQADLCIAGNLSSTANVLRAAIHVLGVAPGLRTVSSFFAMISPDG
                     ESVYAFADAAVLPDPTPEQLADIAVATAAQYERLTANEPRVAMLSFSSKGSASHAKVD
                     KMRQATELVKAKAPNLKVDGELQFDAAFVPAVAAKKAPGSPVAGQANVFIFPDLNAGN
                     IGYKLVERLGGFKALGPFLQGLCKPMHDLSRGCSWEDIVEISMTAAYMARS"
     gene            complement(26645..27469)
                     /gene="eutT"
                     /locus_tag="PKM23_00765"
     CDS             complement(26645..27469)
                     /gene="eutT"
                     /locus_tag="PKM23_00765"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HNY90029.1"
                     /translation="MASEYITEGWLREKFGLGWGTEVHLPLDSRLTPAAHQLLEERKI
                     VIKYIDAEGRVFNPDKSGEEAPVQVHPLTSSTGGQKAECLLCHQPVAKKPEALTHLDA
                     NTMVVKNHPRIALRGKIDTLIAHTVLVQIEFDRENKFPWLHPYLADVRSYLGNLLKSE
                     VSGETMIPIALGGADENVVHAVSHNPLKYLGHDHIVPEMSHGRNVALLNLLRAQVREV
                     EVEAARVFIAPDLTVTRPDLIQAANRLSSALYVLMLLTLLAEQGKQLTLEKVVLER"
     gene            complement(27474..28241)
                     /locus_tag="PKM23_00770"
     CDS             complement(27474..28241)
                     /locus_tag="PKM23_00770"
                     /inference="COORDINATES: protein motif:HMM:NF018008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90030.1"
                     /translation="MKKLITAQTIKEACTAGIKELPASVQTTIITPEAATLAAKFGIC
                     FVEPGSESCPLQQRAQAAGGDQELVKAIAAQVIQRLPADKRDPATIARLVAEQLQQPP
                     AACPPSPSPLPVSQPSITAALAPTAPCCQRVVDDSGLIVVRGGSVRMDIMPEAGPGKQ
                     VRLADVITSKDRSPMCAGFMSWRKMDSFAWTLNYDEIDIVLQGTLAITINGKCFEGKV
                     GDLFYIPKGSQIIFGTPTYTKIMYVTYPADWASQKQG"
     gene            complement(28255..28722)
                     /locus_tag="PKM23_00775"
     CDS             complement(28255..28722)
                     /locus_tag="PKM23_00775"
                     /inference="COORDINATES: protein motif:HMM:NF022128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HNY90031.1"
                     /translation="MLNNFILIGGTGVGKSTLFAALLGRLGEVQKTQALVFHDECTID
                     TPGEYFDNPRLYTALISTMTDIQTIVYVHQANSLERKLPFGLFHIYETKQIIGVISKI
                     DLEDARVSEVEELIRDAGIQGPIYPVSVYQPESIDALRRFLSREPFSAVHPGE"
     gene            complement(28722..29081)
                     /gene="eutS"
                     /locus_tag="PKM23_00780"
     CDS             complement(28722..29081)
                     /gene="eutS"
                     /locus_tag="PKM23_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HNY90032.1"
                     /translation="MEDSRNPYQDKERIIQEYVPGKQVTLAHMIAHPGEEVCKKIGIP
                     YVNAIGIMTLTPGETAMIAGDIAVKAANVQIGFLDRFTGALVIHGEVGAVEEALKSVN
                     KTLQEILNYTPCAVTVT"
     gene            complement(29083..29847)
                     /locus_tag="PKM23_00785"
     CDS             complement(29083..29847)
                     /locus_tag="PKM23_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNY90033.1"
                     /translation="MKIVVVINNKGGVGKTTSAVNIAAWCARFGHKTLLADFDPSAGA
                     SRHLGFGPVAGRVKTLCDYLVDASHNLSAAIHPAGDEQLFCLPAEPSLADFYLEVAED
                     AAEAFFISREQIPQEFDLVICDCPPNMGPLAMNALAIADYALIPVQAQYLAVAGLEVT
                     DDMLSKARRHLNPRLRTLGYFVTHFDRRTRSAAEVYDTLKQRYGDLLLPTVIGMNIKL
                     AEAARAGEPILRYAPSARGALEYGELTRALLARMQD"
     gene            complement(29844..>30068)
                     /locus_tag="PKM23_00790"
     CDS             complement(29844..>30068)
                     /locus_tag="PKM23_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90034.1"
                     /translation="AKTPQPEKPKRTGADLSSRALRHIQARPAGSTADELAAALKCDR
                     PAILGVLKTLLDEGVIEKMQRKYYPLTDKK"
BASE COUNT         5655 a         8857 c         9492 g         6064 t
ORIGIN      
        1 cgaccatacc gtcgcgcagg cggatgatgc gattggagtg ttcagcgata tactcctcat
       61 gagtgaccag aatgatggtg ttaccctgtt catgcaggcc ttcaaagatc tccatgatct
      121 cgtcgccggt gcggctgtcg agattgcccg tcggctcgtc ggccaggatg atcgatggac
      181 gattgaccag agcgcgggcg atggcaacac gctggcgctg accgcccgag agctcgttgg
      241 gcttgtggtg catgcgatcg gccaggccga ccttctccag ggcctcctcc gccatcttgc
      301 ggcgcccggc cgccggggtg ccgttgtaga tcatcggcag ctcgacattg tgcagtgcgg
      361 aggcgcgcgg cagcaggttg aaggtctgga agacaaaacc gatctcgcgg ttgcggatct
      421 cggcgagctg gtcatcatcc atggtgctca cattgtgctg attgagcaaa tagtccccgc
      481 tcgaaggcgt atcgaggcag ccgatgatgt tcatcagggt cgatttgccc gagcccgagg
      541 gccccatgat ggcgatatat tcattctttt cgacattgag attgatgccg cgcagtgcgg
      601 ggacctgcac agtgccgatc tcgtaaattt tttcaatctt ttcaagcttg attaacatgc
      661 agggttcctc acttgttctt gtcgtcagtc tctttgcccg ctgccttttt ctccttcacc
      721 tggctgccat ccttgagcag cctggagatg gccttgtagc ttccggttac gatcttctgg
      781 ccttcgctca gccccgagat gatctcgatg ttgttgtcat cgctgatgcc ggtcttgacc
      841 ggcaccgctt ttaccgtgcc gctgttgacg acgaaaacca cctcagtcat ctcgtccttc
      901 tcgtcttcct tcttctcgcc cgactcggtg ggcggattcc cggatttacg cttccctccc
      961 acattcctga gcgaatcggc cttgaggtcg cgggcagtga ctgcctggat gggtacgttg
     1021 agaacctggg ggcgggtctc ggtgaggata tccacagtgg cggacatgcc ggggcgcagc
     1081 aagtcgacgt tggaggtgat gccgatcttg acgtcgaaat tggtcacctg ttcctgggtc
     1141 cccgccccgg tggtggtggc gacgtgggcg atctcggtga ccttgccctg gaaggtggtg
     1201 tcgcgaatgg cgtcgacctc gattgtggtc ttgtcgccga gatgaagcag tacaacctca
     1261 ttctcgtcga tttcggagag gacctccata tccttgaggt tggcgagatt catgatgata
     1321 tcagcctgga actgcgagcc caccgcgatt tcaccctcct ccttgttgag cttggtgacc
     1381 gtgccgtcaa tgggtgaggt cagtcgggtt ttgccgaggt catccatcgc ctgtttgagg
     1441 gcggcccgcg tctggtcgag gctgctcgcc gccgactgcc gggtggcttc cgcagcgtcg
     1501 agctcggcct gcgaggcgag gtttttcccg aaaagatcct tgatacgggc ataatcggca
     1561 tcggcctttt tcacccccgc ctttgccgaa gtgagttgcg actggatctg ctcgacatag
     1621 gccgcaaggc tttcacggtc cagttcgacc agaagctgac cctgcttcac ggcatccccc
     1681 tccttgacgt ggatcttttt aatctcggcg gagatgcggg cagcgatctt gatgtcggtt
     1741 accggctgga ttttgcccga cccggaaacc gtccggctga tatccccccg tttgacctcg
     1801 gctgaggtga tttcgatgac ttcaccgcgg cttttacgca ggttgaccag gaccatcgct
     1861 gcgatgatgg tgacagcggc cacagcgatg atgattttca ttttattgga catgaggaga
     1921 ctccctatca gactggctgg gtttgttcac aatccggctt cggcggcgcc catggccgtc
     1981 tccagccggg ctctcgcaat cttggcttca tattcggcgt tgaccaggtt ggcctgggcg
     2041 cgggtcagct cgacctgggc ctgggtcacc tccaactccg tgccggaacc gacgcggcgc
     2101 ttttcctgct gcaggcgcag gttctcgcga gcggcctcga tgttctgctg gttgatctcg
     2161 atgaaatcct tgtaggcctc gagctgcagg aaatactgct tgacctgagc ggtgaggagg
     2221 cgcttctgct cggccagatt ctcttcgtcg atctggtagt tcagtttggc gcgctgtacg
     2281 ccggccttgt cggagaggcc gttgaagatg ttcaggtcga tccccacgcc cagggtagcg
     2341 ctgaaatcct ggtccaggtt gctcgagaag acgcggctga gatcctcatt cgagcgccca
     2401 tagctgatgc ttcccccgat gctgggggca tagcgcatct gcgccatctt gaggttgaag
     2461 aggctggccc gggactgata ttcataggcc ttgagagcgg cgttgttctt gattgcttca
     2521 ttagcggcat cggcgaaggt gatggccggg aagatgggct ggctctcatc ctcgcgcacg
     2581 ctgaccggaa tgtcaggatt gcggccgagg gcattgttaa ggtcggcccg agccgcgtca
     2641 accgtgttct gctggttgat cagggtggag cgggaggagc cgagggtgac cctggcctga
     2701 aagacctcag actgggagga gatgccgatc tccatcatgg tctgggcgcg ctgaacctcc
     2761 tcttcggcca gccggacagc ctcctgatag acctgttcaa gcttgatcgc cttgagcaga
     2821 ttgtagtagg cgtccttgac gttgtagatg accatctggc gggtgttctc catggtctgg
     2881 gcgctcgcct gcttgttggc cttggccgac ttgatgctgt acagggtctg gccgaaatcg
     2941 aagatgttct ggttaagcga aatgcgcgcg gaatggttgt tgcgctcggt ctgctcctgg
     3001 tagatctcct gctgggtgta gatggtgcgc ccggtgacgg gatcgatccc cgtgggcacg
     3061 tcggcgatat caacgcgcgc gccctggatg tagcggccgg tgttgaagga gctgttgatc
     3121 tggggcagcc aggcggcggt ggccgtggtg acattggtcc cggcgatctc catgcttttg
     3181 tgggcgacgc gcagggtgga attgttctcc agggcgatgg cgatgcactg ctgcagggtc
     3241 aagggctccg gcgccggctc ctgggcgaag gcgctgccca gacccagcag aatgagaaga
     3301 atcagcgaag tgaacttgtt catgaaaggt actcccgttg gaagcgattg gttagagcct
     3361 atgagaagag gccgcccatg gccgcggatc ccagatacca aaggaccagc agtccgacca
     3421 ctagtggcca cactttttta accttgagcc cggcgaccac ggcgatgccg atacagagca
     3481 cgccgatgta ccagatgttg aagaggtcgg tgttcatcag gaacttgtag ataaagctct
     3541 ccctggccga gtcgggcatg aaagtggcca ggctgaagtg gacattcatg gtctccttga
     3601 agcgcatgat cggcgccttg agcagcatac ccaccgaggg gatgagcata ctcagggaga
     3661 ccacctcgag catgtgcttg taggtggtgg tgccgccgag gagggtgcgg gcgacgaaaa
     3721 gccatacccc gcccatcacg agaatgaccg ccaggccgcc gatgagggcg gaaccgtaca
     3781 tgacgtattt catcaccggg ctgttgattc tctcgatggc gctgtcgatc tgctcctggt
     3841 ccaggccgcg attctccatc tgggtgacca tcttttcgtg ctgctccttc tggatggcgg
     3901 gggcgatata gatggtcatg atcagcgaaa gcagcagcac gagcacggtg ggggcgatcc
     3961 agcgcggatt ttcacgcaca gccgcaaaac tcttgctcgg ggccgcaaag acattgataa
     4021 tgcgctgcag cagacccatg ttgccctcac tggcggaggg gacgcccgaa gtggaatact
     4081 cttgcatggt gaggcctcca atgtgatgta aaaagcaact aataacaaag acgtatcatg
     4141 ggtgtaaaag tttcgcttta ttcgcccggc ccggcgataa gttcgtcctt ccgttccatc
     4201 atgtaagtaa aggcggcatc gtggtcgttc gggatcaccc cctccaggat ggcctcctcg
     4261 atggcctttt tgatcttgcc caccgcggga cccggctgca gatcgagggt cgccatgatc
     4321 tcttcgccgc ggaccggggg ctggaaagcc cgcagacggt ccctctcctc aacctcctgc
     4381 atgcgctcga ccacgaggtc gaaattggcc aaatggctgg ccacgcgttt tgggttgccg
     4441 gaggtgatgt ccgcacggca gagggtgatg agatcatcga gctcggcccc gccgagaaag
     4501 atgaggcggc ggatggccga atcggtcacc ccctcctcgg cgagatggat gggacggaga
     4561 tggagccgga tcagcttttg cgcataatcg atcatctcct gggagaggcg gagccgcctg
     4621 ccgatggccg gcagcatgcg ggcccccagt tcctcatggc cgtgaaagct ccagcccaca
     4681 ccgggcttga actctttggt ctggggcttg gcgacatcgt gatacagcgc agccaggcgc
     4741 aggccgggat ggtccgagac ccgcgccaca ttgtcgacca ccttgagggt gtgctcgaag
     4801 acatccttgt ggcggtagcc ggcgacctgg tcgaccccct tgagggcgct gatctcgggc
     4861 aggatcagat cgagcacccc ggtgtcgttc atgaggtgga agccgagcga gggctttgtg
     4921 ctggcgagga ttttcatgag ctcctcgctg atgcgctcct gggtgatgat gcgcagccgc
     4981 tcccgttcgg cggcgatggc ctccagggtg acgggttcga tgtcaaactg gagctgggca
     5041 gcgaaacgga tagcgcgcag gatgcgcagg ggatcatcgc tgaaggtccg gcgcggctcc
     5101 aggggggtgc ggatgatgcc ggctttcagg tcggcgcggc cgtcataggg atcaatgagc
     5161 tcgccccggt cggcgggatt gaggctgacg gcgatggcgt tgatggtaaa gtcgcggcgg
     5221 gcgagatcgg tgagcagatc agcgctgctg acctcggggt tgcgcgactc gccgcgataa
     5281 ctctctttac gcgccccgac cacctcaatg actgtactgc ggtggcggat catggcggtg
     5341 ccgaatttct tgtagaccgt gacatttttc acccgcaggc ggtcggcgag caggcgggcg
     5401 aaggccggac cctctccaac cacgacaatg tcgatatcct tgccctgctt gcccaggagc
     5461 agatcgcgga catagccgcc gacggcgtag gcggggatag cggagcgctc cgccacctca
     5521 ccgataattt ttatcagagc ggtgtcgaaa agtctgttat tctttttcat ttttatgtaa
     5581 tttagtaggg attccggcgg cccggcagcc tgagagcggc cgtcagaggg tcagttataa
     5641 tgtacaaatt cgggggtgaa aataaaagcc aaaagcgcga gtacccggct ggtatttgac
     5701 cgaaatggtg taattccagc cagatgaccg cgactgcagc gagcgctgtc cgccggccgg
     5761 ggggagatgt ttcatggccg tacaatgttt caccagaaca cgaatgcggg gggtggcgtt
     5821 gaataattgg ctttaaatcg ccataaatga tggggttggc cgggatcagg gtggccaggt
     5881 taaatcatta aataacaata gaagcatgct gcgtttgctc agccattgtg acggaaatct
     5941 caattgccgg tgaaaaatac ggacgatgtc aaacatggca ttgaatttgc gatcaagcct
     6001 gcagtccata ttatgctggc aatattgcgg cgcccggctt tcgggctgca aaaccgggtg
     6061 cggcacaggg ccgtcatcaa aaaagaacca aatggaggcg aaaatgaagt catctcccat
     6121 gctacgcatc ttgttcgtcc tcgcgctgat gctaggggcg gcaggtgatc tctccgcgca
     6181 gattctgcgc cgtgagactg ccgatcctct gcagacgggc actgcaagct ggatcaaagt
     6241 cgaattcagc attgccggag gcagcaggac ctatttgaac gacaacttta ccggcacgga
     6301 attccggatc gacctcaacg tcgacggcga tgacaatcct agtaccaacc tcggagacct
     6361 ctacaccctg cgcgggccgg ctgaaatcta tacgacgcct ccgggagtcc cggggggcgc
     6421 taaagatgcg gtcatctacc ttcccatccg ccagggctct tcgaccccat ttccctacga
     6481 caccgacctg cgcccctgga tctggctggt cgagggcggc ggtctgcaga tcagcggcca
     6541 gagtgtttcc ggcccctggg gggtgatcca gtatccctcg gtctacatcg aatgcgatga
     6601 cggcatcagc ccgaccatcc ggcatgccta ttattacgat gacggcagcg gtcattcccc
     6661 cgccgtgtcc ggttcgcatg tgggcaacca gacacccttt gacggttaca tcgaccgcgt
     6721 tgatctggag tggagcgagc cgatgagctc ctccaacgtc accgtcaatg accagatttt
     6781 cggcggcctg ggcacgacga ttcacagtat ggaatcgatc ggcagctgga acatgatcgg
     6841 cccctcctgg aagcgtttca ccctttgggt gcgttccagc gctccgaaca gcggcatctc
     6901 cccgatcctg acctataccc agcctctcag cctcaccgac cgcttccgcg aggcctcgac
     6961 cgggcagtat caggcctatg ccgccagcca tgcccgtgcc ctcacagaca aggcgggtcc
     7021 ggcgatcatc agcgcccata ccaagcgcgc gatgcgtcgt cagctcctgg ctgccgctct
     7081 ggccgaccgg cgcatcgagg tgaccttcag cgagccgctc gatttcgcca gcgtccaggg
     7141 ggtcgatttt acagtcaata ccaccacggc cagccccaat cccatcaccg ccatcatctc
     7201 gccgacctcc acgtcgacct ctccgagtgc ggtatgggag ttccagctca ccacccccta
     7261 tgcgaccgga gatgaggtcg gcgccattca gtatgtgtta gaccgtcagg tcaccgacgt
     7321 cgtcgccaat tataatggcg aatcgttggc cccggcgccg cccacagccc cctccaacgg
     7381 gccgggattg atcattccga tcaccgatgg catcttcccg atcatcaccc acgtgctgac
     7441 ccacgacgcc attctgccgg gtgatctgac caccggcggc accaatggct ggggttatct
     7501 cgactatgtc gatgtactgt ttgaccatgc catgaacacg gcccgtgtca gcactgccgg
     7561 cttgcaggtg accggagagg gcatcgtcac catcgccggg accggcacct ggatcgcccc
     7621 caacaccctg cgggtgatcc tgacggcgac cacccccagg gtggccaaca ccgggcttat
     7681 cccgcgcgta acctatacca atccgggcga tcccaacggc ctcaacgaac cggtcaatct
     7741 cggtctcgcc gaagccctct acagctccga catcaccctc tccccgacca acggccaggt
     7801 tgtgcagatt gtggataatg ccgggccggc catcatccgc gccctgactg cggggaccaa
     7861 gcgcatccgc ctggtcttca gcgagaaggt caacacctca agctggccga tcaacccgac
     7921 gccgggactg ccctcgcccc tgttcaagtg gttcgtcggg ccttccaatt acgacgtctc
     7981 cggcattcag gtctacttca ccggtatgac cccctcccgc cgggacagta ttgtttatct
     8041 gaatcatacg ggattggcct ggaccaagaa cgacagcggc gccatcaatt accgcgcgca
     8101 gggggtggtt tgggatctgg cctcctcgtc gaacggcaac ctgcagtatg acgatgatgt
     8161 cagtctcagc ggcacgacac ggcgtctgct gggttcggat gtcaaggtgg aacgcgacaa
     8221 tatcccaccc atcctgctgc gactcgagac cgtggatatc gatgcggacg gcaagctcga
     8281 tcattaccgt ttttccttcg atgatctctc accgatctat cccaagcgct catttgtccc
     8341 gggcaactgg accatcaccg ggtatgacgg cctcaagagc aacctcggcg tcaacctcaa
     8401 tgtctacaat cccggctacg cctactatca gccgaatgcc atcaacgcct acggggatac
     8461 ggtcgcggcc tatgtcgctt tcaacgagac cactgccggc ggcccggccc tcacccccta
     8521 cggtggagat accggggatg taccggatgt cgtcgttctg gccaacaacg gctttgccga
     8581 ctgggcggac aacccgatgg cacccttgcc ggtgggcatc actctggaac gcgacgatgc
     8641 gggtccggcc attatgagcg ccaagaccat cagccgtttc gacgtcgagg ccttcatgag
     8701 cgaggatctc gccgactggt cggtcttcag ctccgatttc ttcctgaaca tggcccccaa
     8761 cgccaactat ttcggcgcct ggccgatcaa ggaggccaaa gaggttatcc ccggcaaggt
     8821 gctgatcaac accttcttct acagcagctc cctgggctgg gatcccaaga gcgagggcat
     8881 cctgcgcttc gacagccagg gcgtcgtcga ggatgtggtc aatcctgtca ccaacggcaa
     8941 catccagacc aattgggtga cggtgaccag caatgccgcc tgcacctttg tgattcagcc
     9001 ggtggtctcc ggcgcccagg tgcgcggtgt gcccttcgac gtccaggtca tcgcccgcga
     9061 caagtacggc aacgtcgaca ggaactttgc cggttatctg accttctcga ccaatctgca
     9121 gcaaaacgag atcgtcctgc ccagcggtcc gaagaagctg atcgatggca tcggcacctt
     9181 ccgtttcacc agctgggtga acaccaccga tctggtcatc accgcttcga tcgccacgga
     9241 tgtctatccc cttgatgccg cctccagcgg tccggtcact gtgatcgatc cgatcatcga
     9301 ccagccggat tatctgacgg tcgaggatgt acccggcgat cagggtggtt ttgtcaccct
     9361 caagtggccc ttctcggcta accatcaagg catgggtgcc acgccggtga tcaattacta
     9421 cgagatcttt tataccatca agggcagcga caccctgcac tactggccgc agcagattgc
     9481 cgcctacaat ccctccggca caggctccac gatgatgacg gttgatttcc cggtcgtcac
     9541 cagcgactcg atggccttct acgtccgtgc ggtctgggtg cctcccaccg ccgcggttaa
     9601 gggcggcgcc accctctcga tcatggctgc gaaggcgggt gatcttcccc tggtcattca
     9661 caccctgggc gaggagagcc cggtcacctc ggtgctcggc gcgacgggag cctctgtggt
     9721 ctccggtacg gccatggcca aggggcgggc ggtggacaat atcgcaccga tggcgcctga
     9781 gcgcatggtt gctgcccggg aaggcgcggc cgtcaagctg cactggggcc cggtcaccaa
     9841 aggcgtgaac ggcaccctcg aacgtgcggg cgctatcgcc tacaaggtct atatgcatga
     9901 gaccaatccc tacttcaatc cggatgctga gggaaccctg ctggcgacca cggccgatac
     9961 cagcctgatg gtcaactccg agtccctgcg tcagtactat gtcgtccgcg cggtcgacgc
    10021 cgacaaccat tccggcatct cccgccgggt gggcaagtac ggcttcagcc tgatcacggc
    10081 cgccaagccg agatacaact acatctccct gcctctcgag accgaaatca gcgatgcccg
    10141 ggagcttgcc gaggcgatcg gtgcgggagt caaggtcgtg ctcaggatcg atccggtcac
    10201 caacggctac agcacctact atctgcctga cctgaactac ccggccaccc cctttgcgct
    10261 ccacgagggc atgccggttc tgatccaggc tgatgagagc gcgccggcca gctggttcta
    10321 ctgcggcgca gtccctgaag cgggcgagtt ccagttcgct ctcaaccggg agcacaagtt
    10381 cagctacaat gaaatcatcg tgccgctcaa ccgctcggac atcaccaatg ccgccgagct
    10441 ggccgctgag atcggcggag tggaagtgct gcttaaaatc ggcgccgacg gcagaggttt
    10501 caccaactac tggctgccgt cgctcaactt tggcaatccg atgacgccgt tcagcgtcgc
    10561 accgggtgaa ccggttttga tccaggtcaa caagaccgcg ccggctctct ggcccacgta
    10621 tactcgataa tcaggaggga tggtcatgaa acgcaaattc acctatattc tgttcggcct
    10681 gctgcttctc ggcctgtgcg gggcggccct gccgcaggaa ccgcacatca tcggcggcaa
    10741 gtttctcaat ccggatggtt cagtgcccgc cgggggcgaa atccgtttcc aggcctacct
    10801 gcaaaagtca cccggggaca ccagcgcagc cagctggtgt tccaccggcg gaggctggag
    10861 cgtcgaggtc aagcagaccc cgatgatctc ctatgccaac tgggaggccg gcgacaccct
    10921 ggtagtcgtc tttgaaaatg tcctggaaac gagcccctac tacggggcgc gcaaggtggt
    10981 caagcatgcc accaccgagg attctccgga attcgtgggc agcatctccc tgccggttga
    11041 actcaccacc ttcacggccg cagtaaagaa cacctccatc agcgatgtgg tgatgctgga
    11101 atggcggacc gtgggggaga gcaacaacct cggctttgag gtcgaacgca gcctggacgg
    11161 caaaaacttc agcaagctcg gttttgtcgc cggcgccggc tcgaccaatc tggcccaggg
    11221 ctatgactac cgggatgacc gggtggaggt gggcacctat ttctaccgcc tcaaacagat
    11281 cgaccgcaac ggctccttca cctattcgga tatccgcgag gtggaagtca cggccccgca
    11341 gcgctacgag ctgacgcaga atttccccaa tcccttcaat ccgcagaccg acatcgtttt
    11401 ccgggtcaag gaggagggcg tggtgactct gaaggtcttc gatctgctcg gcagggaggt
    11461 gaaaactctg gtcaacgaga aaaagggcgc cggcgtccat cgcatcactt tcgacgccca
    11521 tgcccttccc tccggggtct atctctacag catcacgatg ggcagtttcc acgatgtgaa
    11581 gaagatgctg ctggtcaaat aggcgccgcc gctgcaggcg ccgtgaaatc tccaggtagg
    11641 cgatcagacg gtccctcccc gtctgaatgt cctacgcagc ccggcgtcac cggatgcttt
    11701 cagctccggc ggcgcctgag agagcgggac ccttcggggt cccgcttttt tttattatag
    11761 gaacccactc tccggttgtg cgggcaaaaa agcccttgca tatttgccgg cttgttgcta
    11821 tatttattgg ttttactaga aatataccta taattttatc aataagggat tttcgctatg
    11881 gctttgatga agagcatgcg caacagcatg aagaccattc tgcttattct ggtcttcgca
    11941 tttgtcctga cgatcgttgt cgattggggc atgggcggct ttaagagcaa tgcgcctcag
    12001 ggagtgattg ccaaggtcga cggccagtcg atccactatg aagagttttc cagccgcctg
    12061 cagaacgagc tggcagccta tcgggagcag tcgggcagcg atcccgaggg ctatcagctc
    12121 cagcagcttg agacgcgcgt cttcgacaac ctggtgcagc agcgccttct caacgaagag
    12181 gtcaaggcgg tcaaactgaa tgcctcggac caggagatca tcgacgagat tttcaacaat
    12241 cccccgctgg agctgcgtca gaacgaagcc tttcaggact ccaccggggc ctttgacatg
    12301 cagcgctatc aggccgcact taacaatccc ggcgcgggtg agttctgggc ctcggttgag
    12361 gagtatctgc gcacgaccct gccgatgcgc aagctcgaca atcttctgcg cgccaccgcc
    12421 gtggtcagcg accaggaggt acgcgcagaa tacatgaagc gccagctcaa ggcccaggcg
    12481 gagtatattc tggtcccggc caacgatttt ctcgccacgg ccgccaaccc gactgatgcc
    12541 gagatcaagg cctatcacaa acagcatgag gaggagttca aggaggcgga gaagcggatc
    12601 atcgagtatg tcctgctcaa ttttgtggcg accaaagccg actcccaggc tgtcgatgaa
    12661 caggctgccg agcttctcgc cgaggcccgc ggcggtgcgg atttcgccga actggcccgt
    12721 ctgaactccc aggatccggg ttcagccccc cagggtggtg acctcggttt cttcgcccgc
    12781 ggcgccatgg tcaaaccctt tgaggaggcc gctttctccg gcaagccggg cgatattatc
    12841 ggcccggtcg cctcgcagtt cggcatccat atcatcaagg tcggcgagcg caagcaggaa
    12901 gagggagagg aaaaggtcca ggccagccat atcctgctca agtatgagat gtccccgcgc
    12961 acccgcgagg ccctgcagga ggaggccgcc tacatcgccg aggcggccaa ggagcaggat
    13021 ctcaagagcc tcgccacagc cgaaaacatc aagctggagc gcaccgaacc cttcgctgcc
    13081 ggcggcttca tccccggcat cggcatggaa cccaggctga gcggctttgt ttttcgcagc
    13141 aaggaagggg cggtcagcga tgtcatctat gctgaacgcg gctacatcgt cgtccgcgtc
    13201 gccgatatca tccccgaaca catcaagtcc tggagcgatg cccgcgagac catcaccgcc
    13261 aagctgaaaa atgaaaaggc gatggagctg gccagacaaa agtgccagac cctctacgag
    13321 caggccaaat cgaccggatc cctcgccggc gcggccgccc agatcggtgc ccaggttcag
    13381 caggccgccg atttcacctt ctcgagctat gtcgccagcc tcggccgcga agcccgcttt
    13441 gtcggcgccg ccttcggact ccagcccggc gccatttccc agccggtcga ggggacgcgc
    13501 ggctactatc tgatcaaact gctgagcaag aacagcttca atgagcagga gttcgaggcc
    13561 cagaaagagt cgctgcgcgc acagctgatg cagcgcaagc agcagagcat cttcgggcag
    13621 tggtataccg gcctcaagga gaaggcggat atcaaggatt tccgcaagga ttacctctag
    13681 gcgcctgaag ccgatcggcg tcgccgatcg gctcacccgc cgtttcagcc tgcacaacca
    13741 tcatgcggcc ccgatgcccc cggcatcggg gtcgtctctt tttacgccgt acaagcctga
    13801 ctcccacctc attgtcttcc cgcatggcag cacattcagc acgcgggcag ccggctgaag
    13861 acggcgactt taacagccgg ttccgcgccg ccttacttgt gatccgggcc aagatgcctg
    13921 accggtaaat ttatcaccgg tcgggaacaa aatccccgca gggggtgtta ctgtttttaa
    13981 ataagactaa aaaagtccta tatagatgtt caaactttcg aaaaaaaccg aatacagcat
    14041 catggcgctc cgccatctcg ggcgcaatga atcgatgacc gcgactgtcc gggaaatcgc
    14101 tgcggactgc cgcatccctg caccgctgct ggccaagctg ctgcagcagc tcgcccggag
    14161 cggcctggtc cactccttgc agggagtcaa cggcggttac cgtctcaaga gcgccatggc
    14221 ggggataagc ctcgctgagg tgatcgaggc cgtcgagggt cctttccggg tcaccgcctg
    14281 cggcggggaa gcaagccggt gcagccgttc cggtttttgc gacctgcgca gtgcgatagc
    14341 gcccgtgcag gggcagatgc tgagttattt acaaaacgtg accctggccg acctgcacgg
    14401 ttcacggggc gaagaggatt gaatgagcac cgaaatgaac cagcttgaat cgctggccaa
    14461 ccaggaatac aaatacggat ttgtctccga tatcgacgcc gaggccctgc ccagggggct
    14521 gaatgaggag attatccgca cgatctcggc caaaaaacgc gagcccgcat tcatgctgga
    14581 gtggcggctc aaggcctttc gccattggca gacgatgacc gaaccgcgct gggccaatgt
    14641 caagttcccg cccatcgact accaggatat catctactac tcggccccga agagccacag
    14701 cgggcccaag agtccggagg agatcgatcc cgagatcctc gccctctatc agaagctggg
    14761 catccccctc gaagagcagc aggcgctggc gggggtggcg gtcgatgcgg tgctggactc
    14821 ggtatcggtg gcgaccactt tcaaggaaaa gctttcgcag ttggggatta ttttttgttc
    14881 cttctccgag gcggtgcaga accatcccga tctggtgcaa aagtatctcg gcatggtggt
    14941 gccatcgacc gataattttt ttgcggcgct gaactcggcg gtattcagtg acggctcctt
    15001 tatctacgtg cccaaagggg tgcgctgtcc aatggagtta tcaacctatt tccgcatcaa
    15061 cgccgccaac accgggcagt tcgagcgcac cctgatcatc gccgaagagg ggagctacgt
    15121 cagctatctc gagggctgca ccgccccgat ccgcaaggag aaccagctgc acgcggcggt
    15181 ggtcgaactg atcgccctgg acaatgccga gatcaagtac tccactgtgc agaactggta
    15241 tcccggcgac aaggagggtc gcggcggcgt ctacaatttt gtcaccaagc gcgggctctg
    15301 cgccggagtt cattcgcaca tctcctggac ccaggtcgag accggttccg cgatcacctg
    15361 gaaatacccg agctgtatcc tcaagggtga caactccatc ggggaattct attcggtagc
    15421 catgaccaac catcatcagc aggccgacac cggcaccaag atgattcaca tcggcaagaa
    15481 cacgcgcagc accatcgtct caaaggggat atcggccggt gtgagccaga acagctaccg
    15541 cggccaggtc aaggtgctca agagcgccct caacgcccgc aatttttccc agtgcgactc
    15601 gctgctgatc ggggacaagt gcggagcgca caccttcccc tacatcgagg tgaagaacag
    15661 cacagcccgg atcgagcacg aggcgaccac ctccaagatc ggcgaggatc agatttttta
    15721 cctcaatcaa cgcggcattt cccgtgagga tgcggtctcg atgatcgtca acggtttttg
    15781 ccgggaggtg ctggcggaac tgccgatgga gttcgcggtc gaggcccaga agctgttgag
    15841 cgtcagtctg gagggaagcg tgggctgagc agcctgtgct cttttatatg gaaccaatca
    15901 gagatggaga aaaataatgc ttgaaataaa ggagctgcgg gtgggcgttg acgggcggga
    15961 gatcctcaag gggatcaacc tgagcgtccg tcctggcgag gtgcatgcga tcatggggcc
    16021 gaacggttcg ggcaagagta cgctggcgca ggtgctggcc ggccggcatg gctatgaaat
    16081 tctcgggggc gaggtgcgtt atgccgggcg tgatctgctc gaactcgagc ccgaggagcg
    16141 ggcccgagaa ggggtgttcc tcgcctttca atatccggtg gagatccccg gggtcaacac
    16201 cgcctatttc ctgcgcaccg ccctcaatga ggtgcgcgtc catcgcggac agccgcggct
    16261 gagcgcgggt gaattcctca agctggtcca caccaaactg aagctggtgg aaatggatcc
    16321 ggccttcctc aaccgttcga tcaacgaggg tttttcgggc ggcgagaaaa agcgcaacga
    16381 gattctgcag atggccgtcc tcgagccgag gttggccctg ctcgatgaga ccgattccgg
    16441 gctggatatt gacgctctgc gcatcgttgc tggcggggtc aatcgcctgc gcagtgcgga
    16501 gaactccttt gtcgtggtga ctcattatca gcggctgctc gaatacattg tccccgattt
    16561 tgtccatgtg ctctatgatg gccgcattgt ccgctccggc gacaagtccc tggccctgga
    16621 actcgaggct aacggctatg acgagatcaa ggggaatggc ggccggccgg cggtgaaagc
    16681 ggagggcggg gcatgagtga aagcagtcat ggctcctgga tcgatctgca tcgggaggcg
    16741 atgcgttcgg ctcggccggc tgggcccgat ttccttgagc ccctgcgggc ggctgcccat
    16801 gcgcgcttcc gcgagcgtgg ttttccaacc ctgcgtcagg agcgctggcg ttttacggat
    16861 gtctctgctg ttgcagaaac ggcatggcgc cgcgcggccg agggggaggg cgaaactgcc
    16921 ttgcccctgg aggatctgca tgcagacccc gcggcctacc gtctggtctt cgtcaacggc
    16981 cgcttcagcg cgagccgctc ccggctgccg ggggcggaga cctcgttttc cgtgcagagg
    17041 ctttccgcgc tgaacgatgg ggtgctcgag cccaccgccc ggcgcatggc cgagacagct
    17101 gggcggacgg aagagccctt cgctctcatc aacagcgccc tcgccgagga gggggtggtg
    17161 atcaccatac cggatcacat ggctgtgctc gtacccctgg agatcctcca ttatgtggat
    17221 ggtgacggga ttgcccttca tccgcgcgtg gtgatgattg tgggcgagca gtccgaggcg
    17281 ctgctcatcg agagctatgc cggcaagaac ggtcagcgct atttcaccaa tccggcggcc
    17341 gatgttgtgg tcggccgcgg gagcaagctg gatcactatc tgctccagcg cgagagcgca
    17401 tccgcctacc acctctccta tacccgtgcc acactcggac gcgaggcccg ctatcgccgt
    17461 tttgccctcg atatgggcgg gcggctcgtg cgccacgacc tcgaggtgga actggatggt
    17521 gaaggagctg aggcctccct ctccgggctt tatatgccgg ccgggcgcca gcacatcgat
    17581 catcacacca ccctgcgcca tctccagccg cacaccttca gccgccagct ttacaagggt
    17641 gttttgcagg acaaggccca ttcggttttc aacggccgga tctatgtggc gcgcgaggcc
    17701 cagcgcaccg atgccatcca gcataatcgc aacctgctgc tttcagacga cgccctggcc
    17761 gacagccagc cccagctgga aatttttgcg gatgatgtcc gctgcactca tggtggcacg
    17821 gtgggtcagc ttgatcagga ggggcttttt tatctgcgtt cgcgcggcat cggtgccgat
    17881 ctggcgcggc agatcatggt ccacgccttc gccggtgaga tcaccggcgg gatcggcctt
    17941 gccccggtgc gcgagctggc tgaggcggtg gtgaacgagc ggctgggccg ggaggagtga
    18001 aggatggtgg aattgagaga cacagatgat ctgatggaca aggtggtgct ggccctgcgc
    18061 gaggtctatg atccggagat tccggtcaac atctatgatc ttggcctgat ctacgaaatt
    18121 gcggtcactg agctggggcg ggtggatatc accatgaccc tgacttcgcc gatgtgcccg
    18181 gtggcggaga ccttgccggg cgaggtccgc aatcggctcg aggagatagc ggaggtgcgc
    18241 gaggctgtgg ttgcggtcac ctgggagccc ccctgggatg agtcgatgat gacggaagag
    18301 gcgcggcttg aactgggctt gttttaatga ggtgcgcatg acggaatatg tgacactgtt
    18361 tgcggccgac gagctggagg aagggggcat gcggcgggtc atggccgggg gggagcccct
    18421 ggtcatagtc cgtcaggacg gggagtggtt cgctctggcc gaccgctgtt cccacgagac
    18481 ctccaggctt tcggaggggt atctggaggg tggaatgatc tgctgcgccc tgcacggagc
    18541 ggcctttgat ctgcgcagcg gcgaggcctt gcgtcccccg gcctatgaag ggattgccgt
    18601 ccatgcgctg cgcatcgagg ggggaatggt tcaggtcgcc atcgaggagg agaatgatcg
    18661 aggttagcaa aatacgcgct gattttccgc tcttcgcgca gccggaaaac cgcgatctgg
    18721 tttatctgga cagtgcggcc acgacgcacc ggccccgtca ggtcctcgag gcgatcgatg
    18781 agttttaccg gcgcgacaat gccaatcccc accgcggggc ttaccgcctt gctgagaggg
    18841 cgacgacggc ttacgagtcg gcgcgcagcc gtgtggccgc gttcatcggc gccggccgca
    18901 gtgaggaggt cgtcttcacc cgcaacgcca ccgaggcggt caatctcgtc gcctggggct
    18961 gggcggagcg ccatgtccga gagggcgatg agatcatcgt caccgagctg gagcaccaca
    19021 gcaacctggt gccctggcag atggcggcgc agcggcgcgg ggccaaactg cgtttcctgg
    19081 aattcgacca ggcgggcaat ctcgatccgg ttcagctgga ggggctgctg agcgaaagga
    19141 gccgcctgct cgccatcacc caggtctcga atgccctcgg tacgatcaat cccctgcgcc
    19201 cctttattga caaggcccat gccgccggag cgctcgtcct ggtggacggc gcccagagcg
    19261 taccccacac ggctgtcgat gtccgcgctc tcgacgccga ttttctggtc ttttccgggc
    19321 acaagatgct cgggccgctc ggcatcgggg tcctctatgc caaaatcgag catctcgagg
    19381 cgatggagcc cttcctgttc gggggcgaca tgatcagcag cgtcggctac cactcctcgg
    19441 agtggaacga cgtcccatgg aaattcgagg ctggcaccca gaatgtcgcg ggcgcggtcg
    19501 gactcgccgc ggcgatggat tacctggacg ggatcggcat ggaggcggtc gcggagcatg
    19561 accgcaggct gacgcggctg gcggtcgaaa agctggcgcc gatcgatcag ctccacctct
    19621 acgggccgat gcctgagcgg ggaccggtgg tctctttcaa tctggccacc attcatcccc
    19681 atgacctcgc cacctttctt gaccagaaag ggatcgccat tcgctccggc caccactgcg
    19741 cccaggtggt gatgcgcaag ctcggtctgg cggcgacggc gcgggccagt ttttatctct
    19801 ataatgatga acaagatgtg gaacggcttg tcacgggcct gctgcaggca aaggactatt
    19861 tcaaaaaatg gctctagatg aactctatca ggaagtgctg ctcgaccact acaagcagcc
    19921 gcgcaacaag ggggtgctgg ccgagccgga catccgcctg gagctcaaga atcccttttg
    19981 cggggatgag atcctgctca ccttaaagat ggcagaggga cgggttgcgg aggccgcctt
    20041 tgaggggcat ggctgcgtta tcagccaggc ttcggcctcg gtgatgaccg agaagatcaa
    20101 gggcctgagg gtggaggagg tcgccgctat tgccgaccag ttcactcatc tggtcaggga
    20161 gggaaaatat gagcggccgg agggggagga attcgacgag atcgaggcct tcgccggggt
    20221 gtgcgaattt cccactcggg tcaagtgtgc catgctcgcc tggcgcaccc tgctgcaggg
    20281 gctgaaggag catgaaggca gggagcatct ccatgaccat catgtgcatg gagcggaggg
    20341 ggaagcacca aaccggggat agtcccacat cgccggcagg ggagtttttt acctacggtg
    20401 caaagatcgt aaagcaaaaa agccctcgcg tgtgagggct tttttgctat caaggaggag
    20461 gtggtacggg tggtgctaaa aaaactgtgc aaggatctcc ttgcgcgggg agggaatgac
    20521 gatcttttcc accagcagtc cgcggcgttt gaccacggcc gcgccggcgt cgacggcgga
    20581 ctgcacagcg gcgacgtctc ccgtcagggt gacataggcc ttgccgccga tggccatggc
    20641 gatatggatt tcgagcagat cgacggcggc ggccttgacc gcagcatcgg cggcctcgat
    20701 ggcggaggcg accgaaaagg attcgatgat gccgagggcg cgcagttccg gggtatggga
    20761 aatgccgctc agggcgggga agacgctgcg gtggacattg gggatgacaa aatggtcgac
    20821 cacctgatgg ccgcccagct ccactccggt ctcgatgctc gcgttgacgt catcgacatc
    20881 gccgttgacc aggaccatat atttgcccgg gcagatggtg cgattgacca ccacctcgac
    20941 cagggcccgc ttgaccatcg catctgccac ctcgatgcct ttggcgatgc ttgagagttc
    21001 gaccagacct attgcagcag ccatgatact atcctgcctt gtaatttgtt aacccttgat
    21061 gacgatactt ttttcggata cggcggcgac gatgccggag atggaggcgt gcaccgggca
    21121 gcccagtttt tcctcggcca cctcggcgat gacctgcccc ttgacgaccc ggtccccgct
    21181 tttgatcaca gggaccgcgg gggccccaat gtgctgctgc aggggcagct cgacctcggc
    21241 cggtgcaaag ttcagctccc tgaagtgggc cttgcggtcg tagggcttga tgcccaggcg
    21301 ctgatagagc atggtgatcg gaatttcacg gctgctgcgc acgggatgga catcgagaaa
    21361 gagctcctta agctcttcag tggtgcgcga gatctgtttc tcgcgcagca gctttttggc
    21421 gtcgacgcag atgttcttgg gatcgagctt ctccgggcag ctgaagagcg aacagatatt
    21481 gcattcgcag cagtaggccg cccagagtga gtagcgctcc ttctcgctgc cggtcatgag
    21541 cagcgagcgc atcaccttgt gcggctggat cggatagccg agcaggtagc gcgggcagag
    21601 ctcagtgcag agcgtgcact gatcgcagca gctgtggccg atgcgttgat actgggcggg
    21661 cggggtaagt ttgcgcactg ccaccggatg gtccgcgggc agaaaaatga ggccgccgtt
    21721 ggtcttgacg acgggcaggg cgagatcgtt ctcgactccg cccatcatga tgccgccggt
    21781 gaagaccacc gggcggttga gggtagtgcc gccggcgaga gcgaggaggt cgctgaagcg
    21841 ggtgccgacc gggatgcgca cggtcatggc ttcgccgacg gccccggtga tggtgacata
    21901 tttatccgtt accgcatctc cgcgcacggc gcgggcgatg ttgacaatgg tttcgacgtt
    21961 gttgacgatg gcgccgacat gcaggggcag gccgccgggg ggaatctgcc ggccggtgat
    22021 ctcgtagacc agcacatact cgtcgccggc gggatagaca tcctccatga tgaagaggtc
    22081 aaagccgagt ttttcggcat cgggccggag gagggcggcg acgtcgctgt tcttgcgctt
    22141 gacagctatg gtcaccctgc gggctccggt gagctgctgc atgatcgaca gcccttccag
    22201 gagaagctcc ttttcctgga gcatgacctc gcgatccttg tacatcatcg gttcgcattc
    22261 ggcgccgttg gcgatgatct gttcgacgcg ggcgtccagt tttttatagg tggggaatcc
    22321 cgcccctcct gcgccgacaa cccctgcggc gcgtattttc cggaggatgg tgctgttatc
    22381 ttccatcaga atgctcaaag gtttgattca tggtgatggc caggggagtc aaatagaggg
    22441 gttccgaggg cagaatgacc tccaggcccg gcaattcggc ggcgaagata tcggctgcgc
    22501 ccgggaagca gcaggtgccg ccgctgagat agagttgttc gaccggacgg tcggcgagat
    22561 gggttctgat gatatccgcc atcttttcaa agaccggctg gatgatgggc agaatctgcg
    22621 cagcgtctgg gccgcgcttg atcgcttccg cttcttcata ggagatgcgt cgattgccgg
    22681 ccagggtgag ggtgacatgc cggccgccgg tgggctcgtc ggcggaatag acgatccgtc
    22741 ccccttcgac gacggagatg ccggtggtgc cgccgccgat gtcgacgacg gcgccgcggt
    22801 cgatctcgag cagccgggcc accaccgagg gttcgtcgag catcccggtc acattcagat
    22861 tggccgcctt cagcacgttg acgcaggcat agggatcagt gcccggaggg aaagaagtgg
    22921 aggcggtggt gaagtgctga ccgagtttct cctccgcttt ggcgaccagg gacttgacga
    22981 tctgaatggc cccgaaatag tcgagcacca ccccgtcgcg aacaacatcg gcccattcga
    23041 gaaaggcggc atggggctcg ccgctctcgt cgcaggcgat gaggacgacg tcggcggtgc
    23101 cgaggtcgat gcccaggtgg cagggagggg ctccggtcac ccgccgcttg ttgttgacca
    23161 gggcggcgac gcgccgcagg cgttgattca ccctatccag tgcggacatg gtcatcctca
    23221 gacaatgcgg aaatggtcga cgatgacgca gcggcgcagg cggacgaagg tgcggctgca
    23281 ggtaactcct tcgccggtgg gggtggagat ggtcatcgag gtccagccct cccctccgaa
    23341 gccaaggccg gcgatgcacg ggccgttctt gacgaagatg ctggtgttga tctcgcgcga
    23401 catgcggtgc agatggtcga tgttgcgcga atgcatcgcg gcggtatggt gcagcccgtg
    23461 ttcgagctcg atggcgagat cgatggcggc gtcggcgttg gcggcgcgga tgagggaaat
    23521 gaccggcatc atcagttcga tctgggcgaa gggatgggac ttgtcggttt cggtgaaaag
    23581 caggcgcgta tccgggggca cctggatgcc cgcctcgcgg gcgatgacgg tggcgtcccg
    23641 gccgaccatc ttgcggttga tgaccggatt gggcccggga tactccttca ggatgacctg
    23701 ggctacggcc tcggcctggc gggcgtcaag ctcgactgcg cggttcttcg ccatttcgcg
    23761 tttgagctga tcggcgacgc tgtcgacgac gatgatttcc ttctcgtcgg cgcagatgat
    23821 gttgttgtcg aaagaggcgc cggaaacgat gcaggcggca gcgtggggga tatccgcgct
    23881 ctcgtcgacg accaccgggg gattgccggc gccagcggcg atgagacgct tgtcggtctg
    23941 gcgcctcgct tcatggacca cggcctcgcc gcccgtgacg acaagcagac cgatgcccgg
    24001 gtatttgaag agctgctgag cggtctcgat ggtgggctgg gccacggcgg tgagcaggtt
    24061 gtggggtccg ccggcagcca ccaccgcttc attgagcagg ctgatggtgc gctgcgagac
    24121 ccgtttggcc gccggatgag gcgcaaagac caccgagttg ccggcactga ccatgctgat
    24181 cgcattgttg atcaccgtgg ccgccggatt agtcgaaggg gtcactgagg cgatgacgcc
    24241 ccagggggcg ttctcgatca gggtgaggcc gtgatcaccc gagatcgcct cggcgtggat
    24301 cacctcaggg ccgggggtcc tgagggcctg cgaggtgttc ttcgccacct tgtcctcgac
    24361 cctgcccatg ccggtctcct cgaccgccag ttcggccagt tcacgcgcgt ggcgtgcgcc
    24421 ggcttcacgc atggcgagaa taatcttgtc cctgaattcc agattgccga ttctcttctg
    24481 cgcggcggtt gcggcggcga ccgcttcgtc gagggtcgcg aagataccgg cgggcagcgc
    24541 ggatctgccg gtggtatcac ccgacgttgt ggtgcgcatg cgttctacca ccgcctcgac
    24601 gatgcgggcg acctcttgtt gactcaaatc cattacgggt tctccctctt atcaggccga
    24661 ttcacttttc tgatagagca ccccggcctc cgaacttacg gtatcgatga tgccgacgat
    24721 ggtgagatcg atcggcgtag cgttgttgat ggaaacgcgg gcggagctgc cgcgcaccac
    24781 caggacccac tccccctcgc cggctccgat ggtgtcgagg gcgacctggg ttttacccgc
    24841 cgccttgccg tgttcatcga taaaccggac gagcagcagt ttgccaatgg ccagttgctc
    24901 cattttgacg gtggagacca cctggccgac cacttttccg agttccatag gtacctctgc
    24961 aggtgcctgc gaggcgcttt acgtccccgg ccggacggcc agggacgggc acgcctgcag
    25021 attacttgat ctcgcctttc atcttgatcg ggaagatatc ttccagatcg ccatgggggc
    25081 gcggaatgac atggaccgaa atcagctcgc cgattttctg ggcggcggct gcgccggcat
    25141 ccgtggctgc tttacaagcc gcaacatcgc cgcgcacaag ggcggttaca taaccggatc
    25201 cgatctgatg gaatccgacc agcttgaccc gcgccgcttt gaccatcgca tccgaggcct
    25261 cgatgagcgc caccagacca cgggtttcaa tcatacccaa tgcttccata ccactttctc
    25321 ctttccttgg aaaaattacc cttcaatagt gcctttcatc ttgatcggga agatatcttc
    25381 cagatcgcca tgaggacgcg ggataacatg tacggagatc agttcgccga ttttctgcgc
    25441 cgctgcggcg ccggcgtcag tggccgcctt acaggccgcg acatcgccgc gcaccaaggc
    25501 ggtcacataa ccggacccga tctggtggaa ccccaccagt ttcactctcg ccgccttgac
    25561 catagcatcc gaggcttcga tcagcgcgac aagtcctctg gtttcgatca ttcccagtgc
    25621 ttccatgagt tactcctcgt tagttattgg tatgttgaaa gagatcaact tctcgccata
    25681 taagcggctg tcatggagat ttcgacgata tcctcccaac tgcagccgcg ggagaggtca
    25741 tgcatgggtt tgcacagccc ctgcaggaag gggccgagcg ccttgaatcc gcccagacgt
    25801 tccaccagct tgtagccgat attgcctgcg ttgagatcgg ggaagatgaa gacattggcc
    25861 tggcccgcca ccgggctccc cggggccttt ttggcggcga ccgccggcac aaaggccgca
    25921 tcgaactgga gttcgccgtc caccttgaga ttgggggcct tggccttaac cagctccgtc
    25981 gcctgccgca tcttgtcgac cttggcgtgg ctggcgctcc ccttggagga gaaggagagc
    26041 atcgcgaccc tgggttcgtt ggcggtcagc cgttcatact gcgcggccgt agccacagcg
    26101 atgtcggcca gctgctcggg ggtcggatcg gggagcaccg ccgcatcggc aaaggcgtag
    26161 accgactcgc cgtcggggga gatcatcgca aaaaagctcg agaccgtgcg caggccgggg
    26221 gccacgccaa ggacgtgaat agccgcgcgc agcacattgg ccgtcgacga caggttgccg
    26281 gcgatgcaga gatccgcctg ggcgtcgcgg agcagcatgg cccccatgta aaggggatca
    26341 tcgaggcgct ggccggcctc ataggcggtc atgcccttct ccttgcgcga ctcgtgcagg
    26401 atgcgcacat aacgttcgcg ctccggcaca tgctggggat gaaggatgac cagaccgcgg
    26461 gtcgggatgt gcaccttgtc cgcgaattcc cgcagttcga agcggccgcc gaggaggatg
    26521 ggctgggcga ggcgttcatc cttgaggtag cgggccgctc gcagcacccg ttcatcgcgg
    26581 gcgtcgggaa agacaatgcg gcgcggctgc cagcggcagt attcggtggc gagatggatc
    26641 agcttcatcg ttcgagcacc actttttcga gagtgagctg tttgccctgt tccgccagca
    26701 gggtcagcag catgagtaca tagagggcgc tgctgaggcg gttggccgcc tggatcagat
    26761 cgggacgggt gacggtgagg tccggggcga tgaagaccct ggcagcctcg acctccacct
    26821 cacgcacctg ggcgcgcagc agattaagca gcgccacatt acggccgtgg ctcatctccg
    26881 ggacgatgtg gtcgtggccc agatacttga gggggttgtg cgagaccgca tggaccacat
    26941 tttcatcggc gccgccgagg gcgatcggga tcatcgtctc cccgctcacc tcgcttttaa
    27001 gcagattgcc gaggtaggag cggacgtccg cgagataggg gtggagccat gggaacttgt
    27061 tctcgcgatc gaattcgatc tggaccagga ccgtgtgagc gatcagggtg tcgatcttgc
    27121 cgcgcagggc gatgcggggg tggttcttga ccaccatggt gttggcatcc agatgggtga
    27181 gggcttcagg tttcttcgcc accggctgat ggcagagcag acactccgcc ttctggcctc
    27241 cggtgctgct ggtcagagga tgtacctgca cgggagcctc ctcgccgctt ttgtccggat
    27301 tgaagacccg gccttcggcg tcgatgtatt tgatgacgat cttgcgttct tcgagcagct
    27361 gatgcgcggc cggggtcagt cggctgtcga gggggagatg gacctcggtg ccccagccca
    27421 gaccgaattt ttcgcgcagc cagccctcgg tgatatattc ggaagccata cgcttatccc
    27481 tgtttttgcg acgcccagtc cgccggatag gtcacgtaca tgatcttggt ataggtgggc
    27541 gtgccgaaga tgatctggct gcccttgggg atgtaaaata gatcccccac cttgccctcg
    27601 aagcatttgc cgttgatggt gatggccagg gtgccctgga ggacgatgtc gatctcgtcg
    27661 taattgaggg tccaggcgaa ggagtccatc ttgcgccagg acatgaatcc ggcgcacatg
    27721 ggcgaacggt ctttcgaggt gatgacgtcg gcgaggcgca cctgtttacc gggcccggcc
    27781 tctggcatga tatccatacg caccgagccg ccgcgcacca cgatcaggcc gctgtcgtcg
    27841 accacgcgct ggcagcaggg ggcagtgggg gcgagggccg cggtgatgga gggctggctc
    27901 acgggcaggg gtgaaggcga cggcggacag gctgcgggcg gctgctgcag ctgttccgcg
    27961 accagccggg cgatcgtggc ggggtcgcgt ttgtccgcgg gcagccgctg gatgacctgg
    28021 gcggcgatgg ctttgaccag ctcctggtcg ccgccggcgg cctgggccct ctgctgcagg
    28081 gggcagctct cgcttcccgg ctcgacgaag cagatgccga acttggcggc cagggtggct
    28141 gcttcggggg tgatgatggt cgtctgcacc gaggccggca gctccttgat gccagcggtg
    28201 caggcttctt ttatggtctg ggctgtgatc agttttttca ttttggtttc ctttttactc
    28261 tcccggatgg acggcagaaa agggctccct gctcagaaaa cggcgcaagg catcgatgct
    28321 ttcgggctgg tagaccgaga ccggatagat ggggccctgg atgcccgcat cccggatgag
    28381 ctcctcgacc tcggagaccc gggcatcctc aagatcgatt ttggaaatga cgccgatgat
    28441 ttgtttggtc tcatagatat gaaacaagcc aaaaggcagt ttgcgctcca gggagttggc
    28501 ctggtgcaca tagacaatgg tctggatatc ggtcatggtc gagatgagcg ccgtatagag
    28561 ccggggattg tcaaagtact cgcccggggt gtcgatggtg cactcatcgt gaaagaccag
    28621 ggcctgggtt ttctggacct cccccagccg gccgagcagg gctgcaaaga gagtcgactt
    28681 gcccaccccg gtgccgccga tgaggataaa attgttgagc atcaggtcac cgtcaccgcg
    28741 cagggggtgt agttgagaat ctcctgcaag gtcttgttga ccgatttgag tgcctcctcg
    28801 acagcaccga cctcgccatg gatcaccagg gcgccggtga agcggtcgag aaacccgatc
    28861 tggacgttgg cggccttgac ggcgatatcg ccggcgatca tggcggtctc gcccggggtg
    28921 agggtcataa tgccgatggc attgacgtag gggatgccga tttttttgca cacctcctca
    28981 cccggatggg cgatcatatg ggccagggtc acctgcttgc ctggcacata ctcctggatg
    29041 atgcgctctt tgtcctgata ggggtttctc gaatcttcca tttcagtcct gcattctggc
    29101 caacagggcc ctggtcagct cgccgtattc gagcgcgccg cgggcggagg gggcatagcg
    29161 gaggatcggt tccccggcgc gggccgcttc cgccagtttg atattcatgc cgatcacggt
    29221 cggcagcagc agatcgccgt agcgctgctt gagggtgtcg tagacttcgg ccgcgctcct
    29281 ggtacggcgg tcgaagtggg tcacaaaata gccgagggtg cgcaggcgcg gattgagatg
    29341 ccgccgcgct ttggagagca tgtcgtcggt cacctcgagg ccggcgacgg ccaggtactg
    29401 ggcctggacc gggatgaggg catagtcggc gatggcgagg gcgttcatcg ccaggggccc
    29461 catattgggc ggacagtcgc agatgaccag gtcgaactcc tgagggatct gctcccggct
    29521 gatgaaaaag gcctccgccg catcctcggc gacctcaaga taaaagtcgg cgagggaggg
    29581 ttcggcgggc aggcagaaga gctgctcatc cccggccgga tggatagccg cggagagatt
    29641 gtggcttgcg tcgacgagat agtcgcagag ggtcttgacc cgtccggcta cggggccgaa
    29701 gccgaggtgg cgcgatgctc cggccgaggg atcgaaatca gcgagcaggg tcttgtggcc
    29761 gaagcgggcg caccaggcgg caatgttgac cgcactggtg gttttgccca cgccgccctt
    29821 gttgttgatg acgacgacga tcttcatttt ttatccgtga gcggataata cttgcgctgc
    29881 attttttcga tgacaccctc atccagcagg gtcttgagga cgccaaggat ggcggggcgg
    29941 tcacacttca aggccgcggc cagttcatcc gcggtgctgc ctgcggggcg ggcctggata
    30001 tggcgcaggg cgcgactgct caggtccgct ccggtgcgtt tgggtttttc aggttgcggg
    30061 gttttagc
//
LOCUS       DAOPWU010000005        29602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_1505_length_29602_cov_5.575625, whole genome shotgun sequence.
ACCESSION   DAOPWU010000005 DAOPWU010000000
VERSION     DAOPWU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 29602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29602
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1505_length_29602_cov_5.575625"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..920)
                     /locus_tag="PKM23_00795"
     CDS             complement(87..920)
                     /locus_tag="PKM23_00795"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNY90035.1"
                     /translation="MERRVSFGLSHGLTPMVRNLLIINAAVYALQLPWAHQGTASPLL
                     HWFALWPPRVLHQFALWQILTYAFLHGGIIHILFNLFTLWIFGCEVERVLGGRKFLFI
                     YLISAAGAGLCHLLFNTHSPVPVIGASGAIYAVLVAFAVLFPDRVITLLLFFVLPVQL
                     KAKHLVALFIAISLFSGIESNIFGNRDGVAHLAHLGGALTGFLLLRGGPMLDSLLFET
                     RKRREWRHKAEQNRLQARQKAERDEIDRLLDRINEVGYEALSREEKKTLLKNSKKIKD
                     G"
     gene            complement(982..1653)
                     /locus_tag="PKM23_00800"
     CDS             complement(982..1653)
                     /locus_tag="PKM23_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90036.1"
                     /translation="MSSKNLYTCAVIALGLLIAAPSHAQRRSGPSWSAPKGLIGIHAG
                     YLNPKDPEGGMLAGCRLGTMVDESVSIGIGFDLWHKIYRDEKEVADETRPDRNTKTYM
                     TELEISDWAMPLTLEIAAKVPLARYMGYTVRGNIGYTFLWSKYSNYLENSSTNRRFGG
                     ICWQLGAGLYSEIGSRSTLVADLFYNSSEASSEVTKSKAGLPIFERVDLAGFGLRIGV
                     ILDMR"
     gene            complement(1774..2241)
                     /locus_tag="PKM23_00805"
     CDS             complement(1774..2241)
                     /locus_tag="PKM23_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNY90037.1"
                     /translation="MEKHEAYKWAAKRVRAQVKRQVDPIATLANTAAILKEYFPSFFW
                     VGFYFVREEYLLLGPFQGPPACVKLARNQGVCAASAERQQTILVPDVHDFPGHVACDA
                     RSRSEIVVPVHDEMGTLRAVLDVDSESLAAFDEQDAAGLQTVAGLLRGIWTTT"
     gene            complement(2246..2746)
                     /locus_tag="PKM23_00810"
     CDS             complement(2246..2746)
                     /locus_tag="PKM23_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNY90038.1"
                     /translation="MAKIVGRVWKYGDDVNTDVIFPGKYTYTVTDPAEMARIAMEDLD
                     ADFAKNVQPDDIVVAGKNFGCGSSREQAAFCLKYAGVGAVVARSFSRLYFRNCINAGL
                     PALTLPESADIFVAGERIEIDLSAGTIQCQAGLFHFPPLPEAVIGIFNDGGLIPHTRK
                     ALGIDS"
     gene            complement(2759..4012)
                     /locus_tag="PKM23_00815"
     CDS             complement(2759..4012)
                     /locus_tag="PKM23_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNY90039.1"
                     /translation="MGKTFAEKVLGQKAGKNVVPGEIVEVKPDIAMSHDNTAAISKTF
                     KEIGVAKIDDPNRHVIVLDHCVPAANEKFAQNHKDIRAFVAEQGIPNFFDIDRGICHQ
                     VLPEEGFALPGYLMVGSDSHSTTYGAFGALATGIGRSEMAVIFATGKIWLRVPETMKI
                     IVKGRLPKWISSKDVMLKIMGDLSADGGLYKSVWFTGPAIAEMSVSSRMVLTNMAVEF
                     GAKNGYMEPDDQTLAFLKGRARAPFTVVTSDPDAELESVHEFDVSDLEPLVACPHSVD
                     NVKPVSAVKGTRIDQVFFGSCTNARIEDFEIVAGVMKGKRVHPSCRMVVIPASRQVFL
                     EALAKGYIQILGEAGATIVNSGCGPCMGNHEGILAPGERVLSTSNRNFKGRFGCKDSE
                     IYLCSPLTAAASALTGEISDFREHL"
     gene            complement(4178..6919)
                     /locus_tag="PKM23_00820"
     CDS             complement(4178..6919)
                     /locus_tag="PKM23_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNY90040.1"
                     /translation="MKYAQKSFALVATAALLLLGAASRPAAQDAGGSPVSTMEKGSIS
                     GRISGLDSSLIGIASVTAWSNDPSIMEWGKGRAVVDSLFSFRIDSLAPGGYYVVAEAP
                     GYLPQYYNGVAYWSAATLVTVYPGQVTGGLTFELKPGTLVHGGTISGQVTGSDGLPLP
                     FVNIIASTLFMLDPEKRDFIDIGYASSDENGRYTMVNLPPDDYYLRAEYWNTWFGQTL
                     WYPQADSQADASTVTVSENSETSGIDFTFQVPSKAGRIAGRVVDALGNPLAAAGIQVM
                     AAPDQNIRWNWIWLYADTDADGYYQIDQVPHGSYIAYCWAQNGWDYIQLWWPDATTME
                     SAKIITLSDETPSWQADFTLPLTPGTAALGGRVVNTEGRPLANAYIQITTAGNEYDSA
                     AGRSFYAWASTDSSGLFKVDRLSPGSYIACATCWEGDSFGQGWYKDAGSLASATPIVL
                     TEGENRTDIDFALHVHPIYGAIVGTVTDAATGQPLPRAYVQVDYRQDSADYAIRRFAW
                     WPYYQITDDQGAFVFESLPEGSYKLSVYTNGAFAWYPDALVEEMAAPVDVVGGRKSEA
                     NFALTLRQSGTATISGQVRAEHGFVATEGDARPGNVRLEAVLDEYIPEIAVVMAKPAV
                     TIMLWPDSEQFYTAVTDPDGSYTLKGLPDGEYYLSSFAPGHMLQYYKETFDPAEAVLV
                     KVEAGQVITGIDFTLRANPWYFMKEGDAAGRNALNAIISGTVNDENGQGIAGAAVYLL
                     DGSGQPVTWATADENGRFEIMGIANGPYYLQAGKLGYATTFNGNASSREATTPLIAAN
                     GLTEVNITLPPARSTGVDVRVLPDKVALLGNYPNPFNPETSIHFSLPSPMQVTLAIYD
                     RLGRRVRQLHTGALPEGEHRLHWDGRSEQGETMTSGIYFYRLSTPAGIRTGKMVLMK"
     gene            complement(7015..8253)
                     /locus_tag="PKM23_00825"
     CDS             complement(7015..8253)
                     /locus_tag="PKM23_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNY90041.1"
                     /translation="MKRPWLVLPLALLFILSGCSRDASPTDPRDNPPARPLTAHESAL
                     ARDSNEFGWRLVQRMALEEGQKNSFISPLSVTLALAMAYNGAGGETEQAMRTTLGFPD
                     LSRDELNAALSGLSSLLMGLDPSVQFRIANAIWYRQGLQVKPDFTERNRLAYNATIAP
                     LDFNSPQAPSIINDWASNKTNGRIPRVIDTIDPDLMMLLLNAIYFKGDWTTEFDPSET
                     RPDRFFLADSSQASCQMMQLTDNLPYFENELFQAVDLPYGDGQFAMALLLPRTTLEDL
                     TSRLDNAAWDEWHSSFSRQRGTLQLPKFKLSCDVTMNNALKAMGMAVAFDDHRADFSG
                     ISTMMPLFISYVKHSSFVQVDEEGTEAAAVTVVGVGTTSIGGPGGFFMRIDRPFLVVI
                     HDHHSRTALFVGRIYQPEWN"
     gene            8535..9419
                     /locus_tag="PKM23_00830"
     CDS             8535..9419
                     /locus_tag="PKM23_00830"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HNY90042.1"
                     /translation="MSDRLPVFTAVLGLAVLLTCLQASDSPLYHLAPLPVIDQDIRQT
                     TARGLTNAEKMVYYSGRFMGAPYLLVCEGEGENGRYETQPLLNLQKINCMTYCEIVMA
                     LTLSRNYEEMFNVLQHIRYRDGYISMATRNHYTMVDWLPANSWCLEEVTRQVGGADVV
                     ESTRTIRHGLFFQGKGIDDLPAVLPDRTVCAAYIPLQKLALHEQALNSGDVVALIQDK
                     PDIFSAHMLMVIKREGRTWFRHASMSAGKVVDEPFAEYIAKISKRPLYQGMSFMRIRE
                     QVDWSLPGAARGKNPLHE"
     gene            9441..10451
                     /locus_tag="PKM23_00835"
     CDS             9441..10451
                     /locus_tag="PKM23_00835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNY90043.1"
                     /translation="MGGVKMGVGVLVTVCAVVLGVVGCGDHELSATTQSAAALWELSE
                     RAVFKEGRPANTFVATLLESLAATSGQGKPVSEGEFRELLGRLSPEVYPENIIKYATP
                     QSVTIQNHEHTDYRKVFLKPRRIAEGARFIARHRAELDSAQARYRVEVRDIVSILMWE
                     SGLGEFVGSHFIVNVFLGQILYLDQARDLAVRELIIKGEADTTALKLGEAEKKRLDRL
                     RRSAALNLAALLRMSKEKGVEAAELRGSWGGAIGYVQFMPSSMQFARDGDSDGRIDLC
                     TWPDAIHSVANYLHANGYGASGRGRRRAIHAYNPIDSYVNGVIEYADAVWKKYQEDTL
                     QR"
     gene            10466..11179
                     /locus_tag="PKM23_00840"
     CDS             10466..11179
                     /locus_tag="PKM23_00840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNY90044.1"
                     /translation="MARRRHYTRFKFEGTFTRGYDLFNHVATLGLDLFWRRKAAEQVS
                     RDLNGTACPGAVLDLACGTGDMAAAVARAHPGVQVVGSDPSAAMLHYGREKFETWNGR
                     IAVVQAVSLLPFPDGVFSAITCAFGVRNFVALADDLQESLRLLRPGGRLNVLEFFIPG
                     NLLIKAVLKSYNALIFPFLGFVLTGRVGPYRYLFNSIFTFRTVPDFCTLLEGAGFRDI
                     RIRSFFFGMVHLVSGGKPQ"
     gene            11213..12130
                     /locus_tag="PKM23_00845"
     CDS             11213..12130
                     /locus_tag="PKM23_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNY90045.1"
                     /translation="MATLKAYFLATRPWSYTVSVLPPVFGLLIAIIDVAGLQVDWVKF
                     VLTLLGCNLAHSGSNTLSDYFDFKTRVDREGTYGSSGMLVAKVMTPPEMLRWALILYA
                     AAGAIGLYLILSSGNPGLLIALVAAGFILGFFYTMAPFNFKYHALGDVGVFLAFGLLI
                     PLGSYAVQAGLFSWKPLLWCIPAALLVDAILHSNNLRDIPFDSAVHIRTVPILIGERR
                     AQTMYYVLVLGAYALIPLLVIGTGLTWLSLLVFLSLPVALQNIRTVRRKQEMAIEQFA
                     GIDAATAKLHLLFGLLFIAALAGRIFFLP"
     gene            12139..12771
                     /locus_tag="PKM23_00850"
     CDS             12139..12771
                     /locus_tag="PKM23_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNY90046.1"
                     /translation="MHKRILYTAIALAFVLWYIMFVLRPFNFWAMMAFSTSLLSLVVL
                     WIGKPFADRGEWSWSNLWLGVTSALLLYAIFWAGNQGLILMERVVPGLFADRPQNLAA
                     IYGNRGELAPHWVALLLFFPVGFGEEFFWRGLVQKHFAGTLGRWRGFLIATFLYTAVH
                     FATGNYVLLLAALTCGLFWGALYAWRGHLFPVLLSHMLWDPLIFVLFPIQ"
     gene            12783..14027
                     /locus_tag="PKM23_00855"
     CDS             12783..14027
                     /locus_tag="PKM23_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90047.1"
                     /translation="MRDGENGGKCRGGERRGRCSEPLLSLLILLLLLPWNRGEAQPAS
                     SQLPEPILLHRLQEDIADERLDEFTLIQAAFILSGAATEDSLASCLSWYEEVLSRLRA
                     LYLDPFKRLESASTIFSYLHSFYLREYKEEATTLLDVVHRQKFNCVAGTILYNLVCAD
                     LGYATEAFETPTHVYTIFPDFTRTVTVENTSPMGFNIMRNLQAYSAYLLNYYPEHRAA
                     QIGLDRIYAYENSRGRRINNTELLGLLAYNRAYFARERNDFAAAYEFVLLAQEFNRDS
                     RSNIPFEQSLYFQWGKTLYEAGSFDDAFAVFADGAYRYPEMKELAQNSRAAYFQALRR
                     HGQQLNWPESRRLVMEMTELALLGPAEMEQQQEILSGWAEYFYRRAERRPLLEVIELM
                     QSAHPEEPRLQEMRRVAERLPE"
     gene            14062..15204
                     /locus_tag="PKM23_00860"
     CDS             14062..15204
                     /locus_tag="PKM23_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90048.1"
                     /translation="MPIYDLEVFLRLSADLNYLLVVKPTNWSSVLDKILNEQHLPVAK
                     QEILLETMEYLDQVYGRKRRRMGAQAIIHPLRATALLVRSMPVPLHLDMMASLLHDRF
                     EDFPSVSRPADFGESIDPPLRAIMNKLPEDEQWFLTERLYWLTKRSGENYYRYIGRML
                     SEACRTPEAVRVKLADRLDNTLDMRMDFRHANDNIDFFEELFQMMFLPSYHPNKSNFA
                     HPSHSAMDGSERLYQLYKNIVLISLVRQKKAAEDDAISITLFKILIQASIKEAKRIAQ
                     HIFSYHQTNITRMRELVIETMQYIMEGGIDAVTAPTLGKMLDGLFLKWFNEPDRRVRT
                     RNLAELYKDKDLMVEASIAFIVIFYRFLDDPAYFVHGISEEGIKVE"
     gene            15323..17374
                     /locus_tag="PKM23_00865"
     CDS             15323..17374
                     /locus_tag="PKM23_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90049.1"
                     /translation="MLKTMQEKAGGLVLLLAGIGMMISMASNAAAGVPQSAVERTIAL
                     LQSRHEAGVHQRIELGVRQAASLWREEDGTESEFSDFCAAHFVADPQQRAALLDRFES
                     NLETLYGHAHEVSRAWSIPLQLELGPVLPVDYLFAEYSPFAHIADDLFHNKIAFSALL
                     NFPFYTLEERLRLGPSWSREEWAEMRLAGQFSARVPAEVLQASSVAYVQADDYISNYN
                     IYMHALVDEQGRRLFPEGLKLISHWGLRDELKGQYAEKEGLARQRMISKVMERIIRQE
                     IPQAVINAPVPLWAPYTNLAGEEGRMAPAAPEEDVRYSHLLAIFAAECGVDPYYPGQP
                     TKIARRFDREREIPEAEFETLLIRLLTDPIAPRVARLISRRLQRPLEPFDIWYNGFKP
                     KSALGESELDRIVSAKYPTTAAFQQGLPEILTRLGFNPETAAYLESKITVDPSRGAGH
                     AMGAMRREDNAHLRTRIPEGGMRYKGYNIAVHELGHCVEQVFSLNRIDHTLLAGVPNT
                     AFTEAFAFVFQSRDLELLGLAAPDAQAAHLNALDTYWGACEIAAVGLVDIRVWNWMYE
                     HPDATPAELKAAVVEIARQVWNSYFAPLFGVRDVILLAIYSHMIDSGLYLPDYALGHI
                     ISFQIERYLQDKNLGTEMERMCRLGMLTPDGWMSAAVGSPVSVEPLLEAVREAVKVIK
                     K"
     gene            17595..20036
                     /locus_tag="PKM23_00870"
     CDS             17595..20036
                     /locus_tag="PKM23_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90050.1"
                     /translation="MQDSLARRNAMPLDGVWHLQISGGDRRSLEMPAALRLREQCTLE
                     KKFALDSTMISSSYELAFDGLDGLSTIYLNKKIIATHSAGSAPFAIQVGKQDLFLNEE
                     NELIIQLDGRLDYRRSLPLLVRNRGIPLSGNGLFRPLVLRSGKTPFITSLSLNPAESS
                     GMGLQTLDLRAVVALGGMDSLAMASLASMRGQVEILDGHSLQSLFVSPQLPLNATAVD
                     TTSLGVTAVIPAFRHWAPGAPQRYRIVMQLFLGSEVIDRASVWFARSQPGQWLAAAGE
                     KGGGFRYRAVDWVEDERQILLPQQEQKSVILEDLRGIVDLGANTVRLPGGLPGEFFLQ
                     SCDSLGLAVLVEIPVTHIPSAHLNNAAIRQKARSALTDMIRTCRSHPCVAAWGLGSGY
                     DPSDLRAQAFVRDLAAIARELDDRPVYASIRGKKLAAHALPVDLQIVEVPLEKTSTFA
                     QGAWRTNGPYLLQLSSPLDLRDSSDRSAQQNQAYYLKTAILDAQRRSQGAGLLISPWK
                     DWRGEAPHTYWGPRQETRLFVAGLLDEKGQQRLACQVVKAAFKNSEMPELLPADVPAE
                     DPPVFQIISIVLIVLLLFYIRTDKRMSHYLKRVFVYPHGFYMDLIENRQVNPFLTGVM
                     GLASFLTVSTLLASLIFFLRENSLFDELLTWFFPNSTAKNQAIALIWNPERMILLLTV
                     VMVGLALLQSILYKLIVLWQRRYLRFSQILTFSFWVPANFIFALPLAVVLFRALSRSN
                     LVTLSLVYLGIMLFWFMVRSLRGTKVILQTTTFRAFLVVAAGLFFILLAAGLYMEQTR
                     AMTAFASYYWSLLGQ"
     gene            20124..23708
                     /gene="smc"
                     /locus_tag="PKM23_00875"
     CDS             20124..23708
                     /gene="smc"
                     /locus_tag="PKM23_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNY90051.1"
                     /translation="MYLSELTIVGFKSFAKKTHVTMHDGITGIVGPNGCGKSNIVDAI
                     RWVMGEQKSGALRSEVMGNVIFNGTATAKPVGMAEVSLKIENSGNTLPIDYAEVIITR
                     RLFRSGESQYLINGNSCRLKDILDLFMDTGAGPHTYSVIELPQVERILNGKEDERRHI
                     FEEAAGITKYKLRRKATFRKLESTEKDLIRIEDIMSEVEKNVRSLRRQVAKAERYQEI
                     TSELRDLEIKLANNDYAMILDELEPAETRLELIRDEREGASSTLATQDADYETTRARL
                     LEIEKKLLENQRTVSDLDREVQKLEERILVNKERIRSLEESGLRSSQERERFDAHLVE
                     LQQQYNAAQELYLEAEKNLTARREEYEKANGELQEVRVQFESKRSLAREAENRVLRIT
                     EELSRKQTEGERLKATEESLSLRLRQIEADAEKDAARATELTEILNKTRAEEAALADT
                     LGKKKQRLTEISRREEEAKRLFETLQKSESQDRSRIEVLGHQAELVRRLIDNYEDYPA
                     GVRFLATLKKEGFSTHGPLANIIHVENQHRTAIAAALGEAATYLVVEESNTAFNGIGL
                     LREERRGIVTFAPRQEMGAILRERPLLSDLGVIGWADELVSCEDRYSALVRQVLGAFI
                     VTQDLQTAHRVFAELRGQSYSVVTLTGEVLSHNGYIRGGVLSKNQSDFVGRQEQLAAL
                     TGEIEKLQQGLEARRIQLGQRETEIQENKIEAEKLRKEIQDSEQALGSLRIQLGRQTF
                     EEQALAEAREKREGERQRIIGEVSQLGHNLELQNLGAGDLQTQRHESAERAAALSEEI
                     KAIEEAVDRATGRARELNVAMARMQSEFEAMRRESDGLHRQIVETEALIKMRREDAEN
                     ASAEIKELHQVNENYSQEVARQQVRRQEVQAVLDELEEQQYQANIRVAEAEKLIRASR
                     GKFDVLSESAHQIELRVSELKLKAENLQSRLWNEFEYKLQRMAVDVDINPNETHQRLE
                     NLRERIKEVGPVNLLALKEFEQEKERLDFLQTQRDDLIKARKNLMETIEIINTTARQK
                     FLETFEQVQRNFSEVFSKFFAGGRASLVLQETADPLESEIEILATPGGKKPAALTLLS
                     GGEKSLTAISLLFAIYLVKPSPFCIFDEVDAPLDDQNVQRFTHALREFSSNTQFIVVT
                     HNKLTMRAADQLYGVTMEEQGISKLVSVKFDSMKNDQLVPAASSAPAN"
     gene            23718..25157
                     /locus_tag="PKM23_00880"
     CDS             23718..25157
                     /locus_tag="PKM23_00880"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNY90052.1"
                     /translation="MRILAVIQKNVSFIALLLALAALPLPAQEEKSEERLRFNIDYTR
                     SRYSDSLSFCEFYALLPRKQLTYLPDGGSFKAEFKVTAEVFLQDSLVSSKIWKNVNHT
                     DSLSTIESGQNLFFLNHFILSEGDYEMSLLIEDLLKPESRGSYRFPLSITAFKGPELQ
                     ISDLQISSSIVRDTTRTSFLKNGFQVTPNPSGLYGLELPILYLYSEIYHLVPASSDSG
                     GQYSVAYKIFNADGRMVKEVAAKRRNKPGDSAVEVAAINVVTLVSGAYRVVAEVTDME
                     NGAVTSGMRKFFVYREGDYAEGGAAGQTKEEVPKTGSAGMDADRYAVLSEKELNEEFE
                     FTRYISTKEERKTFKKLNLDGKRAFMKEFWAQRDPSPGSPENEFKYDYLTRIQQANTS
                     YRGTFREGWRTDRGRVLLVYGPPDEVERYPFSNDNRAYEIWHYYSLQGGVQFYYVDKR
                     DTGDMELVHSTARGEIYDLDWERWKNPNE"
     gene            25217..26065
                     /locus_tag="PKM23_00885"
     CDS             25217..26065
                     /locus_tag="PKM23_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNY90053.1"
                     /translation="MKKLAQKCLGFIQLTRPVNVLIAGLSIFLAGYLCGIGDAGTKLL
                     AACLVGMLITAAANGINDYYDIEIDRINKPRRPFPSGRLRRGEGLVLVAISFAAALVL
                     AGRINPAALGLTLFSGFLLYWYSARLKRTVLWGNLAVSLVTALAFVFGGIAVGRFRYA
                     LVPAVFSFLMHFGREIIKDMEDVGGDAALNAGTFPVVHGLAPARRLATTVLLLLLPVT
                     WLPWFLGLYGRWYLLTVVAGVHSVLLLVIWSIWRRPGRTTYKWSSNLLKADMLVGLLA
                     IYAGRW"
     gene            26076..26528
                     /gene="dtd"
                     /locus_tag="PKM23_00890"
     CDS             26076..26528
                     /gene="dtd"
                     /locus_tag="PKM23_00890"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNY90054.1"
                     /translation="MRVLLQRVSRAQVSVGGETIGRIGPGLAILLGLKEDDTPELAER
                     MADKTAHLRIFADTEGKTNLSALDTGAEMLVVSQFTLYADCRRGRRPGFSYAARPEKA
                     EPLYEHFMAALHQLGFTVASGRFGAEMVVEICNEGPFTILLDSDEQAL"
     gene            26525..27136
                     /locus_tag="PKM23_00895"
     CDS             26525..27136
                     /locus_tag="PKM23_00895"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNY90055.1"
                     /translation="MSFSLESHLPHRTCYLASQSPRRKQLLQLIGLKFHVLPSGIDEE
                     ACTERDPVAHVQKLALAKAEDVGRRIESGLVVGADTIVVLDGEILGKPVDEEDAVRML
                     RRLSGRTHQVYTGFAILFQPEDDKVTGYEVTNVRFRPLQEWEIRSYVATGSPMDKAGA
                     YGIQDQSALFADRIEGCFYNVVGFPLTRFYLTLLNFIKEHAYE"
     gene            27129..28010
                     /locus_tag="PKM23_00900"
     CDS             27129..28010
                     /locus_tag="PKM23_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNY90056.1"
                     /translation="MNEVFKNLGEELARQRQEKELSIAQLAARTKITEAFFEKMEQGD
                     FAFLPPVYVRAFIRSVSTEIGLDPEVMMRRFSDEAGRLAAASSAQAAEAGVEPAAERP
                     AYKPRAQFEPTPDESGDKKRADMDWFKSPFTLGIMLIALLAALYFFFGRKPNVEPSAM
                     DSGIAVVEPVQESGSLRDVPLVPFTGDNRVDSTAVTQLSLTLRAEETAWVRIVYQDSL
                     VEEGVFTEGLSRSWLSPERFYLKIGNAGGIRLILDGQDLGLTGEKGQVVTIAVTKEGI
                     APLDPAQAPSRLTQMNP"
     gene            28024..29436
                     /gene="mpl"
                     /locus_tag="PKM23_00905"
     CDS             28024..29436
                     /gene="mpl"
                     /locus_tag="PKM23_00905"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNY90057.1"
                     /translation="MVLPEPKRIYFLAICGTAMASLAAMLKHKGYQVSGSDSGIYPPM
                     SDFLAEQEIEVFSGFDPAHLESAPDLVVVGNAISRGNAEIEEILDRRIPYISLPDALR
                     EFCIRGHRSVVVTGTHGKTTTTALIAWIFQSAGRDPSFLVGGIPNNFGRGFQSGSGEE
                     IILEGDEYDSAYFDKAAKFLRYMPEVGVINNIEFDHADIYNSVDEIIVAFRRFVNLIP
                     RSGLLVSCADYETVRGVSARAFCPVESFGFAADALWQPQAVRVTEKGLSFGIVHAGEL
                     LGEVEVALAGEHNVRNVLAAVAVARNAGIDFGSIRSALAAFTGIRRRLELKGSEGGVA
                     IYDDFGHHPTAIQETLQGFRQRHAGARIWALFEPRSATTRRSVFQKEMAEAFAKADAA
                     LIAPVDRPDKAPAGQLFSTEMLADDLRRRGREALAVSSVDEMVGYLLAHLRPGDVVIS
                     FSNGPFGGIHDRLLKGLKKK"
BASE COUNT         5762 a         8434 c         9223 g         6183 t
ORIGIN      
        1 cgccggagcg aggagaaggg cgatggttaa ccagcgcaga agatgctctc tcttgctccg
       61 atcagtcagc cgctgcatgc gaatagtcat ccatccttga tttttttgct gttcttgaga
      121 agggttttct tttcctcgcg agagagggcc tcatagccga cctcattgat acggtcgagg
      181 aggcggtcga tttcgtctcg ctctgctttt tggcgggcct gcaagcggtt ttgttcagcc
      241 ttgtgccgcc actccctgcg tttgcgcgtc tcgaagagca gggagtcgag catcgggccg
      301 ccacgcagga gcaggaatcc cgtgagggcc ccgccgagat gggcgaggtg cgccacacca
      361 tcccggttgc cgaaaatatt gctctcaatg ccggagaaga gcgagatggc gatgaacagg
      421 gccaccagat gcttcgcctt gagctggacc gggaggacaa aaaagaggag cagggtgatc
      481 acgcggtccg ggaagagcac ggcaaaggcg acgaggacgg cgtagatcgc tcctgatgcg
      541 ccgatcaccg gcaccgggct gtgggtgttg aagaggagat gacacaggcc cgccccggcc
      601 gcggagatca gataaatgaa taaaaatttc ctgccgccca gaacccgttc aacctcacat
      661 ccgaatatcc acagggtgaa aagattgaag aggatgtgga tgattccgcc atggagaaag
      721 gcgtaggtga ggatttgcca gagcgcgaac tggtggagta ccctcggcgg ccagagggcg
      781 aaccaatgga gcaggggcga cgccgttccc tggtgcgccc acgggagttg gagagcatag
      841 acagcggcat tgatgatgag caggttgcgg accatggggg tgaggccatg gctcaagccg
      901 aaagagactc ttctttccat acaccatgcg aggggtctaa ggttaagggg ccgcttcgcc
      961 cggacgctgc cgccgggagg ctcagcgcat gtcgagtatc acgccgatgc gcaggccgaa
     1021 gccggccaga tcgacacgtt caaagatcgg cagtccggct ttgcttttgg tgacctcgct
     1081 gctggcctcg ctgctgttgt aaaagagatc tgcgaccagg gtggagcgtg aaccgatttc
     1141 ggaatagagg ccggcgccga gctgccagca gattccgccg aagcggcggt tggtgctgga
     1201 attctctaaa taattgctgt atttgctcca gagaaaggta tagccgatat tgccgcgcac
     1261 ggtatagccc atatatctgg cgagcgggac cttggcggcg atttcgaggg tgaggggcat
     1321 cgcccagtcg gagatttcga gctcggtcat gtaggtcttg gtattgcggt ccggcctggt
     1381 ttcgtcggcc acctcttttt cgtcccggta gatcttgtgc cagaggtcga atccaatgcc
     1441 gatgctgacc gattcatcga ccatcgtgcc cagcctgcag cccgccagca ttccgccctc
     1501 aggatccttg ggattgagat agccggcatg gattccaatc agccctttgg gggccgacca
     1561 cgaggggccg gaacggcgct gtgcgtggga cggcgctgca atcagcagcc caagggcgat
     1621 gacggcacat gtgtaaaggt tcttgctgga catctgatta tcctcctagt cagacacaac
     1681 tgatgatggc cgaacctcag gattcgggaa atttccggtg gcggcgcgag cgcagccagc
     1741 gcgccggatc agcgcagcgt ggcagtgcct ctttcaggta gtggtccaga tcccgcgcag
     1801 gagtccggcg accgtctgca ggcctgcagc atcctgctcg tcgaatgcgg cgaggctttc
     1861 gctgtccaca tcgagtacgg cgcgcagggt gcccatctca tcgtggaccg ggacgacgat
     1921 ctcggacctg gagcgggcat cgcaggccac atgtccgggg aagtcgtgca catccgggac
     1981 caaaatggtc tgctgacgct ccgccgacgc cgcgcagacg ccctggttgc gtgcgagctt
     2041 gacgcaggct ggagggccct ggaagggacc gaggagcaga tactcctccc gtacgaaata
     2101 aaaacccacc cagaaaaagg agggaaagta ctccttgagg atggcggcgg tgttggccag
     2161 ggtggcgatg ggatcgacct ggcgtttgac ctgggcgcgc acccgtttgg ccgcccattt
     2221 ataggcttcg tgcttctcca tgggttcagg aatcgatgcc caatgccttg cgggtgtggg
     2281 ggatcagccc cccgtcattg aaaatgccaa tgaccgcctc cggaagagga ggaaaatgga
     2341 agagacccgc ctggcattgg atggtgcccg cgctgagatc gatctcgatc ctctcaccgg
     2401 cgacaaaaat gtcggccgat tccggcaggg tcagtgccgg caggccggca ttgatgcagt
     2461 tgcggaagta gagcctggag aaggagcggg ccacaaccgc gccgacgccg gcatatttga
     2521 gacaaaaagc agcctgttca cgtgaacttc cgcagccaaa gttcttgcct gcgacgacga
     2581 tatcgtcggg ctgaacattt ttagcgaaat ccgcgtcgag atcctccatg gcgatcctgg
     2641 ccatctccgc agggtcggtt acggtatagg tgtacttgcc cgggaagatg acgtcggtgt
     2701 tgacatcatc gccatatttc cagacccggc cgacaatctt tgccattact gctctcctct
     2761 atagatgctc gcgaaagtcg ctgatctcgc cggtgagggc gctggcagcg gcagtgaggg
     2821 gactgcacag gtagatctcg gaatccttgc agccgaaacg gcccttgaaa ttgcggtttg
     2881 acgtgctcag gacgcgttcg ccgggggcga gaatgccctc atggttgccc atgcaggggc
     2941 cgcagcccga gtttacaatg gtcgcgccgg cttcgccgag aatctggatg tagcctttgg
     3001 ccagggcttc gaggaagacc tgacgggagg cgggaatgac caccatccgg caggagggat
     3061 ggactctctt gcccttcatg acgccggcga cgatttcaaa gtcctcgatg cgcgcgttgg
     3121 tgcaggagcc gaaaaagacc tggtcaatcc tggttccctt gaccgcagag accggcttga
     3181 cattatccac actgtgcggg caggcaacca gaggttcgag atcgctgaca tcaaattcgt
     3241 ggacgctttc cagttcggcg tccggatcgg aggtgacgac ggtgaagggc gcgcgcgccc
     3301 gccccttgag gaaggcgagg gtctggtcgt ccggctccat atagccgttt ttggcgccaa
     3361 attcgaccgc catattggtc agcaccatgc ggctggagac gctcatctct gcaatggcgg
     3421 gtccagtgaa ccagaccgac ttgtagagcc cgccgtcggc gctgagatcg cccatgattt
     3481 taagcatcac atctttcgat gaaatccact tcggcagcct ccccttgacg ataatcttca
     3541 tggtctcggg gacgcgcagc cagatcttgc cggtggcaaa aatcaccgcc atctcactgc
     3601 ggccgatgcc ggtggcgagg gcgccaaaag ccccgtaggt ggtggaatgg gaatcgctgc
     3661 ccaccatcag atagccgggc agggcaaagc cctcctccgg cagaacctgg tggcagatgc
     3721 cccggtcgat gtcgaaaaag ttcggaatgc cctgctcggc cacaaaggcg cggatgtcct
     3781 tatggttctg ggcgaatttc tcgttggctg ccggcacaca atggtcgaga acaatgacgt
     3841 gccggttggg atcatcgatt ttagccacgc cgatctcctt gaaggttttg gaaatcgccg
     3901 cggtgttgtc gtgactcatg gcaatatcgg gcttgacttc aacgatctcg ccggggacga
     3961 cgttcttgcc cgctttttgt cccagtacct tttctgcgaa ggtctttccc ataccgcctc
     4021 tttgccggtt acgtggtttt ggctgactaa ttacaataaa agtaaataaa aaagggatgg
     4081 aatacaagag ttattcattg agccagacaa aatgaagaag cggctttcga cacgccacgc
     4141 ccccgccggc gccggcgggg gcgtactgga ttcatcctta tttcatcagc accatcttgc
     4201 cggtgcggat gccggctgga gttgagagcc ggtaaaagta gatcccgctg gtcatggtct
     4261 ccccctgctc gctgcggccg tcccaatgca gccggtgctc gccttccggc agggcgcctg
     4321 tatgcagctg gcgcacccgg cgtccgagcc tgtcatagat ggccagggtg acctgcatgg
     4381 gcgaggggag tgaaaaatgg atgctcgtct ccggattgaa gggattgggg tagttgccga
     4441 gaagcgcgac cttgtcggga aggaccctga catcgacgcc ggtgctccgt gccggcggca
     4501 gggtgatgtt cacctcggtc aagccgttgg ctgcgatcag gggggtggtg gcctcgcggc
     4561 tggaggcatt gccgttgaag gtcgtggcat agccgagctt gccggcctgg agataatagg
     4621 gtccgttggc gatgcccata atctcaaagc ggccattttc gtcggcggta gcccaggtga
     4681 ccggctggcc gctgccgtcc aggaggtaga cggcggcgcc ggcgatgccc tggccattct
     4741 cgtcattgac cgtgccggaa atgatggcgt tcaaggcatt gcggccggcg gcgtctccct
     4801 ctttcataaa ataccaggga ttggcccgca gggtgaaatc gatgccggtg atcacctggc
     4861 ccgcttcaac cttcaccagc accgcttctg ccggatcgaa ggtctccttg tagtattgca
     4921 gcatgtggcc aggagcgaaa ctggagagat aatactcacc atccggcaga cccttgagag
     4981 tatagctgcc atcggggtcg gtgacggcgg tatagaactg ctcggaatcg ggccatagca
     5041 tgatggtcac cgcgggcttg gccataacga cagcgatctc gggaatatat tcatcgagga
     5101 cggcctcaag ccggacattt cctgggcggg catcgccctc tgtggcgacg aagccatgct
     5161 ccgcacgcac ctggcccgat atcgtggcgg tgccgctctg gcgcagggtc agggcaaagt
     5221 tggcttcgct ctttctgccg ccgaccacat ccaccggggc ggccatctct tccacgaggg
     5281 catctggata ccaggcgaag gcgccgttgg tgtagaccga gagcttgtag ctcccctcgg
     5341 gcagggattc gaagacgaaa gctccctggt catcggtgat ctggtagtag ggccaccagg
     5401 cgaaacgacg gatggcataa tcagccgaat cctggcggta gtcgacttgc acataggcgc
     5461 gcgggagcgg ttgaccggtg gcagcgtccg tcacagtgcc aacgatggcg ccgtagatgg
     5521 ggtggacatg cagggcaaag tcgatgtcag tccggttttc gccctctgtc aagacgatcg
     5581 gcgtcgccga cgcgagggag ccggcatcct tgtaccaacc ctggccgaag ctatcaccct
     5641 cccagcaggt cgcgcaggcg atatagcttc cgggggagag gcggtcgacc ttgaagaggc
     5701 cgctgctgtc ggtcgaagcc caggcgtaaa aactgcgtcc ggcggccgaa tcgtattcat
     5761 ttcccgccgt ggtgatctga atataggcgt tggccagggg gcggccttca gtgttgacca
     5821 cgcgaccgcc gagcgcagcg gtgcctggcg tcaggggcag ggtgaaatcg gcctgccagc
     5881 ttggcgtctc atcggagaga gtaatgattt tggcgctctc catggtagtg gcgtccggcc
     5941 accagagctg aatataatcc cagccgttct gagcccagca gtaagcgata tagctgccat
     6001 ggggaacctg gtcgatctga tagtatccgt cggcatcggt gtcggcgtag agccagatcc
     6061 agttccagcg gatgttctgg tccggtgcgg ccatgacctg gattccggca gcggcgagag
     6121 gattgccgag ggcgtcgacc accctgccgg cgatgcggcc ggctttggat ggcacctgga
     6181 aagtaaagtc gatgccgctg gtctcgctgt tttcactgac tgtcaccgtg ctggcatccg
     6241 cctgggagtc agcctgagga taccaaaggg tttggccaaa ccaggtgttc cagtactcgg
     6301 cccggaggta atagtcgtcc ggaggcagat tgaccatagt atagcggccg ttttcatcac
     6361 tggaggcata gccgatatcg ataaaatctc tcttttcggg atccagcata aagagggttg
     6421 aggcaataat attgacaaaa ggcagcggca ggccatcgga gccggtcacc tggccgctga
     6481 tggtgccgcc atggacgagc gtgcccggct ttaattcaaa ggtgaggccg ccggtcacct
     6541 gtccgggata gacagtgaca agggtggctg cgctccagta tgcgacccca ttgtaatact
     6601 ggggcaggta gcccggggcc tcggcgacga cataatagcc gccgggggcg aggctgtcaa
     6661 tgcggaaaga gaagaggctg tcgaccacgg cgcggccttt gccccactcc atgatcgagg
     6721 gatcgttgct ccaggcggtg accgaggcga tgccgatgag gctggagtcg aggccgctga
     6781 tccggccgct gatggatccc ttttccatcg ttgagacggg gctgccgccc gcgtcctggg
     6841 ccgccggccg ggacgctgcg cccagaagga gcagcgctgc ggtggccacg agggcaaaag
     6901 atttttgagc gtatttcatg gtagtactcc ctggattggt gcggaactca ggctggcctt
     6961 gtctgcgccg cacggtgata aaaagcgggg agcatgtccg gggcggatca tgccctaatt
     7021 ccattccggt tgatagatac gtccgacaaa gagggcggtc cggctgtggt gatcatgaat
     7081 gaccaccagg aaggggcggt cgatgcgcat gaagaatccg cctggtccgc cgatcgaggt
     7141 tgtaccgacg ccgaccaccg tcacggcggc ggcttcggtg ccctcctcgt ccacctggac
     7201 gaaggaggag tgtttcacat aactgataaa caacggcatc atggtgctga ttccggaaaa
     7261 atcggcgcgg tggtcgtcaa aggcgaccgc catgcccatg gctttgaggg cgttattcat
     7321 agtcacatca caggagagtt tgaatttggg aagctggagg gtgccccgct gccggctgaa
     7381 gctggagtgc cactcgtccc aggccgcatt gtcaagacgg ctggtcaggt cctcgagagt
     7441 ggtcctgggc agaaggaggg ccatggcgaa ctgtccgtct ccatagggca gatccacagc
     7501 ctggaagagc tcgttctcga agtagggcag gttgtcggtc agctgcatca tctggcagga
     7561 ggcctggctg ctgtcggcca gaaaaaagcg gtccggcctg gtctctgagg gatcgaattc
     7621 ggtggtccaa tctcccttga aatagatcgc attgagcagg agcatcatca aatcgggatc
     7681 gatggtgtcg ataaccctgg ggatgcggcc gttggtcttg ttgctggccc agtcgttgat
     7741 gatcgagggc gcctgcgggg agttaaagtc caggggggca atggtcgcat tgtaggcgag
     7801 ccggttgcgc tcggtgaaat cgggcttgac ctgcaatccc tgacggtacc aaatggcatt
     7861 ggcgatcctg aactggacgg acggatcgag gcccatcagc aggctggaga gcccggagag
     7921 ggcggcattc aattcatcgc gggagaggtc gggaaatccg agggtggtgc gcatggcttg
     7981 ctcggtttca ccgccggcgc cgttataggc catcgcgagc gccagggtga cgctgagggg
     8041 ggagatgaag ctgttttttt gcccctcttc cagggccatt ctctgcacca gccgccagcc
     8101 gaattcgttg ctgtcgcggg cgagggcgct ctcgtgcgcg gtgagtggac gggcgggcgg
     8161 attatcccgg ggatcggtcg gggaggcatc tcgggagcag cccgacagaa taaacaggag
     8221 ggcgaggggt aaaaccaacc atgggcgttt catggcgatg actccttgca gatggaaagg
     8281 cctatgctct gaattaacaa gattgatacg atgtgcggat gcgggatgtt cccggagcag
     8341 agctctttcc ttgcgaaggg gaggggatgc ttgtttacaa ctgccgatta aatgcgaggt
     8401 tactgggggc ggttggcgaa gaggggcttt ttttatcggc cgacaccttt ttatggcgat
     8461 tatctccttg caaaagaggg gataatttta taatatgata aaatggattt tgcgctttgt
     8521 cgaagagggt tgccatgtct gaccgtttgc cggtgttcac tgctgtattg ggattggctg
     8581 tcctgctcac ctgcctgcag gcgagcgatt ccccgctcta tcatctggcc ccgctgccgg
     8641 tgatcgacca ggatatccgc cagaccacgg cgcgcggact gaccaatgcg gaaaagatgg
     8701 tctattactc cgggcgcttt atgggtgcgc cctatctgct ggtctgtgaa ggggagggtg
     8761 aaaacggccg ctatgagacc cagcctctcc tcaatttgca aaagattaac tgcatgacct
     8821 attgtgagat cgtcatggcc ttgacgctct cacgcaatta tgaagagatg ttcaacgttc
     8881 tccagcatat ccgctatcgc gacggttata tcagtatggc cacacgcaac cactatacta
     8941 tggtggactg gttgccggct aacagctggt gtcttgagga ggttacccgg caggttggcg
     9001 gggcggacgt cgtggaatcg acgcgcacca tccgccacgg tctttttttt cagggcaagg
     9061 gtattgatga cttgcccgcc gtgctccctg accggacagt gtgcgcggcc tatatcccgc
     9121 tgcaaaagct cgcgctgcat gagcaggcgc tcaacagcgg ggatgtcgtc gccctgatcc
     9181 aggacaagcc ggatattttt tcggcgcata tgctcatggt catcaagcga gagggacgca
     9241 cctggtttcg tcatgccagc atgagtgcgg ggaaggttgt ggatgagccc tttgcagagt
     9301 atatcgcaaa gatcagcaaa aggccgctct atcagggcat gagtttcatg cgcatcaggg
     9361 agcaggtgga ttggtctctg ccgggagcag cgcgcggaaa gaatccgctg cacgagtaat
     9421 cgcatcgatt gggaggcagt atgggtggag tgaagatggg ggtgggcgtt ctcgtcacgg
     9481 tttgtgctgt ggtgctgggt gtggtcggct gcggtgatca tgaactctcg gcgacgacgc
     9541 aatcggctgc ggccttgtgg gagctctcgg agcgcgccgt gttcaaggag gggcggcccg
     9601 cgaatacttt tgtcgccacc ctgctggaat ccctcgccgc cacctccggg cagggcaagc
     9661 cggtttctga gggcgaattc agggagctgt tgggcaggct ctcccccgag gtctatcccg
     9721 agaacatcat caaatacgcc acgccccaga gcgtgaccat ccagaaccac gagcatacgg
     9781 actaccgcaa ggtcttcctc aagccgcgcc ggatagcgga gggggctcga ttcatcgccc
     9841 gacatcgcgc cgagctggac agtgcgcagg cacgctaccg ggtggaggtg cgggatatcg
     9901 tctccatcct gatgtgggaa tcgggcctgg gcgagtttgt cggcagccat tttattgtca
     9961 acgtttttct cgggcagatt ctttacctcg atcaggcccg cgatctggct gtacgcgagc
    10021 tcatcatcaa gggggaggcg gacacgacgg cgttgaagct cggggaggcg gagaaaaagc
    10081 ggctcgaccg tctgcggcgg agcgccgcgc tcaacctggc ggccctcctc cgcatgagca
    10141 aggagaaggg ggtggaggcg gccgaactgc gcggctcctg gggcggagcg attggctacg
    10201 ttcagttcat gcccagctcg atgcagtttg cgagggatgg cgacagcgat ggccgcatcg
    10261 atctctgcac ctggccggat gccatccatt ccgtggccaa ttatctgcat gcgaacggtt
    10321 atggagcctc cgggaggggg cggcgacggg ccattcatgc ctacaatccg atcgactcct
    10381 acgtcaacgg ggtgatcgaa tacgccgatg cggtctggaa aaaatatcag gaagatactc
    10441 tacagcgata gggagtccca ggcccatggc caggaggcgt cattataccc ggttcaaatt
    10501 tgagggcacc ttcaccaggg gctatgatct ctttaaccat gtcgccaccc tggggcttga
    10561 tcttttctgg cggcgcaaag ccgcggagca ggtgagccgc gacctcaacg gcacggcctg
    10621 tccgggggcg gtgctggatc ttgcttgcgg caccggggat atggctgccg ccgtcgcccg
    10681 tgcgcacccg ggggtgcagg tggtcggatc ggacccctca gcggcgatgc tgcattacgg
    10741 cagggagaaa ttcgagacct ggaatggccg cattgccgtg gtgcaagcgg tctcccttct
    10801 gccctttccg gacggcgttt tttcggcgat cacctgtgcc ttcggtgtgc gcaattttgt
    10861 cgcccttgcc gatgatctgc aggagtccct gcgtctgctg cggccgggcg ggcggctgaa
    10921 tgtgctcgag tttttcatcc ccggcaattt gctcatcaag gcggtgctga agagttataa
    10981 tgccctgatt tttccatttc tgggttttgt gttgaccgga cgtgttggtc cttatcgcta
    11041 tctattcaac tcgatattca cattccggac cgtgcccgac ttttgcactc tgctcgaagg
    11101 cgccggtttc agggatatcc gcatcaggag tttttttttc gggatggtcc atctggtgag
    11161 cggcggcaag ccgcagtgac cggccagtga cttaagaacg aggaatccaa acatggcgac
    11221 gctgaaagcc tattttctgg ccacccggcc ctggtcttac acggtgagtg tgctgccgcc
    11281 cgttttcggg ctgctgatcg ccatcatcga tgtggccggg ttgcaggtag actgggtcaa
    11341 gttcgtcctt accctgctcg gctgcaatct cgcccactcc gggtccaaca ccctctcgga
    11401 ctattttgat ttcaagacca gggtagaccg cgaggggact tacggctcga gcggcatgct
    11461 ggtggccaag gttatgaccc cgcctgaaat gctgcgctgg gccctgatcc tttatgccgc
    11521 cgccggggcg atcggactct atttgatcct cagctcggga aaccctggcc tgctcatcgc
    11581 cctggtcgca gccggtttca tcctgggttt cttttacacc atggccccgt tcaatttcaa
    11641 gtatcatgcc ctcggggatg tcggggtgtt tctcgccttt gggctgctga ttccgctggg
    11701 ctcctacgcg gtccaggccg gactcttttc ctggaagcct ctgctctggt gcattccggc
    11761 cgcgctgctg gtggatgcca ttctgcacag caacaatctg cgcgatatcc ccttcgacag
    11821 cgccgtccac atcaggacgg tgcccatact catcggcgag cgcagggcgc agacgatgta
    11881 ttacgtgctg gtcctgggcg cctacgcctt gattcccctg ctggttatcg gcaccgggct
    11941 gacctggttg agcctgctgg ttttcctctc cctgccggtg gcgctgcaga atattcgcac
    12001 cgtacgccgc aagcaggaaa tggcgattga gcagtttgcg ggcatcgacg ccgccaccgc
    12061 caagctccat ctgcttttcg gcctgctctt catcgccgcc cttgccggcc ggatcttctt
    12121 tctgccatga gagacgccat gcataaaagg atcctctaca ctgccatcgc tctggccttt
    12181 gttctctggt acatcatgtt cgtgctcagg cctttcaact tttgggcgat gatggccttc
    12241 agcacctctc tgctctcgct ggttgtcctt tggattggca agccctttgc cgaccgtggg
    12301 gaatggagct ggagcaacct ctggctagga gtgacttcag ccctgctgct ttacgccatt
    12361 ttctgggccg gaaaccaggg gctgattctc atggaaaggg tcgtgcccgg cctctttgcc
    12421 gaccggccgc agaatctggc ggcgatttac ggcaaccgtg gtgagttggc accccactgg
    12481 gttgctctgc tgcttttttt cccggtcggc ttcggtgagg agttcttctg gcggggtctg
    12541 gtacagaagc acttcgcagg caccctgggc cgctggcgcg gttttctgat cgccaccttt
    12601 ctctatacgg ccgtccattt tgccaccggc aactatgtat tgcttttggc cgcattgacc
    12661 tgcggcctct tctggggggc tctttatgca tggcggggcc atctcttccc ggtcctgttg
    12721 tcacacatgc tttgggatcc tctgatattt gtcctctttc cgattcaata ggagagaatg
    12781 ctgtgagaga cggggaaaat ggcgggaaat gccggggcgg ggagcgtcga gggcgctgca
    12841 gcgagcccct gctctctctt ctgatcctgc tcctgctgct gccctggaac aggggggaag
    12901 cgcagcccgc atccagtcag ctccctgagc ccattctttt gcaccgtttg caggaagata
    12961 tcgctgacga gaggctggat gaattcaccc ttatccaggc ggctttcatc ctcagtggcg
    13021 ccgcgaccga ggacagcttg gccagctgtc tctcctggta tgaggaggtg ctctcgcgcc
    13081 tgcgcgcact ttatctggat cccttcaagc ggcttgaatc agcctcgacg atcttcagct
    13141 atctccattc tttttatctc cgggaataca aggaagaggc gacaaccctg ctcgatgtcg
    13201 tccaccggca aaagttcaac tgtgtggccg ggaccattct ttacaatctc gtctgcgctg
    13261 atctgggtta cgccaccgag gcctttgaga ctcccaccca tgtctatacg atctttcccg
    13321 actttacccg tacggtcacg gtcgagaaca cttcgccgat gggattcaat atcatgcgca
    13381 atctccaggc ctactccgcc tacctgttga actactatcc ggagcatcgt gccgcgcaga
    13441 tcgggctgga tcgcatctac gcctatgaaa attcccgggg ccggcggatc aacaatacag
    13501 agctgctcgg cctgctggcc tacaaccgcg cctatttcgc tcgggagcgg aacgatttcg
    13561 ccgccgccta tgagtttgtg ctgctggcgc aggagttcaa ccgcgattcc cgatccaata
    13621 tacccttcga acagagcctc tattttcagt ggggcaagac cctttacgaa gccggttctt
    13681 tcgatgacgc ctttgcggtt ttcgccgacg gtgcctaccg ttacccggag atgaaggaac
    13741 tggcccagaa cagccgtgcg gcctattttc aggccctgcg ccggcatggg caacagctga
    13801 actggccgga gagtcgccgt ctggtcatgg agatgaccga gctggcgctg ctgggaccgg
    13861 cggagatgga gcagcagcaa gagatcctct cgggatgggc cgaatatttt taccggcgcg
    13921 ccgaacggag gccgctgctg gaggtcatcg aactgatgca gtcggcccat cccgaagagc
    13981 cgcgcctgca ggagatgaga cgggtggccg aacggctgcc agaatagcta accgcccttc
    14041 cgtgaagggc aggagcagat tatgccgata tacgatctgg aggtgttttt gcgcctctcc
    14101 gccgatttga actacctgct ggtggtcaaa ccgaccaact ggtcgagtgt tctcgacaag
    14161 attctcaacg aacagcacct gccggtcgcc aagcaggaga tcctgctcga gaccatggag
    14221 tatctcgatc aggtttacgg ccggaagcgg cggcgcatgg gcgctcaggc gatcatccat
    14281 cccctgcgtg caacggccct gctggtgcgc agcatgcctg tgccgctgca tctggatatg
    14341 atggcctctc tcctgcacga ccggttcgag gattttccct ccgtcagccg gccggctgat
    14401 tttggcgaat ctatcgatcc acccttgcgc gcgatcatga acaagcttcc agaggacgag
    14461 caatggttcc tgaccgagcg gctctactgg ctgaccaaaa gatcaggaga gaattattac
    14521 cgctatatcg gccgtatgct cagcgaagcc tgccggacgc ccgaggctgt gcgcgtcaag
    14581 ctggccgacc ggcttgataa cactctcgac atgcgcatgg attttcgcca cgccaatgac
    14641 aatatcgatt ttttcgaaga gctttttcag atgatgtttc tcccctccta tcatcccaat
    14701 aaaagcaatt ttgcccaccc ctctcactcg gccatggatg gctccgagcg cctctatcaa
    14761 ctttacaaaa acattgtcct catctcgctt gtccggcaga aaaaggccgc tgaagatgat
    14821 gccatctcca tcaccctttt caaaatcctt attcaagcca gcatcaagga ggcgaaacgg
    14881 attgcgcagc atatcttctc ctaccaccag accaacatca cccgcatgcg cgagctggtg
    14941 atcgagacca tgcagtatat catggaaggt ggaatcgatg cggtgacggc acccaccctg
    15001 ggcaagatgc tggacgggct cttcctgaaa tggttcaacg agccggaccg ccgcgtccgc
    15061 accagaaacc tggccgaact ctacaaggac aaggatctga tggtggaagc ctccatcgcc
    15121 ttcatcgtta ttttttatcg ttttctcgat gatcccgcct actttgtgca cggcatcagc
    15181 gaagagggca tcaaggtgga ataattttcg cccaggcagg cggccgcccg gctcacccat
    15241 attttgtttt gaattgttcc gcaaaatgga taccttaatg gttatcgaat cagtttcctt
    15301 ttaactcccg aagaggacag agatgttaaa gacaatgcaa gagaaggcgg gagggcttgt
    15361 tctgcttctg gctggaatcg gcatgatgat cagcatggct tccaatgcag cggccggggt
    15421 tccgcagagt gcggttgaac ggacgattgc cctcctgcag agccggcatg aagcgggcgt
    15481 gcaccagcgc atcgagctgg gcgtgcgtca ggccgcctct ctgtggcggg aagaggacgg
    15541 cacggagagc gagttcagcg atttttgcgc cgcccatttc gtcgccgatc cgcagcagcg
    15601 ggcggccctg ctggatcgct ttgagagcaa ccttgagacc ctgtacggcc atgcccacga
    15661 ggtgagccgg gcatggtcca tccccctgca gcttgagctc ggaccggtgc tgcccgtcga
    15721 ttacctcttc gccgaataca gcccctttgc ccatatagcg gatgatcttt ttcacaacaa
    15781 gatcgccttc tccgccctgc tcaattttcc attttacacc ctggaggagc ggctcaggct
    15841 gggcccctcc tggagccgtg aggagtgggc agagatgcgg ctggccggac aattcagcgc
    15901 ccgggttcca gcggaggttc tgcaggcctc gagcgtcgcc tatgtccagg cggatgacta
    15961 tatctccaac tacaatatct atatgcatgc tctggttgat gagcagggcc ggaggctttt
    16021 tccggagggg ctcaagctga tcagccactg ggggctccgg gatgaactca aggggcagta
    16081 tgcggagaag gaagggctgg cccgccagcg catgatcagc aaggtgatgg agcgcattat
    16141 ccgccaggag attccgcagg cggtgatcaa cgcccccgtc ccgctttggg ctccctacac
    16201 caacctggcc ggggaggagg gccggatggc gccagcggcc ccggaagagg atgtgcgcta
    16261 ctcccatctg ctggcgatct ttgcggccga atgcggagtc gatccctatt atccaggcca
    16321 gccgactaaa atcgcgcgcc ggttcgaccg cgagcgggag atccccgagg cggaattcga
    16381 gaccctgctg atccgtctgc tcaccgatcc catcgccccc cgggtggccc ggctcatctc
    16441 acgccggttg cagcgccccc ttgagccctt tgacatctgg tacaacggtt tcaagccgaa
    16501 gagcgccttg ggcgagagcg agttggaccg cattgtcagt gcaaaatacc cgaccacagc
    16561 ggcttttcaa cagggtttgc cggagatcct cacgcgcctc ggattcaatc ctgaaaccgc
    16621 cgcctatctg gaatccaaga taacagtcga cccctcgcgc ggcgccggcc atgccatggg
    16681 agccatgcgt agggaggaca atgcccatct gcgcacccgt atcccggaag gggggatgcg
    16741 ctacaagggc tacaacatcg ccgtccacga gcttggccac tgcgtcgagc aggttttttc
    16801 cctgaacagg attgatcata ccctgctggc cggggtgccc aatacggcct tcacggaggc
    16861 tttcgctttt gtcttccaga gccgcgatct cgagctgctc ggcctggcgg ccccggatgc
    16921 gcaggcggcg cacctcaacg ctctggacac ctactggggc gcctgcgaga tcgcggctgt
    16981 cgggctggtg gatatacgcg tctggaactg gatgtacgaa catccggatg cgaccccggc
    17041 cgagctcaag gcggcggtcg tcgagatcgc ccgccaggtc tggaacagct atttcgcccc
    17101 cctcttcggg gtgcgcgacg tcatcctgct cgccatttat tcgcacatga tcgacagcgg
    17161 cctctacctg cccgactatg ccctcggtca catcatcagt ttccagattg agcgctatct
    17221 tcaggacaag aatctcggaa cggagatgga gcggatgtgc cggctgggga tgctcacccc
    17281 ggatggctgg atgagcgcgg cggtcggcag tccggtctct gtcgaacccc tcctggaggc
    17341 ggtccgcgag gcggtcaaag tgataaaaaa gtgatgcgat tcctcagttt gaccatggat
    17401 gagggcgatg cctcgcggcg tcgatcgcag cagaactttt tgcgttgtga gcagggggag
    17461 atcacccacc ttcacagcat gagagcccta tgagaccagt caagacactc gtaattttgt
    17521 ccctagttgt gctcttctcc ctcttgccag ggaaggctgg cgcggacgcc tattcttatg
    17581 gacctgcgcg cctgatgcag gacagtctgg cccgccgcaa cgccatgccg ctcgatggtg
    17641 tctggcattt gcagatcagc ggcggggatc ggcgttctct ggagatgccg gccgcgctga
    17701 ggctgcgcga gcagtgcacc ctcgagaaaa agttcgcgct ggacagcacc atgatctcct
    17761 cttcttatga gctggcgttc gacgggcttg acggcctcag caccatctat ctgaacaaga
    17821 agatcatcgc cacccattca gccggcagcg ccccgtttgc catccaggtt ggcaagcagg
    17881 atctcttcct caacgaggag aacgagttga tcatccagct cgatggccgg cttgactacc
    17941 gacgctcgct gccactgctg gtgcgcaacc gcggcattcc gctctcgggc aacgggcttt
    18001 tccgtcccct ggttctgcgc agtggcaaaa ccccgtttat cacctccctc tccctcaacc
    18061 ccgcggagag cagcggcatg ggtttgcaga ctctggacct tcgggccgtc gtcgccctgg
    18121 ggggtatgga cagcctggcg atggcctccc tcgcttccat gcgcggtcaa gtggagatcc
    18181 tggatggtca cagcctgcaa tctctctttg tttcgcccca gctgcccctt aacgccactg
    18241 ccgtcgatac cacttcgctc ggtgtgacgg cggttatacc tgccttccgg cactgggcgc
    18301 ccggagcgcc ccagcgttat cgtatcgtca tgcagctctt cctcgggtcc gaagtcatcg
    18361 accgcgcctc ggtctggttt gcccgttcac agcccggtca gtggctcgcc gcggccgggg
    18421 agaagggcgg cggtttccgg taccgcgccg tcgattgggt ggaggatgag cggcagatcc
    18481 tgctgcctca gcaggagcag aaaagcgtca tccttgagga tctgcgggga atcgtcgatc
    18541 tgggggccaa tacagtgcgt ctgccgggcg gcctgcccgg ggagtttttt ctccagagct
    18601 gcgacagcct tggccttgct gtactggtgg agatcccagt gacccacatt ccctccgcgc
    18661 atcttaataa tgctgcgatc cggcagaagg cgcggagcgc gctcaccgat atgatccgga
    18721 cctgccggag ccatccctgt gtggctgcct gggggttggg cagcggctac gatccgtcgg
    18781 atctccgcgc tcaggccttt gtccgcgatc tggcggcgat cgcccgcgag ctcgacgacc
    18841 ggccggtcta cgcgagcatt cgcggcaaga agctggctgc ccatgctttg ccggtggatc
    18901 tgcagattgt cgaggtgccg ttggagaaga ccagcacctt cgcccagggg gcgtggcgaa
    18961 ccaacgggcc ctatcttctg cagctctcct caccccttga tctgcgggat agcagcgacc
    19021 gcagcgccca gcagaaccag gcttattatc tcaaaacggc cattctggat gcgcagcgcc
    19081 gcagtcaagg tgcaggcctc ctcatttccc cctggaaaga ctggcgtggt gaggcgcccc
    19141 acacctattg ggggcctcgg caggagaccc gtctgtttgt cgccggcctg ctggacgaga
    19201 aaggccagca gcgtctggcc tgccaggtgg tcaaggcggc ttttaaaaac agcgagatgc
    19261 ctgagctgct gcccgctgat gtgccagccg aggatccccc ggtttttcag atcatcagca
    19321 tcgtccttat cgtcctgctg ctcttttata tccgcaccga caagcgcatg agccattacc
    19381 tgaagcgggt attcgtctac ccccacggtt tttatatgga tctgatcgag aaccgccagg
    19441 tcaatccctt cctgaccggc gtgatgggat tggcttcttt cctgaccgtg agcacgctgc
    19501 tggccagcct gatctttttt ttgcgcgaga acagcctctt tgatgagctg ctgacctggt
    19561 tttttccgaa cagcacagcc aagaaccagg ccatcgccct gatctggaat cccgaacgga
    19621 tgatcctgct gctgaccgtc gtcatggtgg gcctggcgtt actgcagagc attctgtaca
    19681 agctgattgt gctttggcag cggcgttatc tgcgtttcag tcaaatcctc accttcagct
    19741 tctgggtgcc cgccaatttt atctttgcct tgccgctggc ggtggtgctg ttccgggcct
    19801 tgtcgcgatc caatctggtc actctcagcc tggtctacct ggggatcatg ctcttctggt
    19861 tcatggtgcg gagtttgcgc gggaccaagg tgatcctgca gaccacgacc ttcagggctt
    19921 ttctggtcgt agccgctggg ctctttttca ttcttttggc ggccggcctc tacatggaac
    19981 agacccgggc gatgacggcc tttgcctcct attactggtc gctgctgggc cagtgatccg
    20041 gcaaaatgat attgacttta aactaatgaa gtgctatttt gaaaatctgt ccctttaatc
    20101 cgaaccaatc ataacggggt tctgtgtatc tttccgaatt gaccattgtc ggcttcaagt
    20161 cttttgccaa aaagacgcac gttaccatgc acgacggcat cacgggcatt gtcgggccta
    20221 atggctgcgg caagagcaat attgtcgacg ccatccgctg ggtcatgggc gagcagaaga
    20281 gcggggcctt gcgcagcgag gtcatgggca acgtgatatt caacggcacg gccaccgcca
    20341 agcccgtcgg catggccgag gtatcgctca agattgaaaa ttccggcaac acccttccaa
    20401 tcgactatgc cgaggtgatc atcacgcgca ggctttttcg ctccggggaa agccagtatc
    20461 tgatcaacgg caacagctgc cggctcaagg atatcctgga tcttttcatg gacaccggtg
    20521 ccggtccgca tacctattcg gtgatcgagc tgccgcaggt ggagcgtatc ctcaacggca
    20581 aggaggatga gcggcgtcat atttttgaag aggcggcggg catcaccaag tacaagctga
    20641 ggcgcaaggc cacctttcgc aagctggagt cgaccgaaaa ggatttgatc cgcattgagg
    20701 acatcatgtc cgaggtcgaa aagaatgtgc gctccctgcg tcgtcaggtc gccaaggcgg
    20761 agcgttatca ggagatcacc tccgaactgc gcgaccttga gatcaagttg gcgaacaacg
    20821 actatgcgat gatcctggat gagctggagc cggcggagac caggctggag ctgatcaggg
    20881 acgagcgcga gggggcttcg agcaccctcg ccacccaaga tgcagactat gagaccacgc
    20941 gcgcccgtct gctggagatc gagaaaaagc ttctcgaaaa ccaacgtacg gtctccgatc
    21001 tggaccggga ggtgcagaaa ctggaagagc ggatcctggt caacaaggag cgtatccgtt
    21061 ccctggagga gagcggcctt cgctcaagcc aggagcggga gcgttttgat gcccatcttg
    21121 tcgagctgca gcagcagtac aatgccgctc aggagctcta cctcgaagcg gagaagaacc
    21181 tgacggccag gcgtgaagag tatgaaaagg ccaacggcga gctgcaggag gtgcgggtcc
    21241 agttcgaatc caagcggagc ctggcgcggg aagcggagaa tcgtgttttg cgtatcaccg
    21301 aggagttgag ccgcaagcag accgagggag agcgtctcaa ggcgacggag gagagtctga
    21361 gccttcgtct ccgccagatc gaagccgatg ccgagaagga tgcagcccgg gcaacggagt
    21421 tgactgaaat tctgaacaag acccgtgcgg aggaggccgc cctcgccgat accctgggaa
    21481 aaaagaagca gcgcctcacc gagatcagcc ggagggaaga ggaggccaag cgtctctttg
    21541 aaacgctgca aaagagtgaa tcccaggacc gcagccgcat cgaggttctg ggtcatcagg
    21601 ctgaactggt gcggcggctg atcgacaatt acgaggatta tcccgcgggg gtgcgttttc
    21661 tggccaccct caagaaggag ggtttttcca cccacggccc cctcgccaat atcattcatg
    21721 tcgaaaacca gcatcgcaca gccatcgccg cggccctggg cgaggcggcc acctacctgg
    21781 tggtggaaga gagcaatacc gctttcaacg gcatcggact gctgcgcgag gagaggcgcg
    21841 gcatcgtcac cttcgcgccg cggcaggaga tgggggccat tctgcgcgaa cggccgctct
    21901 tgagcgatct gggggtgatc ggctgggcgg atgagctggt ctcctgcgaa gaccgttaca
    21961 gcgccctggt acgccaggtg ctcggtgctt tcattgtgac ccaggatctg cagacggcgc
    22021 atcgcgtctt tgccgagctg cgcggccagt cctacagcgt ggtcaccctc accggtgagg
    22081 tgctttcgca caacggctat atccgtggcg gggtgttgag caagaaccag tccgatttcg
    22141 tcggcaggca ggagcagctc gccgcactta cgggggagat agaaaagctc cagcagggtc
    22201 tggaggcgcg gcggattcag ctggggcagc gcgagaccga gattcaggaa aacaagatcg
    22261 aggcggaaaa gctgcgcaag gagattcagg attcggaaca agccctgggt tccctgcgta
    22321 ttcagttagg gcggcagacc tttgaggagc aggccctcgc tgaggcacgt gagaagcgtg
    22381 agggtgagag acagcgcatc atcggtgaag tgagccagct cggccacaac ctcgagctgc
    22441 agaatctggg cgccggcgac ctgcagacgc agcggcacga gagcgccgag cgggcggcgg
    22501 ccctgagcga ggagatcaag gccatcgaag aggccgtcga ccgcgccacc ggccgggctc
    22561 gcgagctgaa tgtggccatg gccaggatgc agagcgaatt cgaggcgatg cggcgggaga
    22621 gcgatggact gcaccggcag atcgttgaaa ccgaagcctt gatcaaaatg cgccgcgagg
    22681 atgcagagaa tgcatcggcg gagatcaaag agctgcacca ggtcaacgag aactacagcc
    22741 aggaggtggc caggcagcag gtgcgccgcc aggaggtgca ggcggtgctc gacgagctcg
    22801 aggagcagca gtatcaggcc aacatccgcg tggctgaggc cgaaaagctg atccgtgcct
    22861 cacgcggcaa gttcgacgtg ctttccgagt cggcccatca gattgaactg cgcgtctcgg
    22921 agctcaagct caaggcggag aacctgcagt cgcggctttg gaatgaattc gagtacaagc
    22981 tgcagcggat ggctgtggat gtggatataa accccaatga gacacatcag cggctggaga
    23041 acctgcgcga gcggatcaag gaggttgggc cggtaaatct gctcgccctg aaggagttcg
    23101 agcaggagaa ggagcggctc gattttctcc agacccagcg tgacgatctg atcaaggcgc
    23161 gcaagaatct gatggagacc atcgagatca tcaatacgac agcgcgacaa aagtttctcg
    23221 agacttttga gcaggtgcag cgcaatttct cagaggtgtt cagcaagttc ttcgccggcg
    23281 ggcgggcgag tctggtcctc caggagaccg ctgatccgct tgaatcggag atcgagattc
    23341 tggccacccc gggcggcaaa aagcccgcgg cgctgaccct gctttccggg ggcgagaaat
    23401 cgctgactgc gatttccctg ctctttgcga tctatctggt caagccgagc cccttctgca
    23461 tctttgacga agtggatgct ccgctggatg accagaatgt gcagcgcttc acccatgccc
    23521 tgcgcgagtt ttcgagcaac acccagttca tcgtcgtgac gcacaacaag ctcaccatgc
    23581 gggcggccga ccaactctat ggcgtaacca tggaggaaca gggaattagc aagctggttt
    23641 ctgttaagtt tgactcgatg aaaaatgacc agctggtgcc ggcggccagt tccgcaccgg
    23701 ccaattgagg agggtgcatg cgtatattgg ctgtgattca aaaaaacgtt tccttcatcg
    23761 cactgctgct cgcactcgcc gccctgccac tgccggcgca ggaggagaaa agtgaggaga
    23821 ggctgcgatt caatatcgac tatacccgtt cccgttattc ggacagcctc tccttttgcg
    23881 aattctacgc actgcttccc cggaaacagc tcacctatct gcccgatggc ggcagcttca
    23941 aggccgaatt caaggtgacg gccgaagtct ttctgcagga ctccctggtc tcctccaaga
    24001 tctggaagaa tgtcaaccat actgacagcc tctccaccat cgaatcgggg cagaatctct
    24061 tctttctcaa ccattttata ctgagcgagg gcgactatga gatgtccctg ctgatcgagg
    24121 atctgctcaa gccggagagc aggggcagtt atcgttttcc actcagcatt acggccttca
    24181 agggaccgga gctgcagatc agcgatttgc agatcagcag ctccatcgtc cgggacacca
    24241 cccgcacctc ctttctgaaa aacggtttcc aggtcacccc caatccctcc gggctttacg
    24301 gtctcgagct gcccattctt tatctttact ctgagatcta ccatcttgtc cccgccagca
    24361 gtgacagcgg agggcagtac agtgtcgcct acaagatttt caacgcggat ggacgaatgg
    24421 tcaaggaggt tgccgccaaa cgccgcaaca agccgggtga cagcgccgtg gaggtcgcgg
    24481 ccatcaacgt ggtcacgctg gtctccgggg cctatcgtgt ggtcgccgag gtcaccgata
    24541 tggagaacgg tgcagtcaca tcaggcatgc gcaagttctt tgtctaccgg gagggggact
    24601 atgccgaagg gggtgcggcc ggccagacaa aggaggaggt cccgaagacg ggcagtgcgg
    24661 ggatggatgc cgaccgttac gcagtgctga gcgagaagga gctcaatgag gagtttgagt
    24721 tcacccgcta catttccacc aaggaggagc gcaagacctt caagaagctt aacctcgatg
    24781 gcaagcgcgc ctttatgaag gagttttggg cccagcgcga cccgagcccc ggttcgccgg
    24841 agaatgagtt caaatatgac tatctgacgc gcattcagca ggccaatacc tcctaccgcg
    24901 gcactttcag ggagggctgg cgcactgatc gcggacgcgt cctgctggtc tatggccctc
    24961 ctgatgaagt cgagcgctac cctttcagca atgataatcg cgcctatgaa atctggcact
    25021 attactccct gcagggggga gtgcagtttt actatgtgga caagcgcgac accggggata
    25081 tggagctggt tcactccacg gcgcgaggag agatttacga tctggactgg gaacggtgga
    25141 agaatcccaa tgagtaggga ggaaggggaa gagccttccg tcaggcggct gtgatggtac
    25201 ggcgacgctg atgaggatga agaaactcgc gcaaaagtgc ctcggcttta tccagctcac
    25261 ccgtccggtc aatgtactca tcgccgggct ctccatcttt ctggcgggtt atctctgcgg
    25321 gatcggggat gccgggacca agctgctggc ggcctgtctg gtggggatgc tgatcacggc
    25381 ggcggccaac gggatcaacg attattacga catcgagatc gatcgcatca acaaaccgcg
    25441 tcgtcccttc ccgtccggtc gcctccggcg cggagagggg ctggtcctgg tcgcgatttc
    25501 ctttgctgcg gcgctcgtcc ttgccgggcg catcaatccg gcggccctgg ggctcaccct
    25561 gttctcggga tttttgcttt actggtacag cgccaggctg aagcggacgg tgctgtgggg
    25621 caatctggcg gtgagtctgg tgaccgcgct cgcctttgtt ttcggcggaa tcgctgtcgg
    25681 ccggttccgc tacgccctgg tcccggctgt tttcagtttt ctgatgcatt tcgggcggga
    25741 gatcatcaag gatatggagg atgtcggcgg cgacgccgcc ctgaatgccg gcacctttcc
    25801 agtcgtgcat ggtctagcac cagcgcggcg gctggcgacc acggtgctgt tattgctgct
    25861 gccggtcacc tggctgccct ggtttctcgg cctctacggc cgctggtatc tgctgacggt
    25921 ggtcgcaggc gtgcattcgg tgctgctgct ggtgatctgg tcgatctggc gccgtcctgg
    25981 ccgcacgaca tataaatggt cgagcaatct gctcaaggcg gatatgctcg tcgggctgct
    26041 ggcgatttat gccggccgct ggtgagggag caatcatgcg ggtactgctg cagcgtgtca
    26101 gccgggcgca ggtgagtgtg gggggagaaa ccatcggccg catcggccct ggactggcca
    26161 ttctgctcgg cctcaaggag gatgacaccc ccgaacttgc ggagcggatg gccgacaaga
    26221 cggcgcatct gcgcatcttt gctgatacgg agggcaagac caatctttcg gcgctcgata
    26281 ccggcgcgga aatgctggtg gtctcgcagt tcacgctcta tgccgattgc cggcgcggcc
    26341 gccgccccgg tttttcctat gctgcgcgtc cggaaaaggc cgagcccttg tatgagcatt
    26401 tcatggcggc tctgcaccag ctcggcttta ctgtcgccag cggccgcttc ggcgccgaga
    26461 tggtggtcga gatctgcaat gagggaccat tcaccattct actggacagc gacgaacaag
    26521 ccctatgagc ttctccctgg aaagccatct gccgcaccgc acctgttacc tggcctcgca
    26581 gtccccgcgg cgcaagcagc tgcttcagct catcggtctc aagtttcacg tcctgcccag
    26641 cggtatcgat gaggaggcct gcaccgagcg cgatccggtc gcgcatgtcc agaagctggc
    26701 cctggccaag gcggaggatg tggggcggcg gattgaatcc gggctggtcg tgggcgccga
    26761 caccatcgtc gtccttgacg gcgagattct cggcaaaccc gtcgatgagg aggacgcggt
    26821 gcgcatgctg cgccggctga gcggacgcac gcaccaggtc tataccggtt tcgccatcct
    26881 cttccagccg gaggatgaca aggtgaccgg ctacgaggtg accaacgtcc gtttccgtcc
    26941 cttgcaggag tgggagatcc gcagctacgt cgccaccggc agtccaatgg acaaggctgg
    27001 agcctatggc attcaggatc agagcgcgct gtttgcggat cgcatcgaag gctgctttta
    27061 taatgtggtg ggatttccgt taacccgatt ctacttaacc ctgttgaatt ttataaaaga
    27121 gcatgcttat gaatgaagtt tttaaaaacc tgggtgagga gcttgcccgg cagcggcagg
    27181 agaaagagct ctccatcgcg caactggcgg cgcggaccaa aataacggaa gctttctttg
    27241 aaaaaatgga gcagggggat tttgcgtttc taccccctgt ctatgttcgg gccttcatcc
    27301 ggtcggtctc gaccgagatc ggcctcgatc ccgaggtgat gatgcggcgg ttctcggatg
    27361 aggccggacg actcgccgct gcctccagcg ctcaggccgc agaggccggg gttgaacccg
    27421 ctgcggagag gcccgcctac aagccccgag ctcagtttga gcccacgccc gacgaatccg
    27481 gggacaagaa acgggcggac atggactggt ttaaaagtcc ctttaccctg ggaatcatgc
    27541 tcatcgccct tcttgccgcc ctctacttct tctttggccg caagcccaat gtggagccga
    27601 gcgcgatgga ctcggggatc gccgttgtag aaccggtgca ggagagcggt tccctgcggg
    27661 atgtcccgct cgtgcctttc accggagaca acagggtgga cagcacggcg gtcacgcagc
    27721 tctccctcac cctgcgggcg gaggagaccg cctgggtgcg cattgtctat caggacagtc
    27781 tggtggagga gggggtcttc accgagggct tgagccggtc gtggttgagc ccggagcgct
    27841 tttatctcaa gatcggcaat gcgggcggca tccgtttgat cctcgacggc caggatctgg
    27901 gattgacggg ggagaagggg caggtcgtca cgatcgccgt gaccaaggag ggcattgcgc
    27961 cgctcgatcc tgcccaggcc ccgtcgcgat tgacgcagat gaatccctga ccccggagca
    28021 gtgatggttc ttccagagcc caaacggatc tattttcttg ccatctgcgg cacggccatg
    28081 gccagcctgg cggcgatgct caaacacaag ggctaccagg tcagcggctc ggacagcggc
    28141 atctatccgc cgatgagcga ttttctcgcg gagcaggaga tcgaggtttt tagcggattt
    28201 gacccggctc atctggagtc ggcgccggat ctggtggtgg tcggcaacgc catttcccgc
    28261 ggcaacgctg agatcgagga gatccttgac cgccgcatcc cctacatctc gcttcccgat
    28321 gccctgcgcg aattctgcat ccgcgggcat cggtcggtgg tggtcaccgg cacccacggc
    28381 aagaccacta cgactgcact tatcgcctgg atatttcaga gcgccggccg ggatccgagt
    28441 ttccttgtcg ggggcatccc caataacttt ggccgcggct tccagagcgg ctccggagag
    28501 gagatcatcc tcgagggcga tgagtatgac tcggcctatt ttgacaaggc cgccaaattt
    28561 ttgcgttaca tgcccgaggt gggggtgatc aacaacattg aattcgacca cgccgacatc
    28621 tacaacagcg tggatgagat cattgtcgcc tttcgccgtt tcgtcaatct catcccgcgc
    28681 agtgggctgc tggtcagctg cgccgactat gagactgtgc ggggtgtcag cgcacgcgcc
    28741 ttctgtccgg tggagagctt tggatttgcg gctgatgcgc tctggcagcc gcaggcggtg
    28801 cgggtgaccg aaaagggatt gagttttggc atcgtccatg ctggggagct tttgggcgag
    28861 gtggaggttg cgcttgcggg tgaacacaac gtacgcaatg tgctggcagc agtcgcggtc
    28921 gcgcggaatg cgggcatcga ttttggttcc atccgctccg ccctggccgc cttcaccggc
    28981 atccgccgcc ggctggaatt aaagggcagt gagggtggtg ttgccatcta tgatgatttc
    29041 ggccatcacc cgaccgccat acaggagacc ctgcagggat tccgccagcg ccatgccggg
    29101 gcgcgcatct gggctctttt cgaaccgcgc tcggcgacga cgcggcgttc ggttttccag
    29161 aaggagatgg ccgaagcctt tgccaaggcg gatgccgccc tcatcgcccc ggtcgaccgg
    29221 ccggacaaag ccccggccgg gcagcttttt tcgacggaga tgctggcgga tgatctacgc
    29281 cggcgaggtc gcgaggccct tgcggtctcc tctgtcgatg agatggtcgg ctatctgctg
    29341 gcccatctac ggccggggga cgtagtgatc tctttcagca acgggccttt cggggggatc
    29401 catgaccggc tgctgaaagg cttgaagaaa aagtgacatc atgggagtct ttacaataaa
    29461 gtgaggttgc agagatggag agacgagaga gaagaggaag aagagatcac cgagaggatc
    29521 gtcctcgcga ggaggtcagt gagaagattt tgcagttgat caagcaggcg gaggatgacc
    29581 tggtggattc actggcgccg gt
//
LOCUS       DAOPWU010000006        29424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_1524_length_29424_cov_5.434301, whole genome shotgun sequence.
ACCESSION   DAOPWU010000006 DAOPWU010000000
VERSION     DAOPWU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 29424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29424
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1524_length_29424_cov_5.434301"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..748
                     /locus_tag="PKM23_00910"
     CDS             89..748
                     /locus_tag="PKM23_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNY90058.1"
                     /translation="MQAILFDLDGTLVHTAPDVHRAINYARGVLGLPPLSLAQALKAI
                     GPGSDRFAQTVLGDEYAQLLEEYLEVFRPYYLQICAENSRPFPGIVELLDALKEYRLA
                     VVTNKRLVQSRALLEAIDLLPYFELLVGPELVDHIKPAPDMIHYALDQLGLAPDQAIM
                     VGDTDNDLLAARAAGVKNCAVTWGYSDIAFIHTLKPDHLIDKPEELLPLLASYNHQPR
                     K"
     gene            756..2573
                     /locus_tag="PKM23_00915"
     CDS             756..2573
                     /locus_tag="PKM23_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HNY90059.1"
                     /translation="MNILGLSCFYHDSAAALIQDGEILAAAQEERFSRIKHDHDFPRQ
                     AIASCLEQAGMTVANIDYIVFYEKPLLKFERILETYLSYAPLGLRSFLMAMPLWLKKK
                     LWIPDLIRKELGFQGEILFTEHHQAHAAAAFFPSPFSEAAILTMDGVGEWATTSWGSG
                     RTNHLDLQAEIHFPHSLGLLYSAFTYYTGFKVNSGEYKVMGLAPYGEPRYVQTILDNL
                     IDLREDGSFRLNMKWFNYGAGLTMTDRRFHKLFGGPPRKPESALTQREMDLARSVQEV
                     TEMVMLRAAAHVHIQTGSKNLCLAGGVALNCVGNGRILREGPFKKIWIQPAAGDAGGA
                     LGAALFAWYQILGNPRPPRGIHDHQKGSLLGPEFSEEEIRAFLEEKKLPFRKMTTAQL
                     LDRVSDLIATGQVIGWFQGRMEFGPRALGSRSIIGDARSPEMQSRMNVKIKFRESFRP
                     FAPAVLEEEAAEWFEIDHSSPYMLLTAPVRREKRRAMTTAEKKLWGIDKLKVIRSEIP
                     AITHVDYSARLQTISAREHGLFYRLVKHFFQKTGCPVIINTSFNVRGEPIVCTPEEAW
                     RCFMRTGMDVLVLGHYLLDKQEQPPLSGDSDWRKTYELD"
     gene            2560..2997
                     /locus_tag="PKM23_00920"
     CDS             2560..2997
                     /locus_tag="PKM23_00920"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HNY90060.1"
                     /translation="MNWIKDIRDSLRQLPRDEKALQRFAALLTSLLLLAAAWLFFIRR
                     DAALTLWFVLATLLLIALALLRPRLLRPLHTGWMALAFALGALVSRMLLALFFYLILT
                     PIALLLRLLGKDLLQRKLKKEGASYWIRRPVKEVIKEDYQRLF"
     gene            complement(2994..3563)
                     /gene="rfbC"
                     /locus_tag="PKM23_00925"
     CDS             complement(2994..3563)
                     /gene="rfbC"
                     /locus_tag="PKM23_00925"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNY90061.1"
                     /translation="MKFVPCAIPDLLLIEYDVFADARGYFIESYNERAFHELGLTVPF
                     IQDNISSSVQGTLRGLHYQLHPHAQGKLVRVTQGCVFDVAVDIRRGSPFYGRWVGVEL
                     SEENKKALYIPPGFAHGFYVQSERAQFMYKCTDFYTPEVDRGIIWNDPSIGIAWPILP
                     GKLILSEKDKVRPRLADADNNLTWEGLTA"
     gene            complement(3629..4240)
                     /locus_tag="PKM23_00930"
     CDS             complement(3629..4240)
                     /locus_tag="PKM23_00930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY90062.1"
                     /translation="MRFLQGARDERLRLLGWGRSLWPLYFITRREDEAAARLLAMVPS
                     SQSWMADLGCGRGSAPARLRYEPCLGVDRSRRMVRASRYGEVGWALCADLAALPLRAH
                     HFGLLLVIGVAEYWRDPEPLLREAARLLTREGHLLLTVSPAGWHARFRRLLGSPVYYH
                     EDEILADLFTKYHIQVKARLNTPTQHLYLLSPNATAGSNNPAG"
     gene            complement(4248..5960)
                     /gene="ade"
                     /locus_tag="PKM23_00935"
     CDS             complement(4248..5960)
                     /gene="ade"
                     /locus_tag="PKM23_00935"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNY90063.1"
                     /translation="MVKELIQAARHQIKCSLVLRNARVINVYSGEIVLSDVAVYRDTI
                     VGLGSGYRGEEEVDLKGMHLAPGFIDGHVHIESSMVEVPQFGRAVVPLGTTSVIADPH
                     EIANVLGYEGIRYMMDVSKYNPLNVFFMLSSCVPSSPLESAGSELRAFDIFPFLRERW
                     VLGLGEMMNYPGVLNAEPDVLDKIKIVTDKRIDGHAPGLSGADLAAYIAAGVESDHEC
                     TTVEEAREKLALGMYIMIREGSGTKNLHDLLPLVTPENERRCFFCTDDRHPDDILREG
                     HVNYIIKSAIKAGLNPVTAVRMATLNTAEYFGLRKLGGIGPGKLADMVVFDDFDSFHI
                     HKVYKNGALVAQDGVPVYEVPVRKPAMLRSSVNIKWLEGDEFSIPVTGKRCRVIGVIP
                     DQIVTQFFEMEPSVRDGHVISDPERDLVRLYVLERHRASGNIGKGLMTGFGLKRGAIA
                     SSIGHDSHNIIAAGISDRDIMKAVTTINKMGGGIAVVDNSTVLEALELPIAGLMSNKP
                     IEFVSSRTRDLIAACRSLGIALEDPLMTLSFMALPVIPRLKLTDRGLVDVEKFAHVDL
                     FLSH"
     gene            complement(6137..7129)
                     /locus_tag="PKM23_00940"
     CDS             complement(6137..7129)
                     /locus_tag="PKM23_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNY90064.1"
                     /translation="MDLTTTYMGLKLKNPLVPSASPLSKNLDTIKNLEDAGASAIVLY
                     SLFEEQISFEAEELDHFLSYGTDSFAEALTYFPEAQEYRLGPEGYLEHIRKAKEATGI
                     PIIASLNGVSTGGWIDYARKMEQAGADGLELNVYMLATDPVRTSAEIEQVYIDVLKAV
                     KSSVKIPVAMKLSPFFSATAAMAKRLDDAGADALVLFNRFYQPDLDLEALEVKPGVVL
                     SSPEELRLPLRWIAILFGRIKASMAGTSGVHTATDALKLIMAGADVANLCAVLLKHGP
                     AHLGKILDEMEKWLEEHEYTNLRMARGSMSQKNVAEPAAYERANYIKALNSYTR"
     gene            complement(7227..10814)
                     /gene="nifJ"
                     /locus_tag="PKM23_00945"
     CDS             complement(7227..10814)
                     /gene="nifJ"
                     /locus_tag="PKM23_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNY90065.1"
                     /translation="MAREKILVDGNEATALVAHKINEVIAIYPITPSSNMGEWADAYS
                     AEGRKNIWGTVPQVVEMQSEGGAAGAVHGALQTGALTTTFTASQGLLLMIPNMFKIAG
                     ELTSTVFHVSARTVATHALSIFGDHSDIMAVRSAGWGLVASNSVQEAHDFALIAQAAT
                     LKARVPFVHFFDGFRTSHEVQKIEGLDEADLRAMIDDELVMAHRLRGLSPDRPVLRGT
                     AQNPDVFFQGREACNPFYAAVPEIVQEAMDKFAKVVGRQYHLFDYVGDPNAEHVLIVM
                     GSAAETAEETVEYLNKKGEKVGLIKVHLFRPFSTEHLLKALPKSVKQIAVLDRTKEPG
                     AGGEPLYMDVVTALTEAFSAGKLPFAQLPKVIGGRYGLSSKEFTPAMVVAVFNEMKKA
                     TPKNHFTVGINDDVSHTSLDFDPAFSIEPDDVFRALFYGLGSDGTVGANKNSIKIIGE
                     ETENYSQGYFVYDSKKAGTVTVSHLRFGPRPIKSIYLVDQAQFIGCHQQFIMERFNLL
                     EKAVTGGTFLLNSLSGPDEVWDTLPRVYQEEIVNKKMNFYVIDAYEVAKKTGMGVRIN
                     TIMQTCFFAISGVLPQEEAIRYIKKAIKKTYGAKGDAVVQKNYEAVDATLAHLHKVKV
                     PAVVTSTKEMPAVVPAHAPKYVRDVVSQIIIEKGDQIPVSALPVDGTFESATTQYEKR
                     NIALEIPIWDAEVCIQCGKCSMVCPHAAIRQKAYDPKHLDKAPATFKSTASRGKDFPE
                     GWKYTIQVAPEDCTGCALCVEVCPAKNKTEVGKKAINLGEQPPIRFQERENYEFFLNI
                     PDVDRREVKIASVKGSQLLRPLFEYSGACAGCGETPYLKLLTQLFGDRMVAANATGCS
                     SIYSGNLPTTPYAKNQDGRGPAWNNSLFEDNAEFGFGYRLTIDKHSEFARELVKLLAG
                     EIGEDLTTALLTADQSSEAGIHEQRERVALLKKKLAGMKSAQARHLHSLADMLVKKSV
                     WVIGGDGWAYDIGYGGLDHVLASGRNVNVLVLDTEVYSNTGGQMSKSTPLGAVAKFAA
                     AGKPLPKKDMGMIFMSYGNIYVARVAMGYNDLQVVRAFTEADAYDGPSIILAYSHCIA
                     HGIPMHKGMEEQKAAVESGHYPLFRFNPELAAQGQNPLKLDSKAPSITFEDFAYRETR
                     FKMLTKSHPERARQLMADATRSTRDRWHLYENWAAMEYGQGGNKAEAAE"
     gene            11159..11995
                     /locus_tag="PKM23_00950"
     CDS             11159..11995
                     /locus_tag="PKM23_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNY90066.1"
                     /translation="MNPSNALSRLAATEAVICDLDGTLYLDGLPLPGAHDFLRAVLDS
                     GRQLFYFTNNTSRSRRSYIEKLGRIRFPVCAEQIITAADCTFTYLRHHHLGPAIYLIG
                     NNDLKREFEEAGFACWDEERCSRELPQAVVLGFDTELTYAKIRTGYELMVKGVPYIAT
                     HADLLCPVAGGRFIPDVGSFISLFATATGGSEPLVMGKPNRPAVEAICDRTGLPAEKI
                     AFIGDRLYTDIRMAHKFGMVGVLVLSGETSREMAAASPDHPLMTVDSVADLIPHLERP
                     RL"
     gene            complement(12117..12710)
                     /gene="pgsA"
                     /locus_tag="PKM23_00955"
     CDS             complement(12117..12710)
                     /gene="pgsA"
                     /locus_tag="PKM23_00955"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNY90067.1"
                     /translation="MKLPNILTVSRIFLSPLFIIAFMFDTLPSYIICFFLALIIELSD
                     MFDGFFARRNKQTSDFGKLMDPFADSISRFSIFLCFLSADLAPLWVIAIFFYRDTLVS
                     IIRVFSVLEGFVVSARQSGKIKAWGQAVSIWLVLILIVVHKAEWWPWLAEVPGRYHML
                     TSIIIFIAALITLWSGIDYWNSHKKAVINAMKPKPTA"
     gene            12947..13324
                     /locus_tag="PKM23_00960"
     CDS             12947..13324
                     /locus_tag="PKM23_00960"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNY90068.1"
                     /translation="MDIKITFPGGLRVDAQYGPVTIPTNQDGSAPAPFALFLASIGTC
                     AGIYVLSFCKQRGLNTEGLEITQSMITDPVTHVIRKIALEIKLPAGFPEKYREAVIRA
                     ADQCAVKKHLAQPPEFSISTTGA"
     gene            13505..14545
                     /locus_tag="PKM23_00965"
     CDS             13505..14545
                     /locus_tag="PKM23_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNY90069.1"
                     /translation="MIHSSKNGMAEMVSRQFVEEIAEEDEEPPPDLLAECDSDETISA
                     VQRALAERHEVVLIESDEFAFHHLQESRPDLVFNMAERLSGPNRESQIPTLCEYLNIP
                     YTGSDPLTLGICLDKSRAKEILSYYQIPNPHFWIFDCEDAVPDGLPLPLMVKPLYEGS
                     SKGIKDNSLVRTSAELRERVGEVSRHYRQPVIVERFLPGREFTVGFIGNNPGLEILPV
                     VEIDHAQLPRGAAPIYSYEAKWIWDTTDKPLAIFKCPADISAELQAGIEDLVRKVVKV
                     LRLKDWGRIDIRLDEKDEPQIIEVNPLPGILPNPDDNSCLPKAARAAGYSYNQLILRV
                     VEEAAVRNGLAS"
     gene            14542..15552
                     /locus_tag="PKM23_00970"
     CDS             14542..15552
                     /locus_tag="PKM23_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNY90070.1"
                     /translation="MTGTSPLVGIAFNSFAPAANRSDETISETSVEQTAAELFAALRD
                     AGYAAVLLPLRDSLTDLVERLREQPCDLLINLCEAFRGIPQLEPHVAAFFEAMGWPFT
                     GNSPVTLALCQQKFVSKAILQAHGLPCPDGALVQPGAELPDIALPVIVKPNAEDASLG
                     IHPDSVIYERAQLQRQVERIWTGYHQPALVERFIDGREFNVGVLQRGSRLEALPVSEI
                     SFAGMPEGTPRIVGYEAKWFEDHPLYQATVPLCPAPITETLREALQSLAVKAFSAMGC
                     RDYARVDFRTSGEGEIFVLEVNPNPDIGLGAGYARALKAAGLPYTEFWRILINNALER
                     KE"
     gene            15555..16028
                     /locus_tag="PKM23_00975"
     CDS             15555..16028
                     /locus_tag="PKM23_00975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY90071.1"
                     /translation="MIRAMQPQDKEEVIRLLQATGMFTAEEVVVAEELVDLYLNLPGQ
                     KDYIVDVLEEDSGRVVGFATWGPTPLTDGTFDLYWIAVSPEAQGKGYGKMLLRQVENK
                     IEEEKGRVLIIETSSQPRYESTRQFYLKQHYQEVARIADFYRANDDRIIYAKYFS"
     gene            16045..17178
                     /locus_tag="PKM23_00980"
     CDS             16045..17178
                     /locus_tag="PKM23_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNY90072.1"
                     /translation="MEHWQQLLRGSLTKPEEIAKRFDLDVEEVKKVAEVFQFRITPYY
                     ASLIKEKGDPIYRQIVPDVEELAEKSGIADPLSEDKDSPVPSIVHRYPDRVLFLVSHS
                     CASYCRFCTRKRKVGNSDKINPRHIDAGIEYIRQHSEIRDVIVSGGDPLMLADSKLEY
                     ILSSLRSIKHIEIIRIGSRVPCFLPQRVTPELVNMLKKYHPLYMNVHFNHPDEITEES
                     SRALNLLADAGIPLGNQTVLLKGVNDNPETMKRLMQKLLTVRVRPYYIYQADYVQGTG
                     YLRTRVEKGLEIMQALRGWTSGLAVPAFVIDAPGGGGKIPLLPEYVQSVDDEKVVMRN
                     YKGELFVYPQESKAGSPSVPANAHIAPRCPALEAFPELEEIIC"
     gene            17183..18118
                     /locus_tag="PKM23_00985"
     CDS             17183..18118
                     /locus_tag="PKM23_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90073.1"
                     /translation="MIGKNRISHSAKPYYSPAPDLVILWEWEYDDDFVFYLAAAARQR
                     SLEVQIYAPAQLPAFLHTSSREDYAPRMVIDRASDVHPSLRPGLDALVKRGTQIINEP
                     ARVEWCRDKATMHLELVSSGINVPYGIIASRHDSPEGTFNLAMEKLGLPFVIKPSEGG
                     GGEGVILDAASPGHIAEALKKSPTGKVVLQKKVAPQLLSGRRAWFRVFYILDLITLCW
                     WDDLTHLYGPCAENDIPQGSLVQMQQIVAHIARTTQIHFFSTEIAIDHDGSLQVIDFV
                     NEICDMRMRSRHANGVPDALVMKIVNRLIDHCARL"
     gene            18234..20030
                     /locus_tag="PKM23_00990"
     CDS             18234..20030
                     /locus_tag="PKM23_00990"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNY90074.1"
                     /translation="MKGLKPALLLLLPLLLTWWSAGRAQITEAEITSQELLNHISFLA
                     SDTLKGRRSGSPEAERAAEYIRDQFAADGLVLLGKEGLQSFEVLNSLSAGEHNRLSIG
                     GEHAVPGRDFTPLAFSENGELAAAVHFAGYGFAIETDSLTWQDFAGQELKGKWALILR
                     DGPPPPASGQDRFEPHRSLRKKAQAARDHGAAGVLFVPAAAQEEESLPRLNADQGLGS
                     LGIPLFHIRRSLADRILAGSGVTLAGLQQQLDSTLTPASMALPCTVDARAEVVRQMGR
                     THNVVGLLPGSDPLLRDEYVIIGAHYDHLGMGGPGSGSRRPDTLAVHNGADDNASGVA
                     GLLELAEKWAAATEKPKRSLLFVAFAAEEMGLLGSKHFVDHPLVDLSRAQLMINMDMI
                     GRLNPATRAMTFGGTGTAAGLDTLVISLAAAHDLHPTLSPEGYGPSDHASFYARDIAV
                     LFVWTNISEEYHTPEDDAWRINAQGEQDVVSLVFDISREAADRSSRLAFQEAGPKSQP
                     ATSRRFKVTLGIMPDFTSTSGKGLRADAVMPDRPAARAGMKKGDIIIAMEGKPVGDIY
                     EYMNRLADFRVGQRISMEILRDGVKQILIVEL"
     gene            20094..20804
                     /locus_tag="PKM23_00995"
     CDS             20094..20804
                     /locus_tag="PKM23_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNY90075.1"
                     /translation="MRAFILVGGLGTRLRALGITQPKAMVDIGGRPFLEYLVRYLARH
                     NLREIVFCIGYGAEEIRSWFDDGSRWGVKILYSAEKSPLGTGGALKQAESFAVEENLV
                     CNGDSFLELELPDFANFHRKRRALASIAAINVHSMGDYGNIQTGRGGRINAFAEKSAG
                     GRGLINGGVYLLQREVWETIPGGGPLSLEREIFPRLAEGGRCYAFKTAGYFLDIGTPE
                     RLDRARAELPSVMQRILP"
     gene            20859..21866
                     /locus_tag="PKM23_01000"
     CDS             20859..21866
                     /locus_tag="PKM23_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNY90076.1"
                     /translation="MIIRAKAPLRISFCGGGTDVAPYPEERGGVVLSATIDKYAYASL
                     ITKEEARIRITSLDYDTEASYRLNEPIPFNGELDLAKAVVKHFAPRTGLHLFLHSDAP
                     PGSGLGSSSTMTVAMVGVFKQWQNHPLTDYEIAELTFRIERVDLQIVGGKQDQYAATF
                     GGFNFIEFFDKTVVVNPLRINDAVINELEYNLLLCYTGKTRLSANIVANQTRSYQERK
                     PAVVEALDQMKALTVEMKNCLLRGRLDDFGQLLHAAWLHKKNLDANISTSQIDQLYET
                     GRENGALGGKILGAGGGGYLLFYCPFQKKHRVAEALEKAGGQVVKFSFEQRGLQIWDI
                     R"
     gene            21863..22987
                     /locus_tag="PKM23_01005"
     CDS             21863..22987
                     /locus_tag="PKM23_01005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90077.1"
                     /translation="MKIVLVGTSFPMRGGIAHYVALLYKKLLDMGHEVEIISFKRQYP
                     DLLFPGKTQQDNSQQTIELPSRPLIDSINPLSWIRTFLYIRRTKPELVVYKYWMPFFA
                     PCYGTIVLLTRLFTRTKSLYIGDNIVPHEQIPVVDWLLTKWALWKVDYFIVQSQTVEN
                     DLLKIKPHARFRQVPHPVYEIFSSSYTRETARARLTLGEEEKVLLFFGYVRAYKGLHY
                     LIAALPELLREMEVRLLVAGEFYEDEEKYRLQIRELGLEQAILLKTDYIPNEEVGLYF
                     TAADVVVLPYVSATQSGIIQIAYNFDKPVITTDVGGLPEVIEPGRTGFVVPAEDSHAL
                     AQAILHFYRVREEADFPEFIKTYKKRFSWENLANAITGFV"
     gene            23039..24745
                     /locus_tag="PKM23_01010"
                     /pseudo
     CDS             23039..24745
                     /locus_tag="PKM23_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(24799..26568)
                     /locus_tag="PKM23_01015"
     CDS             complement(24799..26568)
                     /locus_tag="PKM23_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNY90078.1"
                     /translation="MMKRIALTFLLLGLAALPKSFAQPRTIPVTGITIHGARTLGEEE
                     VRAEFGLKRGDPFVAADLQLRGRRLLESLAAAGYWYARLDSIVYRVAADSSGAELRLY
                     LDEGRELRTGGVELAGLDSISTRRLVQRFTTRPGGRLDGAELEEDLDDALLQMDREGH
                     PFARLELQELRLDSLDSARDGLQLRYSAAPGPRLILREIQLAGNTLTRPGVILREIRI
                     KPGERYSQQKVERIVGRLMRLGYFKKVEEPILFYTSGDEGGLLLRVEEGQSSRFDGVV
                     GYSPASGDEKGYFTGLVDISLGNLLGTGRALSAHWQKKDRRTQDISLRYREPWLAGWP
                     LHIGGGFSQLIQDTTYVQRDLSLEVEIPLFDQLSIQAQAGRTEILPDSIGSYKFGLLR
                     SRTLNAAIGIEYDSRDDLLNPRQGVYYATTYQSGRKENLGPQPLLTGEVKRKTGTRRI
                     TLDLEFYTPLFQRQIVALGLHGRQFVSGETIVPVSDQFRLGGARTLRGFSEDQFRGSS
                     VAWLNCEYRYWLGRRSRTFLFADYGYYSAQRASGKLQEFKLGYGLGFRLETGLGIMSL
                     DYGLGRGDDLLGGKIHVGLINEF"
     gene            complement(26579..>29424)
                     /locus_tag="PKM23_01020"
     CDS             complement(26579..>29424)
                     /locus_tag="PKM23_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90079.1"
                     /translation="PIQPEGTTQNLQEIDKVFVQINSPHLSATMGDYTLDFTGSQFAQ
                     YSRKLQGAMGYAHFQNLQGTFSGAVSRGKYISQSITGQEGNQGPYQLKGDRGQIDMIV
                     LAGTERIYLDGQQMVRGETNDYIIDYASAQITFTRRRLITADSRIVADFQFSDEKYRR
                     SIYAARTAGSFWSGRLQLTGALIHESDDKDNPLDFTLDEDKLKILRAAGDNSALAAVE
                     GGSYVGPGRGRYVRSAEGIYTYVGADSGDYIVTFSDVGAGRGEYKYTGAGAYEYIGPG
                     AGRYKPVQLLTTAESHTLFDVQIAAEPYRALRLSGEMAMSSRDQNTWSGLGDDDNQGM
                     AQNWQLLVTPDTLRWGRRSLGLMELSGRFRQVQNRFRDIDRTSEAEYNRRWDLPDSAA
                     RGERVWEMAGRWAPFAGAQLAGEYGDIRKSGDFRSSRWQFEHGLTRNRWPDYSMRIER
                     ISRNNSPAREKGDWLRARGQAHYKIGAVSPLLEYEGEEKKENWSDSLATGFRFHSMSG
                     GLDLHPFKRVTARALYSWRDDQSYSGGGEFAGKSTARTQQLRLQAQQLGALSGSLEFT
                     HRARTYADSAVGRKETDLAELKLLLTPWKSALNADFNYQTSNTATAKKERLYIKVNSG
                     DGSYRFDENLREYVLDPLGDYILRILTTDELVPVVELKTSGRLRLDPSRFWNRTKGGK
                     PLRTWQKWLSAVSTETFSAIEERTQEKEVWQIYLLNLDKYQQPAVTLFGSMQWRQDIF
                     LFEKRRDFSLRLRYQQKQEMNNQYLEGGQERSDDEVSARLTGRIGARLISQSDFARKR
                     IARSFSESSRQDRDILSDQGRVEFSYRPKPALEFALEGSLAMEEDRCFAPATRVNYLS
                     LRPRVTYSWRSNGRWQAEVEWNRVAVAPEGRIIPYEMADGRSAGYSMRWDARFEYRLS
                     QTVQASFSYSGRNEPERQGVTHTGRAQITAAFR"
BASE COUNT         6012 a         9250 c         8261 g         5901 t
ORIGIN      
        1 accccggggg attaaaccgt tgcacttgat gctaatcatt cgtaaatttt atgtaattac
       61 agcttttcat cccgaaagga aaccagcgtt gcaagccatt ctcttcgatc ttgacggcac
      121 ccttgtccat accgcacccg acgttcatcg cgctatcaac tatgcccggg gcgtgcttgg
      181 cctgccgccc ctttcactcg cccaggccct caaggccatc ggcccgggct ccgaccgttt
      241 cgcccagacc gtcctcggcg acgaatatgc ccaattgctc gaggaatacc tcgaggtttt
      301 ccgcccctat tacctgcaaa tctgcgccga aaacagccgc cctttccccg gcatcgtcga
      361 actcctcgac gccctcaagg agtatcgcct cgcggtggtg accaacaaac ggctggtgca
      421 gagccgcgcc ctgctcgagg ccatcgatct tctgccctat ttcgaactcc tggttggacc
      481 cgaactggtc gaccacatca agcctgctcc ggacatgatc cactatgccc tcgaccagct
      541 cggcctcgcc cccgatcagg ccatcatggt cggcgacacc gacaacgatc tcctcgccgc
      601 ccgggccgca ggggtcaaaa actgcgcggt cacctggggg tattccgaca tcgcctttat
      661 acacaccctc aaaccggatc acctcatcga taaacccgaa gagctgctgc ccctcctggc
      721 cagctacaac caccaaccgc ggaaatagaa cgaagatgaa catcctcggc ctatcctgtt
      781 tctatcatga cagcgccgca gccctcatcc aggacggcga aatcctcgcc gccgcccagg
      841 aggagcgctt ttcgcgcatt aaacacgatc atgattttcc ccgccaggcc atcgcctcct
      901 gcctggagca ggccggaatg accgttgcaa atattgatta catcgtattt tacgagaaac
      961 ccctgctcaa attcgagcgc atcctcgaaa cctatctcag ttatgccccg ctcggcctgc
     1021 gcagctttct catggccatg cccctctggc tcaagaaaaa gctctggatc cccgacctta
     1081 tccgcaagga actcggcttc cagggcgaga tcctcttcac ggagcaccac caggcccacg
     1141 cggccgctgc ctttttcccc tcccccttct ccgaggcggc catcctgacc atggatggcg
     1201 tcggtgaatg ggccaccacc tcgtggggca gcggccgcac caaccacctc gacctccagg
     1261 ccgaaatcca ttttccccac tcactcggac tgctctactc ggcctttacc tattacaccg
     1321 gcttcaaggt caactcgggc gaatacaagg tgatgggact cgccccctat ggcgaacccc
     1381 gctacgtcca gaccatcctc gacaatctga tcgatctcag ggaggacggc tccttccgcc
     1441 tcaacatgaa gtggttcaat tacggggccg gactcaccat gaccgaccgc cgtttccaca
     1501 aactctttgg cggcccgccg cgcaaaccag agagcgccct gacccagcgg gagatggacc
     1561 tcgcccgctc tgtacaggag gtgaccgaga tggtcatgct gcgcgccgcc gcccatgtcc
     1621 atattcagac cgggagcaaa aatctctgtc tggccggggg cgtagccctc aactgcgtcg
     1681 gcaacggccg gatcctgcgc gaggggccct ttaaaaaaat ctggatccag cccgccgcag
     1741 gagatgccgg cggcgccctc ggggccgccc tcttcgcctg gtaccagatc ctcggcaatc
     1801 cgcggccgcc gcgcgggatt cacgaccacc agaagggttc cctgctcggc cctgaattca
     1861 gcgaagagga gatccgcgcc tttctggagg aaaaaaaact ccccttccgc aagatgacca
     1921 ctgcacagct gctcgatcgg gtctccgatc tcattgccac cggccaggtc atcggatggt
     1981 tccagggacg catggagttc gggccgcgcg cactcggcag ccgctcgatc atcggcgacg
     2041 cccgctcgcc tgaaatgcag tcgcgcatga atgtcaaaat caagttccgc gagagctttc
     2101 gccctttcgc cccggccgtg ctcgaggagg aggccgcaga gtggttcgag atcgaccact
     2161 ccagccccta tatgctcctg acggccccgg tccgccgcga gaaacgccgc gccatgacca
     2221 cagcagaaaa aaaactttgg ggcatcgaca agctcaaggt catccgctct gaaatccccg
     2281 ccatcaccca tgtcgattac tccgcccgcc tccagaccat ctcagcccgc gaacacggcc
     2341 tgttttatcg cctggtgaag cacttttttc aaaagaccgg ttgcccggtg atcatcaaca
     2401 cctccttcaa tgtgcggggg gaaccgatcg tttgcacccc cgaggaggcc tggcgatgct
     2461 tcatgcgcac tggcatggat gttctggtgt tgggccacta cctgctggac aagcaggaac
     2521 aacccccgct gagcggggat tcggactgga gaaaaaccta tgaactggat taaggatatc
     2581 cgcgacagcc tgcggcagct gccccgggat gagaaggcgc tgcagcgctt tgccgcgctt
     2641 ctcacctccc tgctcctcct ggccgcggca tggcttttct tcatccggcg ggacgccgct
     2701 ttaaccctct ggttcgtcct cgccaccctg cttctcatcg ccctggcgct gctccgcccc
     2761 cgcctgctcc gcccgctcca taccggctgg atggccctgg cctttgccct cggcgccctg
     2821 gtctcccgca tgcttcttgc cctcttcttt tatctcatcc tcacccctat cgcccttctg
     2881 ctcaggctgc tcggcaaaga cctgctgcaa aggaagttaa aaaaagaggg cgccagctac
     2941 tggatccggc gccctgtaaa agaggtaatc aaagaggact atcagcggct cttttaggcc
     3001 gtcagcccct cccaggtgag attgttgtcc gcgtcagcca ggcggggacg caccttgtct
     3061 ttctccgaca gaatcagctt tcccggcagg atcggccatg caataccgat cgacggatca
     3121 ttccagatga ttccccgatc gacctccggc gtgtaaaaat cggtgcactt gtacatgaac
     3181 tgggcccgtt cgctttgaac atagaagcca tgggcgaatc cgggcggaat gtacagggcc
     3241 ttcttgttct cctcggacag ttccacccct acccagcgtc cgtaaaaggg cgatccgcgc
     3301 cggatgtcca cggccacatc gaaaacacac ccttgtgtga cacgtaccag cttgccctgg
     3361 gcgtggggat ggagctggta atgcagtccg cgcagcgtgc cctgaaccga actgctgata
     3421 ttgtcctgga tgaacgggac cgtcagcccc agttcatgaa aagcccgctc attatagctc
     3481 tcgatgaaat aaccgcgcgc atcggcaaag acatcgtatt cgatcagcag cagatcgggg
     3541 atggcacagg gtacaaattt cacaaggggt ctccattctg gataattccg ctctcttctc
     3601 tctcatcatc gctgcgcatc gccccgcttc atccggccgg gttatttgac cctgccgtcg
     3661 catttggact caggagatac agatgctggg tgggggtatt gagccgggcc tttacctgaa
     3721 tatgatattt tgtaaaaagg tcggccagta tttcgtcctc gtggtaatat acgggtgatc
     3781 cgagcagcct gcggaaacgg gcgtgccagc ccgccgggga gacggtcagc agcagatgcc
     3841 cttcgcgggt gagcaggcgc gccgcttcgc ggagaagggg ctccggatcc cgccagtatt
     3901 cagccacccc gattaccagg aggagtccga aatgatgcgc ccgcagcggc aaggcggcca
     3961 gatcggcgca cagggcccac cccacctcac catagcggct cgcgcgcacc atccgacgcg
     4021 aacggtccac cccgagacag ggctcgtacc gcagcctggc gggcgccgat cctcttccgc
     4081 agccgagatc ggccatccag gactgcgagg agggaaccat ggcgagcagg cgggcagccg
     4141 cctcgtcttc cctgcgggta atgaaataca ggggccaaag actccggccc caccccagca
     4201 ggcgcagcct ctcgtcgcgg gcgccctgaa gaaagcgcac aagtctatta atggctgaga
     4261 aagagatcaa cgtgggcgaa cttttccaca tcgaccagac cgcggtccgt gagcttcagc
     4321 cgcggaatga cgggcagcgc catgaacgac agggtcatga gcggatcctc cagcgcgatg
     4381 cccagtgacc ggcaggctgc gatgagatcg cgggtgcggc tgctgacgaa ctctatcggc
     4441 ttgttggaca tcagcccggc gatgggcagc tcgagcgctt cgagtaccgt gctgttgtcg
     4501 actacggcaa tgccgcctcc catcttgttg atggtcgtga ccgctttcat gatgtcgcgg
     4561 tcgctgatgc ctgcggcaat aatgttgtgg ctgtcatgac cgatcgagga ggcgatggcc
     4621 ccccttttga ggccgaaacc cgtcatcagc cccttgccga tgtttcccga ggcccgatgg
     4681 cgctccagca cataaaggcg caccagatcc cgctcgggat cggagatgac atggccgtca
     4741 cgaaccgagg gctccatttc aaagaactgg gtgacgatct ggtcggggat gaccccgata
     4801 acccggcagc gcttgcccgt caccggtatt gaaaattcat caccctccag ccacttgatg
     4861 ttcacggagc tgcgcagcat cgccggcttg cgcaccggga cctcgtagac cggcaccccg
     4921 tcctgggcga ccagggcacc gtttttgtag accttgtgaa tatggaagga atcaaaatcg
     4981 tcaaacacca ccatatccgc caattttccc ggtccgatcc ccccaagctt gcgcagcccg
     5041 aagtactcgg ccgtattgag ggtggccatg cgcaccgcag tgaccgggtt gaggccggcc
     5101 ttgatcgcac ttttgatgat gtaattgaca tgcccctcgc ggaggatatc gtcgggatgg
     5161 cggtcgtccg tgcagaagaa gcagcgccgc tcattttccg gggtgaccag gggaaggagg
     5221 tcgtgaaggt tcttggtgcc cgagccctcg cggatcatga tgtacatgcc gagggccagt
     5281 ttctcccgcg cctcctccac agtggtgcat tcatgatccg attccacccc ggcggcgata
     5341 taggcggcca gatccgcgcc gctcagcccc ggggcatggc cgtcgatgcg tttgtcggtc
     5401 acgatcttga tcttgtcgag cacatcgggc tcggcgttga ggacgccggg atagttcatc
     5461 atctcaccca gacccagaac ccagcgctcg cgcagaaagg gaaaaatgtc aaaggcgcgc
     5521 agctctgagc ccgctgattc caggggcgag gagggcacac aggaggagag catgaagaag
     5581 acattgagag ggttgtattt gctgacatcc atcatataac ggatgccctc atagccgagc
     5641 acattggcga tttcgtgggg atcggcgatg accgaggtgg tgccgagggg gacgaccgcc
     5701 cggccgaact gcggaacctc caccatcgag ctctcgatat ggacatggcc gtcgatgaat
     5761 ccgggggcaa gatgcatccc cttgagatcc acctcctcct cgccgcgata gccgctgccc
     5821 agcccgacga tcgtatcccg gtagaccgcc acatcgctca ggacgatctc accggaatag
     5881 acattgatga ctcgcgcatt gcgcagcacc agtgagcact tgatctggtg gcgcgcggcc
     5941 tggatcagct ctttgaccat gacacctccc cgacgaatga cgggttcagc ccgaccggca
     6001 attctttttc tataatagca atcatcctga aaaaaatcaa ggctaaatcg cacgatcttc
     6061 catcgcccgc acaaaaaaaa gaaaggccgg tcccctgcaa ggagaaccgg ccttccggtc
     6121 agatcagacc agcccctcag cgggtgtaac tgttgagcgc cttgatatag ttggcgcgct
     6181 cgtaggcagc gggttcggcc acatttttct ggctcatgct tccgcgcgcc atcctgagat
     6241 tggtgtattc gtgctcttcg agccattttt ccatctcgtc gaggatcttg ccgaggtggg
     6301 cgggcccgtg cttgagcaga acggcgcaca gattggccac atcggccccc gccatgatca
     6361 gcttgagggc atcagtggcg gtatggactc cggaggtccc ggccatgctc gccttgatgc
     6421 ggccgaagag gatggcgatc cagcgtagag gcaggcgcag ctcctcgggg ctgctcaaca
     6481 ccaccccggg cttgacctca agggcttcca gatcgagatc gggctggtaa aaacggttga
     6541 aaaggaccag agcatccgcg cctgcgtcat cgagccgctt ggccatcgct gcagtcgcgc
     6601 tgaaaaaggg gctcagcttc atggcaacag gaatcttgac gctgctcttg acggccttga
     6661 ggacgtcgat gtagacctgt tcaatctcgg cgctggtgcg gactggatcg gtggcaagca
     6721 tgtagacgtt gagctcgagt ccatcagcgc ccgcctgctc catcttgcgg gcatagtcga
     6781 tccagccacc agtggagacc ccgttgaggc tggcgataat cgggattccg gtcgcctcct
     6841 tggccttgcg gatgtgctca agatagccct caggcccaag ccgatactcc tgcgcctcgg
     6901 gaaaataggt cagcgcctcg gcaaaactgt cggtgccata gctgaggaaa tgatccagct
     6961 cctcggcctc gaacgagatc tgctcttcga aaagggaata gagaacgatc gccgatgccc
     7021 cggcatcctc gagattcttg atggtatcca gattcttgga gaggggggag gccgaaggaa
     7081 ccagcgggtt cttgagcttc aagcccatat aggtggttgt taaatccata tatgcctgtc
     7141 ctttctatct cggtgagcgc ctcggcggat tcaggggtga atccgccggg cggattagtc
     7201 tcgaatgaac cacgggatga gagcgattac tccgccgctt cggccttgtt tccgccctgg
     7261 ccatattcca tcgccgccca gttctcgtaa aggtgccaac gatcacgggt cgaccgggtg
     7321 gcatcagcca tcagctgccg ggcgcgttcg ggatggctct tggtcagcat cttgaagcgg
     7381 gtctcgcgat aggcaaaatc ctcaaaggtt atcgagggcg ccttggagtc cagcttgagg
     7441 gggttctgac cctgcgctgc cagctccgga ttgaagcgga agaggggata atggcccgat
     7501 tcaaccgcgg ccttctgttc ctccatcccc ttgtgcatcg ggatgccgtg ggcgatgcag
     7561 tgcgagtagg ccagaatgat gctcggcccg tcataggcgt cggcctcggt gaaggcacgc
     7621 acgacctgaa gatcgttgta gcccatggcc acgcgggcga cgtagatgtt gccatagctc
     7681 atgaagatca tgcccatgtc cttcttgggc aggggcttac cggcggccgc gaatttggcc
     7741 accgcaccga gcggagtgga cttggacatc tggccgccgg tgttggagta gacctcggtg
     7801 tcgagcacga gcacgttgac attgcggcct gaggcgagga cgtggtccag gccgccgtag
     7861 ccgatgtcgt aggcccagcc gtcaccgccg atgacccaga cgctcttttt gaccagcata
     7921 tcagcgaggg aatggagatg ccgggcctgc gccgatttca tgccggcgag ctttttcttc
     7981 aggagtgcga cccgctcacg ctgttcatgg atgcccgcct cgctgctctg atcggccgtc
     8041 agcagtgcag tggtcagatc ctcaccgatc tcgccggcga gaagtttgac cagttcccgg
     8101 gcgaactcgc tgtgtttgtc aatggtcagc cggtaaccga agccgaattc cgcgttatcc
     8161 tcaaagaggg agttgttcca tgccggcccg cgcccatcct ggttcttggc atagggggtc
     8221 gtcggcaggt tgccgctgta gatcgaggag cagcccgtgg cgttggccgc caccatgcgg
     8281 tcgccgaaga gctgggtcag cagcttgagg tagggagtct caccgcagcc ggcgcaggcg
     8341 cccgagtact cgaagagcgg ccgcagcagc tggctgccct tcaccgaggc gatcttgacc
     8401 tcccggcggt ctacgtcagg gatattgagg aagaactcgt aattctcgcg ctcctgaaaa
     8461 cgaatcgggg gctgttcacc caagttgatc gcctttttgc cgacctcggt cttgtttttg
     8521 gccgggcaga cctcgacgca gagcgcacag ccggtgcagt cctctggcgc cacctggatg
     8581 gtgtacttcc agccttccgg aaaatccttg ccgcgggaag ccgtcgattt gaaagtggcc
     8641 ggcgccttgt ccagatgctt gggatcgtag gccttctggc ggatggccgc gtggggacaa
     8701 accatggagc acttgccgca ctggatacag acctccgcat cccagatcgg gatctccagg
     8761 gcgatgttgc gcttctcgta ctgggtcgtc gcggactcga aggtgccgtc gaccggcagg
     8821 gcgctcaccg gaatctgatc gcccttttcg atgatgatct ggctgaccac atcgcgcaca
     8881 tacttgggag catgggccgg aaccaccgcc ggcatctcct tggtgctggt caccacagcg
     8941 gggaccttga ccttgtggag atgggccagg gtggcatcca ccgcttcgta gttcttctgc
     9001 accacggcat cgcccttggc gccataggtc ttcttgatcg ccttcttgat gtagcggatc
     9061 gcctcctcct ggggcagaac cccggagatg gcgaaaaaac aggtctgcat aatggtgttg
     9121 atgcgcacgc ccatgccggt tttcttggcc acttcgtagg cgtcgatgac gtaaaaattc
     9181 atctttttgt tgacgatctc ttcctgatag acccgcggca gggtgtccca gacttcgtcg
     9241 ggaccgctga ggctgttcag caggaaggtc ccacctgtca cggccttctc gagcagatta
     9301 aagcgctcca tgatgaactg ctgatggcag ccaatgaact gcgcctggtc gacgagatag
     9361 atcgacttga tcgggcgggg gccgaaacga agatgcgaca ctgtcaccgt gcccgctttt
     9421 ttcgaatcgt agacaaagta gccctgggag taattttcgg tctcttcacc gatgattttg
     9481 atcgagttct tgttggcgcc gaccgtgccg tccgagccaa ggccgtaaaa aagggcgcgg
     9541 aaaacatcgt cgggctcgat cgaaaaggcg ggatcgaaat cgagactggt gtgggaaaca
     9601 tcgtcgttga tgccgacggt gaagtgattt ttgggggttg cctttttcat ctcgttaaag
     9661 accgccacga ccatggccgg agtgaactct ttggaggaaa ggccatagcg gccgccgatc
     9721 accttgggca gctgggcaaa gggaagcttt ccggccgaaa aggcctcggt cagggccgtg
     9781 accacatcca tgtagagggg ctcgccgccg gcgccaggtt ccttggtccg gtccagcacc
     9841 gcaatctgct tgacgctctt gggcagggcc ttgagcaggt gctcggtcga gaagggacgg
     9901 aaaagatgga ccttgatgag gccgaccttt tcgccctttt tgttgaggta ttcgacggtc
     9961 tcttcggcgg tctcggcggc cgaacccatg acgataagca cgtgttcggc attcgggtcg
    10021 cccacatagt cgaagagatg atattggcgg ccgaccacct tggcgaattt gtccatcgcc
    10081 tcctggacga tctccgggac ggcagcatag aagggattgc aggcctcgcg gccctggaaa
    10141 aagacgtccg gattctgggc agtgccgcgc agcaccggcc gatccggcga aaggccgcgc
    10201 agacgatggg ccattaccag ctcatcatcg atcatggcgc gcaggtcggc ctcgtccagt
    10261 ccctcgatct tttgcacctc gtgcgaggtg cggaagccgt caaaaaagtg aacgaaagga
    10321 acccgcgcct tcaaggtggc ggcctgggcg atcagggcaa agtcatgggc ctcctgcacc
    10381 gaattggagg cgaccagccc ccaacccgcg gagcgcaccg ccatgatgtc gctgtggtcg
    10441 ccgaaaatcg acagggcatg ggtggccacc gtacgggcgg agacgtggaa tactgtggag
    10501 gtcagttcgc cggcaatctt gaacatgttc gggatcatca gaagcaagcc ctgagaggcg
    10561 gtgaaggttg tcgtcagtgc ccccgtctgc agggcgccgt gcacggcgcc ggccgcgccg
    10621 ccctcgctct gcatctcaac cacctgaggc acagtgcccc agatattctt ccggccttcg
    10681 gcggaatagg catccgccca ctcacccatg ttggatgagg gggtgatggg atagatcgcg
    10741 atcacctcgt tgatcttgtg ggcaacgagg gctgtcgctt catttccgtc gaccaggatc
    10801 ttttctcttg ccattacatg cctccataca gatgttgatt atgcgatatg ggactcaaat
    10861 cagttgttga ccggaagtac ttttccgctc agtgccggcc gcggcgatcg ggcggatcga
    10921 acgagttgcc tgtaatcagc aactaagtta aataaaaaat aagtggaaag caagaatttt
    10981 agcggagaaa gacataacgg tgggaaccag atttcccggg gtacgcggag caaggcataa
    11041 cagtggcaac cagatttcgg ggggtaccct ccaggagatt gaccttgatt ttgttgctta
    11101 ttttccttta attggagcca gtcagcccct tccacccctt gatgcgagtg attgactcat
    11161 gaatccatcc aacgctctct cccggctggc cgccactgag gcggtcatct gcgacctcga
    11221 cggcacgctc tatctcgacg gcctccccct gcccggcgcc cacgatttcc tgcgcgcggt
    11281 cctggactcc ggccgccagc tcttctattt caccaacaat acctcccgct cccgccgcag
    11341 ttatatcgaa aaactgggcc ggatccggtt cccggtctgc gcggaacaga tcatcaccgc
    11401 cgccgactgc accttcacct accttcgtca tcatcacctc ggccccgcta tctacctgat
    11461 cggcaacaat gacctcaagc gggaattcga agaggcgggc tttgcctgct gggatgaaga
    11521 gcgctgctca cgtgaactcc cgcaggcggt ggtgctcggc ttcgacaccg aattgaccta
    11581 tgcgaagatc cgcaccggtt atgagctgat ggtgaagggc gtcccctaca tcgccaccca
    11641 tgccgacctc ctctgtcctg tcgccggcgg ccggttcatc ccggatgtgg gctccttcat
    11701 ttccctcttt gccaccgcca ccggcggttc ggaacccctg gtcatgggca agcccaaccg
    11761 gccggctgtc gaggccattt gtgaccgcac cgggctgccg gccgaaaaaa tcgccttcat
    11821 cggcgaccgg ctctacaccg acatacgcat ggctcacaaa ttcgggatgg tcggtgtcct
    11881 ggtcctcagt ggcgaaacca gccgtgagat ggctgcggcc tccccggatc accccctcat
    11941 gaccgtcgat tctgtcgccg atcttatccc tcacctggag cgcccccgcc tctaggcctg
    12001 cgcaccccca aatagcaaac cccggctcga atattcaggc cggggttatt tttatttcat
    12061 ctgctgacag gcgcgttacg cctcacacag aaaaatccgc tcgccgccgc ctggaatcag
    12121 gcggtcggtt tgggcttcat cgcattgatg accgcctttt tgtgcgagtt ccagtaatcg
    12181 atgccggacc aaagggtgat cagcgccgcg ataaagatga tgatcgaggt gagcatatga
    12241 tagcgtccgg gcacttcagc aagccagggc caccactccg ccttgtggac gacgatgagg
    12301 atgagcacca gccagatgct gaccgcctgg ccccaggcct tgatctttcc gctctgccgc
    12361 gccgaaacca caaaaccctc cagcaccgaa aaaacgcgga tgatcgaaac cagggtgtcg
    12421 cgatagaaaa agatggcgat cacccagagg ggggccaaat cagcactcag gaaacaaaga
    12481 aaaatagaga aacgcgagat gctgtcggca aagggatcca tcagcttgcc gaaatcggag
    12541 gtctgtttgt tgcgccgcgc aaaaaagccg tcaaacatat ccgacagctc aatgatcagg
    12601 gcgagaaaga aacagatgat gtatgagggc agggtatcga acatgaaggc gatgatgaac
    12661 agcggggata aaaaaatccg gctgacggtg aggatattgg gcagtttcat gggctctctt
    12721 tgtcgattat tatttgctga ttttgccata aaataaacaa aaaaagacca agcatcaaga
    12781 tattttatct cagggtaccc ccctaattcc gactacctga gcaaatcaac gcgcatgaag
    12841 ccgaaagcct gaatccaacc cggacccccg tcatctaaca gcatggttgt ttaatccaca
    12901 aaccctgccg gccagtggcc ggcccgatct ctttaaggag gcagcaatgg atatcaaaat
    12961 cacctttccg ggcggactgc gtgtggacgc ccaatacggt cccgtgacca tccctactaa
    13021 tcaggacggc tcagcgcccg cccccttcgc ccttttcctc gcttccatcg gcacctgcgc
    13081 gggcatctat gtgctcagct tttgcaaaca acgcggcctg aacaccgaag ggctggagat
    13141 cacccagagc atgatcaccg accctgtcac ccatgtgatc cgcaagatcg ccctggagat
    13201 caagctgccc gccggatttc ctgaaaaata ccgtgaagcg gtgatccggg cggcggatca
    13261 gtgtgcggtg aaaaaacatc tcgcgcagcc cccggaattt tccatctcga cgacaggggc
    13321 ttgagggaag acccaatata ttgacgccgc gcaaaaaaga ataaataagt attgactttt
    13381 atgcagaaaa ggctatattt atccccaaat tattcatgca attattttac ctgatacgaa
    13441 actttttcgg cccaacgtcg ttatattctg aaattcagga gagggcatca tcttgaaaat
    13501 tgctttgata catagctcta aaaacggcat ggccgagatg gtttcgcgtc aattcgtcga
    13561 ggaaatagcc gaggaggatg aagaacctcc tccagatttg ctcgccgagt gtgattccga
    13621 cgagaccata tcagccgttc agcgcgccct cgctgaacgg catgaggttg ttttaatcga
    13681 atccgatgaa ttcgctttcc atcacctcca ggaaagccgc cctgaccttg tgtttaatat
    13741 ggccgaacgc ctttccggcc ccaaccgtga atcccaaatc ccaactcttt gcgaatacct
    13801 caacattccc tacaccggct ccgatcccct caccctgggc atctgccttg acaagtcacg
    13861 cgccaaggaa atcctctcct actatcagat tcccaatccg cacttctgga tattcgattg
    13921 cgaggatgct gttcccgatg gcctgcctct gcccctgatg gtcaaacccc tctatgaggg
    13981 ctcaagcaag gggatcaagg acaactccct ggtccgcacc tccgccgaac ttagggaacg
    14041 ggtcggcgag gtcagccgcc actaccgcca gccggtcatc gtcgaacgct ttctacccgg
    14101 ccgcgaattc acggtcggct ttatcggcaa taatcccggc ctcgagattc tccccgttgt
    14161 cgaaatcgac catgctcaac tcccccgggg ggcggcgcca atctattcct atgaagcgaa
    14221 atggatctgg gacacaaccg acaaacccct cgccatcttc aagtgccctg ccgatatcag
    14281 cgcggagctc caggccggga tagaagacct ggtccgcaag gtcgtcaagg ttctccgcct
    14341 caaggattgg ggccgcatcg atatccgtct cgatgaaaag gacgaaccgc aaatcattga
    14401 agtcaatcca ctcccgggga ttctgcctaa tccggatgac aattcatgtt tgcctaaagc
    14461 ggcacgcgct gccggttaca gctacaacca gctgatcctt cgtgtggttg aggaggctgc
    14521 cgtgcgcaat ggactggcat catgaccgga accagcccgc tcgtgggcat tgcgttcaac
    14581 tctttcgcgc ccgctgccaa ccgcagcgat gagaccatct ctgaaacctc cgtcgagcaa
    14641 acagccgcgg agctcttcgc cgcactgcgc gatgccggct atgcagcagt gctgctgccg
    14701 ctgcgcgaca gtctcaccga tctggtggag cgactccgcg aacaaccctg cgatctgctc
    14761 atcaatctat gcgaggcctt tcgcggcatt ccgcagctcg aaccacatgt ggccgccttt
    14821 tttgaggcca tgggctggcc cttcaccggc aacagtccgg tcacactggc cctctgtcag
    14881 cagaaattcg tgagcaaagc catcctgcag gcgcacgggc tgccctgccc tgacggtgca
    14941 cttgttcagc ccggcgctga gctgcccgat atcgccctgc cggtcatcgt taaaccgaat
    15001 gcggaggatg ccagcctggg catccatcct gattcggtga tctacgagcg ggcccaactg
    15061 cagcggcagg tcgagagaat ctggaccggc taccatcaac ccgccctggt cgaacgcttc
    15121 atcgacggcc gcgagttcaa tgtcggtgtc ctccagagag ggagccgcct ggaagccttg
    15181 ccggtctccg agatcagctt tgccggcatg cccgagggga ccccccgaat tgtcggctat
    15241 gaggcaaaat ggtttgagga ccatcccctc taccaggcca ccgttcccct ctgcccggcc
    15301 ccgatcacgg aaaccctgcg ggaggccttg caaagcctgg cggtcaaggc cttttcggcg
    15361 atgggctgcc gcgactatgc ccgggtcgat ttccggacca gcggtgaggg cgagatcttt
    15421 gtcctcgagg tcaatcccaa tcccgatatc gggcttggcg ccggctatgc ccgtgccctc
    15481 aaggccgcag ggctgcctta taccgaattc tggcgcatcc tcatcaacaa cgccctcgag
    15541 cgcaaggagt gaccatgatc cgcgccatgc agccgcagga caaagaggaa gtgatacgcc
    15601 tgctccaagc aaccggcatg ttcaccgccg aagaagttgt ggtcgctgaa gaacttgtcg
    15661 acctttatct gaatctgccc gggcaaaagg attatatcgt ggatgtgctc gaggaagatt
    15721 caggccgggt tgtcggcttt gccacatggg ggcctacccc cctgaccgat ggcacttttg
    15781 atctctactg gattgccgtc tcacccgagg cgcaaggcaa ggggtacggg aaaatgctgc
    15841 tgcgccaggt tgagaataaa attgaggagg agaagggaag agtactgatc atcgagactt
    15901 cgtcccagcc gcggtacgaa agcacccggc aattctatct taaacagcac tatcaggaag
    15961 tcgcgcgcat cgccgatttt taccgagcga acgatgatcg cattatctat gccaagtatt
    16021 tctcttgaaa ggattatgaa cactatggaa cactggcagc aactcctcag gggcagtctc
    16081 accaagcccg aagagattgc caaacgcttc gacctggatg tcgaagaggt taaaaaggtt
    16141 gcagaggttt ttcagtttcg cattacgcca tattatgcca gcctgatcaa ggagaagggc
    16201 gatcccatct accgccagat tgtacccgat gttgaggagc tggccgaaaa gagcggcatc
    16261 gccgatcctc tttcggaaga caaggactcc cccgtgccga gcattgtcca tcgctatccg
    16321 gaccgtgttc tctttctggt atcgcacagc tgcgcctcct actgccgttt ctgcaccagg
    16381 aaacgcaagg tgggcaattc cgacaagatc aatcctcgcc atattgatgc cggcatcgaa
    16441 tatatccgcc agcacagtga aatccgcgac gtcatcgtct ccggcggcga cccgctcatg
    16501 ctcgctgaca gcaagctaga atatattttg tcttctttgc gttctattaa gcatatagag
    16561 atcatccgca tcggttcgcg cgtgccctgt tttctgccgc agcgcgtcac cccggagctg
    16621 gtgaacatgc tgaaaaagta ccaccccctc tacatgaatg ttcactttaa ccatccggat
    16681 gagatcacgg aagagtcttc gcgcgccctg aacctgcttg cagatgcggg cattcccctc
    16741 ggcaaccaga cggttctgct caagggcgtc aacgacaatc ccgagaccat gaagcgcctg
    16801 atgcagaaac tcctgaccgt acgcgtacgg ccctactaca tctatcaggc cgactacgtc
    16861 caggggaccg gctacctgcg cacccgcgtg gaaaaggggc tggaaatcat gcaggccttg
    16921 cgtggatgga cttcgggact ggccgtgccc gctttcgtca tcgacgcccc cggaggcgga
    16981 ggaaaaatcc ccctgctgcc cgaatatgtc cagtccgttg atgacgagaa ggtggtcatg
    17041 cgcaactaca agggcgaact tttcgtctac ccccaggagt cgaaggcagg cagcccatcc
    17101 gtgcccgcca atgctcacat tgcgccccgc tgcccggccc tggaggcatt tcctgaactg
    17161 gaagaaatca tctgctagga acgtgatcgg aaagaacaga atctcacaca gcgccaagcc
    17221 ctactattcc ccagctccgg atctggtcat tctctgggag tgggaatacg atgacgattt
    17281 tgtcttttat ctggccgccg ctgcaagaca gcgcagcctg gaggtgcaga tctatgcgcc
    17341 ggcccagctg cccgcctttc tgcataccag ctcacgcgaa gattacgccc ccaggatggt
    17401 gatcgaccgc gcctctgatg tccatccctc cctgcgtccg ggactggatg ccctcgtcaa
    17461 acgaggcacg cagatcatca atgagccggc ccgcgtggag tggtgccgcg acaaggcgac
    17521 catgcatctg gaactggttt ccagcggcat caacgtcccc tacggcatca tcgcctcgcg
    17581 tcatgactcc cctgaaggga ccttcaacct cgccatggaa aagctgggat tgcctttcgt
    17641 gatcaaaccc tccgaagggg gcggtggcga aggggtaatc ctcgacgccg cctcacccgg
    17701 ccatatcgcc gaagccctga aaaaaagccc gaccggcaag gttgtcctgc agaaaaaagt
    17761 ggccccgcag ctgctcagcg gccgccgggc ctggttcagg gtcttttaca tcctcgatct
    17821 catcaccctc tgctggtggg acgacctcac ccatctttat ggaccctgcg ccgaaaatga
    17881 tatcccgcag gggagcctgg tgcagatgca gcagatcgtc gcccacatcg cccgcacgac
    17941 gcagatccat ttcttctcga cggaaattgc catcgaccat gacggctccc tgcaggtcat
    18001 tgatttcgtc aacgagatct gcgacatgcg catgcgctca cgccatgcca acggcgtgcc
    18061 cgacgccctg gtcatgaaga tcgtcaatcg cctcatcgac cactgcgccc gcctttgatc
    18121 cggggcagag tgctccacat ccgggtcgaa cacgggaaat cgcttgccct tttccctctt
    18181 cctgcgtact ttgttgttgc gcttttccga atcaattcat gaggtattcg attatgaagg
    18241 ggttgaaacc cgccctgttg ctgcttttgc cacttctcct gacctggtgg tccgccggtc
    18301 gcgcccagat tacggaggcc gagatcacca gccaggaact cctgaaccat atcagctttc
    18361 tcgccagtga cacgctcaag ggacgacgct cgggcagccc cgaggcggag agggcggctg
    18421 aatatatcag agaccagttc gccgctgacg gcctcgtcct cctgggcaag gaggggctgc
    18481 agagtttcga ggtcctcaac agtctctccg ccggtgaaca caaccggctc agcatcgggg
    18541 gagagcatgc cgtcccgggc agggatttca cccccctcgc tttctctgaa aacggggagc
    18601 tcgccgccgc cgtccacttc gccggctacg gctttgccat cgagaccgac tccctcacct
    18661 ggcaggattt tgccggacag gagctgaagg ggaaatgggc cctcatcctt cgcgacggcc
    18721 cgccccctcc cgcatcgggc caggaccgct ttgagcccca tcgcagcctt cgcaagaagg
    18781 cccaggctgc gcgggaccac ggcgcggctg gcgtcctctt cgtcccggca gctgcgcagg
    18841 aagaggagtc actgccccgg cttaacgccg accagggcct cggcagcctc ggcatccccc
    18901 tttttcatat ccgccgcagc ctcgccgacc gcatcctcgc tggcagcggc gttacccttg
    18961 ccggcctgca gcagcagctc gattccaccc tgacgcccgc cagcatggcc ctcccctgca
    19021 cagtcgacgc ccgcgcggag gtggtgcgcc agatgggccg cacccataac gtcgtcggcc
    19081 tgctgccggg gagcgatccc ctcctcaggg atgagtatgt gatcatcggc gcccattatg
    19141 accaccttgg catgggcggc cccggatcgg gctcccgccg cccggatacc ctcgccgtcc
    19201 ataacggcgc cgacgacaac gcctcaggcg tggccggcct cctcgaactc gcggaaaaat
    19261 gggccgccgc cacagaaaag cccaaacgct ccctgctctt cgttgccttc gccgccgagg
    19321 agatgggact gctcggctcc aaacattttg tcgaccatcc cctcgtcgac ctcagccgcg
    19381 cccagctgat gattaatatg gacatgatcg ggcgcctgaa tcccgcaacc cgcgccatga
    19441 ccttcggtgg caccggcacc gccgccggac tggacacgct ggtcatctcc ctcgccgccg
    19501 cccatgatct ccatccgacc ctttcgcccg agggctacgg cccctccgac cacgcctcct
    19561 tctatgccag ggatatagcc gtcctctttg tctggaccaa catctccgaa gagtatcaca
    19621 cccctgagga tgatgcctgg aggatcaatg cacagggaga gcaagatgtg gtgagcttgg
    19681 tcttcgacat cagccgcgaa gcggccgacc gcagcagccg gctcgccttt caggaggccg
    19741 gccccaaatc ccagccggcg acctctcgcc gcttcaaggt caccctcggg atcatgcccg
    19801 atttcacctc caccagcggc aagggattgc gggcggatgc ggtcatgccc gaccggccgg
    19861 cggccagagc cggcatgaaa aagggcgaca tcatcatcgc catggaaggc aagccggtcg
    19921 gtgatatcta cgagtacatg aaccgccttg ccgattttcg cgtcggtcaa cgcatcagca
    19981 tggagatcct tcgcgatggc gtcaagcaga ttcttatcgt ggagctctga cccgaccact
    20041 ccggagccgc ctcaaagggc agtatcagat cgcattcggg gtgggagggc ttgatgagag
    20101 ctttcattct ggtcggaggg ctggggaccc gactgcgcgc cctggggatc acacagccca
    20161 aggcgatggt cgacattgga ggccgcccct ttctcgaata cctcgtgcgc tacctcgccc
    20221 gccacaatct ccgcgagatc gtcttttgca tcggctacgg ggccgaggaa atccgctcct
    20281 ggtttgacga cggcagccgc tggggtgtaa agattctcta ctcggctgaa aaatctcccc
    20341 tcggaaccgg cggcgccctc aagcaggctg aatccttcgc cgtggaagaa aatctggtct
    20401 gcaacgggga ttcctttctt gaactggagc tgccggactt tgccaacttt catcgcaaac
    20461 gaagagccct tgcctcaatt gctgcgataa acgtccattc catgggtgat tacggaaaca
    20521 ttcagaccgg tcgtggcggg cggatcaatg ccttcgccga aaaaagtgca gggggaaggg
    20581 ggctcatcaa cggcggggtc tatctgctgc agcgggaggt ttgggagacg atcccaggcg
    20641 gcgggccgct atccctggaa agggagatct ttccccgcct ggccgaaggc ggccgctgct
    20701 acgccttcaa gaccgcgggc tactttctgg acatcggcac acccgagcgt ctcgaccgcg
    20761 cccgggccga actgccgtcc gtgatgcagc gcattctccc gtaacagccg gtccgcacac
    20821 cgggcggcca ttcaccgcac acctgatcaa gagctgatat gatcatacga gccaaagcgc
    20881 cattgcgcat cagtttctgc ggcgggggca ccgatgtcgc cccctatccc gaggagaggg
    20941 gcggcgtcgt gctgagcgcc accatcgaca aatacgccta cgcctctttg atcacgaagg
    21001 aggaggcgcg gattcgcatc acctccctgg attatgacac cgaggccagc tatcggctga
    21061 acgagcccat ccctttcaat ggcgaactcg acctggccaa ggcggtcgtc aagcactttg
    21121 cgccccggac cggcctccat cttttcctgc attccgacgc ccctccgggc tccggcctgg
    21181 gctcctcctc gaccatgaca gtcgccatgg tgggggtctt caagcagtgg caaaaccatc
    21241 ccctgaccga ttacgaaatc gccgaattga ccttccggat cgaacgcgtc gatcttcaga
    21301 tcgtcggggg caagcaggac cagtatgcgg ccaccttcgg cggctttaat tttatcgaat
    21361 tctttgacaa gacggtggtg gtcaatcccc tgcgcatcaa cgacgcggtc atcaacgagc
    21421 tggagtacaa cctgctgctc tgctataccg gcaaaacccg cctttccgcc aacatcgtcg
    21481 ccaatcagac ccgctcctat caggagcgca aaccggccgt ggttgaggcc ctggatcaga
    21541 tgaaggccct taccgtcgag atgaaaaact gcctgctgcg gggccggctg gatgatttcg
    21601 gccagcttct ccacgccgcc tggctgcata aaaagaacct cgacgccaat atttcgacca
    21661 gccagatcga ccagctctat gaaaccggca gggaaaatgg cgccctgggc ggcaagatcc
    21721 tcggcgccgg cggaggaggc tatctcctct tctactgccc cttccagaaa aagcatcggg
    21781 tcgccgaagc cctggaaaaa gcgggcggac aggtggtgaa attcagtttc gaacagcgcg
    21841 gactgcaaat ctgggatatc cgatgaaaat cgttctggtc ggcacctctt tccccatgcg
    21901 cggcggcatt gcccattacg tcgcccttct ctataaaaaa ctgctcgaca tgggccacga
    21961 ggtcgaaatc atctccttca agagacagta tccggatctg cttttcccgg gcaagaccca
    22021 gcaggacaac agccagcaga ccatcgaact gccctcacgc cccctcatcg actcgatcaa
    22081 tcccctctcc tggatcagaa cctttctcta catccgcagg accaagccgg aactggtggt
    22141 ctacaagtac tggatgccct tcttcgcccc ctgctacggc acgatcgtcc tcctcacccg
    22201 tctcttcacc cggaccaaat cgctctatat cggcgacaat atcgtgcccc acgaacagat
    22261 cccggtcgtg gactggctcc tgaccaaatg ggctctctgg aaggtggact atttcatcgt
    22321 ccaatcccaa accgtcgaga acgacctgct caaaatcaaa ccccacgccc gcttccggca
    22381 ggtgccccac ccggtatatg aaatcttcag ctccagttac acccgggaaa ccgcccgcgc
    22441 ccggctcacc cttggcgaag aagagaaggt cctccttttt tttggctatg tacgggctta
    22501 caagggcctg cactacctga ttgccgctct gcccgagctt ctgcgtgaaa tggaggtacg
    22561 gctccttgtc gccggcgagt tctatgagga tgaggagaaa taccgcctgc aaatcaggga
    22621 gctgggactg gagcaggcga ttctcctcaa aaccgattat atcccgaatg aggaggtggg
    22681 cctctacttc accgccgccg atgtcgtcgt gctgccctat gtctcagcca cccagagcgg
    22741 catcatccag atcgcctaca actttgacaa acctgtcatc accaccgacg tcgggggcct
    22801 tccggaggtg atcgaaccgg gcaggaccgg ctttgtcgtc ccggcggagg acagccatgc
    22861 cctggcccag gcgatcctcc acttttaccg tgtccgggaa gaggccgatt tcccggaatt
    22921 catcaaaacc tacaaaaaga gattttcgtg ggagaatctg gcgaacgcca tcaccggctt
    22981 tgtctgagtc tggcacgata atcgcgcatg accttcaatt aatcaaaagg caggcgagat
    23041 gatagtcgaa ctggagcaac tcaccttccg ccatctgctg cagcgcagtt tccgtgagta
    23101 cggagctctg cccgctgtcg cgagcgtgga cgaagagccc atcacctacc atcagctcaa
    23161 ggccaggatc gaggagacca tggccctgct catgagctgt gaggtcaagc cgggtgaccg
    23221 ggtcgtcatc ctcggcgata acagtccgaa ctgggtggtg gcctacctgg ccatcaccag
    23281 catgggggcg gtcgcagtgc ccatcctcac gggttttccg gaggaggata cccgccacat
    23341 catccgccac tcggaggcgg tggcggtttt cgtctccggc aaaatgcgca gcaaaatcga
    23401 agaactggag cactcggaac tccgcaccct catttcactc gaggattgga gcatcgagga
    23461 ggccgttcaa gagcgcacct tccttctggg caaggagacc ctcttcaggc cggcggaact
    23521 caaatccctc cctcttcccg cagcgatcgc cgcggtcgag gcggatccgc cgcaggagga
    23581 tgatctggcg gtgatcatct acacctccgg gacgacaggc cattccaagg gggttatgct
    23641 cacccagcgc aacatcatct atgatgtcgt gcacgccctc gagcgttttc ccctctcgcc
    23701 gtcggaccgc ttcctctcga ttctgccgct cgcccattcc tacgaagcca caggcggtct
    23761 gctcgccccg ctcgccaccg gtgtgtccat ctattatctc aagggactgc ccaccccggc
    23821 caagctgctc gccgccatgt cctctgtcca cccaaccggg gtcctgactg tgcccctggt
    23881 ggtggacaag atctatcgca aaaagattgt gccgcagata tagagcaaaa gggtgctcaa
    23941 cacgctctac cgccggcccg tttttcgtaa acggctgcac cgcatggcgg gcaaaaaact
    24001 catccactcc ttcggtggaa agctgcgctt cttcatgttc ggtggggccg cgctcaatcc
    24061 ggacaccgaa accttcctca tcgatgcggg catcagctac tcctccggct acggtatgac
    24121 cgaaacctct ccgatcatga ccattaaccc cttcggatca gtgaagatgg gctcctgtgg
    24181 caaggccatc ccgggaatcg ccatggccat tcagcatccc gacccgcaga ctggaatcgg
    24241 cgagattatc gtccagggcg atatcgtcat gcagggctat ctgaaaaatc ccgccgccac
    24301 acgtgaggtg ctgctcgatt cgggctggct cagaaccggc gatctgggtt tcttcgatga
    24361 agagggttat ctattcatca agggacgttc caagaatgtt atcgtcggcc cctccgggga
    24421 aaacatctac cccgaagtgg ttgagcagga actctcgcag tccccctata ttcaggaagc
    24481 cctggtgcag atggagaaga aaaggctggt ggccagggtc tatctcgact acgactttat
    24541 cgaccagaaa ttctccctca acagcctgga gccccaggag gcagagctcc ggttggccgc
    24601 aatcctcgag caggtgcgcg ccgagaccaa tggccggctg ccgtcattca gccgcctgca
    24661 gaccctgatc attcatcctg aacccttcga aaaaactccg accaacaaga tcaagcgcca
    24721 cctctattac agcgccgacc aataaaaata gccccggcct ctgccggggc tttttcatgc
    24781 acggaggtaa cagcgaggtt aaaactcgtt aatcaatccg acatggattt ttccgccgag
    24841 cagatcgtca ccacggccga ggccgtaatc gaggctcatg atgcccagcc ccgtctccag
    24901 acggaagccc aaaccgtagc cgagtttgaa ctcctgaagc ttacctgatg ccctttgtgc
    24961 gctgtaatag ccatagtcgg cgaaaaggaa ggtgcgcgaa cgccgtccca gccagtagcg
    25021 gtactcgcag ttcagccagg ccaccgagct gccgcgaaac tggtcttcgc tgaatccgcg
    25081 caaagtcctc gcccctccca ggcgaaactg gtccgacacc ggcacaatcg tctcaccact
    25141 gacgaattgc cggccgtgaa gcccaagggc tacgatctgg cgttgaaaaa gcggggtata
    25201 aaactcgaga tcgagtgtta tcctccgggt gccggtcttg cgtttgacct cgccggtcag
    25261 caggggctgc ggcccaaggt tctccttgcg gccgctctgg tacgtagtgg cataatagac
    25321 cccctggcgc ggattgagga ggtcatcccg gctgtcgtat tcgatgccga tggccgcatt
    25381 cagagtgcgg ctgcgcagca ggccgaattt gtaactgccg attgaatcgg gaagtatctc
    25441 cgtgcgtccg gcctgggcct gaatggagag ttgatcgaac agggggatct ccacttccag
    25501 actgagatcc cgctggacat aagtggtgtc ctgtatcaac tgggaaaaac cgccgccgat
    25561 atgcaggggc cagcccgcca gccacggttc ccgatagcgg agactgatat cctgggtgcg
    25621 acgatccttt ttctgccaat gcgcggagag agcgcggcct gttccgagca gatttcccag
    25681 tgaaatgtcc accagcccgg tgaaatagcc cttctcatca ccacttgcgg gagaatagcc
    25741 caccactcca tcaaaacgac tgctttggcc ctcctcaacc cgcagcagca gcccgccttc
    25801 atcccccgag gtatagaaga ggatgggctc ctccaccttt ttaaaatagc caaggcgcat
    25861 cagccgcccg acgatccgct ccaccttttg ctgagaatag cgctcgccgg gcttgatacg
    25921 gatctcgcgc aatatcactc cgggcctggt caaggtgttg ccggcgagct ggatctcgcg
    25981 caggatcagc cgcggcccgg gcgcagctga atagcgcagc tgcaggccat cgcgtgcgga
    26041 atcgaggctg tcgaggcgga gctcctgcag ctcaagcctg gcgaagggat gtccctctcg
    26101 atccatttgc agcagggcat catccagatc ctcttccagc tccgccccgt caagcctgcc
    26161 gcccggacgg gtggtgaaac gctgtacgag tctccgggtg gagatgctgt ccaatccggc
    26221 gagctccact cctcccgtgc gcagctctcg tccctcatcg agatagagcc gcagctccgc
    26281 gccgctgctg tccgccgcga cgcgatagac gatcgaatcc aggcgggcat accagtagcc
    26341 cgcagccgcc agactctcca gcagcctgcg gccgcgcagc tgcagatccg ccgcgacgaa
    26401 gggatccccg cgcttgaggc cgaactccgc acgtacctcc tcttccccca gggtgcgcgc
    26461 cccatggatc gtgatcccgg tgaccgggat ggtgcgcggc tgggcgaagg atttgggaag
    26521 ggcggccagg ccgagcagaa gaaatgtcag ggctatcctc ttcatcattc ccttccgcct
    26581 agcgaaatgc agccgtgatc tgggcacggc cggtatgggt tacgccctgc cgttcgggct
    26641 cattgcgtcc cgaataggaa aacgaggcct gcacggtctg ggagaggcga tattcgaagc
    26701 gggcatccca acgcatcgaa tacccggcgc tgcggccgtc cgccatttca taggggatga
    26761 tgcgcccttc gggtgcaacc gccacccggt tccattccac ttcggcctgc cagcggccgt
    26821 tgctgcgcca tgaataggtg acacgcggcc gcagggaaag atagttgacc cgggttgcgg
    26881 gtgcgaaaca gcggtcctcc tccatggcga ggctgccctc cagcgcgaat tccagcgccg
    26941 gcttgggacg ataggaaaac tcgactcggc cctgatcgga gaggatgtcg cggtcctgcc
    27001 ggctgctctc gctgaagctg cgcgcaatgc gtttgcgggc gaaatcggac tggctgatca
    27061 accgtgcccc gatgcggccg gtcaggcgtg cactgacctc atcatcgctg cgctcctgtc
    27121 cgccctccag gtactggttg ttcatctcct gcttttgctg atagcgcagc cgcagagaaa
    27181 aatcccgccg tttttcgaaa agaaaaatat cctgccgcca ctgcatcgag ccaaaaagtg
    27241 taaccgcagg ctgctggtat ttgtccagat tgagcagata gatctgccag acctccttct
    27301 cctgggtccg ctcctcaatg gccgaaaagg tttcggtcga gaccgcgctc agccatttct
    27361 gccaggtgcg caggggtttt ccaccttttg tccggttcca gaagcgggag ggatccaggc
    27421 gcagccgccc gctcgtcttc agttcgacga ccggcaccag ctcatctgtg gtgaggatgc
    27481 ggaggatgta atctccgagg ggatcgagga catattcgcg cagattctcg tcaaaacggt
    27541 aggaaccatc gcccgaattg accttgatat agaggcgttc ctttttggcg gtggcggtgt
    27601 tggaggtctg ataattaaaa tcggcgttca gggcgctctt ccagggagtg agtagcagct
    27661 tgagttcagc caggtcggtc tcctttctgc ccaccgccga gtcggcgtag gtgcgcgcgc
    27721 ggtgggtgaa ctcgagagat cctgagaggg cgcccagctg ctgcgcctgc agccttaact
    27781 gctgggtgcg ggctgtcgac ttgccggcga actcaccgcc cccggagtag gattgatcgt
    27841 cccgccagga gtagagcgcc cgggccgtca cccttttaaa cggatgcaga tcgaggccgc
    27901 cgctcatgga atggaagcgg aatccggtcg ccagggaatc agaccagttt tcttttttct
    27961 cctctccctc gtattcgagg aggggactga cggctccaat tttgtaatgc gcctgaccgc
    28021 gcgcacgcag ccagtcgccc ttttctctcg cgggactgtt gttcctggag atgcgttcga
    28081 tgcgcatgga ataatcgggc cagcgattcc gggtcagacc atgttcgaac tgccagcggc
    28141 tggagcggaa atccccgctc ttgcggatat cgccgtactc gccggccagc tgcgcgccgg
    28201 caaagggggc ccagcgcccg gccatctccc agacccgctc cccccgtgcg gcgctgtcgg
    28261 gcagatccca gcgccggttg tactccgcct cgctggtgcg gtcgatatcc cgaaaacgat
    28321 tctgcacctg acggaaccgc cccgaaagct ccatcaagcc gaggctgcgc cggccccatc
    28381 gcaaggtatc gggggtaacg agcagctgcc agttctgcgc catcccctgg ttgtcatcgt
    28441 cgcccagccc ggaccaggta ttttgatccc tgctgctcat ggccatctct ccgctcaggc
    28501 gcagggcccg atacggctcg gcagcgatct gcacatcgaa aagggtgtga ctctcggcag
    28561 tggtcagaag ctgaaccggc ttgtaacgtc cggcccctgg gccgatatat tcataggcac
    28621 cggcgcctgt gtatttgtat tccccccggc cggcgccgac atccgaaaaa gtgacgatat
    28681 aatcgccgga atcggctccc acgtaggtat agatcccctc cgcgctccga acatagcgtc
    28741 cccggccggg gccgacatag ctgcctccct ccacagcagc gagggccgaa ttgtcgcccg
    28801 ccgcgcgcag aattttgagt ttgtcttcat ccagagtgaa atcgagggga ttgtccttgt
    28861 catcgctttc gtggatcagg gcgccggtga gctgcagccg cccgctccag aacgagccgg
    28921 cagtccgggc cgcatagatg ctgcgccggt atttctcatc tgagaactgg aaatcggcga
    28981 cgatgcgcga atcagcggtg atgagccgcc gacgggtgaa ggtgatctgc gcagaggcgt
    29041 aatcgatgat gtaatcgttg gtttcgccgc gcaccatctg ctggccgtcg aggtagatcc
    29101 gctcggtgcc cgccagtacg atcatgtcga tctggccccg gtctcccttg agctgatagg
    29161 ggccttgatt accttcctgg ccggtgatgg attggctgat gtatttgccc cgcgagaccg
    29221 cgccggaaaa agtgccctgc agattctgaa agtgcgcata acccattgct ccttgcagtt
    29281 tccggctgta ttgagcgaac tgggagccgg taaaatcaag ggtataatca cccatggttg
    29341 cggagagatg gggggaattg atctgcacaa agaccttgtc gatttcctgc aggttctggg
    29401 tggtgccctc gggctgaatg gggg
//
LOCUS       DAOPWU010000007        29254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_1537_length_29254_cov_5.568615, whole genome shotgun sequence.
ACCESSION   DAOPWU010000007 DAOPWU010000000
VERSION     DAOPWU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 29254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29254
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_29254_cov_5.568615"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKM23_01025"
     CDS             complement(<1..329)
                     /locus_tag="PKM23_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNY90080.1"
                     /translation="MIRKLSFAELEAERLKRTSCVRSGPPILTALLDDIRSLHNVGAI
                     FRTADGAQFDHLYLCGITGTPPRNEIRKTSLGAEESVPWSYHADPVELAGRLKAEGYQ
                     IVVLEQT"
     gene            complement(436..738)
                     /locus_tag="PKM23_01030"
     CDS             complement(436..738)
                     /locus_tag="PKM23_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90081.1"
                     /translation="MNKKLILFVALLFVFAIAAGTFHAVNAADDKAKSSTVQKDDCCK
                     KNAADCKEKAADCKEKADAKECAKEKSGCCSKAKTEAAASEAKCAGCPAKSACEKK"
     gene            complement(785..1300)
                     /locus_tag="PKM23_01035"
     CDS             complement(785..1300)
                     /locus_tag="PKM23_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90082.1"
                     /translation="MAKRILLCLIALALIGGCGKKEEKERKQTSLPSVGDNADNGGML
                     RFTLEGKAMRDRFVVAQFTPRGDLFMEDNLQIYNYDINSDKYPRLLMSVNTRESDLKK
                     WQGQTLKAEVVAFTVSALSAPLTSQGELRITRVNDQFVEGQFHGELLSEENGKSFEIR
                     GEFKAVLRWNV"
     gene            1492..2742
                     /gene="pepT"
                     /locus_tag="PKM23_01040"
     CDS             1492..2742
                     /gene="pepT"
                     /locus_tag="PKM23_01040"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNY90083.1"
                     /translation="MIAIDQSCVERFLRYVSFDTQSSEESASYPSTEKQKQLGRQLVE
                     ELREMGLANAGMDEWGYVTATLPANVSHPVPVIGLIAHMDTSPEVSGAEVKPIIHKNY
                     QGGDLVLPAGVVIRAEETPELVRQIGNDLITSDGSTLLGADNKAGIAEIFSAIRYLID
                     HPEIPHGRLRIAVTPDEEVGRGTEHFDVQAFGADFAYTIDGETLGEVEDETFCADSVI
                     MTISGVNLHPGYAKGKLVNAIKIAARIIANLPQDALSPETTSGREGYLHPHHLTGTVE
                     EAVIKFLIRDFTIEGLKEKESMLQQWADKTMKTYPKAKLDLRVEESYRNMKYEINKEP
                     RVVEHAVEAVRRAGITPIQNIIRGGTDGSRLSYQGLLTPNIFTGGHNFHSRQEWISIQ
                     DMQKAVEVIIHLARIWAEEAAGVA"
     gene            complement(2857..3321)
                     /locus_tag="PKM23_01045"
     CDS             complement(2857..3321)
                     /locus_tag="PKM23_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNY90084.1"
                     /translation="MRLLEYINKNAIFLDIEVHDKNEAISEIVESMAKAHLIQNAEEF
                     KKEVYERERLGSTGIGKGIAIPHARTRAVNRLVIAFARLKEGVDFGAEDDDPVRLIFL
                     LGTPVDTVGDYLKILAKLSRLLKEDLLRKRLLKAKTGKEVLELLEEAEGQNC"
     gene            complement(3491..4909)
                     /gene="pyk"
                     /locus_tag="PKM23_01050"
     CDS             complement(3491..4909)
                     /gene="pyk"
                     /locus_tag="PKM23_01050"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNY90085.1"
                     /translation="MRKTKIIATLGPASREPAVLERMIDAGMNVARINFSHSTHQEHA
                     ALIAQVREAAERKGRPVAILQDLQGPKIRTGLLAGGKAVELRAGRPFMITTDECPGDA
                     ERVSTSYAALPDDLRPGDRVLLSDGLMELRVVQVSGREVATEVVTGGLLRAHQGINLP
                     GVALSAPALTEKDREDLAFGLAQDVDLMTLSFVRRAEDIREIKALIAAAGRNIPVVAK
                     IERAEALTELPEILAEVDIIMVARGDLGVELPPEQVPVLQKQLIEAANEAGRPVITAT
                     QMLESMTVNPRPTRAEASDVANAIIDGSDAVMLSGESAVGRYPVEAVAMMARIAEAAE
                     ASERHGDAAATRRWLIERPQSGPLAIAAAAATIVKQLPIAAICVFTLSGETARLISQQ
                     RPQAPIFAFTADRDAWRRMSLMWGVVPQLITFSSDMETLWQRVQPSVRRAVPQEGAQV
                     ILVGGHPFGEGSRTNLLKIQSL"
     gene            complement(4906..5538)
                     /locus_tag="PKM23_01055"
     CDS             complement(4906..5538)
                     /locus_tag="PKM23_01055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNY90086.1"
                     /translation="MESGTELIALRHGETEFNADRRMQGHLDVPLSEVGREQARLAAA
                     RLAAEPIDKIYSSDLQRAVETAQTIRGSRGIDLVTDVRLREFHMGTFQGLTVTEARER
                     HGDAWERFFIHDADFALPGGQSRNQKQVEIAAFMEEVVRTEAGRRLLVVTHGGILIGM
                     LRHVLGIPASHYFRVSIENTGIQRFFYRNAAWHLISWGEVDHLTVARESR"
     gene            5733..6740
                     /locus_tag="PKM23_01060"
     CDS             5733..6740
                     /locus_tag="PKM23_01060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNY90087.1"
                     /translation="MRRFIIAALFTALVATGCGGPKGAKLEKGTPIYDLAVELAKAYP
                     AVDPDANKTLVKCKYFQITPGTFFTEMQLAMGKNIDQIKTLDPKRLPDFLKVNIERMG
                     ERNLLLEGAKKAGVAITPAQVDSTLQFIYTQNGGEEAFIQRIGSDGISIETVKADIER
                     EMVIRKYFDKLYNEVKVTDEDLNAAYNQDKTASVRHILLMTQGKDEAGKADVRKKMEE
                     LLRRAKAGEDFATLATENTEDPGSKTSGGLYENFPKGHMVKPFEDAAFSVPVGEISDI
                     IETQYGYHILKVVDRKKETRPLAEVKSELEQQLLRGKRRDLNSSIVEKLKKDSGYQIF
                     V"
     gene            6843..7349
                     /locus_tag="PKM23_01065"
     CDS             6843..7349
                     /locus_tag="PKM23_01065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011647609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY90088.1"
                     /translation="MPSQKPDYTIRPATAADVPTLLELIHGIAEYEKLSHAVANSEEA
                     LLAAGFGPDPYFRALLCEKGTEKRAVGFALYFYTYSTFTGKPSLFLEDLFVRPDERGS
                     GFGKALFMELVKIARERGCGRMEWSVLDWNRPAIDFYLRLGAKAMDEWTVYRLDEAAL
                     QNLAGRQG"
     gene            complement(7346..8563)
                     /locus_tag="PKM23_01070"
     CDS             complement(7346..8563)
                     /locus_tag="PKM23_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNY90089.1"
                     /translation="MPRNETGLQIGIVGAGPAGMMAALQAVRTGGRVVLFDANRTVGR
                     KLAVTGNGRCNITNSRIDPDRYAGAEAGFVQQVLAGFGHAELVAFLEGIAVPLHVMAD
                     GWCYPRSGSAAAVVAAFAAALEQAAIELHLGETVRSFFPAMSGWGLSAQEEYRFDRLI
                     VASGGLAQPKLGARGELFAQLERLGHTVIAARPGLAPIVADMRRLHKMQGVRMDVGLQ
                     LWRGKSLLGQSEGNAIFTQWGLNGPAAMDLSHLVSGEKGELELAVNFIAGAEPLMQEL
                     LQRFADSEWPVGIVLSGLLPPKAAHFVVQSCGVAAEVGMRRLGEREKAALWSALCNTR
                     LQVRGTRGFEFAQVSAGGIPADEVVSRTMASRKQAGLLLAGEVLDVIGPCGGYNLQFA
                     FSTGALAGIAAAS"
     gene            8946..9146
                     /locus_tag="PKM23_01075"
     CDS             8946..9146
                     /locus_tag="PKM23_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNY90090.1"
                     /translation="MERGTVKWFNTSKGYGFITRESGGDIFVHFSGIVGDGFRSLNGG
                     DAVEFEVEETQKGPQAVKVTKV"
     gene            complement(9198..10229)
                     /locus_tag="PKM23_01080"
     CDS             complement(9198..10229)
                     /locus_tag="PKM23_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNY90091.1"
                     /translation="MSLTWIVVILTVAMALIFDAINGFHDSANSIATVVSTRVLSPRN
                     AVIWAAFFNFIAMFIFVPRVADTVSKIVRIQAGDPAFVYVVLAGLVGAIVWDVLTWWL
                     GLPTSSSHALIGGFAGAGIAFQGTGVIRWEKIWATVRFIPLSPLIGMALGFVIMIAVY
                     WLFRRWRPYDVDKLFRKGQLVSAALYSLGHGGNDAQKTMGIIVALLVAAGALDENTQL
                     SLLDPRTAWIILSCQIAMALGTALGGWRIVKTMGMKLTKLKPVHGFCAETSGAFTIFF
                     ATHWGIPVSTTHTIAGAIVGVGSTTHASSIKWGVAMRIIISWLVTIPFSALTAALVFW
                     LIKLIHPVF"
     gene            complement(10266..10889)
                     /locus_tag="PKM23_01085"
     CDS             complement(10266..10889)
                     /locus_tag="PKM23_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNY90092.1"
                     /translation="MFKSLLPKEYAFFDSFADHARIVQTASRELLRLMRGEVDTQVGI
                     QAIKDWEHEADDITHRCIDALNRTFITPIDRPDILRLIKTMDDVLDCIDAGVIRMGLY
                     DIRIMREESVAMAEILLACSDEIALAVQELRDLKNQESINKRCMTIHQLEHQGDEVLR
                     AALQRLFREEDAVLIIKWKDIFERLEKSTDRADTVAVIIEGIVISAS"
     gene            complement(10948..11658)
                     /locus_tag="PKM23_01090"
     CDS             complement(10948..11658)
                     /locus_tag="PKM23_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNY90093.1"
                     /translation="MLEKISSIFIWIFGLAIAVPALLMIILMAVTLPVRIYNRPGRAL
                     LRLIVRVFGGRATVEGVDKLDPHKTYLFMPNHVSLFDIPLVGGYIPNYARGVEAAEHF
                     SWPLIGWFVRSIGNIPIERGSIHSSWSSLEKAASQIRGGKSIIIMPEGTRTCTGRLGA
                     FKKLPFRLAQIAGVDIVPIGLSGLFRFKPRMGWIVKPGPLKMKVGDPIPYENFKGLEL
                     QELSDLVHERIESLIEYP"
     gene            complement(11944..12714)
                     /locus_tag="PKM23_01095"
     CDS             complement(11944..12714)
                     /locus_tag="PKM23_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNY90094.1"
                     /translation="MSDLAYSIVTRDLSLWYGEFQALRSVSFKARNGLITALIGPSGC
                     GKTTLLRCFNRVNERYGNVTTTGEIRLLGKNIYDEDVSLIELRKSVGMVFQRPNPLPL
                     TVYENIVFGLHIHSPARLNASALDEAVEQALTDVGLWMDLKDKQKSKATSLSLDQQQK
                     LCIARLLPLKPEIILMDEPCSALDVEATRQVEELMLELAGRYTIIIVTHNMAQAKRVS
                     HECAFMLMGELIEHSRTEDLFIRPLDKRTAEYIEGRFG"
     gene            complement(12711..13475)
                     /locus_tag="PKM23_01100"
     CDS             complement(12711..13475)
                     /locus_tag="PKM23_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNY90095.1"
                     /translation="MVEQTPKIGVRELRLSYGQHEVLHGISFEILRNEIFGLIGPAQS
                     GKTSLLRAINRTLEFTAGARYSGSITVDGEEVGKVRNVYELRRKIGMVAPLPVGLPLT
                     IYDNVAFAPRMGGIRQKGSIDDIVERCLRQAALWDEVKDRLHTLGTRLSGGQQQRLTI
                     ARALSHQPEILCLDEFSIAIDPVTTMRIEEVLKELRQQMTIILVTNLVQQARRLADRT
                     MFLFEGDIIELDRSEVIFSNSPAQQKTYDYVNGIFG"
     gene            complement(13469..14353)
                     /gene="pstA"
                     /locus_tag="PKM23_01105"
     CDS             complement(13469..14353)
                     /gene="pstA"
                     /locus_tag="PKM23_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNY90096.1"
                     /translation="MFEATDQTRRKQRQESAIKYLLTTMTVIIVLPLLLIIFYLFYKA
                     APILSLEFLLENPIHGMRSGGIWAPLLGTIYLVLISLLIATPVGIFAAIYLNEYSRES
                     WMTRVINLAVVNLAGVPSIVHALFGLGAFVLFAGIGRSILAASLTLAIMTLPVIIAST
                     KEALAAVPHSFREACWNVGATRWQTIRNVVLPNSISGILTGVILQVSRAAGETAPVMF
                     TGAVFFKAIQTGDPFAYGLLDQCMALSMHLFTISTQVPDVPDALTYGTAVVLLGVVLL
                     FNALAILLRIKLRGKKKW"
     gene            complement(14369..15265)
                     /gene="pstC"
                     /locus_tag="PKM23_01110"
     CDS             complement(14369..15265)
                     /gene="pstC"
                     /locus_tag="PKM23_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNY90097.1"
                     /translation="MTPGRRHLEPLKEWLIERFIWLCGVSAIFFVFAIFIFVFREGAD
                     YFFSGFKAGEFFTSIEWYPTSRTHVRYGALALITGTFSVSFLAMFLAVPFGLGAAVFI
                     SEFCPPRLKEGLKIVIELLAAIPSVVWGFIGLTIMNSLIVRLFNAPIGLNMFNGAIIL
                     ALMSVPIIVSIGEDALKAVPDSYREAAVALGCTRWQMIFRVLLPAARNGLLAAVMLGI
                     GRAVGETMAVLMATGHSIRIPHGVLEPVRTLTATIAAELGEAPVHSDHYQVLFIIGIL
                     LFTITFVINITADFIVRGIGRK"
     gene            complement(15262..16197)
                     /locus_tag="PKM23_01115"
     CDS             complement(15262..16197)
                     /locus_tag="PKM23_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNY90098.1"
                     /translation="MKGPFSLLPLLLALLLAAGCLRGRQPGQRGTGQKQVIQNSGSDT
                     MVNLAQAWAEEYVRIKPAVSVEVSGGGSGTGIASLINGTADIANCSREMTFAERERAL
                     QNTGKDPVEHLVGYDALAIFVNLQNPLHEITLEDLAEIYGERGRIERWSDLGVQPPDG
                     KRDKIIIISRQSNSGTYEYFREAVLGKTRDFRLGTIDMHGSKDVVELVARTPGAIGYS
                     GMGYATDRVRMLRIARKHGETAYAPTVPNTQKGIYPVARPLFMYTLGEPEGELRDYLE
                     WIHSPEGQDLVVRSGYVSLSRTGSHAPAQGEEHQP"
     gene            complement(16218..17354)
                     /gene="phoU"
                     /locus_tag="PKM23_01120"
     CDS             complement(16218..17354)
                     /gene="phoU"
                     /locus_tag="PKM23_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNY90099.1"
                     /translation="MVTPHGESRYEQSLQRDIALLKRKTKEMAMLDEGGLRNAILALM
                     SRDRQLAYTVILRDRCVDDLEIELDRLCLEFLVRQQPVAIHLRFVFATIKIIRELERI
                     GDYAESIARQVLDLLPMQPIPPLDDFLELADVAISMFHDCVEAFLENDVEKARRLMVK
                     EDIADEMRNAINAKLIQWQEEGQLALEGMAPLMTVARRLERTTDQAKNICEEVIYMST
                     GQFIKHRHSDLFRILFVDADNSSLSQMAEAIGRAQSIGHFAFSSAGLEPAGEIKPAVR
                     RYLLERGFDLEGYEPKDFSVFLDTLPVDIIVALNTKVRKRIPAHFSNCVVFEWPISDP
                     AEEGGSAETIVERFDQAYTEISQHLRDLVQAIIGHDQYRSGLNQ"
     gene            complement(17468..18841)
                     /locus_tag="PKM23_01125"
     CDS             complement(17468..18841)
                     /locus_tag="PKM23_01125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNY90100.1"
                     /translation="MRRLLYLIILLIFFIALSAGMRSTTAPAYVEDEVLVRFKPATSA
                     KAVSVLTAAVEARVLSFSPSLTMHRLKLTGGMGVEEAVAHLRAQDQVELAEPNYRIKA
                     LATPNDPSFGKLWGLSNTGQTGGLADADIDAPEAWSTTTGSAAVLVAIMDTGIDYRHV
                     DLKANAYTNPGEDPWSDPNDPATGNKLDDDGNGLIDDWKGYDFHAKDNDPYDENGHGT
                     HVAGIAGAVGNNSLGVTGVCWQVRLVGIRVLDSSGDGNVGVAIDGIEYAVGLGARILN
                     LSWGGPDKSIFLEQAIDYAHNHNILVVAAAGNDGVNADVSPQYPACLALENIVSVAAT
                     DHGDQRALWGESDDNTDDCGFSCSNALAAVPGSNWGATTVDLAAPGKEIYSTVPGGYA
                     VFSGTSMAAPHVSGAAALLLARDPGLTDLQLKQRLLSSVDPLLSFNGLSVTGGRLNLQ
                     KALAGLP"
     gene            complement(18922..19635)
                     /locus_tag="PKM23_01130"
     CDS             complement(18922..19635)
                     /locus_tag="PKM23_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNY90101.1"
                     /translation="MDHRLELSRVEEIARGTIELTFHRPEGFAFLAGQNLNLKVPELL
                     FPDPKGPRRTFTIASAPHEPELRFATRLSGSGYKQTLQRLASGSLFEYLGPNGQFVYD
                     PEVESAAYLAGGIGITPFRSMLMHGVHAGFKAPTFLFYGNADPDSSAWHELFAGLAQA
                     EARFTYIPTMTNLAPEAPWPGERRWLSADLAAAYLPDPANTTYFLCGPPAMITALTDQ
                     LLEKGIKDNKIRYESLWGY"
     gene            complement(19653..21869)
                     /gene="rnr"
                     /locus_tag="PKM23_01135"
     CDS             complement(19653..21869)
                     /gene="rnr"
                     /locus_tag="PKM23_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNY90102.1"
                     /translation="MAKLSAGRDLGAEVTALFAREPQKSFKAKELARQLGIGKEEYLE
                     LRKTLRRLVQEGAILKLRSNRFGSASQSPVVTGILRVNSQGYGFVTRDDGREDIFVST
                     KQMGSALHRDRVRVRLFASRQGERPEGQVIEIVERGRDRIVGTLRWGRKYSYVVPDDI
                     KIQTDIIIPEPEGSGAEEGQKVVVVIDRWPSPHQNPEGHITHVLGFADDPGVDVLSII
                     HGFSLASEFPPEVEAEAARLGAAIPSGEHERRLDCRDWPIFTIDPADAKDFDDAVSLR
                     ALANGRLELGVHIADVSHYVAPGGAIDAEAEARGTSVYLVDRVIPMLPEHLSNRLCSL
                     VEGEEKLCYSVLMELSPAGALLGYTFRESIIRSRRRLTYEQAQAMIEGSGDDEISLLL
                     RKMWELAALLIRSREARGGIDFESQEVQVILDEQGHPTALKRRERLQSHRLIEEFMLM
                     ANETVARHVGVEMAAGLPEAPPFVYRIHEKPEREEVAGLLELLAQFGIVQELPKKITP
                     HYFQRLAAAIRRHPANFVLQDALLRTMMKARYSPENPGHFGLAYSHYTHFTSPIRRYP
                     DLMVHRLLKGYCANRVVEEMEALTGRLAEQCREASENEVRAAEAERASIKLKQIEYME
                     GHLGSEHDGFISRIVPFGIFITLPEFLVDGLVHISGLEDDYYIYEPSGYRLVGQRGGR
                     VYALGDKVRVRISRVDRNERLIDFVLVSKEHEERPKKMEAGAPFRRPQRGRKKSRG"
     gene            complement(21958..23337)
                     /locus_tag="PKM23_01140"
     CDS             complement(21958..23337)
                     /locus_tag="PKM23_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNY90103.1"
                     /translation="MNEGIFREYDIRGIVKTDLTNDVAYNLGRAYGTWMAKRGHKLVV
                     IGHDVRLTSPHLAKLFIDGIVTTGLNVYLVGEVMTPVLYYAILHYQTDGGVMITGSHN
                     PIEYNGFKMCEGLASLYGVAIQELKEIILRNGYVRTSSGRVTEKSIIPEYMEMMRAKF
                     AFPRKLKVVIDAGNGTAGPIAPELWSSLGIEVVPLYCDPDGHFPNHLPDPTVPKYMQD
                     LRQLVLAEKADLGIGYDGDADRVGIVDDRGRLVYADALMALLSREVLANHPGSTIIFD
                     VKCSQMLPEEISRLGGVPMMYKTGHSLLKARMKEIHAPFAGEMSGHMFFADEFFGFDD
                     GLYASGRLMRILAVSERPFSALVDELPLFVSTPEIRVDCPDQEKFAIIESMVKSFSAN
                     HETITIDGARVLFGDGWGLIRASNTQPVLVLRFEAKSRERLAEIIAIFRREMDKHPAI
                     AYNPDDLSI"
     gene            complement(23330..24196)
                     /gene="prmC"
                     /locus_tag="PKM23_01145"
     CDS             complement(23330..24196)
                     /gene="prmC"
                     /locus_tag="PKM23_01145"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNY90104.1"
                     /translation="MIQWSSDYLATRGFENSRLLSERLLAHVLELRRVDLYLQFDRPL
                     TPAELAHYKQLFLRLVAHEPLQYLLGETEFMSLPFAVGPGVLIPRPETELLVEKALER
                     ARAVAAREGMVRIIDLGTGTGCIAVSLAKNMPEASLVAVDLSAAALEWAERNSAAHGV
                     SSRVELVEHDFRNSPLSAWRGGFDLAVSNPPYIRSGDWAGLAAEIRDHEPREALLGGE
                     DGLDAYRSLAQMLPMLLKTGGEALIEFGDGMSAAVVAIFKQAGFAGAEFFRDLAGRER
                     LVHLFSPGGDHE"
     gene            complement(24247..25314)
                     /gene="prfA"
                     /locus_tag="PKM23_01150"
     CDS             complement(24247..25314)
                     /gene="prfA"
                     /locus_tag="PKM23_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNY90105.1"
                     /translation="MKEKLAAIAVRYFEVQRKLSDPEVLAKPHLLRDLAREESELSEI
                     VQPYQRLLKIERDIAGDEKVIEEGGDDELAEMARTEMEELSAERDELEEKLKWLLIPK
                     DPQDARDAIIEIRAGTGGDEAGLFAGDLFRMYQYYAERQGWTLEVMSINAQGIGGFRE
                     VIFALSGRDVYGKMKYEAGVHRVQRVPQTEASGRIHTSAASVAVLPEAEEIDVEINPN
                     DLRIDVFRSSGPGGQSVNTTDSAVRVTHLPTGLVVSCQDEKSQHKNKAKALKVLRARL
                     YDLALAEEQAKLTASRRSMVSTGDRSAKIRTYNFPQNRVTDHRIGLTLYQLDAVLQGD
                     IDEFVEQLQIADRSEMLKTMN"
     gene            complement(25326..26258)
                     /locus_tag="PKM23_01155"
     CDS             complement(25326..26258)
                     /locus_tag="PKM23_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNY90106.1"
                     /translation="MKEQESLAVGGQAIIEGVMMRGKDVVAMAVRKPDGGIVLKCTPF
                     KSIVKRFKVLNLPVFRGAVMLIESLFLGVKALNFSGDIAMMEENGGTGKAGRSENRWN
                     DVWMGMTILFSLALGIAFFFYLPLILTDLLGFESGWAFNLVDGVIRLVLFLAYIYLIS
                     LWKEIKRVFEYHGAEHKSIFAHENNQPLTPAGAMPFTTFHPRCGTSFLLIVMVVSVLV
                     FMFLGKPETVADRLIRIAFVPVIGGLSYELIKFSARAQRFALVRAFILPGLWLQRITT
                     NEPDEQQIEVAMVALRCALGQEVQAGPSPVEMVA"
     gene            complement(26355..26567)
                     /gene="rpmE"
                     /locus_tag="PKM23_01160"
     CDS             complement(26355..26567)
                     /gene="rpmE"
                     /locus_tag="PKM23_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNY90107.1"
                     /translation="MKKGIHPKYEVSTVSCACGATFKVRTTVGDEKLDICSSCHPFFT
                     GKQKLLDTAGRVEQFRRRYNLPNKQS"
     gene            complement(26657..27904)
                     /gene="rho"
                     /locus_tag="PKM23_01165"
     CDS             complement(26657..27904)
                     /gene="rho"
                     /locus_tag="PKM23_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNY90108.1"
                     /translation="MDIAELKARKIAELNKLAQDMGITGCTGLRKSELIFKILEEQTK
                     KEGLIFAEGVLEVLPDGYGFLRSPDFNYLPGPDDIYVSPSQIKRFGLRTGDTVSGQIR
                     PPKENERFFALLKVEAVNFENPEEAKSIILFDNLTPLYPHQRINLETTAGEISMRIMN
                     MLTPIGKGQRGLIVAQPKTGKTTLLQKIANSITTNHPEMKLIVLLIDERPEEVTDMER
                     SVKAEVISSTFDEPAERHVQVSDMVLEKAKRLVEYGKDVCILLDSITRLARAHNAVVP
                     HSGKILSGGVDANALHRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKG
                     TGNMELVLDRRISDRRVFPSIDVNRSGTRKEELLLGDQELNRVWILRKLLAELTPVEA
                     MEFLLSKMQGSKSNKQFLDSMSM"
     gene            complement(28117..29217)
                     /gene="ugpC"
                     /locus_tag="PKM23_01170"
     CDS             complement(28117..29217)
                     /gene="ugpC"
                     /locus_tag="PKM23_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNY90109.1"
                     /translation="MASVQLDKVSKIFDNQVRAVDAVDIDIRDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISAGEIRIDDRVVNQVPPKDRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKYPKDEIEQRVKEAADILSIEGLLDRKPKQLSGGQRQRVAVGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRTEISKLHTRLETTMIYVTHDQTEAMTMGDRIVVMKDGKVQQIDT
                     PMNLYDAPVNKFVAGFIGSPAMNFINGEIVRDEGLFFRTAGMKLRIPDSRSGLLAAHT
                     GRPVVLGIRPEDLSEYDLAPAQIPEMSALCRVEVVEPMGNEIFVYLANGGESLVARMN
                     VQSRPSVRADYRVQFDMNKAHFFMAESEEAIH"
BASE COUNT         6134 a         8961 c         8401 g         5758 t
ORIGIN      
        1 tcggtctgtt cgagcacgac aatctggtat ccctccgctt tcagccgccc ggccagttca
       61 accggatccg catgatagct ccagggcaca ctctcctcgg cgccaagact ggttttgcgg
      121 atctcgttgc ggggaggcgt gccggtgatg ccgcagagat agagatggtc gaactgcgcg
      181 ccatcggctg tccggaagat ggccccgacg ttgtgcaggc tgcggatgtc atccagaagc
      241 gccgtcagga tgggagggcc gctccggacg cagctggtcc gtttgaggcg ttcggcctcc
      301 agttcagcaa agctcagttt tctgatcatg gcttttacat accggtgtca gacagacatc
      361 aagtatttaa ccttgttcaa ggccataaaa aaagggaaag ccgcagggct ttccctttac
      421 acgcaaaggt gccggttact ttttttcgca agccgatttg gccgggcagc cagcgcattt
      481 agcctcgctg gcggcagcct cagttttggc cttggagcag cagccgctct tctccttggc
      541 gcattccttt gcgtcagcct tttccttaca gtctgccgcc ttttccttgc agtccgccgc
      601 attcttcttg cagcagtcgt ccttctggac ggtcgaggat ttagccttgt cgtccgccgc
      661 attgacggca tggaaagtgc cggctgcgat ggcgaaaacg aaaagcaggg ccacgaaaag
      721 gatcagtttc ttgttcatga cagcaactcc tttttaggtt atcggttcag ataagttaga
      781 cagattatac attccagcgc agtacagcct tgaattcgcc gcgaatttcg aagcttttgc
      841 cgttctcctc gctgagcagc tcgccatgga actgaccctc aacgaactgg tcattcacac
      901 gggtgatcct gagctcaccc tgggaggtga gcggggcgct cagggcggag acggtgaagg
      961 cgaccacctc tgccttcaag gtctggccct gccacttttt cagatcgctc tccctggtgt
     1021 tgacgctcat gagcaggcgg ggatacttgt cggaattgat atcgtaattg tagatctgca
     1081 gattgtcttc catgaacaga tccccgcgcg gggtgaactg ggcgacgacg aaacgatcgc
     1141 gcatcgcctt gccctccaga gtgaaacgca gcatccctcc gttgtccgcg ttgtcgccga
     1201 cagaaggaag agaagtttgc ttgcgttcct tctcctcctt tttcccgcag ccaccgatca
     1261 gagccagggc gatcaggcag agcaaaatcc tttttgccat ctcatttcct tttagtatat
     1321 ttaggcagcg cgatggcgcg gcgcagccgt cgaaggtgtt ttaatataaa tgattggaat
     1381 ggatatttca agcattgttt ttggcgcctc ctgtggccgc tgcatcttgc ccgcctgccc
     1441 cgcgctcctc aacccgaagc ttgttttata aaacatggga gtaatctgat catgatcgcc
     1501 atagaccaga gctgtgttga acgttttctg cgctacgtct ccttcgatac acaatcctca
     1561 gaagaatccg cctcctaccc cagcactgaa aagcagaagc agctcggccg ccaactggtc
     1621 gaggaactca gggagatggg cctcgctaat gccggcatgg atgaatgggg ctatgtcacc
     1681 gccacgctgc ccgccaatgt ttcccatcct gtgcctgtca tcggcctgat cgcccacatg
     1741 gatacctccc ctgaggtgag cggagcggag gtcaagccca tcatccataa aaattatcag
     1801 gggggtgacc tcgtcctccc cgccggtgtc gtcatccgcg cagaggaaac ccctgagctt
     1861 gtccgccaga tcggcaatga cctcatcacc tccgacggca gcaccctgct cggcgccgac
     1921 aacaaggccg gcatcgccga aattttcagc gccatccgct atctgatcga ccaccccgaa
     1981 attccccacg gccgcctgcg catcgctgtc acaccggacg aagaggtggg acgcggcacc
     2041 gagcactttg acgtgcaggc tttcggcgct gatttcgcct ataccatcga cggtgaaacc
     2101 ctgggcgagg tcgaggatga aaccttctgc gccgacagcg tgattatgac catcagcggc
     2161 gtcaacctcc atcccggtta tgccaagggc aaactcgtca atgcgatcaa gatcgccgcc
     2221 cggatcatcg ccaacctgcc ccaggatgcc ctctcccccg agaccacctc cggtcgcgaa
     2281 ggctatctcc atccccatca cctcaccggc accgtcgagg aggccgtcat caagtttctc
     2341 atccgcgact ttaccatcga ggggctcaag gagaaggaga gcatgctgca gcagtgggcg
     2401 gacaagacca tgaaaaccta ccccaaggcc aaactcgact tgcgggtgga ggaatcctac
     2461 cgcaacatga aatacgagat caacaaggag ccgcgtgtgg tggaacacgc cgtggaggcg
     2521 gtgcggcggg cagggatcac tcccattcaa aacatcatcc gcggcggcac cgatggctcc
     2581 cgcctctcct atcaggggtt gctcaccccc aacatcttca ccggtggaca caatttccac
     2641 tcccgccagg agtggatttc catccaggat atgcaaaagg ccgttgaggt catcattcac
     2701 ctcgcccgca tctgggccga agaagcagcc ggcgtcgcct gacccggccg gcctcagctg
     2761 tcccccgctc ggggataata caataaaaag cgccgcgaca tctcagatgt ctgcggcgct
     2821 ttccgtttgc tagtttccgc tcgggagctg ctgctctcag cagttctggc cttcggcctc
     2881 ctccagaagc tccaggacct ccttgcccgt cttggccttg agcaggcgtt tgcggagaag
     2941 atcctccttg agcaggcgag aaagtttggc caagatcttc aggtaatcgc ccaccgtatc
     3001 gaccggcgtg ccgagcaaaa agatcagccg caccgggtcg tcgtcctcgg ccccgaaatc
     3061 gaccccctcc ttgaggcggg cgaaggcgat gaccagacgg ttgaccgccc gggtacgcgc
     3121 atgaggaata gcgatgccct tgccgatgcc ggtgctgccc agccgttcgc gctcgtagac
     3181 ctcttttttg aactcttcag cattctgaat cagatgcgcc ttggccatgc tctccacgat
     3241 ctctgagatg gcctcatttt tatcatggac ctctatatcc agaaagatag cattcttgtt
     3301 aatgtactcc agtaatcgca aagccttcct ccttgccacc cgtagtgggc cgagcctgtc
     3361 cggaacgatc accgccggac tcgcaaaaaa ggcagcttga aatacaaagc tgcccaaaaa
     3421 aataatattt tacgttcata atttattgct ttattctgta aaagtcaagc ctttcccgag
     3481 tccacaccgc tcagaggctt tgaattttaa gcaaattggt cctggagccc tcgccaaagg
     3541 gatggccgcc gaccaatatg acctgagcgc cctcctgcgg cacagcccgg cgcacgctcg
     3601 gctgcacccg ctgccagagg gtctccatat ccgatgaaaa ggtgatcaac tgcggcacca
     3661 ccccccacat caggctcatc cgccgccagg catcccggtc ggcggtgaag gcgaagatgg
     3721 gggcctgcgg tctctgctgg gagatcagcc gtgcggtttc gcccgaaagg gtgaagacgc
     3781 agatggccgc gatcggcaac tgcttgacaa tggtcgctgc agccgcggcg attgccaggg
     3841 gcccgctttg cggcctctcg atcagccacc gtctggtcgc cgctgcatca ccatggcgtt
     3901 cgctcgcctc cgccgcctca gcgatgcgcg ccatcatggc caccgcctcc acggggtagc
     3961 ggccgaccgc tgattcaccg ctcagcatga ccgcgtcgct gccgtcgatg atagcgttgg
     4021 ccacatcgct ggcctcggcg cgggtgggac gaggattgac ggtcatcgat tccagcatct
     4081 gggtggcggt gatgaccgga cggcccgctt cattggccgc ctcaatcagc tgcttctgca
     4141 gcaccggcac ctgttccggc ggcagctcca cccccagatc gccgcgagct accatgatga
     4201 tgtctacctc ggctaaaatc tcagggagct cggtcagcgc ctcggcccgt tcgatcttgg
     4261 cgacgaccgg aatattcctg cccgctgccg caatcaaggc cttgatctcg cggatgtcct
     4321 ccgcccgacg gacaaaggaa agggtcatca ggtccacgtc ttgcgccagc ccgaaggcaa
     4381 gatcctcacg gtctttctcc gtcagggccg gagcgctcaa ggccacgccc ggaagattga
     4441 tgccctggtg ggcgcgcagc aacccgccgg tgaccacctc ggtggctacc tcccgacccg
     4501 acacctgcac cacgcgcagt tccatgaggc cgtcggagag cagcacccgg tctcccggcc
     4561 tgaggtcatc cggcagggcc gcataactgg tgctcacccg ctccgcatcg ccggggcact
     4621 catcggtggt aatcataaac ggccgccccg cccgcagctc caccgccttg ccgccggcca
     4681 gaagtccggt gcggatcttg ggtccctgca ggtcctggag gatggcaacc ggccggcctt
     4741 tcctctccgc cgcctcgcgc acctgcgcga tgagggcggc gtgctcctga tgtgtgctgt
     4801 gggaaaaatt gatccgcgcg acattcatgc ccgcatcaat cattctttcc aggacggccg
     4861 gctcccgcga tgcgggcccg agcgtcgcaa tgatcttggt tttcctcatc ggctttccct
     4921 cgctaccgtc aggtgatcaa cctctcccca ggagatcaga tgccaggccg cattcctgta
     4981 gaaaaacctc tgaatccccg tattctcgat gctgacccgg aagtaatggc tggcagggat
     5041 gcccaggacg tgacgcagca tgccgatgag gatgcccccg tgggtcacca ccagcagccg
     5101 ccgtcccgcc tctgttctca ccacctcttc cataaaggcg gcaatctcga cctgcttctg
     5161 attgcggctc tgaccgccgg gcagggcaaa atcggcatca tggatgaaaa aacgctccca
     5221 ggcgtcgcca tgcctttctc tggcctcggt aacggtcagg ccctgaaatg tccccatatg
     5281 aaactcgcgc agccgcacat cggtaaccag gtcgatcccc cggctgccgc gtatggtctg
     5341 ggctgtctcc acggcccgct gcaaatcact gctgtagatc ttgtcaatcg gctcagccgc
     5401 cagacgggct gcagccaggc gggcctgctc ccttcccacc tcgctcagcg gcacatccag
     5461 atggccttgc atgcgccggt cggcattgaa ctcggtctcc ccatgccgca gcgcgatcaa
     5521 ctcagtaccc gattccattg ctctcccatc ttttgctttt tccgggcata acataacaag
     5581 ttttttttat atttgcaaaa ggtgtttatc cggccgcggc cgcggcggcg ccatctctgc
     5641 gtaaaataaa aagctatgat ttggaaaata ttttttgtat actacatgaa ttaatacact
     5701 aaccccagtc tcaatcaaat aaggagaagc tcatgcgcag atttatcatc gcggcgctct
     5761 tcacagccct ggtcgccacc ggctgcggcg gcccgaaggg tgcaaaactg gaaaaaggaa
     5821 cgcccatcta tgacctggct gttgaactcg ccaaagccta tcccgctgtg gatcccgatg
     5881 ccaacaaaac cctggtcaag tgcaaatatt tccagatcac cccgggcact ttcttcaccg
     5941 aaatgcagct cgccatgggc aagaatatcg accagatcaa gaccctcgat cccaaacgcc
     6001 tccctgattt tctcaaggtc aacatcgagc gcatgggcga acgcaacctc ctgctggaag
     6061 gggccaagaa agccggcgtc gccatcaccc ccgcccaggt cgacagcacc ctgcagttca
     6121 tctacaccca gaacggcggc gaggaggcat tcatccagcg catcggcagc gacggcatct
     6181 caatcgaaac cgtcaaggcc gatatcgagc gggagatggt catccgcaaa tacttcgaca
     6241 agctctacaa tgaggttaag gtgacggatg aggacctcaa cgcggcctac aaccaggaca
     6301 aaaccgccag cgtgcgccac atcctgctca tgacccaggg caaggatgaa gccggcaagg
     6361 cggatgtccg caaaaagatg gaagagctgc tccgtcgcgc caaagcgggc gaggatttcg
     6421 ccaccctggc cacggaaaac accgaggacc ccggttccaa gaccagcggc ggcctctatg
     6481 aaaactttcc caaaggccac atggtcaaac ccttcgaaga cgccgccttt tcggtgcctg
     6541 tcggcgaaat ctccgacatc attgaaaccc agtacggtta ccatatcctc aaggtggtcg
     6601 atcgcaagaa agagacccgt cctctcgcag aggtcaagag cgaactcgaa cagcagctgc
     6661 tgcgcggcaa gcgccgtgac ctcaacagct ccatcgtcga aaagctgaag aaggattctg
     6721 gctaccagat ctttgtctga tccgtcagac accctgattc accgacagaa cgctgaaagg
     6781 ctccggtccc ccatggggcc ggagcctttt tgataatgcc ctcatctgca caggagcaga
     6841 tcatgccttc gcaaaaaccg gactatacca tccgtcccgc cacagctgcg gatgtgccca
     6901 ccctgctgga actcatccac ggcattgcgg aatatgaaaa gctctcgcat gcggtcgcca
     6961 acagcgagga ggccctgctc gctgccggtt tcgggccgga tccctatttc cgcgccctgc
     7021 tctgcgaaaa ggggaccgaa aagcgggcgg tcggattcgc cctctatttc tatacctatt
     7081 cgaccttcac cggcaaacct tctctctttc tggaggatct cttcgtccgc cccgacgagc
     7141 gcggcagcgg cttcggcaag gccctcttca tggagctggt caaaatcgca cgcgaaaggg
     7201 gatgcggaag gatggagtgg tcggtgctgg actggaaccg gccggctatc gatttctatc
     7261 tcaggctggg ggcgaaggcc atggatgaat ggaccgttta ccggctggat gaagccgccc
     7321 tgcaaaacct cgccggcagg cagggctagg aagcggccgc aatccccgcc agggctccag
     7381 tgctgaaggc aaactgcaga ttgtagcccc cgcagggccc gatgacatcc agcacctcgc
     7441 cggctaacaa gagtccggcc tgctttctgg aggccatggt ccggctcact acctcatcgg
     7501 ctgggatccc tcctgcagag acctgggcaa actcaaagcc ccgcgtccct ctgacctgaa
     7561 gacgggtatt gcaaagggcg ctccacagcg ctgccttttc ccgttcaccc agccttcgca
     7621 tcccaacctc tgccgctacg ccacagctct gaacgacaaa atgggccgcc ttgggcggca
     7681 gcaggccgga aagaacaatc ccgaccggcc actccgaatc tgcaaaacgc tgcagcagct
     7741 cctgcatgag cggctccgcg ccggcgataa aattcaccgc aagctcgagt tccccctttt
     7801 ccccgctgac gagatggctg agatccatcg ccgctggtcc gttcaaaccc cattgcgtaa
     7861 aaatagcgtt gccctcgctc tgcccgagca ggcttttgcc ccgccagagc tggaggccga
     7921 catccatccg caccccctgc atcttgtgaa gccggcgcat gtcggccact atcggagcca
     7981 accccggccg cgcggcgatg actgtatggc cgagacgctc cagctgagca aaaagctctc
     8041 cccgcgcccc cagcttgggc tgcgccaacc ccccggaagc gacgatcaga cgatcgaaac
     8101 gatactcctc ctgtgcgctc agcccccagc cgctcatcgc ggggaagaag gagcgcaccg
     8161 tctcgccgag atgcagttcg atagcggcct gctccagggc tgccgcaaag gcagcgacca
     8221 ctgcggcggc agaacctgaa cgcggataac accagccatc cgccatgacg tggagcggca
     8281 ccgcgatacc ttcgagaaaa gcgaccagct cggcatggcc aaaacccgcc agcacctgct
     8341 ggacaaagcc ggcctcggcg cctgcgtagc ggtccggatc gatccgactg ttggtaatgt
     8401 tacagcgccc gtttccggtc accgccagct tgcgccccac cgtccggttg gcatcgaaca
     8461 gcaccacccg accgccggtc cgtaccgcct gcagggcggc catcatcccg gccggcccgg
     8521 cgccgacaat tccaatctgc aggcctgttt cattcctggg cattctcgtt ctcgctttcc
     8581 ggcaaaccct tccgcccctt catttggggg cgggacgagg tctccatatg atccctgacc
     8641 acgactaaaa gatactgaat cccgcgaccg aagccaacat tttcttcacc ctttccgccg
     8701 ccggatgaca aaatcctttc ctgacgaaat atgtttaaaa tacttgcatt ttgccaacat
     8761 ttactataaa ttcatgattg tgtgctgtac accgtgcgga ccctccggtg ccgatcccgg
     8821 cgaggagcat ccccgcatcc gcaagacacg atgtaaacac caacctgtcc gggctctgtt
     8881 tacgctacaa gtcggccgtt gtggtgtacg ctacatgtac acttttttca gaaagggctt
     8941 ttgctatgga acgaggaacg gtaaagtggt tcaatacgtc taaaggttat ggatttatca
     9001 cgcgagagtc tggcggtgat atctttgtcc acttttcggg catcgttggc gacgggttcc
     9061 gctccctgaa cgggggcgat gcggtggaat ttgaagtgga agaaactcag aagggaccgc
     9121 aggcagttaa ggtgaccaag gtttgattaa ttcataaagc tgaaagccct gaccatgtga
     9181 tggtcagggc ttttttttta gaagacagga tggatcagct tgatcagcca gaaaacgagc
     9241 gcggcggtca gggccgaaaa ggggatagtg accagccagg agatgataat gcgcatggcg
     9301 accccccatt ttatgctcga ggcgtgggtg gtcgagccca ccccgacgat ggccccggcg
     9361 atggtatggg tggtcgatac cggaataccc caatgggtgg cgaaaaagat ggtgaaggca
     9421 cccgaagtct cggcgcaaaa gccgtgcacc ggcttgagct tggtcaactt cattcccatg
     9481 gtcttgacga tgcgccaccc ccccagcgcc gtgcccaggg ccatggcgat ttgacagctt
     9541 aaaatgatcc aggcagtacg cggatcgagc agggagagct gggtgttctc atcaagcgcc
     9601 ccggcggcga ccagcagggc gacgataatg cccatggtct tttgggcatc gttaccgcca
     9661 tggccaaggg aataaagcgc agccgagacc agctgcccct tgcgaaaaag cttgtcgacg
     9721 tcgtaaggcc gccaccggcg aaaaagccag tagaccgcga tcatgatcac aaaaccaagc
     9781 gccattccga tcaggggcga gagcggaatg aaccggactg tcgcccagat cttctcccag
     9841 cggatcaccc ctgtgccctg gaaagcgatg ccggccccgg cgaagccgcc gataagggca
     9901 tgagaggagc tggttggcag ccccagccac caggtgagca catcccagac aatggcgccg
     9961 acgagtccag caaggacgac gtagacaaag gcgggatcac cagcctggat gcggacgatt
    10021 ttggagaccg tgtccgccac ccggggcaca aaaatgaaca tggcgataaa gttgaaaaaa
    10081 gccgcccaga tcacggcatt gcgcggcgaa agcacgcggg tggagacgac ggtcgcaatg
    10141 gaattggccg aatcatgaaa accgttgatg gcatcaaaaa tcagagccat cgcgacggtc
    10201 aggatgacga cgatccaggt cagactcatt ctctatcttt ccgttaaatc agatccgtgg
    10261 tcaggtcagc tcgccgaaat gacaatgccc tcgatgatca ccgccactgt atccgcccgg
    10321 tcggtcgatt tttccagccg ctcaaaaata tccttccatt tgatgatcag gacggcatcc
    10381 tcctcgcgga acaaccgctg caaggcggcg cgcaggacct cgtcgccttg atgctccagc
    10441 tgatggatgg tcatgcaccg tttgttgatg ctctcctgat tcttcaggtc gcgcagctcc
    10501 tgaaccgcca gggcaatctc atccgagcag gccaacagaa tctcggccat ggccaccgac
    10561 tcctcgcgca taatgcggat gtcgtacaag cccatgcgga tcaccccggc atcgatgcag
    10621 tccaaaacat catccatagt cttgataagg cgcaggatat cgggccggtc gatcggggtg
    10681 atgaaggtgc ggttgagggc atcaatgcag cgatgggtga tgtcatccgc ctcgtgctcc
    10741 cagtccttga tcgcctggat ccctacctgg gtatccacct caccgcgcat cagacgcagc
    10801 agttcgcggc tggccgtctg tacaatccgg gcgtggtccg caaaactgtc aaaaaaagca
    10861 tactctttcg gcaacaggct cttgaacatc gtcttctctc cgcaatatca tttgcttccg
    10921 gagaccgcgg cggcgccgct gccccggtca tggatactca atcaggctct cgatgcgctc
    10981 atgcaccaga tcgctcaact cctgcaattc aagccccttg aagttctcgt aggggatggg
    11041 atcaccaacc ttcattttga gcgggcccgg cttgacgatc catcccatcc tgggcttgaa
    11101 tctgaacagc ccggacaggc cgatggggac gatatccacg ccggcgatct gggccagacg
    11161 aaagggcagc ttcttgaaag cgcccaaccg gccggtacac gtacgcgtcc cctccggcat
    11221 aatgatgatc gatttacccc cgcggatctg gctggccgcc ttctcaaggc tgctccagga
    11281 ggagtggatg ctcccacgtt cgatcggtat attgccgatg ctgcgcacaa accagccgat
    11341 caacggccag ctgaaatgtt cagccgcctc aacaccccgc gcgtaattgg gtatgtatcc
    11401 cccgaccagg gggatatcga aaaggctgac atgattgggc atgaaaagat aggtcttgtg
    11461 cggatcgagc ttgtccaccc cctccacggt tgctcgtccg ccgaagacgc ggacaatcag
    11521 gcgcagaaga gcccggcccg gacggttata gatgcgcacc ggcagggtca ccgccatgag
    11581 gatgatcatc agcagggccg gtacggcgat cgccagccca aaaatccaga taaatattga
    11641 ggaaattttt tccagcacac gcgcctcttc cggcaattat ccagcaattt ggagcctgcg
    11701 tccccgcagg cctttatcaa cggctgccct tggcttgatc tcaacagcta cccacggtct
    11761 cacgagggca gcacacccat acgccctcgc ccgggaacag ccgaggtggt ggattaatgg
    11821 ccggaatggt accggcccgg catgaaatgg taatttcaga cgataaatta ccactttcac
    11881 gcttttgttc caaggagaat tatcacaagg aggaggcggg gcccgatcca gacaggggat
    11941 ggatcatccg aaacggccct cgatgtactc ggcggtgcgt ttgtcgagcg gacggatgaa
    12001 gagatcctcg gtgcggctgt gttcgatcag ctcgcccatg agcataaagg cgcattcgtg
    12061 gctgacgcgc ttggcctgcg ccatgttgtg ggtgacgatg atgatggtat agcggccggc
    12121 cagctcgagc atcagctcct ccacctggcg ggtcgcctcc acatccagcg ccgagcaggg
    12181 ctcatccatg aggatgatct ccggcttgag gggcagcagg cgggcgatgc agagcttctg
    12241 ctgctgatcg agcgagaggg atgtggcctt ggacttctgt ttgtccttga ggtccatcca
    12301 cagtccgaca tccgtgaggg cctgctcgac ggcctcgtcg agtgcagaag cattcaacct
    12361 ggccgggctg tgaatatgca ggccgaagac gatgttctca tagaccgtga gaggaagggg
    12421 attggggcgc tgaaaaacca tccccacact cttgcgcagt tcgatgagcg agacatcctc
    12481 atcatagata tttttgccga gcaggcggat ctcacccgtg gtggtgacat tgccgtagcg
    12541 ctcgttgacg cggttgaagc agcgcagcag ggtggtcttg ccgcagccgg aggggccgat
    12601 cagggcggtg atcaatccat tacgcgcctt gaagctgacg gaacgcaggg cctggaattc
    12661 cccgtaccag aggctgagat cgcgggtgac gatgctgtag gcgaggtcgc tcatccgaaa
    12721 atcccgttga cataatcgta ggtcttttgc tgtgccgggc tgttcgaaaa gatgacctcc
    12781 gagcggtcca gctcgatgat atcgccctcg aaaaggaaca tcgtgcggtc ggcgagccgg
    12841 cgggcctgct ggaccagatt ggtgaccaga atgatggtca tctgctggcg cagctccttg
    12901 agcacctcct cgatgcgcat ggtcgtgacc ggatcaatgg cgatggaaaa ttcatcgagg
    12961 cagaggattt ccggctggtg ggagagggcg cgcgcgatgg tgaggcgctg ctgctgtccg
    13021 cccgaaagcc tggttcccag ggtatgcaga cgatccttga cctcatccca gagcgccgcc
    13081 tggcgcaggc agcgctcgac gatatcgtca attgacccct tctgcctgat gccgcccatg
    13141 cgcggcgcga aggcgacatt gtcatagatg gtcaggggca agccgaccgg caggggggcg
    13201 accataccga tcttgcgccg caattcatag acattcctga ccttgccgac ctcctcgccg
    13261 tcgacagtga tcgaaccact gtagcgagcc cctgcagtga attcgagggt gcggttgatc
    13321 gcgcgcagca gactcgtctt ccccgattgc gccggtccga tgaggccaaa aatctcgttg
    13381 cgcaagatct caaaggagat gccgtgcagt acttcatgct gtccgtaaga aagacgcagc
    13441 tcccgcaccc cgattttcgg cgtctgctct accatttctt cttcccgcgc agcttgatgc
    13501 gaaggaggat ggccagggca ttgaaaagca gcaccacccc gagcaggacc acggctgtgc
    13561 cgtaggtcag ggcgtcgggc acatccggaa cctgggtgga gatggtgaaa agatgcatcg
    13621 agagagccat gcactggtcg agaaggccat aggcgaaggg atcaccggtc tgaatcgcct
    13681 tgaaaaagac cgcccccgtg aacatcaccg gcgctgtctc cccggccgcg cgcgagacct
    13741 gaaggatgac gccggtgagg atgccgctga ttgaattggg cagcaccaca ttccggatgg
    13801 tctgccagcg cgtcgccccg acattccagc aggcctcacg gaaggaatgt ggcaccgccg
    13861 ccagggcttc cttggtgctg gcgatgatca ccggcagggt catgatcgcc agggtcagag
    13921 aggcggcgag gatggagcgg ccgatgccgg cgaaaaggac aaaagccccg aggccgaaaa
    13981 gggcgtggac gatgctcggc acaccggcca gattgacgac tgcaagattg atgacccgcg
    14041 tcatccagct ctcgcgcgaa tactcgttca gatagatggc tgcgaaaatt ccgaccgggg
    14101 tggcgatcag cagcgagatc aggaccagat agatcgtgcc cagaagcggg gcccagatcc
    14161 cgccggaacg catgccgtgg atcggattct cgagcagaaa ctccagggag aggatcggcg
    14221 cggccttgta aaagagataa aaaatgatca ggagcagggg caggacaatg atcaccgtca
    14281 tcgtcgtgag cagatatttt attgcgctct cctgccgctg cttgcgcctg gtctgatctg
    14341 tggcttcaaa catgatccgc ttccgctgtt atttcctgcc gatgccgcgc actatgaaat
    14401 ccgctgtgat attgatgaca aaagtgatgg tgaaaaggag gatcccgata atgaagagga
    14461 cctgataatg atccgaatgg acgggagcct cgcccagctc cgcggcgatg gtcgccgtca
    14521 gggtgcgcac cggctcgagc accccgtgcg ggatgcggat ggaatgaccg gtggccatca
    14581 gcaccgccat ggtctcaccc accgcgcggc cgatgccaag catcacggcg gcaagcaggc
    14641 cgttgcgcgc cgccggcagc agcaccctga agatcatctg ccagcgtgtg cagcccaggg
    14701 caacggccgc ctcacgatag gaatcgggaa ccgccttgag ggcgtcctcg ccgatcgaaa
    14761 cgatgatcgg cacactcatc agcgccagga taatcgcgcc gttgaacata ttgagaccga
    14821 tcggggcgtt gaaaagccgg acgatcagag agttcatgat ggtcaggccg atgaatcccc
    14881 acaccaccga gggaatggcg gcgagaagtt cgatgacgat cttgagcccc tccttgagcc
    14941 ggggaggaca aaactcggag ataaagacgg ctgcacccag accgaaaggc acggcaagga
    15001 acatggcaag aaaggagacg ctgaaggtgc ccgtgatcag ggccagggcg ccatagcgta
    15061 catgggtacg tgaggtggga taccactcga tgctggtgaa aaactcgccg gccttgaagc
    15121 cgctgaaaaa atagtcggcc ccctcgcgga agacaaagat gaagatggca aaaacaaaaa
    15181 agatggcact gacgccgcac agccagataa agcgttcgat gagccactcc ttcaacggct
    15241 ccaggtgccg gcggccgggc gtcatggttg atgctcctca ccttgcgccg gagcatggct
    15301 ccccgtacgg gaaagcgaga catagccgga gcgcacgacc agatcctggc cctcggggga
    15361 gtgaatccac tccagataat ccctgagctc cccttcgggc tcgcccaggg tatacatgaa
    15421 gagagggcgg gcgacggggt aaatgccctt ttgggtattc ggcaccgtgg gggcgtaggc
    15481 ggtctcgccg tgcttgcggg cgatacgcag catgcgcacc cggtcggtcg cataccccat
    15541 gccgctgtag ccgatggctc caggcgtccg cgccaccagt tcaaccacat ccttggaacc
    15601 gtgcatgtcg atggtgccga gacgaaaatc acgcgtcttg ccaagcaccg cctcccggaa
    15661 atactcatag gtgccggaat tggactggcg gctgataatg ataattttat cacgcttgcc
    15721 atcgggaggc tgcacgccga gatcgctcca gcgctcgatc ctccctcttt cgccatagat
    15781 ttccgccagg tcttcgaggg tgatctcgtg gaggggattc tgcagattga cgaaaatggc
    15841 cagggcatca tagccgacca gatgctcaac cggatccttg ccggtattct gcagggccct
    15901 ctcccgttca gcaaaagtca tctcccggct gcagttggcg atatccgccg taccgttgat
    15961 cagggaggca atgccggttc ctgatccgcc gccggagacc tccacagaga ccgccggctt
    16021 gattcgcaca tactcctcgg cccaggcctg ggcgagattg accatggtat cggagccgct
    16081 gttctgaatg acctgcttct gccccgtgcc tcgctggccg ggctggcgtc cgcgcagaca
    16141 acccgcagcc agcagcagcg cgagcaggag aggcagaagg ctgaatggtc ctttcatctt
    16201 catcatcgaa tcaacgtcta ttggtttagt ccggagcggt actgatcatg gccgatgatg
    16261 gcctgcacaa gatcccgcag gtgctgggag atttcagtat aggcctggtc gaagcgttca
    16321 acgatcgtct ccgccgatcc cccttcctcg gcagggtcgg aaatcggcca ctcaaagaca
    16381 acgcaattgg agaaatgggc ggggatccgc ttgcgcacct ttgtgttgag cgcgacgatg
    16441 atatcgacgg gcagggtgtc gagaaacacg ctgaaatcct tgggctcgta gccctcgagg
    16501 tcgaagcccc tttccaggag atagcgccgc accgccggct tgatctcccc ggcgggttca
    16561 agccccgcac tggaaaaagc gaaatggccg atcgactggg ccctgccgat cgcctcagcc
    16621 atctggctga gtgaagagtt gtcggcgtcg acgaagagaa tgcggaagag gtcactgtgc
    16681 cggtgcttga taaactggcc tgtggacatg tagatcactt cttcgcagat attcttggcc
    16741 tggtcggtgg ttctttccag gcggcgggcc actgtcatca ggggggccat accctccagc
    16801 gccagttgcc cctcctcctg ccattggatc agcttggcat tgatggcgtt gcgcatctca
    16861 tcggcgatat cctccttgac cataagccgg cgcgcctttt ccacatcatt ttccagaaag
    16921 gcctcgacgc agtcatggaa catggagatc gcgacatcag cgagttcgag aaaatcatcc
    16981 agcgggggga tcggctgcat gggcaaaagg tcgaggacct ggcgggcgat gctctcggcg
    17041 taatcgccga tccgttccag ttcccggata atcttgatgg tggcgaaaac aaagcggaga
    17101 tgaatcgcca ccggctgctg acgcaccaga aactccaggc aaagacggtc cagctcgatc
    17161 tccaggtcat cgacgcagcg atcgcgcagg atgaccgtat aggccagctg ccggtcccgg
    17221 ctcatgaggg cgaggatggc attgcgcagc cccccctcgt cgagcatcgc catctccttc
    17281 gtcttccgct tgagcagggc gatgtcccgt tgcagactct gttcataacg cgattcgcca
    17341 tgcggagtca ccatgattac ctcttctgga tggatcgttc atgaccggcg cgttcagacg
    17401 acgcagaata aatatattaa agtatacttt tgacggtaaa atggcaagct aaatttgcac
    17461 ttggatctca cgggagcccg gccagggcct tttgcagatt gagcctcccg ccggtgacgg
    17521 agagtccgtt gaaggagagg agtggatcga cgctgctcag gagccgctgc ttgagctgca
    17581 gatcggtcag tccgggatcg cgcgccagca gcagtgccgc cgcacccgag acatggggcg
    17641 ccgccatcga ggtgccggaa aaaaccgcat agccgcccgg aaccgtcgaa tagatctcct
    17701 tgccgggtgc ggccagatcc actgtggtgg ccccccagtt ggagcctggg accgcagcca
    17761 gagcgttcga acaggaaaag ccgcagtcgt cggtgttgtc gtcggattcg ccccagagcg
    17821 cccgctgatc gccatggtcc gttgccgcga ccgatacaat gttttcgaga gccaggcagg
    17881 ccgggtactg cggcgagaca tccgcattca cgccgtcatt gccggcagcc gccacgacca
    17941 gaatattatg gttatgggca taatcgatcg cctgctcgag aaagattgat ttgtccggac
    18001 cgccccagct caggttgagg atgcgcgcgc ccagaccaac agcgtattcg attccgtcaa
    18061 tggcaacgcc gacattcccg tccccggagg aatcgagaac ccggatgccc accagacgga
    18121 cctgccagca gactccggtc acgccgagac tgttattgcc cactgcgccg gcaatccccg
    18181 ccacatgggt gccgtgtcca ttttcatcgt agggatcgtt gtccttggca tgaaaatcat
    18241 accccttcca gtcatcgatc aggccgtttc cgtcgtcatc cagcttgttg ccggtcgccg
    18301 gatcattggg gtcgctccag ggatcctcgc ccgggttggt gtaggcgttg gccttgaggt
    18361 cgacatggcg gtaatcgatg ccggtgtcca tgatcgccac cagcacagcg gccgaacccg
    18421 tggtggtcga ccaggcctcg ggcgcatcga tatcggcatc tgccagcccc ccggtctgcc
    18481 ccgtgttgga gagcccccag agcttgccaa aagagggatc gttcggcgtg gccaaggcct
    18541 tgatccggta gttgggttca gccagttcca cctgatcctg cgcgcgcaga tgcgccaccg
    18601 cctcctccac ccccatcccg ccagtgagct tgagccggtg catcgtcaga gagggagaaa
    18661 acgagagcac ccgggcctcc accgcggctg tcaggacgga aacggccttc gcgctggtag
    18721 ccggcttgaa tcgcaccagc acctcatcct cgacgtaagc gggagcagtc gtcgaacgca
    18781 ttccagcact cagggcgatg aagaagatca acaggatgat caaatagagt aagcgtctca
    18841 taacgacctc cggaaagaga ggttatagct gctcaaaggc tcgggaatgg gagaagcggg
    18901 aataatacgt cgcaggaaag tttagtagcc ccagagcgac tcgtagcgga ttttgttgtc
    18961 tttaatacct ttttccagca gctgatcggt cagggcagtg atcatggcag gtggaccgca
    19021 gagaaagtag gtggtattgg cgggatcggg gaggtaggcg gccgccagat ccgcgctcag
    19081 ccagcgccgc tcgcccggcc agggggcctc cggcgccaga ttggtcatgg tgggtatgta
    19141 ggtgaaccgg gcttcagcct gagccagccc ggcgaaaagc tcatgccagg cgctgctatc
    19201 ggggtccgca ttgccgtaaa aaagaaaagt gggggctttg aagccggcgt gcacgccgtg
    19261 catcagcatg ctcctgaagg gggtgatgcc gatccccccg gccagataag ctgcgctctc
    19321 gacctccgga tcatagacaa actgaccgtt cggcccgaga tattcgaaca ggctccccga
    19381 tgccagccgc tgcaaggtct gcttgtagcc gctcccgctc aaacgggtcg caaagcgaag
    19441 ctcgggctcg tgaggcgctg aggcgatggt gaaagtgcgg cgtggcccct tcggatcagg
    19501 aaatagaagc tccggaacct tgagattaag attctgtccc gccagaaagg caaaaccctc
    19561 cggccggtga aaggtcaact cgatagtgcc gcgggcgatc tcctccactc ggctcaattc
    19621 cagcctgtga tccatgactc ctcgaaaata gctcaacctc tgctcttctt gcggcctctc
    19681 tggggtcgac gaaacggagc tccagcctcc atcttcttcg gcctctcctc gtgctccttg
    19741 gacactaaaa caaagtcgat cagcctttca ttccggtcca cacgcgagat gcgcacccgc
    19801 accttgtcgc cgagggcgta aacgcggccg ccccgctgcc cgaccagccg gtagccggag
    19861 ggctcgtaaa tataatagtc gtcctccaga ccggaaatat gcaccagtcc gtcgacgagg
    19921 aattccggca gggtgatgaa gatgccaaag gggacgatgc ggctgataaa gccatcgtgt
    19981 tcactgccga gatggccctc catgtactcg atctgtttga gcttgatcga agcacgctca
    20041 gcctccgcgg cacgcacctc gttttcgctg gcctcccggc attgttccgc cagccgcccg
    20101 gtcagggcct ccatctcctc cacaacgcgg ttggcacagt agcccttgag cagacgatgg
    20161 accatcaggt ccggataacg gcggatgggc gaggtgaaat gggtataatg gctgtaagcg
    20221 agcccaaagt ggccgggatt ctccgggctg tagcgggctt tcatcattgt gcgcagaagg
    20281 gcatcctgga gaacgaaatt tgcggggtgg cggcggatag cggccgccag cctctggaaa
    20341 tagtgggggg tgatcttttt cggcagttcc tgcacgatgc cgaactgagc cagcagctcg
    20401 agcagacccg ccacctcctc gcgctcgggc ttttcatgga tgcgataaac aaagggcggc
    20461 gcctccggca gcccggccgc catctcgact cccacatggc gggccaccgt ctcgttggcc
    20521 atcagcatga actcctcgat gaggcgatga ctctgcagcc gctcgcgcct tttgagagct
    20581 gtcgggtgac cctgctcatc aaggatgacc tgcacctcct ggctctcgaa atcgatgccg
    20641 ccgcgggctt cacggctgcg gatcagcagc gccgccagct cccacatctt ccgcagcagg
    20701 agggagatct catcatcccc gcttccctcg atcatcgcct gggcctgttc ataggtcagc
    20761 cgcctgcggc tgcggatgat gctttcacgg aaagtgtagc ccagcagggc cccagccggg
    20821 gagagctcca tcaggaccga atagcagagc ttctcctccc cctcgaccag gctgcagaga
    20881 cggttggaga ggtgctccgg cagcatcggg atgacccggt ccaccagata gaccgaagtc
    20941 ccgcgcgcct cggcctccgc atcgatcgct ccgcccggcg caacataatg actcacatcg
    21001 gcgatgtgga cgcccagctc gagacggccg ttggcgaggg cgcgcagcga gaccgcgtca
    21061 tcaaagtcct tggcgtccgc cggatcgatc gtgaaaatcg gccagtcacg gcaatcgagg
    21121 cgccgctcat gctcgccgga aggaatcgcc gcccccagcc tggccgcctc cgcctccacc
    21181 tccggcggga actcggaggc gaggctgaaa ccgtggatga tcgagaggac atcgacccct
    21241 ggatcatcgg cgaaaccgag cacatgggtg atgtggccct cgggattctg atggggggag
    21301 ggccagcggt cgatgaccac gaccactttc tgcccctcct cggctcccga cccttccggc
    21361 tcggggataa tgatgtcggt ctggatcttg atgtcatctg gcaccacata gctgtacttg
    21421 cgcccccagc gcagggtgcc gacgatgcgg tcgcgcccgc gctcgacaat ctcgatcact
    21481 tgtccctccg gacgttcccc ctgccggctt gcgaaaagcc gcacccgcac ccggtccctg
    21541 tgcagcgccg agcccatctg tttggtgctc acaaagatat cctcgcgccc gtcatcgcgc
    21601 gtgacaaatc catagccctg tgaattgacc cgcagtatcc cggtgaccac tggcgactgg
    21661 ctggccgagc cgaaacggtt gctgcgcagc ttgagaatgg cgccctcctg cacaagccgg
    21721 cgcagggtct tgcgcagctc cagatactcc tccttgccga tccccagctg ccgggccaac
    21781 tccttggcct tgaagctctt ctgcggctcc cgcgcgaaaa gggccgtcac ttcggcgcca
    21841 agatctcttc ctgcggataa ttttgccatg ctcgtcctca ctgattgtat tgcgtcgccc
    21901 ggaaacgcag cggcccgaag tcgattcggg ccgctggcga ttctctgtta atttttgtca
    21961 aatggacaaa tcatccggat tataggcaat ggcgggatgc ttgtccatct cccggcgaaa
    22021 gattgcgatg atctccgcca gccgctcacg gctcttggcc tcaaagcgca gcaccaggac
    22081 cggctgggta ttggaagcgc ggatcaatcc ccagccgtcg ccgaagagaa cccgggcacc
    22141 gtcgatggtg atggtctcgt ggtttgcact gaagctcttg accatgctct caatgatggc
    22201 gaatttctcc tggtccgggc agtcgacccg gatttccggg gtggagacaa aaagcggcag
    22261 ctcatccacc agcgcggaga agggacgctc actcacggcc aggatgcgca tgaggcggcc
    22321 ggaggcgtag aggccgtcgt cgaagccgaa aaactcgtcg gcgaaaaaca tatggccgga
    22381 catctcgccg gcaaaggggg cgtggatctc cttcatcctg gccttgagca gagagtgccc
    22441 cgtcttgtac atcatgggta ccccgccaag acggctgatc tcttcgggga gcatctgcga
    22501 gcacttgaca tcgaagatga tggtgctgcc gggatgattg gccagcacct cgcggctgag
    22561 cagggccatc aaggcatcgg catagaccag gcggccgcgg tcatcgacga tgccgacccg
    22621 atcggcatcc ccatcgtagc cgatgcccag atccgccttt tccgccagta ccagctggcg
    22681 caggtcctgc atgtatttgg gcacggtggg atcgggcaga tggttgggaa aatgaccatc
    22741 cggatcgcag taaagcggca ccacttcgat ccccagactg ctccacagct ccggggcgat
    22801 gggcccggct gtgccgttgc cggcatcaat caccaccttg agcttgcgcg gaaaggcgaa
    22861 cttggcgcgc atcatctcca tatattcggg gatgatgctc ttctcagtga cgcggccgct
    22921 gctggtgcgc acatagccat tgcggaggat gatctctttc agctcctgaa tggccacgcc
    22981 gtagagggag gccagcccct cgcacatctt gaagccgttg tactcgatcg gattatggct
    23041 gccggtgatc atcacaccac catcggtctg ataatggagg atggcgtaat agaggacggg
    23101 tgtcatcacc tcgccgacca gatagacatt caggccagtg gtgacaatcc cgtcgatgaa
    23161 aagcttggcg agatggggcg aggtcagtcg cacatcatgt ccgatcacca ccagcttgtg
    23221 gccccgtttg gccatccagg tgccgtaggc ccggccgaga ttgtaggcca catcattggt
    23281 cagatcagtc ttgacgatgc cgcgaatgtc atattcgcgg aaaattcctt cattcatgat
    23341 cgcctcccgg ggagaagaga tggacgagtc gttccctgcc ggccaggtcg cgaaaaaact
    23401 ccgctcctgc aaaaccggcc tgtttaaata ttgccacaac ggctgcggac atgccgtcgc
    23461 caaattcgat caaggcttcc ccgcctgttt tgagcagcat tggcagcatc tgcgccaggc
    23521 tgcggtaggc atccagccca tcctcccccc cgagcagggc ctcgcgcggc tcgtgatcgc
    23581 ggatctccgc cgccagcccc gcccaatcgc cgctgcggat atagggcgga ttggaaaccg
    23641 ccaggtcgaa tcccccccgc catgcggaga ggggcgaatt cctgaaatcg tgttccacca
    23701 gttcgacccg gctgctgact ccgtgggctg cactgttgcg ctctgcccac tccagtgcag
    23761 ccgccgagag atcgaccgcg accaggctgg cctccggcat attcttcgcc agggagacgg
    23821 cgatgcagcc ggttccggtg ccgaggtcga tgatgcggac catgccctcc ctggcagcga
    23881 ccgcccgggc ccgctccaga gccttctcga ccagcagttc ggtctccgga cgggggatga
    23941 ggacacccgg cccgacggca aaagggagcg acatgaactc ggtctcgccg agcaggtact
    24001 gcaagggctc atgcgcgacc aggcgcaaaa aaagctgctt gtaatgagcc agctctgccg
    24061 gtgtcagggg ccggtcgaac tgcagataga gatcgacgcg gcgcagctcg agcacatgag
    24121 cgaggagccg ctccgagagc agacggctgt tttcaaaacc ccgagtggcc agatagtcgc
    24181 tgctccactg gatcatccgg agcagggtcc atccctgtat gcttgcgttc tcagccaccc
    24241 gcagccctag ttcatcgtct tgagcatctc ggagcggtca gcaatttgca gctgctcgac
    24301 gaactcgtcg atatcgccct gcagaacggc atccagctgg taaagggtca aaccgatgcg
    24361 atgatccgtg acccggttct gcggaaaatt gtaggtgcgg atcttggcgc tgcggtcgcc
    24421 ggtgctcacc atcgagcgcc gtgaggccgt cagcttggcc tgctcctccg ccagggccag
    24481 atcgtagagc cgggcgcgca gcaccttgag ggccttggcc ttgttcttgt gctgcgactt
    24541 ttcatcctga cagctcacca ccagtccggt gggaagatgg gtaacccgca ccgccgaatc
    24601 ggtcgtgttc acactctggc cgccggggcc ggaagagcga aaaacatcga tgcgcagatc
    24661 gttgggattg atctcgacat cgatctcctc ggcctcgggc aaaacggcga cggaggccgc
    24721 agaagtatga atgcgtccgc tcgcctcggt ctgtggcacc ctctgcaccc ggtgtacacc
    24781 cgcctcgtat ttcatcttgc cataaacatc cctgcccgaa agggcaaaga tcacctcacg
    24841 gaatccgccg attccctggg cgttgatgct cattacctcg agggtccaac cctgtcgctc
    24901 ggcgtagtat tgatacatcc ggaagaggtc gccggcaaaa agaccggcct catcgccacc
    24961 cgtcccggcg cggatctcga tgatcgcatc gcgcgcatcc tgggggtcct tcgggatcag
    25021 cagccacttg agcttctctt ccagttcatc ccgctcggcg ctgagctctt ccatctcggt
    25081 cctggccatt tcagccagtt cgtcatcccc gccctcctca atcacttttt cgtcgcccgc
    25141 gatgtcccgc tcgatcttga ggaggcgctg gtagggttgg acgatttcgg agagctcgga
    25201 ctcctctctg gccagatcgc gaaggaggtg cggcttggcc agcacctccg gatcactgag
    25261 cttcctttgc acctcgaaat aacggacagc gatcgctgca agcttttcct tcacggctgc
    25321 ctcccctatg ccaccatctc gaccggcgac ggacctgcct gcacctcctg gccgagagcg
    25381 cagcgcaggg ccaccatcgc gacctcgatc tgctgctcgt ccggctcgtt ggtggtgatc
    25441 ctctgcagcc agaggccggg gaggatgaag gcacgcacga gagcgaagcg ctgggccctt
    25501 gccgagaact tgatcaactc gtaggagagg ccgccgatga ccggcacaaa ggcgatacgg
    25561 atgagacggt cggcgacagt ctccggcttg cccagaaaca taaagaccag cacgctcacc
    25621 accatgacga tgagaagaaa gctggttcca cagcggggat gaaaagtcgt gaagggcatc
    25681 gccccggccg gggtgagagg ctgattgttc tcatgggcaa aaatggattt gtgctcagcg
    25741 ccgtgatatt caaagacccg cttgatctcc ttccaaaggg agatgaggta gatataggcc
    25801 agaaagagga caagacggat caccccgtca accaaattaa aggcccagcc gctctcgaat
    25861 ccgagcagat cggtgagaat caggggaaga taaaagaaaa aggcgatgcc cagggcgagg
    25921 gaaaagagga tggtcattcc catccagaca tcgttccacc ggttctcgct gcgcccggcc
    25981 ttgcctgtgc ccccgttctc ctccatcatg gcgatatccc ctgaaaaatt gagggccttg
    26041 acgccgagga aaagagattc aatgagcatc accgctcccc ggaaaaccgg cagattcagc
    26101 accttgaacc ttttaacgat cgatttgaac ggggtgcatt tcagaacaat gcccccgtcc
    26161 ggcttgcgca cagccatggc cacgacatct ttgccgcgca tcatcactcc ttcgatgatc
    26221 gcctgcccgc caacggccag agattcctgc tccttcatcc gtggtacccg cttatggttt
    26281 tggattaaaa taaaaaacgg aagtcccgcc ttgtcggcag gatggcttcc gttctgttat
    26341 gtctatggaa aaagctatga ctgcttgttg ggcagattgt acctgcggcg gaactgttcg
    26401 acccggccgg cggtatcaag caatttctgt tttccggtaa agaagggatg gcagctggag
    26461 cagatatcca gcttctcgtc accgacggtg gtgcggacct tgaaggtggc tccgcaggcg
    26521 caggaaacgg tgctcacttc atatttggga tgaataccct ttttcaatgt atggcctccg
    26581 gttcataatg ccgttaaaat tttgcgaaaa taaatataca aaaatgaatc ggcaattgca
    26641 agaaaaacct ttgcggttac atgctcatcg aatcgagaaa ctgcttgttt gatttcgaac
    26701 cctgcatctt ggagaggagg aactccatcg cttcgaccgg tgtcagctcg gccagaagct
    26761 tgcgcagaat ccagacccga ttcagctcct ggtcgccgag cagcagctct tccttgcgcg
    26821 tgcccgagcg gttgacatcg atggagggga agacacggcg gtcggagatg cgccggtcga
    26881 gcaccagttc catattgccc gtgcccttga actcttcgaa aatgacctcg tccatgcggc
    26941 tgccggtgtc gatgagggcg gtggcgatga tggtcagact gcccccctcc tcgatgttgc
    27001 gggccgcgcc gaaaaatcgt ttgggacgat gcagggcgtt ggcgtcgaca ccgccggaga
    27061 ggatcttgcc actgtgcggc accacggcgt tgtgggcgcg cgccagacgg gtgatgctgt
    27121 cgagcagaat gcagacatcc ttgccgtact cgaccaggcg cttggccttc tccagcacca
    27181 tatcggagac ctgcacatgg cgctccgccg gctcatcgaa ggtcgagctg atcacctccg
    27241 ccttgacaga gcgctccatg tcggtcactt cctccggacg ttcgtcgatg agcagcacga
    27301 tcagcttcat ttctggatgg ttggtggtga tggagttggc aattttttgc agcagggtgg
    27361 tcttgccggt cttgggctgg gcgacgatca ggccgcgctg gcccttgccg atcggggtga
    27421 gcatgttcat gatgcgcatc gaaatctcgc ccgctgtggt ctccaggttg atgcgctgat
    27481 gggggtagag gggggtaagg ttgtcgaaaa ggatgatact tttcgcctct tccgggttct
    27541 caaagttaac cgcctcgacc ttgagcaggg cgaaaaagcg ctcattctcc ttgggtggcc
    27601 gaatctgtcc ggagaccgtg tcgcccgtcc tcagcccgaa gcgcttgatc tgtgaagggg
    27661 agacgtaaat gtcatccggc ccaggcagat agttgaaatc gggggagcgg agaaatccgt
    27721 agccatccgg caggacctcg agaactccct cagcgaagat cagcccctcc ttcttggtct
    27781 gctcctccag aatcttgaaa atcaattccg atttgcgcag acctgtgcag cccgtgatgc
    27841 ccatgtcctg agccagcttg ttcagctcgg cgatcttgcg cgccttgagt tcagcaatat
    27901 ccattgacgc ctcttgtcgg taaagttata cgggaattat tgtgcatatt attatgggag
    27961 aaaaacggtg cgcgccatgc aagggatcag acacggtcag cagcaatgag aaactacccc
    28021 ccggggaatc accaccctaa agtcataaag gctgtacagt gcaggagatg atccggaatg
    28081 acgacgagga tggccggcga tgcgcaggaa tggagatcag tgaatggcct cttcgctttc
    28141 cgccatgaaa aagtgcgcct tgttcatatc aaactggacc cgataatccg cgcgcacgct
    28201 cggacgggat tgcacgttca tccgcgccac cagcgactcc ccgccgttgg ccagatagac
    28261 aaaaatctcg ttgcccatgg gctccaccac ttcaacccgg cacagggcgc tcatctccgg
    28321 tatctgggct ggagcgagat catattcgct caaatcttcg gggcgaatgc cgagcacgac
    28381 cggccggccg gtgtgagccg caagcagccc gctgcgggaa tccggaatgc gcagtttcat
    28441 ccccgccgtc cggaagaaaa gcccctcgtc gcggacgatc tcgccgttga tgaaattcat
    28501 cgccgggctg ccgataaagc ccgcaacaaa cttgttgacc ggcgcatcat aaagattcat
    28561 cggggtgtca atctgctgga ccttgccatc tttcattacc acgatgcgat cccccatcgt
    28621 catggcttcg gtctgatcat gcgtgacgta gatcatcgtg gtctcaagcc gtgtatgcag
    28681 tttggagatc tctgtgcgca tctgcacgcg cagcttggca tccagattgg aaagcggctc
    28741 atcaaagaga aacaccttgg gcttgcgcac gatcgctcgg ccgacagcca cgcgctgccg
    28801 ctgcccgccc gatagctgct tgggcttgcg gtcgagaagc ccctcgatgc tcaggatatc
    28861 cgccgcctcc ttgacccgct gttcgatctc atccttggga tacttgcgca gcttcaaccc
    28921 gaaagccatg ttctcaaaaa ctgtcatgtg cggatagagg gcatagttct ggaaaaccat
    28981 ggcgatatcg cggtctttgg gcggcacctg gttgactacc cggtcatcga tgcgaatctc
    29041 cccggcgctg atctcttcca gtccggcgat catccgcagc gtcgtcgatt ttccgcagcc
    29101 ggaaggcccc accagtacga tgaactcctt atcccggata tcgatatcaa cggcatcgac
    29161 cgcgcgaacc tgattgtcaa agatcttgct taccttgtcc agttgaacac tcgccaagat
    29221 tttgctcccg ttctaatgcc gttgtaatag ttgg
//
LOCUS       DAOPWU010000008        28220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_1639_length_28220_cov_5.252867, whole genome shotgun sequence.
ACCESSION   DAOPWU010000008 DAOPWU010000000
VERSION     DAOPWU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 28220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28220
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1639_length_28220_cov_5.252867"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PKM23_01175"
     CDS             <1..557
                     /locus_tag="PKM23_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY90110.1"
                     /translation="VLRQVSATEIPDATSGFRALSREAALQMNVLSRFTYTLETIIQA
                     GKKNLAVSHVPVRTNSKLRESRLFKGNWNYIKRSIATITRIYTMYEPLKMFSYIGGTI
                     FGAGFLIGLRFLYFYITTGGAGHIQSLILAAVLLMIGFQVFIIGLVADLIGFNRRLIE
                     SALYRVRRLELRQGETAREQDKAE"
     gene            554..1624
                     /locus_tag="PKM23_01180"
     CDS             554..1624
                     /locus_tag="PKM23_01180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90111.1"
                     /translation="MKKRILFVAGRELSYSRTHIVYHALLAQGYEVVGCFPPDRSFKH
                     YPGLIWRAVRAARSCDLVLVGFYGQIILPFIRLFTRKPLLFDMYITTLDTMVYDRGKA
                     KPGSLMARLYGFSDWFTYKLADLTVLETRAHIDWFCEVFKVPAGKMRRIFLAVDETQI
                     FPLPAQREEGRFLVHFHGEYAPFHGIKYILRAAHLLREERDIIFQIVGKGITYDQDRA
                     LARELGLDNVRFYDTVPYAELAQMMARADLCLGIFGDNDRVLRVTTNKVVEAIAMGRP
                     LVTARNEPVQELLTHGESAWLIERADPHALAEAILTLRADPALRERLGMEGHRVFQNH
                     CTMPRLGEEFAAIIEEMTAHEG"
     gene            1614..2621
                     /locus_tag="PKM23_01185"
     CDS             1614..2621
                     /locus_tag="PKM23_01185"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNY90112.1"
                     /translation="MKVDFSQLAGKKALITGGLGFIGSNLAQRLVHEGVEVTLLDACL
                     EPYGWNPANITEIHEELLFVKGDVRDRELLEKMVEGKDYIFHLAAQVGREISMADPGL
                     DVDINCNGTIALCRAVAEANPAAKVVYAGSRGQIGEPVYLPVDENHPDHPTDVYGINK
                     LAAEKYLFLYGKIYGFAAVSLRLNNVYGPRCQMQHGYYGILNWFIRNAMQGRPITVYG
                     DGLQTRDYVYVGDVVDAFVRAALVPETDRQIYMIGSGVETLFLDMVKSVIAAVGKGEY
                     VHIPFPPERESIDIRKFVVSCDKMTAATGWRPVVDLAAGVARTVAFYHDRYDEYIKEG
                     E"
     gene            2627..3970
                     /locus_tag="PKM23_01190"
     CDS             2627..3970
                     /locus_tag="PKM23_01190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY90113.1"
                     /translation="MKTDSSTERLQTLQPGARVALFLNYFPALSERFIVNEVAGLLER
                     GLDLRPYALSRPPAGRENSEVPELAQRTFYILPSLQAGKVIGAHFAWLFRHPVRYLRC
                     WSFARRRRTRSESLLGSLWRAAHKQELTKAQRQNVLLNFFLIVPVARQMEEEGFSLIH
                     AQYADSASSLALLASMLLEIPFSFTAHAYDIFTPQVIFEEKLKRARFIVTCTRYNRDY
                     LLQNFGELVGDKILVNYHGLDLTRLQPVARIKQEPPVILSVGRLVPKKGLGVLLHACK
                     ILHDQGVAFKCLIVGDGPERPRLEMFIRLNHLMEQIEITGYMAPSAVIEAYGGASLFV
                     LPCVVEEDGNRDGIPNVIAEAMAMELPVISTTISGIPELVEKGESGLLLDEAEPKLLA
                     QTMLEVLDSPQMQRRLGRAGRARVAAIFDSRRNLDELYNFFQLRLHEIAAGEREA"
     gene            3975..4838
                     /locus_tag="PKM23_01195"
     CDS             3975..4838
                     /locus_tag="PKM23_01195"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNY90114.1"
                     /translation="MITADGHGGLAAVKGLVVHGDDFGLSAAVNRGIWRAWRDGILTA
                     ASLVANGSALDEACALAAGSGLVLGLHLNLTCGRPLLPAERLPSLTDGQGNLPGKWDF
                     WRRALCGRLSAVELTDEINAQFDRLDGLGVRVGHLDSHHHVHLLPAVAAAAAPLMKER
                     GIRWVRRVRGKGEPAGSGAAALLGRLQQRSVALTGRRCEKYYREMAGADDFYGFSWYL
                     HPDPGSFLAGWASGLERGRSEWMCHPADYAPGAPATAAEKRRQRECELLCAPETRRIL
                     EQAGIELMTLE"
     gene            4859..5602
                     /locus_tag="PKM23_01200"
     CDS             4859..5602
                     /locus_tag="PKM23_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY90115.1"
                     /translation="MTEERQKKLSSLSIFFPVYNDWGTITTMAAQAITAAEKITDDYE
                     VILVDDGSEEKTQEILDFLESRFSQIRVIHHEHNRGYGGALRTGFRESRKEFIFYTDG
                     DAQYDVRQLLLLADAMNEQVDVVNGFKIKRHDPYYRVVLGKIYQYTSRFLFSLPIEDV
                     DCDFRLMRRSIFDRFELSSNSGTICVEMIHKMSRTGCRFAEVGVNHFFRSSGKSQYFN
                     FVRLFHVARDYSRLWFRLVVRKEGLHAQA"
     gene            5589..6608
                     /locus_tag="PKM23_01205"
     CDS             5589..6608
                     /locus_tag="PKM23_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017714795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNY90116.1"
                     /translation="MPKREQLPPKFRGANCLITGGLGFIGSTLAHRLVEYDANVLIVD
                     SLIPQYGGNLFNIKGLEKKVQVNIADIRDQYSMNYLVKGQDFIFNLAGQVSHIDSMED
                     PFTDLDINCRSQLSILEACRHNNPEVKIVFAGTRQQYGKPDYLPVDEKHPLHPTDVNG
                     INKMAGEWYHILYNNVYGIRATSLRLTNTYGPRQLMKHGRQGFISVFLRQAIEGKKIR
                     IFGSGKQIRDFNYVDDVVDAFCLAAAFDDCNGNIYNLGGDEPMMLEDLVKLLLETAGG
                     GEYEMVPFPEEKKRIDIGDFYADYRKIRAVLGWHYRTGMKEGLARTIAYYREHQNHYW
                     EQPMN"
     gene            6645..8339
                     /locus_tag="PKM23_01210"
     CDS             6645..8339
                     /locus_tag="PKM23_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90117.1"
                     /translation="MTHRHKENGLIRIESRRRAWWFSAALLLFFALFLLNHVNHFLVP
                     ESDFFDYAEKAVKLRHLEWPDNFKRPPLTSLLIAFISPLIPGKFAELYAAEIISAASA
                     LFSLWFLWHIARWLHPASAPWVVWFWALHASTLKLAIKPKGEFLFTCLLLWAFDRFIR
                     RDWRAYGVGFLSSAVRYEGAMAIGAFGLADLVCGRQRLKTVMLTLLSASFLVIWSLLS
                     SGGDGGGSYADYFAHWQPNLTFFTGFWSELLEFLPLAWYRIGAAAAALLFIAGAVDLW
                     RSDRRAALALAAYLAGFAALHVVWPFPGADYLVMASWAALLFIFAGLFALLKAAGRRL
                     GEHAITGWFARRPLFTALLPAAAAVATLVLVLTTRFPYPQYQPKMGWVALSLVPLALY
                     YWPKRWSAAGTGGFIALMTVALFFSFRLFSASGAGLFDVYYTKAEFRLIGEWFDKSYQ
                     KGDRLLVSQPNIVAYYTRLDARRDFVRLSDLPDLGPQELHAWMRSQGITHAAWMSYSL
                     KAEKKGAWHQWAVKNRSLTALDFLGEAGSAPGFVLIKKLEAGPRRATIYGVLNDYK"
     gene            8353..9453
                     /locus_tag="PKM23_01215"
     CDS             8353..9453
                     /locus_tag="PKM23_01215"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNY90118.1"
                     /translation="MQVPFGDLTRHYLGMKEEIDAAVSRVLASGWFVLGRELSTFEEA
                     FAAHLGVRHAIGVGSGTEALHLALVACGVKPGDEVITVPNTAIPTLSAISFAGATPVL
                     VDVDPLSFCINPDLIEEKISARTRAIIPVHLYGNCCRMDEVMAIAGRHHLAVIEDACQ
                     AHGAEYQGGKAGTFGAYGCFSFYPSKNLGAFGDGGMVVTDDDERARQIVLLRNYGQLE
                     RYYHEIKGFNSRLDEMQAAILSAQLPHLESWNRRRREIAALYSKAISHPAVILPRQQE
                     ESRHVFHLYVVRCQARNALRRHLADHGIGTQIHYPVPCHLQNAYRDLGVPAGSHPVAE
                     RCAAEVLSLPIYPELSDEEVLYVAETINHFEG"
     gene            9494..11278
                     /locus_tag="PKM23_01220"
     CDS             9494..11278
                     /locus_tag="PKM23_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNY90119.1"
                     /translation="MCRWLAERPALLMLLTLAAAAAVRLVLMSVRWINPDEGAHLLDG
                     RLIWQGLHPVIDYGSRQPFYAYLLALFIKLFGVSLTAGRLMPLLASLAIGWLIYRIGA
                     RLYDPLRGWLAAAIYLFLPFTLIWSTVVKTEMPAVLLSLLSAWFLLRALQERGMWSYF
                     ILSGIMAALAYYVRQSTLYMPLATGLFLLLQREPAAGSRWRSLALYAAGYLGLCLLAA
                     FYYWGPLGGRELLFSQINPLNLVWNRLAHTIGILPAQQRVVDTTGFRILDQDPGYTLG
                     AWRDALLFALPAIAAALLSLRRRGREEEGAGRREKFRVFLGLWLVILVLAYAFQSASR
                     GFYTQYFLEALPALLLLGAAWLPAGNAAVPRCGRMLLWARLYGVVFALFRVTWTLPFH
                     WLVVVCMGFLFAAAVTGWKARRRGGRMQPAAMLAAAALFISAAWSGRLLTPRYECVWS
                     PATLAKVESILLREGKPQDAVLSGAMIWTYAAGMQPFDGVSHPTLFFMKYDPGLEERL
                     SADPPAFIILDGYTAKKFSRYWPFIEALLKERYYRVEMVCGSRTTVEIWRLQEPPALP
                     NPDLVLNGGSRPGLMPDAAATVGREVRL"
     gene            11275..12369
                     /locus_tag="PKM23_01225"
     CDS             11275..12369
                     /locus_tag="PKM23_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HNY90120.1"
                     /translation="MKIAIMGIRGIPANYGGFETFIEQMAPRLAERGHEVTVYGRSNI
                     IDYREPFYKGVRIRILPTISHKYLDTVVHTLVCTLCSFFIPYDVVFICNSANAAFCIL
                     PRLAGKPVILNVDGLEWKRQKWNWAGKAFYRISEFLATFAPTAVVSDAREVQSYYQEK
                     FQKESTYIPYGAPETPVRSRETLDRFGLEEREYVLYVSRMEPENNAHRVVEAFEKVKT
                     DKKLVMVGDAPYASDYIAGLRATRDPRIIFTGYIFGQGYRELQSHAYCYVQATEVGGT
                     HPALLEGMGYGNCVLANDVPEHHEVLGQAGCYFSTRERDSLRARLQHLLDHPLDVARY
                     RQVVRERVHARYSWDKVTSDYEALFKKVAG"
     gene            12371..14110
                     /locus_tag="PKM23_01230"
     CDS             12371..14110
                     /locus_tag="PKM23_01230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNY90121.1"
                     /translation="MGTLINTERRKWWTEHRTLLLLAGVGFLLRLLGLGFGDPFRYHP
                     DEMKLVYHAGNLLDYASWSRETFFLLRGYPPLYAFILAAAFIPYILWLMVSGTATSLA
                     TVKVYYYLHPFHFHLVGRWLSLLAGVMTIWVIYAAGRRLYSRRTGLWAAAFWAVTFLP
                     VKNSHFGTVDIVLTLLTTLSFYFSIRIFRDGRMRDYVGAALFAGLAVATKYNAGLIIL
                     PIVAAHFLAPAHDAAGAEEPRRSRFLAALIDRRIWLTGLATIAAFLIACPLPLLDPGR
                     FWESVAGTAEFEQTGKLGSGGSFFSYFTGDHSPGYGFFYDNTFPASLGWGVTLLFMLG
                     ILYLLWRRRREDILILIFPLLLYAVIGQMNYKAMRHLLPLVPFLLLIAAELLSAAAER
                     MKSKRNLLIFNVIVIAAAIAPQLCKSLRYDLALYQVDTRTRMKEWIEQNLPEQSRIGT
                     EEFAPPLLSSLDLNLEIIRRSPDYRRVYNLFGVVPKMFAHGRQRTGDHDARAYVQEQG
                     LDYLVLDSFTRARYEWPLSRQRYPDRVEQRELFYKWVRENCELIVRMEPRNKLQISPV
                     VELYRVKKEKPLP"
     gene            14107..15366
                     /locus_tag="PKM23_01235"
     CDS             14107..15366
                     /locus_tag="PKM23_01235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNY90122.1"
                     /translation="MKRWLEEMESPEMTRRLLWIVGAAGLLLRVVLVVLTQNIGGTAS
                     LDGSTYHSIAMNLLAGRGFSQDGVNPTLFVAPLYPFLLAGTYALAGVHPLLIELLQVL
                     FGTASAWLAWLITRALFGRVPGLIAFVLVLFLPELMVINTYFYTESLFIFLFLLAIWL
                     AMRALERPTWPLLAAAGLAGGLATLTRGVTMLLPLLLLFALLLRRRGIWPSLGRTLVY
                     GLFFALPILPWTLRNYQTFGAAVPVAVGSGDVLWTGNYRPHDGRYSYAKTMAVMDSMT
                     AGLNQVDRDALLTGEALKHIKEQPLAAAGLMVKKFFRFWFWVYEGAPSGMKRQGGGLV
                     QLLLALSYYPILLLFCAGVWLSRRRWRELAFIHLLLGYYVALHVAMLVVPRYRIPVLP
                     LLIFFAAFALYEAGGRSLRERRDAEQL"
     gene            15350..16690
                     /locus_tag="PKM23_01240"
     CDS             15350..16690
                     /locus_tag="PKM23_01240"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNY90123.1"
                     /translation="MQNSFKYVLILFTFCATVAQGITFESIQEVIFGPDLLLVMDIPL
                     LMLYILGRKRIAPAPLARISMVLTYAFFAWSAVGLFFAPNIPFVNQELAANLRTLLIF
                     LAIINFVNTKEDLQYLFLGFALGLFFQGSVAIHQWLRGPVGLYFLGEQPGDWQARGTF
                     VHPSVAGFYFSLMSVLIFRMAVYLRPRFHPLYVVAFFFGVTALYATMNRANWLGFAGS
                     MIIMFGLDFVRGKALTKKARGLLAVIAVVALIGAARYGTIIVERFSDSEKSMMGDHSS
                     SRKSLALDAWRIINEHPLTGVGLNNYKEFVNKETAGLQVVHCSYLLVAAELGYPGGLL
                     FIALIITFLFIGFKTRRSTDPFLYHISSAAVTGVIAFAIGMLPSPDYRNLYVKNHIWM
                     VYGITLVVAKMEHYRRRMLADPRVRAQLAARRKALQEQQEALAARRLPGMQGSF"
     gene            16738..18507
                     /locus_tag="PKM23_01245"
     CDS             16738..18507
                     /locus_tag="PKM23_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNY90124.1"
                     /translation="MRILGFYDDHNAGSAVIEDGKILAAVEEERLSRIKLHNGNEDGE
                     PILSLEMVLKMTNSRPENIDKVAIGIMPAAEMQSYIHHDLFKVQKNHKWILASLFSKS
                     ITWDKYWLWYPYFYNQFRINRVKKLLAKVGLGDKPVEFVDHHRSHAAAAYFTSGRSRA
                     LIVTLDGQGDGQCGQIWLGEEGKLTHLKSISSYHSPGLFYNFITWYLGYKPMRHEGKI
                     TGLAAHGNYDHVAEQFEKMFGFENGEFRYYMAEKQYQHAYPHRTNYDKFRAALPREFE
                     SFSPADVAAGVQVLTERVVARHVDQYLQKTGPIDVVMAGGVFANVRVNQAVAELPNTR
                     SVFIFPAMGDNGIPAGAALHTFYKSMGWPEIKNMPRLDDVYLGPSYSDAEIEAELKKA
                     GLQYTWHDHIAPVIADLLNKGHVVARFDGRMEYGPRALGNRTILYQSTDKSVNDWLNH
                     KLHRTEFMPFAPVTLFEDMEENYHHIAHNEYPARFMTITFNCSESMQKGCPAVVHVDS
                     TARPQLIEEKANPVYYNIVKEYKKLTGLSSVINTSFNMHEEPIVCSPGDAIRAFTQGH
                     LEYLAISNFLVPSPTPEKARSRE"
     gene            18514..19923
                     /locus_tag="PKM23_01250"
     CDS             18514..19923
                     /locus_tag="PKM23_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNY90125.1"
                     /translation="MSSIRLNTLFVSIGQGLRIAMAMLLLPVASRILGPASFGRYNLA
                     TTIMFFTMLADDLGLNMWVTREIAKHKERAQRYFAYAVGLKVVLIPLSLLFVALYLEL
                     GSYDRETVEAVWIFSLYALLTSFRDLAVALFRAFEEMEWESLAQSIEKVLTTALGIAV
                     LLTGGGLKGLAWVFVASAAASTLYSLRPLFRRFVRPAMAFSFREFLPMLRGAVVFGIS
                     VFLTTIYSRIDMVMLSLMKGPDYWGYYAAAHKLIDLSNVVPTVLMIATFPAMARAATP
                     FADHLNQVFTRGFKWLLLLAIPIVPGVLLLARPVVLGFYGAAYAPSIPALQILGCTAA
                     VLFLNIFTAGAFGATNHQGRLVIIQIGGLVVSASLNWLLIPRYAHVGSSIASLVTESA
                     VLTATLILAFRRIVRLTGLVFIRDGVIAAAVMSLGLLLLRSLPPLPLAGIGAALYFAI
                     LFALKTITWQEIWQFKRAK"
     gene            19926..21155
                     /locus_tag="PKM23_01255"
     CDS             19926..21155
                     /locus_tag="PKM23_01255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY90126.1"
                     /translation="MKILYFNYLYDIYGASLGSAIKPMELFAAMRKLGHEVEMVWFKD
                     QPGGPPAGTLKRSARDFFKRHFAFLVHDLRLLLENRKFARIEAEKVAAFRPDIIIARL
                     DLYLFSAIKTARRFGLPLIIEADSPPLYEAVHFQKQYWRIAAIPRRIERHLLRSCDFA
                     VMQSRELQRYFTDAHDLDPARTAVVCNGADIDKFSPREPDRGLAHRFGLTGGPVLGFI
                     GSMSAWHGIDNLLRIVKAVVARYPATRFLLVGSGGGQEKLIRRYLEKHDLTRHVIQTG
                     YVAHVEIPHYLQLMDVVLAPYPNLPFFYYSPVKVFEYMAAGKAVVTTRIGQLARIIRD
                     GWDGVLCPPGDFDAFISAISGLLEDPEIARQMGINARETIIQQHTWKHKALAYEKICQ
                     QVITEHRLRAQEGGSAR"
     gene            21152..22387
                     /locus_tag="PKM23_01260"
     CDS             21152..22387
                     /locus_tag="PKM23_01260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90127.1"
                     /translation="MKILMVHKFYYIEGGAERYYFNLSGLLEHRGHEVIPFAMHHPRN
                     LATPYSSHFVDYFEPDKELARLGLVNGLKSAARVVYNRQARSRIEALIDEVKPDIAHV
                     HGVYHHLSPSVLFSLKKKRLPVIFTLHEYKILCPDYLFLDRAGRVCERCAGRYFWHAT
                     AGRCFRNSLPASGLVTAESYLHRLLGTYRRQVDLYLSPSRFLQGKMIQYGYPEQMVEW
                     LPYTIPMAEYEPCYENDGYFVYVGRLSHEKGVAELVHAMKRVPGAHLKVIGTGRLDEP
                     LQKFVREQGMTNVEFLGYKSGGELREIVRRAMFVAVPSVVYDNSPLAIYEALAHGKPV
                     VGAAIGGIPELIDEGRDGFLFTAGDEESLVQALRRMLECAPQWPEMGRAGRAKAERLF
                     APEAHLQRMEEIYQRFLSR"
     gene            22492..23580
                     /locus_tag="PKM23_01265"
     CDS             22492..23580
                     /locus_tag="PKM23_01265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90128.1"
                     /translation="MTESKHSLLLVVNGFGVGGGELKLLELAALIDRDKYALTIASVG
                     QGGALEEKFRALGCPVHVLPKAFGFDPRLPLRLARLMRACGAELVMSTLFYADIISAL
                     ATFFYRPKALLSWEVITGQLEWYQVLAYRLLAGRFAHVAAVSNSIHPMIRGLRGMKPE
                     RISTIYYGVDLEKYQPRRREEGGEGPVFGTVARLVHQKGHIWLLEAIPQVLARYPGAR
                     WRFAGDGEKRAGLEARARELGIAHAIEFLGSRSDVQELLGSFDIFVLPSLWEGFPNVL
                     LEAMASGLPVIATSVEGSVELVVEGETGRLVPREDAGALAGAMLELAGDAGLRLRMGS
                     SGRKRVEDHFSLGKQVMEFEALFDRFLA"
     gene            23615..24355
                     /locus_tag="PKM23_01270"
     CDS             23615..24355
                     /locus_tag="PKM23_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY90129.1"
                     /translation="MIHGKKVVVVLPAYNAAATLEKTYAEIAFDIVDDVIIVDDASTD
                     ETVKVANRLGIHTVVHPENLGYGGNQKTCYREALARGADVVVMLHPDYQYTPKLVTAM
                     ASLVAIGQYQMVLASRIISGGALKGGMPLYKYISNRFLTLFENILLGQKISEYHTGYR
                     AFSRDLLSDLPLHSCSNDFVFDNEMIAQAVYFGYSVGEVSCPTKYFPEASSINFRRSV
                     KYGFGVLRTALSFRLQKMHLGRYRIFGR"
     gene            24365..25090
                     /locus_tag="PKM23_01275"
     CDS             24365..25090
                     /locus_tag="PKM23_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY90130.1"
                     /translation="MSATLEITRAPRILIVIPAYNEEKSVAAVAREALASAPGVEVLV
                     VDDCSTDRTAAILAAEGINHVSLPVNLGIGGAVQTGFLYAWEKGHDIVMQVDGDGQHP
                     PAQIPQLLQAMREEGLDAVIGSRYAAGSQIVSSWARRLGGGLLGALIRLATGQRVTDP
                     TSGFRAYNRHALEYLRRHYPQEYPEPIIAIDLLRNGFRLGEVPILMKEREHGASSITG
                     LDTLLYMIKVIFAIIIVKIRRRR"
     gene            25092..25490
                     /locus_tag="PKM23_01280"
     CDS             25092..25490
                     /locus_tag="PKM23_01280"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HNY90131.1"
                     /translation="MPDTAHGYLYGNRILYLSIAAGLIFLIFIVQLVRQRKLSERFAL
                     VWMVIPILLILFSSNRALLERLAQLAGIAYAPALMIPIIFGLFTLVSLYFSVKASKSE
                     QQIKKLAQELALLRHAVQQSPPVVRDRESE"
     gene            25526..26938
                     /locus_tag="PKM23_01285"
     CDS             25526..26938
                     /locus_tag="PKM23_01285"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNY90132.1"
                     /translation="MSFGYFFILVAALFFVLPQRQRWLFLLLCNFCYYFFFKAEYLLL
                     LLLAIGVSWWSALRMERHEEQGKRRAWLIFSLVINIGILLTFKYFNLFSRTIMDVSAA
                     VGGSLQMPLLKWALPVGISFYTFSASSYVIDVYRRKLPAERHAGLYAAYVSFFPNIVS
                     GPIERAPHLLPQFHAAREFDGARAASGLRLMLWGFFKKLVVADRLAIYVNAVFGNPDV
                     HSGATSLMASLFFTFQIYCDFSGYTDIAIGVARILGFDLKKNFNRPYFSRSITEFWRR
                     WHISLSSWFRDYLYIPLGGNRVSVPRAYLNLFIVFFVSGMWHGASWTFLIWGGLHGLY
                     AVSSKFSQPWRDRTATRLHIPGGVRRAFALLVTFMLVNFAWIYFRAADLATANRMVAK
                     IFSVDYSRLFIPAPDQFVYSLAAITMLLAVELFQERRSLTAWLDARPLPLRWALYVSV
                     LVIILLMGIFNGSQFIYAQF"
     gene            26907..27884
                     /locus_tag="PKM23_01290"
     CDS             26907..27884
                     /locus_tag="PKM23_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90133.1"
                     /translation="MAASSSTPSSRISRFLARLLLFLLLLAAVDYGLGRVIDHFFRKT
                     LYGENWSKENWLLSRPYDVVCFGSSRTFRHYIPAIMQDSLGLSVFNAGANGQYLYYAY
                     ALEQLVLERYTPQVIVLDLLPNYVTKAESAEEELGRMSSLAPYASHPVVAGLLTSGKF
                     NEKLKLRSRLYRYNSRLLSLAANYFNQPGAMDNGFVYVGKSKFRPLHPFPDDLDENPA
                     VVYDPERIKVLKKFIRSAQEREIQVVLSFSPTATPLSPKIEEILSFYARLAQETGAPF
                     IKIVSAEHPRFYDQTLYSDLIHMNELGGRAFTPLFTAELKKVLSGRHPL"
     gene            27906..>28220
                     /locus_tag="PKM23_01295"
     CDS             27906..>28220
                     /locus_tag="PKM23_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90134.1"
                     /translation="MKTTKNNHWLIGLVLGIILAMGAVLRLIGIRFGLPMAYHNDEWV
                     LVLATRQFFGGDFNPHNFLYPSLLMYIMYAFERLYFLFASGRDDLSTLYTLCRVTVVL
                     FGV"
BASE COUNT         4857 a         9121 c         8344 g         5898 t
ORIGIN      
        1 gggtgttgcg ccaggtctcg gctacggaga tccctgacgc caccagcggc ttccgcgccc
       61 tctcgcgcga ggccgccctg cagatgaatg tgctctcgcg tttcacctat accctcgaga
      121 ccattatcca ggccggcaag aaaaacctgg cggtcagcca tgtgccggtg cgcaccaaca
      181 gcaaactgcg cgaatcgcgc ctcttcaagg gcaactggaa ctatatcaag cgttcgattg
      241 ccaccattac gcgcatctat accatgtacg aaccgctcaa gatgttctcc tacatcggcg
      301 gcacgatctt cggggcgggc tttctcatcg gtctgcgttt tctctatttt tacatcacca
      361 ccggcggggc cggccatatc cagtccctga tcctggccgc agtcctgctg atgatcggct
      421 ttcaagtctt catcatcggc ctggtggcgg acctcatcgg cttcaatcgc cgcctcatcg
      481 aaagcgccct ttaccgggtg cgccggctcg agctccggca gggagagacg gccagggagc
      541 aggacaaggc cgagtgaaga agcgtatcct ttttgtggca ggacgggagc tctcctattc
      601 ccgcacccat attgtttatc atgccctcct ggcgcagggc tatgaggtgg tcggctgctt
      661 cccgccggac cgttctttca aacactatcc tgggctgatc tggcgggcgg tccgcgccgc
      721 gcgcagctgc gacctggtcc tggtcggctt ttacggccag atcattctgc ccttcatccg
      781 gctcttcacg cgcaagccgc tgctcttcga catgtatatc accaccctgg ataccatggt
      841 ctacgaccgg ggcaaggcca aaccggggtc gctgatggcg cggctctacg gcttcagcga
      901 ctggttcacc tacaagctgg cggacctgac tgtgctggag acccgggcgc acatcgactg
      961 gttttgcgag gtattcaagg tccctgccgg caagatgcgg cgcatcttcc tggcggtgga
     1021 tgagacccag atttttccgc tgccggcgca gagggaggag gggcgctttc tcgtccattt
     1081 tcatggcgag tacgctcctt ttcacggcat caaatacatt ctccgggctg cacatctgct
     1141 gcgggaggag cgcgatatta ttttccagat cgtcggcaaa ggcatcactt acgatcagga
     1201 ccgggctctg gccagggagc ttggcctgga taatgtgcga ttttatgata ccgtccccta
     1261 cgccgaactg gcgcagatga tggcccgcgc ggacctctgt ctcggcatct tcggcgacaa
     1321 cgatcgggtg ctgcgggtga cgaccaacaa ggtggtcgag gcgatcgcca tgggccggcc
     1381 cctggtgacg gcgcggaacg aaccggtgca ggagctgctc acgcacgggg agagcgcctg
     1441 gctgatcgag cgagcggatc cccacgccct ggctgaagcc attctcaccc tccgggccga
     1501 tccggcgctg agagaacgcc tcggcatgga ggggcaccgg gtttttcaga atcactgcac
     1561 catgccgcgg ctcggtgaag agtttgcggc gatcatcgag gaaatgactg cacatgaagg
     1621 ttgatttttc acaactggcc ggcaaaaaag ccctgatcac cggcggcctc ggctttatcg
     1681 gcagcaacct ggcccagcgc ctggtgcacg agggggttga ggtaaccctg ctcgatgcct
     1741 gcctcgaacc ctatggctgg aatcccgcca acatcacgga gatccatgaa gagcttctct
     1801 tcgtcaaggg cgacgtccgc gaccgggagc tgcttgagaa gatggtggag gggaaggatt
     1861 acatctttca tctcgccgcg caggtcggcc gcgagatctc gatggccgat cccggcctcg
     1921 atgtcgatat caactgcaac ggcaccatcg cgctctgccg cgcggtggcg gaggccaatc
     1981 cggccgccaa ggtcgtctac gccggcagcc ggggccagat cggcgagccc gtctatctgc
     2041 cggtggatga aaaccatccg gaccatccga ctgacgttta cggcatcaac aagctggccg
     2101 cggagaagta tctctttctc tacggcaaga tttacggctt tgcagccgtc tccctgcgcc
     2161 tcaacaatgt ctacggcccc cgctgccaga tgcagcacgg ctattacggc atcctcaact
     2221 ggttcatccg caatgccatg caggggcgtc cgatcacggt ctacggggac ggcctgcaga
     2281 cccgggatta tgtctatgtc ggggatgtgg tcgacgcctt tgtccgcgct gcgctggtgc
     2341 ccgagaccga ccgccagatc tacatgatcg gttcgggagt ggagaccctt tttctggaca
     2401 tggtcaagag cgtcatcgcc gcggtcggca agggggagta tgtgcacatc ccttttccgc
     2461 cggagcgcga gagcattgat atccgcaagt tcgtcgtttc ctgcgacaag atgaccgcgg
     2521 cgaccggctg gaggccggtg gtggatctgg ccgcaggcgt agccaggacc gttgcttttt
     2581 accacgacag gtatgacgag tatatcaaag agggggagta aaggcagtga agaccgattc
     2641 atctactgaa cggctgcaga cactgcagcc cggcgccagg gtcgcgctct ttctcaacta
     2701 ttttcccgct ctctccgaac gcttcatcgt caatgaggtg gccggactgc tggagcgggg
     2761 actggatctg cggccctacg ccctgagccg cccgccggcg gggcgggaga acagcgaggt
     2821 gcccgagctg gcgcagcgga ccttttatat tttgccctca ctgcaggccg gcaaggtgat
     2881 cggcgcccat ttcgcctggc tcttccgcca tccggtgcgc tatctcaggt gctggtcctt
     2941 cgcccggcgc cgccgcaccc gcagcgagtc gctgctcggc agcctctggc gggcggcgca
     3001 caagcaggag ctgaccaagg cgcagcggca gaatgtgctg ctcaattttt tcctcatcgt
     3061 gccggtggcc cggcagatgg aggaggaggg ctttagcctc atccacgccc agtatgcgga
     3121 ctccgcctcg agcctggccc tgctcgcctc gatgctcctg gagatcccct tctccttcac
     3181 ggcccacgcc tacgacatct tcaccccgca ggtcatcttt gaggagaagc tcaagcgggc
     3241 ccgcttcatc gtcacctgca cccgctacaa ccgggactat ctgctgcaga attttggcga
     3301 gctggtgggc gacaagattc tggtcaatta tcacggactc gacctgaccc gtctgcagcc
     3361 ggtcgcccgg ataaagcagg agccccccgt cattctctcg gtcggccgcc tggtgccgaa
     3421 gaaggggctg ggggtgcttt tgcacgcctg caagatcctc catgaccagg gagtggcctt
     3481 caagtgtctc atcgtcggcg acggaccgga gcggccgcgg ctggagatgt tcatccgcct
     3541 caaccatctc atggagcaga tcgagatcac cggctacatg gcgccgagcg ccgtgatcga
     3601 ggcctatggt ggagccagcc tctttgtcct tccctgcgtg gtggaggagg atggcaatcg
     3661 cgacggcatc cccaacgtca tcgccgaggc catggctatg gagctcccgg ttatctcgac
     3721 caccatctcc ggcattcccg agctggtcga gaaaggggag agcgggctgc tgctcgacga
     3781 ggccgagccc aagctgctgg cgcagacgat gctcgaggtg ctggactcgc cccagatgca
     3841 gcggcgcctc ggccgggccg ggcgcgcgcg ggtggccgcg atctttgatt cccggcgcaa
     3901 tctggacgag ctctataatt ttttccagct ccggctgcat gaaatagctg ccggggagcg
     3961 ggaagcatag cgggatgatc acggcggacg gtcatggcgg cctcgccgcc gtgaagggat
     4021 tggttgtcca tggtgacgat ttcggcctct ctgcggcggt caaccgcggc atctggcgtg
     4081 cctggcgtga tgggatcctg accgccgctt cgctggtggc caacggctcc gccctggacg
     4141 aggcctgcgc cctggcggcg ggcagcggac tcgtgctggg acttcacctc aacctgactt
     4201 gcggaaggcc gctcctgccg gcggagcgac tgccgagcct gaccgacggg cagggtaatt
     4261 tgcccggcaa atgggatttc tggcggcgcg ccctgtgcgg gcgcctctcc gctgtggagc
     4321 tgaccgacga aatcaacgcc cagtttgacc gtctcgatgg actgggggtg cgggtgggtc
     4381 atctcgacag ccatcaccac gtccacctgc ttccagcggt ggccgcagcg gccgctccgc
     4441 tgatgaagga gagggggatc cgatgggtgc ggcgggtgcg cggcaaaggc gagcccgcgg
     4501 gctcgggcgc tgccgccctg ctcggccggc tgcagcaacg cagcgtcgcc cttaccggcc
     4561 ggcgctgtga aaagtattat cgggaaatgg ccggcgccga cgatttttac ggattcagct
     4621 ggtatctcca cccggacccc ggctccttcc tggccggatg ggcctcaggc ctggagagag
     4681 gccgcagcga atggatgtgc catccggccg attatgcccc cggcgcgccg gcgacggccg
     4741 cggagaaacg gcggcagcgc gagtgcgaac tcctctgtgc gccggagacg cggcgaatcc
     4801 tcgagcaggc cggcattgaa ttaatgaccc ttgaatagaa aaagcggaaa ccggagttat
     4861 gactgaagaa cggcaaaaaa aactctcctc actctcgatc tttttccccg tctacaacga
     4921 ctggggcacg attacgacca tggccgcgca agccatcacc gccgccgaaa agatcaccga
     4981 cgactatgag gtgattctcg tcgatgatgg cagtgaggaa aagacccagg agatcctcga
     5041 ttttcttgag agccgcttct cgcagatccg cgttatccat catgagcaca accgcggcta
     5101 cggcggcgcc ctgcgcaccg gttttaggga atcccgcaag gagtttatct tttatacgga
     5161 cggcgatgcc cagtacgacg tgcgccagct cctccttctg gccgatgcga tgaacgagca
     5221 ggtggatgtg gtcaacggct tcaagatcaa gcgccacgat ccctactacc gggtcgtgct
     5281 cggcaaaatc tatcagtaca cctccaggtt cctcttctcc ctgccgatcg aggacgtgga
     5341 ctgcgatttc cggctgatgc gccggagtat ctttgatcgt ttcgagctga gctccaacag
     5401 cggcaccatc tgcgtggaga tgatccacaa gatgagccga accggctgcc gctttgcgga
     5461 ggtgggggtc aaccatttct tccgttcgag cggcaagtcg cagtatttca attttgtccg
     5521 gcttttccat gttgcgcgcg attactccag gctctggttc cggctggtcg tgcgcaagga
     5581 ggggctgcat gcccaagcgt gaacaactgc cgcccaagtt caggggcgcc aactgcctga
     5641 tcaccggtgg cctcggcttc attggcagca cccttgccca ccgtctggtg gaatacgacg
     5701 cgaatgtgct catcgtcgat tcgctcatcc cgcagtacgg cgggaatctc tttaacatca
     5761 aggggctgga gaaaaaggtt caggtcaata tcgccgatat ccgcgatcag tacagcatga
     5821 attatctggt caagggccag gactttatct tcaatctcgc cgggcaggtc agccatatcg
     5881 acagcatgga ggatcccttc accgacctgg acatcaactg ccgctcccag ctctccatac
     5941 ttgaggcctg ccggcataac aatcccgagg tcaagatcgt attcgccggc acccggcagc
     6001 agtatggcaa accggactat ctgcctgtcg acgagaagca ccccctccat ccgaccgatg
     6061 tcaacggcat caacaagatg gccggggagt ggtatcatat cctctacaac aatgtctacg
     6121 ggattcgtgc cacctccctg cgcctgacca acacctatgg gccgcgccag ctcatgaagc
     6181 acgggcgcca gggcttcatc agcgtctttc tccgccaggc gatcgagggc aaaaagatcc
     6241 gcatcttcgg cagcggcaag cagatccgcg atttcaacta tgtcgatgat gtggtcgacg
     6301 ccttttgcct ggcggcggcc tttgatgact gcaacggcaa tatttacaat cttggcgggg
     6361 atgagccaat gatgctcgaa gacctcgtca agctgctgct ggagaccgcg ggcgggggtg
     6421 agtacgagat ggtgcccttt cccgaggaga agaagcgtat cgatatcggc gatttctatg
     6481 ccgattaccg caagatccgc gccgttctgg gctggcatta ccgtacaggt atgaaggagg
     6541 ggttggcgcg gacaatcgcc tattaccgcg agcaccagaa ccactactgg gagcagccga
     6601 tgaactgagc gggggagggg gcgggagagg tagtgatgaa taaggtgacc catagacata
     6661 aagaaaatgg cttgatcagg atcgagtcgc gcagacgcgc ctggtggttc tccgctgccc
     6721 tgctgctgtt ttttgccctt ttcctgctca atcatgtcaa ccattttctc gtccctgaga
     6781 gtgatttctt cgattacgcc gaaaaagcgg tcaagctgcg tcatctggag tggccggaca
     6841 atttcaagcg tccgcccctt acctctttgc tcatcgcttt catctcgccc ctgatcccgg
     6901 gcaaattcgc cgagctctat gccgcggaga tcatctcggc cgcgagcgcg cttttttccc
     6961 tctggttcct gtggcacatc gcgcgctggc tgcatcccgc ctcagctccg tgggtggtct
     7021 ggttctgggc gctgcacgcc tccaccctca agctggcgat caagcccaag ggcgagtttc
     7081 tcttcacctg cctgctgctc tgggcctttg accgctttat ccgccgggac tggcgggcct
     7141 acggggttgg ttttctctcc tcggcagtgc gctatgaggg ggcgatggcg atcggtgctt
     7201 tcggcctggc cgatctggtc tgcggccgcc agcgcctcaa gaccgtgatg ctcaccctgc
     7261 tctccgcctc ctttctggtg atctggagcc tgctctcttc cggcggcgac gggggcggca
     7321 gctatgccga ttatttcgcc cactggcagc cgaacctgac ctttttcacc ggcttctggt
     7381 ccgagctgct cgaatttctg cccctggcct ggtacaggat cggggcggcc gccgccgccc
     7441 tcttgttcat cgccggggcg gtcgatctgt ggcggagcga ccggcgcgca gccctggcgc
     7501 tcgccgccta tctggccggt ttcgccgccc tgcatgtggt ctggcccttc cccggtgcgg
     7561 actatctggt catggcctcg tgggcggcac tgctcttcat cttcgccggg ctcttcgccc
     7621 tgctcaaggc cgcagggcgc cggctcgggg agcacgctat taccggctgg ttcgcccggc
     7681 gtcccctgtt caccgccctg ctgccagcgg ccgcggccgt ggcgaccctg gtcctggtgc
     7741 tgaccacgcg ctttccctat ccgcagtacc agcccaaaat gggttgggta gccctctccc
     7801 tcgtccccct cgcgctctat tattggccga agcgctggtc ggcagcgggg acaggcggat
     7861 tcatcgcctt gatgaccgtg gccctctttt tcagtttccg gctcttctcg gcctcgggag
     7921 ccggcctctt cgatgtctat tataccaagg ccgaattcag actcatcggt gagtggttcg
     7981 acaagagcta tcaaaagggc gaccgcctcc tggtctccca gcccaacatc gtcgcctact
     8041 atacccgcct cgacgcccgg cgggattttg tacgcctctc cgacctcccc gatctcgggc
     8101 cgcaggaact gcacgcctgg atgcgcagcc agggcatcac ccacgccgcc tggatgagct
     8161 acagcctcaa ggcggaaaag aagggggcat ggcatcagtg ggcggtgaaa aaccggagcc
     8221 tgaccgccct cgactttctc ggcgaggcgg gcagtgcgcc cggctttgtg ctgattaaaa
     8281 aactggaggc ggggccgcgc cgcgccacca tttacggggt tctcaacgac tacaaatgag
     8341 atggaaatca ggatgcaggt accatttggc gatttgacca gacactatct ggggatgaaa
     8401 gaggagatcg acgctgctgt cagccgggtc ctggccagcg gctggttcgt tctgggcagg
     8461 gagttgagca ccttcgagga ggcctttgcc gctcatctcg gggtgcgcca tgccatcggt
     8521 gtgggctcgg gcaccgaagc cctccacctc gccctggtcg cctgcggagt gaagccgggc
     8581 gatgaagtga tcaccgttcc caacaccgcc attccgaccc tctcggcgat ctccttcgcc
     8641 ggggccacgc cggtgctggt cgacgtcgat cccctctcct tctgcatcaa tcccgatctc
     8701 atcgaggaaa agatcagcgc gcgcacgcgg gcgatcatcc cggtccatct ctacggcaac
     8761 tgctgccgca tggacgaggt gatggccatc gccgggcgcc accacctggc ggtgatcgag
     8821 gatgcctgcc aggcccacgg cgcggagtat cagggcggca aggcgggcac cttcggcgcc
     8881 tatggctgct tcagttttta ccccagcaaa aatctcggcg ccttcggcga cggcggtatg
     8941 gtggtcaccg atgacgacga gcgggcccgg cagatcgtcc tgctgcgcaa ctatggtcag
     9001 ctcgagcgct attatcacga aatcaagggc ttcaacagcc gcctcgacga gatgcaggcc
     9061 gccattctct cggcccagct gccccatctc gaaagctgga accgccgccg ccgcgagatt
     9121 gccgcgctct actctaaagc catcagccat cccgcggtca tcctgccccg gcagcaggag
     9181 gagagcaggc acgtttttca cctttatgtg gtgcgctgcc aggcgcgcaa tgcactccgc
     9241 cgccatctgg ctgaccacgg catcggaacc cagattcatt accccgtccc ctgccatctc
     9301 cagaacgcct acagggatct gggcgtgccc gccgggagcc atccggtggc cgagcgctgc
     9361 gccgccgagg tgctctcgct gccgatctat ccggagctga gcgacgagga ggtgctctac
     9421 gtcgcggaaa ccatcaacca tttcgagggc tagaagggcg gaggctgaca ggatggcacg
     9481 tatattccat aacgtgtgca gatggctggc ggaacgcccc gccctgctca tgctgctcac
     9541 cctcgctgcc gcggccgcag tgcgccttgt tctgatgtca gtgcgctgga tcaacccgga
     9601 cgagggggcc cacctgctcg atgggcggct gatctggcag gggctgcatc cggtcatcga
     9661 ttacggctcc cgtcagccct tctacgccta cctgctcgcc cttttcatca agctcttcgg
     9721 ggtctcgctc acggcgggcc ggctcatgcc gctcctggcc tccctcgcca tcggctggct
     9781 catctaccgg atcggggcgc ggctctacga tcccctgcgc ggctggctgg ccgccgcgat
     9841 ctatcttttc ctgcccttca ccctgatctg gtcgacggtg gtcaagaccg agatgccggc
     9901 ggtgctgttg agcctgctca gcgcctggtt cctgctgcgc gccctccagg agcgcgggat
     9961 gtggagctat ttcattctca gcggcatcat ggcggcgctg gcctattatg tgcgccaatc
    10021 gaccctctac atgcccctcg ccaccgggct ttttctgctc ctgcagcggg agcccgcggc
    10081 cggttcgcgc tggcggagcc tggcccttta cgcagccggc tatctgggtc tctgcctgct
    10141 ggcggcattc tactactggg ggcccctcgg cggccgcgaa ctcctctttt cccagatcaa
    10201 tcccctcaat ctggtctgga accgcctcgc ccatacaatc ggcatcctgc ccgctcagca
    10261 gcgggtggtc gacacaacgg gatttcgcat tctcgaccag gatccgggct acaccctggg
    10321 ggcctggcgg gatgctctcc tcttcgccct gccggccatc gccgcggccc tgttgagcct
    10381 gaggcgccgc ggccgggaag aggagggggc ggggagaagg gaaaagttcc gggtattcct
    10441 tgggctctgg ctggtgatcc tggttttggc ttacgctttt caatcggctt cgcgcggttt
    10501 ttacacccag tatttcctcg aagccctgcc ggccctgctt ctgctcggcg ccgcctggct
    10561 gccggccggg aatgcggccg ttccccgctg cgggcgcatg ctcctttggg ccaggctcta
    10621 cggggtggta ttcgccctct tccgcgtcac ctggacgctt ccgttccact ggctcgtggt
    10681 cgtttgcatg ggctttctct tcgccgccgc ggtgacaggc tggaaggccc ggcgcagggg
    10741 cggacgaatg cagccggccg ccatgcttgc agccgcagcc ctcttcatca gcgccgcctg
    10801 gtccggccgc ctcctcaccc cgcgctacga gtgcgtctgg tcgccagcca ccctggcgaa
    10861 ggtggagagc atcctcctcc gcgagggcaa gccgcaggat gccgtgctga gcggggcgat
    10921 gatctggact tacgctgcgg gaatgcagcc cttcgacggg gtctcccatc ccaccctctt
    10981 tttcatgaaa tacgaccccg gcctggagga gcgtctcagc gccgatcccc ccgcctttat
    11041 catcctggat ggctacactg caaagaaatt cagccgttac tggcccttca tcgaggcgct
    11101 gctgaaggaa cgttattacc gggtggagat ggtctgcggc tcgcgcacga cggtcgagat
    11161 ctggcggctg caggagcccc cggcgctccc caatcctgat ctggtcctga acggcgggag
    11221 ccggcccggc ttgatgccgg acgctgcggc gacggtgggc cgggaggtgc ggctgtgaaa
    11281 atcgccatca tgggcatccg cggcataccg gccaattacg gcggcttcga aaccttcatc
    11341 gaacagatgg cgccccggct ggctgagcgc ggccacgagg tcacggtcta cggccgcagc
    11401 aacatcatcg attatcgcga acccttttac aagggggtgc gcatcaggat tctgccgacc
    11461 atcagccaca aatatctcga tactgtggtc cacaccctgg tctgcaccct ctgcagcttt
    11521 ttcataccct atgatgtcgt ctttatctgc aacagcgcca atgcggcttt ttgcatcctg
    11581 ccccgtctgg cgggtaagcc ggtgatattg aacgtcgacg gcctggagtg gaagcggcag
    11641 aaatggaatt gggcgggcaa ggccttttac cgcatctccg agtttctcgc caccttcgcc
    11701 cccaccgccg tggtctcgga tgcccgcgag gtgcagagct attaccagga aaagttccag
    11761 aaagagtcga cctatatccc ctacggggcc ccggagacgc cggtcaggag ccgcgagacc
    11821 ctcgaccgct tcgggctgga agagcgcgag tatgtgctct acgtcagccg catggagccg
    11881 gagaacaacg cccaccgcgt ggtcgaggcc tttgagaagg tcaagaccga caaaaaactg
    11941 gtcatggtcg gcgatgcccc ctacgccagc gactatatcg ccgggctgcg cgccacccgc
    12001 gacccgcgca tcatcttcac cggctacata ttcgggcagg gctatcgtga actgcagtcc
    12061 cacgcctact gctacgtgca ggccaccgag gtgggcggca cccatcccgc cctgctggag
    12121 gggatgggct acggcaactg cgtgctggcc aacgacgtgc ccgaacacca cgaggtgctg
    12181 ggacaagccg gctgctattt ctccacccgg gaaagggatt cgctgcgcgc ccggctgcag
    12241 catctgctcg accatcccct cgacgtggcc cgctaccgtc aggtcgtccg cgaacgcgtc
    12301 cacgcccgct actcctggga caaggtgacc tccgattatg aagcactctt taaaaaagtg
    12361 gcaggatgat ttgggaacat tgatcaacac ggaacggcgc aagtggtgga cagaacaccg
    12421 caccctgctg cttctggccg gggtgggatt cctcctgcgc ctgctcgggc tcggcttcgg
    12481 cgaccccttc cgctaccatc cggacgagat gaagctggtt tatcatgccg gcaatttgct
    12541 cgactatgcc tcctggtcca gggagacctt cttcctgctt cggggctatc cccccctcta
    12601 tgccttcatc ctggcggccg cctttatccc ctacatcctc tggctgatgg tgagcgggac
    12661 ggccacctca ctggccaccg tcaaggtcta ttactatctc caccccttcc attttcacct
    12721 ggtcgggcgc tggctctccc tgctggccgg ggtgatgacc atctgggtca tctatgcggc
    12781 cggccggcgt ctttacagcc gccgcaccgg gctctgggcc gctgccttct gggcggtaac
    12841 ctttctgccg gtcaagaatt cccacttcgg caccgtcgat attgtgctca ccctgctgac
    12901 cacgctctcc ttctacttca gcatccgcat cttccgcgat ggaaggatgc gcgactatgt
    12961 cggagccgcc ctcttcgccg gcctggcggt ggcgaccaag tataacgccg gcctcatcat
    13021 tctgcccatc gtggccgccc actttcttgc ccccgcgcat gacgctgcag gggcagagga
    13081 gccgcgccgc agccgcttcc tcgccgccct gatcgaccgc cgcatctggc tgaccggcct
    13141 ggccaccatc gccgcctttc tcatcgcctg ccctctgccc ctcctcgacc ctggccgctt
    13201 ctgggagagc gtcgccggca ctgccgagtt cgagcagacc ggcaaactcg gctcgggggg
    13261 gagcttcttc tcctatttca ccggcgacca ttcccccggc tacggctttt tctatgacaa
    13321 caccttcccg gccagcctgg gctggggcgt gaccctgctc ttcatgctcg gcatcctcta
    13381 tctgctctgg cgccgccgcc gcgaggatat tcttatcctc atctttccgc tgctcctcta
    13441 tgccgtaatc ggccagatga actacaaggc gatgcgccat ctgctgcctt tggtgccatt
    13501 tttgctactg atagccgctg aactcctctc cgccgcggcg gagagaatga agagcaagcg
    13561 taatcttttg atatttaatg tgatcgtcat tgccgctgcc atcgcgcccc agctctgcaa
    13621 gagcctgcgt tatgacctgg ccctgtatca agttgataca cgcacccgca tgaaggagtg
    13681 gatcgagcag aacctcccgg aacagagccg catcggcacc gaagagttcg cccctccgct
    13741 cctcagcagc ctggacctga atctggagat catccgccgt tcgccggact accgccgcgt
    13801 ctataacctc ttcggcgtgg tccccaagat gttcgcccat ggccggcagc gcactggcga
    13861 tcacgacgcc cgcgcctacg ttcaggagca ggggctggac tacctcgtgc tcgacagctt
    13921 caccagggcc cgctacgagt ggccgctttc gcgccagcgc tatccggatc gggtggagca
    13981 gcgcgagctt ttttataaat gggttcggga gaactgcgag ctgattgtgc gtatggagcc
    14041 ccggaacaag ctgcagatca gtcctgtggt cgagctctac cgggttaaaa aggagaaacc
    14101 gctgccttga agcgctggct ggaagagatg gagagcccgg agatgacccg gcgtctgctt
    14161 tggatcgtcg gtgccgccgg gctcctgctg cgggtggtcc tggtcgttct gacccagaac
    14221 atcggcggca cggcctcgct ggacggcagc acctatcaca gcattgcgat gaatcttctc
    14281 gccggccggg gcttcagcca ggacggggtc aatccgaccc tgttcgtggc gccgctctat
    14341 cccttcctgc tggcggggac ttatgcgctg gcgggtgtcc atcccctgtt gatcgagctg
    14401 ctgcaggtgc ttttcggtac cgcctccgcc tggctggcct ggctcatcac ccgcgccctc
    14461 ttcggccgcg tgcccggact gatcgccttt gttctggttc tctttttgcc ggaactgatg
    14521 gtcatcaata cctatttcta caccgagagc ctcttcatct ttctttttct gctggcgatc
    14581 tggctggcga tgcgcgccct ggagcggccc acgtggcctc tcctggccgc ggccggcctg
    14641 gccggggggc tggccaccct gacgcggggc gtgaccatgc tgctgcccct gctcctgttg
    14701 ttcgctctgc tgctgcggcg ccggggaatc tggccgtcgc tgggtcgtac cctggtttat
    14761 ggtctctttt ttgcgctgcc gatcctgccc tggaccctgc gcaactacca gaccttcgga
    14821 gccgcggtgc cggtcgcagt gggatcgggg gatgtgctct ggaccggcaa ttaccggccc
    14881 catgacggcc gttacagcta tgcaaagacc atggctgtga tggacagcat gacggccggg
    14941 ctcaaccagg tcgaccgcga cgcactcctg accggagagg cgctgaaaca tatcaaggag
    15001 cagcctctgg cggccgcggg gctcatggtt aaaaaatttt tccgcttctg gttctgggtt
    15061 tacgaggggg caccgagcgg gatgaaacgg cagggtggag gtctggtaca gctcctcctc
    15121 gccctcagtt actaccccat cctgcttctc ttctgcgccg gggtctggct gagtcggagg
    15181 aggtggcgcg agctcgcctt cattcatctg ctgctgggtt attatgtggc gctgcatgtc
    15241 gcgatgctgg tggtgccgcg ctaccgcatt ccggttcttc ccttgctgat atttttcgcc
    15301 gccttcgccc tttatgaggc cggcggccgt tccctgcgcg agaggcgtga tgcagaacag
    15361 ctttaaatat gtcctgatcc tcttcacttt ttgtgcgacg gtggcgcagg ggatcacctt
    15421 cgagagcatc caggaggtca tcttcgggcc ggatctgcta ctggtcatgg acattccgct
    15481 gttgatgctg tacattctgg gccgcaagcg gatcgctccg gcgccgctgg ccaggatttc
    15541 gatggtgctc acctatgcct ttttcgcctg gtcggcggtc gggctctttt ttgcgccgaa
    15601 tattcccttt gtcaaccagg agctggcggc caatttgcgc accctgctca tcttcctggc
    15661 gatcatcaat ttcgtcaata ccaaagagga tctgcagtat cttttcctgg gcttcgctct
    15721 cggcctcttt tttcagggtt ctgtggccat ccaccagtgg ctgcgcggtc cggtggggct
    15781 ctactttctc ggcgaacagc cgggcgactg gcaggcgcgc gggaccttcg tccatccgag
    15841 tgtggcgggt ttctattttt ccctgatgtc ggttctgatc tttcgcatgg cggtctatct
    15901 gcggcccagg tttcatcccc tctatgtggt cgcctttttc ttcggcgtga ccgccctcta
    15961 cgccaccatg aaccgcgcca actggctcgg tttcgccgga tcgatgatca tcatgttcgg
    16021 gctcgacttt gtccggggca aggccctgac caaaaaggcg cgcggactgc tggcggtgat
    16081 cgccgtggtc gccctgatcg gggcagcgcg ttatggcacg atcatcgtcg agcgcttcag
    16141 tgactcggaa aaatcgatga tgggcgacca ttcatcgagc cgcaagagcc tggccctcga
    16201 tgcgtggcgc atcatcaatg aacacccgct cacgggggtg gggctgaaca attataaaga
    16261 gtttgtcaac aaggagaccg ccggcctgca ggtcgttcac tgcagctatc tgctggtcgc
    16321 ggcggaactg ggctatccgg gcggcctgct ctttatcgcc cttatcatca cctttctttt
    16381 catcggtttc aaaaccaggc ggagcacgga tccctttctc taccatatct cctccgccgc
    16441 ggtcacgggc gtcatcgcct ttgcgatcgg gatgctgccc tcgccggact atcgcaatct
    16501 ctatgttaaa aatcatatct ggatggttta cggaatcacc ctggtggtgg ccaaaatgga
    16561 gcattaccgc cggcggatgc tggctgatcc gcgcgtgcgg gcccagctgg ccgcgcgcag
    16621 gaaagccctt caggaacagc aggaagcact ggcggcgcgg cggttgccgg gcatgcaggg
    16681 ctcgttttag gcgaatcgaa atggagttca tatcaacagc gtaacaagga gagagtaatg
    16741 cgtattctgg gtttctatga tgatcacaat gcaggcagcg ctgtcatcga ggacggcaag
    16801 attctggccg ccgtggagga ggagcggctg tcgcggatca agctgcataa cggcaacgag
    16861 gacggggagc cgatcctcag cctggagatg gttctgaaga tgaccaactc ccggccggag
    16921 aatatcgaca aggtcgcgat cggcatcatg ccggcggccg agatgcagtc ctatattcat
    16981 cacgaccttt tcaaggtgca gaagaatcac aagtggatcc tggccagcct cttcagcaag
    17041 agcatcacct gggacaaata ctggctctgg tatccctatt tctacaacca gttccgcatc
    17101 aaccgggtca agaagctcct ggccaaggtg ggtctgggag acaagccggt cgaattcgtc
    17161 gaccaccatc gttcgcacgc ggcggccgcc tacttcacct ccggccgcag ccgtgccctc
    17221 atcgtcaccc tcgacggtca gggtgacggc cagtgcggac agatctggct gggcgaggag
    17281 ggcaagctga cccatctcaa gagcatcagc agctaccaca gccccggcct cttttacaat
    17341 tttatcacct ggtatctggg ctacaagccg atgcggcatg agggcaagat caccggcctc
    17401 gccgcccacg gaaattacga tcatgtcgct gaacagtttg aaaagatgtt cggcttcgaa
    17461 aacggcgaat tccgctatta catggccgag aagcagtatc agcatgccta tccccatcgc
    17521 accaactatg acaagttccg cgccgccttg cccagggagt tcgaatcgtt cagcccggcc
    17581 gatgtcgccg ctggtgtgca ggtcctgacc gagcgtgtgg tcgcccgcca cgttgaccag
    17641 tatctgcaaa agaccggccc catcgacgtg gtgatggcgg gcggagtctt cgccaatgtc
    17701 cgcgtcaacc aggcagtcgc cgaactgccc aatacccgtt cggtctttat tttcccggct
    17761 atgggcgaca acggcatacc cgccggtgcg gccctgcaca ctttttacaa gagcatgggc
    17821 tggccggaga tcaagaacat gccgcgtctg gatgatgtct atctcgggcc ctcctacagt
    17881 gacgccgaga tcgaggccga gctgaaaaag gcgggcttgc agtacacctg gcatgaccat
    17941 atcgccccgg tcatcgccga cctgctcaac aaggggcatg tcgtcgcccg cttcgacggc
    18001 cgcatggagt acggcccccg cgccctcggc aatcgcacca tcctctacca gtccaccgac
    18061 aagagcgtca acgactggct caaccacaag ctgcaccgca ccgagttcat gcccttcgcc
    18121 ccggtcaccc tgttcgagga tatggaggag aattatcacc acatcgccca caacgagtat
    18181 ccggcgcgtt tcatgacgat caccttcaac tgctccgagt cgatgcagaa gggctgcccg
    18241 gcggttgtgc acgtcgacag caccgcgcgg ccgcagctga tcgaggaaaa agccaatccg
    18301 gtctattaca acatcgtcaa ggagtacaaa aagctcaccg gtctgagctc ggtcatcaat
    18361 accagcttta atatgcacga ggagccgatc gtctgcagcc cgggggatgc catccgcgcc
    18421 ttcacccagg gccatctcga gtatctggcc atctccaact ttctcgtgcc ctcgccaacg
    18481 cccgaaaaag cccggtcgcg cgagtaggac acagtgtcga gcattcgtct caatacgctc
    18541 tttgtctcca tcgggcaggg gctgcgcatc gccatggcga tgctgctcct gcctgttgct
    18601 tcacgcatcc tcgggcccgc ctcgttcggc cgttacaatc tcgccaccac catcatgttc
    18661 tttacgatgc tggcggatga cctcggcctc aacatgtggg tgacgcggga gatcgccaag
    18721 cacaaggagc gcgcccagcg ctatttcgcc tacgccgtcg gcctcaaggt ggtgctgatc
    18781 cccctcagtc tgctctttgt ggccctctat ctcgagctcg gttcctacga ccgcgagacc
    18841 gtggaggcgg tgtggatctt ttccctttac gctttgctca cctccttccg ggatctggcg
    18901 gtcgccctct tccgggcctt cgaggagatg gagtgggagt cgctggcgca gagcatcgag
    18961 aaggtgctga ccacggccct gggcatcgcc gtcctgctga cgggaggggg gctgaagggg
    19021 ttggcctggg tctttgtcgc ctcggccgcc gcgagtacgc tttattccct ccgccccctg
    19081 tttcgcagat tcgtccgccc ggccatggcc ttcagcttca gggaattcct gcccatgctg
    19141 cgcggtgcag tggtctttgg catctcggtc tttctcacca ccatctattc gcgcatcgat
    19201 atggtgatgc tctccctgat gaaggggccg gattattggg gatattacgc agccgcccac
    19261 aaactcatcg atctgagcaa tgtcgtgccg acggtgctga tgatcgccac cttcccggcg
    19321 atggcgcgcg ccgccacccc cttcgccgac catctcaatc aggtcttcac ccggggtttt
    19381 aaatggctgc tgctgctggc gatccccatc gtccccgggg tgctgctcct cgcccgtccg
    19441 gtcgtgctcg gtttttacgg cgccgcctat gccccctcaa ttccggccct gcagattctc
    19501 ggctgtacgg cggcggtgct ctttctcaac atcttcaccg ccggggcctt cggggcgacc
    19561 aaccatcagg gccggctggt gatcatccag atcggcggac tggttgtcag cgccagcctg
    19621 aactggctgc tcatcccgcg ctacgcccat gtcgggtcga gcatcgcttc gctggtgacc
    19681 gaatcggcgg tgctgacggc gaccctgatc ctcgccttca ggcggatcgt gcggttgacc
    19741 gggctcgtct tcatccgcga cggcgtcatc gccgccgcag tgatgagcct cggcctcctt
    19801 ctcctgcgca gcctgccgcc cctcccgctg gcggggattg gcgcagccct ttactttgcc
    19861 atcctcttcg cccttaagac gatcacctgg caggaaatct ggcagttcaa gagagcaaaa
    19921 tgaccatgaa aatactctat ttcaactatc tctacgatat ctacggggcc tccctgggct
    19981 cggcgatcaa gcccatggag ctcttcgccg ccatgcgcaa gctgggccac gaggtggaaa
    20041 tggtctggtt caaggaccag cccggcgggc cgccggcggg caccctcaag cgttcggcgc
    20101 gggatttctt caagcgccat ttcgcctttc tggtgcacga cctgcgttta ctgctggaga
    20161 accgtaaatt tgcccgcatc gaggcggaaa aagtggccgc cttcagaccc gacatcatca
    20221 tcgcccgcct cgacctctat ctcttttcgg cgatcaagac cgcgcgcaga ttcggcctcc
    20281 cgctcatcat cgaggcggac agccctcccc tgtatgaagc ggtccatttt cagaagcagt
    20341 actggcgcat agcggccata ccgcgccgga tcgagcgcca tctcctgcgc agctgtgatt
    20401 tcgcggtgat gcagtcgcgc gaactgcagc gctatttcac cgatgcccac gacctcgatc
    20461 ccgcccgcac cgctgtggtc tgcaacggcg cggatatcga caaattctcg ccgcgggagc
    20521 ccgaccgcgg cctcgcccat cgcttcgggc tgaccggcgg accggtgctc ggcttcatcg
    20581 gctcgatgag cgcctggcac ggcatcgaca atctcctgcg catcgtcaag gcggtggtgg
    20641 ccaggtatcc cgcaacccgc ttcctgctgg tgggctccgg cggggggcag gagaagctga
    20701 tccgccgcta tctggaaaaa cacgacctca cccgccatgt catccagacc ggctatgtgg
    20761 cgcacgtcga gatcccgcac tatctccagc tcatggatgt ggttctggcg ccctatccca
    20821 atctcccctt tttctattac agtccggtca aggtctttga gtacatggcc gccggcaagg
    20881 cggtggtgac cacccgcatc ggccagctgg cgcgcatcat ccgcgacggc tgggacgggg
    20941 tgctctgccc tcccggggat ttcgacgcct tcatctcggc catttcgggc ctgctcgagg
    21001 atcccgaaat cgcccgccag atgggcatta atgcccgtga gaccatcatc cagcagcaca
    21061 cctggaaaca caaggccctg gcctacgaaa agatctgcca gcaggtgatc actgagcacc
    21121 gcctgcgcgc tcaggaaggg gggagtgcgc gatgaagatc ctgatggtcc acaagttcta
    21181 ttacatcgag gggggcgcgg agcgctatta tttcaacctc tccggactgc tcgagcacag
    21241 ggggcacgag gtcatcccct tcgccatgca ccatccacgc aatctggcca ctccctacag
    21301 cagccacttt gtcgattatt tcgaaccgga caaggagctg gcccggctcg gtctcgtcaa
    21361 cggcctcaag tcggccgcgc gcgtggtcta caaccggcag gcccggagca ggatcgaggc
    21421 cctgatcgat gaggtcaagc cggatatcgc ccacgtccac ggggtctatc accacctttc
    21481 gccgtcggtg ctcttctccc tgaagaaaaa gcggctgccg gtgatcttta ccctgcacga
    21541 gtacaagatc ctctgcccgg actatctttt cctcgaccgc gccggccggg tctgcgaacg
    21601 ctgcgccggc aggtactttt ggcacgccac ggccgggcgc tgcttccgca attccctgcc
    21661 tgcctcggga ctggtcaccg ccgaatccta tctgcaccgt ctgctcggga cctatcgccg
    21721 ccaggtcgac ctctatctct cgccgagccg ctttttacag ggcaaaatga ttcagtacgg
    21781 ctatccggag cagatggtcg agtggctgcc ctataccatc cctatggcag agtatgagcc
    21841 ctgctacgag aacgacggct atttcgtcta tgtggggcgg ctatcgcacg agaagggagt
    21901 ggccgagctg gtccacgcca tgaagagagt gcccggagcg catctcaagg tgatcggcac
    21961 cggccggctg gacgagcctt tgcaaaagtt cgtccgcgag caggggatga ccaatgtcga
    22021 gtttctcggc tacaagagcg gaggggagct gcgggagatt gtgcgccgcg cgatgttcgt
    22081 cgccgtgccc tcggtggtct atgacaattc gcctctcgcc atatacgagg ccctggcgca
    22141 tggcaagccg gtggtcggcg cggccatcgg cgggatcccg gagctgatcg acgaaggacg
    22201 cgacggcttt ctcttcaccg ccggggatga ggagagcctg gtgcaggccc tcaggcgcat
    22261 gctcgaatgc gcaccgcagt ggccggagat gggcagggcg gggcgggcca aggccgagcg
    22321 cctctttgcg ccggaggccc atctgcagcg catggaagag atctaccagc gcttcctctc
    22381 gcgttgaacc ccgtgcggcc ggccgccgcg gaacggcgcg tcgccggcgg aggattccgc
    22441 tccgcactcc ggcacttttt tggtatgctt agataaaaga ggattgaaga ggtgactgag
    22501 agcaaacact ccctgcttct ggtggttaac ggtttcgggg tcggcggcgg cgagctcaag
    22561 ctgctcgagc tggccgccct gatcgaccgg gacaagtacg ctctcaccat cgccagcgtc
    22621 gggcaggggg gagccctcga ggaaaaattc cgcgccctcg gctgccccgt ccatgtgctg
    22681 cccaaggcct tcggcttcga tccccggctg cccctgcggc tggccagact gatgcgcgcc
    22741 tgcggggcgg agctggtgat gagcaccctc ttttacgccg acatcatctc ggccctggcg
    22801 acctttttct accggcccaa ggcgctcctc tcctgggagg tgatcaccgg acagctcgag
    22861 tggtatcagg tgctggccta ccgcctcctg gccgggcgtt ttgcccacgt tgccgcggtc
    22921 tcgaattcga tccatccgat gatccgcggc ttgcgcggta tgaagcccga gcggatcagc
    22981 accatctact acggcgtcga tctggaaaaa taccagccgc gccgccgtga ggaggggggg
    23041 gaggggccgg tcttcgggac cgtggcgcgg ctggtccatc agaaggggca catctggctg
    23101 ctggaggcga tcccgcaggt gctggcccgc tatcccgggg cgcgctggcg cttcgccggc
    23161 gacggggaga agcgggccgg gctggaggcc agggcgcgtg agctgggcat cgcccacgcc
    23221 atcgagttcc tcggcagccg cagcgacgtc caggagctgc tcgggagttt tgacattttt
    23281 gttctaccct ccctctggga gggattcccg aatgtcctgc tcgaggccat ggccagtgga
    23341 ctgccggtga tcgccacctc ggtggagggg agtgtcgagc tggtggtgga gggcgagacc
    23401 gggcggctgg tccccaggga ggatgccggg gcgctcgccg gcgccatgct ggaactggcc
    23461 ggagatgcag ggctgcggct gcggatgggg agcagcggcc gcaagcgcgt ggaagaccat
    23521 ttttcgctgg gcaaacaggt gatggagttc gaggctctct tcgatcgttt cctcgcctga
    23581 tccgtccggc catacacagg agaagaaaag taccatgatt cacggcaaaa aagtggtcgt
    23641 cgtgctgccc gcctacaacg ccgcggcgac cctggaaaaa acctatgcgg aaattgcctt
    23701 tgatatcgtc gacgacgtca tcattgtcga cgatgccagc acggatgaga ccgtcaaggt
    23761 cgccaaccgg ctgggcatcc ataccgtggt ccatcccgaa aaccttggct acggcggcaa
    23821 ccagaagacc tgctaccgcg aggccctagc gcgcggcgcc gacgtcgtcg tcatgctcca
    23881 ccccgattac cagtacaccc ccaagctggt gaccgccatg gcctccctgg tcgcgatcgg
    23941 ccagtaccag atggtgctgg cctcgcgcat catctccgga ggggccctca agggcggcat
    24001 gcccctctac aaatacatca gcaaccgctt cctgactctt ttcgagaaca ttttgctcgg
    24061 gcaaaagatc tcggagtacc acaccggcta ccgggccttt tcacgcgatc tgctctccga
    24121 tctgcccctg cacagttgtt ccaacgattt tgtcttcgac aacgagatga tcgcccaggc
    24181 ggtctatttc ggctacagcg tcggcgaggt ctcctgtccg accaaatatt tccccgaggc
    24241 ctcctcgatc aatttccggc gcagcgtcaa atacggtttt ggcgtcctgc gcaccgccct
    24301 gtctttccgt ctgcaaaaga tgcatctggg ccgctatcgc attttcgggc gttgaggcgg
    24361 ggccatgtcc gcaacccttg aaataacgcg cgcgccgcgc atcctgatcg tcatcccggc
    24421 ctataacgag gagaagagcg tcgccgcggt cgcgcgcgag gccctcgcct ccgcgcccgg
    24481 ggtcgaggtg ctggttgtcg acgactgctc caccgaccgc acggcggcca tcctcgccgc
    24541 ggaggggatc aatcatgtct ccctgccggt caatctcggc atcggcggcg cggtgcagac
    24601 cggctttctc tacgcctggg agaaggggca cgacatcgtc atgcaggtgg acggcgacgg
    24661 ccagcatccc ccggcgcaga ttccacaact gttgcaggcc atgcgggagg aggggctgga
    24721 tgcggtcatc ggctcgcgct acgcagcggg gagccagatc gtctcctcgt gggcgcgccg
    24781 cctcggcggc ggactcctgg gggccctgat ccggctcgcc accggccaga gggtgaccga
    24841 cccgacctcc ggatttcgcg cctacaaccg ccacgccctg gaatatctgc gccgccacta
    24901 tccccaggag tacccggaac ccatcatcgc catcgacctg ctcaggaacg gattcaggct
    24961 gggcgaggtt cccatcctca tgaaggagag ggagcacggg gcctcttcga tcaccggcct
    25021 cgataccctc ctctacatga tcaaggtcat ctttgcgatc atcatcgtca aaatcaggag
    25081 gaggcgctag gatgcccgac actgctcacg gctacctcta tggcaaccgg attctctatc
    25141 tctcgattgc tgcaggcctc atttttctca tcttcatcgt ccaactggtg cgccagcgca
    25201 agctgagcga acgcttcgcc ctggtctgga tggtgatacc gatcctgttg atcctcttct
    25261 cctccaatcg cgctttactg gagcggctgg cccagctggc ggggatcgcc tacgcaccgg
    25321 ccctgatgat tccgatcatc ttcgggcttt tcaccctggt cagtctctat ttttccgtca
    25381 aggcgagcaa atccgagcag cagatcaaga aactcgccca ggagctggcg ctgctgcgcc
    25441 atgccgtgca gcagagcccg ccggtggtgc gggatagaga gtccgaataa gccctaatcc
    25501 gaggcccggc atgctattca ataccatgag tttcggttac ttttttatcc tggtggccgc
    25561 gctgtttttt gtcttgcccc agcgccagcg ctggcttttt ctgctgctgt gcaatttctg
    25621 ctactatttt ttcttcaagg cggagtatct tcttctgctc ctgctcgcca tcggcgtctc
    25681 ctggtggagc gcgctgcgca tggagaggca tgaggagcag ggcaaacggc gcgcctggct
    25741 gatcttcagt ctggtgatca atatcggcat cctcctcacc ttcaagtact tcaacctttt
    25801 ttcgcgcacg attatggatg tgagcgccgc cgtgggcggc tccctgcaga tgccgctgct
    25861 caaatgggcc ctgccggtcg ggatctcttt ttacaccttt tccgcctcga gctatgtgat
    25921 cgacgtttac cgccgcaagc tgcccgccga gcggcatgcc gggctctacg ccgcttatgt
    25981 ctcgtttttt ccgaacatcg tctccgggcc gatcgagcgc gccccccatc ttttaccgca
    26041 gttccacgca gcgcgtgaat tcgacggggc ccgcgccgcc tccggcctgc ggctgatgct
    26101 ctggggtttt ttcaaaaagc tcgtcgtcgc cgaccggctg gccatctatg tcaatgcggt
    26161 ttttggcaat cccgatgtcc acagcggggc gaccagcctg atggcctcgc tttttttcac
    26221 ctttcagatc tactgcgatt tttcgggtta taccgatatc gccatcgggg tggcgcggat
    26281 cctcggcttc gatctcaaga agaatttcaa ccgcccctac ttttcccgct cgatcactga
    26341 attctggcgg cgctggcata tctcgctctc gagctggttt cgcgattatc tctacattcc
    26401 tctgggcggc aaccgcgtga gcgtgccgcg cgcctatctc aacctcttca tcgtcttttt
    26461 cgtcagcggc atgtggcacg gggccagctg gaccttcctg atctggggcg gactgcacgg
    26521 cctctacgca gtcagctcca aattcagcca gccctggcgc gaccggaccg ccacacggct
    26581 gcacatcccc ggcggagtgc gccgggcttt cgcgctcctg gtcaccttta tgctcgtcaa
    26641 tttcgcctgg atctacttcc gcgccgccga ccttgccacc gccaacagga tggtcgccaa
    26701 aatcttttca gtcgactaca gccgcctctt cattccggct ccggaccagt tcgtctacag
    26761 cctggccgcc atcaccatgc tgctggctgt ggagctgttc caggagcgtc gctccctgac
    26821 ggcctggctc gacgcgcgcc ccctgcccct gcgctgggct ctctatgtca gcgtgctggt
    26881 catcatctta ctcatgggta tctttaatgg cagccagttc atctacgccc agttctagga
    26941 tcagccgctt cctcgccagg ctgcttctct ttctgctcct gctggccgca gtcgactacg
    27001 gcctgggcag ggtgatcgac cactttttcc gcaaaaccct ctatggggaa aactggtcca
    27061 aggagaactg gctgctcagc cgtccctatg acgtggtctg cttcggttcg tcgcgcactt
    27121 tccgccacta tatccccgcg atcatgcagg acagcctcgg cctttcggtt ttcaatgccg
    27181 gggcgaacgg ccagtatctc tactacgcct acgccctgga gcagcttgtt ctcgaacgct
    27241 acaccccgca ggtgatcgtg ctcgacctcc tgcccaacta tgtcaccaaa gcggagagcg
    27301 cagaggaaga gctcgggcgc atgagttccc tcgcccccta cgcgagccat cctgttgtgg
    27361 ccgggctgct caccagcggc aagttcaacg aaaagctcaa gctgcgctcc cggctctacc
    27421 gctataattc gcgcctgctc agcctggccg ccaactattt caatcagccc ggcgccatgg
    27481 acaacggttt tgtctacgtc ggcaagagca aattccgccc cctgcacccc tttccggatg
    27541 atctggacga gaatcccgcg gtggtctacg atcccgaacg cataaaagtg ctgaaaaaat
    27601 tcatccgttc ggcccaggag cgggagatcc aggtggtcct gagcttctca ccgaccgcca
    27661 ccccgctttc gcccaagatc gaggagatcc tctccttcta tgcgcggctg gcgcaggaga
    27721 cgggggcgcc ctttatcaag atcgtcagcg ccgagcaccc ccgtttttac gatcaaaccc
    27781 tctattcgga tctgatacac atgaacgaac tgggcgggcg ggccttcacc cccctcttca
    27841 cagcggaatt gaaaaaggtg ctctccgggc gccaccccct ttaacccctc ttcaagcgga
    27901 aaaagatgaa gacgacgaaa aacaatcact ggctgatcgg actggtgctg ggcatcatcc
    27961 tcgcgatggg tgcggtcctg cggctgatcg gcatccgttt cggcctgccc atggcctatc
    28021 acaacgacga atgggtgctg gtgctggcga cccggcagtt cttcggcggg gatttcaatc
    28081 cccacaattt tctctatccc tcgctgctca tgtacatcat gtatgctttc gagcggctct
    28141 atttcctctt cgcctcgggc cgggacgacc tctccaccct ctacaccctg tgccgggtca
    28201 cggtggtcct tttcggggtc
//
LOCUS       DAOPWU010000009        27160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_1755_length_27160_cov_5.185464, whole genome shotgun sequence.
ACCESSION   DAOPWU010000009 DAOPWU010000000
VERSION     DAOPWU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 27160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1755_length_27160_cov_5.185464"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PKM23_01300"
     CDS             <1..1130
                     /locus_tag="PKM23_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNY90135.1"
                     /translation="RACCKTASGWMYFGGLNGLIRFHPDSIRVENVPPPVVLRSFRIF
                     NRERLLPRRRSRDAAIVLRHDENFFSFEYTALDIAPPHKHRYAYRLEGIDPDWVQAGA
                     GQTASYTDIDPGSYYFSVKACNADGIWSAPVTLPVRILPAWWMTWWFKAAIALALAAG
                     FWLLYRSRVNHLLEIHRMRFDIAGDLHDEIGSNLSSISVETQLLLASPDLGKEQREQL
                     SDIGETTRETMEAIRDIVWFINPRNDLMEDIQLKLRATAGRLLAGLEWSLELSPQLHF
                     EELDLEVRRNLFLIFKEALTNIVRHSGARTCRIRLFDSSAGYELEIQDDGCGFEAEPA
                     PGESGLLNMRRRAARIKSELQVLSIPGQGTLIRLIVPHRRR"
     gene            1322..4267
                     /locus_tag="PKM23_01305"
     CDS             1322..4267
                     /locus_tag="PKM23_01305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY90136.1"
                     /translation="MTLLGFAKTCCRRNSFRRVLLLLMLLAAGSTLQAGTTGKISGKV
                     TDRKSREPLLGVNIILEGTARGAVTDADGFYQIINIPPGSYTLSARFIGYGSQRLTNV
                     TVSVDRTTQQNLALEQQMIEGQEVTIQAERPAIEMDRTHSAAVVTNRTVEMMPVTTVN
                     EVIELQSGVVSADGELHFRGGRSREVSYLIDGVPVTNAYDQSGGNNVVVENAMIQELE
                     VISGTFNAEYGSAQSGIVNIITKGPAQKFSGSAQIYAGDWLSGRDDIFLGINHFNPLS
                     EKDGQFSLSGPLSSKIGLFLTGRHNRWQSRDWYQRRFNSIDGWRIAAYRRWFQEHNLA
                     ESQTSIAIAIPESLATGDLSEGPLTTGSSTSLSAKIGFYPTAKLNFTYQIFGSLQQSD
                     GIDAGVTAVAGSGSRRYAPDGGGTSKSWSHSHFIALHHFPANNFFYNLNFSYQYNIGE
                     DYYRKDNRIARYPGDEGIQLLSATAQGFSLGTTDGFYTGQSDKNYRTQMLFSGDFNWQ
                     VDKYNFLKAGFIARRHNINTYSWGFKATDQWENNAWPNRGLIDGADYTYEGYWEQLVE
                     YWKNWESINQSERYVAYADSEYTLWRDYNIKPTEAALYLQDKIELGEIIINAGLRFDI
                     FDPNEKYIINPRTEALNLGRPENMKKAALKFQTSPRLGISFPISASGAFHAAYGHFFQ
                     MPSFEYMYNSPLYAMTKLQLEGKRLGNADLKAEKTVAYEIGLQQQLTPAIVADITAYY
                     KDIRNLLGIEQITTLDAVSYTRFINRDYGYTKGITLGISKTGGGWVTGGINYTYAYAN
                     GSSSDPTAIYLIQTATQIGGAPVQFADRKILALNWDQRHTLNLYVNLYRPENWSVGLA
                     GFFNSGMPYSPVFVEKYDINEREYRNAEMKPSRWSVDLKAKKYLRLAGLRSTLFLKID
                     NLFDQLNHNSVHASTGRADQVARLPENQKILLDTLEQEGLFTIAEYDVYPGYFSDPRR
                     IQMGLEINF"
     gene            4310..7777
                     /locus_tag="PKM23_01310"
     CDS             4310..7777
                     /locus_tag="PKM23_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90137.1"
                     /translation="MKRIHPSLLLIRFVMLLGLGLSVTGAASAGEIIRPSGKQPGGLS
                     SWTNTREARTLDKAAASQGNSRYLRWAVHDGNLVTGPTINSGLLAYHYVGAAPLMAWP
                     KGARAVRYIHGGVFYCAAEVKDALSNTIHIVSDNYRRSSAESSADLSHWYAFMPLGRY
                     FNLDQPGSLETPEVYGISEDTGLDGVPYTGDYGEDDGILQKDEDYNRNGELDVSLQNT
                     VGWFAISHRKETWPRDWPVGSFVDDNRREGETRPSLRAGRWNGAFGPYIRATQESYYV
                     MDDRENDEFSYYPFDDKRPWPDGRRGLGFTVTVRDYQWVARLSEDIMISTYDIVNNGK
                     DLEKCVVGMYIDPDMGGSLSGDDAAFDRQEDITYAWSASMLSEEGWPMGYFGFAFLES
                     PGLPFDGVDNDEDGMTDEAQNNDIDDDGDWRSFEDRNSNGVWDSEDLNRNGLLDENED
                     VNGNGRLDYEPLNDDTGSDGLSPESDEYIGPDPNGTEGNGRPDGGEPNFEFTDNSESD
                     QVGLTSFYLRDVDDTMADDEKYWVTEIVPNTFAVRPGYQRDIAWSYGSGYVAFTSEAN
                     SHHYAIALLFGNDEADILRNKRTMQVIYDNDYNFAKPPRTPLLTAIGGDKKVYLSWDD
                     AAEHSRDPIYGLDFEAYYIYRSTDASFGEIKTITDGFGNPLLFKPVAIYDLKDGLKGI
                     HPIRLGSELGADSDLGVSYNMGSDSGLRHNYTDTTVTNGRTYYYAVVSIDKGYHPSFY
                     PSISDREGLLPISPTECSATIQTDPLGRAIWADRNTAIVIPRERPAGWQQPKIGGEGV
                     RHVQGDGTGLVAIRIVNPLAVRDNHTYSLQFRDDGAFFELDSSFTGLTRRIALYSVNG
                     GNSLALYSVDDPNTSEAMADFIYDGFQVLLTNHDVSIDTTYWASGTSALALIDMTQTL
                     SGIALPRDYEIRIMELGAYKPVNIATTTNFQVWDVTDPEAPFQVEYRFTESKSSSVAD
                     RGLLKSGCRVILVNNAVERRQTWKWDFGYPAESDSAAWSMPVKGDLFKVLTRKPFDRY
                     DRFEFTMLGNTVSNRKIAADLEKIYTVPDPYIAASTLERKVINQEEGRGDRRIDFVNL
                     PPECQISIFTSSGRLVRELKHSGDATMSRESWDLRTRDGLEITHGVYFYVVEAPGIGV
                     KRGKLAVIK"
     gene            7855..8883
                     /locus_tag="PKM23_01315"
     CDS             7855..8883
                     /locus_tag="PKM23_01315"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY90138.1"
                     /translation="MRYAISIGLLLLALATTGNAQFETDVSGVGTSAGTFLEIGVGAR
                     AQAMGGAYAALSNDPSGLYYNPAGIVWVKNAQLEVMHNEWLVGTRYDFVGLLLPLPFL
                     NSTLGTNLIMLDYGEQPVRTVERPEGTGELWDARDYTVGLTWALALTDRFSFGLTGKY
                     VSQKIWHETGSAMAIDLGIHYNTMLKGLKLGLAMCNFGNEIGLRGRDLDTSVDPDEKN
                     ENIDRVPAEYKTGTYPLPLLFRFGIAYERELGRFGTALVTVDVNHPSNSTESVNLGAE
                     YGVAGLFYLRGGYANLFEKDRSNGLTLGGGLDYYRRGSMGFRIDYAWSDWGILEESHR
                     FSVGILFP"
     gene            8952..11885
                     /locus_tag="PKM23_01320"
     CDS             8952..11885
                     /locus_tag="PKM23_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90139.1"
                     /translation="MKKTLCILILLTAGMALAQINLPAEGLVGHWTFDNPANLAQATA
                     GNDLVPDKLTGTLNFKAVPGPATGNGAVNVGIGSFYRCYHDIEANGHDPALPDSLPTR
                     VNRFAVVVDVRLPAKEVWYTLFATDNNGDLAESDGDFFIRTDGALGLQATGYSFYKIQ
                     DTEHFFRLVINADLGNYYNYYLDGQLLREGGTRRLDDRFSLDSPDGSNQVLFFGDNDG
                     EDSPIDIAELALYNRPLTPAEIAAMGGYGHFIESETPVSIWNFDKPDSLFWARVGEPL
                     ELMGGVKAAPGPNSSDGAAAVGGGAWLKALVNIPPNGRDNPQNVNRYTLAMDIRQPQR
                     GKFSALLQTNPDNNDDADLFVNPDGRIGSSAIGWSDSTLYVGEWSRVVMTCNAGDTTS
                     SLILYVDAKSCLKVAELATDGDLSLAARTGLNRMLFFADENNEDAPLEVANITLYNRP
                     LSEKEVIALGKYEHLRSKEVTPALHTVKFRIADEIQYAAIPHHPDFDFGAGTSFTIEC
                     WMRADLPYEGDPALISNKDWDSGANPGWVLSARGDDWKFNIADKTGLRFDAAITTPDV
                     NDGFWHHLAVIVDRSKQEYVLITDNSVTVAQSMVSGEGVALGDIDTGLPIGIMQDGTG
                     NYAWGYKMPGAVDEVRIWNSALSPETVKKWAHRLVTAEHPDYASLITYLKFDDASGRV
                     VTDASGRGHHAELKGQIEWQVSYAPLADAVADGLKDLSGIWGGQKAGASGGMTLTADF
                     KFNLAKLPAAAASGPVQEADIESYLNEQYALSGHNSLSGVVKTELPAIVLARYGRVWY
                     LDASASYTQKTKVLFTFNETPGAAAGYVLLNRAGASGNFAALGTAAAVDGNNVIFANL
                     TLKDGSYYTLGTKDETASPLGTLSGITGEGGSNFTYELKNAWPNPFNPRTTIAYSLAK
                     SSQVRLTVYNQLGQAVANLVDGKQESAGRYQVVWDARSMPSGLYFFRLEAGAFTKTGK
                     MVLVK"
     gene            11960..13483
                     /locus_tag="PKM23_01325"
     CDS             11960..13483
                     /locus_tag="PKM23_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90140.1"
                     /translation="MALLFIAIFFLAAGCGRKKQPRDPAVARVGDRVITVSEFRSNYE
                     FGMPFLKKGPDPKSAYLDYMIREKLLALEGYRLGFDRHPRVRENEAALMPELLIEELF
                     DHEIRRKITVTTEEIHEGITKAKVSWKFRYWFEPNRADALAVRTAMEEAGYARVVAEL
                     LESNPEIRLEPADFESGYLTWLEIDPAILAAIKDLPVGEISEPLFINGAWVLFQITDI
                     RREAFTENAFREGAESMRQVLFYRKAQTAVRRYVDSLMTPLDVRTKAESFRLLADALA
                     EWTALEPAERGEVLAVAAKAGPEQPHLRKLNNHLDAPLTEFAGGRWTISDFLQRFDPA
                     SIKAPAGDLHAFRKSINQRLALTLRDHYLAGEARKLKLHKSPRLQHSLQQWRDKWVYD
                     EARSHFLRSVMVDSAATRTFFQQNLARYRLHKRKEPLFTEFSEEAKRDAWLQKAREML
                     NGEAAALQRRYPVTIHRAILDTIHVTAAADNKWMSVQLFKSGSQRMAAPIADPAWGL"
     gene            13547..14212
                     /locus_tag="PKM23_01330"
     CDS             13547..14212
                     /locus_tag="PKM23_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNY90141.1"
                     /translation="MKSDRGSTPAGPIDLWLIEDNPRYRKTIAGLMEHTGGIHCSQAM
                     ATCEEAIRLAESESPPQVILLDIGLPGMNGIQGIEVFKSLLPTTHIIILTIYDDNNKV
                     LEAICAGASGYLLKDSPPEKIIESIQEVMAGGAPMNMQIAAKVLEMFREHYPKKSDYG
                     LTEREKEILQHLVSGLTKQQIAGKLFLSFHTVNTHIKNIYTKLHVNTRSGAVSKVYKE
                     KLL"
     gene            14396..16627
                     /locus_tag="PKM23_01335"
     CDS             14396..16627
                     /locus_tag="PKM23_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90142.1"
                     /translation="MKNSYLYRLTLLFALFSALAVFAQAPDPAGWWKFDDPADLLKPE
                     AGFGLPLELVGTHQSVDGPAAGNGAVKIGIGSHYKMMHGIAPNGGGSFVNEFTLMFDF
                     RVEQLNKWYTLFQTAMENNNDGDCFIDKEGNIGTGSTGYTDYAVIPNEWYRLVISVKN
                     GTHYRYYLDGLLAFDADIQDIDGRYGLEPLLLLMADDDGDDGSIEIAEVGIWSTALTS
                     WQVQSLGGYDHQQPLDPMQPAGRWKFDNVDNLAEGYFGKDLVLTGSELAVEGPSTQNK
                     AVRIGPGSYYIMEPGIAPNGGGSYINEFSMQFDFKVPEIGKWHAFLQTNTSNGNDGDL
                     FVNADGQIGVASTGYSDLKINAGEWYRLIISVRNGEFFHYFLDGKLLFEGLKQDLDGR
                     FGLEDKVLLFADNDGEDGEIDIADVAFWGRALADTEVVKLGGYAHESKKVAGSWKFDD
                     AGDILKAEAGLGLPLELVGSHEVIEGPAPGNGAIRIDKGSHYKLTHGIAPNVGGLLVN
                     EYTIHADIRIPVLSGWHTLYQTTPANNNDGDCFIKSSGVIGTGDTGYSTDAILANEWY
                     RVVISVKNGTHYQYYWDGQLVYEATVQGIDSRFALEPVLLMFGDDDGDDGSIDCAEIT
                     VWNYALNADEVAALGGFGHVVGVEGNEAGAIVTRYEMDQNYPNPFNPETSISYALVKS
                     GHVRLEVFNMLGQKMATLIDHQQESGRHSLRLSGAGWPSGVYFYKLSANGFTQTKKMF
                     LCK"
     gene            16653..19163
                     /locus_tag="PKM23_01340"
     CDS             16653..19163
                     /locus_tag="PKM23_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNY90143.1"
                     /translation="MKKTILLLLLIAAATSGQIPHPIGWWKFDDPASLTRAEAGFGRN
                     LEAVGSAVTAVSGPDSGNGAVRIGTGSYFKLDHDIPGNGDGIYLNEYTLAFDFKVPAL
                     GVWYCFFQTNTANSNDGECFINPYGNVGVGATGYSGASIKPGEWYRLILSVKNGSFFQ
                     FYLDGVPFFSGATQIRDGRFSIENPLLLFADENGEDGAIDCAEVAIWDRPLTAVEVKL
                     LGGFGHTFHYDLMTRVPYLQAPTPTSIQVCWHDSSAVPSRVEYGLSPALGESAAASQE
                     QLSGPYRWHAAKLAGLQPDTEYFYRVVSGDGASPLCTFRTLPEPAKAGKLRMLLLSDT
                     HSGDSTMVNRVVRAARAKIGALYGEDLHNQLNLVFHSGDLVVSGGVINQYTDQYFRPM
                     AQLSSSVPFMTVTGNHEGESPYYYQYMKYDDHSLLAPPSAYAERMWELKAGNTLFIGL
                     NTNIVSAAGALQKALLDGRLQKAEQDPAIDFVFIFFHHLPWSELWVEGVTNDAGSNFI
                     RRELFPVIRKYTKVQQLTYGHTHAFERGTVESPMPDGDFRIVCAGGGGGATDNWGEFQ
                     NIDHPFIHVAYDHYFFQILEIDVPNHSFEISMYSLGNQFKSREAQLMDRWYRKVSQHG
                     PDTPVIETPIMQESALVLKSSPFSGPDSLMSVQIQIAEGAEFSSPKIDTLVHWRNIYG
                     VDANYEPVDKNAGIDLTRTAFSRARFQTGKSYACRVRYRDHNLKWSGWSNATSFNVEA
                     SVGLGDESPGRYELAQNYPNPFNGSTIIAYQIPNPAHVSLKLYNLLGQDVRTLVDTQM
                     AAGMHRVELDSRGLPSGLYIYELACDAFLVRKRLNLAK"
     gene            19410..21443
                     /locus_tag="PKM23_01345"
     CDS             19410..21443
                     /locus_tag="PKM23_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY90144.1"
                     /translation="MTTDSGAKPHFVKRWLNRLISAFRLSSEDKELTRTVIDLENIRR
                     VFYLSLLAIPTSACYFIFFLIKSAGETGVTYQWRHAIWICHAILLVSFIVINLVIYFF
                     AYKPGKNSPLALACIHITAVILLLEGAALAAADQLVTDNITAYLITCLITGLVLLTPP
                     RFALLYYLISFTVFYFAISLTQKNPAILISNQINGLTAAALGFCLSIILWRGYLIRVR
                     QSRVIEKQNSELKTALDQVHSQKEDVEQLSRIGRDITSSLSIENIIHTIYENVNTLMD
                     ASVFTIGLHNPGKGVLEFPATIEDNRSLPPFSVALSDETHLAVRCFNHHREVVINDCS
                     AECGEYGGALTSSLTGEPPLSFLYLPLWKKDKVIGVISAQSRVRNVYDDYHVNMLRNL
                     AAFSAIALDNAEAYHRLSALLEELRSTQEKLVTQSKLAALGALTAGIAHEIKNPLNFV
                     NNFAQLSRNLVGELREELGRERLDQPAIAEILATLEQNSVRIEEHGKRADSIVKSMLQ
                     HSRGRSDERQLTDINALLSEDVNLAYHGMRAQDAGFNVTIETDLDPAVGKLNVIPQDV
                     SRVFLNIITNSFYEVHRKKLEQNGEFKPTLTVKTRSLGGQIEIRIRDNGDGIPPAVRE
                     KLFTPFFTTKPPGKGTGLGLSISYDLIVHQHSGQLLFESEEGRFAEFTIRLPR"
     gene            21581..22294
                     /locus_tag="PKM23_01350"
     CDS             21581..22294
                     /locus_tag="PKM23_01350"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNY90145.1"
                     /translation="MKNLFLFTLLLLCCTPAGHFKPRQILVITGGHDFEREPFFRMFD
                     EFGDITWKEKMHPLSQGAFSDGEQPFDAVLFYDMPDSVTAAVQEEYTRLLQRGTGVVF
                     LHHALCANPGWPEYERMVGGKYLPAAEERAGRRMPASTYREDVEVRVEIADPGHPVTR
                     GLSDFVLHDEVYGGFITRPDIYPLLRTGHPESSPVIGWAHTYDHSRIVYLQPGHDHRA
                     FDDPRFRRLVRNAIEWVSE"
     gene            complement(22302..22526)
                     /locus_tag="PKM23_01355"
     CDS             complement(22302..22526)
                     /locus_tag="PKM23_01355"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY90146.1"
                     /translation="MTLKKFNSLTFQPASRELLGEAVATLHQGILQAGKHRLIWAGND
                     EAGRQAPAGVYIIRMTAPGFSASRKVLLLR"
     gene            complement(22584..23033)
                     /locus_tag="PKM23_01360"
     CDS             complement(22584..23033)
                     /locus_tag="PKM23_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNY90147.1"
                     /translation="MIIDRLENAEAYVKLHPAFARAFAFLRRPDLTELALEKHPIDGG
                     RLFAILSKAPGKVKTEALLEAHRSYIDIQYVIAGCDEMGYKPIITCEHLRTPYDDGMD
                     ILFFADPPESWIKFAAGSFAIFFPQDAHAPMAGTGEIHKAVVKVAVE"
     gene            complement(23268..24128)
                     /locus_tag="PKM23_01365"
     CDS             complement(23268..24128)
                     /locus_tag="PKM23_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNY90148.1"
                     /translation="MLDKILSARLALSAPANAIEQENVLQEILQQYILAGLSRAGFFR
                     EAVFHGGTCLRILHGMNRFSEDLDFLLKQPDLAFRWQPYLEFLQKDCAHEGILLEVQD
                     KSRAGIAVQKAFLKNDSIGKILLLELPFERHRAQKIRIKLEIDCRPPAGSAFLTQFIT
                     FPVTLPVTTQTLESGFALKLHALLSRPYVKGRDWYDFLWYSARGIQPDLALLQNALQQ
                     QGPWADQSIQVTLSWLYDRMAAVIAALDWRAAQNDVQRFLPTSEQEGLRVWGIDLFIQ
                     QLGWTCHINK"
     gene            complement(24118..24777)
                     /locus_tag="PKM23_01370"
     CDS             complement(24118..24777)
                     /locus_tag="PKM23_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90149.1"
                     /translation="MQTLTEHIYHLAPPGGLFDETAIANLFPESTTGARALLVHRAVQ
                     AGEILRLKPGLYLLDRRYRHSEPHPFQIAALLHYPSHISLESALSWHGLIPEAVYQVS
                     SVTAQRSRTFTTPLGVFTFQRVPARLPRAGVQALQVARDTWAFIASPLRAIADMLYLD
                     KTITWKNNGLSWLEESLRIEEEDLRQIPFTDIDDIVTSLRSLRVRNYLQQLKGALHYA
                     G"
     gene            complement(25012..25536)
                     /locus_tag="PKM23_01375"
     CDS             complement(25012..25536)
                     /locus_tag="PKM23_01375"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNY90150.1"
                     /translation="MIAPKDKMETMARFREGAALLENAVKDLPEAVLDAPPARGGWTI
                     RQIVHHLVDGDDIWKLCIKMAMGLDQPEFALSWYWGMPQQDWAERWAYSQRSLDVSLA
                     LFKANRAHILQLVESVPEAWERLALLRNPEGRVEEVPVGSVIQMQGDHVLHHIKRINE
                     ILQEQSDLVKSTRR"
     gene            complement(25574..>27160)
                     /locus_tag="PKM23_01380"
     CDS             complement(25574..>27160)
                     /locus_tag="PKM23_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HNY90151.1"
                     /translation="SDPSLPHLVQENGRHALIVDGEPFLILGAQCHNSSAWPATLPQV
                     WSAMAALEVNTLEIPIYWEQFEPQPGQYDYSVLDTIITQARGHGLRLVLLWFATWKNG
                     SNHYMPQWMKLQPELYPNITGAKGQAVDSPSPHSQAALEADTRAFTAFMRHLKTFDAQ
                     HTVIMVQVENEPGSWDTIRDYSPAAQKLFNEPVPAPALAAMKKSAQAGRSWAEVFGRD
                     ADEFFQVWHVATYIGQVAAAGKAVYPLPLYVNGSIRDPFNPGWPPTYQVGGPNDNVFE
                     LWKAAAPAIDLLAPDIYITDTSAYLKVLELYSRPDNPLFIPETIGHGPLTRFFFAALG
                     RGAIGYAPFGMDDTAIQRGRDGRPLVADEKYGDTALNYRLLEPMAREIARLNHAGKVQ
                     TAVQLAVDSTSGPGSTFDRVHYLTDQTLHFEGWDADIAFGRFNRLARITRQPEGPDGR
                     ILIAELGKGEFLLAGYHCRVLFRPTAEKKERPWQYLQVEEGRYVDGEFRASRILNGDQ
                     TDWGLVFATPTVLRVQLYTR"
BASE COUNT         5719 a         8339 c         7607 g         5495 t
ORIGIN      
        1 ggcgggcctg ttgtaaaacc gcatcgggat ggatgtattt cggcggcctc aatggcttga
       61 tccgctttca ccctgattcg atccgcgttg aaaatgtgcc cccgccggtc gttctgcgtt
      121 ctttccggat cttcaatcgc gagcgcctgc tgcctcgtcg caggagccgc gacgccgcaa
      181 ttgtgctgcg gcacgatgaa aatttcttct cttttgaata caccgccctc gacatcgccc
      241 cgccccacaa acaccgctac gcctacagac tcgagggaat cgatccggat tgggttcagg
      301 ccggagcagg ccagaccgcc tcctataccg atatcgatcc cggctcctac tatttctccg
      361 ttaaagcctg caatgccgac ggcatctgga gcgcgccggt caccctgccg gtccgcatcc
      421 tccccgcctg gtggatgacc tggtggttca aggcggccat cgccctcgcc cttgctgccg
      481 gattctggct gctttaccgc agccgggtca accatctgct ggagattcat cgcatgcgtt
      541 ttgatatcgc cggcgatctc cacgatgaga tcggcagcaa cctcagcagc atcagcgtcg
      601 aaactcaact cctgctcgcc tcccctgatc ttggaaagga gcagcgtgaa cagctcagcg
      661 atatcggtga aaccacccgg gagaccatgg aggccatccg ggatatcgtc tggttcatca
      721 acccccgcaa tgatctgatg gaggatatcc agctcaagct cagggcaacg gccggacgtc
      781 tgcttgccgg tctggaatgg agccttgaac tctccccgca gcttcatttt gaggagctcg
      841 atctggaggt gcgccgcaat ctcttcctga tcttcaagga ggccctcacc aatattgtcc
      901 gccattccgg ggcccgcacc tgcaggatca ggctcttcga cagcagcgcc gggtacgaat
      961 tggagatcca ggatgatggc tgtggattcg aggctgaacc cgctcccgga gagagtggac
     1021 ttctcaacat gcgccgccgc gccgcgcgga tcaaatcgga gctccaggtc ctcagcatcc
     1081 ctggacaagg cacactgatc aggctcatcg tcccgcaccg caggagatga accgcctcca
     1141 ttatccgcta tcgctgtaat tgcaaaaaat accacgatca tgcgattgca ttatgatccc
     1201 gggtgtgcta ttttcagctc aagatgcatt ctccgttcaa gcgcaggctg gcatgtccgg
     1261 cgggcgatcg tttgcctttg gatgccggct gggggcaatc tgaaatggac aggaaaaggc
     1321 catgacgcta cttgggtttg ccaaaacatg ctgcaggcgg aattccttcc gccgcgttct
     1381 cctgcttctc atgctcctgg ccgcagggag tacgctccag gccggcacga ccggcaaaat
     1441 cagcggcaag gtgacagacc gcaagtcgcg cgagccgctg ctcggggtca atattatcct
     1501 ggaggggacg gcgcgagggg cggtgaccga cgcagatggc ttttaccaga tcatcaatat
     1561 tccccccggc agttacaccc tctccgcgcg cttcatcggt tacggctccc agcgcctcac
     1621 caacgtcact gtcagcgtcg atcgcaccac ccagcagaat ctcgctctgg agcagcagat
     1681 gatcgagggc caggaggtga ccatccaggc cgagcgcccc gccatcgaga tggatcgcac
     1741 ccacagcgct gcggtggtca ccaaccggac tgtggagatg atgccggtaa ccaccgtcaa
     1801 tgaagtgatc gagctccagt ccggcgtcgt cagcgccgat ggggaactcc atttccgcgg
     1861 cggccgcagc cgcgaggtct cctatctcat cgacggcgtg ccggtgacca atgcctacga
     1921 ccagagcggc ggcaacaacg tcgtcgtcga aaacgccatg atccaggagc tggaggtcat
     1981 cagcggcact ttcaacgccg agtacggctc ggcccagtcc ggcatcgtta acatcatcac
     2041 caaggggcca gcgcaaaaat tcagcggcag cgcccagatc tatgccggcg actggctgag
     2101 tggtcgcgat gatatctttc tcggcatcaa tcattttaac cccctcagcg aaaaggacgg
     2161 ccagttcagc ctttctggtc cgctctcctc caaaatcggc ctcttcctca ccggacgcca
     2221 caaccgctgg cagagccgcg actggtacca gcgccgtttt aattccatcg acggctggcg
     2281 aatcgccgcc taccggcgct ggttccagga gcacaacctc gccgagagcc agacctccat
     2341 cgccattgcc atcccggaat ccctggcgac cggcgacctc agcgagggac cgttgaccac
     2401 cggcagcagt acctcgcttt cggccaagat cggcttttac cccaccgcca aactgaattt
     2461 cacctaccag atcttcggct cgctgcagca gagcgacggg attgatgcag gcgtgacggc
     2521 cgtggcaggc agtggttcgc gccgctatgc ccctgacggc ggcggcacct ccaaaagctg
     2581 gtcgcattcc catttcatcg ctttgcacca ttttccggcg aacaactttt tctacaatct
     2641 gaacttttca taccaataca atatcggtga ggactattat cgcaaggaca accgcatcgc
     2701 ccgctacccc ggcgatgagg gcatccagtt gctctccgca accgcccagg gcttttcatt
     2761 gggcactacg gacggttttt acaccggcca atcggacaag aactaccgca cccagatgct
     2821 cttcagcggg gatttcaact ggcaggtgga caaatacaat tttctcaagg ccggctttat
     2881 cgccagacga cacaatatca acacctactc ctggggcttc aaggcgaccg atcaatggga
     2941 aaacaacgcc tggccgaacc gcggcctcat cgacggcgcc gattacacct acgaaggcta
     3001 ctgggagcag ttggtcgaat actggaaaaa ctgggagagc atcaaccagt ccgagcggta
     3061 cgtagcctat gccgattccg agtacaccct ctggcgtgat tataacatca aaccgacgga
     3121 ggcggccctc tacctccagg acaagatcga gctcggagaa attataatca atgccggcct
     3181 ccgtttcgac atctttgacc ccaacgagaa gtatattatc aatccccgca cggaggccct
     3241 taacctcggc cggcccgaga acatgaagaa agccgccctc aagttccaga ccagtccccg
     3301 cctcggcatc tcctttccaa tttcggccag cggggctttc cacgccgcat acggccattt
     3361 cttccaaatg cccagcttcg agtacatgta caacagtccc ctctacgcca tgaccaagct
     3421 gcagctggaa ggcaaaaggc tgggcaacgc cgacctcaag gcggaaaaga cggttgccta
     3481 cgaaatcggc ctgcagcagc agctgacccc ggcgattgtg gcggacatca ccgcctacta
     3541 caaggatatc cgcaacctcc tcggcatcga gcagatcacc accctcgacg ccgtctccta
     3601 tacccgcttc atcaaccgcg actatggcta taccaagggt atcaccctgg gtatttccaa
     3661 aaccggcggg gggtgggtga ccggcgggat caactacacc tacgcctatg ccaacggcag
     3721 ttcctcggat ccgaccgcca tctatctgat ccagaccgca acgcagatcg gcggcgcgcc
     3781 ggtgcagttc gccgaccgca agatcctcgc cctcaactgg gaccagcgcc acactctcaa
     3841 cctctacgtc aacctctatc gcccggagaa ctggagcgtc ggcctggccg gttttttcaa
     3901 cagcggcatg ccctacagcc cggtctttgt cgaaaagtac gacatcaacg aacgcgaata
     3961 ccgcaacgcg gagatgaaac catcccgatg gagtgtggat ctaaaggcca aaaaatatct
     4021 gcggctggcg ggtttgcgct cgacgttatt cctcaaaatc gacaacctct tcgatcaact
     4081 caaccataac tcggtgcacg cttccaccgg gcgagccgac caggtggcgc gtctgcctga
     4141 aaaccagaag atcctgctcg atacgctcga gcaggaggga ttgttcacca ttgcagagta
     4201 tgatgtctat ccgggatatt tttccgatcc gcgcaggatc cagatgggtc tggagatcaa
     4261 tttttagccg gagcggcagg cattaccatg tcataaacgg acgggaacca tgaaaagaat
     4321 ccatccatcg ctgcttctga tccgcttcgt catgctgctg ggattgggac tctctgtgac
     4381 cggagccgcc tcggccgggg agattatcag gccatccggc aagcagcccg gaggcctctc
     4441 ctcctggacc aacacccgcg aggcccgaac cctcgacaaa gccgcagcct cccagggcaa
     4501 ctcgcggtat ctgcgctggg ccgtccatga tggcaatctg gtcaccggcc cgaccatcaa
     4561 ctccggcttg ctcgcctacc attatgtcgg cgccgccccc ctgatggcct ggccaaaggg
     4621 cgcccgggct gtgcgctaca ttcacggcgg ggtcttttac tgcgccgccg aagtcaagga
     4681 tgccctcagc aataccatcc acattgtctc cgacaattac cgccgcagca gcgccgagtc
     4741 ctccgccgac ctctcccatt ggtacgcctt tatgcccctc ggacgctatt tcaacctcga
     4801 tcagccgggt tcgcttgaaa ccccggaggt ctacggcatc agtgaggata ccggcctcga
     4861 tggggtgccc tacaccggcg attatggaga agacgacggg atcctgcaga aagatgagga
     4921 ttataaccgc aatggcgagc tcgatgtcag cctgcagaat actgtgggct ggttcgccat
     4981 cagccaccgc aaggagacct ggccccggga ctggccggtc ggctccttcg tcgatgataa
     5041 ccgcagggag ggggagaccc gtcccagttt gcgcgccggg cgctggaacg gcgctttcgg
     5101 cccctatatc cgcgctaccc aggagtccta ttatgtgatg gacgaccgcg aaaacgacga
     5161 attctcctac tatccgttcg acgacaagcg cccctggccc gatggccggc gcggcctcgg
     5221 tttcaccgtc accgtacgcg actaccaatg ggttgcccgg ctctccgaag atatcatgat
     5281 ctccacttac gacatcgtca ataacggcaa ggatctcgaa aaatgcgtcg tcggcatgta
     5341 tattgacccg gatatgggcg gttccctcag cggggatgat gcggcctttg accggcagga
     5401 ggatatcacc tacgcctgga gcgccagcat gctctccgaa gagggctggc cgatgggcta
     5461 ctttggcttt gcctttcttg agagccccgg acttcccttt gacggggtgg acaacgatga
     5521 ggacggcatg acggacgagg cccaaaacaa cgatatcgac gatgacggcg actggcgcag
     5581 ctttgaagac cgcaacagca atggcgtctg ggatagcgag gacctcaacc gcaacggact
     5641 gctcgatgag aatgaggatg tcaacggcaa cggccgcctc gattacgagc ccctcaacga
     5701 cgataccggc agcgacggac tctccccgga atccgacgaa tatattggac ctgatcccaa
     5761 tggcacggag ggcaacggcc gccccgacgg cggcgagccc aactttgagt tcaccgataa
     5821 ctccgagtcc gatcaggtgg gtttgacctc cttctacctg cgcgacgtcg atgacaccat
     5881 ggccgacgat gaaaaatact gggtcaccga gatcgtcccc aacacctttg cggtcaggcc
     5941 cggctaccag cgcgatatcg cctggagcta tggcagcggc tatgtcgcct tcaccagcga
     6001 ggccaactcc caccactacg ccatcgccct tctctttggc aatgacgagg ctgatatcct
     6061 gcgcaacaaa cgcaccatgc aggtgattta tgacaacgat tataatttcg ccaagccgcc
     6121 ccggactccc ctgctcacag ccatcggcgg tgacaaaaaa gtatacctga gctgggacga
     6181 tgccgcagaa cactcgcgcg atcccatcta tgggctcgat ttcgaagcct attatatcta
     6241 tcgcagcacg gacgcctcgt tcggtgaaat caagaccatc accgacggtt ttggcaatcc
     6301 cctgctcttc aagcccgtgg ccatttatga cctcaaggac ggcctcaagg ggattcatcc
     6361 catccgcctc ggcagcgaac tgggtgcaga cagcgatctc ggggtctcct acaacatggg
     6421 cagcgacagt ggcctgcgcc acaattacac cgacactacc gtgaccaacg gccgcaccta
     6481 ctactatgcc gtggtctcca tcgacaaagg ataccatcct tcattttacc cgtcgatctc
     6541 cgaccgcgag gggctgctgc cgatctcgcc aacggagtgc tcggcgacga tccagactga
     6601 tcccctggga cgggccatct gggcggaccg gaacacagcc atcgtcatcc cgcgcgaacg
     6661 cccggccgga tggcagcagc cgaagatcgg cggcgagggc gtgcgccatg tgcagggcga
     6721 tggaacggga ttggttgcga tccgcatcgt caatcctctg gcggtgcggg ataaccacac
     6781 ctacagcctt cagtttcgcg atgacggcgc tttttttgaa ctggacagca gtttcaccgg
     6841 tctgacccga aggatcgctc tttacagcgt caatggcggc aacagcctcg ccctctactc
     6901 tgtcgatgac cccaatacca gcgaggcgat ggcggatttc atctatgacg gctttcaggt
     6961 tctgctgaca aaccatgatg tctccattga caccacctac tgggcgtccg gcacctcagc
     7021 cctggcgctc atcgatatga cccagaccct gagcgggatc gctctgccgc gcgattacga
     7081 gatccgcatt atggaacttg gcgcctacaa accagtcaat atcgccacta ccaccaattt
     7141 tcaggtctgg gatgtaaccg atcccgaggc gccctttcag gttgaatacc gcttcactga
     7201 aagtaaaagc tcatcggtgg cggatcgcgg gttgctcaag tccggctgcc gggtcattct
     7261 tgtcaacaac gccgtcgaga ggcggcagac ctggaaatgg gatttcggct atccggccga
     7321 atccgacagc gccgcctgga gtatgccggt caagggcgac ctcttcaagg tcctcacccg
     7381 caagcccttt gaccgctatg accgtttcga attcaccatg ctcggcaaca ccgtcagcaa
     7441 ccgcaagatc gccgctgacc ttgaaaagat ctacacggtt ccggatccct acatcgccgc
     7501 cagcactctc gagcgcaagg tgatcaacca ggaagagggg aggggggacc gccgcatcga
     7561 tttcgtcaat ctgcccccgg aatgccaaat ctccatcttt acctcctcag gccgactggt
     7621 gcgcgagttg aagcacagcg gcgatgccac catgtcgcgg gagagctggg acctgcgcac
     7681 ccgggatggc ctggagatca cccatggtgt ctatttctac gtggtcgagg ccccaggcat
     7741 cggagtcaag cgcggcaaac tggcggtgat caagtagtga tgtgctgcgg cgggcacctg
     7801 cagctcgcat ccgccgccag catttcaggg atatctaatc ggacaggaat gcctatgaga
     7861 tatgcaatca gtatcgggtt gctgctgctg gccctcgcca ccaccggcaa tgcacagttc
     7921 gaaaccgatg tctccggagt cggcaccagc gccggcacct ttctcgagat cggggtcggc
     7981 gcccgcgccc aggccatggg cggtgcctat gccgcccttt ccaacgatcc gagcgggctc
     8041 tattacaacc ccgccggcat cgtctgggtc aaaaatgccc agctcgaggt gatgcacaac
     8101 gaatggctgg tcggcacgcg ctacgatttt gtcggcctgc tgctgccgct gccctttctc
     8161 aactccacac tcggtacgaa cctgatcatg ctcgattatg gcgaacagcc ggtgcgtacg
     8221 gtcgaacgcc ccgaggggac cggggaactc tgggatgcgc gcgattatac cgttggcctc
     8281 acctgggccc tcgccctgac tgaccgtttt tcctttggct tgacgggcaa atatgtcagc
     8341 cagaagatct ggcatgagac cggcagcgcc atggccatcg atctcggcat ccactataat
     8401 accatgctca agggactgaa gctggggctg gcgatgtgca atttcggcaa cgagatcggc
     8461 ctgcgtggcc gggatctcga tacctcggtg gacccggacg agaaaaatga gaacatcgac
     8521 cgcgtcccgg ctgaatacaa gaccggcacc tatcccctgc ccctgctctt ccgcttcggc
     8581 atcgcctacg agcgggaact gggccggttc ggcaccgccc tggttaccgt ggatgtgaat
     8641 catcccagca attccaccga gagcgtcaac ctcggcgccg aatatggcgt cgccggcctc
     8701 ttttacctcc gcggcggtta tgccaatctg ttcgaaaagg atagaagcaa cggcctgacc
     8761 cttggcggcg gactggatta ttatcgccgc ggcagcatgg gcttccgcat cgattacgcc
     8821 tggtcggatt ggggcatcct ggaggagagc caccgcttct ccgtcggcat actcttcccc
     8881 tgacccgccg gcggagtagc aaagatggta ttgccgccct gaatggatcc atgacaaaag
     8941 gagcaacccc tatgaagaag accctttgca tcctgattct gctcaccgcc ggaatggccc
     9001 tggcgcagat caatctgccg gccgaaggac tcgtcggcca ttggaccttt gacaatcccg
     9061 caaatctggc ccaggccacg gcaggcaacg acctggtccc cgacaagctg accggcaccc
     9121 tcaatttcaa ggcggtgccg ggtccggcca ccggcaatgg cgccgtcaat gtcggcatcg
     9181 gcagttttta caggtgctac cacgacatcg aggccaatgg ccatgatcca gcactgcccg
     9241 actccctgcc cacgcgcgtc aaccgcttcg ccgtcgtcgt tgacgttcgc ctccctgcaa
     9301 aagaggtctg gtataccctt ttcgccaccg acaacaacgg ggacctggcc gagagcgacg
     9361 gggacttttt tatccgcacc gacggtgctc tcggcctcca ggccaccggc tactccttct
     9421 acaagatcca ggacaccgaa cactttttcc gcctggtcat caatgccgac ctcggcaatt
     9481 attataatta ttatcttgat gggcagctgc tgcgtgaggg cggcacgcgc cgccttgacg
     9541 accgcttttc ccttgacagc cccgatggct ccaatcaggt gcttttcttc ggtgacaatg
     9601 acggcgagga ttctcccatc gacatcgccg agctcgccct ctacaaccgt cccctgacgc
     9661 cggccgagat cgccgcgatg ggcggctatg gccatttcat cgaatcggag acgccggtct
     9721 ccatctggaa ctttgacaaa cccgacagcc tcttctgggc cagggtgggg gagccgctcg
     9781 agctgatggg cggggtcaag gccgctccgg gcccgaacag cagtgacggc gcggccgcgg
     9841 tcggtggcgg ggcctggctg aaggcccttg tcaacatccc ccccaacggc cgggacaacc
     9901 cgcagaacgt caaccgctat accctcgcca tggacatccg ccagccgcag cgcggcaaat
     9961 tcagcgccct gctgcagacc aatccggaca acaacgatga cgccgacctc tttgtcaacc
    10021 ccgacgggcg catcggctcc tccgccatcg gctggagcga ttcaaccctg tacgtcgggg
    10081 agtggtcgcg cgtggtcatg acctgtaacg ccggggatac aacatccagc ctcatcctct
    10141 atgtcgatgc caaatcctgc ctcaaggtcg cggagctggc cactgacggg gatctttcgc
    10201 ttgcggcccg gaccggcctc aaccgcatgc tcttttttgc ggatgaaaac aacgaggacg
    10261 ccccgctaga ggtggccaat attacccttt ataaccggcc gctcagcgaa aaggaggtga
    10321 tcgccctcgg aaaatatgaa cacctgcgca gcaaagaggt caccccggcc ctgcacactg
    10381 tcaaattccg catcgccgac gagatccagt atgccgcgat tccccatcat cccgattttg
    10441 atttcggcgc cggcaccagc ttcacgatcg aatgctggat gcgcgcagat cttccctatg
    10501 agggcgatcc ggccttgatc tccaacaagg actgggacag cggcgccaat ccgggttggg
    10561 tgctctccgc gcgcggtgat gactggaagt tcaacattgc tgacaaaacg ggcctgcgct
    10621 tcgacgccgc catcaccact cccgatgtta atgacggttt ctggcaccac ctggcggtca
    10681 ttgtcgaccg cagcaaacag gagtatgtgc tcatcaccga caacagcgtc accgtcgccc
    10741 agagcatggt ctccggggag ggcgtcgccc tgggcgatat tgacaccggc ctgccgatag
    10801 gcatcatgca ggacggcacc ggcaactatg cctggggcta caaaatgcct ggagcggtcg
    10861 atgaggtgcg catctggaat tccgccctga gcccggagac ggtgaaaaaa tgggcccacc
    10921 gcctggtcac cgccgaacat cccgattacg ccagcctgat cacctacctg aagttcgacg
    10981 atgcctcggg tcgggtggtg actgacgcca gcggccgcgg ccatcatgcc gaactcaagg
    11041 gccagatcga atggcaggtc tcctacgccc ccctggccga tgccgtcgcc gacggtctca
    11101 aggatctgtc cgggatctgg ggtggccaga aagccggcgc ctcgggaggg atgaccctga
    11161 ccgccgattt caaattcaat ctggccaagc ttcctgctgc ggcggcatcc ggaccggtcc
    11221 aggaagcaga tatcgaatct tacctcaatg aacaatacgc cctcagcggc cacaacagcc
    11281 tctccggcgt ggtcaagacc gaacttccgg cgatcgtcct ggcacgttac ggccgcgtct
    11341 ggtatctgga cgcctcggct tcttataccc agaagaccaa agtcctcttc accttcaatg
    11401 aaaccccggg cgcggctgca ggctatgttc tgctcaaccg cgccggcgca tcgggcaatt
    11461 ttgcagcgct gggtactgca gctgccgtcg atggcaataa tgtcatcttt gccaacctga
    11521 cgctgaagga tggcagctat tacaccctgg gcacgaaaga cgagaccgct tcgccgctgg
    11581 gcaccctcag cggcatcacc ggggaaggcg gatcaaattt cacctatgaa ttgaagaatg
    11641 cctggcccaa tcctttcaat ccgagaacga ccattgccta ctccctggcc aaaagctcgc
    11701 aggtgcggct gacagtgtac aatcagctcg gccaggcggt cgccaacctg gtagatggca
    11761 agcaggaatc agccggccgc tatcaggtcg tctgggatgc ccgcagcatg cccagcggac
    11821 tctatttctt ccgcctcgag gccggggctt tcaccaagac cgggaaaatg gtgctcgtca
    11881 agtgagccgc catggcgaca aacccttctc ccgctgctgt actgcagcgg gagaagatca
    11941 gtattacaga gcagcccata tggcgctcct tttcatcgcg atctttttcc tcgccgccgg
    12001 ctgcggcagg aaaaagcagc cccgggatcc agccgtcgct cgagtcggag accgggtcat
    12061 cacggtatcc gaattccgca gcaattatga atttggcatg cccttcctga aaaaaggccc
    12121 cgatccaaag tccgcctatt tggactatat gatccgggag aagctccttg ccctggaggg
    12181 atatcggctc ggctttgacc gccaccctcg tgtccgtgaa aatgaagcag ccttgatgcc
    12241 cgagctcctc atcgaggagc ttttcgacca cgagatccgg cgtaaaatca cggtcacgac
    12301 cgaagagatc catgaaggaa ttaccaaagc gaaggtgagc tggaaattcc gttactggtt
    12361 cgagcccaac cgggccgatg ccctggctgt ccggacggcg atggaggagg ccggctacgc
    12421 ccgcgtggtc gcggagctgc tcgaaagcaa tcccgagatc aggctggagc ccgcagattt
    12481 tgaaagcggc tacctcacct ggctggagat tgatccggcg atcctcgcgg cgatcaagga
    12541 tttacccgtc ggggagatct cggagccgct cttcatcaac ggtgcctggg ttcttttcca
    12601 gattactgat atccggcggg aagcgttcac cgagaatgcc ttccgcgaag gggccgagag
    12661 catgcggcag gtcctgttct accgcaaggc acagacggca gtgcggcgtt atgtcgacag
    12721 tctgatgacc ccgctggatg tgcgcaccaa ggcggaatcc tttcgtttac ttgccgatgc
    12781 gctggcggaa tggacggccc ttgaaccggc ggaaaggggg gaggtgctgg ccgtcgccgc
    12841 caaagccggg ccggaacagc cccacctccg aaaactgaac aaccatcttg acgctccttt
    12901 gacggaattc gccggcggcc gctggaccat cagcgatttt ctgcagcgat tcgatcccgc
    12961 atctatcaag gcgccggcag gcgatctcca cgcctttcgc aagtccatca accagcgttt
    13021 ggctctcacc ctccgggacc actatttggc cggagaagcg cgcaagctca agctgcataa
    13081 aagccctcga ctccagcaca gcttgcagca gtggcgagac aaatgggtct atgatgaggc
    13141 gcgcagccat tttctcagga gcgtaatggt ggacagcgcc gccacccgga cctttttcca
    13201 gcaaaacctc gctcgctacc ggctgcacaa gcgtaaagaa cccctcttca ccgaatttag
    13261 tgaggaggcg aagcgggatg cgtggctgca aaaggccagg gaaatgctga acggggaggc
    13321 tgcggcgctg cagcggcgct atcccgtcac catccatcgc gccatcctgg atactattca
    13381 cgtcacggct gctgcagaca ataaatggat gagcgtgcag ctcttcaagt cgggcagcca
    13441 acgcatggct gctccgatcg cggatccggc atggggcctg tagctgatcg agccggccgg
    13501 gagcgatgaa ttacgagtgc gcacttgctc aggaatgggc caagatatga aatcagatag
    13561 aggcagcacc cccgcgggtc cgatcgacct gtggctgatc gaggacaatc cgcgctaccg
    13621 caagaccatc gccggtctga tggagcacac cggagggatc cactgctcgc aagccatggc
    13681 gacctgcgag gaggcaatcc ggttggccga aagcgaatct cctccccagg tcattctgct
    13741 cgatatcggc ctgcccggca tgaacggcat tcagggcatt gaggtcttca agtccctgct
    13801 cccgacgaca catatcataa tcctcaccat ctacgatgac aataacaaag tgctggaagc
    13861 gatctgcgcc ggggcttcgg gttatctgct caaggattcc cctcccgaaa aaatcatcga
    13921 atcgatccag gaggttatgg ctggcggtgc ccccatgaac atgcagatcg ctgccaaggt
    13981 gctcgagatg ttcagagagc actatcccaa aaaatccgat tacggcctga ccgagcgcga
    14041 gaaggaaatt ctgcagcacc tggtctccgg cctcaccaaa cagcagatcg ccggcaagct
    14101 ctttctcagc tttcatactg tgaacaccca tatcaagaat atctacacca aactgcacgt
    14161 caatacgcgc tcgggagccg tctccaaggt ttacaaagag aaactgcttt gagcagcctg
    14221 cagctcctgg accaggctgg atggcagttc cgcaccagtc agggcacaaa ataccacgat
    14281 catgcgattg ttttattgcg cctgtacccg tagttttaac ttcaggcgaa gcggcatgat
    14341 cgcgacaatt cccccaaagc ttgctgcatt tgaaattcgt gaaggaggta gccccatgaa
    14401 aaatagttac ctgtatcggt tgacgctcct tttcgcgctt ttttcggcgc tggcggtatt
    14461 cgctcaggct ccggatcctg cggggtggtg gaaatttgac gatccggcgg acctcctcaa
    14521 gccggaggct ggcttcggcc ttccccttga gctggtgggc acgcaccaga gcgtcgatgg
    14581 cccggcggcc ggcaacggcg cggtcaaaat cgggatcggc agtcactaca aaatgatgca
    14641 tggcatcgcc cccaacggtg gtggcagttt tgtcaacgag ttcaccctga tgttcgattt
    14701 tcgtgtcgag cagctgaaca agtggtatac ccttttccag accgcgatgg agaacaacaa
    14761 cgatggcgac tgcttcatcg acaaggaggg caacattggc accggctcga ccggatacac
    14821 cgactatgcg gtcatcccca atgaatggta tcgcctggtt atctcggtga aaaatggcac
    14881 gcactatcgc tactatctcg atggtctgct ggcattcgac gccgacatcc aggatatcga
    14941 tggccgctac ggcctcgagc cgctgctact gctcatggcc gatgacgacg gcgatgacgg
    15001 ctccatcgaa attgccgagg tcggtatctg gagcacagcc ctgacctcct ggcaggtgca
    15061 gtcgctcggt ggctacgacc accagcagcc gctcgatccc atgcagccgg cgggaagatg
    15121 gaaatttgat aacgtcgaca atctggctga gggatatttc ggcaaggatc tggtgctcac
    15181 cggcagcgaa ctggcggtcg aaggcccctc cacgcagaac aaggctgtgc gcatcggacc
    15241 gggcagctat tatatcatgg agcccggcat cgcacccaac ggcggcggct cctacatcaa
    15301 tgagttcagc atgcagttcg atttcaaggt ccccgaaatc ggcaaatggc acgcttttct
    15361 gcagaccaac accagcaacg gcaatgatgg ggatcttttc gtcaatgccg acggccagat
    15421 cggcgtcgcc tccaccggtt acagcgacct caagatcaat gccggggagt ggtaccgcct
    15481 gatcatctcg gtccggaatg gcgagttctt ccactacttt ctggacggca aactcctttt
    15541 tgagggtctc aagcaggatc tggatggccg tttcggtttg gaggacaagg tgctgctctt
    15601 cgcggacaac gatggcgagg atggcgagat cgacatcgct gatgtcgcct tttgggggag
    15661 ggcgctggcc gacaccgaag tggtcaaact cggcggctat gcccatgaga gcaaaaaggt
    15721 tgcaggcagc tggaaattcg acgatgccgg cgacatcctc aaggccgaag ccggcttggg
    15781 cctgcccctg gagctggtcg gcagccatga agtcatcgaa ggacccgcgc ccggcaatgg
    15841 cgcgatccgg atcgacaagg gcagccacta caagctgacc catggcatcg ctcccaacgt
    15901 cggcggactc ctcgtcaatg aatacacgat ccatgcggat atcaggatcc ctgttctctc
    15961 cggctggcac accctctatc agaccacgcc ggcgaacaac aatgacgggg actgtttcat
    16021 taaatcctcc ggtgtaatcg gcaccggtga caccggctat tccaccgatg ccatcctcgc
    16081 caacgaatgg taccgggtgg tcatctcggt caaaaacggc acccactacc agtactattg
    16141 ggatggccag ctggtttacg aggcgacggt ccaggggatt gacagccgtt ttgctcttga
    16201 accggtgctg ctcatgttcg gagacgatga cggcgacgac ggcagtatcg actgcgcaga
    16261 gatcacggtt tggaattacg ctctcaatgc ggatgaagtc gctgctctgg gtggattcgg
    16321 ccatgtggtc ggtgttgagg gaaacgaggc gggcgccatc gtgacccgct acgagatgga
    16381 ccagaactat cccaatcctt tcaacccgga aacctcgatc tcttatgccc tggtcaagtc
    16441 cggacacgtc cgtctcgagg tctttaacat gctcgggcaa aagatggcca ccttgattga
    16501 tcaccagcag gagagcggcc ggcacagcct ccgtctttcc ggcgcgggct ggccaagcgg
    16561 cgtttacttt tacaagttat cggcgaacgg attcacccag accaaaaaaa tgttcctttg
    16621 caagtgactc aaacggtgca ggctccattc ctatgaagaa aaccattctc ctgcttttac
    16681 tcatcgcagc cgcgacctcc ggccagatcc cgcatccgat aggctggtgg aaatttgatg
    16741 atccggcctc cctcacccgg gcggaggccg gtttcggccg taacctcgag gctgtcggga
    16801 gtgccgttac tgcagtctcc ggccctgaca gcgggaatgg agccgtgcgc atcggtacgg
    16861 gcagctattt caaactggac cacgacatcc ccggcaacgg ggacggaatc tatctcaacg
    16921 aatacaccct tgcctttgat ttcaaggtac ccgccctcgg cgtctggtac tgcttttttc
    16981 agaccaacac agccaacagt aatgacgggg aatgtttcat taatccctat ggcaatgtcg
    17041 gcgtcggcgc taccggttac tccggcgcca gtatcaagcc cggcgagtgg taccggctga
    17101 tcctctctgt gaaaaacggc tccttttttc agttctatct ggacggtgtg ccgttcttca
    17161 gtggagccac gcaaatccgg gatgggcgtt tttccatcga gaatccactg ctccttttcg
    17221 ccgacgagaa cggcgaggat ggagccatcg actgcgccga ggtggccatc tgggatcgcc
    17281 ccctcacggc cgtcgaggtc aagctgctgg gcggatttgg ccataccttc cactatgatt
    17341 taatgacgcg cgtcccctat ctgcaggcgc ccacccccac ctctatccag gtctgctggc
    17401 acgacagctc agccgttcct tcacgggtgg agtacggact ctcccctgca ctgggagaga
    17461 gcgctgccgc cagccaggag cagctgagcg ggccctaccg ctggcatgcc gccaaactcg
    17521 cgggactgca gccggacacc gagtactttt accgggtggt cagcggggat ggcgcctccc
    17581 cgctgtgtac tttccggaca ctgcccgaac cggccaaggc cggcaagctg cgcatgctgc
    17641 tgctcagcga tacccactcc ggcgattcca ccatggtcaa ccgggtcgtg cgtgcagcca
    17701 gggctaaaat cggagcgctt tacggtgagg atctgcataa tcagctcaat ctggtcttcc
    17761 attccgggga tctggtcgtc agcggcggag tgatcaacca gtataccgat cagtatttcc
    17821 gccccatggc gcagctttcg tcctcggtcc cttttatgac cgtcaccggc aaccatgaag
    17881 gggagagccc ctattattat cagtacatga aatacgacga tcactccctg ctcgccccgc
    17941 cgagcgccta tgcagagcgg atgtgggagc tgaaagccgg caacaccctc ttcatcggcc
    18001 ttaacaccaa tatcgtatcg gcggcggggg cgctccaaaa agccctcctc gacggccgcc
    18061 tgcaaaaggc cgagcaggat cccgccatcg atttcgtctt catatttttc catcacctgc
    18121 cctggtctga actgtgggtg gagggagtga ccaatgatgc gggcagcaat tttatacgca
    18181 gggagctgtt tccggtcatc aggaaatata ccaaggtgca gcagcttacc tacggtcata
    18241 cccatgcctt cgagcgcggg acggtcgaat ccccgatgcc tgacggcgat ttccgcatcg
    18301 tctgcgccgg cggcgggggt ggcgccaccg ataactgggg cgaattccag aacattgacc
    18361 atcccttcat ccatgtcgcc tatgatcact attttttcca gatcctcgag atcgatgtac
    18421 ccaaccattc gttcgagatc tcgatgtaca gtcttggcaa tcagtttaaa agtcgggagg
    18481 cccagctcat ggaccgctgg taccgcaagg ttagccagca tggacccgat acgccggtaa
    18541 ttgagactcc tattatgcag gaatcggcgc ttgtgctgaa gagctcaccc ttttcagggc
    18601 ccgattccct catgagcgtg caaatccaga ttgccgaagg ggcggagttc tccagcccga
    18661 aaatcgatac cctggtgcac tggcgcaaca tctacggtgt cgacgccaac tatgaacctg
    18721 tcgacaagaa cgcagggatc gatctgacac ggaccgcttt cagccgggcg cgtttccaga
    18781 ccggcaaatc ctacgcctgc cgtgtgcgct atcgcgatca caatctgaag tggagcggct
    18841 ggtccaatgc cacttctttc aacgtcgaag cttcagtcgg acttggggat gagagtcctg
    18901 ggcgctatga actggcgcag aactacccca atcccttcaa cggcagcacc atcatcgcct
    18961 atcagatccc caatcccgct cacgtttctc tcaaactcta caaccttctc gggcaggatg
    19021 tccgcactct ggttgacacc caaatggccg ccggcatgca ccgcgtcgag ctggatagcc
    19081 gtggccttcc gagcggcctc tatatctacg aactggcctg tgatgccttc ctggtccgga
    19141 aacggctcaa tctggccaaa tagccggccc ggctttccat ccttctgcct ccttaatttg
    19201 gtttgtgatc ggatccggat gtaaaaagag ccctgaaatt tcagggctcc tttatatcgt
    19261 ttctactttt tatggcttgc tttgcgtatc ctcattggtt attttatgga tagaaagaga
    19321 acgaatcaac tcccgaccag atgaggccct gtgtagcccg agtctggtat gcgccgttgc
    19381 attccaaccc gatcttctgg aaggcggaca tgacaacaga ttccggagca aaaccgcatt
    19441 tcgtcaagag atggctgaac aggctgatca gcgccttccg gctctcttcg gaggacaagg
    19501 aactcacccg cacggtcatc gatctggaaa acatccgccg cgtcttctat ctctcgctcc
    19561 tcgccatccc caccagcgcc tgctatttca tcttttttct gatcaagtcg gccggcgaga
    19621 ccggcgtgac ctatcaatgg cggcacgcaa tctggatctg ccatgccatc ctgctggtca
    19681 gctttatcgt catcaacctc gtcatctact tcttcgccta caagcccggc aagaacagcc
    19741 cgctggcgct ggcttgcatc cacatcaccg cagtgatcct tctgctggag ggcgcagccc
    19801 tggctgcggc cgatcagctg gtcaccgaca acatcaccgc ctacctgatc acctgcctca
    19861 tcaccggcct cgttctgctg acgccgccga ggttcgccct gctctattat ctgatctcct
    19921 tcaccgtgtt ttatttcgcc atctccctga cccagaaaaa tcccgcaatc ctgatctcca
    19981 accagatcaa cggcctgacc gcggccgccc tcggcttctg tctttccatc attctgtggc
    20041 gcggctacct catccgggtc agacaaagcc gggtgatcga aaaacagaac agtgagctga
    20101 aaacggcgct cgatcaggtc cattcccaaa aggaggatgt ggagcagctc agccggatcg
    20161 gccgggatat cacctcgtcg ctgtcgatcg agaatatcat ccataccatc tacgagaatg
    20221 tcaacaccct gatggatgcc tctgttttca ccatcgggct gcacaatccc ggcaagggcg
    20281 tactcgaatt ccccgccacc atcgaggaca atcgctccct gccgcccttt tctgtggccc
    20341 tttcggatga aacacatctt gcggtgcgct gtttcaacca tcaccgggag gtcgttatta
    20401 acgactgcag cgctgaatgc ggtgaatatg gcggagctct gacctcctca ctgacggggg
    20461 aaccgcccct ttcatttctc tatctgcccc tgtggaagaa ggacaaggtc atcggtgtga
    20521 tctcggcgca gagccgcgtc agaaatgtct atgatgatta tcatgtcaat atgctgcgta
    20581 acctggcggc cttcagtgcg atcgccctcg acaacgccga agcctaccac cggctctcgg
    20641 ccctgctcga ggagctgagg agcacgcagg aaaaactggt cacccagtcc aagctcgccg
    20701 ccctcggcgc ccttaccgcc ggcatcgccc acgaaatcaa gaatcccctc aatttcgtca
    20761 acaacttcgc ccagctctcc cgcaatctgg tcggggagct tagggaagag ttgggccgcg
    20821 agcggctgga ccagccggcc atcgcggaaa tcctggctac actggagcag aattccgtca
    20881 ggatcgagga gcatggcaag cgggcggaca gcatcgttaa aagcatgctg cagcactccc
    20941 gcggccggtc ggatgagcgc cagctcaccg atatcaacgc cctgttgtcc gaggatgtca
    21001 atctggccta tcatggcatg cgcgcccagg atgccggttt caacgtcact atcgagaccg
    21061 atctggatcc agcggtgggc aaactcaacg tcatacctca ggatgtcagc cgagtttttc
    21121 tcaatattat caccaacagc ttttatgagg tgcatcgcaa gaaactggag caaaacgggg
    21181 agttcaagcc caccctcact gtgaaaacac gcagtctcgg cggccagatc gagattcgca
    21241 tccgcgacaa cggcgacggc ataccaccgg cggtcaggga aaagctcttc accccctttt
    21301 tcaccaccaa accccccgga aagggcaccg gcctcgggct ttccatcagc tatgatctga
    21361 tcgtgcacca gcacagcgga cagctgctct tcgaaagcga ggagggccgg tttgcagaat
    21421 tcaccatccg cctgcccagg taatatacat acgccggagt gagcgggatg atgccattct
    21481 gactgcaggg actaaccggt ttgcagcctg ttcatccacc cgtatctggt ccattttact
    21541 gccaatcccg tttatattgc tatcagtgga gaaaaccccg atgaaaaatc tgttcctctt
    21601 tacgcttctc ctgctatgct gcactcccgc tggtcatttc aagccgcgtc agatcctcgt
    21661 catcaccggc ggccatgatt tcgaacgcga accctttttc agaatgttcg atgaattcgg
    21721 cgacatcacc tggaaggaaa aaatgcaccc gctctcacag ggggcttttt cagacggcga
    21781 gcagcctttt gacgctgtac ttttttacga tatgcccgac tcggttactg ccgcggtgca
    21841 ggaggaatat acccggctgc tgcagcgggg gacgggcgtg gttttcctcc atcacgcgct
    21901 gtgtgccaat ccaggctggc cggagtatga aagaatggtg ggggggaaat atctgccggc
    21961 ggcggaggag cgcgcgggcc ggaggatgcc ggcctccacc tatcgcgagg atgtcgaggt
    22021 cagggtcgag atcgccgatc ccggtcatcc ggtgacccgg gggctcagcg attttgtcct
    22081 ccatgacgag gtctacggcg gattcatcac ccggcccgat atttatccgc tgctgcgcac
    22141 cggtcacccg gagagcagtc cggttatcgg ctgggcgcac acctatgacc actcccgcat
    22201 cgtctatctg cagcctggtc acgatcaccg ggcctttgac gatccccgct tccgcaggct
    22261 ggtgcgcaac gcaatcgagt gggtcagcga atagtccggg ttcagcgcag cagcagcacc
    22321 ttccgcgagg cggagaagcc cggggcggtc attctgatga tatagacccc ggcgggcgcc
    22381 tgcctgcccg cttcgtcatt tcccgcccag atcagccggt gttttcccgc ctgaagaatc
    22441 ccctgatgca gggtagcaac cgcttcgccg agcagttcgc ggctggccgg ttggaaggtc
    22501 aggctgttga acttcttcag ggtcatctcg gctatcaggg ttgcggcacc gctggccggc
    22561 tcgaggttat ctgagcagcc ggtctattca acagcgactt tcactaccgc cttgtggatc
    22621 tcgccggttc cggccatggg ggcatgggcg tcctggggga agaagatggc aaacgaaccc
    22681 gccgcaaact tgatccagct ctccggggga tccgcgaaaa agaggatatc catgccgtca
    22741 tcatagggcg tgcggaggtg ctcgcaggtg atgatgggtt tatatcccat ctcatcgcag
    22801 ccggcgatca catactggat gtcgatatag ctgcgatggg cttcaagaag cgcctctgtt
    22861 ttgaccttgc ccggggcttt ggagaggatg gcaaagagcc ggccgccgtc gatgggatgt
    22921 ttttccagcg ccagttctgt cagatccggt ctgcgcagaa acgcgaacgc ccgggcaaag
    22981 gccgggtgca gttttacata cgcttcggca ttttcaagac gatcgatgat catgggatct
    23041 cctttatttt cagtggaatc cggtgaagcg agggggatag aacggcttcc ggtgagcgcc
    23101 tccggacaga gaggtagtgc atgcgaccga tcgtgctatg ggtggttctt tgatcttctc
    23161 gtccccataa tgttttcctg gcaggggatg ttctccacga tccataatgg gataatgatc
    23221 gaagtggctg cgttagctgt gcacattctt cactctccga tctgccatta tttgtttata
    23281 tggcaggtcc acccgagctg ctggataaaa agatcaattc cccatacacg cagcccttcc
    23341 tgctctgacg ttggtaaaaa acgctgcaca tcgttttggg cggctcgcca atcaagcgcc
    23401 gcaatcactg ccgccattcg atcatacagc caggagagcg tgacctggat ggattggtcc
    23461 gcccaggggc cttgctgctg caatgcattt tgcagcagtg ccagatcagg ctggatgccg
    23521 cgtgcactgt accagagaaa atcgtaccag tccctgcctt taacataggg acggctcaac
    23581 agggcatgca gtttgagcgc aaagcccgat tccagcgttt gggtggttac cggcagggtc
    23641 accgggaagg tgatgaattg ggtcaggaaa gctgagccag ccggcggacg gcaatcgatc
    23701 tccagtttga tgcggatttt ttgcgcccta tgccgctcga atggcaactc cagcagcagg
    23761 attttgccga tggagtcgtt tttgagaaag gctttttgca ccgctattcc ggcgcgtgat
    23821 ttgtcctgca cttccagaag gatgccttca tgggcgcaat ccttttgtaa aaactccaga
    23881 tagggctgcc agcggaatgc gagatcgggc tgtttgagca agaaatcaag gtcctcggag
    23941 aatcggttca tgccatggag gatgcgcagg caggtgccac cgtgaaacac agcctctctg
    24001 aaaaaaccag cgcgggagag tcctgccaga atatactgtt gcaggatctc ctggagcaca
    24061 ttctcctgtt cgatggcgtt agcgggtgca gacagggcca gccgggcgct caagatttta
    24121 tccagcatag tgcagcgctc ccttcagctg ttgaagatag tttcgtacac gcaaactgcg
    24181 cagactcgtt acgatatcgt cgatatccgt gaacgggatc tggcgaagat cctcttcttc
    24241 gatgcgcagc gactcctcca gccaggacaa accgttgttt ttccatgtga ttgttttgtc
    24301 caggtagagc atgtcggcga tcgcccgcag aggcgacgca ataaaggccc aggtatcgcg
    24361 ggcgacctgg agcgcttgaa caccggcgcg cggcaagcgg gcgggcacac gctgaaaggt
    24421 gaaaacgccc agtggcgtcg taaaggtccg gctgcgctgg gcagtcacac tgctgacctg
    24481 gtaaacggct tcagggatca agccatgcca ggagagggct gattcgagac tgatatgcga
    24541 aggataatgg agcagtgcgg cgatctggaa gggatgaggc tcagagtggc ggtaacggcg
    24601 atcgagcaaa tagagtccgg gtttcagccg tagaatctct ccggcctgaa ccgcgcggtg
    24661 gacgagtaaa gcacgagcgc cggtagtgct ttcggggaag agattggcga tcgccgtctc
    24721 atcaaagaga ccgcccggcg gggcgagatg atagatatgt tcagtcagtg tttgcattct
    24781 tgctattata ttacgaatat cgtaatctaa tagcaagaat aatcgtcgat cagatcaaat
    24841 tttgggttat tcgcaaacgg gtgcctgatc aaccagattc gccaggagca ccgagatgta
    24901 atatccccac tttattcgat cagactgctc actacgcact tttcctgcaa agggcgcgcg
    24961 ccctgtcctt ggacgatagc gtttttttcc gttcagcgga gctttgtccg atcatctcct
    25021 tgtggatttg accagatcgc tctgctcctg caagatttca ttgatccgct tgatatgatg
    25081 cagcacgtga tcgccctgca tctggatgac agatccgacc ggaacctctt ccactctacc
    25141 ctccggattc cgcagcaggg cgagacgctc ccatgcctcc gggacacttt cgacaagctg
    25201 gaggatatgc gcccgattgg ctttaaagag ggccagggag acatccaggg atcgctggct
    25261 gtaagcccag cgctccgccc agtcctgctg cggcatgccc caataccagc tgagggcgaa
    25321 ctcgggctga tcgaggccca tggccatctt gatgcacaat ttccagatat catcgccgtc
    25381 gacgaggtga tgcacgattt gccgtatggt ccagccgccc cgggcaggag gtgcatccag
    25441 cacagcctcc ggcagatcct tgacggcatt ctccagcagc gccgcccctt cacgaaagcg
    25501 ggccatcgtt tccatcttgt cttttggtgc gatcatatta tacctcattt tcagataacg
    25561 ctggcaggcc ttgtcagcgc gtatagagct gcacgcgcaa aacggtgggc gtcgcgaaaa
    25621 ccagtcccca gtcggtctga tcgccattga ggatgcggct agccctgaat tcaccatcga
    25681 cgtagcggcc ctcctcgacc tgcagatact gccagggccg ctccttcttc tcggcggtcg
    25741 gccggaagag gacccggcag tgatagccgg ccagcagaaa ctctcctttg cccagctccg
    25801 cgatcaggat gcggccgtcg ggcccctccg gttggcgggt gatacgcgcc agacggttga
    25861 agcggccgaa ggcgatgtcg gcatcccagc cctcaaagtg cagcgtctgg tcggtgagat
    25921 aatggacgcg gtcaaacgtg ctgccgggcc cggaggtgga atcgaccgcc agctgcacgg
    25981 ccgtctgcac cttgccggcg tgattcagcc gcgcgatctc gcgcgccatc ggttcgagca
    26041 ggcgatagtt gagtgcggtg tcgccatact tttcgtcagc cacgagaggc cggccatcgc
    26101 ggcctctctg tattgcggta tcatccatcc cgaagggggc gtagccgatc gcaccgcgcc
    26161 cgagggcggc gaagaagaag cgggtgagcg ggccgtggcc gatggtctcc ggaatgaaga
    26221 ggggattgtc cgggcggctg tagagctcga ggaccttgag gtaggcgctg gtatcggtga
    26281 tgtaaatgtc gggcgcgagc aggtcgatgg ccggggccgc ggccttccag agctcaaaaa
    26341 cgttgtcatt cggaccgccg acctgatagg tgggcggcca gccgggattg aacggatccc
    26401 gaatcgagcc gttgacgtag agcggcaggg gatagaccgc cttgcccgcg gcagcgacct
    26461 ggccgatata ggttgccaca tgccagacct gaaagaactc gtccgcatcg cgtccgaaaa
    26521 cctccgccca gctgcggccg gcctgagcac tctttttcat agcggccagg gccggcgcgg
    26581 ggaccggctc gttgaagagt ttctgcgcgg ccggggagta atcgcggatg gtgtcccagg
    26641 aacccggctc attctcgacc tgcaccatga tgaccgtatg ctgcgcgtcg aaggtcttga
    26701 ggtggcgcat gaaggctgta aaggcccggg tgtcggcttc cagcgcggcc tgactgtgcg
    26761 gggaggggga atccaccgcc tgccctttgg ccccggtgat gttgggatag agctccggct
    26821 gcagcttcat ccactgcggc atatagtgat tcgagccatt cttccaggtg gcaaaccaga
    26881 gcagcaccag ccgcaaacca tggccgcgcg cctgggtgat gatggtatcc aggacgctgt
    26941 aatcgtactg gccgggctgc ggctcgaact gctcccagta gatggggatc tcgagggtat
    27001 tgacctcgag ggccgccatg gcggaccaga cctgcggcag ggtggccggc caggcgctgg
    27061 agttgtggca ctgggcgccg aggatcagaa agggctcgcc atcgacgatc agggcatgac
    27121 gtccgttctc ctggacgaga tgcggcagcg agggatcgga
//
LOCUS       DAOPWU010000010        25516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_1914_length_25516_cov_5.340442, whole genome shotgun sequence.
ACCESSION   DAOPWU010000010 DAOPWU010000000
VERSION     DAOPWU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1914_length_25516_cov_5.340442"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PKM23_01385"
     CDS             <1..1195
                     /locus_tag="PKM23_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90152.1"
                     /translation="LSRIDTTILGYATVQDDRGTPWLVTGGAEGETLAATTLEELRAG
                     LESPGPCTVQFSGEIAGSTVIAIQSDKTLLGVGKAAHLRGIELAINGARNVIIRNVIL
                     SHVPDAGETNDAMSINGGSQNIVIDRCEFFSDRDHGKDYYDGLLDIKNRSSFITVSNC
                     AFHDHWKAILISSGDTQYADSLIRITLHHNHFYNIGSRLPLIRFGKAHIFNNFYQNCD
                     DAINTRMGACVRVERNYFENVGKGLFSDYSAAEGFIQLVDNHFGGASYITAPLCSFDP
                     PYAYADRMHKVELLPALVPARAGTLVPADKVVAAPGRLSLSQNYPNPFNPVTLLRYYV
                     PGSSSVTITVYDRLGKEVATLVDGLRSGENRVEFRGDELPTGIYFITLRAGVQVVTRK
                     ILLLR"
     gene            1270..2862
                     /locus_tag="PKM23_01390"
     CDS             1270..2862
                     /locus_tag="PKM23_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90153.1"
                     /translation="MRARMMRHLPLLVAVSLYLAVGFLLLPYYRYQINPDGISYINNA
                     RLLLQGELLRTVNNHWNPLISWLLAPFLLFMNDAHLAFKLMNLLIGAAGFFLLNCWLE
                     RLRINGWLRWLVLFILAPVLLWMAISVNSPDLLVSLCLLFYLTLLYRPGYFERWGNAV
                     GLGLAAGLAYLAKYYALPFIAVHLLAWHALEYRRQRRAGRRKVLRSYLIVMLIFIGVV
                     LSWMGLQALKYGQFGRGHHTSGWIFSFIDPHEKEYPLERSGLVAPPYPGATSIWDDPH
                     SLRVKPWSPWSSPADMAAYAGIVRGFFSYFLLLLGQFSPFAAGLLLIFFWFGLKKGAS
                     PLSRREAWQWLLPAGIYVSGYLLIWVEERYLWFLHLLLIVISLYLVRAWPVLRKSWAG
                     RTVLLLLLLSFAYYPVEKLVNYLGRGRNYHEMAVQLQAAHLPTLARIASNAYWNESLY
                     LCYYRGGLYYGSCTPYRDEAAVRAALQKHKIDYFLHWGRQPASRFSFLNACPELTGGG
                     IEGLRLYDLRGLSEEAAPAPEP"
     gene            2991..5438
                     /locus_tag="PKM23_01395"
     CDS             2991..5438
                     /locus_tag="PKM23_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNY90154.1"
                     /translation="MNYGYFDDEKREYVITRPDTPLPWINYLGCEEYFGIISNTAGGY
                     SFYRDARLRRLTRYRYNNVPFDLGGRLVYLRDNDSGAFWSPSWQPSRQEVGEYFCRHG
                     MGYSIIGSTSAGIRAETLFFVPLGENLEIWRVKVTNERKEPARLSLFSAVEFCLWDAQ
                     DDATNFQRNFSTGEVEVEQGVIYHKTEYRERRNHFAWFACSPKPAGFDTQRDIFLGRS
                     RGWERPAAVERGRSFNSIAHGWSPIGSHHLKLNLNPGETRQVIILLGYQENPKEDKFD
                     PPGSQIINKKRVKPVIAKYLKPREVEKAFSALRAYWDGILGLYQVKTPDKHTNRMVNI
                     WNAYQCMATFNMSRSASFYESGIGRGLGFRDSNQDLLGFVHMVPARARERVLDLAATQ
                     LPSGGAYHQYQPLTKRGNNDIGSNFNDDPLWLILGTCAYLKETGDWSILDEMVPYDNA
                     PGSETPLYEHLQRSLTYTLDRIGPHNLPLIGRADWNDCLNLNCFSDTPGQSFQTTTNK
                     EGKVAESVFIAGLFVLAANEMADLAEKRGRKPEAVRYSQAAELMEAVVKKHGWDGSWF
                     LRAYDDFGHKVGSKECKEGKIFIEPQGMCVMAGIGVGEGLAKKALDSTHKLLGTEHGI
                     MLQQPAYSRYYLELGEISSYPPGYKENAGIFCHNNPWIMIAETVIGRGDKALEAYLRI
                     NPSAREKLAELHRCEPYVYAQMIAGRDAPSHGEAKNSWLTGTAAWNYVAITQHILGIR
                     PTYDGLMVAPVIPESWKGFRASRVFQGVLYDIEVKRIGRGNAVLLTVDGEAVEGSIIP
                     RPEDGRRQVTVRVRLGG"
     gene            complement(5456..5584)
                     /locus_tag="PKM23_01400"
     CDS             complement(5456..5584)
                     /locus_tag="PKM23_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90155.1"
                     /translation="MSKFLRGLAAGWGAKKLGRGCFSTILIFLILWWLLSYIDIFK"
     gene            complement(5676..7193)
                     /locus_tag="PKM23_01405"
     CDS             complement(5676..7193)
                     /locus_tag="PKM23_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HNY90156.1"
                     /translation="MPARIGFDNEKYLAEQSAEILNRIEQFGDKLFLEFGGKLIYDYH
                     AARVLPGYDPNVKMRLLQKLADKVDIILCIYAGAIEHKKIRADFGISYDADALKLIDD
                     LREWGIDVTAVVITRFEEQPSAIQFRNKLERRGFTVYTHKTTRGYPNNIEMIVSEEGY
                     GANPYIATTRPIVVVTGPGPGSGKLATALSQLYHERQRGVRAGYAKFETFPIWNLPLK
                     HPVNVAYEAATADLRDFNMIDSHHLDAYNEKTVNYNRDVEAFPLLRRIIEKITGEPSP
                     YKSPTDMGVNRAGFGIVDDEAVQAAAIQEIIRRYFRYATEYALGLIDQGTVERAELLM
                     VEIGAKPEDRPVVLPARRAAVEAERRGKGNEGIFCGAALELADGTIITGMNSTMMHSS
                     AALILNAAKHLAGIPPAIDLLPKSIIEALTFFKRDVLEGKRLSLDLEEALIALSISAA
                     ANPAAQAAVEKLKELRGCDVHTTHIPAPGDEAGMRKLGLNLTCDPGFASRYLFMS"
     gene            complement(7662..10676)
                     /locus_tag="PKM23_01410"
     CDS             complement(7662..10676)
                     /locus_tag="PKM23_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90157.1"
                     /translation="MKRTAAVLLLVLLAGWATRSSGQINATAHWPGGNCQAVALTDDY
                     ALAAFGGFLHIVHLADYQLISTLELPDAVRDIVVYGEIAYIAAAKTGILVVDFSDPAR
                     MKLLTTLPGFAWKLIIADGRLYVAAYGSGVALFDLATPRQPLLLGALNITPVVYDVAV
                     SGRYLYCAGSSRGLTVIDQQDPAALKELVTLPASKSIFHLCLQGRYLYASFEYNEGLD
                     IFDLATPTAPQLLPPFSSTFSYYQMILSGTRMFANTYTNGINVLDLSDPLHPVEREKH
                     EIGILLDFALSGTALAAVDEWEGVSFYHRQEGSLSSATTHIELDFNALKMFTQGSTLY
                     FPIYGGGMRIYDLTDPQAPVDRGKADWYPDDMVFNSSLAYVLTAARLYILDISTPWQP
                     VQIAQATLRVSQEMLTLHNNRLYAYGGLYSGTYGCDVVDISTPAAPVVKEMIVHEPGF
                     THIEGVGNALLALDRHQQLFRIDVTDPDHPLKEPAPMLPDSVASFWLLGQTGYVTDMH
                     DRLSIYDLSDPFHPVLHATHEDANLSARPLMVGTLGYCSAFLGLNVFDFTDPYDPALR
                     GFVEVSSQFAPPALVSGRLALLKIIMEWKVTLYDLEHPLNPVPVGRLAPLGKNRGLAV
                     REALAFTANGPGGLRMIGMSGAAQQQVGEWHTHGKVEDVALQGEYAYLAVESGGMHVV
                     EIADPAHPRAVGHYYPATNGSVEAITVSGGLACLLGGVYNITILDIHQPAQPAELAKY
                     GAGWSCQGGALAGQYLYYCDGNVGVKILDLRDPAHPTLTGSLDTPGFAHNLCIRDSLA
                     YVADDRSSLRILNIADPAHPREIASHEITYAEILSVELYGPYAIVAASYSGVYILDIS
                     DPANIRQLANFYISGCSFVDVAVAGGYIYACDEQYGVYKLSSELITGLAAEEPLHPAG
                     FELAQNYPNPFNMSTVIRFRLGVQEKVSLVIHDLLGRKVRSLIQERLNAGIYLTPWDG
                     KTDGGENASSGIYFYRLKVGNHVETRRMLLVK"
     gene            11282..12049
                     /locus_tag="PKM23_01415"
     CDS             11282..12049
                     /locus_tag="PKM23_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNY90158.1"
                     /translation="MLQKNGGRMRLHILSDLHIEFGEYDPAQTDADVVILAGDIHLRQ
                     RGVEWAMSRFDGKRVIYVPGNHEYYGEALPRNAEKMKELARGSNLCVLEKDAVMIGDW
                     KFLGCTLWTDFRLHGDPRIAGYEATQKMTDYKKIRISPEYRKMSSRDTASLHARSVEW
                     LKRELSDQNAKTVVITHHAPSARSLPEKFRADLLGAAYASHLDEVVEASHAKYWVHGH
                     MHSSSDYRIGETRVICNPRGYPDELNSNFLADLLVEI"
     gene            12077..12229
                     /locus_tag="PKM23_01420"
     CDS             12077..12229
                     /locus_tag="PKM23_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90159.1"
                     /translation="MLPSWNEAREQLLAPILTANPDYFDLYWRAWELAFAHLRQPTHR
                     QLDVER"
     gene            complement(12629..12757)
                     /locus_tag="PKM23_01425"
     CDS             complement(12629..12757)
                     /locus_tag="PKM23_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HNY90160.1"
                     /translation="MQNITFLFAAYSLIWFALFLFLLRLAKKVSRLEQELHRIEEK"
     gene            complement(12766..13440)
                     /locus_tag="PKM23_01430"
     CDS             complement(12766..13440)
                     /locus_tag="PKM23_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HNY90161.1"
                     /translation="MKSIKPLYLALAVLTFLLIEGALYMALLQAPTERTMGDVQRIFY
                     FHVPSAWVSFLAFFIVCIASIAFLRTGKAQWDRLAASSAEIGILFITLVLLTGPLWAK
                     PVWNVYWTWDARLTTSLILWLIYIAYMMVRRYTSDPERGAKFAAVFGIIGFLDVPIVY
                     MSIRWWRTLHPAPVIGGGGGLAPEMKQALFFSLFAFTVLYITLLLLSLRIRSVEDKIQ
                     ALKNKS"
     gene            complement(13554..14231)
                     /locus_tag="PKM23_01435"
     CDS             complement(13554..14231)
                     /locus_tag="PKM23_01435"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNY90162.1"
                     /translation="MQTLTQFYTILHKDLLQELRSKEIITAMLVFSLTVVVIFNFVFD
                     PGSAEMRTTAPGILWVAFIFAGNLGLSRAFAREQENSMMQGLLLCPVDRSLLFTAKVI
                     GNILFIGLVEIITLPVMIIFFDLAVGRAWPLLLAILFLGTAGFATVGTIFSAISANTR
                     SREVMLPIMLFPVTVPIILAAIKSTSYLLAGRETADIWPWLRLLIGFDVLYFFVCFLL
                     YEYVVEE"
     gene            complement(14236..14925)
                     /gene="ccmA"
                     /locus_tag="PKM23_01440"
     CDS             complement(14236..14925)
                     /gene="ccmA"
                     /locus_tag="PKM23_01440"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HNY90163.1"
                     /translation="MILTRDLRKSFGPVQALAGLSFAMAEGELAALLGANGAGKSTLL
                     RLISGLSRPSSGSAEVAGFDVRRQPQEVRRRIGVVSHQTFLYEDLSAEENLHFYGRMY
                     SVAGLQERSDELLAMMELWPRRTDLVRTFSRGMQQRLALARAMLHQPTVLLLDEPFTG
                     LDLHAIDLLTSFIEEASAGRVTVLMTLHDPDYALAHSRRLFVLHQGRLVIDQPSEQVR
                     KGDILPFFREK"
     gene            complement(14922..16211)
                     /locus_tag="PKM23_01445"
     CDS             complement(14922..16211)
                     /locus_tag="PKM23_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90164.1"
                     /translation="MSIVLALLIMIAAVAWVVAPLFRDPSTAVHSPAEASASSGELLY
                     RKQVATGIINDLHFDFQTGKLSSADHAALVAEQEKLIAAIDARLEAGPARTKSQKKST
                     ARGLTAGLLLLLMTPAIPASAREVLFSGRLLDGSRDSTGVANSAVALQLLRDDGHPPR
                     EVDTQRTGAGGAFSFRIAAPDTGAAYLAMVEHQGVRYYSDQASFAGSEMARHDIVRFD
                     STRSSREIIVLMHHLFVQDLGEVLALRETRVLHNPTARTIIDAVADGHETGAILRIGL
                     PPWAQNITPIAGRFGKDLHLHGTDLYDAGVFEPGNRQISFTYELPWQRDRATLVIEVE
                     QPTRSLDLFIGQEGLRLEGEGLTSHGPFTIRGTVYQRYGIENVRPGARVQLQVARQPQ
                     DRNALSPWVTLAATASLLLLGVALAQLKNAKKETARK"
     gene            complement(16233..18209)
                     /locus_tag="PKM23_01450"
     CDS             complement(16233..18209)
                     /locus_tag="PKM23_01450"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNY90165.1"
                     /translation="MADLGYQALLIALALSLYAVIIYTGAARKSAALYLESAHRAVVT
                     TLLLVTLASAALIHAFLTRDFQLQYVAQYSNRSLSWFYTLAAFWAGQDGSLLFWTWLL
                     AIFGVIVLVTNKKRHPELLPAAMAVIQFTTLFFLYLATFKTNPFARLPFVPEDGNGLN
                     PLLQNPGMVFHPPSLYIGFVAFTVPFAFAIAALINKSLDERWIKSIRRWTLFAWLFLT
                     IGNILGMQWAYVELGWGGYWGWDPVENASFLPWLTGTAFLHSVIVQERKGMLKIWNMV
                     LVTLTFALTIFGTFVTRSGVISSVHAFGVSNLGPAFLAFLGLIIAGSAGLIISRSSLL
                     QSRGHIGSWSSKEWSFLLNNILFLALTLAVLLGTIWPTVTEAVSQQKITVGPEWFNRM
                     ATPIGLAVFALTGLCALLAWNRTTKQNLLRSLRAPLLITLAIILLIVLLGVRGPLPLF
                     ALAIAIFSISATLLEFVRGARVRKKNEATGYPGALVSLARRNKRRYGGYIVHLGVLLL
                     FLGIIGSSAFSLEKEATLRKGESLEIGRYRLLYQGMVSNERPDRRVDAARFDLFINGK
                     PAGRMEAEKHLHPNFQPATEVAIRSNLREDLYVILASYDLSSQIATVSVLINPLILWM
                     WIGGIVMTLGTLLAISPQGAGRKRDRAADGPEAA"
     gene            complement(18380..19462)
                     /locus_tag="PKM23_01455"
     CDS             complement(18380..19462)
                     /locus_tag="PKM23_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNY90166.1"
                     /translation="MKLHRLVILLNAALAGAVLAQTAPDQLLLKDYRPRSIYKVPSHH
                     IERAKFPVIDAHSHAYAQSPAEVDAWVKTMDRAGIEKTIILSYAVGAEFDSIYAVYTA
                     YPGRFEVWCGLDFSGYNQPGYGPAAVAELERCYKVGARGVGELGDKGEGLVYGRVPAP
                     GMHLDDPRMDPLLEKCADLGMPINIHVGEPIWMYQPMDSTNDGLMNGFSWRLDNKPGI
                     IDLAGMVQNLERAVKRHPRTTFIACHFANCDYDLAKVGRLLDLYPNLWCDISARYAET
                     APVPRATRAFYEKYSKRLLYGTDMGMDPAMYAITFRILESTDEHFYEIDHFNYHWPLY
                     GLGLSDKVLKRVYRTNALKALYPGRK"
     gene            complement(19459..20355)
                     /locus_tag="PKM23_01460"
     CDS             complement(19459..20355)
                     /locus_tag="PKM23_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNY90167.1"
                     /translation="MNKKLWLVYALVTTLFWGVWGALIELPEKAGFPATLGYVVWALT
                     MLVPVLVAMNNIGWKVEFDRRSVWLGSAAGFLGAGGQLILFQALRSGPAYLVFPFISL
                     SPVLTILMSYLMLKERASKRGWAGILLAMVAMPLLSYQPANSPIQGGLWIVLALLVFL
                     AWGIQAFVIKFANETMKAESIFFYMTVTGLLLIPFALWMTDFSQPINWGLKGPWLAAA
                     IQILNAIGALTLVYAFRHGKALIVSPLTNAVAPVITIILSLTLYRVIPHPLIITGMVM
                     AMAAVFLMAIEEGTEEPKGVEK"
     gene            complement(20396..21535)
                     /locus_tag="PKM23_01465"
     CDS             complement(20396..21535)
                     /locus_tag="PKM23_01465"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNY90168.1"
                     /translation="MNGKLTYNEIHRQAEIWRGATAALAAAREEHHVLLQRYQNHLWV
                     FTGCGTSYYLAQTAAALFTGLTGLRSKAVPASEILIYPELVFNPYERTVAVAFSRSGT
                     TTEIVRAVEKARNELGIPVLCVSCDPQSPLARAGLHTVTFPFPAEESVVMTGSFTTML
                     LSLMQATLQGRKMTEQAAVLDQLPDAAGAVLALADEMLPAIVAHSFNQFVFLAQGPFY
                     GLANEAALKMNEMSLSASRSFHALEYRHGPMSTTTAETLITLLASQKGVEYELQMAAD
                     MKKLGARILLLHDSSLNCLPGEVDFDLCIPGPGGDFANALLYMPVLQLLGYYNALHCN
                     QNPDRPNNLTAVVKLDLSAPTLSEEKSWSAVPDLHNLNPGLRTHA"
     gene            complement(21581..23704)
                     /locus_tag="PKM23_01470"
     CDS             complement(21581..23704)
                     /locus_tag="PKM23_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HNY90169.1"
                     /translation="MIKRTLLLTALGLLLLSSLLSAQSDPMLYQRWNTMNINRLSTQF
                     NNTGMLADGNEQNIPLARKPSFEYPTGSGINYGTCVGFVIGAPAAQDPGAVSGANSEN
                     LEYLDGSMDEGPAAFWDEEHYAPYPEVVGSALAPLSTDPSTWPKSGPNRGWPTFIPGT
                     SDSLHVGSEGWPGAGRRGERIADQETYSVMYAWRGTDLGTSERRWLNTQVEMRGLAWS
                     GELYQDFIVWIFVVRNIGTAPIRGMRVGVHSDFSYLPLAFSPTSTGDTDRHFYDPKLQ
                     FAYGTDDNAYEDHPEGGSLGANQIAWGGTMALRMPGPSKKVATYDAFNFWMEATTPSG
                     NGASKELYFRYNLANQGDPEDSNKDGIDDDFDHNGIADDLESGQSYYLGLGADGLQVM
                     GSQPFDLAPGQVDTMVFATVFGMSKAQLLKNAASALTLYESGWKVVQAPPAPRLEIIP
                     GNGRNTLLWSTESERDPEFEGYKIYRSTDDGVTWGTETFTDFSGTTKYIPLAHFDRID
                     GIKGNYRTLPEFAWFNLGSESGLPLPRVIEADTLSYFKRGDTVHVYIDDQLINGLNYR
                     YYIAAYDTGNGITGPLENTAASQPRTGSNTVTVVPHAPVARSLLDQVRVVPNPYVVAS
                     GWEVGSDKQLQFTYLPGEATIRIFNSAGEKVRTIQHNAPSALAPSIAIWDLKNEDQQL
                     VAAGLYFYYIESSLGRTQGKFLVIM"
     gene            complement(23717..25063)
                     /locus_tag="PKM23_01475"
     CDS             complement(23717..25063)
                     /locus_tag="PKM23_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY90170.1"
                     /translation="MKRNHMRMLLPLAVFAMLQAGCPNREPVAPIEITRPKEKAVIPA
                     AGTLAVPLDASIQFQFTEPMSLPSFTGRILLQSLGGSPLPGSCSQVDSTVFFKPDAPL
                     DKATLYTVELRGGIRDARMNTIEVNSTGAFSDTVVVTSTWFFSAGDYSIGGFNPLYLR
                     DRKEGSLRRFTRLDSLTGSVSGFTAPEDAVVTPDGRYIVVANTGKNEVAFVDASSLAL
                     DNKITVAATPSQVEVAGDYLYVISVNGKTISKINLQTKAIDAKFTTSFFPGKLALNPD
                     GSVLYTMDQLKRDLVLINTATGAIIKTLPAVVDKLILGEIRTDPFSGALYICDAKGLK
                     VKRSDAGGAAPQTIYTFPAGSEPIDIAFSAQYHFIAAGKSLYKYDAATGSPIASTAFA
                     TACKSVTAAPTGDVLFVTLATSMAILDARSLSLLSEVPLASSGIESVVVNPVKFTL"
     gene            complement(25085..>25516)
                     /locus_tag="PKM23_01480"
     CDS             complement(25085..>25516)
                     /locus_tag="PKM23_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90171.1"
                     /translation="TNFGGAMQFNGNDLLVTHVVSGSPTNKQVPAVLETKEWDMPMTF
                     RLGVATDFIERGDIVWSGSYAINDARDFTTRHNLGTALTLKKMLTLRAGYRINYDEVS
                     FSAGGGLLVPANFLGQLHIDYAYTDFGQFSNIHQFSMAVNF"
BASE COUNT         5121 a         7045 c         8318 g         5032 t
ORIGIN      
        1 gttgagccgg atcgatacca ccatcctcgg ctatgccacc gttcaggatg accgggggac
       61 gccctggctg gtcaccggcg gggccgaggg agagacgctg gcggcgacga ccctggagga
      121 gctgcgcgcg gggcttgaat ccccgggccc ctgtacggtc cagttcagcg gcgagatcgc
      181 cggcagcaca gtcattgcca tccagtccga caagaccctg ctcggcgtgg gcaaggcggc
      241 gcacctgcgc ggcatcgaac tggccatcaa cggagcgcgc aatgtcatca tccgcaatgt
      301 gattctctcc catgtccccg atgccggaga gaccaatgat gccatgagca taaacggcgg
      361 ttcgcaaaat atcgtcatcg accgctgcga gtttttctcc gaccgcgacc atggcaagga
      421 ttattatgac ggcctgctcg acatcaagaa ccgttcctcc tttatcaccg tctccaactg
      481 cgcctttcat gaccactgga aagcgatcct gatcagctcc ggagacaccc agtatgccga
      541 ttccctgatc cgcatcaccc tgcaccacaa ccatttctac aacatcggct cgcgcctgcc
      601 ccttatccgc ttcggcaagg cccatatttt caacaatttt taccagaact gcgacgatgc
      661 catcaacacc cgcatggggg cctgcgtgcg ggtggagcgc aactatttcg aaaatgtcgg
      721 caagggactt ttttctgact attccgcagc ggagggtttt atacagctgg tggacaacca
      781 cttcggcggc gccagctata tcaccgcccc cctttgctcg ttcgatccgc cctatgccta
      841 tgccgacagg atgcataagg tggagctgct gcccgccctc gtcccggcgc gcgcaggaac
      901 cctggtcccg gctgataaag ttgtcgccgc cccggggcgg ctgagcctgt cgcagaatta
      961 tcccaatccc ttcaatcctg tcaccctgtt gcgttattat gtgccgggca gctcttcagt
     1021 caccattacg gtctacgacc gcctgggcaa agaggtggcg accctggtgg atggcctccg
     1081 gtcgggtgaa aaccgcgtcg aattccgggg ggatgagctg cccaccggaa tctattttat
     1141 caccctgcgg gccggggtcc aggttgtgac ccgcaagatc ctgctgctca ggtgacctgt
     1201 catcctatcc ggcggggagg cgatccggaa gagcggggca agcgggaatt tcacttacag
     1261 agaggattca tgagagcacg aatgatgcgg catctgccgc tgctggtggc ggtctcgctc
     1321 tatctggccg tggggtttct cctcctgccc tattaccgtt accagatcaa tccggatggc
     1381 atcagctata ttaataacgc tcgcctgctc ctgcagggag agctgctgcg gaccgtgaat
     1441 aaccactgga atcccctgat ctcgtggctg ctggctccct ttttgctctt catgaacgat
     1501 gcccatctcg ccttcaagtt gatgaatctg ctcatcggcg cggccgggtt tttccttctg
     1561 aactgctggc tggaacggct gcgcatcaat ggatggctgc gctggctggt gctgtttatt
     1621 ctggcgccgg tgctgctctg gatggcgatc tccgtgaaca gccctgatct gctggtctcc
     1681 ctctgcctgc tcttttatct gacactgctc taccgcccgg gctatttcga aagatgggga
     1741 aatgctgttg ggttaggctt ggcagcgggt ctggcctatc tggccaaata ctacgccctg
     1801 ccctttatcg ccgtccacct gctcgcctgg catgcgctgg aataccgccg tcagcggagg
     1861 gccggccgcc gcaaggtgct gcgcagctac cttatcgtca tgctgatctt tatcggcgtg
     1921 gtactgagct ggatgggcct gcaggccctc aagtatggcc agttcggcag ggggcatcac
     1981 accagcggct ggattttctc cttcatcgat ccgcacgaga aggaataccc gctcgaacgc
     2041 agcggtctgg tggcaccgcc ttatccggga gcaacgagca tctgggatga tccccattcc
     2101 ctgcgggtca agccctggtc gccctggagt tcaccggccg atatggcagc ctatgccggg
     2161 attgtgcggg gctttttttc ctattttctc ctgctgttgg gccagttttc cccctttgca
     2221 gcagggcttt tgctcatctt tttctggttt ggactgaaga agggggcctc gcccctctcc
     2281 cgccgggagg cctggcagtg gctgctgccg gccgggatct atgtatcggg atacctgctg
     2341 atctgggttg aggagcgcta cctctggttt cttcatctcc tgctgatcgt gattagtctg
     2401 tacctggtca gggcatggcc ggtgctgcgg aaaagctggg ccggccggac ggttttgctc
     2461 ctgcttctgc tctccttcgc ctactacccg gtggaaaaac tggtcaatta tctcgggcgc
     2521 ggccgcaatt atcacgaaat ggcggtgcag ctgcaggcag cgcatctccc gacgctggcg
     2581 cggatcgcct ccaatgccta ctggaacgag agcctctatc tctgttatta tcgcggcggg
     2641 ctctactacg gcagctgcac accctaccgt gatgaggccg cggtgcgcgc tgccctgcag
     2701 aagcacaaaa tcgattattt tctgcattgg ggccgtcagc ctgcctcccg gttttcattt
     2761 ctgaacgcct gtcccgagtt gaccgggggc ggcatcgaag ggctgcgact ctatgatctg
     2821 cgcggcctgt ccgaagaggc tgctcctgcg ccagagccgt gagcccggca ggcggcggag
     2881 agcgccgatg cgcgattttt cttgatttta cgctcctgat tggctaattt tgccggcgga
     2941 agcagatcta aaccagtacc tcctccttat ccaataaacc cggagctgcc atgaactacg
     3001 gctatttcga cgacgagaag cgtgaatatg tcatcacccg gcctgatacc cccctgccct
     3061 ggatcaacta tctcggctgt gaagagtatt ttggcatcat ctccaacacc gccggcggct
     3121 actcctttta ccgcgacgcc cgcttgcgca ggctgacgcg ctaccgctac aacaatgtcc
     3181 ccttcgatct gggcggacgg cttgtctacc tgcgcgacaa cgacagcggc gccttctggt
     3241 cgccctcgtg gcagccgagc cggcaggaag tcggggagta tttctgccgc cacggcatgg
     3301 gctacagcat catcggctcc acctctgccg gcatccgggc cgagaccctt ttctttgttc
     3361 ccctgggaga gaatctggaa atctggcggg tgaaggttac caacgagcgc aaggaaccgg
     3421 ccaggctctc gctcttttcg gcggtggaat tctgcctctg ggatgcccag gatgacgcga
     3481 ccaatttcca gcgcaacttc agcaccggcg aggtggaggt cgagcagggg gtgatctacc
     3541 acaagaccga ataccgtgag cgccgcaatc attttgcctg gttcgcctgt tctcccaagc
     3601 cggccggttt cgacacccag cgcgacatct ttctcggccg cagccgcggt tgggaacggc
     3661 cggcggccgt cgagcgcggc cgctcattca attccatcgc tcacggctgg tcgccgatcg
     3721 gctcccatca tctcaagctc aatctcaatc ccggcgaaac caggcaggtg atcatcctgc
     3781 tgggctatca ggagaacccg aaagaggata aattcgatcc cccgggctcg cagatcatca
     3841 acaagaaaag agtcaagccg gtcatcgcca aatatctcaa gcccagggag gtggagaagg
     3901 ccttctcggc cctgcgcgcc tattgggacg gtatcctcgg cctctaccag gtcaagaccc
     3961 ccgacaagca taccaaccgc atggtcaata tctggaatgc ttaccagtgc atggccacct
     4021 ttaatatgtc gcgctcggcc tcgttctatg aatcgggcat cggccgcggc ctcggtttcc
     4081 gcgactccaa ccaggatctt ctcggcttcg tacatatggt gccggcgcgc gcgcgggagc
     4141 gggtgcttga tctggcggcg acccagctgc ccagcggggg ggcttatcat cagtaccagc
     4201 ccctcaccaa gcgcggcaac aacgatatcg gcagcaattt caacgacgat cccctctggc
     4261 tgatcctcgg cacctgcgcc tatctcaagg aaaccgggga ttggtcgatc ctcgatgaga
     4321 tggttcccta cgacaatgca cccggcagtg aaaccccgct ctatgaacac ctccagcgca
     4381 gcctgaccta caccctcgac cgcatcggcc cccacaatct gccgctgatc ggccgcgctg
     4441 actggaacga ctgcctcaac ctcaactgct tctccgacac gcccgggcaa tcctttcaga
     4501 ccaccaccaa caaggagggc aaggtggcgg agtcggtctt catcgccggg ctctttgtgc
     4561 tggcggccaa cgagatggcc gacctcgccg aaaagcgcgg ccgcaagccg gaggcagtgc
     4621 gatacagcca ggccgccgag ctgatggagg ctgtggtgaa aaaacacggc tgggatggca
     4681 gctggtttct acgcgcctat gatgattttg gtcacaaggt ggggtcgaag gagtgcaagg
     4741 agggcaaaat cttcatcgaa ccgcagggga tgtgtgtgat ggcggggatc ggggtcgggg
     4801 agggattggc caaaaaggcg ctggattcaa cccacaaact gctcggcacg gagcacggga
     4861 tcatgctgca gcagccggcc tattcccggt actatctgga gctgggggaa atctcttcct
     4921 atcctcccgg ctacaaggag aacgccggca tcttttgtca caacaatccc tggatcatga
     4981 tcgccgagac ggtgatcggg cgtggggaca aggccctcga ggcctatctc cgtatcaatc
     5041 cctccgcccg tgaaaagctg gcggagctgc accgctgcga gccctatgtc tacgcccaga
     5101 tgatcgccgg gcgggatgcg ccaagtcatg gcgaggccaa gaactcctgg ctgaccggca
     5161 cggcggcgtg gaactatgtc gcgatcaccc agcatattct cgggatccgt cccacctatg
     5221 atgggctcat ggtggctccg gtcatcccgg agtcctggaa aggattcagg gcgagccggg
     5281 tttttcaggg tgtgctttat gacatcgagg tcaaacgcat cggccgcggc aatgccgtgc
     5341 tgctgacggt ggatggcgag gcggttgagg gcagcatcat ccccaggccg gaggatggcc
     5401 gcaggcaggt gacggtgcgg gtgcggctgg gggggtgagc ccggccgccg ccggtttatt
     5461 taaagatatc gatgtagctt agcagccacc agaggattaa aaagatcagg atggtggaga
     5521 aacatccccg ccccagtttt ttggctcccc agcctgctgc gagtccccgc aagaatttgc
     5581 tcatttcaat tctcctttta ttctagccat ctctattgac aaatcatctc cgtcactatt
     5641 tccttccatg cccgctttcg cggcgggcaa ttccttcagc tcatgaagag gtagcggctt
     5701 gcgaatcccg gatcgcaggt cagattgagg ccgagcttgc gcatacccgc ctcgtcgccc
     5761 ggggccggga tatgggtggt atggacgtcg cagccgcgga gttccttgag tttttcgacc
     5821 gcggcctggg cggcgggatt ggcggcggcg ctgatgctga gggcgatgag cgcctcctcg
     5881 aggtcgaggc tgaggcgctt gccttccagc acgtcgcgct tgaaaaaggt aagggcctcg
     5941 atgatgcttt tgggcagcag atcgatggcg ggggggatcc cggccaggtg tttggcggcg
     6001 ttgaggatca gggcagccga ggagtgcatc atcgtcgagt tcatgccggt gatgatggtt
     6061 ccgtcggcga gctcgagggc ggcgccgcaa aagattccct cgttgccctt gccgcggcgc
     6121 tcggcctcca cagccgcccg gcgggcgggc aggacgaccg ggcggtcttc cggtttggcg
     6181 ccgatttcga ccatgagcag ctccgcccgt tccaccgtcc cctggtcgat gagaccgagg
     6241 gcgtattcgg tggcgtagcg gaaatagcgg cggatgatct cctgaatggc agcggcctgg
     6301 acagcctcat catcgacaat gccaaagccg gcccggttaa cccccatatc tgtgggcgac
     6361 ttgtagggcg agggttcgcc ggtgattttt tcgatgatcc tgcgcagcag gggaaatgct
     6421 tcgacatcac gattatagtt gacagttttt tcgttgtagg catcgaggtg gtgtgagtcg
     6481 atcatgttga aatcacgcag gtcggcggtc gcagcttcat aggcgacatt gaccggatgc
     6541 ttgagcggca ggttccagat cgggaaggtc tcaaatttgg catagccggc gcggacgccc
     6601 cgctggcgtt cgtgatagag ctgggagagg gcggtggcga gcttgccgct ccccggcccg
     6661 gggccggtga cgaccacgat cggcctggtg gtcgcgatat atgggttggc cccgtagccc
     6721 tcttcgctga cgatcatctc gatattgttg ggatagcccc tggtcgtctt gtgggtatag
     6781 acggtgaagc cccggcgttc aagcttgttc ctgaactgga tggcggaggg ctgttcctcg
     6841 aagcgggtga tcaccaccgc ggtcacatcg attccccatt cgcgcaggtc gtcgatgagt
     6901 ttcagggcgt cggcgtcgta ggagatgccg aaatccgcgc ggattttctt gtgctcgatg
     6961 gcccctgcgt agatgcagag aatgatatcg accttatcgg ccagtttctg cagcaggcgc
     7021 atcttgacgt tgggatcata gccgggcaga accctggcgg cgtgatagtc atagatcagc
     7081 ttgccgccga attcgagaaa gagcttgtcg ccgaactgct cgatgcggtt gagaatttcc
     7141 gccgattgtt ccgcgaggta tttttcgttg tcaaagccga tcctggccgg catggtcctc
     7201 tttctcccgg atattgcagt ctggtgatgg tccggtttcc tggacacggg gaaggaatcc
     7261 accccccatg aataatcatt cattatagaa aaaaccggcc tgaaaagcaa gtggaattgc
     7321 tgccgggtac ccccgaaatc tggctcccac ctgacgcctg gcgccgcgta ctccaatccc
     7381 tccataccat ttccagccta ctcacagcct gtgccctctt catgaggcca tttattttac
     7441 aaggtattta tggcagataa cattctattt attaattaaa tatctttggg tgggaacgag
     7501 atttcggggg gtagcctgga attgctgccg gcaggtgcgg gatttatctc ccgctgggtg
     7561 agggcgtaca aaaaaatggc tgacggcgat gtgccactgt cagccatttt tatcaattgt
     7621 caggaaagag ggaatggcag aaggagcctg ttattgcggc atcacttgac aagcagcatg
     7681 cgtctggttt ccacatggtt gcccaccttg agccggtaaa aatagatgcc ggaagaggca
     7741 ttctcgccgc catccgtttt cccgtcccat ggcgtcaaat agatccctgc attcagccgc
     7801 tcctggatca ggctgcgaac cttgcgtccg agaagatcat ggatcaccag gctgactttc
     7861 tcctggacgc caagccggaa gcggatcacc gtgctcatgt tgaagggatt gggatagttc
     7921 tgcgccagct cgaaacctgc ggggtgtaac ggctcttctg ctgccagtcc ggtgatgagc
     7981 tcggaggaga gcttgtagac gccatattgt tcatcgcagg catagatata gccgccggcc
     8041 acagccacat cgacgaaaga gcagccgctg atgtaaaaat tcgccagctg gcggatgttg
     8101 gcgggatcgc tgatatcgag aatgtagacc ccggagtagg aggccgcgac gatggcataa
     8161 gggccgtaca gctcgacgga gaggatctct gcgtaggtga tttcgtgcga ggcgatctcg
     8221 cggggatggg cgggatcggc gatgttcaga atccgcagcg agctgcgatc gtcggcgaca
     8281 taggcgaggc tgtcgcgaat gcaaagatta tgggcgaagc cgggggtgtc gagactgccc
     8341 gtcagagtgg gatgggccgg atcgcggaga tcgaggatct tgacgccgac attgccgtcg
     8401 cagtaataga gatactgacc ggccagggcg ccgccctggc agctccagcc ggcgccatat
     8461 ttggccaatt cggctggttg cgcgggttga tgaatatcga gaatcgtgat gttatagaca
     8521 ccacccagca ggcaggcgag tcctccactg acggtgatgg cctcgaccga accgttggtg
     8581 gccggatagt aatggccgac ggcccgagga tgggcgggat cggcgatctc caccacatgc
     8641 atgccgccgg attccacggc gaggtaggca tattcgccct gcagggccac atcctcgacc
     8701 ttgccgtggg tgtgccactc cccgacctgc tgctgagccg cgccggacat accgatcatg
     8761 cgcagcccgc ccggtccatt cgccgtaaag gccagcgcct cccgcaccgc caaaccgcgg
     8821 ttcttgccca gaggcgccag ccgtcccacc ggcacaggat tgaggggatg ctccagatca
     8881 tagagggtca ccttccattc catgatgatc ttcagcagcg ccaggcggcc gctgaccagg
     8941 gcaggagggg cgaattgaga ggagacctcg acaaaacccc gcaaggctgg gtcgtaggga
     9001 tcggtgaaat cgaagacatt caaaccgagg aaggcggagc aatagcccag tgtgcccacc
     9061 atgagcggcc gggctgagag gttggcatcc tcatgcgtgg cgtgcagcac cggatgaaag
     9121 ggatcggaga ggtcataaat ggagagacgg tcgtgcatgt cggtcacata gccggtctga
     9181 ccgagcagcc agaaggaagc cacggaatcg ggaagcatgg gcgccggttc cttaagcggg
     9241 tgatccggat cggtgacatc gatgcggaaa agctgctggt gacggtccag ggccaggagg
     9301 gcattaccca caccctcgat atgagtaaat ccaggctcat ggacgatcat ctccttgact
     9361 acaggggctg caggcgtgga aatatcgacc acatcgcagc cataagtgcc gctgtagagc
     9421 ccgccgtagg cgtagagccg gttgttgtga agggtgagca tctcctggct gacccgcaaa
     9481 gtggcttgcg cgatctgcac aggctgccag ggcgtggaga tgtccagaat gtagaggcga
     9541 gcggcggtca gcacgtacgc aagggagcta ttgaaaacca tgtcatcggg gtaccaatca
     9601 gctttgccgc gatcgacggg cgcctggggg tcggtcagat catagatcct catcccgccg
     9661 ccgtaaatcg gaaaatagag ggttgaaccc tgtgtgaaca ttttcaaggc attgaaatcg
     9721 agctcgatat gggtcgtcgc gctgctgagg ctgccctcct gtcggtggta aaaggaaacc
     9781 ccttcccatt catccacggc ggccagggcg gttccgctca gggcgaaatc caggaggatg
     9841 ccaatttcat gcttttcccg ttccaccgga tggaggggat cgctgaggtc gaggacgttt
     9901 atcccgttag tgtaggtgtt ggcgaacatc cttgtgccgg agaggatcat ttgataatag
     9961 ctaaaggtgg agctgaaggg tggaagcagc tggggggcgg tgggtgtcgc aagatcaaaa
    10021 atatcgagac cttcgttgta ttcaaaactg gcgtagaggt aacggccctg gaggcagaga
    10081 tgaaagatgc ttttggaggc agggagtgta accagctcct tcagcgccgc tgggtcctgc
    10141 tggtcaatga ccgtcaggcc ccgcgaactg ccggcgcagt agaggtagcg gcccgagacc
    10201 gccacatcat agaccaccgg ggtgatattg agggcgccga gcaggagcgg ctgccgcggt
    10261 gtggcgaggt cgaagagggc gactccgctg ccgtaggccg cgacatagag gcgtccgtca
    10321 gcaatgatca atttccacgc gaatcccggc agggtggtga gcagcttcat ccgggctgga
    10381 tctgaaaaat cgaccaccag gatgccggtt ttggctgccg ctatataagc gatctctcca
    10441 tagacgacaa tgtcgcggac cgcatccggc agctcgaggg tggagatcag ctggtagtcc
    10501 gccagatgga cgatgtgaag gaacccacca aaggcagcca gggcgtaatc atcggtcaat
    10561 gcgaccgcct ggcagttgcc gcccggccag tgggcggtgg cgtttatttg gccggaagaa
    10621 cgcgtagccc atccggccag caaaaccagc aacagaacgg cggcggttct cttcatacag
    10681 gtcccctggc aatatgaaat ggcgattgga tcggttggtg agagtgatgg taaaacagta
    10741 tggcgagcga aatccccttg ggtgtgcact cccccgtgag cctctctttc agtataaaga
    10801 atcaacttaa aaatagcaag taaaaacaac tatttctggc ggtggatggg cagggctgct
    10861 cgagcgtggt gagatcgaat ggtagggcca ttggcaactt tttgactatt ttgccggcgg
    10921 gcatgcggag gccgacagct ggattcaggg aatcctttgc ctttcaatag ctgtttgact
    10981 atattaccga aaaagaacgt attcgatcca gccgggatcg gcagtgacca ggatatacga
    11041 gaccatgagc aattcgcttg atttctgttg cagtgggccc gaatagctac attgtatgtc
    11101 tgttttacag gttgtttgca ttctttcatc cgcattaaca aatgcaggta aattggtcat
    11161 gagcgctgtc ccgttatcca ttcctttaga cggctttccc atttagccgg ctgcaggggc
    11221 gacctcggcg cagaaatccc cgaggacgtg gtcaggctga tcgcgcagca ttggatccag
    11281 tatgctgcag aagaatggtg gcaggatgag gctgcatatc ttgagtgacc tacacatcga
    11341 attcggtgag tatgatccag cccagacgga tgcggacgta gtcattctgg ccggtgacat
    11401 ccacctccgg cagcgaggcg tcgagtgggc catgtcccga ttcgacggca agcgggttat
    11461 ctacgtgcct ggcaaccacg aatactacgg tgaggcgctg ccaaggaatg cagaaaaaat
    11521 gaaggagctg gcgcgaggct ccaacctttg tgtgcttgaa aaggacgccg tcatgatcgg
    11581 cgattggaaa ttcctggggt gcacactatg gactgatttc aggctccacg gcgatccaag
    11641 aattgcgggc tacgaggcga cacaaaagat gaccgactat aaaaaaatcc gtatcagccc
    11701 cgaataccgc aagatgagtt cgcgcgacac agccagtttg catgcaagat cggttgaatg
    11761 gctcaagaga gaactttcgg atcagaatgc gaagaccgtt gtgattactc accatgcgcc
    11821 aagcgcgagg tctttgcccg agaagttccg agcggatctg ttgggggcag cgtatgcgtc
    11881 gcacctcgac gaggtggtcg aggcatctca tgcgaagtat tgggtacatg gccacatgca
    11941 ttcaagcagc gactaccgga tcggagagac aagggtgatc tgcaatcccc gtggctaccc
    12001 cgatgagttg aattccaact ttctggctga ccttctcgtc gagatatgat aggccgggaa
    12061 agcatatgtc gaaaccttgc tcccctcctg gaacgaagcc agggagcagt tgctggcgcc
    12121 catcctgacg gcgaatcccg actatttcga tttgtactgg cgcgcctggg agctcgcctt
    12181 cgcgcatctg cgccagccga cgcatcgaca gcttgatgtg gaacgataag gatggcctct
    12241 attatgatct cgatgactct ggcgaacagc tgaaatgcaa aactcttgct tgtttctggc
    12301 tgttggtggc cgggttagcc gatgaggcta aagcacaaaa tctgctggcc aacctcaaaa
    12361 atcccgaaac cttttggtgc ccaacccctt ttccctccct ggcggcgcat gaaccgattt
    12421 ataaagcgga tggccagtat tggcaagacg gagtctgggc gccaaccaac gtgatgatca
    12481 tcaagggcct cggccgcttc ggctggccgg cttgtgacga acagcgcgtg tcgtccttac
    12541 gcggcagcca gggggcgatc tgcttcagca cgccgggtcc gaaggggatg cgacctgcgg
    12601 tggcgaattc gaaattcatt caccccggct atttttcctc gatacggtgg agttcctgct
    12661 ccagccggct cacttttttg gccaggcgca gcaggaagag aaaaagggcg aaccagatca
    12721 gggaataggc ggcaaagaga aaggtgatgt tctgcattag ctccctcagg atttattttt
    12781 cagtgcctgg attttgtctt ccacgctgcg gatgcgcagg gagagcagca gcagggtgat
    12841 gtagagcacg gtgaaggcga acaaagagaa gaaaagagcc tgtttcatct ccggggccag
    12901 tccgcccccg cctccgatga ccggagcagg atggagggtg cgccaccagc ggatcgacat
    12961 gtagacgatg ggaacatcga gaaagccgat gatgccgaag accgcggcaa atttggcgcc
    13021 gcgctcgggg tcgctggtat atctgcgcac catcatataa gcgatgtaga taagccagag
    13081 gatcagggat gtggtcagcc gggcatccca tgtccagtag acattccaga ccggcttggc
    13141 ccacaatggc ccggtcagca gcaccagggt gatgaagagg atgccgattt cggccgagct
    13201 ggcagcgagg cggtcccact gcgccttgcc agtgcgcaga aaggcgatgc tggcgatgca
    13261 gacgatgaaa aaggcgagga aggagaccca ggccgatggg acgtgaaaat aaaaaatgcg
    13321 ctgtacgtcc cccatggtgc gctcggtcgg ggcctggaga agggccatgt agagcgctcc
    13381 ctcgatgagg aggaaggtca gcactgccag cgcgagatag aggggtttga tcgatttcat
    13441 ccggaatcct ttaaccgatg tatcatttca gacttgtagt cgtcactttt ggcgcccagc
    13501 cgaatccggg tgggcacgag atggcccggc cgggggggtg cgagagttcg tcattattct
    13561 tccactacat actcatagag caggaagcag acgaagaaat agagcacatc gaagccgatg
    13621 agcaggcgca gccagggcca gatatcggcg gtttcgcggc cggccagaag ataggaggtg
    13681 ctcttgatgg ccgcgaggat gatcggcacg gtaaccggga aaagcatgat cggcagcatc
    13741 acctcgcgcg agcgggtgtt ggccgaaatc gccgaaaaga tggtcccgac ggtggcgaag
    13801 cccgccgtgc cgaggaagag aatggcgagc agcaggggcc aggcgcggcc aacggcaagg
    13861 tcgaagaaga tgatcatcac cggcagggtg atgatctcga ccaggccgat gaagaggatg
    13921 ttgccgatga ccttggctgt gaagagaagg ctgcggtcga cgggacagag cagcagcccc
    13981 tgcatcatgc tgttctcctg ctcgcgtgcg aaggcgcggc tgaggccgag attgccggca
    14041 aagataaagg ccacccagag gatgccgggg gcggtggtgc gcatctccgc cgagcccgga
    14101 tcgaagacaa agttaaagat caccaccacg gtgagggaga aaaccagcat ggcggtgatg
    14161 atctccttgc tgcgcagctc ctgcaggagg tccttgtgga gaatggtgta gaactgggtc
    14221 agggtctgca tgggctcact tctccctgaa gaaggggagg atatcccctt tgcgcacctg
    14281 ctccgagggc tggtcaatga ccaggcggcc ctggtggagc acgaagaggc gccggctgtg
    14341 ggccagggcg taatcgggat cgtgcagggt catcagcacc gtcaccctgc cggcgcttgc
    14401 ctcctcgatg aagctggtga gcaggtcgat ggcatggagg tcgagcccgg tgaagggctc
    14461 gtcgaggagc agcacggtcg gctggtgcag catggcgcgg gccagggcga ggcgctgctg
    14521 catgccgcgg gagaaggtcc gcaccagatc cgtccggcgc ggccacagct ccatcatcgc
    14581 caatagctcg tcgctgcgct cctggaggcc ggccacgctg tacatgcgac cgtaaaagtg
    14641 caggttctcc tccgccgaaa gatcctcgta gaggaaggtc tgatgactga ccacgccgat
    14701 gcgccggcgc acctcctgcg gctgccggcg cacatcgaac cccgccacct cggcgctgcc
    14761 ggaggaaggg cggctgagtc cggagattag gcgcaggagg gtggattttc ccgccccgtt
    14821 ggcgcccagc agggcggcca gctcgccttc agccatggcg aaggagaggc cggccagggc
    14881 ctgcaccggt ccgaaggatt tgcggaggtc gcgggtgagg atcacttgcg cgcggtttcc
    14941 tttttggcgt ttttaagctg cgccagggca acgcccagca gcaggagcga agcggtggcc
    15001 gccagggtca cccaggggga aagggcgttc ctgtcctgcg gctgccgcgc cacctgcagc
    15061 tgcacgcggg cgccgggacg gacgttctcg atgccgtagc gctgatagac ggtgccgcgg
    15121 atggtgaagg gaccgtggct ggtcaacccc tcgccctcca gacgcagccc ttcctggccg
    15181 atgaagaggt cgagggagcg ggtgggctgc tccacctcga tcaccagggt ggcgcggtcg
    15241 cgctgccagg gcagttcata agtgaaggag atctggcggt tgcccggttc gaagaccccg
    15301 gcgtcataaa ggtcggtgcc atggagatgg aggtccttgc cgaagcggcc ggcgatgggg
    15361 gtgatgtttt gcgcccaggg cggcagtccg atcctgagga tggcgccggt ctcatggccg
    15421 tcggcgaccg catcgatgat ggtcctggcg gtcggattat ggagaacgcg ggtttcgcgc
    15481 agggcaagga cctcgccgag gtcctgaacg aagagatggt gcatcagcac gatgatctcg
    15541 cggctgctcc gggtggagtc gaagcggacg atgtcatggc gcgccatttc agaccccgcg
    15601 aaggaggcct ggtcgctgta atagcgcact ccctggtgtt cgaccatggc cagataggct
    15661 gcgccggtgt ctggggcggc gattctgaag gagaaagcgc cgccggcgcc ggtgcgttgg
    15721 gtatcgacct cgcgcggggg atggccgtca tcgcgcagga gttgcaaggc cacagccgag
    15781 tttgccacgc cggtgctgtc gcggctgccg tcgagaaggc ggccggagaa gagcacctcc
    15841 cgggccgatg cgggaatggc gggggtcatc agcaggagta aaagccccgc cgtcaggcct
    15901 cttgcagtgg actttttctg ggatttcgtc cgggccggcc cggcctccag acgggcatcg
    15961 atggcggcga tgagcttctc ctgctccgcc acaagcgcgg catggtccgc gctgctcaac
    16021 ttgccggttt gaaagtcgaa atgaagatca ttgatgatgc ctgtcgccac ctgtttgcgg
    16081 tagagcagct ccccggagga ggccgaggcc tcggccgggg agtgaaccgc ggtggatggg
    16141 tcacggaaga gcggcgccac cacccaggcg acggcggcga tcatgatcag caatgccagt
    16201 actatgctca taatgcctca atttgttcca gttcaggcgg cttccggacc gtcggcggcc
    16261 cgatcccttt tccggcccgc tccctgcggc gagatcgcca gcagagtgcc cagggtcatg
    16321 acgatgccgc cgatccacat ccagaggatg aggggattga tcagcacgct caccgtcgcg
    16381 atctggctgc tcaggtcata gctggcgagg atgacgtaga ggtcctcgcg cagattggag
    16441 cggatcgcca cctcggtggc gggctgaaaa ttgggatgaa gatgcttctc cgcctccatc
    16501 cggccggcgg gtttgccgtt gatgaagagg tcgaagcggg cggcatcgac gcggcggtcg
    16561 ggccgctcat tgctgaccat tccctgatag agcaggcggt agcggccgat ttcgaggctc
    16621 tctcccttgc gcagggtggc ctctttttcc aggctgaagg ccgaggaacc gatgatgccg
    16681 aggaagagca gaagcactcc aaggtggacg atatagccgc cgtaacggcg cttgttcctg
    16741 cgcgccaggc tgaccagtgc gccggggtag ccggtggcct cgttcttttt acgcacccgg
    16801 gctccgcgca caaactcaag cagggtggcg ctgattgaaa agatggcgat ggccagggcg
    16861 aagagcggca gcgggccgcg taccccgagg agcacgatta aaaggatgat ggccagggtg
    16921 atgagcagcg gcgcgcgcag actccggagc aggttttgtt tggtggtgcg gttccaggcg
    16981 agcagggcgc agaggccggt gagggcgaag acggcgaggc cgatgggggt ggccatgcgg
    17041 ttgaaccact ctggccccac ggtgatcttt tgctggctca ccgcctcggt gaccgtcggc
    17101 cagatcgtgc cgagcaggac tgcaagggtc agggcgagaa agaggatatt gttgagaaga
    17161 aagctccact ccttggagct ccagctgccg atatggcccc tgctctgcag cagggagctg
    17221 cggctgatga tgaggccggc cgagccggcg atgatcagac caagaaaggc gagaaaggcg
    17281 gggccgagat tggagacgcc gaaggcgtgc accgaggaaa tgacgccgct gcgggtgaca
    17341 aaggtgccga agatggtcag ggcgaaggtg agggtcacca ggaccatgtt ccagatcttg
    17401 agcatgccct tgcgttcctg aacgatgacg gaatggagaa aggccgtgcc cgtcagccag
    17461 ggcaggaagg aggcgttctc gacagggtcc catccccagt aaccgcccca gcccagttcg
    17521 acataggccc actgcatgcc gaggatattg ccgatggtca ggaagagcca ggcgaagagg
    17581 gtccagcgcc ggatgctttt aatccagcgc tcatccaggc ttttgttgat cagggcggcg
    17641 atggcgaagg cgaaggggac ggtgaatgcg acgaagccga tgtagaggct ggggggatga
    17701 aagaccatgc cgggattctg cagcaaggga ttgaggccat tgccgtcttc gggcacgaag
    17761 ggcagccggg cgaagggatt ggtcttgaag gtggccagat agagaaaaaa gagggtggtg
    17821 aactggatga ccgccatggc ggccggcagc agctccgggt ggcgtttctt gttggttacg
    17881 agaacaatga cgccgaagat ggccagcagc caggtccaga agagcagaga gccgtcctgg
    17941 ccggcccaaa aggcggcgag ggtataaaac cacgagagcg agcggttcga gtactgggcg
    18001 acatactgaa gctgaaaatc gcgcgtgaga aaggcatgga tcagcgcggc cgaggccagg
    18061 gtgacaagca ggagagtagt gacgacggcg cggtgggcgc tttcgaggta gagggcagcg
    18121 ctcttgcggg ccgcaccggt atagatgatc acggcgtaga gggagagcgc cagggcgatg
    18181 agcagtgctt gataaccgag atctgccaag cgatggtcct gtctgaatgg aatgagtttg
    18241 ccagttaaat gaaacagaaa tatatcgaat atagagctta accgcaataa aaaaagccgg
    18301 cgggagccgg cttgcacgca ccttgtcgaa aatgtcgggc cgcagctttc cctgaagccg
    18361 cggctcgaaa cagcctcgcc tacttccgcc cgggatagag ggccttgagg gcgttggtcc
    18421 gatagacgcg tttgaggacc ttgtcgctca gtcccagacc gtagagcggc cagtgataat
    18481 tgaaatgatc gatttcataa aagtgctcat cggtcgactc gaggatgcgg aaggtgatcg
    18541 cgtacatggc gggatccatc cccatgtcgg tgccgtagag aaggcgtttt gagtatttct
    18601 cataaaaggc gcgagtggcg cgcgggacgg gcgcggtctc ggcatagcgg gcggaaatgt
    18661 cgcaccagag attggggtaa aggtcgagca gcctgcccac cttggcgaga tcataatcgc
    18721 agttggcgaa atgacaggcg atgaaggtgg tgcggggatg gcgcttgaca gctcgttcca
    18781 ggttctggac catgccggcg aggtcgatga tgcccggctt gttgtcgagg cgccagctga
    18841 agccgttcat gagtccgtcg ttggtcgaat ccatcggctg gtacatccag atcggttcac
    18901 cgacatggat gttgatcggc ataccgaggt cggcgcactt ttcgagcagg gggtccatgc
    18961 gcggatcatc gaggtgcatg cccggcgccg ggaccctgcc gtagaccagc ccctccccct
    19021 tgtcgccaag ctcaccgacg ccgcgcgcgc ctaccttgta gcagcgctcc agttcggcga
    19081 cggcagccgg gccgtatcct ggctggttgt agcccgagaa atcaagaccg caccacacct
    19141 cgaagcggcc gggataagcg gtgtagaccg cgtagatcga atcgaactcg gcgccgacgg
    19201 cataggagag aatgatggtt ttttcaatcc cggcccggtc catggtcttc acccaggcat
    19261 cgacttcagc gggagactgg gcgtaggcat gggaatgggc atcgatgacc ggaaatttgg
    19321 cgcgctcgat gtgatgggac ggcaccttgt agatggaccg cggccggtag tccttcagca
    19381 gcagctggtc aggagcagtt tgcgccagca cggccccggc gagtgcggcg ttgagcagga
    19441 tgacgagtcg atgcagcttc atttttccac tcctttcggt tcctccgtcc cctcctcgat
    19501 ggccatgaga aagaccgcgg ccatggccat caccatacca gtgatgatca ggggatgggg
    19561 aatgacgcga tagagggtga gggagaggat gatggtgatg accggggcga cggcattggt
    19621 cagggggctg acgatgaggg ccttgccgtg acggaaggca tagaccaggg tgagggctcc
    19681 gatggcgttg aggatctgaa tggcggcggc cagccagggc cccttcaagc cccagttgat
    19741 cggctggctg aaatcggtca tccagagggc gaaggggatg agcagcagtc cggtgacggt
    19801 catgtagaaa aagatgctct ctgccttcat ggtctcgttg gcgaacttaa tcacaaaggc
    19861 ctgaatgccc caggcgagga agaccagcag ggcgaggaca atccagaggc cgccctgaat
    19921 cgggctgttg gcgggctggt aggagagcag gggcatggcc accatggcca ggaggatgcc
    19981 ggcccagccg cgtttgctgg cgcgctcctt gagcatgaga taggacatga gaatggtcag
    20041 caccggcgac agcgagatga agggaaagac caaataagcc gggccgctgc gcagggcctg
    20101 gaaaaggatg agctgacctc cggccccgag aaagcccgcg gccgaaccaa gccagaccga
    20161 gcggcggtcg aattcgacct tccagccaat gttgttcatg gcgaccagga ccgggaccag
    20221 catggtcagg gcccagacca cataaccaag ggtggctgga aatcccgctt tctctggcag
    20281 ttcaatcagg gcaccccaga caccccagaa cagggtggtg accagggcgt aaaccagcca
    20341 taatttttta ttcactgcag ttctccttgt atggattcat gtgagggctg ccttttcagg
    20401 catgggtacg gaggccggga ttgaggttgt gcagatccgg cactgcggac caggatttct
    20461 cttcgctcaa ggtcggcgcc gaaagatcga gcttgacgac cgcagtgaga ttattggggc
    20521 ggtcgggatt ctgattgcag tgcagggcat tgtaatagcc gagcagctgc aggaccggca
    20581 tgtagaggag ggcattggcg aaatcgccac ccgggccggg gatgcagaga tcgaaatcga
    20641 cctcgccggg taaacagttc aggctgctgt cgtgcagcag caggatccgc gccccgagtt
    20701 tcttcatgtc cgctgccatc tgcagctcat actccactcc tttttgtgaa gcgaggaggg
    20761 tgatcagggt ctcagcggtg gtcgtcgaca tcggaccatg gcggtactcg agggcgtgaa
    20821 aactgcggga ggcggagagg gacatttcat tcatcttgag ggccgcctca ttggccagac
    20881 cataaaaggg gccctgggcg agaaagacga actggttgaa ggagtgggcg acgatcgcgg
    20941 gcagcatctc gtcagccagg gcgaggacag ccccggctgc atcgggcagt tgatcgagaa
    21001 cggccgcctg ctccgtcatt ttacggccct gcagggttgc ctgcatcagg ctgaggagca
    21061 tggtggtgaa tgacccggtc atgaccacgc tctcttcggc cgggaagggg aaggtgaccg
    21121 tgtgcaggcc ggcgcgtgcc agcggcgatt ggggatcgca cgagacgcag aggacgggga
    21181 tgccgagttc gtttctggcc ttttcgaccg cccgcacgat ttcagtcgtg gtgccggagc
    21241 gcgaaaaggc gaccgccacg gtgcgttcgt aggggttgaa gaccagttcg ggatagatga
    21301 ggatttcgga agccggcacg gccttgctgc gcaagccggt gagccccgtg aacagcgctg
    21361 cggcggtttg ggccagataa taggaggtgc cgcagccggt gaagacccag agatggttct
    21421 ggtagcgctg cagcagtaca tggtgttctt cccgggcagc ggcgagcgcg gcggtggcgc
    21481 cgcgccagat ctccgcctgc cggtggatct cgttataggt gagtttgccg ttcatagcgt
    21541 tctttcgttt cagttatgcg gaattcccgg agcctgctgg ctacatgatc accaggaact
    21601 tgccctgtgt ccggcccagc gacgactcga tatagtaaaa atagaggccc gcggcgacca
    21661 gctgctggtc ctcgttcttc aggtcccaga tggcgatgct cggcgcgagt gcgctgggcg
    21721 cgttgtgctg gatggtgcgc accttttcac cggccgaatt gaagatccgg atggtggcct
    21781 cgccgggaag ataagtgaac tgcaactgct tgtcggagcc gacctcccag ccggaagcga
    21841 ccacgtaggg attgggcacc actctgacct ggtcgaggag tgaacgggcg accggggcgt
    21901 gcggcacgac cgtaaccgtg ttggagccgg ttctcggctg tgaggcggcg gtgttttcca
    21961 acggcccggt gatgccgttg ccggtgtcgt aggccgcgat gtaataacgg taattgaggc
    22021 cgttgatgag ctggtcgtca atgtagacgt gcacggtgtc gccgcgcttg aagtagctca
    22081 gggtgtcggc ttcgatcacc cgggggagcg gcagaccgct ctcgctgccg agattaaacc
    22141 aggcgaattc cggcagtgtg cggtaattgc ccttgatgcc gtcgatgcgg tcaaagtggg
    22201 ccagcggaat atatttggta gtgccggaga aatccgtaaa ggtttcagtg ccccaggtga
    22261 ccccgtcgtc ggtcgaacgg tagattttgt aaccttcgaa ctcgggatcg cgttcgcttt
    22321 cggtgctcca tagcagggtg ttgcggccgt tgccggggat gatctccaga cgcggagcgg
    22381 gcggagcctg gaccactttc cagccggatt cgtagagcgt cagggcgctg gcagcgtttt
    22441 tgagcagctg ggctttggac atgccgaaga cggtggcgaa gaccatggta tcgacctgac
    22501 cgggggcgag gtcgaagggc tgcgagccca tcacctgcag tccgtccgcg cccaggccga
    22561 ggtaatagct ctggccgctc tcgaggtcat cggcgatgcc gttatggtcg aaatcatcat
    22621 caatgccgtc tttgttgctg tcttcaggat cgccctggtt ggcgagattg tagcggaaat
    22681 agagctcctt gctggcgccg ttgccgctgg gggtggtcgc ctccatccag aaattaaagg
    22741 catcgtaggt ggccaccttt ttggaaggcc cgggcatacg cagcgccatg gtgccgcccc
    22801 aggcgatctg gttggccccc aggctgccgc cttcggggtg gtcttcgtag gcattgtcgt
    22861 cggtcccgta ggcgaactgc agtttgggat cgtagaaatg ccggtcggtg tcccccgtgc
    22921 tggtcgggga gaaggcaagg ggcaggtagc taaagtcgga atggacgccg acgcgcatgc
    22981 cgcggatggg ggcggtgccg atgttgcgca ccacgaaaat ccagacgatg aaatcctgat
    23041 agagctctcc ggaccaggca aggccgcgca tttcgacctg ggtattgagc cagcgtcgtt
    23101 cgctggtgcc gaggtcggtg ccgcgccatg cgtacatgac cgaataggtc tcctgatcgg
    23161 cgatgcgttc gccccggcgg cccgccccgg gccagccttc gctgcccaca tgcagggagt
    23221 cgctggttcc cgggatgaag gtgggccagc cgcggttggg cccgctcttc ggccaggtgg
    23281 agggatcggt gctaaggggc gccagggcgc tgccgacgac ctcggggtag ggggcgtaat
    23341 gctcctcatc ccagaaggcg gccgggcctt catccatcga tccgtcgaga tactcgaggt
    23401 tctccgaatt agcgccgctc accgcgccgg gatcctgggc ggccggcgcg ccgatgacaa
    23461 aaccgacgca ggtgccgtaa ttgatcccgc tgccggtggg gtactcgaag gaaggcttgc
    23521 gcgccagggg gatgttctgc tcattgccgt cagcgagcat gcccgtgtta ttgaactggg
    23581 tgctcagacg gttgatgttc atcgtattcc agcgctggta cagcattgga tcgctctggg
    23641 cggaaagcag cgagctcaac agcaagaggc ccagggccgt caacaagagt gtgcgtttga
    23701 tcatagtcct gctcgtttat agggtgaact tgacgggatt gacgacgacg gactcgatgc
    23761 cgctgctggc gaggggcact tcggagagca gcgagaggct gcgggcatcg aggatggcca
    23821 tcgaggtcgc cagggtcaca aagagcacat cgccggtggg cgccgccgta accgatttgc
    23881 aggctgtggc gaaagcggtt gaagcaatgg ggctgcctgt ggcggcgtca tatttgtaaa
    23941 gagacttgcc ggcggcgata aaatgatatt gagcggaaaa ggcgatgtca atgggctcgc
    24001 tgccggccgg aaaggtgtaa atggtctgcg gggctgcacc gccggcatcg ctccgcttca
    24061 ccttcaaccc cttggcatcg cagatataga gggcgccgct gaagggatcg gtgcggatct
    24121 cacccagtat cagcttgtcg acgaccgcgg gcagggtctt gatgatggcg ccggtggcgg
    24181 tattgatgag caccagatcg cgtttgagct ggtccatggt gtagagtacg gagccgtccg
    24241 ggttcagagc cagtttgccg gggaaaaagc tggtggtgaa tttggcgtcg atcgccttgg
    24301 tctgcaggtt gattttggat attgtcttgc cgttgacgct gatgacgtag agataatccc
    24361 cggccacctc gacctgcgag ggagtggctg cgactgttat cttgttatcc agggcgaggg
    24421 aggaggcatc gacaaaggcc acctcgttct tgccggtatt ggccacaacg atataacggc
    24481 cgtccggcgt cactacggca tcctctggcg ccgtaaagcc gctgacgctt ccggtaaggc
    24541 tgtccagacg ggtgaagcgg cggagggagc cctccttgcg gtcgcgcaga tagaggggat
    24601 tgaatccgcc gatggaataa tcgccggccg agaagaacca ggtcgaagtt acgaccacgg
    24661 tatccgaaaa ggccccggtg ctgttgactt cgatggtgtt catccgggcg tcacggatgc
    24721 cgccgcgcag ctcgacggtg tagagcgtgg ccttgtccag gggtgcgtcg ggcttgaaaa
    24781 agaccgtcga gtccacctgg ctgcagcttc ccggcagagg cgagccgccg aggctctgca
    24841 gcaagatcct gccggtgaag gagggcaggc tcatgggctc ggtgaactgg aactggatgg
    24901 aggcgtccag aggcaccgcc agggtgccgg ccgctgggat gaccgctttc tctttcgggc
    24961 gggtgatctc gatgggggcc accggctcgc gattggggca gcccgcctgg agcatcgcga
    25021 agactgccag agggaggagc atccgcatgt ggtttctttt catagttgct ctcgttcgac
    25081 agagttaaaa attcacggcc atggagaact gatggatgtt ggagaactgg ccgaaatcgg
    25141 tataggcgta atcgatgtgg agctggccga gaaagttggc cggaaccagc agaccgccgc
    25201 cggcggaaaa ggagacctcg tcgtaattga tgcggtatcc ggcgcgcagg gtgagcatct
    25261 ttttcagggt cagggcggtg ccaagattgt gccgcgtcgt aaagtcgcgg gcgtcgttga
    25321 tggcatagct gccggaccag acgatatcgc cgcgctcgat gaagtcggtg gcgacgccca
    25381 gccggaaggt catcggcatg tcccactcct tggtctcgag cacggcgggg acctgcttgt
    25441 tggtcgggct tcccgagacc acatgggtca cgagcaggtc gttgccgttg aactgcatgg
    25501 ccccgccaaa gttggt
//
LOCUS       DAOPWU010000011        25332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_1928_length_25332_cov_5.733592, whole genome shotgun sequence.
ACCESSION   DAOPWU010000011 DAOPWU010000000
VERSION     DAOPWU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25332
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1928_length_25332_cov_5.733592"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PKM23_01485"
     CDS             complement(<1..801)
                     /locus_tag="PKM23_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNY90172.1"
                     /translation="MLFTILLAAVLLFLLAWRFYGAFLDRQFSIDNANPTPSHADYDG
                     IDRVPAPKQVLLGHHFSSIAGAGPIVGPIIATAAFGWLPTLLWIILGSIFIGGVHDYT
                     ALVASIRHKARSIAEIAREQMSPLAYKLFLAFIWLTLVYVLTVFTDLTATTFVEDGGV
                     ATSSLIFMLLAMGFGLSLYRLKLSLLASSLIFVPLVLLATWIGHIIPIDAAGVASFSD
                     LSAGRFWSIALILYAFVASITPVWILLQPRDYLSSYLLYFSILGGFIGI"
     gene            complement(892..2217)
                     /locus_tag="PKM23_01490"
     CDS             complement(892..2217)
                     /locus_tag="PKM23_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNY90173.1"
                     /translation="MKKVLVLGAGLVSRPLVRYLLDQPDTQVIIASRTVSKAEALIEK
                     HPAGKAVAATVQEKEKLEALVADADVAISLLPWIYHVQVAELCLKHKTNLVTTSYVSP
                     AMRALDQQARDQGLLFLNEIGLDPGIDHMSAMKMIHEVEHKGGRVTSFESVCGGLPAP
                     EANDNPFGYKFSWSPRGVLLAGRNSAHFLKDGKDMVIENKDLFRTVAIKDVRGLGWLE
                     LYPNRDSMLYQDLYGLKDAQTIFRGTFRNPGWCETLEKVVRLGFLNDTERPEILGWTW
                     AQLTASLINAKPEGLKAAMAKHLELPEVHDVIKRFEWLGLFSDEPVKCDPPTVLDALT
                     ALMERKMAYKPGERDLVVLQHEFIAEYPGGRKEAITSLLVDYGIPYGDSSMARTVSLP
                     AAVAVKLMNQGKIKLTGVHIPTVPEVYEPVLAELEKMNIKFVDTFKPLA"
     gene            complement(2230..3540)
                     /locus_tag="PKM23_01495"
     CDS             complement(2230..3540)
                     /locus_tag="PKM23_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysine ketoglutarate reductase
                     /saccharopine dehydrogenase family protein"
                     /protein_id="HNY90174.1"
                     /translation="MKKIIGIRREDKNIWERRAAIIPEQIIKFREELPLEFYCQPYPT
                     RAFSDQEYERAGARIEEDLTPCSVIFGIKEIPIPLLHPGKTYLYFSHTIKGQKYNMPM
                     LRHLLESRSTLIDYELIKDARGRRLVFFGRFAGMAGMVDTLHALGLRLRSQGFETPFT
                     DIRMAYEYRDIAEAKAAVETAGRRIAEQGIPEPLRPLVIGFSGYGHVSKGAQEVFDLL
                     PHQEVEPQELAGLTPRRDTILHKVVFAERDMVKPVDPAQAFDLNDYFEHPEGYHSQFE
                     AHLPHLSVLVNGIYWDKRYPRLLTKRYLKERWSEGRPIKMQVIGDISCDINGSIECTE
                     KATESDNPIFVYHPGTDTIRDGHEGEGVVIMAVDNLPAEIPRDASQAFSQSLHPFVPA
                     ICLADYSQPFGQLNLPTEIKNAVICHQGQLTPSYSYLEEHLNKA"
     gene            complement(3709..4737)
                     /gene="rlmN"
                     /locus_tag="PKM23_01500"
     CDS             complement(3709..4737)
                     /gene="rlmN"
                     /locus_tag="PKM23_01500"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNY90175.1"
                     /translation="MKNKLVGLNRAELELFAESIGEKRFRGRQLFSWIYAKHAASFEV
                     MSDLGRELRRKLAEEAGLEWMTLEQRAASPESGSVKYLFRLEDGALIEAVYIPESGRR
                     TLCISSQVGCALGCAFCATGALGFRRNLTTGEIADQVLQAARETGEMPTNIVFMGMGE
                     PFLNYEAVTAAAGLINNPDGIAVGHRHIVISTVGITPAILRFADEGQPFRLAVSLHSA
                     IDAKRRQIVPIAKRYPLPELLNAVKYYAKKARRRPTFEYVLLAGFNDGEEDADALRRL
                     AAEVPCKVNLIPYNPALPGFERPDEASVTRFATWLSPLHAPVSIRWSKGTDINAACGQ
                     LAGREGRI"
     gene            4973..5971
                     /locus_tag="PKM23_01505"
     CDS             4973..5971
                     /locus_tag="PKM23_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNY90176.1"
                     /translation="MELRSARDLDAVQQLKQAHQAITGEIGKVIIGQHQVIDEMLISL
                     LSRGHSLLVGVPGLAKTLLISTLARVLDLQFKRIQFTPDLMPSDITGTEVIEGETGGG
                     RKAFKYVKGPIFANIVLADEINRTPPKTQSALLQAMQEHEVSAAGVTYKLDEPFFVLA
                     TQNPIEQEGTYPLPEAQLDRFMFNLWVDYPSEAEEVEIVTTTTSAYHADLKVILKAAD
                     IIALQDLVRRVPVAENVISYAVQLARKTRPGHDSAPKFIKEWITWGAGPRASQYLILG
                     AKTRAILDGRPTPDIADVRALAKPVLRHRLVANFNAEAEGVTTSQIIDRLIAEQEG"
     gene            5975..6883
                     /locus_tag="PKM23_01510"
     CDS             5975..6883
                     /locus_tag="PKM23_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNY90177.1"
                     /translation="MASQAPDYRQFLQPAVVSKLTNLDLIARLVVEGFITGLHRSPYH
                     GFSVEFAEHRPYMPGDDIRHIDWKVYAKTNRYYLKEFEEETNLKAHLLLDASASMGYG
                     SGAVTKLQYAIWLAAALAYLMIHQRDAVGLVTFDEKIRRYLPPRSIFSYLTLLLQEMQ
                     NTASSAATDVAQTLHQMAERIHRRGLVILFSDLLDDPQKIISGLKHFRHNNHEVIVFH
                     LLDPLELSFNFRQDALFIDLESGDRMTIQPWHIRGEYHRLMTEHIETLRRQCRENRID
                     YLLIDTSQPFDTALLEYLGKRKRIGG"
     gene            complement(6946..7362)
                     /locus_tag="PKM23_01515"
     CDS             complement(6946..7362)
                     /locus_tag="PKM23_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90178.1"
                     /translation="MTKRSFLYIAFALLGLGSLLHAGAIISSFQGETGYNQVELRWIV
                     AAENNLKGYQVLRSLDSRDYETLVTIGVKDKGEGERSYSYIDKSVFKQTTHEFYYKLR
                     MVNEDGSFTDYDKVLRLSPQISSARQTWGSIKAMFR"
     gene            7582..8442
                     /locus_tag="PKM23_01520"
     CDS             7582..8442
                     /locus_tag="PKM23_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNY90179.1"
                     /translation="MAGTGILFQTANIEDSLDRYLREINEIPLLQASEEIDLAKRIRA
                     GDQDALEMLTKSNLRFVVSVAKQYQNQGLSLGDLINEGNLGLLKAATRFDESRGFKFI
                     SYAVWWIRQSILQALAEQSRIVRIPLNRIGALNKIEKLFSSLEQEYEREPTKEELAEE
                     MDMSPADISDLMSNTSKQLSLDAPIDEEEENKLMDVIEDYEMPAPDFQLQAESLRIEI
                     ERALASLEAREAEVIRLYFGLGMEHPLTLEEIGQRFTLTRERVRQIKEKALRRLRHNS
                     RSNLLRKYLG"
     gene            8621..9544
                     /locus_tag="PKM23_01525"
     CDS             8621..9544
                     /locus_tag="PKM23_01525"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNY90180.1"
                     /translation="MRNKQMKLLYFSLGGTELKEISLGWKKIVGIIVLGIAAIVLLVS
                     VSVGLITALVNDYQVANLSRSNNRLQSLLADMSKEVQVIESKLKSVEQQDDDLRVFVD
                     MPTIDSDIKKLGTGGKVNASAIDLAYGSGNVNQQAYQMKDLLDNLSQRAELAVESRQE
                     IMKEYYDNLRRLKQTPSIRPLGGGRVTDKFGYRLDPFIDRFKHHTGVDFSAPRGTEVY
                     ASADGKVVETVNDYRPNYDYGKYVLINHGFGRLTRYAHLDQISVKPGQVVNRYTVIGR
                     VGDTGRSTGPHLHYEVIVDGKPVDPFAYILD"
     gene            9604..10620
                     /locus_tag="PKM23_01530"
     CDS             9604..10620
                     /locus_tag="PKM23_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY90181.1"
                     /translation="MPTPINRSIRPLLLALLLLAAHAPLSGQPPAALLRQGVELGVIH
                     DYEPALDLFGRLSTEYPGRPEGPFYRAAIHQSMMLDFESGEWQELFYADLEEAIHRAL
                     QQLRLQPEDAEARFYAGAAYAYKSAQLSRDGKYWPAYRAIQSSLDYLEPLLKEDSTWC
                     DALLGVGTYQYWRSRITIKLSWLPFFPDRRREGIAMITRAAACAQLSPASAWSNLAWI
                     SIREKEYDRAIVWSRTGLERYPGSRFFLWPIAEAQFLKGDYSAAIGSYSNLLLSTREA
                     PYNNGYNELVILWKMAQCHERIGENDLARSYYQEMVDCRVAEEVRGRAESKIRMAREW
                     LAAH"
     gene            complement(10617..11078)
                     /locus_tag="PKM23_01535"
     CDS             complement(10617..11078)
                     /locus_tag="PKM23_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90182.1"
                     /translation="MPDLVTHGFAAWLLVRPQRWRRVRLLFFLGTFLPDIISRPIYIL
                     FPHWQGYTVAMHTPLFALLCGLLAAEFMTPDLRRCARLAISAGILLHFGLDLMQSHLG
                     DGYYWFFPFSWRSYELGWFWPEATVVWVPLWLAIITTAEAVLAWRGRHSAA"
     gene            complement(11063..11509)
                     /locus_tag="PKM23_01540"
     CDS             complement(11063..11509)
                     /locus_tag="PKM23_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90183.1"
                     /translation="MQRPLRITLLTLVLAALIWYGHSANPAVTLRDCLADPGRYEGRR
                     ITLATEVTVVQTWPDSLIVHQAGLKVKVIGPPGDARPGEYVSLIARFRSPGRLELEAL
                     HPARGRRAKIVWSILPLLLLAFFWLAAYRFDCRRFYWEERQRCRTS"
     gene            complement(11513..12319)
                     /locus_tag="PKM23_01545"
     CDS             complement(11513..12319)
                     /locus_tag="PKM23_01545"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNY90184.1"
                     /translation="MHLVREGEEAAAAARAAGRLLMDHFGTIEQKDVDRKGAADFVTF
                     VDKSSERLIIERLGAAFPDHRFYAEESSRSEAGGWRWIIDPLDGTTNYIHGIPVFSIA
                     LALEHEGEILFGLVFDPVHDELFTAEKGHGARCNGEPIGVSLIRDPSLALLATGFPFK
                     QKHNLGRFLASFEEIFLQVSGIRRMGSAALDLCHVACGRYEGFWELGLAPWDVAAASL
                     IITEAGGRISDFRGGDEAIWGGNVVASNGHLHELLLAAARRHLADLMTAE"
     gene            complement(12363..14741)
                     /locus_tag="PKM23_01550"
     CDS             complement(12363..14741)
                     /locus_tag="PKM23_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNY90185.1"
                     /translation="MISTFQTLEFDRVREKLAECASSELGHERALVVEPLSDENLLAE
                     RMAQTTEMRDLLDYDQALPIDGIHDLRPQVRSLRIGGSYLSPEELMPVLRTLAAVRRL
                     RLYFVSRSEKMPCLTRIAEQLEPREAVEKEIRRCIDENTLVVKDEASPELAGVRKQLA
                     RAQSHARQRMESIQRTLAGQGVLQENLITVRNGRLVLMVKEEYKRKVRGLVLDQSASG
                     SSLFVEPIESVEDNNRIRELEAEEAREVERILRHLADLLRAALPAIEANLELLARLDL
                     LYAQGCFSRRLNAHPPELVPEPLLHIASGRHPLLVLRMGEKEVIPLDILLGENSHTII
                     ISGPNAGGKTVALKTVGLLTLMARSGLHIPALPHSRIGAIKRVYASIGDQQSIDNDLS
                     TFSSHLAGLKAIAESAGAGSLVLIDEIGAGTDPEEGSALAMAILEKLTDRRALSLVTT
                     HQSALKAFAYRTDGVENGSMAFDIDTLQPTYHFRAGIPGSSYAFEIAGRMGLAAGLIS
                     RAQELVGAQKDRLEGLILELEGKIQHYDEIGRQADLRETEYRGLLKLYQERSEQLRRE
                     EKALKRKAAEEAEEILRRTNSTVESAIREIREKQAQTEAIRAAKQALESRRREVAQEL
                     EKVKAVEPPPAEESAESGPIQVGDAVRWEKMSSVGEVVTEPDQQGRVLIQVEGVKIRA
                     PLAELRKSSRRELRRRGGTRILLDEEAPVRNEVDLRGLRAEEAIERVEVYLDEAIMAG
                     YSEVRIIHGKGTGALRASIGRFLRDHPRVANARLGNWNEGNAGVTVVELRPD"
     gene            complement(14738..15274)
                     /locus_tag="PKM23_01555"
     CDS             complement(14738..15274)
                     /locus_tag="PKM23_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNY90186.1"
                     /translation="MSPFDLIIIAALVWFVYTGAKIGLAKELVGLTGWLIAALVALKL
                     GGRAGAMATGFLPSLKALPVELTGFLIALFGTHIFFRVIGYLFNNMLGGNGQSTLDRM
                     GGAFIGFVKGAFIISVVALALNSLYLGDKLESYQNNSSLFPHMSKFAQMVVNQVIRAT
                     PSAGVREATRPAEDYPEE"
     gene            complement(15372..15818)
                     /locus_tag="PKM23_01560"
     CDS             complement(15372..15818)
                     /locus_tag="PKM23_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNY90187.1"
                     /translation="MSLLDRLTEDMKSAMKAGEAVRLGTIRLLRGQIKDAAIDKRAEL
                     TDDEMLGVLANAAKKRREAIEAYRNAGRVDLAEKEEAELAVIQGYLPAALDTAEIETI
                     IDRAIAEAGAQTIRDLGKVMPLVMNETKGRADGKMVSEMVRRKLSL"
     gene            complement(15856..16050)
                     /gene="rpsU"
                     /locus_tag="PKM23_01565"
     CDS             complement(15856..16050)
                     /gene="rpsU"
                     /locus_tag="PKM23_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNY90188.1"
                     /translation="MPGVRIRENEPFERALKRFTKACEKAGLMSDIKKHQHFEKPSER
                     RKRKLNAARRKMRKLQMMER"
     gene            16273..16608
                     /locus_tag="PKM23_01570"
     CDS             16273..16608
                     /locus_tag="PKM23_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90189.1"
                     /translation="MLKKILLAILILLIAGLAYLYLNKDKIARVAIEKSLPLIETSLL
                     ENLPGDVNRDDVKAVFDRIDVKVKEGKVDIMQMQTLLENFQQALKDQKVDEEEFHKVY
                     AEIKKLAGD"
     gene            16716..17333
                     /gene="nrfH"
                     /locus_tag="PKM23_01575"
     CDS             16716..17333
                     /gene="nrfH"
                     /locus_tag="PKM23_01575"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNY90190.1"
                     /translation="MKLNLRHLIRQISPPPKWRIPVLFLLALLTGLGGLTLHLARATS
                     YFSDDPLTCLNCHVMRSQFATWQRSSHTRVATCNDCHVPHDSFIRHYAFKAQDGLRHA
                     FMFTFRLEPQVIRIKSAGAGVVRENCIRCHRQLVEQLPMYKFAAASPRHRVPDKSCVE
                     CHAGTPHGRVNSLASVPMVRVPRLTPAAPAWLMQPRNRAAVDPEP"
     gene            17351..18802
                     /gene="nrfA"
                     /locus_tag="PKM23_01580"
     CDS             17351..18802
                     /gene="nrfA"
                     /locus_tag="PKM23_01580"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HNY90191.1"
                     /translation="MSGDQTKFTQKPLFYWMLFIATAVVVFLIGLFGASVLERRSESY
                     QLLQPVKPIADWEPSNAAWGENYPREYETYRATAETTFASLHGGSATIDALERYPRLV
                     VLWAGYAFSRDYKQGRGHYHAVEDIRNTLRTNVPQPGTCWSCKSTDVPRLINKMGAAE
                     FYKAKWEDLGSEVVNNIGCQDCHDPKTMNLRITRPALIEAYQRQGKKIEESTHQQMRS
                     QVCAQCHVEYYFKGKQEKYLTFPWDEGSTAEQIQAYYDKVEHVDWVHKISGAPMLKAQ
                     HPDWEIFQMGIHFQRGLACADCHMPYRSEGGVKFTNHKIASPLENVAGSCQVCHRESE
                     KALIDNVQERQEKVRQLAGMAEDLLVRAHIETRVALDRGATPEQIKPVYQLIRQAQWR
                     WDFVVASHGGSFHAPIECARIMGNAIEKAAEARTQLAGLLGRLGVELPVAMPDISTKA
                     KAQRYIGLDMARLEAEKADFKARVISTWGEPAL"
     gene            18820..19794
                     /locus_tag="PKM23_01585"
     CDS             18820..19794
                     /locus_tag="PKM23_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNY90192.1"
                     /translation="MAEQILEFIVAPAQGKERLDLYLASRLPAVTRAKLKRLIDDGAV
                     SLDGRPTKAGHLVRPGETIVVTFPAAAPKGVEPEEIPLDILYEDEHLLVVNKAAGMVV
                     HPAYANLSGTLVNALLAHCRSLSSIGGDARPGLVHRLDKETSGLLVVAKNDLTHVGLA
                     RQLSEHKMEREYRAIVWGHLARKQGCVRAALMRNPKDRLRMMIHPEGKMAVTHYQVLE
                     ELPLTSYMQLNLETGRTHQIRVHMSSLGHPVFADATYGGRAKQLAGLNHENTRLGLHL
                     LKSCNRQMLHARTLAFTHPVTGELQRFECAIPADMQALLEELRSQSEV"
     gene            19859..20713
                     /locus_tag="PKM23_01590"
     CDS             19859..20713
                     /locus_tag="PKM23_01590"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HNY90193.1"
                     /translation="MKIVPLLALLLCALTAGAEAEIVSMEKKYHIRGDMELDMRVDGG
                     KVEILRSEDNDCHILIEYDRERCRADVNLDEDTDVLELFVDHDNWAMTKKSVDDKSKY
                     ARIRISLPPKADLALNLEMKAGELDMKVGDLHIKSFELRSYAGKTSIDFDQPNRCEMK
                     TFDVDFKIGEVKLYNLGNANFAEADINSSVGSMLIDFNGEKIEQAMARIDLEIGESTI
                     VVPEKIGVKIKVSKFLFLSNVDYPNWFTQEGSYYYSRNYNKKKESLYLVVSTGIGELK
                     IRVDKKND"
     gene            20878..23580
                     /locus_tag="PKM23_01595"
     CDS             20878..23580
                     /locus_tag="PKM23_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY90194.1"
                     /translation="MILILVLLLLAFPLYGQGDLYLVFGSDTSIWDGMSTTNFVSHYR
                     PDTYSAPASNAAVVIDEGFRARLRDSEGRPMPFTWWMHSGNLFRHADNCDVPLANTIA
                     IHLMKRYHGDRIARVGDELTLHYHTWDWTDENGDGIYCWNQTRSFEQCREDFDLTLCH
                     YLLEEEIFPVSFRSGWHYMDNIWQAYLDELLPFSLHSAYPSKKLTSEEPTNNIYDWSQ
                     ATSRWVPYKPSQDNYQLPGGSRGWNVRCIYMGGLKAAAVEGMFAGANAGVDQLACIWS
                     HLPEADFPEQITKAHTLIVEAAKRYPKVRFHYMTAIDGYQAWLRTADRQAPTLTFRDN
                     GPAEQPVFSIETDEPIFQRQPFVAIKDIYSRYQILPCTRTGAFTWEAVSPLGRDRLVK
                     AGVALCDTVGNQALQLIRYLPEDIFLDNESGAYREQTPLFRTIDDYAWGTNARAATIA
                     GGDSAVVRFELPVREKRAYQLLCQFAPTAHPADTLTAILTQSGRIIWREEITGPKAAK
                     RWHFVTVSELDPAGAPALYMIAHNRSAELREFSLDALRITPIIPDRKLEVSPALFHFG
                     ETSLYTRVEKNLLIHNAGSGPLSIHSISTRMGLFQLSTAGPLTIPPFSSDTLAVAFES
                     DHAVMATDTLVIASDDPVQPRHVLPLTVEASAFFITADNDDPVSYFEYGVWFNSSVEA
                     SGASSRYISLSGHLGAWAQFMATPPRSGTYAISIILPKTENATNHALYKILARGQTLD
                     SLYIDQNAGSGGWVELGRYDLMAGRPVLVQVIHAGGQTAGAVLRADAVRFILQGESTG
                     LARTESGVLPGEVRLLPNYPNPFNASTTICYSLPAAAGVHLEIYDLQGRRVRTLSREI
                     RMAGEHTVTWDGCDEAGRVVASGLYFYKLSGEDFSLSRRMFLVR"
     gene            23604..23888
                     /locus_tag="PKM23_01600"
     CDS             23604..23888
                     /locus_tag="PKM23_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90195.1"
                     /translation="MKRIHHLLLLLMLAALLLLAGCAPGTGRYTAAGPAGFLDGIWHG
                     WIAPVTLIVGLFNDGVRIYEPFNKGWWYDLGFYIAVISGFGGLSLTRRKK"
     gene            complement(24102..24656)
                     /locus_tag="PKM23_01605"
     CDS             complement(24102..24656)
                     /locus_tag="PKM23_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HNY90196.1"
                     /translation="MKKSLWMVGLVFVLALVFSMNLFAQTEKAQYIGAAKCKMCHTAK
                     AKGEAHPIWSKSKHASAYATLGTEEAKAVGKKAGIDDPQKSSKCLKCHVTAYDAPATA
                     KAATYKMEDGVTCETCHGPGSLYQSMKVMKALAAGTQDHAAVSYNPGDLKSCVKCHNQ
                     ESPTYKPFKAEQDWKQIAHPTPKG"
     gene            complement(24801..>25332)
                     /locus_tag="PKM23_01610"
     CDS             complement(24801..>25332)
                     /locus_tag="PKM23_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY90197.1"
                     /translation="ATNRDLEEAVRSNTFREDLYYRVNVFPIMLPPLRDRVEDIPALA
                     DHFLAKQNSQTAGIDAGALHSLMEYNWPGNVRELENIIERALIMSGGKQITAEDLPVH
                     LRHSGENAPAPARGVGEIPTLEEMERRLIDKALEKAEGNKSLAARLLGITRRQLYSRM
                     ERWGEGGEDENNEPEE"
BASE COUNT         5137 a         7900 c         7352 g         4943 t
ORIGIN      
        1 gatgccgatg aagccgccca gaatggaaaa atagagcagg taggaggaga gatagtcgcg
       61 cggctggagc agaatccaga ccggggtgat ggaggcgacg aaggcgtaga gaatgagcgc
      121 gatgctccag aaccggccgg cgcttaaatc tgaaaaggag gcgacgcccg ccgcatcgat
      181 gggtatgatg tggccgatcc aggtggccag taaaaccagc ggcacgaaga tgagcgagct
      241 ggcgagcagg gaaagtttaa gccggtagag ggagaggcca aagcccatcg ccaggagcat
      301 aaagatcagc gaggaggtgg ccaccccgcc gtcctcgacg aaagtggtgg cggtgaggtc
      361 ggtgaagacg gtgaggacat agaccagggt cagccagatg aaagcgagaa agagcttgta
      421 ggcgaggggg ctcatctgct cacgggcgat ctcggcgatc gagcgcgcct tgtggcggat
      481 ggaggcgacc agggcggtgt aatcgtggac cccgccgatg aaaatcgaac cgaggatgat
      541 ccagagcagg gtcggcagcc agccgaaggc agcggtggcg atgatcgggc cgacgatcgg
      601 tcccgccccg gcgatcgagg aaaaatggtg gccgagcagc acctgtttcg gggcgggcac
      661 gcggtcgatg ccgtcatagt cggcatggct cggagtcgga ttggcattgt caatcgagaa
      721 ctgccggtcc agaaaggcgc cataaaaacg ccaggccagc aaaaacagca gcacggcggc
      781 gaggaggatg gtgaacagca tggcctcttc cctcattcat aacggcctgc acgcgcaggg
      841 ggtggtatga aaagagcccg gatgatccgg gctcttttcg gttgtggatg ctcaagccag
      901 gggcttgaag gtatcgacaa acttgatatt catcttttca agctccgcca ggaccggctc
      961 gtaaacctcc ggcacagtgg ggatgtggac gccggtgagc ttgatcttgc cctggttcat
     1021 cagcttgacc gcgaccgccg ccggcaggct gacggtgcgc gccatggagc tgtcgccgta
     1081 ggggatgccg taatcgacga gcaggctggt gatcgcttcc ttgcggccgc ccgggtactc
     1141 ggcgatgaat tcgtgctgca gcacgaccag atcgcgctcg cccggtttgt aggccatctt
     1201 tctctccatc agggcggtga gggcatcaag gaccgtgggc gggtcgcact tgacgggctc
     1261 atcgctgaaa aggcccagcc actcgaaccg cttgatcaca tcatggacct ccggcagctc
     1321 caggtgtttg gccatagcgg ccttgagccc ctcgggcttg gcattgatca gggaggcggt
     1381 cagttgcgcc caggtccagc cgagaatctc cggccgttcg gtgtcattca gaaagccgag
     1441 gcgcaccacc ttctccaggg tttcgcacca gccgggattg cggaaggtac cgcggaagat
     1501 agtctgggca tccttaagcc cgtagagatc ctgatagagc atggaatcgc ggttgggata
     1561 gagttcgagc cagcccagtc cccggacatc cttgatcgcg acggtacgga agaggtcctt
     1621 gttctcgatg accatatcct tgccgtcctt gagaaaatgg gccgaattgc gtccggcgag
     1681 gaggacgccg cggggactcc aggagaactt gtagccgaag ggattgtcgt tggcttcggg
     1741 ggcgggcagt ccaccgcaga ccgattcaaa ggaggtgacc cttcccccct tgtgctccac
     1801 ctcatggatc atcttcatcg ccgacatgtg gtcgatgccg gggtcgaggc cgatctcgtt
     1861 gaggaacaat agtccctggt ccctggcctg ctggtcgagg gcgcgcatgg ccgggctaac
     1921 atagctggtg gtcaccagat tggtcttgtg cttgaggcac aattccgcca cctgcacatg
     1981 atagatccac ggcagcaggc tgatggccac atcggcatcc gccaccaggg cctcgagctt
     2041 ttccttctcc tgcaccgtgg ctgcgaccgc cttgcccgca ggatgcttct cgatcagggc
     2101 ctccgccttg ctgaccgtgc ggctggcgat gatcacctgc gtatccggct gatcgagcag
     2161 atagcgcacc aggggacgtg aaaccagacc agcaccaaga accagtactt ttttcatacc
     2221 tcttctcctt caggctttgt tcaagtgttc ttcaagatag ctgtacgacg gcgtcaattg
     2281 gccctgatgg cagatcacgg cgttcttgat ctccgtcggc aaattcagtt ggccaaaggg
     2341 ctggctgtaa tcagccagac agatggccgg aacgaaggga tggagcgact ggctgaaggc
     2401 ctgcgatgcg tcgcggggga tctccgcagg cagattgtct accgccatga tcaccactcc
     2461 ttcaccctca tggccatccc ggatggtatc ggtgccgggg tggtagacga aaatgggatt
     2521 gtccgattcg gtcgcctttt cagtgcactc gatcgaaccg ttgatgtcgc agctgatatc
     2581 cccaatcacc tgcattttta tcggccgtcc ctcggaccag cgctctttca gatagcgctt
     2641 ggtcaggagg cgggggtagc gcttgtccca gtagatgccg ttgaccagga cgctcaggtg
     2701 gggaagatgc gcctcaaatt gggagtgata gccttcggga tgttcaaaat agtcgttcag
     2761 atcgaaggcc tgcgcgggat cgaccggctt gaccatgtcg cgttctgcaa agaccacctt
     2821 gtgcaggatg gtgtcgcgcc gcggcgtcag cccggccagc tcttgcggtt cgacctcctg
     2881 gtgcgggagc aggtcaaaga cctcctgagc tcccttggag acatggccat agccggaaaa
     2941 accgatgacg agggggcgaa gcggttcggg aatcccctgt tccgcgatgc ggcggcccgc
     3001 tgtttcgacc gcggccttgg cctcggcgat atcgcggtat tcgtaggcca tgcggatatc
     3061 ggtgaagggg gtctcaaaac cctggctgcg caagcgcagg ccgagggcat gcagggtatc
     3121 caccatgccc gccatgccgg cgaagcggcc gaagaagacc agtcggcgcc cccgggcgtc
     3181 cttgatcaac tcataatcga tgagggtgga gcggctctcg agcagatgcc gcagcatggg
     3241 catgttgtat ttctggccct tgatggtatg cgaaaagtag aggtaggtct tgccgggatg
     3301 aagaagcggg atcggaattt ccttgatgcc gaagatgacg gaacagggag tgaggtcctc
     3361 ctcgatgcgg gcgccggccc gctcatactc ctggtccgaa aaggcgcgcg tcgggtaggg
     3421 ctggcagtag aactcgaggg gcagctcctc cctgaatttg ataatctgtt cggggatgat
     3481 ggctgctctg cgctcccaga tgtttttatc ctcgcgacgg attccgatga tttttttcat
     3541 ccctctcctc tgtgcctgtt cctgatcggg tggccccgcg gtgcctttcc catgccgggc
     3601 ggcggttcga aacaatggtc ccctattcta gcaaaaatag cccagaatgt caacgtattt
     3661 ttaaagcaga ccagggtaat ccggggacaa agaacacccc tgctgctctc atattctccc
     3721 ctcccttccc gcgagctgtc cgcaggcggc gttgatgtcg gttcccttgc tccagcggat
     3781 cgagaccggg gcatgcaggg ggctcagcca ggtggcaaag cgcgtcacgc tcgcttcgtc
     3841 aggacgctca aagccgggca gggccggatt gtagggaatg aggttgacct tgcacggcac
     3901 ctcagccgcc agcctgcgca gggcgtcggc atcctcctcg ccgtcattaa agcccgcgag
     3961 gaggacatac tcgaaggtcg gccggcggcg ggcctttttc gcataatact tgacagcatt
     4021 gaggagttcg ggcagggggt agcgtttggc gatggggacg atctgccggc gcttggcatc
     4081 gatggccgag tggagtgaaa cggcgaggcg gaagggctgc ccctcatccg cgaagcgcag
     4141 gatggccgga gtgatgccga cggtggagat gacgatgtga cggtggccga cggctatgcc
     4201 gtcggggttg ttgatgaggc cggcggcggc ggtgacggct tcatagttga gaaagggctc
     4261 acccatgccc ataaagacga tgttggtcgg catttccccg gtctcacgcg ccgcctgcag
     4321 cacctggtcg gcgatctcgc ccgtggtgag gttgcgcctg aaacccaggg cgccggtggc
     4381 gcaaaaggca cagcccaaag cgcagccgac ctgggaagag atgcagaggg tccggcggcc
     4441 ggactcgggg atatagaccg cctcgatgag cgctccgtcc tccagccgga agagatattt
     4501 gaccgaaccg ctttcgggcg aggccgcccg ctgctcgagg gtcatccact ccaggccggc
     4561 ctcctcggcc agtttgcgcc gcagctcgcg gccgaggtcg ctcatcacct cgaaagaggc
     4621 ggcgtgtttg gcgtagatcc agctgaagag ctgccggccg cggaatcttt tttcgccgat
     4681 cgattcggca aagagctcca gttcggcgcg gttcaaaccg acaagtttat ttttcataga
     4741 ccattcacaa aagggcacgg gctgtgcgcg gcccgcacgg ccgcggatcg gtgatcgggg
     4801 agggatccgc gccccctaaa ttagcagatg tttttctatt ttccaatctt ttccgcaatg
     4861 cggaacaaca gctggtactt gccggttata actagacagc ggcgcgtgtt gtctttttcc
     4921 gccggtatgt aaaagttgaa taaatactgg aatcgatgga tagggaggcg gcatggaatt
     4981 acgcagcgcc cgcgatctgg atgctgtgca gcagctcaaa caggcccacc aggccatcac
     5041 tggcgagatc ggcaaggtca tcatcggtca gcatcaggtg atcgacgaga tgctcatctc
     5101 cctgctttcc cggggtcact ccctgctggt cggggtcccc ggtctggcca agaccctgct
     5161 catcagcacc ctggcgaggg ttctcgatct gcagttcaaa cgcatccagt tcaccccgga
     5221 cctgatgcct tcggacatca ccggcaccga ggtgatcgag ggggaaaccg gcggcggccg
     5281 caaggccttc aagtatgtca aggggccgat cttcgccaac attgtcctcg ccgacgagat
     5341 caaccgcacc ccgccgaaaa cccagtccgc cctgctgcag gcgatgcagg agcatgaggt
     5401 ctccgccgcc ggggtcacct acaaacttga cgaacccttt ttcgtcctgg ccacccagaa
     5461 ccccatcgag caggagggca cctatcccct gcccgaggcc cagctcgacc gcttcatgtt
     5521 caatctctgg gtggactatc cgagcgaagc cgaagaggtt gagatagtga ccaccaccac
     5581 cagcgcctac catgccgacc tcaaggtgat cctcaaggcg gccgacatca tcgccctgca
     5641 ggatctggtg cgccgggtgc cggtggccga gaatgtgatc tcctacgccg tccagctggc
     5701 gcgcaagacc cgcccgggcc atgacagcgc gcctaaattc atcaaggagt ggatcacctg
     5761 gggggcggga ccgcgcgcct cgcagtacct catcctcggg gccaagaccc gcgccattct
     5821 cgacggccgg ccgactcccg atattgccga tgtacgcgcc ctcgccaaac cggtgctgcg
     5881 ccatcgcctc gtcgccaact ttaacgccga ggccgagggg gtcaccacca gccagattat
     5941 cgaccgtctc attgcggagc aggaggggta atccatggcc tcgcaggcgc cggactacag
     6001 gcagttcctc cagcccgcgg tggtctccaa actgaccaac cttgacctca tcgcccgcct
     6061 ggtggtggag ggctttatca ccgggcttca ccgctccccc taccacggct ttagcgtcga
     6121 gtttgccgaa caccgcccct acatgccggg ggatgatatc cgccatattg actggaaggt
     6181 ctatgccaag accaaccgtt attacctcaa ggagttcgag gaggagacca acctcaaagc
     6241 tcaccttctg ctcgacgcct cggcatcgat ggggtacggc tcgggcgcgg tgaccaaact
     6301 gcagtacgcc atctggctgg cggcagccct ggcctacctc atgatccacc agcgtgacgc
     6361 cgtcggactg gtcaccttcg acgaaaagat ccgccgctac ctgccgccgc gctcgatttt
     6421 cagctatctg accctgctgc tccaggaaat gcaaaacacc gccagctcgg cggcaaccga
     6481 tgtggcgcaa accctccacc agatggccga acgcatccac cgccgcggac tggtgatcct
     6541 cttctccgac ctcctcgacg atccccaaaa aatcatttcc ggcctcaaac acttccgcca
     6601 caacaaccac gaggtgatcg tcttccacct ccttgatccc ctcgaactct ccttcaactt
     6661 caggcaggac gccctcttca tcgaccttga aagcggcgac aggatgacaa tccagccctg
     6721 gcacatccgc ggcgagtacc accggctgat gaccgaacac atcgaaaccc tcagacgcca
     6781 atgccgcgag aaccgcatcg attatctcct gatcgatacc tcccagccct tcgacaccgc
     6841 cttgctcgag tatctcggca agcgcaaacg catcggggga tgacggccgc agccgcgggc
     6901 aaaaaaaagg cccggaatcc gggcctggaa aagagtgcgg ggtgatcagc ggaacatcgc
     6961 cttgatgctg ccccaggtct gccgcgctga ggagatctgc ggcgaaaggc ggagcacctt
     7021 gtcataatcc gtaaagctgc cgtcctcatt gaccatgcgc agcttgtaat agaactcgtg
     7081 ggtggtctgc ttgaagacgc tcttgtcgat gtagctgtag ctcctctccc cttccccctt
     7141 gtccttcacc ccgattgtga ccagggtttc ataatcacgg ctgtccaggc tgcgcaaaac
     7201 ctggtagccc ttgaggttgt tctccgcggc gacgatccac cgcagctcga cctgattata
     7261 gccagtctcg ccctgaaagg aggagatgat ggcgccggca tgcagcagcg atccgagtcc
     7321 cagcagggcg aatgcgatgt ataaaaatga acgtttggtc atgatgcggc ctttattatc
     7381 gataccccgg aatattctag taactaatat acaaagattt ccgtcaaagt caattgtttt
     7441 ctgcagagga tgcaaattaa acctatttat gtatataaaa atgaacttgc gtaaatcaga
     7501 tgttgacaaa aagaactgct ttcagtatat tagcccgaca ggggcaagct gttccgatgc
     7561 taacaaccag gggaaaatag attggcaggg accgggatac tattccagac cgcaaatatc
     7621 gaagactccc tcgaccggta tttgcgggag atcaatgaga ttccgctgct ccaggccagt
     7681 gaggagatag acctcgccaa acgcatccgg gccggggatc aggatgcgct ggagatgctc
     7741 accaagtcca atctccgctt cgtcgtcagc gttgccaaac agtaccagaa tcagggcctc
     7801 tccctcggcg acctcatcaa cgagggcaat ctgggcttgc tcaaggcggc cacccgtttt
     7861 gatgagagcc ggggcttcaa atttatctcc tatgccgtct ggtggatccg ccagtccatc
     7921 ctccaggccc tcgccgagca atcccgcatc gtccgcatcc ctctcaaccg catcggcgcc
     7981 ctcaacaaga tcgaaaagct cttttcgtcg ctcgagcagg aatacgaacg cgagccgacc
     8041 aaggaagaac tggccgaaga gatggatatg agtccggctg atatctcgga tctgatgagc
     8101 aacaccagca agcagctctc actcgacgcc cccatcgatg aagaggaaga gaacaagctg
     8161 atggatgtca tcgaggacta tgagatgccg gcgcctgatt tccagctgca ggccgaatcc
     8221 ctgcgcatcg agatcgaacg cgccctggct tcgctggaag cccgcgaggc cgaggtcatc
     8281 cgcctttact tcggactcgg catggagcat cccctcaccc tcgaggagat cggccagcgc
     8341 ttcaccctca cgcgcgaacg ggtccgccag atcaaggaaa aagccctgcg ccgcctgcgc
     8401 cataactccc gcagcaacct gctcagaaaa tatcttggct gacaaacatt atattttaca
     8461 ttgacttaca tttttgatag taattttttc ttgacaaatt tccgaaaggt tagtaaatta
     8521 gtgcattagc cctccacatt catcccccat gtattgacaa acaaagtgaa gtggcgtcca
     8581 aggacgggag aacctatcat taaactcaaa ggaaatagaa atgcgcaaca aacagatgaa
     8641 gttgctctat ttctctttgg ggggtacaga acttaaggaa atcagcctcg gctggaaaaa
     8701 aattgtcggt ataattgtgt tgggtattgc tgcgatcgtg ctgctggtct cggtaagcgt
     8761 aggattgatt actgctcttg tcaacgatta tcaggtggcc aacttgagcc gcagcaacaa
     8821 ccggctgcaa tccttgctcg cggacatgag caaagaggtt caggtcatcg agtccaaact
     8881 caaatccgtt gaacagcagg atgatgactt acgcgtgttt gttgacatgc ccaccatcga
     8941 cagcgatatc aaaaagctgg gaaccggggg caaagtcaac gcatccgcga tcgatcttgc
     9001 ctatggatcg ggcaatgtga atcagcaggc ctatcagatg aaggacctgc tggataacct
     9061 ctcgcagcgt gcggagctgg ccgttgagag ccgccaggag atcatgaagg agtactatga
     9121 caacctgcgc aggcttaagc agactccttc aatcaggcca ttgggcggag gcagagtaac
     9181 cgacaaattt ggctatcgtt tggatccgtt catcgaccgc ttcaagcatc ataccggtgt
     9241 cgatttttct gccccccgcg gcaccgaggt gtatgccagc gcagacggca aagtggtcga
     9301 aacagtcaat gattaccgcc ccaactatga ttacggcaag tatgtgctca tcaatcatgg
     9361 cttcgggcgg ctgacccgtt atgcccatct cgatcaaatc agtgtaaagc cgggtcaggt
     9421 cgtgaaccgc tatacggtca tcggccgcgt cggcgatacc gggcgctcca ccggacccca
     9481 tctccattat gaggtgattg tggatggcaa accggtcgat ccattcgctt acattctgga
     9541 ctagtccgtt ttattcatca aaagcctgtc caatcgacag gctttttttt tgcgcggagc
     9601 cctatgccca cgcccatcaa ccgctccatt cgtccccttc ttcttgctct tctcctgctg
     9661 gctgcacacg cccctctctc cggtcaaccc ccggccgcgc tgctgcggca gggcgtggag
     9721 ttgggcgtca tccatgatta cgaacccgcc ctggatctct tcggccgcct cagcactgaa
     9781 tacccaggcc gtcctgaagg ccccttttat cgcgccgcca ttcatcaatc gatgatgctc
     9841 gactttgaga gcggcgaatg gcaggagctc ttttacgcag atctcgagga ggcgatccac
     9901 cgcgccttgc agcagctccg cctccagccg gaggatgccg aggcccgttt ctacgccggc
     9961 gccgcctatg cctacaagag cgcccagctc agccgcgacg gcaaatactg gcccgcctat
    10021 cgcgccatcc agagcagcct cgactacctg gaacccctcc tgaaagagga ctcgacctgg
    10081 tgcgacgccc tcctcggcgt tggcacctat cagtactggc gcagccgcat caccatcaag
    10141 ctctcctggc tgcccttctt cccagaccgc cggcgcgagg ggatcgccat gatcacccgg
    10201 gccgccgcct gcgcgcagct ctcgccggct tcggcctggt ccaatctcgc ctggatctcc
    10261 atccgggaga aggaatatga ccgggctatc gtctggtcgc gcacggggct tgagcgctac
    10321 cccggcagcc gattcttcct ctggccgatc gccgaagccc aatttcttaa gggtgactat
    10381 agtgcagcaa tcggcagcta ttccaacctg ctgctcagca cacgggaggc gccctataac
    10441 aacggctata acgagctggt cattctctgg aagatggccc aatgccatga gcggatcggc
    10501 gagaatgatc tggcgcggag ctattaccag gagatggtgg actgccgggt ggccgaagag
    10561 gtgagggggc gggctgaaag caagatccgg atggcacggg agtggctggc cgcccattag
    10621 gcggcagaat ggcggccgcg ccaggccagc actgcctcag ccgtcgtgat gatggccagc
    10681 cagaggggca cccacaccac cgtcgcctcg ggccagaacc atcccagttc ataactccgc
    10741 catgaaaaag ggaaaaacca gtaatagccg tcgccgagat ggctctgcat aaggtcgagg
    10801 ccgaagtgga gcagaatgcc cgcgctgatg gccaggcgcg cgcagcggcg caaatcgggg
    10861 gtcatgaatt ccgccgccag cagaccgcag agcagggcga agaggggggt gtgcatcgcc
    10921 acggtatagc cctgccagtg cggaaagagg atgtagatgg ggcgagagat gatatcgggc
    10981 aggaaggtgc caagaaagaa gaggagccgc actctccgcc agcgctgcgg ccgcaccagc
    11041 agccaggccg caaagccatg ggtcacgagg tccggcatcg ctgacgctcc tcccagtaaa
    11101 agcggcggca gtcgaagcga taggcggcca gccagaaaaa ggcaagcagc aggaggggca
    11161 ggatcgacca gacgatcttg gcgcgacgcc ccctggccgg atggagggct tccagctcca
    11221 ggcggccggg agagcggaag cgggcgatca agctgacata ctccccgggg cgggcgtcgc
    11281 cgggtggacc gatcactttc accttcagcc ccgcctggtg gacgatcagg ctgtccggcc
    11341 aggtctgcac cactgtcacc tcggtggcca gggtgatgcg ccggccttca tagcgccccg
    11401 gatcggcgag acagtcgcgc agggtgaccg ccggattggc gctgtggcca taccagatca
    11461 gggcggccag gacgagggtc agcagggtaa tgcggagggg gcgctgcatc gctcactccg
    11521 ccgtcatcag atccgcaaga tggcggcgcg cagccgccag caagagctcg tgcaggtggc
    11581 cattggaggc cacgacattg ccgccccaga tcgcctcatc gccgcccctg aaatcgctga
    11641 tccggccgcc ggcttccgtg attattaacg aggcggccgc aacatcccag ggcgcaagcc
    11701 ccagctccca gaatccctca tagcggccgc aggcgacatg gcagagatcc agcgccgccg
    11761 aacccatgcg ccggatgccg ctgacctgga gaaaaatctc ctcgaacgag gcgagaaatc
    11821 tgccgagatt atgcttctgc ttgaagggga atccggtggc cagcagcgcc agggagggat
    11881 cgcggatgag ggagacgccg ataggctcgc cattgcaccg tgcgccatgg cccttctcag
    11941 cggtgaagag ttcatcgtgg accgggtcga agaccaggcc gaacaggatc tccccctcat
    12001 gctccagggc gagggcgatc gaaaaaaccg ggatgccgtg aatatagttg gtggtgccgt
    12061 ccaggggatc gataatccag cgccagccgc ccgcctctga acggctcgac tcttccgcgt
    12121 agaaacggtg gtccggaaag gccgcgccga gccgctcgat gatcagccgt tccgaactct
    12181 tgtcgacaaa ggtgacaaaa tcggctgctc ccttgcggtc gacatccttc tgctcgatgg
    12241 tgccaaaatg gtccataagc agacgcccgg ccgcgcgcgc cgcagcggcc gcctcctccc
    12301 cctctctcac cagatgcatg gatccgacct cctgtgctgt gatcctcagc ggacggacgc
    12361 agtcagtcgg gccgcagctc gaccaccgtc acaccggcat tgccttcgtt ccagttgcca
    12421 aggcgtgcgt tggcgacgcg gggatggtcg cgcaggaagc ggccgatcga agcgcgcagg
    12481 gcgccggtcc ccttgccatg gatgatgcgc acctcggaat agccggccat gatggcctcg
    12541 tcgagataga cctccacgcg ttcgatcgct tcctcggcgc gcagcccgcg caaatccacc
    12601 tcgttgcgca ccggagcctc ctcatcgaga agaatccggg tgccccccct gcggcggagt
    12661 tccctgcggc tgctcttgcg cagctcggcc agcggggccc tgattttcac cccctcgacc
    12721 tgaatcagca ccctgccctg ctgatcgggc tctgtaacca cctccccgac actgctcatc
    12781 ttttcccagc gcacggcatc gccgacctgg atcggacccg actccgcgct ctcctccgcg
    12841 ggggggggct cgaccgcctt gaccttttcc agctcctgag cgacctcccg gcggcgggac
    12901 tccagggcct gtttggccgc acggatggcc tcggtctgcg cctgtttctc ccggatctcc
    12961 cggatcgccg attcgacggt gctgttggtc cgccggagga tctcttcggc ttcctcagca
    13021 gccttgcgct tgagggcctt ctcctcgcgc ctcagctgtt cgctgcgttc ctgatagagt
    13081 ttgagcaggc cgcggtattc ggtctcgcgc aggtcggcct ggcggccgat ctcgtcataa
    13141 tgctggatct tgccctccag ctccaggatc agcccctcca gccggtcttt ctgcgcgcca
    13201 accagctcct gagcccggct gatcaagccg gcggcgaggc ccatgcggcc ggcgatctca
    13261 aaggcgtagc tggaaccggg gatgccggcg cggaaatggt aggttggctg aagggtatcg
    13321 atatcgaagg ccatggagcc gttctccacc ccatccgtgc gataggcgaa ggctttgagc
    13381 gcactctgat gggtggtgac cagggagagg gcgcggcgat cagtgagttt ttccaggatg
    13441 gccatggcca gggctgaacc ctcctcagga tcggtgccgg cgccgatctc atcgatgagc
    13501 acgaggctgc cggccccggc gctctcggcg atggccttga gtccggccag atgggaggaa
    13561 aaagtggaga gatcgttgtc aatcgactgc tggtcgccga tgctggcgta gacccttttg
    13621 atggcgccga tgcgcgaatg gggcagggcg gggatatgca ggccgctgcg ggccatcagg
    13681 gtgagcagtc cgactgtttt gagagccacg gttttgccgc cggcgttagg gccgctgatg
    13741 atgatggtgt ggctgttttc gccgaggagg atatcgaggg ggatgacctc cttctccccc
    13801 atgcgcagca ccagcagggg gtggcggccg ctggcgatat ggagcagcgg ctccgggaca
    13861 agttcgggcg gatgggcatt gaggcggcgg gaaaagcaac cctgggcgta aagaagatcg
    13921 agccgggcga gcagttcgag attggcctcg atggcgggca gggccgcgcg cagcagatcg
    13981 gcgagatggc gcaggatccg ctccacctcc ctcgcctcct cggcctcgag ttcgcggatg
    14041 cggttgttgt cctctactga ttcgatcggt tcaacgaaaa ggcttgatcc gctcgccgac
    14101 tggtcgagca ccagtccccg caccttgcgc ttgtactcct ccttgaccat cagaaccagc
    14161 cggccgttgc gcacggtgat cagattctcc tggagcacgc cctgcccggc cagggtgcgc
    14221 tggatcgact ccatgcgctg gcgggcatga ctctgcgccc gggccagctg tttgcggacc
    14281 cccgccagtt ccggtgaagc ctcgtccttg acaacgaggg tgttttcgtc gatgcagcgg
    14341 cggatctcct tctcgacggc ttccctgggt tcgagctgtt cggcaatgcg ggtgaggcag
    14401 ggcatctttt cactgcgtga cacgaaatag agccggagcc gccggaccgc tgccagggtg
    14461 cgcagcaccg gcatgagttc ctcgggggag aggtagctgc cgccgatgcg caggctcctg
    14521 acttggggcc tgagatcatg gataccgtca atggggagag cctggtcata gtcgagcaga
    14581 tcgcgcatct cggtggtctg ggccatgcgc tcggccagga gattctcgtc actgaggggt
    14641 tcgaccacga gggcgcgctc atggcccagc tccgagctgg cgcactcggc cagcttttcg
    14701 cgcacccggt cgaattcaag ggtttgaaac gtggaaatca ttcttcagga taatcctccg
    14761 ccgggcgcgt ggcttcccga acccccgcgg agggtgttgc gcggatcacc tgattgacga
    14821 ccatttgggc gaatttggac atatgtggaa aaagggaaga gttgttttga taggattcaa
    14881 gtttgtcgcc cagatagagg ctgttgaggg ccagggcgac gacgctgatg atgaaggccc
    14941 ccttgacgaa gccgatgaag gccccgccca tgcggtcgag cgtgctttgg ccgttgccgc
    15001 cgagcatgtt gttgaaaagg tagccgatga cgcggaagaa gatatgggtg ccgaagaggg
    15061 caatgagaaa accggtcagt tcgaccggca gggccttgag tgagggcaga aagcctgtcg
    15121 ccatggcgcc ggctctgccg cccagtttga gggccaccag ggccgcgatg agccatcccg
    15181 tcaggccgac caattccttg gccaggccga ttttggcccc tgtatagacg aaccagacca
    15241 gggcggcgat gatgatcaag tcgaaagggc tcattttctc atcctgattt actgcgccat
    15301 aaaaaaaagt gaataaagcg cccggggttg cgacgctcta ttcactcaat tccgttgcag
    15361 aaaaccgatc gtcacagact cagtttacgg cgcaccatct cgctgaccat tttgccgtcc
    15421 gccctgccct tggtctcatt catgaccagg ggcatcacct tgccgagatc cctgatggtc
    15481 tgggcccccg cttccgcgat ggcccggtca atgatcgttt cgatttcggc ggtatcgagt
    15541 gcagcgggga gatagccttg aatcaccgcc agctccgcct cctctttttc ggcgaggtcc
    15601 acgcggccgg cattccggta cgcttcgatg gcttcacggc gttttttggc ggcattggcg
    15661 aggacgccga gcatctcgtc atcggtcagc tcggcccgct tgtcgatggc ggcgtccttg
    15721 atctgaccgc gcagcaggcg aatcgtgccc agacggaccg cttcgcccgc cttcatggcg
    15781 gatttcatat cctctgtgag ccggtctaaa agactcatga tcactccaat aaaccaaaac
    15841 gccggtgaac gggccttacc tctccatcat ctgcagcttg cgcatcttgc gacgcgccgc
    15901 attgagctta cgtttgcgtc tttcgctcgg tttctcaaag tgctgatgtt ttttaatgtc
    15961 ggacatcaga cctgcttttt cgcaagcctt ggtaaaacgc ttcaaagctc tttcaaaggg
    16021 ttcgttttca cgaatgcgaa ctcctggcat tcagaaatca acctccttcc tgatcgtatt
    16081 tttttgtacc atttacaaat ttaaggaaaa acagccacag aagcaaggag tttttcctgt
    16141 ttgcctcggc cctcccgcta aagccaggag agccccgcgc ttttttattg ctttgttcgc
    16201 gattttgatg tatcctatat taggcggctc cagaaccgca gattggtagg tcaccccttc
    16261 tccggaggaa ccatgttaaa aaaaatcctg ctcgccatcc tgatcctgtt gatcgccggt
    16321 ctggcctatc tctacctcaa taaggacaag atcgcccgtg tggccatcga gaagagcctt
    16381 cccctcatcg aaacctccct gctcgagaac ctgcccggcg acgtcaaccg ggatgatgtc
    16441 aaggcggtgt ttgaccggat tgatgtcaag gtcaaggagg gcaaggtgga catcatgcag
    16501 atgcaaaccc tgctcgaaaa tttccagcaa gccctcaagg accagaaggt cgacgaggag
    16561 gaattccaca aggtctacgc ggagataaaa aagctcgccg gcgactaacg gcagtcatgc
    16621 ccagaggcgc ggcggccgac atttttcttg attttagcgg ccggattggt taccttgccc
    16681 ccaacgcaga tctttcaccc caagggaaag cgcccatgaa gctcaacctt cgccatctga
    16741 tcaggcaaat ctcgccgccg ccgaagtggc ggatccccgt gctcttcctg ctcgccctcc
    16801 tcaccgggct gggcggcctg accctccatc tggccagggc gacctcctat ttctcggacg
    16861 atccgctcac ctgcctcaac tgccatgtca tgcgctcgca gttcgcgacc tggcagcgca
    16921 gtagccacac ccgcgtggcg acctgcaacg actgccatgt gccccacgac tccttcatcc
    16981 gtcactatgc cttcaaggcg caggatggac tgcgccacgc tttcatgttc actttccgtc
    17041 tcgagcccca ggtcatccgc atcaagtcag ccggggccgg cgtggtccgc gagaactgca
    17101 tccgctgcca tcgccagctc gttgaacagc tgccgatgta caagttcgcc gctgccagcc
    17161 cacgccaccg tgtgcccgac aagagctgtg tggaatgcca tgccggcacc ccgcatggcc
    17221 gggtcaacag cctggcctcg gtcccgatgg tgcgtgtgcc tcgccttact ccggccgctc
    17281 ccgcatggct gatgcagccg cgcaatcgcg ccgccgtcga tcctgaacct taatctgcct
    17341 ggagaacatc atgtctggcg accaaaccaa attcacgcaa aaacccctct tttactggat
    17401 gctcttcatc gccaccgcgg tcgtggtttt tctgattggt ctcttcggcg cctctgttct
    17461 cgagcgccgc agcgaatcct atcagctgct gcagccggtc aagcccatcg ccgactggga
    17521 gcccagcaat gccgcctggg gcgaaaatta tccccgtgaa tatgagacct accgcgccac
    17581 ggcggagacc accttcgcct ccctgcacgg aggttcggcc accatcgatg ccctcgagcg
    17641 ctatccccgc ctcgtcgtcc tctgggccgg ctacgccttc agccgcgact acaagcaggg
    17701 gcgcggccac tatcacgccg tcgaggatat ccgcaacacc ctgcgcacaa acgtcccgca
    17761 gccgggcacc tgctggtcct gcaagagcac cgacgtcccg cgcctgatca acaaaatggg
    17821 cgccgccgaa ttctacaagg ccaaatggga ggacctcggc tccgaggtcg tcaacaacat
    17881 cggctgccag gactgccacg atcccaagac catgaacctg cgcatcaccc ggcccgccct
    17941 tatcgaggct taccagcgcc agggcaaaaa gatcgaagag tcgacccacc agcagatgcg
    18001 ctcgcaggtc tgcgcccagt gccacgtcga gtactacttc aagggcaagc aggaaaaata
    18061 tcttaccttc ccctgggacg agggttccac cgccgagcag atacaggcct attatgacaa
    18121 ggtcgagcac gtcgactggg tccacaagat cagcggcgcc cccatgctca aggcgcagca
    18181 ccccgactgg gagatcttcc agatgggcat ccatttccag cgcggcctcg cctgcgctga
    18241 ctgccacatg ccctaccgca gcgagggcgg agtcaagttc accaaccaca agatcgccag
    18301 cccccttgaa aatgtggccg gttcctgcca ggtctgccat cgcgagagcg agaaagccct
    18361 catcgataat gtgcaggagc gccaggagaa ggtgcgccag ctggcgggca tggccgagga
    18421 tcttttggtc agggcccaca tcgaaacccg cgtggccctc gatcgcggcg ccacccccga
    18481 acagatcaag ccggtctatc agctcatccg tcaggcccag tggcgctggg actttgtcgt
    18541 ggccagccac gggggcagct ttcacgcccc gatcgaatgc gcgcgcatca tgggcaacgc
    18601 catcgaaaag gcggccgagg ctcgcaccca gctggccgga ctgctgggcc ggctgggcgt
    18661 ggagctgccg gtggcgatgc cggacatctc caccaaggcc aaggcgcagc gctacatcgg
    18721 actcgacatg gccaggctcg aggcggagaa ggccgatttc aaggccaggg ttatctccac
    18781 ctggggcgag ccggccctct aaccccgacc gatccggcga tggcagaaca aatccttgaa
    18841 tttatcgtcg cgccggccca gggcaaggaa cggctcgacc tctacctcgc ctcccgtctg
    18901 cctgcggtca cccgggccaa gctgaagcgc ctgatcgatg atggcgccgt ctccctggac
    18961 ggccggccca ccaaggccgg ccatctggtg cgcccgggag aaaccatcgt ggtcaccttc
    19021 cccgccgccg cgcccaaagg cgtcgagccc gaggagatcc ccctggatat cctctacgag
    19081 gacgaacacc tcctcgtcgt caacaaggcg gcgggcatgg tggtgcaccc tgcctatgcc
    19141 aatctgagcg gaaccctggt caacgccctg ctcgcccact gccgcagcct ctccagcatc
    19201 ggcggggatg cccgtccggg gctggtccac cggctcgaca aggagacctc cggcctgctc
    19261 gtcgtcgcta aaaacgacct cacccacgtc ggactggccc ggcagctctc cgagcacaaa
    19321 atggagcgcg aataccgggc catcgtctgg ggccacctcg cccgcaagca gggctgcgtc
    19381 cgggccgccc tgatgcgcaa tcccaaggac cggctgcgca tgatgatcca cccggagggc
    19441 aagatggcgg tcacccatta tcaggtgctg gaggagctgc cactcacgag ctatatgcag
    19501 ctcaacctcg aaaccggccg gactcaccag atccgcgtcc atatgagcag cctcggtcat
    19561 ccggtctttg ccgatgccac ctacggggga cgcgccaaac agctggccgg cctgaatcat
    19621 gaaaataccc ggctcggtct gcacctgctg aaaagctgca accggcagat gcttcatgcg
    19681 cgcaccctcg ccttcaccca ccctgtcacc ggcgaattgc agcgcttcga gtgcgccatc
    19741 cccgctgata tgcaggccct tctcgaggag ctgcgcagcc agtcggaggt ataatgcaaa
    19801 ctttttcgcc gcaccggcgt ataatgaaca aaccgatgcc catagcccgg aggcaagcat
    19861 gaaaattgtc cctctcctcg ccctgctcct gtgcgccctg acagccggcg ctgaggccga
    19921 aatcgtctcg atggaaaaaa agtaccatat ccgcggcgat atggaactgg acatgcgcgt
    19981 cgacggcggc aaggtcgaga tcctgcgcag cgaggacaac gactgccata tcctgatcga
    20041 atatgaccgg gagagatgcc gggctgatgt caatcttgat gaagataccg atgtgctcga
    20101 actctttgtc gatcacgaca actgggcgat gaccaaaaaa agcgtcgatg acaaatccaa
    20161 atatgcccgc atccgcatca gcctgccccc caaggctgat ctggcgctga atctggagat
    20221 gaaggccggc gagctcgaca tgaaagtggg cgacctgcac attaaaagct tcgaactccg
    20281 cagctatgcc ggcaagacca gcatcgattt cgaccagccc aaccgctgcg agatgaaaac
    20341 cttcgacgtt gatttcaaga tcggcgaggt caagctctac aatctcggca atgccaattt
    20401 cgccgaagcc gacatcaaca gcagcgtcgg ctccatgctc atcgatttca acggcgaaaa
    20461 aatcgaacag gccatggccc gcatcgatct cgagatcggc gagagcacca ttgtcgtccc
    20521 cgaaaaaatc ggcgtcaaga tcaaggtctc caagttcctc ttcctctcca atgtcgatta
    20581 tcccaactgg ttcacccagg agggctctta ttactattca cgcaactata acaagaaaaa
    20641 ggagtcgctc tatctggtcg tttcgacagg catcggcgaa ctcaagatcc gcgtcgacaa
    20701 gaaaaatgac taaccgctaa tctacgctac tctgattccg ctgccaggag caccacatgg
    20761 cagcccggaa cacccactct tcccgcccgc ccacccactc aaaaccggct aatgggtgaa
    20821 cgcttgatgg aacctgggca accctgatct ggccggactg gagcaaacgg taacagcatg
    20881 atcctcatcc tggttctcct cctcttagcc ttccccctct atggtcaagg cgacctctat
    20941 ctggttttcg gctccgacac cagcatctgg gatggcatga gcacgaccaa ctttgtcagc
    21001 cattaccggc cggacaccta ttccgccccg gccagcaatg cggctgtggt cattgacgaa
    21061 ggctttcgag cccgtttgcg cgactccgag ggccgcccca tgcccttcac ctggtggatg
    21121 cacagcggca atctcttccg ccacgccgac aactgtgatg tcccccttgc caacaccatc
    21181 gccatccatt tgatgaagcg ctatcacggc gatcgcattg ccagggtggg cgacgaactg
    21241 accttgcatt accacacctg ggactggacc gacgagaacg gcgacggcat ctactgctgg
    21301 aaccaaacgc gcagctttga gcagtgccga gaggattttg atctcaccct ctgccactat
    21361 ctgctcgagg aggagatctt tccggtctcc ttcagaagtg gatggcacta tatggacaac
    21421 atctggcagg cctatctcga cgagctgctc cccttttctc tccacagcgc ctatccctca
    21481 aaaaaactga cctcggaaga gccgaccaac aatatctatg actggtcgca agccacctcg
    21541 agatgggtac cctacaaacc ctctcaggat aactatcagc tcccgggcgg ctcgaggggc
    21601 tggaacgtac gctgcatata tatgggcggg ctcaaagccg cggctgtcga gggcatgttc
    21661 gccggggcca acgccggtgt ggaccagctc gcctgcatct ggtcacacct tcccgaagcc
    21721 gatttccctg agcagatcac caaagcccat acgctcatcg tcgaggccgc caaacgctat
    21781 cccaaggtcc gttttcatta catgaccgct atcgacggct accaggcctg gctcaggacc
    21841 gccgaccggc aggccccgac cctcaccttt cgagataacg gcccggcaga gcagccggtc
    21901 ttttcgatag agaccgacga acccatcttc cagagacagc ccttcgtcgc catcaaggat
    21961 atctacagcc gctatcagat tctcccctgc acccgcaccg gcgcatttac ctgggaagcg
    22021 gtatcccccc tcggccgcga ccgcctcgtc aaggccgggg tggcgctttg cgatacggtc
    22081 ggcaaccagg ccctccagct catccgttat ctgccggagg atatttttct ggacaatgaa
    22141 tcgggcgcct atcgcgaaca gactcccctc ttcagaacca tcgacgacta cgcctggggc
    22201 accaacgccc gcgccgccac catcgccggc ggggattcgg ccgtggtccg cttcgagctg
    22261 cccgtgcgcg aaaaacgggc ctaccagctc ctctgtcagt tcgccccgac cgcccatcca
    22321 gctgacaccc tgaccgccat cctcacccag tccggccgca tcatctggcg ggaggaaatc
    22381 accggaccca aagccgccaa acgctggcac tttgtcaccg tctctgaact cgatcccgcc
    22441 ggcgcgccgg ccctgtacat gatcgcacac aaccgcagtg cagagctgcg cgaattcagc
    22501 ctcgacgccc tgagaatcac cccgatcata cccgatcgca agctggaggt cagcccggct
    22561 cttttccatt ttggcgagac cagcctctat acccgcgtcg aaaagaacct gctcatccac
    22621 aacgccggca gcggccccct ctccatccac tccatctcca cccggatggg cctctttcag
    22681 ctcagcacgg cggggcccct gaccatcccc cctttcagca gcgacactct cgcggtggcc
    22741 tttgagtccg accatgctgt aatggcgacc gataccctgg tcatcgccag tgatgatccc
    22801 gtccagccac gccacgtgct gccactcacg gtcgaggcca gcgccttctt cattactgcc
    22861 gataatgacg atcctgtcag ctatttcgag tacggcgtct ggttcaactc ctccgtcgag
    22921 gcctccggcg cgagcagccg ctacatttcc ctcagcggcc atctcggggc atgggcgcag
    22981 ttcatggcca ccccgccgcg cagcggcacc tatgccattt cgatcattct tcccaaaacg
    23041 gaaaatgcca ccaaccatgc cctttacaaa atcctcgccc gcggccagac cctcgattcg
    23101 ctctatatcg atcagaacgc cggcagcggt ggatgggtcg aactcggccg ctacgacctc
    23161 atggcaggaa gacctgttct cgttcaggtc atccatgccg gcggccagac cgccggcgct
    23221 gtgctcaggg ccgacgccgt gcgcttcatc ctgcagggcg aaagcaccgg cctggcccgc
    23281 acggagagcg gcgtgcttcc cggtgaggtc cggctgctgc ccaactatcc caatcccttc
    23341 aatgcttcca caaccatttg ctattcactc ccggctgcgg ccggggtgca tctagaaatc
    23401 tacgatctgc aaggccgcag ggtgcgcacc ctcagccggg aaatccggat ggcgggggaa
    23461 catacggtga cctgggacgg atgcgatgag gcgggaaggg tggtcgcttc gggtctctat
    23521 ttctacaaat tatccgggga ggatttttcg ctctcccgac gcatgtttct ggtccgttaa
    23581 gttcacgtct aggagatgct ttcatgaaac gtattcacca tcttttactg ctcctcatgc
    23641 tcgccgctct gctcctgctg gcgggctgcg ctcctggcac cggccgctac accgccgccg
    23701 ggccggcggg atttctggac ggcatctggc acggctggat cgccccggtc accctcatcg
    23761 tcggcctctt caacgatggc gtgcgcatct atgagccttt caataaaggc tggtggtatg
    23821 atctcggctt ttacatcgct gtgatctcag gcttcggcgg gctttccctg acacgccgca
    23881 aaaagtagtg tgcggagagt tattactggg atccgcaggg agtggctgac ggacatcctt
    23941 gcggcagcac aagtctgcgg acggacaata aaaaaaggcc ggatggtata ccatccggcc
    24001 ttttttttat tgctcatttg ctgcggctcg ggccgtccct ccggcttaag gccgccagga
    24061 cggggagaac aacggactgc cttcagccgc ctgggtctct atcaaccctt gggggtggga
    24121 tgcgcaatct gcttccagtc ctgctccgcc ttgaagggct tgtaggttgg gctctcctga
    24181 ttgtggcact tgacgcagga cttgagatca ccgggattgt aggagactgc agcatggtcc
    24241 tgggtgcccg cagccagggc cttcatgacc ttcatggact ggtagagcga accggggcca
    24301 tggcaggttt cacaggtcac gccgtcttcc attttgtagg tcgccgcctt ggcggtcgca
    24361 ggggcgtcgt aggccgtgac gtggcacttc aggcacttgg atgatttctg gggatcatcg
    24421 atgccggcct ttttgccgac agctttggcc tcttcggtgc ccagggtggc gtaggcgctg
    24481 gcatgcttgg acttggacca gatcggatgg gcctcgccct tggccttggc cgtgtggcac
    24541 atcttgcact tggcggcgcc aatatactgg gctttctcgg tctgagcgaa gagattcata
    24601 ctgaaaacca gggccagaac gaagacaagt ccgaccatcc acaggctttt tttcattttt
    24661 ttcctccggt gtgataataa cggctgtact gacaacacct aaagttaacc aaaaagggat
    24721 gagaattgca ataataaaat ttatcagcat aactgatcgg tacattttgc ctcagtataa
    24781 cgccgataac cggcaaaagt tcattcttcc ggttcattat tttcatcttc tcccccctct
    24841 ccccagcgct ccatgcgcga atagagttgg cggcgggtga tcccgagcag tctggcggca
    24901 aggcttttat tgccctccgc cttttccagg gccttgtcga tgaggcggcg ttccatttcc
    24961 tccagggtcg ggatctcacc gactcctcgc gccggtgccg gagcgttctc cccgctgtgg
    25021 cgcaggtgaa cgggcagatc ctcggcggtg atctgcttcc cccccgacat gatcagggcg
    25081 cgctcgatga tgttctccag ttcgcgcaca ttgccgggcc agttgtactc catgagagag
    25141 tgcagggctc cggcatcgat ccccgccgtt tggctgtttt gtttggccag gaaatgatcc
    25201 gccagcgccg ggatgtcctc cacccggtcg cgcaggggcg gcagcatgat cggaaagaca
    25261 ttgacccggt aatagagatc ctcccggaag gtattgctgc gcacagcctc ttccagatca
    25321 cgattggtgg cg
//
LOCUS       DAOPWU010000012        24560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_2017_length_24560_cov_4.480351, whole genome shotgun sequence.
ACCESSION   DAOPWU010000012 DAOPWU010000000
VERSION     DAOPWU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24560
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2017_length_24560_cov_4.480351"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="PKM23_01615"
     CDS             <1..1490
                     /locus_tag="PKM23_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNY90198.1"
                     /translation="VSVRFEMVPPKPPDDFTSGWRGFNLLGFFTVEWSNDGFYEEDFQ
                     MISELGFNFVRLPIDYRIYTRPGDWSVFLEPKLAQIDQAVAWGQKYGIHVCINLHRAP
                     GYCVNSPSTPLPPRQNISLWDNAEARQAFAAHWRMFAERYKTVPAEALSFNLVNEPGN
                     VSGETYAQAMQPAIQAVRQVSPQRVIISDAVDFGNGRNDAILAQNVVVSPHFYNPFTL
                     THYNADWVDGSDSWPVPVWPPHLVSGYFYGSGKSPWNTPLLISGDFPAGTTVTLHVSQ
                     VSASADFQVYAGSVLQYRHDFRPAAGTGEWKEVIYRPEWNIYQNIYDQDYTFTLTRGA
                     SELCFRVQSGDWMTWTGLTFEMPAGSALKNVTISPGISDWGVPQASYALQPDGSLLLV
                     KVPAGFEEHFLVNGFLQQWIDLKKSGVAVHVGEWGVYNKTPHEVTLAFMENRLLAMKT
                     AGLGWALWNFRGSFGIMDSDRRDVVYENWRGHKLDRKMLELLQRY"
     gene            complement(1592..2074)
                     /locus_tag="PKM23_01620"
     CDS             complement(1592..2074)
                     /locus_tag="PKM23_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90199.1"
                     /translation="MEKTIYLGGYSKGQARGMIILYILLAISYGLQAISIFRDQRPNH
                     LLFSGLFLLCCVGTIVVIFMVRHEKKPQIILQENRLTMRLTPRSGWRKLTSGEIERLH
                     FRPGRVELYLLQKGSDPLNIAASSYDQTRQLKEALREFAEANHIALTEAPFNPPLRRG
                     "
     gene            complement(2275..3729)
                     /locus_tag="PKM23_01625"
     CDS             complement(2275..3729)
                     /locus_tag="PKM23_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNY90200.1"
                     /translation="MDFKLELLIDLAIILIFLAGFRLFRTPKGARSGNLMAAFALILA
                     VVVVILRHRILQPEIVLIALAAGSLVGWIVAMRVNMIQIPAMVAFQHGAGGVAAFLVS
                     FVELTRLAEPGMAKVSGILGLIIGAVTFSGSMIASGKLANVIKQPPVVLPKHSWLTIG
                     LIILLAIGGVIAGGVQGASLVYWLLLLILLSVLLGLVFSIRIGGADMPVLISFLNATA
                     GLAAALCGVIIQNRLLIACGATVAASGSILTHVMCKAMNRNLFSVFFGFQHKSASEKI
                     QAAPTEGPAGEGQSPAPAAAEQPPAPQRVKGDPLTEAVEAAREAKSVIFIPGYGMALA
                     QAQFLVVELAALLEKMGKEVKFAIHPVAGRMPGHMNVLLAEAELPYDKLCEMEDINPE
                     FNRTDLAIVVGASDVVNPAAITVEDTPISGMPILLAHEAGKVLVCNLDERPGYSGIAN
                     PLYQMDRVIALFGDAKASLTSLIEQISGEPVRSR"
     gene            complement(3738..4016)
                     /locus_tag="PKM23_01630"
     CDS             complement(3738..4016)
                     /locus_tag="PKM23_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNY90201.1"
                     /translation="MNALLIMLSVFIVSIIIGYLLISRVPPLLHTPLMSMTNAISAVT
                     ILGALLIFAVTTTITDRVLGVLAIALAAFNVVGGFVITERMLKLFKNK"
     gene            complement(4034..5197)
                     /locus_tag="PKM23_01635"
     CDS             complement(4034..5197)
                     /locus_tag="PKM23_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNY90202.1"
                     /translation="MLIAIPKEIMPEEKRVAAIPETVAKLVKLGYKVAVESTAGDGIF
                     FSDEEYKNAGAEIVTDVQTLYAKADIVLKVKEPKFNEKLGKHEIEIMKDKCILVTFLH
                     PAAPSNHKNIKMIRDKGITSLTMDGIPRTSKAQKMDALTSMSTVTGYKSVLLAANHLA
                     KFVPMITTGIGTVKPAKVLIVGVGVVGLQAVATAKRLGGIVSAVDIRAKAKEEAMSLG
                     AKIEGFDVPEEVSTGKWGYAKALPTDWLNKERELLKPLIQDVDIIILSALVPGEVAPI
                     LLTDEMVASMKPGSVIVDVAIDQGGNCAVTDAGKVVCKHGVTINGLQNIPGRMAVHST
                     WLYANNMYNYVENLFKNGLGVIDFEDEIVKNSLVTHEGKIVHEGTLKALGEVH"
     gene            5717..5917
                     /locus_tag="PKM23_01640"
     CDS             5717..5917
                     /locus_tag="PKM23_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNY90203.1"
                     /translation="METGTVKWFNSSKGYGFITREAGDDVFVHYKNILGEGYKTLDEG
                     DQVRFDVGQGPKGLQATNVTKV"
     gene            complement(6014..8782)
                     /locus_tag="PKM23_01645"
     CDS             complement(6014..8782)
                     /locus_tag="PKM23_01645"
                     /inference="COORDINATES: protein motif:HMM:NF023559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNY90204.1"
                     /translation="MFRIGQRWISEMEPELGLGTVTAADRRVVTIDFPASGCARSFAI
                     ESAPLRRVLFRPGDTITLRDGSAFLVLIAEEQNGLMRYSGETVGAGEEMLADTISFNT
                     PRDRLLNLYIDSNSEYLLRYRTLRHRYHYRRSPLRGLLGGRIELIPHQLYVADTVSSR
                     PDPRVLLSDETGLGKTIEAALILHRLLTVGRINRVLVCVPEPLVHQWFVELLRRFNLI
                     FRLYDAEHIESLLALQSGENPFLEEQLVLCSVELLSSCPEWGELAAGAGWDMLVMDEA
                     HHLVDPGAAYDLARALCAASAGVLLITATPEQLGRTSHFSRLRLLDPAKYRDREEFEK
                     EEKEYTRRVAILNKVIAGERLTAREEERLAALAPAWAAREGAGLARRLREDDARREQF
                     VAEVVDRHGTGRAVFRNTRTEISGFPARHVEIIPLAGERSPAGQIPPDFTGDPRLIWL
                     AHYLRQHRRIKVLLICHSPEEAIAIAPALQKLINIKMTLFHEHLSLLQRDRNASWFAD
                     ADGARLLICSEIGSEGRNFQFCHHLFLFDLPPDPELLEQRIGRLDRIGQSETIEILVP
                     CFTGSGQEVLVRWYHEGLNAFAANLPGAHQIHAALGAELRERIAKPHLAGLDDFISRT
                     RTLREETAAMLQKGQDRLLALNSCRPDKARALQEAIAEMDNANELEKLMLKIFDLYGI
                     IPEEIGKRTWQLNLSLLSQPGFPLPALKREQLVVSFDRRTALNHEEIEFLTWDHPMVT
                     GAIDLMLGREKGNCAVCTLKSLEAKAVLLETVFILECVKHQRTQPDRFLPPTPIRIVT
                     NELLEECSAVWPMQKLSAAARNDPYARLAREESFRLELLPSLLAAAQQQAEQQGLELI
                     HTASERAAAAMDQEIERLHVLSRETGPAEEAIAALRSEKELLLDSLGSARLRLDSLRL
                     IGSVPAAS"
     gene            9028..9705
                     /locus_tag="PKM23_01650"
     CDS             9028..9705
                     /locus_tag="PKM23_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNY90205.1"
                     /translation="MAKSIQTLKTEIVEAGRRVWLRGYVAANDGNISARIDRKSILIT
                     PTGVSKGFLKPADLVVVDMEGRLLSGRKKPSSEVHMHLAIYRERPDVQGICHAHPPTA
                     TGFAVAGIPLDACVLPEVIITLGSIPVIPYGTPGTPGIYEPLLARIREHDAFLLANHG
                     AVTTAGDVIGAYHKMETLEHFAHISLVARQLGRVNVLSETEAEALYALRERFGVTVTA
                     RGKARKK"
     gene            complement(9835..11232)
                     /locus_tag="PKM23_01655"
     CDS             complement(9835..11232)
                     /locus_tag="PKM23_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY90206.1"
                     /translation="MTAKGHPLLKDSKAARWTALLIVSFTMFAGYYMADLMAPLQGML
                     QKQLGWSATDYGWYTGAYGWFNVFFFFLILGGIILDKMGVRFTGLAAAVVMVCGALTK
                     YWAITTHSLDGQSWHILFWTFPAQVWMAALGYAIFGVGVEVAGITVSKVIVKWFKGHE
                     LALAMGLQLAIARMGTALALSTSLPISKAFGTVSAPILISATMLCIGLLAYFVYTFND
                     KKLDASLAAHRKHEEKSADEEFHLSDIGAIVSSRGWWYLAILCALFYSAVFPFLKYAT
                     GLMINKYGVSENLAGLIPGLLPFGTILLTPFFGNLYDRKGKGATIMILGAVLIIIVHS
                     LFALPALSHWLVAVLLMIVLGIAFSLVPSAMWPSLAKIVPEKRLGSAYALIFYIQNMF
                     ALMSVPAFIGWLLDKDCVVSTSGVAEQTVTRYTYTLPMIVFALIGVLSLVFALLLKAE
                     DRRKGYGLQLPNIKS"
     gene            11328..13691
                     /locus_tag="PKM23_01660"
     CDS             11328..13691
                     /locus_tag="PKM23_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNY90207.1"
                     /translation="MKPNTLLVLAVLACAGHLHAQYPGQFRSALKAGIKVPVRARAFD
                     LGAVRITDGPFKHALEMDAAYLLTIEPDRLLHRFHAHAGLPVKGEIYGGWESEGLSGH
                     TLGHYLSACAMHYAGSGDPEFKRRVDYIVGELARCQEARKSGYVGAIPDEDSLFARIA
                     RGEIRSTGFNLNGGWSPWYTVHKVMAGLVDAHLYTGNPVALKVVRGMADWAAAVVNPL
                     SEEQRLAMLNCEYGGMNEVLANLFAITGEEKYLALSRRFHDEFVMGQLAQRIDPMPGK
                     HSNTNVPKAVGAARRYELSGSPADLTIARFFWETMTRHHSYVIGGNSNYEYCGEPDRL
                     NDRLSDNTCETCNTHNMLKLTRHLFCLEPDQELADYYERALYNHILSSQNPADGMMCY
                     FVPLRMGTRKQFSDSFNTFTCCVGTGMENHVKYNEGIWYEAADGGLYLALFIPSVLEW
                     RERGVIITQETAFPAEESVRLTIAVKKPVRFPLYLRRPGWAGEIAVRVNGTATQASLN
                     GKGFLVVERRWRNNDRVEIVMPMRLRTEAMPDNPDRVALLYGPVVLAGQLGDTLPDPV
                     YGTPVLLTGKRNPAEWIQPAGAPFTFRTAGIGMPFDFDLRPFYSTADDYYSVYFDYFT
                     PEAWEARKEAYQAEIRRQQEIASRTIDHFRIGEMQPERDHRLEAGERSYVDQALGRSG
                     REARAGSWFSFEMAVRPELPNALLLTLIGDDRDRKFDLLVEGQKIAEIDWRGGETGRF
                     YDLIYPLPEELLCGKSSVTVRIEANHGRTAGRIFALRTIRNNAPLQP"
     gene            complement(13765..15387)
                     /locus_tag="PKM23_01665"
     CDS             complement(13765..15387)
                     /locus_tag="PKM23_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNY90208.1"
                     /translation="MNEPIPASIFPDPEAVPEAWRLAVPVDQRSWLCGGELRTWTGLT
                     QPVLSPICHPGPSGAERAVIGSYPLMSAAESLQVLEAASRAWDQGRGPWPTMPVEERI
                     HHVAAFSARMQEQRGEIVKRLMWEIGKNQVDAEKEFDRTIEYIRDTISALKELDRLSS
                     RFVIEEGVIGQIRRSPLGVVLCMGPFNYPLNETFTTLIPALIMGNPVIFKPPKLGVLL
                     FEPLLAAFRDCFPPGVINTIYGDGATIITPLMESGRIDVLAFIGTSRVADILRRAHPK
                     PHRLRCVLGLDAKNPAIILKDADLDLAVKECVLGALSFNGQRCTALKMIWVHAEVCDL
                     FLEKLSAAVAALKCGMPWEPGVQITPLPEEGKTGRLYEYIRDAEERGARVINPGGGSV
                     QGTCMTPAILYPVSAGMRLYTEEQFGPLIPVASFSEIGEPLHYISDSNYGQQVSLFGN
                     DAKVLAGLIDPLVNQLCRVNLNSQCQRGPDSFPFTGRKDSAEGTLSVSDALRVFTIRA
                     LVAAKENSANQGIIREIVTERTSRFLSTDFIF"
     gene            complement(15404..15727)
                     /locus_tag="PKM23_01670"
     CDS             complement(15404..15727)
                     /locus_tag="PKM23_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90209.1"
                     /translation="MQKILLGIAFALGGIITYVATRPEIHFGGIAIALFVIAFLFGLI
                     QSKYPWLFALALGIWIPVLVLFQGFFKGLFRVQNIWAILALLIAFAGAYAGAFVRNKF
                     TAKTR"
     gene            complement(15750..17258)
                     /gene="fucK"
                     /locus_tag="PKM23_01675"
     CDS             complement(15750..17258)
                     /gene="fucK"
                     /locus_tag="PKM23_01675"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: protein motif:HMM:TIGR02628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HNY90210.1"
                     /translation="MSSRKPAALVLDCGATNVRAILVDAGGRLLASASRANAVHPDPG
                     WPGGLIWDQEEIWAGFCACTRELLSRAPEARIAAITVTTFGVDGAPVHKDGTLLHPVI
                     SWQCGRTRSIQEKIASSIDPLRLFRITGLQSYPFNTLFRLLWFRENRPGVLEGMDYYA
                     MMPSLFIHRLTGEWLTDVTMAGTSMLTDQSRRTFSEELLALAGLDADRFPALAEPGTI
                     AGPLLPAAAAEFGLAARIPVVIAGHDTQFALFGAGGGPTLPVLSSGTWEILMARSTPI
                     HLEGLDPAAGITIEFDARPGLCNPGMMWIASGILEWLGGLLYSDCGGAERYEKMTGEA
                     SLLPPGSEGVGMLPGLFDSSGLRGVYSGLFPGSTRAHLYRAALEALAFRARESLIRLE
                     EWGGFRAGAVICAGGGSKNHLWNRIRADILGIPVLVSPVSETTVLGAAMFALAGAGFF
                     STPEEAARAMQPELTTIEPGENLPAYVEFQKTYRGLLHHLDKNPAARQESVS"
     gene            complement(17315..19093)
                     /locus_tag="PKM23_01680"
     CDS             complement(17315..19093)
                     /locus_tag="PKM23_01680"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HNY90211.1"
                     /translation="MNARHQSRPVKIGIRPAIDGRRKGVRESLENQTMAMAKSVAALL
                     SQNLRHPSGEPVECLIAESCIGGVAEAARCADYFDREGAGVSITVTPCWCYGSETMDM
                     DPFRPKAVWGFNGTERPGAVYLAAVLAAHTQMGLPAFGIYGRDVQDADNQTIPADVAE
                     KLLRFARAGLAIAAMRGTSYLAMGSVSMGIAGSMVDPSFFRNYLGMRNEYVDMSEFTR
                     RMEEGIFDPEEYRAAYAWTRAKCLEGPDHNKARRTPAEKQRIWEKVVQMTLIARDLMA
                     GNPRLAALGFGEEALGHNALLAGFQGQRQWTDHSPNGDFMEAILNSSFDWNGTRQPYL
                     VATENDALNGVAMLFGHLLTSTAQMFSDVRTYWSPAAIRRVTGHTLSGAAEHGVIHLI
                     NSGASALDFSGRQSVAGQPAVKPFWEITPAEVEACLAATLWPPAIYEYFRGGGYSSEF
                     LTRGGMPLTMSRVNLVQGLGPVLQIAEGWSVDLPDEVNKALVDRTDPTWPTTWFAPRL
                     TGKGAFSDVYAVMGNWGANHGAISCGHIGADLITLAALARIPVCMHNVAEAEIFRPSA
                     WNAFGMDKEGADYRACANFGPLYGKR"
     gene            complement(19229..20077)
                     /gene="folD"
                     /locus_tag="PKM23_01685"
     CDS             complement(19229..20077)
                     /gene="folD"
                     /locus_tag="PKM23_01685"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNY90212.1"
                     /translation="MSGMILDGKALARQMEQELAVRVEKIKNISGKTPILATILVGDD
                     PASATYVRMKGNACQRVGMESLKVILPQSTTTERLLEEIDALNANPDVHGILLQHPVP
                     AQIDERACFDRIALSKDVDGVTALGFGRMALGEPAYGSATPAGIMRLLAHYEIPLKGK
                     EAVVVGRSPILGKPVAMMLLNAHATVTICHSRTQNLAEVVRRADIIVGAVGKPEFIRG
                     EWIKPGAVVIDAGYHPGNIGDIELAAVVESCSAYTPVPGGVGPMTIATLMAQTVEAAE
                     KSAGID"
     gene            complement(20096..21823)
                     /locus_tag="PKM23_01690"
     CDS             complement(20096..21823)
                     /locus_tag="PKM23_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNY90213.1"
                     /translation="MSNPNLKFGLERYTLDCFFAEVLNRYPQRPALALVGDEPFTYAE
                     FGRRVADLQARLGELGVKSGVKVALLGGSSPNWCIAFMAVTTMGAVAVPILEDFPEAD
                     INHILHHSDASVIFISDHLHASLNLHALIEGFLVFSLTDLALVSGAGARPGFWKGLQE
                     FGGKVLGKRAKMDPELPAAIGEEDLAEIIYTSGTTGHSKGVMLSHRNLVSNLFEGPDL
                     IKCIHERSVLLSILPLAHTFGSTSGFLSIIREGSLIYFLTKPPAPKVLLAAMQEVKPT
                     IFGAVPLVFEKIYHKQVVPTIAASRLLRMLSKSRASRKLLYKIIGKKVNKLLGGRLEC
                     VIIGGAAFSPEVETFMKQGRIPYCCGYGLTECSPLVTFSSMEEQKMGSPGHAITGVSI
                     HIADPDPTTGIGEILVKGPNVMKGYYKNEEATRKVFTGDGWFISGDRGYLDKDGFLFI
                     TGRSKNVIVGPSGENIYPETIEARLLESIYVEEALVYMTEGQLVARIFPNYSYIESIE
                     GDRAEGAVARAINEILEEVRREVNTRLPAFARISRVIEQSTPFVKTPTNKIKRGEYVP
                     GYLNDDRNK"
     gene            22068..22958
                     /locus_tag="PKM23_01695"
     CDS             22068..22958
                     /locus_tag="PKM23_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90214.1"
                     /translation="MHNSLMRWLGLVMILIVAPGIAAQDLQDYLSRYLGDNGKKYLQP
                     LGDAFGANLNSGLYHTAHIPRVGLHVGVEVVATGALISDAQRSFTATTEEGFEPKKSL
                     EVPTVFGDTAPVAVPGQGGTVYNFPGGYDLSVMPLAMPQITIGSLLGSEVTLRYAAAE
                     LNEEVGDLSLLGIGFRHSLSQYIPLFPVDVAAAVFYQTLTVGDILDAKTRVIGLSASK
                     TFNLFTLYGGLSHENSVMEASYEHGEGEEAETISFDLEGANSVRLTLGATLKLAIFQL
                     HADYSFAAQQSACLGLYIGL"
     gene            22996..23589
                     /locus_tag="PKM23_01700"
     CDS             22996..23589
                     /locus_tag="PKM23_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90215.1"
                     /translation="MNKRIGLVLLAGAALLMSGCQKKLFHFVVPLNKVKTIAINNTGE
                     FAGYEKITARDIRAALDVPEDGTITGVDLESVQIRVRLVEGNVATWVKLSGFVLDQGK
                     PVQLFEDKSVPLSGVDTKYIGLNALIARGVEKLAGKINDHVKKVNDADIEIGLTGNSD
                     PAGQLIKVEIDFKINATVKYDQCVEVFDFIGGEDCTE"
     gene            23798..>24560
                     /locus_tag="PKM23_01705"
     CDS             23798..>24560
                     /locus_tag="PKM23_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY90216.1"
                     /translation="MTHRCTPLLACILACCLLTGCRRDPGEQLLLDFWAMGAEGENIQ
                     PLLPGFARRHPEITVRVQTVPWGAAHDLLLTAFAGGSSPDLCQLGNTWIPEFAAIGAI
                     RPLDPFLADSTLRPGDYFPGIWETNCIGGRVYGIPWYVDTRLLFYRSDLLAAAGYPRP
                     PRDWGEWLDLSRRLVQQQPGRYALFLSTIFNDWQVPAILILQNGGRLLRENDCYGAFS
                     EPATLAALRYYITFFHDSLAVINMSEVANIFQGFSE"
BASE COUNT         4763 a         7295 c         7783 g         4719 t
ORIGIN      
        1 cggtctcggt gcgcttcgag atggtgccgc ccaaacctcc cgacgacttt acatcgggct
       61 ggcgcggctt taatctgctc ggatttttca ccgtggaatg gagcaacgac ggcttttacg
      121 aagaagattt tcagatgatc tccgagttgg gctttaattt tgtccgcctg cccatcgatt
      181 accgcatcta cacccggccc ggtgactggt cagtctttct tgaacccaaa ctggcccaga
      241 tcgaccaggc ggtcgcctgg ggacaaaagt atggcattca tgtctgtatc aacctgcacc
      301 gggccccggg ctactgtgtc aattcgcctt cgactcccct tccgcccaga cagaatatca
      361 gcctctggga caatgcagaa gcccggcagg cctttgcggc ccactggcga atgtttgccg
      421 aacgctataa aacggttcct gccgaggccc tcagcttcaa cctcgtcaac gagccgggta
      481 atgtttccgg tgagacctac gcccaggcca tgcagcccgc cattcaggct gttcggcaag
      541 tctccccgca gcgcgtcatc atttccgacg ccgtcgattt cggcaacggc cgcaacgacg
      601 ccatcctcgc acagaatgtt gtggtttcac cccatttcta caatcccttc actctcaccc
      661 attataacgc agattgggtg gatggctcgg actcctggcc ggtgccggtc tggcccccgc
      721 atctggtttc cggttatttt tacggatctg gcaagagtcc ctggaataca cccctgctca
      781 tttcaggaga ctttccggcg ggcacaaccg tcaccctgca cgtctcccag gtttcggcca
      841 gcgcagattt tcaggtctac gccgggagcg tattgcagta tcggcacgat tttcgcccgg
      901 cggccggaac aggagagtgg aaagaggtga tctatcgccc ggaatggaat atctatcaga
      961 atatttacga ccaggactat accttcacgc tgaccagagg ggcgagcgag ttatgcttcc
     1021 gcgtccagtc cggcgactgg atgacctgga ccgggctgac ttttgagatg ccggcgggat
     1081 cggcgctgaa gaatgtgacc atcagccccg gcatctccga ttggggcgtg ccccaggcct
     1141 cctatgccct gcagccggac ggttcactcc tgctggtcaa ggtcccggcg ggattcgagg
     1201 agcattttct ggtcaatggt tttttacaac agtggatcga tctgaaaaag tcgggggtag
     1261 cggttcatgt cggcgaatgg ggcgtctaca acaaaacgcc ccacgaggtc acgctggcct
     1321 tcatggaaaa ccgcctgctg gcgatgaaga cggcgggact cggctgggcg ctctggaatt
     1381 tccgcggcag ctttggcatc atggacagcg accgcaggga tgtcgtctac gaaaactggc
     1441 gcggtcacaa actggaccgg aagatgctcg agcttttgca gaggtactga ttcgcatcat
     1501 tgggaacctg cggagcggat atacgaggga cagctcatcg agtatcggat cagattcatc
     1561 aagggcttgt ggttaaaatg gctgacctgg atcacccacg tctgagcggc ggattgaaag
     1621 gggcttcggt gagcgcgatg tggttggctt cggcaaactc gcgcagagcc tccttcagct
     1681 gtctggtctg atcataggaa gaggctgcga tattgagggg gtcgctgccc ttctgcagca
     1741 gatagagttc cacccgcccc ggtcggaagt gaaggcgttc gatctctccg gaggtgagtt
     1801 tacgccagcc cgatctgggg gtgagccgca tggtcaacct gttctcctgc aggatgatct
     1861 gcggcttttt ttcgtgccgg accataaaga tcacgacgat cgtgcccaca cagcagagaa
     1921 ggaagaggcc ggagaaaaga agatgattgg ggcgctggtc gcggaatatt gaaatggcct
     1981 gaaggccata ggagatggcc agcaggatat agaggatgat catgccccgt gcctggccct
     2041 tgctgtaacc gccaagatag atagtttttt ccatgtctga ccccctgcat tggtctcgac
     2101 actttggccc tccctgcgcg cagcggctgg agagggctcc cctcaaataa cttaacctat
     2161 tttgaggcat gagtcaagcg aaaatgttcg ttttgtgaaa cgggatcagc cgccaaaatg
     2221 aaaacagggg gagggccatc ggccctcccc ctgtttcgca tcaacagcag ggtttcatct
     2281 ggagcgcacg ggctcaccgg agatctgctc gatcaggctg gtcagcgaag ccttggcatc
     2341 gccgaagaga gcgatgaccc tgtccatctg atagaggggg ttggcgatgc ccgaatagcc
     2401 cggcctctcg tcgaggttgc agacgaggac cttgccggcc tcgtgggcga gcaggatggg
     2461 catgcccgag atcggggtgt cctccacggt gatcgccgcc ggattgacca catcgctggc
     2521 gccgacgacg atggcgagat cggtgcgatt gaactccgga ttgatatcct ccatctcgca
     2581 gagtttgtcg tagggaagct ccgcctctgc aaggaggacg ttcatgtgtc cgggcattct
     2641 gccggccacc ggatggatgg caaatttgac ctctttgccc atcttttcca gcagggcggc
     2701 gagctcgacg accaggaact gggcctgggc gagggccatg ccgtagcccg ggatgaagat
     2761 aaccgatttg gcctcacggg cggcctcgac ggcctcggtg aggggatcgc ccttgacgcg
     2821 ctgcggagca gggggctgct ccgcggccgc cggagccggg ctctgacctt caccggctgg
     2881 cccctccgtc ggggcagcct ggattttctc gctggcgctc ttgtgctgga agccgaagaa
     2941 gacgctgaag aggttgcggt tcatcgcctt gcacatgacg tgggtgagga ttgaaccgga
     3001 ggcggccaca gtcgccccgc aggcaatgag cagacggttc tggatgatga caccgcagag
     3061 ggcggccgcc agacccgcag ttgcattgag gaacgagatc agcaccggca tgtcagcgcc
     3121 gccgatgcgg atcgagaaga ccagaccgag cagtaccgag agcagaatga ggagcagcag
     3181 ccagtagacc aggcttgcac cctgcacccc gccggcgatg acgccgccga tggcgagcag
     3241 aatgatgagc ccgatggtca accagctgtg tttgggcagg accaccggcg gctgcttgat
     3301 gacattggcg agcttgccgc tggcgatcat gcttccgctg aaggtgactg ctccgatgat
     3361 gagtccgaga atgcccgaca ccttggccat ccccggctct gcaagcctgg tcagctccac
     3421 aaaagagacc agaaaggcgg cgacaccccc cgcaccatgc tggaaggcca ccatggcagg
     3481 gatctggatc atgttgacgc gcatggcgac aatccagccg accagggaac ccgcagccag
     3541 ggcgatcagg acgatctcgg gctgcaggat ccgatgccgc agaatgacca ccaccaccgc
     3601 caggatgagg gcgaaggcgg ccatgagatt gccgctgcgc gcccccttgg gagtcctgaa
     3661 aagccggaat cccgcgagaa atatgagaat gatggcgaga tcgatgagca gttcgagctt
     3721 gaaatccatt gggtcctcta tttgttcttg aagagcttga gcatccgttc cgtgattaca
     3781 aatccgccga cgacattgaa tgcggccagt gcgatcgcca gcacgcccag aacccggtcc
     3841 gtgatggtgg tcgtgaccgc aaagatgagc agggcgccga ggatggtcac cgccgagatg
     3901 gcattggtca tcgacatcag cggcgtgtgc aacaggggcg gtactcgcga aatcaggagg
     3961 tacccgatta tgatggaaac aataaaaacc gacagcatga tcaataatgc attcatagag
     4021 cggcctgcct caattaatga acttcaccga gggccttgag cgtcccttca tggacaatct
     4081 ttccttcgtg ggtgacgagg gagttcttga cgatctcatc ctcgaaatcg atcacgccca
     4141 ggccgttctt gaagagattt tccacatagt tatacatgtt gttggcgtaa agccaggtgg
     4201 aatggaccgc catgcggccg gggatgtttt gcagtccgtt gatggtgacg ccgtgcttgc
     4261 agaccacctt gccggcatcg gtgacggcgc agttgccgcc ctggtcgatg gccacatcca
     4321 cgatcacgga accgggcttc atcgaggcga ccatctcgtc ggtgagcagg atcggggcga
     4381 cctctccggg tacgagggcg ctgaggatga tgatgtcgac atcctggatg agcggcttga
     4441 gcagttcgcg ctccttgttg agccagtcgg tgggcagggc cttggcatag ccccatttgc
     4501 cggtggagac ctcttccggc acgtcaaaac cctcgatctt ggcgccgagg ctcatggcct
     4561 cttccttggc cttggcacgg atgtcgacgg ccgagacgat gccgccgagt ctttttgcgg
     4621 tggccaccgc ctgcagaccg accacgccga cgccgacgat gagaaccttg gccggcttga
     4681 cggtgccgat gccggtggtg atcatcggta caaatttggc cagatggttg gcggcgagga
     4741 ggaccgactt gtaacccgtc accgtgctca tcgaggtcag ggcatccatt ttctgggcct
     4801 tggaggtgcg gggaatcccg tccatggtga gggaggtgat gcctttgtcc ctgatcatct
     4861 tgatgttttt atggttggag ggggcagccg ggtggaggaa ggtgaccaga atgcatttgt
     4921 ccttcattat ctcgatctca tgcttgccca gcttttcgtt gaatttgggt tccttgacct
     4981 tgagaacgat gtcggccttg gcatagagag tctgcacatc agtgacgatc tcggcaccgg
     5041 cgtttttata ttcctcgtcg ctgaaaaaga tcccatcacc ggccgttgat tctaccgcca
     5101 ccttgtagcc cagcttgacc agcttcgcga cggtctcggg aatggcggcc actctttttt
     5161 cttccggcat gatttccttg gggattgcga taagcatggc aaaactcctt tattttttaa
     5221 aatacacccg taacgcggtt aatgagggca gcgatcgctg tcagcgactg cttccggatg
     5281 atggcctccg gctgcaacta ttcagcagaa aagttaggtt attttttagt tatcatcaag
     5341 caaaatattc gccgtttcag gcccggcagc cggccggccg gtgtccaaaa tcaaggcaat
     5401 gctgttccag tcgcttccac ggctgataaa cgctaactct attaatttta ccttgtatgc
     5461 gaattaaaaa aatggtaaaa aaagcaaaaa tcgcttgcta ttataattac cattttgtac
     5521 tttactttag taaccgtttt cacgctttca ggccttttat ttgaaactgg taatccggct
     5581 gtttttcaaa accgtacacc ctcacatatc gtagcgatgg gcgccagacg cttctgctgc
     5641 gattttttat ttgcggagca cggggaggcc gaatcggcgt ggcaggcagc aaacgtctta
     5701 aggaaaggta gattcgatgg aaaccgggac tgtgaaatgg ttcaacagct ccaaaggcta
     5761 cggctttatc acccgcgaag cgggggatga cgttttcgtg cattacaaga acatcctggg
     5821 cgagggctac aaaactctgg atgaaggaga tcaggtgcgc ttcgacgtcg gccagggccc
     5881 caaaggcctc caggccacta atgtcaccaa agtttgactg gacataaccc cgatttagcc
     5941 ccggcgatcc gcagccgggg cttttttttt gcccgcagtc gcagtcttgg cattgatgcc
     6001 gcggaggcgg ggttcaactg gcggcgggaa cgctgccgat gaggcgcagg ctgtcgagac
     6061 gcagccgggc gctgcccagg gagtccagca gcagctcttt ctcgctgcgc agggcggcaa
     6121 tggcctcctc tgcgggaccg gtctcgcggg agaggacgtg cagccgctct atctcctggt
     6181 ccatggccgc ggcggcgcgc tccgaggcgg tgtgaatgag ttcgaggccc tgctgctcgg
     6241 cctgctgctg cgctgcggcc agcagggagg ggagaagctc gagccggaag gattcctccc
     6301 gggccagccg ggcatagggg tcattgcggg cggcagcgga gagcttttgc atgggccaga
     6361 cggctgaaca ctcctccagc agctcgttgg tcacgatccg gatcggggtc ggcggcagaa
     6421 accggtcggg ttgagtgcgc tgatgcttga cgcattcaag gataaagacc gtctccagca
     6481 gcaccgcctt cgcttcgagc gatttgaggg tgcagacggc gcagttgcct ttttcgcgcc
     6541 ccagcatgag gtcgatcgcc ccggtgacca tgggatggtc ccaggtcagg aattcgatct
     6601 cctcatggtt gagggcggtg cgccggtcga agctgaccac cagctgctcc cgtttgaggg
     6661 cggggagggg aaagccgggc tgggagagca gggagagatt gagctgccag gtgcgtttgc
     6721 cgatctcttc ggggatgatg ccgtagaggt cgaagatttt gagcatcagt ttttcgagtt
     6781 cattggcgtt gtccatctcc gcgatggcct cttgcagggc gcgcgcctta tccggccggc
     6841 aggagttgag ggcgagcagg cggtcctggc ctttttgcag catcgcggcg gtctcctcgc
     6901 gcagggtgcg tgtgcggctg ataaaatcgt cgagccccgc caggtgcggt ttggcgatgc
     6961 gttcccgcag ctccgcgccc agggcggcat ggatttgatg ggctccgggc agattggctg
     7021 cgaaggcgtt gaggccttca tgataccagc gtacgagcac ctcctggccg ctgcctgtaa
     7081 agcaggggac gaggatctcg atggtctcgc tctggccgat gcggtcgagg cggccgatgc
     7141 gctgctcgag cagttctgga tcggggggca gatcgaagag aaaaaggtgg tggcagaact
     7201 gaaaattacg gccctcgctg ccgatctcgg agcagatcag caaacgcgcc ccgtcggcgt
     7261 cggcgaacca tgaggcattg cggtcgcgct gcagcagcga gaggtgctcg tggaaaaggg
     7321 tcatcttgat gttgatcagc ttttgcaggg ccggcgcgat ggcgatcgcc tcctccgggg
     7381 agtggcaaat cagcagcacc ttgatgcggc ggtgctggcg cagatagtgg gcgagccaga
     7441 tcaggcgggg atcgccggta aaatcgggcg ggatctgacc ggccgggctg cgctccccgg
     7501 caagggggat gatctcgaca tggcgcgcag gaaaaccgct tatctccgtg cgagtgttgc
     7561 ggaagacagc gcggccggtg ccgtggcggt cgaccacctc ggcgacgaac tgttcccgtc
     7621 tggcgtcatc ctcccgcagc cgccgggcga gtccggcccc ttcgcgcgcc gcccaggccg
     7681 gcgccagcgc ggcgagccgc tcctcctccc gcgcagtcag ccgctcgccg gcgatgacct
     7741 tgttgagaat ggcgacccgc cgggtgtact ccttttcctc cttttcgaac tcctcccgat
     7801 cgcggtattt cgccggatcg agcagccgca ggcgcgagaa atgactggtc cggcccagtt
     7861 gttcgggcgt ggcggtgatc agcagcaccc cggcggaggc ggcgcaaagc gctcgcgcca
     7921 aatcgtaggc cgcccccgga tcgaccagat gatgcgcctc gtccatgacc agcatgtccc
     7981 agcccgcccc ggcggccaat tccccccatt cgggacagct gctcagcagc tccacgctgc
     8041 agagcaccag ctgctcttca agaaagggat tctcgccgga ctgaagggcg agcagggatt
     8101 cgatatgctc cgcatcgtag aggcggaaga tgagattgaa gcggcgcaga agctcgacaa
     8161 accactggtg caccaggggc tcgggtacac agacgaggac gcggttgatg cggccgaccg
     8221 tgagcagccg gtgcaggatc agtgcggcct cgatggtctt gcccaggccg gtctcgtctg
     8281 agagcagcac ccgcggatcg gggcgggagg agacggtgtc tgccacatag agctggtgcg
     8341 ggatcagctc gatgcggccg ccaaggagcc cgcgcagggg ggaacggcga taatggtagc
     8401 ggtgacgaag ggtgcggtag cgcagcagat attcggaatt gctgtcgata tagaggttga
     8461 gcagacgatc tcgcggggta ttgaagctga tggtgtcggc gagcatctct tcgccggcgc
     8521 cgaccgtctc accgctgtag cgcatcaggc cgttttgctc ctcggcgatg aggaccagaa
     8581 aggcgctgcc gtcgcggagg gtgatggtgt ctccgggtcg aaagaggacg cggcgcagtg
     8641 gggccgattc gatggcaaag ctgcgggcgc agccgctggc gggaaaatca atcgtgacca
     8701 cgcggcggtc ggctgcagtg actgttccca gccccagctc gggctccatt tcgctgatcc
     8761 agcgttgacc gatcctgaac atggttgtcc tgattctcca gcgggtagcg actcgtgccg
     8821 gcctgcgcgc ggcggcagcc gccggcggag gatcctcccc cgggtggatc tcttgaaaaa
     8881 tatagcgtta attcatcaga aaaacaagcc ctttcaccgc cacggggagg cccgtttgtg
     8941 gggcggcccg actttaactt gcttctttcc tttcgccttg gtacattgga ctgtagaaac
     9001 agcaggaata gaattggagg caggatcatg gccaaatcca ttcagacatt gaagacagaa
     9061 atcgtcgaag cggggcggcg cgtctggctg cgcggctatg tcgccgccaa cgacggcaac
     9121 atcagcgccc gcatcgatcg caagagcatt ctgatcactc cgaccggggt gagcaaggga
     9181 ttcctcaagc ccgcggatct ggttgtggtc gatatggagg ggcgcctcct ctccggcagg
     9241 aaaaagccct cctccgaggt gcacatgcat ctggccatct accgggagcg gccggatgtg
     9301 caaggcatct gccatgccca tccccccacc gcgaccggct ttgcggtggc cggcatcccg
     9361 ctcgatgcct gcgtcctgcc cgaggtgatc atcaccctcg gcagcatccc ggtcatcccc
     9421 tacggcaccc cgggcacgcc gggcatctac gagccgctgc ttgcgcgcat cagggagcat
     9481 gatgcctttc tcctcgccaa ccacggcgcg gtcaccaccg ccggcgatgt gatcggcgcc
     9541 taccacaaga tggagaccct ggaacatttt gcgcacatca gcctggtcgc tcgccagctc
     9601 gggcgggtca acgtcctgag cgaaaccgag gcggaggccc tgtatgccct gcgcgaacgc
     9661 ttcggcgtca cggtgaccgc caggggcaag gcgcgcaaaa agtagccagc cgaatccggc
     9721 accgataaaa aaaagccccg gcttgaatgc ccggggcttt ttgttttatg gtgatcctcg
     9781 cacagccgcg gctgggatgc tgcaagcggc tctgctgcgg gttctcgtcc gctttcagct
     9841 cttgatgttc ggcagctgca ggccgtagcc ctttcttcgg tcctccgcct tgagcaggag
     9901 ggcgaagacc agtgagagca ccccgatcag ggcgaaaacg atcatgggca gggtataggt
     9961 atagcgggtc accgtctgct ccgccacgcc gctggtcgaa accacgcagt ccttgtcgag
    10021 cagccagccg atgaaagccg gcacgctcat cagtgcgaac atgttctgga tgtaaaaaat
    10081 gagggcatag gcgctgccca gcctcttctc ggggacgatc ttggccaggg agggccacat
    10141 cgccgagggg accagggaga aggcgatgcc gagtacgatc atgagcagga cggcgaccag
    10201 ccagtggctg agcgccggca gggcgaagag ggaatggacg atgatgatca gaaccgcgcc
    10261 gaggatcatg atcgtcgccc ccttgccctt gcggtcgtag agattgccga agaagggggt
    10321 gagcaggatg gtgccgaagg gcagcagtcc cgggatgaga ccggcgagat tttccgagac
    10381 gccgtacttg ttgatcatca gaccggtggc atatttcagg aaaggaaaga ccgccgaata
    10441 aaagagggcg cacaggatgg ccaggtacca ccagccgcgg ctggagacaa tggccccgat
    10501 gtcgctgagg tggaactcct catcggcact tttctcctca tgcttgcggt gggcggccag
    10561 tgaggcatcg agtttcttgt cattgaaagt gtagacaaag taggcgagca gaccgatgca
    10621 gagcatggtt gcggagatga ggatgggggc gctgacggtg ccgaaggcct tggagatggg
    10681 cagggaggtg gagagggcca gggcagtgcc catgcgggcg atggccagct gcagtcccat
    10741 cgccagggcg agctcatgcc ccttgaacca cttgacgatc accttggaga cggtgatgcc
    10801 cgcgacctcg acgcccaccc cgaagatggc gtagccgagg gcggccatcc agacctgcgc
    10861 cgggaaggtc cagaagagaa tgtgccagct ctggccgtcg aggctgtggg tggtgatggc
    10921 ccagtacttg gtgagggctc cgcagaccat gacgacagcg gcggcgaggc cggtgaagcg
    10981 cacccccatc ttgtcgagga tgatgccgcc gaggatgagg aagaagaaga agacgttgaa
    11041 ccagccgtag gccccggtgt accagccgta atcggtggcg ctccagccca gctgtttctg
    11101 cagcatgccc tgcagggggg ccatgaggtc cgccatgtaa tagccggcga acatggtgaa
    11161 ggagacgatc agcagagcgg tccagcgggc cgctttggaa tctttgagaa gcgggtggcc
    11221 tttggcggtc atgggattct cgcaaagttt gggttggatt atttcgcaca taatagctaa
    11281 attggtggag aattgcaagg ccttttcgct tcccgcaagg agagttcatg aaaccgaaca
    11341 cccttctcgt tctggccgta ttggcctgcg ccggccacct gcacgcccaa tatcctggcc
    11401 agttccgctc cgccctcaag gccggaatca aggtccccgt ccgggcccgg gcctttgacc
    11461 tcggtgcagt ccgtatcacg gacggcccct tcaaacatgc cctggagatg gatgccgcct
    11521 acctgctcac catcgaaccc gaccgtctgc tccaccgctt tcatgcccac gccggcttgc
    11581 cggtcaaggg ggagatttac ggcggttggg agagcgaggg gctctccggc cacacccttg
    11641 gccactatct ttccgcctgc gccatgcact acgccggcag cggcgacccc gaattcaaaa
    11701 ggcgggtcga ctatatcgtc ggagaactcg cccgctgcca ggaggcgcgc aaatccggtt
    11761 atgttggtgc gataccggat gaagacagcc tcttcgcccg catcgcccgg ggcgagatca
    11821 ggtcgaccgg ctttaacctc aacgggggct ggtccccctg gtacaccgtc cacaaggtga
    11881 tggctgggct ggtggatgcc catctgtaca ccgggaatcc agtcgctctc aaggtggtgc
    11941 gcggcatggc cgactgggcg gccgctgtgg tcaatcccct cagcgaggag cagcgcctgg
    12001 ccatgctcaa ctgcgaatat ggcggcatga acgaggtgct ggccaatctc ttcgccatca
    12061 ccggagaaga aaaatatctc gcgctctccc gccgcttcca tgacgagttt gttatgggac
    12121 agctggcgca gcgcatcgat cccatgcccg gcaagcactc caacaccaat gtgcccaagg
    12181 cggtaggcgc cgctcgccgt tatgagctgt ccggcagccc cgcggacttg accatcgccc
    12241 gttttttctg ggagaccatg acccggcacc actcctacgt catcgggggc aacagcaact
    12301 atgaatactg cggcgagccg gaccggctca acgatcgcct cagcgataat acctgcgaga
    12361 cctgcaacac ccacaatatg ctcaagctca cccgccacct cttctgcctt gagcccgatc
    12421 aggagctggc cgactattac gaacgcgccc tctacaatca catcctctcc agccagaatc
    12481 cggccgacgg catgatgtgc tacttcgtcc ccctgcgcat gggcacccgc aagcagttca
    12541 gcgattcctt caacaccttc acctgctgcg tcggcaccgg catggagaac catgtcaaat
    12601 acaacgaggg catctggtac gaggcggccg acggcgggct ctatctcgct ctcttcatcc
    12661 cttcggtgct ggagtggcgg gagcggggcg tcatcatcac ccaggagacc gcctttccag
    12721 ctgaggagag tgtgcgcctc actatcgcgg tgaaaaagcc ggttcgattt cccctctacc
    12781 tgcgcaggcc cggctgggcc ggggagatcg cggtcagagt gaacggaacc gccacacagg
    12841 cctccctgaa cggcaaggga tttctggtgg tggaacggag atggcggaat aacgaccggg
    12901 tcgagattgt catgcccatg cggctgcgga ccgaggcgat gcccgacaat cccgaccgcg
    12961 tcgccctgct ctacggcccg gtcgtgctcg ccggtcaact tggcgatacc ttgcccgatc
    13021 cggtctatgg cacacccgtt ctgctgaccg ggaaaagaaa tcctgcggaa tggatccagc
    13081 ccgcgggggc tccgttcacc ttccgcaccg ccgggatcgg catgcccttt gatttcgatc
    13141 tcaggccttt ttacagcacg gcggatgact attacagcgt ctattttgac tattttactc
    13201 cggaggcgtg ggaagcgcga aaagaagcct atcaagccga gattcggcgg cagcaggaaa
    13261 tcgcgtcgcg gaccatcgat catttccgca tcggcgagat gcagcccgag cgcgaccaca
    13321 ggctggaggc gggggagcgt tcctacgttg accaggccct cgggcgctcc ggccgcgagg
    13381 cgcgcgccgg cagctggttc tcgttcgaga tggctgtccg gcctgaactc cccaacgcct
    13441 tgctgctcac cctcatcggc gatgatcgcg atcgcaaatt cgacctgctg gtggaagggc
    13501 aaaagattgc ggaaatcgac tggcggggag gggagaccgg ccgcttctac gatctgatct
    13561 atcccctgcc ggaggagctg ctgtgcggca aatcctccgt caccgtccgc atcgaggcca
    13621 accatggccg caccgccgga cggatcttcg ccctgaggac tatccggaac aacgcaccgc
    13681 tgcagccctg agtgcgatga cgggctgaaa aaaaacgccg ggccattccg cccggcgttt
    13741 gtgttaggag atagggcgca gtaactaaaa gatgaaatcg gtcgagagaa aacgcgatgt
    13801 gcgctcggtg acgatctccc ggatgatgcc ttgattggcg ctgttctcct tggcggcgac
    13861 cagggcgcgg atggtgaaga cgcgcagggc gtccgagacc gaaagagtgc cctcggcgga
    13921 atccttgcgt ccggtgaagg ggaagctgtc cggcccgcgc tgacactggc tgttgaggtt
    13981 gacgcggcag agctgattga ccagtggatc gatgaggccg gccagcactt tggcatcgtt
    14041 gccgaaaaga ctcacctgct ggccgtaatt ggagtccgaa atatagtgca gcggctcacc
    14101 gatctcgctg aaggaggcca ccggaatgag cggcccgaac tgctcctcgg tgtagagccg
    14161 catcccggcc gagaccggat agaggatcgc cggagtcatg catgtcccct gaactgatcc
    14221 gccgccgggg ttgatgaccc gcgctccccg ctcctccgca tcccggatat actcgtacag
    14281 ccgtccggtc ttgccctctt ccggcagggg ggtgatctgc acacccggct cccagggcat
    14341 gccgcacttt aaggccgcca ccgcggcgga gagtttctcc agaaagagat cgcaaacctc
    14401 cgcgtgcacc cagatcatct tcagggcagt gcagcgctgt ccgttgaagg agagggcgcc
    14461 gagtacgcac tctttcacgg ccagatcgag atcggcatcc ttgaggatga tggccggatt
    14521 tttggcgtcc aggccgagga cgcagcgcag acggtggggt ttggggtgcg cccggcggag
    14581 aatgtccgcc acccggctgg tgccgatgaa agcaaggacg tcgatgcggc cggattccat
    14641 caacggggtg atgatggtgg cgccgtcgcc atagatcgtg ttgatcaccc ccggcgggaa
    14701 acagtcgcga aaggccgcga gcaggggttc gaagagcagc acgcccagct tggggggttt
    14761 gaagatgacc ggattgccca tgatcagggc cggaatcagg gtggtaaagg tctcgttgag
    14821 gggatagtta aaggggccca tgcaaagcac cacgccgagc ggtgagcggc gaatctggcc
    14881 gatgacaccc tcttcgatga cgaagcggga ggagagccgg tcaagctcct tgagtgcgct
    14941 gatggtatcg cgtatatatt cgatggtgcg gtcgaattcc ttttccgcgt ccacctggtt
    15001 cttgccgatc tcccacatca gccgcttgac gatttcgccg cgctgctcct gcatgcgcgc
    15061 cgaaaaggcc gcgacgtggt ggatgcgctc ctccaccggc atggttggcc aggggccgcg
    15121 cccctggtcc caggcgcggg aggccgcttc gaggacctgc agcgactctg ctgcgctcat
    15181 caggggataa ctgccgatga cggcgcgctc cgctccggag ggaccgggat ggcagatcgg
    15241 cgacagaacc ggctgcgtca gcccggtcca ggtgcggagt tctccgccgc aaagccagct
    15301 gcgctggtcg acaggcacag ccagccgcca ggcttcgggc acagcttcag ggtcgggaaa
    15361 aatcgaagcg gggatgggct cattcatttg atcgctccgt gatctacctg gttttggcgg
    15421 tgaatttatt gcgcacaaag gccccggcat aggcgccggc gaaggcgatg agcagggcca
    15481 ggatcgccca gatattctgc accctgaaca agcccttgaa aaagccctga aaaaggacca
    15541 ggacgggtat ccagatgccc agggcaaggg cgaagagcca ggggtatttc gactggatga
    15601 ggccgaagag gaaagcgatg acaaagaggg cgatcgcgat gcccccgaaa tggatttccg
    15661 gccgggtggc gacataggtg atgatccccc ccaacgcgaa ggcaatgccc agcagaatct
    15721 tttgcatgat tttcctcctt gctttgatgt caggacacgc tctcctgccg cgctgccgga
    15781 tttttatcca gatggtggag caggccgcgg taggttttct ggaattcaac ataagcagga
    15841 aggttttcgc cgggctcgat ggtggtcagc tcgggctgca tcgcccgggc ggcctcctcc
    15901 ggcgtgctga agaagccggc cccagcgagg gcgaacatgg ctgcacccag caccgtggtc
    15961 tcgctcaccg ggctgaccag aacggggatg ccgagaatgt cggcgcggat gcggttccag
    16021 agatggtttt tcgagcctcc accggcgcag atgaccgctc cggccctgaa gccgccccat
    16081 tcctccagcc ggatcagact ttcacgggcc cgaaaggcga gggcctccag cgccgcccgg
    16141 taaagatgag ccctggtgga accggggaag aggccgctgt acaccccgcg cagcccgctg
    16201 ctgtcaaaca ggccggggag catgccaacc ccctcactgc ccggcgggag cagggaagct
    16261 tcgcccgtca tcttctcata acgctccgca cccccacagt cgctgtagag aagcccgccc
    16321 agccattcca ggatgcccga ggcgatccac atcatgcccg gattacacaa tcccggccgg
    16381 gcgtcgaatt cgattgtgat gcctgccgcc ggatccagcc cttccagatg gatgggggtt
    16441 gatcgggcca tcaggatctc ccaggtgccc gagctgagca ccggcagggt gggcccgccg
    16501 ccggcgccga agagggcgaa ctgggtgtca tggccggcga tcaccacggg tatcctcgcg
    16561 gcgaggccga attcggctgc ggcggcgggc agaagcgggc cggcaatggt gcccggctcg
    16621 gccagcgctg ggaacctgtc cgcatccagt cccgccaggg caagcagctc ctccgaaaag
    16681 gtgcggcggc tctggtcggt cagcatcgag gtcccggcca tggtgacatc ggtcaaccat
    16741 tccccggtga gtcggtggat gaagagggag ggcatcattg cgtaatagtc cattccctcc
    16801 agcaccccgg gccggttctc gcggaaccag agcagccgga aaagggtgtt gaaagggtag
    16861 gattgcagcc cggtgatgcg gaaaaggcgg agcggatcga tagaggaggc gatcttctcc
    16921 tggatggagc gggtgcggcc gcactgccag gagatgaccg ggtggagcag ggtgccgtcc
    16981 ttatggacag gggctccatc caccccgaag gtcgtcacgg tgatggcagc gatgcgggcc
    17041 tctggcgcgc gggaaaggag ctcacgggtg caggcgcaaa aacccgccca gatctcctcc
    17101 tggtcccaga tcaggcctcc cggccagccg ggatcagggt gaaccgcatt ggcgcggctg
    17161 gccgaggcaa gcagccgtcc cccggcatca accaaaatag cccgcacatt ggtggccccg
    17221 cagtcaagca cgagcgcggc tggttttctc gaactcatcg aagtgctgtg cctccatccg
    17281 gaaggcggcg ctgaatgccg cggccgggct gcactcatct cttgccgtaa aggggcccaa
    17341 aattcgcaca ggcgcgataa tcagccccct ctttgtccat gccgaaggcg ttccaggcgc
    17401 tggggcggaa gatctccgcc tcagcgacgt tgtgcatgca gaccgggatg cgcgccagcg
    17461 cggccagggt gatcaggtcg gcgccgatgt ggccgcagct gatcgcgccg tgattggcgc
    17521 cccagttgcc catcaccgca tagacatcgc tgaaggcgcc ctttccggtc aggcgcgggg
    17581 cgaaccaggt ggtcggccag gtcggatcgg tgcggtcgac cagggccttg ttgacctcgt
    17641 cgggcagatc cacactccag ccttcagcga tctgcagcac cgggccgagg ccctggacca
    17701 gattgacgcg cgacatggtc aggggcatgc ctccgcgggt gaggaactcc gaggagtagc
    17761 cgccgccgcg gaagtattca tagatggccg gcggccagag cgtggcggcg aggcaggcct
    17821 cgacctcggc gggggtgatt tcccagaagg gtttgaccgc cggttggcct gcaaccgact
    17881 ggcgcccgga aaagtccagg gcggaggcac cggaattgat gagatggatg accccgtgct
    17941 ccgcggcgcc gctcagggtg tggccggtga cgcgccggat cgccgccggg ctccagtagg
    18001 tgcgcacatc cgagaacatc tgggcggtgc tggtgagaag atggccgaag agcatggcga
    18061 ccccgttgag ggcatcattc tcggtcgcga ccagataggg ctgccgggtg ccattccagt
    18121 cgaaggagga gttgaggatc gcctccatga aatccccgtt gggggaatgg tcggtccatt
    18181 ggcgctggcc ctgaaagccg gcaagcaggg cattgtggcc gagggcctcc tcgccaaagc
    18241 cgagcgcggc caggcgggga ttccccgcca tcagatcgcg ggcgatgagg gtcatctgca
    18301 ccactttttc ccagatgcgc tgcttttcag ccggagtgcg tctggctttg ttgtggtccg
    18361 gcccttcaag gcatttggcg cgggtccagg cataggcggc gcggtactct tcgggatcga
    18421 agatcccctc ttccatgcgg cgggtgaact cggacatgtc gacgtattca ttgcgcatgc
    18481 ccagatagtt cctgaagaag gagggatcga ccatggagcc ggcgatgccc atcgaaaccg
    18541 aacccatcgc gagataagag gtgccgcgca tggcggcgat ggccaggcct gcgcgagcga
    18601 aacggagcag cttttcagcc acatcagcgg ggatggtctg attgtcggca tcctggacgt
    18661 cacggccata gatgccgaaa gcgggcagtc ccatctgggt atgggcggcg aggacggccg
    18721 cgagatagac cgctcccgga cgctcggtgc cgttgaaccc ccagaccgct ttggggcgaa
    18781 agggatccat atccatggtc tcggagccgt agcaccagca gggagtgacg gtgatggaga
    18841 cgccggcccc ttcgcgatca aagtaatccg cacagcgcgc ggcctcggcc accccgccga
    18901 tgcagctttc ggcgatgagg cactcgaccg gctcgcccga ggggtggcga agattttggc
    18961 tcaacagcgc ggcgaccgat ttcgccatcg ccatggtctg gttttcaagt gattcgcgca
    19021 cccccttgcg gcggccgtcg atggcggggc ggatgccgat ttttaccggc cggctttgat
    19081 gacgtgcatt catttcctga tcctgtcgat tatcagttcc gttaaacggg aggctctcca
    19141 agaggcctgg cggcacatgg agaagagtgg gcccgctcag actctcttcg ctgctccgtg
    19201 tcgcagtatg ggttgtttta tgtcaagctc aatcgatgcc ggcgcttttt tccgctgctt
    19261 cgacggtctg ggccatcagg gtggcgatgg tcatcgggcc gaccccgccg gggacggggg
    19321 tgtaggccga acagctctcc accacagccg caagttcgat gtcaccgatg ttgccggggt
    19381 gatagccggc gtcgatgacg accgcccccg gcttgatcca ctccccccgg atgaattcgg
    19441 gcttgccgac ggcgccgacg atgatatccg cccgccgcac cacctcggcc aggttctgcg
    19501 tgcgcgagtg gcagatggtc accgtcgcat gggcgttgag cagcatcatc gccaccggtt
    19561 tgccaaggat ggggctgcgc ccgaccacga ccgcctcctt gcccttgagg gggatctcgt
    19621 aatgcgcaag caaccgcatg atgccggccg gcgtggccga gccataggcg ggctcgccca
    19681 gggccatcct gccgaagccg agggccgtga ccccatccac atccttgctg agggcgatac
    19741 ggtcgaaaca ggcgcgctcg tcgatctgcg cggggaccgg atgctgcagg aggatgccgt
    19801 ggacatcggg attggcattg agggcgtcga tctcttccag aagccgctca gtggtcgtgc
    19861 tctggggcag gatcaccttg agcgattcca tgcccacgcg ctgacaggcg ttgcccttca
    19921 tgcgaacata agtggcggag gcgggatcgt cccccaccag aatggtcgcc aggatcggcg
    19981 tcttgccgga tatgtttttg atcttctcca cccgcaccgc caactcctgc tccatctgcc
    20041 tggccagggc cttcccgtcc agaatcattc cgctcatgac ttctcctcac aggtattatt
    20101 tgtttctgtc gtcattgaga tacccgggca catattcgcc gcgcttgatc ttgttggtcg
    20161 gggtcttgac gaagggggtc gactgttcga tcacccggct gatgcgggcg aaggcgggca
    20221 ggcgggtgtt gacctccctg cgcacctcct cgaggatctc gttgatcgcc cgggcgaccg
    20281 ccccctcggc gcgatccccc tcgatgctct cgatgtagct gtagttggga aaaatgcgcg
    20341 ccaccagttg tccttcggtc atgtagacca gggcctcttc aacgtaaatc gactcgagca
    20401 gcctggcttc gatggtctcg ggatagatat tctcgccgga ggggccgacg atgacgttct
    20461 tggagcggcc ggtgatgaag agaaagccat ccttgtccag atagccgcgg tcgccgctga
    20521 tgaaccagcc gtcccccgtg aagaccttgc gagtcgcctc ctcgttcttg taatagccct
    20581 tcatgacatt gggccccttg acgaggatct caccaatgcc ggtggtggga tcgggatcgg
    20641 cgatgtggat ggagacgcct gtgatggcat gccccggcga gcccatcttc tgctcctcca
    20701 tggaggaaaa ggtgaccaga ggggagcatt cggtcaggcc gtagccgcag cagtagggga
    20761 tacgcccctg cttcataaag gtctcgacct cgggcgaaaa ggcggctccg ccgatgataa
    20821 cgcattcgag gcggccgccc aggagcttgt tgaccttttt gccgatgatc ttgtagagca
    20881 gtttgcgcga ggcccggctt ttcgacagca tgcgcagcag gcggctggcg gcgatggtcg
    20941 gcaccacctg cttgtgatag atcttttcga agaccagggg aaccgccccg aagatggtcg
    21001 gcttgacctc ctgcatcgcc gccagcagca ccttgggggc cgggggcttg gtgagaaaat
    21061 agatcaggct cccctcgcgg ataatcgaga gaaagccgga ggtggagccg aaggtgtgag
    21121 cgagcgggag aatggagagg aggacgctcc gttcatgaat gcatttgatg aggtcggggc
    21181 cctcgaagag attgctcacc agattgcggt ggctgagcat cacccccttg ctgtggccgg
    21241 tggtgccgga ggtgtagatg atctccgcca ggtcctcctc gccgatcgct gccggcagct
    21301 ctggatccat tttggcgcgt ttgccgagca ccttgccgcc gaattcctgc aatcccttcc
    21361 agaagccggg cctggcgccc gcgccggaga cgagggccag atcggtgagg ctgaaaacaa
    21421 gaaatccctc gatgagagcg tgcaggttga ggctggcgtg aaggtggtcg gagataaaaa
    21481 tgacgctggc gtccgagtgg tggaggatgt gattgatgtc ggcttcgggg aaatcttcga
    21541 ggatggggac ggccaccgcg cccatggtgg tgacggccat aaaggcgatg caccagttgg
    21601 gcgaggagcc accgagaaga gcgaccttta ccccggattt gacgccgagc tcgcccagcc
    21661 tggcctgcag gtcggcgacc cgccggccga attcagcata ggtaaagggc tcatcgccga
    21721 ccagggccag ggccggccgc tgcggatagc ggttgagcac ctcggcgaag aagcagtcga
    21781 gcgtataacg ttccaggccg aatttgaggt tcggattgct cataaagatt cctccttggg
    21841 tgtgggcgga ccggccgcgc cgggtgccca atatacacat tctttggctc ttattcaaaa
    21901 ggcttttttc gcccgcgccg cacccaaaat tgtgacctgg cccgaaaaaa ggagaaaaag
    21961 tccttgtccg gcaagattat gttctttata atacagcaag agcaccccgc ttctctcagg
    22021 cgggtatccg gggagcatgt gagtgcgtgc agaagaaagg aatgagtatg cacaattcat
    22081 tgatgcgctg gcttggcctg gtgatgatcc tgatcgtcgc accggggatc gccgcgcagg
    22141 atctgcagga ttatctctcc cgctatctcg gcgataacgg aaaaaagtac ctccaacccc
    22201 tgggtgatgc ctttggcgcc aatctcaaca gcggcctcta ccacaccgcc catatcccgc
    22261 gtgtgggact gcatgtcggc gtcgaggtgg tcgcgaccgg agctctcatc tccgacgctc
    22321 agaggagctt caccgccacc accgaggagg gtttcgaacc gaagaagagc cttgaggtgc
    22381 ccaccgtttt tggcgatacc gcgccggtgg cggttcccgg tcagggtggc accgtctaca
    22441 actttccagg cggatatgac ctctcggtca tgcccctggc catgccccag atcaccatcg
    22501 gctccctcct gggcagcgag gtcacgctgc gctacgcggc ggccgagctc aatgaggagg
    22561 tcggggatct cagcctgctc ggcatcggct tccgccacag cctcagccag tacattccgc
    22621 tctttccggt cgacgtggcg gctgcggtct tttatcagac cctgaccgtg ggggatatcc
    22681 ttgacgccaa aacccgcgtc atcggcctct cggccagcaa aaccttcaac cttttcaccc
    22741 tctacggcgg cctctcccat gagaactcgg tgatggaggc ctcttatgaa cacggggagg
    22801 gcgaagaggc cgaaaccatc tccttcgatc tggagggggc gaacagcgtg cgcctgaccc
    22861 tgggggcgac cctcaagctg gccattttcc agcttcacgc ggactatagc ttcgccgccc
    22921 agcagagcgc ctgcctgggg ctctatatcg gcctctaatc actgcccacg atccttaatg
    22981 actccgggag gagcaatgaa taaaagaatc ggacttgtcc tgctggccgg cgccgcgctc
    23041 ctgatgagcg gctgccagaa aaagctcttt catttcgtcg tacccctgaa caaggtcaaa
    23101 accattgcga ttaataatac gggcgaattc gccggctatg agaaaataac cgccagggac
    23161 attcgtgcag ccctggatgt gccggaagac ggcaccatca ccggtgtgga ccttgaatcg
    23221 gtgcaaatca gggtgcggct cgtcgagggc aacgtggcga cctgggtcaa actctccgga
    23281 tttgtccttg accagggcaa accagtgcag ctgtttgagg acaaatcggt ccctctcagc
    23341 ggtgtggata ccaaatatat cggcttgaat gccctgatcg caagaggcgt ggaaaaactg
    23401 gccggcaaga ttaacgatca cgtcaaaaaa gtgaatgatg ctgatatcga gatcggcctg
    23461 accggcaaca gcgacccggc tggccagctc atcaaggtgg agatcgattt caagatcaac
    23521 gccactgtca aatacgacca gtgcgtcgag gttttcgatt tcatcggcgg agaggattgc
    23581 accgaatagg gactgcctga accagggaga gccgggacac ggggagggag ccccggcctc
    23641 tctccgcatc aacggatgat gccgacgggt atcctccgtt ttttaatgcc ctgccgccaa
    23701 attgttgggg gcagggcaac aaattgcttg acattggcct cttttctgct aaattatcca
    23761 tgtaaacggt tacaattcat gggctcccgg aggccggatg acgcaccgtt gcacccccct
    23821 gctcgcctgc attctcgcct gctgcctgct gaccggctgc cgccgcgatc ccggcgaaca
    23881 gctcctgctc gatttctggg ccatgggcgc cgagggcgaa aacatccagc ccctcctgcc
    23941 cggatttgct cggcgccatc ccgaaatcac cgtccgggtc cagactgtcc cctggggggc
    24001 cgcccatgac ctcctcctca ccgccttcgc cggcggttcc agccccgatc tctgccagct
    24061 cggcaatacc tggatccccg aattcgcggc catcggcgcc atccggccgc tcgatccctt
    24121 tttagccgat tccaccctca gaccaggaga ctacttcccc ggcatctggg agaccaactg
    24181 cattggcggc cgggtctatg gcatcccctg gtacgtcgac acccgcctgc tcttttaccg
    24241 cagcgacctc ctcgccgcag ccggctaccc ccggccgccg cgcgactggg gggagtggct
    24301 cgatctcagc cggcgtctgg tgcagcagca gcccggccgt tacgccctct ttctttcgac
    24361 gattttcaat gactggcagg tgccggccat cctcatcctc cagaacggcg gccgactgct
    24421 gcgcgagaac gactgctacg gcgccttttc cgaaccggcc accctcgcgg ccctgcgcta
    24481 ctatatcacc ttttttcacg actccctggc cgtgatcaat atgagcgagg tggccaatat
    24541 ttttcagggc ttcagcgagg
//
LOCUS       DAOPWU010000013        23966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_2089_length_23966_cov_5.550249, whole genome shotgun sequence.
ACCESSION   DAOPWU010000013 DAOPWU010000000
VERSION     DAOPWU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23966
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2089_length_23966_cov_5.550249"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1885)
                     /locus_tag="PKM23_01710"
     CDS             complement(32..1885)
                     /locus_tag="PKM23_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY90217.1"
                     /translation="MQRDYQILIIEDHPEQAHLMELILRRHGQPFSVRIIHDPEEGLA
                     WLKHNPVDALVLDYSLPKMSGLEVLQLLKKEYPGLPVIMVTGQGDERIAVEAMRHGVY
                     DYLVKTKDFLDLLPRVVTRAIEEQQLSSRLEQSEQRYFALFDKAGIAIFIADADSYRL
                     YQANGMALKLLGQPVEALLQKSFINLCVSRSLPEVEEFLARIKSEGQANLDQVMLVHA
                     DRRLIPVDLTGSLVSVGNHQVIQCFARDISEKLKMQRQLLLSRQRLISLFDGITDPIS
                     VQDRDHNLIMGNKRYMQLTAANTPGLVGCKCHLALFGSDQPCANCPAFETYRTGESRF
                     IEIFHQGRTYHIWTFPMAGLDGKPEFLVEYAKDVTDQKEIEKQLIKSEKLATIGLLSS
                     GIAHELRNPLNIIETARYSIEDGLGHTQPEIDRKLEIIKKNIRRASVIIENLLQFSRH
                     SVHEREPVDVEKLIDTTLSLVEKETSVRNIILEKGYGGVPRVFFSLDSLKQVFLNIIY
                     NAVQAMPSGGVLRIITTASSDEKWVNVEFTDSGVGISPENLSHIFTPFFSTKRESGGT
                     GLGLYLSYTIIKREGGDILARSREGSGTTFTVRLPMVKSSGAPEPAELAVD"
     gene            complement(1890..3452)
                     /locus_tag="PKM23_01715"
     CDS             complement(1890..3452)
                     /locus_tag="PKM23_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY90218.1"
                     /translation="MSHTDYENIDRFYALAANASLISDPAEIFRQARPLLLGMIPADY
                     FLIFFNDKLRECLSASFAYNILPLPTIEEVTIPYQDLLISDLLISRQTVSRQDHIDPL
                     VLDVSQELFVPILTPEEVPGALYFARMTEEPFSAEEIALAEFCAYLLVAPMERAHWEE
                     RSQRSHEILNTFREKYLAILDALPYPAVVIDPAEDRLEEVNRAFLEWTGYDRRQIFMC
                     RFSDLCRTPLAFCQTAAWPSGPEKAQVADAKGALHEVQAFHSWLTSREPEKRIVTFVS
                     DWRPARGEEPQPDAELLIRTLAHDLKAPLQSLKGYATLLWEEQGYHLPAGAVTYLERI
                     SANIEKMEHLIADLLDLSRLNGGEVPFQEADSFEILKNALDSLAGLIEKRPVNMIIDS
                     TLPRITCNSVQMTQVFTNLISNALKFTHGVEIPSVEVGCTAVEDDYEFYVKDNGVGIA
                     PADLEHIFDLFYSRDADEKNRSTGVGLTIVKRIIEAHHGRIWAESTPGKGTVIRFTLP
                     QLLSRQVTFAGE"
     gene            complement(3473..4465)
                     /locus_tag="PKM23_01720"
     CDS             complement(3473..4465)
                     /locus_tag="PKM23_01720"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNY90219.1"
                     /translation="MIHTREWQTVRDFIQDRTRIAVTTHVNPDGDAIGSQMAMAHYLR
                     QIGKEVVLLNCDRMPDYFSFLDPEGEIALFEEQAHDALVAGLDGCIVVDISDWARLRG
                     LGSALRRHNIPVACIDHHIPSDKMGEVHVCYQEASSTGEMIYDFLLDSGAELTLPIVD
                     AIYTCVMTDTGSFRFSNTNARTHRIAAELLERGAHYRAIYEKVYENNSKQRTLLMAKL
                     LERMHFACDDRLAYFSLTQELLRQTGAQLWETEGFSELPRTIARVEVSLMFTETEAGN
                     TKVSFRSKGRVAINGMATLFGGGGHKYASGATLAMSLNQAVEAVVEEARKLFTD"
     gene            complement(4462..6558)
                     /locus_tag="PKM23_01725"
     CDS             complement(4462..6558)
                     /locus_tag="PKM23_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90220.1"
                     /translation="MLLFSLPAARADAAGPALRTWQSFADFITGRLDNLSLSADGRLT
                     LAPSTTQIVDTGEPYVWDIASDAAGNLYLGTGNGGKVFRITPAGDTTLYFQAEEPEIY
                     ALAHDGRRSLYVATSPNGFIYRIDDGGKAALFCEPGQSYIWDLLCDPRGDLWAATGGK
                     ARLLRISPDGRMSVVFSSEAEHLRCLALDGEELFAGSSRPGLVYRIRKGQAPFVLYDS
                     GSEEVHSLAVMPGGHLYAATMTPAGPEAAITRILAAAPAQSVSPEEAAEEKSGALQQP
                     PLAGGIESAPAAKSQLLRIEADGYGRPIWPAGLDEVQALLPGPDGKLLVGTGREGRLY
                     SIDSAGEISLLLKIGAAQIPTLHRTSAGEVILATANMGLACRVGPRVREGSFESETVD
                     AAALTRWGALRWRGEGRMTFQTRSGNTRKPEASWSEWQPLQSSGGSWQIASPAARFLQ
                     WRCTLQGGKEEPALAEVNLAWLQQNRPPEIADLVVLPPGSYFEITPDPAAGTTGLSAP
                     APLPKRDQRKGWRSALWQFDDSNSDPLLFALWYRRTGDAHWRRLAGPLTQAAYSWDAT
                     QMADGEYELKLEASDSLMLPAGQGLRAEKISPPFVVDNTGPVIKGFEVENKDGIRHIR
                     FSVCDAASPLARVRISINAGGWKPLSPVDGICDSVCEEFGFTLRSGEELLELSLEAAD
                     IFENSSMAHQRIKGSR"
     gene            complement(6603..8387)
                     /locus_tag="PKM23_01730"
     CDS             complement(6603..8387)
                     /locus_tag="PKM23_01730"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HNY90221.1"
                     /translation="MKPGMTGVGRSVFQGEEIDTFGVVVVDVAPNFYPGEDVIIVRLT
                     GPRAEQAGVVAGMSGSPIYLQGRLAGALAMRFLEFQKEPYAGVMPIASMMRASDYETR
                     RGTERPCAPQSGQHYLRAVLLGAEGDFWQRMAAPLLPASPAGMAMRRIETPLLFSGFS
                     EQTLSASAELWSSLGFAPMMGGESVQEGSSGAPLEPGSAISQLFIRGDFSMDMTGTVT
                     AVEGDQLLAFGHYLFNLGPVQMPMARSRILATLPSLMGSSKLARSLEVIGTIRQDRLS
                     GVYGRVGVMPAWIPVQVELTGPAGRRSFHFEMADDPALRNLTPFFLRSALFQALVAGK
                     LGAEPSTVRLSCNIDLKDGRRITVGDYISYQERLGFLGAGSEIAEAVDLVTMSIGALT
                     VNTFDPPPLRAVEVTAQVEPGERVAAIRSIRQDRLEVSPGDSLHLTMTLQRTSGQEIR
                     FTQCVVLPRYLQSSTLTVIAGGAAGLIQAEMQSNPDKYRPDSFSQLCALLEERRTSDN
                     LYLQIREPARGMVVDGEELTGLPPSILRTMNGRGSERILRDRVLAEWAIPADCEITGL
                     KRMTVRITQPRTNWPDDVQDGTEQLFLE"
     gene            complement(8562..9281)
                     /locus_tag="PKM23_01735"
     CDS             complement(8562..9281)
                     /locus_tag="PKM23_01735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNY90222.1"
                     /translation="MSQLDLKGRIVWVTGSGRGIGRAIALSLAAHGAHLVLSARTISD
                     IEAVAQEITNQGGPAVAMPCDVRHPDQVKRVVVAVERTHGPIEVLVNNAGVAIFGKIV
                     DTSESDWDLMMETNVKGAFLCSQAVLPGMIERKSGHIINMVSVAGRTPYYNNAGYCAS
                     KYGLLGFTDVLRMEARKHGIRVTSLMPGATDTLIWGDADVDNTRMMPADEIAQAVTAI
                     LLTGESAMMEEVVIRPLGGDL"
     gene            complement(9283..9843)
                     /gene="folE"
                     /locus_tag="PKM23_01740"
     CDS             complement(9283..9843)
                     /gene="folE"
                     /locus_tag="PKM23_01740"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNY90223.1"
                     /translation="MAKSLEPLVREMLAELGEDPQREGLLRTPKRVDAALRYLTHGYS
                     QSVEEILNDAIFTEECEEMVVVRNIEFYSMCEHHMLPFYGRAHVAYLPKGKIIGLSKV
                     PRLVDMFARRLQVQERLTNQIAGTLMEVLKPYGVAVVMEGKHLCMMMRGVEKQNSFVT
                     SSAMLGEFKKDRATRMEFLNLIKSWE"
     gene            complement(9879..10295)
                     /locus_tag="PKM23_01745"
     CDS             complement(9879..10295)
                     /locus_tag="PKM23_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNY90224.1"
                     /translation="MPLVYVTRLLHFNAAHRLHSDLLSSEENQSIFGACNNPLGHGHN
                     YDLEVTVAGEPDPVTGMVIDLKVLKEIVEEAVISRVDHKHLNFDVDFMQGIVPTAENI
                     AIAFWEQLKDRFTGCELYELKLWETPRNMAVYRGGR"
     gene            complement(10445..11542)
                     /locus_tag="PKM23_01750"
     CDS             complement(10445..11542)
                     /locus_tag="PKM23_01750"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNY90225.1"
                     /translation="MMPSSGSIPICDLHCDTATNLLLGSPLTDRGMQVNLPGMIEGGI
                     GLQVFACYIPPAIPRGQAFPMVEKMIVNLKAELARHPEIALCTNLAEIGSARASNRIA
                     AFIGVENGNAIDGDLANLEKLRDLGVRLMTIIHAESNEWAISSNDPAPRFDGLTDLGR
                     DVIQAMDDLGIIIDVSHSHDRTVEEVLRHSRKPVIASHSCAKTLCPNARNLSDELIAG
                     LAYSGGVVGLNLYPGFLDAGYSRRITERAGDLFSELGKMERAAGMDIVKIGELYPGFV
                     ARFTEAMGDDLLPLERYYDHILYVTELIGAEYVAFGSDFDGVPILPEGVAGCASMRRI
                     QEGLYERDYSLDEIQAISWDNFMRVIGAVCG"
     gene            complement(11687..12133)
                     /gene="mscL"
                     /locus_tag="PKM23_01755"
     CDS             complement(11687..12133)
                     /gene="mscL"
                     /locus_tag="PKM23_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNY90226.1"
                     /translation="MFKEFKEFAMRGNVVDMAVGIIIGAAFGSIVSSLVADVIMPPIG
                     MLLGNVDFSNLFVVLKEGAAAGPYASLAAAKSAGAVTINYGVFVNAVINFLIVALAIF
                     FLIRGLNRLKRKEEAPAEVTTKECPQCLSTIPLKARRCPNCTSQLE"
     gene            complement(12195..12695)
                     /locus_tag="PKM23_01760"
     CDS             complement(12195..12695)
                     /locus_tag="PKM23_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90227.1"
                     /translation="MLVWVVYESRLGNTAALAQAIAGALAREHRVRLLPVEEAGPPSG
                     VDLLIIGCPHHRHHQPDGILSWLFKLPPRVVQDLNCGVFEIRYKRHHFWQGESAATLI
                     WRRLQGLGGKPMLKPVSFYLQHRGRLLPESEIERGAAWAAALARKAVGGRVKAPVIHA
                     GDHIWT"
     gene            complement(12820..13947)
                     /locus_tag="PKM23_01765"
     CDS             complement(12820..13947)
                     /locus_tag="PKM23_01765"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNY90228.1"
                     /translation="MFLFWAGAALHAQEYVTVSPGKSDGVYTLLKRYGLPPSKVMIAR
                     FWELNSDLVRGGEYLRLGFEYKLPVYKLYYDGASIRSSLGISDLALARRIEAYNHSLY
                     EAGVLAAPFKKSRELLVPAYWLSESSAAAEAASFPIFGPAYQSVKQKDQSLKGCVIYL
                     DPGHGGPDPGAIGQRGRDRLYEDEYAYDITLRLARRLLEHGASVYMVVQDEDDGIRDL
                     AILKHDDHEYYLGRLPISRDHLRRLSDRVDIVNNLYERHRKKARQQIMVTIHLDSRSH
                     GKRIDIFYYYQANNPKSKKLANTLLAKVDEKYAAKQPGRGYEGSVSTRDLHVLSEAKP
                     VAVYIELGNIRNKKDQDRFIIADNRQAVANWLCEGVIDYVK"
     gene            complement(14005..14829)
                     /locus_tag="PKM23_01770"
     CDS             complement(14005..14829)
                     /locus_tag="PKM23_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNY90229.1"
                     /translation="MKKNDKSMDKYRILPGKPVDLKKLDPNDSSNFDGDKKEGKEELL
                     KLNKKLEGLQELLYAEQKRKFLVVLQAMDTGGKDGVIRHVFEGVNPQGVRVASFKTPT
                     AIELAHDYLWRIHQQAPARGEIVIFNRSHYEDVLVVRVHELVPREVWSRRYDQINAFE
                     KQLTEEGTTILKFFLHIDKDEQKARLEARRDDPEKRWKFNVDDLKERKLWDEYVRAYE
                     EAISRTSTEWAPWYVVPANKKWYRNLVVASAIIDKLEGLKMAFPEADFDPKKVKVD"
     gene            complement(14893..16824)
                     /locus_tag="PKM23_01775"
     CDS             complement(14893..16824)
                     /locus_tag="PKM23_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNY90230.1"
                     /translation="MMSKIARTAKPVQVSVSPYRPIRLHPVQPVPSDIAIAQAARMKK
                     INSLAREAGILPEELEPYGDYKAKVRLSILDRLADRPNGKYIVVTATTPTPLGEGKTT
                     TTLGLSQALGAHLKQRVFTCIRQPSQGPTFSIKGGAAGGGYSQIVPMEDFNLHLTGDI
                     HAITAATNLLAAAIDARVLHESKRSDDEQLARLLCKKGEFAISQRRRMERLGIKAQRL
                     ADLSLEEMRRLFRLDIDPEHITWKRVVDVNDRLLRRIRIGLGDEEKGQERETGYDIAV
                     ASEVMAVLALTTGLADLRERLGRMVVARSRSSEAVTAEDIGAAGAMTVLLKDAIMPNL
                     MQTLEGSPAFVHAGPFANIAHGNSSIIADRIALKLSDYVITEAGFGADIGLEKFVDIK
                     CRYSGLKPDAAVIVASIRALKMHGGGPTVVAGKPLDPVYSESNPELVEKGCSNLAKAI
                     DIARQFGIPALVAINTFPGDTPEEIAVVKRAALAAGAHGAYVCNHYALGGAGIIELAE
                     AVMDATGQKNNFRFLYPPEWPIKDKIHAIATSIYGADGVDYSPEVESAIDYYTRLGLG
                     SLPICMAKTHLSLSHDPALKGVPTGWRLPVTEMKASVGAGFLYPLCGQMRTMPGLPTR
                     PGFLDVDIDTESGKVIGLS"
     gene            17015..17326
                     /locus_tag="PKM23_01780"
     CDS             17015..17326
                     /locus_tag="PKM23_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HNY90231.1"
                     /translation="MSTLTGYQRRYLRGLANPLKPCIQIGKNGLTGQVIEAIDAALAD
                     HELIKVRFLELKEERKQISAEIAGRTGCELAGLIGHVAIFYRCSTDPAKRKIQLPVKS
                     A"
     gene            17445..18794
                     /locus_tag="PKM23_01785"
     CDS             17445..18794
                     /locus_tag="PKM23_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNY90232.1"
                     /translation="MTLDIIVLLALILANGVLAMSEIAVVSVRKTRLQQLANEGHKRA
                     QSVLDLTNNPSRFLSTVQVGITLVGIMAGAFGGARFTEGLAQWLGKFEAIASYAHPLA
                     FAIVVLIITYLTLILGELVPKQIALHNPEKTAGALTPTMQRLAWIARPAVAILSYSTD
                     TVLRLFGVSKVAEQPVSEEEIKVMIDQGIEAGMFEEAERDMIESVFSLADKRAGALMT
                     PRTDVVWLSTQDAPVETYKKILESEHSRFPVCEGSLDEVIGVVRAKDLLENAISSREI
                     DLRKHMQPALYVPESAMALNVLETFKKQRQHIAIVVDEYGVMQGIVTIFDILEAIVGD
                     IPSMDETDDRPGVQKRLDGSYLIDGLYYMEEFKELFEIDEDLPEESSYQTLGGFVLMH
                     LGRIPVATDFFEWSKWRFEVMDMDGTRVDKVLVTPLNIQEPDAAVNELQQMNNPKER"
     gene            18794..19654
                     /locus_tag="PKM23_01790"
     CDS             18794..19654
                     /locus_tag="PKM23_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNY90233.1"
                     /translation="MHYRTIELIGRSVPTLEGAGVRLQRVFGFGQAPNLDPFLLLDHF
                     GSKNPEDYMAGFPWHPHRGIETVTYLLTGAVDHGDSLGNKGIIGSGEVQWMTAGSGIV
                     HQEMPRHVDEPLNGFQLWVNLPAAQKMMIPRYNGILSSQIPAVELEPGIQAKVISGSL
                     AGAQGPMKDLVVDIGYYDLTLAPGSRIGLPLPAGHRTFAYLYDGRGSFAAAGDQLAGA
                     GQLIVFADDGEAVQIQAGEEGMRFLLVHGQPIGEPVAWRGPIVMNTDEELRTAFAEFR
                     AGTFIKHKPA"
     gene            19666..21852
                     /locus_tag="PKM23_01795"
     CDS             19666..21852
                     /locus_tag="PKM23_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNY90234.1"
                     /translation="MKTISALRLIPLVLTLSLAAALGQNRSIELHRASGPIIIDGFIE
                     PAWANADSAGDFFQLQPYYARPPLHRTIAKLLTDDRNLYCLILCQDEPGHIQRNTGKH
                     DDFSGDIVSLMLDTFSDRRTAYKFAVNAGGSRADCRLLDDARNRDYSWDGVWFSASRI
                     HDWGWAVEMEIPYRSIQYGEGLDEWGLDFDRWSATEQEDTYWNPYDQAEGQRISKFGR
                     LLFTDYHPAVHVINLEIYPVGLTKAEQERGDGWSFSPNAGVDLFYNPSQRLTFQLTGN
                     PDFAQIEADPFEFNISRYETYYSERRPFFTEGNEVFMPSGKQRESGFYSPMELFYSRR
                     IGRKLPDGSEVPLLVGSKAFGRVNSWEYGGFIAATGREEFQEGTASGSEPAALYSSVR
                     LKKQVMGNSSVGMLYVGRNSGDENNGVVDLDGAFRASDWQLSYQIARSYRNDEGDYAA
                     SVGLWMPKTSWILGLRSRYVGEKFDIQEVGFVPWRGTWNGALLTGPVWRLQTGAVSQI
                     MVYGGGLFNHEKVDGYADRMAALGYNMQFRKNWGFEIGVTAGRSRDMGVLYNNREATL
                     SSWFNLSPVWEGNLYGGYSRTYNFSRGYLASYGWAGGEIDWKAARILALGSSADIFFE
                     GNPAGAVEEITFNARPYLSWTPVNNLNMRLYVDNLYLRTTGHLERVIGGFLFSYNFRP
                     KSWLYFALNEVQDRSNEYDHEEQLLPNRLHMVNRVNVVKLKYLFYF"
     gene            complement(21939..>23966)
                     /locus_tag="PKM23_01800"
     CDS             complement(21939..>23966)
                     /locus_tag="PKM23_01800"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNY90235.1"
                     /translation="GIRYEFGIFDQEIREGWQREIADTWLRFGNPWEIARAKWCQKVG
                     LGGRTESWTDENGRYRVKWIPARYVKGLPFDTPIIGYANQKCNTMRLWKAEAIVSFDF
                     ADFNQGDYYAAVEDKISSETITKVLYPNDETETGKQLRLIQQYFFVSCSLQDMIRIHL
                     SRHANLDNFPDHFAVQMNDTHPSIAVAELMRLLVDVHRMEWERAWQITRSTFCYTNHT
                     LLPEALERWPLKLFGDLLPRHLEIIFEINRRFLDEVRERHPGEEKRAERLSLIDEAGE
                     RSLRMAHLACVGSHAVNGVARMHSDLLKRTVLHDFYELWPERFFNVTNGVTPRRWMAL
                     CNPGLSGLISSRIGSGWIRDLEQLRQLEAFAGDAEFGEEWRRVKLENKKRLAERIRKV
                     TGIEISPDSLFDIQAKRIHEYKRQHLNLLHIIALYLRMKEDPGAEVVPRTCIFAGKAA
                     PGYFMAKLIIKLTTAVAEVVNADPDVNGRLKVVFFPDFNVKNAHYIHPAADLSEQIST
                     AGKEASGTGNIKFALNGALTIGTLDGANVEIREEVGEENFFLFGLTAGEVLGMKAAGY
                     NPAAFYDSNPLLRQVIDLIRSGRFSPDQPDLFRPLTDCLLNRDEYMLCADFDAYVACQ
                     TRAGEAYHDREGWTRRSILNTARMGKFSSDRSIRDYCRQIWQIMPPPPAGA"
BASE COUNT         4581 a         7326 c         7420 g         4639 t
ORIGIN      
        1 tttgatagat aatgtaataa taatagttaa attagtcaac agctaactca gcaggttccg
       61 gcgccccgga gcttttcacc atcggcagcc ggacggtgaa agtggtgccg ctgccctcac
      121 ggctccgggc gagtatgtcc cccccttcgc gcttgatgat cgtgtaggag aggtagagcc
      181 ccaggccggt ccccccgctc tcccgcttgg tcgagaagaa gggggtgaag atgtggctga
      241 gattttcggg ggagatgccg acgccgctgt cggtgaactc gacattgacc cacttttcat
      301 ccgaactggc cgtggtgatg atgcgcagca ccccgccgct gggcatcgcc tgcacagcgt
      361 tgtagataat gttcaggaag acctgcttga gtgagtcgag gctgaaaaag acccggggaa
      421 ccccgccgta gcccttttcc aggatgatat tgcgcacgga ggtctctttc tcgaccaaag
      481 agagggtggt atcgatcagt ttttcgacgt cgacgggttc ccgttcgtgc acggaatggc
      541 gggagaactg gagcaggttc tcgatgatca ctgaggcgcg gcggatattc ttcttgatga
      601 tctccagctt gcggtcgatc tccggctggg tgtggccaag cccgtcctcg atggaatagc
      661 gggcagtctc gatgatattg agcggattgc gcagctcgtg ggcgatcccc gaggagagca
      721 ggccgatggt ggccagcttc tccgacttga tgagctgctt ctcgatctcc ttctggtcgg
      781 tgacatcctt ggcgtattcg accagaaatt ccggcttgcc gtcgaggccc gccatcggga
      841 aggtccagat gtggtaggtg cggccctgat gaaagatctc gatgaaacgg gattcgccgg
      901 tgcggtaggt ctcgaaggcc ggacagtttg cgcagggctg gtcgctgcca aagagggcca
      961 gatggcattt gcagcccacc agccccgggg tgttggccgc cgtgagctgc atatagcgct
     1021 tgttgcccat gatgagattg tggtcgcggt cctgcaccga aatcggatcg gtgatgccgt
     1081 cgaagaggga gatcagccgc tgccgactga gaaggagctg ccgctgcatc ttgagctttt
     1141 cgctgatatc ccgggcaaaa cactggatga cctggtgatt tccgaccgaa accaggctgc
     1201 cggtgagatc aacagggatg agacggcggt ccgcatgcac cagcatcacc tgatcgaggt
     1261 tggcctgccc ctcgcttttg atgcgcgcga gaaactcctc gacctccggc agcgagcggc
     1321 tgacgcagag gttgataaag ctcttttgca gcagcgcctc gaccggctgt ccgagcagct
     1381 tcagagccat gccattggcc tgatagaggc gatagctgtc ggcatcggcg atgaaaatgg
     1441 cgattcccgc cttgtcgaag agggcgaaat agcgttgttc gctctgctcc aggcgggagg
     1501 agagctgctg ctcctcgatc gctcgcgtca ccacccgcgg cagcagatca agaaaatcct
     1561 tggtcttgac cagatagtca tagacgccgt ggcgcatggc ctcgaccgcg atgcgttcat
     1621 ccccctggcc ggtgaccatg atcaccggca gtccgggata ctccttcttg agcagctgca
     1681 gcacctcgag tccggacatc ttgggaaggc tgtaatcaag gacgagggcg tcgacgggat
     1741 tatgcttcag ccaggccaga ccctcttccg gatcgtggat gatcctgacg gagaagggct
     1801 ggccatggcg ccgcaggatc agctccatca ggtgggcctg ttccggatgg tcttcgatga
     1861 tcaggatctg gtaatcacgc tgcatcgtat cactccccgg cgaaggtcac ctgccggctc
     1921 aagagctggg gaagggtgaa acggatgacc gtccccttgc ccggcgtgga ttcggcccag
     1981 atgcggccgt ggtgggcttc aatgatgcgc ttgacaatag tcaggccgac cccggtgctg
     2041 cggttttttt catcggcatc acgggagtag aaaaggtcaa agatgtgctc cagatccgct
     2101 ggggcgatgc cgacaccgtt gtccttgacg taaaactcgt aatcatcctc gacggcggtg
     2161 cagccaacct cgacgctggg gatttccacc ccgtgcgtga atttgagggc attggagata
     2221 agattggtga aaacctgggt catctgcacc gagttgcagg tgatgcgcgg cagggtggaa
     2281 tcgatgatca tgttcaccgg cctcttctca atcagccccg caagggaatc gagggcattc
     2341 ttgaggatct cgaaggaatc ggcctcctgg aagggcacct ccccgccgtt caggcgggag
     2401 aggtcgagga gatcggcgat gagatgctcc atcttttcga tattggcgga gatgcgctcc
     2461 aggtaggtga cggctccggc ggggagatgg tatccctgtt cctcccagag cagggtggcg
     2521 tagcccttca ggctctgcag cggggccttg agatcgtggg cgagggtgcg gatgagcagt
     2581 tcggcatccg gctgcggctc ctctccgcgc gcagggcgcc agtcgctgac gaaggtgacg
     2641 atgcgctttt ccggctcccg ggaagtgagc caggagtgaa aggcctggac ctcgtggagg
     2701 gcgcctttcg catcggccac ctgcgccttt tcagggccgg agggccaggc tgcggtctgg
     2761 cagaaggcca gcggggtgcg gcagagatcg gaaaagcggc acatgaagat ttgccggcgg
     2821 tcgtagccgg tccactccag aaaggcgcgg ttgacctctt cgagacggtc ctcggcggga
     2881 tcgattacga cggccggata gggcagggca tcgaggatcg cgagatattt ttcccgaaag
     2941 gtattgagga tttcgtggct gcgctgggaa cgctcctccc agtgggctcg ctccataggg
     3001 gcgaccagga gataggcgca gaactcggcc agggcgatct cttcggcaga gaagggctcc
     3061 tccgtcatgc gcgcgaaata gagcgcaccc ggcacctcct ccggggtgag gatggggaca
     3121 aagagctcct ggctgacatc gaggacgagg ggatcgatat gatcctggcg gctgacggtc
     3181 tgccggctga tcaggagatc actgatgagg agatcctgat aggggatcgt cacctcctcg
     3241 atggtcggca ggggcaggat gttataggcg aaggaggccg acaggcattc gcgcagcttg
     3301 tcgttaaaga agatcagaaa atagtcggcg gggatcatgc ccagcagcag gggccgggcc
     3361 tggcggaaga tttccgccgg gtcggagatc agggatgcat tcgccgcaag ggcatagaat
     3421 cgatcaatat tttcataatc cgtatgggac atgcggaggt ttgattcgcg tttcagtccg
     3481 tgaacagctt cctggcctct tccactaccg cctccactgc ctggttcaga ctcatggcca
     3541 gggttgcccc ggaggcgtat ttgtgtccgc ctccgccgaa gagggtcgcc atgccattga
     3601 tggcgacgcg tcccttggag cggaaactca ccttggtatt gcctgcttcg gtttccgtga
     3661 acatcagact gacctccacc ctggcgatgg tgcgcggaag ttccgaaaag ccctcggtct
     3721 cccagagttg ggcgccggtc tgacgcagca gttcctgcgt caggctgaaa taggccagcc
     3781 ggtcatcgca ggcgaagtgc atccgctcca aaagtttggc catgagaagg gtgcgctgct
     3841 ttgaattgtt ttcgtaaacc ttttcgtaga tcgcccgata atgcgcacct cgttcaagca
     3901 gttccgcggc gatgcggtgg gtgcgcgcat tggtattgct gaaccggaag gatccggtat
     3961 cggtcatgac gcaggtgtat atggcatcga cgatcgggag cgtcagttcc gctccggaat
     4021 cgaggagaaa atcgtagatc atctcgccgg tggaggaggc ctcctggtag cagacatgga
     4081 cctcgcccat cttgtcgctc gggatgtgat gatcgatgca ggcgacagga atgttgtggc
     4141 ggcgcagggc gctgcccagg ccgcgcaggc gcgcccaatc gctgatatcg acgacgatgc
     4201 agccgtcgag cccggcgacc agggcatcgt gcgcctgttc ctcaaagagg gcgatttcgc
     4261 cctccggatc gagaaaagaa aagtaatccg gcatacggtc gcagttgagg agcacgacct
     4321 ccttgccgat ctggcgcaga tagtgcgcca tggccatctg gctgccgatg gcgtcgccat
     4381 cgggattgac atgggtggtg accgctatcc tcgtcctgtc ctgaataaaa tcgcgaaccg
     4441 tctgccactc tcgggtatgg atcatctgga accttttata cgctgatggg ccatgctgct
     4501 gttctcaaag atatccgcag cctccagtga gagctccagg agctcctccc cgctccggag
     4561 ggtgaagccg aactcctcgc acacgctgtc gcagatgcca tccacggggc tgaggggttt
     4621 ccagccgccg gcgttgatgc tgatgcgcac ccgggcgagg ggtgaggcgg catcgcagac
     4681 cgagaaacgg atgtggcgga tcccatcctt gttctccact tcgaacccct tgatcacggg
     4741 gccggtgttg tcgacgacga aaggggggct gattttttcg gcgcgcagtc cctggccggc
     4801 gggaagcatc aggctgtcac tggcctccag cttgagttca tattcgccgt cggccatctg
     4861 ggtggcatcc caggaatagg ccgcctgggt gaggggtccg gccagccggc gccagtgggc
     4921 atcacctgtg cggcgatacc agagtgcgaa gaggagagga tcgctgttcg agtcatcgaa
     4981 ctgccagagg gcgctgcgcc agcccttgcg ctggtcgcgt ttgggcaggg gtgcgggcgc
     5041 gctgaggccg gttgtcccgg cggccggatc cggtgtgatc tcgaaatagc tgcccggcgg
     5101 gagaacgacc aggtcagcga tctcgggcgg acggttctgc tgcagccagg cgaggttcac
     5161 ctccgccaga gcgggctcct ccttccctcc ctgcagggtg cagcgccact gcaggaaacg
     5221 tgcggcagga ctggcgatct gccagcttcc gccgctgctc tgcaggggct gccattcgct
     5281 ccagctcgcc tccggtttgc gagtgttgcc agagcgggtc tgaaaggtca ttctgccctc
     5341 gccgcgccag cgcagggccc cccaccgggt cagggcggcg gcatcgacgg tctcggattc
     5401 aaagctccct tcgcgcaccc tcgggccgac gcggcaggcg agccccatat tggcggtggc
     5461 gaggatcacc tctccggcgg aggtgcgatg gagggtcggg atctgggcgg cgccgatttt
     5521 gaggagcagg gagatttcgc cggcgctgtc gatgctgtaa agacgtccct cgcggccggt
     5581 gcccaccagc agtttgccgt cgggcccggg cagaagggcc tgcacctcgt cgagtccggc
     5641 tggccagatc gggcgcccat agccatcggc ctcgatgcgc agcagctgtg atttggccgc
     5701 cggggcgctc tcgatgccgc cagccagggg cggctgctga agcgccccgc ttttctcttc
     5761 cgccgcctcc tccggggata cgctctgagc cggtgccgcg gcgagaatcc gggtgatggc
     5821 ggcctcgggg ccggcagggg tcatggtcgc agcgtagagg tgtccgcccg gcatcacggc
     5881 gaggctgtgc acctcctcgc ttccgctgtc ataaagtacg aacggcgcct gccctttgcg
     5941 gatgcgatag accagccccg gccggctgct ccccgcaaag agctcctccc cgtcgagcgc
     6001 caggcagcgc aggtgctcag cctcgctgga gaacaccacg ctcatgcggc cgtcgggcga
     6061 gatgcgcagg agacgggcct tgccgccggt ggccgcccag agatcgccgc ggggatcgca
     6121 gagcagatcc cagatgtagg attgccccgg ttcgcaaaag agcgcagcct ttcccccgtc
     6181 gtcgatgcgg tagataaagc cgttggggga ggtggcgaca tagaggctgc ggcggccgtc
     6241 gtgggcgagg gcatagattt cgggttcttc ggcctgaaaa tagagcgtag tgtcgcccgc
     6301 aggggtgatg cggaaaacct tgcccccgtt gccggtgccg aggtagagat tgccggcggc
     6361 atcgctggcg atatcccaga cgtagggctc gccggtatcg acgatctgcg tggtggaggg
     6421 agcgagggta aggcggccat cggcggagag ggaaaggttg tccagccggc cggtgatgaa
     6481 atcggcaaaa ctctgccagg tgcggagggc gggtcccgcc gcgtccgccc gggcggcggg
     6541 cagcgagaaa aggagcatcg ccaaacggca aatatgtcgg agtgttctgg ctctattcat
     6601 ggtcattcca ggaaaagctg ttcggtgcca tcctgtacat catccggcca gtttgtgcga
     6661 ggctgggtga tacggacggt catgcgcttc aggccggtga tttcgcagtc tgccgggatg
     6721 gcccattcgg cgaggacacg atcccgcagg atgcgttcac tgccgcgtcc gttcatcgtc
     6781 cgcaggatcg aggggggcag cccggtgagc tcctccccat cgaccaccat accccgcgcg
     6841 ggctcgcgga tctgcagata aagattgtcg ctggtgcggc gctcctccag gagcgcgcaa
     6901 agctgggaga aggagtcggg ccggtatttg tcgggattgc tctgcatctc ggcctgaatc
     6961 agtccggccg caccgccggc gatgacggtc agggtgctgg attgaaggta gcgcggcaga
     7021 acgacacact gggtgaatct gatctcctgc ccgctggtgc gctgcagggt catggtgaga
     7081 tggagcgaat ccccggggct cacctccagg cggtcctgac ggatcgaacg gatggcggcc
     7141 acccgttctc cgggctcgac ctgagcggtg acctcgacgg cgcgaagcgg cggtggatca
     7201 aaggtgttga ccgtcagggc tccaatgctc atcgtcacca ggtcgaccgc ttcggcgatc
     7261 tcgctgcccg cacccagaaa gccgagccgc tcctggtagg aaatgtaatc cccgacggtg
     7321 atgcggcggc cgtccttgag atcgatgttg cagctcaggc ggacggtcga gggctcagcg
     7381 ccgagtttgc ccgcaaccag ggcctgaaaa agggccgagc gtaaaaaaaa tggggtcaga
     7441 ttgcgcaggg ccggatcgtc ggccatctcg aaatggaagc tccgccgtcc agccgggccg
     7501 gtcagctcca cctgcaccgg aatccaggcg ggcatcaccc ccaccctgcc gtagacgccg
     7561 gagagacggt cctgacgaat ggtgccgatg acctcgaggg agcgggccag cttgcttgat
     7621 cccatcaggc tcggcagggt ggccaggatg cggctccgcg ccatcggcat ctgcaccggg
     7681 ccgagattga agagatagtg gccgaaagcg aggagctggt ccccctcaac agcagtcacg
     7741 gtgccggtca tgtccatgct aaagtcgcca cggatgaaga gctgcgagat agcgctgccg
     7801 ggttcaaggg gggcgcccga ggagccctcc tgcacgctct ctccacccat catcggagcg
     7861 aagccgaggc tgctccacag ttcggcgctg gcggagaggg tctgctctga aaatccggaa
     7921 aagagcaggg gggtctcgat gcggcgcatc gccatcccgg ccggggaggc cggcaggagc
     7981 ggggcggcca tccgctgcca aaagtccccc tccgcaccga gcagaacagc gcgcaggtag
     8041 tgctgcccgc tctggggggc gcagggccgc tcggtgccgc gtctggtctc gtaatcgctg
     8101 gcgcgcatca tcgaggcgat gggcatcacc ccggcgtagg gttccttttg aaactcgaga
     8161 aatcgcatgg ccagggcgcc cgccagccgt ccctgcaggt agatgggact tccgctcatc
     8221 ccggcgacaa ccccggcttg ctcggcccgg ggtccggtca gacggacgat gatcacatcc
     8281 tcaccgggat agaaattggg cgcgacatcg acaaccacaa cgccgaaggt gtcgatctcc
     8341 tccccctgga acacgctcct ccccaccccc gtcatgccgg gtttgatctc cgcgaccggt
     8401 atggtctcca gccgccagcc ctccgccgca aacagcgggg cgcaggggag cagcaggccc
     8461 gggaggagca ggaggaggag atgctgcagt ttaatcgccg agatgatttc tctccaggga
     8521 aagggaagaa cagaccaagg taaacgcctg gaatggatga attacagatc cccgcccaga
     8581 ggccggatga cgacctcctc catcatcgcc gactcgccgg tcagaagaat ggccgtcacc
     8641 gcctgggcga tctcgtcggc gggcatcatt ctggtgttgt cgacgtcggc atcaccccag
     8701 atcagggtat ccgtcgcgcc gggcataagc gaggtgacac ggatgccgtg cttgcgggcc
     8761 tccatgcgga ggacatcggt gaaaccgagc aggccgtatt tactggcaca atagcccgcg
     8821 ttgttgtagt agggcgtccg ccccgccacc gagaccatgt tgatgatatg gccggacttg
     8881 cgttcgatca tgccgggcag gaccgcctgc gaacagagaa aggcgccctt gacgttggtc
     8941 tccatcatca gatcccagtc gctctcgctg gtgtcgacga tcttgccgaa gatggccacc
     9001 ccggcgttat tgaccagcac ctcgatgggg ccgtgcgtcc tctccactgc gacgaccacc
     9061 cgcttgacct ggtccgggtg ccggacatcg cagggcattg ccaccgccgg ccccccctgg
     9121 ttggtgatct cctgggccac cgcttcaatg tcggaaatgg tgcgcgccga tagcaccagg
     9181 tgcgcgccgt gtgcggcaag tgaaagggcg atagcgcggc cgatgccgcg accggaaccg
     9241 gtcacccaga cgatacggcc tttcaaatcc agttgcgaca tactactccc acgatttgat
     9301 cagattgagg aactccatcc gcgtcgcgcg gtcctttttg aactcgccca gcatcgcact
     9361 ggacgtgacg aaactgttct gcttttccac accacgcatc atcatgcaga gatgcttgcc
     9421 ctccataacc actgccacgc cgtagggttt gagcacctcc atcagggtcc cggcgatctg
     9481 attggtcagc cgctcctgaa cctgcagccg ccgtgcgaac atatccacca gcctggggac
     9541 cttgctcaga ccgatgatct tgcccttcgg caggtaggcg acatgcgccc ggccgtaaaa
     9601 gggcagcatg tggtgctcgc acatgctgta aaactcgata ttgcgcacca ccaccatctc
     9661 ttcgcattct tcggtgaaga tggcatcgtt gaggatctct tcgacgctct ggctgtatcc
     9721 gtgcgtcaga tagcgcaagg ccgcatccac gcgtttgggc gtgcgcagca gcccttcccg
     9781 ctgcggatcc tcacccagct ctgccagcat ttccctgacc aacggttcca agctcttggc
     9841 cacaccgggt tcccttcgtt taattttccc ctcctggatc accgcccgcc gcgatacacc
     9901 gccatattac gcggagtttc ccagagtttg agttcataca gctcacagcc tgtgaagcgg
     9961 tccttgagct gctcccagaa ggcgatggcg atgttctctg ctgtgggcac aatgccctgc
    10021 atgaaatcga catcaaaatt gaggtgtttg tggtccaccc ggctgatcac cgcttcctcg
    10081 acgatctcct tgagcacctt gagatcgatg accataccgg tcaccggatc cggctcgccc
    10141 gcaaccgtca cctccaggtc atagttgtgg ccatgcccaa gggggttgtt gcaggcaccg
    10201 aagatggatt ggttctcttc gctggagagg agatcgctgt gcaaacggtg cgcagcattg
    10261 aaatggagca gacgggtgac gtagacaagc ggcatggtgc tctccccgcg ctggaaggac
    10321 tctgcggcgt gaccggcaga tcgcatcagc cctgatcacg tgtaataata caaaaactct
    10381 cagaatgaag caaattgaat tttcaccact gcagcagcga tgacgacacc cggcagggag
    10441 ctgctcagcc gcagaccgcg ccgatgacgc gcataaaatt gtcccaactg atcgcctgga
    10501 tctcatcgag gctgtaatcg cgctcataaa gcccttcctg gatccggcgc atgctggcgc
    10561 acccggcgac cccctcggga aggatcggca cgccgtcaaa atcggatcca aaggcgacat
    10621 attcggcgcc gatcagctcc gtgacgtaga ggatgtgatc gtaatagcgc tccagcggca
    10681 gcagatcgtc gcccatggcc tcggtgaatc gggccacaaa tcccgggtag agctcgccga
    10741 tcttgacgat atccatgccc gcggcccgct ccattttgcc cagctcgctg aagaggtctc
    10801 cggcccgctc ggtgatgcgg cggctgtaac cggcgtcgag gaacccggga tagaggttca
    10861 gccccaccac tccgcccgag taggcgaggc cggcgatgag ctcatcggaa agattgcgcg
    10921 cattggggca aagggttttg gcgcaggagt gtgaggcgat gaccggcttg cggctgtgcc
    10981 ggagcacctc ctccacggtt cggtcatgac tgtgggagac atcgatgatg atgcccaggt
    11041 catccatggc ctgaatcacg tcgcgcccga ggtcagtgag gccgtcgaat cgcggcgcgg
    11101 gatcattgga actgatcgcc cattcattgc tttcggcatg gatgatggtc atgaggcgga
    11161 cgccgagatc acggagcttt tccaggttgg ccagatcgcc gtcgatggca ttgccgttct
    11221 ccaccccgat gaaggcggcg atccggtttg aggcgcgggc gctgccgatc tccgccagat
    11281 tggtgcagag ggcgatctcc ggatggcgtg ccagttccgc tttcaggttg acgatcattt
    11341 tttccaccat cggaaacgcc tggcccctgg ggatggcggg cggaatatag caggcgaaga
    11401 cctgcagacc aatccccccc tcaatcatgc cggggagatt gacctgcatc cctctgtcgg
    11461 tgagggggga tccgaggagc aggttggtgg cggtgtcgca gtgaagatcg caaatcggta
    11521 tcgatccgct ggacggcatc atgtgcaaat ctccttgtta gccatcagca ggccggagtc
    11581 tcctccggcc gcagagtgta cacccgggcc ctgcaaaggc cgggcggccg tgccaaacct
    11641 gccggcccgg catcggccgc ttcccggaca ggttcaggcg cagcgtctat tcgagctggg
    11701 aggtgcagtt ggggcagcgc ctggccttga gtggaatggt cgaaaggcac tgcggacact
    11761 ccttggtggt cacttccgcc ggtgcttcct ctttccgttt cagacggttg aggccgcgaa
    11821 tgaggaaaaa aatcgccagg gcaacgataa gaaaattgat gacagcgttg acaaagactc
    11881 cgtaattgat ggtcaccgca ccggctgact tggcggcggc caggctggcg tacggcccgg
    11941 cagcggcgcc ctctttgagg acgacaaaaa ggttgctgaa atcgacattg ccgagcagca
    12001 taccgatcgg cggcatgatc acatccgcca ccagggagct gacaatggag ccgaaggcag
    12061 cgccgatgat gataccgacg gccatatcga cgacattgcc gcgcatggcg aattctttga
    12121 attccttgaa catatcgcac cctcctgttg ctggttaacg tgccccactg cttcttccgc
    12181 cccaatccgg acgatcaggt ccagatgtga tcgccggcgt gaatgacagg ggctttcact
    12241 ctgcccccga cagccttgcg tgccagtgct gcggcccagg ccgccccgcg ctcgatttcg
    12301 ctctccggca gcagccggcc ccgatgctgc agataaaagc tgaccggctt gagcatgggc
    12361 ttgcccccca gcccctgcag ccgccgccag atcagggtgg ctgcgctctc cccctgccag
    12421 aaatgatgcc tcttgtaacg aatttcaaag acgccgcagt tgagatcctg caccacgcgc
    12481 ggcggcagct tgaagagcca ggagagaatg ccgtcgggct ggtgatgacg gtgatgggga
    12541 cagccaataa tgagcagatc caccccacta ggcggtcccg cctcctccac tggcagcagc
    12601 cgcacccgat gctcgcgggc gagcgctccg gcgatggcct gagccaatgc ggcggtgttg
    12661 cccaacctgg attcgtagac cacccaaacc agcatgctct tttcccaaca aggagtgtcg
    12721 caggggcccc aagcggttct tccctctccg tcgtccgatg ccctgggtgt accgatgggc
    12781 gccgccgcag ccgtgagact gggctgcgcc atctccgctc tatttcacat agtcaatcac
    12841 gccctcacac agccaattgg ccaccgcctg gcggttgtcg gcaatgatga agcgatcctg
    12901 gtcctttttg ttgcgaatat tgccgagttc gatataaacg gcaacgggtt tggcctcgct
    12961 cagtacgtgc agatcccgcg tcgagaccga tccctcatac ccccgtcccg gctgcttggc
    13021 cgcatatttt tcatccacct tggcgagcag ggtattggcc agttttttgc ttttaggatt
    13081 gttggcctga taataataaa agatatcaat gcgcttgcca tgcgaccgcg aatcgagatg
    13141 aatggtgacc atgatctgct gccgggcttt tttgcggtgg cgttcgtaga gattgttgac
    13201 gatatcgacg cggtcgctca accggcgcag atggtccctg gagatgggaa gacggcccag
    13261 atagtattca tgatcatcgt gcttgaggat ggccagatcg cggatgccat cgtcttcatc
    13321 ctggaccacc atgtagacgc tggcgccatg ctccagcagg cggcgcgcca gcctcagggt
    13381 gatgtcgtag gcgtactcat cctcatagag ccggtcgcgc ccgcgctggc cgatcgcgcc
    13441 cggatcagga ccgccgtggc cgggatcgag atagatgaca catcccttga gcgactggtc
    13501 cttctgcttc accgactggt aggccgggcc gaaaatggga aagctcgccg cctcggccgc
    13561 ggcgctgctc tcggagagcc aatacgccgg caccaggagt tcgcggctct ttttgaaggg
    13621 ggccgcgagg acgccggcct cgtacaggga gtggttatag gcctcaatcc tcctggccag
    13681 ggccagatcg ctgatcccga gcgaggaacg gatactggcg ccgtcgtagt agagcttgta
    13741 gaccggcagc ttgtactcaa agcccaggcg gagatattcg ccgccgcgga ccagatcgct
    13801 gttgagctcc cagaaacggg cgatcatcac cttgctgggc gggaggccat agcgcttcaa
    13861 cagggtatat accccatcac tcttgccggg cgagaccgtc acgtactcct gggcatgcag
    13921 ggccgcgccc gcccaaaaca ggaacaatat aatccccgca cggagtccgg cgggcatcct
    13981 cttcctcacc tcagacccgc cacgtcaatc caccttgacc tttttgggat cgaaatcggc
    14041 ctcgggaaag gccatcttca gcccttccag cttgtcgatg atagccgagg ccacgacaag
    14101 attacggtac catttcttgt tcgccggcac tacgtaccag ggcgcccatt cggtgctggt
    14161 ccggctgatc gcctcctcat aagcgcgcac gtactcatcc cagagtttgc gctccttgag
    14221 gtcatccaca ttgaacttcc agcgcttctc cggatcatcc cggcgcgcct caagcctcgc
    14281 tttctgctca tccttgtcga tatggaggaa gaacttgagg atggtcgtcc cctcctcggt
    14341 gagctgtttt tcaaaggcat tgatctggtc gtagcgacgg gaccacacct ccctgggcac
    14401 cagctcgtga acgcggacga ccaggacatc ctcataatgg ctgcggttga agatgacaat
    14461 ctcgccccgg gcgggggcct gctgatgaat gcgccagaga taatcatggg cgagttcgat
    14521 ggcggtcggg gttttgaagc tggcgacccg cacgccctgg ggattgaccc cttcaaagac
    14581 atgacggatc accccgtcct tgccgccggt gtccatggcc tggaggacca ccagaaactt
    14641 ccgcttctgt tcggcgtaga gcagctcctg caggccttcc agcttcttgt tgagcttgag
    14701 cagctcctcc ttgccctcct tcttgtcgcc atcaaagttg gacgagtcgt ttggatcgag
    14761 ttttttcaga tcgaccggct tgcccggaag gatccggtac ttatccatac tcttgtcgtt
    14821 ctttttcatt ctctgtttcc tttccgctgg gataggctgc gggtcccctg gtcagcacca
    14881 ggagctccgg actcaggaga gaccgatcac cttgccgctt tccgtatcga tatcgacatc
    14941 caggaagccg ggccgggtcg gcaggccggg catggtgcgc atctgcccgc agaggggata
    15001 aagaaatccc gcccccacag aggccttcat ctcggttacc ggcagccgcc agccggtcgg
    15061 cacccctttg agggcgggat catggctgag cgagagatgg gtcttggcca tgcatatggg
    15121 aaggctgcca aggccaagcc gggtataata atcaatggcg ctttccacct cagggctgta
    15181 gtcgaccccg tccgcgccgt agatcgaggt ggcgatggca tggatcttgt ccttgatcgg
    15241 ccattccggg ggatagagga agcggaagtt gttcttctgg ccggtcgcgt ccatgaccgc
    15301 ctccgccagc tcgataatgc cggccccacc gagtgcatag tggttgcaga cataggcccc
    15361 gtgagcaccg gccgccaggg ctgcgcgctt gaccacggcg atctcctccg gggtatcgcc
    15421 cggaaaggtg ttgatcgcca ccagggccgg gatgccgaac tggcgggcga tatcaatggc
    15481 cttggcgagg ttgctgcagc ccttctcgac cagctccggg ttggattcgg agtagaccgg
    15541 atcgaggggc ttgcccgcca ccacggtcgg accgccgccg tgcatcttga gagcgcggat
    15601 gctcgccacg atcaccgcgg catccggctt cagtcccgaa taacggcact tgatatcgac
    15661 gaacttttcc aggccgatat cggcgccgaa accggcttcg gtgatgacat aatccgagag
    15721 cttgagggcg atgcggtcgg cgatgatcga ggagttgccg tgggcgatat tggcaaaggg
    15781 gccggcatgc acaaaggccg ggctcccctc gagggtctgc atcagattgg gcatgatcgc
    15841 atccttgagc agcaccgtca tcgccccggc ggcgccgata tcttcggcgg tgacggcctc
    15901 ggagctgcgg ctgcgggcga ccaccatgcg gccgagacgc tcgcgcagat ccgccagccc
    15961 cgtggtcagg gcgagcaccg ccatgacctc gctggccacc gcgatatcat agccggtctc
    16021 gcgctcctgg cccttctcct catcgcccag tccgatgcgg atccggcgca gcagccgatc
    16081 gttgacatcc acgacccgct tccaggtgat gtgctccgga tcgatgtcga ggcggaagag
    16141 acggcgcatc tcctcgaggg agaggtcggc aagacgctgg gccttgatgc cgagccgctc
    16201 catccggcgc cgctgggaga tggcgaactc gcccttcttg cagagcagcc gcgccagctg
    16261 ttcgtcatcg ctgcgcttgc tctcgtgcag cacccgggcg tcgatggcgg ccgcgagcag
    16321 gttggtggca gcggtgatgg cgtgaatatc cccggtcaga tgaaggttaa agtcctccat
    16381 cgggacaatc tggctgtagc cgccgcccgc ggcccctccc ttgatgctga aggtcgggcc
    16441 ctgcgagggc tggcggatgc aggtgaagac gcgctgctta agatgggcgc ccagggcctg
    16501 ggaaagtccc agggtggtgg tggtcttgcc ttcacccagg ggcgtcggtg tcgtcgcggt
    16561 cacgacgata tatttgccgt tgggccggtc cgccagacgg tcgagaatcg agagccggac
    16621 cttggccttg tagtcgccat aaggctccag ctcctcaggc agaatcccgg cttcgcgggc
    16681 caggctgttg atttttttca tgcgcgcggc ctgggcaata gcgatgtcac tcggcaccgg
    16741 ctgcacggga tggagacgga tgggacggta gggggacaca ctgacttgta caggctttgc
    16801 tgttcgggca attttactca tcatggcatc tcctcttttt cacgcctcaa tgtaagcttt
    16861 atttccttgc cagtctacat ttttttaacg ccgcatctca aaaattcagc cttcggggcg
    16921 aaaacccgct cgcaagccag aattttcttg actctaggac taaatatatg taatttttaa
    16981 tataatgact agaatcaaag gctaaggagc ccccatgtcc accctcaccg gctatcagcg
    17041 gcgttatctg cgcgggctgg ccaatcccct gaaaccctgt atccagatcg gcaaaaacgg
    17101 cctgaccggc caggtcatcg aggctatcga tgccgccctg gctgatcatg agctgatcaa
    17161 ggtgcgtttt ctggaactca aagaggagcg caagcagatc agtgccgaga tcgccggccg
    17221 gaccggctgc gagctggcgg ggctcatcgg ccatgtggcg atcttttacc gctgcagcac
    17281 tgatcccgca aaacggaaaa tccagctgcc ggtcaaatcc gcctgacgat gtcgtaccgg
    17341 gcggcgctgc ggccatgcgg ccggagcccc gtgccgcttc accgcgacgc cgggggtgaa
    17401 aactgcccgg ctgcgccaga accacgctag catacaggaa agcaatgaca ctggatatta
    17461 ttgttttatt ggccctcatc ctcgccaacg gcgttcttgc catgtccgag atcgccgtgg
    17521 tctcggtgcg caagacccgc ttgcagcagt tggccaacga gggccacaag cgggcgcagt
    17581 cggtgctcga cctgaccaac aatccctccc ggtttctctc caccgtccag gtcggcatca
    17641 ccctggtcgg catcatggca ggcgccttcg gcggcgcccg cttcaccgag ggactggcgc
    17701 agtggctggg caagttcgaa gccatcgcca gctacgcaca tcccctcgcc ttcgccatcg
    17761 tcgtcctgat catcacctac cttaccctca tcctcggcga gctggtcccc aaacagatcg
    17821 ccctgcacaa tccggaaaag accgccgggg ccctcacccc cacgatgcag cggctcgcct
    17881 ggatcgcccg gccggccgtg gccatcctca gctactccac cgacaccgtc ctgcgccttt
    17941 tcggcgtcag caaggtcgcg gagcagccgg tctctgagga ggagatcaag gtgatgatcg
    18001 accagggcat cgaggccggc atgttcgaag aggccgagcg cgacatgatc gagagtgtct
    18061 tcagtctggc cgacaagcgc gccggcgccc tgatgacccc gcgcacggac gtggtctggc
    18121 tctcgaccca ggatgccccg gtggagacct acaagaaaat cctcgagagc gagcactccc
    18181 gctttcccgt ctgtgagggg agcctcgatg aagtgatcgg ggtggtgcgc gccaaggatc
    18241 tgctcgaaaa cgccatctcg agcagggaaa tcgacctgcg caaacacatg cagccggccc
    18301 tttacgtgcc ggagagcgcc atggccctga acgtccttga gaccttcaag aagcagcggc
    18361 agcatatcgc catcgtcgtc gacgagtacg gcgtcatgca gggcatcgtc accatcttcg
    18421 acattctcga agccattgtc ggcgacatcc cctccatgga tgagaccgac gaccggccgg
    18481 gagtgcagaa acggctcgac ggctcctatc tcatcgacgg cctctattac atggaggagt
    18541 tcaaggagct cttcgagatc gacgaggatc tgccggagga gagcagctac cagaccctgg
    18601 ggggatttgt gctcatgcac ctcggccgca ttccggtagc caccgatttc ttcgagtgga
    18661 gcaaatggcg attcgaggtg atggatatgg atggcaccag ggtggacaag gtgctggtca
    18721 cgccgctcaa cattcaggaa ccggacgcgg ccgtaaatga actgcagcaa atgaacaacc
    18781 ccaaggagcg atgatgcatt accgcacaat tgagctgatc ggccgcagcg tgccgaccct
    18841 ggagggcgcg ggtgtacgcc tgcagcgcgt cttcggattc gggcaggcgc ccaacctgga
    18901 tcccttttta ttgctggatc atttcggctc gaagaatcca gaggactata tggcgggatt
    18961 cccctggcac ccccatcgcg gcatcgagac ggtcacctat cttctcaccg gcgcagtcga
    19021 tcacggcgac agcctcggta ataagggcat catcggcagc ggcgaggtcc agtggatgac
    19081 cgccggcagc ggcatcgtcc accaggagat gccgcgccac gtcgacgaac ccctcaacgg
    19141 cttccagctc tgggtcaacc tgccggcggc gcaaaagatg atgatcccgc gctacaatgg
    19201 cattctcagc tcgcagatcc cggcggtgga gctcgagccg ggcatccagg cgaaggtgat
    19261 cagtggcagc ctggcgggcg cacagggccc gatgaaagat ctggtcgtcg atatcggcta
    19321 ttacgatctc accctggccc ccggatcccg gatcggcctc cccctcccgg ccgggcaccg
    19381 caccttcgcc tacctctacg acggccgcgg ctctttcgcc gctgcggggg accagctcgc
    19441 cggcgccggc cagctcatcg tcttcgccga tgacggcgag gctgtgcaga tccaggccgg
    19501 cgaggagggg atgcgcttcc ttcttgtcca tggccagccc atcggggagc ccgtcgcctg
    19561 gcgcggcccg atcgtcatga acaccgacga ggaactgcgc accgcctttg ccgaattcag
    19621 ggccggcacc ttcatcaaac acaagccggc ctgaccagag cacgaatgaa aaccatctcc
    19681 gccctgcgcc tcatcccgct tgttctgacc ctgtccctcg ctgcggcgct gggccaaaac
    19741 aggagcatcg agctgcacag ggcttccgga cccattatca tcgacggctt catcgagccg
    19801 gcctgggcca acgctgattc agccggcgat ttcttccagc tccagcccta ctacgcccgg
    19861 cctcccctgc accgcacgat tgccaaactc ctcaccgacg atagaaatct ctactgtctc
    19921 atcctctgcc aggatgagcc tggacacatc cagcgcaaca ccggcaaaca tgacgatttc
    19981 agcggcgata tcgtctccct gatgctcgac accttcagcg accgccgcac ggcctacaag
    20041 ttcgcggtca acgcaggcgg cagccgcgcc gactgccgcc tgctcgacga cgcccgaaac
    20101 cgcgattaca gctgggacgg ggtctggttc tccgccagcc gcatccacga ctggggctgg
    20161 gccgtcgaaa tggaaatccc ctaccgctcc atccagtacg gcgaaggact cgacgagtgg
    20221 ggcctcgact ttgaccgctg gagcgccacg gaacaggagg acacctactg gaatccctac
    20281 gatcaggccg aaggtcagcg tatctccaaa ttcggccggc tcctcttcac ggactatcat
    20341 ccagcagtcc acgtcatcaa tctcgaaatc tatccggtcg gcttgaccaa agccgaacag
    20401 gaacgcggcg atggctggag tttctccccc aacgccggcg tcgatctctt ttataaccca
    20461 tcccagcgcc tcaccttcca gctcaccggc aatccggact tcgcccagat cgaggcggat
    20521 ccctttgaat tcaatatttc gcgttatgaa acctactact ccgagcgccg ccccttcttc
    20581 accgagggca atgaggtctt tatgccctcc ggcaagcagc gtgagagcgg cttctactcc
    20641 ccaatggagc tcttttactc ccgccgcatc ggccgcaaac tgcccgacgg cagcgaagtg
    20701 cccctcctcg tcggcagcaa ggctttcggc agggtcaaca gctgggagta tggtggtttc
    20761 atcgccgcca ccggcagaga agagttccag gagggaacag ccagcggcag tgagcccgcc
    20821 gccctctaca gctctgtcag gcttaaaaaa caggtgatgg gcaactcgtc ggttggcatg
    20881 ctctatgtgg ggagaaacag cggagatgag aacaacgggg tggtcgacct ggacggggct
    20941 ttccgcgcct cggactggca gctctcctat caaattgccc gctcctacag gaacgatgag
    21001 ggcgattatg ccgcctcggt cgggctctgg atgcctaaaa cctcctggat tctcgggctg
    21061 cgctcgcgct atgtagggga aaaattcgac atccaggagg tcggcttcgt cccctggcgc
    21121 ggcacctgga acggcgctct tttaaccggg ccggtctggc ggctgcagac cggcgccgtc
    21181 agccagatca tggtctacgg cggtggcctc ttcaaccatg aaaaggtcga tggctatgcc
    21241 gaccgcatgg ccgcactggg ctacaacatg cagttccgca aaaactgggg ttttgagatc
    21301 ggcgtcaccg ccggtcgctc cagggacatg ggggtcctct acaacaaccg cgaggcgacc
    21361 ctctcctcct ggttcaacct ctccccggtc tgggagggca acctctacgg gggctattcc
    21421 cgcacctaca atttctcccg cggctacctc gcctcctatg gctgggcggg cggtgaaatc
    21481 gactggaagg cggccagaat cctagcactg gggagttcgg cggatatttt tttcgaggga
    21541 aatccggcgg gggctgtgga ggagatcacc ttcaacgcgc gcccctatct ctcatggaca
    21601 ccggtcaaca acctgaacat gcggctctac gtcgacaatc tttaccttcg caccaccggc
    21661 cacctcgaac gggtgatcgg cggattcctc ttttcctaca acttccgtcc caaaagctgg
    21721 ctctatttcg ccctcaatga agtgcaggac cgcagcaacg aatatgacca tgaggagcag
    21781 ctcctgccca accgcctgca catggttaac cgggtcaacg tagtcaaact gaagtatctc
    21841 ttctatttct agacagcttc acagcccatc gcaccgaaag agacagcctc cagcggataa
    21901 ggcagccggc ccatatccct gcgcaccggg gagccctttc atgccccggc gggcggcggc
    21961 ggcatgatct gccagatctg ccggcagtag tcgcgaatgg aacggtctga ggagaatttg
    22021 cccatgcgcg cggtattgag gatcgacctg cgcgtccagc cctcgcgatc atggtaagcc
    22081 tccccggccc tggtctggca agcgacatag gcgtcaaaat ccgcacaaag catgtactca
    22141 tccctgttca gcaggcagtc ggtcagggga cggaaaagat cgggctgatc cggagagaaa
    22201 cggccgctgc ggatgagatc gatcacctgc cggagcaggg gattcgagtc gtaaaaggcc
    22261 gccgggttgt agcctgctgc cttcatcccc agcacctccc cggcggtgag gccgaaaaga
    22321 aaaaagttct cctcaccgac ctcctcccgg atctcgacgt tggcaccgtc gagggttccg
    22381 atggtcaggg cgccgttgag cgcgaacttt atgttgccgg ttcccgaggc ctccttgccc
    22441 gcggtcgaaa tctgctccga gaggtcggca gccggatgga tatagtgggc gttcttgacg
    22501 ttaaagtcgg ggaagaagac caccttgagc ctgccgttca catcgggatc ggcattgacc
    22561 acctccgcga cggcggtggt cagcttgatg atcagtttgg ccataaaata gccgggcgcg
    22621 gccttgccgg cgaagatgca ggtgcgcgga actacctcgg caccgggatc ctccttcata
    22681 cgcaggtaga gggcgatgat gtgcaagagg ttgagatgct ggcgcttgta ttcgtggatg
    22741 cgcttggcct ggatatcgaa aagggaatcg ggggagatct cgatgccggt aaccttgcgg
    22801 atgcgttcgg cgagccgttt tttattttcg agcttgaccc gacgccactc ctcgccgaac
    22861 tcagcatcgc cggcaaaggc ttcgagctgg cgcagctgct ccagatccct gatccaaccg
    22921 ctgccgatcc ggctgctgat cagcccgctc agccccggat tacacaacgc catccagcgg
    22981 cggggcgtca ccccgttggt cacattgaaa aaacgttcgg gccagagctc ataaaaatca
    23041 tgcagcacag tgcgcttgag cagatcgctg tgcatccgcg ccaccccgtt caccgcatgg
    23101 ctgccgacac aggcaagatg agccatgcgc agggagcgtt cgcccgcttc gtcgatcagg
    23161 gagagccgtt ccgcccgctt ctcttcgccg ggatggcgtt ccctgacctc gtccagaaag
    23221 cggcggttga tctcgaagat aatctcgaga tggcggggca gcaaatcccc gaaaagtttg
    23281 agcggccagc gctcgagggc ctcgggcaga agggtgtggt tggtgtagca gaaggtgctg
    23341 cgggtgatct gccaggcgcg ttcccactcc atgcgatgca catccaccag caggcgcatc
    23401 agctccgcca ccgcgatgga gggatgggtg tcattcatct ggaccgcaaa atggtcggga
    23461 aagttatcca gattggcgtg ccgtgagaga tggatccgga tcatgtcctg caatgaacag
    23521 ctcacaaaaa agtactgctg aatcagcctg agctgcttgc ccgtttcggt ctcatcgttc
    23581 ggatacaaca ccttggtgat ggtctccgag gagattttgt cctccacggc agcgtaatag
    23641 tcgccctggt tgaaatccgc aaaatcgaaa gagacgatgg cctccgcctt ccacaggcgc
    23701 atggtgttgc acttttgatt ggcgtagccg atgatggggg tatcgaaagg gaggcccttg
    23761 acatacctgg ccggtatcca tttgacccga taccggccgt tttcgtcggt ccaggactct
    23821 gtgcggccgc ccaggccgac cttctggcac catttggccc gtgcgatctc ccagggattt
    23881 ccgaaccgaa gccaggtgtc agcgatctcg cgctgccagc cctccctgat ctcctgatcg
    23941 aaaatgccga attcatagcg gatgcc
//
LOCUS       DAOPWU010000014        23277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_2189_length_23277_cov_5.187408, whole genome shotgun sequence.
ACCESSION   DAOPWU010000014 DAOPWU010000000
VERSION     DAOPWU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23277
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2189_length_23277_cov_5.187408"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PKM23_01805"
     CDS             <1..1235
                     /locus_tag="PKM23_01805"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNY90236.1"
                     /translation="TCTELPERVFLEVRALYPSAWFPGMAVTAGTVRPGILAEEARWA
                     AEANALRQKEEAKQAVRAVRRVEGRYYAGVLALIGLGIWYYFFRSYRKRPVVHDFIPK
                     ESPEIPDETPPALVGYLLSSRMITGGALLATLYDLARRGFVRISEKVSEGRGLFGGKK
                     SDYMLTLQSDEARARRGELQEYESGLLDFIFNDLSGGESEVALKAFSKQASKMQKFFG
                     EWQKIVKKTAEAAGWYDQRSRSGMYRAMACCGVGMVVSLLLSIWYAPWTLAAAALFLA
                     LLIGAAGILHRTEEGERKFKRWKALSRYMRGKGMMQSPQGSLLDRIDRYFVYAPVLGA
                     GKPQYQALANQIPEGQHAVYMPWFLYTSSGGAAGHAESFAQAFTAMTTTMSSSGGVGG
                     GASVGGGGGAGGGGGGAG"
     gene            1361..1849
                     /locus_tag="PKM23_01810"
     CDS             1361..1849
                     /locus_tag="PKM23_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNY90237.1"
                     /translation="MPIQDNQVVTIQYNLTDADGNLLDTTYEEGPMEFITGSGELLPK
                     LEAEIITMPLNGKKKIVLQPAEAYGDYDPEDIQVVKRDELPDDIELEIGTELLAEVEE
                     DAEEELSCFISKIDGDEITLDFNHPLAGKSLHFEVELTGMRPATQEELEHGHVHGDEE
                     FE"
     gene            complement(1925..4777)
                     /locus_tag="PKM23_01815"
     CDS             complement(1925..4777)
                     /locus_tag="PKM23_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNY90238.1"
                     /translation="MSTAFHAKFYAHALTQHYRGDLLGKISHSLFDAHVDLNPHQLEA
                     ALFALRSPLSRGVILADEVGLGKTIEAGLVLCQYWAERRRRLLIICPAALRVQWGLEL
                     TEKFHLPNRIIDARTMRQVQREGKANPFDQPLVLITSIHFASRHGALLRTIPWDLVVI
                     DEAHKLRNVYQTGSKMAQEIQWALSERQKLLLTATPLQNSLLELYGLATLIDSHIFGE
                     VEAFRSRFGGPKPDHAELRERLTSFCKRTLRSQVAEYIQYTERRAITLPFTPNEAEQS
                     LYDALSDYLAREETLAVPRQQRHLLILILRKLLASSAYAVAGTLTAMRSRLELMQRGQ
                     QPDAGFIDGLIQGEELESDYIEESDAGGEGGADGEADPVRLAEEIRLLEGFIAAAGAI
                     ALESKAEKLGQALECGFAEMKKMGAPEKALIFTESRRTQEYIYRHLLRIGYRGRIVLF
                     NGSNNDAESAAIYQEWLARRSSEQRSGGSRQIDIRTALVDHFRDQAQIMIATEAAAEG
                     LNLQFCSLVINYDLPWNPQRIEQRIGRCHRYGQKHDVVVINFINTRNQADQRVYELLE
                     QKFNLFNGVFGASDEILGQIESGVDFEARILDLYQRCRTRDEIDAAFSALQQEMEQSI
                     THQLDKTRRLLLEHFDQDVQARLRTRLEDARQQLNRIGHFFWELTRYQLADQARFNDA
                     GLQFDLVQSPLAQVPSGRYALVSRSRAPVDDEALYRLSHPLGEFVLAAGKRTATPVAH
                     LFFDISHHPTRISMVEALRGRSGWLSLGKLEIDSLEREEHLIFAAVDDQGRILDGELC
                     EHLFYCRGAVMPVQPPAEAVLKKLARESEKQIKSLSHHSQEWNNTLFNEECERLETWA
                     DEAAEAVESELDEIKKEIRRLSRLSRRTRIPLEQHQLQRQLQELEQKKRRLRQRIFQV
                     EDETGIKRDTLIAMLEKRVAQKSEVSELFTIRWSVV"
     gene            complement(4846..6324)
                     /locus_tag="PKM23_01820"
     CDS             complement(4846..6324)
                     /locus_tag="PKM23_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNY90239.1"
                     /translation="MPGTLESRFAPFRDHIIGYNQSFATAYGEHRIVYADWTASGRLY
                     RPIEEAMSGRFGPFVGNTHSESSITGTSMTRAYHLAQERIKRHVHAGPDDMLIATGAG
                     MTAAVNKWQRILGLRLPEQLADYLYLPQKSRPVVFITHMEHHSNQTSWLESIADVEVI
                     EPDAQGRVNLDDLERLLRIHRDRQMKIGTFTACSNVTGICTPYHEMARIMHEHGGLCF
                     VDFAASAPYVAIDMHPADPLARLDAIFFSPHKFLGGPGSAGILLFDASLYTLRVPDQP
                     GGGTVNWTNPWGEHSFVDNIEAREDGGTPGFLQAIRAALAIELKERMGVADMLAREEE
                     IKARLFAGLRAIPDLHLLADHIEERLGIFSFYSEKIHYNLFVKLLNDRFGIQVRGGCS
                     CAGTYGHYLLHVDPHRSHRIAEQIDHGDLSSKPGWVRLSIHPTMTDAEIDFILAAIRE
                     TTARAGEWEKDYSYSPSTNEFRCLRASGVLAPDVASWFKLGE"
     gene            6548..7180
                     /gene="lexA"
                     /locus_tag="PKM23_01825"
     CDS             6548..7180
                     /gene="lexA"
                     /locus_tag="PKM23_01825"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNY90240.1"
                     /translation="MSDTLTPKQRQVLEAIRRFQQEKGYPPTVRELAELFGQLSTAGM
                     HKILMTLQEKGVLSRREGTKSRSYHIIGEEPGEGPAGRARPCPIIGEVVAGMPELALE
                     EREGDLYLDSDWVGKEDIFLLRVRGHSMIDADIRDGDLLAVQITQSCRNGEIIIALLD
                     DEATVKRFYKEKDRVRLQPENQTMQPIYIPIDDPGFRIIGKVKGLLRRYH"
     gene            7195..7611
                     /locus_tag="PKM23_01830"
     CDS             7195..7611
                     /locus_tag="PKM23_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90241.1"
                     /translation="MATLAETIQIEQPRPRPRTELQEPALLMRTASRRVSSWFTTLEE
                     RHNITAGEVMDYLVQREPRIKEQLDRLWALVDCDVQGRYLEGVLDENDFRLWRQHLRE
                     WLNLLHSALALVRLHRSSDAEGDFALAAGALTPAAA"
     gene            7665..7907
                     /locus_tag="PKM23_01835"
     CDS             7665..7907
                     /locus_tag="PKM23_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90242.1"
                     /translation="MAKSIRTDWAAYWDKFDDLDELQSKSFNLDDTLIEIERYAGCKL
                     LSGDQMQIIMALEERIREVSKRSGQGAVSAQYSLFD"
     gene            8490..11225
                     /locus_tag="PKM23_01840"
     CDS             8490..11225
                     /locus_tag="PKM23_01840"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNY90243.1"
                     /translation="MSSRELEKIYDPTLVEDRWYAFWEENHLFHADARSDRESYTIMI
                     PPPNVTGMLTMGHILNNTIQDLLIRWKKMQGYETLWMPGTDHAGIATQNKVEAALRQE
                     GETRYTLGREKFLERVWEWREKYGGIIFQQLRKLGAACDWERECFTMDEGLSQAVREV
                     FVSLYEKGLIYKGNRLINWCPSCRTALADEEVNSIEEEGRLWNIAYPLAEGEGEIIVA
                     TTRPETMLGDTAVAVNPADERYQHLIGKKVILPLANRAIPVVADEHADPAFGSGAVKI
                     TPAHDFNDFEVGQRHGLELVQVIGPDGRMNAAAGLYEGLDRFEARKKILADLQAGGLL
                     RGEIRKNIPVPHCYRSGDVIEPHLSEQWYVRMAPLAEPALEAVRSGRIKFYPRHWEET
                     YFHWMTNIRDWCISRQIWWGHRVPVWYCDQGHTIVRREAPASCPECGSTALRQDEDVL
                     DTWFSSWLWPFSTLGWPEKSEELKKFFPSDTLVTGPDIIFFWVARMIMASLEFMGDIP
                     FRQVYFNGMIRDLEGRKMSKSLGNSPDPFWLIRGAAAADIGEFSKLNASYRSGVPAYG
                     ADAIRLTMVYLTPLGGDVRFDHTLVEMGQKFANKLWNAARFILMNLDDEETLLPVAEI
                     PAVEMELADRWILSRLHRALAEVERALQEFRLNDATRAVYDFIWGEYCDWYLELIKKR
                     LYDEADPAARRVARSLATHVLESSVRMLHPFMPFITEEIWQHLPAEYQEEKPVRTIML
                     QSYPKADAALLDETAEQEMSVMQQVIGAVRNIRGEMNVPPAREADLIINSAAAAPLEL
                     IERNGGYLHKLARVGRILYNQPRPAAAASAVAQGIEILVPLAGLIDVDRERGRLEKEI
                     ARLEGQLFGLTQKLMNRDFIARAPKEVIEKEEKKRSDYSLTLEKLRASLQSIAGE"
     gene            11300..12649
                     /locus_tag="PKM23_01845"
     CDS             11300..12649
                     /locus_tag="PKM23_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNY90244.1"
                     /translation="MEHPFWLVLFVTLLPLLALAAEPKIAVTVYNEDLALVREARSLK
                     LVKGSQSYAYSGVAAQIDATTVHFRAVDPSAGITLLEQDYEFDLVGTERLLQKYIDEQ
                     VIATAENGAVHSGKLLSAQDGDIVLLDSGGKVQALKAAKLQSVEFPSLPEGLRTRPTL
                     VWLLESSKAGDHETEISYLTGGLSWHAEYVAVLEAAENAAQLNGWVSIDNQSGATYTD
                     ARVKLVAGDVNRIQPEPQVRAMFRKEAAMAMDSQFEEKALFEYHLYTLQRPATLKDKQ
                     IKQLSLFPAAHTPVTKLYSYDGAFDGSKVKVKLEFKNSQANGLGMPLPKGVVRVYKQD
                     TDGAQEFIGEDRLDHTPKDEKVRLSLGNAFDIVGERIVKDVREVSRSSRQETVQIKLR
                     NHKSEEVVVNVIEHFGGDWSFIGKTPTIAVKEAGKAEFQVRVPAGEETAFEYTVLYK"
     gene            12682..14784
                     /gene="recG"
                     /locus_tag="PKM23_01850"
     CDS             12682..14784
                     /gene="recG"
                     /locus_tag="PKM23_01850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNY90245.1"
                     /translation="MNTSPKTALEWPVDRIPGIGQKRATALQNAGLYTIGDLVYYFPR
                     RYLDRSTVTRINRLKPDQEVTVVARVIQFTVQKGRRSRFVLVVGDGTGFLHCIWFSRL
                     PYWYKAFKPGEWLALSGKVGFFKGLQMVHPEFDRLGVTGEGEQVHTGKIIPLYPSSEP
                     LGQAGFDSRGFRRILHTVLKNWGDQIDETIPAELAERLGLAPLRQALSAIHFPADFAT
                     LQAARMRLKFDELFFMELLVALRRRHQQEGTGGIRFEKVGELTRRLVESLPFALTGAQ
                     KRVLRQIRADMKSPRPMHRLLQGDVGSGKTVVALITMLIAVENGYQAALMAPTEILAE
                     QHYLTIREMLRELEVEVVLLVGGQGRSERGELLDRIASGEARIIIGTHALIQESVDFH
                     QLGLVVIDEQHRFGVMQRATLREKGLNPDLLVMTATPIPRTLTMTVYGDLDVSIIDEL
                     PANRKPVKTFWRDESRRSKIYHFLRSRVSEGAQAFIVFPLVEESEKSDLKAAVESYEA
                     MSATFFKDFRIGLIHGRMKPEEKEATMAAFKRHELQILVSTTVIEVGVDVPNASIMVV
                     EHAERFGLTQLHQLRGRVGRGSRQSFCILVSYGALTEEARTRLETLAVTSDGFKIAEV
                     DLQLRGPGEFFGTRQHGLPELRLADPSRDLELLRLARDEAFRLVEADPQLRDQESLGT
                     RHYFVRNYQERFGLAWVG"
     gene            14835..15194
                     /locus_tag="PKM23_01855"
     CDS             14835..15194
                     /locus_tag="PKM23_01855"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNY90246.1"
                     /translation="MEEQNKHHAALLFNLVMMFHSAAMQQMGKLKNPMTNAIERDMEQ
                     ARLSIDMLDMLQAKTAGNLSEDESKFIGHVISELKLNFVDEVNKDKQKAAESATAGET
                     PAEGGTGESAPENRTAE"
     gene            15214..16017
                     /locus_tag="PKM23_01860"
     CDS             15214..16017
                     /locus_tag="PKM23_01860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY90247.1"
                     /translation="MSQKTTLHRREVWDQFWQEREEIEQVYSNSERVIDQLIRLGDPA
                     GKWILEVGAGSGRDSLALSSRGGRVIMLDYSAPALEVIQKLAQKTGRPVYLVRGDAFH
                     LPFRSDALDLVFHQGLLEHFTAPEGILAENLRVLRPGGAALADVPQRYHLYTAVKHLL
                     IAFNRWFAGWETEFSHARLERLFAQTGFVRTEFYGEWMRPSFIYRASREALKKAGITL
                     PLYPPSLPLFGRIRAGLRALLRRHPWHLNTVMDIGAIGFKPVGSDQRAG"
     gene            16023..17162
                     /locus_tag="PKM23_01865"
     CDS             16023..17162
                     /locus_tag="PKM23_01865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY90248.1"
                     /translation="MRILVINWQDIRNPLSGGAEVHFHEIFRRVAAMGHAVTLLCSAF
                     AGAAAEEEIDGIRIVRCGSRQLFNFAVPAAYRRLRKAQAFDVVIDDLNKIPFYTPCFV
                     QEPLLTIVHHLFGRSIYLETSWLPASYVYWSERLALRIYRRTPFAVVSESTRDELRGL
                     GITAPVELLPNAVDHERYPYAPVERSPEPLICHLGRLKRYKSVEHLLRALPRVLSSLP
                     DTRLVIVGEGDHRPALEALAGELGIGGAVTFTGQVSHEEKVHWLNRAWVAVNPSPKEG
                     WGLTVIEANACGVPVVAADSPGLRDSVRDRETGLLYPYGEIEALAGGLITLLCDRTLR
                     ERMGEAARLWAGTFSWDQSARKAVAILEGMGLRSQPGGPAAVGKN"
     gene            17213..19864
                     /locus_tag="PKM23_01870"
     CDS             17213..19864
                     /locus_tag="PKM23_01870"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNY90249.1"
                     /translation="MQKFKLYNRIAAAAAFLLSLVVYLRTIAPTVSFWDCGEFIACSH
                     LLAVPHPPGAPFYLLLGRLASMIPWTGEIALRVNLMSALVTSLTIMLTYLIIVLLIKQ
                     WRGEPRDGADAFILIASGLLGALALAVSDTVWFNAVEAEVYAFSLALTAAVFYLALVW
                     LEKSDQVGAERYLLIIAYLIGLSLGVHLLAVLAVPAIALVIYFKWLKIRDKKFTFGSL
                     LAFMGIALLITVLIYPGTVKLIPALAGGLGLWAVLLLYVLVLAGAWWAVSNRNRMASL
                     VFMSAFLILLGYSTYTMILIRSGLDPVIDENDPETAEGMVKYLNREQYGDWGTFPRRF
                     PGLPQEWQFDQEHPNENYKTWQWSKQMDFLWDYQIKKMYLRYFGWQFIGKGDTRGADG
                     FIAENFTWRGLGMLPFLIGLIGMVYHFRKDWRHSSSILLLFLATGVAIVLYLNQENPQ
                     PRERDYVFVGSFFAFALWIGIGASALFEWIKERYYLIAGNQERGAQKNMLYKGLTGLT
                     LLLLILALPVNLFSFNFRDHDRSGNYVAYDYSYNILQSCEPNSILFTNGDNDTFPLWF
                     LQYVYNIRRDVRVVNLSLLNTDWYIKQLKNEEPRVPISLTDNQIGQLSLIPWEAQKIK
                     IPVPANARSEVLGEIDVASTAHAEPLNELEVNVQPTIMGRALRVQDFMILNIIYANQW
                     KKPINFAVTVSDDNKVNLDPWLRMDGLAFKLVPMDGEVLARERLRKNLFEDFKYRGLD
                     DKSVYYDDNVIGLLQNYRAGFLRLAQEYLMQRNHDKVIETLDRMEKVIPENVIPVPDY
                     RLSLRIGQLYDLAGKSEEFIRRAEEAIKAKPDDPMVYGYLVATLSRSGNHKRAVELLE
                     EWLQRHPEDREAASRLTEERALLSSGR"
     gene            19974..20960
                     /locus_tag="PKM23_01875"
     CDS             19974..20960
                     /locus_tag="PKM23_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNY90250.1"
                     /translation="MSRKKHHSSRQLWRPPAVTLLKIAVSATLIILLLRGIGWAPIRD
                     QFSHALWGSAAASLGLFVVSNFLGAWQWHLLLESKKIHLSFRRVLGYYNIGLFFNNFL
                     IGNVGGDAFRVYDIRRISGETNGALSTVFFDRFVGFFTMASLALFATLMAARQMVSAS
                     ALEAILFIFGAWALASAFLLYEPFAKKFSWIFRLLLPASLHVKAKAFYYSLHEFRSQK
                     RLLLHLLCISLLVQTLRIMTHFFAARSLGVRERASFFFLFIPVVALVASMPISLGGLG
                     VREQSAVTLFSPLGIPAAKIVAFEFLAYLVGVVSSLPGGILFALRRESRTGA"
     gene            21020..21478
                     /gene="purE"
                     /locus_tag="PKM23_01880"
     CDS             21020..21478
                     /gene="purE"
                     /locus_tag="PKM23_01880"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNY90251.1"
                     /translation="MYKAAIMMGSESDREVMSEARAYLDYFGIDYEIKILSAHRMPDA
                     TAEFAKSAEENGFCAIIAGAGMAAHLAGVVASHTLLPVIGVPLSGSALTGVDALYSMV
                     QMPRGVPVATMAIGRAGAANAAIFVAEMLAGRDAGIRDKLVEFRKKGCVL"
     gene            21486..22379
                     /gene="rfbD"
                     /locus_tag="PKM23_01885"
     CDS             21486..22379
                     /gene="rfbD"
                     /locus_tag="PKM23_01885"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNY90252.1"
                     /translation="MRILITGSNGLLGQNLSRLAPAGAELMGIDLQEMVEAPGLSRFR
                     RLDLGERKLLLACVEEFAPDWILNAAAFTNVNGAESARELCWRANVTAVENLALACRK
                     LGSRLVHLSTDYIFDGDHGPYREEDLPNPIGYYGKSKLASENVLRSSEIPYTVVRTMV
                     LYGHARQVKPDFVSWLISSLRQGKPVRIVTDQFGNTTLAEELALGIWRIVERRATGFF
                     HIAGTEIIDRYAFALKAAEVFNLEAGLITPITTAELHQEAPRPMRSGLVVEKALQELE
                     LELSDAAGGLRKLKQQLDAPT"
     gene            22396..23112
                     /locus_tag="PKM23_01890"
     CDS             22396..23112
                     /locus_tag="PKM23_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNY90253.1"
                     /translation="MKTLVIFPTYNEKQNILAIIEAVFAQNIEGLEILVVDDNSPDGT
                     GDLVAGRTGMEPRLHLLRRDRKMGLGTAYVAGFRYALERDYDRIIEMDADFSHDPRDV
                     VRLLEASADSDIVIGSRYIKGVNVVNWPLKRLLLSMFASHYTRIITGLPLRDCTAGFK
                     CFRREVLEAIELDSIQSDGYSFQIEMNFRAWVKGFRILEIPIIFVDRAAGTSKMSKRI
                     LREAVWMVWKLKGLKILNRL"
BASE COUNT         4387 a         7146 c         7358 g         4386 t
ORIGIN      
        1 caacctgcac ggagctgccg gagcgggttt ttctggaggt gcgcgccctc tatccctccg
       61 catggtttcc ggggatggcg gtcacagccg gtacggtgcg tcccggcatc ctcgccgagg
      121 aggcgcgctg ggccgctgaa gccaacgcct tgcgccagaa agaggaggcg aagcaggccg
      181 tgcgcgcggt gcggcgtgtg gaggggcgct attatgccgg cgtgctggcc ctcatcggcc
      241 ttggcatctg gtactatttc ttccgcagct accgcaagcg gccggtggtg catgatttca
      301 tccccaaaga gagccccgag atcccggacg agaccccgcc ggcgctggtc ggctatttgc
      361 tcagcagccg gatgatcacc ggcggggccc tgctcgccac cctctacgac ctggcgcggc
      421 ggggttttgt ccgcatcagc gaaaaggtga gcgaaggccg cgggctcttc ggcggcaaga
      481 agagcgatta tatgctcacc ctgcagagtg atgaggccag ggcgcgccgc ggcgagctgc
      541 aggagtatga atccgggctg ctcgatttca tcttcaatga tctctccggg ggggagagtg
      601 aggttgcgct caaggctttc agcaagcagg ccagcaagat gcagaaattt ttcggggagt
      661 ggcagaagat cgtgaaaaaa accgccgagg cggccggctg gtacgaccag cgcagccgga
      721 gcggcatgta tcgcgccatg gcctgctgcg gcgtcggcat ggtggtgagt ttactgctct
      781 cgatctggta cgccccctgg acgctggcgg ccgcggccct ctttctcgcc ctgctgatcg
      841 gggctgcggg catccttcat cgcaccgagg agggggagcg caagttcaag cgctggaagg
      901 ccctctcccg ctacatgcgc ggcaagggca tgatgcagag tccgcagggg agcctgctcg
      961 accgcatcga ccgctatttc gtctatgctc cggtgctcgg ggcgggcaag ccgcagtacc
     1021 aggccctcgc caaccagatt cccgaggggc agcatgcagt ctatatgccc tggttcctct
     1081 atacctcgag cggcggggcg gccggccatg ccgagtcctt cgcccaggcc ttcaccgcca
     1141 tgaccacgac gatgagctcc tccggcgggg tcgggggcgg ggcgagcgtg ggaggcggtg
     1201 ggggggctgg aggcgggggc gggggagcgg gttgagacgg gcacccgagg gggaggctgc
     1261 agaatcgagc cgttttttgc cttggttttt tgcgatttaa tgcgtattat ctacagaaat
     1321 aaacaagcgg atttcgatca ctgattttaa ggaggtcagc gtgcccatac aagataacca
     1381 ggtcgtcacc atccagtata atctcaccga tgcggacggc aacctgctcg ataccaccta
     1441 tgaagagggg ccgatggaat tcatcaccgg ttccggcgag ctcctgccca aactggaagc
     1501 agagatcatc accatgcccc tgaacggcaa gaaaaagatc gtcctccagc cggccgaggc
     1561 ctatggcgat tacgatcccg aggatatcca ggtggtcaag cgggacgagc tgcccgatga
     1621 tatcgagctg gagatcggca ccgagcttct cgccgaggtc gaggaagatg cggaggaaga
     1681 actctcctgc ttcatcagca agattgacgg cgacgagatc accctcgatt tcaaccaccc
     1741 cctggcgggc aagagcctcc atttcgaggt ggagctgacc ggcatgcgtc cggccaccca
     1801 ggaggagctc gaacacggcc acgtccacgg tgacgaagag ttcgaataaa ccgatgcgct
     1861 gtgcgcgtca ggccggaggt tatccccctc cggccttttt tttatgcccc ggcgcgaatg
     1921 gcgttcatac tacgctccag cgaatggtga aaagttcgct cacctcgctc ttctgggcca
     1981 cccgcttctc gagcatggcg atgagggtgt cgcgcttgat gccggtctca tcctcgacct
     2041 ggaaaatcct ctggcgcagg cgccgcttct tctgttccag ctcctggagc tggcgctgga
     2101 gctgatgctg ctcgaggggg atgcgggtgc ggcgggagag ccggctgagc cggcggattt
     2161 cctttttaat ctcgtccagc tcgctttcga ccgcctcggc cgcctcgtcg gcccaggttt
     2221 cgaggcgttc acactcctca ttgaagaggg tgttgttcca ctcctgggag tgatggctga
     2281 ggcttttgat ctgtttttca ctttccctgg ccagtttttt gagcaccgcc tccgcagggg
     2341 gctgaaccgg catgaccgca ccccggcagt agaagagatg ctcgcagagt tccccgtcca
     2401 ggatgcgccc ctggtcgtca acggcggcga agatgagatg ctcctcgcgt tcaaggctgt
     2461 cgatctccag ttttcccagg gagagccagc cgctgcgtcc cctcagggct tcaaccatcg
     2521 aaatccgcgt cggatggtgg ctgatgtcga aaaagaggtg agcgaccggt gtggccgtgc
     2581 gtttgcccgc cgcgaggacg aattcgccga ggggatggga gagacggtag agggcttcat
     2641 cgtcgaccgg ggcccggctg cgggagacca gggcgtagcg gccggagggg acctgggcga
     2701 ggggtgactg aaccagatcg aactggaggc cggcgtcgtt gaagcgggcc tggtcggcga
     2761 gctgatagcg ggtcagctcc cagaagaaat ggccgatgcg gttgagctgc tggcgggcgt
     2821 cctccagccg ggtgcgcagc cgcgcctgga catcctggtc gaaatgttcg agcaagaggc
     2881 ggcgggtctt gtcgagctga tgggtgatgc tctgctccat ctcctgctgg agggcggaaa
     2941 aggcggcgtc gatttcatcg cgggtgcggc agcgctggta gagatcgagg atgcgggcct
     3001 cgaaatcgac gcccgattcg atctggccga ggatctcgtc ggaggccccg aagaccccgt
     3061 tgaagaggtt gaatttctgc tcgagcagtt catagacgcg ctggtcggcc tgattccgcg
     3121 tattgataaa gttgatcacc accacatcgt gcttctggcc gtagcggtgg cagcggccga
     3181 tgcgttgttc gatgcgctgg ggattccagg gcaggtcgta gttgatgacc aggctgcaga
     3241 actgcaggtt gagcccctct gcggccgcct cggtggcgat cataatctgg gcctggtcgc
     3301 ggaagtggtc gaccaaggcg gtgcggatgt cgatctggcg ggagccgccg ctgcgctgct
     3361 cgctggagcg gcgggccagc cattcctggt agatggcagc cgattccgca tcgttgttgc
     3421 tgccgttgaa gaggacgata cggcccctgt agccgatgcg gaggagatgc cggtatatgt
     3481 actcctgcgt gcgccgcgac tcggtgaaga tgagggcttt ttccggggcg cccatctttt
     3541 tcatctccgc gaaaccgcat tccagggcct ggccgagctt ttcggccttg ctctccaggg
     3601 cgatggcccc ggcggcggcg ataaaaccct ccagcagacg gatctcctca gccagccgga
     3661 cgggatcggc ctccccgtcg gccccaccct caccccctgc atcactctcc tcgatgtaat
     3721 cgctctccag ctcctccccc tggatgagtc cgtcgataaa gccggcgtca ggctgctggc
     3781 cgcgctgcat cagttcgagg cggctgcgca tggccgtgag ggtgccggcg accgcatagg
     3841 ccgaggaggc gagcagcttg cgcaggatga ggatgagaag atggcgctgc tggcgcggca
     3901 cggccagggt ctcctcgcgc gcaagatagt cggagagggc gtcgtaaaga gattgctccg
     3961 cctcattggg agtgaagggg agggtgatgg cgcgccgctc ggtgtactgg atgtattctg
     4021 cgacctggct gcgcagggtg cgcttgcaga aggaggtgag acgctcgcgc agctcggcgt
     4081 ggtcgggttt gggcccgccg aagcggctgc gaaaggcctc gacttcgccg aagatatggc
     4141 tgtcgataag ggtggccagc ccgtagagtt cgagcaggga attctgcagg ggagtggcgg
     4201 tgaggagcag cttctgccgc tcgctgaggg cccactggat ctcctgggcc attttgctcc
     4261 ccgtctggta gacgttgcgc agtttgtggg cctcgtcgat gacgaccagg tcccagggga
     4321 tggtccgcag cagggcgccg tgacggctgg cgaaatggat ggaggtgatc aacaccaggg
     4381 gttggtcgaa gggattggcc ttgccctcgc gctgaacctg acgcatggtg cgggcgtcga
     4441 tgatgcggtt ggggaggtga aatttctccg tcagctccag gccccactgc accctgaggg
     4501 cagccggaca gatgatcaga agcctgcggc ggcgttcggc ccagtactgg cagaggacca
     4561 gtcccgcctc gatggtcttg cccagcccga cctcatcggc gaggatcacc cccctggaga
     4621 gtggggagcg cagggcgaaa agcgcagcct cgagctgatg gggattgagg tcgacatggg
     4681 cgtcgaagag ggagtgcgag atcttgccca ggagatcgcc gcggtagtgc tgcgtcaggg
     4741 cgtgggcata gaatttggca tgaaatgcgg tggacatggc gctttattcc cggatattgg
     4801 cggatgtcga tggatagagg ggcgagagcg gcggatcgag gcggctcact ctcccagttt
     4861 gaaccaggag gcgacatcgg gggcgaggac cccggatgcg cgcaggcagc ggaattcgtt
     4921 ggtggatggg gagtaggaat aatccttctc ccactcgccg gcccgggcgg tggtctcgcg
     4981 gatggcggcg aggataaaat cgatctcggc gtcggtcatg gtgggatgga tggagaggcg
     5041 gacccatccc ggcttgctgc tgaggtctcc gtggtcgatc tgttcggcga tgcggtggga
     5101 gcggtgggga tcgacatgga gcagataatg gccgtaggtg ccggcgcagg aacagccccc
     5161 gcgcacctgg atgccgaaac ggtcattgag cagcttgacg aagaggttgt agtggatctt
     5221 ttccgagtaa aaagaaaaga tgccgaggcg ttcctcgatg tggtcggcga ggagatggag
     5281 gtcggggatg gcgcgcagcc cggcgaagag gcgggctttg atctcttctt cacgggcgag
     5341 catatcggcc acccccatcc gctccttgag ttcgatggcg agggcggcgc ggatggcctg
     5401 gaggaagccg ggcgtgcccc cgtcctcgcg cgcctcgata ttgtccacga agctgtgttc
     5461 tccccaggga ttggtccagt tgacggttcc gccgccgggc tggtccggca ccctcagggt
     5521 gtagagcgag gcgtcgaaaa gaaggatccc cgcactgccc ggtccgccga ggaacttgtg
     5581 gggactgaaa aagatggcat ccagcctggc cagggggtcg gccggatgca tgtcgatggc
     5641 gacatagggg gcggaggcgg cgaaatcgac aaagcacagg ccgccgtgct cgtgcatgat
     5701 gcgggccatc tcgtgatagg gggtgcagat gccggtgacg ttggagcagg cggtgaaggt
     5761 gccgatcttc atctgccggt cgcggtggat gcgcagcagg cgttcgagat cgtcgagatt
     5821 gaccctgccc tgtgcatccg gttcgatcac ctcgacatcg gcgatgcttt ccagccagga
     5881 ggtctggttg gagtggtgct ccatgtgggt gataaagacc accgggcggg acttttgtgg
     5941 aagatagaga tagtctgcca gctgctcagg gaggcgcagg ccgaggatgc gctgccactt
     6001 gttcacagcg gcggtcatgc cggcaccggt ggcgatgagc atatcatcgg ggccggcatg
     6061 gacatgccgc ttgatgcgct cctgggcaag atgataagcc cgggtcatgc tggtgccggt
     6121 gatactcgat tccgaatggg tgttgccgac aaaggggccg aaacggccgg acatggcctc
     6181 ctcgatcggc cggtagaggc gcccgctcgc ggtccagtcc gcatagacga tgcgatgctc
     6241 gccgtaggcg gtcgcaaaac tctgattgta tccgatgatg tggtcgcgga agggggcgaa
     6301 gcggctttcg agcgtgcccg gcatggaact cctgctgaaa tgggttctct ctctatacac
     6361 ccatattgta acaaaaatct ccgacaggaa caacgatttt cttgccccca taaagaggtt
     6421 ggccggcggc tgcaggggtc ggggatgaac atttgttaaa ataatgtaaa aaatgcttga
     6481 caaaagcaaa catatgtgct atatttaggt gaacaaatgt tctgctaacc tgatcaagag
     6541 ttcacccatg tccgacacat tgactccaaa acagcgccag gtgcttgaag ccatccggcg
     6601 attccagcag gaaaagggct atccccccac ggtgcgcgag ctcgcggagc tcttcggcca
     6661 gctctccaca gcggggatgc acaagatcct catgaccctc caggaaaagg gggtgctcag
     6721 ccgccgtgaa gggaccaagt cgcgctccta tcacatcatc ggcgaggagc cgggcgaagg
     6781 cccggccggc cgcgcccgcc cctgccccat catcggtgag gttgtggccg gcatgcccga
     6841 actggccctc gaggagcggg aaggcgatct ctatctcgac tcggactggg tgggcaagga
     6901 ggatatcttt ctgctcaggg tgcgcggcca cagcatgatc gacgccgaca tccgcgacgg
     6961 agacctgctc gccgtgcaga tcacccagag ctgccgcaac ggcgagatca tcatcgccct
     7021 gctcgacgac gaggccacgg tcaaacgctt ttacaaggag aaagaccggg tgcgcctcca
     7081 acccgaaaac cagaccatgc agcccatcta catccccatc gatgatcccg gcttccgcat
     7141 catcggcaag gtcaaggggc tgctgcgcag atatcattaa tcatcggagc cgatatggca
     7201 acccttgcag agaccattca gatcgagcag ccgcgccccc gcccccgcac ggaacttcag
     7261 gaaccggccc tgctcatgcg caccgccagc cgccgcgtca gcagctggtt caccaccctg
     7321 gaagagcgcc acaacatcac cgccggcgag gtgatggact atctcgtcca gcgagaaccc
     7381 cggatcaagg agcagctgga ccgtctctgg gcgctcgtcg actgcgacgt ccagggccgc
     7441 tatctcgaag gcgtgctcga tgagaacgat ttccgcctct ggcgccagca cctgcgcgaa
     7501 tggctcaacc tgctccactc cgccctggcc ctggtccgcc tccatcgcag cagcgatgcc
     7561 gagggggatt ttgccctcgc cgccggcgct ctcaccccgg cggcggcctg acgccccggg
     7621 gttgaaccgt tcttcgacct gacgatttgt aaaggcagtg atctatggca aaaagcatta
     7681 gaaccgattg ggccgcctac tgggacaaat ttgatgatct cgatgaactc caatccaaat
     7741 ccttcaacct cgacgacacc ctgatcgaaa ttgaaaggta tgccggctgc aaactcctca
     7801 gcggcgacca gatgcagatc atcatggccc tcgaagagcg catccgcgaa gtgagcaaac
     7861 gctctggcca gggcgctgtg agcgcccagt acagcctctt cgactgaacc cgaatttccc
     7921 cgccatcccc tcccggcgcc ccgccgggac gacgccccct ctgcggcgca gccacctgcg
     7981 ctgcgccgca gagtccccct tgcccggtcc ggcctgtgtt cgtgcgcccg cctctgcttc
     8041 cccggcgcag gccgggccgg gatgaccagc cctcgcctct ccctccagcc agccaaccta
     8101 cacccgccga ttaaaacagg ctaccccccg aaatctcgtt cccacccaaa gataattaat
     8161 taaaaaaata aatgttatct gccataaata ccttgtaaaa taaatggcct catgaagagg
     8221 gcacaggctg tgagtaggct ggaaatggta tggagggatt ggagtacgcg gcgccaggcg
     8281 tcaggtggga gccagatttc gggggtaccc cgccgattaa aaccgttgac tattttcaag
     8341 caattgccta tatttctgtt tatggttaaa ttataatagt taccgcgatg atgaccggca
     8401 tcctcccgga gcggccccct gactggcagc aagccgtcag accggccgca gccgttcccc
     8461 ggcaaagccc aagaaaggtt cgaccctcca tgagctctcg cgaactcgag aaaatctacg
     8521 atcccaccct ggtggaagac cgctggtacg ccttttggga agaaaaccac ctcttccacg
     8581 ccgatgcccg ctccgaccgg gagagctata ccatcatgat ccctccgccc aacgtgaccg
     8641 gcatgctcac catgggtcat atcctcaata acaccatcca ggacctcctc atccgctgga
     8701 aaaaaatgca gggctatgaa accctgtgga tgcccggcac cgaccacgcc ggcatcgcca
     8761 cccagaacaa ggtggaggcg gccctgcgcc aggaggggga gacccgctac accctcggcc
     8821 gcgaaaagtt cctcgagcgc gtctgggagt ggcgggaaaa gtacggtgga atcatcttcc
     8881 agcagctgcg caaactcggc gccgcctgcg actgggagag ggaatgcttc accatggacg
     8941 agggtttgag ccaggcagtg cgcgaggttt ttgtcagcct ctatgaaaaa gggctgatct
     9001 acaagggcaa ccgccttatc aactggtgcc cgagctgccg taccgccctc gccgacgagg
     9061 aggtcaattc gatcgaggag gaggggaggc tctggaacat cgcctacccc cttgcggagg
     9121 gggagggcga gatcatcgtc gccaccaccc ggcccgaaac catgctcggc gacaccgcag
     9181 tggcagtcaa ccccgccgac gagcgctatc agcacctcat cggcaaaaag gtcatcctgc
     9241 ccctggccaa tcgggccatc ccggtcgtcg ctgacgaaca cgccgatccg gccttcggca
     9301 gcggcgcggt caagatcacc ccggcccacg atttcaacga ttttgaggtc ggccagcgcc
     9361 atggcctcga gctggtgcag gtcatcggtc cggacggccg catgaacgcc gccgccgggc
     9421 tttatgaggg gctcgaccgc tttgaggcgc gtaaaaaaat cctcgccgat ctgcaggcgg
     9481 gcggcctctt gcgcggcgag atcaggaaga acatcccggt gccgcactgc taccgttccg
     9541 gtgatgtcat tgagccccat ctctcggagc agtggtacgt ccggatggcc cccctggcgg
     9601 agcccgccct cgaggcagtg cgctccggcc gtatcaaatt ctatccccgc cactgggagg
     9661 agacctattt tcactggatg accaacatcc gcgactggtg catcagccgg cagatctggt
     9721 ggggccatcg cgtgccggtc tggtactgcg accagggcca caccatcgtt cgccgcgaag
     9781 ccccggcctc ctgcccggag tgcggcagca cggcgctgcg ccaggatgag gatgtgctcg
     9841 acacctggtt tagctcctgg ctctggccct tcagcaccct gggctggccg gagaagagcg
     9901 aggagttgaa aaaattcttc ccctctgata ccctggtgac cggtccggac atcatctttt
     9961 tctgggtggc gcgcatgatc atggcctcgc tggagttcat gggagatatc cccttccggc
    10021 aggtctattt caacggcatg atccgcgacc tcgagggccg caagatgtcc aagtcgctcg
    10081 gcaactcgcc cgatcccttt tggctcatcc gcggcgccgc cgccgccgat atcggcgagt
    10141 tcagcaagct caacgccagc taccgcagcg gagtgcccgc ctacggagcg gatgccatcc
    10201 gcctcaccat ggtctatttg acccccctgg gcggggatgt ccgctttgac cataccctgg
    10261 tggagatggg ccaaaagttc gccaacaagc tctggaatgc cgcccgcttt atccttatga
    10321 acctggatga tgaggaaacc cttctgcctg tcgcagagat cccggcggtg gagatggagc
    10381 tggccgaccg ctggatcctc agccgcctgc atcgggccct ggccgaggtg gagagggctc
    10441 tgcaggagtt ccgactcaac gatgccacgc gggccgtgta tgatttcatc tggggcgagt
    10501 actgcgattg gtaccttgag ttgatcaaga agcgccttta cgatgaggcc gaccccgcag
    10561 cgaggcgggt ggcccgttcg ctggcgaccc atgtgctcga aagcagcgtg cgcatgctcc
    10621 atcccttcat gcctttcatc accgaggaga tctggcagca cctgcccgcg gagtaccagg
    10681 aggaaaagcc cgtccgcact atcatgctgc agagctaccc aaaggccgat gcggccctgc
    10741 tggatgagac agcggagcag gagatgtcag tgatgcagca ggtgatcggg gcggtgcgca
    10801 acatccgcgg ggaaatgaat gtcccgccag cccgcgaggc cgatctgatc atcaacagcg
    10861 ccgccgcggc ccccctcgag ctgatcgagc gcaatggtgg ctacctgcac aagctcgcgc
    10921 gcgtcggccg gatcctgtac aatcagccgc gcccggccgc tgcggccagc gccgtggcgc
    10981 aggggattga gatcctcgtc cccctggccg gcctgatcga tgtcgatcgt gagaggggcc
    11041 gtctcgaaaa ggagatcgcc cgtctcgagg ggcagctctt cggactgacc cagaagctga
    11101 tgaaccggga ttttatcgcc cgggccccga aagaggtgat cgagaaggag gagaagaagc
    11161 gctctgatta ctcgctcacc ctggaaaagc tgcgcgcaag cctgcagagc atcgcgggtg
    11221 aatgacacct gcagcctttg accggcaagg ggcgaggcgg ctgctccaaa ccatcaacgc
    11281 aacactatag gagattgcaa tggaacatcc attctggctg gtcctgtttg tcaccctgct
    11341 gccactgctg gccctggcgg cggagccgaa aatcgccgtc accgtctata acgaggattt
    11401 ggccctggtg cgtgaggcgc gcagcctgaa gctggtcaag gggagccagt cctatgccta
    11461 cagcggcgtg gcggcccaga tcgacgccac cacggtccat tttcgcgccg tcgatccatc
    11521 ggccggcatc accctgctcg agcaggacta tgagttcgat ctggtgggaa ccgagaggct
    11581 gctgcaaaag tatattgatg agcaggtgat cgcgaccgcg gagaacgggg cggttcacag
    11641 cggcaagctg ctcagcgccc aggatgggga tatcgtcctg ctggacagcg gcggcaaggt
    11701 gcaggccctc aaggccgcca agctgcagag cgtcgagttc ccctcgttgc ccgaggggct
    11761 gcgcacgaga ccgaccctgg tctggctgct cgagagcagc aaggccggcg atcatgagac
    11821 cgagatcagc tatctgaccg gcggcctgag ctggcacgcc gaatatgtcg ccgtgctcga
    11881 ggcggcggag aacgccgccc agctcaacgg ctgggtctcg atcgacaatc agtcgggcgc
    11941 gacctacact gacgcccggg tcaaactggt cgccggtgac gtcaaccgca tccagccgga
    12001 gccgcaggtg cgcgccatgt tccgcaaaga agcagccatg gcgatggatt cccagttcga
    12061 ggagaaggcc cttttcgagt atcacctcta taccctgcag cgtccggcca ccctcaagga
    12121 caagcagatc aaacagctct cgctctttcc ggccgcgcac accccggtga ccaagctcta
    12181 ttcgtacgac ggggctttcg acggcagcaa ggtcaaggtc aaactcgagt tcaaaaacag
    12241 ccaggccaac ggattgggca tgcccctgcc caagggtgtg gtgcgcgtct acaagcagga
    12301 taccgacggc gcccaggagt tcattggcga ggatcgcctc gaccacactc ccaaggatga
    12361 aaaggtgcgc ctcagcctcg gcaatgcctt cgacatcgtc ggcgagcgca tcgtcaagga
    12421 tgtgcgcgaa gtctcccgca gctcccgtca ggagacggtg cagatcaaac tgcgcaatca
    12481 caagagcgaa gaggtggtcg tcaacgtcat cgaacatttt ggcggcgact ggagcttcat
    12541 cggcaagacg ccgaccatcg ccgtcaagga ggcgggcaag gccgagttcc aggtgcgcgt
    12601 gccggccggc gaagaaacgg cattcgagta tacggtgctc tacaaatgag gcccggtcgg
    12661 gcggtctgag cgagggagcc ggtgaacacg agccccaaaa ctgccctgga gtggcctgtc
    12721 gaccgcattc cgggcattgg ccagaagcgg gcaaccgccc tgcagaatgc aggcctctat
    12781 acgatcggcg acctggtcta ctatttcccg cggcgttatc tcgaccgatc gacagtgacc
    12841 cggatcaacc gcctcaagcc tgaccaggag gtgaccgtag tcgccagggt gatccagttc
    12901 actgtgcaaa aggggcggcg cagccgcttc gtccttgtcg tcggcgatgg caccggtttt
    12961 ctgcattgca tctggttctc gcggcttccc tactggtaca aggccttcaa accgggcgag
    13021 tggctcgccc tgagcggcaa ggtgggcttt ttcaagggac tgcagatggt tcatccggag
    13081 ttcgaccgcc tcggtgtgac cggggagggc gagcaggtcc acaccggcaa gatcatcccg
    13141 ctctacccct cgagcgagcc ccttggccag gcgggctttg acagccgcgg cttccgccgc
    13201 attctgcaca cagtgctgaa gaactggggc gatcagatcg acgagaccat ccccgcggag
    13261 ctggcggagc ggctggggct ggcgcccctg cgccaggcgc tctcggccat ccatttcccg
    13321 gccgattttg ccaccctcca ggccgcccgg atgcgcctca agtttgacga gctctttttc
    13381 atggagcttt tagtggcgct gcgccgccgg catcagcagg agggcacagg ggggatccgc
    13441 tttgaaaagg tgggcgaact cacccggcgg ctggtcgaat ccctgccttt cgccctcacc
    13501 ggcgcccaga agcgggtgct gcgccagatc cgcgccgaca tgaagagccc gcggcccatg
    13561 caccggctgc tgcagggcga tgtcggttcg ggcaagacgg tggttgccct catcaccatg
    13621 ctgatcgccg tcgagaacgg ctatcaggcc gccctgatgg ccccgaccga gatcctggcc
    13681 gagcagcact atctcacgat cagggagatg ctgcgggagc tcgaggtcga ggtggtcctg
    13741 ctggtcgggg ggcagggacg gagcgagcgc ggggaactgc tcgaccgcat cgcctccgga
    13801 gaggcacgca tcatcatcgg cacccacgcc ctgatccagg agtcggtcga ttttcatcag
    13861 ctcggtctgg tggtgatcga cgaacagcac cgattcgggg tgatgcagcg ggcgacgctg
    13921 cgggagaagg ggctcaatcc cgatctgctg gtgatgacgg ccacaccgat accgcgcacc
    13981 ctgaccatga cggtttatgg cgacctcgat gtctccatca tcgatgagct gccagccaac
    14041 cgcaagcccg tcaagacctt ctggcgggac gaaagccggc gcagcaagat ctaccatttt
    14101 ctgcgcagcc gggtgagtga gggggcgcag gccttcatcg tttttcccct ggtcgaggag
    14161 tcggagaaat cggacctcaa ggccgcggtc gagagctatg aggcgatgag cgccactttt
    14221 tttaaggatt tccgcatcgg cctcatccac ggccggatga agccggagga gaaagaggca
    14281 accatggcgg ctttcaaacg ccatgagctg cagatcctgg tcagcaccac ggtcatcgag
    14341 gtcggggttg atgtgcccaa cgcctcgatc atggtggtcg agcacgccga gcgcttcggg
    14401 ctgacccagc tgcatcagct gcgcggccgc gtcggccgcg gctcccggca gtccttctgc
    14461 attcttgtca gctacggcgc tttgaccgaa gaggcgcgaa cgcggctcga gaccctggcg
    14521 gtaaccagcg atggcttcaa gatcgccgag gtcgacctgc agctgcgcgg cccgggtgaa
    14581 ttcttcggca cccgtcagca cggcctgccg gagctcaggc tggctgatcc cagccgcgat
    14641 ctagaactgc tgcgcctggc gcgcgacgag gcctttcgcc tggtcgaggc cgatccgcag
    14701 ctgcgcgatc aggagagcct gggcacacgt cattattttg tcagaaacta tcaggagcgc
    14761 tttggactgg cctgggtcgg atgaccgcag ccgccggccg ctcccggtcg ctaaccgcaa
    14821 aatcaggaac agctatggaa gaacagaaca aacaccatgc cgcactcctc ttcaatctgg
    14881 tcatgatgtt tcattccgcg gcgatgcagc agatgggcaa actgaagaac ccgatgacca
    14941 atgccatcga acgggatatg gaacaggcgc gcctctccat cgacatgctc gacatgctcc
    15001 aggccaagac ggcgggcaat ctcagtgagg atgagagcaa attcatcggc cacgtcatca
    15061 gcgagctcaa gctcaacttc gttgatgagg tgaacaagga caagcaaaag gcggcggaga
    15121 gcgccaccgc cggcgagacc cctgctgaag gaggcacggg cgaaagtgcg ccagaaaacc
    15181 gcacagctga atgagcagga acgacaaaga aagatgagcc aaaagacgac gctgcaccgg
    15241 cgcgaggtct gggaccagtt ctggcaggag cgggaggaga tcgagcaggt ctattccaat
    15301 tccgagcgag ttatcgatca gctcatccgc cttggcgatc cggccggcaa gtggatcctc
    15361 gaagtggggg ccggctcggg ccgcgacagc ctcgccctga gcagccgggg cgggcgggtg
    15421 atcatgctcg actattcggc ccccgccctg gaggtcatcc agaaactggc ccagaagaca
    15481 ggccgaccgg tctatctggt gcgcggtgac gcctttcacc tccccttccg cagcgatgcc
    15541 ctcgacctgg tctttcacca ggggctgctg gagcatttca ctgctcctga agggattctg
    15601 gccgagaatc tgcgcgtgct ccggcccggc ggggcggccc tggccgatgt gcctcagcgc
    15661 tatcatcttt ataccgcggt caagcatctt ctcatcgcct tcaaccgctg gtttgcgggt
    15721 tgggagaccg agttcagcca tgcccggctt gagcgcctct tcgcgcagac cggtttcgtc
    15781 cgcaccgagt tttacgggga gtggatgcgc cccagcttca tctaccgcgc cagccgggag
    15841 gcgctgaaaa aggcagggat aaccctgccc ctttatccgc cctccctgcc cctgttcggg
    15901 cggatccgcg ccggtctgcg cgccctgctt cgccgccatc cctggcacct caacaccgtc
    15961 atggatatcg gcgccatcgg cttcaagccg gtcggctcgg accaaagggc cgggtgagcc
    16021 ccttgcgcat cctggtcata aactggcagg acatccgcaa cccgctcagc gggggggcgg
    16081 aggtccattt tcatgaaatc ttccgccgcg tcgcggcgat gggccatgcg gtcaccctgc
    16141 tctgctccgc cttcgccggg gcagcagcgg aggaggaaat cgacgggatc cggatcgtgc
    16201 gctgcggttc gcgccagctt ttcaattttg ccgtcccggc cgcctatcgg cgcctgcgca
    16261 aggcacaggc gttcgatgtg gtcatcgatg atctgaacaa gattcccttc tacaccccct
    16321 gcttcgtgca ggagcccttg ctcaccatcg tgcaccacct gttcggccgc agcatctatc
    16381 tggagacctc ctggcttccg gcaagctatg tctactggtc ggagcggctg gccctgcgca
    16441 tctatcgccg gactcccttc gcggtggtct cggagagcac ccgggatgag ctgcgcggcc
    16501 tggggatcac cgccccggtc gagctgctgc ccaatgcggt cgaccatgag cgttatccat
    16561 acgcgcctgt ggagaggagc ccggagccgc tgatctgtca tctcggccgg ctcaagcgct
    16621 ataaaagtgt cgagcacctg ctgcgggcgc tgccccgggt gctcagttcg ctgccggaca
    16681 cccggctggt catcgtcggg gaaggggacc atcgtccggc cctcgaagcc ctggcggggg
    16741 aactggggat aggtggggcg gtcaccttca ccggccaggt cagccatgag gagaaggtac
    16801 actggctgaa ccgcgcctgg gtggcggtga atccctcccc caaggagggg tggggactga
    16861 ccgtcatcga ggccaatgcc tgcggtgtgc cggtggtagc cgccgactct cccgggctgc
    16921 gggattcggt gcgtgaccgg gagaccgggc tgctctatcc ctacggggag atcgaagccc
    16981 tggccggggg gctcatcacc ctcctgtgcg accggacgtt gagggagagg atgggtgaag
    17041 cggcacggct ctgggccggc accttcagct gggaccagtc cgcccgcaag gccgtggcta
    17101 ttctcgaggg catgggactc cgcagccagc ccggcggacc ggccgcggtg ggtaaaaatt
    17161 gagatcattg gaacgcattt tacgcaagtg gttaacctaa cggagtggag tcatgcaaaa
    17221 attcaagctc tataaccgca tcgccgccgc cgccgctttt ctgctctccc tggtggtcta
    17281 tctgcgcacc atcgcgccga cggtttcctt ctgggattgc ggtgaattca tcgcctgcag
    17341 ccatcttctg gccgtgcccc atccccctgg cgctcccttt tacctgctcc tcgggcgtct
    17401 ggccagcatg attccatgga cgggagagat cgccctgcgc gttaacctga tgtcggcgct
    17461 ggtcacctcc ctgaccatca tgctcaccta tctgatcatc gtcctgctca tcaagcagtg
    17521 gcggggcgaa cccagggacg gcgcggatgc cttcatcctc atcgcctccg ggctgctcgg
    17581 ggccctggcc ctcgccgtct ccgatacggt ctggttcaat gcggtcgagg cggaggtcta
    17641 cgccttttcc ctggccctga cggccgcggt cttttatctc gccctggtct ggctggagaa
    17701 atccgaccag gttggcgcgg agcgttatct gctcatcatc gcctatctca tcggcctctc
    17761 gctgggtgtg catcttttgg cggttcttgc cgtgccggcc atcgccctgg tgatctattt
    17821 caaatggctc aagatcaggg acaaaaagtt caccttcggc agcctgttgg ccttcatggg
    17881 catcgcactg ctgatcacgg tgcttatcta tcccggcacg gtcaaactca ttccggccct
    17941 ggccggtggt ctcggcctct gggcggtgtt gctgctctac gtcctggtcc tcgccggggc
    18001 ctggtgggcc gtcagcaacc gcaaccgcat ggccagcctg gtcttcatga gcgcttttct
    18061 gatcctgctc ggctactcca cctacaccat gatcctcatc cgctccggcc tcgatccggt
    18121 catcgacgaa aacgatcccg aaaccgccga gggtatggta aagtacctca accgcgagca
    18181 gtatggggat tggggcacct tcccgcggcg cttccccggc ctgccccagg agtggcagtt
    18241 cgaccaggaa cacccgaacg agaactacaa gacctggcag tggtccaagc agatggattt
    18301 tctttgggac tatcagatca aaaagatgta tctgcgctat tttggctggc agttcatcgg
    18361 caagggggat acccggggcg cggacggatt catcgccgag aatttcacct ggcggggact
    18421 cggtatgctg ccctttctga tcggactgat cggcatggtc taccacttcc gcaaggattg
    18481 gcgtcactcc tcctcgattc tgctcctctt tctcgccacc ggcgtggcca tcgtcctcta
    18541 tctcaatcag gaaaaccccc agccgcgcga gagggactat gtattcgtcg gctccttctt
    18601 cgccttcgcc ctctggatcg gcatcggcgc ctcggccctc ttcgaatgga tcaaggagcg
    18661 ctattacctg atcgccggca atcaggagcg gggagcacaa aaaaacatgc tctacaaggg
    18721 actcaccggc ctgaccttgc tgctgctcat cctcgccctg ccggtcaacc tcttcagctt
    18781 taatttcagg gatcatgacc gtagcggcaa ttatgtcgcc tatgactatt cctacaatat
    18841 cctgcagtcc tgcgaaccca acagcatcct tttcaccaac ggcgacaacg ataccttccc
    18901 gctctggttc ctgcagtatg tctacaacat ccgccgggat gtccgcgtcg tcaacctgag
    18961 cctgctcaac accgactggt acatcaagca gctcaagaat gaggagccgc gagtgccgat
    19021 cagcctcacc gacaatcaga tcgggcagct gtcgctgatc ccctgggagg cgcaaaagat
    19081 caagatcccg gttccggcga acgcccgctc cgaggtgctg ggcgagatcg atgtcgcctc
    19141 cacggcccac gctgagcccc tgaacgagct ggaggtaaac gtccagccca cgatcatggg
    19201 ccgggccctg cgggttcagg atttcatgat cctcaacatc atctacgcca accagtggaa
    19261 aaagccgatc aatttcgcag tgacggtttc ggatgacaac aaggtcaacc tcgatccctg
    19321 gctgcgtatg gatggcctcg ccttcaagct ggtgcccatg gatggagagg tcctggcgcg
    19381 cgaacggctg cgcaagaacc tcttcgagga tttcaaatat cgcggcctcg atgacaaatc
    19441 ggtctactac gatgacaacg tcatcggtct gctgcagaac taccgcgccg gcttcctgcg
    19501 cctcgcccag gaatacctga tgcagcgcaa ccatgacaag gtgatcgaga ccctcgacag
    19561 gatggaaaag gtgatcccgg aaaacgtgat cccggtcccc gattaccgcc tctcgctgcg
    19621 cattggccag ctctacgatc tggcgggcaa gagcgaggag ttcatccgcc gcgccgagga
    19681 ggcgatcaag gccaaaccgg atgatcccat ggtctacggc tatctggtcg cgaccctgag
    19741 ccgctccggc aatcacaagc gggcggtgga gctgctcgag gagtggctgc agcgccatcc
    19801 ggaggatcgg gaagcagcca gccggctgac cgaggagcgc gccctgctca gcagcgggcg
    19861 ctagaacgaa tcaaggaagc gggcgctcgg gattattgcc ccaggcgccc cggatgaagc
    19921 atcaggcgcc ggagcggatt ccggtgctgg caagggagca ctcggaacaa agcgtgtcaa
    19981 gaaagaagca tcatagcagc cgacagctct ggcggccccc ggcggtcacc ctgctcaaga
    20041 tcgccgtcag cgccaccctg atcatcctgc tcctgcgggg gataggttgg gctccgatca
    20101 gggaccagtt cagtcacgcc ctgtggggga gcgccgccgc cagtctgggg ctctttgtgg
    20161 tcagcaattt cctcggggcc tggcagtggc atctattgct ggagagcaag aagatccatc
    20221 tttcttttcg ccgggtcctg ggctattata acatcgggct ctttttcaac aattttctca
    20281 tcggcaatgt cggcggcgac gccttccggg tctacgatat ccggcgcatc tccggggaga
    20341 ccaacggggc cttgtcgacg gtgtttttcg accgctttgt gggttttttc acaatggctt
    20401 ccctggcact cttcgcgacc ctgatggcag cgcggcagat ggtctcggcc tcggcgctgg
    20461 aggcgatcct gttcatcttt ggggcctggg ctctggcgag cgcctttctc ctctatgagc
    20521 cctttgccaa gaaattttca tggattttcc ggctgctgct gccggcctcg ctgcacgtca
    20581 aggcgaaggc cttttattac agcctgcatg aattccgcag ccagaaaagg ctgctgctgc
    20641 acctgctctg catttcgctg ctggtgcaga ccttgcggat aatgacccat tttttcgcag
    20701 cccgcagcct gggcgtgcgg gagcgggcca gttttttctt tctcttcatt ccggtcgttg
    20761 ctctggtggc gagtatgccg atctcgctcg ggggcctggg ggtgcgggag cagagtgccg
    20821 tcaccctctt cagtccgctc ggcatccctg cggccaagat cgtcgccttt gagtttctcg
    20881 cctacctggt gggggtcgtc tcgagcctgc ccgggggcat ccttttcgct ctgcggcgtg
    20941 aaagccgcac gggagcctag cacccggctg ctccgctgca tgaggccggc ctgcaaaaga
    21001 tccagtaaaa gaggacgata tgtataaagc agccatcatg atgggctcgg aatccgaccg
    21061 cgaggtgatg tccgaggccc gcgcctatct cgactatttc ggcattgatt atgagatcaa
    21121 gatactctcc gcccatcgca tgccggacgc gaccgccgaa ttcgccaaat cggctgaaga
    21181 gaacggtttt tgtgccatca tcgctggggc gggcatggcc gcgcatctcg ccggggtggt
    21241 ggccagccac accctgctgc cggtcatcgg cgttcccctt tcgggctcgg ccctgaccgg
    21301 tgtagatgcc ctctattcga tggtgcagat gcccaggggt gtgccggtgg ccacgatggc
    21361 gatcggccgc gccggtgccg ccaacgccgc catctttgtc gctgaaatgc tcgccggccg
    21421 cgacgccggc atccgcgaca agctggtcga atttcgcaaa aagggctgtg tgctgtgaga
    21481 agagtatgcg gatactgatc accggcagca acggcctgct cgggcagaac ctgagccgcc
    21541 tggccccggc cggcgccgaa ttgatgggaa tcgatctgca ggagatggtg gaggcccccg
    21601 ggctgagccg atttcgccgc cttgacctgg gcgagcgcaa gctcctgctc gcctgcgtgg
    21661 aggagttcgc ccccgactgg atcctcaacg ccgcggcctt taccaacgtg aacggggccg
    21721 agagcgcgcg cgaactctgc tggcgcgcca atgtgaccgc agtcgaaaac ctcgccctcg
    21781 cctgccgcaa gctgggcagc cgcctggtcc acctctccac cgactatatc ttcgatggcg
    21841 accacggccc ctatcgtgag gaggatctgc ccaatccgat cggctattat ggcaaatcca
    21901 aactggcctc ggaaaatgtc ctgcgcagct cggagatccc ctatacggtt gttcgcacca
    21961 tggtgctcta cggccatgcc cgtcaggtta aacccgattt cgtctcctgg ctgatcagtt
    22021 ccctgcggca gggcaagccg gtgcgcatcg tcactgacca gtttggcaat accacactgg
    22081 ccgaggaact cgccctcgga atctggcgga tcgtcgagcg ccgggcgacg ggatttttcc
    22141 acattgccgg caccgagatc atcgaccgct acgctttcgc cctcaaggca gcggaggttt
    22201 tcaatctgga ggcgggcctg atcaccccga tcaccaccgc ggagctgcat caggaggccc
    22261 cgcgtccgat gcggtcggga ctggtcgtgg aaaaagccct gcaggaactg gagctggaat
    22321 tgagcgacgc ggcggggggg ctgaggaaac tgaaacagca gctggatgcg cccacttgag
    22381 cctgaggagc agatcgtgaa aactctcgtc atctttccca cctataacga gaagcagaat
    22441 atccttgcca tcatcgaggc cgttttcgcg cagaatatcg aggggctgga gattctcgtc
    22501 gtcgacgaca actcgcccga cggcaccggg gatctcgtcg ccggacggac cgggatggag
    22561 ccgcgcctcc atcttctgcg ccgcgaccgc aagatgggat tgggcaccgc ctatgtcgcc
    22621 ggtttccgct atgcgctcga gcgcgattac gatcgcatca tcgagatgga tgccgatttc
    22681 tcgcacgatc ccagggacgt ggtccgcctg ctcgaggcga gcgctgacag cgacatcgtc
    22741 atcggctcac gctatatcaa gggggtcaac gtcgtcaact ggcccctcaa acggctgctg
    22801 ctgagcatgt ttgccagcca ttatacgcgc attatcacgg ggctgccgct gcgcgattgc
    22861 accgccggat tcaagtgctt ccgcagggag gtgctcgagg cgatcgaact ggacagcatc
    22921 cagtcggatg gctactcttt tcagatcgag atgaatttcc gagcctgggt gaagggcttt
    22981 cgcattctgg agatcccgat catctttgtc gatcgcgctg ccgggacctc caagatgtcc
    23041 aagcgcatcc ttcgcgaggc agtctggatg gtctggaagc tgaagggatt gaagattctg
    23101 aacaggcttt gatcagcggg cgcgggtcat gcgcagcatc ccatcccggg tatcgagcag
    23161 aggagtgcgg aggagggcgg cggcgcggct gctgtccagg gagacatcga ccgggcgcgg
    23221 ggcgtcgctg accgcagtgt gcatcgacac cggctgaagc agcgcggggt catagcc
//
LOCUS       DAOPWU010000015        22959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_2235_length_22959_cov_5.401415, whole genome shotgun sequence.
ACCESSION   DAOPWU010000015 DAOPWU010000000
VERSION     DAOPWU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22959
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_22959_cov_5.401415"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKM23_01895"
     CDS             complement(<1..704)
                     /locus_tag="PKM23_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90254.1"
                     /translation="MADDIGPILRKWHYSANDMNVRLIQGEDGRQKLQLRLDLGLLQM
                     ELDGRPDGRRPHHFESYYSYYLHKSRLAEKEEARSLNLTPFDCWLLQQEAIQLYHRYV
                     ALMRLGDFGRVLRDTKRNLQLFDFVGSHSSNETIIWSFEQYRPYVIMMNTRAAVSQCM
                     EFQDYDKALEQIRKGRSRLRRFYRMHGEKAGDERVELELLQEWAVEIRSKRPISDRDQ
                     LARELRRAVRSEDYERA"
     gene            complement(753..1529)
                     /gene="purQ"
                     /locus_tag="PKM23_01900"
     CDS             complement(753..1529)
                     /gene="purQ"
                     /locus_tag="PKM23_01900"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HNY90255.1"
                     /translation="MTKPRVLVLRAAGSNCDMETHHAFEYAGARAEMIHINRLLRQEV
                     RLADYQILAIPGGFTYGDDISAGKILANELRYKLSQELAGFHQSGKLIIGICNGFQVL
                     VKAGLLPELDLDAGQKVTLVHNDSGKFEDRWVYLKVHESPSVFTTPMPEQVYFPVAHA
                     EGKFAVSYPAVLERLEANHQIVFRYCAADGGSAAYPANPNGSVRDIAGICDPTGRILG
                     LMPHPERHIDPTNHPRWMRDGAAREGDGVAIFRNAVAYFR"
     gene            complement(1534..4395)
                     /gene="purL"
                     /locus_tag="PKM23_01905"
     CDS             complement(1534..4395)
                     /gene="purL"
                     /locus_tag="PKM23_01905"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNY90256.1"
                     /translation="MITRIELAQKEQYHDAEGKAILAGARDLGVRALTAVHAFRVYYF
                     LGELTPAEQELIAVRLLADAVTDDWIINALLQRSRGEFWSAEVALNRGVTDMVAESAR
                     KGAIDLGLRAVEAVRTARRYELCGSLSAAEKELIAQKLLMNKVVEHLHQPGERLFFPA
                     EEPPFELIHVSLLQASGEELMKISRERDLFLNLTEMETIQAYFRSLQREPTDVELEMI
                     AQTWSEHCSHKTLTGLVEFEGRVIDNPLKETIFRVTRELNLPWCVSVFKDNAGIIEFD
                     DDYNVCFKVETHNHPSAMEPYGGANTGIGGVIRDPMGTGLGAKPIINTDIFCFGPVDL
                     PHDQLPRGVLHPKRVMQGVVAGVRDYGNRMGIPTSNGALYFDERYLGNPLVYCGNVGL
                     IPRDKCEKQVVPGDYIVVVGGRTGRDGIHGATASSGELHSESEGTWSGAVQIGNPIVE
                     KKLVDTLMQARDLGLYRAITDCGAGGLSSAVGELAKTTGAEVDLEFIPLKYHGLTYME
                     IWISEAQERMVIFVAPDKLEQALAVFAAEDVEATVIGRTTTDHKIRLRYRGQVVGELE
                     MAFLHDGQPRIVRKARWDPPAIAESEGAQPASLGEILHRILAAPNVCSKEWVIRQYDH
                     EVQGGSVLKPLVGVVNDGPGDGCITRPRLESHRAVILANGLNPKYGLIDPYWMAASAI
                     DEALRNIIAVGGRLERCALLDNFCWGNTDKPDRLGGLLRAAQACYDMALVLGTPFISG
                     KDSLNNEYKTESGESIAIPPTLLISAIAVMEDCRTAVSMDLKRPGNALYILGETGKEL
                     GGSHYYEAIGHSSSAVPRVDPARARALFIALADAIAAGLVRACHDCSEGGLGAALAEM
                     AFAGGLGAEVTLCDLPRSADLLRSDWALFSESNSRFIVEVIPGKEEEFRARFMQLPCA
                     RIGEVTSGPRLRIRGLIGELVVDEAIDLLKNSWQETMRW"
     gene            4766..5881
                     /locus_tag="PKM23_01910"
     CDS             4766..5881
                     /locus_tag="PKM23_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNY90257.1"
                     /translation="MAAARRAIRRHRASPVGFIALLLILRAATPLGLQALPSDSTSAP
                     AGAIQVFNPDRAPARSAADIAAEIIAAETAIYALRTASPITFKSDSTAPEIIPELAIQ
                     RLRLELGQKIQAISGPDSTFYQPFAGLRSGAHDESSAGPVHRMQEALLRWNNKLPLKT
                     NGHYDEATIKSLTLFKLVYDLGHDGSFVDQATAGYLLAIEEGNDQELAEPQGFTGRLV
                     YHAAQFLGLRYRLGGDGKKTTDCGMLTRMAMISAGLVDEVFNRVAATQYKYAEEGKMG
                     LTLVDKNEEPAPGDLVFFKWNTRRARHRYKGITHVGIFLGKVGESLYVLEAASARKTR
                     KVTILDRSNSIRKIAGYARFTAPPEDGPIEPALEMTD"
     gene            complement(6005..7387)
                     /locus_tag="PKM23_01915"
     CDS             complement(6005..7387)
                     /locus_tag="PKM23_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNY90258.1"
                     /translation="MVRRAVMLILLTWGAAMVRAQSSYPDQHFVIESGDSLLAHAESL
                     DRVVLSADRKSLRLEDGATSGFLILKPWQAPWPFDEGLPSWNGTAPGDSGGFRVLVRF
                     PYLSGWSPWLDAGFWKANLWPGKKTTFAGGEIDIDIARLNYYASAWQFAVEFRRSTAA
                     VTSPSIALLSWFASDSRTTTGLDYAAILRDKPEAIFVQTSFLAQYRIDPEIGGSICSP
                     TTVAMILLSFNIAVDPLQFARDTRDPYYGLFGVWPRVVQNASEYGVKGTVTRYRSWGE
                     AREVLARGGRIGMSIGAPLYSGHLVMLAGFTANGDPIVHDPARTNTGYGYIYDKNDLS
                     HAWFDKGGVAYTIFMPDSTASGVEAPAAGREGPAAFVLRQNWPNPFNASTTFEYELSR
                     EGYVEFHIFDLLGRRIITLAEGRQPAGLHRLRWEGRDHSGMSAASGVYLYQLRVEGGE
                     VRTGRLTLIR"
     gene            complement(7389..8813)
                     /locus_tag="PKM23_01920"
     CDS             complement(7389..8813)
                     /locus_tag="PKM23_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNY90259.1"
                     /translation="MNNAREIGEARVSALLIRFSVPAIVGMVVNALYNVVDRIFIGQG
                     VGSLGIAGATVGFPIMQVQMAFGMLIGLGGNALVSIRLGEQRREEAEKVLGNALVLIT
                     AISLLLTIPSLIFLRPLLRLFGASPVSMPYAVEYMQVIILGTVFAGVGFGLNHFIRGE
                     GNPRMAMKTMFIGAGLNTLLDPLFIFVFHMGVRGAAIATVISQMASMTWVLGYFFGRG
                     SLLKIRPANLRLDRAIVKKIAAIGSAPFAMHIVASLYAVLLNNQLQRFGGDLSISVMG
                     ILNSIAMLFLMPIFGINQGSQPIIGYNYGARKYDRVRTTVRLAAAAASVVAWTGFLVV
                     MFFPAPLMRLFNPRDAGLIAMGTHAMRIFFMVMPVIGFQVIGSSYFQAVGKPKQAMFL
                     ALSRQLIFIIPMLAILPRFYGLDGIWMVSPVSDLLSVLVTAFFLWYEIRQLGRKHEAG
                     LGADPGAAGPVSIPFKEEVDKVGD"
     regulatory      complement(8802..8927)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8965..10839)
                     /locus_tag="PKM23_01925"
     CDS             complement(8965..10839)
                     /locus_tag="PKM23_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90260.1"
                     /translation="MRRISAWLVLLMTAAGAQGQTMSAPFSADSTLRLGSGYGSAGDL
                     FALLPGACYQDRGSVGLPALGALFAAPPNRLNVIYDGFRLDDPLTGLSDLNLIPVESM
                     ASQVLFADPLTRPFPAWPASQTLEVTGRDLAADTLRTQVAYRSGGNGYDDIDVRAGLR
                     YSQRLWINGGGVLKNYAGATAQLEKYHAQKINLALTRTFGSLWRARYRLLYNISDLNL
                     PLPEAPPAAPDLRQPHQKLSRYDHGLEIDYAGSWRTLVQLTDQHRERYGYRHSLWDET
                     TDVLRFDLTSTWLWRRGSLSGTTGGTFRSTGLKSPAWGDHSRSLCSGWGSLILHPAPR
                     LMAFTQLRLEKEEGMGAALLPQLQLRYDLGPSPQTSARPDSTATGDAGPPLDSALSST
                     ASSPARWQSLLWYERSRREASLAERYETGPFALGDPELQAESGDHLGLGLSGHAPAWK
                     LLLFLSASRTREELMLVQEAPQTAALYRNQPGQLRFSIDLAGEWRLSSWFTLAGKAKQ
                     MLFEEQAPLNQSATCAAGWLGVRHIFFHKDLDVRLRLGSFFWGEREGPLPWYVDASKE
                     SEPLAAAAVPWLDLSVVIRDATLFFALQNPLDIDYEVVRGYPQPRRLIRWGFVWNFYD
                     "
     gene            complement(10850..11395)
                     /locus_tag="PKM23_01930"
     CDS             complement(10850..11395)
                     /locus_tag="PKM23_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90261.1"
                     /translation="MQHRAGYYNWLLAALLVALSVTVGYLFIAIPNVEMFTAAVFVSG
                     FLTGPGRGALIGAAAALLFSLFNPYGAAAPPLLLTQIISMALTGLAGGLVQRAHWLER
                     PVWIRTALFGGAGLLLTLIYDVLTTLSFAIIMAGGDAAKVWASFVYGMAFYGLHMISN
                     TLIFALLLPLLLERLRFIITR"
     gene            11611..11865
                     /locus_tag="PKM23_01935"
     CDS             11611..11865
                     /locus_tag="PKM23_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90262.1"
                     /translation="MHFETVNESVEVISYFDGRKMRPIRFRWRSRPYHITRINAIWSE
                     LQGRDCLYHFHVTTRESGSFELIYQNSGLTWRLGRVGVEE"
     gene            complement(11931..13460)
                     /locus_tag="PKM23_01940"
     CDS             complement(11931..13460)
                     /locus_tag="PKM23_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY90263.1"
                     /translation="MTLSEKIGKYRWTICALVFFATTVNYLDRQVLSLLKDRLEVEFN
                     WSDSDYANIVAVFQFVYAISMLFAGRLIDWLGTKLGYALSLLIWSLGAMIHALARGTG
                     GFMFARGVLGFGESGNFPAAIKTIAEWFPKKERALATGLFNSGANVGAILAPLTVPWL
                     ANKLGWQAAFLVVGAIGLIWLIFWFWLYETPRLSKRVKPAELAFIHSDADDSAPTQSG
                     SAVKEKISWFQLLKYRQTWAFSFGKFMTDGVWWFFLFWLPAYLKAQYGMTGTTVAFPL
                     AVLYSMSTIGSIAGGALPMYFINKGQEVYSARMKAMLLIALVPLLVLLAQPLGGISYW
                     LPVLIIGIGTAAHQAWSANIFTTVSDMFPKKAVASVTGIGGMAGGIGGIVINKIAGWL
                     FDGYRSAGIAKSFVEAQAANLGGFLDKIKSLSLVNKHGDIINLDRVELGSLPHEVMAK
                     LQAIDPAAFESLRALQVPIVQDKMTFAYTMVFAFCALAYLLAWGVMHFLVPRFKKIED
                     L"
     gene            complement(13491..14933)
                     /locus_tag="PKM23_01945"
     CDS             complement(13491..14933)
                     /locus_tag="PKM23_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HNY90264.1"
                     /translation="MMIALAVTLMLSAACVTRKNEQVKAAPAGGADPAAVRIYQTAQA
                     NGNRLADLGAAAFEPFGQPLETQPCIFIDPAHTFQTLVGIGGALTDASAETFFSLSEE
                     KQQELLTAYFDPHKGIGYSLARTNIQSCDFSSGSYDYVKPGDLELKSFNIDHDRQFKL
                     PFIKKATAAAGGALTLYASPWSPPAWMKTNNNMLRGGKLKPECFEAWAGCYIKFVQSY
                     EQEGLPMWGLSVQNEPMATQTWESCIYTDEEERDFVKNHLGPALARAGMGGKKLIIWD
                     HNRDLLYQRASTVLNDPEAARYVWGVGFHWYMNDAFENVKRVQEAFPEANLIFTEGCN
                     FPYKTENLGQWHWGENYGKSLVNDFNNGAVGWTDWNVLLDETGGPNHVGNFCFAPVHK
                     TAEGDLYYMNSFYYLGHFSKFIRPGARRIIASSNRDKLMTTAFVNPDGRIAVVVLNLS
                     DEKFACKLWLKGKAASVESLPHSIMTLVIA"
     gene            complement(14939..15964)
                     /gene="amrS"
                     /locus_tag="PKM23_01950"
     CDS             complement(14939..15964)
                     /gene="amrS"
                     /locus_tag="PKM23_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNY90265.1"
                     /translation="MHPALHACKLPGDQVQCLLCPHACILAPGRSGLCGVRRNDGGEL
                     TSLNYGKAVAVHVDPIEKKPLYHLYPGSRSLSVAAAGCNFHCRFCQNHEISQIKGGSG
                     TVPGRPVTPRQLVDQALADDCRTISCTYTEPTVFYEYALEICRLAREQGIDTVWVTNG
                     YISEAPLRELAPFLTAANVDLKGWNDEFYRSVIGGTLQPVLDTLRLMKELGIWVEVTT
                     LIVPGQVDGEDELREVARFICKELGAETPWHISRFYPQYRFTEVSPTPLAVLQRARKI
                     GLTAGLRYVYTGNVPGEIGENSYCHRCGALLIGRYGFQIVANNLQAGSCPACGTGMDG
                     LFIKEEM"
     gene            complement(15971..16915)
                     /locus_tag="PKM23_01955"
     CDS             complement(15971..16915)
                     /locus_tag="PKM23_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNY90266.1"
                     /translation="MAMKTPRVLLLGLLMTGLGGAQTPLIPTYLKQDAGLAAGLEGIV
                     SELALEGSFDTGEDGFEQISLAVIDLTGKKPRLGGVQMDNFIYPASVYKMYVAAEILH
                     QVSQGRLSLEQSHIIAGHNAVDRSKEWTSDPRPLLQARDTVTVNYLLDLMITRSDNSA
                     ANCLIDLATRRSIDSLMHTYGWQGSEVTRKFLKRRFEDPGYEAVRGTETCALHAADFL
                     CRIATGRLVNPWVSMQMMCLLGRQLDNAKLAVGLPPGAMFYHKTGWYGSWSHDAGIVD
                     DGQVRYVVACFLPLPEEKAAAKHRQLAEKIHTLMRARR"
     gene            17167..18273
                     /locus_tag="PKM23_01960"
     CDS             17167..18273
                     /locus_tag="PKM23_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNY90267.1"
                     /translation="MIYHPYGTTGLQLSAIGFGGMRFLDQKDVEGCAALVKKAHDLGI
                     TYFDTAIGYGDSEAIFGAAFREMKKQRRERPFYVSTKTNKATAGEIRRELETSLKRMG
                     LDYVDFYHVWCVMDKDSYLKRKLKGALQAFEQLQREGLIRHICVSSHQTGVEIEWMLQ
                     DYPFAGILLGYSVMNYAYRDQGISAAARLGRGVVIMNPLGGGIIPQHPQRFDFVRTRE
                     EETVVQGALRFLLNDARITTALVGFSSEAQLEEAVAAAEGFAGLNAEAVEGIRSHLST
                     SFDELCTGCGYCDHCPVGVPVPKMMDSYNQFMLSGRPESIAERLGWHWGIGRKSPVYD
                     LCTECGQCEEACTQKLPIRARLREIREHVQKGRK"
     gene            complement(18281..20389)
                     /locus_tag="PKM23_01965"
     CDS             complement(18281..20389)
                     /locus_tag="PKM23_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90268.1"
                     /translation="MNGSIKAWMAGWAALLALGTGMTGCSLEAPKAPSWDTTLNIPVA
                     DHTYSLHELIDREENLKAGLDSTLTFHFEEALDTIAVGDHLTLPDIHESLSFGLDAFR
                     IPNVPVMADRFLWASLTPQAAVPAGTTGVVSPFFFNYIPSSVHNSQDLLYALLVSGMA
                     RLHLYNRLTVDLENLILSLQDSATGRIVVSSPLVPRIAALDSGAVNVDMRGCAIPRQG
                     RWLLSGNSPGSKGAVVPIDGEFPFDVVVELFDFVIAAAEARIPAMTIERTDTVQLGEN
                     GGNAIAEAAFASGRMMVSLDNHSPFASPEITFTFPQIRKSAAGAPLEIPLRLQPDGLT
                     RAEIDLAGLTARLPLPPPGAHQAIEVAVRAVTDDMRSSFKEVGEATRLTMRVDMENLR
                     LDHFNGRLATREVRIDSSLRDLDISGEWGDLDGVTLREARLLVEIFSTLNLPVRLKGA
                     LYGFSGGRAGTPFAVDISVPAASGGPLLYRAPLYTVNNSGIVEFINQHPEQIAVAGSA
                     WIGDSGAAGGVRRQDRISARFSLELPFILSWEERWLDGDLHLLEISPPGAEGDAVEER
                     DDHLVLAGDATEHLRSAELELALENRLPAAGEVTFYFATDSSRLFTSPDLVVGPVTLE
                     AAAVDASGRAAAPALEKSTLTLSAEELRLFQNGGGVVKNLYFSHRVRIAGSGSRSLRL
                     HLADYLRVQALLRLVVRIGG"
     gene            complement(20468..21343)
                     /locus_tag="PKM23_01970"
     CDS             complement(20468..21343)
                     /locus_tag="PKM23_01970"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNY90269.1"
                     /translation="MKIGFIGLGIMGEPMALNLLKAGFPLTVYNRTAGRMARLVEAGA
                     AAAGSPREVAAASDTVITIVTDSPDVEEVVLGAEGILAGLRPGSTVIDMSTISPAVTR
                     RLAAEIEKAGSEMLDAPVSGGDIGARAGTLAIMVGGREEVFTRCLPVFQAMGKSATWV
                     GGHGMGQTVKLCNQILGAVTNLAVCEAVEFARKAGVDPLIMIEAVKGGAAGSWQLANL
                     GPKMAAGDFAPGFMIDLQQKDLRLALEAAREMQLPAPALALVNQLFAGCQAHGEGREG
                     TQALVKSLRRLGGNI"
     gene            complement(21378..21812)
                     /locus_tag="PKM23_01975"
     CDS             complement(21378..21812)
                     /locus_tag="PKM23_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNY90270.1"
                     /translation="MNRWLTLVLMLGLLALGCSKSGPDPEIESVVRAYHAAFDARDLT
                     ALRAYCAKGMFWYTLNGKELNASQIADFFTPMLARWESLQTSLSGMEIRREGGLAVAR
                     YRSDLHITTNGKGSTMQNLYTTVLVREEGDWKVWQHHMTTSY"
     gene            complement(21805..22674)
                     /locus_tag="PKM23_01980"
     CDS             complement(21805..22674)
                     /locus_tag="PKM23_01980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNY90271.1"
                     /translation="MAEIKVSVILTTCDRAALVSEALASVLEQSRPPAEIIVVDDGSA
                     DETEERLRPLAGGVHYLRNPQPLGVSAARNRGIRAAAGNWLAFLDSDDLWKPAKLERQ
                     LQALREHPGYRICYTDEEWRRDGRWMNQGARHRKRSGWIYTDCLPLCIISPSSVLIER
                     GLLEQTGLFDEALPACEDYDLWLRIASRVPVLFVPERLIVKRAGEWPQLSRQHSLDRY
                     RIIALAGIAASGGLGAADLAATTAMLAEKCRIYALGCRKHGRTEEAEWAENMASGRAG
                     LTQSPIMEETNNE"
     gene            complement(22699..>22959)
                     /locus_tag="PKM23_01985"
     CDS             complement(22699..>22959)
                     /locus_tag="PKM23_01985"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNY90272.1"
                     /translation="RHRGGAFGEALFRQVGSWALMAFVFGFLFPGVNNWAHAGGFAGG
                     YLSALLLGYQEIRGERTGHRIAAIVLAFITLAGFVLSLTRAA"
BASE COUNT         4446 a         6975 c         7450 g         4088 t
ORIGIN      
        1 gctcgttcgt aatcctcact gcggacagcc cgtcgcagtt cgcgcgccaa ttggtcgcgg
       61 tcgctgatgg gccgtttgct gcggatctcc acagcccact cctgcagcag ctccagctcc
      121 accctttcat cccccgcctt ttcaccgtgc atgcggtaaa aacgcctcag cctgctcctc
      181 cccttccgaa tctgctccag agccttgtca taatcctgaa actccatgca ctgtgagacg
      241 gcggcgcggg tgttcatcat gatgacatag ggacggtact gctcaaagga ccagatgatg
      301 gtctcgttgc tggaatggct gccgacaaaa tcaaaaagct gcaaattacg tttggtatct
      361 cgaaggacgc ggccgaaatc ccccagacgc atcagggcga cgtagcgatg gtagagctgg
      421 atggcctcct gctggagcag ccagcagtcg aagggggtca ggtttaagct cctggcttcc
      481 tccttttcgg ccagccggga cttgtggagg taatagctgt agtacgattc gaaatggtga
      541 ggacggcggc cgtccggacg tccgtcaagc tccatctgca ggaggccgag atcgagacgc
      601 agctggagct tttgccggcc atcctccccc tggatgaggc gcacattcat atcgttggcc
      661 gagtagtgcc acttgcgcag aatgggtccg atgtcatccg ccataaaaac ctcccgacag
      721 gagccgtcac tcgccggacc ccgggcgcag ggttaccgga aataggcgac cgcgttgcgg
      781 aagatagcca cgccgtcgcc ctcccgggcg gccccatccc gcatccagcg cggatggttg
      841 gtgggatcga tgtgccgttc cgggtggggc atgagtccca ggattctgcc cgtgggatca
      901 cagatgccgg cgatgtcccg aactgaaccg ttcgggttgg ccggataggc ggcggagccg
      961 ccatccgcag cgcaataacg aaaaacaatc tggtgattgg cttccagccg ttccagaacg
     1021 gccgggtagc tcaccgcaaa ttttccctcc gcatgggcga ccgggaaata gacctgttcc
     1081 ggcatcgggg tggtgaatac agaggggctc tcgtgcacct taaggtagac ccagcggtct
     1141 tcgaatttac ccgaatcatt gtggacgagg gtgactttct gtccggcgtc cagatccagc
     1201 tccggtaaaa gtcccgcttt tactaacacc tgaaagccgt tgcaaattcc gatgatcaac
     1261 ttgccggact ggtgaaagcc ggcaagctct tgggacagtt tgtagcggag ttcgttggca
     1321 aggattttgc ccgccgatat gtcgtccccg taggtaaagc ccccggggat ggcgagaatc
     1381 tgatagtccg ccagccgcac ctcttgccgc agcaggcggt tgatatggat catctctgca
     1441 cgggcgccgg catattcgaa ggcatggtgc gtctccatat cgcagttgga gccggcggcg
     1501 cgcaggacca gtactcttgg tttcgtcatt ggatcaccac ctcatggttt cttgccagga
     1561 gttcttgagc aggtcgatgg cttcgtccac caccagctcg ccgatgaggc cgcggatgcg
     1621 gaggcgcggc ccggaggtga cctcgccgat gcgggcgcag ggcagctgca tgaagcgggc
     1681 gcggaactcc tcctctttgc cgggaatgac ctcgacgatg aagcggctgt tggattcgga
     1741 gaagagggcc cagtcgctgc ggagcagatc ggcgcttctg ggcaggtcgc agagggtcac
     1801 ctcggcgccc aggcccccgg cgaaggccat ctcggccagg gccgcgccca ggccgccctc
     1861 ggagcagtcg tggcaggcgc gcaccagtcc cgcagcgatg gcgtcggcga gggcgatgaa
     1921 gagggccctg gcgcgggcgg gatcgaccct gggcacggcg ctgctgctgt ggccgatcgc
     1981 ctcgtaatag tgcgatccgc ccaattcctt gccggtttcg ccgaggatat agagggcgtt
     2041 gcccggacgc ttgaggtcca tcgaaaccgc cgtgcggcag tcctccatca ccgcgatggc
     2101 ggagatgagc agggttggcg gaatggcgat gctctcgccg ctctcggtct tgtattcgtt
     2161 attgaggctg tccttgcccg agatgaaggg agtgcccagc accagagcca tgtcatagca
     2221 ggcctgggcg gcgcgcagga ggccgccgag gcggtccggc ttgtcggtgt tgccccagca
     2281 gaaattgtcg agcagggcac agcgttcgag gcgtccgcct acggcgatga tgttgcgcag
     2341 ggcctcatcg atggcggagg ccgccatcca gtagggatcg atgaggccgt atttcggatt
     2401 gaggccgttg gcgaggatga ccgcgcgatg gctttccaga cggggcctgg tgatgcagcc
     2461 atcgccgggg ccgtcgttga ccacccccac caggggcttc aggacgctgc cgccctgaac
     2521 ctcgtgatca tactggcgga tcacccactc cttgctgcag acgttggggg cggcgagaat
     2581 gcggtggagg atctccccca ggctggcggg ctgggccccc tcgctctcgg cgatggccgg
     2641 ggggtcccag cgggccttgc ggacaatgcg cggctggcca tcatggagaa aggccatctc
     2701 cagctcgccc accacctggc cgcgatagcg caggcggatt ttgtggtcgg tggtggtgcg
     2761 gccgatgaca gtcgcctcta catcctcggc ggcgaagacg gcgagggcct gttcgagctt
     2821 gtccggcgcg acaaaaatca ccatgcgctc ctgggcctcg gagatccaga tctccatata
     2881 ggtgaggccg tgatatttga gggggatgaa ttcgaggtcg acctcggcgc cggtggtttt
     2941 ggcgagttca cccaccgccg aggagaggcc gccggcccca cagtcggtga tggcgcggta
     3001 gagtcccagg tcgcgagcct gcatcagggt atcgaccagc tttttttcaa cgatggggtt
     3061 gccgatctgg accgctccgc tccaggtgcc ctcgctctcg ctgtggagct cgcccgaaga
     3121 ggcggtggcg ccatggatgc cgtcccggcc ggtgcgccct cccaccacga caatatagtc
     3181 gcccgggacc acctgttttt cgcatttgtc acgagggatg aggccgacat tgccgcagta
     3241 gaccagggga ttgccaagat agcgttcatc gaaatagagc gccccgttgg aggtcgggat
     3301 gcccatgcga ttgccatagt cgcgcactcc ggcgaccacg ccctgcatca cccgcttggg
     3361 atggaggacg cctcggggca gctggtcatg gggcagatcg accgggccga agcaaaaaat
     3421 atcggtattg atgatcggct tggccccgag gcccgtaccc atcggatcgc ggatcacccc
     3481 gccgatgccg gtgttggccc cgccgtaggg ctccatcgcc gaaggatgat tgtgggtctc
     3541 aaccttgaag cagacattat aatcgtcatc gaactcaatg ataccggcat tgtccttgaa
     3601 gaccgagacg caccagggca aattcagctc gcgggtgacg cggaagatgg tctccttgag
     3661 ggggttgtcg ataacacgcc cctcgaattc gaccaggccg gtgagggttt tatggctgca
     3721 gtgttccgac caggtctggg cgatcatctc gagctcgaca tcggttggct cgcgctgcag
     3781 ggatcggaaa taggcctgga tggtctccat ctcggtcagg ttaagaaaga ggtcgcgctc
     3841 gcgactgatc ttcatcagtt cctcgccgga ggcctgcagc agggagacat ggatgagctc
     3901 gaaggggggt tcctcggcgg gaaaaaagag gcgctcgccg ggctggtgga ggtgctcgac
     3961 caccttgttc atcaacaact tctgggcgat gagttccttt tccgcagcgc tgagggagcc
     4021 gcacagttcg taccgccggg ccgtccgcac cgcctcgacg gcgcgcagac cgagatcaat
     4081 ggcccccttg cgcgccgatt cggccaccat gtccgtgacg ccgcggttga gggccacttc
     4141 ggcggaccag aattcacctc gactgcgctg cagcagggcg ttgatgatcc agtcgtcggt
     4201 gacagcatcg gccagcagcc tgacggcgat gagttcctgc tcggcagggg tgagctcgcc
     4261 gagaaaataa tagacccgga aggcatggac ggccgttaat gccctgaccc caagatcgcg
     4321 cgccccggcg aggatggcct tcccttcggc atcatgatac tgttcttttt gggcaagttc
     4381 tattcgggtg atcacgtcag ctcctcagtt gaagaattat tctagtttac atacttttgc
     4441 agggaaatgc aatcgaaaaa gcggccgctt ttcagcaggg ccggcggctg cctcctcgcc
     4501 tcggccggcc cgcaagggcg gaactagcct ccggcgcgga aaaccacacc aaaaagcttg
     4561 actttgacgc cgaatattaa taaattttgt cacatgtcac aaaattatgt ctgccttgag
     4621 cggctgatag tcggggagat tgctggacag cacagagtta ggcatcggac caatgtagga
     4681 gcggctaatc ttggattgtt acaggcccga gttaccccca tacccctgca ccgcccataa
     4741 caggccagtt cttccggcgc gttttttggc cgccgccaga agagctatca ggcggcatcg
     4801 cgcctcgcca gtcggtttca tcgcactgct gctgattttg cgcgccgcca ccccactcgg
     4861 cctccaggcc ctgcccagcg attccacctc ggcaccggca ggcgccatcc aagtcttcaa
     4921 ccccgatcgc gcacccgcgc gctcagccgc tgatatcgcc gctgaaatca tcgccgccga
     4981 gaccgctatc tacgcactgc ggacagcctc ccccatcacc tttaaatcgg acagcaccgc
     5041 ccccgaaatc atccccgagc tggccatcca gcgcctccgc ctcgaactcg gccagaaaat
     5101 tcaggcgatc tcgggaccgg actccacttt ttatcaaccc ttcgccggcc tccgctcagg
     5161 cgcccacgac gaatcctccg ccggcccggt gcaccgcatg caggaggccc tgctgcgctg
     5221 gaacaacaaa ctccccctca agaccaacgg ccactacgac gaagccacca tcaaatccct
     5281 caccctcttc aagctggtct atgacctcgg ccatgacggc tccttcgtcg atcaggccac
     5341 cgcgggctat ttgctggcca ttgaggaggg caacgaccag gaacttgccg aaccccaggg
     5401 gttcaccggc cgcctggtct atcacgccgc ccaattcctc ggcctgcgct accgcctcgg
     5461 cggtgacggc aaaaagacga ccgactgcgg catgttgact cgcatggcca tgatcagcgc
     5521 aggactggtc gacgaggtct ttaaccgcgt cgccgccacc cagtacaaat acgctgaaga
     5581 gggcaagatg gggctgaccc tggtcgacaa aaatgaggaa cccgcccccg gcgatctggt
     5641 cttcttcaaa tggaataccc ggcgcgcccg ccaccgctac aagggcatca cccatgtcgg
     5701 tatttttctc ggcaaggtcg gcgagagcct ctatgtgctc gaggccgcct ccgcccgcaa
     5761 gacccgcaag gtcaccatcc tcgaccgttc caactcgatc cgcaagatcg ccggctacgc
     5821 ccgcttcacc gccccgcctg aggatgggcc gatcgagccc gccctggaaa tgaccgacta
     5881 gcccttcaat ccgggtctac aacagcaaag cctcccctgc ccatcggcag cggaggcttt
     5941 tttatgccgg cggcgcgccc ggcctcacct gcatggccgc gcatccgcag taaagcacga
     6001 acgatcagcg tatcagcgtc agccggcccg tgcgcacctc ccctccttca acacgcagct
     6061 ggtagagata gaccccgctg gcagcactca tgccactgtg gtccctgccc tcccagcgca
     6121 gccggtgaag cccggccggc tgcctcccct ccgccagggt gatgatccgc ctgccgagca
     6181 gatcgaagat gtggaattcg acatagccct cccggctcag ctcgtattcg aaggtcgtcg
     6241 aggcattgaa gggattgggc cagttctgcc gcagcacgaa agcggcgggg ccctcccgcc
     6301 cggcggccgg cgcctccacg cccgaggccg tgctgtcagg catgaaaatg gtataggcga
     6361 cgcccccctt gtcgaaccag gcgtgcgaaa ggtcgttctt gtcatagata tagccgtagc
     6421 cggtgttggt gcgggccggg tcatggacga tcggatcccc attcgccgta aagccggcca
     6481 gcatcaccag gtggccgctg taaagggggg cgccgatcga catgccaatg cggcctcccc
     6541 gcgcgagcac ctcccgcgcc tcgccccagc tgcggtagcg ggtgaccgtc cccttcaccc
     6601 cgtactcgga ggcattctgc accacccgcg gccagacccc gaaaaggcca tagtaggggt
     6661 cgcgcgtatc ccgggcgaac tggaggggat caacggcgat gttgaagctg agcagaatca
     6721 tcgccaccgt ggtcggcgag cagatgctcc ccccgatctc gggatcaatg cggtactggg
     6781 cgagaaagct ggtctgaacg aagatcgcct cgggcttgtc cctgaggatg gctgcgtaat
     6841 ccagccccgt tgtggtgcgg ctgtcgctgg cgaaccacga gagcagggcg atcgaggggg
     6901 aggtcaccgc cgccgtgctg cgcctgaatt cgacggcgaa ctgccatgcg ctggcgtaat
     6961 agttcaggcg ggcgatgtcg atatcgatct ccccgcccgc aaaggtggtc ttcttgccgg
     7021 gccaaaggtt ggccttccag aaaccggcat cgagccaggg cgaccagccg ctgagatagg
     7081 gaaagcgcac cagcacccgg aagcccccgc tgtcgcccgg tgccgtgccg ttccatgagg
     7141 gcagcccctc gtcgaaaggc cagggcgcct gccagggttt gaggatgaga aaaccgctcg
     7201 tcgccccgtc ttccagcctg agactcttgc gatcggccga gaggaccacc cggtcgagac
     7261 tttcagcgtg cgcaagcagg gaatccccgg attcgatgac gaaatgctga tcgggataac
     7321 tgctctgcgc cctcaccatg gccgcccccc aggtgagcaa tatgagcatc acagccctgc
     7381 gtaccatgct agtctccaac tttgtcaacc tcctccttga acgggatcga taccggaccc
     7441 gccgctccgg gatcggcgcc tagtccggcc tcgtgcttac gaccgagctg acggatttca
     7501 taccatagaa aaaatgcggt gaccaggacc gagaggagat cagagacagg ggaaaccatc
     7561 cagatcccat ccagtccgta gaagcgggga agaatggcca gcatggggat gatgaagatc
     7621 agctggcgcg acagggcgag aaacatcgcc tgtttcggct tgccgaccgc ctgaaaatag
     7681 ctggaaccga tcacctgaaa accgatgacc ggcatcacca tgaaaaagat gcgcatggcg
     7741 tgggtaccca tggcgataag cccggcgtcg cggggattga agaggcgcat caggggagcg
     7801 ggaaagaaca tcaccaccag aaaccctgtc caggccacca ccgaggccgc cgcggcggcg
     7861 agccggaccg tcgtgcgcac gcggtcgtac ttgcgggcgc cataattgta gccgatgatg
     7921 ggctgactgc cctggttgat gccgaaaatc ggcatcagga agagcatggc gatgctgttg
     7981 aggattccca tcaccgaaat ggagagatcg ccgccgaatc gctgcagctg gttgttcagc
     8041 aggacggcat agaggctggc gacgatgtgc atggcgaagg gagccgaacc gatcgcggcg
     8101 attttcttga cgatcgccct gtccagcctg agattggccg gccggatctt gagcagactc
     8161 ccccggccga aaaaatagcc caacacccag gtcatcgagg ccatctgcga aatcaccgtg
     8221 gcgatggccg ccccacgcac acccatgtga aagacaaaaa tgaagagcgg atcgagcagg
     8281 gtgttcagtc ccgcaccgat gaacatggtc ttcatggcca tgcgcggatt gccctcaccg
     8341 cggatgaaat ggttgagacc aaacccaacc ccggcgaaga cggtgccgag aatgatgacc
     8401 tgcatgtatt ccacagcgta gggcatgctc accggactgg caccgaagag gcgcagcagg
     8461 ggacggagga aaatcaggct cgggatggtc agcagcaggg agatcgcggt aatcagaacc
     8521 agggcattgc cgagcacctt ttcggcctcc tcccggcgct gttcgccgag gcggatggag
     8581 accagggcgt tgccgcccag gccgatcagc atgccgaaag ccatctgcac ctgcatgatc
     8641 ggaaaaccca ctgtcgcgcc ggcgatgcca agggagccca ccccctgccc gataaatatc
     8701 cggtcgacga cgttgtagag ggcgttgacg accataccga cgatggcggg gacggaaaac
     8761 ctgatcagca gtgcggagac cctggcctcg ccgatttccc tggcgttgtt catgcggatg
     8821 accggtatcg tgaaatttcc aagactacag tagcggggct gcgacggatt ttcaccgtcc
     8881 tccctattga tcgcgatgga accagagtct tgaatgaact gacaaaaggt aaggaattat
     8941 tgtgaaaggc gcaggaaatt ccggtcagtc ataaaaattc caaacaaagc cccagcggat
     9001 cagccgccgg ggctggggat agcctcgcac cacctcgtag tcgatgtcga ggggattctg
     9061 cagcgcaaaa aagagggtcg catccctgat gaccacgctg agatcgagcc agggcaccgc
     9121 cgcggctgcg aggggttcgc tctcctttga ggcatcaacg taccagggca gggggccctc
     9181 gcgttcaccc cagaagaaag aacccaggcg caggcgcaca tcgaggtcct tgtgaaaaaa
     9241 gatatggcgc acccccagcc agccggcggc gcaggtcgcg gactggttca ggggggcctg
     9301 ctcctcgaag agcatctgtt tggccttgcc cgccagggtg aaccaggagg agaggcgcca
     9361 ttcaccggcg aggtcgatgc tgaagcgcag ctggccgggc tggttccggt aaagcgcagc
     9421 tgtctgcggg gcctcctgga cgagcatcag ctcctcccgg gtgcggctgg cgctgagaaa
     9481 gaggaggagc ttccaggcgg gggcgtggcc gctgaggccg aggccgagat ggtcacccga
     9541 ttcggcctgc agctcaggat cgcccagggc gaaggggccg gtctcgtagc gttccgcgag
     9601 cgaggcctct ctccggctgc gctcgtacca gagcagggat tgccagcggg ccggcgatga
     9661 ggcggtggag ctgagggcag aatccagagg tgggccggca tcgccggtcg ctgtcgagtc
     9721 cggccgcgca gaagtctgtg gagaaggccc cagatcataa cgcagctgca gctgggggag
     9781 cagggcagcg cccatcccct cttctttttc gaggcgcagc tgggtgaaag ccatcaggcg
     9841 gggcgcggga tggagaatga ggcttcccca accgctgcag aggctgcggc tgtgatctcc
     9901 ccaggcaggg ctcttgagtc cggtgctgcg gaaggtcccg ccggtcgtcc ccgacaggga
     9961 gccccgccgc cagagccagg tgctggtcag gtcaaagcga agcacatcgg tggtctcatc
    10021 ccagaggctg tggcggtagc cgtagcgctc ccggtgctgg tcggtgagct ggaccagggt
    10081 gcgccacgaa ccggcgtaat cgatctcgag gccgtggtcg tagcggctca gcttttgatg
    10141 cggctggcgc aggtcggggg ccgccggggg cgcctcgggc agggggaggt tcaggtcgga
    10201 gatattgtag agcagacggt agcgggcgcg ccagaggctg ccgaaggtgc gggtgagggc
    10261 caggttgatc ttttgcgcgt ggtatttctc cagctgcgcg gtggcgccgg catagttttt
    10321 cagcaccccg ccaccattga tccacagccg ctggctgtag cgcagcccgg cacggacgtc
    10381 gatgtcgtca tagccgttac cgccgctgcg gtaggcgacc tgggtgcgca gggtatcagc
    10441 ggcgaggtcc cgcccggtga cctcgagggt ctggcttgcg ggccatgccg gaaaaggcct
    10501 ggtgagggga tcggcgaaaa ggacctggct ggccatgctc tctacgggga tgaggttgag
    10561 gtcggagagg ccggtgagag gatcgtcaag gcgaaagccg tcatagatca cattgagccg
    10621 gttgggcggg gctgcgaaga gagcgcccaa agcaggaagg ccgacgctgc cgcggtcctg
    10681 gtaacaggcg ccgggcagaa gggcgaagag atcccccgcc gagccgtagc cggaaccaag
    10741 ccggagggtg gaatcggcag aaaagggggc cgacatagtc tgcccctgcg caccggcggc
    10801 ggtcatcagg agaacaagcc aggcggagat tctgcgcatg cgcgctccat cagcgagtga
    10861 ttataaaccg cagccgctcg agcaggagcg gaagcagcag ggcgaagatc agggtgttgc
    10921 tgatcatgtg cagcccgtaa aaagccatac catagacaaa agaggcccaa accttggcgg
    10981 cgtccccgcc ggccatgatg atggcaaagc tcagggtggt cagcacgtca tagatcaggg
    11041 tgagcaaaag cccggcgccg ccgaagagcg ccgtccgaat ccagaccgga cgctccagcc
    11101 agtgcgcgcg ctgcaccagt ccgccggcaa ggccggtgag ggccatgctg atgatctggg
    11161 tgagcagcag cgggggggcc gcggccccgt agggattgaa aagggaaaag agcagagctg
    11221 cggcggcgcc gatgagggcg ccgcggccgg gtccggtcaa aaagcccgag acaaaaaccg
    11281 cggcggtgaa catctccacg ttcggaatgg cgataaagag atagccgacc gtcacgctca
    11341 gtgcaaccag cagggcggcg agcagccagt tataatagcc ggcgcggtgc tgcaagggaa
    11401 ctccatggtt aatcgacatg caatttagag aagcgcgggg ggaatgtcaa ggagtttttg
    11461 gcccgcacgg gaagtcccgg cggacccgcc gccctctccc ctgctggagg atgttcgggc
    11521 gcaggaatgc aaggctcgat ttttttcttg ctttccagat ccggattcag tataataagg
    11581 tgtacattat tacacttttt caggatggcc atgcatttcg agaccgtcaa tgaatcggtc
    11641 gaggtgatca gctacttcga cggccggaag atgcggccga tacgcttccg ctggcgcagc
    11701 cggccctatc atatcacccg catcaacgcc atctggagtg agctgcaggg gcgcgactgc
    11761 ctctaccatt tccacgtcac cacccgggag tcgggctcct tcgagctcat ttatcagaac
    11821 agcggcctca cctggcgcct cggccgggtg ggtgtggagg agtaaaaacg gaacggcccc
    11881 gccgccggaa gcgggcggca gggccgggga tttatgcaag ggaaatcggt ctacaggtcc
    11941 tcgatctttt taaagcgcgg cacgaggaag tgcatcaccc cccaggccag caggtaggcc
    12001 agggcgcaga aagcgaaaac catggtatag gcgaaggtca tcttgtcctg cacgatcggg
    12061 acctgcagtg cccgcaggga ctcgaaagct gcggggtcga tggcctgaag cttggccatc
    12121 acctcgtggg gcaggctgcc cagctccacc cggtcaagat tgatgatgtc gccatgcttg
    12181 ttgaccaggg agagggactt gatcttgtcg agaaagccgc cgaggttggc cgcctgggcc
    12241 tccacaaagg atttggcgat gccggcgctg cggtagccgt cgaagagcca gccggcgatt
    12301 ttgttgatga cgatgccgcc gatgcccccg gccatgccgc cgatgccggt gacgctggcg
    12361 accgctttct tgggaaacat gtccgagact gtggtgaaga tgttggccga ccaggcctga
    12421 tgggcggcgg tgccgatgcc gatgatcagg accggcagcc agtagctgat gccgcccagg
    12481 ggctgggcca gaagcaccaa gaggggtaca agtgcaatga ggagcatggc cttcatgcgc
    12541 gcggagtaaa cctcctgacc cttgttgata aaatacatcg gcagggcgcc gccggcgatg
    12601 ctgccgatgg tcgacatcga atagagcacg gcaaggggaa aggcgaccgt agtgccggtc
    12661 atgccgtact gcgccttgag ataggcgggc agccagaaga ggaagaacca ccagaccccg
    12721 tcggtcatga acttgccgaa gctgaaggcc caggtctgtc tgtatttgag cagctggaac
    12781 caggagatct tttccttgac ggcactgccg ctctgcgtcg gggcggaatc atcggcatcg
    12841 ctgtggatga aggccagctc ggccggcttg acccgcttgc tcaggcgcgg ggtctcatag
    12901 agccagaacc agaagatcag ccagatcagg ccgatggcgc cgacgaccag gaaggcggcc
    12961 tgccagccca gcttgttggc gagccagggg acggtcagcg gcgccaggat agccccgacg
    13021 ttggcccctg agttgaagag gccggtggcc agggcgcgct ccttcttcgg gaaccactcc
    13081 gcgatggtct tgatggcggc gggaaagttg cccgattcgc cgaagccgag aaccccgcgc
    13141 gcgaacatga atccgccggt tccccgggcg agggcatgga tcatggcgcc gagcgaccag
    13201 atcagcaggg agagggcata gccgagtttg gtgccaagcc agtcgatcaa gcgaccggcg
    13261 aagagcatcg atatggcgta gacgaactga aagaccgcga cgatgttggc atagtcggag
    13321 tcgctccagt tgaactccac ctcgagccgg tccttgagga ggctgagcac ctggcggtca
    13381 agatagttga cggtggtggc gaaaaagacc agagcgcata tggtccatct gtacttgccg
    13441 atcttttcac tcagggtcat ttcgattcct ttccggttag ggcggcggga tcaggcgatc
    13501 accagggtca tgatcgaatg gggcaggctc tccacggagg cggccttgcc cttcagccag
    13561 agtttgcagg cgaatttctc atccgacagg ttgaggacga ccacggctat ccggccgtcg
    13621 ggattgacaa aggcggtggt catcagcttg tcacggttgg aggaggcgat gatccgcctg
    13681 gccccgggcc ggatgaactt gctgaagtgg ccaagatagt aaaaagagtt catgtaatag
    13741 agatcgcctt cagcggtctt gtgcaccggg gcgaagcaga aattgccgac atggttgggg
    13801 ccgccggtct catcgaggag cacattccag tcggtccagc ccaccgcacc gttgttgaaa
    13861 tcgttgacca gggatttgcc gtagttctcc ccccagtgcc attgcccgag gttttccgtc
    13921 ttgtagggaa agttgcagcc ctcggtgaag atgagattgg cctccggaaa ggcctcctgc
    13981 acccgtttga cgttttcaaa ggcgtcgttc atgtaccagt ggaatcccac tccccagacg
    14041 tagcgggccg cttcgggatc gttgagcact gtactggcgc gctggtagag gagatcacgg
    14101 ttgtggtccc agatgatcag ttttttgccg cccatgcccg ccctggccag ggccggtccg
    14161 agatgattct tgacaaagtc gcgctcctcc tcatcggtgt agatgcagga ctcccaggtc
    14221 tgggtggcca tgggctcgtt ctgcaccgag agaccccaca tcggcaggcc ctcctgctcg
    14281 taggactgga cgaactttat atagcagccg gcccaggcct caaaacactc gggtttgagt
    14341 ttgccgccgc gcagcatgtt gttgttggtt ttcatccagg ccggcgggct ccaggggctg
    14401 gcgtagaggg tgagggcacc cccggccgcg gccgtggcct ttttgatgaa gggaagcttg
    14461 aactgtcgat catggtcgat gttgaaactt ttcagctcga ggtctccggg cttgacatag
    14521 tcgtagctgc cgcttgaaaa atcacagctc tggatgttgg tgcgcgccag ggagtaaccg
    14581 attcccttgt gcggatcgaa gtaggccgtg agcagctcct gctgcttctc ctcggagagg
    14641 ctgaaaaaag tctcggccga ggcgtcggtc agggccccgc cgatgccgac cagggtctgg
    14701 aaggtatggg ctggatcgat aaagatgcag ggctgggtct ccaggggctg gccgaagggt
    14761 tcgaaggcgg ccgcgccgag gtcggcgagc cggttgccgt tcgcctgcgc ggtctgatag
    14821 atcctcacgg cggcggggtc cgcacccccg gcgggggcag ctttaacctg ctcgttcttc
    14881 ctggtgacgc aggccgcgga cagcatgagg gtcacggcca gggcgatcat catcttgttc
    14941 acatctcctc ctttataaat agcccatcca ttccggtgcc gcaggcggga caggaacccg
    15001 cctgcaggtt attggcgacg atttgaaaac cgtagcgtcc gatcagcagc gcgccgcagc
    15061 ggtggcagta gctgttctcg ccgatctcgc cgggtacatt gccggtatag acatagcgca
    15121 gccccgccgt gagtccgatc ttgcgggcgc gctgcaatac ggcaaggggg gtcggggaga
    15181 cctcggtgaa acgatactgt ggataaaaac ggctgatgtg ccagggggtc tccgctccca
    15241 gctctttgca gatgaaacgg gcgacctcgc gcagctcgtc ctcgccatcg acctggcccg
    15301 ggacgatcag ggtggtcacc tcgacccaga tgccaagttc tttcatcagc ctgagggtat
    15361 ccagaaccgg ctgcagcgtt ccgccgatga cgctgcggta gaactcgtca ttccagccct
    15421 tgaggtcgac attggcggcg gtgaggaacg gggcgagctc gcgcaggggc gcctcgctga
    15481 tatagccgtt ggtgacccag accgtatcga tgccctgctc ccgggcgagc cggcagattt
    15541 cgagagcata ttcataaaaa accgtcggct cggtataggt gcaggaaatg gtgcggcagt
    15601 catcggccag ggcctggtcg acgagctgac ggggcgtgac cgggcggccg ggcaccgtgc
    15661 cgctgccacc cttgatctgc gagatctcgt ggttctggca gaagcggcag tgaaaattgc
    15721 agccggcggc ggcgaccgaa agggaacggc tgccgggata gagatggtag aggggttttt
    15781 tctcgatcgg atccacatgc acggcgaccg ccttgccata attcaggctg gtcagctcgc
    15841 ccccgtcatt cctgcgcacg ccgcaaaggc cgctcctgcc gggtgcgagg atgcaggcgt
    15901 gggggcagag caggcactgc acctggtctc ccggcagctt gcaggcatga agggctggat
    15961 gcatgggcgc tcaccttctg gcgcgcatga gcgtgtggat tttctcagcc agctgccggt
    16021 gtttggcagc ggccttttcc tcgggaagcg gcagaaagca ggcgacgacg tagcgcacct
    16081 ggccgtcatc gacgatgccg gcgtcatggc tccagctccc gtaccatccg gtcttgtggt
    16141 aaaacatcgc cccgggcggg agcccgacgg ccaatttggc gttgtcgagc tggcggccga
    16201 gcaggcacat catctgcatg ctcacccagg gattcaccag acggccggtg gcgatccggc
    16261 agaggaaatc ggccgcatgg agggcgcagg tctcggtgcc gcgaacggcc tcatagccgg
    16321 ggtcttcgaa cctgcgcttg agaaacttgc gggtcacctc gctgccctgc cagccatagg
    16381 tgtgcatcag ggagtcgatg ctgcggcgag tggcgaggtc gattaggcag ttggccgccg
    16441 aattgtcgct gcgggtgatc atcagatcga gaaggtagtt gacggtgacg gtatccctcg
    16501 cctgcaacag ggggcgtgga tcgctggtcc actccttgga gcggtcgaca gcattgtggc
    16561 cggcgatgat atggctctgt tccaggctga gccggccctg gctgacctga tggaggattt
    16621 ccgcagccac atacatcttg tagaccgagg cgggatagat aaaattgtcc atctgcaccc
    16681 cgcccaggcg gggctttttg ccggtgaggt cgatgacggc gagggagatc tgttcgaaac
    16741 cgtcctctcc ggtatcgaag gagccctcca gagccagctc ggagacaatc ccttcgagtc
    16801 cggctgcgag gccggcatcc tgctttaaat aggtgggtat gagcggggtc tgcgcaccgc
    16861 ccaggcctgt catcagcagg ccgagcagga gcacccgggg cgttttcata gccatgctcg
    16921 catcaagagg cccaaactta cggagaggtg cggctgcgct gcaagcgcaa tcaagctgaa
    16981 tttactaatc gctttccaca gtcgcaacat ctttttgccc gcccgccgga ctgcgcctcc
    17041 ggcaacggac agctgtcggt ctgtgggata aaaaaatact tgttcgccag cgaaaagttg
    17101 gataagtttg ttaataaaag cggccgcgac acggccggaa ttcgacctcc aaccacggaa
    17161 aacccgatga tctaccaccc ttatggcacc accggtctcc agctctccgc catcggcttc
    17221 ggcggcatgc gtttccttga tcagaaagat gtcgagggct gtgcagccct ggttaaaaag
    17281 gcccatgatc tgggcatcac ctatttcgat accgccatcg gctacggcga ctccgaggcg
    17341 atcttcggcg ccgccttcag ggagatgaaa aagcagcgcc gggagcggcc cttctacgtc
    17401 tcgaccaaaa ccaacaaggc cacggccggc gagatccgcc gcgaactcga gacctcactg
    17461 aagcgcatgg ggctggatta tgtcgatttt taccacgtct ggtgcgtgat ggacaaggat
    17521 agctacctga agcgcaagct gaagggggct ctgcaggcct ttgagcagct gcagagggag
    17581 gggctgatca ggcacatctg cgtctccagc caccagaccg gggtggagat cgaatggatg
    17641 ctccaggact atcccttcgc gggaattctg ctcggctact cggtgatgaa ttacgcctat
    17701 cgcgaccagg gcatcagcgc tgcggccagg ctggggcgcg gcgtggtgat catgaatccc
    17761 ctgggcgggg gcatcatccc acagcatccg caacgctttg attttgtccg cacccgtgag
    17821 gaggagacgg tagtccaggg cgccctgcgt tttctgctca acgatgcgcg catcaccacc
    17881 gccctggtcg gcttcagcag cgaggcccag cttgaggagg cggtcgcggc ggccgagggc
    17941 tttgccgggc tgaacgcgga ggcggtcgag gggatccgca gccatctcag cacctccttc
    18001 gacgaactct gcaccggctg cggctattgc gaccactgcc cggtcggggt gccggtcccc
    18061 aagatgatgg acagctacaa ccagttcatg ctctccggaa ggcccgagag catcgccgag
    18121 cgcctcggct ggcattgggg gatcggacgc aagagcccgg tctatgatct ctgtacggaa
    18181 tgcggccagt gcgaggaggc gtgtacgcag aaactgccaa tccgcgcgcg gctgcgcgaa
    18241 atcagggagc atgtgcaaaa ggggagaaaa taggccgttt ctatccgccg atgcggacca
    18301 ccagcctgag cagggcctgc acgcgcaggt aatcggcgag atgcaggcgg aggctgcggc
    18361 tgccgctgcc ggcaatccgc acccgatggc tgaaatagag gtttttgaca accccgcccc
    18421 cgttctgaaa gaggcgcagt tcctctgcgg agagggtgag ggtgcttttc tccagtgccg
    18481 gcgccgctgc cctgccggag gcatcgaccg cggccgcctc gagggtgacc gggccgacca
    18541 caagatcggg gcttgtgaag aggcggctcg aatcggtggc aaaataaaag gtcacctctc
    18601 cggcggcggg caggcggttc tcgagggcga gttccagctc agcagagcgc agatgctctg
    18661 tggcatcccc ggcgagcacg agatggtcgt ccctctcctc caccgcatcc ccttccgcgc
    18721 ccggtggtga gatctccagg aggtggagat caccgtcgag ccagcgctct tcccagctca
    18781 gaataaaagg cagttccaga ctgaaacgcg ccgatatccg gtcctggcga cgcacgccgc
    18841 cggcggcgcc gctgtcgcct atccaggcgc tccccgccac ggcgatctgc tccggatgct
    18901 ggttgatgaa ctcgacgatt ccgctgttgt tgaccgtata gagcggcgcc cggtagagca
    18961 ggggaccgcc cgaggcggcg ggcactgaaa tatcgacagc aaagggggtg ccggcgcgtc
    19021 cgccgctgaa gccgtacaga gcgcccttca aacggaccgg caggttgagg gtggaaaaga
    19081 tctcgaccag cagcctcgcc tcccgcaggg tcaccccgtc gagatcgccc cactctcccg
    19141 agatatcgag gtcgcgcagg gagctgtcga tgcgcacctc gcgggttgcg aggcggccgt
    19201 tgaaatggtc gaggcgaaga ttctccatgt cgacccgcat ggtcagccgg gtcgcttcgc
    19261 cgacctcctt gaaggaactg cgcatatcgt cggtcactgc acggacggcc acctcgatgg
    19321 cctggtgcgc gcccggcggt ggcaggggca gtcttgcggt caggcccgcc agatcgattt
    19381 ccgcgcgggt gaggccgtcg ggctgaagcc gaagaggaat ctcgagcggg gcaccggcgg
    19441 cgcttttcct gatctgggga aaggtgaaag tgatctccgg gctggcgaag ggggaatggt
    19501 tgtccaggct caccatcatg cggccgcttg caaaggccgc ctcggcgatg gcattgccgc
    19561 cgttctcccc gagctgcacc gtatctgtgc gttcgatggt catagcgggg atgcgcgcct
    19621 cggcggcagc gatgacgaaa tcaaacagct ccaccaccac atcaaaggga aactcgccgt
    19681 cgatgggaac caccgccccc ttgctgccgg gcgaattgcc gctcagcagc cagcgcccct
    19741 gccgtggaat ggcgcatccg cgcatgtcaa cgttcaccgc cccggaatca agcgcagcga
    19801 tgcgggggac caggggggag gagaccacga tcctgccggt ggcgctgtcc tgcaaggaaa
    19861 ggatgagatt ctccagatcg acggtcaggc ggttatagag atgaaggcgg gccattccgc
    19921 tgaccaggag ggcgtagagg agatcctggc tgttgtggac ggagctgggg atgtagttga
    19981 aaaagaaggg cgagaccacc ccggtcgtgc cggcgggcac cgccgcctgt ggtgtcagac
    20041 tggcccagag gaagcggtcg gccatgaccg gcacattggg tatccggaag gcgtcgaggc
    20101 cgaagctgag gctctcgtga atgtcgggca gggtgagatg atcgccgacc gcgatggtgt
    20161 cgagcgcctc ctcaaagtga aaggtgaggg tgctgtcgag gccggccttg agattttcct
    20221 ctctatcgat gagttcgtgc aggctgtagg tgtgatcagc caccgggata ttgagggtgg
    20281 tatcccagct cggtgccttg ggcgcttcca gtgaacaacc ggtcatgccc gtgcccagcg
    20341 ccagcagagc cgcccatccc gccatccatg ccttgatcga tccgttcatc catccacctg
    20401 catcaacacc ctttacagag tacatacccg catccggcag cggatgcgga gcgactttcc
    20461 ggaggcttca aatgttgccg ccgagccggc gcagagactt gaccagcgcc tgggtcccct
    20521 cgcgcccttc accatgggcc tggcagccgg cgaagagctg gttgaccaga gcgagggcgg
    20581 gggccggcag ctgcatctcg cgcgccgcct cgagggccaa ccgcaaatcc ttctgctgca
    20641 ggtcgatcat gaatcccggc gcaaagtcgc ccgccgccat tttggggccg aggttggcga
    20701 gctgccatga accggcggcg ccgcctttga ccgcctcgat catgatcagg ggatcgacac
    20761 cggccttgcg cgcgaactcc accgcctcgc agaccgccag attggtgacc gcgcccagga
    20821 tctggttgca gagtttgacg gtctgaccca tgccatggcc gccgacccag gtggcgcttt
    20881 tacccatcgc ctgaaatacc ggcaagcagc gcgtaaagac ctcctccctg ccgcccacca
    20941 tgatcgcgag ggtgccggcc ctggcaccga tatctccgcc gctgaccggg gcatcgagca
    21001 tctcactccc tgccttttcg atctctgcgg ccagacgccg ggtcaccgcc ggcgagatcg
    21061 tactcatgtc gattaccgta ctgccaggac gcagccccgc gaggatcccc tccgcaccga
    21121 ggacgacctc ctcgacatcg ggggaatcgg tgacaatggt gatgacagta tcactcgcgg
    21181 ccgccacttc gcgcggggaa ccggccgcag cagccccggc ctccaccagc cgggccatgc
    21241 ggccggcggt gcgattgtaa acggtgagag ggaatcccgc cttgagcaga ttgagagcca
    21301 tgggttcgcc catgatgccc agtccgatga atccgatctt catcctgcac tcctttgccc
    21361 tatttgtaac tgcaggttca atagctcgtc gtcatgtggt gctgccaaac cttccagtcc
    21421 ccctcctccc gcaccagcac agtggtgtag aggttctgca tggtcgaacc ctttccgttg
    21481 gtggtgatgt ggagatcgct ccggtagcgc gccacggcca ggcccccttc gcggcggatc
    21541 tccatgccgc tcaggctggt ttgcaggctc tcccagcgcg ccagcatggg ggtgaagaaa
    21601 tcggcgatct ggctggcgtt cagttccttg ccgttcaggg tgtaccagaa catccccttc
    21661 gcgcagtatg cccgcagggc agtgaggtcc cgggcgtcga aagcggcatg ataggcgcgc
    21721 accaccgact cgatctccgg gtcgggtccg ctcttgctgc agccgagggc gaggagcccg
    21781 agcatcagca ccagggtaag ccatctattc attgttggtc tcctccatga tgggcgattg
    21841 tgtcaggccg gcccggcctg aggccatatt ctccgcccat tcggcctcct ccgtccggcc
    21901 gtgcttgcgg cagcccaggg catagatccg gcatttttcg gcgagcatgg ccgtggtcgc
    21961 cgcgagatcg gccgccccca gcccgccgct ggcggcgatg ccggccagcg caatgatgcg
    22021 gtagcggtcg aggctgtgct gccgggaaag ctgaggccac tccccggccc gcttgacgat
    22081 gagccgctcg ggcacaaaga gaacggggac ccggctggcg atgcgcagcc agaggtcata
    22141 gtcttcacag gcgggaaggg cttcgtcgaa aagcccggtc tgctccagaa gcccgcgttc
    22201 gatcagcacc gatgaggggc tgatgatgca gagcggaagg cagtcggtgt aaatccagcc
    22261 ggaacgcttg cggtggcgag cgccctgatt catccagcgg ccgtcacgac gccactcttc
    22321 atcggtgtaa cagattcggt aaccgggatg ctcgcgcagg gcctgcagct ggcgttcgag
    22381 cttggcgggc ttccagaggt cgtccgaatc gagaaaggcc agccagttcc cggccgcggc
    22441 cctgatgccg cggttgcgcg ccgcggaaac gcccagcggc tgcggattgc ggagatagtg
    22501 cacccctccg gccagggggc ggagccgttc ttcggtctca tccgcggagc cgtcatcgac
    22561 gacgatgatc tcagccgggg gacggctctg ctcgagcacc gaggcgaggg cctcgctgac
    22621 gagggcggcg cggtcgcacg tggtcaaaat gaccgagact ttgatctccg ccatgggtcg
    22681 ctccacacgg gaaaccgctc aggccgcccg ggtcagggag aggacgaaac cggccagggt
    22741 gatgaaagcc agaacgatgg cggcgatgcg gtggccggta cgctcgccgc gaatctcctg
    22801 gtagccgagg agaagagcgg agagatatcc gccggcgaac cccccggcat gagcccagtt
    22861 gttcaccccg ggaaagagaa agccgaagac gaaggccatg agggcccagc tgcccacctg
    22921 gcggaaaaga gcctcgccga aggcgccgcc gcgatggcg
//
LOCUS       DAOPWU010000016        22892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_2247_length_22892_cov_5.280597, whole genome shotgun sequence.
ACCESSION   DAOPWU010000016 DAOPWU010000000
VERSION     DAOPWU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22892
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2247_length_22892_cov_5.280597"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2217
                     /locus_tag="PKM23_01990"
     CDS             <1..2217
                     /locus_tag="PKM23_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY90273.1"
                     /translation="GHFRGGRSNEVTYLVNGMQVTEAFGGSGRTVELETEAIQDLEVI
                     TGTFNAEYGRAMSGIVNAVTKSGSQRFSGSVSADLANYYTPHTDIFIGLDGGDVTRNQ
                     DYKFQLSGPVVPGWKSGPTFFVNGRYQDNKNHLNGVRRFNPGDLSNFISTEPMMWYSE
                     HSGDSAFVPMNNSLNQSLMAKLAFDMIRNVRMSLLYTRNDDEWHGYSHTWKYNPDGRA
                     TSYRTSDLLSFEFNHMISQKLFYEAKLSYLDNFNGYYLFRNPVDPGYLHDKFLTNDGP
                     GFYTGGQDKSHSLRWVKDTNAKFDVTWQATKNHALKSGILYTIHDLDQQSRTIRNKYY
                     GTDLEADLYEPVVYSDSSVYSDIYTVKPRELSAYIQDKIEYDEMVINFGLRYDYFDPN
                     TRYPSDLRNPANQLVFADSLNRKTDYIDAKPQVQISPRLGLSYQLSNAALLHFSYGHF
                     FQMPALYAIYDNHSFQVSPSDYATTMGNPQIKAQKTVQYEIGLWQELIKGMGLEVALF
                     YRDIYDLLSAKVVTTYDQIEYGLYSNKDYGNAKGLEVKYDFVTGSFSAYANYTLQYSR
                     GNADNPTTTFTRAGSSMDPISRLIPMSWDQRHTLNVTVGYNTPRFGSTLTAYYNSGAT
                     YTWSPLDESQLSKVNLYPNNAKMPSTYSFDMSAFYDLPLVNRFKARLTLNIYNLLDTL
                     NEWGVNGNTGRAYTAIVRASDLANHRSNFNTYYDRIQNPSMYATPRLIKLGLGIMF"
     gene            2267..5737
                     /locus_tag="PKM23_01995"
     CDS             2267..5737
                     /locus_tag="PKM23_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90274.1"
                     /translation="MKKSFLLAAFILLLTLSIVQAQGLLYDGPDDEAGDITATREGYL
                     SGNRVFTYFQNTTELGKWVSGATGGLWSKWPNNDSGTRMTDGIGLMIGAKVYVDNNSV
                     PVTDTTQIRSRTDLHDLYFLQTSYREEMDTDPTGTVEWGFYPCFGYFNEAQDYPAMTN
                     KPGSWPPAGWPAKGRSLKWPGEWDGRFGRGVIYAQQETYFVVNDAHDQEWLGPEDKYK
                     YYPRPGKKIGDIDPAKVTKQVGLPWGGLGIRVEQRGFQWSNPQARDCIFWEYAVANTS
                     DYDLPYVAFGYWVDNGIGGDNADDEFGYFNRKLDLAYSWDRDGLGGGGLPTGTMGFAY
                     LESPGMAYDGIDNDEDGLLDEKRDNGYPGDPTNALVGPTDNIYNLQAFLESFEYTLEE
                     LKPHYAADEDQDWEDGEDLNGDGIYQTSEYAGDDVGLDGVGPSELNYTGPDEGECNHR
                     PDYDGANGCEPNFAKTDVSESDMVGLTSFQMYPIPSHSSEEKWFRGDKSMWIVMSSDT
                     LVEYLGKVSNLVETFASGPFPLYQGRSERISMSELHSYDPLSGLTSPTHDAPALYEQK
                     KIVQIIYERDYRFASPPKMPTLQATAGDGKVILTWDNIADTRTKDPFVGNANDFEGYK
                     LFRATDKFFSDAMTITDGYGTPSFYNPIFQCDKKDSIQGFTDFGLVNGMGYYLGAETG
                     IVHFFTDNNVQNGRTYFYALVAYDYGAPQIEPGIAPSENEIVIETNEAEEIIAYGKNV
                     QIVVPRTTASGYSAPQISMDQSGVKFGAGTVTSTILAEASLKPGHDYQLKFLVDTLFA
                     VQDYANGLSYINNGLEVWDMTDNALVYTESPSRFAYDNLVYEDTLEYWYFNQDVPVTT
                     DIFEGLSLTLSDIPTTPRYDFQKSGWLTGSANMRVTLTSVETAYFPWDYDIVFTDNPN
                     VYTGRVTSKTMRNENNSRIDRTQLLVSQNFNFYVVNKSFLDSTGNYEKMDLVVHDVDK
                     DGKFDMLKDRILVGPVTSKGWWAGTAFILDFNNVADAANLPKPNDVYRITFQRPYMTS
                     DYISFKVLPQTEVDKSQITYSMANIKVVPNPYVATNAMEPSVSNWQLNQRRRLMFTHL
                     PAQCSIKIFTMSGVLVDEIDVQNDAANGMAQWDLLTSEGLEIAAGVYIFHVKSQLTGD
                     EFMSKFAVIK"
     gene            5826..6884
                     /locus_tag="PKM23_02000"
     CDS             5826..6884
                     /locus_tag="PKM23_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY90275.1"
                     /translation="MPFKNSIIILLAVLLGAGAVMAQKPYRIGTTAANFLEVGYGSAG
                     SAMGDAYVSVANDLSSAYWNPAGLAFMTRSEAQFSIQPWLVNINTTYAGVGLALPRVG
                     TLALAVTQVGYGDMAVTTMDYQDGTGELFNASDLAVSFSYARSLAQWFSFGASFKYIN
                     SQIWHMNANAMALDLGVMVNTNFFSFSGKREDGMTIGMSISNYGTKMKYDGMDLTWPI
                     DVLPDQDGNYAQTAGQFKLEGWELPLIFRVGVSIHPIATATNRLTLAVDALHPNNNAE
                     SINVGAQYAYTLPSTGTVYLRGGYKALALKETEFGPTMGAGVYLRMMGNLGVRFDYAY
                     RDIGMLGNAHCYSIGMSF"
     gene            7054..8553
                     /locus_tag="PKM23_02005"
     CDS             7054..8553
                     /locus_tag="PKM23_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90276.1"
                     /translation="MSFRRIPLLFLLLLALACERTRGPEENILVRVGDRTISKQEFIT
                     RAEYTIRPPYCRSDNYIHRKIVLNSLVAEKLYALEAGEKNELTENEQFQLYIQGRKEQ
                     AMRQWLYNEEFYGRVALDTAELKQEYKLAGRSYKLSYYSMPDSQMARLVGESLQQGKS
                     FEETFAQYGGLVTSIPTRELAWSGPEPEEFKARFFSAPLQKGQIIGPFEAERGLFTVA
                     RVDGWTDRLALGDQDVRQRWEDVREKVRTRQATAAYANWIGGLMRGKTLRFDGQTFPQ
                     VARVMADFYMKTEAEKKQLIKQQVWNVEDSSQVHPPVESLDGIADLPFMVLDDQVWTV
                     RDLQKLLLRHPLVFRSRQIPKGEFGLEFRNAIADMVRDLAVTEEAYKKGYDRVNVVQR
                     TAGMWRDNLLATWQRNRLLREKGREAEFYKEYQKVIESDLNPHFVELSKKYGKKIEIN
                     TDEFEKIKLTSIDMFVTEKNVPFPVVSPNFPLFTTHDLLDYGRKMNAGK"
     gene            complement(8608..9828)
                     /locus_tag="PKM23_02010"
     CDS             complement(8608..9828)
                     /locus_tag="PKM23_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNY90277.1"
                     /translation="MRKVKQLPENPEEMAQILMRMDARAAAVLLVKAEVESAARALRL
                     IGPQASEKILSQLAPDTRERLMQALPPAVREQWALNLTFPGESVGHLMDPVWALFTPE
                     MTVRQTIEELRQLVKQEFITYGYVTDAQKRLIGVVVMRELLFADPETPLREVMLTEPF
                     SLRASMPLMEAMREVLTRHFPIYPVTNDEGVLVGTVRGGRLFEAQAIEISAQAGSMVG
                     VEKEEKLSTAWPRSFRFRHPWLQLNLLTAFVAGAVVSVFQSTIDQIVVLAAFLPVLAG
                     QSGNSGCQALAVTIRGMTLGEYKKGQTRKLLAKESLLGLLNGSIIGLVAGGAMYLLAR
                     SQGNPMAFSLGLIVLAAMAGACVVSGIFGTLVPILLRRLGADPATASSIFLTTATDVA
                     SMGLLLLLATIFLV"
     gene            10364..10768
                     /locus_tag="PKM23_02015"
     CDS             10364..10768
                     /locus_tag="PKM23_02015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY90278.1"
                     /translation="MNEREFKRSLNSLEAVFDFIDKFLSRHNISGKSALELQLATEEV
                     FANAVEHNSSSTGVIAIALEKRGEAVMIRIRDHGVEDFDLSRPPEVDIRAPLNERRAG
                     GLGLYLLHSIMDQVKYTHESGISTITLVKYMK"
     gene            10775..11059
                     /locus_tag="PKM23_02020"
     CDS             10775..11059
                     /locus_tag="PKM23_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY90279.1"
                     /translation="MFKIEPNEQGVLHLSGRFDASQEEIAIRFFDQIAESTEVECSRL
                     EYISSAGIGVLIATQKRLMQRNEQLILTNLNPHIIEIFRYAGLDAIFSIR"
     gene            11107..11640
                     /locus_tag="PKM23_02025"
     CDS             11107..11640
                     /locus_tag="PKM23_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNY90280.1"
                     /translation="MIELQEAELVSRCRNGDDRAFAELVSCYQKPVYNLALRITRSGA
                     LAEEVTQSTFVKVYERFGSFKTGLSLFSWIYRIAMNEAINEYRRGKRRSSLDETRIMS
                     AAEPSFELAEAIQEALMQLKPDDRALIVLRHFQNLGYREIAVIMDRSESRIKSQLFRA
                     RSSLKIILARLGVTNEE"
     gene            11630..12688
                     /locus_tag="PKM23_02030"
     CDS             11630..12688
                     /locus_tag="PKM23_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90281.1"
                     /translation="MKSKTNHSLMQKALDGTLTEEERLAWQDWLQQHPGDLALYEEQA
                     LITRLLEKMPEEEPPAHLEGQIMRSIRNKQRNNHSIGQLIFQRQEDPMAPKYRWLLLG
                     AAAIVIIAVVISRFYPSTPDSTTQGTIGAVQKADKFDSGQMSAGDVTLTDSELQQLLQ
                     NEVVQRIVADQKLMAALQTMDSKALAALQTLDGRAIEALQSLDGRAIAAMQTLDARSI
                     AALQTLDGKAIAALQTLDAKSIAALQTVDAKSIPALQALDGKAIAALQSMDGKAIAAL
                     QTLDVKAIGALQSLDARSIAALQTVDARSIPALQSLDGKAIAALQSLDARSIAALHSL
                     DARTIASMQTAEGKATVE"
     gene            12889..15840
                     /locus_tag="PKM23_02035"
     CDS             12889..15840
                     /locus_tag="PKM23_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNY90282.1"
                     /translation="MLTKRHYASLLLLLLVLHTSDAAGQLSIQSTRRMDLVYFGRVHE
                     FIIPHLSRCYLNARSFESRLFDYHSDEKTTVYLQDFGDYGNAGAISVPHNFIRMELAP
                     HNYILETTTANERINWLMNHETVHVITTDQAAGSDHLFRALFCGKVQDDADDPVSLLY
                     AYLTTPRLLTPRWYLEGIAVFMETWLAGGLGRAQGSYDEMVFRAMVCDRAPFYNVVGL
                     EAEGTAIDFQVGANSYLYGARFVSYLGYEYGPEKVVEWYRRSEGSKRYFAAQFKKVFG
                     ASLADEWSRWIAWEHGWQQANLDSLRRQPVTPFRAISRRALGSVSRGFYDPDRNRLYT
                     ALLYPGQVAHIAALDLATGGLEPICEVKGPTLYSVTSLAWNPAAEALFYTSDNQRWRD
                     LNIVDIRTGRSRRLIKDGRIGDLAYDPTDSTLWGVRHFNGFSTLVAIPWPWREWKQVR
                     SFPYGQDIFDLALSPDGSILTASLVEISGRQKLIKIATAQPDTFEVLFDFENSLPQSF
                     VFSPDGRYSYGSSYYSGVSNIYRYDWARADMDILSNAETGFFQPVPLSDDSLLVMRYS
                     GRGFIPGKIAIQVPERVSAVTFLGAALAKEHPVVRSWKIGSPAHIAPDSLLQPAKPYQ
                     SLSYLRLGSAWPVVRGYKESAAIGYRANFMDLIGAAGMKVSALYSPDRSLPADERLHL
                     GIDGYWWQWNVSATLNHADFYDLFGPTKISRKGYALQAQYRKNLIYDKPRTLDLSASL
                     QHYGGMDRLPDYQNIAASYDRMSAARIALDYQYLRKSLGAVDDEKGIRLRAFTAASLV
                     NGRLYPRIYSHLDYGIPLALDHSSLWLRTSAGHCFGSSGNPFANFFFGGFGNNWVDRG
                     TEKRYREYFSFPGLELNALGGRSYGRAMLEWNLPPLRFSSLGITSLYCRWLRTAVFAS
                     AILTNPERAKGGQEMGALGIRRSLANLGIQADFRLVSLSRFDSTFSLGYAVALEGERK
                     NKEFMISLKIL"
     gene            15837..16841
                     /locus_tag="PKM23_02040"
     CDS             15837..16841
                     /locus_tag="PKM23_02040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNY90283.1"
                     /translation="MIFLHVILSLAPVVLLALLLQLLDSYKLVRGEAAATSLIKGAGA
                     ALVCMLINMLFIRKLHIEVTLYARYIAPFLEEGAKALLVYGLIRKKQVGFLVDAAIHG
                     VAMGAGFAVIENIYYLYSLAESSLMIWIIRGFGTAVMHASCTALFAILCKHLHDRNGR
                     LGISCWGPAWFVAALIHSAFNHFILSPLLSTLVILAILPVFIILVYEYSERSLRKWLA
                     LSMDEEMAMLEIILSGRFTESKFGQYLLSLRDHFPPAVVVDLLCYLRIYLELAMRAKG
                     ILLLRQHGLPAQPDTALREKFNEFKYLEKSIGRTGKLALVPFMRIRDRELWQLYMLEK
                     "
     gene            16991..17371
                     /locus_tag="PKM23_02045"
     CDS             16991..17371
                     /locus_tag="PKM23_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY90284.1"
                     /translation="MKVMVVDDERDIELLFRQHFRKEIRQQQIDFSFALSGEEALQQL
                     RDMRQPPAVVLILSDINMPGMNGLQLLKAVKHDFPAIRVAMITAYSNQYYEDAMASGA
                     DHYYTKPIDFTQLKNELFMEAVHG"
     gene            17364..21104
                     /locus_tag="PKM23_02050"
     CDS             17364..21104
                     /locus_tag="PKM23_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY90285.1"
                     /translation="MAKNRLLILLLLGAIAHAAETGMPFMTVYPSKEFGGHTQFWSIE
                     QDKRGLMYIGDGYGIQEFDGANWRLIINANHTFGRSLSRDENGRIYAGSSAMLGYLAA
                     DERGEMQYCSLMEYIKPEDRQFNYVWSVQATREGIYFQTNERLFRFHPPESGAAAGVW
                     TVDVWRPQELFGYTFWLDQTLYVQQYGVGLMKMEQDSLVLLPGGGQFADDRINVMLPF
                     PGKAGHYLVGTFTRGLFLWDGTTFQPLPTEVDNLLRQGTIYAGVVTPDSCFALGTMAN
                     GLVIIDRDGRMRLHFSQTNGFLSNTVSCLYMDRQNNLWVGMDGGVAVLEYNSPLSEFI
                     IPGGSGPSDFCRYHGVLYITCNDGVYYLDEVDGRFKMIDGIAGNAQSFFFFQINGELF
                     VTVNEGIYRIQGKKPHLVMPSREMSVPVLHMSALSLAPDLIIAGKSSGADLLRYNASH
                     PQRLSWLGSIPEVYEYIRQAMEAEPGVVWLGTMDAGLIRLTFNKDRLLDPVIERFGME
                     HGLPPGGAVFYNPGGRPVFCTKKGVYQFNPAQNRFSPDPFFKDLGLGINPDESIIVAD
                     AQGNFWANLGKESAVYKKLPDGQYKLEKAQLARFVDEVINIIFPESDGVIWFGAANRV
                     IRFSHGLQERDLTDFPAIIRRVALAGDSVVYYGAALPGHRLQDPAAQRFPFRNNALRF
                     DFSASSYLNPRANAFRTRLEGFDAGWSSWSRETRRDYTNLPAGKYRFRVQARNIFQQE
                     SKEDLYAFTITPPWYRSWWAWLFYLLCAGGLIAGLVRVRTRQLEERGRALEKIVQERT
                     AEIQAQKNNVEKLSLIGREITATLSIGNIIHTIYENVNQLMDASVFGIGLYNADQQTL
                     DFPGTTEKNELLAPYTLSLSDENRLAVWCFKNQKEVIINDYAEDFSAYIQQLQAPVAG
                     ENPESILYLPLTYKDKRIGVITAQSFNKNAYTDYHLNMLRNLATYSAIALENADAYRQ
                     VNELLADLKNTQEKLVTQSKLAALGALTAGIAHEIKNPLNFVNNFAELITDLVAELRE
                     VLTAEMGEAATAKKNEAMEILDTLQQNGAKIIEHGKRADSIVKSMLQHSRGKSDERQP
                     TDINAVLAEDINLAYHGMRAQDSTFNIKIETDFDPAVGKLNIVPQDVSRVFLNIITNG
                     CYEAHRKKQEQNGEFSPTLSVKTRNLANLVEVRIRDNGNGIPAGVREKLFTPFFTTKP
                     PGKGTGLGLSISYDVIVHQHGGQLFFESEEGRFAEFIIHLPK"
     gene            21127..22293
                     /locus_tag="PKM23_02055"
     CDS             21127..22293
                     /locus_tag="PKM23_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNY90286.1"
                     /translation="MPAKILVVDDEPDLEQLILQKFRKQIREGAFTFKFAGNGVQALE
                     RLAEDEAIDLVLTDINMPEMDGLTLLNRIKEQENPLLRSVIVSAYGDIENIRTAMNRG
                     AFDFIIKPIDLNDLEITIDKSLNDLRVLKEAMQSRDQLLFIKHELGIATEIQTSILPK
                     MFPPFPDRKEFDIYAKMIPAREVGGDLYDFFMIDKARVGVIIGDVSGKGIPAAMFMAV
                     SKTLLKATALKGIPPDSCLESVNKVLVDESLPSMFVTVFYGIFDTRNGSFEYCNGGHN
                     PPYLISAEGKVRQLDNRGGVFLGALKNLEYESNVVMLQPGETLFLITDGITEAMNPGE
                     EMFGEKRTEESLQSHAGESLNNLVENMIKEVQAFAGAQEQSDDMTCLTLRYFGR"
     gene            22630..>22892
                     /locus_tag="PKM23_02060"
     CDS             22630..>22892
                     /locus_tag="PKM23_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNY90287.1"
                     /translation="MTINDGLSNNSINDILQTHDGYIWIATKDGLNCYDGQHFKVFKH
                     DPIDTNTVPENYVYCLLESRDSTLWVGTWGGGQPTGCYLCFRA"
BASE COUNT         4920 a         7156 c         6098 g         4718 t
ORIGIN      
        1 ggccacttcc gcggcggccg cagtaatgaa gtcacctatc tggtcaacgg catgcaggtc
       61 accgaggcct tcggcggcag cggccgcacc gtcgaactcg agaccgaggc catccaggac
      121 ctcgaggtca tcaccggcac cttcaacgcc gaatacggcc gcgccatgag cggcatcgtc
      181 aacgccgtca ccaaatcggg cagccagcgc ttcagcggat cggtctccgc cgacctcgca
      241 aactattaca ccccccatac cgatatcttc atcggcctcg acggtggcga cgtgacccgc
      301 aaccaggatt acaaattcca gctcagcggc ccggtggtcc ccggctggaa atcgggcccg
      361 actttcttcg tcaacggccg ctatcaggac aacaagaacc acctcaacgg cgtgcgccgc
      421 ttcaatcccg gcgatctcag caatttcatc tccaccgaac cgatgatgtg gtactccgag
      481 cactccggcg acagcgcctt cgtgcccatg aacaacagcc tcaaccagtc gctcatggcc
      541 aagctggcct tcgatatgat caggaatgtc cgcatgtcgc tgctctatac ccgcaatgat
      601 gacgaatggc acggctattc gcacacctgg aaatataacc cggacggacg ggccacttcc
      661 tatcgcacct cggatctgct ctcgttcgag ttcaaccaca tgatcagcca gaagctcttt
      721 tacgaagcca aactctccta tctggacaat ttcaacggct actacctttt ccgcaatcct
      781 gttgacccgg gttatctgca cgacaagttc ctcaccaacg acgggcccgg tttctacacc
      841 ggcggacagg acaagagcca ttccctgcgc tgggtcaagg acaccaacgc caagttcgac
      901 gtcacctggc aggcaaccaa aaaccacgcc ctcaaaagcg gcatcctcta taccattcat
      961 gacctcgatc agcagtcgcg gaccatccgc aacaaatatt acggcaccga cctcgaggcc
     1021 gacctctacg aaccggtcgt ctattccgac tcctcggtct attctgacat ctacaccgtc
     1081 aagccgagag aactctcggc ctacatccag gacaagatag aatacgacga gatggtgatc
     1141 aatttcggcc tccgctatga ctatttcgat cccaacaccc gctatccctc ggacctgcgc
     1201 aatccggcca accagctcgt cttcgccgac agcctcaacc gcaagaccga ctatatcgac
     1261 gccaaacccc aggtgcagat cagcccgcgg cttggcctct cttatcagct cagcaacgcg
     1321 gccctgctcc atttcagcta cggccatttc ttccagatgc cggcgctcta cgccatctac
     1381 gacaaccact ccttccaggt ctcgccctcc gactatgcca ccaccatggg caatccccag
     1441 atcaaggccc agaagaccgt gcagtacgag atcggcctct ggcaggaact gatcaagggg
     1501 atggggctgg aagtggccct cttttaccgc gatatctacg atctgctcag cgccaaggtg
     1561 gtcaccacct acgaccagat cgaatacggc ctttacagca acaaggacta tggcaacgcc
     1621 aagggtctgg aggtcaagta cgattttgtc accggcagct tttctgctta cgccaactat
     1681 accctgcagt acagccgcgg caacgccgac aatccgacga ccaccttcac ccgcgccggc
     1741 agcagcatgg atccgatcag ccgcctgatc ccgatgagct gggaccagcg tcataccctc
     1801 aacgtgaccg tgggctacaa taccccgcgc ttcggctcca ccctgaccgc ctactacaac
     1861 tcgggcgcca cctatacctg gtcgcccctg gacgagagcc agctctccaa ggtcaatctc
     1921 tatcccaata atgccaagat gcccagcacc tacagcttcg acatgagcgc cttctacgat
     1981 ctgccgctgg tcaaccggtt caaggcccgt ctgaccctca atatctacaa tctcctcgat
     2041 accctcaacg agtggggcgt caacggcaac accggccgcg cctataccgc catcgtgcgt
     2101 gcatcggacc tggccaatca tcgcagcaac ttcaacacct attacgatcg catccagaat
     2161 ccatcgatgt atgccacccc gcggctgatc aagctcggcc tgggcatcat gttctaagcc
     2221 gccccccggc ttatttcagg acggataaca gataaggaac ttgtgcatga aaaaatcatt
     2281 tctgctcgct gcgttcatcc tgctcttgac tctatcaatc gtccaggccc agggtctgct
     2341 ttacgatggc ccggatgatg aggcgggcga tatcaccgcc acacgcgagg gctacctgag
     2401 cggcaatcgc gttttcacct attttcagaa taccaccgaa ctgggcaaat gggtcagcgg
     2461 cgccaccggc ggcttgtggt ccaagtggcc caacaacgac agcggcacgc gcatgaccga
     2521 cggcatcggc ctgatgatcg gtgccaaggt ctatgtcgac aacaactcag tgccggtcac
     2581 cgacaccacc cagattcgct cccgtaccga cctccacgat ctctatttcc tccagaccag
     2641 ttaccgcgag gagatggata ccgaccccac cggcaccgtg gagtggggtt tttatccctg
     2701 cttcggctat ttcaacgaag cccaggacta tcctgcgatg accaacaagc ccggttcctg
     2761 gcctcctgcg ggctggccgg ccaagggccg cagcctcaaa tggccgggcg aatgggacgg
     2821 ccgcttcggc cgcggcgtca tctatgccca gcaggagacc tacttcgttg ttaacgacgc
     2881 ccacgaccag gagtggctcg gtcccgaaga caagtataaa tactatcccc gtcccggcaa
     2941 aaagatcggc gacatcgatc ccgccaaggt gaccaagcag gtcggcctgc cctggggcgg
     3001 cctcggcatc cgcgtcgaac aacgcggctt tcagtggtcc aacccccagg cgagggactg
     3061 catcttctgg gaatacgccg tcgccaacac ctccgattat gaccttccct acgtcgcctt
     3121 tggctactgg gtcgacaacg gcatcggcgg cgacaacgcc gatgacgagt tcggctattt
     3181 caaccgcaag ctcgacctcg cctactcctg ggaccgtgac ggcctcggcg gcggcggcct
     3241 gcccaccggc accatgggct tcgcctacct cgagagcccc ggtatggcct acgacggcat
     3301 cgacaacgac gaggacggtc tcctcgacga aaaacgcgac aacggctatc ccggcgaccc
     3361 caccaacgcc ctggtcggcc cgaccgacaa catttacaat ctccaggcct tcctcgagag
     3421 tttcgagtac accctcgaag agctcaagcc ccactatgcg gccgatgagg atcaggactg
     3481 ggaggacggc gaggacctca acggcgacgg catctaccag accagcgaat atgccggcga
     3541 tgatgtcggc ctcgatgggg tcggcccctc cgaactcaac tataccggcc cggatgaggg
     3601 cgagtgcaac cacagacccg actatgacgg cgccaacggc tgtgaaccca acttcgccaa
     3661 gaccgacgtc agtgaatccg acatggtcgg tctgacctct ttccagatgt atcccatccc
     3721 gtcgcactcc tccgaggaga agtggttccg cggcgacaag tcgatgtgga tcgtcatgag
     3781 ctccgacacc ctggtcgaat acctcggcaa ggtctccaac ctcgtcgaaa cctttgcctc
     3841 cggccccttc cccctctacc agggacgctc cgagcgcatc tccatgagcg agctccactc
     3901 ctacgatccc ctgagcggac tgacttcccc cactcatgat gccccggcgc tctacgagca
     3961 gaaaaagatc gtccagatca tctatgagcg cgactaccgc ttcgcctcac ccccgaagat
     4021 gccgaccctt caggccaccg ccggtgacgg caaggtcatc ctgacctggg acaatatcgc
     4081 cgacactcgc accaaggatc cctttgtcgg caacgccaac gactttgagg gctacaagct
     4141 cttccgcgcc accgacaagt tcttctccga tgccatgacc atcaccgacg gctacggcac
     4201 gcccagcttt tacaatccca ttttccagtg cgacaagaag gactcgatcc agggattcac
     4261 cgatttcggc ctcgtcaacg gcatgggcta ctacctcggc gctgagaccg gtatcgtcca
     4321 ctttttcacc gacaacaacg tccagaacgg gcgcacctat ttctacgccc tcgtcgccta
     4381 cgactacggc gcgccccaga tcgagcccgg catcgcccct tcggaaaacg agatcgtcat
     4441 cgaaaccaac gaagccgaag agatcatcgc atacggcaag aacgtccaga tcgtcgtgcc
     4501 gcgcacaacc gcctcgggct attccgcacc ccagatctcc atggaccaga gcggggtcaa
     4561 gttcggcgcc ggcaccgtca ccagcaccat cctcgccgaa gccagcctca agcccggcca
     4621 cgattaccag ctcaaattcc tggtcgacac cctcttcgcc gttcaggatt atgccaacgg
     4681 tttgtcctat atcaacaacg gcctcgaggt ctgggatatg accgataatg ccctggttta
     4741 caccgaatcg cccagccgtt ttgcctatga caacctggtc tacgaagaca ccctggagta
     4801 ctggtacttc aatcaggatg tcccggtcac gacggatatc tttgagggat tgagcctgac
     4861 cctctccgat atcccgacca ctccccgcta cgatttccag aaatcgggct ggctgaccgg
     4921 ttccgccaac atgcgcgtca ccctgacctc ggttgagacg gcctatttcc cctgggatta
     4981 tgacattgtt tttactgaca atcccaatgt ctataccggc cgcgtcacca gcaagaccat
     5041 gcgcaacgag aacaacagcc gcatcgaccg cacccagctg ctggtctcgc aaaactttaa
     5101 cttttacgtg gtcaacaagt ccttcctcga ctccaccgga aactatgaaa aaatggacct
     5161 ggtggtgcat gatgtcgaca aggatggcaa gttcgacatg ctcaaggatc gcatcctggt
     5221 cggcccggtt acctcaaagg gctggtgggc cggaaccgcc ttcatcctcg atttcaacaa
     5281 tgtcgcggat gcggccaacc tgcctaaacc caatgacgtc taccgcatca ccttccagcg
     5341 gccctacatg acgagcgact atatctcttt caaggtcctt ccccagaccg aggtggacaa
     5401 gtcccagatc acctactcta tggccaacat caaggtggtg cccaatccct atgtcgccac
     5461 caacgccatg gagccttcgg tgagcaactg gcagctcaac cagcgccgcc ggctgatgtt
     5521 cacccatctg cccgcccagt gctcgatcaa gattttcacc atgagcggcg ttctggtcga
     5581 cgaaatcgat gtccagaatg acgcggccaa cggcatggcc cagtgggacc tgctcaccag
     5641 cgaggggctc gaaatcgccg ccggcgtcta tattttccac gtcaagtccc agctgacggg
     5701 cgacgagttc atgagcaagt tcgcggtgat caagtgagtg caccccgccg gatcccggca
     5761 gcgggatccg gcggctgcag catccccagg ggtgcggacc ttaaaacgta tacgaggtaa
     5821 ctgatatgcc atttaaaaat tcgatcatca tcctgctggc cgtgctgctc ggcgccggtg
     5881 ccgtcatggc gcaaaagccc taccgcatcg gcaccaccgc cgccaacttt ctcgaggtcg
     5941 ggtacggcag cgccggctct gccatgggcg acgcctatgt cagcgtcgcc aacgacctct
     6001 cctcggctta ctggaatccg gccggcctgg ccttcatgac ccggagcgaa gcccagttct
     6061 ccatccagcc ctggctggtc aacatcaaca ccacctatgc cggggtcgga ctcgccctgc
     6121 cgcgcgtagg caccctcgcc ctcgctgtca cccaggtcgg ctacggcgac atggccgtga
     6181 ccaccatgga ttatcaggac ggcactggtg agttgttcaa cgcttcggac ctggcggtga
     6241 gcttttccta cgcccgcagc ctggcgcagt ggttttcctt cggcgcctcc ttcaaatata
     6301 tcaactccca gatctggcac atgaatgcca acgccatggc cctcgacctc ggcgtcatgg
     6361 tcaataccaa ctttttctcc ttcagcggca aacgcgaaga cggcatgacc atcggcatga
     6421 gcatctccaa ttacggcacc aagatgaaat atgacggcat ggacctcacc tggccgatcg
     6481 atgtcctgcc ggatcaggat ggcaattacg cccagaccgc cggccagttc aagctcgagg
     6541 gctgggaact gccgctgatc ttccgcgtcg gcgtctcgat ccatccgatc gcaaccgcca
     6601 ccaaccgcct caccctggcg gtggatgccc ttcatcccaa caacaacgcc gagtccatca
     6661 acgtcggcgc ccagtacgcc tataccctgc cctccaccgg caccgtttat ctgcgcggcg
     6721 gctacaaggc cctggccctt aaagagaccg aattcgggcc gaccatgggc gccggggtct
     6781 acctgcgcat gatgggcaac ctcggcgtcc gctttgatta tgcctatcgc gatatcggca
     6841 tgctgggcaa tgcccactgc tacagcatcg gcatgagttt ctgaacggat gggctgcccg
     6901 cccccgggcg ggcagctctc catctcgttc ctacccgcca gcgggcagcc ctccatctcg
     6961 ttcccacccg cctgcgggcg ccgctccaga tctcgttccc accttttggc cccggtggga
     7021 acgctcattt atcaacaaga gagcccctgt tttatgagtt tcagacgaat cccccttctc
     7081 tttctgctcc ttctcgccct tgcttgtgaa cgcacccggg gaccggagga gaacattctc
     7141 gtccgcgtcg gcgaccgcac catttcgaag caagagttca tcacccgcgc agagtacacc
     7201 atccgtccgc cctactgccg gagcgacaac tatatccacc gcaagattgt cctcaattcc
     7261 ctcgtcgccg aaaagctcta cgcccttgag gccggtgaga agaatgagct gactgaaaac
     7321 gaacagttcc agctctatat ccagggtcgc aaggaacagg cgatgcgaca atggctttac
     7381 aacgaagaat tctatggcag ggtggcactg gataccgccg agctgaagca ggagtacaaa
     7441 ctggcgggga gaagctacaa actctcctat tactcgatgc cggacagcca gatggcgcgg
     7501 ctggtggggg agagcctgca gcagggcaag agcttcgagg agacctttgc gcagtacgga
     7561 ggcctggtca cctccattcc gacgcgggaa ctggcctggt ccggccctga acccgaggag
     7621 ttcaaggcgc gttttttctc ggctcccctg caaaagggcc agatcatcgg ccccttcgag
     7681 gcagagagag gattgttcac agtcgcccgg gtggatggat ggaccgaccg cctcgccctc
     7741 ggcgaccagg atgtccggca gcgctgggag gatgtcaggg aaaaggtgcg cacccgccag
     7801 gccacggcgg cctatgccaa ctggatcggg ggcttgatgc gcggcaagac cctgcgcttc
     7861 gacggccaga cctttcccca ggtcgcgcgg gtgatggcgg acttttatat gaagaccgag
     7921 gcggagaaaa agcagctgat caaacagcag gtctggaatg tggaggacag ctcgcaggtc
     7981 catccgccgg tggagagcct cgacgggatc gccgacctcc ccttcatggt gctcgatgac
     8041 caggtctgga cagtgcgcga tctgcaaaag ctgcttctgc gccatcccct ggtcttccgc
     8101 agccgccaga tccccaaagg ggagttcggc ctcgagttcc gcaacgccat cgccgacatg
     8161 gtgcgcgacc tggccgtcac cgaagaagcc tacaagaaag gctatgaccg ggtcaacgtc
     8221 gtccagcgca cggccgggat gtggcgggac aatctcctcg ccacctggca gcgcaaccgg
     8281 ctcctgcgcg aaaaggggag ggaggccgag ttttacaaag agtaccagaa ggtgatcgag
     8341 agcgacctca atccccattt tgtcgagctt agcaagaaat acggcaagaa gatcgagatc
     8401 aataccgacg aattcgaaaa gatcaagctg acgagcatcg acatgttcgt cactgaaaag
     8461 aatgttccct ttccggtggt ttcgcccaac tttcccctgt tcaccacgca tgatctgctc
     8521 gactacggcc gcaagatgaa tgccgggaaa tagtacaccc ggctggacgc accgtgccgc
     8581 cgcggggtgc ggcggcgggc cggtaactca gaccagaaag atcgtggcga gcaggagcag
     8641 cagtcccatg ctggcgacat cggtcgccgt cgtcaggaaa atgctcgagg cggtggccgg
     8701 gtcagccccc agccgccgca gcagaatcgg taccagcgtg ccgaagatgc cgctgaccac
     8761 gcaggccccg gccatggccg ccaggacgat caatcccagc gaaaaggcca tcggattgcc
     8821 ctgactgcgc gccagcagat acatcgcccc gcccgccacc agaccgatga tgcttccgtt
     8881 caacagcccc agcagactct ccttggccag cagtttcctt gtctggcctt ttttatattc
     8941 ccctagggtc atgccgcgta tcgtcaccgc cagggcctgg cagccgctgt tgcccgactg
     9001 gcccgccagc accggcagaa aggcggcgag gacgacgatc tggtcgatgg tcgactggaa
     9061 aacgctgacc acggccccgg cgacaaaggc ggtgagcagg ttgagctgca gccagggatg
     9121 gcggaagcgg aagctgcgcg gccaggcggt ggagagcttt tcctcttttt cgacgccgac
     9181 catgcttccg gcctgggcgc tgatctcgat ggcctgggcc tcgaagaggc ggccgccgcg
     9241 gacagtcccg accagcaccc cctcgtcgtt ggtgaccgga tagatgggaa agtgcctggt
     9301 gagcacctcc cgcatcgcct ccatcagggg catggaggcg cgcagactga agggctcggt
     9361 cagcatgacc tcgcgcaggg gcgtctccgg gtcggcgaag aggagctcgc gcatcaccac
     9421 caccccgatc aggcgcttct gcgcatcggt gacatagccg taggtgatga actcctgctt
     9481 gacgagctgg cgcagttcct cgatggtttg gcggacggtc atttcgggcg tgaaaagagc
     9541 ccagaccgga tccatgaggt ggccgacgct ctccccgggg aaggtcaggt tgagtgccca
     9601 ctgctcgcgc accgcgggcg gcagggcctg catgagcctt tcccgggtgt cgggtgcaag
     9661 ctgggagagg attttttccg aggcttgtgg cccgatgaga cgcagcgccc gggccgcgga
     9721 ttcaacctcc gctttcacca gcagaacggc cgccgccctg gcgtccatcc gcatcagtat
     9781 ctgggccatc tcttcgggat tctctggaag ctgtttcact tttctcatgt ttattccctg
     9841 aaattggact tttaactgcc ttgcctttac agttgataca ccgctcaaag ctctctaacg
     9901 ggaaaagata gataaatgag gggataatat caagtcaaaa ggccaggtcc gcatcctatc
     9961 caatcgaatt gtggtatcca gcatcaaaca ccccagcagc aaagcaatat gctcgctcct
    10021 gctcaacctg caagcctgct gtttctgtac atgatgtttt ccctcacacg aagtatgggt
    10081 ggtcaaatta cccggttcgg gggcggattt tgcttgcacc gtactctttt tttggatata
    10141 atgagtatag tcggcttgaa cacctccaga cgggccatac aggagtaaat cggaccgccg
    10201 gtgttgtatt ccctttcatc gctcgatgaa cggctccacc ttgtcgataa tcgagtctgt
    10261 cccaatccac cattccgggc cgcgttgaca tgcgccggaa tgaatgcttt cctgctttat
    10321 cctattcgct atgttacaat ctctgttgcc ttcggaagaa ccgatgaatg agcgtgaatt
    10381 caagcgatca ctgaactccc tcgaagcagt gtttgatttt atcgataaat tcctttcccg
    10441 ccacaacatc tccggcaaat cagcgcttga gctgcagctg gccacggagg aggtatttgc
    10501 caacgcggtc gagcacaaca gcagctcgac cggcgtcatt gccattgcac tggagaaaag
    10561 gggagaggcc gttatgatcc gcatccgcga tcatggtgtg gaggatttcg acctctcccg
    10621 cccgcctgag gtggatatcc gggccccttt gaacgagcgc cgggcgggcg gcctgggttt
    10681 gtacctgctc cattccatta tggaccaggt caaatacaca cacgagagtg gaatcagcac
    10741 gatcactctg gtcaaatata tgaagtgagg aagcatgttc aaaatcgaac ccaatgagca
    10801 gggcgtcctg cacctgagcg gacgttttga tgcttcacag gaggagatcg cgatccggtt
    10861 tttcgatcag atcgcggaaa gcacggaggt cgaatgcagc cggctggaat atatctccag
    10921 cgccggcatc ggtgtgctca tcgcgactca aaaacggctg atgcaaagaa atgagcagct
    10981 gatccttaca aacctcaatc cgcatatcat tgaaattttc aggtatgcgg gactggatgc
    11041 cattttctca atcagataaa atttttgtat gaaaccgggc aactttcaca tctttggatt
    11101 gtatgtatga ttgaactgca agaagctgag ctggtcagcc gctgcagaaa tggcgacgat
    11161 cgcgcctttg cggagttggt cagctgctac cagaaaccgg tctacaatct ggctttgcgc
    11221 attacccgca gcggcgcgtt ggccgaggag gtgacccagt ccacttttgt caaggtatac
    11281 gaaaggttcg gttctttcaa aacggggctc tctctattca gctggatcta tcgtatcgca
    11341 atgaacgagg cgatcaacga gtaccggcgc ggcaaacggc gcagcagtct ggatgagacg
    11401 cgcataatga gcgcggcgga accctccttt gagttggcgg aggccatcca ggaggccctg
    11461 atgcaattaa agccggatga ccgggcgttg atcgttttgc ggcattttca gaatctcggc
    11521 tatcgggaga tcgccgtcat catggatcgc tcggaaagcc ggataaagtc acagcttttc
    11581 cgggcgcgca gcagcctgaa gatcattctc gccagattgg gtgtaaccaa tgaagagtaa
    11641 aaccaaccat tcattaatgc aaaaggccct cgacgggacg ctcactgaag aggagcggct
    11701 cgcatggcag gattggctgc agcagcatcc cggcgatctg gcgctgtacg aagagcaggc
    11761 cctgattaca cggcttctgg agaagatgcc ggaggaagaa ccgccggccc atctcgaagg
    11821 ccagatcatg cgctcgatcc gaaataagca acgtaataac cattccatag gacaactaat
    11881 ttttcaacga caggaggatc ccatggcacc caagtacaga tggcttttat taggcgccgc
    11941 cgcgattgtg atcatcgctg tggtaatctc ccggttttat ccctcgacgc cggattccac
    12001 tacccaggga accatcggcg ctgtacagaa agccgacaaa tttgattccg ggcaaatgag
    12061 tgccggcgat gtgaccttga ccgatagcga gctgcagcag ctgctgcaga atgaggtggt
    12121 acaaagaatc gtcgctgatc agaagttgat ggctgcgctg caaacgatgg acagcaaggc
    12181 gctcgcagcc ctgcagactc tcgatggccg ggccattgag gcgctgcagt ctctggatgg
    12241 ccgggcgatt gcagccatgc agaccctcga tgccagatcc attgccgccc tgcagactct
    12301 ggacggcaaa gccattgccg ccctgcagac cctggatgcc aaatccattg ccgctttgca
    12361 gaccgttgac gccaaatcga ttccggccct ccaggcgttg gatggcaaag cgatcgccgc
    12421 cctgcagagc atggatggca aggccattgc cgccctgcag accctcgacg tcaaggcgat
    12481 cggcgcattg cagtccctgg atgccaggtc catcgccgcc ctgcagaccg tcgatgcccg
    12541 gtctatcccc gcgctccagt cgctggatgg caaggcgatc gcggccctgc aatccctgga
    12601 tgccaggtcc atcgctgctc tgcatagtct cgacgccaga accatcgcca gcatgcaaac
    12661 ggcggaaggc aaagccacag tcgaataact ccagcctgag atctctccgc aggccggcag
    12721 gaatcccggt actctccgcc cgcggattaa aatcgccgca cttgctgagg cccaactttt
    12781 ctatacggag gcgctcctgc cggggcgcct ccgttcgaac tctgccatga ttcctgagga
    12841 gattctttct cgcaagctgc tcatccacag gtagaggctg ttgaacagat gctgacgaaa
    12901 agacactacg cctcgttact gctcctcctg ctggtacttc atacctctga cgcagccggc
    12961 cagctcagca tccaatccac caggcgaatg gatctggtct atttcggcag ggttcacgag
    13021 ttcattatcc cccatctctc gcgctgctac ctgaatgccc gcagtttcga gagccggctc
    13081 ttcgactatc acagcgatga aaaaaccacc gtttacctcc aggatttcgg ggattatggc
    13141 aatgcagggg ccatctccgt accccataat tttatccgca tggagctcgc tccccacaat
    13201 tacatcttag agaccacaac ggccaacgaa cgcatcaact ggctgatgaa ccatgagact
    13261 gtgcacgtca tcaccaccga tcaggcggcc ggcagcgacc atctctttcg cgctcttttt
    13321 tgcggcaagg tgcaggacga cgcggacgat ccggtctccc tcctctatgc ctatctcacc
    13381 acaccgcggc tgctgacccc gcgctggtat cttgagggca tagccgtttt catggagacc
    13441 tggctggcgg gcggcctggg acgggctcag ggttcctatg acgaaatggt cttccgcgcc
    13501 atggtctgcg accgggcccc tttttacaat gtggtcgggc tcgaagcgga ggggacggcg
    13561 atcgattttc aggtcggcgc caattcctat ctctacggcg cccgtttcgt cagctatctc
    13621 gggtatgaat acggcccgga gaaggtggtg gagtggtata gacggagcga gggcagcaag
    13681 aggtatttcg cagcgcagtt caaaaaggtc ttcggggcga gcctcgccga tgagtggtcc
    13741 cggtggatcg cctgggaaca tggctggcag caggccaatc tcgattctct ccgccgccaa
    13801 cccgtgactc ccttccgggc gatctcccgg cgtgccctcg gctcggtctc gcgcggtttt
    13861 tacgatcccg accgcaacag attgtacact gcactcctct atcccggcca ggtcgcccac
    13921 atcgccgccc tggacctggc cactggcggc ctcgagccca tctgcgaggt caaaggcccg
    13981 accctctact ccgtaacctc gctggcctgg aatcccgctg ccgaggccct cttttacacc
    14041 agcgacaatc agcgctggcg cgatctcaac atcgtcgata tcaggaccgg ccgctcgcgg
    14101 cgtctgatca aggacggtcg catcggcgac ctcgcctatg accccaccga cagcaccctc
    14161 tggggcgtac gccactttaa cggcttttcc accctggtcg ccatcccctg gccctggcgg
    14221 gaatggaaac aggtccgctc ctttccctac ggccaggaca tcttcgacct tgctctttct
    14281 cccgatggat cgatcctcac cgcctcgctg gtcgaaatct ccgggcggca gaagctgatc
    14341 aagatcgcca ccgcccagcc tgacacgttt gaggtgctct tcgatttcga aaacagcctg
    14401 ccgcagagtt tcgtcttttc accggacggc cgctacagct acggctcctc ctattattcc
    14461 ggggtctcca atatctatcg ctacgactgg gccagggcgg acatggacat cctcagcaac
    14521 gccgagaccg gtttttttca gccggtcccg ctctctgacg actccctgct ggtcatgcgc
    14581 tacagcggcc gcggtttcat cccgggcaag atcgccattc aggttccgga gcgggtgagc
    14641 gccgtcactt ttctcggcgc cgccctagcc aaagaacatc cggtcgtgcg cagctggaag
    14701 atcggttctc ctgctcatat tgcgcccgat tcgctgcttc aacctgccaa accatatcaa
    14761 tctctcagct acttgcgcct gggctcggcc tggccggtcg tccgcggcta taaggagagc
    14821 gccgccatcg gttaccgcgc caatttcatg gatctcatcg gcgcagccgg catgaaggtc
    14881 tcggccctct acagcccgga tcgcagtctg cccgccgacg agcggctcca tctggggata
    14941 gatggatact ggtggcagtg gaacgtcagc gccactctca accatgccga tttctacgac
    15001 cttttcggtc cgaccaaaat cagccgcaag ggctatgccc tgcaggcgca gtaccgcaag
    15061 aacctgattt acgacaaacc ccgcaccctg gacctctccg ccagcctcca gcattacggc
    15121 ggcatggatc gtctgccgga ttaccagaac atcgcggcct cctacgaccg catgtccgcc
    15181 gccaggatcg ccctggatta ccagtacctg cgcaagtctc tcggtgcggt cgacgacgaa
    15241 aagggaatcc gcctgcgggc tttcacagca gcctctctcg tcaacggccg gctctacccg
    15301 cgtatctaca gtcacctcga ttatggcatc ccgctggcgc tcgatcactc ttccctctgg
    15361 ctgcgcacat cagccggtca ttgcttcggc agctccggga accccttcgc caattttttc
    15421 ttcggcggtt ttggcaacaa ctgggtcgac cgtggaacgg aaaaacgcta tcgggaatac
    15481 ttcagttttc cgggcctcga gctgaatgcc ctggggggca ggagttatgg cagggccatg
    15541 ctcgagtgga atctgccgcc cctgcgcttc agcagccttg gcattaccag cctctactgc
    15601 cgttggctgc ggactgcggt gttcgcctcc gccattctga ccaatcccga acgcgccaaa
    15661 ggcggccagg aaatgggcgc gctggggatt cgccgcagcc tggccaatct cggcatccag
    15721 gccgatttcc gcctggtgag tctgtcgcgc tttgattcga ccttttcttt ggggtacgcc
    15781 gttgccctcg agggcgaaag gaaaaacaag gaattcatga tctctctgaa aattttatga
    15841 tcttcttgca cgtcatcctc agcctcgctc cggtcgttct tctcgccctg ctcctccagc
    15901 tgctggacag ctacaaactg gttcggggag aggcggccgc caccagtttg atcaagggcg
    15961 ccggagcagc cctggtctgc atgctgataa atatgctctt catccgcaag ctgcatatcg
    16021 aggtgaccct ttatgcccgc tatatcgccc ccttcctcga ggagggggca aaagccctgc
    16081 tcgtctatgg ccttatccgt aaaaaacagg tcggttttct cgtcgatgcc gccatccacg
    16141 gcgtggcgat gggagcggga tttgccgtga tcgaaaatat ctactatctc tacagcctgg
    16201 ccgaatccag tttgatgatc tggattatcc gcggcttcgg cactgcggtg atgcacgctt
    16261 cgtgcaccgc cctgtttgcc attttgtgca agcaccttca cgaccgcaac ggccgtctgg
    16321 gcatctcctg ctggggaccc gcctggtttg ttgcagcgct catccactcg gcctttaacc
    16381 attttatcct ctccccgctc ctttccactc tggtaatcct ggcgatcctg ccggttttca
    16441 tcatcttggt ctatgaatac agcgaacgct cgctgcgcaa atggctggcc ctcagcatgg
    16501 atgaagagat ggccatgttg gagatcatcc tcagcggccg cttcacagag agcaaattcg
    16561 gacagtatct gctctccctt cgtgaccatt tcccaccggc cgttgtggtg gatctgctct
    16621 gttatctgcg catttacctc gagctggcga tgcgtgccaa ggggatcctg ctgctgcggc
    16681 agcatggcct gcctgcccag ccggataccg ctctcaggga gaaattcaat gaattcaaat
    16741 atctcgaaaa gtccatcggc cgcaccggca agctggccct ggtccccttc atgcgcatca
    16801 gggaccggga actgtggcag ctctacatgc tcgagaagta ggctgccggc gggtggaaca
    16861 ggaatcttaa gaccggcgat tcctgccgaa aagaatcccc tcattttttc ttgtaattaa
    16921 caggatgatt gggtactttt tactaatgtg gcggcaggca gggtacagca gcgacacgga
    16981 ggagcgtgac atgaaggtga tggtcgtcga tgacgaacgg gatatcgagc tgcttttccg
    17041 gcagcatttc cgcaaggaga tccgccagca gcagatcgat ttcagctttg ccctttccgg
    17101 tgaggaagcc ctgcagcagc tgcgcgacat gcgccagccc ccggcggttg tcctcatcct
    17161 ctccgatatc aatatgcccg gcatgaatgg cctgcagctg ctcaaggccg tcaagcatga
    17221 tttccccgcc atcagggtcg ccatgatcac cgcttacagt aaccagtact atgaggatgc
    17281 catggcgtcc ggagcggatc actactatac caaacccata gattttacgc agcttaaaaa
    17341 tgagctcttc atggaggcgg tccatggcta aaaaccgctt actgatcctg ctgctgctcg
    17401 gcgcaatcgc ccatgcggct gaaaccggca tgccatttat gaccgtgtat cccagcaaag
    17461 agttcggcgg ccacacccag ttctggtcga tcgaacagga taaacgcggg ctgatgtata
    17521 tcggggatgg ctatggtata caggaattcg acggcgcgaa ctggcggctg atcatcaatg
    17581 ccaaccatac ctttggcaga tctctctcca gagatgaaaa cggcaggatc tatgccggtt
    17641 cctccgctat gctgggatac ctggcagcgg acgaacgcgg agagatgcag tactgctcgc
    17701 tcatggagta catcaagccc gaagaccgcc agtttaatta tgtctggtcg gtccaggcca
    17761 cccgcgaagg tatctatttt cagaccaacg aaaggctttt ccgctttcat ccccccgaat
    17821 ccggcgccgc agccggggtg tggacggtcg atgtctggcg cccgcaagaa ttgttcggct
    17881 ataccttctg gctggatcag actctctacg tccagcagta tggcgtcggc cttatgaaaa
    17941 tggagcagga ttcgctggtg ctgctccccg gcggagggca gtttgccgat gaccggatca
    18001 atgtcatgct gcccttcccg ggcaaggctg gacattacct ggtaggcaca tttacccgcg
    18061 gattgtttct gtgggatggg accacattcc agcctctccc gaccgaggtg gacaacctgc
    18121 tccgccaggg aacgatttat gccggcgtgg ttacgccgga cagctgtttt gccctcggta
    18181 ccatggccaa tggactggtc atcatcgacc gtgatggcag gatgagactg catttttccc
    18241 agactaatgg gttcctctcc aatacggtga gctgcctgta catggaccgg caaaacaacc
    18301 tctgggtggg catggatggg ggggttgctg tgctcgaata caattctccc ctctcagagt
    18361 ttatcattcc tggcggcagc ggcccttccg atttttgccg ataccacggt gtactctata
    18421 ttacctgcaa cgatggcgtc tattatctcg atgaagtgga tggccggttc aagatgatcg
    18481 acggcattgc cggcaatgct caatctttct tttttttcca gatcaatggc gaactctttg
    18541 tcacggtcaa cgagggcatc tatcgtattc agggcaagaa gccccatttg gtcatgccgt
    18601 cccgggaaat gtccgtaccg gttctgcata tgagcgctct gagcctggcg cccgatctga
    18661 ttatcgccgg aaaatccagc ggggccgatc ttttgcgcta taacgcctcc catccccaac
    18721 gtctcagctg gttgggcagc ataccggagg tttacgaata tatccggcaa gctatggaag
    18781 ccgaaccagg cgtggtctgg ctgggtacca tggatgccgg gttgatccgg ctgactttca
    18841 ataaggaccg gctgcttgac cctgttatcg aaagatttgg catggaacat ggattgccgc
    18901 cgggcggggc ggtattctat aacccgggcg gccggccggt gttttgcaca aaaaaaggtg
    18961 tttaccagtt caacccggct caaaacagat tctcacccga cccgtttttt aaggatttgg
    19021 gcctgggtat aaatcccgat gaaagcatca ttgtggcgga tgcccagggc aacttctggg
    19081 ccaatctggg caaggaatcg gctgtatata aaaaattgcc tgatggccaa tacaaactgg
    19141 aaaaggcgca gttggcgcga ttcgtcgatg aagtgatcaa tattatcttt ccggagagcg
    19201 atggcgtcat ctggttcggc gcggcgaacc gcgtcatccg tttttcgcac ggcctgcaag
    19261 agagggatct gaccgatttt ccggcgatca ttcgccgcgt cgccctggcc ggcgattcgg
    19321 ttgtctatta cggcgctgcc ctgcccggcc atcgtctgca ggacccggcg gcacagcgtt
    19381 ttccatttcg caacaatgcc ctgcgtttcg atttttccgc ttcgtcctat cttaatcctc
    19441 gcgccaacgc tttccgcacc cggctcgagg gcttcgatgc aggctggtcg tcctggagcc
    19501 gtgaaacccg gcgcgactat accaatctcc ccgccggaaa ataccgcttt cgcgttcagg
    19561 cgcgcaacat ctttcaacag gagagcaagg aagatctcta tgccttcacc atcacccccc
    19621 cctggtacag aagttggtgg gcatggctgt tctacctgct ctgcgccggc ggcttgatcg
    19681 ctgggctggt tcgcgttcgc acccggcagc tggaggagcg cggccgggcg ctggaaaaga
    19741 tcgtccagga gcgtacggcc gagatacagg cgcagaaaaa taacgtcgaa aagctcagcc
    19801 tgatcggccg cgagatcacc gcgaccctct cgatcgggaa tatcatccac accatctatg
    19861 aaaacgtcaa tcaattgatg gatgcctccg tctttggcat cggcctctac aatgcagacc
    19921 agcagaccct cgatttcccc ggcacgaccg agaagaacga gcttctggct ccctatactc
    19981 tctcgctctc ggatgagaac cgcctggcgg tctggtgctt caagaaccag aaagaggtga
    20041 tcatcaacga ctatgccgag gatttcagcg cctatatcca gcaactgcag gcgccggtcg
    20101 ccggcgaaaa tcccgaatcc attctctacc tgcccctgac ctacaaggat aagcgcatcg
    20161 gcgtcatcac cgcgcagagt ttcaataaaa acgcctacac cgattaccat ctcaacatgc
    20221 tgcgcaacct ggccacctac agcgccattg ccctcgaaaa cgccgatgcc taccgccagg
    20281 tgaatgagct gctcgccgat ctcaagaaca cccaggaaaa actggtcaca cagtccaagt
    20341 tggccgcgct gggcgccctc accgccggca tcgcccacga gatcaagaat cccctcaact
    20401 ttgtcaacaa ttttgccgag ctcatcaccg atctggtcgc cgagctgagg gaggtcttga
    20461 cggcagaaat gggcgaggcg gcgacagcga aaaaaaacga ggccatggag atcctggata
    20521 ccctgcagca gaatggtgcc aagatcatcg agcatggcaa gcgggcggac agcattgtca
    20581 agagcatgct gcagcattcc cgcggcaaat cggatgaacg ccagcccacc gatatcaatg
    20641 ccgtgctcgc cgaagatatc aatctggcct accacggcat gcgcgcccag gacagcacct
    20701 ttaatatcaa gatcgagacc gattttgatc ctgccgtcgg caaactgaat attgtccctc
    20761 aggatgtcag ccgcgtcttt ctcaacatca tcaccaacgg ctgttacgag gcgcatcgca
    20821 aaaaacagga acaaaacggc gagttctcgc caacgctctc ggtcaaaacc cggaacctcg
    20881 cgaacctggt cgaggtgcgc atccgtgaca acggcaacgg gattccggca ggtgttcggg
    20941 aaaaactctt cacacccttt ttcaccacca agccgcccgg taagggaaca ggtctcggac
    21001 tgtcgatcag ctacgacgtc atcgtacatc agcacggcgg ccagttattc tttgaatccg
    21061 aagaaggccg atttgcagaa tttatcatcc acttgcccaa ataacgccta ttctcaggag
    21121 aaacagatgc ccgcaaaaat cctggtcgtg gatgacgaac cggatctgga acagctcatc
    21181 ctgcaaaaat tccgcaaaca gattcgcgag ggagccttca cctttaaatt cgccggcaat
    21241 ggggtccagg ccctcgaacg gctggcggag gacgaagcca tcgatctggt tctgaccgat
    21301 atcaacatgc ccgagatgga tggcctcact ttgctgaatc gcatcaagga acaggagaac
    21361 ccgcttctcc gctccgtcat cgtctccgct tacggggata ttgagaatat ccgcacggcg
    21421 atgaatcgcg gcgcctttga ttttatcatc aaacccatcg acctcaacga tctcgagatc
    21481 accattgaca aatcgctgaa cgacctgcgg gtgctcaagg aggcgatgca gtcgcgcgac
    21541 cagctgctgt tcatcaagca cgagctcggt atcgccactg agattcagac ctcgatcctg
    21601 cccaagatgt ttcccccatt ccccgatcgc aaggagttcg atatctatgc gaagatgatc
    21661 ccggcccgcg aggtcggcgg tgatttgtat gattttttta tgatcgacaa agcgcgcgtt
    21721 ggcgtcatca ttggggacgt ctcgggcaag ggcatccctg cggcgatgtt catggcggtg
    21781 agcaagaccc tgctcaaggc cacggctctg aaaggcatcc ccccggattc ctgccttgag
    21841 agtgtcaaca aggttctggt cgacgagagc ctgccctcga tgttcgtcac ggttttttac
    21901 ggcatctttg atacgcgcaa tggttcgttc gagtattgca acggcggcca taatcctccc
    21961 tacctcatca gtgcggaggg gaaggtccgt cagcttgaca atcgcggcgg ggttttcctc
    22021 ggggccttga agaatctcga atatgaatcc aatgtcgtca tgctccagcc cggggagacc
    22081 ctgtttctga ttaccgacgg catcaccgag gccatgaacc ccggggagga gatgtttggg
    22141 gaaaagcgga cagaagaatc cctgcagtcc cacgccggtg aatccctgaa caatctcgtg
    22201 gaaaacatga tcaaagaggt acaagccttt gcgggtgcgc aggagcagtc cgatgacatg
    22261 acctgcctga cgctgcgcta ctttggccgg taaaggaagt gttcgcgaag cggcgcgcca
    22321 gctgcatgca catacctggc tttctgcttg aaattttcaa tgcgatttgt tattatcagg
    22381 cagcgacagg atgaatatta agacggccta tgctgagcct gccttgattg tacggcttta
    22441 gagctcttca cagagctgga tcaccacggc atttttcgat cgcctcctgg cagcattgct
    22501 ggactttctg caggatcccg agaatccgat atggcggaaa aaaagagatc ccacatttcg
    22561 ataattttac tgggtctcct cccggccacc ctctgtagtg cgtccaacag cagccccgtt
    22621 tttgaccgcg tgaccatcaa cgatggcctc tccaataatt cgatcaatga tatcctgcag
    22681 acccatgatg gttatatctg gatcgccacc aaggatggcc tcaactgtta tgatggccag
    22741 catttcaagg tcttcaagca cgatcccatc gatacgaata cagtgcccga aaactatgtc
    22801 tattgtctgc tcgaaagcag agatagcacc ctgtgggttg gcacctgggg ggggggccaa
    22861 cccacagggt gctatctctg ctttcgagca ga
//
LOCUS       DAOPWU010000017        22840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_2257_length_22840_cov_5.212991, whole genome shotgun sequence.
ACCESSION   DAOPWU010000017 DAOPWU010000000
VERSION     DAOPWU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22840
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_22840_cov_5.212991"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="PKM23_02065"
     CDS             <1..1552
                     /locus_tag="PKM23_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNY90288.1"
                     /translation="QIRPSCRERVGDTSMRIAAKIFLLIFSLLQSAQGGRYYLSTTGS
                     DGQVGSIHAPWATFAYAMTRLRPGDSLLVREGVYREGEIWIRKAAGMGGADGRFVTLM
                     AYPGELVQLDGSRRIIVDADFMRIQGFTFCNTYSLDFPSWESPGLSTNAEILDNHFVG
                     NFLLPIEFCGRNSLIEGNTIDSRDGSSHAIYLHYGRNNIIRRNRIIGTYKYGIHMYDE
                     HKNEDPAGFVREYHNIILEDNFITGCRTRAGIVVGTSADSPTPGVIMDSVVIRKNIIT
                     ENATEGILVKAWSGRISHVDIYNNTIYQNGEAGIKIDDAHEIRIFNNIIISSSAPHID
                     VDARSGKVTAQRNLYGPGSRRLKGVEDDMPLEADPLFDSTSGTGFSLLPGSPARDTGL
                     PNNLEYSGSAPDLGAREASPTSGKPGEEPSETGWLRFMPCYPNPLNGRTVIIGHLFKP
                     FDIRVGIYDSLGRQITLLKEGPAPAGKLKLEWDGHTSSGMPAPSGVYFCCIQFGGSMV
                     TRKIALVH"
     gene            1574..4060
                     /locus_tag="PKM23_02070"
     CDS             1574..4060
                     /locus_tag="PKM23_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90289.1"
                     /translation="MLTFNLNHGLQRCCGLLQAFLLAFALSGETSSTAPNDPDQFAAP
                     LEREIRSGAKHTYSLIVPYHPRIWAWGEQEWDPQSTGTIAWRIVHGTLVNPSGAENDQ
                     SKQEFYQVAEIPDYIMGVVADQSDQEFGRRFLWTTLAGHARRYGWDQSLPGSLNPYSS
                     KTYNPQNNADQYYADARLKLLGMKNISKFYEWAYYICVYGSVAYDWLLPLKFSDGNPV
                     LSEQDKTDLQNLLFENGDLLREWADGNGHFFADRDLAGYVYFMVGLALYEPSRVDDPG
                     YKEINAKARLYLDEFDTQWVGKILPALNAQGGDGGWHGGFAFMSEFLESYYSNKTVIP
                     WQIAPLLFAHYTATGEPIERSLFSTGVVKNSVLFQNYMIRPSGYYYPPLPEEDARMPW
                     VGPMRLYARRRFSTDDEQRRIAELGAWLRAVASPSWFVNEGSWDSFDQLIFEDKWVNP
                     RSPAELGFPLSHWFRQLGWVFMRSGFTSREDLAALFVCERFRWSEMDPTSQNNFTLDY
                     MGSLIEGYHNTVLVNGEPQRSITDFPAINEGLEAFSPGSSYDIGPGVVKFSSGSDYDF
                     ICGDATHAYTANHVERAIRSIAYLKNIRTFIVYDHIITRADNAPCSWIINPGGNPELI
                     GADLMRIGNGKADLWAKRLFPEAGTIITANADKYEWRAESSAKEIEFLHVLQPSKTGL
                     ESSSPAVIADDARLSNVNGVIGCQIGEWHVLFNDTSMAVVSGPTGVKTGAVTPPAGFF
                     LNQNYPNPFNGSTSIAFSLTEAMEVDLAIYDLKGRCQITLMHGLADSGQHQIRWDGKT
                     RNGTSVSSGVYFCILRSDRRQLIRKLLLLQ"
     gene            4168..5493
                     /locus_tag="PKM23_02075"
     CDS             4168..5493
                     /locus_tag="PKM23_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90290.1"
                     /translation="MNAWFAKNLVYRGVQFIRREKVLARLEEMRTLQGMPADQLKQLQ
                     LEKLRVTLIRAGQQIPYYRELFARHDVVPEEMKLPDEMAAIPLLTKEPLQSNPDDFAG
                     ACQTGPISRETTSGTLGNPLVIAVDRNKSAMIRAVMFRNYALYGIGVGDRQARFWGIP
                     TLRRLRYREFIKDSLANRIRLSAFEITEEGLARFARKLRRFKPAYLYGYPSLLYKFSL
                     WMKEHGEKMDDLPLKAVITSGELLHDFQRTTIRQAFHCSVVNEYGTTETGIIAFECPA
                     GNLHINSDHVFLESIQSAPHETPGVLVVTELNNTYNPLIRYRLGDLGCVSDQPCACGS
                     GFPVLQELAGREGSFIKTPDRGQHFSALLSYTFTRGIKQFQAVQNSQEELCVKIVPDG
                     TLNSQMLESYKYRLAQVLGDQILITFDLVTDIEPESSGKLRYFISNIKA"
     gene            5508..6296
                     /locus_tag="PKM23_02080"
     CDS             5508..6296
                     /locus_tag="PKM23_02080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNY90291.1"
                     /translation="MSTTILMYHEVTDQQESPDSILIPSPDYCLPLNKFAEQIAYLYE
                     QGYKGISPDDLLEPNRREANECLITFDDGYIGNYLHAAPLLARYGYSGVIFVAAKWIG
                     YSERWMAWSHLRELADRGYAIESHTWSHSALEELTDKEIFFELAESKSVIENEIGKAV
                     RFISLPYGSYRENIFALAAAAGYSGIFISNAHQSISGRLPATFERIAIKEGYSLQTFR
                     DLVANDKWLMMRRRLGQETKDFIKKTIGIQRYRRLYRRAKGMKF"
     gene            6415..8268
                     /locus_tag="PKM23_02085"
     CDS             6415..8268
                     /locus_tag="PKM23_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNY90292.1"
                     /translation="MPGMIGGIGCKTEFRDALLSGFTEIWGKCDTILTEDTFLAGHAF
                     GGKAPLYGGPQGLQLGVDGEHAIYKNAARFAAGEDPSFLLISDGHVKPGVQCKGNVAV
                     LDNRNKTLHLAVEWGGSFPLYYTTLGGGVLFSSHLRPLAKALHAEADMLGWMEYLKFA
                     FIPEGRTFFKEIYRLQPGQALVYTSASGRLQLYENSQAWHDWEPPIIFKQLVEETWTI
                     YEAAIRRCLEYSRQNGLMASGGWDTRLLLAGLRAMGDKGLLCYTHGDLKCKEVALTQK
                     MVKDLGLKHHLQAIDGTMFAHENLRSGFARTEVINPLWQWAGRHLAETGVECVIAGVY
                     GEVIGGRHGRHWPFLPISEREKSRLVLSYLKYYRSNRHPEHPTDISKFYELLRLENLN
                     KPWYIRRDFWESIPQLKAEMHAAMQKFVDRLEQRGVRSVEKICEAFTAEYFGSQTLMQ
                     QHLCCREYLDIAIPFVDQELWNLTSRIPLQFKLLHSLQQALLRYKANDLLAYPNAASF
                     INSRFPLPVLEAMRVTRKLYEAIAWKISRMSRGYYAPQSIGWSNFAALGQCQALQNIT
                     DDLQCEWLDKEAIKDGVCMKIRELKTNPTMYPLNAMQNKLMAIYNADLMLN"
     gene            8355..10256
                     /locus_tag="PKM23_02090"
     CDS             8355..10256
                     /locus_tag="PKM23_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90293.1"
                     /translation="MKLAAFFSSEENGHRHIVEKMCKAQCVTDDHTYQIYTLECGAIG
                     CINTSDRYSAVPHLYIPASGNILAISGVPLRMQGGLTQMFRAISEMDCQTAANALAKF
                     DGAFAALFWDNEQKLVAIVNDFSGNQPLYQLATGQSLALATDLKGLAASGLSALEIDP
                     VGWGSFLSLGYFVGKHSSLKQVEILEPGSITRFNPRNGARTVQKYWQWREPRPHLKLS
                     QIDTGELVDQLEEHAKACLAHTPHGTLLLSGGFDSRLLLCVLRRAGISAEILIHDHPD
                     ENRNADGEYARRIANRFGLTPSTISSPDSFFISDGFIDYLVMNEITTPSLNLFIAQLL
                     SRFTGRPDGEPQDPGGSRTRPAAIQSVWEGVGPGNTLYPCIQTEGGFDNYFKFIMPAR
                     DSIAWRAARLVFAPSLVDEMYHGLTAVLAQERARYADDEFGVRNFIMESRVRNRAAAN
                     PLKVYANCVLPFTPGLSRDFVNSVNTIPYSLKINLDLYLKLFREQFPEALSVPWCSGG
                     VLIGPEGKINRIHRIYMAADKKGYIETIKRFPLIEGIARQIGRQPRKQHDHPLVGAIT
                     ARAELSHPDLNGQGIQSLGQGRRSRSIDAYARSLLFYWQVWRWLMEGQVSTLRAAAAK
                     AREEMQVVE"
     gene            10353..12047
                     /locus_tag="PKM23_02095"
     CDS             10353..12047
                     /locus_tag="PKM23_02095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY90294.1"
                     /translation="MYRIKSVTDYNEFLELEEPWNKLLAESGIDNPYLRFEWFRIAFD
                     FFEKESQLSILIVEEEAKMVAILPLVKVVEYHFGFRIKKLRFLTNVYTPFQDLIVADE
                     NSAALECILDHLKKNRSMWDLLEFKESRIQEQRYDHFIASCRKRGLFYHQFYLSRSWC
                     VATDIPWENHLERIPAKTQKEFRRKIKRAENLGSLALVPITEYAEMKRHLDLFFEFYK
                     HTWKGEELAADFYYEIAKAFSRKGHVVLYCLNIGGRPVAYMYGLCLHSILFGLKTTYD
                     PAYYAYAPGIILFYRILESLFGESRISCFDIGRGEERYKQEMASVPVHQYNILCGDRS
                     KLVPFVCHLRYALSTALKKNSSYNTLVKGFYTVKDSLSQMANQRAAAFTSSFRHSKET
                     IFYKTLDGARAGNEEEGLVCRKAEESDLDLLAVAMQSRNLKELGERLQRELCYLVWRD
                     GVLVSYFWLSREPDASGLLSLRETDLALLEFGTLAATGDGLDTRRVFEQIGDTLGQTN
                     YTTVFARLNGTVSMNEEALATLGFRKEKTVSTQRFFGLPIRRTRTFHENAEKPMLG"
     gene            12055..13293
                     /locus_tag="PKM23_02100"
     CDS             12055..13293
                     /locus_tag="PKM23_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90295.1"
                     /translation="MREEKPAVFVFGRTINALAIIRSIGSRGVRTVMVDSHPGEIAFY
                     SRFCDQKVRIHEMSEDPELVLHELIDAAGKQDQRPVLLCTRDEYLVFLSKYQTILQDH
                     FIFSLPEPDMVSTIIDKRLLAQFALQRGIPHPRAYAVHTGEEAAILSQDLCYPVIIKP
                     PASHKAGQQQKWMGQKLLIAHDHFELLNYLEEFGKDQIDILIQEQIPGPETDIFACYA
                     CFSRDSRPLAMMTLRKLRQYPIHFGYGCAEQSDWVPEVAELGVKVFTEMKYRGMGGVE
                     FKCDARDGIYKMIEIDGRAPMNVGIAIASGVDLPWIAYCDLAGEDCIPTDSFKKGVKW
                     FFFEDDLAAYLQYRSRNEITLKEWVKTYRGRKSFATLSCTDPLPFLVSITRFLKGIVH
                     SNWHRIRRKLSLQNQHFIDY"
     gene            13339..13875
                     /locus_tag="PKM23_02105"
     CDS             13339..13875
                     /locus_tag="PKM23_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90296.1"
                     /translation="MLDCQLHSLEEAAEALPWCDRLKRTLQRWGINMFRPLYHLLKKL
                     RFGKGFHILRRRKMSERALPGPVMAPLNLQPGERVRVKSFKEIQGTLNEDNKFQGLAY
                     TPAMKKYCGGTYTVVKRVERAFDERRWKMAAIRNVVLLDGVFCDGVGGPTREWDGCDR
                     CCYIWWKEGWLERVADEK"
     gene            13865..14977
                     /locus_tag="PKM23_02110"
     CDS             13865..14977
                     /locus_tag="PKM23_02110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNY90297.1"
                     /translation="MKNDHVILLAAGDIMVSSNRGTGAAIDKYGASFPFMKVQPVLAG
                     ADLRFANLESPISAHGRVSGKQDAHITFRASPEAAEGLAYAGFDIVSLANNHMNDYGD
                     EALLDTLALLQDKGIDHVGAGRDEEEACRAVIVKRKGIRIGFLAYSAFANLDTRPACG
                     DKSGIAWFIKRRAMQKIRHLRSEVDIVIVSLHWGLDFAEYPVPFQMKQARALVDAGAH
                     LVLGHHSHLLQGTEQYRHGIIVYSLGDFVFDEEGRETCLLRVELSRSGIEKIDFIPAR
                     INDKFQPEPLSIVDGQKIRDRIAALSAAYTGYDSAAAQKMVDFYIRTNWQIFRGSGNR
                     FALRNLLQIGIILRMITLFFMKVKKFYSGQSTDAAL"
     gene            complement(15068..16084)
                     /locus_tag="PKM23_02115"
     CDS             complement(15068..16084)
                     /locus_tag="PKM23_02115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNY90298.1"
                     /translation="MKSIKNHLKHALVSTGLLRLYGEHRPLRISVLFYHGFRKTGLSG
                     QAGGKDNFSPVELFERHLQVILKYAHPVSILDLLGERKIPRHAVIITLDDGYANNYTL
                     AFPLLRKYGLPATIFLTTGFIDRQTFLWTDWLEWVITRADANKSLPAWGETKVPLRLE
                     SEIDRKQTMLNFKSYFKTLPISAIHSSLLELQDHLNCRYAWSDLPPSCQPLTWSQIRE
                     MAACGLITFGSHTVSHPVLSRCSAEVQRQELQISKNRIEEETGEPCVLFAYPNGTAED
                     YSVQTVEQLREIGYKLAVTTDPGYERADRFDPLRIRRWGADISATDLAFILAGGADRH
                     GRGD"
     gene            complement(16400..18121)
                     /locus_tag="PKM23_02120"
     CDS             complement(16400..18121)
                     /locus_tag="PKM23_02120"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNY90299.1"
                     /translation="MFCGLAASARAQLSTPLLEWRDDVGDTTTRSTVHLLARTAWPAL
                     VTINGDSCHLYRTGIFFKSLTLQPGSNLITATVKPVVGEEARFTRTIFRREVSPRAAL
                     PLWLDSTSVLPAESTLLQSRDRLRVSFIGAKGHAGWVDFKPGKLRLYLRRTDLADCSR
                     YEAELPLSRLPMDKEHTLQLCLKNTIKGAKKQTLRREAGVTIRVQSAERFPLLQVIRE
                     GALLNQSLGPVRLGPPVISELPAGTILQSSGRIGSSWRIRLDDESEGYIEAGEVEPLA
                     EPALRPAYYITTLGAAPAAGVDLVTIPRLQPVPFSLHPDPAGRILRLRLYGVQSSSTW
                     ITHREGLRVMDRLSWEQIAPESYQVNIHLQDTRIWGYTLTAGPTMLELRLKHPPAGTI
                     KLAIEAGHGGSNLGAVGLSGLLEKMVNLDVAQRLEKLCREAGLQVLQVRDADIDMSLG
                     AKKERVEVSEADLLVSIHANAGGTANGYLGANGVSTYYNNPFWGDFARVVYRSLLELP
                     LQEFGCVGSFNYRVIRMSSRPAILVELAFLSHAGDEEKLFLPEFRQQLAEKILAGVTK
                     FAASMRE"
     gene            18382..19218
                     /locus_tag="PKM23_02125"
     CDS             18382..19218
                     /locus_tag="PKM23_02125"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNY90300.1"
                     /translation="MPKKPLATHFSFPFQNNLIYNNPEKRRIARDMLERYTGSDAADF
                     QQNIILTNFHKYLDVFQERSGTKITSGSAFSAVHNPGENISMIDFKIGAPTAAMVIEM
                     LSAIETQAVLFLGMCGGLHRSLKVGDFILPMAAIRDEGVSGHFIPPQVPSLPTFKIQK
                     FVSQIIVEHNLDYRTGVVHTTDYRFWEFDDTFKAILYEERAIGIDMECAALFSVGFVS
                     KVPIGALLLVSDLPLQKDGIKTKESSRKVFSTFTAKHIEIGISSMIEIQKRGEHIRHY
                     RW"
     gene            19278..20255
                     /locus_tag="PKM23_02130"
     CDS             19278..20255
                     /locus_tag="PKM23_02130"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNY90301.1"
                     /translation="MTMQTRFMNLQLKNPIIVASSGLTRSADKIRACEEAGAAAVVMK
                     SLFEESLAREEWNLEAGAVGHAEAYDYMRAELGKQYGPREYCSMLETVKKSVKIPVIA
                     SINCISAKWWPSYARQFESAGADALELNVHVTVADARTTAGEAEKLYFDILDAVKAQV
                     KIPVAMKIGRHFSSLPHLAAQLGERGLDGLVLFNRFTQFDIDIHSRRLGMTFDFSRPE
                     EHHEVLRWVALLSGQTRCHLAATTGIHSGQEVIKMLLAGARAVEIASAFYKKGVGLIP
                     TLLAEIEAWMKEQGQNDLESCIGSLNMAGSSAPELYLRAQFMEKIRGWE"
     gene            20501..21283
                     /locus_tag="PKM23_02135"
     CDS             20501..21283
                     /locus_tag="PKM23_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90302.1"
                     /translation="MAAYLYLSIFPESLIASMLPPEEFGVYFATGTAKRSRGQALFLE
                     IDRDFTSDYFNLDDIAQRCQPHIDGQPKRSVYLSIYRVLEHLPVSVLKNLYLVSDEGK
                     VLELPKSSHLPEEQRQLHLYQELCPVMPRIATLFNPLEFCRFVTNRQFEVSVPRLFFM
                     EMELGGLAFDPLLAPADELPYAHIEHLRDCLIGLAQQPEKPTKTVSRYLRSDISFATI
                     KNGFFIGDQQEICYYPFPAHEVLLRDHYEWWQSVRDTEDLEL"
     gene            21344..>22840
                     /locus_tag="PKM23_02140"
     CDS             21344..>22840
                     /locus_tag="PKM23_02140"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNY90303.1"
                     /translation="MVEQLFWLAPIGSAAALIFAYIFYAQMKKASEGSERMAQIASYV
                     RTGAMAYLRQQYKVVTMVFIGLVILLSFMAFVLKVQNGWVPVAFLTGGFFSGLAGFFG
                     MKTATYASARTAEAARKSLDGGLRVAFRSGAVMGLVVVGLALLDISLWFLILRHVYPI
                     EGGDGHNLVLITTTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKVEANIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLYESYAGSILATAALGAAAFVTDSSMQLKAVIAP
                     MVIAAIGTLLSIAGIFVVRTKEGASQKQLLSALGRGVNFSSILIVMASYFVVHALGLG
                     TGVFWAIVCGLLAGIIIGKATEYFTSAGFKPTQRIADSAKTGPATVIIAGIGTGMMST
                     TVPVLSVVVGTVLAYLTAAGFDFSNVNMGLYGIGIAAVGMLSTLGITLATDAYGPIAD
                     NAGGNAEMTHQAPEVRKRTDALDSLGNTTAAVGKGFAIGSAALTALALL"
BASE COUNT         5109 a         6237 c         6389 g         5105 t
ORIGIN      
        1 gcagatccgg cccagctgcc gggaaagagt cggtgataca agcatgcgca ttgcagccaa
       61 aatatttctg ctcatcttct cgcttctgca atccgcccag gggggacgct actacctttc
      121 gaccacgggg tcggatggtc aagttggctc catccatgct ccctgggcca cctttgccta
      181 tgcgatgaca agattgcgcc caggtgattc cctcctcgta cgggaaggag tatatcggga
      241 aggtgagatc tggattcgca aagcggccgg aatgggggga gcggatggcc gttttgtcac
      301 cctcatggct tatccgggcg aactggtgca gcttgatgga tcgcgccgta taatcgtgga
      361 tgctgatttt atgcggattc agggtttcac cttttgcaat acctacagtc tggattttcc
      421 ctcctgggag agccctggac tgtccaccaa cgccgagatt cttgacaacc attttgttgg
      481 caattttttg ctgccaatag aattctgcgg caggaacagt ctgattgaag gcaataccat
      541 cgacagccgg gacggatctt cacacgctat ttatctgcat tatggccgca acaacattat
      601 tcgcagaaac cggattatcg ggacctataa atacggaatt cacatgtatg atgaacacaa
      661 gaacgaagat ccggccggct ttgtccgcga atatcacaac attattcttg aagataattt
      721 catcaccggc tgccgcacgc gcgccggtat cgtagtggga acttcggctg atagtccgac
      781 acccggcgtc atcatggaca gtgtcgttat ccgcaagaac atcatcacag aaaatgccac
      841 agagggcata ctcgtgaagg cctggagcgg ccggatcagc catgtcgata tttataataa
      901 caccatctac caaaatggcg aggcgggcat caagatcgat gatgctcacg agatccgtat
      961 ttttaacaac atcatcatct cctccagtgc tccccacatc gatgtcgacg cgagatccgg
     1021 aaaagttacc gcacagagaa atctttatgg acccggctca aggcgtctaa agggtgtgga
     1081 ggatgacatg cccctggagg ccgacccgct gttcgatagt accagtggga caggtttttc
     1141 gcttcttccg ggcagtccgg cacgggacac cggattgccc aacaacctcg aatacagcgg
     1201 ctcggccccc gacctggggg caagagaggc atcgccaacc tccggcaaac caggagaaga
     1261 accgagcgag acgggctggc tgcgcttcat gccctgttat cccaatcctt tgaacggcag
     1321 aacggtgatc atcggccatc tttttaaacc gtttgacatc cgtgtgggta tctatgactc
     1381 gctcggccgg cagataaccc ttctgaaaga gggccctgct cccgccggga agctgaagct
     1441 ggaatgggat ggccatacca gctccggtat gcctgcgccg agtggggtct acttttgctg
     1501 cattcaattc gggggctcca tggtcacccg gaaaatagcc ttggtgcatt aaaaaaatta
     1561 tgggcggtgt gaaatgctga ctttcaacct taaccatggc ctgcagcgct gttgcggcct
     1621 gctgcaggca ttcctgctgg ccttcgctct gtcgggtgag acctcatcga ccgcaccaaa
     1681 cgatccggac cagtttgccg cacccctgga aagggagata cgctcgggcg caaaacatac
     1741 ctacagcctc atcgtaccct atcacccgcg catctgggca tggggggagc aggaatggga
     1801 tccccagtct acgggcacta ttgcatggcg aattgtgcat ggcacacttg tcaacccgag
     1861 tggagcggag aatgaccaat ccaagcagga attttatcag gttgcggaga tcccggatta
     1921 tattatgggc gttgttgcgg atcaaagtga tcaggaattc ggtagaaggt ttctctggac
     1981 cactttggcc ggtcatgccc gccgttatgg ctgggatcaa agtctgccag gctccctcaa
     2041 tccctattcg agcaagacct acaatcctca gaacaacgct gaccagtact acgccgatgc
     2101 gcgcctgaag cttctcggca tgaaaaatat cagcaagttc tacgaatggg cctattacat
     2161 ctgcgtctat ggctcggttg cctatgactg gctgctgccg cttaaattct cagatgggaa
     2221 tcctgttttg tctgaacagg ataagacgga cctgcaaaat ctgctgtttg aaaatgggga
     2281 tttgctgcgg gagtgggctg atggaaatgg gcattttttt gccgaccgag atctggccgg
     2341 gtacgtctac tttatggtcg gtctggccct ctacgaaccc tcccgcgtgg atgacccggg
     2401 ctataaggag atcaatgcaa aagccagact ttatctcgat gaattcgata cccaatgggt
     2461 gggaaaaata ttacccgccc tcaatgcgca gggaggagat ggcggctggc atggtggatt
     2521 tgcttttatg agcgagtttc tggaatcgta ctattcgaac aaaacggtca tcccctggca
     2581 aattgcccct cttctttttg cccattacac tgcaacaggt gagccgattg aacgttcgct
     2641 tttcagcacc ggtgtagtga aaaacagtgt tctatttcaa aattacatga tcagacccag
     2701 cggttattat tatcctccgc ttccggaaga ggacgcgcgc atgccttggg tcggcccgat
     2761 gcgcctttat gcccgccgcc ggttctccac cgatgatgag cagcgcagga tcgccgagct
     2821 gggcgcctgg ctgcgcgcgg tcgccagccc gagctggttt gtcaacgagg gctcctggga
     2881 ctcttttgac cagctcatct ttgaagacaa gtgggtcaat ccccgctctc ccgccgaatt
     2941 gggattcccg ctcagtcact ggtttcgtca attgggctgg gtgttcatgc gcagtggttt
     3001 tacctcaagg gaggatctgg ccgccctctt tgtgtgcgag cgcttccgct ggagtgagat
     3061 ggatccgacc tcgcagaaca actttactct ggactatatg ggctccctca ttgaaggtta
     3121 tcataacacc gtgctggtca atggtgagcc tcagcgctcc atcacggatt ttcctgccat
     3181 caatgagggg ctggaggcct tttccccggg aagtagttat gatatcggcc cgggtgtagt
     3241 caaattctcc agtggcagtg actacgattt tatctgcggg gatgcaacgc acgcctatac
     3301 agccaatcat gtggaaagag cgatcaggag tattgcttat ctaaaaaata tccggacctt
     3361 tattgtttac gaccacatca tcaccagggc tgacaacgcg ccctgcagct ggatcatcaa
     3421 tcctggagga aatccggagt tgatcggggc tgatctgatg cgcatcggca acgggaaagc
     3481 cgatctctgg gccaaacgtc ttttccccga ggcgggtaca atcatcacgg ccaacgccga
     3541 caagtacgaa tggcgcgcag agagcagcgc aaaagagata gagtttctcc atgtcctgca
     3601 gcccagcaaa accggattgg agagcagttc acctgcggtc atcgccgatg acgcccggct
     3661 ttccaacgtc aatggcgtga tcggctgcca gataggggag tggcatgttc tttttaatga
     3721 caccagcatg gccgtagtct ccggaccgac tggagtgaag accggagctg tgacgccgcc
     3781 ggccggtttt tttttgaacc agaactatcc caatcccttt aatgggtcca cctccatcgc
     3841 tttttcgctg actgaggcca tggaggtcga tctggcaatt tacgacctca agggcagatg
     3901 tcagatcaca ttgatgcatg gtctggccga ttccggtcaa catcaaatcc gttgggatgg
     3961 caaaacccgg aatggaacat ccgtcagttc cggagtctac ttttgtattt tgcgcagcga
     4021 tcgccgccag ctcatccgca agctgcttct gctgcaataa ggacgccggt tgttcctgca
     4081 ccacatcgcg gcgttacagc cacccctgat aattcagtgc gtgcttgcag ttttcgtgca
     4141 gagaaataaa caccacacga aaccaagatg aatgcatggt ttgccaaaaa cctggtctat
     4201 cgcggcgtcc agttcatacg ccgtgaaaaa gtactcgctc gccttgagga gatgcgtacc
     4261 cttcaaggta tgccggccga ccagctcaag caattgcagc ttgaaaaact gcgcgtgacg
     4321 ttaatccggg ctggccagca gattccgtat tatcgcgaac tttttgcccg acacgatgtg
     4381 gtgccggaag aaatgaagct gcctgatgag atggctgcta ttccactgct gaccaaagag
     4441 ccgttgcaga gcaatccgga tgattttgca ggtgcctgtc aaacgggtcc gataagccgg
     4501 gagacgacca gcggcaccct gggcaatcct ctggtgatcg ctgtcgatcg taataaaagc
     4561 gctatgatca gggcggtcat gttcagaaac tatgcccttt atggcatcgg tgtcggggac
     4621 cggcaggccc gattctgggg aattcccacc ctgcggaggc ttcgctaccg cgaatttatc
     4681 aaggatagcc tggccaatcg aatcaggttg tcggcgtttg agatcacgga agaaggcctg
     4741 gcacggtttg cccgaaaact gcgccgtttc aagccggcct atctgtatgg ctatccttcc
     4801 ctgctgtata aattttccct ctggatgaaa gaacatgggg aaaaaatgga cgatctgcct
     4861 ttgaaggccg tcatcaccag cggggaactg ctgcacgatt ttcaaagaac aaccatccgc
     4921 caggcttttc actgcagtgt cgttaatgag tatggaacga ccgagaccgg cattattgcg
     4981 tttgaatgtc cggccggaaa cctgcacatc aacagtgatc atgtctttct ggaaagtatt
     5041 caatcagctc cccatgaaac ccccggcgtc ctggtcgtca cggagctgaa caacacctac
     5101 aatccgctca tccgctacag gttgggggat ctcggctgcg tctccgacca gccctgtgcg
     5161 tgcggcagcg gattcccggt tctgcaggaa ttggccggca gggaaggcag ctttatcaaa
     5221 acccctgacc gggggcagca tttttccgca ctgctatcct atacctttac ccggggaatc
     5281 aagcagttcc aggctgtcca gaacagccag gaagagctct gtgtgaaaat agtgcccgac
     5341 ggtaccctga acagccagat gctcgaaagt tataaatatc ggcttgcgca ggtccttggg
     5401 gatcagatcc tcatcacttt tgatcttgtc actgatatcg aaccggaaag ctccggaaaa
     5461 ctgcgctatt tcatctccaa tatcaaagcc tgaagagatc gaggcctatg tccaccacca
     5521 ttctcatgta tcatgaggtg actgatcagc aggagagtcc ggattcgatt ttaattccat
     5581 cacctgatta ctgcctgcct ctgaacaagt tcgcggagca gatcgcctat ctctacgagc
     5641 agggctataa ggggatctca cccgacgatc ttctggagcc caaccgaaga gaagccaatg
     5701 aatgtttgat cacctttgat gatggctata ttggcaatta cctccatgcc gctcccttac
     5761 ttgcccggta tggctattcg ggcgtcatct ttgttgcggc caaatggatc gggtacagcg
     5821 agcgctggat ggcgtggagt cacctgcggg agctggcgga caggggttat gccatagagt
     5881 cccatacctg gtctcattcc gcactggagg agttgacgga caaagagatc tttttcgaac
     5941 tggcggaatc gaaaagtgtg attgaaaatg agatcggcaa ggcggttcgt ttcatcagcc
     6001 tgccttacgg cagctaccgg gaaaacattt ttgcacttgc tgcagccgca ggctactccg
     6061 gcatattcat ttcaaacgcg catcaaagca tatccggccg tctgccggcc acattcgagc
     6121 gtatagcaat caaggagggg tattcgctgc agaccttccg ggatctggtt gccaatgata
     6181 aatggctgat gatgcgcagg cgtttggggc aggaaaccaa ggactttatc aaaaagacca
     6241 tcggaatcca gcgctatcgc cgcctgtacc gccgcgccaa ggggatgaaa ttctgagccg
     6301 ctgggaaaag gctcttccgc ctcacccgga tccttgcgca gggaaggttc gaaaatttta
     6361 tcaggcatat ggtgcacagt tgtttcgctg caggatatgg attaggaggg cgtcatgccg
     6421 ggaatgatcg gagggattgg ctgcaaaacc gagttccggg atgctttgct gagcggtttt
     6481 accgaaatct ggggcaaatg cgatacaatt ctgaccgagg acacttttct tgccggacat
     6541 gcgtttggcg gcaaggcgcc gctgtatggc gggccgcagg ggttgcagct cggggtcgat
     6601 ggtgaacacg cgatttataa aaatgccgcc cggtttgcgg cgggtgaaga tccctccttt
     6661 ctgctcatct ccgacgggca tgtcaagccc ggggtgcagt gcaagggcaa tgtcgctgtt
     6721 ctcgataatc ggaacaaaac tctccacctg gcagtggagt ggggggggag tttcccgctg
     6781 tattatacga ctctgggagg cggagtgctt ttcagcagcc acctgcggcc gctggcaaag
     6841 gccctccacg cggaggccga tatgctcggc tggatggaat atctgaaatt tgcattcatt
     6901 ccggaaggtc ggactttttt taaggaaata taccggctcc agccgggtca agcactggtt
     6961 tacacctccg catccggccg gctgcaactc tacgaaaata gccaggcctg gcacgattgg
     7021 gagcccccca tcattttcaa acagctggtg gaagagacct ggacgatcta tgaggctgca
     7081 atccgtcgct gccttgagta tagccggcaa aatggcttga tggcgtccgg gggatgggac
     7141 acacgactct tattggcagg attaagggcc atgggagata aaggactcct ttgctacact
     7201 cacggtgatc tgaaatgcaa agaggtcgcc ttgacccaga aaatggtcaa ggatctgggg
     7261 cttaagcacc atctgcaagc tatcgatggc accatgtttg cgcacgaaaa tctgcggagc
     7321 ggttttgccc gaacagaggt tatcaatccg ctctggcagt gggccggaag gcacctggct
     7381 gaaacagggg tcgaatgtgt gattgctggc gtatatgggg aggtaatcgg cgggcgtcat
     7441 ggccggcatt ggccctttct gcccatcagt gagcgggaga agagcaggct ggtcttgtca
     7501 tacctaaagt actaccgaag caacagacac ccagagcatc caaccgatat cagcaagttc
     7561 tatgaactgc tgcggctgga aaatttaaac aagccctggt atatccgccg ggatttctgg
     7621 gaatcgatcc ctcaactcaa ggcggagatg catgcggcca tgcaaaaatt tgtcgaccgg
     7681 cttgaacaac ggggcgtgag gtccgtcgaa aaaatatgcg aggccttcac ggctgaatat
     7741 tttggcagcc aaaccctgat gcagcagcac ttgtgctgcc gcgagtacct ggacattgcg
     7801 attcccttcg tggaccagga attgtggaac ctcacgagca ggatccctct gcagttcaaa
     7861 ttgctccatt cactgcagca ggcgttgctg cgatataaag ccaacgacct tctggcctat
     7921 cctaatgcgg catcgttcat taattccaga ttcccgcttc cggtgctcga agccatgcgg
     7981 gtgaccagaa agctctatga agccatcgcc tggaaaataa gcagaatgag tcggggatac
     8041 tatgcgccgc aatcgatcgg atggtccaat tttgcagccc tggggcagtg tcaggccttg
     8101 cagaatatca ctgatgatct ccagtgtgaa tggctggaca aggaggcgat caaggatggc
     8161 gtctgcatga aaatcaggga gttgaagacg aatccaacca tgtatcccct caatgccatg
     8221 caaaataagc tgatggccat ctataatgca gatctgatgt tgaattagcc gatccatgcc
     8281 aacacacaga cttgtccgca gatgaaccgc agacaggccg aaacggaaat atacttcttg
     8341 caggagtttg ccagatgaaa ctggctgctt ttttcagttc agaagaaaat ggtcatcgcc
     8401 acatcgtcga aaagatgtgc aaagcccaat gtgtcacgga tgaccatacc tatcagatct
     8461 ataccctgga gtgcggggca atcggctgca tcaatacctc ggacaggtat tcagctgtgc
     8521 cccatctcta cataccggcc tcaggcaata ttctggcgat cagcggtgta ccgctgcgca
     8581 tgcaaggggg ccttactcaa atgttccgtg ctatcagtga aatggactgc caaacggctg
     8641 caaatgcact ggccaaattt gacggcgcct tcgcagcgct tttttgggat aatgaacaga
     8701 aactagtggc cattgtcaat gatttctccg gcaaccagcc gctctaccaa ttggccaccg
     8761 gccagagcct ggcactggcg accgatctca agggactggc agcaagcggc ctctctgctc
     8821 tggagatcga cccggtcggc tggggttctt tcctctcgtt gggctatttt gtcggcaagc
     8881 acagttctct gaaacaagtc gaaatcctcg agccgggcag tatcacccga ttcaatccgc
     8941 gaaacggtgc ccgcaccgtt caaaagtact ggcagtggcg cgaaccccgt ccacatctga
     9001 aactgtcaca gatcgacacc ggagagctcg tggatcaatt ggaggagcat gccaaagcct
     9061 gcctggccca taccccgcat gggactttgc tgctgagcgg cggttttgat tcgcggctgc
     9121 tcctatgcgt tctgcgcagg gccgggatct ccgcagagat tctgatccat gatcatcccg
     9181 atgaaaacag gaatgcggac ggtgagtacg cacggcgaat cgcaaaccga ttcggcctga
     9241 ccccgagcac catttcatcc cccgactcct ttttcatatc cgatggcttt atcgactatc
     9301 ttgtcatgaa cgagatcacc actcccagcc tgaatctctt tattgcacag cttctttccc
     9361 gttttaccgg ccgcccggac ggtgagccgc aagatcccgg aggttcgcgt accagaccgg
     9421 cagcgattca atccgtttgg gaaggggtgg gacccggcaa cacgctttac ccctgcatcc
     9481 aaaccgaagg tggattcgat aattacttca aatttattat gccggcgcgg gacagcatag
     9541 cctggcgtgc tgccaggctg gtttttgcac cttcgcttgt ggatgaaatg tatcatgggt
     9601 tgaccgccgt tctggctcaa gaaagggcca ggtacgcgga cgatgaattc ggtgtgcgaa
     9661 attttattat ggagagccgg gtcagaaacc gggctgccgc aaatccttta aaggtgtatg
     9721 caaattgtgt tttgcctttc actcccggat tgagcaggga tttcgtcaat tctgtgaaca
     9781 ctattcccta ttccctgaaa atcaatctgg atctctatct gaagctcttt cgtgaacaat
     9841 ttcccgaggc cttatcggtt ccgtggtgct caggaggcgt gctcatcggg ccggaaggca
     9901 agatcaaccg aatccaccgc atttatatgg ccgcggataa aaaggggtat atcgaaacca
     9961 tcaagcgatt tccgctcatc gaaggtattg cacgccagat agggcggcag cctcgcaagc
    10021 agcatgacca tcccctggtc ggagccatta cggcccgagc ggagctcagt catcctgatt
    10081 tgaacgggca aggtatccaa tcgctcggac aagggcgtcg gtcgcgttca atcgatgcgt
    10141 atgcacgttc cctgctcttt tactggcagg tttggcgttg gctcatggaa gggcaggtat
    10201 caaccctcag agcggccgca gcgaaggcca gggaagagat gcaagtggtt gagtaggaat
    10261 ggtttttccc atgccgggaa agcactcaag gggaaaagag cgcctctggc agatatggac
    10321 ggaggcgacg gcatgaaagg aaggcaatgc tgatgtatcg catcaaaagt gtaactgatt
    10381 ataatgaatt cctggaactt gaagagccct ggaacaaact gcttgccgaa agcgggatcg
    10441 acaatcccta tctcaggttt gagtggttca ggatcgcctt cgatttcttt gaaaaagaga
    10501 gccaactcag catcctgatt gttgaagaag aagccaaaat ggtcgccatt ctcccgctgg
    10561 tgaaggtcgt cgaataccat ttcggattcc ggatcaaaaa attgcggttt ttgacgaatg
    10621 tctatactcc ctttcaggat ctcatcgtgg cggatgagaa cagcgccgcc ctggagtgca
    10681 tccttgatca tctgaagaaa aaccggtcga tgtgggacct tctggaattc aaggagagcc
    10741 gcattcagga gcagcgttat gaccacttta tcgcttcgtg ccgcaagagg ggactctttt
    10801 atcaccagtt ctacctttcc cgaagctggt gcgttgcgac ggatattccc tgggagaacc
    10861 atctggagcg catccctgcc aaaacgcaaa aagagttcag aaggaaaatt aaaagagctg
    10921 aaaatctggg ttctcttgct ctggtgccga ttacagaata cgcggaaatg aaaaggcatt
    10981 tggatctttt tttcgaattt tacaagcata cgtggaaagg ggaagaactt gctgcggatt
    11041 tctattatga aattgcgaag gctttcagca gaaagggcca cgtcgttctc tactgtctga
    11101 acatcggcgg gcggccggtc gcctatatgt acggcctgtg tttgcattct atcctcttcg
    11161 ggctcaagac cacctacgac ccggcctact atgcttatgc cccggggatt atactttttt
    11221 acaggatcct cgagtccctc tttggggaat cccggatcag ctgttttgat atcggccgcg
    11281 gcgaggaacg ctacaagcag gagatggcct ctgtgcctgt ccatcagtat aatatccttt
    11341 gtggcgatcg ctcaaaactg gtgccttttg tctgccatct tcggtatgcc ctctccacgg
    11401 cgctgaaaaa aaacagctct tataataccc ttgtaaaagg gttttataca gtcaaggact
    11461 cgctttccca aatggcaaac caaagggcgg ccgcctttac ttcctctttc cgtcacagca
    11521 aggaaacgat tttttataaa acactggatg gcgcgcgtgc tggcaatgag gaggagggtc
    11581 tggtttgcag gaaagcggag gaatccgatc tcgatctgct cgcagtggcc atgcaatcca
    11641 gaaacctgaa agagcttggg gaaaggctgc agcgtgagct atgttatttg gtctggcggg
    11701 acggggtttt agtcagttat ttctggctgt caagagagcc tgatgcatcc ggcttgctgt
    11761 cactgaggga gaccgatctg gccctgctcg agtttggcac cctggctgcg acaggggatg
    11821 gccttgacac aaggcgtgtt tttgaacaga tcggcgatac cctggggcaa accaattata
    11881 ccactgtttt cgccaggctg aatggcacag tatccatgaa cgaagaagca ctcgcaaccc
    11941 tgggattcag aaaagaaaaa acggtctcca cacagcgctt tttcggcctc ccgatcaggc
    12001 gtacgcgcac gttccacgag aatgcagaaa aacccatgct tggatgacga gccgatgaga
    12061 gaagagaaac cggcagtttt tgtctttggc agaacgatca atgccctggc cattatcagg
    12121 agcattggca gcaggggggt ccgcactgtt atggtcgact cacatcccgg cgagatcgcg
    12181 ttttactcgc ggttttgtga ccaaaaggtg cgaatccatg aaatgagcga agatcctgaa
    12241 cttgtgctcc acgagctgat tgatgcagcc ggcaagcagg atcaacgccc tgttctgctt
    12301 tgtacacggg atgaatatct tgtttttctc tcaaaatatc agaccatact ccaggatcat
    12361 ttcattttca gtctgcccga accggatatg gtcagcacca tcattgacaa acggctgctg
    12421 gcgcaatttg ccctgcagag ggggatcccc catcccaggg cctacgctgt ccacacaggc
    12481 gaagaggcgg cgatcctcag tcaggacctt tgttatccag tcatcatcaa gcccccggca
    12541 tcgcataaag cagggcaaca gcagaaatgg atgggccaga aactgctcat cgctcatgat
    12601 catttcgaat tgctgaatta tctggaggag tttggcaaag atcagattga tatcctgatt
    12661 caggagcaga tcccggggcc agaaacggat atttttgcct gctatgcatg cttttcccgg
    12721 gactcccggc ctctggcgat gatgacattg cgcaagttga ggcaatatcc catccatttc
    12781 ggctatggtt gtgcggagca aagtgactgg gtgcccgagg ttgcagaact gggcgtaaag
    12841 gttttcactg agatgaaata tagaggtatg ggaggcgttg aattcaagtg tgatgcacgg
    12901 gatgggatct ataaaatgat cgagatcgac ggccgtgcac ccatgaatgt cggtatcgcc
    12961 atcgccagcg gcgtcgacct gccctggatt gcttattgtg atcttgccgg tgaggactgc
    13021 attccaacgg attcgttcaa gaaaggagtc aagtggttct tcttcgagga tgatcttgcg
    13081 gcctatttac aatatcgcag ccgcaatgag atcactctca aggagtgggt caaaacgtac
    13141 agggggcgga aatcatttgc gaccttgagc tgtacggatc ccctgccttt tctggtgagc
    13201 attacacgtt tcctgaaggg gatcgtacat tccaactggc atagaatcag gcgcaaactg
    13261 agtctgcaga atcagcattt tatcgactat taaataatgg attaacactt catcccacta
    13321 ctgattcggt gaggccccat gttggattgt caattacact ctctggaaga agctgccgaa
    13381 gccctgccgt ggtgtgacag gctcaagaga actctgcagc gctgggggat caacatgttc
    13441 agacccctct accatctcct gaaaaaactt cgttttggaa aagggttcca tattttgcgg
    13501 cgcaggaaga tgagtgaaag agctctcccg ggcccggtga tggccccgtt aaacctgcaa
    13561 cccggcgagc gagtgcgggt aaaatccttc aaggagattc agggcactct caatgaggac
    13621 aacaaattcc aggggctcgc ttatacgcca gccatgaaaa agtattgcgg aggaacctat
    13681 acggttgtta aaagagtgga acgggccttc gatgaacgca gatggaaaat ggcagcaatc
    13741 aggaatgtcg ttctcctgga tggagtcttt tgcgacggag tcggcggacc caccagagaa
    13801 tgggacggct gcgatcgttg ctgctacatc tggtggaaag agggctggct ggagagagta
    13861 gccgatgaaa aatgatcatg tgatcctgtt agcggcagga gacatcatgg tctccagtaa
    13921 tcgcggaacc ggggcggcta tcgacaagta cggagccagt ttccccttca tgaaggtcca
    13981 gccagtgctt gccggtgccg atctccgctt cgcgaatctt gaaagcccga tatcggccca
    14041 tggccgggtt tccggtaaac aggatgcgca catcactttt agagcatcac ccgaggccgc
    14101 tgaggggctg gcctatgctg gttttgatat tgtctccctc gcgaacaacc acatgaatga
    14161 ctatggggac gaggctctac tggatacgct ggccctcctt caggataagg gcatagacca
    14221 cgttggagca ggccgcgacg aggaagaggc ctgccgggcg gtgattgtca aacgcaaagg
    14281 aatcaggatc ggatttctcg cctattccgc ttttgcaaat cttgataccc gcccggcctg
    14341 tggtgataag agtggcatcg cctggttcat taaacgcaga gccatgcaaa agatcagaca
    14401 cttgcgctcc gaagtggata tagtaattgt ctcgctgcat tgggggctgg atttcgcaga
    14461 atatcccgtt cccttccaga tgaaacaagc gcgggcgctg gtcgacgccg gggcacatct
    14521 cgtcctgggg catcattccc atcttctgca gggaaccgag cagtaccgcc atgggataat
    14581 cgtctacagc ctcggagatt ttgtttttga cgaagagggg agagagacat gcctgctccg
    14641 ggttgagcta tccaggagcg gtattgaaaa aattgatttt attcccgcga gaatcaacga
    14701 taaattccag ccggagcctc tctcaatcgt ggatggccaa aaaataaggg acaggattgc
    14761 tgctctgtcg gctgcctata ccggttacga ttctgctgcg gcacaaaaga tggttgactt
    14821 ttacatcaga accaactggc aaattttcag ggggtcgggc aaccgcttcg cgctgcgcaa
    14881 tctgctgcaa ataggcataa tcctgaggat gattacactc ttcttcatga aggtgaaaaa
    14941 gttctattcc ggccagtcaa cggatgcagc gctctgattt cacaaggcgg agacagtgcg
    15001 aggaccggta ttatcgactg agcgggacca tcggctgcac cgtgattcca tcattgtaga
    15061 gcccacttca atcgcctctc ccatggcggt cggccccgcc tgcaaggatg aaggccagat
    15121 ccgtcgcgga gatgtctgcg ccccaccgcc gtattcggag cggatcgaac cggtcagccc
    15181 gttcatagcc cggatccgtc gtcaccgcaa gcttatagcc gatctcccgc agttgttcaa
    15241 cggtttgtac tgaataatcc tctgccgttc cattcgggta ggcaaacagc acgcagggtt
    15301 cgcctgtttc ctcctcaatt ctatttttgg agatctgcag ctcctgacgc tgcacttcag
    15361 cactgcacct cgaaaggaca ggatgggaaa cggtgtggct tccaaaggtg atcaggccgc
    15421 atgcagccat ttcgcgtatc tgcgaccagg tcaagggctg gcatgacggc ggcagatcac
    15481 tccaggcata gcggcagttc aagtggtcct gcagttccag cagactggag tgaatggcgg
    15541 atataggcag ggttttgaaa taggatttaa aatttagcat ggtttgcttg cggtcgattt
    15601 ccgactccag ccgcaggggc accttggttt ctccccatgc cggcagagat ttattggcgt
    15661 cagctcgggt gatcacccac tccagccaat cggtccataa aaaggtttgc cggtcaataa
    15721 acccggttgt gagaaagata gtcgccggca gaccatattt ccgcagcagc ggaaaagcca
    15781 aagtatagtt attggcatag ccgtcgtcaa gtgtaatgat tactgcatga cgggggattt
    15841 ttctctctcc gagaaggtcg aggattgaaa ccggatgggc atatttcagg atgacttgca
    15901 gatggcgttc gaacagttca acaggcgaga agttgtcttt tccgccggct tggccggata
    15961 atcctgtttt cctgaagcca tgatagaata acacgctgat acgaagcggg cgatgttcac
    16021 catacaaccg cagcagcccc gtactgacaa gtgcatgttt aagatgattc tttatactct
    16081 tcaacggaca aaatcctcgt gagccgctcc tgcaaatcaa aaaggtccag ctgtttaatg
    16141 gcctggcagg cagtccctga tagtcatttc aagacggtcg aacggatgat gtgttgtacc
    16201 ctcccggggt gataacttgt tgaacagcct ctaacttaat gatacgtttt ccacgttagg
    16261 aatacaagta aaaagagatt cctttctggc gagaaaaaca aaattatgaa tagtccgaac
    16321 gcgcgaccgg gaaagctcga tttgtagccg catgctgctg atgaccggca gctgtgagta
    16381 gatcgcaagg acggatgtgt tactctctca tggatgcggc gaattttgtt actcccgcca
    16441 gaatcttctc ggccagctgc tggcggaatt cgggcaggaa gagcttctcc tcgtccccgg
    16501 catgggagag gaaggccagc tcgaccagga tggccgggcg tgaactcatg cggatgaccc
    16561 ggtagttgaa ggagccgacg cagccgaact cctgcagggg cagttcgaga agactccggt
    16621 agaccacgcg ggcgaaatca ccccagaagg gattgttgta gtaggtgctg acgccgttgg
    16681 cgccgagata tccgttggct gttccccccg cattggcatg gatgctgacc agcaggtcgg
    16741 cctccgaaac ctcgaccctc tcctttttgg cccccagact catatcaata tccgcatcgc
    16801 gcacctgcag cacctgcagg cccgcctcgc ggcagagttt ttcaagccgc tgtgcgacat
    16861 cgaggttgac catcttctcc aaaagtccgg agaggccgac cgcgccgaga ttggagccgc
    16921 cgtggccggc ctcgatcgcc agcttgatgg tgccagcggg ggggtgcttg aggcgcagct
    16981 ccagcatggt gggcccggcg gtgagggtgt agccccaaat ccgggtatcc tgcaggtgga
    17041 tgttgacctg atagctctcg ggggcgatct gctcccagct caggcggtcc atcacgcgca
    17101 gcccctcacg atgagtgatc caggtgctcg aactctgcac cccgtatagg cggaggcgca
    17161 ggatgcggcc ggccggatcg gggtggaggg agaaggggac gggctggagg cgcgggatag
    17221 tgaccagatc gacgccggcc gcgggcgcgg cgcccagggt ggtgatatag taggccggcc
    17281 gaagcgccgg ctcggccagg ggttccacct ccccggcttc gatatagccc tcgctctcgt
    17341 catcgagtcg gatccgccag gaggagccga tgcgcccgct gctctgcagg atggtcccgg
    17401 ccgggagttc gctgatcacc ggcggaccga ggcggacggg ccccaggctc tggttgagca
    17461 gcgccccctc gcggatgacc tggagcaggg gaaagcgctc cgcgctctgc acccgaatgg
    17521 tcaccccggc ctcccgccgc agggtctgct ttttcgcccc cttgatggtg ttcttgaggc
    17581 agagctgaag ggtgtgctcc ttgtccatgg gcaggcggga gaggggcagc tccgcttcgt
    17641 agcgcgaaca gtcggccagg tcggtgcggc gcagatagag gcgcagcttg cccggtttga
    17701 aatcgaccca gccggcgtgg cctttggcgc cgatgaagct gacgcgcagc cggtcgcggc
    17761 tctgcagcag cgtggattca gcgggcagga ccgaggtcga gtcgagccag agcggcaggg
    17821 ccgcgcgcgg actgacctcg cggcggaaga tggtgcgggt gaagcgggcc tcctcgccga
    17881 cgacgggttt gaccgtcgcg gtgatcagat ttgaaccggg ctgcagggtt aaactcttga
    17941 aaaagatgcc tgtgcggtag agatggcagg agtcgccgtt gatggtcaca agcgccggcc
    18001 aggcggtccg ggcgaggagg tggacggtgc tccgggtggt ggtgtccccg acatcgtccc
    18061 gccactccag gagtggagtg ctgagctggg cgcgggcgct ggctgccagc ccgcagaaca
    18121 aaataatgag gagccggagc cgactggtgt atttatgcat aagactgttt cccatccgtg
    18181 tgcaagagga ggaaccgccg ggtattggga taatttagaa acccggccgc aaaaaccaag
    18241 caaaaaaact caggtgggga gcagatttca gggtgcccgg caagatttca ggtcgggccc
    18301 ggattttggg gctgccctgc cggcaactaa cgcttgcttt ttatccacaa ctatttgtat
    18361 ctttatggaa aaggagcgaa aatgccgaag aagccactcg caacccactt cagcttcccc
    18421 tttcagaaca atctgatcta taacaacccc gagaagcgca ggatcgcccg ggatatgctc
    18481 gagcgttata ccggctccga cgccgccgat tttcagcaga atatcatcct caccaatttt
    18541 cacaaatatc tcgatgtctt tcaggaacgc tccggcacga aaatcacctc cggctctgcc
    18601 ttttcggcgg tgcacaatcc cggcgaaaat atcagcatga tcgatttcaa gatcggggcg
    18661 ccgaccgcgg ccatggtgat cgagatgctc agcgccatcg agacccaggc agtactcttt
    18721 ctcggcatgt gcggcggact gcaccgctcg ctcaaggtgg gcgatttcat cctgcccatg
    18781 gccgccatcc gtgatgaggg agtcagcggc catttcatcc cgccgcaggt gccttctctg
    18841 ccgaccttca agatccagaa attcgtcagc cagattatcg tcgaacacaa tctcgactat
    18901 cgcaccgggg tggtccacac caccgattat cgtttttggg agttcgacga cacctttaag
    18961 gcgatcctct atgaagagcg ggcgatcggc atcgacatgg agtgcgccgc cctcttcagc
    19021 gtcggctttg tcagcaaggt gccgatcggg gccctgctgc tggtctccga tctcccattg
    19081 caaaaagacg gcatcaagac caaggagtcc tcgcgcaagg ttttttcaac cttcaccgcc
    19141 aaacacatcg agatcggcat ttcctcgatg atcgaaatcc agaaacgcgg cgagcacatt
    19201 cgccactatc gctggtgacc ccggccaggg tccgcaccgg cgcatacgac caatccagca
    19261 ataaggagtg gaacgagatg acaatgcaaa cccgcttcat gaatcttcag ctcaagaatc
    19321 cgatcatcgt cgccagcagc gggctgacca ggtcggcgga caagatccgc gcctgcgagg
    19381 aggccggggc tgcagcagtg gtgatgaaaa gcctcttcga ggaatccctc gcccgcgagg
    19441 agtggaatct ggaagccggg gccgtcggac atgcagaggc ctatgactat atgcgcgcgg
    19501 agttgggcaa gcagtacggc ccgcgggagt actgttccat gctggaaacg gtaaaaaagt
    19561 cggtcaagat tccggtgatt gccagcatca actgcatctc ggccaaatgg tggccgagct
    19621 atgcccggca attcgagtcc gccggcgccg acgccctcga gctcaatgtc catgtcactg
    19681 ttgctgacgc ccgcaccacg gcaggcgagg ccgaaaagct ctattttgat atcctcgatg
    19741 cagtcaaggc ccaggtcaag attcccgtgg cgatgaaaat cggccgtcac ttctcctcgc
    19801 tcccccacct cgcggcgcag ttgggcgaga ggggccttga cgggctcgtc ctcttcaacc
    19861 gcttcaccca gttcgacatc gacattcata gccgccgcct gggcatgacc ttcgatttca
    19921 gccgtcccga ggaacaccac gaggtgctgc gctgggtggc cctgctctcc ggccagaccc
    19981 gctgccacct ggcggcaacc accggcatcc acagcgggca ggaggtcatc aagatgctcc
    20041 tcgccggagc gagggcggtg gagatcgcct cggcttttta caaaaagggg gtgggcctga
    20101 tcccgaccct gcttgccgaa atcgaggcct ggatgaagga acagggccag aacgacctcg
    20161 agtcctgcat cggctccctc aatatggccg ggagcagcgc tcctgagctc tatctgcgcg
    20221 cccagttcat ggaaaagatc cgcggctggg agtaatccgc tcctgccgct gggcatggtg
    20281 tctcgtgcgc tcacggccgc gaggagatca tccaggcggc ctgctctccc cctcaagcat
    20341 tcaccctgcc cgtccgaagg gcgggcaggg tgaatgccac acctccagcc agctgttttc
    20401 cgtttccttc tttgtacagc cattcagatg ttgacaatga gcaataatta ttgtaaattt
    20461 acggctatat ttccattact tgcgctgaaa ggaaggggtg ttggccgcct atctctatct
    20521 ctccatcttt ccggaatccc tgatcgcctc catgcttccc ccggaggagt tcggggtcta
    20581 tttcgccacc ggcaccgcca aacggtcgcg cggccaggcc ctctttctgg aaattgacag
    20641 ggattttacc agcgactatt ttaaccttga tgatatagcg cagcgctgcc agccgcacat
    20701 cgacggtcag cccaaacgtt cggtctatct ctccatctac cgcgtcctcg agcatctccc
    20761 cgtctccgtc ctgaaaaatc tctacctggt ctccgacgag ggcaaggtgc tcgagctgcc
    20821 caagagttcg cacctcccgg aggagcagcg tcaactgcac ctttatcagg agctttgtcc
    20881 ggtgatgccc cgtatcgcca ccctcttcaa ccccctcgag ttctgccgtt ttgtcaccaa
    20941 tcgtcaattc gaggtctcgg tgccccgcct ttttttcatg gagatggagc tgggcggact
    21001 ggccttcgat cccctgctcg ccccggccga cgagctgccc tacgcccata tcgagcatct
    21061 ccgcgactgt ctcatcggcc tggcgcagca gccggaaaag ccgaccaaga cggtctcgcg
    21121 ttatctgcgc agcgacatca gcttcgccac catcaagaac ggttttttta tcggggatca
    21181 gcaggagatc tgttattacc ccttccccgc gcacgaggtg ctgctccgcg accattatga
    21241 gtggtggcag tcggtgcgcg ataccgagga tctggaactt taattaaccc ttgcagcaac
    21301 gggctcgggg agtccggtac atcctaaaca gtggagcgac acaatggttg aacaactctt
    21361 ctggctggcg ccgatcggat cggcggccgc cctcattttc gcctatattt tttatgcgca
    21421 gatgaagaag gccagtgaag gcagcgaacg catggcgcag atcgcctcgt atgtgcgcac
    21481 gggcgccatg gcctatctga gacagcagta caaggtggtg acgatggtct tcatcggcct
    21541 ggtcattctg ctctccttca tggcctttgt gctcaaggtg cagaacggct gggtgccggt
    21601 ggcttttctt actggcggtt ttttctcagg tcttgccggc tttttcggca tgaagaccgc
    21661 cacctacgcc tcggcgcgca cggctgaagc cgcacgcaag tccctggacg gcggcctgcg
    21721 cgtcgccttc cgcagcggtg cggtgatggg gctggtggtc gtcggtctgg ccctgctcga
    21781 catttcgctc tggtttctca tcctgcgcca tgtctatccc atcgagggtg gtgacggcca
    21841 taatctggtc ctgatcacca ccaccatgct gactttcggc atgggcgcct cgacccaggc
    21901 cctcttcgcc cgcgtcggcg gcggcatcta caccaaggcg gcggatgtcg gggccgacct
    21961 ggtcggcaag gtcgaagcca acatccctga ggatgatccg cgcaatccgg ccaccattgc
    22021 cgacaatgtc ggcgacaatg tcggcgatgt tgctggcatg ggcgctgatc tctatgaatc
    22081 ctatgcgggt tcgatcctgg cgacggctgc cctcggcgcg gccgcttttg tcaccgattc
    22141 ctcgatgcag ctcaaggcgg tcatcgcccc gatggtgatc gctgccatcg gcaccctgct
    22201 ctccatcgcc ggcatttttg tcgtgcgcac caaggaaggc gcttcccaga agcagctgct
    22261 cagtgcgttg ggacgcgggg tcaactttag ctcgatcctg attgtgatgg cctcctactt
    22321 cgtcgtccac gcactcggcc tcggcaccgg ggtcttctgg gcgatcgtct gcggcctcct
    22381 cgctggcatc atcatcggca aggcgaccga atatttcacc tcggccggct tcaagcccac
    22441 ccagcgcatc gccgactcgg ccaagaccgg gccggctacg gtgatcatcg ccggcatcgg
    22501 cacgggcatg atgtccacca ccgtgcccgt cctctccgtg gtcgtcggca ctgttctggc
    22561 ctacctgacc gccgccggtt tcgactttag caacgtcaac atggggctgt acggcatcgg
    22621 catcgccgcg gtcggcatgc tctccaccct cggcatcacc ctggcgaccg acgcctacgg
    22681 cccgatcgcc gacaacgccg gcggcaacgc cgagatgacc caccaggcgc cggaagtccg
    22741 caagcgcacc gacgccctgg acagcctggg caacaccacc gcggccgtgg gcaagggctt
    22801 cgccatcggc tcggccgccc ttacggcgct ggccctgctg
//
LOCUS       DAOPWU010000018        22353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_2323_length_22353_cov_5.814602, whole genome shotgun sequence.
ACCESSION   DAOPWU010000018 DAOPWU010000000
VERSION     DAOPWU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22353
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2323_length_22353_cov_5.814602"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /gene="recF"
                     /locus_tag="PKM23_02145"
     CDS             complement(<1..650)
                     /gene="recF"
                     /locus_tag="PKM23_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNY90304.1"
                     /translation="MYLSSLAIDSFRNLDTQRLDFYARKNYLFGENAQGKTNLLEAIY
                     LLCLCKSFRTSSDAELIPLGKDCFSLTGRFADHNGIEHEIVLRYQASSGKQVFLDGKP
                     VYQLSSLVGQFPVVVLSAADYDITHGSPQQRRRFFNILASQCSARFLNDLKEYEKIIK
                     QRNRIFWLIQNGRIKGGEELEVWNEQLIIKGCALMEFRSKLISEMAAVLAHHYQSIS"
     gene            complement(665..1825)
                     /gene="dnaN"
                     /locus_tag="PKM23_02150"
     CDS             complement(665..1825)
                     /gene="dnaN"
                     /locus_tag="PKM23_02150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNY90305.1"
                     /translation="MNFTVSQKEFFDALQKVIGVVPPKTTISILTCILLDLRSGKLSL
                     TGTDLEISITTTLDVNSSEEGSVAIPAKILVEIVRELPDVPLNIFSDADNKVTIKTEK
                     GEYKLSTQPKEDFPQISVEEGELSFQMDSGLLGRMAEQTMFAVSADELRPALTGINME
                     LLPGEIRFVATDGHRLAKITVSNGMIADDVRRNLIIPTKTLHLLLRNFEDDANVGLQI
                     GEDHIVFTLDRAVVYSKLISGTYPNYERVLPKDNELRLQINRELLISSLRRVSIFSSS
                     LTHQVRLMVAAEEVTVRSEDIEFGAEAKEVIPASFNGEWLQIGYNSNYLMDVLRHLDG
                     EEVVFELKDATSAAILKPVERREGEEVLMLLMPIRINEASQAPASESSYSAE"
     gene            complement(2159..3511)
                     /gene="dnaA"
                     /locus_tag="PKM23_02155"
     CDS             complement(2159..3511)
                     /gene="dnaA"
                     /locus_tag="PKM23_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNY90306.1"
                     /translation="MYQNHHEVWDKVLAILKDEVHPNTLLTWFKPIQPIKLDDKALTI
                     QVPNQFSYEWIEQQYGAMMSRALSQVMGNGFKLAYSIRMEAPLPADLIPPTPTKSPLP
                     VASTHADNLNVRYCFDSYVEGECNKFAKAAALAVAKSPGTTTFNPLVVYGGVGLGKTH
                     LIQAIGNYAKEHNQSIRVLYVDSERFTADFINSIQKNKTTEFSGLYRNVDILMIDDIQ
                     FFGNKERTQDEFFHTFNALHQKGKQIILSSDRPPKELQGMEERLKSRFQWGLVVDIQA
                     PDLETRQAILQKKAEESSIDLPPEIFHFIATHITSNIRELEGAMIRLLAFASLNGIDI
                     TLEVAKKVLRDVCISKTKAISIEYIQKVTAQHFSFPDDLLRSKTRKKEIAQARQICMY
                     LCKSLTDSSLKTIGLHFGGRDHSTVIHAINCTEELMETDGKYRENVEAIKNQIEISQL
                     "
     gene            4010..4153
                     /gene="rpmH"
                     /locus_tag="PKM23_02160"
     CDS             4010..4153
                     /gene="rpmH"
                     /locus_tag="PKM23_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNY90307.1"
                     /translation="MQPTFQPSKRKRVNKHGFRARMATKNGRTVLAKRRAKGRKKLSV
                     SDE"
     gene            4181..4522
                     /gene="rnpA"
                     /locus_tag="PKM23_02165"
     CDS             4181..4522
                     /gene="rnpA"
                     /locus_tag="PKM23_02165"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNY90308.1"
                     /translation="MDKSSLIKKSAEFSSIIRRGSVYKGSYFTLFVVKEDKLRIGFSV
                     KRGMKSVDRNRLKRRTRELWRAWRGEFDLSGNLIVITREQALSDPFNLLEQDFRRLLE
                     QISLHENQTSS"
     gene            4573..4794
                     /gene="yidD"
                     /locus_tag="PKM23_02170"
     CDS             4573..4794
                     /gene="yidD"
                     /locus_tag="PKM23_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNY90309.1"
                     /translation="MKINPAKELVLLLIRFYKKAISPLLPPSCRYHPTCSTYALQAIE
                     KYGLLKGGGKAMIRVLKCNPFFPGGNDPV"
     gene            4804..6540
                     /gene="yidC"
                     /locus_tag="PKM23_02175"
     CDS             4804..6540
                     /gene="yidC"
                     /locus_tag="PKM23_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNY90310.1"
                     /translation="MNLDKKTILAFLLIGLVFIFVQSPIYQKLFFPEAYKKQLEARQA
                     RPAAMDSLRGTQEQAATDHEEIAATTADSSRSRVEAEALIPERLITIESDLYTAQFST
                     RGGTLVSWTLKEYKKSENEPVSMLPSGSKGGLALDFYTRDEDTLHTAGYHFNCEAGDS
                     ILLSEGDATLRFTRQFDSDQRISKTFTFKNGRYDFDLEVVLENMGSLVADKSYQIVIP
                     NGLESTEKRLGEDMYYAKAAIAAAGRVNKIGKTRGILEKESGSIDWVAVRTKYFAYAV
                     MPQTNKAEYATIIGRELPKSGYTKEKWKKFAVTIGMPLTRENHRDRFTIFIGPMKDEL
                     LKSYNLGLEKLMDMGAKIIQPFSVAILWTFKKLHSIIPNYGIVLIIFALLIKIITAPL
                     TQKSTKSMKRMQALAPRMNELKEKYAKDPQRLNEETMKIYKEEGINPMGGCLPILVQM
                     PLLWALYIVFRSTISLRQEGFFWWIKDLSGPDTIFTLPFTIPMYGNTVNVLPILMGLT
                     MILQQKMSITDPKQKAMVYIMPIFMTLLFNSFPSGLNLYYTLFNIISIVHQKYFMGLE
                     EPAPVVVKSKKK"
     gene            6610..8013
                     /gene="mnmE"
                     /locus_tag="PKM23_02180"
     CDS             6610..8013
                     /gene="mnmE"
                     /locus_tag="PKM23_02180"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNY90311.1"
                     /translation="MSPYSENDTIAALATAHGPAALAIIRVSGPDALQICAALFTGRR
                     RINLKRADRYHGMLVEPEVGCGEAAEVYDEVILHTYPAPHSFTGEDMAEINCHGGLLV
                     VERLLGLLLRRGARLAAPGEFSKRAFLNGKLDLLQAEAVADIIAAPSRRALALSLAQL
                     HGDISLKINKLSDYLRTSCALLELELDFSEGEVLADRAQLEEKLNEAAGELRTILAGY
                     HYGQLLREGVGVVLAGKVNVGKSTLMNRLLRRDRAIVSHLPGTTRDTLEEGLRLGGYL
                     FHLTDTAGIRAAADEIEREGVARTLAAISSADLLLLIFDASTPLDDEDREIIELGRGR
                     PAGSTLYLFNKCDLLPGPEKFTPPPIASVECLLVLSVRTGTGVEELERRMIARVERER
                     PEGEGVTITRLRHHDALRRAEYHLEQARQSLSEERSGEFIALDLRMALAAAGELTGEV
                     RSADILEDIFSTFCIGK"
     gene            8065..10008
                     /gene="mnmG"
                     /locus_tag="PKM23_02185"
     CDS             8065..10008
                     /gene="mnmG"
                     /locus_tag="PKM23_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNY90312.1"
                     /translation="MKGSGDGYDILVVGGGHAGCEAALAAARMGASTLLITQNIFTLA
                     QMSCNPAIGGLAKGHLVKEIDALGGAMGVVADETALQFRMLNRSKGPAVWSPRTQNDR
                     YHYPLRMRRLLEEQENLSLRQQSVTALLIEKDEIRGVMTEAGHSVRAGAVILCAGTFL
                     NGLIHIGLNHFPGGRSGEPAAGGIAEQLAHYGLHSGRLKTGTPPRLDGRSVDLQRMEE
                     QPGDAEIIPFSFRHQNLKMDQLSCYLTRTGASTHDILRSGLDRSPLYQGVIKSIGPRY
                     CPSIEDKIHRFADKESHQLFLEPEGRESHEYYLNGFATSLPEDIQIAAVRSVPGLERA
                     HLTRLGYAIEYDYFHPTQLKANLETKSVQGLFFAGQINGTSGYEEAAAQGLMAGINAV
                     LARRGQEPFILPRQESYIGVLIDDLTTKELSEPYRMFTSLAENRLHLRQDNADLRLME
                     YGRRLGLIDDAAFTALERLREAVQAARVRLEKVNPDPERINPLLIRAGKAPLLHRESA
                     ARLLKRPELRLADLAGCGEDPLLVEELDPFWRRVGEQVEIEIKYAGFLQRQQEHIRRM
                     AGLEETLIPEEIDYEAVPSLSRESREKLARIRPRTLGQASRILGVGPEDVAVIMIHLA
                     RLRRSRSVPRGTHLRQEENGPGE"
     gene            10019..10648
                     /gene="rsmG"
                     /locus_tag="PKM23_02190"
     CDS             10019..10648
                     /gene="rsmG"
                     /locus_tag="PKM23_02190"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNY90313.1"
                     /translation="MALANDVHLREQQWEEIARYVELVESWNQRTNLVSRQDIGRIVS
                     RHVRESLWFIRPEVLGEFRLVLDLGSGAGFPGVVMKIAEPGLLLTLLDSRQIKARFLA
                     EVINHLGWDDVQAFCERAENLPGRFPGLTFDLVVCRAVAKLSDLWRWAAPLLGPGGRL
                     AALKGGDLAEELHQFGKRHPAVRWQLIPMPELPDDPAAGRQMVIVHTRI"
     gene            10667..11584
                     /gene="murQ"
                     /locus_tag="PKM23_02195"
     CDS             10667..11584
                     /gene="murQ"
                     /locus_tag="PKM23_02195"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNY90314.1"
                     /translation="MSKTVFDEIKNLTTEARNPATRHIDQQSISEVLHLINDEDLKIA
                     AVVRSEIRYIEKAVRIVVQAFKEGGRLIYVGAGTSGRLGVLDASECPPTFGVSPELVQ
                     GIIAGGYKALISAQEGAEDRYEMGAQDILARNITPRDVVCGIAASRRTPYVIGAIDKA
                     REVGCKTLYVTCNPRKEMNIKVDVAICAVVGPEVVMGSTRMKAGTATKLILNMITTTA
                     MIQLGKVWENMMVDLQMTSKKLEERSKKIVMQATGVDYESAEKVLKKADGHVKTALVM
                     ISAGVTAEEAHALLKKGEGFVHRAIALHR"
     gene            11663..15031
                     /gene="mfd"
                     /locus_tag="PKM23_02200"
     CDS             11663..15031
                     /gene="mfd"
                     /locus_tag="PKM23_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNY90315.1"
                     /translation="MSVIDTIADIFARSEAVARLEKAISTGQSIVLRGCQGAARPFLA
                     QHLRQHAHASFLCLFPGADEAKHFYDDLVELLPPELLALFTPHGKQMWNEVGPLGTVV
                     GQRLNTLNILLRREPALIVTSAPALLEKVADPVRVAGAALRLERGMNYAFPRLIEELV
                     HIGYTREMRVERPGEMAVRGGLIDLFLFEETHPMRIEFWGDQIESMRYFDIESQRSLT
                     PLESIVLLPLSAGGLYGPAQDPPFDQLPLTSLLTDYLPQDVKLLLFDNILISNELENF
                     EKEYSVRFDTFLQDHPSEGGHFSAFYSDRKSILEIINTIQTIELNTFSTQSSLPEIEL
                     STLRNHHFAGNLKPFFQEVEAHHEEARQQGITPMVAVLCDSQAQTGRLRDLLAREEMG
                     DIAVETLNLSEGFQWPGEHLFLYTDRELYGRIRLRKQDKASRRMVSFADLLKLQEGDY
                     VVHIEYGVGIFQGLKQIEAYGRTRECIQIEYANGDKLYVPLEKMDQVQKYSSRDGYVP
                     VLNKLGGKEWDKLKSRTKARVKQVAEQLIKLYAVRKLRAGHPFSPDSIWQKELEASFV
                     YDETLDQLNAVNEIKVDMEKPVPMDRLVCGDVGFGKTEVAVRAAFKALTDGKQVAVLV
                     PTTVLAQQHFATFSERLAKFPVRIGVLSRFKNPRQLAELIQQVGRGEIDLIIGTHRLL
                     SQDIRFKDLGLLIIDEEQKFGVLHKERLKMLKANVDTLTLSATPIPRTLHMALMGARD
                     MSLINTPPGNRLPIVTEVCRFERDHVREVILREVERKGQIFFIHNRVATISGIYQLLS
                     ELVPEVTFAVAHGQMEGPELEKVMTSFAEGRIQCLVCTMIIESGIDVPNANTLIVNRA
                     DRFGLAQLYQLRGRVGRSDHQAYAYFLIPPLRKLTRTAIKRLQTIQECSHLGSGYKIA
                     MRDLEIRGAGNIFGAEQSGFVDALGYELYAKIIEEAIKELREELNLAPQPKVEDDVQV
                     AIESRVEMTGEAFLPPDYVEAASDRVDIYKRLIEAKAMPAIDDLEREVLDRFGPLPEA
                     ARNLLDYVRIKMLAHLSKVEEIGCKDGKINGKFFAAAVPKGEQFRPWIGKIVQKACYP
                     FELRQIQNSLHFEFKTEAGLAPLAQIKKFLESII"
     gene            15083..15925
                     /locus_tag="PKM23_02205"
     CDS             15083..15925
                     /locus_tag="PKM23_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNY90316.1"
                     /translation="MRTGSALAASLLLFSLTCLMCTKGEEAGIVARVGQSVLTLQEVR
                     ERNPGAGTGAARSQAERYIQHWIESELLYQEALLQGVDKQPEVQRALKELSRDYIISA
                     FSEQAANAAVSVSDEEIQRYYQEQKEEFLAEADLYHLLLILVQNQNEALQLRRELIGG
                     TPFSEIASRNSLDGSRLHGGDLGYVPLSALSPMLARAVSAMQPGSLSAPVKSELGYNL
                     LRLVDVQRKGATRALEEVQPVIKQRITARKKDEALQNLISRLSSEAALYTDMTKLESL
                     EGKQ"
     gene            15928..17256
                     /locus_tag="PKM23_02210"
     CDS             15928..17256
                     /locus_tag="PKM23_02210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF020872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNY90317.1"
                     /translation="MNFAKRVSRIARGAAAGVLFLLALLLNPLQAQQVLDRILAVVDD
                     DVILESEVAQGAYLTAMQYRIDPVKSPREFARLKQETLTNLINQKILLVQADKDTVKA
                     DEQQVEHYLQQQMQSVIQQLGGEQQVEEYFGASLAKVRRNYREEIERNLRIRAVQSAK
                     MQGIKISRREVEEFFKVYKDSIGPVKESFDLSHILIKALPGEAARQAARDKAEALRQR
                     LLAGEDFATLARENSEDPGSAARGGDLGFMSRGEFVRDFEEAAFKLEAGAISEVVGSQ
                     FGFHIIQMVERRGEKINTRHILITSKPSREDEVAAADTIKAIAAQLKQGADFEQLVAR
                     YSQDENSKEDKGHLGFFEIDQLRERAKELVYATTGLKAGEYSDPVRTQYGFHILRVNR
                     REEARELDFKKDYDRIQRLALDYKTGLAFNAWIEELKKDVHVEVKDDLSN"
     gene            17339..18013
                     /locus_tag="PKM23_02215"
     CDS             17339..18013
                     /locus_tag="PKM23_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNY90318.1"
                     /translation="MTKRGRWWLLLLGAALILPIERLSADASEFTIARLKYGGGGDWY
                     NDPSSVPNLLAFLRTRSSLATAKEEVHVSLMDDQLFACPFLFMTGHGRIAFTPQEAAR
                     LRIYLTSGGFLFADDDYGMDEHFRREIAKVLPDHELLEVPFSHPIFRSPFSFPEGLPK
                     THEHDGGVPQGFAIFHEGRMVVFYAYNCNISDGWADPEVHHDPPEVREQALQMGMNIV
                     VYALTH"
     gene            18049..21390
                     /locus_tag="PKM23_02220"
     CDS             18049..21390
                     /locus_tag="PKM23_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90319.1"
                     /translation="MKRLSAFREAEKRLRVGAALSRWATHALLLLLVLVLLQSLLSLP
                     PAGRYLLLAFALLLLLASWLKHVLRPLTDLLFRPEHPSLNSIALRIGREYPQLQDRLA
                     NALQISARPEANDVKVSPELIEAVRAGIPAFLADIDLSRHLDRAPACRWLRRLAAAAA
                     LFLFAWLLFPKTLLIGLERTIHPRRGLSGQLAPEIRVEPGDRMVVRGAPVTIRAWTET
                     LMVGDLQIAIERFGRRELLSMTRGMSDTFRYAIPALRDSLLYQILYQKSATRKYLLRV
                     VDLPMIRSLQLSLQPPSYSGAEPFLLEENIGDVSALKGTRIEWRAEANKPVTEASLRF
                     SSGRILPLEVKGRQLATTFALQGEESYYAELKDNSGLASENPIVYHLRLLADQYPFVR
                     LLAPSGDVDLHEDMRLSLLIQAQDDYGLSGLAIGYQVVEENQAKIDSSRFRRLELPLH
                     RSGQAIINLAYDWDLSSSPLLPTEALLYYIEVRDNDTVSGPKSARTGLYRARFPSLYE
                     LYKEVNSEQDDAIAGLEENYEKGLELKKKLDEMAQQLKRADELSWQKKQEVGDLLKQK
                     EELKQELEKIAGNLDEMIEKMEENQLLSPETLDKYQELQELYREIMTPELQKNLEKVA
                     EAMQKVDPAQLQKALEEFKLTEEALNKSLDRTLSLLKRLRMEQQLDQAIHMNEDLQKR
                     QRELTEKTMQSQGEEREKLAKEQEGLREDLHRMEEAMADLQQEMGREPGMPEESVAAA
                     REELAKAGLQGQMEAMQGAIAQGNSDRMQSGSQQVQAGLERSREQLQQAKDAMTGAMM
                     ERAMRALQRGTRSLLSLSQMQEELLRSSAGMPQGSARVPETAERQQQLSSSLERVIND
                     LYAASKESFGITPRIGKALGQAQQAMQQALQGLENRDLGQAGQRQGQAMAGLNEAVLQ
                     MDAAMQSMAQGQGGGMSMGQFMQQMQQLADGQQGINAQTLSLSGGGESLSLAQQAAMS
                     RLAQQQGGIRKSMQQLASEAAGLSDLLGDLEHIGRDMEKVEKDLAGQRVDRSTIERQN
                     RILSRMLDYQRSMRERERSQERKAETGKEYHPSSPGELPGDLGQRRDRLQQDLLRAKE
                     EGYSRDYLELIRKYFESLGEREEKR"
     gene            21374..>22353
                     /locus_tag="PKM23_02225"
     CDS             21374..>22353
                     /locus_tag="PKM23_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90320.1"
                     /translation="MKRSVRYLLIIAVLLAALSRTAAQEKRPGDQELRILQQRAQQLE
                     AAGRLDQAGELYSRVARILPPPQNLNAYAGARRCFERSKDLAGWEALIHDLQTRQRAL
                     PFQVDLAEIAYLRGDRAEALRTWRAVIDDNPQEEQAYKLTGAVLLKYYLYDEAESLYL
                     RGRKAFKDPQRFFFELVHLYQDQGAFARMTEEYLNYLQKNAGQLGYVQAQLLSAGDEP
                     EAVRQIAGSIEREMKSSADFRLPGCQLLGSLYTQSRDYRLALQCYEELEEAARRKNPA
                     EVGQYYYTFAMAAMSDGALSEARRALEALVQQAGPRSPHRVHAAFALARLL"
BASE COUNT         4797 a         6623 c         6467 g         4466 t
ORIGIN      
        1 gagatgctct gataatggtg cgccaggacc gcagccattt cggagatcag cttgctgcgg
       61 aactccatca gcgcacagcc cttgatgatc aactgttcat tccacacttc cagctcttcc
      121 ccacccttga tgcggccgtt ctggataagc cagaatatcc ggttgcgctg tttgattatc
      181 ttttcatact ctttgagatc attcagaaat cgggcggagc actgggacgc cagaatattg
      241 aaaaagcgcc ggcgctgctg cggggaacca tgggtgatgt cataatcggc agccgacaga
      301 accacgacag gaaactgccc caccaggctg gagagctgat ataccggctt gccatcaagg
      361 aaaacctgtt tgcctgatga agcctgatat cgcaaaacaa tctcgtgctc gatcccgtta
      421 tggtccgcaa acctcccggt gaggctgaaa caatctttcc ccaggggaat cagttctgca
      481 tcgctgctgg tgcggaatga tttacacagg cagagtagat agatggcttc gagtagattg
      541 gtcttgccct gtgcattttc gccgaagaga tagtttttcc tggcataaaa atcgagacgc
      601 tgtgtatcca gatttcgaaa agagtcaatc gccaaactgc tcagatacat agccggtccg
      661 gaggttactc ggcgctgtag gaagattcgg aggccggtgc ctggctcgct tcgttgatgc
      721 ggatgggcat cagcagcatc agcacctcct cgccctcacg cctttcgacc ggtttgagga
      781 tcgctgcgct ggtggcatcc ttgagttcaa aaacgacctc ttccccatcg agatgacgga
      841 gcacatccat cagatagttg ctgttatagc cgatctgcag ccattcacca ttgaaagagg
      901 cgggaatgac ctctttggcc tccgcaccaa attctatatc ctcggagcgg accgtcactt
      961 cttcggccgc gaccatgaga cgtacctgat gagtcaggga actggaaaag atggagacgc
     1021 gacgcaggga ggagatcagc aactcgcggt tgatctgcag cctcaattcg ttatccttgg
     1081 gaaggacgcg ctcataattg gggtaggttc cgctgatgag ttttgaatag accaccgccc
     1141 ggtcgagggt gaaaacaatg tgatcctcgc caatctggag gcctacattg gcatcgtcct
     1201 cgaaattgcg cagcagaaga tggagggttt tggtcgggat gatcaggttg cggcgcacat
     1261 cgtcggcgat catgccgttg ctgacggtga ttttggcgag gcgatgaccg tcggtggcga
     1321 caaaacggat ctcgccgggg agcagttcca tattgatgcc cgtcagtgcg gggcgcagtt
     1381 catcggccga gacagcgaac atggtttgtt ccgccatgcg tccaagcagc ccggaatcca
     1441 tctgaaagga gagttcaccc tcctcaacgc tgatctgggg aaaatcctcc ttgggctgag
     1501 tggagagttt gtactcgccc ttctccgtct tgatggtcac cttgttgtcc gcatctgaaa
     1561 agatattcag gggaacgtcc ggcagctcgc gcacaatttc gacaagaatc ttggccggga
     1621 tggctaccga accctcctca gaactgttca catccagggt ggtagtgatc gaaatttcca
     1681 gatcggttcc ggtcagggag agcttgccgc tgcgcagatc gagcaggatg caggtcagaa
     1741 ttgatatagt ggttttggga ggcacaacac caatcacttt ttgtaatgca tcaaaaaact
     1801 ctttttggga aacggtgaaa ttcatgggtt cctcctcaca aaaaagttgg cgcgttttat
     1861 cactaaagtt atataataaa aaaatatata tcaagataat tgtagtagta ggcagcgtgg
     1921 ataagtggat aagggccctt tgacagcgag cgagaatctc ctcttgtgct tgaaaatatg
     1981 aattaacgtg tttctgcaaa acagccgggt tatgcacacc ggcgtgtgga cagaaggctt
     2041 ataaatggcc atttgtccac tattcagcaa gaggaaaaga atggctgtgg aaagagaggg
     2101 gtttgtccac aattttcgaa ggcttgtcca cagcagttta acaacttgtc cacacttctc
     2161 aaagctggga aatttcgatt tggtttttga tcgcctcaac gttttccctg tacttgccat
     2221 ccgtttccat cagctcctct gtgcagttga tggcgtggat gacggtagag tggtcccgtc
     2281 caccaaaatg gaggccgatg gtcttgagcg agctgtcggt gagagatttg cacagataca
     2341 tgcagatctg ccgggcctgg gcaatctcct ttttgcgggt tttggagcgc aggagatcgt
     2401 cgggaaagga aaagtgctga gccgtcactt tttgaatgta ttcgatactg atggccttgg
     2461 ttttactgat gcagacatcg cgcagcactt ttttcgcaac ttccagggtg atatcaatgc
     2521 cgttgagcga ggcaaaagcc agcagacgaa tcatcgcccc ctccagttcg cggatattgg
     2581 aggtgatgtg ggtggcgata aaatggaaaa tttcaggggg aagatcgatg gaactctctt
     2641 ctgccttttt ctgcaggatg gcctgccggg tctcaagatc gggggcctga atgtccacca
     2701 ccagtcccca ttgaaaacgg gatttcaagc gctcctccat gccctgaagt tccttgggcg
     2761 gacggtcgga ggagaggatg atctgcttgc ccttctggtg cagggcatta aaggtgtgaa
     2821 aaaactcgtc ctgtgtacgt tccttgttgc caaagaactg gatatcatcg atcatgagaa
     2881 tatcaacatt gcggtagagg ccgctgaatt ccgtggtctt gtttttctgg atcgaattga
     2941 tgaaatcggc ggtgaagcgc tcactgtcga catagagaac gcggatggat tggttatgct
     3001 ccttggcgta attaccaatc gcctgaataa ggtgggtttt gcccaaaccc acaccgccat
     3061 aaacgacgag aggattgaag gtggttgtgc cgggtgattt ggccaccgcc agggcagccg
     3121 ccttggcaaa cttgttgcat tcgccctcga cgtagctgtc gaagcagtag cggacattca
     3181 gattgtccgc atgcgtgctg gcgacaggga ggggggattt cgtcggcgtc ggcggaatca
     3241 gatccgcggg caggggcgcc tccatgcgga tggaataggc cagcttgaag ccgttgccca
     3301 tcacctggga gagggcgcga ctcatcatcg ccccatactg ctgctcgatc cattcataag
     3361 agaactggtt gggaacctga atggttaagg ccttgtcgtc cagcttgatg ggctgaatcg
     3421 gcttgaacca ggtgagcaag gtattgggat ggacctcgtc tttaagaatg gccaacacct
     3481 tgtcccatac ttcatgatga ttctgataca ttactgctcc tgtagaaaga ggggtaaatg
     3541 tcaagatatt gcccgaacct ctccgtcaca tcccagcaaa ggatataaat caacttatcc
     3601 acattatgaa cagggttatc cacatgaaca aacggaaaat aatagatgat atcaataata
     3661 attataatgt cttgtgatta atgtggatga cagattgtga acaagcggcg ggaaagtggc
     3721 atcagggctg ccaatcggat tgatgcatgt aaaagttatc cacattctgt tatccacatc
     3781 actccgccac taaatgtccg taaaagcgcg ctgatttgca atagcaaatt ttcctttttt
     3841 ctggagggga gagccacagc aaaagcctcc cgcctcgaag aggtagacgg acggagagaa
     3901 aggctggaat gatcgtcgga gcgcttaagg gaaaattaat gcttgatttt ttgtaaaaat
     3961 attcgtaatt atatgagcta atatctattt atcaaaggag aatcccagta tgcaaccgac
     4021 cttccagccc agcaaaagaa aaagggtgaa caaacacggt tttcgcgcca gaatggccac
     4081 taaaaacggc cgcacagtgc tcgccaaaag aagggccaag ggccgcaaaa aactctccgt
     4141 cagcgacgag taattcgact ttgcattgaa aaatttgcga ttggataaat cctcgctcat
     4201 caaaaagagc gcggagttct ccagcatcat ccgccggggc tccgtctata aaggaagtta
     4261 ctttacccta ttcgtcgtca aagaggacaa gctcagaatc ggcttttctg taaaacgagg
     4321 gatgaaatct gtcgatcgaa acagattgaa acgtcgtacg cgggaattgt ggagggcctg
     4381 gagaggggag tttgatttgt cgggaaattt gatcgtcatc actcgggaac aggccctgtc
     4441 cgacccattc aaccttctgg aacaggattt tcgccgtctt cttgaacaga tctccctcca
     4501 cgaaaatcag accagttcct gaaaggtccc gcgcgccttg tccagttgcc ggcacggaaa
     4561 cagtgcgcta tagtgaaaat caatcctgca aaagagcttg tgctgctgct gatccgtttc
     4621 tacaagaagg cgatttcccc gctattgccg ccctcctgcc gctaccaccc aacctgttca
     4681 acctatgccc tccaggccat cgaaaaatac ggactcttga aaggcggagg caaggccatg
     4741 ataagagtgc tcaaatgcaa tccgttcttt ccgggcggaa atgaccctgt ataggaaacc
     4801 attatgaatc tggataagaa gaccattctc gccttcttgc tcatcggcct ggtttttatc
     4861 ttcgtgcaaa gcccgatcta ccagaaactc ttcttccccg aggcttataa aaaacagctc
     4921 gaagctcgcc aggccagacc agcggccatg gactctctcc ggggaaccca ggagcaggct
     4981 gctactgacc atgaagaaat cgccgccaca acggctgatt cctcgcgctc acgggtggag
     5041 gcggaggccc tgatcccgga acggctgatc accatcgaat cggatctcta taccgcacaa
     5101 ttttccactc gcggcggcac cctggtgagt tggaccctca aggagtacaa gaagagcgag
     5161 aatgaaccgg tctccatgct tccgtcagga agcaaaggcg gtctcgccct cgatttctat
     5221 acccgcgatg aggacaccct gcacacagcc ggctaccatt ttaattgcga ggctggtgat
     5281 agcatcctcc tctccgaagg tgatgccacc ctgcgcttca cgcgccagtt cgacagcgat
     5341 cagcgcatca gcaaaacctt cacctttaaa aacggacgct atgattttga tctcgaggtt
     5401 gtcctcgaga atatgggcag cctggtcgcc gacaagtcct accagatcgt catacccaat
     5461 ggactggaat caaccgagaa gcgcctcgga gaggatatgt attatgccaa ggcggccatc
     5521 gcggccgctg gccgcgtcaa caaaatcggc aaaaccaggg gcatcctcga gaaggagagc
     5581 ggcagcatag actgggtggc tgtacgcact aagtacttcg cttacgcggt gatgccgcag
     5641 accaacaaag ccgaatacgc caccatcatc ggacgcgaac tgcccaagag cggctacacc
     5701 aaggaaaaat ggaaaaaatt cgccgtcacg atcggaatgc ccctcacccg cgagaatcat
     5761 cgagatcgct tcaccatttt cattggtccg atgaaggacg aactgctcaa atcctataat
     5821 ctcggcctcg aaaaactcat ggacatgggc gcgaagatca tccagccctt cagtgttgcc
     5881 atcctctgga ccttcaagaa gctccactcc atcattccca actatgggat cgtgctgatc
     5941 atcttcgccc tgctcatcaa aatcatcact gcgccgctta cccagaagag caccaagtcg
     6001 atgaagagga tgcaggccct ggccccgcgt atgaacgaac tcaaggaaaa gtacgccaag
     6061 gatccccagc gcctcaatga ggagaccatg aagatctaca aggaggaggg cattaatccc
     6121 atgggcggat gccttcccat cctcgtgcag atgcccctgc tctgggctct ttacatcgtc
     6181 ttccgcagca ccatctccct gcgccaggag ggttttttct ggtggatcaa ggatctctcc
     6241 ggtcccgata ccatcttcac cctccccttc accattccga tgtacggcaa caccgtcaac
     6301 gtcctcccca tcctgatggg gctgacgatg atcctccagc aaaagatgtc gatcaccgac
     6361 ccgaagcaga aggccatggt ttacatcatg cccatcttca tgaccctgct cttcaacagc
     6421 ttccctagcg gactgaatct ttactatacc ctcttcaata tcatctccat cgtccaccaa
     6481 aagtatttca tgggccttga agagcccgcc ccggtggtcg tgaagagcaa gaaaaaatag
     6541 gcggtttttc ccaatcatgc aaagatgcct cactttatcg ggtggggcat tgttttttag
     6601 ggtggcccga tgagccccta ttctgaaaat gacaccattg ctgcgctggc cacagcccac
     6661 ggccccgccg cccttgcgat catccgcgtg agcggtccgg atgccctcca gatctgtgct
     6721 gcgcttttta cggggcgccg gcggatcaac ctcaagcggg cagatcgcta tcacgggatg
     6781 ctggtggaac cggaggtcgg ctgcggtgag gcggctgagg tctatgacga ggtgatcctt
     6841 cacacctatc ccgccccgca ctccttcacc ggtgaggata tggcggagat caactgccac
     6901 ggcgggctcc tggtggtcga gcgactgctc gggctgctgc tgcggcgcgg ggcgagactg
     6961 gccgcccccg gagaattcag caagcgcgcc tttctcaatg gcaaactcga tctgctgcag
     7021 gcggaggctg tcgccgacat catcgcagcg ccgagccggc gcgctctcgc cctttccctg
     7081 gcccagcttc acggcgatat atcattgaaa ataaacaaat taagtgatta tttacgcacc
     7141 agctgtgccc tgctggagct ggaactcgac ttcagcgaag gcgaggtgct ggccgatcgc
     7201 gcacagttgg aggagaagct gaatgaggcg gccggggagc tgcgcaccat tctcgccggc
     7261 tatcactacg ggcagctgct gcgcgagggg gtcggggtgg tcctcgccgg caaggtgaat
     7321 gtcggcaaat cgaccctgat gaaccgcctg ctccggcgcg accgcgccat tgtctcacac
     7381 ctccccggca ccacccggga taccctggaa gaggggttga ggctgggcgg gtacctcttt
     7441 cacctcactg ataccgccgg catccgggcg gccgccgacg aaatcgagcg ggaaggagtg
     7501 gcccgaacat tggccgcgat ctcctcggcc gacctccttc tcctgatttt cgatgccagt
     7561 actcccctgg acgatgagga ccgggagatc atcgagctcg gccgcgggcg ccccgcgggc
     7621 tccaccctct atctcttcaa caagtgcgat ttgctccccg gaccggagaa atttaccccg
     7681 cctcctatcg catcagtcga atgccttctt gttctctccg tccgcaccgg gacgggggtt
     7741 gaggaactgg agcggcgcat gatcgcccgc gtcgaaaggg agaggccaga gggagaaggc
     7801 gtgaccatca cgcggctgcg ccatcacgat gccctgcgcc gtgcggagta tcatcttgag
     7861 caggcacggc agagtctgtc tgaagagcgc tccggggaat tcatcgccct ggatctgcgc
     7921 atggccctgg cggccgcggg cgagctcacc ggagaggtgc gcagcgccga catcctcgag
     7981 gatatctttt ccaccttttg cataggcaag tagaatgttt cacgtggaac atcaggagag
     8041 cgaaggcagc ggcaggggag cgcgatgaaa ggctccggcg atggctatga cattctggta
     8101 gtgggtgggg gtcatgccgg ctgcgaggcc gcgctggcgg ccgcccggat gggggcctcc
     8161 accctgctca tcacccagaa catcttcacc ctggcgcaaa tgtcctgcaa tcccgccatt
     8221 ggcgggttgg ccaagggcca cctcgtcaag gagatcgacg ccctcggcgg cgccatgggc
     8281 gtggtggcgg acgagaccgc cctgcaattc cgcatgctca accgctccaa gggtcccgcc
     8341 gtctggtccc cgcgcaccca gaatgaccgc tatcactacc ccctgcgcat gcgcaggctg
     8401 ctcgaggagc aggagaacct ctccctgcgc cagcagagcg tgaccgccct gcttatcgaa
     8461 aaggacgaga tccgtggggt gatgaccgaa gcgggccatt cggtgcgggc cggtgcggtt
     8521 atcctctgtg ccggcacctt tctcaacggc ctcattcata tcggcctcaa ccactttccg
     8581 ggagggcgca gcggagaacc ggccgccggc ggcatcgccg aacagctcgc acactatggc
     8641 ctccacagcg gcaggctcaa gaccggcacc cccccgcgcc tcgacggccg ctccgtcgat
     8701 ctgcagcgca tggaggagca gcccggggac gccgagatca tccctttcag cttccgccat
     8761 caaaacctca aaatggacca gctttcctgc tacctcaccc gcaccggcgc cagcacccac
     8821 gacatcctcc gctccgggct cgaccgctcc cccctctatc agggggtgat caagagtatc
     8881 ggaccccgct actgcccctc gatcgaggat aaaatccatc gcttcgccga caaggagagc
     8941 catcagctct tccttgaacc cgaggggcgc gagagccatg agtactacct caacggcttc
     9001 gccaccagtc tgcctgagga tatccagatt gccgccgtcc gctccgtgcc cggactggag
     9061 cgcgcccatc tcacccggct gggctatgcc atcgaatacg actatttcca ccccacccag
     9121 ctcaaggcca atctcgagac caagtcagtg cagggcctct tcttcgccgg gcagatcaac
     9181 ggcacctccg gctacgagga agccgcagcc caggggctga tggccggcat caatgccgtc
     9241 ctcgcccggc gcgggcagga gcccttcatc ctcccgcgcc aggagtccta tatcggcgtc
     9301 ctcatcgatg atctcaccac caaggagctg agtgagcctt accgtatgtt cacctccctg
     9361 gcggagaacc gcctccacct ccggcaggac aacgccgatc tgcgcctcat ggaatacggc
     9421 cgccgcctcg gtctgatcga tgacgctgcc ttcaccgcgc tcgaaagact gcgcgaggcc
     9481 gttcaggcgg ccagggtgcg gctcgagaag gtgaatcctg atccggagcg gatcaatccc
     9541 ctgctcatcc gcgcgggcaa agcccccctc ctgcaccgcg aaagtgcggc gcggctgctc
     9601 aaacggccgg agctgcgtct ggccgacctg gccgggtgcg gcgaagatcc ccttcttgtt
     9661 gaggagttgg accccttctg gcgccgggtg ggcgagcagg tggagatcga gatcaagtat
     9721 gccggctttc tccagcgtca gcaggaacac attcgccgca tggccggcct cgaggaaacc
     9781 ctcattccgg aggagatcga ctatgaggcg gtcccctcgc tctcgcggga gagccgcgag
     9841 aaactggcgc gcatccgtcc gcgcaccctc ggacaggcct cgcgcatcct cggggtcggg
     9901 cctgaggacg tggcggtgat catgatccat ctggcgcgct tgcgccgcag ccgctctgtt
     9961 ccacgtggaa cgcatctccg ccaggaggaa aatggacccg gcgaataaaa aggctttgtt
    10021 ggcgctcgca aatgatgtac atttgcggga gcagcagtgg gaggagatcg cccgctacgt
    10081 cgagctggtc gagagctgga accagcgcac caacctggtc tcccgccagg atatcggccg
    10141 catcgtctcc cgtcatgtgc gcgaatccct ctggtttata cggccggagg tgctcgggga
    10201 gttccgactg gtcctcgatc tgggcagtgg agcgggtttt ccgggggtgg tgatgaagat
    10261 cgccgaaccg ggcctgctcc tcaccctgct cgattcgcgc cagatcaagg cccgcttcct
    10321 cgccgaggtc attaaccacc tcggctggga cgatgttcag gccttttgcg agcgcgccga
    10381 gaatctgccg ggccgctttc ccggtctgac cttcgatctg gtcgtctgcc gggcggtcgc
    10441 caaactgagc gacctctggc gctgggccgc tcccctgctc ggcccgggcg ggcgcctggc
    10501 cgccctcaag gggggggatc tggctgaaga gctgcaccag tttggcaaac gccatccggc
    10561 cgtgcgctgg cagctgatcc cgatgccgga actgccggat gatcccgctg cgggccggca
    10621 aatggtcatc gtccacacgc gaatataact gaaacgggag gaacccgtgt ccaaaacggt
    10681 ttttgacgag atcaagaatc tcaccacaga agcgcgcaat cccgctaccc ggcacattga
    10741 ccagcagtcg atctctgagg tcctccatct catcaatgat gaggatctca agatcgcggc
    10801 agtggtacgc agcgaaatca gatacattga aaaggcggtc cgtattgtcg tccaggcctt
    10861 caaggagggc ggccgtctaa tctatgtcgg tgccggcacg agcggccgcc tcggcgtcct
    10921 cgatgcctcc gaatgccccc ccaccttcgg ggtgagcccc gaactggtcc aggggatcat
    10981 cgccggcggc tacaaggccc tgatcagcgc ccaggagggg gccgaagacc gttatgagat
    11041 gggggcgcag gatatcctcg cccgcaacat cacccccagg gatgtggtct gcggcatcgc
    11101 cgccagccgc cgcaccccct acgtgatcgg cgcgatcgac aaggcgcgtg aggtcggctg
    11161 caaaaccctc tacgtcacct gcaatccccg caaggagatg aatatcaagg tcgatgtcgc
    11221 catctgcgcc gtcgtcggcc ccgaggtggt catgggctcc acccgcatga aggcgggcac
    11281 ggcgaccaag ctgatcctga acatgatcac caccaccgcg atgatccagc tcggcaaggt
    11341 gtgggagaac atgatggtgg acctgcagat gacctcgaaa aaactcgaag agcgctccaa
    11401 aaagatcgtc atgcaggcga ccggggtgga ctatgagagc gctgagaagg tgctaaaaaa
    11461 ggcggacgga cacgtcaaga ccgccctggt gatgatcagc gccggggtga cggccgagga
    11521 agcgcacgcc ctgctcaaga aaggggaggg ttttgtccac cgcgccatcg ccctgcatcg
    11581 ttgaggcggc gaccaccgcc gtgccgtctg ctcagccccc gttccggtgg ggggatctct
    11641 ccattctgct gaagccttga ttatgtctgt tatcgacacc atcgccgaca tcttcgcccg
    11701 cagcgaggcc gtggcccgcc tggagaaggc catctccacc gggcagagca tcgtcctgcg
    11761 cggctgccag ggtgcggcgc gcccctttct ggcgcagcat ctccgccagc acgcgcacgc
    11821 ctccttcctc tgtctctttc ccggagcgga tgaggccaag cacttttacg acgacctggt
    11881 cgagctgctc ccgcccgagc tgctcgccct cttcactcca catggcaaac agatgtggaa
    11941 tgaggtcggt cccctgggta cggttgtggg ccagcgcctc aatacgctga atatactcct
    12001 gcgccgcgag cccgccctca ttgtcacctc cgcccccgcc ctgctcgaga aggtggccga
    12061 tcctgtccgc gtcgccgggg ccgcgctcag gctggagcgc ggcatgaact atgccttccc
    12121 acgcctgatc gaagaactgg tccacatcgg ttatacccgc gaaatgaggg tggagaggcc
    12181 gggggagatg gctgttcgcg gcgggcttat cgatcttttc ctcttcgaag agacccaccc
    12241 catgcgcatt gaattctggg gagaccagat tgagtcgatg cgctacttcg acatcgaaag
    12301 ccaacgctcg ctcacccccc tggagtcaat cgtcctgctt cccctcagtg cgggcggctt
    12361 gtacgggccc gcccaggatc cacccttcga tcagctgccg ctcacgtcgc tgctcaccga
    12421 ctatctgccc caggacgtca aactgcttct ctttgataat atattaatta gtaacgagtt
    12481 agaaaacttt gaaaaagagt attcggttcg ctttgatacc tttctccagg atcacccgtc
    12541 cgagggcggc cacttttccg ctttctacag cgacaggaag agcattctgg aaattatcaa
    12601 cacaatacaa acaattgaat taaatacatt tagcacacaa tcctccctgc ctgaaatcga
    12661 gttgtccaca ttgcgcaacc accatttcgc cggcaatctc aagccctttt tccaggaggt
    12721 ggaggcgcat catgaggagg cccggcaaca gggcatcact ccgatggttg cggtcctgtg
    12781 tgattcgcaa gcccagaccg gccgtctgcg ggatctcctc gcccgtgagg agatggggga
    12841 tatcgccgtc gaaaccctca atctctccga gggttttcag tggcccggag agcatctctt
    12901 tctctacacg gaccgcgaac tttacggccg catccgtctg cgcaaacagg acaaggcctc
    12961 gcggcgcatg gtctcctttg ctgatctgct caagctccag gagggcgact atgtcgtcca
    13021 tatcgagtac ggggtcggca tctttcaggg cctcaaacag atcgaggcct acggccgcac
    13081 ccgcgaatgt attcagatcg aatacgccaa cggcgacaag ctctacgttc ccctcgaaaa
    13141 aatggatcag gtccagaagt attcctccag ggacggctat gtgcccgtgc tcaacaagct
    13201 gggcggcaag gaatgggaca agctcaagtc caggaccaaa gccagggtca agcaggtcgc
    13261 cgagcagttg atcaagctct atgccgtccg caagctcagg gccggccacc ccttctcccc
    13321 ggattccatc tggcagaaag aactcgaggc ctcctttgtc tatgatgaaa ccctcgatca
    13381 gctcaatgcc gtcaacgaga tcaaggtgga catggaaaaa cccgtgccga tggaccggct
    13441 ggtgtgcgga gatgtcggtt tcggcaagac cgaggtggct gtgcgcgcgg ccttcaaggc
    13501 cctgaccgac ggcaagcagg tggcggtcct cgtgcccacg accgtgctgg cccagcagca
    13561 ctttgccacc ttcagtgaac ggctggccaa gtttccggtg cgcatcggcg tcctctcccg
    13621 ctttaaaaac ccgcgtcagc tcgctgaact catccagcag gtgggacgcg gggagatcga
    13681 tctcatcatc ggcacccatc gcctgctttc ccaggatatc cgcttcaagg acctcgggct
    13741 gctgatcatc gatgaggagc agaaattcgg cgtccttcac aaagagcgcc ttaagatgct
    13801 caaggccaac gttgataccc tcaccctctc ggcgacgccg atcccgcgca ccctgcatat
    13861 ggccctgatg ggggcgcgtg acatgtccct gatcaatacc ccaccgggca accgcctgcc
    13921 gattgtcact gaagtgtgcc ggttcgagcg cgatcacgtg cgcgaggtga tcctgcgcga
    13981 agtcgagcgc aaggggcaga tctttttcat ccacaaccgg gtcgccacca tctcgggcat
    14041 ttatcagctg ctcagcgagc tcgtaccgga ggtcaccttt gcggtggcgc acggccagat
    14101 ggaggggccg gagctggaga aggtcatgac ctcgtttgcc gagggtcgca tccagtgcct
    14161 ggtctgcacc atgatcattg aatccggcat cgatgtgccc aatgccaata ccctcattgt
    14221 caaccgcgcc gaccgtttcg gtctggccca gctttatcag ttgcgcggcc gcgtcggccg
    14281 ttcagaccat caggcctacg cctactttct tataccgccc ctgcgcaaac tgacccgcac
    14341 ggcgatcaaa cgcctgcaga ccatccaaga gtgctcccat ctcggctccg gttacaagat
    14401 cgccatgcgg gatctggaga tacgcggcgc cggcaatatt ttcggtgcgg aacagagcgg
    14461 ttttgtcgac gctcttggct acgagctcta tgccaagatc atcgaggaag ccatcaagga
    14521 gctgcgtgag gagctgaatc ttgcgccgca gcccaaggtg gaagatgatg tgcaagtagc
    14581 gatagaatca agggttgaaa tgacgggcga agcctttctg ccccccgatt acgtcgaagc
    14641 cgcctctgat cgcgtcgata tttacaagcg cctgatcgag gccaaagcca tgcccgccat
    14701 cgacgatctg gagcgcgaag tcctcgaccg tttcggcccc ctgcccgagg cggcccgcaa
    14761 cctgctcgat tatgtccgca tcaagatgct cgcccatctt tccaaagtgg aggagatcgg
    14821 ctgcaaggat ggcaaaatca acggcaaatt cttcgccgcg gctgtgccca agggggaaca
    14881 attccggccg tggataggta aaattgtgca aaaagcctgt tatccttttg aacttcgcca
    14941 gatccagaac agccttcact tcgagttcaa gacggaggcg ggcctagcgc ccctggccca
    15001 gattaaaaag ttcttggaaa gtattatata attactatat ttatctaaat ttagccttta
    15061 caaatcggcg gaagccagtc ctatgagaac agggagtgcc ctggcggcga gccttcttct
    15121 tttttccctc acctgcctga tgtgtacaaa gggagaagag gcgggcatcg tcgctcgcgt
    15181 cggtcagtcg gtattgaccc tgcaggaggt gcgtgagcgc aaccccggcg ccgggaccgg
    15241 ggccgccaga agccaggccg aacgctatat ccagcactgg atcgaatcgg aactgctcta
    15301 ccaggaggct ctgctgcagg gcgtggacaa gcaaccggaa gtccagcgcg ccctcaagga
    15361 gttgagccgc gattatatca tctccgcttt ctccgagcag gcggcgaacg ctgcagtctc
    15421 ggtcagcgat gaggagattc agcgctacta tcaggagcag aaggaggagt tcctggctga
    15481 ggccgacctc taccaccttc tgctcattct ggtacaaaac cagaatgagg ccctgcagct
    15541 gcgccgtgaa ttgatcgggg gtacaccctt cagcgagatc gccagccgta attcgctcga
    15601 cggcagccgc ttgcacgggg gcgatctggg ctatgtcccc ctcagcgccc tctcgcccat
    15661 gctcgcccgt gcggttagtg cgatgcagcc ggggagcctt tcggcgcccg tgaaatcgga
    15721 gttgggttac aacctcctcc ggctcgtcga tgtgcagcgc aaaggcgcca cccgcgcact
    15781 cgaggaggtg cagccggtca tcaagcagcg catcaccgcg cgcaagaagg acgaggcgct
    15841 gcagaacctg atttcgcgcc tgagcagcga ggccgccctc tacaccgaca tgaccaaact
    15901 tgaaagcctt gaaggaaaac aatgacgatg aattttgcca aaagggtgtc acgcatcgcg
    15961 cgtggtgccg cggcgggcgt gctgttcctg ctcgcactgc ttttgaaccc cctgcaggcc
    16021 cagcaggttc tcgaccggat tctcgcagtt gtcgatgatg atgtgattct ggaatccgaa
    16081 gtggcgcagg gcgcctacct tacggccatg cagtaccgga tcgatccggt caaatccccc
    16141 cgtgaattcg cccgactcaa gcaggagact ttgaccaatc tgatcaacca gaagatcctg
    16201 ctcgtgcagg cggacaagga caccgtcaaa gccgatgagc agcaggtcga gcactatctt
    16261 cagcagcaga tgcagagcgt gatccagcag ctggggggcg aacagcaggt ggaagagtat
    16321 ttcggcgcct cgcttgccaa ggtgcggcgt aactaccggg aggagatcga gcgcaacctg
    16381 cgcatccgtg cagttcagtc cgccaagatg caggggatca agatcagccg ccgggaggtg
    16441 gaggagttct tcaaggtgta caaggactcc attggtccgg tcaaggagtc tttcgatctg
    16501 agccacatcc tcatcaaggc gttgcccggc gaagcggcgc gccaggcggc cagggacaaa
    16561 gcggaggctc tgcgtcagcg ccttctggcg ggcgaggatt ttgccacgct tgcgcgggag
    16621 aattccgagg atccgggctc ggccgcccgg ggcggggatc tcggctttat gtcgcgtggt
    16681 gaatttgtcc gcgattttga ggaggcggcc ttcaagctgg aagcaggcgc catcagcgag
    16741 gtggtcggat cacagttcgg ttttcacatc attcaaatgg tcgagcggcg gggtgaaaaa
    16801 ataaacaccc gtcacattct catcacctcc aagccctccc gggaggatga ggtcgccgcc
    16861 gccgacacca tcaaggcgat cgcggcccag ctgaagcagg gggccgattt tgagcagctg
    16921 gttgctcgtt attcacagga tgagaattcc aaagaggaca agggtcatct cggctttttc
    16981 gagatcgacc agctgcgcga gcgcgccaag gaactggtct acgccacgac ggggctgaag
    17041 gcgggtgaat actccgatcc ggtgcggacc cagtacggtt ttcacatcct ccgcgtcaac
    17101 cgccgcgagg aggcacgcga gctggatttt aaaaaggatt atgaccggat ccagcgcctc
    17161 gcgctcgact acaagacggg gctggccttc aacgcctgga tcgaggaact caagaaggat
    17221 gtgcacgtcg aagtcaagga cgatctctcg aactgatttg tgatcaggcg gaattctctt
    17281 ttcaccaatc ggctcgcttt gggccgattt tttttcccgt cacagggagg ggatgcggat
    17341 gactaaacgc ggccgatggt ggctgcttct cctgggcgca gccctgattc tgccaatcga
    17401 gcggctctct gcggacgctt cggaattcac catcgcccgc ctcaagtatg gcgggggcgg
    17461 ggactggtat aacgatccct ccagcgtacc caatctgctc gccttccttc gcacccgctc
    17521 ctctctggcc acggcgaagg aggaggtcca tgtctccctg atggatgacc aactattcgc
    17581 ctgtccattc ctctttatga ccggccacgg caggatcgcc ttcaccccgc aggaggcggc
    17641 acggctgcgg atctatctaa ccagcggcgg ctttctcttc gccgatgatg attatggcat
    17701 ggatgagcac tttcgtcgcg aaatcgccaa ggtcctgccg gaccatgagc tgctcgaggt
    17761 gcccttttcc caccccatct tccgatcgcc cttttccttt cccgagggat tacccaagac
    17821 ccatgaacat gacggcggtg tgccccaggg cttcgccatc tttcatgagg gcaggatggt
    17881 tgttttttat gcctataact gcaacatctc cgacggctgg gcggacccgg aggtccacca
    17941 tgatccccct gaggtgcgcg aacaggccct gcagatgggg atgaacatcg tcgtctatgc
    18001 tctgacacat tgaaaggcag gttgctgaga ggcatgcagc aggagggatt gaaaaggctt
    18061 tcggctttca gggaggcgga gaagaggctg cgggtcggcg ccgctttgtc gcgatgggct
    18121 acacatgcgc ttcttttact gctggttctg gtattactgc agtccctgct ctccctgccg
    18181 ccagcgggcc gttatcttct cctggccttc gctctgctgc tcctgctcgc ctcctggctc
    18241 aaacatgtgc tgcggccgct gaccgacctc ctcttccgcc cggagcaccc ttccctcaac
    18301 agcatcgccc tgcgcatcgg ccgggagtat ccgcagctgc aggaccggct cgccaatgcc
    18361 ttgcagattt ctgccaggcc tgaagcgaac gatgtcaagg tctccccgga gttgatcgag
    18421 gccgtgcgcg caggcatccc cgcttttctc gctgacatcg atctctcgcg tcatctcgac
    18481 cgcgctccag cctgccgttg gctgcgcagg ctggctgctg cggccgccct ctttctattc
    18541 gcctggctgc tctttcccaa aaccctcctg atcggtctgg agcgcaccat ccatccccgc
    18601 cgggggctct ccggccagct cgcccctgaa atccgcgtgg aaccgggcga ccgcatggtt
    18661 gtccgcggcg caccggtcac tatccgcgcg tggacggaga ccctgatggt tggggatctg
    18721 caaatcgcca tcgaacggtt cgggcggcga gaactcctct ccatgacccg cggcatgagt
    18781 gatacctttc gctacgccat tcccgcactg agagactctc tgctctacca gatcctctat
    18841 caaaagtcgg ccaccagaaa gtatcttctg cgggtcgtgg atctgccgat gatccgctcc
    18901 ttgcagctga gtctgcagcc gccgtcctac agcggcgccg agcctttcct gctggaggag
    18961 aatatcgggg atgtcagtgc cctgaaaggg acgcgcatcg agtggcgggc cgaggccaac
    19021 aagccggtca cagaagccag tcttcgcttc agctccggtc ggatcctgcc cctggaggtc
    19081 aagggccgtc agcttgctac tacctttgcc ctgcagggcg aggagagtta ttacgccgaa
    19141 ttgaaggaca actccggact ggcgagcgag aatccgatcg tctaccatct ccgcctgctg
    19201 gctgaccagt atcccttcgt ccgcctcctg gccccctcgg gcgatgtcga tttgcacgag
    19261 gatatgcgcc tctccctgct gatccaggct caggacgatt acggcctctc ggggctagcc
    19321 atcggctacc aggtggtcga ggagaaccag gcaaaaatcg attcgagcag attcagacgt
    19381 ctcgagctgc ctctccatcg ttccgggcag gccatcatca acctggccta cgactgggat
    19441 ctctcgtcct cgcctctctt gccgaccgaa gccctgctct actacatcga ggttcgcgac
    19501 aacgatacgg tcagcggccc caagtcggcg cggacgggcc tctaccgggc gcgctttccc
    19561 tcgctctatg agctttacaa ggaggtcaac agtgagcagg atgatgcgat cgccggtctg
    19621 gaggagaact atgagaaggg gctggagctg aagaaaaagc tcgatgagat ggcccagcag
    19681 ctcaaacgcg ccgacgaact ctcctggcag aagaagcagg aggtcggcga tcttctcaag
    19741 cagaaagagg agctcaaaca ggagctggaa aagatagctg gcaatctgga cgagatgatc
    19801 gagaagatgg aggaaaacca gctgctttca ccggagaccc ttgataagta ccaggaactc
    19861 caggagcttt accgtgagat catgaccccc gaactgcaga agaacctgga gaaggttgct
    19921 gaagcgatgc agaaggtcga tcccgcccag ctgcaaaagg ctctggagga gttcaagctc
    19981 accgaggagg cgctcaacaa gagcctggac cgcaccctgt cgctgctgaa gcggctgaga
    20041 atggagcagc agctcgatca ggccatccac atgaatgagg atctgcagaa gcgccagcgc
    20101 gagctgaccg aaaagacgat gcagagccag ggtgaagagc gcgaaaaact cgccaaagag
    20161 caggaggggc tgcgggagga tctgcacagg atggaagagg ccatggctga ccttcagcag
    20221 gagatgggcc gcgagccggg catgcccgag gagagtgtgg ctgcggccag ggaggagctg
    20281 gccaaagctg gcctgcaagg gcagatggag gccatgcagg gggcgatcgc acaggggaac
    20341 agcgaccgca tgcagagcgg ttcgcagcag gttcaggcgg gtctggagag gagccgcgag
    20401 cagctgcagc aggccaagga cgcgatgacc ggagccatga tggagagggc catgcgtgct
    20461 cttcagcgcg gaacccggag cctgcttagt ttatcccaga tgcaggagga gctgctgcgc
    20521 agttccgccg ggatgcccca gggcagcgcc cgcgtgccgg agacagccga gcgtcagcag
    20581 cagctctcct ccagcctcga gagggtcatc aacgatcttt atgccgcttc aaaagagagt
    20641 tttggtatca cgccgcgcat cggcaaggcc ctcggccagg cgcagcaggc catgcagcag
    20701 gcgctgcagg ggctggagaa ccgcgatctc gggcaggcgg gccagcggca gggtcaagcc
    20761 atggccggac tcaatgaggc cgtgctgcag atggacgcgg ccatgcaatc gatggcccag
    20821 ggccagggcg ggggtatgag catgggccag ttcatgcagc agatgcagca gctggccgat
    20881 ggccagcagg ggatcaatgc ccaaaccctc agcctgagcg gaggaggaga atccctctct
    20941 ctcgcccagc aggcggcgat gtcccggctt gcccagcagc aaggaggtat acgcaagtcc
    21001 atgcagcagc tcgcctccga ggcggcgggt ctctcggacc tgcttggcga tctggagcac
    21061 atcggcagag atatggagaa ggtagagaag gatctggccg ggcagcgggt cgatcgcagt
    21121 accattgaac gccagaaccg cattctctcg cgcatgctcg attaccagcg ctcgatgcgt
    21181 gaacgcgaac gcagccagga gcgcaaggcg gagaccggca aggagtatca tccctccagc
    21241 ccgggggagc tgcccgggga tctcggccag cggcgggacc ggctgcagca ggatttgctc
    21301 cgtgccaagg aggagggcta ttcccgcgat tatctcgaac tgattagaaa atattttgaa
    21361 tccctcggag aacgtgaaga gaagcgttag atatctgttg atcatcgccg tgctcctggc
    21421 cgccctctcc cggacggctg cacaggagaa gcggccggga gatcaggagc tgcgcatcct
    21481 gcaacagcgg gcgcagcagc tggaggctgc aggccgtctc gaccaggcgg gcgaactcta
    21541 cagccgggtg gcgagaatcc tgccgccgcc gcaaaatctc aacgcctatg ccggcgcgcg
    21601 ccgctgtttc gagcgcagca aggatctggc gggctgggag gcgctcatcc atgatctgca
    21661 gaccagacag cgcgccctgc ccttccaggt tgatctcgcc gagatcgcct atctgcgcgg
    21721 cgaccgcgcg gaggccctgc ggacgtggag agcagtcatc gacgacaatc cccaggagga
    21781 gcaggcctac aaactaaccg gtgcggttct gctcaagtac tacctgtatg acgaggccga
    21841 gagcctctat ctgcgcggac gcaaggcttt caaggatccg cagcgctttt ttttcgagct
    21901 ggtgcatctc tatcaagatc agggtgcctt cgcgcgcatg accgaggagt accttaacta
    21961 tctgcaaaaa aatgcgggcc agcttggcta tgtacaggcc caactgctct ccgccgggga
    22021 tgaacccgag gctgtccgcc agatcgccgg cagcatcgaa cgcgagatga aatcctccgc
    22081 ggattttcgt cttccgggct gccagctgct gggttcgctc tacacccagt cccgcgatta
    22141 tcgcctggcg ctgcagtgtt atgaggagct cgaggaggcc gcacgccgca agaatccggc
    22201 cgaggtcggg cagtattact acaccttcgc catggctgca atgagcgatg gcgcactgtc
    22261 cgaggcgaga agggccctgg aggccctggt gcagcaggcg ggacccagga gcccacaccg
    22321 tgttcacgcc gccttcgcgc ttgcccggct gct
//
LOCUS       DAOPWU010000019        22176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_2345_length_22176_cov_4.745219, whole genome shotgun sequence.
ACCESSION   DAOPWU010000019 DAOPWU010000000
VERSION     DAOPWU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2345_length_22176_cov_4.745219"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKM23_02230"
     CDS             <1..238
                     /locus_tag="PKM23_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90321.1"
                     /translation="LCLPLTDTALAIIRRVRRGIHPFHADKEHIHHRLVRLGLSQPGA
                     ALFMVGLSLIFGILAFLLAQGMHTDFRFLGQLLP"
     gene            349..5997
                     /locus_tag="PKM23_02235"
     CDS             349..5997
                     /locus_tag="PKM23_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNY90322.1"
                     /translation="MLPAAIFLAVFFISAHASAQSRAGLTLVSSSPLERIYEWQAPEW
                     EWQSVIIDGEACLRPGASIATLWSRPGDPLLPMEALHFDLPPGQSAIITLTDSLFSTE
                     AAQLPLCPAPVIDPESGNLVYSINAMSDRSGLVPESSWNSTIGEARGRRILAFSLHPV
                     QYDMQRRAFRLLQYARLKIRFTIPESSLLRPVIQPPQTAAFSPLTKIALVDEGVYEVT
                     GAALQAAGVALGSLSPQNCQLYHRGVEQVIEVEDGGDGRFDLEDRLLFYGRPRRGDVE
                     FYDAYSDTNVYFLASGSMPGLRFAPVAAGSSIAAAADIFPDTLHLERDLAYYAGDSDN
                     DIHNSERVPGEGWVWSVLNKGGSAALAFTLTAPAAGQDSARLRMRLRGTTRDANGPDH
                     HVVLSLNNTQIHEAWFNDREELIVQTGFRADLLRDGGNTLTVKLLADGPSERSQVYVD
                     WFEIACLRTLAAREDWLNFTPDAAAEKQVFVAGFADSSIRAWDLGRLRAYLPDHAGRQ
                     WRDRLSVTSGGYLDGNSARLFRSGKEAGYGYRGHNLWRIDPRDGRLVEGRNFDTYSAK
                     AQADSMAAWIQRLPAGTLVAAAIRDEGSVSMNEAAHLALESLGSALTRSVGTRDSWAL
                     LGRKGAAPGSALEVLKKSQSGPAVIDTVLVFPEGASSFSTGLHLPADAGGEVVIFSEK
                     GLKSPPRLSRCTEGDLISGGAGADYIIITHPLFEAEARQLASYRAARNGWRTRVVLVE
                     EIYDAFNHGLADPSAIRAFLRHVRENWPEPAPAYLLLFGDASWDPRNNMKVAHPTEFV
                     PTYGNPVSDSWFGCLDGEGDILPDLHIGRLPVQTPEQARIILEKITAYEAAPLARWKK
                     EFLFISGGFDFLEQNQFGQQSAQLIKQYVTAAPTFGSATLLNKTSDGLEEGEHRQEII
                     DTIDSGVAWVNFIGHAGSRTWDLMFHNVDIEALNNAPRFPFITSMTCHTGRFAEPNQV
                     AFGEHFLLAGDRGAIGFMGTSGWGYSYEDYTFLRKLFPAALKDTLRSLSEIIDAAKVQ
                     LWAEAGSSLQIRDMLYQYNLLGDPGIKLAVPVVPDLAVQPADIEVIPEMPSEADSTAR
                     IKVRVHNFGLGTRDSVEVRLLADHPNLGEQVIAPAIKLPPLGRVDSLECLWPLRNLAG
                     AVDVIARLDPADRFLEADEENNQASRSVTVLSSKIQLLLPSPFAVLPAADAMVRLDGS
                     NLGLKGEAIYRIQIDTSLTFASSLLMADSIVASSAPAFDWRPPNLQRDQLYFLRISNS
                     SNEPGYWQVSSFKTSVLAENGWRQQGNYAFAQNGRDNVESAGTAALALLPLPLYAESA
                     GYADGNFTRILVGDAPAIQPRRGHNLVVVDPGTFKVEATRNFDTYADSSAANQMAALI
                     EGLVAGKIVLAGIMDEGTVNMTERAFRALESIGSAQCRSVGVRSSWAIIGRKGAAIGS
                     VPEKTVPPNAGSAAVVDSLYYYPPRGTLTSPRIGPATAWRSVRAEAEVPQQCSLTLSL
                     LGQRRSSALWDTLRTGLPVNFDNDISAISAREYPWLQLVADFSTADQLSTPRLHAWQV
                     LHDPASDLAVSPAFLSLSADSVLAGQPVVLKLDIFNIGLARADSVRITFSESAPGSDE
                     KLFSRVTLPGSLAADQKLTIDQLYTPAGKPGSRLLTIRVDGDNRINELSESNNTVTTR
                     VQVVADTLQPQIEITFDGRTITPGDWVAAQPLIRARLIDDSPLSVADTLQINLLIDGL
                     RVPFSGENGVQLLAPPDTLAIALLQHRPLLRAGEHSLEILFTDASGNINNTRIDFSVA
                     AGLELQRVMNYPNPLSDHTDFTFLLTRPAEVRIRIYTVNGRLIRLLEAGSLGAGFNRL
                     FWDGRDGDGDLIANGVYLYRVEARSGSEHAEVVEKCIIMR"
     gene            6153..7238
                     /locus_tag="PKM23_02240"
     CDS             6153..7238
                     /locus_tag="PKM23_02240"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNY90323.1"
                     /translation="MRYLTYLTALTAALAASLVLVRAAMALAVTWDIQDHPIGRKDHA
                     RVTPLLGGLGIYAAFLLALAAGLGLLWWCGRSPQAAAQFPMLARQAGLLGGVLPKLAM
                     IMAGATLLVVVGLIDDIRGITFPPAIKFVAQLAAAVIAVAGGARSSFLPLFWLDILFS
                     ILWIAAISNVFNFLDNMDGLSAGIALICGLLFFWLTASQGQYFSALLFALLCGALLGF
                     LRYNIHPARVFMGDAGSLFIGYLFGALSLTSSYVVAGSASLIPAIIPVIVLGVPIYDA
                     VTVILIRIREHRPVYVGDQCHISHRLLRLGLSRNQAVVFVLLICIAIGMSALLLPYLS
                     LRLSIMVLLQTILIYVILTLLIRAGRK"
     gene            7254..8228
                     /locus_tag="PKM23_02245"
     CDS             7254..8228
                     /locus_tag="PKM23_02245"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNY90324.1"
                     /translation="MKALITGGAGFIGSHLAERLLERGDEVCVIDDLSTGKLENVAHL
                     RDNPRFHLAVETILNETVMDRLVSECDVIYHLAAAVGVELIVKSPVETIERNVLGSHV
                     VLRLASRYLRKVLITSTSEIYGKSDSIPFSEEDDRVLGATTKSRWSYSCSKAIDEFLA
                     LAYHKEKGVPTVIMRLFNTVGPRQTGRYGMVIPRLVTQALAGRPLTVYGDGQQVRCFT
                     FVEDVVWAAIRLMEEPAAVGQVFNVGNDKGISIADLARKIVEMTGSKSEISFVPYSEA
                     YEEGFEDMRIRVPNLNKVRETIGYEPRVQLDEILRRVIEHFLAKKGQD"
     gene            8793..10277
                     /locus_tag="PKM23_02250"
                     /pseudo
     CDS             8793..10277
                     /locus_tag="PKM23_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
     gene            10390..11676
                     /gene="eno"
                     /locus_tag="PKM23_02255"
     CDS             10390..11676
                     /gene="eno"
                     /locus_tag="PKM23_02255"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNY90325.1"
                     /translation="MTTIIDVFAREILDSRGNPTIEVDVALEGGAMGRAAVPSGASTG
                     EHEAVELRDGDAARYKGKGVLQAVKNVNDIIADEVIGEDATEQTLIDRILIELDGTPN
                     KAKLGANATLAVSLATAKAAAEAFGLPLYQYIGGSNAKVLPVPMMNILNGGMHADNNV
                     DLQEFMIMPAGAPNFSEALRMGVETFHSLKGVLKKKGYNTAVGDEGGFAPDLKSNEEA
                     LQVIMEAIEKAGYKAGKEIFIALDPAASSFYLTDKKVYNLASEKKKLSSEEMVDYYAN
                     LVEKYPITSIEDGMAEDDWDGWKLMTKKLGKKIQLVGDDLFVTNTKRLKKGIDLGVCN
                     SILIKLNQIGTLTETLDAIEMAKTASYTAVVSHRSGETEDSTIADVVVATNAGQIKTG
                     SACRTDRICKYNQLLRIEENLGEVGHYAGMGVIKRK"
     gene            11739..12059
                     /locus_tag="PKM23_02260"
     CDS             11739..12059
                     /locus_tag="PKM23_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNY90326.1"
                     /translation="MNAKRGTFAGFKRRLGKIKVTRGRFYLIGFVILFFGYVAGSSGF
                     VAQIRLWQEGRQLQKAIELEQKKKKWMQKEAESLTNDRNRIKREAQKEHSLGEPDEII
                     VHVQ"
     gene            12121..13983
                     /locus_tag="PKM23_02265"
     CDS             12121..13983
                     /locus_tag="PKM23_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90327.1"
                     /translation="MAFFVVFCCFLFYGRAAAQGMAAAGEGPGLNMPLSLRYRQELNN
                     HLWQFDAGRVVVLGRHWEWSFQEQFRTSMLRLGGSEDKWKDDHSFSTALGFLLLPSLR
                     VQTLFRSLSFFDRQTGLNNDVRSNAVGLGALYTPTPRLRLAGRAGPKWESRMGFRDRG
                     FFLAGEGAVSGLDWGGYLNHLQGLVEMDRFSQRKNHEANVAYAVARVFSQASSDSLRL
                     YTGSRRRDNYTSWLGDVESLREGNKGFENILTYGLGDWSRLLLRTSWQFRNVELLSFG
                     MEELARQRKRNDRLGDHALELAAARNRMQGRMALSYNTQRQKYDIAASGAQSPFSKYT
                     AFVTPDNSSSRLQLSGELSTPVGRRDSLYAYASVSRFQYDTPDTNNFDDRDELRINSQ
                     FIFGRQIRPELRLEVQASVSLYHMVYIFGERSADNSWNRILRLRPVLRYAPSQRLQWH
                     NAFEVLANYVDYDFDNLALQTRSFVFRKFAWDDSLRLEVTRRSSLRLDYRLQLEENGQ
                     LYWEAWKERLLTTRTSHWLQLRVLYTAAPSLSLGPGYGFYSRLEWRHDIDALGRERVA
                     CAQSFNSHGPALSIYYTPSPRTRFVLDAHRRRVKPWQQEPYTINTLDVRLDWLF"
     gene            14049..14489
                     /locus_tag="PKM23_02270"
     CDS             14049..14489
                     /locus_tag="PKM23_02270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY90328.1"
                     /translation="MSEAKLEMHEMIIPSLPEQIQRVEEKCEQMAGLAGFADDERDAI
                     AIAITEMVANAIYHGNKSNPKKSVRIRFYLQTGGLTVHITDQGEGFDPKAVPNPLLPE
                     NLLKDNGRGIFIVRSMMDSVEYRFEPGGGTTVILTKCKKSDVRD"
     gene            14476..15831
                     /locus_tag="PKM23_02275"
     CDS             14476..15831
                     /locus_tag="PKM23_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNY90329.1"
                     /translation="MCGISREPLQAGVRRHPCAALALSLFLVALCFTPAPAATPPQFP
                     DSTLIESILFVGNDRTREEILTREMQLREGDPFDPRIAEEDRQRIENLGLFTRVELEL
                     LPGAGGVILLYILSERWYLFPYPLLFIHDRDWSKLSYGAALRHDNFRGRAVVVNTNFW
                     FGYNPSASLSYTNPWIHSRHRLAFTAQFVYNRVENLSPNLSDFTEKHLGAAVGLSKRL
                     GSDTRVGAGVGYREIHLPPELGLTRSAGGTDRIGQLSASINYDSRDYSPWPGRGWYVD
                     AGVAHKLVLSGSDYLFAIGDVRRYQPLPGGGTLAGRFRIDWSSGRVPLYNLLYLGYSE
                     RVRGHFREVVEGDGRMVAGAELRWPLLKRRFFNLAGADQGDGYANYFRNLPFALHATL
                     FYDAGLIADGGWSRAEQRRLGGFGAGLAVQLPYVELLRFERAWDLAGRGEYIIDMKVW
                     F"
     gene            15834..16562
                     /locus_tag="PKM23_02280"
     CDS             15834..16562
                     /locus_tag="PKM23_02280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNY90330.1"
                     /translation="MAHIHFFFVDPADVGPGQLVLRGAEYNHAARVLRKQAGERIIAV
                     DGCGRRYEGPIVALGSGELTVAIESAVEGGGEPRLRLTLAQGLLKGGMFDWVVEKGTE
                     IGISVFQPMITAHTVVEGRGREGRWQEKARAAMKQCGRSLAPVVRPVAGFARALEAAG
                     GEALFIAWEEAEVAPAGLAEALAGRTAATLFIGPEGGFSEEEVALASAAGARTISLGP
                     RRLRSETAALTAAVRLLDAAGELA"
     gene            complement(16592..17302)
                     /locus_tag="PKM23_02285"
     CDS             complement(16592..17302)
                     /locus_tag="PKM23_02285"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HNY90331.1"
                     /translation="MDYFQLLLLAHVIGDFPLQTDSLFRLKQKSAWGVPLHVLICSLV
                     NVALFFPLLDRPAVWLVILFIALVHFTLDRTKLFLALFHARDGLGYFFIDQGLHLISL
                     LGGAWYLQATLPQDSYWLSSWLTTTLTALVIAGFAAPPIIHYLQLGLAGKTARLKGHA
                     FPAFRHRLPGILSRLLATLGLILGGWYNLLVLTLLSALMLPSPAPGRSPRYRHAELAV
                     SAAISFFCALFAWYVRAR"
     gene            complement(17330..17773)
                     /gene="rplI"
                     /locus_tag="PKM23_02290"
     CDS             complement(17330..17773)
                     /gene="rplI"
                     /locus_tag="PKM23_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNY90332.1"
                     /translation="MKIILKKDIESLGAAGEIVTVKNGYARNYLIPGGFAAPATPGNM
                     RAVEIENKAAALRQQRGQREARDLADKLEKLSLTVSVQVGEEEKLFGSVTSQNIADLL
                     AENGFEIDKKKILLEDPIKALGVYDVPVKLHQDVTALVKVWVVRE"
     gene            complement(17788..18009)
                     /gene="rpsR"
                     /locus_tag="PKM23_02295"
     CDS             complement(17788..18009)
                     /gene="rpsR"
                     /locus_tag="PKM23_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNY90333.1"
                     /translation="MLRKKRICRFCEEKVDYIDYKDEKRLMRFTTEQGKIIPRRTSGT
                     CAKHQRQLVNAIKKAREIALIPYISEMTK"
     gene            complement(18039..18545)
                     /gene="ssb"
                     /locus_tag="PKM23_02300"
     CDS             complement(18039..18545)
                     /gene="ssb"
                     /locus_tag="PKM23_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNY90334.1"
                     /translation="MPDLKMPDINNVLIAGYLTSDPAFRKTSNGTPVINFYVASNRKY
                     RDNSGIWRENVCYVGVVAWHRLAEACAEILKKGSSVLIDGELQSRNWHNEDGTARNVV
                     ELRARRIQFLDRRSGRDVEELAQQSSDDTPGNPEEDGSKTKPAPEAQEFQKIEPTDFD
                     FGYQDLNL"
     gene            complement(18639..19109)
                     /gene="rpsF"
                     /locus_tag="PKM23_02305"
     CDS             complement(18639..19109)
                     /gene="rpsF"
                     /locus_tag="PKM23_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNY90335.1"
                     /translation="MRKYETIIVIDSLLKLEEIEGIINKYERFISANGGQIETVDRWG
                     KRRLAYEIKKRQYGFYVLIRFDAPPGMIKQLDREYRLNEYLLRTMTTLLNKRALKALA
                     KQYAAAPAPEPAAAPAPAPAAETPAAEAPAVEAPAAEAPAEKPKAAEESAPAAE"
     gene            complement(19160..19732)
                     /gene="pth"
                     /locus_tag="PKM23_02310"
     CDS             complement(19160..19732)
                     /gene="pth"
                     /locus_tag="PKM23_02310"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNY90336.1"
                     /translation="MQQKYLIAGLGNPGPRYANTRHNLGFLAADALADSLGAAFRKKN
                     GFSLLAEATCAGNELVIAKPQLFMNRSGEAIGQLVRYLNIEPERLLVVVDDADLPLGR
                     IRLRKQGSAGGHNGLKSVIDHLGSREFPRLRLGMGRPADAEGMINHVLSDFSREERVI
                     VKEIVQRAVETIHCFLQYGIDQAMNSCNTL"
     gene            complement(19762..20400)
                     /locus_tag="PKM23_02315"
     CDS             complement(19762..20400)
                     /locus_tag="PKM23_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNY90337.1"
                     /translation="MSAHVLELTLREKHGTLQAKKFRRNGEVPGIFYYHGKESIPFTV
                     SGKALQSTLATEATLLDVHFSNGDNRKCIVRDIQYHPINHAIIHIDFMGIKLDEKITV
                     SVPVHLTGISTGVKNDGGILQQVLREIEIECLPGDIPESIDLDITALAIGDALTVSNI
                     QTDKYVILGESDRTIVTITAPRAAEEEAVVEEAAAAEPELVGRKAEGAEEEK"
     gene            complement(20446..21387)
                     /locus_tag="PKM23_02320"
     CDS             complement(20446..21387)
                     /locus_tag="PKM23_02320"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNY90338.1"
                     /translation="MKIFSGRANQPLAQKIAGALKKNLGQISLKNFSDGEIWAKYEEN
                     IRGTDLFLIQPTHAPAENLLELLIMIDAARRASAKRITAVIPYFGYARQDRKDQPRVA
                     ISAKLVANLITTAGADRILTMDLHAPQIQGFFDIPLDHLYAAAVFIEHFRKMKSPELV
                     VASPDIGGIYLARAYARRLGTEMVLLEKRRPKHNIAEVSRLIGDVKDKDVLLVDDLVD
                     TAGTLIAGVQVLKEKGARNIYVACTHGILSGEAVQRIANSEIKNFFISDSVVVSKEKL
                     IDKIITLSVAELLADAIRRIHEDRSISDLFPEKGPKQ"
     gene            complement(21502..21573)
                     /locus_tag="PKM23_02325"
     tRNA            complement(21502..21573)
                     /locus_tag="PKM23_02325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21539..21541),aa:Gln,seq:ttg)
     gene            complement(21600..>22176)
                     /locus_tag="PKM23_02330"
     CDS             complement(21600..>22176)
                     /locus_tag="PKM23_02330"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNY90339.1"
                     /translation="AGLGGGSSDAAATLKALNREWGLHWPLERLLPLAAELGSDVPFF
                     LRGGCALGRGRGEILQPLAIPQGWWGVLVYPNLTISTRWVYENANFDLTKSLKNSKFY
                     SLTGFADKLSDWDIHLVNDLEPVVFNKYPYLKKLVDGFKRAGAFYARMSGSGSAIFGL
                     FLDKSDALSALAKTESSYTTFLFHPVAGPAL"
BASE COUNT         3996 a         6899 c         6855 g         4426 t
ORIGIN      
        1 tctctgtctt ccgctcaccg ataccgctct ggcgatcatc cgccgggtcc gccgcggcat
       61 ccatcctttc catgccgata aggagcatat ccatcaccgt ctggtgcgtc tcggcttgag
      121 ccagccgggg gccgctcttt tcatggtcgg tctgagcctg atcttcggca tcctggcctt
      181 tctgctcgcc cagggcatgc acacagattt caggtttttg ggacagctcc tgccctaatc
      241 acttgcaaat gtggccagaa actcctatct tctgccagag gttcaagctg gctaatacag
      301 gatggctcct acaaccctat gcgcacgcac cacgtccgaa attctccagt gcttccagct
      361 gccatttttc tggcagtttt ttttatttcc gcccacgcct ccgcacaatc ccgggcgggc
      421 ctcaccctgg tcagcagttc gcccctcgag aggatctacg agtggcaggc gcctgaatgg
      481 gaatggcaga gcgtgatcat cgacggcgag gcctgcctgc gccccggcgc ctctatcgcc
      541 acactctggt cacgccccgg cgatcccctg ctgccgatgg aggctctcca ttttgatctc
      601 ccaccaggcc agtctgctat catcaccctg accgattcac ttttcagcac ggaagcggcg
      661 cagctgccgc tctgtcccgc cccggtcatc gatccggaga gcgggaacct ggtctacagt
      721 atcaacgcca tgagtgaccg cagcggcctg gttccggagt cgagctggaa cagcaccatc
      781 ggcgaagccc gcggccggcg catcctggcg ttctccctcc atccggtgca gtacgatatg
      841 caacgcagag cattccgtct cctgcaatat gcccgtctga agatccggtt cacaatcccg
      901 gaatcctccc tgctgcgccc tgtcatccag ccgccgcaga cggcggcttt ttctcccctg
      961 acgaaaatag ctctggtcga tgaaggggtc tacgaggtga ccggcgccgc cctgcaggcc
     1021 gccggggtcg cgctgggcag cctttcgccg cagaactgcc agctctatca tcgcggagtc
     1081 gaacaggtga tcgaggtcga ggatggcggc gacggccgtt tcgatctcga ggatcgtctg
     1141 cttttttatg gccggccccg ccggggcgat gtcgaatttt acgatgcgta cagtgacacc
     1201 aatgtctatt ttctggcctc cggcagcatg ccggggcttc gtttcgcccc tgtggccgcg
     1261 ggctcttcca ttgcagcagc agccgacatc tttcccgaca ccctccattt agagcgagat
     1321 cttgcgtatt acgctggcga ttcggacaat gatatccata acagcgagcg cgtaccgggt
     1381 gagggatggg tctggagcgt tctcaacaag ggggggagtg cggccctggc tttcaccctg
     1441 actgccccgg ctgcagggca ggattccgcc cggctgcgca tgcggctgcg cggcaccacc
     1501 cgcgatgcca acggtccgga tcatcacgtt gtcctctctc tcaataacac ccagattcac
     1561 gaggcctggt tcaatgaccg ggaggagctg atcgtgcaga ctggcttcag ggccgacctg
     1621 ctgcgcgacg gcggcaacac cctcaccgtc aaactgctcg cggacggccc ctccgagcgc
     1681 tctcaggtct atgtcgactg gttcgaaatc gcctgtctgc gtacactggc tgcgagagag
     1741 gattggctca attttactcc cgacgccgca gcagaaaaac aggtattcgt tgcgggcttt
     1801 gccgattcca gcatcagggc gtgggatctc ggccggctgc gcgcctatct acccgaccac
     1861 gccggcaggc aatggcggga ccgcctttct gtgacctctg gtgggtacct ggacgggaat
     1921 tcggcccggc tcttccgcag cggcaaagaa gccggctatg gctatcgcgg ccacaacctg
     1981 tggcggatcg atccgcgcga cggccggctt gtcgagggca ggaattttga cacctacagc
     2041 gccaaagcac aggcggacag catggccgca tggattcagc gcctgcccgc agggacgctg
     2101 gtcgccgccg ccatccgcga tgagggcagc gtcagcatga atgaagcggc ccaccttgcg
     2161 ctcgaaagtt tgggaagtgc gctgacgcgc agcgtcggca ccagagattc gtgggcacta
     2221 ctcggccgca agggtgccgc tcccggttcg gcgctggagg ttttaaaaaa gagccagagc
     2281 ggacctgcgg tcatcgatac ggtgctcgtc ttcccggagg gagcctcctc tttcagcacc
     2341 ggcttgcacc tgcccgctga tgccggggga gaggtggtga tcttttcgga gaagggactg
     2401 aagagtccgc cgcgcctctc ccgctgcacg gagggagacc tgatcagcgg cggcgcaggc
     2461 gctgactata tcatcatcac gcaccccctc tttgaagcgg aagcccggca gctggcctcc
     2521 taccgcgccg cgcggaacgg ctggcgcacc cgggtcgtcc tggtcgaaga gatttatgat
     2581 gcgttcaacc atggcctggc ggatccatcc gcgattcgtg ccttcctccg ccatgtccgg
     2641 gagaactggc ccgaacccgc acccgcctat ttactgctct tcggcgatgc cagctgggat
     2701 cccaggaaca atatgaaggt ggcgcatccc acagaatttg tgcctaccta tggcaatccg
     2761 gtgagcgaca gctggttcgg ctgcctcgac ggcgagggcg atatcctgcc cgacctgcat
     2821 atcggccgcc tcccggtcca gaccccggag caggcccgga tcatcctgga aaagatcacc
     2881 gcctacgagg ccgccccctt ggcgcgctgg aaaaaggaat ttcttttcat cagcggcggt
     2941 ttcgattttc tcgaacagaa ccagttcggc cagcaatcgg cccagctgat caaacagtat
     3001 gtcactgctg cgcccacctt cggctccgcc accctcctga acaagacctc ggatggcctg
     3061 gaggagggcg aacaccgtca ggagataatc gataccatcg acagcggggt ggcctgggtc
     3121 aatttcatcg gccatgcggg cagccgaacc tgggacctga tgtttcacaa tgtggacatc
     3181 gaagccctga acaacgcgcc gcgttttcct ttcatcacct ccatgacctg tcatacgggg
     3241 cgttttgccg agcccaatca ggtggccttc ggcgagcact ttttgctggc cggcgaccgg
     3301 ggagcgatcg gattcatggg cacctcgggc tgggggtatt cctatgagga ttataccttc
     3361 ctgcgcaagc tttttcccgc cgccctgaaa gataccctcc gttctctctc cgaaatcatc
     3421 gatgcggcca aggtgcagtt atgggcggag gcgggaagca gcctgcagat ccgcgacatg
     3481 ctctaccagt ataatctgct cggtgatccg gggatcaagc tggctgtccc ggtcgtgccg
     3541 gatcttgcgg tgcagccggc ggacatcgag gtgatccccg agatgccgag cgaagcggat
     3601 tccaccgcca gaattaaggt gcgcgtgcac aatttcggcc tcggcacccg ggacagcgtg
     3661 gaggtgcggc tcctggctga tcatcccaat ctcggcgagc aggtgattgc gcccgcgatt
     3721 aaacttcccc cccttggccg ggtcgattcc ctcgaatgtc tctggccgct gcgcaacctc
     3781 gccggcgctg tcgatgtcat cgcccggctc gatcccgccg acaggttcct ggaggccgac
     3841 gaggaaaaca atcaggccag tcgctcggtc acggtcctgt ccagtaaaat tcagctcctt
     3901 ctgccctctc cattcgcagt gcttccagcc gccgatgcca tggtccggct ggacgggagc
     3961 aatctcggcc tgaaagggga ggctatctac cgcatccaga tcgatacctc cctgaccttc
     4021 gccagcagcc tcctgatggc ggattcgatc gtcgcctcat cggcccccgc attcgactgg
     4081 cgcccgccaa acctgcagcg ggaccagctc tatttcctga gaatcagcaa ttcatccaac
     4141 gagccgggat attggcaggt gagcagcttc aagacctccg tcctggctga gaacggctgg
     4201 cggcagcagg gaaattacgc ctttgctcag aacgggcggg acaatgtgga atcggcggga
     4261 acagccgctc tcgccttgct cccactcccg ctttatgccg aatccgcggg ctatgcagac
     4321 ggcaatttca cccgtatact cgtcggagat gcccccgcca ttcaaccgcg gcggggtcat
     4381 aatctggttg ttgtcgatcc cgggactttc aaggtggagg cgacgcggaa ttttgatacc
     4441 tatgcggaca gctccgcggc caaccagatg gcggccctga tcgaggggct cgttgccggg
     4501 aaaatagtcc tggcggggat catggatgag gggaccgtca acatgacaga acgggccttc
     4561 cgcgcgctgg agagcatcgg cagcgcccag tgccgcagcg tcggcgtccg ctcctcctgg
     4621 gcgatcatcg gccgaaaagg cgccgctata ggcagcgtac ccgaaaaaac cgtaccgccc
     4681 aatgccggct ccgctgcggt cgtggatagt ctttattatt atcccccccg cggcaccctc
     4741 acctcccccc gcatcgggcc ggcgacggcc tggcgctcgg tccgcgccga agcggaggtg
     4801 ccgcagcagt gcagcctcac cctcagtctg ctcggccagc gccgcagcag cgccctctgg
     4861 gacaccctgc gcaccggcct gcccgtcaat ttcgacaacg acatctcggc gatcagcgcg
     4921 cgggagtacc cctggctgca gctggtggcc gacttttcca ccgctgacca gctcagcacg
     4981 ccacgcctgc acgcctggca ggtgctccac gaccccgctt ccgacctggc ggtgagcccc
     5041 gccttcctga gcctctcggc cgattcggtg ctcgccggac agccggtggt cctcaaactc
     5101 gacatcttca atatcggact cgcgagggcg gacagcgtgc gcattacctt cagcgaatcc
     5161 gccccgggca gcgacgagaa actcttctcg cgcgtcaccc tgcccgggag cctggccgcc
     5221 gaccagaaac tgaccatcga tcagctctac acccccgccg gcaagccggg cagccgcctg
     5281 ttgacgatcc gggtcgacgg cgacaaccgc atcaacgagc tctccgagtc caataatacc
     5341 gtcaccaccc gcgtgcaggt ggtggccgac accctgcagc cgcaaatcga aatcaccttc
     5401 gacggccgca ccatcacccc cggcgactgg gtggccgcgc agcccctcat ccgggcgcgc
     5461 ctgatcgatg acagcccact ctcagtcgcc gacaccctgc aaatcaacct cctcatcgat
     5521 ggcctgcgcg tgcccttcag cggcgaaaac ggggtgcagc tgctcgcccc gcctgatacc
     5581 ctggcgatcg ccctcctcca gcatcggccc ctgctccgcg cgggggagca cagcctggag
     5641 attctgttta ccgacgccag cggcaacatc aacaataccc gcatcgattt ttccgtcgct
     5701 gccggcctgg agctgcagcg ggtgatgaac tatcccaatc ccctctccga ccacaccgat
     5761 ttcacctttt tgctcacccg tcccgccgag gtgcgcatcc gcatctatac ggtcaacggc
     5821 cgcctcatcc gcctgctcga ggcgggttca ctcggcgcgg gctttaaccg cctcttctgg
     5881 gacggccgcg acggcgacgg cgacctcatt gccaacgggg tctatctcta ccgcgtcgag
     5941 gcccgcagcg gcagcgagca cgccgaggtg gtcgaaaagt gcatcatcat gcgctgagtc
     6001 cgcccttgcc tggcctcctc cttttctaaa aaatgacgtt tgaattttca gttggttttc
     6061 gtacatttca ggctgaattt tccccgggct gctgcatccg ctcacacgag atacggctgc
     6121 acggaattat atcccttgcc acgaggagct gcttgcgcta tctgacttat ctgacggcct
     6181 taaccgctgc actggcggcc tccctggtcc tggtgcgggc cgccatggcc ctggcggtca
     6241 catgggacat ccaggaccat ccgatcggcc gcaaggacca cgcccgggtc acgcccctgc
     6301 tcggggggct cggcatctac gccgcctttc tgctcgcctt ggccgccggc ctcggactgc
     6361 tctggtggtg cggccgctcg ccgcaggctg ccgcccaatt cccgatgctg gcgcgccagg
     6421 ccggactgct cggcggggtg ctgcccaagc tggcgatgat catggcgggt gccaccctcc
     6481 tggtcgtggt cggcctcatc gacgacatcc gcggcattac ctttccgccc gcgatcaagt
     6541 tcgtcgccca actcgccgcc gcggttatcg cagtggccgg cggggcgcgc agcagctttc
     6601 tccccctctt ctggctggat atcctttttt cgattctgtg gatcgccgcc atcagcaatg
     6661 tcttcaactt tctcgacaac atggacggcc tctcggccgg catcgccctg atctgcggcc
     6721 tgctcttctt ctggctcacc gccagccagg ggcagtattt ttccgccctg ctcttcgccc
     6781 tgctctgcgg cgccctgctc ggctttttgc gctacaacat ccacccggcg cgggtcttca
     6841 tgggcgacgc cggcagcctc ttcatcggct atctcttcgg agcgctgagc ctgaccagct
     6901 cctacgtcgt cgctggcagc gcctcgctca tcccggccat catccccgtc attgtccttg
     6961 gtgtgcccat ctatgatgcc gtcacggtga ttctcatccg cattcgcgag catcgtccgg
     7021 tatatgtagg ggatcaatgt cacatctccc accgcctgct caggctcgga ctttcgcgca
     7081 accaggcggt ggtttttgtc cttctcatct gcatcgccat cggcatgagc gccctgctgc
     7141 tgccctacct cagcctgcgc ttgagcatca tggtgctgct gcaaaccatt ctgatctacg
     7201 tcatcctgac cctgctcatc cgggccggca ggaaataact acaaggagtt cccatgaaag
     7261 cactgataac cggcggggcc ggctttatcg gctcccatct ggccgaacgg ctgctggagc
     7321 gcggtgatga ggtctgcgtc atcgatgacc tttccaccgg caaactcgaa aatgtggccc
     7381 atctgcgcga caatccgcgc ttccatctcg ccgtggagac cattctcaac gaaaccgtga
     7441 tggaccgcct ggtgagcgag tgcgacgtca tttaccatct tgccgcggcc gtgggggtcg
     7501 agctgatcgt caagagtccg gtcgagacca tcgagcgcaa cgtgctcggc agccatgttg
     7561 tgctgcgcct cgcctcgcgc tatctgcgca aggtgctgat cacctcgacc tcggagattt
     7621 atggcaagag cgattccatc cccttcagtg aggaggacga ccgcgtgctg ggggcgacga
     7681 ccaagagccg ctggagctat tcctgctcca aggcgatcga cgagtttctc gcccttgcct
     7741 accacaagga gaagggggtg ccgacggtga tcatgcgcct tttcaacacc gtcgggccgc
     7801 gccagaccgg gcgctatggc atggtcatcc cccgcctggt cacccaggcc ctggcgggcc
     7861 gcccgctgac ggtatatggc gacggccagc aggtgcgctg cttcaccttt gtcgaggatg
     7921 tggtctgggc ggcgatccgg ctgatggagg agcccgctgc ggtggggcag gtcttcaatg
     7981 tcggcaacga caaggggatc agcatcgccg atctcgcccg caagatcgtc gagatgaccg
     8041 gctccaaatc ggagatcagc tttgttccct actcggaggc ctacgaggag ggctttgagg
     8101 atatgcgcat ccgcgtgccc aatctcaaca aggtaagaga gaccatcggc tatgagccca
     8161 gggtgcagct ggatgagatc ttgcggcgcg taattgaaca ctttttagct aaaaaaggtc
     8221 aggattaacg aatttgtccc ctcctgtgcg gtggccgcgg ccatctccgc ccggggagtc
     8281 aggaaatctg ggtcatgcac gggacagatg tggataactt ccttattttt gttcaattat
     8341 tgcgcagcag aggggaaggt ggggcggatg gttccacacc gtcggctgcc ggctcttccc
     8401 gggctttctc cgccccattt catggttttt caatgaatta ggcggacgat acaagattaa
     8461 ttaaaacaat atattggaca ggtgttccag ctccgtcctt tgcccccgct gccggggcgc
     8521 ggaggcgcgc ggttttccga tcggggacgg gtagcccctc gccgccagcc ggcggccaga
     8581 tgagggcggg catttgtgca aaagatgtgg ataacttgtg ggcaaagtgt ttttagtccg
     8641 gccgggcgcg cccaccacaa gatataggcg cccctgcccg cgcctcacca caaataacca
     8701 ttgcttttct gccctgaatt tagtaaataa tcagtgaaac gagttccggc ggcccgtccg
     8761 cgccgtccgg ccatccatca gcccgggagt agatgaccca cgacctgaca aatcttcttt
     8821 tgctgctcgc ggccctgatc gtgctcctgg ccctttccga gctgctgcgc cgccgcttcc
     8881 acggcaccga ggagttttcg cgcaagttcg tgcacatgtt cacgggcgtc ctggtggccg
     8941 tctcgccgtt ctttctcaaa agccgctggc cgatcatcgc cgtggccctg atttttgcgg
     9001 cgttcgatta ttactccgtg cgcaggggct ggctcaaggg gatgcacggc ctgcaccgcc
     9061 gcacctacgg cacggtctac tatccgatct ccttcgtgat tctggccctg ctcttctggg
     9121 agagccatcc cctcatcctc atcaccgcca tgctgctgct ggcgatcagc gacgccgccg
     9181 ccgccctggt gggcgagaat ctctccgctc cccgcatcta ccatatcggc ccggataaaa
     9241 aatccgttga gggttcgacg gcgatgttcc ttaccagcct ggcgatcgtc gccgtcgtgc
     9301 tgctcctctc cggccggccg gccatggagg tgctctggtt ctccgccctg gccgctttct
     9361 tcgccacggt ctgcgaggcc ctctcctatc agggttcgga caacctgacc gtgcccctgg
     9421 gcgccgccct ggtcctccac tatctgctca cccactccca aaccgatgca gcgattttta
     9481 ccctcggggt ggccatggcg gcagctgtcg ccctgctctc cttcagactc aagtttctct
     9541 ccgcgagcgg ctcggcggcg accttcatcc tcggcaccat cgttttcggc atcggccgct
     9601 ggccctacac cctgcccatc ctgaccttct tcatcctctc cagcctgctc tccaagatgg
     9661 gcaaaaagcg caagtagaaa ctggcgggaa tgatcgagaa agggggggcg cgcgatgcct
     9721 ggcaggtgat cgccaacggc gggccgggcg ggctgatcgt gattctcgcc tacatctggc
     9781 cccatccggt ctggtttctc ctctatgcgg cctccctggc ggcagcgacg gctgacacct
     9841 ggggcaccga gatcggcacc ctgtcgcgca ccgatccgcg ctcgatcgtc accttcaagc
     9901 cggtgccggc cgggacctca ggcggcattt cgctcatcgg caccctcggc tcggccctgg
     9961 gcagcctgat tctggccggg gtgagctggc tggtcacgcc gcagaactct ccggctgtgg
    10021 tcggtttgcg cgaattcatc atcatcttcg ccgccggggt ggcggccagc ctctttgaca
    10081 gcctgctcgg ggcgaccctg caggccaaat tccgctgccc ggtctgcagc aaggtgactg
    10141 aaaagagcca tcactgcctc gggaagaaga ccgaatttgt ggaggggatc gcctggatga
    10201 acaatgaccg cgtcaacatg gccgctgcgg cctttgcgat tattctggta tatttttcaa
    10261 gtatttggct ggtttaattt cacacagagt cacaaagaca cagggagacg aatcgttccg
    10321 ttatacgctc tcctctgtgg ctccgtgaga ctctcttttc gatatcattg aattgaacaa
    10381 ggaggatgca tgaccaccat tatcgatgtc ttcgcacgtg aaatactcga ctcccgcggc
    10441 aaccccacca ttgaagtgga tgtggccctc gagggcggcg ccatggggcg cgcagcggtt
    10501 ccctccggcg catccaccgg cgaacatgaa gcagtcgagc tgcgcgacgg cgatgccgcc
    10561 cgctataaag gcaagggcgt gctccaggcg gtgaagaatg tcaatgatat catcgccgac
    10621 gaggtgatcg gcgaagacgc caccgagcag accctgatcg accggatcct catcgagctg
    10681 gacggcaccc ccaacaaggc caaactgggc gccaacgcca ccctggcggt ctccctggcc
    10741 acggccaagg ctgcggccga ggccttcggc ctgcccctct atcaatatat cggcggcagc
    10801 aacgccaagg tcctgccggt gccgatgatg aatatcctca acggcggcat gcacgccgac
    10861 aacaacgtcg acctgcagga gttcatgatc atgccggccg gcgccccgaa tttttccgag
    10921 gccctgcgca tgggcgtcga gaccttccac agcctcaagg gtgtgctcaa gaaaaagggc
    10981 tacaacaccg ccgtcggcga cgagggcggc ttcgcccccg acctcaagtc caatgaagag
    11041 gccctgcagg tgatcatgga ggcgatcgag aaggcgggct acaaggcggg caaggagatt
    11101 tttatcgccc tcgatccagc cgccagctcc ttctacctca ccgacaaaaa ggtctacaac
    11161 ctcgcctccg agaagaagaa actgagctcc gaagagatgg tcgactatta cgccaatctg
    11221 gtggagaagt atcccatcac ctcgatcgag gacggcatgg ccgaggacga ctgggacggc
    11281 tggaagctga tgaccaagaa gctcggcaaa aagattcagc tcgtcgggga tgatctcttt
    11341 gtcaccaata ccaaacgcct gaaaaagggc atcgatctgg gcgtctgcaa ttcgatcctg
    11401 atcaaactga accagatcgg caccctgacc gagaccctgg atgcgatcga gatggccaag
    11461 accgccagct acaccgccgt ggtttcgcac cgctcgggcg agaccgagga cagcaccatc
    11521 gccgatgtgg tggtggccac caatgccggc cagatcaaga ccggttcggc ctgccgcacc
    11581 gaccgcatct gcaagtacaa ccagctcctg cgtatcgagg agaatctcgg tgaggtgggt
    11641 cattatgccg gcatgggcgt gatcaagcgc aaataggctg taatttgaca ccctgccggc
    11701 cgccggccgg caggggctct tctggagagg ggttgatgat gaacgcaaaa agagggacgt
    11761 tcgcaggctt caagcggcgt ctggggaaga tcaaggtcac gcggggcaga ttttatctga
    11821 tcggcttcgt gatcctcttt ttcggctatg tcgccggctc ttccggattt gtcgcccaga
    11881 tccggctgtg gcaggaggga cgccagcttc agaaggcgat cgagctggag cagaagaaaa
    11941 agaagtggat gcaaaaagag gccgagagcc tgaccaatga ccggaatcgc atcaagcgcg
    12001 aagcccagaa agagcacagc ctcggcgagc cggatgagat catcgttcac gtgcagtaac
    12061 tcccgtcgtg gagcacttta tcaaccgttg atcgaaagat gccatcgcgt cgctgcgcag
    12121 atggcatttt ttgttgtttt ctgttgtttt ctcttttacg ggcgggccgc agcccagggg
    12181 atggcggccg ccggcgaggg ccccggcctc aatatgccgc tgtccctgcg ctacaggcag
    12241 gagttgaaca accatctctg gcagttcgac gcgggacggg tcgttgtcct gggccggcac
    12301 tgggaatgga gtttccagga gcagttccgc acctcgatgc tgcggctcgg cggcagcgag
    12361 gacaaatgga aggacgacca ctccttttcg accgcgctgg gttttctgct gctgccctcc
    12421 ctgcgggtgc agacgctttt ccgctctttg tcattttttg accggcagac aggcttgaac
    12481 aacgacgtgc gcagtaatgc cgtgggattg ggagcgctct acaccccgac accgcgcctg
    12541 cgtttggccg gccgggccgg gcccaagtgg gagtcgcgga tgggcttccg cgaccggggc
    12601 ttcttcctcg ccggggaggg ggcggtttcc ggcctcgact ggggcggcta tctcaaccac
    12661 ctccagggcc tggtggagat ggaccgcttc agtcagcgca aaaaccatga ggccaatgtc
    12721 gcctatgccg tggcgcgggt cttcagccag gccagcagcg attccctgcg tctctacacc
    12781 ggcagccggc ggcgggacaa ctatacctcc tggctcggcg atgtcgagag cctgcgcgaa
    12841 ggcaacaagg gctttgagaa catcctcacc tatggcctgg gcgactggtc gcgcctgctc
    12901 ctgcgcacca gctggcagtt ccgcaatgtc gagctgctct ccttcgggat ggaggagttg
    12961 gcgcggcagc gcaagcgcaa cgaccgcctc ggtgatcacg cccttgaact ggcagcggcg
    13021 cggaaccgca tgcaggggcg gatggccctg agctacaaca cccaacgcca gaaatacgac
    13081 attgccgcca gcggcgcgca gtcgcccttt tccaagtaca cggcctttgt cacgccggac
    13141 aacagcagca gccggctgca gctctccggc gagctcagca ccccggtcgg ccgccgcgac
    13201 agcctttacg cctatgcctc ggtcagccgg tttcagtacg acacgccgga caccaacaat
    13261 tttgacgacc gcgacgaact gcgcatcaac tcccagttca tcttcggccg tcagatccgg
    13321 cccgaactgc gcctcgaggt gcaggcgagt gtcagcctct atcacatggt ctacatcttt
    13381 ggcgaacgca gcgccgataa cagctggaac cgcatcctgc ggctgcggcc ggtgctgcgc
    13441 tacgccccat cgcagcggct gcagtggcac aacgcctttg aggtgcttgc caactatgtg
    13501 gactatgact ttgacaatct ggccctgcag acccggagtt ttgtcttccg caaatttgcc
    13561 tgggacgatt ccctccggct ggaggtgacc cggcgcagca gcctcaggct cgactatcgt
    13621 ctgcagctgg aggagaacgg ccagctctac tgggaggcct ggaaggagcg cctcctgacc
    13681 acacgcacca gccactggct gcagctgcgc gtgctctata ccgcggcccc ctcgctctca
    13741 ctgggccccg gctacggttt ttacagccgc ctggagtggc ggcacgacat cgatgccctc
    13801 ggccgcgagc gggtcgcctg cgcccagagc ttcaacagcc atgggccggc cctgagcatc
    13861 tattacacgc cctcgccgcg cacccggttt gttctcgacg cccacaggcg ccgggtcaag
    13921 ccctggcagc aggagccgta taccatcaat accctggatg tccgcctgga ctggcttttc
    13981 taggcgcacg acgcacctcc cacaggcgca ggagggaggg ccggacctga tgcagggaag
    14041 gaaaaggatt gagcgaagca aagcttgaaa tgcacgagat gatcatcccg agtctgccgg
    14101 agcagatcca gcgcgtcgag gaaaagtgcg agcagatggc cggcctggcg ggcttcgccg
    14161 acgacgaacg ggatgcgatc gccattgcca tcaccgagat ggtcgccaat gcgatctatc
    14221 acggcaacaa gagcaatccg aaaaagagcg tgcgcatccg tttctatctt cagaccgggg
    14281 gattgaccgt gcacatcacc gatcagggtg agggctttga tcccaaggcg gtccccaatc
    14341 ccctgctgcc cgagaacctg ctcaaggaca acggccgcgg catcttcatc gtgcgctcga
    14401 tgatggacag cgtcgagtac cgctttgagc ccgggggggg aacgacggtc atcctgacca
    14461 aatgcaaaaa aagcgatgtg cgggattagc cgggagcccc tgcaagccgg ggtgcgccgc
    14521 catccctgcg ccgccctcgc cctctctctg ttcctcgtcg cgctctgctt cactcccgcg
    14581 ccggccgcga ctccgcccca gtttcccgat tccaccctca tcgaatcgat cctttttgtc
    14641 ggcaacgacc gcacccgcga ggagatcctg acccgcgaga tgcagctgcg cgagggggat
    14701 cccttcgatc ctcgcatcgc ggaggaggac cgccagcgca tcgagaacct cggcctcttc
    14761 acccgcgtcg agcttgaact tctccccggc gccggcgggg tcatcctgct ctacatcctc
    14821 agtgaacgct ggtacctctt tccctatccc ctgcttttca tacacgaccg cgactggagc
    14881 aagctctcgt acggggccgc cctgcgtcat gacaatttcc gcggccgcgc tgttgtcgtc
    14941 aataccaatt tctggttcgg ttacaatccg agcgcaagcc tgagctacac caatccctgg
    15001 atccacagcc gccaccggct ggccttcacg gcgcagtttg tttacaaccg cgtcgagaac
    15061 ctcagtccca acctgagcga tttcacagaa aagcatcttg gcgcggctgt ggggttgagc
    15121 aagcgcctgg gcagcgatac gcgcgtgggt gcgggggtgg gctatcgcga gatccatctc
    15181 cccccggaac tcggcctcac ccgttcggcc ggcggcaccg accgcatcgg ccagctctcg
    15241 gcctcgatca actatgacag ccgcgactac tctccctggc ccggccgggg ctggtatgtc
    15301 gatgccgggg tggcgcacaa gctggtcttg tccggatcgg actatctctt cgccatcggt
    15361 gatgtgcgcc gctaccagcc cctgcccggc ggtggaaccc tggccggccg cttccgcatc
    15421 gactggagca gcggtcgggt gccgctctac aacctccttt acctcggcta ctccgagcgc
    15481 gtccgcggcc atttccgcga ggtggtggag ggcgacggcc ggatggtggc cggggcggag
    15541 ctgcgctggc cgctgctcaa acggcgcttt tttaatcttg ccggcgcgga tcagggtgat
    15601 ggctatgcca attatttccg caatctcccc ttcgccctgc acgcgaccct tttttacgat
    15661 gccgggctca tcgccgacgg cggctggtcg cgggcggaac agcggcgcct gggcgggttc
    15721 ggcgcgggac tggcggtcca gctgccctat gtggagctgc tccgtttcga gcgcgcctgg
    15781 gatctggcgg ggcgcggtga atacatcatc gacatgaagg tctggtttta gccatggcgc
    15841 atattcattt tttcttcgtc gatcccgccg atgtcgggcc cgggcagctg gtgctgcgcg
    15901 gggcggaata caatcatgcg gcgcgcgtgc tgcgcaagca ggcgggggag cgcatcatcg
    15961 cggtggacgg ctgcggccgg cgctacgagg gcccgatcgt ggccctgggc agtggggagc
    16021 tgacggtggc gatcgaatcg gccgtcgagg gcggaggcga gccgcggctg cggctgaccc
    16081 tggcccaggg gctgctcaag ggggggatgt tcgactgggt ggtggaaaag gggacggaga
    16141 tcggcatttc agtttttcag ccgatgatca ccgctcatac ggtggtggag gggcgcggcc
    16201 gcgaggggcg ctggcaggag aaggcgcggg cggcgatgaa gcagtgcggc cgcagcctgg
    16261 ccccggtggt gcgccctgtg gccggtttcg cccgggcgct cgaggccgcc gggggggagg
    16321 cgctcttcat cgcctgggaa gaggccgagg ttgcgccggc gggactggcg gaagccctgg
    16381 cggggcgcac ggcggcgacc cttttcatcg gtcccgaggg gggattcagc gaggaggagg
    16441 tggccctggc ctccgcggcc ggggcgcgga cgatcagcct cgggccgcga cggctgcgca
    16501 gcgagaccgc ggccctcacc gcggcggtgc ggctcctcga tgccgcgggt gaactggcct
    16561 agggcgttga actgcagcgt atgggctttg ttcagcgggc gcgcacgtac caggcgaaga
    16621 gggcgcagaa gaaggagatg gccgccgaga cggccagttc cgcatggcgg tagcgcgggc
    16681 tgcggcccgg agccggcgag ggcagcatga gggcggagag cagggtcagg acgaggagat
    16741 tataccagcc gccgaggatg agcccgaggg tggcgagcag gcgggagagg atgccgggca
    16801 ggcggtggcg aaaggccgga aaggcgtggc ccttgaggcg cgcggttttg ccggcgaggc
    16861 cgagctggag gtagtggatg atgggggggg ctgcgaagcc ggcgatgacg agggcggtca
    16921 gggtggtggt cagccaggac gagagccagt aggaatcctg cggcagggtg gcctgcaaat
    16981 accaggcccc cccgagcagg gatatgagat gcagcccctg gtcgataaaa aaatagccca
    17041 gtccgtccct ggcatggaaa agcgcaagaa agagcttggt ccggtccagg gtgaaatgga
    17101 caagggcgat aaagaggatg accagccaga cagcgggccg gtccagcagg gggaaaaaga
    17161 gcgcgacgtt caccagagaa cagatcagga catgaagcgg gaccccccag gccgactttt
    17221 gcttgaggcg gaagaggctg tcggtctgca gcgggaaatc accgatgaca tgggccagca
    17281 gcagcagttg aaaataatcc attcccggct ccgcggagat ggcggcggac tattcgcgaa
    17341 caacccacac tttgaccagc gcggtcacat cctggtgcag cttgacgggg acgtcataga
    17401 cgcccagggc cttgatcgga tcctcgagga ggatcttttt cttgtcgatc tcaaagccgt
    17461 tttcggcgag cagatcggcg atgttctggc tggtcaccga cccgaagagc ttctcttctt
    17521 cacccacctg caccgaaacc gtcagtgaaa gtttctcgag tttgtcagcc aggtccctgg
    17581 cctcgcgctg tccgcgctgc tggcgcaggg cggccgcctt gttttcgatc tcgacggcgc
    17641 gcatgttgcc gggggtggcc ggagcggcaa agccaccggg gatgagatag ttgcgggcat
    17701 agccattctt gaccgtgacg atctcaccgg cggcgccgag ggattcgata tcttttttca
    17761 ggataatctt catggggatt ttccagacta tttggtcatt tcagagatgt agggaattag
    17821 ggcgatctcg cgtgcctttt tgatcgcgtt gacgagctga cgctgatgct tggcgcacgt
    17881 tcccgaggtg cggcggggga tgatcttgcc ctgctcggtg gtgaaacgca tcagccgctt
    17941 ttcgtccttg taatcaatat aatccacttt ttcttcacaa aagcggcaaa ttcgtttttt
    18001 tctcaacatg ttgactcctt ttatggccta gagaggggtt acaggttcaa atcctgatag
    18061 ccgaaatcga aatcggtcgg ttcgatcttt tggaattcct gcgcctcggg cgcgggcttg
    18121 gttttgctgc cgtcctcctc ggggttaccg ggggtatcgt cgctgctctg ctgggcgagc
    18181 tcttcgacat cccggccgct ccggcggtcg aggaactgga tgcgccgggc gcgcagttca
    18241 accacattgc gcgcggtgcc gtcctcgtta tgccagttgc ggctctgcag ctcgccgtcg
    18301 atgaggactg acgagccctt tttcaggatt tcggcgcaag cttcggccag gcggtgccag
    18361 gcgacgacac ccacatagca cacgttttcc ctccagatgc ccgagttgtc gcggtacttg
    18421 cgattggagg cgacgtaaaa gttgatgacg ggtgtgccat tggatgtttt ccggaacgcc
    18481 ggatcgctgg tcaaatagcc ggcgatgagg acgttgttga tatccggcat cttcagatct
    18541 ggcatatcgt acactcctca agccccaacc ggcgggcttg aaatggacag aggtctgact
    18601 cacgcgagcg tgagtatgct aaagaacgga atccgcacct attcggccgc gggggcgctc
    18661 tcttcagccg ccttgggctt ctcggcgggg gcttccgctg cgggagcttc cacagctggg
    18721 gcttccgcag caggggtttc ggcggctggg gccggggccg gagctgcggc cggttcggga
    18781 gccggggcgg cggcatactg cttggcaagg gccttgaggg cgcgcttgtt gagcagggtc
    18841 gtcatggtgc ggagcagata ctcattgaga cgatattcgc ggtcgagctg cttgatcatc
    18901 cccggggggg catcgaagcg gatcaggaca taaaagccgt actggcgttt tttgatctcg
    18961 taggccaggc ggcgcttgcc ccagcggtcg accgtctcaa tctggccgcc gttggcggag
    19021 atgaagcgtt cgtatttatt gatgatgcct tcgatctcct cgagcttgag cagggagtcg
    19081 atgacaatga tggtctcata tttacgcaaa ctagtcctcc ttcaggtagt gcactgcgtt
    19141 ggtgatatcg gttgactgat cacagggtat tgcagctgtt catggcctga tcgatgccgt
    19201 actgcaggaa gcagtggatg gtctcgaccg cgcgctgcac aatttccttg acgataacgc
    19261 gctcttcgcg gctgaaatcg gagagcacgt ggttgatcat cccctcggca tccgcgggcc
    19321 ggcccatgcc caggcgcagg cgggggaact cgcgggagcc gagatggtcg atgaccgatt
    19381 tgagcccgtt atgaccgccg gcgctgccct gcttgcgcag gcggatccgg cccaggggca
    19441 gatcggcgtc atcgacgacg acgaggagcc tttccggctc gatgttgaga tagcgaacga
    19501 gctgaccgat ggcttcgccg ctgcggttca taaagagctg gggtttggca atgacgagct
    19561 cgttccctgc gcaagtggct tcggcgagca gggagaagcc gttttttttt ctaaaggcgg
    19621 cgccgaggga gtcggcgagg gcatcggcgg ccagaaagcc caggttatgc cgcgtattgg
    19681 cgtagcgggg gcccgggttg cccaatccgg caatcagata tttttgttgc atggccggtg
    19741 gctgtccgtg gcagaagagg attacttctc ttcttccgca ccttcagcct tgcgtccgac
    19801 cagttcgggt tcggcagcag ccgcttcctc gacgacggcc tcttcctcag ccgcgcgcgg
    19861 ggcggtgatg gtgacgatgg tgcggtcgga ttcgccgagg atgacgtatt tgtcggtctg
    19921 gatattgctg acggtgagag cgtcgccgat ggcgagcgcg gtgatatcca ggtcgatcga
    19981 ttcggggata tcgccgggca gacattcgat ttcgatctcg cgcagcacct gctggaggat
    20041 gccgccgtcg ttcttgaccc cggtcgagat gccggtgagg tggacgggca cggagacggt
    20101 gattttttca tccagtttga tgcccatgaa atcgatatgg ataatggcat ggttgatggg
    20161 atggtactgg atgtcgcgga cgatgcattt gcggttgtcg ccattgctga aatggacgtc
    20221 gagcagggta gcctcggtgg cgagggtgct ctgcagggcc ttgccgctca ccgtgaaggg
    20281 gatggactcc ttgccgtgat agtagaatat gccgggcacc tcgccgttgc ggcggaattt
    20341 tttcgcttgc agggtgccgt gcttctcgcg cagggtcagt tcaagaacat gtgcagacat
    20401 tatggttctc cagtctttgg ctcaatgcca gggctaacga tttgcttact gtttcggtcc
    20461 cttttccgga aagaggtcgg aaatggaacg atcttcatgg atgcggcgga tggcgtcggc
    20521 gagcaattcg gcgacgctga gggtgatgat tttgtcgatc agtttctcct tgctgacaac
    20581 gaccgagtcg gagatgaaga aattcttgat ctctgagttg gcgatcctct gcaccgcctc
    20641 gccggagagg ataccgtggg tgcaggcgac gtagatgttg cgggcgccct tctctttgag
    20701 cacctggacc ccggcgatga gggtgccggc ggtgtcgacc aggtcgtcga cgaggaggac
    20761 atccttgtcc ttgacgtcgc cgatgaggcg gctgacctcg gcgatgttgt gtttcgggcg
    20821 ccgtttttcg agcagcacca tctcggtgcc caggcggcgg gcataggcgc gggcgagata
    20881 gataccgccg atatcgggtg atgcgaccac cagctcgggc gatttcatct tgcggaagtg
    20941 ctcgatgaag acggcggcgg cgtaaagatg atccagcggg atgtcgaaaa agccctggat
    21001 ctggggcgca tgcaggtcca tggtcaggat gcggtccgcg ccggcggtgg tgatgagatt
    21061 ggcgaccagt ttggcggaga tggccacgcg cggctgatcc ttgcggtcct ggcgggcata
    21121 gccgaaatag gggatgaccg cggtgatgcg tttggccgag gcgcggcgtg cggcatcgat
    21181 catgatcagc agttcgagca gattctccgc cggggcgtgg gtgggctgga tgaggaagag
    21241 atcggtgccg cggatgttct cctcatattt tgcccagatc tcgccgtcgg agaaattttt
    21301 cagtgaaatc tgcccgaggt tctttttcag ggctccggct atcttttggg cgaggggctg
    21361 gttcgcccgg ccggagaaaa tcttcatctc gcgatcttgc gccattggga cctgtatcga
    21421 tcttgtttcg tcagaaggga ggaaataaaa aaaggacgaa aatccaggca gactttcgcc
    21481 attccttccc ttttcacagg gctggggcgg gaggattcga acctccgaat gcgggatcca
    21541 aaatcccgtg tcttaccgct tgacgacgcc ccagagagtg atgcgggtgg ggatgcgcct
    21601 cagagggcgg ggcccgccac cggatgaaaa agaaacgtgg tgtaggagct ctccgtcttc
    21661 gccagggcgc tcaaggcgtc gcttttgtcc aggaacaggc cgaaaatagc cgaaccgctg
    21721 ccggacatgc gagcgtagaa tgccccggcc cgcttgaacc cgtccaccag ttttttcaaa
    21781 tacggatatt tattgaaaac aacaggttcg agatcgttaa ccaggtggat atcccagtcg
    21841 gaaagtttat cggcaaaacc ggtcaagctg taaaatttac tgttttttaa cgattttgtc
    21901 aagtcaaaat tcgcgttttc atagacccag cgggtggaaa tcgtcagatt ggggtagacc
    21961 agcacccccc accagccctg ggggatggcc aggggctgga ggatttcacc gcggccgcgg
    22021 ccgagggcgc agccgccacg caggaaaaag gggacatccg agcccagctc agccgccagg
    22081 ggcagcaggc gttcgagggg ccagtggagc ccccactccc ggttgagggc cttgagagtc
    22141 gcggcggcgt cgctgctgcc cccgcccagg cccgcg
//
LOCUS       DAOPWU010000020        21608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_2420_length_21608_cov_5.107734, whole genome shotgun sequence.
ACCESSION   DAOPWU010000020 DAOPWU010000000
VERSION     DAOPWU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21608
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2420_length_21608_cov_5.107734"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PKM23_02335"
     CDS             complement(<1..278)
                     /locus_tag="PKM23_02335"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNY90340.1"
                     /translation="MKNLKLIHAAGLLLALVLPLRGADPDSAALKLHRELLTIDTHCD
                     TPLHMLDTTWDIGAVHPSGTRDRGKIDLTRMENGNLDALFFAAFVSQRA"
     gene            complement(401..1708)
                     /gene="miaB"
                     /locus_tag="PKM23_02340"
     CDS             complement(401..1708)
                     /gene="miaB"
                     /locus_tag="PKM23_02340"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNY90341.1"
                     /translation="MKVYIETYGCQMNEYDSEIIRAILDEADLESTDAPEQADVVLIN
                     TCAVREHAALRVVNRIHEIRRLPNGARLRLGVVGCMATGLKKQLGGNRRLPVDFFAGP
                     DSYRRLPGLITTAAAEKKELYAAELSDFETYADIYPLRRGGVNAWIAVMRGCNNFCSF
                     CIVPFARGRERSRSPRSITDEVRRLAADGFCQVTLLGQNVNSYRYEQHDFAALLREVS
                     RIEGIKRVRFTSPHPKDFPLHLVEVMAEEPGICKHIHLPVQAGSDRILKLMRRTYTRS
                     DFLTLVEKIRALIPGIGLTTDIIVGFPGETPGEFDETFSLLQEVEFDAAYIFQYSERP
                     GTLAARKYADDVSGEEKSRRSVLLNALQNGISLKKKRARIGRIERVLVEQAGTRKSRA
                     ESQGHTDDNHLVILQEGNFTVGEMVDVRIHDATAHVLKGRPPL"
     gene            complement(1743..3035)
                     /gene="mtaB"
                     /locus_tag="PKM23_02345"
     CDS             complement(1743..3035)
                     /gene="mtaB"
                     /locus_tag="PKM23_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNY90342.1"
                     /translation="MEQPRKISFYTLGCRLNQAETAALQNRFSEAGYEIVDFREPADV
                     VVINTCTVTENGDNDTRRLVQRIHRAQARARIALIGCQSQMQGEALCRLPGVSWVAGN
                     AVKMELPEIIRRHSEGPPLLIAPPPGRGRFTMPAPAIDRSHTRANLKIQDGCDFFCSF
                     CEIPYARGRSRSREFDDLMGEARQLIAAGHREIVLTGVNIGTWSGEGRQLADLLGALT
                     GLAGLERLRISSIEMTTVPEEIFQLMQSTSLCRFLHIPVQSGSDPILKAMHRRHSAAQ
                     FAELIRRAAAEVPEICLGTDILTGFPGETEALFAETCDLLEALPLAYFHVFSYSERSH
                     ARSRLLPGKVSEEVKAERSARLRRLSLEKRHAWFRRFLGTHQLVLFEEEKKGVWSGVT
                     DTFIRVRVRSAGNLHNQLRPVRLEKIEELSVFGTLITA"
     gene            complement(3048..3533)
                     /locus_tag="PKM23_02350"
     CDS             complement(3048..3533)
                     /locus_tag="PKM23_02350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNY90343.1"
                     /translation="MAAKQYDQAPALAIDTQKSYLARLETSRGIIELELYPQYAPKTV
                     NNFVFLSREGFYDGVTFHRVIADFMIQGGDPTGTGRGGPGYRFEDEFRGNPLTHETGV
                     ISMANAGPGTNGSQFFITHSPQPHLNGRHTVFGKVVKGQEVVDGVRQGDVITRVTIEE
                     K"
     gene            complement(3648..4460)
                     /locus_tag="PKM23_02355"
     CDS             complement(3648..4460)
                     /locus_tag="PKM23_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNY90344.1"
                     /translation="MKTMQGDGGEVITITSIDDPRIADYRLLRDRALMQERGLFIVEG
                     ENPVLRLLASPCRTASLFVSEKRLGRIAPFVPAGTPLYVAPEELLARAPGFTFNRGVI
                     ACGIRQPLPAPEQLVVQLRPPALVVVCAGIGDAENLGQIVRTSAALGAGALLIGEGTI
                     DPFYRRVIRVSMGALFTLPIAEGGDLRAALPLLRDAGFRLYATHLSPDSTPLEESRPA
                     PLSAILLGNEGSGLPPQLTALCDERITIPMTRGADSLNVAAAAAIVLHHFRR"
     gene            complement(4484..5305)
                     /locus_tag="PKM23_02360"
     CDS             complement(4484..5305)
                     /locus_tag="PKM23_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90345.1"
                     /translation="MKRVLHWLVAIFILLGVVWLLRSWLMSRVFYQPDWYRDQQAADA
                     DWRNPPQPSAAREESPATAVQPEAGTENEPAGGLPEFPPLPQPQAPVAEPDRTNREGV
                     SAAEAPRTPAGRALTPLQRALEQKGTMRISADEFVPMLLAEMAGQDDFDPYDVVRGAK
                     TTIHRDRMIIEIMVNLEKFPADQLTPEGKRIFDQISGLLPAKALQQVYFKGDLRPVAS
                     GKRIVLTEASTISVGRFNFSLADLRKRFQIDPAIDMSHFAFSRFELQEGSIVLQR"
     gene            complement(5322..6896)
                     /locus_tag="PKM23_02365"
     CDS             complement(5322..6896)
                     /locus_tag="PKM23_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNY90346.1"
                     /translation="MLFAGLDSSTQGCKLIVLDLAAQKVMHLDAVHYDTDLDYDTRNG
                     VIQGLEAGASESDPRMWIEAIELLFGRMQDAGVPVAEIRAISVSGQQHGLVTLDAEGR
                     LSRPHSKLWNDFSTEEECRLLTEAVGGPEVMIAEVGNSQRTGYTAAKIFHLVRHEPEL
                     WRRTATCFLVHNYVNWWLTGGQEGGIRVMEPGDLSGMALWHPGTSRWSDRVLGAIAPD
                     LAAKLPPVRPSDQSIGTIGRALAERFGFHPECRIDAGCGDNMYGAVGTGNVAPGLVTI
                     SLGTSGTAYTVLEEPFIDPSGEIAAFRDSTGRHFPLLCVSNLANGYNAIIAEHGLSHA
                     RFNELMAKTPAGNEGRILIPWYMGERTPDVPLAAPLYFGFGLADFTVEKLGRAVLEGH
                     ILNLYDGYRKMPIEAREIRLTGGLSQSPAWCQAIADIFETEVVPVEGEGAAMGAALHA
                     AWVYEKENGREVALADLVQPFIQLREAMRKKPDPAHLDLYRPMKQAFFALSARVRGMA
                     GSEDPFLWRQRMQIQS"
     gene            complement(6910..7083)
                     /locus_tag="PKM23_02370"
     CDS             complement(6910..7083)
                     /locus_tag="PKM23_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90347.1"
                     /translation="MAEELLSAGKLAKEWGVTDAAVKKAIKELGIEPDAKKGACAMYG
                     KATAAKIKAALKK"
     gene            7297..8481
                     /locus_tag="PKM23_02375"
     CDS             7297..8481
                     /locus_tag="PKM23_02375"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNY90348.1"
                     /translation="MKIFVLNCGSSSLKFQLIEADTDELLAKGVVERIGASDAILTYK
                     PVGKEPVAQTREIPSHEAALSLVLGLLSDPRHGVISSMNEISGVGHRLVHGGEEFVSS
                     VRIDDKVKEGVHRCIQFAPLHNPHNLKGVEVCEQLMPGVPQVGVFDTAFHHTLPAEAY
                     TYALPMELYRKHRIRRYGFHGTSHAYVAQRAAEYIGRPIGELKIITCHLGNGASVTAV
                     DGGHSVETSMGFTPLEGLVMGTRSGDVDPALIPYIAKIEDLSMAQVDALLNKKSGMLG
                     LTEKTNDFREIEEAAEQGDEASILALQIFCHRLRKYIGAYMAVLGGLDALVFTAGIGE
                     HSPLVRAWSCRNLEAFGIALAPEKNQDNAFEISTGRVKVLVVPTNEELAIARETERIL
                     KG"
     gene            complement(8536..8610)
                     /locus_tag="PKM23_02380"
     tRNA            complement(8536..8610)
                     /locus_tag="PKM23_02380"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8576..8578),aa:Gly,seq:ccc)
     gene            8810..10837
                     /locus_tag="PKM23_02385"
     CDS             8810..10837
                     /locus_tag="PKM23_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNY90349.1"
                     /translation="MKEGAPPHGRGRAALFRIKSLDHIIAESERGEFRLNRVLGPVQL
                     TLFGIGAIIGAGIFATIGTAAAGDAFRPGAGPALMLSFIITAVVCGFTALCYAEFASL
                     VPIAGSAYTYSYATLGELIAWVIGWDLIIEYAVGNIAVAISWANYFKTFLRGFDIIIP
                     DWISMDYRTAARIVDAAGVQTVFRDAPHLFGLPIVFNLLAVAIVALISLVLIWGIRES
                     ARFNAVMVGIKIVVLTFFIVVGCCWIKPENWTPFAPNGWSGISAGAAIVFFAYIGFDA
                     VSTAAEETRNPSRDLPIGIIASLLICTIFYVVVAAVFTGLISYPELKATLATEQAEPL
                     TLALAHASPTLGWAVGIVAFGSVIAHTAVLFIFQLGQPRIFFSMSRDGLLPPLFRRVH
                     PRFRTPHVATLLTGVFVASFAAVASIDEMVDLTNIGTLFAFILVSAGIVVMRRRDPHR
                     PRPFRVPGGWPWALGLYLLLFVVVLLMPLAPGLKAGLLLGAALLFYAARNYIFPILGI
                     ISCLYLIYYLPPTSWLRFAAWLNIGFVIYAGYGVLHSRLEGEEGRSGPQALAYAARVG
                     AWLLVIGDALLFLTRGFDLVIHAGKELAGEGTLQRAAAIMAAVGTPERWLEASWFLII
                     PLLLNSLVLGPMVIRRALQAEKGGEDTRPALAAAALLSLAGLLYLGIVAMR"
     gene            complement(10850..12685)
                     /locus_tag="PKM23_02390"
     CDS             complement(10850..12685)
                     /locus_tag="PKM23_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNY90350.1"
                     /translation="MLLKPRTIELLAPARDPEAGRAAIDCGADALYIGAPRFGAREQA
                     GNSLQEIESLILYAHRFWARVYITLNTLLHDDELEEAVALVHRLHDLGADGLIIQDAG
                     LLEASLPPIPLIASTQMHNHTPERVAFLEGAGFQRAILARELSLEQIGAIRRSTQIEL
                     EGFVHGALCVSYSGQCYLSLARGGRSGNRGQCAQPCRLPYRLVDAAGKTISGRRHLLS
                     LRDLNLSEQLGELLDAGITSFKIEGRLKDASYVKNIVTWYRRALDPLLKERGLHAASS
                     GRALAAFTPDPAKSFNRGFTTYFIHGRGSDLTAWATPKHSGEAVGLVRRCRAGELQLE
                     GGVHPLHNGDGLTWFDAEGELQGGRVNRVEGESLLLDRAVDLPPGTRIMRNHDHLFLE
                     ALKKSSCSRLIAVVIELVEAEGGLVLKARDEEGVAVELSAEAIFEPARDPGQALQAAR
                     SQLAKSGGTLFAVQEVRVEWREPWFLPLSVLNRMRRDLLAALLEARLRAHPLCRTAIV
                     PNDLPFPARSLDFHGNVLNRLARAFYARHGVAEIAPAAESGIDLRGQRVMTTRYCLRG
                     ELGLCGGQLHAHGFTEPLHLIDDQNQRLRLDFRCSECEMDLYLDG"
     gene            complement(12808..13389)
                     /locus_tag="PKM23_02395"
     CDS             complement(12808..13389)
                     /locus_tag="PKM23_02395"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNY90351.1"
                     /translation="MNAIMQMIVQQLSGNAINKIARQLGIDSATASKAVGVAVPILIS
                     ALTRNSSTETGASSLHKALIKDHDGSIFDNLGSLIGDASGFSGSGILKHILGADQSTV
                     TSRVSQTAGVDSGTMSSIMEMVAPMVMGALGKTTREEGLDVSGLTELLTGQQQEAEAE
                     QPNIFGSLNKMLDSDGDGSSMDEIGGFLGKLFS"
     gene            14381..15163
                     /locus_tag="PKM23_02400"
     CDS             14381..15163
                     /locus_tag="PKM23_02400"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNY90352.1"
                     /translation="MLHSSLPVHLCKGCFGRCANALLPFLLILLPLTTAGAQTSGQEN
                     KGFLIQSQPGGATVLIEDEIIGKTPCTFPYQLAGKYRLFAEKRGYESVSREIDFSTRK
                     IETITFMLSPKTRGKAVLRSFFVTGWGQNYSEQPLKGRILMGLQAACLLSLGYTHQRC
                     LHFEDVYRVQLDSYRISSLKYDREAAAWQRLHSAHQDWKQADQWRQAMLYTAIGLHAL
                     NFLDALFIFPKNLRQIEFLAAPGVSQPLPAGSGFTISYTVPL"
     gene            15175..17016
                     /locus_tag="PKM23_02405"
     CDS             15175..17016
                     /locus_tag="PKM23_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNY90353.1"
                     /translation="MKTRSILAVWMFMIAVVACEKLSEPLRNTSQDYPGAPTHLVAQV
                     GDRSVHLSWNAPSGEIKLYYLFRQDSIQTGLRLIDSCTTTFYVEKGLINGQRYIYHVA
                     ACNTAGFTGEISDGVSARPGLYSIIINNGALYTNSSTITLRLIAPSGTRYVKIAADSS
                     FTHATWQLYSDTISWSLSPGDGKRSVFAVFLDQEGNQTGSPVVDEIYLDAKAAIYSLV
                     IPNAVAAYRTGDIVSFQMRTDEPYGNAVVAFGSVLGSIRLYDDATHGDKSANDGIYSQ
                     DYQIPPCEDVFNISITGSFTDAAGNISDEYISAQTLTIENPPLPVQLFEPERIGGKDK
                     AFHLTWTVNKDADFSLYKLFRSDVEKVDSTSLMVAISSIQTSNYCVDSLLAYNHKYYY
                     RVYVYDQRGLISGSNIVSGQTPADAAPQRAQLQVPLNVTTTSILLSWNQNTESDFAHY
                     RLFRSTQASVSSSDIQVTLISTRATTQFQDSGLLPNTNYWYVLYTTDLAGNMSISNII
                     SATTLPEEKPDAVLLAVPAASALKTLRLSWSVSQSSAFASYRVFRSTNASVDNSATPI
                     VIINNAATTTHDDTGLGSDMTYYYRIYVYDWMGRSTGSNTVYGTTLP"
     gene            17043..18005
                     /locus_tag="PKM23_02410"
     CDS             17043..18005
                     /locus_tag="PKM23_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNY90354.1"
                     /translation="MKHKKYGCIISLGLFLMLAYDAGAQEYMIGKDDVLEISFWQDKD
                     LNVTTRVDANGQISLPIGGIIKADSLTIEQLADKIVERISLYNRRITNVSIKVTEYGS
                     RKVYVMGHVKDPKKYTFEIIPNLWDIISEAGGPLETADLSNVLIIRKSADGDPRTMSV
                     DVSEVLRNREFQRLPKIEPGDNIYVSAIVGAAASTGIQAMQTQPNVLFIYGEVGNSGV
                     FTFNKELNLLEALITAGGPTSTAKLSEVRVIRKNGAYSSVTKVNVKKYADESSPSFFL
                     VKGGDTIYVPRKSVFRESVFWDIIMIFSGAALTSLAYSLVTAED"
     gene            18030..19595
                     /locus_tag="PKM23_02415"
     CDS             18030..19595
                     /locus_tag="PKM23_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNY90355.1"
                     /translation="MSTRKGIDWQMWWQLLLRRRYYIIISMFIALAGGLYLALTTVPV
                     YQSASTVMIVDANLLSGASLRFVPSTSQVNEIDYFRRRITSEGFLLRLLDSLEVEQDP
                     KVVLKIDQLCAQNPFIEREVIAKQVYIEHLFKRISTRMKAYNLIEISAEGPSSEEAFR
                     LATLVTELAIAESQQSQIQSVSAVSSFSSQQLDIYRKRLEDTENRLNAFKTSLTVQGL
                     SENELSREKLSEIQSVKLSTDIDLQAKREQMQKLLSGSLRDITLSYRSDLDQTTRDLR
                     QRVLKRTRDVCELLKKFSWRDVEIILLNEEIGQLKEQIYESIRNRVTVDLGTKSSTVV
                     DAAIKLEELRLDVDILQNSTEILQEIIASHNDLIRRQPSQEGLRAKLEREVQINREIY
                     EALLQANQGSQMRESAEVTETKMRFKLLSPPQRPLERIKPQRARMMFIALFLGLGLGL
                     GMVMAREYMDTSLTSIDDVTNYFKIPVLAAIPKIETRLELTRQKHRRYAAAVAIPLIA
                     LVSVMLIYRIFIR"
     gene            19607..20314
                     /locus_tag="PKM23_02420"
     CDS             19607..20314
                     /locus_tag="PKM23_02420"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNY90356.1"
                     /translation="MKQNGTLKGISEYSEHSPYFTEFYRLYARMRAPIEGRIPQIFMV
                     TSAIASEGKTTISAYIALTAALSTKSNHLVMDTDLNRPTLHKKLGLEQVNGLTEILSD
                     GLELQQAIQTTPYSGLHVISAGRNVARPFELLSFGRVENLFNQLRLFYDTIILDAPPI
                     LHVGDTLKLAEYVDGILLVVLSGRTNREIVKRAIELLDETGKPILGIVMNDMGEVLPY
                     YYQHKYYSYHYGSSEVK"
     gene            20330..21547
                     /locus_tag="PKM23_02425"
     CDS             20330..21547
                     /locus_tag="PKM23_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HNY90357.1"
                     /translation="MASAKLARSIAFNVPCIGAEEIGSVVETLRSGWLTTGSKAAQLE
                     KEVAAFTRARYAVAVNSCTAGLHLSLLALGVGPGDEVITTPYTFSATGETIVHTGARP
                     VLVDVERGGFNIDPQKIAAAITARTKAIVPVHIAGDPCNMTEILSLARRKGIHVVEDA
                     AHALGTWYDGNPVGSISTLTVFSFYATKNLTTGEGGMVTTDSEKLAKNIRQLSLHGLS
                     HDAWNRYSDRGSWRYEICTLGYKCNLSDIQAAIGIAQMHKFETMQARRRKLVEAYQEG
                     LAGLPDLILPEEREGTRHAWHLYIIRLAEERAREQRDDLIEQLKGMGIGVSVHFIPLH
                     LHRYYQQTFNYRPGDFPEAEKQYQAAISLPLYPTLRREEVAFICRALRRLLHRPAPHR
                     SRRGISHHDKAIV"
BASE COUNT         4304 a         6228 c         6511 g         4565 t
ORIGIN      
        1 gcacgctggg agacgaaggc ggcaaagaag agggcgtcaa ggttgccgtt ctccatacgc
       61 gtcaggtcga tcttgccacg gtcgcgcgtt cccgacgggt ggacggcccc gatatcccag
      121 gtcgtatcca gcatatgcag gggagtgtcg cagtgagtgt cgatggtcag cagctcgcga
      181 tgcagcttga gcgcggccga atcgggatcg gcaccgcgca ggggcaggac cagggcgagc
      241 agaagaccgg cggcgtggat cagtttcagg tttttcaaac tcttcctcgt gtcaaagagg
      301 tgcgatgtgg cggaggcgcg gtccggaaag cgggcccgct ttgttctctt cctccggcta
      361 gataaatata cggcagccgc gcagggatgc ggaggagttt ttagagcggt ggccggccct
      421 tgagcacatg ggcggtggcg tcgtggatgc gcacatccac catttcgccc accgtaaagt
      481 tcccctcctg caggatcacc aggtggttgt cgtcggtatg cccctggctc tctgcgcggg
      541 acttgcgcgt cccggcctgc tcgaccagca ccctctcgat gcggccgatg cgggcgcgct
      601 tttttttcag cgaaatcccg ttctgcaggg cgttgagcag gactgagcgc cggctcttct
      661 cctcgccgga aacgtcgtcg gcatattttc tcgccgccag ggtgccgggc cgctctgaat
      721 actgaaagat gtaggcggca tcgaactcga cctcctgcaa gagggagaag gtttcatcga
      781 actcgccggg ggtttcgcct ggaaaaccga cgatgatgtc ggtggtgagg ccgatcccgg
      841 ggatgagggc gcggatcttc tccaccaggg tgagaaagtc gcttcgggta taggtgcgcc
      901 gcatcagctt gaggatgcgg tcgctgcccg cctggacggg gagatggatg tgcttgcaga
      961 taccgggctc ttcggccatc acctccacga ggtggagggg aaaatccttg ggatggggcg
     1021 aggtgaaacg cacccgcttg atgccctcga tccggctcac ctcccggagc aaggcggcaa
     1081 agtcgtgctg ctcataacga tacgaattga cattctgtcc cagcagggtc acctggcaaa
     1141 agccatcggc agcgaggcgg cgcacctcat cggtgatgct ccggggcgaa cggctgcgtt
     1201 cgcggccgcg ggcaaagggg acgatgcaga aagagcaaaa gttgttgcag ccgcgcatca
     1261 ctgcgatcca ggcgttgaca ccgccccggc ggagggggta gatgtcagca taggtctcaa
     1321 agtcggagag ctcggcggcg tagagctctt ttttctccgc tgcagcggtt gtgatcagcc
     1381 cgggcaggcg gcggtagctg tccggaccgg cgaaaaagtc gaccggcagc cggcggtttc
     1441 cgcccagctg cttcttcagt cctgtggcca tgcagccgac cacgccgagg cgaagcctgg
     1501 cgccgttggg caggcggcgg atctcatgga tgcggttgac cacccgcaag gcggcgtgtt
     1561 cccgcacggc gcaggtgttg atcagcacca catccgcctg ctccggggca tcggtgctct
     1621 ccagatcggc ctcgtccaga atggcgcgga tgatctcgct gtcatattcg ttcatctgac
     1681 agccataggt ttcgatatat actttcacgc cgggtgcttc actttcgatc catagatttt
     1741 tatcacgctg tgatgagggt cccaaatacc gagagttcct cgattttttc gaggcggacg
     1801 gggcgcagct gattgtgcag gttgccggcg ctgcgtacgc gcacccggat aaaggtgtcg
     1861 gtcacgccgc tccagacgcc ctttttctcc tcctcgaaga ggacgagctg gtgggtgccg
     1921 aggaagcggc ggaaccaggc atggcgtttt tcaaggctga gccggcgcag ccgggcgctg
     1981 cgctctgcct tgacctcctc gctcaccttg ccggggagga gtcggctgcg ggcgtggctg
     2041 cgctccgaat agctgaagac gtggaaatag gcaaggggaa gggcttcgag caggtcgcag
     2101 gtctccgcaa aaagagcttc ggtctcgccg ggaaaaccgg tgaggatatc tgtgcccagg
     2161 cagatttccg gcacctctgc ggcggcgcgg cggatcagct ccgcgaactg ggctgcgctg
     2221 tggcggcggt gcatcgcctt gaggatgggg tcgctgccgc tctggacggg gatatggaga
     2281 aagcggcaga ggctggtgct ctgcatgagc tggaagatct cttccggtac ggtggtcatc
     2341 tcaatcgagg agatgcgcag gcgttccagc cccgccaacc cggtcagggc gccgagcaga
     2401 tcggccagct gccggccctc cccgctccag gtgccgatat tgactccggt gaggacgatt
     2461 tcacggtggc cggcggcgat aagctgccgg gcttcgccca taaggtcatc gaactcgcgg
     2521 ctgcggctgc ggccgcgggc ataagggatc tcgcagaaag agcagaaaaa atcgcagccg
     2581 tcctggatct tgagattggc gcgggtgtgg ctgcgatcga tggccggggc cggcatggta
     2641 aagcgtccgc ggccgggggg aggggcgatc agcaagggag gcccctcact gtgcctgcgg
     2701 ataatctccg gcagctccat cttgacggcg ttgccggcca cccaactgac cccgggcagg
     2761 cggcagaggg cctcgccctg catctgcgac tggcagccga tgagggcgat gcgtgcgcgg
     2821 gcctgggcgc gatggatgcg ctgcacgagc cgccgcgtat cgttgtcgcc gttctccgtc
     2881 accgtgcagg tgttgatcac caccacatcc gcaggctccc tgaaatcgac gatctcatag
     2941 cctgcctcgc tgaagcggtt ctgcagggcc gcggtctcgg cctggttcag ccggcagccc
     3001 agggtgtaaa aggatatttt gcggggttgt tccacgcggc tgccggccta cttttcctcg
     3061 atggttaccc gggtgataac gtcgccctgg cgcacaccat ccaccacctc ctgccccttg
     3121 accaccttgc cgaagacggt atggcgcccg ttgagatggg gctgcgggga gtgggtgata
     3181 aagaattggc tgccgttggt gcccggtccg gcgttggcca tggagattac cccggtctca
     3241 tgggtgaggg gattgccgcg aaattcatcc tcgaagcggt agccgggacc accgcggccg
     3301 gttccggtcg gatcgccgcc ctggatcatg aaatcggcga tgacacggtg aaaggtgacg
     3361 ccgtcgtaaa aaccctcacg gctgaggaag acgaaattgt tgaccgtctt cggggcatac
     3421 tgggggtaga gctccagttc gatgatgccg cggctggtct cgagccgggc gaggtagctc
     3481 ttttgggtgt cgatggccag ggcgggcgcc tggtcgtatt gtttggcagc cactttcttc
     3541 tcctgtaaat gaggtggttt acgtcccaaa agataccgat tccggacgag aaaaccaagc
     3601 aatttggccg cgaggccgcc gggggctgcc tcagtgcgct gcagggctca cctgcggaag
     3661 tgatggagga cgatagcggc tgcggcggcc acattgaggg aatcggcgcc ccgcgtcatg
     3721 ggaatggtga tgcgctcatc acagagcgcg gtgagttggg ggggcaggcc gctgccctcg
     3781 ttgccgagga ggatcgcgct caggggggca ggccggctct cctccagagg ggtcgagtcg
     3841 ggggagaggt gggtggcgta gaggcgaaaa cccgcatccc gcagcagggg cagggctgcg
     3901 cgcaagtctc cgccctcggc gatggggagg gtgaacaagg cccccatcga gacgcggatg
     3961 acccgccggt aaaagggatc gatggtcccc tctccgatga gcagagcccc ggcgccgagg
     4021 gcggcggagg tgcgtacgat ctggccgagg ttttcagcgt cgccaatccc cgcacagacc
     4081 acgacaaggg cagggggccg cagctgcacc accagctgct ccggcgcagg caggggctga
     4141 cggatcccgc aggcgatgac gccgcggtta aaggtgaaac cgggggcccg ggcaaggagc
     4201 tcttcagggg cgacatagag aggggtgccg gccgggacaa agggggcgat gcggccgagc
     4261 cgtttttcgc tgacgaagag ggaggcagtg cggcaagggc tggcgagcag gcggagcacc
     4321 ggattctctc cctcgacgat gaagaggccc cgctcctgca tcagggcgcg gtcgcggagg
     4381 aggcggtaat cggcgatgcg cggatcatcg atcgaggtga tagtgatgac ctctccgcca
     4441 tcgccctgca tggtcttcat tttttcctcc ccgcagctgc cggctaccgc tgcagcacga
     4501 tggagccctc ctgcagctcg aagcggctga aggcgaaatg gctcatgtcg atggcgggat
     4561 cgatctgaaa acgctttctg agatcggcga gactgaaatt gaaacgcccc acgctgatcg
     4621 tcgaggcctc cgtgagcaca atgcgcttgc ccgacgccac cggccggaga tcgcccttga
     4681 aatagacctg ctgcaaggcc ttcgcgggca gcagtccgct gatctggtcg aaaatgcgct
     4741 tcccttccgg ggtgagctga tcggcgggga atttttcgag attgaccatt atctcgatga
     4801 tcatacggtc gcgatggatg gtggtcttgg ccccgcgcac aacatcatag ggatcgaaat
     4861 cgtcctggcc ggccatttcc gccagcagca tggggacgaa ttcgtcggcg ctgatgcgca
     4921 tcgtgccctt ctgctcgagg gcgcgctgca ggggagtgag tgcgcggccg gcgggagtgc
     4981 gcggcgcctc cgctgcggag acgccctcac ggttggtgcg gtcaggctca gcgaccggag
     5041 cctgcggctg cggtagcggc gggaattcgg gcagaccgcc ggccggctcg ttttctgtgc
     5101 cggcctccgg ctgcacagcg gtcgcaggac tctcctcccg ggctgcggaa ggttgcggcg
     5161 gatttcgcca atctgcatcc gccgcctgct ggtcgcggta ccagtcaggc tgataaaaga
     5221 cccggctcat gagccaggag cgcagcagcc agaccacgcc cagcaggatg aagatggcca
     5281 ccagccagtg cagtactctt ttcatgtcgt gcccgcctcc ttcaggattg gatttgcatg
     5341 cgctggcgcc agaggaaggg atcctcggac ccagccattc cgcgcacccg ggcgctgagg
     5401 gcgaaaaagg cctgcttcat cggccggtag aggtcgagat gagcgggatc cggcttttta
     5461 cgcatggcct cgcgcagttg gatgaagggc tgcaccaggt cagccagggc aacctcccgg
     5521 ccgttctcct tttcgtagac ccaggcggcg tgcagggccg cccccatcgc cgccccttca
     5581 ccctcgaccg gcaccacttc ggtctcgaag atgtccgcga tggcctggca ccaggccggc
     5641 gactgggaga gcccgccggt gaggcggatc tcgcgcgcct cgatcggcat cttgcggtag
     5701 ccgtcataga ggttgaggat atggccctcg aggacggccc ggccgagctt ttccacggtg
     5761 aaatcggcca gcccgaaacc gaaatagagg ggggccgcca ggggcacatc cggggtgcgc
     5821 tcgcccatat accagggaat cagtatcctg ccctcattgc ccgctggagt tttcgccatc
     5881 agctcattga atcgggcatg gctgagcccg tgctcggcga tgatggcgtt gtagccgttg
     5941 gccagattgg agacgcagag aagggggaaa tggcgcccgg tgctgtcgcg aaaggcggcg
     6001 atttcgccgc tggggtcgat gaaaggctcc tcgaggacag tataggccgt gccgctggtg
     6061 ccgaggctga tggtcaccag ccccggcgcc acgttgccgg tgccaacggc cccgtacatg
     6121 ttgtcgccgc agccggcgtc gatccggcac tccggatgga aaccgaatcg ctccgccagg
     6181 gcgcggccga tcgtgcctat gctctggtcc gagggcctga ccggggggag cttggcggcg
     6241 agatcaggtg cgatggcgcc gagcacccgg tcggaccagc gactggtgcc ggggtgccag
     6301 agggccatgc cggagagatc gcccggctcc atgacgcgaa tgccgccctc ctgaccgccg
     6361 gtcagccacc agttgacata gttgtggacg agaaagcagg tggccgtcct tcgccagagt
     6421 tcgggctcgt ggcggaccag atgaaagatc ttggcggcgg tgtagccggt gcgctggctg
     6481 ttgccgacct cggcgatcat tacctcgggg ccgccgaccg cttcggtgag cagccggcac
     6541 tcctcctcgg tgctaaagtc gttccagagc ttgctgtgcg gcctcgacag ccgtccctcc
     6601 gcatccaggg tcaccagtcc gtgctgctgg cccgagaccg agatggcgcg gatttcggcc
     6661 accggcacac cggcgtcctg catgcggccg aagagcagtt cgattgcctc gatccacatg
     6721 cggggatcgg actcagaggc ccccgcttcc agcccctgga tcaccccgtt gcgcgtatca
     6781 taatccaggt cggtgtcgta atggaccgcg tcaaggtgca tgaccttctg cgcagcaagg
     6841 tcgaggacaa tcagcttgca tccctgggtg gaactgtcga gaccggcaaa aagcatgggg
     6901 acctcctgtt tattttttga gggccgcttt gattttggca gcggttgcct tgccgtacat
     6961 ggcgcaggcg ccttttttgg catcgggctc gatgcccagt tccttgatcg cctttttcac
     7021 ggcggcatcg gtgacgcccc actccttggc gagcttgcca gcggaaagca gttcttcagc
     7081 cattgcatcc tcctattggt attccttgtt cggtccctcc cgggtctgcg ggggcagggg
     7141 cgttaataac ataatggttt tccggcaaag tttcaagcta atcctgcagc actgtacaaa
     7201 aataaaacca tttggtttta gagttcaatt ttataaatta tacatcttga aagataatcc
     7261 taatcaacag aacaagcaac gcgtcgaggg agttccatga agatatttgt actcaactgc
     7321 ggcagttctt cgcttaaatt tcagctcatt gaagcggaca ctgacgagct gctcgctaaa
     7381 ggggtggtcg aacgcatcgg cgccagcgat gccattttga cctataaacc ggtggggaaa
     7441 gagccggtcg cgcagacgcg cgaaatcccc agccatgagg ccgccctgag tctggtgctc
     7501 ggactcttga gcgacccccg gcacggggtc atctccagca tgaacgagat cagcggcgtc
     7561 ggtcaccgcc tcgtgcacgg tggcgaggag ttcgtcagct cggtacggat cgatgacaag
     7621 gtcaaggagg gggtgcaccg ctgcatccag ttcgcccccc tgcacaatcc ccacaatctc
     7681 aagggcgtcg aggtctgcga acagctcatg cctggcgtcc cccaggtggg ggtcttcgat
     7741 accgcctttc atcacaccct gcccgccgag gcctatacct acgccctgcc gatggagctc
     7801 taccgcaagc accgcatccg ccgctacggc tttcatggca cctcccacgc ctatgtcgcc
     7861 cagagggcgg ccgagtacat cggccggccg atcggggagc tgaagatcat cacctgccat
     7921 ctcggcaatg gcgccagcgt gaccgcggtc gacgggggcc attcggtgga aaccagcatg
     7981 ggcttcaccc cgcttgaggg gctggtcatg ggcacccgtt cgggtgatgt cgatcccgcc
     8041 ctcatcccct atatcgccaa gatcgaggac ctctcgatgg cccaggtgga tgccctgctc
     8101 aacaagaaga gcggcatgct tggcttgacc gagaagacca atgacttccg cgaaatcgag
     8161 gaggctgccg agcagggcga cgaggcctct atcctcgccc tgcagatctt ttgccaccgc
     8221 ctgcgcaaat acatcggcgc ctacatggcg gtcctgggcg ggcttgatgc cctggtcttc
     8281 accgccggca tcggggaaca ctctcccctc gtgcgggcgt ggagctgccg gaatctcgag
     8341 gccttcggca tcgccctcgc tcctgaaaag aatcaggaca acgctttcga gatcagtacc
     8401 ggccgggtca aggtcctggt ggtcccgacc aacgaggaac tggccatcgc ccgcgagacc
     8461 gagcgcattc tcaagggctg aagggcacca aaaacagaaa agccttgcat ccgaagggat
     8521 gcaaggcttt ttttgtggag cgggagacgg ggatcgaacc cgcgatatca agcttgggaa
     8581 gcttgcgttc taccactgaa ctactcccgc gctcttatat gataataaat tttgcgggga
     8641 aatgcaagcc cttttctcgc acctgagagc cactggccct gcccctgctc actcacccgg
     8701 aggaaaaaat ttccgcaaaa gggtttgcat ttgcccctca atttgatgta tattcatgca
     8761 atccgctgac cggcagcacc gcagataccc atccgcagga gcgcagagca tgaaagaggg
     8821 agcaccaccg cacggccggg gcagggccgc acttttcagg atcaaatcgc tcgatcacat
     8881 catcgccgaa tcggagcgcg gcgaattccg cctgaaccgg gtgctcggcc cggtgcagct
     8941 cacccttttc ggcatcggag cgatcatcgg cgcgggtatt ttcgccacca tcggcaccgc
     9001 cgccgccggc gacgccttcc gcccgggcgc cggcccggcc ctgatgctct ccttcatcat
     9061 caccgccgtg gtctgcggct tcaccgccct ctgctacgcc gaattcgcct ccctcgtccc
     9121 catcgccggt tcggcctaca cctactccta cgccaccctg ggcgagctca tcgcctgggt
     9181 gatcggttgg gacctgatca tcgagtacgc cgtcggcaac atcgccgtgg ccatctcctg
     9241 ggcgaattat ttcaagacct ttctgcgcgg attcgacatc atcatacccg actggatctc
     9301 gatggattac cgcactgcgg cgcggattgt agacgcggcc ggggtgcaga ctgtctttcg
     9361 cgacgccccc cacctcttcg gactgccgat cgtcttcaat ctcctcgctg tcgccatcgt
     9421 cgccctcatc agcctggtgc tcatctgggg catccgcgag agcgcccgct tcaacgctgt
     9481 catggtcggc atcaagattg tcgtgctgac cttttttatt gtcgtcggct gctgctggat
     9541 caagccggaa aactggaccc cctttgcccc caacggctgg tcggggataa gcgccggggc
     9601 ggcgatcgtc ttctttgcct atatcggctt tgatgcggtc tcgaccgcgg cagaggagac
     9661 gcgcaatccc agccgcgacc tgcccatcgg catcatcgcc tcgctgctca tctgcaccat
     9721 tttttatgtt gtggtcgccg cggtctttac ggggctgatc tcctatccgg aactcaaggc
     9781 caccctggcc acggagcagg ctgagcccct caccctggcc ctcgcccatg ccagtccgac
     9841 cctgggctgg gcggtcggca tcgtcgcctt tggctcggtc atcgcccata ccgccgtgct
     9901 tttcatcttt cagctcggcc agccgcgtat ctttttttcc atgtcacgcg atggcctgct
     9961 gccccctctc ttccggcggg ttcacccccg ctttcgcacc ccccatgttg cgaccctgct
    10021 taccggggtt tttgtggctt ccttcgccgc cgtggccagc attgatgaaa tggtcgacct
    10081 gaccaacatc ggcactctct tcgccttcat cctggtcagc gccgggattg tggtgatgcg
    10141 gcggcgggac ccccaccggc cgcggccctt ccgcgttcct ggcggctggc cctgggccct
    10201 cggcctctat cttctgttgt tcgtggtggt gctgctgatg ccactcgcgc cgggcctcaa
    10261 ggcggggctc cttctcgggg ccgccctgct cttctatgcc gcccgaaact acatttttcc
    10321 catcctcggc ataatcagct gcctctatct gatctattat ttgccgccga cctcctggct
    10381 gcgcttcgcc gcctggctta atatcggctt tgtcatctac gccggttacg gggtgctgca
    10441 cagccggctg gagggcgagg aggggcgaag cggcccgcag gccctggcct atgccgcgcg
    10501 ggtgggggcc tggctgctgg tgatcggcga tgcgctgctt tttctgacgc ggggattcga
    10561 cctcgtcatt cacgccggca aggagctggc cggggagggg accctgcaac gggctgcggc
    10621 cataatggcg gcggttggca cgccggagcg ctggctcgag gcctcctggt tcctcattat
    10681 cccgctcctg ctcaactccc tggtgctggg gccgatggtg atccggcgcg ccctgcaggc
    10741 tgaaaagggg ggggaggaca cccggccggc cctggcggct gcagcgctcc tctcccttgc
    10801 tggtctgctc tatctgggta ttgtggcgat gagataggag tgctgcgggt cagccgtcga
    10861 gatagagatc catctcgcac tcgctgcagc ggaaatcgag gcgcagccgc tgattctggt
    10921 catcgatgag gtggagcggt tcggtgaagc cgtgggcgtg cagctgcccc ccgcacagcc
    10981 cgagttcacc gcgcaggcag tagcgggtcg tcatcacccg ctggccgcgc aggtcgatgc
    11041 ccgattcagc ggcgggggcg atttcggcca cgccatgccg ggcataaaaa gcgcgggcca
    11101 ggcggttgag cacattgccg tgaaaatcga ggctgcgcgc aggaaagggg agatcgttcg
    11161 ggactatggc cgtccggcag aggggatggg cgcgcagccg ggcctcaagg agcgcagcga
    11221 ggagatcgcg gcgcatgcgg ttgagcaccg agagggggag aaaccagggc tcgcgccatt
    11281 ccacccgtac ctcctgcact gcgaaaagag tacccccgct tttggccagc tgcgagcggg
    11341 ccgcctgcag ggcctgcccg ggatcccggg caggctcgaa aatggcctcc gcagagagct
    11401 ccaccgcgac tccctcctca tccctcgcct tcagcaccag tccgccctcc gcttcaacca
    11461 gttcgatcac cacggcaatc agccggctgc agctgctctt tttgagggct tcgaggaaca
    11521 ggtggtcatg attgcgcatg atgcgggttc cgggggggag atcgacggcc cggtcgagga
    11581 ggaggctttc cccctcgacg cggttgactc tgcccccctg cagctccccc tccgcgtcga
    11641 accaggtgag tccgtcgccg ttgtgcagag gatggacacc gccctcgagc tgcagttccc
    11701 cggcccggca gcgccggacc agtccgaccg cctcgccgct gtgcttgggg gtggcccagg
    11761 cggtgaggtc gctgccgcgg ccgtggatga aataggtggt gaagccgcgg ttgaagcttt
    11821 tggcgggatc gggggtgaag gcggcgaggg cacggccgga ggaagcggca tgcagcccgc
    11881 gctctttcag cagggggtca agtgctctgc gataccaggt gacgatgttc ttcacatagg
    11941 aggcatcctt gagccggccc tcgatcttga acgatgtgat gccggcatcg agcagctcgc
    12001 cgagctgttc cgagaggttg aggtcgcgca aggagaggag gtggcggcgg ccgctgatgg
    12061 ttttgccggc cgcgtcgacg aggcggtagg gcaggcggca gggctgggcg cactgcccgc
    12121 ggttgccgct gcggccgccg cgggcgaggc tgagatagca ctggccgctg taggagacgc
    12181 agagggctcc gtgcacgaag ccctccagct cgatttgggt cgagcggcgt atggccccga
    12241 tctgctcgag gctgagctcg cgcgccagga tagcccgctg aaatcctgcc ccttcgagaa
    12301 aggcgacccg ctccggggtg tgattgtgca tctgggtgct ggcaatgagg gggatagggg
    12361 ggagggaggc ctccagcagc cctgcatcct ggatgatgag gccgtccgcc ccgagatcat
    12421 ggagacgatg gacgagcgca acggcttcct ccagctcgtc gtcgtgcagg agggtgttga
    12481 gggtgatgta gaccctggcc cagaagcggt gggcataaag aatcagggat tcgatctcct
    12541 gcagggagtt gcccgcctgt tcgcgggccc cgaaacgggg cgcgccgata tagagggcat
    12601 cggcaccgca gtcgatggcg gcacggccgg cttccgggtc gcgtgccggg gcgagaagtt
    12661 cgatggtgcg gggtttcagc agcacagcat gcccttgtcc aataaacagc tcgtggagat
    12721 gcagcagata taaaaagcct gcatggaatc atgcaggctg gtctggcttg aggaaagtaa
    12781 ccttcccgtg cgggaagggg aatggaatca gctgaagagt ttacccagga agccgccaat
    12841 ctcgtccatc gagctgccat cgccgtcgct gtcgagcatc ttgttgaggg agccgaaaat
    12901 attgggctgc tcggcctccg cctcctgctg ctggccggtg agaagctcag ttaggccgga
    12961 gacatcgagt ccctcctccc gggtggtttt gcccagggcg cccatcacca tcggggccac
    13021 catttccatg attgagctca tggtgccgga atccacacct gcggtctggc tgacgcgtga
    13081 agtgaccgtg gactggtccg cgccgaggat gtgcttgaga atgccggagc cggagaaacc
    13141 ggaggcgtca ccgatcaggc tgccgaggtt gtcgaaaatg gagccgtcat gatccttgat
    13201 cagggccttg tgcagtgagg aggcgcctgt ttccgtggag gaattccggg tcagggcgga
    13261 gatgaggatc ggcacagcca ctccgaccgc tttggaggcc gtggcggagt cgattccgag
    13321 ctgtctggcg attttattga tggcattgcc gctcaactgc tggacgatca tttgcatgat
    13381 tgcattcatt aaaaggctcc ctatgctaga gaaatatggt tgtttgctat ttcaacaatc
    13441 gctgcagccg atttgttccc ctggaaaaaa acggggctat gacgacagtg aggataattc
    13501 tggcgagaac agggggcagg atcacgcgcc ctcgcccgga gagggaacaa aagaagccac
    13561 tggcgggaat tccttagcca taattggcgc tttagtggtc agatttgcac aatgaaccac
    13621 gaaatttgca caataatggc gtatagccgt taaaaagatg aaaaaaactt acttttctat
    13681 tgacatttag aatcacgctc tgtatatttc ttgtacagag tccagcaatg gttaaccctt
    13741 tgtaaaaaag ggcatgggga aaactcctag cattatcctt aacaaaagca aaaatgctcc
    13801 tgctacaaac cttaataaat tgatgcaatt ggtggtgagg ggtaggggtt tttgcggtat
    13861 caaggtgttc taggggaagc tttggaaatc cggactatca ttccttcttc tgcgtgactg
    13921 cttccggctt tgttgcgcac cttctatcag ccaaaatccc gcacgtttta ttcttcgtcc
    13981 atggaatgct tttggggagg cattgtcctc gcggttctct atcgatgagt ggcaactttg
    14041 ttcatttgca gcgtttaact tcgatccgac aatttcgctc agggattatt ccattctggc
    14101 catttcttcg gggggatcgt catgatggaa agcaacagtc tgtaatcatc tgaccagaca
    14161 gggcagagaa ttccttcctg ctcaagtaat tttattgtaa aaaaaaagct gccagcagac
    14221 cggccaagtc tgtagtgctg gcagcgtcac cacacacgag gtctatgcct gagcataggc
    14281 cgcggtgggc tttttttgtt ctgcacaaat tacaaaacag ggccaagttt gtcaagcgtt
    14341 atttttttga ataacgttac atgagaatgg cgagggatcc atgctgcatt ccagtcttcc
    14401 ggttcatctg tgcaaaggtt gttttgggcg ctgcgcaaac gccctgctgc cgttccttct
    14461 gatcctgctc cccctgacta cggcaggggc gcaaaccagc gggcaggaga acaagggctt
    14521 cctgatccag tctcagccgg gcggcgctac ggttttgatc gaggacgaga tcattggtaa
    14581 aaccccgtgt accttcccct accagcttgc gggcaaatac cgcctcttcg ctgaaaagcg
    14641 gggctatgag tcggtctccc gagagatcga tttcagcacg cgcaaaatcg agaccatcac
    14701 cttcatgctc tctcccaaaa cgcggggcaa ggccgttttg cgctcctttt tcgtcactgg
    14761 ttgggggcag aattacagcg aacagcctct gaagggcagg atcctcatgg gacttcaagc
    14821 ggcctgcctg ctctcactgg gatatacgca tcaacgctgc ctccatttcg aggacgtgta
    14881 cagggtgcaa ctggattctt accgaatctc gagtctcaag tacgatcggg aagccgcagc
    14941 ctggcagaga ctgcactccg ctcatcagga ctggaagcag gccgaccagt ggcggcaggc
    15001 catgctgtat accgcaatcg gactgcatgc cttgaatttt cttgatgccc tgtttatctt
    15061 tcccaaaaat ttacgacaga ttgaatttct tgctgcccca ggggtaagcc agcccctccc
    15121 ggctggcagc ggttttacga tttcgtatac agttccgcta taggaggatg gtccatgaag
    15181 acaagaagca tccttgcagt gtggatgttt atgattgccg tcgtggcttg tgaaaaattg
    15241 agtgagccat tgcgcaatac gagccaggac tatcccggtg cgccaaccca tctggtcgca
    15301 caggtgggtg accgttcggt ccatcttagc tggaatgccc cctccggtga gatcaagctt
    15361 tactatcttt tccgtcagga cagcattcag accggtctga ggctgattga ttcctgtacg
    15421 actacctttt acgttgagaa gggcctcatc aatggtcagc gttacatcta tcacgtcgca
    15481 gcttgtaaca cggcaggctt tacgggagag atatcggatg gtgtctccgc tcgtccaggc
    15541 ctctattcga taatcattaa caacggcgca ctctatacca attcatccac cattacactc
    15601 aggcttatcg ctcccagcgg cacgcgttat gtcaaaattg cagcggactc atcttttacc
    15661 catgcgacct ggcagctgta cagcgatacc atctcctggt cactatcccc tggagacgga
    15721 aaaagatctg tcttcgccgt gtttctcgat caggagggca atcagaccgg atcgccggtt
    15781 gttgatgaaa tctacctgga tgccaaagcg gcgatttaca gcctcgtaat ccccaacgcc
    15841 gtcgcggcct atcgtacggg tgatattgtc agctttcaga tgagaacgga tgaaccgtat
    15901 ggcaatgctg tggtcgcctt tggctctgtt cttggttcca tacgtctgta tgatgacgct
    15961 acacatggtg ataaaagtgc caacgatggc atttattctc aggattatca aatcccgccc
    16021 tgcgaggatg tttttaacat atccataacc ggctcgttta ccgatgctgc aggcaatatt
    16081 tcggatgagt atatctcagc acagaccttg acgatagaga atcctccact gcccgtgcag
    16141 ctgttcgagc ccgagcggat tgggggcaaa gacaaagcct tccatttgac atggaccgtc
    16201 aacaaggatg ccgatttctc tctttacaag cttttccggt cggatgtgga gaaggtcgat
    16261 tccacctctc tgatggtggc catctcctcc attcagacct ccaactactg tgtcgattca
    16321 ctgctcgcct acaatcacaa atactattat cgggtctatg tctacgatca gaggggattg
    16381 atctccggca gcaatatcgt cagcggtcag acacctgcag atgccgcacc gcagagggct
    16441 cagctgcagg tccccttgaa tgtgacgacg acctcgatat tgctgagctg gaatcagaat
    16501 acagaatcgg attttgcaca ttatcgtctg ttccgcagca cacaggcctc ggtgagcagc
    16561 agcgatatcc aggtcaccct gatttcgact cgcgcgacca ctcagttcca ggatagcgga
    16621 ctcctgccga atacaaatta ctggtatgta ctttacacaa ctgatcttgc cggcaatatg
    16681 agtatcagca atatcatttc ggcgaccacg ctccctgaag aaaaacccga cgctgtcctg
    16741 ctggcggtgc cggctgccag cgcattgaaa accttacgcc tctcctggag tgtaagccaa
    16801 tcgtccgctt ttgccagtta tcgtgttttt cgatcgacca acgcctccgt cgataattct
    16861 gcaactccaa ttgtcatcat aaacaatgcg gctaccacaa ctcatgatga tacggggttg
    16921 ggcagcgata tgacctatta ctacagaatt tatgtgtacg actggatggg ccggtccacc
    16981 ggaagcaaca ctgtttatgg gaccaccttg ccctgacgaa ggtgacaacc aggagagcaa
    17041 taatgaaaca caaaaagtac ggctgtatca tttcactggg cctctttctg atgctggcct
    17101 atgatgcagg ggcacaggaa tacatgattg gcaaggacga tgtgctggag atcagttttt
    17161 ggcaggataa ggatctgaat gtcaccaccc gggttgatgc gaacggccag atcagtctgc
    17221 ccatcggggg gatcatcaag gccgacagct tgaccattga gcagctggcg gacaagattg
    17281 tagaaaggat ttctctctac aatcgtagaa tcaccaacgt atccatcaag gtgaccgagt
    17341 atggcagccg gaaggtctat gtgatgggcc acgtgaaaga tcccaaaaaa tatacctttg
    17401 agatcatccc caatctgtgg gatatcattt ctgaagccgg tggacccctg gagactgctg
    17461 atctgagtaa tgtgctgatc attcgtaaat cggccgacgg ggatccccgg accatgtcgg
    17521 tggatgtctc tgaagtcctg cgcaaccggg aattccagcg cttgcccaaa atcgagcccg
    17581 gggataatat ctatgtttcg gccatcgtgg gtgcagctgc cagtaccggc atacaggcca
    17641 tgcagacgca gccgaatgtg cttttcatct atggtgaggt cggcaactcg ggtgttttca
    17701 ctttcaacaa agagctcaat ttgcttgagg ccttgattac cgctggaggg ccgacctcta
    17761 cagccaagct gagcgaggtg cgggtgatac ggaaaaacgg tgcctactcc agcgtcacca
    17821 aagtcaatgt aaaaaagtat gctgatgaaa gctcaccctc ctttttcctt gtcaaaggcg
    17881 gggacaccat ttatgtaccc agaaaaagcg tttttcgcga aagtgtgttt tgggatatta
    17941 tcatgatttt ttccggtgca gcgctgacct cgctagccta tagcctggtc accgcagagg
    18001 actaaaccgt tatcgatgag gcaacaaaca tgtcaacccg taaaggcatt gactggcaaa
    18061 tgtggtggca gctgcttttg cgacgccgct actacatcat catcagtatg ttcatcgccc
    18121 tagccggcgg tctctatctt gctttgacga cggttcctgt ctaccaatcg gcctccactg
    18181 tgatgattgt tgacgccaat ctgctctcag gcgcctcgtt gcgttttgtc ccctccacct
    18241 cacaggttaa cgaaatcgac tacttccgtc ggcgaatcac cagcgaggga tttctgctcc
    18301 gccttctcga cagcctcgag gtcgagcagg atcccaaagt agtcctgaaa attgaccagc
    18361 tctgtgcgca gaatcccttc atcgaacgcg aggtgattgc caagcaggtc tatattgaac
    18421 atctcttcaa gcggatttca acacgaatga aggcctataa tctcatcgaa ataagcgccg
    18481 aagggccttc gtcagaggag gcctttcgac tggcgacgct ggtgacagag ctggcgatcg
    18541 ccgagtccca gcagagtcag atacaatcgg tctccgcggt cagcagtttt agcagccaac
    18601 aactcgacat ctatcgcaag cggctcgagg acaccgaaaa ccgcctgaat gccttcaaga
    18661 cctccctcac ggtccagggt ttgagtgaga atgaactgag ccgcgagaag ctgtccgaga
    18721 tacaatcggt gaaactgtcc accgatatcg atttgcaggc caagcgcgaa caaatgcaaa
    18781 agctactttc cggatctctt cgcgatatca ccctgtccta cagaagcgat ctggatcaga
    18841 ctacccgcga tttgcgtcag cgtgtgctca agcgcaccag ggatgtatgc gagttgctta
    18901 aaaagttcag ttggcgggat gttgagatca tcctccttaa cgaagagatc ggccaattga
    18961 aggagcaaat ctatgagtcc atcaggaacc gagtcacggt cgatctgggc acgaaaagct
    19021 ctactgtggt cgatgctgcg atcaaattgg aagagctccg gcttgatgtg gatattttac
    19081 agaactcgac agaaattctg caagagatca tcgccagcca taatgacttg atcagacgcc
    19141 aaccttctca agagggactg cgtgccaaac tggaaaggga agtgcagatc aatcgcgaga
    19201 tttatgaagc actgctgcag gcaaaccagg gatcacaaat gcgcgagtcc gctgaggtca
    19261 ctgagaccaa gatgagattc aaactacttt ctcccccgca aaggcccctt gaacgtatca
    19321 aaccccaacg cgcgcggatg atgttcattg ccctgttttt aggtttgggg cttggcctcg
    19381 gtatggtgat ggctcgagag tacatggata ccagtttgac ctccatcgat gatgttacca
    19441 attatttcaa aatacccgtt cttgccgcaa tcccgaaaat tgagacccgg cttgaattga
    19501 cacgccaaaa gcatcgacgg tacgcagccg ctgttgccat acccctcatc gccctggttt
    19561 cggttatgct catctatcgt atctttattc gctaaaggat aaagcaatga agcagaatgg
    19621 cactctcaag ggcatcagcg aatattctga gcattccccc tattttaccg agttttatcg
    19681 actgtatgca cgaatgcgtg cgccgattga aggacggatc ccgcagatct tcatggtgac
    19741 cagtgccatc gcaagtgagg gcaagaccac catttccgcc tatattgcgc tcacggccgc
    19801 cctctcgacc aagagcaatc atctggtcat ggatactgat ttgaatcgcc cgacgttgca
    19861 caagaaactg ggactggaac aggtcaatgg cctcacagag attctcagtg atggattgga
    19921 actccagcaa gcgattcaaa ctacacccta ttccggcctg catgtcatca gcgccgggcg
    19981 taatgttgcc cgtcccttcg agctgttgag tttcggcaga gtggagaatt tattcaacca
    20041 gctgcgcctc ttctatgata ccatcattct ggatgcaccc cccattttgc atgtgggcga
    20101 cacactcaaa ctggccgagt atgtggacgg tattctgctc gtcgtacttt ccggccgcac
    20161 aaaccgtgaa atcgtcaaac gggccatcga gctgcttgac gagaccggca agccgattct
    20221 gggtatcgtc atgaatgata tgggtgaagt attgccctat tattatcagc acaagtatta
    20281 ctcctatcat tatggttctt ctgaagtcaa gtgaacagca ggaggagtga tggcttcagc
    20341 caagcttgct cgcagtatcg ctttcaatgt cccctgcatc ggggctgagg agatcggcag
    20401 tgtagtggag accttgcgtt caggatggct gacgacgggt tcaaaagctg cccaactgga
    20461 aaaagaagtg gccgccttca ccagagcccg ttatgccgtg gccgtcaact cctgcacggc
    20521 cggtttacat ctctccttgc tggccctcgg ggtcgggcct ggtgatgagg tgatcacgac
    20581 tccctacacc ttttccgcaa ctggtgagac cattgtccat acgggggcga gacccgtgct
    20641 ggtggatgtt gaacgggggg gattcaacat cgatccccaa aagatcgccg cggcgatcac
    20701 ggcccgaacc aaggctatcg tgcccgtcca catcgccggt gatccctgca atatgacgga
    20761 aatcctgagc ctggcccggc gaaagggtat ccatgtggtc gaagatgccg cgcatgccct
    20821 cgggacctgg tatgacggga atccggtggg gagcatcagc acgctgaccg ttttcagttt
    20881 ttacgccaca aaaaatctga ccactggcga aggtggcatg gtcaccaccg attcggagaa
    20941 gctggcgaag aacataagac agctcagtct gcacggtctt tcgcacgacg cctggaatcg
    21001 ttactctgac cggggaagct ggcgatatga aatctgcact ctgggctaca agtgcaacct
    21061 ttctgacatc caggcggcca tcggcattgc ccaaatgcac aaattcgaga ccatgcaggc
    21121 acgccgccgc aaactggttg aggcctatca ggagggcttg gccggactcc ctgatctgat
    21181 cctgcccgag gagagggagg ggaccaggca tgcctggcac ctctatatca ttcgtctggc
    21241 tgaggagcgg gcgcgggagc agcgggatga tttgattgaa cagcttaaag gaatgggcat
    21301 aggagtcagt gtccatttta tccccctgca tctgcatcgc tactatcaac agactttcaa
    21361 ctaccggcct ggtgattttc cggaagcgga gaagcagtac caggcggcaa tttccttgcc
    21421 tctttacccc acattgcggc gggaggaagt cgcttttata tgtcgtgctt tgcgtcgtct
    21481 cttgcaccga ccggcgccac atcgatcaag gagggggatt agccaccatg ataaagcgat
    21541 tgtttgacct cgtatttgct tttttgggac ttttaatatc tatccctttg ctggctgtgt
    21601 tggccgtg
//
LOCUS       DAOPWU010000021        21482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_2438_length_21482_cov_4.717413, whole genome shotgun sequence.
ACCESSION   DAOPWU010000021 DAOPWU010000000
VERSION     DAOPWU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21482
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2438_length_21482_cov_4.717413"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..972
                     /gene="bamD"
                     /locus_tag="PKM23_02430"
     CDS             178..972
                     /gene="bamD"
                     /locus_tag="PKM23_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNY90358.1"
                     /translation="MKPFARLFVVFILGVILAAASGCGGTKMRANMTLEERMAAAMEL
                     YEKRDYLDAKNQFRVITLSYSGSTVADKAQFYLGECHYGMKEYILAASEYERLLKVYP
                     NSEWVDDAKFKLGMSYFKLSPKYSLDQDYTHKAIREFQEFLEEFHRSDLVKQVQEKLT
                     ECREKLALKVYSSAEQYYKLGYYDAAAVYYNVVLDEHYDSKFSPMAQYYLGECYRHMR
                     KPVEALEAFRKMADKFPNHELAGKARQRITQLEQAAPAAGAAGGKP"
     gene            986..1576
                     /gene="nadD"
                     /locus_tag="PKM23_02435"
     CDS             986..1576
                     /gene="nadD"
                     /locus_tag="PKM23_02435"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNY90359.1"
                     /translation="MKKLALFGGSFNPVHLAHLIIAERAADALDVDRLLFMPCSVPAL
                     KPDDQMVAGEHRLAMVRLAAAGNPRFRASDLELRRGGRSYTVETLTELKKKHGLDRSG
                     LFLVIGGDNLADLLSWRAPEEINRLCRIVVADRPGISYVGRVPGFIRDLIIVDTPLMH
                     LSSTDIRKRLLEGRSIRYLVPPAVERYIARHQLYRG"
     gene            1590..2756
                     /locus_tag="PKM23_02440"
     CDS             1590..2756
                     /locus_tag="PKM23_02440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY90360.1"
                     /translation="MTGRTLHLAQICSSRSWGGLEMHVAVLSCWLAEEGHRVEIWCAP
                     GSPLANEAQRLGLPVIPFAPAGYLDLAASLRLFHQFREQEVELLHAHYARDLWNIAPA
                     VHLGRRWPLVFIKHIGTGKPKRDPIHRWIYAAVDQVVAISRVIEENLLATHPLNRERV
                     CVVHHGVDTRTFAFRPEARARIRAEWGVGEEELLIGSIGRLQPGKGHLEFLDMAAAVG
                     ARHPRARFVIVGEPTRGEEERARPIHDRLRSLGLEGRVIMAGFRSDIPEVLSAMDIFA
                     FPSRAEAFGLVLIEAMAVGRPVVSTRCDGVLDIVEEERNGLLFNPAEPGDLTRQVERL
                     IVDEELRLRLGRGGLETVAARFNRAAMLNRLYAVYERAGARRGVQLGPLSAARS"
     gene            2843..3535
                     /locus_tag="PKM23_02445"
     CDS             2843..3535
                     /locus_tag="PKM23_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNY90361.1"
                     /translation="MAEERVIRLLKRVPLFAKMSDADVEQVADRCLRKDCHKEQVILV
                     EEEVGQTLFIILRGTVKITRASDEGREVIITILKAGDFFGELSILDGKGRSASVIAME
                     ESELLTLRRNDFHHLLERFPSIGLEMLRVLADRIRKADAMIENLTLQDAVGRVGSTLI
                     HVAEQTGYARGASMIIAKLPVQQDLANMAGTARETISRVMAVFESKKLIERDGHRVII
                     PDFEKFKRDYWS"
     gene            3645..4280
                     /locus_tag="PKM23_02450"
     CDS             3645..4280
                     /locus_tag="PKM23_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNY90362.1"
                     /translation="MSVLEMLAESVISGNASRAEELTLTALGEGIEPSEILNKGLIAA
                     MDIVGVKFRNNEFYVPEMLVAARAMKAGIKVLRPKLVETGVKPIGRFVIGTVKGDLHD
                     IGKNLVSMMMEGAGFEVIDLGVDVAADKFVAAIREHKPQVVGLSALLTTTMLNMKDAI
                     SAFEAADVRDQVKVVIGGAPVTPQYAEEIHADGYAADAASAVDLVKGLLPA"
     gene            4305..4946
                     /locus_tag="PKM23_02455"
     CDS             4305..4946
                     /locus_tag="PKM23_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNY90363.1"
                     /translation="MRGEAKAGAPLRVIFFDLGGVLVNVHFERFIEGLARQTGRTPAE
                     LIALRASMRSDSRLFDSGGIDGDEFLERLNRKLDRPLERPLLESLYTDIFSLKEDVAG
                     LARLLHGRFRLSIISNTDPLHYNYIVGRYPFFSLFEKPVTSFGAGLLKPDPAIYAFAL
                     RELAVEPEEALFIDDLEENVAGARQAGMRVLHFSGAETLRLQLDGLLPPGQGQ"
     gene            4998..6224
                     /locus_tag="PKM23_02460"
     CDS             4998..6224
                     /locus_tag="PKM23_02460"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNY90364.1"
                     /translation="MSLAINGGTPVRTMPFPVWPVWGDEEIEGLTRVIKSGKWGRLAG
                     KESECFEKQFAEAHAAKHGIAINSGTTALRIALTAADVDAGDEVIVPAYTFIATASAA
                     VEAGAVPIFVDIDRDTYNIDVRLIEAAITPRTKAIMPVHFAGRPADMDAIMKIAARHH
                     LVVIEDAAQAWGSAWKGRPVGAIGAAGCFSFQSSKNINCGEGGIILTNDDLVAKFARS
                     HHNCGRSEDGLWYEHFYFGGNYRITEFQSAVLLAQFGRYEELKAIRRANLDLLNELLG
                     GIEGISPLPMQEEVTSHSCHLYIFRYDKRAFASKSKQLFIDAMRKEGIPTSPGYSIPL
                     YKQPVFMNKSFGPRGRKVELPVDYASNFCPESEKACYEEAIWFTQSVLLGTEEDMQDI
                     AAAVRKIQQHADELKG"
     gene            6360..6725
                     /gene="queD"
                     /locus_tag="PKM23_02465"
     CDS             6360..6725
                     /gene="queD"
                     /locus_tag="PKM23_02465"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNY90365.1"
                     /translation="MYQLVVRSGFSAAHRLAYYPGACERLHGHNWKVTATVAGEELDQ
                     LGMVMDLMVLQTILDECLHQFDHRMINEVAPFDQTNPTSENLARYIFGWMKNNLPAKA
                     RVVSIEIAENDDFSVIYSE"
     gene            6767..7177
                     /gene="queF"
                     /locus_tag="PKM23_02470"
     CDS             6767..7177
                     /gene="queF"
                     /locus_tag="PKM23_02470"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNY90366.1"
                     /translation="MDELKRGQREILEAKLEVFENRYPGRDYVITISDPEFSCLCPRT
                     GYPDFATVEIRYVPDQYCLELKALKLYINKFRDRGIFHEEAVNEILEALVEVCRPRWM
                     AVHGDFNPRGNIHTRVDAEYKAPGFDKKRLASGY"
     gene            7372..7632
                     /gene="pheA"
                     /locus_tag="PKM23_02475"
     CDS             7372..7632
                     /gene="pheA"
                     /locus_tag="PKM23_02475"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNY90367.1"
                     /translation="MNLSSLRQEIDRIDEQIVDLISERATLAQEIGTLKGSTGMTVRD
                     KQREEAVLYHIQSHNQGPLSNDEMVAIFKRIIHACRELQFLA"
     gene            7661..8674
                     /gene="aroF"
                     /locus_tag="PKM23_02480"
     CDS             7661..8674
                     /gene="aroF"
                     /locus_tag="PKM23_02480"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNY90368.1"
                     /translation="MVIVMKDSASEGMVQAVCEKLEAHGFQVHKVVGVNQTILGGIGD
                     IRGVDLREFEVMEGVYEVLRITEPYKLASRAFHPENTVVEVGEASFGGEMVTVCAGPC
                     SVESEEQLFTIAREVKKCGAKVLRAGAFKPRTSPYAFQGLGEKGLQLMQQVGAELSIA
                     TVSEVMEIGQLDLMLRYIDIIQVGARNMQNFALLRELGHIRKPVLLKRGLSATIEELL
                     MAAEYIMSGGNYNVILCERGIRTFETYTRNTMDISAIPVTQKHSHLPIIADPSHGTGR
                     RDKVAPMARAAVAAGANGLIIEVHHDPDHALSDGAQSIYPQQFMRLMEELRMIAWAIG
                     KRI"
     gene            8693..9802
                     /locus_tag="PKM23_02485"
     CDS             8693..9802
                     /locus_tag="PKM23_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNY90369.1"
                     /translation="MEEKLAFKEIAIVGVGLIGGSLGLALKRKGYQGVLTGVDNQEVL
                     ALARQRGAIDKGFETADIADALATADLVFLCTPVANILHHLQTIGSCVRPGTLITDVG
                     STKRRIVETANIHLPSQCDFIGGHPMAGSEFKGMEAADAFLFENTTWVLTPAKPMREE
                     RRKAIGELVEMTGALVLLISPHLHDEIAAAVSHLPQMAAIALMSLAAQRQSESSHFLK
                     MAAGGFRDMTRIASSPFSVWEDIITTNADMIGSYIDAYIEELKRVKTALDKKEMRGIF
                     DQSARNRLSIPRDTRGFFRPHFDISVVVQDRPGMLATIAGALLEKNINIKDIEILKIR
                     ENEGGTMRLAFASEVDREMALALLKSRGLECRKRE"
     gene            9804..11090
                     /gene="aroA"
                     /locus_tag="PKM23_02490"
     CDS             9804..11090
                     /gene="aroA"
                     /locus_tag="PKM23_02490"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNY90370.1"
                     /translation="MSDILVKPARTVQGTFSVPGDKSISHRALILSAMAEGKSKITGL
                     SDARDVASTRRCLENLGVKISSLKGGIVAVAGKGKYGFRAAKGPLDAGNSGSTIRLLS
                     GVVAAQPFTTTITGDESLCRRPMRRIIEPLERMGAHIESSAYKAPLVIRGGALRSIDY
                     ASTIASAQVKSCILLAGLYANGTTRVTEPSPSRDHTERMLEAFGVKAHFSQSGAGVQG
                     PATLQACDIDVPGDLSSASFFLVAASLLADSEITIEQVSVNPTRMGIYDALSAMGAMI
                     QTSPAVTFQGEPRADLSVRSARLHSAALGGSMIPRIIDEIPVLAVAATQAQGTTVIRD
                     AGELRLKESDRLTAICRNLQAMGANVRETKDGMIITGPTSLHGAEIESYDDHRIAMAF
                     AIAGLIATGETLIKNGSCTEISFPGFFDLLDRIRHD"
     gene            11083..11955
                     /gene="aroE"
                     /locus_tag="PKM23_02495"
     CDS             11083..11955
                     /gene="aroE"
                     /locus_tag="PKM23_02495"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNY90371.1"
                     /translation="MISTRTRLFGLIGDPVSHSLSPIMQNHLFKLFGLDGVYLAFRTA
                     GERLAEAISGARALALAGLNVTIPHKSAVVPLCDYRSPEVELLGVANTLVFRDGRIHA
                     FTTDHSGFVDSLGGEKSRFSGSRVLLFGAGGSARAVAFALARLGIARLTIVNRSRTRG
                     EELAEFCSRELAISAVEAISPLDPVLGGKITEAAILINATACGMFPHIEESPLSAFDL
                     IDRRHFIYDLVYNPRETRFMRESGARGAAVQGGLDMLIFQGLAALNHWFYSDYRLDPA
                     EMKKLKSLLNRELA"
     gene            11955..13163
                     /gene="aroC"
                     /locus_tag="PKM23_02500"
     CDS             11955..13163
                     /gene="aroC"
                     /locus_tag="PKM23_02500"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNY90372.1"
                     /translation="MEKLRFLTAGESHGQGLVAILEGIPAGLRLSAADIAPDLKRRQR
                     GYGRGGRMQIEKDTVRILSGVRYGKTLGSPIAMFMENLDWPNWTAKMALEEPVEPVAA
                     LHMPRPGHADLAGMIKYRQEDLRNILERSSARETTARVAAGAVARRLLAAFGIEVYSH
                     VLSIRGIQGGITAAGFFKGRTSRDPELLASWRALMERVESSPLACADAEAETEMIAAI
                     DGAREKGDTLGGVIEIAALGLFPGLGSHVHWDRRLDGLLAGALMSIPAIKGVEVGLGL
                     GVTLKPGSQIHDEIFHESGQGFYRGGNNAGGIEGGMSNGEPLILRVHMKPLPTLARPL
                     RSVDVATLQPQSAHHERADVCAVPAAAVVAEAMTALVLADALCVKLGGDSISEMQQHY
                     ASLAHVPLGW"
     gene            13177..13680
                     /locus_tag="PKM23_02505"
     CDS             13177..13680
                     /locus_tag="PKM23_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNY90373.1"
                     /translation="MGFMGTGKSKVGRAFARLLGRPFADSDDLIVARAGMPVEQIFKQ
                     RGEEEFRVVEDEIVDEISRRSQWVISLGGGAVLRQANWEKLQTSGLTICLEASLEVLA
                     SRLARKNDRPLVRGLSGEALHNRIQELYSARAQVYARANYHFQSREEVSAAELAEEIF
                     LYLRDEP"
     gene            13677..14783
                     /gene="aroB"
                     /locus_tag="PKM23_02510"
     CDS             13677..14783
                     /gene="aroB"
                     /locus_tag="PKM23_02510"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNY90374.1"
                     /translation="MRSVRVELQRMVSPIHIGFGLLERMPELLARHNLQGELVIISDE
                     RVAGLYGERLLTALGAAGRSAHFLRVPEGEGAKSLEQCEVLWRKLISGGFSRSTIILA
                     LGGGVVGDLAGFVASTFLRGVRLVQIPTTLLAQVDSSVGGKTGINHPLGKNLIGAFHQ
                     PELVCIDPALLATLERRDRLAGLGEVAKYALIRDRALFTLLALNLEALTELAYPELCE
                     SIIETSCTIKAEIVSADERERGERVLLNFGHTIGHALEAAGGFSHFRHGEAVIRGMAG
                     AIWLSRRIGRLSRAEAQTALDLLTRLAPPAPPPGLRPADLLAWMARDKKRGPEGQTWI
                     LLEGVGKAFITREVAEDLLPPALAFACAGEWRAP"
     gene            14849..15292
                     /gene="aroQ"
                     /locus_tag="PKM23_02515"
     CDS             14849..15292
                     /gene="aroQ"
                     /locus_tag="PKM23_02515"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017288126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNY90375.1"
                     /translation="MFTVLVIHGPNLNLLGEREPEQYGRRTLAELNRELEEEAAKLGV
                     QLEILQSNHEGVLIDFIHAQRRNAAGILINPGALTHYSYALRDAIAGVNLPAVEVHLS
                     DIHQRESFRRISVIREVCLAQISGRGFDSYLQGLHLLVDYIRKNG"
     gene            complement(15374..15508)
                     /locus_tag="PKM23_02520"
     CDS             complement(15374..15508)
                     /locus_tag="PKM23_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90376.1"
                     /translation="MNRANTSARDGVFSGAGIDYFAVITPEMHSTFLPAIKGRLEAPK
                     "
     gene            15596..17497
                     /locus_tag="PKM23_02525"
     CDS             15596..17497
                     /locus_tag="PKM23_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90377.1"
                     /translation="MFVLAGIPGLLAGAGIERPFDPVVVTGAQLGDFLGADTSDIYVY
                     AWVEGSWRQIPFQIDQVDWNSELAEYSYFAPHDAVLDSLDELCFMAVDMGDSVADNRW
                     IGDIGSTLYQRYQVAAWDTSVVPARKSYAYVYRSSSITPAFSSYMTYETGVVGVSDTV
                     SAQSYIYGQNADAIPDFLSLLTDEGRGPDILDRWKIRFKGSIGPYWGTPEYIESEESA
                     LRDPALQVRAGAVRVIHRREFGINMQGNELPLYLDLESVFYPWSYKINLAKEGLPGYL
                     GMQEMRQTVDYLPNITGSLFHWSGSTGIPVDGALDIIPDKSFDTPAVNWYMVQGEFGM
                     AATVFNMLPITGTQLSLYYIDDSSTEHGAERDGIGDTGDMMAYAESGVQLTASGSDPI
                     DIEDDDENLAATFYYLPGYHTAAYGDSLADYTRHPLRIVVTPRTNTVIPVEMTALKAQ
                     LVKGSVRLEWTTVSESNNYGFDVERRGAAESSWSKIGFVKGNGTSQIAHAYQFMDKEP
                     ATGSNSYRLKQIDFDGRSTYSQEVAVDIQLPERLELGQNYPNPFNPGTGITFQLPAGE
                     QQRVTLAVYNLLGQKIRTLVDSNLEPGTHQVYWDGRDDRGLNAVSGAYLYRLQLGAQV
                     LTRKMIKMQ"
     gene            complement(17585..18745)
                     /locus_tag="PKM23_02530"
     CDS             complement(17585..18745)
                     /locus_tag="PKM23_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNY90378.1"
                     /translation="MEHIQKNPAAGPAKGGEKETLFRMDFNQYFLLFSLLAVLVLFVF
                     MIKIFVVPIIVAAVFATLFDPLFKRFVRWTKGRTALSSLLTCLVLLLVLLIPLYVLVN
                     IVINQAINLYDSIGPALEEFYNSGDFNLESLTSHPILAKFKLDQIDWQATIKEGITTI
                     STYAGKVINATSRGTFAFLTNLIMILFTMFYFFKDGDRIIAQVMGFLPLEDDYKQKIV
                     SRFSAISRATIKGSAILGLIQGTLGALTMWIFGFKAIVLWGMVMVILSLLPMVGAYMV
                     LIPAAIYKIAMGEAAAGIAILLISVLVISNVDNLLRPRLVGRDAGMHDLMVFFSTLGG
                     IGFFGIMGFIIGPVVASLFITLLDIYSTEFKRQIRRQKSSAQIPDAALPPAE"
     gene            19025..20194
                     /gene="sucC"
                     /locus_tag="PKM23_02535"
     CDS             19025..20194
                     /gene="sucC"
                     /locus_tag="PKM23_02535"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNY90379.1"
                     /translation="MKIHEYQAKELLARYGIPVQRGAVAFKPREVRRLAEKMALKEIV
                     LKAQVHAGGRGKAGGIKLLHSPAEAEDAAAKMFGMRLVTPQTGAEGKIVRRILVTEAA
                     EISRELYAGIVLDRARRQLVFMVSQAGGVEIEKVAEESPEKIIKEYVDRAAGLQPFQA
                     RRMAFALGLTGPLFREGVRLLLALYEACIQCDASLLEINPLVVTPDQKLLALDVKLNV
                     DDNALFRHPDIQEMRDTGEEDPLEVEASSSNLNYIKLDGNVGCMVNGAGLAMATMDII
                     KLAGAEPANFLDVGGGASAETVENGFRIILSDRNVRAVLINIFGGIVRCDRVARGVIE
                     ASKKIGVTIPVVVRLAGTNAEEAALLLQQSGMNFGVARDLAQAAELVVAALNKPN"
     gene            20210..21100
                     /gene="sucD"
                     /locus_tag="PKM23_02540"
     CDS             20210..21100
                     /gene="sucD"
                     /locus_tag="PKM23_02540"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNY90380.1"
                     /translation="MSILVDKKTRVVVQGITGGEGSFHAAQMIEYGTQVVAGVTPGKG
                     GQLFEKRVPIYDTLAEAVREQKADASIVFVPAPFAADAVMEAADAGVKLVVCITEGIP
                     VVDMLRVTEYLAKRPTRLIGPNCPGIITPGGCKLGIMPGFIHKPGRVGVVSRSGTLTY
                     EAVGQLTNLGLGQSTCIGIGGDPVIGTTFLDALQLFEADKKTEAVVMIGEIGGTAEEE
                     AARYIEEKMTKPVVSFISGQTAPPGRRMGHAGAIIAGGKGTAAEKMEALAEAGAAVCS
                     SPADIGRMVKKVLTARRNMA"
     gene            complement(21235..>21482)
                     /locus_tag="PKM23_02545"
     CDS             complement(21235..>21482)
                     /locus_tag="PKM23_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNY90381.1"
                     /translation="RFRVLSTVMYAVMGWSFLIAYNQIITRMPPLSTRFLLYGGLAYT
                     VGILFYVWKKQPYAHSIWHLFVIAGSILHFFAIYYLL"
BASE COUNT         4253 a         6758 c         6377 g         4094 t
ORIGIN      
        1 cctcgcacgc ggccagatcg cagcggtcga agatccggcc ctccctttca ccatcggcaa
       61 gaggccgcag ccctcccggc tcaagaggat ggccgaaccc gtcgtcatgg ccctgatcac
      121 cggcgggatt atttaccttt tttactcttt tcgcagcaac tgaactacag gaaccgtatg
      181 aaaccctttg cccggctttt tgttgttttt attctcggag tcatactcgc tgcggccagc
      241 ggctgcggcg ggaccaaaat gcgcgccaac atgaccctcg aagagcgcat ggccgccgcc
      301 atggaactct atgaaaagag ggactatctg gatgccaaaa accagttcag ggtgatcacc
      361 ctgagctaca gcggcagcac tgtcgccgac aaggcccagt tctatctcgg cgaatgccat
      421 tacggcatga aggagtatat cctcgccgcc agcgaatacg agcgcttgct caaggtctat
      481 cccaactccg aatgggtgga tgacgccaag ttcaagctgg gtatgagtta ttttaaactc
      541 tcgcccaagt actccctcga ccaggactat acccacaagg ctatccgcga attccaggaa
      601 tttctcgaag agttccaccg cagcgacctg gtcaagcagg tccaggaaaa gctcaccgag
      661 tgccgggaaa agctggcgct caaggtctat tcctcggccg agcagtacta caaactgggc
      721 tactatgatg cggccgccgt ttattacaac gtggttctcg acgagcatta tgactccaaa
      781 ttcagcccga tggcccagta ctatctcggc gaatgctacc ggcacatgcg caagcccgtt
      841 gaggccctcg aggccttccg gaagatggcc gacaagtttc cgaaccacga gctggcgggc
      901 aaggcgcggc agcgcatcac ccagctggag caggccgctc cggccgcagg cgccgccggg
      961 gggaagccct gatttgaagc gggaggtgaa aaagctggcc cttttcgggg gttcattcaa
     1021 tccggtccat ctcgcccatc tgatcatcgc cgagagggcc gccgatgccc ttgatgtcga
     1081 tcgcctgctc ttcatgccct gttctgtgcc cgctttaaag ccggacgacc agatggtcgc
     1141 cggggagcat cgcctcgcca tggtccgtct tgctgctgcc ggcaatcccc gcttccgcgc
     1201 cagtgatctt gagttgcggc gcggcggcag gtcctatacc gtcgagaccc tgaccgagct
     1261 gaaaaaaaaa cacgggctcg accgctccgg gctctttctc gtcatcggcg gagataatct
     1321 cgctgatctg ctctcgtggc gggcgcctga agagatcaac cggctctgcc ggatcgtcgt
     1381 agccgaccgc cccggcatct cctatgtagg ccgggtgccg gggttcatcc gggacctgat
     1441 catcgtcgac accccgctca tgcatctttc gtcgaccgac atccgcaaac gcctgctgga
     1501 ggggaggtcg atccgttatc ttgtgccgcc cgccgtcgaa cgctacatcg cccgccatca
     1561 gctctacagg ggctgaggga gcgggattga tgaccggtcg caccctgcat ctcgcacaga
     1621 tctgctcctc gcgctcctgg ggtgggctgg agatgcacgt cgccgtgctc tcgtgctggc
     1681 ttgcggagga ggggcacagg gtcgagatct ggtgtgctcc gggctcaccc ctcgcgaatg
     1741 aggcgcagcg tctgggactg cccgtcatac ccttcgcccc ggccggctat ctggatctgg
     1801 ccgcgtcgct ccgcctcttt catcaattca gggagcagga ggtggagctg ctgcacgccc
     1861 attacgcccg ggatctctgg aacatcgccc cggccgtcca tctggggcgc cggtggccgc
     1921 tggtcttcat caagcacatc ggcacgggca agcccaaacg cgatcccatc caccgctgga
     1981 tctacgccgc agtcgaccag gtggtggcga tctcgcgggt catcgaggag aatctcctcg
     2041 ccacccatcc gctcaaccgg gagcgggtct gcgttgtcca tcacggggtg gacacccgca
     2101 cctttgcatt ccgtcctgag gcgcgcgcgc gtatccgggc tgaatgggga gtgggggagg
     2161 aggagctttt aatcggctcc ataggtcgtc tccagcccgg caaggggcac ctcgaattcc
     2221 tcgatatggc tgctgcggtc ggcgcccgcc atccaagggc ccgatttgtg atcgtcggcg
     2281 agccgacccg tggagaagag gaacgcgccc ggccgatcca tgaccgtctg cgcagcctcg
     2341 ggcttgaggg gcgcgtcatc atggccggct tccgcagcga cattccggaa gtgctgtcgg
     2401 ccatggatat tttcgccttc ccttcgcggg ctgaggcctt cggactggtg ctgatcgagg
     2461 ccatggcggt aggccgtccg gtggtatcga cgcgctgcga tggcgtcctc gacatcgtcg
     2521 aggaggaacg caacggcctg cttttcaacc ccgctgaacc cggggatctg acccgccagg
     2581 tcgaacggct gatcgtcgat gaagagctgc gtctccgtct cggccgcggc ggcctcgaaa
     2641 cagtggcggc cagattcaat cgagccgcca tgctcaaccg tctctatgcg gtctatgagc
     2701 gggcgggagc acgccggggg gtgcagcttg ggcccctgtc ggccgcacgg agctgagccg
     2761 gttggcttgc atcggcccct tttaattatc acacggctgc tttacgccga atttgcgcca
     2821 tcacacgacg ggaggagtcc atatggcgga ggaaagagtc attcggctgc tgaagcgggt
     2881 gcccctgttc gccaagatgt ccgatgcgga tgtcgaacag gtcgcggacc gctgtctgcg
     2941 caaggattgc cacaaggagc aggtcatcct tgttgaggag gaggttgggc agaccctctt
     3001 tatcattttg cgcggtacag tcaagataac ccgagcgagc gacgaggggc gcgaagtgat
     3061 catcactatc ctcaaggcgg gcgatttctt tggcgagctc tccatactcg acggcaaggg
     3121 ccgatcggcc tcggtcatcg ccatggaaga gtccgagctg ctcaccctgc gccgcaacga
     3181 tttccaccac ctgctcgagc gctttccctc catcggcctg gagatgctgc gcgttctcgc
     3241 cgatcgcatc cgcaaggccg acgccatgat cgagaacctg accctgcagg atgccgtcgg
     3301 ccgggtgggc tcgaccctta tccatgtcgc cgagcaaacc gggtatgcgc gcggcgccag
     3361 catgatcatc gccaaactgc ctgtgcagca ggatctcgcc aacatggcgg ggaccgcccg
     3421 tgagaccatc tcgcgggtta tggccgtctt tgagagcaaa aagctgattg agcgcgacgg
     3481 tcatcgggtc atcatccctg atttcgaaaa attcaagcgc gattactggt cctgaaccgg
     3541 caggaaaagg ggccggactg ctggcgcgat cgttccttat cgccggggtg caagaccccg
     3601 gccgggctgt tattttgaga acaatatcat cctcggagga gtacatgtcg gttctggaaa
     3661 tgctggctga aagtgtcatc tcgggcaatg cgagccgggc ggaggaactc accctgaccg
     3721 cccttggcga gggcattgag ccttcagaaa ttctcaacaa gggcctgatc gccgccatgg
     3781 atatcgtcgg cgtcaaattc cgcaacaacg aattttatgt cccggaaatg ctcgtcgccg
     3841 cccgcgcgat gaaggccggc atcaaggtgc tcagacccaa gctggtcgag accggggtca
     3901 agcccatcgg ccgctttgtc atcggcacgg tcaaggggga cctgcacgat atcggcaaga
     3961 atctggtttc aatgatgatg gagggggcgg gctttgaggt gatcgacctc ggcgtcgacg
     4021 tcgcagccga caaattcgtc gcggcgatcc gggagcacaa accccaggtc gtcggcctct
     4081 cggccctgct gacgacgaca atgctcaaca tgaaagatgc catctccgcc tttgaggcgg
     4141 ccgacgtgcg cgaccaggtc aaggtcgtca tcggcggcgc cccggtgacc ccgcagtacg
     4201 ccgaagagat tcacgccgac ggctacgccg cggatgcagc cagtgctgtc gatctggtca
     4261 aggggctgct gcccgcatga ggaatccatg ggcggcgaag tgatatgaga ggagaggcca
     4321 aggctggcgc accgctcagg gtcatctttt tcgacctcgg tggcgtgctg gtgaacgtcc
     4381 atttcgagcg gtttattgag gggctggccc ggcaaacggg ccgtaccccg gccgaactca
     4441 tcgccctgcg ggcgagcatg cgctccgatt cccggctttt cgacagcggc gggatcgatg
     4501 gcgatgagtt tctcgagcgg ctcaaccgca agctcgaccg gccgcttgag cggccgctgc
     4561 tcgaaagcct ctataccgat atcttttccc tcaaggagga tgtcgccggg ctggcccgct
     4621 tgctgcatgg ccgtttccgc ctctccatta tcagcaacac tgatccgctg cattataact
     4681 acatcgtcgg acggtatcct tttttcagcc tttttgaaaa gccggtgacc tcctttgggg
     4741 ccggtttatt aaagcccgat cccgccatct acgcctttgc cctgcgtgag ctggcggtcg
     4801 aaccggaaga ggcgctcttt atcgatgatc tggaggagaa cgtcgcgggg gcccggcaag
     4861 cgggcatgcg cgtcctgcat ttctccggag cggagaccct gcggttgcag ctcgacggtc
     4921 tgctgccgcc cggacaaggc cagtgatttt tccgaacagt tgaaccatca aaaccagttt
     4981 taaagaggag gcgtacaatg tcgttggcga tcaatggtgg aaccccggtg cggacgatgc
     5041 cttttccggt ctggccggtc tggggagacg aggaaatcga ggggctgacc agggtcatca
     5101 agagcggtaa atggggaagg ctggccggca aggagagcga atgttttgag aaacagttcg
     5161 ccgaggccca tgccgccaaa catggcatcg ccatcaacag cggcaccacc gccttgcgca
     5221 tcgccctcac cgccgccgat gtcgacgccg gcgacgaggt catcgttccc gcctacacct
     5281 ttatcgctac ggccagcgct gccgtcgagg ccggcgccgt gcccatcttc gtcgatatcg
     5341 accgcgacac ctacaacatc gatgtccggc tcatcgaggc cgccatcacc ccgcgcacca
     5401 aagccatcat gccggtccat ttcgcgggac gcccggccga catggatgcc atcatgaaga
     5461 ttgccgccag gcaccatctc gtcgtcatcg aggacgccgc ccaggcctgg ggttccgcct
     5521 ggaaaggccg tccagtcgga gctatcggcg ccgccggctg cttctctttc cagtcctcca
     5581 agaatatcaa ctgcggcgag ggggggatca tcctcaccaa cgacgatctc gtcgccaaat
     5641 tcgcgcgctc ccaccacaac tgcgggcgca gcgaggacgg actctggtat gaacatttct
     5701 acttcggcgg caactatcgc atcaccgagt tccagtccgc ggtcctgctc gcccagttcg
     5761 ggcgctacga ggagctcaag gcgatccggc gggccaatct ggaccttctc aatgagctgc
     5821 tcggcggcat cgaggggatc tcaccgctgc cgatgcagga ggaggtgacc agccactcct
     5881 gccacctcta tattttccgc tatgacaagc gcgccttcgc cagcaagtcg aagcagctct
     5941 tcatcgatgc catgcgcaag gagggcatcc ccaccagccc gggctactcg atcccccttt
     6001 acaagcagcc ggtctttatg aacaaatcct tcgggccgcg cggccgcaag gtcgagctgc
     6061 ccgtcgatta tgcgagcaac ttttgccccg agagcgaaaa ggcctgctac gaggaggcga
     6121 tctggttcac ccagagcgtt ctgctcggca ccgaagagga tatgcaggat atcgccgcgg
     6181 ccgtgcgcaa gatccagcag cacgcggatg agttgaaggg gtagcaggcg cagccgcccc
     6241 tggcgccgcc ggacacggca cttcttccag cccggagggc ggcgccgttt ttaaaaacat
     6301 atctgcagag gcgccgtccg cccaggccgg cgctgtttat acctatcggg aggaagtgca
     6361 tgtaccagct tgttgtcaga tcaggatttt ccgcggccca tcgtctggcc tactatcccg
     6421 gcgcctgcga gcgcctccac ggccacaact ggaaggtcac cgccacggtc gccggcgagg
     6481 aactggacca gcttggcatg gtgatggatc tgatggtcct gcagaccatc ctcgacgaat
     6541 gcctgcatca gttcgaccat cgcatgatca atgaggtggc gccgttcgat cagaccaatc
     6601 caacctcgga gaacctcgcc cgttacatct ttggctggat gaagaacaat cttccagcca
     6661 aggccagggt ggtcagcatc gagatagcgg aaaacgatga tttctcagta atatacagtg
     6721 aatgaggggc cgaaaggtca aaggccccga tccgacggga gcagtcatgg atgagttgaa
     6781 gcgcggccag cgcgaaatac tggaagcaaa acttgaggtc tttgagaaca ggtaccccgg
     6841 ccgggattat gtcattacca tcagcgatcc cgagttcagc tgtctctgtc cgcggacggg
     6901 ctatccggat tttgccaccg ttgaaatccg ctatgtgccc gaccaatact gccttgaact
     6961 gaaagccctc aagctttaca ttaacaagtt cagggatcgc ggcatttttc acgaagaggc
     7021 ggtcaacgag atcctcgaag ccctggtaga ggtttgccgg ccgcgctgga tggcggtaca
     7081 cggcgatttc aatccgcgcg gcaatatcca caccagggtg gatgcggagt ataaggcgcc
     7141 cggctttgat aaaaaaaggc ttgcttctgg ctattaattt tgtaaattca ctatataaga
     7201 ctattgaacg gcaaacacga agcaaaatga acccttctcg ctacatcttt cccttttatt
     7261 accacctttt tggcggtcgg agcggataac aagcccggtg atcagttcgc cggcgtcatt
     7321 atccgcccac cagctcacac ctggtgggtt tttttttgga aggaaatgaa catgaatcta
     7381 tcatccctgc ggcaggagat cgaccggatc gacgagcaga tcgttgatct gatcagcgag
     7441 cgcgccaccc tcgcgcagga aatcggcacc ctgaagggga gtaccggtat gactgtgcgg
     7501 gacaaacagc gggaggaggc cgtgctttac catatccaga gccacaacca gggccctctt
     7561 tccaatgatg agatggtcgc catcttcaaa cgcatcattc acgcctgccg ggagctgcaa
     7621 ttcctggctt aaaactccta cgttctagcg aggtagcact gtggtcatcg tcatgaaaga
     7681 cagcgccagc gaggggatgg tgcaggctgt ctgcgagaaa cttgaggccc acggatttca
     7741 agtgcacaag gtggtcgggg tcaaccagac catcctcggg ggcatcggcg acatccgcgg
     7801 ggttgatctc cgcgaattcg aggtcatgga gggggtttac gaggtcctgc gcatcaccga
     7861 gccctacaag ctggcgagcc gggccttcca tccggagaac accgtggtcg aggtgggcga
     7921 ggcctccttc ggcggtgaaa tggtgacggt ttgcgccggt ccctgctcgg tcgagagcga
     7981 ggagcagctc tttaccatcg cccgcgaggt caaaaagtgc ggggccaagg tgctgcgcgc
     8041 cggtgccttc aagccgcgca cctcacccta cgcctttcag gggctgggag aaaaagggct
     8101 gcagctcatg cagcaggtcg gcgccgaact ctcgatcgca acggtatcgg aggtcatgga
     8161 gatcggccag ctcgatctga tgctcaggta catcgacatc atccaggtcg gcgcccgcaa
     8221 tatgcagaac tttgccctgc tgcgcgaact cggccacatc cgcaagccgg ttttactcaa
     8281 gcgcgggctc tcggccacga tcgaggagct gcttatggcg gccgagtata tcatgtccgg
     8341 cggcaattac aacgtcattc tctgcgagcg cggcatccgc accttcgaga cctatacccg
     8401 caacaccatg gatatctcgg ccatcccggt cacccagaag cacagccatc tgcccatcat
     8461 cgccgatccc agccatggca ccggcaggcg ggacaaggtg gcgccgatgg cgcgcgcggc
     8521 cgtcgccgct ggcgccaacg gcctcatcat cgaggtccat cacgacccgg atcatgccct
     8581 ctcggacggc gcacaatcga tctaccccca gcagttcatg cgcctgatgg aagagttgag
     8641 aatgatcgcc tgggcgatcg gcaaacgcat ctaaccggac agggaaacgg ccatggaaga
     8701 aaaactggca ttcaaagaga tcgccattgt cggcgtggga ttgatcggcg gatccctcgg
     8761 cctcgccctc aaacgcaagg gataccaggg ggtgctgacc ggagtggaca accaggaggt
     8821 cctcgccctg gcccggcagc ggggcgccat cgacaagggc tttgagaccg ccgatatcgc
     8881 cgacgccctc gccacggccg acctggtttt tctctgcacc ccggtcgcca atattctcca
     8941 ccatttacag actatcggct cctgcgtacg ccccggcacc ctgatcaccg acgtcggcag
     9001 caccaagcga cggatcgttg aaactgccaa catccatctc ccctcgcagt gcgatttcat
     9061 cggcggccat cccatggccg gctcggaatt caagggcatg gaggcggccg acgcctttct
     9121 tttcgaaaac accacctggg tgctcacccc cgccaagccg atgcgggagg agcggcgcaa
     9181 ggccatcggc gagctggtcg agatgaccgg cgccctggtg ctgctgatct cgccccatct
     9241 gcacgacgag atcgctgccg cggtcagcca tctgccccag atggctgcca tcgccctgat
     9301 gagcctggca gcccaacgtc agagtgagag cagccacttt ctcaagatgg ccgccggggg
     9361 atttcgggat atgacccgga tcgcctccag ccccttcagc gtctgggagg atattattac
     9421 cacgaatgcc gatatgatag gcagttatat cgacgcctat atcgaggaac tcaagcgggt
     9481 caagaccgca ttggataaaa aggagatgcg cggcatcttt gaccagtctg cccgcaaccg
     9541 cctttccatc ccgagggata cacgcggatt tttccgcccc catttcgaca tctcggttgt
     9601 cgtgcaggac cgcccaggca tgctcgccac catcgccggc gccctgctcg aaaagaatat
     9661 caacatcaag gatatcgaaa tcctcaagat ccgggagaac gaaggcggca ccatgcgcct
     9721 ggcctttgcc tctgaagttg atcgcgaaat ggctctggca ctgctcaaat cacgcggact
     9781 ggaatgcaga aagagggaat aacatgagcg atattctcgt aaaaccggct cgcacagtgc
     9841 aggggacttt ttctgtcccc ggcgacaagt cgatctcgca ccgagccctg atcctctccg
     9901 ctatggcgga agggaagagc aaaatcaccg ggctctccga tgcccgtgat gtggccagca
     9961 cccgccgctg cctcgaaaac ctcggggtaa aaatctcctc cctcaagggg ggtatcgtcg
    10021 ccgttgccgg caaagggaaa tacggctttc gcgcggccaa aggcccgctc gatgccggaa
    10081 attccgggag caccatccgt cttctcagcg gtgtggtcgc tgcgcagccc ttcaccacta
    10141 cgatcacggg ggatgagtct ctctgccgtc gtccgatgcg gcgcatcatc gaaccgctcg
    10201 aaaggatggg ggcccacatc gagagcagcg cctacaaggc acccctggtc atccgcggcg
    10261 gggccctgcg ctcgatcgat tatgcctcga ccatcgccag cgcccaggtc aagtcgtgca
    10321 tccttttggc cggcctctac gccaacggca ccacccgcgt caccgagccg agcccctcgc
    10381 gtgaccacac cgagcgcatg ctcgaggcct tcggggtcaa ggcccacttc agccagtcgg
    10441 gcgccggcgt ccagggaccc gccaccctgc aggcctgcga catcgacgtc ccgggcgatc
    10501 tctcctcggc ctcctttttt ctggttgcgg cctcgctgct tgccgattcg gagatcacca
    10561 tcgaacaggt gagcgtcaat ccgacccgca tggggatcta tgatgccctc tcggcgatgg
    10621 gcgcgatgat ccagacctcc cccgcggtca ccttccaggg cgagccgcgc gccgatctga
    10681 gcgtgcgcag cgccaggctc cattcggcgg ccctgggcgg ctccatgata ccgcgcatta
    10741 tcgacgaaat tcccgtcctc gccgttgcag ccacccaggc ccagggcacc accgtcatcc
    10801 gcgatgccgg cgaactgcgc ctcaaggaat ccgaccgcct caccgccatc tgcaggaatc
    10861 tccaggcgat gggggccaat gtccgcgaga ccaaggacgg catgatcatc accggaccga
    10921 cctccctgca cggggctgaa atcgagagct atgacgacca ccgcatcgcc atggcctttg
    10981 ccatcgccgg actgatcgcc accggcgaga ccctgattaa aaacggctcc tgcaccgaga
    11041 tctcctttcc cggctttttc gatctcctcg accggatccg tcatgattag cacgcgcacc
    11101 cggctgttcg ggctgatcgg cgacccggtc tcccacagcc tttcgcccat tatgcagaac
    11161 caccttttca agctcttcgg actggacggt gtctatctcg ccttcaggac agcgggggag
    11221 aggctggccg aggccatcag cggcgcccga gccctcgcac tggccggcct caatgtcacc
    11281 atcccccaca agagtgctgt tgtgccgctc tgtgattacc gctcgccgga ggtggagctg
    11341 ctcggcgtcg ccaataccct ggtcttccgc gacggcagga tccacgcctt caccaccgac
    11401 cacagcggct ttgtcgactc tctcgggggg gagaagagcc gcttcagtgg cagtcgggtc
    11461 cttctcttcg gcgccggagg ctctgcgcgt gccgtcgcct ttgcgctcgc ccggctgggc
    11521 atcgcccgcc tcaccatcgt caaccgctcc cggaccaggg gcgaggaact ggcagagttt
    11581 tgcagcagag agctggccat ctccgcagtg gaggccattt ctccccttga tccggtgctt
    11641 ggaggaaaga tcaccgaggc cgccatcctg atcaatgcca ccgcctgcgg catgttcccg
    11701 cacatcgaag agtcgcccct gagcgccttt gacttgattg accggcgcca tttcatctac
    11761 gacctggtct acaacccccg cgagacccgc ttcatgcgcg agtccggggc gcgcggcgca
    11821 gcggtgcagg gtggactgga tatgctcatc tttcagggac tggccgcctt gaaccactgg
    11881 ttctacagcg attaccggct tgatccggcc gaaatgaaaa aactgaaatc tctactcaac
    11941 cgcgagctgg catgatggaa aaacttcgct ttctcaccgc cggtgaatcc cacggccagg
    12001 gcctggtcgc catcctcgag gggattcccg caggactgag gctcagcgcc gccgatattg
    12061 cccccgatct caaacggcgc cagcgcggct acgggcgcgg cggacgcatg cagatcgaaa
    12121 aggacaccgt gcgcattctc tccggtgtgc gctacggcaa gaccctcggc agcccgatcg
    12181 ccatgttcat ggagaatctc gactggccga actggacggc gaagatggcc ctcgaggaac
    12241 ccgtcgagcc cgtcgccgcc ctgcacatgc cgagacctgg ccacgccgac ctggccggca
    12301 tgatcaagta ccgtcaggag gatctgcgca acattctcga acgctcctca gcgcgcgaga
    12361 ccaccgcacg cgtcgccgcc ggtgcggtcg cgcgcaggct gctcgccgcc tttgggatcg
    12421 aggtctacag tcatgtcctc tccatccgcg gcatccaggg gggcatcact gccgccggct
    12481 ttttcaaggg gcggacatca cgggaccccg aactgctggc ctcctggcgg gccctgatgg
    12541 agagggtcga atcctcaccc ctggcctgcg ctgacgcgga ggcggaaacg gagatgatcg
    12601 ccgccatcga tggcgcccgc gaaaaaggcg ataccctcgg tggtgtaatc gaaattgccg
    12661 ccctcggcct ctttccgggc ctgggcagcc acgtccactg ggaccggcgg ctcgatggtt
    12721 tgctcgccgg agccctcatg agtattcctg ccatcaaggg cgtggaggtc gggctcggcc
    12781 ttggcgtcac cctcaagccg ggttcgcaga tccatgatga aatctttcac gaatccggcc
    12841 agggttttta tcgcggcggc aacaatgccg gcggcatcga ggggggaatg agcaacgggg
    12901 aacccctcat cctgcgcgtc cacatgaagc ccctgccgac cctggccagg cccctgcgtt
    12961 ccgtcgacgt tgccaccctg cagccgcaat cggcccacca cgaacgcgct gatgtctgcg
    13021 ccgtccccgc cgcagccgtc gtcgcggagg ccatgaccgc cctcgtcctc gccgacgcct
    13081 tgtgcgtgaa actgggcggg gactcgatca gtgaaatgca gcagcattac gcgagcctgg
    13141 cgcatgtacc cctgggatgg taaaaatata tattttatgg gttttatggg caccggcaag
    13201 agcaaggtgg gccgggcttt tgcccgcctt ctcggcaggc ccttcgccga ttccgacgac
    13261 ctgatcgtcg ccagggccgg catgccggtt gagcagatat tcaagcagag gggcgaggag
    13321 gaattccgcg ttgtcgagga cgagatcgtc gacgaaatca gccggcgctc ccagtgggtg
    13381 atctcgctcg gcgggggcgc tgtcctgcgc caggccaatt gggagaaact tcagacctcg
    13441 ggcctgacca tctgcctcga ggcctcgctc gaggtgctgg ccagccgcct cgcccgtaaa
    13501 aacgaccgcc ccctggtgcg agggctgagc ggcgaggctc tgcacaaccg cattcaggaa
    13561 ctctacagcg cccgcgctca ggtctacgcc cgcgcgaact atcattttca gagccgtgag
    13621 gaggtctcgg ctgcggaact ggcggaggag atctttctct acctgcggga cgagccatga
    13681 ggagcgtgcg ggttgaactg cagcggatgg tctcgcccat ccatatcgga ttcggtctcc
    13741 tggagaggat gccggagctg ctggcgaggc ataacctcca gggcgagctg gttatcatct
    13801 ctgatgagcg cgtcgccggg ctctacggcg aaaggctgct gaccgcgctc ggagcggccg
    13861 gcaggtccgc ccatttcctg cgcgtgccgg agggggaggg ggccaaatcc ctcgaacagt
    13921 gcgaggtgct ctggcgcaag ctcatctccg gcggcttttc ccgttcaacc atcatcctcg
    13981 ccctcggggg cggagtggtg ggcgatctcg ccggcttcgt cgcttccacc tttttgcgcg
    14041 gcgtccgcct cgtgcagata cccactaccc tgctcgccca ggtggacagc agcgtaggcg
    14101 gcaaaaccgg tatcaaccac cccctcggca aaaacctcat cggcgctttt catcagcccg
    14161 aactggtctg catcgatccg gccctgctcg ccaccctcga acgccgcgac cgcctcgccg
    14221 gattggggga ggtggccaaa tacgccctca ttcgcgatcg cgccctcttc accctgctcg
    14281 cgctcaacct ggaggccctc accgagctcg cctatccgga gctttgcgag tcgatcatcg
    14341 aaaccagctg caccatcaag gctgaaatcg tctccgccga tgagcgggag aggggagaac
    14401 gggtcctgct caacttcggg cacaccatcg gccacgccct ggaggccgca ggcggtttca
    14461 gccacttccg tcacggcgag gcggtaatcc gcggcatggc cggtgcgatc tggctctcgc
    14521 ggcggatcgg acgcctctcc cgtgccgaag cacagaccgc tcttgatctc ctcacccgtc
    14581 ttgcgcctcc ggcccccccg ccaggcctcc ggccagcgga tctgctggcc tggatggccc
    14641 gcgacaagaa acgcggcccg gaaggacaga cctggatcct gctggaggga gtgggcaagg
    14701 ccttcatcac ccgggaggtc gcggaggatt tgctgccgcc ggccctcgcc ttcgcctgcg
    14761 ccggtgagtg gcgtgctccc tgatcgtcgg gagaccctta taaaccgcca cattccgccg
    14821 ccccctcact gcctcaggag ttgacagcat gttcaccgtt ctcgtgattc acggtcccaa
    14881 tctcaacctg ctcggcgagc gcgaacccga acagtacggc cgccgtaccc tggctgaact
    14941 caatcgggag ctggaagagg aggcggcaaa actcggcgtg cagctggaaa tcctgcagtc
    15001 caatcatgag ggtgtcctca tcgattttat tcatgcgcag cgccgcaacg ccgccggcat
    15061 tctcatcaat cccggcgccc tcacccatta cagctatgcc ctgcgcgacg ccatcgccgg
    15121 ggtcaacctc cccgctgtcg aggtgcatct ctccgatatt caccagcgcg aatccttccg
    15181 ccgcatcagc gtcatcaggg aggtctgcct ggcgcagatt tccggcaggg gctttgattc
    15241 ttatctgcaa ggattgcact tattggttga ttatatccgt aaaaatggtt aaaatagagc
    15301 gcgaattggc cggttatacc acttgccagc ccgctttgct tgtatcaaaa cgattcatca
    15361 ggatcaattt gcatcacttt ggagcctcca accggccttt aatagccggc aggaatgttg
    15421 aatgcatttc cggtgttata actgcaaagt aatcaatgcc tgcgcctgaa aaaacaccat
    15481 ccctggcgct ggtattggca cgattcatgc agtcctcatt cgtcaaatta tgcataaaca
    15541 ggagggcaac cagatgagcc ttcggctaca gaatcagcat catgcatttt tcattttgtt
    15601 tgtactcgcg ggaatcccgg gactgctggc cggcgccggc atcgaacggc ccttcgaccc
    15661 ggttgtggtc acgggcgctc agctcggcga tttcctcggc gccgatacca gcgatatcta
    15721 tgtctacgcc tgggtcgagg gaagctggcg gcagatcccc tttcagatcg accaggtgga
    15781 ctggaacagc gaactggccg agtacagcta cttcgcccca catgatgccg tgctcgattc
    15841 cctggatgag ctttgcttca tggcggtgga tatgggagac agtgtggctg acaaccgctg
    15901 gataggagac atcggttcaa ccctgtatca gcgctaccag gtcgccgcct gggacaccag
    15961 cgtggttccc gcccgtaaat catacgccta tgtctatcgc tcctcctcca tcactccggc
    16021 gttcagctcc tatatgactt atgaaaccgg cgtcgtcggg gtttcggata ccgtcagtgc
    16081 ccagtcctac atttatggcc agaacgcgga tgccattccc gattttctca gcctcctgac
    16141 cgacgaggga cgcggccccg atatcctcga ccgctggaaa atccgcttca agggcagcat
    16201 cggcccttat tggggcacgc ccgagtacat cgagagcgag gagagtgccc tgcgcgatcc
    16261 tgccctccag gtccgcgccg gcgcggttcg cgtcatccac agacgcgagt tcggcatcaa
    16321 catgcagggc aatgagctgc cgctctatct ggacctggaa tcggtctttt atccctggtc
    16381 ctacaaaatc aatctggcca aggagggatt gccgggctac ctcggcatgc aggagatgcg
    16441 gcaaaccgtc gattacctgc ccaacattac cggttcgctc ttccactggt ccggcagcac
    16501 cggcatcccc gtcgatggag ccctcgatat catccccgac aagagttttg atacccctgc
    16561 cgtcaactgg tatatggtac agggcgagtt cggcatggcc gctaccgttt tcaatatgct
    16621 gcccatcacc ggcacccagc tctcgctcta ctacattgat gactcctcca ccgagcatgg
    16681 ggccgagcgc gacggcatcg gcgataccgg cgatatgatg gcctatgccg aaagtggcgt
    16741 ccagctcacg gcgtcgggct cggatccgat cgatatcgag gacgacgatg aaaatctcgc
    16801 tgccactttt tactatctgc cgggctacca tacggcggcc tatggcgatt cccttgccga
    16861 ttacacccgc catcccctcc ggatcgtcgt cactccgcgc accaacactg tcattccggt
    16921 ggagatgact gctctcaagg cgcagctcgt caagggaagc gtccgcctcg agtggaccac
    16981 ggtctcggag tccaataact acggctttga cgtcgaacgc cgcggtgcgg ccgagagcag
    17041 ctggagcaag atcggcttcg tcaagggcaa cggcaccagc cagatcgccc acgcctacca
    17101 gtttatggac aaggagcccg cgacgggcag caacagctac cgcctcaaac agatcgattt
    17161 tgacggccgc tccacctact cccaggaggt cgctgttgac atccagctgc ctgaacggct
    17221 tgaactgggt cagaattatc ccaatccttt caatcccggc accggcatca ccttccagct
    17281 tccggccgga gagcagcagc gcgtcactct ggctgtctat aacctgctcg gccagaagat
    17341 cagaaccctg gtcgactcca atctggagcc cggcacccat caggtctact gggatggccg
    17401 cgatgaccgc gggctgaatg ccgtctccgg cgcctatctt taccgcctcc agcttggcgc
    17461 gcaggttctt acccgaaaga tgatcaaaat gcagtaactc ccgccatctg aggcggaata
    17521 gagggtataa aaaaacgccg tcccccgcag ggagacggcg tttttatttt tatgcccgcc
    17581 cgctctattc ggccggcggc agagcagcgt ccgggatctg cgccgaactc ttctgccgcc
    17641 tgatctgacg cttgaactcg gtgctgtaga tgtccagcag ggtgatgaag agcgaagcga
    17701 ccaccgggcc gatgatgaag cccatgatgc cgaaaaagcc gatgcccccg agggtggaaa
    17761 agaagaccat gaggtcgtgc atgcccgcgt cgcggccgac cagccgcggc cgcagcagat
    17821 tgtccacatt ggagatcacc agcacgctga taagcagaat ggcaatcccg gcggccgctt
    17881 cccccatggc gatcttgtag atggcggctg ggatgaggac catgtaagcc ccgaccatgg
    17941 gcagcaggga gaggatcacc atcaccatgc cccagaggac gatggccttg aaaccaaaga
    18001 tccacatcgt cagagccccc agagtgccct ggatgagccc cagtatggcg ctccccttga
    18061 tggtcgcgcg ggaaatcgcg ctgaagcggg agacgatctt ctgcttgtag tcatcctcga
    18121 gggggaggaa acccatcacc tgggcgatga tccggtctcc atccttgaaa aaatagaaca
    18181 tagtgaagag gatcatgatc agattggtga ggaaggcaaa ggtgccgcgc gaggtggcgt
    18241 tgatcacctt gcccgcatag gtggagatgg ttgtaatccc ctccttgatg gtcgcctgcc
    18301 agtcgatctg atcgagcttg aatttggcca ggatgggatg gctggtcagg ctttcgaggt
    18361 tgaaatcccc gctgttgtag aactcttcca gcgccgggcc gatcgagtcg tagaggttga
    18421 tggcctggtt gataacaatg ttgaccagga cgtaaagggg aatgagcagg accagcagca
    18481 gtaccaggca ggtgagcagg gagctgagtg cggtccggcc tttggtccag cgcacaaacc
    18541 gtttgaagag ggggtcgaac agggtggcaa acacagccgc cacaatgatg gggaccacga
    18601 atatcttgat catgaataca aacaggacca gtacagccag cagggaaaag agcaggaaat
    18661 actggttgaa atccatgcgg aagagagtct ctttttctcc tcccttagcc ggccctgcgg
    18721 ccgggttctt ctggatatgc tccatgacga ttctccgggt gcttgacagt tttcgggatg
    18781 ctgccccctg atgcggctgg cggaatctgc ccgcagcagg accatgctcc ctcatgaact
    18841 aacgcagtat ttaaacattt tttggctcca aaacaagcac tatttgacgg ctgctgaggg
    18901 atggcgcagg gggccgggca gcgcacgagc ggttaatatt ttctttgctt cttgtccatg
    18961 gatttgtaaa ttgtcttcgg ttgcgacaaa tcctcttttc cctttcacat tgcccggagg
    19021 ctcgatgaag atccacgaat atcaggccaa ggaactgctg gcgcgctacg ggattcccgt
    19081 gcagcggggt gcggtcgcct tcaaacccag ggaggtgcgc cggctcgccg aaaagatggc
    19141 cttgaaggag atcgtgctca aggcacaggt ccatgcgggc ggacgtggca aggcaggcgg
    19201 catcaagctg ctccattcgc cggcggaggc cgaagatgcg gccgccaaaa tgttcggcat
    19261 gaggctggtg accccgcaga ccggcgccga gggcaagatt gtacgcagaa tcctggtcac
    19321 cgaggccgcg gaaatcagcc gcgagctcta cgcaggcatc gtgctcgacc gagccaggcg
    19381 ccagctggtc ttcatggtca gccaggctgg cggggttgag atcgaaaagg tggccgagga
    19441 gagtccggaa aagatcatca aggagtacgt cgaccgcgcc gccggactgc agcccttcca
    19501 ggcccggcgc atggctttcg ctctcggcct gaccggtccc cttttccgcg agggggtccg
    19561 cctccttctc gccctctatg aggcttgcat ccagtgcgac gccagcctgc tcgagatcaa
    19621 tcccctcgtg gtcacgcccg accagaagct tcttgctctg gacgtcaagc tcaatgtcga
    19681 cgacaatgcc ctcttccgcc acccggatat ccaggagatg cgagacaccg gtgaggagga
    19741 tccccttgag gtcgaggcct ccagctcgaa tctcaactat atcaaactcg acggcaatgt
    19801 gggctgcatg gtcaacggcg ccggcctggc catggcgacc atggacatta tcaagctggc
    19861 aggggcggag ccggctaatt ttctcgatgt tggcggcgga gcctccgccg agaccgtcga
    19921 gaacggattc cgcatcattc tctccgaccg caacgtccgc gccgtgctca tcaacatctt
    19981 cggcggcatc gtccgctgcg accgggtcgc ccgcggcgtc atcgaagcct cgaaaaagat
    20041 cggtgttacc atcccggtcg tcgtccgcct cgccggcacc aacgccgaag aggcggcgct
    20101 cctcctgcag cagtcgggga tgaatttcgg cgtcgccagg gacctggccc aggccgcgga
    20161 gctggtcgtc gccgcattga acaagccaaa ttgagaacgg gagcccccta tgagcatact
    20221 ggttgacaag aaaacgcgcg tggtcgtgca gggcatcacc ggcggcgaag gctcgttcca
    20281 tgctgcacag atgatcgaat acggcacgca ggtagtggcc ggcgtcaccc cgggcaaggg
    20341 cggacagctc ttcgagaagc gggtgcccat ctatgatacc ctcgccgagg cggtgcgcga
    20401 gcaaaaggcc gacgcctcga tcgtcttcgt ccccgcccca ttcgccgcgg acgcggtcat
    20461 ggaggctgcc gacgccgggg ttaaactggt cgtctgcatc actgagggca ttccggtcgt
    20521 cgacatgctc cgcgtcaccg agtatctggc caaaaggccg acccgcctga tcggccccaa
    20581 ctgcccgggc attatcaccc ccggtggctg caaactcggc atcatgcccg gcttcatcca
    20641 caagcccggc agggtggggg tggtctcgcg cagcggcacc ctcacctacg aggcggtcgg
    20701 tcagctcacc aacctcgggc tgggccagtc cacctgcatc ggtatcggcg gcgaccccgt
    20761 catcggcacc acctttctgg acgccctgca gctcttcgag gcggataaaa agaccgaagc
    20821 ggtggtgatg atcggcgaga ttggcggcac cgccgaggag gaggcggctc gctatatcga
    20881 ggaaaagatg accaaaccgg tggtgagctt catctccgga cagaccgcgc ccccggggag
    20941 gcgcatgggc catgccgggg cgatcatcgc cggaggcaag ggcacggctg cggaaaaaat
    21001 ggaggccctt gcggaggccg gtgcggcggt ctgctccagc ccggccgata tcggccgcat
    21061 ggtgaaaaag gtcctcaccg cacgccggaa tatggcataa aaaaggggtg tggaaagaaa
    21121 aaaaggaacc tttggcggga tggcgccgtt agataaataa cagcactacc tttccattct
    21181 tcaccctcct tgcaataagg gcctgtgttt cagccggaac caggcccttg ttgtttacag
    21241 gaggtaataa attgcgaaaa aatggaggat gctgccggcg atgacgaaga gatgccagat
    21301 cgagtgggcg tagggctgtt tcttccagac ataaaagagg atacccaccg tgtaggcaag
    21361 ccccccatag aggagaaacc gggtgctgag gggcggcatc cgcgtgatga tctggttgta
    21421 ggcgatcaga aaggaccagc ccatcacggc gtacatcacg gttgagagta cgcggaagcg
    21481 gc
//
LOCUS       DAOPWU010000022        19187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_2902_length_19187_cov_4.613945, whole genome shotgun sequence.
ACCESSION   DAOPWU010000022 DAOPWU010000000
VERSION     DAOPWU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2902_length_19187_cov_4.613945"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PKM23_02550"
     CDS             <1..1048
                     /locus_tag="PKM23_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90382.1"
                     /translation="FPAFLFIVGLSLPIALEKRLGRGESKIRIAGHILTRTLSLLLMG
                     VLMVNTESIASKGHIDPRLWQLLFYGSAVMIWLASGAERRRIVWARRIIGIVILAALA
                     FAYRSEGGAGLRPHWWGILGLIGWSYLVTALLYLVIRRRPAGFAAAIILLYLLYFADR
                     TGQLAFLGPLSSWIGIASVLGSQPAITASGTLLSILLFSTDQPLAVRLRIIFLFALIL
                     GTIAVLLHSLSNLSPLFIYNKNAATPPWCLISSAWTALLFALIYWIVDGRGLTTGTRM
                     LATAGQNALFAFILGPIFYLLIGMLPVMADGRSLYGMLGAGFATGFWRSLIFALAGTW
                     LTAAMQRSGRYLRI"
     gene            1050..2123
                     /locus_tag="PKM23_02555"
     CDS             1050..2123
                     /locus_tag="PKM23_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNY90383.1"
                     /translation="MKKRPGIALLLTLLLATALPAASGKKAEPWCALHLLGFTSDSSL
                     IRLESQLPALAEAGVNMLILEVDYNFDYQSHPELCGTGQPITRAGAKWLTKACRRQNI
                     ELVPQFQCFGHQSWAGTTYALLTRYPEFDLTPGAYPGNQGIYCREWDPLNPAVHAMIF
                     DLLDELIKAFDADMIHVGMDEVFLIGDSLSPSTLGKNPASVFAGAVNDLYEHIVKKRD
                     KGMMMWGDRLIDGEKYNLGEWEASKNGTAAAIDSIPKDIIICDWHYEPREEYPSLPLF
                     IDKGFRVLPTSWKKIEAVRAFINYSQALNHPDMLGHLFTIWSSKQDSLALYPPLAQFG
                     RQVRAGAMPAAHSPGAVNAPALR"
     gene            complement(2101..4722)
                     /locus_tag="PKM23_02560"
     CDS             complement(2101..4722)
                     /locus_tag="PKM23_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNY90384.1"
                     /translation="MRKLLLLVTIILGAACAPSGESLIPAGELRRAVHAQLPIQKELS
                     RPRQQELFGVFSQKLTAAERSALEWLYAWMPLSDLADYDGEFYLRQVHTTLQVREEMA
                     WGRTLPADLFLHFVLPLRVNNENLDSARVVLSGLLRERVRGMTMRQAALEVNHWCHEH
                     VTYRGSDMRTSAPLATMRSAIGRCGEESVFTVAALRAVGIPARQVYTPRWAHSDDNHA
                     WVELWADGEWYYLGACEPEPDLNMGWFTEPARRAMLVHTRVQGPWAGGEEVLATSPRH
                     REINLLERYAMTRPLVVRTLDGADRPLAGANVSFRLYNYAEFYPLARRTSDAEGFCTF
                     CTGRGDLLIWAHRDSLIGFSRAAGADTVTVILRPRAPREEIFELDFNPPLARPPLPDT
                     IPAEARGRHARRLAREDSLRRACESTFMDSLSSARLAAESGLEPDPVWRIIRSSRGNH
                     EEIARFISAGATRNRSLVLPLLQMVTEKDLRDARAEILLDHLPSHIPGTKLPDGIYEQ
                     YLLNPRVRNEALTPWRRLLQEGMSRETGQSVVASPGMLAAWVEKNIRLEADANYYGVP
                     LTPRGVWELRAADALSRDIFLVALCRSMNIPARLDPAFERPQYYDLSHQGWRDFGEDP
                     AAIQAGSRLTLANREATISRPEYWRHFTLARLEGGEYKTLELEGIDFSEGRGMLELPA
                     GNYLLVTGRRLQSGAVLARLHFFDLAPGGQRSIDLVWRRSGEPLKPLARLPGGLMLTD
                     RRGRRLDLAELARPQGLILAWLDPGREPSQHILREWHELKQLYEAWGGRIAAVMAAGG
                     EGRLGAYSLPRQLLVVQDEHDGVRRRLGEALAGELSVFPVLVYVNPAGEILWMNQGYQ
                     VGLGDRLLQVITAAQER"
     gene            complement(4800..7910)
                     /locus_tag="PKM23_02565"
     CDS             complement(4800..7910)
                     /locus_tag="PKM23_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNY90385.1"
                     /translation="MIEREIQLYETRIGQFVDRLPSYFYSEQLPLEAEAALADPPLSF
                     ARRLELAYRPIAVGEKWGADWQTAWFHLTGTVPAAWQGRPVVARINLGGEGLVFAADG
                     TPTHALSWHSLWKPEFRRDRVPIADPARGGEQVDLWIEASAAQIFGLRLVADPLQEDP
                     RRHGHLEAVVQDLTLAVFRPSVWHLWLDARLLFNQMKALPEKSVRRARLLRALMQAAD
                     RFQGDDGSTAAARALLAVELVKKSGPSDLVAHAVGHAHIDTGWLWPVSETVRKCARSF
                     AAQIDLIERYPGYVFGASQAQHYAFVKERYPALYEKIRAKVAEGSWEIQGGMWVEADC
                     NLIGGESMVRQILHGVRFFEQEFGITVRNLWLPDVFGYSAAMPQILQKSGLGYMVTQK
                     ISWNQFNRFPHLFFFFKQKTAYEIIVHFPPEDTYNSEFWPSGMRHAQENFAERDRLDE
                     FLVLFGIGDGGGGATEEIIESGLRQNDLEGGPRVRFGKAQEMLDRLGARRELLQRWNG
                     ELYLELHRGTLTTQARTKFMNRWCEHKLREVEALFSALPAAEYPAAELDRLWKTVMRN
                     QFHDILPGSSIKLVYETTRREHDEVRKELTSLAGRAGAGLLHEQEESLTLVNTLSTPF
                     TRPTFLPAAWIGHVILDEADQTVPTQVREGNLVALAEVPALSSVVLRRGQTLPEVDLP
                     PEPRVSEPLAPASLEGVVLENDLILYELAGDGTIARIFDKERSCEVMLPGQSGNRISL
                     YEDRPANWDAWDVDIYYENQLLQQAGLTGWRRLGSGPVAQMMLLQFRVGDSFIEQRLC
                     LVRNTKRLDFVTHVHWRERHRMLRVAFDADVTASEASYEIQYGTVRRPTHRNTSWDMA
                     RFETVGHRFVDLSEPTHGVALLNNGKYGHKVLDRTIDLNLLRAPTSPDPDADRGEHDF
                     VYSLLPHAGALEESAVYAEAAQLNQPLALFEDRSGRVDFPCRLENEAAILSVLKKAEG
                     DDAIILRLYEPRGRRASTRLLLTDPGRRVFATDLLEREIGELPQREGAVALDFGPFEI
                     KTLKLV"
     gene            complement(7914..9443)
                     /locus_tag="PKM23_02570"
     CDS             complement(7914..9443)
                     /locus_tag="PKM23_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNY90386.1"
                     /translation="MNSSWMEKKMIKKYLGFAFLLTSLLIGCAKKDEGWLVKVKDQTL
                     RDDILTRRYKMSREFSQHPAITPEILKVFIEKTLLNNLFFQAEGNALKLDQDSTIAEQ
                     IRQEERRMLTRNNGPLFKAVVPASFSVSEEEIRNAYEWGKQEFKLSHILVKSPTLADS
                     IYSALMAGADFKTLVTRYSMDMNTIENGGSIAPWLSWNQLAPPSAEAVRNTPTGSYTK
                     PILGGFGYQILRIDERRERQQPPFEQARTEIEGNLKNQKQGLFVENYTNDLYSKFNLA
                     IDSLLFVKIAPALQGNSAGAKVDYALISPEDQKKTLVSWKGGQWSVRETLDKYLQMTR
                     GNAYRLTRYEDVADFARKANLQELMLLDARARKLDQTEEFKRDFFYAKNQLLGQKCRE
                     KLITRAVKVSEEEITAFYESHKSEYKDRPLAELRSTLRSRIYSAKMAELQEKVTADLR
                     KKYPEKWNEKALKKTAEALNAEKAALSKAPAQPQARTGQPGGMPGQRPDMPPAPPQGV
                     R"
     gene            complement(9514..11751)
                     /locus_tag="PKM23_02575"
     CDS             complement(9514..11751)
                     /locus_tag="PKM23_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNY90387.1"
                     /translation="MKFAPLCCSFLLALTASAAAAVSLVPQPVLMKTGKGSFAFSAST
                     PIVYPAGSPEAGRIARLLGAEINQSSGLDLAILPSSQAGRSAPIRLLLDPALKEAGLE
                     GYRLTISRRELLLAAPSPAGLYRGGQTLRQMLPSGSGKRTLALPALEIIDQPRFSWRG
                     VHVDVGRHFHSVSYMKRIIDWMALYKMNRLHWHLTEDQGWRIEIKKYPLLTGIGAWRT
                     EPDGSRYGGFYTQEEIKEVVGYAADRFIDVVPEIEMPGHCMAALAAYPGLSCTGGPFA
                     VQTQWGVFKEVYCAGREETFAFLEDVLSEVIELFPFGYLHIGGDEVPRDRWQACPRCQ
                     ARIKAEGLDDEAELQSWFIRRIEQYLSAHGRRLIGWDEILDGGLAPGATVQSWRGMEG
                     ALIAARSGHDAIVSPGSHTYFNADISAIDLRKAYSFEPVPAGLEPERIPYIIGGECCL
                     WSERITEENLDYQLFPRLLALSEVLWSHPGQRDYADFLRRVRNHYPLLETLGIGFGPE
                     ARPVSLLSRFHPDEKFHTVTLESGEEGMALHYTLDGAEPTTASPRYTSQLTIDRPTRL
                     RARAFRDGRGYGESAGADFDFHLAFAAPLQIDHSFSPNYPAGGGRALVDGLRGSIAYG
                     DGRWQGYEEVDLIATIDLGEIRPLHRLRAGFLQDTGSWIFLPAYVEWSLSRDGSSFRT
                     VAAFDHPTPAGTSEQRIQEDQTELPGTMARFVRLRARNVGLCPAWHPGAGGKSWIFVD
                     ELVVQ"
     gene            complement(11768..14266)
                     /locus_tag="PKM23_02580"
     CDS             complement(11768..14266)
                     /locus_tag="PKM23_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNY90388.1"
                     /translation="MKRLLVILLLALQPAALLAREGEPLQLSHGLACRLPEHQRRAMI
                     PADPVEALVTAGKKVAPMAGDLLVQGEEGESWKAVTADSAGWFSDPTLRNGWVHFRIT
                     RLQPALFLLEGMGNSMVYVNGTPRIGNRYRAKDQFESWEPRFDYGLVPIQLDEGENHL
                     LFRCSRGRLKVVLHPLTKTALFNPLDLTLPDLRAGEALESPGGIVVLNGTNAPLSGLF
                     IRGRGEGVKVPETPLPELLPLGLRKVAFPISAAPAGAAGELPLTLTLLRRSGAGEEIL
                     DETVILLRRVEAGATHRITFISGIDGSVQYYAVNPASGPADAPAALFLSLHGANVEGA
                     NQAASYAPKSWGHIVAPSNRRPYGHNWEDWGRMDALEVLDLARRRLKIDTTRIYLTGH
                     SMGGHGTWHLGATFPDYFAAIGPSAGWISFWSYGVRDTSSTGDPALQLLRRASTPSNT
                     FAIAGNYSHQGVYIIHGSKDDNVRVDQSRRMVEQLETFHHDWYFHEQPEAGHWWDLSD
                     APGADCVDWMPLFDFFARHARPGAERMRRIDFTTANPGISARSGWLTILDQEIPLAVS
                     RARIEFYPAARHFQGSTGNIARLALRTPAGAGRAVFHVELDSQRIDLEGAETLWLEKR
                     AGRWSAGPALRADEKGPHRFGPFKEAFNQRMIFVYGTRGSATENRWALQKARFDAESF
                     WYQGNGSVDVVADVDFNPDADRDRSVVLYGNSATNAAWKNLLAESPIQVRRSIVQIGK
                     QILRGDDLAALFIQPRPGSDKACVAVIAGTAPAGMRLTQNLPYLYAGAALPDFTVLSS
                     AILRENSGGVAAAGFFDRHWRLDEANMVLPSPKK"
     gene            complement(14367..16745)
                     /locus_tag="PKM23_02585"
     CDS             complement(14367..16745)
                     /locus_tag="PKM23_02585"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HNY90389.1"
                     /translation="MKYGDFSPDGKEYIIDNVATPSPWINYIYNGHYFATISNNGGGI
                     SYYRSPLHGRVTRYRINDVPPDRPGKYIYVKDRDSGEIWSLTWQPVGRNLEAYRVRHG
                     FGYTRVEARVEGIRSDLLYFVPPDDHLEIWQALLRNESGRPRRLSVYGYVEFALGHAM
                     IDLINQCDDQHFNRVHFDPSVRSLFATKTYWVTGTRGTQHQENKEWDQWAFFSVNQPV
                     TAFETLRERFIGNYRNENNPAAIGQERLSSQETDFGNAIGALQCDIELAPGQELPVVF
                     NLGVVAKDECKQLCRVLPVRYHEPAARQRAWEKLCGKWERYFQVTRIQTPEPGADIFM
                     NYWTPYQARVAFEVGRVASFYYWGIGRGFGFRDTAQDTIAVTLSDPELARQRILLLSR
                     QMFLDGHVYHHFHGDGQGEVTGHCDDPLWYILAISDYIKQTGDWAILDNQQPFIDAPA
                     APLIEHLHAVVRFVRGNLGSHGLPNFGRGDWNDTLDYIGGDDGGESVWGAMFYVAMLN
                     HLIELLTHLGLAGPLATAVETRDLLKEAVQNHCWDGEWYIRAFGARERRIGSRTNTAG
                     RIFLNTQSWAVIAEIDASGRLEQAMESVWNHLNTPYGPKKCAPAFREIDPTIGLVTRC
                     VAGKKENGAIFAHPTAWVIMAECLLGHGDRAFTYYKEMLPNAIDSDIFVAEPYVFSQY
                     VTSDEHSAPGRASHSWQTGTAAWMYRVMMDYCLGVRATFDGLFVDPVIPSSWPGFTLE
                     RVFRSTRYVIRVTNPDGVQRGVRSILFDGQPISGRLLPLTDKKRVEVEVVMG"
     gene            complement(16742..17851)
                     /locus_tag="PKM23_02590"
     CDS             complement(16742..17851)
                     /locus_tag="PKM23_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNY90390.1"
                     /translation="MKKALISLLLCAAGALSALELPKVPFSPPQYICCRTTRPLIIDG
                     NLGETDWQRAAWSEAFVDIEGDQKPRPRFNTRVKMLWDDHYFYIAGQMEEPDVWASLR
                     EHDSIIFYDNDFEVFIDPDGDTHRYYELELNAFATHWDLYLDRPYRDGGNPTFFWDIR
                     GLQKGVVVQGSINQPGDADTGWTIELALPWAVLKEQAPEGRAPRPGAYWRVNFSRVEW
                     RTRVENGAYVKLTDPATGKSLPEDNWVWAPTGLINIHYPELWGFVQFSSHPAGEKGGE
                     FNVPEAEQIRWFLRQVYYRQRQLHRDTGQYAKNWSGLKLQLDRPAGCTWPPSLSAGPE
                     TWEALLTAADGKSAWHIRHDGLIWPTASQEERDRR"
     gene            complement(17875..>19187)
                     /locus_tag="PKM23_02595"
     CDS             complement(17875..>19187)
                     /locus_tag="PKM23_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY90391.1"
                     /translation="RSGWCPGDIVYPLRLDLSAALTPGPHTIRYAVENIRPADASGTG
                     YWRVSSYLSGAGDVASWRPSRIAISFESGKIHPVGTPGPVRIDLVDDSGLPVFVAAAE
                     IRISAPNPELLFSLDGIVWTNPLDCAITTGSAQIWMKSSSALQTEFTAADLNGLLASP
                     QPLTVTFYDFEVGPDEINLALTAAARADCECNAVSENAQKAIDGSLATKWCCNNGAPD
                     WLELTLPDTAAMNYFVIRHAGAGKAPAGDPGAGDGPGMNSADFTIQLLDSSGSWRNAL
                     SVRDNPADQSGSVSYHALPEKVESRQVRLYLTKPDVSRIYEFEIYYRASSPVRLEDVE
                     EGMQPERLQLVGAWPNPFNSSAAIRLFVPELMEVRASIMNSRGQMVAQVANGTLPRGL
                     HTFQWQGRTDAGQAAASGIYYINVLGVDAAGQRSLQVRPITLMR"
BASE COUNT         3434 a         5830 c         6433 g         3490 t
ORIGIN      
        1 ctttccagcc tttctcttca tcgtcggcct ttcgctgccc atcgccctgg aaaagcggct
       61 tgggcgcggc gagtccaaaa tccgcatcgc cggccacatc ctcacccgca ccctcagcct
      121 gcttttgatg ggggtgctga tggtgaacac cgagagcatc gccagcaagg gtcacatcga
      181 cccgcgtttg tggcagctcc tgttctacgg cagcgcggtc atgatctggc tggcctcggg
      241 ggccgaacgc cggcgcattg tctgggcgcg caggattatc ggcatcgtca tcctggccgc
      301 cctggccttc gcctaccgca gcgagggggg agccggactg cgaccgcact ggtggggcat
      361 cctcggcctg atcggctggt cctatctggt gaccgccctg ctctacctgg tgatccggcg
      421 tcggccggcc ggattcgccg ctgcgatcat cctgctttat ctgctctatt tcgccgaccg
      481 caccggccag cttgccttcc tcggccccct cagctcgtgg atcggcatcg cttcggtgct
      541 cggatcccag ccggccatca ccgcctccgg aaccctgctc agcatcctcc tcttcagcac
      601 ggaccaacct ctggcagtgc ggctgcgcat tattttcctc ttcgccctga tcctgggaac
      661 gatcgccgta cttttgcact cgctcagcaa tctcagtccc cttttcatct ataacaaaaa
      721 cgcggcgaca ccgccctggt gcctgatcag ctccgcctgg accgccctgc tcttcgcact
      781 gatctactgg atcgtcgacg gccgcgggct caccactggc acgcgcatgc tcgccactgc
      841 cggccagaac gctctcttcg ccttcatcct gggccccatc ttttacctgc tcatcggcat
      901 gcttccggtg atggcggacg gccgcagcct ctacggcatg ctgggcgccg gctttgccac
      961 cggattctgg cgatcgctga tcttcgccct cgcaggaacc tggctcaccg cagcgatgca
     1021 gcgttcagga cgttatctga ggatttgaca tgaaaaagag acccggcatc gcccttttac
     1081 tcaccctcct gctcgccacc gccctaccgg ccgcctccgg taaaaaagcc gaaccatggt
     1141 gcgccctcca ccttctcggc ttcacctcgg actcgagtct catccgcctc gaaagccagc
     1201 tgccggccct cgccgaagcg ggcgtcaata tgctcatcct cgaggttgac tataatttcg
     1261 attaccagtc ccaccccgag ttgtgcggca ccggtcaacc catcacccgc gccggggcca
     1321 aatggctcac caaggcttgc cggcgccaga acatcgagct ggtgccccag tttcagtgtt
     1381 tcggccacca atcctgggcc ggaaccacct atgccctgct cacccgctat cccgaattcg
     1441 acctcactcc gggcgcctat ccgggcaacc aggggatcta ctgccgggaa tgggaccctc
     1501 tcaatcccgc agtccatgcc atgatcttcg atctgctcga cgagttgatc aaggccttcg
     1561 acgccgacat gatccatgtg ggcatggacg aggtatttct gatcggggac agcctctcgc
     1621 cctccaccct cggcaagaat cccgcttccg tcttcgccgg ggcggtgaac gacctctatg
     1681 aacatatcgt gaaaaaacgg gacaaaggga tgatgatgtg gggagaccgc ctcatcgacg
     1741 gagagaagta caatctgggc gaatgggagg cttcgaaaaa cggcaccgcc gcagcgatcg
     1801 acagcatccc taaggatatt atcatctgcg actggcatta cgagccgcgc gaggaatacc
     1861 cctcactgcc cctctttatc gacaaggggt tccgcgtgct gccgaccagc tggaaaaaga
     1921 tcgaggcggt tcgcgccttt atcaattaca gccaggcctt gaaccatccc gacatgctcg
     1981 gccacctctt cacgatctgg agttcgaagc aggacagttt ggccctttac cccccgctgg
     2041 cgcagttcgg ccgtcaggtg cgcgccggcg caatgcctgc cgcccatagc ccgggggcgg
     2101 tcaacgctcc tgcgctgcgg tgatcacctg cagcagccgg tcgcccaggc cgacctgata
     2161 gccctgattc atccacagga tttcgccggc gggattcaca tagaccagga ccggaaaaac
     2221 cgacagctcc ccagcaaggg cctcacccag ccgcctccgc accccgtcat gctcatcctg
     2281 taccaccaac agctgcctgg gcagcgaata ggcccccaat cggccctcac cgccagccgc
     2341 catcacggcg gctatccttc ccccccatgc ctcgtagagt tgcttgagct catgccattc
     2401 gcgcaggata tgctgtgagg gttcacggcc ggggtcgagc caggcgagga tcagtccctg
     2461 cggcctggcc agctccgcaa ggtcgagccg gcgcccgcgc ctgtccgtca gcatgagacc
     2521 gccaggcagt cgggcgaggg gcttgagcgg ctccccgctg cggcgccaga ccagatcgat
     2581 cgagcgctgc cccccggggg cgaggtcgaa gaagtgcagg cgggcgagga ccgcaccgct
     2641 ctgcaggcgg cggccggtga caagaagata attgccggcg ggaagctcga gcatccccct
     2701 cccttcgctg aaatcaatcc cctccagttc cagggtttta tactccccgc cctccaggcg
     2761 ggccagggtg aaatggcgcc agtactccgg ccggctgatc gtcgcctccc ggttggccag
     2821 agtcaggcgg ctgccggcct gaatggcggc ggggtcttca ccaaaatccc gccagccctg
     2881 gtgcgacaga tcgtagtact ggggccgttc gaaggcggga tcgaggcggg cggggatgtt
     2941 catgctgcgg cagagcgcaa ccagaaagat atcccgcgaa agtgcatcag ccgcccgcag
     3001 ctcccagacg ccccggggag tcaggggcac cccgtaatag ttggcgtcag cctccaggcg
     3061 gatattcttc tcgacccagg cggccagcat cccggggctt gcgaccactg attggcctgt
     3121 ttcccggctc atgccctcct ggagcagccg gcgccagggg gtcagggcct cgttgcggac
     3181 acgcggattg agcagatatt gctcataaat cccgtcgggc agttttgtgc cggggatgtg
     3241 agagggcagg tgatcgagga ggatctccgc gcgggcgtcg cgcaggtcct tctccgtgac
     3301 catctgcagg agcgggagca cgagagagcg attgcgagtg gccccggcgc tgatgaagcg
     3361 ggcgatctcc tcgtggttgc cgcggctgct gcggatgatt cgccagaccg gatcgggctc
     3421 aagaccgctc tcagcggcga gccgcgcgct ggaaagggag tccataaagg tcgactcgca
     3481 ggccctgcgc aggctgtctt cgcgggcgag gcggcgggca tggcgcccgc gcgcctccgc
     3541 cggaatggta tcgggcaggg gcggccgggc gaggggcgga ttaaaatcga gttcaaagat
     3601 ctcctcgcgc ggggcgcgcg ggcgcagaat cacagtcaca gtatccgctc cggccgcgcg
     3661 ggaaaagccg atgagggagt cccgatgcgc ccagatcagg aggtcaccgc gcccggtgca
     3721 gaaagtgcag aatccttccg catcgctggt gcgccgcgcc agtggataga actcggcata
     3781 gttatagaga cggaaggaga cgtttgcgcc ggcaagagga cggtcggctc catcgagcgt
     3841 tctcaccacc aggggcctgg tcatggcata acgctcgagc agattgatct cacggtggcg
     3901 gggcgaagtt gcgagcacct cctcaccacc tgcccagggt ccctgcaccc gggtgtggac
     3961 gagcatggcc cgccgcgccg gttcggtgaa ccagcccata ttgaggtcag gttcgggttc
     4021 gcaggccccg agatagtacc actctccgtc ggcccagagt tccacccagg catggttgtc
     4081 gtcgctgtgc gcccagcgcg gcgtgtagac ctgacgggcg gggataccca ctgcacgcag
     4141 ggcggcgacg gtgaagaccg actcctcccc gcagcggccg atcgcgctgc gcatggtcgc
     4201 caggggggcg ctggtgcgca tgtccgaacc gcggtaggtg acatgctcgt gacaccagtg
     4261 gttgacctcg agggcggcct ggcgcatggt catgccgcgc acccgctccc tgagcaggcc
     4321 agacagcacc acccgggcgg agtcgaggtt ctcattgttg acgcgcaggg gaaggacaaa
     4381 gtgcagaaaa aggtcggccg gaagggtgcg gccccaggcc atctcctcgc gaacctgcag
     4441 ggtggtgtgc acctggcgca ggtaaaattc cccgtcatag tcggcgaggt cgctgagggg
     4501 catccaggcg tagagccact caagggctga gcgctcggcc gcagtgagct tttgcgagaa
     4561 aacgccaaag agctcctgtt ggcgaggccg ggacagctcc ttctgaattg gcagctgcgc
     4621 gtgcacggcg cggcgcagtt cgcccgctgg aatgaggctt tccccggagg gggcgcaggc
     4681 cgcccccagg atgatggtca cgaggaggag cagcttgcgc atggaggact cccggttggg
     4741 gtgaagaggc ggcgccggcc ggaacagccg ggaggggccg ccggggggga tccgccgcgt
     4801 cagacgagct tcagggtttt aatctcgaag gggccgaaat cgagtgcgac tgcgccttcc
     4861 cgctgtggca gttcgccaat ctcccgttca agcagatcgg tggcaaaaac gcgccggccg
     4921 ggatcggtga ggagcagccg ggtcgaggcg cggcgtccgc gcggctcata gaggcggagg
     4981 atgatggcat catccccctc cgctttcttg aggaccgaga ggatcgccgc ctcattttcc
     5041 agccggcagg gaaaatccac ccgtccgctc cggtcctcaa agagggcgag cggctggttg
     5101 agctgggccg cctcagcgta gactgccgat tcctccaggg cgccggcgtg ggggagcagg
     5161 ctgtagacaa aatcatgctc gccgcggtcg gcatcaggat cgggggaggt gggagcgcgc
     5221 agcagattga ggtcgatggt gcgatcgagg accttgtggc cgtatttacc attattgagc
     5281 agggcgaccc cgtgagtcgg ttcgctgagg tcaacaaagc ggtggccgac ggtctcgaac
     5341 ctggccatat cccagctggt attgcggtgg gtcgggcgcc gcaccgtgcc gtattgaatt
     5401 tcatagctgg cctcggacgc ggtgacgtcg gcatcgaagg cgacgcggag catacggtgg
     5461 cgctcccgcc aatgcacatg ggtgacgaaa tcgaggcgtt tggtgttgcg gaccaggcag
     5521 aggcgctgtt caatgaaaga gtccccgacg cggaattgca ggagcatcat ctgcgccact
     5581 ggtcccgaac ccagacggcg ccagccggtc aacccggcct gctgcaggag ctgattctcg
     5641 taatagatat cgacgtccca ggcatcccag ttggcagggc ggtcctcgta gaggctgatg
     5701 cggttgccgc tctggcccgg gagcatcact tcgcagctcc tctccttgtc gaagatgcgg
     5761 gcgatagtgc cgtcgccggc cagctcgtag aggatgagat cgttttcgag gacgacgccc
     5821 tccaatgatg cgggggccag cggctcagaa acccggggct caggcggcaa atcgacttca
     5881 ggcagggtct gcccgcggcg cagcacgact gaagagaggg ccggcacctc ggccagggcg
     5941 accaggttgc cttcgcgcac ctgggtcgga acggtctggt ccgcttcatc cagaatcaca
     6001 tggccgatcc aggcggccgg caggaaggtc ggccgcgtaa agggggtcga gagggtgttg
     6061 accagagtga ggctttcctc ctgttcgtgc aggagtccgg cgccggcgcg accggccagg
     6121 ctagtcagtt ccttcctgac ctcatcatgc tcgcggcggg tggtctcgta gaccagcttg
     6181 atgctcgagc ccggcaggat gtcgtggaac tggttgcgca tcacggtctt ccagagccgg
     6241 tccaactccg cggcggggta ctccgccgcc ggcagggcgc tgaagagggc ctcgacctcg
     6301 cgcagtttgt gttcgcacca acggttcatg aatttggtgc gcgcctgggt ggtgagggtg
     6361 ccgcgatgga gttcgaggta gagctcgccg ttccagcgct ggagcagctc acggcgggcg
     6421 ccgagccggt cgagcatctc ctgcgccttg ccgaagcgga cccgggggcc gccctcgagg
     6481 tcgttctggc gcagcccgct ctcgatgatc tcctcggtcg ctccgccgcc gccgtcgccg
     6541 atgccgaaga ggacgagaaa ttcatcgagg cggtcgcgtt cggcgaaatt ctcctgggcg
     6601 tggcgcatgc ccgagggcca gaattcgctg ttgtaggtgt cttcgggggg aaaatggacg
     6661 atgatctcgt atgccgtctt ctgcttgaaa aaaaaaaaga ggtgagggaa gcggttgaac
     6721 tggttccagg aaattttctg ggtgaccatg tacccaagac cgctcttctg gagtatctgg
     6781 ggcatggcgg cgctgtagcc gaagacgtca ggaagccaga ggttgcgcac cgtgatcccg
     6841 aactcctgct cgaaaaagcg cacgccgtgt aaaatctgcc gcaccatcga ctcgccgccg
     6901 atgaggttgc agtcggcttc gacccacatc ccgccctgta tctcccagct gccctccgct
     6961 actttagcgc gtatcttttc atagagagcg ggatagcgtt ccttgacaaa ggcatagtgc
     7021 tgggcctggg aggcaccgaa aacgtagccg ggatagcgtt cgatgaggtc gatctgggcg
     7081 gcgaaggagc gggcgcattt gcgcacggtt tccgagaccg gccagagcca gccggtgtcg
     7141 atgtgggcat ggccgacggc gtgggcaacg aggtcggaag ggccgctctt cttcaccagt
     7201 tcgacggcga ggagggcgcg ggcggcggcg gtgctgccgt cgtctccctg aaaacgatcg
     7261 gcagcctgca tcagggcgcg cagaagacgg gcgcggcgga cgcttttttc cggcagggct
     7321 ttcatctggt tgaaaagcag ccgggcgtcg agccaaaggt gccagaccga ggggcggaag
     7381 acggcgaggg tgagatcctg caccaccgcc tcgagatggc cgtggcggcg gggatcctcc
     7441 tgcaggggat cggcgaccag gcgcaggccg aagatctggg ctgcgctggc ctcaatccag
     7501 aggtcgacct gttcgccgcc gcgtgcggga tcagcgatgg gaacgcggtc gcggcggaat
     7561 tcgggcttcc agagcgaatg ccagctcagg gcgtgggtcg gggtgccgtc ggcggcgaaa
     7621 accaggccct caccgccgag gttgatgcgc gccaccaccg gccggccctg ccaggcggcg
     7681 ggcacggtgc cggtgagatg gaaccaggcg gtctgccaat ccgctcccca tttttcacca
     7741 acggcgatgg ggcggtaggc caactccagc cgccgcgcaa aggaaagggg cggatctgcg
     7801 agggcggctt cagcctcgag ggggagctgt tcggagtaaa aatatgatgg cagccggtcg
     7861 acaaactggc cgatccgggt ctcatagagc tggatttcgc gttcgatcat gcctcagcgc
     7921 actccctgcg ggggtgcggg aggcatgtcc ggcctctggc ccggcatccc tcccggctgt
     7981 ccggttctgg cctgcggctg tgcaggcgcc ttgctgagag cggccttttc ggcgttgagg
     8041 gcctcggccg ttttcttgag cgccttctca ttccactttt ccgggtactt tttacgcagg
     8101 tcggcggtca ccttctcctg cagctcggcc atcttggcac tgtagatgcg gctgcgcagg
     8161 gtcgacctca gctcggcgag ggggcgatcc ttatactccg acttgtggct ctcgtaaaag
     8221 gcggtgatct cttcttcgct caccttgact gcacgagtga tgagtttttc acggcatttt
     8281 tgaccgagga gctgattttt ggcgtagaag aaatcgcgct tgaattcctc cgtctggtcg
     8341 agcttgcgcg cgcgggcatc cagcagcatg agttcctgca ggttggcctt tctggcgaaa
     8401 tcggccacat cctcgtagcg ggtcaggcga taggcattgc cgcgcgtcat ctgcaggtat
     8461 ttgtcgaggg tttcacgcac actccactgc ccgcccttcc agctcaccag ggtctttttc
     8521 tggtcctcag gcgagatcag ggcatagtcg accttggcgc cggcactgtt gccctggagg
     8581 gcaggtgcta tcttgacgaa aaggaggctg tcgatggcaa gattgaattt gctgtagaga
     8641 tcattggtgt aattctcgac gaagagcccc tgtttctggt tcttgagatt gccttcgatt
     8701 tcggtgcgag cctgctcgaa ggggggctgc tggcgctccc gccgctcatc gatgcggaga
     8761 atctgatacc caaatccgcc aaggatgggt ttggtataag agccggtggg ggtattgcgg
     8821 accgcctcag cgctgggggg ggcgagctga ttccaggaga gccagggtgc gatgctgccg
     8881 ccgttctcga tggtattcat gtccatgctg tagcgggtaa ccagggtctt aaaatcggca
     8941 cccgccatca gggccgagta gatcgaatcg gcgagcgtgg gcgattttac cagaatgtgc
     9001 gagagtttga actcctgttt gccccattca taggcattgc gaatctcctc ctcgctgacg
     9061 ctgaaactgg cgggcacgac ggccttgaag agggggccgt tattgcgggt cagcatccgg
     9121 cgctcttcct ggcggatctg ctcagcgatg gtgctgtcct ggtccagttt gagggcattg
     9181 ccttcggcct ggaagaagag attgttgagc agggtcttct cgatgaacac cttgagaatc
     9241 tcgggggtga tggcgggatg ctgggagaac tcccgactca ttttgtaacg gcgcgtgagg
     9301 atatcgtcgc gcagggtctg atcctttacc ttgaccagcc agccctcatc ctttttggcg
     9361 cagccgatca gaaggcttgt cagcagaaag gcgaagccca gatacttttt aatcatcttt
     9421 ttctccatcc agctagagtt catttttaca taatgatctt taaaatacac attttatcgg
     9481 aaagaatcaa cggcaacgcg cgcagtcatg aactcactgc accaccagtt catcgacaaa
     9541 aatccagctc ttgccgcccg cgccgggatg ccaggcaggg caaagaccga cgttgcgggc
     9601 gcgcaggcgg acgaagcgcg ccatggtgcc cggcagctcg gtctgatcct cctggatgcg
     9661 ctgctccgag gtgccggccg gggtgggatg atcgaacgca gccacagtcc ggaaactgct
     9721 cccgtccctg gagagggacc attcgacata ggccggcaaa aagatccatg atccggtatc
     9781 ctggagaaaa ccggcgcgga ggcggtgcag gggcctgatt tctcccaggt cgatggtggc
     9841 gatcaggtcc acctcctcgt agccctgcca gcggccgtca ccgtaggcga tcgaaccgcg
     9901 caggccgtca acaagagccc ggccgccgcc ggcaggatag ttggggctga agctgtggtc
     9961 gatttgcaga ggcgccgcga aagcgagatg aaaatcgaaa tctgcacccg cactctcgcc
    10021 atagccgcgc ccgtcgcgga aggcgcgcgc cctgagccgg gttggacggt cgatggtcag
    10081 ctgggacgta taacgcggcg aggcggttgt gggttccgcg ccgtcgaggg tataatgcag
    10141 ggccatcccc tcctcgccgc tctcgagggt gaccgtatgg aatttctcat cgggatggaa
    10201 gcggctgagc aaagagaccg gcctcgcctc ggggccgaag ccgataccga gggtttcgag
    10261 cagggggtaa tgattgcgga cccgccgcag aaaatccgcg taatcgcgct gcccgggatg
    10321 gctccagagc acctcgctga gggcgagcag gcgggggaaa agctggtagt cgaggttctc
    10381 ttcggtgatg cgctcgctcc agaggcagca ttcgccgccg ataatgtagg ggatgcgctc
    10441 cggctccagt ccggccggaa ccggttcgaa actgtaggcc ttgcgcaggt cgatggcgct
    10501 gatgtcggca ttgaaatagg tatggctgcc gggcgagacg atggcgtcgt ggccggagcg
    10561 ggcagcgatc agggcgccct ccattccgcg ccaggactgc acggtggcgc cgggggccag
    10621 ccccccgtcg aggatctcgt cccagccgat gagccggcgg ccgtgggcgc tgaggtattg
    10681 ttcgatgcgg cggatgaacc agctctgcag ctccgcctca tcatcgagtc cctccgcctt
    10741 gatgcgcgcc tggcagcggg gacaggcctg ccagcggtca cggggcacct cgtcgccgcc
    10801 aatgtggaga tagccgaagg ggaagagttc gatcacctcg ctgagcacat cctcgagaaa
    10861 agcaaaggtc tcctccctgc cggcgcagta gacctccttg aaaactcccc actgggtctg
    10921 gacggcgaag gggccgccgg tgcaggagag tccgggataa gccgccaggg cggccatgca
    10981 gtggcccggc atctcgattt cggggacgac atcgatgaag cggtcggcgg catagccaac
    11041 cacctccttg atctcctcct gggtataaaa gccgccgtag cggctgccgt cgggctcggt
    11101 ccgccaggcg ccgatcccgg tcaacagtgg gtattttttg atctcgatgc gccagccctg
    11161 gtcctcggtg aggtgccagt gcaggcggtt catcttgtag agggccatcc agtcgatgat
    11221 ccgcttcata taggagacgc tgtgaaaatg gcgtccgaca tcgacgtgga ctccgcgcca
    11281 gctgaaccgg ggctggtcga tgatctcgag ggcgggcagg gcgagcgtcc gtttgccgct
    11341 cccggagggc agcatctggc gcagggtctg gccgccgcga tagagccccg cgggcgacgg
    11401 tgcggcgagg aggagctcgc gccgggagat ggtcagccgg tagccctcaa gcccggcctc
    11461 cttgagtgcc gggtcgagga ggaggcggat cggggcgctc ctgcccgcct ggctggaggg
    11521 gaggatggcc aggtcaaggc cgctgctctg gttgatctcg gcgccgagga gacgggcgat
    11581 acggccggcc tcggggctcc cggcgggata gacgatgggg gtggaggccg agaacgcaaa
    11641 gctccccttg cccgttttca ttagcacagg ctgcgggacc agagagaccg cggccgcggc
    11701 ggacgcggtc agggcgagta aaaaggagca gcacaggggt gcgaatttca tcagcttctc
    11761 catgcagtta tttcttcggc gaaggcagga ccatgttggc ctcgtccaga cgccagtgac
    11821 ggtcgaaaaa gccggcagcg gccacgccgc cgctgttctc gcgcagaatc gcactgctca
    11881 gcacggtgaa atccggcagg gccgccccgg catagagata gggcagattt tgggtcaggc
    11941 gcatccctgc gggtgcggtg ccggcgatga ccgctacaca ggccttgtcg ctgcccggcc
    12001 gcggctggat gaagagcgcc gccaggtcat cgccgcggag gatctgcttg ccgatctgga
    12061 cgatgctgcg ccggacctga atcggactct ccgccagcag atttttccag gccgcattgg
    12121 tggcggaatt gccataaagg acaaccgagc ggtcgcgatc cgcatcggga ttgaaatcaa
    12181 catcggcgac gacatcgacg ctgccgttgc cctggtacca gaaggactcg gcgtcgaagc
    12241 gggctttttg cagcgcccag cggttctctg ttgccgatcc gcgggtgccg tagacaaaga
    12301 tcatgcgctg gttaaaggcc tccttgaagg gaccaaagcg gtggggcccc ttttcatccg
    12361 cacgcagagc cggaccagcg ctccagcgcc cagccctttt ctccagccac agcgtttccg
    12421 ccccctcaag atcgatgcgc tggctgtcga gctcgacgtg gaatacggcc cggccggctc
    12481 ccgccggggt gcgcagggcg aggcgggcga tattcccggt gctcccctgg aaatggcgcg
    12541 cggccggata aaattcgatg cgggcacggc tgactgcgag aggaatctcc tgatccagaa
    12601 tggtcagcca gccgctgcgc gccgagattc cggggttggc ggtggtaaaa tcgatccggc
    12661 gcatcctctc cgcgcccgga cgggcatggc gcgcgaaaaa gtcgaagagg ggcatccagt
    12721 cgacacagtc ggcgccgggg gcgtcggaga gatcccacca gtgtcccgcc tccggctgtt
    12781 cgtggaaata ccagtcgtgg tgaaaggtct cgagctgttc gaccatgcgg cgtgactggt
    12841 cgacgcgcac gttgtcatcc ttgctgccat ggatgatata gacgccctga tggctgtaat
    12901 tcccggcgat ggcaaaggta ttgctcgggg tcgaggcgcg gcgcagcagc tgcagcgccg
    12961 ggtcgccggt gctgctggta tcgcgcacgc cgtaggacca gaaggatatc cagccggcgc
    13021 tcgggccgat ggcggcgaaa taatcgggga aggtcgcgcc gaggtgccag gtgccgtgcc
    13081 cccccatgga atggccggtc aggtagatgc gggtggtatc gatcttgagc ctgcgccggg
    13141 cgaggtcgag cacctcgagg gcgtccatgc gtccccagtc ctcccagttg tggccgtagg
    13201 gccggcggtt gctcggcgcc acgatatgcc cccagctctt gggtgcgtaa gaggcggcct
    13261 gattggcccc ctcgacattg gcgccgtgca gggagagaaa gagggccgcc ggggcgtcag
    13321 ccgggccgga agcgggattg acggcgtagt actgcacgct gccgtcgatg ccgcttatga
    13381 aggtgatgcg gtgggtggct cccgcctcga cgcggcgcag gaggatgacc gtttcatcga
    13441 gaatctcctc acccgctcca ctcctgcgca gcagggtgag agtcagcggc agctcaccag
    13501 cggccccggc cggagcggcg gaaatcggaa aggccacctt gcgcaagccg agggggagca
    13561 gctccggcag cggggtttcc gggaccttca ccccctcgcc gcggccgcgg atgaaaaggc
    13621 cgctaagggg ggcattggtg ccgttaagca ccacgatccc gcccgggctc tccagggcct
    13681 cgccggcgcg aagatcaggc agggtcaggt caaggggatt gaacagggcg gttttggtga
    13741 ggggatggag caccaccttg aggcggccgc ggctgcagcg gaaaagaaga tggttctcgc
    13801 cctcatcgag ctggatgggc accaggccgt agtcaaaacg cggctcccac gactcaaatt
    13861 ggtccttggc acggtaacgg ttgccgatgc gcggggttcc attgacgtag accatgctgt
    13921 tccccatgcc ctcgagcaga aagagggcgg gctgcaggcg cgtaatgcgg aaatggaccc
    13981 agccgttgcg cagggtggga tcgctgaacc agcccgcact gtccgccgtc actgccttcc
    14041 agctctcccc ctcctctccc tggaccagga gatcgccggc catgggggcg acctttttgc
    14101 cagcggtcac cagggcctcg accgggtcgg cagggatcat ggcgcggcgc tggtgttcgg
    14161 gaaggcggca ggccaggcca tggctgagct ggaggggctc cccctcccgg gcgagcagcg
    14221 ctgccggctg cagagccaac aaaaggatga cgagcaggcg tttcatgcgg attcctcgtg
    14281 gtttgatgaa cgggtgccgg agcggaaaga ccgggatggg cggcggccgg tgcgcaccgg
    14341 ccgcgggacg ggtgagagcc ggggccctat cccatcacca cctcgacctc caccctcttt
    14401 ttgtccgtca ggggaagcag ccgcccgctg atgggctggc cgtcgaagag gatgctgcgc
    14461 acccctctct gcacgccgtc ggggttggtg acgcggatga cgtagcgggt ggaacggaag
    14521 acgcgttcca gggtaaagcc cggccaggat gaggggatca ccggatcgac gaagaggccg
    14581 tcgaaggtgg cgcgcacccc aagacagtaa tccatcatca cccggtacat ccaggccgcc
    14641 gtgccggtct gccaggaatg gctcgcccgg ccgggggcgc tgtgttcgtc gctggtgaca
    14701 tactgggaaa agacataggg ctcggcgaca aagatatccg aatcaatggc gttgggcagc
    14761 atctctttat agtaggtgaa ggcccggtcg ccgtggccga ggaggcattc ggccatgatg
    14821 acccaggcgg tggggtgggc gaagatagcg ccgttctcct tcttgccggc aacgcagcgg
    14881 gtgaccaggc cgatagtcgg atcgatctca cggaaagcgg gcgcgcattt tttggggcca
    14941 tagggggtat tgaggtggtt ccacacgctc tccatcgcct gttcgaggcg tccggatgcg
    15001 tcgatctcgg cgatgaccgc ccacgactgg gtgttgagga agatgcggcc agccgtgttg
    15061 gtccggctgc cgatcctgcg ctcgcgggcc ccgaaggcgc ggatatacca ctccccatcc
    15121 caacagtggt tttgcaccgc ctctttgagc aggtcgcggg tctcgacggc ggtggcgagg
    15181 ggtccggcca gtccgaggtg ggtgaggagt tcaatgagat gattgagcat ggcgacgtag
    15241 aacatggcgc cccagacgct ttcgccgccg tcatcaccgc cgatatagtc gagggtgtcg
    15301 ttccagtccc cgcggccgaa gttgggcagg ccatggctcc ccagattgcc gcgcacgaag
    15361 cggaccacgg catggaggtg ttcgatgagg ggggcggcag gggcatcgat gaagggctgc
    15421 tgattgtcga ggatcgccca atcgccggtc tgcttgatgt agtcgctgat ggcgaggatg
    15481 taccagaggg ggtcgtcgca gtggccggtc acctcgccct ggccgtcgcc gtggaaatgg
    15541 tgatagacat ggccgtcgag gaacatctgg cgcgagagca ggaggatgcg ctggcgcgcc
    15601 agttccggat cggagagggt gacggcgatg gtgtcctggg cggtatcgcg aaagccaaag
    15661 ccgcggccga tgccccagta gtaaaaactc gccacacggc cgacctcaaa ggcgacccgg
    15721 gcctggtagg gcgtccagta gttcataaag atatcggcgc cgggctcggg ggtctgaatc
    15781 cgggtgacct ggaaatagcg ctcccatttg ccgcacagct tctcccaggc gcgctggcgt
    15841 gctgcgggtt catgatagcg caccggcagc accctgcaca actgcttgca ctcatccttg
    15901 gccacgacgc cgaggttaaa gaccaccggc agctcctgcc ccggcgccag ctcgatatcg
    15961 cactgcaggg cgccgatggc attgccgaaa tcggtctcct ggctggagag cctttcctgc
    16021 ccgatggcgg cgggattatt ctcgttgcga taattgccga tgaagcgctc gcgcagggtt
    16081 tcgaaggcag tcaccggctg attgacggag aagaaggccc attgatccca ctctttgttt
    16141 tcctgatgct gggtccctcg cgtaccggtg acccaatagg tcttggtggc gaaaaggctg
    16201 cgcacgctgg gatcgaaatg gacacggtta aagtgctgat cgtcgcactg gttgatcaga
    16261 tcgatcatgg catggccgag ggcgaactcc acatagccat agaccgagag gcggcggggc
    16321 cggccgctct cgttgcgcag cagggcctgc cagatctcga gatggtcgtc gggagggacg
    16381 aaatagagca ggtcggagcg gatcccctca acccgggctt cgacgcgggt gtagccgaaa
    16441 ccgtggcgga cgcggtaggc ctcgagattg cggccgaccg gctgccaggt cagactccag
    16501 atttcgcccg aatcacgatc cttgacgtag atatatttgc ccggacggtc cggcggtaca
    16561 tcattgatgc ggtagcgggt gacccggccg tgcaggggcg accggtaata gctgatgccg
    16621 ccgccgttgt tggagatggt cgcgaaatag tggccattgt agatatagtt gatccaggga
    16681 cttggagtag caacattgtc aatgatgtac tccttgccgt ccgggctgaa atcaccatac
    16741 ttcatctgcg atccctctcc tcctgggaag cggtgggcca aatcaatccg tcatgccgga
    16801 tatgccaggc ggatttcccg tccgccgctg tgagcagagc ctcccaggtc tccggtccgg
    16861 cactgagcga gggcggccat gtgcagccgg ccggccggtc cagctgcagc ttcaagccgg
    16921 accagttctt cgcatattgg cctgtatccc ggtggagctg gcgctgccgg taatagacct
    16981 gacgcaaaaa ccagcggatt tgttccgcct cgggcacatt gaactctcca cccttttcgc
    17041 cggccgggtg gctggagaat tggacaaatc cccagagttc cgggtaatgg atattaatca
    17101 atcccgtcgg ggcccagacc cagttgtcct cgggcaaact tttgccggtg gcgggatcgg
    17161 tgagcttgac gtaggcgccg ttctcaaccc gcgtgcgcca ttcgacccgg gaaaagttga
    17221 cccgccagta tgcgcccggc cgcggagcgc gcccctcggg agcctgttcc ttcagtacgg
    17281 cccagggcag ggccagctcg atcgtccagc cggtatcggc atcgccgggc tgattgatgc
    17341 tgccctgtac gaccaccccc ttctgcagtc cgcggatatc ccagaagaag gtcggattgc
    17401 cgccatcgcg gtaggggcgg tcgagataga ggtcccagtg ggtggcgaag gcgttgagtt
    17461 cgagttcgta atagcggtgg gtgtcgccgt ccggatcgat aaagacctcg aaatcgttgt
    17521 catagaaaat gatcgaatcg tgttcgcgca gcgacgccca gacgtcgggc tcctccatct
    17581 ggccggcgat gtagaaatag tggtcatccc aaagcatttt cacgcgggtg ttgaagcgcg
    17641 gccggggctt ctggtcgccc tcgatatcga caaaggcctc actccaggct gcgcgctgcc
    17701 agtcggtctc cccgagattg ccgtcgataa tgagggggcg ggtagtccgg cagcagatgt
    17761 actgcggggg agagaagggc actttgggca gctcgagcgc agagagcgcg ccggccgcgc
    17821 agaggagcag ggaaatgagg gcttttttca tgggtttcct cttgtgcaca gggctcagcg
    17881 catcagggtg atcgggcgca cctgcaggga gcgctgcccg gccgcgtcga cgccgagcac
    17941 gttgatataa taaatcccgg aagcggcagc ctgccccgca tcggtccggc cctgccattg
    18001 aaaagtgtgc agcccgcgcg ggagggtgcc gttggccacc tgcgccacca tctgtccgcg
    18061 gctgttcatg atcgaggcgc gcacctccat caactccggg acaaaaaggc ggattgccgc
    18121 gctgctgttg aagggattgg gccaggcgcc caccagttgc aggcgctcgg gctgcatccc
    18181 ctcctccaca tcctccagcc gcaccggact ggaggcgcga taatagatct cgaattcata
    18241 gatgcggctg acatccggtt tggtaagata aagccgcacc tggcggctct ctaccttttc
    18301 gggcagggcg tggtaggaga cgcttccgct ctggtcggcc ggattgtccc tgacgctaag
    18361 cgcgttgcgc cagctccccg aactgtcgag cagctggatg gtaaaatcgg cggagttcat
    18421 acccgggccg tccccggcgc cgggatcgcc ggcgggggcc ttgcccgcgc cggcgtggcg
    18481 gatgacgaaa tagttcatcg ccgcggtatc gggcagggtc agctcgagcc agtcgggtgc
    18541 gccgttattg cagcaccatt tggtggccag tgagccgtcg atcgcctttt gggcattttc
    18601 actcaccgca ttgcactcgc agtcggcgcg ggcggcggcg gtcagggcga ggttgatttc
    18661 gtccggtccc acctcaaagt cataaaaggt gaccgtgagg ggctgcgggc tggcgaggag
    18721 gccgttgaga tcggcggcgg tgaattccgt ctgcagggca ctgctgctct tcatccagat
    18781 ctgggcgctg ccagtggtga tggcgcagtc aagcgggttg gtccagacga tgccatccag
    18841 cgaaaagagg agttcagggt tcggggcaga aatccggatc tccgcggcgg cgacgaagac
    18901 cggcaggccc gaatcgtcaa ccaggtcgat gcgcacagga ccgggtgtgc ccaccggatg
    18961 aatcttgccg gactcgaaac tgatagcgat gcggctggga cgccaggagg ccacatcccc
    19021 ggcgccggaa agataggagg agacgcgcca gtagccggtg ccgctggcat cggcggggcg
    19081 gatattctcg acggcatagc ggatggtgtg aggccccggg gtcagggcag cggaaaggtc
    19141 gaggcgcagg ggatagacga tatcgccggg acaccagccg ctgcggc
//
LOCUS       DAOPWU010000023        18688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3003_length_18688_cov_4.996082, whole genome shotgun sequence.
ACCESSION   DAOPWU010000023 DAOPWU010000000
VERSION     DAOPWU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3003_length_18688_cov_4.996082"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PKM23_02600"
     CDS             <1..929
                     /locus_tag="PKM23_02600"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNY90392.1"
                     /translation="YITREYVPFEILLPAPLGDEAAPIRSWLSDKRNGAVDLIAPLRG
                     EKAQLLRMCDRNARLLLQEMQLQREKAQDFTAGSVKALQKDLLLEKLPMRIEAFDISN
                     IQGTDPVASMVCFVNGKAHRSDYRHFRIRTKSTPDDFAMMREAVGRRYSRLKSEGQAF
                     PDLILIDGGKGQLNAALEALAALEIAGQPIAALAKRLDEVFMPGESDSRYIRRDSSGL
                     RLLQRVRDEAHRFAVTFHRSLRKRRTLVSELESIPGIGKARRDALLKSFKSVKKIREA
                     GVEELSSRAGMPPRLAETIWRHYHPGEAGSGG"
     gene            complement(958..1311)
                     /locus_tag="PKM23_02605"
     CDS             complement(958..1311)
                     /locus_tag="PKM23_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90393.1"
                     /translation="MMALLAALALGLACVKKEPALPSSQIEQFAKVYIGYLTICMSDT
                     AHAEARTAYLQSELDRHQMSRTEFERIRQGLEADPQAFIDLLNRIEGGLEVKPARKAP
                     PQEADPAGQAVPIVR"
     gene            1421..2560
                     /locus_tag="PKM23_02610"
     CDS             1421..2560
                     /locus_tag="PKM23_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNY90394.1"
                     /translation="MKIFIPKESDARELRVPMIPADVEKLVKKGAQVVVESGMGISCH
                     YHDTLYEKAGAGIVHTPVEGFAMADMVLRLRKPPIEVVKLLRKDAIHVSFLDPFNSRP
                     LIQSLAEHGVNALCMEMIPRSTIAQKMDALSSQASLGGYVAAVLAAERLNRIFPMMMT
                     PAGTIQPSRVFVIGAGVAGLQAIATAKRLGARVEAFDTRPAVAEQIRSVGARFVEIDL
                     GETGQTRDGYAKALTEEQLQKQREGMARVCALSDVVITTAQVFGRKAPVIVTREMIAG
                     MKPGSLIIDLAIEGGGNVEGAVLGEEVVVNGVRIIGLDNLPGHVCIHASQMYSANLTS
                     LVTHFWDEEAKSFPLKLEDEIIQSALVTAGGEIRSELLKKHYESL"
     gene            2606..2914
                     /locus_tag="PKM23_02615"
     CDS             2606..2914
                     /locus_tag="PKM23_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNY90395.1"
                     /translation="MHGQTTPLYINLLIFMLAVFLGLGLIRRVSKLLHTPLMSLTNAI
                     SSVSVVAALIVMAEPKNRMIMIFATIAVALAATNVISGFMITERILRMFRKDQGGDAK
                     "
     gene            2911..4284
                     /locus_tag="PKM23_02620"
     CDS             2911..4284
                     /locus_tag="PKM23_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNY90396.1"
                     /translation="MIAHIVGYLYLLAAVLFILSLKWMGEVKTSRHGNWAGSLGMLIA
                     VIATMIAYKTQHLDLVLIAIAVGTLIGIPIALKLPMTAVPQRTAMSHAFGSLAVALVG
                     TAEYYQRLPGIDTFTMVVLAAEMLLGYLTFTGSTIAFLKLQEMISGKPLVYKGRNIVS
                     GLVVAVALGAAIGLVVNPTNPYFLPVLVAAALLFGFLLVMGIGGADMPTVIAILNSYA
                     GLSAAALGFVLDNQLLIVAGTLDGASGFILAILMCKAMNRSFTNVLFGGFGATTSSQG
                     AAVSGEIRPLTVEEAYLILEAASSVVFVPGYGLAVSQAQQAVRELGDLLRKNGAEVLY
                     GIHPVAGRMPGHMNVLLAEANVSYDELMEADAVNPKMPNIDVCVVIGANDVVNPAARE
                     DPSSPLWGMPIIEVDKSRTVIVLKRSMRPGFAGVENPLFFKQNTRMLFGDAKASIQAL
                     IAEFSQE"
     gene            complement(4370..6373)
                     /locus_tag="PKM23_02625"
     CDS             complement(4370..6373)
                     /locus_tag="PKM23_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNY90397.1"
                     /translation="MQHKIESGQDAGGIGTVWQRLVFLLFGVGSTLWFLVRVIPKPSR
                     AAYPCMRAAAPIASGFVVYLIGLGASVTAFRKARLQLRQARYGLALLLGSAALFFLLF
                     TIVADEAPSQAGEVAEFPLPAQASLTDQTPNRPVGLARGIFPGRVVWVHDPAATNENA
                     KPALYGSGWFLSKNNSQYVIDNMFSAALRSLTGDGSDREAWESVFRFYNRKRGLGDRG
                     YQSGEKILIKTNATSSWGGNYSTSDLSAMFNANYGISETSPQLVLSVLRHLVRTVGVA
                     QKDIYVGDPMKHIYKHCYELWYAEFPQVNYLDHDYGAEKGRLKVVPSSSASLVYSDRG
                     RIISTGPDRIYKIFDEASYMINIPTLKGHKHAGVTMFAKNHFGSHTRSNANHLHKGLV
                     ATEQGGVVNRGGYGLYRVQVDLMSHKVLGGKNLFYLMDALWSSDYEIGPPVKFRMPPF
                     NDDWTSSLFLSLDPVAIESVGYDFLFTEMEPNSGHTYSFPRMTGADDYLHQAASSDNW
                     PSGLSYDPENDGTPIASLGVHEHWNNSVDKQYSRNLGTGEGIELVRIASGTGVELTWD
                     GEHAPAGFALLPSYPNPFNASATLIFQLALPGEVELTIHDITGRRIVTLFSGSREAGE
                     CRLNWDGRSLDGTSMPSGTYFCRLQVRQQDAVYTASRRMALVR"
     gene            complement(6395..7681)
                     /locus_tag="PKM23_02630"
     CDS             complement(6395..7681)
                     /locus_tag="PKM23_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNY90398.1"
                     /translation="MKISKSSISRALRRAIAERGMIREEDTAVIFYDLSWLQGRIGEL
                     VSLFPAETLHAIAVKANPLVKVLKEIQKSGLGLEAASLPELCLAQKTGVAPDRIVYDS
                     PAKSRKELEYALQAGVHINADSLAELARIDELLKSMPLTGTIGVRINPQVGSGSISML
                     SVAGEYSKFGVPIKQQREALLSSFLRFDWLKGVHLHVGSQGCELELIMRGIETVYNFA
                     LEANRLLEREGKERRITIFDIGGGMPVAYRPDAKIPAMEEYAALLKSRLPGLFDGSFR
                     VITEFGRWLHAHSGWTASRVEYVKRDDKVNTVMIHVGADLLLRRCYRPEEWHHEIAVA
                     DRSGRLKRGRERKPFTIAGPLCFAGDIIAREVTLPPVEEGDYILIQDTGAYTLSMWSL
                     YNSRQIPRVLGYYDEGERFELLRERESLAQKVEFWG"
     gene            7900..9273
                     /gene="radA"
                     /locus_tag="PKM23_02635"
     CDS             7900..9273
                     /gene="radA"
                     /locus_tag="PKM23_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNY90399.1"
                     /translation="MNKSKKEKVHFVCQSCGNISPRWLGKCPACDSWNSFVEERAPAA
                     DVRTTGHASGVRSQPLPLEAISTDQMPRLQTISPEFNRVLGGGIVPGAVILIGGDPGI
                     GKSTLLLQEAAGLGRRDFKVLYVTGEESAQQTKMRAQRLGLDSEGLYILAETEIESVR
                     DAVAGLNPRLIVVDSIQTMYTPLLESAPGSVSQVRECALRLIQVAKSEHIPVFLVGHV
                     TKEGMLAGPKVLEHMVDALLLFEGDRDHFYRILRAAKNRFGSTREIGVFEMTERGLRD
                     VPNPSAVFLSERQTANNGSSVICCMEGTRPILLEIQALVSPSNYGLPQRTTNGIDIKR
                     LQMLLAVLEKRLGYRVGTMDVYVNAVGGFRIDETAADIGVVACIASSMRSRVIDPEAI
                     LIGEVGLGGELRSVAHIEARLAEAEKMGFKRAVIPRGNKKSLPTGTGMNIFAVSQAQE
                     ALDAVLQ"
     gene            9286..10443
                     /gene="tgt"
                     /locus_tag="PKM23_02640"
     CDS             9286..10443
                     /gene="tgt"
                     /locus_tag="PKM23_02640"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNY90400.1"
                     /translation="MSDRPFFSIVRYDRSCQARAGLLHTAHGTIQTPIFMPVGTQASV
                     KTLSPVDLNDAGAQIILNNTYHLYLRPGAELVRKAGGVQKFSAWNRPILTDSGGYQVF
                     SLSELNRITPEGFHFRSHIDGSRHFFSPEKVVEIQRDLGSNIMMVLDECAPYPCDYRY
                     ALKAHQLTIDWARRSRDAFSEIGERHGFIQHQFAIVQGSVYADLRRQSAEALIEMDFP
                     GYAIGGLSVGEPKQAMFEITGLVTALLPGNKPRYLMGVGKPEDLLEGIELGVDMFDCI
                     MPTRNGRNGTAFTSGGQIVIKNAKFREQFAPLDEACNCYTCRTFTRAYLRHLFSAQEV
                     LVLRLISLHNLHFYLGLMGRARQAILQGRYLEFKKDFLAHYHSNRHHAESS"
     gene            10463..10804
                     /gene="yajC"
                     /locus_tag="PKM23_02645"
     CDS             10463..10804
                     /gene="yajC"
                     /locus_tag="PKM23_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNY90401.1"
                     /translation="MNSVMFFLAMTAPAGQQSGGSTLGLFLPIILIIAIMYFLMFRPQ
                     AKKQKEMAQMLSALQKGDRVVTIGGIYGIVQGIKEKENIIVIKIADNVKIEVNKAAIA
                     RKLAETEGSVS"
     gene            10913..11839
                     /gene="fmt"
                     /locus_tag="PKM23_02650"
     CDS             10913..11839
                     /gene="fmt"
                     /locus_tag="PKM23_02650"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNY90402.1"
                     /translation="MRIVFMGTPDFALPSLTGLIEAGHDIPAVVTVPDKPAGRGRHLR
                     PSAVKECALGLHLALLQPGEMRDPAFIQELRGLAADLFVVVAFRILPPEVFSLPRIGT
                     VNLHASLLPRYRGAAPINWAIINGEKETGATTFFIDEKVDTGSWLLQCRVPIGEEMTA
                     GELHDLLAEEGGELLFRTVAGLDAGTLVPKPQEGAATRAPKLTREMGLIDWNRPTGAL
                     HNLVRGLSPYPAAWTRWRGRTLKIIKARPGPAGKHDKPGTLIYAGGKCPLLAATGDGA
                     LELVTLQPEGGKALSAAEFLRGHSLNPGEIIG"
     gene            11841..12602
                     /locus_tag="PKM23_02655"
     CDS             11841..12602
                     /locus_tag="PKM23_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNY90403.1"
                     /translation="MNFIKQDLQQWGRLSWQRKSAYVAAALLLSYLLLDWAIMPLYTR
                     QYQSIAVPDVINQPFNAAERRLRDLGLRAVKGSEQYDEKVAAGDVLFQVPEAGIPVKK
                     GRRIYLTVSRGQKILTMPKLVGLSERDARFALQEADLELGHVDYRTDEFYPEGVVCAQ
                     SVSPGGDIRLGTRIDLSVSAGIEPSEYIVPETVGLSLDQALQLITKAGLVKGQISEQR
                     TGKLLPNTVVSQSIEAGTVVNKGDALDLVISVLEE"
     gene            12615..13271
                     /gene="rpe"
                     /locus_tag="PKM23_02660"
     CDS             12615..13271
                     /gene="rpe"
                     /locus_tag="PKM23_02660"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013084822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNY90404.1"
                     /translation="MAIIAPSVLNADFSRLAEQITALESGGADWIHLDIMDGHFVPNM
                     TFGPMIVAAIRRITALPLDVHLMISNADAYISAFQEAGADLITVHVEACPHLWRTLEN
                     IRTLGVRPGVTLNPATPLLMIEPVLSLVEMVLVMSVEPGFGGQRYLPGAGERIAQLKR
                     WRAERSLEYLIEVDGGIDETSASGVVSAGADVLVIGQAIFRQNDIAGAVRQLRMSLQA
                     "
     gene            13399..13599
                     /locus_tag="PKM23_02665"
     CDS             13399..13599
                     /locus_tag="PKM23_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNY90405.1"
                     /translation="MARGKVKWFNENKGFGFIEQENGDDVFVHFSVIQMEGFKTLSEG
                     IEVEFDAVSGDKGLQATRVSKV"
     gene            13697..14146
                     /locus_tag="PKM23_02670"
     CDS             13697..14146
                     /locus_tag="PKM23_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90406.1"
                     /translation="MRPTFSKKDIEKISRVLGVSPKQIDNKYRFIIENNETLQHISLE
                     IYPDIPIGDHIGHLISVYTPTSHLQLHFCTGFIASDLLGEVTFVSECGGRVSGLIVEK
                     GAGCSLYANVEARLLSGDFSTMGPEVMMSGIALSLAEEILPAGLQEP"
     gene            14193..15107
                     /gene="xerD"
                     /locus_tag="PKM23_02675"
     CDS             14193..15107
                     /gene="xerD"
                     /locus_tag="PKM23_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNY90407.1"
                     /translation="MTLTDHLHSFIDFLRVERQLSGNTITAYEHDLLRYVRFLQEGGT
                     DLPENIQHHHIASFLELLHGLGLCPTSISRNVSAIRTFHKFLLSEEICSQDPTENYTV
                     PKPWMKMPRVLDLIEIEKLLQQPDTANSTGIRDRAILEFLYATGVRVSELVSFKSRDI
                     YWDDEFVRVFGKGQKERLVPVGKVALSWLREYLREVRPALASLGLGGEIVFLNRFGKK
                     LSRQTIWIMIQKYLRAAGITKKAGPHTLRHSFATHLVEGGADLRAVQEMLGHADISVT
                     QIYTHLDREFLKAVHHQFHPLESGKINL"
     gene            15104..15883
                     /locus_tag="PKM23_02680"
     CDS             15104..15883
                     /locus_tag="PKM23_02680"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNY90408.1"
                     /translation="MILPGRPRIPVIGRKVILLDRVDSTNALVKQWALAGADEGLTVI
                     AREQSAGRGRLNRIWFSPRESGLYLSTLLRPALTARESGILSLMLSLAVAKAIRSTTR
                     LRVTLKWPNDILFEGRKLCGILVEGSLNGGLLSFAVAGIGLNINQEIADFPLGLRLKS
                     TSLRLACGRPVDRDQVTVALMRHLNLLYGGLHDAGMRHKWVRVWQQHCAHMHQPVIMS
                     RGTEHIEGLFRGIDISGAALVEQETGETLLLESGEFSLREK"
     gene            15880..16656
                     /locus_tag="PKM23_02685"
     CDS             15880..16656
                     /locus_tag="PKM23_02685"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNY90409.1"
                     /translation="MNMLLVIDIGNTQIAAGIYAGKELAAHWRLSSSHERTEDETWIL
                     MQAIAAAQGFDITTVSGVAISSVVPDMTIHFEKMAVKYLHRKPVTVSHELRIGLKIRY
                     QNPANVGADRICNAVGGIARYGTPLIIVDFGTATTFDVISAEGEYLGGIIAPGIESSA
                     AILHQRAARLPRVALRFPESAIGYSTETSMQSGLMYGTVEMIDGMVERIQREMGLEVK
                     TVATGGLARVILRELRRVRQIDEHLTLDGLRLIYERVTTA"
     gene            complement(16733..16951)
                     /locus_tag="PKM23_02690"
     CDS             complement(16733..16951)
                     /locus_tag="PKM23_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNY90410.1"
                     /translation="MFDKVNDVLDTIRPALMADGGNVQLIEVKDGVVKLQLQGACHGC
                     PMSQMTLKMGIERELKRQLPEITTVESV"
     gene            complement(16997..17872)
                     /locus_tag="PKM23_02695"
     CDS             complement(16997..17872)
                     /locus_tag="PKM23_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNY90411.1"
                     /translation="MDPLMLSSIIALGALGLLFGSGLAFAAKKFAVEVDPRVEAVYQA
                     LPHANCGACGQPGCAAYADAIVAGRAAPDKCTPGGKEVAGKIASILGITGLAIAEPKL
                     AVVHCQGGHAEARERFQYQGIQDCRAALLVNGGGKACRFGCLGLGSCAAACPFGAITM
                     NSNGLPVVDEDKCTGCNVCVVTCPRAILSLIPRSQKIYLACRSLDKARAVKEVCTVGC
                     WACKICVSPKVAPTGSIVMEGNLPEIRTIDSPELLVALEKCPAGCYVYRGERPVLEAE
                     SPASAESGRTEAPAA"
     gene            18174..>18688
                     /locus_tag="PKM23_02700"
     CDS             18174..>18688
                     /locus_tag="PKM23_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/galactose cotransporter"
                     /protein_id="HNY90412.1"
                     /translation="MSVIQLSWVDWSIIALYFVFIIGIGFYLKRFTGTGEDYFLAGRK
                     NSSWVAGLAFLSANLGALEILGWTGGTMKYGMFVSHWYWIGAIPAMLFLGLYMMPFYY
                     SSKIHSVPGYLKLRFDERSRLLNAFSFAIMTLLMSGINLYLMALVLRTILGWDWHLSM
                     WISALAVGCYI"
BASE COUNT         3680 a         5770 c         5473 g         3765 t
ORIGIN      
        1 actatatcac ccgcgagtac gtgccgttcg agattcttct gccggccccg ctgggggatg
       61 aggcggcccc gatcaggagt tggctgtcgg acaagcgtaa tggcgcagtg gatctcatcg
      121 cccccctgcg cggcgaaaag gcccagctcc tcaggatgtg cgaccgcaat gccagactgc
      181 tcctccagga gatgcagctg cagcgcgaaa aggcccagga ttttacggcc ggttcagtca
      241 aggccctgca gaaggatctg ctcctggaaa aacttccgat gcgcatcgag gcgtttgata
      301 tttccaatat tcagggaacc gatccggtcg cctcgatggt ctgtttcgtc aacggcaagg
      361 cgcaccgcag cgactatcgt catttccgca tccgcaccaa atccaccccg gatgattttg
      421 cgatgatgcg cgaagccgtg ggccgccggt acagccgcct gaagagcgag ggacaagcct
      481 tccccgacct gattctcatt gacgggggca agggacagtt gaacgcagcg cttgaagccc
      541 tcgccgccct ggagatcgcc ggccagccga tcgccgccct tgccaagagg ctggacgagg
      601 tctttatgcc gggcgaatcc gattcccgtt atatccgccg cgacagctcg ggcctgcgtc
      661 tactgcagcg ggttcgcgat gaggcccacc gctttgcagt cacctttcat cgctccctgc
      721 gcaagcgccg gaccctggtt tcagaactgg aatcgattcc gggaatcggc aaggcgagaa
      781 gggatgccct gctcaagtcg ttcaaatcgg tgaagaagat cagggaggct ggtgtggagg
      841 agttgagcag ccgtgcgggc atgccgccca ggctggctga aaccatctgg cgtcactatc
      901 atccaggcga ggcgggcagc ggaggataga aagggccggc gggggagagg cagctgatca
      961 gcgcacgata ggcacggctt ggccggccgg atcggcctcc tggggagggg cttttctcgc
     1021 cggtttgacc tccagtccac cctcgatgcg gttgagcaga tcgatgaagg cctgaggatc
     1081 ggcttccagc ccctgccgta tgcgttcaaa ttcggtgcgg ctcatctggt gccggtcgag
     1141 ttcgctctgc agataggctg tacgcgcttc ggcatgggcc gtatcgctca tgcagatggt
     1201 cagatatccg atataaactt tggcgaactg ctcgatctgg ctcgacggca gggcgggttc
     1261 cttttttaca caggcgaggc cgagcgcaag cgcggccagc agtgccatca tctttatccg
     1321 gttcatctgt tccctttaaa tatggatgcc agaatatagg catctccagc catgaagtca
     1381 aacggttttc ctttcatgcc ttcgcagagg agagcatccg atgaaaatct tcattcccaa
     1441 agagagtgat gcccgcgaat tgcgggtgcc catgattcct gcggatgtag aaaagctggt
     1501 taaaaagggc gctcaagtcg tcgtcgagag tggcatgggc atttcctgtc actaccacga
     1561 caccctgtac gagaaggccg gggcgggaat cgtgcacacg ccagtggaag gttttgccat
     1621 ggccgacatg gtcttgcgtt tgcgcaaacc acccatagag gttgtcaagc tgctgcgcaa
     1681 ggatgccatc cacgtcagtt ttctcgatcc cttcaattca cgaccgctta tccagtccct
     1741 ggctgagcac ggggtcaatg ccctctgtat ggagatgata ccgcgttcga ccatcgccca
     1801 gaagatggac gccctcagct cgcaggccag tctgggcggc tatgtcgctg ccgttctcgc
     1861 tgccgagcgg ctgaacagga tttttccgat gatgatgacg cccgccggaa caatccagcc
     1921 cagccgtgtt ttcgtcatcg gcgccggcgt ggccggactg caggcgatcg ccacggccaa
     1981 gcgtctcggt gcgcgggtgg aggcctttga tacccgcccc gccgtagccg aacagatccg
     2041 ttcagtgggg gcgcgctttg tcgagatcga tctcggcgag actggacaga ccagggacgg
     2101 ttatgcgaag gccctcaccg aggagcagct gcaaaagcag cgcgagggca tggccagggt
     2161 ctgcgcccta tcggatgtgg tcatcaccac cgcccaggtt ttcggccgca aggctccggt
     2221 catcgtcacc agggagatga tcgcgggaat gaagccgggc agcctgatca tcgatctcgc
     2281 catcgagggg ggcggcaacg tcgagggggc cgtcctcggc gaggaggtgg tggtcaacgg
     2341 ggtgcgcatc atcgggctcg acaatctgcc cggacacgtc tgcatccatg ccagccagat
     2401 gtattccgcc aacctgacta gcctggtgac ccatttctgg gatgaggagg ccaaaagctt
     2461 ccccctcaag ctggaagatg agatcattca gtccgctctc gtcaccgccg gcggcgagat
     2521 tcgcagcgaa ctgctcaaga agcattatga aagcctgtag aaaaccggca cgtttttatg
     2581 ttgcgcatcc tggaggaatt atcccatgca tggtcaaacg acaccgcttt acatcaacct
     2641 gcttatcttc atgctggcgg tttttttggg attgggcctg atccggcggg tctcaaagct
     2701 gctgcatacc ccgctgatgt ccctgaccaa cgccatctcc tcggtctccg tagtcgcggc
     2761 gctgattgtc atggctgagc ccaaaaaccg gatgattatg atcttcgcga ccatcgccgt
     2821 cgctctggca gccaccaacg tcatcagcgg cttcatgatc acagagcgca ttctgcgcat
     2881 gttcaggaag gatcagggag gagacgccaa atgatagccc acatcgtcgg ttacctttac
     2941 ctcctcgctg cggtcctttt catcctctcc ctcaagtgga tgggcgaggt taaaacctct
     3001 cgccacggca actgggccgg aagcctgggc atgctcattg cagtgatcgc gaccatgatc
     3061 gcctacaaga ctcagcatct cgatctggtc ctcatcgcca tcgccgtcgg caccctcatc
     3121 ggcataccca ttgccctcaa gctgccgatg accgccgtgc cgcagcggac ggccatgtct
     3181 cacgccttcg gttcgctcgc cgtcgccctg gtcggcacgg cggagtacta tcagcgtctg
     3241 cccgggatcg acaccttcac catggtggtt ttggccgccg aaatgctgct gggatatctg
     3301 accttcaccg gttcgaccat cgcctttctc aagctgcagg agatgatctc cggcaagcca
     3361 ctggtctaca agggccgcaa catcgtcagc ggcctggtag tggctgtggc cctgggcgcg
     3421 gccatcggac tggtcgtcaa tcccaccaac ccttatttcc tgccggtgct ggtcgcagcc
     3481 gcgcttctct tcggattttt gctcgttatg ggcatcggcg gcgctgatat gcccaccgtc
     3541 atcgccatcc tgaactccta tgccggactc tcggccgcag cccttggctt cgtccttgac
     3601 aaccagcttc tcatcgtcgc cggcactctc gacggggcct cgggattcat tctcgccatt
     3661 ctcatgtgca aggcgatgaa ccgctccttc accaacgtcc ttttcggcgg attcggcgcc
     3721 accacctcca gtcaaggggc ggcggtcagc ggtgaaatcc ggcccctgac agtcgaagag
     3781 gcctatctga ttctggaggc cgcctcttcg gtggtatttg tgcccggcta tggactggct
     3841 gtttcacagg cgcaacaggc tgttcgcgaa ctcggcgacc ttctccgcaa gaacggcgct
     3901 gaggtcctct acggcatcca tccggtggcg ggccgcatgc ccggccacat gaatgtgctg
     3961 ctcgcggagg ccaacgtttc ctacgacgag ctcatggaag ccgatgccgt caatcccaag
     4021 atgcccaaca tcgacgtctg tgtggtcatc ggggctaatg atgtcgtcaa tccggccgcc
     4081 agggaggatc cctcctcgcc cctgtggggc atgcccatca ttgaggtgga caaatcccgc
     4141 acggtcatcg tgcttaaacg cagcatgcgt ccaggtttcg ccggcgtgga gaatcccctc
     4201 ttcttcaaac aaaacacccg catgctcttc ggcgatgcca aggcttcgat ccaggccctc
     4261 atcgccgaat tctcgcagga gtaggaaaac gatacctggt ggtgcatttt gctctcgacg
     4321 ctgcacaaaa aagcccggat tcccgggctt gtttttgtgc gcatttgctt catcgcacca
     4381 gggccatgcg ccgggaagcg gtatatactg catcttgctg ccggacctgc aggcggcaga
     4441 aataggtgcc gcttggcata ctggtgccat ccaggctgcg gccgtcccag ttcagccggc
     4501 attcaccggc ctccctgctg ccgctgaaga gggttacgat cctccggcca gtgatatcat
     4561 ggatggtcag ctcgacttca cctgggagtg caagctggaa gatgagggtg gcggaggcat
     4621 tgaagggatt gggatagctg ggcagcaggg caaagccggc gggggcgtgc tcaccgtccc
     4681 aggtcaattc cacaccagtg ccgctggcga tccggaccag ctcgatcccc tcgcctgtgc
     4741 caaggttgcg cgaatactgc ttgtcgacgc tgttgttcca gtgctcatgc acgccgaggc
     4801 tggcaatagg ggttccgtcg ttctccggat cgtagctcaa gccggacggc cagttgtcgg
     4861 agctcgctgc ctgatggaga tagtcgtcag caccggtcat acgggggaaa ctgtaggtat
     4921 ggccgctgtt cggctccatc tctgtaaaaa ggaaatcata gccgaccgat tcgatggcaa
     4981 cgggatcgag ggagagaaag agcgaggagg tccagtcgtc gttgaagggg ggcatcctga
     5041 atttaaccgg cgggccgatc tcgtaatcgg agctccagag cgcgtccatg aggtaaaaga
     5101 ggttcttgcc gcccagcacc ttgtggctca taaggtcgac ctggacccga tagaggccgt
     5161 agcctccacg gttgacaacg ccaccctgct ctgtggccac cagtcccttg tgaagatggt
     5221 tggcgttgct gcgggtgtgg gagccaaagt gattcttggc gaacatggtg accccggcgt
     5281 gtttgtgccc cttgagggtc gggatgttga tcatgtagga ggcctcatcg aagatcttgt
     5341 agatccggtc cggcccggtg ctgatgatcc tgccccggtc ggagtagacg agggaggcgg
     5401 atgaggaggg caccaccttg agccttccct tctcagcgcc atagtcatgg tccaggtagt
     5461 tgacctgggg gaattcggcg taccacagct catagcagtg tttataaatg tgcttcatcg
     5521 ggtcgccgac ataaatatcc ttctgggcga cgcccaccgt ccggaccaga tggcgcagaa
     5581 cgctcagaac cagctggggg gaggtctccg aaatgccata attggcgttg aacatggctg
     5641 agaggtcgga agtgctgtaa tttcctcccc atgaactggt ggcgttggtc ttgatgagga
     5701 ttttttcgcc ggactgataa ccccggtcac ccaggccgcg ctttcggttg tagaacctga
     5761 aaacagattc ccaggcctcg cggtcactgc catcgccggt cagggatctc agggcggcgg
     5821 agaacatgtt gtcgatgacg tactggctgt tgtttttgct gagaaaccag ccgctgccgt
     5881 agagggccgg tttggcgttt tcgttggtgg ccgcgggatc gtgtacccag accacccggc
     5941 cggggaaaat gccccgggcg agaccgaccg gacgattggg ggtctggtcg gtcagtgagg
     6001 cctgcgccgg cagggggaac tcagctactt cccccgcctg agagggggct tcatcggcca
     6061 caatggtgaa aaggagaaaa aagagggcgg ccgaacccag gagcagggca agcccgtagc
     6121 gggcctggcg cagctggagt cgggccttgc ggaaggcggt caccgacgcc ccgaggccga
     6181 tcaggtagac cacaaagccg gaggcgatgg gcgccgccgc acgcatgcag ggataggcgg
     6241 cccggctggg cttggggatg acccggacca gaaaccagag ggtcgatccg accccgaaca
     6301 gaaggaaaac cagacgctgc caaacggttc cgattccccc ggcgtcctgc ccggactcta
     6361 ttttgtgctg catgaccaac tcctttcctc tcctttaccc ccagaattca accttctgcg
     6421 ccagagattc gcgttcccgc aagagttcga agcgctcccc ttcatcgtaa tagccgagca
     6481 cgcgggggat ctggcggctg ttgtacaggg accacatgct cagggtatag gcgccggtgt
     6541 cctggatgag gatgtaatcg ccttcctcga ccggcggcag tgtgacctcc cgggcgatga
     6601 tgtcaccggc gaagcagagg ggaccagcaa tggtgaaggg cttgcgttcc cggccccgtt
     6661 tgaggcggcc actgcgatcg gccaccgcga tctcgtgatg ccactcttcc gggcggtagc
     6721 atcggcgcag cagcaggtcg gccccgacgt ggatcatgac ggtattcacc ttatcgtccc
     6781 gtttgacata ttccaccctg ctggcggtcc agccgctgtg ggcatggagc cagcggccga
     6841 actcggtgat cacccggaag gaaccatcga agaggccggg caggcgcgac tttaagaggg
     6901 cggcatactc ctccattgca gggattttcg cgtcaggtcg gtaagctacc ggcatgcctc
     6961 cgccgatgtc aaatatggtg atgcgtctct ccttgccttc gcgctcgagc agccgattgg
     7021 cctccagggc gaaattgtag accgtttcga tgcccctcat gatgagctcg agctcgcagc
     7081 cctgcgatcc tacatgcaga tgcaccccct tcagccagtc aaagcggaga aaggaggaga
     7141 gcagggcctc gcgctgctgc ttgatcggca ctccaaattt ggaatactcc cccgccacgc
     7201 tcagcatcga gatcgatccg gaacccacct ggggattgat gcgcaccccg atggtgccgg
     7261 tcaggggcat gctcttgagc agctcatcga tgcgcgcgag ctccgccaaa gagtccgcat
     7321 tgatatgcac gcctgcctgc agggcgtatt cgagttcctt gcggctcttg gccggggaat
     7381 catagacgat gcggtcaggc gccacccccg ttttctgggc cagacagagt tcgggcagac
     7441 tcgccgcttc cagtcccagc ccggactttt ggatctcttt aagcaccttg accagcggat
     7501 tggccttgac cgcgatggcg tggagggtct ccgccggaaa gagcgagacc agttccccga
     7561 tccgtccctg cagccacgaa agatcgtaaa agatgacggc tgtatcctct tccctgatca
     7621 tgccgcgctc agcgatggcc cggcgaaggg cccggctgat gctcgatttg cttattttca
     7681 ttctcacttc cggctggaac tgcatgctcc gctgccaggt caacgcagtg gagacctcca
     7741 tttgcacctc tcaatgtaga gtattaacgc acccaaatca aggctaatct gcaggaatgg
     7801 ggagccatac acctcgtcgc cccgatagtt atcgctgaaa aataataact tagcaaaaat
     7861 ggcttgactt tttgccatta tttcgatact ttttgcagca tgaataaatc caaaaaagag
     7921 aaggtgcatt tcgtctgcca gagctgcggc aacatctcgc cgcgctggct gggcaagtgc
     7981 ccggcctgcg actcctggaa cagctttgtc gaggaacgcg ctccggccgc tgatgtgcgc
     8041 acgaccggac atgcgagcgg cgtcaggagc cagcccctgc ctcttgaggc gatctcgact
     8101 gaccagatgc ctcgactcca gaccatcagt cccgaattca accgggtcct tggcggaggc
     8161 attgtccccg gcgccgttat cctgatcggg ggagatcccg gcattggcaa atcgaccctg
     8221 ctgttgcagg aggccgccgg acttggacgc cgggatttca aggtgctcta cgtgaccggc
     8281 gaagagtccg cccagcagac caagatgcgc gcccagcgcc tcggactcga ctccgaaggg
     8341 ctctatatcc tcgccgagac cgaaatcgag agcgtccgcg atgccgtcgc cggcctcaat
     8401 ccccggctca tcgtcgtcga ctccatccag accatgtata cccctttgct tgagagcgcc
     8461 ccgggcagcg tcagccaggt tcgcgagtgt gccctgcgcc tcatccaggt cgccaagagc
     8521 gagcacatcc cggtcttttt ggtcggccat gtcaccaagg aagggatgct cgccggtccc
     8581 aaggtacttg aacatatggt cgatgccctc ctgctcttcg agggcgaccg tgaccacttt
     8641 taccgcatcc tccgcgccgc caaaaaccgc ttcggttcca cccgcgagat cggggttttt
     8701 gagatgaccg agcgggggct gcgcgatgtc cccaatccct cggctgtctt cctctctgaa
     8761 cgccagaccg ccaataacgg ctcctcggtg atttgctgta tggaggggac ccgaccgatc
     8821 ctgctggaaa tccaggccct ggtgagcccc tccaactacg gactgcccca gcgcaccacc
     8881 aacgggatcg atatcaagcg gctgcaaatg ctcctggccg tgctggaaaa gaggctgggc
     8941 taccgcgtcg gcaccatgga cgtctatgtc aacgccgtcg gcggatttcg catcgacgaa
     9001 accgcggcgg atatcggcgt ggtcgcctgc atcgcttcga gcatgcgcag ccgcgtcatc
     9061 gatcctgaag cgatcctcat cggcgaggtc ggccttggcg gggaactgcg ttccgtcgcc
     9121 cacatcgaag cccgcctggc cgaggcggaa aaaatgggct tcaaaagggc cgtgatcccg
     9181 cgcggcaaca agaaaagcct gccgacaggc acaggcatga acatcttcgc cgtcagccag
     9241 gcccaggagg ccctcgatgc cgttctccag tgagcagtgg acacaatgag cgaccgcccc
     9301 tttttcagca tagtcaggta tgaccgcagc tgccaggctc gcgcgggcct gctgcacact
     9361 gctcacggga ccatccagac cccgatcttc atgccggtgg gaacccaggc ctccgtgaag
     9421 accctgtcgc ccgtcgacct gaacgacgcc ggggcgcaga tcattctcaa caatacctat
     9481 cacctctacc tgcggcccgg tgccgagctg gtgcgcaagg ccgggggtgt gcagaaattc
     9541 agcgcctgga accgccccat cctcacggat agcggcggct accaggtttt cagcctctcc
     9601 gaactaaatc gtataacccc ggagggtttt catttccgct cccatatcga tggctcgcgc
     9661 cactttttca gcccggagaa ggtggtggag attcaacgcg atctcggatc gaatattatg
     9721 atggttctgg atgagtgcgc cccctatccc tgcgactacc gctacgccct caaggcccat
     9781 cagctcacca ttgactgggc gcgtcgcagc cgcgacgcct tttcggaaat cggggaacgc
     9841 cacggcttca tccagcacca gttcgccatt gtccagggca gcgtctacgc cgatctgcgc
     9901 cgccagagcg ccgaggccct gatcgaaatg gattttcccg gctatgccat cggcggactc
     9961 tccgtgggcg agcccaagca ggcaatgttc gagatcaccg ggctcgtcac cgccctgctg
    10021 cccgggaaca agccccgcta cctgatgggg gtcggcaaac ccgaggacct gctcgagggc
    10081 atcgaactcg gggtggacat gttcgactgc atcatgccca cccgcaacgg ccgcaacggc
    10141 accgctttta cttcgggcgg acagattgtc atcaagaacg ccaaatttcg cgagcagttc
    10201 gccccgctcg acgaggcgtg caattgctat acctgccgca ccttcacccg cgcctacctg
    10261 cgccatctct tcagcgctca ggaggtgctg gtgctccgcc tcatcagctt gcataatctc
    10321 catttctacc tggggctgat gggccgcgcc cgccaggcga tcctgcaggg acgctatctc
    10381 gaattcaaaa aggattttct ggcccattat cattccaacc ggcaccatgc cgaatcatca
    10441 tgacccatca aaggaggtat tcatgaactc agtgatgttt tttctcgcca tgaccgcacc
    10501 ggcaggacag cagagcggtg gcagcaccct gggcctattc ctgcccatca tcctcatcat
    10561 cgccatcatg tatttcctga tgttccggcc ccaggccaag aaacagaagg agatggccca
    10621 gatgctcagc gccctgcaaa agggggatcg ggtcgtgacc atcggcggca tctacgggat
    10681 cgtccagggc atcaaggaaa aggaaaatat catcgttatc aagattgcgg acaatgtcaa
    10741 gatcgaggtc aataaagcag cgattgcccg gaaactggct gagaccgagg gtagcgtttc
    10801 ctgatccggc ctttcgtacc gttacgtccg gggtctcatc ggactgcagc agcctgatgc
    10861 agccccgttg cactccgggc cggcggcgcg gccgatgatg aaagcaatag tcatgagaat
    10921 cgtctttatg ggtacgcctg atttcgccct ccccagcctg accgggctaa tcgaggccgg
    10981 ccacgatatc cccgccgtgg tcacggtgcc cgacaagccg gccggtcgcg ggcgccatct
    11041 gcgcccatct gctgtcaagg agtgcgctct cgggctccat cttgcactgc tgcagcccgg
    11101 ggagatgcgc gatcccgcct tcattcagga actgcgcggg ctcgctgcgg atctcttcgt
    11161 ggtggttgca ttcagaatcc tgccgcccga ggtcttttcc ctgccccgga tcggcaccgt
    11221 caacctccac gcctcgctgc tgccccggta ccgcggagcc gcacccatca actgggccat
    11281 tatcaacggc gagaaggaga ccggtgcgac caccttcttc atcgatgaaa aggtcgatac
    11341 cggcagctgg ctgctgcagt gccgggtgcc gatcggagag gagatgaccg ccggagagct
    11401 gcatgacctc ctcgccgaag aaggggggga gcttttgttc cggactgtcg ccgggctcga
    11461 tgcgggcacg ctcgtgccca aaccgcagga gggggcggcg acgcgtgcgc ccaaactgac
    11521 ccgggaaatg ggcctgatcg actggaacag gcccaccggc gcgctccaca atctggtgcg
    11581 cgggctctcg ccctatccgg ccgcctggac gcggtggcgc ggcagaaccc tcaagatcat
    11641 caaagcgcgc cccggacccg ccgggaagca tgacaaacct ggaaccctga tttacgcagg
    11701 gggcaagtgc cccctcctgg ccgccaccgg cgacggcgcg ctggaacttg tcaccctaca
    11761 acctgaagga ggaaaggccc tctccgccgc cgagttcttg cgcggacaca gcctgaatcc
    11821 cggagaaatc attggctaat atgaatttca tcaagcaaga ccttcagcag tgggggcgcc
    11881 tctcctggca gcgcaagagc gcctacgtgg cggccgccct gctcctctct tatctgctcc
    11941 tcgactgggc gatcatgccc ctctacaccc ggcaatatca gtccatcgct gtccccgatg
    12001 tcatcaacca gcccttcaat gccgcagagc ggcgcctgcg cgacctcgga ttgcgggctg
    12061 tcaagggaag cgagcagtac gatgaaaagg tcgccgcggg cgatgtgctc tttcaggtgc
    12121 cggaagcggg aatcccggtc aagaagggcc gccgcatcta cctgacggtc agtcgtggac
    12181 aaaagatcct caccatgccc aagctggttg gtctctccga gcgcgatgcc cgcttcgccc
    12241 tgcaggaagc ggatcttgaa ctgggccatg tcgactatcg caccgatgag ttttatccgg
    12301 aaggggtagt ctgcgcccaa tccgtcagcc cgggtggcga tatccgcctt ggtacccgca
    12361 tcgacctcag cgtcagcgca ggcatcgagc catccgagta catcgtcccc gagactgtcg
    12421 gcctctcgct cgatcaggcc cttcagctga tcaccaaggc cggtctggtc aaggggcaga
    12481 tcagcgaaca gcgcaccggc aaactgctgc ccaataccgt ggtcagccag tcgatcgaag
    12541 cgggcacagt ggtcaacaag ggtgatgccc tcgatctggt catcagcgtg ctcgaagagt
    12601 aggggggagg ttctatggcc atcatcgcac catcggtgct caacgccgat ttcagccggc
    12661 tcgctgaaca gatcaccgcc ctcgagagcg ggggggcgga ctggattcat ctggacatca
    12721 tggatggcca ctttgtgccc aacatgacct ttggtccgat gattgtggcg gcgatccggc
    12781 ggatcaccgc cctgcccctc gatgtccatc tcatgatcag caacgccgac gcctatatct
    12841 ccgcctttca ggaagccgga gccgatctca tcacggtaca tgtcgaggct tgtccccacc
    12901 tctggcgcac tcttgaaaat atccgcactc tcggcgtcag gcccggcgtc accctcaatc
    12961 ccgccacacc cctgctgatg atcgagccgg tcctctccct ggtggagatg gtgctggtca
    13021 tgtccgtcga acccggcttc ggcggccagc gctacctgcc cggcgcgggt gagcgcatcg
    13081 cccagctcaa gagatggcgc gcagagcgga gcctcgagta cctgatcgag gtggatggcg
    13141 gcatcgacga gacgagcgcc agcggggtgg tttcagccgg cgccgatgtc ctggtgatcg
    13201 gacaggcgat ttttcgccag aatgacattg ccggggcggt ccggcagctg cgaatgagtt
    13261 tgcaggcctg aagaaaacgc ttgactaaaa aacatggttt acctacatta acgccaagag
    13321 aaagcgcttc tgccctcggg cggctcatgc tttggcagaa gttcttctcc gcgttatttg
    13381 tcaggaggag cacgcaggat ggcaagaggc aaggtcaagt ggtttaatga aaacaagggg
    13441 tttggattca ttgaacagga aaatggcgat gatgttttcg tccacttttc tgtgatacag
    13501 atggaaggat tcaagactct ttctgaaggc atagaggtgg aattcgacgc cgtctccggc
    13561 gacaaaggat tgcaggcgac cagagtgagc aaggtttgac ggacaggcct gccgtttgaa
    13621 accccgcggt tatgtggggt ttttttgtaa cctggaaagc gatcctgcgt aaacaggaac
    13681 aaaaaaggta ctctttatgc gtccgacatt cagtaaaaaa gatattgaaa aaatcagccg
    13741 ggtgctcggg gtttccccca agcagatcga caataaatat cgctttatca tcgaaaacaa
    13801 cgaaaccctc cagcatatct ccctcgaaat ctatcccgat atcccgatcg gcgaccatat
    13861 cggccacctc atcagcgtct acaccccgac ctcccacttg cagctccact tttgcaccgg
    13921 ctttatcgcc agcgacctgc tcggcgaggt cacctttgtc agcgaatgcg ggggacgggt
    13981 ctcgggactg atcgtcgaaa agggggccgg ctgctccctc tacgccaatg tcgaagcccg
    14041 gctgctctca ggggatttca gcaccatggg accggaggtg atgatgtccg gcatcgccct
    14101 ctccctggcg gaggagatcc tgccggccgg cctgcaggaa ccataagccg ccctctccag
    14161 gccaatctga tcccccctga ccgggaatgg ctatgacgct caccgatcac ctccatagtt
    14221 tcatcgattt cctgcgtgtg gagcgccagc tctctggcaa taccatcacc gcctatgaac
    14281 atgatctgct ccgttatgtc cgttttctcc aggagggcgg cactgatctg cccgaaaaca
    14341 tccagcatca ccacatcgcc tcctttctcg agctcctcca cgggcttggc ctctgtccca
    14401 cgtcgatttc gcgcaatgtc tcggccattc gcacctttca caaatttctc ctctccgagg
    14461 aaatctgcag ccaggatccc acggagaact atactgtgcc caagccctgg atgaagatgc
    14521 cgcgggttct ggatctcatc gagattgaaa agctcctgca gcagcccgat acagcaaatt
    14581 ccaccgggat ccgagatcgc gccatcctcg aatttctcta cgccacggga gtgcgggtct
    14641 ctgaactggt ctccttcaaa agccgcgaca tctattggga tgatgaattt gtgcgcgttt
    14701 tcggcaaggg ccagaaggag cggcttgtcc cggttggcaa ggtcgccctc agctggctgc
    14761 gggagtattt gcgcgaggtg cggccggcgc tggccagtct cggcctcggg ggtgaaatcg
    14821 tcttcctcaa ccgcttcggc aagaagctca gccgccagac catctggatc atgatccaga
    14881 agtacctgcg tgcggcggga ataaccaaga aagccgggcc ccataccctg cgccactcct
    14941 tcgccaccca tctggtggag gggggagccg atctgcgcgc tgttcaggaa atgctcggcc
    15001 acgctgacat ctcggtaaca caaatctata cccacctcga tcgtgagttc ctcaaggcgg
    15061 ttcaccatca gttccatccc ctcgaatccg gaaagataaa cctatgattt tgcccggccg
    15121 tcccaggatt ccggtcatcg gacgcaaggt gatcctcctc gaccgggtcg attcgaccaa
    15181 cgcactggtc aaacagtggg ccctcgccgg cgccgatgaa ggcctgaccg tcatcgctcg
    15241 cgagcagagc gccgggcgcg gccgcctgaa ccggatctgg ttttccccgc gagaatcagg
    15301 gctctatctt tccacgctgc tgcgccccgc cctgacggcg agggagagcg gtatcctctc
    15361 gctgatgctc tccctggctg ttgccaaggc gatccgcagt acgacgaggc tgagggtcac
    15421 cctcaaatgg cccaatgaca tactctttga ggggcgcaaa ctctgcggca tcctggtgga
    15481 aggctctctg aacggaggcc tgctgagctt tgctgtcgcc ggtatcggcc taaacatcaa
    15541 ccaggagatc gccgatttcc cgctcgggtt gcggctgaag agcacttctc tgcgactcgc
    15601 ctgcggccgt cctgtggacc gggatcaggt gaccgtcgcg ctcatgcgcc atctcaattt
    15661 actttacggc ggattgcacg atgccgggat gcggcataaa tgggtccggg tctggcagca
    15721 gcactgcgcg cacatgcatc agcccgtcat catgagcagg ggcactgagc acatcgaagg
    15781 tctttttcgc gggatagata tttctggagc ggctctggtc gaacaggaga ccggtgaaac
    15841 cctcctgctg gaaagtggtg aatttagtct cagggagaaa tgaacatgct gctggtcatc
    15901 gacatcggca atacccagat cgctgccggg atctacgcag gcaaggagtt ggccgcccac
    15961 tggcgcctct cgagcagcca tgagcgcaca gaggatgaaa cctggattct catgcaggcc
    16021 atcgccgcgg cgcagggatt cgacatcacg acggtctcag gcgtggcgat ctcctcggtc
    16081 gtccccgaca tgaccatcca ttttgaaaag atggcggtca aatacctcca tcgcaagccg
    16141 gtgaccgtct cccacgaact caggatcggt ctcaaaatcc gctaccagaa tcccgccaac
    16201 gtcggcgccg atcgcatctg caacgccgtc ggcggcatcg cccgctacgg cacgcctctc
    16261 atcatcgtgg atttcggcac ggccactacc ttcgacgtca tctccgccga gggggagtac
    16321 ctcggcggca tcatcgcccc gggtatcgaa agctcagcag ccattttgca ccagcgcgca
    16381 gcgcgcctgc cgcgggtggc cttgcgcttt ccggagagcg cgattggcta ctcgaccgaa
    16441 accagcatgc agtccggcct gatgtacggc actgtcgaga tgatcgacgg catggtcgag
    16501 cgcatccagc gcgagatggg cctcgaggtc aagacagtgg ccaccggcgg cctggccagg
    16561 gtcatcctgc gcgagctgcg ccgggtccgg cagatcgacg aacatctgac cctcgacggc
    16621 ctgcgtctga tctatgaacg ggtcaccact gcgtgacggg cgctccgggg ccaggtcata
    16681 aaaaaagcgg cgttttcagg acgccgcttt tttttatacc ggggagtacc gtttaaaccg
    16741 actcgacggt ggtgatctcg ggcagttgtc gcttcagctc cctctcgatg cccatcttga
    16801 gggtcatctg cgacatcggg cagccgtgac aggccccctg cagctggagc ttgacaaccc
    16861 catccttcac ctcgatgagt tggacgttgc cgccatcggc catcagggcc gggcggatcg
    16921 tatcaagtac atcattaacc ttatcgaaca tgggatcgga catttcaaac ctctctctct
    16981 tgtctgtgtg gaaggatcag gcagctggtg cctcggtgcg gccgctttct gccgatgcag
    17041 ggctctccgc ttcgagaacg gggcgctcgc ctctgtaaac atagcagccg gccggacatt
    17101 tctcgagtgc gaccagcagc tcgggtgaat caatagtgcg gatttcgggc agattgccct
    17161 ccataacgat agagccggtg ggcgcgacct tgggcgaaac gcagattttg caggcccagc
    17221 agccgactgt gcagacctcc ttgaccgccc tggccttgtc gagggatcgg caggccagat
    17281 agatcttctg gctgcggggg atgagggaga ggatggcgcg cgggcaggtg acgacgcaga
    17341 cattgcaccc tgtgcacttg tcctcatcaa cgaccggcaa accgttgctg ttcatggtga
    17401 tggcgccgaa ggggcaggcc gcagcacagg agccgagccc cagacaacca aagcggcagg
    17461 ccttgccgcc gccgttgacg agcagggcgg cgcggcagtc ctgtatgccc tgatactgga
    17521 agcgttccct ggcctcggca tggccgccct gacagtggac caccgccagt ttgggttcgg
    17581 cgatggccag tccggtgatc cccagaatgg aggcgatttt gccggcgacc tccttgccgc
    17641 caggcgtgca cttgtccggc gcagcccgac cggccacgat ggcatcggca taggccgcgc
    17701 atcccggctg gccgcacgcc ccgcaattgg catgcggaag ggcctggtag accgcctcca
    17761 cgcgcgggtc gacctccacc gcgaattttt tggcagcaaa ggccaaccct gagccgaaaa
    17821 gaaggcccaa agcacccagt gcaatgatcg atgaaagcat gaggggatcc atatcagcct
    17881 tccttggtat cggcggttcc ggtttcagcg taaaatgtac gcataatgac atgaaattgc
    17941 aagcgtaaaa agcgcccctg gccacggcgg tacggcgaaa gcgcgcccca ccgcatgaaa
    18001 gaggggagga gaggccggca attttgcagc ttgactaaca agaaataatt ctctatcttt
    18061 ttatgagttg taattcctcc cgacgaacct taggggcggg cgctgtgatg aggagcatcc
    18121 cctgcctttt cgacgggtct caattgtatg ctgcgccagg attggaggaa cggatgagcg
    18181 tcattcaact ctcatgggtg gattggtcca ttatcgccct ttatttcgtc ttcatcatcg
    18241 gcatcggctt ctatctcaag cgcttcaccg gcaccggcga agactatttt ctggccggtc
    18301 gcaagaacag ctcctgggtg gcaggactcg ccttcctgag cgccaacctc ggcgccctcg
    18361 agatcctcgg ctggaccggc ggcaccatga aatatggcat gttcgtctcc cattggtact
    18421 ggatcggcgc catccccgcc atgctctttc tcggcctcta catgatgccc ttttactaca
    18481 gcagcaagat ccactccgtg cccggctatc tcaagctccg ttttgatgaa cgctcccgtc
    18541 tgctcaacgc cttctccttt gccatcatga ccctgctcat gtccggcatc aacctctatt
    18601 tgatggccct ggtgctgcgc accatcctcg gttgggattg gcatctctcg atgtggattt
    18661 cagctctcgc cgtcggctgc tacatcat
//
LOCUS       DAOPWU010000024        18425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3061_length_18425_cov_5.006913, whole genome shotgun sequence.
ACCESSION   DAOPWU010000024 DAOPWU010000000
VERSION     DAOPWU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3061_length_18425_cov_5.006913"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PKM23_02705"
     CDS             complement(<1..693)
                     /locus_tag="PKM23_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNY90413.1"
                     /translation="MKACSLIDKARCNCAVVGVYGTTEASRLAYLALYAQQHRGQESS
                     GIVASDGRRVNRHVGRGLVADVFSDQALFATLGGHMAIGHNRYSTTGASHLVNAQPLV
                     ANFREGPFAVSHNGNFTNAGELRRRLEEQGSLFQTTTDTEVVLHLIARSRAQDMTARI
                     AEALLQIEGAYSLVMMCNGRIYAARDPHGVRPLCLGHKEGSWFVASETCALDLLRAEI
                     VRDIEPGELIEIH"
     gene            complement(711..1133)
                     /locus_tag="PKM23_02710"
     CDS             complement(711..1133)
                     /locus_tag="PKM23_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90414.1"
                     /translation="MLAAAGAFAAGSPPEAGSSGGSASAIFYHLPTLLCTAGDTLPAP
                     LPIRRPRGDLWLAKDKADHLVVSAFLVGLGYYAARKEFHLSEPGSTNLGAGFSLSLGL
                     LKEVRDQGRRGGFFSLKDLAADLAGIGLGYLLCSAGAL"
     gene            complement(1162..1302)
                     /locus_tag="PKM23_02715"
     CDS             complement(1162..1302)
                     /locus_tag="PKM23_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90415.1"
                     /translation="MNREEALTLAQSRFRNRNFFVHVLAVEAVMSALTCFFGADDERS
                     GH"
     gene            complement(1420..1944)
                     /locus_tag="PKM23_02720"
     CDS             complement(1420..1944)
                     /locus_tag="PKM23_02720"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNY90416.1"
                     /translation="MKPSAHSLYLPLLGAALLLAAPGLQAQSGAPADSLATRLKSTAA
                     EVRSAPLRKSPTGAMLRSMALPGWGQWYNEKRLKAVVVMGAEAGLVIDAIVQNQLAAR
                     SEADYEREYYRNNRSLAIWWLGAVILYSMADAYVDAHLFDFDERPDLSLAPSSAARTG
                     GSGPALAFNLALHF"
     gene            complement(1954..2346)
                     /locus_tag="PKM23_02725"
     CDS             complement(1954..2346)
                     /locus_tag="PKM23_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNY90417.1"
                     /translation="MRKIIETENAPQAIGPYSQAVVVNGLLFSAGQLGLDPYNGIMVE
                     GGVKAQTLQAMLNLKAILEEAGSALDLVVKTTIFLLDMNDFVTVNGVYAGFFPEHPPA
                     RSTVQVARLPKNALVEIEAVALVPGHQE"
     gene            complement(2351..3652)
                     /locus_tag="PKM23_02730"
     CDS             complement(2351..3652)
                     /locus_tag="PKM23_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNY90418.1"
                     /translation="MTDPETLHMQQREHERLKMLLKVAGSISSEMQLDKLLRLIMDEV
                     KQVLQCDRCTVFVLDREHNELWSRVAHGESEIRFPAHLGIAGAVVQSGQVINIPDAYA
                     DSRFNPNIDRQTGYHTRNLLSAPMRNKPGEIIGVFQILNKLEGPFTREDEEILNAISV
                     LAATQIENAQLYEEQKKTFDSLVETLASTIDARDPLTAGHSQRISLYADEIGRVLRLQ
                     TQEREVLRISALLHDYGKIAVREAVLTKNDKLTDEEFSHIRNHAAYTRSILEKINFSR
                     ELRQVPLIAASHHERVDGTGYPAGLMDDEIPLLSKILAVADVFDALTSKRHYRTRMPF
                     TEVIQLLLDNIGSQFDPLPVNAFRAVKLDRLVRILEHEHPDRLEAEELHFLSGFDSVD
                     YITAAQAEAPEFQPRLVRIFQRYYMQEYLQSSTTAQTQGKG"
     gene            complement(3645..4805)
                     /locus_tag="PKM23_02735"
     CDS             complement(3645..4805)
                     /locus_tag="PKM23_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNY90419.1"
                     /translation="MSDRRLVDRHLVEVIRWSLYIFAAAMPFAIALTQIAVGIALLAW
                     LGRLVYTRAAKWPRLGVEWGFIAFIAAELLACVFSTNLGQNVIYLKRLLLIPIVYLVA
                     ANSSDREELRRLGMIFVVSILLYSVWGIVSYFLNPAVRVRHIQNSMTAGGITMVGAVT
                     AAAFAVRLREMRSRLFMLLAGAVILGCLILTSTRGSWVGFFAAALLILLVQNRRLVWA
                     LPLLVLAFYLLQPGDFSWRMRHIFDPAWGTNAKRIHWWSVGWEIFKDHPLVGIGDVGT
                     TSMYARYAPAGEKELVGHFHSNYVHIGVTLGAIGLAAFLFMTALLLVRLAQSLRARMG
                     ARDWGGGWTLAALAVFAAFSLNGFFEWNFGDQEIITMVWFAIGMGLGQLHHD"
     gene            complement(4802..6703)
                     /locus_tag="PKM23_02740"
     CDS             complement(4802..6703)
                     /locus_tag="PKM23_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNY90420.1"
                     /translation="MALYFRILRYLKPYWTSLLGSLICIFFFTLFSSASLVSIIPFLG
                     TIFGSQQGVEMRAEAPAPGPGAELDEAAPADDGAAGTVRSLRGQLSHYKNVAMAWLTG
                     GSRREALRRLCIFIILLIFLKGFFDYFQSYLMAAVEQGVIRDIRNDLYRQINRLSLGY
                     FTRTRTGQIISRITNDVTLVNSGVSASFVTLIKNPLLIIAYLAIAIYLSWSLTLIALV
                     VAPFSMGIIGWIGLKLKKESTISQERMANLTSLLQETVAGQRVVKAFAMEEFEVRKFG
                     AEAHRYYETLLRITRTRNLASPLTEFLGTMIAVGILWFGGQQVLAGRMLSPEEFMGFL
                     VIVLSLMQPVKELSSVHNRIQEAMAAAERIFEVLDLEPAVQSRPGAIKLTEFREAIEL
                     RGVSFSYGREAEVLRDLSLTIPKGNILAIVGPSGAGKSTLVDLIPRFYDPTEGAIFLD
                     GVDLREIEIGHLRRLMGIVTQETILFNDTVRNNIAYGLSSKPLEEIVEAARIANAHDF
                     ISQLPEGYDTNIGERGIALSGGQRQRLAIARAVLKNPPILILDEATSALDTESELLVQ
                     QAIERLMRNRTSFVIAHRLSTILRADRIIVLEKGRMVQQGVHDELVAQPGLYQKLYTM
                     QFRAAEVQP"
     gene            complement(6774..6962)
                     /locus_tag="PKM23_02745"
     CDS             complement(6774..6962)
                     /locus_tag="PKM23_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90421.1"
                     /translation="MLFRLLILALLFYLATRVFSRLFSSRIDHQETQVHGPSQPNALD
                     LSDEDVEDVDYKELPRNK"
     gene            complement(7000..7635)
                     /locus_tag="PKM23_02750"
     CDS             complement(7000..7635)
                     /locus_tag="PKM23_02750"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNY90422.1"
                     /translation="MNLPRYICIEGVIGAGKTSLTRMLCERLDARAIYEKPDENPFLA
                     DFYRDPQRYAFQVQLFFLLSRYRQQQESLQPDLFHDIAISDYLFAKDRIFAHLNLEDR
                     ELFLYDKIASLLEQDIVKPDLVVYLQSSVERLMANIRKRSRAYEKEMSEEYISSLNES
                     YNRFFFHYQETPLLVVNSVGIDFVNNKADFEELVRQIMRPHSGIEYFSPAR"
     gene            complement(7632..8180)
                     /gene="folK"
                     /locus_tag="PKM23_02755"
     CDS             complement(7632..8180)
                     /gene="folK"
                     /locus_tag="PKM23_02755"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNY90423.1"
                     /translation="MKLNPPRRAPEAFLSLGANLGDREENLIRALQALRGRGIRLGRL
                     SGLYETEPVGREEQPDFLNMVVRLTTLLPPLQLLTRMQEIEQQLGRVRHERWGARTID
                     LDLLCMGGLIWRDERLVLPHPRLHERRFVLVPFAEIAADYEVPGTGATVKELLRDCRD
                     PHRVRLCWSAEQVQLRMRKARA"
     gene            complement(8149..8541)
                     /gene="folB"
                     /locus_tag="PKM23_02760"
     CDS             complement(8149..8541)
                     /gene="folB"
                     /locus_tag="PKM23_02760"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNY90424.1"
                     /translation="MAQDYLRLLGMAFHAYHGLQQEEIENGQRFEVDVELHFDAAPAG
                     RSDRISDTIDVREIYALVRRVVMERRFFLIEAVAEHIAAEILERFPAGGVLVRVRKPF
                     APLGGLANGSEIEIIRHRREAESSPPRP"
     gene            8795..16333
                     /locus_tag="PKM23_02765"
     CDS             8795..16333
                     /locus_tag="PKM23_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNY90425.1"
                     /translation="MSNSALHRFLLPLLLIGSFAILTSPPPAEAARREPWEDQPSPRQ
                     EPLIRYQPVEEAAPLPPARVSAAEADAGALAARSAALWAEGGHARTFLLGGKKLIGRA
                     TIHLDALPPGENRTLLLQVKREREPFHTVASVEAAGSGPVTLTFPGEEATALRVLISG
                     DPEESNPLTSLQAEQIVLAADKRIMLLGDSITAGKYADDNLGYRKVLYDQLRAGGFNV
                     DFVGAYGDSPYEGHFQGGRKTFDFYPDNGFRTPILDVTADMNNYRPHIVGILLGTNNL
                     NDLGDDPVGPCGTAPAFNSTPAGQMAKLIAYLLRWNNNTYGADLQDIFLFLLPPIKGA
                     DSLVAHYNIELARLARDYAGGAITGKAEPVHIVDLYTPFYEDPVFNNNKSTYYPPYLS
                     TPLDPANRLHPNNAGHQLIGQTCYQAFRSQLSGTPPWFTDISWETNTAGYDAEYGGQG
                     IAVADADGDGRDDLYTTRTVTEGAFRRDFFLRQDSEGLFAESSEGWGVRDDGDSRGAL
                     FVDIDSDGDLDLFNGNSPGRNRLYENLNRSSFRDITSTAGIAEIAATTTALLAFDADG
                     DEDIDLYAVNSRTLNELYLNQGNGRFQRADRGANDAEEPGVASESATAADFDQDGDVD
                     IYIAKNGGANRLYLNNGAGWFSDGAAAAGLALNLNCKSAHWADLDNDADLDLIVAVAN
                     TSDPGNLLRLFRNDNGVFTEVSTTANIPMNGFTALTGDFDNDGDLDIITTSDRTTGGF
                     YRNGGGWRFSLVNATGAEVRAGDVRSGVALDYDRDGDLDFFLTRSDIFNLFRENTLDN
                     GNHYLLVDPAGPGASASAFGTRIWCYQAGRAGDPAALLGYREVLSGSGHLAQSPLVQH
                     FGLAGRTACDLIVQFIDNTFLVLGGVPADQTIRVAPLAPTVNPGAAARLIAHAGNNQS
                     GTVGSPLPVPLTVKAVDGAGAPVAGLHVDFSLTAGDAALLLPAGAGSTNLWLEAESGA
                     PGGSLAWAYDASSSGNGLVFVPPSKRTNGRDTLAFSLAEGAASTLWIRARTGTAGSIL
                     SAVMNGGSPYTQSLQAGSDWAWVRLGSPGGWNLAAGTHRLVLEIAPGSLQIDRILVSA
                     SSSYIPSGAGESGSDPFATDSQGLARRTLQLGTTAGPVEVSAQAIEGGRTLTASFRAT
                     AQAGAAASIMIADGNNQVASQGGAPLPSPLIVAVSDGFGNPVQGEMVLFSVRAGGGSL
                     VPADGRVATDSDGRAQAVYITGTLAGQQTIAASPLSLSSKEVLFSATVANGAANLVYL
                     QGDAQADTVRALLAQPVRFKVTRADGQPAASIQITVTAFDGGRVGKTAAVGADSILQL
                     VTGSDGVVSCYWRLGPVSGDQHLKVEAPGLQGSPRVLRAAASPGSPYRILAIDGDGQN
                     GAVKTKLDNPLTARVADRYGNPVPEHPVQFRLLAGGGFLNTSGSDQYSTATNASGAAS
                     VELMLGEVAGINVSRVEAVSQYHSHPLTGSPVIFYASATAGPPHFFYILSGNQQSRVA
                     GSLLPDSLAVMVTDYYQNPLIGHTVTFRVTGGDGKINGLTQAAVATDGGGRAWAWFRL
                     GTKAGPGLQQVTAAAQGLASETLLFTATATADIAARMSYHSGRGQTGLAGSRLADPLV
                     VFVQDRHGNPVAGHQVQFSVTAGGGSFIGEPNINLTSDEEGLAKAWLTLGPAVGDSVH
                     RVSATSLVRSSTTPLIGSPVLFSAGGATQQAGDIKKIEVFAGEGQSAVVDQWLAGALS
                     ARVIDTNNLPVKNHPVVFRIARGNGRLGSGQDTLVSLPTNSSGIAEVIWRLGTLAGDS
                     TQAVEMICLGRSGLPVPGSHRLIYAAARAGAPDINRSTLSVESPVSADGLSASPIITT
                     LRDSYGNPVAGQGILLVGSGLEAELNPNQGYSDGGGLFRSAAVSSLPGSFSVQARVAT
                     SSQWLTEAKTISFIAPPAARLEVAGGDGQTAMVTNWLREPLAVRVLDGIGSALQDREV
                     RFSLVEGSAEIAEPGSARLTALSSPSAAAAVLTVRSDGDGVAAIDVRMGTRSGRVVIF
                     ASLVDDPESRFRFQLRALPGEAAELIRISGDGQSGTTWHRLSQPLIVQVRDAFANPVP
                     AQQVLFSTGQEQGWFQPNAMQTTDSTGAASVLWYLGGVEGTQSAEASVAGAANSPLRF
                     EAATLPNQPPQISLADSVQIRENEKWSYQLEINDLEGDSVRINLKSRPEGLEMSGEGR
                     IDWQPSYQQAGRYELPVEARDQFGAASSARMLLVVVDVNRPPLIDGAACLPQQQEGLQ
                     LTKPDVIDFFVSASDPDGDALSYSWYLNGAFCAYGKPTYRLQSELVSSGDAQVKVVVS
                     DRSTSVSHTWTLKLVSAVWLAGFQAQAEPGRGITLSWRTLLESDHLGFFVQRASREEG
                     PFQTISSLLAPAAEGSYRFTDESAEAGRLYYYRLQDLSRDGSARTHPALQARLPLPLR
                     FNLSANYPNPFNAGTRLTVALPGPGTVRAEVIDLMGRSVRRLHDGETEGGYLTLTWDG
                     RDELGLLAASGVYYCRVSAGNETASRKLVLVK"
     gene            16432..16977
                     /locus_tag="PKM23_02770"
     CDS             16432..16977
                     /locus_tag="PKM23_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNY90426.1"
                     /translation="MVHPWHDVTPGLESPYIFNAITEIPYGSSIKYELDKPSGLLKLD
                     RILYSAVFYPANYGFIPQTLAEDDDPLDVLVFCQEVVQPLTIIKARCIGLMTMVDSGK
                     KDHKIIAVATEDPTYNTFHETTEMPPHTLQMLRRFFEDYKQLEGKVVAVDDILPAKEG
                     YDIVQDALERYSHKRRGGYKF"
     gene            complement(16965..18155)
                     /locus_tag="PKM23_02775"
     CDS             complement(16965..18155)
                     /locus_tag="PKM23_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90427.1"
                     /translation="MLKCRYFPLARRVLFAAALSGSLPASGQALNGGAGLPHTRPAWV
                     LDNGQLTSQMGARFWGQAAAQSYAPFGAPGKRNIWDLQSALAVNCGLGAHLEAQITPV
                     LYQSDQSGGSTFGHDLSATLAVGSLQWPAPSWFLGAAMTAHFPAGARHNLLFEPYSPG
                     RHAVTLTGLASYAVDPEFPAEALSGHVNVGYTLFDDTGLQFHDPITAARSFVVQRSQK
                     FSWSAGVALPGAHFDLGIEGYGQHWLRQPAAGAEGRENFSYASLSLRYKPLRWCHLSL
                     ALDRRLSADADETIPAMAERGLGELPNYPSWLLNLGLHLKLLPLYIMQTSDEEVIMQR
                     VEERTPVFEKIIGEESSKEANRDDLDRLRAEKERAEKELERLRRVIETRQQEQKNPPP
                     DQNL"
     gene            complement(18185..>18425)
                     /locus_tag="PKM23_02780"
     CDS             complement(18185..>18425)
                     /locus_tag="PKM23_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90428.1"
                     /translation="MRPAFYLLRLHEESSGTPVWIGRMAPGYQGTRESTPYNSDGRAA
                     LPRLQSGVLYRWRLDCVGAAARTGSESAWQRFTLY"
BASE COUNT         3480 a         6071 c         5684 g         3190 t
ORIGIN      
        1 atggatctcg atcagctcgc cgggctcgat atcgcgtaca atttcggcgc ggagaaggtc
       61 gagggcgcag gtctcggagg ccacaaacca gctcccctcc ttgtgtccca gacagagcgg
      121 ccgcaccccg tggggatcgc gggcggcata gatgcggccg ttgcacatca tcaccaggga
      181 ataggctccc tcgatctgca gcagggcctc tgctatcctt gcggtcatat cttgcgcgcg
      241 ggaacgggcg atcaggtgca gcaccacctc ggtatcggtg gtggtctgaa agagcgagcc
      301 ctgctcttcg agccggcggc gcagttcgcc cgcattggtg aaattgccgt tatggctgac
      361 ggcgaacggc ccttcgcgaa aattggccac cagcggctgg gcgttgacga gatgggaagc
      421 cccggtggtg gagtaacggt tgtgcccgat ggccatgtgg ccccccagtg tggcaaagag
      481 ggcctggtcg ctgaagacgt cggcgacgag cccgcggccg acgtggcggt tgacgcggcg
      541 gccgtcactg gcgacgatgc cgctgctctc ctgcccgcgg tgctgctggg catagagggc
      601 cagataagcc agacgggagg cctccgtcgt gccgtagacg ccaaccaccg cgcagttgca
      661 gcgcgccttg tcgattaggg aacatgcctt cacttactgc ctcattccag ttagagagcc
      721 ccggccgaac agaggagata acccaagccg atccccgcca gatcggccgc gagatccttg
      781 agactgaaaa agcccccccg ccggccctga tcacgcacct ccttgaggag gcccagagag
      841 aggctgaacc ccgccccgag gttggtactg ccgggttcgg agaggtggaa ctccttgcgc
      901 gccgcatagt aacccaggcc gaccagaaag gcgctcacca ccaggtgatc ggccttgtcc
      961 ttggccagcc agaggtcgcc ccgcggccgc cgtatcggga ggggtgccgg cagggtatcc
     1021 ccggccgtgc acagcagggt tggcaagtga taaaaaatgg cggaggcaga cccgccgctg
     1081 ctcccggcct ccggagggct ccccgctgcg aacgcgccag cagcagccag catgatcata
     1141 agtaacaccc gcttcatcat gctaatgccc cgagcgctca tcatctgcgc cgaaaaaaca
     1201 cgtcagggcg ctcatcaccg cctccacggc cagaacgtgt acgaaaaagt tgcggttccg
     1261 gaagcgcgac tgcgccaggg ttaaagcctc ttctctattc atcaagatta caaccgtttt
     1321 ttgttttttt tccgtcatgc aatggagtta atatacgcat aatctgcctt gattgcaaga
     1381 gacgctatgg cctcctgccg ggagagacag gggggcgggc taaaaatgca gggcgagatt
     1441 gaaggcgagg gcgggtcccg atccgcctgt ccgggcagcg ctggaggggg ccaggctgag
     1501 atccggccgc tcatcgaaat cgaaaagatg cgcatcgacg taggcgtcgg ccatgctgta
     1561 gagaatgacg gcccccagcc accagatggc caggctgcga ttgttgcgat aatactcccg
     1621 ctcgtaatcc gcttccgagc gggcggcgag ctgattctgc acaatggcat cgatgaccag
     1681 ccctgcctcc gcgcccatga caaccacggc cttgagccgc ttttcattat accactgccc
     1741 ccagccgggc aaggccatgg agcggagcat ggcgccggtc ggagacttgc gcaggggggc
     1801 ggaacggacc tcggcggctg tgctctttag ccgggtggcc aggctgtcgg ccggcgcacc
     1861 ggattgggcc tgcaggccgg gcgcagccag gagcagggcc gcgcccagta aaggcaggta
     1921 aaggctgtgg gccgatggtt tcacctcgcc gccctattcc tgatggcccg ggacgagcgc
     1981 caccgcctcg atctccacca gggcgttctt gggcagacgc gccacctgca ccgtcgatcg
     2041 cgcgggcgga tgctcgggaa aaaagccggc gtagaccccg ttcaccgtca caaaatcgtt
     2101 catatcgagc agaaagatcg tcgtcttgac gaccagatcc agcgcggaac ccgcctcctc
     2161 caggatcgcc ttgagattga gcatggcctg cagggtctgg gccttgacgc ccccctcgac
     2221 catgatgccg ttgtagggat ccagtccgag ctggccggcg ctgaaaagca gaccattcac
     2281 caccacagcc tgactgtaag gcccgatcgc ttggggagca ttctccgtct cgatgatttt
     2341 tcgcatgtgt ttatcctttc ccttgcgtct gagcggttgt gctgctctgc aaatattcct
     2401 gcatgtagta acgctgaaaa atccgcacca gccggggctg gaactctggc gcctccgcct
     2461 gcgccgccgt gatgtaatcg acgctgtcaa agccgctgag aaagtggagt tcctccgcct
     2521 ccagccggtc ggggtgttca tgctccagaa tgcgcaccag ccggtccagc ttgaccgccc
     2581 ggaaggcgtt caccggcagg ggatcgaact ggctgccgat attgtcgagc aggagctgga
     2641 tgacctctgt gaagggcatg cgggtgcggt aatggcgttt cgaagtgagg gcatcaaaga
     2701 catcggccac cgccaggatc ttgctcagca gggggatctc atcgtccatc agtccggcgg
     2761 gatagccggt gccgtcgacg cgctcatgat gcgaggcggc gatcaggggc acctggcgca
     2821 gctcccggct gaagttgatc ttctccagga tgctcctggt ataggcggcg tgattgcgga
     2881 tgtgactgaa ctcctcgtcg gtgagcttgt cgttcttggt gagcacagcc tcgcgcacgg
     2941 cgatcttgcc ataatcatgg agcagggccg agatgcgcag cacctcgcgc tcctgggtct
     3001 gcaggcgcag gacgcggccg atctcgtcgg cgtagaggct gatgcgctgg gaatgaccgg
     3061 ccgtgagggg gtcgcgcgca tcgatggtgc tggcgagggt ttccaccagg ctgtcaaagg
     3121 tctttttctg ctcctcatag agttgggcgt tttcgatctg ggttgcggcc aggaccgaaa
     3181 tcgcattgag tatctcctcg tcctcccggg tgaagggacc ctccagcttg ttgaggatct
     3241 ggaacacccc gatgatctca cccggcttgt tgcgcatggg cgcggagagc aggttgcggg
     3301 tgtggtagcc cgtctgcctg tcgatattcg gattgaagcg gctgtcggca taagcatcgg
     3361 ggatgttgat gacctggccg ctctgcacga ccgccccggc gatgccgagg tgggcgggaa
     3421 agcggatctc cgattcacca tgggcgaccc gggaccagag ttcgttatgt tcgcggtcga
     3481 gaacaaagac cgtgcagcga tcgcactgca gcacctgttt gacctcgtcc ataatcagtc
     3541 gcaggagttt gtcaagctgc atctccgagg agatcgagcc ggcgaccttg agcagcatct
     3601 tgagtctttc atgctcgcgc tgctgcatat gcagggtttc ggggtcagtc atgatgcagc
     3661 tgccccaacc ccatcccgat ggcgaaccag accatggtga tgatctcctg gtcgccgaaa
     3721 ttccattcaa aaaaaccgtt gaggctgaag gcggcaaaga ccgccagcgc cgccagcgtc
     3781 cagccgccgc cccagtcgcg cgcccccatc cgcgcccgca ggctttgtgc gaggcgtacc
     3841 agcaggagcg ccgtcatgaa cagaaaggcg gccagcccga tggcgccgag ggtcacgccg
     3901 atgtggacat aattgctgtg gaaatggccg accagttcct tttcgccggc gggggcgtag
     3961 cgggcgtaca tcgacgtcgt gccgacatct ccgatcccca ccaggggatg gtccttgaag
     4021 atctcccagc cgaccgacca ccagtgaatc cgcttggcat tggtccccca ggctggatcg
     4081 aagatgtggc gcatccgcca ggagaaatcc cccggctgca gcagataaaa ggcgaggacg
     4141 agcaggggca gggcccagac cagccggcgg ttctgcacca gcaggatgag cagggcggcc
     4201 gcaaaaaaac ccacccacga gccccgggtt gaggtcaaaa taaggcagcc gagaatgact
     4261 gccccggcca ggagcatgaa caaccggctc cgcatctccc gcagccgcac cgcaaaggcg
     4321 gcggccgtta ccgcgcccac catggtgatg ccacccgccg tcatcgaatt ctggatgtgc
     4381 cgcacccgca cggcgggatt gagaaagtag gagacgatcc cccagaccga ataaagcagg
     4441 atggagacga caaagatcat cccgagacgc ctgagttcct ccctgtcgct gctgttggcc
     4501 gcgaccagat agacgatggg gatgagaagc agccgtttca ggtagatcac gttctggcca
     4561 agattggtcg aaaagacgca ggcgaggagc tcggcggcga tgaaagcgat gaagccccac
     4621 tccacaccta ggcgcggcca ttttgctgcg cgtgtgtaaa ccaggcggcc cagccaggcc
     4681 aggagagcaa tgccgacggc gatctgggtg agggcaatgg caaagggcat ggccgccgcg
     4741 aaaatataga ggctccagcg gatcacctcc acgagatggc ggtcgacaag tctgcggtcg
     4801 ctcacggctg cacctccgca gcacggaact gcatggtgta gagtttctgg tagagcccgg
     4861 gctgcgcgac cagctcgtcg tgcaccccct gctgcaccat ccgccccttc tccaggacga
     4921 tgatgcggtc ggctcggagg atggtcgaaa gccggtgggc gatgacgaaa gaagtgcggt
     4981 tgcgcatcag ccgttcgatg gcctgctgca ccagcagctc cgattcggta tccagggcgg
     5041 aggtggcctc atcaaggatg agaatggggg ggtttttaag caccgccctg gcgatggcca
     5101 gccgctgccg ctgcccgccg gagagggcaa tcccgcgctc gccgatgttg gtgtcgtagc
     5161 cttcgggcag ctggctgatg aaatcgtggg cattggcgat gcgcgccgcc tcgacgatct
     5221 cctcgagggg cttgctgctc aggccgtagg ctatattgtt gcgcacggtg tcattgaaaa
     5281 gaatggtctc ctgggtgacg atgcccatga gccggcgcag atggccgatt tcgatttcgc
     5341 ggaggtccac gccgtccaga aagatcgccc cctccgtggg gtcataaaag cgcgggatga
     5401 ggtcgaccag ggtcgatttt cccgccccgc ttggtccgac aatggcgagg atattgccct
     5461 tggggatggt cagcgagaga tcgcgcagca cctccgcttc gcgtccgtag gaaaaactga
     5521 cgccgcgcag ctcaatcgcc tccctgaact ccgtgagctt gatcgccccg ggccggctct
     5581 gcacggcggg ttcgaggtcg aggacctcga agatgcgctc cgcggccgcc atcgcctcct
     5641 ggatgcggtt atgcacgctc gaaagctcct tcacgggctg catcagcgat aaaacgatga
     5701 ccagaaaacc catgaactcc tcgggggaga gcatccggcc cgccagcacc tgctgcccgc
     5761 caaaccagag gatcccgacg gcgatcatgg tgccgagaaa ctcggtcagg ggcgaggcca
     5821 gattgcgcgt gcgggtgatg cgcagcaagg tctcgtagta gcgatgcgcc tcggcgccga
     5881 atttgcgcac ctcaaactcc tccatggcga aggccttgac cacgcgctgg ccggcgacgg
     5941 tttcctgcaa cagcgaggtg agattggcca tccgctcctg cgagatggtg ctctcctttt
     6001 tcagcttgag gccgatccag ccgatgatgc ccatgctgaa gggggccacc accagggcga
     6061 tgagggtgag cgaccagctc agataaatgg cgatggccag gtaggcgatg atgaggaggg
     6121 gattcttgat cagggtgaca aagctggccg aaaccccgct gttgacgagg gtgacgtcat
     6181 tggtgatgcg cgagatgatc tggccggtcc gggtgcgggt gaaatagccc agagagaggc
     6241 ggttgatctg ccggtaaagg tcgttgcgga tgtcgcggat gaccccctgc tcgacagcgg
     6301 ccatcagata ggactgaaaa tagtcgaaaa atcccttcaa aaatatgagc aggatgatga
     6361 agatgcagag ccgccgcagg gcttcccggc ggctgccgcc cgtgagccag gccatggcga
     6421 cattcttgta gtggcttaac tgtccacgca gggagcgcac cgttccggcg gccccatcat
     6481 cagccggggc cgcctcgtcc agttccgccc caggccccgg cgccggcgcc tccgcccgca
     6541 tctccacacc ctgttgtgag ccaaaaatag tccccaaaaa gggaatgatc gagaccaggg
     6601 aggcgctgct gaacagggta aagaaaaaga tgcagatcag ggaaccgagc agactggtcc
     6661 aatagggttt gagataacgc aggatgcgga aatagagagc cataagagaa atcagttagg
     6721 agaggggcaa agccggcgca ggcggcgaga ccggtccatg atttaagcgc gcatcacttg
     6781 ttgcgcggga gctctttata atcaacatcc tccacatcct catcggagag atcgagggca
     6841 ttgggctggg aggggccgtg cacttgcgtt tcctgatggt cgatccggct ggaaaaaaga
     6901 cggctgaaga ccctggtggc cagataaaag aggagtgcca gaatgaggag acggaataac
     6961 atgggcatat gccttttatt gtgatgaaga cgggtgctgc tatcttgccg gcgaaaaata
     7021 ttcgatgccg gagtgcggcc gcatgatctg gcggaccagt tcctcaaaat cagccttgtt
     7081 gttgacaaaa tcgatgccga cgctgttcac caccagcagc ggggtctcct ggtaatgaaa
     7141 gaaaaagcgg ttgtaggact cgttcaggga ggagatgtac tcttcggaca tctccttctc
     7201 ataggcgcgg ctgcgcttgc ggatattggc catcagccgt tcgaccgagg actggagata
     7261 gaccaccaga tcgggcttga cgatatcctg ctccagcagt gaggcgatct tgtcataaag
     7321 aaagagttcg cgatcctcga gattaagatg ggcgaagatg cggtccttgg cgaagagata
     7381 gtcggagatg gcgatgtcgt gaaaaaggtc cggctgcagg ctctcctgct gctggcggta
     7441 gcggctcagg aggaaaaaga gctgcacctg gaaggcatag cgctggggat cgcggtagaa
     7501 atcggcgaga aagggatttt cgtccggttt ttcataaatc gcccgggcat ccaggcgctc
     7561 gcagagcatg cgggtcaggc tggtcttgcc agcgccgatg acgccttcta tacagatata
     7621 tctcggtaga ttcacgctcg tgcctttcgc atgcgtaatt gcacctgctc cgcgctccag
     7681 cagaggcgca ccctgtgggg gtccctgcag tcccgcagca gctctttgac tgtggcaccc
     7741 gttccgggga cctcataatc cgccgctatc tcggcgaagg gaaccagtac gaaacgccgt
     7801 tcgtggagcc gcggatgcgg cagaaccagc cgctcatccc gccagatcag tccccccatg
     7861 cacagcaggt ccagatcgat cgtgcgcgcc ccccagcgtt cgtgccggac gcggccaagc
     7921 tgctgctcga tctcctgcat ccgcgtcagc agctgcaggg gcggcagcag tgtggtgagc
     7981 cgcaccacca tattgaggaa atcgggctgc tcctccctgc cgaccggttc ggtctcgtag
     8041 aggccggaaa ggcgcccgag gcggattcct ctcccccgca gtgcctgcag cgcccggatc
     8101 agattctctt cgcgatctcc gaggttggca cccaggctga gaaaggcctc aggggcgcgg
     8161 cggggaggat tcagcttcac ggcgatgacg gatgatctcg atctcgctgc cgttggcgag
     8221 tccgcccagc ggtgcgaaag gcttgcgcac gcggaccagc actcctccgg caggaaaacg
     8281 ctccaggatt tcggcggcga tatgttcagc gaccgcctcg atgagaaaaa aacgcctctc
     8341 catgacgacg cggcggacca gggcataaat ctcacggacg tcgatggtgt cgctgatgcg
     8401 atcactgcgg cctgcgggtg cggcgtcgaa atgcagttca acgtccacct caaagcgctg
     8461 gccgttctcg atctcttcct gctgcagtcc gtgataggca tggaaagcca tgcccagaag
     8521 ccgcaaataa tcctgtgcca taaagccggt cgtcctgata taaattaaac ctaatttatc
     8581 gaaataacac cactttgtca agataattca gggccagtgt accccccgaa atctggcttt
     8641 tacctgcctc cttgccccgc gtaccccaat cctccgccgc aaaatcactg caaaatgccc
     8701 ttcacagcgc aaacaactct acgcattgcg cccgattttt cgtatattta attaaaccat
     8761 aatggcccgg tcccgttcga aggagtcgac ctgcttgagt aattctgcat tgcaccgctt
     8821 tctgctgccc ctgcttctga ttggctcatt cgccatcctc acgagtccgc ccccggcgga
     8881 ggccgcgcgc cgcgagccct gggaagacca gccttcacca cgccaggaac ccctgatccg
     8941 ttatcagccg gtggaggagg ccgctcccct gccccccgcc cgggtcagcg ccgcggaggc
     9001 tgatgcaggc gctctcgccg cccgcagcgc cgccctctgg gcggagggcg gccacgccag
     9061 gaccttcctg ctcggcggca aaaaactgat cggccgggcg accatccatc tcgacgccct
     9121 cccgcccggg gagaaccgca ccctgctcct gcaggtcaag cgcgagcgcg agccctttca
     9181 caccgtcgca tcagtggagg ccgccggctc cggaccggtg accctcactt tccccggaga
     9241 ggaagccacc gccctccgcg tactgatttc cggcgacccg gaagagagca atccgctcac
     9301 ctccctccag gccgaacaga ttgtcctggc cgccgacaag cgcatcatgc tcctcggcga
     9361 ttccatcact gcagggaaat atgccgacga caatctcggc taccgcaagg tactctacga
     9421 ccagctcagg gccgggggct ttaacgtcga tttcgtcggc gcctatggcg actcgcccta
     9481 tgaaggccat ttccagggcg gccgcaagac cttcgacttt tatccggata acggtttccg
     9541 cacccccatc ctcgatgtca ccgcggacat gaacaattac cgcccgcaca tcgtcggcat
     9601 tctgctcggc accaacaatc tcaacgacct cggcgacgat ccggtgggcc cctgcggaac
     9661 ggctcccgcg ttcaattcca cccccgccgg acagatggcc aaacttattg cctatctgct
     9721 caggtggaac aacaacacct acggcgccga tctccaggat atcttcctct ttctgctgcc
     9781 gcccatcaag ggcgccgaca gcctggtggc gcactacaac atcgagctcg cccgcctcgc
     9841 ccgcgactac gccggcggcg ccatcaccgg caaggccgaa ccggtgcata tcgtcgacct
     9901 ctacaccccc ttttacgagg atcccgtttt caacaacaac aagtcgacct attacccgcc
     9961 ctacctctcg acgcctctcg atccggccaa ccgccttcat cccaacaacg ccggccatca
    10021 gctcatcggc cagacctgct atcaggcctt caggagccag ctctccggca caccgccgtg
    10081 gttcaccgac atctcctggg agaccaacac ggccggatat gacgccgagt acggcggcca
    10141 ggggatcgcc gtcgccgacg ccgacggcga tggcagagac gacctataca cgacccgcac
    10201 tgtgaccgag ggcgccttcc gccgcgattt tttccttcgc caggactccg aagggctctt
    10261 tgccgagagc tcggagggct ggggcgtcag ggatgacggc gacagccgcg gcgcactctt
    10321 tgtcgacatc gacagcgacg gcgacctcga tctcttcaac ggcaattcgc ccggccgcaa
    10381 ccgcctctac gagaacctca accgcagcag tttccgtgat atcacctcca ccgccggcat
    10441 cgcggagatc gccgccacca ccaccgccct gctcgctttc gacgccgatg gcgatgagga
    10501 tatcgacctc tacgcggtca actcgcgcac gctcaacgaa ctctatttaa accagggcaa
    10561 cggccgcttc cagcgcgccg accgcggcgc caacgacgcc gaagagccgg gggtggccag
    10621 cgaatcggcc acagcggccg atttcgacca ggacggcgac gtcgatatct atatcgccaa
    10681 gaacggcggc gccaaccggc tctatctcaa caacggcgct ggctggttca gcgacggcgc
    10741 cgccgcggcc ggtctggccc tgaacctgaa ctgcaaaagc gcccactggg ccgacctcga
    10801 caacgacgcc gatctcgacc tcatcgttgc agtggccaac acctccgatc cgggcaatct
    10861 cctgcgcctc ttcaggaatg acaacggcgt tttcaccgag gtcagcacca ccgccaacat
    10921 ccccatgaac ggcttcacag ccctgaccgg ggatttcgac aacgacggcg acctcgatat
    10981 catcaccacc agcgacagaa ccaccggcgg cttttaccgc aacgggggcg gctggcgctt
    11041 cagcctcgtc aacgccaccg gcgccgaggt ccgcgccggc gatgtccgca gcggggttgc
    11101 cctggattat gaccgcgacg gggacctgga ctttttcctc acccgctcgg atatcttcaa
    11161 tctgttccgg gaaaacaccc tcgacaacgg caaccactac ctgctcgtcg atccggcagg
    11221 accgggcgcc agcgccagcg cctttggcac caggatctgg tgctaccagg cgggcagggc
    11281 cggcgatccg gccgccctgc tcggctaccg cgaagtgctc tcgggcagcg gccatctcgc
    11341 ccagtcgccc ctcgtccagc acttcggcct cgctggccgc actgcctgcg acctgatcgt
    11401 ccagttcatc gacaacacct tccttgtgct cggcggtgtg ccggcggacc agaccatccg
    11461 ggtcgccccc ctggccccga ccgtgaatcc gggcgccgca gcgaggctga tcgcccatgc
    11521 gggcaataac cagtccggca cggtcggctc cccccttcct gttcccctca cagtgaaggc
    11581 cgtggacgga gccggcgcgc ccgtggcggg cctccatgtc gatttcagcc tcactgccgg
    11641 cgacgccgcc cttcttctgc ccgcgggggc cggctcgacc aatctctggc ttgaggccga
    11701 atccggcgcc cccggaggca gcctcgcctg ggcctacgac gcctcctctt ccgggaacgg
    11761 cctggtcttc gtgcccccat ccaaacgcac caacggccgc gacaccctcg ccttctccct
    11821 cgccgaggga gcagcctcca ccctctggat ccgcgcccgc accggcaccg ccggttcgat
    11881 cctcagcgcc gtcatgaacg gcggcagccc ctatacccag agccttcagg ccggcagcga
    11941 ctgggcctgg gtcagactgg gaagcccggg aggatggaat ctcgccgccg ggacccaccg
    12001 gctggtcctc gagatagccc cgggcagcct gcagatcgac cgcattctgg tgagcgccag
    12061 ttcctcctac attccttcgg gggcgggcga atccggcagc gatccctttg ccaccgacag
    12121 ccagggactg gcccggcgca ccttgcagct cggcaccacc gccgggccgg tggaggtgtc
    12181 agcgcaggct atagaggggg gcagaaccct caccgcctcc ttccgcgcca ccgcccaggc
    12241 cggcgccgca gcctccatca tgatcgccga cggcaacaac caggtggcca gccagggggg
    12301 cgcgcccctg ccctcccccc tgatcgtcgc tgtcagcgac ggcttcggca acccggtcca
    12361 gggcgaaatg gtcctcttca gcgtccgcgc cggcggcggc tcgcttgtcc cggcagatgg
    12421 ccgtgtcgcc accgattccg acggccgtgc gcaggcagtc tacatcaccg gcacactcgc
    12481 cggccagcag accatcgccg cttctcccct ctcgctgagc agcaaagagg tgctcttctc
    12541 cgcgacagtt gccaacgggg ccgccaacct cgtctatctg cagggagacg cccaggccga
    12601 taccgtgcgc gccctcctcg cccagccggt ccgcttcaag gtcacccggg ccgatggcca
    12661 gcccgcggct tccattcaga taaccgtcac ggccttcgac ggcggccgcg tcggcaagac
    12721 agctgcggtc ggagcggact ctattctcca gctcgtcacc ggcagtgacg gagtcgtctc
    12781 ctgctactgg cggctgggcc cggtcagtgg agatcagcat ctcaaggtcg aggcgccggg
    12841 gctgcagggt tcaccccgcg tgctcagggc cgccgcctct cccggctccc cctaccggat
    12901 tctcgccatc gacggagacg gccagaacgg ggctgtcaag accaaactgg ataaccccct
    12961 caccgctcgc gtggcggacc gctacggcaa tcctgtccct gaacacccgg ttcagttccg
    13021 cctccttgcc gggggcggct tcctcaacac cagcggttcg gaccagtaca gcaccgcgac
    13081 caacgcctcc ggggccgcat cggtggagct gatgctgggc gaggtggcgg ggattaatgt
    13141 cagccgggtc gaggctgtga gccagtacca cagccacccc ctgaccggct cgccggtcat
    13201 cttttacgcc agcgccaccg cgggcccgcc ccactttttc tatatattaa gcggcaatca
    13261 gcagtccaga gtcgccggct cgctcctgcc cgactctttg gccgtcatgg tcaccgatta
    13321 ctaccagaat cccctcatcg gccataccgt caccttcagg gtgactgggg gcgacggaaa
    13381 aatcaacggt ctgacgcagg cggcagttgc taccgatggt ggaggacgcg catgggcctg
    13441 gttccgattg ggtacgaaag ccggcccggg gctccagcag gtgacagcgg cggcccaggg
    13501 gctggcctcc gaaacccttc tcttcactgc gaccgccacg gctgatatcg ccgcccggat
    13561 gagctatcac tccggcaggg gtcagaccgg cctggccggc tcccgcctgg ccgatcccct
    13621 ggtcgtattt gttcaggacc gccacggcaa tccggtcgcc ggccatcagg tacagttttc
    13681 ggtcaccgcg ggtgggggca gctttatcgg cgaaccgaac atcaatctga ccagcgatga
    13741 ggaaggcctg gccaaggcct ggctcaccct cggcccggcg gtaggggatt cggtccatcg
    13801 cgtcagtgcc accagcctgg tccgcagcag caccacaccc ctgatcggca gtcccgtgct
    13861 cttcagcgcc ggcggggcca cccagcaggc cggcgacatt aaaaaaatcg aagtattcgc
    13921 cggggagggc cagagcgccg tggtcgatca gtggctcgcc ggagcgctca gcgcccgcgt
    13981 catcgatacc aacaacctcc cggtgaaaaa ccaccccgtc gttttccgca tcgcacgcgg
    14041 caacggccgc ctcggcagcg gccaggatac cctggtcagc ctgcccacca acagcagcgg
    14101 catcgccgag gtgatctggc gcctcggcac ccttgccgga gacagcaccc aggcagtgga
    14161 gatgatctgc ctcggcaggt ccggcctccc cgtgccgggt tcgcaccgcc tcatctacgc
    14221 agccgccagg gcgggcgccc cggacatcaa ccgctcgacc ctgagcgttg aatcgccggt
    14281 ctccgccgac ggcctcagcg caagccccat catcacgacc ttgagggaca gttacggcaa
    14341 tccggtcgcc ggccagggca ttctgctggt cggcagcggt ctggaggccg agctcaatcc
    14401 caaccagggc tacagcgacg gcggcggcct cttcagatcg gcggccgtct catcgctgcc
    14461 gggcagcttt agtgtgcagg cccgcgtggc caccagcagc cagtggctga cggaggccaa
    14521 aaccatctcc tttatcgccc cgccggcggc ccgtcttgaa gtggccggcg gcgacggcca
    14581 gactgcaatg gtcacaaact ggctgcggga acccctcgcc gtacgggtcc tcgacggtat
    14641 cggcagcgct ctgcaggaca gggaggtgcg cttttccctg gtcgagggca gcgcggagat
    14701 cgcagaaccc ggctccgccc ggctcactgc gctctcttcc ccctccgccg cagcggcggt
    14761 tttgaccgta agaagcgacg gcgacggtgt cgccgccatc gatgtgcgca tgggcacgcg
    14821 cagcggccgg gtggtcatct tcgcctccct ggtcgatgat ccggaaagcc ggttccgctt
    14881 ccagctccgg gctctgccgg gcgaagcggc ggagctgatc cgcatcagcg gcgacgggca
    14941 gagcggcacc acctggcacc ggctgagcca gcccctgatt gtacaggtgc gcgacgcctt
    15001 cgccaatccg gttccggccc agcaggtcct cttttcgacc ggccaggagc agggctggtt
    15061 ccagcccaac gccatgcaga ccaccgactc gaccggcgcc gcatcggtcc tctggtatct
    15121 cggcggcgtc gagggcacac agagcgccga ggcctcggtc gccggcgcag ccaactcccc
    15181 cctccgcttc gaggccgcga ccctgcccaa ccagcctccc cagatcagcc tcgccgacag
    15241 cgtccagatc cgtgaaaacg aaaaatggag ctaccagctc gaaatcaacg acctcgaggg
    15301 cgacagcgtc cgaatcaacc tcaaatcccg gcccgaaggg ctggagatga gcggcgaggg
    15361 acggatcgac tggcagcctt cctaccaaca ggcgggacgt tacgagctgc cggtcgaagc
    15421 ccgggaccag ttcggtgcag cctcctccgc ccgcatgctc ctcgtggtcg tggatgtcaa
    15481 ccgcccgccc ctgatcgacg gcgccgcctg cctcccgcag cagcaggagg ggctgcagtt
    15541 gaccaaaccg gatgtcatcg actttttcgt ctccgcctcc gatcccgacg gcgacgcgct
    15601 cagctacagc tggtatctca acggcgcctt ttgcgcctat ggcaaaccca cctaccgcct
    15661 tcagtccgaa ctcgtttcct ccggcgatgc ccaggtcaag gtggtggtca gtgaccgcag
    15721 cacaagtgtc agccacacct ggacgctcaa gctggtctcg gcggtctggc tggccggctt
    15781 ccaggcccag gccgagcccg gccgcggcat caccctgagc tggcggaccc tgctcgagag
    15841 cgaccacctc ggctttttcg tccagcgcgc cagccgggaa gagggcccct tccagaccat
    15901 cagctcgctg ttggccccgg ccgcggaggg gagctaccgc ttcaccgacg agtcggccga
    15961 ggccggcagg ctttactatt atcgtctgca ggacctcagc cgcgacggca gcgcccgcac
    16021 ccatccggcc ttgcaagccc gtctgcccct gccgctccgc ttcaacctct cggccaacta
    16081 tcccaatccc ttcaacgccg ggacgcggct cacggtcgcg ctgccgggcc cggggacggt
    16141 cagggcggag gtcatcgatc tgatggggag aagcgtccgg cggctccacg acggtgaaac
    16201 cgaaggcggc tatctgaccc tgacctggga cggccgcgac gaactcggcc tgctcgccgc
    16261 cagcggggtc tattattgcc gggtgtcggc agggaatgaa acggcgtctc gcaagctggt
    16321 tttggtcaaa taaggagaga ggggaatggc gaagcggatc ggcgcagcgg gaataggggc
    16381 cgcagacacg gcgttagtat gtaaaagtaa aatccatcct gtgaggtgag catggtccat
    16441 ccctggcatg atgtcacacc gggcctcgaa tcgccctaca tcttcaatgc catcaccgaa
    16501 atcccctacg gctcgagcat caaatacgag ctcgacaagc caagcggact gctcaagctc
    16561 gaccgcatcc tctactcggc ggttttttat ccggccaatt acggcttcat cccccagacc
    16621 ctggccgagg atgacgatcc cctcgatgtg ctggtcttct gccaggaggt ggtccagccg
    16681 ctgaccatca tcaaggcccg ctgcatcggc ctgatgacca tggtcgattc aggcaagaag
    16741 gaccacaaga tcatcgccgt cgcgaccgag gatcccacct acaacacctt tcacgaaacc
    16801 accgagatgc ccccccatac cctgcagatg ctgcgccgct ttttcgagga ttacaagcag
    16861 ctcgagggca aggtggtggc ggtcgatgac atcctccccg caaaagaggg ttatgatatc
    16921 gtccaggacg ccctcgagcg ttacagccac aagcgccgcg gcggctacaa gttctgatcc
    16981 ggcggcggat tcttctgttc ctgctgccgg gtctcgatga cccggcgcag gcgttccagc
    17041 tccttttccg ccctctcctt ttcggcgcgc agccgatcga gatcatcccg gttggcttcc
    17101 ttgctgctct cctccccgat aatcttttcg aataccggcg tgcgctcctc cacgcgctgc
    17161 atgatcacct cctcatcgct ggtctgcatg atgtacagcg gcagcagctt gagatggagt
    17221 ccgaggttca gcagccagga gggatagttg ggcagctccc cgaggccgcg ctccgccatg
    17281 gccgggatgg tctcgtcggc gtcggccgag agccggcggt cgagggcgag ggagagatgg
    17341 caccagcgca ggggcttgta gcggagggaa aggctggcgt aggagaaatt ctcccggcct
    17401 tcggcaccgg ctgcgggttg gcggagccag tgctggccgt agccctcaat ccccaggtca
    17461 aagtgcgccc ccggcagcgc cacgccggcg ctccaggaga acttttggct gcgctggacg
    17521 acgaagcttc tggcggcagt gatggggtcg tgaaactgca aaccggtatc atcaaagagg
    17581 gtatagccaa cattgacgtg gccgctgagg gcctcggccg ggaattccgg atcaaccgca
    17641 tagctcgcca ggccggtgag ggtgaccgca tgtctgcccg ggctgtaggg ctcgaagaga
    17701 agattgtggc gggcgccggc cggaaagtgg gctgtcatgg cggcgccgag aaaccaggaa
    17761 ggggccggcc actgcaaaga tcccaccgcc agggtggccg agaggtcatg gccgaaggtg
    17821 ctgccgccgc tctggtctga ctggtagagc accggggtga tctgggcttc gaggtgggcg
    17881 ccgaggccac agtttacggc cagggcgctc tgcagatccc agatattgcg tttgcctggg
    17941 gcgccgaagg gcgcatagct ctgcgccgcc gcctggcccc agaagcgggc ccccatctgc
    18001 gaggtgagct ggccgttgtc gagcacccag gcgggacggg tgtggggcag tccggcgccg
    18061 ccgttgagag cctggccgga cgccggcagg ctgccggaga gggcggcggc gaacaggacc
    18121 cgccgcgcca gtgggaaata tctgcatttg agcatatcgc aactcccagc tagatcctgg
    18181 tcggtcaata aagggtgaaa cgctgccagg ccgattcgct tccggtccgc gcggccgcgc
    18241 cgacgcaatc gaggcgccaa cggtagagaa ccccgctctg caggcgaggc agagcggccc
    18301 ggccatcgct gttgtaaggg gtgctctcgc gcgtcccctg ataaccgggt gccatccggc
    18361 cgatccatac cggcgttccg ctgctctctt cgtgcaagcg gagcagataa aaggccgggc
    18421 gcatc
//
LOCUS       DAOPWU010000025        18385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3077_length_18385_cov_5.556738, whole genome shotgun sequence.
ACCESSION   DAOPWU010000025 DAOPWU010000000
VERSION     DAOPWU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18385
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3077_length_18385_cov_5.556738"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKM23_02785"
     CDS             complement(<1..257)
                     /locus_tag="PKM23_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNY90429.1"
                     /translation="MEISLQGKVAIITGSIQGIGRATAEMLAANGAAVVLNNHENPTQ
                     LEEVVAGIRAKGGRAQGIIADVTKREEAQKLVDAALALGRI"
     gene            complement(270..2189)
                     /locus_tag="PKM23_02790"
     CDS             complement(270..2189)
                     /locus_tag="PKM23_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90430.1"
                     /translation="MNSFNRGFCRCTGLLSLIVLLNAPLCPAWESQIVQVHTGKLVYR
                     ADEEGNRIPDFSHAGYKGGGVPLPEIPVVKTISPIAGDNTAHIQNALFEVGLLPQNEA
                     GFRGALLLSAGKYEIRGTIKLGFDGVVLRGAGDGEDPATSTILWATGNSPNQRTVLIA
                     GGGGTTRWRDEVANTRRDITSDTVLVGSRRFDLSDASPFAVGDNIIIYHPCTEDWLAA
                     IDYGGTWSQDPGAEPGVDLPWAIGSQNIVYNRRITGVAGSTITIDAPLFDHLIRKQSQ
                     SYIYKYARTGLRSRIGIENLRIDIETRGGVDEAHAWTAIDLFQIEDAWVRECTLLHFG
                     HSGVRTNTATRITIENCRALDPVSTIEGERRYNFNVYNASQLVLFKNCHATNGRHHYV
                     SNGASWTSGCVFLDCTSSGAWTSSEGHRQWSTGLLWDNVVDLDGPRPGYNPRLLGLYN
                     RGHYGTSHGWGVAHSVAWNCDVAAGDLIVQKPPTAQNYAIGCQGKNITGKKPPAPFAE
                     PEGYIEGSSTPGLEPRSLYEAQLAERLAATPVALEGWLSGAKQPASCALAQNYPNPFN
                     GETRLEFALDRPGHITLTIQSVRGEQIAALAAGQFPAGVHSVTWLAGSLPSGLYFARL
                     THEDKTITKRMLLIK"
     gene            complement(2271..4451)
                     /locus_tag="PKM23_02795"
     CDS             complement(2271..4451)
                     /locus_tag="PKM23_02795"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HNY90431.1"
                     /translation="MMRTARTLLLLAAFSLAWCAEADAGRIYRVQNPAEITSAMTTAQ
                     PGDTLLMVRGVWNNTAIVFKGSGQEGRPIVLAAETPGAVVLNGTSTLRISGSWLVVDG
                     LRFVGGYSPSGAVIEFRSGAPAYHSRLTNTSIVDYNPASRSNDYKWVSLYGTHNRVDH
                     CYFAGKNHMGTTLVVWFPTHPHYHLIDLNWFGYRPPLGENGGETIRIGTSDWSMENSR
                     TTVEYNYFEHCNGEIEIISNKSCENVYRYNTFVECEGALTLRHGNRCMVEGNFFLGNR
                     NSEAGGVRVIGEEHTIFNNYFSGLWGSSMKSALPIMNGVPDSPLDRYFQVKKAVVAFN
                     TFVDCRYPFILGSGSDSERTLPPQDCIIANNVVQGGTRLVTQEDAPVNLLWEGNIMHG
                     SSLGIPQPDGILLADPRLFQATDGLWRPDSTSPLRSAATGSYPFITADMDGQVRIAPL
                     DAGADQISAELVQRRPLTPADVMPAWMKNPAPVVLTLLRNGSGRVSVDPAGGVYPPGT
                     RVTVTAVPDPGWRFERWEGDPGGSSNPLMVTLDQDLLLRALFVTDAPLRFKLDVFVFS
                     SGGKVALDPPGGSYPAGTVVRMTALPDSGWSFDRWEGALIGRNNPDSLSMDSDKMVLA
                     VFARASFVDTATKVPVDFSLAQNYPNPFNGRTRIQFTLTSPGPVELKIFNSAGGEAAT
                     LVKGFLTAGTHRIAFEANGLPAGIYFCRLVSGTLTAVNKMVIIN"
     gene            complement(4540..8781)
                     /locus_tag="PKM23_02800"
     CDS             complement(4540..8781)
                     /locus_tag="PKM23_02800"
                     /inference="COORDINATES: protein motif:HMM:NF028470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5123 domain-containing protein"
                     /protein_id="HNY90432.1"
                     /translation="MKKTLYLSLFLLLALLLTAGGAPAQILYQVAAGENTLNAAIEAA
                     ASGDIIELTDSGGLYSYNGSDKMYITKSLTIRAAEGLAEKPIIRNITPAAGSPRIFEI
                     RKGGNLHLKGLNLDGRAEEGGAAFVKNIIRSEDVAAAADSFHFVLKVEDCWLHDSKES
                     LLRGHMFTTADTILFRDCIFRNAYNEAIVLRERTNEAPEVKYLELENCTFAKIGREAL
                     YVEYTNPVVRINHCTFDSVSYRENKRILNPKGVTDVEVKNSIFSNQGGTFSTSVELFG
                     TSTIAYCDTFNVAAVKLNDTAAIGAGMLDADPLYTDAAAWDFTLLPGSPVLAMADDGE
                     AMGDLRWAPRPVGPVVHQVAAGENTLNAAIEAAASGDIIELTDSGGLYSYNGSDKMYI
                     TKTLTIRAAAGLAEKPIIRNITPAAGSPRIFEIRAGGSLHLMGLNLDGRAEEGGAAFV
                     KNIIRSEDVAAAADSFHFTLKVEDCWLHDSKESLLRGHQFTIADTILFRDCIFRNAYN
                     EAIVLRERTNEAPEVKYLELENCTFVKIGREALYVEYTNPVIRINHCTFDSVSYRENK
                     RILYPRDVQDVQIKNCIFSNQGGTASASVEIYGASSLSYSDLFNTKIAKLNGSEITGS
                     NNLAVDPLYYNPAAFDYRLAVNSPVRGAADDGRAMGDLRWEVSPDQRLLTVLTEGKGV
                     VTLDPPGGAYAPGTVVTLTAVPEAGWKLAGWEGVLIFPPDNPVATITMDSDKTVKAKF
                     ENLAPRVAVTVDTLGLGHVVLDPAPVEGKYFQGDLVTLTAVPQADWRFVEWLGDLTGI
                     VNPVQVSIDSAMHITASFASTFTQFALTLTVEGKGSVVADPAPILGTYDSSTVVTLTA
                     TAVQGWEFSGWSGDLTGNENPASVTMDAPRAVTATFAEKAFTRRALEIDASWDLYDAV
                     EFANNNSTIDSLVLITSGGIYTSIHTEDVAVRAPLTIAAAPGLAQKPVVTNSDPEKTN
                     LDVFRVFDDFTLDGVVIDGGHEQSHGMKYAIRLRDYDQIDTVRHGADITLRNCDLINF
                     FELKDPTKDGHAVRFDVNLVAGTVRIENCSFAHFGYEAIRISETEKYPTDRCLDSLII
                     RNCTFTDIDAECVRYYSDLDAATPDAPVIIEHVTVNNSSTAAFYLKNSGGAVVRDIII
                     ANTRTSGHGRDGNLMDCQGNTGVPAYVSHIDTFHVAKVDIKATDGEVDAATVWGIDPL
                     FRDAANQDYTLLAASHLYGLGHDSEALGDLRWATQTPTHVSLHLVIDGPGQVLVDPAP
                     VGKTWDPNTVVTLHAVPDSAHYFEGWAGEINGLVNPVEVTLDQSKIITAMFRLITGID
                     GNGALPEAYALEQNYPNPFNPATTISFALKQPGRTRLLVFDMLGRVVAAPVDRQMAAG
                     RYSVSFQLPELASGVYFYKLESGTFTSIKKMMLVK"
     gene            complement(8819..10477)
                     /locus_tag="PKM23_02805"
     CDS             complement(8819..10477)
                     /locus_tag="PKM23_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90433.1"
                     /translation="MKLGNVMAILLMAALALASAAQASKIEVDTQYDFKLALDYVFTA
                     RVDTILLVTSGGIYTTADTMHLQIKKPVVIMAKPGLAEKPVFRHGDLDSSVIEIFRIH
                     DDVIFQGIIFDGYNAKRPMKYGLRVGHGPATQIPRIFAREGLNITIRDCEFRDFFPPN
                     WENDAGGNAFYFLAPASGEPVIKAGNVKIENCTFRNIGDEAIRIAETEKYKVTRALDS
                     LIVRNCTFKNVFAECVRFYADTDTSSADAYVLIEHLTVDSCGTRMAYFKNNKNVIFRD
                     ILVTNSHLPKPYRAERADYVVQVQLPGSTISHIDTLNLIFAVPYSSRISASKGGKVNG
                     ATIYAFDPLYRNTLAGDYTLLADSPAYGSAHDGQALGDLRWATQTATRVPFLLTIEGM
                     GTVLCDPPLIAPNYPTGTTVTLTAVPDSAWQFAGWSGAITGAANPATVTVNGSTSIAV
                     KFEQSTGVAAAPLQPAVYALAQNCPNPFNASTTLNFSLEREGMTSLVLYNMLGQKLRT
                     LLEKKLTAGAHSLNFDAADLPSGIYLYQLRSGSFQATRKFILMK"
     gene            complement(10489..11964)
                     /locus_tag="PKM23_02810"
     CDS             complement(10489..11964)
                     /locus_tag="PKM23_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90434.1"
                     /translation="MFRKTTLIFLLFAAAAPAQHRGDNLAFQGLPLNQPPGVRALALG
                     AAFTAQSGDPGALWFNPAGLAGIEKPQISLSLSHATPRWRENQVYRPNRMFWTLAFYL
                     EGLYTPDPADNGIWDFELAQDSSYRIDEPALGLEPFSAAAADWQRDLKRSGLQQISAA
                     WPLRISSQKVLVSAGYARSAIDDFDRNDTFLDPHIGYDEYGTVPRVVNDTVRFSWSRF
                     LRQREGDLHTFTAGLAWAPLDRIQLGLSAALLDGQSEDLQYLRRVGWFDIAKDNRFRF
                     AYDTLDVVTRGTSDYRALQYTLGALVKLERFSLGLQVKMPCTISRKWRASTVTTTAGG
                     PEAMATAGTADFRHPVSYAFGAAFQPIDPFRLFLDYQFTPYSQGGIDLTADDPTNRVL
                     PDQSDLRCGISWQPLPFLELLAGFRQTTALFVPDGAAIKDRGPSADTWSLGTAITLAR
                     YGRLELAMASRKMNYYDSYYSNANYVTETFSNLVAGYTYQF"
     gene            complement(12042..13070)
                     /locus_tag="PKM23_02815"
     CDS             complement(12042..13070)
                     /locus_tag="PKM23_02815"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY90435.1"
                     /translation="MNGKITRLLFVFLMTAGPILAFEKVGTTAFQFLKVPASARGCAM
                     GEAFSAAADHSDAAFFNPAALTRIRKMDISFDYMDYFLDISHVAVSGAWSVPNLGTFG
                     LHAIATDIGSIRVTSVEALDFYDGVYLGYTGETIEPGSALIGASFARDLTDKFAFGLS
                     AKYAREDLGVRSAGTLLFDGGLTYDTGFMGLQIAAAIRHFGPEVKFYDQVSLPRYDAS
                     TDSTYYQRYNGKGYPLPQTFNIGMAAWLISASGHSLIHSRNQTLLLAFDLVQPRDYDQ
                     QYNFGLEYGFEGLLFLRAGYKLNYDTEGLSLGFGLAWNRMRIDYAYSDYGAWLDAVHR
                     FTFGFVVN"
     gene            complement(13167..15398)
                     /locus_tag="PKM23_02820"
     CDS             complement(13167..15398)
                     /locus_tag="PKM23_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90436.1"
                     /translation="MKNISLLILAAGFLGLLLLPAGAQELEFMIHSRGMLHETVFNSG
                     DIGRPWTTGDEGNKTTTPLMEWPSRSATMVQGTRYSGQHNIIGAGVYMAANPDGQPGM
                     ESRRYALCGAVGTGAGSETIIGRWSYPLGMERIENFPLLPDGSLNPEYNPDEAEEIIT
                     ASWATPLGVTVTRTSRAWSYPDYDDMIIYEYELEYTGDTDGNPATIEQSAVLKDFMAL
                     FIYGFAPSMYGYQRHYQEWKYESGMYRGDQNNFWDPDYWLSFNMNLRTNISDEALFAK
                     REPNKELFLEFARTGKNGGGLCSPQAPGFCMLYYDTSHLAFVDTIESARNESEAVAVL
                     RTTLGKYYELDENNHIKQPWSNKVSTGNTRSSKMVVDAINPDKRWSGVYSEGSTTWPN
                     PPSARWIGRAAYNYRQSSDAAQKHIVFGPYTLRPGDRLEFSLAEVVGFGAAPGKVVEG
                     GQALVQWSATPSWNRRVTFAGEVVTEHYLDDFGYPDYINSEVITVTDVAHKAFQAYLG
                     ADSIALPVWPEENPRDGDYRIPVPVPAPVIMVENTSIGQVRLTWKDGPDRFTHPRLTG
                     ALREYRIYRSDAGMGPWALLATLPAGQTTGSGLYEYIDADPVFKLGERRYYSVVSVND
                     QGHSSGRTNITRHDKNVASVAKLGEVFVAPNPFNIESGFGGKTGAEDKIGFYGLPERC
                     TIRIFSYAGQLIQTIEHNEPVYTTAWFQVTRNEQEIASGIYLYVITTPGGDRVSGKFI
                     IVK"
     gene            complement(15432..18305)
                     /locus_tag="PKM23_02825"
     CDS             complement(15432..18305)
                     /locus_tag="PKM23_02825"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY90437.1"
                     /translation="MLHRIKAPLGSVALLLICLLSFPLRAGETGKIAGSVKDKQNGQS
                     LPGANVMITAVWQGDRVAALERPTGAVSDTQGQYFILNLPPGYYNVRISYIGYIQQLH
                     EKVQVKIDKTTRLDFALEATALAGEQVVVTAYRPDKVEKDLTATKLTYDVGEVISIAG
                     VESIADILELQADVVDDHFRGGRVGESTYLIGGAAIVNPLSNERAFLPIVTALEQVEV
                     YTSGFSAEYGNAQSGVVNMVAKEGKSSWDSRMEFSSTLPYYKSWGGSPYSPENLDYFH
                     LLYDPEEWLKDNPLLPGHPLWENGYGVAKYLPPRIVWPPNPLNHQDSLRMALIGRNLW
                     LQSVRDVGLESRAMDYRFDFTAGGPVAKNTQMFVAMRQSMISPLVPTPNPDLERQMLG
                     NLTWQPSADDKLKLSVMYDFSFLNYLDSNWERWIFDRTLSVTKKTNSSLQYGLEWRHL
                     FSPASYGDFNFKVLDVRQRDRIELLTDDQYLDDYGNNRNWVDYTAPSNQRVGRPNDDR
                     GSQKTRTWSLTSSYTSQINRYNLLKSGLQFYYYDLDVDYQYNATNPQNLQNLTFRSFP
                     FEGALYLQDKMEFEGLIANIGLRFDFYDLNTRYYLDKFYPLRDPEKTEKTGLFTRLQP
                     RIGISFPVSEYSVFHLNYGTFTQRPNFYQLFYNEINDDGGIVTLYTLGNPELKPENTQ
                     AYDIGIVRSLAGGFQIDVSAYYKDVKNLVESAFYKTEGGESYRTYINRDYADIKGFHV
                     NFERVTGSLRGYLRYNYEEAKGKNSNPDNLAVPVTFFNYTPSEEELADLAEQRFPEDV
                     FLDYDRKHKMVINLRYLTGKESGFEILGFRPLANLSVSNTFRYYTGRPYTLPPSLDPF
                     GQALKFSKRTPDERDWRIRLEKRFGSGRSSLTAYVEGFNMLNQKTWSYSRTFNHGRNT
                     VRWHTDRANILTDDEFAPYVTGQELYLLTNEPRHFRMGMIFHF"
BASE COUNT         3516 a         4977 c         6210 g         3682 t
ORIGIN      
        1 tcgatccttc ccagggcgag ggcggcatcg accagttttt gcgcctcttc ccgtttggtt
       61 acatcggcga ttatgccctg cgcccggccg cctttcgcgc ggatccccgc gacgacctct
      121 tcgagctggg tgggattttc atgattgttg aggaccaccg cggccccatt agccgccagc
      181 atctctgcgg tagcccggcc gatgccctgg atgctgccgg tgatgatggc gaccttgcct
      241 tgcaatgata tctccatgat tcactccttt tatttaatca ataacatacg tttagtaatc
      301 gttttgtcct cgtgtgtcag ccgggcgaaa tagaggcccg atggcaggga tccggccagc
      361 caggtcaccg agtgaactcc cgccgggaac tgccccgcgg ccagagcggc gatctgttca
      421 ccccgcactg attggatggt gagggtgata tgccccggcc tgtccagggc gaattcgagc
      481 ctggtctcgc cgttgaaggg attgggataa ttctgggcga gagcgcagga ggcgggctgt
      541 ttggcgcccg acagccaacc ctcgagcgcc accggggtgg cggccagccg ctccgccagc
      601 tgggcctcat agagtgagcg cggctcgagg ccgggggtgc tgctgccctc gatatagccc
      661 tccggctcgg caaagggggc cggcggcttt ttaccggtga tgttcttgcc ctggcagccg
      721 atggcgtaat tctgcgctgt gggtggtttc tgcacgatca ggtcgccggc agcgacatcg
      781 cagttccagg caacactgtg agccacaccc cagccgtggc tggtgccgta atggccgcga
      841 ttgtagaggc cgagcaggcg gggattgtag ccggggcggg ggccgtcgag gtcgacgacg
      901 ttatcccaga gcagcccggt gctccactgc cggtgccctt cactgctggt ccaggcaccg
      961 gaagaggtgc agtcgagaaa gacacagccc gaggtccagc tggcgccgtt ggagacatag
     1021 tgatggcggc cgttggtggc gtggcagttt ttaaagagca ccagctgtga ggcattgtag
     1081 acattgaaat tataacggcg ttccccctcg atggtggaga ccggatcgag ggcgcggcag
     1141 ttttcaatgg tgatgcgggt ggcggtgttg gtgcgcaccc ccgaatggcc gaaatggagc
     1201 agggtgcact cgcgcaccca ggcgtcctcg atctggaaaa ggtcgatggc tgtccaggca
     1261 tgggcctcgt cgacaccgcc cctggtctcg atatcgatgc gcagattttc aatgccgatg
     1321 cggctgcgca gccctgtgcg cgcatacttg tagatatagg actgcgactg cttgcggatg
     1381 aggtggtcga agagcggggc atcgatggtg atggtgctgc ccgcgacgcc ggtgatgcgg
     1441 cggttgtaga cgatgttctg gctgccgatc gcccagggca gatcgacgcc gggctctgcc
     1501 cccgggtcct gagaccaggt gccgccataa tcgatggccg ccagccagtc ctccgtgcag
     1561 ggatgataga tgatgatatt gtcccccacc gcaaagggcg aggcatcgct gaggtcaaag
     1621 cggcggctgc ccaccagcac ggtgtcggag gtgatgtcgc ggcgggtgtt ggcgacctcg
     1681 tcgcgccagc gcgtcgtccc cccgcccccg gctatgagga ccgtgcgctg gttcgggctg
     1741 ttgccggtcg cccacagaat cgtgctggtg gccggatcct cgccgtcccc ggccccgcgc
     1801 aggacgacgc cgtcgaagcc cagcttgatg gtgccgcgga tttcatattt cccggcgctc
     1861 agcagcaggg cgccgcggaa gcctgcctca ttctgcggta aaagccccac ctcgaaaagg
     1921 gcattctgga tatgcgcggt attgtccccc gcgatcggcg agatggtttt gacgaccgga
     1981 atttccggca ggggtacccc gccgcccttg tagccggcgt gactaaagtc ggggatgcgg
     2041 ttgccctcct cgtccgcgcg atagaccagc ttgcccgtgt ggacctggac gatttgtgat
     2101 tcccaggcag ggcaaagggg tgcattgagc agcacaatga gcgacagcag cccggtgcag
     2161 cgacaaaaac ctctgttaaa tgagttcaag tcagggatac tccgatataa agtatagtct
     2221 ctgcgcgcac atgctgtttt atgcagcgcg gccatccatg cctgaaacag ctagttgatg
     2281 atcaccattt tgttcaccgc agttagggtt ccggaaacca gccggcaaaa atagatgccg
     2341 gccggcagcc cgttagcctc gaacgcgatc cggtgggttc cggcagtgag aaatcctttc
     2401 acgagggtgg ccgcctcgcc accggcgctg ttgaatattt tcagttcgac cggcccgggg
     2461 ctggtaagtg tgaactggat tcgcgtccgt ccattaaagg gattgggata gttctgcgcc
     2521 aggctgaaat ccactggcac tttcgtcgca gtatcgacga agctggcccg ggcgaagacc
     2581 gcaagaacca ttttatcgct gtccatgctg aggctgtcgg gattgttgcg gccgatgagg
     2641 gccccctccc agcggtcaaa gctccagccg ctatctggca gggcggtcat ccggaccacc
     2701 gtccctgcgg gatagcttcc acccggggga tcgagcgcga ccttgccgcc ggaggaaaag
     2761 acaaagacat cgagcttgaa acggagagga gcgtcggtca cgaagagggc gcgcagcaga
     2821 agatcctggt cgagtgtgac catcagggga ttgctgctgc cgcccggatc gccctcccag
     2881 cgctcgaagc gccagccggg atccggcacc gcggttaccg tcactcgcgt accgggcgga
     2941 taaacgcccc ccgcgggatc aacgctcacc cggccggagc cgtttctgag cagggtcagc
     3001 actaccgggg caggattttt catccaggcc ggcatgacat cggcgggggt caagggacgg
     3061 cgctgcacca gctccgccga tatctgatca gccccggcgt ccagaggggc gatgcgcact
     3121 tgtccgtcca tgtccgcagt gatgaaggga tagctgccag ttgcagcact gcgcaggggg
     3181 ctggtgctgt cggggcgcca gaggccgtcg gtggcctgaa agaggcgggg atcggcaagg
     3241 aggatgccat ccggctgggg gatgcccagg gaactcccgt gcatgatatt gccctcccag
     3301 aggaggttaa cgggggcgtc ttcctgcgtg accagcctgg ttccgccctg gacgacatta
     3361 ttggcgatga tgcaatcctg cgggggcagg gtgcgctcgc tgtcgctgcc ggagccgagg
     3421 atgaagggat agcggcagtc gacgaaggta ttgaaagcca cgaccgcctt cttgacctgg
     3481 aagtagcggt cgagggggct gtcgggcacg ccgttcataa tcggcagggc tgatttcatg
     3541 ctgcttcccc aaagcccgga aaagtagttg ttgaagatgg tgtgctcctc accgatcacc
     3601 cgcacgccgc ctgcctcgct gttgcggttg ccgaggaaga aattgccctc caccatgcag
     3661 cggttgccat ggcgcagggt gagcgctccc tcgcattcga cgaaggtatt atatcggtaa
     3721 acgttctcgc agctcttgtt ggagatgatc tcgatctcac cgttgcaatg ttcgaaatag
     3781 ttgtattcga ccgtggtgcg cgagttctcc atggaccagt cgctggtgcc gatgcggatg
     3841 gtctcgccgc cgttctcccc gaggggggga cggtagccga accagttgag gtcgatgagg
     3901 tgataatggg gatgggtggg aaaccagacc accagggtcg tccccatgtg gtttttgccg
     3961 gcgaaatagc agtggtccac ccggttgtgg gttccgtaga gggaaaccca tttgtaatcg
     4021 ttcgatctgg aggcgggatt gtaatcgacg atgctggtgt tggtgaggcg cgaatggtag
     4081 gccggagcac ccgagcggaa ttcgatcacc gcgccggaag ggctgtagcc cccgacgaag
     4141 cgcaggccgt cgacgaccag ccatgagccg gagatgcgca gggtggaggt gccgttgaga
     4201 acgaccgcac ccggcgtctc agccgcgagg acgatcggcc ggccctcctg cccgctgccc
     4261 ttgaaaacaa tggcggtgtt gttccacacc ccccggacca tgagcagggt gtctccgggc
     4321 tgagcggtgg tcatcgcgga tgtgatttcg gccgggttct gaacccggta gatccggccg
     4381 gcatcggcct ccgcgcacca ggcgaggctg aaggcggcca ggagtagtag tgtacgtgca
     4441 gttcgcatca tggcattcac gacagagagg tgatgggaca aacccggcga ggcgcgcaga
     4501 gcgccccgcc gggctgtcgg tgtgaaggga tgggatattt tacttgacca gcatcatctt
     4561 tttgatcgag gtgaatgtac cggactcgag cttgtaaaag tagacccccg aggccagttc
     4621 cggcagctga aaactcacgc tgtagcgccc cgccgccatc tggcggtcca ccggagcggc
     4681 gaccactctt ccaagcatgt caaaaaccag cagcctggtc ctgccgggct gcttgagggc
     4741 aaagctgatg gtggtggcgg gattgaaggg gttggggtag ttctgctcga gggcataggc
     4801 ctcgggcagc gccccgttgc catcgatccc ggtgatgagg cggaacatcg cggtgataat
     4861 cttgctctga tccagggtca cctcaacggg attgaccagg ccgttgattt cgccggccca
     4921 gccttcgaaa tagtgggcgg agtcgggcac cgcatgtagg gtgaccacgg tattgggatc
     4981 ccaggtcttg ccgacagggg cgggatcgac cagcacctgg cctgggccat cgatcacgag
     5041 atggagactg acatgggtcg gggtctgggt cgcccagcgc aaatcgccga gggcctcgct
     5101 atcatggccc aacccataga gatgggaggc ggccagcagg gtataatcct ggttcgcggc
     5161 atccctgaag agcggatcaa tgccccatac cgtcgctgca tcgacttcgc catcggtggc
     5221 cttgatatcg accttggcca cgtggaaggt atcgatgtga gagacgtagg cgggcacgcc
     5281 ggtgttgccc tggcagtcca tgagattgcc gtcacggcca tggccggagg tgcgggtgtt
     5341 ggcgatgatg atgtcgcgga cgaccgcgcc gccgctgttt ttcaggtaaa aggccgcggt
     5401 gctggagttg ttgaccgtga catgctcgat gatcaccggg gcatccggag tggcggcatc
     5461 gaggtcggag tagtagcgca cgcattcggc gtcgatgtca gtgaaggtgc agttgcggat
     5521 gatcagcgaa tcgagacagc ggtcggtggg atacttttcg gtctcggaga tgcggatcgc
     5581 ctcgtaaccg aaatgggcga atgagcagtt ctcgatccgg accgtaccgg cgacgaggtt
     5641 gacatcaaaa cgcacggcgt ggccgtcctt ggtgggatcc ttcagttcga agaaattgat
     5701 cagatcgcag ttgcgcaggg tgatgtcggc gccgtggcgg acagtgtcga tctggtcgta
     5761 atcgcgcagc cggatggcgt atttcatgcc gtggctctgt tcatggccgc cgtcgatgac
     5821 cacaccgtcg agggtgaaat catcaaagac ccggaagaca tcaaggttgg tcttttccgg
     5881 atcgctgttg gtgacaacgg gtttttgcgc aaggccgggc gctgcggcga tggtgagcgg
     5941 agcgcgcacc gccacatcct cggtatggat ggaggtgtag attccgccgc tggtgatgag
     6001 caccaggctg tcgatggtgc tgttgttgtt ggcgaactcg accgcgtcat agagatccca
     6061 gctcgcgtcg atctccagcg cccggcgggt gaaggccttt tcggcaaagg tggcagtcac
     6121 ggccctgggt gcgtccatgg tcacgctggc gggattctca ttcccggtca agtccccgct
     6181 ccagccgctg aactcccatc cctgcacagc cgtagcggtg agggtgacca ccgtactgct
     6241 gtcataggtg ccgagaatgg gggcggggtc cgccacaacc gaccccttgc cctcaacagt
     6301 cagggtgagg gcgaactggg tgaaggtgct ggcgaacgag gcggtgatgt gcatggcgga
     6361 gtcgatcgac acctggaccg gattgactat gccggtcaga tcgcccagcc actcgacaaa
     6421 tctccagtcg gcctggggca ccgccgtcag ggtgaccaga tcgccctgga agtatttgcc
     6481 ttcgaccggt gcgggatcga gcacaacatg gccgaggccg agggtatcga ccgtcaccgc
     6541 gacccgcgga gccaggttct cgaatttggc tttgacggtc ttatcgctgt ccatggtgat
     6601 ggtggcgaca ggattgtccg gcgggaagat gagcacaccc tcccagccag ccagcttcca
     6661 gccggcttcc ggcacagcgg tcagggtcac caccgttccg ggtgcatagg ccccgcccgg
     6721 aggatcgaga gtgaccacac ccttgccctc ggtcagcacg gtgagcaggc gttggtcggg
     6781 agagacctcc cagcgcagat cccccatcgc ccggccgtcg tcggcggcgc cgcggaccgg
     6841 cgagttcact gccagccggt aatcaaaggc cgccggattg taatagaggg gatcgaccgc
     6901 caggttgttg ctgccggtga tctcactgcc gttgagcttg gcgatcttgg tgttgaaaag
     6961 gtcggagtag ctcagcgagg aggcgccgta gatctcgacc gaggccgagg cggtgccgcc
     7021 ctggttggaa aagatgcagt tcttgatctg aacgtcctgg acatccctgg gatagaggat
     7081 gcgcttgttc tcgcggtagg agaccgaatc aaaggtgcag tggttgatcc ggatcaccgg
     7141 attggtgtac tcgacgtaga gggcttcgcg tccgatcttg acgaaggtgc agttctccag
     7201 ttcgagatat ttgacttccg gagcctcgtt ggtgcgctcg cgcaggacga tggcctcgtt
     7261 gtaggcgttg cggaagatgc agtcgcggaa gaggatggtg tcggcaatgg tgaactgatg
     7321 gccgcgcaga agggattcct tggaatcgtg cagccagcaa tcctcgacct tgagagtgaa
     7381 atggaaggag tcggctgcgg cagcgacatc ctccgagcgg atgatgtttt tgacaaaggc
     7441 ggcgccaccc tcttcggcgc ggccatcgag gtttaatccc atgaggtgga ggctgccgcc
     7501 ggcgcgaatc tcaaagatgc gcggactacc ggccgccggg gtgatattgc gaataatcgg
     7561 tttctccgcc agccctgccg ccgcgcgaat ggtgagggtt ttggtgatgt acatcttgtc
     7621 gctgccgttg taactgtaga gcccgccgct gtcggtcagc tcgatgatgt cacccgaggc
     7681 ggcggcctcg atggcggcgt tgagggtgtt ttcgccggca gcgacctgat gaacgacggg
     7741 tccgaccggt ctgggcgccc agcgcagatc gcccatggct tcgccgtcgt cggccatggc
     7801 cagcacaggc gagccaggca gcagggtgaa atcccaggcg gcagcatcgg tgtagagggg
     7861 atcggcgtcc agcatgccgg cgccaatggc tgcggtgtca ttcagcttga ccgctgcgac
     7921 attgaaggta tcgcagtagg cgatcgtcga ggtgccgaag agctcgaccg aggtggagaa
     7981 agtgccgccc tggttggaaa agatcgaatt cttcacttcg acatcggtaa ctcccttggg
     8041 attgagaatg cgcttgttct cgcgatagga aaccgaatca aaggtgcagt ggttgatccg
     8101 gaccaccgga ttggtgtact cgacgtagag ggcttcacgg ccgattttgg caaaggtgca
     8161 gttctccagt tcgagatatt tgacttccgg ggcctcattg gtgcgttcgc gcaggacaat
     8221 ggcctcgttg taggcgttgc ggaagatgca gtcgcggaag aggatggtgt cggctgtggt
     8281 gaacatgtgc ccgcgcagca aggactcttt ggaatcgtgc agccagcaat cctcgacctt
     8341 gaggacaaag tggaaagagt cggccgcagc ggcgacatcc tcagagcgga tgatgttctt
     8401 gacaaaggcg gcgccccctt cttccgcacg accgtcgagg ttcaatccct tgaggtgtag
     8461 attgccgccc ttgcgaatct caaagatgcg cggactgccg gccgccgggg tgatattgcg
     8521 aataatcggt ttctccgcca gtccctcagc cgcgcggatg gtgaggctct tggtgatgta
     8581 catcttgtcg ctgccgttgt agctgtagag accgccgctg tcggtcagct cgatgatgtc
     8641 gcccgaggcg gccgcctcga tggcggcgtt gagggtgttc tcgccggcag ccacttgata
     8701 aaggatctgc gccggagcgc ccccggccgt caacagcagc gccagcagca ggaagaggga
     8761 gagataaaga gtttttttca tcattttctc ctggatttga tgattaggat gctatggctc
     8821 acttcatcaa aatgaattta cgggtggcct gaaagctgcc ggagcgcagc tggtaaaggt
     8881 aaatccctga ggggagatcg gcggcatcga agttcaggga gtgcgcccct gctgtcagtt
     8941 ttttctccag gagggtgcgc agcttctgcc cgagcatgtt gtagagcacc agggaggtca
     9001 tcccctcgcg ttcgagcgaa aagttaagag tcgtcgaggc attgaaggga ttgggacagt
     9061 tctgcgccag ggcgtagacg gcaggttgga gcggggcggc ggcgacgccg gtggactgct
     9121 caaatttgac agctatgctg gtcgacccat tgaccgtaac tgtcgccgga ttggccgctc
     9181 cggttatcgc tccgctccag ccggcgaact gccaggctga atcaggcacg gcagtgagag
     9241 tgacggtggt gccggtggga tagttggggg cgatcagggg cggatcgcag agcaccgttc
     9301 ccatgccctc gatggtgagg agaaagggca cgcgcgtggc cgtctgggtc gcccagcgca
     9361 gatcgcccag ggcctggccg tcgtgggcgc tgccgtaggc gggcgaatcc gccaacaagg
     9421 tgtaatcgcc ggccaaagta ttgcggtaga ggggatcgaa ggcatagatg gtggcaccat
     9481 tcaccttgcc ccccttgctg gcgctgatgc ggctcgagta ggggactgca aagatcaggt
     9541 tgagggtgtc gatatggctg atcgtcgaac ccggaagctg cacctggacg acataatcgg
     9601 cgcgttccgc ccggtagggc ttgggcaggt ggctgttggt gacgaggatg tcgcgaaaga
     9661 taacattctt gttgttcttg aaataggcca tgcgcgttcc gcagctgtcg acggtgaggt
     9721 gctcgatcag cacataggcg tccgccgacg aggtgtcggt gtcggcatag aaacggacgc
     9781 attcggcgaa gacattttta aaggtgcagt tgcgcacgat caggctgtcc agcgcacggg
     9841 tcaccttgta tttttcggtc tcggcgatgc ggatggcctc gtcgccgatg ttgcggaagg
     9901 tgcagttttc gatttttaca ttccccgcct tgatgaccgg ctcgccggag gccggggcga
     9961 gaaaataaaa ggcatttcca cccgcatcat tctcccagtt gggcgggaaa aagtcccgga
    10021 actcgcagtc gcggatggtg atgttcaacc cctcgcgggc gaagatgcgc gggatctggg
    10081 tggccggacc gtggccgaca cgcaggccgt atttcattgg acgtttggcg ttatagccgt
    10141 caaagatgat gccctggaag atgacgtcat cgtggatgcg gaagatctcg atgacgctgg
    10201 aatcgagatc gccgtgacgg aagaccggtt tctcggcgag ccccggcttg gccattatga
    10261 ccacgggctt tttgatctgc aggtgcatgg tatcggcggt ggtgtagatc ccgccgctgg
    10321 tgaccaggag gatggtgtcc accctggcgg tgaagacata gtcgagggcc agcttgaaat
    10381 cgtactgggt gtccacctcg atcttgctcg cttgggcggc gctagcgagt gcgagagcgg
    10441 ccatcaacag gatggccatc acattgccaa gtttcatctt gctgcctcct aaaattggta
    10501 agtatagccg gccaccagat tgctgaaggt ctcggtgacg taattggcat tgctgtaata
    10561 cgagtcgtag taattcattt tcctgctcgc catggcgagt tccagacggc cataacgggc
    10621 cagggtgatg gcggttccca gggaccaggt gtcggccgag ggaccgcggt cctttatggc
    10681 ggcgccgtcg gggacaaaga gcgcggtggt ctgccggaag cctgccagca gttccagaaa
    10741 gggcaggggc tgccaggaga tgccgcagcg gaggtcgctc tggtccggaa gcacgcggtt
    10801 ggtcggatca tcggcagtga gatcgatccc accctggctg taaggggtga actgatagtc
    10861 cagaaaaagg cggaaagggt cgatgggctg aaacgcggct ccgaaggcat aggagaccgg
    10921 gtggcggaaa tcggctgtgc cggccgttgc catcgcttcc ggcccgccgg ctgtggttgt
    10981 gaccgtggag gcgcgccatt tccggctgat ggtacagggc atcttgacct ggaggccgag
    11041 gctgaacctc tccagcttta caagtgcacc aagagtgtac tgtagggcgc gatagtcgga
    11101 agttccccgg gtcacgacat cgagggtgtc gtaggcgaag cgaaagcggt tatccttggc
    11161 gatgtcgaac cagccaaccc ggcggagata ctgtaaatcc tccgattgtc cgtcgagtag
    11221 ggcggcggag agacccagct ggatccggtc aagaggcgcc caggccaggc cggcggtgaa
    11281 ggtatggaga tccccttccc tctgccgtaa aaaacgcgac catgaaaaac gcacggtgtc
    11341 gtttaccaca cgcggcacgg tgccatattc gtcgtagcca atgtggggat caaggaaggt
    11401 atcgttacgg tcgaaatcgt cgatggcgct gcgggcatat ccggctgaga cgagcacttt
    11461 ctggctggag atccgcaggg gccacgcggc cgaaatctgc tggaggccgg agcgcttcag
    11521 atcacgctgc cagtcggctg ccgccgcgct gaagggttcg aggccgagcg caggttcatc
    11581 gatgcggtag gaggagtcct gggccagctc aaaatcccag atgccgttgt cggcgggatc
    11641 gggggtgtag agcccctcaa ggtaaaaggc gagggtccag aacatgcggt tgggccggta
    11701 gacctggttc tcgcgccagc ggggggtggc atgactgagg gagagggaga tctggggctt
    11761 ctcgattccg gccaggccgg cgggattgaa ccagagtgcg ccggggtcgc cgctctgggc
    11821 ggtgaaggcg gcgccgaggg cgagggccct gacaccgggg ggctgattga gcggcagccc
    11881 ctggaaagcc aggttgtcac cgcggtgctg ggccggcgca gccgcggcga aaagaaggaa
    11941 aatcaaggtt gtttttctga acatatcagg atcgctcctc tggcaagggg attccgcacc
    12001 cgcgggcgtg cggaggggtg taaaccggcg gcatgccggc ttcagttgac gacaaatcca
    12061 aaggtgaagc gatggacggc atcgagccag gcgccataat cgctgtaggc ataatcgatg
    12121 cgcatccggt tccaggctag tccgaagccg agggagaggc cttcggtgtc gtaattcagc
    12181 ttgtagcccg cgcgcaggaa aagcagccct tcaaagccgt actccagtcc aaagttgtac
    12241 tgctgatcat agtcccgcgg ctgcaccagg tcgaaggcca gcagcagggt ctgatttcgg
    12301 gaatggatga gggaatgacc gctggccgag atcagccagg cggccatgcc gatattgaag
    12361 gtctgcggca gggggtagcc cttgccgttg tagcgctggt agtaggtcga gtcagtcgaa
    12421 gcgtcgtagc ggggcaggct gacctgatca taaaatttca cctcgggacc gaaatggcgg
    12481 atggctgcag cgatctgcag ccccatgaaa ccggtgtcgt aggtcagacc gccgtcgaag
    12541 aggagggtgc cggcgctgcg cacgccgaga tcttcgcggg catatttggc cgagaggccg
    12601 aaggcaaact tgtcggtcag gtcgcgggcg aaggaggcgc cgatcagagc cgagcccggt
    12661 tctatggtct cgccggtgta accgaggtag acgccgtcgt aaaaatcgag cgcctccacc
    12721 gaggtgacgc ggatggagcc gatatcagtg gcgatggcgt gcaggccgaa ggtgccgaga
    12781 ttgggtacgc tccaggcgcc cgagacggcg acgtgggaga tgtcgaggaa atagtccata
    12841 taatcgaaag aaatatccat ctttcggatg cgggtcaggg ccgcgggatt gaaaaaggca
    12901 gcatcggaat gatcggccgc ggcggagaag gcctcgccca tggcgcagcc gcgcgcactg
    12961 gccggcacct tgagaaactg gaaggcggtg gtgccgacct tctcgaaggc caggatcggc
    13021 cctgctgtca tgaggaaaac aaaaaggagt cttgtgatct tgccgttcat ggttgaacct
    13081 cgcgaatagg acgactggtt cagacaaaaa tcgggagaga caagcaggga tcgctatgcg
    13141 ctgcctgtac ccgcaacgtc attccctcat ttgacgatga tgaacttgcc gctcaccctg
    13201 tcgcctccgg gcgtggtgat cacgtaaaga tagatcccgg aggcgatctc ctgttcgttg
    13261 cgggtgacct gaaaccaggc cgtggtatag accggctcgt tgtgctcgat ggtctggatg
    13321 agctggccgg cataagagaa gatgcggatg gtgcatcttt cgggcaggcc gtaaaagccg
    13381 atcttgtcct cggcgccggt cttgccgccg aatcccgatt cgatattgaa gggattggga
    13441 gcgacaaaaa cctcgcccag tttggcgaca gaggcgacat tcttgtcgtg ccgggtgatg
    13501 ttggtgcggc cgctgctgtg gccctggtca ttcacagaga ccaccgagta gtaccgccgc
    13561 tcgccgagct tgaagaccgg atcggcgtcg atatattcat acagtcctga gccggtggtc
    13621 tgcccggcgg ggagggtggc gagcagggcc caggggccca taccggcgtc gctgcggtag
    13681 atgcggtact cgcgcaaggc gcccgtcagg cggggatggg tgaaacggtc cggaccatcc
    13741 ttccaggtga ggcgcacctg gccgatgctg gtgttctcga ccatgatcac cggggcgggc
    13801 acggggaccg gaatgcgata gtcgccgtcg cggggattct cctccggcca gaccggtagg
    13861 gcgatcgagt cggcgccgag ataggcctgg aaggccttgt gcgccacatc ggtcacggtg
    13921 atgacctccg aattgatgta atcgggatag ccgaaatcgt cgagatagtg ttcggtgacc
    13981 acttcgccgg cgaaggtgac gcggcggttc caggaggggg tggccgacca ctgcaccaga
    14041 gcctggccgc cctcgaccac cttgcccggc gctgcgccaa aacccaccac ctcggccaga
    14101 gagaattcaa gcctgtcgcc aggtcgcagg gtatagggcc cgaagacgat gtgcttttgc
    14161 gccgcatctg aactctgacg gtagttatag gcggcgcggc cgatccagcg ggcgctcggg
    14221 ggattgggcc aggtggtgct gccctcgctg taaaccccgc tccagcgctt gtcgggattg
    14281 atcgcatcca ccaccatctt gctgctgcgg gtgttgccgg tgctcacctt gttggaccag
    14341 ggctgcttga tgtggttgtt ctcgtcgagt tcataatact tgccgagagt ggtgcgcagg
    14401 acggcgacgg cttccgattc attgcgcgca ctctcgatgg tatcaacaaa ggccagatgg
    14461 ctggtgtcat aatacagcat gcagaaacca ggagcctggg ggctgcacag tccgccgccg
    14521 ttcttgccgg tgcgggcgaa ttcgagaaag agctccttgt tgggctcacg cttggcgaaa
    14581 agggcctcgt cgctgatgtt ggtgcgcaga ttcatgttga aagagagcca atagtccgga
    14641 tcccaaaaat tgttctggtc gccgcgatac atgccgctct cgtatttcca ctcctgataa
    14701 tggcgctgat agccgtacat cgagggggcg aagccgtaga tgaagagggc cataaagtcc
    14761 ttgagcacgg cgctctgctc gatagtggcc ggattgccgt cggtgtcgcc ggtgtattcc
    14821 agctcatatt cgtagatgat catgtcgtcg taatcggggt agctccaggc ccgcgaggtg
    14881 cgggtgacgg tcactccgag cggggtcgcc caggaggcgg tgatgatctc ctccgcctcg
    14941 tcgggattgt attccgggtt aaggctgccg tccggaagaa gcgggaaatt ctcgatccgt
    15001 tccatgccga gggggtaact ccagcgcccg atgatggtct ccgaaccggc gccggtgccg
    15061 acggcgccgc aaagggcata acggcggctc tccatgcccg gctggccgtc gggattggcg
    15121 gccatataca ctccggcgcc gatgatgttg tgctggccgc tgtagcgcgt cccctgcacc
    15181 atggttgcag aacgggaagg ccattccatc agaggggtgg tggttttgtt gccttcgtcc
    15241 ccggtggtcc agggacggcc gatgtcgcct gaattgaata cggtttcatg aagcatgccg
    15301 cggctgtgga tcatgaattc cagctcctgc gccccggccg gcagcagcag cagccccaga
    15361 aatccggcag ctaggatcaa gagagagata ttcttcataa gctcctcgaa ggttggcccg
    15421 accggtcggg actaaaaatg gaagatcatc cccatccgga aatggcgggg ctcgttggtc
    15481 aggagataga gctcctgtcc ggtgacgtag ggggcgaatt cgtcatcggt caggatgttg
    15541 gcgcgatcgg tgtgccagcg cacggtgttg cggccgtggt tgaaggtgcg gctgtagctc
    15601 caggtcttct gattgagcat gttaaagcct tcaacgtagg cggtaaggct gctgcggccg
    15661 gagccgaagc gcttctccag gcgaatgcgc cagtcgcgct cgtcaggggt cctcttgctg
    15721 aacttcaggg cctggccgaa gggatcgaga gagggaggca gggtgtaggg ccggccggta
    15781 tagtagcgga aggtgttgct gaccgagaga ttggccaggg ggcggaatcc caggatctcg
    15841 aagccggact ccttgcccgt gaggtagcgt agattaatga ccatcttgtg cttgcggtcg
    15901 taatcgagaa agacatcctc gggaaagcgc tgttcggcga gatcggccag ttcctcctcc
    15961 gagggtgtgt aattgaaaaa ggtaaccggc acggcgaggt tgtcgggatt gctgttcttg
    16021 cccttagcct cctcataatt gtagcgcagg tagccgcgca gggagccggt gacgcgctcg
    16081 aaattgacat gaaagccctt gatgtcggca tagtcacggt tgatgtaggt gcggtaggat
    16141 tctccgccct cggtcttgta aaaggccgac tcgaccaggt tcttgacatc cttgtaatag
    16201 gcgctgacat cgatttgaaa gccgccggcg aggctgcgga cgataccgat atcataggcc
    16261 tgggtgttct cgggtttgag ttcaggattg ccgagggtgt agagggtcac aatgccgccg
    16321 tcgtcgttga tctcgttgta aaagagctga tagaaattag gccgctgggt gaaggtgccg
    16381 tagttgaggt gaaaaaccga gtactcggag accggaaagg agatgccgat gcgcggctgc
    16441 agccgggtga agagaccggt cttttcggtt ttttccggat cgcggagggg gtagaacttg
    16501 tcgagatagt agcgggtgtt gaggtcatag aaatcgaagc gcagacctat gttggcgatc
    16561 agcccctcaa actccatttt atcctgcaga taaagggcgc cctcgaaagg gaaggagcgg
    16621 aaggtcagat tctggaggtt ctgggggttg gtcgcgttat actgatagtc gacatcgaga
    16681 tcgtagtagt agaactgcag gccgctcttg agcaggttgt agcggttgat ctggctggtg
    16741 tagctgctgg tcagggacca ggtgcgcgtc ttctggctgc cgcggtcgtc attcgggcgg
    16801 cccacccgct gattggaagg ggcagtatag tccacccaat tgcggttgtt gccgtaatcg
    16861 tccagatact gatcatcggt caggagctcg atgcggtccc gttgccggac atcgagaacc
    16921 ttgaagttga aatcgccata gctggcgggc gaaaagaggt ggcgccattc caggccgtac
    16981 tgcagggaac tgttggtctt tttggtcacg ctgagggtgc ggtcgaagat ccagcgttcc
    17041 cagttgctgt cgagatagtt caggaaggag aaatcgtaca taacggagag tttgagcttg
    17101 tcgtcggccg agggctgcca ggtgagattg ccgagcatct gccgctccag atccgggttc
    17161 ggcgtcggta ccaggggcga gatcatgctc tgacgcatgg ccacaaacat ctgggtgttt
    17221 ttggcgacgg ggccgccggc ggtaaagtcg aagcggtagt ccatggccct ggattcgagg
    17281 ccgacgtcgc gtaccgactg cagccagaga ttgcgcccga taagggccat gcgcagggag
    17341 tcctggtggt tgaggggatt ggggggccag acgatgcgcg gcggcagata tttggcgacc
    17401 ccgtaaccgt tctcccagag gggatgaccg ggcaggaggg ggttgtcctt gagccactcc
    17461 tcgggatcat agagcagatg gaaataatcg agattttcgg gtgagtaagg gctcccgccc
    17521 caactcttgt aatagggcag ggttgaggag aattccatgc gtgaatccca gctgcttttt
    17581 ccttctttgg ctaccatgtt gaccacgccc gattgggcat tgccgtactc ggcgctgaag
    17641 ccgctggtat agacctcaac ctgctccaga gcggtgacga tgggcaggaa ggcgcgctcg
    17701 ttgctgagcg gattgacgat ggctgcgccg ccgatcaggt aggtggactc gccgacgcgt
    17761 ccaccgcgga aatgatcatc gaccacgtca gcctgcagct cgaggatgtc ggcgatgctc
    17821 tccaccccgg cgatgctgat cacctcgccg acatcgtagg tcagcttggt ggcggtgaga
    17881 tccttttcca ccttgtcggg ccggtaggcg gtgacgacaa cctgctcccc ggcgagagcg
    17941 gtcgcctcga gggcgaaatc gaggcgggtg gttttgtcaa tcttgacctg aaccttttcg
    18001 tgaagctgtt gaatgtagcc gatgtaggaa atcctgacgt tataatatcc gggggggagg
    18061 ttgaggatga aatattgccc ttgcgtgtcg ctgaccgccc cagtcggtct ttcgagggcg
    18121 gcaacccgat cgccctgcca tactgcggtg atcatcacgt tggcgccggg cagggattga
    18181 ccgttctgtt tgtccttgac cgagcccgcg atcttgcccg tctcccctgc gcgcaggggg
    18241 aaggacagta agcatatcag cagcagggca acagagccaa ggggggcttt gattcgatgg
    18301 agcatcaggg acctccgtat caaaccgtac aatacctgcg tacaggtttt cctaaagtgg
    18361 tacgctgcag tcagttcgga ctgac
//
LOCUS       DAOPWU010000026        18206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3120_length_18206_cov_4.773566, whole genome shotgun sequence.
ACCESSION   DAOPWU010000026 DAOPWU010000000
VERSION     DAOPWU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18206
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3120_length_18206_cov_4.773566"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..974
                     /locus_tag="PKM23_02830"
     CDS             228..974
                     /locus_tag="PKM23_02830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY90438.1"
                     /translation="MDIPRIHTISESAHRIHNPFTPEKLATLGAALRMHPGTCVLDLG
                     CGSGEMLCTWARDFGITGTGIDMSPLFTEQAKVRAEELGVVDQVTFIHGDAAGYVAEE
                     KVGVAACVGATWIGGGVAGTIELLARSLDPGGVILIGEPYWRQLPPTEEAARACLAGA
                     IADFLLLPELLASFGHLGYDVVEMVLADQDSWDRYEAAKWLTMRRWLEENPDDDMAKE
                     VRANLSSEPVRYATYTREYLGWGVFALMKQ"
     gene            complement(1199..1753)
                     /locus_tag="PKM23_02835"
     CDS             complement(1199..1753)
                     /locus_tag="PKM23_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNY90439.1"
                     /translation="MLLSREQAIDLLRQNIKNERMIFHCLASEAVMRALARRLGKDEE
                     QWGQAGLLHDLDVEMTAADPTVHGLETTRMLTEMGIDPEVVQAIRLHNEMAAREPRTS
                     EFHHALAAGETITGLIVATSLVYPDKKISSVKPKSVVKRMKEKAFAASVRRENILECE
                     MIGISLEEFAALALTAMAALESDR"
     gene            complement(1870..3234)
                     /locus_tag="PKM23_02840"
     CDS             complement(1870..3234)
                     /locus_tag="PKM23_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNY90440.1"
                     /translation="MNLQTRREFLKAVGIGATALALPEIHCSRDRKMRPNIIYILADD
                     LGYGDLGCYGQKLIRTPNLDRMAGEGLRFTDHYAGSTVCAPSRCSLLTGNHTGHTWIR
                     GNREFYPEGQEPLPVGTTTLATLLQEVGYATGLIGKWGLGGPGSTGVPNRQGFDYFFG
                     YLCQREAHNHYPQHLWRNHERVELGGQQYSSDLFAREALDFIRRNRARPFFLYLAFTI
                     PHFALQVPEIAPYADADWPPEMQRFAAMITRMDAHIGALMALLKELGIDRHTLVMFSS
                     DNGPHQEGGARPDFFNSSGGLRGIKRDLYEGGIRVPLIARWLGTVPAGEVTRQVSAGW
                     DILPTFCEAAGRAAPAGIDGLSLMPTLRGHPEQQLHHEYLYWEFHERGGSQAIRMGEW
                     KAVRLEVSRNPVAAVELYDLKSDPHESRDVAEAHPEVTARMRELFRSARMDSELFALY
                     QSAR"
     gene            complement(3309..4520)
                     /locus_tag="PKM23_02845"
     CDS             complement(3309..4520)
                     /locus_tag="PKM23_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HNY90441.1"
                     /translation="MDRMPLQSILIKPAGPDCNMHCAYCFYCCTQALFPEPAPHRMSL
                     EVLEATIRQFMPCAGGTASICWQGGEPTLMGLPFFEQAVELQARYGWGMTVANALQTN
                     GLLIDAAWTTFLRRCSFLVGLSLDGPAHVHDRYRHHAGGGSWARVRDSAKRLLDAGVS
                     VNAMSVVSDYAARFPDEIYATLKELGLSWMQFVPCVEADAHGHAAPFTVAPEEYGSFL
                     CRIFDLWRDDFTGDRPTTSIRFFEALLYNYVGMTPPECSLMEECGSYVVVEHSGDCYA
                     CDFFVTPEWRLGHILQDDLAEMFHSRRQQEFRRRKTLLPAECRLCRWYRLCHGGCPKD
                     RLRIADDKGKNHLCLALQRFFAHADPTLRQLAGQIRNNMEASRNKGPVSRNSPCPCGS
                     GRMYKQCCGKL"
     gene            complement(4635..4925)
                     /locus_tag="PKM23_02850"
     CDS             complement(4635..4925)
                     /locus_tag="PKM23_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90442.1"
                     /translation="MKPNRRPLQGLRHCNEPGTVCHHPTEERPIGNKLTSKVILEGTR
                     LTFKTEMAFELNEHPRFVGPRKYRYHSPLISGEWCAFTNFPWGQGLINYQPQ"
     gene            complement(4882..6711)
                     /locus_tag="PKM23_02855"
     CDS             complement(4882..6711)
                     /locus_tag="PKM23_02855"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNY90443.1"
                     /translation="MIKRNAGLFLLLVLASAAQSQGQKSTHTFALGTSEFLMDGKPFQ
                     IISGELHPARIPASHWQHRIRMARAMGCNTISVYLFWNYHEQEEGIYDFTTGNRDLAQ
                     FFRLVQAEGMWLIIRPGPYVCAEWDLGGIPPWLLRIPDIKFRCMDPRYMQAVERYIGE
                     LAKVIRPFQITDGGPLLMIQIENEYGSYGNDRNYLFRLRELWQQNGMVVPTFTGDGAT
                     TYMLEAGSLPGSAVGLDSGDSEEGFALAARMNPGVPVFSSETYPGWLTHWGEEWARRD
                     TPGLLKEVKWLMDSKKSFNFYVIHGGTNFGYTAGANSGRKGYEPDVTSYDYDAPITEQ
                     GAATPKYHALRDLITSYLPKGKKLPPVPAPVPTIELASFILQPFTSLWDNLPEPVDSV
                     QPKTFEAYGQDYGFILYKTELIGHKSGRLTVTDVHDYATVFLNGSYIGTLDRREKIHS
                     IELPESQVEKPVLEILVEGMGRINFAQELIDRKGITDRVTLNGMTLMNWQVCKLPMDN
                     KFIANLRSTATPGKKPGLFFKGSFELTRDSGAAGCDTYIDLSAYTKGLVWINGHNLGR
                     YWNIGPQQRLYCPAEWLLEGINEIIVFDLHETEPQAIAGASTL"
     gene            complement(6858..6983)
                     /locus_tag="PKM23_02860"
     CDS             complement(6858..6983)
                     /locus_tag="PKM23_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90444.1"
                     /translation="MALTLPMGWNSWNLIEGEVSEQIICEIADAIAANGLQQAGC"
     gene            complement(7064..7696)
                     /locus_tag="PKM23_02865"
     CDS             complement(7064..7696)
                     /locus_tag="PKM23_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNY90445.1"
                     /translation="MAASDRKIMVVDRSTLFAEEYFQGFAPAAAHDYQSLIRDHYFYE
                     LRDLAEKMPQYKQPIAYAIIVHKESHEVFAYQRSSKEGHYNESRLRGKWSWGIGGHID
                     KIDIENGDPILASLLRELSEEVEIKQYDDPVILGYINDDETEVGSVHFGLLYLILTSD
                     RGVRPADAEIAWGGFMPVSQLEEISQNSEASVESWSEISLTPLKQALQRK"
     gene            complement(7706..9055)
                     /locus_tag="PKM23_02870"
     CDS             complement(7706..9055)
                     /locus_tag="PKM23_02870"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNY90446.1"
                     /translation="MIKLKMPLLAPEVDAAALKAVQPAVRRVVDQLYKGRCPGSDMLG
                     WRDLPLKEDSAELRQIQKLARKIRQKADLFIVVGIGGSYLGARAAIEGLSPALPGNRS
                     GPEILFLGNHLDADYAAKLLRYIKGKKYYVNVISKSGTTTEPGIAFRLLLDHLRRTLP
                     AKKVKERVIATTSPVKGALLAMAEEFGFHKFIIPEDVGGRFSVLTPVGLLPMAVAGID
                     IGKMLKGAAGMARHCSENRTLGKNDALKYAAARHLLYLAGKPIEILGVWNPALLYLAE
                     WWKQLFGESEGKDKKGIFPASVGLTTDLHSLGQYIQDGRRDLFETFLVIDKNRRKVAI
                     PELEGDPDQLGFLAGQQLAFANQQAWRGTMLAHRAGGTPNMTLHIGKRDAFHFGELFY
                     FFEFAVAVSGLLLGVNPFDQPGVEAYKKNMFALLGEPGREEERRALEEQLLKIDDSE"
     gene            complement(9084..9551)
                     /locus_tag="PKM23_02875"
     CDS             complement(9084..9551)
                     /locus_tag="PKM23_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY90447.1"
                     /translation="MAKILIVDDEQDVLETLPEVVRKWGHETFAAKNGVEALLALQQQ
                     NFDFIVTDVKMPEMDGMELLQKIQEFDKNVMVIFLTGYPSLDSAITAMRSGAYDYLVK
                     PVNLDELKLRIERGLERKEHIKALPLLRGLNWALLVSIPLWLLLGYILTKLLR"
     gene            complement(10106..11104)
                     /locus_tag="PKM23_02880"
     CDS             complement(10106..11104)
                     /locus_tag="PKM23_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90448.1"
                     /translation="MKIKDDYAEEVAVREEREAGRPAVWALAGAAPHSGSGIAAFLLG
                     GVAARSGREVLLFRPGQWETPPKQHHPPNQPLHREEMTAVLGRLSTGELASAEGLYHH
                     LLHLDPQQDPAPLWLLDLGCSRSHAHWDLFWSCDLALLVTDRREESDLRRMAGSAWQR
                     WLERALALEDESLHAQILASPPPQPGRESQDYFTSSRLRQLGRQDPLLYIVIDKPATD
                     RGWPKEGATAPAPETPLKSAGVLSCAGATALMIRDLHAQFNLLPHAAQAWLLNRLQAR
                     LNQASPGSSAQSGEKARTIQHGFYTRLEGRQILNIDLNFFDVAAVVSPFAATDDEL"
     gene            complement(11417..12778)
                     /locus_tag="PKM23_02885"
     CDS             complement(11417..12778)
                     /locus_tag="PKM23_02885"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY90449.1"
                     /translation="MDQAIKILIVDDEENCLRSLRDVLELENISCTTTSSGLKGLELF
                     AHDEFDIVITDVRMPGVSGIDLLRQVKRLRPETFVIMLTGYGSINDAVASIKMGAYQY
                     ILKPVIMDDLFALIKDITGQINRTAGSSPLMDLKEPLPVGKTILGRSIRLQNVAKLIS
                     KIASTDLPVLILGESGTGKELAATAIHHTSERAAKPFVAINCAAFPDTLLESELFGYE
                     KGAFTDARTSKAGRIEEADGGTLFLDEIGDMKPAMQAKMLRVLEEQEFQRLGSNKSRR
                     IDVRLISATNRDLSQAIAEKGFRTDLYYRLNAVTIAMPPLREIPEDIPLLANHFARDF
                     AARFNRESISVEEEAIAAMQRYPWPGNVRELANAIRRAVALSEGPTIRIYDLPPHIIQ
                     EVAPSYEIEPAPSGVLLLEEVEKEHILKVLEMTDGNRSEAAQMLGIHRDTLLRKLKKY
                     GVE"
     gene            complement(12845..14467)
                     /locus_tag="PKM23_02890"
     CDS             complement(12845..14467)
                     /locus_tag="PKM23_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY90450.1"
                     /translation="MKLKIFYIIIQDNSEAKVPIIRMVNNPDKTPILLVEDDPDQAQV
                     LSYFLGNNGYAVDWQNAPRLAYEAARSKSYQLILLDIMLNAEEDGFDLCRQFKQEEGL
                     KSIPIIMVTARADVKDRVSGLRAGADDYVIKPFSQEELLARVQAVLARKHYFDFNEKY
                     RALLENSDDIVLFLNLEGEIEQTNHQAAVKLKGLTRPGADKFRLVTLFAEPFAANLRQ
                     LFQRVAAGFDVSGNNWRLQAPLSGMEIVDVRLVPLSQGRRISGMGCILRDATPREKVL
                     QEMEAQKRDLASHLKHTSDQLGEMQQQLILSEKMAVMGQLAAGVAHELRNPLNIISTS
                     VYYLRRILAGDHPKAKEHFDILEEEIARSQRIISSLLDFSRKSPPEQSDIDINAVLGQ
                     TLALVKKELAMNDILVRTEFAPSVKAWVNVDDLKQIFLNLILNAKEAMPYGGKLYIRT
                     LNADDHSVQIQFSDTGVGIPENIQDKIFDPFFTYGKEGKGTGLGLTIVHSAVERNGGQ
                     ISIHSELDKGATFRISLRRRAGESEKKAEKAD"
     gene            14667..15098
                     /locus_tag="PKM23_02895"
     CDS             14667..15098
                     /locus_tag="PKM23_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNY90451.1"
                     /translation="MAINSRHRASVVTQGKINPNTPFNDEELNYFRQLILERKAAALE
                     EIERLEKYLAAGNEEGSTDSAYSYHMADAGTDTMHLDYVYIQIERQKSLIGYLDRALE
                     RIKNKTYGVCRVTGSPIPRERLEAIPHTEISVEAKMQENRR"
     gene            15344..15973
                     /locus_tag="PKM23_02900"
     CDS             15344..15973
                     /locus_tag="PKM23_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNY90452.1"
                     /translation="MKIRSAVALMLALLVGAGMAQTNNTFSVYTGYTMSAFEDQEDAA
                     GTLPLGIQFGFNVAPNVEVGLEAFNAIGGYKFEADFFGVETETAFNSTIISGYGKYYV
                     SQANLSPFIKAGVGYFLGDANIKMSYMGEEEEDDMKIDPAIGFILGAGVDFNKNFFAE
                     FNYNIVSRKSGEDTDEGDFELMKSAAEDESDDSWGMNAWTIKVGYRFQL"
     gene            complement(16028..17092)
                     /locus_tag="PKM23_02905"
     CDS             complement(16028..17092)
                     /locus_tag="PKM23_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90453.1"
                     /translation="MPVFYKNATVTMDYLPALACLIWSYLFLQERAPDKAALLVGLST
                     GFRPTGLLFMLPILLHLRLTGHSWRALFRAALLCAAAALLACSPLFFAQEFGAPAPAP
                     RSSLLQHIGLIAFHGLRFLGVVQSLLLLLLLMFTLRRRPQSHAPAGFTPFHLLNIALW
                     AGLFLLLPDEPEYMLPALPSLLFLLDRFLEHRSFAATAAILLSYHVIQLELRPELPDA
                     LQLRPRLVAGYTVEDLSDRIFKLSSRRIATQYRPEQPTCLMFGLPWIPAVNPDWVYDA
                     ELGVYKQKEGEFCLSGRITDRGQIERLKQRGMRLVAWRLAVWDYLRTGSAAWGDDVEL
                     LEDLRTLFPERIRGKALNER"
     gene            17123..17251
                     /locus_tag="PKM23_02910"
     CDS             17123..17251
                     /locus_tag="PKM23_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90454.1"
                     /translation="MAKVAGAGEAAQGQKDEQSGGEGEEIAERPAAEGDQGVITSS"
     gene            complement(17436..>18206)
                     /locus_tag="PKM23_02915"
     CDS             complement(17436..>18206)
                     /locus_tag="PKM23_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HNY90455.1"
                     /translation="PVRSTTALRTECTIPFRIDIAGGWLDQPFVSKHHPGAVLTISIE
                     PTVEFNERSGMASSTRRKAIELWGNEIPHGSYEQLARILFSFENPPGTREVAGSQDAL
                     GIVFPGLNRHDYDNHYWPLKITTVDDEGLLTWLEKHLWLVTLGPRVSSYNVLSDTRID
                     REGAEALAEATDECWQAILTRDLSAFGTAVRRSFEAQVAMFPNMVDEAVRSTIVQYRD
                     QALGWKLSGAGGGGYLIMVSRQPIEGAMQIRIRRKNTL"
BASE COUNT         3570 a         5112 c         5818 g         3706 t
ORIGIN      
        1 acgcctatat cgaacaaaat aactggacgc atatcagaca gttactcggc tatgcccgct
       61 ttggtaaacc cgaactggtt ggaatgctta atgatctcta cgaaaatgaa tggcgactcc
      121 tgctaaactt ctttataccc tatgtcaaac tgctcgataa aactcgacaa ggctccaaga
      181 ccacaaaaaa atatgatcct ctcacaccca tcaggagaac gaaaacattg gatattccac
      241 ggatccatac catcagcgaa agcgctcacc gcatccataa cccgttcaca cccgaaaaac
      301 tcgccaccct cggcgcggcg ctgcgaatgc atccaggaac ctgcgtgctc gacctcggct
      361 gcggatcggg ggagatgctc tgcacctggg cccgcgactt tggaatcacc ggcaccggca
      421 tcgacatgag tccgttgttc accgaacaag cgaaagttcg cgctgaggag ctcggtgtcg
      481 ttgatcaagt cacctttatc catggtgatg ctgccggtta tgtcgctgaa gagaaggttg
      541 gcgtggccgc ctgcgtcggc gccacctgga tcggtggggg agtcgctggc actatcgagc
      601 ttctggcccg gagcctggac ccgggcgggg tcatcctcat cggcgagccc tactggcggc
      661 agctgccccc gaccgaagaa gccgccaggg cgtgtcttgc cggcgccatt gcagattttc
      721 tcctgcttcc ggaactgctc gcctctttcg gccaccttgg ctatgacgtc gtggaaatgg
      781 ttttggcgga ccaggacagc tgggatcgat acgaggcggc caagtggctc accatgcgcc
      841 gatggctgga agagaatcct gacgatgaca tggcaaaaga ggttcgcgcc aatctgtctt
      901 cggaacccgt gcgctacgct acttacacac gggaatacct gggctggggc gtgttcgcgc
      961 tgatgaagca atgatgtaca ggtgaccctg ttgactggag gtcattttta ctggctgcaa
     1021 atccggaact gacaatgcag tgcaacgacc cgctgccttg atttcttcca cagccgcctg
     1081 caaaaagagg ccggctggat attgttcggg ggcattgtga ggccgccggt ctgcctggcc
     1141 gcagtcgccg cagtcgccgc tggcggactg gtgtgccctg gatgccgcag cacttccgct
     1201 aacggtcact ctccagcgcg gccatagcgg tgagcgccag agcggcgaat tcctccagtg
     1261 atatcccgat catttcgcac tccaggatat tttcgcgtct cactgaagcg gcaaaggctt
     1321 tttccttcat gcgcttgacc accgatttcg gcttcacgct ggagatcttt ttgtccgggt
     1381 agaccagtga ggtcgccaca atgagcccgg tgatggtctc gccggcagcc agggcatggt
     1441 ggaactcact ggtccggggc tctcgggcgg ccatttcatt gtgcagccgg atggcttgca
     1501 caacctccgg gtcgatgccc atctccgtga gcatccgtgt ggtttccagt ccgtggaccg
     1561 tgggatccgc ggcggtcatc tccacatcga ggtcgtgcag caggccggcc tggccccatt
     1621 gttcttcatc ctttcccagc cgcctggcca aagcgcgcat cacggcttcg gacgccaggc
     1681 agtggaagat catgcgctcg tttttgatat tctgccgcag cagatcaatg gcttgttctc
     1741 gggacaggag cataggtttt ggtctcgcta tttcgttgtg caaaggcgct tctattatca
     1801 gggcatacct gtggtactgc tgccgatccg gagctgacaa ctccgcctgc tccttttctg
     1861 cagaatcggc tatcgcgccg attggtagag ggcaaataat tccgaatcca tccgggcgct
     1921 gcggaaaagc tcccgcatcc gggcggtcac ctcggggtgg gcctccgcaa cgtcccggct
     1981 ctcgtgcgga tcgctcttta gatcatagag ctccacggcg gcaaccgggt tgcggctgac
     2041 ctccaggcgc accgctttcc actcccccat gcggatggcc tgactgcctc ccctctcatg
     2101 gaattcccaa tagagatatt catggtggag ctgctgctcc ggatggcctc gcagggtcgg
     2161 catgagcgag agtccgtcga tgccggcggg tgccgctcta ccggccgcct cgcagaaggt
     2221 gggcagaata tcccacccag cggatacctg ccgggtgacc tctccagccg gtaccgtgcc
     2281 cagccagcgg gcgatgaggg gcacgcggat gccgccttcg tagaggtcgc gtttgatccc
     2341 gcgcagaccg ccgctgctgt taaagaagtc cggcctggcc ccgccctcct ggtgcggacc
     2401 gttgtccgag ctgaacataa ccagggtatg gcgatcgatt cccagttcct tgagcagcgc
     2461 cataagcgcg ccgatgtggg cgtccatgcg tgtgatcatg gccgcgaaac gctgcatctc
     2521 tggcggccag tccgcatctg catatggggc gatctccggg acctgcaggg caaaatgggg
     2581 gatagtgaag gcgaggtaga ggaagaaggg ccgggcgcgg ttgcggcgga tgaaatcgag
     2641 ggcctcgcgg gcaaagagat cgctgctgta ctgctggccc cccagctcga cgcgctcatg
     2701 gttgcgccag aggtgctgag gatagtgatt gtgcgcctcg cgctgacaga ggtagccgaa
     2761 aaagtagtcg aagccctggc gattgggaac cccggtgctg ccggggccac cgaggcccca
     2821 cttgccgatg aggccggtgg cgtagccgac ctcctggagg agggtcgcca gggtcgtggt
     2881 gccgaccggg aggggttcct ggccttccgg ataaaactca cggtttccgc ggatccaggt
     2941 gtgaccggtg tggttaccgg tcaagagcga gcagcgcgaa ggtgcgcaca cggtgctgcc
     3001 ggcatagtgg tcggtgaaac gcaagccctc gccggccatg cgatcgagat tcggggtgcg
     3061 gatcagcttc tggccgtagc agcccagatc gccatagcct agatcgtcgg cgaggatata
     3121 gatgatgttc ggacgcatct ttcgatctcg cgaacagtgg atctcgggca gagccagcgc
     3181 ggttgcgccg atacccacgg ccttgaggaa ttcccgcctg gtctgcagat tcatgccctc
     3241 ctccctttat gattgcacag caataaattc cacaaaccct ctcaccagcc gcccggtgct
     3301 ggggcgaatc agagcttgcc gcagcactgt ttgtacatgc ggccgctgcc gcaggggcag
     3361 ggtgaattac ggctgaccgg gcctttgtta cgcgacgctt ccatgttatt ccggatttgc
     3421 ccggccagtt gtcgcaaggt gggatcggca tgggcgaaaa accgctgcag agccaggcag
     3481 agatgatttt tccccttatc gtccgcgatg cgcaggcggt cttttggaca gccgccgtgg
     3541 catagcctgt accaccggca gagccggcat tccgcgggga gcagggtctt gcgacgccgg
     3601 aactcttgct ggcgccggga atggaacatc tccgccagat cgtcctgcag gatatggccc
     3661 agcctccatt ccggggtgac aaagaaatcg caggcgtagc agtcgccgct gtgctcgacc
     3721 accacatacg acccgcactc ctccatcagg gagcattccg gcggggtcat cccgacatag
     3781 ttatagagca aggcctcaaa gaagcggatc gaggtagtgg ggcgatcgcc tgtgaaatcg
     3841 tcacgccaga ggtcaaagat gcggcagaga aagctcccat actcctcagg cgcaaccgtg
     3901 aaaggcgcag catgtccatg ggcatcggcc tcgacgcagg ggacaaactg catccaggag
     3961 agtccgagtt ccttgagggt agcgtaaatc tcatccggaa agcgagcggc ataatcgctg
     4021 accacgctca tggcgttcac ggagactccg gcatcgagca gccgtttggc gctgtcgcga
     4081 acgcgcgccc agctcccgcc gcctgcatgg tgacggtagc ggtcatgcac atgggcgggg
     4141 ccatccaggg atagcccgac gagaaaggag cagcgccgga gaaaggttgt ccaggctgca
     4201 tcgatgagga gtccgttggt ctgcagggcg ttggcgaccg tcatccccca cccgtagcgc
     4261 gcctgcagtt caacggcctg ctcgaaaaag ggcagcccca tcagtgtcgg ctcgccgcct
     4321 tgccagcaga tcgaggccgt ccccccggcg cacggcatga actgcctgat tgttgcctcc
     4381 agcacctcca ggctcattcg atgtggtgcc ggctccggga acagcgcctg cgtgcagcaa
     4441 tagaagcagt acgcgcaatg catattgcag tccgggcccg cgggcttgat caggatactc
     4501 tgcagcggca tcctgtccat ggcgttgttc atgaaagcat cgtctgtcat ttacctgtta
     4561 acagagtgaa agtacatcat cccacgcgcc atcgccgctt gtctgccggt ttggggcaag
     4621 aatgccgccg ctccttactg gggttgatag ttaatcagtc cctggcccca gggaaagttg
     4681 gtgaacgcac accattcgcc ggaaatcagc ggcgagtgat agcgatattt gcgcggtccc
     4741 acgaaacggg gatgttcatt caactcaaag gccatctccg tcttgaaggt gagccgggtg
     4801 ccttcaagaa tgactttcga cgtcagttta ttgcctatcg gccgttcctc tgttgggtga
     4861 tgacagacgg tgcccggctc attacaatgt cgaagcccct gcaatggcct gcggttcggt
     4921 ttcatgaagg tcaaaaacga tgatctcgtt gatgccctcc agcagccatt cggcggggca
     4981 gtagagtctt tgctggggtc caatgttcca gtagcgcccg agattgtgtc cgttgatcca
     5041 gaccagtcct tttgtatagg ctgaaagatc gatatacgta tcgcagccgg ccgctccgct
     5101 atccctggtc agctcaaaac tccccttgaa aaaaaggccc ggtttcttgc cgggagtagc
     5161 ggttgatctc aggttggcga tgaatttgtt gtccatcggc agtttgcaga cctgccagtt
     5221 catcagggtc atgccattca gggtgacccg gtcggtgata cccttgcggt cgatcagctc
     5281 ctgggcaaaa ttgatgcgcc ccatgccctc gaccagaatt tccagcaccg gcttttccac
     5341 ctggctttct ggcagctcga tggagtggat cttttctctc cggtccaggg tgccgatata
     5401 gctcccgttg aggaatacag tggcataatc gtgcacatcg gtcacggtga gcctgcccga
     5461 tttgtgaccg atcagctccg ttttataaag aatgaaacca tagtcctggc cataggcctc
     5521 gaacgttttc ggctgaacag aatccaccgg ttccgggaga ttgtcccata gcgaggtgaa
     5581 gggctgcagg ataaatgagg ccaattcgat cgttggaacg ggtgccggaa cgggaggcag
     5641 ctttttacct ttcgggaggt aggaggtgat gagatcccgg agggcatggt atttcggggt
     5701 tgcagcgccc tgttcggtga tgggggcgtc gtaatcgtag ctggtcacat ccggttcata
     5761 gcctttccgg cccgagttgg cgccggcggt gtagccgaaa ttggttccgc catggatgac
     5821 ataaaagttg aaggatttct tgctgtccat cagccacttc acctctttga ggaggccggg
     5881 ggtgtcccgc ctggcccact cctcgcccca gtgcgtcagc cagccggggt aggtttcgct
     5941 gctgaagacc ggcacaccag gattcatcct tgccgccagg gcgaaaccct cttcagagtc
     6001 gccggaatcc agtcccacgg cgcttccagg caggctgccc gcctcgagca tataggtggt
     6061 cgcgccatca ccggtgaagg tgggcaccac catcccattc tgctgccaaa gctccctgag
     6121 acgaaacaga tagttcctgt cgttgccata gctgccatat tcgttttcga tctggatcat
     6181 cagcagtggt cccccatcgg tgatctggaa gggtctgatc acttttgcga gctcgccgat
     6241 gtagcgttcc acagcctgca tgtacctcgg atccatacag cggaacttga tatccggtat
     6301 cctgaggagc cagggcggaa tgcctcccag gtcccattcg gcgcagacat aggggcccgg
     6361 ccggatgatc agccacatcc cttcggcctg gacaagccgg aaaaactgcg cgagatcccg
     6421 gtttccggtc gtgaaatcgt agatcccttc ttcctgttcg tgatagttcc agaagaggta
     6481 gacggagatg gtattgcagc ccatcgccct ggccatcctg atccggtgct gccagtgtga
     6541 ggccggtatt cttgccgggt gaagttcgcc gctgatgatc tggaagggct tgccgtccat
     6601 gagaaactct gacgttccca gtgcaaaggt gtgtgtactc ttctgcccct gcgactgggc
     6661 cgcagaggcc agcaccagca agaggaacaa accagcgttt cgcttgatca tggtaagcct
     6721 ttttaaaata tttacctggt attaaagaca tccggcgaga agcgccaaaa ccctcaacag
     6781 atccaaatcg tacctgcttt tcatggccat agaccatacc agacaatctg ctctcctttt
     6841 gtcggcaaat taatccatta gcagccggcc tgctgcaggc cgttggcggc gatggcatcg
     6901 gcgatttcac agatgatctg ttcactgacc tcgccctcaa tcagattcca gctgttccag
     6961 cccatgggca gggtcagcgc caaccccgct gcttcctgac tgcagcaggg gaggtgctgg
     7021 agaaaagaag gatgagcgtg gatgtaaaga tggttttcaa ggattatttt ctttgcaggg
     7081 cctgcttgag cggggtgagg ctgatctcgg accagctttc gacggaggcc tcgctgttct
     7141 ggctgatctc ctcgagctgg ctgaccggca tgaagccgcc ccaggcgatc tcggcatcgg
     7201 ccggacgcac gccgcggtcg ctggtcagga tgaggtagag caggccgaag tggaccgaac
     7261 cgacctcggt ctcatcgtcg ttgatatagc cgaggatgac gggatcatcg tactgtttga
     7321 tctccacctc ctcgctcagc tcgcgcagaa ggctggcgag gatgggatcg ccgttctcga
     7381 tgtcgatctt gtcgatgtgg ccgccgatgc cccaggacca tttgccgcgc agacggctct
     7441 cgttgtagtg cccctccttg ctcgagcgct gataagcgaa aacctcgtgg ctctccttgt
     7501 gaacaatgat ggcgtaggcg atgggctgct tgtactgcgg cattttttcc gcgaggtcgc
     7561 gcagctcata gaagtaatga tcgcggatga gcgattgata atcgtgcgcg gcagcgggcg
     7621 caaagccctg aaaatattcc tctgcgaaga gggtggaacg gtccactacc atgatcttgc
     7681 ggtctgatgc ggccacgggg tctccttatt ccgagtcgtc gatttttaaa agctgttctt
     7741 ccagggcgcg cctctcttct tcgcggccgg gttcgccgag cagggcgaac atgttcttct
     7801 tgtaggcctc gacgccgggc tggtcaaagg ggttgacccc gaggagcagg ccgctgacgg
     7861 cgacggcgaa ttcgaagaaa taaaagagct cgccgaaatg gaaggcgtcg cgcttgccga
     7921 tgtgcagggt catattgggg gtcccgccgg cgcgatgggc cagcatggtg ccgcgccagg
     7981 cctgctggtt ggcgaaggcg agctgttggc cggccaggaa accgagctgg tcgggatcac
     8041 cctccagctc cgggatcgcc accttgcggc ggttcttgtc gatgaccaga aaggtctcga
     8101 agagatcccg ccggccgtcc tggatgtact ggccgagaga gtgaaggtcg gtggtcagtc
     8161 ccaccgaggc ggggaaaatt ccttttttgt ccttgccctc gctctcgccg aaaagctgct
     8221 tccaccattc ggccagatag aggagggcgg ggttccagac cccgagaatt tcgatcggct
     8281 tgccggccag atagaggagg tggcgggcgg cggcatattt aagggcatca tttttgccca
     8341 gggtgcggtt ctcgctgcag tggcgggcca tgccggccgc ccctttcagc atcttgccga
     8401 tgtcgatgcc ggccacggcc atgggcagga ggccgaccgg ggtgagaacg ctgaagcgtc
     8461 cgccgacatc ctccggaata atgaatttat gaaagccgaa ctcctccgcc atagcgagca
     8521 gagcgccctt gaccggactg gtggtggcga tgacccgttc tttgactttt ttggcgggca
     8581 gggtgcgcct gagatggtcg agcaggaggc ggaaagcgat gccgggctcg gtcgtcgtgc
     8641 ccgatttgct gatcacattg acatagtact ttttgccctt gatgtacctg agcagcttgg
     8701 cggcatagtc ggcgtcgaga tggttgccga ggaagaggat ctcggggccg gagcggttgc
     8761 ccggcagggc gggggatagc ccctcgatcg cagcgcgcgc ccccaggtag ctgccgccaa
     8821 tgccgacgac gatgaagaga tcggcttttt ggcggatttt ccgggcgagc ttttgtatct
     8881 gccgcagctc cgcactgtcc tccttgagag ggaggtcgcg ccagccgagc atgtcgctgc
     8941 cggggcagcg ccccttgtag agctggtcga cgacccggcg cacggcgggc tgcacagcct
     9001 tgagggcggc cgcgtcgacc tccggggcca gcagcggcat ctttagctta atcatggaag
     9061 cctctcataa agggaagaca ggatcaacgc aggagtttgg tcaggatgta gccgagcagc
     9121 agccagaggg ggatcgagac cagcagggcc cagttgagtc cgcgcagcag cggcagggcc
     9181 ttgatgtgct ccttgcgctc caggccgcgc tcgatgcgga gtttgagctc atcgagattg
     9241 accggcttga ccagatagtc gtaggcccca ctgcgcatgg ccgtgatcgc cgaatcgagg
     9301 gaggggtaac cggtcaaaaa gatgaccatg acgtttttgt cgaattcctg gatcttttgc
     9361 agcagctcca tcccgtccat ttcgggcatc ttgacatcgg tcacgatgaa atcaaagttc
     9421 tgctgctgca gggccagaag ggcttcgacg ccgttcttgg ctgcaaaggt ctcgtgtccc
     9481 cacttgcgca ctacctcggg gagggtttcg agaacatcct gttcatcatc gactatcaag
     9541 atcttcgcca tacacgacct cgcacacaaa gggttcctga tcagggccag gggcgaaatc
     9601 gccgcactcc cccgcagccg ccgcgttcaa gcacgaaacg ctggtggcgt tttccagtta
     9661 aatttacata tatcatcgca ataatcaagg gaaaaaaagc agcgggtatc cccgaaatct
     9721 ggctcccacc tggcaccttg tgctgcatac ccccgggtgg gagccagagt gtggggcata
     9781 tcaggaataa aagtagggga gaggggggaa aataaaaagg gacgatagtt ctcaggttgc
     9841 ggatgtcgta tgcgttcgcc tgtcgaagga tgcctttcaa gcgatgccac caggctgctg
     9901 tactcagatg ccgagctgtg ggtgcctgcg atggtgcgca acaaccgctg cgaagaacga
     9961 ctaccgtccc ttttcagtgt cgggtggaac aacggtttcc tcgcccgccg taacgggcct
    10021 ttgcctggcc aaccttccgg tcgcggccct gggccggcca gacaaaggtc ccgtcttcgg
    10081 gtacgtgtcg ggtgtgagga gaagcttata gttcatcgtc ggtggcggca aagggcgaaa
    10141 caaccgccgc gacgtcgaaa aaattcaggt caatattgag gatctgccgg ccctccagcc
    10201 gggtatagaa gccgtgctgg atggtgcgcg ctttttcccc gctttgggcg ctgcttcccg
    10261 gggaggcctg gttgagccgt gcctgcagcc ggttgagcag ccacgcctgc gcggcatgtg
    10321 ggaggaggtt gaactgggcg tggaggtcgc gaatcatcag ggcggtcgct ccagcgcagg
    10381 agagcacgcc cgcgcttttc aggggcgtct ccggtgccgg tgcagtcgcc ccctcctttg
    10441 gccatcctct gtccgtggca ggcttgtcga tgacgatata gagcagggga tcctgccgtc
    10501 cgagctgccg cagcctgctg ctggtgaaat agtcctggga ctcgcggccg ggctgagggg
    10561 gaggcgaggc caggatctgg gcgtgcaggc tttcatcctc cagggccagg gcccgttcca
    10621 gccagcgctg ccaggccgag ccggccatcc tgcgcaaatc gctctcctcg cggcgatcgg
    10681 tgaccagcag ggccagatcg cagctccaga agaggtccca gtgggcgtga gaacggctgc
    10741 agcccaggtc gagcagccac aggggcgcag ggtcctgctg cgggtcaagg tggagaagat
    10801 ggtgatagag cccctcggcc gaggcgagtt cgccggtgct gagacggccg agcacggccg
    10861 tcatctcctc cctatgcaag ggctggttgg gtggatggtg ctgttttggt ggggtttccc
    10921 attgccccgg ccgaaaaagc aggacttccc ggcccgatcg ggcggccacc cctccgagga
    10981 gaaaggctgc gatgccggag ccgctgtgcg gcgccgcgcc tgcaagcgcc caaacggccg
    11041 gccttcccgc ctctctctct tcccgcaccg ccacttcctc cgcataatcg tctttaattt
    11101 tcatccgccc gcggcagtat tgatggagca aatcgtcagg ggggcccggg cggcggctgc
    11161 agcgggtatc cagacccgct ctttaccagt gctggacata gcaaactgca taccataatt
    11221 atctgtggcc tgataagcta ttgatattta atggataatg gtataatgaa ccaggcgggt
    11281 ggaaagagga aaaggggcca acctgccgta atttacgaca aacgggcccc tttcaggggt
    11341 gtgtaaaaga taactctaat aaaatcaatt taagaaatca acgaaaaata cgacagtaac
    11401 ttgccctgaa agcggactat tccacgccgt atttcttgag cttgcgcagc agggtatcgc
    11461 ggtgaatgcc gagcatttgg gcggcctcgc tgcggttgcc gtcggtcatc tccagcacct
    11521 tgagaatgtg ttccttctcc acctcttcga ggaggagaac cccgctcggg gccggctcga
    11581 tctcgtagga gggggcgacc tcctggatga tgtggggggg cagatcgtag atgcggatcg
    11641 tggggccttc cgagagggcg acggcgcggc ggatggcgtt ggccagctcg cggacgttgc
    11701 cgggccaggg gtagcgctgc atggcggcga tggcctcttc ctcgacgctg atgctctcgc
    11761 ggttgaagcg cgcagcgaaa tcgcgggcga aatgattggc gagcaggggg atgtcttccg
    11821 ggatctcgcg cagggggggc atggcgatgg tcaccgcatt gaggcggtag tagagatcgg
    11881 tgcggaagcc cttttcggcg atggcctggc tcaggtcgcg gttggtggcc gagatcaggc
    11941 ggacgtcgat cctgcgtgat ttgttggagc cgaggcgctg gaactcctgc tcctcgagca
    12001 cgcgcagcat tttggcctgc atggccggct tcatgtcgcc gatctcgtcg aggaagaggg
    12061 tgccgccgtc ggcctcttca atgcggccgg ccttgctggt gcgggcatcg gtgaaggctc
    12121 ctttttcata gccgaagagt tcgctctcaa gcagggtgtc gggaaaggcg gcgcagttga
    12181 tggcgacgaa gggtttggcg gcgcgttcgc tggtgtggtg gatcgccgtc gccgccagtt
    12241 ccttgccggt gccgctttcg ccgaggatga gcacgggcag gtcggtggag gcgatcttgc
    12301 tgatcagctt ggcgacattt tgcagccgga tggagcggcc gaggatggtc ttgccgaccg
    12361 gcagcggctc cttcaggtcc ataagcgggg agctgccggc ggtgcggttg atctggccgg
    12421 tgatgtcctt gatgagggcg aagagatcgt ccatgatcac cggcttgagg atatactgat
    12481 aggcgcccat cttgatcgag gcgaccgcat cgttgatcga gccgtagccg gtgagcatga
    12541 tgacaaaggt ctcgggccgc agccgcttga cctggcgcag aaggtcgatg ccgctgactc
    12601 cgggcatgcg cacatcggtg atgacaatat cgaactcgtc gtgggcgaag agctccagtc
    12661 ccttgaggcc gctgctggtg gtggtgcaac tgatgttttc cagctcgagc acatcccgca
    12721 gggagcgaag gcagttttcc tcgtcatcga cgatgagaat tttgatggcc tggtccatga
    12781 ttatccgcct cagtgagagt cttttccata gttatgtaac accgcgaaca ggcgaaattt
    12841 tccctcaatc cgctttttca gctttttttt cggattcccc ggcccggcgg cgcagggaga
    12901 tgcggaaagt cgcccccttg tccagttccg agtggatgct gatctggccg ccattgcgct
    12961 cgacggcgct gtggacgatg gtgaggccga ggccggtgcc tttgccctcc ttgccgtagg
    13021 tgaagaaggg gtcgaagatc ttgtcctgga tattctccgg gatgcccacc ccggtgtcgc
    13081 tgaactggat ctgcacgcta tggtcgtcgg cgttgagggt gcggatgtag agcttgcccc
    13141 cgtagggcat ggcctctttg gcgttgagga tgaggttgag gaagatctgc ttgagatcgt
    13201 ccacattaac ccaggccttg accgaggggg cgaattcggt gcgcaccagg atgtcgttca
    13261 tggccagctc ctttttaacc agggcgaggg tctggccgag cacggcattg atgtcgatat
    13321 cgctctgttc aggaggggat ttgcgcgaaa agtcgagcag gctgctgatg atgcgctggg
    13381 agcgggcgat ctcctcctca aggatgtcga aatgctcctt cgccttggga tggtcgccgg
    13441 cgaggatgcg gcgcaggtaa tagaccgagg tgctgatgat attgaggggg ttgcgcagct
    13501 cgtgggcgac gccggcggcg agctggccca tcacggccat cttttcggag agaatgagct
    13561 gctgctgcat ctcgccgagc tggtcgctgg tgtgtttgag gtggctggcg agatcgcgct
    13621 tttgggcctc catctcctgc aggacctttt cgcggggggt ggcgtcacgc aggatgcagc
    13681 ccatgcccga gatgcgtcgc ccctgggaga gggggaccag gcggacatcg acgatctcca
    13741 tgccgctgag gggtgcctgc aggcgccagt tgttgccgct gacgtcaaag ccggcggcta
    13801 cacgctggaa gagctggcgc aggttggcgg cgaagggctc agcgaagagg gtgacaaggc
    13861 ggaacttgtc cgcgccgggg cgggtgagac ccttgagctt gaccgccgcc tggtggttgg
    13921 tctgctcgat ctcgccctcg aggttgagaa acagcacgat atcatcggaa ttctcgagca
    13981 gggcgcggta tttttcgttg aaatcgaaat agtgcttgcg cgccagcaca gcctggacgc
    14041 gggcgagcag ttcctcctgg ctgaagggct tgatgacata gtcgtcggcc ccggcccgca
    14101 gtccgctgac gcgatccttg acatcggcgc gggcggtgac catgatgatg ggaatgctct
    14161 tcaacccctc ctcctgcttg aactggcggc agaggtcgaa gccatcctct tccgcgttga
    14221 gcatgatgtc gaggagaatg agctggtagg atttgctgcg ggcggcttcg taggccagac
    14281 ggggggcgtt ctgccagtcg acagcatagc cgttgttgcc cagaaaatag ctgagcacct
    14341 gggcttgatc gggatcgtcc tcgaccagca ggatcggggt tttgtcagga ttgttgacca
    14401 tgcggatgat cggcaccttt gcttcgctgt tatcctgaat gataatataa aatatcttca
    14461 atttcaagca ctatttgcac ccgcaccgcc tcgcggtcgt gctcggcaaa ccgctttcgg
    14521 gactaaaaaa agagttgctt attgcccctt aagttggtat acttaatcca cacaataggc
    14581 attcatccgc ttttgatggc tatttggggt ggcggtcata ctaccgccga gacatctgct
    14641 gacggcaacc atgtgaggag gaggctatgg ctatcaatag tcgacacagg gcatccgtag
    14701 tgacgcaagg taaaatcaat ccaaatactc ccttcaacga cgaggagctc aactatttcc
    14761 gtcagctgat cctggagaga aaggcggccg cgctcgaaga gatcgagcgg cttgaaaagt
    14821 atcttgctgc cggaaacgaa gagggctcca ccgactccgc ctacagctac cacatggcgg
    14881 acgccggcac tgacaccatg catctggatt acgtctatat ccagatcgaa cggcaaaaat
    14941 cgctcatcgg ctatctcgac cgggcgctcg agcgcatcaa gaacaagacc tacggcgtct
    15001 gccgggtcac aggttcgcca atcccccgcg aaagactgga agccatcccg cacaccgaaa
    15061 tatccgtcga ggctaagatg caggagaacc gacgctgagg aaaccagctt cggcctcttc
    15121 acctcacctg gaaatgtact atacccggag gcctgggcag ggcatggtac acctgaacaa
    15181 tgcccctgtt tgggccacag atcccgacag ttgggtaaaa tcggcaaaaa accttacggc
    15241 cgccggaata agtccggcgg ccgtgttgtt ttctcaacca taaagggggt agtgtacggc
    15301 agcgttgcca ggcaaataga accacaaaaa caaggagata gagatgaaaa tcagaagcgc
    15361 agtggctctc atgctggctc ttttggtcgg tgcgggcatg gcccagacca acaatacctt
    15421 ttcggtttat accggctaca ccatgagcgc cttcgaagac caggaagatg cagccggaac
    15481 cctccccctt ggcatccagt tcggttttaa tgtggccccg aatgtggaag tcggcctcga
    15541 ggccttcaat gccatcggcg ggtacaagtt cgaagcggat tttttcggcg tggagaccga
    15601 aaccgccttc aattcgacca tcatcagcgg ctatggcaag tactacgtca gccaggccaa
    15661 tctctccccc ttcatcaaag cgggcgtcgg ctactttctc ggcgatgcca acatcaagat
    15721 gagctacatg ggcgaggagg aagaggatga tatgaagatt gatcccgcca tcggctttat
    15781 ccttggcgcc ggcgtcgatt tcaacaagaa ctttttcgcc gagttcaact acaacatcgt
    15841 cagccgcaaa agcggagagg ataccgatga aggcgatttc gagctcatga agagcgccgc
    15901 cgaggatgag agcgacgact cctggggcat gaacgcctgg accataaagg tgggctaccg
    15961 cttccagctc tagttatcaa ttttcagcag ctgaagggat ggcgatacaa tcgccatccc
    16021 ttttttatta tctctcgttg agcgccttgc cgcggatccg ctcggggaaa agtgtgcgga
    16081 gatcttccag cagctccaca tcatcccccc aggccgcgct gccggtgcgc agatagtccc
    16141 acaccgccag ccgccaggcg accagccgca tcccccgctg tttgagcctt tcgatctgtc
    16201 cgcgatcggt aatcctgccg gagaggcaga attccccctc tttctgtttg tagactccca
    16261 gctccgcatc atagacccag tccggattga ccgccgggat ccagggcagg ccgaacatga
    16321 ggcaggtcgg ctgctccggc ctgtattgtg tagcgatccg gcgggaggag agcttgaaga
    16381 tgcggtcgct gagatcctct acggtatagc cggcgacgag gcggggtcgc agctgcaggg
    16441 cgtcaggcag ctcgggccgc agctcgagct ggatgacgtg gtaggagagc aggatggcgg
    16501 ccgtggcggc gaagcttctg tgctcgagga aacgatcgag caaaaagagg agggagggca
    16561 gggcgggcag catgtactcg ggttcatcgg ggaggagtag aaaaagtccg gcccagaggg
    16621 cgatgttgag caggtgaaaa ggggtaaagc cggctggtgc atgcgactgc ggccgccggc
    16681 gcagggtgaa catcagcagc aggaggagca gcaggctctg gaccacgccg agaaagcgca
    16741 ggccgtgaaa ggcgatcagc ccgatatgct ggaggaggct gctgcgcggt gccggggccg
    16801 gcgcgccgaa ctcctgcgcg aaaaagaggg gcgaacaggc aagcagggcg gctgcggcgc
    16861 agaggagcgc ggcgcggaaa agggcgcgcc agctgtggcc ggtgaggcgg aggtggagga
    16921 ggatgggaag cataaagagc aaacccgtcg gccgaaagcc ggtgctgagg ccgacgagca
    16981 gggctgcctt gtccggggct cgctcctgga ggaagagata gctccagatc aggcaggcca
    17041 gggcgggcag ataatccatg gtgacagtgg cgtttttata gaaaaccggc atgaaaagca
    17101 gggtggcgat gacgaggcgg ggatggcgaa agtggccggc gcgggcgagg cggcgcaggg
    17161 ccagaaagat gagcagtccg gcggcgaggg agaggagatt gctgagcgtc cagccgccga
    17221 gggcgaccag ggggtaatca ccagctcgta aaggggaaat ccgagcgagc gggagggggt
    17281 atagatcccg ctcgtccaga gtttttccgc cgtcatggcg acgcgccagg cgtcatagtc
    17341 gctgccgtag ccgagcaaga gcaggggaag gcaaagggcg gcccagacga gcagaagcgg
    17401 tcggaaggga cgggcggatt cgtgcgcgtg cggcatcaga gggtgttttt ccttctgatc
    17461 ctgatctgca tggcgccttc gatgggctgg cgggagacca tgatcagata gccccccccg
    17521 ccggcgccgg agagtttcca gccgagggcc tggtcgcggt actgcacgat ggtggagcgc
    17581 accgcctcat cgaccatgtt ggggaacatg gcgacctggg cctcgaagga gcggcgtacg
    17641 gcagttccga aggcggagag atcccgggtg aggatggcct gccagcattc atccgtggcc
    17701 tcggccagcg cctcggcccc ctcccggtcg atgcgggtgt cgctgaggac attataggag
    17761 ctgacgcgcg gtcccagggt caccagccag aggtgttttt ccagccaggt gaggaggccc
    17821 tcgtcatcga ctgtagtgat ttttaaaggc cagtaatggt tgtcatagtc gtggcggttg
    17881 aggccgggga agacgatgcc gagggcatcc tgggagccgg ccacctcgcg ggtgccgggg
    17941 ggattttcaa agctgaacaa aatgcgggcg agctgttcgt agctgccgtg ggggatctcg
    18001 ttgccccaga gctcgatggc cttgcggcgg gtgctcgagg ccatgccgct gcgttcgttg
    18061 aactcgacgg tgggttcgat gctgatggtc aggacggcgc cggggtggtg tttggagaca
    18121 aagggctggt cgagccagcc gccggcgatg tcgatgcgga aggggatggt gcattcagtg
    18181 cgcagggcgg tggtcgagcg caccgg
//
LOCUS       DAOPWU010000027        18196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3122_length_18196_cov_5.265090, whole genome shotgun sequence.
ACCESSION   DAOPWU010000027 DAOPWU010000000
VERSION     DAOPWU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3122_length_18196_cov_5.265090"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2686)
                     /locus_tag="PKM23_02920"
     CDS             complement(<1..2686)
                     /locus_tag="PKM23_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY90456.1"
                     /translation="MRIAFSFSKAATLVVLLSLSLAFMAWGQDSVPAYFEGTGTLPAS
                     SWSAGDSLIYNGNLHSTKKLTGTKQIAMYIGKSDANKVDLRWAPVGEGCETAYTGYGG
                     VVFMQTGNYQGNGYFANYYSGQIRLFKITAGAVATSGTSVPVASAPTMNAGSLLSVQV
                     NTATGKFEYFLDGVSLGTITNTDYNLAAACYGGALLYSGELVNNDIEEMTFSTYTPST
                     STDTTPPAAATISAGSPTAGSVTVTWNATADDGATGSAATTYQLRYNTVAITDANFSS
                     STRFTTTTPKAPGSPESYTVTGLNASTRYYFALKIYDEANNPSPLSNVASATTTAGSG
                     GGGGGTPSAGWNSPIVDDFNRASLGSDWNAPKFVIENNELAIASSNGYGLAAFARTGA
                     NKSAGADSIKLSMTMGASAIYYQTQGYTPAGFALILDTPSASANGYWLRRSATKLSLY
                     AISGGSTFNSLIGEVNVTRTAPAAGQKVTAIIKATGTALTIHHYIDDVFDATLNTTLP
                     AAPPDSWYVGAVQYEDGSSNFNLDNFSVYLPNLGSASATTIAYASGNNQSAPINQYVA
                     DSLKVKVTDDAGNPVSNVVVDFAVTQGKGTIETGTFDGKIWKEVESGILGVPIAYDTT
                     AAAASGGHAIKTTYIGGYRFRTAVTIPIYNPEERTYYFHLRARTTSGQKNTVIIKYSD
                     RDSLTMDFTDLSGSWVWQRHNTSFRLPKGLQNIRLVIYDPGWDWDKIALISTGLSGPT
                     GSTMGDTGPNLSNISNAAGFAAARLKFDTDADTNVVVSAIAYRADGTTLLNGAPVNFT
                     LDPTSGPAVSMIKDPDLTDPILGTRDETAGATLTAVIQDAYGNGVPGTTVNWSLISGV
                     GAALARSSSTSDENGKASVTMTLGLVDTLFTVQAAAVNG"
     gene            complement(3001..5082)
                     /locus_tag="PKM23_02925"
     CDS             complement(3001..5082)
                     /locus_tag="PKM23_02925"
                     /inference="COORDINATES: protein motif:HMM:NF026235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNY90457.1"
                     /translation="MSLVLHRPAGRFIALLMLLILSLVPPLSAQQYQPPFPRLVFQRP
                     GGISGGAVQYFFSRYDLAIHGGAGLNAYALNDSIHALNPNTVILGTSRQGVWPGNPIW
                     PPACFIYSSYYGSLIEAAEPGDTDIRVDSTTGFKTNTIGDDIYALIGGDDWISFTGYT
                     DSTIHGIPVSGDYAINRSHAAGDSIKRPARFQGFGYLHNVTPLAPLVNSQPVWAWYID
                     QRFNPAKQDFSRFNGLFYDSFRFFFWGDDFQSTLDLDHNHLNDLTEPDKGLAWFNEQW
                     SAGIREMLPYERAKFTALHPGKPVVVGINMGSAQEGDPYPLRYCDGMEWEGFMRFAYT
                     APDLIRVNQLWEAAHDTVFTIIEDYVEKETHSRDYKKIRYGLTAALMSGAYYGMTCGN
                     EYSMSLWYDEFDVDLGYPVGPAREIPGQGGVYVRFFDKGAAICNASGQIVNISDAMIS
                     GLPGYQGSYYRFLGGQKPSFNNGQPFGSVELQGESLIPAKNNIGDGIILLSRPDTVVA
                     DIIVGTCYFNDTTPGSARARFDPVFRPVYDRANETVDFASRNRCFSQWVSSDSTGIGY
                     YYSAPALGKGWATFRPTIGVAGWYEISEWHPMVGETPSAYQEAADVPFEAVAGGVKKL
                     TGVIDQTTSYGRWNRIAILYLPEGTSSYVRISNQGSGYVAADAMRFRYLKRVTPDVIP
                     PHRPEGLQLVR"
     gene            complement(5189..6616)
                     /locus_tag="PKM23_02930"
     CDS             complement(5189..6616)
                     /locus_tag="PKM23_02930"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNY90458.1"
                     /translation="MGTTRKKLLAKGTRILDLTVMLCSLSGAVLLHAQLSMVRGVSQF
                     TPALLRPVDGLALILLLIAWNRLFAHFGLYTVRRLDERVREWLDIAKAVSLGTLVAAA
                     LTLLVAEELDNKFILLFYALALFASISSRALVREGLVFLRSRGRNLRHIVFVGSGPEA
                     VDLSQKILHRADLGYRLLGFVDDHPQNTRLWQGKWLCTLDAFPDYLCRHAVDEVFIAL
                     PISTCYEQVRRITCFCDELSIPCRVPSDWMELKTADTPAYELNGVPMLTLRNAGRGRF
                     SHLVIKRLLDVTLAGAGLLLLAPLFLVVSALIALSSSGPVFFKQQRVGFNRRRFRIYK
                     FRTMVDGAEALQAQLEHLNEADGAAFKIAADPRITPVGRWLRRTSIDEIPQLINVLKG
                     EMSLVGPRPLPVRDVNNIYEQWPIRRFSMRPGLTCLWQISGRHRLRFNEWMRLDLKYI
                     DEWSIWLDLKIMLRTIPEVVRATGE"
     gene            complement(6841..8112)
                     /locus_tag="PKM23_02935"
     CDS             complement(6841..8112)
                     /locus_tag="PKM23_02935"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY90459.1"
                     /translation="MNTIAASLAAANGRLVRSTTEAEFRYCLQQIAAFDAAIVDISAD
                     KRHFEMVQEFVNHFPTKPLIAIVAPADLNAAMQAIRLGATDYLLQPILSEEVPLALER
                     AMQLDLSPHFRIAQRLGWRRERDLGPVVGSGEEMLRTLQLVGELARKESHILFCGEEG
                     VGKTYFARLLHFLSPRRFQPLLRVACAHKTSGDLLLELFGTRGGLLQPSYDATLLLEQ
                     SHALPVSVQNRLAHFIEEQHHAVPGSGIRFIGLSTEAPDEAAENLFNRIGASLVHLPN
                     LMQRQADLPRLCQVLLRQISEDLALPERELQPEALTMLRGNALAGNVRELNTILQRAA
                     IMSRAMQIESNALQVAGLEPVPENQPLQLGVRSMQLDDVEQVLIRKALLKYNGNVSRT
                     ATALGISRGTLYNKMKKYGLAYSETDSVVPR"
     gene            complement(8393..9691)
                     /locus_tag="PKM23_02940"
     CDS             complement(8393..9691)
                     /locus_tag="PKM23_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY90460.1"
                     /translation="MQRSSRPRLAATISWILYDLANTAYSMNVVSLYFGTWIIINLGQ
                     SDFIVSFVNSFSMILVALTMPVLGDWSDHRGGKMALLGLFTGACILGTAVLGLLGNHI
                     TDLHILLPLVMAVFVIANYSYQGGLVFYNALLPAVSTPRTIGRVSGYGVAIGYMGSIL
                     GLAVAGLFVDGHFYGLRIPGLTGGGTAAAFIPTALLFLFFAIPIFIFVREPYAALPGR
                     PKWSLRESYRKTFRSLVDSRRYPGLPRFLLAKLLYEDSIETVIIYMGIYTQAVMGFTL
                     AEANQFFVIVIPSAVIGSALCGILTDHFGPKKTLTWVILLWVVTLTGVIATGSRTLFW
                     IAGCIIGMLMGSTWTSARPLLISLVPREKLGEFFGLYALSGKVAAVLGPILWSTITWS
                     LASFGPVIKYKAAIAALACNMAVGAWLLRRVPDHHQKSPR"
     gene            complement(9727..10146)
                     /locus_tag="PKM23_02945"
     CDS             complement(9727..10146)
                     /locus_tag="PKM23_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90461.1"
                     /translation="MAHFGLNRADNEEALPFQEAARREMQGMVQIGGFAAIYLFTHEG
                     LLLASQVANSSMLREDHAVEFAVLMAKFKKVIKKMSGLSPLKELVLEDGDGRRLVFRY
                     LTLFGQTALLLIVVPAQRSYRGLANRLCRLIEAQAAQ"
     gene            complement(10161..10733)
                     /locus_tag="PKM23_02950"
     CDS             complement(10161..10733)
                     /locus_tag="PKM23_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNY90462.1"
                     /translation="MHIDWFRQEIVFKIVYYGPGLGGKTTNLEYLHAHLDPALRSRLV
                     VLKSPEERTLYFDFFELALGAIKGKKPRFHLYTIPGQLYYASSRKIVLRGVDAIVFVA
                     DSQSARRQDNLDSLMDLELKLIEQGKTLAQFPWVLQYNKRDLTEIDSVDEMEQSLNFL
                     RVPHYEAVAVQGLAVFETLRGVIQLLLNKM"
     gene            complement(10735..11247)
                     /locus_tag="PKM23_02955"
     CDS             complement(10735..11247)
                     /locus_tag="PKM23_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNY90463.1"
                     /translation="MEMQQRKNGRRVVLSQTAYHQIALILEEFLSRSQARLALFADMS
                     GYPVVYRGDADTLDLAALTALAAGDFAATAEMSRLIGQESRFKFLYHEGASRSLYLCA
                     VGTDYFLMVVFDKSVALGIIRVLSHYTVEKIGRYIQELRQGGEQTHQFLDFEFRDMLA
                     RQLDRSLRPR"
     gene            complement(11263..11994)
                     /locus_tag="PKM23_02960"
     CDS             complement(11263..11994)
                     /locus_tag="PKM23_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNY90464.1"
                     /translation="MRVGIFSDVHGNLEALEQVLRTLKAEHCDEIVFLGDAVGYGPQP
                     DECVAAIAEVASLAILGNHDDAVLGRTNPFFFNDLALRAVLWTGRVITQETRAGLGRY
                     ELVGRRDGIFFVHATPVNPPAWEYLHDITDTERNFAAFTERICFIGHSHTPGGFASGA
                     EGGFYPLPGHGRITLNPRNRYIINVGSVGQPRDGDPRACFALYDQQTGIVEFHRVEYD
                     VQKTQQLMRTRRLPEMLALRLQFGH"
     gene            12131..12652
                     /locus_tag="PKM23_02965"
     CDS             12131..12652
                     /locus_tag="PKM23_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNY90465.1"
                     /translation="MSSPISQLAESIRMAIDLEKSGRAFYLLAAERTANPTGRTIFSR
                     LAKEETQHLLTFERMLDDEEGLSGWRSWLKEYPNHPPLPVFGEKAEKSIKEGSMDELQ
                     ALRVAMKQEREAMEYYGRIAESSDNEAVAGIFSFVREQEVYHHDLLQAEYDSITQSGF
                     WFDNAEFRMDGKF"
     gene            12704..13591
                     /locus_tag="PKM23_02970"
     CDS             12704..13591
                     /locus_tag="PKM23_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HNY90466.1"
                     /translation="MADKTPLILDPFQHDAAVRHFLGHFGLAPRTPDRPFLERILASF
                     ARLPYENISKIIKLNRHFLDIERIRLPEEVMEGYVRHNLGGTCFSLSYFLQSILLQLG
                     FSSWIFMADMGARPNVHCALLVQLEGSRLLADPGYLLSRAMEIHPDRSRLYETPHTGV
                     EIKFDRTRERYGLYTFDRQVKKLRYTFSDRPIPPAEFLQHWLASFYQGMMHNICLTQL
                     RPEGLIYMHGDYLQIATPEGRHKRRLKQEYPRVVSELFAIAPEWIERAQLALVENMDM
                     EKRHGIYRPTSGAESREEG"
     gene            13591..14136
                     /locus_tag="PKM23_02975"
     CDS             13591..14136
                     /locus_tag="PKM23_02975"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNY90467.1"
                     /translation="MEAAAPTPVKLICGVLYSDDALCEEALGLLAERYGRIDYRSALF
                     PFTITDYYSAEMGAPIQRFFCTFEPLIDPGKLAGIKIACNEMEAKLTVDGGRRVNLDP
                     GYMDYDKVVLASAKYKPHKIYLDLGIYADATLHYEKGHYHPAPCAFPDFKSGEYNETF
                     LAIRARYKGQLRKLLAGRIRE"
     gene            complement(14267..15559)
                     /locus_tag="PKM23_02980"
     CDS             complement(14267..15559)
                     /locus_tag="PKM23_02980"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HNY90468.1"
                     /translation="MASKLTMPKLSDTMTLGVLLKWHKREGDAVEAGEVVAEAESDKA
                     TMELEAFDAGTLLKIVVPDGGKVPVGGLLAVIGEKGEDISALLASGSEAAAVPSAPVE
                     AEAPAGPAAEEQIAAAPVPDVTPADVIITTDTVRIKASPLARKMAAAKGVDLRRVQGS
                     GTGGRIISRDLAAMPASAPAAAIQSAKAPAALSLTEFTPGEAARPDSEQPLNTMRTAI
                     ATRMTQSKTTVPHFYLTMEIDMERMIALRTELNAAQEEIKISYNDLIVKAAAAALVKH
                     PRVNGSFAGDKFLFHSQVDIGLAVALEDGLITPVVRNAAAKSVGRIAAEARVLIDKAK
                     ARKLTPDEYTGSTFTISNLGMFDIEEFSAIINPPEAAILAVGAIIAKPVVREGAIAIG
                     QRMKVTLSCDHRIVDGATGALFLKEFKAILENPALLLL"
     gene            complement(15714..16718)
                     /locus_tag="PKM23_02985"
     CDS             complement(15714..16718)
                     /locus_tag="PKM23_02985"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNY90469.1"
                     /translation="MSTLPGEQSMPELTFREALNQAMAEEMERDPNVFLMGEEVGYYQ
                     GAYKVSQGLLEKFGPQRVIDTPIAELGFAGLGVGAAQVGLRPIIEFMTWNFSLLALDQ
                     IVNNAAKLRYMSGGQIKMPIVFRGANGSAHQLGAQHSQSLEAWLCHIPGLIVMAPSVP
                     ADGKGMLKAAIRDDNPVAFFESEVAYGYKGEVPEGEHIVPIGVGDIKRPGKDVTLIAW
                     SKMIHVALEAANILAGEGIEAEVVDPRTLRPLDEQLLISSVEKTNRCVIVEEGWPYAG
                     VGAEIAYRITLNAFDHLDAPVVRITSEDVPMPYAANLEHAVVPSAQKVAAAAKKVLYV
                     "
     gene            complement(16725..17684)
                     /gene="pdhA"
                     /locus_tag="PKM23_02990"
     CDS             complement(16725..17684)
                     /gene="pdhA"
                     /locus_tag="PKM23_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNY90470.1"
                     /translation="MEKKKLLEMYRMMLLIRRFEERTAQMYGMQKIGGFCHLYIGQEA
                     VGVGAVSALRPGDQFISAYRDHGHILAMGGEPNAVMAELFGKATGVSRGKGGSMHLFD
                     VEKGYYGGHGIVAGQVPVATGMAFAAKYRKEDKVVLCSMGEGTVHQGAFHESLNLAKL
                     WRLPVIYMIENNRYGMGTPLERASAVWDLSEKACSYDMARATVDGMDVLAVHDVVAEA
                     IVRATRDGEPTLIEARTYRFRGHSMSDPVHSHYRTKEEVEEQKRQDPILIFQQKLIDD
                     KILTPEKVKEYEDEIKKIVLDAVDFAEKSPLPALEELYTEIYS"
     gene            complement(17751..>18196)
                     /locus_tag="PKM23_02995"
     CDS             complement(17751..>18196)
                     /locus_tag="PKM23_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp/Glu/hydantoin racemase"
                     /protein_id="HNY90471.1"
                     /translation="ILGCTHYPFYLDRFRAELDRLRGYRENGEYIYQPFMADTIHLVD
                     PAINTARELYDHLRRQGLFAERDLAGSEFFISVPNRSRPEAELDAAGNFTWAYKYGRR
                     AGMGQEYVKYVPFSREVLPAESIQRLERTIPRTSRLIWKENLPGK"
BASE COUNT         3583 a         5155 c         5986 g         3472 t
ORIGIN      
        1 cgccgttgac cgctgcggcc tgcactgtga agagggtgtc gaccagcccg agggtcatgg
       61 tcacgctggc cttgccgttc tcgtccgagg tggagctgct gcgggcgagg gcggcaccga
      121 ctccggaaat caggctccag ttgacggtgg ttccgggcac gccgttaccg taggcatcct
      181 ggatgacggc ggtcagggtg gccccggcgg tctcatcgcg tgtgccgagg atgggatcgg
      241 tgagatcggg atccttgatc atcgagaccg cgggccccga cgtgggatcg agggtgaaat
      301 tcaccggcgc cccgttgagc agggtcgtgc cgtcggcccg gtaggcgatg gcggagacga
      361 cgacattggt gtcggcatcc gtgtcgaatt tcaggcgcgc ggcggcgaag ccggcggcat
      421 tgctgatatt ggagagattg gggccggtat cccccatggt cgagccggtg ggaccactca
      481 ggccggtgct gatgagggcg atcttgtccc agtcccagcc cggatcataa atcaccagac
      541 ggatgttctg cagtcctttg ggcagcctga agctggtgtt gtggcgctgc cagacccagg
      601 agccgctgag gtcagtgaaa tccatggtga gcgaatcccg gtcgctgtac ttgatgatca
      661 cggtattttt ctgaccagag gtggtgcggg cgcgcagatg gaaatagtag gtacgctcct
      721 ccggattgta gatggggatg gtcaccgccg tccggaagcg atagccgccg atataggtgg
      781 ttttgatggc atgaccaccg gaggcggccg cggccgtggt atcataggcg atcgggacgc
      841 cgaggatgcc cgactcgacc tctttccaga tcttgccgtc gaaggtgccg gtctcgatgg
      901 tgcccttgcc ctgggtgacc gcaaaatcga cgacgacatt gctgaccgga ttgcctgcgt
      961 cgtcggtcac cttgaccttg agggaatcgg cgacgtactg attgatgggg gccgactggt
     1021 tgttaccgct ggcgtaggca atggtggtgg cgctggcgga gccgaggttg ggcagataga
     1081 ccgagaaatt gtcgagattg aaattgctcg aaccatcttc atactggacc gcgccgacgt
     1141 accaggaatc gggaggggct gcgggcaggg tggtgttgag ggtggcgtca aaaacatcat
     1201 cgatatagtg atggatggtc agggccgtcc cggtcgcctt gatgatggcg gtgaccttct
     1261 ggccggctgc aggagcggtc cgggtcacat tgacttcacc aatcagggaa ttgaaagtcg
     1321 agccgccgct gatagcgtag agggagagct tggtggcgga gcggcgcagc cagtagccat
     1381 tggctgaagc gctgggagtg tccagaatca gggcaaagcc ggccggggtg tagccctggg
     1441 tctggtaata gatggcggag gcgcccatgg tcatcgacag cttgatcgaa tcggccccgg
     1501 ccgacttgtt ggcgccggtg cgtgcgaagg cggccagacc gtagccgttg gaggaggcga
     1561 tggccagttc gttgttctcg atgacgaatt tgggggcatt ccagtcgctg cccaaagagg
     1621 cgcggttgaa atcatccaca atcgggctgt tccagccggc cgatggcgtc cctccgccgc
     1681 caccgccgct gccggccgtc gtggtcgccg aggcgacgtt ggagagcggc gagggattgt
     1741 tcgcttcgtc gtagatcttg agagcaaaat agtacctggt gctggcgttc agtccggtca
     1801 ccgtatagct ctcgggagag ccgggcgcct tgggggtggt ggtagtgaag cgcgttgaag
     1861 aggaaaaatt ggcgtcggtg atcgccacgg tgttgtagcg cagctggtag gtggtggccg
     1921 cgctgccggt ggcgccgtca tcggcggtgg cgttccaggt caccgtcacc gagccggcgg
     1981 tcgggctgcc agcgctgatg gtagccgcgg cagggggggt ggtatcggtg ctcgtcgagg
     2041 gggtgtaggt gctgaaggtc atctcctcga tatcattatt caccaattcg ccgctgtaga
     2101 gcagggcgcc gccgtaacag gctgcggcga gattgtaatc ggtattggtg atcgtgccga
     2161 gcgaaacccc gtccaggaaa tactcgaatt tgccggttgc cgtattgacc tgaacagata
     2221 agagggaacc ggcgttcatg gtcggcgcgg atgcgaccgg aacggaggtt ccggaggtgg
     2281 ccactgcgcc ggccgtaatc ttgaaaagcc tgatttgccc gctgtaatag ttggcaaagt
     2341 agccgttgcc ctggtaattg ccagtctgca taaagacgac accgccgtaa ccggtataag
     2401 cggtctcgca cccttccccg acgggcgccc aacggagatc gaccttattg gcatcggact
     2461 tgccaatata catcgcgatc tgtttggtcc ctgtcagctt tttggtcgaa tgcagattgc
     2521 cgttatagat cagggaatcc ccggcagacc aggaagaagc gggaagcgtg ccggtgccct
     2581 cgaaataggc agggacggaa tcctgtcccc aggccatgaa ggcgagagag agcgacaaga
     2641 gcacaaccag agttgcggct tttgagaacg aaaacgcgat tctcatgatg tgtaacctcc
     2701 aatgcaggta cggtacaggg tgctgtttca tatcagaaca cttgtttgcg cctaaaacag
     2761 gaaaatgccc cttttggcaa gaaatttcga gtcttacttc aatagaatta acaatgttgg
     2821 tttctcacca ccatctcatt tcgcaaacac tatgccaaaa tgcagagggg gcgaagaatg
     2881 ctttgctgcg ggacagggtg aaggaggcgg gcctgcagcc ggcctggggg agcgggccga
     2941 ccaccgggct gcggaggcag cccggcataa gcgggtaaaa gcggcaggcc tgcagccgta
     3001 ctaacggacc agctgcagac cttccggacg gtggggcggg atcacgtcag gggtgacccg
     3061 cttgagataa cggaaacgca tggcgtcagc ggcgacatag ccgctgccct gattggagat
     3121 gcgcacatag ctggaggtgc cctcgggcag atagaggatg gcgatgcggt tccagcgccc
     3181 gtagctggtg gtctggtcga tgacaccggt cagctttttg actcccccgg ccacggcttc
     3241 aaaggggaca tccgccgcct cctgataagc ggagggggtc tcccctacca tggggtgcca
     3301 ctcgctgatc tcgtaccagc cggccacccc gatggtcggc cggaaggtgg cccagccctt
     3361 gcccagcgcc ggagcggagt agtaatagcc gatgccggtc gagtcggagg agacccactg
     3421 cgagaagcag cggttacgcg aggcgaaatc gactgtctcg ttggcccggt cataaaccgg
     3481 ccggaagacg ggatcgaacc gggcgcgggc gctgccgggg gtggtatcat tgaagtagca
     3541 ggtgccgacg atgatgtcgg ccaccacggt gtctggacgg gagagcagga tgatgccgtc
     3601 gccgatattg ttcttggcgg gaatcaggga ttccccctgc agttcgacgc tgccgaaggg
     3661 ttggccgttg ttgaaggagg gcttttggcc gccaagaaaa cggtaatagg acccctgata
     3721 gccgggcagg ccggagatca tggcgtcgga gatgttgacg atctggccgg aggcgttgca
     3781 gatggccgcc cccttgtcga aaaagcggac gtagaccccc ccctggcccg ggatttcgcg
     3841 cgcagggccg accggatagc cgagatcgac atcgaactcg tcgtaccaga gcgacatgct
     3901 gtattcattg ccgcaggtca tgccgtaata ggcgcccgac atcagggcgg cggtaagacc
     3961 gtagcggatc ttcttatagt cccggctgtg ggtctccttc tcgacgtaat cctcgatgat
     4021 ggtgaaaacg gtgtcgtggg cggcctccca gagctggttg acccggatca gatcgggggc
     4081 ggtgtaggcg aagcgcataa agccctccca ctccatgccg tcgcagtagc gcaggggata
     4141 gggatcgccc tcctgggcgg agcccatgtt aatgccgacc acgaccggtt tgcccgggtg
     4201 gagagcggtg aatttggccc gttcataggg gagcatttcc ctgatgcccg cgctccactg
     4261 ttcattgaac caggccagcc ccttatccgg ctcggtgagg tcgttgaggt ggttatggtc
     4321 gagatccagg gtcgactgaa agtcgtcgcc ccagaaaaag aagcggaagg agtcataaaa
     4381 gaggccgttg aagcggctga aatcctgttt ggccggattg aagcgctggt cgatatacca
     4441 tgcccagacc ggctggctgt tgaccagggg ggccaggggg gtgacgttgt ggagatagcc
     4501 gaagccttga aagcgcgccg gccgcttgat tgaatctccg gcggcgtggg agcggttgat
     4561 ggcgtaatcc ccgctgacgg ggatgccgtg gatggtcgag tcggtgtagc cggtgaagga
     4621 gatccagtca tccccgccga tcagggcgta gatatcatcg ccgatggtgt tggtcttgaa
     4681 cccggtcgtg gaatcgacgc ggatatcggt gtcgcccggt tccgccgcct cgatgagaga
     4741 gccatagtag ctggagtaga taaagcaggc cggcggccag atcggattgc cgggccagac
     4801 cccctgtctg gaggtgccga ggatgacggt gtttggatta agggcgtgga tgctgtcgtt
     4861 gagggcatag gcattaaggc cggcgccgcc gtggatggcg agatcatagc ggctgaaaaa
     4921 atactgcaca gcccctcccg agatgccgcc gggccgctga aagaccagac ggggaaaggg
     4981 gggctgatac tgctgcgccg aaaggggtgg gaccaggctg agaattaaca gcattaaaag
     5041 ggcgatgaac cggccggccg gccggtggag cacaagggac atcggtttct cctctcaggc
     5101 tttgcaaagg gtacaaggga tggaatgcct ccaatccata atacccgcag ggccgaggga
     5161 gtgcagaaaa tgaggagaaa cctggggatc actctcccgt ggccctgacc acctcgggga
     5221 tggtgcgcag catgatcttg aggtcgagcc agatcgacca ttcgtcgata tacttgagat
     5281 cgagtcgcat ccattcgttg aagcgcaggc ggtggcggcc gctgatctgc cagaggcagg
     5341 tcaggccggg gcgcatgctg aagcggcgga tcggccactg ttcatagatg ttgttgacgt
     5401 cgcgcaccgg caggggccgc ggtccgacca ggctcatttc gcccttgagg acgttgatca
     5461 gctgcgggat ctcgtcgatg ctggtccggc gcagccagcg cccgaccggg gtgatgcgcg
     5521 gatcggcagc gattttaaaa gccgcgccat cggcttcgtt gaggtgctcg agctgcgcct
     5581 ggagggcctc cgccccgtcg accatggtgc ggaacttgta aatcctgaag cggcggcggt
     5641 tgaatcccac ccgctgctgc ttgaaaaaga ccgggccgga tgaggagagg gcgataaggg
     5701 cgctgacgac gagaaagagc ggggcgagca gaagcagccc cgcgccggcc agggtgacgt
     5761 cgagcagacg cttgatgacc agatgggaaa agcggcccct gccggcgttg cggagggtga
     5821 gcatcggcac gccattgagt tcataagccg gagtatcggc cgttttcagc tccatccagt
     5881 cggaagggac gcgacagggg atggagagct catcgcaaaa gcaggtgatg cggcggacct
     5941 gctcgtagca ggtgctgatc ggcagggcga taaagacctc atccactgcg tggcggcaga
     6001 gatagtccgg aaaggcatcc agggtgcaga gccacttgcc ctgccagagg cgggtgttct
     6061 gggggtgatc gtcgacaaag ccgagcagcc ggtagccaag gtcggcgcga tgaaggatct
     6121 tttgcgagag gtcgaccgcc tcgggtccgc tgccgacgaa aacgatatgg cgcaggttgc
     6181 ggccgcggct gcgcaggaaa accagtccct ccctcaccag ggcgcggctg gagatggagg
     6241 cgaagagggc aagggcgtaa aagaggagga tgaacttgtt gtcgagctct tcggccacca
     6301 gcaatgtgag ggccgcggcc acgagggtgc ccaggctgac cgccttggcg atgtcgagcc
     6361 actccctgac gcgctcatcg agacggcgca ccgtatagag gccgaaatgg gcgaagagac
     6421 gattccaggc gatgagaagc aggatcaggg ccagaccatc gaccggccgc aggagggccg
     6481 gtgtgaactg gctgacaccc cgcaccatcg agagctgtgc atgcagcagc acagccccgc
     6541 tcagagagca gagcatcacg gtaagatcga gaattcgggt tcccttcgcc agcagctttt
     6601 tccgggtagt gcccatacaa atcccttgtt tcaacaggat tggcgaaatg gcatcgaatg
     6661 agtggagagc agacgccggt actcgaggaa aggatagcaa tccgcatgcc agaaagcatg
     6721 ggtaagagag gcgggaaaac gcagaaataa aacaatgagt tacgtaattg taatccaaag
     6781 agagcggtgt cgcagacgcg cacgggcgtt cattttagcg acattgtcat ccttttttgc
     6841 ctaccggggg accaccgaat cggtttcgct gtaggcaagc ccgtactttt tcatcttgtt
     6901 gtagagggtg ccgcggctga tgcccagggc ggtggcggtg cggctgacgt tgccgttgta
     6961 cttgagcagg gccttgcgga tgagcacctg ttcgacgtcg tcgagctgca tggagcggac
     7021 gccgagctgg aggggctggt tttccggcac cggttccagt ccggccacct gcagggcgtt
     7081 ggattcgatc tgcatggcgc ggctcatgat cgccgcccgc tgcaggatgg tgttgagttc
     7141 gcgcacattt ccggcgaggg cattgcccct cagcatggtg agggcctccg gctgcagctc
     7201 ccgctcgggc agggccagat cttcgctgat ctggcgcagc agcacctgac aaaggcgggg
     7261 caggtcggcc tggcgctgca tgagattggg caggtggacc aggctggcgc cgatgcggtt
     7321 gaaaaggttt tccgccgcct cgtcgggggc ctctgtgctc aggccgatga agcggatgcc
     7381 ggagcccggc acagcatggt gctgttcctc gatgaaatgg gcaaggcggt tctggacgga
     7441 aaccggcagg gcatggctct gttcgagcag cagggtggcg tcataggagg gttgcagcag
     7501 gccgccgcgg gtgccgaaga gttcgagcag gaggtcgccg gaggtcttgt gggcgcaggc
     7561 gacgcgcaac aggggctgga agcggcgcgg gctgaggaaa tggagcaaac gagcgaagta
     7621 ggtcttgccc accccctcct ctccgcagaa gaggatatgg ctctccttgc gggccagttc
     7681 gccgaccagc tgcagggtgc ggagcatctc ctcgccgctg ccgacaacgg ggccgaggtc
     7741 gcgttcgcgc cgccagccca gccgctgggc gatgcggaag tggggggaga ggtcgagctg
     7801 catggcgcgc tcgagggcga gggggacctc ctcgctgagg atcggctgca ggagatagtc
     7861 ggtggcgccg agccggatcg cctgcatggc ggcgttgagg tcggccgggg cgacgatggc
     7921 gatgaggggt ttggtcggga aatggttgac gaattcctgc accatctcaa agtggcgttt
     7981 gtctgcgctg atgtcgacaa tggccgcatc gaatgcggcg atctgctgca ggcagtagcg
     8041 gaattccgct tcggtggtac tgcgcaccag gcgtccgtta gccgcggcga ggctggccgc
     8101 tatggtgttc atggtctttt cgctggtgga gaatacaaaa aaccgtctta aatccgccat
     8161 ggcaggttcc ctttgcgatt ctcgtccttg actgttgtcg ttttttgatg gaagcaggga
     8221 aaaacaaatg caaaaacagg gccagttacc gggccgacag cgcggccgtc cggtatgtcc
     8281 caactaattg atttcatgca gagaggattc accccgtccg cgatgcagcg ggcggattcc
     8341 tgtcttgagc tgcaacaatg ttcatgaata aaacaccagc ccgaaagccg gctcagcggg
     8401 ggcttttctg atggtgatcc ggcacccgcc gcagcagcca ggcgccgacg gccatgttgc
     8461 aggcgagggc ggcgatggcc gccttgtatt taatgaccgg cccgaaagag gccagggacc
     8521 aggtgatggt gctccacagg atcgggccga ggacagccgc tactttaccg gagagggcgt
     8581 agaggccgaa gaactctccg agtttctccc tcggcaccag ggagatgagc aggggccggg
     8641 ccgaagtcca ggtcgagccc atgagcatgc cgatgatgca acctgcaatc cagaagaggg
     8701 tgcggctgcc ggtggcgatc accccggtca gggtcacaac ccagagcaga atgacccagg
     8761 tcagggtctt tttgggaccg aagtgatcgg tgaggatacc gcagagggcg gagccgatca
     8821 ccgccgaggg aatgacgatg acgaagaact ggttcgcctc ggccagggtg aatcccatca
     8881 ctgcctgggt atagatgccc atgtagatga tgacggtttc gatgctgtct tcatagagca
     8941 gcttggcgag cagaaagcgt ggcaggccgg ggtagcgccg ggaatcgacc agcgaccgga
     9001 aggtcttgcg gtaggattcc ctcaggctcc atttcggccg gcccggaagc gcggcatagg
     9061 gctctctgac aaaaatgaaa atgggtatgg caaagaacaa aaagagcagt gcggtcggta
     9121 taaaggcggc ggcggtgcct cccccggtca ggccggggat cctcaggccg taaaagtggc
     9181 cgtcgacgaa aaggccggcc acggcgaggc cgaggatgga gcccatataa ccgatcgcga
     9241 cgccgtagcc cgaaacccgg ccgatggtgc gcggggtgct cacggcgggg agcagggcgt
     9301 tatagaagac cagtccgccc tggtagctgt aattggcgat gacaaagacc gccatgacca
     9361 ggggcagaag aatatgcaga tcggtgatat ggttgccgag aagtcccagc actgccgtcc
     9421 caaggatgca ggcccctgtg aagaggccga ggagagccat ttttccgccg cggtggtccg
     9481 accagtcgcc gagcaccggc atggtcaggg ccaccaggat catggagaag gagttgacaa
     9541 aagagacgat gaaatcgctc tggccgaggt tgataatgat ccaggtgccg aagtagagcg
     9601 aaacaacatt catcgaatag gccgtattgg ccaggtcgta gagaatccag gagatggtgg
     9661 ccgccagcct gggccggctg ctccgctgca ctcctgaaga atccggcccg ctcgcctccg
     9721 aggaggtcac tgcgccgcct gcgcttcgat cagccggcag agccggttgg ccagaccgcg
     9781 atagctcctc tgcgccggaa ccacgatcag cagcagggcg gtctgcccga aaagggtgag
     9841 gtagcggaag acgagcctcc tgccgtcgcc atcctcaagc acaagttcct tgagggggct
     9901 gaggccgctc atctttttga tcaccttttt aaacttggcc atcaacaccg cgaactcaac
     9961 ggcgtgatcc tcccggagca tgcttgagtt ggccacctgg gaggcgagca ggagtccctc
    10021 atgggtgaag agatagatcg ccgcaaatcc gccgatctgg accatcccct gcatctcgcg
    10081 ccgggccgct tcctggaagg gcagggcctc ctcgttatcc gctctgttca ggccgaagtg
    10141 agccattctt gatccatcat ttacatttta ttcagcagga gctgaatcac gccgcgcagg
    10201 gtttcgaaga cggcgaggcc ctggacagcg acggcctcgt aatggggaac cctgagaaaa
    10261 ttcaggcttt gttccatctc gtccacggag tcgatctccg tcagatcgcg tttgttgtac
    10321 tgcaggaccc aggggaactg ggccagggtc ttgccctgct cgatcagctt cagctccagg
    10381 tccatcaggg aatcgagatt gtcctgacgg cgggctgact gcgagtcggc gacgaagacg
    10441 atggcgtcga cgccgcgcag gacgatcttg cggctcgatg cgtagtagag ctgccccggg
    10501 atggtgtaga ggtggaagcg gggctttttg cccttgatgg ccccgagcgc gagttcgaaa
    10561 aaatcgaaat agagggtgcg ctcttcgggt gatttcagca ccaccagccg gctgcgcaga
    10621 gccgggtcga ggtgggcgtg cagatattcg agattggtcg tttttccacc caggccggga
    10681 ccgtagtaga ctatcttgaa aacgatctcc tgcctgaacc agtcaatatg catatcatct
    10741 tggccgcagc gagcggtcga gctgccgcgc gagcatgtcg cggaattcga aatcgaggaa
    10801 ctgatgggtt tgttcgcccc cctggcgcag ctcctggatg tagcggccga ttttttcgac
    10861 cgtataatgg gagaggacgc ggatgatgcc gagggcgacc gatttgtcaa agaccaccat
    10921 aagaaaatag tcggtgccga ccgcacagag atagaggctg cgggaggccc cctcatggta
    10981 gagaaacttg aagcgtgact cctggccgat cagccgcgac atttcagcgg tggcggcaaa
    11041 gtcgccggcg gccagggcgg tgagggcggc cagatcgagg gtgtcggcat cgccgcgata
    11101 gaccaccgga tatccgctca tgtcggcgaa aagggccagg cgcgcctgtg aacggctgag
    11161 aaactcctcg aggatgaggg cgatctggtg ataggcggtc tggctgagaa ccacccttct
    11221 gccattcttg cgttgctgca tttccatagc acaaccagtc cgttaatgac caaactgaag
    11281 cctcagggcg agcatttcgg gcagacggcg cgtgcgcatc agctgctgcg tcttctgcac
    11341 atcatattcg accctgtgaa attcgactat gccggtctgt tgatcataaa gggcgaaaca
    11401 ggcgcgcgga tcgccatcgc ggggctgacc cacactgccg acattgatga tatagcggtt
    11461 cctcgggttg agcgtaatgc ggccatgccc gggaagggga taaaatcctc cctccgcccc
    11521 ggaggcaaat ccgcccgggg tgtgagagtg gccgatgaag cagatcctct ccgtaaaagc
    11581 ggcgaagttg cgctccgtat ccgtgatatc gtgcaaatac tcccaggcag ggggattgac
    11641 aggcgttgcg tgtacaaaga agatgccatc acgtcgtccg acgagctcgt aacgcccgag
    11701 tccggcgcgc gtctcttgag tgatcacccg tccggtccag aggaccgccc gaagggccaa
    11761 gtcgttgaaa aagaagggat tggttcgccc caaaactgcg tcatcgtgat tgcccagaat
    11821 ggcgagcgaa gccacttcgg cgatggcagc cacacattca tccggctgtg ggccatagcc
    11881 cacagcatct cccaggaaca caatctcatc acagtgctcc gctttcagcg tgcgcagcac
    11941 ctgctcgagg gcctccagat tcccatgcac atcggaaaaa atgcccacgc gcatagcaac
    12001 tcctcagcag ttagtagaat atagcatttg cccgcgcggt aatcaagcat aattttggct
    12061 tttttattgc ttctttcggt cagttgtgtt agatttgagc ggatcagcaa gcccaacaag
    12121 gagaccccct atgtccagtc ccatttccca gctggccgaa tcgatccgca tggccatcga
    12181 tctcgaaaaa tcgggccgtg ccttttacct gctggcagca gagagaacgg ccaatcccac
    12241 cggcaggacc atcttttccc gcctcgccaa ggaggagacc cagcatctcc tcaccttcga
    12301 gcgcatgctg gatgatgaag agggtctttc cggctggcgc tcctggctga aggaataccc
    12361 caaccatccc cccctgccgg tcttcggcga aaaggccgaa aaatcgatca aggagggctc
    12421 gatggatgaa ctccaggccc tgcgcgtggc gatgaaacag gagcgcgagg ccatggagta
    12481 ttacggccgc atcgcggaga gcagcgacaa cgaggcggtc gccgggatct tttcctttgt
    12541 gcgcgagcag gaggtctatc accacgacct cctccaggct gaatacgact cgatcaccca
    12601 gtccggcttc tggttcgata acgccgaatt ccgcatggac ggcaaattct gatcaccggc
    12661 caccgggggg cgcacgcaag gcaacgaagg atactctgat ttcatggcgg acaagactcc
    12721 cctcattctc gacccttttc aacatgacgc ggcggtgcgc cactttctcg gccatttcgg
    12781 gctggcgccc cgcacgccgg accgtccctt cctcgaaaga atcctcgcca gcttcgcccg
    12841 gctcccctac gagaacatca gcaagatcat caagctgaac cgccattttc tcgacatcga
    12901 acggatccgc ctgcccgagg aggtgatgga ggggtatgtg cgccacaacc tcggcggcac
    12961 ctgcttttcc ctctcctact ttctgcagag catcctcctc cagctcggct tcagctcctg
    13021 gatcttcatg gccgacatgg gggcgcgccc caacgtccac tgcgccctcc tcgtccagct
    13081 cgagggcagc aggctgctcg ccgatcccgg ctatctgctc agccgcgcca tggagatcca
    13141 ccccgaccgt tcgcgcctct acgaaacccc gcacaccggc gtggagatca aattcgaccg
    13201 cacccgcgag cgttacggcc tttacacctt tgaccggcag gtgaaaaagc tgcgctatac
    13261 cttcagcgac cgtccgatac cgccggcgga gtttctccag cactggctcg cctcattcta
    13321 ccagggcatg atgcacaaca tctgcctgac ccagctgcgg ccggagggat tgatctacat
    13381 gcacggggat tacctgcaga tcgccacccc ggaggggcgg cacaagcgcc gtctcaagca
    13441 ggagtatccc cgggtggtct cggaactttt cgccatcgcg ccggagtgga tcgagcgagc
    13501 gcagctcgcc ctggtcgaga atatggatat ggaaaagcgg cacgggatct accggcccac
    13561 atccggggct gagagccggg aggaggggtg atggaagccg ctgcgccgac accggtcaaa
    13621 ctcatctgcg gagtgctcta cagcgacgat gctctctgcg aggaggccct ggggctgctg
    13681 gcggagcgct acggccgcat cgactaccgc tccgccctct tccccttcac catcaccgac
    13741 tattacagcg ccgagatggg cgctccgatc caacgcttct tttgcacttt tgaacccctt
    13801 atcgatcccg gcaagctggc ggggatcaag atcgcctgca acgaaatgga ggccaagctg
    13861 acggtggacg gcggacgccg cgtcaacctc gaccccggct acatggatta cgacaaggtg
    13921 gttttggcct cggccaagta caaaccgcac aaaatctatc ttgatctcgg catttatgcc
    13981 gacgccaccc ttcattatga aaaggggcac taccatcccg cgccctgcgc ctttcccgat
    14041 ttcaaatcgg gtgagtataa tgaaaccttt ctcgccatcc gtgcccgtta caaggggcag
    14101 ctgcgcaagc tgctggcggg gcgaatccgg gagtaagagt cgtcgccgcc agagccccgg
    14161 gcgggttccg cggcgcctgt gtccgccggc tgctaaatga aaagggcctg cacggttgcg
    14221 tgcaggccct tttgcatgac agccctctgc agtgaggcgc cggcgatcag agcagcagca
    14281 gggcgggatt ttcgagaatg gccttgaact ccttgaggaa gagggccccg gtggcgccgt
    14341 cgacgatgcg gtgatcgcag gagagggtca ccttcatgcg ctggccgatg gcgatggcgc
    14401 cctcccggac caccggcttg gcgatgatgg cgcccaccgc caggatcgcc gcctcggggg
    14461 gattgatgat ggcgctgaac tcctcgatgt cgaacatgcc gagattgctg atggtgaagg
    14521 tgctgccggt gtattcgtcg ggggtcagtt tgcgcgcctt ggccttgtcg atcagcaccc
    14581 tggcctcggc ggcgatgcgg ccgacacttt tggcggcggc attgcgcacc accggggtga
    14641 tgagcccatc ctcgagggcc acggcgaggc cgatatcgac ctggctgtgg aagaggaatt
    14701 tgtctccggc gaaagagccg ttgacccggg gatgtttgac cagggccgcc gcagccgcct
    14761 tgacgatgag atcgttatag ctgatcttga tctcctcctg ggcggcattc agttcggtgc
    14821 gcagggcgat catcctctcc atatcgatct ccatggtgag atagaaatgg ggcacggtgg
    14881 tcttgctctg ggtcatgcgc gtggcgatgg cggtgcgcat ggtgttgagc ggttgttcgc
    14941 tgtcggggcg cgcagcttct ccgggggtga actcggtcag ggatagcgcg gccggcgcct
    15001 ttgcagactg gatggcagcc gccggcgcac ttgccggcat ggccgccaga tcgcggctga
    15061 tgatgcgccc gccagtgccg ctgccctgca cccgccgcag atcgactccc ttggccgcgg
    15121 ccatcttgcg cgccagggga gaggccttga tccgcaccgt atcggtagtg atgatgacat
    15181 cagccggggt gacatcgggc accggggcgg cggcgatttg ttcctccgcg gcgggcccgg
    15241 cgggggcttc cgcctcaacg ggtgcggagg gaacggctgc agcttcggat ccggaggcca
    15301 gcagcgcgga aatatcctcg cccttttcgc cgatcaccgc cagcagtccg ccgaccggca
    15361 ccttaccgcc atcggggacg acaatcttga gcagggtgcc ggcatcgaag gcctccagct
    15421 ccatggtcgc cttgtcgctc tccgcctcgg ccaccacctc gcccgcctcc accgcatcgc
    15481 cttcccgttt atgccatttc agcagcacac ccagggtcat ggtgtcgctc agcttcggca
    15541 tggtgagttt gcttgccata tagttccttt tctgtggtta gacagatcct gacaaaatta
    15601 agctctcacc aagtcacgaa gacacaaagt aaaaccacct gcagatacag ttactggcta
    15661 tcagcctttt ctttctttgt ggcttggtgc cttggtgtga gccaataccc gttttaaacg
    15721 tagagcacct ttttggcggc ggcggcgacc ttctgggcgc tcggcaccac cgcgtgttcg
    15781 aggttggccg cgtagggcat cggcacatcc tcgctggtga tgcgcaccac cggggcatcg
    15841 aggtggtcga aggcattaag ggtgatgcgg taggcgatct cggcgcccac gccggcatag
    15901 ggccagccct cctcgacgat gacgcagcgg ttggtctttt cgaccgagct gatcaggagc
    15961 tgctcgtcga gaggccgcag ggtgcgcgga tcgaccacct cggcctcgat tccctcgccg
    16021 gcgaggatat tggcggcctc gagggcgacg tggatcatct tggaccaggc gatcagggtc
    16081 acatccttgc cgggccgctt gatatcgccg acgccgatgg gcacgatgtg ttcgccctcc
    16141 ggcacctcgc ccttgtagcc gtaggccacc tcgctctcga aaaaggcgac gggattgtca
    16201 tcccggatgg ccgccttgag cattcccttg ccatcggccg ggacgctcgg ggccatgacg
    16261 atcagaccgg ggatatggca gagccaggcc tcgagtgact ggctgtgctg ggcgccgagc
    16321 tgatgggcgg acccgttggc cccgcgaaag acgataggca tcttgatctg gccgccggac
    16381 atgtagcgca gcttggccgc gttatttaca atctggtcga gcgccagcag ggagaaattc
    16441 caggtcatga attcgatgat cgggcgcagc cccacctggg cggcgccaac gccgaggccg
    16501 gcgaaaccca gctcggcgat cggggtatcg atgacccgct gcggtccgaa tttttccagc
    16561 agcccctggc tgaccttgta ggccccctga taataaccca cctcttcgcc catgaggaag
    16621 acgttgggat cgcgctccat ctcctcggcc atggcctggt tgagggcttc acgaaatgtt
    16681 agttctggca tactctgctc tccaggtaag gttgacaggg aggttcagga atagatttca
    16741 gtatagagct cttccagggc cggcaggggg ctcttttcgg caaagtcgac ggcgtcaagg
    16801 acgatctttt tgatctcatc ctcgtattct ttcacctttt ccggggtgag aatcttgtca
    16861 tcgatcagct tctgctgaaa gatgaggatg ggatcctgcc gcttctgctc ctccacctcc
    16921 tccttggtgc ggtaatggga gtggaccgga tcggacatcg agtggccgcg gaagcggtag
    16981 gtgcgggctt cgatcagggt cggttcgccg tcgcgggtgg cgcggacaat ggcctcggcg
    17041 acgacgtcgt gcacggcgag cacatccata ccgtcgacgg tggcgcgggc catgtcgtag
    17101 gagcaggcct tctccgagag gtcccaaacc gcggaggcgc gctcgagggg ggtgcccatg
    17161 ccgtagcggt tgttctcgat catatagatc accggcagac gccagagctt ggcgaggttg
    17221 agggattcgt gaaaggcccc ctgatggacc gtgccctcgc ccatggagca caatacgacc
    17281 ttgtcttcct tgcggtactt ggcggcgaag gccatgccgg tggccaccgg cacctggccg
    17341 gcgacgatgc cgtggccgcc gtagtagcct ttttcgacat cgaagaggtg catcgagccc
    17401 cccttgcccc tggatacccc ggtggccttg ccgaagagtt cggccatgac cgcgttgggc
    17461 tccccgccca tggcgaggat atggccgtgg tcgcggtagg ccgagatgaa ctggtcaccg
    17521 ggtctcagag ccgaaaccgc gccgaccccg accgcctcct ggccgatata gaggtgacag
    17581 aaaccgccga tcttttgcat gccgtacatc tgcgccgtgc gctcctcgaa acggcggatg
    17641 agcagcatca tgcggtacat ctcaagcagt tttttctttt ccatggttac cctctgctgg
    17701 taggaaaaag cgcatataaa ttcaaaagta atacaattat tgccgcgtta ttatttcccg
    17761 ggcagatttt ctttccagat cagccggctg gtgcgcggga tggtgcgttc gagccgctga
    17821 atgctctccg ccggcagaac ctccctgctg aaggggacgt atttgacgta ctcctgcccc
    17881 atcccggcgc ggcggccgta cttgtaagcc caggtgaaat tgccggcggc atcgagctcg
    17941 gcctcggggc ggctgcggtt ggggacgctg atgaagaact cactcccggc gagatcccgc
    18001 tccgcaaaga gcccctgtct gcgcagatgg tcgtagagtt cacgggcggt attaatggcg
    18061 ggatcgacca ggtggatggt atcggccatg aaaggttggt agatgtattc accgttttcc
    18121 cggtaacccc gcagccggtc gagttcagcc ctgaagcgat cgagatagaa ggggtaatgg
    18181 gtgcagccga ggatga
//
LOCUS       DAOPWU010000028        17862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3212_length_17862_cov_5.354636, whole genome shotgun sequence.
ACCESSION   DAOPWU010000028 DAOPWU010000000
VERSION     DAOPWU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17862
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3212_length_17862_cov_5.354636"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PKM23_03000"
     CDS             complement(<1..668)
                     /locus_tag="PKM23_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNY90472.1"
                     /translation="MDTRITLPRRPAKPIMAVKTLVLVGLALLAGFLPSPAAGQPLTQ
                     GLDRFLGCSTSSEYTRYMVKYWNQVTPGNDGKWGSVQPAQNYFNWSNLDKIYNYVTSK
                     GLLYKHHCLVWGQQQPGWITALDQPAQRAAVENWIKLVGERYPKMDYVDVVNEPIRAP
                     AEYRAALGGDGATGWDWVITSFELARKYCAPGTKLLINEYNVLQDNAITSQYISLINI
                     LVSRG"
     gene            complement(745..2631)
                     /locus_tag="PKM23_03005"
     CDS             complement(745..2631)
                     /locus_tag="PKM23_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNY90473.1"
                     /translation="MKTDSYAHETRRPGRLCAAAVLALLWCTLHFPAAALAQPSGGPY
                     GPVRQNYALPAGAARILYVAADAPAGASGASLAEPMPLEEAVAAARSGDAIILRGGLY
                     RTGSLVLNQGVTLQPWADELPILKGSRVADQWTNLGNGLWTTSWERLFPEKPADWWRR
                     SREGKKTPLHRFNNDMVFADGSLLQAAGYEGEVDENSFFIDYDTKTVYIGVDPAEHLI
                     EITAHDCAILRTTKEIRGVKSDGRGYTLRGLTFSQYAYRALEIEGSDPEGISNESGHG
                     KMVVGTTIEDCTISFCSRVAGYFRGDGLTIRRCRISDTSTEGIYILSSNDVLLEKNII
                     TRNNIERITGYYPAAVKIFNQCYRVTCRDNLVTDMPWSNGIWYDVGNVDGVFVNNWIE
                     NVGSIETDIRRDQLWPSDNGFFFEISKGVICAGNLFVNCDHGLMILNSCDAQIYNNTF
                     VNSIACIGRNERSAQGDHFGWHPATGPGVDERDGHILVNNLFTATTGFERPLLFVWQP
                     PSLCDRLAEPMVERMDHNLFVRAPGQAKAPLLLWSPASTPTCQATLQSLEELKANHAE
                     FTGASLEYCDYEGPLFKSSELGHYQLLPGFGAARAGAQPPAAVRSAAGTREMRHIGAY
                     PPAR"
     gene            complement(2651..4366)
                     /locus_tag="PKM23_03010"
     CDS             complement(2651..4366)
                     /locus_tag="PKM23_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNY90474.1"
                     /translation="MKSNPLRRCPYLLCALLLLFAVGAFPQSDAIQTDVPALKEVYAG
                     DFYIGCLLSYPHVGFPSDPQVTGQTAVVAPNGGHLIKFHMNSMSPGNNMKPAYTVDIS
                     ASAAAYKAASIAKKDSIDTHPIVKFNGNLIAQLNWAQRQGFTFRGHTLVWHSQTPAEF
                     FRSGYSASGARLSKEKMIERLDNYIGSVIRLLHEGWPKLLNAMDVVNEAVTDAGEDRT
                     DNSEWYQTFGDNSYLLKAFQLTRKHTLALGESQIKLYYNDYNTHIPAKADGIVRICTP
                     IFQAGYLDGIGMQEHDSNSSPTAEQWIASYDKFYPICSEMAVTELDVTTGSATPSARL
                     LETQANQYGQLFKCFVERSYFSGRGKIIAVCKDGLNDEWTFKKNQSSSLWDTKNQCKP
                     AFYAVVDVGLSYNALDSLIAEAGTLQESLYTVDSWSVLTAALAKAQAVMTRSYANSLS
                     AADTLKTAKTGLTASLLALIPKTSAVALETGGSVTTFTLGQNYPNPFNPVTRIPYTLA
                     QEEEISLRVYTLTGQLAAVLFEGRRGAGSHLAEFDGRGLAGGIYIYTLRSGRSVESKR
                     FILLK"
     gene            complement(4502..>5610)
                     /locus_tag="PKM23_03015"
     CDS             complement(4502..>5610)
                     /locus_tag="PKM23_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90475.1"
                     /translation="FYAVEVDMGEPITVPEAPWEAYYVDSWGFYGGRLGGWKFIRGEF
                     DGDATASGDAPNTGWSALRGGFDAIAPTPEKALSITGKIELVGGGFEAVRSLRFALAY
                     SDSAGKVDTTSVDSTKWTGTDAHDFAYLFLPTSGANGPVDWQGIGAKGTLGAVVDNPW
                     LSTSGANNYVLGSVLQRPDQAVGGAGTYEFGISVQPLGNGTSEIRVKLLKTDGTYAWA
                     VKTTDMHSPLVTDKFNSILFALNTNASTTAMNLMEVLVDKGAPIELPDWVVSVEAPEQ
                     TAALPTVYALGQNYPNPFNPTTTMEVSLPKNSQVSLVVYDALGKVVAELMNGTYNAGV
                     HKVNFNAINLSSGIYFYKLTAGEFTSTRKLMLLK"
     assembly_gap    5611..5710
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5711..7385)
                     /locus_tag="PKM23_03020"
     CDS             complement(<5711..7385)
                     /locus_tag="PKM23_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90476.1"
                     /translation="MKKFAIVFLGVVAALLLGAVTLQAQIPKAIQTWDFETTLGSWMS
                     GGEKSAISLSAEQAAGGTQSIKLVKPATGLEINLQNDAYKDLKKGDQVSFKIWMSAAD
                     QAMVNGVQLFWQTGAGWSWNSIWINGSDITGDAWTTITREMANDVATPLQRIGVQVLL
                     KAGNEAATPAVYIDDFTIIRMVDITIVKDWGKTPQGTAWPILNTAATPDGDAGMGNNA
                     IPTGWATIRGQFSETMEATTEQAVVVYGQLEFVGGGGASNYTHLRYALTYQDSATLKN
                     QKTDSAAWVSTKKHFGYGFHPRTGTGTMSNGNGGAGVVWTIKDGSWSSTWSNNGLPIS
                     AAKQAPRDAVMEAGIYNFAISVQPLPDGNNEVRWYMVEQKNKYWYGGTTIDTAQVTTK
                     FNGICFGFNKDTEATQVNFYAVEVDMGEPITVPEAPWEPFYVSQWGKTTQGTAWPVKN
                     DSTYLDGDATLGNGAIPTGWATIRGAFGDPVTATLAKAVIVRGQMELVGGGGASNYTH
                     LRYALTYQDSSVLNYQYTDSAAWVSTKKHFGYGFHPRTGTGTMSNGNGGA"
     gene            complement(7600..9474)
                     /locus_tag="PKM23_03025"
     CDS             complement(7600..9474)
                     /locus_tag="PKM23_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90477.1"
                     /translation="MRRLIVFLICTMLLSVSLFAQQKDTTYVKGLYSGGVEGELNTVI
                     QDAVNSGSLNGKVFKLNPYDWYVLTGQIEVPIGQTLELVAPAPGTTQPSAPPQILWTA
                     SSSVTKNFIIAVYGDLIMKNIWVRYADAAGVQTGTPIVFDGDTTGVSGDTGEYGYFEG
                     CIFDYMPCPVVTASGSICVRSRHFNGTFKNCYFRNCVDAHFMYYGRAVSFPFDVPGYH
                     TDNISFENCTFANMGYVLMQEGTNYSDNVSFNHCTFYNVVQFPLESGWWWRLKVNNCL
                     FVNAWMMGYVPVQGVSGATVTIQQADSIAFSVPFTDQDRHILFTHSAYYLDNWLVDWM
                     RGGWDKDYSGTPWRPNPAINTVGNPYSKDEYKGRRFDAIPYPRPMLDSLALVYFDSTA
                     NGVKLYPFINRAELHDVNELREAINPGFIQPPLNLEPLKYFLWQKWSANLDSMWAYMP
                     EASFNHQWPLPENLAYTNETLKKAGMGGFPLGDLYNWWNPAVRDGSTDYYTAWKAQAD
                     AENARLATWMETGVDPTVGVHMVPGSALPKSYSLEQNYPNPFNPTTRIEYSVPVQGQV
                     SLKVYNMLGQEVMTLFNGVQAAGRYAANVDGARLAGGVYIYRLQAENMTITKKFVLTK
                     "
     gene            complement(9628..12591)
                     /locus_tag="PKM23_03030"
     CDS             complement(9628..12591)
                     /locus_tag="PKM23_03030"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNY90478.1"
                     /translation="MVLLPGLLLAGTGIIKGKVTDRDTKQGLPSANIIIKGTRIGTTA
                     DLDGNFTIPNAPTGQQTITVSYIGYATVNIPVTVKEDGVVRHDFALRAEALKGKEYVI
                     TAQAQGQVQAINQQLAESRISSVVSEAKIQELPDFNAAAAISRLPGVSTLESSGEANK
                     IVIRGLAPQFNSVTLEGVKLASTGSSQMGVSALGNTAGSVVNDRSVDLSMVSPYMIKS
                     ISVFKALTPDLDANAIGGTVNMELREAPSELHYDLLWQQGYTQKTGNAANYRAVGSIS
                     KRFFNDKLGVYALGNVEQYDRDADNMSASYNTASSKIDAASGYRPVRVTNTTLSRHIE
                     TRQRFGGNLILDYKLPSGSIKAVNMYTRLSSDYQDNNTVLNYSEKLLNFRYREGENNT
                     DISVNSLEFKYDFNWLQVDLKAANTASKNNLPESPFVELKTGPAITGSVPVNVIPDSL
                     KKLFRYPGDDQVYLDNINLFSSLYKENQTSFTPSFKLPFRLGSSLSGYFKLGGVYRMQ
                     TNSNDQKTPYASIRSSGAFQTALVNDLSNRFGIAVDPSTAKFAASNFTGNEHLMDPFL
                     DNQFGQIYWACDPTMLTAMARYLADSPQWRGKATGGADQTGGWYSGMFQTLANSYEYS
                     EDFYAGYLMSEIKFRNLMVIGGARYEESQSEYTAYNMFDMRNPDAQNCDTVIVNPRND
                     YWLPMVQAKYEPFRWFDVRYAYTHTLARPDYHQMSPKVTFDNPRKNIRSGNPDLKPAQ
                     AYNHDLTLTFHGNKLGLFSIGGFYKTIKEFTYYTTYILHPTSKSKDIKTISDFNIMGI
                     KPADGATLYTYINSPYDALVKGFELDFQTRLWYMPWGLDGIVLGINYTKIESEATYPL
                     RDEVTDYTTRPPVTTTFDSTRTGRLIYQPNDLLNAYVGYEYKGFSARVSCMFQGNSVS
                     YVGAFPEQDGYTRDYLRIDASARQTLPLAGSELYLDLTNINNRNNQSAQMSIDGFTNV
                     KNYGMTGNLGIRFRL"
     gene            complement(13057..14553)
                     /locus_tag="PKM23_03035"
     CDS             complement(13057..14553)
                     /locus_tag="PKM23_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY90479.1"
                     /translation="MNKEQIDRLPLAEKIGYGFADASANFVFQIMVMFQVGFYTDVFG
                     ITAAAAGTLLLVGRFWDAIFDPIMGSIADRTNTRWGKFRPWLLWTAIPFAVLFWLTFT
                     TPNFSPNGKLIYAYITYILLMTVYSMNNTPYSALNGVMTGDVNERTSLSSWRFIFAQS
                     ATILVQGFLLLMVASLGRGSAQQGWSRSIGILSLLSIVFFIITFLSVKERIKPAPGQK
                     ASFKEDVTGLLHNGPWMSMFLLTLFVFITLALWGSAMYYYFNYYISKEHLFSFLQSLG
                     MAAPAGAAGGEAVLSGWHKFLNAIGLLAKPDLSNASIIGFSLFNMVGTIISLTGIILS
                     KRLTLIFSKKGVFLVGLALTALFTALFYLLPREAVGWTFVLNILKSLAYSFTIPLLWA
                     MMGDVADYSEWKTARRSTGFIFAGIVFALKAGLGFGGAICGWLLSLYGYQPNVAQDGQ
                     ALAGIRMTASIYPAITFLIGVAALAFYGISKQMSLQIQDELAERRRSY"
     gene            complement(14606..16717)
                     /locus_tag="PKM23_03040"
     CDS             complement(14606..16717)
                     /locus_tag="PKM23_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNY90480.1"
                     /translation="MCHGMGQIARPSDLLGGLAPAALAEVTNRLQKYFIEQTRLGIPV
                     IFHEECLHGLAAKDATSYPQPIGLASTFNPELVQEIYSSIAADARSRGTHLALTPVID
                     VMREPRWGRAEETFGEDPYVSARMGIAAVKGFQGDGEYRDKTHLLATLKHFTGHGEPE
                     SGSNIGPANISERVLRDIFFYPFRQVVREAKPAAVMPSYNEIDGIPSHANVWLLQRVL
                     REEMGFTGMLVSDYCAITELFYKEDTVGYGLAENQLEAALLAARAGVNIELPDGDCYP
                     HLPELVRSGRLKESVIDELVRPLLYYKFKLGLFEEPYVDPAQIRNEQRLVRERDLALR
                     AARETMILLKNEGDLLPLDPARSSTIAVIGPNADRQMLGGYSGKPRYYRSVLEGIIEK
                     AGKNARILYSEGCKLTVGGSWNQDEIVLPDPEENRQSIAEAALVAEQADVVILVLGGN
                     EQTSREAWAKTHLGDRTSLDLPGQQNELVQAVLKTGKPVVLLLINGRPNSITAIEKEI
                     PAIVECWYLGQETGYAVADVLFGDFNPGGKLPVTIPRSVGHIPCFYNHKPSARRGYVF
                     DDISPLYPFGYGLSYTTFSISKVRLEKEVIAMGESTRVTAEVRNTGERAGSEVVQLYI
                     RDSFSSVSRPVKELRGFSKIQLAPGESKQVTLAITPDDLAFTTIDMEHKVEPGQFEVM
                     VGSSSRDEDLTRLILQVRADH"
     gene            complement(17107..>17862)
                     /locus_tag="PKM23_03045"
     CDS             complement(17107..>17862)
                     /locus_tag="PKM23_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNY90481.1"
                     /translation="EFPHAHRVIRSTAEIDDLPIPDPATDGLLPFILNRLRLAQPAIE
                     AAGHRIRFAVARGPLNIASYLMGSTEFLMAMMLEPEKAEALMKKITAFLKSWLQLQMK
                     TFPSIDGIFLLDDIIGFIGENEFRSFGLPYFKELYDLPVSVKFLHNDAACKVSAPYLP
                     EIGVNLFNMGFDITLTALKELTGSQVALLGNLPPRDVLAAGTPEVVEQATRELAASLA
                     ESSRIILSCGGGMPPGVSTANIAAFIRGAQARI"
BASE COUNT         3492 a         4738 c         5810 g         3722 t
ORIGIN      
        1 ccgcggctta caagtatgtt gatgaggctg atatattggc tggtgatggc gttgtcctgc
       61 aggacgttat attcgttgat gagcagtttg gtccccgggg cgcagtactt gcgcgccagt
      121 tcgaaggagg tgatcaccca gtcccagcca gtggcgccgt cgccgcccag ggccgcccgg
      181 tattcggccg gggcgcggat gggctcgttg acgacgtcga cataatccat tttggggtag
      241 cgttcgccga ccagtttgat ccagttttcc acggctgccc gttgggccgg ctgatcgagg
      301 gcggtgatcc aaccgggctg ctgctgtccc cagaccaggc agtggtgctt gtagagcaat
      361 cccttgctgg tcacatagtt ataaatcttg tccaggttgc tccagttgaa atagttttgg
      421 gcgggctgga cggagcccca cttgccgtca ttacccgggg tcacctgatt ccagtatttg
      481 accatatagc gggtgtactc gctgctggtg ctgcagccga ggaagcggtc cagcccctgg
      541 gtgaggggct gccccgcggc aggcgatggc agaaatccgg ccagcagcgc gaggccgaca
      601 agaaccaggg tttttacagc catgatcggc ttcgccggtc ggcgaggcag ggtgatgcgg
      661 gtgtccatat ccagaccttt cgagaataag ctgtgagtac ggttcagtgc ctgacgttgg
      721 ttcgctgcac gccggatgtg ggcatcagcg tgcaggggga taggcgccaa tatggcgcat
      781 ctcccgtgtt ccagctgcgc tgcgcacggc cgcggggggc tgggcaccgg cgcgggccgc
      841 gccaaacccg ggcaggagct ggtaatggcc cagttcgctg cttttgaaaa gggggccttc
      901 ataatcgcag tattcgaggc tggcgccggt gaattcagca tgattcgcct tcagctcctc
      961 cagactctgc agggtggcct ggcaggtggg cgttgaggcc gggctccaga gcaggagggg
     1021 ggccttggcc tggccgggag cgcgcacaaa gagattgtga tccatgcgct ccaccatcgg
     1081 ctcagcgagg cggtcgcaga gagagggggg ctgccagacg aagagcagcg gccgctcgaa
     1141 gccggtcgtg gcggtgaaga ggttgttgac gaggatgtgg ccgtcgcgct cgtcgacgcc
     1201 cggacccgtc gccggatgcc agccgaagtg gtcgccctgg gcgctgcgtt cattgcggcc
     1261 gatacaggct atgctgttga caaaggtatt gttgtagatc tgcgcatcgc agctgttgag
     1321 gatcatcagg ccatgatcgc agttgacgaa aagattgccg gcgcaaatga ccccttttga
     1381 gatctcgaaa aagaagccgt tgtcgctcgg ccagagctgg tcccggcgga tatcggtttc
     1441 gatcgagccg acgttttcga tccagttgtt gacaaagacg ccgtccacgt tgccgacatc
     1501 ataccagatg ccgttcgacc agggcatatc ggtgaccagg ttgtcccggc aggttacccg
     1561 gtagcactgg ttgaagatct tgacagccgc cggatagtag ccggtgatgc gctcgatgtt
     1621 gttgcgggtg atgatgttct tctcgagcag cacgtcgttg gagctgagaa tatagattcc
     1681 ctcggtgctg gtatcgctga tgcggcagcg gcggatggtc aggccatcgc cgcggaaata
     1741 acccgccacg cgcgagcaga aggagatggt gcagtcctcg atggtggtgc cgaccaccat
     1801 cttgccgtgg ccggattcgt ttgaaatccc ctccggatcg ctaccctcga tctccagagc
     1861 acgataggcg tactggctga aggtgaggcc gcgcagggta tagccgcggc cgtccgattt
     1921 tacgccacgg atctctttgg tcgtccttag tatggcgcag tcatgcgcgg tgatctcgat
     1981 cagatgctca gcggggtcga caccaatgta gacggttttg gtatcataat caatgaaaaa
     2041 gctgttttcg tcgacctcgc cctcataccc tgccgcctgc agcaagctgc cgtcggcgaa
     2101 gaccatgtcg ttattaaaac gatgcagggg agtctttttg ccctcgcggc tgcggcgcca
     2161 ccaatctgcc ggcttttcgg ggaagaggcg ctcccaactg gttgtccaga gcccattgcc
     2221 gaggttggtc cactggtccg caacccggct tcccttcaga atgggcagtt catcggccca
     2281 gggctgcaag gtcaccccct ggttgaggac gaggctgcca gtgcgataga ggccgccgcg
     2341 caggatgatg gcgtcgcccg agcgggcggc ggcgaccgcc tcctccaggg gcatgggctc
     2401 agccagggag gcgccgctgg cgccagcggg ggcgtcggca gcgacataga ggatcctggc
     2461 cgcgcctgcc ggcagggcat agttctgccg cacggggccg taaggcccgc ccgagggctg
     2521 ggcgagcgct gcagccggaa agtggagggt gcaccagaga agcgcgagca cggcggcagc
     2581 gcaaagcctg ccgggccgtc gggtctcatg ggcataagag tcagttttca tgatcaactc
     2641 ctcttcaggc ttatttcagc aggatgaacc ttttggattc tacggaacgg ccgctcctca
     2701 aggtgtagat atagatgcca cccgccagcc cgcggccgtc aaattcggca agatgacttc
     2761 ctgccccccg ccggccttcg aagagcaccg cggccagctg acctgtgagg gtatagacgc
     2821 gcagggagat ctcctcctcc tgggccagag tgtaggggat tcgggtgaca ggattgaagg
     2881 gattgggata gttttgcccg agcgtaaagg tggtgacgct tcctccggtt tccagagcga
     2941 ccgcgctggt tttgggaatg agagccagca gggaggcagt cagccccgtc ttcgctgtct
     3001 tgagggtatc ggccgcggag agtgagttgg catagctgcg ggtcattacc gcctgtgcct
     3061 tggccagggc ggcggttaga acagaccacg aatccacggt gtagaggctt tcctgcagcg
     3121 tccctgcctc ggcgatgagc gagtccagag cattatagga gaggccgaca tcgaccactg
     3181 cataaaaggc gggcttgcac tggtttttcg tatcccagag ggaagaggac tggttcttct
     3241 tgaaggtcca ctcgtcgttg aggccatcct tgcagacggc gatgatcttg ccgcggccgg
     3301 agaaatagct gcgctcaacg aagcatttga agagctggcc gtactggttg gcctgggtct
     3361 caagcaggcg cgcggagggc gtggcgctgc cggtggtgac gtccagttcg gtcaccgcca
     3421 tctcggagca gatcggatag aacttgtcgt acgaggcgat ccactgctcg gcggtcggcg
     3481 aagagttgga gtcatgctcc tgcatgccga tgccgtccag atagccggcc tggaagatgg
     3541 gggtgcagat gcgcacgatc ccgtccgcct tggcggggat gtgggtgttg tagtcgttgt
     3601 aatagagttt gatctggctc tcgcccagcg cgagagtgtg cttgcgggtg agttggaagg
     3661 ccttgagcag atagctgttg tcgccgaagg tctggtacca ttcgctgttg tcggtgcggt
     3721 cttcgccggc gtcggtcacg gcctcattga cgacatccat ggcgttgagc agtttcggcc
     3781 agccctcgtg gagaagtcgg atgacgctgc cgatgtaatt gtccaaccgc tcgatcattt
     3841 tctctttgga gaggcgcgcg ccgctggcgc tatagccgga gcggaagaat tcggcggggg
     3901 tttggctgtg ccagaccagg gtgtggccgc ggaaggtgaa gccctgccgt tgtgcccagt
     3961 tgagctgggc gatgaggttg ccgttgaatt tgacgatggg atgggtgtcg atcgagtcct
     4021 tcttcgcgat tgaagcggct ttgtaggcgg cggcgctggc gctgatatcg accgtatagg
     4081 ccggcttcat gttgttaccg ggtgacatgc tgttcatgtg aaatttgatc aggtggcctc
     4141 cattgggggc gaccaccgcc gtctggccgg tgacctgggg gtcactcggg aagccgacat
     4201 gggggtagga gagcaggcag ccgatgtaaa aatccccggc atagacctcc tttagggctg
     4261 gtacgtcggt ctggatggca tcggactggg ggaatgcgcc cacggcgaac agcaggagca
     4321 gagcgcagag gaggtaagga cagcggcgaa gaggattgct tttcatgaca ggccttcact
     4381 ttataaatga aaagagcatt gaaaaaacag cctgtccaga gcacctggac aggctgtttt
     4441 cgtaacacgg ttgccactcc cgggcatgcg cccgggagtg gcggaatgcg aaacagcgtg
     4501 attacttcag aagcatcagc ttcctggtgc tggtgaactc tcccgccgtc agcttgtaga
     4561 aatagatgcc ggaggagagg ttaatggcgt taaagttgac cttgtggacg ccggcgttgt
     4621 aggtgccgtt catcagctct gcaacgacct tgccgagagc gtcgtaaacc accaggctga
     4681 cctggctgtt tttcggcagc gagacttcca tggtcgtggt cgggttgaag gggttgggat
     4741 agttctggcc gagggcgtaa accgtcggaa gggcagcggt ctgttccgga gcctcaaccg
     4801 agaccaccca atccggcagc tcgatcggag cgcccttgtc caccaggact tccatcaggt
     4861 tcatcgccgt ggtggatgca ttagtgttga gagcgaagag gatgctgttg aacttgtcgg
     4921 tcaccagcgg gctgtgcatg tcggtggtct tgactgccca ggcgtaggtg ccatcggttt
     4981 taagcagttt gacccggatt tcgctggtgc cgttgcccag aggctgcacg gagatgccga
     5041 actcgtaggt gcctgcgccg ccgacggcct gatcgggccg ctgcagaacg ctgcccagca
     5101 cgtagttgtt ggcgccgctc gtgctcagcc agggattatc aaccactgct cccagggtgc
     5161 ccttggcgcc gataccctgc cagtccaccg ggccgttggc gccgctggtc ggcaggaaga
     5221 ggtatgcaaa gtcgtgcgca tcagtgcccg tccacttggt ggagtctaca ctggttgtgt
     5281 cgaccttgcc ggcgctgtcg ctgtaggcga gggcaaagcg caagcttctc accgcctcaa
     5341 aaccaccgcc gaccagttcg atcttgccgg tgatgctgag ggccttttcg ggggtcggag
     5401 cgatggcgtc gaaaccgccg cgaagcgcag accagccagt attcggagca tcgcccgagg
     5461 cagtggcatc gccatcaaac tcgccgcgga tgaatttcca gccgccgagc ctgccgccgt
     5521 agaaacccca gctgtcgaca tagtaggctt cccacggggc ttccggaacg gtgatgggct
     5581 cgcccatgtc gacctcaacg gcgtagaaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn cggcgccgcc gttgccattg gacatggtgc cggtgccggt gcggggatgg
     5761 aagccatagc cgaaatgctt cttggtgctg acccaggccg cggaatcggt gtactgatag
     5821 ttgagcaccg agctgtcctg ataggtcagg gcgtaacgga gatgggtata gttgctggcg
     5881 ccgccgccgc cgaccagttc catctggccg cggacgatga ccgccttggc cagggtggcc
     5941 gtgaccggat cgccgaaggc gccgcggatg gtggcccatc cggtgggaat ggcgccgttg
     6001 ccgagggtgg catcgccgtc aagataggtg gaatcgttct tgaccggcca ggccgtgccc
     6061 tgggtggtct tgccccactg gctgacgtag aagggctccc atggggcttc cggaacggtg
     6121 atgggctcgc ccatgtcgac ctcaacggcg tagaaattga cctgggtggc ttccgtgtcc
     6181 ttgttgaagc caaagcagat gccgttgaac ttggtggtca cctgggcggt gtcgatggtg
     6241 gtgccgccgt accagtactt gttcttctgc tcgaccatgt accagcgcac ttcgttgttg
     6301 ccgtcgggca ggggctggac cgagatggcg aaattgtaga tcccggcctc catgacggcg
     6361 tcacgcggag cctgcttggc cgccgagatc ggcagaccgt tgttgctcca ggtgctgctc
     6421 cagctgccgt ccttgatggt ccagaccacg ccggcgccgc cgttgccatt ggacatggtg
     6481 ccggtgccgg tgcggggatg gaagccgtag ccgaaatgct tcttggtgct gacccaggcc
     6541 gcggaatcgg tcttctgatt cttcaaggtg gcgctgtcct ggtaggtcag ggcgtaacgg
     6601 agatgggtat agttgctggc gccgccgccg ccgacaaatt caagttggcc atagaccacc
     6661 actgcctgtt cggtggtcgc ttccatggtt tcactgaact ggccgcggat ggtggcccaa
     6721 ccggtcggga tggcattatt gcccataccg gcatcaccat cgggagtggc cgcggtattg
     6781 aggatcggcc aggctgtacc ctggggggtt ttgccccaat ccttcacgat cgtgatatcc
     6841 accatccgga tgatggtaaa atcatcgatg tagaccgccg gggtggccgc ttcattgccg
     6901 gctttgagca ggacctgcac gccgatgcgc tgcagcggcg tcgcgacatc attggccatc
     6961 tcgcgcgtga tcgtcgtcca ggcatcgccg gtgatatccg aaccgttgat ccagatgctg
     7021 ttccacgacc agccggcgcc ggtctgccag aagagctgca cgccattgac catagcctga
     7081 tctgctgcgg acatccagat cttgaagctg acctgatcgc ccttcttaag atccttgtag
     7141 gcgtcatttt gcagattgat ctccaggccg gtagccggct tgaccagctt gatggactgg
     7201 gttccgccgg cggcctgttc ggcgctcaac gaaatggccg atttttcgcc gccgctcatc
     7261 caagagccca acgtggtctc aaaatcccac gtctggatgg ctttgggaat ttgcgcctga
     7321 agcgtgacgg cgcctaacag cagagcagcc acgactccga ggaaaacaat ggcaaacttt
     7381 ttcataagcc gattttctcc ttacgatgaa ttgcatccag cagatttttg ggggcgatcc
     7441 ggcacccctc atacactcgc aatgaggaat gagcaggttc cggataggcc cccaacctta
     7501 acttggcgct tctgaaaaag agcgaaaatt cagctttaag gcattcacct cctttggcag
     7561 ggtgtacggt cccatgagat ttaggatcga gtgtgctcct tatttggtca gaacgaattt
     7621 cttcgtgatc gtcatgttct ccgcctgcag acgatagatg tagacgccgc cggccagtct
     7681 ggcgccgtcc acattcgcag catagcggcc ggctgcctgg acgccgttga agagcgtcat
     7741 aacttcctgg ccgagcatgt tgtaaacctt caaactgacc tggccttgga cagggaccga
     7801 gtattcgatg cgggtggtcg gattgaaggg attgggataa ttctgttcaa ggctgtagct
     7861 cttcggcagg gcgctgcccg gcaccatatg gacgccgacg gtgggatcaa caccggtctc
     7921 catccaggtg gcaaggcggg cgttttcggc atcggcctgg gccttccagg cggtgtaata
     7981 gtcggtcgag ccgtcgcgta cggctggatt ccaccagttg tagagatcgc cgagcgggaa
     8041 accgcccatg ccggcctttt tgagcgtttc attggtatag gccaggtttt cgggcagggg
     8101 ccactgatgg ttaaagctgg cttccggcat gtaggcccac atcgaatcga gattggcgct
     8161 ccatttctgc cagaggaaat acttgagcgg ctcgaggttg agggggggct ggatgaagcc
     8221 aggattgatc gcctctctca actcattgac atcatgcagt tcggcgcggt tgatgaaggg
     8281 atagagcttg accccgttgg cggtggaatc gaaatagacc agagcgaggc tgtcgagcat
     8341 cgggcgcggg taggggatgg catcgaagcg tcgccccttg tattcgtcct tgctgtaggg
     8401 attgccgacc gtgttgatgg caggattggg gcgccagggg gtgccggagt aatccttgtc
     8461 ccagccgccg cgcatccagt ccaccagcca gttgtccaga tagtaggcgc tatgcgtgaa
     8521 gaggatgtgg cgatcctggt cagtgaaggg gacggaaaag gcgatcgagt ccgcctgctg
     8581 aatggtcacg gtcgcgccgc tcactccctg aaccggcacg tagcccatca tccaggcgtt
     8641 gacgaagagg cagttgttca ccttgagccg ccaccaccag ccgctctcga gggggaattg
     8701 gacaacattg tagaatgtac agtgattgaa gctgacgttg tcgctgtaat tggttccctc
     8761 ctgcatgaga acgtagccca tgttggcgaa ggtgcagttc tcaaaggaga tgttgtcggt
     8821 gtgatagccg gggacgtcga aggggaagga gaccgcacgg ccgtagtaca taaagtgggc
     8881 atcgacgcag ttgcggaagt agcagttctt gaaggtgccg ttgaagtgcc tgctgcgcac
     8941 acagatcgat ccggaggcgg tgacgacagg acagggcata tagtcgaaga tgcagccttc
     9001 gaaatagccg tattcgccgg tgtcaccgga gaccccggtg gtgtcgccat caaagacgat
     9061 cggcgtgccg gtctgcacgc cggcggcatc cgcgtagcgg acccagatgt ttttcatgat
     9121 caggtcgcca tagaccgcga tgatgaaatt tttggtgacg ctgctgctgg ccgtccagag
     9181 gatctggggc ggcgcggagg gttgggtcgt acccggcgcc ggggcgacga gttccagggt
     9241 ctgtccgatc ggaacttcga tctggccggt cagaacgtac cagtcgtagg gattcagttt
     9301 gaagaccttg ccgttgaggg agcccgaatt gacggcatcc tgaatcaccg tgttcagctc
     9361 accctcgaca cccccactgt agagaccttt gacgtaggtg gtatccttct gctgcgcgaa
     9421 cagggatacc gacaaaagca tcgtacagat cagaaacact atcaaacgtc tcataacccg
     9481 ctcctccatg ttgatgattt ttggtgaatt gtgtgagttg gttttttctg cttgttgttg
     9541 ccggggctgt gaggacagcc ccggcatgag tgatcagggc atggaacagc tctgctgcag
     9601 ggcgggaggg ctgcctcccg tgccgggcta gagtctgaag cggatgccca aattgccggt
     9661 catgccataa ttcttgacgt tggtaaagcc gtcgatcgac atctgcgccg attgattgtt
     9721 gcgattgttg atgttggtga ggtcgagata gagctccgaa ccggccaggg gcagggtctg
     9781 acgggctgag gcgtcgatgc gcaggtaatc ccgagtatag ccgtcctgtt ccgggaaggc
     9841 gccgacatag ctgaccgagt tgccctggaa catgcacgag acgcgcgcgg agaagccctt
     9901 gtattcgtag ccgacatagg cattgagaag gtcattcggt tgatagatca ggcgcccggt
     9961 gcgcgtgctg tcgaaggtgg tggtcaccgg cggccgggtg gtatagtcgg tcacctcatc
    10021 ccgcagggga taggtggctt cagattcgat tttggtataa ttgatgccga gaacgatgcc
    10081 atccaggccc cagggcatat accagagccg ggtctgaaaa tcgagctcga atcccttgac
    10141 cagggcatca taggggctgt tgatataggt atagagggtg gccccgtcgg ccggtttgat
    10201 gcccatgata ttaaaatcgc tgatggtctt gatatccttg gattttgagg tcggatggag
    10261 aatataggtg gtgtagtagg taaattcctt gatggtcttg taaaaaccgc cgatggagaa
    10321 gaggcccagc ttgttgccgt ggaaggtcag ggtcaggtca tggttgtagg cctgggccgg
    10381 tttgagatcg ggattgccgg agcggatgtt cttgcgcgga ttgtcaaagg tgaccttggg
    10441 agacatctga tgatagtccg gacgggcgag ggtatgggtg taggcgtaac ggacgtcgaa
    10501 ccacctgaag ggttcatact tggcctgaac catcggcagc cagtagtcgt tcctggggtt
    10561 cacaatcacg gtgtcgcaat tctgggcatc aggattgcgc atatcgaaca tgttgtaagc
    10621 ggtatattct gactgcgact cttcgtagcg ggcgccgccg atgaccatca gattcctgaa
    10681 tttgatctcc gacatcagat agccggcata gaaatcttca ctatactcgt acgagttggc
    10741 cagggtctgg aacatgccgc tgtaccatcc gccggtctga tcagcgccgc cagtggcctt
    10801 gccgcgccac tgcggtgagt cagccagata gcgggccatg gcggtgagca tggtgggatc
    10861 gcaggcccag taaatctggc cgaactggtt gtcgagaaag ggatccatga gatgctcgtt
    10921 gccggtgaaa ttggaggcgg caaacttggc ggtcgaagga tcgaccgcaa tgccaaaacg
    10981 attcgagaga tcattgacca gcgcggtctg gaaagccccg ctcgaacgga tgctggcgta
    11041 gggggtcttc tggtcattgg aattcgtctg catgcggtag accccgccca gcttgaaata
    11101 gcccgaaagc gaggagccaa ggcggaaggg gagcttgaag ctgggagtaa aactggtctg
    11161 gttctccttg tacagggagc tgaagaggtt gatattgtcc agatagacct gatcgtcgcc
    11221 tggataacgg aagagctttt tcaaactgtc gggaataacg ttgaccggca ctgatccggt
    11281 gatggccggc cctgtcttca actccacaaa aggagactcg ggcagattgt tcttcgaggc
    11341 ggtgttggcc gccttgagat ccacctgcag ccagttgaaa tcgtacttga actcgagcga
    11401 attgacgctg atgtcggtgt tgttttcgcc ctcgcggtag cggaaattga gcagcttttc
    11461 cgaatagttg agtacggtgt tgttgtcctg ataatcggaa ctcaggcggg tgtacatatt
    11521 gaccgccttg atggagccgg agggcagttt gtagtcgagg atgaggttgc caccgaagcg
    11581 ctgacgggtt tcaatatggc ggctgagggt ggtattggtg acccggaccg gacgatagcc
    11641 gctggcggca tcgatcttgc tgctggcggt attgtacgag gcggacatgt tgtcggcgtc
    11701 gcggtcgtac tgctcgacat tgccgagtgc gtagacaccc agcttgtcat tgaaaaaacg
    11761 tttgctgatc gagccgaccg cgcggtagtt ggcggcattg ccagtcttct gggtgtagcc
    11821 ctgctgccag agcagatcgt aatggagctc ggagggggcc tcgcgcagct ccatgttgac
    11881 cgtaccaccg atggcgttgg cgtcgagatc cggggtgagg gccttgaata cggagatcga
    11941 tttgatcatg tagggggaaa ccatggagag atcgacgctg cggtcgttga ccacgctccc
    12001 ggcggtgttg ccgagggcgc tgacgcccat ctgtgaacta ccggtggagg ccagcttgac
    12061 cccctccagt gtgaccgaat tgaactgcgg ggccagaccg cgaatgacga tcttgttggc
    12121 ttcgcccgag ctttccaggg tggagacgcc cggcagccgg ctgatagccg cggcggcgtt
    12181 aaagtcgggc agctcctgga tcttggcctc cgagaccacg ctcgagattc tcgactcggc
    12241 cagctgctgg ttgatcgcct gcacctgccc ctgcgcctgg gcggtgatga catactcctt
    12301 tcccttcaaa gcctcggctc tgagggcgaa atcatgccgc accactccat cttccttgac
    12361 tgtcaccggg atgttcaccg tggcgtagcc gatgtaggag actgtgatgg tctgctggcc
    12421 ggttggtgcg ttgggaatgg tgaaattgcc gtccaggtca gcggtggttc cgatgcgggt
    12481 tcccttgatg atgatgttgg ccgagggcag accctgtttg gtatccctgt ccgtgacctt
    12541 gcctttgatg atgccggtgc cggccaggag caggccgggc agcaggacca taaacaaaat
    12601 cgcgcagcag aacgcaagaa agcggctgga gcctgacgca ggatttacca tagcaagtgc
    12661 ttttttcata atggcgtgtc cttcttttgt ttccctggtt caagtctggc cagagcagcg
    12721 cggcggcagc agcggcacgt gcgccctcgg attgtgcgta tccacagcct cgcgggccgt
    12781 ccttaatccg gccgatcata aaaaaagcgc atcctccggc ggtatgcggc ccgacccggg
    12841 gccgcggaga ccgaaagaaa gcgctgattc gctgcaacag taatcatcgg ggtcttcccg
    12901 ctcgtaagga gctcgaattt aatgagatgg agccagcgat gattgattca aaacaacttt
    12961 gaatcaattc catattacac aaaatcatgc ccattgtcaa gaaaaaagca gtctggcgcg
    13021 gctggcttgc ctccccctgc aaccagctca attcacttaa tagctgcggc ggcgctcggc
    13081 cagttcgtcc tggatctgca ggctcatctg cttgctgatc ccgtagaagg cgagggcggc
    13141 gacgccgatc aaaaaggtaa tcgccgggta gatgctggcg gtcatgcgga tgccggcgag
    13201 ggcttgtcca tcctgggcga cattgggctg atagccatag agggagagga gccagccgca
    13261 gatggcgccg ccgaaaccga gcccagcctt gagggcgaag acaatgcccg cgaagataaa
    13321 gccagtcgag cggcgggcgg tcttccattc cgaatagtcg gctacatcac ccatcatggc
    13381 ccagagcagg ggaatggtaa agctgtaggc aagggacttg agaatattca ggacaaaggt
    13441 ccagcctacc gcttccctgg gcagcagata aaagagggca gtgaagaggg cggtgagggc
    13501 aaggccgacg agaaatactc cctttttgct gaagatgagg gtgagacgtt tggagaggat
    13561 gatgccggtc agactgatga tggtgccaac catattgaaa aggctgaagc cgatgatcga
    13621 agcgttgctc aggtcgggtt tggcgagaag gccgatggcg ttgagaaatt tatgccatcc
    13681 gctcagaacc gcctcgccgc ctgcagcacc cgccggagcc gccatgccga gcgactgcag
    13741 gaaggagaag aggtgctcct tgctgatgta atagttgaag taatagtaca tcgcactgcc
    13801 ccagagggcg agggtgatga agacgaagag ggtgagcagg aacatcgaca tccaggggcc
    13861 gttgtgaagc aggccagtga cgtcttcctt gaaggaggcc ttctggccgg gcgccggttt
    13921 gatgcgctcc ttgacgctca gaaaggtgat gatgaaaaag acaatgctga gcagggagag
    13981 gatgccgatg ctcctcgacc aaccctgctg ggcgctgccc cggcccagtg atgccaccat
    14041 gagcagcagg aaaccctgga ccaggatagt ggcggactgg gcgaagatga agcgccagga
    14101 ggagaggctc gtccgctcgt tgacatctcc cgtcattacc ccgttgaggg ctgaataggg
    14161 ggtgttgttc atggagtaga cggtcatgag caggatgtag gtaatatagg cgtagatgag
    14221 tttgccgttg ggcgaaaagt tgggggtggt gaaggtcagc cagaagagta cggcgaaggg
    14281 gatggcggtc cagagcagcc agggcctgaa cttgccccag cgcgtgttgg tccggtctgc
    14341 gatcgagccc atgatgggat caaagatggc atcccagaag cggccgacca ggagcagggt
    14401 gccggcggct gcggcggtga tgccaaaaac gtcggtgtaa aagccgacct ggaacatgac
    14461 catgatctga aatacaaagt tcgccgaggc atcggcaaag ccgtagccga tcttttcagc
    14521 cagcggcagt ctgtcgatct gttccttatt catgcttgac cttttccctc ttcaatgagt
    14581 gataaaatac ccctgataat gtgcatcagt ggtccgcccg gacctggagg atgagtctgg
    14641 tcagatcctc gtcccgcgaa gagctgccca ccatgacctc gaactggccg ggctcgacct
    14701 tgtgctccat gtcgatggtg gtgaaggcga gatcatctgg ggtgatcgcc agtgtcacct
    14761 gtttcgactc tcccggggcg agctggattt tgctaaagcc ccgcagctcc ttcaccggcc
    14821 ggctcaccga actgaacgag tcgcggatgt agagctgcac cacctcgctt cccgcccgct
    14881 caccggtgtt cctgacctcg gccgtcaccc gcgtcgattc acccatggcg atgacctcct
    14941 tttcgaggcg gaccttgctg atgctgaaag tggtatagct caggccgtag ccgaaggggt
    15001 agagggggga gatgtcatcg aaaacatagc cgcggcgggc ggagggcttg tggttgtaga
    15061 aacaggggat gtggccgacg gagcggggga ttgtgaccgg cagcttgccg ccgggattga
    15121 aatcgccaaa gagtacatcg gcgacggcgt agccggtctc ctggcccaga taccagcact
    15181 ccacgatggc ggggatctcc ttctcgatcg cggtaatgga gttgggccgg ccattgatga
    15241 gcaggaggac caccggcttg ccggtcttga gaacggcctg caccaactca ttctgctgcc
    15301 cgggaaggtc gaggctggtg cggtcgccga ggtgggtctt ggcccaggcc tcacgcgagg
    15361 tctgctcgtt gccgcccagc acgaggatga ccacatccgc ctgttcggcg acgagggcgg
    15421 cctctgcgat ggactgacgg ttctcctccg ggtcgggcag gacgatctcg tcctggttcc
    15481 aggaaccgcc cacagtgagc ttgcatccct cgctgtagag gatgcgggcg tttttgcccg
    15541 ctttctcgat aataccctcg agcaccgaac ggtaatacct gggcttgccg ctgtaaccgc
    15601 cgagcatctg gcggtcggca ttggggccga tgacggcgat ggtgctgctt ctggccggat
    15661 cgagcggcaa gagatcaccc tcattcttga gcaggatcat ggtttcgcgg gcggcgcgca
    15721 gggcgagatc gcgctcgcgc accagccgct gctcattccg gatctgcgcg ggatcaacgt
    15781 agggctcctc aaagagtccg agtttgaatt tgtaatacag aagagggcgc accagttcgt
    15841 cgatgaccga ctccttgagc cgtccagaac ggaccagttc gggcagatgg gggtagcagt
    15901 caccatccgg cagctcgatg ttgacgccgg cccgagccgc gagcagggcc gcctccagct
    15961 ggttctcagc cagcccgtaa ccgactgtgt cctccttgta aaagagctcg gtgatggcgc
    16021 aatagtccga gaccagcata ccggtaaaac ccatctcctc gcgcagcaca cgctggagca
    16081 gccagacatt ggcatgggag gggatgccgt cgatctcatt gtaggagggc atgacagcgg
    16141 ctggtttggc ctccctgacc acctggcgga aggggtagaa aaagatgtcg cgcaggaccc
    16201 gctccgagat attggccggt ccgatattcg atccggactc cggctcgcca tggccggtga
    16261 aatgcttgag ggtggcgaga aggtgagtct tgtccctgta ctcgccatca ccctgaaaac
    16321 ccttcaccgc agcgatgccc atacgggcag acacataggg atcctccccg aaggtctctt
    16381 cggcgcggcc ccagcgcggc tcacgcatga cgtcgatgac cggggtcagg gcgaggtgag
    16441 tgccgcggct gcgggcgtcc gcagcgatgc tgctgtagat ctcctggacc agttcgggat
    16501 tgaaggtgga ggcgaggcca atgggttggg ggtagctggt ggcgtctttg gcggcgagtc
    16561 catgcaggca ctcttcatga aagatcaccg gaatgccaag gcgggtctgt tcgataaaat
    16621 atttttgcag ccggttggtg acctctgcga gggctgcggg ggcgagtccg ccgaggaggt
    16681 cactcggtcg ggcgatctgc cccatgccgt ggcacaccac tgcttcatgc cctccgggtc
    16741 gagatcggtg gtgccaggct tgaagatatg ttcacgcagc cgccaaaggc agaccatctg
    16801 ggcgaccttc tcctcgaggg tcatgcggcc gaggagatca tctacccgcc gctcgacaga
    16861 aagctgcgga tttttgtact cagggtagga tgttgacggt gcgggggcgg cgggtactcc
    16921 aacgcccatc tttttacggt ccatggcaag cctctgcatc aagatgaaag gcggaagggg
    16981 aacaatttaa aaggttttaa gtaatttgaa ccgattcaaa tataaagagt actctttata
    17041 aagtcaagca aaaaaagaga taatttcaga ccgccagagg gaggaatgag tccttaaatc
    17101 ttcctttcag atgcgagcct gagctccccg tataaaggcg gcgatattcg ctgtgctcac
    17161 ccccggtggc atccccccgc cgcaggagag gatgatccgg ctgctttccg cgagggaagc
    17221 tgccagctca cgggtggcct gttcaaccac ctcaggggtc ccggctgcca gcacatcacg
    17281 cgggggcaga ttgccgagca gggccacctg gctgccggtc agctccttga gggcggtgag
    17341 ggtgatgtcg aagcccatgt tgaaaaggtt gacgccgatt tccggcaagt agggagccga
    17401 gaccttgcag gcggcatcgt tgtggagaaa cttgaccgaa accggcaggt catagagctc
    17461 tttgaaatag ggcagtccga agcttctgaa ttcattctcg ccgatgaaac cgatgatgtc
    17521 atcaaggaga aaaatgccat cgatagaggg gaaggttttc atctgcagct gcagccagga
    17581 ctttaaaaat gcggtgatct ttttcatcag ggcctcagcc ttttcgggct cgagcatcat
    17641 ggccatgagg aactcggtgg agcccatcag gtagctggcg atgttgagcg ggccgcgtgc
    17701 gacagcgaag cggatgcggt ggccggccgc ctcgatggcg ggctgagcga gcctgaggcg
    17761 gttgaggatg aaggggagta ggccgtcggt ggccggatcg gggatgggca ggtcgtcgat
    17821 ctctgcggtc gagcggatca cccgatgggc gtggggaaac tc
//
LOCUS       DAOPWU010000029        17511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3295_length_17511_cov_4.561755, whole genome shotgun sequence.
ACCESSION   DAOPWU010000029 DAOPWU010000000
VERSION     DAOPWU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17511
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3295_length_17511_cov_4.561755"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKM23_03050"
     CDS             <1..231
                     /locus_tag="PKM23_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90482.1"
                     /translation="DPAHGEALSLEAQCRNSVTRLVDEHFNRGLNFYTEEKYRAAIAE
                     WDRALFINPDHRGSIEYKRRAQERLDALNQLP"
     gene            209..625
                     /locus_tag="PKM23_03055"
     CDS             209..625
                     /locus_tag="PKM23_03055"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNY90483.1"
                     /translation="MPLISSPERAAPAVRRPLLTQADKWLLGLGLIIGLLLSALLLPG
                     RERGREVLIEARGRLFVRAYLDTPARYEVPGPLGTTVVEVREGAVRVISSPCPEKHCV
                     RSGAVRRQGGLIVCVPNRVIVRIPGHIGGSLDMITE"
     gene            630..1172
                     /locus_tag="PKM23_03060"
     CDS             630..1172
                     /locus_tag="PKM23_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNY90484.1"
                     /translation="MSRPLTTKLTRLALLATAGLALFLFESLIPRPLPWIKPGLSQIA
                     TLIALYVYGWRESLAVVVLRVGLAALLTGSFAGPGFWLALPAGVTAVLVMAAARSAGG
                     RSLSIAGVSMIGALTHNLTQLALVRLFYVRSSEIWTLLPLVWLPALFTGLAVGLAARL
                     LIDFSRRMSLDWGTNPDGVE"
     gene            1292..3733
                     /locus_tag="PKM23_03065"
     CDS             1292..3733
                     /locus_tag="PKM23_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY90485.1"
                     /translation="MRKFHALSLLGLTLILFSGCAYYNTFYNAEQFFNDAAKERKKRE
                     RTQVVELSPEEQQEARRRGELTTSSTANKPTASEMQNYQKAIEKASSVLEYYPKSRWV
                     DDALIMVGECFFYRGEYSKAKRKFEEIIQLYPGSEFIPQAEILLGKTLLAVGEFDAAE
                     KKFRDITLNTRFPKAVRQSAEYELGSLYFEKGNYEAAAEEYTRTAKESDDRLIRAMSF
                     YRLGECRIQLKQYEEAVPSFRRAVQASPNEDFKSQATYKLGESQGLLKDYEGAIRTFS
                     LLLAKELEVKRIPMIKLQLANNQRAKGDEEAAVKWYRNIIEEHKGTEASARSYYGLAE
                     IEEQSYGNYLKAKENYDLVRGEQSASLLATRAKERSDAIKSMLDLKKSINEILGIATA
                     DDSLAADGNGKEKKEELDDAPIDLGADGMWMNYTGRDRRPPRSHTPDDAAAAASMSAA
                     PGADSLQTTAALSDSAQQLLLKEEAEKKKSVTLAEKRLELAELLMFSFDRPDSSVRLF
                     LQVVESKPDSAMTARALYSIGYIIYAIKKDTLQADSLFRSLVYLYPGSPHAEGARRIL
                     GWPLLSDKVDTAGIVYREAEKAWWDEKDLKRALKLYDAIADDYPSSPYAIKAQFSKGW
                     LYEHEMSAYDKAIEAYKTIVEKYPESAYGKNLKNKLTRHEQAIQAIEARKKAIADSIK
                     QAAEKAKAASDSLRRSLAPDSTAQAAGIIDSTGSAGVAADSAQVDSTASGQSPAGSAP
                     LPGANAEGQPGGTRPQVLNPDDDEPPSPAPHKPSLEEEVEIEKAVEDKPAPPGAAIPP
                     DAKTPQNRKRPVGPE"
     gene            3753..4562
                     /locus_tag="PKM23_03070"
     CDS             3753..4562
                     /locus_tag="PKM23_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNY90486.1"
                     /translation="MSKSVLQLPVIERAEAARQVVRLTLQAPEWARAARAGHFINLLV
                     PGRRDLLWRRPFSVHRADRSRGTIDLLVARMGRGSESLAECAPGDHLDAIGLLGNCFP
                     FDPGLHEIIMVAGGIGIAPFDLLLQDVQGFSCKKSLFYGARSAGDLCRSTLWAESGAE
                     VHLTTEDGSTGSRGLVLDELRRYLGSSANHAGRVIYGCGPTPMLAALREMALALGIKG
                     YVTVENRMACGFGACVGCAVELAEARRDGQKYLLACKDGPVFPIEEILFHD"
     gene            4555..5466
                     /locus_tag="PKM23_03075"
     CDS             4555..5466
                     /locus_tag="PKM23_03075"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNY90487.1"
                     /translation="MTDLRVHIGSLVLDNPVLTASGTFGYGSEFADFFDLDRLGGIVT
                     KTVTLEPRPGNPPPRICETPAGMLNSIGLPNVGVEQFKLEKWPFLSSLHTAVIVNVAG
                     RSSQEFADVVAALEAVKGIAAYELNYSCPNVKEGGLSFSSDARVAAEVTARVRAVTRR
                     PLITKLTPNVTSIGEIGRACADAGADALSAINTLVGMAVDIRTRKPRLSTVTGGLSGP
                     AIRPVALARVYELVRRVSIPVIGIGGIATAEDALEFIIAGAAAVQIGTAGFVHPDAAL
                     RVVEGLEDFCRREGLANLAQLRGSLQL"
     gene            5479..5949
                     /locus_tag="PKM23_03080"
     CDS             5479..5949
                     /locus_tag="PKM23_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNY90488.1"
                     /translation="MKRRSLLFCSLFTLIVSCYPNTRWWNDGADYGPEEEGQPDRAGE
                     TVSSAETSPARWREPESGIASFMADELHGRMTASGELYDMRKLTAAHPSLPFGTLVEV
                     RNLANGRSVEVRINDRGPYVAGRIIDLSFQAAVDLELTERGSGKVEVRVIRMPR"
     gene            5995..6831
                     /locus_tag="PKM23_03085"
     CDS             5995..6831
                     /locus_tag="PKM23_03085"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNY90489.1"
                     /translation="MNLKSFGIRALVALIFGPLIVLAALQGGLWWLAFVTLVVVLSVW
                     EYYGLARAKGSRAHYAPGLICALALVLSLYWFDVRLILPIVLAFFVWLQFSALYRGSG
                     SPTLEVPVTAFAALYYPLAFGSFILIRELPGAHFGLDSSPAGGWILVLIFSIWICDTA
                     AYVGGSYLGRHKLMPRISPNKSIEGTVLGFIFALLTAWLCHGWFVKGLLLQDALVIGA
                     IGGSLGQYGDLFESMFKRDAGMKDSSRLIPGHGGILDRFDSLTITAPVVYLYLYLRFF
                     AL"
     gene            complement(6907..7725)
                     /locus_tag="PKM23_03090"
     CDS             complement(6907..7725)
                     /locus_tag="PKM23_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNY90490.1"
                     /translation="MKIYITADMEGVNGVVLREHVDPASREYEMARGWMVAEVNAAIE
                     GAVAGGASEVIVNDSHNNMANLPADRLHPAARLVSGPGKPFSMMQEIDSSFAGAFFLG
                     YHAAFGTPLAVHDHTWAYSYIEDIRINSIHIGEFGLNAGLAGHYRVPPLLVTGDRAVV
                     NEARALVPGVAAVAVKEGRGRLCALCEPFEQSLAAIREQAAASVHNLPSCKPLIFAEP
                     LTLEVDFPKAEMAEAASLLPGSERTAARTIACHPGDYPQLYRCFLALFRLAKTA"
     gene            complement(7793..8749)
                     /locus_tag="PKM23_03095"
     CDS             complement(7793..8749)
                     /locus_tag="PKM23_03095"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNY90491.1"
                     /translation="MTKLEKAAQAVAGRALDIHKDESVLIIADEPFLDLARLLFTAAE
                     KRSRHSHLLQVSQSYISKHALCPAVERLMQEMNVILALTSFSISHTPGRREACRRGAR
                     FITMPAITNETFARIADMDFTRIGRLSRKLADILTIAKEIEISAPNGTRLRVGAGSAK
                     GYADTGEVTRPGAFSNMPAGEACMAPESVGTEGELVVDSGMGVQRDDRDPLHLAIREG
                     RCVRISGGDPALRLRRRLAVHGPEARVVAEFGIGTNDAARPSGYALEDEKVLGNIHLA
                     LGNNLSFGGTNDVPIHLDAVVYKASVVIDGRRIIDGGRMVLD"
     gene            complement(8746..9162)
                     /locus_tag="PKM23_03100"
     CDS             complement(8746..9162)
                     /locus_tag="PKM23_03100"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNY90492.1"
                     /translation="MAEAKAGSRQTPAENLLDYIPHRNLPWDAAADGLVTLRVARFSG
                     RLLQKYLMPHLKHPHIAVHLDAFGSWVWQHMDGRLTVREMAAGLEQEFGDKIKPVHQR
                     LGKFINMLVERSYVTLVKPAGQRTGPSGAAGEQPRP"
     gene            9450..10247
                     /locus_tag="PKM23_03105"
     CDS             9450..10247
                     /locus_tag="PKM23_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNY90493.1"
                     /translation="MAFLDDITLGQYYPADSFLHRLDPRSKLFAAFSLMTGLLLTYNI
                     YILAGMALLFLFAIRIARIPYALVLKNLRPFLWLFALTWIIHLVWSSGRVLWVVPGTS
                     ITLTREGALLGGIYAVRLALLILYAALLTLCTSPIELTDALERVSRPLKRLGVPTHEL
                     SLMLTLSLRFIPTLLEEAQRIRNAQLSRGARFTGSLRHRVRGLLPLMVPLFVSAFRRA
                     DELALAMDARCYTGGDGRTVYAQLRLTGADWGVITGSLVILAFCMGL"
     gene            10253..11008
                     /gene="truA"
                     /locus_tag="PKM23_03110"
     CDS             10253..11008
                     /gene="truA"
                     /locus_tag="PKM23_03110"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNY90494.1"
                     /translation="MRNIKLTLEYDGSGFCGWQLQPGQRSVQGEIESALGRLTGQRIG
                     IIAAGRTDSGVHALGQVINFRTASALPLEVFMRGTNALLPGDVRILAAEEVPEAFHAR
                     YDAISRLYRYQIATRPLAIGRLYTWHLPLRLDLAAMQEAAAALLGDHDFVSFCQAGSG
                     LAHHRCTVLRAEWQASGERLSFEIHANRFVHNMVRIIVGTSVEIGRGALAPSAMAQII
                     AASDRRAAGRTAPPHGLFLVQVCYGEKQPPLQE"
     gene            11011..11661
                     /gene="fsa"
                     /locus_tag="PKM23_03115"
     CDS             11011..11661
                     /gene="fsa"
                     /locus_tag="PKM23_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010870474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNY90495.1"
                     /translation="MKFFIDTANLEEIKRAKALGVADGVTTNPTLIAREPGKPDEIYR
                     AICALIDGPVCAEVVSLDTEGILREGRELAGIAKNIVIKIPVTKEGLMAVKVLESEGI
                     RTLVTLIFSPLQALLAAKAGASFICPFVGRLDDTGQVGMELVEQILSIYANYEFQTEI
                     IVASIRNPLHVLDAATMGAHIATIPLKVIEQLVKHPLTDAGIQLFLKDWESVKARS"
     gene            11715..12932
                     /locus_tag="PKM23_03120"
     CDS             11715..12932
                     /locus_tag="PKM23_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNY90496.1"
                     /translation="MTVRPRSSGPGGGRLRIALLFVTAILSLTSAAAAQRQPWHEAER
                     RGAAVNREADIQDVISNSRHNAITRAVAEVSPAVVGINVVQLQRFYQRTPFDDDPFFR
                     QFFRDHPVEKRVKGLGSGFIISREGYVLTNQHVVADATEIIVTRTGGKQYIARKVGED
                     YVSDVAILKIEGNDFPYVQFGDSDDVVIGEWAIALGDPFGLFDIGSKATVTVGVISAT
                     DRDFGRQDDDRIMEDMIQTDAAINAGNSGGPLVNCYGEVIGINTWILSGNGTNIGLGF
                     ALPINRVKRILDDLINYGAVERRWYTGLRYEAMSLMTARHLGLRSVHGVIVSEVEAAS
                     PAARGGFEIGDVIMGINGREVESFEDVKRIIDGLDLKRGDNLVFRVFRGRRYLNLTIK
                     LEYRAGGVKGEGR"
     gene            12929..14227
                     /gene="purB"
                     /locus_tag="PKM23_03125"
     CDS             12929..14227
                     /gene="purB"
                     /locus_tag="PKM23_03125"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNY90497.1"
                     /translation="MIKRYARPEMAAIWSDEHKYETWLRVEIAVCEAQAELGAIPAAA
                     VEAIKARAGFSIARIEQIEAEVRHDVIAFLTALSERVGDEARHIHLGMTSSDLLDTAL
                     ALNIQEAGALLLEGMREFRAILARQALKHRDTVCIGRSHGIHAEPSSFGLKFALWYDE
                     MGRNLERLESALEAAGVGMISGAIGTFAYIDPRVEVSACARLGLRPAPVTTQIIQRDI
                     HAQFQSVLALCGATLEKIAVEIRHLQRTEVLEAEEPFSAGQKGSSAMPHKRNPIGSEN
                     IAGLARLLRTNALAALENIALWHERDISHSSVERIIFPDSCILLDYMLHRLARIVEGL
                     VVYPERMIRNLRLTGGLIFSQPVLLALTGKGMSREYAYRIVQEDAMRCYHEDGDFREI
                     LLGDARVLELLTAAEVESCFNEQVGLKHVDEIFRRVGISE"
     gene            14273..14968
                     /locus_tag="PKM23_03130"
     CDS             14273..14968
                     /locus_tag="PKM23_03130"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF006868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNY90498.1"
                     /translation="MKKKKIYEGKTKKVYEGEHETELILEFKDDAVALSGSKPTHIRG
                     KGMMNKQISVYLFRLLESYHIGTHLVRELSDKEILVKRLNMIPVVVMVHNVVAGKLVK
                     SYGMKEGKELECPIIEYYLKDEERDDPMINEDHVVSFGHATSLEIKEMHRLASKSNAV
                     LKAFLRRREFQLVDLRLEFGRHQNRLLLADEISLNTVRTIDLRYPELGILDGDNPRVA
                     ENLAQVKSRIFSA"
     gene            15096..15875
                     /gene="pssA"
                     /locus_tag="PKM23_03135"
     CDS             15096..15875
                     /gene="pssA"
                     /locus_tag="PKM23_03135"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNY90499.1"
                     /translation="MNIKKKRTPRQRTYINVPNLFTALNIFCGFLSVLQTIEGNYTTA
                     AWLIFFAAVFDALDGRIARATGQSNEFGLQMDSLGDVISSGLAPAVLVYTIQLKSLHP
                     PVGLILAFFPVLFAAFRLARFNVFTLSEGKKSDYLGLPAPMAGVTLASCVILYQSVQW
                     DLLLRMLIGLVPVVSLLMASTIRYEGMPRFSIRERGFNRLKLILFFVITALFLVYPDY
                     VLFPFMIFYILAGICRSVFSLMRNEDDGEITLIPKEPDLKS"
     gene            15898..16188
                     /locus_tag="PKM23_03140"
     CDS             15898..16188
                     /locus_tag="PKM23_03140"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HNY90500.1"
                     /translation="MLSKDRYRIKRKFSIGMILIAVVLGALIGSTLGEAIGVILPEGV
                     VKDFLIKSASIGFSPVAVNLVILSFTIGFTFHLNIIGVIGIIIAAYILRWFA"
     gene            16281..16433
                     /locus_tag="PKM23_03145"
     CDS             16281..16433
                     /locus_tag="PKM23_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNY90501.1"
                     /translation="MFVIVLVALILFGSKQLPELARGLGKAMRELKKATDDVKRELDL
                     TDELRK"
     gene            16464..16664
                     /locus_tag="PKM23_03150"
     CDS             16464..16664
                     /locus_tag="PKM23_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNY90502.1"
                     /translation="MGVGPTEWVIIILILVLLFGAKKIPELARGMGQGLREFKKAANE
                     VQDTSSQSASRGEVGPKENKQE"
     gene            16689..>17511
                     /locus_tag="PKM23_03155"
     CDS             16689..>17511
                     /locus_tag="PKM23_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNY90503.1"
                     /translation="MLLHTGSVDFQSIKPLLAAGSISCTSLAETYLQHIDKGAHLNAF
                     ISVFAEKALEQAAAVDRKRTRGTAGRLAGLFLGVKDNIVWSSGPTTCASRMLAGYQSP
                     FSATVLKRLAEEDAIFLGKTNMDEFAMGSSTENSAYGAVRLPADPGRVPGGSSGGSAA
                     AVAAGLCTAALGSDTGGSIRQPASFCGVVGLKPTWGRVSRYGLVAFASSLDQIGPMGR
                     SVADCAELLQVMAGYDPADATSSMAPVPDYAASLTRDLKGLRIGLPREFFPQAGME"
BASE COUNT         3212 a         5426 c         5411 g         3462 t
ORIGIN      
        1 gatccggctc acggggaggc cctctccctt gaagcgcagt gccgcaacag cgtcacccgg
       61 ctggtcgacg aacacttcaa tcgcggtctc aatttttata ctgaagaaaa gtaccgcgcg
      121 gcgatcgcgg aatgggatcg cgccctgttc atcaatccgg atcatcgcgg ttccatcgaa
      181 tacaagcgcc gcgcccagga gcggctggat gcccttaatc agctcccctg aacgggcggc
      241 tcccgccgtg cggcgtcccc tgctgacgca ggccgacaaa tggctccttg gtttggggct
      301 catcatcggc ctgctgcttt cggcgctgct gctgccgggc cgggagaggg ggcgtgaggt
      361 cctgatcgag gccaggggca ggctctttgt gcgcgcctat ctcgacaccc ccgctcgcta
      421 cgaggtgccc ggcccgctcg ggactaccgt agtcgaggtc agggaagggg cggtccgggt
      481 gatctcctcg ccctgtcccg agaagcactg cgtccgctct ggtgctgtcc gtcgacaggg
      541 ggggctgatc gtctgtgtcc ccaaccgggt gatcgtccgc atccccggcc atatcggcgg
      601 ttcgctcgac atgatcacgg agtgatggga tgagccggcc gctgaccacc aaactcaccc
      661 gtctggccct gctggcgacc gccggcctgg ccctcttcct cttcgagtct ctgatcccgc
      721 gtcccctgcc ctggatcaag cccggtctct cccagattgc caccctgatc gccctctacg
      781 tctacggctg gcgggagtcc cttgccgtgg tcgtgctgcg ggtgggactg gccgcactcc
      841 tgaccggctc ctttgccggt ccgggcttct ggctggccct gccggcgggc gtgaccgcgg
      901 tcctggtgat ggccgctgcc cggagtgcgg gcgggcggag tctgagcatc gccggggtca
      961 gcatgatcgg ggcgctcacc cacaatctca cccaactcgc ccttgtccgt ctcttctacg
     1021 ttcgcagcag cgaaatctgg acccttctcc ccctggtctg gctgccggcc ctcttcacgg
     1081 ggctggccgt cggtctggcc gcccgcctgt tgatcgattt cagccgccgc atgagtctgg
     1141 attggggaac caatccagac ggcgtggagt aaacaggaag gcggcgcagg ggtggtcacc
     1201 acccctgcgg cggctcgatt tttcagttgt tatttatgcc agcaaagtgt aatttaggtt
     1261 ttaaacagag cggcagaatc gagagcatcc catgcgcaaa tttcacgccc tatccctcct
     1321 tggcttgacg ctgattctct tcagcggttg cgcctattat aatactttct ataatgccga
     1381 gcagtttttt aacgacgccg ccaaggagcg caagaagcgc gagcgcacac aggtcgtgga
     1441 actctcgccc gaggaacagc aggaggcgag gcggaggggg gagttgacca ccagttccac
     1501 tgcgaacaaa ccgaccgcga gcgagatgca gaattaccag aaggcgatcg aaaaggcctc
     1561 cagcgtcctc gagtattatc cgaaaagccg ctgggtggac gacgccctga tcatggtcgg
     1621 ggagtgcttc ttttaccgtg gtgagtattc caaggccaaa cgcaagttcg aagagatcat
     1681 ccaactctat cccggcagtg agttcatccc ccaggccgag atcctgctcg gcaagaccct
     1741 gctcgcggtg ggtgaattcg acgcggccga aaaaaagttc cgcgacatca ccctcaacac
     1801 ccgcttcccc aaggctgtcc gccagtcggc ggagtatgag ctcggctctc tctattttga
     1861 aaagggcaac tatgaggccg ccgccgagga gtatacgcgg acggccaaag agtcggatga
     1921 ccggctcatc cgggcgatgt ccttttaccg gctgggggag tgccggatcc agctcaagca
     1981 gtatgaggag gcggtaccct ctttccgccg cgccgtccag gccagcccaa acgaggattt
     2041 taaatcccag gcgacctaca agctgggcga atcccagggt ctgctcaagg attacgaggg
     2101 ggctatccgc accttctcgc tcctgctcgc caaggagctg gaggtcaagc gcatcccgat
     2161 gatcaaactg cagctggcca acaaccagcg cgccaagggg gacgaagagg ccgccgtcaa
     2221 atggtaccgc aacatcatcg aggagcataa ggggacggag gcctcggcgc gcagctatta
     2281 cggcctggcc gagatcgagg aacagagtta cgggaactat ctcaaggcca aggaaaacta
     2341 tgatctggtg cgcggcgagc aatccgcctc gctgctggcg acgcgggcca aggagcgttc
     2401 cgatgcgatc aagtcgatgc tcgatctgaa aaagtcgatc aacgaaattc tcggcatcgc
     2461 cacggccgat gactcgctgg cggccgacgg caacggcaag gagaagaagg aggagctgga
     2521 cgacgccccc atcgatctcg gcgctgacgg catgtggatg aattacaccg ggcgcgaccg
     2581 gcgcccgccc cgcagccaca cccctgatga tgcggctgca gcagccagca tgtcggctgc
     2641 ccctggcgca gattccctgc agaccaccgc tgccctctcc gacagcgccc aacagctcct
     2701 gctcaaggag gaggccgaaa agaaaaagag tgtcaccctg gcggagaagc gtctcgaatt
     2761 ggccgaactg ctgatgttca gcttcgaccg cccggactcc tcagtgcggc ttttcctcca
     2821 ggtcgttgag agcaaaccgg acagcgccat gaccgcccgg gccctctatt caatcggcta
     2881 tatcatctat gcaatcaaga aagacaccct ccaggccgac tcccttttcc gcagcctggt
     2941 ctatctctat cccggttcgc cccatgccga gggggcgcgg cgcattctcg gctggccttt
     3001 gctcagcgat aaggtggaca ctgcgggcat cgtctaccgg gaggccgaga aggcctggtg
     3061 ggatgaaaag gatctgaaga gggcgctcaa gctctacgat gcgatcgccg atgattatcc
     3121 ctcttcgccc tacgccatca aagctcagtt cagcaagggc tggctctatg agcacgagat
     3181 gagcgcctac gacaaggcga tcgaagccta caagaccatc gtggaaaagt accccgagtc
     3241 ggcctacggc aagaatctca agaacaagct gacccgccat gagcaggcca tccaggccat
     3301 cgaggcacgt aaaaaggcga tagctgattc gatcaagcag gcagccgaga aggcgaaagc
     3361 ggcgagcgat tccctcaggc ggagcctggc cccggacagc accgcgcagg cggccggcat
     3421 tattgattcg accggcagcg cgggtgtggc ggccgattca gcccaggtgg attcgacggc
     3481 gtcgggacaa tccccggcgg gttctgctcc tcttcccggt gcgaacgcgg aggggcagcc
     3541 gggtggaacc cggccgcagg tgctcaatcc tgatgatgat gagccgccct ccccggcgcc
     3601 gcataaacca tccctggagg aagaggtcga gatcgaaaag gcggtggagg acaaacccgc
     3661 tccgcccgga gcagccatcc cgcctgatgc caaaacacca cagaacagga agaggccggt
     3721 cggacccgaa taaacggaga aggagagccc gcgtgtcaaa atccgtgctg cagctgccgg
     3781 tcatcgagag ggcggaggct gcgcgccagg tggtgcgttt gaccctgcag gccccggagt
     3841 gggcccgtgc agcacgtgcg ggtcacttta tcaacctgct cgtgcccggg cgtcgggacc
     3901 tgctctggcg gcgtcccttc agcgtccatc gcgccgaccg ctcccgcggc accatcgatc
     3961 tgctcgtcgc ccgcatgggg cgcggcagcg agtccctcgc cgagtgcgcg cccggagatc
     4021 atttggatgc catcggcctg ctcggcaact gttttccctt cgatcccggc ctgcacgaga
     4081 tcatcatggt cgccggcggc atcggcatcg cacccttcga tctcctgctt caggatgtgc
     4141 agggcttttc ctgcaaaaag agcctttttt acggcgcgcg cagcgccggc gacctctgcc
     4201 gcagcaccct ctgggcggag agcggcgccg aggtccacct taccaccgag gacggcagca
     4261 caggaagccg cggcctggtg ctggatgagc tgcgccgcta tctggggagc agcgcaaatc
     4321 atgcaggccg ggtgatttat ggctgtggac ccaccccgat gctcgccgcc ctgcgggaga
     4381 tggctctggc cttaggcatc aagggatacg tcacggttga aaaccggatg gcctgcggct
     4441 tcggggcgtg cgtcggctgc gcagtcgaac tggcggaggc acgccgggac ggccagaaat
     4501 acctgctcgc ctgcaaggac ggtccggtct ttcccatcga ggagatcctt tttcatgact
     4561 gatttgcggg tgcacatcgg atccctcgtg ctggacaatc ccgtcctcac tgcgagtggg
     4621 actttcggtt acggcagcga attcgctgat tttttcgatc tcgaccgcct gggggggatt
     4681 gtcaccaaga cggtcaccct ggagccgcgg ccgggcaatc cgccgccgcg catctgcgaa
     4741 acccccgccg ggatgctcaa ctccattggt ttgcctaatg tcggggttga gcagttcaag
     4801 ctcgaaaaat ggccctttct gtcgtcgctg cacacggccg tgatcgtcaa cgtcgccggc
     4861 cgcagcagcc aggagttcgc cgatgtggtg gcggccctcg aagcggtgaa gggcatcgcc
     4921 gcttacgaac tcaactacag ctgtcccaat gtcaaggagg gagggctctc cttcagcagc
     4981 gatgcgcggg ttgcggcgga ggtgaccgcc agggtgcgcg cggtgacgcg tcgccccctg
     5041 atcaccaagt tgacccccaa tgtcacctca atcggcgaaa tcggccgcgc ctgcgccgat
     5101 gcgggcgccg atgccctctc ggcgatcaac accctggtgg ggatggcggt ggacatccgc
     5161 acccgcaaac cccggctctc caccgtcacc ggcgggctct ccgggccggc tatccggccg
     5221 gtcgctctgg ccagggtcta tgagctggtc aggagggtct ccatcccggt gatcgggatc
     5281 ggggggatcg ctacagcgga ggatgccctc gagttcatca tcgccggggc cgccgccgtc
     5341 cagatcggca cggccggctt tgtccatccc gacgccgccc tgcgggtggt cgaggggctt
     5401 gaggattttt gccgccggga ggggcttgcg aacctcgccc agctgcgcgg ctccctgcag
     5461 ctttgaccgg agggcaccat gaaacgtcgc tccctgctct tttgctcgct cttcaccctg
     5521 attgtctcct gctatcccaa cacgcgctgg tggaatgatg gagcggatta tggtccggaa
     5581 gaggagggtc agccggacag ggcgggggag acggtttcgt ctgcagaaac ctcgccggcg
     5641 cgctggcgtg agcccgagtc gggcatcgcc tctttcatgg ccgacgagct tcacggccgc
     5701 atgacggcca gcggagagct ctacgacatg cgcaagttga ccgccgccca tcccagcctg
     5761 cccttcggca ccctggtgga ggtcagaaac ctcgccaacg gccgcagcgt cgaggtgcgc
     5821 atcaacgacc gggggcccta tgtggctggg cgcatcatcg atctttcctt tcaggccgcc
     5881 gtggatctgg aattgacgga gcggggcagc ggcaaggtgg aggtgagagt gatccggatg
     5941 ccgcgctgac ctcacggttg attcgaattg taactattat gccatggagg tttcttgaac
     6001 ttaaaatctt tcgggatccg ggcccttgtc gccctcatct tcggccccct catcgtcctc
     6061 gccgccctcc agggcgggtt gtggtggctg gccttcgtca ccctcgttgt ggtgctctcg
     6121 gtatgggagt attacgggct cgcccgcgcc aaggggagcc gcgcccatta cgccccgggc
     6181 ctgatctgcg ccctcgccct ggtgctttcc ctctactggt tcgatgtgcg cctcatcctg
     6241 ccgatcgtgc tggcattctt cgtctggctg cagttctcag ccctctatcg aggcagcggt
     6301 tcccctaccc tcgaggtgcc ggtgacagca ttcgccgccc tctactatcc cctcgccttc
     6361 ggcagcttca tcctcatccg cgaactcccg ggcgcccatt tcggcctcga cagttcgccc
     6421 gctgggggtt ggatcctcgt cctcatcttc agcatctgga tctgcgacac cgccgcctac
     6481 gtcggcggca gctatctcgg ccgccacaaa ttgatgcccc gcatcagccc gaacaagtcg
     6541 atcgagggca cggtgctggg ttttatcttc gccctgctca ccgcctggct ctgccatggc
     6601 tggttcgtca aggggctgct cctgcaggat gctctcgtca tcggtgccat cggcggcagc
     6661 ctcggccagt acggcgatct cttcgagtcg atgttcaaac gggatgcggg gatgaaggac
     6721 tcctcccgtc tcatccctgg gcacggcggc atcctcgacc gtttcgacag cctgaccatc
     6781 acggcgccgg tggtctacct ctatctttat ctgcgctttt tcgccctcta gggggctgcc
     6841 ccggatatga taaagccagg ctgcgctgat gcggcctggc tttttttatt gcggatctga
     6901 ggaggatcag gccgttttcg ccagtctgaa cagggcgagg aagcagcgat agagctgggg
     6961 ataatcgccg ggatggcagg cgatggtgcg cgccgcggtg cgctcgctgc cgggcaggag
     7021 gctggccgcc tcggccatct cggccttggg gaaatcgacc tccagggtca agggttcggc
     7081 gaagatgagg ggcttgcagc tggggaggtt gtgtacggaa gccgcggcct gttcgcggat
     7141 ggcggcgagg ctttgctcga agggctcgca aagggcgcag aggcggccgc gcccctcctt
     7201 gacggcgacg gcggccacgc cgggcacgag ggcgcgcgcc tcgttgacca cggcgcggtc
     7261 gccggtcacg agcaggggcg gaacccggta atggcccgcc agaccggcgt tgaggccgaa
     7321 ttccccgatg tggatggagt tgatgcggat atcctcgatg taggagtagg cccaggtgtg
     7381 gtcatgcacg gccaggggtg tgccgaaggc ggcgtggtag ccgaggaaga aagccccggc
     7441 gaaggagctg tcgatttcct gcatcatcga aaagggcttg ccgggtccgg aaaccaggcg
     7501 ggcggcgggg tggagcctgt cggcgggcag attggccatg ttattgtggg agtcgttgac
     7561 gatgacctca gaggcccctc cggcgacggc tccctcgatc gcggcattga cctcggcgac
     7621 catccagccc cgggccatct catactcacg ggaggccggg tcgacgtgtt cgcggagcac
     7681 gacgccattg accccctcca tgtccgctgt gatatagatc ttcatgattt ctccgcagtt
     7741 ggtctaaaga gggcggtgcg gaagcaacgc cgcgcctctc ctcatctccg cttcaatcca
     7801 gaaccatccg gccgccgtcg atgatcctgc ggccgtctat gaccacactc gccttgtaga
     7861 cgacggcgtc gagatggatg ggtacgtcgt tggtgccgcc gaaggagaga ttgttgccga
     7921 gggcgaggtg gatgttgccg agcacctttt catcctcgag ggcgtagccg ctggggcggg
     7981 ctgcgtcatt ggtgccgatg ccgaattcgg cgaccacacg cgcctccggt ccgtggacgg
     8041 cgaggcggcg gcgcaggcgc agggcgggat cccccccgct gatgcgcacg cagcggccct
     8101 cacggatggc gaggtggagg ggatcgcggt catcgcgctg cacccccatg ccgctgtcga
     8161 cgaccagttc accctcagtg ccgacggatt ccggggccat gcaggcctcg ccggcaggca
     8221 tattcgaaaa agctcccggg cgggtgactt caccggtgtc tgcatagccc ttcgcggagc
     8281 ccgctccgac gcgcaggcgg gtgccattcg gggcgctgat ttcgatctcc ttggcgatgg
     8341 tgagaatgtc cgcgagcttg cggctgaggc ggccgatgcg ggtgaaatcc atgtcggcaa
     8401 tgcgggcgaa ggtttcgttg gtgatggcgg gcatggtgat gaaacgggcg ccgcgacggc
     8461 aggcctcgcg ccggcctggg gtgtgggaga tggaaaatga ggtcagggcc aggatgacat
     8521 tcatctcctg cataagccgt tccaccgccg ggcaaagggc atgtttactg atgtaggact
     8581 gcgagacctg gagcagatgg gagtggcggc tccgtttctc cgccgccgta aagagcagac
     8641 gggcaagatc gagaaagggc tcgtcggcga tgatcaggac cgattcatcc ttatgaatat
     8701 cgagggcgcg gccggcgacg gcctgggctg ctttttccag cttggtcatg ggcgcggctg
     8761 ctccccggct gcgccggagg gtccggtccg ctgccccgcc ggcttgacca gggtgacata
     8821 ggagcgttca accagcatgt tgatgaattt tccgagccgc tgatggaccg gcttgatctt
     8881 atcgccgaat tcctgctcga ggccggcggc catctcccgg acggtgagac ggccgtccat
     8941 gtgctgccag acccaggagc cgaaagcatc gagatgcacg gcgatgtggg ggtgtttgag
     9001 atggggcatc agatatttct gcagcagccg tccggaaaac ctggccacgc gcagggttac
     9061 cagaccatcc gcagctgcat cccagggaag attccggtga gggatatagt cgagcaggtt
     9121 ttccgccggg gtttgccggc tccccgcctt ggcttcggcc atattgagaa ccacctttta
     9181 ggttctatgg ggtgaaagag cggatccgaa ccgtcccgga accggagcgg ccggcgcaaa
     9241 agcctgccaa catagcaata aattgagaaa aggtcaagca aaaaatcgcc agcacttgcc
     9301 agctcgcgtc agatgatgcg gtctgcccgc acgccgcccc ggccatgagc cgcctgagcg
     9361 ggactcagcc ccagcaaatc cgttttactt tttaattgta tttagcaccc atattctgta
     9421 tattcccatt ttaaaccaga cagttaatta tggcctttct cgacgatatc acccttggtc
     9481 aatactatcc cgctgactct tttctccatc ggctggaccc gcgcagcaaa cttttcgcgg
     9541 cgttcagtct gatgaccggg ctgctattaa cctataatat ttatattctt gcagggatgg
     9601 cgctgctttt cctgtttgcg atcaggatcg ccaggattcc ctatgcgctg gtgctgaaaa
     9661 atctgcgtcc ctttctctgg ctctttgcac tcacctggat catccatctg gtctggagtt
     9721 ccgggcgcgt cctctgggtt gtgcctggca cctcgatcac cctgaccagg gagggggccc
     9781 tgctcggcgg catctatgcg gtgcggctgg cgctgctcat tctctacgcc gccctgctca
     9841 ccctctgcac ctcgccgatc gagctgaccg acgccctcga gcgcgtctcg cggccgctga
     9901 agcggctggg cgtgccgacg cacgagttga gcctgatgct gaccctctct ctgcgcttca
     9961 tcccgaccct gcttgaggag gcgcagcgca tccgcaacgc ccagctctcg cgcggggccc
    10021 gcttcacggg ctcccttcgt catcgcgtgc gcgggctcct gccgctgatg gtgccccttt
    10081 tcgtctccgc cttccggcgg gcggacgagc tggccctggc gatggatgcc cgctgttaca
    10141 caggcggcga cggccgcacg gtctacgctc aactgcgtct gaccggggct gattgggggg
    10201 tgataacggg ttcgctcgtc attctggcgt tttgtatggg actctagagc ccgtgcgtaa
    10261 tattaaactg acactggaat atgatggttc cggcttttgc ggctggcagc tgcagccggg
    10321 gcagaggtcg gtgcagggcg agatcgaatc agccctcggc cggctgaccg gtcagaggat
    10381 cgggatcatc gccgccggac gcaccgacag cggtgtccac gccctcgggc aggtgatcaa
    10441 tttccgcacc gccagcgccc tgcctcttga ggtctttatg cgcggcacca acgccctgct
    10501 gcccggcgat gtccgcatcc tcgctgccga ggaggtcccg gaggctttcc atgcccgcta
    10561 tgacgccatc agccggctct accgctacca gatcgcaacc cgcccactcg ccatcggccg
    10621 gctctatacc tggcacctgc cgctgaggct cgaccttgcg gcgatgcagg aggctgcggc
    10681 cgccctgttg ggtgatcacg attttgtctc tttctgccag gccggttccg gactggcgca
    10741 ccaccgctgc actgtgctgc gtgcagaatg gcaggcctcg ggggagaggc tctccttcga
    10801 gattcacgcc aaccgcttcg tccacaacat ggtgcgcatc atcgtcggca ccagtgtgga
    10861 gatcggccgg ggcgccctgg cgccgtcggc tatggcgcag atcatcgccg cctcggaccg
    10921 ccgcgctgca ggacgcacgg cgccccccca cggcctgttt ctggtccagg tctgttatgg
    10981 agaaaaacaa ccccctttac aggagtgact atgaaattct ttatcgatac cgccaatctg
    11041 gaagagatca agagggccaa ggccctcggc gtcgccgacg gcgttaccac caaccccacc
    11101 ctgatcgcgc gcgaaccggg caagcctgat gagatctatc gcgccatctg tgccctgatc
    11161 gacggtccgg tctgcgccga ggtggtcagc ctcgacaccg aggggattct ccgcgaggga
    11221 cgtgaactcg ccgggatcgc caagaacatc gtcatcaaaa ttcctgtgac caaagaggga
    11281 ttgatggcgg tgaaggtgct cgagagcgag gggatccgca ccctggtgac cctcatcttc
    11341 agcccgctgc aggcgctgct cgccgccaag gcgggggcct cattcatctg ccccttcgtc
    11401 ggacggctcg acgacaccgg ccaggtcggc atggaactgg tcgagcagat tctctccatc
    11461 tacgccaatt atgagttcca gaccgagatc atcgtggcca gcatccgcaa tcccctccat
    11521 gtcctcgacg ccgccacgat gggggcccat atcgccacca tcccgctcaa ggtcatcgag
    11581 cagctggtca agcatcccct caccgatgcg ggcattcagc tgtttctcaa ggattgggag
    11641 agcgtcaagg cccgttcatg agaaggatat ccggaggccc ttcagcccgg ctcaccagca
    11701 caggatcagt caccatgacc gttcgacccc gctcctcagg ccccggcggg ggccggctcc
    11761 gtatcgccct gctctttgtg acggccatct tgtccctcac cagtgccgca gcagcgcaga
    11821 ggcagccgtg gcatgaggcg gagcgccgcg gcgccgccgt caaccgcgag gccgatatcc
    11881 aggatgtgat ttccaactcg cgccacaacg ccatcacccg cgcggttgca gaggtgagtc
    11941 cggcggtggt gggcatcaat gtcgtccagt tgcagcgttt ttaccagcgc accccttttg
    12001 atgacgatcc ttttttccgg cagttcttcc gggaccaccc ggtggagaag cgggtcaagg
    12061 gattgggctc gggttttatc atctcgcgcg agggatacgt cctcaccaat cagcatgttg
    12121 tcgcggatgc cacagagatc attgtcaccc ggaccggggg gaagcagtat atcgcccgca
    12181 aggtgggtga ggattatgtc agcgatgtcg ccatcctcaa gatcgagggg aatgatttcc
    12241 cctatgtgca gtttggcgac tcggacgatg tcgtcatcgg cgaatgggcc atcgccctcg
    12301 gggatccctt cgggcttttc gacatcggct ccaaggccac agtgacggtc ggggtgatca
    12361 gcgccacgga tcgcgatttc ggccggcagg atgacgaccg catcatggag gacatgatcc
    12421 agaccgatgc cgctattaac gccggcaaca gcggcggccc cctggtcaac tgctatggcg
    12481 aggtgatcgg gatcaatacc tggattctct cgggcaacgg caccaacatt ggtctcggct
    12541 tcgccctgcc gatcaaccgc gtcaaacgca tactggacga tctcatcaat tacggcgcag
    12601 tggagcggcg ctggtatacc ggtctgcgct acgaggcgat gtccctgatg acggcgcgcc
    12661 atctcggcct caggagcgtg cacggggtca tcgtctccga ggtggaagcg gcaagtccgg
    12721 ccgcccgcgg gggctttgag atcggcgatg tcatcatggg catcaacggc agggaagtgg
    12781 aaagcttcga ggatgtcaag cggatcatcg acggcctcga tctcaagcgg ggggataatc
    12841 tggttttcag ggtttttcgc ggccgcagat atctgaacct caccatcaag ctggaatacc
    12901 gcgccggcgg tgtgaaagga gagggccgat gatcaagcgt tatgccaggc cggagatggc
    12961 cgccatctgg agcgacgagc acaagtacga gacctggctg agggtggaga tcgccgtctg
    13021 cgaggcccag gccgagctgg gcgccatccc tgcggctgcc gtggaggcca tcaaggccag
    13081 ggccggattt tccatcgccc gtatcgagca gatcgaggcc gaggtcaggc acgacgtcat
    13141 cgccttcctg actgccctct ccgagagggt tggagatgag gcgcgccata tccatctcgg
    13201 catgacctcg tcggacctgc tcgataccgc tctggccctg aatatccagg aggcgggggc
    13261 tctcctgctc gaagggatgc gggaattccg cgccattctg gcccggcagg ctttaaagca
    13321 ccgcgacact gtttgtatag gccgcagcca cggcatccat gccgaaccca gcagcttcgg
    13381 actcaagttc gccctctggt acgacgagat ggggcgcaat ctggagcggt tggagagcgc
    13441 cctggaggcc gcgggtgtgg gcatgatctc gggtgcaatc ggaacctttg cctatatcga
    13501 tcccagggtg gaggtgagcg cctgtgcccg cctcgggctg cggcccgcgc cggtcaccac
    13561 ccagatcatc cagcgcgata tccacgccca gttccagagc gtcctcgccc tgtgcggagc
    13621 gaccctggaa aaaatcgccg tggagatccg tcatctgcag cgcaccgagg tcctggaggc
    13681 ggaagagccc tttagcgccg gtcagaaggg ctcctcggcg atgccgcaca agcgcaatcc
    13741 catcggcagc gagaatatcg ccggactcgc ccgcctgctg cgcaccaatg cccttgcggc
    13801 cctggaaaac atcgccctct ggcatgagcg ggatatcagc cactcctcgg tggaacggat
    13861 catcttcccc gacagctgca tcctgctcga ttacatgctt caccggctgg cgcgcatcgt
    13921 tgagggactg gtggtctatc cggaaagaat gatccgcaat ctgcgcctca ccgggggatt
    13981 aatcttctcc cagccggtgt tattggcttt gacaggcaag gggatgagcc gcgaatatgc
    14041 ctaccggatt gtccaggagg atgccatgcg ctgttaccat gaggacgggg atttcagaga
    14101 gatcctgctc ggggatgcgc gtgtcctgga gctcctcacc gccgcagagg ttgaatcctg
    14161 tttcaatgag caggtcggtt tgaaacacgt tgatgagatt ttccgtagag tgggtatctc
    14221 cgaatgacaa tccaactact tgctgctgcc agactgtgaa gaggagagac gcttgaaaaa
    14281 gaaaaaaatc tacgagggta aaaccaagaa ggtttacgaa ggcgaacacg aaaccgaact
    14341 catcctggag ttcaaggatg atgccgtagc actttccggc tccaagccga cccacatccg
    14401 gggcaagggg atgatgaaca agcagatctc ggtctatctt ttccggctgc tcgagagcta
    14461 ccacatcggc actcacctgg tccgcgagct ttcggataag gagatcctgg tcaagaggct
    14521 caacatgatc cccgtggtgg tgatggtgca caatgtcgtc gcgggcaagc tggtcaagag
    14581 ctacggcatg aaagaaggca aggagctcga atgcccgatc atcgagtatt acctcaagga
    14641 tgaggagcgg gatgatccga tgatcaacga ggaccacgtg gtctccttcg gccatgccac
    14701 ttctttggaa atcaaggaga tgcaccggtt ggcctccaag agtaatgcgg tgctcaaggc
    14761 attcctgcgc cgccgggaat tccagcttgt cgacctccgg ctcgaattcg gacgccacca
    14821 gaaccggctc ctcctcgccg atgagatcag cctgaatacg gtgcgcacca tcgacttgcg
    14881 ctatcccgaa ttgggcatcc tcgacggcga taatccccgc gtagcggaga accttgccca
    14941 ggtgaagagc agaatttttt cagcctgagg cggggcggat caaaccgcag caggtccggc
    15001 gaccagagca gccccaaacc ggggcgaccg gtattgatga cgataataaa ccaggagatt
    15061 gatcgtttcg ggggaggaga tgatcaaaca gtgaaatgaa tatcaagaaa aagcggacac
    15121 ccaggcagcg cacctacatc aatgtgccca atctcttcac tgcgttgaat attttctgcg
    15181 ggtttctttc ggtcctccag accatcgagg gcaactatac caccgccgcc tggctgatct
    15241 tttttgctgc agtctttgat gccctggatg gacgcatcgc ccgggcgacc ggccagagca
    15301 acgagttcgg gctgcaaatg gacagcctgg gcgacgtcat ctcctccgga ctggcgccgg
    15361 ccgtgctggt ctacaccatc cagctcaaga gcctgcaccc tccggtcgga ctgatccttg
    15421 ccttttttcc cgttctcttc gccgccttcc gcctggctcg cttcaatgtc ttcacgctct
    15481 ccgagggcaa gaagagcgac tatcttggct tgcccgcccc catggcgggg gtcacccttg
    15541 ccagctgtgt catcctctat caaagcgtgc agtgggatct gctgctgcgc atgctgatcg
    15601 gcctggtccc ggtcgtaagc ctgctcatgg ccagcaccat ccgctacgag ggcatgccgc
    15661 gcttctcgat cagggagagg ggctttaacc gcctcaagct catcctcttt ttcgtcatca
    15721 cagcgctctt tctcgtttat cccgattatg tgctctttcc cttcatgatt ttctacatcc
    15781 tggccggcat ctgccggtcg gtcttctcac tgatgcgcaa cgaagatgat ggggagatta
    15841 ccctgatccc caaagaaccc gatctgaaat cctaacggtg agggggagga ggaatccgtg
    15901 ttatccaagg acagataccg cattaaaaga aaattctcga tcgggatgat cctgatcgcc
    15961 gtggtgttgg gggctctgat tggctcgacc ctgggtgagg ccatcggggt gatcctgcca
    16021 gagggagtgg tcaaggattt tctaatcaaa tccgccagca tcggcttcag cccggtggcg
    16081 gtcaatctgg tcattctctc gttcacaatc ggcttcacct ttcacctcaa catcatcggc
    16141 gtgatcggta ttatcattgc ggcttacatc ctgcgctggt tcgcctgaac aattttactt
    16201 gcttctgatc agttggtgct ctatatttct ctattctgtt ctgggaggat ctcatgctgc
    16261 ccggtgtagg tatagacgag atgttcgtga ttgttctcgt cgccttgata ctcttcggct
    16321 ccaaacagct gccggaactg gcacggggtc ttggcaaagc catgcgcgag ctgaaaaaag
    16381 ccactgatga tgtcaagcgg gagcttgacc ttactgacga gctgcgcaaa tagtatttta
    16441 tcacagcgta tctggaggtt gttatgggcg ttggcccgac tgaatgggta attatcatcc
    16501 tcatcctggt gctgctcttt ggggcaaaaa agatccccga gctggccaga gggatggggc
    16561 aaggcctgcg cgagtttaaa aaggccgcaa atgaagtcca ggataccagc tcgcaatccg
    16621 cttcgcgggg cgaagtcggc ccgaaggaaa acaagcagga atagccgcct catccacctg
    16681 caaggataat gcttttgcat accggtagcg tcgattttca atccatcaag ccccttctcg
    16741 ccgccgggtc gatcagttgt acgagcctgg ccgaaacata tttgcagcac attgataaag
    16801 gcgctcattt gaacgccttt atttctgttt ttgcggagaa ggcgctcgaa caagcggcag
    16861 ccgttgaccg caaacggacc cggggcactg ccggccggct tgctggcctc tttcttggcg
    16921 tcaaggacaa catcgtctgg agcagcggcc cgaccacctg cgcttcgcgc atgctggcgg
    16981 gctaccagtc gcccttctcc gccaccgtgc tgaaaaggct ggccgaggag gatgccatct
    17041 ttctcggcaa gaccaacatg gatgagttcg ccatgggctc ctcgaccgag aattcggcct
    17101 atggtgcggt gcgcctgccg gccgatcccg ggcgcgtgcc cggaggctcc tcaggcggct
    17161 cggccgcggc ggttgccgcc ggcctctgca ccgccgctct cggcagtgat acggggggct
    17221 ccatccgcca acccgcctct ttctgcggtg ttgtcggcct caaaccgacc tgggggcgcg
    17281 tttcccgcta tggcctggtt gcctttgcct cttccctcga ccagatcggc cccatggggc
    17341 gttcggttgc ggactgcgcc gagctcctgc aggtcatggc ggggtatgat cccgccgatg
    17401 ccaccagcag catggcgccg gttccggact atgcggcatc cctgacgcgc gatctcaagg
    17461 ggctgcgcat cggtcttccc cgggagtttt tcccgcaggc agggatggag a
//
LOCUS       DAOPWU010000030        16813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3480_length_16813_cov_4.591956, whole genome shotgun sequence.
ACCESSION   DAOPWU010000030 DAOPWU010000000
VERSION     DAOPWU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16813
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3480_length_16813_cov_4.591956"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKM23_03160"
     CDS             complement(<1..256)
                     /locus_tag="PKM23_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90504.1"
                     /translation="MNPPDASRNGDLKPRRTILLLSMGHFANDAYPGFIAPLQPLLMQ
                     QANYGLTLAGVLTSIASITGSFMQPFFGLWVWARRLPGGTQ"
     gene            complement(282..767)
                     /locus_tag="PKM23_03165"
     CDS             complement(282..767)
                     /locus_tag="PKM23_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90505.1"
                     /translation="MTKRPFFTPATGLLLLIIGLTALVIKSAQDSLTQALLAALFFLL
                     AAGYFTARSFEGEKRWWMILPAGLSFTVAAIVLLRHFALLKSHLLWALFFCGAGLTLF
                     LYWKNRPHALPEKWARNGAILCALLALFQYLGSFRFMDHMTLVALLLFAAGILLLMRK
                     R"
     gene            complement(969..2552)
                     /locus_tag="PKM23_03170"
     CDS             complement(969..2552)
                     /locus_tag="PKM23_03170"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNY90506.1"
                     /translation="MKPRVIVLLLVSTLLLLLGSAFAHDPGSHDRGGLSGRVTDALTS
                     APIPRALIRACNEERGWGHKRSFQTLSLEDGTYSFAVLPTGRYIVWAQAAGHLPEYWQ
                     EADSLHKATAAAVDSGITTTGIDFTLGTGGSITGLVTDEADKLPVAGAIIEVHPRRSL
                     ACWNFIRSDAEGRYLVTGLEDGEYLVSAWRVGYYPQWYDSVSMRREATPVTISGHSAA
                     AGIDFALSKPAPLPRSISGVVSDDTTGLPVENARIMAIPLRSFNRPRRVLSDATGSFV
                     LRGLAAGKYVLLIDARGYKGEFYDNVRSWKEALAIEVLEGQEVTGIEIKLAPQLAGAH
                     RITGRVLDGQGQAVEGALVRMNEGVEEIAAAVTAEEGYYDLEDLPAGSYTLTASAPGY
                     EEATPAFAQLSLGSALPVDGLTLLVRSTATGIGENPQLPEAPELGQNYPNPFNPDTRI
                     DFTLPARGMVRLTLHDMLGREIRTLVHGEMAAGGHSAVWDGADDYGSRMASGIYLCRL
                     EVESGSGSFTRVRRMLLLK"
     gene            complement(2667..3662)
                     /locus_tag="PKM23_03175"
     CDS             complement(2667..3662)
                     /locus_tag="PKM23_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90507.1"
                     /translation="MKKNTPLILFLALLAGGVFAQPRSGQPVQEERLRYNQLLQRTRA
                     AVSDCDSDLARRLLRQAEEKDSAIRDLQRRNDRKALRAHYADATRLLLRALDLCSEKR
                     SSGGEEIDREFAQLEQRIDRAGSPERPRSRGGRGGDIGKIKALQSQTRRALDAGQVDL
                     ARRKMDMIRLLLNRPDEPAPSGDRSSQALRGLREEIDRLYNSPEGGSPRMRSLLQAAA
                     EQAEDAERFLQRRRIVPARAAIAAGNRLLARAASPANRRDDPSATDLQAEVTFIGEML
                     YVLESERNADPARREEIRLCRTAYQKGERALKNGQPGIAHEYFLLAKELIAELTT"
     gene            3973..5545
                     /locus_tag="PKM23_03180"
                     /pseudo
     CDS             3973..5545
                     /locus_tag="PKM23_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012231.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(5662..6261)
                     /locus_tag="PKM23_03185"
     CDS             complement(5662..6261)
                     /locus_tag="PKM23_03185"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNY90508.1"
                     /translation="MTKIVLITAAVLLVGALALLASAKKPVSTPVSAGSIHAFTVNDI
                     DGSPVKLEKYRGQVVMIVNVASKCGFTPQYEGLQKLYTDYKESGLVVLGFPANNFMKQ
                     EPGSDAEIKEFCSLNFGVTFPMFSKISVKGEDIHPLYAWLTSKQSNPEFAGDIAWNFN
                     KFLIGRDGKVIARFGSRTKPEDAELVDAVKKALAAVPAQ"
     gene            6495..7232
                     /locus_tag="PKM23_03190"
     CDS             6495..7232
                     /locus_tag="PKM23_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNY90509.1"
                     /translation="MYSKEYSMRIFETNPDGRLGLVALLDYLQDIASEHAVQLGFGRD
                     DLMKDNRMWVLSRLYAEIIEWPIWPAVLTIQTWHRGTEKLFSFRDYRVSGADGRPLAA
                     ATTSWLTIDQTTRRIQRPDENLLRGSSGGFDNALPRSAAKLEPVAESAILSPLFRVRF
                     SDLDVNLHTNNVHYLQWALDSYDPEFLMGHHPRSVEINFLAESRCGEEIVIRTAPAGE
                     EGAFGHAILRPADAAELCRLRIVWDRK"
     gene            complement(7250..9031)
                     /locus_tag="PKM23_03195"
     CDS             complement(7250..9031)
                     /locus_tag="PKM23_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY90510.1"
                     /translation="MAATAVASEKKGYNRFLLLVAGLGGLLYGVDVGIISGALPYLEA
                     TSKLNAGQLSFIVAAVLLGSVISTLFAGMLADWMGRKKLMILSGLLFVVSIPSIALAQ
                     GYGPLVLGRLLQGISAGLIGVVVPLYLAECLSASSRGKGTGIFQWLLTLGIMAAALIA
                     MYYSIRLDKIAALGDAARLFAAKDAAWRGIFWISLLPGLLFVVGSFIVAESPRWLYSR
                     GKTEAARAALLRSRSEEQTAIELREMEESLAAERKSATTANAKIKESLLKRKYVIPFL
                     LACIILACNQATGINSIIGYNATILIQAGLSDVAAHWGYVILTMVNFLITIVGVMLVD
                     KKGRKFLLSIGTAGIIASLIAVALLFNGVERQRLDVSAAVQQQVAADQSLTLSYNPAE
                     AARLLAGKGEAGAAIAGRPSSMIIIYSYGDFRTATKVIRSTDTLAKPIAIDRASSLPA
                     NKTVAFFSNPFGDLTTAKSAPLKIENALITPVPSAQRGWAIAILLFVFIAFFGVGPGI
                     CVWLALSELMPTRIRSNGMSIALLLNQAVSTFIAAVFLPTVGKHGYATMFFIFAGCTL
                     IYFITVAFFLPETKGKTLEEIEAHFSK"
     gene            complement(9047..10255)
                     /locus_tag="PKM23_03200"
     CDS             complement(9047..10255)
                     /locus_tag="PKM23_03200"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNY90511.1"
                     /translation="MQDKISAPSLEEIAAAFDLPGALSSGAPFGSGHINDTYRLDYTT
                     GEGPRSFVLQRINTAIFKDPDRLMENILRVTRHQRTKLQENGAGEIHRRVLEFFPARD
                     GRFCHVDPEGGCWRLSLLVEGTHTCDIIESPEQAFLTAAAFARFQQTLVDLPGGRLHE
                     TIPNFHNTALRFADFEKAVREDRAGRAAAVAPEIDFALQRRHIAPVLVDLMRKGAVPE
                     RVTHNDTKLNNILLDDASGEALCIIDLDTVMPGSALYDFGDMVRSSACTAAEDEADLT
                     LVGVEMSMFEALARGYLSVARVFLNPAEIQHLAFSGKLITFEQGIRFLGDYLNGDSYY
                     KIKYPEHNLVRQKPVQAGRLHRGTRGGDGGRGRRDRRITNRRSSSRRRELKGSAGGGG
                     RSHPAGHEWT"
     gene            complement(10350..11339)
                     /gene="mgrA"
                     /locus_tag="PKM23_03205"
     CDS             complement(10350..11339)
                     /gene="mgrA"
                     /locus_tag="PKM23_03205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HNY90512.1"
                     /translation="MTYLPDEKRYAGGHYRRCGRSGIKLPLISLGLWHNFGGVDLFEN
                     GRAIVRRAFDRGITHFDLANNYGPPFGSAEENFGKILAQDLRPWRDELVISTKAGYLM
                     WPGPYGEWGSRKYVLASLDQSLKRMGLDYVDIFYSHRPDPDTPLEETMGALDQAVRSG
                     KALYAGVSNYSAEMTARAAAILRDLGTPCLIHQPKYNIFNRWIEEGLTGVLEQEGIGC
                     IAFSPLAQGVLTDRYLKGIPGDSRAGKPHGFLRPEQITEESLAKVRRLNELAQRRGQS
                     LAQMALAWVLRLPQMTSSLIGASSVAQLENNLGALNHLDFSDEELREIDEIAR"
     gene            complement(11438..11833)
                     /locus_tag="PKM23_03210"
     CDS             complement(11438..11833)
                     /locus_tag="PKM23_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90513.1"
                     /translation="MNESTETPGSAAEKFARYGVADSDLAHVIVEFATPMLKHCASYA
                     AREEAISYAILAWNLSLESESGREESLHEMLAQLPAEKEPEIAALMQFLLRRKAELFA
                     DNRFLVVDYTLERRGEKMQIEMTTKYIPR"
     gene            complement(11844..14306)
                     /locus_tag="PKM23_03215"
     CDS             complement(11844..14306)
                     /locus_tag="PKM23_03215"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNY90514.1"
                     /translation="MDLPAYIAALPAPPQQPPDFFSFGHTAEDLAALRSALKSRGIAH
                     RIVHCGQPDPAEGEASAAPLVIWQELALPELLPRLEGLLNRCILPARLAGAPVILLSQ
                     PPRAAYRQAVSSAGLHRDREKLWQGLYLEQLASFWEWPAEEPLSPAPLYALDDNLDAS
                     QRAAVAHLQGPIRVLAPAGSGKTRTLINRVIHLLNQGVEPGRIVALAFNKKAADEMAA
                     RLQSRGIAAANRLHQPGVVVRTFHGFGYEIIRRALGWSYDGAGEQEQLRLLMQQAVSR
                     HHRLTPRRGQDALEPFIAALNRAKMELLSLDKMSVETEEESLPFAPVFEAFLALQKAR
                     RFCNFDDMIYLALVLLLERPGLRQELQQRFRFVLVDEFQDLNAAQILLMNLLALPHNN
                     LFVVGDDDQMIYGWRGADVRHILDFPRRYGGAVDCTLETNYRSAQAIVRHAAWLIGHN
                     RHRVAKPMHTPPEAAPGRVTFQNCASLWEQAREAVQWITAQREERQGQWSDFAILFRY
                     HAYKYISAILLDAAGIPHTPVDGRSLAQTAAGRDLHAWLALILKGDEAPAGTLARALK
                     RPNRFFPNHFINTIASLDDLAYAAESEELEDWLRNAAHNFLGDLAYLRELVVRKEQPA
                     AYLNHMTMIIGLADFYRQQKRPFMALDEAGFEVLLDVLITVAGSCSSLHAFYEWLDGA
                     QAEGARPAPAADPGGVLLSTIHAAKGREFPCVILYHFNGPGSCPEEEIEEERRVAYVG
                     ATRAGEELLVLGPAGTPGLFMSELAFDPDLAAFSDRRLADEIGRLGRKQPRARKQETE
                     AAARLQAMAEELARRSALGFHK"
     gene            complement(14306..15031)
                     /locus_tag="PKM23_03220"
     CDS             complement(14306..15031)
                     /locus_tag="PKM23_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90515.1"
                     /translation="MPRSHFDVLILLGRPASGKSEIMDFLNKTGPVERVERFHIGAID
                     MIDDFPMLWVWFEEDAILEKILDKPRVQSDKEGYFLHQYQWDLLIERISMEYKKHQRG
                     FADYHAEHTALIEFSRGSEHGGYRSAFQHLSDELLSRAAILYVNVSYEESLRKNRRRF
                     NPERPDSILEHGLPDKKLERLYKEVDWEEVTAGHTDYLPINGHQVPMVVLENEPELTN
                     DFAKLAPALEAALAKLWSLYSRK"
     gene            complement(15050..>16813)
                     /locus_tag="PKM23_03225"
     CDS             complement(15050..>16813)
                     /locus_tag="PKM23_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY90516.1"
                     /translation="PPGCVYYSVAYLNFTPTRMDPMDFVDGKPVHSRAYRWRRFLNWF
                     PLGLTYAFLYMGRYNLTVSKNALGELMTKADFGAIFGAGTLTYAFAFLINGPLTDRIG
                     GKRAMLVGSAGAALMNVAMGVLTWLLLTGGLQFNMVLWFSLLYAANMYFQSFGAVAIV
                     KTNSNWFHIRERGTFGGIFGILISLGLYFAFDWGQLIVNATVANPKELSLVQRGLRSL
                     LAVDGASGIDQTWWVFFIPAILLGLFFIIDLFVLREKPSGAGFGDFNTGDATSGDADK
                     KYSTKEIIIKVLTNPILITIAFIEFCSGVMRNGIMHWYLIFTADMGYPKDFFINAHWG
                     LLLMIAGVLGGIFAGFISDKVFGSRRGPVAALLYGILLASTLAMWPFVTSTHNSVKGG
                     MQLVIQPKQIARYAEQTPLTLEGIKQKVAEKFTIEKSDAYTWKISPKESASLTASKIA
                     DITLANYERFNKYKNKSEAMHVQVKDIGVVRGAGADSRAYILGFIVVLMSLAVIGVHG
                     MLSGTSTMDFGGTKGAATAVGLIDGFVYLGTGVQSLSLGFLTSQNWSWWPPFLVPFAV
                     IGLFLAIKIWHAFPDAKRKAH"
BASE COUNT         3121 a         4899 c         5709 g         3084 t
ORIGIN      
        1 attgggtgcc accgggcaaa cgcctggccc agacccagag gccgaagaaa ggctgcatga
       61 aggatccggt gatcgaagca atggaggtga gcactccggc gagggtgagg ccgtagtttg
      121 cctgctgcat gagcaggggc tgcagggggg caataaagcc gggataggcg tcgttggcga
      181 aatggcccat cgaaagcagc aggatggtgc ggcgaggctt gaggtcgccg tttctggagg
      241 catccggagg gttcacggca ggcagttccc ggtcatcgat ctcatctttt tctcatcagc
      301 agcaggatcc cggccgcgaa caggagcagg gcaaccaggg tcatatggtc cataaaacgg
      361 aagctcccca gatactgaaa gagggcgagg agggcgcaga ggatggcgcc gttgcgcgcc
      421 cacttttccg gcagggcgtg cgggcggttc ttccagtaga ggaagagagt caggccggcg
      481 ccgcagaaaa agagcgccca gagcagatgg cttttcagca gggcgaaatg gcgcagcagc
      541 acgatcgccg ccaccgtgaa actcaagccg gccggcagga tcatccacca gcgcttctcc
      601 ccttcgaagg aacgggcggt aaaatagccg gccgccagga ggaaaaagag ggcggccagg
      661 agggcctggg tcagggagtc ctgcgccgac ttgatgacca gcgcagtgag gccgataata
      721 agcagaagca ggcctgtggc gggggtgaaa aagggccgct tggtcatgat gaaatcctcg
      781 cttggatgga aatacccgct gccgggataa ttctgtctaa tgtacaacaa ttcaagatca
      841 tttacaagtc cgaccttatg agggctgcag cgggtaaaaa aaatggcgtt gaggccaagc
      901 ggcctcaacg ccatatcctg ctgccgcccg ctcatccgga gatccggacc ggagcggaaa
      961 caccgggctc atttgagcag gagcatcctg cgcacccggg tgaaggaacc tgacccgctc
     1021 tccacctcga gccggcagag gtagatgccc gaagccatcc tgctgccata gtcatcggcg
     1081 ccgtcccaga cggcgctgtg accgccggcc gccatttcgc cgtggaccag ggttctgatc
     1141 tcccggccga gcatgtcatg gagggtcagc cggaccatcc ccctggcggg cagcgtgaaa
     1201 tcgatgcggg tgtcgggatt aaagggattg gggtaattct gcccgagctc cggggcttcc
     1261 ggcagctggg ggttctcccc gatgccggtg gccgtgcttc tcaccagcag ggtgagaccg
     1321 tcaaccggga gggcgctgcc cagggagagc tgggcgaagg caggggtggc ctcttcatat
     1381 ccgggggcgg aggcagtcag ggtatagctg ccggcgggaa gatcctcgag atcataatag
     1441 ccctcttcgg ccgtcaccgc ggcagcgatc tcctccaccc cttcgttcat cctcaccagg
     1501 gccccttcga cggcctggcc ctgtccatcc agcacccggc cggtgatgcg gtgggcgcct
     1561 gcaagctgag gggcaagttt gatttcgatg ccggtcacct cctggccttc aaggacttca
     1621 atggccaggg cttccttcca ggagcggacg ttatcataaa attcaccctt gtagccccgg
     1681 gcatcgatga ggagcacata tttgccggcc gccagccccc gcaggacaaa gctgccggtc
     1741 gcgtcggaga ggacccggcg cggacggttg aaggagcgca gcgggatggc catgatccgg
     1801 gcgttctcga ccggcaggcc ggtggtgtcg tcgctgacca ctccggagat gctgcgcggc
     1861 agtggggcgg gcttgctcag ggcgaaatcg atgccggctg cagcggaatg gccgctgatc
     1921 gtcaccgggg tggcctcgcg gcgcatggag acactgtcgt accactgcgg atagtaaccc
     1981 acgcgccagg cgctgaccag atactccccg tcctccagtc cggtgaccag atagcggcct
     2041 tccgcatcgc tgcggataaa gttccagcag gccaggctgc ggcgggggtg gacctcgatg
     2101 atggcacccg cgacgggcag cttgtccgct tcatccgtga ccaggccggt gatggaaccg
     2161 cctgttccga gggtaaagtc gatgccggtg gtggtgatgc cgctgtcaac ggcagcggct
     2221 gtggccttgt gcagggagtc ggcctcctgc cagtactccg gcaggtggcc ggcggcctga
     2281 gcccagacaa tataacgacc ggtgggcagt acagcaaagg aataggtgcc atcctcaaga
     2341 ctgagggtct ggaaggagcg cttgtgcccc catccccgct cttcgttgca ggcccggatg
     2401 agcgcacggg gaatgggtgc cgaggtgagg gcatcggtga cccggccgga gagcccaccg
     2461 cggtcatggc tgccgggatc atgcgcgaaa gcgcttccga gcaggagcag gagggtggag
     2521 acgagcaaca atacaatcac tcttggtttc atgggatact ccttgacggc ttggttagcg
     2581 tcagaattat cacgtgctgg aaaagtgacg gcctggttga aaactgccgg cccgcccggg
     2641 cctccgacgg gtgaaccggc ctccgcttag gtggtcagct cagcgatcag ttccttggcg
     2701 agcagaaaat attcatgggc gatgccgggc tggccgtttt tgagcgccct ttcgcccttt
     2761 tgataggcgg tgcggcaaag gcggatctct tcccggcggg ccggatcggc atttcgttca
     2821 gattcgagga cgtaaagcat ctcgccgatg aaggtcacct cggcctggag atcggttgct
     2881 gaaggatcgt cgcgccggtt tgcagggctg gcggcgcggg cgaggaggcg gttgccggcg
     2941 gcgatggcgg cccgggcggg gacgattctg cggcgctgaa gaaagcgctc tgcatcctcg
     3001 gcctgctctg cggcggcctg cagcaggctg cgcatgcggg gcgatccgcc ctcaggggag
     3061 ttatagagcc tgtcaatctc ctcgcgcagc ccgcgcaggg cctggctgga acggtctccg
     3121 gagggggcgg gctcatcggg gcgattgagc aggaggcgga tcatatccat cttgcgccgg
     3181 gccagatcga cctggccggc gtcgagggcg cgccgggtct gggactgcag ggccttgatc
     3241 ttgccgatgt ctcccccccg tcccccgcgg ctgcgcggcc gctcggggga cccggcgcgg
     3301 tcgatgcgct gctcgagttg ggcgaattcc cggtcgatct cctccccgcc ggagctgcgc
     3361 ttttccgagc aaaggtccag ggcgcgcagc agcaggcgcg tggcgtcggc atagtgagcg
     3421 cgcaaggcct tgcggtcgtt ccgcctttgc aggtcgcgga tggcgctatc cttctcttcc
     3481 gcctggcgca gcaggcgccg ggcaagatcg ctgtcgcagt cggagactgc agcgcgggtg
     3541 cgctgcagca gctggttgta gcggaggcgc tcctcctgaa ccggctggcc ggaacgaggc
     3601 tgtgcaaaga cgccgccggc gagcagcgcg aggaaaagga tgaggggagt gttttttttc
     3661 atgatctctt tcacggctgg gcacaccatc tttttaagct gcactgttgc aacccctgtg
     3721 ccagggtcga agggcaggaa atagaataac aaaaacaata agataggcaa tggcagcccg
     3781 attcggcccc gggaatcctg tccctccagg tacaggattg tggttgcaag tacggatatg
     3841 tacaccatta cgtttcccgt ccggggattg tttgggttcg cgccatgcgg ctggaccgat
     3901 tttctgcgcg aaactgtttg cttttatcaa aaaaaagtgc aaattggggt tgacaacttg
     3961 aaccgcagcc tggtgctgcg gcggataatc atatcgcaag aggagagaga gtatggattt
     4021 tttaaaagcc cttggcatcg ctgatgataa ctttggggcc tccaccgggc agaagtggct
     4081 ggagagcaag gatcagggca agctggatgt tttttcgccg gcggacggca aaaagatcgc
     4141 ctcggttttc cgggcttccg cggctgacta cgacgcggtc atgcagagcg ccgcagaggc
     4201 cttcaaggtc tggcgtatgg tcccggcccc caagcggggc gagattgtcc ggcagatcgg
     4261 gatgagactg cgcgaggtga aaaagcccct gggcgaactg gtcacctacg agatgggcaa
     4321 atccctgcaa gaagggtggg gcgaggtcca ggagatgatc gatatttgcg attttgccct
     4381 gggccagtcg cggcagctct acggtttcac catgcattcg gagcgcaacc gccaccgcat
     4441 gtacgaccag taccatcccc tcggcgtggt cggtgtggtc accgccttca actttccggt
     4501 cgcggtttgg gcctggaacg ccatgatcgc agcggtttgc ggcgatgtcg tcgtctggaa
     4561 gccctcctcc aagacccccc tgaccgccat cgcagtgcaa aaggccatcc gggatgtgct
     4621 gcgcgacaat gatctgcccg aggggatctt caacctggtc atcggcagcg gagcggcagt
     4681 cggcgaacgc atgcttcagg acacgcgggt gccgctggtc tcgctgaccg gttcgaccgc
     4741 cgtcggcaag catgccgcgg aggtggtcgc ccggcgcctg ggccgcacta tcctcgagct
     4801 cggtggcaat aacgccatcc tcatcacccc ggatgccgat ctcaagataa ccatccccgc
     4861 cgtggttttt ggctcggtcg ggaccgcggg tcagcgctgc acgaccaccc gccgcctgat
     4921 catccacgag tcggtctatg acaaggtcaa ggagagcctg ctcaaggcct acgcgggact
     4981 gcgcatcggc gacccgcgcg aggaaaagaa ccatatgggc cccctggtcg acaaggctgc
     5041 cgtcgatatc tttacggccg ctctggaaaa aatcaaaagc tcgggcggca ggatcctctg
     5101 cggcggcgag gtgctcgagg gcgcgggcta cgagtccggc tgctatgtga ctccggtgat
     5161 cgccgaggtc gagaacagct gggagatcgt ccaggaagaa accttcgccc cgatcctcta
     5221 tctgatccgc tatcagggcg gggtggagaa ggccatcgcc ctccacaatg atgtgcccca
     5281 gggactctcc tcggccatct tcagcaacaa cctccgcgaa accgagctct tcctctctca
     5341 tgagggctcc gactgcggca tcgccaatgt caacatcggc acctcgggcg ccgagatcgg
     5401 cggggccttc ggcggcgaaa aggagaccgg cgggggacgc gagtcgggct ccgacgcctg
     5461 gaaggcctac atgcgccgcc agaccaacac cctcaactgg agcaccgccc tgcccctggc
     5521 tcagggcatc aaattcgata tctgaagcgg ccgctgctga tcgaaaaagc aaagcctctc
     5581 cggcgttgga gccggggagg cttttttatg tcggcttgac tctggtccag ggcacagccg
     5641 gttcccggtg acagtgcggc cttattgggc cggaactgca gccagggcct ttttgacagc
     5701 atcgaccagt tcggcatcct ccggcttggt gcggctgccg aaacgggcaa tgaccttgcc
     5761 gtcgcggccg atgaggaact tgttgaaatt ccatgcgatg tcgcccgcaa attcgggatt
     5821 gctctgtttg gaggtcagcc aggcgtagag gggatgaata tcctcgccct tgaccgaaat
     5881 cttgctgaac atcggaaagg tgaccccgaa attgagggag caaaactcct tgatctcggc
     5941 atcgctgccg ggctcctgct tcataaagtt gttggccggg aagccgagga cgaccagccc
     6001 gctctccttg tagtcggtgt agagtttctg tagcccctcg tactgcgggg tgaagccgca
     6061 tttgctcgcc acattgacga tcatgaccac ctggccgcgg tatttttcca gcttgaccgg
     6121 actgccatcg atatcgttga cggtgaatgc atggatggaa ccggccgaga ccggcgtcga
     6181 caccggtttt ttggccgagg ccagcagggc cagagccccg acaagcagaa cggcggcagt
     6241 gatgagcacg atttttgtca tatttttacc tcgcttgatg agtttcaacc tgtttgcact
     6301 gagtaataca gtttttcacg gccgcttatt ccgctgcccc ggctgcgcac ctgccgctgc
     6361 ggtgcctttt tccttgcata tggccggctg catgggtact tttttgtccg ggaggtgcgg
     6421 ccgctgcggc gcgaacggta agaccgactg atgaaaatgc ggctgcggaa ctatttacag
     6481 acagggaagc ttgcatgtac agcaaagaat acagcatgcg gattttcgag accaatccgg
     6541 acggccggct ggggctggtc gcccttctcg actatctgca ggatatcgcc tcggaacatg
     6601 cagtgcagtt ggggttcgga cgggatgacc tgatgaagga taaccggatg tgggtgctct
     6661 ctcgcctcta tgcggagatt atcgagtggc cgatctggcc ggcggtgctc accatccaga
     6721 cctggcacag ggggaccgaa aagctctttt ctttccggga ctaccgggtc tccggtgcgg
     6781 atggccgccc gctcgccgcc gccaccacct cctggctgac catcgaccag accacccgcc
     6841 gcatccagcg gccggatgaa aatttgctcc gcggcagttc cgggggattt gacaatgccc
     6901 tgccgcgcag cgctgccaag ctggagccgg tggcggaatc ggccatcctt tcgccgttgt
     6961 tccgggtccg cttcagcgac ctcgacgtca acctgcacac caacaacgtc cactatctgc
     7021 agtgggccct cgacagctac gatcccgaat tcctcatggg gcatcacccc cgctccgtgg
     7081 agatcaattt cctcgccgaa tcgcgctgcg gggaggagat cgtcatccgc acggccccgg
     7141 cgggggagga aggcgctttt ggccacgcca ttctgcgccc cgccgacgcc gccgaactct
     7201 gccggctgcg catagtctgg gaccggaaat agagccccgc ccactctcct cacttgctga
     7261 aatgggcctc gatctcctcg agggtcttgc ccttggtctc gggcaggaaa aaggcgacgg
     7321 taatgaagta gatcagggtg cagcccgcaa agatgaagaa catggtcgca taaccgtgct
     7381 tgcccaccgt cggcaaaaag accgccgcga tgaaggtgga aaccgcctgg ttgaggagca
     7441 gggcgatgct catgccgttc gagcggatgc gggttggcat cagctccgag agggccagcc
     7501 agacgcagat ccccggtccc acgccgaaaa aggcgatgaa gacgaagagc aggatcgcga
     7561 tcgcccagcc gcgctgggcc gagggcaccg gtgtgatcag tgcgttttcg atcttgaggg
     7621 gggcgctctt ggccgtggtc agatcgccga agggattgct gaaaaaggcg acggtcttgt
     7681 tggccggcag gctcgaggcg cggtcgatgg cgatcggctt ggcgagggtg tcggtcgagc
     7741 ggatcacctt ggtggcggtg cgaaaatccc cgtaggagta gatgatgatc atgctgcttg
     7801 gccgtccggc gatggccgca ccggcctcgc cctttccagc cagcagccgc gccgcctcgg
     7861 cgggattgta gctcagggtc agactctgat ccgccgccac ctgctgctgg accgcagcgc
     7921 tgacatcgag gcgctggcgc tcgaccccgt tgaaaagcag ggcgacggcg atcagcgagg
     7981 cgatgatgcc ggcagtgccg atcgagagca ggaatttgcg tcccttttta tcgaccagca
     8041 tcaccccgac gatggtgatg aggaaattga ccatggtgag gatgacatag ccccagtggg
     8101 ccgcgacatc ggagaggccg gcctggatga ggatggtcgc gttatagccg atgatcgagt
     8161 tgatgccggt ggcctggttg caggcgagga tgatgcaggc gagcaggaag ggaatgacgt
     8221 atttgcgctt gagcagggat tccttgattt tcgcgttcgc agtggtggct gatttccgtt
     8281 ccgcggccag gctctcctcc atctcgcgca gctcgatggc ggtctgctcc tcgctgcgcg
     8341 aacgcagcag ggcggcgcgg gcggcctcgg ttttaccgcg gctgtagagc cagcgcggcg
     8401 actcggcgac gatgaagctg ccgaccacga agagaaggcc ggggagcagc gagatccaga
     8461 agatgccgcg ccaggcggcg tccttggcag cgaaaagccg ggcggcatcg ccaagggcgg
     8521 cgatcttgtc cagccggatg ctgtaataca tcgcaatcag ggccgccgcc atgatgccga
     8581 gggtgagcag ccactggaag atcccggtcc ccttgccgcg cgacgaggcg ctcagacact
     8641 cggccaggta gagggggacg accacgccga tgaggccggc gctgatcccc tgcagcagcc
     8701 tcccgagcac gagggggccg tagccctggg cgagggcgat cgaggggatg ctgaccacga
     8761 agagcagtcc gctcaggatc atcagctttt tacgccccat ccagtcggcg agcatgccgg
     8821 cgaaaagggt ggagatgacg ctgccgagga gcaccgcggc aacgatgaag gagagctgcc
     8881 cggcattcag tttggaggtg gcctccagat agggcagcgc cccggagatg atgccgacat
     8941 caaccccgta gagcagtccc cccagtcctg cgaccaggag caggaaacgg ttatacccct
     9001 tcttttccga tgcgaccgca gttgccgcca tttccgatcc tttcaattag gtccattcat
     9061 gtcctgcagg atgactgcgc ccgccaccgc ctgcggaacc cttgagctct cgcctgcggc
     9121 tggaggagcg ccgatttgtt atccggcgat ctcgtcgacc acggccgcca tctccgcctc
     9181 gtgttcctcg atggaggcga ccagcctgaa ctggtttctg gcggacgagg ttgtgttccg
     9241 gatatttgat cttgtaatag ctgtccccgt tgagataatc gcccaggaag cggatgccct
     9301 gttcgaaggt gatcagcttg ccgctgaagg cgaggtgttg tatttcggcg gggttgagaa
     9361 agacccgggc caccgagagg tagccccggg cgagggcctc gaacatgctc atttccaccc
     9421 cgacgagggt caggtcggcc tcgtcttcgg ccgcggtgca ggcggagctg cgcaccatgt
     9481 cgccgaaatc gtagagggcg ctgcccggca tgacggtgtc gaggtcgatg atgcagaggg
     9541 cctctccgct ggcatcgtcg aggaggatgt tgttgagctt ggtgtcgttg tgggtgacgc
     9601 gttcgggtac ggcccccttg cgcataagat cgaccagaac cggcgcgata tgacggcgct
     9661 gcagggcgaa atcgatctcc ggggcgacgg cggcggcgcg accggcgcgg tcctcccgca
     9721 ccgctttttc gaaatcggcg aagcggagcg ccgtgttgtg aaagttgggg atggtctcat
     9781 gcagccggcc gcccggcagg tcgaccaggg tctgctggaa acgggcgaag gcggcggcgg
     9841 tgagaaaggc ctgctcgggc gactcgatga tgtcgcaggt atgcgtgccc tcgacgagga
     9901 gggagaggcg ccagcagccg ccctcggggt cgacgtggca gaaacggccg tcgcgggcgg
     9961 ggaaaaattc cagcacacgg cggtggattt cgccggctcc gttctcctgc agtttggtgc
    10021 gctggtggcg ggtgacgcgg aggatattct ccatcagccg gtcaggatct ttgaagatgg
    10081 cggtgttgat gcgctgcagc acaaagctgc gcgggccctc ccctgtggta tagtcgaggc
    10141 ggtaggtgtc gttgatgtgg ccggagccga agggggcgcc gctgctgaga gcgccgggca
    10201 ggtcaaaggc cgcggcgatc tcttcaagtg agggtgctga tattttatcc tgcatggagg
    10261 gtggattcct ttcctttggc aggccaatat aggcattggg gaccattcaa atcaattaaa
    10321 atttgtatgc gccggtacga gtccgaaact caccgtgcga tttcgtcgat ctcgcgcagt
    10381 tcctcgtcgc tgaaatccag atggttcagg gcgccgaggt tgttctccag ctgggccacg
    10441 ctgctggcgc cgatcaggga cgaggtcatc tgcggcaggc gcagcaccca ggccagggcc
    10501 atctgggcca gggactggcc gcggcgctgg gccagctcgt tgaggcgccg tactttggcc
    10561 aggctctcct cggtgatctg ctcgggccgc aggaagccgt gcggcttgcc ggcccgggaa
    10621 tctccgggaa tgcccttcag gtagcgatcg gtgagcaccc cctgggccag aggcgaaaag
    10681 gcgatgcagc cgatgccctc ctgctcgagc acaccggtca gaccctcctc gatccagcgg
    10741 ttgaagatat tgtatttggg ctgatggatg aggcaggggg tgccgaggtc gcgcaggatc
    10801 gcggccgccc gggcggtcat ttcggcggaa tagttggaaa ccccggcgta gagggccttg
    10861 ccgctgcgca ctgcctggtc gagggcgccc atggtctcct cgagcggggt gtcgggatcg
    10921 ggacggtggc tatagaaaat atcgacatag tcaaggccca tgcgcttcag gctctggtcg
    10981 aggctggcga gcacatactt gcgcgaaccc cactcgccgt aagggcccgg ccacatcaga
    11041 tagccggcct tggtggagat gaccagttca tcgcgccagg gccgcaagtc ctgggcgagg
    11101 atcttgccga aattctcctc ggccgagccg aagggggggc catagttgtt ggcaagatcg
    11161 aaatgggtta tgccacggtc gaaggcgcgg cggacgatag cgcggccgtt ctcgaagaga
    11221 tcgaccccgc cgaagttgtg ccacaacccc agggagatga gggggagttt gattccgctg
    11281 cggccgcagc ggcggtagtg gccaccggcg tagcgttttt catcgggaag ataggtcatg
    11341 gaaactcctc atttttggcg cagtttttcc ggatcagccg gggatatccg cggcagcagg
    11401 gtggggtcaa gccgcttcac cacttcgacc gatccggcta tctcggaata tattttgtgg
    11461 tcatctcgat ctgcattttc tcaccccggc gctccagggt gtaatccacc accaggaaac
    11521 ggttgtcggc gaaaagctct gccttgcgcc gcagcagaaa ctgcatcagg gccgcgatct
    11581 ccggctcttt ttcggcgggg agctgcgcca gcatctcatg cagggactct tcccggcccg
    11641 attcgctttc gagggagaga ttccaggcga ggatggcata gctgatcgcc tcctcccgcg
    11701 ccgcatagct cgcgcagtgc ttcagcatcg gtgtggcgaa ctcgacgatg acgtgcgcga
    11761 ggtccgagtc agccacgccg tagcgggcga atttctccgc ggccgatccg ggtgtttcgg
    11821 ttgattcgtt catggattcc tttttattta tgaaaaccca gggcgctgcg ccgggccagt
    11881 tcctctgcca tcgcctgcag ccgcgccgcc gcctccgttt cctgttttct cgcccgcggc
    11941 tgcttgcggc cgaggcgccc aatctcgtcg gccaggcggc ggtccgaaaa agcggccaga
    12001 tcggggtcga aggccagctc gctcataaag agcccgggcg tgccggcggg gccgaggacg
    12061 agcagttcct ccccggcgcg ggtcgccccg acataggcga cgcggcgctc ctcctcgatc
    12121 tcctcctcgg ggcaggagcc cgggccgtta aaatggtaaa ggatgacgca ggggaactcc
    12181 cgccccttgg cggcatggat ggtcgagagc agcacaccgc ccggatcggc cgccggcgca
    12241 ggccgggccc cctcagcctg ggcgccgtcg agccattcgt aaaaggcatg cagtgacgaa
    12301 caggaacccg ccaccgtgat aaggacatcc aggagcacct cgaaccccgc ctcatccagg
    12361 gccataaagg ggcgcttttg ctggcggtag aaatcggcaa ggccgatgat catggtcatg
    12421 tggttcagat aggcggcggg ctgctccttg cgcaccacca gctcgcgcag ataggcgagg
    12481 tcgccgagga aattgtgcgc cgcattgcgc agccaatcct ccagctcctc cgactccgcg
    12541 gcataggcga gatcatccag gctggcgatg gtgttgataa agtggttggg aaagaaccgg
    12601 ttgggacgtt tgagcgcccg cgccagcgtt ccggcgggag cctcatcccc cttgaggatg
    12661 agggcgagcc aggcatggag gtcgcggccg gcggccgtct gggccaggct gcggccgtcg
    12721 acgggggtat gcggaatccc ggcggcatcg aggaggatgg cagagatata tttgtaggcg
    12781 tggtagcgga agaggatggc gaaatcgctc cactgccctt gccgttcttc gcgctgcgcg
    12841 gtgatccact gcaccgcttc ccgggcctgc tcccagaggg aagcgcagtt ttgaaaggtg
    12901 acccgccctg gcgcggcctc tgggggcgtg tgcatcggtt tggcgacgcg atggcggttg
    12961 tggccgatca gccaggcggc gtggcgcacg atggcctgcg cggaacgata attggtctcg
    13021 agggtgcagt cgaccgcccc gccgtaacgc cggggaaaat cgaggatgtg gcgcacgtcc
    13081 gctccgcgcc agccgtagat catctgatcg tcgtccccga ccacgaagag attgttgtgc
    13141 ggcagggcga gcaggttcat cagcaaaatc tgggcggcgt tgaggtcctg gaattcatcg
    13201 accaggacga agcggaagcg ctgctgcagc tcctggcgca gccccgggcg ctctagcagc
    13261 agcaccaggg cgaggtagat catgtcgtcg aaattgcaaa accgccgtgc cttttgcagg
    13321 gcgagaaagg cctcaaagac gggggcgaag gggaggctct cctcctcggt ctccacgctc
    13381 attttgtcga gggagagcag ctccatcttg gcgcggttga gggcggcgat gaagggctcg
    13441 agtgcgtcct ggccgcgccg cggggtcagc cggtggtggc gggagaccgc ctgctgcatg
    13501 agcaggcgca gctgctcctg ttcgccggcg ccatcatagc tccagcccag ggcgcggcgg
    13561 atgatttcat agccgaagcc gtgaaaggtg cgcaccacca cgccgggctg atgcagccgg
    13621 ttggccgcag cgatgccccg gctctgcagc cgcgccgcca tttcgtcggc agcctttttg
    13681 ttaaaggcga gggcgacgat ccggcccggc tccaccccct ggttgaggag atggatgacg
    13741 cggttgatca gggtgcgggt cttgcccgaa cccgcggggg ccagcacccg gatcggcccc
    13801 tgcagatggg cgacggcggc gcgctggctg gcatcgagat tatcgtcgag ggcgtagagg
    13861 ggggcggggg agaggggctc ctccgccggc cactcccaga agctggcgag ctgctccaga
    13921 tagagcccct gccagagctt ctcgcggtcg cggtgcagtc cggcgctgct gaccgcctgg
    13981 cggtaggccg cgcgcggcgg ctgcgagagc aggatcactg gcgcccccgc cagccgggcg
    14041 ggcaggatac agcggttgag cagcccctcg aggcgcggca gcagctccgg cagcgccagc
    14101 tcctgccaga tcaccagggg ggctgcactg gcctcgcctt ccgcaggatc aggctgaccg
    14161 cagtgaacga tgcgatgggc gatcccccgg ctcttgaggg cggagcgcag cgccgccaga
    14221 tcttccgccg tgtggccaaa gctgaagaaa tccgggggct gctgcggcgg cgccggcagg
    14281 gcggcgatgt acgccggaag atccattatt tgcgggagta gaggctccat aatttagcca
    14341 gcgcggcctc gagggccgga gccagtttgg caaaatcgtt ggtcagctcc ggctcgttct
    14401 ccagcaccac catcggcacc tggtgcccgt tgatgggcag gtaatcggta tggccggcgg
    14461 tcacctcctc ccagtcgacc tccttgtaaa gccgctccag ctttttgtcg ggcaggccgt
    14521 gctcgaggat gctgtcgggg cgctcgggat tgaagcggcg gcggttcttg cgcagcgact
    14581 cctcatagct gacattgacg tagaggatgg cggcgcggga gagcagctca tcggagaggt
    14641 gctgaaaggc ggagcggtag ccgccgtgct cgctgccgcg cgagaactcg atcagggcgg
    14701 tgtgctcggc gtgatagtcg gcgaacccgc gctggtgctt cttgtactcc atggagatgc
    14761 gctcgatgag caggtcccac tggtactggt ggaggaaata gccctccttg tcgctctgca
    14821 cgcgcggttt gtcgaggatt ttttcaagaa tggcgtcctc ctcgaaccag acccagagca
    14881 tgggaaaatc atcgatcatg tcgattgcgc cgatatggaa acgttcgacc cgctcaaccg
    14941 ggccggtctt gttgagaaaa tccatgatct cggatttgcc cgaggcgggg cggccgagca
    15001 ggatgagaac gtcgaaatgg ctgcggggca tgagggctcc tttgctgggt taatgggctt
    15061 tgcgtttggc gtccgggaag gcatgccaga ttttgatggc caggaacagt ccgatgacgg
    15121 cgaagggcac caggaagggt ggccaccagc tccagttctg gctggtgagg aagccgaggg
    15181 agagggactg caccccggtg ccgaggtaga caaagccgtc gatcagtccc accgccgtgg
    15241 ccgccccctt ggtgccgccg aaatccatgg tcgaggtgcc ggagagcatg ccgtggacgc
    15301 cgatgacggc cagggacatc aggacgacga tgaagccgag gatataggcg cggctgtcgg
    15361 ccccggcgcc gcgcaccacc ccgatatcct tgacctggac gtgcatcgcc tcactcttgt
    15421 tcttgtattt gttaaaacgc tcgtaattgg ccagggtgat atcggcgatc ttgctggcgg
    15481 tcaggctggc ggattctttg gggctgatct tccaggtgta ggcatcgctt ttttcgatgg
    15541 tgaatttctc cgccaccttt tgtttgatcc cctcgagggt gaggggggtc tgttcggcat
    15601 agcgggcgat ctgcttgggc tgaatcacca gctgcatgcc ccccttgacc gaattgtggg
    15661 tcgaggtgac gaagggccac atcgccaggg tgctggccag gaggatgccg tagagcaggg
    15721 cggccaccgg cccgcgccgg gagccgaaaa ccttgtcgga gataaagccg gcgaagatgc
    15781 caccgaggac cccggcgatc atcagtaaaa gtccccaatg ggcgttgatg aaaaagtcct
    15841 tgggatagcc catatccgcg gtgaagatca gataccagtg catgatgccg ttgcgcatca
    15901 ccccggagca gaactcgatg aaggcgatgg tgatcaggat cggattggtg aggaccttga
    15961 tgatgatctc tttggtggaa tactttttat ccgcatcgcc gctggtggcg tcgccggtgt
    16021 tgaaatcgcc aaagccagcg ccggagggct tttcccgcag gacgaacagg tcgatgatga
    16081 aaaagaggcc caggaggatg gccgggatga aaaagaccca ccaggtctgg tcgatgccgc
    16141 tggcgccgtc gacggccagc aggctgcgca ggccgcgctg caccagggag agctccttgg
    16201 gattggccac ggtggcgttg acgatgagct ggccccagtc aaaggcgaaa tagaggccga
    16261 gcgaaatgag gatgccgaag atgccgccga aggtgccgcg ttcgcggatg tggaaccagt
    16321 tggagttggt cttgacgatg gcgacggcgc cgaagctctg gaagtacatg ttggcggcgt
    16381 agaggagcga aaaccagagc accatgttga actgcaggcc gccggtgagg aggagccagg
    16441 tgagcacccc catggcgacg ttcatcaagg ccgctccggc cgagccgacg agcatggcgc
    16501 gtttgccgcc gatgcggtcg gtgagggggc cgttgatgag aaaggcaaag gcataggtca
    16561 gggtgccggc cccgaagatg gcgccgaaat cggccttggt catgagctcg ccgagggcgt
    16621 tcttggagac ggtgaggttg tagcgcccca tgtagagaaa ggcgtaggtg aggccgaggg
    16681 gaaaccagtt gagaaagcgg cgccagcggt aggcgcggct gtgtacgggc ttgccgtcaa
    16741 cgaaatccat aggatccatc cttgtagggg tgaaattcag gtaagcgacg gaataatata
    16801 cgcagccggg ggg
//
LOCUS       DAOPWU010000031        16560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3543_length_16560_cov_4.916631, whole genome shotgun sequence.
ACCESSION   DAOPWU010000031 DAOPWU010000000
VERSION     DAOPWU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16560
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3543_length_16560_cov_4.916631"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKM23_03230"
     CDS             complement(<1..400)
                     /locus_tag="PKM23_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNY90517.1"
                     /translation="MMRLNANPFLAPEPEFTAYESAAVAVLPFPYEGGVSYGHGTGAA
                     PRAILEASAYVEFYDEVLKAEPYRMGITTLAAPAIPKSPEGMIKTLYEKSSALLRDDK
                     FIVVLGGDHSISSGFFRALLEKYGRLSCIQL"
     gene            complement(397..1467)
                     /locus_tag="PKM23_03235"
     CDS             complement(397..1467)
                     /locus_tag="PKM23_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNY90518.1"
                     /translation="MHLQRPSYFHDAPDIIPTRVKRNQTISDLVEALGATSFEARNVY
                     RGANLFKRMVHDGDTIWLGIAGAGIAGGMGGMVISLLEAGFIDIICSTGAQVYHDLHF
                     AFGLPVKAISPLANDDELRAAGDTRIYDIGIREKETLEAQDELIRRFIVERYDRLKGK
                     ALSSWEFNYEMGLWVAETAPHPELSFVAVAARAGVPIFWDSLSNHSIAMNLARTDREG
                     YPVVLSGQRDIFDSAAISYGANGTGFVELGGGGPKNFIQQTGPTISQILGVEFEGADR
                     GLQIGTAVEREGSLSSCTFSEAVTWGKYRSTGDDKLVQIWGEYSVIFPILTAFVLDQC
                     PNRPHKQIVGHMPALIEKLAGA"
     gene            complement(1593..2141)
                     /locus_tag="PKM23_03240"
     CDS             complement(1593..2141)
                     /locus_tag="PKM23_03240"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNY90519.1"
                     /translation="MVPKRMFFTKGVGYHKNKLQSFELALRDADIEICNLVTVSSIFP
                     PDCKIISRAQGIKLLSPGQITFVVMAREATCEPNRLVSAAIGLATPKDSNQYGYLSEH
                     HGFGETMKKSADFAEDLAATMLASTLGIELDPEVAWDERKKEYKVDQKNNFVSRSICQ
                     SAEGHKVGLWTTVLAAAVLIME"
     gene            complement(2255..3001)
                     /locus_tag="PKM23_03245"
     CDS             complement(2255..3001)
                     /locus_tag="PKM23_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90520.1"
                     /translation="MLIVLLLLAAAEGTPASPADADSTGEEQPSWRGGVEMAMTNNYL
                     WHGLSVNKGAILQPAAWLTLNGVTFYLWSSWTLSNPANDIKRSEIEAALIHEFSLENL
                     DFEVYFNYYKYIDQPDDPDTGEAALIISRPFGLVRTKLSLFCDVVEYPGALYAEPALE
                     VEKEFSEAWSAFAAVTLGLGSKKFNTVYCGLESATINMLSLDGRVSRTLTSGLSLQPW
                     FQYNFTLNHELKNSLGKWNGCIGFTAGKEL"
     gene            complement(3099..5834)
                     /gene="uvrA"
                     /locus_tag="PKM23_03250"
     CDS             complement(3099..5834)
                     /gene="uvrA"
                     /locus_tag="PKM23_03250"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNY90521.1"
                     /translation="MSNAIEIIGARTHNLKNISVTIPRNQLVVITGVSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLERMERPDVDQIYGISPAVAIEQKNTSQSSRSTVGTA
                     TEIHDYLRLLYARIGRAFCPSCGEPVRREEVSDVVEHLHGLPSGTRCIITFPHPADAA
                     SLESLRVKGYHRYLDGGRTLSFDEAPPEGERIDVIVDRLITAAEISSRVADSAHLAFE
                     MGKGHLDLHFSEGPPQPFSEGYECARCGIVLPEPQPGLFSFNHATGACPVCNGFGDII
                     TIDEELVVPNPEKSIAAGCIAPWSTPSHRELHEQLMHIAPRHGIDIYAPYAELSPEAR
                     EIIWKGVDDFPGIAAFFGWLESKKYKIGVRVFLSRYRGYVTCAACKGARLRPEALNFR
                     INEATIADLNRKSLQEVLHFFRTIELSPFESAIAGQILKELLSRLSYIDTIGMGYLTL
                     DRRTGTLSGGEFQRINLATALGAQLVGSLYILDEPTIGLHPRDSAKLVEILKALRDIG
                     NTVVVVEHDREMMRQADQIIDLGPGAGENGGEVIFSGSFDEIRIDGRSLTGAYLRGNR
                     MIPLPDRRRPLRAPGLQIRGAREHNLKNIDVDIPLGGMVVITGVSGSGKSTLVHDVLY
                     AGVMKKKGGWKKRVGRFRSLSGEELIDEVVMVDQSPIGRTPRSNPATYVKAFDPIRQL
                     FAETHRARVRGLKSGAFSFNVPGGRCETCEGAGVVRVEMQFLADLLLECDSCGGKRYR
                     RDILDIQYRGRNISEVLNMTVNEALDFFDHEHAVTKRLKLLKEVGLGYLKLGQPATTL
                     SGGEAQRVKLAAHLGDPKRKQVFYIFDEPTTGLHFDDVATLLHCFNALIAAGHSVLVI
                     EHNLDVIKCADYIIDLGPEGGEAGGYVVTRGTPEEVAATSASFTAHYLKPLLVKS"
     gene            complement(6039..6575)
                     /locus_tag="PKM23_03255"
     CDS             complement(6039..6575)
                     /locus_tag="PKM23_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNY90522.1"
                     /translation="MHGKTARLTRMVLLIAIALIVQMIGLPQPFTGPLVNLMLALTTL
                     ITGTTGGIALGCLTPLIALMRGQLPPLLAPFVPFILAGNALYVLFLGLFCKKGCGLPL
                     RSWRAWLGVLLGSIAKFLWLFAAARLIMPLLLARTLPEPVIAMLTFPQLITALIGGAL
                     ALLFHALLVRRRILTAPA"
     gene            complement(6585..7865)
                     /locus_tag="PKM23_03260"
     CDS             complement(6585..7865)
                     /locus_tag="PKM23_03260"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNY90523.1"
                     /translation="MQPDYKKQRRQLEQTRAQLAGLQSALLERLFTLYRDHGLIRKRA
                     ELQDPAVRQQCLSHPFDSVTRSEVEVIVSQRRPLGEGMARLKTEIDQLASLEEKERRV
                     VYDLPPAPKPTPQQISRSYGAPELKRYRQKHMSWALGIASIIAFCGVGIYWLTVYLQP
                     AELIEKKVRITKYIELEPPPSIADDLNQPAGGDNLLGGSTSGIIGVLGVIVPVSDKDK
                     KNNGIEFLVSDNSIQDLDRLLSQAKLRTGSGGGTYGEGGLGLGSGRGSGRGRSSADQA
                     EDQVVLDEFNVAALGNVDQLITEAKGVETVKLEKRGQVNIQAPGEIRGSETARVQRSA
                     ESVMGVINGQQARMMYIYNKHLRINPDMRGKLNIDLTIEADGAVSNIAVVESNIGSEE
                     FVRELLGLLRRLRFEKITEGSVTVNLPLVFNRTE"
     gene            complement(7884..8381)
                     /locus_tag="PKM23_03265"
     CDS             complement(7884..8381)
                     /locus_tag="PKM23_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90524.1"
                     /translation="MSRRTTLPIVLLLPALLLAASFGTADAQERPFDITDTTAVLSAP
                     RTGEQNPDRDGTALFNDLSTEASSTGAQDSLRLLAPRTSLPDSLREDEESFKLFLDKI
                     EVEGKLEKPQAIFIIPGSDPEIDDIQIQRSFFEEIFRVVEPAGRIGLRQKPSSTSKRK
                     DVFPW"
     gene            complement(8378..11305)
                     /locus_tag="PKM23_03270"
     CDS             complement(8378..11305)
                     /locus_tag="PKM23_03270"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY90525.1"
                     /translation="MPLQPLLLLACFLFLPVTGPAIGSQIRNNGQIVEKVVQASGEDL
                     EKLNRIILEHEELLQKYPDNDFTPTVLLQLAQLYQRHSAALFQQQMEQYEKQLEAFEK
                     NEILDEPEIPRTNMEKSISLLEQLVEKYPGVSFRDKALYMLGMAYLQQGNRVRAQLCL
                     ERIISEFPTSAITLESHFRIAEFYFDRREYQPAIEHYKPLLEKWDNPYFDMALYKLGW
                     SYYSLGDYPHAITSFIYLLEDMALIERSDSQQLNRSRADLSSEAIQYIASCYAEYGGP
                     AAAQSFFASRTDKSYTLPILVQLAALYQKRAYYPEAIAAQEVLLTLYPFYENAPEVLQ
                     QMVDNYEADSRPAQALLTRERIVQQFAPGGFWLLQHASGPVHVKADSLARNTLRGLGI
                     HHQAEAQRNGRVRDYQIAIEKYEDYLEKYGAAADAAEIHYFLAECNYGRADYAAAITA
                     YTAVLTQYDTTRYRHDAAYNRVLCHYQLLGSDQGGDSAAVAIPDFIGSGDTLRLAVSR
                     PSESGLLLAANEFIRLFPESKNHDQVLMKLGETLHEMHHYAAAARVYKHVVDLGPDRP
                     YYLSAALNAGQCYFDDNRFEEADRWFTSLQEQFPDSAHYRDRAGRLGTLARFKIAEKL
                     AAGGRSEESARLMQSVAMAANETALRDRALFEAAAQFQKLKNNREAAQALEQLAAQQP
                     PSELADEALYRAAVLRETEGEWQLAVHDYLLLCDTWPESNYAQRSLKSAAICQENLQN
                     WAEAERLYSRFALTYPDSLTDLVESLAKAGEMAQKTGDSAGARRYYTRAVASWRENGQ
                     AAADIDPYYVAQAQFMLGEMLFSEYEQISLQPPFERSMKRKVAKFNEVFEAYKETLEF
                     QIADWSTAASYRIGASFEEFVRAFMTSPPPRGLKGEALQQYQDKLVEAAKPYKERALE
                     TYAKMVEQARANAIENNWVSRSRERLQALQIELGVAPAAEPPPPANTEETTPANQEGA
                     TPS"
     gene            complement(11394..12722)
                     /locus_tag="PKM23_03275"
     CDS             complement(11394..12722)
                     /locus_tag="PKM23_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY90526.1"
                     /translation="MHLALTVLLLALMATTSLPAPAAAQATSEWRKSSERLLSQARRH
                     YLSENYWEAARDLIILLDFNPGFEKSDEVVFSLAECLYQIGLPQGAERLYKNLVTQYL
                     RSPLQPNALLGLERIEYDRKEWNRCIEYHQIIIRSASPQAVTDASNYFSGLAYYQLRD
                     FPNSKQSLVRVSIKSPWYPFAQYNLALAQMRMKQVPDAVMTLRAICDLPVESEQIRTL
                     IDDTRMTLGYLFYELERYQQAHDQFQLVSPTHLHRDEALLAAGWCQVQMGRLSTAIKP
                     LTTLLIHYPGSASNEEGLFLLGRCFMKLQRYDHAIRVYDHLISMFPSREEMPVISREV
                     QMTLLDEGIEVEKLQTDLLIMESELIDALPLQVTWPEMPDELREQRQQLLDLRQGLLR
                     RIQEERDRVNQLADGMEELRRMTRRKQSRMDWRAYAEFGKSRALFMQSMQ"
     gene            complement(12808..13878)
                     /locus_tag="PKM23_03280"
     CDS             complement(12808..13878)
                     /locus_tag="PKM23_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90527.1"
                     /translation="MQPLKSILLLAIIPVILLAPTAGLTQVTLSGGKGQLRALDADPV
                     PTGQLYINGLYSLYLEKITTPVPVGAGIGEREELAVDHTLNIALTLGLSRSFELMLHA
                     VPYQDNQRDLFGPIGDTRIGVKFHLPNRGSIFQTGLLGYVRLPTAPMHNIPYEGYSAD
                     AYSWGLTGLASLDLRSSSLALPVKFLANIGYQDQDWSDRYFSADYDQLLVALGFKFPI
                     RATLLYVETSGEIFINQGESLSLRQNLLRFSSGLRFLGPAQLVFDVAADVRLGNYTPS
                     AAEMAANPFLKRYADWKVLFGVTHRIQLFEQATEQDRVRSERRAEEEKKNEEIRKQRE
                     QVNRELEELRKKIENSPGQPPQ"
     gene            complement(14033..16228)
                     /locus_tag="PKM23_03285"
     CDS             complement(14033..16228)
                     /locus_tag="PKM23_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNY90528.1"
                     /translation="MSFSPDSFRAMIAGSPLYLALAALAAVAIALFVYRRTNPVVQPA
                     VRIVLTALRSLALLLIILALFEWTVQFRRVQTTPPLLAVAVDLSASMQQSEGGISRSE
                     RTMRLLREALPAQLDRGMATRYFGFAAHARELSPDALDSLTFNGDATDITGALEKIRS
                     SLVEQNLAGILLLSDGNYTQGGDPGRYAAEIGVPIHAIGLGSAEVPADIGITHVEANP
                     FAFAGESTPISVTVRSTSSRRENISLSLEGASGRESAARIDLQRGPLDSAIVLNYTPA
                     APGRQKLQLLLEPAGMDANRANNRFTLYQDVLRSKTLIFVLAGSLSPDLSLLRSHLAA
                     DERFDLKLLAELGDNGDLQLDRTRALQDSIGESDLLVLYNFPRRTSSPRTLSLLQAAL
                     ARRPRPLLFISGRDTDWSRVKPLEPFLPIRASVVTTGEMEVTPLLTAAGQQHPVMQIP
                     GSGTAAWNLLPPVYCREQLRGWWPDAEILAHARPAGLAPPGSGAEEWPFILVRSAGAK
                     SAAVMGYELWRWHLMMSGIGNTDETYRHFFQNVIRWLQIEQSSDLIRVRTDQSTYHFG
                     DEVRLVAQIVDARFQPVDDAEVELGITSGDKGDVQNTLYLTLTGKGSYSGAFRPEKAG
                     DYRVHARIRRNGALLGEATHLFSVGSYNEELSDVALKESLLRRLGQLSGGSYAPSDSA
                     AALIAAIQGASLHRTLTNDVELWNRGALLAAILLLLALEWFLRRRKGML"
     gene            complement(16371..>16560)
                     /locus_tag="PKM23_03290"
     CDS             complement(16371..>16560)
                     /locus_tag="PKM23_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNY90529.1"
                     /translation="MRNGPLRKIFAARCGELGIALAIPAPELCTDNAAMIAQAGRQRL
                     LAGERSDFSLDVEPNLSL"
BASE COUNT         3001 a         4592 c         5722 g         3245 t
ORIGIN      
        1 cgagctggat gcaggagagg cggccgtact tttcgagcag ggccctgaag aagccgctgg
       61 agatggaatg atccccgccg agaacaacga tgaacttgtc gtccctgagc agagcgctgc
      121 ttttttcgta gagggttttg atcatcccct cgggactttt tggaatagcc ggtgcggcaa
      181 gggtggtgat gcccatgcga tagggttcgg ccttgaggac ctcgtcgtag aattcgacat
      241 aggccgaggc ctcgaggatg gcacgggggg cggctccggt gccgtggccg taggagaccc
      301 cgccctcata ggggaagggg aggacggcca cggctgcgga ctcgtaggcg gtgaattccg
      361 gctcgggagc gagaaagggg ttggcattca acctcatcat gcgcccgcca gtttttcaat
      421 cagggccggc atatggccga caatctgctt gtgggggcgg ttcggacact ggtcgaggac
      481 gaaggcggtc aggatcggga agatgacgga gtattcaccc cagatctgca ccagcttgtc
      541 atcgccggtg gagcggtatt tgccccaggt gaccgcctcg ctgaaggtgc aggaggagag
      601 gctaccctcg cgttcgactg cggtgccaat ctgcaagccg cggtcggccc cctcgaattc
      661 gacgccgagg atctggctga tggtgggccc ggtctgctgg atgaaatttt tgggtccgcc
      721 gccgcccagt tcgacaaaac cggtgccgtt ggcgccgtag ctgatcgcgg ccgagtcgaa
      781 gatgtccctc tggccggaga gcaccaccgg gtagccttcg cggtcggtgc gggccaggtt
      841 catggcgatg gagtggttcg agagcgagtc ccagaaaatg ggcaccccgg cgcgggcggc
      901 gacggcgacg aagctcagct cgggatgggg ggcggtctcg gcgacccaga ggcccatttc
      961 gtagttgaac tcccagctgc tcagagcctt acccttgagg cggtcatagc gctcgacgat
     1021 aaaacggcgg atgagttcgt cctgagcttc gagggtctcc ttttcgcgga tgccgatatc
     1081 gtagatgcgg gtatccccgg cggcgcgcag ctcgtcgtca ttggccaggg gactgatggc
     1141 cttgaccggc aggccgaagg cgaagtggag gtcgtgatag acctgggcgc cggttgagca
     1201 gatgatgtcg atgaatccgg cttcgagcag agagatgacc atgccgccca taccgccagc
     1261 gatgccggcc ccggcgatgc cgagccagat ggtgtcgccg tcatggacca tgcgcttgaa
     1321 gaggttggcg ccgcgataga cgttgcgcgc ctcaaaggag gtggctccca gggcctcaac
     1381 gaggtcggag atggtctgat tgcgcttgac gcgggttgga atgatgtcgg gcgcgtcatg
     1441 aaagtaggag ggtcgttgga gatgcatagg ctcccttgca gtttagagat ggtgaggccg
     1501 gtggcagtga cgagagcgga aacgagaatg gcgcgcgccg gaggctgtcc ggtggaggcg
     1561 ccatcaatgg gtaacatcct gtgcggggca ctctattcca tgatcagaac agcagcggcc
     1621 agcacggtgg tccagaggcc gaccttgtgg ccttcagccg actggcagat gctgcggctg
     1681 acaaagttgt ttttctggtc gactttgtac tcctttttac gttcgtccca ggcgacctcg
     1741 gggtcgagct caatgccgag ggtggaggcg agcatggtgg cggcgaggtc ttcggcgaaa
     1801 tcggccgatt ttttcatggt ttcgccaaag ccatggtgct cgctcaggta accgtactga
     1861 ttggagtcct tgggagtggc cagaccgatg gcggccgaga ccaggcggtt cggctcgcag
     1921 gtggcctcgc gcgccatgac gacgaaggtg atctgcccgg gcgagagcag cttgatgccc
     1981 tgggcgcggc tgatgatctt gcagtcgggc gggaagatag aagagacggt gaccaggttg
     2041 cagatttcga tgtcggcatc acgcagggca agctcaaagc tctgcagctt gttcttgtga
     2101 tagccgacac ccttggtgaa aaacatgcgc ttcggaacca gaggatttgt tttattcaat
     2161 ttaggtttcc tccaaacgga ttgatgtgaa acggctaccg ccccagccga agacggctac
     2221 ggccataacg atgggagcat ttaacggctg cagcctagag ctccttcccc gctgtgaatc
     2281 cgatgcagcc gttccattta cccagagagt ttttaagctc atggttgagg gtaaaattgt
     2341 attgaaacca gggctgcaga gagagccccg acgtcagggt gcgggaaacc cgtccgtcca
     2401 gggagagcat gttaatggtt gcactctcga gcccgcagta gacggtgttg aacttttttg
     2461 atcccagccc gagtgtaaca gcagcgaagg ccgaccaggc ttcgctgaat tccttctcca
     2521 cctccagggc cggttccgca tagagggctc caggatactc gacgacatcg cagaagaggg
     2581 agagtttcgt cctgacgagg ccgaaggggc ggctgatgat cagggctgcc tcgccggtgt
     2641 ctggatcatc cggttgatca atgtatttat aatagttgaa atagacctca aaatccagat
     2701 tttcaaggct gaattcatgg ataagcgcgg cctcgatttc agaacgtttg atatcgttgg
     2761 ctggattgct gagggtccag ctgctccaga gatagaatgt caccccgttc agtgtcagcc
     2821 aggcggcggg ctgtaatatc gctcccttgt tgacggagag gccatgccat aggtagttgt
     2881 tggtcatggc catttccaca ccgccgcgcc aggagggctg ctcttcccct gtggaatcgg
     2941 catccgcggg ggaagccggg gtgccctccg ctgccgcgag caggaggagc acaatgagca
     3001 tggataccca tatgcaaggg tttgttctga tgcgattagc tttccaataa aacggataaa
     3061 aaatagaaat aattttagcc ctatgcaatc tatttttatc aggattttac cagcaagggt
     3121 ttgagatagt gcgctgtgaa ggaggctgag gtggccgcaa cctcctcagg ggtgcctcgg
     3181 gtgacgacat agccgccggc ctctccgcct tcggggccga ggtcgataat atagtcggcg
     3241 catttgatga catcgaggtt gtgttcgatg accagcaccg agtggcctgc ggcgatgagg
     3301 gcgttgaagc agtgcagcag ggtggcgacg tcgtcgaagt ggaggccggt ggtcggttcg
     3361 tcaaagatgt agaagacctg cttgcgtttg ggatcgccaa ggtgggccgc cagcttgacg
     3421 cgctgggcct cgcccccgga gagggttgtg gccggctggc cgagtttgag atagcccagg
     3481 ccgacctcct tgagcagctt cagccttttg gtcaccgcgt gttcgtggtc gaagaaatcg
     3541 agggcctcgt tgaccgtcat gttgagaacc tcggagatgt ttctgccgcg atactgaatg
     3601 tcgaggatat cgcggcggta gcgtttgccg ccgcagctgt cgcattcgag gagcagatcg
     3661 gcgaggaact gcatctccac ccgaaccacg ccggcgccct cacaggtttc gcagcgtccg
     3721 ccgggcacat tgaaggaaaa ggcccccgat ttcaggccgc ggacgcgggc gcgatgggtt
     3781 tcggcgaaga gctggcggat gggatcaaag gccttgacgt aggtggcggg gttggaacgc
     3841 ggggtgcggc cgatgggcga ctgatcgacc atcaccacct cgtcgatcag ctcctccccg
     3901 ctcaggctgc ggaagcgccc cacccgcttt ttccaccccc cctttttctt catcactccg
     3961 gcgtagagga catcatggac cagggtgctc ttgccggagc ccgagacccc ggtgatcacc
     4021 accatgcccc cgaggggaat atcgacatcg atgttcttga ggttgtgctc ccttgccccc
     4081 ctgatctgca gcccgggtgc acgcagcggg cgccgccggt ccggcagggg gatcatgcgg
     4141 ttgccgcgca gataggcccc ggtcagcgag cggccatcga tgcggatctc gtcgaaggag
     4201 ccgctgaaga tgacctcgcc gccattttcg cctgcgccgg gaccgaggtc gatgatctgg
     4261 tcggcctggc gcatcatctc gcgatcgtgt tcgaccacga ccacggtgtt gccgatgtcg
     4321 cgcagggcct tgaggatctc gaccagtttg gcgctgtcgc gcggatgcag cccgatggtg
     4381 ggctcgtcga ggatgtagag ggatccgacc agctgggctc caagggcggt ggcgagattg
     4441 atgcgctgga actctcctcc cgagagggtg ccggtccggc gatcgagggt cagatagccc
     4501 atgccgatgg tgtcgatgta gctgaggcgg ctgagaagct ccttgaggat ctggccggcg
     4561 atggccgact cgaacgggct cagctcgatg gttctgaaaa agtgcagcac ctcctgcagg
     4621 gatttgcggt tgaggtcggc gatggtcgct tcattgatgc ggaagttgag ggcctcgggc
     4681 cgcagccggg ctcccttgca ggcggcgcag gtgacgtaac cgcgatagcg gctgaggaaa
     4741 acgcgcaccc cgatcttgta ctttttactc tcgagccagc cgaaaaaagc ggcgatgccg
     4801 ggaaagtcat cgactccctt ccagatgatc tcccgggcct ccggactgag ctcggcatag
     4861 ggggcgtaaa tgtcgatgcc gtgacggggg gcgatgtgca tgagctgttc atggagttcg
     4921 cggtggctgg gggtgctcca tggggcgatg caccccgcgg cgatgctctt ttcggggttg
     4981 gggacgacca gctcctcatc gatggtgata atgtcgccaa agccattgca gaccgggcag
     5041 gcgccggtgg cgtggttgaa ggagaagagg ccgggctgcg gttcgggcag gacgatgccg
     5101 cagcgcgcgc attcataacc ctcggagaag ggctgcggcg ggccctcgga aaaatgaagg
     5161 tcgagatgcc ccttgcccat ctcgaaggcg aggtgggctg aatcggcgac gcggctgctt
     5221 atctccgcag cggtgatgag ccggtcgacg atgacgtcga tgcgctcccc ctccgggggg
     5281 gcctcatcga aggagagggt ccggcccccg tcaaggtagc ggtggtagcc cttcaccctc
     5341 aggctctcca gactggcggc gtcggcggga tgggggaagg tgatgatgca gcgggtgccc
     5401 gacggcagcc cgtgcaaatg ctcgacgacg tcgctgacct cttcccgccg cacaggctcc
     5461 ccgcaggagg ggcagaaggc gcggccgatg cgggcgtaga gcagacgcag atagtcatgg
     5521 atttcggtgg cagtgccgac ggttgaacgg gagctctggc tggtgttctt ttgttcgatg
     5581 gcgacggcgg gcgaaatccc gtagatctgg tcgacatccg ggcgttccat cctctccagg
     5641 aactggcggg cataggccga gagcgactcg acatagcggc gctgcccctc ggcatagagg
     5701 gtgtcgaagg ccaggctcga tttacccgag cccgaaaccc cggtgatcac caccagctga
     5761 ttgcggggaa tggtcacgga aatatttttc agattatggg tgcgcgcccc gataatctcg
     5821 atggcgttgg acatggattc ccttaacccc gggcggataa caattggctg acctgaaaaa
     5881 tacgaaaatt aaaccaaaca ttcaataaaa tagcacgcgc ccccagcgct gctggccgtc
     5941 ttttgtctgg ggatgataat cggcgccagg gcaggctgct gccggcagtg cggtttcgct
     6001 gcgcatggtg tggagaaggg tgttcgcccg gggcgggctc aggccggcgc ggtgagaatg
     6061 cgccggcgga cgagcagggc gtggaagagc agggcgagcg cgccgccgat gagggcggtg
     6121 atgagctggg gaaaggtgag catggcgatg accggctcgg gcagggtccg ggcgaggagc
     6181 agaggcatga tcaggcgcgc cgccgcgaag agccaaagga acttggcaat cgagccgagc
     6241 agcaccccga gccatgcccg ccacgagcgc aggggcaggc cgcagccctt tttacagaag
     6301 aggcccagaa aaaggacgta gagggcgttg ccggccagga tgaaggggac gaagggggcg
     6361 agcagggggg gaagctggcc gcgcatgagc gcaatcaggg gggtgaggca gccgagggcg
     6421 atgccgccgg tcgtccccgt gatcagggtg gtgagggcga gcatgagatt gaccagcggt
     6481 ccggtgaaag gctggggcag tccaatcatc tgaacgatca gggcgatggc gatgagcagc
     6541 accatgcggg tgagcctggc ggtttttccg tgcatgtggg gcgtctattc cgtgcggtta
     6601 aagaccaggg gcagattgac ggtgaccgaa ccttctgtga ttttttcaaa acggaggcgg
     6661 cgcagcaggc cgagcagttc acggacgaac tcctcgctgc cgatgttgct ttcgacgacg
     6721 gcgatattgg agacagcgcc gtccgcctcg atggtcaggt cgatgttgag cttgccgcgc
     6781 atatccggat tgatgcgcag gtgtttgtta taaatgtaca tcatgcgggc ctgctggcca
     6841 ttgatgaccc ccatgaccga ttccgccgaa cgctggacgc gggcggtttc cgagccgcgg
     6901 atctcgcccg gggcctggat gttgacctgg ccgcgttttt ccagcttgac ggtttcgacc
     6961 cccttggctt cggtgatgag ctggtcgaca ttgccgagcg cggcgacgtt gaactcatcg
     7021 aggacgacct gatcttcggc ctggtcggcc gagctgcggc cgcggccgga tccccggccc
     7081 gatcccagac ccaggccccc ttcgccatat gttccgccgc cgctgccggt gcgcagtttg
     7141 gcctgggaga gcaggcggtc aaggtcctgg atcgaattgt cgctgaccag aaattcaatg
     7201 ccgttgtttt tcttgtcctt gtcgctgacc ggcacaatca caccgagcac gccgatgatc
     7261 cccgaggtgc tgccaccgag caggttatcg ccaccggcgg gctggttgag gtcatcagcg
     7321 atggaagggg gcggctcgag ctcgatatat ttggtgatgc gcactttttt ctcgatcaac
     7381 tcggccggct gcaggtagac ggtgagccag tagatgccga ccccgcaaaa ggcgatgatg
     7441 ctggcgatgc cgagggccca ggacatgtgt ttctggcggt agcgcttgag ttcaggggcg
     7501 ccgtagctcc tggagatctg ctggggagtc ggcttcggcg ccgggggcag gtcgtaaacc
     7561 accctgcgtt ccttctcctc caggctggcg agctggtcga tctccgtttt gaggcgggcc
     7621 atgccctcgc cgagtggacg ccgctggctg acgatgacct cgacctcgct gcgagtcacc
     7681 gagtcgaagg ggtggctcag gcactgctgg cggacggccg ggtcctgcag ctccgcccgt
     7741 ttccggatca aaccgtggtc gcgatagagg gtgaagagac gttccagcag ggcactctgg
     7801 agtccggcca actgtgcgcg ggtctgttcc agctgccgcc gctgtttttt ataatcaggc
     7861 tgcattactt cctcatagtt gccttaccag gggaaaacat ccttgcgttt gctagtggat
     7921 gacggttttt gtctcaggcc gatgcgtccg gcgggttcca ccacgcggaa gatctcctcg
     7981 aagaaggagc gctggatctg gatatcgtca atctcgggat cgctgccggg gatgatgaag
     8041 atggcctggg gtttttccag ttttccctcc acctcgatct tgtcgagaaa gagcttgaag
     8101 gattcctcat cctcccgcag cgagtccggc agggaggtgc gcggcgccag cagccgcagg
     8161 gaatcctgcg caccggttga agaggcctcc gtgctgaggt cgttgaacag cgctgtgccg
     8221 tcccggtccg gattctgctc gccggtgcgc ggggccgaga gcaccgcggt ggtgtcggtg
     8281 atatcgaacg gccgctcttg cgcgtcagcg gtcccgaagg aggccgccag cagcagcgcg
     8341 gggagcagca ggactatggg cagcgtggta cggcggctca tgaaggggtg gctccttcct
     8401 ggttagccgg cgtcgtctcc tctgtgtttg cgggcggagg cggctccgca gccggagcga
     8461 cccctagttc gatctgcaag gcctgcaggc gctcgcggct ccggctcacc cagttgtttt
     8521 cgatggcatt ggcccgggcc tgttcgacca tcttggcata ggtttccagg gcgcgctcct
     8581 tgtagggttt ggccgcctca accagcttgt cctgatactg ctggagggcc tcccccttca
     8641 atccccgcgg cgggggcgag gtcatgaagg cgcggacgaa ttcctcgaag gaggcgccga
     8701 tgcggtagga ggcggcggtg gaccagtcgg cgatctggaa ttcgagagtc tccttgtagg
     8761 cctcaaagac ctcgttgaac ttggccacct tgcgtttcat gctgcgctcg aaggggggct
     8821 gaagagagat ctgttcatat tcgctgaaga gcatctcgcc gagcatgaac tgggcctggg
     8881 ccacataata ggggtcgata tcggcggcgg cctggccgtt ttcccgccag ctcgccacgg
     8941 ctctggtata atagcggcgc gcgccggcgg agtcgccggt tttctgggcc atctcaccgg
     9001 ccttggccag gctctcgacc aggtcggtga gggaatccgg ataggtgagg gcgaagcggc
     9061 tgtagagcct ttccgcctcc gcccagttct gcaggttttc ctggcagatg gccgccgatt
     9121 tcaggctgcg ctgggcgtaa ttcgactccg gccaggtatc acagaggagg aggtaatcat
     9181 gcacggccag ctgccattca ccctcggtct cgcgcagtac ggcggcgcgg tagagggcct
     9241 cgtcggcgag ttcggagggg ggctgctggg cggcgagctg ttccagggcc tgggcggcct
     9301 cgcggttgtt cttgagtttc tgaaattggg cggccgcttc gaagagggcg cggtcgcgca
     9361 gagcagtttc gttcgcggcc atggccacac tctgcatgag gcgggcggat tcctcgcttc
     9421 gtccccctgc ggcgagtttc tcggcgattt taaacctggc gagagtgccg aggcggccag
     9481 cccggtcgcg ataatgggcc gagtcgggga actgctcctg cagggaggtg aaccagcggt
     9541 cggcctcctc gaaacgattg tcgtcgaaat agcactggcc ggcgttgagg gctgcgctca
     9601 gatagtaggg acggtcgggc ccgagatcga ccacatgttt gtagacccgc gcggccgcag
     9661 cataatggtg catctcgtgg agggtctcgc caagcttcat caggacctga tcgtggtttt
     9721 tgctttccgg gaagaggcgg atgaactcgt tggctgccag gaggaggccg ctttcggagg
     9781 ggcgcgagac ggccaggcgc agggtgtcgc cgctgccgat aaagtcgggg atggccacag
     9841 ccgcggaatc cccgccctgg tcgctgccga gaagctgata atggcagagg acgcggttgt
     9901 aggcggcgtc atggcggtag cgggtggtat cgtattgggt cagtactgcg gtgtaggccg
     9961 tgatcgcagc ggcatagtcc gcccggccat agttgcattc agccagaaaa tagtgaatct
    10021 ccgcagcgtc cgcggccgct ccatatttct cgagatagtc ctcatatttt tcaatcgcga
    10081 tctgataatc ccgcacccgg ccgttacgct gcgcctccgc ctggtgatgg atgcccagac
    10141 cgcgcagggt attacgggcg aggctgtcgg ccttgacatg gacgggaccc gaggcgtgct
    10201 gaagcagcca aaagcccccg ggggcgaact gctgcacgat gcgttcgcgc gtcagtaggg
    10261 cctgggcagg gcggctgtcc gcctcataat tgtcgaccat ctgctgcagg acctcgggcg
    10321 cattctcgta gaagggatag agggtgagca gaacctcctg ggcggcgatg gcctcgggat
    10381 agtaggcgcg cttctggtag agcgcggcca gctggacgag gatgggcagg gtgtaggatt
    10441 tgtcggtgcg gctggcgaaa aagctctgcg ccgcagccgg accgccgtat tcggcataac
    10501 agctggcgat atactggatg gcctccgagc tcaggtccgc ccgggagcgg ttgagctgct
    10561 gagaatcgct cctttcgatc agcgccatgt cctcgagcag atagataaag ctggtgatgg
    10621 catggggata gtcgccgagg gagtagtagg accagccgag cttgtagagg gccatgtcaa
    10681 aataggggtt gtcccatttc tcgagcagag gtttgtagtg ctcgatggcc ggttggtatt
    10741 cgcggcggtc gaaatagaat tcagcgatgc ggaaatggga ctccagggtg atggcggagg
    10801 tgggaaattc actgatgatc cgctccaggc agagctgcgc ccgcacgcgg ttgccctgct
    10861 gcagataggc catgccaagc atgtagaggg ccttgtcgcg aaaactcacc ccgggatatt
    10921 tttccaccag ctgctcgagc aggctgatgc ttttttccat gttggtgcgg ggaatttcgg
    10981 gctcgtcgag aatctcgttt ttctcaaagg cctcaagctg cttctcgtac tgctccattt
    11041 gctgctgaaa gagggccgca gagtgacgct ggtagagctg ggctagctgg aggagtacgg
    11101 tgggggtgaa gtcattgtcc ggatatttct gcagcagctc ttcatgctct aggatgatgc
    11161 ggttgagctt ttccagatcc tcgccgctcg cctggaccac cttttcgaca atctgaccgt
    11221 tgttccggat ctggcttccg atggccggcc ctgtgaccgg cagaaaaaga aagcaggcca
    11281 gcagcaaaag aggctgcagg ggcaaacgcc ggccatgcgg ggcggaggct gctgtcttcc
    11341 gtattggtat tactgcttta tttctcatag gatgtgccca tctggtctcc gccctactgc
    11401 atgctctgca taaagagggc gcgggatttg ccgaactcgg cataggcgcg ccagtccatg
    11461 cgcgattgtt tccgccgggt catccggcgc agctcttcca ttccgtcggc gagttggttg
    11521 acgcggtcgc gctcctcctg gatccgccgc agcaggccct gacggagatc gaggagctgc
    11581 tgccgctgtt cacgcagctc gtccggcatc tccggccagg tgacctggag ggggagggcg
    11641 tcgatgagct cgctctccat gatgagcagg tcggtctgga gtttttccac ctcgatgccc
    11701 tcatccagaa gcgtcatttg cacttcccgg gagatgaccg gcatctcctc cctggagggg
    11761 aacatggaga tgagatggtc gtagacgcgg atggcgtgat cgtaacgctg cagcttcatg
    11821 aagcagcggc cgagaaggaa gagaccctcc tcgttgctgg ccgagccggg ataatggatg
    11881 aggagggtgg tcagcggttt gatcgcggtc gagaggcgac ccatctggac ctggcaccag
    11941 ccggcggcca gcagggcctc gtcgcgatgg agatgggtcg gcgagacgag ctggaactgg
    12001 tcgtgggcct gctggtagcg ctcgagttca taaaagaggt agcccagggt catgcgggtg
    12061 tcgtcaatga gggtgcggat ctgctcgctc tcgaccggca ggtcgcagat ggctcggagg
    12121 gtcatgacgg cgtccgggac ctgcttcatc cgcatctgcg ccagggcgag gttgtactgg
    12181 gcgaaggggt accaggggct cttgatcgag acccggacca ggctctgttt cgagttggga
    12241 aagtcgcgga gctggtaata ggccaggccg gagaagtagt tggaggcatc ggtgaccgcc
    12301 tggggcgagg cggagcggat gatgatctgg tggtattcga tgcagcggtt ccactctttg
    12361 cggtcgtact cgatgcgctc aagtcccagc aaggcgttgg gctggagtgg gctgcgcaga
    12421 tactgggtga cgaggttctt gtagagccgc tccgccccct ggggcaggcc gatctgatag
    12481 aggcattcgg ccagggaaaa gaccacctcg tcgctttttt caaagccggg attgaagtcg
    12541 agcaggatga tcagatcgcg ggccgcttcc caatagttct ccgagaggta atggcgccgg
    12601 gcctggctga gcagccgttc cgatgatttc cgccattcgg aggtggcctg tgccgcggcc
    12661 ggggcgggca gggaagtggt cgccatcagc gccagcagca ggacggtcaa ggccaggtgc
    12721 acgcgccggg ccggagccgg ccgggaggcg gctgtccggc ttgcgggggc aatatgtcgg
    12781 cggtcgcgcg gattcatggt catagtccta ttgcggcggc tgccccgggc tgttttcgat
    12841 ctttttgcgc agctcctcca gttcacgatt gacctgttcg cgctgtttgc ggatctcttc
    12901 gttctttttc tcctcctctg cgcggcgttc ggagcggacg cggtcttgct cggtggcctg
    12961 ctcgaagagc tggatgcggt gggtgacccc gaagagcacc ttccagtccg cataccgttt
    13021 cagaaaggga ttggctgcca tctcggccgc gctcggggtg taattgccga ggcggacgtc
    13081 ggcggcgacg tcaaagacca gctgtgcggg cccgagaaag cgcaggccgc tggagaaacg
    13141 gagcaggttc tggcgcaggg aaaggctttc accctggttg ataaagatct ccccgctggt
    13201 ttccacataa agcagtgtgg cccggatcgg gaacttgaag ccgagggcga cgaggagctg
    13261 gtcgtaatca gcggagaagt agcggtcgct ccagtcctga tcctgatagc cgatattggc
    13321 gaggaatttg accggcaggg cgagggagct cgagcgcagg tcgagcgaag ccaggccggt
    13381 gaggccccag ctgtaggcgt cggcgctgta cccctcgtag gggatgttgt gcatcggcgc
    13441 ggtcggcagt ctgacgtagc cgaggaggcc ggtctggaag atgctgcccc ggttggggag
    13501 atggaacttg acgccgatcc gggtgtcgcc gatcgggccg aagagatcgc gctggttgtc
    13561 ctggtagggc acggcgtgga gcatgagctc gaagctgcgg gagagcccca gggttaaggc
    13621 gatattgagg gtgtggtcga cggcgagctc ctccctctcg ccgatgcccg cgccgaccgg
    13681 aaccggcgtg gtgatttttt cgagatagag gctgtagagc ccgttgatgt agagctgtcc
    13741 cgtcggcact gggtcggcat cgagggcacg cagctggcct ttgccgccgc tcagtgtgac
    13801 ctgcgtcagt cccgcagtag gggcgagcag gatgaccgga atgatcgcaa ggaggagtat
    13861 gcttttcagg ggctgcatgt gcatgattta ccgcttttgg caaaaaaaca gccggttcag
    13921 ttggtgcaat gacctgttct attctcggga gcatcccgga tgccgtacac ttgtgccggt
    13981 actgttccca ttacagtagc cgcaatatgc cgttgatgca gaaactgtgc cgctacagca
    14041 tccccttgcg gcggcggaga aaccactcca gggcgagcag cagcaggatg gccgcgagca
    14101 gggccccgcg gttccagagt tccacatcat tggtcagggt gcggtgaagg ctggcgccct
    14161 gaatagcggc gatgagtgcg gccgccgaat ccgacggggc atagctccca ccgctgagct
    14221 gtcccaaccg gcgcagaagg ctctccttga gggcgacgtc gctcaactcc tcgttatagc
    14281 tgccgacgct gaagaggtgg gtggcctctc cgaggagcgc cccattgcgc cggatgcgcg
    14341 catggacccg ataatccccc gccttctccg gacgaaaggc gccgctgtag ctgcccttgc
    14401 cggtcagggt aaggtagagg gtgttctgca catcgccctt gtcgccactg gtgatgccga
    14461 gttcaacctc cgcgtcatcg accggctgga agcgggcatc cacgatctgg gcaacgagac
    14521 ggacctcgtc gccaaaatga taggtggact gatccgtgcg cacccggatc aggtcgctgc
    14581 tctgctcaat ctggagccag cggatcacat tctggaaaaa gtggcgataa gtctcatcgg
    14641 tattcccgat gccgctcatc atcaggtgcc agcgccacag ttcgtagccc ataacggccg
    14701 ccgattttgc gccggcgctg cgcacgagaa tgaaaggcca ttcctccgca ccgcttccgg
    14761 gtggtgccag tccggccggt cgggcgtggg cgaggatttc ggcatcgggc caccagccgc
    14821 gcagctgctc ccggcagtag accggcggca gcagattcca ggcggctgtg ccggagccgg
    14881 ggatctgcat gaccggatgc tgctgccccg ccgcggtcag cagcggagtg acctccatct
    14941 cgcccgttgt gaccacgctg gcccggatgg gcaggaaagg ttcgagcggc ttgacacggc
    15001 tccagtcggt atcgcggccg ctgatgaaga ggagcggccg cggccgcctg gcgagcgcgg
    15061 cctgtaatag agagagcgtc cgcggggagg aggtacgccg cggaaagttg tagagcacca
    15121 gcagatcgct ctctccgatg ctgtcctgca gcgcccgggt ccggtcgagc tgcagatctc
    15181 cgttgtcgcc gagctccgcg agcagcttca ggtcgaagcg ttcgtctgcg gccaggtggc
    15241 tgcgcagcag cgagaggtcg ggcgaaaggc tcccggccag tacaaagatc agggttttcg
    15301 atctgagcac gtcctgatag agcgtgaacc ggttgttggc acggttggcg tccatgcccg
    15361 cgggctcgag gagcagctgg agcttttgcc ggccaggagc ggccggtgta taattgagaa
    15421 caatcgccga atccaggggg ccgcgctgga gatcgatccg ggcggcgctc tccctgccgg
    15481 aggccccctc aaggctgagg ctgatgtttt cgcgccggct gctcgtgctg cgcacggtga
    15541 cgctgatcgg agttgactcc ccggcgaagg cgaagggatt ggcctcgaca tgggtgatgc
    15601 cgatgtcggc ggggacctcg gcggagccga gtccgatggc atggatcggc accccgattt
    15661 ccgcggcata tcgtccgggg tctccgccct gggtgtaatt gccgtcgctg agcaggagga
    15721 tcccggcgag attctgctcg accaggctgc tccggatttt ctcgagggcc ccggtgatgt
    15781 ctgtggcatc cccgttgaag gtgagggaat cgagcgcatc cggagagagc tcgcgggcat
    15841 gggcggcgaa gccgaaatag cgggtggcca tgccgcggtc gagctgggcg ggaagggcct
    15901 cgcgaagcag gcgcatcgtc ctctcgctgc ggctgattcc cccttcgctc tgctgcatgc
    15961 tcgccgacag atcgacggca acggcgagca gggggggcgt ggtctgcacc cgccggaact
    16021 gcacagtcca ttcaaagagc gcaaggatga tcagcagcag ggccaggctg cgcaaggcgg
    16081 tcaggacgat gcgcacggcc ggctgcacga ccggattggt ccggcgataa acaaagaggg
    16141 cgatcgcgac cgccgccagg gcggcgaggg cgagataaag ggggctgcct gcgatcatgg
    16201 cccggaaaga gtcaggagaa aaggacatgg ccggaactca tccgcaattt atacaatata
    16261 ctactcttta acataaaatg cccacggatg ttccacctcc ctgtgatgcg aagcacagta
    16321 aatccagaaa atagccgtca tagatcgccc cggatttgtt cgactggggg ctaaagggag
    16381 aggttgggtt ccacatcaag ggaaaaatcg gagcgctcgc cagccaggag gcgctggcgt
    16441 ccggcctggg cgatcattgc ggcgttgtcc gtgcacagct cgggcgccgg gatggccagc
    16501 gcgatgccca gttcgccgca gcgggcggcg aagatcttgc ggagcgggcc gttgcgcatc
//
LOCUS       DAOPWU010000032        16458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3580_length_16458_cov_4.353838, whole genome shotgun sequence.
ACCESSION   DAOPWU010000032 DAOPWU010000000
VERSION     DAOPWU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16458
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3580_length_16458_cov_4.353838"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKM23_03295"
     CDS             <1..788
                     /locus_tag="PKM23_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNY90530.1"
                     /translation="DLEQQYAALHEEKQAAIGGLLTAMRRGDAAGEACAGSGLMQTNS
                     QMQAVRESAIDLLADRHPGMDRKDTNYVFLTFLVHYLPVGLLGLVIAAIVAAAMGSTA
                     AELNALAATTTVDFYRRFRRRQPRAAAAGSAKGGGNDSAGAQASAAEERHTVRFSRAA
                     TLFWGLFAVLFADYASRLGSLIEAVNILGSLFYGTILGIFLTAFYMPRVNGRAVFRAA
                     LLAELLVLACFLWSDISFLWYNLVGCLAVMGLGWLQSVNLHDE"
     gene            874..5076
                     /locus_tag="PKM23_03300"
     CDS             874..5076
                     /locus_tag="PKM23_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNY90531.1"
                     /translation="MKGLSKYLLAGMMLIFTGVLIASPVEQVGVILQPMGLLKAVESD
                     SDLVPVEFEFKFLKFATDSGVGINGTFNNWGDVFPMRLAIPLDGWTLTMKLAPGSYVY
                     KFVTYTDTVGQAGVTGWYPDPLNPITQGPFRDSFIAVSDPMIYYPSPMPDADITDSTP
                     LLSFKIAHSPRRTLDRDACELIIDGIPVANAGRYYSTVTHRLSYTPKEPLFKGAHTAT
                     LRIRTTDGATAEYTTSFNTLSGVGDTPVTLSFDGRSPHLVLNGVPAEATLEWMDLPLS
                     HPLTDPDGDGFFTREIRMKLNDPHYYRVVVDKQIYLEFDPGNPLLNEEYRSVVVNHFE
                     LNGAFRFLSPRPDTLFDGRLQAFTIRAAAVPGDSGFVLDESSLQVEMDGAAVPWSLNT
                     GRDPVEIEITAPLTPGRHFVKLAAGDQGGEPFTPGYLAFGVYPAGSGLHVVDAERDDT
                     GPGSYRYPAGVTEGCADIRSAHVAVNAGLDSLLFEIEMRAVADETRLGFLLYSRLGKK
                     LTDAPEALELQIPAWQGTGLYATLAAPGGQFFRPDRDNLLLAEGAAPAIALSLNGSAQ
                     EENRLRFAVALADLEKLIGSFSGEWHVTLYSFLLNAAGSIELDGASGGVSAADEPDVY
                     DVAFCSNDEVQAQLLANYIYAYKLGGPRTAAIGTDLRGSWPLQPAALHPQLGKGTLLR
                     LRTNGGDIFRREVRLAGEVSDPAVTQVQVQVNGAAVPAAIQGGRFTADITLQYGVNEI
                     SATAAGTGGHELHSQSVRFTCRLDTLPDVRITTSVTEQLVTLDGSATSDPLGRELTYS
                     WSADPRNPQAVAWSGGDAALVTFAAPAAPGEYYFTLTATPAGGAAAWARTVVLVEGGQ
                     PRTADLENWHPAWIDSAVAYEIYTRAFSRTGRLANITSKLADLRDLGVTVLYLRPIHP
                     STAIHGYWITDYYGVNPEYGTHADLGNLVRTAHKLGLRVVLDYVVNHTVDTHPYMQDA
                     LAWGEDSPFYDFYRWNQDGNFHYYFTWVNLPSINFESPHTRQYLIDMACWWVQNFDID
                     GFRCDVAHRIEKDRPSGPLFWQEWRRALKALKPDLWLLAEADADDAAYYDKKFDSAYD
                     YRWVNTVINLSNNLASAEQLHEVVQYYDRPEFPAHALPKRSLEDFDNSRFLANTTVEQ
                     SMLAAALQLTAPGVPMLYAGQEVGEVSDRGLVSWSDPRQLKKYYRSLVQLRRKNPALS
                     VGDYTPVPTDQPLVYAMMRADSFLVVANFGSSELTARLSVPAGLIPGDSLKVFYLNDQ
                     LVKLNLPVTAAALRAYDLWLPARTVRIFKLGAAATGIEKAEERPEVFGLTPNFPNPFN
                     SSTEIRFAIGGAAPVTAELDIYNVLGQRVRMLVREKRGPGRYSVLWDGRDDRGQALGS
                     GIYFCSLKAGDFAQSRRMLMLR"
     gene            complement(5170..6852)
                     /gene="ettA"
                     /locus_tag="PKM23_03305"
     CDS             complement(5170..6852)
                     /gene="ettA"
                     /locus_tag="PKM23_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNY90532.1"
                     /translation="MANEPNKVIYSMIGVSKIYQQKAVLKDIYLSYFYGAKIGVLGLN
                     GSGKSTLLRILAGTDKDFVGETVLSPGYTVGMLEQEPLLDETKTVREIVQQGVQEVVD
                     LLAEYDVINGRFAEPMSDDEMNKLIERQGQVQEQLDQLNAWDLDSRLELAMDALNCPP
                     GETPVRVLSGGERRRVALCRLLLQRPDILLLDEPTNHLDAETVLWLEAELNRYAGTVI
                     AVTHDRYFLDNVAGWILELDQGEGIPWKGNYSSWLEQKQARLLLEQKKETERQKTLQR
                     ELEWIRMTPRARQAKSKARISAYEQLLQEGSEERRKELELYIPPGPRLGKIVIQAEQI
                     SKSYGDRILFEELTFSLPPGGIVGVIGPNGAGKTTLFRMVVGDEQPDSGVIRIGETVQ
                     LAYADQSRAALDPAKTVWEMISGGEERIKLGDREMNSRAYVAQFNFIGADQQKKVGQL
                     SGGERNRVHLALALKQCANVILLDEPTNDLDVNTMRALEEGLLNFAGCAVVISHDRWF
                     LDRICTHIISFEGDSGVVWYEGNFSDYQENRRQRLGKAADVPHRVKYKRLTR"
     gene            complement(7036..7446)
                     /locus_tag="PKM23_03310"
     CDS             complement(7036..7446)
                     /locus_tag="PKM23_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90533.1"
                     /translation="MKKGMMTLVLAGLLAGFSYAADSTTTLDSARLAVAVDNYKMALK
                     SDNPGVRSSALYMLALIRSRYPEANISGFNGDLARISRKDADPLVRVQANLILTYLNS
                     DSLHTKIRIADPEAPLAFYSELFTEITMNPEEIK"
     gene            complement(7844..>8200)
                     /locus_tag="PKM23_03315"
     CDS             complement(7844..>8200)
                     /locus_tag="PKM23_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY90534.1"
                     /translation="PRELYTLLANYPFPGNIRELEGMIFDAVSRHKGGVLSLTAFRRA
                     IEPGWDETEQPAEHGGEEAGEESISFGKTLPTLRAAEQALIAEALRRAEQNQGIAAGM
                     LGLTRRALNNRLQRNK"
     assembly_gap    8201..8210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8211..9273)
                     /locus_tag="PKM23_03320"
     CDS             complement(<8211..9273)
                     /locus_tag="PKM23_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY90535.1"
                     /translation="MNSALYPEWPVLLIDDEENFLRSASYTLISEGINNVRSCQDSRQ
                     AMGMLAEESFSVVVLDMMMPHLTGEELLKLIVRDFPDIPVIIITAINEVEWVVKTIKA
                     GAFDYAVKPVDDARLVTLVTRALQLREVRDENIKLRQYLISARLEHPERFAAIITDSP
                     AMRGIFQYVEAIAATPLPVLITGETGTGKELIARALHDLSGRKGDFVALNVAGVDDHF
                     FSDTLFGHSRGAFTGADRERKGLIEQAAGGTLFLDEIGDLSLESQVKLLRLLQEGEYY
                     PLGADQPRTSDARVTVATLRDSDYLQKSERFRKDLYYRLKSHHIALPPLRQRREDIPL
                     LVDHFLDKAAAKLDRRRITP"
     gene            complement(9298..10545)
                     /locus_tag="PKM23_03325"
     CDS             complement(9298..10545)
                     /locus_tag="PKM23_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY90536.1"
                     /translation="MNNTNASGKTAAGGRRRPRDAESALSRDEQRVRRLLQAAVDYIY
                     TVRVENGRAVETLHGPGCVLVTGYTPEEYRDDPTLWIRMVPEEDRPAVQAQAEAVLRG
                     EATLPVEHRIIHADTTLRWVKSVVVAHRNDREEVTEYDGLINDITARKQAEELTIRQQ
                     EQLIQADKMATLGTLVSGVAHEINNPNNFILLNAKLIAKAWKDLLPAMHHYFLHEGDF
                     RIAGMPYSRAITGIPELTQAIVDGTVRIQKIVQSLKDFARSDPGELNQNFTVNAVVEA
                     AMMIVANLLRKSTDHFAVELGDSIPAVRGNFQQLEQVVINLLTNACQALTSRDKSITL
                     RTRHDRARHRAVIEVADEGKGIDPEQARYIFDPFFTTRRDIGGTGLGLAISYNIVKHH
                     GGELLFTSRPGEGTTVRVILPLP"
     gene            10730..11533
                     /locus_tag="PKM23_03330"
     CDS             10730..11533
                     /locus_tag="PKM23_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90537.1"
                     /translation="MGHNHHTAVTGKGHSRKPCLLLPLAGLVLLSFLFARSAAAQPRH
                     ERLIYSHPLTSAADDQGLLLRSTDGRFGASGWQSLSPGSQLLITLPEGLPLEGTCSVR
                     VSNFDPCEQNVSEKQPIINLYSQPCGNKEIYETDGGWFHLISGTLYQTGDETAGFKFW
                     AAPRGVESKTEEEVMLEARWNPARSYEFTFIWDRSTLFFLVDGVERMRLAYAGQVEPF
                     RYIFLGKDNLTRGYTAQPGPVFSDLRLYAPAEQDPPPAPPAGVRVIHAE"
     gene            complement(11561..13342)
                     /locus_tag="PKM23_03335"
     CDS             complement(11561..13342)
                     /locus_tag="PKM23_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90538.1"
                     /translation="MRNLIGILVLSGMFSAAAQSNPVLLVSPERPAAGGSFRVLAAWE
                     ADPGKSQIVVTGPGGPLDVRTGREGGGPPWWRCAGFEAGAAGRYQVALKSGRTILASC
                     EVEITPGPAPVRKPAAGVWENRRAWGRESELLYAAWIEALFAGAPEGSSWPSLHEVTR
                     RAEENPLFNHLGLGEDDAAGKVSVIMEPDCADNPFYLRAYFSWKLGLPFGFHETGRGT
                     LKQPPHTGRWITNTSHPGQPHPVRAFNAFLREVMNTIHSGAARTRLEDESSDYYPLPL
                     TRAALRPGAVFADPYGHTFTLVRWVPQSGRKPGLLLAVDAQPDGTVQIKRFWKGNFLF
                     TTTGVIGEPGFKAFRPIRVEGGKPRLLSNREIAAAPGYGAHSLEQKGMSSEHFYDTME
                     RLINPEGLDPEAALLELIRALQEQLIVRVESVANGEKWMQAHPGAVIPMPGSAAGVFL
                     AGGLWEDYSTPNRDLRLLIAIDAVLDFPARIVRAPGNYKISRWKNPQKIRAELEELTR
                     EKSGEMTITYTRGDGSKQQLTLAEILRRAQAFEMGYNPNDGVEIRWGAPAGSEEIKSC
                     RRRAPAGQLEQMRKLRPWFQKRLHPPT"
     gene            complement(13351..15618)
                     /locus_tag="PKM23_03340"
     CDS             complement(13351..15618)
                     /locus_tag="PKM23_03340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNY90539.1"
                     /translation="MNLIRIALSIALATATVIPTAAAEPAGAATAAAKAAPLAGSRLV
                     PADHPWIQYFGRWDFTDPLAPAHGWPGVYIRADFEGTSLGLRMTDDAYYWNLFIDGEP
                     VTIFHGSQSGTTSYSVVEGLPDGQHSLLLTKRNEYWWSRFTFNGLLLDEGRDLLPPPE
                     KPARRIEFIGDSFTSASGNEYAYTDTPPDPAPYTNIFEGFCPITARHFNAQYHMCSIS
                     GYGMAVDYKGDATGTIPALFDRAVVYNSAIKWDFSAWQPDLVVICLGLNDYSGLGGWS
                     GGVSAGNQTLFKNRYHEFVARLTGLYPAATILAFAPHVELLQELTAAVVREAQAAGLG
                     RIHYADFPYFDTGYVNGGHPNVATHHQIAGRLIAAIDSLRIWPEEADVTPPRFTALPA
                     DEPFTLYSPLLELTVETDSYASVRWSEEDRPWAQMEHTFATTGRRLHRTTIPVEHGRS
                     YTLYLHAIDDAGNATASSAVLRFTVDTTRAVRRWQELEYDDSAWKTGPAPLGSSAAAS
                     PRTLIAPVDAAYFRRTFTIEDLSAIAGMGALIKCSDGAIVWLNGEEIQRINMPAGAPG
                     YSLFASKSAAMSSMVVLNLQNGLLGKLRSGENILAVEVHRSSASPWLAFDSQLTDNLN
                     RFYYKLGSEWRCWDGGAAPEEKVQEKTSRVQPGVQPPGIFLLEQNYPNPFNPSTLVRY
                     HLAAPAQVRLEVFDLAGRRVALLAEGLRQAGAHEARFAPGEAGLPGGCYFCRLLISPG
                     DGPGRTLTRKMLYLK"
     gene            complement(15635..16159)
                     /locus_tag="PKM23_03345"
     CDS             complement(15635..16159)
                     /locus_tag="PKM23_03345"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNY90540.1"
                     /translation="MLKQLNPSELVISPFDILDKSWALLVAGVEHPNPMTVSWGGFGT
                     LWNKPVVTLYVRPTRYTWQLLEEHPEFTLNVMPARFHGALNYCGSHSGRHINKWEKAG
                     LTPQESTTIAVPRVAGAALSFECRTLAFQDFDPGRFLQPGVEGNYPQKDYHRIYWGET
                     LAIWIDDAFTPGQE"
BASE COUNT         2863 a         5168 c         5264 g         3153 t
ORIGIN      
        1 cagatctcga gcagcagtat gccgcgctgc atgaagaaaa gcaggcggcg atcggcgggc
       61 tgctgacggc gatgcgccgc ggggatgccg ccggggaggc gtgcgccggc tccgggctga
      121 tgcaaaccaa cagccagatg caggcagtgc gcgaatcggc catcgatctt ctcgccgacc
      181 ggcatcccgg catggaccgc aaggacacca actatgtctt tctcaccttt ctcgtccatt
      241 acctgccggt tgggctgctc gggttggtga tcgccgccat cgtcgccgcg gcgatgggct
      301 cgaccgccgc ggagctcaat gccctggccg cgaccacgac ggtcgatttc taccggcgct
      361 tcagacgccg gcagccgcga gccgcagcgg ccggcagcgc aaaaggcggc gggaacgact
      421 ccgccggcgc tcaggcctct gcagcggagg agcgccacac cgtccgcttc tcgcgcgcgg
      481 cgacgctctt ctgggggctc ttcgccgtcc tcttcgccga ctatgccagc cgcctgggct
      541 cgctgatcga agcggtcaac attctcggct cgctctttta cggcaccatc ctcggcatct
      601 tcctgaccgc tttctatatg ccccgtgtca acggccgtgc ggtctttcgc gccgccctcc
      661 tcgccgaact attggtcctc gcctgcttcc tgtggagcga tatctccttt ctctggtata
      721 atctggtcgg ctgcctggct gtgatggggt tgggatggct gcagtcggta aacttgcatg
      781 atgaatgaac aattccggcc gtgtcgggat tggtgaagcc tgagcgccgg aatgatatga
      841 gtcaagggga aatggtctat tgtaggaggc acgatgaaag ggctatcgaa gtatctgctc
      901 gccggcatga tgctgatttt caccggcgtt ctcatcgcct cgcccgttga acaggtgggg
      961 gtgatcctgc agccgatggg gctgctcaag gctgtcgagt ccgattccga tctggtcccg
     1021 gttgagtttg agttcaagtt tctcaaattc gccacggact cgggcgtcgg tatcaacggc
     1081 accttcaaca actggggtga tgtctttccc atgcgcctgg ccatacctct cgatggctgg
     1141 actctgacga tgaagctggc tccgggcagc tatgtctaca aattcgtcac ctacaccgat
     1201 acagtcggac aggcgggagt gaccggatgg taccccgatc cgctcaatcc catcacccag
     1261 ggtcccttcc gcgattcttt catcgccgtc agcgacccga tgatctatta tccctcgccg
     1321 atgccggatg cggatatcac cgattccaca cccctgctct ccttcaagat cgcccactcg
     1381 ccgcggcgga ccctggatcg cgatgcctgc gaattgatca ttgacgggat cccggtcgcc
     1441 aacgcgggcc gctattacag caccgtcacc catcgcctct cctatacgcc caaagagccg
     1501 ctcttcaagg gggcccatac agcgaccctg cgcatccgca cgaccgacgg cgccacagcg
     1561 gaatatacga ccagtttcaa caccctctcc ggagtgggcg acaccccggt aaccctgagc
     1621 ttcgacggcc gcagccccca cctcgttctg aacggggtcc ccgcggaggc caccctggag
     1681 tggatggacc tccccctctc tcatcccctc acggatccgg atggtgatgg cttcttcacc
     1741 cgcgagatcc gcatgaaact caacgatccg cactattacc gcgtcgtggt cgacaagcag
     1801 atctatctgg aattcgaccc cggcaatccc ctgctcaacg aggagtaccg cagcgtcgtc
     1861 gtcaaccact ttgaactgaa tggcgccttc cggtttctct cgccccggcc ggacaccctc
     1921 ttcgacggcc ggctgcaggc gttcaccatc cgcgcggcgg ctgtgcccgg ggattcaggc
     1981 tttgtgctcg atgaaagctc tctgcaggtg gaaatggacg gcgcggcagt accctggagc
     2041 ttgaataccg gcagggatcc cgtcgagatc gaaatcacag cgcccctcac ccccggccgg
     2101 cattttgtca agctggcggc gggggaccag ggcggcgagc ccttcacgcc gggctatctg
     2161 gccttcggcg tttaccccgc cggcagcggt ctgcacgtgg tcgatgccga gcgcgatgat
     2221 accggaccgg ggagctaccg ctatccggcg ggcgtcacag agggatgcgc cgacatccgc
     2281 agcgcccatg tcgcggtcaa tgccggactc gacagcctcc tcttcgagat cgagatgcgt
     2341 gcggttgcgg acgagacccg gctcggcttt ctcctctaca gccggctcgg caaaaagctg
     2401 accgatgccc cggaagccct ggagctgcag atccccgcct ggcaggggac cgggctctat
     2461 gccaccctgg cggcgccggg aggccagttc ttccggccgg accgcgataa tctgctcttg
     2521 gcggagggtg ctgcgccggc catcgccctc tcgctcaatg gctccgcgca ggaggaaaac
     2581 cgcttgcggt ttgcggtggc gctcgccgat ctcgagaagc tgatcggcag cttttccggg
     2641 gaatggcatg tgacgcttta ttccttcctg ctcaatgcgg ccggcagtat cgaactcgat
     2701 ggcgccagcg gcggggtctc cgctgcggac gagcccgatg tctacgacgt cgccttttgc
     2761 agcaacgatg aggtccaggc ccagctgctc gccaattaca tctatgccta caaactcggc
     2821 ggaccgcgca cagccgccat cggcaccgac ctgcgcggca gctggcctct gcagcccgct
     2881 gccctgcatc cccagctggg gaaggggacg ctgctgcggc tgcgcaccaa tggcggggat
     2941 atcttccgcc gggaagtgcg gcttgccggc gaggtcagcg atccggccgt cacccaggtg
     3001 caggtgcagg tcaacggcgc cgccgtgccg gctgcgatcc agggcggccg ctttaccgcc
     3061 gatatcaccc tgcagtacgg cgtcaacgag atctctgcca ccgcagccgg caccggcggc
     3121 catgaactgc actcgcagtc cgtccgcttc acctgccgcc tcgataccct gcctgacgtc
     3181 cgcatcacca ccagcgtgac cgaacagctg gtcaccctcg acggcagcgc caccagcgat
     3241 cccctcggac gcgagctgac ctacagctgg agcgccgatc cgcgcaatcc gcaggccgtc
     3301 gcctggagcg gcggggatgc ggccctggtc accttcgccg ctccggcagc cccgggcgag
     3361 tactatttca ccctcaccgc cacccccgcc ggcggcgccg ccgcctgggc gcggaccgtg
     3421 gtgctggtcg agggcggcca gccccgtacg gccgatctgg agaactggca tccggcctgg
     3481 atcgacagcg ctgtggccta cgagatctat acccgcgcct tcagccgcac cggccgcctc
     3541 gccaacatca ccagcaaact ggcggatctg cgcgatctcg gcgtcaccgt cctctatctg
     3601 cggccgatcc atccctccac cgccatccac ggctactgga tcaccgacta ttacggcgtc
     3661 aatcctgaat atggcactca tgcggatctc ggcaacctgg tcaggacggc ccacaaactg
     3721 ggcctccgcg tcgtcctcga ttacgtcgtc aatcacaccg tcgacaccca cccctacatg
     3781 caggatgcgc tggcctgggg ggaggattcg cccttttacg atttttaccg ctggaaccag
     3841 gacggcaact ttcactacta tttcacctgg gttaatctcc cctcgatcaa tttcgagtcc
     3901 ccgcacacgc gccagtacct gatcgatatg gcgtgctggt gggtgcagaa tttcgatatc
     3961 gacggcttcc gctgcgatgt cgcccaccgc atcgaaaagg accgcccctc ggggccgctc
     4021 ttctggcagg aatggcggcg ggccctcaag gcgctcaaac cggacctctg gctgctggcg
     4081 gaagccgatg cggacgacgc tgcctattac gacaaaaaat tcgattcggc ctacgattac
     4141 cgctgggtga acacggtcat caacctcagc aacaatctgg ccagcgccga acagctgcat
     4201 gaagtggtgc agtattatga ccgtccggag tttcccgccc atgccctgcc caaacgttcc
     4261 ctggaggatt ttgacaactc ccgcttcctc gccaacacca cagtcgagca gtccatgctg
     4321 gccgccgccc tgcagttgac cgcacccggc gtgcccatgc tctatgccgg ccaggaggtg
     4381 ggcgaggtga gcgaccgggg tctggtgagc tggagcgatc cccggcagct gaaaaaatat
     4441 taccgcagcc tggtgcagct gcggcgcaag aatccggccc tctcggtagg tgattacacc
     4501 ccggtgccga ccgatcagcc cttggtctat gcgatgatgc gcgcggacag ctttctggtg
     4561 gtggccaact ttggcagcag cgagctcacg gcccggcttt ccgtacccgc aggcctgatt
     4621 cccggcgaca gcctgaaggt cttttatctg aacgaccagc tggtcaagct gaacctgccg
     4681 gtgaccgccg ccgccctgcg cgcctatgac ctgtggcttc cggccaggac ggtgcgtatt
     4741 ttcaagctcg gcgccgccgc caccggcata gagaaagcgg aggagaggcc ggaggtgttc
     4801 ggattgacgc ccaatttccc caatcccttc aacagctcca ccgagattcg cttcgccatc
     4861 ggcggggccg cgccggtgac ggctgagctg gacatttaca acgtgctcgg ccagcgcgtc
     4921 cgtatgctgg tgcgggaaaa gagggggccg gggcgctaca gcgtgttgtg ggacggccgg
     4981 gatgaccgcg gccaggcgct cggcagcggg atctattttt gcagcttgaa ggcgggggat
     5041 tttgcgcagt cgcgcagaat gctgatgctc aggtgagcct tgcagttgaa tctgatggga
     5101 ccatcgggtc aactggtcgt aattgacccg atggggattt gtcgctcccg gttttagggc
     5161 atttccggat tatcgggtca ggcgcttgta tttgacccga tgggggacgt ccgcggcctt
     5221 gccgaggcgc tggcggcggt tttcctgata gtcgctgaaa ttgccttcat accagaccac
     5281 gccgctgtcg ccctcaaatg agatgatgtg ggtgcagatg cggtccagga accagcggtc
     5341 atggctgatc accacagcgc agccggcaaa gttgagcagc ccctcctcca gcgcacgcat
     5401 ggtgttgacg tcgagatcgt tggtgggctc atcgagcagg atcacattgg cgcactgctt
     5461 gagcgccagg gcgaggtgca cgcggtttct ctcgccgccg gagagctgcc cgactttctt
     5521 ctgctgatcg gcgccgataa aattgaactg ggccacatag gcgcgcgagt tcatctcccg
     5581 gtcacccagt ttgatgcgct cctctccgcc gctgatcatc tcccagaccg tttttgccgg
     5641 atcgagcgcc gcccggctct ggtcggcata ggccaactgc acggtctcgc cgatgcggat
     5701 gaccccgctg tccggctgtt cgtccccgac aaccatcctg aacagagtgg tcttgccggc
     5761 cccgttcggt ccgatcacgc cgacgatccc cccgggaggc agactgaagg tcaactcctc
     5821 gaaaagaatc ctgtcgccat aggatttgct gatctgctcc gcctgaatga caatcttgcc
     5881 cagacgcgga ccgggcggaa tataaagttc cagctctttc cggcgctcct cgctcccctc
     5941 ctgcaatagc tgctcatagg cgctgatacg ggctttggat ttggcctgcc gcgccctggg
     6001 cgtcatccgg atccattcca gctcccgctg cagggtcttt tgccgctcgg tctccttttt
     6061 ttgctcgagc agcaacctcg cctgcttttg ttccagccag gaggagtagt tccccttcca
     6121 ggggatgccc tccccctggt ccagctcgag gatccagccg gcgacgttat caagaaaata
     6181 gcggtcatgg gtgacggcga tgactgtgcc ggcgtagcgg ttcagctccg cctcgagcca
     6241 gagcacggtc tcggcatcga gatggttggt gggttcatcg agcagcagga tgtcgggccg
     6301 ctgcagcagc agccggcaga gcgccacccg ccggcgttcg ccccccgaga gcaccctgac
     6361 cggggtctcg cccggagggc agttcagggc atccatggcc agttccagac gtgagtccag
     6421 atcccaggcg ttcagctggt cgagctgctc ctggacctgg ccctggcgct cgatcagctt
     6481 gttcatttca tcatcactca tcggctcggc gaagcggccg ttgatgacgt cgtattcggc
     6541 aagaagatcg acgacctcct gcacgccctg ctgtacgatc tccctgacgg ttttggtttc
     6601 gtccaggaga ggctcctgct ccagcatgcc gactgtataa cccggcgaga gcacggtctc
     6661 cccgacaaag tccttgtcgg tgccggcgag gattctcagc agggtgcttt ttcccgagcc
     6721 gttgaggccg agaacgccga tcttggcgcc gtaaaaatag gagagataga tatccttcag
     6781 aacggctttc tgctggtaga tcttgctgac gccgatcatg gaatagatga ctttgttggg
     6841 ttcgttggcc atggcaacgg caggtccgat ttgggttcgt tttgacggca atttagccag
     6901 gctggcaatg agaaacaagc aatttctccg cagcggcccc acggctacag aaatttcaac
     6961 cctgctccct gggagaaaaa aaaaatcccc gcctcccttg cgggggacgg ggacgggctt
     7021 gaagggccgg aaaggttact tgatctcttc cggattcatg gtgatctcgg tgaaaagctc
     7081 cgagtagaag gccaggggcg cctcgggatc cgcaatcctg attttggtat ggagcgaatc
     7141 gctgttgaga taggtcaaga tcagattggc ctggacgcgc accagggggt ccgcatcctt
     7201 gcggctgatc cgggcgagat cgccgttaaa gccggagatg ttcgcctccg gatagcgcga
     7261 cctgatcagc gccagcatgt agagggcgct ggaacgcacc ccgggattgt ccgatttcag
     7321 ggccatcttg taattgtcga ccgcaaccgc cagcctggca ctatccaggg tcgtcgtgct
     7381 gtccgctgca tagctgaagc cagccagcag gccggccagc accagggtca tcattccctt
     7441 tttcatatca acctccttgc atagggtctg ttgaagggtg taaccgcaag aacggcgcgc
     7501 tgccgttctt cgtgcccatc cttagcaaag agtctgccat aattgtacaa ttgccgtaat
     7561 ttatttattt tcaatacatc caggcgagca gagcgtccgg cgggcttcgc aaaggggtgc
     7621 cacgtttcgg agggattggg agtgaaaata acgtctattt atttatatta tttatcatta
     7681 ggatcctgcc acacttcgcc ggcaggacgg aatcgtggca ggggccggca agtttgcagc
     7741 cgcgctggca gagattacgg gggccgctga taaaataaca gcgagcggga caaagcgtcc
     7801 tgccggcggc ggactggtaa gctcagcacg gggccggccg ccgtcatttg ttgcgctgca
     7861 gacggttatt gagggcgcgg cgggtcaggc cgagcatgcc ggcggcgatg ccctggttct
     7921 gctccgcccg ccgcagggcc tcggcgatca gggcctgctc tgctgcgcgc agggtcggca
     7981 gggtcttgcc gaaggagatc gactcctccc cggcctcttc gcccccgtgc tcagccggct
     8041 gctcggtctc atcccagccc ggctcgatcg cgcggcgaaa ggcggtcagg gagagcacac
     8101 cccccttgtg gcggctgacc gcatcgaaga tcatcccctc gagctcgcgg atattgccgg
     8161 gaaaggggta gttggcaagc agggtataga gttccctggg nnnnnnnnnn gcggcgtgat
     8221 cctgcgcctg tccagctttg cagcggcctt gtcgaggaaa tggtcgacca gcagggggat
     8281 gtcctcgcgg cgctggcgca ggggcggcag ggcgatatgg tgggatttca gccggtaata
     8341 gaggtccttg cggaagcgct cgctcttttg cagataatcg ctgtcgcgca gggtggcgac
     8401 cgttacgcgt gcgtcggagg ttctgggctg gtcggcgccg aggggatagt actcgccctc
     8461 ttgcagaagc cgcagcagct tgacctggga ttcgagactg aggtcgccga tctcgtcgag
     8521 gaagagggtt ccgcccgccg cctgttcgat cagccccttg cgttcgcggt cggccccggt
     8581 aaaggccccg cggctgtggc cgaagagggt gtcggagaaa aaatggtcat ccaccccggc
     8641 gacgttcagg gcgacaaaat cgcctttgcg gccgctcaga tcatggaggg cgcgggcgat
     8701 cagctccttg cccgtgccgg tctcgccggt gatcagcacc ggcaggggcg tcgcggcgat
     8761 ggcctcgaca tactgaaaga tgccgcgcat ggccgggctg tcggtgatga tggcggcgaa
     8821 gcgttcggga tgttccaggc gcgcggagat caggtactgc ctgagtttga tgttctcgtc
     8881 gcgcacctcg cgcagctgca gcgcccgggt caccagtgtg accaggcggg cgtcgtcgac
     8941 gggcttgacc gcataatcaa aggcccccgc cttgatggtc ttgaccaccc attccacctc
     9001 attgatcgcg gtgatgatga tgaccgggat gtcgggaaaa tcccgcacga tcagtttgag
     9061 cagctcctcc cccgtcaggt gcggcatcat catgtcgagg acgacgaccg aaaaggactc
     9121 ttccgccagc attcccatgg cctggcggct gtcctgacag ctgcgcacat tgttgatccc
     9181 ctcggagatc agggtgtagc tggcactgcg cagaaaattt tcctcgtcat cgatgaggag
     9241 caccggccat tctggataga gtgctgaatt catgggcatt cctctttgct tgcggggtca
     9301 gggcagcggc aggatcacac gcacggtggt cccctcgccc ggtcttgagg tgaagagcag
     9361 ctcgccgccg tggtgtttga cgatgttgta agaaatcgcc agcccgagtc ccgtgccgcc
     9421 gatgtcgcgc cgggtggtaa aaaagggatc gaatatatat ctggcctgtt cgggatcgat
     9481 gcccttgccc tcgtccgcca cctcgatcac agcacggtgg cgcgcccggt cgtgtctggt
     9541 gcgcagggtg atcgatttgt cacggctggt cagggcctgg caggcgttgg tgaggaggtt
     9601 gatcaccacc tgctcgagct gctggaaatt cccgcgcacg gcggggatgg agtcacccag
     9661 ttcgacggcg aaatggtcgg tggatttgcg gagcaggttg gcgacgatca tcatcgcggc
     9721 ctcgacgacg gcattgacgg tgaaattctg gttgagttcg cctggatcgc tgcgggcgaa
     9781 atccttgagg ctctggacga tcttctggat gcgcaccgtg ccgtcgacga tggcctgggt
     9841 cagctcgggg atgccggtga tcgccctgga gtagggcatg ccggcgatgc ggaaatcccc
     9901 ttcatggaga aaatagtgat gcatggccgg caggagatcc ttccaggcct tggcgatcag
     9961 cttggcgttg agaaggataa agttgttggg attgttgatc tcgtgggcca cccccgagac
    10021 cagggtgccg agggtggcca tcttgtccgc ctggatgagc tgctcctgct ggcggatggt
    10081 cagctcctcc gcctgcttgc gcgcggtgat gtcgttgatg aggccgtcgt actcggtcac
    10141 ctcctcccga tcattgcggt gggcgacgac gacgctcttg acccagcgca gggtggtgtc
    10201 ggcgtggatg atacggtgct cgaccggcag ggttgcctcg ccgcgcagta ccgcctcggc
    10261 ctgggcctgc accgccggcc ggtcctcctc cggcaccatg cggatccaga gggtgggatc
    10321 atcgcggtac tcctcggggg tataaccggt cacgaggacg cagccgggac cgtgcagggt
    10381 ctcgaccgcc cggccgtttt caacgcgcac agtatagata taatcgaccg ccgcctggag
    10441 aagccggcgc acgcgctgct cgtccctgct cagggcagac tcggcatccc gcggccgccg
    10501 gcgcccccct gccgcggtct tgccgctggc gttcgtatta ttcaccttcg ccgcctccgc
    10561 tcagatcgcc tgtgccccgg cggcgaggag aaaaaccacc gcgagctggg tcaggaatcg
    10621 cttcatgatc gcccctcgtc cagttcggat tggaacaatt attgcaaatg gtgttcgcaa
    10681 gaaaaaatcc gcccatctca gcacagttct taaaggagtc agcgcaccca tgggtcataa
    10741 tcatcacact gctgtcaccg gaaagggaca ctcacggaaa ccatgcctgc tgctgccgct
    10801 cgccggtctg gtcctgcttt cgtttctctt cgcccggtcg gccgccgccc agcctcggca
    10861 tgaacgcctc atctacagcc atcccctcac cagcgcagcc gatgatcagg gtctgctcct
    10921 gcgcagcacc gacggccgct ttggcgcctc cgggtggcag agtctcagcc ccggcagcca
    10981 gctgctcatc acccttcccg agggtctgcc cctcgagggc acctgctccg tgcgggtgag
    11041 caatttcgat ccctgtgagc agaatgtcag tgaaaagcag cccatcatca acctctactc
    11101 gcagccctgc ggcaacaagg agatctacga gaccgacggg ggctggtttc accttatcag
    11161 tggtaccctc taccagacgg gcgatgagac cgccggcttt aaattctggg cggccccgcg
    11221 cggggtggaa agcaagaccg aggaggaggt gatgctcgaa gcccgctgga accccgccag
    11281 gagctatgaa ttcaccttta tctgggacag gagcaccctt ttcttcctcg tcgacggcgt
    11341 cgagcgcatg cggctcgctt acgccggcca ggtggagccc ttccgctaca tttttcttgg
    11401 caaggataat ctcacccggg gttacaccgc ccagcccggt ccggtttttt ccgaccttcg
    11461 cctctacgct cccgcggagc aggacccccc gcccgcaccg ccggccggtg tgcgggtcat
    11521 ccatgcggaa taacagccgc accattctcc gtcagcggaa tcaggtcggg ggatgcagcc
    11581 gtttctgaaa ccaggggcgc agcttgcgca tctgctcgag ctgccccgcc ggggcccggc
    11641 ggcggcagct tttgatctcc tcgctccccg ccggggcgcc ccagcggatc tcgaccccgt
    11701 cgttgggatt gtagcccatc tcgaaggcct gcgcgcgccg cagtatctcg gcgagggtga
    11761 gctgttgttt cgaaccatcc ccgcgcgtat aggtgatggt catttccccg ctcttttccc
    11821 gcgtcagttc ctccagctca gccctgatct tttgaggatt cttccatctg gaaatcttat
    11881 agttccccgg cgcgcgcacg atgcgcgccg gaaagtcgag cacggcatcg atggcaatga
    11941 gcagccgcag gtcgcggttg ggagtggagt aatcctccca gagccccccc gccagaaaga
    12001 ccccggcggc gctgcccggc atggggatga ccgcaccggg atgggcctgc atccacttct
    12061 cgccgttggc gactgactcg acgcggacga taagctgctc ctgcaaggcc ctgatgagct
    12121 ccagcagggc cgcctccgga tcgagcccct ccggattgat gagcctttcc atggtgtcat
    12181 aaaaatgttc gctgctcatc cccttctgct cgagcgagtg ggcgccgtaa cccggcgctg
    12241 cggcgatctc gcggttgctg agcagccgcg gctttccccc ctcgacgcga atgggccgga
    12301 aggccttgaa gcccggctcg ccgatcaccc ccgtggtggt gaagagaaaa ttgcccttcc
    12361 agaaacgctt gatctgcacg gtgccgtcgg gctgggcgtc gacggcgaga aggagtcccg
    12421 gcttgcggcc cgactggggg acccagcgca ccagggtgaa ggtatggccg taggggtcgg
    12481 cgaagaccgc gccggggcgc agggccgccc gggtgagggg caggggatag taatccgagc
    12541 tctcatcctc gaggcgggtg cgcgcggcgc cggagtggat ggtgttcatg acctcgcgca
    12601 gaaaggcgtt gaaggcgcgc acgggatggg gctggcccgg gtgcgaggtg ttggtgatcc
    12661 agcgcccggt atgggggggc tgcttgaggg tgccgcggcc ggtttcatga aagccaaagg
    12721 gcaggccgag cttccagctg aaataggcgc gcagatagaa gggattgtcg gcgcagtcgg
    12781 gttccatgat cacgctcacc ttgccggccg catcgtcctc acccagcccg agatgattga
    12841 agaggggatt ctcctccgcc cggcgcgtca cctcgtgcag ggagggccag gaactccctt
    12901 ccggcgcccc ggcgaagagg gcctcgatcc aggccgcata gaggagttca ctctcgcgcc
    12961 cccaggcgcg gcggttctcc cagactcctg cggccggttt gcgcaccggc gcgggcccgg
    13021 gcgtgatctc gacctcgcag gaggccagaa tcgtgcgccc cgacttgagg gcgacctggt
    13081 agcgtcccgc cgccccggcc tcaaagccgg cgcagcgcca ccaggggggg ccgcccccct
    13141 cgcggccggt gcggacatcc agggggccac cgggaccggt gacgacgatc tggcttttgc
    13201 ccggatcggc ctcccaggcg gcgaggacgc ggaagctccc gccggcagcc ggtcgctccg
    13261 gagagaccag caggacgggg ttggactgcg ccgcggcgga gaacatcccg cttagtacga
    13321 gaatgccaat cagatttctc acatcatccc ttatttcaga tagagcattt tgcgggtcag
    13381 ggtgcggccc gggccatccc ctggggagat gagcagccgg cagaaatagc agccgccggg
    13441 cagtcccgcc tcccctggcg caaagcgggc ctcgtgcgca ccggcctgtc gcagcccctc
    13501 ggccagcagc gccacgcggc ggccggccag atcaaagacc tcgagccgca cctgcgcggg
    13561 ggcggcgaga tgatagcgga ccagggtgga gggattgaag ggattgggat agttctgctc
    13621 gagaaggaat attccgggcg gctgcacgcc cggttggact cggctcgtct tctcctgcac
    13681 cttctcctca ggggctgccc cgccgtccca gcagcgccac tcggacccca gtttgtagta
    13741 aaaacggttg aggttgtctg tgagctggct gtcgaaggcc agccagggcg aagcgctgct
    13801 gcggtggacc tcgacggcga ggatgttctc gccgctgcgc agtttgccga gcaggccatt
    13861 ctggaggttg agcaccacca tgctgctcat ggccgcgctc ttgctggcga agagagaata
    13921 gccgggggcg ccggccggca tgttaatgcg ctgaatctcc tcgccgttga gccagacgat
    13981 ggcaccgtcg ctgcacttga tcagggcgcc catgccggcg atggcggaga ggtcctcgat
    14041 ggtgaaggtg cggcggaaat aggcggcatc gaccggggcg atgagagtgc gaggggaggc
    14101 ggccgcggaa ctgccgaggg gcgccggccc cgttttccag gcgctgtcgt cgtactccag
    14161 ctcctgccag cggcgcaccg cccgggtggt gtcgacggtg aaccggagca cagcggagga
    14221 ggccgtggcg ttgccggcgt catcgatggc gtggagatat agggtatagc tgcggccgtg
    14281 ttcgaccgga atggtcgtcc ggtgcagccg gcggccggtg gtggcgaagg tgtgctccat
    14341 ctgcgcccag ggccggtcct cttcgctcca gcgcacgctg gcatagctgt cggtctccac
    14401 ggtcagctcg aggagcgggc tgtagagggt gaagggctca tcggccggaa gggctgtgaa
    14461 gcggggcggc gtcacatccg cctcctccgg ccagatgcgc aggctgtcga tggcggcgat
    14521 gagccggccg gcgatctggt ggtgagtggc gacattggga tggccgccgt tgacgtagcc
    14581 ggtgtcgaaa taggggaaat cggcatagtg gatgcggccc aagccggcgg cctgcgcctc
    14641 ccggaccacg gcggcggtca gctcctgcaa gagctcgaca tggggggcga aggcgaggat
    14701 cgtggcggcg ggatagagcc cggtcagccg ggcgacgaat tcgtggtagc ggttcttgaa
    14761 gagggtctgg ttcccggcgc tgaccccgcc gctccagccc ccgaggcctg aatagtcgtt
    14821 gaggccgaga cagattacca ccaggtcggg ctgccaggcg gaaaagtccc atttgatggc
    14881 gctgttatag acgaccgccc ggtcgaaaag ggcggggatg gtgccggtgg catccccctt
    14941 gtaatccacc gccatgccat agcccgagat gctgcacatg tggtactggg cgttgaaatg
    15001 gcgggcggtg atggggcaaa agccctcgaa aatattggta tagggggcgg gatcgggcgg
    15061 ggtgtcggta taggcgtatt cgttgccgga agcgctggtg aatgagtcgc cgatgaactc
    15121 gatgcgccgc gccggctttt cgggcggggg cagcagatct ctgccctcat cgaggaggag
    15181 gccgttgaag gtgaagcggc tccaccagta ttcattgcgc ttggtgagca gcaaggagtg
    15241 ctggccgtcg gggagcccct ccaccacgct gtagctggtg gtgccggact ggctgccgtg
    15301 gaagatggtc actggttcgc cgtcgatgaa gaggttccag taataggcgt cgtcggtcat
    15361 gcgcagcccg aggctggtgc cctcaaaatc ggcgcggata tagacccccg gccagccgtg
    15421 ggcgggagcg aggggatcgg tgaaatccca gcggccgaag tactggatcc aggggtgatc
    15481 cgccggaaca agccggctgc cggcgagggg agcggccttc gccgcggccg tcgccgctcc
    15541 ggccggttca gccgctgcag tcgggatcac cgttgcggtg gccagggcaa tcgaaagcgc
    15601 gatgcgaatg agattcatat aaattgctcc agcctcattc ctggcccggt gtgaaggcat
    15661 cgtcgatcca gatggcgagg gtttcgcccc agtagatgcg gtgataatcc ttttgaggat
    15721 aattgccctc gactccaggc tggaggaagc ggccgggatc gaaatcctga aaggccaggg
    15781 tgcggcactc gaaggagagg gcggccccgg cgacgcgcgg cacggcgata gtggtgctct
    15841 cctggggcgt cagtccggcc ttttcccatt tattgatgtg gcggccactg tgggatccgc
    15901 agtaattgag ggcgccatga aaccgggccg gcatgacgtt cagggtgaac tcggggtgct
    15961 cctcgagcag ctgccaggta tagcgggtcg ggcgcacata gagggtgacc acgggcttgt
    16021 tccagagggt gccgaagccg ccccaggaga cggtcatggg attggggtgt tccaccccgg
    16081 cgaccagcag cgcccagctc ttgtcgagga tatcgaaggg cgaaatcacc agttcggagg
    16141 gattgagttg tttgagcatc tggtcttcct ttctgtgcgc gtgtgtcccg gcaggtgcgg
    16201 agaagaaaaa atccgccatt gttcttcctt caacccggtc tcataaagat atcaagattg
    16261 cggcttaaaa acaagagaca atcgaggcca tatcagcctt ctcgctccca cctaccggtt
    16321 aatctcgctc ccacctgccg gttaatctcg ctcccacctg agccgactct caccatctcg
    16381 ctcccacctg ccggttaatc tcgttcccac ctgagccgac gcccacaata aggctgggat
    16441 cgtagcaaaa aaatccag
//
LOCUS       DAOPWU010000033        16259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3647_length_16259_cov_5.401753, whole genome shotgun sequence.
ACCESSION   DAOPWU010000033 DAOPWU010000000
VERSION     DAOPWU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16259
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3647_length_16259_cov_5.401753"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKM23_03350"
     CDS             <1..499
                     /locus_tag="PKM23_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNY90541.1"
                     /translation="PMAEAAKIGDLFCTLTGDLHVLRAEHFAVMKDGAIMANSGHFNV
                     EIDIPALKELAIASRQGVRNFVDEYTLPSGSRVRLLADGRLINLAAAEGHPASVMDMS
                     FATQALATEWVVKHAGGLAVQVHDVPEEIEDWVARLKLKSMNIEMDELTEEQKQYLAS
                     WEMGT"
     gene            496..1017
                     /locus_tag="PKM23_03355"
     CDS             496..1017
                     /locus_tag="PKM23_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90542.1"
                     /translation="MSLHQENRPGWRRPGLVLFCAGLIALCGCKREPERQQEIRQVEL
                     EAAPVSEAQLARLAGDLLSLQEQIREAPASIELRERLLALALDEENKSFRAVGFGLIP
                     ENPESRETVQKSTERAALLDGCRWLAYLRAWRKDIKKPDFGAIRGEVPPAKTIYQHTS
                     VDQVVMIVETEAE"
     gene            1014..2264
                     /locus_tag="PKM23_03360"
     CDS             1014..2264
                     /locus_tag="PKM23_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90543.1"
                     /translation="MMRGAGGWVILVLICTAAAFGQGRSAGMHGAYVTLATGAEAPYY
                     NPANLAFTRGRQTTLSLLGVSGWAADNAFGLEEYRRYNGAYLDQAAKEEILAAVAGEG
                     LLARAGVTARPFSIAVGPAALSWQTRAVSRLSLAEELFHLLLEGNEVDKTYDFTPAAG
                     RAMLLSDLAFSWGYSPPFTLRGIHSISLGINARYIIGSFYGQVERMQARALTGATLAR
                     AEGEATLRTAQGGRGWGLDLGMTAVVNPHLRFGLFSENLASGMSWNRESERIQADFTL
                     HATNIDRILEEDLDLEEVVMHSDTTLAIPAFSLRLPRRLHFSGSYLQKRWSLSAEWSG
                     GETGGGDWGGAEVALGGEFSPWRFLWLRGGAGHAEDLGWSSSLGFGLQMLGGRWDFAW
                     QGVGSLHPGRVRGGLVATSLFLIL"
     gene            complement(2343..3638)
                     /gene="pfp"
                     /locus_tag="PKM23_03365"
     CDS             complement(2343..3638)
                     /gene="pfp"
                     /locus_tag="PKM23_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNY90544.1"
                     /translation="MSAKKKVKTGNLAILVGGGPAPGLNGVIAAVTIEARRHNLKVYG
                     IMDGYKWLVKGDEAEFKANVKELRISDVSRIHYDGGSILRTSRTNPAKSPNGVENSVK
                     LLQSLGVSYMVTIGGDDTAFGAMEIARAAGGQIKFAHVPKTIDNDLPLPNNLPTFGYQ
                     TARDLGATLVKNLMEDARTMDRWYITVAMGRHAGHLALGIAKSAGATLAIIAEEFGHE
                     GFIPLDHICDIVETAVIKRRAMGNRHGVVVIAEGVAERLSPEELKQIPGVIVEYDSFG
                     NILHSEIELGKIIKLKVESRFAARGEKMSMVEINIGYVLRCADPIPFDQEYTRDLGFQ
                     AVRYLLNDNNIYKGNAMISVDNGKLHPIPFEEIIDKETKKTAVRLVDVTSDSYRVARS
                     YMVRLEKRDLEDKELLPRLAAAARMTPKQFIERFEYLAK"
     gene            complement(3719..4597)
                     /gene="pheA"
                     /locus_tag="PKM23_03370"
     CDS             complement(3719..4597)
                     /gene="pheA"
                     /locus_tag="PKM23_03370"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNY90545.1"
                     /translation="MDGLMASDPDAAPATVAFQGDHDAYSELAAFKFFGENASCVPCA
                     LFEQVFDAVISGTTRYGILPVENSSTGSIHVNYDLLLQHEVAIVGEVYYRVDHQLIAH
                     AGAALQDLRVIYSHPQALEQCRQFIASLPGIRAVPALDTAGSVRMILEQNLRDAAAIA
                     SRRAAALPGMQILRENIQDIAENYTRFLLISRESLVPAEADKTSIVYAMKNIPGALYK
                     SLSAFALRDIDLCKIESRPWRGRPFEYIFYLDFRGTPAQQRCRNALRHLEEMTSFLKI
                     LGSYPEGEGLNPPLSR"
     gene            complement(4640..5650)
                     /locus_tag="PKM23_03375"
     CDS             complement(4640..5650)
                     /locus_tag="PKM23_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNY90546.1"
                     /translation="MPIPTVPAREESVHVLALALIAAGILLPLLICALAAPPLFRLLL
                     FTLWLNGLASTLPPQPGRRLCLGALLALLLLALHQLFAPLLPGLIASAPPGSAAPALH
                     GDAALISRHLAAGRSELLRFARQLTALLWPSTATLALALLLAGLLTALRPPLHRELLR
                     LAPNRALTRRAWLLRELPDRFGRWIRDESLRSLLMGGLWALGTWLLGYSHPAAAGLFM
                     ALAAPTPFWGPLFAAGLSLFFALDPGHLPLQAGGAAIVFAVTWLAAHLLLTTRLALSR
                     PPLSRGWTLLWALAGYLCAGVGGFFIAAPLIAAARHADGALHLPTPRSCEPTSPQSVQ
                     FS"
     gene            complement(5680..6198)
                     /locus_tag="PKM23_03380"
     CDS             complement(5680..6198)
                     /locus_tag="PKM23_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNY90547.1"
                     /translation="MERTNKKPEKEAGQPCRPEEIEYLHALNRLTPAGRKRLLTWLHV
                     RTYAADEPLFRQGEPALCLFFLLSGSLALEADSRTGRPDRLKIVAPGQACGSAALAGD
                     AFYGESCLALEPSRVAVLPQTAFHQLLAGQPAIAAALLQGLLEETLDDWRQALRTYSG
                     LTDHLTRANIIV"
     gene            6503..7609
                     /locus_tag="PKM23_03385"
     CDS             6503..7609
                     /locus_tag="PKM23_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNY90548.1"
                     /translation="MQHKTELQNLREEIKRQEKALSRRKKDEKSAIDYLAKLDREIDV
                     SHAYLHSLNGDVDRLGRQVDERDGRIRALTAEKEALAEQVKKRLVSFYKHGRAQEIDL
                     MLSIRSVQQAQVWMRYQKMIADADRRNLQALAARRQELEKQQDYLRQEKADKARALRE
                     REAEERELTSSREKRRIYLASVRKDKKVLEQRLRDSRASEKQILALIARAEQARLTAS
                     ARKPAAAKGRTQPPVSSKFAGMKGRLSWPVRGTIVSRFGRQRHPELNTITENLGIDIK
                     TRQGGTVVSVGDGQVQTITWQRGSGNIVIVSHGEGFYTVYTHLEEIRVSQDDPVRQGQ
                     VIGTVGESGSINGPVLHFQIWKNTRNLDPEEWLG"
     gene            7650..8744
                     /gene="holB"
                     /locus_tag="PKM23_03390"
     CDS             7650..8744
                     /gene="holB"
                     /locus_tag="PKM23_03390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNY90549.1"
                     /translation="MSFAQIIGQSRPKEILARALQTGRIPHAYLFAGPAGVGKEAMAI
                     EMAKALLCSGSDDKPCDGCSACRRAGRFGHPDFFFLFPMPKSAAVEEEREVLDSLVRD
                     PYVRKQPWASPTIGIERIRELRRVSSLKPLEGRRVVIIAEADKMTPEAANALLKILEE
                     PPADMQIILTSSRDAGLLPTIISRCQRVDFNLLTNAEVAAALVEKKSVPEEQARLVAR
                     IAQGNYRHAAELLEENLQERRNLVIEALRISLKDEGTRIEWAEGVLDELDKRELRDFI
                     SLMLIWFRDALVLTANPAAVASDQLVNVDQIEVMSKFVAAFSEIRFEGIFTDLEKSIE
                     MLDRNVHPTLILVVMLGRLNRNLILKGSAL"
     gene            8741..9559
                     /locus_tag="PKM23_03395"
     CDS             8741..9559
                     /locus_tag="PKM23_03395"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HNY90550.1"
                     /translation="MMVEIVFKGERKDIYANPQEFPFQVGDYAIVEAEKGEDLGIVNQ
                     LGSVLERKKSDAVLRNILRKPTPRDLDRYRQNRVKEFEAYQICRKRIAETGLEMKLVD
                     VEYQFDNNKVTFYFTAEKRVDFRELVKYLAGLFKVRIELRQIGVRDEAKRVGGYGTCG
                     RKLCCTTFLKEFEPITTQCAKEQNLPLNPQKLSGLCGRLLCCLMYERDFYRRQMSRLP
                     RVDSRFQTPLGEGRVTHVDVFNERITLSFSPEKQESFDLAEMENFTLISAPEAE"
     gene            9639..11840
                     /gene="metG"
                     /locus_tag="PKM23_03400"
     CDS             9639..11840
                     /gene="metG"
                     /locus_tag="PKM23_03400"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNY90551.1"
                     /translation="MEERTINMSQEQQKEYKRLLVTSALPYANGPIHLGHLAGAYLPA
                     DVYVRYQRLKKRDVVYICGTDEHGVPIAIAAEKAGVTPQQIVDKYWADHYDTFTRFGM
                     SFDHFSRTSAPMQHRTAADFFKVIHDKGFLVERAIEQFYCEKCARFLADRFVEGECPR
                     CHRPGARGDQCEACGSSLEQTELIHPYCKVCGGTPVLRQTRHLFLKLNEFQPLLEKWL
                     ADKGEWKENVLNYVRGWFQEGLGERAVTRDLSWGIKVPVAGYEEKVIYVWFEAPIGYI
                     SATKEWAEKKGRPEAWKEYWLQPDTKLVHFIGKDNIVFHAVIWPAMLMGHGGFVLPAD
                     IPANEFLNLQGEKLSTSRNYAVWLGDYLNKFPADPLRYYLAVNAPETRDADFIWEDFQ
                     GRNNSELADILGNFINRTLTFARKQFGGAVPERGVLDGLDEQMVRTLAEAPEKIGALL
                     EGYELRKAAAALIDVARFANKYFNDQEPWRTARENRAKCATTIHLAIQACQTLAVLME
                     PILPFSAEKVWAMLGQPGSVHDQVWDGCGSRHLAAGHLLGQEQILFTKIEESQIRPEV
                     ERLRAISEALKAASAGAEVPAEKADSEAPARAAAVGAGQPAAAAGPAAQGNPSAAAAE
                     KPRITYDEFAKVDLRVARVVKAEKVEKADKLLRLELEVGSEMRQIVAGVAKHYTPEEM
                     TGRTIIIVANLQPAVIRGIESNGMLLAAEDGQGRMAILSPQGEIDSGAKVK"
     gene            11837..12616
                     /locus_tag="PKM23_03405"
     CDS             11837..12616
                     /locus_tag="PKM23_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNY90552.1"
                     /translation="MNPWLIDTHAHLDFDQFDADREEMLERAWMNGVQQIITIGIDLA
                     TSRKSVALAEKHAMVYASVGIHPHDVARAGAEDLAEIESLLEHPKVVALGEVGLDFYY
                     NYSPEEVQRKYLRLFLDWSLEKERPLIIHTRDADEAILSILRERSRAGWQGVFHCFSG
                     DARMAEKVLEMGFHISFTGNITFKNSTSAGVMRGVPLERLMVETDCPFMAPVPHRGKR
                     NEPAYVQLVAQKIAEAKGIAFDQVAERTTANARALFNLPGV"
     gene            12675..14141
                     /locus_tag="PKM23_03410"
     CDS             12675..14141
                     /locus_tag="PKM23_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90553.1"
                     /translation="MKRGNVCLFAAGLWTALLFNMPGEGNAQQKMPPQDFFEFSTSYF
                     DEGEDHVAIYIGGFRKKEFLYGYHDAWGTYVTEYKETKIDRSDEIWKYNFRTQEWVQL
                     KPYDSSWLKVPPMAGHQALQNKREGYIYCFGGRSANGDSAVYMLNMQKREWRQLAFHK
                     FYDKEKIAAAAHFTPFTDGGKQFGGRAVFHGGVDNNGSVSSMLYQADFNGEEIQFTEK
                     QTPRALYGHSAIYDPGAETWHFWGGQYEGGINYENYQYNPKSGFGWGPPVQGPFVMPR
                     ASAATLLDGSTLYAWGGKGYTFLRKGGAAVGEEIRDDLISITARDGQLYSRIVATGLP
                     PASQASLSMEIVDGDTLFYLFGGISTIWSSGDTSVNNTGYRYSLTQKSVQQFDTTTAQ
                     WGRLQTSVEAERFPVSPELRLEVPVWPNPAGRTVSFALAGGSAIRSVKLYNELGQLVL
                     QIHHPREQRLDLSRCPAGLYFLRVDTESRPYFAKIVKK"
     gene            14160..15638
                     /locus_tag="PKM23_03415"
     CDS             14160..15638
                     /locus_tag="PKM23_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNY90554.1"
                     /translation="MSGELLLLTAAVIVVVSVLLSKVSERFGVPTLLLFLGIGMVAGY
                     DGPGGIYFDNARMAQTLGTIALAYILFAGGLETDVAAVRRVVRPAVALATFGVFSTAL
                     ITGLGIHYLLGHNWMESLLIGAIVSSTDAAAVFAVLRSKNVGLKEPLRPLLELESGSN
                     DPMAVFLTIGMIALIREPARPVLSLIPLFLQQLIIGLALGYLMARFFIRIINRVKLDY
                     EGLYPVLSLGWVLLTYALTAWLKGSGFLAVYITGLVLGKESFSHKKSLIRFQDGIAWL
                     MQILMFLTLGLLVFPSQLIPVAGQGLLVALLLILVARPAAVFISLLPFRFSFREKHLI
                     SWVGLRGSVPIVLATFPMLAGLDQTHFIFNLVFFIVISSVLLQGTTIPLMARLARVDA
                     PLVRQRRYPIDFDNPEGMDLQLVDLFVPYGSEAAGQQILELGFPRDCLITLISRNEKF
                     IMPGGSTVLEEGDVLLMLSSPENLAVAREILEKRREEKPPGG"
     gene            15726..>16259
                     /gene="citD"
                     /locus_tag="PKM23_03420"
     CDS             15726..>16259
                     /gene="citD"
                     /locus_tag="PKM23_03420"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HNY90555.1"
                     /translation="MEYLTGRAGRDEKSDLVVEIEMRREGGLEIQVNSSVKALFGKAI
                     TATAAGTLQELGIGHARVTINDKGALDWVIRARIEAAARAAGYKSGLKPLAVAPHPPA
                     DRNRLRRTRLYLPGSNPDLMLNAHLFGADCLILDLEDSVAPVEKFATRILVKHALAAL
                     EFGSSERIVRINPLQTPY"
BASE COUNT         3031 a         4780 c         5489 g         2959 t
ORIGIN      
        1 gccgatggcc gaggcggcca aaatcggcga tctcttttgc accctgaccg gcgatcttca
       61 cgtgctgcgc gccgagcatt tcgccgtcat gaaagacggc gcgatcatgg ccaactcggg
      121 ccatttcaat gtcgagatcg acatcccggc cctcaaggaa ctggcgatcg cgagccgtca
      181 gggtgtgcgc aactttgtcg atgaatacac cctgccctcg ggcagccgcg tgcgtctcct
      241 cgccgacggc cgtttgatca acctcgccgc agccgagggc catccggctt cggtgatgga
      301 tatgagcttt gccacgcagg ccctggccac cgagtgggtg gtcaagcatg ccggcgggct
      361 cgccgtgcag gtccatgacg tccccgagga gatcgaggac tgggtcgccc ggctcaagct
      421 gaagagcatg aacattgaga tggatgagct tactgaggaa cagaagcagt atctggcatc
      481 ctgggaaatg ggcacatgag tttacaccaa gagaaccggc cggggtggcg acgccccggc
      541 ctggttctgt tctgtgcagg gctgatcgcc ctctgcggct gcaagcgcga gccggagagg
      601 cagcaggaga tcaggcaggt cgagctggag gccgcaccgg ttagcgaggc ccagctggcg
      661 cggctggcgg gcgatctgct cagcctgcag gaacagattc gcgaggctcc ggcctccatc
      721 gagctgcgtg agcggctcct ggccctggcg ctggatgagg agaacaagag tttcagggcg
      781 gtgggtttcg gcctcattcc ggagaatccc gaaagccgcg agacagtgca aaagagcacc
      841 gagcgcgccg cactgctgga cggctgccgc tggctggcct acctgcgggc gtggcgcaag
      901 gatattaaaa agccggattt cggcgccatc cggggcgagg tgccgccggc gaagacgatc
      961 tatcagcaca cctcggtcga ccaggtggtg atgattgtgg agacggaagc ggaatgatgc
     1021 gtggtgcggg tggctgggtg attcttgttc tgatctgtac ggccgcggcc ttcgggcagg
     1081 ggcgcagcgc cggcatgcac ggcgcctacg tcaccctggc gacgggcgcg gaggcgccct
     1141 actacaatcc cgcgaacctg gcctttacac gcgggcgcca gaccaccctc tccctgctcg
     1201 gggtgagcgg atgggcggcg gacaacgcct tcggcctcga ggagtaccgc cgctacaacg
     1261 gggcctatct cgaccaggcc gccaaggagg agattctcgc tgcggtggcg ggcgaagggc
     1321 tgctcgcccg ggccggcgtg accgcgcggc ctttctccat cgccgtcggg ccggccgccc
     1381 tttcctggca gacccgcgcc gtcagccgcc tctccctcgc cgaggagctt ttccatctgc
     1441 tcctcgaggg caatgaggtc gacaaaacct acgatttcac cccggccgcc ggccgggcca
     1501 tgctgctcag cgacctcgcc ttttcctggg gttactctcc ccccttcacc ctccgcggca
     1561 tccacagcat cagtctgggc atcaacgccc gctacatcat cggctccttt tacggtcagg
     1621 tcgaacgcat gcaggcaagg gccctcaccg gcgccaccct ggcccgggcg gagggggagg
     1681 ccaccctgcg cacggcgcag ggcggcaggg gatggggcct cgacctgggc atgacggcgg
     1741 tggtcaaccc ccatctccgc ttcggcctct tcagcgagaa tctggcgagc gggatgagct
     1801 ggaacaggga gagcgagcgc atccaggccg actttaccct gcacgccacc aacatcgacc
     1861 gcatcctgga ggaggatctc gatctggagg aggtggtgat gcacagcgac accaccctgg
     1921 cgatccccgc cttttcgctg cggctgccgc ggcgcctcca tttctccggc agctatctgc
     1981 agaaacgctg gtcgctcagt gccgaatgga gcggcggcga gacgggcgga ggggattggg
     2041 ggggagcgga agtggctctg gggggcgagt tctcgccctg gcgttttctc tggttgcgcg
     2101 ggggcgccgg gcatgcggaa gatctgggct ggagcagctc cctgggcttc ggtctgcaga
     2161 tgctcggggg gcgctgggat ttcgcctggc agggggtggg cagcctccat cccggccggg
     2221 tgcgcggcgg gctggtggcc accagcctgt ttctcattct ctagcctgat ccgggacaaa
     2281 aaagaaaaag ccgcttccat cgaagcggct ttttgcgttt gcggccggcc tgcggggcgg
     2341 gcctatttgg ccaggtactc gaagcgttcg atgaactgct tgggggtcat gcgcgcagct
     2401 gcggccaggc ggggcagcag ctccttgtcc tcgagatcgc gcttttcgag gcggaccata
     2461 tagctgcggg cgacgcggta ggagtcgctg gtgacgtcca ccaggcggac cgccgtcttt
     2521 ttagtctcct tgtcgatgat ctcctcgaag gggatgggat ggagtttgcc attgtcgaca
     2581 gagatcatgg cgttgccctt gtagatattg ttgtcattga gcaggtagcg cacagcctgg
     2641 aagccgaggt cgcgggtgta ctcctggtcg aaggggatgg gatcggcgca gcgaaggacg
     2701 tagccgatat tgatctcgac catcgacatt ttttcgccgc gggcggcgaa gcggctctcg
     2761 accttgagtt tgatgatctt gccgagttcg atttcggagt ggaggatatt gccgaacgag
     2821 tcgtattcga cgatgacgcc ggggatctgt ttgagctcct cggggctgag gcgttcggcg
     2881 acgccctcgg cgatgacgac cactccgtgg cggttgccca tggcgcggcg cttgatgacg
     2941 gcggtctcga cgatatcgca gatatggtca agggggataa agccctcatg gccgaactct
     3001 tcggcgatga tggccagggt ggcgccggcg gatttggcga tgccgagggc gagatgaccg
     3061 gcgtggcgcc ccatggcgac ggtgatgtac cagcggtcca tggtacgggc gtcttccatc
     3121 aggttcttga ccagggtggc gccgaggtcg cgggcggtct ggtaaccgaa ggtgggaaga
     3181 ttgttgggca ggggcagatc attgtcgatg gttttgggga catgggcgaa cttgatttga
     3241 ccgccggccg cgcgggcgat ctccatggcg ccgaaggcgg tgtcgtcgcc gccgatagtg
     3301 accatgtagg agacgccgag ggactggagg agtttcaccg aattctcgac cccgtttgga
     3361 gacttggccg ggttggtgcg cgaggtgcgc aggatcgatc ccccgtcgta gtgaatgcgg
     3421 gagacgtcgc tgatgcgcag ctccttgacg ttggccttga actccgcttc atcccccttg
     3481 accagccatt tgtagccatc cataatgccg tagactttga ggttatggcg ccgggcctcg
     3541 atggtcactg cggcgatgac gccgttcagt ccgggcgccg gaccgccgcc gacaaggatg
     3601 gcgagattgc cggttttcac tttctttttc gcactcatcg gagaactcct tttattggtg
     3661 gacaacactg tagagattgc gctcaacggc gggcctgttt gggccgccgg cgggggcttc
     3721 accgggaaag aggaggattg aggccttctc cctccggata ggatcccagg atcttgagaa
     3781 agctggtcat ctcttcgaga tggcgcaggg cattgcggca gcgctgctgg gcgggggtgc
     3841 cgcggaaatc gaggtaaaag atatattcga acggtcggcc tcgccagggc cgggactcga
     3901 tcttgcagag atcgatgtcg cgcagggcga aggcactcag gctcttgtaa agcgccccgg
     3961 ggatgttctt catggcatag acgatcgagg tcttgtcggc ctcggccgga accagggact
     4021 cgcggctgat gaggagaaaa cgggtatagt tctccgcgat atcctggatg ttctccctga
     4081 ggatctgcat gccggggagg gctgcggcgc gccggctggc gatggcggcg gcgtcgcgca
     4141 gattctgttc gaggatcatg cgcacgctgc cggcggtgtc gagagcgggc acggcgcgaa
     4201 tccctggcag cgaggcgatg aactggcggc actgttccag ggcctggggg tgggagtaga
     4261 tcacccgaag atcctgcagg gccgcgccgg catgggcgat gagctgatga tcgacgcggt
     4321 aatagacctc gccgacgatg gcgacctcgt gctggaggag caggtcgtaa ttgacgtgga
     4381 tgctgccggt ggatgagttt tcgacgggaa ggataccgta acgggtggtt ccggagatca
     4441 cggcatcgaa aacctgctcg aaaagggcgc agggcacgca ggaggcgttt tcgccaaaga
     4501 acttgaaagc agccagttcg ctgtaggcgt catggtcgcc ctgaaaggcg accgtggctg
     4561 gcgccgcgtc cggatcggat gccatgagtc catccatatt aataattaaa ctcaaagtta
     4621 gaatttttta gcgaaataat caagaaaatt gtacggattg ggggctggtc ggctcgcagg
     4681 atcgaggggt gggcaggtgg agggcgccat cggcgtggcg ggcggcggcg atgagggggg
     4741 cggcgatgaa aaagccgccg acgccggcgc agaggtagcc ggcgagggcc cagagcaggg
     4801 tccagccccg cgagagcggg gggcgggaga gggcgaggcg ggtggtgagg aggaggtggg
     4861 cggcgagcca ggtcactgca aagacgatgg cggctccgcc ggcctggagc gggaggtggc
     4921 ccggatcgag ggcgaagaag aggctgaggc cggcggcgaa gagggggccc cagaaggggg
     4981 tgggtgcggc cagggccatg aagagcccgg cggcggcggg gtggctgtag ccgagcagcc
     5041 aggtgccgag ggcccagagg ccgcccatta agaggctgcg caggctttcg tcgcggatcc
     5101 atctgccgaa acggtcgggc agctcgcgca agagccaggc cctccgcgtc agcgcccggt
     5161 tgggggcgag acgcagcagt tcgcggtgca ggggtgggcg cagggcggta agcaggccgg
     5221 cgaggagaag ggcaagagcc agggtggccg tcgaaggcca gagcagggca gtcagctgcc
     5281 gggcgaagcg gagcagctcg ctgcggccgg cggcgaggtg gcggctgatg agggcggcat
     5341 cgccgtgcag ggcgggggcg gcgctccccg gcggcgcgga tgcgatcagg ccggggagga
     5401 ggggtgcgaa gagctgatgg agggcgagca ggaggagggc caggagcgca cccaggcata
     5461 ggcgtctgcc cggctgcggg ggcagggtcg aggcgaggcc gttcagccag agggtgaaca
     5521 ggaggaggcg gaagaggggc ggcgcggcca gggcgcaaat aagcaggggc agcagtatac
     5581 cggccgcgat gagggcaagg gcaagaacgt ggacgctctc ttcacgggcg gggactgtcg
     5641 ggatcggcat ggcggtcttc atgagcgcgg cagggggatt cagacgatga tgttggcgcg
     5701 ggtgagatgg tcggtcaggc cgctgtaggt gcgcagcgcc tggcgccagt cgtcgagggt
     5761 ctcctcgagc agcccctgca ggagggcggc ggcgatggcg ggctggccgg cgaggagttg
     5821 gtggaaagcg gtctggggca gcaccgcgac gcggctgggt tcgagggcca ggcagctctc
     5881 accgtaaaag gcgtcgccgg cgagggcggc gctgccgcaa gcctgacccg gggcgacgat
     5941 cttgaggcgg tcgggccggc cggtccggga gtccgcctcc agggccagcg aaccggagag
     6001 cagaaaaaag aggcagaggg cgggttctcc ctggcggaag agcggctcgt cggctgcgta
     6061 ggtgcgcaca tgcagccagg tcaacaggcg tttgcggcct gcaggcgtca gacggttgag
     6121 ggcgtggagg tactcgatct cctcggggcg gcagggctgg ccggcttcct tttccggctt
     6181 tttattcgtt ctttccatag agattaatat actatacggg cggcttcaag tcaaggcctt
     6241 tgcagggcgg gtggaatcgg agcggagggg ggctgacggc caagtgaggt tcttgcaccc
     6301 ggccgcgcag gttttctgtt gcatttgtct gcatagagta ttattttcta acaagtatag
     6361 agatccctcc cgcctgcggg ctggagggat cctcctcatt ggtacaaggg atgggatgag
     6421 aagagcgcgc acagcatccg ccgtttgcct gctcgcggtt ctggcactcc cacctgcccg
     6481 ggccgagact cccagggggg tggtgcagca caagaccgag ctgcagaacc tccgggagga
     6541 gatcaagcgc caggagaaag cactgagccg gcggaagaag gatgaaaagt cggccattga
     6601 ttatttggcc aaactggacc gggagatcga tgtctcgcat gcctatctgc actccctcaa
     6661 cggggatgtg gatcgccttg gccggcaggt ggatgagcgg gacgggcgca tccgcgcctt
     6721 gacggcggag aaggaggccc tggccgaaca ggtcaaaaag cggctggtct ccttttacaa
     6781 gcacgggcgg gcgcaggaga tcgacctgat gctcagcatc cggtcggtgc agcaggccca
     6841 ggtctggatg cgctaccaga agatgatcgc cgacgccgac cgccgcaacc tccaggccct
     6901 ggccgcgcgc cggcaggagt tggagaagca gcaggattat ctccgtcagg aaaaggcgga
     6961 caaggcgcgc gccctgcggg agcgcgaggc ggaggagcgc gagctgacca gcagccggga
     7021 gaagcggcgg atttatctgg cctcggtgcg caaggacaag aaggtgctcg agcagcgtct
     7081 gcgggacagc cgcgcctctg aaaagcagat cctcgccctg atcgccaggg cggagcaggc
     7141 gcgtttgacg gcgagtgcgc gcaagccggc cgccgccaag gggcgcaccc agccgccagt
     7201 gagcagcaaa ttcgccggga tgaagggccg cctgagctgg ccggtgcgcg gcaccatcgt
     7261 cagccgcttc ggccggcagc gtcatcccga actcaacacc atcaccgaga atctcggcat
     7321 cgacatcaag acccgccagg gaggaacggt ggtgagcgtg ggcgacgggc aggtgcagac
     7381 gatcacctgg cagcgcggca gcggcaacat cgtcatcgtc agtcatggtg aaggttttta
     7441 cacggtatac acccatttgg aagagatccg cgtcagccag gatgatccgg tgcgccaggg
     7501 ccaggtgatc ggcacagtgg gcgaatcggg ttccatcaac ggtccggtgc tccatttcca
     7561 gatctggaag aacacccgca atctggaccc cgaggagtgg ctgggataag gcgcggcatc
     7621 gaccctctca ttcggcctga aagagattca tgagtttcgc acagatcatc ggccagagcc
     7681 ggccgaagga gatcctggcg cgggcgctgc agaccgggcg gatcccccat gcctatcttt
     7741 ttgccggtcc tgcaggcgtc ggcaaggagg cgatggccat tgaaatggcc aaggcgctcc
     7801 tctgcagcgg cagcgacgac aagccctgcg atggttgctc cgcctgccgc cgcgccggtc
     7861 gtttcggcca tcccgatttc ttcttccttt ttcccatgcc caagagcgcg gcggtcgagg
     7921 aggagcgcga ggtgcttgac agtctcgtcc gcgatcccta tgtgcgcaag cagccttggg
     7981 cctcgccgac gatcggcatc gagcgcattc gcgagctgcg gcgggtgagc agcctcaagc
     8041 ccctggaggg gcgccgggtt gtcatcatcg ccgaggcgga caagatgacc cccgaggcgg
     8101 ccaatgccct gctcaagatc ctcgaagagc cgccggccga catgcagatc atcctcactt
     8161 cctcgcgcga tgccgggctg ctgccgacga tcatctcgcg ctgtcagcgc gtcgatttca
     8221 acctgctcac caacgccgag gtcgcggcgg ccctggtgga gaaaaagtcc gttccggagg
     8281 agcaggcgcg gctggtggcg cgcatcgccc agggcaacta ccgtcacgcc gccgaactgc
     8341 tcgaggagaa tctgcaggaa cggcgcaatc tggtcatcga ggccctgcgc atcagcctga
     8401 aggatgaggg cacccgcatc gaatgggccg agggggtgct cgacgaactg gacaagcgtg
     8461 agctgcgcga ttttatctcc ctgatgctga tctggttccg cgacgccctg gtgctcactg
     8521 ccaaccctgc ggcggtcgcc agcgaccagc tcgtcaatgt ggatcagatc gaggtgatga
     8581 gcaaattcgt tgccgctttt agcgagatcc gtttcgaggg aattttcacc gatctggaaa
     8641 aatcgatcga gatgctcgac cgcaacgtgc atcccaccct gattctggtg gtgatgctgg
     8701 ggcggctgaa ccggaattta atactcaaag gcagtgccct atgatggtcg aaattgtctt
     8761 caagggggag cgcaaggata tctatgccaa tccccaggaa ttcccctttc aggtcggcga
     8821 ttatgccatc gtggaggcgg agaaggggga ggatctgggc atcgtcaacc agctcggatc
     8881 tgtgctcgaa cgcaaaaaaa gcgacgcggt gctgcgcaac atcctgcgca agccgacccc
     8941 gcgcgatctg gatcgctatc gccagaaccg ggtcaaggag tttgaggcct accagatctg
     9001 ccgcaagcgc atcgccgaga ccgggctcga gatgaagctg gtggacgtcg agtaccagtt
     9061 cgacaacaac aaggtgacct tttatttcac cgccgaaaag cgggtcgatt tccgcgagct
     9121 ggtgaaatac ctggccggcc ttttcaaggt gcgcatcgag ctgcgccaga tcggagtgcg
     9181 cgacgaggcc aagcgggtgg gcggctatgg cacctgcggc cgcaagctct gctgcaccac
     9241 ctttctgaaa gagttcgaac cgatcacgac ccagtgcgcc aaggagcaga acctgcccct
     9301 caatccgcaa aagctctccg ggctctgcgg ccgcctgctc tgctgcctga tgtacgagcg
     9361 cgatttttac cggcgccaga tgtcccggct gccgcgggtg gactcccgtt tccagacccc
     9421 gctcggagag gggagggtca cccacgtgga tgtctttaat gaacgcatca ccctttcctt
     9481 ctcgccggag aagcaggaga gctttgatct ggcggagatg gagaatttca ccctgatctc
     9541 ggcccctgaa gcggagtgag ggggcgcggc ttcccgagac cgccccactg cccgaacctc
     9601 gccggaccga ttgcggagcc ggctgcaccg tccatatcat ggaagagaga acaatcaaca
     9661 tgagccaaga gcagcaaaag gaatacaagc gcctgctggt gaccagcgcc ctgccctacg
     9721 ccaacgggcc gatccatctc ggccacctcg ccggggctta tctgccggcg gacgtctatg
     9781 tgcgctacca gcgcctcaaa aagcgcgatg tggtctatat ctgcggcacc gatgagcacg
     9841 gcgttcccat cgccatcgcc gctgaaaagg cgggggtgac gccccagcag atcgtcgaca
     9901 aatactgggc cgaccattac gacaccttta cccggttcgg catgagtttc gaccactttt
     9961 cgcgcacctc tgcaccgatg cagcaccgca ccgcggctga ttttttcaag gtcatccatg
    10021 acaagggctt tctggtcgag cgcgccatcg agcagttcta ctgcgaaaaa tgcgcccgtt
    10081 tcctcgccga ccgcttcgtc gagggcgagt gcccgcgctg ccaccgtccc ggggcgcgcg
    10141 gggatcagtg cgaagcgtgc ggcagttctc tcgaacagac cgagctcatt catccctact
    10201 gcaaggtctg cggaggcacc ccggtgctgc gtcagaccag gcatctcttc ctcaagctca
    10261 atgaattcca gcccctgctc gagaagtggc tggcggacaa gggggagtgg aaggagaacg
    10321 tccttaatta tgtgcgcggc tggttccagg aggggctggg ggagcgcgcg gtgacccgcg
    10381 acctgagctg gggcatcaag gtgccggtgg ccggttacga ggagaaggtg atctatgtct
    10441 ggttcgaagc cccgatcggc tatatctcgg ccaccaaaga gtgggctgag aaaaagggcc
    10501 ggcccgaggc ctggaaggag tactggcttc agcccgacac caagctggtc catttcatcg
    10561 gcaaggacaa catcgtcttt cacgccgtga tctggccggc catgctgatg gggcacggcg
    10621 gttttgtcct gccggcggac attccggcca acgagtttct caatctgcag ggtgaaaagc
    10681 tcagcaccag ccgcaactat gcggtctggc tcggggatta cctgaacaag tttccggccg
    10741 accccctgcg ttattatctt gcggtcaacg ccccggagac cagggatgcc gatttcatct
    10801 gggaggattt tcaggggcgc aacaacagcg agctggccga tattctcggc aattttatca
    10861 accgcaccct cacctttgcg cgcaagcagt ttggcggtgc cgtcccggag cgcggtgtgc
    10921 tcgacgggct ggatgagcag atggtacgga ccctggccga ggcgccggaa aaaatcggcg
    10981 ccctgctgga gggctacgag ctgcgcaagg cggccgcggc cctgatcgat gtcgcccgtt
    11041 tcgccaacaa gtatttcaac gatcaggagc cctggcgcac tgcgcgggag aaccgtgcaa
    11101 aatgcgccac gaccatccac ctcgccattc aggcctgtca gaccctggcg gtgttgatgg
    11161 agcccatcct ccccttctcc gcggagaagg tctgggccat gctcggacag ccgggcagcg
    11221 tccatgatca ggtctgggat ggctgcggca gccgccatct ggcggcgggc cacctcctcg
    11281 gtcaggagca gattcttttc accaagatcg aggagagcca gatccgtccg gaggtggagc
    11341 ggctgcgcgc gatctcggag gcgctcaagg ctgcgtcggc cggcgctgag gttccggcgg
    11401 agaaagcgga ttcagaggcc ccggcaaggg ccgccgccgt tggtgcgggc caacccgctg
    11461 ctgctgccgg tccggcagct caagggaacc cctctgccgc tgccgcagaa aaaccgcgca
    11521 tcacctatga cgagttcgcc aaagtggatc tgcgcgtcgc cagggtcgtc aaggcggaaa
    11581 aggtcgaaaa ggccgacaag ctgctgcgcc tcgagctgga ggtggggagc gagatgcggc
    11641 agatcgtcgc cggggtggcc aaacattaca ctccggagga gatgaccggc cgcaccatca
    11701 tcatcgtcgc caacctgcag ccggcggtga tccgcggcat cgagtccaac ggcatgctgc
    11761 tcgccgccga ggacgggcag ggtcggatgg cgatcctgtc accgcagggg gagatcgatt
    11821 cgggcgcgaa agtgaaatga atccctggct gatcgacacc catgcccacc ttgatttcga
    11881 ccagttcgac gccgaccgcg aggagatgct cgagcgcgcc tggatgaacg gggtgcagca
    11941 gatcatcacc atcggcatcg atctggccac cagccgcaag agcgtggcgc tggcggaaaa
    12001 acatgccatg gtctatgctt ccgtgggcat ccatccccat gacgtcgcca gggctggtgc
    12061 ggaggatctg gcggagatcg aatccctgct cgagcatccc aaagtggtcg ccctcggcga
    12121 ggtcgggctc gatttctatt acaattactc gcccgaagag gtgcagcgca aatatctgcg
    12181 cctctttctc gattggtctc tggagaaaga gaggcctttg atcattcaca cccgggatgc
    12241 ggacgaagcg atcctgtcga ttctgcgcga gcgcagccgg gccggatggc agggggtttt
    12301 tcactgcttc tccggcgacg cgcgcatggc ggaaaaggtg ctggagatgg gctttcacat
    12361 ctccttcacc ggcaacatca ccttcaagaa ctcgacatcg gccggggtga tgcgcggggt
    12421 gccgctggag cggcttatgg tcgagaccga ctgtcccttc atggctccgg tcccgcatcg
    12481 gggcaagcgc aacgagcccg cctatgttca actggtagcg cagaaaatcg ccgaagcgaa
    12541 gggtattgcg ttcgaccagg tggctgagcg gacgacggcc aatgcccggg cgctcttcaa
    12601 tctgcccggg gtatgacagc tgcccagggt tcgcgacgga tactgaggct ttccaaaaat
    12661 ggagaaggat gagtatgaaa agaggaaatg tctgcctgtt cgctgccggt ttgtggaccg
    12721 ccctcctctt caacatgcct ggagagggga atgcgcagca aaaaatgcct ccgcaagatt
    12781 tctttgaatt cagcaccagc tattttgatg agggtgaaga ccatgtcgcc atctacatcg
    12841 gcggctttcg taaaaaagag ttcctctatg gctaccatga tgcttggggc acctacgtta
    12901 cagagtataa agagaccaaa attgaccggt ctgatgagat ctggaaatat aactttcgca
    12961 cccaggaatg ggtgcagctc aagccttatg actcctcctg gctcaaggtt ccgcccatgg
    13021 ccgggcatca ggccctgcag aacaagcggg aaggatacat ctactgtttc ggcggccgca
    13081 gcgccaacgg cgacagcgcc gtctacatgc tcaacatgca aaaacgagag tggcggcagc
    13141 tcgcatttca taaattctat gacaaggaaa aaatagccgc tgccgcccat ttcacgcctt
    13201 tcaccgatgg cggtaaacaa tttggcggca gggcggtatt ccacggcgga gtcgacaaca
    13261 acggctctgt ttccagcatg ctctaccagg cggatttcaa cggcgaagag atccagttca
    13321 cggagaaaca gacgccgcgc gctctttatg gccattcggc catctatgat ccgggcgccg
    13381 agacctggca tttttggggc ggccagtacg agggtggcat caattacgaa aattaccagt
    13441 acaacccgaa gagcggtttc ggctgggggc cgcctgtgca agggcccttt gtcatgccca
    13501 gggcatccgc cgcgactctt ctcgacgggt ccaccctcta cgcctggggc ggcaagggct
    13561 acaccttcct gcgcaagggc ggtgctgcgg tcggtgagga gatccgcgat gacctgatct
    13621 cgatcacggc gcgcgacggc cagctttaca gccggatcgt cgccaccggg ctgccaccag
    13681 cctcgcaagc atctctgtcc atggagatcg ttgacgggga taccctgttc tatcttttcg
    13741 gcgggatttc gacgatctgg agcagcggcg atacctccgt gaacaacacg gggtaccggt
    13801 acagccttac ccaaaaaagt gtgcagcagt tcgacaccac caccgcgcag tggggccgcc
    13861 tgcagaccag cgtcgaggcg gagcgttttc cggtctctcc cgaactgcgc ctggaggtgc
    13921 cggtctggcc caatccggcc ggccgcacag tctccttcgc cctcgccggc ggaagtgcaa
    13981 tccgctcggt caaactttac aatgaactcg gccaacttgt gctgcagatc catcatcccc
    14041 gcgagcagcg ccttgatctg tcgcgctgtc cggccgggct ctatttcctg cgcgtcgata
    14101 cggagtccag accctatttt gccaagatcg tcaagaaata agcggccggg cggccgcgta
    14161 tgagcggaga gctgctcctc ctgactgcgg cggtgatcgt ggtcgtcagc gtcctgctga
    14221 gcaaggtctc ggagcgtttc ggcgtgccga ccctgctgct tttcctcggc atcggcatgg
    14281 tggccggcta tgatggcccc ggcggcatct atttcgacaa cgcccgcatg gcgcagaccc
    14341 ttggcacgat agcgctggcc tatatcctct ttgccggcgg tctggagacc gatgtcgctg
    14401 cggtacgccg ggtggtgcgg ccggcggtgg ctctggccac gttcggggtt ttttcgaccg
    14461 cccttatcac cggcctcggc atccattacc tgctaggcca caactggatg gagagtctgc
    14521 tgatcggggc gatcgtctcc tcgaccgacg cagcagcggt tttcgctgtg ctgcgttcaa
    14581 aaaatgtcgg gctcaaggag ccgctccggc ccctgctcga gctcgagtcg gggagcaacg
    14641 atccgatggc ggtctttttg acgatcggaa tgatcgcgct catccgtgaa cccgcccggc
    14701 cggtgctgag cctgatcccc ctttttctcc agcagctgat catcggcctc gccctgggct
    14761 atctcatggc ccggttcttc atacgcatca tcaaccgggt caagctcgac tatgaggggc
    14821 tctatccggt cctctccctg ggctgggtgc tgctgaccta tgccctgacc gcctggctga
    14881 aggggagcgg atttctggcg gtctatatca ccggactggt gctgggcaag gagagttttt
    14941 cgcacaagaa gagcctgatc cgtttccagg atggcatcgc ctggctgatg cagatcctga
    15001 tgtttctcac cctgggactg ctggtttttc cctcgcagct cattccggtg gccggccagg
    15061 ggctgctcgt ggccctgctg ctgatcctgg tggcgcggcc agccgcggtc tttatcagcc
    15121 tgctcccctt tcgtttcagc ttccgagaaa aacatctgat ctcgtgggtg ggattgcgcg
    15181 gttcggtgcc gatcgtgctg gccacctttc ccatgctggc ggggctggat cagacccatt
    15241 tcatcttcaa tctggttttc ttcatcgtca tctcctccgt cctgctgcag ggcaccacca
    15301 tccccctgat ggcgcggctg gcccgggtcg atgcccccct ggtgcgccag cgccgctacc
    15361 cgatcgattt cgacaatccg gaggggatgg acctgcagct ggtggacctc tttgtccctt
    15421 acggctcgga ggcggcgggg cagcaaatcc tcgaactcgg ttttcccagg gactgcctga
    15481 tcaccctgat ctcgcgcaac gagaaattca tcatgccggg gggatcgacc gtgctcgagg
    15541 agggggatgt gctgctcatg ctctccagtc ctgagaatct ggcggtcgcg cgcgagatcc
    15601 tcgagaagcg cagggaggag aagccgcccg gcgggtgaga agtgcagaga agggttgcat
    15661 cagagcaaaa cgattggtaa attgatgcca ttgtgtatcg gtggaattat tggagaatgg
    15721 atgccatgga atatctgacc ggcagggccg gccgcgacga aaagagcgac cttgtggtcg
    15781 agatcgagat gcgccgggag ggcggcctgg agattcaggt gaacagctcg gtcaaggccc
    15841 tattcggcaa ggcgatcaca gccaccgctg cagggacatt gcaggaactg ggcatcggcc
    15901 atgcccgggt gacgatcaac gacaaggggg cgctggactg ggtgatccgg gcgcggatcg
    15961 aggcggccgc ccgcgccgcc ggctacaagt cggggctcaa gccccttgcc gtggcgccgc
    16021 acccgccggc tgacaggaac cgcctgcggc ggacacgcct ctacctgccc ggcagcaatc
    16081 ccgatctcat gctcaacgcc catctcttcg gggcggactg tctcattctc gacctcgagg
    16141 actcggtggc cccggtggaa aagttcgcga cgcgcatcct ggtcaagcat gcccttgcgg
    16201 cccttgaatt cggcagcagc gagcgcattg tgcgcatcaa tcccctgcag accccctac
//
LOCUS       DAOPWU010000034        15741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3805_length_15741_cov_4.481193, whole genome shotgun sequence.
ACCESSION   DAOPWU010000034 DAOPWU010000000
VERSION     DAOPWU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15741
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3805_length_15741_cov_4.481193"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKM23_03425"
     CDS             <1..402
                     /locus_tag="PKM23_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNY90556.1"
                     /translation="NKLVLPAGENNLIITFHYYNPFHFTHQGASWASGSDAWLGTTWG
                     SAADMTTLQNELEVARNWARTNNRPLFMGEFGAFNKADMNSRKLWTEAVARQAENRAM
                     SWGYWEFGSGFAIYNLSKNEWYLPLLKALIP"
     gene            475..1821
                     /locus_tag="PKM23_03430"
     CDS             475..1821
                     /locus_tag="PKM23_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNY90557.1"
                     /translation="MDVFLLSRLQFAFTIAFHYIFPPLSIGLGVLLVIMEARWLKTRD
                     PLYLEMTKFWARIFGLIFALGVASGIVMEFQFGTNWSIYSRYVGDVFGSALAAEGIFA
                     FFLESGFLAILLFGWNRVKPVTHFLATCMVALGAHFSAVWIIVANSWQQTPAGHHIVG
                     FGTGLRAEIVDFWQVVFNPSSLHRLSHTLAGAWQAGAFLALSVAAYYILRNKHIAFSK
                     ATLRIALPVAMIASLLQLLTGHASASGVARNQPAKLAAFEGHYPASAPADMYLFGWVN
                     QEKERVQLGLRIPGMLSWMVHGDSKAPLTGLQAFPPDDRPPVNLVFQTYHLMVLLGMG
                     LIGLSLLALLLQFRNRLSEQRWLLWIFVFAVLGPQAANQLGWASAEVGRQPWIVQNLL
                     RTRDAFSPNVSGGELLFSLVMFFIVYALLLALFIYLLNDKIQHGPEERTSMEGHRA"
     gene            1818..2846
                     /gene="cydB"
                     /locus_tag="PKM23_03435"
     CDS             1818..2846
                     /gene="cydB"
                     /locus_tag="PKM23_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNY90558.1"
                     /translation="MNLAFDLNTIWFFLVGVLFAGYAMLDGFDLGVGALHLFTRGDEN
                     RRILLNAIAPIWDGNEVWLVTGGGALFAAFPEVYATAFSGFYVAFMLLLFGLIFRAVA
                     IEFRSKQPMAWWRRFWDISFSLSSILSSLLIGVALGNMAWGVPLDAQGNFNGSFWSLI
                     HPYSVLVGLCTVALFTMHGSIYLVLKTEGALQQQVREWSRSTIISFVLFYITVTMFTL
                     LYVPHMTDTIRANPWLFLVALVNMLAIANIPREIFHGREMRAFLSSCAAVIALLLLFG
                     IGTYPNLVYSNPQPANSLTIYNAASSRKTLQIMLIIALCGVPMVLAYTGSIYWIFRGK
                     VKLDAHSY"
     gene            2978..3544
                     /locus_tag="PKM23_03440"
     CDS             2978..3544
                     /locus_tag="PKM23_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90559.1"
                     /translation="MTRILLITLLFIGAAMAQEPPVTTAVVPATAAASDSLNPHLLPF
                     KPYLNRTWKGTFAQEPGQALVTDISRWERILNGQAIRMTHSVDEGVYGGETILFWDQA
                     RETLVYYYFTTAGFFTHGTMSFNGDKWVAHEMVENNANGITEVRSTSEFLPDGTMQSV
                     AEFLQNGQWVHGHTIRYVESPGSPLIFK"
     gene            complement(3648..4142)
                     /locus_tag="PKM23_03445"
     CDS             complement(3648..4142)
                     /locus_tag="PKM23_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNY90560.1"
                     /translation="MGTKGREIVGMNVDHVIELLNKAFADEWLAYYQYWLGAKVIAGP
                     MKDAVIAELIQHAADELRHADMVANRIIQLGGTPLTTPQQWFKMSTCGYDEPADPFVK
                     MILGQNIKGEQCAISVYNALLQEVGMKDPVTYNMAVQILQDEVMHEEDLQALLEDLNF
                     MLKK"
     gene            complement(4209..6863)
                     /locus_tag="PKM23_03450"
     CDS             complement(4209..6863)
                     /locus_tag="PKM23_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNY90561.1"
                     /translation="MNYPIWELTWFGGSSMIALIAVLHAYISHLAVGGGIFIWLMDWK
                     GFRENNPEIHVYVRRYTWFFLLLTMVFGGITGVGIWFVIALVQPAATSALIHSFVFGW
                     AIEWVFFLGEITALLIYHYNFSKLDKRPRLNIAFLYALFAWLSLVVINGILAFMLTPG
                     KWLQTGSFWHGFLNPTYFASTCFRTGVAVMIAGLFGIFTAVRIPNGFSRDQILRYCSK
                     WLLLALPIMAATGYWYYMSIPAATREVAFGLNPQSEPFILIALAAAAAIFLLALYYLL
                     RSPRGLQQVLAVLLVLAGLGFYGGFEYLREIARKPWVIHGYMYSTSIHAADGARLNEE
                     GVLPNARWTQHKEITPDNQLQAGKDLFDIQCLPCHTVGGVRNDILSRTGMFPQEGMLA
                     FLHGQGRINSYMPPVAGTPAEREALAAWIVQGLHGKAPEAATTTASAPAARKAEKIPP
                     FDSRKDDYLLLAWNDLGMHCMTDCDAWFVILPPANTLHAQLIRRGALPALISDGVTLS
                     YKVEEGFENPAGQMPFWKHVKANFGVDLPENIGLAGKGLSGEMEYKADFASFAAEMIP
                     VGPYSDDGAFNAFPTFTVEAREKVGGRLLASTRVVAPVSSEMGCRTCHGGTWKRGVAG
                     VSDETAVNILAAHDRNSGTTLLEQAKQGKPALCQSCHADPAVGAKGDSLRLNFSAAMH
                     GWHANYMHLEGAAACAACHPARPDGATRCARDFHKEIGITCSECHGSLEEHAMALLRG
                     EEAKPGAARLVRHLKSDQVASASEVHPRTPWLQEPDCLNCHAGFNIPEDIVSFNKWTP
                     GFTGLFRVRTDNAGIRCEACHSATHALYPADNPFVLWRDNIQPRQYTGSSYAIGANKN
                     CAVCHKKVMTDPVHHENMLGMVRNPFDF"
     gene            complement(6860..7921)
                     /locus_tag="PKM23_03455"
     CDS             complement(6860..7921)
                     /locus_tag="PKM23_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90562.1"
                     /translation="MDGIPLIPTLEAIPAPWPVFEVLGVLLFMLHILFINVVLGGSLL
                     AFFSKAPRENGAASAIYKKIPSTLAIGVTLGVAPLLFLQVLYGHLIYSSSVLMALYWL
                     LVIPLLIIAYYGTYIHARSAVNWLGRTAIGLAALIFLYITFIYSNNMTLMLQPEKWSA
                     YFTNRSGTILNLSDPALWPRYLHFVIASVAVAGLFSSLVWQIRARKGVPGSGEEVRNG
                     LRIFAIATSVQVAVGIWFLIALPRDFMLAFMGGNLLYTLILIVGIILAIGALVLAFLG
                     KFWPTFWHLIALTLVMVLNRLALRDLYIGRFFKVSELTLKPQYDLLILFVIILLIGLA
                     AVWYMVRTMISAGEGRESE"
     gene            complement(8067..8981)
                     /locus_tag="PKM23_03460"
     CDS             complement(8067..8981)
                     /locus_tag="PKM23_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90563.1"
                     /translation="MSRIPELRHLLLFFIDGLGIGADDPEINPCCHPSLNLFRQCHTG
                     IFPRPTASEGLAMGLDANLGVEGLPQSATGQTSLLTGVNGARLLGRHLNAFPNKALRQ
                     VIAEHNILKRFTDHGFRAAFLNTFRPPFFDLDPFQIINRLSVTTVSNLYAGLRFFDLD
                     DLRARRSVYHDITGAGLIDHGFDVPLYTAQEAGEIIGAASREYDFSLFEFFQSDVAGH
                     SRDFARALGVLTRLEDFLAGILAVVDLDHTLVLVTSDHGNIEDLAVKGHTRNPVMTLL
                     FGCGREEIASRLHSLTDVAPALLRALAG"
     gene            complement(8978..9373)
                     /locus_tag="PKM23_03465"
     CDS             complement(8978..9373)
                     /locus_tag="PKM23_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNY90564.1"
                     /translation="MLTGSEPSLWLYILLGLAAGTLSGFFGIGGGIIIVPALVYFAGF
                     SQQMATGTSLAVLLPPVGLAAVLVYYQNNNVNLKAAVIIAICLFIGAWAASHIANRLN
                     QLHLRLAFGVVMVLLGLFMVFNSWAKLHK"
     gene            complement(9438..11054)
                     /locus_tag="PKM23_03470"
     CDS             complement(9438..11054)
                     /locus_tag="PKM23_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNY90565.1"
                     /translation="MKTLCKLLAGAVLLVAAGCTQMRYVPVAGDPIIRVGILEDQEAV
                     YFEPAGEFSVVCYDEKENFEALEKGLWKATVHTLVPGALTYQIQLYESLDSDRAAERA
                     KALRAKGLAVTVKSQGEQLKAGTKNLIDRIYHRVLLDRSFATTAEAERHLAATPALAQ
                     GKVVRSMDDVVMGKIALQTPAGRTIILTQALRLTGANITLRDVEVGSGYHWARNEART
                     YGGEMELRINAKGRLTAINVVPLELYLRGVVAGEMANSFPEEALKAQAIISRTMFLNS
                     FGRFHRGAEFDVCDDVHCQAYIGIGKNSDPVEKAVHDTKGLVLTYNDALCTASYSAMC
                     GGHTASAAEVWDSDGEPYLTGRLDTPTEIGRFDLTREENVRVWVNSRPEVCCNLEAAG
                     NPDFGRYAEKYFRWEQRIARTDLEKSIADYAGASIGQLVDIIPLERGTSGRLISINCI
                     GTEESVKIHKELNIRRALSKSTLYSACFTIEKEGVKDGLAESFIFRGAGWGHGVGLCQ
                     LGAAVRAKRGDSVSAILKHYYPGTQIKQLY"
     gene            complement(11065..11994)
                     /gene="era"
                     /locus_tag="PKM23_03475"
     CDS             complement(11065..11994)
                     /gene="era"
                     /locus_tag="PKM23_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNY90566.1"
                     /translation="MSTTREQESRKGAEPFRSGYVAVAGQPNVGKSTIVNRLLQFKLS
                     ITTPKPQTTRHRIMGILTGESYQIIFLDTPGLITPSYRLQEVMMETARRAVLDSDLLL
                     FMVAAAARPEERDREILAELAAARKPLLLGINKIDLVERGLLPALEQAWSALHPFTGI
                     VPFSAHTGENCDLLLDTLTARLPAGVPFYDQDALTEHPERFFVSEIIREKIFAHFGEE
                     IPYSTAVIIDEFREGRPDRKDLIKARIVVERQSQKGIIIGRGGAMLRRVGEEARADIE
                     AFLDRPVFLELWVAVRDKWRKNDTFLREFGYQG"
     gene            complement(11991..12443)
                     /locus_tag="PKM23_03480"
     CDS             complement(11991..12443)
                     /locus_tag="PKM23_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90567.1"
                     /translation="MHKNKDVVELEVPSKVLRIQNASCPNGHSLMDENHLINGYPSVS
                     VLARYHDETGLIHLDPIYGSFKNISQITVPDGELVEFLCPTCSVSLQDADQRCSVCSA
                     PMFAMQLPKGGIVEGCLRNGCQFHSLKLVSSDELVKRLYESYSLDSYL"
     gene            complement(12506..13936)
                     /gene="proS"
                     /locus_tag="PKM23_03485"
     CDS             complement(12506..13936)
                     /gene="proS"
                     /locus_tag="PKM23_03485"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNY90568.1"
                     /translation="MAKEITTRAADYSQWYLDVVAAADLAEHAAVKGCMVIKPTGYAI
                     WEMIQGHLDKMFKATGHVNAYFPLFIPESFMKKEAEHVEGFAPECAVVTHGGGKKLDE
                     PLVVRPTSETIIWSAYKNWIQSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGH
                     TAHATAEEAEEETLKILDVYRRFAEEYMAAPVFYGVKTESQRFAGALRTYCIEAMMQD
                     RKALQAGTSHNLGQNFARAFDVTFQNEQGKLELVYATSWGVSTRLIGALIMTHSDDKG
                     LVLPPRLAPIKAVLVPIWKEDSEREMMLAKARALTSGWEESISFKIDDRDQYRPGYKF
                     NEWEKKGVPLRIEMGPKDVEANQVVLVRRDTGEKIAVPQENLLARIEALLEEMQHNLF
                     VRARAFRDANTFHVDRYAELAEQIEKPGGFFWVHWCGSPDCEARFQEEHKATIRLVPI
                     DEEKEEGACIVCGGRSGQRVLVAKSY"
     gene            complement(13960..14847)
                     /locus_tag="PKM23_03490"
     CDS             complement(13960..14847)
                     /locus_tag="PKM23_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90569.1"
                     /translation="MKNPYKRTDVFTCHYARHAAFAHRVSAWHVLGARKCYPQGCLEF
                     RWSCALLNKGKRCSRGYNRMGRLCPGCAHYRDEKIHYQPRITLSPAEYALFQQDLAAF
                     DEWVEENQGREREISFRIDSLKPRFHKQIDVHGARLHLHGYLLIAREGFIGLDAFEDY
                     FYAAISPQQQERLALAPGDEMDARAAFTIDQGRLLFPRLRAIEVMSRSGVETWHNSSA
                     LVARQSALALPRQPDKCLHCPQGALVDVIDRSNGQERRRRELLCLEGMASAEACYFQV
                     RQQLAAEPAGCTSRPGSNH"
     gene            15224..>15741
                     /locus_tag="PKM23_03495"
     CDS             15224..>15741
                     /locus_tag="PKM23_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNY90570.1"
                     /translation="MNFSPLDFAIIFAYLAGVTALGAWFGKKQSSTTDYFLGGRDLPW
                     LAICISVVATETSVLTFISIPGLAYLTNLNFLQIAAGYLLGRIVISFVLLPRYYRGEI
                     QTAYHLLGNRFGSRMRSFSSIVFQLTRLLADGVRLFAASIPLHVITGWDYSTSIIVIA
                     MVTIIYTFIGGIR"
BASE COUNT         3064 a         4750 c         4848 g         3079 t
ORIGIN      
        1 aacaagctgg tgctgccagc cggtgagaac aacctcatca tcacctttca ttactacaac
       61 cccttccact tcacccacca gggagcgagc tgggcctccg gctccgatgc ctggctgggg
      121 acaacctggg gatctgcggc cgacatgacc accctgcaaa acgagctgga ggtggcgcgg
      181 aactgggcgc gcaccaacaa ccggcccctc ttcatggggg aattcggcgc ctttaacaag
      241 gcggacatga acagccgcaa actctggacc gaggcagtgg cgcggcaggc ggagaatcgc
      301 gccatgagct ggggctattg ggagttcggt tcgggcttcg ccatctacaa cctcagcaaa
      361 aatgaatggt acctgccgct gctcaaggcc ctcatcccct gacgggcggc gccccacagg
      421 accggcagca gcgggctgac cgcaaagccg ctaacccaac acgggagtga ccccatggat
      481 gttttcctgc tctcgcgact gcagttcgcc ttcactattg cctttcacta catctttccg
      541 ccgctgagca ttggcctggg cgtgctgctg gtgatcatgg aggcgcgctg gcttaagacc
      601 agagatccgc tctatctgga gatgaccaaa ttctgggcgc gtattttcgg gctcattttc
      661 gccctggggg ttgcttccgg aatcgtcatg gagtttcagt tcggcaccaa ctggtcgatc
      721 tactcccgct acgtcgggga cgtcttcggc agcgccctgg ccgcagaggg gatctttgcc
      781 ttctttcttg aatcgggctt tctcgccatc ctgctcttcg gctggaaccg ggtcaagccc
      841 gtcacccatt tcctcgccac ctgcatggtc gcgctggggg cccatttcag tgctgtgtgg
      901 atcatcgtcg ccaactcctg gcagcagacc ccggcggggc accatatcgt cggcttcggc
      961 actggcctgc gcgccgagat tgtcgatttc tggcaggtgg tcttcaatcc ctcctccctg
     1021 caccgcctca gccacaccct cgcgggagcc tggcaggccg gggcttttct cgccctcagt
     1081 gtcgccgcct attatatact gcgtaataaa cacatcgcct tcagcaaggc caccctcagg
     1141 attgccctgc ccgtggccat gatcgcctca ctgctgcagc ttctgaccgg ccacgccagc
     1201 gccagcggcg tcgcccgcaa ccagccggcc aaactggccg ccttcgaggg acactatccc
     1261 gccagcgccc cggccgacat gtacctcttc ggctgggtca atcaggagaa ggagcgggtg
     1321 cagctcggtc tcaggatccc cggcatgctg agctggatgg tccatggcga cagcaaagcc
     1381 ccgctgactg gcctgcaggc cttcccgccg gacgatcgcc caccggtcaa tctggtcttc
     1441 caaacctacc atctcatggt cctgctcggc atgggtctga tcggactctc cctgctcgcg
     1501 ctgctgctcc agttccggaa tcgtctcagt gaacagcgct ggctgctctg gatcttcgtt
     1561 ttcgctgtgc ttgggccgca agcggccaac cagctcggct gggcctccgc cgaggtcggg
     1621 cgccagccct ggatcgtgca gaacctgctc aggactcgcg acgccttttc gcccaacgtt
     1681 tcgggaggcg agcttctctt ctccctggtg atgtttttca tcgtttacgc gctgcttctg
     1741 gccctgttca tctacctgct caatgataaa atccagcatg ggcccgaaga acgcaccagc
     1801 atggagggac accgcgcatg aacctggcct tcgatctcaa taccatctgg tttttcctgg
     1861 tgggtgttct cttcgccggc tatgccatgc tcgacggctt tgatcttggc gtcggcgccc
     1921 tccacctctt cacccgcggc gacgagaacc gccgcatcct gctcaacgcc atcgccccga
     1981 tctgggacgg caacgaggtc tggctggtca ccggcggggg cgcgcttttc gccgccttcc
     2041 ccgaggtcta cgcgaccgct ttctccggtt tttacgtcgc ctttatgctt ctgctcttcg
     2101 ggctgatttt ccgggcggtg gctatcgagt tccgcagcaa gcagccgatg gcctggtggc
     2161 ggcgcttttg ggatatcagc ttcagcctca gcagcatcct ctcgagcctg ctcatcgggg
     2221 tggccctcgg caacatggcc tggggtgtcc ccctcgacgc ccagggcaat ttcaacggct
     2281 ctttctggag cctgattcat ccctacagcg tgctggtcgg cctctgcacc gtcgccctct
     2341 tcaccatgca cggctcgatc tacctggtgt tgaaaaccga aggggcgctg cagcagcagg
     2401 tccgcgagtg gagccggtcg accatcatct cctttgtcct tttttatatc accgtcacca
     2461 tgttcaccct gctctacgtg ccgcacatga ccgataccat ccgcgccaat ccctggctct
     2521 tcctggtcgc actggtcaac atgctcgcca tcgccaatat cccgcgcgag atttttcacg
     2581 gccgtgagat gcgcgccttt ctgtcgagct gcgcggcggt gatcgccctg ctcctgctct
     2641 tcggcatcgg cacctatccg aacctggtct attccaaccc ccaacccgcc aacagcctga
     2701 ctatctacaa cgccgcctcc tcgcgaaaga ccctgcagat catgctcatc atcgccctct
     2761 gcggggtgcc aatggtgctc gcctataccg gcagcatcta ctggatcttc cgcggcaagg
     2821 tcaaactcga cgcacacagt tattagagga gtggggtgca gaatgcagca ggatgaggcg
     2881 gacgcccgcc atcgaccctg ccgggacgaa taaatgcgag atcgtttgtg tttaagcaat
     2941 cacacgccac aaaccacctt gctttttgga gcataacatg accagaatcc tgctcatcac
     3001 ccttttgttc atcggggccg ccatggccca ggagccgccc gtaacgacag cggttgttcc
     3061 ggcgacggcc gccgcgtccg acagcctcaa cccccatctc ctccccttca agccctattt
     3121 gaaccggacc tggaagggca ccttcgcaca ggagcccggc caggccctgg tcaccgacat
     3181 ctcccgttgg gagcgcatac ttaacggcca ggccatccgc atgacccact cggtggatga
     3241 gggcgtctat ggcggcgaga ccatcctttt ctgggatcag gccagggaga ccctggtcta
     3301 ttattacttc accaccgccg gctttttcac ccatggcacc atgagcttta acggggataa
     3361 atgggtggcc catgagatgg tcgagaacaa cgccaacggc atcaccgagg tgcgcagcac
     3421 cagcgaattt ctgcccgacg gcaccatgca gtccgtcgcc gagtttctgc agaacggtca
     3481 atgggtgcac ggccacacca tccgctacgt cgaatccccc ggcagccccc tgatcttcaa
     3541 ataggccgcc tggcagcgtt tgatccagga cgattcgcct gtaataaaaa aaggcctccc
     3601 cagcgggagg cctttttttt ggcagcgttt caaaaagaag ggcggatcta cttcttgagc
     3661 atgaaattca gatcctcgag cagggcctgc agatcctcct cgtgcatcac ttcatcctgg
     3721 aggatttgca ccgccatgtt gtaggtgacc ggatccttca tgccgacctc ctggagcaag
     3781 gcgttgtaga cagagatggc gcactgctcg cccttgatgt tctggccgag gatcatcttg
     3841 acaaagggat cggcgggttc atcatagccg caggtgctca ttttgaacca ctgctgcggc
     3901 gtcgtcaggg gggtgccgcc gagctggatg atgcggttgg cgaccatatc cgcatggcgc
     3961 agttcgtcgg cagcgtgctg gatcagctca gcaatcaccg catccttcat cggtccggcg
     4021 atgaccttgg ccccaagcca gtactgatag taggccagcc actcatcggc gaaggccttg
     4081 ttgagcagct cgatgacatg gtcgacattc atgccgacga tttcgcgtcc ttttgttccc
     4141 ataacgtctc cttggttgca tgggcccgcg caggacgagg cccggttttg ttcactgccc
     4201 gctttggatt aaaaatcaaa gggattccgg accatgccta gcatgttctc gtggtgaacc
     4261 ggatcggtca tcactttttt atggcagacc gcacagttct tgttggcgcc gatggcatag
     4321 gagctccccg tgtactgccg cggctggata ttgtcacgcc agagcacgaa ggggttatcg
     4381 gccggataga gggcgtgggt ggcactgtga caggcctcgc agcggatgcc ggcgttgtcg
     4441 gtgcgcacgc ggaagaggcc ggtgaatccg ggcgtccatt tgttgaaact gacgatatcc
     4501 tccgggatgt taaaaccggc atggcagttg aggcagtcgg gttcctgcag ccagggggtg
     4561 cgtggatgga cctcgctggc gctggcgacc tggtccgatt tcagatggcg gaccagccgt
     4621 gctgccccgg gcttggcctc ttcgccgcgc agcagggcca tggcatgctc ctcgaggctg
     4681 ccgtggcact cgctgcaggt gatgccgatc tccttgtgaa aatcgcgtgc gcagcgggtg
     4741 gcgccgtcgg gcctggccgg atggcaggcg gcacaggcgg ccgctccctc cagatgcata
     4801 taattggcat gccagccatg catggcggcc gagaaattga ggcggaggct gtcgcctttg
     4861 gcgccgaccg ccggatcggc gtggcagctc tggcagaggg ccggcttgcc ctgttttgcc
     4921 tgttccaaaa gggtggtgcc gctgttgcgg tcgtgggcgg caaggatgtt gacagcggtc
     4981 tcgtcgctca ccccggcgac gccccgcttc caggtcccgc cgtggcaggt gcggcagccc
     5041 atctccgatg agaccggcgc caccacccgg gtggaggcga gcaggcggcc gccgactttc
     5101 tccctggctt ccaccgtgaa ggtggggaag gcgttgaagg cgccgtcgtc gctgtagggg
     5161 ccgaccggga tcatctcggc ggcaaagctg gcgaaatcgg ccttgtactc catctcgccc
     5221 gatagcccct tgcccgccag gccgatattc tccggcaggt cgacgccgaa attggccttg
     5281 acatgtttcc agaaaggcat ctggccggcc ggattctcaa agccctcctc caccttgtag
     5341 ctcagggtca ccccgtctga tatcagggcc ggaagggcgc cgcggcggat gagctgggca
     5401 tgcagggtgt tggccggggg caggatgaca aaccaggcgt cgcagtcggt catgcagtgc
     5461 atccccaggt cattccaggc cagcaagaga tagtcatcct tgcggctgtc gaagggggga
     5521 atcttttcgg ctttacgggc agcaggagcg ctggcggtgg tggtggccgc ctcaggggcc
     5581 ttgccatgga gcccctgcac gatccaggcc gccagggcct cccgttctgc gggagtcccg
     5641 gccaccggag gcatgtagct gttgatccgg ccctggccat ggagaaaggc aagcatcccc
     5701 tcctgcggaa acataccagt gcggctgagg atgtcgttgc gcaccccgcc gacggtatgg
     5761 cagggcaggc attggatatc gaagagatcc ttgcccgcct gcagctggtt gtcgggtgtg
     5821 atctccttgt gctgcgtcca gcgggcattc ggcagcaccc cctcctcatt gaggcgggcg
     5881 ccatccgcgg catggatcga ggtgctgtac atgtagccgt ggataaccca gggcttgcgg
     5941 gcgatctcgc gcaggtactc gaacccgccg taaaagccca gtcccgccag caccagaagc
     6001 acggccagca cctgctgcaa cccgcgcggg gatctgagca gatagtagag ggcgaggagg
     6061 aaaatcgccg ctgcagccgc cagggcgatc agaatgaagg gctcgctctg gggattgagc
     6121 ccaaaggcca cctcccgtgt tgcggcggga atgctcatgt aataccagta gccggtcgcc
     6181 gccatgattg gaagggccaa cagcagccac ttgctgcagt agcgcagaat ctggtcgcgg
     6241 gaaaaaccgt tgggtatgcg cactgctgta aaaatgccga aaagtcccgc gatcatcacc
     6301 gccaccccgg tgcggaagca ggtggaggcg aagtaggtgg gattgaggaa gccatgccag
     6361 aagctgccgg tctgcagcca tttgcccggg gtgagcataa aggcaaggat gccgttgatg
     6421 acgaccaggc tgagccaggc gaagagggca tagagaaagg cgatattgag gcgaggcctc
     6481 ttgtccagtt tgctgaaatt gtaatgatag atgagcaggg cggtgatctc gccgaggaag
     6541 aaaacccact cgatcgccca gccgaagacg aaggaatgga tcagggcgga ggtggccgca
     6601 ggctggacca gggcgatgac gaaccagatg cccacgccgg taatgccgcc gaagaccatg
     6661 gtcagaagca ggaaaaacca ggtgtaccgg cgcacataga cgtggatctc gggattgttc
     6721 tcgcggaatc ccttccagtc catcagccag atgaagatgc cgccgccgac ggcgagatgg
     6781 gaaatatagg catgcagcac ggcgatgagg gcgatcatcg aactcccgcc gaaccaggtc
     6841 agctcccaga ttggatagtt cattcgcttt ccctcccctc acccgcagag atcatcgtgc
     6901 gcaccatata ccataccgcc gccagaccga tgagaaggat gatgacgaag aggatgagca
     6961 agtcatactg cggcttgaga gtcagctccg aaaccttgaa aaagcggccg atatagagat
     7021 cgcgcagggc caggcggttg aggaccataa ccagggtaag ggcgatgaga tgccagaaag
     7081 tgggccaaaa ttttcccaga aaggccagca cgagggcgcc gatggcaagg atgatcccca
     7141 caatcaggat gagagtgtag aggagattcc cgcccatgaa agcgagcata aagtcgcgcg
     7201 gcagggcaat gagaaaccag atccccaccg ccacctgcac gctggtggca atggcgaaga
     7261 tgcgcagccc gttcctgacc tcctccccgc tgccgggcac ccccttgcgg gcgcggatct
     7321 gccagaccag gctgctgaag agcccggcga ccgccacgga ggcgatgacg aagtggaggt
     7381 agcgcggcca gagggccgga tccgagagat tgagaatggt gccgctgcgg ttggtgaaat
     7441 aggcggacca tttttccggc tgcagcatca gggtcatatt gtttgagtaa atgaaagtga
     7501 tatagaggaa aataagcgcg gccaggccaa tagccgtcct gccgagccag ttcacggcgc
     7561 tgcgggcgtg gatgtaggtg ccgtaatagg cgatgatcag caggggaatg accagcagcc
     7621 agtagagcgc catcagcacc gaactgctgt agatgaggtg gccgtagagg acctgcagaa
     7681 agagcagcgg ggcaacaccg agggtgaccc cgatcgccag ggtcgagggg atttttttat
     7741 agattgccga ggctgcaccg ttttcgcggg gagctttgct gaaaaaggca agaagcgaac
     7801 cgccaaggac cacattgatg aagaggatat ggagcatgaa aagcaggacc ccgaggactt
     7861 caaaaaccgg ccatggcgca ggaatggcct cgagtgtggg aatgagaggt atgccatcca
     7921 tgaaagaacc tcctctgccg cttggtgttg aaggtgtgga agagcatcga tgcaaaacag
     7981 cccggcgagc ggtggctgga ctgcacctat attaaggata gaatttgact ttatcaacaa
     8041 taaaatggag ggcggtttga cgggagtcaa cccgccaggg cgcgcagcaa ggccggggcg
     8101 acgtcggtga ggctgtgcag gcgggaggcg atctcctcgc gcccgcagcc aaagagaagg
     8161 gtcatgacgg gattgcgggt gtggcccttg acagcgagat cctcgatgtt gccatggtca
     8221 ctggtgacca gcaccagggt gtggtcgaga tcgacgacgg cgagaatacc cgccaggaaa
     8281 tcctcaaggc gggtgagcac acccagggcg cgtgcgaaat cgcggctgtg gccggcgaca
     8341 tcactctgga aaaactcgaa gaggctgaaa tcgtactccc ggctcgccgc cccgatgatc
     8401 tcccccgcct cctgcgcggt gtaaaggggc acatcgaaac cgtggtcgat cagtccggcg
     8461 ccggtgatgt cgtgatagac cgaacggcgg gcgcggaggt cgtcgagatc gaagaaacgc
     8521 agccccgcat aaagattact cactgtggtg accgagagac gattgatgat ctggaaggga
     8581 tcgagatcga aaaagggcgg ccggaaggtg ttgaggaaag cggcgcggaa gccgtgatcg
     8641 gtgaagcgtt tgaggatgtt gtgttcggcg atgacctgac gcagcgcctt gttggggaag
     8701 gcattgagat ggcggcccag cagccgggcc ccgttgactc cggtcagcag cgaggtctgg
     8761 ccggtggcgc tctgcggcag cccctccaca ccgagattgg cgtcgagccc catcgccagc
     8821 ccctccgagg cagtcgggcg cgggaaaatc ccggtgtggc actgccggaa caggttcagg
     8881 ctcgggtggc agcagggatt gatctccgga tcatccgccc cgatccccag cccgtcgatg
     8941 aaaaagagca ggagatggcg cagctcgggg atccggctca tttgtgcagt ttggcccagg
     9001 agttgaaaac catgaacaaa ccgagcagca ccatcactac tccgaaggcc aggcgcaggt
     9061 ggagctgatt gaggcggttg gcgatgtggg aagcggccca ggctccgatg aaaagacaga
     9121 tcgcgatgat gacagcggcc ttgagattga cattgttgtt ctgatagtaa accagaaccg
     9181 cagccagacc gaccggcggc aggaggacgg ccaggctggt gccggtggcc atctgctggc
     9241 tgaagccggc gaaatagacc agggccggca caatgatgat gccgccgccg atgccgaaaa
     9301 agccggagag ggttccggcg gccaggccga gaaggatata gagccaaagc gagggttcgg
     9361 aaccggtcag cataaattct ccaggatcgg ggtgaagccg gcacccgggt gcatcacatc
     9421 gggcgccggc atggaatcta gtagagctgc ttgatctggg tgccggggta gtaatgcttg
     9481 aggatcgcgc tcacactgtc gccccgtttg gcacggacgg cggcaccgag ctggcaaagt
     9541 cctacgccgt gcccccagcc ggcgccccgg aagataaagc tctcagccag gccatccttc
     9601 accccctcct tctcgatggt gaaacaggcg gaatagaggg tcgatttgga gagggcgcgg
     9661 cggatgttga gctccttgtg gatctttacg ctctcctcag tgccgatgca gttgatgctg
     9721 atcaggcggc ccgaggtgcc gcgctcgagg gggatgatgt cgaccagctg gccaatggag
     9781 gcgccggcat aatcggcgat cgatttttcc agatcggtgc gtgcgatgcg ctgctcccag
     9841 cggaaatatt tctccgcata gcggccgaaa tcgggattac cggcggcctc gaggttgcag
     9901 cagacctcag ggcgggagtt gacccagacc cgcacattct cctcgcgggt gaggtcgaac
     9961 cggccaatct cggtgggagt gtcgagccgt ccggtcagat agggctcgcc gtcgctgtcc
    10021 caaacctcgg cagcgctggc ggtatggccg ccgcacatcg ccgagtagct ggccgtacag
    10081 agggcgtcgt tgtaggtcag gaccagcccc ttggtgtcgt gcacggcctt ttcgacggga
    10141 tcgctgttct tgccgatgcc gatgtaggcc tggcagtgga catcatcgca gacatcgaat
    10201 tcggcaccgc gatggaaacg gccgaagctg ttgagaaaca tcgtcctcga gatgatcgcc
    10261 tgggccttga gggcctcctc gggaaaggag ttggccatct cgccggccac caccccgcgc
    10321 agatagagct cgagggggac gacgttgatg gcggtcagcc tgcctttggc attgatgcgc
    10381 agttccatct ccccgccgta ggtgcgggcc tcgttgcgcg cccagtggta accgctgccc
    10441 acctcgacat cgcgcagggt gatgttggcg ccggtcaggc gcagggcctg ggtaagaatg
    10501 atggtccgcc cggccggggt ctgcagggcg atcttgccca ttaccacatc atccatgctg
    10561 cggaccacct tgccctgcgc aagggcgggt gtagcggcga gatgacgctc ggcctccgct
    10621 gtggtggcga aagagcggtc gaggagaacc cggtgataga tgcggtcgat caggttcttt
    10681 gtgcccgcct tgagctgctc accctggctc ttcacggtca ccgcaagccc cttggcgcgc
    10741 agggccttgg cccgttccgc tgcgcggtcg ctgtccagcg actcgtagag ctgaatctga
    10801 taggtcagtg cgccgggcac gagggtgtgc acggtcgcct tccagagccc cttttccagg
    10861 gcctcgaaat tttccttctc gtcatagcag acgacggaaa attcgcccgc cggctcgaaa
    10921 tagaccgcct cctgatcctc gagaatgccg acgcggatga tcggatcgcc cgctaccggc
    10981 acatagcgca tctgggtaca tccggcggcc acaagcagca ccgccccggc cagaagcttg
    11041 cagagtgttt tcataaactt ccctctaccc ctgataacca aattcccgta aaaaagtatc
    11101 attcttgcgc catttatcgc gtaccgctac ccacagttcc agaaaaacag gccggtcgag
    11161 aaaggcttca atgtcagcgc gcgcctcctc ccccacccgg cgcagcatcg ccccgccccg
    11221 gccgatgatg attcccttct ggctctgccg ttcgacgacg atgcgagcct tgatgagatc
    11281 cttgcggtcg ggccggcctt ccctgaactc gtcgatgatc acagcggtcg aataggggat
    11341 ttcctcgcca aaatgggcga aaatcttttc gcgaataatc tcgctgacaa aaaatcgctc
    11401 gggatgctcg gtcagggcat cctgatcata aaaggggacg cccgccggca ggcgcgccgt
    11461 cagggtgtcg agcaggagat cgcagttttc gccggtgtgg gccgaaaaag ggacgatgcc
    11521 ggtgaaggga tgcagggcgc tccaggcctg ttccagagca ggcaacagcc ccctctcgac
    11581 cagatcgatc ttgttgatgc cgaggaggag gggtttgcgg gcggcggcaa gctcggccag
    11641 gatttcacga tcgcgctcct ctggcctggc cgcggcggca accatgaaga ggagcagatc
    11701 actgtccagg acggcacgtc gggccgtctc catcatcacc tcctgcagac gatagctggg
    11761 ggtgatcaga cccggggtgt cgagaaagat gatctgatag ctctccccgg tcagaatccc
    11821 catgatccgg tgacgggtcg tctgcggctt gggcgtggtg atcgagagct tgaactgcaa
    11881 cagccggttg acaatggtcg atttgccgac attgggctgg ccggcgaccg caacatagcc
    11941 ggaacggaag ggttcagccc ccttccggct ctcctgctcc ctggtcgtgc tcacagatag
    12001 gagtcgaggc tgtaggattc gtaaagcctc ttcaccagct catcgctgct gaccagcttg
    12061 aggctgtgga actggcagcc attgcgcagg cagccctcga cgattccccc cttgggcagc
    12121 tgcatggcga acatcggggc tgaacagacc gaacaacgct ggtcggcatc ctgaagggag
    12181 acgctgcagg ttgggcagag aaactcgacc agctcgccgt ccgggaccgt gatctgggag
    12241 atatttttaa aactgccata aatcggatcg agatggatga ggccggtttc gtcatgatag
    12301 cgggccagca ccgagaccga tgggtagccg ttgatgaggt ggttttcatc cataaggctg
    12361 tggccgttgg ggcagcttgc attctggatg cgcagcacct tgctgggcac ctccagttcg
    12421 acgacatctt tgtttttgtg cataacgcct ccgcacacaa gagagaatgg gggcggcgcg
    12481 cgggcaggcc gccgtcccgg ttggattagt aggatttggc cacgagcacg cgctggcctg
    12541 atcggccgcc gcagacgatg caggcgccct cttccttctc ttcatcgatg ggcaccaggc
    12601 ggatggtcgc cttgtgctcc tcctggaatc tggcttcgca gtccggggat ccgcaccagt
    12661 ggacccagaa aaagccgccc ggtttttcga tctgctccgc cagttctgca tagcgatcca
    12721 catggaaggt gttggcatcg cggaaggccc tggcgcgcac aaagaggttg tgctgcatct
    12781 cctcgagcag ggcttcgatg cgtgcgagca ggttctcctg cggcacggcg atcttttccc
    12841 cggtatcccg gcgcaccagg accacctgat tggcctccac atccttgggc cccatctcga
    12901 tccggagcgg cacccccttc ttttcccact cgttaaactt gtaaccgggc cggtactggt
    12961 cgcgatcgtc gatcttgaag gagatgctct cctcccagcc ggaggtcagt gcgcgcgctt
    13021 tggcgagcat catctcgcgc tcggaatcct ccttccagat cggcaccagc accgccttga
    13081 tcggcgcgag ccggggcggc agcaccagcc ccttgtcgtc gctgtgggtc atgatcaggg
    13141 cgccgatcag acgcgtggag actccccagc tcgtcgcata gaccagctcc agcttgccct
    13201 gctcattctg aaaggtgacg tcgaaggcgc gggcgaaatt ctggccgaga ttgtgcgagg
    13261 taccggcctg gagggccttg cggtcctgca tcatcgcctc gatgcagtag gtgcgcaggg
    13321 cgccggcgaa acgctgcgat tcggtcttga ccccgtaaaa gaccggcgcg gccatatact
    13381 cctcggcgaa acggcgatag acatcgagta tcttcagagt ctcctcctcc gcctcctcag
    13441 cagtggcatg agcagtgtgg ccctcctgcc agagaaactc ggtggtgcgc agaaaaagac
    13501 gggtgcgcat ctcccagcgc accacattag cccactgatt gatgagaata ggcagatcgc
    13561 ggtagctctg aatccagttc ttgtaggcgg accagatgat ggtctccgag gtggggcgca
    13621 ccaccagggg ctcgtccagc tttttaccgc cgccgtgggt gaccactgcg cattcgggcg
    13681 caaacccctc aacgtgctct gcttccttct tcataaaact ctcggggatg aaaagcggga
    13741 agtaggcgtt gacgtggccg gtggccttga acatcttgtc caggtgtccc tggatcatct
    13801 cccagatggc atatcccgtg ggcttgatga ccatgcatcc cttgacggcg gcgtgctctg
    13861 ccagatcggc ggcggccacg acatcgagat accactgtga ataatcggct gcgcgcgtgg
    13921 tgatttcttt agccatagta tcctgttctt ttttttccgt tagtggtttg agccgggccg
    13981 ggaggtgcat ccggccggct cggcagcgag ctgctgcctg acctgaaaat agcacgcctc
    14041 cgcactcgcc atcccctcca ggcagagcag ttcgcggcgg cggcgctcct ggccgttgct
    14101 gcggtcgatg acatcgacaa gggcgccctg cggacagtgc aggcacttgt ccggctgcct
    14161 gggcagggcg agggcactct gccgggccac cagggcgctg ctgttgtgcc aggtctccac
    14221 ccctgaacgc gacatcacct cgatggcgcg caggcgggga aagagcaggc ggccctgatc
    14281 gatggtaaag gcggcgcggg catccatctc gtccccgggc gcgagggcga ggcgctcctg
    14341 ctgctggggg gagatggcag cgtagaaata atcctcaaag gcatccagac cgataaagcc
    14401 ctcccgggca atgagaagat agccgtggag gtgcagccgg gcgccgtgga catcgatctg
    14461 cttgtgaaag cgcggcttga gcgagtcgat gcggaaggag atctcccgct cccgcccctg
    14521 gttctcctcc acccactcgt cgaaggcggc cagatcctgc tgaaagaggg cgtactcggc
    14581 gggtgagagg gtgatacgcg gctgataatg gatcttttcg tcgcgatagt gggcgcagcc
    14641 gggacagagt ctccccatgc ggttgtagcc gcggctgcag cgcttgccct tgttgagcag
    14701 ggcgcaggac cagcggaact caaggcagcc ctgggggtag cactttctgg cacccagcac
    14761 atgccaggcg gaaacccggt gggcaaaggc ggcatgacgg gcgtaatggc aggtgaaaac
    14821 gtcggtcctt ttgtagggat tcttcatgcc ctgccctcat aactccttta aataatttga
    14881 aatataatta ctctggccct gagaagcaag aaaattcttg ttcccgtgtc tcgggggcac
    14941 cctggccagt ttccttgcta cccccaaaat ctcggtccca cccagagtta attaattaaa
    15001 aaatagtttt atgcttgcca gaaacaccat gtaaataagg ctgcctcaat gcccagggcg
    15061 caagccgaga gtatgctgaa aatcgaacgg aggattgtgg tatgcggcgc caggaggcag
    15121 gtgggatcca gatttggggg gcaccctggc cagtttctcc ttgcaattga gttgaatttt
    15181 cataaattaa gaaaatttaa tcttcccgga aaaggaccgg atcatgaact tttcgcccct
    15241 cgatttcgcc atcatcttcg cctatctcgc cggggtcaca gccctggggg catggttcgg
    15301 caagaagcag agcagcacca ccgactattt tcttggcggc cgcgacctgc cctggctggc
    15361 catctgcatt tcagtggtcg ccacggagac cagtgtgctg accttcatca gcatccccgg
    15421 tctggcctat ctcaccaacc tcaactttct ccagatcgcg gccggctatc tgctcggccg
    15481 catcgtcatc tcttttgtgc ttctgccccg ctactatcgc ggagagatcc agaccgcata
    15541 ccacctgctc ggcaaccgct tcggctcgcg catgcgcagc ttctcctcga tcgtcttcca
    15601 gctgacgcgc ctgctcgctg acggggtccg cctcttcgcc gcctccatcc cgctgcacgt
    15661 gatcacaggc tgggattaca gtaccagcat catcgtcatc gccatggtca ccatcatcta
    15721 caccttcatc ggcggcatcc g
//
LOCUS       DAOPWU010000035        15647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3834_length_15647_cov_5.381285, whole genome shotgun sequence.
ACCESSION   DAOPWU010000035 DAOPWU010000000
VERSION     DAOPWU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15647
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3834_length_15647_cov_5.381285"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..862)
                     /locus_tag="PKM23_03500"
     CDS             complement(188..862)
                     /locus_tag="PKM23_03500"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNY90571.1"
                     /translation="MLQEFSTIFVTGITFEQVATNLLFALLCGLAISWIYRLTYRGPG
                     FAISFVHALIYLAMITAVVIMVIGNNLARAFGLVGAMSIIRFRTAVKDTADIVFIFFS
                     LAIGMAAGVGMHKLALGATMLIGLVMIMMAKSTAVLPMGRKFLLQFSLLGDKPDAVPY
                     EIILKEYCRTHKLINVKTEEEENLYEISFYVDLRDEQKHYELVKELRRLPGMLRVNLF
                     YDEEAI"
     gene            complement(928..1698)
                     /locus_tag="PKM23_03505"
     CDS             complement(928..1698)
                     /locus_tag="PKM23_03505"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNY90572.1"
                     /translation="MFRYEFKYLVPFNRIDALRRRIVPFVQEDKFAGRRADHQYVVRS
                     IYYETTGYRSYVEKLAGIERRVKVRIRGYNRPEPESSVFLELKRKHGPFIRKNRAPVL
                     WSELPDLLRDRDVERHVRVRRAFELGKEDARQFFYQVIGKGMSPAILIAYEREAYFAT
                     FDRRIRLTFDKHIRFRLTQDPGILFEERSLTPLFRSHFVFEVKFYDSLPEWLVDVISE
                     FSLQRQAVSKYTLSLERARALHPGRHGRDKSSLLLPAW"
     gene            complement(1704..2759)
                     /locus_tag="PKM23_03510"
     CDS             complement(1704..2759)
                     /locus_tag="PKM23_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90573.1"
                     /translation="MNKLTPTSMVRWLVIAAALLLVLLLPFRIPYTIKTIAKVQPRRE
                     WLVVRAEDGQISTHLWNHQSATLERVTVTQMLRGDATEFQILPGVLGSAEVAEGDTIA
                     ILHSADLVQAIAALQGDLTAARGSLLALSTGEKLPMIEEAQHQIEHAVVMAQEQKRVA
                     ERLAELHARQLVPFQEYELALATQQAFEANIAVARSRLASIQTGAKKEEIAAMEAQIA
                     ALEKELAALQQKAEGYLLRSPLSGTRIQHFASDTLLAVAETGSCILTMPVRARDRSLL
                     IGGEEVTIRASESRISRKGSVVFINPMLELLNGEQVFWVVARLEQPTGLISGLFVPCE
                     LKCKSLSKWQYLQRFME"
     gene            complement(3009..6587)
                     /locus_tag="PKM23_03515"
     CDS             complement(3009..6587)
                     /locus_tag="PKM23_03515"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNY90574.1"
                     /translation="MLHVRESYLVLLLATNFCFAAQPVRASAGGTGAAIQPLVLERAA
                     IRQLVTLNSGWEYLEEALPSVQALDDPALPWVKVDLPHTWNAFDAVDQEPGYRRDAGW
                     YRKEIVIPDNAGSRRFRLYFEGANLTTEVYVNGRKAGGYIGGYVGFTVDITSCLVKGR
                     NRIAVRVDNAINRDIIPSQKADFVIYGGICRDLWLQVLPADFIDRLLISTPKVSARSA
                     QVTARVRMINNSAEKQSASIAALIRDPQSKVVARSTAKVQLQPGENEFSLGFPALKNP
                     RLWSPDRPDLYCMEVELKTGASTDAVSERFGCRWFEFAEKGPFYLNGRRLLLRGTHRH
                     EEYAGYGSALPDSLHLRDMRMIKEMGANFVRLGHYPQDPAVYRACDELGLLVWDELPW
                     CRGGMGGPVWQNHTRRLFREQIEQNYNHPSIILWSIGNELYWLPDFPGGDHPDSLRAF
                     AGGLHALAHELDPGRLTAVRKFTGGEEITDLFSPSIWAGWYSGVYASYEKALTAARSR
                     YSRFIHAEYGGDSHVGRHTENPVDGRGIVKEDEWAEQPNMVNVARIAEEGDWSESYIV
                     DLLDWHLSVSERLDWLSGTAQWAFKDFATPLRPENPIPYVNQKGLVDRAGKPKDAWYL
                     FKSWWTENPQFCHIESPTWRERVGRPGESLEVCVFSNCAKVELRLNGASLGNKTRVAG
                     DFPANGLRWQVAFNEGKNHLLAIGFGGESETARDTLTAQYTVRKNGKPEEITLTAQKR
                     EDGSYLVIARVVDSQGQRCLDYNKRVYFSAAGAGRLLINYGTPTRSEVIEFANGQAAI
                     EFIPGLGRAVIEARNQDFKGAWIVLPPEPLPPAQLESERERVLRKADAALQKEPVTLT
                     SATCARSAGGRHDYYSEGDYWWPDPANPEGPYIRRDGLTNPDNFVAHRELLRHMSMEV
                     AALTTAWKISGEERYARAALRHLRAWFVDDSTCMHPHLTYAQAIKGITPGRGVGIIDT
                     IHLLEVARSASLLKNSPAMAPELDRRITAWFSDYLAWLTTSANGIDERERKNNHGSCW
                     VLQVAEFARLTGNDTLLAFCRDRFKTVLLPLQLAADGSFPLELERTKPYGYSLFNLDA
                     LAILCEILSTPEDNLWDYQLDDGRGMKKAMEYIYPYIADKNRWPHPPDVMYFEEWPLR
                     HPSLLFAGLAWREEGYLELWRRLPPEPATEEGSRNFPLRYPLLWLD"
     gene            complement(6609..8333)
                     /locus_tag="PKM23_03520"
     CDS             complement(6609..8333)
                     /locus_tag="PKM23_03520"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNY90575.1"
                     /translation="MVNYDTTTGQVISRETHQGYSDSSTWSRGQAWALYGCTMIYRES
                     RDERYLRQAEKIADFILNHPRLPRDGVPWWDYDAPGIPDEQRDASAGAILCSAFYELS
                     TLAKEGERYRQAADRILGSLSSPAYRADPGANGHFLLKHSVGSKPGKTEIDVPLIYAD
                     YYFLEANLRLLALMKAGGAGAGPGVSAEAGIGKADDPLLIDLAALKGVKSRLAAEEKS
                     LQPALKKLLREADEMMNARPLSVMDKAAVPPGGDKHDYMSLAPYWWPDPEKSDSLPWI
                     RRDGEVNPEVNRYDKLPLRTTDVQVTTLAIAWFLSGREAYARQAARLLRVWFLDAATK
                     MNPHLEYAQSIPGRNSGRGAGIIEGRSFFRMADAARLLAGSASWTTNEQLALEEWLGR
                     YYRWLQESKAGREEAAAKNNHGTWYDVQAAFLALYLGDGPAARRILEKFPSERIALQI
                     EPDGRQPRELARTRALSYSIMNLEGFFAAGILAERLGLDFWHYETPDGRSIRKALDFL
                     MPYLSGEAKWPWSQITGIEGGMEELIALLRLAAEKYGDPRYRQAAAGLPGGIKPASRV
                     NLLTPALP"
     gene            complement(8216..11164)
                     /locus_tag="PKM23_03525"
     CDS             complement(8216..11164)
                     /locus_tag="PKM23_03525"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNY90576.1"
                     /translation="MSSAQIRKGRLRCPGILASLGFLSPAAAAGRLFLLITLTLSVAR
                     AQTIPSDAELLAGLRPCLPALAEVYALRDQGQTEEALHRLAGYLREQSACRYYFDWQR
                     FGELFAAYCKRYPLKRSEHLRLAREQMTTYPADTEWMLPFRNLQGKEVTAYELRHLAR
                     QNKSRDIALMHHYERDTRYLHYWLAQVEDLNRAFKEGAYDQAGNAIYENLRGGMRVQN
                     WLFAHHVFLAALEYDEQAQLLLIRTLFHHGLMLEKGTQRYRSGNHHTKGLAALFELAV
                     LFGDFRAAEAWRAQAIAGLTRHMHEEINPDGFQFERSVHYHIGDIDNYFRVYQLAQIN
                     QIKLPEEFILRFRRMFTALAQLARPDRRLPVLQDDTDAAYSEYNEIYIPMSAGALLFG
                     DPLFRYFAGDAIPAELYWLTRPEQQGRLLQQSGVQPEFSSLALKESGYYCMRSGWEQN
                     DLHMVISAGLSRIKPDHQHGDMLGVVAWANGRDILPNYQVSYNTPEYPFWKNSWVKNV
                     ALADSIPLGRKWIPNEGGSGFGKWGVLPTPQVLEWRTAPEFDYFRGTHDSYDSLGIHY
                     FREVLFIKSGFWIIRDHFRSEGIHSWQQMWQGHYQRQNARCVVAQFADGSGLEIIQLD
                     APADTILFGVHRDKGSVLFQTRSTGNYTFTTLLHPFASGGRRIDSSAGLAVLQIGPWW
                     ITRNENGGLLLSRESPGASYLLPAEEFIILPASAPQSRASANPVSESRELKPARVADL
                     RMMVTVQENPLTATARANLDLALQQYSGMLTAIAGADRNPRTITPEGTAKLVPARDWT
                     SGFFPGALWLLYADSGDPHWRAAAEKQTALLEEEQYDRTTHDLGFKLYCSYGQGLRLT
                     GNEHYREVLLQAARTLAGRFNPTVGCIRSWDHNRSRWQYPVIIDNLMNLELLFWAACS
                     GGDSSLYHIAMTHANTTLKIISGRISAPGMWSTMTRLRGRSSAGRPIRATAIHRPGAG
                     ARPGPSTAAP"
     gene            complement(11161..11490)
                     /locus_tag="PKM23_03530"
     CDS             complement(11161..11490)
                     /locus_tag="PKM23_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNY90577.1"
                     /translation="MKNYIHSHEAVIEEVSPGIRRQILGYDTNLMLVRVQFAADAVSA
                     AHSHPHQQVTYVEKGVFEVTIGDEKQLLRRGDCFILPSGIQHGARCLEEGVLIDTFSP
                     AREDFLK"
     gene            complement(11549..12826)
                     /locus_tag="PKM23_03535"
     CDS             complement(11549..12826)
                     /locus_tag="PKM23_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY90578.1"
                     /translation="MKKINGLRWWIIALVALATVINYIDRSALAIMWPDINKDIGLGK
                     EQYAMIMTMFMIAYAISQTLSGKLYDWIGTRMGFVISIVVWSLSAALHAVARGAASFS
                     FFRFMLGLGEAGNWPGATKSNAEWFPIHERAFAQGIFNAGASIGSIISPPLIAVLYLL
                     IGWKLTFVVIGGLGLLWIIPWLFVNRALPGKHPWLTEEEKAYILEGQKTVESGKADHP
                     ERALSWGEMLKYRQSWSVIVSRFLLDPIWWLFVIWLPIYLNDRFQFDIKQIGLSAWVP
                     YVGAATGSLFGGWLSGFLIHKGWNIDRARKGTILLGGLFMLPCLVFAAYASTPVAAIL
                     LIALILFGFQISINNIQTLPSDFFSGKTVGSLAGLGGTSAALGTIIVTNLVPILSRAG
                     YTPVFLTGALLVPLSIIAVLLLGGRIRRVDLPS"
     gene            complement(12878..15061)
                     /locus_tag="PKM23_03540"
     CDS             complement(12878..15061)
                     /locus_tag="PKM23_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNY90579.1"
                     /translation="MRLTAHIIIAALLICSVVLTAGEHPSLVITVGEAAAIREDMGKY
                     PLFVRSFEAMKARVETALNRPIEVPPPGEGGGYEHERHKQNYREMQEAGIYFTITGDK
                     RAAGFIRDMLLKYADLYPNLGRHPQAKDQAPGKLFHQMLNETLWLTNAAMAYDCVHDQ
                     LTPAVRAGIEKNLFRPMIAWFTNENRKEFNRIHNHGTWTVCSIGLIGYVLGDRELVEM
                     ALYGTDKDGQGGFLKQLDLLFSPDGYYMEGPYYARYALQPFFIFAEAIERNQPELKIY
                     EHRGQILKKAWYSAVQTTFPNGIFPPINDASRTMDIRDSGMILTNSLAYARYGADPQL
                     LPVAQIQQEVPVSDAGLILARAFARAGEVPGMTWPSIEFTDGWDGRQGGLGILRSGEG
                     REQSMLLMKYGVHGEGHGHFDKLQFIFYDQGREVIPDYGFCRWINIEPKMGGRYLPEN
                     KSWAMQTIAHNTVTVDRKCQNDGSRKEADEMSGQRHFFETGDPRVQVMSARAEGYYPG
                     VSMQRTMLLVRDERLPYPVVVDLFRVVSDRVHCYDYPIHYRGQLIAADFNYQAFGSEQ
                     QPLGTEAGYQHIWREASAAVAGPLRLTWLDGNRYYTLLTAGAEAGEFFFGRSGASDPS
                     FNLISEPIFILRKEGQNYLFASVIEPHGWFSEAHEQSRDARGRLTTVKVIGHDETGSV
                     VEVEGANNLRWRIMVSNGPASSTAVHTMNLNGTSFSWTGNYRFEF"
     gene            complement(15108..>15647)
                     /locus_tag="PKM23_03545"
     CDS             complement(15108..>15647)
                     /locus_tag="PKM23_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNY90580.1"
                     /translation="LVDAALALGRIDILINNAGGLVKRVPVAEFDEAHYQTVFDVNVR
                     TAFLMSHLVLPQMKAQKSGKIIHFSSQAAHDGGGPGAAAYAASKGAIWTFTKGLAKEV
                     ASLGITVNAVSPGFIAQTAFHNTFTSKEAQERMPSLIPLGRVGTAEDVAKVVFFLASP
                     LSDYLTGQSIEVNGGLYMP"
BASE COUNT         3037 a         4384 c         5110 g         3116 t
ORIGIN      
        1 gaattttcaa agttaatatt tataatctgt taaaatattt ttcagcggcg actaaataaa
       61 atggaggggg aggcaggcga ccggggccgg tgaaatttgg catccgttgc agttatcgct
      121 tgtttttatc cgctgcggag cagactcgta tcgtctgatc tggatcagcg tatggcgaaa
      181 cggggtttca gatcgcttcc tcatcataaa agaggtttac ccgcagcatg ccgggcagcc
      241 tgcgcagctc tttgaccagc tcataatgct tctgctcatc gcgcaagtcg acgtagaagg
      301 aaatctcgta gagattctcc tcttcttccg tcttgacgtt gatcagcttg tgggtgcggc
      361 agtactcttt caggatgatt tcataaggca ccgcgtcggg cttgtcgccg agaagggaaa
      421 actggagtag aaacttccgc cccatcggca gaaccgcagt gcttttggcc atcatgatca
      481 tgaccagacc gatgagcatg gtggcgccga gggcaagctt gtgcatgccg acaccggcgg
      541 ccatgccgat ggccagactg aagaaaataa agacgatatc ggcggtatcc ttgacggcgg
      601 tgcggaaacg gatgatcgac atcgcgccga ccaggccgaa ggcccgggcg agattgttgc
      661 cgatgaccat aatgacgacc gcggtgatca tcgccaaata gatcagggcg tggacgaagc
      721 tgatcgcgaa tcccggtccg cggtaggtga gtcggtagat ccaggagatc gccagaccgc
      781 acaacagggc gaaaagcaga ttagtggcca cctgttcaaa ggtgatgccg gtgacaaaga
      841 tggtgctgaa ttcctgcaac atggttttgg attgatcctc ccgaaacgtt ccagccgagc
      901 ggttccaata cggccgtgat tctttcttta ccaggcgggc agcagcaggg atgatttgtc
      961 cctgccatga cgcccgggat gaagagcccg ggcgcgctcc agcgacaggg tgtattttga
     1021 caccgcctgc cgctgcaggc tgaattccga tatgacatcg accagccact cgggcaggct
     1081 gtcgtaaaat tttacttcga agacgaaatg gctgcggaag agcggtgtga gtgatctctc
     1141 ctcgaaaagg atgcccggat cctgcgtcag gcggaaacgg atatgcttgt cgaaggtcag
     1201 ccggatgcgg cggtcaaagg tcgcgaaata ggcctcgcgc tcataggcga tgagaatcgc
     1261 aggagacatg cctttgccga tgacttgata gaaaaattgc cgcgcatcct ccttgcccag
     1321 ctcgaaagcg cgccgcacgc gaacatggcg ctccacatcg cggtcgcgga gcaaatcggg
     1381 cagctccgac cacagcaccg gcgcccggtt cttgcggatg aaggggccgt gtttgcgttt
     1441 gagctcgaga aagacgctgc tctccggctc cggccgatta tagccacgga tgcgcacctt
     1501 gacccggcgc tcgattccgg ccagcttttc cacatagctg cgatagccgg tggtttcgta
     1561 atagatactg cggacaacat actgatggtc tgccctccgc ccggcgaatt tatcctcctg
     1621 gacgaagggg acgatccggc ggcgcagggc atcgatccgg ttgaaaggca ccaaatattt
     1681 gaactcgtaa cgaaacatgc agcctattcc ataaatctct gcaaatactg ccatttggag
     1741 agggatttgc attttagttc gcacggaacg aatagtccgg aaatcagacc ggtcggctgc
     1801 tcgagacgcg cgacgaccca gaagacctgc tctccgttga gcagttccag catgggattg
     1861 ataaagacga cagaaccctt gcggctgatg cggctctcac tggcgcgaat ggttacctcc
     1921 tcacccccga taaggaggga gcgatcccgg gcgcgcaccg gcatggtgag aatgcaggag
     1981 ccggtctctg cgactgccag cagagtatcg gaggcgaaat gttggatcct ggttccggag
     2041 agcggggagc gcaagagata gccctccgcc ttttgctgta aggcggcgag ttctttctcc
     2101 agtgcggcga tctgggcctc cattgctgca atctcttcct ttttggcccc ggtctggatg
     2161 ctggccagac gggatcgggc gacagcaata ttcgcttcaa aggcttgctg ggtggccaga
     2221 gctagttcgt actcctggaa ggggaccagc tgcctggcat ggagttccgc cagccgctca
     2281 gcaaccctct tttgctcctg cgccatcacc accgcatgtt cgatctgatg ctgagcctcc
     2341 tcgatcatgg ggagtttttc ccctgtggag agggccagca gggaacccct tgccgccgtc
     2401 aaatcgccct gcaatgcggc aatggcctgc accaggtccg ccgaatgcag aatggcgatg
     2461 gtatcccctt cggcgacctc cgccgaaccg agcacccccg gcaggatctg gaattccgtg
     2521 gcatcaccgc gcagcatctg ggtgaccgtc actctctcca gggtcgctga ttgatgattc
     2581 cagagatggg tggaaatctg gccgtcttcg gcgcgcacga ccagccattc acggcggggt
     2641 tggacttttg cgatagtttt gatggtgtag ggaatacgga aagggagcag caggacgagc
     2701 agcagggcgg cggcaatgac cagccagcgc accatcgatg taggggtcaa tttgttcatg
     2761 aaatctgtcc ggcagagtgt aatgcgaaac aatggcgtat cggcccggcg gaggcgtcac
     2821 cctcttcacg cccgcagcat ccggccctgt ccgcgtataa ctatttgtta atgaaacgaa
     2881 atatattaat tatgccggag gaatgcaagg caaaagccgc gaggcacgcc ctccgcaggc
     2941 agccgtgata gggggcgctg gaggagtatc catcaaaagc gcagaccgaa atcctgggcg
     3001 gtgtctgttc aatcgagcca gagcagggga taacggaggg gaaaattcct cgatccttct
     3061 tccgtggcgg gttcgggcgg cagcctgcgc cagagctcga ggtatccttc ttcacgccag
     3121 gccaggccgg cgaagagcag gctgggatga cggagcggcc actcctcgaa atacatgacg
     3181 tcgggcggat gaggccagcg gttcttgtcc gcgatatagg ggtagatata ctccatcgcc
     3241 tttttcatcc ccctgccatc gtccagttga taatcccaca ggttgtcttc cggcgtcgag
     3301 aggatctcgc agagtatggc cagtgcgtcg aggttgaaca gggaatagcc gtagggtttg
     3361 gtgcgctcga gctcgagggg gaagctgccg tcggcggcaa gctgcagcgg cagcagcacg
     3421 gttttgaagc ggtcacggca aaaggccagc agggtgtcgt tgccggttag gcgtgcgaac
     3481 tccgccacct gcagcaccca gcagctgccg tggttgtttt tgcgttcgcg ctcgtcgatg
     3541 ccgttggcgg aggtggtcag ccaggccaga tagtcgctga accatgcggt gatccgccgg
     3601 tcgagctccg gggccatcgc cggtgaattt ttcagcagcg aggccgacct ggctacctca
     3661 agcagatgga tggtgtcgat gatgccgacg ccgcggcccg gcgtgatccc cttgatcgcc
     3721 tgggcgtagg tgagatgggg atgcatgcag gtgctgtcgt cgacgaacca ggcgcgcaga
     3781 tggcgcaggg cggcgcgggc gtagcgctct tctccgctga tcttccaggc cgtggtgagg
     3841 gcggccacct ccatgctcat gtgccgcagc agctcgcggt gggcgacaaa attgtccgga
     3901 ttggtcaggc cgtcgcggcg gatgtagggt ccctccgggt tggccggatc cggccaccag
     3961 taatcgccct cggaatagta atcgtgcctg ccgccggcgc tgcgtgcgca ggtggccgag
     4021 gtgagggtga ccggctcctt ttgcagggcg gcgtccgcct tgcgcagcac ccgctcgcgt
     4081 tcgctctcaa gctgtgcggg agggagaggt tccgggggga ggacgatcca ggctcctttg
     4141 aaatcctgat tgcgggcctc gatcaccgcc cggccgaggc cggggatgaa ctcgatggcc
     4201 gcctggccgt tggcgaattc gatcacctcg ctgcgtgtag gggtgccgta gttgatcagc
     4261 aggcggccgg cgcctgcggc gctgaaatag acccgcttgt tatagtccag gcagcgctgc
     4321 ccctggctgt cgacaacgcg ggcgatgacc agatagctgc catcctcccg tttttgcgct
     4381 gtaagggtga tctcttcggg tttgccgttt tttcgaacgg tatattgcgc cgtcagggta
     4441 tcccgtgctg tttcgctctc cccgccgaaa ccgatggcga ggaggtggtt tttaccttca
     4501 ttaaaggcga cctgccagcg cagtccgttg gcggggaaat ctccggccac ccgcgttttg
     4561 tttcccagag aggcgccgtt caggcggagc tcgactttgg cgcagttgct gaagacacag
     4621 acttcgaggg actcaccggg ccggccgacc cgttccctcc aggtcggcga ttcgatatgg
     4681 cagaattggg gattctccgt ccaccagctt ttgaacaggt accatgcatc cttgggtttt
     4741 ccggcccggt ccaccagccc cttctggttg acgtagggaa taggattttc cggccgcaga
     4801 ggtgtggcga aatccttgaa ggcccattgc gccgtcccgc tcagccagtc gaggcgctct
     4861 gatacggaga gatgccagtc caggagatcg acgatgtagc tctcgctcca gtccccctcc
     4921 tccgcgattc ttgccacgtt gaccatgttg ggttgttccg cccattcatc ctccttgaca
     4981 ataccccggc catcgaccgg attttcggtg tggcggccga catggctgtc gccgccgtat
     5041 tccgcgtgga tgaagcggct gtagcggctg cgtgcggcgg tcagggcttt ttcatagctg
     5101 gcatagacgc ccgaatacca tcccgcccag atcgagggcg agaaaagatc ggtgatctcc
     5161 tcgccgccgg tgaatttgcg cacggcggtc agccgtccgg gatcgagttc gtgcgcaagg
     5221 gcatggagac caccagcgaa ggcgcgcagg gagtcgggat ggtcaccgcc gggaaaatcg
     5281 ggcagccagt agagttcgtt gccgatcgac cagaggatga tcgaggggtg gttgtagttc
     5341 tgttcgatct gctctctaaa aagccggcgg gtgtggtttt gccagaccgg gccgcccatg
     5401 ccgccgcggc accagggcaa ctcgtcccag accagcaggc caagctcatc gcaggcgcgg
     5461 tagaccgcgg ggtcctgggg ataatggccg agtcggacga agttggctcc catttccttg
     5521 atcatgcgca tgtcgcggag gtggagggaa tcggggaggg cgctgccata cccggcgtac
     5581 tcttcgtggc gatgggtgcc gcgcaggagc agccgccggc cgttgagata aaaaggccct
     5641 ttttccgcga attcgaacca gcggcagccg aagcgctctg acacagcatc cgtggaggcg
     5701 ccagtcttga gctccacctc catgcagtag aggtctggcc ggtcgggaga ccagagccgt
     5761 ggattcttca gagccggaaa accgagggaa aactcatttt cacccggctg cagctggact
     5821 ttagccgtgg atctggccac caccttgctc tgcgggtccc tgatcagggc ggcgattgag
     5881 gcggattgtt tttctgcaga gttatttatc atccgcaccc gggcggtcac ctgcgctgaa
     5941 cgtgcgctca ccttaggggt actgatgagc agccggtcga tgaaatcggc gggcagcacc
     6001 tgcagccaga ggtcgcggca gataccacca tagataacga aatcggcttt ctgcgagggt
     6061 atgatgtcgc ggttgatcgc attgtccacg cgcacggcga tccggttccg gcccttgacc
     6121 agacaggagg tgatatccac tgtaaagccg acatagccgc cgatgtagcc tccagcctta
     6181 cggccgttga catagacctc ggtggtcagg ttggcgcctt cgaaatagag ccggaagcga
     6241 cgcgatccgg cattatcggg gattacgatc tccttgcgat accaaccagc atcgcggcgg
     6301 tagccgggct cctggtcgac agcgtcgaag gcgttccagg tgtggggaag atcaactttt
     6361 acccagggca gagcggggtc gtcgagagct tggaccgacg ggagggcttc ttcgagatac
     6421 tcccagccgg agttgagcgt gacgagctgt cgaatggctg cgcgttcaag taccaggggc
     6481 tgaatggcgg caccggtccc gccggccgag gcgcggacag gttgggcggc gaagcaaaaa
     6541 ttggtggcca gtaataacac cagatacgac tctcgaacgt gcagcatatc ggatttcctt
     6601 tgctggggtt aagggagggc gggggtgagc aggttgaccc ggctggcggg ttttattcct
     6661 cccggcaggc cggcggcagc ctggcgataa cgcggatcgc catatttttc ggccgccagg
     6721 cgtagaaggg cgatcagctc ttccatcccg ccctcgatgc cggtgatctg ggaccagggc
     6781 catttggcct ctccgctgag gtagggcatg agaaaatcga gggccttgcg gatgctgcgg
     6841 ccgtccggcg tctcgtaatg ccagaagtcg aggccgaggc gctccgccaa tattcccgca
     6901 gcgaaaaagc cctccaggtt catgatgctg tagctgaggg cgcgggtgcg tgcgagctcg
     6961 cggggctggc ggccgtcggg ttcgatctgg agggcgatgc gctcggacgg gaatttttcc
     7021 aggatgcgac gcgccgccgg cccatcgccg aggtagaggg cgagaaaggc ggcctggacg
     7081 tcgtaccagg tgccgtggtt gtttttggca gccgcctcct cgcggccggc cttgctctcc
     7141 tgcaaccagc ggtaatagcg tccgagccac tcctccaggg ccagctgctc atttgtcgtc
     7201 catgaggccg aaccggcgag caggcgggcg gcgtcggcca tgcggaagaa ggagcggcct
     7261 tcaatgatgc ccgcgccccg gcccgagtta cggccgggga tggactgggc atactcaaga
     7321 tgtgggttca tcttggtcgc ggcgtcgagg aaccagacgc ggagcaggcg cgcggcctgg
     7381 cgggcatacg cctcgcggcc gctgaggaac caggcgatgg cgagggtggt gacctgcaca
     7441 tcggtggtgc gcagggggag cttgtcgtag cggttcacct cgggattgac ctcgccgtcg
     7501 cggcgaatcc agggcaggct gtcgcttttt tcggggtcag gccaccagta gggtgccaga
     7561 ctcatataat cgtgtttgtc accgcctgga ggcactgcgg ccttgtccat caccgagagg
     7621 ggccgggcat tcatcatctc atcggcctcc cggagcagct ttttcagggc tggctgcagg
     7681 cttttctctt cggctgcgag tcgcgacttg acgcctttca gggcggccag atcgatgagc
     7741 aggggatcgt ccgcctttcc aatgccggcc tcggcgctga cgccgggtcc cgcgcccgct
     7801 ccgccggctt tcatgagcgc cagcaggcgc agattggcct caagaaaata gtaatcggca
     7861 tagatgagcg ggacatcgat ctccgttttg cccggcttgc tgccgacgct gtgcttgagc
     7921 aaaaaatggc cgtttgcgcc cggatcggcc cggtaggccg gtgaggagag gctcccgaga
     7981 atacgatccg cggcctggcg atagcgctcc ccctcctttg ccagggtgct caattcgtaa
     8041 aaagccgagc agaggatggc gccggccgag gcatcgcgct gttcatcagg aatgccgggc
     8101 gcatcgtaat cccaccaggg cacgccgtcc cggggtagac ggggatggtt caggataaaa
     8161 tccgcgattt tttcggcttg gcgcagatag cgctcatccc tgctctcgcg atagatcatg
     8221 gtgcagccgt agagggccca ggcctggccc cggctccagg tcgatgaatc gctgtagccc
     8281 tgatgggtct ccctgctgat gacctgcccc gtagtcgtgt catagttgac cacatgccag
     8341 gtgctgaaat cctcccggaa atgattttta gggtggtatt ggcgtgggtc atggcgatgt
     8401 ggtagaggga ggagtcgccg ccggagcagg cagcccagaa gaggagttcg aggttcatca
     8461 ggttatcgat gatgaccggg tactgccagc ggctgcggtt gtgatcccag gaacgaatgc
     8521 agcccacggt ggggttgaaa cgtcctgcca gggttcgcgc ggcctgaagg agcacctccc
     8581 ggtagtgttc gttcccggtc aggcggaggc cctggccata gctgcaatag agtttgaacc
     8641 ccagatcgtg ggtggtgcgg tcgtactgtt cctcttcgag cagggcggtc tgcttctccg
     8701 ccgccgcccg ccagtgcgga tcaccggaat cggcgtagag aagccagagg gcgcccggga
     8761 aaaaaccact cgtccagtcc ctggccggaa ccagtttggc agtgccctcc ggcgtgatgg
     8821 tgcgcggatt gcggtcggcg ccggcgatcg ccgtcagcat gccgctgtac tgctgtaaag
     8881 cgaggtcaag gttcgcccgt gcagtcgccg tcaaaggatt ctcctgcact gtgaccatca
     8941 tcctcagatc tgccactctg gcaggtttca attctctgct ctccgataca ggatttgcgc
     9001 tcgccctgct ctgcggggca cttgccggca gaatgatgaa ctcttcagcg gggaggagat
     9061 aggaggcgcc cgggctctcc ctggaaagga ggaggccgcc gttttcattc cgggtgatcc
     9121 accatggtcc gatctgcagg acggccaaac cggcggatga gtcgatccgg cggccacccg
     9181 aggcgaaggg atgcagcagg gtggtgaatg tgtaattacc ggtcgagcgt gtttgaaaga
     9241 gaacgctgcc cttgtcgcgg tgtacaccga agaggatcgt atcggcagga gcgtcgagct
     9301 ggatgatctc caggccgctg ccatcggcaa attgagcaac cacacagcgc gcgttttgcc
     9361 gctgatagtg cccctgccac atctgctgcc aggaatgaat cccttcgctg cggaaatgat
     9421 cgcgaatgat ccagaaaccg ctcttgataa agagcacctc gcggaaatag tggatgccga
     9481 gagagtcgta gctgtcgtgg gtgccgcgaa aatagtcgaa ttccggcgca gtgcgccatt
     9541 ccaggacttg cggagttggc aggacgcccc atttaccaaa gccggagcca ccctcattgg
     9601 ggatccactt gcggccgagg gggatgctgt cggccagggc gacattcttt acccaggagt
     9661 tcttccagaa gggatactcg ggggtgttgt agctgacctg atagttgggc agaatgtcgc
     9721 ggccgttggc ccaggcgacc acgccgagca tgtcgccatg ctggtgatcg ggcttgatgc
     9781 gggagaggcc ggccgaaatg accatgtgga ggtcgttctg ctcccatccc gagcgcatgc
     9841 agtagtagcc gctctccttc agggcgagcg aggagaattc aggctggacg ccggactgtt
     9901 gaagcagtcg accctgctgt tccggacggg tcagccagta gagctcggcc gggattgcat
     9961 cgccggcaaa gtagcggaag aggggatccc cgaagagcag ggcgccggcg ctcattggga
    10021 tataaatctc gttgtattcc gaataggctg catcggtatc gtcctgcaga acgggcaggc
    10081 gccgatcagg ccgggccagc tgtgccaggg cggtgaacat gcggcggaag cgtagaatga
    10141 actcttcggg tagcttgatc tggttgatct gcgccagctg atagacgcgg aagtagttgt
    10201 cgatgtcgcc gatatgataa tggaccgagc gttcgaattg aaaaccgtcc ggattgatct
    10261 cttcgtgcat atgccgggtc agcccggcga tggcctgggc ccgccatgct tccgccgcgc
    10321 ggaaatcgcc gaaaagcaca gcgagttcaa agagggcggc caagcccttg gtatgatggt
    10381 tgccggagcg atacctttgg gtgcctttct caagcatcaa gccgtgatga aacagggtgc
    10441 ggatgagcag gagttgggcc tgctcgtcat actccagggc ggcgagaaaa acatggtgcg
    10501 cgaagagcca gttttgcacc cgcatgcctc cgcgcaaatt ttcataaatg gcgttgcccg
    10561 cctgatcata ggccccctct ttgaaagcgc ggttgagatc ctccacctgg gccagccaat
    10621 agtggagata gcgcgtatcc cgctcatagt gatgcatcag ggcgatgtcg cggctcttgt
    10681 tctggcgggc gaggtggcgg agttcatagg cggtgacctc tttcccctgc agattcctga
    10741 aaggtagcat ccactcggtg tcggccggat aggtggtcat ctgttcgcgg gcgagccgga
    10801 ggtgttcgct gcgcttgagg ggatagcgct tgcagtaagc ggcgaagagt tctccaaaac
    10861 gctgccagtc gaaataatag cggcaggcgg actgctcgcg tagatagccg gctaatctgt
    10921 gcagtgcctc ttcagtctgc ccctggtcac gcagggcgta gacctccgcc agggcgggca
    10981 ggcagggcct gagccccgcc agcagctcgg catcggaggg aatggtctgc gcccgggcga
    11041 cagagagggt cagggtgatg agaagaaaga gcctgccagc cgccgcggcc ggggagagga
    11101 agcccaggct ggcgagaatt cctggacacc gcaggcgccc tttgcggatt tgtgctgaac
    11161 tcatttgaga aaatcctcac gtgccggtga aaatgtatcg atgagcacac cctcctccag
    11221 gcacctcgcc ccgtgctgaa tgcccgaggg caatatgaaa cagtcccccc ggcgcagcag
    11281 ctgtttttca tcaccgatgg tcacttcgaa gacccccttt tcgacatagg tgacctgctg
    11341 atgcggatgg ctgtgggctg ctgagacagc gtcggcggcg aactgcaccc gcaccagcat
    11401 caggttggtg tcataaccga gaatctggcg gcggatgccg ggagagacct cctcgatcac
    11461 cgcttcatgg ctgtggatat agtttttcat ggcatctctt tcgtgagttc gaattcgcga
    11521 tcaaaggccg gcatcccggc cgtcatcttc atgaaggcag atcaaccctg cggatgcggc
    11581 cgccgagcag cagtaccgcg atgatggaga gggggacgag cagggcgccg gtgaggaaaa
    11641 ccggggtata gccggccctg gagaggatcg gcaccagatt ggtgacgatg atggtcccca
    11701 gggcggcgct ggtgccgccg agtccggcca gggagccgac ggtcttaccg gagaaaaagt
    11761 cgcttggcag ggtctggatg ttattgatcg agatctgaaa gccgaagagg atgagcgcga
    11821 tgagcaggat ggcggccacg ggagtgctcg cataggcggc gaagaccagg caggggagca
    11881 tgaagagacc gccaaggagg atggtcccct tgcgggcgcg atcgatattc catcccttgt
    11941 ggatgagaaa gccggagagc cagcccccga agaggctgcc ggttgcggcg ccaacatagg
    12001 gcacccaggc ggagaggccg atctgcttga tgtcgaactg gaagcggtca ttgaggtaga
    12061 tcggcagcca gatgacaaag agccaccaga tgggatcgag gagaaaacgg gagacgatga
    12121 ccgaccagga ttgccggtat ttgagcatct ccccccagct gagggcgcgc tccgggtggt
    12181 ccgcctttcc gctctccaca gttttttgcc cctcgaggat gtaggccttc tcctcttcgg
    12241 tcagccaggg atgtttcccg ggcagggcgc ggttgacgaa gagccagggg atgatccaga
    12301 gcagccccag tccgccgatg accacaaagg tcaacttcca gccgatgagc agatagagca
    12361 ccgcgatcag tggcggtgag atgatcgaac cgatggaggc cccggcgttg aagatgccct
    12421 gggcgaaggc gcgctcgtga atgggaaacc attcggcatt ggatttggtg gccccaggcc
    12481 agttgcccgc ctcccccagc ccgagcatga agcggaaaaa gctgaagctg gccgctccgc
    12541 gcgccacggc gtgcagggcc gccgaaaggg accatacgac gatggagatg acgaatccca
    12601 tgcgggtgcc gatccagtca tagagcttgc ccgagagggt ctggctgatg gcataggcga
    12661 tcatgaacat ggtcatgatc atggcgtact gctccttgcc caggccgata tccttgttga
    12721 tgtccggcca catgatggcc agggcggagc gatcgatgta gttgatcacc gtcgccaggg
    12781 ccaccagggc gatgatccac cagcgcagtc cattgatctt tttcatacta tgcggttctc
    12841 cattttgcca ttcctggccg tttcggatgc ggggtaatta aaactcgaac cggtagttgc
    12901 cggtccagga gaagctggtc ccgttcaggt tcatagtgtg gacggccgtg gaagaggcgg
    12961 ggccgttgct gaccatgatc cgccagcgca gattgttggc gccttcgacc tcgaccaccg
    13021 agccggtttc atcatggccg atcactttca cagtggtgag gcggccgcgg gcgtcgcgcg
    13081 actgttcgtg cgcctcgctg aaccagccgt gaggctcgat cactgaggca aagagatagt
    13141 tctgcccctc tttgcgcagg atgaagatcg gttcagagat cagattgaag gagggatcac
    13201 tggcgccgct gcggccgaag aaaaactcgc ccgcctcggc cccggctgtg agcagggtgt
    13261 agtagcggtt gccgtccagc caggtgagcc gcaacgggcc ggcgacggcg gcggaggcct
    13321 cccgccagat gtgctggtag ccggcttcgg tgccgagtgg ctgctgttcg ctgccaaagg
    13381 cctgatagtt gaaatcagcg gcgatgagct ggccgcggta atgaatcgga tagtcatagc
    13441 agtgcacccg gtcgctgacc acccggaaga gatcgacgac aaccggatag gggagccgtt
    13501 cgtcccgcac cagcagcatc gtgcgctgca tgctcacccc gggatagtag ccttccgccc
    13561 gcgcgctcat cacctgcacg cgcggatcgc cggtttcaaa gaaatggcgc tgccccgaca
    13621 tctcatccgc ctctttgcgg ctgccgtcat tctggcactt tcggtcgacg gtcacggtat
    13681 tatgggcgat ggtctgcatg gcccaggatt tgttctcggg tagatagcgg ccgcccatct
    13741 tgggttcgat gttaatccag cggcaaaaac cgtaatcggg gatgacctcg cggccctgat
    13801 cgtaaaaaat aaactgcagc ttgtcaaagt ggccgtggcc ttcgccgtgg acaccgtact
    13861 tcatcaacag catggattgc tcccggccct caccggagcg caggatgccg aggccgccct
    13921 gcctgccgtc ccagccatcg gtgaattcga tgctcggcca ggtcatgccg ggcacttcgc
    13981 cggccctggc gaaggcgcgg gccaggatga gtccagcgtc gctcaccggc acctcctgct
    14041 gaatttgggc gaccgggagc agttgcggat cggcgccgta acgggcataa gcgaggctgt
    14101 tggtgagaat catgccgctg tcgcggatat ccatggtgcg cgaggcgtcg ttgatgggtg
    14161 gaaagatccc gttcgggaag gtggtctgca cagccgagta ccaggctttt ttaaggattt
    14221 ggccgcgatg ctcgtagatc tttaattcgg gctggttgcg ttcgatcgcc tcggcgaaga
    14281 tgaaaaaggg ctgcagggcg tagcgggcgt aatagggccc ctccatgtaa tagccgtcgg
    14341 gcgagaagag tagatcgagc tgcttgagaa agccgccctg accgtccttg tccgtgccgt
    14401 aaagggccat ctcaaccagt tcccggtcgc caagtacata gccgatgagg ccgattgaac
    14461 agactgtcca ggtgccatga ttatggatgc gattgaattc cttgcggttt tcgttggtga
    14521 accaggcgat cattgggcgg aagagatttt tttcgatccc ggcccggacg gccggggtca
    14581 gctggtcatg gacgcaatca taggccatgg cggcattggt gagccagagg gtttcgttga
    14641 gcatctggtg gaagagcttg cccggggcct ggtctttagc ctgggggtga cgtcccaggt
    14701 tgggatagag gtcggcatat ttgagcagca tgtcccggat gaagccggcc gcccgtttgt
    14761 cgccggtgat ggtgaaataa atccccgcct cctgcatctc gcgataattc tgcttgtggc
    14821 gttcgtgttc atagccgccg ccctcgccgg ggggcggaac ctcgatgggc cggttcaggg
    14881 cggtctccac ccgcgctttc atggcttcaa aagagcggac gaagagggga tatttgccca
    14941 tatcttccct gatcgccgcc gcctccccga cggtgatgac gaggctggga tgctctcctg
    15001 cggtgagaac gacactgcaa atgagcaggg ctgcgatgat gatgtgagcg gtaaggcgca
    15061 tgtatttcct ctcaggacag ttagcagttc agagttagac ggttgtttca gggcatatag
    15121 agcccgccgt tgacttcgat ggactggccg gtcagatagt cggagagggg ggaggcgaga
    15181 aagaagacca ctttggcgac atcctccgcc gtgccgacgc gcccgagcgg gatgagcgag
    15241 ggcatgcgtt cctgggcctc cttcgaagtg aaggtgttat ggaaggcggt ctgggcgata
    15301 aaaccgggcg aaaccgcatt gacggtgatg ccgagggagg ccacctcctt ggccagcccc
    15361 ttggtgaagg tccagatcgc ccccttggag gcggcgtagg ccgctgcgcc agggccgccg
    15421 ccgtcatggg ccgcctgaga ggagaagtgg atgattttgc cgctcttttg tgccttcatt
    15481 tgcggcagga caagatggga catcagaaag gcggtgcgga cgttgacatc gaagacggtc
    15541 tggtaatggg cctcatcgaa ctcggccacc ggcacccgtt tgaccagccc gccggcgtta
    15601 ttgatgagaa tatcgattct tcccagggcg agggcggcat cgaccag
//
LOCUS       DAOPWU010000036        15414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3901_length_15414_cov_6.108861, whole genome shotgun sequence.
ACCESSION   DAOPWU010000036 DAOPWU010000000
VERSION     DAOPWU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15414
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3901_length_15414_cov_6.108861"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PKM23_03550"
     CDS             <1..843
                     /locus_tag="PKM23_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY90581.1"
                     /translation="LAAYGEFIRSYPDWPETPDLYLRAGDLYLDRFFNLAAADSVYQR
                     LLQRPNLPVSYHLLALQKRAQCAIAGGDLRSAEKFISTLHRETPTGYGRQRQADLMLV
                     QIDLFEGRPGRALNKLEKLIGGAGGAGEAKADTVQNDLLELYLLLRSSRQDSLELVRY
                     GKALWLQRQRSHAAAFDTLAALLAGYRPGALAERARFLQVDLLRTMGRFPEALVISAA
                     LAADSSGLAPDFALMTMAGLHEELKEPEKARQLYESFLEKYPESIYIEQVRGHIRRLE
                     TRSR"
     gene            867..2123
                     /locus_tag="PKM23_03555"
     CDS             867..2123
                     /locus_tag="PKM23_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNY90582.1"
                     /translation="MRRLVFFIVWLFSATAFGQQLLVYMDHRQENHLKAYGLAYWVLD
                     HGVEVEWLLNYRGGSFLTAYSTATERELRVRGVSFSTLSGAEVSSIYAEIERENMEVV
                     KLEKAPAVAIYAPPNKQAWDDAVLMALDYAEIPYTVLWDEEVVRGELQKYDWLHLHHE
                     DFTGQYGKFYASYRNADWYKDEVRKNEAVARKLGFAKVSELKRAVAHGIRDFVAAGGF
                     LFAMCSATDSFEIALAASRVDIVDALFDGDPIEPGAQGKLDYTQTFAFENFKIETNPM
                     VYEYSDIDVPPSYAPVSRGAEGDYFTLFEFSAKYDPVPTMLSQNHVAAIKGFMGQTTG
                     FQKKLIKKSVTIMGEAEGTDQVKYLRGNFGRGTFCYLAGHDPEDFQHFVNDPPTQLAL
                     HKNSPGYRLILNNILFPAARKKELKT"
     gene            2188..2982
                     /locus_tag="PKM23_03560"
     CDS             2188..2982
                     /locus_tag="PKM23_03560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY90583.1"
                     /translation="MFKHRHIPPEQVIAAEPYTALAQIYDQVMNHVDYDHWARHLIRL
                     ARLHGVEPRTVLDVSCGTGTLCREFQSRGFRVLGCDASLPMVKIAAAFRSSRAAIPYW
                     CTAMDRVAIRTPVELVLSSYDSMNYLLEPQQWQSAFQRLHELLVPGGLFIFDISTLRN
                     STETFADYVHQEEFAEGSYRRTSLFDAERNIQYNYFEIELSSAPGCLYKEVHSQSIRA
                     LAQVEDFIALSPFTLLGQYANFTLRPGSERAERVHFVLKKGRETCM"
     gene            2997..3647
                     /gene="ftsE"
                     /locus_tag="PKM23_03565"
     CDS             2997..3647
                     /gene="ftsE"
                     /locus_tag="PKM23_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014073147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNY90584.1"
                     /translation="MVELQSVRVTYANGEGIDQITFSVAPGEMVFLVGPSGAGKSTVL
                     KTIYMDKQPAEGHVVVGDYNSLFIRKSEVPFLRRKLGIVFQDFRLFADRDVFDNVAFT
                     LEVIGARQKEIKRRVLRALADVGLSHKARKMPDELSGGEQQRVAIARAIVNEPLLLLA
                     DEPTGNLDPETAAGIMDVLLRINTRGTSILMATHNYDMVQRYRGRIIKINNGRLVA"
     gene            3669..4517
                     /locus_tag="PKM23_03570"
     CDS             3669..4517
                     /locus_tag="PKM23_03570"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNY90585.1"
                     /translation="MNLRHVLKETADGFRRAWGSCLLSILTIAFFLSLLGFIALVSAN
                     IDHFKQTLNDRLQLQVFISGTLDDQQIAALSQRISRISGVAKVSFVSREMAAEEFQKE
                     FGRELFAALEENPLPSSFVVRLGITGEDEGRIKQIAARIEEEAGVDEVVYHFQTLFTL
                     QRYARLAATTSWILLLFVTLGSLFVVSNNIRLVISARKQIISTMRLVGATPGFIRSPL
                     ILEGALQGAAGALLAGLALYFVDRLLAQLLPGLLHLPPESLIVLLLLGCFLGFLGSLL
                     AIKRYL"
     gene            4631..5665
                     /gene="hrcA"
                     /locus_tag="PKM23_03575"
     CDS             4631..5665
                     /gene="hrcA"
                     /locus_tag="PKM23_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNY90586.1"
                     /translation="MTLDDLTERERQVFLSIVQSFIDTAEPVGSRYLSKFAELNLSPA
                     TIRNVMADLEEKGLIRQPHASAGRVPTDGGYRAYVDGMIGGVKLSPSERRSIVEQLNR
                     FAEDVDLIIDQASHVLSNISSQLGVVLAPRFNQGRLEKIELVSVSEEKILVILTIASG
                     LVKTIIVEMDEKVPGHLLEAARQILNERLHGLTIQEFQSTFEERFSDLDENSKRVLGK
                     IRSRAEKLVGYDPVASFHVAGAGKIILQPEFASQEKVGVILNLIDRRDLLLRVLEESD
                     CSGVSIVIGDENKQEVMKHCSIITTTYSIGGATGTIGVIGPTRMQYAKIIGLVQFMAE
                     TLGYVLNKQN"
     gene            5688..6287
                     /locus_tag="PKM23_03580"
     CDS             5688..6287
                     /locus_tag="PKM23_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNY90587.1"
                     /translation="MKEEQKNSDREQTVADLEESLQEEPREQSGPEEPSADGAGAETE
                     KAVDADAAAIAALTAENLKLKDQLLRLAAEFDNYRKRSDRDMQSYISNANYDLMARLL
                     PALDDLDRIVAAAGGGAELAALVDGINLLHKNILKVFQDGGLEPMQTIGEEFDPLLHD
                     ALLHIEVSGSEPNRVVEEHKKGYFFKGRVLRHAQVVVSK"
     gene            6319..7446
                     /gene="dnaJ"
                     /locus_tag="PKM23_03585"
     CDS             6319..7446
                     /gene="dnaJ"
                     /locus_tag="PKM23_03585"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNY90588.1"
                     /translation="MASKDYYSVLGVSRDADADTIKKAYRKLAMEYHPDKNPGDKAAE
                     EKFKEIAEAYSILSDPQKRRQYDQFGSEGMRGGGAGGFDPRGFGDPFDIFREVFGGGF
                     GDIFGMGGGGGRRAAAQRGSDLQVRLKLDLEEIGRGVSKKIKIKKQVRCEKCGGTGAK
                     SGTSTTTCPQCQGRGEVAYRQGFFSISRTCGRCLGEGKVIEHPCSSCDGEGRVRGEST
                     LDVEIPAGVAEGQYLTIRSAGNIGLRGGPSGDVLVIIEEKPHPHFQRHGNDILYDLNI
                     SFPQLILGDEVEVPTLGGKARITIAAGTQSGKILRMRGKGIPNLNGGGAGDQLIRITA
                     YTPTKLGEAEKKMIRQLAEFESLYPEKADKGLFEKMREAIR"
     gene            7450..7890
                     /locus_tag="PKM23_03590"
     CDS             7450..7890
                     /locus_tag="PKM23_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNY90589.1"
                     /translation="MLEFSLQEKRFILFLLTTFLIGCAVTYYRQIHGDAELAMWRERQ
                     LALSREISPLTAIPGGAAAAPAAVAAPQSLLSRKLRLVSRLNLNTATAEELAALDRIG
                     PALAGRIIRYREEHGPFRTIDQLQEVKGIGPKTFARIRERIEVD"
     gene            7966..8907
                     /gene="pilM"
                     /locus_tag="PKM23_03595"
     CDS             7966..8907
                     /gene="pilM"
                     /locus_tag="PKM23_03595"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNY90590.1"
                     /translation="MDNTTTQLGLMMAKNRFYFLEAGTSRPLRLLSAGALDLDVPFDF
                     QSLADRELLSHFSGAISEMIDRFAISASAVSFCLDRRMVQLKRLKIDREFSEAEVRQQ
                     VEWELEQMLVSPRSEYHANYERVRSERDHYDHVIIAVVRKAIITYLNDIFKNTRVRLD
                     LVDVDLLAAIRGLLLLKPQSRGLAALIDVQEELLGITLIKDQGYLTYGELKYQSSELG
                     EENSEELATRINDETLRLMESLGDELLLKSIEEIHISAASLPTAVVHALQRLQRAAQI
                     QILEPLSQIDHSLSMEAEQMIRENARYILPLLGLLRA"
     gene            8959..9996
                     /locus_tag="PKM23_03600"
     CDS             8959..9996
                     /locus_tag="PKM23_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90591.1"
                     /translation="MNLLIDQREEAHMQEPEWTPETAEQPASPLMEDPALPRNAETPL
                     DYYNFDEPEGRKRSILGPVLIILLLLAAIGAAAYYGFFYKPEELGEFQKSAQQAAPQA
                     SEPAPVLTEPAAESTAPGGSVAEEISSAEAPATESPAPVQAESVQPAPARTLPAETGG
                     GSPLARAAGLLGGILAARPADLHVSTLILDQNSFSAEVSAQGRAVIENYANALKSSVP
                     GGLSSSPASGYYSGTRALVTGTFPSISSAAPPVVEAAELTRIKDDLQAAAREAGLKVI
                     EVSQAKPILRSGESWTTVFSKVSGSESQFQAYCLNLAGRLPQMRLDKIILQTDKAQKT
                     VGVLRLEARTR"
     gene            10000..10578
                     /gene="rsmD"
                     /locus_tag="PKM23_03605"
     CDS             10000..10578
                     /gene="rsmD"
                     /locus_tag="PKM23_03605"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNY90592.1"
                     /translation="MRVIAGRFRSRTIHSVKGEWLRPTADRTREFVFSWLGNLVEGAW
                     VLDLFAGTGSLGIEAISRGARAATFVDSSPAAYNLLRRNLESLGIEAALHRQEALTFL
                     KMAARLQQRYDLIFCDPPYRYQDIGLILSQVQSGQLLESKGLFVYEASSRDKFSCPEP
                     WRALKVKILGDTQIIFCGMSDDQENSDLSGLL"
     gene            10541..11029
                     /gene="coaD"
                     /locus_tag="PKM23_03610"
     CDS             10541..11029
                     /gene="coaD"
                     /locus_tag="PKM23_03610"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003144971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNY90593.1"
                     /translation="MTRKIAIYPGSFDPITYGHIDIIKRASLLFDEVIVAVARNLGKT
                     PLFSEEERVGMIEEAIRELPSVRVDSFNGLLVDYARSIGAQAVIRGLRAVSDFEYEFQ
                     MALVNRKLDEHMITVFLMPHEKYSYLNSSIVKELARFNGEVSCFVPPHVEKQLLEKMR
                     KK"
     gene            11026..12006
                     /gene="pta"
                     /locus_tag="PKM23_03615"
     CDS             11026..12006
                     /gene="pta"
                     /locus_tag="PKM23_03615"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNY90594.1"
                     /translation="MTILEQIQAKAKEKPQAIVFPEGEDDRTIQAAAICLANKLVSPV
                     LLGDKEKISSQAAALGVNISGAEIITPEKDARFEAYANTFYEMRKHKGLDPETARKNM
                     AQPLYFGAMMVREKRVAASVAGAAHTTGDVLRAAIQVIGVAPGFSVVSSCFLMVLPDG
                     RNLTFGDCAVIPDPTADQLADIALASAETHRSLTGEEPLVAMLSFSTKGSASHPFVDK
                     VQQAVAIARQKKPEIRIDGEMQADAALVSSVGQRKAPGSPVAGQANVLIFPDLQAGNI
                     GYKLVERLAGAQAIGPIIQGLAQPANDLSRGCKAEDIVNVACICSLKARN"
     gene            12025..13230
                     /locus_tag="PKM23_03620"
     CDS             12025..13230
                     /locus_tag="PKM23_03620"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNY90595.1"
                     /translation="MILAKRMVRMQESQTVALNSTLARLKREGKDVVALGAGEPDFDT
                     PGFIKEAAIEAIRQGYTKYTPAEGSMELREAIAAWLQAAYGARYTAKEIVVTSGAKFA
                     VFQAVLAVCDPGSEVVLPRPYWVSYPEMVQLADATCRFIDPADREHLKISAAELKAAI
                     SPKTRLVILNSPSNPSGAVYSREELAELVQVIRDTGVYLLSDEIYDQIVFDEGGYTSL
                     SQFPEIRSQLLLVNGVSKSCAMTGWRIGFLAAPEEIAKAVIKYQGHTTSNPASISQKA
                     ALAGYLGSKDFIGEMRAAFLERRDYVYKRLMAMPRISCLLPQGAFYAFPDVSAWFGRE
                     KNGFKITDSVSFCQYLLQEFGVAIVPGVAFGMDTHVRLSYATSMAVLEKALDRIESGI
                     KSLEQAGMS"
     gene            13230..13475
                     /locus_tag="PKM23_03625"
     CDS             13230..13475
                     /locus_tag="PKM23_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNY90596.1"
                     /translation="MPTYDYQCRNCGYIFEAFQSIAAEPLTVCPNCDQPDVHRILSGG
                     AGVIFKGSGFYQTDYKHSHSTTNSSTETKPEKKTNEP"
     gene            13465..14601
                     /locus_tag="PKM23_03630"
     CDS             13465..14601
                     /locus_tag="PKM23_03630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY90597.1"
                     /translation="MSRELAFDSSLRAGKRRLSISTRFEPESRKAVTSVQDGEELVDH
                     RENLIDESVAPEQLEAEVRQLHDLTVSDLDLLFCVNDKVLAGENAGSLTKLGTLFLEK
                     GFYDEAIDTFSTVLQRDENFENIHYSLGRAWYRKGDLDQALENLRRAAEKRPGYPDVH
                     YLLAEVLRKGGDHRAAAESCQKALSLNADYVSAHLLLGLIWAESTLLQPTHPEMAPPI
                     ERMKESKQHLLYAMDLVSPEQRAHLEKGLECLEIRERLEEGLAEIEKAIEPSVQSHRS
                     VIADSEFYLKFMFADLERDNRTLDSYIKTLEKALGQHPEYADLHQSLGTACLLRGWHS
                     FTRAVEAYREAVRINPGYEKAQKNLKLLENDGRGFLILLRAILK"
     gene            14932..15372
                     /locus_tag="PKM23_03635"
     CDS             14932..15372
                     /locus_tag="PKM23_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90598.1"
                     /translation="MKKSLFMVLLTGVLSLQAQSLHRVVASPFEPASPSVLRFEWDNG
                     GPITPLWRLGVVLPPEFDRTQLLLAASRSINGGFTVANSGDTLWAVRSGAGDRINAAE
                     RMDLSVASIGLPSDLQKSYPFIFIIQDSSRRALFSGTMTLSREE"
BASE COUNT         3180 a         4533 c         4590 g         3111 t
ORIGIN      
        1 ctggcggcct acggggagtt catccgcagc tatcccgact ggccggaaac ccccgacctc
       61 tatctgcgcg ctggcgacct ctacctcgac cgctttttca atctggcggc ggccgactcg
      121 gtttatcagc ggctgctgca gcgcccgaac ctgccggtct cctaccacct tctcgccctg
      181 cagaaacggg cccaatgcgc gatcgcgggc ggggatctgc gcagcgctga gaaatttatc
      241 tccacactgc acagggagac cccaacgggc tacggccggc agcgccaggc cgatctcatg
      301 ctcgtccaga tcgacctgtt tgaggggaga cccggccgcg ccctgaacaa gctggagaag
      361 ttgattggag gggccggagg tgcgggcgaa gccaaggccg acaccgtgca gaatgatctg
      421 ttggagcttt atctccttct gcgcagcagc cggcaggatt ctcttgaact ggtgcgctat
      481 ggcaaggccc tctggctgca gcgccagcgc agccatgctg ctgctttcga caccctggcg
      541 gctctgctgg ccggctacag gccgggtgcg ctggcggagc gggcgcgctt cctccaggtc
      601 gatctgctgc gcacgatggg gcgttttccc gaggccctgg tgatcagcgc cgcgctggcg
      661 gcggacagct ccggactggc cccggatttt gccctgatga ccatggccgg gcttcacgag
      721 gagttgaaag agccggaaaa ggcccggcaa ctttatgaaa gttttctgga aaaatatcca
      781 gaaagtatct atattgaaca ggtgaggggt cacatccgcc gtcttgaaac gcggagccgg
      841 tgatgccttc cgggaacggg gcagtaatgc gcagactggt atttttcatc gtgtggcttt
      901 tttcggccac ggccttcggg cagcagcttc tcgtctacat ggaccaccgg caggagaacc
      961 atctcaaggc ctacggcctc gcctactggg tgctggatca cggcgttgag gtggaatggc
     1021 tgctcaacta ccgcggcggc tcctttctga ccgcctattc aacggcgact gaaagggagc
     1081 tgcgggtgcg cggggtctcc ttttccacgc tctccggcgc agaggtgagc tccatctatg
     1141 ccgagatcga gcgcgaaaat atggaggttg tcaagctcga aaaggccccg gcggtggcga
     1201 tctatgcccc gcccaacaag caggcctggg acgatgctgt gctcatggcg ctcgattacg
     1261 ccgagatccc ctataccgtg ctctgggatg aagaggtcgt ccgcggcgag ctgcagaaat
     1321 acgactggct gcatcttcat cacgaggatt tcaccggtca gtatggcaag ttttatgcga
     1381 gctaccgcaa cgccgactgg tacaaggatg aggtgcgcaa gaatgaggct gtggcgcgta
     1441 aattgggctt tgccaaggtc tcggagttga agcgggcggt ggcccatggc atccgcgact
     1501 ttgtcgcggc cgggggtttt cttttcgcca tgtgttcggc caccgattcg ttcgaaatcg
     1561 ctttggccgc cagccgtgtc gatatcgtcg atgccctctt cgatggtgat cccatcgaac
     1621 cgggggcgca gggcaaactc gattatacac agactttcgc ctttgaaaat ttcaagatcg
     1681 agaccaaccc catggtctac gagtactccg acatcgatgt gcctccctcc tacgctccgg
     1741 tctcccgtgg tgctgaaggg gactatttca cactcttcga gttttccgcc aagtatgatc
     1801 ccgtgccgac gatgctcagc cagaatcatg tcgcagctat caagggcttc atggggcaga
     1861 cgacgggctt ccagaaaaag ttgatcaaaa agagcgtcac gatcatgggc gaagccgaag
     1921 gaactgacca ggtcaagtat ctgcgcggca atttcggacg cggtaccttt tgttatctcg
     1981 ccggccacga tccggaggat ttccagcatt ttgtcaatga tccccccacg cagctggcgc
     2041 tccataaaaa ctcgcccgga taccggctga tcctgaacaa cattctcttt ccggccgcgc
     2101 gcaagaagga gcttaagacc tgacctcctc catgctggtt gaggagccct ctcgggccgc
     2161 acggaatagc aattcagatg aggggtgatg ttcaaacaca gacatattcc gccggaacag
     2221 gtcattgcag cggaacccta tacggcgctg gcccagatct acgatcaggt gatgaatcat
     2281 gtggattatg accattgggc caggcatctg atccggctcg ctcggctgca cggggtggag
     2341 ccgcggacgg tgctcgatgt ctcgtgtgga accggcaccc tctgccgcga gtttcagtcc
     2401 cgcggattca gggttctggg ctgcgatgcc tcgctcccga tggtcaagat cgcggctgcc
     2461 tttcggagca gccgggcggc cattccctat tggtgcaccg cgatggaccg agtggcgata
     2521 cgaacgccgg tcgagctcgt cctttcctcc tacgacagca tgaactatct ccttgagccg
     2581 cagcagtggc agtctgcttt ccagaggctt cacgagctgc tcgtgccggg ggggctcttc
     2641 atttttgata tcagcaccct gcgcaattcg acggagacct ttgccgatta tgtccatcag
     2701 gaggagtttg cagagggatc ctaccggagg accagcctgt tcgacgccga aaggaatatt
     2761 cagtataatt atttcgagat agaattatcc agtgcacccg gctgtttata taaagaggtt
     2821 cattctcaat ccattcgcgc ccttgcgcaa gtcgaagact ttatcgccct gagccctttc
     2881 accttgttgg gccagtatgc caattttacc ttgcggcccg gcagtgagcg ggctgagcgg
     2941 gtccattttg tcctcaaaaa ggggcgcgaa acatgcatgt agcgggagca gcgagcatgg
     3001 ttgaactgca aagtgtgcgc gtcacctatg ccaacgggga gggcatcgat cagatcacct
     3061 tttcagtggc gccgggggag atggtctttc tggtgggtcc gagcggcgcc ggcaagtcca
     3121 cggttttgaa gactatatat atggacaaac agccggcgga ggggcacgtc gttgtcggcg
     3181 attacaactc tctttttatc cgcaagagcg aggttccctt tctgcggcgc aagctgggca
     3241 ttgtttttca ggatttccgt cttttcgctg accgggatgt tttcgacaat gtcgccttca
     3301 ctcttgaggt catcggcgcg cggcagaaag agatcaagcg gcgcgtgctg cgtgcccttg
     3361 ccgatgtcgg attgagccac aaggcgcgca agatgcccga cgagctctca gggggggaac
     3421 agcagcgggt cgcgatcgca cgggccatcg tcaacgaacc cctccttctg ctcgccgatg
     3481 agccgaccgg caatctcgac cccgaaacgg ccgcggggat catggatgtg ctgttgcgca
     3541 tcaacacccg tggtacctcg attttgatgg ccacacacaa ctatgatatg gtgcagcgct
     3601 atcgcggccg catcatcaag atcaacaacg gccgcctggt ggcctgagcc agtaacaagc
     3661 ggagtcgaat gaatctacgt catgttctga aagagacagc ggatggtttc aggcgcgcct
     3721 ggggctcctg cctcctctcc atcctgacga ttgcgttttt cctgtcgctg ttgggtttta
     3781 tcgccctggt ctccgccaac atcgatcatt tcaagcagac cctcaacgac cgtttgcagc
     3841 tgcaggtatt catctccggg accctggatg atcagcagat cgcggccctt tcacagcgca
     3901 tcagccggat ttcaggggtg gccaaagtga gcttcgtctc ccgggagatg gctgctgagg
     3961 agtttcagaa ggagttcgga cgcgagctgt tcgccgccct ggaggagaat cccctgccct
     4021 cctccttcgt cgtccgtctc ggcattacgg gagaggatga agggcgcatc aagcagatcg
     4081 ccgcccgcat cgaagaggag gcgggggtgg atgaggtggt ctatcatttt cagacccttt
     4141 tcaccctgca gcgctacgcc cggcttgccg cgaccacgag ctggatcctg ctgctctttg
     4201 tgacgctggg ttcgcttttt gtcgtctcca ataacatccg cctggtcatc agcgcgcgca
     4261 agcagatcat atcgacaatg cggctggtcg gggccacgcc gggattcatc cgctcgcccc
     4321 tgatcctgga aggggcgctg cagggggcgg ccggggccct tctggccggg ctggccctct
     4381 atttcgtcga ccgcctgctg gcccaactgc tgccgggact gctgcacttg ccgcctgaga
     4441 gcctgatcgt cctgctcctg ctcggctgtt ttctcggttt tcttggcagc ctgctggcga
     4501 tcaagcgcta cctgtgaaaa ggagccggca ggtgagattg gatgcacctg taaattttgc
     4561 ttgattgaac agttattcat tgttaattta gagaccgtta taaaatggtc tgatttttgc
     4621 gtgattgcct atgactctgg atgacttgac agaacgcgag cggcaggttt tcctctcgat
     4681 cgtgcagagc ttcatcgata cggctgaacc ggtcggttcg cgctatttgt ccaagttcgc
     4741 cgaattgaac ctgagtccgg cgaccatccg caatgtcatg gccgatctgg aagagaaggg
     4801 gctgatccgt cagccgcacg cctccgccgg acgtgtgccg acagacggcg gctatcgcgc
     4861 ctatgtcgat ggcatgatcg gcggggtcaa gctaagtccc tcggaacggc gatccatcgt
     4921 cgagcagctc aaccgcttcg ccgaggatgt agatcttatc atcgatcagg cctcgcatgt
     4981 gctcagcaac atttccagcc agctgggggt ggtgctggcc ccgcgcttca accagggccg
     5041 tctcgagaag atcgagctgg tttcggtttc agaagaaaag attctcgtca ttcttaccat
     5101 cgcctcgggc ctggtcaaaa ccattatcgt cgagatggat gaaaaggtgc ccggccatct
     5161 cctcgaggcg gccaggcaga tcctcaacga gcggctgcat ggcttgacca ttcaggagtt
     5221 ccagtccact ttcgaggaac gtttctcgga tctggatgag aacagcaagc gcgtgctcgg
     5281 caagatcagg agcagggctg aaaagctggt cgggtatgac ccggtggcct cctttcatgt
     5341 ggccggggcg ggcaagatca tcctgcagcc cgaattcgcc tcccaggaga aggtgggcgt
     5401 catcctgaac ctgatcgacc gccgcgacct cctgctgcgc gtgctcgagg agtcggactg
     5461 cagcggagtc tccatcgtca tcggcgatga gaacaagcag gaggtgatga agcactgcag
     5521 cattatcacc accacctatt ccatcggcgg ggccaccggc accatcggtg tgatcgggcc
     5581 gacgcggatg cagtatgcca agatcatcgg actggttcag tttatggcgg aaacgctcgg
     5641 atatgtttta aacaagcaga attagaaagg cagcaggttc ggagttgatg aaggaagaac
     5701 agaaaaacag cgacagggaa cagacggtag cggatctgga ggagtctctc caggaggagc
     5761 cgagagagca gtccggcccg gaagagcctt ctgcagatgg ggcaggggcc gaaaccgaaa
     5821 aggcggtgga cgctgacgcc gcggccatcg ccgccctgac ggctgaaaac ctgaagttga
     5881 aagaccagct gctgcggttg gcagcagagt tcgataacta ccgcaagcgc agcgaccgcg
     5941 atatgcagag ctatatcagt aatgccaatt acgacttgat ggcgaggctg ctgcccgctc
     6001 tcgatgatct ggatcgcatt gtcgccgccg ccggcggcgg cgctgaactg gccgccctgg
     6061 ttgatggcat caatctgctc cacaaaaaca tcctcaaggt ttttcaggat gggggattgg
     6121 aaccaatgca gaccatcggc gaggagttcg atcccctcct gcacgacgcc ctcctccata
     6181 ttgaggtctc cggcagcgag ccgaaccggg tggtcgaaga acacaagaag ggttatttct
     6241 tcaagggcag ggtcctgcgc catgcccagg tggtagtgag caagtaattt gatttttggg
     6301 acggaagcaa tatagcatat ggcaagcaag gactattact cagtactggg tgtgagccgg
     6361 gacgctgatg cggacaccat taaaaaggcc taccgcaaac tggccatgga gtatcatccc
     6421 gacaagaacc ccggcgacaa ggcggccgaa gaaaaattca aggagatcgc tgaagcctac
     6481 tccatcctct cggatccgca aaaacgaagg cagtacgacc agttcggcag tgaggggatg
     6541 cgcggaggcg gcgccggggg atttgatccc cgcgggttcg gcgatccctt tgatatattt
     6601 cgtgaggtgt tcggcggcgg attcggcgac attttcggca tgggcggagg cggcggccgc
     6661 cgcgcggcgg cccagcgcgg cagcgatctg caggttcgtc tcaaactgga tctggaagag
     6721 attggccgcg gcgtatctaa aaaaatcaag atcaaaaaac aggttcgctg cgaaaaatgc
     6781 ggcggcaccg gggccaagag cggcacctcg acgacgacct gcccgcagtg ccagggacgg
     6841 ggtgaagtgg cttatcgtca gggtttcttc tccatcagcc ggacctgcgg gcgctgcctg
     6901 ggcgaaggca aggtcatcga gcacccctgt tccagctgtg atggcgaggg ccgggtgcgc
     6961 ggcgagagca cactggacgt tgagatcccc gccggtgtcg ccgaaggcca gtatctgacc
     7021 atccgcagcg cgggcaacat cggcctgcgc ggcggaccca gcggggacgt actggtgatc
     7081 atcgaagaaa agccgcatcc ccatttccag cgccacggca atgatattct ctatgatctc
     7141 aacatcagtt ttccccagtt gatcctgggc gatgaggtcg aggtgcccac cctgggcggc
     7201 aaggcgcgca tcaccatcgc ggccggcacc cagagcggca agatcctcag gatgcgcggc
     7261 aagggcattc ccaatctcaa cgggggcggc gccggcgacc agctcatccg catcacggcc
     7321 tacaccccga ccaaattggg cgaggctgaa aagaaaatga tccgccagct ggcagaattc
     7381 gaatccctct atcctgaaaa ggcggacaag gggctgttcg aaaaaatgcg cgaggctatc
     7441 cgttagcgta tgctggaatt ttcactccag gaaaagcggt tcatcctctt tctgttgacc
     7501 acttttctca tcggctgcgc agtgacctat tatcgccaga tccatggaga tgcggagctg
     7561 gcgatgtggc gggagaggca gcttgctctc tccagggaaa tctctcccct gaccgcgatc
     7621 ccgggcggcg cagctgccgc tcctgcagcg gtggcggcac cgcagagcct gctcagccgc
     7681 aagctacgtc tggtctcccg gctgaatctc aataccgcca cagccgagga gttggccgcc
     7741 ctggacagga taggtccggc cctggccgga aggattatcc gctaccgtga ggagcatggg
     7801 cctttcagga cgatcgatca gctgcaagag gtcaagggga tcggacccaa gacatttgcc
     7861 agaatcaggg agagaataga ggttgactag cttacacacc aggcttcatg agcaaaatgg
     7921 ccgtattaat ctatagcgag agcagatctg gaggggcaaa acaacatgga taatacaact
     7981 actcagctcg gtctcatgat ggccaaaaac cgcttctatt tcctcgaagc cgggacaagc
     8041 cgtccgctga gactgctgtc agcgggtgct ctggacctgg atgtaccgtt tgattttcaa
     8101 tccctcgccg accgggaact tctctcccat ttttccggcg ccatctccga aatgatcgac
     8161 cgcttcgcca tctccgcctc tgcggtctcc ttttgcctcg accgccgcat ggtccagctc
     8221 aaacggctca agatcgatcg cgaattcagt gaagccgagg ttcgccagca ggtggagtgg
     8281 gagctggagc agatgctggt ctccccgcgc agcgagtatc acgccaatta cgagcgggtg
     8341 cgcagcgagc gggaccatta tgatcatgtc atcatcgccg tggtccgcaa ggccatcatc
     8401 acctatctca acgatatttt taagaacacg cgggtccggc tggatctggt cgatgtcgat
     8461 cttctcgccg ccattcgtgg cctgctgctc ttaaagccgc aatcccgagg cttggccgcc
     8521 ctcatcgatg tccaggaaga gctgctcggc atcaccctga tcaaggatca gggctacctc
     8581 acctatggcg agctgaaata ccagagcagt gagctcggcg aggagaacag cgaggagctg
     8641 gccacccgca tcaatgacga aaccctcaga ctgatggaaa gcctggggga tgaactcttg
     8701 ctcaagagca tcgaggagat ccatatctct gccgcaagcc tgccgacggc cgttgtgcac
     8761 gccctccagc gcttgcagcg cgccgcccag attcagattt tggaacccct gagccagatc
     8821 gaccattcgc tcagtatgga agcggaacag atgattcgtg agaacgcacg ctacatattg
     8881 ccgctgctgg gcctgctcag agcgtagtgc ctctggacgc ggcagtgatg accagttcta
     8941 cctagtaaag gggtcgatat gaatctattg atagaccagc gtgaagaagc ccatatgcag
     9001 gagccggagt ggacgccgga aacggcggaa cagcctgcct ccccgttgat ggaggatcca
     9061 gctttgccga gaaacgccga aacacctctc gactattaca attttgatga accggagggc
     9121 cggaaacgca gtattctcgg acctgttctg atcatcctcc tgctgctcgc cgccatcggt
     9181 gcagcggcct attacggctt tttctacaag ccggaggagc tgggcgagtt ccaaaaatcc
     9241 gcgcagcagg ctgctccgca ggcgtccgag ccggcgccgg tcctgactga accggctgcg
     9301 gaatccactg cccccggcgg atcggttgcg gaggagatct cctccgccga ggctccggcc
     9361 acagagtccc ccgcgccagt ccaggccgaa tctgtccagc ctgcgccggc caggaccctg
     9421 ccggccgaaa ccggcggcgg ctcgcccctg gccagggcgg caggactgct gggaggcatc
     9481 ctggccgcgc gcccggcaga cctgcacgtc agcaccctga ttctggacca gaactccttt
     9541 tccgcagagg tctcggcgca gggacgcgcc gtcatcgaga attacgccaa cgccctcaaa
     9601 agcagcgttc ccggggggct gagctcctcg ccggcgagcg gatactattc cggcacccgc
     9661 gccctggtca ccgggacctt tccctcaatc tcttctgctg cgcccccggt tgtggaggcc
     9721 gcggagctga cgcgcatcaa ggatgatctg caggccgccg cccgggaagc cggattgaag
     9781 gttatcgagg tctcccaggc caagcccata ctgcgcagcg gggaatcctg gacgacggtt
     9841 ttttccaagg tctcgggcag cgagagccag ttccaggcct actgtctgaa cctggccggc
     9901 cggctgccgc agatgcggct ggacaagatt atcctgcaga ccgacaaggc gcagaagacg
     9961 gtcggcgtcc tccgcctcga agcccgcacg cgttaacaga tgcgcgtcat cgccgggcgc
    10021 ttcaggagcc gcaccatcca ctccgtcaag ggagagtggc tgaggccgac ggctgatcgg
    10081 acccgggagt tcgtcttttc atggctgggc aatctggtgg agggggcctg ggttctcgac
    10141 cttttcgccg gcaccggcag tctcggtatc gaggccatca gccgcggcgc ccgggcagcg
    10201 acctttgtcg acagctcccc ggccgcctat aacctgctca ggcgcaacct ggagtccctc
    10261 ggcatcgaag cagcgctcca tcgccaggag gccctgactt ttttaaagat ggcggccagg
    10321 ctgcagcagc gatacgacct catcttttgc gacccgccct accggtacca ggatatcggc
    10381 ctgatcctct cccaggtgca atccggacag ctcctggagt caaaggggct ctttgtctac
    10441 gaagcgagtt cgcgcgataa attcagctgc ccggaaccct ggcgtgcgct gaaggtaaaa
    10501 atattgggcg acacccagat catcttttgc gggatgagtg atgaccagga aaatagcgat
    10561 ctatccgggc tcctttgacc cgatcaccta cggccatatc gacatcatca aacgggccag
    10621 cctccttttc gatgaggtga tcgtcgctgt ggcgcgcaat ctcggcaaga cgcctctctt
    10681 cagcgaggag gaacgagtcg gcatgatcga agaggctatt cgtgaattgc cctccgtcag
    10741 ggtcgacagc ttcaatggac tgctggtcga ctatgcccgc agcatcggcg cgcaggcggt
    10801 catccgcgga ctgcgcgccg tctccgattt tgaatatgag tttcagatgg ccctggtcaa
    10861 ccgcaagctc gacgagcaca tgatcacggt ctttttgatg ccgcatgaaa agtattccta
    10921 tttgaactca agcattgtca aagaactagc ccgttttaac ggggaggtga gctgttttgt
    10981 gccgccccat gtggaaaaac aactgctgga aaagatgagg aaaaaatgac gattcttgaa
    11041 cagatccagg ccaaggcaaa ggaaaagccc caggcgatcg tttttccgga gggtgaggac
    11101 gaccgtacca tccaggcggc agccatctgc ctcgccaaca agctggtctc cccggtgctc
    11161 ctcggcgaca aggaaaaaat ttcatctcag gccgctgcgc ttggggtcaa tatctccggc
    11221 gcggagatca taacaccgga gaaggatgcc cgttttgagg cctacgccaa taccttttat
    11281 gagatgcgca agcacaaggg gctcgatccg gagaccgccc gcaagaacat ggcccagccc
    11341 ctctacttcg gggcgatgat ggtgcgcgag aaacgggtgg ccgccagtgt ggcgggtgcc
    11401 gcccatacca ccggcgacgt gctccgggcc gccatccagg tcatcggggt cgcccccggt
    11461 ttttccgtgg tctcgagctg ttttctcatg gtccttcccg acggccgcaa cctcaccttc
    11521 ggcgactgcg ccgtgattcc ggatcccacc gccgatcagc tggcggacat cgcgctcgcc
    11581 tctgcggaga cgcatcgcag cctgaccggc gaagagcccc tggtggccat gctctccttt
    11641 tcgaccaagg gcagcgcctc gcaccccttt gtcgacaagg ttcagcaggc ggtggccatt
    11701 gcccggcaga aaaagccgga gatcaggatc gatggcgaga tgcaggccga tgccgccctg
    11761 gtctcttcag tcggtcagcg caaggccccg ggcagccccg tcgccggaca ggccaacgtc
    11821 ctgatctttc cggatctgca ggccggcaat atcggctaca agctggtcga acggctggcc
    11881 ggcgcccagg ccatcgggcc gattatccag ggattggcac agccggcgaa cgacctgtcg
    11941 cgcggctgca aggctgagga tatcgtcaat gtcgcctgca tctgcagcct caaagccaga
    12001 aactagccgg agagagagga gattatgatc ctggccaaac gcatggtccg gatgcaggaa
    12061 tcgcagaccg tcgccctgaa cagcaccctg gccagattga agcgggaagg aaaggatgtc
    12121 gtcgccctcg gcgccgggga gcccgatttc gacacgcccg gttttatcaa ggaggcggcg
    12181 atcgaggcca tccgccaggg ttataccaag tacaccccgg ctgagggaag tatggagctg
    12241 cgcgaagcga tcgcggcatg gctgcaggcg gcctacggcg cccgctatac tgcaaaagag
    12301 atcgtagtca cctccggggc gaaattcgcc gtttttcagg ctgtgcttgc cgtttgcgat
    12361 cccggcagtg aggtggtcct gccgcggccc tactgggtga gctatccgga gatggtccag
    12421 ctcgccgatg ccacctgccg cttcatcgat cctgcggacc gggagcatct caagatttcg
    12481 gcggcggaat tgaaggcggc catcagccca aagacccggc tggttatcct caacagcccg
    12541 agcaacccct ctggcgcagt ctacagccgc gaagagctgg cggaactggt tcaggtcatc
    12601 cgcgacaccg gggtctacct gctctcggat gagatctatg atcaaatcgt cttcgatgaa
    12661 gggggttata ccagcctgag ccaattcccg gaaatccgca gccagcttct gctggtcaac
    12721 ggcgtctcca aaagctgcgc catgaccggg tggcggatcg gttttctcgc cgcgccggag
    12781 gagattgcca aagcggtgat caaataccag gggcacacta cctccaatcc cgcctccatc
    12841 tcccaaaagg cggcgctggc gggctatctg ggttcaaagg acttcatcgg ggagatgcga
    12901 gcggcctttc tcgagcgccg ggattatgtg tacaagcggc tgatggcgat gccccggatc
    12961 tcctgcttgc tgccgcaggg ggccttttac gcttttccgg atgtctccgc ctggtttggc
    13021 cgggaaaaga atggtttcaa aattaccgat tcggtgagtt tctgccagta ccttcttcag
    13081 gaattcgggg ttgcgatagt gccgggagtg gcctttggca tggacaccca tgtccggctc
    13141 tcctatgcga cttccatggc agttctggaa aaggccctcg atcggattga aagtggaata
    13201 aagtcgcttg aacaagcagg gatgtcttga tgccaaccta tgattaccag tgcaggaact
    13261 gcggatatat ttttgaagcc tttcagagca tagcagccga accccttacc gtctgtccga
    13321 actgtgacca gcccgatgtc catcgcatac tcagcggcgg ggccggagtg attttcaagg
    13381 gttccggttt ttatcagacc gattacaagc acagccattc gacgaccaac tcttccaccg
    13441 agaccaagcc ggagaagaaa accaatgagc cgtgaactgg cattcgacag ctcgctgcgg
    13501 gcgggcaaac gcaggctttc gattagcacc cgtttcgagc cggagtcgcg caaggcggtg
    13561 acctccgtcc aggacggcga ggagcttgtg gatcaccgtg aaaacctgat cgatgagagc
    13621 gtcgcccctg agcagctcga ggccgaggtc cgtcagttac atgacctgac tgtgtcggat
    13681 ctggatctgc tcttttgcgt gaatgacaag gtcctggccg gagaaaatgc cggatcgctg
    13741 accaagctgg gcaccctttt cctcgaaaag ggtttttacg acgaagccat cgacaccttt
    13801 tccaccgtgc tgcagcggga tgagaatttc gagaacatcc attacagcct gggacgggcg
    13861 tggtaccgga agggtgatct ggaccaggcc ctggaaaacc tgcgcagggc ggccgagaag
    13921 cggcccggat atcccgatgt ccattatctc ctggccgagg tgctgcgcaa gggcggcgat
    13981 catcgcgcgg ccgccgagag ctgtcagaag gccctctcgc tcaatgccga ttatgtcagc
    14041 gcccatctcc tgctcggcct gatctgggcg gaatcgaccc tgctgcagcc gacccatccc
    14101 gagatggccc ccccgatcga gcgtatgaag gagagcaaac agcatctgct ttatgcaatg
    14161 gatctggtca gccctgaaca acgtgcacat ctggaaaagg gactggaatg cctggagatc
    14221 cgggagcgtc tcgaagaggg gttggccgag atcgaaaagg cgatcgagcc ctctgtgcag
    14281 agccaccgca gcgtcattgc ggacagcgag ttctatctga aattcatgtt cgccgatctg
    14341 gagcgggata accgcactct cgacagctat atcaagaccc tggaaaaggc cctcggtcag
    14401 catccggagt acgcagatct gcaccagagc ctgggcactg cctgcctttt gcgcggttgg
    14461 cacagcttta cccgggcggt cgaggcctat cgcgaggccg tccgcatcaa tccgggctac
    14521 gagaaagcgc aaaagaacct caaactgctg gaaaacgacg ggcgcgggtt tttgattctc
    14581 ctgcgcgcca ttctcaaatg acttacggtc atccgcacac ttcttcagac tgcgtcaaca
    14641 ccggtgtatc catacgacca ggcatcgatc aaggttcttc cgtgatagga aaggcatcaa
    14701 cacgggtgaa ggcggagttc cagcctgata ccctgcccat tgttacgacg atctaaccct
    14761 tttgctggta taatgtttgc aactgtctac aggcacatta aaagcggccg caaagtgccc
    14821 ggttttcgcc ctttattgtt tcgaagcagg acaggggaaa ccaggtgaga cacccggccg
    14881 tgcagcgtgc gccctggtag catctcaaac ggaaatgtca tgattgctca aatgaaaaag
    14941 agtctgttca tggttctcct gaccggcgtc ctctccctgc aggcccagtc cctccatcgg
    15001 gtggtcgcct ctcccttcga acccgcttct ccgtctgtgc tccggttcga atgggataat
    15061 ggtggaccca tcacacccct gtggagattg ggggttgtgc tgcctcccga attcgaccgc
    15121 acccagcttc tgctcgcggc ctcgcggagc attaacggcg gatttacagt ggccaacagc
    15181 ggcgacaccc tgtgggccgt gcgcagcggc gccggggacc ggatcaacgc cgcagaacgg
    15241 atggatctct ccgtcgccag catcggcctg ccctcggatc tgcaaaagag ctaccccttt
    15301 attttcatca ttcaggacag cagccgtcgc gccctgtttt ccggcaccat gaccctttcc
    15361 cgtgaagagt gagatgcagc agacaatgtc aggcaaatcc gtggccaagc gatt
//
LOCUS       DAOPWU010000037        15413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3902_length_15413_cov_4.436841, whole genome shotgun sequence.
ACCESSION   DAOPWU010000037 DAOPWU010000000
VERSION     DAOPWU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15413
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3902_length_15413_cov_4.436841"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3802
                     /locus_tag="PKM23_03640"
     CDS             <1..3802
                     /locus_tag="PKM23_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNY90599.1"
                     /translation="GRLAVAPEPFHWVNSQAAEVVIGQADFSTSASGLSASQFNTPQA
                     ICYHPPSGKLFISDRLNNRVLRFSSAAALTSGSSAEAVLGQPDFTSKTATVSAAGMSA
                     PIGLAIEEDGTLWVGDFSNKRILRFDDAVNLPSGAAASGVLGKADFTTSSGTVSQSVI
                     GGPVGISIHPDGTLWVADFSAQRILRYDDAKHKPNGAPADGVLGQSDFSSTGGRTTAD
                     GLNGPNNIYADLDGTVWACEFTNNRVVRYDDAKNKPNGAPADGVLGQPDFTSKTAATT
                     QTGMRDMRAVFGDGSGQIYTAEDGNNRVLVFNNAATLANGAPADFVLGQPDFITRTGL
                     TPPTAASLRQPRGIAIDTQNRRVWITDSQNNRVLMYTEKPYTPPDKPVYATHPLDIRL
                     FTEGRDDAYLTESWGYATAPSLIERSSGENRIPVDTTEAYSDPASLRLTWNSKAGGDW
                     AMGIFFPGLIRMDVSWKDSLSFLIYSGTPLSPTTLPHLYMSDNTGYNSPRISIAAWVD
                     TVKAGHWTRVTVPLQLFFASGSDLDAWSVRSLFFAQGEADEQDHTLLIDEIRFTGGND
                     FAGKRVVVVLGGEIPEGMGASTADSSWVGRYRAQLTAASDKFAVVNLGVGYNTTYHML
                     PDGSPLPPGNPFYPWENNNISFAAAFKPEAVIINLSFEDARAGFTTAEQLANLDSITA
                     YASRNKLNLWLTTTMPRAGIEAVEKQNLIEMRDAILARYPQVINLWPLLADAQDDLDA
                     LYRSTSGWNLNDAGHAALFEAMLTAEIVQLPGPKPVYTFDALDLKLYTDARTGTYWDA
                     SWGYVNAPSALELYNGDKYPLSSDYLYNGSYNLKLRWTSKSGGDWAVAVASEGWHKMD
                     ATWKDSLSFLVYSPRLISAAALPKIFLEDIGSVKTDRFALADFHGDIPAHTWTRIVVP
                     LKPFFDHPGQAKLSQMKTLFLSQNGADGEEHLLYIDEVRLTGGKTFTDKQVVVVIGSS
                     TAEGTGASPADSAWVNRYRSYLQKYSSDFEVINLAIGGFTSYRVMPTATTLPSNRTDY
                     PRENNNITYALAFHPKAIIVNLPTNDAANYYTISEQVANFDTLAAYAGRFQIPVWFST
                     TQPRNFSDAGQRANLVTMRDSIISRYSNRQWTRVIDFWTGLADANGNIVSTYNSGDGV
                     HLNNKGHRLLYERVVEARVLTYTGVARPAEALPEAYQLYHNYPNPFNPATTIRYDLPR
                     ESRIRLAVYDLTGREVALLHDGMQQAGRHEVEWNAAPFASGVYMIRMQTEGWSQVSKM
                     MLIK"
     gene            4044..5225
                     /locus_tag="PKM23_03645"
     CDS             4044..5225
                     /locus_tag="PKM23_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNY90600.1"
                     /translation="MGNIAEINRRDLLKLGAGVGLLLGNPALWAQEAQKPQPPARPKT
                     NVDEALKIPRGAHSLPGPFPGRVVQVSDAAVTAEKRVQPEVVNAMFEKGLQKLTGAGM
                     KKSFKLLFDKKDIVGIKVNPVGPGVIATHLEVVDAVIAWLVKNGLKRDRIIIWDRFDY
                     MLRDAGYTAERFPGIAIEGLQTMDEAAAEGKADNSRWLKPDGTHVSAGNFDQQVYYWA
                     DVEGDKEEAYLNQHVFNGRHSYFGRLLTQRLTKIINIPVFKNTGNGVSMATKNLGYAA
                     ICNTARLHRPLFFDVCTEVLAFPVIRDKLVLNITDGLRGQYDGGPDGAAQFTWDYNSL
                     FFATDPFALDMVCHNLLLAKRKEMQVKVNEHPRYTEYLRYAEKLGLGIAAPEKISHVV
                     V"
     gene            5212..6153
                     /locus_tag="PKM23_03650"
     CDS             5212..6153
                     /locus_tag="PKM23_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90601.1"
                     /translation="MSWSEPHLLAAAAPLAIMTLRAAALRLLRTVRSGMAGVLILMSF
                     ALSSAAAADLPGTGFYPGWSPAGKPRIYEAKNLYGYIDGGAELFLEFGFSGLTVQRYS
                     RGEEELSLDLYEMTSADAALAIYLAKKGEEKPVAGLSCRSTGSAWQIAALRGRHFIQL
                     TNLSGEESLLPAMVALLQALLPALPDEPARDWLTAMPAERIPGSELLLAGPWSLQMIY
                     TLGDGDLLQLGGRVAGVAVEQPGKDGKREACLRLFYPDSSAARAAFAHTTAHLDPYLT
                     PLHAAGDTLLFRDYKEEYGRIVRCDSVIQIELHLSQP"
     gene            6174..8480
                     /locus_tag="PKM23_03655"
     CDS             6174..8480
                     /locus_tag="PKM23_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNY90602.1"
                     /translation="MRRKTILLALLMLLSSSALLRAGTEIIAHRGASGLAPENTLASF
                     ARAVEIGADWFELDVWLSKDDSLMLMHDESVARTTGAGGTIPTMTYDQLRSLDAGSWF
                     SPLFAGEKIPTLAEALDLALRASYRVGVVIEIKSNAATVVDKVIAAVQQRGMQGRVII
                     SSFNSSHISKSKQLDPSIQVQLFGSITLAQIRQVDGIGGEWVGTNGAITAALLDSAHA
                     RGMKMNKWTINSASEMLTLIKQGVDAITTNFPDIARALVDTTPPGEVVLLPPVVDETR
                     IGLSWLPAEDPESTSITYEIYRGTQAHPEILLATVTDTTAYIDETRRESQQYFYRIRA
                     KNVAGLYSPAWSNEVQATTAPDLRPPQVTAVRAYGRPDHLVISFNERVDGESARAAAN
                     YQIGPGVTVHSARLALDSLSVMLDTTPLADKSSYTLTITGVKDQAIHPNPIIEPLPVP
                     FTYMPYRPGLVAAWDFDQEDGNNLPDHSGNANHGALQNGIAWSGGHTGNGLLFDGVDD
                     YILVPASSSLDINTQGITLSLWTWMAYAPNDLPGAYGPLYDSESDTYVLYEDKGIHEL
                     RFKVTTTVSAERPGIRGSELSAGEWVHLVGLYDGANAMIYLNGELKDTHPLTGTVKAG
                     QIATIGKTGGAYFKGRIDDIQIYNRALSAEEILFLYDGREITSSASRDPHQPEEFGLS
                     QNYPNPFNPSTTLNYRLSRRELVDLAVYDLLGRRIADLVHELQGPGQYQATWDGLDKN
                     GLPAGSGLYFCKLKSGGRTITRAMTLIK"
     gene            8755..9543
                     /locus_tag="PKM23_03660"
     CDS             8755..9543
                     /locus_tag="PKM23_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNY90603.1"
                     /translation="MARGTSGTVLLAEERRNRIEEILKVQNRIFVAEMARQMKTSEVT
                     IRKDLDVLERRGVLKRVHRGAILVSNPTVIDLDLVEKEHIHSREKDKIARKAADLIRD
                     GEAIILDSGSTTTAIARALKARSGLTIITNAINIAWELTASKNQVILIGGMVRENSFS
                     LVGPLSEEAMRNLSADKLFLGVDGIHFEFGLTTPNLQEATINRMMLHGATDVFVVADS
                     SKIGRRSLGVIAEVTAAHHLITDDHITPADREKLRELGIDVIIA"
     gene            9606..10361
                     /locus_tag="PKM23_03665"
     CDS             9606..10361
                     /locus_tag="PKM23_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90604.1"
                     /translation="MQTGLTLLFLLSSFTAALASSRLPIDSSSDYLCYYGSWDNDKLF
                     RAKDFDLVILEPSNISAAQIAEMKKGHDGLAGTPDDVIVIGYVSIGEDHQGGRTGDGR
                     GPCYWDWGSSSVVYTNGGYASWYLDDANHDKKPDMNGSWGSYYVNAGDSSWRAFLKSS
                     PTGTDNILRTKNCDGLFLDTIDTASPWSPWPYRWMVVAMSDMVGWLHTIYPDKYLVQP
                     RALLFRPRSRHCLRPLDPPLYRRGDVRILLQGG"
     gene            10333..11745
                     /locus_tag="PKM23_03670"
     CDS             10333..11745
                     /locus_tag="PKM23_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90605.1"
                     /translation="MFESYYKEGERAVWAAKVNAEAAKPDGFAVIALDYYSASQTAAI
                     DKQVAEVLGLGWSDYISSTSLNEIRYEAFHRHPVDVNPPTWKSSIGLIEAATSDGAVK
                     LRWRQVTDQSAPVSFTVYYSSAPLFSIEGATRLEQVPATWQSSSLSWEYQLTGLANFT
                     TYTFLVRARDALGNEDKNLRLLKATPPAGMNTLLTIDGLFNDWGGIAPLDTPPNPVEA
                     GGYGLDAAADIVDIYAAHDAANFYLQFTTAAAFGSGYFYHVFFDIDNNPATGYLYGDS
                     ASTGSELMMENLALWQYTGTGGSNWSWSQAAGLTRATSGSQTELSIPLSLIPAAVQQG
                     KITLLLQTNRAVSPFTFVDAAPDRFGSERLVYSFNSGTAVPVEPAAARPGGLILGQNY
                     PNPFNSTTRIPFALDTPGEVSIRVFNLRGESAARYDLGMKSAGSHQFTLSAQELTSGI
                     YYYELLTGGKRQTGKMILIR"
     gene            11842..13563
                     /locus_tag="PKM23_03675"
     CDS             11842..13563
                     /locus_tag="PKM23_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90606.1"
                     /translation="MKKELLICLALVGMLLSGQPAVAQISIDGLFFDWEAATQVDVAP
                     QAEELTFDLGDPDCPNPAVPSYFADLDIEDLFVTDDADFVYFRIKMNPVANLTNVPND
                     TSYHGGAALAAYISVDPGWADITGLTWGWWGSGYDYFVQVYPADSAAFAATGYEQYVW
                     EHKQAGNGWDFIPADSTIGSRVAWNATSNEVEFSVPKSLLLNPRYIPDFVSPDTIAVM
                     IYAGENLAPWRADYASNPGIAGFKFALKQPGPIAIDGLFFDWTAEMQLDTPATSEELT
                     FDAGDPDCPNPALPSYFADLDIDDVYATDDADFIYLRIKMNSIANLTNVPNDTSYHGG
                     AAVAAYISVDPGAADTTGVTWGWWGNGYDFFVQAYPPDSAYAAVGGYPQGVWEHKQAG
                     NGWDFEVVDSTRGAWVAWNALNNDVEMAIPKAQLLNPRYLQNFQQPDQIAIMIYAGEN
                     LAPWRADYASMPGVAGFLLNLGGSTGVATRPAGRIPVNMALEQNYPNPFNPETRIRFA
                     LPAQGSVRIRVFDLLGKEVASLADGVFSAGEHVVTFSGKDLPSGVYFYALEAGGSREI
                     RKMMLIK"
     gene            13682..>15413
                     /locus_tag="PKM23_03680"
     CDS             13682..>15413
                     /locus_tag="PKM23_03680"
                     /inference="COORDINATES: protein motif:HMM:NF027642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4957 domain-containing protein"
                     /protein_id="HNY90607.1"
                     /translation="MRITLRFFLFFLLSFSLAAFAQTVHYVEAGPGAISAAYQAAAAG
                     DIIELVTSGGTYTESATMEITADKAITIRAAAGITSPVWQCEASPFMTVGGTLIMEGL
                     TLDGQEAAKRAINTTGSAGYSIKIVNSTLQGFEYMIYGSSSSALDSLVILNSLFKKAT
                     NRALYFPAGTIDPGVVKRLSIRNSTFMEMKKTEPSIYFYAKSSPTVSPRLELDHVTFY
                     NCQRIRTNSGVTDVSIRNTIFATDGTISGGQSFNLNGGVVENTLVFNAPVSGSGASYS
                     GFMDNVDPLFTDAANGDLTLLEATPAFHAGSDGKTLGDPRWWPRLSGRIYVAAGRDQI
                     AAAVAAANPGDVIELTSDGGLYVESSTVVIDKPVTIQPSESIVNPPVWTSDDGGYLIL
                     TRAGLTLTGVVLDGGKGALQAAGGIATDSSGYNLILTGCDFLNFGGESSTTGHAIYDG
                     LYQGEVDSLVISSCYFNSIAREGIYLGGRTAAQIGTVKYFHMVNSTMARIGDDAVYIR
                     DHDGNFSTPGPVYLIDHCTFYDAFDSYGLLAHYIDNALIKNTIAAASTVKGTAFYLYG
                     DNSLVKNSLYF"
BASE COUNT         3110 a         5356 c         4097 g         2850 t
ORIGIN      
        1 cggccgcctg gctgttgccc cggagccctt ccactgggtc aacagccagg cggccgaggt
       61 ggtgatcggc caggccgatt tttccacctc tgcctccggc ctctccgcct cacagttcaa
      121 tacaccccag gccatctgct atcatccgcc ctccggcaag ctcttcatca gcgaccggct
      181 caacaaccgc gtcctgcgct tcagttcggc ggcggcactc acgagcgggt cgagcgccga
      241 agcggtcctt ggccagcccg atttcaccag caagaccgcg acggtcagcg ccgccggcat
      301 gagcgccccc atcggactgg cgatcgagga ggatggcacc ctctgggtcg gcgatttttc
      361 caacaaacgg attttgcgtt ttgacgatgc cgtcaacctc cccagcggcg ctgctgccag
      421 cggcgtcctc ggcaaggccg atttcaccac cagcagcggc acagtctccc agagcgtcat
      481 aggcggtccg gtcggtatct cgattcatcc cgacggcacc ctctgggtgg ccgatttcag
      541 cgcccagcgc atcctgcgct acgacgacgc caaacacaag cccaacggcg caccggccga
      601 cggcgtgctc ggccaatccg atttctcctc caccggcggc cgtaccaccg ccgacggcct
      661 gaatggcccg aataatatct atgccgatct cgacggcacc gtctgggcct gcgagttcac
      721 caacaaccgc gtcgtgcgct acgatgatgc caaaaacaag cccaatggcg cgccggcaga
      781 cggcgtcctc ggccagcccg atttcaccag caaaaccgcc gccaccacgc aaaccggcat
      841 gcgcgacatg cgcgccgtct tcggcgatgg cagcggccag atctataccg ctgaggatgg
      901 caacaaccgc gtcctcgtct tcaacaatgc cgccacgctg gccaacggcg ctccggccga
      961 tttcgtgctc ggccagcccg attttatcac tcgcaccggt ctgacccccc cgactgccgc
     1021 cagcctgcgc cagccgcgcg gtatcgccat cgacacccaa aaccgccgcg tctggatcac
     1081 cgactcccag aacaaccgcg tgctgatgta cacggaaaaa ccctataccc cgccagacaa
     1141 gccggtctat gccacccatc ccctcgacat ccggctcttc acagagggcc gcgatgatgc
     1201 ctacctgacc gagagctggg gttacgccac cgccccgagc ctgatcgaac gctcctctgg
     1261 ggagaacaga attccggtcg atacgacgga ggcctactcg gatccagcca gcctgcgcct
     1321 gacctggaac tcgaaggccg gcggcgactg ggccatgggc atcttcttcc cgggtctgat
     1381 caggatggac gtgagctgga aggacagcct ctcctttctg atctactcag ggacgcctct
     1441 ttcccccacg acactcccgc acctttatat gtcggataat accggctaca attcaccgcg
     1501 catctccatt gccgcctggg tcgacaccgt gaaggcgggg cactggacgc gcgtcaccgt
     1561 ccccttgcag ctcttcttcg ccagcggcag cgacctcgac gcctggagcg tgagaagcct
     1621 cttcttcgcc cagggcgagg ccgacgagca agaccacacc ctgctcatcg atgagatccg
     1681 ctttaccggc ggcaatgatt ttgccggcaa acgcgtcgtg gtcgtcctcg gcggcgagat
     1741 ccccgagggc atgggcgcca gcaccgccga cagcagctgg gtgggccgct accgcgcgca
     1801 gctcaccgcc gccagcgaca aattcgccgt cgtcaatctc ggcgtcggct acaacacgac
     1861 ctatcatatg ctgcccgacg gctcccccct tcctcccggc aatcctttct atccgtggga
     1921 gaacaacaat atctccttcg ccgccgcctt taaaccggag gcggtgatca tcaacctctc
     1981 cttcgaggat gcccgggcgg gattcaccac cgcggaacag ctggccaatc tcgactccat
     2041 caccgcatac gccagccgca acaaactgaa cctgtggctc accaccacca tgccccgcgc
     2101 cgggattgag gccgtcgaaa aacagaatct catcgagatg cgcgatgcca tcctggcccg
     2161 ctatccccag gtcatcaatc tctggcccct gctcgccgat gcccaggatg acctcgatgc
     2221 tctctaccgc tccaccagcg gctggaatct caacgacgca ggccatgcgg ccctctttga
     2281 ggccatgctc actgccgaaa tcgttcaact gcccggtccc aaaccggtct acaccttcga
     2341 tgcgctcgat ctcaagctct ataccgacgc ccgcaccggc acctactggg acgccagctg
     2401 gggatacgtc aacgccccga gcgcgctcga actttacaac ggcgacaagt atcccctctc
     2461 cagcgactat ctttacaacg gcagctacaa cctcaaactg cgctggacct ccaagagcgg
     2521 cggtgactgg gccgttgcag tggccagcga aggctggcac aagatggatg ccacctggaa
     2581 ggacagccta tctttcctgg tctacagccc ccggctcatc tccgcagccg ccttgcccaa
     2641 aattttcctc gaggacatcg gcagcgtcaa gaccgaccgc tttgccctcg ccgattttca
     2701 cggcgatatc cccgcccata cctggacacg gatcgtcgtg cccctcaaac ccttcttcga
     2761 ccatcccggc caggccaagc tctctcagat gaaaaccctc ttcctcagcc agaatggagc
     2821 ggacggcgag gaacatctcc tctatatcga tgaagtgcgc ctgaccggcg gcaagacctt
     2881 tacggacaaa caggtcgtgg tcgtgatcgg ctcctccacg gccgaaggga ccggcgccag
     2941 tccggccgac agcgcctggg tcaatcgcta ccgcagctat ttgcagaaat acagctccga
     3001 ctttgaggtg atcaatctcg ccatcggcgg cttcacttcc taccgggtca tgccgaccgc
     3061 aaccacgctg ccgtcgaacc ggaccgatta cccacgcgag aacaacaaca tcacctacgc
     3121 cctcgccttc catcccaagg cgatcatcgt caacctgccc actaacgatg cggccaacta
     3181 ttacaccatc tccgaacagg tggccaactt tgacaccctg gcggcctatg ccggaaggtt
     3241 ccagattcca gtctggttct cgactaccca accgcgcaat ttcagcgatg ccgggcagcg
     3301 tgcgaacctg gtgacgatgc gcgattcgat tatctcacgc tactccaaca ggcagtggac
     3361 ccgcgtcatc gatttctgga ccggactggc ggacgccaac ggcaacatcg tctccaccta
     3421 caactccgga gatggtgtcc atctcaacaa caagggccat cgcctgctct acgaacgggt
     3481 ggtcgaggcg cgcgtgctca cctataccgg cgtcgcccgg cccgccgagg cgctgcccga
     3541 ggcctatcag ctctatcaca actatcccaa tcccttcaat ccggccacga ccatccgcta
     3601 cgatctgccc agggagagcc gcatccgcct ggctgtctat gacctcaccg gacgcgaggt
     3661 ggccctcctc catgacggta tgcagcaggc cggccgccac gaggtggagt ggaacgccgc
     3721 ccccttcgcc agcggtgtgt acatgatcag gatgcagacg gaggggtgga gccaggtcag
     3781 caagatgatg ctgatcaaat aggcaggagc cggctccgcc tgagcacagc tggaaggacc
     3841 agcaaagagg cggagaaggc gaaagcctga tgccagcact tcatgaccag ccgggcccgc
     3901 gggcccggct ttttttatgc ccgcctggaa catgcgctat ttccccctca gggcgatttc
     3961 tctttggatt agtgacgatt tttttgtaat atcacgcatg atcgcatgaa aggagagcta
     4021 tgcggaaaac aaaatacaga tcgatgggaa acatcgcgga gatcaaccgc cgcgacctgc
     4081 tcaaactggg ggcgggagtg ggactcctgc tcggcaatcc tgcgctctgg gcccaggagg
     4141 cgcaaaaacc gcagcccccg gccaggccga aaacaaacgt cgatgaggcg ctgaaaatcc
     4201 ccaggggagc ccactcgctg cccggccctt tccccggtcg ggtggtgcag gtgagcgatg
     4261 cggccgtcac cgctgaaaaa cgcgtccagc cggaggtggt caacgccatg ttcgaaaaag
     4321 gtctgcaaaa actgaccggc gccggcatga aaaagtcctt caagctcctc tttgacaaaa
     4381 aggatatcgt cggcatcaag gtcaatccgg tcggcccggg tgtcatcgcc acccatctcg
     4441 aggtggtcga cgcggtcatc gcctggctgg taaaaaacgg cctcaaacgc gatcgcatca
     4501 tcatctggga ccgcttcgat tatatgctca gggatgccgg ctacaccgcg gagcgctttc
     4561 ccggaatcgc catcgagggg ctgcagacca tggacgaggc cgccgccgag ggcaaggccg
     4621 acaacagccg ctggctcaaa cccgatggaa cccatgtcag cgccggcaac tttgaccagc
     4681 aggtctatta ctgggccgac gtcgagggcg acaaggagga ggcctatctc aaccagcacg
     4741 tcttcaacgg cagacactcc tatttcggcc gcctgctcac gcagagactg accaaaatca
     4801 tcaacatccc ggtcttcaag aacaccggca acggcgtctc catggcgacc aaaaacctcg
     4861 gctacgccgc catctgcaac accgcacgcc tccaccgtcc cctcttcttc gatgtctgca
     4921 ccgaggtgct cgcctttccg gtcatccgtg acaagctcgt gctcaacatc accgacggcc
     4981 tgcgcggcca gtacgacggc ggcccggacg gcgccgccca gttcacctgg gattataaca
     5041 gcctcttttt cgccaccgat cccttcgccc tcgacatggt ctgccacaac ctcctgctcg
     5101 ccaagcgcaa ggagatgcag gtcaaggtca acgagcatcc ccgctatacc gaatacctcc
     5161 gctatgccga gaaactcgga ctggggatcg ctgcgccgga gaaaatcagc catgtcgtgg
     5221 tctgagcccc acctcctcgc ggcggcggcg cctctggcca tcatgactct ccgcgctgcc
     5281 gccctccggc tcctgcgcac agttcggagc ggcatggcgg gggtgttgat cctcatgtcc
     5341 ttcgccctca gctccgcagc cgccgccgat ctgcccggga ccggattcta tcccggctgg
     5401 tctcccgcag gcaagccccg catctacgaa gcgaaaaacc tctacggcta tattgacggc
     5461 ggggccgaac tctttctcga gttcggtttc agcgggctga cggtccagcg ctacagccgc
     5521 ggggaagagg agctgagcct tgatctctac gagatgactt cggccgacgc cgccctcgcc
     5581 atctacctgg ccaaaaaggg ggaggaaaaa cccgtcgccg gcctctcctg ccgcagcacc
     5641 ggcagcgcct ggcagatcgc cgccctcagg ggacgccatt tcatccagct caccaacctg
     5701 agcggggagg agagcctgct ccccgccatg gtcgccctgc tgcaggcgct gctgcccgcg
     5761 ctccccgatg aaccggcccg cgactggctg acggcgatgc ctgcggagcg catccccggc
     5821 tccgagctgc tgctggctgg cccctggagc ctgcagatga tctacaccct cggcgatgga
     5881 gatctgctcc agctcggcgg cagggtggcg ggagtcgccg tcgaacagcc cggtaaagat
     5941 ggaaagcgag aggcttgcct gcgccttttc tatccggaca gttccgctgc ccgggcggca
     6001 tttgcccata ccaccgccca cctcgatccc tatctcactc cgctgcatgc ggccggcgat
     6061 accctgctct tcagggatta caaagaggaa tacggccgca tcgttcgctg cgacagcgtc
     6121 atccagatcg aactccatct ctcccaaccc tgaccccaca aggaggcgtc tccatgcgca
     6181 gaaaaaccat cctcctcgcc ctgctgatgc ttctctcctc ctccgccctg ctccgggcgg
     6241 gcacggagat catcgcccac cgcggcgcct ccggcctcgc tccggagaac accctggctt
     6301 cgttcgccag ggccgtcgag atcggcgccg actggttcga actggacgtc tggctctcca
     6361 aggacgattc cctcatgctg atgcatgacg agtcggtcgc gcgcaccacc ggcgccggcg
     6421 ggacgatccc gaccatgacc tatgatcagc tgcgcagcct cgatgccggc tcctggttca
     6481 gccccctctt tgcgggagaa aagattccca ccctggccga ggccctggac ctggccctcc
     6541 gcgcctccta tcgcgtcggt gtggtcatcg agatcaagag caacgccgcc accgtggtcg
     6601 acaaagtcat cgccgccgtc cagcagcgcg gcatgcaggg gagggtgatc atcagcagct
     6661 tcaactccag ccatatcagc aagtccaagc agctcgatcc ctccatccag gtccagcttt
     6721 tcggcagcat caccctggca caaatcaggc aggttgacgg catcggcggt gagtgggtcg
     6781 gcaccaacgg cgccatcacc gcggccctgc ttgactcggc tcatgcccgc ggcatgaaga
     6841 tgaacaagtg gaccatcaac agtgcctccg agatgctcac cctgatcaaa cagggggtgg
     6901 acgccatcac caccaatttt cccgacatcg ccagggccct ggtcgacacc accccgccag
     6961 gcgaggtggt gctccttccc cccgtggtcg acgagacccg cattggcctg agctggctcc
     7021 ccgctgagga cccggagagc acctcgatca cctacgaaat ctaccgcggc acccaggccc
     7081 atcccgaaat tctgctggcg accgtgaccg acaccaccgc ctatatcgat gagacccggc
     7141 gcgaatctca gcagtacttt tatcgcatcc gggcgaaaaa tgtcgccggc ctctacagcc
     7201 ccgcctggag caacgaggtg caggcgacca ccgccccgga cctgcgcccg ccgcaggtta
     7261 ccgcagtccg cgcctacggc cggcctgacc acctggtgat cagcttcaac gagcgggtgg
     7321 atggggaaag cgcccgcgct gccgccaact atcagatcgg acccggggtc accgtccaca
     7381 gcgcccgcct cgccctcgac tccctctcgg tcatgctcga caccaccccc ctggccgaca
     7441 agagcagcta caccctgacc atcaccggag tcaaggacca ggccatccat cccaacccca
     7501 tcattgaacc cctcccggtt cccttcacct acatgcccta caggcccggg ctggtcgccg
     7561 cctgggattt cgatcaggag gatggcaata atttgcccga ccactccggc aacgccaatc
     7621 acggcgccct ccagaacggc atcgcctggt caggcggcca caccggcaat ggcctcctct
     7681 tcgacggggt cgacgattac atcctcgtgc ccgcttcctc ctccctcgat atcaacaccc
     7741 aggggattac cctctcgctc tggacctgga tggcctatgc ccccaacgat ctccccggag
     7801 cctatggccc cctctacgac tcagagagcg acacctacgt cctttacgag gacaaaggca
     7861 tccacgagct ccgcttcaag gtgaccacca ccgtcagcgc ggagcggcca ggaattcgcg
     7921 gttcagagct ctccgccggg gaatgggtcc atctggtcgg gctctacgac ggcgccaatg
     7981 cgatgatcta cctcaacggc gaactcaagg acacccaccc cctgaccggc accgtcaagg
     8041 ccggccagat cgccactatc ggcaaaaccg gtggcgccta cttcaagggc aggatcgacg
     8101 acatccagat ctataaccgc gccctcagcg ccgaggagat ccttttcctc tacgacggcc
     8161 gcgagatcac cagctcggcc agccgagacc cccaccagcc ggaggaattc ggactgtcgc
     8221 agaactatcc caatcccttc aatccctcca ccaccctgaa ctaccgtctg agccgccggg
     8281 aactggtcga tctggccgtc tatgacctgc tcggccgcag gatcgccgat ctggtgcacg
     8341 agcttcaggg accgggccag tatcaggcca cctgggacgg gctggacaag aacggcctcc
     8401 ccgccggcag cggcctctac ttctgcaaac tgaagagcgg cggcagaacg attacgcgcg
     8461 ccatgactct gattaaataa aaatccccac cccctttcct agacagggcg ttcctcctgc
     8521 cggcggcagc cattccggga ctgagccagg ccggaggaac gccccaccga ttcactctgc
     8581 agccatcatt cataaaaaca acgccacgcc cgcttgaatt ttttcccctg acgatgagaa
     8641 ttttcttgca atttctgtga ataattagta caattcgcaa gcaataataa ctattaataa
     8701 tcattcgaaa caccatatct ggctcaattc aaccaatcga aagaaaatcc gaccatggct
     8761 cgagggacca gcggcaccgt tctgctcgca gaggagaggc gcaaccgcat tgaggagatc
     8821 ctcaaggtgc aaaatcgcat cttcgtggcg gagatggcca ggcagatgaa gacctctgag
     8881 gtcaccatcc gcaaggatct ggatgttctc gaacggcggg gcgttctgaa gcgggtccac
     8941 cgcggagcga ttctcgtcag caatccaacc gtcatcgatc tcgacctcgt cgagaaagag
     9001 cacatccact cgcgtgaaaa ggataaaatt gcccgcaagg ccgccgacct tatccgtgac
     9061 ggtgaagcga tcatcctcga ttccggctcg acgaccaccg cgatcgcgcg ggcgctcaag
     9121 gcgcgcagcg gactgacgat catcaccaat gcaatcaaca tcgcctggga attgaccgcg
     9181 agcaagaatc aggtcatact aatcggcggg atggtgcgcg aaaactcgtt ctcgctggtg
     9241 gggccgctct ccgaggaggc tatgcgcaac ctcagcgccg acaagctttt tctcggcgtc
     9301 gatggcatcc atttcgagtt cggtctgacc acccccaacc ttcaggaagc gaccatcaac
     9361 cgcatgatgc tgcacggcgc caccgatgtc ttcgtcgtcg ccgactcgag caagatcggc
     9421 cgccgcagcc tgggggttat cgccgaggtc accgccgccc accatctcat cacggacgat
     9481 cacatcacac cggccgaccg ggaaaaactc cgggagctcg ggattgacgt gatcatcgcc
     9541 tgaccttcat atcaaaaaga cgggatccac gcatgcaact aaagtcacaa ggccccaaga
     9601 ggcatatgca aaccggtctc actctgctct tccttctctc cagcttcacc gctgccctgg
     9661 cttcctcccg gctgcccatc gacagcagct ccgattacct ctgctactac ggctcatggg
     9721 acaacgacaa gctcttccgc gccaaagact ttgatctggt catcctcgaa ccctcgaaca
     9781 tctccgccgc ccagatcgcc gagatgaaaa agggccatga cggcctcgcc ggcacccccg
     9841 atgatgtcat cgtcatcggc tacgtctcga tcggcgagga ccatcagggg ggacggaccg
     9901 gcgatggccg tggcccctgc tactgggatt ggggttccag ctccgtagtc tataccaatg
     9961 gcgggtatgc ctcctggtat ctcgatgacg ccaaccacga caagaaaccg gatatgaacg
    10021 gcagctgggg aagctattac gtcaacgccg gcgactcctc ctggcgggcc tttctcaaga
    10081 gcagcccgac cggcaccgat aatattctca ggaccaagaa ctgcgacgga ctctttctcg
    10141 acaccatcga tactgcctcc ccctggtccc cctggcccta ccgctggatg gtggtggcga
    10201 tgtccgatat ggtcggctgg ctgcacacga tctacccgga caaatacctg gtccaaccgc
    10261 gggctcttct atttcgaccc cgatctcgcc actgcctacg cccactcgat ccgccccttt
    10321 atcgacgggg tgatgttcga atcctattac aaggagggtg agcgggcggt ctgggcggcc
    10381 aaggtcaatg cggaggccgc gaaacccgac ggatttgccg tcatcgccct cgattactat
    10441 tcagcaagcc aaaccgccgc catcgacaag caggtcgcag aggttctggg gctgggctgg
    10501 agcgattaca tctccagcac ttccctcaat gaaatccgct atgaggcctt tcaccgccac
    10561 cccgtcgatg tcaatccccc gacctggaaa tcatcgatcg gcctcatcga ggcggccact
    10621 tcggatggtg cagtcaaatt gaggtggagg caggtcaccg accagtcggc cccggtctcc
    10681 ttcacggtct attacagctc tgcacctctc ttcagcatcg aaggggcgac ccgcctcgaa
    10741 caggtgcccg cgacctggca gagcagcagc ctctcctggg agtatcagct caccggactg
    10801 gccaacttta ccacctacac ctttctcgtc cgcgcccggg acgcccttgg caatgaagac
    10861 aaaaatctgc gcctcctcaa ggccacaccc ccagccggca tgaataccct gctcaccatc
    10921 gatggtctct tcaacgactg gggcgggatc gcaccactcg acaccccgcc caatcccgtc
    10981 gaagcaggtg gttacggtct cgatgccgcc gccgacatcg tcgacatcta tgccgctcac
    11041 gacgccgcca atttctatct ccagttcacc accgccgccg cctttggcag cggctatttc
    11101 taccatgtct tcttcgacat cgacaacaac ccggccacgg gctacctcta tggcgacagc
    11161 gcatcaaccg ggtctgaact gatgatggag aatctcgccc tctggcagta caccggcacc
    11221 ggcggcagca actggagctg gagccaggcc gccggactga cgcgcgccac ctctggcagc
    11281 cagaccgaac tgagcatccc gctcagcctg atcccggcgg ccgtgcagca gggcaagatc
    11341 accctgctgc tgcagaccaa tcgtgccgtc tcacccttca cctttgtcga cgccgcccct
    11401 gaccgcttcg gcagtgagcg tctcgtctac tctttcaaca gcggcaccgc tgtgcccgtc
    11461 gaacctgcgg ccgcccggcc cgggggactc atcctcggcc agaactaccc caaccccttc
    11521 aacagcacca cgcgcatacc ctttgcactc gacactcccg gcgaggtctc catccgggtt
    11581 ttcaacctgc gcggggagag cgccgcccgc tacgatcttg gcatgaagag cgccggatct
    11641 caccagttca ccctctcggc acaagagtta accagcggca tctattacta cgaactttta
    11701 acaggaggaa aacggcaaac aggcaaaatg atactgatcc ggtaggcggt gctcatatgt
    11761 gtgcagcctg cccgggaagt gaaggatgtg attacccatt atgaactgtt gcacctctaa
    11821 cgttcaactg cgagggaaaa gatgaaaaaa gaactgctca tttgtttggc cctggtcgga
    11881 atgctgcttt cagggcagcc cgccgtggcg cagatcagca tcgatggtct cttctttgac
    11941 tgggaggccg ccacccaggt ggatgtcgcc ccccaggccg aggagttgac ctttgatctc
    12001 ggcgatccgg attgccccaa tcccgccgtc ccgagttatt tcgccgatct ggacattgag
    12061 gatctcttcg tcaccgacga cgccgacttt gtctatttcc gcatcaagat gaatccggtc
    12121 gccaatctca ccaatgtccc caatgacacc tcctaccatg gcggtgcggc actggctgct
    12181 tacatctcgg ttgatccggg ctgggcggat atcaccggcc tgacctgggg ctggtggggc
    12241 agcggttatg actatttcgt ccaggtctat cccgcggaca gtgccgcctt tgccgccacc
    12301 ggctatgaac agtatgtctg ggagcataaa caggccggca acggctggga tttcattccc
    12361 gccgactcga ctatcggctc cagggtcgcc tggaatgcca ccagcaacga ggttgaattc
    12421 tccgtgccca aatcgctgct cctcaatccc aggtacattc ccgattttgt ctctcccgac
    12481 accatcgcgg tgatgatcta cgcgggtgaa aacctcgcac cgtggcgggc ggattacgcc
    12541 agcaatcccg ggattgcggg tttcaagttc gccctcaaac aacccggccc catcgccatc
    12601 gacggcctct ttttcgactg gactgcggag atgcagcttg atacgccggc caccagtgag
    12661 gagttgacct tcgatgccgg cgatccggac tgccccaatc ccgccctccc cagctatttt
    12721 gccgatctcg acatcgatga tgtctatgcc acggacgatg ccgatttcat ttacctccgc
    12781 atcaagatga attcgatcgc caacctgacc aatgtgccca atgacacctc ctatcacggc
    12841 ggcgccgccg ttgccgccta catctcggtc gatccgggcg cggctgacac caccggcgta
    12901 acctggggct ggtggggcaa cggctacgac ttcttcgtcc aggcctatcc ccccgattcc
    12961 gcctatgcgg ctgtcggcgg ctatccccag ggggtctggg agcacaaaca ggcgggcaac
    13021 ggttgggatt tcgaggtggt cgactctacg cgcggcgcct gggtggcctg gaatgccctc
    13081 aacaatgatg tcgagatggc catccccaag gcccagctcc tcaatccccg ttatctgcag
    13141 aactttcagc agccggacca gatcgccatc atgatctacg ccggcgagaa cctcgctccg
    13201 tggcgcgcgg attacgcctc gatgcccggc gtggcgggtt ttctcctcaa cctcggcggg
    13261 tcgaccggtg tcgccacccg gccggcgggc cggattcccg tcaacatggc cctggaacag
    13321 aactatccca atcccttcaa tcccgagacc cgcatccgct ttgccctgcc cgcgcagggg
    13381 agtgtgcgga tccgcgtctt cgatctgctc ggcaaggagg tcgcctccct ggctgacggc
    13441 gttttcagcg ccggcgagca tgtcgtcacc ttcagcggca aggatctgcc cagcggcgtc
    13501 tatttttatg ccctcgaggc tggcgggtcg cgtgaaatcc gcaagatgat gctgatcaaa
    13561 taactgtctt tttcaagcga gctggcaggg tgactccatc tctgccagcc gctcgtatcc
    13621 atgcgtccag gggcggagcg tttggatctg gcataacaga cttacatgag gagaatcgaa
    13681 gatgagaatt accctccgct tttttctctt tttcctgctg agcttctccc tggccgcatt
    13741 tgcccagact gtccattacg ttgaagcggg cccgggcgcc atctctgcgg cctaccaggc
    13801 cgctgccgcc ggagatatca ttgagctggt cacttccggc ggaacctata ccgagagcgc
    13861 gaccatggaa atcaccgccg acaaggcaat caccatccgt gctgcggccg ggatcacctc
    13921 gccggtttgg cagtgcgagg cctcaccctt catgaccgtt ggcggtacgc tgatcatgga
    13981 gggcctcact ctggacgggc aggaagccgc aaaaagggcc atcaacacca ccggcagcgc
    14041 cggctattcc atcaaaatcg tcaacagtac cttgcaggga ttcgaataca tgatctacgg
    14101 aagctcctcc tccgctctgg attccctggt tattctcaac tcgctcttta aaaaagctac
    14161 caaccgggcc ctctattttc cagccggcac catcgatccc ggtgtggtca agcgcctctc
    14221 catccgcaac tccaccttca tggagatgaa aaaaaccgag ccttccatct atttttacgc
    14281 caagagctcg ccgacggtca gtccccgtct ggaactggac catgtcacct tttacaactg
    14341 ccagcgcatc cgcaccaaca gcggcgtcac cgatgtcagt atccgcaata ccatctttgc
    14401 caccgatggc acgatcagcg gcggacagtc cttcaacctc aacggcggcg tcgtcgagaa
    14461 taccctggtc ttcaatgccc cggtctccgg atccggtgcc agctattccg gctttatgga
    14521 caatgtggat cccctcttca ccgacgccgc caacggcgat ctcaccctcc tggaggccac
    14581 accggccttt catgccggca gtgacggcaa aaccctcggc gatccgcgct ggtggcccag
    14641 actgtccggc cgcatctatg tcgcagcggg cagggatcag atcgccgctg ccgtggccgc
    14701 ggccaatccg ggcgacgtca tcgaactgac cagtgacggc gggctctacg tcgagtcctc
    14761 cacagtagtc atcgacaagc cggtcacgat tcaaccctcc gagagcatcg tcaatccacc
    14821 ggtctggacc tccgatgacg gcggctacct gatcctcacc cgggccggac tgaccctgac
    14881 cggcgtcgtt ctcgacggcg gcaagggcgc gctccaggcg gccggcggca tcgccaccga
    14941 ttcgagcggc tacaatctga tcctcacggg gtgcgatttt ctcaactttg gcggcgagag
    15001 cagcacgacc ggtcatgcca tctatgacgg cctctatcag ggcgaggtgg attcgctggt
    15061 catctcgagc tgctatttta actccatcgc ccgcgagggc atctacctgg gcggacgcac
    15121 tgcggcccaa atcggcaccg tcaagtattt ccacatggtc aactcgacca tggccaggat
    15181 cggcgatgac gcggtctata tccgtgacca cgatggcaat ttctccaccc ccggtcccgt
    15241 ctacctcatc gatcactgca ccttttatga cgccttcgat tcctatggcc tcctggccca
    15301 ttacatcgac aatgcactga tcaaaaacac catcgccgcc gcctcgacgg taaaaggcac
    15361 ggctttttac ctctatggcg acaattcgct ggttaaaaac tccctctatt tta
//
LOCUS       DAOPWU010000038        15385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3913_length_15385_cov_5.745205, whole genome shotgun sequence.
ACCESSION   DAOPWU010000038 DAOPWU010000000
VERSION     DAOPWU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15385
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3913_length_15385_cov_5.745205"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKM23_03685"
     CDS             <1..213
                     /locus_tag="PKM23_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90608.1"
                     /translation="ELMQAGVLHGGEGKPIYFNGGGWMLLLFFLVMAAPFIADKSPNP
                     MAGLIAVIVLKTLGELFGVWAARIAK"
     gene            290..1177
                     /locus_tag="PKM23_03690"
     CDS             290..1177
                     /locus_tag="PKM23_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNY90609.1"
                     /translation="MLPFDTSVIELIKQRHSCRTYEKRAIAGETAARLAAFCASVTSG
                     PFGTPLRFELAAAAGDDPGALKGLGTYGFIKEPAGFIISAVAPGEKNLEDFGWGMEAI
                     LLYATGLGLGSCWLGGSFAKSRFANRIHAGAAEIVPAVAAVGYRAEETPLRRMMRSQI
                     KSDSRKLWEALFFRDDFGTSMRAAEAGAYQVPLAMLRLAPSASNSQPWRVVQKGECYH
                     FYLQRKPGFTPGRASLIHFTIADLPRIDLGIAMCHFQWTAEELGMKGRWELHEEGIPG
                     ADETREYIASWNGSNIMAR"
     gene            complement(1259..2158)
                     /locus_tag="PKM23_03695"
     CDS             complement(1259..2158)
                     /locus_tag="PKM23_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNY90610.1"
                     /translation="MKSQSLTRYAWLSIAAAVLTIGLKAAAYLLTDSVGMLSDAMESM
                     VNLAAAVMALAMLIVAARPPDELHAFGHSKAEYFSSGLEGALILLAAVMIGLTAIPRL
                     LAPQPLEKVGLGLLVSSAASLVNLAAGRVLLQAGRKHQSITLEADAHHLMTDVWTSAG
                     VLFGIAAVALTGWERLDPIIALIVAANIIWTGFQLLRRSALGLMDTAIPDSELAQVRQ
                     VLDSYSGRDVHYHALRTRQAGTRKFISVHILVPGEWPVQQGHNLVGEMEEKIRSLLPE
                     AHLFTHLEPINDPAASQDINLDA"
     gene            2615..2962
                     /locus_tag="PKM23_03700"
     CDS             2615..2962
                     /locus_tag="PKM23_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNY90611.1"
                     /translation="MTEPVDILKALSDLGRLRILKMLEVKSLCVCEITEVIGLATSTI
                     SAHLSQLRNAGLIEDEKEGKWINYRLADDPDPLFTELWPLLSRRLDEEDAIRSDREQA
                     CHIDRHRICSAGK"
     gene            3137..4432
                     /locus_tag="PKM23_03705"
     CDS             3137..4432
                     /locus_tag="PKM23_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNY90612.1"
                     /translation="MDWKKEIKTLLWIVVTFVFLYYMPIGSKRFDNAIMEAFHLVKWY
                     AREHVLLCLVPAFFIAGAIGVFVSQESVMKYLGPKSDKWLAYGVASISGTILAVCSCT
                     VLPLFSGIYTMGAGLGPATAFLYSGPAINVLAIILTARILGLELGIARAVGAIVFAVI
                     IGLLMHFIFRHEKMEEEGPVLVEGSKAGRPLWQTAIYFALMIAILVLANWGKPAVESG
                     FWHSVYTLKWTLTAASALGLAVVLGLWFKISWLRLALAAAPAILLGLFTPASPVLIFA
                     AGSIGLAVITAMDKGEAGEWFAASWGFAKQILPLLLIGVFIAGALLGRVGQEGLIPSA
                     WVSQAVGGNSLFANFFASIAGAFMYFATLTEVPILQGLIGSGMGKGPALALLLAGPAL
                     SLPSMLVLRSVMGGKKTLAFVTLVVIMATITGLLFGWLF"
     gene            4510..4767
                     /locus_tag="PKM23_03710"
     CDS             4510..4767
                     /locus_tag="PKM23_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNY90613.1"
                     /translation="MKKLQILGMGCAKCNKLMEMTEQAAKELGIEYEIVKVNTLDEIK
                     KFRIMMTPALAVDGVVKVYGRVPGLDELKQMIKRGSNERSI"
     gene            4751..5542
                     /locus_tag="PKM23_03715"
     CDS             4751..5542
                     /locus_tag="PKM23_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNY90614.1"
                     /translation="MSEAYEEKIHAAVRARYRAAVQQGGSCCGGDSGCCGGGDPGRES
                     ARLGYSAAELGTVPAGANLGLGCGNPQALAALKPGEVVLDLGSGAGFDCFLAAQQVGE
                     NGYVIGVDMTPEMVARARANAREGGYNQVSFRLGEIEALPVADNLIDVIISNCVINLS
                     TDKLAVFMEAFRVLKPGGRLAISDVVATAELPLEIRANLDLYAACAAGALTIAELKVL
                     LAEAGFARIEIKPKDESRSFITDWVPGRNLEEYIVSAAITAVKPA"
     gene            5634..6056
                     /locus_tag="PKM23_03720"
     CDS             5634..6056
                     /locus_tag="PKM23_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNY90615.1"
                     /translation="MDRYLTLWIFLMVGFLLINLSACKSARNEPDSVKAAGHQAKITF
                     VELGSINCIPCKAMQPVMKAIEEKYGDQIKVVFYDVWQPEQKKYAQQYRIRLIPTQVF
                     LDASDKEIFRHEGFFPEEEIDTLLQSHGLKPAKKESKT"
     gene            6089..6760
                     /locus_tag="PKM23_03725"
     CDS             6089..6760
                     /locus_tag="PKM23_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNY90616.1"
                     /translation="MAGGFHLALLASLLWGILSILLSPCHLSSIPLIIGYITSRESAT
                     TRQSFLLALVFALGILVTIALVGAVTASLGRLIGDVGVWGNILVAAIFIVMGLYLLDV
                     ISLNWSSVPLTAGRTGYWGAFGLGLLFGIGLGPCTFAYMAPVLGIVFTLAQSGWIKPF
                     LLILAFGIGHSAVIALAGGLGRWVQEYLNWSSHSRAAAWVKKGAGVLVLLGGLYFIYS
                     SFLQG"
     gene            complement(6909..7610)
                     /locus_tag="PKM23_03730"
     CDS             complement(6909..7610)
                     /locus_tag="PKM23_03730"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNY90617.1"
                     /translation="MEKIDLQTFHHIVFFTGAGMSAESGVATFRGAGGRWKEYNWQEV
                     ACQRAFDRDPERVLDFHRLRRREAIGCEPHAGHKVIAGLEAAHPGVTVVTQNIDGMHQ
                     RAGTKKIFELHGSLWRVRCPQHGVWEDRDPEFATRRCPHCGSWLRPDIIWFEDMLDDK
                     VVAGATRAILACDLLISIGTSAVVWPAAGYPELARQNGAFCVEINPEPTEISGLYQQV
                     IRGKAGEVLPELFEK"
     gene            7887..9338
                     /locus_tag="PKM23_03735"
     CDS             7887..9338
                     /locus_tag="PKM23_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNY90618.1"
                     /translation="MKHYIAAAVLLLSAGCGSHLPRVQGVAGTSPAPAVPWTPPAREE
                     KPLKLAEATVPEAKLRDRWQQLSLAEAVDIALQNNPDTRAAWANARAAAAAYGAARSA
                     WLPAISADGALARTRQESDPPQPNDYGPSTAYSYGAGVNWLLFNFGGRAAAIDESRQA
                     LLAADWSHNAVIQNTLLQTVTAYYTCAGARAMLEAHRASLAAAETALQAAEEKRGVGL
                     ATSADVLQARTARSQALLDVQEAEGDLRLSLGNLAVAMGYPAHLFGEFTAVIPEIPAD
                     SLTQQVNALVEKALAGRPDLQASRARARAAEANVRQARAGLLPSISASAGADRSRLYG
                     GERQSDTYSGALRLEMPLFAGFSRHYQLARARAEADAAKEAVRAAEQEVSYQVYASHS
                     DFLTARARVATTDDLLASAQQSEQVTLGRYREGIGSILDLLTAQRSLAQARAQQVNAR
                     LSWHIALARLARDIGQLGAHGENPLVPASTQTR"
     gene            9342..10571
                     /locus_tag="PKM23_03740"
     CDS             9342..10571
                     /locus_tag="PKM23_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNY90619.1"
                     /translation="MKPRTAIYIMLAMSVLAGCSKEKARQIPPAPVTTAQVALQAQPL
                     ALNVVGLVEPIESVAVKPQAGGVITGVHFMEGGDVRAGQLLFRIDPRPYQAALHAAEA
                     QLARDSSQAVYTAMQAQRYAGLSEKDFVTREQYEQARTQAEMFKAALKVDQAAVEQAR
                     LNLGYTAVTAPISGRAGALLVKRGNVVSPNGSALVVINQMDPIRVGFAIPGTELPLVQ
                     KWAAGGTLTVHVRPSRNGAEEEQMTGRLSFLDNAVDAATGTVALKAEFANEREQLWPG
                     QFVDVELVLTIENEALTVPVSALVTGQEGTFVYVVTADNKAEKRAVQVNRTLGGIIVL
                     DGGVQAGEQVVTDGQVKLVPGARVELRKGAAAGGSRTGNAGGTSGTPSGSGAGTPAGS
                     GTAEGASKIGQSTGRPE"
     gene            10568..13642
                     /locus_tag="PKM23_03745"
     CDS             10568..13642
                     /locus_tag="PKM23_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNY90620.1"
                     /translation="MRIFIDRPVMTTLVMIAILVAGFIGYRQLPVSDLPTVDFPTISV
                     SASLPGASPETMAAAVATPLEKQFTTIAGIDNMTSSSSLGSTNITIQFALDRDIDAAA
                     ADVQSAIAAVSRQLPRDMPTPPSYRKVNPADSPIMFIAMTSPTLPLYTIDELADIMIA
                     QRISMVSGIAQVQVFGGQKYAVRVQVDPRELAARSIGIDEVASAIVANNVNLPTGILW
                     GSEKALTVKATGQLNSAAEYRPVVVAYRNGAPVRLQELARIYDSVQNNRVAAWMNDDR
                     CILLAIQRQPGTNTVEVVQNVRRLLPQIQEQLPASAQVHILFDKSEPIQESVNDVQMT
                     LLLTLFLVILVIFLFLRNVSATVIPSLALPLSVVGTFAVMRLMDFNLDNLSLMALTLA
                     VGFVVDDAIVMLENIFRHMEMGKSPYQAAVDGAREVSFTIVSMTLSLAAVFIPVLFMG
                     GIMGRLFQEFAVTIMVAILISGFVSLTLTPMMSSRFLKHEKKESHGRFYNAIENFWNK
                     SLGFYERTLTWVVDRRRATMLFSLVILLLTVFLFIVVPKGFLPSEDTGLLNGSFEGAE
                     GTSFEGMLDDREQVTRILAADPNIERFTVNIGGMGGTANTGRMNIRLKHRSERELNAD
                     EVANSLRRKMASIPGARVFLVNPPVINIGGRFAAALYQFTMQSTDIQALYAAAPLLER
                     RLRADPMFKDVNSDLRISNPEVIVAIDRDRAASLGLSAQTIEEALYSSYGTRQVSTIL
                     APNNQYYVMLELLPEYQRDASAFQYLYVRSPQTDKLVPLSTLATISPGAGPLTVNHSG
                     QLPSVTVSFNLDDGVALSEGVAAVNRAARETLPASIATSFSGTAQAFQSSQSSLLALL
                     FLAILVIYMILGILYESFIHPLTILSGLPFAVFGALVTLLIFKMDLNLYSFVGLIMLI
                     GVVKKNAIMMIDFAITARDEGLGAREAILRACSIRFRPIMMTTVAALMATLPIALGMG
                     AGAESRRPLGLAVVGGLLFSQMITLYVTPVFYIYLEKLQNRLGRVFNKGVRA"
     gene            complement(13713..14381)
                     /locus_tag="PKM23_03750"
     CDS             complement(13713..14381)
                     /locus_tag="PKM23_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNY90621.1"
                     /translation="MRKLMMVLLVAVLSVGMISCASMTKATQGAAVGTAAGGAAGAVI
                     GNQVGNTALGAIIGAAIGGAAGAYIGNKMDQQAAEIEKELADAEVERVGEGIKVTLKS
                     GLLFDTGKSVLKPAAQADLEQLAVTLNKYSGTVLRIEGHADSQGAEDYNQKLSESRAH
                     AVSSFLAKSGVDISRFTIMGYGESQPIADNATVEGRAQNRRVEIAITAGDGMKAEAQA
                     ATTK"
     gene            complement(14756..>15385)
                     /locus_tag="PKM23_03755"
     CDS             complement(14756..>15385)
                     /locus_tag="PKM23_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY90622.1"
                     /translation="HNNIYEYHFKGLLDEMSFWNMALTPEQIAARMFDRPRGTEEGLV
                     AYYSFDEGGGQVAHDNGPNMLHGRLGKMTETDESDPLWISIDRPARMAALAVLEKSYA
                     PLEEVIEQEEIPLTCELGQNYPNPFNASTTISYNLPEMDEAGSLRLDIFDVQGRLIRT
                     LVNGTAASGSHQVLWNGANEQGEPVPSGIYFYRLQAGSFQEIKRMVLLK"
BASE COUNT         2926 a         4615 c         4711 g         3133 t
ORIGIN      
        1 gagctgatgc aggcgggcgt cttgcacggt ggtgaaggca aaccgatcta ttttaacggc
       61 ggcggctgga tgctgctgct tttttttctg gtcatggccg cccccttcat cgccgacaag
      121 agccccaatc cgatggcggg gctgattgcg gtcatcgtcc tcaagacttt gggcgagctc
      181 ttcggcgtct gggcggcgcg gattgccaag taaaagtaat gatgccagcg catagctgca
      241 tcaccaagta aaaaagcgat ttcagcacat ctctggcgaa ggaagactca tgctcccatt
      301 cgacacctct gtaattgagt tgatcaaaca gcggcactcc tgccgcacct atgaaaaaag
      361 agcgatcgcc ggggagacgg ccgcccggct ggcggccttt tgcgcttcgg tcaccagcgg
      421 gcctttcggc acgcccctgc gctttgaatt ggccgctgcg gccggggatg atccgggtgc
      481 gctgaagggc ctgggtacct atgggttcat caaggagccg gccggattca tcatcagcgc
      541 ggtagcgcca ggtgaaaaga acctcgagga tttcggctgg ggcatggagg ccattctgct
      601 ctatgcaacc ggtctcggac tgggaagctg ctggctcggc ggcagttttg ccaaaagccg
      661 ttttgccaac cgcatccatg cgggtgcggc ggagatcgtc ccggctgtag ctgctgtcgg
      721 ttatagggcc gaggagaccc cgctgcgccg gatgatgcga agccagatca aatccgattc
      781 gcgcaagctg tgggaggcgc tctttttccg cgacgatttc ggcacatcga tgcgcgcggc
      841 ggaggctggc gcttatcagg ttcctttggc catgctgcgc ctggcgccct cggccagcaa
      901 cagccagccc tggcgggttg tgcagaaggg cgaatgctat catttttatt tgcagcgcaa
      961 acccggcttc acgcccggcc gtgcttcact gatccacttc acgatcgccg atcttccgcg
     1021 catcgacctc ggcatcgcca tgtgccactt tcaatggaca gccgaagaac tgggaatgaa
     1081 ggggagatgg gagctgcatg aggagggtat tccgggagca gatgaaacgc gggagtatat
     1141 cgcgagctgg aacgggtcaa atattatggc gagatagtac ttgattttac ctcccggctg
     1201 gctgctttgc cagcaggcag ggagcggaac gcatgtggct gatctggatt atcgtctttt
     1261 aagcatcaag gttgatatcc tgactggcgg ctggatcgtt gatgggctcc agatgggtaa
     1321 acagatgggc ctccggcaaa aggctgcgga ttttttcttc catctcaccg accaggttgt
     1381 gcccctgctg caccggccat tcaccaggta ccaggatatg cacagaaata aacttgcggg
     1441 tgcccgcctg tcgcgtccgg agcgcgtgat aatgcacatc gcgaccggaa tagctgtcca
     1501 gaacctggcg gacctgggcc agttcactat ccgggatggc ggtgtccatc aggcccagcg
     1561 ctgagcggcg cagcagctgg aatccggtcc agatgatgtt ggctgcaacg atcagcgcga
     1621 tgatgggatc cagacgttcc caacctgtga gggcgacagc ggcgatgccg aagagcacgc
     1681 ccgcggaggt ccagacatcg gtcatcagat ggtgcgcatc ggcttccagg gtaatggatt
     1741 gatgttttcg tccggcctgc aaaagaacac ggccggccgc aagattgacc agggaggccg
     1801 ctgaggagac caacaagccc aggccgacct tctccaatgg ctgcggcgca agcagacggg
     1861 gaatggcggt cagaccgatc atcacggcgg ccagcaggat cagggcgcct tccagaccac
     1921 tggaaaaata ctccgccttg gagtggccga aagcgtgcag ctcatccgga ggccgtgccg
     1981 caacgatcaa catcgccagg gccataaccg ctgcggccag attgaccatg ctttccatgg
     2041 catcagagag cattcccaca gaatcggtga gcagataggc cgctgctttc aagccgatgg
     2101 tcaaaaccgc agcagcaatt gacagccacg catagcgggt gagagattgt gatttcatgg
     2161 gtgtggtttt gttcctcaaa ggactcgaat tacgttacac cgcaaacaaa cgccaaacaa
     2221 gtaaagtctt gagttccatc ctgccatcac agatcaccgt cctgcagtac cccccgtccc
     2281 acagacaaac tgcagcccca ttgctaaggt gccccgccgc cggctgctca agcccggctt
     2341 cggcggattg ccgaggccgg cgcagagaat acccaaaaac aagacgacgg aatcgttcat
     2401 ggcattccgt ctggttttag ggtatgattg tggattattc ataaacgtaa tcaatctgcg
     2461 cctgaaaaca aaggttttct tgctgctgcc gcacaggctt tcctgcagtg gtccgtagtt
     2521 cctttctgcc gccaggaatt atcctaaaaa agtcttgcat ccatccctgt atagtattat
     2581 atttctaaaa atatagaaat gaatggagac ctgtatgacc gaaccggtcg atatcctcaa
     2641 ggcgctctcc gacctcgggc ggctgcgcat cctcaaaatg ctcgaggtca agagcctgtg
     2701 cgtctgcgag atcaccgaag tgatcggact ggccacttcc actatttccg cccatttgag
     2761 ccagctcagg aacgcgggac tgatcgagga cgaaaaagag ggcaagtgga tcaattaccg
     2821 cctggcggac gatcctgatc ccttgttcac cgaattgtgg ccgcttctct cccggcgtct
     2881 ggatgaggag gacgccatac gctccgaccg cgaacaggct tgccatatcg atcgtcaccg
     2941 gatctgcagc gccggaaaat aagctttggt gcgcaagcaa gctgaggcca ggcacaggcc
     3001 gataccgggg cccagggtgg cagcggcaga taatttttgc ggttgcagca ctccgaagat
     3061 gatgcgaacg ccggcagcaa gtgtgagctg actgtggagt gctaatccac gaaccgtaga
     3121 gaggaaagag taatccatgg actggaaaaa agagatcaag accctgctct ggatcgtcgt
     3181 cacattcgtt ttcttgtatt atatgcccat cggcagcaag cggttcgaca acgcgattat
     3241 ggaggccttt cacctggtca aatggtacgc gcgcgagcat gtgctgctct gtctggtgcc
     3301 ggcttttttc atcgccgggg cgatcggtgt ctttgtcagc caggaatcgg tgatgaaata
     3361 cctcggtcca aaatctgaca aatggctggc ctatggggtc gcttcaattt caggtacgat
     3421 cctggcggtc tgctcctgca ccgtgctgcc gctcttctcc ggcatctaca ccatgggcgc
     3481 agggctcgga ccggcgaccg ccttccttta ctccggaccg gcgatcaatg ttctggccat
     3541 catcctcacg gcccgcatcc tcgggctgga gctgggcata gcgcgagcgg ttggcgccat
     3601 tgtttttgcg gtcatcatcg gcctgctgat gcatttcatc ttccggcacg aaaaaatgga
     3661 ggaggagggt ccggttctgg tcgaggggag taaagccggc cggccgctct ggcagacggc
     3721 gatctatttt gccctgatga tcgcgatcct ggttctcgcc aactggggca aacctgcagt
     3781 ggagagcggc ttctggcatt ccgtctacac cctgaaatgg actctcaccg cggcaagcgc
     3841 cctcggactc gccgtggtac tcggactctg gttcaaaata agctggttgc gccttgctct
     3901 ggccgccgcc ccggcgattc tattgggact cttcacaccc gccagtccgg tactgatctt
     3961 cgcggccggt tcgatcggtt tggcggtcat caccgccatg gacaagggtg aagccggcga
     4021 gtggttcgct gccagctggg gttttgccaa acagatcctg ccgctgctgc tgatcggggt
     4081 ttttattgcc ggtgccctgc ttggccgtgt tggccaggag gggctgatcc cctccgcctg
     4141 ggtgagtcag gcggtgggcg gcaactccct cttcgccaac ttttttgcct cgatcgccgg
     4201 cgccttcatg tatttcgcca ccctgaccga ggtgccgatt ttacagggac tgatcggcag
     4261 cgggatgggc aaaggcccgg cgctggcgct gctgctggcg ggtcctgcgc tttcactgcc
     4321 gagcatgctg gtgctgcgct cggtgatggg cggaaagaaa accctggcgt ttgtcactct
     4381 ggtggtgatc atggccacga tcaccggatt gcttttcggc tggctgtttt aaggaggtca
     4441 gactgccgcc ggtttccccg atctggccgg gcacaacgta tccattgaaa tgagacagga
     4501 gctttcatta tgaaaaaatt gcagattctc ggcatggggt gcgccaagtg caacaagctg
     4561 atggaaatga ccgagcaggc ggccaaagaa ctgggcatcg aatatgagat cgtcaaggtc
     4621 aacaccctcg acgagatcaa aaaattccgc attatgatga cccccgccct ggcggtggat
     4681 ggcgtagtca aggtctacgg ccgggtgccc ggactggatg aactgaaaca gatgatcaaa
     4741 agaggttcaa atgagcgaag catatgaaga gaaaatccac gcagcggtgc gggcccgcta
     4801 tcgagcggct gtacagcagg gcggctcctg ctgtggcggg gattccggct gctgcggagg
     4861 cggcgacccg ggccgggagt cagcgcgcct cggctactcc gccgccgaat tgggaactgt
     4921 acctgccggg gccaacctgg ggcttggctg cgggaatcca caggcccttg ccgcgctcaa
     4981 gccgggcgaa gtggtgctcg atctcggcag cggcgccggg ttcgactgct ttctcgccgc
     5041 acaacaggtc ggcgaaaacg gctatgtcat cggtgtcgac atgactcccg agatggtcgc
     5101 ccgggctcgc gctaacgccc gtgaaggcgg ctacaaccag gtctcattcc gtctgggcga
     5161 gatcgaggca ctgccggttg cagacaatct gatcgatgtc atcatctcca actgtgtgat
     5221 caatctctcc accgacaagc tggcggtctt tatggaggcc tttcgcgtac tcaagccggg
     5281 cggccgcctg gccatctccg acgtagtggc gaccgcggaa ctgccgctgg agatccgtgc
     5341 caatctcgat ctctatgccg cctgcgccgc cggcgccctg accatcgcgg aactaaaagt
     5401 gctgctggcc gaagcgggtt tcgcccggat tgaaatcaag ccgaaggacg agagcagaag
     5461 ctttatcaca gattgggtgc cgggacgaaa tctggaggag tatatcgttt cggccgccat
     5521 caccgctgtc aaacccgcat gatggaacga ttctgccggc aacctgtcgg ggctaagagt
     5581 tgtcgccgtt aaaactggag aataagatgg gtacagccgg aaaattatcc ttaatggacc
     5641 gctacttgac gttgtggata tttctcatgg tcggttttct gttgatcaat ctttccgcct
     5701 gcaagagtgc caggaacgaa ccggacagcg tcaaagcagc cgggcatcag gccaaaatca
     5761 cctttgtcga gctgggttcc atcaactgca tcccctgcaa ggccatgcag ccggtgatga
     5821 aggcgatcga ggaaaaatat ggcgatcaga tcaaggtggt cttttatgat gtctggcagc
     5881 cggagcagaa aaaatacgcc cagcaataca ggatccgcct gatccccacc caggtctttc
     5941 ttgatgcctc ggacaaggag atcttccgtc acgagggatt tttccctgaa gaggagattg
     6001 acactctgct gcaaagccat ggtttaaaac ctgctaaaaa ggaaagcaag acgtgatcga
     6061 cagcctcttc agtcatctca acggcctcat ggccggcggt ttccatctgg ccctgctggc
     6121 ttccctgctc tgggggattc tgagcatcct cctcagtccc tgtcatcttt ccagtattcc
     6181 acttattatc ggctatatca ccagccgaga gagcgcaacc acgcgccaat cctttttgct
     6241 ggcgctggtc tttgcgcttg gcattctcgt caccatcgcc ctcgtcggcg cggtaaccgc
     6301 atcgctgggc cggctgatcg gggatgtcgg ggtttggggc aacatcctgg tggctgccat
     6361 ttttattgtt atgggacttt atctgctgga tgtcatttcg ttgaactgga gcagtgtgcc
     6421 attaaccgcc ggccgcacgg gctactgggg cgcttttggc ctgggtctgc tgttcggcat
     6481 cggcctgggg ccttgtacct ttgcttatat ggcaccggtg ctcgggattg tcttcacgct
     6541 ggcgcagtcc ggctggatca agccgttttt gctcattctg gccttcggga tcgggcacag
     6601 cgcggtgatc gctctggcgg gtggattggg ccggtgggtg caggagtacc tgaactggag
     6661 ttcgcactcc cgtgctgccg catgggtgaa aaagggcgcg ggcgtgctgg tgctgctggg
     6721 agggctctat tttatctatt cgtcatttct gcagggttga tggaaagcct ctgaacatcg
     6781 ccccgggact ggctgcgacg ggcatgcact tgaatggaag agtgctgatg ctcgtgacga
     6841 tttccttgag caagcgctgg attaaaatga acggcaaaaa ggaacatcca ggcaattaca
     6901 gataagggct atttttcaaa cagctccggc aggacctcgc cggccttgcc gcggatgacc
     6961 tgctgataga ggccgctgat ctccgtaggc tccggattga tctcgacgca gaacgcgcca
     7021 ttctgccgcg ccagctcggg atagccggcc gcgggccaga ccactgccga ggtgccgatg
     7081 ctgatgagca ggtcacaggc caggatcgcc cgggtcgccc cggccaccac cttgtcatcc
     7141 agcatatcct caaaccagat gatatcgggc cgcagccagc tgccgcaatg agggcagcgg
     7201 cgggtggcaa actcgggatc ccgatcctcc caaaccccat gctgcgggca gcggacccgc
     7261 caaaggctgc cgtgcagctc gaagatcttt ttcgtgccgg cgcgctgatg catgccgtcg
     7321 atattctggg tcacgacagt gacgccggga tgtgcggcct cgaggccggc gatcaccttg
     7381 tgccccgcat gcggctcgca gccgatcgcc tcccggcggc gcaggcggtg aaagtcaagc
     7441 accctctccg gatcgcggtc aaaggcgcgc tgacacgcga cttcttgcca attgtattcc
     7501 ttccagcgtc cgcccgcccc gcgaaaagtg gcgactccgc tctcggccga catgccggcg
     7561 ccggtgaaga agacgatatg gtgaaaagtc tgcagatcga ttttttccat ggttctccct
     7621 tcctggttac atcctgtata atctaacctt tttgggggcg ttcgcaaggg atttgtgcgc
     7681 ggccgggtag gaacgagatt gggggcgcaa gggatttgtg cgcggccggg tgagaacgag
     7741 agccgggggc gcaggcggcg tcggtcatgc catttttcct gttgtttatt tcccgtaata
     7801 ttgccatttt ttgcacaatt gttttatgtt catggagccg ccgggtgatg tgaatcctgc
     7861 cgctctcttt acgatgaggt atacggatga agcattacat tgcagccgcg gttctgctgc
     7921 tctccgccgg ctgcggatcc catctgccgc gtgtacaggg ggtggcaggc acctccccgg
     7981 ctccggcggt gccctggacg ccgccggcgc gcgaggaaaa accactcaag ctggccgaag
     8041 cgactgtgcc ggaggcgaag ctgcgcgatc gctggcagca actgagtcta gccgaggcgg
     8101 tcgacatcgc cctgcagaac aatcctgaca cgcgggcggc ctgggccaat gcccgcgccg
     8161 ccgcagccgc ctacggagcc gcccgcagcg cctggctgcc cgctatcagc gccgacggcg
     8221 ccctggcccg cacccgccag gagagtgatc cgccgcagcc taatgattat ggtcccagca
     8281 cggcctacag ctacggcgcc ggagtgaact ggctgctctt taatttcggc gggcgcgccg
     8341 cggccattga cgagagccgc caggccctgc tcgcagcgga ctggagccac aatgcggtca
     8401 tccagaacac cctgctgcag acggtgacgg cgtactatac ctgcgccggc gcacgggcca
     8461 tgctcgaagc ccaccgcgct tcgctggccg cggctgaaac cgccctccag gcggctgagg
     8521 agaagcgcgg ggttggcctg gcgaccagtg ctgatgtgct gcaggccagg accgcccgct
     8581 cccaggccct gctcgatgtt caggaggccg agggcgactt gcgcctcagc ctcggcaatc
     8641 tggcggtggc gatgggctat cccgcccatc ttttcggcga atttacggcg gtcatccccg
     8701 aaatcccggc cgattccctg acccagcagg tcaatgccct ggtggaaaag gctctcgcgg
     8761 ggaggccgga cctgcaggcc agccgcgccc gcgcccgagc cgccgaggcc aatgtgcgcc
     8821 aggcccgcgc cggactgctg ccttcgatat ccgcctcggc tggggcggac cgcagccggc
     8881 tttatggcgg agagcgccag tccgatacct acagcggagc cctgcgactg gagatgcccc
     8941 tcttcgccgg cttttcccgc cattatcagc tggcgcgggc cagggctgag gccgacgccg
     9001 cgaaagaggc ggtgcgcgcc gccgagcagg aggtgagcta tcaggtctac gccagccaca
     9061 gcgactttct caccgcccgg gcccgggtcg cgacgacgga cgatctgctc gccagtgcgc
     9121 agcagtccga gcaggttacc ctcgggcgct accgtgaagg gatcggcagc attctcgacc
     9181 tgctcacagc ccagcgcagt ctcgcccagg cgcgcgccca gcaagtcaac gcccgcctct
     9241 cctggcatat cgccctggcc cgactggccc gcgatatcgg tcagctaggc gcgcacggag
     9301 aaaaccccct tgtccccgca tccacccaaa cgaggtagtc catgaagccc agaacagcga
     9361 tttacatcat gctggcgatg tcggttctcg ccggatgcag caaggagaag gcccggcaga
     9421 taccgccggc gccggtgacg accgctcaag tagcccttca ggcccagccc ctggccctca
     9481 atgtcgtcgg cctggtcgag ccgatcgaga gcgtggcggt caagccgcaa gcggggggtg
     9541 tgatcaccgg ggtccatttt atggagggcg gggatgtccg cgccggtcag ctccttttcc
     9601 gcatcgatcc gcgtccctat caggcggccc tgcacgctgc cgaggcgcag ctggcgcggg
     9661 acagttcgca ggcggtctat acggcgatgc aggcgcagcg ctacgccggc ctgagcgaaa
     9721 aggatttcgt cacccgcgag cagtatgagc aggcgaggac ccaggcggag atgttcaagg
     9781 ccgccctcaa ggtggaccag gccgcagtcg agcaggcccg cctcaatctc ggctatacgg
     9841 cggtgaccgc gccgatctcc ggccgcgccg gggccctgct ggtcaaacgc ggcaatgtag
     9901 tcagccccaa cggttccgct ctggtggtga tcaaccagat ggatcccatc cgggtcggct
     9961 ttgccatccc cggcaccgaa ctgccgctgg tgcaaaagtg ggcggcgggg ggaaccctga
    10021 cggtgcatgt ccggccctcc cgcaacggcg cagaagagga acagatgacc ggccggctga
    10081 gctttctcga caatgcggtg gatgcggcca ccggcacggt ggcgctcaag gcggaattcg
    10141 ccaacgagag ggagcagctt tggccggggc agtttgtcga cgtcgaactg gtgctcacga
    10201 tcgagaacga ggccctgacc gtcccggtca gcgcactggt gaccggacag gaagggactt
    10261 ttgtctacgt ggtcaccgcc gacaacaagg cggagaaacg ggcggtgcag gtcaaccgca
    10321 cgttgggtgg tatcattgtc ctcgacggcg gggtgcaggc aggcgagcag gtggtgaccg
    10381 acggtcaggt caagctggtg cccggcgcac gggttgagct caggaaagga gcggccgccg
    10441 gcggcagcag aacgggcaat gcaggcggaa cctctggcac gccatcgggc agcggggccg
    10501 gcacaccagc cggaagcggc actgcggagg gcgcatcgaa gatcggccaa tccacgggga
    10561 ggcctgaatg aggatcttca tcgaccggcc ggtcatgacg accctggtca tgatcgccat
    10621 cctggtcgcc ggctttatcg gctaccggca gctgccggtg agcgacttgc ccacggtcga
    10681 tttcccgacc atctcggtga gcgcctcgct gccgggcgcc tctcccgaga ccatggcggc
    10741 ggcagtggcg accccgctgg agaagcagtt caccaccatc gccggcatcg acaacatgac
    10801 cagcagctcg agcctgggca gcaccaacat caccatccag ttcgcccttg accgggacat
    10861 cgacgctgcg gcggccgatg tgcagtcggc gatcgctgcg gtctcgcgcc agctgccgcg
    10921 ggacatgccg acgccccctt cgtaccgcaa ggtcaatccg gccgactcgc cgatcatgtt
    10981 catcgccatg acctcaccga ccctgccgct ctataccatc gatgagctgg cggatatcat
    11041 gatcgcccag cgcatctcga tggtgagcgg cattgcccag gtgcaggtct tcggcggaca
    11101 gaaatatgcg gtgcgggtcc aggtcgatcc gcgcgagctg gcggcgcgca gcatcggcat
    11161 cgacgaggtg gccagcgcca tcgttgccaa caatgtcaat ctgccgacgg gaatcctttg
    11221 ggggagcgag aaggccctca cggtcaaggc gaccgggcag ctcaacagcg ccgctgaata
    11281 ccggcctgtg gtggtggctt atcgcaacgg cgcgccggtg cgtctgcagg agctggcgcg
    11341 catctatgac agcgtgcaga acaaccgcgt cgccgcctgg atgaacgacg accgctgcat
    11401 tctgctggcc atccagcgcc agcccggcac caacaccgtc gaagtggtgc agaatgtgcg
    11461 ccgcctgctg ccgcagatcc aggagcagct gccggcctcc gcccaggttc atatcctctt
    11521 tgacaaatcc gagccgatcc aggaatcagt caacgacgtc cagatgaccc tgctgctgac
    11581 cctcttcctt gtcatcctgg tgatctttct ctttctccgc aacgtctcgg ccacggtcat
    11641 acccagcctc gccctgccgc tgtcagtggt cggcaccttt gcggtgatgc ggctgatgga
    11701 tttcaacctc gacaacctct cgctgatggc cctgaccctg gcggtgggct ttgtcgtcga
    11761 tgacgccatc gtcatgctcg agaacatctt ccgccacatg gagatgggca agagccccta
    11821 ccaggcggcg gtggacggcg ccagggaggt gagctttacc attgtctcga tgaccctctc
    11881 cctggctgcg gtctttatcc ctgtgctctt catgggcgga atcatggggc gcctctttca
    11941 ggagttcgcg gtgacgatca tggtggcgat cctgatctcg ggatttgttt cgctcaccct
    12001 gaccccgatg atgtcgagcc ggttcctcaa gcatgagaag aaggagagcc atggccgctt
    12061 ttacaacgcc atcgaaaatt tctggaacaa gtccctgggc ttttatgagc gcaccctgac
    12121 ctgggtggtc gaccgccgtc gcgccaccat gctcttttcg ctggtcatcc ttctcctcac
    12181 cgttttcctt tttattgttg tgcccaaggg ctttttgccg agcgaggata ccggcctgct
    12241 caatggctcc tttgaggggg ccgaagggac ctcttttgag gggatgctgg acgatcgcga
    12301 gcaggtgacc cgcatcctcg cggccgatcc caacatcgag cgctttacgg tcaacatcgg
    12361 cggcatgggc ggcacagcta acaccgggcg gatgaacatc cgtctcaagc atcgcagcga
    12421 gcgcgagctc aacgctgacg aggtcgccaa cagcttgcgc cgcaagatgg cctcgatccc
    12481 cggggcgcgg gtctttctcg tcaatccccc ggtgatcaac atcggcggcc gcttcgccgc
    12541 cgccctttac cagttcacca tgcagtccac ggacatccag gccctctacg ccgccgcacc
    12601 cctgctcgag cgccggctcc gggccgaccc gatgttcaag gatgtcaaca gcgatctgcg
    12661 catcagcaat cccgaggtga tcgtcgccat cgaccgcgac cgtgctgcct cgctcggact
    12721 ctcggcgcag accatcgagg aggcgctcta ttccagttat ggcacccgcc aggtctcgac
    12781 tatcctggcg cccaacaacc agtactacgt catgctcgaa ctcctgcccg aataccagcg
    12841 cgacgcctcg gcctttcagt atctctatgt gcgctcgccg cagactgaca agctggtgcc
    12901 gctctcgacc ctggcgacga tctcccctgg ggccggaccc ctgacggtca accactccgg
    12961 gcagctgccc tcagtgacag tctccttcaa cctcgatgac ggggtggccc tgagcgaggg
    13021 cgtggcggcg gtcaatcggg cggcgcgcga gaccctgccc gcctcgatcg ccaccagttt
    13081 ttcgggcaca gcccaggcct tccagtcctc gcagagcagc ctgctcgccc tgctcttcct
    13141 ggccattctg gtcatctaca tgattctcgg catcctctat gagagtttta tccatccgct
    13201 caccatcctc agtggactgc ctttcgctgt tttcggggcg ctggtcaccc tgctgatctt
    13261 taaaatggac ctgaatcttt acagctttgt cggtctgatc atgctcatcg gcgtggttaa
    13321 aaagaacgcg atcatgatga tcgactttgc tatcacggcg cgtgatgaag ggctcggtgc
    13381 gcgtgaggcc atcctccgcg cttgcagcat ccgtttccgg ccgatcatga tgaccacagt
    13441 ggccgccctg atggcaaccc tgccgattgc gctgggaatg ggcgcggggg cggagtcgcg
    13501 ccgaccgctg ggcctggccg tggtgggcgg gctgcttttt tcgcagatga tcaccctcta
    13561 cgtcacgccg gtcttttata tctatctgga aaagctgcag aacaggctgg gcagagtttt
    13621 taacaagggt gtgagggctt gagcaggaga tgaagaaaaa aaagggcggc tgagaagatc
    13681 agccgccctt ttttaaatgc gaaggggaac gattacttgg ttgtcgctgc ctgggcttcg
    13741 gccttcatcc catcaccggc ggtgatggcg atctcaacgc ggcggttctg cgcgcggcct
    13801 tcgaccgtgg cgttgtccgc aatcggttga ctctcgccat agcccatgat ggtgaagcgg
    13861 ctgatatcga cgccactctt ggccaggaaa gaagagacgg cgtgagcgcg cgactcggag
    13921 agcttttgat tatagtcttc cgctccctgg gagtcggcat ggccctcgat gcggaggaca
    13981 gtgccgctgt acttgttcag agtcaccgcc agctgctcca gatcggcctg ggcggcgggc
    14041 ttcagcacag atttgccggt atcgaagagc aggccggatt tgagggtgac cttgatgcct
    14101 tcgccgacgc gttcgacttc agcatcggcg agttcttttt cgatttcagc agcctgctgg
    14161 tccattttgt tgccgatgta ggcgccggct gcaccaccga ttgccgcgcc gatgatggcg
    14221 ccgagggcgg tgttgccgac ctggttgccg atgacagcac cggccgcacc gccggcggca
    14281 gtgccaaccg ctgcgccctg ggtcgccttg gtcatgctgg cgcaggatat catgccgaca
    14341 gaaagaacag caactaatag gaccatcatt aatttgcgca tttcaacctc ctgtttccta
    14401 atgcgagtaa ctttcacgct gccccgttca ttaccccttg ctaggcaggg cagcggatta
    14461 cgggtataac ttacagagtt gtgatgaata agtcaataaa aatcgcatct tgaccggttc
    14521 cggccggcgc tgccgagctt tcagataccg gcgtataccc ggggtgcagt tcttcaacac
    14581 attttgccag tcttgagttc catcaatata aagagaacaa aatattctgg agtatattcc
    14641 gcgacatcac attgttgccg gaccccattg aaaagccctg aaagggattt aacctttcag
    14701 ggcttttttc tattgtgcgc gggccatggg cccgcacacg aatcggagct gagggctatt
    14761 taagcaggac catccgtttg atctcctgga agctgccggc ctggagtcgg tagaagtaga
    14821 tgccggaggg caccggttcg ccctgttcgt tggcgccgtt ccagaggacc tggtggctgc
    14881 cggatgcggc cgtgccgttg accagggtgc ggatcagccg gccctgaacg tcaaagatat
    14941 cgaggcgcag cgagccggcc tcatccattt caggcaggtt gtaggagatg gtcgtcgaag
    15001 cattgaaggg attgggatag ttctgtccga gttcgcaggt gagcggaatc tcctcctgtt
    15061 cgatcacctc ctccagcggt gcataggatt tttccagcac ggccagggcg gccatgcggg
    15121 ccggccggtc aatgctgatc cagagggggt cgctctcgtc ggtttctgtc attttgccga
    15181 ggcggccgtg gagcatattg gggccgttgt cgtgggcgac ctggccgccg ccttcatcaa
    15241 agctgtagta ggcgacgagc ccctcttcgg tgccgcgcgg ccggtcgaac atgcgggcgg
    15301 cgatttgctc gggggtgagg gccatgttcc agaagctcat ctcgtcgagc agccccttga
    15361 agtggtattc atagatattg ttatg
//
LOCUS       DAOPWU010000039        15381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_3915_length_15381_cov_4.313389, whole genome shotgun sequence.
ACCESSION   DAOPWU010000039 DAOPWU010000000
VERSION     DAOPWU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15381
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3915_length_15381_cov_4.313389"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKM23_03760"
     CDS             <1..367
                     /locus_tag="PKM23_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90623.1"
                     /translation="DNAPDADGDGVPNGQDPDYVKPGDGTGQAAGKGKAQGKGKGARF
                     HGFVDEDGDGINDNLRDGDGDGIANCQDKDWVRPMDGTGAKAGKGARGGRGFGRGNAG
                     SSSTTPGGNGPGNQGGKGK"
     gene            426..1592
                     /locus_tag="PKM23_03765"
     CDS             426..1592
                     /locus_tag="PKM23_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90624.1"
                     /translation="MRRRSYFSRDRTMMRNLYTLLILLLFVTAASGQISAALENQTGY
                     ISNAFSNHAALPDWYTALDGALNQDWTGERGGLRLHYDGSATLFRQYSERNHHSHEAG
                     LAAYLLMGESEHRLDAGLQAGGRFHSESYQWYEQEQLQAYASLKFIAAPQWYTYAGVS
                     WNLRRYPELTPFSHQRTQLYGRSSWFLPTGTTLIAEADLLFKSYTSAENEAQAAYSDV
                     ATSGEGNSSQLLMLVRAAQALSAKSGLSLEYQLRHNLASSTRYLAGADSLYYSDEELF
                     EDFFAWHGTAVQMSLRQELPWGMRLTLSGRRELRSFDDRPAADLLGEPFADGRLREDR
                     RSTLAIDFEKKVRLDEEGSALLIALNGAWLQNASNDLYYDYRNSWWSLGFSWDF"
     gene            complement(1594..>2340)
                     /locus_tag="PKM23_03770"
     CDS             complement(1594..>2340)
                     /locus_tag="PKM23_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY90625.1"
                     /translation="ALPETLAESELFGHEKGAFTGADRMRPGRFEAADGGTLFIDEVG
                     DMPPAIQAKLLRVLQEREVERLGSSRPVQVDVRIIAATNRSLAEMVKDKTFREDLYYR
                     LNVVRVELPPLRERKEDIPLLADHFMRRFAVENGRAMQGCTREAMDALLKHPFPGNVR
                     ELQHLIEQAVVLSRDEWIGLEDLGLAGAQAPSSESASTGTFTERVEAFEKRLIQQAMA
                     EGHGVQSQAARLLGISERHLRYKLTKYGMK"
     assembly_gap    2341..2440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2441..2769)
                     /locus_tag="PKM23_03775"
     CDS             complement(<2441..2769)
                     /locus_tag="PKM23_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY90626.1"
                     /translation="MAISILVVDDEPSQRSALAGFLRKKGYVTVEAESAAAALTVARQ
                     QVVDLVLTDLRMPGMDGARLLQELKSLNPLLDVIIMTAFGTMSEAVGAMKEGALDFIT
                     KPIDLEQL"
     gene            complement(2811..4460)
                     /locus_tag="PKM23_03780"
     CDS             complement(2811..4460)
                     /locus_tag="PKM23_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY90627.1"
                     /translation="MKREHLYTTRWIAALALVLAAMVLVWALLELRSTRLELTEALEE
                     QAQTVMALARRGLDNAALSWEFVESGLEERLLDNARLLERLDREGRLTPATLEQIARE
                     NDLHRINLFDREGRRLLSSHSFVGEPPVQAPRRLLQPVLGDSSDELVLGFRQRFFGAG
                     QFFAVARHRRGGGAIVVHADAEALLAFRRDIGAGSFIKLIADNETIRYAVVQDSGGVL
                     LASDGVTEMSAPAADPFLGRVLSADPPQMRFTRYRDEEILEIAAPVHLGENDATLLRI
                     GLSAASLHRAERTALWRIGISTLLLLLTGFLTTGALLRELRLHRRAEEQRERQEQIDA
                     MGHLASGVAHEIRNPLNAVSIIAQRLDREFEPSQESEEYHQLTGTLAAESRRINDIVS
                     QFLRFARPAPLNRQPVRLDALVAHVAALLQGEAADSGVTLISQCREVKEVMADGDQLT
                     QALINLARNSLAACAKGGRIELTCTPHGDRILLSVTDDGAGIDPENLGKIFNLYFTTR
                     EGGSGLGLSIVQQIISQHDGTIEVESAPGRGTRFTITLPAR"
     gene            4775..9676
                     /locus_tag="PKM23_03785"
     CDS             4775..9676
                     /locus_tag="PKM23_03785"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HNY90628.1"
                     /translation="MGLDPVPESIHAVRFDPSYYYNAPVRVNKLAQQLAEAWQAHGVN
                     VVFYKAYDPVHGAKYKTRYALNEMADYGRQDMLKYILKECSTRGIQVMAWLPAFQHKQ
                     AWEKHPEWRLRNPDGSEYHLNEQSYPLCAANPEVRTWWVGLIRDLLEDYDGLAGIDIG
                     EPVVRWQGAYCGCASCQLQIRASGFGDASALGLTKSLLASIEAIHEAHKLACITSVVS
                     PKPDGSLFTTTEQRGYTGFDLEALLEAPHKPDWVSLELMWQQWADLYKHSACFNPAWV
                     EGAAATVREQVAGRSRLLVHLELTPFGQQGVDGGKLQESMRAARRADLFDIDLYDTHQ
                     IDLEKVWPDVAAGFAWQPARKVLVVSDPIGENDARQVASLLSHFKTETEILLLDADQP
                     YTPGRLAGRDLLFYIGVDPHFILPTAFLQEAAGFKGTLAWIHFGLGQYLEQPGLPDHG
                     IRYSGSMADSAWNRVRYKQALLPRQDPDLDRAVLTDSAHVRVLAWMEKGAERLPYALH
                     SRNLWYFADLTTAFVREGGSYIVFADLLHDILRQPHQTRRLAMVRIEDISPLSDAASL
                     RRIADYLHGEKVPFAISLVPFYLDPASNTAASLSDQPEFVKAIRYMVRKGGVIVMHGN
                     THQYRGETTADYEFWDMMHNEPLFSDSREYVRERIETGLQEMARNGLWPVTWETPHYG
                     ASQLAYSVINNYFSSAYERRQTIDLHGSDQLLPWLIYEHTAGAKIIPENLGYIPLSDQ
                     RSGPLLEAARNNLALRDGVASFFFHPFCDIAVLKEIVPGLKKMGYRFTDPRHTSQQVR
                     GPGFRVVTGSGQIELPLNSAWLREFYIDEQGSIRDERYSDALRSDTLRKSVVVPEGWI
                     YVAAAAPVRPQGALANLAAGFARHVPRLGEYIGAVKPLAVPDEPPARMVLLRHSDAPA
                     RLLRDQDNWEQALGSVGIDGTRLDVGQFFTVPEGINLIVVPAAAALALNQQQILLLVR
                     SVQNGANIIIERPSQLSAAIGILPLEESRLMKGVTDEYYPTVPISWAAPDTLSDFETE
                     FDYITYASDPASGQPAVIGGEYGEGRYLYWGTLFDPETRGGYGRYPFLPEMLRYQFGL
                     KSLVRREAVEIFFDPGSREEISIEDLIKMWRKNGVRTIHAAGWHSYRDYTYDYGRLIE
                     LAHQNAMRVYLWLELPHVSDRFWLDHPEWREKNALGADADTSGAHWRRPMALNLAACR
                     EAVMAELRSLLLEHPWDGVNLAELYYESAAGPAQPRWFTPMNQGFRENFLQRHGYDPR
                     LLFDPSSSRYWQRDARALQALYVLREELVNEQIRRFVTLFRQVEEEKKTSWEIIVTTL
                     DAAMAPAAAAMGVNTAAIAALADELPFTLQLEEPPLANPSEPEEYTAMIDRGAAYSRG
                     RIPSATLNLVDRPQQPGLVTRQITGLELYRTLQQGQERGARLSLNSEASLYEVDSGYL
                     PLALAAATRESLSVSQWRIDAATSVSLLLDPKQHPDVLVDGSVWPAYHRGHLILPAGH
                     HTIEPLDRTSSFFKGFWAEGRLIALSGELLELQRIVRGIRLRYRSETANWMVLSEKPH
                     SIEVDGIKSAPEMMRGELGYAFALPAGEHRAEIYTTSRTTQLVKQISLALSGLIAGGG
                     LLFGIVLGFLYFKNARRHRKSTA"
     gene            9779..11071
                     /locus_tag="PKM23_03790"
     CDS             9779..11071
                     /locus_tag="PKM23_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90629.1"
                     /translation="MLPLMHPALDLLFLGSVVIIWGMIVYQLVLTLAGYRYRHRIQQE
                     HQQLLAKGGEPAPVSIMIPARNEEVVIAKTMRTILALDYPADKLELVIIDDGSTDRTA
                     EIITARAQMDPRIKLVRLPTGETGHGKAYALNEGLKHCTHDLIAIYDADNTPEPSALR
                     HLALHLQADARLAAVIGKFRCVNRQRTWLTRFVNIETIAFQWILQAGRFHLSRVAILP
                     GTNYIIRKSVITAVGGWDDRAITEDSELSMRIYQAGWQIKFMPLSVTWEQEPEILRVW
                     SRQRTRWVRGNNYVIRKFTGPMLKTRSRFLVTEFIYLFALYYLFLAATVVSLLLFFAS
                     STGLVALNVPGPYFAVWVSAFALFTLEIFVVLSYEGEDSLANILLTIAMYFTYCQLWL
                     YIVFKAIILDVRRHRVGVWDKTERFKVEETAEEGATAG"
     gene            11068..11949
                     /locus_tag="PKM23_03795"
     CDS             11068..11949
                     /locus_tag="PKM23_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90630.1"
                     /translation="MRLCKRDIAPFLHDPARAAGRTAFCVALLWTILSGIFTESAAQE
                     TGLRFFAEAYDETIYQRREGRNTVTMAQLYEGVRKPVPGVGIWDSYVKIRYGSDANKD
                     FWNNRGEVMLGTRLRFFEKVYLALYGEYVRGEYIAYDQEAMPYAPSYEDWRGGLIFWH
                     GWDPGGWAPGGALPFTAWSETYADINYLEKDDQNIIGYIESKLGLQLLRLHKTRLDLY
                     GVVYAYYDRNADFWNNHAAAAPGIRFTPWSDLDLNFRVEYLWGTYYQRSGRYENPYER
                     NYQDLRIGFFFWYGWGD"
     gene            11961..13790
                     /locus_tag="PKM23_03800"
     CDS             11961..13790
                     /locus_tag="PKM23_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY90631.1"
                     /translation="MKERISIFFLLAALVAGMAIAQTIPAQLLWDYSTGGPVTAVAPF
                     PDVNGDGRPDVLAGSADNLVYCLSGGGPDYAREIWSCRTLGAITDVELIGDVNADGYP
                     DAVAGSRDNIVYCISGKPADEGLELWQRADSATIHSVAALGDVNGDQIGDVVVGTAMN
                     NMICISGAAAQQGKILWQYGSDADFWNVIAMPDLNGDGKKECAGSCDNFIYCFSGAGV
                     TQGQQGGSAAKAIWAAPYDAGARVWSIASITDVNGDGKADLLLGSNMDRVECLSGATG
                     KNIWSFSTGADAICVAVIGDLNGDSKSDVLAGSADDYGYALSGATGQQIWRVKFGSTV
                     LSAAAIGDINNDGYPEAVFGSDADEVFCISGGGATKGQKLWSYTATGGIGSLAAIGDV
                     NLNGITDVAATSADSYLRLFEGNSRILDVELVSFTAAVQSDGVLLSWRTASESNNLGF
                     EVQRSFDGHSYELLGFVPGNGTTAQQKEYSWFDTLSSWDKLYYRLKQVDRDGQCEYFP
                     AMKVVQKLPARLTLLGNYPNPFNAGTFIRYDLPAAEEVTVTIYNLRGEKVRELWHGVQ
                     PAGSHNLNWDGSRMTGEAVASGAYLCIITAGEEIARLRITCLK"
     gene            complement(13931..14665)
                     /locus_tag="PKM23_03805"
     CDS             complement(13931..14665)
                     /locus_tag="PKM23_03805"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY90632.1"
                     /translation="MFQHIFGPVHSRRLGTSLGIDLVPLKTCNLNCIYCECGRTTHLT
                     MERKEYVPFDEVCEELTRFFIANPTLDYLTFSGSGEPLLYSRAGELIEWIKERAPDIP
                     LAILTNGTLFTDPAVRREVLRADLILPSLDAASPALYRKVDHPHPILQIEKIIAGLVE
                     LRREYGGRIWLEVFIVPGFTDQESELSALREAILRIAPDRVQLNSLDRPGTLPNLRAA
                     SRDELERIISFWQLPNVEIVKRPASG"
     gene            complement(14716..>15381)
                     /gene="wecB"
                     /locus_tag="PKM23_03810"
     CDS             complement(14716..>15381)
                     /gene="wecB"
                     /locus_tag="PKM23_03810"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNY90633.1"
                     /translation="TERARQALLAEGFPEAGIHVTGNTIVDALLEISQRSFDWRKSAL
                     DVIPQEGHMVLITAHRRESFGEPFRELCQAIRELAGLFHDTHFVYPVHLNPNVRRPVG
                     EILHGLGNVHLIEPVDYLTLVQLMKRSVLILTDSGGIQEEAPNFAVPVLVMRDTTERP
                     EGVEAGVARLVGTRQEKIVAEAAAVLKNGSWIDSSHGTVNPYGDGLAARRIVEILLGT
                     ALT"
BASE COUNT         2990 a         4840 c         4559 g         2892 t
ORIGIN      
        1 tgacaacgcc cctgatgctg atggcgacgg cgtccccaac ggccaggatc ccgattatgt
       61 aaagccgggc gacggcaccg gtcaggccgc aggcaagggc aaggcccagg gcaaaggcaa
      121 gggcgcccgc ttccacggct ttgtcgatga ggacggcgac ggtatcaatg acaatctccg
      181 cgatggcgac ggcgacggaa tcgccaactg ccaggacaag gactgggtgc ggccgatgga
      241 cggcaccggc gccaaggcgg gcaagggtgc tcgcggcggc cgcggcttcg gcagaggaaa
      301 tgccggcagc agctccacca ccccgggcgg gaatggcccc ggcaatcagg gcggcaaggg
      361 caaatagatc cgcctgatcc tgcagccttc aaccagaaca aaataaccag aagcggcggc
      421 gcaaattgcg ccgccgctcc tatttcagca gagaccggac catgatgcgc aacctctata
      481 ccctccttat tctcctatta tttgtcacgg cagccagtgg acaaatctcc gccgccctcg
      541 aaaaccagac cggatatatc agcaacgcct tctccaacca tgccgccctg cccgattggt
      601 ataccgccct ggacggcgcc ctgaaccagg actggacagg tgaacgcggc ggcctgcgtc
      661 ttcactacga cggcagcgcc accctcttcc ggcagtacag cgagagaaac caccacagcc
      721 atgaggcggg gctggccgcc tatctgctca tgggtgagtc ggaacaccgc ctggatgccg
      781 gactgcaggc cggggggcgc tttcacagcg aaagttatca atggtatgaa caggaacagc
      841 tccaggccta tgcctcgcta aaatttatcg ccgcaccgca gtggtatacc tatgccggag
      901 tctcctggaa tctgcgccgc tatcccgaac tgaccccctt ttcccaccag cggacccagc
      961 tctacggccg cagcagctgg ttcctgccga caggcactac cctgatcgcc gaggccgacc
     1021 tgcttttcaa gagctatacc agcgcagaaa atgaagctca agccgcctac agcgatgtcg
     1081 cgaccagcgg cgagggcaac agcagccagc tacttatgct ggtgcgcgcc gcccaggcgc
     1141 tgagcgcgaa aagcggacta agtctggaat accagctgcg ccacaacctt gccagcagta
     1201 cgcgctatct ggccggtgcc gacagtctct attattcgga tgaggagtta ttcgaggatt
     1261 tttttgcctg gcacggcact gccgtgcaga tgtcactgcg gcaggaactg ccctggggga
     1321 tgcgcctgac cctcagcggc cgccgcgagc tgcgcagctt tgatgaccgg ccggcggcgg
     1381 atctgctcgg cgagcccttt gcggacggtc gtctgcgcga ggaccggcga tcgaccctgg
     1441 cgatcgattt tgaaaaaaag gtcaggctgg acgaggaagg gagcgccctg ctgatcgccc
     1501 tcaacggggc ctggctgcag aacgcctcca acgatctcta ttacgattac cggaacagct
     1561 ggtggagcct gggattctcc tgggactttt gagctatttc atgccatatt ttgtcagttt
     1621 gtagcgcagg tggcgctcgc tgatgccgag caggcgcgcc gcctgactct ggactccgtg
     1681 cccctccgcc atggcctgct ggatcaaccg cttttcaaag gcctccaccc tttcggtgaa
     1741 ggtacccgtc gaagcgcttt cggaggatgg agcctgcgcc ccggcaagcc cgagatcctc
     1801 caatccgatc cactcgtccc ggctcagaac caccgcctgt tcgatcagat gctgcagttc
     1861 gcgcacattg cccggaaagg ggtgtttgag cagggcgtcc atcgcttcac gggtgcagcc
     1921 ctgcatggcg cggccgttct cgacggcgaa acggcgcata aaatggtcag cgagcagggg
     1981 gatgtcttcc ttgcgttcgc gcaggggcgg cagctccacc cgcacgacat tgaggcggta
     2041 atagagatcc tcgcgaaagg ttttgtcctt gaccatctca gccagggaac ggttggtggc
     2101 cgcgatgatg cgcacatcca cctgcaccgg ccttgaggag ccgaggcgct cgacctcccg
     2161 ttcctggaga acgcgcagca gcttggcctg gatcgccggc ggcatgtcgc cgacctcatc
     2221 gatgaagagg gtgccgccgt cagccgcctc gaagcggccg gggcgcatcc ggtctgcccc
     2281 ggtaaaggcc cccttctcat ggccgaagag ctcgctctcg gccagggtct cgggcagggc
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agctgctcca gatcgatggg
     2461 cttggtgata aaatcgaggg cgccctcctt catcgccccg accgcctcgg acatggtgcc
     2521 aaaggcggtc atgatgatga catcgagcag ggggttgagg gactttagtt cctgcaggag
     2581 gcgggcgccg tccatgccgg gcatgcgcag gtcggtcaga accagatcga ccacctgctg
     2641 ccgcgccacc gtcaaggccg cagccgctga ttcggcctcc accgtcacat agcccttttt
     2701 gcgcaaaaag ccggccagag cgctgcgctg gctgggttca tcatcgacga cgagaatgga
     2761 aatggccatg ctctcctctc ttcagtgtac gctctccggc aggccaggat tcagcgcgcc
     2821 ggtagtgtga tggtgaagcg ggtgccgcgt ccgggcgcgc tctcgacttc tatagtccca
     2881 tcatgctggg aaatgatctg ctggacaatg ctgaggccga gaccgctgcc cccctcgcgg
     2941 gtggtgaagt aaagattgaa aatcttgccg aggttctccg gatcgattcc ggcgccgtcg
     3001 tcagtgacgc tgaggaggat gcgatctccg tggggtgtac atgtgagttc gattcgcccg
     3061 cctttggcac aggccgccag gctgttgcgg gccagattga tcagggcctg ggtcagttgg
     3121 tcgccatcag ccatcacctc cttcacctcc cggcactggc tgatgagggt gacgccggaa
     3181 tcggccgcct ccccctgcag cagggcggcg acatgggcga ccagggcatc gagacggacc
     3241 ggctggcggt tgaggggggc gggccgggcg aaccggagaa actggctgac gatgtcgttg
     3301 atgcgccggc tctcagcagc cagcgttccg gtgagctggt ggtactcttc actctcctgg
     3361 gagggctcga attcacgatc gagccgctgg gcgataatgc tgacggcgtt gagggggttg
     3421 cggatctcat gggcgacccc cgaagcgaga tggcccatgg cgtcgatctg ctcctggcgc
     3481 tcgcgttgtt cttcagcgcg gcggtgcagc cgcagctccc ggagcagcgc tccggtggtg
     3541 agaaaaccgg tcaggagcag cagcagtgtc gaaatcccaa tcctccacag ggcggtgcgc
     3601 tcggcccgat ggagcgatgc agcagagagg ccgatgcgga gcagggtcgc gtcattctcg
     3661 cccagatgca cgggtgcggc gatttcaagg atctcctcgt cccgatagcg ggtgaagcgc
     3721 atttgcggag ggtccgcgct cagtacacga ccgagaaagg ggtccgcagc cggggcgctc
     3781 atttcagtca ccccatccga ggcgagcagg acgccgccgg aatcctggac cacagcgtag
     3841 cggatggttt cgttatccgc gatcaacttg atgaatgagc ccgcaccgat atcgcggcgg
     3901 aaggcgagga gcgcctccgc atcggcgtgg acgacgatgg cgccgccgcc gcggcggtgc
     3961 ctggccacgg cgaaaaactg gccggcgccg aagaagcgct gccggaagcc gagcaccagt
     4021 tcgtcgctgc tgtcacccag caccggctgc agcagccggc gcggcgcctg caccggtggc
     4081 tccccgacaa aggagtggct ggagagaagg cgccgcccct cacggtcgaa gaggttgatg
     4141 cgatggagat cgttctcccg ggcgatctgc tcgagggtcg cgggcgtgag tctcccctcg
     4201 cggtcaagcc tttccagcag ccgtgcatta tccaaaagcc gttcctcgag ccccgattcg
     4261 acaaattccc aggagagggc ggcgttgtcg agcccgcgcc tggccagggc catcaccgtc
     4321 tgtgcctgct cctcgagggc ttcagtcaac tccagccgcg tgctgcgcag ctcgagcagg
     4381 gcccagacca gcaccatcgc cgccagcacg agggccagcg ctgcaatcca cctggtcgtg
     4441 taaagatgtt cccttttcat caagcctgcc ttccaaggtt gcagcgtgtt caaacaaggc
     4501 tgcagcaggg ggatcagcag ccattcatct acagatataa tctatgatta ttttctccat
     4561 taacaagcag aaatgaaagt gagagacggc gcatttcaca aggaaagtga atgcggtttc
     4621 ctgcgcatac ggtacgaatc ttgcagacga ttgtttttat ttaacattga cggaaaacag
     4681 gaggtcagga ttgaaacggc cgctgctctt aatcattgtt ttattgtata ttatcgctcc
     4741 agctaaattc ccggccctgg cacaaaattc acccttgggc ctcgatcctg ttccggaatc
     4801 gatccatgcg gtgcgttttg atccatccta ttactacaac gccccggttc gcgtcaacaa
     4861 gctggcacag cagctggcag aagcatggca ggcgcacggc gtcaacgtcg tcttttacaa
     4921 ggcctacgat cccgttcacg gcgccaagta caagacccga tatgccctca atgagatggc
     4981 cgactacggc cgccaggata tgctcaaata tattctcaaa gaatgcagca cgcggggaat
     5041 ccaggtgatg gcctggctgc cagcctttca gcacaagcaa gcctgggaga aacatcccga
     5101 gtggcggctc cggaatcctg acggcagcga ataccacctg aacgaacaga gctaccccct
     5161 ttgcgcggcc aatcctgagg tacgcacctg gtgggtgggg ctgatccgcg atctgctcga
     5221 agactatgac ggcctggcgg gaatcgacat cggtgagccg gtggtgcgct ggcagggcgc
     5281 ctactgcggc tgcgcctcct gtcagctgca gatcagggcg tcgggttttg gcgacgcttc
     5341 cgccttgggg ttgaccaaaa gcctgctcgc cagtatcgag gccatacatg aggcacacaa
     5401 actggcttgt atcacctctg tggtcagccc caaacccgac gggagccttt tcaccacgac
     5461 agaacaacgc ggctataccg gctttgatct cgaggccctg ctcgaagctc ctcacaaacc
     5521 ggactgggtc agtctggagc tgatgtggca gcagtgggcc gatctctaca aacattctgc
     5581 ctgcttcaat ccggcctggg tggagggagc cgccgcaacc gttcgggaac aggtggcagg
     5641 gcgcagccgc ttgctggttc acctcgaact cacccccttc ggccagcagg gcgtagacgg
     5701 cggcaaactg caggagagca tgcgggccgc caggcgggcg gatctcttcg acatcgatct
     5761 ctatgacacc caccagatcg atctcgaaaa agtctggccc gacgtagcgg cgggttttgc
     5821 ctggcagccg gcgcgcaagg ttctggtcgt gagcgatccc atcggcgaaa acgatgcccg
     5881 gcaggtggcc agcctgctca gccatttcaa aacggagacc gaaatactcc tcctcgatgc
     5941 agaccagccc tacacgccgg gccggctggc cggccgcgat ctgctctttt atatcggggt
     6001 cgacccccat tttattctcc ccacggcctt tctccaggag gccgccggat tcaaaggcac
     6061 cctggcctgg atccacttcg gccttgggca gtatctggag cagcccggcc tgccggatca
     6121 tggcatccgc tattcaggca gtatggctga ttcagcatgg aaccgggtgc gctacaagca
     6181 agcgctgctg ccgcggcagg atcctgattt ggaccgggcc gtcctcaccg acagcgcgca
     6241 cgtccgggtg ctggcctgga tggaaaaagg cgcagaacgt ctgccctatg ccctccacag
     6301 tcgcaacctc tggtacttcg ccgacctcac caccgctttt gtgcgcgaag ggggcagcta
     6361 tatcgtattc gccgatctgc ttcacgatat cctccggcag ccgcaccaga cccgccgact
     6421 ggcgatggtg cgcatcgagg acatctctcc cctttccgac gccgcctccc tgcgccgcat
     6481 cgccgactat ctccacggtg aaaaggtgcc ctttgccatc tcgctggtcc ccttttatct
     6541 cgacccggcc agcaataccg ccgcctctct ctccgaccaa cccgaatttg tcaaggccat
     6601 ccgctatatg gtgcgcaagg gtggggtcat tgtcatgcac ggcaataccc atcagtaccg
     6661 cggcgagacc acggccgact atgaattctg ggacatgatg cacaatgagc cgctcttttc
     6721 cgattcccgc gagtatgtcc gtgagcgcat cgaaaccggc ctgcaggaga tggcccgcaa
     6781 cgggctttgg ccggtcacct gggagactcc ccactatggc gcctcgcagc tcgcctactc
     6841 ggtaatcaac aactatttca gctccgccta tgagcgccgt cagaccatcg atctgcacgg
     6901 ctccgatcag ctcctgccct ggctcattta cgagcacacg gcgggggcca aaatcatccc
     6961 ggagaacctc ggctacattc ccctgagcga ccagcgctcc ggaccgctgc tcgaggcggc
     7021 gcgcaacaat ctcgccctgc gcgatggcgt cgccagcttc ttttttcatc ccttctgcga
     7081 catcgcagtg ctcaaggaga tcgtcccggg cctgaaaaag atgggctatc gcttcacaga
     7141 tccccgccat accagccagc aggtccgagg ccccggcttc cgggtcgtga ccggcagcgg
     7201 ccagatcgaa ctgcccctca actccgcctg gctgcgtgaa ttttacatcg atgagcaagg
     7261 ttccatccgc gatgagcgct attccgatgc cctgcgcagc gacacccttc gcaagagcgt
     7321 ggttgtgccg gaggggtgga tctatgtggc cgcggcggcg ccggttcgac ctcaaggcgc
     7381 cctggccaat ctggccgccg gcttcgcccg gcatgtgccg aggctgggcg agtatatcgg
     7441 cgctgtcaaa cccctggccg tgcccgatga acctcccgca cgcatggtgc tgctgcgcca
     7501 cagcgatgcc ccggcgcggc tgctgcgcga ccaggataac tgggagcagg cgctgggcag
     7561 cgtgggcatc gacggcacga ggctggatgt ggggcagttt ttcactgtcc cagagggaat
     7621 caacctcatc gtcgttccgg ctgcagcggc tctcgccctc aaccagcagc agatcctcct
     7681 gctggtgcgc agcgtccaga acggcgccaa catcatcatc gagcgtcctt cgcagctcag
     7741 cgctgctatc ggcatcctgc cgctcgagga gagccgcctg atgaaagggg tgacggatga
     7801 atactacccc accgtgccca tcagctgggc cgcgccggac accctgagtg actttgagac
     7861 cgagttcgat tatatcacct acgcctccga tccggcctcc ggccagccgg cggtgatcgg
     7921 cggcgaatac ggcgaaggcc gctatctcta ctgggggacc ctcttcgatc ccgagacccg
     7981 gggcggttac ggccgttatc ccttcctgcc ggagatgctg cgctaccagt tcggcctcaa
     8041 atccctggtc cgccgcgagg ccgtcgagat ctttttcgat cccggctcgc gggaagagat
     8101 cagcatcgag gacctcatca agatgtggcg caagaacggg gtgcgcacca ttcatgccgc
     8161 cggctggcac agttaccgcg actataccta tgattatggc cgtctgatcg aactcgcgca
     8221 ccagaacgcc atgcgcgtct atctctggct cgagctgccc catgtctcgg accggttctg
     8281 gctcgatcat ccggaatggc gggagaagaa cgccctcggg gcggacgccg acacctcggg
     8341 ggcgcactgg cggcgtccga tggcgctcaa cctggccgcc tgccgcgagg cagtgatggc
     8401 cgagctgcgc agcctgctgc tggagcaccc gtgggatggc gtcaacctgg ccgaactcta
     8461 ttatgaatcg gcggccggcc cggcgcagcc gcgctggttc acgccgatga accagggctt
     8521 tcgcgagaat tttttgcagc gccacggtta cgatcccagg ctcctcttcg atccatcctc
     8581 cagccgttac tggcagcgcg acgcccgggc cctccaggca ttatacgtcc tgcgcgagga
     8641 gctggtgaat gaacaaatcc gccgctttgt cactctcttt cggcaggtgg aggaggagaa
     8701 aaagacctcc tgggagatca tcgtgaccac cctggatgcc gccatggcgc cggcggcggc
     8761 ggccatgggc gtcaacaccg ctgccatcgc cgccctcgcc gatgagctcc ccttcaccct
     8821 gcagctcgag gagccgcccc tggccaatcc atcagagccg gaagaataca cggccatgat
     8881 cgaccgcgga gcagcctaca gccgcggccg aattccgtcg gccaccctca acctcgtcga
     8941 ccgcccgcag cagcccgggc tggtgacccg tcagatcacc gggctcgagc tctaccgcac
     9001 cctgcagcag gggcaggagc ggggcgcccg cctatctctc aactcggagg ccagccttta
     9061 cgaagtggac agcggctacc tgccgctggc cctggccgcg gcgacacggg agtccctctc
     9121 tgtttcgcag tggcgcatcg acgccgccac ttccgtcagc ctgctgctcg atcccaaaca
     9181 gcacccggat gtgctggtgg atggttcggt atggccggcc taccaccgtg gccacctgat
     9241 tctccccgcc ggccatcata ccattgagcc cctcgaccgc accagttcct ttttcaaggg
     9301 cttttgggcc gaaggccgac tgatcgccct ctccggcgaa ctgctcgaac tacagcggat
     9361 cgtgcgcggc atccgcctgc gataccgttc ggagacagcc aactggatgg tgctgagcga
     9421 aaaaccgcat tcaatcgagg tcgatggcat caaatcagca ccggagatga tgcgcggcga
     9481 acttggctat gcctttgccc tgcccgccgg ggagcaccgg gcggaaattt ataccaccag
     9541 ccggaccacg cagctggtga aacagatctc gctggccctc tccggcctca tcgccggggg
     9601 cggcctcctc ttcgggatcg ttctcggttt tctctatttc aaaaacgcca ggcgccaccg
     9661 caagagcact gcctgaaaag gctccagatc actggacggg gccgcccccg tccacatcat
     9721 caattcacta caccagaccg tcgcaccaaa tctcttcgct ctgaattgga ggtatcctgt
     9781 gctgcccctc atgcaccctg cgctcgatct gctcttcctc ggcagtgtcg tcatcatctg
     9841 gggaatgatc gtctaccagc tggtgctcac cctggcgggc tatcgctatc gccaccgcat
     9901 ccagcaggaa caccagcagc tgctggcaaa aggcggcgag ccggcgccgg tctcgatcat
     9961 gatcccggcg cgcaacgaag aggtggtcat cgccaagacc atgcgcacca tcctcgctct
    10021 cgactatccg gccgataaac tcgagctggt gatcatcgac gacggttcca cagaccgcac
    10081 cgctgagatc atcacggcgc gcgcccaaat ggatccccgt atcaagctgg tccgtctgcc
    10141 gacgggtgag accgggcacg gcaaggccta tgccctcaat gaagggctca agcactgcac
    10201 ccacgatctc attgccatct acgacgccga caataccccg gagccctccg ccctgcgcca
    10261 cctcgccctt cacctccagg cggacgccag actggcggcc gtgatcggca agttccgctg
    10321 cgtcaaccgc cagcgcacct ggctgacccg cttcgtcaat atcgaaacca tcgccttcca
    10381 gtggatactg caggcgggtc gcttccatct ttcacgtgtg gccatcctgc ccggcaccaa
    10441 ctacatcatc aggaaatcgg tgattacggc ggtcggcggc tgggatgacc gcgccatcac
    10501 cgaggattct gaactgagca tgcgcatcta tcaggcgggc tggcagatca aattcatgcc
    10561 cctcagcgtc acctgggagc aggagcccga aatcctgcgg gtctggagcc gccagcgcac
    10621 ccgctgggtg cgcggcaata actatgtgat tcgtaaattc accggcccga tgctcaaaac
    10681 tcgcagccgc ttcctggtca ccgagttcat ctacctcttt gccctctact atctctttct
    10741 ggcggcgacc gtagtctcgc tgcttctttt cttcgccagc agcaccggcc tggtcgccct
    10801 caatgtgcct gggccttact ttgcggtctg ggtctcagcc tttgccctct tcaccctgga
    10861 aatctttgtt gttctcagtt atgaggggga ggattcgctc gccaatatcc tgctcacgat
    10921 cgcgatgtac ttcacctact gccagctttg gctctacatc gtcttcaagg ccatcatcct
    10981 cgatgtccgc cgtcaccgcg tcggggtatg ggacaagacc gagcgtttca aggtggagga
    11041 gacagcggaa gagggagcga cggccggatg agactttgca aaagggatat tgcgccattt
    11101 cttcatgatc cagctcgcgc agcagggcgc accgccttct gcgtcgccct gctgtggacg
    11161 atcctctccg gcatttttac agagtcggcc gcacaggaga ccgggctgcg tttcttcgcc
    11221 gaggcctacg acgaaaccat ctatcagcgc cgtgaggggc gtaacacggt caccatggcg
    11281 cagctctacg aaggagtgcg caagccggtg cccggggtgg gtatctggga cagctatgtc
    11341 aagatccgct acggcagtga cgccaacaag gatttctgga acaaccgcgg cgaggtaatg
    11401 ctcggcacca gattgcgctt ttttgaaaag gtctacctcg cgctgtacgg ggagtatgtc
    11461 cgcggagagt acattgccta tgaccaggag gccatgccct acgccccctc atatgaagac
    11521 tggcgcggtg gcctgatctt ctggcacggc tgggatcccg gtggctgggc gcccgggggt
    11581 gcgctcccct tcaccgcctg gagtgaaacc tacgccgaca tcaactatct tgaaaaggat
    11641 gatcagaaca ttatcggcta cattgaaagc aagctggggc tgcagctgct gcgcctgcat
    11701 aaaacccgtc ttgacctcta tggcgtcgtc tacgcctatt atgaccgcaa cgccgacttt
    11761 tggaataacc atgccgctgc agcgccgggt attcgcttca ccccctggag cgacctcgac
    11821 ctcaatttcc gtgtggaata tctttggggg acatactatc aacgttcagg ccgctacgaa
    11881 aatccctatg agcgaaatta tcaggatctg cgcatcggct tttttttctg gtatggctgg
    11941 ggagattaag gagaagaacg atgaaagaaa gaataagcat cttttttttg ctggcggccc
    12001 tggtcgccgg catggcgatt gcccagacca tcccggccca gctgctctgg gactattcga
    12061 cgggcggccc ggtgaccgcg gtcgccccct ttcccgatgt caacggcgac ggccggcctg
    12121 atgtgctggc cgggtccgcc gacaacctgg tctactgcct ttccggcggc ggtccagact
    12181 atgcgcggga gatctggtcc tgccggaccc tgggagccat caccgatgtg gagctgatcg
    12241 gggatgtcaa cgcggacggc taccctgacg ccgtggcggg atccagggac aacattgtct
    12301 actgcatatc cggcaagccg gcggacgaag gacttgaact ctggcagagg gcggactctg
    12361 cgacgatcca ttcggtcgcc gcactggggg atgtcaacgg cgaccagatc ggcgatgtgg
    12421 tggtgggcac cgccatgaac aacatgatct gcatctccgg cgcggccgcg cagcagggca
    12481 agatcctctg gcagtacggc agcgatgccg atttctggaa cgtcatcgcc atgcccgacc
    12541 tcaatggcga cggcaagaag gagtgcgccg gctcctgcga caattttatc tactgcttct
    12601 cgggtgcggg cgtcacccag ggtcagcagg gcggctcagc cgccaaagcg atctgggccg
    12661 ccccctacga cgcgggcgcg cgggtctggt cgatagcatc catcacggat gtgaacggag
    12721 acggcaaggc cgacctcctg ctcggcagca acatggaccg ggtcgagtgc ctctccggag
    12781 cgacgggcaa aaacatctgg agcttttcca ccggcgctga cgccatctgc gtcgccgtaa
    12841 tcggcgacct caacggcgac agcaagtccg atgtactggc aggatcggcc gacgactatg
    12901 gctacgccct ctccggcgct accggccagc agatctggcg ggtgaaattc ggctcgacgg
    12961 tattatcggc agcagcgatc ggcgacatca acaacgacgg ctatcccgag gcggtttttg
    13021 gctccgacgc cgatgaggtg ttctgcatca gcggcggggg cgccaccaag gggcaaaagc
    13081 tgtggagcta taccgctacc gggggtatcg gcagtctcgc cgccatcggt gatgttaatc
    13141 tcaacggcat caccgatgtg gccgcaacct ccgccgacag ctacctccgc ctcttcgagg
    13201 gcaacagcag aatcctggac gtggaactgg tctccttcac agccgcggtg cagtcggatg
    13261 gcgtgctgct ctcctggcgc accgccagcg agagcaacaa tctcggtttt gaggtgcagc
    13321 gctcctttga cggccactcc tacgagctgc tgggatttgt gcctggcaat ggcacaaccg
    13381 cccagcagaa ggagtacagc tggttcgaca cgctttcctc ctgggataag ctctattacc
    13441 ggctcaagca ggtcgaccgc gacggccagt gcgagtactt cccggccatg aaagtggtgc
    13501 aaaagctccc tgcacgcttg acgctccttg gcaattatcc caatcccttc aatgccggga
    13561 cattcatacg gtacgatctt ccggcggcag aggaggtaac ggtgacaatt tacaatttgc
    13621 ggggtgaaaa ggtacgcgag ctttggcacg gggtccagcc ggcgggaagc cacaacctga
    13681 attgggacgg cagcaggatg accggggaag cggtggcctc aggggcttat ctgtgcatca
    13741 tcacagccgg cgaggagatc gcccggctgc gcataacctg ccttaaatga aatgcactgc
    13801 cgtgggaggc gcggatcatg caaattgtca ttcagcccat cgccaatctg agtcagccag
    13861 cataaccctg tggtgtgaaa cgagcgagct caccttcatg attgcgaaca gtcgctatcg
    13921 gtgcgactag tcagccagat gccgggcgct ttacaatctc gacattgggg agctgccaaa
    13981 aggaaatgat acgctccagt tcatcccggg aggccgcccg caggttggga agggtgccgg
    14041 ggcggtcgag gctgttcagt tgcacgcgat ctggggcgat gcgcaggatg gcctccctta
    14101 gcgcagaaag ttcactttcc tgatcggtaa agccgggaac gataaaaacc tccaaccaga
    14161 ttctgccgcc atattcgcgc ctcagctcga ccagcccggc aatgatcttt tctatctgca
    14221 gtatcgggtg gggatgatcg accttacgat agagcgccgg tgaggccgca tcgagggagg
    14281 gcaaaatcag atcggcgcgc agcacctccc tgcgcaccgc cggatcggtg aaaagggtac
    14341 cattggtgag aatggcgagc gggatgtcgg gtgcgcgttc cttgatccat tcaatgagct
    14401 cacccgcgcg gctgtaaagc agcggctctc cggagccgga gaaggtgagg taatccagag
    14461 tggggttggc gatgaaaaag cgggtcaact cttcacacac ctcgtcgaaa gggacgtatt
    14521 cttttcgctc catggtgagg tgggttgtac gcccgcattc acaatagata caattcagat
    14581 tacaggtttt aaggggcacc agatcgatgc ccagagaggt gccgagcctt cgagagtgga
    14641 ccggcccgaa gatatgctgg aacatgtacg tcctacttgc cgggtaaatg gcagcaaaga
    14701 tgttgatggg ataccttacg ttagcgctgt acccagcaga atttccacta ttcgccgggc
    14761 ggccaggccg tcaccatagg gattgacggt tccatgcgaa ctatcaatcc atgatccatt
    14821 cttgagcaca gcagcggcct cagcgacgat cttttcctgc cgggtcccca ccagacgcgc
    14881 aacacccgcc tccacccctt ccggccgttc tgtggtatcg cgcatcacca gcaccggaac
    14941 agcgaagttc ggcgcctctt cctgaatccc ccctgaatcg gtgagaatca ggaccgatcg
    15001 tttcatgagc tgaaccaggg tgaggtagtc gacaggttca ataagatgga cattcccgag
    15061 cccatgcagg atctccccga ccgggcggcg cacattggga ttcagatgca ctggatagac
    15121 aaagtgtgtg tcgtgaaaca aaccggcgag ttcgcgaata gcctgacaga gctcgcgaaa
    15181 tggctcgcca aaactctccc gccggtgggc ggtgatcagg accatgtgac cctcctgcgg
    15241 aatcacatca agggcacttt tccgccaatc gaaagaacgc tgcgagatct ccagcagggc
    15301 atcgacgatg gtgttgccgg tgacgtggat tcccgcttcc ggaaatccct ctgccagcag
    15361 ggcttgccgc gcccgctcag t
//
LOCUS       DAOPWU010000040        15032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4038_length_15032_cov_5.503839, whole genome shotgun sequence.
ACCESSION   DAOPWU010000040 DAOPWU010000000
VERSION     DAOPWU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15032
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4038_length_15032_cov_5.503839"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKM23_03815"
     CDS             <1..652
                     /locus_tag="PKM23_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90634.1"
                     /translation="TPYTTPATTAPITQASPAPGDNPFYKALEQELLKQMPVAFAEFN
                     SQAAAIGEKFPALDQTTRDQLAFHAAQTALKARQQDLNVNHVLQALDTVIKSLSREES
                     EFNTQNEEGFKQKLAGVRQKIGELKQGITARENRLVEIQKELDAFIAARNEEKKRLEH
                     EKSQLLSDQLVTENEINQIEQKKREREADFRSALLAHQQNFTALRERLASNLQKLK"
     gene            752..1786
                     /locus_tag="PKM23_03820"
     CDS             752..1786
                     /locus_tag="PKM23_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90635.1"
                     /translation="MILLIIGLALGFYAISPFLITLLTNAIAVTSKAIVLGGLMALAA
                     LLWWVFSSKRFRSIMYFWRAKFYRWFTGLLIKSGPLDVIYAYVNEYLGEKLETIQSAA
                     DNVYGIRNGIKTNIDNYQSQIKERIKQADALKDRFFEKGAWVDEEKRFQFQKISSEAG
                     MLQSNLEKSQKRFERAEKYCQIMKKMQQAFEFYRDKTKFFADTLAADYNEAQAMAKAT
                     QATSSVLGGDAHSDIYEMSVKYVQGQIALFTGQVDRFMQVAPQFLAEPELNDMVSEDA
                     MMKTLEQWDTASDQMLQQAEDIGKAGQALSTGDPNQAVAIVTAGDKRELVPIMPAKTT
                     GEEEKKKYSW"
     gene            1883..3388
                     /locus_tag="PKM23_03825"
     CDS             1883..3388
                     /locus_tag="PKM23_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HNY90636.1"
                     /translation="MADQAYAGGGSKGGLTLFSKVLIGLLIIGAVGFAAWKFLPEGGS
                     KDPLRVHLITYPGMTPGPYFNNGMSPNPESRFKKEYGLNVKFTVIDVIPDALQAWKTG
                     QIDAAVLTVDSYPIGLEELLTFKPRIFLLTDKSQGADVILASAAVKSVADLAGRKVGY
                     TPGTPSQWFLLNILKAANMTMSDIQGIEFNEVPQVFKAFETGQLDAALGWSPDDITTR
                     ERTPGAHVLKSTKESSEMLFGIMFASQEVIDKRHVDFEHFVEGWLKGAAELNTDQNAR
                     RKAAQIMTAGYSGTNEEYWLETFNNVRLGTYGDNAGFFGLTPGFKGVTAEMIYDEARK
                     AYSAIGFASPNLPSWRNVSDASLVNAARLSGTAHMAQAPATFSPPTSDMYTKPALATK
                     NVRVNFGFNSAVLEPLEALKLKNEYGQIVAMNANSRFRIIGHTDNVGGREYNIELSRR
                     RAKAIVEFFVETYGLSRNQFIYDGKGPDQPIATNSTEEGMALNRRAECSLL"
     gene            3492..4286
                     /locus_tag="PKM23_03830"
     CDS             3492..4286
                     /locus_tag="PKM23_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY90637.1"
                     /translation="MSSPLFELRGELPKSQKIQIGLLGAVVIILLWWLIVKLGLVNPN
                     IVPSPIAIIGSIPELISRDELFKHLGLTVFMNVVSWIEGAAIALPIGFAIGLFPLINA
                     LFGGYVTATRYIPLTATIVLFMAAFGIYMFMKVQFLTFAILVYLITAVVSRVQETPQV
                     YVDAVKTLGANRWQTIKSVFIPHVLSRVFTDFINIVAISWTYAVYVELINKQEGGIGA
                     LIQNLYFRQNEVDKLYMLILIMVGVGFCFDLLLRFIDRKAFPHKYV"
     gene            4298..5092
                     /locus_tag="PKM23_03835"
     CDS             4298..5092
                     /locus_tag="PKM23_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY90638.1"
                     /translation="MTPDFTNTNLADIIELKDISTSYDKGQSYIIKDFNLLIEDIPDK
                     GEFIVILGKSGCGKTTILKYIAGLKEPTSGQVLIRGRERDRSVPISVIFQEPSALEHY
                     TVLKNVMLPLLYKGVPEREAADRAMTMIKAVGLDGHEHKYAKFGKLSGGQVQRVVIAR
                     SLIANPGIILMDEPNRGLDNETALQIDLLIADLWQKLQSTVILVTHNIDQAIFLGSDI
                     YIMGGKPARIVQHFKVPIGFPRTRETKRSREFLDLGGQIEEYLYQL"
     gene            5205..5651
                     /locus_tag="PKM23_03840"
     CDS             5205..5651
                     /locus_tag="PKM23_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNY90639.1"
                     /translation="MMTVRQLLAGKAAGIVTISSQESAFEALQKMAQADIGALIVTEG
                     KRVVGIFSERDYARKVSLRGKTAMACQVGELMTRRVYYAEPAQTINDVMALMTAKRIR
                     HLPVIEKEELVGVISIGDVVKAVIADHEFTIQELEKYITGSYGVEL"
     gene            complement(5658..6791)
                     /locus_tag="PKM23_03845"
     CDS             complement(5658..6791)
                     /locus_tag="PKM23_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90640.1"
                     /translation="MRRYIFTTAAILGLLLGWEVREAAAGTNVLKVRDVFYMTGQTVT
                     VYIDVTNEDPIRDFQFYLRFPAGLTFDGTSFLKTGRILDHSVNCSYQTEGPYVGSFMF
                     LGMNLNGTPFSGNSGAILALTCTVTGGVGSYAVTVPWSKLTGFSGLSSTACSDGMVTI
                     FDPATAARLRVRTRLEGFNITAVMRNELALSGYIPLTSPYAEAPRTASSIPTNVADWI
                     LLELRTTPGGSPVYRQSYLLRADGEVVELNGTTINLGLTLPSGSYWIVLRHRNHAAVM
                     SAAAFALNSTEYRLYDFTLAASQYYGSGGVLSSSRYSMPAGEINQDGRLTSRDYVSWY
                     NKFFTNPGAGYHAEDLNGDALVNNLDFYLWQNNARLGMDSRVP"
     gene            7177..8223
                     /locus_tag="PKM23_03850"
     CDS             7177..8223
                     /locus_tag="PKM23_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNY90641.1"
                     /translation="MRATIADIAQLAHVSKATVSAVLNNRPGISPKTRTRVLELIKKL
                     NYRPNEIARSLSSRKTRTIGLVIKEIDNPYFARIMKGVFDVCRQHAYTVLLGSSELSP
                     AQERQSIEALSSQRVGGLIISPLQGSEVDFTWLADLLRDHYPLVMLGQVKNYHTTVVD
                     IDNVQAARQAVEYLIGLGHRDIAYFAGPSYSSHSDDRLQGYQQALMAHGLPAHPELIL
                     QAGSSRELGYQAGLKLFSEPGIHPTALFCYNDLVAIGLINALADLGIAVPGQVSVIGC
                     DDLEVSRYFRIPLTTIHVPAYEIGRAAAEMVIHDLEEENSANPQIILYEARLVARQSC
                     APALPTLAEEESHE"
     gene            8216..9631
                     /gene="uxaC"
                     /locus_tag="PKM23_03855"
     CDS             8216..9631
                     /gene="uxaC"
                     /locus_tag="PKM23_03855"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNY90642.1"
                     /translation="MNEPLILDENRFFDSDPAIRRIARDLYQGVKDLPIVSPHGHVDP
                     KLFSQNRPFPDPAELILIPDHYIFRMLYSQGIKLESLGIPTIDGTPVATDHRRIWQIF
                     AENYYLFAGTPTGVWLNHELSVIFGIGEKLNGDSAMRIYDMLSEKLLSPEFLPRALFE
                     RMNIEVLTTTDGAADTLAEHQAMRDSGWKGRVLPAFRPDAVTNLANPGWQKAIEELGA
                     ICGFEIDSYARFITALEQRRAFFKQMGALSTDQGVESPWTQELSPVEAEKIFQLARKG
                     QVGKPEADRFTAHMIMEMARMSIEDGLVMQLHPGALRNHNQAIYHRFGPDKGCDIPIQ
                     TEYTRNLQPLLNKYGNDPRLSLVVFTLDETSYARELAPLAGHYPAMKLGPSWWFHDSI
                     EGMTRFRQQVTETAGFYNTTGFVDDTRAFPSIPARHDLARRIDANFLAGLVARHIIDL
                     SDARRVSRALAYELVKQTYKL"
     gene            9647..10891
                     /locus_tag="PKM23_03860"
     CDS             9647..10891
                     /locus_tag="PKM23_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HNY90643.1"
                     /translation="MKLGKYSIGIGDRFGHQGEAQLEALRLLKQEGVAVTPVWNKSYR
                     EHSIINSRPADTRVEADAAVRALGWQEPYFVDADHVGMKTVDHFIAASDFFTIDVADF
                     IGQSAEKEALQEFVDRNLLFAGDLRIPRIPEPLTVALEQIEDIGAKYLFAVEEAGRIY
                     RHIAAEKKGQPFVVEISMDETNAPQTPVELFFILAAIAWKGIPVDTLAPKFTGRFNKG
                     VDYVGDVRQFTREFEEDLAVIAYAVELFKLPAQLKLSVHSGSDKFALYGPINAALKKF
                     NAGLHLKTAGTTWLEEVIGLAEAGGEGLAMARAIYRHAHSRYEELCKPYATVIDIDPA
                     RLPAPDEVDGWEARHYAAVLRHDQSQPLYNPHFRQLIHVGYKVAAEMGERYLDALKRF
                     RPTIARNVTANIYERHLRRIFL"
     gene            11076..11855
                     /locus_tag="PKM23_03865"
     CDS             11076..11855
                     /locus_tag="PKM23_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNY90644.1"
                     /translation="MSYVEELFALKGKTAVAIGGGGVLAGKMAEGLARAGADIAILDL
                     NPDNAQKQADECRKAGVKTLAVKVDASSKEELRAAADTILAKLGRVDILINAPGINSS
                     TPFLDIEEEEYDKIMRVNLKSIFLACQIFGKIMIDQGEGGSIINISSASSAPPLSKVF
                     TYSITKAGVNNLTQNLAREWAPFKVRVNALAPGFFPAEQNRKILTPERTADIMRHTPM
                     ARFGDAEELVGAVIWMSSPKAASFVTGAIIRVDGGFTAMTI"
     gene            11877..13289
                     /gene="gnd"
                     /locus_tag="PKM23_03870"
     CDS             11877..13289
                     /gene="gnd"
                     /locus_tag="PKM23_03870"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNY90645.1"
                     /translation="MNSYSIGVIGLGVMGRNLALNIERNGYPVAGFDLDEKQRTSAAE
                     KFTGKKMTVCPTLTEFVSLLESPKKILMMVPAGKPVDAVIQELIPHLAPGDILIDGGN
                     TYFPDTERRTRELTAKGFHYIGTGVSGGEEGALWGPSIMPGGAPAAWPHVKPILQAIA
                     AKVADGTPCCEWVGNGGAGHFVKMVHNGIEYGDMQMICEAYYLMERVLGLSAAQMGEV
                     FHTWNQGDLNSYLIEITAAIMTKKDERTGRPMIDVILDTAGQKGTGKWTSQVALDLGV
                     PAMTIAEAVFARTLSAIKEERVAAEKVLPGPEVHFNGDKTAFIEQIRQALYAAKICSY
                     AQGFQLMAAASREFNYGLNFGQIALLWRGGCIIRAQFLDKIKKAFDDQPDLPNLLLAP
                     YFRTAMASHHIAWRAVVSKAVELGISIPAFSSALAYYDGYRSGRLPANLLQAQRDYFG
                     AHTYERIDAPRGQFFHTEWL"
     gene            13357..14439
                     /locus_tag="PKM23_03875"
     CDS             13357..14439
                     /locus_tag="PKM23_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNY90646.1"
                     /translation="MQKLDIRQDACDLDFLSVGALVHRLDPGVIPFRKARSFNVHVSG
                     GEYNVAANLADCFGLTTGIATAMVKYPIGEMIQARVREMGVRPYYKWFDHDGVRGPNM
                     ATVYSDLGMGVRPPVVFYNRSNEAGALLKPGDIDWKSIFAGGVRWFHSGGIFAALSET
                     TSELILEGMRTARAHGAINSFDLNYRAKLWAPIGGLKKAQQVLRSIVSEIDVLVGNEE
                     DMQLGLGIPGPETTDRSGIKSDAFFAMIEKVVKEFPNIKAVATTLREVQSSNRHDWGA
                     VLWLKGDRYLSPSCTLDVLDRIGGGDGFASGLIYGLINGRVPEEALCLGWAHGALLTT
                     FPGDVTMARLPEVEAFAKGGSARVQR"
     gene            14710..14910
                     /locus_tag="PKM23_03880"
     CDS             14710..14910
                     /locus_tag="PKM23_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNY90647.1"
                     /translation="MEKGTVKWFNASKGYGFITREQGGDVFVHFNAIQGTGYKSLDEG
                     DQVEFEVTQGQKGLQASNVTKL"
BASE COUNT         3237 a         5072 c         3958 g         2765 t
ORIGIN      
        1 aacgccctac accacaccag ccacgaccgc gcccatcact caggccagcc cggctcctgg
       61 ggataacccc ttttacaagg ccctcgagca ggaactgctg aaacagatgc ccgtcgcttt
      121 cgccgagttc aacagccagg cggcggccat cggcgaaaaa ttccctgccc tcgaccagac
      181 cacccgcgac cagcttgcct tccacgccgc ccagaccgcc ctcaaggcgc gccagcagga
      241 tctgaacgtc aaccatgtgc tgcaggccct cgacacggtg atcaagtccc tctcccgcga
      301 agagtcagaa ttcaacaccc agaacgaaga gggctttaaa cagaaactgg ccggcgtacg
      361 ccagaagatc ggcgaactga agcagggcat caccgcccgc gaaaaccgtc tggtcgagat
      421 ccagaaggaa ctcgacgcct tcattgccgc ccgcaatgaa gaaaagaaga ggctggaaca
      481 tgaaaaatcc cagctcctct ccgatcaact cgtgacagaa aacgaaatca atcaaatcga
      541 gcagaagaaa cgcgaacgag aagccgattt tcgcagtgcc ctgctggccc atcagcagaa
      601 tttcaccgcc ctgcgcgaac ggctggcgtc caatctgcaa aaactgaaat agcaagcgga
      661 ggattccatg agcgagatgc cctcactgcc gggcgctgac aaagtatcag ccttcgtatc
      721 caaaaaagag aaaactgtgg gaagcttcgg catgatcctg ctgatcatcg gcctggccct
      781 cggcttttat gccatctccc cctttctcat caccctgttg accaacgcca tcgccgtcac
      841 cagcaaggcc atcgtcctcg gcggcctcat ggccctcgcc gccctgctct ggtgggtctt
      901 ttccagcaag cgcttccgtt ccatcatgta tttctggcgc gccaagttct accgctggtt
      961 caccggcctg cttatcaaga gcggacccct cgacgtcatc tatgcttatg tcaatgaata
     1021 cctgggcgaa aaactcgaaa ccatccagag tgccgctgat aatgtctacg gcatccgcaa
     1081 cgggatcaag accaatatcg ataactatca gagccagatc aaggagcgca tcaaacaggc
     1141 ggacgccctc aaggaccgct ttttcgagaa gggcgcctgg gtcgatgagg agaaacgctt
     1201 ccagtttcag aagatcagca gtgaagccgg catgctccag tcgaacctcg aaaaatcgca
     1261 gaagcgcttc gaacgcgccg agaagtattg ccagattatg aaaaaaatgc agcaggcctt
     1321 cgagttctac cgcgacaaga ccaagttctt cgccgatacc ctggccgccg attataacga
     1381 agcccaggcc atggccaaag ccacccaggc cacctccagc gtcctcggcg gcgacgctca
     1441 cagcgatatc tacgaaatgt cggtcaagta cgttcagggc caaatcgccc tcttcaccgg
     1501 acaggtcgac cgcttcatgc aggtcgctcc ccagttcctc gccgaaccgg agctcaacga
     1561 catggtcagc gaggatgcca tgatgaaaac ccttgagcag tgggacaccg cgagcgacca
     1621 gatgctccag caggctgagg atatcggcaa agccggccag gcgctttcaa ccggcgatcc
     1681 caaccaggca gtggccatcg tcaccgccgg cgacaagcgc gaactggtgc cgatcatgcc
     1741 ggccaaaacc acgggcgaag aagagaagaa aaagtacagc tggtaaggaa agctccaccg
     1801 ccgcaccggc gagcgaacgc tcttgctcgc aaaggggccg gcgcagcggg agccatcgat
     1861 caacccaatc aaggagcaag atatggcaga tcaagcgtac gcaggcggag gcagcaaggg
     1921 aggcctcacc cttttttcaa aggttctcat cggcctgctc atcatcggcg ccgttggctt
     1981 cgccgcctgg aagtttttgc ccgaaggcgg cagcaaggat cccctgcgcg tccacctcat
     2041 cacctatcct ggcatgacgc cgggccccta tttcaataac ggcatgagcc ccaatcccga
     2101 atcgcgcttt aaaaaggagt acgggctcaa cgtcaagttc accgtcatcg acgtcatccc
     2161 cgatgccctc caggcctgga agaccggcca gattgatgcg gctgtcctca ccgtcgattc
     2221 ctatcccatc ggcctcgagg aactgctcac cttcaagccg cgcatctttt tgctcacgga
     2281 caaatcccag ggcgccgacg tcattctcgc ctccgccgcg gtcaaatcgg tcgccgatct
     2341 cgccggccgc aaggtgggct acacccctgg cacgccgtcg cagtggttcc tgctcaacat
     2401 cctcaaagcg gccaacatga ccatgagcga catccaggga atcgaattca acgaggtgcc
     2461 ccaggtcttc aaggccttcg agaccggcca gctcgacgcc gcgctcggct ggtcaccgga
     2521 cgatatcacc acccgcgaac gcacgcccgg cgcccatgtc ctcaagagca ccaaggaatc
     2581 ctccgaaatg ctcttcggca tcatgttcgc cagccaggag gtgatcgaca agcgccacgt
     2641 cgactttgaa cactttgtcg aaggctggtt gaagggggcc gccgaactca acaccgacca
     2701 gaacgcccgc cgcaaggcag cccaaatcat gaccgcaggc tacagcggca ccaacgagga
     2761 gtactggctc gaaactttca acaatgtccg cctgggcacc tacggcgaca acgccggatt
     2821 tttcggactc acccccggct tcaagggggt gactgcggag atgatctacg acgaggcccg
     2881 caaggcctat tcggccatcg gcttcgcctc ccccaacctg ccgagctggc gcaacgtctc
     2941 ggatgccagc ctcgtcaacg ccgcccgcct cagcggcacg gcccatatgg cccaggcccc
     3001 ggccaccttt tctccgccga cctcggatat gtacaccaaa cccgccttgg ccaccaagaa
     3061 cgtgcgcgtc aactttggtt tcaactcggc cgtgctcgag cccctcgagg ccctcaagct
     3121 caagaatgaa tacggccaga ttgtcgccat gaatgccaat tcgcgcttcc gcatcatcgg
     3181 ccacaccgac aacgtcggcg gccgcgaata caacatcgaa ctctcgcgcc gccgcgccaa
     3241 ggccatcgtc gagttcttcg tcgagaccta tggcctgagc cgcaaccagt tcatttatga
     3301 cggcaagggc cccgaccagc ccatcgccac caactcaacc gaggagggca tggcgctcaa
     3361 ccgccgcgcc gagtgctctc tgctttaatc ccttgttcga catgccggct cgtcatgagc
     3421 ggcgggccgg catggtttca ctggcgccct cttccatacc ggcgcctttc catatccact
     3481 ccggaggcat catgtcttcc ccccttttcg aactgcgcgg tgagctgccc aagagccaga
     3541 agatccagat cggcctgctt ggcgccgtgg tcatcatcct gctctggtgg ctgatcgtca
     3601 aactgggcct cgtcaacccc aacatcgtcc cctcccccat cgccatcatc ggctccatcc
     3661 ccgagctgat cagccgggac gaactcttca aacacctcgg tctgaccgtt tttatgaacg
     3721 tcgtcagctg gatcgaaggc gcggcgatcg ccctgcccat cggctttgcc atcggcctct
     3781 ttcccctcat caacgccctc ttcgggggct atgtcaccgc cacgcgctat atccccctca
     3841 ccgccaccat cgtcctcttc atggcggcgt tcggcatcta tatgttcatg aaagtccagt
     3901 tcctcacctt tgccatcctc gtctacctca tcaccgccgt cgtctcccgc gtacaggaga
     3961 ccccgcaggt ctatgtcgac gcggtcaaaa ccctgggggc gaaccgctgg cagacgatca
     4021 aaagcgtctt catcccgcat gtcctttcgc gggtctttac cgatttcatc aacatcgtcg
     4081 ccatcagctg gacctacgcc gtctatgtcg aactgatcaa caaacaggag ggcggcatcg
     4141 gcgccctcat ccagaacctc tatttccgcc agaacgaagt ggacaagctc tatatgctca
     4201 tcctgatcat ggtcggtgtc ggcttttgtt tcgatctgct gctgcgtttc atcgaccgca
     4261 aggcatttcc acataagtac gtataaggcg atcccgtatg actccagact ttaccaatac
     4321 caatctggcc gatatcatcg aattgaagga tatcagcacc agctacgaca agggccagag
     4381 ctatatcatc aaggatttca acctgctcat cgaggacatc cctgacaagg gcgagttcat
     4441 cgtcatcctc ggcaaatcgg gatgcggcaa gaccaccatc ctcaaataca ttgcgggatt
     4501 gaaggagccc acctccggcc aggtcctcat ccgcgggcgc gagcgcgacc gctcggtccc
     4561 catcagcgtc atcttccagg agccctccgc cctcgagcac tataccgtgc tcaagaacgt
     4621 catgctcccc ctgctctaca agggggtgcc tgagcgcgag gccgccgacc gggccatgac
     4681 catgatcaag gcggtcgggc tggacggcca cgagcacaaa tacgccaagt tcggcaagct
     4741 ctcgggtgga caggtgcagc gcgtggtcat cgcccgcagc ctcatcgcca atcccggcat
     4801 catcctcatg gatgagccca accgcgggct cgacaacgag accgccctgc agatcgacct
     4861 gctcatcgcc gacctttggc agaaactcca gtccacggtc atcctcgtca cccacaacat
     4921 cgaccaggcc atttttctcg gcagcgatat ctacatcatg gggggcaagc cggccaggat
     4981 cgtccagcac ttcaaggtgc cgatcggctt tccgcgcacc cgcgagacca agcgcagccg
     5041 ggagtttctc gacctgggcg gccaaatcga ggagtatctc taccagctct gagccgcaaa
     5101 tcgccgcagt gcgcaaaata gagtccgttt caatcccgaa ggcaggaatc ccgattcctg
     5161 cctttgtggt tatagcggaa aagggattcg ctcaaggagg gtatatgatg actgtaagac
     5221 agctgctggc gggcaaagcc gcggggattg tgaccatttc atcgcaggag agcgcctttg
     5281 aagccctgca aaagatggcc caggccgata tcggtgccct gatcgtgacc gagggcaaac
     5341 gggtggtggg gattttttcg gaacgggact atgcgcgcaa ggtgagcctg cgcggcaaga
     5401 cggccatggc ctgccaggtc ggcgagctga tgacgcggcg ggtctactac gccgagcccg
     5461 cccagaccat caacgacgtc atggccctga tgacggccaa acgcatccgc catctgcccg
     5521 tcattgaaaa ggaagaactg gtgggggtaa tttcgatcgg ggatgtggtc aaggcggtca
     5581 tcgccgacca cgaattcacc atccaggagc tggaaaaata tatcaccggc agctacgggg
     5641 tggaactata aatccgctca cggcacccgg gagtccatcc ccaaacgcgc gttgttctgc
     5701 cagaggtaaa aatcaaggtt attaaccagg gcgtccccgt tcagatcctc ggcatgatag
     5761 ccggcgccgg gatttgtaaa aaacttgttg taccaggaga cataatcccg gctggtgaga
     5821 cggccgtcct ggttgatctc accggccggc atgctgtagc ggctggagct caggactcct
     5881 ccactgccat aatactggct tgccgcaagg gtgaaatcgt agaggcggta ttcggtcgag
     5941 ttgagggcaa aggcggctgc gctcatcacc gcggcgtggt tgcggtgacg cagcacgatc
     6001 cagtagctcc cggagggcag ggtgaggccg agattaatgg tagtgccgtt gagctcgacc
     6061 acctccccgt ccgccctgag cagataggac tgccggtaga ccggcgatcc ccccggtgtc
     6121 gtgcgcagct cgagcaggat ccagtcggcc acattggtgg ggatggagga ggccgtgcgc
     6181 ggggcctcgg cgtagggcga ggtcaggggg atgtagccgc tcaaggccag ctcgttgcgc
     6241 atgaccgcgg tgatattaaa gccctccagc cgggtacgga cccgcagccg ggccgccgtg
     6301 gcgggatcga agatggtgac catgccgtcc gagcaggctg ttgagctgag gccggaaaac
     6361 ccggtcagct tggaccaggg caccgtcaca gcatagctcc ccactccgcc cgtgaccgta
     6421 caggtcaggg caagaatggc gccgctgttg ccggagaagg gagtcccgtt cagattcatg
     6481 ccaagaaaca taaaagatcc tacataagga ccttcggttt gatatgaaca gttaaccgag
     6541 tgatcaagaa tccgtccggt tttgagaaag gaagtgccgt caaatgtcaa tccggcagga
     6601 aatctcaaat agaattgaaa gtccctgatc ggatcctcat tggtcacatc gatatagacc
     6661 gtaacggtct ggccggtcat ataaaaaaca tcccgcacct tcaatacatt ggtgcctgca
     6721 gccgcctccc gcacctccca gccgaggagc aaacccagga tcgccgccgt tgtgaaaata
     6781 taccgccgca tatgattcat ttcattccct gccataaaaa ggtcatacct tcgttcaacc
     6841 cggcatcggg ccgggtagca cgtccctatt cttctcctta atataataca gaaaattcaa
     6901 tgaattggag aaaattatcc ctgccggtat gagcggcgtc acaatttgcc gtttcttatc
     6961 cccgtgccgc gccggcgcag cctcgttgcc tcataataaa tgtaaccgaa atacttttct
     7021 ttttcccatc cggtgatggc gcagccggcg gtttttttaa ataatccttg acattggcgg
     7081 caaatctgct aaattgaaga cactgaaccg gttcagatat attagcacag aaaattaatt
     7141 taatttgatt tatggaatag ataatcggtt tacatcatgc gcgctaccat cgccgatatc
     7201 gcccagctcg cccacgtctc caaggccacg gtctcggccg tcctcaacaa ccggccgggc
     7261 atctccccca aaacccgcac ccgtgtcctc gaactcatca aaaaactcaa ctaccgccct
     7321 aacgaaatcg cccgctccct ctccagccgt aaaacccgca ccatcggact ggtcatcaag
     7381 gagatcgaca acccctactt cgcccgcatc atgaagggcg tcttcgacgt ctgccgccaa
     7441 cacgcctata ccgtcctgct tggcagctcc gaactctccc ccgcccagga acgccagagc
     7501 atcgaggccc tctccagcca gcgcgtcggc ggcctcatca tctcccccct ccagggcagc
     7561 gaggtcgatt ttacctggct cgccgacctg ctccgcgacc actatcccct ggtcatgctc
     7621 ggccaggtca aaaactacca caccaccgtg gtcgacatcg acaacgtcca ggcagcccgc
     7681 caggccgtcg aatacctcat cgggctcggc caccgcgata tcgcctactt cgccggcccc
     7741 tcctactcca gccatagcga cgaccgcctg cagggctacc agcaggccct catggctcat
     7801 ggactgccgg cccaccctga gctcatcctc caggccggct ccagccgtga actcggctat
     7861 caggcgggcc tcaagctctt ttccgagccc ggcatccatc caaccgcgct cttttgctac
     7921 aacgacctcg tcgccatcgg cctcatcaac gccctcgccg atctcggcat cgccgtcccc
     7981 ggccaggtct cggtcatcgg ctgcgacgac ctcgaggtct cccgctactt tcgcattccc
     8041 ctcaccacta tccacgtccc ggcctacgag atcggccggg cggccgccga gatggtcatc
     8101 catgatctcg aagaggaaaa cagcgccaac ccgcagatca ttctctatga agcaaggctg
     8161 gtcgcacgtc aatcctgcgc accggcccta cccaccctag ctgaggaaga gtcccatgaa
     8221 tgaacccctg attctggatg aaaaccgctt tttcgattcc gatccggcga tccggcgcat
     8281 cgcgcgcgac ctctaccagg gggtcaagga tctgccgatc gtcagccccc acgggcatgt
     8341 cgaccccaaa ctcttctccc agaaccgccc ctttcccgat ccggcggagc tgatcctcat
     8401 ccccgaccac tacatcttcc gcatgctcta ctcccagggc ataaagctgg aaagcctcgg
     8461 catccccacc atcgacggca ccccggtggc cacggaccac cgccgcatct ggcagatctt
     8521 tgcggaaaac tattatctct tcgccggcac cccgaccggg gtctggctca accacgagct
     8581 ctccgtcatt ttcggcatcg gagaaaagct caacggcgac tccgccatgc gcatctacga
     8641 catgctgtca gaaaaactgc tctcgccgga gttcctgccg cgcgccctct tcgaacgcat
     8701 gaatatcgag gtgctcacca ccaccgacgg cgctgccgac accctcgccg agcatcaggc
     8761 catgcgcgat tcaggctgga aaggccgcgt ccttcccgcc ttccgccccg acgcggtcac
     8821 caacctcgcc aatcccggct ggcagaaggc catcgaggag ctcggggcga tctgcggctt
     8881 tgagatcgac tcctacgccc gtttcatcac cgccctcgaa cagcgccgcg ccttcttcaa
     8941 gcagatgggc gccctctcca ccgaccaggg ggtcgaatcg ccctggaccc aggaactctc
     9001 cccggtcgag gctgaaaaga tcttccagct cgcccgcaag ggccaggtcg gcaagcctga
     9061 ggccgaccgc ttcaccgccc acatgatcat ggagatggcc cgcatgagca tcgaggacgg
     9121 cctggtcatg cagctccacc ccggcgccct gcgcaatcac aaccaggcca tctaccaccg
     9181 cttcggacct gacaagggct gcgacatccc gatccagacc gagtacaccc gcaacctcca
     9241 gcccctgctc aacaagtacg gcaacgaccc ccgtctgagc ctggtcgtct tcaccctcga
     9301 cgagaccagc tatgcgcgcg agctcgcccc cctggccggc cactatccgg cgatgaagct
     9361 ggggccctcc tggtggttcc acgacagcat cgagggcatg acccgcttcc gccagcaagt
     9421 gaccgagacc gccggctttt acaacaccac cggctttgtc gacgacaccc gcgccttccc
     9481 ctcgatcccg gcccgccacg atctcgcccg gcgcatcgac gccaatttcc tcgccggcct
     9541 cgtcgcccgc cacatcatcg acctgagcga cgcgcgccgc gtcagccgcg ccctcgccta
     9601 cgaactggtc aaacaaacct ataaacttta agcccgggag gcccccatga aacttggaaa
     9661 atattcgatc ggcatcggcg accgcttcgg tcatcagggt gaagcccaac tcgaggccct
     9721 ccgcctgctc aaacaggagg gcgttgcggt cactcccgtc tggaacaaat cctatcgcga
     9781 acactcgatc atcaacagtc ggccggccga cacccgcgtc gaggccgacg ccgctgtccg
     9841 ggccctgggc tggcaggaac cctatttcgt cgatgccgac catgtcggca tgaaaactgt
     9901 ggaccatttt atcgccgcca gcgatttttt caccatcgat gtcgccgact ttatcggcca
     9961 gagcgccgag aaagaggctc tccaggaatt tgtcgaccgc aatctgcttt tcgctgggga
    10021 tctgcgcatc ccccggatcc ctgaacccct tacggtcgcc ctcgaacaga ttgaggatat
    10081 cggtgccaaa tatctcttcg cggtcgagga ggccgggcgc atctatcgcc acatcgccgc
    10141 agagaaaaag ggccagccct tcgtcgtcga aatctcgatg gatgagacta acgccccgca
    10201 gactccggtc gagctcttct tcatcctcgc cgccatcgcc tggaagggaa ttccggttga
    10261 tacccttgcc cccaaattca ccggccgctt caacaagggg gtcgattacg ttggcgacgt
    10321 ccgccagttc acccgcgagt tcgaggagga cctggcggtg atcgcctacg ccgtcgagct
    10381 cttcaagctg cccgcgcagc tcaaactgag cgtccactcc ggcagcgaca aattcgccct
    10441 ctatggcccg atcaacgccg ccctaaaaaa attcaacgcc gggctccatc tcaagactgc
    10501 cggcaccacc tggctcgagg aggtcatcgg actggccgag gccgggggag agggactggc
    10561 catggccagg gcgatctacc gtcacgccca ttcccgctac gaggagctct gcaagcccta
    10621 tgcaacggtg atcgacatcg atccggcacg gctgcccgcg cctgacgagg ttgacggttg
    10681 ggaggcccgc cactacgccg ccgtgctgcg ccacgaccag agccagccgc tctacaaccc
    10741 ccacttccgt cagctgatcc acgtcggcta caaggtggcc gcggagatgg gcgagcgcta
    10801 cctggatgcc cttaaaaggt tccgtccgac catcgccaga aatgtcaccg ccaatatcta
    10861 cgagcgccat ctgcgccgga tttttctgtg aggtcttgaa taactcgcgc caagacgcgg
    10921 agaggcagag aaaaaatatc tctcacggaa gcacaagaca caaagaatag ctatcttttt
    10981 atttctttgt ggcttggcgg cttggtgaga gtattgtttt ctctgcgctc tctgcgcgaa
    11041 aatatatatt atcaaaaaac caaaaggagt acatcatgag ctatgttgaa gaactcttcg
    11101 ctttgaaagg caagaccgct gtggccatcg gcgggggggg cgtcctcgcc ggcaagatgg
    11161 ctgaaggcct cgcccgcgcc ggagccgata tcgcgatcct cgacctcaat cccgataatg
    11221 cgcaaaaaca ggccgatgag tgcaggaagg ccggagtcaa aaccctggcg gtcaaggtcg
    11281 acgcctccag caaggaggag ctgcgcgcag ccgcagacac cattctcgcc aagctcgggc
    11341 gggttgatat cctcatcaac gccccgggca ttaactcttc cacccccttt ctcgacatcg
    11401 aggaggagga gtacgataaa atcatgcgcg tcaacctcaa gagcatcttc ctcgcctgcc
    11461 agatcttcgg caagatcatg atcgatcagg gcgagggcgg cagcatcatc aacatctcct
    11521 ccgcctcctc tgcgcctccc ctctccaagg tcttcaccta cagcatcacc aaggccggag
    11581 tcaacaacct gacccagaat ctggcccgcg aatgggctcc cttcaaagtg cgcgtcaatg
    11641 ccctcgcccc gggctttttc ccggccgagc agaaccgcaa gatcctcacc cccgaacgca
    11701 ccgccgatat catgcgtcat acccccatgg cccgcttcgg agatgccgag gagctggtcg
    11761 gcgccgtgat ctggatgtcc tctcccaagg cggcctcctt cgtcaccggg gccatcatcc
    11821 gcgtcgacgg cggctttacc gccatgacca tctaaccgag ccggaggaga acgaagatga
    11881 attcctattc catcggggtt atcggcctcg gggtcatggg ccgcaacctg gctctcaata
    11941 tcgaacgcaa cggctacccg gtcgccggtt ttgacctcga cgaaaagcag cgcacctccg
    12001 cagcggaaaa attcaccggc aaaaagatga ccgtctgccc caccctcacc gaatttgtca
    12061 gcctcctgga gagccccaag aaaatcctca tgatggtccc ggccggcaag ccggtcgatg
    12121 ccgtcatcca ggagctgatc ccccatctag ccccgggcga catcctcatc gacggcggca
    12181 acacctactt ccccgacacc gagcgccgca cccgagagct cactgccaaa ggcttccatt
    12241 acatcggcac cggggtgagc ggcggcgagg agggcgccct ctggggcccc tcgatcatgc
    12301 ctggcggcgc gccggccgcc tggccccacg tcaagcccat cctgcaggcc atcgccgcca
    12361 aagtggccga cggcaccccc tgctgcgaat gggtcggcaa cggcggggcc ggccatttcg
    12421 tcaagatggt ccataacggc atcgaatatg gggatatgca gatgatctgc gaggcctatt
    12481 acctgatgga acgggtgctc ggcctctccg ccgcccaaat gggcgaggtc tttcacacct
    12541 ggaaccaggg tgacctcaac agctatctta tcgagatcac cgccgccatc atgaccaaaa
    12601 aggacgagcg caccggccgg ccgatgatcg atgtcatcct cgacaccgcc ggccagaagg
    12661 ggaccggcaa atggaccagc caggtcgccc tcgatctcgg cgtcccggcg atgaccatcg
    12721 ccgaggcggt tttcgcccgc accctcagcg ccatcaagga ggagcgcgtc gccgccgaaa
    12781 aagtgctgcc gggaccggag gtgcacttta acggcgacaa gacggccttc atcgaacaga
    12841 tccgccaggc cctctatgcg gccaagatct gctcctatgc ccagggcttc cagcttatgg
    12901 ctgccgcctc cagggaattc aactacggcc tcaatttcgg ccagatcgcc ttgctctggc
    12961 gcggcggctg catcatccgc gcccagttcc tcgacaagat caaaaaggcc ttcgatgacc
    13021 agcccgacct ccccaatctc ctcctcgcgc cctacttccg cacggccatg gccagccatc
    13081 acatcgcctg gcgcgccgtg gtgagcaagg cagtcgaact gggcatctcc atccccgcct
    13141 tctccagcgc cctggcctat tatgacggct accgcagcgg ccgcctgccc gccaatctgc
    13201 tccaggccca gcgcgactat ttcggcgccc atacctacga acgcatcgat gccccaaggg
    13261 gccagttctt tcacaccgaa tggctgtaga aaatcaggac gtccgggcgg gctggccccg
    13321 cccggacgca ctgcttattg agacaaagga gacaccatgc aaaaactcga tatccgtcaa
    13381 gacgcctgcg acctcgattt cctctcggtc ggcgcattgg tccaccgcct cgaccccggg
    13441 gtaatcccct tccgcaaggc gcgcagcttc aacgtacatg tctcgggcgg cgaatacaat
    13501 gtcgccgcca atctcgccga ctgcttcggc ctcaccaccg gcatcgccac cgccatggtc
    13561 aaatacccca tcggcgagat gatccaggcc cgcgtgcgcg agatgggggt ccgaccctat
    13621 tacaaatggt ttgaccacga tggagtgcgc ggccccaaca tggccaccgt ctacagcgac
    13681 ctcggcatgg gggtgcgccc gccagtggtt ttttacaatc gctccaacga ggctggcgcc
    13741 ctgctcaagc cgggcgacat cgactggaag agcatcttcg ccggcggcgt gcgctggttc
    13801 cactccggcg gcatcttcgc cgccctctcc gaaaccacct cggagctgat cctcgagggc
    13861 atgcgcaccg cccgcgccca cggcgcgatc aactccttcg acctcaacta ccgggccaag
    13921 ctctgggccc ccatcggggg actcaagaaa gcccagcagg tgctgcgcag catcgtctcc
    13981 gaaatcgatg tcctggtcgg caacgaagag gatatgcagc tcggcctcgg catccccggt
    14041 ccggagacga cggacagatc cgggatcaag agcgatgcct ttttcgccat gatcgaaaag
    14101 gtggtcaagg agttccccaa tatcaaggcc gtggccacca ccctgcgcga ggtgcagtcc
    14161 agcaaccgcc acgactgggg cgccgtgctc tggctcaagg gcgaccgcta cctctccccc
    14221 agctgcaccc tcgatgtgct cgaccgcatc ggcggcggcg acggcttcgc ctctgggctg
    14281 atttacggcc tcatcaacgg ccgcgttcct gaggaggccc tgtgccttgg ctgggcccac
    14341 ggcgccctgc tcaccacctt tccgggcgac gtcaccatgg cgcggctgcc ggaggtggaa
    14401 gccttcgcca agggcggctc agcccgcgtt cagcgctgag gccgcgacgc cccccctggg
    14461 ttcccggccc cccttcccgg ggccgggcgc ctgccggagg cgctgccgca gacacgctat
    14521 gtaaaaacag tatttagcaa tatttctctt gcattttatt gaaataaatt gtaatatgac
    14581 tgataattaa agtacacttt ttcatccgcc tttaggtgtt tggttgtcaa cctctgcaag
    14641 aggtcaaccc gtggcaagac gataaaagtg gtactaggtt gtagttacct atttgcaggg
    14701 gtttcaaaga tggaaaaagg aactgtcaag tggttcaacg cctccaaagg ctacggtttc
    14761 atcaccagag aacagggcgg cgacgtgttt gtgcatttca atgccattca gggcaccggt
    14821 tacaaatccc tggatgaggg tgatcaggtc gagttcgaag tcacccaggg ccagaaaggc
    14881 ctccaggcca gcaacgtcac caagctctaa gcttgacgcg gacgaacttg aaccccggtc
    14941 ccaacggacc ggggtttttt atttctgtcc ccgcaccggc cctcctccgc aatgaacaaa
    15001 cttttgcttg caaaataggc ggtttctgat ta
//
LOCUS       DAOPWU010000041        15027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4043_length_15027_cov_5.321200, whole genome shotgun sequence.
ACCESSION   DAOPWU010000041 DAOPWU010000000
VERSION     DAOPWU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4043_length_15027_cov_5.321200"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="PKM23_03885"
     CDS             complement(<1..1086)
                     /locus_tag="PKM23_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNY90648.1"
                     /translation="MRIEVISIGDELLIGQTINTNAAWIGQELLEAGMEVGRVTTVGD
                     GLGQIKQALAEAAGRADYILVTGGLGPTNDDITRTAACEYFGVGLIRDEATLDHIRRL
                     FKRRNLPMASVNESQALVPETARLMQNDRGTAPGYIFERDGRHYYFMPGVPYEMKAMM
                     TAVVLPELQKAKGGQVILTHHIATIGIAESSLYEQIGGIPEIEALVRLAFLPSPSGVR
                     IRLNAMGEHEEEVRARMARAVERVCCNIRPYIFAERNIPLEQALAEELISRQEHLGVA
                     ESCTGGLIANKFTNLAGSSRFFERGLVTYSNEAKIQFLGVPEDLIALHGAVSAPVAEA
                     MAEGVVRVSGTDHGLSVTGIAGPDGGTP"
     gene            complement(1106..1579)
                     /locus_tag="PKM23_03890"
     CDS             complement(1106..1579)
                     /locus_tag="PKM23_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNY90649.1"
                     /translation="MNEKRQWRDEVARFLATTLYTGYFPVAPGTVGAAAAVLLLWFVG
                     MDQGFLLLGLVLLTTLIGVWAAGRAEALWQETDPGRVNLDEVAGMMVSLLFLPRTGVV
                     FILAFVAFRVFDILKPVPVSTAERLPHGWGIMADDIVAGIYANIVLQLVVYFRLF"
     gene            complement(1708..2304)
                     /gene="recR"
                     /locus_tag="PKM23_03895"
     CDS             complement(1708..2304)
                     /gene="recR"
                     /locus_tag="PKM23_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNY90650.1"
                     /translation="MNYSSEAVERAIHELSKLPGIGRKSAQRLVFFLLKVPEEEVRAL
                     AGSLAELHEKVRYCSRCFNITEEDPCPICADEMRDRRLICVVEEANDVVALEKTGEFK
                     GLYHVLGGALSPLDGIGPDDLKIKELLGRLSEEVEEIILATNPNTEGEATALYLARLF
                     KPMQVRVSRIARGIPVGADIEYADEMTLSRAMAGRVPL"
     gene            complement(2333..2677)
                     /locus_tag="PKM23_03900"
     CDS             complement(2333..2677)
                     /locus_tag="PKM23_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNY90651.1"
                     /translation="MFNKGNMGGLLRQAQKMQEDLQKMQANLANLRVEGTAGGNMVTV
                     TANAAQEILQVKINPEVVDPEDVEMLEDLVLAAVNQALQNGRAKAEEEMAKVTGGMDP
                     GGMLGGLKLPGM"
     gene            complement(2694..4400)
                     /gene="dnaX"
                     /locus_tag="PKM23_03905"
     CDS             complement(2694..4400)
                     /gene="dnaX"
                     /locus_tag="PKM23_03905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNY90652.1"
                     /translation="MSYLVLARKYRPMFFADVLGQQHVTHTLQNAIQQNRIANAYLFS
                     GPRGIGKTTVARLLAKALNCDQGPTINPCNTCTSCIEINESRSLDVFEIDGASNRGID
                     EVRNLREGLRYAPHPGKYRIYIIDEVHMLTKEAFNALLKTLEEPPARVLFIFATTESH
                     KVPATILSRCQRFDFKRMSHQFIVEQLQTMCRQEEIAIDAESLRMIALKADGSMRDSQ
                     SILDQIIAFAGKNIQARDVASLLGIIDQELFFRVTDLIQAGDVDGAIKLADHLFAEGF
                     DFDEFLIGLAEHLRNFLVVKSGSDPHLIDCSEEHIVRYREQIEAFSIEDLLRLIKVAA
                     DAENLVRRSSNARLHFEVGLVKMVRMSSSVQLTTLMEQLGEVKKKSNSLPLSSSTSPG
                     YKPSFLAQTPNPTTSRPSAAPSASAPAGPALIPADAVSPEQKAVVEQAPALSLAAVEE
                     RWQGVLDAVRTQKVALGTFLQEGWPVKVGAGQLEIMFGRESSFQMNAVENNRLLLQEI
                     MTGILGVPLRLLCVKDEEGILPRVRKIPALTDRKSEFEKKLRDDVRMQRIVQEFDAEF
                     IK"
     gene            complement(4435..4702)
                     /gene="ffs"
                     /locus_tag="PKM23_03910"
     ncRNA           complement(4435..4702)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKM23_03910"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4712..4784)
                     /locus_tag="PKM23_03915"
     tRNA            complement(4712..4784)
                     /locus_tag="PKM23_03915"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4749..4751),aa:Ala,seq:cgc)
     gene            complement(4841..4929)
                     /locus_tag="PKM23_03920"
     tRNA            complement(4841..4929)
                     /locus_tag="PKM23_03920"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4893..4895),aa:Ser,seq:gga)
     gene            complement(4955..5041)
                     /locus_tag="PKM23_03925"
     tRNA            complement(4955..5041)
                     /locus_tag="PKM23_03925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5005..5007),aa:Ser,seq:cga)
     gene            complement(5078..5533)
                     /locus_tag="PKM23_03930"
     CDS             complement(5078..5533)
                     /locus_tag="PKM23_03930"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNY90653.1"
                     /translation="MQDHAAWMRLALAEARKALNKNEVPVGAVVVYEGRVIGRGHNLI
                     ESLQDPTAHAEMLAVTAAANTLASWRLDQSILYVTLEPCMMCTGAILLARIPLIVYGA
                     ADPRYGACGSALQMADGAKLDIQARVIGGIMKEESSLLLKEFFRKLRQN"
     gene            complement(5557..5630)
                     /locus_tag="PKM23_03935"
     tRNA            complement(5557..5630)
                     /locus_tag="PKM23_03935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5594..5596),aa:Arg,seq:acg)
     gene            complement(5674..5746)
                     /locus_tag="PKM23_03940"
     tRNA            complement(5674..5746)
                     /locus_tag="PKM23_03940"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5710..5712),aa:Glu,seq:ctc)
     gene            complement(5807..6661)
                     /locus_tag="PKM23_03945"
     CDS             complement(5807..6661)
                     /locus_tag="PKM23_03945"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNY90654.1"
                     /translation="MEKLLKEKIGEALAFLRPRLRERPRVAIILGSGLGRFSEQIEEA
                     IRIDTSEIPHYPHSTVPGHAGRWFAGRLGQLPVIAVQGRVHYYEGYPLAQVTFPVHLL
                     ASLGVEILIVTTASGGLNPAFSAGDLMLITDQLNFTFGNPLVGRPEDQLGPRFPDMMH
                     CYDQGLRDVARDAAARLGMRLREGVFCRVTGPAYETAAEVRMLRQLGGDAVSMSTAPE
                     VIVARQRHLRVLGISLITNPGTGLTAAKLTHEEVTATANQAGERMGQLLLEIARRLEG
                     EPTRPERG"
     gene            complement(6674..7258)
                     /locus_tag="PKM23_03950"
     CDS             complement(6674..7258)
                     /locus_tag="PKM23_03950"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNY90655.1"
                     /translation="MLSIVPKDTGWIEVICGSMFSGKTEELIRRLRRAQIARQKVAVF
                     KPVIDDRYSTEHIVSHNEGRLPSVPVSSARQILELAGEATVVGIDEAQFFGPELVDVC
                     QELADSGKRVIVAGLDQDYTGKPFEPMPQLLAVAEYITKTLAICVKCGNPANRTQRLS
                     HDSARVVVGAQDIYEARCRYCHEVFEADDENKGE"
     gene            complement(7292..8503)
                     /gene="udk"
                     /locus_tag="PKM23_03955"
     CDS             complement(7292..8503)
                     /gene="udk"
                     /locus_tag="PKM23_03955"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNY90656.1"
                     /translation="MPDLKHDPPCLIIGIAGPSGSGKSFIAEQILAALPAQRGLLLSQ
                     DDYYRDRSDLSLEKRQLINYDHPDAVEFSLLAEHLRTLRAGRAIEHPLYDFAVHNRRA
                     ERAEAGPADLVVVDGLLLYAVPEMLPLFDLKIYVDTPLDICFIRRLQRDVLERGRTME
                     SVIQQFCTTVRPMFIEHVLASRTQADLILSGEVPVEEVLPQILQRIQVLNPSLHSETS
                     GAAAPGPTWQELLMTEHLLLLRGLKLLEEAAAAVMQGDAERDRLERILNFLLSFGDRI
                     HNVKEENHLFPLMVENGISQEGLIKEMLLEHEAERELIARAMARLGKSGAAGAAEQEL
                     LAGHLRSYCARRCEHIRLENEKLYPRAAEVLGAGDIERMVADFAKVEERSLYPNSVRH
                     FSKQLAQWERS"
     gene            complement(8496..8909)
                     /locus_tag="PKM23_03960"
     CDS             complement(8496..8909)
                     /locus_tag="PKM23_03960"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNY90657.1"
                     /translation="MTKKRYDPVPLIRAAITASAGAKALYSRFQVGAALLCADGSVVT
                     GFNIESSSYGLTICAERVAMFRALSEGKESFTAIAIVAGSRNWCPPCGACRQVLWEWA
                     GEIEVILARSEEEYQILSLSELLPHAFDHGYLPNA"
     gene            complement(9040..9495)
                     /locus_tag="PKM23_03965"
     CDS             complement(9040..9495)
                     /locus_tag="PKM23_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNY90658.1"
                     /translation="MAFDSKGTLNKVMLIGRLGADPELKYTPAGAAVLTFSLATNTSW
                     KDQEGKSQEKTEWHRVVAWRKLAEVINQYAKKGGRVYVEGKVMTRSWTDKDGAKRYTT
                     EIQAESIQLLDSRGERENVESVDVPPPSEADFNGQGQHADSEVNDDLPF"
     gene            9672..10655
                     /locus_tag="PKM23_03970"
     CDS             9672..10655
                     /locus_tag="PKM23_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNY90659.1"
                     /translation="MKLTYLEAGLGEIDADDRFFQLLPGMADALLMRSVARLGIVEPI
                     HLQLRVGEESPAPRYRPVTGFRRLQAAAAAGLTRIPAMLLPRNWDDLQAYRWLVEQRG
                     ALRPFSPLEASHAIAALSGPLSLSREEILRTWFPLMGLAPNPKLFSLYAPLRLLEPEL
                     QSALAADELAMETAGAMASAEPAERLAFWQLSRTLRLGKNRQREFWLLLSDIERIDKA
                     SLPQLLAQPEWRSLLEEQNLTPSQKTDRFKNLLMTRRYPAYSATLARFESLLRSAKLP
                     PELHLRPTPWFAGEEYTVDFSFSTPAEFTARLETLQEMQARGLIDQLTELT"
     gene            10652..11737
                     /locus_tag="PKM23_03975"
     CDS             10652..11737
                     /locus_tag="PKM23_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA photolyase"
                     /protein_id="HNY90660.1"
                     /translation="MNASRYQPFLPDRLYIEEAVQNNPWTTRILARLSKAERIPIPDQ
                     QALPPAPAGKNILLSRQRGPFLRYCPGTSRHICCLYHNLDIAAGCDLGCSYCILQGYL
                     NVPMTTLYCNLDDMFVELDQVLDSHPDHFYRIGTGELSDSLTFDHFTAWSSDLVNYFR
                     QRRNAIIELKSKNTHIEHFISLPHDRRTVVSWSVNAAAIVASEEPHTPSLAERLEAAA
                     EAQAAGFWIGFHFDPMIRHEGWEEGYHEVVEQIFRRIEPRNIAWISLGALRYPPFLDE
                     IIRENHPESRIFLGELLPGADHKFRYFKPQRIEMFARMYRWIKAHGQETPVYLCMESD
                     EVWRKAFGWSPGSSAGLKRFLDERVGG"
     gene            11809..12429
                     /locus_tag="PKM23_03980"
     CDS             11809..12429
                     /locus_tag="PKM23_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNY90661.1"
                     /translation="MITEYISELAIKLLDAGGYWSAALLMMLESMVAPVPSEAVMPFV
                     GFLVADGKWNLSLSILTTTAGSIIGSLISYYMGYYGGKPVVLKVGKYLLLDRHDLELT
                     EKFFAKRSGTLTLFFSRFIPVIRHFISIPAGMGKMRLFPFLTATLIGATLWNSFLLFL
                     GIKLRENWTIVQKYSHQVDIVVLLLLAALVGWFIHSRLKRRREPHS"
     gene            complement(12477..13733)
                     /locus_tag="PKM23_03985"
     CDS             complement(12477..13733)
                     /locus_tag="PKM23_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HNY90662.1"
                     /translation="MSKAKQMAKLVGVIDGGREEVINLQRSLVAIPALAPESEGDGEK
                     AKADFMRSWLEKAEFDELIELNAPDPRVPCGYRPNMLCRLFGEERSRTVWVLAHLDVV
                     PAGDLKKWKSKPFELKLEGDELFGRGTEDNHQGLISGLLAVKAFKTAGIQPPCNLGLA
                     LVADEETSSRYGLDYLMTHHADQFGADDLVLVPDAGDEAGTLIEIAEKSILWLKVTVV
                     GKQCHGSTPELGNNAARAAAHLVVRMDGLEGIFDARNPLFEPPISTFEPTKHEANVPN
                     VNTVPGKEVFYFDCRVLPEYPLEEVEKKIRGMVKEVRDRFGVKIRLEFAQRAEAAPPT
                     PESAPVVVALKRAIREIKGVEPRCVGIGGGTVAAIFRRAGIPAAVWSSLMDTAHQPNE
                     SSRISDTLGDARVMAHVMLHALEAGK"
     gene            complement(13794..14498)
                     /locus_tag="PKM23_03990"
     CDS             complement(13794..14498)
                     /locus_tag="PKM23_03990"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNY90663.1"
                     /translation="MILAAAPPAYFPPPAACAKLAAADVVVCAESFRFSRKEGTHRTA
                     IRSMTGRRWLSVPVVGSGRPGTHISDLAIDFNQPWADEHWRVLEYNYHNAAYFYYYAE
                     AVERIIRGAGQSLVGLLAEAGQFVRGSLALRARVISSSLLPEVAERTGRLLAWAEACG
                     CDRYLIHPGEAGLLDLPRLEAAGIGLDRIEFTEAPYHQQGPGFLPGLSLLDLLFNEGP
                     AAAGYLQANIRIVPAD"
     gene            complement(14495..>15027)
                     /locus_tag="PKM23_03995"
     CDS             complement(14495..>15027)
                     /locus_tag="PKM23_03995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90664.1"
                     /translation="ARMMVDIMAGLKTSHPGLRLICLGPFNEAHVRAEVMEYARAQRV
                     DDIINYIPLVPHEEVADHLHRARVGLVPWQPHPQLLKLCYPNKVFEYMACGLPIVASD
                     LPGLRELITPASCGCLVAAEDVEAHIAAIRQLLDDPGAAEAMGLAGMAYARGHYSWEV
                     ERKSLLRLYEELGARR"
BASE COUNT         3025 a         4980 c         4137 g         2885 t
ORIGIN      
        1 cggggtgcct ccatccgggc cggcaatgcc ggtgacggag aggccatgat cggtcccgct
       61 cacccggacc accccttcag ccatcgcctc ggccacgggg gcgctgaccg ctccgtgcag
      121 ggcgatgagg tcttccggca cacccagaaa ctggatcttg gcctcattgc tgtaggtgac
      181 cagccctcgt tcaaaaaagc gcgagctgcc ggcgagattg gtgaacttgt tggcaatcag
      241 cccaccggtg cacgactcgg caaccccaag atgctcctgc cgactgatca gctcctcggc
      301 cagggcctgc tccaggggaa tgttgcgctc ggcgaagatg tagggacgaa tgttgcagca
      361 tacccgctcc acagccctcg ccattcgcgc gcgcacctcc tcctcatgct cgcccatggc
      421 attgaggcga atacgaaccc cggagggact gggcagaaag gcgaggcgga ccagggcctc
      481 gatttccgga atgccgccga tctgttcata gagggaactc tcggcgatgc cgatggtggc
      541 gatgtggtgg gtgaggatga cctgtccccc cttcgccttt tgcagctcag gcagtacaac
      601 cgccgtcatc atcgccttca tctcgtaggg aacccccggc atgaaataat aatgacggcc
      661 atcccgttca aagatatatc ccggcgccgt accgcggtca ttctgcatca accgcgccgt
      721 ttccggcacc agggcctggc tctcattgac gctggccatc ggcaggttgc ggcgcttgaa
      781 aagccttctg atgtggtcga gggtggcctc atcgcggatc aggcccacgc cgaaatattc
      841 acacgccgcc gtgcgggtga tatcatcgtt ggtcggcccc aaccccccgg tcaccaggat
      901 gtaatcagcc ctcccggcag cctcggccag ggcctgcttg atctggccga ggccgtcacc
      961 caccgttgtg acccggccca cttccatccc ggcctcaagc agctcctgcc cgatccaggc
     1021 tgcattggta tttatcgttt ggccgatgag cagttcatca ccgatggaga tcacctcaat
     1081 gcgcatccag cctcccgtcg gcctttcaga acagcctgaa ataaacaacc agctgcagga
     1141 cgatattggc gtaaatcccg gccacgatat catccgccat gatgccccag ccgtgcggca
     1201 gcctctccgc cgtgctcact ggcaccggct tgaggatgtc aaaaacacga aaggcgacga
     1261 aggccagaat aaagaccacc ccggtgcgag gcaaaaaaag cagcgagacc atcatgcccg
     1321 ccacttcgtc caggttcacc cggcccggat ccgtttcctg ccagagggcc tccgcccgac
     1381 cggccgccca gacgccgatc agggtggtaa gcagcaccag gccgagcagg aggaatccct
     1441 gatccatgcc gacgaaccag agcagcagaa cagctgcagc cgcgcccaca gtccccgggg
     1501 cgacaggaaa ataaccggtg tataaagtcg tggctaaaaa acgcgccacc tcatcacgcc
     1561 actgcctctt ctcgttcaca gagtttccct gaataatacg taaggccgga agagattccg
     1621 accttatcca tttcctactg caaattacta aaaaagtgtc aataaaggca agtgataaat
     1681 tggtgcggcg ggcggtgccg gggagggtta taacgggacc cgcccggcca tggcgcgcga
     1741 aagggtcatc tcgtcggcgt actcgatgtc tgcgcccacc gggatgccgc gggcgatgcg
     1801 cgaaacccgc acctgcatgg gtttgaaaag acgggccagg tagagggcgg tggcctctcc
     1861 ctcggtgttg ggattggtgg cgagaatgat ctcctcgacc tcttcgctca accgcccgag
     1921 aagctccttg attttcagat catccggccc gatgccgtcc agaggggaga gcgcaccccc
     1981 gaggacgtga taaagcccct tgaattctcc ggtcttttcc agagcgacca cgtcattggc
     2041 ctcctctaca acgcaaatga gacggcggtc ccgcatctca tccgcgcaga tcggacaggg
     2101 atcctcttcg gtgatattga aacagcgcga gcagtagcgc accttctcat gcagctcggc
     2161 gagggagccc gccagggcgc gtacctcctc ctcaggcacc ttgagcagaa agaagaccag
     2221 gcgctgggcc gatttgcgtc cgatgcccgg cagcttgctc agctcatgga tggctcgttc
     2281 aaccgcctcg gatgaatagt tcaccgccag acctcacaca tcagagcgtt tctcacatac
     2341 ccggcaattt gaggccgccg agcatcccgc ccggatccat gccgccggtg actttagcca
     2401 tctcctcttc cgctttggcg cgcccgtttt gcagggcttg attgaccgcg gccaggacca
     2461 gatcctccag catctcaaca tcctccggat cgaccacctc cggattgatc tttacctgca
     2521 ggatctcctg cgcggcgttg gcagtgactg tgaccatgtt gccgccggcc gtgccctcaa
     2581 ccctcagatt ggccaggttg gcctgcattt tctgcagatc ttcctgcatc ttctgcgcct
     2641 gacgcagaag gccgcccatg tttcccttgt tgaacataaa ccacctctgt cggctatttg
     2701 atgaattcag cgtcgaactc ttgcactatt ctttgcatac ggacatcatc ccgcagcttc
     2761 ttctcgaact cgctcttgcg atcggtcagc gccgggattt tacgcacccg cggcaggatc
     2821 ccctcctcat ctttcacaca caggagccgc agcggaaccc ccagaatccc ggtcatgatc
     2881 tcctgcagaa gcaggcggtt gttttcgacc gcattcatct ggaaactgct ctcacggccg
     2941 aacatgatct ccagctgccc tgcgcccacc ttaaccggcc agccctcctg caaaaaagtg
     3001 ccaagcgcga ccttctgggt gcgcaccgca tcgagcacac cctgccagcg ctcttccacc
     3061 gcagctaaag agagagcggg cgcctgctca accaccgctt tctgctccgg cgaaactgca
     3121 tcggcaggaa tcaaagcggg gccggcgggc gcagatgcag aaggtgcggc cgaaggacgg
     3181 gaagtggtcg ggttcggagt ttgggcgaga aaagagggct tgtagccagg ggaggtggaa
     3241 ctgctcaagg ggagggaatt agactttttt tttacctctc ccagctgctc catgagggta
     3301 gtcagctgca cgctgctgct catccgcacc atcttgacca ggccgacctc gaaatggagc
     3361 cgggcattgc tgctgcggcg caccaggttt tcggcatccg ccgccacctt gatcaaccgc
     3421 agcagatcct cgatggaaaa ggcctctatc tgctcccgat agcgcacaat atgctcctcg
     3481 gaacagtcaa tgagatgagg atcgctgccg cttttcacca ccagaaaatt gcgcagatgc
     3541 tcggcaaggc cgatgagaaa ttcgtcaaag tcgaagccct ccgcaaaaag atggtcggca
     3601 agcttgatag cgccatccac gtccccggcc tgaatcagat ccgtgacccg aaaaaagagc
     3661 tcctggtcga tgatcccgag cagcgaggcc acatcccggg cctggatgtt tttaccggcg
     3721 aaggcaatga tctggtcgag aatactctgc gaatcccgca tactgccgtc ggccttgagc
     3781 gcgatcatgc gcagtgattc cgcatcgatc gcgatctcct cttgacggca catcgtctgc
     3841 agctgctcga caataaactg atgggacatg cgcttgaaat cgaagcgctg gcagcgcgac
     3901 aggatcgtcg ccgggacctt atgtgattcg gtggtggcaa agatgaacaa cacccgcgcg
     3961 ggcggttcct ccagggtctt caacagcgcg ttgaaggcct ccttggtgag catgtgcact
     4021 tcatcgatga tatagatgcg gtatttgccg ggatggggcg cgtagcgcag cccctcgcgc
     4081 agattgcgca cctcgtcgat gccgcgattg gaggcgccgt caatctcgaa tacatcgagg
     4141 ctgcggcttt cattgatctc tatgcaggaa gtacaggtgt tgcaggggtt gatcgtgggg
     4201 ccctgatcgc agtttagggc cttggcaagg agacgagcaa ccgtggtctt gccaatgccg
     4261 cgcggcccgg aaaaaaggta ggcattggca atccggttct gttggatggc attctgcagg
     4321 gtgtgggtca catgctgctg ccccagaacg tcggcaaaaa acatggggcg atattttcgg
     4381 gcaagcacca agtaggacat ggagggatcc ggcttcggtc acgtcaaaaa aaggctgtgc
     4441 acccaccttc gacccccctg cgcaccagac gacacagcgc acaactccag ccaagtactc
     4501 ctacggcaca tagagaatgt gcttgccgct gctatcttcc gatcctgacg gggttgggca
     4561 cgcatctatt gcgtagggct tgggctttca gcattgcaca ccatggcgag acaagaacgt
     4621 gagcaggcct cagaatggga attcgaccct gctggagcgg gttgcaggtt acagggcacc
     4681 gctagctccc cgtctagcac agcctcttgt gtggagctga tgggattcga acccacgacc
     4741 tatacgttgc gaacgtatcg ctctcccagc tgagctacag ccccgaaaga tttgcagcag
     4801 gatgacgccg gccccgccgc acgagcgggc agaagacctc tggcggagag ggcgggattc
     4861 gaacccgcgg taggggtcgc ctacacacgc tttccaagcg tgctccttag gcctctcgga
     4921 cacctctccg aaacaacagc ctgatctcca tctgcggaga ggcagggatt cgaaccctgg
     4981 gtacccgcaa gggcacaacg gttttcgaga ccgccccgat cagccgctcc ggcacctctc
     5041 ctgaaactca tggcccgctc ctcgctggaa agaggggcta attttgacgc agcttgcgaa
     5101 agaactcctt caagagaaga ctgctctcct ctttcatgat cccgccgatg accctggcct
     5161 gaatatccag cttggcgccg tcagccatct gcagtgcact cccgcacgcc ccgtaacggg
     5221 gatcggccgc accataaaca atcagcggaa tcctggcgag caggatcgcg cctgtgcaca
     5281 tcatacaagg ttcgagggtg acatacagga tggattgatc cagccgccag gaggcgagag
     5341 tattggctgc ggcagttacg gccagcatct ccgcatgcgc ggtgggatcc tgcagggatt
     5401 cgatgagatt gtgccccctg ccaatgaccc ggccctcata caccacaacg gcaccgaccg
     5461 gaacctcatt cttattcagg gccttcctgg cttcggcaag cgcgagccgc atccaggctg
     5521 catgatcctg catgaggaga gccttcccaa aaaatgcgcg cccggaggag ctcgaatccc
     5581 caaccttctg gtccgtagcc agatgctcta tccagttgag ctacgggcgc gttatgttca
     5641 gagaacatct tgctgtgcag tacttgaatt ttgtagcgtg tacgggattc gaacccgtgt
     5701 taccggcgtg agaggccagc gtcctagacc cctagacgaa cacgccaaaa tcccaagctc
     5761 ataaatatac gcaatttcaa taatattgtc aatatttttc agtaaatcat ccccgctccg
     5821 gccgggttgg ctccccctcc agacggcgag cgatctcgag caggagctgc cccatccgct
     5881 cccccgcctg attggcggta gctgtgacct cctcatgggt aagtttggcc gccgtcaggc
     5941 ccgtgccggg attggtgatc agggagatgc cgagcacgcg cagatgacgc tgccgcgcga
     6001 cgatcacctc cggggcggtc gacatcgaga ccgcatcccc cccgagctgg cgcagcatgc
     6061 gcacctccgc cgcagtctcg taggccgggc cggtcacccg gcaaaaaacc ccctcgcgca
     6121 gcctcattcc cagcctcgcc gccgcatccc gcgccacatc acgcagcccc tgatcgtaac
     6181 aatgcatcat atccggaaag cgcggcccga gctgatcctc cggcctgccc accaggggat
     6241 tgccgaaggt gaaattgagt tgatcggtga tcagcatcag atccccggcg gaaaaggcgg
     6301 gattcaatcc cccgctcgcc gtggtgacga tgagaatctc cacacccaga ctcgcgagca
     6361 gatggaccgg aaaagtcacc tgcgccaggg ggtagccctc ataatagtgg acccggccct
     6421 gcacggcgat gacaggcagc tggcccagac ggccggcgaa ccagcggccg gcatgtcccg
     6481 gaacggtcga atggggatag tggggaatct ccgatgtgtc gatcctgatt gcctcttcaa
     6541 tctgctcgga aaagcggccg agccccgagc cgaggatgat cgctacgcgc ggccgctccc
     6601 tgagccgggg acggagaaag gcgagagcct caccgatctt ttccttcaac agcttttcca
     6661 cgacaggcgt ccttcattct cccttgttct cgtcatccgc ctcaaagacc tcgtggcagt
     6721 agcggcagcg cgcctcatag atatcctggg cgcccaccac tacccgcgcg gaatcgtggc
     6781 tcagacgctg cgtccggttg gcggggttgc cgcatttgac gcagatggcc agggttttgg
     6841 tgatatactc agcgaccgcc agcagctgcg gcatgggctc aaaaggctta ccagtataat
     6901 cctgatccag accggcaaca atcacccgct tgccgctgtc cgccaactcc tggcagacat
     6961 cgaccagctc cggcccgaag aattgggcct catcaatgcc gacgactgtg gcctcgcccg
     7021 ccagctccag gatctgccgg gccgaagaga ccggcaccga aggcaggcgg ccctcgttat
     7081 gtgaaacgat atgctcggtt gaatagcggt catcgatcac aggcttgaaa acggctacct
     7141 tctgacgggc gatctgggct cgccgcagcc tgcgaatcag ctcctcggtc ttgccgctga
     7201 acatgctgcc acagatcacc tcgatccacc cggtatcctt cggaacgata cttaacatgc
     7261 tcctctcctg aaagctaatt attccgcccg ctcaagaccg ctcccactgc gccagctgtt
     7321 tggaaaaatg gcgcacggag ttgggataga gcgaacgctc ttccaccttg gcaaaatcag
     7381 cgaccattct ttcgatgtcc cctgcaccga gcacctccgc agcgcggggg taaagttttt
     7441 cattctccag acggatatgc tcacacctcc ttgcgcagta gctgcgcaga tggccggcga
     7501 ggagctcttg ctcggccgca ccggctgcac cggacttgcc aagacgggcc atcgcccgcg
     7561 caataagctc ccgctccgcc tcatgctcca gcagcatctc cttgatcaac ccctcctggg
     7621 agatgccgtt ctcgaccatc aggggaaaga gatggttctc ctccttgaca ttgtgaatcc
     7681 ggtctccgaa ggagagcaga aaattgagga tccgctccag tcgatcccgc tccgcatccc
     7741 cctgcatcac tgctgccgcc gcctcctcca gcagcttcag gccgcgcaag agcaggaggt
     7801 gttccgtcat aagcagctcc tgccaggtcg ggccgggcgc cgccgccccg gaagtttccg
     7861 aatgcaggga gggattgaga acctggatgc gctgcaggat ctgaggcagc acctcctcga
     7921 ccggaacctc gcctgaaagg atgagatcgg cctgggtccg gctcgcaagc acatgttcga
     7981 tgaacatcgg ccgcaccgtc gtgcagaact gctggataac cgactccatg gtgcgcccgc
     8041 gctccaggac gtcccgctgg agccggcgga tgaagcagat gtccagggga gtgtccacat
     8101 agatcttgag gtcgaagagg ggcagcatct ccggcaccgc atagagcagc agcccgtcga
     8161 ccacgaccag atccgctgga cccgcctcgg cccgctccgc cctgcgattg tgcaccgcaa
     8221 aatcgtagag ggggtgttcg atcgcccggc ccgcgcgcag ggtgcgcaga tgttcagcga
     8281 gcagggaaaa ttcgaccgca tccggatgat catagttgat gagctggcgc ttctccaggg
     8341 acaaatccga acggtcgcgg tagtaatcat cctgcgaaag caggaggccc cgctgggcgg
     8401 gcagcgcggc caggatctgt tcggcgataa aactcttgcc cgatccggag ggaccggcga
     8461 tcccgatgat caggcagggc ggatcatgtt tcaggtcagg cattgggcag atatccatgg
     8521 tcaaaggcat gcggcaacag ctcgctcagg gagaggatct ggtactcctc ttcgctgcgg
     8581 gccaggatca cctcgatttc ccccgcccat tcccacagca cctgccggca ggccccgcag
     8641 gggggacacc aattgcggct gcccgcaacg atggcgatgg ccgtgaagga ctccttgcct
     8701 tcggaaaggg ccctgaacat cgccacccgc tctgcacaaa tggtcaggcc gtaggaacta
     8761 ctctcaatat tgaatccggt cacgaccgaa ccatccgcgc agagcagggc cgcgccgacc
     8821 tgaaaacggg aatagagggc cttcgccccg gcgctggcgg tgattgccgc gcgaatcaac
     8881 ggcaccggat cgtacctctt ctttgtcatc gcttcacctg caaggaataa aatgggctgc
     8941 ggaaaaaaaa atccaaagcc gcactggacc ttggattttt acacactcaa accggcccga
     9001 atcgttggca attcccaccg tcccgaccgg agactgcact taaaaaggca gatcgtcgtt
     9061 gacttcgcta tcggcgtgtt gtccctgtcc gttgaaatcg gcctcgctcg gcgggggcac
     9121 atccaccgac tccacattct cgcgctctcc ccggctgtcc agcagctgaa tggattcagc
     9181 ctggatctcc gttgtatagc gcttggcccc gtccttgtcg gtccaggaac gggtcatgac
     9241 cttgccttcc acatagaccc gcccaccctt cttggcgtac tggttgatca cttcagccag
     9301 cttgcgccag gccaccacgc gatgccattc ggtcttttcc tgggatttcc cctcctgatc
     9361 cttccacgag gtgttggtag caaggctaaa agtcagaacg gcggctccgg cgggggtgta
     9421 cttcaattcg ggatctgcgc ccagtcttcc gatgagcatg actttgttca aggtgccttt
     9481 actatcgaaa gccatttgac gcctccttgt tataatgtaa acagcctttt tggcccgcct
     9541 cagtttatct tactaataat aaacaaaatt gtcaagggaa tttatcgcca caaaaacgcc
     9601 aaaccgtatg aaaagacttg aaataaaggc ccaattatcg ctacattatt cttctccaat
     9661 gaaaggatgg tatgaaactg acctatcttg aagccggcct tggcgagatc gacgccgatg
     9721 accgcttctt tcaactgctc ccggggatgg cggatgccct gctgatgcgc tcggtcgccc
     9781 gcctcggcat cgtcgagccc attcacctcc agctgcgtgt tggtgaggag agtcctgcac
     9841 cacgctaccg accggtcacg ggattccgcc gcctgcaagc cgccgcagcc gccggcctca
     9901 cccggatccc ggcgatgctt ctgccccgga attgggatga cctgcaggcc tatcgctggc
     9961 tcgtcgagca aaggggcgct ctccgcccct tctctcccct tgaggcctcc cacgccatcg
    10021 ccgccctctc cggccccctc tccctgagcc gggaggagat cctgcgcacc tggttccccc
    10081 tcatgggtct ggcccccaat cccaagctct tcagcctcta cgcccccttg cgcctccttg
    10141 aaccggagct gcaaagcgcc ctcgccgccg atgaactcgc catggaaacg gccggcgcca
    10201 tggcctccgc tgagccggca gaacgcctgg ccttctggca gttgagcagg accctcaggc
    10261 tcggcaaaaa ccgccagcga gaattctggc tcttgctctc cgatatcgag cggattgaca
    10321 aggcctccct gccccagctt ctcgcgcagc cggaatggcg gagcctgctc gaggagcaga
    10381 acctcacccc ctcccaaaag actgaccgct tcaagaacct gctcatgacg cggcgctacc
    10441 ccgcttacag cgccaccctc gcccgcttcg agagtctgct ccgatccgcc aagctgcccc
    10501 cggaactcca cctgcgcccg accccctggt tcgccggcga ggagtacacg gtcgatttct
    10561 ccttctctac ccctgcggaa ttcaccgccc gcctcgagac cctccaggag atgcaggcgc
    10621 gaggcctgat cgaccaactg acagagctga catgaacgcc tctcgctatc agcccttcct
    10681 ccccgaccgc ctctatatcg aggaggccgt gcaaaacaat ccctggacga cgcgcatcct
    10741 cgcccgactg tccaaggccg agcgcatccc catccccgac cagcaggccc ttccaccggc
    10801 cccggccggg aaaaacatcc tgctcagccg ccagcgcggc ccctttctgc gctactgccc
    10861 aggcacctcc cggcatatct gctgcctcta tcacaatctc gacatcgccg ccggctgcga
    10921 tctcggctgc tcctactgca tcctgcaggg ctacctcaac gtccccatga ccaccctcta
    10981 ctgcaatctc gacgacatgt tcgtcgaact cgaccaggtg ctcgactcgc atccggacca
    11041 tttttaccgc atcggcaccg gcgaactctc cgacagcctg accttcgacc acttcaccgc
    11101 ctggagcagt gatctggtga actatttccg ccagcgccgc aacgccatca tcgaactcaa
    11161 gagcaagaac actcacatag aacacttcat cagccttccc catgaccggc gtacggtcgt
    11221 ctcctggtca gtcaatgccg ctgccatcgt tgccagcgag gagccccata ccccgtccct
    11281 cgctgagcgc ctcgaagcgg cggccgaggc ccaggcggcc ggtttctgga tcggcttcca
    11341 cttcgacccc atgatccgcc acgaaggctg ggaagagggc taccacgagg tcgtcgagca
    11401 gatctttcgc cggatagagc cccgcaatat cgcctggatc agcctcggcg ccctgcgcta
    11461 tccccccttc ctcgatgaga tcatccggga aaaccacccc gagtcgcgga tctttctcgg
    11521 cgagctgctc cccggcgccg accacaagtt ccgctatttc aagccgcagc gcatcgaaat
    11581 gttcgcccgc atgtatcgct ggatcaaggc gcatggccag gagaccccgg tctatctctg
    11641 catggaaagc gacgaggtct ggcgcaaggc cttcggatgg tctcccggca gttcggccgg
    11701 gctcaaacgc tttctcgatg agcgggtggg tggctgagat aaaaatgcgc agatcatctc
    11761 cattgtccgt actaattgaa aatcgatccc ctgaaccgga gaaacccaat gatcacagag
    11821 tatatttccg aactggccat taaactgctt gacgcgggcg gctactggag cgccgccctg
    11881 cttatgatgc tcgaaagcat ggtggcgcct gtccccagcg aggccgtcat gcccttcgtc
    11941 ggattcctcg tcgccgacgg taaatggaac ctctccctct ccattctgac caccacggcg
    12001 ggctccatca tcggctcgct catctcctac tatatgggct attacggagg taaacccgtg
    12061 gtgctcaagg tcggcaaata cctcctcctc gaccgccacg accttgagct cactgaaaaa
    12121 tttttcgcca aacgcagcgg caccctcacc ctctttttca gccgcttcat ccccgttatc
    12181 cgccacttca tctccatccc ggcgggcatg ggcaaaatgc gcctcttccc cttcctgacg
    12241 gcgaccctga tcggggccac cctctggaac agctttctcc tctttctggg catcaagctg
    12301 cgcgaaaact ggactattgt gcaaaaatat tcgcaccagg tcgatatcgt cgtgctgctc
    12361 ctgcttgccg ccctggtggg atggttcatc cactcccgcc tcaaacgccg cagagagccg
    12421 cactcctgat caccgccatt taaaaaggcc cgccggattg ttccggcggg cctttttcat
    12481 ttcccggcct ccagggcgtg gagcatcaca tgcgccatca ccctggcatc ccccagggtg
    12541 tcggatatcc tggaggattc gtttggctgg tgtgcggtat ccataaggct ggaccagact
    12601 gcagcgggga tgccggcgcg gcgaaaaatg gccgccaccg tgccgccacc gatgcccacg
    12661 cagcgcggct caaccccttt gatctcccga atcgccctct tcagagccac gaccaccgga
    12721 gcactttccg gcgtgggcgg cgccgcctcg gcgcgctgtg caaactcgag cctgatcttc
    12781 accccgaaac gatcacgtac ctccttcacc atcccccgga tttttttctc cacctcctcc
    12841 agggggtact ccggcaagac ccggcagtca aaataaaaga cctccttgcc cggcacagtg
    12901 ttgacgttgg gcacattggc ctcatgcttg gtcggctcga aggtgctgat ggggggttca
    12961 aagaggggat tgcgcgcgtc aaagatccct tccagaccat ccatcctgac caccaggtgg
    13021 gccgccgcgc gagcggcgtt gttgcccagc tccggggttg aaccatgaca ttgtttgccg
    13081 acgacggtca ccttgagcca gaggatgctc ttctccgcga tttcgatcag cgtaccggcc
    13141 tcatccccgg catccgggac cagcaccaga tcgtcagcgc cgaactggtc ggcgtgatgc
    13201 gtcatcagat agtccaggcc gtagcggctc gaggtctcct cgtcggcgac cagggccagg
    13261 ccgagattgc aagggggctg gatcccggcc gttttaaagg ccttcaccgc cagcaggccg
    13321 gagatcaatc cctgatggtt gtcctccgtc ccgcgcccaa aaagctcatc cccttccagc
    13381 ttcagttcga aaggcttgct cttccacttt ttcagatccc cggccggcac cacatccaga
    13441 tgcgccagca cccagaccgt gcggctgcgc tcctccccga agagccggca cagcatgttt
    13501 ggacgatagc cgcacggtac ccgcggatcc ggggcgttta gctcgatcag ctcatcgaat
    13561 tccgctttct ccagccagct gcgcatgaaa tcggcctttg ccttctcacc gtccccctcg
    13621 ctctcgggcg ccagggcggg aatggccacc aggctgcgct gcaagttgat gacctcttcc
    13681 ctcccgccgt cgatcactcc aaccagcttt gccatctgct tcgctttgga catagatcct
    13741 ccctcgattt atatagccgg atcaatatca ctctatgata aaattgcggc cgctcaatcc
    13801 gccggcacaa tcctgatgtt ggcctgcagg tagccagcag ccgccgggcc ttcattgaaa
    13861 agaagatcaa gaagcgagag cccgggcaga aagcccggac cctgctgatg atagggcgcc
    13921 tcggtgaatt caattcggtc caggccgatg cctgccgcct cgagccgcgg cagatccagc
    13981 aggccggctt caccgggatg gatcagatag cgatcgcatc cgcaggcctc cgcccaggcc
    14041 agaagtcgcc cggtgcgctc cgccacctcc ggaagaagac tgctggaaat cacccgggcc
    14101 ctcagagcga ggctgccccg gacaaattgg ccggcctcgg ccagcagccc gaccaaagac
    14161 tgcccggcac cgcggatgat cctctccacc gcctcggcat agtagtagaa ataggcggcg
    14221 ttatgatagt tgtactccag aacccgccaa tgttcatccg cccagggctg attgaagtcg
    14281 atggcgagat cgctgatatg ggtcccgggc cgcccgctgc cgaccaccgg caccgaaagc
    14341 cagcgccggc cggtcatgga cctgatcgcc gtccggtggg tgccctcctt gcggctgaag
    14401 cgaaagcttt cggcgcaaac caccacatcg gcggccgcca gcttggcgca ggccgccggc
    14461 ggcgggaaat aagctggggg cgctgccgcc aggatcatcg ccgcgccccc agctcctcgt
    14521 agagcctgag caggctcttg cgttccacct cccagctgta atggccgcgg gcataggcca
    14581 tgccggccag ccccattgcc tccgccgcac cggggtcgtc cagcagctgc cggatcgccg
    14641 caatatgggc ttccacatct tcggccgcaa ccaggcagcc gcagctcgcc ggggtgataa
    14701 gttcgcgcag cccgggcaga tcactggcca caatcggcag gccgcaggcc atgtactcaa
    14761 agaccttatt gggataacag agcttcagca gctggggatg gggctgccag ggcaccagtc
    14821 cgacccgggc gcggtggaga tgatccgcca cctcctcgtg cggcacaagg ggtatgtaat
    14881 taatgatatc atcgaccctt tgcgcccggg catattccat aacctctgcc cgcacgtgcg
    14941 cttcgttaaa ggggccgagg cagatcaacc ggagtccggg atggctcgtc ttcagcccgg
    15001 ccatgatatc aaccatcatc cgcgccc
//
LOCUS       DAOPWU010000042        14984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4066_length_14984_cov_5.599705, whole genome shotgun sequence.
ACCESSION   DAOPWU010000042 DAOPWU010000000
VERSION     DAOPWU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4066_length_14984_cov_5.599705"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1187)
                     /gene="tal"
                     /locus_tag="PKM23_04000"
     CDS             complement(93..1187)
                     /gene="tal"
                     /locus_tag="PKM23_04000"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNY90665.1"
                     /translation="MAELLARGQSIWIDYIEREFITGGRLAGLIGAGVRGLTSNPAIF
                     QKAISAGKAYDAEIAALSARGLTGAPLYEALAIADIRAAADAFRPLYESSAGMDGYVS
                     LEVSPALAHEAEKTILEAKRLAAAVERPNLMIKIPATPAGIIAIPEVVAAGISVNATL
                     IFNTRHYRQVAEAWFTGLEMLLAQGRSLPGLSSVASFFVSRLDTKLDPLLAARGFAQW
                     QGKMAVANAAATYDLFLELHRSPRWQRLAEKGARMQRLLWASTGTKNPRYSETLYVDD
                     LIGTGTVNTIPPATLQAWLDHGRTAAALPVNPALSSAALSALAEIGIDLDGATEELQQ
                     DGVAAFAGSFAAMLTSVADKEKSLRPAHSS"
     gene            complement(1233..1949)
                     /locus_tag="PKM23_04005"
     CDS             complement(1233..1949)
                     /locus_tag="PKM23_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNY90666.1"
                     /translation="MRRRKLFFHRGLCLLLLTAAPLPAAEWGGNYPDHIWQGTTAAFN
                     NPRWLFAAGAVAGLSAWDRDLQAAHPQLMPAGLARFGKYYGFGTNYLAGSLFILTDGL
                     GGWPMSRRERLDSWQSFSEAYAANMAATGFLKLATHRLRPDGSSRDSFPSGHASASAC
                     VAAHLQRRYGAAAGLPAAAAALITGASRLQHERHWLSDVLAGALLGGLIGDGFGQLER
                     SSGQNKQARTRWQVDLLLIF"
     gene            complement(1946..2980)
                     /gene="fni"
                     /locus_tag="PKM23_04010"
     CDS             complement(1946..2980)
                     /gene="fni"
                     /locus_tag="PKM23_04010"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNY90667.1"
                     /translation="MSEEINHRKYDHIRIVRDDDSTDRGKSYFDAWPLQHRALPEIDL
                     KEIDPSTRFLGKPLSFPLMISPMTGGDHDLVRAINKNLAKAAEATGVAMAVGSQRVMI
                     THPAARESFALRPFAPKTVLLANLGAVQFNYGFGLRECQQALEILGADGLYLHLNPLQ
                     EAIQPEGETDFRGLADKIGAIAAALPRPLLLKEVGSGMSLRDAEMAVARGIRYIDVAG
                     SGGTSWSRIEHFRHRPGRSSDGGRAGLLFQDWGIPTPDLLRQLSPLRDRVTLIASGGI
                     RSGIDMAKALILGASLCGIAKPFLEPALRSADEVIQLIEQLRKEFIITLFLLGIRRLD
                     ELIGNESLLA"
     gene            3164..3778
                     /locus_tag="PKM23_04015"
     CDS             3164..3778
                     /locus_tag="PKM23_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90668.1"
                     /translation="MEIHDLLYERVSEPEPFLFECWEPEGVAVVIGHGQNAAQEADLT
                     ACRRDGVVVIRRRGGGGAVVLMPGVLCLSCAFLSGKSDSPWFYFQQINLFIIDLLARR
                     FGLEGLEPAGISDIALEGRKILGCSMFKSRHLYFYQGSMLVNPDLFRISTYLIHPSRE
                     PGYRRGRSHSDFVTSLWLSGYPLPINNLRQAFSGEAGALRTLLI"
     gene            complement(3791..4954)
                     /locus_tag="PKM23_04020"
     CDS             complement(3791..4954)
                     /locus_tag="PKM23_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNY90669.1"
                     /translation="MRALLPSRRPQIRPRSLRLGVLLLALLFLAGCGRFTWKPAAAAP
                     ELVWPQLGGSPARNAVVPDEPCFPLRQLWMKRATGGINPSLIGFGEWLLYATKDGKIE
                     GFNLRSGAKPDRILGRNNTALTCVWSGDGLVLVKRLRADNLLFYDLDRGRMRWQRSIG
                     PVMGEPLVAGGRLYLATLRGRLLAIALEDGAILAEASLSHDCLAAPAMSDTVLAVGDD
                     RGLLHAFNSRLESLWQYETGGALRAPAVAGGGLFYIGSTSGRFSAIDAVSGAERWRFE
                     SPGKILYAAAVSDSLVLFASTSNLVYGLDRTDGRLVWQFDLGAVPAAAPLICGSTLFI
                     GAANKKLYALSLADGRELWSFTAKGRISTSPVVFGGRLIFAAGYDYLYCFALR"
     gene            complement(4957..7884)
                     /locus_tag="PKM23_04025"
     CDS             complement(4957..7884)
                     /locus_tag="PKM23_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNY90670.1"
                     /translation="MSGARFLHILRRSLLLLAALAGLIPGGAGAQYYFGRNKVIYNHF
                     DWRILQTDHFDIYHYPEMRELTEIGAACAEESYRFLENKFGISLTERIPLIFYATHAH
                     FQESNTISYLIPEGVGGFFEFLKGRVVVPANGSIPEFKRVIRHELVHVFTHARVNRVL
                     REHKQRTHPGLPLWFIEGLAEYWSAGWDSEAEMFLRDAVINNYLCPVTGIYQINGTFL
                     MYKEGQSLLRFIDQGWGEDKILQLIENVWVSPYFSEVIKLTLGIDLREFDERWNYALK
                     KEKYPLLGENDAASRVTTPITRQGINIKPAYYEGDSAQVVFISNRTGYANIYLAALAE
                     AEKEPPMEVVVAGERSEAFESFHMLNSAIDVNGRGELAFISKSGERDLLYIMELPEKK
                     ITGRHGFEKLVSLFSPSWSPDGRRLVFTGLAFSGRRDLYLYDRESGELTSLTDDLFDD
                     KDPAFSPDGRLIAFSSDRGAGGAKGGYNLFIYDLAGGDITPLTRGEAHDQMPAWSPDS
                     SCIAFVSDRSGTQNIWVVESPAAAAPSLAERLGLQPGAGAAPLHLPAPRQVTRFITGA
                     YDPEWTPQGDLLFTVFEKFAFNISLERDLAARLRAAQQPAAAEEAPVDTLAGTAWSYE
                     RISGEGEGREIRYRSKFSLDIAQSQITQDPIFGTSGGAQMVVSDMLGNYQYFFLLYNT
                     ARVQSELLSSFNFALSRVDLSHRTNFAVGLFHFAGRYYNRYDLWFWERRYGGYTALSY
                     PLSKFDRIEASLNVRSSDKEWYADGYRRKALLVSNFVSYVQDNSLWGPTGPLDGSRIN
                     LTLGTTVDVANANVRFGTAIIDYRRYFRLSTQAAHALRLWTQINHGKEATPFVMGGSW
                     DLRGYRFWRLWGTKVALLSNELRFPFIDRFSLKFPFGGVAISSIRGALFADVGNAWDE
                     RLEELLGSAGFGVRFQLGGMLVLRFDTGRKFLIRDAGRFYQPRQYDFEKALFTQFFFG
                     WDF"
     gene            complement(7963..9081)
                     /locus_tag="PKM23_04030"
     CDS             complement(7963..9081)
                     /locus_tag="PKM23_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNY90671.1"
                     /translation="MENIRIQRITTPGERKQFIKFPWRIYRHDPLWVPQLIGDEMKIL
                     DPAKGTFYTLGEIALFMAWRGSEPVGRISAQINHQYEHYHDNHTGFFGFFESIEDQQV
                     ADALVHAAAEWLRSKGKKRMMGPMSPSMYDISGTLIDGNDTMPCILTAYNPAYYERLM
                     DSAGMIKAMDWYAFLVSRDSQIRPGLQRIADRVMHQEGLEIVPLDMKIFDQRVREVAR
                     IFSDAWMENWGHVPYTDRQVEDLVKELKMVIVPEVTLFALYHGECIGFSLSVRDVNPA
                     LQKANGRLFPFGLLKLLWHVRKSTRLRTMAMGVLKEHRHRGIDIAFYLKTFEEGLKLG
                     FVDSECSIILEINQRMIGALEDLSARRYKTFRFYEKEI"
     gene            9205..9459
                     /gene="rpsT"
                     /locus_tag="PKM23_04035"
     CDS             9205..9459
                     /gene="rpsT"
                     /locus_tag="PKM23_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNY90672.1"
                     /translation="MANHKSAVKRIQTNTRDNERNKSYKNTLRTSVKKVRAVTSKEEG
                     EKLFRSTASLLDKLAKKGIIHKNKAANQKAKLASLINKLS"
     gene            complement(9605..11074)
                     /gene="guaB"
                     /locus_tag="PKM23_04040"
     CDS             complement(9605..11074)
                     /gene="guaB"
                     /locus_tag="PKM23_04040"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNY90673.1"
                     /translation="MNKIIGEGYTFDDVLLVPNRSDVLPKETDVRTRLTRKITLNIPL
                     LSAAMDTVTGADLAIAIAREGGIGILHKNCSIQEQAAEVDKVKRSEAGMIMNPITLAP
                     DRKLSEALELMQRYRISGIPIVDGQKLTGILTNRDLRFETDLNKRVSSLMTGKERLIT
                     APVGTSLDEAEKLLQQHRIEKLLVVDKEGKLKGLITVKDIQNRKRFPFASKDHHGRLL
                     VGAAIGVAHDTQERAEALIEAGADVLVVDTAHGHSHGVIQTVALMKKQFPKIEVIAGN
                     IATPEAAEELIKAGADAVKVGIGPGSICTTRIVAGTGVPQLSAIMSCAEICRKLKVPL
                     IADGGIKQTGDIAKAIAAGADTVMLGNMLAGTEESPGETILYEGRSYKVYRAMGSLGA
                     MRSGRGDRYFQEGIQDAKKLVPEGIEGRVPYRGKVADVIYQMIGGLRSAMGYSGAATI
                     SDLQEKGRFVRITQAGLLESHPHDIIITKEAPNYMIQGR"
     gene            complement(11139..12701)
                     /gene="hutH"
                     /locus_tag="PKM23_04045"
     CDS             complement(11139..12701)
                     /gene="hutH"
                     /locus_tag="PKM23_04045"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNY90674.1"
                     /translation="MQPILLDGHTLTLADLHAVVSGRLVAITTAAMARMTASRACIEG
                     IIAEGRVVYGVNTGFGKLANIHIPDSELDQLQLNLVLSHACGVGDPIPAPIVRALLAL
                     KINALCKGFSGTRPELALFLQEMLNRSILPVIPCKGSVGASGDLAPLAHMTLVMLGLG
                     EAFYEGERLAGADALARAGLRPVTLKAKEGLALLNGTQVLTAYAAVALQRALNLARLA
                     DIAAAISVESLLNSRTAFDPRIQQARGHREQIRVADNLIRLLSGSEIVESHAHCGKVQ
                     DAYSSRCTPQVHGAVRQALAYAAEVIGTELNASTDNPLIFADENEVLSGGNFHGQPVS
                     LACDALAIAVAQLANISERRLENLMDPVQSGLPPFLAAESGTNSGFMIAQVTAAALVS
                     ENKVLTHPASVDSIPTSANQEDFVSMGTFASRKALEVVQNCETIIGIELLAGCQAMDF
                     RTGLKFGQDPHTFRCALEPGRGTGPAWRLIRSRIGSMPHDRLLHADICTISAMAHENI
                     IVETAETAVGGL"
     gene            complement(12842..13540)
                     /locus_tag="PKM23_04050"
     CDS             complement(12842..13540)
                     /locus_tag="PKM23_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNY90675.1"
                     /translation="MQLGKKELRIIAGLVFLTSFIFIEGISLMSGDFLGSSQRSGNDT
                     ISALLQQQQTMAAIDSQRRTSVNKITGIISRYNHSMSETEKERIANEIYKMTKKYPNL
                     NEDFICATITHETGKTWNTQAKSPMGAMGLMQIMPATAAFLATQEGIDWTSADQVLYD
                     PISNIRLGCRYMSELVGTFNHDGALAAYNGGPKRAALWLASNRNDSTLFKETRSYVPA
                     VNKLYDQFRSEGMM"
     gene            14362..>14984
                     /locus_tag="PKM23_04055"
     CDS             14362..>14984
                     /locus_tag="PKM23_04055"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431901.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HNY90676.1"
                     /translation="MVELCEPDQVHWCDGSQAEYDQICSILIQKGTFRRLNPDLRPNS
                     FLCESDPRDVARVEQSTFICSRQREDAGLTNNWRDPDEMKALLLPIYHGCMRGRTMYV
                     IPYSMGPLGSSISQIGVELTDSGYVAANMRIMTRMGKQVLDVLGDNPFIPCLHSVGKP
                     LRPGEEDVPWPCNPENRYIVHFPEEKMIWSYGSGYGGNALLGKKCFAL"
BASE COUNT         2817 a         4290 c         4999 g         2878 t
ORIGIN      
        1 gggaccgcac cggggtattc agggctggtg cagagcgcct ggacgctcct ggccagactg
       61 cagaaacgct gtgacgtgcc ccgcgtaagg gtctatgaag agtgagcggg acgcagtgat
      121 ttttctttat ccgcgacgct agtcagcatc gcagcgaacg acccggcaaa ggcagccacc
      181 ccatcctgct ggagttcctc cgtcgctccg tccagatcga tccctatctc cgccagggcg
      241 gacagggcag cagagctgag agcggggttg acaggcaggg cggccgcggt gcggccgtgg
      301 tcgagccagg cctgcagggt cgccggcgga atggtgttga cggtgcctgt gccgatgaga
      361 tcgtcgacgt agagggtctc cgaataacgg ggattcttgg tgccggtgct ggcccaaagc
      421 agacgctgca tgcgcgcgcc cttttcggcg agccgctgcc agcgggggga acggtgcagc
      481 tccagaaaaa gatcgtaggt ggcggcggcg ttggccacgg ccattttacc ctgccactgc
      541 gcgaaaccgc gcgcggcgag caagggatcg agtttggtgt caagacggct gacgaagaag
      601 gaggcgacgg aggagagccc gggcagggat cgtccctgcg ccagcagcat ctccagcccg
      661 gtgaaccagg cctcggcgac ctgccggtag tggcgagtgt tgaagatgag ggtcgcgttg
      721 actgaaattc ccgcggccac cacctcgggg atggcgatga tgcccgccgg cgtggcggga
      781 atcttgatca tcaggttggg gcgctccacc gctgcggcca ggcgtttggc ctcgaggatg
      841 gtcttttcag cctcatgggc gagcgcaggg ctcacctcga ggctgacata gccgtccatg
      901 cccgcgctgg attcgtagag ggggcggaag gcgtcggcgg cggcgcggat gtcggcaatg
      961 gccagggcct cgtagagcgg tgcaccggtg aggccgcggg cgctcagggc ggcgatctcg
     1021 gcgtcgtagg ctttgcccgc gctgatcgcc ttctggaaga tggcgggatt tgaggtcaat
     1081 ccgcgcaccc ctgcaccgat caggccggcc agccggcctc cggtgatgaa ttcgcgttcg
     1141 atatagtcga tccagatcga ctggccgcgc gccaaaagct ctgccatttt tatcatttct
     1201 tactccggtt ggggttaatt ggagatgcac tcctaaaaga tcagcagcag atcgacctgc
     1261 cagcgcgttc gggcctgctt gttttggccg gagctgcgct ccagctgccc gaagccgtcc
     1321 ccgatgagcc cgccgagcag ggcaccggcc aggacatccg agagccagtg gcgctcgtgc
     1381 tgcagccgtg aggccccggt gatcagggcc gcggcggcgg cgggcaggcc ggcggcggcc
     1441 ccgtagcgcc gctggagatg ggccgcaacg caggctgacg ccgaggcgtg gccggagggg
     1501 aaggagtcgc ggctgctgcc gtccgggcgc aggcggtgcg tggccagctt gaggaaaccg
     1561 gttgccgcca tgttggcagc ataggcctcg ctgaagctct gccagctgtc aagccgctcc
     1621 cggcggctca tcggccaacc acccaggccg tcggtgagga tgaagagact cccggccaga
     1681 taattggtcc cgaaaccgta atacttgccg aagcgggcga gccctgcggg catgagctga
     1741 ggatgagccg cctgcagatc gcggtcccag gcgctcagcc cggcgaccgc tccggcagcg
     1801 aagagccagc gcgggttgtt gaaagcggct gtcgtcccct gccagatatg gtcgggataa
     1861 ttcccgcccc actccgctgc gggcagcgga gcagcggtca gcaggagaag gcagagcccc
     1921 cggtggaaga aaagtttgcg ccgcctcatg ccagcaggct ttcgttgccg atcagctcat
     1981 ccagacgcct gatcccgagc aggaagaggg tgatgatgaa ttccttgcgc agctgttcga
     2041 tgagctggat cacctcatcg gcggagcgca gggcgggctc gaggaagggc ttggcgatgc
     2101 cgcagaggct ggcgccgagg atcagcgcct tggccatgtc gatgcccgag cggatgcccc
     2161 cggaggcgat gagggttacg cggtcgcgga ggggcgacag ctgcctgagc agatcgggcg
     2221 tagggatgcc ccagtcctga aagagcagcc cggcccggcc gccgtcgctg ctgcggcccg
     2281 gacggtgacg gaaatgctcg atccggctcc aagaggtgcc gccgctgccg gcgacatcga
     2341 tgtagcggat accccgggcc acggccatct cggcatcacg cagggacatg ccggaaccga
     2401 cctccttgag aagaaggggc ctgggcagtg cggcggcaat ggcgccgatc ttgtcggcca
     2461 gtcccctgaa atcagtctcg ccctcgggct ggatggcctc ctgcaaggga ttgagatgga
     2521 gatagagacc atcggcgccc agaatctcaa gggcctgctg gcactcgcgc aggccgaaac
     2581 cgtaattgaa ctggacggcg ccgagattgg cgagcagtac ggttttgggg gcgaaggggc
     2641 ggagggcgaa gctctcccgc gcagcaggat gagtgatcat cacccgctgc gagccgaccg
     2701 ccatggccac gcccgtcgcc tccgctgcct tggccagatt tttgttgatc gcccgcacca
     2761 ggtcgtgatc gcccccggtc atcggcgaga tcatcagggg aaagctgagg ggcttgccaa
     2821 ggaaacgtgt cgaagggtcg atctccttga ggtcgatctc gggcagtgcc cgatgctgga
     2881 ggggccaggc atcaaaatag cttttaccac ggtcggtgct gtcgtcatcg cggacgatgc
     2941 ggatgtggtc atactttctg tggttgattt cttcgctcat gaactcggcc accggattgt
     3001 gattgcaggt ttgtcttgcc taatatagga aaaatatgga taatttccag ccaaatcctc
     3061 ccgggcgtgc agtttgcgtc aggggtggtg ctggatcgcc caggtttcgg aacggatgcc
     3121 cgatgcagcg gaacggtgag cagccggatg gagaggagcg acggtggaga tacacgatct
     3181 tttatatgag agggtgagcg agccggagcc ctttctcttc gagtgctggg agccggaggg
     3241 cgtcgcggtc gtgatcggcc acggccagaa tgcagcacag gaggccgatc tcacggcctg
     3301 ccgccgggac ggggtggtgg tgatccgccg ccgcgggggg ggaggcgccg tcgtgctcat
     3361 gcccggggtg ctctgcctga gctgcgcgtt tttgagcggc aagagcgatt cgccctggtt
     3421 ctattttcag cagatcaatc tcttcatcat cgatctcctg gcacgccgtt tcggcctgga
     3481 ggggctggag ccggctggca tcagcgacat cgccctcgaa ggcagaaaga tcctcggttg
     3541 ttccatgttc aagtcacgcc acctttattt ctaccagggg tcgatgctgg tcaaccccga
     3601 tttgttccgc atctccacct acctcatcca tccctcgcgg gaacccggct accgccgcgg
     3661 ccgcagccac agcgatttcg tcacctccct gtggctgagc ggttatcccc tccccatcaa
     3721 caacctcagg caggccttca gtggagaggc cggggcgctg cgcactctgc tgatctgacc
     3781 ggggtcctgc tcagcgcaag gcgaagcagt agagataatc atagccggcg gcgaaaataa
     3841 gccgacctcc gaacacaacc ggcgaagtgc tgatccgccc cttggcggtg aagctccaga
     3901 gttcccggcc gtcggcgagg ctgagggcat agagcttttt gttcgccgcg ccgatgaaga
     3961 gggtcgagcc gcagatgagg ggagcggcgg ccggcaccgc cccgagatcg aactgccaga
     4021 ccagccggcc atccgtgcgg tcgaggccgt agacgagatt gctggtggag gcgaagagca
     4081 ccagggagtc ggagacggcc gcggcgtaaa ggatcttgcc cgggctttca aatcgccagc
     4141 gctctgcccc ggagacggca tcgatggcac tgaaacggcc gctggttgag ccgatataaa
     4201 agagtccgcc tccggcgacg gccggggcgc gcagggcgcc gccggtctca tactgccaga
     4261 ggctctccag gcggctgttg aaggcatgca agaggccacg gtcatcgccg acggccagaa
     4321 ccgtgtcact catggccggc gctgcgaggc agtcgtggga aaggctggct tcggcgagga
     4381 tggcgccgtc ctcgagggcg atggcgagca gccggccgcg cagggtggcc agatagagcc
     4441 gccctccggc gaccaggggc tcgcccatga ccgggccgat acttctctgc cagcgcatcc
     4501 ggccccgatc cagatcgtag aagagcagat tgtccgccct gagccgcttg accagcacca
     4561 gtccgtcgcc gctccagacg caggtcagtg cggtgttgtt gcggcccagg atccggtcgg
     4621 gtttggctcc gctgcgcaga ttgaaaccct cgatcttgcc gtccttggtt gcgtagagga
     4681 gccactcccc gaaaccgatg aggctgggat tgattccgcc ggtcgcccgc ttcatccaga
     4741 gctgacgcag gggaaagcag ggctcatcgg ggacgacggc gttgcgcgcc ggggatccac
     4801 ccagctgcgg ccagaccagc tcgggagccg ctgcggccgg cttccaggtg aaacggccgc
     4861 agccggccag gaagagcagg gcgaggagga gaactcccag gcgcagggag cggggccgga
     4921 tttgcggccg ccggctgggc agaagggcgc gcatggctaa aagtcccaac caaagaagaa
     4981 ctgggtgaag agcgcctttt cgaaatcgta ctgacggggc tgatagaagc ggccagcatc
     5041 gcggatgaga aattttcgcc ccgtatcaaa gcgcagtacc agcatgccgc ccagctggaa
     5101 gcggacccca aatccggcgc tgccgagcag ctcctccagt ctctcatccc aggcattgcc
     5161 cacatcggcg aagagggcgc cgcggatgct gctgatggct accccgccga aggggaattt
     5221 gagactgaag cggtcgatga agggaaaacg cagttcgttg ctgagcaggg ccaccttggt
     5281 gccccaaagc cgccagaagc ggtagccgcg caggtcccag ctcccgccca tgacgaaggg
     5341 ggtggcctcc ttgccatggt tgatctgtgt ccagagccgc agggcatgag cggcctgggt
     5401 ggagaggcgg aagtagcggc ggtagtcgat gatggcggtg ccgaagcgga cattggcatt
     5461 ggccacatcc acggtagtgc ccagggtgag gttgatgcgg ctgccgtcaa gggggcccgt
     5521 ggggccccag agcgaattgt cctggacgta ggagacaaag ttggagacca gaagggcctt
     5581 gcggcggtag ccgtcggcat accactcctt gtcggaggag cgcacattga ggctggcctc
     5641 gatgcggtcg aatttggaga gggggtagct gagcgccgta tagccgccgt agcgccgttc
     5701 ccagaaccag aggtcgtagc ggttatagta gcggccggcg aagtggaaga ggccgaccgc
     5761 aaagttggtg cggtgcgaga ggtcgacgcg ggagagggcg aagttgaagc tggagagcag
     5821 ctcgctctgc acgcgggcgg tgttgtagag aaggaagaag tactgatagt tgccgagcat
     5881 gtccgagacc accatctggg ccccgcccga ggtgccaaag atgggatcct gggtgatctg
     5941 ggactgggcg atgtcgaggc tgaatttgga gcggtagcga atttccctgc cctcgccttc
     6001 gccgctgatg cgttcatagc tccaggcggt tccggccagg gtgtcgacgg gcgcctcttc
     6061 cgccgcggcc ggctgttgag cagcgcgcag acgggcggcg aggtcgcgct cgaggctgat
     6121 gttgaaggcg aatttctcaa agacggtgaa gaggagatcc ccctgcgggg tccactccgg
     6181 atcataggcc ccggtaatga agcgggtcac ctggcggggg gccgggagat ggaggggggc
     6241 ggcgccggcg ccgggctgca gaccgaggcg ctccgccagg gaaggagcgg cagccgcagg
     6301 cgactccacc acccagatgt tctgcgtgcc gctgcggtcg gagacaaagg cgatgcagct
     6361 gctgtcgggc gaccaggccg gcatctgatc atgcgcttca cctcgggtga gcggggtgat
     6421 gtccccgccg gcgagatcat agataaagag attgtagccg ccttttgctc cgcccgcgcc
     6481 acggtcggag ctgaaggcga tgagccggcc gtcgggcgag aaggcgggat ccttgtcatc
     6541 aaagagatca tccgtgaggg aggtcaattc cccgctctcc cggtcgtaaa gatagaggtc
     6601 gcggcgaccg ctgaaggcga gaccggtaaa gaccaggcgc cggccgtccg gcgaccatga
     6661 aggcgagaag agcgagacca gcttttcaaa gccgtgtcgt ccggtaatct ttttttcggg
     6721 cagctccatg atgtagagga gatcccgttc cccgcttttg gaaatgaagg cgagctcccc
     6781 gcgtccgttg acgtcgatgg cgctgttgag catgtgaaag gactcaaagg cctcgctgcg
     6841 ctcccccgcc accaccactt ccatcggcgg ttccttctcc gcctcggcca gggcggccag
     6901 atagatgttg gcgtagccgg tgcggttgga gatgaagacc acctgcgccg aatccccctc
     6961 ataataggcc ggcttgatgt tgatgccctg acgggtgatc ggggtggtca cgcgcgaggc
     7021 tgcgtcattc tccccgagca ggggatattt ctccttcttg agggcgtagt tccagcgctc
     7081 gtcgaactcg cgcaagtcga tgccgagggt cagcttgatg acctcagaga aatagggcga
     7141 gacccagacg ttctcgatca gctgcagtat cttgtcctcc ccccagccct ggtcgatgaa
     7201 acgcagaaga ctctgcccct ccttgtacat gagaaaggtg ccgttgatct ggtagatgcc
     7261 ggtgacggga cagagatagt tgttgatcac cgcatcgcgg aggaacatct cggcctcgct
     7321 gtcccagccg gccgaccagt attcggccag cccttcgatg aaccagaggg gcaggccggg
     7381 atgggttctc tgtttgtgct ccctgagtac ccggttcact ctggcatggg tgaagacgtg
     7441 gacaagttcg tggcggatga cccgcttgaa ctcggggatg gagccgttgg ccggcacgac
     7501 cacacgcccc ttgagaaact cgaaaaagcc cccgacgcct tcggggatca gataggagat
     7561 ggtgttgctc tcctggaaat gggcatgggt ggcgtaaaag atgaggggga tgcgctcggt
     7621 gaggctgatg ccgaacttgt tctccaggaa gcggtagctc tcctcggcac aggcggcgcc
     7681 gatctcggtc agctcgcgca tctcggggta gtggtagatg tcaaagtgat cggtttgaag
     7741 gatgcgccag tcaaagtggt tatagatcac cttgttgcgc ccgaaatagt attgtgcgcc
     7801 ggcgccgccg gggatgagcc cggcgagcgc ggcgagcagg aggaggctgc gccggaggat
     7861 atggaggaat ctcgcaccgc tcatggcatg ggatcctcaa gtgagacgga ggccggggag
     7921 gagggtggca gtcggcctcc gcattgccgt cgtcgtgctt tcttaaatct ctttctcgta
     7981 gaaacggaag gtcttgtagc gcctggccga gagatcctcg agggcgccga tcatgcgctg
     8041 gttgatctcg aggatgattg aacattcact gtcgacaaag cccagcttga gtccctcctc
     8101 gaaggtcttg agataaaagg caatgtcgat accgcggtgg cggtgctcct tgagcacgcc
     8161 catggccatg gtgcgcaggc gtgtgctctt gcgcacatgc cagaggagct tgagaaggcc
     8221 gaaggggaag aggcggccgt tggccttttg cagggcgggg ttgacatccc ggacgctgag
     8281 ggagaagccg atgcattcac catggtagag ggcgaaaagg gtcacctcgg ggacaatcac
     8341 cattttgagt tccttgacca ggtcttcgac ctggcggtct gtgtagggca catggcccca
     8401 gttctccatc caggcatctg agaagatgcg tgccacctcg cgcacccgct ggtcaaagat
     8461 cttcatgtcg agggggacaa tctccagccc ctcctgatgc atcacccggt cggcgatgcg
     8521 ctgcagcccc ggcctgatct gcgagtccct gctcaccaga aaggcgtacc agtccatcgc
     8581 ctttatcatc ccggcgctgt ccatcaggcg ctcgtaataa gccggattat aggcggtgag
     8641 gatgcagggc atggtatcgt tgccatcaat gagggtgccg ctgatgtcgt acatcgaggg
     8701 gctcatcggc cccatcatgc gttttttccc cttgctgcgc agccattccg cggcagcgtg
     8761 gaccagggcg tctgcaacct gctgatcctc gatgctctcg aaaaagccga aaaaaccggt
     8821 gtggttgtcg tgatagtgtt catactggtg gttgatctgg gcggagatgc ggccgaccgg
     8881 ctcgctgccg cgccaggcca taaagagtgc gatctcgccc aaggtgtaaa aggtcccctt
     8941 cgccggatcg agtattttca tctcatcgcc gatcagctgc gggacccaga gggggtcatg
     9001 acggtagatg cgccagggga atttgatgaa ctgtttgcgt tcccctggtg tggtgatgcg
     9061 ctgtatccgg atgttctcca tggaggacct ttcaggttga ggaaaccgtg gaagaggaaa
     9121 agagaatggg gattatgcaa atatttgttg cattttaata gaaaaattgc tataattaca
     9181 ggctataaaa aaaagagagg aaccatggcg aaccacaaat ctgctgtcaa gagaatccag
     9241 accaatacgc gcgacaacga gcgcaacaag tcctataaaa acacactgcg cacctcggtg
     9301 aagaaggtcc gcgccgtcac cagcaaggaa gagggcgaaa agctgtttcg cagcaccgcc
     9361 tccctgctcg acaaactggc gaaaaagggc attattcaca agaacaaggc cgccaatcag
     9421 aaagccaagc tcgcctccct gattaacaag ctgagttaac cgcgcctccc gatcgcgccg
     9481 tcctctggac cccttttgcc ccgacagcat cctgccgggg ctttttttat ccctccgctt
     9541 caagatcagg ccgtccgcct cctcaggctg tcccccctcg cctgctctcc ggtcctcctg
     9601 caacctagcg gccctgaatc atatagttgg gcgcttcctt ggtgatgatg atgtcatggg
     9661 ggtggctttc gagcagtccg gcctgggtga tgcgcacaaa gcgacccttc tcctgcagat
     9721 cgctgatggt ggctgcaccg ctgtagccca tggccgagcg caggcccccg atcatctgat
     9781 agatcacatc cgccaccttg ccgcgatagg gaacccgccc ctcgatcccc tcgggcacca
     9841 gctttttggc atcctgaatc ccctcctgaa aatagcggtc gccgcggccg ctgcgcatcg
     9901 ccccgagcga gcccatggcg cggtagacct tgtagctgcg cccttcgtag aggatggtct
     9961 cgcccgggct ctcttcggtc cccgccagca tgttgccgag catgacggtg tcggcccctg
    10021 cggcgatggc cttggcgata tcgccggtct gtttgatgcc accgtcggcg atgaggggga
    10081 ctttaagctt gcggcagatc tcggcacagc tcatgatcgc ggagagttgt ggcacgccgg
    10141 tgcccgcaac gatgcgggtg gtgcagatcg agcccggccc gatcccgacc ttgacggcgt
    10201 cagcccccgc cttgatcagt tcctcggccg cctccggggt ggcaatgttg ccggcgatga
    10261 cctcgatctt ggggaactgt tttttcatca gcgccacggt ctggatcacc ccgtgcgaat
    10321 ggccatgggc agtgtcgacg actaggacat cggcgccggc ctcgatcagg gcctccgccc
    10381 gttcctgggt gtcgtgggcg acgccgatag ccgccccgac caaaaggcgg ccgtgatggt
    10441 ccttggaggc gaaggggaag cgtttgcggt tctggatatc cttgaccgtg atcagcccct
    10501 tgagcttgcc ctccttgtcg accaccagca gcttttcgat gcggtgctgc tggagcagct
    10561 tctcggcttc atccagggag gtgccgaccg gagcggtgat gagccgctcc ttgccagtca
    10621 tgagtgatga gacgcgcttg ttgagatcgg tctcgaagcg cagatcgcgg ttggtgagga
    10681 tgccggtgag cttctggccg tcgacgatgg ggatgccgga gatgcggtag cgctgcatca
    10741 gctcgagggc ttcactgagc ttgcggtcgg gggcgagggt gatgggattc atgatcattc
    10801 ccgcctcgga gcgtttgacc ttgtcgacct cggcggcctg ttcctggatg gagcagtttt
    10861 tgtggaggat gccgatgccg ccctcgcggg cgatggcgat ggccaggtcc gccccggtca
    10921 ccgtatccat ggcagcgctg agcaggggga tattgagggt gatcttgcgc gtcagcctgg
    10981 tgcggacgtc ggtttccttg gggaggacat ccgatcggtt cggcaccagc aggacgtcat
    11041 caaaggtata gccttcaccg atgatcttgt tcatcgctct cgctctccag aatacttaca
    11101 gtttttgagg ccgtcagcac cggccgttgt ttcacttttt acagcccacc caccgccgtc
    11161 tcggcggttt cgacgatgat gttttcgtgc gccatggcgc tgatggtgca aatgtcggca
    11221 tgcagcagcc ggtcatgcgg catcgagccg atgcggctgc ggatgagacg ccaggccggt
    11281 ccggtgccac ggccgggctc gagggcgcag cggaaggtgt gggggtcctg gccgaatttg
    11341 aggccggtgc ggaaatccat ggcctggcag ccggcgagca gttcgatgcc gatgatggtc
    11401 tcacagttct gcaccacctc cagggccttg cggctggcga aggtccccat gctgacaaag
    11461 tcctcctggt tggcggaggt cgggatcgaa tcgaccgaag cgggatgggt gagcaccttg
    11521 ttctcggaga ccagggcggc ggcggtgacc tgggcgatca taaagccgga gttggtgccg
    11581 ctctcggcgg cgagaaaggg ggggaggccg ctctggaccg ggtccatcag gttctcaagg
    11641 cgccgttccg agatattggc gagctgggcg acggcgatgg cgagggcgtc gcaggccagg
    11701 gagaccggct ggccgtgaaa attgccgccc gagaggacct cgttttcatc ggcgaagatg
    11761 aggggattgt cggtcgaggc gttgagttcg gtgccgatca cctccgcggc gtaagcaagg
    11821 gcctggcgca ccgccccgtg cacctgcggg gtgcagcgcg aggagtaggc gtcctggacc
    11881 ttgccgcagt gggcatggga ctcgacgatc tcgctgccgc tgagcagtcg gatgagatta
    11941 tccgccacac ggatttgctc acggtggccg cgggcttgct ggatgcgcgg atcaaaggca
    12001 gtgcggctgt tgagcaggct ctcgacgctg atggctgcgg cgatgtcggc cagccgggcc
    12061 agattgaggg cgcgctggag ggcgaccgcg gcataggcgg tcagaacctg ggtgccgttg
    12121 agcaacgcca gtccctcctt ggccttgagg gtcaccgggc gcagaccggc ccgggcgagg
    12181 gcatcagctc cggcgaggcg ctcgccctca taaaaggcct caccaaggcc gagcatgacc
    12241 agggtcatgt gggcgagggg ggcgaggtcg cccgaggccc cgaccgaacc cttgcagggg
    12301 atgaccggga ggatgctgcg gttgagcatc tcctggagga aaagggccag ctccggccgg
    12361 gtgccggaaa agcccttgca cagggcgttg atcttgaggg cgagcagggc gcgcacgatg
    12421 ggcgcgggaa tggggtcccc gactccacag gcatggctga gcaccaggtt aagctggagc
    12481 tggtcgagtt cgctgtcggg gatgtggata ttggccagct tgccgaagcc ggtgttgaca
    12541 ccgtaaacca cccggccttc agcgatgatc ccctcgatgc aggcgcggga ggcggtcatg
    12601 cgcgccatgg ctgcggtcgt gatcgcgacc aggcggccgg agacgacggc gtgaaggtcg
    12661 gcgagggtga gggtgtgtcc gtcgaggagg atgggctgca tggtgctttc tccctgaaaa
    12721 atgcgctaaa gtttaaaaaa aaatcgcgag ataaacaact tgtttttcag ccggaaaggg
    12781 ataaaaaaaa ggcggcgccg gtcgggcgcc gcctggtggt tggcaggtgt gtgcggcagg
    12841 cttacatcat gccttcgctg cggaactggt cgtagagctt gtttaccgcc ggcacatagg
    12901 agcgggtctc cttgaagagg gtgctgtcgt tgcggtttga ggccagccac agggcggccc
    12961 gtttgggccc gccgttgtag gcggcaaggg cgccgtcgtg gttgaaggtg cccaccagtt
    13021 cgctcatgta gcggcagccc agacggatgt tgctgatcgg atcgtagagg acctgatcgg
    13081 cgctggtcca gtcgatcccc tcctgggtag ccagaaaggc ggcagtggct ggcatgatct
    13141 gcatcagccc catggcaccc atgggcgact tggcctgggt gttccaggtc ttgccggtct
    13201 catgtgtgat ggtggcgcag atgaaatcct cattcaggtt cgggtacttt ttggtcattt
    13261 tgtagatttc attggcgatg cgctcttttt cggtctcgga catggagtgg ttataacgcg
    13321 agatgatgcc cgtgattttg ttgacactgg tgcgtcgttg gctgtcgatg gcagccatgg
    13381 tttgctgctg ctgcaggagc gcgctgatgg tgtcgttccc gctgcgctgg gaagagccca
    13441 gaaaatcgcc cgacatcagg ctgataccct cgatgaatat aaagctggtc agaaaaacca
    13501 atccggcgat tatcctcagc tcttttttac cgagctgcat cttgccctcc aataatcgtt
    13561 gattccgatc tttagaggcc ttcacggttg cattgagctt cgccactttc tgcttgagct
    13621 gttctttcga aactattttg ttgagctgtg ctttcatttt ctcttctttg ttggtccgac
    13681 ttttcctccg ggatgcgcct gctgtcgtaa ttttgtgtcc atttcaggca ccgcggtgtg
    13741 aaatggttac tagagtatgc gcatcgagga gatttaacag aatggtccgg ggttctattc
    13801 cccgaaattc cccttctcac caatactatt agcaaaccat gtgccaaaac aggcgccgat
    13861 gagggtggtt tggtgaaaag gagcctataa tataaagaaa atattaaaaa tagtcaactc
    13921 ctatctgctc aaattacctt tttgtaacca ggcctgcagg gggcggtctg aacgagcagg
    13981 gggagaaagt ggattgaaat gaggcagcct cgcacggctc cttccggggg gtgagtcatt
    14041 tcaggccaat ttctccgcat gaaaagtgcg ccgcatcaca tcatcgggcc gggatgccgc
    14101 tacttcttaa atgtaataaa atcaattaat atagccgacg agtgccatct ccggccggag
    14161 gaagggcgcc ttttgttccc ggcggggaag cccccccgcc ttgtgtctcg ctctgagaca
    14221 atagaggccg cctggccggg gcggccatta gctcttgact ctcatgcaaa aactgagtat
    14281 gttatcacgc tgaataaacc atacacaaac tgaggagcgg aaatgacctc taatattcgg
    14341 ttaaaacaat gggttaagga aatggtggaa ctctgcgaac ccgaccaggt gcactggtgt
    14401 gacggttcgc aggcggaata tgatcagatc tgttcaattt tgatacagaa gggcactttt
    14461 cgccgcctca atcccgatct gcggcccaac tctttcctgt gcgagtccga tccccgcgat
    14521 gtggcgcggg tcgaacaatc cacctttatc tgctcacgcc agcgcgagga tgccggtctg
    14581 accaacaact ggcgcgatcc ggatgagatg aaggcactcc tgctccccat ctatcacggc
    14641 tgcatgcgcg ggcgcaccat gtacgtcatt ccctacagca tggggcccct gggctcgtcc
    14701 atcagccaga tcggcgttga acttaccgac tccgggtatg tggcggccaa catgcgcatc
    14761 atgacacgca tgggcaaaca ggtgctggat gtcctgggcg acaatccctt catcccctgc
    14821 ctgcattcgg tgggcaaacc cttgcggccc ggtgaagagg atgtgccctg gccctgcaac
    14881 ccggagaacc gctatatcgt ccactttccc gaagagaaga tgatctggtc ttacggcagc
    14941 ggctacggcg gcaacgccct gctcggcaaa aagtgcttcg ccct
//
LOCUS       DAOPWU010000043        14671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4183_length_14671_cov_4.405583, whole genome shotgun sequence.
ACCESSION   DAOPWU010000043 DAOPWU010000000
VERSION     DAOPWU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14671
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4183_length_14671_cov_4.405583"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PKM23_04060"
     CDS             complement(<1..880)
                     /locus_tag="PKM23_04060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNY90677.1"
                     /translation="MRLPALIFVLISLLAAPLLTGWIVRVKALFAGRRGAPLLQPYRD
                     LFRLLRKGAVYSRSTSPLFWLAPMATLAATAAALCLLPLPGYTALISFPGDLVLFAYL
                     FALGRFFTILAALDTASAFEGMGASREAWYAIFAELILFLALANLARFTNSLSLSQIF
                     ESRAVSINYADSPALALLVVSLFVALLTENCRIPVDDPTTHLELTMIHEVMVLDHSGP
                     DFAFILYGAALKLWIWAMFITLVIISSFPSPNSLRPLIAAGVLFIIATAIGIVESVMA
                     RLRLERVPQMLMGAGAL"
     gene            complement(877..2886)
                     /locus_tag="PKM23_04065"
     CDS             complement(877..2886)
                     /locus_tag="PKM23_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNY90678.1"
                     /translation="MNPLLSCFFIALLILLCGGAAAWLLHRREVLARRLAAAGVIAAG
                     VLGLVPAVGVLASGESLFFEQAASIPALSFSLMIDPLAAFFLLIIFLISALAALYGAG
                     YLQGEGSAAARGRSFFFFNLLVLGMALVAAAANGMLFIIAWEIMSMAAFFLVTFEDER
                     PQTRSAGLIYLIAAHIGVVLLFFMFWLLAGASAGSLDFNQFAGAALAGPKLRSLIFLL
                     GLAGFGAKAGFLPLHVWLPEAHPAAPSHVSALMSGVMIKMGLYGILRTLTWLGTPPLW
                     WGALLVVIGLVSGLLGVLYALAQHDLKRLLAYHSVENIGIMAIGLGLGLLGFHLGEQT
                     LAVFGFAGCLLHILNHSIFKSLLFMGAGAVKQQCHTLEIDRLGGLQKRMPLTAAAFII
                     GAAAISGLPLLNGFVSEFMIYFGAFDAVTSMAKGATALPLLSLLGLAAIGALAVACFT
                     KASGVVFLGEARTPAGEGAREVSAVMLLPMLLLAGLCIAAGVAPLLLLPLLQPVIAAT
                     AQLPAAVIADELARMVRPFIAVTGIAAGSALLFALLALLRRRILARRGISRAVTWDCG
                     YSRPTARMQYTASSFVQPLTDQNRWILGSRVEEEPPQDYFPDKGSLHSHTPDLFLEKL
                     WQPLLAAIAWLFHQLQWMQRGRISLYILYIAVVLLALLFWFGGLR"
     gene            complement(2883..3563)
                     /locus_tag="PKM23_04070"
     CDS             complement(2883..3563)
                     /locus_tag="PKM23_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNY90679.1"
                     /translation="MQLQIRDICQLLNVSERTVYRWIKSGAIPHYKLNEQYRFNRVEI
                     LEWATAQKMTVSPEIFAEPESGDLPALSAAIEAGGIHYRVSGRDKESVLRSIVRQMRL
                     PEGVDVDFLLQVLLARESLGSTAIGDGIAIPHPRSPIVLHVSRPIISLSFLETPIDFE
                     APDGKPVGIIFTIVSPTIRAHLHLLSRLSYALKDDSWRRILAAPGVREEILDLLKRIE
                     PGFGRAAE"
     gene            complement(3667..4875)
                     /locus_tag="PKM23_04075"
     CDS             complement(3667..4875)
                     /locus_tag="PKM23_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90680.1"
                     /translation="MQLRIEELTQQYIRLWHETPVTLPDLGVVYSCDQKRELAREAAL
                     QLDRLEISRQQGARDREKVQNALEESRAELEGLCDRAGLYFDRAFTDGFGQATREFLS
                     RIKAFDPELPPENIWQAMRNLWIINSLQVMMGKPMACPAPSFAYSMLYPYSDNVIDDP
                     DLCLTEKWATNDRFRRWLEGEEVLPLTATEEKIRALVQIIEAGFSRQACPGLFQSLLG
                     IFNAQIKSILQQKQHRSGREADLLGISVEKGGSSVLADGYLIQGALDAAWEDFTFGYG
                     VFLQFADDLQDLDEDLKNGHQTLFTRAAENGPLDGLANRLFHFMTRVIDLHLTAPAQQ
                     RCRALIHKNCFFMVQEAICKTAAHYSPAYIRTIERHFPLSLPYYRQVKLRLKKMLLET
                     ERSAGALVMA"
     gene            5192..7522
                     /locus_tag="PKM23_04080"
     CDS             5192..7522
                     /locus_tag="PKM23_04080"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNY90681.1"
                     /translation="MIRKLLFFALLLPALLTGQSDDSWKVFDDAEPARIDITIDPAAL
                     DWILTHAESDSEHYARFHFRNRYIDESLDSIGFRLRGNTSREAAKKSFKVSFNTFIQG
                     REFHGLDKLNLNGEHNDPSIIRSKLCFDLYQDIGRAASRASHLRLYINGRYYGLYISV
                     EHVDDEFLSKRFADDSGNLWKCLYPADLRYLGNDPRFYKSLASGGRPAYELTTNEERG
                     DFGALARLIRVLNLTPAAALPDSLEKLLDVPGVLQYFAMNVLVGSWDDYRALMNNYYL
                     YCEPSSGRFTLIPYDYDNTFGIDWFNTDWSAADPYNFPKVANGDRPLAERLLIVPAWR
                     DLYTHFLDFYLNSVFRLPRWENRIDRLMTSITAAAEADSFRTRDYGFTMADFSGSYTA
                     GDYHKQHVKFGLKQFINLRSASLPGQLRWHRAAPVVYRIDIDPRHPATGDSLHVAAAC
                     FAAAGLERVTLLFTPDGSLATTSWPMQHRPVAKTKKVEEADRWIAVLPPQATGSAGSF
                     RIEAVDSLAQIRTYPLTGPIEVKTAGGYTGLLRLNEFMADNSTAVADEAGDHDDWVEI
                     YNSGSEPAALAGLWLTDKRDRLAKWRFPSTTPELPPGAWLVVWCDEEQEEGSLHTNFK
                     LSAGGEFLALVDRDGITLLDSLTFGPQTRDLSHGRYPDGLGTWQTTAPTAGSANRTAS
                     HVRQRAQATDFTLQAWPNPFNAAAQLHFTLPQAGRVTLQLFNTLGRQVQILFDGPLES
                     GKHVVQVDAEELPSGILFCCLRSGGLCKTIKLLVVK"
     gene            complement(7733..8746)
                     /locus_tag="PKM23_04085"
     CDS             complement(7733..8746)
                     /locus_tag="PKM23_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90682.1"
                     /translation="MKKNSKTTLSALLLILWIAGCREIKTTTTVYGDGRIERKIAIEG
                     DADKLHESAFPLPIDSTWFRQCTLQAQDSTQTVTTWSRQFSGIAELQQCYKSVSPPAF
                     LIKVDIELEQKSRWFYTYWQYRETYHAYNIHTLMPVSDYLSADELLFWINQPDSSDEV
                     EEKMEQWLMDNLFAEFYQTLQDEVKQLNDPRLDVRQLDAHKSELYYVLMDSSSHADEA
                     DEILAVCQKIYGSDVLSAARVPIDSSLKSLEKHVEFETDLSSNTYANTVFLPGQLLAS
                     NGHIGEQGQVTWNLTSRNFQFEDFSMWAQSRKANTGLTILSAIMAVSLTAGSIIALVK
                     SRK"
     gene            complement(8733..8957)
                     /locus_tag="PKM23_04090"
     CDS             complement(8733..8957)
                     /locus_tag="PKM23_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90683.1"
                     /translation="MGLIGTATMPDICSAALAREREGREKKKTDPTQAAHEILQILWQ
                     DGPDMVHAVNETLDYKIIFLLNGEELNEKE"
     gene            9281..9883
                     /locus_tag="PKM23_04095"
     CDS             9281..9883
                     /locus_tag="PKM23_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNY90684.1"
                     /translation="MARQQDILTAARELFLRQGYHETTLEEIARHAEFGKGTIYNYFD
                     SKEDLFLAIFDQLIGELDTIARTTLSEGEGEGRQRLAVYAGTMVHYSRDNAKLIALVM
                     HQIHQISQEKRKPFLARYAAVMQKVWQIIGLTIRSEQEQHGACRYDPMALAILFDGMV
                     RTYNMTRFGPLHSMPEEEIEQAADMITTIFFNGIGKPNQG"
     gene            9937..11253
                     /locus_tag="PKM23_04100"
     CDS             9937..11253
                     /locus_tag="PKM23_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNY90685.1"
                     /translation="MSRSQAFQAAPLPAARDTLVLSIEQAIAMALHQNRDLMIAGEER
                     VKANAQVGEAKSGAFPQLTASGQYMRYIKKQVMFLPPGNAINPTDKTLLFEIGSNNAY
                     SGAVQLSQALFSRKVGVALQIAKTYRQLAAESYSAAQQAVVRDTRKAYYQILLAQKLV
                     EANRQGLEVIQANYTNIQAQYRVGAAAEYDLIRAEVQLANTEPLVISAENGYQLAVNA
                     LKNLLAIPLDQPVVIAGELSYEELPAEVAKQAEAQALAVNPAIKQLSLQEQMLDRNIS
                     IEKAGNFPTLYAVGSYIWQSQDNTFKFGNYNWANTVNAGVSLSYPLFDGFRTRNRVQQ
                     AKVERNKVRYNRLKMEEGLRVQIQAGALRMAEARQRMSGQEKTIEQAQKAVRIAQTRF
                     KSGVGTQLELLDAQVAMTRSQTNYAQALYDYLVSKTEWEFATGTSR"
     gene            11312..12358
                     /locus_tag="PKM23_04105"
     CDS             11312..12358
                     /locus_tag="PKM23_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNY90686.1"
                     /translation="MFGLILISAFSALALMGGCGRAEKAAVANPVNRGVEVVPVETAR
                     VERQSLAVTKSYTGTLEGEAQANIVAKISERITAINGSVGQSVQKGRTVIALDKSGTT
                     SQYYQAEANYKNAEKNLERMKALLAEGAISQQLLDGTQTAFDIARANFEAARSAVELV
                     SPISGVVTALNVNLGDLAQPGAVLATVADIHRMKVIFSINEADVMQLAAGQSVEIWSE
                     GGPEGRAQGKIVQISRSADVRSRSFEIKALFPNTADQWYRPGMFCKVAVTISPRNNAL
                     VVPAAAILSDGLTSRVYKVVGGRAIPQPVRTGISDGARVEILEGVTEQETVVTTGATN
                     LRDSSLVAVTPALQ"
     gene            12395..>14671
                     /locus_tag="PKM23_04110"
     CDS             12395..>14671
                     /locus_tag="PKM23_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNY90687.1"
                     /translation="MKLADVSIERPVFATMMILALLVLGLFSYMKLNIDQFPDIDFPY
                     VTATTVLPGAGPEQIETVVTKKIEDAVNTIGGLDHIQSISQENVSIVIIQFKLEVDGK
                     QAAQEVREKIAAIRANLPDDIEDPVIQRIDPASTPIYTLALSGSLGDKELTTFAKDVV
                     RKRLENVPGVGRVDLVGGAEREIEIQVDAARLQAYNLSIQEVVQAIAAANVEVPAGNL
                     IQGERQILLRTTGKYTDVADFDKVIVASPGGKPVYLSEIATVVDGIKERTSLSRVDGN
                     AAVVLSILKQSGSNTVRVADGVTAQIGAIRKELPPHIQLATVRDNSTFIRDSVRDVLF
                     DIIYGGLLAVIVVFLFLANHRPTLISAIALPTSIIASFIIMFALKFTLNILTLLALSL
                     AVGLLIDDAIVVIENIYRHLMKGESPMEAARKATGEIGLAVMATTFTIVAVFVPVAFM
                     PGIAGRFFYQFGITVSAAVLVSLFVAFTLTPMLSSRWLREEDEELTNTGSPLRRGLYW
                     FNHFFEVLAEKYKKAIDWSLHHRKTMVFGAMATFFFSLFLMRFLATAFMPSFDMSQMT
                     VTVTAAPGSSLEQTSRICNKIEDALHQRPEVTMVLTTIGAGTDPVTKGSVFANLVKKD
                     QRDKGVEQIIGELRSELQTIPGANIGFTVQQGMGGMQKPMIMSVRGEDLNVLKRLADQ
                     VERIVRTTPGAVDVENSLETSKPEVRIRIDREKASDLGANVALIASTARAMVDGYVAS
                     TFQEGDEQFDVRVRLNKED"
BASE COUNT         3116 a         4405 c         4419 g         2731 t
ORIGIN      
        1 caagggcacc ggcgcccatc agcatctgcg gcacccgctc aagccgcagg cgcgccatca
       61 ccgattcaac gatgccgatg gcggtggcta tgatgaaaag aacgcccgcc gcgatcagcg
      121 gccgcagcga gttgggcgaa ggaaaggagg agatgatcac cagggtgatg aacatcgccc
      181 agatccacag tttgagcgcc gcgccgtaga ggatgaatgc gaaatcgggg ccgctgtgat
      241 ccagcaccat cacctcgtgg atcatggtga gttcgagatg ggtggtggga tcgtcgacgg
      301 ggatgcggca gttctcggtc agcagggcga cgaagaggct gaccaccagc agggccaggg
      361 ccggactgtc ggcatagttg atggagacgg cacggctctc gaaaatctgc gaaaggctga
      421 gcgaattggt gaagcgggcc agattcgcca gggcgagaaa aaggatcagt tcggcgaaaa
      481 tggcgtacca ggcctcgcgg ctggccccca tcccctcaaa ggccgaggcg gtatcgagcg
      541 ccgccaggat ggtgaaaaag cgcccgaggg cgaaaagata ggcgaaaagg acgagatcgc
      601 ccggaaagga gatcagggcg gtatagccgg gcaagggcag caggcagagg gcggcggccg
      661 tcgcggccag ggtcgccatc ggcgccagcc aaaagagggg gctggtgctg cggctgtaaa
      721 ccgccccctt gcgcagcagc cggaagagat cgcggtaggg ctgaagcagg ggcgcgccgc
      781 ggcgcccggc gaagagggcc ttgacgcgga cgatccagcc ggtcagcagc ggggcggcca
      841 gcagggagat caggacaaag atcagggcgg gcagcctcat cgcagacctc cgaaccagaa
      901 gagcagggcg agcaggacca cggcaatgta gaggatatag agcgaaatac ggccgcgctg
      961 catccactgc agctggtgga aaagccaggc gatcgccgcc agcagcggct gccacagctt
     1021 ttcgagaaag aggtccgggg tgtgcgaatg cagggagcct ttgtccggga aataatcctg
     1081 cggcggctct tcctccaccc tgctgcccag gatccagcgg ttctggtcgg tcaggggctg
     1141 gacgaacgag gaggcggtgt actgcatgcg cgcggtggga cggctgtagc cgcagtccca
     1201 ggtgacggcg cggctgatgc cccttctggc gaggatccgg cgtcgcagca gtgcgagcag
     1261 ggcgaagagc agcgcactgc cggcagcgat ccccgtcacc gcgatgaagg gcctgaccat
     1321 cctggccagc tcatcggcga tgactgcagc cggcagttgg gccgtcgcag cgatcaccgg
     1381 ctggagcagg ggcagaagca gcaggggtgc aacacccgcc gctatacaga ggccggcgag
     1441 caggagcatc ggcagcagca tgacagcgga gacctcccgg gcgccctctc cggccggcgt
     1501 acgcgcctcg ccgagaaaga ccacgcccga ggccttggtg aaacaggcca cggccagggc
     1561 gccgatcgcc gccagtccga gcaggctgag caggggcagg gcggttgccc ccttggccat
     1621 ggaggtgaca gcgtcaaagg ccccgaaata gatcatgaat tcgctgacaa agccgttgag
     1681 gagagggagc ccggagatgg cagcggcgcc gatgataaag gcggcggctg tgagcggcat
     1741 gcgcttctgc agcccgccga ggcggtcgat ttccagggtg tggcactgct gcttcaccgc
     1801 tccggccccc atgaaaagca ggctcttgaa gatggagtgg ttgaggatgt gcagcaggca
     1861 gccggcgaat ccgaaaaccg cgagggtctg ctcccccaga tggaagccga gcagccccag
     1921 gcccaggccg atggccatga tgccgatatt ctcgacactg tgataggcga gcagccgctt
     1981 gaggtcgtgc tgggcgagag cgtagagcac cccgagcagg ccgctgacca gtccgatgac
     2041 caccagcaag gcgccccacc acagcggggg cgttcccagc caggtgaggg tgcgcaggat
     2101 gccgtagagg cccatcttga tcatcacccc ggacatcagg gccgaaacat ggctcggggc
     2161 ggccggatgg gcctcgggca gccagacgtg gagggggagg aatccggcct tggcgccgaa
     2221 tccggccagg ccgagcagaa agatcaggct cctgagcttc ggtccggcca gtgctgcccc
     2281 ggcgaattgg ttgaaatcga gcgaacccgc gctcgccccg gccagcagcc agaacatgaa
     2341 aaagagcagg acgacgccga tgtgggccgc gatcaggtag atcaggccgg cgcttctagt
     2401 ctgcggccgt tcatcctcaa aggtgacgag gaaaaaggcc gccatcgaca tgatctccca
     2461 ggcgatgatg aagagcatgc cgttggccgc ggccgcgacc agggccatgc cgaggacgag
     2521 gaggttgaaa aagaaaaaag agcgacctcg cgcggcagcg ctgccctcgc cctgaagata
     2581 accggcgccg taaagggcgg ccagggcgga gatgagaaag atgatgagca ggaaaaaggc
     2641 cgccagaggg tcgatcatca gggagaagga gagcgccggg atcgaggccg cctgctcaaa
     2701 gaacaggctc tcccctgagg cgagcacgcc cacggccggg accaggccca ataccccggc
     2761 ggcgatcaca ccggcggccg cgagtcggcg cgccaggacc tcccggcggt gcagcagcca
     2821 ggcggccgcg ccgccgcaga gcagaatcag cagggcgatg aaaaaacagc tcagcagggg
     2881 attcattcgg ccgccctgcc aaagcccggt tcgatccgct tcaggaggtc gaggatctcc
     2941 tcgcgcacac ccggcgcggc caggatccgc cgccaggagt catccttgag ggcgtaggag
     3001 aggcgcgaga gcagatgcag gtgggcgcgg atggtcggac tgacgatggt gaagatgatg
     3061 ccgaccggct tgccatcggg ggcctcgaaa tcgatcgggg tctcgagaaa ggagagcgag
     3121 atgatcggcc gtgagacgtg caggacaatg ggactgcggg gatggggaat ggcgatgccg
     3181 tcgccgatcg ccgtcgagcc cagggattcg cgcgccagca gcacctgaag gaggaaatcg
     3241 acatccactc cctccggcag gcgcatctgc cgcacgatcg agcgcagcac gctctccttg
     3301 tccctgccgc tgacgcggta atggatcccc cccgcctcga tggcggcgga aagtgccggc
     3361 aggtcgccgc tctcgggctc ggcaaagatc tccggcgaga ccgtcatctt ttgtgccgtc
     3421 gcccactcga gaatctcgac gcggttaaag cggtactgct cgttgagctt gtaatgggga
     3481 atggccccgc tcttgatcca gcggtagacc gtgcgctcgg aaacattgag gagttggcag
     3541 atatcccgga tttgcagctg cattcttcac cctgatctgt caatgatgtc gtgactttgt
     3601 caaattaggg caaaatatag taaatgtcag ggaaaatggc aaacaaaaaa ggagggcggc
     3661 ggcccgtcag gccattacca acgctccggc gctccgctct gtctcgagca gcatcttttt
     3721 cagccgcagc ttgacctggc ggtagtacgg cagggagagg gggaaatggc gttcgattgt
     3781 gcggatatag gcgggcgagt agtgcgccgc ggttttgcag atcgcttcct gcaccatgaa
     3841 gaagcagttc ttatggataa gcgctcggca gcgctgctgg gcaggagcgg tcaggtggag
     3901 gtcgatcacc ctggtcatga aatgaaagag ccggttggcc agaccgtcaa ggggaccgtt
     3961 ttcggcggcc cgggtgaaga gggtctggtg gccgtttttc agatcctcat cgagatcctg
     4021 caggtcgtcg gcgaactgga gaaagacgcc gtaaccgaaa gtaaagtcct cccacgccgc
     4081 atccagtgcg ccctgtatga gatagccgtc cgccagcacc gagctccccc ctttttcgac
     4141 gctgataccg agaaggtccg cctcccggcc ggagcgatgc tgcttctgct gcaagatgct
     4201 tttgatctgg gcgttgaaaa tccccagcag gctttgaaaa agcccgggac aggcctggcg
     4261 cgagaagccg gcctctataa tctgcaccag ggcacggatt ttctcctcgg tggcggtgag
     4321 gggaaggacc tcctccccct ccagccagcg ccggaagcgg tcgttggtgg cccatttctc
     4381 cgtcaggcag agatccggat catcgatgac attgtcggag tagggataga gcatactgta
     4441 ggcaaaggac ggcgccgggc aggccatcgg cttgcccatc atcacctgca gggagttgat
     4501 gatccagagg ttacgcatgg cctgccagat attctccggc gggagttcag ggtcaaacgc
     4561 cttgatacgg ctgaggaatt cccgggtggc ctggccaaaa ccgtcggtga aagcgcggtc
     4621 gaaataaagt ccggccctgt cgcagagccc ttccagctcc gcgcgggact cctctaaggc
     4681 attctgtact ttctcccggt cccgggcgcc ttgctgccgg cttatttcaa ggcgatcgag
     4741 ttgcagagcg gcctcccggg ccagctcccg cttctggtcg cagctgtaga cgacgcccaa
     4801 atcgggaagg gtcaccgggg tttcatgcca gagccggatg tattgctgcg tcagctcttc
     4861 aatcctgagc tgcatcccgc ccattctccc ggacaagaga ccggctaaag gagattcccg
     4921 ttctgatgct gcgttgtgca tgactgccct ttacgctttc cacaataaca ggatgtaaca
     4981 gacagctttg acctgaagag agcgcctcct ttccatgaac atggaggcgc ttgtctatat
     5041 cgtatactgc aaaggaccag gaatgtttca gccacaggcc gtcccgcccg gaattcgctc
     5101 ttgattcttg cgctgcaaat ggctaccttg cgcccgggag gaaatcgatt cacctgcttg
     5161 ggcactacag ataatccgaa agaaggcctc tttgatcaga aaactgctct tttttgcatt
     5221 gctgctgccg gccctgctca ccggccagag cgacgacagc tggaaagtgt tcgacgatgc
     5281 cgagccggcg cgcatcgaca tcaccattga ccccgccgca ctcgactgga tcctcaccca
     5341 tgccgagagc gacagcgagc actatgcccg ctttcatttc cgcaaccgct acatcgacga
     5401 aagcctcgac tcgatcggct tccggctgcg cggcaacacc tcgcgggaag cggccaagaa
     5461 atccttcaag gtatcgttca acaccttcat tcaggggcgg gaatttcacg gcctcgacaa
     5521 gctcaacctc aacggtgagc acaacgaccc ttccatcatc cgcagcaaac tctgcttcga
     5581 tctctatcag gatatcggcc gggcagcctc ccgcgccagc cacctccggc tctacatcaa
     5641 cggccgctat tacggactct acatctcggt cgagcatgtc gatgacgaat tcctgagcaa
     5701 gcgtttcgcc gacgacagcg gcaacctctg gaaatgcctc tatccagccg atctgcgcta
     5761 tctgggaaac gatccgcgct tttacaaaag cctggccagc ggcggccgcc cggcctacga
     5821 gctgaccacc aacgaggaac gcggcgattt cggggcgctc gctcggctta tccgtgtcct
     5881 caacctgact ccggctgccg cgctgcccga ctccctcgaa aagctcctgg atgtgccggg
     5941 cgttctccag tacttcgcca tgaacgtcct ggtcggcagt tgggatgact atcgcgccct
     6001 gatgaacaac tattacctct actgcgagcc ctcctcaggc cgcttcaccc tgatccccta
     6061 tgattatgac aacaccttcg gcatcgactg gttcaacacc gactggagcg cggccgatcc
     6121 ctacaacttt cccaaggtag cgaacggcga ccgccccctg gccgagcggc tgctgatcgt
     6181 gccggcctgg cgcgacctct acacccattt cctggacttt tatctgaatt cggttttcag
     6241 gctgccgcgc tgggagaacc gcatcgaccg gctcatgacc tcgatcacag cggctgccga
     6301 agccgattcc ttccgcaccc gcgattacgg ctttaccatg gccgattttt cgggctccta
     6361 cacggcgggc gactatcata aacagcatgt caaattcggc ctcaagcagt tcatcaacct
     6421 ccgcagcgcc agcctgcccg gccagctgcg ctggcaccgc gcagcgcctg tggtctaccg
     6481 catcgatatc gatccccgcc atcccgcaac cggcgactcg ctccatgtcg ccgccgcctg
     6541 ctttgctgcc gccgggctcg agcgggttac cctcctcttc acgccggatg gctccctggc
     6601 aaccacctca tggcccatgc agcaccgacc tgtagcgaag acgaaaaagg tcgaggaagc
     6661 ggaccgctgg atcgccgtgc tcccgcccca agccaccggc tcggccggga gcttccgcat
     6721 cgaagccgtg gactcgctgg cacagatccg cacctatccc ctgaccgggc cgatcgaggt
     6781 gaaaaccgcc ggcggctaca ccggactcct gcgcctcaat gagttcatgg ccgacaacag
     6841 caccgctgtc gccgatgaag cgggcgacca cgatgactgg gtcgagatct acaactcggg
     6901 cagcgagccg gccgctctcg ccggactttg gctgaccgac aaacgcgacc gcctcgccaa
     6961 atggcgtttt ccgtcaacaa ctccggagct gccccccggc gcctggctgg tcgtctggtg
     7021 cgacgaagag caggaagagg gcagcctgca taccaatttc aagctcagcg ccggcggcga
     7081 gttcctcgcc ctggtcgatc gcgatggcat caccctcctg gattcgctca ccttcggccc
     7141 ccagaccagg gatctcagcc atggccgcta tcccgatggc ctaggcacct ggcaaaccac
     7201 agccccgaca gcgggctcgg ccaaccggac ggccagccat gtccggcagc gcgcccaggc
     7261 gacggatttc actctgcagg cctggcccaa tcccttcaat gcggctgcgc agctacattt
     7321 cactctgccc caagccggcc gggtcaccct gcagctcttc aacacactgg gccggcaggt
     7381 acagatcctc ttcgatgggc cgctggagag cgggaagcat gtggtacagg tggatgcgga
     7441 ggagctgccg agcgggattc tcttctgctg cctgagatcg ggcgggctgt gcaaaacgat
     7501 caagctgctg gtggtgaaat agcccctggt gcgcctgctc caatagtgag actttttttc
     7561 tgaaacattt ctgccaggag gcaggtagac acgatttgca tgggatgaaa caggccaaac
     7621 cttaacaagg aggaatgaac caatacgaag ctggctgaca ctcatcatgc gcatcatggc
     7681 gcgcagccag gtctccggag cgatgtagcc aacggcgccg ctgctcagga agtcattttc
     7741 tggatttaac aagcgcgatg atcgaacccg ctgtgagaga gacggccatg atggcggaca
     7801 agatcgtcag accagtattg gcttttcggc tctgcgccca catggaaaaa tcctcgaatt
     7861 gaaaattgcg cgaggtgaga ttccaggtaa cctggccttg ctcgccgata tggccgttgc
     7921 tggccaacaa ttgacccggc aggaaaactg tgttggcata agtgtttgaa gataaatctg
     7981 tttcaaattc gacatgcttt tccaatgatt tcaaactgct gtcaatgggg acacgcgcag
     8041 cgcttagtac atccgaacca tagattttct ggcacactgc caatatctca tcagcctcat
     8101 cagcatggga agatgaatcc atcagaacat aatatagttc ggatttatgg gcatccaact
     8161 gtcgcacatc tagccgagga tcattcagct gtttgacttc atcttgcagg gtttgataga
     8221 attcggcaaa cagattgtcc atcagccact gctccatttt ttcctcaacc tcatcgctgc
     8281 tgtcgggctg gttgatccaa aacagcaatt catccgcact gagataatca gatacaggca
     8341 ttaacgtgtg aatattgtag gcgtgatagg tttcacgata ttgccagtaa gtataaaacc
     8401 agcgagattt ttgttcgagc tcgatatcca ctttgattaa aaaggctgga ggtgagacgg
     8461 acttgtagca ctgctgcagt tcggcgatac cggagaactg ccgggaccag gtcgtcacgg
     8521 tttgggtgct gtcctgtgct tgcagagtgc attgcctgaa ccaggttgaa tctatgggga
     8581 ggggaaaagc gctctcatgg agtttgtccg catcaccctc gatagcgatt ttgcgctcga
     8641 tgcgtccatc tccatatacg gtggtggtgg ttttgatttc acggcatccg gctatccaca
     8701 aaatcagcag cagggccgac aaagtggttt tgctattctt tttcattaag ttcctcgccg
     8761 tttaatagaa agatgatctt gtaatccagc gtttcgttga ccgcgtgcac catatcaggg
     8821 ccgtcctgcc aaagaatctg cagtatttca tgagcagctt gggtgggatc ggtttttttc
     8881 ttttcccgtc cttctctctc cctcgccagc gctgcagaac aaatatccgg catggttgct
     8941 gtcccgatca agcccatctg taatcatcat gtgtaataat acgtgccgaa ttatgtcaag
     9001 tttcgtcatt tggtcagtac agcatggttg ccgggcttgc cggactccga tctgaacgca
     9061 atcctgtacc accatgcacg gacgttcaaa ggaaattaaa agccggtgcc acatggccaa
     9121 aatcctcaag ctctctatta tggaaaaata ttgcttgtat atatctggca ggagttgtaa
     9181 attatgactt gttatccata atatgaccaa ttagtcattt ataaaaccaa tgaggtctgt
     9241 catgtccagg ctccctttat gccgcaagta gcgagaaaga ttggcgcgac agcaggatat
     9301 tctcaccgct gcccgtgagc ttttcctccg ccagggctat cacgagacca ccctggagga
     9361 gatcgcacgc cacgccgagt tcggcaaggg taccatctac aactattttg actccaagga
     9421 ggatctcttc ctcgctatct ttgaccagct gatcggcgag ctcgacacca tcgctcgcac
     9481 caccctgagc gagggcgagg gtgagggccg tcagcgcctg gcggtctatg ccggcaccat
     9541 ggttcactat tccagagaca atgccaagtt gatcgccctg gtcatgcacc agatccatca
     9601 gatcagtcag gagaaaagaa agcctttcct ggcccgttac gctgccgtca tgcaaaaagt
     9661 ctggcagatt atcggcctga cgatccggtc ggagcaggag cagcacggcg cctgtcgtta
     9721 tgatcctatg gcgctggcta ttctcttcga tgggatggtg cgcacctaca acatgacccg
     9781 gttcggcccc ttgcattcga tgcccgagga ggagattgaa caggcagccg acatgatcac
     9841 caccatcttt tttaacggaa tcggaaaacc caaccaagga taaatcccgc aatgttcagg
     9901 attcgtgtca cactcatttt cttgcttgcc attctcatga gccgcagtca ggcattccag
     9961 gctgcaccgc ttccagccgc cagggacacc ctggtgctga gcattgagca ggcaatcgcc
    10021 atggccttgc accagaaccg cgatctgatg atcgccggcg aggaacgcgt caaggcgaac
    10081 gcccaggtcg gcgaagccaa gtccggggcc tttccccagc tcaccgcatc cgggcagtac
    10141 atgcgctaca tcaaaaagca ggtgatgttt ctgccgcccg gcaatgccat caatccgacc
    10201 gacaagaccc tgctcttcga aatcggctcc aacaatgcct actccggcgc ggtgcagctc
    10261 tctcaggcgc tctttagccg caaggtgggg gttgccctgc aaatcgccaa aacctaccgt
    10321 cagctggccg ctgaatcata cagcgccgcc cagcaagcgg tcgtccgtga cacccgcaag
    10381 gcttactatc agatcctgct cgcccaaaag ctggtcgagg ccaaccgtca gggtctggag
    10441 gtcatccaag ccaactatac caatatccag gcgcagtatc gtgtcggcgc cgccgccgag
    10501 tacgatctga tccgcgccga ggtgcagctg gccaataccg agccgctggt gatctctgca
    10561 gaaaacggct atcagctggc ggtcaatgcc ctcaaaaatc tgctggcgat ccccctcgat
    10621 cagcctgtgg tcattgctgg ggagctgagc tatgaagagc tgcccgcgga ggtcgccaag
    10681 caggccgaag cgcaagccct tgcggtcaat ccggccatca agcagttgag cctgcaggag
    10741 cagatgctgg accgcaacat ctcgatcgag aaggcaggca actttccgac cctttacgcc
    10801 gtcggcagct atatctggca gtcgcaggac aacaccttca agttcggcaa ttacaactgg
    10861 gccaatacgg tcaatgccgg ggtttcgctc tcctatcccc tcttcgacgg ctttcgtacc
    10921 aggaaccggg tgcagcaggc caaggtggag cgtaacaagg tgcgttacaa ccgcctcaag
    10981 atggaggagg ggctgagggt gcagatccag gccggcgcgc tgcgcatggc cgaagcgcgc
    11041 cagcggatga gcgggcagga aaaaaccatc gagcaggcgc agaaagcggt gcgcattgcc
    11101 cagacccgct tcaagagcgg cgtcggcacc cagctggagc tgcttgacgc ccaggtggcg
    11161 atgacccgca gtcagaccaa ctatgcccag gcgctttatg actatttggt cagcaagacc
    11221 gagtgggagt ttgccaccgg aaccagtcgt tgacctcttc attcaccaga catcaagtgg
    11281 agttaataca atgaaaagaa gatcattatt agtgtttgga ttgatcctga tttcggcctt
    11341 ttcagccctg gcgctgatgg gtggttgcgg cagagcggaa aaggccgctg tggcgaatcc
    11401 cgtgaaccgc ggcgtcgagg tcgtaccggt ggagaccgcc cgggtcgagc gccagtcgct
    11461 ggcggtaacc aagagctaca ccggtacact cgagggcgaa gcccaggcga atatcgtcgc
    11521 caagatttcg gagcgcatca cggccatcaa tggcagtgtc gggcaatcgg tccagaaggg
    11581 ccggaccgtg atcgcgctcg acaaaagcgg caccacctcg caatactatc aggccgaagc
    11641 gaactacaag aacgcagaga agaacctgga gcggatgaag gcgctgctgg ccgagggcgc
    11701 gatttcgcag cagctgctgg acggcacgca gaccgctttt gatatcgccc gcgccaattt
    11761 cgaggcggcg cgcagtgcgg tcgagctggt ctccccgatc tcgggggtgg tcaccgccct
    11821 caatgtcaat ctgggtgatc tggcccagcc gggtgcagtg ctggccaccg tggccgacat
    11881 ccaccggatg aaggtcatct tcagcatcaa cgaggccgat gtcatgcagc tcgccgcggg
    11941 ccagagcgtc gagatctggt ccgagggcgg gccggaggga cgggcgcagg gcaagatcgt
    12001 gcagatctcg cgttcggccg atgtgcgttc gcgctccttc gagatcaagg ccctcttccc
    12061 caacacggcg gatcagtggt accgccccgg gatgttctgc aaggtggcgg tgacgatctc
    12121 cccacgcaat aatgccctgg tcgtcccggc cgctgcgatt ctcagcgatg gattgacgag
    12181 cagggtctac aaggtggtcg gcggccgggc cattccgcag cctgtccgca ccggcatttc
    12241 ggacggcgcc cgggttgaga ttctcgaggg tgtgaccgag caggagaccg tcgtgaccac
    12301 cggcgccacc aatttgcgcg atagcagcct ggtggctgtc acccccgctc tgcaatgacg
    12361 ctaccactgc tgataagaag gagtagacct ttcgatgaaa ttagctgatg tctccattga
    12421 gcgcccggtc tttgcgacta tgatgatcct ggcgctgctc gtgctgggcc tcttttccta
    12481 tatgaaactc aatattgatc aattcccgga tatcgacttt ccctatgtga ccgccacgac
    12541 ggtactgccg ggagcgggtc cggaacagat cgaaaccgtt gtcaccaaaa aaatcgagga
    12601 cgccgtcaac accatcggcg ggctcgatca tatccagtcg atatcgcagg agaatgtctc
    12661 gatcgtcatc atccagttca agctggaggt ggacggcaag caggcggccc aggaggtgcg
    12721 tgaaaagatc gcggcgatcc gcgccaatct ccccgacgat atcgaggatc cggtgatcca
    12781 gcgcatcgat ccggcgagta caccgatcta caccctggcc ctctcgggga gcctgggcga
    12841 caaggagctc accacctttg ccaaggatgt ggtcaggaaa cggctcgaaa acgtgcccgg
    12901 ggtcggccgc gtcgatctgg tcggcggcgc cgagcgcgag atcgaaattc aggtagatgc
    12961 cgcccggctg caagcctaca acctttcgat ccaggaggtg gtgcaggcga ttgcggcagc
    13021 caatgtcgag gtgccggcgg gcaatctgat tcagggggag cgccagatcc ttttgcgcac
    13081 cacgggaaaa tacaccgatg tcgccgattt tgacaaggtc atcgtcgcct cccccggcgg
    13141 caagcccgtc tacctcagcg agatcgccac ggtggtggac gggatcaagg agcgcacgag
    13201 tctctcgcgc gttgacggca atgccgcggt ggtcctgagc attctcaagc aatcggggag
    13261 caacaccgtg cgggtggcgg acggcgtgac cgcccagatc ggtgcgatcc gcaaggaact
    13321 gccgccgcac attcagctgg cgacggtgcg cgacaacagc accttcatcc gcgattcggt
    13381 acgggatgtc ctatttgaca tcatctacgg cggcttgctg gccgtgatcg tggtcttcct
    13441 ctttttggcc aaccaccggc cgacgctgat cagcgccatc gctctgccga cctcgatcat
    13501 cgccagcttt atcatcatgt ttgcgctcaa attcactctg aacatcctca ccctgctggc
    13561 cctctcgctg gcagtggggt tgctgatcga cgacgccatc gtggtcatcg agaatattta
    13621 ccgacatctg atgaaggggg agagcccgat ggaggcggcg cgcaaggcga ccggcgagat
    13681 cggcctggcg gtcatggcca ccaccttcac catcgtcgcg gtctttgtgc cggtggcctt
    13741 tatgcccggt atcgccggcc gcttctttta ccagttcggc atcaccgtct cggcggccgt
    13801 gctggtctcg ctcttcgtcg ccttcaccct gacgccgatg ctctcctcgc gctggctgcg
    13861 ggaggaagat gaggagctga ccaataccgg ctcgccactg cgccgcggcc tctactggtt
    13921 caaccatttc tttgaggtcc tggccgagaa atacaaaaag gcaatcgact ggtccctgca
    13981 tcaccgcaag accatggtct tcggtgcgat ggcgaccttt ttcttcagtc ttttcctcat
    14041 gcgctttctc gccacggcct tcatgcccag cttcgacatg agccagatga cggtgaccgt
    14101 cacagcagcg cccggcagtt cgctcgaaca gaccagccgg atttgcaaca agatcgagga
    14161 tgccctgcac cagcggcccg aggtgacgat ggtgctgacg accatcggcg ccggcaccga
    14221 tccggtgacc aagggcagtg tcttcgccaa cctggtcaag aaggatcagc gcgacaaggg
    14281 agtggagcag atcatcggcg aactgcgcag cgagctgcag accatccccg gcgccaacat
    14341 cggcttcacg gtacagcagg gcatgggtgg catgcaaaag ccgatgatca tgagcgtgcg
    14401 cggcgaagat ctgaatgtgc tcaagagatt ggccgaccag gtcgagagga tcgtgcgcac
    14461 gaccccgggc gccgtggatg tggagaacag cctcgagacc tccaagccgg aggtgcgcat
    14521 ccgcatcgac cgtgaaaagg cctccgacct tggcgccaat gtcgccctga tcgcctccac
    14581 cgcgcgcgcc atggtcgatg gctacgttgc cagcactttt caggagggcg acgaacaatt
    14641 cgatgtgcgt gtccgtctga acaaggagga c
//
LOCUS       DAOPWU010000044        14615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4210_length_14615_cov_5.041621, whole genome shotgun sequence.
ACCESSION   DAOPWU010000044 DAOPWU010000000
VERSION     DAOPWU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4210_length_14615_cov_5.041621"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..720
                     /locus_tag="PKM23_04115"
     CDS             307..720
                     /locus_tag="PKM23_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNY90688.1"
                     /translation="MKAKMVIGVVVIVGALIYLMVSGFRDSSVYYMTVSELSGQKAEV
                     INEGLRVSGYVDPQSIAWNAEKIELDFTLFEGSDSLRVHYKGVIPDQLADAQGVLAEG
                     KLTADGRLEATRLMLKCPSKYEAKLDEKPGAGSTQ"
     gene            complement(799..1404)
                     /locus_tag="PKM23_04120"
     CDS             complement(799..1404)
                     /locus_tag="PKM23_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNY90689.1"
                     /translation="MKKGLKTALIVIGILLVLGLLIASPYNNLVRLDEGVKNAWAQVE
                     NQLQRRMDLIPNLVKTVEGYANFERATLEAVINARAKATSTQINAGDMNPAQLQQYME
                     AQNGLTSALGRLMVVVEKYPELKANENFLRLQDELAGTENRIAVERKRYNDQVQTYNQ
                     SVRKFPVVLIAGMLGFGQKPYFEAPAEANAAPTVDFSGLNK"
     gene            complement(1420..2148)
                     /locus_tag="PKM23_04125"
     CDS             complement(1420..2148)
                     /locus_tag="PKM23_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90690.1"
                     /translation="MAKKLDSPQSIANEFTEDVKAIYGADLVAVMLYGSALSGHWDPK
                     RSNINFLIVLSEVAITRLDAAFPLVEKWRKRLRTLPIFMTKNYIAGSLDSFPVEFLHM
                     KRRYQLLYGEDVLAPLVIPHANLRLQCEEQIKGKLLHLREEYLATLGKRYRLQALVTL
                     TLPPFAVLFKSLLALKDAEIPALHRDIIMRTAELYGLDRDLFQQLIQAFERKGKFSLV
                     EMNRLAAAYITEINKLTLLIDQME"
     gene            complement(2225..3031)
                     /locus_tag="PKM23_04130"
     CDS             complement(2225..3031)
                     /locus_tag="PKM23_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNY90691.1"
                     /translation="MRKFLSLALFFLFILPLRAEESFPQRRGAVNDFAEVIPAAEEAE
                     IEALAIEVWDKAGVALVICTMPTIGDADYQSYANELYKAWGIGKQGEDKGLLIFNVTD
                     IRKIWIETGYGVEGFINDARAGDVYRQHLVPNFQQGNFGRGFLEAMQAFAGMVSEEYG
                     ITFDGTTIAPRRGGNPDDGMEDLIVFLVIGFIFLLIILSNHNRSGWGSGGDWGSGPGR
                     RSGSRGWDGSGWGSSSWGGGGWGGGFSGGGGGFGGFGGGGSGGGGAGGGY"
     gene            3390..6296
                     /locus_tag="PKM23_04135"
     CDS             3390..6296
                     /locus_tag="PKM23_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90692.1"
                     /translation="MRNPAFELAAVVLCALFVLGTPGAPVQAQPTSLQPLAQLLRADG
                     TLDLGLGRNVALDPSGFEMTIDDQGRPRFTPARAVKPSAPEAVEKWSTEYTFSGADNI
                     VTALAIHNQELYIGGYFSAIGPVFANRIAKWDGFTWTPLGPGVDGNVHAILVTDTGVY
                     IGGDFIYAGDLFCQRIAKWDGARFSALGEGFNGKVRALAMSNGTLYAGGEFTSSGSTT
                     VNRIARWTGSSWSPVGAGCNGPVLSFAVNGSDLYVGGDFTFIGSTPVNRVGRWTGTAW
                     FTLASGTNNIVRALTFSNGSLYAGGDFTTAGTVTVNYVARWNGAAWSAMGSGADQRVT
                     ALAADEGTIYAGGHFYNIGGVSALRLASWDGSTWSALGSGVNSFVYALAFDDGQLFVG
                     GDFSAAGAVTAHHIVGWNGHNYGSLHFGKGFNGPVHAILMDGTTAYVGGSFTQGGGIA
                     ASNIIKYDGSTWSALGNGISGPVYALAKQGTDIFAGGRFNSASSIAASNIARWNGSAW
                     FSLGSGINNGSPGTGTVYALASNGIDSLVAGGEFSNAGGLNTPRVALWNGGAWKTIGL
                     GMDNTVYTLAYESFMFYAGGKFKNTQGGGYFLNYAALWSGDRQAWYAMGTGVDSTVYV
                     MKPGAAGVLYVGGNFTKADGLPARRLARWEGSLWDGAVSTGANGRIRAIDVLGTRLYI
                     GGDFSEAGGLPANRFAILDLSSDSWSAPGNGLDSTVYALGNGDLYLGGAFTAAGSRPS
                     FHFAQWLESSFVYMQVRLFLQGAYNPAAHAMTTTLNSAGHIPLVSPYPDHRSVSYIPA
                     NVTDWVMVELRTGGGTVAGQKSFFLNRNGYLVDDDGENTQVIFEGVTDGDYSIIIHHR
                     NHLKVMSANTVALTSGGATAFDFTTSLSQSYEFDSAGRTCVELEPGVWGFWAGDINQD
                     GAITTRDHVVWYDANYAGESGYCLSDIDLNGSPAASDFDLWQTNARIDPRCQVP"
     gene            complement(6395..7072)
                     /locus_tag="PKM23_04140"
     CDS             complement(6395..7072)
                     /locus_tag="PKM23_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HNY90693.1"
                     /translation="MRSNSSKSGTFHGLYAELFALYGPRGWWPLIGLAEESRPGCCIR
                     GYHPLDYELPATPAQVFEICAGAILTQSVNWTSVEKALRSLHALNALQPARLLALGDD
                     VLTAAIRPAGYFNQKAKKLRLFSAFFAGLDGRIPDRQELLTLWGIGPETADSMLLYAF
                     KVPTFVVDAYTRRVLIRRGLIRGSESYDDIKRLFEENLPRDLALFQEYHALIVEHAKQ
                     LRGKPGE"
     gene            complement(7053..7988)
                     /locus_tag="PKM23_04145"
     CDS             complement(7053..7988)
                     /locus_tag="PKM23_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY90694.1"
                     /translation="MKRSALLLHFRTLALFVLMLLLSGAVTLLFGAEGEGSRPMTFFS
                     VWALPRVWVGAILGFLGLVLLMTRKATAWVRFAALVIIFLAFSVVPFLPWGKFASGMG
                     LHPSPMCSVEKPLMFIQMGRSVPIIFISILGFILLMTVIANKLFCGWNCPIGALQELL
                     HRIPLPRGWKGKLPFRITNAIRIALFLLFVALLFGMGFSLYAWINPFEFLHWQMEWAI
                     IPAFVITFIGALFFYRPFCYLVCPIGLLTWLAEHISILRVRLDKQACTDCMLCVKKSP
                     CPTVPSILEGKKSRPDCHACGACIEACPENALKFK"
     gene            complement(8015..8899)
                     /locus_tag="PKM23_04150"
     CDS             complement(8015..8899)
                     /locus_tag="PKM23_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNY90695.1"
                     /translation="MRNHMQPLLLLLLLAGLFISCKDKKAIESGIEFVKVNGGQFQMG
                     DTFGDGDSDEQPVTETRVGDFYLGKTEVTVAQFRRFVDATGYVTEAEKEGSGWAWTGE
                     KIDRIPGADWRNSGFEQGDFHPVINVSWNDAMAFCDWAGCRLPSEKEWEYACRSRGQK
                     IKYSWGNDFPRGAKGGNIADESGAAGYPLRSWFKGYSDGFVFTAPVGRFEANGLGLVD
                     MTGNVLEWCSDLYEEYLGYLPPNREFSSLPMERSRVLRGGSWADDPAYSRCSERAGQD
                     PSFAYPFIGFRVASSGKP"
     gene            9073..9885
                     /locus_tag="PKM23_04155"
     CDS             9073..9885
                     /locus_tag="PKM23_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90696.1"
                     /translation="MKRSILLWFLALVITLASAVYQRMTGPTHPERGNFVLEGQSAAY
                     KLPCAHVIGEDHSVSLPAGSGAARAALFYRRLNSNDDWSTVPMPAAGDRFRADLPEQP
                     TGGKMQYYIEVEEQGQIVRIPETGVVVTRFRHSVPASVLIPHIFIMFFGMLLSTRAGL
                     EALRKGADLKAWTWWTLAVIFIGGMIFGPLVQKYAFGEYWTGIPFGTDLTDNKTLIAL
                     VAWIAAALAVRRNPQARKWVFAAALVTLIAFAIPHSLMGTEVDHLKAAESAR"
     gene            10115..11077
                     /locus_tag="PKM23_04160"
     CDS             10115..11077
                     /locus_tag="PKM23_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90697.1"
                     /translation="MFADLAALDLDQLLAEPDTGTAPAACIISVGSGGRGCGKTALCW
                     LIALTLSRKGHRTILLDSDLAAAGLYGRTRLPVSDQFLHHFLQRRDADINILPRPVDN
                     KNLYLITGSPSVQSHTRLSLAAKQKILLHLHRLSAEYIVIDCGQGPSYPHLDLFLAAD
                     LPIVVARLPNSSLLESYQFIRTGFFRRIQHRSRHWPDLFGRLSRCGDITRAGEIRTLP
                     AFLARHGEAHPHLCTVIQEGLDGYKPRLVINRAGAGMERRRIQAMQELLLQAMGITLS
                     SWGEIGEDENIKKSEAAGQVGMLLRGSAAMEVAELVRQRLLTRA"
     gene            complement(11166..12374)
                     /locus_tag="PKM23_04165"
     CDS             complement(11166..12374)
                     /locus_tag="PKM23_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90698.1"
                     /translation="MFCKIAAMKSPAALILLALALAGCSEQPGQPGRDTAIRLNLRFL
                     PPGERSAFPGTEEETASLGKSARLVEDQVLLLHCDDESLAVATDASLYRNNFQLFSIE
                     YANSENAALKKALVFNGDNSYGLSTAYESKAGILNGTHGFSFSCRLQMTGAGMIGEKR
                     IFDRHDPLGGYTIGVITSPLAYTPRLFLKIKQEGVETILTSKSVLTPGTWYKISAGYD
                     QNRLAISINDKADTTLAHRGLVSPSARPTVLGAGWNGDLIGYPFNGLLDEISLTTTVD
                     YYDFEQIRVAVVDLSDYDNISDFYRTKGMEMADSLYHMEESGTGAPSWEAYGQVFRDI
                     GLEVVYNQNLGIDKGFAAGTIRGVEGGNLIFVGAIQDGAVIYSGGGFVWTTGDAMADV
                     VIELESYEGK"
     gene            complement(12395..13519)
                     /locus_tag="PKM23_04170"
     CDS             complement(12395..13519)
                     /locus_tag="PKM23_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90699.1"
                     /translation="MIEQRKAPLRLLTALILAACPALFAQPAMLQRAREGTASASAVL
                     NLWQVEEEQVRQIAIPLTFLYPVNEQLQLTIGAGPAFSTFEGSGEAALNGFSDTRFGG
                     SYLFKNERLLATFGINLPSGKHALNGEELSVANILALHAFDFTVPILGQGLDVSAGLV
                     SAFPLGGMVAGLGAGFLMRGAFEPFENGSVKYNPGEEITLSAAVDLPLQRRKKLMFDA
                     NYTIYTADKAGGSKLFQAGNRITLQAMAWFPGESAGVLLMARDRIRAANKIVSNDDLI
                     PERQNSNGNELELLAMVSLPRSAYTTLRGVIEGKIYSNNAYDIGGATIGGIGAGLSKI
                     FTPHLSLELDGRFYFGSMETGIDKVGLTGLRATGGLKYRL"
     gene            complement(13553..>14615)
                     /locus_tag="PKM23_04175"
     CDS             complement(13553..>14615)
                     /locus_tag="PKM23_04175"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNY90700.1"
                     /translation="QALQHYSRYTQLNPARKMRQTVEGRMQVLVRQQMAAQIKALLEQ
                     EEALEASPAPENSIAVLYFTDLSGNPDFVPLRKGLAEMLITDLAQVKSLQVVERARMQ
                     MLLDEMGLGMTGLVKEETAPRMGRLLKAGRIVQGTFSGADAKMLKIDALLATVGVETP
                     AAAEKISGSLEEFYQLQKDLVFNLIDVMGLRLSRGEREAIQRIPTKNLTAFMAWCRGL
                     DEEDRGNWQGAQEQFRAAVSADPAFQSAIDAIGRSAAFASFSPRPETAPPSLLAEAGG
                     RDDRRESTRLPSGGAAAVPSASAGDLITIDLLSRAAAQITPGFVPGVESRKPTTESST
                     IGIGTGIPLEVRIQLPVKP"
BASE COUNT         2808 a         4519 c         4518 g         2770 t
ORIGIN      
        1 caacaggatc aacaggattt tgcgcatgga agcctccttg gtttgaacca aatttagcca
       61 aatcgagggc aaatacaagc ggaattatat ccctgcagcc cttcggggcg gcaagggtgt
      121 cgggcgcccc ggagtcgccg gtaaaagtct gcagacggcc gggtgcggcc aggcggcccg
      181 atcgccggat tgaggctgca ggcttctgca cccgatcccg gctgcagctc atatcccgtc
      241 ttgactctca cccgcgaatt atgtacattg agccgttgaa agaaatatta cgcatctgga
      301 gtggaaatga aagcgaaaat ggtcatcggg gtggtcgtca tcgtgggcgc cctgatctat
      361 ctaatggtga gcggctttcg cgacagctcg gtctactaca tgacggtttc ggagctgtcc
      421 ggccaaaagg cggaggtgat caacgagggg ctgcgggtga gcggctacgt cgatccccag
      481 tcgatcgcct ggaacgctga aaagatcgaa ctcgatttca ccctcttcga ggggagcgac
      541 agtctgcggg tccattacaa gggagtgatc cccgaccagt tggctgatgc ccagggggtt
      601 ctggcggagg gcaagctgac ggccgacggc cggctggagg cgactcgcct tatgctcaaa
      661 tgcccttcca aatacgaagc caaactcgac gaaaaaccgg gcgcgggcag cactcagtag
      721 ggccaagagc gcagagacgc aaaaagccgg cgaaggcata gcctttgccg gcttttttaa
      781 tgtcaggccg cggtgcggct atttgttcag gccgctgaaa tcgacagtgg gcgccgcatt
      841 ggcctcagcc ggggcctcga aatagggctt ctggccgaag ccgagcatgc cggcgatgag
      901 gacgaccggg aacttgcgca cagactggtt gtaggtctga acctggtcgt tgtagcgctt
      961 gcgttcgacg gcgatgcggt tctcggtgcc cgccaattca tcctgcaggc ggaggaaatt
     1021 ctcgttggcc ttgagttcgg gatacttttc gacgacgacc atcagacgtc cgagggcgct
     1081 ggtgaggccg ttctgggcct ccatgtactg ctggagctgg gctggattca tgtcgccggc
     1141 gttgatctgg gtgctggtcg ccttggcgcg ggcgttgatg acggcttcga gggtggcgcg
     1201 ttcaaagttg gcatagccct cgaccgtctt gaccagattg gggatcaggt ccattctgcg
     1261 ctgaagctga ttctccacct gagcccaggc gttcttgacc ccttcatcga ggcggaccag
     1321 attgttgtag gggctggcga tgagcaggcc cagaacaagc aggatcccga tcacgatcag
     1381 ggctgttttc aatccttttt tcattttttc ctccgttggc tattccattt gatctataag
     1441 caaggttagt ttattgattt cagtgatata ggccgcagcg agcctgttca tctccaccag
     1501 cgagaacttg cccttgcgct cgaaggcctg gatgagctgc tgaaagagat cgcggtccaa
     1561 accgtaaagt tcggcagtgc gcatgatgat gtcgcgatga agggcgggga tctccgcatc
     1621 cttgagggcg agcagcgact tgaagagcac ggcgaacggt ggcagggtga gggtgacaag
     1681 cgcctggagc cggtagcgct tgcccagggt ggcgagatac tcttcgcgca ggtgcaggag
     1741 cttgcccttg atctgttcct cgcactgcag gcgcaggttg gcatgaggga tgacgagggg
     1801 tgcgagcaca tcctcgccgt acaaaagctg gtaacggcgt ttcatatgga gaaactcgac
     1861 cggaaagctg tcgagcgagc cggcgatata attcttcgtc atgaagatgg gcagggtgcg
     1921 caggcgcttg cgccactttt ccaccagggg aaaggcggcg tcgaggcggg tgatcgcgac
     1981 ctcggagagg acgatgagaa aattgatatt tgatcttttg ggatcccagt gtccgctgag
     2041 ggcgctgccg taaagcatga cagcgacgag atccgcgccg taaatggcct tcacatcctc
     2101 ggtgaattca ttggcaatgc tttgtggcga atccagcttt ttcgccatgc ttttctcctt
     2161 atatccatcc agtcctgtga gcgggcagcc tgacctccgc gtttcgagcc ggttaaggcg
     2221 ggcactagta tcccccgccg gcgccgccgc caccgctgcc gccgccacca aatccgccaa
     2281 agccgccgcc acccccgctg aaaccgcctc cccagccgcc gccgccccag ctgctgctcc
     2341 cccatccgct tccatcccag ccccggctgc ccgaccgcct tccgggtccc gagccccagt
     2401 cgccgccgga gccccagccg gaacggttgt gattgctcag gataatgagc agaaagataa
     2461 agccgatgac caggaaaacg atcagatcct ccatgccatc gtccgggttt cccccccggc
     2521 gcggggcgat ggtcgtcccg tcgaaggtga tgccatactc ctcgctgacc atgcccgcaa
     2581 aagcctgcat cgcctcgaga aaaccccggc cgaaattccc ctgctgaaag tttgggacca
     2641 gatgctggcg gtagacatcc ccggcgcggg catcgttgat aaagccctcg acaccgtaac
     2701 cggtctcgat ccagattttg cggatgtcgg tgacgttgaa gatgaggagc cccttgtcct
     2761 ccccctgttt gccgatgccc caggccttgt agagctcgtt ggcataggac tggtaatcgg
     2821 catcgccgat ggtgggcatg gtgcagatca ccagggcgac gccggccttg tcccagacct
     2881 cgatggccag ggcctcgatc tcggcctctt cggcggcggg aatgacctcg gcgaaatcat
     2941 tcacggcccc ccggcgctgg ggaaagctct cctccgcacg cagcggcaaa atgaaaagaa
     3001 aaaaaagggc cagggaaaga aatttacgca tgttcgcgga ttccggatgc agtcggtttt
     3061 ttaataaagt acatcatttc gcaggtaaat gcaagcaaat tgcgcagggg ccgcctcctc
     3121 caccgcggcg gctgctgccg gcgtcctggc cggtaaaacg cgtgcggaaa gggccggcgg
     3181 cggcctgaga aagaagaggc cggacgggaa aagcacttgc aggggccggt ttttttgcgt
     3241 attattgctc tgcacggcgt ttttttgcgc aatcctgagt gattgcatgt acataacagc
     3301 atacaagata gtgtccggtt cacctctcca gcacctgaat cgacatccca aagaggccgt
     3361 taaccatcag tataggaggc agtcaggaca tgagaaatcc ggcctttgag cttgcagccg
     3421 ttgtgctttg cgcactattt gttttgggaa ctccgggggc tcccgtacag gctcagccca
     3481 cttctctcca gccccttgca cagcttttac gcgctgatgg cactctggac ctcggcctcg
     3541 gccgcaacgt cgccctcgat ccctccggct ttgaaatgac catcgacgat cagggccggc
     3601 cgcgcttcac ccccgcccgc gcagtcaaac cctctgcccc cgaggcagtt gaaaaatgga
     3661 gcaccgaata caccttttcc ggcgccgaca atatcgtcac cgccctggcc atccataacc
     3721 aggagcttta catcggcggt tatttcagcg ccatcggccc cgtcttcgcc aatcgcattg
     3781 ccaaatggga cggcttcacc tggacacccc tcggtccggg cgtggacggc aatgtgcacg
     3841 ccatcctggt gaccgacacc ggcgtctata tcggtggcga tttcatctat gctggcgacc
     3901 tcttctgcca gcgcatcgcc aaatgggacg gcgcccgttt cagcgccctc ggtgagggct
     3961 ttaatggcaa agtgcgcgcc ctggcgatgt ccaacggcac cctctatgcc ggcggcgagt
     4021 tcacctcgag cggctcgacc accgtcaacc gtatcgcgcg ctggaccggc agcagctgga
     4081 gcccggtcgg cgccggctgc aacggcccgg tcctctcctt cgccgtcaac ggcagcgacc
     4141 tctacgtcgg cggtgacttt accttcatcg gttccacgcc cgtcaaccgc gtcgggcgct
     4201 ggaccggcac tgcctggttc acccttgcct cggggaccaa caacatcgtc cgcgccctca
     4261 ccttcagcaa cggctcgctc tatgccggcg gagatttcac cactgccggc accgtcacgg
     4321 tcaactatgt cgcccgctgg aacggcgcgg cctggagcgc catgggcagc ggcgccgatc
     4381 agcgcgtcac cgccctggcg gccgacgagg gcaccatcta cgccggcggc catttttaca
     4441 atatcggcgg cgtcagcgcc ctccgcctcg ccagctggga cggctccacc tggagcgccc
     4501 ttggcagcgg cgtcaacagc tttgtttacg ccctcgcctt cgacgatggc caactttttg
     4561 tcggcggaga tttttcggcc gccggggccg tcaccgctca tcatatcgtc ggctggaacg
     4621 gccacaacta cggctccctg cacttcggca agggcttcaa cggccccgtt cacgccatcc
     4681 tcatggacgg caccacagcc tacgtcggcg gctccttcac ccagggcggg ggcatcgccg
     4741 ccagcaatat catcaagtat gacggttcca cctggagcgc cctcggcaac ggcatcagcg
     4801 gcccggtcta tgccctcgcc aaacagggta cggacatctt cgccggcggg cgctttaaca
     4861 gcgccagcag catcgccgcc agcaatatcg cgcgctggaa cggctccgcc tggttcagcc
     4921 tcggcagcgg catcaacaac ggctcgccag ggaccggcac tgtctacgcc ctggccagca
     4981 acggcatcga ctcactggta gccggtggcg aattcagcaa cgccgggggg ctcaacaccc
     5041 cgcgcgtcgc gctctggaac ggcggggcct ggaagaccat cggcctgggc atggacaaca
     5101 ccgtctacac cctcgcctac gagagcttca tgttctacgc cggcggcaag ttcaagaaca
     5161 cccagggcgg cggctatttc ctcaattacg ctgccctgtg gtccggcgac cgtcaggcct
     5221 ggtacgccat gggcaccggg gttgacagca ccgtctacgt catgaagccc ggtgccgccg
     5281 gggtccttta cgtcggcggc aatttcacca aggccgacgg ccttccggcc agacgcctcg
     5341 cccgctggga gggctccctc tgggacggcg ctgtcagtac gggcgccaac ggccgcatcc
     5401 gcgccatcga cgtcctcggc accaggctct acatcggcgg cgatttcagc gaagcgggcg
     5461 gcctcccggc caaccgtttc gccatcctcg acctgagttc cgactcctgg agcgcacccg
     5521 gcaacggtct cgacagcacg gtctatgccc tcggcaatgg cgacctttac ctcggcggag
     5581 ccttcactgc ggcaggttcg aggccatctt tccacttcgc ccagtggctg gagagcagct
     5641 ttgtctatat gcaggtcagg cttttcctgc agggggccta taaccctgca gcccacgcca
     5701 tgaccaccac cctcaacagc gccggccaca ttcccctggt ttcgccttat ccggatcatc
     5761 gcagcgtcag ttatatcccc gccaacgtca ccgactgggt gatggtcgaa ctgcgcacgg
     5821 gcggcggtac agtcgccggg caaaaaagtt tcttcctgaa caggaacggc tatctggtcg
     5881 atgacgacgg cgagaatacc caggtgatct ttgaaggggt gaccgatgga gactattcga
     5941 tcatcataca ccaccgcaac cacctcaagg tgatgagcgc caacaccgtc gccctcacca
     6001 gcggcggggc gaccgccttc gatttcacca ccagcctcag ccagagttat gaattcgaca
     6061 gcgccggtcg cacctgcgtt gagctggagc ccggggtctg gggtttctgg gcgggggata
     6121 tcaaccagga cggcgccatc accacgcgtg accatgtcgt ctggtacgat gccaattatg
     6181 ccggcgagtc gggctactgc ctcagcgaca tcgatctcaa cggctccccg gcggcttccg
     6241 attttgacct ttggcagacc aacgcccgga tcgacccccg ctgccaggtc ccctgatcca
     6301 ttttttcctt ctcccccgac acaaaaaaag ggtgcgccga tgcgcaccct ttttttatgc
     6361 tttgaggctg gcgttcccgc acagacggtg tctctcactc ccctggcttg cccctcagct
     6421 gcttggcatg ttcgacgatc agggcgtggt actcctgaaa aagggccagg tcgcgcggca
     6481 ggttctcctc gaagagccgc ttgatatcat cataggattc acttcccctg atcagcccgc
     6541 gccggatcag gacgcggcgg gtgtaggcat cgaccacaaa ggtcggcacc ttgaaggcgt
     6601 agaggagcat cgaatcggcg gtttccgggc cgatgcccca aagggtgagc agttcctggc
     6661 ggtcggggat gcggccatcg aggccggcga agaaggcgct gaagagacgc agttttttgg
     6721 ctttctggtt gaaatagccg gcggggcgaa tggccgcagt caggacatcg tcgcccagcg
     6781 ccagcaggcg ggcgggctgg agggcattca gggcgtggag gctgcgcagg gccttctcga
     6841 ccgaggtcca gttcacactc tgggtcagga tggccccggc gcagatctcg aagacctgcg
     6901 ccggagtggc gggcagctca tagtcgaggg ggtggtagcc ccggatgcag cagccggggc
     6961 gcgactcctc cgccaatccg atcaggggcc accagccccg gggcccgtag agggcaaaca
     7021 gctctgcgta gaggccgtgg aaggtgcccg atttacttga atttgagcgc attttccgga
     7081 caggcctcga tgcaagcccc gcaggcgtgg cagtcggggc gtgatttttt accctcaagg
     7141 atggagggaa ccgtcggaca ggggctcttt ttaacacaga gcatgcagtc ggtgcaggcc
     7201 tgcttgtcca ggcgcacgcg caaaatggag atatgttcgg ccagccaggt cagcagcccg
     7261 atcgggcaga ccaggtaaca gaaggggcgg tagaaaaaga gggcgccgat gaaggtgatg
     7321 acaaaggcgg gaatgatcgc ccattccatc tgccagtgaa gaaactcaaa gggattgatc
     7381 caggcataga ggctgaaccc catgccgaaa agcaaggcga cgaagagaag aaaaagcgca
     7441 atgcggatgg cattggtgat ccggaaagga agcttgccct tccagccgcg cggcagggga
     7501 atgcggtgga ggagctcctg cagggcgccg atcgggcaat tccagccgca gaagagcttg
     7561 ttggcgatga cggtcatgag caggatgaag ccgaggatcg aaataaagat gatcggcacg
     7621 ctgcggccca tctggatgaa catcagtggt ttctcgaccg aacacatcgg gctcggatgg
     7681 agccccatcc cggaagcgaa tttgccccag ggcaggaagg ggacgacact gaaggcgaga
     7741 aagatgatga ccagggcggc gaagcgcacc caggccgtgg ctttgcgggt catgagcagg
     7801 acgaggccga ggaagccgag gatggccccc acccagacgc ggggcagagc ccagaccgaa
     7861 aagaaggtca tcggccggct gccttcgcct tcggcgccga agagcagggt gaccgcgccg
     7921 gagaggagca gcatgagcac gaaaagggcc agtgtgcgaa agtggaggag cagagcggag
     7981 cgtttcatga tattcccttt ctgttctgta tgggtcaggg tttgccggac gaggcaacgc
     8041 gaaaaccgat aaaggggtag gcgaaagagg gatcctggcc ggcccgctcg gaacagcggc
     8101 tgtaggccgg atcatccgcc caggagccgc cgcgcagcac ccgcgaacgc tccatgggca
     8161 gggagctgaa ctcccggttc ggaggcagat agccgaggta ttcctcatag agatcggagc
     8221 accactcgag cacatttccg gtcatgtcca caagccccag gccgttggcc tcgaagcggc
     8281 cgacaggagc agtgaagaca aagccgtcgc tgtagccctt gaaccagctc cgcaggggat
     8341 agccggcggc cccgctctcg tcggcgatgt tgcccccttt ggcgccgcgc ggaaaatcat
     8401 tcccccagct gtatttgatc ttctggccgc ggctgcggca ggcatattcc cactcttttt
     8461 ctgagggcag ccggcagccc gcccagtcac aaaaggccat ggcatcattc cagctgacat
     8521 tgatcaccgg atggaagtcg ccctgctcga agccggaatt gcgccagtcc gcgccgggaa
     8581 tgcggtcgat cttttccccg gtccaggccc aaccgctgcc ctccttctcc gcctcggtca
     8641 cataaccggt cgcatcgaca aaacggcgga actgcgccac ggtcacctcg gtcttgccga
     8701 ggtaaaaatc cccgaccctg gtctcggtga cgggctgctc atcgctgtcg ccgtcgccga
     8761 aggtgtctcc catctggaat tggccgccgt ttaccttgac gaactcgatc ccgctctcga
     8821 tggccttttt atccttgcac gagatgaaga ggccggcgag caggaggagg agaagcaggg
     8881 gctgcatatg gtttcgcata cattccctct tgcgatatcg ttgagatgaa gccgggtggt
     8941 gaatgctcgt ttatccaatg tacaattttc cgctgtcatt atcaaccctt ttgcggtgtg
     9001 tggaataagc cgcctgcgcg gtctgttttt gctaatgcat gccctgaaac aaatccatcc
     9061 aatgaggttt ctatgaaacg ctccattctc ctctggtttc tcgccctggt catcaccctc
     9121 gcctccgcag tctaccagcg catgaccgga cccacccatc ccgagcgcgg aaatttcgtt
     9181 ctggagggcc agagcgccgc ctacaaactg ccctgcgccc acgtcattgg cgaggaccac
     9241 agcgtcagct tacccgccgg cagcggggcc gcccgggctg ccctttttta tcgccgcctc
     9301 aactcgaacg atgactggag cacggtgccg atgcccgcag ccggggaccg cttcagggcc
     9361 gatctgccgg aacagccgac cgggggcaag atgcagtact acattgaagt cgaggagcag
     9421 gggcagatag tgcgcatccc ggagaccggc gtggtcgtca cccgctttcg ccattccgtc
     9481 cccgcctccg tgctgatccc gcacattttc atcatgtttt tcgggatgct cctctccacc
     9541 cgcgccggcc tggaggccct cagaaaaggg gctgacctga aggcgtggac ctggtggacc
     9601 ctggcggtca tctttatcgg ggggatgatc ttcggcccgc tggtgcagaa atacgccttt
     9661 ggcgaatact ggaccggcat ccctttcggc accgatctga ccgacaataa aaccctgatc
     9721 gccctggtgg cctggatcgc cgccgcactg gccgtgcgca ggaatcccca ggcgcgcaag
     9781 tgggtgttcg ccgccgccct cgtcaccctg atcgccttcg ccatccccca cagcctgatg
     9841 ggcaccgagg tggaccatct caaggcggcg gagagcgccc gataaacagt ccggcccggc
     9901 cggaaaaacc gtgttaaagg ggaggggagc tcggccgtca ctaaaagagg acaggtgtca
     9961 tgcacagtgc gcatacttta cccggtcgcg cggggcaggc ggtgaggatg caatttaaca
    10021 tactgttttt catgacctga ttgggccggc aaacagactg ttcctggcgc gggtcttgca
    10081 ttagtgctcg tcaagaccaa acaaaggata ctccatgttt gccgatctcg ctgcactgga
    10141 cctcgaccag ctcctcgccg agcccgatac cggcacagcc ccggccgcct gcatcatctc
    10201 cgtcggctcg ggcggccggg gctgcggcaa aaccgccctc tgctggctca ttgccctgac
    10261 cctctcccgc aagggccaca ggaccatcct gctcgacagc gatctcgccg ccgccgggct
    10321 ctacggtcgc acccgccttc cggtcagcga tcagttcctg caccattttc tccagcggcg
    10381 cgacgccgat atcaacatcc tgccgcgtcc cgtcgacaac aagaacctct acctcatcac
    10441 cggctcgccg tcggtgcaaa gccatacccg cctctccctg gccgccaagc agaagatcct
    10501 tctgcatctg cacaggctga gcgcagagta tatcgtgatc gactgcggcc agggacccag
    10561 ctatccccat ctcgaccttt ttcttgcggc ggacctcccc atcgtcgtcg cccgtctgcc
    10621 caactcctct ctccttgaat cctatcagtt catccgcacc ggctttttcc gccgtatcca
    10681 gcaccgctca cgccattggc cggatctttt cgggcgtctg agccgatgcg gggatatcac
    10741 ccgggcgggg gagatcagaa ccctgcctgc ctttctcgcc cgccatggtg aggcgcaccc
    10801 ccacctctgt acagtcatcc aggaggggct ggatggctac aagccgaggc tggttatcaa
    10861 ccgcgcgggg gcgggcatgg agcggcggcg catccaggcc atgcaggaac tcctcctgca
    10921 ggcgatggga atcactctct cgtcctgggg tgaaatcgga gaggatgaga acatcaaaaa
    10981 aagcgaggcg gcagggcagg tcggcatgct actgcgcggc agtgcggcga tggaggtcgc
    11041 agaactggtc cggcagcggc tgctcacccg cgcctgaaac aatcaaaaat taaaaaggcc
    11101 ggcgtcaccg ccggcccgag ccatactcca cctcaaaagg cggccgccgc cccctcattc
    11161 aggattcatt tcccttcata actttcgagt tcaatcacca catcggccat ggcatcgccg
    11221 gtggtccaga cgaaaccgcc gccgctgtag atcactgcgc cgtcctgaat ggcgccgacg
    11281 aagatcaggt tccctccttc aacaccgcgg atggtcccgg cggcgaagcc cttgtcaatt
    11341 cccagattct ggttgtaaac cacctcgagg ccaatatccc ggaagacctg cccataggcc
    11401 tcccaggagg gcgccccggt gcccgactcc tccatgtggt agagtgaatc ggccatctcc
    11461 atccccttgg tcctgtaaaa gtcgctgatg ttgtcatagt cggagagatc gacgacagcc
    11521 acgcggatct gttcaaagtc gtaatagtcc accgtagtgg tcagcgagat ttcatccagc
    11581 aggccgttga aggggtaacc gatgaggtcg ccgttccagc cggcgccgag gaccgtggga
    11641 cgggccgagg gggagaccag gccgcggtgg gcgagggtgg tgtcggcctt gtcgttgatg
    11701 gagatcgcca gccggttctg gtcatagccg gcgctgattt tataccatgt gcccggggtc
    11761 aggaccgact tgctggtgag gatggtctcg acgccctcct gcttgatttt cagaaagagc
    11821 ctgggggtgt aggcgagggg cgaggtgatg accccgatgg tgtatccgcc cagggggtcg
    11881 tgccggtcga agatgcgctt ctcccctatc atgccggcac cggtcatctg caagcggcag
    11941 gagaaggaaa aaccgtgcgt gccgttgagg atccccgcct tgctttcata ggctgtgctg
    12001 aggccgtacg aattgtcgcc gttgaaaacc agggcttttt taagcgcggc gttttccgag
    12061 ttggcatact cgatgctgaa gagctgaaaa ttgttgcggt agaggctggc atcggtcgcg
    12121 acggcgaggc tttcgtcgtc gcagtggagc agcagcacct ggtcttcgac cagccgcgcc
    12181 gatttgccca ggctcgccgt ctcctcctcc gtcccgggaa aggcggagcg ctcgccgggg
    12241 gggagaaagc ggaggttgag acgaatagcg gtatcccgtc caggctggcc gggctgctcg
    12301 ctgcatccgg ccagagcgag ggcgaggagg atcagggccg cggggctttt cattgctgca
    12361 attttacaga acatagaggg actcctgaac ctgattaaag acggtacttg aggccgcctg
    12421 tcgcgcgcag gccggtgagg ccgaccttat cgatgccggt ctccattgaa ccgaagtaga
    12481 aacggccatc cagctcgagg gagagatggg gggtaaaaat cttgctcagg ccggcgccga
    12541 tgccaccgat ggtggctccg ccgatgtcgt aggcgttgtt gctgtaaatt ttgccctcga
    12601 tcaccccgcg cagggtggta taggcgctgc gaggcagcga gaccatggcg agcagctcca
    12661 gttcattgcc gttgctgttc tgacgctccg ggatgagatc gtcgttgctg acgattttgt
    12721 tggcggcgcg gatgcggtcg cgggccataa gcagcactcc ggcgctctcg ccggggaacc
    12781 aggccatcgc ctgcagggtg atgcggtttc ccgcctggaa gagtttgctc ccccccgcct
    12841 tgtccgcggt gtagatagtg taattggcat cgaacatcag ttttttacgg cgctgaaggg
    12901 gaaggtcaac cgccgccgag agggtgatct cttcgcccgg attatacttg acactgccgt
    12961 tttcaaaggg ctcgaaagcc ccgcgcatga gaaatcccgc ccccaggccg gcgaccatgc
    13021 cgccgagcgg aaaggcggaa accagtccgg cgctgacatc cagaccctgg ccgaggatgg
    13081 gaacagtgaa atcaaaggcg tgcagggcga ggatattggc cacgctcagc tcttcgccgt
    13141 tgagggcgtg cttgccgctg ggaaggttga tcccgaaggt ggcaagcaga cgctcatttt
    13201 tgaagagata gctgccgccg aagcgggtgt cggagaatcc gttcagggcg gcctcgcccg
    13261 agccctcgaa ggtgctgaag gcaggcccgg ccccgatggt aagctgaagc tgctcgttga
    13321 ccggatagag aaaggtgagt ggaatggcga tctggcggac ctgctcctcc tcaacctgcc
    13381 agagattcag gacagcggaa gccgaggcgg tcccctcgcg ggcgcgctga agcatggcag
    13441 gctgggcgaa gagggccgga caggccgcga ggatcagggc ggtgaggagg cgaagtggtg
    13501 ctttgcgttg ctcgatcata gggctttcct tgctgcggat catggctgcg ggttacggtt
    13561 tgacgggcaa ctggatgcgc acctccagcg ggatgccggt gccgatgccg atggtggagg
    13621 attcagtggt gggcttgcgc gactccaccc cggggacaaa accgggggtg atctgggctg
    13681 ctgcgcggct gagcaggtcg atggtgatca aatcacccgc gctggctgac gggaccgctg
    13741 cagcgccgcc ggagggcagg cgcgtgctct cccgccggtc atccctcccg cccgcttcgg
    13801 ccagcagcga cggcggagcc gtctccggcc tgggcgagaa agaggcgaag gccgcgctgc
    13861 gcccgatggc gtcgatggcg gactgaaaag cgggatcggc gctgaccgcg gcccggaact
    13921 gctcctgggc gccctgccag ttgccgcggt cctcttcatc gagtccacgg caccaggcca
    13981 tgaaggcggt cagatttttg gtcgggatgc gctggatcgc ctcccgctcg ccgcgggaaa
    14041 ggcgcagccc catgacgtcg atgagattga agaccagatc cttctggagc tggtaaaact
    14101 cctccaggga gcccgagatc ttttccgccg cggcgggagt ctccaccccc accgtcgcca
    14161 gcagtgcatc gattttcagc attttggcat ccgcgccgga aaaagtgccc tggacgattc
    14221 ggccggcctt gagaagccgg cccatgcgcg gggcggtctc ctccttgacc aggccggtca
    14281 tgcccagccc catctcatcg agcagcatct gcatgcgcgc acgctccacc acctgcaaac
    14341 tcttgacctg ggcgagatcg gtgatcagca tctccgccag ccccttgcgc agggggacga
    14401 aatcgggatt gccggaaaga tcggtgaaat agaggacggc gattgaattt tcaggcgccg
    14461 gggaggcctc gagcgcctcc tcctgctcga gcagggcctt gatctgggcg gccatctgct
    14521 ggcggacgag aacctgcatg cgtccttcga cagtctggcg catcttgcgt gcgggattga
    14581 gctgggtata gcggctgtag tgctggaggg cctga
//
LOCUS       DAOPWU010000045        14481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4263_length_14481_cov_5.222168, whole genome shotgun sequence.
ACCESSION   DAOPWU010000045 DAOPWU010000000
VERSION     DAOPWU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4263_length_14481_cov_5.222168"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..9939
                     /locus_tag="PKM23_04180"
     CDS             55..9939
                     /locus_tag="PKM23_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90701.1"
                     /translation="MPGSATRLAISGEPLTIAAEAAFPNDITLRAFDKHGNPATSYAG
                     TITFANDDPAYQAGDLPPAALMTGVQKIYPKSDFKLRTAGARRIWASDGTLADTSGVI
                     NVTGGSISNYNISLSASPVAGQPVTVTVNNATDGAGNPASGNVTLTFADGGSHSIGTY
                     TPSLPVITVWNGSGSGTATFFKAEGPLALKGLSGSIERTSNEFTVQPGPLGELIFIAD
                     PGTLTPLPESVTAGDSLGGALEYLLVTLRDGYGNVKTDFNGTVSFTSSDGLATHPDAY
                     PFTSGDQGSKKFARDQFKLETAGNHTFTAQTGTFSRKLNPILVYSDVIATFTMTAGSN
                     QAAGVPFIVTVSNARDRFNNPASGLVEIIALVGGNLAPNGQRPTFTPIPVNNGSGQAA
                     QILVMTETVQLRGQVLAGGSVLASRDTPDIAVNPGALGHLEFSGLPASVQAGAPFPGD
                     VTVVAYDAYRNLKTDYEGTIRFNSSAAEVTLPPDKKFDKYAQYSFPAASFSITTPGLQ
                     TITVRDRDASPVVSTTSAPIDVSALRITAMTTAPVRISQGQTGLLVQMELENIGSSEV
                     TDLSTGLHFGPGRDGDYTYTYSWPAGLPIIPANSRRTVDFLVDARTTAQTGSIRISGF
                     ASGIYNGAPVSVDDAARFDTWLVQQKAALSLTSVTIAADTVNQGQSGILIECVVRNGS
                     STALADARISTAAFTFRLNDTNNVSASFPVTAALTNDTLIAATQSRTLRFYLQSTLTA
                     PVGRIHLYPIIRYRDVNSGIIDSTLFSLNDTFFSQEAGALEITEITPSQPTVTRGQAR
                     AWTVTVGVRNTASVPLEIDFNTSKTYLEFRRAGIDYSANFTILNPPTILSNNSYVLAK
                     NGATGDSSGIVYTINAVNAPPGTYSIVSRVETTDGIRASDAFGSVEVQTDADLFISKI
                     SPSQAYATVGDATWPWYIDVSLTNQGGSTVEIDDINSRLDFTTPGFILGPPRLVQNGT
                     ILRGNQSDILRFPVLRNGNAPAAVTISATINYQVLNSGAPRTLVSPVTGTVRLQNKAA
                     NFVQKIRLDPGILTEDKLAPWRVVLTLASSATDGDLQINLDDADSTFVRFLDYQLPLP
                     ARLAGNGTRVLKSGTTDSLIFTGIVPVDTSGSFAITARVAARDLNSSLAVSAAPPQSV
                     ALVVQNPPSLAALTNSLQPAYVSGGTVYQFQINVVNNGGSTLLLDPTRTAFAIVDTTT
                     GLELYSSLLDASFGSSLPGNMSSRTLYFHFKDLPAGIAPGRYRTRLLLAGTENRNAFS
                     QTLELSNNRVTVASPREVMITSLRSESPTVTAGQGLPWFIALEVTNNGSSTLRLDQSS
                     ITFYRNNVDVSSSFALVVPDTFTTGLATIPGDTTRIIRYRVDAVDADLEPGQIVIAGH
                     IWLTDVAQAYRRFDEQTDQGNSGYVTVQAPAEALLISFSPSQNSVTRGQQEPWTISAR
                     VKNRGGSILALDPARLELHFSAGDTHFVFQQPSHFLGSGDTLLLPGVEDTLRWTVDEV
                     SAESSLPGLVRVEAAIPLSELNSGRLYTLETSSGNAGFDIAVQDSAAARLAGFRVVVP
                     QDSLVNAGQSFYVQACVKSLENRDGLENVRVRFAADGYLSFPEGSETVLGTIPAGDSL
                     WTVPGILVKADTMAGVDPTLFARILSAAAANTGQPTLIEPARAGRDSSRTIHIQKPGA
                     LTVTRIFVSQDTIPSGYSLDWTIQVDVTNPREGTLLLEAPRAGDISIKQGFVIRAPEL
                     TPAERTLSPGDTLRVIYEVIASSSGSGRLPITAQLAARDVNDTLRVVSTSATTSIFVS
                     TSARVRIVRTAVNADSNFVDAEGIGHVNTRQPFYVDVDVENSGGQPLKTVYVRLAGVR
                     SPVATPLQFIENLSTFEGPRRLRFSVQADSLENLAGELFTAEITQAAGEDGSPAFIAP
                     ATDALATARIYRPAVLQLVSTSALNPGTERIVSYGQAFPVEVVVQNLGSEPVSGVTLT
                     MRADPADRAGITTTPLLLAKTLAAGDTGKVVFTATAGSQSGEVLLVSDVTTSTGQNTG
                     LPVPLQRAGQDSSSLVTIESGARLTILSVRVVPAAVSAGDAQNDTRIYVQVANTGDAD
                     LRFLGISPDNVRFTTNGILDEEYRVNAPAKLMGSDSLVLKGQAQDSLLYIVTRIGDIA
                     GDAEVRVQLTAMDLNRSPSEANQMMATGTTSLEVTSTSWVRINQTLVSSATAFDEQNT
                     PLVNRGRQFILAVEAETSELSGVDSVWVRLTAEGASSIVQPLLVIPTINKGGTGRAEF
                     TIIADDSWDGALGEIRESFNAQILSAKAVGSSLAAQVRAPVRPVDALATVRIQNPARI
                     DLRLALAAGQDSILTASQEFGLVVSLVNLGSSAMRDGQYTITLPPGNKYRLIGGETER
                     SFDVPLGWTSYTDTLHLIAPDQDSFSDTIRVNLTRVPQDVNIAAPAATESVQAAAVVT
                     TLRSGLALNLSIYSPSGAQDRILSTAQQMVLRATVQSTDNIRNKSVTLKLPISPAYVL
                     LSPASQAVASSRDTVYWRVQVPDAETVMAHIFEATARGQSAESELIQTRTATIDQIVS
                     RASLWVDDLEVSSPAVGVMESGQANFSVRQQAILRTRVRNLGAARVGATGRITLSLQN
                     SGLTLVSSDSAKGFTIGSYVTWNVQASDVPISEIRDIRVEISTIPMDENTNAPAAIAN
                     GSATLNVLTEARGTTRIDQFYISSPAGALDNSVSAEQSFVVTAEITSTSVRELQAEIS
                     STGDFSTNTPSVTVPAGNRQVVTWTVKAPAEAGSNTLTLTVSGRDYRSNIALTNQVRA
                     LNVTTQPPTRFTLTPRIIFPAGLSNKVSSESPMRLALYIDHREETAPQVAGEAATIRL
                     NVPARFLDGSEPLIKSGSDSLVWNLAAPAVSQDTLFDVQFSVTALPKDANSGLEATTE
                     FMNVYFPIWVVRKARLEVLAQVQGRSGTTPIPVRIGNEFDLFSILSNLGSADYYGSYQ
                     VQLRLPSGYTSADGLTVSTESDTVKWRVKAPDVFRETPDTLMVKLLSAPRDYFSKTPA
                     DIAQDSAIVLISPEAGFMVAKSFPVRTGSVGLRGGASLPMLGLSLQNKDQSIGSRSLL
                     DTIKVSFRNKRGSAVAARSVVSRIAAVRHGNPAEVLAENNSPGAASEMVLNFDKAVPD
                     TIRGSDLFAIDILIDIAAEANLTDFVMAVDSARAIVARDAIYHNRLSIADSTLNRVQY
                     LGFSSGTLVVMQNNLEESFCNYPNPFGTASRPTTRFVYYLKQASDVRIKIFTLTGDLV
                     QAWEYTRAAHPRQTSAGVHQDEVIWDGRNGRGEKVMNGVYLAYLQTDYSEMALTKIAV
                     VK"
     gene            9961..11949
                     /locus_tag="PKM23_04185"
     CDS             9961..11949
                     /locus_tag="PKM23_04185"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY90702.1"
                     /translation="MKPNTRKYLLSLLFLLLLQAGGAYAQQVLQDRAYGGANDVFDLP
                     VGARALAMGGAYVSTADDPFALYWNPAALENVQNMGVGLYYSNLTAGTQYSYLGYAHP
                     TLFLGTFSLGIISLSTPDIDIRDEFDPMKLGSLSYSRNMLLFGYGYKPLSWLAVGAAL
                     KVERVSLPGYPDGATGQTGSLTESALGADMGLVFTPGLASPYLKDLTLAVSVQNAIQR
                     SMRAVDERESTPRTFRLGMDKRISIGDDGSSVTLAFEYDKMKTMPAHTHLGLEYSFQD
                     MFALRTGLYRNHFTIGGGAKLAGVQLDYSYWTGDDSWLSSSHRISLVLNIGKSRELRM
                     AEYQEREARRIEMELAAKRQAERNEAIVSGMSRARVLVGKGDYPGAYSVVSRVLFYDV
                     TGSDPDLEEARQLLQQINTALEEQRKREEAAILAKTEEEMRIRRNNLLIEQHYQKALN
                     AYSLEDYQTAIQECDQALAIDPNSERATDLRRKSDEQLRRKIMQLAERARQLQAQDRG
                     YDAITLYNQARQMAKGIPDIETFLAGQISSIESRLSREDLMRRAAAQEMSQNWAEAAS
                     LYREALRYDPNNRSLQERYNEAKARAEARDMEMPENVRELYRLGAQAYAQHKYDEAVR
                     HFEEARKLQPLNKKILKALDSAKENLQRQSSNQGTQNK"
     gene            12029..>14481
                     /locus_tag="PKM23_04190"
     CDS             12029..>14481
                     /locus_tag="PKM23_04190"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNY90703.1"
                     /translation="MFQNILKEQLKVPARIEFLGELRDFVTKVGHRHGFSERVINAFK
                     LSIDEAATNIIKHAYRDWEGSITIRAIVKKNSLTIVLVDQGKYFDPRQVSDPDLQRYV
                     DIGKKGGLGIFIMRRLLDEIDYRKTEEGNELWMVKHRDVARKRRLAVPSLSMTLKARY
                     WLISTSVFTLFLLGVFVYNFFRSADSITADFLARGRTALSVMTSDISANLQQQLPETT
                     RQSMVEGTNLPDDIDILAHTLPPLNTLQNSEYRDILYQALIADNNQYILASTEGKLIF
                     TRFNRPQETGKLQNDVLRYTFPSGIEVLEITSPVLDESGKTLCTAHFFIDHAAISKEI
                     SSERWSNARMLGLIWLIGAAGLFLLIILVMNPFRRLSEWVKQLGQPGAADEMDIDATT
                     EIGEIAQAFSDITLKLRESQVNMAEQERLQKEMQVAQEIQQTLLPGEFPEIEGYGISS
                     YYAAAKEVGGDYYDFVEVDKDTLGIVVADVSGKGVPGSLVMTMIRTALRTEARGVKDA
                     AEVLARVNDFVVADMKKGMFVTLFYVIIDSKKRRLNYASAGHNPMILYRGSRNKSYYL
                     NPHGFPIGISLPDKNLFRNTIESDTLSLNEGDILLIYTDGITEAMNRRRQLYGEERFL
                     NVIRENGLLPAQDFVDKLHEEILSFTEGTPQSDDITLVAIKEKSSAEKIELERAKRVY
                     RRLQQGSSIKEACESEGLSSYSYYSKYKEIFEQGNIEELVSVESESIEAKHLSIEEKT
                     RLYDVIMRYPEYGAKKIHEELLTERYGFMDIHPAKIYEELVRAKLNTRELREAFVHRG
                     GKKRRPKPPGTPMLTLDGRV"
BASE COUNT         2881 a         4727 c         4132 g         2741 t
ORIGIN      
        1 cttcgaggaa cctcagcggc tgcagcgatc caaaccacca atcccttcaa tatcttgccg
       61 ggcagcgcca cccgcctcgc catcagcggc gaacccctga ccatcgccgc ggaagcggcc
      121 tttcccaatg acatcaccct ccgggccttc gacaagcatg gcaatccagc cacgagttat
      181 gcgggtacga tcactttcgc caatgacgac cccgcctatc aggcggggga tttgccgccg
      241 gcggccctga tgaccggagt gcaaaagatc tatcccaaga gtgatttcaa gctgcgcacg
      301 gcgggcgcgc gcaggatctg ggcttcggac ggcacccttg ccgacacctc gggcgtcatc
      361 aacgtaaccg gcggctctat cagtaattat aatatttctc tttcagcctc acccgtcgcc
      421 ggccagccgg tgacggtcac agtcaacaac gcgaccgatg gcgccggcaa tcccgccagt
      481 ggaaatgtca ccctcacctt tgccgatgga ggatcccatt ccatcggcac ttatacacca
      541 agcctgccgg tgatcaccgt ttggaacggt tccggaagcg gcaccgccac ctttttcaag
      601 gccgaggggc ctctcgccct gaagggactc tccggaagca tagaaagaac atcgaacgaa
      661 ttcaccgttc agcccggccc cctgggcgaa ctcattttca tcgccgatcc gggcaccttg
      721 acgcccttgc ccgaatccgt caccgccggc gactcgctcg gcggcgccct cgaatatctc
      781 ctggttaccc tccgggacgg ctatggcaac gttaaaaccg atttcaacgg taccgtcagc
      841 tttaccagca gtgatggctt ggccacacac cccgatgcat acccattcac gagcggggat
      901 caggggagta aaaagtttgc ccgggatcaa ttcaagctag aaacagccgg caaccatacc
      961 ttcacggccc agaccggaac cttttccaga aagctcaatc ccatcctggt ttacagcgat
     1021 gtcatcgcca cttttacgat gacggctggc agcaaccagg cggccggggt accctttatc
     1081 gtcacagtca gcaatgcccg ggatcgcttt aacaatccgg cctccgggct ggtggagatc
     1141 atcgccctgg tgggcggcaa cctcgccccc aacggccagc gcccgacctt cacccccatc
     1201 cccgtcaaca acggcagcgg ccaggcagcg cagatactgg tcatgaccga aacggtccag
     1261 ctgcgcggcc aggtcctcgc gggcggatcg gttctggcct ccagggacac cccggacatt
     1321 gcggttaatc cgggtgcgct cggtcatctg gagttcagtg gcctgcccgc atcagtccag
     1381 gcgggtgctc catttcccgg cgacgtgacg gtggtcgcct atgatgccta tcgtaacctg
     1441 aaaactgact atgaaggcac catccggttc aacagcagcg ctgcagaagt gaccctcccg
     1501 ccggataaaa aattcgacaa gtatgcgcag tactccttcc ctgcggcgtc cttcagcatc
     1561 acgacgcccg gcctgcagac catcacagtg agagatcggg acgcctctcc ggtggtctcc
     1621 accaccagcg cccccatcga tgtcagcgcc ctgcgcatca cagcgatgac aacggcgcca
     1681 gtccggatta gccaggggca aacgggcctt cttgtgcaga tggagctgga gaacatcggt
     1741 tcttccgagg tcaccgatct cagcacaggt ttgcatttcg gacccggccg cgacggggat
     1801 tacacctaca cctattcctg gccggcagga ctgccgataa tccccgccaa ttcgcggcgg
     1861 acggtagatt ttctggtgga cgcccggacc actgctcaga ccgggtcgat ccggatcagc
     1921 ggatttgcaa gcgggatcta caacggggcg ccggtcagcg tggatgacgc tgccagattc
     1981 gacacctggc tggtccagca aaaagccgct ctcagcctga ccagcgtcac catcgcggcg
     2041 gatacggtga accagggcca gagcggcatc ctgatcgagt gcgtggtcag gaatggctct
     2101 tccacagccc tggccgatgc ccgcatcagc acagccgcct ttacgtttcg tctcaatgat
     2161 accaacaacg tctccgcctc tttcccggtc accgcggcgc tgaccaatga caccctgatc
     2221 gcggcgacgc agagcaggac cctgcgtttt tatctgcagt caacccttac ggctccggtc
     2281 ggcaggattc atctctaccc tatcattcgc taccgggacg tcaattccgg gatcatcgac
     2341 tcgaccctct tctccctgaa cgacaccttc ttcagccagg aggccggtgc gctcgaaatc
     2401 accgagatca cgccttcgca gcccaccgtc acccgcgggc aggcgcgcgc ctggacggtc
     2461 actgtcggcg tcagaaacac cgcctcggtt ccgctcgaga tcgatttcaa tacgagcaaa
     2521 acctatctcg aattccgccg ggccggaatc gattattcag cgaactttac tattctcaat
     2581 cctccgacta tcctctccaa taactcgtac gttttggcca agaacggggc caccggcgac
     2641 agcagcggga tcgtctatac catcaatgcc gtcaacgccc cgccgggaac ctattccatt
     2701 gtcagccggg ttgagactac cgatggcatc cgcgccagcg atgccttcgg cagcgtcgag
     2761 gtacagacgg atgcggatct cttcatctcc aagatctccc cttctcaggc ctacgcgacg
     2821 gtgggcgatg ccacctggcc ctggtacatc gatgtcagct tgaccaatca ggggggcagc
     2881 acagtcgaga tcgatgacat caacagccgg ctcgatttta cgacaccggg gttcattctc
     2941 ggaccacccc ggctggttca aaacggcacc atactccgcg gcaaccagtc ggatatcctg
     3001 cgcttcccgg tcctcaggaa tggaaatgcg cctgctgcgg tgaccatcag cgcgactatt
     3061 aattatcagg ttctcaactc cggcgctcct cgtaccctgg tctcgccggt caccggcacc
     3121 gtccgcctgc aaaacaaggc ggccaatttt gtccaaaaga tccgcctcga tccgggcatc
     3181 ctcaccgagg acaagctcgc cccgtggcgc gtcgttctca ccctggccag cagtgcgacc
     3241 gatggcgacc tccagatcaa tctcgacgac gccgacagca ccttcgtccg ctttctggat
     3301 tatcagctcc cgctgccggc ccgcctggcg ggcaacggca cccgcgtgct caagtccggc
     3361 accacggaca gcctgatatt caccggcatc gtccccgtcg acaccagcgg atcttttgcc
     3421 atcacggcac gggtagctgc ccgggacctc aacagcagcc tcgccgtcag cgctgcaccg
     3481 ccgcagtcgg ttgccctcgt ggtacagaat cccccgagcc tcgcagccct gaccaattcc
     3541 ctgcagcccg cctatgtttc gggaggtaca gtctaccagt ttcagattaa cgtcgtcaat
     3601 aacggtggat caaccctgct cctggatccc acccggactg ctttcgcgat cgtggacacc
     3661 acgaccggcc tggagctcta ttcctccctg ctcgacgcct ctttcggatc aagcctgccg
     3721 ggcaacatga gctcgcgcac gctctatttc catttcaagg atcttcccgc cggcatcgcc
     3781 ccgggacgct atcgcacccg cctcctgctc gccggcacgg aaaaccgcaa cgccttcagc
     3841 cagaccctgg agctgagcaa taacagggtc acggtggcga gcccgcgtga ggtcatgatc
     3901 acttccctgc gcagcgagtc gcccaccgtc acagcggggc agggactgcc ctggttcatt
     3961 gcactggagg tgaccaacaa cggcagcagc accctgcgcc tcgaccagtc cagcatcacc
     4021 ttttatcgca acaatgtcga tgtgtcctcc tccttcgccc tggtcgtgcc ggataccttc
     4081 accacgggcc tggcgaccat tccaggcgac accacccgga tcatccgcta ccgggtcgat
     4141 gcggtcgatg cggatctcga gcccggccag attgtcatcg cgggccatat ctggctcacg
     4201 gatgtagccc aggcctatcg ccgtttcgat gagcagaccg atcagggcaa ttccggttat
     4261 gtcaccgtgc aggccccggc ggaagccctt ttgatctcct tctcgcccag ccagaattct
     4321 gtgaccaggg gccagcagga gccctggacg atctccgcgc gggtgaaaaa tcggggtgga
     4381 agcatcctcg ccctcgatcc cgcccggctg gagctccact tcagcgcagg agacacccac
     4441 ttcgtcttcc agcagcccag ccatttcctc ggcagcggcg atacccttct gctgccgggc
     4501 gttgaggata ccctgcgctg gactgtggat gaggtctcgg cggagtcgag cctgcctggc
     4561 ctggtgcggg tggaggcggc gatacccctg agcgaactca acagcggccg tctctatacc
     4621 cttgagacct cctccggcaa tgccggcttt gatattgcgg tgcaggattc tgccgcagcg
     4681 cggctcgccg gctttagagt ggtggtgccg caggactctc tggttaatgc cggacagagc
     4741 ttttacgttc aggcctgcgt gaaaagcctt gaaaatcggg acgggctgga aaatgtgcgc
     4801 gtgcgcttcg ccgccgacgg atatctctcc ttccctgaag gcagcgagac cgtgctcgga
     4861 accatccccg ccggcgacag cctctggact gtgcccggga tcctcgtcaa ggccgatacc
     4921 atggcgggcg tcgatccgac cctttttgcc cggatcctct ccgctgcggc ggccaatacc
     4981 ggccagccga ccctcattga accagcccgc gccgggaggg attcctccag gaccatccac
     5041 atccagaagc cgggcgccct gacggtgacg cggatcttcg tctcccagga caccatcccc
     5101 tccgggtaca gcctggactg gaccatccag gtggatgtga ccaatccccg tgagggaacc
     5161 ctgctgctgg aagcacccag ggcgggcgat atttcgatca agcagggctt cgtcatccgc
     5221 gccccggaac tgactccggc cgaacgtacc ctctcccccg gagataccct ccgggtcatc
     5281 tatgaggtca ttgcctccag ttccggcagc ggccggctgc cgatcacggc ccagctcgcg
     5341 gccagagatg tcaacgacac cctgcgcgtg gtctccacct cggccacaac ctcgatcttt
     5401 gtctccacca gcgcgcgcgt gcgcatcgtc cggaccgcgg tcaacgccga ctccaacttt
     5461 gtcgatgccg aagggatcgg gcatgtcaat acccgccagc ccttttatgt tgatgtcgat
     5521 gtagaaaaca gcggcggaca gccgcttaaa acagtctatg tacggcttgc cggcgtccgt
     5581 tcgccggtcg ccacccccct tcagttcatc gaaaatctga gcacctttga gggcccccgc
     5641 cgcctgagat tcagcgtgca ggcggacagt ctcgaaaacc tggccggcga acttttcacc
     5701 gccgaaatca cccaggccgc cggcgaagac ggctctcctg ccttcatcgc tcccgccaca
     5761 gatgccctgg cgacggcccg catctaccga ccggcggtgc tgcagctggt cagtacttcg
     5821 gcccttaatc cgggcactga aaggatcgtc agttacggac aggccttccc ggtggaggtc
     5881 gtcgtgcaga atctgggttc ggagccggtc agcggcgtga ccctgacgat gcgggccgat
     5941 ccggccgacc gggccggcat cacgaccacg cccctgctgt tagccaagac actcgccgcc
     6001 ggagataccg gcaaggtcgt tttcacagcc acggcgggca gtcagagtgg agaggtgctt
     6061 ctggtttcgg atgtcaccac ctcaaccggc cagaacaccg ggctgcccgt gcccttgcag
     6121 cgcgccgggc aggactcttc gagcctggtg acgatcgaat cgggcgcacg gctgactatt
     6181 ctctcggtgc gggtggtgcc cgccgccgtt tcggccggcg atgcccagaa cgatacccgc
     6241 atctatgtac aagtcgccaa taccggcgat gcggatctgc gctttcttgg catctcgccc
     6301 gataatgtcc gctttacgac caacggcata ctggatgaag agtaccgcgt taacgccccc
     6361 gccaaactga tgggctccga cagcctggtt ttgaaaggcc aggcgcagga ctcgctcctt
     6421 tatatcgtca cccggatcgg ggacatcgcc ggggatgccg aggtcagggt gcagctcact
     6481 gcgatggatc tgaacaggag cccctcagaa gcgaatcaga tgatggccac cggcaccacc
     6541 agcctggagg tgaccagcac ctcctgggtg cgcatcaacc agaccctggt gagttctgcc
     6601 acagccttcg atgagcaaaa tactcccctg gtcaaccgtg gccgccagtt catcctggcg
     6661 gtggaggcgg agaccagcga actctccggt gtggattcag tctgggttcg tctcacagcg
     6721 gagggggctt ccagcatcgt ccagcccctg ctggtcatcc cgaccatcaa caagggcggc
     6781 acgggccggg ccgagttcac tatcatcgct gacgacagct gggatggtgc gctgggtgaa
     6841 atccgcgaga gtttcaacgc ccagatcctt tccgccaagg cggtgggctc ctccctggct
     6901 gcacaggtcc gggcgccggt ccggccggtg gacgcactgg cgacggtgcg catccagaat
     6961 ccggcgcgca tcgacctccg tctcgccctt gcggccggcc aggattccat cctgacggcg
     7021 agtcaggagt tcggcctggt ggtcagtctg gtcaacctcg gctcctctgc catgcgcgac
     7081 gggcagtaca cgatcaccct gccgccggga aacaagtacc gccttatcgg gggcgaaacg
     7141 gaacgctcct tcgatgtacc gctcggctgg acgagctata ccgacaccct ccacctgatc
     7201 gcgcctgacc aggattcctt cagcgacacc atccgtgtca acctcactcg ggtgccgcag
     7261 gatgtcaata tcgcagcacc cgctgcgacc gagtctgtgc aggcggctgc agtcgtcacg
     7321 accctgaggt cgggactggc attgaatctc tccatctatt caccctccgg tgcccaggac
     7381 cggattctct ccacggccca gcagatggtg ctgcgcgcga ccgtccagtc gaccgacaac
     7441 attcgaaaca agagcgtcac cctcaaactc ccgatctccc ctgcctatgt tctgcttagc
     7501 cctgcgtcgc aggcggtggc ctccagccgg gatacggtct actggcgcgt ccaggttcct
     7561 gatgccgaga ccgtaatggc ccatattttc gaggccacgg cgcgcgggca gtccgcggag
     7621 agcgaactga tccagacccg taccgcaact atcgatcaga tcgtaagccg cgccagcctc
     7681 tgggtcgatg accttgaggt gagttccccg gcagtagggg tgatggagag cggccaggcc
     7741 aatttctccg tacgccagca ggccatcctg agaaccaggg tgcgcaatct cggcgccgcc
     7801 cgcgtcgggg cgactggcag gatcaccctc tccctgcaga acagcggttt gacgctggtc
     7861 tcctctgatt cagccaaggg cttcaccatc ggcagctatg taacctggaa cgtccaggct
     7921 tccgatgtgc ccatcagcga gatccgggat atccgtgtcg agatctccac catacccatg
     7981 gacgagaata ccaatgcccc ggcggctatc gccaatggtt cggcgacgct gaatgtcctc
     8041 accgaggcgc gggggacaac gcgcatcgat cagttctata tcagcagccc ggctggcgcc
     8101 cttgacaatt cggtctctgc ggaacaaagc tttgtggtga cggcggagat cacctccacc
     8161 tcggtccgtg aactgcaggc cgagatcagc agcaccgggg atttttcgac caatacaccg
     8221 tcggttacgg tcccggccgg gaaccgtcag gtggtgacct ggacggtaaa ggccccggcg
     8281 gaggccggat ccaacaccct gaccctgacg gtgagcggca gggattaccg ttcgaacatt
     8341 gccctgacca accaggtccg cgccctcaat gtgaccaccc agccgccgac ccgtttcacc
     8401 ctgactccgc gcatcatctt cccggcgggg ctgagcaaca aggtctccag tgaatcgccg
     8461 atgcggctgg ccctctacat cgatcatcgt gaggagaccg ccccccaggt ggcgggcgaa
     8521 gcggctacca ttcgtctcaa tgtccccgcc cgctttctcg acggcagcga acccctgatc
     8581 aagagcggca gtgacagcct ggtctggaac ctggccgccc cggcggtttc gcaggacact
     8641 ctttttgatg tccagttcag cgtcacagct ctgccgaagg atgccaattc cggtctggag
     8701 gcgacgacgg agtttatgaa tgtctatttc ccgatctggg tcgtgcgcaa agcgcggctt
     8761 gaagtgctcg cccaggtaca gggccggtcg gggacgacgc caataccggt gcgcatcggc
     8821 aatgagttcg acctcttttc gatcctctcc aatctcggct ctgcggatta ctacggttcc
     8881 taccaggtgc agctgcggct gccctcgggc tatacgagcg cggacggcct gaccgtaagc
     8941 accgagagcg acacggtgaa gtggcgggtc aaggcgccgg atgtattcag ggagaccccg
     9001 gataccctga tggtaaagct gctcagtgcg ccgagggatt atttctccaa aacccctgct
     9061 gatatcgcgc aggattcggc gattgtgctg atctcgcctg aggctggttt tatggtggcc
     9121 aagtcctttc ctgtgcgcac cggatcggtc ggcctgcgcg gcggtgccag cctgcccatg
     9181 ctcggcctct ctctgcagaa caaggaccag tcgatcggca gccggtcgct gctggatacc
     9241 atcaaggtct ctttccgcaa caagcgcggc agcgcggtgg cggcgcggtc ggtggtgagc
     9301 cggatcgccg ccgtccggca cggcaatccc gctgaggtgc tggctgagaa caatagtccc
     9361 ggcgcggcca gcgagatggt gctcaatttt gacaaggctg ttcccgacac gatccggggc
     9421 agcgacctct tcgccatcga tatccttatc gatatcgctg cggaggccaa cctcaccgat
     9481 tttgtcatgg cggtggattc ggcccgtgcg atagtagcca gggacgccat ctaccacaac
     9541 cggctgagca tcgccgactc gaccctcaat cgcgtccagt atctcggctt ctcctccggc
     9601 accctggtgg tgatgcaaaa caacctggaa gagtcttttt gtaattatcc caatcccttc
     9661 ggcacagcgt cgcgcccgac cacccgcttt gtctattatc tcaagcaggc gagcgacgtc
     9721 cgtatcaaga tcttcaccct gaccggagat ctggtacagg cctgggagta caccagggcg
     9781 gctcatccgc ggcagaccag cgccggagtt catcaggatg aggtcatctg ggacggccgc
     9841 aacggccgcg gcgagaaggt gatgaacggc gtctatctgg cctatctgca gaccgattac
     9901 agcgagatgg cgctcaccaa gatagcagtt gtcaaataac gcatcgagca gttggccatt
     9961 gtgaaaccaa ataccagaaa atatctcctc tctttgctct tcctgctgct tcttcaggcc
    10021 ggaggggcgt acgcgcagca ggtgctgcag gatcgagcct atggcggagc caatgatgtc
    10081 tttgaccttc ccgtcggggc ccgggctctg gccatgggcg gggcctatgt ctcgacggcc
    10141 gatgatcctt tcgccctata ctggaatcca gccgccctgg agaatgttca gaatatgggc
    10201 gtcgggctct actattccaa tctgaccgcg ggcacacagt acagctatct cggctatgct
    10261 caccccaccc tttttctggg caccttcagc ctcggcatca tcagtctgtc gacgccggac
    10321 attgatatcc gcgacgagtt cgatccgatg aagttgggct cgctctccta cagccggaac
    10381 atgctgctct tcggctacgg ctacaagcct ctcagctggc ttgctgtcgg ggccgcgctc
    10441 aaggtcgagc gggtttcgct gccgggctat ccggatgggg ccacggggca gaccggcagc
    10501 ctcaccgaat ccgccttggg cgcggacatg gggctggtct tcactcccgg gctggcgagc
    10561 ccctatctga aggatctcac cctggctgtg agcgtgcaga atgcgattca gcgctccatg
    10621 cgcgcggttg atgagcgtga atccacgccc cgcaccttcc gtctggggat ggacaagagg
    10681 atcagcatcg gtgatgacgg cagcagtgtg acgctggcct ttgagtatga caagatgaag
    10741 acgatgcccg cccacaccca tctgggattg gagtacagct ttcaggatat gttcgctctg
    10801 cgtacggggc tttatcgcaa tcactttacg atcgggggcg gcgccaagct ggccggtgtc
    10861 cagcttgatt actcttactg gacgggcgat gattcctggc tgagcagcag ccatcgcatc
    10921 tccctggtgc tgaacatcgg caagagccgc gaattgcgca tggctgaata tcaggagcgt
    10981 gaggcgcgcc gcatcgagat ggagctggcg gccaaacgcc aggctgaacg gaacgaggcc
    11041 attgtttcgg gcatgagccg tgcgcgggtc ctggtcggaa agggtgatta ccccggggct
    11101 tacagcgtgg tgagccgggt gctcttttac gatgtgaccg gcagcgatcc ggatctggag
    11161 gaggcgcggc agcttttgca gcagatcaac accgctttgg aggagcagcg caagcgcgag
    11221 gaggcggcca ttctcgccaa gactgaggag gagatgcgca tccgccgcaa caatctgctc
    11281 atcgagcagc attaccagaa ggccctgaac gcctacagcc tcgaggatta tcagaccgcc
    11341 attcaggagt gcgatcaggc cctcgccatc gatccgaaca gcgagcgggc tactgacctg
    11401 agacgcaagt ccgatgaaca gctgcgccgc aagatcatgc agctggccga acgcgcgcgc
    11461 cagctgcagg ctcaggatcg cggctacgat gccatcaccc tctacaatca ggcccggcag
    11521 atggccaagg ggattccgga catcgagacc ttcctggcgg ggcagatctc ttcgatcgaa
    11581 agccggctca gccgggagga tttgatgcgg cgggcggcgg cgcaggagat gagccagaac
    11641 tgggccgagg cggcctcgct ctaccgggaa gccctgcgct acgatcccaa caaccgctcc
    11701 ctgcaggagc gctacaacga agccaaggcc cgggcggaag cgcgcgacat ggagatgccc
    11761 gaaaatgtgc gcgagctcta ccggctcggg gcacaggcct atgcccagca caagtatgat
    11821 gaggcggtgc gccactttga ggaggcgcgc aagctgcagc ccctgaataa aaagatcctc
    11881 aaggccctcg attcagccaa agagaatttg cagcggcaat cttcgaacca gggcacccag
    11941 aacaaataag agcagccggc ccgcaatccg tctgaaagga gctctccgga ccggcggcgc
    12001 ccgccgccgg tgaaggggaa gaggacactt gttccagaat attctaaaag agcagctgaa
    12061 ggtacccgcc cggatcgaat ttctcggcga actgcgggat ttcgtcacca aggttggcca
    12121 ccgccacggt ttctcagagc gggtgatcaa tgccttcaag ctctccatag acgaggcggc
    12181 caccaacatc atcaagcatg cctaccgcga ctgggaaggc agcatcacaa ttcgcgccat
    12241 cgtcaagaaa aacagcctca ccatcgtcct ggttgatcag ggcaagtatt tcgatccgcg
    12301 tcaggtcagc gatcccgacc tccagcgcta tgtcgacatc ggcaagaagg gcggcctggg
    12361 aatcttcatc atgcgccggc tgctcgacga gatcgattat cgcaagaccg aagagggcaa
    12421 cgagttgtgg atggtcaagc atcgcgacgt tgcgcgcaag cgccgcctcg cagtgccctc
    12481 gctctccatg accctcaagg cgcgctactg gctgatatcg acctccgttt tcaccctctt
    12541 tttactcggg gttttcgtct acaacttttt tcgcagtgcc gactctatca ctgccgattt
    12601 tctcgcccgg gggcgcaccg ccctctcggt gatgacctcg gacatatcgg ccaatctgca
    12661 gcagcagctt cccgagacca cgcgccagag catggtggag gggaccaatc ttcccgacga
    12721 cattgatatc ctcgcccaca ccctgccgcc gctcaacacc ctgcagaatt cggaataccg
    12781 ggatatcctc tatcaggcgc ttatcgccga caataaccag tacattctgg ccagcaccga
    12841 gggcaagctg attttcaccc gtttcaatcg gccgcaggaa accggcaagc tgcagaatga
    12901 tgtcctgcgt tacaccttcc cttcagggat cgaggtgctg gaaattacct ccccggttct
    12961 ggatgagtcc ggcaaaaccc tttgcaccgc ccactttttc atcgatcacg ctgccatcag
    13021 caaggagatc agcagcgagc gctggagcaa cgcccggatg ctgggcctga tctggctcat
    13081 cggcgccgcc ggtctctttt tgctcatcat cctagtgatg aaccccttcc ggcgtctctc
    13141 cgagtgggtc aaacagctcg gacagcccgg tgccgctgac gagatggaca tcgatgccac
    13201 aaccgagatc ggcgagatcg cccaggcctt cagtgatatc accctgaagc tgcgggaatc
    13261 ccaggtcaat atggccgaac aggaacggct gcaaaaggag atgcaggtcg cccaggaaat
    13321 ccagcagacc ctgctgccgg gcgaatttcc cgagatcgag ggctacggaa tcagcagcta
    13381 ttacgccgcc gccaaggagg tgggcggcga ctattatgat ttcgtcgagg tggacaagga
    13441 taccctgggc atcgtcgtcg ccgatgtttc gggcaagggg gttccgggtt cactggtcat
    13501 gaccatgatc cgcaccgccc tgcgcaccga ggcgcgcggc gtcaaggatg cggccgaggt
    13561 gctggcgcgc gtgaacgact ttgtcgtcgc agacatgaaa aaggggatgt tcgtcaccct
    13621 tttttatgtg atcatcgatt ccaagaaaag gcggctcaac tatgccagcg ccggacacaa
    13681 tccgatgatc ctctatcgcg gcagccgcaa caaatcctat tacctcaatc cccacggctt
    13741 ccccattggc atctccctgc cggacaagaa tctcttccgc aacaccattg agtcggacac
    13801 cctctctctc aatgagggcg acatcctgct gatttacacc gacggcatca ccgaggcgat
    13861 gaaccggcgg cgccaactct acggggagga gcgttttctc aacgtcatcc gcgaaaacgg
    13921 ccttctgccg gcccaggatt ttgtcgacaa gctgcacgag gagatcctct cctttaccga
    13981 gggcacaccg cagagcgacg acatcacgct cgtcgccatc aaggaaaagt cttcagcgga
    14041 aaagatcgag ctcgaacgcg ccaagcgggt ctatcgccgt ctccagcagg gcagcagcat
    14101 caaggaggcg tgcgagagcg agggcctttc ctcctactcc tattattcca aatacaagga
    14161 gatctttgag cagggcaaca tcgaggagct ggtcagcgtc gagagcgagt ccatcgaagc
    14221 caagcatctg agcatcgagg agaagacccg tctttatgat gtgatcatgc gctaccccga
    14281 gtacggagcc aagaagatcc atgaggagct cctcaccgag cgctatggct ttatggatat
    14341 ccatcccgcc aagatctacg aagagttggt gcgggccaag ctgaacacgc gcgagctgcg
    14401 cgaggccttt gttcatcgtg gcggtaaaaa gcgccgaccc aagccgccgg gtaccccgat
    14461 gctgacgctg gacgggcggg t
//
LOCUS       DAOPWU010000046        14355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4311_length_14355_cov_5.601469, whole genome shotgun sequence.
ACCESSION   DAOPWU010000046 DAOPWU010000000
VERSION     DAOPWU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14355
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4311_length_14355_cov_5.601469"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1170)
                     /locus_tag="PKM23_04195"
     CDS             complement(163..1170)
                     /locus_tag="PKM23_04195"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNY90704.1"
                     /translation="MHKSIEGELQDLKKRLGPSPITPVFEGLAFIRYHVTAGTGIELI
                     REVGRKTGYRLARAWRQVKAGEVYYELLLDPSLPAMIVYENGRSLEGYPRLDGLAFRV
                     RDLQPVRDSLRNACIPSREEPWGIATDPIPGPGDRFLYLPEGTPAWHEAPGMEPQQIA
                     PESAGTAPFTDVRANIGQLDHIAYRVPLSHVHRTAEMIMKLSGYSFDSCYTVGDQNAE
                     TMVFRWGSRKPAIVVSYGWDEQSVVHAYTAKYGPRVHHTAYYTDDLRTVLQRQQELGL
                     TFTTEELIGDETRGILQIFGTPSPISHEITEYVQRFGSFTGFFDKGNVGELMGSTKGL
                     S"
     gene            1635..2006
                     /locus_tag="PKM23_04200"
     CDS             1635..2006
                     /locus_tag="PKM23_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90705.1"
                     /translation="MLFFLIALGLFAWWRYYFVFGEGVKAGDLNFLVKKGYVFKTWEG
                     RLIQTGFKTPAPGSMQSNEFEFSVVSDEVAKKLENASGKFVELRYKEYLHALPWRGMS
                     RYVVTQVLRIEEQREENDLPY"
     gene            complement(2071..2832)
                     /locus_tag="PKM23_04205"
     CDS             complement(2071..2832)
                     /locus_tag="PKM23_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HNY90706.1"
                     /translation="MLAKEWLENARGVMDRIESTQVENINKAAALMADAIGSGHWVHT
                     FGCGHATLPIEEMYPRIGGFVGFHPMIEIPLSFFTHIVGEMGVHQFVFLERVEGYGQQ
                     IMKSYNFDQNDVMWLFSHSGINNVNIDIALEARRLGMKIVVFGSAGEFSGVKTRHSSG
                     KQIFELADVVVDTCVPAQDASVPLKNHVDKIGPVSTMAFVTAVWMTICTVAEILADRG
                     VKLHIHPSHNVPGDTTARQRLDEALAEYKRRIAGV"
     gene            complement(2836..3999)
                     /locus_tag="PKM23_04210"
     CDS             complement(2836..3999)
                     /locus_tag="PKM23_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY90707.1"
                     /translation="MSYNRKAVFSAACVAMFIFGIVMSILGALLPSVIEQFGLDKAMA
                     GSLFPLMTIGMLLGSLLFGPIVDRYGYKILLIVCSTFVLAGLLMIGRATGLDLLRGAM
                     FLIGLGGGVINGASNALVSDIYEEERGARLSLLGVFFGISAFSIPFLLGLLMKSLGYA
                     RLATLVGAAVAIPILFFLFIRFPEPKQKQGFPLKEGAKLIREAPLLLFGLMLFFESGM
                     EMTMGGWSATFFNEALATESSRAVIYLSFFWLALMAARLLLPALLKRSQAGRLLVSFV
                     AIAFLGTLLLLSAGEAGEALPGLMMVGFGFAAVYPIVLGFVGDRYPQLSGTAFSMVFV
                     MALTGGSLMPWISGLLGKAYGLRMALAIVPAGLILMTLVFTLVRNKYPENKKE"
     gene            complement(4090..5073)
                     /locus_tag="PKM23_04215"
     CDS             complement(4090..5073)
                     /locus_tag="PKM23_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNY90708.1"
                     /translation="MLHLVIRCHQSGGTNTQVLGIDLGGTKIAAALFAQEGKLIRRGS
                     ALLEGRQGQEVGALITATARRLAEGEEIGAIGICIPGIYYARTGRVWAPNIPGWNDYP
                     LLEELQQAFPAITVRVDSDRACYILGETWQGAARGCRNAIFLAVGTGIGAGILVDGRI
                     LRGHDDIAGAVGWMGLTQPWHPKYAACGCFEYHAAGDGLARVAREYLAANPAAPSILR
                     TRPEQELKGADIFTAFDQDDPLAQRILDEAIVHWGMAVANLVSAFNPEKFILGGGVFG
                     PAARFLDRIRAEAQKWSQPIAFSRVTLEISALGGDAGLIGAGSLALAAARE"
     gene            5304..8525
                     /locus_tag="PKM23_04220"
     CDS             5304..8525
                     /locus_tag="PKM23_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY90709.1"
                     /translation="MFARLNTAFFLTLTGLLIIACALPAQAASGKIAGRIVDKASGDP
                     LPGANIIVEAQWEGGKAVSLSGRLGATADLEGYYVILNISPGNYDVRATMVGYIPMVQ
                     RQVRVNLDRTTPLNFGLGQTAVDVGAVQVVAQREVIKADVSSTQEIITTQRIDEAPVM
                     RVDEFVNKIKGVELVANTDGNGLSIRGGAIRETDVRIDGISLRDPRSENSYLSLNSTS
                     VEELQVLTGGFESKYGGFRSGLVNVVTKEGNRDKYTLSVKADMTPATERKFFGNNPWS
                     DDSWIYRVFADTGATGFAWKGTVDNKLVPPELRFFRGWKNKYEGRNNYEAIGLPKSAV
                     LTAEQKRELWLLQHPQYEFAREPDYYLEGTLTGPLPGSWIPLWGALAKRSTIMLGGKY
                     ENTQYAFPIGPRDNYVDWNAQAKITTMMTPAMKFSLNGMYANVNTITAGSASTFGGAL
                     IDNSSRFNFLSSNSASVTQQAALLGGANGFAQMFNKSRLQFYDQRYILSGAKLTHTIS
                     PRTFYDLEFQFSYNDHTLKPFALDTSRADAWISLTDKWRVLNAPTMGTRNGSTNWLTD
                     INNLFWLYGGLQAADSSYTWLASLRGDVTTQIGRHHQVETGFDIRYNYLKVNSGTWAQ
                     SEQSWTPDTWQYFTAKPVEIGLYLQDKLEFQGMIANVGLRAEYFNPQKEPFEVRQPLD
                     EDYANLYNLIYEYLPGRFGSWERWVSFREELDSPTGWPEGGKSSQFKLSPRLGVSFPV
                     TTGSKLYFNYGHFYQRPNIHFIYNLATGPGWASVPSVDLDMARTIAYEFGYEQRFLGS
                     FLFNVTLYYKDIKGEPLSRTYIDYWEEVNVSRYFPDSYKDIRGLEVRLEKNLGQYLTF
                     WGNYEYMLQSRGSSGLATVYENRLKAINEQRWPNLTITEPRPRGNFNLTLHTPRSSGI
                     HNTLKRITPGEGGILDAITWLPGALLGDIFVNLFYEWRDGGRVIVNPQEVERLWKRID
                     VVDYSNLDLRASKMFRVGGVSLEGVLTIQNLLNQKRLSYWNMSTSQYDAYKESLHFPF
                     ETGDQHGDDQMGEWDKEHINIGWFTAPLFLNPRRALVGLRINF"
     gene            8648..10717
                     /locus_tag="PKM23_04225"
     CDS             8648..10717
                     /locus_tag="PKM23_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90710.1"
                     /translation="MSLLLLMHGVGLAQRNYNGPPMFIDVNAINLEMKKLNGEGQFTS
                     QYSWMLTGSDAQNTELFYWPQDQWHSQMLYQIFHPVCLDDSGTVDQLGQHKIIPTPFT
                     SVGRNDYSWEVRRHRPPFIVVDGTPLTREYRWETDPNLSSDIKAVWEDILPFWGIRTR
                     GEIYAFSNPNHSDYLIYKLTFKFTGETKRPIENPIDIDFFPDQKIRLWWPLSFSFGPT
                     KAGEYEVQRGFSFEGEDDLDSWFAYPSRLAGGGVRDSLKIAYYWDATKSGATAYPNGS
                     TDHTGDPDRTNGRLVAPQIPGFALLHAPKSVTDQSEDLSQPFSMPHAGIVADLWGRRD
                     FGLRDTYLGLDSRGKFPLDNISAGFSTSPEKGPMRFITVGPYELTRNAAAARYDSITV
                     VYAIGVGSISDANADSIGRGWFKGDISDQQKRDYMLTGRDSLARTLDRALWAWNRNLN
                     VPDPPPAPDLTVTSDADQNVVEWSYPDPSWFKDPDTGVDDWQAWRVYRKKGAAYVLDP
                     QDRYTGEDWQMIAEITERSTQRYVDTEVTRGVSYYYAVTALDNGTQNTDGLFPGQRLE
                     SSRYANRTGLPAIPFKAGLATTDKVLVVPNPATLNAGSLGFPGAESRILFVNLPYKCK
                     LSVFTETGDRITSFDHFGTDQVVWDQRTDSNQYIVSGIYLLTVTEARDVKGNALPDQY
                     VKFIVVR"
     gene            10775..11800
                     /locus_tag="PKM23_04230"
     CDS             10775..11800
                     /locus_tag="PKM23_04230"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY90711.1"
                     /translation="MKTPMHLFLICALVLMLLSPCGHAEIIKKGQVGFRFLENPVSAE
                     AIGRGGLGLVTLRNSNTLFWNPAGLGWIEEKYDFNINYTRGIADINHSALTGAIRLGS
                     FGVIGLDALIMDYGDFYGTRRAANELGFEETGAFSPQSFAIGLAFSQRVSDRFSYGVH
                     VKYAHQDLGSAWVGVTGTDVDDPNMTMEEKSYALSEPAVDIGAIYDFRLYNIRFGAAM
                     QNFSREIKYEEEKFPLPYAVSFSLSFDPLSIWLGEQDEHQMTAGFETRHPRDFKEKAK
                     YGIEYAYRQQFLLRAGYMGNYDQRGLTLGFGVRQSLGDSKLRIDYAFQDFGLFKSVHS
                     FSFGVSY"
     gene            12292..>14355
                     /locus_tag="PKM23_04235"
     CDS             12292..>14355
                     /locus_tag="PKM23_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNY90712.1"
                     /translation="MTSTMKVPGWVVNGVSVFGHGSIWADISGDSLPDLYIASAVRFA
                     NRKVPETLYISHAGAPYTEEDGARGVTDSYGLTGSHGVIFADYDNDGDLDIFNATTDD
                     RVRLYRNRGDGTFENHTTAATLLSTRVTYDTYGEMGYGTRALVAFDAENDGDIDLYGT
                     NWGPVEDVNGNPWTVPPQPNELFINNGNGTYTNAGDRGCTPVNPSNEGTQGVNAIDID
                     EDGDVDIFVCHRNYTYLGVDAFGQPLWGRGPTPAPNDLFINDGNGNFTEANIRERGMF
                     SESNDINGTTFADYDNDGDLDAFVVYVDEARPNIRVLQNDGKGYFKNVSTDVVIEQWG
                     FTSFIFDMDNDGDLDIFAPRTRDKGRIYLNDGTGKYALQGNSGVEIAMYDPRGGSIAD
                     MDNDGDLDIYYVDANKDTNAAYSNRLFRNDTPTSNRWLKIWGRGPGGDLGAIGTKIWL
                     FEKGHMDEMEHLVGYKQIITGYGYLAQDDIVQHFGVAMRDSVDVRIRMLDGTTLRSRL
                     GTNRRFYFSKPASIAVLDGDNQSGMAGLPLNQPLVVEVRDAQGKAVAGAPVTFTAQGG
                     EGSFAPAGTVHTDGRGIARVVFTAGNTAGGRAITASTGAESALFSLTVINTGPAALTL
                     LSGSGQSAYTGQILADSIRVRVTSSLGAPQSGHPVQFLISAGDGRLKPGDGAAVERLT
                     NSDGIA"
BASE COUNT         2871 a         4535 c         4219 g         2730 t
ORIGIN      
        1 acctgctgcg ctgggaaggt cgcgacgatg cgggcgtggc gctgccgccg ggcgagtatc
       61 tcatccaggc cagagtcgag ggcagagtcg aggtgagacg ggtcacgctg gtgcgctgat
      121 cgatgccggg ccgccgccgg ccgcagcaag gtgcgcggcg gctcagctca accctttggt
      181 cgaccccatc agctcgccga cgttgccctt gtcaaaaaag ccggtgaatg agccgaaacg
      241 ctgaacatat tcggtgatct cgtgactgat cggtgacggg gtgccgaaaa tctgcaggat
      301 gccgcgggtt tcgtcgccga tgagttcttc agtggtgaag gtgagcccga gttcctgctg
      361 gcgctgcaaa acagtgcgga gatcatccgt ataataagcg gtatgatgga cgcgggggcc
      421 atatttggcc gtataggcgt ggactaccga ctgctcgtcc cagccgtaag agacgacgat
      481 ggcgggtttg cggctgcccc agcggaagac catggtctcg gcattttgat cgcccacagt
      541 atagcaggaa tcgaaggagt aaccgctcag tttcataatc atctccgccg tccggtggac
      601 gtgggagagg ggaacgcggt aggcgatgtg atcgagctgg ccgatgttgg cgcggacgtc
      661 cgtgaatggg gctgtgccgg ccgattcagg ggcgatctgc tggggttcca tgccgggcgc
      721 ctcgtgccag gctggtgtgc cttccgggag atagaggaaa cgatctccgg ggccggggat
      781 aggatcggtg gcgatgcccc agggctcctc acggctgggg atgcaggcat tgcgcaggct
      841 gtcgcgcacc ggctggaggt cgcgcacgcg gaaggctagt ccatcgagcc ggggatagcc
      901 ctccaggctc cgtccgttct cgtaaacgat catcgccggc agggagggat cgagcagcag
      961 ctcgtaatag acctcaccag ccttgacctg ccgccaggcc ctggccagac ggtagccggt
     1021 cttgcggccc acctcacgga tcagctcgat cccggtgccc gcagtgacat gatagcggat
     1081 gaaagccaat ccctcgaaga ccggagtgat gggggagggg cccagccgtt tttttagatc
     1141 ctggagttca ccttcgatgc ttttgtgcat agtcctgttc tcaaaccctt gcatgcgact
     1201 gcggagcgcc ctccggagcg gcggcgaagg gcgggggatt ggtaaacaca aagggcggct
     1261 gtcagccgcc cttcgtgttt cggagcttat ttggctgctt ttttagcttt cttctcagcc
     1321 cgtttctcct tgatgctttt ctcggatttc tttttattct ctttgggttt tacatcctga
     1381 tttttagcca tggttttctc cttttttttc gatggacacc aaggtataaa actgaaacca
     1441 aaattaaagc gaatttattc tcggcagtcc ggatccggca gggccggcgg gctttttttg
     1501 atcgtcccgc agagccaaat gacattttac ttgtttttag ctgggcgatt agctaatttt
     1561 tgtggcaaat cttatctatg attccaatca gggagaaaag gaccatgaaa ggcaagaagt
     1621 atttcggctg cgggatgctc ttttttctta ttgccctggg cctctttgct tggtggcgct
     1681 attacttcgt tttcggcgag ggtgtcaagg cgggcgatct caactttctg gtgaaaaagg
     1741 gctatgtctt caagacctgg gaaggccgcc tgatccagac cggcttcaag accccggccc
     1801 ccggctcgat gcaaagcaat gaattcgaat tcagtgtggt ttcggatgag gtcgccaaaa
     1861 agctcgaaaa cgccagcggc aagttcgtcg agctgcgcta caaggagtat ctccatgccc
     1921 tgccttggcg ggggatgagc agatatgtcg tcacccaggt cctccgcatc gaagaacagc
     1981 gggaggaaaa cgatctcccc tattaggcgg cagtaccacg ccgtcgagcc ggattcgccc
     2041 ggtgcgcctt cagcgcaccg ggctttttta ttataccccc gcgatccgcc gcttgtattc
     2101 ggcgagggcc tcatccaggc gctggcgggc ggtggtgtcg cccggcacat tgtggctggg
     2161 atggatgtgc agcttgacgc cgcggtcggc gaggatttcg gccacggtgc agatggtcat
     2221 ccataccgcc gtgacgaagg ccatggtcga gaccgggccg atcttgtcga cgtggttttt
     2281 caggggcacc gaggcgtcct gggccggcac gcaggtgtcg accacgacat cggccagttc
     2341 gaagatctgt ttgcccgagg agtggcgggt ttttactccc gagaactcgc cggctgatcc
     2401 gaaaaccacg atcttcatgc ccaggcgccg ggcctcgagg gcgatgtcga tattgacgtt
     2461 gttgatgccg gaatgggaaa agagccacat gacatcgttc tgatcaaaat tgtaggattt
     2521 catgatctgc tggccgtagc cctcgacccg ttccaggaag acgaactggt ggacgcccat
     2581 ctcgccgacg atgtgggtga agaaggagag gggtatctcg atcatgggat gaaagccgac
     2641 aaagccgccg atgcgggggt acatctcctc gatcggcagg gtcgcatgtc cgcagccgaa
     2701 ggtatggacc caatgcccgc tgccgatggc atcggccatc agcgccgcgg ccttgttgat
     2761 attctcgacc tgggtggatt caatccgatc catcaccccg cgggcgttct ccagccactc
     2821 ttttgcaagc atattctact cctttttatt ttcgggatat ttgttgcgca cgagtgtaaa
     2881 gaccagggtc atcaggatca aaccggccgg cacgatggcg agggccatgc gcaggccata
     2941 ggccttaccc agcagccctg aaatccacgg catgaggctg ccgccggtca gggccatgac
     3001 gaaaaccatg ctgaaggccg taccgctgag ctggggatag cggtcgccta caaagccgag
     3061 gacgatggga tagaccgctg caaagccaaa gcccaccatc atcaatccgg gcagggcttc
     3121 gcccgcttcc cctgcggaga gcagcagcag cgtgcccaga aaggcgatgg cgacaaagct
     3181 gaccagcagc cggccggcct ggctgcgctt cagcagcgcg ggaaggagca gccgcgccgc
     3241 catcagggcc aaccagaaaa atgagaggta gatcaccgcc cgcgagcttt cggtggccag
     3301 ggcctcattg aaaaaggtgg ccgaccagcc ccccatcgtc atctccatgc cgctctcgaa
     3361 gaagagcatc agcccgaaga ggagcagggg cgcctctctg attagcttgg cgccctcctt
     3421 gagcggaaag ccctgttttt gcttgggttc cgggaagcga atgaagagga aaaagaggat
     3481 cgggatggcc acggcggcgc cgaccagggt agccaggcgg gcatagccca ggcttttcat
     3541 gagcaggccg aggagaaagg ggatgctgaa ggcgctgatg ccgaaaaaga ccccgagcag
     3601 gctgaggcgg gcgccgcgct cctcctcgta gatatcggag accagggcat tggaggcgcc
     3661 gttgatgacc ccgccgccga ggccgatgag gaacatcgcc ccgcgcagca gatcgagccc
     3721 ggtcgcccgg ccgatcatga gcagaccggc gaggacaaag gtcgagcaga cgatgagcag
     3781 gatcttgtag ccgtagcggt cgacgatcgg gccgaagagg agagaaccca ggagcatgcc
     3841 gatggtcatg agggggaaga gcgacccggc catggccttg tcgaggccga actgttcgat
     3901 gaccgagggg aggagggcgc cgaggatgga catgacgatg ccgaagatga acatcgccac
     3961 gcaggcggcg gaaaagacgg ctttacggtt atacgacatg gggtgtcctg tggttaggag
     4021 gtgcattgct gcccgccggc gggtgaatag tcctggccgg ccaggtgccg gattcgctgg
     4081 aacgggggat cattcgcgcg ccgctgccag cgccaggctg ccggcgccga taaggccggc
     4141 atcgccgccg agggccgaga tctccagggt caccctggag aaggcgatgg gctgggacca
     4201 tttttgcgcc tctgcccgga tgcggtcgag aaaacgggcg gcgggaccga agacgccgcc
     4261 gccgaggatg aacttttcgg ggttgaaggc gctgaccagg ttggccacgg ccatgcccca
     4321 gtggacgata gcttcgtcga ggatgcgctg tgcgagggga tcgtcctggt cgaaggcggt
     4381 aaagatatca gcccccttga gttcctgctc aggtctggtg cgcaggatgc tgggggctgc
     4441 ggggttcgcg gcgagatact cccgggcgac ccgggcgagg ccgtcgccgg cggcatggta
     4501 ttcgaagcag ccgcaggcgg cgtatttggg gtgccagggc tgggtcagcc ccatccagcc
     4561 gacggccccg gcgatatcgt catgaccgcg caaaatgcgg ccatcgacga gaatcccggc
     4621 gccgatgccg gtgccgacgg cgagaaagat ggcgttccgg cagccccgcg ccgctccctg
     4681 ccaggtctcg ccgaggatat agcaggcgcg atcgctgtcg acgcggaccg tgatggctgg
     4741 aaaggcctgc tgaagctcct cgagcagggg atagtcgttc cagccgggaa tgttgggcgc
     4801 ccataccctg cccgtgcggg cataatagat gcccgggatg cagatgccga tggcgccgat
     4861 ctcctcgccc tccgccagcc ggcgggcggt cgcggtgatg agcgcgccga cctcttgccc
     4921 ctgccggccc tccagcaggg cgctgccccg gcgtattagt ttgccctcct gtgcaaagag
     4981 cgctgcggcg attttggtgc cgcccagatc gatgccaagt acctgcgtat tcgttcctcc
     5041 cgactggtga cagcggatga caagatggag caaaattgcc aaaatataaa cacaaatgca
     5101 agtgaaaaaa acttgcttca cgggaacaca attgctatat tcaataaaga aaacatctta
     5161 ttcctcgaac gagggaagtc tgtttcctga tgcctgaatc aactccccaa caccgtccga
     5221 ctcccgccgc ctcagttggg cgccggcact ccccgaatat cacagcacga cccgcttttt
     5281 catcctcaca aggaggttcc gtcatgttcg cgaggcttaa cacggccttt tttctgacgc
     5341 tgaccggact tttgatcatc gcctgcgcgc tgccggcgca ggcagccagc ggcaagatcg
     5401 ccggccggat cgtcgacaag gccagcggtg accccctgcc cggcgccaac atcatcgtcg
     5461 aggcgcagtg ggagggaggc aaggcggtct ccctatccgg ccgcctgggg gcgaccgccg
     5521 atctggaggg ctattacgtc atcctcaaca tttcgcccgg aaattacgat gtgcgcgcga
     5581 ccatggtggg ctatatcccg atggtgcagc gccaggtgcg ggtcaatctc gaccgcacca
     5641 cccccctcaa tttcggcctc ggccagaccg cggtcgatgt cggggccgtg caggtggtcg
     5701 cccagcgcga ggtgatcaag gccgatgtct ccagcaccca ggagatcatc accacccagc
     5761 gcatcgatga agcgccggtg atgcgcgtcg acgagtttgt caacaagatc aagggcgtcg
     5821 agctggtggc caacaccgac ggcaacggcc tgagcatccg cggcggcgcc atccgcgaga
     5881 ccgatgtccg catcgacggc atctccctgc gtgatccccg ctcggagaat tcctatctct
     5941 cgctcaactc cacatcggtc gaggagctgc aggtgctcac cggcggcttc gagtccaagt
     6001 acggaggctt ccgctccggg ctggtcaatg tggtcaccaa ggagggcaac cgcgacaagt
     6061 ataccctctc ggtcaaggcg gatatgaccc cggccacgga gcgcaagttc ttcggcaaca
     6121 atccctggag cgatgattcc tggatctacc gcgttttcgc cgacaccggc gcgaccggat
     6181 tcgcctggaa ggggacggtc gacaacaagc tggtgccccc tgaactgcgc tttttccggg
     6241 gctggaagaa caagtatgag ggccgcaaca actatgaggc gatcggcctg cccaagagcg
     6301 cggtcctgac cgcggagcag aagcgcgagt tgtggctgct gcagcacccc cagtacgagt
     6361 tcgccaggga gcccgactac taccttgaag gaaccctgac cgggccgctg ccgggctcct
     6421 ggatccccct ctggggcgcg ctggccaaac gatcgaccat catgctcggc ggcaagtacg
     6481 aaaataccca gtacgctttc cctatcggcc cgcgcgacaa ttacgtcgac tggaacgccc
     6541 aggccaaaat caccaccatg atgactccgg cgatgaaatt ctcgctcaac ggcatgtatg
     6601 ccaacgtcaa caccatcacc gcgggctcgg cctcgacctt cggcggggcg ctgatcgaca
     6661 actcgtcccg tttcaatttt ctcagctcca actccgcttc ggtgacccag caggcggctc
     6721 tgctcggggg cgccaacggc tttgcgcaga tgttcaacaa gagccgcctg cagttctacg
     6781 accagcgcta catcctcagc ggggccaagc tgacccatac catctcgccg cggaccttct
     6841 acgatctgga attccagttc agctacaatg accataccct caaacccttc gccctggata
     6901 ccagccgggc cgatgcctgg atatccctga cggacaaatg gcgggtactc aacgccccga
     6961 ccatgggtac acgcaacggc tccaccaact ggctgaccga catcaacaac ctcttttggc
     7021 tctacggcgg gctgcaggcg gccgactcct cctacacctg gctggccagc ctgcgcggcg
     7081 acgtcaccac ccagatcggc cgccatcacc aggtcgagac cggtttcgat atccgctaca
     7141 attatctcaa ggtcaattcc ggcacctggg cccagtccga acagagctgg actcccgaca
     7201 cctggcagta cttcaccgcc aaaccggttg agatcggcct ttacctccag gacaagctcg
     7261 aattccaggg gatgatcgcc aacgtcggtc tgcgcgccga atatttcaac ccccagaaag
     7321 agccctttga ggtgcggcag cccctggacg aggactatgc caacctctac aatctcatct
     7381 atgagtacct gcccggacgc ttcgggagct gggaacgctg ggtctctttc cgcgaggagc
     7441 tcgattcgcc caccggctgg cctgagggcg gcaaaagcag ccagttcaag ctctcgccgc
     7501 gcctcggcgt ctccttccct gtcactaccg gcagcaaact ctacttcaat tacggccact
     7561 tttaccagcg gccgaacatt cacttcattt acaacctcgc caccggtccg ggctgggcga
     7621 gtgtgccctc ggtcgacctc gacatggccc ggaccatagc ctatgaattc ggctacgagc
     7681 agcgcttcct cggcagcttt ctcttcaacg tcaccctcta ctacaaggat atcaaggggg
     7741 agcctctcag ccgcacctac atcgactatt gggaggaggt caacgtctcc cgctacttcc
     7801 ccgattccta caaggatatc cgcggcctcg aggtgcgcct ggagaaaaac ctcggccagt
     7861 acctcacctt ctggggcaac tatgagtaca tgctccagtc gcggggcagc agcggcctgg
     7921 ccacggtcta tgaaaaccgc ctcaaggcga tcaacgagca gcgctggccc aacctcacca
     7981 tcaccgaacc gaggccgcgc ggcaatttca acctgaccct gcacaccccg cgttcctcag
     8041 ggatccacaa caccctcaag aggatcacac ccggcgaggg cgggattctc gatgccatca
     8101 cctggctgcc gggcgccctg ctgggcgaca tctttgtcaa tctcttttac gaatggcgcg
     8161 acggcggccg tgtgatcgtc aacccacagg aggtggagcg gttgtggaaa cgcatcgacg
     8221 tggtggacta ttccaacctc gacctgcgcg cctccaagat gttccgcgtc ggcggggtaa
     8281 gcctcgaggg ggtgctgacc atccagaacc tcctcaacca gaaacgcctc tcctactgga
     8341 acatgagcac ctcccagtat gacgcctaca aggagtccct gcacttcccc ttcgagaccg
     8401 gagatcagca cggggatgac cagatgggcg agtgggacaa ggagcatatc aatatcggct
     8461 ggttcaccgc gcccctcttt cttaatcccc gccgggcgct ggtgggtttg cgcatcaact
     8521 tttaacgaaa gaccgcggga ccggcgccga gccctccggc cgcgccagct cgcttaaaat
     8581 acaagaagac cattctggca aggagcaaaa gatgagccgc agatgcagat acctccccgt
     8641 ttgggggatg agtttactgc tgctgatgca tggcgtcggc ctggcgcagc gcaactacaa
     8701 cggacccccg atgttcatcg acgtcaacgc gatcaacctg gagatgaaaa agctcaacgg
     8761 cgaggggcag ttcaccagcc agtacagctg gatgcttacc ggttcggatg cccagaacac
     8821 cgagctgttt tactggccgc aggaccagtg gcacagccag atgctttatc agatctttca
     8881 ccccgtctgc ctcgatgaca gcggcacggt ggatcagctc gggcagcaca agatcattcc
     8941 cactcccttc accagcgtcg gccgcaacga ctattcctgg gaggttcgcc ggcaccgccc
     9001 gcccttcatc gtcgtcgatg gcacgccgct gacgcgcgaa taccgctggg agaccgatcc
     9061 caacctcagc tcggacatca aggcggtctg ggaggatatt ttgcccttct ggggcatccg
     9121 cacccgcggc gagatctacg ccttcagcaa tcccaatcac agtgattatc tcatctacaa
     9181 gctcaccttc aagttcaccg gcgagaccaa gcgcccgatc gagaatccca tcgatatcga
     9241 tttcttcccc gaccagaaga tcaggctgtg gtggccgctc tccttcagct tcggccccac
     9301 caaggccgga gagtatgaag tgcagcgcgg tttctccttt gagggcgagg atgacctcga
     9361 cagctggttc gcctatcctt cccggctggc cggcgggggg gtgcgcgaca gcctcaagat
     9421 cgcctattac tgggatgcta ccaaatcggg tgcgaccgcc tatcccaacg gcagcaccga
     9481 ccacaccggc gatcccgacc gcaccaacgg ccgcctggtc gcgccccaga tccccggctt
     9541 tgccctgctg catgccccca aatcggtcac agaccagagc gaggatctct cgcagccctt
     9601 cagcatgccg cacgccggca ttgtcgccga cctctggggg cgacgcgact tcggcctgcg
     9661 cgacacctat ctcggcctcg acagccgggg caagttcccc ctcgacaata tctcggccgg
     9721 tttctcgacc tcgccggaaa aaggcccgat gcgcttcatc accgtcggtc cctacgagct
     9781 gaccaggaac gccgccgccg cccgctacga cagcatcacc gtggtctatg ccatcggcgt
     9841 gggctcgatt tccgatgcca acgccgactc catcggccgc ggctggttca agggcgatat
     9901 ctccgaccag cagaaacggg actatatgct caccgggcgc gattctctgg ccaggaccct
     9961 cgaccgcgcc ctctgggcct ggaaccgcaa cctcaacgtc cccgatcccc cgcccgctcc
    10021 ggacctgacc gtcaccagcg acgccgacca gaatgtcgtc gaatggtcct atcccgaccc
    10081 ctcctggttc aaggaccccg acaccggcgt cgatgactgg caggcctggc gcgtctatcg
    10141 caaaaaaggc gccgcctacg tcctcgatcc ccaggaccgc tacaccggcg aggactggca
    10201 gatgatcgcc gagatcaccg agcgctccac ccagcgctac gtcgacaccg aggtgacccg
    10261 cggcgtcagc tattattacg ctgtcaccgc cctcgacaac ggcacccaga acaccgacgg
    10321 cctcttcccc ggtcagcggc tggagagctc gcgctatgcc aaccgcaccg gactgccggc
    10381 catccctttc aaggcggggc tcgccaccac cgacaaggtg ctcgtcgtcc ccaatccggc
    10441 gaccctcaac gccggcagcc tcggctttcc cggcgccgaa tcgcgcattc tcttcgtcaa
    10501 tctgccctac aagtgcaagc tcagcgtctt caccgaaacc ggcgaccgga tcacctcctt
    10561 cgaccatttc ggcaccgacc aggtggtgtg ggaccagcgc accgattcca atcagtatat
    10621 cgtcagcggc atctacctcc tcaccgtcac cgaggcccgg gatgtgaagg gtaacgccct
    10681 gcccgatcag tatgtcaaat tcatcgtggt gcgctgaatg gaacccgaaa cccctggagc
    10741 ctgcgctctg agctacttaa ccgagggaga tgttatgaaa acccctatgc acctctttct
    10801 aatatgcgcc ctggtcctga tgctcctgag cccctgcggg catgccgaga tcatcaagaa
    10861 agggcaggtg ggcttccgct tcctggagaa cccggtctcc gccgaagcga tcggccgcgg
    10921 cggcctcggg ctggtgaccc tgcgcaacag caacaccctc ttctggaatc ccgccggcct
    10981 gggctggatt gaagaaaaat atgattttaa catcaattac acccgcggca tcgccgacat
    11041 caaccacagc gccctgaccg gggccatccg gctcggcagc tttggggtga tcggcctcga
    11101 cgccctgatc atggattatg gcgatttcta cggcacccgc cgcgccgcca atgaactcgg
    11161 cttcgaagag accggggcct tttcgcccca gtccttcgcc atcggcctcg ccttcagcca
    11221 gcgcgtgagc gaccgcttct cctacggcgt ccacgtcaaa tatgcccacc aggacctcgg
    11281 ttccgcctgg gtcggggtca ccggcaccga tgtggacgat ccgaacatga ccatggagga
    11341 gaaaagctac gccctctccg aacctgctgt cgacatcggc gccatttatg actttcgcct
    11401 ctacaacatc cgcttcggcg cggccatgca gaacttttcc cgggagatca agtacgagga
    11461 ggaaaaattt ccgcttccct atgcggtcag tttcagcctc tccttcgatc ccctctcgat
    11521 ctggctgggc gagcaggatg agcaccagat gacggccggc tttgagaccc gccacccccg
    11581 tgatttcaag gaaaaggcca agtacggcat cgaatatgcc tacaggcagc agtttctcct
    11641 ccgcgccggt tacatgggca actatgatca gcgcggcctg accctcggct tcggggtgcg
    11701 ccagtccctt ggcgactcga aactgcgcat cgattacgcc tttcaggatt tcggcctctt
    11761 caaatcggtg cactccttct ctttcggcgt cagctactag ggacgcctgc gagtcgtccc
    11821 gccgcccgcg gacgccccgg ctctctgctc tcacctcctt caatgccttc aggggtggat
    11881 tttcccgccc cggtccagca cggaccgggg ctcttttttg ctctcccgga aagctatcct
    11941 tctgttgtaa taatatataa ataaatgagt tgaatggacc gccatttccg gcttcaaacg
    12001 cacgccatcc agcccagggg cgaaaaatcg actctccgca cgaattgggc ctgattgttg
    12061 cttaaaagtg ggcgatgagc gggggccaat tctaacgcag ctttggtcag gcattgctat
    12121 atccatgtaa ttcaataaat tgtatgcttc ggagcattcc ttatgcctct gcgcttgaag
    12181 agacgaattg actcactgta tcacacctgt ttaaaaaaga gactgctggt cccgctcctg
    12241 ctgctgtttc tgcccgggct cggagctgcg cagaccatca ggttcacgga tgtgacctcg
    12301 acgatgaagg tccccggctg ggtggtcaac ggggtcagcg tcttcggcca tggcagcatt
    12361 tgggccgaca tctcgggcga ttccctgccc gatctctata tcgccagcgc cgtccgtttc
    12421 gccaaccgca aggttcccga gaccctctat atcagccatg ccggcgcccc ctacaccgag
    12481 gaggacggcg cccgcggcgt gaccgacagc tatggactga ccggatccca cggtgtgatc
    12541 tttgccgatt atgacaatga cggcgatctg gatatcttta atgccaccac tgatgaccgc
    12601 gttcgtctct accgcaaccg tggggacggc acctttgaaa accataccac cgccgccacc
    12661 ctgctctcga cacgcgtcac ctacgacacc tatggcgaga tgggctatgg tacccgcgcc
    12721 ctggttgcct tcgatgccga aaatgacggg gacatcgacc tctatggtac caactggggg
    12781 ccggtcgagg atgttaacgg caatccctgg acggtgccgc cccagcccaa cgaacttttt
    12841 atcaacaacg gcaacggcac ctacaccaac gccggtgatc gcggctgcac tccggtcaat
    12901 cccagcaatg agggaaccca gggggtgaac gcgatcgaca tcgatgaaga cggtgatgtc
    12961 gacattttcg tttgccatcg caattatact tacctagggg tcgacgcctt cggccaaccc
    13021 ctctggggac gcgggccaac cccggccccc aatgacctct tcatcaacga cggcaatggc
    13081 aatttcaccg aggcgaacat ccgcgagcgc ggcatgttct ccgagagcaa cgacatcaac
    13141 ggtaccacct tcgccgatta tgacaacgat ggcgacctcg atgccttcgt cgtctatgtc
    13201 gatgaggccc ggcccaatat ccgcgtcctg cagaacgacg gcaagggcta tttcaagaat
    13261 gtcagtacgg atgtggtcat cgaacagtgg ggcttcacat ctttcatttt tgacatggat
    13321 aatgacgggg atctcgacat cttcgccccg cgtacccgcg acaaggggcg gatctacctg
    13381 aacgacggta ccggcaagta cgccctgcag ggcaacagcg gcgtcgagat tgcgatgtat
    13441 gacccccgcg ggggttcgat cgccgatatg gataacgacg gcgatctcga catctactat
    13501 gtcgacgcca acaaggacac caatgcggcc tacagcaacc gcctgtttcg caacgataca
    13561 cccacttcca accgctggct caagatctgg gggcgcggcc ccgggggcga tctcggcgcc
    13621 atcggcacca agatctggct cttcgagaag ggccacatgg atgagatgga acatctggtc
    13681 ggctacaagc agatcatcac cggctatggc tatctggccc aggatgatat cgtccagcat
    13741 tttggtgtcg ccatgcgtga ttccgtcgat gtcaggatcc ggatgctgga cgggaccaca
    13801 ctcaggagcc gtctcgggac caaccgcagg ttttatttca gcaagccggc ctcgatcgcg
    13861 gtgctcgacg gcgacaatca gagcgggatg gcggggctgc ccctgaatca gcccctggtc
    13921 gtggaggtca gagatgccca gggcaaggcg gttgccggtg cgccggtgac ctttacggca
    13981 cagggtggtg agggcagttt cgcccccgcc gggacggtcc atactgacgg gcgcggcatc
    14041 gcccgggttg ttttcaccgc cggcaacacg gcgggggggc gggcgatcac ggcgtcgacc
    14101 ggggctgaga gcgctctttt ttccctcacg gtgatcaata ccggcccggc cgctctgacc
    14161 ctgctctcgg gcagcggcca atccgcctac accggccaga ttctggccga ctccatccgg
    14221 gtgcgcgtga ccagcagtct gggggcgccc cagagcggcc atcccgtgca gttccttatc
    14281 tctgccgggg atggacggct taagcctgga gatggtgccg ccgtggaacg cctcaccaac
    14341 agcgacggca tcgct
//
LOCUS       DAOPWU010000047        14317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4326_length_14317_cov_4.493409, whole genome shotgun sequence.
ACCESSION   DAOPWU010000047 DAOPWU010000000
VERSION     DAOPWU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4326_length_14317_cov_4.493409"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1994
                     /locus_tag="PKM23_04240"
     CDS             <1..1994
                     /locus_tag="PKM23_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90713.1"
                     /translation="KDYYHPVGNENKAVNTYANANFHNFRSGCWIVAKDLMTPGPPPK
                     NAPTPTDYQAFLSGLQEDTYGIELPLKNLPMRTNYIESPDFDPLLPEEMTDITFHTNT
                     GVSVTRRTYVWSFPGYSDFIIYDYLFENTGIMVSDFTSAVVPDFPQQELKDLYFVFHS
                     GISVSTKSNINFGAELSPIHAGGFGWEKKWYHDYYHSFDDGTLVFSTNFNGGKEPPPW
                     WDASALKENQIWKNYFGEELMSPSAFGWLALYASPRDGQPRTSPKPDVLRIDSHKGGR
                     FQGMDLDLEKMTIGNFRPKKTFYDFAQVPDLQDKLGNTGNRFNFFTFSYGPYNLSPGQ
                     QARFILAEIAGCMDYRDVVNGDPQGHFPDSTIMAIRRNAVFARNAVKWGMGEEVNGML
                     LAADIPEPPPGPNCVAANVSYSTEVPLVRVAWDKAAETAVIKDASGGVFYDGSTDLDG
                     YRIYRSSDFQFLNETEPPVFRGAYWDLILDIPRAEFAKYWNESEQQYILMDSTVVFGF
                     RCGYYVAAYNENPRPWTSANGTVVTDLPALQNGDYNRTNPVAASPGPVNTFDIYVVPN
                     PYIFNDPDRSFGINDPYRIEFRSLPESCTIRIYTLSGDLISTIEHEPDRFGNLSGSAV
                     WNQKSDSGLQVAPGLYIYHIESRVPGVNSRLTGKIMIIR"
     gene            2018..3004
                     /locus_tag="PKM23_04245"
     CDS             2018..3004
                     /locus_tag="PKM23_04245"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY90714.1"
                     /translation="MKRSLSFAVLLSLIAASALFGFNKGGRTVFQFSKIGVGARQAGM
                     GEACIAVVRDVNAVYWNPANIGGLAGGQAAFTYTRWFADLNYLTAAVGYRWNGVGVFA
                     LSFAGLDYQDIQEALVTSTSGAADTRTGKNFSGGDLQLGLSYSRDFTDKLSIGISAKY
                     FREELFIYNVGLVAFDVGTYYETGFKGFRIAMSAQNFSTGSVSWLGKEKSDRIEGYEI
                     PLIYRIGTSFDLINNENSFIDLGPDHHLQLAIDAINTNDYGERYHLGGEYWYNHFIAL
                     RCGYRFNYEEGNLAAGFGLHHQLSGMKFQFDYAYVNYEFLESPHRFSLLMSF"
     gene            3035..6301
                     /locus_tag="PKM23_04250"
     CDS             3035..6301
                     /locus_tag="PKM23_04250"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNY90715.1"
                     /translation="MAFAKITLDGEKLTRENFARPPREMGILPFWFWNGDLDEQEMEW
                     QLREYQDKGIHGLFIHGRFGLKVPYLSEGWFERVKFVIQAARRIGIDIWVYDEMNWPS
                     GTAERQVPARYPHLRQKYLELVVLNVDGPLFTFLEATDSRYVNTGDSRPIAAFACSAE
                     EFEKGITHLIDLTPNLSFNKVVPWEAPAGKWKLLYFLEKEIDYYIDTLDPESTRRFLE
                     LTHEGYKKAVGEEFGAIVPGFYTDEPAMYYYQVGVDNYVVPWSSRMFKIFRKRRGYDL
                     KPWLPALYADMGERTAAIRYDFWRTLTEQYADSYYKQIRDWCDENGVIFTGHLLFEEW
                     LRGHARCEGNIFKYLKHMHLIGVDHLYPKIGSEEEPAEHVSLKMASSAAHHFGSTRLL
                     CESMGGTYWDCTLERMKWIANWEYVLGVNLFNNHGYHYSIEGERKRDWPPSQFYHHTW
                     WKYYSRFTTYMARLGHALSGGRHVAKILILYPINSIWTNYTPQERNRIGTLIESDYNY
                     LTDTLLRLHFDFDYVDEDVLIDAGIAEGKIRIAEEAYDLLILPPLTHVKADTFTAIEK
                     FASCGGRIIADTLLPLEFLESRENEARRRVKELFGADPLQLLQQFESGAPLEIKRLGE
                     GGRLALVQGQGLHQHKPKDEFMRLLGEMLTPDVIIGHEAVFYLHRIKDGHDLYFLVNT
                     LQQELGRVEVSFEKSGTPELWDPDSGEIRPLHVHEIRDGRLVLQLDFPPAASHFVIIR
                     DEQPALRIIGASVHIERFDSGRATGYADAQASEPFIRVDEGGKLRELHARALDPLPDL
                     PLGPPLEFSLEGDNVLCISRWKMRVEETGESGLEPARPDFNDEGWLDVTLGAWEMQLP
                     QEREEESYPAALWYRTRFLMDCMPGDLRLLIDGFSGSAHRLFINGEEIKDKGRRSPLD
                     AEIREVDIRPFIRTGRNVVAVRLVAERRTDGILDLLKLVGTFALAGSSDGYRITEPAG
                     RIAVGDWTKQGYPFFSGTGLYTFEVNLPSHYLQGKLLLAAECGEDVLEVSVNGDSAGI
                     LPWHPYRIDLTGRLQPGRNRLTLKVTNTLINILEAVQKPSGLFAEPRLIHMPVYEFTL
                     "
     gene            6334..7251
                     /gene="rbsK"
                     /locus_tag="PKM23_04255"
     CDS             6334..7251
                     /gene="rbsK"
                     /locus_tag="PKM23_04255"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNY90716.1"
                     /translation="MDRNAILVVGSANMDMVVVAEKFPQPGETLFGRQFNMYPGGKGA
                     NQAVCAAKLGGETHFIGRMGNDLFRERLVQNMASDGVVMDHLLIDEQEPTGIALITVD
                     GGGENEIIVISGSNMKLSPADVESRLALFNRVGVVLAQLEIPLETVARTAELAAAAGA
                     CFILNPAPACKLPVKLLQRVDFLTPNRLELELLAGMSILDDIESVRCAAQRLLDQGVG
                     NVLVTLGERGSLWVTPGEARLFAARRVQAVDTTAAGDAFNGAFAFALSRGETVPQAIH
                     FANRVAAFSVTRMGAQSSMPTAADIEEWV"
     gene            7347..7757
                     /gene="rbsD"
                     /locus_tag="PKM23_04260"
     CDS             7347..7757
                     /gene="rbsD"
                     /locus_tag="PKM23_04260"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HNY90717.1"
                     /translation="MKKQGILNAQLSYLVARMGHTDRLVICDSGLPIPPEAEVVDLAL
                     TWNIPAFLDTLRTVLGELHIEGAVVAAEMEQVSNTLYKQTLQLLPGIPVEKVAHEQFK
                     ELTRAPGTIAFVRTGEATSYANIMLVSGVNFNHS"
     gene            7771..9216
                     /locus_tag="PKM23_04265"
     CDS             7771..9216
                     /locus_tag="PKM23_04265"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNY90718.1"
                     /translation="MRKEYIAALLLLAMAAFAACGDTAGSRYDRLVRPADATYYHVPV
                     GLCEDYPEESTTLTGIRKDMEFLKESKVDLLRISFGWDAIEAEKDKYDWLFWDDFVKM
                     AVEEYGITLIPYICYMPRWNSTKPEDNNWFWNYPPVDPQQFGEFIKLLVNRYKKYIKT
                     WELWNEPDIWVYWQGSPEQFAEMIRIGARAVKEADPDARVVLGGVAYSEEFVAKMFRD
                     FGLSPYIDIVNMHNYFETWSERPVEDIDDHILKMTDVIRQYGNRQPLWMAEVGYSTFR
                     QGSKISSEYTANYDYEHTPEYQAVDLFKRLALIVSTEKVSAVAWYEISDLPPSDEVIG
                     DEYNNRHLGVAFVNYVPKPARKALMFFNSLFGDEYRNITGQAAITRPENSDSHALVFE
                     KVDGSVVIVSWLQTALPGKRSADTSGTVRDERRESISVSLPMALQGSARLYDHLGEGK
                     ALENIRREGGMTLIPEIVLEGGAVCIIHIEK"
     gene            9245..9916
                     /locus_tag="PKM23_04270"
     CDS             9245..9916
                     /locus_tag="PKM23_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HNY90719.1"
                     /translation="MKRSEINHLQRAAMEFFGRHCFHLPPWARWGKKEWMENREAAGE
                     IFNNKLGWDLTDFGSGDFYTIGLLLFTIRNGSPGDPQGKGYAEKVMIVREGQVTPWHF
                     HWSKMEDIINRGGGNLVIELANSTPDEKLAGGRVQVQVDGLTRSVEAGGKIVLTPGES
                     ISLPAYLYHKFYGEAGKGTVMVGEVSMVNDDDKDNRFLDPIGRFPVIDEDEEPLYYLC
                     NEYPV"
     gene            9940..10428
                     /locus_tag="PKM23_04275"
     CDS             9940..10428
                     /locus_tag="PKM23_04275"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HNY90720.1"
                     /translation="MIILRPKQVTPANFARFGSVVTAPQTAPTSQAADYKFWSDLAHY
                     RIDGETEIGICTVYHMPVSIITEVERHEHTPEILIPINAPFALPLLRDGDSVEQAELF
                     RVGIGEAVVIDAGIWHGPCLPIGCANSSYFVIFARRTPHADVRKKEISPIEIMQKGQD
                     RG"
     gene            10425..12095
                     /locus_tag="PKM23_04280"
     CDS             10425..12095
                     /locus_tag="PKM23_04280"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HNY90721.1"
                     /translation="MILSNSKTAFIFADQPDQISEIKLADGTPVGSTSRQAILIRTPA
                     AVSEPVLLSRVSSSASGPASAELLFQDESGAWRAELQIHAAEQGLRFRLQAYAPEPIW
                     LVEWRLSGLQFDEVIIPALGGQALSGAMPAETTLSYKYPFWWNAQFVVGKKGGGGLWL
                     FSRDEKPDLKLLRVGRSSDGFAWTYGCEARPPFPCTLEAEWYLDGINGGWEVAVERHQ
                     RWLEEAFDLRPLQQKASFPAWAREINFVLELWGARRDSIAPMHTFAQMVERLQTWRGM
                     HDPRQTLVYLPGFAMHGIDSHAPDYHPSAQCGGAAEFARLIEAAHSWGFRVMIHTNVL
                     AMTFNHPHFPRFREHQVIDCFGRPQGWGLDMDGDWLAEPYFAYINPGVRAWGDLMVDV
                     IGDLVRRFGIDGVFLDQTLLAFNVSRGPNFIEGMRTHIRRLQEAMPEILFAGEGMHEH
                     LLNPLPMAQIHGIDSITEVHGMEGQLSWRSAHPVSTALFSPYCRFMAHLLTRHPSHPM
                     FTFQESAYAKLHVIPALCLYSAAQAMDLPEVKEMIARAQQLTLSDGFR"
     gene            12123..13049
                     /locus_tag="PKM23_04285"
     CDS             12123..13049
                     /locus_tag="PKM23_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNY90722.1"
                     /translation="MKLGFLTACLPDIALADLLQWAQENQFQALELAAWPVASDRDYQ
                     ARQIDAARMTPAEAGRVTELFREHDLEISALAYYDNNLHPDLAQREGYHDHLRRVIDT
                     AALLQVGLVGTFVGARPDLSPAENIREIGGVFRGLTAYAADKGVRIMIENCPMENWVR
                     FGFPGNYAFSPELWSALFNEVPSDNFGLNLDPSHLHWLGIDYLRAVREFAPKIFHAHA
                     KDTEILPEGRHHHSIFGTQIDPAPWKSGWWRYRLPGLGEIDWRAFISALQENGYDYVL
                     SIEHEDPVWEGSEEKIKRGLVLGRKHLSPFII"
     gene            13064..>14317
                     /locus_tag="PKM23_04290"
     CDS             13064..>14317
                     /locus_tag="PKM23_04290"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNY90723.1"
                     /translation="MRQLLKEEYIPFGSQYYRAPLPHAGEWERDLDRMAALGFNTVKF
                     WVQWRWNHPTEDEFRFEDIDRLMELALARKLHVMLNTIFDVAPAWIYRLYPDASMVTL
                     DGRRIGPQTQPHRQIGGLGYCFNHDGVISHFFRFLAATVERYKEHPALAIWNVGSEPE
                     LTSSMAEMRLYADDAGRMGDMLCYCPNCQAKFRLWLAGKYDSIDRLNECWNRNYRSFD
                     EAELPITRNTFNDLIDWRMFFVHTLGENVRRRFEIAREIDQGRHPIMCHHVFVQGFPV
                     TSTANDPWNIGQYGDLHGFTQMDDAMMIDVLRSCARGKPVISAEMLALPGYTLDLPAI
                     IDANTVKRFIFSGVAGNQKGFIFWQYRPEILGREAPTWGLSGLDGEETPWLKAYAEAG
                     RVLQKNATFLLDAVPQRARIALLYHP"
BASE COUNT         2971 a         4331 c         4042 g         2973 t
ORIGIN      
        1 tcaaggacta ttatcacccc gtcggcaatg agaacaaggc ggtcaacacc tatgccaacg
       61 ccaattttca caacttccgc agcggctgct ggatcgtcgc caaggatttg atgaccccgg
      121 ggccgcctcc caagaatgcg ccgacgccca ctgactatca ggcctttctc tccggtctgc
      181 aggaggatac ttacggcatc gagctgcctc tcaagaacct gccgatgcgc accaactata
      241 tcgagtctcc ggatttcgat cccctgcttc ctgaggagat gaccgacatc acctttcaca
      301 ccaataccgg cgtgagcgtg accaggcgca cctacgtctg gagctttcca ggttacagcg
      361 atttcatcat ctacgactat ctctttgaaa atacaggaat catggtctct gatttcacca
      421 gcgcggtggt ccccgatttt ccccagcagg agctcaagga tctctatttt gttttccatt
      481 ccggtatctc ggtttcgacc aagagcaaca tcaatttcgg cgccgaactc tcgcccatcc
      541 acgccggcgg tttcggttgg gagaagaaat ggtaccacga ttattatcac tcctttgacg
      601 acggcaccct ggtcttctca accaacttta acggcggcaa ggaaccgccc ccctggtggg
      661 atgccagcgc gctcaaggag aaccagatct ggaagaacta tttcggtgag gagctgatga
      721 gcccctccgc cttcggctgg ctcgcccttt atgcgagtcc gcgggacggt caaccccgca
      781 cttcgcccaa gccggatgtt ttgcgcatcg attcccacaa gggcgggagg tttcagggca
      841 tggacctcga cctggaaaag atgacgattg gcaatttccg gcccaaaaag accttttatg
      901 atttcgctca ggttcccgac ctgcaggata agctgggcaa caccggcaac cgcttcaact
      961 tttttacctt ttcgtacggg ccttacaatc tgtcccccgg ccagcaggcc cgcttcatcc
     1021 tggccgagat cgccggctgc atggactacc gcgatgtagt caatggtgat ccccaggggc
     1081 atttcccgga cagcactatc atggccatcc gccgcaacgc tgtcttcgcc cgtaatgcgg
     1141 tcaagtgggg aatgggcgag gaggtgaacg gcatgctcct ggccgccgat atccccgagc
     1201 cgccgcccgg ccccaactgc gtcgctgcca atgtctccta cagcaccgag gtgcccctgg
     1261 tgcgcgtggc ctgggacaag gccgccgaaa ccgctgtcat caaggacgcc tcgggcgggg
     1321 tcttttacga cggcagcact gacctggacg gctaccgcat ctaccgctca agcgatttcc
     1381 agttcctcaa cgagaccgaa ccgccggtat tccgcggcgc ctactgggac ctcatcctcg
     1441 acatcccgcg cgcagagttc gccaaatact ggaacgaatc cgagcagcag tatatcctga
     1501 tggattccac agtggtcttc ggcttccgct gcggttatta tgtcgcggcc tataacgaaa
     1561 atccgcgccc ctggacctcc gccaacggca ccgtcgtgac ggacctgccc gcactccaga
     1621 acggagacta taaccgcacc aatccggttg ccgcatcccc gggcccggtc aacaccttcg
     1681 acatctatgt tgttcccaat ccttacatct ttaatgatcc cgaccgcagt ttcggcatca
     1741 acgatcccta ccggatcgaa ttccgcagcc ttcccgagag ctgcaccatc cgcatctata
     1801 ccctctccgg cgacctgatc agcacgatcg agcatgagcc ggaccggttc ggcaacctct
     1861 ccggcagcgc ggtctggaat cagaagagcg attccggcct gcaggtggcc cctggcctct
     1921 acatctatca catcgaatcc cgcgtccccg gtgtcaacag ccggctgacg ggcaaaataa
     1981 tgattattcg ctaagccatt caaggatggg ataagatatg aaaagaagcc tctcatttgc
     2041 ggttctgctc tcgctgattg ccgcatcagc cctcttcggt ttcaacaaag gcgggcggac
     2101 ggtgttccag ttcagcaaga tcggcgtcgg cgcccgtcag gcgggcatgg gcgaggcctg
     2161 tatcgcggtt gtgcgcgatg tcaacgcagt ctactggaat cccgctaaca tcggcggtct
     2221 ggccggcgga caagccgcct tcacctacac gcgctggttt gcggatctga attacctcac
     2281 cgctgctgtt ggctaccgct ggaatggagt gggggtcttt gccctctcct tcgccggtct
     2341 cgactaccag gatatccagg aggccctcgt gaccagcacc tccggcgcag ccgatacacg
     2401 caccggcaag aacttcagcg gcggcgatct ccaactcggc ctctcgtatt cgcgtgattt
     2461 taccgacaaa ctttcgatcg ggatttccgc caagtacttc cgcgaagaac tcttcatcta
     2521 caacgtagga ctggtcgcct ttgatgtcgg cacctattat gaaaccggct tcaagggctt
     2581 tcgcatcgcc atgagcgccc agaatttttc caccggatca gtcagctggc tcggcaagga
     2641 aaaaagcgac cggatcgagg gttatgagat tcccctcatt tatcgcatcg gtaccagctt
     2701 tgatctcatc aacaacgaga acagctttat cgatctcggg cccgaccatc atctccagct
     2761 tgccatcgac gccatcaata ccaatgacta tggcgaacgc tatcacctcg gtggcgagta
     2821 ctggtacaat cacttcatcg ccctccgctg cggctaccgc ttcaactatg aggagggcaa
     2881 tctggccgct ggtttcggct tgcatcacca gctttcgggc atgaagttcc agttcgatta
     2941 cgcctacgtc aactatgaat ttctcgaatc accccaccgc ttttcgctgc tgatgtcgtt
     3001 ttgatccggc ggattaactg aggagagttc ctgaatggcc tttgcgaaga ttacccttga
     3061 tggtgaaaaa ctgacccggg aaaatttcgc ccgtccgccc cgggagatgg gcatcctgcc
     3121 tttctggttc tggaacgggg atctggatga gcaggagatg gaatggcagc tgcgcgaata
     3181 ccaggacaag ggaatccacg gacttttcat acacggccgc tttggattga aagtgcccta
     3241 tctctccgaa ggctggttcg aaagggtgaa atttgtcatc caggccgcgc gcaggatcgg
     3301 catcgatatc tgggtctatg atgagatgaa ttggccaagc ggcactgcag agcgccaggt
     3361 gcccgccaga tatccccacc tcaggcagaa atacctcgag ctggtcgttc tcaacgtcga
     3421 cggcccgctc ttcactttcc ttgaagcgac cgattcgcgc tacgtcaaca ccggcgattc
     3481 acggcccatc gccgccttcg cctgctccgc cgaagaattc gaaaagggca tcacccatct
     3541 catcgatctg acccccaacc tctcctttaa caaggtggtg ccctgggagg cccctgcagg
     3601 caagtggaag ctgctctact ttctcgagaa ggagatcgac tattacatcg acacccttga
     3661 tcctgaatcc acccgtcgtt ttctcgaatt gacccatgag ggctataaaa aggcggtcgg
     3721 cgaggagttc ggcgccatcg ttccgggctt ttacaccgat gagccggcga tgtactacta
     3781 ccaggtgggc gtggataact atgtcgttcc ctggtcgagc aggatgttca aaatcttccg
     3841 caaacggcgc ggctatgacc tgaagccatg gctgcccgcg ctctatgccg acatgggcga
     3901 aagaaccgcc gccatacgct acgatttctg gcgtaccctc actgagcaat acgccgacag
     3961 ctattacaaa cagattcgcg actggtgcga cgagaacggc gtgatcttca caggccatct
     4021 cctcttcgag gagtggctgc gcggacatgc tcgttgtgag ggcaatatct tcaagtatct
     4081 caaacatatg cacctcatcg gagtcgatca tctctaccca aaaatcggca gcgaggagga
     4141 acctgctgag catgtctcgc tcaagatggc cagctcggcc gcccaccatt tcggcagcac
     4201 ccgtctgctc tgtgagtcca tggggggaac ctactgggac tgcaccctgg aacgcatgaa
     4261 atggattgcc aactgggagt acgtcctggg ggtcaacctg ttcaacaacc atggctacca
     4321 ctattccatc gagggcgagc gcaagcgtga ctggccgccg agccagttct atcaccacac
     4381 ctggtggaaa tactacagcc ggttcaccac ctacatggct cgcctgggcc atgccctctc
     4441 tggcggccgg catgtggcca aaatactgat cctctatccg atcaacagca tctggaccaa
     4501 ctataccccg caggagcgca accggatcgg caccctcatc gaatcagact ataactacct
     4561 gacggacacc cttttgcgcc tccatttcga ctttgactat gtcgatgagg atgttctgat
     4621 cgatgccggg atcgcggagg gtaaaatccg tatcgcggag gaggcgtatg acctgctcat
     4681 ccttccgcct ctgacccatg tcaaggcgga taccttcaca gccatcgaaa agttcgccag
     4741 ctgcggcggc aggatcatcg ccgataccct gctgcccctc gaatttctcg agagcaggga
     4801 gaatgaagcc cggcggcggg tgaaagagtt gttcggcgcc gatccgctgc agctgctgca
     4861 gcagttcgag tccggcgccc cgcttgaaat aaagcgcctc ggagagggcg gacgtctggc
     4921 cctggtgcag ggccagggtc tgcaccagca caaacccaag gatgaattta tgcgcctgct
     4981 cggggagatg ctcacccccg atgtgatcat cggtcacgag gcggtttttt acctgcaccg
     5041 catcaaggac ggacacgatc tctattttct ggtcaatacc ctccagcagg agcttggcag
     5101 ggtggaggtg agttttgaaa agagcggcac gccggaactc tgggatccgg acagcggcga
     5161 gatcagaccg ctgcatgtgc atgaaatacg cgacggccgg ctggttttgc agctcgattt
     5221 tccgccggca gcctcgcatt tcgtcatcat cagggatgaa cagcctgctc tgcggataat
     5281 cggcgccagc gtacacatcg agcgttttga cagcggcagg gcaaccggat atgccgatgc
     5341 gcaggcgagc gagcctttta tccgtgtcga tgagggagga aaactccggg aacttcacgc
     5401 cagggcgctt gatcccctgc ccgatctacc gctgggcccg cccctcgaat tcagcctcga
     5461 gggggataac gtgctctgca tctcccgctg gaagatgcgg gtcgaggaga ccggagagtc
     5521 cgggcttgag cccgcccggc ccgatttcaa tgacgagggc tggctcgatg tcaccctcgg
     5581 cgcctgggag atgcagcttc cacaggaacg cgaagaggag agttacccgg cggcgctttg
     5641 gtatcgcacc cggttcctga tggactgtat gcccggcgat ctccgtctgc tcatcgacgg
     5701 tttcagcggc agcgcccatc gcctcttcat caacggggag gagattaagg ataagggcag
     5761 gcgcagtccc ctcgacgcgg agattcgcga ggtggatatc cgccccttca tccgcaccgg
     5821 gcgcaatgtc gttgccgtac gcctggtcgc agaacgacgc accgacggca ttctcgatct
     5881 gctcaagctc gtcggcacct tcgccctggc cggctcctcc gatggctacc gcatcaccga
     5941 gccggccggt cgcatcgccg tgggcgactg gacgaaacag gggtacccct ttttctcggg
     6001 caccggcctc tacacctttg aggtcaatct gccgtcgcat tacctgcagg gcaagctctt
     6061 gcttgcggcc gagtgcggcg aggacgtgct cgaggtttcg gtcaatggag attcggccgg
     6121 catcctcccc tggcatccct atcgaatcga tttgaccggc cggctgcagc cagggcgcaa
     6181 tcgccttacc ctcaaggtga ccaacaccct catcaatatc ctcgaggcgg tgcaaaagcc
     6241 gtcgggactt tttgctgaac cccggttgat ccacatgccg gtgtatgaat tcactctgta
     6301 ggggactata gcaaccagga agacgggaaa aacatggatc gcaatgcaat tctggtcgtc
     6361 ggcagtgcca atatggatat ggtcgtggtg gcggagaaat ttcctcagcc tggtgaaacc
     6421 ctttttggcc ggcagttcaa catgtatcca ggcggcaagg gggccaatca ggctgtctgc
     6481 gccgccaagc tgggagggga gacccatttc atcggcagga tgggcaacga tcttttccgg
     6541 gagcggctgg tgcagaacat ggccagtgac ggagtggtga tggatcatct tctcatcgat
     6601 gagcaagagc cgaccggtat cgccctgatc acagtggatg gcggcggcga gaatgaaatc
     6661 atcgtcattt cgggcagcaa catgaagctt tcgcccgcgg atgtggagag cagactcgca
     6721 cttttcaacc gggtgggcgt ggtgctggca cagctcgaga tcccactcga gacggttgcc
     6781 aggacggcag agcttgctgc ggccgccggt gcctgtttta tcctcaatcc cgcaccggcc
     6841 tgcaagttgc ctgtgaagct gctgcagcgg gtcgattttc tcacccccaa ccgtctcgag
     6901 ctggaactgc tcgccggcat gtccattttg gatgacatcg agtcggtccg gtgcgccgcc
     6961 caacggcttc tggaccaggg cgtcggcaat gtgctggtca ccctcggtga gcgcggctcg
     7021 ctttgggtca ccccggggga ggccaggctg ttcgccgccc ggcgggtcca ggctgtcgac
     7081 accaccgcgg ctggggatgc cttcaacggg gccttcgcct tcgccctctc gcggggtgag
     7141 acggtgccgc aggccattca tttcgccaac cgcgtggcgg ccttctcggt cacgcggatg
     7201 ggcgcacaga gcagcatgcc gacagcggcc gacatcgaag agtgggtgta acgggacaaa
     7261 cggacttgat gccgtcgagc ccggcgccgg cgccactttg tccgccggcc gggcaagtat
     7321 ggatgcatta aacacaggag tgaataatga aaaaacaggg gatactcaat gcgcagctct
     7381 cgtatctggt tgcgcgcatg ggccataccg accggcttgt catctgtgac tctggcctgc
     7441 caattccccc ggaggctgag gttgtggacc tggccctgac ctggaacatt cccgcctttc
     7501 ttgataccct caggacggtg ctgggagaac tgcacatcga gggcgcggtt gtcgctgcgg
     7561 agatggagca ggtcagcaat accctttaca aacagaccct gcagcttctc cccggcatcc
     7621 ccgtcgagaa agtggcccat gagcagttca aggagttgac ccgtgcgccc ggaacgatcg
     7681 ccttcgtccg caccggcgag gcaacctcct acgccaatat catgctcgtt tcaggcgtga
     7741 attttaacca ctcataataa aggaccttca atgagaaaag agtacatcgc tgcactcctg
     7801 ctcctggcca tggctgcatt tgcggcctgc ggagacaccg ccggaagccg ctatgaccgg
     7861 ctggtcaggc ctgcagatgc cacctactat cacgtccctg tcggcctctg tgaagactat
     7921 cctgaagaga gcaccaccct cacaggcatt cgcaaggaca tggaattcct caaggagagc
     7981 aaggtcgatc tgctcagaat ctccttcggt tgggatgcta tcgaggcgga aaaggacaaa
     8041 tatgactggc ttttctggga tgattttgtc aaaatggctg tcgaggagta tggcatcacc
     8101 ctcatacctt acatctgtta tatgccgaga tggaattcaa ccaaaccgga ggataacaac
     8161 tggttctgga actatccccc tgttgatccc cagcaattcg gcgaattcat caagctgctg
     8221 gtgaaccgct acaaaaaata tatcaagacc tgggaactct ggaacgagcc tgatatctgg
     8281 gtctactggc agggatcgcc agagcagttc gcagagatga tcaggattgg cgccagagcg
     8341 gtcaaggagg ctgatcccga cgccagagtc gtgctcggcg gagtcgctta cagcgaagag
     8401 ttcgtcgcca agatgttccg ggatttcggc ctcagcccct acatcgatat cgtcaatatg
     8461 cacaactatt tcgagacctg gagcgagcgg ccggtcgagg atatcgatga ccacattctc
     8521 aagatgaccg atgtgatccg gcaatatggc aacaggcagc cgctctggat ggccgaggtc
     8581 ggctacagca ccttccgtca gggcagcaag atatcgagcg aatataccgc caattacgac
     8641 tatgaacata ccccggaata ccaggcggtg gaccttttca aacgactcgc cctgattgtc
     8701 tcgaccgaaa aggtctcggc cgtggcctgg tacgagattt ccgatctgcc cccctcggac
     8761 gaggtcatcg gggatgaata caacaatcgc cacctcggag tcgcctttgt aaattatgtg
     8821 cccaagccgg cccgcaaggc cctgatgttt ttcaacagcc ttttcggcga cgaatacagg
     8881 aacatcaccg ggcaggcggc gatcacccgg ccggagaatt ccgacagcca tgccctggtc
     8941 tttgagaaag tggatggcag tgtggtcatc gtcagctggc tgcagactgc tctccccggc
     9001 aaacgcagcg ccgacaccag cggtacagtc agggatgaac gccgggagag catcagcgtc
     9061 tccctgccga tggcgctgca aggaagcgcc cgactctatg atcatctcgg tgaaggaaaa
     9121 gcgcttgaaa acatccgccg cgagggcggc atgaccctga tccctgagat tgtcctggag
     9181 ggtggtgcgg tctgcatcat ccatatcgaa aagtagggcg tcagcccgat cctgaggaga
     9241 agcgatgaag agatctgaaa tcaatcattt gcagagggcg gccatggagt ttttcggccg
     9301 ccactgtttc catctgcccc cctgggcgcg ctgggggaaa aaggagtgga tggagaacag
     9361 agaggcagct ggcgagatct ttaacaacaa gctggggtgg gacctgacgg attttggctc
     9421 cggcgatttt tacacgatcg ggctgctcct cttcaccatc cgtaacggct ccccgggaga
     9481 ccctcagggc aaggggtatg cagaaaaggt gatgatcgtg cgcgagggcc aggtgacacc
     9541 ctggcatttc cactggagca aaatggagga tatcatcaat cggggcggcg gcaatctcgt
     9601 catcgaactc gccaacagca ctcctgatga aaagctggcc ggcggtaggg tgcaggtcca
     9661 ggtcgacggc ctcacccgtt ccgtcgaggc gggggggaag attgttctga ctccgggcga
     9721 gagcatctcc ctgccagcct atctttatca caagttctac ggagaggcgg gcaagggcac
     9781 ggtgatggtc ggggaggttt ccatggttaa tgacgacgac aaggacaacc gttttctcga
     9841 tccgatcgga cgtttcccgg tgatcgacga ggacgaggaa cccctctatt acctgtgcaa
     9901 cgagtacccg gtctgacagt gactatcctg gagtcgaata tgatcattct gcgaccgaaa
     9961 caggtcaccc ccgccaactt tgcccgcttc ggcagcgtgg tcaccgcacc gcagacggcg
    10021 ccgacctcgc aggcggcgga ttataaattc tggagcgatc tggcccacta ccgcattgat
    10081 ggtgagaccg agatcggcat ctgtacagtc taccatatgc cggtctccat catcacggag
    10141 gtggaacggc atgagcacac cccggagata ctgatcccta tcaatgcgcc cttcgctctg
    10201 ccgctgctgc gtgatggcga cagtgtcgaa caggccgagt tgtttcgtgt cggaatcggc
    10261 gaggccgtcg tcatcgacgc agggatctgg cacggtccct gcctgccgat aggctgtgcc
    10321 aactcctcct attttgttat ctttgccaga aggacacccc atgcggatgt ccgcaaaaag
    10381 gagatttccc cgatcgagat tatgcaaaag ggacaggacc gcggatgatc ctctccaatt
    10441 caaaaacagc gtttattttc gcagatcagc ccgaccagat cagcgagatc aaactggccg
    10501 acgggacccc ggtcggcagc acgagccggc aggccattct catccgcact ccggcagctg
    10561 tgtcggaacc ggttctgctc tcccgcgtca gctcatccgc ttccgggcct gccagcgctg
    10621 aactgctctt ccaggatgag agcggagcct ggcgtgcgga actgcagatt catgccgccg
    10681 aacagggcct gcgcttccgt ttgcaggcct acgcgccgga gccgatctgg ctggtcgagt
    10741 ggcgcctgtc cggacttcaa ttcgacgagg tcatcatccc cgccctcggc ggccaagctc
    10801 tgagcggggc gatgccggct gaaaccaccc tgagctataa atatcccttc tggtggaatg
    10861 cccaatttgt cgtcggcaaa aaaggcggtg ggggactctg gcttttctca agggatgaaa
    10921 aacccgacct caagctgctg cgcgtcggcc ggtcgagcga cggttttgcc tggacctatg
    10981 gctgtgaagc ccggccgccc tttccgtgca cccttgaggc ggagtggtat cttgacggca
    11041 taaacggcgg ctgggaggtt gcagtcgaac gccaccagcg ttggctcgag gaggcctttg
    11101 atctgcgccc cctgcagcag aaagcctcct ttcccgcctg ggcacgcgag atcaatttcg
    11161 tgctcgagct gtggggagcg cggcgagatt ccatcgcccc catgcacacc ttcgcccaaa
    11221 tggtcgagcg gctgcaaacc tggcggggaa tgcatgatcc gcgccagacc ctggtctatc
    11281 tccccggctt cgccatgcat ggtatcgact cgcatgcccc ggattatcat cccagcgcgc
    11341 agtgcggtgg agctgcggaa tttgcccgcc tcatcgaggc cgctcacagc tggggattca
    11401 gggtgatgat tcacaccaac gtcctggcca tgaccttcaa ccatccccac ttcccccgct
    11461 tccgggaaca tcaggtgatc gattgtttcg ggcggcccca gggatgggga ctggatatgg
    11521 acggcgactg gctggcagaa ccctacttcg cctacatcaa tcccggtgtc agggcgtggg
    11581 gcgacctgat ggtcgatgtc atcggagatc tcgtccgtcg cttcgggatc gacggagtat
    11641 ttctcgatca aaccctgctc gccttcaatg tcagccgtgg acccaatttc atcgaaggca
    11701 tgcgcaccca tattcgacgc ctgcaggagg cgatgccgga gattctcttc gcgggtgaag
    11761 ggatgcatga acatctgctc aatcccctgc cgatggccca aattcatggt atcgacagta
    11821 tcaccgaagt gcacggcatg gaaggacagc tctcctggcg ctccgcccac ccggtctcga
    11881 cagcgctttt cagtccctac tgccgcttta tggcgcatct gctcaccagg catccgtccc
    11941 atcccatgtt tacctttcag gagagcgcct atgcaaaatt gcatgtcatc cctgccctct
    12001 gcctttacag cgccgctcag gccatggatt tgccggaggt gaaagagatg atcgctcgcg
    12061 cacaacagct gacattatcg gatggatttc gatgattccc ggtttgataa ggagaaccaa
    12121 tcatgaagct tggcttttta accgcctgtc tgcctgacat cgccctggcg gatctgctgc
    12181 agtgggcgca ggagaatcag tttcaagccc tcgaactggc ggcctggccg gtggcctcgg
    12241 accgcgacta tcaggcgcgg cagatcgatg ccgcgagaat gacaccggct gaggccggtc
    12301 gggttacgga gctcttccgg gagcatgatc tggaaatctc ggccctggcc tattatgata
    12361 acaatctgca tcccgatctg gcgcagcgtg agggttatca cgaccatctc cgcagggtga
    12421 tcgatacggc cgcactgctg caggtcggat tggtcgggac cttcgtcggc gcccggccgg
    12481 atctctcacc ggccgaaaat atcagggaga ttggcggggt ctttcgcggg ctgaccgcct
    12541 atgctgcgga caagggggtg cggatcatga tcgaaaactg ccccatggag aactgggtcc
    12601 ggttcggctt ccccggcaac tatgcctttt cacccgaact ctggagtgcc cttttcaatg
    12661 aggtcccgtc cgataatttc ggactaaatc tggatccttc ccatctgcac tggctgggta
    12721 ttgattacct ccgcgcggtg cgggagttcg ccccgaaaat ctttcatgcc catgccaagg
    12781 ataccgagat ccttcccgaa ggccgtcatc atcactccat cttcggcacc cagatcgatc
    12841 cagctccctg gaagtcaggc tggtggcgct accggctgcc cggactgggt gagatcgact
    12901 ggcgggcatt catatccgca ctgcaggaaa acggctacga ttatgtcctc tccatcgaac
    12961 atgaggatcc ggtctgggag gggagcgagg aaaaaatcaa gcgggggctg gtgctcggac
    13021 gcaaacatct gtcacctttt atcatttgat aaggccgata cttatgcgtc aactcctgaa
    13081 agaagaatat atccctttcg gcagccagta ttaccgcgcc cccttgccac acgccgggga
    13141 gtgggagcgg gacctcgatc ggatggccgc cctgggcttc aataccgtca agttttgggt
    13201 tcagtggcgc tggaaccacc caacggagga cgaattccgg tttgaggaca tcgaccggct
    13261 tatggaactg gctctggcca ggaagctgca cgtgatgctc aacaccatct tcgatgtcgc
    13321 ccccgcctgg atataccgcc tctatccgga tgcatcgatg gtgactctgg acggccgccg
    13381 catcggcccg cagacccagc cccatcgcca gatcggtggg ctcggctact gtttcaacca
    13441 cgacggagtc atcagtcact ttttccggtt tcttgccgcc actgtagagc gctacaagga
    13501 gcatccggcg ctggcgatct ggaatgtcgg cagcgaaccc gaactgacct cgagcatggc
    13561 cgagatgcgt ctctacgccg atgatgccgg ccggatgggc gatatgctct gctactgccc
    13621 gaactgccag gcgaaattca ggctctggct ggccggcaaa tatgactcga tcgatcggct
    13681 caacgaatgc tggaaccgga actatcgcag ctttgatgag gccgaactcc cgatcacccg
    13741 caacactttc aacgacctga tcgactggcg tatgtttttc gtgcacaccc tcggcgagaa
    13801 cgtccgccgc cgcttcgaaa tcgcgcgcga aatcgaccag ggacgccatc ccatcatgtg
    13861 ccaccatgtc ttcgtgcagg gcttcccggt cacctcgacc gccaacgatc cctggaatat
    13921 cgggcagtac ggcgatcttc acggcttcac ccagatggat gacgccatga tgatcgacgt
    13981 cctgcgctcc tgcgcacggg gcaaaccggt gatttcggct gagatgctcg ccctgccggg
    14041 gtataccctc gatctgcccg cgatcatcga cgccaacacg gtgaagcgtt tcatcttcag
    14101 cggggtcgcc ggcaaccaga agggctttat tttctggcag tatcgtccgg agatcctcgg
    14161 ccgcgaagcg ccgacctggg gtctctccgg gcttgacggc gaggaaaccc cctggctcaa
    14221 agcctatgcg gaggcaggac gggtactgca aaagaacgcc acttttctgc tcgatgccgt
    14281 gcctcagcgg gcccgtatcg ccctgctcta ccatccg
//
LOCUS       DAOPWU010000048        14250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4352_length_14250_cov_4.712998, whole genome shotgun sequence.
ACCESSION   DAOPWU010000048 DAOPWU010000000
VERSION     DAOPWU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14250
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4352_length_14250_cov_4.712998"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PKM23_04295"
     CDS             <1..1195
                     /locus_tag="PKM23_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNY90724.1"
                     /translation="HRRTYIGAMPGRIIQGIKNAGSNNPIFMLDEVDKIGQDFRGDPS
                     SALLEVLDPEQNFSFTDHYLDVPFDLSRVMFITTANVLDTIPSALRDRLEVLELPGYI
                     EEEKLQIAVTYLVPRQIAENGLRKKDIKFARSALTRIIRDYTREAGVRNLEREIATVC
                     RKMAKKIAAGESGPYTIKPETVVELLGPRRFYSEVAERTNEPGVAVGLAWTPTGGDVL
                     FIEATQMSGTKGLMLTGQLGDVMKESAQAGLSWIRAHAQELGIKEDFFEKNDLHIHIP
                     AGAIPKDGPSAGVTLATALVSLIQQRPVRSDIAMTGEVTLRGKVLPVGGIKEKVLAAR
                     RAGIRKVIMPAKNRDDLEEIPDHAKKEMTFHFVETLDEVLELALRGGPPKAAGAAGRP
                     DKKKP"
     gene            1192..2640
                     /locus_tag="PKM23_04300"
     CDS             1192..2640
                     /locus_tag="PKM23_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HNY90725.1"
                     /translation="MSGARRVDERYRRMKERLGVLGQEQVLRYWPALDEEGRRGLLNQ
                     LEALDMEFIAAQRERAWPPVEAGAGSLEGFSPMPHIPLPSGPEEERAWERARRHGEEL
                     LADGAVGVLVVAGGQGTRLGYPGAKGKYPVGPVSGRSLFQYHIEKMVAMERRYGRPLP
                     FYVMTSPANHEETIAFFKQNSWFGKDPDTILCFPQRMLPVFDLRGQLLLESPARLSLS
                     PDGHGGVLYALRKHQLLEDMRRRGIRHLFHFQVDNVLVRIADPAYLGFHEETGAEMSA
                     RTVRKRDPWEKLGNIGRQNGICKVIEYSELPDEKKTACTAAGELLFGQGSIAIHLFSL
                     DFLERLLRQKIELPYHIARKKMASVEEQGAELAAGGLNAVKFEQFIFDAFSFAERVLV
                     LETDRRLEFSPIKNREGEDSPATARRDLSELFAGWLEACGIRPRRHADGSLAVPVEIS
                     PLYAMSAAELGAKISSGLDPEREILLEEPTGE"
     gene            2717..5998
                     /locus_tag="PKM23_04305"
     CDS             2717..5998
                     /locus_tag="PKM23_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90726.1"
                     /translation="MMPAADTPSSPVSPRHRRSRLRRLLRFVLFSFLALLFLLFSAVL
                     VLRLLYPPARIKAMINEAAIEKLDRRLSLGKVWLHPLRGFILEEVSLSPYPDSTSGYD
                     LFVLRQFHARQIAVRYSLRDIFKNKLHLREIEIVGPEMEFFVDMLDTTLIDFAALART
                     ELPLTLDLRSLRLRDSRIRVVLADTLTRQEFFIGDLDAVLKDVRLPGGGFLRNDSVLQ
                     ADLAIRCQATPLSLSQSDLRSGSTLRLEALLDLDTRLSLRSYHDLRLALGLQLRELVY
                     EEEAPGSVRRTAFRPRLNLALGLHGDGHIGSAHLDSLILALDGRRWLVAEGAAEGLYG
                     QGRFSARLSHGEIPLDQALSLAQELLPPGRFPDLQWLEEEAALHFSGAEIAGSLDEGF
                     EYQLPLELRHAGLSLNGDSLRLRGLGASLDLRGRLLPGGTERTALTAALVCDGLRLLQ
                     PGSAPLTAGAGSATADMVLNRLMLPEKAHIALRLDDLMGLKAEGECDLSAPASLQDLS
                     GGGTVSLSGFDLGRFPALAVSGKAGALFSFHLDGLDRIAAALEVKSTPLILLIDEEPV
                     HLDELELEMKLQAGTDTSLALIRIDSLGLALNRMVEAAARAEILNRGDRQIRFNFDRL
                     AIRHDAIWAWLPELLREPFAEADLTGTTTFMGGGRIGFNPSGLRYDVDVRMTTAGTSF
                     RAPAQFTLVNGLGIDARLTAANGDGIHALVEAGLDSLRVGKAGGVAFHHNQLTLQLAS
                     KDLSSLIEAKGRLELPDLFTSGSFEASIAELKRAMRIKARFALNQSIADTLRLPQGIR
                     MRGDSDLLVTVEADTALARVAADISTRGVSFFMPDNVYIRDVLTDLHLRQEVDFVNKR
                     LLASPAFRIATPSNAFMDYMAYRQYYRERLPGFSRLRIGRVELKEYAVEDIDLELYAG
                     EGRLEVPALVARLYGGNLGGRMVLDLAGGDLKKAAYGINLTFANVNSDLLLPKGTRDG
                     KYGIINGNMDFQGIGLDPAAGIRLEGQAYITEIGPKVADNLLRSLDPQGVDSSIRTTR
                     LLINRGFKPKLMTFVLRHGYLYPEIIFAQPWYFPMRLSGGKVELARIPLDMFLRSSGQ
                     GPAAR"
     gene            6061..6600
                     /locus_tag="PKM23_04310"
     CDS             6061..6600
                     /locus_tag="PKM23_04310"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNY90727.1"
                     /translation="MFISLALLRCTVRAPELNVTGEKTALENQVLGAYQQIESDSWLI
                     ASARAVGATSSAVVSGSKKEVIDAVQNRKFNKDEVDELKADKVVGENNRGLLQPLAHP
                     RYDGDPEYRLYVDQIIEEENNDRLIIYERVLTINQTSAEAARANEVFAKLNFDNSAPG
                     TMIQNPDGTWVEKPAIRGK"
     gene            6605..>14250
                     /locus_tag="PKM23_04315"
     CDS             6605..>14250
                     /locus_tag="PKM23_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY90728.1"
                     /translation="MKRRSPAYPRGRTPAGPALLRALFCAGLIAAPCARPASAVSAAM
                     QTTAPAARAAAAQPLDLRQVTTHPDIDLSPRLSPDGKYLAYVSRQTYNFDIWVRSTTG
                     SSSRQITFNKADDFYPVWYPDSRSLVFVSQKEDAGGDIWRIRFREVEGHLIPRGEPQK
                     ISSWQGYDGYPTVSPDGKKIAWVSNASGRDEIWFHNDNTGKTSQLTFLGGTHPAWSSR
                     QDLIAFTSFRAGAENGGDIWLINLHGPRPTDGEVDGLWDPREPSLYPLTRGEAAEGFP
                     TWSPDASRLVFLRFDRDCNGDSLLTPADHGALWEMAPLPAPAESPLFSSPLDRLLQPG
                     FNPLMVRSAMPLTSGSDNIMQPWCGGDQRVYFSSDRGGNLDIWSIPLDGSVPRQPGAA
                     AQYRWADEVFPLLERASRWYLGPLCLGYREVEPDEKEQRLLSERLIALRRLVDFFPDS
                     SRYAARALYEMGIVAMLQGDEGAARSFLQLVTAHYAGERETAAYAELALLGLGVQGKS
                     SGSANGLLKHGIEELLLRYDDQPAPAAAARLALGDLYFESGEHTRAFQEYARVQKEYP
                     GERDACAASQLKIGDLFRQFASQEEVVQAYLHVVDLYPDQRQWMLQARDRIFDLLISG
                     RKGSEAFIARYREIVGQYSRFPALAAEAQFRIGNILLRNKEYRAAIREFEAMETLFPE
                     LVDELFTARMSRAEAWLRLGDSGSALALLDQLVKEQQQERPDLAALASDRLVMALLSS
                     ADKLRASGDLQLAAMRYRSAWERDLHNLQAHRSYLECMYYQKKIDQAIEEYRQINILH
                     PGDNILTYALGLAYSYKGTEKAELFNDPDGLDPVYLVNRSSAIIARALSYDYTMVPGY
                     LTIAFNFEMMENYEARQRAKPTPFLARAWNAVKAPFVDLYHRLTFYQERKPARYYERA
                     IHELNKAIVLNDEILEPQLEANLALNLANNYYNLGEFGYEKAYEFYQVKLLYDSSFTD
                     KQREALIYERMGHCALVTEDLEKGPRFLRHAIELYAAMDREPRVLLNTKRLALLYEIG
                     EQYEAAIDHFQQAAEIEKRNNALPELMRSYRSIAYNYLRLDEPADAIVYARRALDLLD
                     SGKIKRIKGESSRMEVGFLGWYFPVPFIDFSTMGMNSLKSFTTEDERALIFSIFADSY
                     QRNKEYSSAISYVEKKVELFHGRKDYRAEATFYNNLGYIHFLRGDYLRAWEYYRRSLG
                     LCEKYAIADGVIRNTLNMGRIASGLHLQRQLGHSPAAEPGLYADSAAVALQGALAFLD
                     NTQLFYNRDRAQLLLQLAEVMLIEARPEGERSGPGALAATMADLERMGRVKGYLDEAL
                     DLSRRYGLERSELASMYALAELYRAGGDYEEAWEEFNRCRKAALRRGEYTLAWRIDVA
                     LGEVLERMSLESRRHYAIQSVPLEFYLEAVELLEAHPAAALGAVAPDMRRTHQQPYLR
                     VIAALVRMGDTRGALTFAERMRAKSYLDLIGREEITFRRERHKIFLGNARFVQDKIDQ
                     ASSAILRARTQGNVASRRTRELKKQLEGYQNEYEEILKQLRAEVPELEGLVRVQPVDL
                     LQVQKNLQQREALVLYQRMPERLLAWTIRAQSVALNEIPLSGEALATALDSWRRAGGL
                     DAATGDTLWNSLLAPVLKLPEEAQRLVIIPDEELFYLPWCAAIHSLPGREVAVSVSSS
                     LTAWYYAGQKRKLQGRRIYMADDPMLAQALAGQGYQVSGPQPGEQQNSFPAQINSMGL
                     AELIHLKAETAWNTIDPLESRIGFSVPGYAPARFAVKEIYANSFPAGLLTLSGTQPLL
                     GVATAEPGLAWERGLHYAGVPSFLLTLWPGERSREAEFYGLYYANLKSMPPAVALAQT
                     QRGMLARGIPFREWGRFQLYGYGGMTEAEEQQFAVEGFAGKVRRGHAAFRLGEWSDAI
                     VFYEEALQMAERQGDAESIALLEERVLEAAINGALWGKAIELQQRRLEAAERAGDVDA
                     VVTGLNNLAYFHTQNGQYEEGVAAKRRYAELAQQHGLDEQEAESLRETGLIFERGGQY
                     DKAMGFFNQAFERYQQLEHPLGMGLTLRDMGRIEFLYRDHYTAAIDLQERALVLLRPL
                     AARAEIIDALHNLGITHEKMGNYQPALRCQQEALALAGEEGEKRLIALSRQYLANVLW
                     KMGDYQTALQHQNAALETFNKLGDDQLLQTAYATRGLIALSLGQPQQALEFEQKALEI
                     AVRTDDRLNQATIHKNLGMIHRSDGRFELARASFEQAMILDSLLVSQRGLAYDYRNLG
                     AIYGLIGRGSEGMFLAGRGLELSRAIRDSRNEAQSLLVLGALQRRFGHPDSARANLEA
                     SAAMAGELFMPDVTWRAQRQLADLHAGVKNRPAQIQALYAALETIESMRARIRVEEYA
                     SGFIDDKLEVYSALIDALVEGGEAGKALEVAERARARSFLDLLGQRPLVFSSPEESRL
                     AASGDSLQREFARAQSEMLYLQARGDALQAQQLRRLGERIQELRSRYAEHLLRVREAN
                     PELSALYQVEPLGQEEIRHLLPEKGAMLVYHLHRDRLTIWLVTRESVRMQRVETIEAA
                     LGEEIDLLRR"
BASE COUNT         2583 a         4686 c         4403 g         2578 t
ORIGIN      
        1 ccaccgccgc acctacatcg gcgccatgcc cggccgcatc atccagggca tcaagaacgc
       61 cggcagcaac aatccgatct tcatgctcga cgaggtcgac aagatcggcc aggactttcg
      121 cggcgatccc tcctcggccc tgctcgaggt gctcgacccg gagcagaact tttccttcac
      181 tgaccattac ctggatgtac cctttgacct ctcccgggtg atgttcatca ccacggccaa
      241 tgtgctcgac accattccct cggccctgcg cgaccgcctg gaggtgctcg agctgcccgg
      301 ctacatcgag gaggaaaagc tgcaaatcgc ggtcacctat ctggtgccgc gtcagatcgc
      361 ggaaaacggc ctgcgcaaaa aggatatcaa gttcgccagg tccgccctca cccgcatcat
      421 ccgcgactat acccgcgagg cgggggtgcg caacctcgag cgcgagatcg cgacggtctg
      481 ccgcaagatg gccaaaaaga tcgccgccgg ggaaagcggt ccctatacca tcaagccgga
      541 gaccgtggtc gagctgctcg ggccgcgccg tttctattcg gaggtggccg agcgcaccaa
      601 cgaaccgggc gtggcggtcg ggctcgcctg gactcccacc ggcggcgatg tcctcttcat
      661 cgaggcgacg cagatgagcg gcaccaaggg gctgatgctg accggccagc tcggcgatgt
      721 catgaaggag tcggcccagg ccgggctctc ctggatccgc gcccacgctc aggaactggg
      781 catcaaagag gattttttcg aaaagaatga tctccatatc cacattccgg ccggcgccat
      841 ccccaaggac ggcccctccg ccggtgtgac cctcgccacc gccctggtct ccctcattca
      901 acagcggccg gtgcgcagcg acatcgccat gaccggcgag gtcaccctgc gcggcaaggt
      961 tctccccgtc ggcggcatca aggagaaggt cctggcggcg cggcgggcgg gcatccgcaa
     1021 ggtcatcatg ccggcgaaga accgggacga tctggaggag atccccgacc acgccaagaa
     1081 ggagatgacc ttccatttcg ttgagaccct ggacgaagtg ctggagctgg ctctgcgcgg
     1141 cggcccccca aaagccgcag gggcggctgg caggcctgat aaaaagaaac catgagcgga
     1201 gcccggcgag tggacgagcg ctatcgacgc atgaaggagc ggctgggggt tctggggcag
     1261 gagcaggtgc tgcgctactg gcccgcactg gacgaggaag gacggcgggg tctgctcaac
     1321 cagctcgaag cactggacat ggaattcatc gccgctcagc gggagcgggc atggccgccg
     1381 gttgaggccg gcgccggcag tctcgaaggc ttttccccca tgccccatat ccccctgccg
     1441 tcaggcccgg aggaggagcg ggcatgggaa cgggcgcgca ggcatggcga ggagctgctg
     1501 gcggatggcg cggtgggggt tctggtcgtg gccggtgggc agggcacacg cctcggctat
     1561 cccggcgcca aggggaaata ccccgtcggc ccggtcagcg gccgcagcct ctttcagtat
     1621 cacatcgaaa agatggtggc catggagcgg aggtacggcc gtcccctgcc cttctacgtc
     1681 atgaccagcc cggcgaatca tgaggagacc attgcttttt tcaaacagaa cagctggttc
     1741 ggcaaggatc ccgacaccat cctctgcttc cctcagcgca tgctgccggt ttttgacctt
     1801 cgcggccagc tcctcctcga atccccggcg cgcctgagcc tctctcccga tggccacggc
     1861 ggggtcctct atgccctccg caaacatcaa ctcctcgagg atatgcgccg gcgcggcatc
     1921 cgtcacctct tccattttca ggtggacaat gtgctggtcc ggatcgccga tcccgcctat
     1981 ctgggctttc acgaggagac cggggcggag atgtccgccc gcacggtccg caaacgcgac
     2041 ccctgggaaa agctgggcaa tatcggccgg cagaacggga tctgcaaggt catcgagtat
     2101 tcagagctgc cggacgagaa aaagaccgcg tgcaccgccg cgggggaact gcttttcggt
     2161 caggggagca tcgccatcca tcttttctcc ctcgattttc tcgaacggct gctgcggcaa
     2221 aagatcgagc tgccctacca cattgcccgc aagaaaatgg ccagcgtcga ggagcaaggg
     2281 gctgagctgg cggccggggg actcaatgcg gtcaagttcg agcagtttat ttttgatgcc
     2341 ttttcatttg cagagcgcgt cctggtgctg gagaccgacc gccgtctaga gttcagtccg
     2401 atcaagaaca gggaggggga ggattccccc gccacagccc ggcgcgatct gagcgaactc
     2461 ttcgccggct ggctggaggc atgtggaatc cggccgcggc gccacgccga cggctccctg
     2521 gccgtgcccg ttgagatcag ccccctctac gccatgtcgg ccgcagaact cggggccaaa
     2581 atatcctctg gcctggatcc ggagcgggag atactgctcg aggagcccac cggggagtga
     2641 tcataccgct tccggctggc catcaagcca ccggcttgcc cgctcaccca gatgcgggcg
     2701 gccaaggatg ataaacatga tgcccgcagc tgataccccc tccagccctg tctccccgcg
     2761 ccaccggcgt tcccggctgc gccgtctgct gcggtttgtc ctcttctcct ttctcgccct
     2821 tctgtttctg ctcttttcgg ctgtcctggt cctgcggctg ctctatcccc cggcgcgaat
     2881 caaagcgatg atcaatgagg cggccattga aaaactggat cgccgcctct cgctgggaaa
     2941 ggtctggttg catcccctgc gcggcttcat ccttgaggag gtctcgctct ccccctatcc
     3001 cgattccacc tccggctatg acctcttcgt cctccgtcaa tttcatgccc gccagatcgc
     3061 cgtccgctac agcctcaggg atattttcaa gaacaagctc cacctgcgcg agattgagat
     3121 tgtcggtccg gagatggagt tttttgtcga catgctcgat accaccctga tcgattttgc
     3181 agccctggcc cgcaccgaac tgcctctcac cctcgatctg cgcagcctgc gtctgcgcga
     3241 cagccggatc agggtcgtcc ttgctgacac cctgacccgg caggagtttt tcatagggga
     3301 tcttgacgcg gtcctgaaag atgtccggct gccggggggc ggttttctcc gcaacgacag
     3361 cgtcctgcag gccgatctgg cgatccgctg ccaggcaacc ccgctctctc tgagccagag
     3421 cgatctgcgt agcggcagta ccctccggct ggaggcgctg cttgacctcg atacccggct
     3481 ttcgctgcgc agctatcatg acctccggtt ggcgctcggt ctccagctcc gggagctcgt
     3541 ctatgaggaa gaggcacccg gatcagtacg gagaaccgcc tttcgccccc ggctcaacct
     3601 ggcgctcggc ctccacggcg acgggcacat cggctccgcg catctcgatt ccctcatcct
     3661 cgccctcgac ggccggagat ggctggtggc tgaaggcgcc gccgagggcc tctatggcca
     3721 aggccgtttt tccgcacgtc tctctcatgg cgagatcccc ctcgatcagg ccctgtcgct
     3781 ggcgcaggag ctgctcccgc ccggccggtt ccctgatctg cagtggctcg aggaggaggc
     3841 cgccctccac ttcagcgggg cggagatcgc gggttcgctc gacgagggct ttgagtatca
     3901 gctccccctc gagctgcgtc atgccggcct ctccctcaat ggcgattccc tccggctgcg
     3961 gggattggga gcctccctcg acctgcgggg ccgtctcctg cccgggggta ctgaaaggac
     4021 cgccctgacg gccgcgctcg tttgtgacgg cctgcgcctg ctgcagccgg ggagcgcacc
     4081 cctgacggcc ggggccggga gcgcgaccgc ggatatggtg ctcaaccgac tcatgctgcc
     4141 cgagaaggcg cacatcgccc ttcgcctcga tgacctgatg ggcctgaagg ccgagggcga
     4201 atgcgacctg agcgccccgg cctcacttca ggatctgagc ggaggcggca ccgtcagcct
     4261 cagcggcttc gatctcgggc gctttcccgc cctcgccgtc tccggcaagg ccggggccct
     4321 cttctccttc catcttgacg gactcgaccg catcgctgcc gctctggagg tgaagagcac
     4381 tcccctgatc ctgctcatcg acgaggagcc ggtgcatctg gacgagctcg agctcgagat
     4441 gaaactgcag gcgggcacgg atacctcgct cgccctgatc cggatcgact cgctcggtct
     4501 ggccctcaac cgcatggtcg aggctgcggc gagggccgaa atcctcaacc ggggggacag
     4561 gcagatccgg ttcaatttcg accgcctggc catccgtcac gatgcgatct gggcctggct
     4621 gcccgagctg ctgcgcgagc cttttgccga ggcggatctc acagggacga ccactttcat
     4681 gggcggaggc cggatcggct tcaatccctc ggggctgcgc tacgatgtcg atgtccgcat
     4741 gacgacggcc gggacctcct tccgggcccc ggcccagttc accctggtga acggtctcgg
     4801 catcgatgcc cgcctcaccg ctgccaacgg agacggcatc cacgccctcg tggaggccgg
     4861 gctggacagc ctgcgcgtcg gcaaggccgg cggggttgcc tttcatcaca accagctcac
     4921 cctgcagctt gcaagcaagg atttgtcctc gctgatcgag gcaaaggggc gccttgaact
     4981 gcccgacctc ttcaccagcg gctcgttcga ggcgagcatc gccgagctga agagggccat
     5041 gcggatcaag gcccgttttg ccctaaatca gtcgattgcc gataccctgc gccttcccca
     5101 gggcatcagg atgcgcggcg acagcgatct gctcgtcacg gtcgaggcgg atacagccct
     5161 ggcccgtgtg gcagccgaca tctcgaccag gggagtcagc tttttcatgc cggacaatgt
     5221 ctacatccgc gatgtcctca ctgatcttca tctccgccag gaggtggatt ttgtcaacaa
     5281 gcggctgctc gccagtccgg ctttccgcat cgcgaccccg agcaatgctt tcatggacta
     5341 tatggcctac cgccaatact atcgcgagcg gctgccgggt ttttcacgtc tccgcattgg
     5401 ccgtgtggag ttgaaagagt atgcggtgga ggatatcgat ctcgaactct acgccggcga
     5461 gggtcggctg gaggtgccgg cgctggtggc gcggctctac ggcggcaatc tgggagggcg
     5521 gatggtgctg gacctcgccg gcggcgacct caaaaaggcc gcctatggca tcaatctcac
     5581 tttcgccaat gtcaattcgg atcttctcct gcccaagggg acgcgtgacg gcaaatacgg
     5641 catcatcaat ggcaacatgg attttcaggg tatcgggctt gaccccgctg cagggatcag
     5701 gctggagggc caggcctata ttacggagat cgggcccaag gtggcggaca atctgctgcg
     5761 ctcgctggat ccccagggtg tcgattcaag catccgcacg acccggctgc tgatcaatcg
     5821 gggtttcaag cccaaactga tgacctttgt gctgcgtcac ggttatctct atccggagat
     5881 tatttttgcg cagccctggt attttcccat gcgcctcagc ggcggcaagg tggagctggc
     5941 gcgcattccc ctcgacatgt tcctcagaag ctccggccag ggcccggcgg cgcggtgaac
     6001 gcgcggtgcg gactcgggag aacgggaggt tttatgcatc gatctatgtg gttgctgatt
     6061 atgttcataa gtttggcgct gctgcgctgc accgtcaggg ccccggagct taatgtaacc
     6121 ggcgaaaaga ccgccctcga aaatcaggta ctcggcgcct atcagcagat cgagagcgat
     6181 tcctggctca ttgcctcggc gcgggcggtc ggcgcgactt cctcagcggt ggtctcgggc
     6241 agcaaaaaag aggtgatcga tgcggtccag aaccgcaagt tcaacaagga tgaggtggat
     6301 gagctcaagg ccgacaaggt ggtcggcgag aacaaccgcg gcttgctgca gccccttgcc
     6361 catccccgct acgacggcga tccggagtat cgcctctatg tcgaccagat catcgaggaa
     6421 gagaacaacg accgcctgat catctatgag cgggtgctga ccatcaacca gaccagcgcc
     6481 gaggcggcca gggccaatga ggtctttgcc aaactcaatt tcgataattc ggcccccggc
     6541 accatgattc agaatccgga tggcacctgg gtggaaaaac ccgcaatccg gggaaagtga
     6601 cgtgatgaaa cgccgctctc ctgcctatcc acggggccgc acccccgcag ggccggccct
     6661 cctccgggcc ctgttctgtg cgggcctgat cgcagccccg tgcgcccgtc cggcctccgc
     6721 ggtgagcgcg gcgatgcaga cgaccgcccc ggccgcaaga gcggcggcag cccagcccct
     6781 cgacctcagg caggtgacca cccatcccga catcgatctc tcgccacgcc tctcaccgga
     6841 cggcaaatac ctcgcctacg tctcccggca gacctacaat ttcgatatct gggtgcgcag
     6901 caccaccggt tccagcagcc ggcaaatcac cttcaacaag gccgacgatt tctatccggt
     6961 ctggtatccg gacagcagat ccctggtttt cgtctcgcag aaggaagatg ccggcggcga
     7021 catctggcgc atccgcttcc gcgaggtaga gggccatctc atccccaggg gcgagcctca
     7081 aaagatcagc agttggcagg gctatgacgg ctatccgacg gtctcgccgg acggcaagaa
     7141 aatcgcctgg gtctccaatg cctccggccg ggacgagatc tggtttcaca acgacaacac
     7201 cggcaagacc agccagctca cctttctcgg aggtacccac ccggcctgga gctccaggca
     7261 ggacctcatc gccttcacct ccttccgcgc cggcgcagag aacggcggcg acatctggct
     7321 gatcaatctg cacggcccca ggcccacgga tggggaggtc gacggtctgt gggatccgcg
     7381 cgagccctcg ctttatcccc tgacccgggg tgaggctgct gagggcttcc cgacctggtc
     7441 gccggatgcc agccggttgg tctttctgcg tttcgacaga gactgcaacg gcgattcact
     7501 gctgacgccc gcagaccatg gtgccctctg ggagatggcc cccctgccag ccccggcgga
     7561 gagccctctc ttctcctcgc cactggaccg cctcctacaa cccggcttca atcccctcat
     7621 ggtgcgcagc gccatgcccc tcaccagtgg cagcgacaac attatgcagc cctggtgcgg
     7681 cggagaccag cgcgtctatt tttcatccga ccgcggcggc aacctcgaca tttggtccat
     7741 ccccctcgac gggtccgtac cgcggcagcc gggcgcggcc gcgcagtacc gctgggcgga
     7801 tgaggtgttc cccctcctgg aacgagcctc acgctggtac ctgggacccc tctgtctggg
     7861 ttaccgggag gtcgagcctg acgaaaagga acagcggctc ctcagtgagc ggctcatcgc
     7921 cctgagacgc ctggtcgatt tcttcccgga cagcagccga tacgccgccc gcgccctcta
     7981 tgagatgggc attgtggcaa tgctgcaggg ggatgagggg gcggcccgct ccttcctgca
     8041 gctggtcact gcccattatg cgggcgagcg cgaaaccgct gcctatgccg aactcgccct
     8101 gctggggctg ggcgtacagg gcaagagcag cggcagcgcc aatgggctgc tcaaacacgg
     8161 aatcgaggag ctcctgctgc gctacgacga ccagccggcg ccggccgcgg ccgcccgcct
     8221 ggccctcggc gacctctatt tcgaatcggg ggagcacacc cgcgcctttc aggagtacgc
     8281 ccgcgtgcag aaggagtatc cgggggagcg ggatgcctgc gcggcgagcc agctcaagat
     8341 tggcgacctc ttccggcagt tcgccagcca ggaggaggtg gtccaggcct atcttcacgt
     8401 cgtcgacctc tatcccgacc agcgtcagtg gatgctgcag gcgcgggacc ggattttcga
     8461 tctgctcatt tcaggccgca agggctctga agcctttatc gcccgctacc gcgagatcgt
     8521 cggccagtac agccgctttc cggccctggc ggcggaggcg cagttccgca tcggcaatat
     8581 cctgctccgc aacaaggagt accgcgccgc catccgtgag ttcgaggcga tggagaccct
     8641 ctttccggag cttgtcgacg aactcttcac agcccggatg agccgtgccg aggcctggct
     8701 ccgcctcggc gacagcggca gcgccctcgc cctcctcgac cagctggtca aggagcagca
     8761 gcaggaacgc cccgatctgg ccgccctcgc ctccgaccgg ctggtcatgg ccttgctctc
     8821 ctccgccgac aaattgcgcg ccagcggaga cctccagctg gccgccatgc gctaccgcag
     8881 tgcctgggag agggacctgc acaacctgca ggcccatcgc agctatctgg aatgcatgta
     8941 ttatcagaaa aagatcgatc aggccatcga ggagtaccgc cagatcaaca ttcttcaccc
     9001 cggcgataat atcctcacct acgccctggg tctggcctac agctacaagg gaaccgaaaa
     9061 ggctgaactc tttaacgatc cggacggact cgacccggtt tacctggtca accgttcgag
     9121 cgcgatcatc gcccgcgccc tctcctatga ctataccatg gtcccaggct atctcaccat
     9181 cgccttcaat tttgagatga tggagaacta tgaggcccgg cagcgcgcca aaccgactcc
     9241 cttcctggcc cgggcatgga atgcggtcaa ggcccccttc gtcgatctct accaccgcct
     9301 caccttctac caggagcgta agccggcgcg ctattacgag cgcgccatcc acgaattgaa
     9361 caaggcgatc gtcctcaatg acgaaatcct cgagccgcag ctggaggcca atctcgccct
     9421 caacctcgcc aataactatt acaacctcgg cgagttcggc tatgaaaagg cctatgaatt
     9481 ctatcaggtg aagctgctct acgactcctc cttcaccgac aagcagcgtg aggccctcat
     9541 atacgaacgg atgggccact gcgccctggt gaccgaggac ctggaaaagg ggccgcgctt
     9601 cctccgccac gccatcgagc tctacgctgc gatggacagg gagccccggg ttctgctcaa
     9661 taccaagcgg ctggcgctgc tctacgaaat cggcgaacag tacgaggcgg ctatcgatca
     9721 tttccagcag gcggcggaga tcgaaaagag gaacaatgcc ctgcccgaat tgatgcgcag
     9781 ctatcgcagc atcgcctaca actatctccg tctcgatgag cccgccgatg cgatcgtcta
     9841 cgcccgccgc gccctggatc tgctcgacag cggcaagatc aaaaggatca agggggagtc
     9901 gagccggatg gaggtcgggt tcctcggctg gtatttcccg gtccccttta tcgatttcag
     9961 caccatgggc atgaattcgc tcaagagctt caccaccgag gatgaacgcg ccctgatttt
    10021 ttccatcttc gccgacagct atcagcggaa caaggagtat tccagtgcca tcagctatgt
    10081 cgagaaaaag gtcgagcttt ttcacggccg caaggactat cgcgccgagg caacctttta
    10141 caacaatctc ggctatatcc atttcctcag gggcgattac ctccgcgcct gggagtatta
    10201 ccggcgttcg ctgggcctct gtgaaaaata tgccatcgcc gacggcgtga ttcgcaacac
    10261 cctgaacatg gggcgcatcg ccagcggtct gcatctgcag cgccagctcg gccacagccc
    10321 ggccgcggag cctgggcttt atgccgattc cgctgccgtc gccctgcagg gggccctcgc
    10381 ttttctggat aacacccagc ttttctacaa tcgcgatcgg gcgcagctcc tcctccagct
    10441 ggccgaggtg atgctcattg aggcccggcc ggagggtgag cgttccggtc cgggcgctct
    10501 ggccgccact atggcggatc tggagcggat gggtcgggtg aaaggatacc ttgatgaggc
    10561 cctcgatctc agccgccgtt atggcctgga gaggagtgag ctggcgagca tgtacgccct
    10621 cgctgagctc taccgcgccg gcggggatta tgaggaggcc tgggaggagt tcaaccgctg
    10681 ccgcaaggcc gccctgcgcc gcggcgaata cactctggcc tggcggatcg atgtcgctct
    10741 cggcgaggtg ctcgagcgga tgagtctcga aagccggcgc cattacgcca ttcagagcgt
    10801 ccccctcgaa ttctatctcg aggccgtcga gctgctcgag gcccatcccg ccgccgctct
    10861 cggggcggtg gcccccgaca tgcgccggac ccaccagcag ccctacctgc gggtgatcgc
    10921 ggcgctggtg cggatgggcg ataccagggg cgctctgacc ttcgctgaac ggatgcgcgc
    10981 caagtcctat ctggatctga tcggccggga ggagatcacc tttcgcaggg agcgccacaa
    11041 gatctttctc ggcaatgccc gttttgtgca ggacaagatc gatcaggcca gcagcgccat
    11101 cctgcgcgcc cgcacccagg gcaatgtcgc ctcgcggcgg acccgggagc ttaaaaaaca
    11161 gctcgaaggc tatcaaaatg aatacgagga gatcctcaaa caactccgcg ccgaggtccc
    11221 cgaactggag gggctggtac gcgttcaacc ggtcgatctg ctccaggtac agaaaaatct
    11281 gcaacagcgc gaggccctgg tgctttatca gcgcatgccg gagcggctgt tggcctggac
    11341 gatcagggcg cagagcgtcg cgctcaatga aatacccctc tccggggagg ccctggccac
    11401 agccctggac tcctggcgcc gcgccggcgg actggatgct gccacgggcg ataccctgtg
    11461 gaactccctg ctcgctcccg tcctgaagct gccggaagag gcccagcgcc tggtcatcat
    11521 cccggatgaa gaactttttt acctgccctg gtgcgctgcg atccactccc tgccgggccg
    11581 ggaggtggcc gtgagcgtga gttcgagcct gaccgcctgg tattacgccg ggcaaaaacg
    11641 caagctgcag gggcggagga tctatatggc ggatgatccg atgctggctc aggccctggc
    11701 cggacagggc tatcaggtga gcgggccgca gccgggggag cagcagaaca gcttcccggc
    11761 gcagatcaac agcatggggt tggcggaatt gatccacctc aaggccgaga ccgcctggaa
    11821 taccattgat cccctcgaat ctcgcatcgg attcagcgtg cccggttacg ctccggcccg
    11881 ttttgcagtc aaggagatct acgccaactc gttcccggcc gggctgctga ccctgagcgg
    11941 tacacaaccc ctgctcggag tggccactgc cgagcccggt ctggcgtggg agcgcggcct
    12001 tcactatgcc ggtgtgccct ccttccttct caccctctgg ccgggagaga ggagccgtga
    12061 agcggagttc tacggacttt attacgccaa tctcaaaagc atgcccccgg cggtggcgct
    12121 cgcacagacc cagcggggca tgctggccag gggcattccc ttcagggagt gggggcgctt
    12181 tcagctttac ggctatggcg ggatgaccga ggctgaggag cagcagttcg cggtggaggg
    12241 ctttgccggc aaggtccgcc ggggccatgc cgccttccgg ttgggggagt ggagcgatgc
    12301 catcgtcttt tatgaggagg cgctgcagat ggccgagcgg cagggggatg cggagagcat
    12361 cgccttgctc gaggagcgtg tgctcgaggc ggccatcaac ggagcgctgt ggggcaaggc
    12421 cattgaactc cagcagcgcc gcctcgaggc ggccgaacgc gctggcgatg tcgacgccgt
    12481 ggtgaccggc ctcaataacc tggcctattt ccatacccag aacggccaat atgaggaagg
    12541 ggtggccgcg aagaggcggt atgccgagct ggcccagcag cacgggctgg atgagcagga
    12601 ggcggagtcg ctgcgcgaga ccggcttgat ctttgagcgc ggtggacagt atgacaaggc
    12661 gatgggcttt ttcaaccagg ccttcgagcg ctatcagcag ctcgaacatc ctctgggcat
    12721 ggggctcacc ctgcgcgata tggggcgcat cgagttcctc tatcgggacc actacaccgc
    12781 ggccatcgat ctgcaggagc gtgcgctcgt tctcctgcga cccctggcgg cccgcgcgga
    12841 gatcatcgat gctctccaca atctgggcat cacccatgaa aagatgggga actaccaacc
    12901 tgcactgcgc tgtcagcagg aggcgctcgc gctcgccggc gaggagggag agaagagact
    12961 gatcgccctc agccggcagt atctggccaa tgtgctgtgg aagatggggg attatcagac
    13021 cgccctccag catcagaacg ccgccctgga gaccttcaac aaactgggcg acgaccagct
    13081 cctgcagacg gcctacgcca cgcgcggatt gatcgccctg agtctgggcc agccgcagca
    13141 ggctctcgaa ttcgaacaga aagccctgga gatcgctgtc cgcactgacg accgcctcaa
    13201 tcaggcgacc atccacaaaa atctggggat gattcaccgt tccgacggcc gcttcgaact
    13261 ggcgcgggcc agcttcgagc aggccatgat cctcgattcc ctgctggttt cacagcgcgg
    13321 actggcctac gattatcgca atctcggcgc gatctacggc ctgatcggca ggggcagcga
    13381 gggcatgttc ctggctggcc gcggacttga gctgagccgc gccatccggg acagccgcaa
    13441 tgaggcccag agcctgctgg tcctcggcgc cctgcagcgc cgcttcggcc atccggactc
    13501 ggcaagggcg aacctggaag cctccgccgc gatggccggg gagctcttca tgcccgacgt
    13561 cacctggcgg gcgcagcgtc agctcgccga tctccatgcc ggggtcaaaa atcgcccggc
    13621 acagatccag gccctctacg ctgctctcga gaccatcgag tcgatgcgcg cccgcatccg
    13681 cgtcgaggag tacgcctcag gttttatcga cgacaagctc gaggtctaca gcgccctgat
    13741 cgatgcgctc gttgagggag gagaggctgg caaggccctc gaggtggctg aacgcgcgcg
    13801 cgcccgcagt tttctcgacc tgctcggcca gcgcccgctg gttttttcct cgccggagga
    13861 gagccggctc gcggcgtccg gggacagcct gcagcgcgag ttcgcgcggg cccagagcga
    13921 gatgctctat ctccaggccc gcggtgatgc cctgcaggca cagcagctgc gacggcttgg
    13981 ggagaggatc caggagctgc gcagccgcta cgcggaacac ctcctgcggg tgcgtgaggc
    14041 caatcccgag ctgtcggcgc tctatcaggt cgaacctctg gggcaggagg aaatccgcca
    14101 tctgctcccg gagaaggggg cgatgctggt ctatcacctg catcgcgacc gtctcacgat
    14161 ctggctggtc acccgggaat ccgtccggat gcagcgggtt gagaccatcg aggctgcgct
    14221 cggggaggag atcgatctgc tccgccgcag
//
LOCUS       DAOPWU010000049        14214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4362_length_14214_cov_4.549262, whole genome shotgun sequence.
ACCESSION   DAOPWU010000049 DAOPWU010000000
VERSION     DAOPWU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4362_length_14214_cov_4.549262"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1587
                     /locus_tag="PKM23_04320"
     CDS             235..1587
                     /locus_tag="PKM23_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY90729.1"
                     /translation="MWNPFRFFAAGPDAPRLTDQAEIDRLYRRRRLSVMLAITLTYGI
                     AYTCRLGLSVVKKPLIDGNIFTAEQLGLIGSGIFYGYAFGKLVNGFLADHANVKKFLA
                     TGILMSALINIAMGWSTLLWVWVALWALNGWFQGFGSPAGIVALSHWFSNRERGRYYG
                     IWSTAHSIGEGLTFVGSAALVAFFGWQAGFIGPGLLCVLVAVAAYLFVSDRPQTLGLP
                     SVAEWRNDFATGPQAAAAAAKSTWQAQISILKMPSIWILALASATMYMTRYGMNSWGM
                     LFLQEARGFSDLQAGGLLAMNPVAGIAGCAAYGFISDKLFKARRPPVNLICAVMEIAS
                     LLVIFFMPGNNPVWLTIAFLAYGFSINGLVTALGGLFAVDISPKKAAGAAMGFIGVFS
                     YLGAAIQERISGRLIEAGTTMVDGVRHYDFSSAIWFWLGSSVASLILAALLWRVKMRD
                     "
     gene            1610..2512
                     /locus_tag="PKM23_04325"
     CDS             1610..2512
                     /locus_tag="PKM23_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNY90730.1"
                     /translation="MNETRPYDIVLLGNYTKDTIISKTGTRQVDGGGFNYGAHVARMM
                     GLRTAAVTRLARSDVHVVDALTDIGVDAYPTWTPWSTDMRLYYPTDNVDNRILSVTHT
                     AGSFTPDQFTDLEARAFLINASARGEVDLDVISYLRRKKGLLSADAQGFVRIINGEGV
                     LEFDSWEAKSEVLPYFDILKTDAVEAKSMTGEEDIHKAARLLAGLGPKEVVLSHRDGL
                     LVLAGGESFEAPFLPEPLVGRSGRGDTCIAAYVCKRLTAPPAEATVWAAAVTSLKMEA
                     EGPIKRSVTDVETKIARKYNRSGA"
     gene            2609..3709
                     /locus_tag="PKM23_04330"
     CDS             2609..3709
                     /locus_tag="PKM23_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HNY90731.1"
                     /translation="MKVTSGRPHFYHFLAALMLLFSLFSLDCSRCKLCSADKPLKPVI
                     VTEPVQFDSDDPAIWIHPGDPAQSLVIGTDKEANGGLYVFDLNGKIIPEKCVRPLQRP
                     NNVDVEYGLILGGRPVDIAVTTERLLNRIRIFSLPDMRPVDGGGIEVFAGETLRDPMG
                     IALYKRPADGRIYAIVGRKDGPTDGRYLWQYLLEDDGSGTVKATKVREFGLYSGKKEI
                     EAIAVDDELGYVYYSDEQIGVRKYLADPDQAGANNELAFFARTGFAGDLEGISIYTLP
                     GGRGYILVSDQQANKFHIFKREGEPGQPHQHTLVKVIKTSTSESDGSEVTSVALNQTY
                     PHGLFVAMSDNKTFHYYDWSDIAGTELEMTGR"
     gene            3877..6681
                     /locus_tag="PKM23_04335"
     CDS             3877..6681
                     /locus_tag="PKM23_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY90732.1"
                     /translation="MRTRSRGWGLANFNGALVIFLALMLAATALMAQSGRITGKVVDA
                     KSGAFLPGANVLVQGTQLGAASDRQGRYTIENVPAGAHTLVVSYLGYERYTGDVTVTA
                     GADVKRDITLQISYIEGGEVVVNGLRVGQIKALSQQRTAAKIENVVDSEQMQRFPDVN
                     SAEVVQRIPGVAVERDQGEGRYVLVRGMSAQLSSVTINGEKIPSPQGDVRYVALDAIA
                     ADQLAGIEVTKAITPDMDANAIGGSVNLRTKSALDYPGRVFSLTAGSGYDNLRGKPIL
                     QGGVTYGSHFGEAQNIGLMFSASFQRSQRGSDNNEMEWGGADLADGSEADFALQNMEM
                     RDYAFIRDRLTLSGTADYLLKPGHKFFLSGLFSRYDDSESRRALVILPEDGEYQTATS
                     ITEGKIEAAFRNRDQNQTIYNIAAGGEHQFSKVKLDYRLSYSYAAEDEPKHMEAGFEM
                     DEDPDFELDLSDPDIPKWTTNLGAGYEYDPGHFTFDKLEWHDNLTTDKDINGQFNLEI
                     PYMLGVNPGTFKLGAKATLKNKNRNENIWEYGWEGDDDVLMTDFVGDYKNEGFLDNAY
                     RQPPAFDPDKSWAFFDANQGNLLEGEILYEDSDGATYDATEDVYAFYGMTTLNFGNLM
                     ALAGFRDEMTKNDYTGHEVVFNEEGDYESTTEITKTSDYNNFLPMLHLRYRVTPNTNL
                     RAALTTGLARPNYEALVPFRIINREDEEMSIGNPDLKPTTSLNLDLLAEHYFQGIGLL
                     SGGVFHKQLKKTMYPSQYELEEGAYEGYEVEQIVQGDDATLTGLELNWQQQLTFLPGW
                     MSGFGVYANYTWTSSDASIVFDGEERTGIPLAGQSANIANFAISYEKGGFTGRVGLNY
                     HGKFLYELGDSEDEDVYYDNHIQWDLAASQQITRNVQFYLQAINLNNEPMRYYFGDTL
                     RPIQREFYSWWMHAGFKFEM"
     gene            6924..9107
                     /locus_tag="PKM23_04340"
     CDS             6924..9107
                     /locus_tag="PKM23_04340"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNY90733.1"
                     /translation="MNPVIPGDHPDCTLTRIGNDYYTAGSSFNPTPVIYHSTDLVHWE
                     AIAQPVSATWSGYEDKPGGGCWGGHLVYYDHTCWYFFSRGNTMHFTKAPDPKGPWSMP
                     VSVKNPASLPYGLGYDNSLFIDDDNKWYLVVKNGKPNNGIVELGRDGQPTGVVYDLKW
                     LNPDPLYPYSWAEGPVMWKHGGYYYYSFARDLGGGQKVMRSRTLTAEASAWELPGDFF
                     NENDPLKGGSLFENPNHASPVVMLDDSTSWIVHPLYAKGEWKGQGRQGLLNQVRYNAG
                     GKPVADYPVNQPFTAPRLSGSGIPWMVPKSDFFTSAGLHPEWSFLGYTPASKSSLSER
                     PGWLRLSAKSRHKAMTVIKNDAEHNYSLITRLDFAPGSTADEAGLWIVRGDETMSVKL
                     ASSLSSAGKRMINFTFLSAKFETENSIGDTLWLKLLRINHSISGWFSGDGVQWTEVGQ
                     AVDISQIDSFSDFTTFTGTRQGLYVAGSAAAWFDCYIYRDAYSPILADWPCNQFGTTR
                     SYNSPGYVLDNIHPNDWALYAGVEFGQDEYNRVPDSLKVVASCITTGSRIEVWIDSLD
                     TGRKIADCPINHTGGLRTFQTFASAVAPISGRHDLYLKFTGTETGKLFQLRLFQFTAV
                     MDTMTSIQEQPHSARPEQFRLEQNYPNPFNPATTIEYYLPRSGRTKLSIFNIKGEEIA
                     ILVDGSLPAGTHQIRWDARDIAAGLYLYRLEAGDFMQSKKLAVLR"
     gene            complement(9197..10168)
                     /locus_tag="PKM23_04345"
     CDS             complement(9197..10168)
                     /locus_tag="PKM23_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNY90734.1"
                     /translation="MHHYKELGLVNTREMFAKAMAGKYAIPAYNFNNMEQLQAIITAC
                     IETKSPVILQVSSGARKYANQILLRYMAMGAMEIIKDAGVTLPVALHLDHGDTYELCV
                     SCIESGFSSVMIDGSAHSFADNMALTKKVVEYAHSHDVTVEGELGVLAGIEDEVSHEA
                     SHYTNPDDVEEFVAKTGVDSLAISIGTSHGAYKFKVKPGEEVPPLRFDILEEVERRIP
                     GFPIVLHGASSVMPEYVEMINQYGGKMENAAGIPEEQLRRAAASSVCKINIDSDGRLA
                     MTAKIREVFATKPAEFDPRKYLGPARDALVGMYKHKNEHVLGSAGKA"
     gene            complement(10378..13236)
                     /locus_tag="PKM23_04350"
     CDS             complement(10378..13236)
                     /locus_tag="PKM23_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNY90735.1"
                     /translation="MHRDNTVPSFIALVALFILTISGPAFSQIYLDPNAAVTDRVEDL
                     LSRMTLAEKIGQMTQANLTNLASDAEVAGLYLGSILSGGGAVPDPNTPAVWADTYDRL
                     QAQALSTRLAIPILYGIDAVHGHNNVKGAVIFPHNIGLGAARNPELVRRAAEITALEV
                     AGTGIDWTFAPCVSAVRDERWGRTYEGFGESSELAELLGAAQVRGFQGDSLGRPDRVL
                     ACAKHYLGDGGTTGGVNEGNTECDEATLRRLFLPAYARAIEAGVATIMPSYSSWNGEK
                     MHSHTYLLTTVLKGELGFQGFVVSDWDGITRLPGSDERAKIVKVVNAGVDMGMVPTSY
                     RDFIRHLTAAVQAGEVSMARIDDAVRRILAVKFRMGLFEHFYTDRALTAEIGSAAHRE
                     VARACVRESLVLLAKKEGILPLSRSAARIHVAGNGADDIGNQCGGWTISWQGSSGDIT
                     GGTTILAALRAEAPGVQFTYSLDGSGAAGADLGIAVIGETPYAEGQGDRSDLHLPAKV
                     IEPVRALKNAGLKTVVILLSGRPMIIDNILPYADAIIAAWLPGSEGSGVADVLFGGHP
                     FTGTLPHSWPRAMAQIPINIGDDPYRPLYPYGHGITSLADSDPGTPPHFFAASVSAGG
                     DTLFAGFDKAMADPVAAPTAEWTVLVNWGVTGVTALSRHPKDPTILCLALAKPVLKHD
                     VVQLQYTGSNARSADNGLLAPFESQPVYNYRDEVRGKDPVPGRVQAEDYIRMQGVQTE
                     STSDVGGGENVGYIDSGDYMIYQVEVAESGSYDLDFRVAAQSGAGQIKVIDAVTNKQL
                     ATIDLPVTGGWQIWQTATVTVLLNKGPATLRMQASRGGFNLNWIEFYLLTGVESRPVN
                     APGRFLLAANYPNPFNAATTITCSLPDGNKGGRLSLTISDARGRIVRRLEALPAGAEH
                     RFTWDGRDESGLPVPSGLYFYTALFGDAKQSRKLLLLR"
     gene            13591..>14214
                     /locus_tag="PKM23_04355"
     CDS             13591..>14214
                     /locus_tag="PKM23_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90736.1"
                     /translation="MKSFWIVFLIPAVLCAGGRTVSFSAINPVTYAQVELDSVRVENL
                     TLGRDTTLAGVERIELESWTGIARAAALPLHFDLTGNYPNGFAFATRFSTALPHGGLL
                     ELELFNILGQRLTARAFNLPPGWHAFALEGSALANGVYFLRAASGTQQQVIKMVKIGP
                     SVQGNLQILYQGTGGRPAGMALAKTAEADLYRFTAFAAGYVDAVVEQR"
BASE COUNT         2802 a         4327 c         4291 g         2794 t
ORIGIN      
        1 cgatatgggc cccttcttca ccgagactat ccccctggag cagggtggaa gtgcctttgc
       61 caagctgggg ctggacctcc ccaccctgag ccatgtgccc aagcaggcga tgaagatcac
      121 cctcaagccc tgacccgccg gtctgtgccg ggtgactcgc tgcgtggagc gcccggccgc
      181 accgggatgg gggagggtaa agtccattca tgagttagtc gaggatatca tcccatgtgg
      241 aatccctttc gtttttttgc cgccggaccg gatgccccgc gcctgacgga ccaggcggag
      301 atcgaccggc tctaccgccg ccgccgtctc tctgtcatgc tcgccattac cctcacctac
      361 ggcatcgctt atacctgccg gctggggttg tcggtggtga aaaagcccct catcgacggc
      421 aatattttca ccgccgagca gctcggcctc atcggatcag gcatttttta cggctatgcc
      481 tttggcaagc tggtcaacgg ttttctcgcc gaccacgcca acgtcaagaa attcctcgcc
      541 accggcattc tcatgtccgc cctgatcaat atcgccatgg gttggtcgac cctgctctgg
      601 gtctgggtcg ccctctgggc cctgaacggc tggttccagg gctttgggtc gccggcgggt
      661 atagtcgcgc tttcgcattg gttcagcaat cgcgagcgcg gccgctacta tggcatctgg
      721 agcaccgccc actccatcgg cgaggggctg acctttgtcg gttcggcggc cctggtcgcc
      781 ttttttggct ggcaggcggg attcatcggg ccgggtttgc tctgcgtcct ggtcgctgtc
      841 gccgcctatc tctttgtctc ggaccgtccg cagaccctgg ggctgccctc agtggccgag
      901 tggcgcaatg atttcgccac cgggccgcag gcggccgcgg cggcggcgaa gagcacatgg
      961 caggcccaga tctcgatcct gaaaatgccc tcgatctgga ttctcgccct cgccagcgcc
     1021 accatgtata tgacccgcta cggcatgaac agctggggca tgctctttct ccaggaggcg
     1081 cggggctttt ccgatctcca ggccgggggg ctgctggcga tgaatccggt cgccggcatc
     1141 gccggctgcg ccgcctacgg ctttatctcc gacaagctct tcaaggcgag gcgtccgccc
     1201 gtcaatctga tctgcgccgt gatggagatc gcctccctgc tggtgatctt tttcatgccc
     1261 ggcaacaatc ctgtctggct gaccattgcc tttctcgcct acggtttctc catcaacggc
     1321 ctggtcaccg ccctgggcgg cctcttcgcc gtcgatatct cccccaaaaa agcggcgggc
     1381 gcggccatgg gctttatcgg ggtcttcagc tatctcggcg ccgccatcca ggagcggatc
     1441 agcggccgcc tgatcgaagc gggcaccacc atggttgacg gcgtgcgcca ttatgatttc
     1501 tccagcgcca tctggttctg gctcggcagc tcggtcgctt ccctgatcct ggcggccttg
     1561 ctctggcggg tgaaaatgag agattgaaca ctacacagga aagaacagta tgaacgaaac
     1621 cagaccctac gacatcgtcc tgctcggcaa ttataccaag gacaccatca tctccaaaac
     1681 cggcacccgc caggtcgacg gcgggggatt caattacggc gcccacgtgg cgcggatgat
     1741 ggggctgagg accgcggcag tgacccgcct ggcccgcagt gacgtccatg tcgtcgacgc
     1801 cctcactgac atcggcgtcg acgcctaccc cacctggacc ccctggtcca ccgacatgcg
     1861 cctctactat cccaccgaca acgtcgacaa ccgcatcctc tcggtcaccc ataccgccgg
     1921 ctccttcacc cccgaccagt tcactgatct cgaggcgagg gcttttctca tcaacgcctc
     1981 ggcccgcggc gaggtcgatc tggatgtgat cagctacctg cgcaggaaga agggcctgct
     2041 ctcggctgat gcccagggct ttgtgcgcat catcaacggg gagggagtgc tcgagttcga
     2101 cagctgggag gcgaagagcg aagtgctgcc ctatttcgac atcctcaaga ctgatgcggt
     2161 ggaagcgaag agcatgaccg gcgaggagga tatccacaag gcggcgcgtc tgcttgccgg
     2221 cctggggccg aaggaggtgg tcctttcgca tcgtgacggc ctgctcgtgc tggccggagg
     2281 cgagagcttt gaggcgcctt tcctgcccga gcccctggtg ggccgcagcg gccgcggcga
     2341 tacctgcatc gccgcctatg tatgcaaaag gctgaccgcc ccgcctgcgg aggccacggt
     2401 ctgggccgcg gcagtgacca gcctcaagat ggaggctgag ggcccgatca agcgcagcgt
     2461 cacggatgtc gagaccaaaa tcgcgcgtaa atacaaccgg agcggggcct gagaggccgc
     2521 catccactgt aaaggaaagt ttttcttaac aaagaattaa tttgcaggaa atgaatatat
     2581 gtgctaattt aagacattgg aggatagaat gaaggtgaca tccggacgcc cccattttta
     2641 ccacttttta gcagcactca tgctgctctt ctcccttttc tccctcgatt gttcccgctg
     2701 caaactctgt tccgccgaca agcccctcaa accggtgatc gtgaccgagc cggtgcagtt
     2761 cgattccgac gatccggcca tctggatcca tcccggcgac ccggcgcaga gcctggtcat
     2821 tggcaccgac aaggaggcca acggcggcct ctatgttttt gatctcaatg gcaaaatcat
     2881 cccggaaaag tgcgtgcgcc ccctgcagcg gcccaataat gtcgatgtcg agtacggcct
     2941 catactcggc ggccggccag tcgacatcgc tgtcaccacc gagcggttgc tcaaccgcat
     3001 ccgcatcttc agcctgccag acatgcggcc ggtcgacggc ggcggcatcg aggtctttgc
     3061 gggcgaaacc ctgcgtgatc cgatgggcat cgccctctac aaaaggcccg ccgacggccg
     3121 tatctacgcc atcgtcggcc gcaaggacgg ccccaccgac ggccgctacc tctggcagta
     3181 tttgctcgag gacgacggct ccgggacggt caaggcgacc aaggtgcggg aattcggcct
     3241 ctacagtggc aaaaaggaga tcgaagccat cgccgtggat gatgaactgg gctacgtcta
     3301 ttattcggac gagcagatcg gggtgcgcaa gtaccttgcg gaccctgatc aagccggtgc
     3361 aaacaacgaa ctcgccttct tcgcccgcac gggctttgca ggggacctcg agggcatctc
     3421 gatctatacc cttcccggcg gacggggcta tatcctcgtc agcgatcagc aagcgaacaa
     3481 gttccatatc ttcaagcgcg aaggcgaacc cgggcagccc catcagcaca ccctggtcaa
     3541 ggtgatcaaa acctccacca gcgagagcga cggctccgag gtgacctcag ttgccctcaa
     3601 tcagacctac ccgcacggtc tcttcgtagc catgtcggac aacaaaacct tccattacta
     3661 cgactggagc gatatcgccg gcacagaact ggagatgacc ggcaggtagc aaacaaattg
     3721 accctgatga tcacgccgcc cctgtaaagc tgagccgatc cccgcaaggc ggcgtcacat
     3781 cagtaaagca gtacagcgtt ttcccgaaga gattattgct gcacccatat acttaccagc
     3841 catgtgcatc atcaagctaa cccaaaggag gcaggaatga gaacaaggag tagaggatgg
     3901 gggctcgcta atttcaatgg agcgctggtt atttttctgg ctctgatgct ggccgctaca
     3961 gcgctgatgg cgcaatccgg gcgcatcacc ggtaaggtgg tcgacgccaa atccggggcc
     4021 tttctgcccg gcgccaatgt cctggtgcag gggacgcagc tgggtgcggc gagcgaccgc
     4081 cagggccgct acaccatcga aaatgtcccg gccggagcgc atacgctcgt agtcagctac
     4141 ctgggttatg aaaggtacac cggggacgtg acggtcacgg ccggtgccga tgtcaagcgc
     4201 gacatcaccc tccagatcag ctacatcgag ggcggcgagg tcgtcgtcaa tggcctgcgc
     4261 gtcggccaga tcaaggccct cagccagcag cgcacggcag ccaagatcga gaatgtcgtc
     4321 gactctgaac agatgcagcg cttccccgac gtcaactcgg cggaggtggt gcagcgcatc
     4381 ccgggcgtcg ccgtcgaacg cgaccagggc gagggccgct atgtgctcgt acgcggcatg
     4441 tccgcccagc tcagctcggt gaccatcaat ggcgaaaaga tcccctcgcc ccagggcgat
     4501 gtccgctatg tcgcccttga tgctatcgcc gccgaccaac tggccgggat cgaggtcacc
     4561 aaggcgatca ctccggacat ggatgccaac gccattggcg gctcggtcaa cctgcgcacc
     4621 aagagtgccc tcgactatcc tggccgcgtc ttcagcctca ccgccggctc cggctatgac
     4681 aacctgcgcg gcaagccgat ccttcagggc ggcgtcacct acggcagcca tttcggcgag
     4741 gcccagaata tcggcctgat gttcagcgcc agcttccagc gcagccagcg cggctcggac
     4801 aacaacgaga tggagtgggg tggcgccgat ctggccgacg gctccgaggc cgatttcgcc
     4861 cttcagaaca tggagatgcg tgattacgcc ttcatccgcg accgcctgac cctctccggc
     4921 accgccgact atctcctcaa acccggccac aagttcttcc tgagcggcct cttcagccgc
     4981 tacgatgaca gcgaaagccg ccgcgccctg gtgatcctcc ccgaggatgg cgagtatcag
     5041 accgccacct cgattactga gggcaaaatc gaggccgcat tccgcaatcg cgaccagaac
     5101 cagaccatct acaacattgc cgccggcggc gaacaccagt tcagcaaggt caaactcgac
     5161 taccgcctgt cctatagcta tgcggctgag gatgaaccca agcacatgga agccggtttt
     5221 gaaatggacg aggatcctga cttcgaactc gacctttccg accccgacat cccgaaatgg
     5281 accaccaacc tgggcgccgg ttacgagtac gatcccggcc atttcacctt cgacaagctg
     5341 gaatggcatg acaacctcac caccgacaag gacatcaacg gccagttcaa cctcgagatc
     5401 ccctacatgc tcggcgtcaa tcctggcacc ttcaagctcg gcgccaaggc gaccctcaag
     5461 aacaagaacc gcaacgagaa tatctgggaa tacggctggg agggggatga cgacgtcctg
     5521 atgaccgatt tcgtcgggga ctacaagaat gaaggttttc tcgataacgc ctaccgccag
     5581 ccccccgcct tcgaccccga caagagctgg gcctttttcg atgccaacca gggcaacctg
     5641 ctcgaaggtg agatcctcta cgaggactcg gacggcgcca cctatgacgc cactgaggat
     5701 gtctatgcct tttacggcat gactaccctc aatttcggca acctcatggc cctggcaggc
     5761 ttccgcgacg agatgaccaa aaacgattac actggccatg aggtggtttt taacgaggag
     5821 ggagattacg aaagcaccac cgagattacc aagacctcgg attacaacaa ctttttgccc
     5881 atgctccacc tgcgctaccg cgtcaccccc aataccaacc tgcgcgcggc cttgaccacc
     5941 ggactggcgc ggcccaatta cgaagccctc gtgcccttcc gcatcatcaa ccgcgaggat
     6001 gaggagatgt cgatcggcaa tcccgatctc aagccaacca cctcgctgaa cctcgacctc
     6061 ctcgccgagc actacttcca gggcatcggc cttctctccg ggggtgtgtt ccacaaacag
     6121 ctcaagaaga ccatgtaccc ctcgcagtat gagctggagg agggcgctta cgaaggttat
     6181 gaggtcgaac agattgtcca gggcgatgat gccaccctga ccgggcttga gctcaattgg
     6241 cagcagcagc tgaccttcct gcccggctgg atgagcggat tcggtgtcta tgccaactat
     6301 acctggacct cctccgacgc ctcgatcgtc ttcgacggcg aagagcgcac cggcatcccc
     6361 ctcgccgggc agtccgccaa tatagccaac tttgccatca gctacgaaaa gggcggcttc
     6421 accggccgcg tcggcctcaa ctatcacggc aagttcctct atgaactggg cgattcggag
     6481 gatgaggatg tctactatga caaccacatc cagtgggacc tggccgccag ccagcaaatc
     6541 accaggaatg tgcaattcta tctccaggcg atcaatctca acaacgaacc gatgcgctac
     6601 tatttcggcg acaccctgcg gccgatccag cgcgaattct actcctggtg gatgcacgcc
     6661 ggcttcaagt tcgagatgta aggatctggc tgaagcggag gcattaacca atccgctgat
     6721 actgtgggag aatcgcaacc acatcccggg aacttgcccc agggcagtcc cgggatgttt
     6781 tattgtgata cccctgttcc ttcgccatca ggttgtcttt ttagtgacgg gataaaattg
     6841 tggtccgata caggataatt cttgccatgg gcctgctggt gtggagcgct gcaatcgggc
     6901 aatctccatc cttcgggagt tatatgaatc cggtgatccc tggtgatcac cccgattgta
     6961 cgctgacccg gatcggcaac gattactata ccgccggctc ctctttcaac ccgactccgg
     7021 tgatctatca ttcgaccgat ctggtacact gggaggccat cgcccagccg gtgagcgcga
     7081 cgtggtccgg ctacgaagac aaaccgggag gcggctgctg gggcggccat ctggtatact
     7141 acgaccacac ctgctggtat ttcttttcac ggggcaatac catgcatttt acgaaagcgc
     7201 cggatccaaa aggcccctgg agtatgcctg tgagcgtgaa aaatccggca agcctgccct
     7261 acggcctggg atatgacaac tcccttttta tcgacgacga caacaaatgg tatctggtcg
     7321 taaaaaacgg caagcccaat aacggcatcg ttgagttggg cagggacggt cagccgaccg
     7381 gagtggtcta cgatctaaag tggctgaatc ccgatccact ctatccttac agctgggccg
     7441 aagggccggt aatgtggaag catggcggat actattatta ctcgttcgcc agggacctgg
     7501 gcggcggaca gaaagtgatg cggagcagga ccctgaccgc cgaagcctca gcatgggagt
     7561 tgccgggtga ctttttcaac gaaaatgatc ccctgaaagg cggctcactg ttcgaaaatc
     7621 cgaatcatgc ctcacctgtg gttatgctcg acgacagcac ctcgtggatt gtgcacccgc
     7681 tctatgccaa gggggagtgg aaaggccagg gcaggcaggg cctactcaac caggtccgtt
     7741 ataatgccgg cggcaaaccg gtggccgatt atcccgttaa tcaacccttt acggcgccgc
     7801 gattgtccgg cagcggtatc ccctggatgg tgcccaaatc ggattttttc acttcggccg
     7861 gtttgcaccc ggagtggtca ttcctcggct acacccccgc cagcaaatca tccctgagtg
     7921 agcgtcccgg atggctgcgc ctctccgcca aaagcaggca taaagcaatg acggtgatca
     7981 agaacgacgc ggagcacaat tattcgttga tcacccgcct cgactttgcc ccgggttcga
     8041 cagccgatga agccggactc tggatcgtac ggggagatga aaccatgtct gtcaagctcg
     8101 ccagttcgct gtcaagcgcc ggaaaaagga tgatcaactt tacattttta tcggccaaat
     8161 ttgagacaga aaacagcatc ggcgacaccc tgtggctcaa gctgctccgt atcaaccact
     8221 ccatcagcgg ctggttcagc ggggatgggg tgcaatggac cgaggtcggc caggcggtgg
     8281 atatctcaca gatcgactcc ttttccgatt ttacgacctt taccggcacc cggcagggat
     8341 tgtatgtggc gggatcagct gcggcgtggt ttgattgcta tatctaccgg gatgcctact
     8401 cgcccattct ggcggattgg ccctgcaatc aatttggcac aacccgctcc tataacagcc
     8461 cggggtatgt gctggacaac atccatccga atgactgggc gctctatgcg ggtgtcgaat
     8521 tcggccagga cgagtacaac agggtgccgg actccctgaa ggtagtcgct tcctgcatca
     8581 cgaccggcag ccgcatcgag gtctggatag attctctgga taccggccgt aaaatcgccg
     8641 actgccccat caatcacacc ggcggcttga gaacctttca aaccttcgcg tcggcggtgg
     8701 ccccgatctc cggccggcat gatctctatc tcaaattcac cggcacggaa accggcaagc
     8761 tgtttcagct gcgtctgttt cagttcaccg ccgtgatgga caccatgacc tcgatccagg
     8821 agcagccgca ctccgcccgg ccggaacagt tccggctgga gcaaaactat cccaatccct
     8881 tcaatccggc gactaccatt gagtactacc tgccaagaag tggccggact aagctcagta
     8941 tattcaacat caaaggtgag gagattgcga ttctcgtcga tggctctctc ccggctggca
     9001 cgcatcagat ccgctgggat gcgcgcgaca tcgcggccgg gctctatctt tatcggctcg
     9061 aggcggggga ttttatgcag agcaaaaagc tcgcagtgct gaggtgagat ggggagattc
     9121 cacgagggga gagcaaatta ccccggttaa acaaaaaagg cggccatgag ccgccttttt
     9181 tattgctgga tcgggatcac gccttgccgg cgctgccgag cacatgctcg tttttatgct
     9241 tgtacatccc gaccagggcg tcgcgggccg ggccgaggta cttgcgcgga tcgaattcgg
     9301 cgggcttggt cgcaaagacc tcgcggatct tggcggtcat ggccaggcgg ccatcgctgt
     9361 cgatgttgat cttgcagacc gacgaggcgg cggcacggcg cagctgctct tcgggaatgc
     9421 ccgcggcgtt ctccattttg ccgccgtact gattgatcat ctccacatac tcgggcatca
     9481 ccgagctggc cccgtgcagg acgatgggga agccgggaat gcggcgctcg acctcttcga
     9541 ggatgtcgaa acggagaggc ggaacctctt cgcccggttt caccttgaac ttgtaggcgc
     9601 cgtgggaggt gccgatcgag atcgccaggc tgtcgactcc ggttttggcg acgaactctt
     9661 cgacgtcatc gggattggtg tagtggctgg cctcgtggct gacctcatcc tcgatgccgg
     9721 ccaggacgcc gagctcgccc tcgacggtga cgtcatggct gtgggcatac tcaaccactt
     9781 tttttgtcag ggccatatta tccgcgaaag agtgggccga accatcgatc atgaccgagg
     9841 agaagccgga ctcgatgcag gagacgcaga gttcataggt gtcgccatgg tcgaggtgaa
     9901 gggccaccgg cagggtcacg cccgcatcct tgatgatctc catggcgccc atggccatgt
     9961 agcgcagcag gatctggttg gcgtacttgc gcgcgcccga ggagacctgc aaaatcaccg
    10021 gggatttggt ctcgatgcag gccgtgatga tcgcctgcag ctgctccata ttgttaaaat
    10081 tgtaagccgg tatggcgtat ttgccggcca tggctttggc gaacatctcc cgggtgttca
    10141 cgaggccaag ctctttgtag tgatgcatgc gggatctcct ttcgaatgat ctatttaaaa
    10201 tggccatggg tatggcccgg actgccgatc aagtctttat aatagcactt tttttggaga
    10261 aattcaatac ttttcatcag ctctgccctg ccggatgcag cttgtcatcc atggaccagc
    10321 ggatgcaacc tccccgggct tttggcagcg tgctgatgaa ctcctcttgt agaggactca
    10381 gcgcagcagg agcagcttgc ggctctgttt ggcatcccca aagagggcgg tatagaagta
    10441 aagtccgctc ggcacgggga gtccgctctc gtcgcggccg tcccaggtga agcggtgttc
    10501 ggccccggcg gggagggctt cgaggcggcg cacgatgcgg ccgcgggcat cgctgatggt
    10561 gagggagagg cgccccccct tgttcccgtc agggaggctg caggtgatgg tggtggccgc
    10621 attgaaggga ttgggataat tcgcggcgag caggaagcgt ccgggagcgt tgaccgggcg
    10681 ggattcgacg ccggtgagca gataaaattc gatccagttg agattgaatc ctccgcgcga
    10741 ggcctgcatg cgcagggtgg cggggccctt gttgaggagg accgtgacgg tcgccgtctg
    10801 ccagatctgc cagccgccgg tgacagggag gtcgatggtt gcgagctgct tgttggtgac
    10861 ggcgtcaatc accttgatct gcccggctcc gctctgggcg gcgacgcgga aatcgagatc
    10921 atagctgccg ctttcggcca cctcgacctg gtagatcata tagtcgccgc tgtcgatata
    10981 gccgacgttt tcgcccccgc ccacatcgct ggtgctctcg gtctgaaccc cctgcatgcg
    11041 gatgtaatcc tcggcctgga cgcggccggg cacgggatcc ttgccgcgga cctcatcgcg
    11101 atagttgtag accggctggc tttcaaaggg ggcgagcaaa ccgttgtcgg cgctgcgcgc
    11161 atttgagccc gtgtactgaa gctggacgac atcatgtttg agaacgggtt tggccagggc
    11221 gaggcagagt atggtgggat ccttggggtg acgggagagc gcggtcaccc cggtcacccc
    11281 ccagttgacc aggacggtcc attcggcggt gggggctgca accggatcgg ccatcgcctt
    11341 gtcgaatcct gcaaagaggg tgtcgccgcc ggcgctgacc gaggcggcga aaaaatgcgg
    11401 gggggtgccc ggatcggaat cggcgaggct ggtgatgccg tggccgtagg gatagagcgg
    11461 gcggtaggga tcgtcgccga tgttgatggg gatctgggcc atagcgcgcg gccatgaatg
    11521 tggcagtgtg ccggtgaagg ggtggccccc gaagagcacg tctgcgactc cactgccctc
    11581 gctgccgggc agccaggcgg cgatgatcgc gtcagcgtaa gggaggatgt tgtcgatgat
    11641 catcggccgt ccagagagca ggatgaccac ggtctttaag ccggcgttct tgagggcgcg
    11701 caccggttca atcaccttgg ccgggagatg gaggtcgctg cgatcgccct ggccttcggc
    11761 atatggggtc tcgccgatga cggcgatgcc gagatcggct cccgcggcgc ccgagccgtc
    11821 gagggagtag gtgaactgga ccccgggggc ctcagcgcgc agggccgcga ggatggtggt
    11881 gccgccggtg atgtcgccgc tgctgccctg ccagctgatc gtccagccgc cgcactggtt
    11941 gccgatgtcg tcggctccgt tgccggcgac gtggatgcgg gctgcagaac gggagagggg
    12001 caggataccc tcctttttgg cgagcaggac cagggactcg cgcacgcagg cacgggcgac
    12061 ctcgcgatgg gcggcgctgc cgatctccgc ggtgagcgcc cggtcagtgt aaaaatgctc
    12121 aaagagaccc atacggaatt tgacagcgag gatgcggcgc acggcgtcgt cgatgcgcgc
    12181 catcgagacc tcaccggcct gaaccgcagc ggtgagatgg cggatgaaat cgcggtagct
    12241 ggtcggcacc atgcccatgt cgacgccggc gttgaccacc ttgacgattt tggcccgttc
    12301 atcgctgccc ggcagccggg tgatgccgtc ccagtccgag acgacaaagc cctgaaagcc
    12361 gagttcgccc ttgagcacgg tggtgagcag gtaggtgtgg ctgtgcattt tttcaccgtt
    12421 ccagctgctg taggagggca tgatcgtggc aacgccagcc tcgatcgcgc gggcataggc
    12481 gggcagaaaa aggcggcgca atgtcgcctc gtcgcactcg gtgttgccct cgttgacccc
    12541 gcccgtggtg ccgccgtcgc cgaggtagtg cttggcgcag gcgaggacgc ggtcgggccg
    12601 gccgagggag tcaccctgaa agccgcgcac ctgggcagcc cccaggagct ccgccagttc
    12661 ggagctctcg ccaaagccct cataggtgcg gccccagcgc tcgtcacgca ccgcggagac
    12721 gcaaggggcg aaggtccagt cgatgccggt cccggccacc tcgagggcgg tgatctcggc
    12781 ggcgcggcgc accagttcgg gattgcgggc cgcccccagg ccgatgttgt gcggaaagat
    12841 gaccgctccc ttgacgttgt tgtggccatg cacggcgtct atcccgtaaa ggatggggat
    12901 ggccagccgg gtggagaggg cctgggcctg caggcggtcg taggtgtcgg cccagaccgc
    12961 cggggtattg ggatccggca cagcgccgcc gccgctgagt atcgagccga gatagaggcc
    13021 ggcgacctcg gcatcggagg cgagattggt gaggttggcc tgggtcatct ggccgatttt
    13081 ctccgccagg gtcatgcggc tgaggagatc ctcgacccga tcagtcaccg cagcattggg
    13141 atcgagatag atctggctga aagccgggcc ggatatcgtc aggatgaaaa gtgcaacaag
    13201 tgcgatgaaa ctcgggaccg tgttgtctct gtgcatgtca ggtttccttc tgaacgcctg
    13261 gggaccgcat accgggcttt gtattcttcg cggcaaggat actggacttg acgatcaaga
    13321 ccagtaagaa cctttaaaca tagtaaaacc gatcctgcca gtcaacaaaa agtttgccgc
    13381 cggaccggga tgcatggaga gcggctgccc tgtggcgtgg gaacgggtgc acaccgggag
    13441 gcgggcctct ccggctttgc ccttgacatt aactgactga atggataatt taggatatag
    13501 ttcgcgctgt gaagattgcc ggaaacagcg catcgattac ctgcgcaaag gaggagttat
    13561 gggtgaaccc ttggtacatc gggtgccatc atgaaatctt tctggatcgt ttttctcatc
    13621 ccggccgtgc tctgcgccgg cggccggact gtctcatttt cggctatcaa tccggtcacc
    13681 tatgcacagg tggaactgga cagcgtccgg gtcgagaatc tgacccttgg ccgcgatacc
    13741 acccttgcgg gcgtggagcg gatcgagctg gagagttgga ccgggatcgc ccgggccgca
    13801 gccctgcccc tccattttga cctgaccggc aattacccca acggattcgc attcgccacc
    13861 cgcttttcca ccgccctccc gcatggcggc ctcctcgagc tggagctgtt caatattctc
    13921 ggccagcggc ttacggcgcg ggcgttcaac ctgccgccgg gctggcatgc cttcgccctg
    13981 gagggcagcg ccctggccaa cggggtctat ttcctgcgcg ctgccagtgg gactcagcag
    14041 caggtgatca aaatggtcaa gatcggcccg tccgttcagg gaaacctgca gattctctac
    14101 cagggcactg gcgggcgccc ggccggcatg gccctggcca aaacggcgga ggcggatctc
    14161 taccgcttca ctgcctttgc cgcaggttat gtcgatgcgg tggtcgaaca aagg
//
LOCUS       DAOPWU010000050        14189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4375_length_14189_cov_5.522711, whole genome shotgun sequence.
ACCESSION   DAOPWU010000050 DAOPWU010000000
VERSION     DAOPWU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4375_length_14189_cov_5.522711"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PKM23_04360"
     CDS             complement(<1..356)
                     /locus_tag="PKM23_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY90737.1"
                     /translation="MKEIPIRSLLAALTPGRTLNLLKALAGGACGRISDRPLAWGAPY
                     VLSVEPTNLCDLHCPHCETGAGTLSRPTGYLDMELYRTILELNHGHLLYLLLYDQGEP
                     LLHPHFPEMVRLAKKQG"
     gene            complement(353..1174)
                     /locus_tag="PKM23_04365"
     CDS             complement(353..1174)
                     /locus_tag="PKM23_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY90738.1"
                     /translation="MWRGDLLENTLNSHERLWSNYDRWALERGRLAARLLAEWLPLPG
                     SRILDAGCGSGGASRALAEAGARVTAVDLFPTPPHGLAKMDAQIQYVCADLSAWSCDC
                     PNDGVILWDVLEHVPDPASALVNIRTALKPGGLALIATPNRWSPFNALCDPHYGLPGV
                     SLFNRKAVRRIVSGWLGWHRPDKADFPQLLSLGALENLLRKAGFSWRFINHSLFAAAM
                     DNPAGLWNSPWHLRLFTAIRAHGWENILDKALNDGAGLRNRLLMPTFFILARVET"
     gene            complement(1141..2235)
                     /locus_tag="PKM23_04370"
     CDS             complement(1141..2235)
                     /locus_tag="PKM23_04370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90739.1"
                     /translation="MIPLASIVFASYALILLWLAAGLIRLPRPHLASSPPPFLSVIVA
                     ARDEAVHLETCLEALSRQEYPGEWELFIIDDQSVDRSPDIAREAAARHPGWHTAGNDF
                     VGRWRSSKKGALETAFLRARGELLLFTDADCTPPPQWLAGMAAAMGSESVLCAGFSPL
                     VAPDASRLWRAFLLVDTLAAALVAAGGIGHGRGITCSGRSLACRRHILSEIGGYDALP
                     DTLSGDDDFLLQAVASSGRHRLIYALAPSTAVPAQGPSGWRAFIRQKRRHLSAGSRYK
                     LPIQLGYTLYHSANLLLWSTPLVGLIHRPLLLFLPAKIILDGLVLFLWSSRLHQPFSL
                     AGLLAWEPLFPVYHLVAFPRCGEEISWKTR"
     gene            2390..3091
                     /locus_tag="PKM23_04375"
     CDS             2390..3091
                     /locus_tag="PKM23_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNY90740.1"
                     /translation="MANKRDKLSDYLRKALWILSALALIIIVVAGIKYAYNRRYIRKI
                     AELEQVIKGMHSTMSVESVRQYNIQRIIAIVSQYNRTMPTHLKYEIAQEIHDACTNYT
                     SLDPDLLCAFITRETNGTWNPEYISDYGAMGLMQITPTIGSFIARSEHLNWISPDEVL
                     LNPVYNIRIGARYLSALIDAYDVDGALVARSLGERRAAMWVRSGRSNALLPRDTITYI
                     ADMLKQYDLMKGFKI"
     gene            complement(3088..4890)
                     /locus_tag="PKM23_04380"
     CDS             complement(3088..4890)
                     /locus_tag="PKM23_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNY90741.1"
                     /translation="MFFSCFLGKRGDDTQWQAALDDHARWLGLAVHRESCPCGEGWLF
                     AAAWLMPAAAPGFLPIRKREEQWSCSTDTGLADEAALDAEIGRAEKPGAFNYPDNPII
                     TFLPENRTLRITVPPTAPEQCFVAVDHRGLMMGNDLRLLRRWKPGALDPAAVYALLQF
                     RAIPPPLSLYTGIARIPNGHQFLGHAGEASFTFTPFFFPAQAGTREELTLISEEEALH
                     RFQQHLDENLHGLPDSSLLFFSGGVDSTLLAARLTALGKKKVQLVNFTFGPEDPEGKL
                     ALEMAALLGMPIEQLAYHEEEFPGMLTRIGREFSFPFGDSSAIPVNLMIHATLPRVGS
                     ARTVIEGVGPDQTFGAKLQDKKWGWAEAAPALVRGAAAAVYKGMALWKKESPPGTMAN
                     ILRKRWQMPREQAQIMAINALNGIAYATPEPLRRELDQAIHLHYHRLGEGLDADDLSC
                     LINLLHFSIGTVCAKSTDLYRLYGLRPVNPFMAREVLELSFALPHHIRCAQGKPKSLL
                     KKLAARSVPPALIYRPKQGFDPPLARMARHPVMLEYLHSLVLNDANPLLAYLHKPVMK
                     KMVGWIEGGRRLSYDAYRFIWMVLFASLWLDQQD"
     gene            complement(4935..6428)
                     /locus_tag="PKM23_04385"
     CDS             complement(4935..6428)
                     /locus_tag="PKM23_04385"
                     /inference="COORDINATES: protein motif:HMM:NF007773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="HNY90742.1"
                     /translation="MSIKQKAIHGVIWTGIAKASMQIVLFLLMLVLARLLSPEDFGIV
                     GMAAIVTVAISMVNDRGLGTAIIQKSTLSQEHLSSVFWGGIAFSLLLFAATIVASYPM
                     ALFFKNPLVQPVVAVQGAGFVIGSLGIVQKSILNKDLEFKKLAVMEVISSVASGVVSI
                     TLALMRFGVWSLVIGALVRDGVNVLLVWIYSRWRPRLFFSWRQFKELFSFSANVLGND
                     VVSYAVTNMDVVIIGRMLGTEALGYYTLALNLVKMPVTRLSGVVSKVAFPAFSAIQDD
                     MKKLRKGFLKASALLSLIIFPLLLGLALFAREFIALFMGEKWMPMAMPLIILVPMGLA
                     KSVGVIRWPVLMALGRPDVELKWNLAYILPLAACIYLGARHSLEGAAAGISLLLVLTF
                     PLVQTISDRIIHLGDREFYRSIAPAAAAGGAMVLVTLLAKSLLLPLISSNTLFLFIGG
                     FLIAAAVYMLVLLTTARPTLRNIREFIRPDGSSEKERRKKAASETNP"
     gene            complement(6530..7399)
                     /locus_tag="PKM23_04390"
     CDS             complement(6530..7399)
                     /locus_tag="PKM23_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90743.1"
                     /translation="MPAATPKSLLFIFAAALSVGFSCTENPFSGDDKINRVNRTISGQ
                     VRLNDSSSPAETWVWLETLNLGTRSAADGTFKLMLPSASAQPGGGLTGEFTLYFYRAN
                     YRLATRKIFLRTGTIEPDTGGLDSKGRIIEEILLTKLITLGTRVVPTSIPATADTSLF
                     VYLDLQGLVSSTSCGLPTIVMNQAVLLNGFLLQSEARPEIVHTVVPYPEHSRPLNYIP
                     APLEEVSYGCDYAVRAGMLAAGRYTVIPYVLIRQSALPEELLGSLGPAPESFDARYLV
                     LPFTRSCATLVVE"
     gene            complement(7411..9612)
                     /locus_tag="PKM23_04395"
     CDS             complement(7411..9612)
                     /locus_tag="PKM23_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY90744.1"
                     /translation="MIILIGLSVHSAWCQPVILKGRVLDVNTRNEIPFANIFVKGSQI
                     GTTSDFAGRFTLSVPEPSDETILVFQHVAYLPTEVKLGETWKNTILLQPRVIPIASVD
                     IQATSGGLQLSRDLPQAVSVFTAASLQLRGYMDAGDFLRTDHSVQVEEQLDGKKTVAI
                     RGGNADEVVVLYNGIRLNSAVDNIFDLSLIDLEDVDRMEVVKGSHTSLFGSDALAGAI
                     NIVSKVKQDYHLRLQQRFGTYNSGQWGVQAYLPAGRLHTAYRFKQGALRRQYEEGGLS
                     EEMENRSTHHTADLLFELPSSAGVPNLLTGTLIHSDSRFENPGEREKLADLNQVAALR
                     FEGGISVLRHLQGMVAWHHLKQEQEYVVRTAPARRLYHDRSLTASLGHTTNLGFSELL
                     LNYQFENTDVELGEYRGYQQNALLDYLSARFDRQHHGAVLIAKSRLASGSPTFPAAHI
                     DLSLRYDRLKEGEPRNLTAAGSAGALIYTPKTWQETTAKLSANLAGKGSDWLFNGYLN
                     YGSSVKFPTLLQQISAPYFASTVNPLEPERNRTSEIGLEFTRDLRDVPSLYGWRFEAN
                     IFRTYYDNKLRPYYSRGYTFAFYESLPDAQITGYEGKMTAFLARKKITTALGLARYDI
                     PEKAAFPFKSENKVTLDLRIDHTGFAFNAFIFRESDQVGWSRLGVNEFAEITLPGRTD
                     LDLHLSKNIPFHSFELLSSLSARNLLAADDAELSGLALRDRRFYLTLGLQY"
     gene            complement(9670..11127)
                     /locus_tag="PKM23_04400"
     CDS             complement(9670..11127)
                     /locus_tag="PKM23_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY90745.1"
                     /translation="MSKFPLRSKLTLIHLLLITVVTLFGSLFAIVTAQRYYQKRLDHF
                     IQTQLAQVQSLIEKSDLGDLSLPAHYAILRDYAHAAGFRLTLVDSSGGVRFDSNVGAD
                     SLLRMENHRDRPEIRQALRDGMGRQRRLSTTLNQPFYYGALRLKLDYLPQTAGETARY
                     IRIAYPLAEIEQQFSDLRTKILFANLMALLLIGLLSYWLAGRLSYPIQKLAAVAEKVK
                     RGDLEASFQHKSRDEIGELADLLNQMLGKLRVDLVQLRKLEQVRSQFLGNVSHELRTP
                     IFTLQGYLETLLNSKFDTPEQQKEFINRAYQQAGRLNNLLTDLIDISRIESGEMKLIF
                     RPFDVKALLARVVEELQSKAHYYGVTLTFASPASDEMLAANGDPERLTQAITNLAENA
                     IKYNLQGGKVEIGCRRIGEQIEIHVADTGRGIPQEHLPRIFERFYRVDKERSRAVGGT
                     GLGLAIVKHIVEAHGSRIEVQSEIDKGSRFSFRLK"
     gene            complement(11131..11823)
                     /locus_tag="PKM23_04405"
     CDS             complement(11131..11823)
                     /locus_tag="PKM23_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNY90746.1"
                     /translation="MAKKILIVDDEQEILDLLTFNLRREGYETLAVADGEAALVQAHA
                     ARPDLIILDVMLPGKDGWEVLRSLRQDPATRSVPVVFLTARESEIDEVVGLELGADDY
                     IIKPISMHKLLARIKLALRKANTPVESSTEILTFGAVAIDAANYSITIADQPVPFTKK
                     EFEILLYLVRRPGRVISRETLLNELWGDEVVVIDRTIDVHIRKIREKLGDTEMHHIET
                     IKGVGYRFITGT"
     gene            complement(11900..12634)
                     /gene="phoU"
                     /locus_tag="PKM23_04410"
     CDS             complement(11900..12634)
                     /gene="phoU"
                     /locus_tag="PKM23_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNY90747.1"
                     /translation="MKNHFSQEMASLKKNIQQVAELVDTQLEFAIRAMQTGNMELCKF
                     VRSRDREIDAYDNLIQAQCENILALFQPVAGDLRFIMAALMINNQLERCGDIAVNIVQ
                     RVQKTVDNRKLLEEIGLQQLGEEARGMARDAIEAFINNNLELAEAIRARDDQVDDHCR
                     AIFDTLIARMQAQSEWVEPGAHFIVLSRHLERLADHATNIAEEVIFLIEARIVAHQGD
                     QKGRLPLNKPPRSNRAEESAAAGNNP"
     gene            complement(12654..13412)
                     /gene="pstB"
                     /locus_tag="PKM23_04415"
     CDS             complement(12654..13412)
                     /gene="pstB"
                     /locus_tag="PKM23_04415"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNY90748.1"
                     /translation="MSTPAISAENFSFFYGSFKALNGINLTIEKNQVTALIGPSGCGK
                     STFLRSLNRMNEIIPNTRVEGRITLEGEDIYDRSVDVVDLRRRVGMVFQKSNPFPKSI
                     FDNVAYGLRINGIRDKALIEARVEQSLKDAALWDEVKERLGNSAMDLSGGQQQRLCIA
                     RALAVQPEVVLMDEPASALDPLATQKIEELVFSLKEHYTIVIVTHNMQQAARVSDVTA
                     FFYLGQLIEADRTERIFTTPSKKQTEDYITGRFG"
     gene            complement(13446..>14189)
                     /gene="pstA"
                     /locus_tag="PKM23_04420"
     CDS             complement(13446..>14189)
                     /gene="pstA"
                     /locus_tag="PKM23_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNY90749.1"
                     /translation="SWEFITAKPRKGMTEGGIWPAIVGTFWVSIVTILFSVPVGIFAA
                     IYLNEYASQGRLVRLVRLAIRNLSGVPSIVYGLFGFALFVNMANLGLSVLSAGLTLGL
                     LTLPWTITASEEALKTVPLSYREGALALGATRWQTIRTNVLPYAIPGMLTGTILGLAR
                     AAGETAPILFTGVAFFWPHLHGFPQVLTSQFMALPYHLFIMATQHHEIVKVRPLAYGT
                     ALVLLVLVLAMNFSATWIRYRFRKKFKRA"
BASE COUNT         2823 a         3772 c         4862 g         2732 t
ORIGIN      
        1 ccctgctttt tagccagccg taccatctcc gggaagtgcg gatggaggag aggctcgccc
       61 tggtcgtaga ggagcagata gaggagatgg ccgtgattga gttcaagaat ggtgcgatag
      121 agctccatgt ccaaatagcc ggtgggcctg gacagcgtcc cggctccggt ctcgcagtgg
      181 gggcagtgga ggtcgcagag attggtgggc tccacgctga gcacataggg agcgccccat
      241 gccagggggc ggtcggagat gcggccgcag gcgccgccgg cgagggcttt gagcaggttg
      301 agggtgcgtc ccggggtgag ggcagcgagg agggatctga tcgggatctc tttcatgttt
      361 ccaccctggc caggatgaaa aaggtcggca tcagcaagcg gttgcgcaaa cccgcgccat
      421 cgttgagcgc cttgtccagg atgttctccc aaccgtgagc tcggatcgca gtgaagaggc
      481 ggaggtgcca gggactgttc catagccctg cgggattatc catggccgcg gcgaagaggc
      541 tgtggttgat gaaccgccat gaaaatccgg ccttgcgcag gaggttctca agggcgccga
      601 gggagagaag ttggggaaaa tcggccttgt ccggacggtg ccagcccagc cagccggaga
      661 cgatgcggcg gaccgccttg cggttgaaca aggagactcc cggcaatcca tagtggggat
      721 cgcagagggc gttgaagggc gaccagcggt tgggagtggc gatgagggcg aggccgccgg
      781 gtttgagggc tgtgcggata ttcacaaggg ccgacgcggg gtccggtacg tgttcgagca
      841 catcccaaag gatgacgccg tcgttgggac aatcgcacga ccaggcggag aggtccgcac
      901 agacgtactg gatctgcgca tccattttgg ccaggccatg cgggggagtg gggaaaagat
      961 cgacggcggt gacccgggcg ccggcttcgg ccagggcccg gctggcgcct ccgctgccgc
     1021 agccggcgtc gaggatcctg ctgcccggca ggggcagcca ttccgccaaa agtcgggcgg
     1081 cgaggcggcc gcgctcgagc gcccagcggt catagttgct ccacaggcgc tcatgactgt
     1141 tcagcgtgtt ttccaggaga tctcctctcc acaccggggg aatgcaacaa ggtggtaaac
     1201 ggggaagagc ggctcccagg cgagcaagcc cgcgaggctg aagggctggt gcagtctgct
     1261 gctccacaaa aagaggacaa gtccgtcgag gataattttg gccgggagga aaagcaggag
     1321 cggccggtgg atgaggccga cgaggggggt tgaccagaga agcagattgg cgctgtgata
     1381 gagggtatag cccagttgga tcgggagttt atagcggctg ccggcggaaa ggtggcggcg
     1441 tttttgcctg atgaaggcgc gccatccgga ggggccctgc gcagggacgg cggtggaggg
     1501 ggcgagggcg tagatgaggc ggtgccttcc cgaggaggcg acagcctgga ggaggaaatc
     1561 gtcatcaccg gagagggtat cgggcagggc gtcatagccg ccgatctccg acaggatgtg
     1621 cctgcggcag gcgaggctgc ggccgctgca ggtgatgccg cggccgtggc caatcccccc
     1681 ggcagcgacc agggcggcgg cgagggtgtc cacgaggaga aaggcgcgcc aaagccgcga
     1741 tgcgtccggg gccaccagcg gagaaaagcc ggcgcagagg acgctttcgg accccatggc
     1801 ggcagccatg cctgccagcc actgcggcgg cggggtgcaa tcggcgtcgg tgaaaagtaa
     1861 aagctcgccg cgggcgcgga ggaaggccgt ctccagggcc ccctttttac tgctgcgcca
     1921 gcggccgaca aaatcgtttc cggctgtgtg ccagcctggg tgccgggcgg cggcctcccg
     1981 cgcaatatcc gggctgcggt cgacggactg atcatcgatg atgaatagct cccactcgcc
     2041 agggtactcc tgccggctca gggcttcgag gcaggtctcg aggtgtacgg cctcgtcccg
     2101 cgctgccacg atgacgctga gaaagggggg cggagaggag gccaggtgcg ggcggggcag
     2161 acggatcagg cccgcggcga gccagaggag gatcaacgca taagatgcga agactattga
     2221 ggctagtggg atcatcgcgg gcaaattatc cataattggg gaaacaagca agggaatttc
     2281 ccggcgacgc ggtgtgccgg tcacaaacgg aagatgatga aaagcgttga ttgatgcgca
     2341 agttttgctt attttagata aaccaaaccc tcttcgggat ggagcagcga tggccaataa
     2401 acgggacaaa ctatccgact atctcaggaa agccctgtgg atattgtcgg ctctcgccct
     2461 gatcatcata gtcgttgcgg gcatcaaata cgcctacaac cggcgctata tccgcaagat
     2521 cgccgaactc gagcaggtga tcaagggcat gcactcgacg atgagcgtcg aaagcgtgcg
     2581 ccagtacaac atccagcgca tcatcgccat cgtcagccag tacaaccgca ccatgcccac
     2641 ccatctcaaa tacgagatcg cacaggagat ccacgacgcc tgcaccaatt acaccagtct
     2701 ggaccccgat ctgctctgcg ccttcatcac ccgggagacc aacggcacct ggaatcctga
     2761 gtacatctcg gattacggcg ccatgggcct gatgcagatc accccgacga tcggatcttt
     2821 catcgcccgt tcggagcatc tgaactggat ctcaccggat gaggtgctgc tcaacccggt
     2881 ctacaacatt cgcatcggcg cccgttatct ctccgcactg atcgacgcct acgatgtgga
     2941 cggcgccctg gtggcgcgca gcctcgggga gcgccgggcc gcgatgtggg tgaggagcgg
     3001 ccggagcaat gccctgctgc cgcgcgacac gatcacctat atcgccgaca tgttgaagca
     3061 gtacgatctg atgaagggat tcaagatcta gtcctgctga tcgagccaga gcgaggcgaa
     3121 aagaaccatc cagatgaacc ggtaggcgtc ataactcagg cgcctaccac cctctatcca
     3181 tcccaccatt ttcttcatca ccggtttgtg cagataggcg agcaggggat tggcgtcgtt
     3241 cagaacgagg ctgtggagat actccagcat gaccggatgc ctggccatgc gggcgagagg
     3301 cggatcgaat ccctgtttgg gacggtagat cagcgccgga ggcacgctgc gcgccgccag
     3361 ttttttaagc agggatttgg gttttccctg cgcgcagcgg atatggtgcg gcagggcaaa
     3421 gctcagctcg agcacctccc tggccatgaa ggggttgacc ggccgcagcc cgtacaggcg
     3481 gtagaggtcg gtagacttgg cgcagacggt gccgatggag aaatggagca gattgatcag
     3541 gcaggagagg tcatcggcat caagtccttc accgagtcga tggtaatgga gatggatggc
     3601 ctggtcgagt tccctgcgca gcggctcagg agtggcatag gcgatgccat tgagggcatt
     3661 gatggccatg atctgggcct gctcgcgcgg catttgccag cgtttgcgca gaatattggc
     3721 catggttcca ggcgggctct cctttttcca cagcgccata cccttgtaga cggccgctgc
     3781 ggcccctctc acgagcgcgg gtgcggcttc cgcccatccc cattttttgt cctgcaactt
     3841 ggccccgaag gtttgatcgg gccccacccc ctcgatgacc gtgcgtgcgg agcccactcg
     3901 gggcagggtg gcatggatca tcaaattgac ggggatggcg gaggagtcgc cgaaggggaa
     3961 ggagaactcg cggccgatac gggtcagcat gccgggaaat tcttcctcat ggtaggcgag
     4021 ctgttcaatg ggcatgccga gcagcgcagc catctcaagc gccagcttcc cctcggggtc
     4081 ttcgggcccg aaagtgaaat tgaccagctg cacctttttc tttccgagcg cggtgaggcg
     4141 ggcggcgagg agggtcgagt cgaccccgcc gctgaaaaag aggagcgagg agtcgggcag
     4201 gccgtgcagg ttttcgtcca gatgctgctg aaaccggtgc agggcctcct cctcgctgat
     4261 aagcgtcaac tcctcccgcg ttccggcctg agccggaaag aaaaaggggg taaacgtgaa
     4321 gctggcttca ccggcgtggc cgaggaactg atggccgttg ggaatgcggg cgatgccggt
     4381 gtagagggaa agaggcgggg ggatggcgcg gaattgcaaa agggcataga cagcggcggg
     4441 atcgagtgcg cccggtttcc agcgccgcag caggcggagg tcattcccca tcatcaggcc
     4501 gcggtgatcg acagcgacaa agcactgttc gggggcagtc gggggcaccg tgatgcgcaa
     4561 ggtcctgttt tccggcagga aggtgatgat gggattgtcc ggatagttaa aggccccggg
     4621 cttctccgcc cgtccgatct cggcatccag agcggcctca tcggccaggc cggtatcggt
     4681 cgaacagctc cactgctcct cccttttcct gatcggcagg aatccgggcg cggcggccgg
     4741 catcagccag gccgcggcga agagccaccc ctcgccgcag gggcagctct cccgatggac
     4801 ggcgaggccg agccagcggg catggtcatc cagagcggct tgccactgcg tatcatctcc
     4861 cctcttcccc aaaaaacagc tgaaaaacat ggtcaggctc cgggtgtggt aaaaagatag
     4921 ttccgtcgtc cctgctaggg attggtttca gaagccgcct ttttacggcg ctctttttcg
     4981 ctgctgccat ctggtcggat aaactccctg atgttccgca gggtggggcg cgcggtggtg
     5041 agaagcacca gcatatagac cgccgcggcg atgaggaatc cgccgatgaa gagaaagagg
     5101 gtattggagg agatcagcgg cagcaagagt gatttggcca gcagggtcac caggaccatg
     5161 gcgccaccgg ccgctgcagc cggggcgatg gagcgataaa actcgcggtc tcccagatgg
     5221 atgatacggt ccgagatggt ttgcaccagg ggaaaggtga ggaccaggag cagggaaatg
     5281 cccgctgccg ctccttcaag gctgtgacgc gcgcctaaat agatgcaggc ggcgaggggc
     5341 aggatatagg cgaggttcca tttcaattca acatccggcc gaccgagggc catcagcacc
     5401 ggccagcgga tgacccctac cgatttggcc agtcccatcg gcaccaggat aatcagaggc
     5461 atggccatgg gcatccattt ctcccccatg aagagggcga tgaactcacg tgcgaagaga
     5521 gccagcccga gcagcagggg gaagatgatc agggagagca aggccgaggc tttgagaaag
     5581 cccttgcgca gctttttcat atcatcctgg atggctgaga aggccggaaa agcgaccttg
     5641 gagaccaccc ccgagaggcg ggtgaccggc atcttgacca ggttgagggc gagggtgtaa
     5701 tagccgaggg cctcggtgcc cagcatgcgg ccgatgataa ccacgtccat gttggtgacg
     5761 gcataggaga cgacatcgtt gccgaggacg ttggcgctga aggagaagag ctccttgaac
     5821 tggcgccagg agaagaagag gcgcgggcgc cagcgggagt agatccagac cagcaggacg
     5881 ttgacgccgt cgcgcaccag ggcgccgatg accaggctcc agactccgaa ccgcatcagg
     5941 gccagggtga tggagaccac tccgctggcc acggaggaga taacctccat gacggccagt
     6001 tttttgaatt cgagatcctt gttgaggatt gatttctgga cgatgccaag ggaaccgatg
     6061 acaaatccgg cgccctgaac cgcaaccacc ggctggacca ggggattctt gaaaaagagg
     6121 gccatgggat acgacgccac aatagtcgcc gcgaacagca ggaggctgaa ggcgatcccg
     6181 ccccaaaaga ccgaggagag atgttcctgg ctgagggtgc ttttctggat gatcgcggtg
     6241 ccgagcccgc ggtcgttaac catggagatc gccacggtga cgatggcggc catgccgaca
     6301 atgccaaaat cctccggaga gagcaggcgc gccagaacca gcatgaggag gaagaggacg
     6361 atctgcatgc tcgccttggc gatgccggtc cagattacac catggatagc cttttgtttg
     6421 atggacatac gattccttga gaataggccc tgcgcagcgg ggcccggatg gagacaggtt
     6481 atacatagtg tagccgcgcg cccttcactt tgcggatagc gatgggcggc tattcgacga
     6541 cgagtgtagc gcagctgcgt gtgaagggca ggaccagata gcgtgcatca aaggattcag
     6601 gggcggggcc gaggctaccc aagagttcct cagggagagc gctctgccgg atgaggacgt
     6661 aaggaatcac tgtatagcgg cctgcggcca gcatgccagc cctgacggca tagtcgcagc
     6721 cgtaggagac ctcctccagc ggagcgggga tgtaattgag gggccggctg tgctcaggat
     6781 agggcacgac cgtgtggacg atttcaggcc tggcttcgct ttgcagaaga aaaccgttca
     6841 gcagcacggc ctggttcatg acgatggtgg gtaggccgca cgaggtggag ctgacaaggc
     6901 cctggaggtc cagatagacg aagagcgagg tgtcagcggt ggcggggatg gaggtgggca
     6961 caacgcgggt gccaagggtg atcagcttgg tcagcaggat ctcctcgatg attctgccct
     7021 tgctgtccag gccgccggtg tcgggctcga tggtgccggt tcgcagaaag attttacggg
     7081 ttgccaggcg gtaattggcg cggtagaaat agagagtaaa ctctccggtc aggcccccgc
     7141 ccggctgggc ggaggcggag gggagcatga gtttgaaggt gccatccgcc gcgctgcggg
     7201 tgccgaggtt gagggtttcg agccagaccc aggtttcagc gggtgagctg ctgtcgttaa
     7261 gccgcacctg gccgcttatg gtacggttga cccggttgat tttgtcatcg ccggagaagg
     7321 ggttttcggt gcaggagaag ccgacagaca gagcggcggc aaagataaaa agcagggatt
     7381 tcggggtggc ggctggcatg ataaatatcc ttaatattgc aggcccaggg tcagatagaa
     7441 gcggcggtcg cgcagggcca gtcccgagag ttctgcgtca tcggcggcga gcagattgcg
     7501 ggcgctcagg ctgctgagga gctcgaagct gtggaacggt atattcttgc tgagatgcag
     7561 gtccagatcg gtgcgtccgg gcagggtgat ttcagcgaat tcgtttaccc ccagccggct
     7621 ccagcccacc tgatcactct cacggaagat gaaggcattg aaggcgaagc cggtatggtc
     7681 gatacgcaga tcgagggtga ccttgttctc cgatttgaag ggaaaggccg ctttttcagg
     7741 aatgtcatag cgggccaggc cgagagccgt ggtgattttt ttcctggcga ggaaggcggt
     7801 catcttgcct tcgtagccgg tgatttgggc atccggaagg gactcataaa aggcgaaggt
     7861 gtagccgcgg ctgtagtagg gtctgagttt gttgtcgtaa taggttctga agatattggc
     7921 ctcgaagcgc cagccgtaga gagaggggac gtcacggagg tcgcgggtga actcgaggcc
     7981 gatttcggag gtgcgatttc tttcgggctc gaggggattg acggtggagg cgaagtaagg
     8041 ggcgctgatc tgctgcagca gggtcgggaa tttgacgctg ctgccgtagt tgagatagcc
     8101 attgaaaagc cagtcgctgc ctttgccggc gagattggcg gataatttgg cggtggtctc
     8161 ctgccaggtt ttcggggtgt agatcagggc tccggcgctc ccggcggcgg tgagattccg
     8221 gggttcgccc tccttgagcc ggtcatagcg caggctgagg tcgatgtggg cggcggggaa
     8281 ggtgggggag ccgctcgcca gccggctctt ggcgatgagc acggcgccat gatgctggcg
     8341 gtcgaagcgg gctgaaagat agtccagcag tgcattctgc tggtagccgc ggtattcgcc
     8401 cagctccaca tcggtgttct caaactggta gttgaggagc agctcgctga agcccaggtt
     8461 ggtggtgtgg ccgaggctgg cggtcaggct gcgatcgtgg tagaggcggc gggccggcgc
     8521 cgtgcgcacg acatattcct gttcctgttt gaggtgatgc caggcgacca tgccctggag
     8581 atgacgcaaa acggagatgc cgccttcgaa ccgcaaggcc gcgacctggt tgagatcggc
     8641 gagcttttcc ctctccccgg gattttcgaa acgggagtcg gaatggatca gtgtgccggt
     8701 gagaaggttc ggcactcctg cgctggaagg cagttcaaag aggaggtcgg cggtatggtg
     8761 ggtggagcgg ttctccatct cctcgctcaa gccgccctct tcatactgac gccgcagggc
     8821 cccctgcttg aagcggtagg ctgtgtgcag ccgcccggcc gggaggtagg cctgcacgcc
     8881 ccactgccct gagttgtagg tgccgaagcg ctgctgcagg cgtaaatggt agtcctgctt
     8941 gaccttgctg acgatgttga tggcgccggc gagggcatcc gagccgaaga gggaggtgtg
     9001 gcttcccttg acgacctcca tgcggtcgac atcctcgagg tcgatgaggg agaggtcaaa
     9061 aatgttgtcg acggcactgt tgagccggat gccattgtaa aggaccacca cttcatcagc
     9121 gttgccgccg cggatggcga cggtcttttt gccgtcgagc tgttcctcga cctgcacgct
     9181 gtggtcggtg cgcaaaaaat cgccggcatc catatagccg cgcagctgaa gtgaggcggc
     9241 agtgaatacc gagacggcct ggggcagatc tctggagagc tgcagaccac ccgaagtggc
     9301 ctggatgtcg acggaggcga tgggaatcac ccggggctgc aggaggatgg tgtttttcca
     9361 ggtttcgccc agttttactt cggtggggag gtaggcgaca tgctggaaaa cgaggatggt
     9421 ctcatctgag ggctcgggga cggaaagggt gaagcgtccg gcgaagtcgc tggtagtgcc
     9481 gatctgggag cctttcacaa agatgttggc aaaggggatc tcattgcggg tgttgacgtc
     9541 gagcacccgg cctttgagaa tcaccggctg gcaccaggcg gaatggacag agagtccgat
     9601 caagatgatc atatgaagag atttcatact gattccgtta aaagcagggt gacgggctgg
     9661 ccggatacgc tatttgagcc ggaagctgaa acgggagcct ttgtcgattt cgctttgcac
     9721 ctcgatgcgg ctgccgtggg cttcgacgat atgtttgacg atggccagtc ccagaccggt
     9781 accgccgacg gcgcgtgagc gttccttgtc cacccggtaa aagcgttcga atatgcgcgg
     9841 cagatgttcc tgggggatgc cgcggccggt atcggccaca tggatttcaa tttgctcgcc
     9901 aatgcggcgg cagccgatct ctaccttgcc gccctgcagg ttatacttga tggcgttttc
     9961 ggccagattg gtgattgcct gggtcagccg ttcgggatcg ccattggcgg ccagcatttc
    10021 gtcggaggcg gggctggcga aggtgagcgt caccccgtag tagtgggcct tgctttgcag
    10081 ctcctcgacg actctggcga ggagggcttt cacatcaaag gggcggaaga tcagcttcat
    10141 ttcgccggat tcaatgcggc tgatatcgat cagatcggtg aggaggttgt tgagtcttcc
    10201 cgcctgctga taggcccggt tgatgaactc cttttgctgt tccggggtgt cgaatttgga
    10261 gttgagcagg gtttcgagat agccctgcag ggtaaaaatc ggggtgcgca gctcatgtga
    10321 gacattgccg aggaactggc tgcgcacctg ttcgagcttg cgcagctgca ccaggtcgac
    10381 gcgaagtttg ccgagcatct ggttgagcag gtcagccagt tcgccgatct cgtcgcggct
    10441 tttgtgctgg aagctggcct ccagatctcc gcgtttgacc ttttcggcga cggcggcgag
    10501 cttctggatg ggataggaga gccggccggc cagccagtag ctgagcaatc cgatcagaag
    10561 cagggccata aggttggcga agaggatctt tgtgcgcaga tcgctgaact gctgttcgat
    10621 ctcggcgagg ggataggcga tgcggatata gcgggcggtt tcgcccgcgg tttggggcag
    10681 atagtcgagt ttgagacgga gggcgccata gtagaagggc tgattcaggg tggtgctgag
    10741 gcgtctctga cggcccatgc cgtcgcgcag ggcctggcgg atttccggcc ggtcgcgatg
    10801 gttttccatg cgcaagagcg aatcggcgcc aacgttggag tcgaagcgaa ccccgcccga
    10861 ggaatcgacc agagtcagcc ggaatcctgc cgcatgggca taatctctca ggatggcata
    10921 gtgggccggc agggagaggt cgcccaaatc gctcttttcg atcaggcttt gcacctgggc
    10981 gagttgggtc tgaataaagt ggtccaggcg cttctgataa taacgctgag ccgtcacaat
    11041 ggcaaagagg ctgccgaaga gggtaaccac cgtgatgagc aggaggtgga tcagagtgag
    11101 cttgcttctg agaggaaatt ttgacatgat tcaggtcccg gtaatgaatc ggtagccgac
    11161 gcctttaatg gtttcgatat gatgcatctc agtgtcgccg agtttttcac ggatcttgcg
    11221 gatgtggacg tcgatggtgc ggtcgatgac cacgacctca tcgccccaga gttcgttgag
    11281 cagggtttct ctgctgatga cgcgtccggg tcgccgcacc agatagagga ggatttcgaa
    11341 ctctttcttg gtaaagggga ccggttgatc cgcgatggtg atgctatagt tggcggcgtc
    11401 gatggcgacg gcgccgaagg tcagtatttc cgtcgatgac tcgaccggcg tgttggcctt
    11461 gcgcagggcc agcttgattc gcgccagcag cttgtgcatg ctgatgggtt tgatgatata
    11521 gtcatccgcg cccagctcaa ggccgaccac ctcgtcgatt tcgctctccc gggcggtaag
    11581 aaacaccacc ggcacgctgc gggtggctgg atcctggcga aggctgcgca gcacctccca
    11641 gccatccttg ccgggcagca tcacatcgag gatgatgagg tcgggtctgg ctgcatgtgc
    11701 ctggacgagg gcggcctcgc catcagcgac ggccagggtt tcatagccct cccttcggag
    11761 attgaaggtg agcagatcga ggatctcctg ctcgtcatcg acgatgagaa ttttctttgc
    11821 cataaagatc ccgtctgggc ataagcggcc ggtcgctgcg gcgcccgttt atttaagata
    11881 atcctttatt cggcgattct caaggattat ttcccgcggc cgccgattct tcggcgcgat
    11941 tgctccgggg cggcttgttc agggggagcc ggcccttctg atcgccctgg tgggcgacga
    12001 tgcgcgcctc gatcaggaag atgacctctt cggcgatatt ggtggcgtgg tcagccaacc
    12061 gttcgaggtg gcgggagagg acgataaaat gggcaccggg ttcgacccat tccgactggg
    12121 cctgcatcct ggcgatgagg gtgtcgaaga tagcgcggca gtggtcatcc acctggtcat
    12181 cccgcgcgcg gatcgcttca gccagctcga gattgttgtt gataaaggct tcgatggcat
    12241 cgcgggccat cccgcgcgcc tcctcgccca gctgctgcag cccgatctcc tcgagcaact
    12301 tgcgattgtc gacggtcttt tgcacacgct gcacgatatt gacggcgatg tcgccgcacc
    12361 gttcgagctg attgttgatc atcagggcag ccatgatgaa gcggaggtcg ccggcgaccg
    12421 gctgaaagag ggcgaggatg ttctcacatt gcgcctgaat gagattgtca taggcgtcga
    12481 tctcgcgatc gcggctgcgc acaaatttac acagctccat gttgccggtc tgcatggcgc
    12541 ggatggcgaa ctcgagctgt gtgtcgacga gttcggccac ctgctggatg ttctttttca
    12601 ggctcgccat ttcctggctg aagtgatttt tcatctgagg ctccttgggg ggatcatccg
    12661 aagcggccgg tgatatagtc ttcggtctgc tttttggatg gtgtggtgaa tatcctttcc
    12721 gtgcggtccg cttcgatgag ctggccgaga taaaaaaagg cggtgacatc actcacccgg
    12781 gccgcctgct gcatgttgtg ggtgacgatg acgatggtat agtgctcctt gagcgagaaa
    12841 accagctcct cgatcttctg agtcgccagg gggtcgagag cggaggccgg ctcatccatc
    12901 agcacaacct ccggctgaac cgccagggcg cgggcgatgc agagccgctg ctgctgtccg
    12961 ccggagagat ccatggcgga gttgccgagg cgctccttga cctcatccca gagggcggcg
    13021 tctttcaggc tttgctctac gcgcgcctcg atgagggcct tgtccctgat gccgttgatg
    13081 cgcaggccgt aggcgacgtt gtcgaagatc gatttgggaa aggggttcga tttctggaag
    13141 accataccga cacgacggcg caaatcgacc acatcgaccg agcggtcata gatgtcctcg
    13201 ccctcgagag tgatgcgtcc ctccacccgg gtgttcggga tgatttcgtt catgcggttg
    13261 agcgagcgca gaaaggtcga cttgccgcat ccggaggggc caatcagggc ggtgacctga
    13321 tttttctcga tggtcaggtt gatgccgttc agggccttga atgagccgta gaagaaggaa
    13381 aaattttccg ctgagatcgc cggggttgac atgggctttt ccttatctga atcgatgccg
    13441 cgcggtcagg cgcgtttgaa ttttttacgg aagcggtagc ggatccaggt ggcgctgaaa
    13501 ttcatcgcca gcacgagcac cagcaggacc agggcggtgc cataagccaa cggccgcacc
    13561 ttgacgatct cgtggtgctg ggtggccatg atgaaaagat gatagggcag ggccatgaac
    13621 tggctggtca gcacctgggg aaagccgtgg agatgaggcc agaaaaaggc cactccggtg
    13681 aagaggatgg gggcggtctc gccggcggcg cgcgccaggc cgagaatcgt cccggtcagc
    13741 atgccgggga tggcataggg cagcacattg gtgcggatgg tctgccatct ggtggcgccc
    13801 agggccagcg ccccttcccg gtaggagagg ggtacggtct tgagcgcctc ctcgctggcg
    13861 gtgatggtcc agggcagggt caacagcccg agggtgaggc cggcggagag gactgagagg
    13921 ccgaggtttg ccatattcac aaaaagtgcg aatccgaaga gtccatacac gatggagggt
    13981 acgccggaga ggttgcgaat ggccaggcgg accaggcgca ccagtcgtcc ctgcgaggcg
    14041 tactcgttga gatagatggc ggcgaagatg ccgaccggca ctgaaaaaag gatggtgact
    14101 atgctcaccc agaaggtgcc gacgatagcc ggccagatgc ccccctcggt catgcccttg
    14161 cgcggcttgg cggtgatgaa ttcccagct
//
LOCUS       DAOPWU010000051        14028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4447_length_14028_cov_6.021542, whole genome shotgun sequence.
ACCESSION   DAOPWU010000051 DAOPWU010000000
VERSION     DAOPWU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14028
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4447_length_14028_cov_6.021542"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PKM23_04425"
     CDS             <1..560
                     /locus_tag="PKM23_04425"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513920.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HNY90750.1"
                     /translation="LGRRLAEVPVGFKWFVEGLLDGSCGFGGEESAGASFLRRDGRVW
                     TTDKDGIILNLLAAEITAVTGRDPGEHYGALEERFGRPVYQRLDAPADAAQRAALANL
                     SPEMVRSDTLAGEAIRDKLTRAPGNSAAIGGLKVVTENGWFAARPSGTEDIYKIYAES
                     FLGADHLARIQEEARAMVTAALQAG"
     gene            complement(616..1806)
                     /locus_tag="PKM23_04430"
     CDS             complement(616..1806)
                     /locus_tag="PKM23_04430"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNY90751.1"
                     /translation="MKDIRWAGLLLLLVWAGAAAGQTAPARKGGVNMTRLALIDKAVG
                     QAMDRGYIPGAVVLVNHGGDKVWHKAYGFAQLVPEKVAMEQEMMFDLASVTKPVATAT
                     SIMILAERGLLRLTDQVSDYVPGFSRYVRGDTTLADEALIWHLLTHTSGLPAYINAAE
                     AARVFGSSTKTETMVDYISKLPKLSGPGERYLYSCLGFITLNQIIIRVTGQNVSEFAQ
                     ENIFRPLEMRYTTFNPDETLKLICVPTELQEDGLPLRGIVHDPLARLQGGISGNAGLF
                     ATASDLARYCQMMLNGGILGETRILSPVTVSRMTQIIDTPAKAARGYGWVIKLGQSWV
                     GGDLLPDGGYGHTGYTGTSIWIDPRTQTFIIILTNRVHPKDDGEVDSLRSTIANIVAS
                     AILE"
     gene            2072..3736
                     /locus_tag="PKM23_04435"
     CDS             2072..3736
                     /locus_tag="PKM23_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HNY90752.1"
                     /translation="MVREYLFILLGLALMLGCNGLNSRASSRFIDEHVARIEPISREA
                     NLAYWEAATTGKAAAYDRYAELELQKRQIYSNAEEYAFLKKMKEQGGLGDAVVAREIE
                     LLYLAYLPNQIEPELLREITTLGAEVEKKFSIFRGTAGKRRLSGNQIEEILKSEKKTG
                     LRREAWLASKQVGTEVAQELLRLVRLRNKAAQKLGFRNYHALSLAVTEQDEKELNKIF
                     EELYDLTNGPFFALKHELDEELARRFHTRIEELQPWHYQDPFFQESPMLESVNLDQYY
                     AGRDIRLLAADFYRGIGLPIDSILAKSDLYEREGKNPHAFCTDIDRRGDVRILCNLTD
                     SERWMETILHEGGHAVYSYYTNPALPYLLRQEAHAFTTEAIANFFGRLSRNPWWMEEM
                     LGLTPEQRQELLQVSARYARLKQLIFSRWAMVMYNFEKELYADPEQDLNALWWTLVEK
                     YQGIKKPAGRDEPDWAAKIHFTIVPCYYHNYLLGEMLASQLHACLTRSVLREGGQAGY
                     VGATEAGAWLRERVFGAGASWSWNEMIKRATGEPLTARYFVEQFVN"
     gene            3744..5075
                     /locus_tag="PKM23_04440"
     CDS             3744..5075
                     /locus_tag="PKM23_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012731452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HNY90753.1"
                     /translation="MNNHNGAMIRTMPDGTEKQYNPFTGTEVWHIPGRKNRPIFTGIP
                     ESAQKLVDVNMQDVCNFCPSRYLITPPEKSRVVVRDGQWVTQHQLSPVDVVKEVAEFR
                     RIPNLFEIVSINYWKKNFQYHLSEKNRAWKESYLAVPEGRQHVLDIIDLKLRYSGLEE
                     KEIKAIPMDQKLEMADAFFGGGHELIVPRRHYIDGAEYDSQLASSGELSAEEHYHYFR
                     FTIEAMNEVYADNRYCRYVAVYQNWRKEAGASFDHLHTQIVGLDEWGVSLERELKLMA
                     TNRNMYNEYAANFAALNNNVICENDYAMAFVDLGHRYPTIAIYSKSVNCRPREHTPEE
                     LRGVSDLVHAVHAATGSQISGNEEWYYMPNDSIVPIPWHILIKWRVNIPAGFEGGTKI
                     YVNPMSLSELRDKLVPRLFELRYQGRLGSLKIAEECPVKPNSLKYYHRSLE"
     gene            5084..5689
                     /locus_tag="PKM23_04445"
     CDS             5084..5689
                     /locus_tag="PKM23_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90754.1"
                     /translation="MGVAWTKRAGGWLLLAVVLAVGCKTPFSTREPENPVTKQSTWVQ
                     PTSPALVMVNLRTAMAERNSSNYLRCLADTSLAPASFSYQPESAVGAANPGLFLRWDK
                     ESERNFLNQLFAFLPSDSVSSLSLTLIRENTFQDSVILVQEYVLTVAHRRQQEAPRQM
                     RGQAEFRLLRSSEELWYIRGWADYATGTNPTWSALRAYFGK"
     gene            5741..6922
                     /locus_tag="PKM23_04450"
     CDS             5741..6922
                     /locus_tag="PKM23_04450"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HNY90755.1"
                     /translation="MKKEQLKRLQWALGTLAVLMLAIHGYILLKVPSSGVEVIPEQGQ
                     REPEARKEASAPEAARKESSPVEAPADPRDRASQKEVEQVGEALRKALASFRLTAPTV
                     RKSGEAYTVSIPHNLNIHRLQFELMEQLDEVDGKIVKAIEDRRRDRMEYTVEVDGDWA
                     CRIVLMQRESISALSGRMAIIVDDFGYANNELVRKFLYYPKPLTIAILPGQKASGVVA
                     RDARVANREILVHMPMEPLNERFSDEGYTLLAGQDAGTARLRVRAALAQIPHAIGINN
                     HQGSRVTADRVLMKAVMTELKHQDKLFVDSRTSSQSVALQVARELGLRAGANQVFLDA
                     EDKESFIEGQFEKAAAIAGKQGEVIAICHMRKRTFKVLERMIPRLEQQGIRFVYLSEV
                     L"
     gene            7012..7731
                     /locus_tag="PKM23_04455"
     CDS             7012..7731
                     /locus_tag="PKM23_04455"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNY90756.1"
                     /translation="MPRLCLCVNQVAQLRNINRKKEPDPLQVAMAAELAGIDGIVVQL
                     REDRSDISDRDLRLLKEVVKSHLNLAIPMQEEMVKKALQLLPDMVTLLPVLQPGEGDE
                     HSMNVEGNLDYFEEIAAALRANNIVVSALVAADPAQIRAAARAGADYVQFNTTAFSSV
                     EDLGTLTEQLERLRAVASAANKLGLGVAAGRGLGYQHIRDIAGIPLIEEVNVGRSILA
                     RSMLVGVERAVTTMKNQFEHK"
     gene            7741..8274
                     /locus_tag="PKM23_04460"
     CDS             7741..8274
                     /locus_tag="PKM23_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNY90757.1"
                     /translation="MEKKLLSLFPEFGLIQDVGLRDRTLACWMEAMKLGGWQVEDLDT
                     IPFTLLIPDCRVSFRTHVQAVTQTAIASAKILSGHYAPYYTLSMDLIVSGGLLHDIGK
                     LLEYRRVEGKFVKSSNGKLLRHPFSGANLAARHLLPDEIVHIIAVHAKEGDGGYRTPE
                     AVVVHHADFMNFEPLKG"
     gene            8343..9716
                     /locus_tag="PKM23_04465"
     CDS             8343..9716
                     /locus_tag="PKM23_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNY90758.1"
                     /translation="MEFPAEPFRVKVVEPVRRTTREERDRLIREAGYNVFALPAASVY
                     VDLLTDSGTSAMSDYQWAGMMMGDESYAGSKNYYHFEETIRSIFGFRYIIPTHQGRAA
                     ENLLFSVALREGDLVPNNIHFDTTRANVLHKRGVPLDLVIDEAMFPEKEHPFKGNMDP
                     AKLEKAFQVHGPGKIPLVMLTVTNNSAGGQPVSMENVRQVSAIAKKYHTPFIIDACRY
                     AENCYFIKEREPGYGGRSLTEIARELFSYADGCTMSAKKDALVNIGGFLALNDEGWMD
                     KAKNLLILLEGYPTYGGLAGRDLEAVARGLEEGLDEDYLAYRVGQVRALGERLDRAGV
                     PFVKPTGGHALFLNAGAILPQIPRENFPAQALVVALYREAGIRAVEIGGLMFGEKEAD
                     GSEKYPALELVRLAIPRRVYTSLHLTYVADAVIEIFRNREKIKGLRLVHEAALLRHFT
                     AQLEEVG"
     gene            9713..10366
                     /locus_tag="PKM23_04470"
     CDS             9713..10366
                     /locus_tag="PKM23_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNY90759.1"
                     /translation="MIGALLFDFDGVISDSIEIHFRGWQEVLPPLGIEPDNLVLRSHE
                     GEPAWRIAQEMCAAAGRPIDEKEAHRLAGLKNSWFRGQPRPELYPGVSEILDYAGRVG
                     IRTAVVTGTNRQNLHHVLGEMSTRFDALFCDGDYARPKPHPDPYLAAVAHFGLPPEAC
                     TVVENAPMGIRAARSAGLFCIALQTTLPAAHLQEADLILPDHTALLRWLEESAPEIA"
     gene            10588..11736
                     /locus_tag="PKM23_04475"
     CDS             10588..11736
                     /locus_tag="PKM23_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90760.1"
                     /translation="MRRLILLSLPLLILAGCGKRELPTPPAPEEVVLSQAMLPEMLGP
                     MRLYRIEIGAAGEVDSVLLEAVPRGTGAAEQRFYLYDDGGARHAGDGDVVAFDNIFSQ
                     QILWQPTLPGFREYELRFQASRAGRTVGEPLVLMRSSGDQRAPEVAQLLLPDTLRSGF
                     SGSLLFQAVVIDSSGPGDVARVELTGSAAGKASFDTLLYDDGSHGDPAAGDYIYSLAV
                     DRTFGARKQGAYTIRIAAVDRSGQRSPEKSAILVIVNTAPLLTDLNAPAAVQRPAAGA
                     SSHLVSVRIDEPQGLQDINRVLLRAYNPDGTGFNNNPFVMYDNGLALDISRWDLGYRG
                     DLTAGDGVYSMTVIFDAAKPLGVYRITFTAEDWAGNQSELLTHTITLD"
     gene            11758..13182
                     /locus_tag="PKM23_04480"
     CDS             11758..13182
                     /locus_tag="PKM23_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90761.1"
                     /translation="MKRILLLALLAPAALGAAVITTHRFSTAAPKSGLAGNGITDIAR
                     SGGHLWLGTGNGLSRLDLATGQFSSFAPTQGLGHGSVSALWAHGDTVIVATATDTVTS
                     ADPAPLPKGTGISFSFDSGATWRRSPQPGPTPIQNLTYDIAVFAGTVWITSYGGGVLK
                     SPDWGASWVEAAPDTFNFDPYGKYNHRGFSAAAGGGALWIGTAEGINKSTDNGATWRN
                     LTHVSQPEPISGNFVVALGCQHAGGRDIVWGATWVAEGETEYNAVSRSLDGGLTWKSF
                     LRDEKAHNFAFDDSVVYVAADKGLFVSYDLGENWYLYPPMRDARDGDRVLGTEAYSAY
                     ALAGTLWAGMADGLARTSDNGFTWDIYRAFTPTGRGGEKRTYAYPNPFSPMRQNQIGG
                     DGYVRFQYNTRTAARVTVKVFDFAMELVATVIDGEYRIGPSECAEIWNGRNDYGDLVA
                     NGAYFYSIEIENDGTYWGKVLIIN"
     gene            13206..>14028
                     /locus_tag="PKM23_04485"
     CDS             13206..>14028
                     /locus_tag="PKM23_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90762.1"
                     /translation="MKRFGAIAGLLLMLALGASASEGDGGYAGAFLRMGIGARAKAMG
                     DAFSAVAEDAFAGVYNPALLPHLPGRLAALSYAFLPLDRKLDFIGYAQPLRPGNGEDE
                     EHNPLRAGFGLAWIHAGVDGIDGRDLAGNHTQDYSFSENAFFLSFALSPAEKFSIGVN
                     GKILYARMPGVNNDESAVTSMGLGVDFGAYYAPFRQLSLGLVMRDNLSKYTWNTEKVW
                     ERGTSVNDRFPRTLRLAVAYRPPVEWLLLTAESEDAKEQNPRYHFGAEAALPAVGA"
BASE COUNT         2694 a         4285 c         4392 g         2657 t
ORIGIN      
        1 cgctcggccg ccggctcgcc gaagtgccgg tcggctttaa atggtttgtc gaggggctgc
       61 tggacggctc ctgcggtttt ggcggcgagg agagcgcggg ggcttcgttt ctgcgccggg
      121 atggccgggt ctggacgacc gacaaggacg ggatcatcct caatctgctc gccgcggaga
      181 tcacggcggt gaccggcagg gatccgggcg agcattatgg cgccctcgag gagcgattcg
      241 gccggccggt ctatcaacgt ctcgatgccc cggcggacgc agcgcagcgg gccgccctgg
      301 cgaatctctc tccggagatg gtgcgttctg acaccctggc aggcgaggcg atccgggaca
      361 agctcacccg ggcgccgggg aacagcgccg ccatcggcgg cctcaaggtg gtgacggaga
      421 acggctggtt cgcggcgcgc ccctccggca ctgaggatat atacaagata tacgcagaga
      481 gttttctcgg cgccgaccac ctcgcccgca tccaggagga ggccagggcg atggtgacgg
      541 cggccctgca ggccgggtga gatccctgcc ggcagcgcct ggcagcagcg ttgtgggacc
      601 ctcgccggcc tgcgactact cgaggattgc gctggcgacg atgttggcga tggtgctgcg
      661 caggctgtcg acctcaccgt catccttggg gtggacccgg ttggtgagga tgatgataaa
      721 ggtttgggtc ctgggatcga tccagatcga ggtcccggta taaccggtgt ggccgtagcc
      781 gccgtcgggg agcaggtcgc cgccgaccca actctgcccc agtttgatga cccaaccgta
      841 gccgcgtgcg gccttggcag gtgtatcgat gatctgggtc atgcgcgaga ccgtgaccgg
      901 gctgaggatg cgggtctcgc cgaggatgcc accgttgagc atcatctggc agtagcgcgc
      961 caggtcgctg gcggtggcaa agagtccggc attgccagag attcccccct gcaggcgtgc
     1021 gagaggatcg tgcacgatcc cgcgcagggg caggccgtcc tcctggagct ccgtcggcac
     1081 acagatgagc ttgagggttt cgtcgggatt gaaggtggta tagcgcatct ccaggggtct
     1141 gaagatgttc tcctgggcaa attcactgac gttctgaccg gttacacgga tgatgatctg
     1201 gttgagggtg atgaaaccca gacagctgta aaggtagcgc tctcccggac cgctcagttt
     1261 gggcaatttc gagatatagt cgaccatcgt ttccgtcttg gtcgaggagc caaagacccg
     1321 ggcggcttca gcggcattga tgtaggcggg cagacccgag gtgtgggtga gcagatgcca
     1381 gataagcgcc tcatcggcga gggtggtgtc tccccgcaca tagcgtgaaa agccgggcac
     1441 gtaatcggag acctggtcgg tgaggcggag caggccgcgt tcggcgagga tcatgatcga
     1501 ggtggcggtg gcgaccggct tggtcaccga agcgaggtcg aacatcatct cctgctccat
     1561 cgccaccttt tcgggcacca gctgggcaaa gccgtaggcc ttgtgccaca ccttgtcgcc
     1621 cccgtggttg accagaacca ccgcccccgg aatatagccc cggtccatgg cctgtccgac
     1681 cgccttgtcg atcagggcca gccgggtcat gttcacccca cccttgcgcg ccggtgccgt
     1741 ctgacctgcg gccgcgccgg cccagaccag aagcagcagc agacccgccc agcgaatatc
     1801 cttcataaag cctcccgcat atgtgcgcca attgagctct cttcagcgta gaataaaaaa
     1861 aatgaacctc atttgcaagc aaaaagcgca gcggcggcca tgcgcctttg cgtgcagccg
     1921 accctatctg ccaccgccag ggaggcccgg ccttacccgg cgcaggagaa gaggtgaaaa
     1981 ttgccttgga aatcggcgat aattttgcta ttattaaaat tgaccgcagc atattgatgg
     2041 ttgttaactc tatagataaa catggagttc gatggtgaga gaatatttat tcatcctgct
     2101 cggcttggcc ctgatgctgg gctgcaacgg tctcaattcg cgggcctcga gccgcttcat
     2161 cgatgaacat gtcgccagga tcgagcccat ctccagagag gccaacctcg cctactggga
     2221 ggccgccacg accggcaagg ccgcggccta tgaccgctac gccgagctcg aactgcagaa
     2281 gcggcagatc tacagcaatg ctgaagagta tgctttttta aaaaagatga aggagcaggg
     2341 cggactgggc gatgcggtgg tagcccgcga gatcgagctc ctctatctgg cctatctgcc
     2401 caaccagatc gagccggaac tgctgcggga gatcaccacc ctgggcgccg aggtcgaaaa
     2461 aaagttcagc atcttccggg gcaccgcggg caagcggcgg ctgagcggca accagatcga
     2521 agagatactg aagtcggaaa aaaagacagg cctgcggcgt gaggcctggc tggcgagcaa
     2581 gcaggtcggg accgaggtag cccaggagct gctccgcctg gtgcgtctgc gcaacaaggc
     2641 cgcacagaag ctgggcttcc gcaactatca cgccctttcc ctggccgtga ccgagcagga
     2701 tgagaaggag ctgaacaaga tctttgagga gctctacgat ctcaccaacg gccccttttt
     2761 cgccctcaag cacgagcttg atgaagagct cgcccggcgt ttccacaccc gtatcgaaga
     2821 gctgcagccc tggcattatc aggacccctt tttccaggag tcgccgatgc tggagtcggt
     2881 taatctcgac cagtactatg ccggccggga tatccggctg ttggcggccg atttctaccg
     2941 gggcatcggc ctgccgatcg attcaatcct ggccaaaagc gatctctacg agcgcgaggg
     3001 taaaaatccg catgccttct gtaccgatat cgaccgccgc ggcgatgttc gcatcctctg
     3061 caatctcacc gacagtgagc gctggatgga gaccatcctg cacgaggggg ggcatgccgt
     3121 ctacagctat tacacgaatc ctgcgctgcc ctatttactg cgccaggagg cgcatgcctt
     3181 caccaccgag gcgatcgcca actttttcgg gcgcctgagc cgcaacccct ggtggatgga
     3241 ggagatgctc ggcctgacgc cggagcagcg ccaggagctg ctccaggtca gtgctcggta
     3301 cgcccggctc aagcagctga ttttttcgcg ctgggcgatg gtgatgtata actttgaaaa
     3361 agagctctat gccgatcccg agcaggatct gaacgcgctc tggtggaccc tggtcgagaa
     3421 ataccagggc attaaaaagc cggccggcag ggatgaaccc gactgggcgg cgaagatcca
     3481 tttcacgatc gtcccctgct actatcacaa ttacctcctc ggagagatgc tggcttcaca
     3541 gcttcacgcc tgcctgacga ggtcggtact cagggagggg gggcaggcgg gttacgtcgg
     3601 tgcaacggag gccggcgcct ggctgcgtga aagggtcttc ggagccgggg cctcctggtc
     3661 gtggaacgag atgatcaaac gcgccactgg cgagccgctg acggccaggt atttcgtcga
     3721 gcaatttgtc aactgaggtc gagatgaaca accacaacgg tgcgatgatc cgcacgatgc
     3781 cggacggaac ggagaagcag tacaatccct tcaccggcac ggaggtgtgg catattccgg
     3841 ggcgaaagaa ccggccgatc ttcaccggca tcccggagag cgcgcaaaaa cttgtcgacg
     3901 tcaatatgca ggatgtctgc aatttctgcc cctcgcgcta tctgattact cccccggaaa
     3961 agtcgcgagt cgtggtccgg gacggtcaat gggtgacgca gcatcagttg agcccggtgg
     4021 atgtggtcaa ggaggtcgcc gaattccgcc gcatccccaa cctcttcgaa atcgtctcga
     4081 tcaattactg gaagaagaat ttccaatacc acctctccga aaagaatcgc gcctggaagg
     4141 agagttatct tgccgtgcct gaagggcggc agcacgttct cgatatcatc gatctgaagc
     4201 tgagatactc cggcctcgag gagaaggaga tcaaggcgat acccatggat cagaagctgg
     4261 agatggccga cgcctttttc ggcggcggcc atgagctgat tgtgccgcgg cgccactaca
     4321 tcgacggggc cgagtacgac tcgcagctcg cctcctccgg cgagctctcg gctgaggagc
     4381 attatcacta tttccggttc acaatcgagg cgatgaacga ggtttatgcc gacaaccgct
     4441 actgccgcta tgtcgcggtc tatcagaact ggcgcaagga ggcaggggcc tctttcgacc
     4501 atctgcacac ccagattgtc ggacttgacg agtggggggt ttccctggag cgcgagctca
     4561 agctgatggc gaccaaccgc aacatgtaca acgagtatgc cgcaaatttc gccgccctca
     4621 acaacaacgt catctgtgaa aacgattatg cgatggcctt tgtcgatctc ggtcatcgtt
     4681 atccgaccat cgccatctat tccaagagcg tcaactgccg gccgcgggag cacacccccg
     4741 aagagctccg cggcgtcagt gatctggttc atgccgtgca cgccgccacc ggttcccaga
     4801 tctccggcaa cgaggagtgg tactacatgc ccaacgacag catcgtgccc atcccgtggc
     4861 acatcctgat caagtggcgc gtcaacattc ccgccggttt cgaggggggg accaagatct
     4921 atgtcaatcc gatgtcgctg agcgagctgc gcgacaagct ggtgccgcga cttttcgagc
     4981 tgcgctatca gggcaggctg ggttcactca agattgccga agagtgcccg gtcaaaccca
     5041 attcgctcaa gtattatcat cgttccctgg agtaaccgtt tctgtgggcg tagcgtggac
     5101 aaagagggcg gggggctggc tgctgctggc ggtggtgctg gcggtgggct gcaagacccc
     5161 attcagcacg cgcgagccgg aaaatcccgt caccaagcag tcgacctggg tgcagccgac
     5221 ctctccggcc ctggtgatgg tcaatctgcg cacggccatg gccgaacgca acagcagcaa
     5281 ctacctgcgc tgtctggcgg ataccagcct ggcgccggcc tccttctcct accagcccga
     5341 gtcggcggtc ggcgcagcca atcccgggct ctttctgcgc tgggacaagg agagcgagcg
     5401 caattttctc aatcagctct tcgcctttct gccctcggac tcggtcagtt cgctcagttt
     5461 gaccctgatc cgggaaaata cctttcagga ctcggtgata ctggttcagg agtatgtgct
     5521 gacggtggcg caccgccggc agcaggaggc accgcggcag atgcggggtc aggcggaatt
     5581 ccggctgctg cgttccagcg aggagctctg gtatatccgc ggctgggccg actatgccac
     5641 cggcactaat ccgacctgga gcgccctgcg cgcctatttt ggcaaatgag tgagaaaccg
     5701 gagctgtgga gagtgaccaa tcggggaagc cgccgatagc atgaaaaaag aacaactcaa
     5761 acggttgcag tgggcactgg ggacgctggc cgtgctgatg ctggccattc atggctatat
     5821 tctgctgaag gtcccttcca gcggggtgga ggtcatcccc gagcaggggc agcgcgagcc
     5881 ggaggccagg aaagaggcgt cggcgcccga ggcggcgcga aaagagagtt cgccggtcga
     5941 ggccccggcc gatccgcgcg accgggcctc gcagaaagag gtggagcaag tgggggaggc
     6001 cctgcgcaag gccctcgcct ctttccgtct cactgccccg accgtgcgca agagcggtga
     6061 ggcctatacg gtctcgatcc cgcacaatct caatattcac cggctgcagt tcgagctcat
     6121 ggagcagctt gacgaggtcg acggcaaaat cgtcaaagcg atcgaggacc gccgcaggga
     6181 ccgcatggag tacaccgtgg aggtcgacgg cgactgggcc tgccgcatcg ttctgatgca
     6241 gcgcgagtcc atctcggccc tctccggccg catggcgatc attgtggatg atttcggata
     6301 tgcgaacaac gagctggtgc gcaagtttct ctattacccc aagccgctga cgatcgccat
     6361 cctgcctggg cagaaggcaa gcggggtggt ggcgcgcgac gccagggtgg cgaaccgcga
     6421 gattctcgtc cacatgccca tggagccgct caacgagaga ttttccgatg agggttatac
     6481 cttgctcgcg ggtcaggatg cgggcacggc gcggctgcgg gtgcgcgcag cgctggcgca
     6541 gatcccccat gccatcggca tcaacaatca ccagggctcc agggtgacgg ccgaccgcgt
     6601 actcatgaag gcggtgatga ccgagctgaa acatcaggac aagctctttg tcgacagccg
     6661 cacctcatcg caaagcgtgg ctctgcaggt ggcgcgcgaa ctcgggctgc gcgcaggggc
     6721 caaccaggtt tttcttgatg ccgaggacaa ggagagcttt atcgaggggc agttcgaaaa
     6781 ggcggcggcg attgcaggta agcagggaga ggtcattgcc atctgccaca tgcgcaagcg
     6841 gaccttcaag gtgctggagc ggatgattcc ccggcttgaa cagcagggga tccgcttcgt
     6901 ctatctttcg gaagtgctgt aaccgggcgc cgcagcaggg cgcagcctgt tcatccagtt
     6961 cagcgcagga gcgcacatcg gccctcctgc tcacctaatg tgaggtctcc catgccaaga
     7021 ttatgccttt gtgtcaacca ggttgctcag ctgcgcaata tcaaccgcaa gaaagagccg
     7081 gatcccctcc aggtggccat ggccgccgag ttggcgggca tcgacgggat tgtggtgcag
     7141 ctgagggagg atcgctcaga catcagtgac cgtgatcttc ggctcctgaa ggaggtggtc
     7201 aagagtcatc tcaacctggc gatccccatg caggaagaga tggttaagaa ggccctgcag
     7261 ctgctgccgg acatggtcac ccttctaccc gtgctgcagc cgggggaggg ggacgaacat
     7321 tcgatgaatg ttgagggcaa tctcgactat ttcgaagaga tcgcggcagc cctgcgcgcc
     7381 aataacattg tggtcagtgc actggtggca gccgatccgg cgcagatccg cgccgcagcg
     7441 cgggcggggg ccgattatgt gcagttcaac accaccgctt tctcttcggt ggaggacctc
     7501 ggcaccctga ccgaacagct cgaacggctg cgcgccgtgg cctctgcggc gaacaagctc
     7561 ggcctgggcg tggccgccgg ccgcggcctg ggctaccagc atatacgcga catcgccggc
     7621 atccccctca tcgaggaggt caatgtcggc cgctccatcc tcgcccgcag catgctggtc
     7681 ggggtcgagc gcgccgtcac taccatgaaa aatcaattcg aacacaaata ggcggacaga
     7741 atggaaaaaa aactcctctc cctttttccg gaattcggtc ttatccagga tgtcggactg
     7801 cgcgacagga ctctcgcctg ctggatggag gccatgaagc tcggcggctg gcaggtcgag
     7861 gatctcgaca ccatcccctt caccctcctc atcccggact gcagggtcag ctttcgcacc
     7921 catgttcagg cagtgaccca gacggccatc gcctcggcca agatcctgag cgggcattat
     7981 gccccctatt acaccctctc catggatctc atcgtcagcg gcggcctgct gcacgacatt
     8041 ggcaagctcc ttgagtaccg gcgtgtggag ggcaaatttg tcaagagcag caacggcaag
     8101 ctgctgcgcc atcccttcag cggcgccaat ctggccgcca gacatctgct gccggacgag
     8161 atcgtccaca tcattgcagt ccatgccaag gagggcgacg gcggctaccg cactccggag
     8221 gctgtcgtcg tacatcatgc cgactttatg aattttgagc ccctcaaggg ctgaacctgc
     8281 ccctcccagc cgtcggcttg tacaccggcc cggccgggga gaggaaatga atcggggtct
     8341 atatggaatt tcctgctgaa cccttccggg tcaaggtcgt cgaacccgta cgccggacca
     8401 cccgcgagga gcgcgaccgc ctcatccgtg aagcgggata taacgttttt gccctgcccg
     8461 ccgcgagcgt ttatgtcgat ctgctcaccg acagcggcac ttcggccatg agcgactatc
     8521 agtgggcggg gatgatgatg ggcgacgaat cctacgccgg cagcaaaaac tattaccact
     8581 ttgaggagac catccgctcc atcttcggct tccggtatat catccccacc catcaggggc
     8641 gtgccgccga gaatctcctt ttttccgtcg ccctcaggga gggcgatctg gtgcccaaca
     8701 acatccattt cgacaccacg cgtgccaacg tcctccacaa acggggtgtg cccctcgacc
     8761 tggtcattga cgaggcgatg ttcccggaaa aggagcatcc cttcaagggc aacatggatc
     8821 cggccaagct ggagaaggct tttcaggtgc atggcccagg caagattcca ctggtgatgc
     8881 tcaccgtgac caacaacagc gccggcggtc agccggtttc gatggagaat gtccgccagg
     8941 tgagcgccat cgctaaaaag taccacaccc ctttcatcat cgatgcctgc cgctacgccg
     9001 agaactgcta tttcatcaag gaacgcgagc cgggctatgg cggcaggagc ctcacggaga
     9061 tcgcccgcga actgttttcc tacgccgacg gctgcaccat gagtgccaag aaggatgccc
     9121 tggtcaacat cggaggattt ctcgccctca acgacgaggg gtggatggat aaggccaaga
     9181 atctgctcat cctgcttgag ggctatccga cctacggggg cctggccggg cgggatctcg
     9241 aggccgtcgc gcgcggactg gaggaggggc tcgacgagga ctatctcgcc taccgcgtcg
     9301 gccaggtgcg cgcactgggc gagaggctgg accgggcggg agtgcccttt gtcaaaccca
     9361 cgggtgggca cgcccttttt ctcaacgccg gtgcaatcct gccccaaatc ccgcgggaga
     9421 actttccggc ccaggctctg gtggtggccc tctaccgcga ggccggcatt cgcgccgtcg
     9481 agatcggcgg gctgatgttc ggcgaaaagg aggccgacgg cagcgaaaag tatcctgcgc
     9541 tcgagctggt ccgtctggcc ataccgcgtc gggtctacac ctcccttcac ctcacctatg
     9601 tggcggatgc ggtcatcgaa atcttccgca accgcgagaa gatcaagggg ctgcgtctgg
     9661 tgcacgaggc ggccctgctg cgtcatttca ccgcacagct cgaggaggtc ggttgatcgg
     9721 cgcgctgctc ttcgattttg acggggtgat ctccgattcg atcgagattc atttccgggg
     9781 ctggcaggag gtgctgcccc ccctcgggat cgagccggac aacctggtgc tgcgctctca
     9841 tgagggggag ccggcctggc gtatcgccca ggagatgtgc gcagcggccg gccggccaat
     9901 cgatgaaaag gaggcgcacc gcctcgccgg gctcaagaac agctggttcc gcgggcaacc
     9961 caggccggag ttataccccg gggtgagtga gatcctcgat tatgccgggc gcgtcgggat
    10021 caggacggcg gtggtgaccg ggacgaaccg gcagaacctg caccatgtgc tgggggagat
    10081 gagcacccgc tttgatgccc tcttttgcga cggggattat gcgcggccca aaccgcaccc
    10141 cgatccctat ctggcggccg tggctcattt cgggctgccg ccagaggcct gtaccgtggt
    10201 ggagaatgcg ccgatgggca tccgtgccgc caggagcgcc gggcttttct gcatcgctct
    10261 gcagacgacg cttccggccg ctcacctgca ggaggccgat ctcatcctgc ccgatcacac
    10321 cgcgctgctg cggtggctgg aggaatcggc cccagaaatt gcttgactaa tatgattttt
    10381 gtggctattt tatataagct tattcgattg aacaaggaga acgcctattt cccttcgtta
    10441 aagatatttt ctgtgtgaac ttgcatacaa tctctttgac gaatggtaga ggcgcctttt
    10501 taaacttgtc cgaatgtagc cgggtgaagc gtgcgtacca tcgtcgctcc ggtcattcgg
    10561 attttttttt aacctacaag gattatcatg cgtcgattga tcttgctctc tctccccctg
    10621 ctcatcctgg cgggctgcgg caagcgcgaa ctgcccactc cgcctgcacc tgaagaggtg
    10681 gtgctgagcc aggccatgct gcccgagatg ctcgggccaa tgcggctcta ccggatcgag
    10741 atcggtgctg cgggtgaggt ggattcggtc ctgctggagg cggtgccgcg tggaaccggc
    10801 gcggcggaac agcgtttcta tctctacgac gacgggggag cgcgtcatgc cggcgacggg
    10861 gatgttgtgg cctttgacaa catcttttcg cagcagatcc tctggcagcc aaccctcccc
    10921 ggtttccggg agtacgagct gcgttttcag gccagccgtg caggacgcac ggttggggag
    10981 ccgctggtcc tgatgcgcag ctcgggtgat cagcgcgcac cggaggtcgc gcagctcctc
    11041 ctccccgaca cccttcgcag cggtttttcc gggagtctgc tctttcaggc tgtggtcatc
    11101 gattccagcg gccccggcga cgtcgcccgc gtcgagctca ccggcagcgc cgcaggcaag
    11161 gcctccttcg acaccctgct ctatgacgac ggcagccatg gcgatccggc agccggcgac
    11221 tatatctata gcctggcggt ggaccgcacc ttcggcgccc gcaagcaggg ggcttatacg
    11281 atcaggattg cggcggtgga caggtcgggc cagcgcagcc ctgaaaagag cgccattttg
    11341 gtgatagtca acacagcccc gcttttgacg gacctcaacg ccccggcggc ggtacagcgg
    11401 ccggcggcgg gcgcttccag tcacctggta tcggtgcgga tcgatgaacc ccaggggctg
    11461 caggacatca accgggtgct tctgcgcgcc tataatccgg atggcaccgg ctttaacaac
    11521 aatccctttg tgatgtatga caatggtctg gcgctcgaca tcagccgctg ggatttgggc
    11581 tatcggggag acctgacggc cggtgacgga gtctattcca tgacggtcat ctttgacgcc
    11641 gccaagccgc tcggggtcta ccggatcacc ttcacggccg aggattgggc cggcaatcag
    11701 agcgaactcc tgacccatac gatcacccta gactgacgag atggttgcat tatgagaatg
    11761 aagagaattc tgctcctggc cctgctcgcg cccgcagccc tcggcgcggc cgtgatcacg
    11821 acccaccgtt ttagtacagc ggccccgaag agcggcctgg ccggcaatgg catcaccgat
    11881 atcgcccgca gcggcggaca tctctggctg gggaccggca atggcctgag ccggctggat
    11941 ctggccaccg gccagttcag cagctttgcc ccgacccagg gactgggcca tggcagcgtc
    12001 tccgcgctct gggcccatgg cgacaccgtc atcgtcgcca ctgcgaccga tacggtcacc
    12061 tccgccgatc ccgccccctt gcccaagggg acgggcatca gtttttcttt cgacagcggc
    12121 gccacctggc gccgttcccc gcagccgggt cccactccga tacagaatct cacctacgac
    12181 atcgccgttt tcgccggcac ggtctggatc acctcctatg ggggcggcgt gctcaaatcc
    12241 cctgactggg gggcgagctg ggtcgaggcg gcaccggata cctttaactt tgatccctac
    12301 ggcaagtaca atcaccgcgg cttttccgcc gccgccgggg gcggggcgct ctggatcggc
    12361 accgccgagg ggatcaacaa gagcacggac aacggcgcta cctggcggaa cctgacccac
    12421 gtcagtcagc cggagccgat ctcggggaac tttgtcgtcg ccctcggctg ccagcacgcc
    12481 ggcggccgcg acatcgtctg gggcgccacc tgggtcgccg agggcgagac cgagtacaat
    12541 gcggtctcgc gttcgctcga tggcggcctc acctggaaga gctttctgcg cgacgaaaag
    12601 gcccacaatt tcgcctttga cgattcggtc gtctatgtgg ctgcggacaa gggtctcttt
    12661 gtctcgtacg atctgggcga gaactggtat ctctatcccc cgatgcggga cgcccgcgac
    12721 ggtgaccgcg tcctcggcac cgaggcctat tccgcctacg ccctggcggg caccctgtgg
    12781 gcgggcatgg cggacggcct ggcgcgcacc tccgacaacg gcttcacctg ggacatctac
    12841 cgcgccttca cacccacagg ccggggcggc gagaaacgca cctatgccta tcccaatccc
    12901 ttctcgccga tgcgtcagaa ccagatcggg ggagacggct atgtccgttt ccagtacaac
    12961 acccggacgg ccgcgcgggt gacggtcaag gtcttcgatt tcgccatgga gctggtcgcc
    13021 acagtgatcg acggcgagta ccgcatcggc ccctccgagt gcgcggagat ctggaacgga
    13081 cgcaatgatt atggcgacct ggtcgccaac ggggcctatt tttacagtat tgagatcgag
    13141 aacgacggca cctattgggg caaggtgctg atcattaact agactggtca ggattgagga
    13201 aacttatgaa gagatttggt gcgatcgccg gcctgctgct gatgctggcg ctgggcgctt
    13261 cagcctcgga gggtgacggg ggctatgcgg gggcctttct gcgcatgggg atcggggcgc
    13321 gggccaaggc catgggcgac gccttttccg cagtggcgga ggatgccttc gccggggtct
    13381 acaatccggc cctgctgccg catctgcccg gccgcctggc ggcgctctcc tacgcctttt
    13441 tgccgctcga ccgcaagctc gatttcatcg gctatgccca gcccctccgg ccggggaacg
    13501 gcgaggatga ggagcacaat cccctgcgcg ccggttttgg cctggcgtgg atccatgccg
    13561 gcgtcgatgg gatcgacggc cgcgacctgg ctggcaacca tacccaggat tactccttct
    13621 ccgaaaacgc ttttttcctc tccttcgccc tgagtcccgc tgaaaaattt tccatcgggg
    13681 tcaacggcaa gatcctctac gcccggatgc cgggcgtcaa caacgacgaa tctgcggtca
    13741 cctcaatggg tctgggcgtc gatttcgggg cctattatgc gcccttccgg cagctcagcc
    13801 tggggctggt gatgcgcgac aacctctcca aatacacctg gaacaccgaa aaggtatggg
    13861 agcgcggcac cagcgtcaac gaccgttttc cgcgcacctt gcgactggcc gtagcctacc
    13921 gcccccccgt ggagtggctg ctgttgaccg ccgagagcga ggatgcgaaa gagcagaatc
    13981 cgcgctatca tttcggggcc gaggccgccc tgccagcggt cggggcgc
//
LOCUS       DAOPWU010000052        13910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4488_length_13910_cov_5.382317, whole genome shotgun sequence.
ACCESSION   DAOPWU010000052 DAOPWU010000000
VERSION     DAOPWU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13910
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4488_length_13910_cov_5.382317"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKM23_04490"
     CDS             <1..196
                     /locus_tag="PKM23_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNY90763.1"
                     /translation="TGIAGPGGATSEKPVGLVYVGYAGKGGAAVEEHRVISGDRLVNK
                     ERFAYIAMNLLRKQLFNLQT"
     gene            196..756
                     /gene="thpR"
                     /locus_tag="PKM23_04495"
     CDS             196..756
                     /gene="thpR"
                     /locus_tag="PKM23_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNY90764.1"
                     /translation="MATLRTFICIELSEKVRERIAELQQQLKPLGSGVSWTRPEGIHL
                     TLKFLGDIESARVADIAGAVERAAQGVAPFRITVAGTGGFPNLSRPRVLWVGIESSAS
                     ALFVVQSQIEIELARLGYPREERRFSPHLTLGRVKSPEAVREICRELLQRSFAPVTFT
                     AGSIIIMRSDLKPDGALYTPLRTIEL"
     gene            772..1815
                     /gene="recA"
                     /locus_tag="PKM23_04500"
     CDS             772..1815
                     /gene="recA"
                     /locus_tag="PKM23_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNY90765.1"
                     /translation="MPEERDEKRKALELAMTQIDRQYGKGSIMWLGEDKKIPIDVIST
                     GSISLDAALGVGGVPRGRIIEIFGPESSGKTTLALHVIAEAQKAGGLAAFVDAEHALD
                     AHYANLLGVDTENLLISQPDTGEQALEITETLVRSGALDVVVIDSVAALVPRAEIEGE
                     MGDAQMGLQARLMSQAMRKLTAAISKSKTCVVFINQIREKIGVMFGSPETTTGGRALK
                     FYSSVRLDIRRTASIKEGENVIGNRTKVKVVKNKVAPPFREAEFDIIYGQGISKIGDL
                     LDLAVNMNIVEKSGTWFSYKEERLGQGRENVRKYLDQNPELLATIEKGVRQNLGLFPG
                     AGGEEAGEKAEKK"
     gene            1843..2481
                     /locus_tag="PKM23_04505"
     CDS             1843..2481
                     /locus_tag="PKM23_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNY90766.1"
                     /translation="MAEMRTVTGIEMQKRDAQRISIFLDGEFAFGLHQDVLLESGIAR
                     GDRLSEERIEAILALEQKRRAKEKALRLLSVRSRSRKELADRLRQAKFSPEAIEYTLG
                     EMERLGFLNDGDFAQAWGRNRTATRPAGAFMLRQELRRKGVAEEDIEKGLQAAFRESS
                     EAEVARELAIRRKQTLAALPEEKARKRLQDFLLRRGFGWDLVSELMEEWERL"
     gene            2582..5224
                     /gene="alaS"
                     /locus_tag="PKM23_04510"
     CDS             2582..5224
                     /gene="alaS"
                     /locus_tag="PKM23_04510"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNY90767.1"
                     /translation="MTSQEIRQSFLDFFKSKNHHIVPSAPVVPQDDPTLLFTNAGMNQ
                     FKDVFLGLRQRQWARVADSQKCIRVSGKHNDLEEVGRDTYHHTFFEMLGNWSFGDYFK
                     AEAIEWAWELLTGVWHLPADKLYATVFAGDELDKVPGDEEAAEYWASRTAIDPAHILR
                     FGKKDNFWEMGESGPCGPCSEIHIDRGPGFCTNTDPGHVCAVNSGCARFIELWNLVFI
                     QYNRDEEGVLNTLPARHVDTGAGFERLVSVLQKTRSNYDIDLFVPIIAEISRVTGRPY
                     GEGEEGVAFRVLCDHIRALSFAIADGAIPGNEGRGYVLRRILRRAARFGRLLGMREPF
                     IYKLVKPLADSMGEAYPELRQRQSHIERVIRSEEESFGRTLDRGIEIFEEKAAASPGG
                     VLSGRDAFLLHDTYGFPLDLTQLMAAEKNLSVDVAGFEAEMEEQRQRSSRARDVSYEK
                     IEVDAVMGEGSQFRGYQVDILETELIHYEPGRIVLRESPFYAESGGQVGDRGEIGNDH
                     FRFAVETTKHVGEHIVHFGELREGSAPVPGERVEASIDAERRRATERNHTVTHLVHRA
                     LRTVLGEHVQQAGSLVAPDYMRFDFTHFEKMSAEQIRAVEDIVNREILQNRPVHWQVM
                     PIEEAKALGAMALFGEKYGDEVRMVEVQDFSRELCGGTHVRATGEIGLFVLRGESAIA
                     AGVRRLECLTGESARAWLQGRADIAAQSAVLLGCRDEEVVERLERLQQERKALEGEMR
                     KLRHSTSRDIVGELVRGAHKVAGISVAAAQIEAAGVDELKQTADLLREGLQSGVGVLA
                     AVIGDKVQFVCVVTKDLVEGRKLKAGDIVREIAAVAGGSGGGSPHMALAGARDAGKIA
                     EALNGVEKIIAEMITRLEPGATQG"
     gene            5256..5999
                     /locus_tag="PKM23_04515"
     CDS             5256..5999
                     /locus_tag="PKM23_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNY90768.1"
                     /translation="MKVYEQLLQIREEKGAGYLVLIDPDKQSMQEAIELARVCSESGV
                     DGLLLGGSLMFTHIFDDLIKGIKTASPLPLIIFPGSTRQLSPYANAILYLSLISGRNA
                     YYIIGDQVLAAPIIKSMNLEAISTAYMLIESGNITSAQFLSDTRPIPREKPDISVAHA
                     MAAELLGMRMIYLEAGSGAHLSVPEEHIAAICRYTSLPVIVGGGIRAPEEAARKVAAG
                     ASFIVTGNVIEKNRDRALISAFADSIHTR"
     gene            6026..6820
                     /locus_tag="PKM23_04520"
     CDS             6026..6820
                     /locus_tag="PKM23_04520"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90769.1"
                     /translation="MVLVDTHAHLLPFWDDGADRWDTALGMLRAAEEDEIGAVVCTPH
                     ILSPKDMEHEDALWVRYEELVGRSAEAGLKVKIHMGSELFAHPGIDLKRRIATPAQNG
                     RYFLVEFPMGSMPDFVLKGFFTQIGKEYTPVIAHPERYARIIQNPREAMILVEKGALL
                     QINAGSLLGVFGGAIKETAVKLLDANLVHMVASDAHDLKARPLRLQPAFTVVAEKYGR
                     EKAQRLFADNPARILRGEDVIADPPRPIEEAPKRGFWQRLEIMTKK"
     gene            6829..7413
                     /gene="gmhA"
                     /locus_tag="PKM23_04525"
     CDS             6829..7413
                     /gene="gmhA"
                     /locus_tag="PKM23_04525"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNY90770.1"
                     /translation="MERLTFIEEQMRESVELFEAIRREMGPAISRGAEMMIDTLRSGG
                     KLLICGNGGSAADAQHFAAELVGRLQRERKALPAIALTTDTSILTAVGNDYGFAKIFR
                     RQVEAWGQPGDLLLAISTSGNSPNVLEAIAAARAQGMQVLSLAGRSGGEMAGLSDLAL
                     VMPARSSQHIQEGHIAIIHIWCALVEESLCPPVS"
     gene            7432..9525
                     /locus_tag="PKM23_04530"
     CDS             7432..9525
                     /locus_tag="PKM23_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNY90771.1"
                     /translation="MNFLNSAILMGLAAVALPILIHLFTRARAKPIPFSSLRFLKQLR
                     NQKIRRIKIRQILLLLLRTLAVLFLVLGFARPTCRTQSGSGARSRTSAVLLLDNSASM
                     ALEEQGESLFAAAREAGARIIELMQPGDELYLCTTTDTLEGTERRAFHDFQAFRRRLE
                     ATPLSFRATDISAGMRFAQNLLQSAHNVNKELYLLSDMQATGFAADSLPAREFNPRQY
                     AVPLLASHPANLAVTGVRLRSAILERGKTVEVEVTLANSGQEPGRTKLVQLFIHGQRV
                     AQRMVSLERGTTAQELFRFIVDRDGWIEGYVQLEDDALMEDNLRYFTFYVPERLNVGV
                     IGERTAGSELLQLALQSSADSSAFLRLFTAVPERSSGLAIDSLQLLLLHDVSRLPDAV
                     VERIGAWHSRGGGIILVLGRRTDIGWYNARLAPVLGLSPVLAAFGEGGHFSLGRVDGT
                     HPVFSGIFEGAEIQFARPQFNFACRVASAPDQHALLSFSTGDPYLYEVRRDEGALLVF
                     TSSFEPEVSDMAYRTIFAPLLHRSISYLAAHGQNRGVELSAGEVLRYRLAGQELQKQL
                     EIHRPDGGVDLVHPGITASGAWIDHAATGIPGLYSLRADGKRLAVWAVNIPARELDLR
                     RAERELVEDSHHLHWVDDPARIDQFIREERIGREYWRELLLAGLLLLLLEMALYREKG
                     ELPSEEESQEMRRGS"
     gene            9522..10817
                     /gene="hflX"
                     /locus_tag="PKM23_04535"
     CDS             9522..10817
                     /gene="hflX"
                     /locus_tag="PKM23_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNY90772.1"
                     /translation="MKESQERERAVIVGLITRETPSWRVEDYLDELEQLADTAGAEVI
                     ERVVQERERRDPAFMIGRGKAEELARTARYQDADLIIFDDDLTPAQAKNIEKLCGVKI
                     VDRSALILDIFAKHARTREARTQVELAQLQYLLPRLTGQWRHLERQVGGIGVRGPGET
                     QLEVDRRLVRKRVAVLQKELEKIAGQREIRRKNRRDFFKAALVGYTNVGKSTLLNALT
                     EAGVLVEDRLFATLDATVRTLPMDNRRRLLLIDTVGFIRKLPHHLVASFKSTLEESAD
                     ADLLLHVIDISHPNFGEQMNVVDKVLKELNLGGRPVLKVFNKIDQFDQSGVVARLRET
                     EQPCVFVSAARGIGLPQLVERLAEFADAAFVTDELVLPLEKASRVPKLYEVVEVLNTS
                     YGEEEMAVQIRFARQEERRVRSQVAKILQMDEEDGSAHE"
     gene            10810..12486
                     /locus_tag="PKM23_04540"
     CDS             10810..12486
                     /locus_tag="PKM23_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90773.1"
                     /translation="MNRGASWLTAVLILLWVAGAAAQSEGRIRGTNRHYRHGDWTTWS
                     SMRHIRHLCLSQERLYLATTGGIGCFNFFSQQWEEPHTVSSGLASADIGLVAWDENSG
                     FLWCTQSEGISYLGPASGVWTNLFYDEIGFYDRESVSSIGFGVNHQIYLVTSRGRSLA
                     GHSSGGGFEWGATPRPEEEVKWFGEAAAVDPPPPHLFLPPGYYYDEQERVITDSHGRR
                     YPLTCWLRDAWNTLWIGTWGLGAARVDLVSARFEALPYGLWDDAVDVLAFDQGALWAG
                     GDQGQTGGGGLTRWEIGIRNPEYYEPRYITGFSDDRITAMAFDEEAFWFGTRNGLTRY
                     DLDRRSWRTLSQVDHLSDPRITHLYLDEEYLWVASEAGLSRILKASAGRRDSLVVDII
                     DPRQLGDLRISFLAPQGDTLWVGTEYGLYWYNVSADSGEFYGDGFFPANQEIDAVVCY
                     GDEVWFGTSYGIAGFNARNGEWFAPPAQRLERLGRIFWMEVDGFSVWAAGERGVQRYD
                     REGLRWIRYSMEDGLPAQEIRTLRIDGDYVWFGSSRGLTLFYWNAPYRID"
     gene            12490..13776
                     /locus_tag="PKM23_04545"
     CDS             12490..13776
                     /locus_tag="PKM23_04545"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNY90774.1"
                     /translation="MTYQEAESFLLGREMFGWKLGLERMERFLAGLGAPQEQFDTVHV
                     AGTNGKGSVTAMMGSVLKESGHRCGIYTSPHLESLRERIRVDGLCIAEEAVVGLVERW
                     RPLIEELQCTFFETMTGLALRHFADAGVQSAVIEVGLGGRLDATNVLHPLVSIITSIS
                     YDHMDHLGDTIAAIAAEKAGIIKPAVPCVIGSLPAEAEEVIRARAAELDSPLYAADAL
                     VEVRHLRLTESGSEFSAQTEGARYEGLSLPLPGPHQVANSCIAICASELLGRQGVIPF
                     PPPLKRALAGVDWPGRFEIFLKEPRVITDVAHNPGGFQRQKWMWRHFYPDWQKVLVLG
                     LVKEKDLKGVIGELPRDVAQIYVVPAPTHRALPVAELTGALAEAGLPVVPCGSVQEGV
                     RAAITWARGGTRRVVCITGSHYVVGESLRGIKDLTK"
BASE COUNT         2627 a         3971 c         4580 g         2732 t
ORIGIN      
        1 caccggcatc gccggcccgg ggggtgccac atcggagaaa ccggtcgggc tggtgtatgt
       61 gggctatgcc gggaaggggg gggcggcggt ggaggagcat cgtgtgatct cgggcgaccg
      121 gctggtcaac aaggagcgtt tcgcctatat tgccatgaat ctgctgcgca agcagctctt
      181 caatctgcag acctgatggc gaccctgcgc acattcatct gcatcgagct gtccgaaaag
      241 gtccgggagc ggattgcgga gctgcagcag cagctcaaac cgctgggaag cggcgtcagc
      301 tggacccgtc cggaagggat ccatctcacg ttgaaatttc tcggcgatat cgagtcggct
      361 cgggtggccg acatagccgg tgcagtggag cgagcggctc agggggtggc cccgttcagg
      421 atcacagtgg ccgggacggg cggatttccc aatctgagca ggccgcgggt gctgtgggtg
      481 ggcattgaga gcagcgccag cgcgctgttc gtcgtacagt cacagatcga aatcgagctg
      541 gccagactgg ggtatccccg ggaggaacgg cgcttttctc cccatctgac cctggggcgg
      601 gtaaaatcgc ccgaggcggt gcgggagatc tgccgggagc tgctgcagcg ctcatttgcc
      661 ccggttacct tcactgccgg ctcgatcatc atcatgcgca gtgatcttaa accggatggc
      721 gcactctata cgccgttgag aaccatcgaa ctataaagag caggtgccct tatgccagaa
      781 gagagagatg aaaagcgcaa agcgcttgag ttggccatga cccagatcga ccggcagtat
      841 ggcaaggggt cgatcatgtg gcttggcgag gacaaaaaga ttcccatcga tgtaatttcg
      901 accggatcga tttctctgga tgccgctctg ggagtgggcg gggtgccgcg tgggcgcatc
      961 atcgagattt tcggccccga atcgtcgggc aagaccaccc tggccctgca cgtcatcgcc
     1021 gaggcacaaa aggcgggcgg tctggcggct tttgtcgacg ccgagcatgc cctggatgcc
     1081 cattatgcca atctgctggg tgtggatacg gagaatttgc tcatttccca gccggacacc
     1141 ggcgagcagg ccctggagat caccgaaacc ctggtgcgca gcggggcgct ggatgtggtg
     1201 gtgatcgatt ctgttgccgc cctggtgccg cgcgccgaga tcgaggggga gatgggtgat
     1261 gcccagatgg gtctgcaggc gcgcctgatg tcgcaggcca tgcgcaagct caccgctgca
     1321 atcagcaagt ccaaaacctg tgtggttttt atcaaccaga tccgcgaaaa aatcggcgtc
     1381 atgtttggca gcccggaaac cacgaccggc ggccgcgccc tcaagttcta ctcctcggtc
     1441 cgtctcgata tccgccggac ggcatccatc aaggagggag agaacgtcat cggcaaccgg
     1501 accaaggtga aggtggtgaa gaacaaggtc gcgcccccct tccgggaggc ggaattcgac
     1561 atcatttacg gccagggcat ctccaagatc ggcgatcttc tcgatctggc ggtcaacatg
     1621 aacatcgtcg agaaaagcgg gacctggttc tcctacaagg aggagcgtct cggtcagggc
     1681 cgggagaatg tgcgcaagta cctcgatcag aatcccgagc tgctggcgac gatcgaaaaa
     1741 ggggtgcgtc agaatctggg cctctttccg ggcgcaggcg gggaggaggc cggggagaag
     1801 gccgaaaaga agtaacccgg ccgcgcggga gggagaggaa ccatggctga gatgcgcacc
     1861 gtcacgggga ttgagatgca gaagagggat gcccagcgca tctccatctt tcttgacggc
     1921 gagttcgctt tcggcctcca ccaggatgtc ttgctcgaga gcgggatagc caggggggac
     1981 aggctgagcg aggaacgcat cgaggcgatc ctcgccctcg agcagaagcg gcgggcgaaa
     2041 gagaaggcgc tgcggctgct ttcggtgcgc agccgcagcc gcaaggagct ggccgaccgc
     2101 ctcaggcagg ccaagttcag ccccgaggcg atcgaataca ctctggggga gatggaacgg
     2161 ctcggctttc tcaatgacgg cgactttgcc caagcctggg ggcgcaaccg cacagcgacc
     2221 cgcccggcag gggcctttat gctgcgccag gagctgcgcc ggaaaggagt ggccgaggag
     2281 gatatcgaaa aagggctcca ggcggccttt cgggagagca gcgaggcgga ggtggcccgc
     2341 gagctggcga tccgtcgcaa gcaaaccctc gcggcactgc ccgaggagaa ggcgaggaag
     2401 cggctgcagg attttttact acgccgggga ttcggctggg atctggtcag cgagctgatg
     2461 gaggagtggg agcgcctctg accgaaattt tattccgcac tatcgagttg tataagtaat
     2521 tatgggtatt cgcagggtaa cctggctttc taatgagcaa gagaaggaac cgatccaata
     2581 tatgacttcc caggagatac gtcagagttt tcttgatttt tttaaaagca agaaccatca
     2641 tattgtcccc agtgcaccag tggtgccgca ggatgatccg accctgcttt tcaccaacgc
     2701 cggcatgaac cagttcaagg atgtttttct cggcctgcgg cagcggcagt gggcgcgagt
     2761 cgccgattca cagaaatgca tccgggtctc cggcaagcac aacgatctcg aggaggtcgg
     2821 ccgggacacc tatcatcata cctttttcga gatgctcggc aactggtcct tcggcgacta
     2881 tttcaaggct gaagccatcg aatgggcctg ggagctgctg accggggtct ggcatctgcc
     2941 cgcggacaag ctatacgcca cggtcttcgc cggggatgag ctcgacaagg tgccggggga
     3001 tgaggaggcg gcggaatact gggcgagccg cactgccatt gaccccgcgc atattcttcg
     3061 tttcggcaag aaggacaatt tctgggagat gggcgagagt ggtccttgcg gcccctgctc
     3121 cgaaatccac attgaccgcg gtcccggttt ttgcaccaac accgatcccg gccatgtctg
     3181 cgcggtcaac agtggctgtg cgcgttttat tgagctgtgg aatctggtct tcatccagta
     3241 caatcgggac gaagaggggg ttctcaacac ccttccggcc aggcatgtcg acaccggcgc
     3301 cggtttcgag cgcttggttt cggtgctgca aaagacccgc tccaattatg acatcgatct
     3361 tttcgttccg atcatcgccg agatctcgcg ggtgaccggg cgtccctacg gggaggggga
     3421 agaaggcgtc gcgttccggg ttttatgtga tcacatccgt gccctctcct ttgccatcgc
     3481 cgacggggca atacccggca atgaggggcg gggctacgtt ctgcgccgca ttctgcgccg
     3541 cgccgcccgt ttcggccgcc tcctgggcat gcgcgagccg ttcatctaca aactggtgaa
     3601 gcccctggcc gattcgatgg gggaggccta tccggagctg cggcagcgtc aatcccatat
     3661 tgaacgcgtc atccgctccg aagaggagag ctttgggcgc acccttgacc gcggcatcga
     3721 gatttttgag gagaaagcgg cggcgagccc gggaggggta ctctccggcc gcgatgcctt
     3781 cctgcttcat gacacctacg gcttcccgct cgacctcacc cagttgatgg cggcggagaa
     3841 aaacctcagc gtggacgtgg ccggctttga ggcagagatg gaggagcagc ggcagcgttc
     3901 ttcgcgcgcc cgcgatgtca gctatgagaa gatcgaggtc gatgccgtga tgggggaggg
     3961 ttcgcagttc aggggctacc aggttgacat cctggagacc gagctcatcc attatgaacc
     4021 ggggcggatc gtcctgcgag agagtccctt ttatgcagaa tcgggcggtc aggtcggcga
     4081 ccgcggtgag atcggcaacg accattttcg ttttgccgtc gagaccacaa agcatgtcgg
     4141 ggagcacatt gtccattttg gtgagctcag ggagggaagc gcgcctgttc cgggtgagcg
     4201 ggttgaggcc agtattgatg cggagaggcg gcgggcgacc gagcgcaatc atacggtcac
     4261 tcatcttgtt caccgcgccc tgcggacggt gttgggcgaa catgtccagc aggccggctc
     4321 gctggtggcg cctgattaca tgcgttttga tttcacccat ttcgagaaaa tgagcgcgga
     4381 gcagatccgt gcggttgagg atatagtcaa ccgggagatc ctgcagaatc gcccggtgca
     4441 ttggcaggtg atgccgatcg aggaggccaa ggcgctcggg gcgatggccc tcttcggcga
     4501 gaaatatggg gacgaggtgc gcatggttga ggtgcaggat ttttcccgcg agctttgcgg
     4561 gggcacccat gtacgcgcca ccggggagat cggccttttt gtcctgcgcg gggaatcggc
     4621 catcgccgcc ggggtacggc ggctggagtg cctcaccgga gagtccgccc gcgcatggct
     4681 gcagggcagg gcggacatcg ccgcgcagag cgcggtcctg ctgggctgcc gtgacgagga
     4741 ggtggttgaa cggctggagc ggcttcagca ggagcgcaag gcgttggagg gggagatgcg
     4801 caaacttcgc cactccactt cccgggatat tgtcggcgaa ctggtgcgcg gggcgcacaa
     4861 ggtcgctggc atcagtgtag cggcggcgca gatcgaggct gcgggggttg acgaactgaa
     4921 gcagacagcg gatctgctgc gtgaaggcct gcagagcggc gtcggcgtac tggcggctgt
     4981 tattggtgat aaggtccagt tcgtctgcgt cgtgaccaaa gatctggtcg aggggcgcaa
     5041 gctcaaggct ggggatatcg tccgcgagat tgcggcggtt gcgggtggca gcgggggcgg
     5101 cagcccccac atggctcttg ccggggccag ggatgccggc aagatcgccg aggccctgaa
     5161 tggggtggag aagatcatcg ctgaaatgat cacaaggttg gagcccggcg ctacccaggg
     5221 ctgatcgtca ctcaccaact aatatgaggt agtccgtgaa ggtttatgag cagcttctgc
     5281 agatcaggga ggaaaagggc gccggctatc tggtgttgat tgatccggac aagcagagca
     5341 tgcaggaggc gatcgagctg gcacgggtgt gcagcgagag cggggttgac ggtctgctgc
     5401 tgggcggcag tctgatgttc acccatattt ttgatgatct gatcaagggc atcaagaccg
     5461 cctctccact gcccctgatc atttttccgg gcagcacgcg tcagctctcc ccctatgcga
     5521 atgccatact ttatctttcg ctcatcagcg ggcgcaatgc ctattacatc atcggcgacc
     5581 aggtgctggc cgcgccgata atcaagtcga tgaatctgga agccatttcc accgcttata
     5641 tgctgatcga atcgggaaat atcacttccg cacaatttct cagcgatacg cgcccgatac
     5701 caagggagaa gcccgacatc tccgtcgccc acgcgatggc tgcggagctt ttgggaatgc
     5761 gcatgatcta tctggaggcg ggcagcgggg cgcatctctc ggtgcctgaa gaacatatcg
     5821 ctgccatctg ccgctacacc tcccttcctg tcatcgtcgg cggcggcatc cgcgcccctg
     5881 aagaggcggc gcgcaaggtg gcggcagggg cgagttttat cgtgaccggc aatgtcatcg
     5941 aaaagaaccg cgatcgcgcc ctgatcagcg cctttgccga ttccattcac accaggtagt
     6001 actaatcagg gcggacggga ggtttatggt tctggtcgat acacatgcgc atttgctgcc
     6061 cttctgggat gatggagcgg acaggtggga tacggcactg gggatgctgc gcgcagcgga
     6121 ggaggacgag atcggggcgg tggtctgcac cccgcacatc ctttcgccca aggacatgga
     6181 gcatgaggat gccctctggg tgcgctacga agagctggtg gggcgttccg ccgaggcggg
     6241 tctgaaagtg aagattcaca tgggcagcga gcttttcgcg catcccggca tcgacctgaa
     6301 gcgcagaata gccaccccgg cgcagaacgg ccgctatttt ctcgtggagt ttccaatggg
     6361 ttcgatgccc gattttgttc tcaagggctt tttcacccag attggcaagg agtacacccc
     6421 ggtcattgcc catccggagc gttatgcaag gatcatccag aatccccggg aggccatgat
     6481 cctggtcgag aagggggcac ttttacagat caacgccggg agtctgcttg gggtctttgg
     6541 cggagcgatc aaggagacgg cggtcaaact gctcgatgcc aacctggtgc acatggtcgc
     6601 ctcagacgcc cacgacctca aggcgcggcc gctgcggctg cagccggctt ttacagtggt
     6661 ggcggaaaag tacggccgcg agaaggcgca gaggcttttt gctgacaatc ctgcccgcat
     6721 tttgcgcggg gaggatgtga tcgcggatcc tccccgtccg atcgaggagg cccccaagcg
     6781 cggcttctgg cagcggctgg agatcatgac caaaaaatag cgcaggatat ggaaagactg
     6841 acttttatag aagagcagat gagagagagc gtggagctgt tcgaggccat ccgccgcgaa
     6901 atggggccgg cgatcagccg cggtgcggag atgatgatcg acaccctgcg cagcggcggc
     6961 aagctcctca tctgcggcaa cggcggcagc gctgcggatg cccagcattt cgccgcggag
     7021 ctggtgggcc ggctgcagcg ggagcgcaag gccctgccgg cgatcgcgtt gaccacggac
     7081 acctccattc tcaccgctgt gggtaatgac tatggttttg ccaaaatttt ccgcaggcag
     7141 gtagaggcct ggggtcagcc gggcgatctg ctgctggcga tcagcaccag cggcaattca
     7201 cccaatgttc tggaggctat cgctgcggct cgggcgcaag gcatgcaggt tctctccctg
     7261 gcgggacgaa gcggcggcga gatggccggc ctctccgatc tggcgttggt gatgccggcg
     7321 cgcagctccc agcacattca ggaggggcat atcgccatca tccacatctg gtgcgccctt
     7381 gttgaggaga gtctgtgtcc tcctgtatcc taatccagag tcatccatcg attgaatttt
     7441 cttaattctg ccatattgat ggggctggct gcagtggcct tgcccatcct catccacctc
     7501 ttcaccagag cccgggccaa acccatcccc ttcagctcac tgcgtttcct caagcagctg
     7561 cggaaccaga aaatccggcg catcaagata cgtcagatcc tgctccttct tttgcgcacc
     7621 ctggcggtgc tttttcttgt gctcggcttc gcccgcccga cctgccgcac ccaatccggt
     7681 tccggggccc gttcccgcac aagtgcggtc cttctgctcg acaactcggc aagcatggcg
     7741 ctcgaagagc agggggagag cctctttgcg gcggccaggg aagcgggggc gcgcatcatc
     7801 gagctgatgc agccgggcga cgagctctat ctttgcacga ctaccgatac gttagaaggg
     7861 acggagcggc gggcctttca tgattttcag gccttccgcc gccggctcga ggccacaccc
     7921 ctcagtttca gggcgactga tatctcggcg gggatgcgtt ttgcacagaa cctgcttcaa
     7981 tccgcccaca atgtaaacaa ggaactttac ctgctctcgg acatgcaggc gaccggcttt
     8041 gctgctgatt ccctgccggc gcgggaattc aatccgcggc agtacgccgt gccgctgctc
     8101 gcttcccatc ccgccaacct ggccgtgacc ggcgtccggc tgcgctccgc cattctggaa
     8161 agaggcaaga cggtggaggt ggaggtgacc ctggccaact ccggccagga gcccgggcgc
     8221 accaagctgg tgcagctctt catccatggc cagcgtgtgg cccaacggat ggtctcgctg
     8281 gagaggggga cgacggcgca ggagctcttc aggttcatcg tcgaccgcga cggctggatc
     8341 gagggctatg tccagctcga agatgacgct ctgatggagg ataaccttcg ctattttacc
     8401 ttctatgtgc cggaacggct caatgtcggg gtgatcggcg agaggaccgc cggcagtgaa
     8461 ctgctgcagc tcgccctgca atcttcggcc gattcgagtg cctttttgcg ccttttcacc
     8521 gctgtgcctg aacgctcctc cggtctggcc atcgattccc tccagctgct tttgcttcac
     8581 gatgtcagcc gcctgcccga cgcggtggtg gagaggattg gggcgtggca cagtcgcggg
     8641 ggcgggatca ttctggtgct gggccggcgc acggacatcg gctggtacaa cgcccgtctc
     8701 gccccggtcc tggggctcag ccccgtgctg gccgcgttcg gcgagggggg tcatttttca
     8761 cttggccgtg tcgatggcac tcatcctgta ttttctggta tctttgaggg agcggagatc
     8821 cagttcgccc gcccccaatt caatttcgcc tgtcgcgtcg cttccgcccc cgaccagcat
     8881 gccctgctct ccttcagcac cggggatccc tacctgtatg aagtcagacg cgatgagggc
     8941 gccctgctgg ttttcacctc cagttttgag ccggaggtga gcgacatggc gtacagaacc
     9001 atctttgctc cccttctcca tcgcagcatc agctatctgg ctgcgcatgg ccagaaccgc
     9061 ggggtcgagc tttctgcggg cgaggtttta cgttaccggc ttgccggtca ggagctgcag
     9121 aagcagctgg aaatacaccg gcctgacggc ggagtggatc ttgtccaccc cgggatcacc
     9181 gcatcggggg cgtggatcga ccacgctgcc accggaattc cggggctcta ttcactgaga
     9241 gcagatggca aacggcttgc ggtatgggcg gtcaatattc cggcccggga gctggatctg
     9301 agaagggcgg agagggaact cgtcgaggat tcccaccacc tccactgggt ggatgatccg
     9361 gcccgcatcg accagttcat ccgcgaggag cggatcggac gggagtactg gcgggagctg
     9421 ctgctggccg ggctgctgct tctgctgctg gagatggccc tctatcgcga gaagggagaa
     9481 ctcccgtcgg aagaggagag tcaggagatg cggaggggat catgaaggaa tcccaggaga
     9541 gagagcgagc cgtcatcgtc ggactaatca ccagggagac cccctcctgg cgtgtcgagg
     9601 actatctgga cgagctggaa caactcgctg atacggcggg ggccgaggtg atcgagcggg
     9661 tggtgcagga gcgggagcgt cgggaccccg ccttcatgat cggccggggc aaggcggagg
     9721 aactcgcccg aactgcccgc taccaggatg ccgatctgat catttttgac gatgatctca
     9781 ccccggcgca ggcgaaaaac atcgaaaagc tgtgcggcgt caagatcgtc gacaggagcg
     9841 ccttgatcct cgacattttt gccaaacatg cccgcacccg cgaggcccgc acccaggtgg
     9901 agctggcgca gctgcagtat ctgctgcccc gcctcaccgg gcagtggcgt catctcgagc
     9961 gtcaagtggg cggtatcggg gtgcgcggtc ccggggaaac ccagctcgag gtcgatcgcc
    10021 ggctggtgcg caaacgggtc gccgttttac aaaaggagtt ggaaaagatt gccggccagc
    10081 gcgagatccg ccgcaagaac cgtcgtgatt ttttcaaggc ggctctggtc ggctatacca
    10141 atgtcggcaa atcgaccctg ctcaatgccc tgaccgaggc cggagtgctg gttgaggacc
    10201 gccttttcgc caccctagat gccacagtcc gcaccctgcc catggacaac cgccgccggc
    10261 tgctgctgat cgacacggtc ggcttcatcc gcaagctgcc gcaccacctg gtcgcctctt
    10321 tcaagagcac cctggaggag tcggctgatg ccgatcttct tctccatgtc atcgatatca
    10381 gtcaccccaa ttttggcgag cagatgaatg tggtggacaa ggttctcaag gagctcaacc
    10441 ttggggggag gccggtgctc aaggtcttca acaagatcga ccagtttgac cagtccggtg
    10501 tggtcgccag gctgcgggag accgagcagc cctgtgtgtt cgtctcggca gcgcgcggca
    10561 tcggcctgcc gcagctggtg gagcggctgg cggagttcgc cgatgccgcc tttgtcacgg
    10621 atgaattggt gcttcccctg gaaaaggcct cccgggttcc caaactgtat gaggtggtcg
    10681 aggttctgaa cacctcctac ggggaggagg agatggcggt gcaaatccgc ttcgcacgcc
    10741 aggaggagcg gcgggtgcgc agccaggttg cgaaaatact gcagatggat gaggaagatg
    10801 gctctgcgca tgaatagagg ggctagctgg ttgacggcgg tgctgatttt gctctgggtg
    10861 gcgggagcag cagcccagag cgaggggcgc atccgcggca ccaaccgcca ttaccgccac
    10921 ggagattgga caacctggtc ctcgatgcgc catattcgcc acctctgcct ttcacaggag
    10981 cgtctctatc tggccacgac cggcggaatc ggctgcttca attttttcag ccagcagtgg
    11041 gaggagcccc atacggtcag cagcggtctg gccagcgctg atatcggact ggtggcctgg
    11101 gacgaaaaca gcgggttttt gtggtgtacg cagagcgagg gcatcagcta tctgggtccg
    11161 gcttccgggg tgtggaccaa tcttttttac gacgagatcg gtttttacga ccgtgagtcc
    11221 gtaagctcca ttggtttcgg cgtcaatcat caaatctacc tggtcaccag ccgggggcgc
    11281 agcctggccg gccacagcag cggaggcggc ttcgagtggg gcgccactcc gcggccggag
    11341 gaggaggtca aatggttcgg ggaggccgct gctgtcgatc cacccccgcc ccatctcttt
    11401 ctacccccgg gttattatta tgacgaacag gaaagggtga tcaccgacag tcatggcagg
    11461 cgttatccac tcacctgctg gctgcgcgac gcctggaaca ccctctggat cggcacctgg
    11521 gggctggggg cggcgcgcgt cgacctggtc tccgcccgat ttgaggccct gccctacggc
    11581 ctgtgggatg acgctgtcga cgtcctcgcc tttgatcagg gcgcactctg ggccggcggc
    11641 gatcaggggc agacgggggg aggagggttg acgagatggg agatcggcat ccgcaatccc
    11701 gaatactatg aaccacgcta tatcaccggt ttcagcgacg accgcatcac tgccatggcc
    11761 tttgatgagg aggccttctg gttcggcacc cgcaacgggc tgacgcgcta cgatctcgac
    11821 cggcgcagct ggcgcaccct gagccaggtt gatcaccttt ccgatccgcg catcacccat
    11881 ctctatctgg acgaagagta tctctgggtg gcgagcgaag cggggctgtc gcgcattctc
    11941 aaggcgagcg caggccggcg ggactccctg gtcgtcgata tcatcgaccc gcgtcagctt
    12001 ggcgacctga ggatctcatt tctcgccccg cagggggaca ccctctgggt gggcaccgag
    12061 tatggattgt actggtacaa tgtgagtgcc gacagcggcg aattctacgg tgatggcttt
    12121 tttccggcga accaggagat tgatgctgtg gtctgctatg gagatgaggt ctggttcggc
    12181 accagctacg gcatcgccgg attcaatgcc aggaacggcg aatggttcgc tccgccagcc
    12241 caacgtctgg agcggttggg ccggatcttc tggatggagg tggacggttt ttcggtctgg
    12301 gcggccgggg agcggggggt gcagcgttat gaccgtgagg ggctgcgctg gattcgttac
    12361 agcatggagg acggcctgcc cgctcaggag atccgcaccc tccgcatcga tggggattat
    12421 gtctggttcg gcagcagccg cggcctgacc cttttttact ggaatgcacc ttaccggatc
    12481 gattgaatca tgacctatca agaagcggaa tccttccttc tggggcggga gatgttcggc
    12541 tggaagctgg ggctggagcg catggagcgt tttctagccg gactcggagc gccgcaggag
    12601 cagttcgaca ccgtacacgt cgccggcacc aacggcaagg gctcggtcac tgccatgatg
    12661 gggtctgttc tcaaggagag tggccatcgc tgcggcatct atacctcgcc ccatctggag
    12721 agcctgcgcg agcgcatccg cgtcgacggc ctctgcatcg ccgaggaggc ggtggtggga
    12781 ctggtcgagc gctggcggcc gctgatcgaa gagctgcagt gcactttttt cgagaccatg
    12841 accggcctgg ccttgcgtca tttcgccgac gccggggtgc agagtgcggt gatcgaggtc
    12901 ggactgggcg gccgtctcga tgcgaccaat gtcctgcatc ccctggtgtc gatcatcacc
    12961 tcgatcagct acgaccacat ggatcatttg ggggatacca tcgcggccat cgcagccgaa
    13021 aaggcgggca tcatcaagcc ggcagttccc tgcgtgatcg gttcactgcc ggcggaggcg
    13081 gaagaggtga tccgcgcgcg ggcggctgaa cttgacagcc cactttacgc ggccgatgcc
    13141 ctggtcgagg tgcgccacct gcgtctcacg gaatcgggct cggagttttc ggcccagacg
    13201 gagggtgccc ggtacgaagg gctatcgctg cccctgccgg gtcctcacca ggtggccaat
    13261 agctgcattg caatctgtgc cagtgagctg ctcggccggc agggggtgat tccctttccc
    13321 ccgcctttaa aacgggcgct ggcgggagtc gattggcctg gccgctttga aatcttcctc
    13381 aaagagccgc gggtgatcac cgatgtggcc cacaatccgg gcggttttca gcggcagaaa
    13441 tggatgtggc gtcatttcta tccggattgg cagaaggtgc tggtcctcgg cctggtcaag
    13501 gagaaggacc tcaagggtgt gatcggggaa ctgccgcggg atgtggccca aatctacgtc
    13561 gtgccggcgc cgacccatcg tgcgctgccg gtggcggagc tcaccggcgc gctggcggag
    13621 gccgggctgc cggtggtgcc ctgcggttcg gtacaggaag gggtccgggc ggcgataacc
    13681 tgggcgaggg gtggaacaag gcgggttgtc tgcatcaccg gctcccacta tgtggtgggt
    13741 gagagcctga ggggaataaa ggacttgaca aaatgactat ttcttcctaa attatgcagg
    13801 aaccaatcct gctcgacccc attttaacac accttattct tcataaagcc gccaatcact
    13861 gtgcaccttt tcatctcatg aagagagaat ccacccaact attacaacgg
//
LOCUS       DAOPWU010000053        13822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4522_length_13822_cov_4.251180, whole genome shotgun sequence.
ACCESSION   DAOPWU010000053 DAOPWU010000000
VERSION     DAOPWU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4522_length_13822_cov_4.251180"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /gene="rpsS"
                     /locus_tag="PKM23_04550"
     CDS             <1..255
                     /gene="rpsS"
                     /locus_tag="PKM23_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNY90775.1"
                     /translation="IDANLLAKIDELNRINQKKVIKTWARRSTIPPEFIGHTLAVHNG
                     NKFVPVFISENMVGHKLGEFALTRTFRGHTEKKSEKSSRP"
     gene            280..630
                     /gene="rplV"
                     /locus_tag="PKM23_04555"
     CDS             280..630
                     /gene="rplV"
                     /locus_tag="PKM23_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNY90776.1"
                     /translation="MEAKAVAKWVRNSPLKVRRVANLVRGKVVQEALNILHFSDTAAS
                     EPLEKAIRSAVANMLNKEEASKMSPEDLYIKELRIDEGFMLKRFRAASMGRATRIRRR
                     TCHISVIVADKPVN"
     gene            651..1283
                     /gene="rpsC"
                     /locus_tag="PKM23_04560"
     CDS             651..1283
                     /gene="rpsC"
                     /locus_tag="PKM23_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNY90777.1"
                     /translation="MGQKTNPKGFRLGVIRTWDSNWFDERDFAKKLSEDIMLRRYVRN
                     RLQKASISKIEISRTPKQITLTIHTARPGIVIGRKGAEVDKLREELQRVTGKDVQLNI
                     NEIKRPELDAYLVAESIANQLEGKVSFRRAMKKAMQSTMRMGAEGIRICCSGRLGGAE
                     MARVEQYKEGRIPLHTLRADIDFARATSRTTYGAIGVKVWICRGEVIGEQ"
     gene            1324..1743
                     /gene="rplP"
                     /locus_tag="PKM23_04565"
     CDS             1324..1743
                     /gene="rplP"
                     /locus_tag="PKM23_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNY90778.1"
                     /translation="MLMPKRIKFRKHQRGRMRGLAVRGSLLAFGEYGLKAVEPHWITA
                     RQIEAARIAMTRYIKRGGKIWIRIFPDKPCTKKPAETRMGKGKGAPEYWVAVVKPGRI
                     MFEIAGVDENTAREAMRLAAHKLPVKSKFVSATELGE"
     gene            1747..1947
                     /gene="rpmC"
                     /locus_tag="PKM23_04570"
     CDS             1747..1947
                     /gene="rpmC"
                     /locus_tag="PKM23_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNY90779.1"
                     /translation="MNKTEELRKLSLKELDMQLEDALLEMSNLRIQHSTRQLDNPLRI
                     KRVRRDIARLKTIINEKSQVNA"
     gene            1973..2227
                     /gene="rpsQ"
                     /locus_tag="PKM23_04575"
     CDS             1973..2227
                     /gene="rpsQ"
                     /locus_tag="PKM23_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNY90780.1"
                     /translation="MERKQRKVKIGIVASDKMNKSRVVTVQRFVKHPLYKKYVKKTSK
                     FMAHDESNTSHVGDVIKIMETRPLSARKRWRLVDVIERAK"
     gene            2261..2629
                     /gene="rplN"
                     /locus_tag="PKM23_04580"
     CDS             2261..2629
                     /gene="rplN"
                     /locus_tag="PKM23_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNY90781.1"
                     /translation="MVQEYTRLNVADNTGAKKVMCIRVLGGSKRRTASIGDVIIVSVK
                     QAIPNSSIKKGDKSKAVIVRTKKEKRRPDGSYIRFDENAAVLINDAMEPKGTRIFGPV
                     ARELREKQFMKIVSLAPEVL"
     gene            2653..2988
                     /gene="rplX"
                     /locus_tag="PKM23_04585"
     CDS             2653..2988
                     /gene="rplX"
                     /locus_tag="PKM23_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNY90782.1"
                     /translation="MRVRKNDQVLVIAGNYNGKKGKVLKVFPEKSQVIIEGVHFIKKM
                     TRPSQKNPQGGIVEKEAPIHVSNVMVICPKCNTPARMGSKQVFDEAKSRKGHVRVCRN
                     CNEMLVTQS"
     gene            3016..3576
                     /gene="rplE"
                     /locus_tag="PKM23_04590"
     CDS             3016..3576
                     /gene="rplE"
                     /locus_tag="PKM23_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNY90783.1"
                     /translation="MAEEKYKPRLIDVYAKEVRPQLQKRFNYKNVMQVPRIEKIVLNM
                     GVGDATENAKNVDVALEEMTKIAGQKPVIRRAKKAISNFKLRAGMAIGCSVTLRRWHM
                     YEFLDRLISIAIPRVRDFRGLALKFDGRGNYNMSIKEQIVFPEIDYDKVEKTRGMNIT
                     IVTTAKTDEEALELLTAFGMPFIRRS"
     gene            3601..3786
                     /locus_tag="PKM23_04595"
     CDS             3601..3786
                     /locus_tag="PKM23_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011077818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNY90784.1"
                     /translation="MARKALVVKSKRTPKFAVRAYNRCSRCGRPRAFYRKFGICRLCF
                     RDLALEGLIPGVKKASW"
     gene            3853..4251
                     /gene="rpsH"
                     /locus_tag="PKM23_04600"
     CDS             3853..4251
                     /gene="rpsH"
                     /locus_tag="PKM23_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNY90785.1"
                     /translation="MSKTDPIADYLTCIRNATRANHPKVDIPASRLKQEITKILQEEA
                     YIKNHVIINDGKQGVIRIYLKYDNDNRSVISGLRRISKPGFRRYVNHKAVPRVFNNLG
                     IAIVTTSEGVMTGQEARKRGVGGEVLCYIW"
     gene            4275..4811
                     /gene="rplF"
                     /locus_tag="PKM23_04605"
     CDS             4275..4811
                     /gene="rplF"
                     /locus_tag="PKM23_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNY90786.1"
                     /translation="MSRVGKAPISIPDGVQVDVTNNLVTATGPKGTLAYQVDPRLIVK
                     MENGTLEVQRPNDDKIVRSLHGLTRTLVNNVVEGVSKGFEKKLEIIGVGFRAEMKGTH
                     LLLSLGYSHQIIVAPPEGITLATEGNNIVVVRGNSKELVGQIAAKIRSLRKPEPYKGK
                     GVRYLGETVRKKAGKTAA"
     gene            4899..5273
                     /gene="rplR"
                     /locus_tag="PKM23_04610"
     CDS             4899..5273
                     /gene="rplR"
                     /locus_tag="PKM23_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNY90787.1"
                     /translation="MADKSYTKVEARHRRHSHILKTLRGSAERPRLVIFRSNKHIYAQ
                     VVDDLSRTTLFAASTLTPAIRDVVAKAKSKKEQAMLVGKHLAEIAKDKEVTRIVFDRG
                     GYLYHGRVKALADGAREGGLEF"
     gene            5311..5844
                     /gene="rpsE"
                     /locus_tag="PKM23_04615"
     CDS             5311..5844
                     /gene="rpsE"
                     /locus_tag="PKM23_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNY90788.1"
                     /translation="MEKINPGEFELTERVVQVNRVAKVVKGGRRFSFNAIVVVGDGHG
                     RVGIGLGKANEVTDSITKGADAAKKALIRVPVVNGTITHEIEGKYGAGRVFLKPAAPG
                     TGVIAGGAVRAILESAGYTDILTKSRGSANPHNVVKATIAALENLMDVAGVSRKRGLQ
                     VMQVFKTVPKPKVGANE"
     gene            5862..6062
                     /gene="rpmD"
                     /locus_tag="PKM23_04620"
     CDS             5862..6062
                     /gene="rpmD"
                     /locus_tag="PKM23_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNY90789.1"
                     /translation="MNKKKKEAKQIKITLVRSTIGSIQKHKATAIALGLRHPNNSVVH
                     YDTPIIRGMVDNIRHLVKVEEL"
     gene            6089..6523
                     /gene="rplO"
                     /locus_tag="PKM23_04625"
     CDS             6089..6523
                     /gene="rplO"
                     /locus_tag="PKM23_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNY90790.1"
                     /translation="MNLANLTYAEGAVKDRKRVGRGGSRGKQSGRGSNGAGSRSGSKR
                     RAWFQGGQMPIQRQLPKRGFKNLNRIEFQVVNVGVLDKLPGGEELTPALLFELGAIGK
                     KDTPVKVLGDGEVTKKLTVSANAFSKSAQEKSEAAGGRITKI"
     gene            6520..7847
                     /gene="secY"
                     /locus_tag="PKM23_04630"
                     /pseudo
     CDS             6520..7847
                     /gene="secY"
                     /locus_tag="PKM23_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
     gene            7869..8513
                     /locus_tag="PKM23_04635"
     CDS             7869..8513
                     /locus_tag="PKM23_04635"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNY90791.1"
                     /translation="MRVIFLGAPGTGKGTQAVWLSDKFTIPQISTGDILRKAVADGTE
                     LGKKADVVMKSGGLVSDDLIIALVRERLDQPDCRPGFILDGFPRTIAQGESLDLMLGR
                     DGAIQSVIFFEVSEAEIVRRLTSRRTCSGCARNYNMISDPPPASGLCGSCGGRIIQRP
                     DDSEETVRKRLQVYDEKTAPLKEFYNKQKKLYILRAEQPVERVREELAALLSGL"
     gene            8520..9266
                     /gene="map"
                     /locus_tag="PKM23_04640"
     CDS             8520..9266
                     /gene="map"
                     /locus_tag="PKM23_04640"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNY90792.1"
                     /translation="MINIRSEKEIDLIRQSGRILAGALHLAESSIAAGVVTGELDQEI
                     AAYIRSRGGKPAFKGYNGYPANTCISIDEQVVHGIPGHRALQEGEIVSVDVGVLLNGY
                     YSDAARTFKVGAVSAEKERLMEVTLASLGKGIAAAVDGNRLSDIGHAVQSHVEAAGFS
                     VVRELVGHGIGQAMHEEPQIPNYGAPDRGPRLREGMVFAIEPMVNAGDWQVKTLEDQW
                     TVVTADGKPSAHYEHTVVIRKGAAEILTLE"
     gene            9278..9496
                     /gene="infA"
                     /locus_tag="PKM23_04645"
     CDS             9278..9496
                     /gene="infA"
                     /locus_tag="PKM23_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNY90793.1"
                     /translation="MPKEASIKIDGVILETLPNASFRVELENGHKVLAHISGKMRMHF
                     IKILPGDKVTVELSPYDLSRGRITYRFK"
     gene            9531..9647
                     /gene="rpmJ"
                     /locus_tag="PKM23_04650"
     CDS             9531..9647
                     /gene="rpmJ"
                     /locus_tag="PKM23_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNY90794.1"
                     /translation="MKVRSSVKKICDSCKIIRRKGTIRVICKKNPRHKQRQG"
     gene            9674..10045
                     /gene="rpsM"
                     /locus_tag="PKM23_04655"
     CDS             9674..10045
                     /gene="rpsM"
                     /locus_tag="PKM23_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNY90795.1"
                     /translation="MARISGIDLPRDKRIEVALTYIYGIGLTTSREILEKTRINPDTR
                     VRDMQPDEVAKIRDLIANDYKVEGALRSETQMNIKRLIDIGCYRGMRHRRNLPVRGQR
                     THTNARTRRARRGGVGIRKKK"
     gene            10085..10474
                     /gene="rpsK"
                     /locus_tag="PKM23_04660"
     CDS             10085..10474
                     /gene="rpsK"
                     /locus_tag="PKM23_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNY90796.1"
                     /translation="MANPKKKTTKKREKVEANGVAHIRVTFNNTNITLTDMYGNVISW
                     ATAGRSGFKGSRKSTPYAAQMAAESAAKEAIDLGLRRVEVLVKGPGSGREAAVRSLQA
                     AGLEISSIKDTSPIPHNGCRPPKRRRV"
     gene            10573..11202
                     /gene="rpsD"
                     /locus_tag="PKM23_04665"
     CDS             10573..11202
                     /gene="rpsD"
                     /locus_tag="PKM23_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNY90797.1"
                     /translation="MARDTGADCKLCRREGMKLFLKGTKCTSDKCPFDKKGYAPGQHG
                     RTRRFKQSEYSLQLREKQKVKRIYGLLESQFHTYFERAERLRGITGENLLRLLETRLD
                     NVVYRLGLAPSRTTARQLVRHRHVLVNGELVDIPSYQLKAGDEIRIREKSRQLEIIHS
                     AMKRVRDGKMVAWLSLDKAGMLGTILEQPARDAIPVDVNEKLIVELYSK"
     gene            11265..12251
                     /locus_tag="PKM23_04670"
     CDS             11265..12251
                     /locus_tag="PKM23_04670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNY90798.1"
                     /translation="MALPSFQMPESIELDESTYTNTYGKFIVQPLERGFGATIGNAFR
                     RVLLSSIPGAAITTFRVDGVLHEFSTIPGVKEDVSTIVLNLKQVRLKLIDKKPDKISI
                     HLKGPKTFTARDIQEQSTDFEVLNPDWHIATLNADADFTIELRIGRGRGYVPAEENRL
                     PDLPLGTIPIDAVFTPIVRVTYTVENTRVGQRIDYERLILEISTDGSITPDDALSFAG
                     KILRDHIQLFINFDIDTEEEESQEVDEESIRIRKLLKMSVDELELSVRSHNCLMAANI
                     KTIGDLVRRDEQEMLKFRNFGRKSLSELAKILEEKGITFGMDVDKYLRKGDD"
     gene            12321..12818
                     /gene="rplQ"
                     /locus_tag="PKM23_04675"
     CDS             12321..12818
                     /gene="rplQ"
                     /locus_tag="PKM23_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNY90799.1"
                     /translation="MRHGKSGRTLGKTHSHRKAMMNNMAASIISHKSIITTTPKAKEA
                     RGVVERLITFAKKNDLAARRMVMRSIKDKELVRELFEEIGPKYADRNGGYTRVVKLGN
                     RQGDGASMSIFELVGYEGAKAAKIEKQRQKRQEKAAKKKKEIEEAARQEQQQPPQEEE
                     GGEKK"
     gene            13104..>13822
                     /locus_tag="PKM23_04680"
     CDS             13104..>13822
                     /locus_tag="PKM23_04680"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNY90800.1"
                     /translation="MKRLFLPAALLLLVLVLACTNEEPLPSGLDALHRANTDSLRVVT
                     IPAQEAAWFRREVNPGLTASLLVGRHDNITARALLKFTGFSTIDTASVNAATLTLTQK
                     GRWGAGGAFTCTVHKVTAAWAEKSVQWSEISGGYELAPLASFEILAQDSATYAVELPP
                     AVVNDWIGGSDKNGLLLDFTGADMAAELGSVDVSSGGYPTLKLAFTTKKGEADTVTVS
                     PAEDATLFTWDQLPPVDVVERG"
BASE COUNT         3151 a         4222 c         3937 g         2512 t
ORIGIN      
        1 atcgatgcca acctgttggc caagatcgat gagctcaacc ggatcaatca gaagaaggtg
       61 atcaagacct gggcgcgacg gagcaccatt ccgcccgagt tcatcggtca cactctggct
      121 gtgcacaatg gcaataagtt cgtcccggtt tttatttccg aaaacatggt cggtcacaag
      181 ctgggtgagt ttgccctgac ccgcactttc cgcggccata ccgaaaagaa gtcggaaaaa
      241 tcatccagac cgtaagcgct agttttggag aatctttaca tggaagcaaa ggcggtagca
      301 aagtgggtcc gcaactcccc cttgaaagtg cggcgcgtgg ccaatctggt gcgcggcaag
      361 gtggtccagg aggcgctgaa tatcctccat ttcagcgaca ctgcggcctc cgagcctctt
      421 gagaaggcca ttcgctcggc ggtggccaat atgctcaaca aggaagaggc cagcaagatg
      481 tcgcccgaag acctctacat caaggagctg cgcatcgacg agggctttat gctcaagcgg
      541 ttccgcgcgg cctcgatggg ccgggccacc cgtattcgca gacgcacctg tcatatttct
      601 gtgatcgttg cagacaagcc agtcaactag atattttaca ggagattatt ttgggacaga
      661 agacaaatcc taagggattt aggctgggtg tcatccggac ctgggattcc aactggttcg
      721 atgaacgcga tttcgccaag aagctgagcg aagacatcat gctgcgccgc tacgtgcgca
      781 accgtctgca gaaggccagc atctccaaaa tcgagatcag ccgcacgccc aagcagatca
      841 cgctcaccat tcacaccgcc cgccccggca tcgtcatcgg ccgcaagggc gccgaggtgg
      901 acaagctgcg cgaggaactg cagcgggtga ctggcaagga tgtgcaactg aatatcaacg
      961 agatcaagcg ccccgagctg gacgcctatc tggtcgccga gtcgatcgcc aaccagctgg
     1021 aaggcaaggt ctctttccgg cgtgcgatga aaaaggcgat gcagtcgacc atgcgcatgg
     1081 gcgccgaggg catccgtatc tgctgttccg ggcgtcttgg cggggcggag atggcgcggg
     1141 tcgagcagta caaggagggg cgtattcccc tgcacaccct gcgcgcggat attgatttcg
     1201 cccgtgccac ctcccggacc acctacggcg ccatcggcgt caaggtctgg atctgtcgcg
     1261 gcgaagtgat cggcgaacaa taatcatcgg atctgatacc ttttatattg gagtactgca
     1321 gtcatgttaa tgcccaagcg cataaaattt cgcaagcacc agcgcggccg catgagaggg
     1381 ctggcggtgc gcggctcgct gctcgctttt ggcgagtatg gtctgaaggc ggtcgaaccg
     1441 cactggatca ccgcccgcca gattgaggcc gcccgtatcg cgatgacgcg ctatatcaag
     1501 cgcggcggca agatctggat ccgcatcttc ccggacaagc cgtgcaccaa aaagccggcc
     1561 gagacccgca tgggtaaggg aaaaggcgcc cccgaatact gggtggcggt agtcaagccc
     1621 ggccgcatca tgttcgagat cgccggcgtg gatgagaaca cggcgcgcga ggccatgcgt
     1681 ctggccgcgc acaagctgcc ggtcaagtcc aagttcgtct ccgccacgga attgggagag
     1741 taaaccatga acaagaccga agaattgcgc aaactgtcgc tcaaagagct cgacatgcag
     1801 ctggaagatg ccctgctgga gatgtccaac ctgcgcatcc agcactcaac ccgccagctc
     1861 gacaatcccc tgagaatcaa acgggtgcgc cgggatatcg cgcggctgaa gaccatcatc
     1921 aatgaaaaga gccaagtcaa cgcctaaggc cctactgcca ttggagagtt ttgtggaaag
     1981 aaaacagaga aaagtcaaga tcgggattgt cgccagcgac aagatgaaca agagccgcgt
     2041 ggtgacggtc cagcgctttg tcaagcatcc cctgtacaaa aagtacgtca aaaagacgtc
     2101 caagttcatg gcgcacgacg agagcaacac cagccatgtc ggggatgtga tcaagatcat
     2161 ggagacccgc cccttgagcg cccgcaagcg ttggcggttg gtggacgtca tcgagcgtgc
     2221 caagtaacag tttgcgaatt cgaagcgaaa ggtctgagtc atggttcagg aatatacccg
     2281 gcttaatgtc gcggacaaca cgggcgccaa gaaggtgatg tgcatccggg ttctcggcgg
     2341 ctcaaaaagg cggacggcct cgatcggcga tgtcatcatc gtctctgtca agcaggccat
     2401 ccccaacagc agtatcaaga aaggggataa gagcaaggcc gtgatcgtgc gcaccaagaa
     2461 agagaaacgc cgtccggacg gctcttacat ccggttcgac gagaacgcgg ctgtgctgat
     2521 caacgatgcc atggagccca agggcacccg catcttcggg ccggtggcgc gcgagctgcg
     2581 cgagaaacag ttcatgaaaa tcgtctcttt agctcctgaa gtgctttaga atattgataa
     2641 aggcaatcga caatgcgtgt tagaaaaaac gaccaggtcc tggttattgc cgggaactac
     2701 aacgggaaaa agggcaaggt cctgaaggtt ttcccggaga aatcccaggt gatcattgag
     2761 ggcgtccact ttatcaaaaa gatgacgcgg ccgagccaga agaatccgca ggggggcatc
     2821 gtcgagaaag aggccccgat ccatgtctcc aatgtcatgg tgatctgccc caaatgcaac
     2881 accccggcgc gcatgggttc caagcaggtc ttcgatgaag ccaagagccg caagggccat
     2941 gtgcgcgtgt gcagaaattg taatgagatg ctggtaaccc agagttagcg tgaaccttaa
     3001 atcgggcata ataaaatggc tgaagaaaaa tataaaccac ggctgatcga cgtatatgcc
     3061 aaagaggtcc ggccgcagct gcagaaacgc ttcaactaca agaacgtcat gcaggtgccg
     3121 cgcatcgaaa agatcgtcct caatatgggc gtcggtgatg ccaccgagaa cgccaagaac
     3181 gttgatgtcg ccctggaaga gatgaccaag atcgccggcc agaagccggt catccgccgc
     3241 gccaagaagg ctatctccaa tttcaagctg cgcgcgggca tggcgatcgg ctgcagcgtc
     3301 accttgcgcc gctggcacat gtacgagttt ctggaccgtc tcatctccat cgccatcccc
     3361 cgcgtgcgcg atttccgcgg gctggcgctc aagtttgacg gccgtggcaa ctacaacatg
     3421 tcgatcaagg agcagatcgt cttccccgag atcgattacg acaaggtcga aaagacgcgc
     3481 ggcatgaata tcaccattgt aaccacggca aaaaccgatg aagaggcgct cgaactgctc
     3541 accgcgttcg ggatgccttt cattcgtcgt tcgtaatacc taaaatcaag aggaacggca
     3601 ttggctagaa aagcgttagt tgtcaagtcc aagagaaccc cgaaattcgc ggtgcgcgcg
     3661 tacaaccggt gttcccgctg cgggcggccc agggcctttt accgcaagtt cggcatctgc
     3721 cgactctgtt ttcgtgatct cgcgctcgaa gggcttatcc ccggcgtcaa gaaggccagt
     3781 tggtgaccct tccggagcct ttttgtaact aactttttca tcacagcgtt tagcttgtca
     3841 gggagaagat acatgtcaaa gactgatccc atcgcagact acctaacttg tatcaggaat
     3901 gcgaccaggg ccaatcatcc caaagtagat attcctgcct ccaggttgaa acaggagatc
     3961 accaagatcc tgcaggaaga agcctacata aaaaaccatg tcattatcaa tgatggcaag
     4021 cagggcgtca tccgcatcta cttgaaatat gacaatgaca acaggagcgt gatctccggc
     4081 ctgcgccgta tcagcaagcc cggctttcgc cgctacgtca accacaaggc ggtgccgcgc
     4141 gtcttcaaca atctgggtat tgccattgtc accacctcgg aaggggtgat gaccgggcag
     4201 gaagcgcgca aacgcggcgt gggcggcgaa gtactctgct atatttggta aatccttcaa
     4261 aacaggagta tatcgtgtca cgagtaggaa aagctcccat tagcataccg gatggcgtcc
     4321 aggttgacgt caccaacaac ctggtgacgg ccaccggtcc caagggcacc ctggcgtacc
     4381 aggtcgatcc gcgtctgatc gtcaagatgg agaacggcac cctggaggtg cagcgtccga
     4441 acgacgacaa gatcgtgcgc agcctgcatg gtctgacccg caccctggtc aacaacgtcg
     4501 tggagggggt ctcgaagggc tttgagaaga agctcgagat catcggcgtc ggtttccgcg
     4561 ccgagatgaa gggcacgcat ctgctgctct cgctgggcta ttcgcaccag atcatcgtcg
     4621 ctccgcccga ggggattacc ctggcgaccg aggggaacaa cattgtcgtg gtgcgcggca
     4681 atagcaagga gctggtcggg cagatcgcgg ccaagatccg cagcctgcgc aagccggaac
     4741 cctacaaggg caaaggcgtc cgttatctgg gcgagaccgt ccgcaagaag gccggcaaga
     4801 ccgcggccta atccggtggg ccgcgcggcg cactgacccg tttgtgggca gggagccgcg
     4861 atggactgga atgaaccata acctgatgag tttatacaat ggctgataaa tcatacacca
     4921 aagtggaagc gcggcatcgc cggcacagcc acattctcaa gaccctgcgc ggcagcgcgg
     4981 agcggccgcg gctggtgatt ttccgcagca acaagcacat ctatgcccag gtggtcgacg
     5041 atctgagccg caccaccctc ttcgccgcca gcactcttac ccccgcgatc cgcgatgtgg
     5101 tcgccaaggc gaagagcaag aaggaacagg cgatgctggt cggcaagcac ctggccgaga
     5161 tcgccaagga caaagaggtc acccggatcg tcttcgaccg cggcggctat ctctatcacg
     5221 gccgcgtcaa ggccctggcc gacggcgctc gcgaaggcgg cctggaattc taggcttgga
     5281 gcacatggat tttaacatga ggaacagacg ttggaaaaga taaatcccgg tgaatttgaa
     5341 ctgaccgagc gggtggtcca ggtcaaccgc gtggccaaag tggtcaaggg cggccgccgt
     5401 ttcagtttta atgcgatcgt cgtcgtcggc gacggccacg gccgcgtcgg aatcggcctg
     5461 ggcaaggcca atgaagtgac cgattcgatc accaagggcg ccgatgccgc caaaaaggcc
     5521 ctcatccgcg tgcccgtggt caacggcacc atcacccacg aaatcgaagg caaatacggc
     5581 gcgggccgcg tcttcctgaa acccgccgcc cccggcaccg gggtcatcgc cggcggcgcc
     5641 gtgcgcgcca tcctcgaatc cgccggctat accgatatcc tgaccaagag cagaggatcg
     5701 gccaatccgc acaacgtcgt caaggcgacc atagcggccc tcgaaaacct catggatgtc
     5761 gcaggcgtct cgcgcaaacg cggccttcag gtgatgcagg tttttaagac cgtgcccaag
     5821 ccgaaagttg gcgctaacga ataacaggtg gacaagaggt catgaacaag aagaaaaaag
     5881 aagccaagca gatcaagatc acgctggtgc gctccaccat cggatcgatc cagaagcaca
     5941 aggcgacagc catcgctctg ggcctgcgcc atcccaacaa cagcgtggtg cactatgaca
     6001 cgccgatcat ccgcggcatg gtcgacaata tccggcactt ggtcaaagtg gaagaactgt
     6061 aggtataacg caacaggctg aggatataat gaatttagca aatttgacat atgcggaagg
     6121 cgcggtcaag gaccgcaagc gggtcggccg cggcgggtcg cgcggcaagc agtccggacg
     6181 cggctccaac ggcgccggct cccgttccgg ctccaaacgg cgcgcctggt tccagggcgg
     6241 gcagatgccc atccagcgcc agctgcccaa acgcggcttt aaaaatctca atcgcatcga
     6301 gtttcaggtg gtcaatgtcg gcgtcctcga caaactgccc ggcggtgaag aactgactcc
     6361 cgccctcctc ttcgaattgg gcgccatcgg caagaaggat accccggtca aggtgctggg
     6421 cgatggcgaa gtgaccaaga agctcaccgt ttcggccaat gccttcagca agagcgcaca
     6481 ggaaaagagt gaggcggccg gagggaggat tacaaagata tgatgggcag cctgggcaac
     6541 atcttcaaga ttccggatct gaaacggcgc atcctcttca ccgccgcggt tttgatcgcc
     6601 taccgcatcg gcggccatgt tccccttccc ggcgtcaacg ccgcggccct ggtcaactat
     6661 ttcaagtcga ccgcctcggg cctgatgggg ctctatgacc tcttcgccgg cggggccttc
     6721 agccgcgcca ccgtcttcgc cctgggcatc atgccctaca tctcggcctc gatcatcatc
     6781 cagctgctcg gcgccgtcgt gccctacttc cagaaactgc cgaacaggga cgcaaaaaga
     6841 tcacccagct cacccgctac gggactgtgg ccatctccgc cctgcaggcc ttcggcgtca
     6901 gcatctttct ggagagcctc aacgcctctt cggccgccgc caccgagctg atcgtccccc
     6961 atccgggctt cggcttccgc ttcatcacca tgatgggcct gaccgccggc accgtgttga
     7021 tcatgtggct cggcgaactc atcactgaac gcggcatcgg caacggcatc tcgctgatca
     7081 tcttcatcgg catcgtcgcc cgcttcccga actcgatctt cggcgagttc cagcaggtgg
     7141 ccgccggcaa ccgcaccatc ctggtcgaga tcgtcctctg gctcctcttc gtcggcaccg
     7201 tcgccggcac cgtcctcctc acccagggga tgcgcaagat tccggtccag tacgccaaac
     7261 gcgtggtcgg ccgcaagatc tacggcgggc agagcaccca cattccgctc cgcgtcaaca
     7321 ccgcgggcgt catgccgatc atcttcgccc agtcgattct cttcatcccg cagaccatca
     7381 tccagttttt cccgaacagc gatttcatgg tctcgatgaa cagcctcttc agctacgatt
     7441 cgagctttta ctggctgatc tacggcatca tgatcgtctt tttcacctat ttttacaccg
     7501 ccatcgcctt caacccggtc gatgtggcgg acaacatgaa aaaatacggc ggcttcatcc
     7561 cgggcgtgcg tccgggcaaa aagacctccg aatatatcga tcacatcctg accaggatca
     7621 ccctgccggg ctcgattttt ctggcgatca tcgcgatctt tccatccttt gtcgccaagc
     7681 tgaccaatgt cggctatgat ttcgccagct ttttcggcgg cacctcgctg ctgatcgtgg
     7741 tcggtgtggc cctggatacc ctgcagcagg tcgaatccca cctgctcatg cgccactacg
     7801 acggttttct caagagcggc aagattcgcg gccgccgccg cttctagcgt gaaagggaat
     7861 ctgcagcgat gagagttatt tttctcggcg cccccggcac aggcaagggc acccaggcgg
     7921 tctggctgag cgacaagttc accatccccc agatctccac cggagacatc ctgcgcaagg
     7981 cggtggccga tggcaccgaa ctgggcaaaa aggccgacgt ggtcatgaag agcggcggcc
     8041 tggtctccga cgatctcatc atcgctctgg tgcgcgagcg cctcgaccag ccggactgcc
     8101 ggccgggctt tatcctcgac ggttttccgc gcaccatcgc ccagggcgag agcctcgacc
     8161 tcatgctggg ccgggacggg gcgatccaga gcgtgatctt ttttgaggtt tcggaagccg
     8221 agatcgtccg gcgtctcacc agccgccgca cctgcagcgg ctgcgcccgc aactataaca
     8281 tgatttccga tcccccgccg gcctccggcc tctgcggctc ctgcggcgga cggatcatcc
     8341 agcgccccga cgacagcgag gagacggtgc gcaaacggct gcaggtctat gatgaaaaga
     8401 ccgctccgct aaaagagttt tacaataaac aaaaaaagtt gtatattcta cgcgctgaac
     8461 agccggtgga gcgcgtccgt gaagagctgg ctgccctgct ctcgggactc taggccgtca
     8521 tgatcaatat ccgcagcgag aaagagatcg atctgatccg gcagagcggc cgcatcctgg
     8581 ccggcgccct gcacctggcg gaatcgagca tcgctgctgg agtggtcacc ggcgaactcg
     8641 accaggagat agccgcctac attcgcagcc ggggcggcaa gcccgctttc aaaggctaca
     8701 acggctaccc ggccaacacc tgcatctcca tcgacgagca ggtggtgcac ggcataccgg
     8761 gccaccgggc ccttcaggag ggggagatcg tcagtgtgga tgtgggtgtt cttttgaatg
     8821 ggtactacag cgatgcggcg cgcaccttca aggtgggcgc ggtcagcgcc gaaaaggaac
     8881 ggctgatgga ggtgaccctc gcctcgctgg gcaagggcat cgcggccgca gtcgacggca
     8941 accgcctctc cgacatcggt cacgctgttc agagccacgt cgaggccgcc ggtttttcgg
     9001 tggtgcgtga gctggtgggg cacggcatcg ggcaggccat gcacgaagag ccgcagatcc
     9061 ccaactacgg ggctccggat cgcggtcccc gtctcaggga gggcatggtc ttcgccatcg
     9121 agcccatggt caacgccggg gactggcagg tgaaaaccct cgaggaccag tggacggtcg
     9181 tcaccgctga tggcaagccc tccgcccatt atgaacacac cgtggtcatc cgcaagggcg
     9241 ccgcggaaat actaacactg gagtagactg atactgtatg ccaaaagaag cttccatcaa
     9301 aatcgatggt gtcatcctgg aaaccttacc caacgcctcc ttccgggtgg agctggagaa
     9361 cggccacaag gttctggccc acatctccgg aaagatgcgc atgcatttca taaaaatact
     9421 gccgggcgac aaggttacgg tcgagctttc gccctacgat ctgtcccgcg gacggatcac
     9481 ctatcgtttc aaatgagctg caccagaaca tccaattttt gaggcaagag atgaaagtca
     9541 gatcctcggt aaagaaaatt tgcgacagct gtaaaatcat acgccgcaag ggtacgattc
     9601 gcgtcatctg caagaagaat ccccggcata aacagcgtca gggatagggc atatttaaac
     9661 aaaggagatt gctttggctc gtatttcagg catagaccta ccgcgcgata aacgcattga
     9721 agtggccctc acctacatct acgggatcgg cctcaccact tcgcgtgaaa ttctcgaaaa
     9781 gacccggatc aatccggaca cgcgcgtgcg cgacatgcag ccggatgaag tggccaagat
     9841 ccgcgacctc atcgcgaacg actacaaggt ggaaggcgcc ctgcgcagtg aaacgcagat
     9901 gaacatcaag cgcctgatcg atatcggttg ttatcggggc atgcgtcatc gccgcaatct
     9961 gccggtgaga ggacagcgca cccataccaa tgcgcgcacg cgccgcgccc gccgcggtgg
    10021 tgtcggcatt cgcaagaaaa agtaatctgt tcggcaaacg gcttttattc tagagaggtg
    10081 tagattggcc aaccccaaaa agaagaccac caagaaacgc gaaaaggtgg aggcgaacgg
    10141 tgtcgcccat atccgggtga ccttcaacaa taccaacatc acccttactg acatgtacgg
    10201 gaatgtcatc agctgggcca cggccgggcg ttccggcttc aagggctcgc gcaagagcac
    10261 tccctacgcc gcccagatgg ccgctgaatc ggccgccaag gaggccatcg acctcggctt
    10321 gcgccgcgtc gaggtgctgg tcaagggccc cggttcagga cgcgaagcgg cagtccgctc
    10381 gctccaggcc gccgggctgg agatcagctc gatcaaggat acctcgccga tcccgcacaa
    10441 cggctgccgt ccgcccaagc gccgccgcgt ctgatcgacg tccccgcacc ggggttttca
    10501 agtaattcta tcggttcgtc gcacacgata tgccggtccg tcaaacggac ttttacgagg
    10561 aggttccatt ttatggcaag agacactgga gcagattgca aactttgccg ccgcgaaggc
    10621 atgaagctct ttttgaaggg caccaaatgc accagcgata aatgcccttt tgacaagaaa
    10681 ggatatgctc cgggacagca cggccgcacc cgccgcttca agcagtccga atactccctg
    10741 cagctgcgcg aaaagcagaa ggtgaaacgg atctacggcc tgctcgagag ccagttccac
    10801 acctattttg aacgcgccga gcgcctcagg ggaatcaccg gcgagaatct gctccgcctg
    10861 ctcgagaccc gtctcgacaa cgtggtctac cgcctcggcc tcgccccctc gcgcacgacc
    10921 gcgcggcagc tggtgcgcca ccgccacgtt ctcgtcaacg gcgaactggt cgatatcccc
    10981 tcctaccagc tcaaggccgg cgacgagatc cgcatccgcg aaaagagccg ccagctcgag
    11041 atcatccaca gcgccatgaa gcgcgtgcgc gacggcaaga tggtcgcctg gctctccctc
    11101 gacaaggcgg gcatgctcgg caccattctc gaacagccgg cgcgggatgc catccccgtg
    11161 gatgtcaacg agaaactgat cgtcgagctc tattccaagt agtccggacc gaggcgttcc
    11221 ggaacagctg ttgtcaacct catcattgat caatggagct ttaaatggct ttaccaagtt
    11281 ttcagatgcc cgaaagcatc gaactggatg agtcaacata taccaatacc tacggaaagt
    11341 tcatcgtcca gcccctggag cgcggcttcg gcgcgaccat cggcaacgcc ttccgcaggg
    11401 tcctgctctc gtcgatcccc ggcgccgcca tcaccacctt ccgcgtcgac ggtgtcctgc
    11461 acgagttcag caccatcccc ggagtcaagg aggatgtctc caccatcgtc ctcaacctca
    11521 agcaggtgcg tctcaagctg atcgataaaa agccggacaa gatctcgatc cacctcaagg
    11581 ggccgaaaac cttcaccgcc cgcgatatcc aggagcagag caccgatttc gaggtcctca
    11641 atcccgactg gcatatcgcc accctcaacg ccgatgccga tttcaccatc gaactgcgca
    11701 tcggccgcgg ccgcggctac gtcccggccg aggagaaccg gctgcccgat ctgcccctgg
    11761 ggaccatccc catcgatgcg gtctttaccc cgatcgtacg cgtcacctat acggtcgaaa
    11821 acacccgcgt cggccagcgc atcgactatg agcggttgat cctcgagatc tccaccgacg
    11881 gcagcatcac ccccgacgac gccctcagct tcgccggcaa gatcctgcgc gaccacatcc
    11941 agctcttcat caatttcgat atcgacaccg aggaggagga atcgcaggag gtagatgagg
    12001 agtcgatccg catccgcaag ctcctcaaga tgagtgtcga cgaactcgaa ctttcggtcc
    12061 gttcgcacaa ctgcctgatg gccgcgaaca tcaagaccat cggcgacctg gtccgccgcg
    12121 atgagcagga gatgctcaag ttccgcaact tcggccgcaa atccctctcc gaactcgcca
    12181 agatcctcga ggaaaagggg atcaccttcg ggatggatgt ggacaaatat ctgagaaaag
    12241 gcgacgacta agagagcagc catccccgca tggctttgaa tctgcaagag tttgttgtac
    12301 cctattgaag gattctattc atgagacacg gcaaatctgg aagaactttg ggcaagaccc
    12361 acagccatcg caaggcgatg atgaacaata tggcggcctc gatcatcagc cacaagagca
    12421 tcatcaccac cacccccaag gccaaggaag cccggggggt tgtcgaacgg ctgatcacct
    12481 tcgccaagaa gaatgatctc gccgcccggc gcatggtgat gcgctcgatc aaggacaagg
    12541 agctggtccg ggagctgttc gaggagatcg gccccaagta tgccgaccgc aacggcggct
    12601 atacccgcgt cgtcaagctt ggcaaccgcc agggcgacgg cgcctccatg tccatcttcg
    12661 agctggtcgg ctacgagggc gccaaggccg ccaagatcga gaaacagcgg cagaaacgcc
    12721 aggaaaaggc ggccaagaag aagaaagaga ttgaagaagc ggctcgccag gaacagcagc
    12781 agccgccgca ggaagaggaa ggcggcgaga agaagtagcc atcccctctt accaggtacc
    12841 tcccagggca atcgcgatga ttgccctttt ttttgcgcag gggccccggc aaggccttcg
    12901 ttccggcgcg gccggatgaa cgcaccagcc cggcgcaatg cggctgcgcg ggagcgatta
    12961 tttttgcttt cattttactc cgcttgttcg tacattatcc atattttccg cggaaccctg
    13021 cgcccctgcg gcggtttaat ccctgtccgc gcctcttcgc ctccaaatcc ggcgcgggct
    13081 ccgcaccata atcgggaaac cacatgaaaa gacttttttt gcccgcagcc cttctgcttc
    13141 tcgttctggt cctggcttgc accaacgaag agcccctgcc gagcggcctc gacgccctcc
    13201 atcgcgccaa caccgattcc ctccgcgtcg tcaccatccc ggcgcaggag gcggcctggt
    13261 tccgccgtga ggtgaatccc ggcctcaccg ccagcctgct ggtcggacgc catgacaaca
    13321 tcaccgccag ggccctgctc aaattcaccg gcttttccac gatcgatacc gcctcggtca
    13381 acgctgcaac cctgaccctc acccagaagg ggcgctgggg agcgggcggc gcctttacct
    13441 gcacggtcca caaggtcacg gccgcctggg cggaaaaatc ggtgcagtgg agtgaaatca
    13501 gcggcggcta tgagcttgcc ccgctcgcct ccttcgagat cctggcccag gattcggcca
    13561 cctatgcggt cgaactccct cccgccgtgg tcaacgactg gatcggcggc tccgacaaaa
    13621 acgggctgct cctcgatttt accggcgccg acatggcggc ggagctgggt tccgtcgacg
    13681 tctccagcgg gggctatccg accctgaaac tggccttcac gaccaaaaaa ggggaggccg
    13741 atacggtcac ggtcagcccc gccgaggacg ccaccctctt cacctgggat caacttccgc
    13801 cggtggatgt ggtcgagagg gg
//
LOCUS       DAOPWU010000054        13808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4529_length_13808_cov_4.845925, whole genome shotgun sequence.
ACCESSION   DAOPWU010000054 DAOPWU010000000
VERSION     DAOPWU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4529_length_13808_cov_4.845925"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..804)
                     /locus_tag="PKM23_04685"
     CDS             complement(106..804)
                     /locus_tag="PKM23_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY90801.1"
                     /translation="MSIASTGSHLFAGTYGGLFLSLDEGLQWHQIDPTGANPMPRTGE
                     KENPGSLSREPKTVYTLAVRGRHVFIGMMENGVYFTDDGGQSWIPLNEGLSRLSLYSI
                     FTSPFHLFVGKYGGGVGRYPLSGIISSIRLPGRAASRSIQLRPNYPNPFNPSTTITFE
                     IPDLGGAMSALNNVRLTVYDPAGREVVQLLEGWRGPGTHSVQWDARGLPAGLYLCRLS
                     AGSQVQSRKMLLVR"
     gene            complement(942..1397)
                     /locus_tag="PKM23_04690"
     CDS             complement(942..1397)
                     /locus_tag="PKM23_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90802.1"
                     /translation="MKPTATAILTYLFIATATIRVSAQWFETTAPEGGTVNSFAWSSG
                     RLYAGNNDGVFLSSDSGATWMALNEGLTDRRIWSVAVHNGMLFAGTNDSGAFRSWDDG
                     FTWVRMKHGLGDRRIRALASDGMNLFAGTLGGSSARTTTASPGGPSIRG"
     gene            complement(1484..2896)
                     /locus_tag="PKM23_04695"
     CDS             complement(1484..2896)
                     /locus_tag="PKM23_04695"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNY90803.1"
                     /translation="MRRILAILALVAALAAGSTAAEKKRILQADYATFKYDSSQTYWE
                     FYYSVYRKGVTYQQSEQGDFSSQIIMRLVLNRGDSLWAQKAWKFVDTVADTHAANMDA
                     VMVDHFYLLVPAGSYSGILTAQDLNNPALIDTVLWDEQIDLYGSRGPALSEIELASTI
                     SQAGPEESSPFVKNRLVVIPNPSQLFDEKADQLFFYVESYNMYSAFPDPFYRIRYYIS
                     DLKGVPVAGSLPVDLKRKASGDRRIEFGALGIGSLLSGSYLLNFDLLDTSHTVLAHRE
                     KQFYLYKPQPVFAGGAALDQDQLFLTSDFAVMDEKQIEQVFEYSSYLLLKEQRDAFRD
                     LGDLNARRRYLFSLWRERDTSPETAVNEYYLAYMERVRFANKNYSRPKREGWHTDRGR
                     ILLLFGPPTSVDSYPNELEVKPYEIWNYDDLEGGVIFVFGDLFGINDLQLLHSTKRGE
                     IRDENYLQKLGKKADSDLFR"
     gene            complement(2971..4020)
                     /locus_tag="PKM23_04700"
     CDS             complement(2971..4020)
                     /locus_tag="PKM23_04700"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY90804.1"
                     /translation="MKFKYSVLFVLSLFLTIGLVLQAEAGTSKRTGTNAADELLIPVG
                     ARGTALGGANLSTVIGTEAIFYNPAGLGMMDGAAEVLFSHQQYLADIGVNYAAIGART
                     GVGNIGLSLKSLSFGDIPVTTEDAPEGTGATFSPNFMTVGISFARSMTDRITAGINLK
                     LINESIMSTNANALAFDLGVQYRTGMGVNIGVSMKNIGSSIKYSGQNLEKLVGVPGSE
                     PTAADRRMAIPAEKAELPSVFEIGLSYDYVPVEKVNMSFMGNFRNHNFQNDEVLAGAE
                     FDYAKTVFLRGSYSYQLNSEEDVQGGLSYIYGPAFGFGLAYPLTATTKICFDYAYRMA
                     EYFDANQLFTLTLTF"
     gene            complement(4050..7103)
                     /locus_tag="PKM23_04705"
     CDS             complement(4050..7103)
                     /locus_tag="PKM23_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90805.1"
                     /translation="MIKKSLTLFLTLAVFFTLALNPLWAKAEKGAPRLGKSMDTRHAE
                     TFDVNRIDSWFINNGLFASDPVTEDAGLIYPKGSGHYAIFTAGLWLLGITEDTKELRA
                     CAADYSTDMQPGRILDSGAPDDPTKSEYIVYKLNKGESVPAAAIAQGAPAEVLGDQML
                     FCVYNDANPTLHAEVWMTQPIGVEIRQTVWGYAQAGPLGDACFMRFEFVNKGLSNLDS
                     TYVALFFDPDLGDATDDYVACDTDLSLGFVYNGDAYDQEYGSSIPAIGCDFFQGPLAD
                     SPGDTAYLPSGVFPDKKVLPMTAFVKYINSNATYSDPDLQSANGGFQAYNYVQGFIWD
                     GSEFIDPFTGLPSKFVNPGDPVTGTGWLGFYEAPPADHRMVIASGPFKLVKGQPMEVV
                     AGLVAGMGNNNLNSVSIMKYNDKVAQFAYDNNFVVGNPPPQPVVTVFEGDQEIVLTWD
                     ETARDYADAAGYAFEGYNVYQAPAASGDAQGKWKRIATFDKANYITKVYDEAFDNESD
                     AIVTKPVQFGTDNGLAYQMILEKDYYAGGTPLVNGKKYYFAVTGYTVNLTGTPKALEN
                     VITAITCVPHKPEMKTELRAETASALTTTIVPGDGASAGDISATVKVVDPAAVTGHDY
                     KVEFNYLDSGDYAGFADSWKLVDVTTGKVLINSWDTIGGGTDYPVVDGLEVKVIIPAP
                     GQYGLNMNKGTYAANGAGYAGVTIVNNANRTLTGVNSGMESFFKGGGIGENFLGSTLT
                     ALDYVDVRLVFTPDESKWSKAYVYHNPGGYAYMGVGTFPGYAEDISNPAAPRRLNIAF
                     RESPGASNAVDMHWDPNAADFGDGLGGREYLFIMKSTYDDGAGLYYGEGKPSARTADV
                     LYALWAHNRGSYTSVVEKGEWGITWLCYHPVKAGDHVTFTTAGMNKVASDEVGVKNID
                     KISVFPNPYFGVNSAELANFEQFVSFINLPTDECTIRVFTLSGQLIRTIVHDNGTSMD
                     KWELKNDDSVPIGSGVFIVHIDVPNVGQKILKLAVVNREARYSHF"
     gene            complement(7163..10081)
                     /locus_tag="PKM23_04710"
     CDS             complement(7163..10081)
                     /locus_tag="PKM23_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY90806.1"
                     /translation="MLDKETGEALPGANVLVVGTQMGATSDVNGNFMILNVPPGTYVL
                     KATFVGYTQVSMSNVKVIPDVTTESNFKLPQVALEGETITIVAEKPLVDKNVTNYTKT
                     VWAEDLANLPVRTVQNVVATLAGAVQQGGNLYVRGGRSHETARYVEGMMTSSIVDGGE
                     NISIVDRSIELIDFQAGGFTAEYGFANSGVVHTTIKTGGPSYKVSLEAVSDDFWAVDG
                     KEKGYQILGLNKLYSYGYDDYFATLSGPVIPGQKNLRFFVAGQHSYRLSNATRFSGWT
                     SPEYLVPGSWRTTEGTTVYDTLNLAANIAPGRIPGGGNQFDVANGNVVWDLGALRFKV
                     GGSYTWQRSQDRPATVPLNATNTIYNTEASKRWRQTNYSTYLQMTHNVNPTMFYTWNV
                     NYFADNAESGHPVYWQDINKWGDPLYNKAYSDTGTVKTYSFMDLTAYDPAVGTSGTIT
                     KASESFIGTKLDLTKQFGRQHEVKVGGEFNYYTIRRYSLAARSMLTSLTNQARSMGTV
                     NEITDYYAYRSFLTNYGYDIYGNEISEDQTYTANVAGEIIPFSGHDGPPHPVLAGAYI
                     QDKIELKDMVLNLGVRMDYFDRGINKLRNWETLTQSSSGFFDEEVFVESDPDIYISPR
                     IGFSFPVTDMTVFHAQYGKFVQMNRFDQLFGSWGTTAFNLFASGYARTMPNPNLKPER
                     TTSYEFGFRQQLGQSAAFDATLFYKNTDDLIAIRKIRPAEGSVYASPYLFSNADFGTI
                     KGVSFDFQLRRWKRIAASANYTYSFSEGTGSAADSHFDIAWAEGSPVFPVTIAPLDFD
                     IRHKGTISADIRMGAEDGPALFGLKPLANVGLNMLFTFNSGAPYTRVEAGGGYSEVHG
                     YTQALPIEALNQSRMGWFYNIDAKLDKTVKVAGIDFNLYLWALNLLDTKSVTAVFLQT
                     GTNTSDGWLDSAAGRQAQANSTLGAEEYARWYNAQLTSMGTFGWSAPRQLRAGIKIDL
                     "
     gene            complement(10755..11342)
                     /gene="yihA"
                     /locus_tag="PKM23_04715"
     CDS             complement(10755..11342)
                     /gene="yihA"
                     /locus_tag="PKM23_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNY90807.1"
                     /translation="MKILSAEYIKSVARLEDLPRDRLPQIAFAGRSNVGKSTLLNVLF
                     NRRNLAYVSSTPGKTQTLNFYRVNRQFYFVDLPGYGYAKSPKSLRQQWQGLIESYLTG
                     VEELRLVVVLVDVRLSLQELDAQLLEWLRLQGRSCIVVATKADKLSGSELQKNLAGLR
                     AALGEGDFGKIVPFSAKSGRGKEEVWRLLQGALER"
     gene            complement(11339..12613)
                     /gene="clpX"
                     /locus_tag="PKM23_04720"
     CDS             complement(11339..12613)
                     /gene="clpX"
                     /locus_tag="PKM23_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNY90808.1"
                     /translation="MDQKKQNGGRRLVICSFCGKSSQQVETIVTGPDVTICSECVTIA
                     NKIIAREKSRRTTATVERVPTPAEIKAELDKFVIGQEHAKQTVAVAVYNHYKRVNHAA
                     PADEVMLEKSNVLLIGPTGTGKTLIAQTLARLLQVPFTIADATTLTEAGYVGEDVENI
                     LVRLLQAADYNLERAERGIVYIDELDKIARKNGNPSITRDVSGEGVQQALLKILEGTI
                     AAVPPKGGRKHPEMEFININTANILFICGGAFESLEKLIASRLNKMAIGFGAESVKGD
                     ALELGELLRRVEPEDLLQYGLIPELIGRLPIICTLEELDDEALLRILTEPRNALTRQY
                     QRLLALDGVELEFDRAALQEIVRIARKRGTGARGLRSVLEETMLRIMYELPSRKEISH
                     CRITEAVVTSGAEPRYKKRKLPPENPVQESAA"
     gene            complement(12638..13243)
                     /gene="clpP"
                     /locus_tag="PKM23_04725"
     CDS             complement(12638..13243)
                     /gene="clpP"
                     /locus_tag="PKM23_04725"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNY90809.1"
                     /translation="MGLVPIVVEQTGRGERAYDIFSRLLKERIVFLGSAIDDNVASLA
                     IAQLLFLAAEDPAKDVYLYINSPGGIVSSGLAIYDTMQYIKPDVSTICIGQASSMGAL
                     LLAAGAKGKRFALPHSRVMIHQPMGGAQGQATDIEIQTREILVIREKLNQILVHHTGQ
                     PMERIVKDTDRNFFMSAEEAKEYGLIDEIMVRKDLVKPKKK"
     gene            complement(13254..>13808)
                     /locus_tag="PKM23_04730"
     CDS             complement(13254..>13808)
                     /locus_tag="PKM23_04730"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90810.1"
                     /translation="EFAKDLGAFSSLEELKADIRARLQAQAEHQTHERFRDALADELI
                     KQTPIEAPPSMVDNYLAMLIADVKRKSKESVDEAHLAEHYRPSAVHGVKWYLIRDRLI
                     SQQGLAVSDEELESALSAMAAAGEEGAQRAEQIRSKKEERERFRDALEDDKVYAFLSQ
                     KAKVTEVRRSLYQHHDHEHPSAQA"
BASE COUNT         2723 a         3731 c         4445 g         2909 t
ORIGIN      
        1 atttttttaa ttaattatct ttgggtggga acgagatttc ggggggtagc ctgttaatta
       61 tgccaggcgg gcgagggagc gcggcacttt cctctttaag gggctttagc gcaccaggag
      121 cattttcctg ctctgcacct ggctgccggc gctgagacgg cagagataaa ggcctgccgg
      181 caggccccgg gcatcccatt ggactgaatg ggtgccaggc ccgcgccagc cctccagcag
      241 ctggaccacc tcacggccgg cgggatcgta aaccgtgagg cggacattgt tcagagcgga
      301 catggctccc ccgaggtccg ggatctcgaa agtgatggtg gtactcggat tgaagggatt
      361 gggataattc ggcctcagct ggatggaacg cgacgccgcc cgcccgggga ggcggataga
      421 gctgataatc ccgctcagag ggtagcgccc cactcctccg ccgtatttgc cgacaaaaag
      481 gtggaagggc gaagtgaaaa tggaatagag ggagagccgg ctcaatccct cattcaaggg
      541 gatccagctc tggccgccgt cgtccgtgaa atagacccca ttctccatca tgccgatgaa
      601 aacatggcgc ccccgcacgg cgagggtgta gaccgtcttg ggctcccggg aaagggaacc
      661 aggattctcc ttctcacccg tccgcggcat gggattggcg ccagtgggat cgatctggtg
      721 ccactgcagc ccctcgtcca gggaaagaaa gagtccgccg taggtccccg caaagagatg
      781 agatccggtc gaggcgatgg acatgatatt gacattctcg agtccagcat tgcgctgctc
      841 ccaggtgctg ccgctgtcgc tggagaaata gatgccgccc cccagggttc cggcccagat
      901 cctgccttcg cagcagtgga gggtgcggac gaacagattg gtcagcccct gattgacggc
      961 ccgccaggag aggccgttgt cgtccgagcg gaagaccccc ccagggtgcc ggcgaagagg
     1021 ttcataccgt cgctggcgag agcgcggatg cgccggtcgc ccagtccgtg cttcatgcgc
     1081 acccaggtga agccgtcatc ccagctgcga aaggccccgc tgtcgttggt gccggcgaag
     1141 agcattccgt tatgcacggc gaccgaccag atgcgccggt ccgtcagtcc ctcattgagg
     1201 gccatccagg tggcgccgga atcggaggag aggaagacgc cgtcattgtt gccggcgtag
     1261 agcctgccgg aggaccaggc gaaggagttg acggtacccc cctctggggc ggtggtttcg
     1321 aaccattgcg ccgaaacccg gatagtggcg gtggcgatga acagatacgt cagaatggcg
     1381 gtcgcagtcg gtttcacgag agaacccctc catttccatc ccgaaaataa aaatgcttcc
     1441 ggataaagtc cattccggaa gcaagtcagg accacagcgg cgatcagcgg aagagatcgg
     1501 aatcggcctt tttgcccagt ttctgcaagt aattttcgtc tctgatctct ccccgtttgg
     1561 tggagtggag cagctgcaaa tcgttgatcc cgaagagatc gccgaagaca aagatgaccc
     1621 cgccctcgag atcgtcatag ttccagattt cgtagggctt gacctcgagt tcattggggt
     1681 agctgtccac cgaggtgggg ggaccgaaga ggagcaggat gcggccgcgg tcggtgtgcc
     1741 atccctcccg cttgggacgg ctgtaattct tgttggcgaa acgaaccctc tccatgtagg
     1801 cgagataata ttcattgacc gcggtttcgg gcgaggtgtc gcgctcccgc cagagtgaaa
     1861 aaaggtagcg ccggcgtgca ttgagatcac cgagatcccg gaaggcatcg cgctgctcct
     1921 tgagcaaaag ataggagctg tattcaaaaa cctgctcaat ctgtttttca tccatgaccg
     1981 cgaaatcgct ggtcaggaag agctgatcct gatcaagggc ggcgcctccg gcgaaaacgg
     2041 gctgcggctt gtagagatag aactgcttct cgcggtgcgc cagaacggtg tggctggtat
     2101 cgagcaaatc gaaattgagc aagtagctgc cgctcagcag gctgccaatg ccaagggcgc
     2161 cgaattcgat gcgccggtca ccggaggcct tgcgcttgag atcgaccggc aagctgccgg
     2221 caacgggcac ccccttgaga tccgagatat aatagcggat gcggtaaaag ggatcgggaa
     2281 aggcggagta catgttgtag ctctcgacgt aaaagaagag ctgatcggcc ttttcatcaa
     2341 agagctggga agggttgggg atgacgacca gcctgttttt cacgaagggt gagctctcct
     2401 cgggcccggc ctggctgatc gtgctggcca gttcgatttc gctcagggcc ggcccgcgcg
     2461 agccgtagag atcgatctgc tcatcccaaa gcacggtatc gatgagggcg ggattgttga
     2521 gatcctgcgc cgtcaggatg ccggagtagc taccggcggg aaccagcagg tagaaatggt
     2581 ccaccatgac cgcatccatg ttggccgcat gggtgtcggc tacggtatcg acgaatttcc
     2641 aggccttttg tgcccaaagg gagtcaccgc ggttcaggac gagccgcatg atgatctggc
     2701 tgctgaaatc tccctgttcg ctctgctggt aggtcacacc cttccggtag acggaatagt
     2761 agaactccca gtaggtctgg gaagagtcat atttgaaggt ggcataatcg gcctggagaa
     2821 tgcgcttctt ctccgctgcg gtggaccccg cagccagggc ggccaccagg gcaaggatgg
     2881 caaggattct tctcatggcg gtcccatggt taaaggataa gaaaaaaggg atggcccttg
     2941 cgggccatcc cttgtggggc atttgcttct ttagaaagtg agcgtcagcg tgaagagctg
     3001 attggcgtca aaatactcgg ccatacgata ggcatagtcg aagcagatct tggtcgtggc
     3061 ggtgagggga taggccagac cgaagccgaa ggcggggccg tagatgtagc tgaggccgcc
     3121 ctggacatcc tcttccgagt tcagctggta gctgtagctg ccgcggagga agacggtctt
     3181 ggcataatcg aactcggcgc cggccaggac ttcgtcgttc tggaagttat ggttgcggaa
     3241 attgcccatg aacgacatgt tgaccttttc gacgggaacg taatcgtagg agaggccgat
     3301 ctcgaagacc gagggcagct cggctttttc agccgggatg gccatgcggc ggtcggccgc
     3361 ggtcggttcg gaaccgggga cgccgaccag tttctcgagg ttctggccgc tgtatttgat
     3421 cgaggagccg atgttcttca tcgagacgcc gatgttgacg cccatgccgg tgcggtactg
     3481 cacgccgaga tcgaaggcga gggcgttggc gttggtgctc atgatcgact cattgatcag
     3541 tttgaggttg atgccggccg tgatgcggtc ggtcatggaa cgggcgaacg agatgccgac
     3601 agtcataaag ttcggcgaga aggtggcgcc ggttccttcc ggagcatcct cggtggtgac
     3661 ggggatatcg ccaaagctca acgacttgag gctgaggccg atgttgccga cgccggtccg
     3721 ggcgccgatg gcggcatagt tgacgccgat gtcagccagg tattgctggt gcgaaaagag
     3781 cacctccgca gcaccgtcca tcatgccgag gccggcagga ttgtagaaaa tggcctccgt
     3841 gccaatgact gtggagagat tggcgcctcc gagagcagtg cctctcgcac cgaccgggat
     3901 cagcagttca tcagcggcat tcgtgccggt acgcttggag gtaccggctt ctgcctgaag
     3961 aactagcccg atggtcaaaa acaggctgag aacgaacaga acagaatatt taaatttcat
     4021 attctctttc tcctagttat caattgagac taaaagtgcg agtatctggc ttcccggttc
     4081 acgaccgcaa gcttgaggat tttttgtccg acattgggga catcgatatg gacgatgaag
     4141 acgccgctgc cgatgggcac cgaatcgtcg ttcttgagtt cccacttgtc catggaggtg
     4201 ccgttgtcgt ggacaatagt cctgatcagc tgaccagaga gtgtgaacac gcgaatcgtg
     4261 cactcatcag tcggcagatt gatgaaagag acgaattgct caaagttcgc gagctcagcc
     4321 gaattgacgc cgaagtaggg gttcgggaag acgctgattt tgtcgatgtt tttcacgccc
     4381 acttcgtcgc tggcgacctt gttcatgccg gccgtggtga aggtgacatg atcgccagcc
     4441 ttgaccgggt ggtagcagag ccaggtgatg ccccactcac ccttctcgac cacgctggtg
     4501 taggagccgc ggttgtgggc ccagagggca tagaggacgt cggcggtacg ggcgctgggt
     4561 ttaccctcgc cgtagtagag gccggccccg tcatcatagg tgctcttcat gatgaagagg
     4621 tactcgcggc cgccgaggcc gtcgccgaaa tcagcggcgt tgggatccca gtgcatatcc
     4681 acggcgttgg aggcgccggg ggattcacgg aaggcgatat tgaggcggcg cggagcggcc
     4741 ggattgctga tgtcttcagc atagcccggg aaggtgccga cgcccatgta ggcgtagccg
     4801 cccggattgt ggtagacata agccttgctc cacttgctct catccggggt gaagaccagc
     4861 cggacgtcga catagtcgag cgcagtcagg gtcgaaccga ggaaattctc gccgatgccg
     4921 ccgcccttga agaaagactc catgccgctg ttgacaccgg tgagggtgcg attggcattg
     4981 ttgacgatgg tgacaccggc atagccggcg ccgttggcag cataggtgcc tttgttcatg
     5041 ttgaggccgt actggccggg cgccggaatg atgaccttga cctcgaggcc gtcgacgacc
     5101 ggatagtcgg tgccgccgcc gatggtatcc caggagttga tcagcacctt accggtggtg
     5161 acatcgacca gcttccagct gtcggcgaaa ccggcgtaat caccgctgtc cagatagttg
     5221 aattcaacct tgtagtcgtg gcccgtcact gcggcgggat cgacgacctt gaccgtagcg
     5281 ctgatatcgc cggcgctggc gccatcgccg ggaacgatgg tggtggtgag agccgacgca
     5341 gtctcggcgc gcagctcggt cttcatctcg ggcttgtggg gtacgcaggt gatggccgtg
     5401 atgacatttt caagggcctt tggggtgccg gtgaggttga cggtgtagcc ggtgacggcg
     5461 aagtaatatt tcttgccgtt gaccaggggg gtgccgccgg cgtaatagtc cttctccaga
     5521 atcatctgat aggccaggcc gttatccgtg ccgaactgga ccggcttggt cacgatggca
     5581 tccgactcgt tgtcgaaggc ttcatcataa accttggtga tatagttggc cttgtcgaaa
     5641 gtggcgatgc gtttccactt gccctgggca tcaccggagg cagcaggcgc ctgatagaca
     5701 ttatagcctt caaaggcgta gccggctgca tcagcatagt cgcgggcggt ctcgtcccag
     5761 gtcagcacga tctcctgatc gccctcgaaa accgtcacca ccggctgggg aggaggattg
     5821 ccaacgacaa agttgttgtc ataggcgaac tgggcgacct tgtcgttgta cttcatgatc
     5881 gagactgagt tgaggttgtt gttgcccatg ccggcgacca gaccggcgac cacttccatg
     5941 ggctggccct tgacaagctt gaagggaccg gaggcgatga ccatacgatg gtcagcggga
     6001 ggcgcctcgt agaaacccag ccagcccgta ccggtgaccg gatcgccggg attgacgaac
     6061 ttggagggaa ggccggtgaa gggatcgatg aactcggagc cgtcccagat aaagccctga
     6121 acatagttgt aggcctggaa gccaccgttg gcactctgca ggtcgggatc cgagtaggtg
     6181 gcgttgctgt tgatgtactt gacgaaggcg gtcatcggca ggaccttctt gtccgggaag
     6241 acgccgctgg gcagataggc ggtgtcgcca ggggagtcgg cgagcgggcc ctggaaaaag
     6301 tcgcagccga tggccgggat ggagctgccg tattcctgat cgtaggcatc gccgttgtag
     6361 acaaaaccga ggctcaggtc ggtatcgcaa gcgacatagt catcggtggc atcaccgaga
     6421 tcaggatcaa aaaagagggc cacgtaggtc gaatcgagat tggagaggcc cttgttgacg
     6481 aactcgaagc gcatgaagca ggcgtcgcca agaggaccgg cctgggcgta gccccagacg
     6541 gtctgacgga tctccacgcc gatgggctgg gtcatccaga cctcggcatg gagggtgggg
     6601 ttggcatcgt tgtagacgca gaagagcatc tggtcgccaa ggacctcggc gggagcaccc
     6661 tgtgcgatgg ccgcagccgg cacggattcg cccttgttga gcttgtagac gatgtattcg
     6721 gatttggtcg gatcatcggg tgcaccgctg tcgaggatgc ggcccggctg catgtcggtg
     6781 gaatagtcgg cagcgcaggc gcgcagctct ttggtgtcct cggtgatgcc gaggagccaa
     6841 aggccggccg tgaagatggc atagtggccg gagcccttgg ggtagatcag gcccgcatct
     6901 tcggtcacag gatcgctagc gaagaggccg ttattgatga accagctgtc gatccggttg
     6961 acatcgaagg tttcggcatg tctggtatcc atgctcttgc ccaaccgcgg cgcccctttt
     7021 tcagccttgg cccagagggg attgagggcc agggtgaaaa agacggccag cgtcaagaag
     7081 agagtcaggc tcttttttat catattgaaa ttctccctat cattgtaatt tttgggtaga
     7141 acgatcctat cctggttcct tcttacagat cgatcttgat gcctgcgcgg agctgacgcg
     7201 gcgccgacca gccaaaggtg cccatagagg tcagctgggc attgtaccag cgggcatact
     7261 cctcagcgcc cagggtggaa ttggcctgag cctgccggcc ggccgcgctg tcaagccagc
     7321 cgtcggaggt gttggtgccg gtctgcagaa aaactgcggt gaccgacttg gtgtcgagca
     7381 ggttgagcgc ccacaggtag aggttgaaat cgatgccggc gaccttgacg gtcttgtcca
     7441 gcttggcgtc gatgttgtag aaccagccca tgcgcgactg gttgagggcc tcgatcggca
     7501 gggcctgggt gtaaccatgg acctcgctgt agccgccgcc cgcttcgacg cgggtgtagg
     7561 gagcgccgga attgaaggtg aagagcatgt tgaggccgac attggccagg ggcttcagac
     7621 cgaagagagc cggtccatcc tcggcgccca tgcggatatc ggcgctgatg gtgcccttgt
     7681 gacggatgtc gaaatccagc ggcgcaatcg tgaccgggaa gaccgggctg ccttcagccc
     7741 aggcgatgtc gaaatggctg tcggccgctg aaccggtgcc ttcagagaag gagtaggtat
     7801 agttggcgct ggccgcgatg cgtttccagc ggcgcagctg aaaatcaaag ctgacaccct
     7861 tgatcgtgcc gaaatccgca ttcgagaaga ggtacggcga ggcgtaaacc gaaccctcgg
     7921 cgggtctgat cttgcggatg gcaatcagat catcggtgtt cttgtagaaa agggtggcgt
     7981 cgaaggccgc cgactggccc agctgctgac ggaaaccgaa ctcgtaggag gtggtgcgct
     8041 ccggcttgag attcgggttc ggcatggtgc gggcgtaacc ggaggcgaag aggttgaagg
     8101 cggtcgtgcc ccagctgccg aagagctggt cgaagcggtt catctggaca aacttgccat
     8161 actgggcatg gaagaccgtc atgtccgtga ccgggaagga gaagccgatg cgcgggctga
     8221 tgtagatgtc cggatccgat tcaacgaaaa cctcttcatc gaaaaagccg ctgctggact
     8281 gggtaagggt ttcccagttc ctcagcttgt tgatgccgcg atcgaaataa tccatgcgca
     8341 cgccaaggtt gaggaccatg tccttgagct cgatcttgtc ctggatatag gcgccggcga
     8401 ggacgggatg gggcggacca tcatggccgc tgaaggggat gatctcgccc gctacgttgg
     8461 ccgtgtaggt ctggtcttcg ctgatctcgt tgccatagat atcataaccg tagttggtca
     8521 ggaaggagcg ataggcgtaa tagtcggtga tctcattgac cgtgcccatc gacctggcct
     8581 ggttggtcag cgatgtgagc atgctgcggg cggccagcga gtaacggcga atggtgtaat
     8641 agttgaactc accgccgacc ttgacctcgt gctggcggcc gaactgcttg gtcagatcca
     8701 gcttggtgcc gataaagctt tccgaagcct tggtgatggt gccgctggtg ccgacagcag
     8761 ggtcataagc ggtcagatcc atgaaggagt aggtcttgac cgtgccggtg tcggaatagg
     8821 ccttgttgta gagcggatcg ccccacttgt tgatatcctg ccagtaaacc ggatggccgc
     8881 tctcggcgtt gtccgcaaaa tagttaacat tccaggtgta gaacatcgtc ggattgacgt
     8941 tgtgggtcat ctggagatag gtgctgtagt tggtctgccg ccagcgcttg gaggcttccg
     9001 tgttgtagat ggtgttggtg gcgttgaggg gaacagtcgc aggtcggtcc tgggaacgct
     9061 gccaggtgta ggagccgccg accttgaagc ggagggcgcc cagatcccag accacgttgc
     9121 cgttggcgac gtcgaactgg ttgccgccgc cggggatacg gccgggggcg atgttggccg
     9181 ccaggttgag ggtgtcatat accgtggtgc cctcggtggt gcgccaggaa cccggaacca
     9241 gatactcggg gctcgtccag ccggagaagc gcgtggcatt gctgagacgg tagctgtgct
     9301 gaccggcgac gaagaaacgc aggttcttct ggccggggat caccggaccg ctcagggtgg
     9361 cgaaatagtc atcgtagccg taagagtaga gcttgttgag gccgaggatc tgatagcctt
     9421 tctctttacc atcaaccgcc cagaaatcat cggagaccgc ttcgagagag accttgtagc
     9481 tcggaccacc ggttttgatg gtggtatgga ccacgcccga attggcaaag ccgtattcgg
     9541 ccgtgaaacc acccgcctga aagtcgatga gttcgatgga gcggtcgaca atcgagatgt
     9601 tctcgccgcc gtcgacgatg gagctggtca tcatgccttc cacgtagcgc gcagtctcgt
     9661 gcgaacggcc gccgcggacg tagaggttgc cgccctgctg aacggcgccg gcgagggtgg
     9721 cgaccacgtt ctgcacagtg cgaaccggca gattcgcaag atcttcagcc cacaccgtct
     9781 tggtatagtt ggtgacgttc ttgtcgacca ggggcttttc agctacgatc gtgatcgtct
     9841 ccccttcgag ggcgacctga ggaagcttga agttggattc cgtcgtgacg tccggaataa
     9901 ccttgacgtt cgacatggag acctgggtat agccgacaaa ggtcgccttc aggacgtagg
     9961 tgccgggcgg cacgttcagt atcatgaaat tgccattaac gtccgaagtg gcgcccatct
    10021 gggtgccgac gacaagaacg ttagcgcctg gcaaagcctc acccgtttcc ttgtccagca
    10081 ccgtgccttt gatttttccg gtgttaccgg caaaaaggac aaaaggcaac aggacaagca
    10141 tgagcacaca aatacctttt tttagcattg ctttcctcct tgaacagtta cagaactgtg
    10201 tggttatggc agccctagtc cggctgtttc ctccgcttca ggttaaaagg cgatgatcct
    10261 gatgcttcag aggagtctgt agccaaaatc ctgaaacttt ctgcacctcc tttgcgttct
    10321 atgatggaga gaatgagtgg attgagatgt gaatatgtga ttggtcttcg tgttgcgttg
    10381 tcgctctgga tcgtcagttt gcagccataa aaaaaatgag gctttattgc agcagccaga
    10441 aaatgatgct gccccgcaaa agaccccccc agcaaaaaac tatgctcccg gcgctgtatt
    10501 attgccggat gatccatcaa caagaggcac tgccgccatt gaaagggacg gcaagggaat
    10561 agttgcagtc aaatatctat gtatatagag gttatacagg atgtttgagt tcgtcgatta
    10621 ggtcttttaa tatcaattat ttacaattgc tctgttcacg ctgaagatta tgcgccctac
    10681 gttttcgcaa atatacacaa attcttgatt tccgtcaagt tttttttaca aacccgaact
    10741 acatcccctc tcccctagcg ctccagcgcc ccctgcagca aacgccagac ctcctccttg
    10801 ccccggccgc ttttggctga aaaaggcact atctttccaa aatccccctc ccccagggct
    10861 gcgcgcagcc ccgccagatt cttctgcagt tcgctccccg agagcttgtc cgccttggtc
    10921 gccaccacaa tgcaggaccg cccctgcagc cgcagccact ccagcaactg ggcgtcgagc
    10981 tcctggaggg agaggcgcac atccaccagc accaccacca gccggagttc ttccaccccg
    11041 gtcagatagg actcgatcag tccctgccac tgctgacgga ggcttttcgg ggatttggca
    11101 tagccatagc caggcagatc cacaaaatag aactgacgat tgacgcgata aaaattgagg
    11161 gtctgggtct tgccgggggt tgaactcaca taggcgagat tgcgccggtt gaaaaggacg
    11221 ttgagcaggg tcgatttgcc gacattggag cgtccggcga aggcgatctg gggcagccgg
    11281 tcccggggca gatcctccag ccgagccacc gattttatat attcggccga gaggatcttc
    11341 acgccgccga ttcctgcacc ggattttccg gcggaagctt gcgtttcttg taacgcggtt
    11401 cggcgccgct ggtcacgacc gcctcggtaa tgcggcagtg ggagatctcc ttccgcgagg
    11461 gcagctcgta catgatcctg agcatggtct cctccagcac cgagcgcagg ccgcgcgccc
    11521 cggtgccgcg cttgcgcgcg atacgcacga tctcctgcag ggcggcgcgg tcgaattcga
    11581 gttcgacccc gtcgagggcg agcagacgct ggtattggcg ggtaagggcg ttgcggggtt
    11641 cggtcagaat cctcagcagg gcctcgtcgt cgagctcctc gagggtgcag atgatcggca
    11701 gtctgccgat cagttcaggg atgaggccgt actggagcag atcttcgggc tcgacccggc
    11761 gcagcagctc gcccagctcg agggcgtcgc ccttgacgct ctcggccccg aagccgatgg
    11821 ccatcttgtt gaggcggctg gcgatgagct tctccaggga ttcaaaggcc ccgccgcaga
    11881 tgaagaggat gttggcggtg ttgatgttga tgaattccat ctcgggatgc ttgcgccccc
    11941 ccttgggggg aacggcagcg atggtgccct caagaatctt gagcagggcc tgctggaccc
    12001 cctcgccgga gacgtcgcga gtgatcgagg ggttgccgtt cttgcgcgcg atcttgtcga
    12061 gttcatcgat atagacgatg ccgcgctcgg cgcgttcgag gttgtaatcg gccgcctgca
    12121 gcagccgcac caggatgttc tccacatcct cgccgacata gcccgcctcg gtcagggtgg
    12181 tggcgtcggc gatggtgaag ggcacctgca gaaggcgggc gagggtctgg gcgatgaggg
    12241 tcttgccggt gccggtcggg ccgatgagca ggacattgct cttttcgagc atgacctcat
    12301 cggccggggc ggcatggttg acgcgtttgt agtggttgta gaccgccacc gcgaccgtct
    12361 gtttggcgtg ctcctggccg atgacgaact tgtcgagttc ggccttgatc tcagcggggg
    12421 tggggacgcg ctcgaccgtg gccgtggtgc gccgcgattt ttcccgggcg atgatcttgt
    12481 tggcgatggt cacgcattcg ctgcagatgg tcacgtccgg gccggtgacg atggtctcca
    12541 cctgctggct gcttttgccg cagaaggagc agatcaccag cctccggccg ccgttctgtt
    12601 ttttttgatc cataatactt tgccgtccgc taagagtcta tttctttttg ggtttgacga
    12661 ggtccttgcg caccatgatc tcgtcgatga ggccgtactc tttggcctcc tccgcgctca
    12721 tgaaaaagtt gcgatcggtg tccttgacga tccgctccat gggctggccg gtatggtgga
    12781 cgagtatctg gttgagtttc tcgcggatga ccaggatctc cctggtctgg atctcgatgt
    12841 cggtggcctg tccctgggcg ccgcccatgg gctgatggat catcacgcgc gagtggggca
    12901 gggcgaagcg cttgcccttg gcgccggcgg ccagcagcag ggcgcccatg ctcgaggcct
    12961 ggccgatgca gatggtggag acgtccggtt tgatgtactg catggtatca taaatggcca
    13021 gccccgagct gacgatgccg cccgggctgt tgatgtagag atagacatcc ttggccggat
    13081 cctcggcggc caggaagagc agctgcgcaa tggccaggct ggccacattg tcatcgatgg
    13141 ccgaaccgag gaagactatg cgctccttga gcaggcgcga aaaaatatcg taggctcgct
    13201 cgccgcgtcc ggtctgttca acgactatcg gtactagtcc catagggtct ccttcatgct
    13261 tgggcggatg gatgttcgtg atcgtggtgc tggtagagcg agcgccggac ctctgtcact
    13321 ttggcttttt ggctgaggaa ggcgtagacc ttgtcatcct cgagggcgtc gcgaaagcgt
    13381 tctcgctcct cctttttgct gcggatctgc tcggcgcgct gggcgccctc ctcgcctgca
    13441 gcggccatgg cggagagggc cgattccagc tcctcatcgc tgacggccag cccctgctgg
    13501 ctgatgaggc ggtcgcggat gagataccac ttgacgccgt gcaccgcgga ggggcggtag
    13561 tgttcagcca ggtgggcctc gtcgacggat tccttgctct tgcgcttgac gtcggcgatg
    13621 agcatggcca gatagttatc gaccatcgag ggcggagctt cgatgggggt ctgcttgatc
    13681 agctcatcgg caagggcgtc gcggaagcgc tcgtgggtct ggtgctccgc ctgggcctgc
    13741 agccgggcgc ggatgtcggc cttgagctcc tcgaggctgc tgaaggcccc gagatccttg
    13801 gcgaattc
//
LOCUS       DAOPWU010000055        13673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4589_length_13673_cov_5.003451, whole genome shotgun sequence.
ACCESSION   DAOPWU010000055 DAOPWU010000000
VERSION     DAOPWU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13673
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4589_length_13673_cov_5.003451"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PKM23_04735"
     CDS             <1..725
                     /locus_tag="PKM23_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90811.1"
                     /translation="DIFAWPADAAGGKEWTLRMTTLIREIDPNHPVTCGLHVASLLED
                     NGLRVNDVFNEVDIPVMHGYPMYADWSNGPLDSDFVPFLCALTTALCGKPSLMEEFGG
                     CTAAPGQASFVHEWQRYGNPFRQFMASEEDLAAYIEAVLPKLVEVGALGAMIWCYADY
                     IPALYDRPPCDQAWHERFFGLVRPDGSLKPHAEVIKRFAASRPVVQPARRPVSLDVTP
                     EAYYQAPKRHAVRLYHTFSGGR"
     gene            complement(735..1676)
                     /locus_tag="PKM23_04740"
     CDS             complement(735..1676)
                     /locus_tag="PKM23_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HNY90812.1"
                     /translation="MSHADFKELFTRHPQNPVIVTADLPYQANTVFNAAAALVGGETL
                     LLIRIEDRRGISHLTVARSKDGVGGWRIDPQPTFPPDPDHYPEELWGVEDPRITWMED
                     LGEFYFTYTAYSQDGPLVALARTKDFRSFTRLGGVLLPENKDAALFPRRINGRYAMLH
                     RHVLHSGAHIWIAFSPDLKHWGDHQIVMRARSGGWWDANKIGLSPQPIHTPEGWLVLY
                     HGVRVTGGGVIYRLGLALLDAEDPRRLLRRSEEWIFGPMENYERQGDVDNVVFPCGWT
                     QQGDQLRLYYGGADTCIALATASLKELLEYLRHCPEK"
     gene            complement(1723..3090)
                     /locus_tag="PKM23_04745"
     CDS             complement(1723..3090)
                     /locus_tag="PKM23_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90813.1"
                     /translation="MLTIRRIVLFPLLVLACTRAPEEAILARTGGRIITRAEFVERAE
                     FSPEVQFQGPESARAGYLLSRLIDEKLAAQSAEKAGLDTTATLQGLTGFIEEMAMARE
                     LFRVEVREKVTLDPAEIGLAVQQQAQVRKVGYLVFEDESLARHHQQRLAAGESFNGAL
                     RALYGAAADTTANRRLIRWGENEPVIEEAAWALAPGQTSGIIEVDGALMVMRLEEVTA
                     SPALTETDLEQRTRRARTIMRSRREAVESDRFIAAFALEKRLQFNRQLVGQAAKVLAG
                     LEESRPGASGTLPAERPAAVEIVHGAQEALAAELATPLATWNGGGITLGELLERWQGV
                     NFSSGQSTPPARRRTIVHSFSLIVRDAMLAQEARRRGLDKSAAVRSEVRIWRDYYLAL
                     AWQEEMSRQGSSAAAALGQLRRENPVQVDSVMMRGIELSPVPLLALRPGQYNARVAPP
                     WIVFD"
     gene            complement(3111..6638)
                     /locus_tag="PKM23_04750"
     CDS             complement(3111..6638)
                     /locus_tag="PKM23_04750"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNY90814.1"
                     /translation="MLIPAKREFFLLTMLFGLPLLAQEELILQQGRNGYAGCQDAHIH
                     ANKPDWNTGGEEGLEATGNGGEADAKHLLLRFDLSTLPAESRIDSAWIELYLAKRRTP
                     QTATKTLAVYRLNRPWNEGSGDDTGGYDGRPAAGGEVCWSHAVYPDAPWSLPGAYGVP
                     HDHNPLAEDERPIDPADPAGHWYSWRVTALTQAWLSQPDSNFGLLLREPSVSAAAGIL
                     NFASSQFSADSLRPRLRLLLANSSPQTIAREMTATASAGAITVQWPCRGDANGDGYAE
                     IALARAGDAQWGARQRMDKTSNGYDYTFTGLQFGRYHVRGWISDPDGVQGDGIQTLLN
                     LELSPDASRSGSLYTMLQSDNSVQVELTFSDDGDADNSALLAHKLSSSPLWQEDGPMT
                     RAPGRFSKLLTGLSGGETWDLRVTLRDPDGITGDSIFTAQLYIPPATGPVRLLSQGHK
                     HFELQSGAFTALYDSVHTGAALWLSSALGRQYVVRNTIANGLPFAVLDPALIDSIGIK
                     SDGECECIEVTLHGHWGSLRHRVTVTVFINHPGLFQWQCSLSGAAGINIPNGAAECTF
                     FDRERNYTVSSNLLSWVQQAPFAAGLASGYDPLALQGTLLYFQDFTSLNDYFTLQRTA
                     PRECVRCTSTGFGFERPGGNRPLRSEPTILTSGWLFLAPGKPASESAMAEGFISALAD
                     IYALIPKPAIGETDWKEITGGLLADLDDERCLSTVHGQRFLRSYVGVPRLDNAEAITQ
                     LDVLVALRRFEEVHGEVSALDDHLADNLWQFYNTRLNTMVNDAPNEGVYEGDSWYALH
                     IHLGLARLARMGDGSARTLLFLSLPKLMRFARSVNYDFPVFFRYTDDSAISGHEYDVA
                     GAYVYLMLDAWQLSGSEEYLQEAKLAAEHIAGHGFEYIYEAHMTAATCAALARLFRIT
                     GESRYLELSYMPFANLIAISWLWECDYGYAAEYSTFFGLSPMAGAAVITPMEQHQSWS
                     YLREYRQLAASGLPAGLLQLLDGFIAYTPAVMKYTLPPFLPAAALVTGPTIYDSWNVP
                     GFHIPLEDLREGWQKSGSLGQQIYGAGASLIFAAELTTGIRAEEEELSQPRPALLENY
                     PNPFNAGTRISWQLPEAGRNAKVSCSLTIYDILGRKVRVLPVNTISGQGEAFWDGRDD
                     GAAAAGGGVYLIVLEGEGRRLVRKVLLLR"
     gene            complement(6658..7992)
                     /locus_tag="PKM23_04755"
     CDS             complement(6658..7992)
                     /locus_tag="PKM23_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90815.1"
                     /translation="MALVLAALGSSCSSSPRSPVNFAHLDRLCQEVTVAGQPCTIVHI
                     YSDAPDYGWTDAAGEGIACVDDVARAAVVYMRAGDARHLERIRSLLRFVLTLQNKDGT
                     FCNFVHQDLTINREGPTSYASFGFWAARGYWALGEGCAFFRERDPAFAAELRSAFHRC
                     LPQLEALALAYGSFESIGGKAWPNWLLNRHAADATSEFLLGAAAFLAVEPDTALQRHA
                     ARLAEGLCAMQAGREPLAGAFYSWPGVWHGWGNAQIQALPPLSSQLDGPELLRRAEYG
                     GRAFLGRMLAGGWLHEYDFSTGRAAVFSQIAYDVRTTALSLLALHHATGNPEYARLAG
                     IASSWLTGNNIAGEPLYDAATGRGYDGIDRKGVNRNAGAESTIEALYTLIEIEKVPAA
                     AAWLQAKSSEKWNETAWVPIQELRRTFRTAQGALRLIWHAESGDYTLQRLNP"
     gene            complement(8019..8828)
                     /locus_tag="PKM23_04760"
     CDS             complement(8019..8828)
                     /locus_tag="PKM23_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY90816.1"
                     /translation="MKRALIWLILILGTVVFLYPFLWMVSATFKPEMEIPSFGLLPSS
                     ITLASYEQVFAKIPILRSLLNSLVVSISITASVLFFGSMVGYALSRLRFRGREVMVMV
                     ILFTMMIPFQLTMIPLYILMVKLHWTDTYLALIVPGMMSGFGILLFRQFFKSVPQALI
                     DAARMDGLSDLQILFRIIWPLSRPVLITVGIITFMNSWNDVLWPLIVIRDQSIMTMPQ
                     MVTIFAVSGMAEAQLGVKLAAATLLALPVIIAYAFFQRYFIESLASTGLKG"
     gene            complement(8825..9712)
                     /locus_tag="PKM23_04765"
     CDS             complement(8825..9712)
                     /locus_tag="PKM23_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY90817.1"
                     /translation="MKVERKIAGNRSSRTGFLLSLPYWAWFAFFMAWPLLFSLVLVFH
                     EWDIYTPMRWVGLGNFVRLFQDDLFWQAILNTLYFLVIHIPLQIGLALLFAVLLNEKI
                     RARGFFRAAYFMPVVVSGIVISLLWQQLFSYENGILNLLLARAGLARIPWLTSPEWAM
                     PSVALMATWKNVGLYIILFLAGLQNIPAWLYEAAGIDGATAWQKFRHITLPMLNPAVI
                     MVLILSTIGGFSLFIEPYVLTGGGPMNRTLSAMLYIYKQAFYFNRMGYAATLGFFFAF
                     IILAVVLVQRKVVEQEALE"
     gene            complement(9753..11021)
                     /locus_tag="PKM23_04770"
     CDS             complement(9753..11021)
                     /locus_tag="PKM23_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY90818.1"
                     /translation="MLVLSAGVLLVGCESGSRGKAGHAGLVYWPAANAQEIELAGLAA
                     AEWNAAHPDVPVTVQPIPESQSTEEVLLAAVVGKTTPDVCSNIWPGVVGQFVRAGALV
                     ALDSFADFDSVAAARLPEGQREVFQYDDGRLYQMPWKSNPIMMEYNVRRLQEAGFTHP
                     PATYSEFIRAGQALTRDLTGDGQPDQWMLYVDINVKWWLRYFDFYTFYLAASGGQTLI
                     KNRQVTFDNRAAVEVFRFFRRCFELGIFPRASFQGDTFLAGQVATHITGPWNIAHVDK
                     FKPAGFEFDYAPVPLPDSAAGPVVTYGDPKNIAIFATCQNPRAAWEFVKFLVSRRQDL
                     ALLRIASQLPIRRDLLSDSLYMPYFKEYPKMVRFAEQAPRTRGVDSAPELREVFDAIS
                     QEFEACCVLGRRTPEEAVQRAAERARQVLR"
     gene            complement(11080..13590)
                     /locus_tag="PKM23_04775"
     CDS             complement(11080..13590)
                     /locus_tag="PKM23_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90819.1"
                     /translation="MIGKLSHRTAVVGLVLLGSFAACGAGEKEESWRSLSVNFNEMRG
                     QIEVGGPYAGAEFHDSRPLPARISFYYPVANSIDLSTDYWRRGESRPLRFALKHDGRA
                     DSLGAAPWAYRYTPWWIEFQQHNPEYLCSIRYRFCRTLPVMVLQLELTNAKDRARSFE
                     LEGTLEWILRTCQTYAWMRPARVACALQNPAVPASGCTLVTAAWDAPETGSALVFVAN
                     AGALPAASAGVDSLHLSYQKKLRPGEEWRIVLLIGSCRHGEAEAVAARAFREWQDEAT
                     AFEEEAGRYAAGPLLQVPDAGLTETAMWSRAVMAANRHYLDGRIVPMPCPAEYNFFFT
                     HDLLLTDLGAVFFDCERVRNDLLYVQSLARADSVLPHAYYWRDTGFQTEFCSTDNWNH
                     LWFILLSASYLRHSGDTATMQLLAPMLTKSLRMMLENRGADDLMYAWQPDWWDIGHSW
                     GARAYITILTVRALREYAFMGGQLGWPEAELTRHLELAERLQQQLGERLWDDEAGFLF
                     NGLAGGATDRHYYAGSLLAAAWKLLDEERSARLMKTAAAELLDRCLGIRIAAPMDFDQ
                     YDELYEFLPGQVGAPGLYLNGGVWPHGIVWYGLGWLAAGQPDSAREVLKQYLTLEGIR
                     HSPLGQPAFFEYRNADPASPRYGEIDKPTFLWTGGWFLHALYQLAGMRESEWNLSFDP
                     GLPQEWRKLRYEVMLNGRRVEVAWRGEGRWFRQIKADGRPLHSAVIPASADASPLRRL
                     VLERGEPHTPYLASANTAVESVELDSRNKSLRIKVPGFAGRTVELAIVSPWEPLQEAS
                     AGREISVTRHGRVREILLRARLKEGREEIDINFNKGRD"
BASE COUNT         2581 a         4180 c         4558 g         2354 t
ORIGIN      
        1 cggacatctt cgcctggccc gccgatgccg ccggggggaa ggagtggacg ctgcggatga
       61 caacccttat ccgcgagatc gatcccaacc atccggtcac ctgcggactg cacgtcgcca
      121 gcctcctcga ggacaatgga ctgcgggtga acgatgtctt caacgaggtc gatatcccgg
      181 ttatgcacgg ctatccgatg tatgcggact ggtccaatgg cccgctcgac agcgatttcg
      241 tcccttttct ctgcgccctg accacggcgc tctgcggcaa gccctccctg atggaggagt
      301 tcggcggctg caccgcggcg cccggccagg cctccttcgt ccatgaatgg caacgctacg
      361 gaaatccttt tcgccagttc atggcctccg aagaggatct tgcggcctat attgaagcgg
      421 tgctgcccaa actggttgag gtgggcgccc tgggtgcgat gatctggtgt tatgccgact
      481 atatccccgc cctgtatgac cgtccgccct gcgatcaagc ctggcatgag cgttttttcg
      541 gactggtgcg gccggacggc tcgctcaagc cgcacgcgga ggtgatcaaa cgcttcgccg
      601 cttcccgtcc ggtcgtccag cccgcgcggc gcccggtttc acttgatgtg acgccggagg
      661 cctactatca ggcgccgaag cgccacgcgg tgcggttgta ccacactttc agcggcggcc
      721 ggtaacccgg gcggtcattt ttccgggcaa tggcggaggt attcgagcag ttccttgagg
      781 ctggcggtgg cgagggcgat gcaggtgtcg gccccgccat aatagaggcg cagctggtcg
      841 ccctgctggg tccagccgca ggggaaaacg acattatcga catcgccctg gcgttcatag
      901 ttctccatcg ggccgaaaat ccactcctcg ctgcggcgca gcagtcgccg cggatcctcc
      961 gcatcgagca aagccagtcc cagccgatag atgaccccgc cgccggtcac gcgcacgccg
     1021 tgatagagga ccagccagcc ctcaggggta tggatcggct gcggcgagag gccgatcttg
     1081 ttggcgtccc accagcctcc ggagcgggcg cgcatgacga tctgatggtc gccccaatgc
     1141 ttgaggtccg gtgaaaaggc gatccagatg tgggcgccgc tgtggaggac atggcggtgg
     1201 agcatggcat agcggccgtt gatgcgccgg ggaaagaggg cggcatcctt gttctcgggc
     1261 agcagcacgc cgccgaggcg cgtgaagctc ctgaaatcct tcgtacgggc cagcgcgacc
     1321 agggggccat cctgggagta ggcggtgtag gtgaagtaaa attcccccag gtcctccatc
     1381 caggtgatgc gtggatcctc cacgccccaa agctcctccg gataatggtc gggatcgggt
     1441 ggaaaggtgg gttggggatc gatccgccag ccgccgacgc catccttgct gcgcgccacc
     1501 gtcagatgcg agatgccacg ccggtcctcg atgcggatca gcagcagggt ttcgccgcct
     1561 accagagccg ctgcggcgtt gaagacggtg ttggcctgat agggcagatc cgcggtgacg
     1621 atgaccggat tttgcggatg gcgggtgaag agctccttga aatcagcatg tgacatagtg
     1681 tggcctcttg aaaagttcgt ttgacctgaa cgccagtgat actcaatcaa aaacaatcca
     1741 tggcggcgcc acccgggcgt tgtattgccc gggccgcagc gccagcagtg gaacggggct
     1801 gagttcgatc ccgcgcatca taacactgtc gacctgaact gggttctccc ttctcagctg
     1861 tcccaacgca gccgctgccg agctgccttg ccggctcatc tcctcctgcc aggccagggc
     1921 gagatagtaa tcccgccaga tacgcacctc tgagcgcacc gctgccgatt tgtccagccc
     1981 gcgccggcgc gcctcctgcg cgagcatggc gtcgcgcacg atcagactga agctgtgcac
     2041 aatggtgcgg cggcgggctg gtggggtgga ctggccggat gagaaattca cgccttgcca
     2101 ccgctccagc agctctccga gggtgatgcc cccgccgttc caggtggcca gcggcgtcgc
     2161 cagttcggcg gcgagcgcct cctgcgcccc gtggacgatc tcaaccgcag ccgggcgctc
     2221 cgccggcagg gtgccggaag cgcccggccg cgattcttcc aggccggcca gcacttttgc
     2281 ggcctggccc accagctggc ggttgaactg cagtcttttt tcgagggcga aggcggcgat
     2341 gaaacggtcg ctttccaccg cctccctgcg gctgcgcata atggtgcggg cgcggcgggt
     2401 gcgctgttcc agatcggttt cggtcagcgc cggcgacgcg gtgacctcct ccaggcgcat
     2461 caccatcaag gcgccgtcga cctcgataat ccccgaggtc tggcccggtg cgagggccca
     2521 ggctgcctcc tcgataaccg gctcgttttc cccccatcgg atcagccgcc ggttggccgt
     2581 ggtatccgcg gcggcgccgt agagggcgcg cagcgcacca ttgaaacttt ctccggccgc
     2641 cagccgctgc tgatggtggc gcgccaggga ttcatcctcg aaaaccagat agcccacctt
     2701 gcggacctgc gcctgctgct gcacggcgag gccgatttcg gccggatcca gggtcacctt
     2761 ctcccgcacc tccacccgga agagctcccg cgccatggcc atctcctcga tgaatccggt
     2821 cagaccctgc agggtggctg tggtatccag gccggccttc tctgcggact gggccgccag
     2881 cttttcgtcg atcaaacggc tgagcagata gcccgcccgg gccgattcgg ggccctggaa
     2941 ctgcacctcc gggctgaact cggcgcgctc gacgaactcg gcgcgggtga tgatccggcc
     3001 gccggtacgg gcgaggatag cctcctccgg cgccctcgta caggccagaa cgagaagggg
     3061 gaatagaaca attctgcgga tcgtcagcat attcatcctt gttcgggcgc tcaacgcagc
     3121 agcagcacct tgcgcaccag ccgccggcct tcaccctcga gaacgatcag atagacgccg
     3181 ccgccggctg ccgctgcgcc atcatcgcgg ccatcccaga aggcctcgcc ctggcccgaa
     3241 attgtattta ccggcaatac ccggactttg cggccgagaa tatcatatat ggtcaggctg
     3301 cagctgacct tggcattgcg gcccgcctcc ggcagctgcc agcttatgcg ggtgccggca
     3361 ttgaagggat tgggataatt ttccagcagc gccggccggg gctgagacag ctcctcctct
     3421 tcagctctga tgccggtggt cagctcggct gcaaagatca gggacgcgcc ggcgccgtag
     3481 atctgctgcc cgagcgagcc cgatttctgc cagccctcac gcaaatcctc cagagggata
     3541 tggaatcccg ggacgttcca ggaatcgtaa atcgtaggac cggtgaccag ggcagcagcg
     3601 ggcagaaacg gcggcagggt gtatttcatc accgccgggg tgtaggcgat aaagccgtca
     3661 agcagctgca gcaaacccgc gggcaggccg gaggctgcga gctgacggta ttcgcgcaga
     3721 tagctccagc tctggtgttg ttccatgggc gtgatcacgg cggcccctgc catcgggctc
     3781 aaaccgaaaa aggtggagta ctccgcggca tatccgtaat cgcactccca gagccaggag
     3841 attgcgatca gattggcgaa gggcatgtag ctgagttcca gatagcggct ctcgccggtg
     3901 atccggaaga gcctggccag tgcggcgcag gtggcggcgg tcatgtgggc ttcgtaaata
     3961 tactcgaatc cgtgaccggc aatatgctcc gccgccagct tggcctcctg gagatactcc
     4021 tcgctgccgc tgagctgcca ggcatcgagc atgaggtaga cataggcgcc ggcgacatcg
     4081 tattcatggc cggagatggc cgaatcgtcg gtgtagcgga agaagaccgg aaaatcataa
     4141 ttcaccgacc gggcgaagcg cattaatttg gggagggaga ggaagagcag ggtgcgtgcg
     4201 ctgccgtcac ccatgcgcgc cagccgcgcc agtccgagat ggatgtgcag ggcataccag
     4261 ctgtcccctt cgtagacccc ctcattgggc gcatcattga ccatggtgtt gagccgtgtg
     4321 ttgtagaact gccagagatt atccgcgagg tggtcgtcga gggcgctcac ttcgccgtgc
     4381 acctcttcga agcggcggag ggcgacgagc acatcgagct gggtgatggc ctcggcgttg
     4441 tcgaggcgtg gcacgcccac ataggaacgg agaaagcgct ggccatggac cgtcgagagg
     4501 cagcgttcgt cgtcgagatc agccagcagc ccgccggtga tctctttcca gtcggtctcc
     4561 ccgatggccg gtttggggat cagcgcatag atatcggcca gcgcggagat gaatccttca
     4621 gccatggcgc tctccgaggc tggtttgcct ggggccagga agagccagcc actggtgagg
     4681 atggtcggct cgctgcgcag gggccggttc ccgcccggac gctcgaagcc gaatcctgtc
     4741 gaggtgcatc gtacgcactc ccgcggggct gtccgctgca gggtgaagta atcgttgagc
     4801 gaggtaaagt cctgaaaata gagcagcgtg ccctgcaggg cgagaggatc gtagccgctg
     4861 gccaggccgg cggcgaaggg agcctgctga acccaggaga ggaggttgga gctgaccgtg
     4921 taattgcgct cccggtcgaa aaaagtgcat tcggccgcgc cattggggat gttgattccc
     4981 gcggcgccgg agagtgaaca ttgccactgg aagaggccgg gatggttgat aaagaccgtc
     5041 actgtcaccc ggtggcggag gctgccccag tggccatgca gtgtgacctc gatgcattcg
     5101 cattctccat ccgacttgat cccgatgctg tcgatgagcg ccgggtcgag cacggcgaag
     5161 ggcaggccgt tggcgatggt attgcggacc acatactgcc ggcccagggc ggaggagagc
     5221 cacagtgcgg cgccggtgtg gacgctgtca tagagggcgg tgaaggcgcc ggactggagt
     5281 tcaaaatgtt tatggccctg gctgagcagg cgcaccggtc cggtcgccgg cgggatatac
     5341 agctgcgcgg tgaagatcga gtcgccggtg atgccgtcag ggtcgcgaag ggtgacgcgc
     5401 agatcccagg tttcgcctcc gctcagtccg gtgagaagct tgctgaaccg tcccggtgcg
     5461 cgggtcatgg ggccatcctc ctgccagagc ggggaggagg agagcttgtg cgccagcagc
     5521 gccgaattgt ccgcatcacc gtcgtcgctg aaggtcagct ccacctgcac gctgttgtcg
     5581 ctctgcagca tggtatagag cgagccggag cggctggcgt cggggctcaa ctcgaggttg
     5641 agcaaagtct ggatcccatc gccctgaacg ccgtcgggat cgctgatcca gccgcgcaca
     5701 tggtagcgcc cgaactgcag ccccgtaaag gtgtaatcgt agccgttgct ggtcttgtcc
     5761 atgcgctgcc gggcgcccca ctgggcgtcg ccggcgcggg cgagggcgat ctcggcgtag
     5821 ccgtcgccgt ttgcatcgcc gcggcagggc cattggacgg ttatggcccc ggcagaggcc
     5881 gtcgccgtca tttcgcgggc gatggtctgc ggggaggagt tcgccaggag cagccgcagc
     5941 cgggggcgca gggaatcggc agagaattgg cttgaggcga aattgaggat accggccgcg
     6001 gccgagacgg acggctcgcg cagcagcagt ccaaagttgc tgtccggttg actcagccag
     6061 gcctgggtca gcgcggtcac ccgccagctg taccagtgcc cggccgggtc ggcgggatca
     6121 atcgggcgct catcctcggc gagggggttg tggtcgtgcg gcactccgta ggcgcccggg
     6181 agggaccagg gcgcatcggg gtagacggca tgggaccagc agacctcgcc gccggcagca
     6241 gggcggccat cgtaaccgcc ggtatcatca ccgctgcctt cattccaggg gcggttgagg
     6301 cgatagaccg ccagggtttt cgtcgcagtc tgcggcgtcc gccttttggc cagatagagc
     6361 tcgatccagg ctgaatcgat gcggctttcc gccgggaggg tgctcagatc aaatcggagc
     6421 aaaagatgct tggcgtctgc ctcgccgccg ttaccggtgg cctccagtcc ctcctcgccg
     6481 ccggtattcc agtcgggttt gttggcatga atgtgggcat cctggcagcc ggcatagcca
     6541 ttgcgcccct gctgcaggat cagctcctcc tgggcgagga gcggcagtcc gaagagcatc
     6601 gtcagcagga aaaattctcg cttcgctggg atcagcataa ggggcatccc gttcttgtca
     6661 ggggttcaac cgctgcaggg tgtaatcgcc gctttccgcg tgccagatga ggcggagggc
     6721 gccctgtgcg gtccgaaagg tgcggcgcag ctcctgtatg ggcacccacg ccgtctcgtt
     6781 ccatttttct gatgacttgg cctgcagcca ggccgcggcg gccggaactt tttcgatttc
     6841 gatgagggtg tagagcgcct cgatggtcga ctcggccccg gcgttgcgat tgaccccctt
     6901 gcggtcgatg ccgtcatacc cgcgcccggt ggcggcgtca tacaggggct cgccggctat
     6961 gttatttccc gtcagccagg aggaggcgat cccggccaag cgggcgtatt caggattgcc
     7021 cgtcgcgtga tggagcgcca ggaggctgag ggcggtcgtg cgcacatcgt aggcgatctg
     7081 ggaaaaaacg gccgcccgcc cggtgctaaa gtcgtattca tgcagccagc cgccggccag
     7141 catccggccg agaaaggcgc ggccgccgta ctccgcccgt cgcagcagct ccggtccatc
     7201 cagctgggag gagagggggg gcagggcctg gatctgggcg tttccccagc cgtgccagac
     7261 gcccggccag gagtaaaagg cgccggccag cggctcccgg cccgcctgca tggcgcagag
     7321 cccttcggcc agccgggcgg catgccgctg cagggccgta tccggctcga ccgcgagaaa
     7381 agccgcggcg ccaaggagaa actcggaggt ggcgtcggcc gcatgacggt tgagcagcca
     7441 gttcggccag gctttgccgc cgatggattc gaagctcccg taggccaggg cgagtgcctc
     7501 gagctggggc aggcagcgat gaaaggcgct gcgcagctcc gccgcaaagg cgggatccct
     7561 ctccctgaaa aaggcgcagc cctcgcccag cgcccagtag ccgcgcgccg cccagaagcc
     7621 gaacgaggcg tagctggtcg gcccctcccg gttgatggtc agatcctggt ggacgaaatt
     7681 gcaaaaggtg ccgtccttgt tctgcagggt gagcacgaag cggaggaggc tgcggatgcg
     7741 ctcgagatgg cgcgcatcac ccgctcgcat gtagacgacc gcggcgcggg cgacatcgtc
     7801 aacgcaggcg atgccctcgc cggcggcatc cgtccagccg taatccgggg cgtcgctgta
     7861 gatgtggaca atggtgcagg gctggccggc gacggtgacc tcctggcaga ggcggtcgag
     7921 gtgggcaaaa ttgaccgggc tgcgcgggct tgaagaacaa ctgctgccca gggccgccag
     7981 cacaagcgcc aatcggaccc tatttggaat aaacctcttc atcccttcag ccccgtgctc
     8041 gccaaactct cgataaaata acgctggaaa aaggcatagg cgatgatgac cggcagagcc
     8101 aacagggtgg cggccgcaag cttgaccccc agctgcgcct ccgccatgcc gctcaccgca
     8161 aagatggtca ccatctgcgg catggtcata atcgactggt cgcggatgac gatcaacggc
     8221 cagaggacat cgttccagct gttcatgaag gtgatgatgc ccacggtgat cagcacgggg
     8281 cgggagagcg gccagatgat ccggaagagt atctgcaggt cgctgagtcc gtccatacgc
     8341 gccgcgtcaa tgagcgcctg gggcacggat ttgaaaaact gccggaagag gaggatgccg
     8401 aaaccgctca tcatcccggg cacgatcagg gccagatagg tgtcggtcca gtgcagcttg
     8461 accatgagga tgtagagggg gatcatggtc aactgaaagg ggatcatcat ggtgaaaagg
     8521 atgaccatca ccatcacctc gcggccgcgg aaacgcaggc gggagagggc atagcccacc
     8581 atggagccga aaaagagcac cgaggcggtg atggagatcg agaccaccag gctgttgagc
     8641 agagagcgca ggatggggat cttggcaaag acctgctcgt agctggccag ggtgatgctg
     8701 gagggcaaaa ggccgaagct ggggatctcc atctccggct tgaaggtcgc cgacaccatc
     8761 cagagaaagg gatagagaaa aacgactgtc cccagaatca ggatgagcca gatgagtgcg
     8821 cgtttcattc gagggcctcc tgctcgacca ccttgcgctg cacgagcacc accgccagga
     8881 tgataaaagc gaagaaaaag ccgagggtgg cggcatagcc catgcgattg aagtaaaagg
     8941 cctgcttgta gatatagagc atcgccgaca gggtgcggtt catcggtccg ccgccggtga
     9001 gcacataggg ctcgatgaag aggctgaagc cgccgatggt cgagaggatc aggaccatga
     9061 tcaccgccgg gttgagcatg ggcagggtga tatggcggaa tttttgccag gcggtggcgc
     9121 cgtcgatccc ggccgcctca tagagccagg ccgggatatt ctgcaggccg gcgaggaaga
     9181 ggatgatgta gaggccgaca tttttccagg tcgccatcag ggccaccgag ggcatcgccc
     9241 actcgggact ggtcagccag gggatcctgg ccaggccggc gcgggcgagg aggaggttta
     9301 aaatgccatt ctcgtaggag aagagctgct gccagagcag ggagatgaca atccccgaga
     9361 ccacgacagg catgaaatag gcggcgcgga agaacccgcg cgcgcggatc ttttcattca
     9421 ggagcacggc gaagaggagc gccagaccga tttgcagggg gatatggatg accagaaaat
     9481 agagggtgtt gaggatcgcc tgccagaaaa ggtcatcctg aaagaggcgg acaaaattgc
     9541 ccaggcccac ccagcgcatc ggggtgtaga tatcccattc gtgaaaaacc agcacgagcg
     9601 aaaagagcag cggccaggcc atgaaaaagg cgaaccaggc ccagtagggc agagaaagca
     9661 gaaagccggt gcggctgctc ctgttgccag caatttttcg ttctactttc aatccattct
     9721 ttctcccggc ggaccgggtc tctgatcaac actcagcgca ggacctgacg tgcacgttcg
     9781 gcggcgcgct gcaccgcctc ctcgggggtg cgccggccga ggacgcagca ggcctcaaac
     9841 tcttgcgaga tcgcatcgaa gacctcgcgc agctcgggag cggagtccac cccccgggtg
     9901 cgcggggcct gttcggcaaa gcgcaccatt ttgggatact ctttgaaata gggcatatac
     9961 aacgaatccg ataataaatc acggcggatc ggcagctggc tggcgatccg cagcagcgcc
    10021 agatcctgcc ggcgcgagac cagaaacttg acgaattccc acgccgcgcg gggattttga
    10081 caggtggcaa agatggcgat gtttttcggg tcgccatagg tcaccaccgg acccgcggcg
    10141 ctgtcgggca gcgggaccgg cgcgtaatcg aattcaaaac ccgccggttt gaatttgtcg
    10201 acatgggcga tgttccacgg gccggtgatg tgcgtggcca cctggccggc caggaaagta
    10261 tcgccctgaa aggaggccct ggggaagatg ccgagctcga aacagcggcg gaagaagcgg
    10321 aagacctcga ctgcggccct attgtcaaag gtcacctgcc ggtttttaat cagggtctgg
    10381 ccgcccgagg ccgccagata aaaggtataa aaatcgaagt agcgcagcca ccatttgacg
    10441 ttgatatcga catagagcat ccattgatcg ggttggccgt cgccggtcag atcgcgtgtc
    10501 agggcctggc cggcgcggat gaattcggag taggtggcgg gagggtgcgt gaaaccggct
    10561 tcctgcagcc gccgcacatt atactccatc atgatcggat tgctcttcca gggcatctgg
    10621 tagagccggc cgtcgtcata ctgaaagacc tcgcgctgcc cctccggcag ccgcgcggcg
    10681 gccacggaat caaaatcggc aaaggaatcc agcgccacca gagccccggc gcggacaaac
    10741 tgtccgacca cccccggcca gatgttcgag cagacatccg gggtggtttt gcccaccact
    10801 gccgccagca gcacctcctc ggtggactgg ctctcgggga tgggctgtac ggtcaccggc
    10861 acgtccgggt gggccgcatt ccactccgcc gccgccagcc cggccagctc gatctcctgg
    10921 gcattggccg ccggccagta gaccagcccc gcatgcccgg ccttcccgcg cgacccgctc
    10981 tcgcagccga ccagcagcac tccagcactg agcacgagca taatccagaa ccggtgaagg
    11041 cagggacttt tcttaacggg gagcggcttc atgaggcgat caatccctcc ctttattaaa
    11101 attgatgtca atctcttccc gtccctcttt cagtctggcg cggagcagta tttcccgcac
    11161 ccggccgtgg cgggtcactg aaatctccct gccggcactt gcctcctgaa gcggttccca
    11221 gggcgagacg atggccagct ccaccgtccg tccggcgaag cccggaactt tgatgcgcag
    11281 gctcttgttc cgcgaatcga gctctaccga ttcgaccgct gtgttggcgg aggccagata
    11341 aggggtgtgc ggttcgccgc gctcaagcac gagtctgcgc agcggtgagg cgtcggcgga
    11401 ggcgggtatc accgccgaat gcagaggccg tccatcggcc ttgatttgtc tgaaccacct
    11461 gccctcgccg cgccaggcca cctcgaccct tcgcccgttg agcatcacct cgtagcgcag
    11521 cttccgccat tcctgcggaa gcccgggatc gaaggagagg ttccattcgc tctcccgcat
    11581 cccggccagc tgatagagag cgtgcaggaa ccagccgccg gtccagagaa aggtgggctt
    11641 gtcgatctcg ccatagcgcg gtgaggcggg atccgcgtta cgatattcga aaaaggcggg
    11701 ctggcccagg ggcgagtggc ggatgccctc gagggtcagg tactgcttca ggacttcccg
    11761 ggccgagtcg ggctgaccgg cggccagcca gccgagtcca taccagacga tgccgtgggg
    11821 ccaaaccccg ccgttgaggt agaggccggg ggcgccgacc tggccgggca ggaattcgta
    11881 aagctcgtca tactggtcaa agtccatcgg cgcggcgatg cggatgccga ggcagcggtc
    11941 gagcagctcc gcagcggcgg ttttcatgag gcgggcggag cgttcttcat ccaggagctt
    12001 ccaggcggcg gccagcagcg agccggcgta atagtggcgg tcggtggcac cgcctgcgag
    12061 cccgttgaag aggaatccgg cttcatcgtc ccaaagccgc tcgccgagct gctgctgcag
    12121 gcgctccgcc agctcgagat gccgcgtgag ctccgcttca ggccatccca gctgcccgcc
    12181 catgaaggcg tactcgcgca gggcgcggac ggtgaggatg gtgatgtagg cgcgggcgcc
    12241 ccaggagtgg ccgatgtccc accagtcggg ctgccaggcg tacatgagat cgtcggcgcc
    12301 gcggttttcg agcatcatgc gcagactttt cgtcagcatg ggcgccagca gctgcatggt
    12361 ggcggtgtcg ccgctgtgac gcaggtagga ggcgctcagg agtatgaacc agaggtggtt
    12421 ccagttgtca gtggaacaga actcggtctg aaagccggta tcgcgccagt aataggcgtg
    12481 cggcaggacg gaatcggctc tggccaggct ctgcacataa aggaggtcat tgcgtacgcg
    12541 ctcacagtcg aaaaagacgg cgcccaggtc ggtgagcagg aggtcgtggg tgaaaaagaa
    12601 attgtattcg gccgggcagg gcatcgggac aatgcggccg tcgagatagt ggcggttggc
    12661 cgccatcacc gcccgggacc acatggcggt ctccgtcagc ccggcatcgg gcacctgcag
    12721 cagaggaccg gcggcataac gccccgcctc ttcctcaaag gctgtggcct catcctgcca
    12781 ttcccggaag gcgcgcgcgg cgacggcctc cgcttcacca tgacggcagg agccgatcag
    12841 caggacgatc cgccactctt cacccggccg cagctttttc tggtagctca ggtggaggga
    12901 gtcgacaccg gcgctggctg cgggcaaggc cccggcgttg gcgacgaaaa ccagggctga
    12961 accggtctca ggggcatccc aggcggccgt caccagagtg cagccggaag ccggaacggc
    13021 cggattctgc agagcgcagg cgacgcgcgc cggccgcatc catgcatagg tctggcaggt
    13081 gcgcaagatc cactccagcg tgccctcaag ctcaaaggaa cgcgcccggt ctttcgcatt
    13141 ggtcagctcg agctgcagga ccatgaccgg cagcgtccgg cagaaacggt agcgaatgga
    13201 acagaggtat tcaggattgt gctgctgaaa ctcgatccac cagggcgtat agcgataagc
    13261 ccagggcgcg gcgccgaggg aatctgcccg gccgtcatgc ttcagggcaa agcgcagcgg
    13321 gcgcgattcg ccgcgccgcc agtaatcggt gctcagatcg atgctgttgg ccacggggta
    13381 gtaaaagctg atccgggccg gcagcggccg gctgtcgtga aactctgcgc cggcataagg
    13441 cccgccgacc tctatctggc cgcgcatttc gttgaaattc acagataggg agcgccagga
    13501 ctcctccttc tcacccgcac cgcaggcggc gaaggagccc agaagtacaa gccccacaac
    13561 cgcagttcga tgcgataatt tgccgatcat gtaataccct cttccatgaa cggggctggg
    13621 ctggccgcgg gcttctggtc ggcaaccagc cggctgaggg ccattcccgg att
//
LOCUS       DAOPWU010000056        13558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4636_length_13558_cov_4.745760, whole genome shotgun sequence.
ACCESSION   DAOPWU010000056 DAOPWU010000000
VERSION     DAOPWU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4636_length_13558_cov_4.745760"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1163)
                     /locus_tag="PKM23_04780"
     CDS             complement(186..1163)
                     /locus_tag="PKM23_04780"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNY90820.1"
                     /translation="MPIVIHPCGGNWPCRLFAAESWSQRLLGFLALAEEAEPKALLIR
                     RCSGIHTLSMDRPLGIAFLDQAGGVLRVEERLAPGRVIPRVAGATEVLEWDGGGGSAP
                     PLQPGDRLELTVDAHPHASAAAWQQLLHGPANFFLALFWLGLVISSLHHWFGGANPTG
                     LGLFCYNSLLVYLFFSRRASAALSRSGLDWLVALSTVLLSFSLRPAVDTAPRLQIPSL
                     VLQSLALIAVVYALASLGRSFGVVPANRSVKTGGAYRLVRHPLYAAELVFLAAFLLGN
                     PSPGNILKTVLITAGQIYRALAEEKLLGQDPGYLNYKSRVRFRFIPHLF"
     gene            complement(1222..2172)
                     /locus_tag="PKM23_04785"
     CDS             complement(1222..2172)
                     /locus_tag="PKM23_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNY90821.1"
                     /translation="MRLYLALAAVFITVFFLILLILRLARARLDPTTRRVRSLTEAHT
                     PVQEEITIHDALKQTELPAGLEKLLISLGRLGGSNEKAVSRSREMLIQAGLHHEYGPV
                     ILMGLRICSLFLFAVCFYLFAYRHINQPMLAPVLLMLTLLIGFRFPDYLLRHRISRRR
                     QQIGESLADALDLLVICMEAGLGLNAALLRVAQELGMRAPILSQELLVVTQEMRTGLS
                     REKALRNLSTRNRVENLRILVGALVLADKLGTSLADTLRAQADSLRTRLRQSAEEQAA
                     KAGIKMLFPLILFIFPALFIVILGPGIISILKTFAGMSGP"
     gene            complement(2181..3149)
                     /locus_tag="PKM23_04790"
     CDS             complement(2181..3149)
                     /locus_tag="PKM23_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNY90822.1"
                     /translation="MILLMSALFLFFLFFVLALLLVVYERKAGSKRKIDRRLAAYAVE
                     AGINPARMPFVLRDERLSDISLFNRLLSGLDFPARLRLLLEQAGSKYNVGMVLLTSAA
                     LGACGLLLSSGAPGVLLRVVVTLGAAALPLLYILVLRTRRLRAFIRAFPDTLDMMTAS
                     LRAGHALTKALQMVAIEAPEPVCIEFRKTFEENNLGLPLRDALVNLTQRVNSLDLKLF
                     VTAVLLQRETGGNLAEILEKISYTIRERFKLMGQIRTYTAQGRMTLWVLGSLPLVALL
                     ALQVINPTYLEPMLSERFGRTLLTIGFVLQVVGFLVIRRLIAIRYQ"
     gene            complement(3146..4450)
                     /locus_tag="PKM23_04795"
     CDS             complement(3146..4450)
                     /locus_tag="PKM23_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNY90823.1"
                     /translation="MQSQHAYHEFKERIHRRLVEKLDLAKLDTIPQETLRTLVREVIE
                     SLLSADEEVSFELNREGLTEEILNETFGLGPLEPLLHDPTISDILVNTCSQVYVERFG
                     KLETTNTRFKDDVHLLNIIERIVSKVGRRVDESSPMVDARLPDGSRVNAIIPPLAIDG
                     PILSIRKFGLQRLAMDDLLRLRSLTPAIAELLAAAVRARLNILVSGGTGSGKTTLLNI
                     LSGFIPSGERIITVEDAAELQLQQRHTVRLETRAANIEGRGAIVQRDLVRNALRMRPD
                     RIIVGEVRGAEALDMLQAMNTGHDGSLTTLHANSPRDALRRLETMVLMASANLGDRAM
                     REQISSAIHIIVQISRLSDGSRKVVKVVEIVGMEGETISLQDIFLFEKLGIREDGQVV
                     GTFRTTGIRPKFIEQIETAGIRMPDEMFEPRFELPMEEESAP"
     gene            complement(4565..5758)
                     /locus_tag="PKM23_04800"
     CDS             complement(4565..5758)
                     /locus_tag="PKM23_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNY90824.1"
                     /translation="MAKEFSWIYISENDPDTVFDALQKQEGGRLIGQCGEWLQGEALL
                     TRLQPDIALLDLGTGEEGLDYLARWSALFPRIRFFCTAGQARPELIVRAMRCGAGEFL
                     LPDADDEEISRALKSVRPGVDSGRDRPARARTVCCFGVKGGMGTTTLAVNLAVAAAKN
                     NGRRVLLIDSKLQLGNAALFLDIKPRISLLELLENLATLEVSRLDAILPGHGSGLHFI
                     AGPESMEEAEKITAAGLGRLLELLRPEYDLIIIDCDDHFDEVTIRVLDEADLILTIAQ
                     LDLSTVYNLKRTLALFRRMGYAEEKIAVVLNRYPQRISDELQSIEGSIGRSVCCRLPL
                     QENGALLESINVGEPIILSRPRHPFSLQMAELLNHIQAEGRAKNTAAPRNSRGLLRRL
                     TGRKE"
     gene            complement(5777..6757)
                     /locus_tag="PKM23_04805"
     CDS             complement(5777..6757)
                     /locus_tag="PKM23_04805"
                     /inference="COORDINATES: protein motif:HMM:NF021493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HNY90825.1"
                     /translation="MMTAKVAIRLVRQTRGAILVLAAVSMLAVMGMAALAIDAGYLFT
                     ARNQLQAGVDASALAGAAGLMTSQNLAIQQAMDFAGRNRCINQPVAVGAGDISFPNSS
                     RIRVTSSRQLPLFFASALGIPRVTISATATAELGSIIGTDHLRPFAVPDLNYRVGEYA
                     LLKAGSLDAPGTNPGFFYPVDFPPVNRGTPEVGGAAYSENIESGCDGIVEIGDIIQVE
                     PGNMVGPTKHGVEALLRWDSGAYWDNNTNSVQGSSYPGFSSPRICIVPFYDERYPPDP
                     GRNTVTVTGLGVFFIEGMQGKALYGRFIEMLTHGIWGNGNTYLYGVHLVE"
     gene            complement(6754..7830)
                     /locus_tag="PKM23_04810"
     CDS             complement(6754..7830)
                     /locus_tag="PKM23_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HNY90826.1"
                     /translation="MVRKKRILVSSRIWHWGAIALLLGFLTGAAAESDMLILEPGHSQ
                     VMEYSRGIAKVSIADPEVADANVISASQLVLIGKSEGSTSLIVWDEQGRYEQRRVLVR
                     RPMALHQVMLQVRFVEVNRIALRELGLDFLLKDHKAGDERIDVGSFAGQAASVNDPLG
                     LNEAVDLLFAVPTRNLTVMIKALQEKNLITILARPNLSAREGAEASFLAGGEFPIPIV
                     SGSVGMQTVTIQFKEFGIKLKFTPHVMDSSMINLKVAAEVSSLDFDNGITLSGFKIPA
                     LATRKAETNVDLKENHYLVIGGLLADEEARTLSRVPLLGAVPLLGRLFSSESFKKKES
                     ELMILVSPRITTGSAEMPDEGIKP"
     gene            complement(7863..8750)
                     /gene="cpaB"
                     /locus_tag="PKM23_04815"
     CDS             complement(7863..8750)
                     /gene="cpaB"
                     /locus_tag="PKM23_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HNY90827.1"
                     /translation="MFLLKLKFIIPLAMVTALLATLGAYSYLQNQKQTLEQNHAAMRP
                     VVIAKIDLPAGSKLEESLLEARLFPAALAPEGSFTQPSELAGRIVKSEIFAGEALLAS
                     RLAPPGSSGGVSAVIPPGMRAMTVAVNIVSGVSGFILPHARVDVLATVSATLDKEESK
                     TSTILENIEVLAVDQTVSPKDDDPITVKSVTLLVNPAQAEKLALASTEGKLQLVLRNT
                     ADQQTAVTEGVSLKELINKDARPTTPAAAVRRAAAAPAVREEPAKEPPKQVIEVYRSS
                     TRSEVVLEEGKATEKKPLK"
     gene            complement(8766..9152)
                     /locus_tag="PKM23_04820"
     CDS             complement(8766..9152)
                     /locus_tag="PKM23_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNY90828.1"
                     /translation="MISFVRRQEGQSLVEFALVIPIFLLLLFGIIEFGRIWETMNTMT
                     GAAREGARVAAVTAPDVGRSRAAALRLLGGVTLTGLSISVSGPGGSNEVTVTVSGTYV
                     PLTNAIIPGLIGPIPLSRTTTMHWEG"
     gene            complement(9149..9616)
                     /locus_tag="PKM23_04825"
     CDS             complement(9149..9616)
                     /locus_tag="PKM23_04825"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNY90829.1"
                     /translation="MKAHIAVVILLVVLAAVWDWRSRRIPNWLTLPALASGLLFHTFS
                     HGVQGFTASLGGAACALLIFGLVYLLGGMGAGDVKLMAAAGALLAMPAALAALFSAVA
                     AGGIIALCAMAWHRARSLRDPSGHPLPLREIRLPYGIAIAAGTLWTVVRGISP"
     gene            complement(9823..9993)
                     /locus_tag="PKM23_04830"
     CDS             complement(9823..9993)
                     /locus_tag="PKM23_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNY90830.1"
                     /translation="MQILINIFRSLFVREEGQTLAEYALILFLIAIAAVAVLGALGVS
                     ISGIFQQIIGAL"
     gene            complement(10127..10300)
                     /locus_tag="PKM23_04835"
     CDS             complement(10127..10300)
                     /locus_tag="PKM23_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90831.1"
                     /translation="MRASIHLLYEFGDDEGGQTIAEYALILLLIMVAAAAVVALFGGQ
                     LVAFFNQILAVFP"
     gene            11101..12342
                     /locus_tag="PKM23_04840"
     CDS             11101..12342
                     /locus_tag="PKM23_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNY90832.1"
                     /translation="MIKIKALRGFRPRDDIAARVAAPPYDVISSEEAREMANNNPLSF
                     LHVNKPEIDLPLSTDLYSDQVYARGAENLRRFIAEGLLRQDPAPSIYVYQQQWQGRAQ
                     TGYFCLASVDDYDAGRIRKHELTRASKEKDRTTLMDRQNAQIGPVFLMYEAEPMLDGC
                     LAETITTSPLIDFDTPEGVRHLLWRIDDTARIARIERGFAGLDKLYVADGHHRSAAAS
                     NLCRERRAANPNHTGEEPYNYFLAVIFPHTHLRILPYNRVVTDLNSLTPSEFLAAVEA
                     KFTVTPAGPAAPEVPLHAMGMYLEGLWYLIAPRPGTWDSSDAIEDLDVNILMKNLLVP
                     LLAIGDPRTDQRIDFIGGIRGAEELVRLVDSGRFKVAFAMHPTSIEQLMAVADAGKIM
                     PPKSTWFEPKLRSGLVIHLLD"
     gene            12364..13248
                     /locus_tag="PKM23_04845"
     CDS             12364..13248
                     /locus_tag="PKM23_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNY90833.1"
                     /translation="MKIVLCDKFDATLPGRLAPYGEVSDDKNLIPEADVVLIRSKTKC
                     TREFIDGAPNLKLIIRGGVGMDNVDKAYAKTKSIKAYNTPNASSIAVAELAFALMIAM
                     PNKLITAHNTTAAGQFLKSELKRTELLNKTLGIIGCGRIGLELAKRARAFGMTVFAYD
                     VVPLQSEHIHGQKSLEELLPLCDYFSLHLPLTPETTAMINKERIALMKRGAYLVNTGR
                     GKTVVEADIVEALQSGQLAGYGTDVFYAEPPEGSPLLNAPNVVLTPHLGASTAENLLR
                     IADEVVILIDKFAKGQLD"
     gene            13276..>13558
                     /locus_tag="PKM23_04850"
     CDS             13276..>13558
                     /locus_tag="PKM23_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNY90834.1"
                     /translation="MGRIYNFNPGPSTLPLAALEKAQAEFTDYKGSGMSVLEISHRSK
                     DFEAILADTQNLLKEMLGIPDNYSILFLGGGASLQFAMLAMNFIPAGGSA"
BASE COUNT         2799 a         4047 c         4210 g         2502 t
ORIGIN      
        1 tgcgccccga ggagatgacc cttctggtgg tgggggatgc gaagagcagg ctcgattccc
       61 tgagcgactt gggctacggg gttccggtcc tgctcgacaa ggagggtaaa aggattgact
      121 gagaggcaaa aaaagagctg ccctgaccgg cagctctttt tttttattac tcattcaaca
      181 gaatgtcaga agagatgggg gatgaagcgg aaacgcacgc gcgatttgta gttcaggtaa
      241 ccggggtcct gtccgagcag cttctcttcc gccagggcgc ggtagatctg ccccgccgtt
      301 ataagcaccg tcttgaggat gttccccggg ctcgggttgc cgagaaggaa ggcggcgagg
      361 aaaacgagtt cagcggcgta gagggggtgg cggacgagcc ggtaggcccc tccggtcttg
      421 acagaacgat tggcgggtac gacaccgaag ctcctgccca ggctggccag ggcgtaaacc
      481 acggcgatga gggccagaga ctgcagcaca agggagggga tttgcagccg gggtgccgta
      541 tcgaccgccg gccgcaggga gaatgagagc aggaccgtcg acagggccac cagccagtcg
      601 agtccactgc gggagagggc agcactggcc cggcgtgaga agaagagata aaccagcagg
      661 ctgttgtagc aaaagaggcc aagtcctgtc gggttcgcgc ccccaaacca gtggtgaagg
      721 gaagagatga ccagacccag ccagaacaga gcgagaaaga aattggctgg gccgtgcaag
      781 agctgctgcc aggctgcggc gctggcgtgc gggtgggcat ctacggtcag ttcgaggcgg
      841 tcgccgggct gaagaggggg agcactcccg ccccccccgt cccactccag gacctccgtc
      901 gcgccggcga ccctggggat gacgcggccg ggcgccagcc tctcctcgac gcgcagcacc
      961 ccgccggctt gatcgagaaa ggcgatgccg agggggcggt ccatggacag ggtatgaatc
     1021 ccgctgcagc ggcggatgag caacgccttg ggctccgcct cctccgccag ggcgaggaag
     1081 ccgagcaggc gctggctcca gctctccgct gcaaagagac ggcatggcca gttccctccg
     1141 cagggatgga tcacaattgg cacggctcac cggttggcat gtttttctct cacttttgcc
     1201 ctgacctctc agcggcggat atcatggccc ggacatgcct gcgaaggtct tgaggatgct
     1261 gatgatgccc ggcccgagga tgacgatgaa gagggccggg aagatgaaga ggattagcgg
     1321 gaagagcatc ttgatgccgg ccttggcggc ctgttcttcg gcggactggc gcagcctggt
     1381 gcgcagggaa tcagcctgag cgcgcagggt atcagccagg ctggtgccca gcttgtcggc
     1441 caggaccagg gcgccgacca gaatgcgcag gttctcgacc cggttgcgcg tgctgagatt
     1501 acgcagggcc ttctcgcgtg agagaccggt gcgcatctcc tgggtgacca caagcagctc
     1561 ctggctcaga atcggagcgc gcatgcccaa ctcctgggcg acgcgcagca gagcagcatt
     1621 gagcccgagt ccggcctcca tacagatcac cagcagatcg agagcgtcgg cgaggctctc
     1681 gccgatctgc tgacgccgcc gggagatgcg atggcgtaaa agataatccg gaaagcggaa
     1741 cccgatcaaa agggtcagca tcagcaggac cggagcgagc atgggctggt tgatgtgacg
     1801 gtaagcaaag agataaaagc agaccgcaaa gaggaagagg ctgcaaatgc gcaggcccat
     1861 gagtatgaca gggccatact cgtgatgcag gcccgcctgg atgagcatct ccctgctgcg
     1921 cgagaccgcc ttctcgttgc tgcctccgag gcgccccagg ctgatgagta gcttttcaag
     1981 accagccgga agctcggtct gcttaagggc atcatggatc gtgatctcct cctggaccgg
     2041 agtatgcgcc tcggtcaggc tgcgcacgcg gcgggtggta ggatccagcc tggcgcgggc
     2101 cagtcgcagg atgagcagga tgaggaaaaa gaccgtgata aataccgccg ccagggcgag
     2161 atagagtcgc atgccccccc ctattgatat cggatggcga tgagtctgcg aataaccaga
     2221 aagccgacca cctggaggac aaaaccgatg gtgagcaggg tccggccgaa gcgctccgag
     2281 agcatcggct cgagataggt cggattgatc acctgcaggg cgagcaaggc aaccagggga
     2341 aggctgccaa gcacccacag ggtcatccgg ccctgggcgg tgtaggtgcg gatctgcccc
     2401 atcagtttga agcgctcccg gatggtgtag ctgattttct cgagtatctc agccagattg
     2461 ccgccggtct cgcgctggag aagaacggcg gtcacgaaga gtttgagatc gaggctgttg
     2521 acccgctggg tgagattgac gagggcgtcc cgcaggggca agcccaggtt gttctcctcg
     2581 aaggttttac ggaattcaat gcaaacgggc tccggtgcct cgatggcgac catctgcagg
     2641 gccttggtga gggcgtgtcc tgcccgcagc gaggcggtca tcatatccag ggtatcggga
     2701 aaagcgcgga taaaggcgcg cagccggcgg gtgcgcagga ccaggatata aagcagaggc
     2761 agggcggcag cccccagggt caccaccacg cgcagcagca ctcccggggc gccggaggag
     2821 agcaggaggc cgcatgctcc caatgccgcg ctcgtgagca gaaccatgcc gacattatat
     2881 ttgctgcccg cctgttcgag caggaggcgc agccgggccg gaaaatcgag ccctgacaga
     2941 aggcgattga agagcgagat atcgctcaac cgctcatcgc gcaggacaaa gggcatacgg
     3001 gcgggattga tcccggcctc gacggcatag gcggccagcc ggcggtcgat cttgcgcttg
     3061 ctccccgcct tgcgctcata gaccaccaga agaagggcga gcacaaagaa gaggaagaaa
     3121 agaaacaggg cgctcatcag caggatcatg gggcggattc ctcctccatc ggcagttcga
     3181 agcgcggttc aaacatctca tccggcattc tgatccccgc ggtctcgatc tgctcaatga
     3241 acttgggacg gatgccggtg gtgcggaagg tcccgaccac ctggccatcc tcacggatgc
     3301 cgagcttttc gaagaggaaa atgtcctgca gggagatggt ctccccctcc atgccgacga
     3361 tctcgacgac cttgaccacc ttgcgggagc cgtcgctgag gcgggagatc tggacgatga
     3421 tgtggatcgc agaactgatc tgctcgcgca tggcgcggtc gccgagattg gcgcttgcca
     3481 tcagcaccat ggtttcgagg cggcgcaggg cgtctctcgg cgagttggca tgcagggtgg
     3541 tcagcgagcc gtcgtgaccg gtgttcatgg cctggagcat atccagggct tcggcgccgc
     3601 gcacctcgcc gacgatgatg cggtccggcc gcatgcgcag ggcgttgcgc accagatcgc
     3661 gctgcacgat ggctccgcgc ccctcgatgt tggcggcgcg ggtctcaagg cggacggtgt
     3721 gccgttgctg cagctgcagc tccgcggcat cctcgacggt gatgatgcgc tccccgctgg
     3781 ggatgaagcc cgagaggatg ttgagcaggg tggtcttgcc cgaaccggtg ccgccactga
     3841 cgaggatgtt gaggcgggcg cgcacggcgg cggcgagcag ctcggcaatg gcgggggtga
     3901 ggctgcgcag ccgcagcaga tcatccatgg ccagccgctg cagaccgaac ttgcggatgg
     3961 agaggatcgg accgtcgatg gccagcgggg ggatgatggc atttacgcgc gagccatccg
     4021 gcaggcgggc gtccaccatg ggactcgatt catccacccg ccgccctacc ttggagacga
     4081 tgcgttcgat gatgttgagc agatggacgt catccttgaa tcgggtgttg gtggtctcca
     4141 gcttgccgaa gcgttcgacg tagacctggc tgcaggtgtt gaccaggata tcggagatgg
     4201 tcggatcgtg gaggaggggc tcgagcggac ccaaaccgaa ggtctcgttg aggatctctt
     4261 ctgtcaatcc ctcgcgattg agttcgaagg agacctcctc gtcggcgctg agcaggctct
     4321 cgatgacctc acgcaccagg gtgcgcaggg tctcctgggg gatggtatcc agcttggcaa
     4381 ggtcgagctt ttcgaccagg cggcggtgga tgcgttcctt gaattcatgg taggcgtgct
     4441 ggctctgcag gtcactggag actgcggcaa ctttgcatac ctgtgcggac ggggcgggct
     4501 tcgccggccg agccccgttg cctccttcct caccggcttt gagccgttgc aacaggctca
     4561 cggttcactc ctttcgtcca gtcaggcgcc tgagcagccc cctgctgttt ctgggcgctg
     4621 cggtgttttt tgcccgcccc tccgcctgga tatggtttaa aagttcggcc atctgcaggc
     4681 tgaaaggatg gcggggccgg ctgagaatga tgggctcgcc gacattgatg ctttcgagca
     4741 gggcgccgtt ctcctgcagc ggcaggcggc agcaaacgga tcgtccgatc gacccctcaa
     4801 tgctctgcaa ttcatcgctg atgcgctggg gatagcggtt gaggaccacg gcgatctttt
     4861 cttcggcata tcccatacgg cggaagaggg cgagggtgcg tttgagatta tagactgttg
     4921 agaggtcgag ttgagcgatg gtcaggatca gatccgcctc atccaggacg cggatggtga
     4981 cttcgtcgaa atggtcgtcg caatcgatga taatgagatc atattccggg cgcagcagtt
     5041 cgaggaggcg ccccagaccg gcagcggtaa ttttctccgc ctcttccatg ctctccgggc
     5101 cggcgatgaa gtgcagaccg ctgccgtggc cggggaggat agcgtcgagg cgtgaaacct
     5161 cgagggtggc gaggttttcg agcagttcga gcagggagat gcggggtttg atatcgagaa
     5221 aaagggcggc attgccgagc tggagtttgc tgtcgatgag cagcaccctg cggccattat
     5281 tcttcgccgc ggcgacggca agatttaccg ccagggtggt tgtgcccatt ccccccttga
     5341 caccgaagca gcagacggtt ctggcccggg cgggcctgtc cctgccgctg tcgacgcccg
     5401 gcctgaccga ctttaaggcg cggcttatct cctcatcatc ggcatcggga agcagaaatt
     5461 cacctgcccc gcagcgcatg gcgcggacga tcagttcagg ccgggcctgg cctgccgtac
     5521 agaaaaagcg gatccgcgga aagagcgcac tccatcgcgc caggtagtcc agcccctcct
     5581 ccccggtgcc gaggtcgagg agggcaatat ccggctgcaa cctggtgagc agagcctctc
     5641 cctgcagcca ctccccgcac tgcccgatca ggcgcccccc ctcctgtttc tgcagggcat
     5701 caaagacggt atcgggatcg ttctcgctga tgtagatcca tgagaattct tttgccatag
     5761 cgcccgtttc tgccaatcat tccaccaggt gtacgccgta gaggtaggtg ttgccgttgc
     5821 cccagatacc gtgggtcagc atttcgataa agcggccgta gagagccttg ccctgcatcc
     5881 cctcgataaa aaagaccccc aggccggtca ctgtaacggt gttgcggccc ggatccgggg
     5941 ggtagcgctc gtcatagaaa gggacgatgc agatacgcgg actcgaaaaa ccgggatagc
     6001 tggagccctg cacggagttg gtattattgt cccaataggc gccggaatcc caacgcagca
     6061 gagcctccac cccgtgtttg gtcgggccca ccatgttgcc cggctccacc tggatgatat
     6121 cgccgatttc gacaatgccg tcgcaaccgc tctcaatatt ctcactgtag gcggcaccgc
     6181 ccacctccgg ggtgccgcgg ttgaccggcg gaaaatcgac aggataaaaa aagcccggat
     6241 tggtgccggg agcgtccagg gaaccggcct tgagcagggc atattctccg acccggtaat
     6301 tcagatcggg gacagcaaag ggcctgaggt ggtcggtgcc gatgatcgag cccaactccg
     6361 cagtcgctgt agccgagatc gtcacccgcg ggatgcccaa agcggacgcg aaaaagaggg
     6421 gcagctggcg ggaggatgtg acccggatcc ggctagagtt gggaaagctg atatccccgg
     6481 cgccgaccgc caccggctgg ttgatgcagc ggttgcggcc ggcgaaatcc atcgcctgct
     6541 ggatggccag gttctggctg gtcatcaggc cggcggcccc ggccagcgca gaggcatcca
     6601 ccccagcctg cagctgattg cgcgcggtga agagatagcc cgcgtcaatc gccagcgcgg
     6661 ccatacccat caccgccagc atcgagaccg cagccaggac tagaatggcc ccccgcgtct
     6721 gacgcacaag acgaatcgca accttggccg tcatcatggc ttgattcctt catcaggcat
     6781 ctcagcgctt ccggtggtga tccggggcga gaccaggatc atcagctcac tctctttctt
     6841 tttaaaactc tcactgctga agaggcggcc cagcagcggc acagcgccca gcaggggcac
     6901 ccgggagagg gttcgcgcct cttcatcagc caaaagaccg ccgatgacga ggtagtggtt
     6961 ttctttcagg tccacattgg tctcggcctt gcgcgtggcc agggcgggga tcttgaagcc
     7021 ggacagggtg ataccgttgt caaaatcgag gctgctcacc tcggcggcca ccttgaggtt
     7081 gatcatgctc gaatccatga catggggagt gaatttgagc ttgatcccga actccttgaa
     7141 ctgaatggtc accgtctgca tccccaccga gccagagacg atggggatcg ggaattcacc
     7201 gccggcgaga aagctggcct ccgccccctc gcgggcactg agattgggtc tggccaggat
     7261 ggtgatgaga tttttttcct gcagggcctt gatcatcacg gtcagattgc gtgtgggcac
     7321 cgcgaaaagg agatcgaccg cctcattgag cccaaggggg tcgttcacag aggccgcctg
     7381 cccggcgaaa ctgccgacat cgatgcgctc gtcaccggcc ttatgatcct tgagcagaaa
     7441 atcgaggccg agctcgcgca aggcgatgcg gttgacctca acaaaacgaa cctgcagcat
     7501 cacctgatgg agggccatcg gccggcgcac cagcacgcgc ctctgctcgt agcgcccctg
     7561 ctcatcccag acaatcaggc tggtggagcc ctccgacttg ccgatcagca ccagctgtga
     7621 ggccgagatg acattggcat cggccacctc cggatcggcg atcgagacct tggcaatgcc
     7681 ccggctgtac tccatgacct gggagtggcc gggttccagg atgagcatgt cggattccgc
     7741 cgcagccccg gttaaaaatc caaggagaag ggcgatcgct ccccaatgcc agatccttga
     7801 cgataccagg atgcgtttct tcctcaccat tctgcagaac tcctttccgg tccagcggaa
     7861 tctcacttca ggggtttctt ctctgtcgcc ttgccctctt ccagcaccac ctctgatcgg
     7921 gtgctggagc gatagacctc gatgacctgt ttgggcggct ccttggccgg ctcctcgcgg
     7981 acggcgggcg cagcggccgc cctgcgcacg gctgcggcgg gtgtggtggg ccgcgcatcc
     8041 ttgttgatca gctccttcag gctcaccccc tccgtcaccg cggtctgctg atcggcggta
     8101 ttgcggagaa ccagctgcag cttgccctct gtgctggcca gagcgagctt ttcggcctgg
     8161 gccgggttga ccagcagggt gaccgacttg actgtaatcg gatcatcatc cttggggctg
     8221 acggtctggt cgacggcaag gacctcgata ttttcgagga tggtggatgt tttcgactcc
     8281 tccttgtcga gggtggccga aaccgtggcg aggacatcga cgcgcgcatg cgggagaatg
     8341 aagccgctga cgccgctgac gatgttgacc gcgacggtca tggcacgcat gccggggggg
     8401 ataaccgccg aaacaccacc gctggagccc ggcggggcca ggcgtgaggc aaggagggcc
     8461 tcgccggcga agatttcgga tttgacaatg cgcccggcga gttcggaggg ttgggtgaag
     8521 ctgccctctg gcgcaagcgc ggccgggaaa agcctggcct caagaaggct ctcctccagt
     8581 tttgagccgg caggcaggtc gattttggcg atcacgaccg gccgcatcgc ggcatggttc
     8641 tgctcgagtg tttgtttctg gttttgcaga taggagtaag cccccagggt cgccagcagg
     8701 gcagtgacca tcgccagggg gatgatgaac ttgagcttga gcagaaacat cgcgcgatct
     8761 ccggtttagc cttcccagtg catggtggtg gtgcggctga gcggaatggg gccgatcaag
     8821 ccgggaatga tggcgttggt caggggaacg taggtgccgc tgacggtgac ggtcacttca
     8881 ttggagccgc cggggccgct gaccgagatc gagaggccgg tgagggtgac cccgccgagc
     8941 agtctgagcg ctgcggcgcg gctgcggccg acgtccgggg ctgtgacagc tgctacgcgt
     9001 gctccctcgc gcgccgctcc tgtcatggta ttcatagtct cccagatccg cccgaattcg
     9061 atgatgccga agaggagcag taggaaaatg gggatgacca gggcgaattc gaccagggac
     9121 tgcccctcct ggcggcggac aaaagaaatc atggggagat tcctcgtact acagtccaaa
     9181 gtgtccccgc ggcgatggcg atgccgtagg gcagcctgat ttcacgtaaa ggcagggggt
     9241 ggcccgaggg atcgcggagc gatctggccc gatgccatgc catggcgcag agcgcgatga
     9301 taccgccggc ggcgacagcg gaaaaaagcg ccgccaacgc agccggcatc gccagaaggg
     9361 ccccggccgc agccatgagt ttgacatctc cagcccccat accgcccaga aggtagacca
     9421 ggccgaagat gagcagcgcg cacgcggccc cgcccaggga ggctgtgaaa ccctgcacgc
     9481 cgtggctgaa ggtatggaac aggaggcccg acgccagggc gggcagggtg agccagttcg
     9541 gaatccggcg gctgcgccaa tcccagacgg ccgccaacac gaccagcaaa atgaccacgg
     9601 cgatgtgcgc tttcacaaca atactgcctt ttcagttgca tagcagggag atggggaccg
     9661 ggcgctcgcg catccgttag tcttctgctg tcgaagagcg ccgggatcaa aggggacacg
     9721 gctgaagccg gctgctgatc ccgcaacctc aagcttcatt tacggtgttg gatgaccgac
     9781 gccggtcccc cccgaaggac ggttgtaata aaaatggcgg tgttagagcg cgccgatgat
     9841 ttgttgaaag ataccggaga tgctgacacc cagtgcgccc agtacagcga cagcagcgat
     9901 ggcgatcagg aagagaatca gggcgtattc ggccagggtc tgtccctctt ctctgacgaa
     9961 aagggagcgg aagatgttga tgaggatctg cattttatgc tcctggaatt agtgtccggt
    10021 tgattaaggt caacaatagt acgctcttca ggctggctgc ggtttcctcc tttctggtca
    10081 aaatgggatg gtccatcgcc ccccggacaa gacaaggtca ggccggttat ggaaaaacgg
    10141 cgaggatctg gttgaaaaaa gcgacgagct gcccgccgaa taaggccacg actgcagccg
    10201 cagccaccat aatcaacagc aggatcagcg catattcagc gatcgtctgc cccccctcat
    10261 catccccgaa ctcatataaa agatggattg acgcacgcat tatcgtcccc cgctgagaac
    10321 agaaacaata atgatgcaga tgtgaaaaac cccgggaaat aataaaaaag acgttctgag
    10381 aagggcatgg tgcggtgcca ttctgacaga acgtctaccc ccccagtgta aagaatataa
    10441 agagaggaac cggccaaatc tgcatgtgcg aatgaagccg ctttgttacg gctgattcat
    10501 cagaaaggac tctccgggga ggagagtggc ctttccagag ccatccgggc ccttgggccg
    10561 ctgatgcctg cacgttcccc ctgtgccggt tcccctcttt gtaaacgtaa aatggccatt
    10621 cggccatctc ttcatttcag agaacagcat cgtaggaatt tgaatagaca gcgtgtgccg
    10681 gttggaatgg aagagcggaa aacccctgga gtaccgaaag accgtctcaa ggctgcctgg
    10741 ccatgatcaa gaacgattct catcttctcg agccggttcg agtgcctccg ctcagggatg
    10801 agcggacccc acctgcgcca aagactggcg ccaactacca ctattcatag tataatcaat
    10861 ggacatatca atgtcaacaa cttttttatg tttttttacg aaaatgcgct attattatac
    10921 acaccacgca cctttctcat gggtaatgtg gctcttgtgt cccataagag tgcagatccg
    10981 cctgctcaga aacagctcat gccgctgtga cgaaatcaca aagcgaaacc aaaaattcgc
    11041 ttgcatcctt gctttaaaag tgtaaattac tgcgctgaat ttcatcaaac aagagggatt
    11101 atgatcaaaa tcaaggctct gcgcggattc aggccgcgcg atgacatcgc cgcccgggtt
    11161 gctgcgcctc cctacgatgt catctcctcc gaggaggcgc gcgagatggc caataacaat
    11221 cctctttcct tcctccatgt taacaaaccc gaaatcgatc ttcctctatc caccgatctc
    11281 tactccgatc aggtctatgc caggggagcg gagaatctgc gccgtttcat cgccgagggc
    11341 ctcctccggc aggaccctgc cccctccatt tacgtctacc agcagcagtg gcaggggcgt
    11401 gcccagacag gctacttttg tctggcctcg gtcgacgatt acgatgccgg ccgcatccgt
    11461 aaacacgaac tgacacgggc gagcaaggaa aaggaccgca ccaccctcat ggaccgccag
    11521 aacgcccaga tcggcccggt ttttcttatg tacgaggccg aaccgatgct ggatggctgt
    11581 cttgccgaga ccatcaccac ctcccccctg atcgactttg acaccccgga gggtgtccgc
    11641 catcttctct ggcgcatcga cgacactgcc cgcatcgccc gaattgagcg cggtttcgca
    11701 ggcctcgaca agctctatgt ggccgacggc catcaccgct ccgccgccgc cagcaacctc
    11761 tgccgcgaac ggcgtgcagc caacccgaac cacaccggcg aagaacccta caactatttc
    11821 ctcgccgtga tctttcccca cacccatctg agaattctgc cctacaaccg cgtcgtcacc
    11881 gacctcaaca gtctcacacc ctctgaattc ctggccgccg tcgaggccaa atttactgtc
    11941 acgccagccg gaccggccgc tccagaggtg cccctgcatg ccatggggat gtacctcgaa
    12001 ggcctctggt atttgattgc gcccaggcct gggacctggg acagcagcga cgccatcgag
    12061 gacctggatg tcaacatcct catgaagaac ctgctcgtgc cgctgctcgc tatcggcgat
    12121 ccgcgcaccg accagcgcat cgactttatc ggcggtatcc gcggagctga ggagctggtc
    12181 aggctggtcg attccggccg cttcaaggtc gccttcgcca tgcacccgac ctcgatcgag
    12241 caactcatgg ccgtggccga tgcgggcaag atcatgccgc ccaaatccac ctggttcgag
    12301 cccaagctgc gcagtggact ggtcattcac ctgctggatt aaaccagaca agaggagctg
    12361 atgatgaaaa tcgtcttatg tgacaaattc gacgccaccc tgcccggccg tctcgccccc
    12421 tacggcgagg tgagtgatga caagaatctc attcccgagg ccgatgtcgt gctgatccgc
    12481 agcaaaacca aatgcacccg ggagttcatc gacggcgccc ctaacctcaa actgatcatc
    12541 cgcggcgggg tcggcatgga caacgtcgac aaggcctatg ccaaaaccaa aagcatcaag
    12601 gcctacaaca cccccaacgc ctccagcatc gcggtcgcgg aactcgcctt tgccctcatg
    12661 atcgccatgc ccaacaaact gatcacggcc cacaatacca ccgccgccgg gcagtttctc
    12721 aagagcgaac tcaagaggac cgaactgctc aataaaaccc tgggcatcat cggctgcggc
    12781 cgcatcgggc tggagttggc caaacgcgcc cgcgcctttg gcatgaccgt ctttgcctac
    12841 gacgtcgtcc cgctccagag cgagcatatc catggccaga aatcgcttga ggagcttctt
    12901 cccctctgcg attatttttc cctccacctg ccgctgactc cagaaaccac cgccatgatc
    12961 aacaaggagc gcatcgccct gatgaagcgg ggcgcctatc ttgtcaacac cggccgcggc
    13021 aagaccgtgg tggaggcgga catcgtcgag gccctgcaga gcggacagct tgccggctac
    13081 ggcaccgacg tcttttatgc cgaaccgccg gagggttccc ccctgctcaa cgcccccaat
    13141 gtcgtcctga cgccccatct gggcgcctcg accgcggaga acctgctccg catcgccgat
    13201 gaagtggtga tcctgatcga caaatttgcc aaaggtcaac tggactgata gaggttcaac
    13261 caaaaggagt caaatatggg acgcatctat aatttcaatc cggggcccag caccctgccc
    13321 cttgccgctc ttgagaaggc ccaggcggag ttcaccgact acaagggctc gggcatgtca
    13381 gtcctcgaga tctcgcaccg ctccaaggat tttgaggcga tcctcgccga cacccagaac
    13441 ctgctcaagg agatgctcgg cattcccgat aattacagca tcctctttct cggcggcggc
    13501 gccagtctgc agtttgcgat gctggcgatg aatttcatcc cggccggcgg ctctgccg
//
LOCUS       DAOPWU010000057        13548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4644_length_13548_cov_4.316238, whole genome shotgun sequence.
ACCESSION   DAOPWU010000057 DAOPWU010000000
VERSION     DAOPWU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13548
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4644_length_13548_cov_4.316238"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKM23_04855"
     CDS             <1..256
                     /locus_tag="PKM23_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX subfamily"
                     /protein_id="HNY90835.1"
                     /translation="ILIGLGLIMGLFTRIAAGGGILLILLYYVCNPPFVGLFYSIPME
                     GHYLFVNKNLVELGALLVLIAIPSQQVAGLDRILYKLIKK"
     gene            281..955
                     /locus_tag="PKM23_04860"
     CDS             281..955
                     /locus_tag="PKM23_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90836.1"
                     /translation="MSALKKVLIPFVLLTLALLVLSLSAQGLKAINIKLPKPMFVGTP
                     QNVKVPNLEKPLGKPRDAFLAPAGTVNVALNKPVTSSDMQPVIGELALVTDGDKEAED
                     GSYVELGPFVQHVTIDLKSKHSIYAVVLWHYHQQPVVYFDVTVQVSDDPSFKNGVQTI
                     FNNDHDNSARMGAGKDKNYIETAEGKLIDAKGVQGRYVRCLSNGNSGNDLNHYIEVEV
                     YGLPAK"
     gene            1058..2632
                     /locus_tag="PKM23_04865"
     CDS             1058..2632
                     /locus_tag="PKM23_04865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNY90837.1"
                     /translation="MRNHRLLLILWVLVFLLGALLRLPRLEQRPMHTDEAVHAVKFAA
                     LLQEGSYTYDADEYHGPTLYYFTLLPAWLRGERTFAELSAATLRSVPLFFGLGLILLP
                     LLLTPFTGRWAALAAGLWTALSPMQIFYSRYYIQENLFVFAGFLTLMLGARYFSRRHW
                     GWAAGAGAALGLLHATKETDILLVAAAVLAGGVLWLRQRGRLRVEPLHLLIFLAAALL
                     VSALLLTAGFNHLQAFADSFTAYSGYLRRGAGNSLHLQPWYHYLRLLFFFQEPGRPLW
                     SEAWLLPFVVSGCVLIARAFLQKEDSRRPAVLLFIALFTAALWLIYAVIPYKTPWNGL
                     AAHYGLLLIAGVGTAGLLSSMRNRTAGILLLACCALPLGIQAHLLNTTYASDPGNPWV
                     YAHPGADLQRISILVEAAAGAGPDSLLTPVEIIATGDQYWPLPWSLRRLLNIGWYSRV
                     DTTLPAAPIILCTPDQEQLLLRKLYEIPPAGERSLYLPLWREYAELRPGVEIRGYIRK
                     EFIDASGGGGSRAAAQ"
     gene            2616..3548
                     /locus_tag="PKM23_04870"
     CDS             2616..3548
                     /locus_tag="PKM23_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNY90838.1"
                     /translation="MQRRSEDLLHPLVEHPLADARIHRGSHQAMATEWAIWISHEDEV
                     YARQAAWEAFQLLERLEAELSRFNPNSDIARLNRLTPGEILPLGAEAFGCLRKARALF
                     EASGGAFDVTAGGLKDHYRENDSLRGRIAGRLRASRQPVGMEHLHLEGETMRVWRDVG
                     VEVDLGAIGKGYAVDRMVELLQEWGLSDFLVHGGASSVAARGHAPAHSGWPVTLRHPR
                     DGGRVQAQFPLTEQALGASGIARGEHIIDPRRRRPARTPLAVWVAASSATEADGWSTA
                     FMVMSGTAIRSCIGEKQVTRALILGRDYSLTALP"
     gene            complement(3601..6792)
                     /locus_tag="PKM23_04875"
     CDS             complement(3601..6792)
                     /locus_tag="PKM23_04875"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNY90839.1"
                     /translation="MPFAASRLMRLITPVLLAAVSILAAAPGQSIPLANPGFETLQAE
                     GAAGWQPDSLSTACISLDRAVIRSGQSSLHILHASPRRSTLLSQPVRLEVGKLYRLSG
                     WIRTSGARTDSAQHYPTPLPACLRMGGMPITAWSRPIGGDQEWQLSEALFFASRAEDR
                     VELRLGYRGDAAGEAWFDDIAITPVDDITEYIPAERIRWYGKAFRFEEQGWTYLHIEG
                     SAYERGFQHGALLARELKTFITKLATSRNRDNPAAGWRDLRSQVDLLFLRKYETEFME
                     EMQGIADGAKKEGATLFDRPVELLDIVTINSYIDLSYAWDAVQNTPHQLSGQRFKAPE
                     IDMDLPERLHKCSSFLATASATADQRIVFGQLFMWNGYMGTEWDVFIDLVPEKGERLV
                     YETYPGGIHSGADFYINQAGIMIGETTVGQTPWNPDGTPQSNRIRLAAQYARSIDDVV
                     KLLWNRNNGMYTNDWLIADCKNDEIAIYCLGTHAAKLWRSTKREFYDGQIDWYWSDNN
                     AKSMDIRKEYIVNSDNAPFDLSFRPWDRDLAFHKFYQQNRGRIDARIGMELIGSSPTN
                     RPHACDGKITTSEMAEKMMFMAHYGKVTYREMFINENNRIPDLPLATPRLSLGYTAFS
                     PLTLGAMIGGMKKPQSPVNKAEPRPETAEVDSLYRFPAELLWGNTVFPASTAENWLIS
                     ATAAYWQMLHALPKEAAKARAQLVNDLTELNCRLLHTEAREGALVPAQAQQIYHQMKQ
                     YYIPRNRGTFLLHQLRLRLGNAAFARLMNRLHDEYAGKEVTSARFREMAEEAGGEAGR
                     LIDEWVNRDDLPDCQPECQVTGADSLWKVRFTVEQKGKPFHLVSAVQIRTAGSCIWKK
                     IELKEARQEWNWEVDEKPIEITLNPGNDFPVRQDRYYTFSNFYEDWSHTLIVYGASRQ
                     IEANHTLALRFAKAAADRITESLPPVRKDCEFDLADFREHDIILLGGPADNRWVREIA
                     PVCGLDLGNGIFAWQGKSHADANEGLVVVLPNPHNSQRMVTLIAANSALQLYQMTKAL
                     PRFPQWALFRDDQIVERGYHEPGLEAKMEVMLP"
     gene            7082..8491
                     /locus_tag="PKM23_04880"
     CDS             7082..8491
                     /locus_tag="PKM23_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HNY90840.1"
                     /translation="MASSSIAKPVEPQSGGEEKRYGGPFAIMTVLFFMWGFMTVWNDL
                     LIPRFKEAFTLNYFQAMLVQFAFFGAYTIGALIYYLFSTVSGDPINRIGYKNGVIIGL
                     ITSAVGSALFYPAAVMVSYTFFLVALFIVGLGFAMLQIAANPYVTILGPEHTASSRLN
                     LSQAFNSLGTTIGPVIGGYLIFKVFNQPGAHGADAVKTPYLLFAGVFLLLALVFRFIH
                     LPNFSNAEQVSHGAGALKHPHTVLGMIAIFMYVGGEVAVGSAIINFLGTPRLGGLTHE
                     TASRYLSFYWGGLMIGRFMGAFALSDMAKSRKRLLVAVIPVIAFFVILALAGPAEALR
                     YGLFLLILLAAFYLGENSAHRMLALFSGVIIVLLLTGMTASGVTAQWTILSVGLFCSV
                     MWSNIFSLAIEGLGALKSQGSSLLVMMILGGAILPPLQGWIADMAGIQFSFIVPMIAF
                     FYVAFYGLYGYRAGRNLQA"
     gene            complement(8506..9270)
                     /locus_tag="PKM23_04885"
     CDS             complement(8506..9270)
                     /locus_tag="PKM23_04885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90841.1"
                     /translation="MAAAADDAPAVDLSIIIPVYNEHGKIAGDIRAAADFCARHGLKG
                     EIIVADDGSDDGTLEAAEKARPTAGAAELLPLRLTPHRGKGHAVREGMVRSRGRRILF
                     IDSGLCIPYDEISRGLAMIQSGACTIAHASRRLPTSRILKPQSPSRRISAWLFRRLFT
                     RLLGLPQELTDTQAGLKIYAGESGRALYRSCSTDGFLFDAEIILRAMHGGEKICEFPV
                     SWSADPDSRLQLRRMPLALLREACALKKRLRREFAG"
     gene            complement(9277..10710)
                     /locus_tag="PKM23_04890"
     CDS             complement(9277..10710)
                     /locus_tag="PKM23_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNY90842.1"
                     /translation="MTDHLLKICLVLFLGLAVMPVRGQEAAGPYKDVRIKKMKMAMQT
                     YTFRKYTFAEALAKTRELGIRYVEAYPGQRLSKEMPANVLFSHTMSDEQIAWVKDELA
                     KNSLTLVAYGVVGFDSTEKAMREVFDFARKMGIQVINTEPAFTDWRPLERMVKEYNIK
                     VAIHNHPLPSRFALPATVLAKVKGLDPRIGSGMDTAHWMRSGWEPLKAMALLRGRIVH
                     AHLKDLDLFNDAHQAKDVPFGSGVAKIHDILAELTLQDYDGYLSIEHEREDESSNPSP
                     NIAKGLEYIRSITYFQDYQQLLPRWDGRYSKHGWNHYGPGHFLLDEKTGELTGQGGMG
                     LLWYSARKYRDFILELDYKCDEIFTNSGIFLRVPEMVTSDDYIYKSFEIQIDDAGTGI
                     HQTGAAYDAEAPKTLAFKETGEWNHYKISFIGGTITVELNGVEVVNWRAEPRGKIKSY
                     TPEGYIGLQNHDSRAPIHFKNIFVKEI"
     gene            complement(10755..12002)
                     /locus_tag="PKM23_04895"
     CDS             complement(10755..12002)
                     /locus_tag="PKM23_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY90843.1"
                     /translation="MAKKVLGVGFVGGGFVAQFHIRAWVSVRNADINGIVAKSPAEAE
                     QAAALVRQLNLGDGKIYKSVTEMVADPAIDAIWICAPNFTRVEIMEEIAEAVISGKGQ
                     LIGVACEKPLGRNVREARRMVELAQKAGLLDGYLENQVFSPTVIRGRDIVWLRGAAAT
                     GRPYLARASEEHSGPHMPWFWEGELQGGGVLNDMMCHSVETARYLLTPPGAPREALTP
                     VKMSAYTNCLKWQRPDYAKHLAEYSKGKLDYTNRPSEDFARSLIEYHDREGNQVVVET
                     TTSWCYVGAGLRLSMELLGPEYSMQVNTLDTGLKVFFSREVAGRSGEDLVEKQNAEMG
                     LMPVVSNEGNEYGYDVEDRHMVDCFLAGVRPSETFEDGLEVTTLLMSAYMSAEQEKTI
                     LLPAPGIEDFIPAVARGAWNPRK"
     gene            complement(12110..13321)
                     /locus_tag="PKM23_04900"
     CDS             complement(12110..13321)
                     /locus_tag="PKM23_04900"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY90844.1"
                     /translation="MSVKVRLGIMMFLQYAIWGAWSPVLSAYLENTLHFSGMQIGVIY
                     SLLPLATIIAPFIGGQFADRYFSSEKVIAVLQLIGGGLLIYISRLTDYTAMMWLFFLY
                     SLLYAPTMALTNSIAFINMKDSEKEFGQVRVWGTIGWIVAGLLLTGWRYLGSAPGADT
                     LLLAGIFSLIMGLQSFTLPHTPPQKNAAKPWAFIEALKMLKDRNVLIFTIIAFVVATE
                     LMFYYVLTAPFLESSIIGVAKENTPMVMVIAQAAEILVLAFLLPWALPKYGIRTVLTI
                     GILAWPLRYIIFAIGSPAWLVIASLSLHGFCFVFFFAAAFIYFDTIAPSDIRNSAQSF
                     ITLVTYGLGNYVGSLFAGWIKSMFTTSQGVTNWTNVFLVPCALTILCAIAFLAFFRVD
                     TRRNAAKTVKA"
BASE COUNT         2437 a         4111 c         4228 g         2772 t
ORIGIN      
        1 catcctcatc ggcctcgggc tgattatggg cctcttcacc cgcatcgcgg cagggggcgg
       61 gattctcctc atcctgctct attacgtctg caacccccct ttcgtcgggc tcttctattc
      121 catcccgatg gaggggcact atctcttcgt caacaagaat ctggtcgaac tcggggccct
      181 gctggtcctg atcgccatcc ccagccagca ggttgcgggg ctggaccgga tcttgtacaa
      241 actgattaaa aaatagcctc tctagatgga gattcctgca atgtccgctt tgaaaaaggt
      301 gctgatcccg ttcgtcttgc ttacgctcgc cctcctggtc ctctccctct ccgcccaggg
      361 gctgaaggcg atcaacatca agctgcccaa gccgatgttc gtcggcaccc cgcagaacgt
      421 caaggtgccg aacctggaaa agcccctcgg caagccgcgc gatgcctttc tcgcccctgc
      481 gggcacggtc aatgtcgccc tcaacaagcc ggtaacctcc agcgacatgc agccggtcat
      541 cggcgagctc gctctggtca ccgacgggga caaggaggcc gaggacggca gctatgtcga
      601 gctggggccc ttcgtccagc atgtcaccat cgacctgaag agcaagcact ccatctacgc
      661 cgttgtgctc tggcactacc accagcagcc ggtggtttat tttgatgtca ccgtccaggt
      721 ctccgacgat ccctccttca agaacggcgt ccagaccatc ttcaacaacg accatgacaa
      781 ctccgcacgc atgggggcgg gcaaggacaa gaattacatc gaaaccgccg agggcaagct
      841 catcgatgcc aagggggtgc agggccgcta cgtccgctgc ctcagcaacg gcaacagcgg
      901 caatgatcta aaccactaca ttgaggtgga ggtttacggc ctgccggcga aataggcgcg
      961 cggaggtttt accgaccaag ccgtgatgcc gccctcattt caacatctgc acagaatgcc
     1021 cctccagccg ctggaggggc ttttaatttg gagccccatg cggaaccatc gactccttct
     1081 catcctctgg gtccttgttt tcctgctcgg cgccctgctc aggctgccgc ggctggagca
     1141 gcggccaatg cacaccgatg aagccgtgca cgccgtcaag ttcgccgccc ttttgcagga
     1201 gggaagctat acgtatgatg cagacgaata ccatggcccc accctttatt acttcaccct
     1261 tcttccggcc tggctgcgcg gcgagcgcac cttcgccgag ctgagtgcgg ccaccctgcg
     1321 cagcgtgccg cttttcttcg gactcgggct catcctgctg cccctgctcc tcacgccctt
     1381 caccggccgc tgggcggcac tggccgcggg actgtggacc gccctctcgc ccatgcaaat
     1441 cttttacagc cgctactaca tccaggagaa cctttttgtc ttcgccggct ttctcaccct
     1501 gatgctgggg gcgcgttatt tttcccggcg ccactggggc tgggcggccg gagccggcgc
     1561 cgctctcgga ctgctccatg ccaccaagga gaccgatatc ctcctggtcg ccgcggccgt
     1621 gctggccgga ggagtgctct ggctgcgcca gcgcggccgg ctccgagtcg agccgctgca
     1681 ccttttgatc ttcctggccg cggcgcttct ggtttccgct ctcttgctca ccgccggttt
     1741 caaccacttg caggcatttg ccgacagctt caccgcctac agcggctatc tgcgccgtgg
     1801 cgccggcaac agcctccacc tgcagccctg gtatcactat ctgcgcctgc tcttcttctt
     1861 tcaggagccg ggccggcccc tctggagcga ggcctggctt ctgccctttg ttgtttcggg
     1921 atgcgtgctg atcgcgcgcg ccttcctgca gaaggaggac tcgcgacgcc ctgctgtact
     1981 gctttttatt gcgttattca ctgcagctct gtggctcatc tacgccgtga tcccctataa
     2041 aacgccctgg aatggtctcg ccgctcacta tggcctgctc ctcatcgccg gagtcggcac
     2101 cgccgggctc ctctcttcga tgcgcaaccg gacagcaggg atcctcctgc tcgcctgctg
     2161 cgccctgccg ctgggcattc aggcccatct gctcaacacc acctatgcca gcgaccccgg
     2221 caatccctgg gtctatgctc atccgggtgc cgatcttcag cgcatctcaa tcctggttga
     2281 ggcggccgcc ggagccggtc ccgacagtct gctcacgccg gtggagatca tcgccaccgg
     2341 agaccagtac tggccgctgc cctggagcct gcgccgcctg ctcaatatcg gctggtacag
     2401 tcgggtggat accaccctgc cggcggcgcc gatcatcctc tgcaccccgg accaggaaca
     2461 gctcctgctg cgcaagctct acgagatccc cccggcaggg gagcggtccc tctatctgcc
     2521 cctctggcgg gagtatgccg agctgcgccc gggcgtggag atccgcggct atatccgcaa
     2581 ggagttcatc gacgcctccg gcggcggagg atcccgtgca gcggcgcagt gaggatctac
     2641 tgcatcccct ggtcgaacat cccctcgcag atgcccgtat ccaccgcggc agccaccagg
     2701 cgatggcgac cgaatgggcg atctggatca gccacgagga tgaggtttat gcccgccagg
     2761 cggcctggga ggcctttcaa ctgctcgaac gtctagaggc agagctgagc cgcttcaatc
     2821 ccaacagcga catcgcccgc ctcaatcgac tcactcctgg cgagatcctg cccctcggag
     2881 ccgaggcctt tggctgtctg cggaaggccc gtgccctctt cgaagccagc ggcggagcct
     2941 ttgatgtcac cgccggcggc ctcaaggatc attacaggga gaatgactca ctgcgcgggc
     3001 gaatcgccgg gcgcttgaga gcctcccggc agccggtcgg gatggaacat ctgcatctgg
     3061 agggtgaaac catgagggtt tggcgggatg tcggggtgga ggtcgatctg ggtgcgatcg
     3121 gcaaaggcta cgccgtcgac cgcatggtgg agctgctgca ggagtgggga ttgagcgatt
     3181 ttctcgttca cggcggggcc agctccgtgg ctgcgcgcgg ccacgccccg gcccactccg
     3241 gctggccggt gaccctgcgc catccccgcg acggcgggag ggtgcaggcc caatttcctc
     3301 tcaccgaaca ggccctgggt gcgtcgggca tcgccagggg agagcacatt atcgatccgc
     3361 gccggcgccg gccagcccgc accccccttg ctgtctgggt ggctgcatcg tccgcgacgg
     3421 aggccgacgg ctggtcgacc gctttcatgg tcatgtccgg cacagcgatc cggagctgta
     3481 tcggcgaaaa acaggtgacg cgggccttga tcctcggccg ggactatagc ctgaccgccc
     3541 tgccctgacg cctagcagca aggtcagacc ggagcgcccg ggagcgcacc ggtccatggt
     3601 ctatggcagc ataacctcca tcttcgcctc gagtcccggc tcatgatagc ctcgttcgac
     3661 aatctggtcg tcgcggaaga gcgcccactg cgggaaacgc ggcagggctt tggtcatctg
     3721 gtagagctgg agggcgctgt tggcggcgat cagggtgacc atgcgctgcg aattgtgggg
     3781 attgggcagt accactacca gcccctcgtt ggcatccgca tggcttttac cctgccaggc
     3841 aaagatcccg ttgcccagat ccagcccgca caccggggcg atctcccgca cccagcggtt
     3901 gtcggccggc ccgcccagca gaatgatatc gtgttccctg aaatcggcaa ggtcgaattc
     3961 gcagtccttg cgcaccggcg gcaggctctc ggtgatgcgg tccgcggccg ccttggcgaa
     4021 acgcaaggcg agggtatggt tggcctcaat ctggcggctc gctccataga caatgagggt
     4081 gtggctccag tcctcataaa aattcgaaaa ggtgtaatag cgatcttgcc gcacggggaa
     4141 atcattcccc gggttaaggg tgatctcgat gggcttttca tccacttccc agttccactc
     4201 ctgccgcgcc tccttaagtt cgatcttttt ccagatgcag cttccggcag tcctgatctg
     4261 aaccgcgctg accaggtgga agggcttgcc cttctgctcg accgtaaagc gcaccttcca
     4321 caggctgtcg gccccggtga cctggcattc cggctgacag tcgggcagat cgtcgcggtt
     4381 gacccattca tcgatcagac ggccggcttc cccgccggcc tcttcggcca tctccctgaa
     4441 gcgggcactg gtgacctcct tgccggcgta ttcgtcgtgg aggcggttca tcagccgcgc
     4501 aaaggcggcg ttgccgaggc ggaggcggag ctgatggagc aggaaagtcc cgcggtttct
     4561 tggtatatag tattgtttca tttgatgata tatctgctgc gcctgtgcgg ggacgagggc
     4621 gccttcacgg gcctcggtgt ggagcagccg gcagttgagt tcggtcaggt cgttgaccag
     4681 ctgggcgcgc gctttggcag cctccttcgg cagggcatgg agcatctgcc aataggctgc
     4741 cgtggcgctg atcagccagt tctcggctgt gctggcggga aagaccgtgt tgccccagag
     4801 cagctcggcg ggaaagcggt agagggaatc gacctccgcc gtttctggtc tgggctcggc
     4861 cttgtttacg ggcgattggg gctttttcat cccgccgatc atcgccccga gggtgagggg
     4921 tgaaaaggca gtgtagccga ggctgagacg cggggtggcc aggggcagat cggggatgcg
     4981 gttgttctcg ttgatgaaca tctcgcggta ggtcaccttg ccgtaatggg ccatgaacat
     5041 catcttttcg gccatttccg aggtggtgat cttgccgtcg caggcgtgcg gccggttggt
     5101 gggcgaggag ccgatcagct ccatgccgat gcgggcatcg atgcggccgc gattctgctg
     5161 gtagaatttg tggaaggcga gatcgcggtc ccaggggcgg aagctgaggt cgaagggggc
     5221 attgtcgctg ttgacgatgt attccttgcg gatatccatg gatttggcgt tgttgtcgct
     5281 ccagtaccag tcgatctggc cgtcataaaa ctcgcgtttg gtgctgcgcc acagttttgc
     5341 cgcatgggtg ccaaggcagt aaatggcgat ttcatcattc ttgcagtcgg cgatgagcca
     5401 gtcgttggta tacatgccat tgttgcgatt ccagagcagt ttgacgacat cgtcaattga
     5461 gcgggcatac tgggcggcga ggcggatgcg gttggactgg ggggtgccat cgggattcca
     5521 gggggtctgg ccgacggtgg tctcgccgat catgatgccg gcctgattaa tatagaaatc
     5581 ggcgcccgaa tggatgccgc cggggtaggt ctcatagacc aggcgctccc ccttttccgg
     5641 caccaggtcg ataaaaacat cccattccgt gcccatgtag ccgttccaca tgaagagctg
     5701 gccgaagacg atgcgctgat ccgcggtcgc cgaggcagtg gcgaggaagg aggagcattt
     5761 gtgcagccgc tccggcaggt ccatatcgat ctccggggcc ttgaagcgct gtccgctgag
     5821 ctgatgggga gtgttctgga ccgcgtccca ggcataggag aggtcgatgt aggagttgat
     5881 ggtgacgata tcgagcagtt cgaccgggcg gtcgaagagg gtcgcaccct cttttttggc
     5941 gccgtcggcg atgccctgca tctcctccat gaattcggtt tcatatttgc gcaggaagag
     6001 gagatcgacc tggctgcgca ggtcgcgcca gcccgcggcg ggattgtcgc ggttgcggct
     6061 ggtggcgagt ttggtgatga aggttttaag ctcccgggcg agcagggcgc catgctggaa
     6121 gccgcgctcg taggcagagc cttcgatgtg caggtaggtc cacccctgct cttcgaaacg
     6181 gaaggccttg ccgtaccagc ggatgcgttc ggcggggatg tactcggtga tgtcgtcgac
     6241 gggtgtgatg gcgatgtcgt cgaaccaggc ctcgccggcg gcgtcgccgc ggtagccgag
     6301 gcgcagctcg acgcggtctt cggcgcggga ggcgaaaaag agggcctcac tgagctgcca
     6361 ctcctgatcg ccgccgatcg ggcgggacca ggccgtgatg ggcatgccgc ccatacgcag
     6421 gcaggcgggc aggggagtgg gataatgctg tgccgagtcg gtgcgggccc cagaagttct
     6481 gatccagccg ctgaggcggt agagcttgcc gacctcgagc cgcaccggct gcgagagcag
     6541 ggtggagcgg cggggcgagg cgtggaggat gtgcaggctg ctctgaccgc tgcggatgac
     6601 ggcgcgatcg agactgatgc aggcggtgga gagggagtcc ggctgccagc cggcagcgcc
     6661 ctcagcctgc agggtttcaa aaccaggatt ggccagcggg atggactgcc cgggagctgc
     6721 ggccaggatc gaaacggccg ccagcagtac aggtgtgata agacgcatca ggcgtgaggc
     6781 tgcgaagggc atggaacctc cgaacaggac agggacgatg ggaagatttg caaaatgtac
     6841 gggatttcgc gcatgatttc aagaaaattt atcgcatccg gagattaatc ttgaatttgg
     6901 cggtagtgat gattaccttt acggcaatct ttctcctgcc gcgggggcga ccgcaggtgt
     6961 cgtatccgga cgcgatcgca gccgatgagc ggcgcgttct ccggcagccg gaagcgacct
     7021 gccggccaga tcagcatcga gaggctagcg tattttgtac cataaatcca aggagtcaga
     7081 tatggccagc agcagcatcg ccaaaccggt agaaccgcaa tccggaggag aggaaaaacg
     7141 gtacggcggc cccttcgcca tcatgacggt gctctttttc atgtggggat tcatgacggt
     7201 ctggaatgac ctgctcattc cgcgtttcaa ggaggctttc accctcaact atttccaggc
     7261 gatgctggtg cagttcgcct ttttcggggc ctacaccatt ggcgccctga tttactatct
     7321 tttctcgacg gtctccggag atccgatcaa tcgcatcggt tacaagaacg gcgtgatcat
     7381 tgggctgatc acctccgctg tcggcagcgc cctcttttat cccgctgctg tgatggtttc
     7441 ctacaccttc tttctggtcg cccttttcat tgtcggactg gggtttgcca tgctccagat
     7501 cgctgccaat ccctacgtca ctattctcgg gcccgagcac accgcttcca gccggctcaa
     7561 tctcagccag gctttcaatt ctctgggcac caccattggc ccggtcatcg gcggctacct
     7621 gatcttcaag gtcttcaacc agccgggcgc ccacggggcc gatgccgtca agacccccta
     7681 tctgctcttc gccggggtct ttcttctgct cgctctcgtc ttccgcttca tccatttgcc
     7741 caacttcagc aatgccgagc aggtttcgca cggcgcgggc gctctcaaac atccccatac
     7801 cgtgctgggc atgatcgcca tctttatgta cgtcggggga gaggtcgctg tcggcagcgc
     7861 catcatcaac tttctcggca cgcccaggct gggcggtctg acgcacgaga cggcgagccg
     7921 ctacctctcc ttctactggg ggggactgat gatcggccgc ttcatgggag ccttcgccct
     7981 cagcgacatg gccaaatcca gaaagcgctt gctggtggcc gtcatcccgg tcatcgcctt
     8041 ttttgttatt cttgcgctgg cgggcccggc cgaagccctg cgctacggcc tcttcctgct
     8101 catcctgctc gcggctttct atctcggtga gaacagcgcc catcgcatgc tcgccctctt
     8161 cagcggggtg atcatcgtcc tgcttctgac cggcatgacc gccagcggag tgacggcgca
     8221 gtggaccatc ctcagcgtgg ggctcttctg ctcggtgatg tggtccaata tcttttccct
     8281 cgcaatcgag ggactgggcg ccctcaagag ccagggctcc tcgctgctgg tgatgatgat
     8341 cctcggcggg gcgatcctgc cgccgctgca gggctggatc gccgacatgg ccggcatcca
     8401 gttctccttc atcgtgccga tgatcgcttt tttctatgtc gctttttatg gattgtacgg
     8461 atatcgggcg ggccgcaacc tgcaggcctg attccattta ctgcctcaac cggcgaattc
     8521 ccggcgcagc cgctttttca gggcgcaggc ctcgcgcaaa agggccaaag gcattctccg
     8581 cagctgcaaa cggctgtcgg ggtctgcgct ccagctcacc gggaattcac agattttttc
     8641 tcccccatgc atcgccctca ggatgatctc cgcgtcaaac agaaatccat cggtcgaaca
     8701 ggaccggtaa agcgcgcggc cgctctcccc cgcatagatc ttcaatccgg cctgggtatc
     8761 ggtcaattct tgcggcagac cgagcagacg agtgaaaagg cggcggaaga gccaggccga
     8821 aatgcgccgg ctcggggatt gaggcttgag gatacggcta gtgggcagac ggcgggaggc
     8881 gtgggcgatg gtgcaggcac cggactggat catcgcgagg ccgcggctga tctcgtcgta
     8941 ggggatacag aggccgctgt cgatgaagag gatgcggcga ccgcgggagc gcaccatccc
     9001 ttcacggacg gcgtggccct tgccgcggtg gggcgtcagg cggaggggga gcagttcggc
     9061 ggctccggcg gtcggccggg ctttttcggc cgcctccagt gtgccgtcgt cgctgccgtc
     9121 gtcggcgacg ataatctcgc ctttcaaacc atggcgggcg cagaaatcgg ccgccgcgcg
     9181 gatgtcgcct gcaatcttcc cgtgttcgtt gtagaccgga atgatgatgg aaagatcgac
     9241 agcaggggcg tcatccgcgg cggcggccat gaagaatcag atttccttga caaagatgtt
     9301 cttgaagtgg atcggggcgc ggctgtcatg gttctggagg ccgatatagc cttcaggggt
     9361 ataggatttg atcttgccgc gcggttcggc ccgccagttg accacctcga ccccgttcag
     9421 ttcgacggtg atggtgccgc caatgaagga aatcttgtaa tgattccact caccggtctc
     9481 cttgaaggcg agggttttgg gagcctcggc gtcgtaagcc gctccggtct gatggatgcc
     9541 ggtgccggcg tcatcgatct ggatctcgaa ggatttatag atatagtcat cgctggtcac
     9601 catctcgggc acgcgcagaa agatgcccga attggtaaag atttcatcgc acttgtagtc
     9661 cagttcgagg ataaagtcgc ggtatttcct ggcactgtac cagagcagcc ccatgccgcc
     9721 ctgaccggtg agttcacctg ttttctcgtc gaggaggaaa tggccggggc cgtaatggtt
     9781 ccagccgtgt ttggagtaac ggccgtccca gcgggggagg agctgctggt aatcctggaa
     9841 ataggtgata ctgcggatat actccagccc cttggcgatg ttgggcgagg gattggagga
     9901 ttcgtcctcg cgttcgtgct cgatggagag atagccatca tagtcctgca gcgtcagctc
     9961 ggcgaggata tcatgaatct tggccacccc gctgccgaag gggacatcct tggcctggtg
    10021 ggcatcgttg aaaagatcga ggtccttgag atgggcgtgg acgatgcgtc cacgcagcag
    10081 ggccatcgcc ttgagcggct cccagccgga gcgcatccag tgggcggtgt ccatgccgga
    10141 gccgatgcgg ggatcgagcc ctttgacttt cgccaatact gtggcaggca gggcgaaccg
    10201 gctgggcaag ggatggttgt ggatggcgac cttgatgttg tactccttga ccatcctctc
    10261 caggggacgc cagtcggtga aagcgggctc ggtgttgatc acctggatgc ccatcttgcg
    10321 ggcgaaatca aagacctccc gcatcgcttt ttctgtgctg tcaaagccga caacgccata
    10381 ggccaccagg gtcaggctgt tcttcgccag ctcatccttg acccaggcga tctgctcgtc
    10441 gctcatggtg tgcgaaaaga ggacgttggc gggcatctcc ttgctcagcc gttgacccgg
    10501 gtaggcctcg acgtagcgga tgcccagttc gcgcgttttg gcgagggctt cggcgaaggt
    10561 gtacttgcga aaggtgtagg tctgcatcgc cattttcatc tttttgatgc ggacatcctt
    10621 gtacggcccc gcggcctcct ggccccgaac cggcatcacc gccaggccga ggaaaaggac
    10681 gaggcaaatc ttgagcaaat ggtctgtcat atcagttctc tccggatgtt agtttttttg
    10741 ggtgagccag ccgcttattt gcggggattc caggcgccgc gcgcgaccgc cgggatgaaa
    10801 tcctcgatgc ccggcgccgg cagcagaata gtcttctcct gttccgcgct catgtaggcc
    10861 gacatcaaca gggtggtcac ctcgaggccg tcctcaaagg tctcgctcgg ccgcactccg
    10921 gccaggaaac agtcaaccat atggcggtcc tcgacgtcgt agccatactc attgccctcg
    10981 ttggagacca ccggcatcag acccatctcc gcattctgtt tctcgaccag atcctcgccg
    11041 gacctgcccg ccacctcgcg gctgaaaaag accttgagcc cggtgtcgag ggtgttgacc
    11101 tgcatcgaat attcggggcc gagcagctcc atcgaaaggc gcaggccggc gccgacgtag
    11161 caccaggagg tggtggtctc gaccacgacc tggttgccct cccggtcatg atattcgatc
    11221 agggaacggg cgaaatcctc ggagggccgg ttggtataat caagcttgcc cttgctgtac
    11281 tccgccaggt gtttggcata gtcggggcgc tgccacttga ggcagttggt ataagcggac
    11341 atcttgacgg gggtgagggc ctcccgcggc gcgccaggag gggtcagcag atagcgggcg
    11401 gtctccaccg agtggcacat catgtcgttg agcaccccgc cgccctgcag ctcgccctcc
    11461 cagaaccagg gcatgtgcgg tccggagtgc tcctccgagg cgcgcgccag ataggggcgg
    11521 ccggtcgcag cggcgccgcg cagccagacg atgtcgcggc cgcggatgac ggtgggggag
    11581 aagacctgat tttcgagata accgtccagg agccccgcct tttgggccag ctcgaccatc
    11641 cggcgcgcct cgcgcacgtt gcggccgagg ggtttttcac aggcgacccc gatcagctgg
    11701 cccttgccgc ttatcaccgc ctcggcgatc tcttccatga tctccacgcg ggtgaaattg
    11761 ggggcgcaga tccagattgc gtcgatggcg ggatcggcca ccatctcggt gacgctcttg
    11821 tagattttcc catcacccag attgagctgc ctgaccagag ccgccgcctg ctcggcttcg
    11881 gccgggctct tggcgacgat gccgttgata tcggcgttgc gcacactcac ccaggcgcgg
    11941 atatgaaact gcgccacaaa tccgcctccg acaaatccaa cccccagtac tttctttgcc
    12001 atgtgttgct ccctctttta tggttcaggc cggcagcgcc ggccggttgg ttgaattatc
    12061 ataaacgcat cagccacagc ccgcaatccg ggctgcagca gctcccggat caggccttga
    12121 ctgttttggc cgcattcctg cgagtgtcga ccctgaaaaa ggccaggaag gcgatcgcgc
    12181 agaggatggt cagcgcgcac ggcaccagga agacattggt ccagttggtg accccctgcg
    12241 aagtggtgaa catcgacttg atccagccgg cgaagaggct gccgacatag ttaccgaggc
    12301 cgtaggtcac cagggtgatg aagctctggg cggagttgcg gatgtcgctg ggggcgatgg
    12361 tgtcgaaata gataaaagcc gcggcgaaga aaaagacgaa gcaaaagccg tgcagcgaaa
    12421 gcgaggcgat caccagccag gccggcgaac cgatcgcgaa gatgatgtag cgcagcggcc
    12481 aggccaggat gccgatggtc agcaccgtgc ggatgccgta tttgggcagg gcccaaggca
    12541 gcagaaaagc caaaaccagg atttcagcag cctgggcgat gaccatcacc attggtgtgt
    12601 tttccttggc cactccgatg atcgatgatt ccagaaatgg agcggtcaat acatagtaga
    12661 acatgagctc ggtggcgaca acaaaggcga tgatggtgaa aatgaggacg ttgcggtcct
    12721 tgagcatctt gagggcctca atgaaggccc agggcttggc cgcattcttc tgcggcgggg
    12781 tgtgcggcag ggtgaaggac tgcaagccca tgatcagcga aaagatgccg gcgagcagca
    12841 gggtgtcagc cccgggggcg ctccccagat agcgccagcc cgtcagcagc agacccgcga
    12901 cgatccagcc gatcgtgccc caaacccgca cctggccgaa ctccttctcc gagtccttca
    12961 tgttgataaa agcaatcgag ttggtcaagg ccatggtcgg ggcgtagagc agggagtaga
    13021 ggaagaagag ccacatcatc gcggtgtaat cggtcagacg cgagatgtag atcagcagcc
    13081 cgccgccgat cagctgcagc acggcgatca ccttctcact ggagaagtag cggtcggcga
    13141 actggccgcc gatgaacggg gcgatgatcg tggccagcgg cagcaggctg tagatgacgc
    13201 cgatctgcat accgctgaaa tgcagggtgt tctcaagata ggcggagagc accggtgacc
    13261 aggcccccca gatggcatac tgcagaaaca tcatgattcc caaacgcacc ttgacgctca
    13321 tttgaacctc catcggttga tcggatgttt gacgtttact ggttgaaacc ctgatgccac
    13381 ttcgcctcat accccctgcg gcgcagcttt tcgctccgga ccgggccaga gcagcttgga
    13441 gatctccctg accaccacca cgctgccggc tgctagaata caaacgcccc aatctttcag
    13501 gcccagcccc actgtgccga aggctttctg caggaagggg atgtagat
//
LOCUS       DAOPWU010000058        13432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4697_length_13432_cov_4.872692, whole genome shotgun sequence.
ACCESSION   DAOPWU010000058 DAOPWU010000000
VERSION     DAOPWU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13432
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4697_length_13432_cov_4.872692"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1748
                     /locus_tag="PKM23_04905"
     CDS             120..1748
                     /locus_tag="PKM23_04905"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNY90845.1"
                     /translation="MSAPETAFAALEPLDEIVTVNPSTGEELGRLPALPRAGIDAAIE
                     RSRQAQAEWAQIPLAERRRKIRVLMELLLEERAAIAELIALEQGKPVAEAMAAEIVPC
                     LGVLRELLRITPGVLKPQRRPHQMLLFAHKRSALLRVPFGVVAVISPWNFPFSVPLPE
                     IAAALLAGNSVLFKPAPHAILIAGKIAELCTRAGFPAGLMQLLYLHDREADYLTAHAG
                     IDKILFTGSTAVGRRVMCNAAQNVVPVVLELGGKDPAIVMADADIERAAKGIVWGALF
                     NAGQVCASIERVYVERAAAAPFIAACLREMEGVRVGDPLDPDTDMGPLSSEAHLHKVL
                     LHIQEAVEKGARLLHGGTRIDRAGFFIQPALLVDVDHTMLVMREETFGPVLPVMIFDS
                     LDEAVRLANDSAYALSAYAYTSSKAMADRLLCDLEAGTVVINDSTMSWGEPTAPWVGH
                     KQSGIGLTHAELGLLELTRPKYVSYDRGQRAPNLWWYPYGPDSLRLFTAAADLLYNRH
                     LSRKLAALTPVLLQKRFLRSTHWAALLRHLNRLF"
     gene            1852..3204
                     /locus_tag="PKM23_04910"
     CDS             1852..3204
                     /locus_tag="PKM23_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNY90846.1"
                     /translation="MMMSRWKQIKDRFAAWSYWLARDFWHIDQHRFSRWRILITHQLL
                     LGMVVVRTFLKNKLFDTAGALVYTSLLALVPFIAVFFSLMKAIGLQETISRVLVYAFH
                     PLGEEAVQLLVPQIMAFVNNTNINTLGYIGFVFLLLSLLLIVSTIEYSFNSIWKVKRQ
                     RKMHRRFIEYTSFIIIGPIIGLLIISWIANRYQLWIDSTPDSSFLSNMLRNFGPWFVT
                     LFIIWYLLNFIPNTRVRFKSALIGAIVGTTMWMVANWTFAQFLDTAFIQGPQSVMYAR
                     FAVLPLLLLWLFVGWSILLFSSQLAYAHQNMDKLIWDRTHPYLSPMFYESLALKVLLR
                     ITSQYFHHGRSSSEEELSDYFSIPESVITTVAGELVNLQLLHSQNGGETRFTPAKHPA
                     QIRVAEALIELRNYDTVSRRHTRVDHLVRNVMTHTDDSMKGTWKDMTLQDLLEMLPQD
                     "
     gene            3408..4799
                     /locus_tag="PKM23_04915"
     CDS             3408..4799
                     /locus_tag="PKM23_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNY90847.1"
                     /translation="MFHRSLAVVIAAALSGAGPALAQMVLSEIMFNPAGNERYDEFVE
                     LYNFSASDTIELEGWLLSDSSGFGRILAAPGGGSRLAPRSYGLILVPGYFSNSTLYAS
                     RIPAGALLLTIDKAQFGSYGLNNSRPERVSLHRPDSSLAAAHAYTVPNEEGFSEEKLR
                     LEGGDGADNWGESTVLHGTPGFANSVTPMPVDSTLALMVINEIMANPAAGEAEWIECY
                     NRGDETVRLTGWRISDSDTLRKRLLVREEVTIPPQGYLVLTGEESLLSALPAQGCTGV
                     VVSDWPALNSAGDGVVLYDVLGRMIDAVWYTEEWGGDRGISLERLSPLVASSVRSNWG
                     SCADALGHTAGRANSLLVGRLPAQALIAVSPNPFSPDGDGFEDHLAISLELPERTARI
                     NLRIFDSRGRQVRFLCNYEPSGSRKSLFWDGLDDEGLRCPIGIYILFLEAFAESSEAY
                     MRIKKSCVLAGRL"
     gene            complement(4843..6156)
                     /locus_tag="PKM23_04920"
     CDS             complement(4843..6156)
                     /locus_tag="PKM23_04920"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNY90848.1"
                     /translation="MAHPSVTVFWFRRDLRLEDNAGLFHALQDGAPVLPLFIFDREIL
                     DDLEERQDRRVAFIHQAQQNLDARLAEHSSGLLVRQGRPLEVWRQLVAEFPIAAVHTN
                     RDYEPSALARDREVAALLAEHGIPFHSHKDQVIFDGDEVVKEDGRPYTVYTPYMRKWL
                     ARLTPEDLAAHPSEKLLGRMAQGVEGGVPPLAEIGFTPMAVESAPMVDEAIIRNYDRT
                     RDLPAVAGTTRLGVHLRFGTVSIRRLAALGRALNQTWLQELIWREFFMSILAHFPHVV
                     DQPFKAQYAAVPWREDRGDFARWCAGETGYPMVDAGMRELNATGFMHNRVRMITASFL
                     CKHLLLPWRWGERYFAKKLLDYDLAANNGNWQWAAGCGCDAAPYFRVFNPALQAAKFD
                     PQEAYVKRWVPEVESPAYPEPMVPHAAARIRALETYGRALKGTRE"
     gene            6310..7671
                     /locus_tag="PKM23_04925"
     CDS             6310..7671
                     /locus_tag="PKM23_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNY90849.1"
                     /translation="METGHEAEKPAAALERRLGVWAAIGVVIGVTIGSGIFRNPAVIA
                     ARVPDPALMILVWVLGGAITLCGALSIAELAGALPQTGGLYAYLREGWGRPVAFLFGW
                     AQLTLIRASALGAIATVFGAYCLRSFGVDPALHPQLSNYLAAGCIIFAAVVNVVGIRL
                     GAAIVGLSTIAKYGALALLVLIAFAIGHAHGGSIAHFAPGSGGSVDMGLFGLALISVL
                     WAYDGFADLSFASGEIKDPQRNLPRALIGGTLAVIAIYLLANAAYLYVIPMERFASSP
                     LIAADTMLALVGQAGVAIVSVVVMISTFGSVNGSLVTNPRIFFAMADDRMLFKPIAWV
                     HPRFKTPYMAIILAAALGVIFVLTRTFEQLTDTFVLAMWPFYGLGVASIYRLRRTRPD
                     LPRPYKVVGYPVVPAIFLAGVLYLLGNALVTDTFWTSIVFAVILAGLPVYWLFFRSKN
                     GPA"
     gene            7658..9193
                     /locus_tag="PKM23_04930"
     CDS             7658..9193
                     /locus_tag="PKM23_04930"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNY90850.1"
                     /translation="MGLRKDLRVWAMGWLALLAIFPNDLSAQPATRKEKVKAAAGVVE
                     RIFSAHAAARHLPGLAWGVIYDGGLVISGGCGLADLGQQRPATPQSLFRAASMSKSFT
                     AMAILQLRDAGRLRLDDPAALYLPELKALRPVTADAPEITLRHLLTHGAGLPEDNPWG
                     DRQLARTDAELLALLPEISMANAPGVAYEYSNLGFAILGQIVQRVSGMDFTVYTRELI
                     FKPLGMGSSIWEYAGAPPERLVRGYARSGASFTELPMEHHGAYGAMGGLITSVEDFAR
                     YAAFHLSAWPPRDEADAVPLRRSSLREMHHPWRFYSLLPDADPAAGAPCPAAQAYAYG
                     LRWQSDCGGMVAVGHSGGLPGFGSNWTMLPDYGIAVVSFTNLTYATTTAINRTVLDTL
                     IALAGLEPRQPPVSAILEKRKHELVGMLPEWQGARESGIFAENFFLDNPPAELARLSR
                     QLFAEAGEIRGAGPMRPLNNLRGSFLLEGSRKDISLFFTLTPERVPRIQELKMTLMEK
                     SGG"
     gene            complement(9204..10085)
                     /locus_tag="PKM23_04935"
     CDS             complement(9204..10085)
                     /locus_tag="PKM23_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNY90851.1"
                     /translation="MSKSELFEERELMNTGHVGCPGCGATIAMKLALRALGDKTMVVI
                     PACCWAIIAGNYPQSALKVPVLNTAFATTGAVCSGLRAALDLRGEKDTTIIGWAGDGG
                     TFDIGFQALSAAAERGDDFIYICYDNEAYMNTGVQRSSSTPWGASTMTTPGASWKRTQ
                     KKNIVEIMAAHRIPYTATANIAFPEDLERKLRKARSIRSGLRFIHLFASCPTGWGFAP
                     ADAIKISRMAVLSRAFPLYEVEEGERYTINSGGDAPVYDYLRMQNRFRRLSDGDIAMI
                     QEHVNREWERLNRKARG"
     gene            complement(10082..11245)
                     /locus_tag="PKM23_04940"
     CDS             complement(10082..11245)
                     /locus_tag="PKM23_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNY90852.1"
                     /translation="MKTILEGSQACAEAVRLARVQVVSAYPITPQTHIVEELAKFCSD
                     GSLNGRFLCVESEHSAMAAVIGAAAGGVRTFTASSSQGLALMHEMLHWAAGGRLPIVM
                     AEVNRALGPGWNLWADQTDSLAQRDTGWIQLYCADGQEVLDTTLQAYRLAEAVNLPVM
                     VILDAFFLSHTWEPVDLPAQEEVDQFLPPYAPRFRMDTDHPQSLHGVAPIGVYMEMRR
                     SMQLAMEAVPELLERIEEEFFARFGRRYGPVEPYLCDDADVILVTSGTAAGTARVVVD
                     QLRAEGQKAGLLRIRMFRPFPVRSVHEVLKSAQKVAVLDRNCSFGAGGIFASELRSAL
                     QGGNPVPVYSYIAGLGGRDITVETIRDCFALTRGTDRPAIESVWLGVKEELLK"
     gene            complement(11242..13026)
                     /locus_tag="PKM23_04945"
     CDS             complement(11242..13026)
                     /locus_tag="PKM23_04945"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNY90853.1"
                     /translation="MTPQDAKLPLLPLFMPISSAPTSANRTGSWSSRQPRYVEKTAPC
                     SVHCPCGEDIPRIEMLAVRGEYAQAWRTIMTENPLPGCCGRVCFHPCEGGCNRAQFDA
                     PVAINALERFLDDQAGAGPLPAELRPAPASGRRIAIAGSGPAGLAAAWFLARLGHECE
                     IFEAAPEPGGLLRYGIPAYRLPAAVLDREIGRIRELGVRIHCSMALDEELLRKTRSRY
                     DAIFIACGNGRGLGLDIPGGDLAIDGLAFLRAVRSGGLKTRPADESGPRSAAVIGGGN
                     TAIDVARTLLRLGIAPTILYRRRREEMPAFSHEIDSALAEGVRLVELSAPLAIRHDSQ
                     AFTLTLQKMRISEPGADGRMRVVPVEGETREMNFGAVYAAVGARAEEPWRSLAGAEGA
                     LRLSHTLLLPESPTGLPVLLGGDLVNEVESVADAIASGKEAAIALDLLFREGCGAITP
                     GLERCRIGAGPSLSMEIYQGGPRRSRSQRIVRYDDLNPDYFTAAPPERGDSLPPAAAA
                     GSFAEIEAALTADRALEQAGRCFNCGICNDCDNCRTFCPEAAVLLELRNSGRSDPERR
                     ITYDFCKGCGVCLTECPRCAMEMEEAAS"
     gene            complement(13023..>13432)
                     /locus_tag="PKM23_04950"
     CDS             complement(13023..>13432)
                     /locus_tag="PKM23_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNY90854.1"
                     /translation="VIVQDARLIHLVDVTKGLKSGGWILLNAPGLPADLAPFQGFKLA
                     CVDAAHIALEHGLGAKNQPITNTAMMGAFARLFSNPPLESVVEAIESEMPIKISENVA
                     AARAAYEAVVLHGQVNGSELLPSAGPIFRKAVS"
BASE COUNT         2217 a         4293 c         4366 g         2556 t
ORIGIN      
        1 caccccgatt tccctgatct cggtgggctc gaagcgcagc cagaccatct tcatggagtg
       61 attccgcctc cagccgaata tgccatgagc cacatcccgg agaagagaag gagctgactg
      121 tgagcgcacc cgagactgca ttcgctgccc tggagccgct ggacgagatc gtaaccgtca
      181 atccatccac tggtgaagaa ctggggcggc tgcccgccct gccgcgcgcc gggatcgacg
      241 ctgcgataga acgcagccgg caggcccagg ccgaatgggc gcagattccg cttgccgagc
      301 gccggcgcaa gatcagggtt ttgatggagc tgctcctgga ggagcgcgcg gccatcgccg
      361 aactcatcgc cctcgagcag ggcaagcccg tggctgaggc catggccgcg gagatcgtcc
      421 cctgcctcgg cgtgctccgc gagctgctgc gtatcacgcc cggtgtttta aagccgcaga
      481 gacgccccca tcaaatgctg ctctttgccc acaagcgcag tgccctgctg cgggttccct
      541 tcggggtggt cgcggtcatc tccccatgga atttcccctt ttcggtcccg ctgcccgaaa
      601 tcgccgcggc cctgctcgcc ggcaacagcg tgctcttcaa acctgcgccc catgccatcc
      661 tcatcgccgg taaaatcgcg gagctctgca cacgggcggg tttcccggcc ggcctgatgc
      721 aactgcttta cctccacgac cgcgaggccg attatcttac cgcccatgcc ggcatcgaca
      781 agatcctctt caccggcagc accgccgttg gacgcagggt gatgtgcaat gccgcacaga
      841 atgtcgtgcc ggtcgtgctc gagctggggg gcaaggaccc cgcaatcgtt atggcggatg
      901 cggatatcga acgcgccgcg aaggggattg tctggggagc gctcttcaac gccggacagg
      961 tttgcgcctc gatcgagcgc gtctatgtcg agagggcggc cgcggcgccc ttcatcgccg
     1021 cctgcctgcg tgagatggag ggggtgcggg tcggtgatcc cctcgatccc gataccgaca
     1081 tggggccgct cagctccgag gcccatctgc acaaggtcct gttgcatatt caggaggcgg
     1141 tggagaaggg ggcccggctc ctccacggcg gcacaaggat cgaccgtgcg ggttttttca
     1201 tccagcccgc actcctggtc gacgtcgatc ataccatgct ggtgatgcgc gaggagacct
     1261 tcggaccggt gctgccggtg atgatcttcg attcccttga tgaggcggtg cggctggcca
     1321 atgactcagc gtacgctctc tctgcctatg cctacacttc cagcaaggcg atggccgatc
     1381 gcctgctgtg cgatctcgag gccggcaccg tggtcatcaa tgacagcacc atgagctggg
     1441 gcgaacccac cgccccctgg gtggggcaca agcagagcgg gatcggcctg acccatgccg
     1501 agctcgggct gctcgaactg acccggccta aatacgtcag ctacgaccgg ggacagcgcg
     1561 cccccaatct ctggtggtat ccatatggtc cggattctct ccggcttttc accgccgccg
     1621 ctgacctcct ctacaaccgg catctgagcc gcaagcttgc cgccctgacg ccggtccttc
     1681 tgcaaaaacg ttttctgcgc tcaacccact gggctgccct cttgcgccat ctcaaccgcc
     1741 ttttctagcg gcccggagcg gcggcggttt tttcccctcc tccggccagc ttttgcttga
     1801 ctttcattcc ttttgtccgt atactttttc tgggagagac tataccaagg gatgatgatg
     1861 agccgctgga agcagataaa agaccgattc gccgcctgga gttactggtt ggcgcgcgac
     1921 ttctggcata tcgaccagca caggttctcc aggtggcgta tcctgatcac gcatcagctt
     1981 ctgctgggca tggttgtcgt tcgcaccttc ctcaagaaca agctcttcga caccgccggc
     2041 gcactggtct ataccagcct gctcgccctg gttcccttca tagccgtatt tttttcactg
     2101 atgaaggcga tcgggttgca ggagaccatc tcccgcgtgc tggtctacgc ctttcatccc
     2161 ctcggcgagg aggcggtcca gctgctcgtc ccgcaaatca tggccttcgt caacaacacc
     2221 aacatcaaca ccctcggcta catcggcttc gtctttctcc tgctttcgct gctgctgatc
     2281 gtctccacca tcgaatactc gttcaactcg atctggaagg tgaagaggca gcgcaagatg
     2341 caccgccgtt ttatcgagta taccagtttc atcatcattg ggccgatcat cgggctgctg
     2401 atcatcagct ggatcgccaa ccgctatcag ctttggatcg attctactcc agacagctct
     2461 tttttaagta acatgctgcg caatttcggc ccctggtttg tcaccctttt tatcatctgg
     2521 tatctgctca acttcatccc caacaccagg gtgcgtttca aatcggccct catcggtgcg
     2581 atcgtgggca cgacgatgtg gatggtagcc aactggacct tcgcccagtt cctcgacact
     2641 gcctttatcc aggggccgca gtcggtgatg tacgcccgct tcgccgtcct tcccctgctg
     2701 ctgctctggc tctttgtcgg ctggtcgatc ctgctcttca gctcccagct cgcttacgcc
     2761 catcagaata tggacaagct gatctgggac cgcacccatc cctatctctc gccgatgttt
     2821 tatgaatccc tggccctgaa ggtgctgctg cgcatcacct cccagtactt ccatcatggg
     2881 cggagctcaa gcgaagagga gctttcggat tatttcagca tccccgagag cgtcatcacc
     2941 accgtcgccg gggagctggt caatcttcag ctgctccaca gccagaatgg cggcgagacc
     3001 cgcttcactc cggccaagca cccggcccag atcagggtgg cggaagccct gatcgaactg
     3061 cgcaattatg acaccgtctc ccgccgtcat acccgcgtcg atcacctcgt tcgcaacgtc
     3121 atgacccata cggacgattc gatgaaggga acgtggaagg atatgaccct ccaggacctg
     3181 ctcgagatgc ttccgcagga ctaatcctgc gcccatcccc ctgcgctgca gggcctgtcc
     3241 gacggaatgg aaccccgcga tacgccgccc cggaacccgc ctgatcgcct gactttacgc
     3301 tgtgtgcatc ctctccaatc ccgctttcgc cttgcggctg agcccgccgg cgcgggagat
     3361 cgatgggcgt cttcactatt gcataatgtc cagcagctgg agggattatg ttccaccgtt
     3421 ccctggcagt tgttattgct gcggccctga gcggggccgg gcccgccctc gcccagatgg
     3481 tgctctcgga gatcatgttc aatcccgcgg gcaatgaacg ctatgatgag tttgtcgagc
     3541 tctacaattt ctcggcctcg gatacgattg agctggaggg ttggctcctt tccgacagca
     3601 gcggcttcgg ccgcatcctc gccgcgccgg ggggaggctc gcgccttgcg ccgcgcagtt
     3661 atggcctcat cctcgtcccc ggctattttt ccaacagcac cctctacgcc agccgcatcc
     3721 cggctggggc gctgctcctg accatcgaca aggcgcagtt cggcagctat ggccttaaca
     3781 attcccgtcc ggagcgcgtc tcgctccacc gccccgattc gagcctcgcc gctgcccatg
     3841 cctatacggt gcccaatgag gagggctttt cggaggagaa gctgaggctg gaggggggag
     3901 acggcgcgga caactggggc gagagcacgg tgctgcacgg cacccccggt ttcgccaaca
     3961 gcgtcacccc gatgcccgtg gacagcaccc tggccctgat ggtgatcaac gagatcatgg
     4021 ccaatccggc cgcaggggag gcggagtgga tagaatgcta taaccggggg gatgaaacgg
     4081 tgcgactgac aggctggagg atcagcgact cggacaccct caggaagcgc ctgctggtca
     4141 gggaggaggt gaccatcccg ccgcagggct atcttgtcct gaccggcgaa gagagcctgc
     4201 tctccgcgct cccggcccag gggtgcaccg gggtggtggt ctccgactgg ccggccctga
     4261 acagcgccgg cgacggggtg gtgctctatg acgtcctggg gaggatgata gatgcggtct
     4321 ggtatacgga ggaatggggg ggagaccggg gcatctccct ggaacggctg agccccctgg
     4381 tcgcctcttc ggtgcgcagc aactggggct catgcgcgga cgccctcggg catactgccg
     4441 ggagggccaa cagcctgctc gtcggccggc tgccggcgca ggcactcatc gcggtctcgc
     4501 ccaatccctt ctccccggac ggggatggtt tcgaggacca cctggccatc agcctcgagc
     4561 tgccggagcg caccgcccgg atcaatctgc gcattttcga cagccgcggc cggcaggtgc
     4621 gttttctctg caattacgag ccgagcggca gccgcaagag cctgttctgg gacggcctgg
     4681 acgatgaggg gctgcgctgc cccatcggca tctacatcct cttcctcgag gcctttgccg
     4741 aatcgagcga ggcctatatg cggatcaaaa agagctgtgt tctggcgggg cggctgtaga
     4801 tcgccggcaa tctcgcgccc acccaaaccc atgcaaaacc tgctactccc gcgttccctt
     4861 caaagcccgg ccgtaggttt cgagggcgcg gatgcgcgcc gccgcatgcg gcaccatggg
     4921 ttcaggatag gccgggctct ccacctccgg cacccagcgt ttgacatagg cttcttgagg
     4981 gtcgaatttc gccgcctgga gcgcggggtt gaagacgcgg aagtaaggtg cggcgtcgca
     5041 gccgcagccg gccgcccact gccagttgcc gttgttggcg gcgaggtcat agtcgagcag
     5101 cttcttcgca aaatagcgct cgccccagcg ccagggcagg aggaggtgct tgcagagaaa
     5161 gctggccgtg atcatgcgca cgcggttgtg catgaatccg gtggcattga gttcgcgcat
     5221 gccggcgtcg accatgggat agccggtctc gccggcacac cagcgggcga aatcgccgcg
     5281 atcctcacgc cagggcacgg cggcgtactg ggccttgaag ggctggtcga cgacgtgggg
     5341 aaaatgggcg aggatcgaca tgaagaattc gcgccagatc agctcctgca gccaggtctg
     5401 gttgagagcg cgaccgaggg cggccagccg gcggatcgag accgtgccga agcggagatg
     5461 gactcccaga cgggtggtgc cggccacggc cgggaggtcg cgggtgcggt cgtagttgcg
     5521 gatgatcgct tcgtcgacca tgggagcgct ctccaccgcc atgggtgtga agccgatctc
     5581 tgccaggggc gggacgccgc cctccacccc ctgcgccatg cgtccgagca gcttttcgct
     5641 cgggtgggcc gcgagatcct ccggcgtcag ccgcgccagc cattttctca tgtacggcgt
     5701 gtagacggtg tagggccggc cgtcctcctt gaccacctca tccccgtcaa agatcacctg
     5761 gtccttgtgg ctgtggaagg ggatcccgtg ttcggcgagc agggccgcta cttcacggtc
     5821 gcgcgcaagg gcggagggtt catagtcgcg gttggtgtga acggcggcga tggggaactc
     5881 ggcgaccagc tggcgccaga cctccagcgg ccggccctgg cgcaccagca ggccgctcga
     5941 atgctcagcc aggcgcgcgt cgaggttctg ctgcgcctga tggataaagg cgacgcggcg
     6001 gtcctgcctc tcctccagat catcgaggat ctcccggtcg aagatgaaaa ggggcagcac
     6061 cggggcgcca tcctggaggg catgaaaaag tccggcgttg tcctcgaggc ggagatcgcg
     6121 gcggaaccag aaaacggtga cggagggatg agccataaag gtccttttat gatggaacag
     6181 tcagccccgt aatatagcac tccccttttt gcaaatcaac tcgcaagtta aaaagcggtt
     6241 gaatttcatc cggcaatcca gtacctttta tccggagggg gagtgcacat tcaccaggga
     6301 gaagagaatt tggaaacggg acacgaagct gaaaaaccgg ctgccgctct ggagcgccgt
     6361 ctgggggttt gggcggccat tggcgtagtc atcggcgtca ctatcggctc gggcattttt
     6421 cgcaatccgg cggtgatcgc ggcgcgggtg cccgatcccg ccctgatgat tctggtctgg
     6481 gtgctcggcg gggccattac cctttgcggt gcgctttcga ttgcggagct ggcgggggcg
     6541 ctcccgcaga ccggggggct ttatgcctat ctgcgcgagg gctggggccg tccagtcgcc
     6601 tttcttttcg gctgggcgca gctcaccctg atccgcgcct ccgccctcgg cgcgatcgca
     6661 acggttttcg gcgcctactg tctgcgctcc ttcggcgtcg atcccgcgct ccatccccag
     6721 ctctccaatt atcttgcggc gggctgcatc atctttgctg ctgtcgtcaa tgtggtgggc
     6781 atccggctcg gggcggccat cgtcggcctc tcgaccatcg ccaagtacgg ggccctcgcc
     6841 ctgctggtgc tgatcgcctt tgcgattggc catgcccacg gcggctccat cgcccatttc
     6901 gccccaggtt ccggcggcag cgtcgacatg gggctcttcg gcctcgccct gatctcggtg
     6961 ctgtgggctt atgacggttt tgcggatctc tccttcgcca gcggtgagat caaggatccc
     7021 cagcgcaacc tgccccgcgc cctcatcgga gggaccctgg cggtgatcgc catctatctg
     7081 ctcgccaacg ccgcctatct ctacgtcatt cccatggagc gttttgccag ttcaccgctg
     7141 atcgccgccg acaccatgct ggccctggtc ggccaggccg gagtggccat cgtctcggtc
     7201 gtggtgatga tctccacctt cgggtcggtc aacggcagcc tggtgaccaa tccgcggatt
     7261 tttttcgcca tggccgacga ccggatgctc ttcaagccga tcgcctgggt ccatccgcgc
     7321 ttcaagaccc cgtacatggc gatcattctc gccgcggccc tgggggtgat tttcgtcctg
     7381 acccgcacct tcgagcagct caccgacacc tttgtgctgg cgatgtggcc cttttacggc
     7441 ctcggggtgg cctccattta ccgcctgcgc cgcacacgtc ccgatctgcc gaggccctac
     7501 aaggtggtgg gctatccggt ggtgccggcc atctttctgg ccggggtcct ctatctgctc
     7561 ggcaatgccc tggtgacgga taccttctgg acctcaatcg tcttcgccgt gatcctggcg
     7621 ggtttgccgg tctactggct gttcttcagg agtaaaaatg ggcctgcgta aggatttaag
     7681 agtatgggcg atggggtggc tggccctcct ggccattttc cccaatgatc tctctgccca
     7741 gcctgccact cgcaaagaaa aagtcaaagc ggccgcgggt gtggtcgagc ggatcttcag
     7801 cgctcacgcc gccgcgcgcc atctgccggg attggcctgg ggggtaattt acgacggcgg
     7861 gctggtgatc tcaggcggct gcggcctggc cgatctcggc cagcagcgcc cggccacacc
     7921 gcaaagcctt ttccgcgccg cctcgatgag caaaagcttc actgccatgg ccatcctgca
     7981 gctccgcgac gccggcagac tgcgcctgga tgatccggcc gccctctatc tgcccgaatt
     8041 gaaagccctg cgccccgtga ccgccgacgc ccccgagatc acccttcgcc acctgctcac
     8101 ccacggggcc ggcctgcccg aggacaatcc ctggggcgac cggcagctgg cgcgcacaga
     8161 tgctgagctg ctggccctgc ttccggagat ctcaatggcc aacgccccgg gcgtcgccta
     8221 tgagtacagc aacctcggct ttgccatcct cggccagatc gttcagcggg tctccggcat
     8281 ggatttcacc gtctataccc gcgaattgat cttcaagccc ctcgggatgg gctcctccat
     8341 ttgggagtac gccggggccc cgcccgagcg gctcgtccgc ggctatgccc gcagcggcgc
     8401 ttccttcaca gaactgccca tggaacatca cggggcctat ggcgcgatgg gcgggctgat
     8461 cacctcggtg gaggattttg cccgctatgc cgctttccat ctctccgcct ggccgccgcg
     8521 ggatgaggcc gatgcggttc cgctccggcg cagctccctg cgcgagatgc accatccctg
     8581 gcggttttac agcctcttgc cggatgcgga tcccgccgct ggcgcaccct gtcccgccgc
     8641 acaggcctac gcctatgggc tgcgctggca gtccgactgc ggcggcatgg ttgcggtcgg
     8701 ccacagcggg ggactgcccg gcttcggcag caactggacg atgctgcctg attacggcat
     8761 tgctgtggtc tctttcacca acctcaccta tgcgaccacc accgccatca accggacggt
     8821 gctggatact ctcatcgccc tggccggctt ggagccgcgg cagcccccgg tatcggcgat
     8881 cctggaaaag cgcaagcatg aactcgtcgg aatgctgccg gagtggcagg gggcacggga
     8941 gagcgggata ttcgcggaga atttctttct ggataatccg ccggcggagc tggccaggtt
     9001 gagccggcag ctctttgcgg aggccgggga gatccgcggg gccgggccga tgcggccgct
     9061 caacaatctg cgtggcagct tcctcctcga ggggagccgg aaggatatca gcctcttttt
     9121 caccctcacc cccgagcggg tgccgaggat tcaggagctg aagatgaccc tgatggagaa
     9181 aagcggcgga tgagcgggat ttctcagccg cgggccttgc ggttgagccg ttcccactcc
     9241 cggttgacgt gctcctggat catggcgata tccccatcgc tgagccggcg gaaacggttc
     9301 tgcatcctga gataatcgta aacgggcgca tcgccgccgc tgttgatggt atagcgctcg
     9361 ccctcctcga cttcgtagag gggaaaggcc ctgctcagga ccgccatgcg ggagatcttg
     9421 atggcatccg ccggggcgaa tccccagccg gtcgggcagg aagcgaagag gtggataaag
     9481 cgcaggccgc tgcggatgga tcgggccttg cggagtttgc gttcgaggtc ctcgggaaag
     9541 gcgatgttgg cggttgcggt gtaggggatg cggtgggcag ccatgatttc gacgatgttc
     9601 ttcttctgcg tccttttcca gctggcgccc ggggtggtca tggtgctggc cccccagggg
     9661 gttgaggaac tgcgctggac gccggtgttc atataggcct cgttgtcgta gcagatatag
     9721 ataaagtcat ccccgcgttc agccgcggcg ctgagggcct ggaagccgat atcgaaggtg
     9781 ccgccgtcgc cggcccagcc gatgatcgtg gtatcctttt cgccgcgcag atcgagggcg
     9841 gcgcgcaggc cgctgcagac cgctccggtg gtggcgaagg cggtattaag tacgggcacc
     9901 ttcaatgccg actggggata attgcccgcg atgatcgccc agcagcaggc agggatgacc
     9961 accatggtct tgtcgccgag ggcgcggagg gccagtttca tggcgatagt cgcgccgcag
    10021 ccggggcagc ccacatgccc cgtgttcatc agctccctct cctcaaacag ctcactcttg
    10081 ctcatttcag cagctcctcc ttcacaccca gccagacgct ctcgatggcg ggccggtcgg
    10141 ttccgcgggt caaggcaaaa caatcccgga tggtctccac ggtgatatcg cggccgccga
    10201 ggccggcgat gtagctgtag acgggaaccg ggttcccgcc ctggagcgcg ctcctgagtt
    10261 cgctggcgaa gatgccgccc gccccgaagg agcagttgcg gtcgagcacc gccacctttt
    10321 gcgccgattt taaaacttca tgcacgctgc ggacggggaa ggggcggaac atcctgatcc
    10381 gcagcagccc cgccttttgg ccctccgcac gcagctgatc cacgaccacg cgggcggtgc
    10441 cggctgctgt gccgctggtg accagaatga cgtcagcgtc gtcgcaaagg tagggttcga
    10501 ccggcccgta acgccgtccg aagcgggcga agaactcctc ctcgatgcgc tccaggagct
    10561 ccggaacggc ctccatggcc agctgcatgc tgcgccgcat ctccatatag accccgatcg
    10621 gggcgacgcc gtggaggctc tggggatggt cggtgtccat gcggaatctg ggggcatagg
    10681 gggggaggaa ctggtcgacc tcctcctgag cggggaggtc gacgggctcc caggtgtggg
    10741 agaggaaaaa ggcgtcgagg atgaccatta ccggcaggtt gacggcctcg gccaggcggt
    10801 aggcctgcag ggtggtatca aggacctcct ggccgtcggc gcagtagagc tgaatccagc
    10861 cggtgtcgcg ctgggcgagg ctgtcggtct ggtcggccca gaggttccag ccggggccga
    10921 gggcgcggtt gacctcggcc atgacgatgg gcaggcggcc gcccgcggcc cagtgcagca
    10981 tttcgtgcat cagggcgagt ccctgggagg agctggcggt gaaggtgcgg accccgccgg
    11041 cggccgcacc gatgactgcg gccatggcgg agtgttcact ctccacacag agaaagcgcc
    11101 cgttcaggga gccgtcgctg cagaatttgg cgagctcttc gacgatatgg gtttgcgggg
    11161 tgatcgggta ggccgagacc acctggacac gggccaggcg cacggcttcg gcgcaggcct
    11221 ggctgccttc gaggatggtt ttcatgaggc cgcctcctcc atctccatgg cacagcgcgg
    11281 acattcagtg aggcagaccc cgcagccctt gcaaaagtcg taggtgatcc ggcgctccgg
    11341 atcggagcgg ccgctgttcc gcagctccag caggactgcg gcctccggac aaaaggtgcg
    11401 gcagttgtcg cagtcgttgc agatgccgca gttgaagcag cgtccggcct gttccagcgc
    11461 ccggtcggcc gtgagggcgg cctcgatctc ggcgaaagag cccgcggcag cggcgggggg
    11521 cagactgtca ccccgttccg gcggggcagc ggtgaaatag tcaggattga ggtcgtcgta
    11581 gcgtacgatg cgttgggagc ggctgcggcg cggccctccc tggtagatct ccatcgagag
    11641 ggatggtccg gcgccgatgc ggcagcgttc gaggccgggg gtgatggcac cacatccctc
    11701 ccgaaagagg aggtcgaggg caatcgctgc ctccttgccc gaggcgatgg cgtcggcgac
    11761 gctctccacc tcattgacca gatccccgcc gaggagcacc ggcaggccgg ttgggctttc
    11821 cggaaggagc agagtgtggc tgagccggag ggcgccctcc gccccggcca gggagcgcca
    11881 gggctcctcg gcccgggctc cgacggctgc gtagaccgcg ccgaaattca tctcccgggt
    11941 ttcgccctcg accgggacga cccgcatgcg gccgtccgcc cccggctctg aaatgcgcat
    12001 cttttgcagg gtcagggtga aggcctggct gtcgtggcgg atggccagcg gggcgctcag
    12061 ttcgacgagg cgcacccctt cggccagggc gctgtcgatc tcgtggctga aggcgggcat
    12121 ctcctcgcgg cggcggcgat agaggatggt cggggcgatg ccgaggcgca gcagggtgcg
    12181 ggcgacatcg atggcggtgt tgccgccgcc gatgacggcc gcgctccgcg gccctgattc
    12241 atccgccggg cgggtcttca gcccgccgga gcggaccgcg cgaaggaagg cgaggccgtc
    12301 gatggccaaa tctccgccgg ggatgtcgag gccgaggccg cggccgttgc cgcaggcgat
    12361 gaaaatggcg tcgtagcgcg aacgggtttt tcggagcagc tcctcatcca gggccatgga
    12421 gcagtggatg cgcacgccga gttcccgaat cctgccgatt tcacgatcga ggacggcggc
    12481 gggcaggcgg taggcgggga tgccgtagcg cagcagtccg cccggctccg gggccgcttc
    12541 aaagatctcg cactcatggc ccagacgggc gagaaaccag gcggcggcga gtccggccgg
    12601 ccctgagccg gcgatggcga tgcgcctgcc gctggcgggc gcaggcctca attcagcggg
    12661 gagcggtccg gctccggcct ggtcgtcgag aaagcgctcg agggcgttga tggcaacggg
    12721 ggcgtcgaac tgtgcgcggt tgcatccccc ctcgcagggg tggaagcaga cgcggccgca
    12781 gcagcccggc agggggttct cggtcatgat cgtgcgccag gcctgggcat attcccctcg
    12841 aacagccagc atctcgatgc gcgggatatc ttcaccgcag gggcagtgga ccgagcaggg
    12901 ggcggttttt tccacatagc gcggctgccg gctgctccag gagccggtcc ggttggccga
    12961 ggtcggcgcg gaggagatgg gcatgaagag gggaaggagg ggaagctttg cgtcttgtgg
    13021 tgtcatgaga cggcctttcg gaagatcggg ccggcagaag gcaggagctc gctgccgttc
    13081 acctggccat gcagcaccac ggcctcatag gcggcgcggg cggcggcgac gttttcggag
    13141 attttgatcg gcatttcact ttcaatggcc tccaccacgg actcaagcgg ggggttggag
    13201 aagaggcggg cgaaggcccc catcatggcg gtgttggtga tgggctggtt tttcgccccc
    13261 aggccatgct ccagggcgat atgggcggca tccacgcaag ccagcttgaa accctgaaag
    13321 ggtgcgaggt cggccggaag cccgggagcg ttgagcagaa tccagccgcc ggacttgagg
    13381 cctttggtga catcgaccag atggatgagc ctggcgtcct ggacgatgac gt
//
LOCUS       DAOPWU010000059        13397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4714_length_13397_cov_4.768026, whole genome shotgun sequence.
ACCESSION   DAOPWU010000059 DAOPWU010000000
VERSION     DAOPWU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4714_length_13397_cov_4.768026"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKM23_04955"
     CDS             <1..447
                     /locus_tag="PKM23_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNY90855.1"
                     /translation="IVLFSIPMAVIGVTLTMVLTGTIFSLQAFIGCIMLAGIVVNNAI
                     LLVDHTNQLRRQEGMELMEAVATAGLHRLRPILMTTLTTVLGLLPLSFGLGEGGETQA
                     PMARVVIGGLTSSTLVTLVLVPVVYSLFEQKMISKKIRAVLGRIIK"
     gene            544..753
                     /locus_tag="PKM23_04960"
     CDS             544..753
                     /locus_tag="PKM23_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNY90856.1"
                     /translation="MRCNVGKTDRWIRIIVGLLLVGGNLFNYYVLHNPYQVWANIGWI
                     PLLTGLFRFCPLYLPFKISTVEKAR"
     gene            complement(815..2149)
                     /locus_tag="PKM23_04965"
     CDS             complement(815..2149)
                     /locus_tag="PKM23_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HNY90857.1"
                     /translation="MLLLSLLLPAVRLCSQPAHTRLIYSHSLLNATPGAGESIVNSGG
                     GFVAGKGWQATTENSQLRITLASALPFEGTFIINVTNFDPVSQNLPDDIKQHIINLYS
                     RIYDNNKDIFETDGSWCNIRTGVGYSTGEGVAGFKFLAAARGIDTRDEERCIEDHTWS
                     LTRTYEFKIIWTATYIYCYLDGSLMSRLPFAGQIEPFRYLLIGRDNLIWGYGAQPGVI
                     FSNVRIYGPQSSPVQLSGRLLLQGAYEVGSHAMRTPAAGAAVPLQSPFPQAPYAASSV
                     PAGIVDWVLVGLRSTTSGAPVVERSLWLRSDGRLIDPATAGTTVTLAAVTGSYYVTVQ
                     TRNHLAAMSATALVFDGLNESNCDFTTGTASYYGGKGAVQMEAGVWALQAGDINGDKA
                     VTAADFSLWQSAARTGTAGYAAADLNLDGCCTTRDYVLWFENARASAASGMP"
     gene            complement(2325..2948)
                     /gene="upp"
                     /locus_tag="PKM23_04970"
     CDS             complement(2325..2948)
                     /gene="upp"
                     /locus_tag="PKM23_04970"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNY90858.1"
                     /translation="MDNVHLVSHPLLDHSLTILRDRRTGTGEFRRHAAIVAQIVLLEA
                     TRSLRVRSRTVPTPLTSARGKQLAEELVLVPVLRAGLSMLEPALEILPQTPVGFIGLE
                     RDERTALARAYYQKFPIDLENKRIIVLDPMLATGGSLIDTLARLRDRGARDICAACIV
                     IAPEGVRALHKHYPEVAIHTAALDSHLNEAKYIVPGLGDFGDRYFGT"
     gene            complement(3065..3946)
                     /locus_tag="PKM23_04975"
     CDS             complement(3065..3946)
                     /locus_tag="PKM23_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90859.1"
                     /translation="MKQSRVIPWLVIFLLTGPGTIPGGELRKPTGREIDFHNQLVAAI
                     EKALPGGPLGWQLEERIGAAAPELVAADAAWRPFIVHYEVVWADPQRKKLAALDEKRS
                     IEKLEKAEEERALEEVEQKLRVLESEREDALQGRDMAAAQGLDREIENLRVQLDKKNS
                     WPGEEEEQMRKRLKNEGMRGTTLRISLDINLFALNWGELVPERITLPVQAGSYRCPGG
                     FSSEGEWQEGATMVLLGRNWRLSGDSPARWQADEPVGLPSTRTYSLVVRAQGDSSRTR
                     QYLESIDWMILDGLIFK"
     gene            4175..6271
                     /locus_tag="PKM23_04980"
     CDS             4175..6271
                     /locus_tag="PKM23_04980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNY90860.1"
                     /translation="MKEFTITLLCLAIGVGAVLAQDNPLLKEFKTPFGVPPFSKIKNE
                     HYLPAIQEGIRQHKLEIAAIASNPQPATFTNTIDALELSGQQLTRVTSVFFNMTSALT
                     SDVLQQIAQEAAPLLSSHSDDISLNTALFARIKAVYEQRAALGLSPEQEMVLTQYYRD
                     FIRGGANLDEAQKEQLRAINQELAVLTLKFGDNVLKETNEYALFVADAADLAGLPPAV
                     ISAAAEAAAERGQPGKWAFTLHNPSIFPFLQYAQNRDLRRQIYQAYIRRGDNNNALDN
                     KAILSKIAALRVRKANLLGYPSHANYILEENMAKKPEKVYALLDQLWRPALARARAEA
                     ADMQAIIDQEKGGFELAAWDWWYYAEKVKKAKYDLDEEALRPYFKLENVRSGVFGVAQ
                     KLYGITFTERKDIPVYHPDVRAFEVKEADGRHIGILMVDYFPRASKRGGAWMSDYRGQ
                     QKLGKVQTPVICNVGNFSKPTADRPALLSMDEVETMFHEFGHALHGLLSDVTYPKIAG
                     TSVARDFVELPSQIMEHWAFEPEVLRSYARHFQTGEVIPDELITKIEKSGHFNQGFAT
                     VEYLAASYLDMDWHTLREPIEHDANLFEEQALARIDLMPEIASRYRSPYFRHIFSGGY
                     SAGYYAYIWAEVLDADAFEAFRESSLFDQKSASAFRKYILSAGGSEEPMALYVKFRGR
                     EPGIEPLLKNRGLTGN"
     gene            complement(6284..11878)
                     /locus_tag="PKM23_04985"
     CDS             complement(6284..11878)
                     /locus_tag="PKM23_04985"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNY90861.1"
                     /translation="MKGWSLVFLMLALTGVTLAGDLQVQSVTPKGPVGAVTDALSIVV
                     TFSEPMVPLKATPEGLASGPLRISPAVKGKYRWMGTRTLVFTPLDTLPTATRFEMTIP
                     AGTKALSGAVLKAPYVWSFETLRPVLLYTAPESGEGGFDPRASFFLCFNLEMDPATVS
                     GRISLSDGARTVPLRFRHATFEELHRHWRLGDDSSRVIAATPAAPLARNKNYTLKLQP
                     GLTAKMGALGLAEERTITFVTIGDLVYRGFSSGSGDAPKVITPVSGIQFNFSNRVAPA
                     ELIKHLKFQPEVAIPDYYAERTWGVGELRLNLDFLPETRYRFTIDGELQDVYGSRIRS
                     AVTDSFRTAAYPARVSMNTGPGVLEAYGDRSYPVFFVNKEKVKLRLGLVAPERLIPLL
                     LSTDDLYGRAKPLPDSLFMIDRIWEIKQARNVKAAQPLKVDWLLGGRKTGVVLSELDD
                     LSTDPEIPVRYNRILLQVTDLGVSAKFSPLNNLVWVTNLKDAAPVAGARVEIRDDRNK
                     VLWSGVTGPQGLCETPGWRELGIASSNRWEKPRQWVFVYKDQDFAYNASDWGTGIFPY
                     RFGIEYEWNPEPVKYAGLLFTERGLYRAGETVHLKGMLREKRFHDWAPPGKKSYLFRL
                     SDSRGNEMVNQQITLSEFGSFDHDLKLPESAPLGYYYANLSDPPDSSGMEGESYMYTS
                     FRVEAYRPAEFSVRVRAAAPSFLLGDSAAAFVSANYLFGAPLAGQKVAWNAYLDRDVF
                     QPEGFEGFQFGVPEWYEEETERFSGRVLSQSRALLDEQGGARFAAPVLAAGVDFPLSL
                     TVSADVTSPGRQVIGASEKIIIHPASYYIGLKPSTSFAEAMKPLLFELTTVTPEGAAA
                     PGKSLKVRLVKRQWHSVRKAGSGGRYEWLSKAVDTPIDSFAVTTGAAAVTKSFTPREA
                     GVYFLEAVGREAGGRTTRTEAYLYVTGKGYVAWEREDDDRIELVADRSGYKPGQTARV
                     MVKSPFESARALVTLEREGVMSQQVLTLTGSTPTIEVPLTHKHLPNVFMSVILLRGRS
                     SDHLYSEEGEDLGRPAFKIGYVNLPVDPGNRHLKVAVKPDKSDYLPGESVTLDIDVRD
                     AAGEPSAAEVTLAVVDRGVLNLINYELPDPFDIFYGQRPLSVQSSETRLHVVEQRNYV
                     EKGEKRGGGGAEGFGSSADLRKNFKATAYWNPALDVDASGRAKVTFKLPDNLTTFKIM
                     VVANSIASEFGYGSSELKVNQPVMLLASLPRFARIGDLFEAGAVIHNYSGRSGEATLN
                     LQVSGLELQGPASATVTVPQGGSREVRFKFKAVQEGKAIFQFRCQMGGFSDGLERTIP
                     VQTPGVRESVALYQRADGPAQQSLEIPQEAWKELSTLELTLASTALSELSGPFEYLAY
                     YPYECLEQRLSRALPVLVSGDLVEAFGLQVKGVRDYRTIVQGVLADLGRFRTDEGGFA
                     LWQGSNYSSPYVTAYAAWAVTLAKRAGGYEVDETLFEEALAYMKNLIDGQFYGASPYD
                     ETSWQTTRALMLYVLALNGQADAGALDRLISAKAPMTVTGQAWLLKTVNHLKRKDGSA
                     AKLSQALLNKIRVTPTEVHFTSENNSPWIYDSDVRTTAVVLQALLETGAAFPQAEQAV
                     EWLMTERRQGRWSNTQENFFVLYALSEYFRKYETETPQFTARVRLAGREVMSKLFSGR
                     TAGVERRVISLTDYQEKKVQAEIAKEGPGTLYYGLRMNYYPREYDDAREEGITLVKVM
                     EPLTSGRSAGEKIPAGEVVKVTLTVIVPQARHYVVVDDPLPAGMEAVNATFATTSAAT
                     AMLGEGEESEEAWWYGFTHIEKRDDRVLLFADWLPEGAHSYSYLMRATTPGTFTLPPA
                     RAEEMYKPEVYGRTTGGELRIE"
     gene            complement(12057..13304)
                     /locus_tag="PKM23_04990"
     CDS             complement(12057..13304)
                     /locus_tag="PKM23_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNY90862.1"
                     /translation="MNIYGLVILGALLISYALNILARVLNLRTLGDPLPAEFEGVYDA
                     RDYARMQEYTRANTRFGFITATFDLAVLLLFWFAGGFNWLDLLVRGWGFSPLVSGLLY
                     IGLLLLGSSLLSLPFSIYDTFVIEERFGFNRTTVRTFVLDLLKGLALSVLLGGPLLAG
                     LLAFFGHAGEWAWFYAWLVTAGYSLLVSFVAPAWIMPLFNKFTPLAEGELKSAILAYA
                     EKVRFPLKGVFVMDGSKRSNKSNAFFTGFGRNKRIALFDTLIEKHATDELVAVLAHEI
                     GHYKKRHILTGMLISIAHMGIIFFLLSLFIRLAGLHEAFGMEQISLYAGLLFFGLLYS
                     PVEMLLGPLMNLLSRRHEFEADRFAAGTSGLGEALITALKKLSRDNLSHLTPHPFQVF
                     LHHSHPPVLKRIESIRMHHSGAV"
BASE COUNT         2584 a         4161 c         4167 g         2485 t
ORIGIN      
        1 atcgtcctct tttccatccc gatggcggtg atcggcgtga ctctcaccat ggtcctgacc
       61 ggcaccatct tcagcctgca ggccttcatc ggctgcatca tgctcgccgg catcgtcgtc
      121 aacaacgcca ttcttctggt cgatcatacc aaccagctgc gccggcagga gggcatggag
      181 ctgatggagg cggtcgccac agccggattg caccggctgc ggccgatcct catgaccact
      241 ctcaccaccg tgctcggcct gctccccctc tcctttggat tgggcgaggg cggcgagacc
      301 caggccccga tggcgagggt ggtgatcggc gggctgacca gctcgaccct ggtaacgctc
      361 gtgctggtgc cggtggtcta ttccctcttt gagcagaaga tgatctccaa aaagatccgg
      421 gccgtactgg gccggatcat caagtaatgc ggcacaagcg gaaatatagc gctctccctg
      481 ggtgttctat tcggcataga caagcatggc agtgataaaa catccaaaga gaggatagat
      541 gatatgagat gtaatgtcgg caagacggat cgctggatcc gcatcattgt gggtctgctg
      601 ctggtcggcg gcaatctttt caattattat gttctgcaca atccttatca ggtctgggcc
      661 aatatcggct ggatccccct cctgaccggg ctcttccgtt tctgcccgct ctatctgccc
      721 ttcaagatct cgacggtcga gaaggccagg taaatcaaaa agcccggatc aatcacagcc
      781 tgatccgggc tttttctctt ccagcctgca accatcacgg cattccgctc gccgcggatg
      841 cgcgggcgtt ctcgaaccag agcacatagt ccctggtcgt gcagcagccg tccagattga
      901 ggtcagccgc ggcatatccc gccgtgccgg tgcgggcggc gctctgccag agcgaaaaat
      961 ccgccgcagt gaccgccttg tccccgttga tatcccccgc ctgcagggcc caaacgcccg
     1021 cctccatctg caccgcccct tttcccccat aataactcgc cgttccggtc gtgaaatcgc
     1081 agttcgactc attcaggccg tcaaagacca gggcagtggc ggacatggcc gccagatggt
     1141 tgcgggtctg gacggtgaca tagtagctgc cggtgaccgc agccagggtc acggtggtgc
     1201 cggcagtggc cggatcgatg aggcggccgt cgctgcgcag ccagaggctg cgctcgacca
     1261 ccggtgcccc ggaggtggtg ctgcgcagtc ccaccagcac ccagtcgacg atgccggccg
     1321 gaacggagga ggctgcgtag ggcgcctgcg gaaagggaga ctgcagcggc accgccgcgc
     1381 cggcggccgg tgtgcgcatg gcatggctgc ccacctcgta ggcgccctgg agcaagagcc
     1441 ggccgctgag ctgcaccggt gagctttgcg ggccgtagat gcgcacatta gaaaagatca
     1501 ccccgggctg ggcgccatag ccccagatca gattgtcgcg gccgatgagc agatagcgga
     1561 agggctcgat ctgccccgca aagggcagcc gcgacatcaa gctcccgtcg agataacagt
     1621 aaatgtatgt agccgtccag atgatcttga actcataggt acgggtcagg ctccaggtgt
     1681 ggtcctcgat gcagcgctcc tcatcgcggg tgtcgatacc gcgcgcggcg gcgaggaact
     1741 tgaatcccgc cacgccctcg ccggtcgaat agcccacgcc cgtgcggata ttgcaccagg
     1801 agccgtcggt ctcgaaaata tccttgttgt tgtcgtagat gcgcgaatag agattgatga
     1861 tgtgctgttt gatatcatcg ggcaaattct gcgagaccgg gtcgaagttg gtaacgttga
     1921 tgatgaaggt gccttcaaag gggagggctg aggccagggt gatgcgcagc tgactgtttt
     1981 ccgtcgtagc ctgccagcct ttgccggcga caaagccccc gccgctgttg acaatgctct
     2041 cgcctgcgcc cggggtggcg ttgagcaggc tgtggctgta gatcagacgg gtatgagcag
     2101 gttgagagca gagccgcaca gcgggtaaaa gcagagaaag caggagcacc agaaaccggc
     2161 tccatgcggc cggtttccgt ccagtcgggg tattcctcac gatgccgtcc tcagttgcta
     2221 agagtctgat aatgcttttg atggcagaaa ttgcagaggc tgccgggcag gttcgtgcaa
     2281 gaagcatacc agggcgggag gagggattga catctgctgc tctttcaggt gccgaaatag
     2341 cggtcgccga aatcccccaa accaggtacg atatattttg cttcattgag atggctgtcg
     2401 agagcggcgg tatggatggc gacctctggg tagtgtttgt gcagggccct gaccccctcc
     2461 ggggcgatga cgatgcaggc ggcgcagata tcccgtgcgc cccggtcgcg caggcgggcg
     2521 agggtgtcga tgagggagcc cccggtggcg agcatgggat cgaggacgat gatgcgtttg
     2581 ttttcgagat cgatgggaaa tttctggtaa taagcgcgcg ccagggcggt tctctcatcg
     2641 cgctcgaggc cgataaagcc gaccggggtt tgcggcagga tctccagggc cggttcgagc
     2701 atggagagcc cggcgcgcag caccggcacc agaaccagct cttccgcaag ctgcttaccc
     2761 cgggctgagg tgagcggcgt gggcacagtg cggctgcgca cccgcaggct gcgggtggct
     2821 tcgagcagca cgatctgggc tacgatcgca gcgtggcgcc ggaactcccc ggtgccggta
     2881 cggcggtcgc gcaggatggt gagggaatgg tccagaaggg gatgcgagac gagatgcaca
     2941 ttatccatgg atgggggttc cttctttgga ttgattggcg ccgtacactg gtgggcggcc
     3001 agctttgccc agccggcaga ttgcctcctt ctgccggctc tcggctatat ggattgctcc
     3061 gtctttattt gaaaatgagt ccgtccagga tcatccagtc gatcgactcg aggtattggc
     3121 gcgtccggct gctgtcgccc tgggcccgga caaccaggct gtaggtgcgg gtggagggca
     3181 gccccaccgg ttcgtccgcc tgccaacgcg ccggggaatc gccgctcagc cgccagttgc
     3241 ggccgagcaa aaccatagtc gccccctcct gccactcccc ctcgctgctg aatcctcctg
     3301 ggcagcgata gctgccggct tgaaccggca gggtgattcg ttcgggaaca agctcccccc
     3361 agttgagcgc aaagagattg atgtcgaggg atatgcgcag cgtggtgccg cgcattccct
     3421 catttttcag tctctttctc atctgttctt cctcttcccc gggccagctg ttttttttgt
     3481 cgagctgcac cctgagattt tcgatctccc ggtcaagccc ctgcgccgca gccatatccc
     3541 ggccctgcag ggcatcctct ctttcgctct ccagcacgcg gagcttttgt tccacctcct
     3601 caagtgcgcg ctcctcttcg gccttctcca gcttctcgat cgatcgcttc tcatccaggg
     3661 ccgccagttt tttgcgctgg ggatcggccc agacgacctc gtaatggacg atgaaggggc
     3721 gccaggcggc atccgccgcg accagctccg gcgccgccgc gccgatgcgc tcctcaagct
     3781 gccaacccag tgggcctccg ggcagggcct tctcaatggc agccaccaac tggttatgga
     3841 agtcgatttc gcgtccggtc ggtttgcgca gctctccgcc gggaattgtg ccgggtccgg
     3901 tcagcaaaaa aatgaccagc caggggatga ctctgctttg cttcatgtgt aggcctttca
     3961 ctccgcctct cacatcctgc ctgccggcct gcaacgagtg cacgccgccg tggccaggcg
     4021 gcaggaggat tcacgtcagc actccttgtg cggcgcaagg gttcagatgg gacccggttt
     4081 caggggagca ttctgctgaa ccaaatacca ttgcgcattt gcggtttttt tgctattttg
     4141 aggcgaatcc aaatgatccc aacgcgagga gcccatgaaa gaattcacca tcaccctgct
     4201 ctgcctggcc atcggcgtcg gcgccgtact ggcccaggac aatcccctgc tcaaggagtt
     4261 caagaccccc ttcggtgtcc cgcctttcag caagatcaag aacgagcact acctcccggc
     4321 cattcaggag ggcatccgcc agcacaaact tgagattgcg gctatcgcct ccaatcccca
     4381 gccggccact tttaccaata ccatcgacgc cctcgagctc agcggccagc agctgacccg
     4441 cgtgaccagc gtctttttca acatgacctc cgccctcacc agcgatgtcc tgcagcagat
     4501 cgcccaggag gcggcgcctc tgctctccag ccacagcgac gacatcagcc tcaacaccgc
     4561 cctctttgcg cgcatcaagg cggtttatga gcagcgcgcc gcgctcggtc tgagccctga
     4621 gcaggagatg gtgctcaccc agtactatcg cgactttatc cgcggcgggg ccaatctcga
     4681 tgaggcccaa aaggagcagc tgcgcgccat caatcaggag ctggcggtgc tgaccctgaa
     4741 attcggcgac aatgtgctca aggagaccaa cgaatacgcc ctcttcgtcg ccgatgccgc
     4801 cgatctggcg ggcctacccc cggccgtcat ctccgccgcc gccgaggccg ctgccgaacg
     4861 cggccagccc ggcaaatggg ccttcaccct gcacaatccg agcattttcc cctttctgca
     4921 gtatgcgcag aaccgcgatc tgcgccgcca gatctatcag gcctacatcc gccgcggtga
     4981 taataacaat gccctcgaca acaaggccat tctgagcaag atcgccgccc tgcgcgtgcg
     5041 caaggccaac ctcctcggct acccgagcca cgcaaattac atccttgagg agaacatggc
     5101 gaaaaagccg gagaaggtct acgccctgct cgatcagctc tggcgccccg ccctggcgcg
     5161 ggcgagggct gaagcagccg acatgcaggc gatcatcgac caggaaaagg gcggctttga
     5221 gctcgccgcc tgggactggt ggtactatgc cgaaaaggtg aaaaaggcta aatatgacct
     5281 cgacgaggag gccctgcgcc cttacttcaa gctcgaaaac gtccgcagcg gggtctttgg
     5341 cgtggcgcaa aaactctacg gcatcacctt caccgaacgc aaggatatcc cggtctacca
     5401 tcccgacgtc cgtgcctttg aggtcaagga ggcggacggc cgccacatcg gcatcctcat
     5461 ggtcgactat ttcccgcgcg ccagcaagcg cggcggggcc tggatgagcg attatcgcgg
     5521 ccagcaaaag ctcggcaagg tgcaaacccc ggtgatctgc aatgtcggca atttctcaaa
     5581 accgaccgcc gaccggcccg ccctgctcag catggatgag gtcgagacca tgttccacga
     5641 gttcggccat gccctgcacg gcctgctcag cgacgtcacc tatcccaaga tcgccggcac
     5701 ctcggtggcg cgcgatttcg tcgaactgcc ctcgcagatt atggaacact gggccttcga
     5761 gccggaggtg ctcagaagtt atgcccgcca cttccagacc ggcgaagtca tacccgatga
     5821 gctgatcacc aaaatcgaga aatcgggcca tttcaaccag ggtttcgcca cggtcgagta
     5881 tctggccgcc tcctatctcg acatggactg gcacaccctg cgcgaaccca tcgagcacga
     5941 tgccaacctc tttgaggaac aagccctggc gcgcatcgac ctgatgcccg agatcgcctc
     6001 gcgctaccgc agcccctatt tccgccatat cttctccggc ggctactcgg cgggctatta
     6061 cgcctacatc tgggccgagg tgctcgatgc ggacgccttc gaggccttca gggagagctc
     6121 gctctttgac cagaagagcg ccagcgcctt ccgcaaatac atcctctcgg ccggcggcag
     6181 cgaggagccg atggccctct atgtcaaatt ccgcggtcgc gagcccggca tcgaaccgct
     6241 gctcaagaac cgcggattga ccggcaactg atgatgctcc ggatcattcg atccggagtt
     6301 caccgcctgt ggttcggcca tagacctcag gtttgtacat ctcctcggcc cgcgccggcg
     6361 gcagggtgaa ggtgcccggg gtcgtcgccc gcatcagata gctgtagctg tgcgccccct
     6421 cggggagcca gtcggcgaag agcagcacac ggtcgtcgcg cttttcgata tgggtgaacc
     6481 cgtaccacca ggcctcttcc gactcctccc cctcccccag catcgcggtc gcggcgctgg
     6541 tagtggcgaa ggtggcattg accgcttcca tcccggccgg caggggatcg tccaccacca
     6601 catagtggcg cgcctgcggc acaatcaccg tcagggtcac cttgaccacc tccccggccg
     6661 gaattttttc cccggcgctc cggccggagg tcaagggctc catcaccttg accagggtga
     6721 tcccctcctc gcgggcgtcg tcatactcgc ggggataata gttcatgcgc aagccgtagt
     6781 agagggtacc cggtccctcc ttggcgatct ccgcctgcac ctttttctcc tggtaatccg
     6841 tcagagaaat caccctgcgc tccaccccgg ctgtgcgacc ggagaagagc ttgctcatca
     6901 cctcccggcc agctaggcgc acccgtgcgg tgaactgcgg ggtctcggtc tcatatttcc
     6961 ggaaatactc ggagagggcg tagaggacga aaaagttctc ctgggtgttg gaccagcggc
     7021 cctggcggcg ttcggtcatc agccactcca ccgcctgctc ggcctgggga aaggccgccc
     7081 cggtctcgag cagggcctgc agcaccactg ctgtcgtgcg cacatccgaa tcgtaaatcc
     7141 agggggaatt attctctgac gtgaaatgga cctcggtggg ggtgaccctg atcttgttga
     7201 gcagagcctg cgagagcttt gccgcgctgc catccttgcg tttgaggtgg ttaacggtct
     7261 tgagcagcca ggcctggccg gtgacggtca tcggggcctt ggcgctgatg aggcgatcaa
     7321 gagccccggc atcagcctgg ccgttgaggg cgagaacgta gagcataagg gcgcgggtgg
     7381 tctgccagga ggtctcgtca tagggcgagg ctccgtagaa ctgaccgtca atgaggttct
     7441 tcatataggc cagggcctct tcgaagaggg tctcatcgac ctcatagccc ccagcccgtt
     7501 tggcgagggt cacggcccag gcggcgtaag cggtgacgta gggcgagctg tagttcgaac
     7561 cctgccagag ggcaaagccc ccctcgtcgg tgcggaatcg gcccagatcg gccagaaccc
     7621 cctgcacgat cgtccggtaa tcgcggacgc ccttgacctg cagaccgaag gcctcgacca
     7681 gatcgcccga gaccagcacc ggcagggcgc gagagaggcg ctgctccaga cactcatagg
     7741 gatagtaggc caggtactca aagggcccgg agagttcgga aagggccgtc gaggccaggg
     7801 taagctcgag ggtggagagc tctttccagg cctcctgcgg aatttcgagg gactgctgcg
     7861 ccgggccgtc ggcgcgctgg tagagggcca cgctctcgcg cactcccggg gtctggaccg
     7921 ggatcgtgcg ctcgaggcca tcgctgaatc cgcccatctg gcagcggaac tggaagatcg
     7981 ccttgccctc ctgcacagcc ttgaatttga agcgcacctc ccggctgcct ccctggggca
     8041 cggtgacggt cgcgctggcg ggtccctgca gctcgaggcc gctgacctgc aggttcaggg
     8101 tcgcctcgcc gctccggcct gaatagttat ggatgacagc gcccgcctcg aagagatcgc
     8161 cgatgcgggc gaaacggggc agcgaggcca gcagcatcac cggctggttg accttgagtt
     8221 cgctgctgcc atacccgaat tcggaggcga tcgaattagc caccaccatg atcttgaagg
     8281 tggtcagatt gtcaggcagt ttaaaagtca ctttggcgcg cccgctggca tcgacgtcga
     8341 gggccggatt ccagtaagcg gtggccttga agttcttgcg caggtcggcc gaactgccaa
     8401 atccctcagc cccgccgccg ccgcgtttct cgcccttctc cacgtaattg cgctgttcga
     8461 ccacatggag gcgggtctcg gaactctgca ccgagagcgg gcgctgccca tagaaaatgt
     8521 cgaagggatc gggcagttca taattgatca ggttgaggac gccgcggtcg accaccgcca
     8581 gggtgacctc cgcggctgag ggctcgcccg ccgcatcccg gacatcgatg tcaagggtca
     8641 cactctctcc gggcagataa tcgctcttgt ccggtttgac cgccaccttg aggtggcggt
     8701 tgccgggatc gaccggcaga ttgacatagc cgatcttgaa ggccgggcgg ccgagatcct
     8761 cgccctcctc ggaatagaga tgatcgctcg aacgcccgcg cagcaggatg accgacatga
     8821 agacattggg cagatgcttg tgcgtcaggg gcacctcgat ggtcggggtg ctgccggtga
     8881 gggtcagcac ctgctggctc atcaccccct cgcgctcgag ggtcaccagg gcccgggcgc
     8941 tttcgaaagg cgatttgacc atcaccctgg ccgtctgccc cggcttgtag ccggagcggt
     9001 cggcgaccag ctcgatgcgg tcatcatcct cgcgctccca ggcgacgtaa ccctttccgg
     9061 tcacataaag ataggcctcg gtccgggtcg tccgcccgcc ggcctcgcgg cccacggcct
     9121 cgaggaaata gacccccgcc tcccggggtg tgaagctttt cgtgacagcg gcggcgccgg
     9181 tcgtcaccgc gaaggaatcg atcggggtat cgaccgcctt gctcagccat tcatagcggc
     9241 cgccgctgcc ggccttgcgc accgagtgcc attggcgctt gaccaaccgc accttgaggc
     9301 tcttgcccgg cgcagccgct ccctcgggcg tcaccgtcgt cagctcaaaa agcagcggct
     9361 tcatcgcctc ggcaaacgag gtggagggct tgaggccgat gtaataggag gcgggatgga
     9421 tgatgatctt ttcactcgcc ccgatcacct gccggccggg actggtgaca tcggcgctga
     9481 ccgtcaggga gagaggaaaa tccactcctg cggccaggac cggggcggca aaacgggccc
     9541 ctccctgctc gtcgagaagt gcacgcgact gggagaggac gcggccggag aaacgctcgg
     9601 tctcctcctc gtaccattcc ggaaccccga actgaaaacc ctcaaatccc tcaggctgga
     9661 agacgtcgcg gtcgagatag gcgttccagg cgaccttctg cccggccaga ggcgcgccga
     9721 aaagataatt ggctgagaca aaagccgcgg cgctgtcgcc gagcaggaac gagggagctg
     9781 cagcgcgcac ccgcaccgag aattcggcgg ggcggtaggc ctccacccgg aaggaggtgt
     9841 acatgtagct ctcgccctcc atgccgctgc tgtcgggcgg atcgctcaga ttggcataat
     9901 aatagcccag aggcgcactc tccggcagct tgagatcatg gtcgaaagag ccgaactcgg
     9961 agagggtgat ctgctggttg accatttcgt tgccgcggct gtcgctgaga cggaacaaat
    10021 agctcttctt gccggggggg gcccagtcgt ggaagcgttt ctcgcgcagc atgcccttga
    10081 ggtggacggt ctcgccggcg cggtagaggc cccgctccgt gaagagcagt ccggcgtact
    10141 tgaccggctc gggattccat tcatattcaa tgccgaaacg gtaggggaaa atgccggttc
    10201 cccagtccga ggcgttgtag gcaaagtcct gatccttgta gacgaatacc cactgccgcg
    10261 gtttttccca gcgattggac gaagcaatac ccagctcgcg ccagcccggg gtttcacaca
    10321 gcccctgggg cccggtgacg ccgctccaga gcaccttgtt gcggtcatca cggatctcga
    10381 ccctggcgcc ggccacaggc gcggcatcct tgaggttggt cacccagacc agattgttga
    10441 ggggcgagaa tttggccgaa accccaaggt cggtcacctg caggaggatg cggttgtagc
    10501 gcaccgggat ctccggatcg gtcgaaaggt catccagctc cgaaagcacc acgccggtct
    10561 tgcgtccgcc gaggagccaa tccaccttga ggggctgcgc cgccttgaca ttgcgcgcct
    10621 gcttgatctc ccagatccgg tcgatcatga aaagcgagtc gggcaggggt ttggcgcggc
    10681 cgtagaggtc gtcagtggag agcagcaggg gaatgagccg ctccggcgcg accaggccga
    10741 ggcgcagctt gactttctcc ttgttgacga aaaaaaccgg atagctgcgg tctccgtagg
    10801 cctcgagcac ccccgggccg gtgttcatcg agacccgggc gggataggcc gccgtgcgga
    10861 atgagtcggt cacagcgctg cgaatgcggc tgccgtaaac gtcctgaagc tcgccgtcga
    10921 tggtgaagcg gtagcgggtc tcgggcagga aatccagatt gagacggagc tcaccgactc
    10981 cccaggtgcg ctcggcgtaa tagtcgggga tggccacctc cggctggaat ttgagatgct
    11041 tgatcagttc ggcaggggcg acccggttgc tgaaattgaa ctggatgccg ctcaccggag
    11101 tgatcacctt cggcgcatcg ccacttccgg aggagaagcc cctgtagacg agatcgccga
    11161 tggtgacgaa ggtgatggtg cgctcttcag ccaggccgag cgcccccatt ttggctgtca
    11221 ggcccggctg cagcttgagg gtatagttct tgttacgcgc aagaggtgcg gcgggcgtgg
    11281 cggcaataac gcgggaggag tcatcgccga ggcgccaatg gcgatgcagc tcttcgaagg
    11341 tggcatggcg gaagcgcagc ggcacggtgc gggcgccgtc gctcaggctg atgcggccgc
    11401 tgacggtggc gggatccatc tcgaggttga agcagaggaa aaaggaagcg cggggatcga
    11461 accccccctc gccgctctcc ggcgccgtat agagcagcac cgggcgcaga gtctcgaaac
    11521 tccaaacata gggggccttc aggacggcgc cgctaagggc cttggtcccc gccgggatgg
    11581 tcatctcgaa gcgggtcgcc gtgggcaggg tgtccagagg ggtaaagacc agggtgcggg
    11641 tgcccatcca gcgatacttg cccttgacgg ccggggagat ccttaacggc ccggaggcga
    11701 gaccctccgg cgtcgccttg agcgggacca tgggctcact gaaggtgacg acgatgctga
    11761 gggcatcggt gaccgccccg accgggcctt tgggggtgac ggattggacc tgcagatctc
    11821 cggccagggt gacaccggtg agggccagca tcagaaaaac gagactccat cctttcatca
    11881 aaacctccac tcgatttcaa atcccacccc ggacgcagaa ctgaccgcct ggccgcgttg
    11941 ggaggaaggc caggcgcgat aaggcttttt ctgagtttag caagatttcc agccatttgc
    12001 aagcatttgt tgactggttt tccaggcgcc gcctattacc tcgagaacag gatttttcag
    12061 accgcaccgc tgtggtgcat tcttatgctt tcaatccgtt tcaataccgg aggatgggaa
    12121 tggtgaagaa aaacctggaa gggatgaggg gtgagatggg atagattgtc ccgggagagc
    12181 tttttcagcg ccgtgatcag cgcctcgccc agtcccgagg tcccggctgc aaaacggtcg
    12241 gcctcgaact cgtggcggcg cgagaggaga ttcatcagcg gtccgaggag catctcgacc
    12301 ggcgagtaga gcagtccaaa aaagagcaga ccggcgtaaa gcgagatctg ctccatgccg
    12361 aaagcctcgt gcagtccggc gaggcgaatg aagagcgaca acagaaaaaa gatgatcccc
    12421 atgtgcgcga tgctgatcag catcccggtc aggatgtgcc tcttcttgta gtggccgatt
    12481 tcgtgcgcca gcaccgcgac cagctcatcg gtggcatgct tttcgatcag ggtatcgaag
    12541 agggcgatac gcttgttgcg gccgaagccg gtgaaaaagg cgttcgactt gttcgaacgc
    12601 ttcgatccgt ccatgacaaa gacccccttg aggggaaatc gcaccttctc tgcataggcc
    12661 aggatcgcgc ttttgagttc accctctgcc aggggcgtga acttgttgaa gaggggcatg
    12721 atccaggcgg gggcgacaaa gctcaccagc agtgaatagc ccgcagtaac cagccacgca
    12781 taaaaccagg cccactcccc cgcatgacca aaaaaggcca gcaatcctgc cagcagcgga
    12841 ccgccgagca gcacggagag cgccagcccc ttgagcaggt cgagcacgaa ggtcctgacc
    12901 gtggtgcgat tgaagccgaa acgctcctcg atgacaaagg tgtcatagat ggaaaaaggg
    12961 agggagagca ggctggagcc cagcagaagc aggccgatgt agagcagacc gctgaccaga
    13021 ggggaaaagc cccagccgcg cacaagcaga tcgagccagt tgaacccccc ggcgaaccag
    13081 aagagcagca gcaccgccag atcgaaggtg gcggtgatga agccgaacct ggtgttggcg
    13141 cgggtgtatt cctgcatgcg ggcataatcc cgggcgtcat agaccccctc aaattcagcc
    13201 ggcagggggt cgccgagggt gcgaagattc agcaccctgg cgagaatgtt gagggcatag
    13261 ctgatcagca gcgcgcccag gatgaccagg ccatatatat tcatcgtccc gaaaaccttt
    13321 ccatgacctc cactgcctct gctcccggcc ttgcacggtc gggagcgatc cctccggccg
    13381 acagctgctg gccgaat
//
LOCUS       DAOPWU010000060        13268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4769_length_13268_cov_5.296072, whole genome shotgun sequence.
ACCESSION   DAOPWU010000060 DAOPWU010000000
VERSION     DAOPWU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13268
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4769_length_13268_cov_5.296072"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="PKM23_04995"
     CDS             <1..1764
                     /locus_tag="PKM23_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90863.1"
                     /translation="CGGAERETVVKFGTAAAIPQNRSTGKLYIPPSEDDNRVTIEPSR
                     RAVLFEALHSQACSLQGLYPAPDQADGFGLEKRLCIPAGGAVTLCYLNILAETPAEAF
                     RQFDDLANEQIGRLFYTHREAWDRQIAAAFTPGNSEYSGFLPTLHTRDEAISRLYYTC
                     ALGLICFRRDNPHSVYGRAYDTLMPRYWQTTTFIWDYFISGGAHALLDPDVLRKYIGL
                     WMGTDLHSHFGTEYLNGGTVGPWYAVNDYALISLADDYLRWNGDFAWLESVPTAPTPA
                     AGESPAAPAEHKVIDFLREFAFNYRRFRTPSGLADYGGINNLLECVSTYIHEVASLNA
                     GVIYGLRTLAEILARQYRDPGAGACLAEAEELLQEVMKLYAAGGGYWNARFPDGALVE
                     VRHCYDFMTILNTLAGDLSGQQKEEMVRFFVGELQTPLWMRALSPRDPNALFSVRPDH
                     QWNGAYTAWPALAAAGLYRIGRADIAFPWLQGVARSANQGPFGQCHFVESAMPTESGG
                     ARKVFYEWPYGSDWACSSCGSYLTAIIEGIFGVRASKFDGISAAPQFGGFDPGAELHN
                     LRYREKRYNVSRKGLTQISGS"
     gene            1766..3007
                     /locus_tag="PKM23_05000"
     CDS             1766..3007
                     /locus_tag="PKM23_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNY90864.1"
                     /translation="MKKIILILIATIMTGCAMQKKELSPFDRFLAEVGKAGSPAQKQA
                     LAEAFYASLQPSTYPIFPDDTTYIMIFKGEKDSVGVLGDMNNWTQPDWMTRIAGTDLF
                     YSRGKAPVTARLEYWFVFGKNSLWAVDPLNPAKALNGFGELSELAMPGYEQHPFFAEY
                     RSGCKGSPEQLKVHEIDSRALGYSHTLHVYVPSGSSPAGGFPVLYLQDGLDYVEFAQV
                     PQMLDQLIASGAVQPLIAVFVTPPNRFQPGMPNRMTEYGMNEEYVRFFADELVPFIEA
                     RYPARRSPDARLVAGDSFGGLISAFIPFMRPEVFGNGCSQSGYVSFRGDSLIKLYRET
                     SRRPIRLFVDVGTYEENVGASFLPAAETNFTEGNRRFNKVLREAGYDFVYREYPEGHT
                     WGNWRRHLIDALIWFFPGEKP"
     gene            3004..3903
                     /locus_tag="PKM23_05005"
     CDS             3004..3903
                     /locus_tag="PKM23_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNY90865.1"
                     /translation="MRIGIDFGGTNVKFGLFDEEGNTLKFAVERVADLAAAGSLLETL
                     LDAAARFAAGEKLSRGGLGMKGMVDVRAGGVINDVGAGNEFAGIDLRARFAARLKVPF
                     AFDNDARAYAWGEWRFGAGRGCPSIIAMTLGTGVGCAAVIDGRLFRSSNPASGLLGGH
                     MSIDRNGPECPCGLKGCLELYCSAVNFEKQLLAAFPELTAETIPLKAFFAGAGARPAW
                     AAIKREFIDNLAIGVINVMHAYGVNTVILGGGLMKSHEQIIPGLLEIVKRRAWMVPKG
                     NVRILPALLEDRAACLGAAFLDE"
     gene            3957..5807
                     /locus_tag="PKM23_05010"
     CDS             3957..5807
                     /locus_tag="PKM23_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNY90866.1"
                     /translation="MMNSDDGELTPFAAPLPADAAAYPKGHYDTLPAFALAEGEINKG
                     YGELAALIQDKIPQGLRVLVIDGFPGVNWDGFLAGLQAELLHREIRAEFYHLAACLAY
                     PTNREEVFQPFLGGKDRLFGKHWPFGPEIFFDPLLVEELRITASIRRSDKAGRITIFA
                     GSGASLIELWDELWYIDLPKDVIQERFRKGRAGNIGYLADNFENFYKRCYFVEWPAFS
                     RLKRAILPRIDLFIDGQDDDNPAWMTGAAFRAALEEISRSPFRVRPWFYPGPWGGQFM
                     KGHMNLDQSKPNYAWSFEMIVPENGIVFESGGRFLECSFDFVMFAHNRSILGPRAAAQ
                     FIYEWPIRFDYLDTVDGGDLSVQVHPRPDYIKKEFGETYTQDECYYIVNAKPGARVYL
                     GLKEHTDPGGLKKALLHSEATGEELDVEAWIHSEPAQPHDLFFIPNGTVHCSGRGNLV
                     LEISATPYIFTMKIYDYLRRDLEGKLRPINIERGFANIRPERRGEWISRNLVARPRLI
                     EETEESRLYELYNQPWTFYVVQRAEFATRHTLLTEGRGFAVNLVEGERVEVHSANGRV
                     TSLAYLETMLIPAAAEAVTFVNRGSLPCRLVLVYVKPEAGLTVPVNDPND"
     gene            6081..7571
                     /locus_tag="PKM23_05015"
     CDS             6081..7571
                     /locus_tag="PKM23_05015"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNY90867.1"
                     /translation="MENKKRLLVFIAMIFLLLLRQGRGNNIQIQNVTIASWEMNEDYV
                     LVQFDLSWENSFRDEVNWDAAWVFVKYQLQGSSEWQHAWLGTTAAEHTVPAGYSCTVG
                     STTIDTINRGMGVFIYRSANGSGNTSLADVQIRWVYGANGVADGAPLTLKVFAIEMVY
                     VPQGPFYLGDADADLYYNFYTYGTSGPYLVTGEGEIPLGQTTGYLWAPNYVEAGALPA
                     AFPKGYDAFYCMKYEISQGQWVDFFNTLADSQKTTRDMTSYVAPLWGKYSDNVEWRNT
                     ISIISGFATCTTPDRTCNYLCWSDGTAYADWAGLRPMTELEYEKACRGTLPVVDDEFA
                     WGSADIIGAAAISGDEDGTEIISTAGANCRYNYIPLAGGDGGDGPLRGGIFARSATTR
                     EAAGSSYYGIMELSGNVWELAVNISNAAGKGYTGLHGDGSLDDTGMSDVANWPVYNST
                     AISYRGGAWDGDASAQRVSNRYLAGLPSFDRDGRNGFRCVRTAPSS"
     gene            7597..8925
                     /locus_tag="PKM23_05020"
     CDS             7597..8925
                     /locus_tag="PKM23_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY90868.1"
                     /translation="MANRSKFIIALWAVLCAPCLAFSQINETKYHGGSYDGHTSALFT
                     FAADRMDIPLSSGWSMISSNVTPYDPELSNLLASVSPNMVLMKDVSGAVYWPAQNITT
                     LDEWGIKNGYLIYLTARDTLVIRGEPVVPEITPIGLSSGWNMISFLNTRSITPGEALS
                     TVSSYLVIIKNSDGEVYWPAYNINTLGMMRPGSGYLAYVNQACVLTYPANLSLPKSSA
                     AAPATTISPAHFAGFRAKTPDNAIVLVTAHGLPDGAEIGAWTPSLLIGAGVVQMGRAL
                     LTLWGDDPLTVEVDGARTGESITLTCWDPFSAREQNVEYAKLTDGLTGTILPPLPAFQ
                     NNAVWHAELNAVPGQPEDFRLAQNFPNPFNPATAINFQLSNEAKVELMVINLSGMHVR
                     TLAAGNKPAGFHQVIWNGRDDDNRPVPSGIYWAKLKAGDFTKTIKMILIK"
     gene            complement(9166..10359)
                     /locus_tag="PKM23_05025"
     CDS             complement(9166..10359)
                     /locus_tag="PKM23_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY90869.1"
                     /translation="MQQQLFLSAGWNIVSSYVYPADPFLTEMLAGIEEHLLILKDGRG
                     RVFWPAYGINKIVFWEKEKGYQLYVTEAETLIVDGEQLVPEEVSIDLAAGWNMISYVR
                     SSPLAPAAALASITDYLFIVKDGEGRIYWPSQNIDQIGEMEPGKGYYCYLSQPAVLIY
                     PPNGAESGRISTPCSRQFPAPLHFHPFRTNTGSSAILLLKTPELLDGDEIAACTADGK
                     ITGSGVVQEGEALLVLWGDDEITPLVDGARNDEPLYLLCWFSRTGSELPVQCTSLRDA
                     LESKWSLVALRFHDNAVLTGETLASARLPERAALAQNYPNPFNSSTLLLYQLPENGEI
                     ELAVYDLCGRRVRSLAAGMKAAGWYEVVWDGRSDAGTPLSSGFYWVVMRTGGLRQQIK
                     MTLIK"
     gene            10604..10747
                     /locus_tag="PKM23_05030"
     CDS             10604..10747
                     /locus_tag="PKM23_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90870.1"
                     /translation="MSVKLLALLDGGHTLTNEDGNEILAWVRVDFYVLSKDLNVRGVV
                     NLQ"
     gene            11011..>13268
                     /locus_tag="PKM23_05035"
     CDS             11011..>13268
                     /locus_tag="PKM23_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNY90871.1"
                     /translation="MQIKEILVVHHSHLDVGYTHTQPIVWELHREFIDQALELLESTS
                     GWDERSAPKWTIEVTAQAEKWLESADSAAIDRLKGFITQGRVGLSGLEFNTTPLCTAE
                     QLIKQLEPVKKLRVLLGAEIRTANQHDVDGIPWSAADLLADAGIELLIMAVNIHLGGP
                     VAKRPSVFRWQGPSGREILVMNGAHYTMFDQLLYTWENSIARMQEGLARYLDHLAAMN
                     YPHDFIYLTTAATPVCWDNSPPYLEVAKLIREWNTRGLQPPIRFVTPNGLLERIQRRP
                     RGEYELLRGDWTDYWNFGCASTADVTRISRRAVQKLFKADLLEGFAPAAARHYSSLAA
                     STWRQLNLFDEHTWGSFNSMDPDNDFSKAQAAIKDHHAWQGHEIAEYLLVDRLEAFTG
                     NAADADRQEGIAVLNFSGRPVQEFVRIPEAWRAEGKRLRTARFGWDARTNQPAAAPLH
                     GPVALPPWGWAFIPFAVLHPAEPDPSLQEGEIVHESAARRLNTLEKTEEREVMRFIES
                     AYYRLEFNPATGRITRLFDKTNQRELLDGSSPYTFFQFVRERTDPLFREDRRAYYARE
                     LEKEQYDISCWNTEWRRLLETASKCLGCEVERTAEGITLILHFAAPGVENFTQRIMLH
                     VLKPLITLTAAFHKQEVRTPESLYFAFPLRLAPEWRCHFDTAGTVVELDAEQLPGASR
                     DWFTVESFVALHEKEYGVGLFCPDAPMIQAGGFNFGRRSKTIDRSANPLLLAWPLLGE
                     RLDWGSIGFALAV"
BASE COUNT         2640 a         4057 c         3801 g         2770 t
ORIGIN      
        1 tgcggggggg cggagcgcga aaccgtggtc aagttcggca ccgccgccgc catcccgcag
       61 aaccgcagca ccggcaagct ttatatcccc ccctccgagg atgacaaccg ggtcaccatc
      121 gaaccctcaa ggcgggcggt gcttttcgag gcactgcaca gccaggcctg ctccctgcaa
      181 gggctctatc ccgcgcccga tcaagccgac ggtttcgggc tcgaaaaacg gctgtgcatc
      241 cccgcgggcg gggccgtcac cctctgctac ctgaatattc ttgcggagac cccggccgag
      301 gctttccgcc agttcgatga tctggccaac gagcagatcg gccgcctctt ttacacccac
      361 cgtgaggcct gggaccgcca gattgccgcc gccttcaccc ccggcaattc cgagtattcg
      421 ggttttctcc ccaccctcca cacccgggat gaggcgatct cccgactcta ctatacctgc
      481 gccctcgggc tgatctgttt tcgccgcgac aatccccact cggtctacgg ccgcgcctac
      541 gataccctga tgccgcgcta ctggcagacc accaccttca tctgggacta tttcatcagc
      601 gggggcgccc atgccctcct cgatcccgac gtcctgcgca agtatatcgg gctctggatg
      661 ggcaccgacc tccactccca tttcggcacc gagtatctca acggcgggac cgtcggcccc
      721 tggtacgcag tcaatgatta cgcgctgatc tcgctcgctg atgactattt gcgctggaat
      781 ggggattttg cctggctgga gagcgtaccc acggcgccca caccggcagc gggggagagt
      841 ccggccgcgc cggcggagca caaggtcatc gacttccttc gcgagttcgc tttcaactat
      901 cgccgcttca ggaccccatc cggcctggcc gattacggcg gcatcaacaa cctcctcgag
      961 tgcgtcagca cctatatcca cgaggtcgcc agcctcaatg cgggcgtcat ctatggcctg
     1021 cgcaccctgg cggaaatact cgcccggcag tacagagacc ccggagccgg tgcctgcctg
     1081 gccgaggcgg aggaactcct gcaggaggtg atgaagctct atgcagcggg cggcggctac
     1141 tggaacgccc gctttccgga cggcgctctg gtcgaggtgc gccactgcta cgatttcatg
     1201 accatcctca acaccctcgc cggcgacctc agtgggcagc aaaaggagga gatggtccgc
     1261 ttttttgtcg gggagctgca gacccccctc tggatgcgcg ccctctcgcc ccgcgacccc
     1321 aacgccctct tcagcgtgcg cccggaccat cagtggaacg gggcctatac cgcctggccg
     1381 gccctcgcgg ccgccgggct ctaccgcatc ggccgcgccg atatcgcctt cccctggctg
     1441 cagggggtgg cgcgctcggc caaccagggg cccttcggcc agtgtcattt cgtcgaaagc
     1501 gccatgccca ccgagtccgg aggggcgcgc aaggtctttt atgagtggcc ctatggctcg
     1561 gactgggcct gctcctcctg cggcagctat ctcaccgcca tcatcgaggg catattcggg
     1621 gtgcgggcct cgaaattcga cggcatcagc gccgctccgc agttcggcgg tttcgatccc
     1681 ggggccgaac tccacaacct caggtaccgg gagaagcgct ataatgtctc ccgcaaggga
     1741 ctgacccaaa tcagcggatc atgaaatgaa aaaaatcatt cttattctca tcgccaccat
     1801 catgacgggc tgtgccatgc agaaaaaaga gctctctccc ttcgaccgct tcctcgccga
     1861 ggtcggcaag gccggcagcc cggcgcaaaa acaggcgctg gcggaggctt tctacgcgag
     1921 cctccaaccc tcaacctatc cgatcttccc ggatgatacc acgtacatta tgatctttaa
     1981 aggggaaaaa gattcagtcg gggttctggg cgacatgaac aactggaccc aaccggactg
     2041 gatgacgcgg attgccggga ctgatctctt ttattcgcgc ggcaaggccc cggtcaccgc
     2101 ccgcctcgag tactggttcg tctttggtaa aaacagcctc tgggccgtcg atccccttaa
     2161 tcccgccaag gccctcaacg gcttcggcga gctctcggag ctggccatgc ccggctatga
     2221 gcagcacccc ttcttcgcgg agtaccgcag cggctgcaag ggcagccccg aacagctcaa
     2281 ggtgcatgag atcgattccc gggcgctcgg ctattcccac accctccatg tctatgtgcc
     2341 atcaggttca tcgcccgccg ggggattccc cgtactctat ctccaggatg gactggatta
     2401 tgtcgaattc gcccaggtgc cgcagatgtt ggaccagctc atcgcctcgg gcgccgttca
     2461 gcccctcatc gccgtttttg tcacgccccc caaccgcttc cagcccggca tgcccaaccg
     2521 catgaccgaa tatggcatga atgaggagta cgtccgtttt ttcgcggatg agctcgtccc
     2581 cttcatcgag gcccgttatc ccgcccgccg ttctcccgat gcgcggctgg tggcaggcga
     2641 ttcgttcggc gggctgatct ccgccttcat ccctttcatg cggccggagg tcttcggcaa
     2701 cggctgcagc cagtccggtt atgtttcctt ccggggcgat tccctgatta agctgtatcg
     2761 ggaaacatcc aggcgtccca tcaggctttt tgttgatgtc ggcacctatg aggagaacgt
     2821 cggcgcttcg ttcctgcccg ccgccgagac caatttcacc gagggcaacc gccgtttcaa
     2881 taaggtgctg cgggaggccg gctacgattt cgtctaccgc gagtaccccg agggccacac
     2941 ctggggcaac tggcggcgcc atctcatcga cgccctgatc tggtttttcc ctggggagaa
     3001 gccatgagga tcggcatcga tttcggcggc accaatgtca agttcggtct cttcgatgag
     3061 gaggggaaca cgcttaaatt tgcggtcgag agggtggccg atctggccgc ggcgggctcc
     3121 ctgctggaga ctttgctcga cgcggccgcc cgcttcgccg ctggcgaaaa gctgagccgc
     3181 ggcggcctcg gcatgaaagg gatggtcgat gtgagggccg ggggggtgat caacgatgtc
     3241 ggcgccggca acgaattcgc cggcatcgat ctgcgcgcgc gttttgctgc gcggctaaag
     3301 gtgcccttcg ccttcgacaa cgacgcccgc gcctacgcct ggggtgagtg gaggttcggc
     3361 gccggacggg gctgcccctc gatcatcgcc atgaccctcg gcaccggcgt cggctgcgcc
     3421 gcggtcatcg acggccgcct cttccgctcc agcaatcccg ccagcggcct cctcggcggc
     3481 catatgagca tcgaccgcaa cggcccggaa tgcccctgtg gactcaaggg ctgcctcgaa
     3541 ctctactgct cggcggtcaa tttcgagaag cagctccttg ccgcctttcc cgagctgacg
     3601 gccgaaacca tccccctcaa agcctttttc gccggcgccg gcgcccgtcc tgcctgggcc
     3661 gccataaaac gcgagttcat cgacaacctc gccatcggag tgatcaacgt catgcacgcc
     3721 tacggcgtca ataccgtcat cctgggcgga ggactgatga agagccatga acagatcatc
     3781 cccggactgc tcgagattgt caaacgccgc gcctggatgg tgcccaaagg caatgtgcgc
     3841 atcctgccgg ccctgctcga ggaccgcgcc gcctgcctcg gtgccgcctt tctcgatgag
     3901 tgaggtcctg gctgattttc cgctctgtgc cgatgaatga atgcaaagga attaccatga
     3961 tgaatagtga cgatggggaa ttgacccctt tcgcggcccc cctgccggcc gacgcggcag
     4021 cctacccgaa aggccattat gacaccctgc ccgcctttgc actggcggag ggggagatca
     4081 acaagggcta cggtgaactc gccgccctga tccaggataa aatcccgcag ggtctgcgcg
     4141 tgctggtcat cgacggcttt ccgggtgtca actgggatgg ttttcttgcg ggactgcaag
     4201 ccgaactgct gcaccgggaa atccgcgccg aattttacca ccttgcagcc tgtctggcct
     4261 atcccaccaa ccgcgaggag gtgttccagc ccttcctcgg cgggaaggac cgcctcttcg
     4321 gcaaacactg gcctttcgga ccggagatct tttttgatcc gttactggtg gaggagctgc
     4381 gcatcaccgc ctcgatccgc cgcagtgaca aggccggccg gatcactatt ttcgccggca
     4441 gcggagcctc cctgatcgaa ctctgggacg aactctggta tatcgatctg cccaaggatg
     4501 ttattcagga gcgtttcaga aaaggcaggg ctggcaacat cggctatctc gccgataatt
     4561 tcgaaaattt ctacaagcgc tgctatttcg tcgagtggcc cgccttcagc cggctgaagc
     4621 gagcgatcct gccccgcatc gatctcttca tcgacggcca ggatgacgac aatcccgcct
     4681 ggatgaccgg cgccgccttc cgcgccgccc tggaggaaat ttcgcgctcg cccttccggg
     4741 tgcggccctg gttctacccc gggccctggg gcgggcagtt catgaaaggc cacatgaatc
     4801 tcgaccaaag caaacccaat tacgcctggt ccttcgagat gatcgtcccg gaaaatggca
     4861 tcgttttcga gagcggcggc cgttttcttg agtgctcctt cgattttgtc atgttcgccc
     4921 acaaccgcag catcctcggc ccgcgcgcgg ccgcacagtt catttatgaa tggccgatcc
     4981 gctttgacta tctcgacacc gtcgacggcg gcgacctctc cgtccaggtt catccccggc
     5041 cggattatat caaaaaggag ttcggggaga cctacaccca ggatgagtgt tactacatcg
     5101 tcaacgccaa acccggcgcc cgggtctatc tcggcctcaa ggaacatacc gatccggggg
     5161 gactcaaaaa ggccctgctc cactcggaag cgaccgggga ggaactggat gtggaggcct
     5221 ggatccacag cgagcccgcg cagccgcacg acctcttttt catccccaac ggcaccgtgc
     5281 actgcagcgg ccgcggcaac ctggtgctgg agatcagcgc caccccctat atcttcacca
     5341 tgaagattta tgactacctg cggcgggacc tggagggcaa gctgcggccg atcaacatcg
     5401 agcgcggctt cgccaacatc cggcccgaaa ggagggggga gtggatcagc cggaacctgg
     5461 tcgcccggcc caggctgatc gaggagactg aggagagccg cctctatgaa ctctacaacc
     5521 agccctggac cttttatgtc gtgcagcgcg ccgaattcgc cacccgacac accctgctta
     5581 cggaggggcg gggcttcgcc gtcaacctcg tcgaggggga aagggtggag gtccattctg
     5641 ccaatggccg cgtcacctcc ctggcctatc tcgagaccat gctcatcccg gccgctgcgg
     5701 aggccgtcac ttttgtcaac cggggcagtt tgccctgccg gctcgtcctc gtttacgtca
     5761 agcccgaggc cggcctcacc gtgccggtca acgatcccaa tgattagcga tgaagcgtcc
     5821 actcgatggc tgcggttgcg gtcactcttg gggatcactc tgatggctga tgtgaatatg
     5881 caaagagagg agtatgtcca aatttaaaac acttttatat tgcattattg gcgcattatc
     5941 gatatttttt tatagtactg atgatataga cgatcagtcg tattattaac gggggttaat
     6001 ctgacacctt cgatgagcac agataagatg ctactctttt ctgtgctttt tttgtttttg
     6061 ggagctaatc agagggaaat atggagaata aaaaacgtct attggttttt atcgccatga
     6121 ttttcctgtt actgttgagg caggggcgtg gcaataacat ccagatacaa aacgtgacga
     6181 ttgccagttg ggagatgaac gaggactacg tcctggtcca attcgatctt tcctgggaaa
     6241 acagcttccg cgacgaggta aactgggatg ccgcctgggt ctttgtcaaa tatcagctcc
     6301 aggggagtag tgaatggcag cacgcctggc tgggtaccac ggcggccgaa cacaccgtcc
     6361 cggctggata ttcctgcacg gtgggaagca caaccattga tacaatcaac agagggatgg
     6421 gcgttttcat ctaccgcagt gccaacggga gtggtaacac aagtctggcc gatgttcaga
     6481 tacggtgggt atatggagcc aacggtgtcg ctgatggcgc cccgttgaca ctcaaggttt
     6541 ttgcgataga aatggtttat gtacctcaag gaccatttta tctcggcgat gcggatgcgg
     6601 acctttatta taatttttac acctacggca cttctggtcc atatctggtt actggagaag
     6661 gcgagatccc gcttggtcag acgaccggct acctctgggc tcctaattat gtagaagcag
     6721 gcgcgctccc ggctgcattt ccaaaggggt acgatgcatt ttactgtatg aagtacgaaa
     6781 tcagccaagg ccagtgggtt gatttcttca acaccctggc ggactcacaa aagaccacca
     6841 gggatatgac ttcctatgta gctccattat ggggcaaata ttcggataat gtagagtggc
     6901 gtaatacaat ttcaatcatc tctggtttcg ccacctgcac cactcccgac cgcacatgca
     6961 actatctgtg ctggtccgat ggcacggctt atgcggattg ggctggatta cggcctatga
     7021 ctgagctaga gtatgagaag gcgtgccgcg gcactctgcc ggttgtggat gatgaattcg
     7081 cctggggatc agccgatatc atcggcgcgg ctgccatcag tggagatgag gatggaacgg
     7141 agatcatttc tactgcaggt gccaattgcc gatataatta catcccgctc gcaggtggtg
     7201 atgggggaga cggcccgctg cggggtggaa tttttgccag atcggcgacg acgagggaag
     7261 cggctggctc ttcatactat ggcattatgg agttgtccgg caatgtctgg gaattggctg
     7321 taaacatctc aaacgcggca ggcaaagggt acaccggtct tcacggtgat ggttcgctgg
     7381 atgacacggg tatgtccgac gttgcaaatt ggccggtcta taattctaca gcgatcagct
     7441 atcggggcgg ggcttgggat ggtgatgcct ccgcccaacg ggtctctaac cgctatcttg
     7501 ccggtctccc gagttttgat cgggatggcc gcaacggttt tcgttgtgtt cgtacggcgc
     7561 cttccagcta aatcgttaag gctttggagg ttttctatgg cgaacagatc aaaattcata
     7621 atagcgctat gggccgtact ttgcgcccca tgtctggctt tttcccaaat caatgaaact
     7681 aaataccatg gcggctctta tgacggtcat acctctgcgc tctttacctt cgcagctgac
     7741 aggatggata tcccgctcag ttccggctgg agcatgattt caagcaatgt gacaccgtat
     7801 gatccggagt tgtcaaatct gcttgccagt gtttcaccca atatggttct gatgaaggat
     7861 gtaagcggcg cggtctactg gcctgcgcag aacatcacta cgcttgatga gtggggcatc
     7921 aaaaacggat atctgattta tctcacagca cgagatacac tcgtgatcag gggagagcct
     7981 gtggttccag agatcacgcc gattggtctc tcatccggct ggaatatgat ctcttttctg
     8041 aatacccgat caatcacacc gggggaagcg ctctcaactg tctcatccta tctggtgatc
     8101 attaaaaaca gcgacgggga ggtttactgg cctgcctata acatcaatac attaggcatg
     8161 atgcgcccag gctcggggta tctggcctat gtcaatcagg catgcgttct gacctatccg
     8221 gccaatttgt cactgccaaa aagtagtgcg gcggctccgg cgactacaat ttcaccggct
     8281 cattttgctg gatttcgtgc caaaaccccc gataacgcca tagttctggt cactgctcat
     8341 ggtctgccgg atggagcgga aatcggtgcc tggaccccca gcctgctgat cggcgccggc
     8401 gtggtccaga tggggagagc gctcttgact ctgtgggggg atgatccgtt gacggtcgaa
     8461 gtggacggcg ccagaaccgg tgaatccatc acactcacct gctgggaccc tttttcagcc
     8521 agagaacaga atgtggagta cgccaagctg acagacgggc tgaccggcac catcttgccg
     8581 cccttgccgg cttttcaaaa caacgcagtc tggcatgcag aactcaatgc ggttcccggg
     8641 caaccggagg attttcggct tgcacaaaac ttccccaatc cttttaatcc ggctaccgcc
     8701 atcaactttc aactgtcgaa tgaagcaaag gttgagttga tggttatcaa cctttcaggc
     8761 atgcatgtgc gcacgctggc agcaggtaac aaaccagcag gatttcatca ggtcatctgg
     8821 aatggtcggg atgacgacaa ccgacctgtt ccttcgggaa tttactgggc aaaactcaaa
     8881 gctggagact ttacgaagac gatcaagatg atattgatta aatgatattc accaaagtag
     8941 atacggcgcg atctgcagag gcatggcagg aaaaggtgtt tgatctgctg ctgtgtcagg
     9001 aaaagatcgt tgaggatgtg gtacgatcga tgcggggatg gcctggtaat cggggcttgc
     9061 cagagactga tttactcgat tcctttgccc ccggcaagat cacatttcct gtcaatcagc
     9121 agccccactg gcggggtcga ttgctccggg gggtgaggga tgccattatt tgatcagcgt
     9181 cattttgatc tgctgccgta aacctcccgt cctcatcaca acccagtaga atcccgagga
     9241 gaggggcgtt cccgcatcgc tgcgtccatc ccagacgact tcataccagc cggctgcttt
     9301 catccccgcc gccagagagc gcaccctgcg gccgcacaga tcatagacgg ccagttcaat
     9361 ctccccattc tccggcagct ggtagagaag cagggtcgag gaattaaagg ggtttggata
     9421 gttctgcgcc agggcggcgc gctcaggcag tcgggcagaa gccagagtct ctccggtcag
     9481 cacggcattg tcatgaaaac ggagcgccac caggctccac tttgactcca gggcatcccg
     9541 gagcgaggtg cactgtacgg gcagttcgct gccggtgcgg gaaaaccagc agagcagata
     9601 caggggctca tcattgcgcg ccccatccac cagcggagtg atctcgtcat caccccaaag
     9661 caccagcagt gcctctccct cctgcaccac gccgctgccg gttatttttc cgtccgccgt
     9721 acaggccgcg atttcatccc catccaacag ctccggcgtt tttaataaaa gaatggcgct
     9781 gctgccggtg ttagtcctga agggatggaa atggagcggc gcggggaact ggcggctgca
     9841 aggggtggag atcctccctg actccgcccc attgggcgga taaatgagca cggcgggctg
     9901 ggagagatag cagtaatagc ccttgcccgg ctccatttca ccgatctgat caatgttttg
     9961 cgaaggccag tagatcctgc cctcgccgtc cttgacgatg aacaaatagt cagtgatgga
    10021 ggcgagggcg gctgcgggag cgagcggact gctgcgcaca tacgagatca tgttccagcc
    10081 tgcagccaga tcgatggaga cctcctccgg caccagctgc tcaccatcga caatcagggt
    10141 ctccgcttct gtcacataga gctggtaccc cttttccttt tcccagaaga cgattttgtt
    10201 gatgccatag gccggccaga aaacccggcc tctgccatcc ttgaggatga gaagatgttc
    10261 ctcgatcccc gccagcatct ccgtcaggaa gggatcggct gggtaaacat agctgctgac
    10321 tatattccag ccggcggaga gaaacagctg ctgctgcacc cctgtcgcag cggctcctct
    10381 gccgcaggat tgcgcggtgg agccggcgcg ggaggagtcc tgagcgcgga gggatgcgat
    10441 gaacagcatc gacgtgcaga tagagatccg gacgaaagac cccatggggt gttccttgcg
    10501 gttataatct gagcagtgcg ggcccgctgg cgggcaagtc ccgtccataa aaaaaatacg
    10561 tgagatatgg agaagaggct gattaaatat aacccatccg gaggtgtctg tcaagctttt
    10621 agctttgctc gatggcggcc ataccctcac gaatgaagat ggtaatgaaa tacttgcgtg
    10681 ggtgcgggtc gatttttatg tcttgtccaa ggatttaaat gtgcgcgggg tggtcaatct
    10741 gcaataatac agtccttccc gggtttctgc gcaagcagat cctgcagcga ggtgcaattt
    10801 tgcattctca taatatatta tgcttgattt gcagatgtgg attgcctaat ttgccggcgg
    10861 gcataaagtg atgaggatgg ccggataggg cctggggctc tgtaaaaggg gaagaggccg
    10921 gaatccggcg cttggcagag actggtttac tcgctttctt tgcccccggc aaatttgtat
    10981 atcttgtcaa ttatcaattc ctgtcaatct atgcagatca aagagattct tgtcgtccat
    11041 cacagccacc tcgatgtcgg ctacacccac acccagccca tcgtttggga gctgcaccgc
    11101 gaattcatcg accaggccct cgaactcctc gagtccacct ccggctggga cgagcgttcc
    11161 gcccccaaat ggaccatcga ggtgaccgcc caggcggaga aatggctgga gagcgccgac
    11221 agcgccgcga tcgaccgcct caagggcttc atcacccagg ggcgggtcgg cttatccggc
    11281 ctcgagttca acaccacccc cttgtgcacg gccgagcagc tgatcaagca gctcgagccg
    11341 gtcaaaaagc tgcgtgtgct cctgggcgcc gagatccgca ccgccaacca gcacgatgtc
    11401 gacggcatcc cctggagcgc cgccgatctc ctcgccgacg ccggcatcga gctgctgatt
    11461 atggcggtca acatccacct cggtgggccg gtcgccaaac gcccttcggt tttccgctgg
    11521 caagggccct caggccgcga gatcctggtc atgaacgggg cccactatac catgttcgac
    11581 cagctcctct atacctggga aaactcgatc gcacgcatgc aggaggggct ggcgcgctac
    11641 ctcgaccatc tcgccgccat gaactacccc cacgatttca tctacctgac caccgcggct
    11701 acgccggttt gctgggacaa ttcgcccccc tacctcgagg tcgccaaact gatcagggag
    11761 tggaacaccc gcggcctgca gccgccgatc cgttttgtca ctcccaacgg cctgctcgaa
    11821 cgcatccagc gccggccgcg cggggagtac gaactcttgc gcggcgactg gaccgattac
    11881 tggaatttcg gctgtgccag caccgccgac gtcacccgca tctctcgccg tgctgtgcaa
    11941 aagctcttca aggccgatct gctcgagggc ttcgccccgg ccgccgccag gcactattcc
    12001 agcctcgccg cctcgacctg gcgccagctc aacctcttcg acgaacacac ctggggctcc
    12061 ttcaactcga tggacccgga caacgatttc tccaaggccc aggccgccat caaggaccac
    12121 catgcctggc aggggcacga gatcgccgag tatctgctcg tcgaccgcct cgaggccttc
    12181 accggcaatg cggcggacgc ggaccggcag gaggggatcg ccgtgctcaa tttcagcggc
    12241 cggccggtgc aggaattcgt gcgcatcccc gaggcatggc gcgccgaggg caaacggctg
    12301 cgcaccgccc gcttcggctg ggatgcccgc accaatcaac ccgccgctgc gcccctccat
    12361 ggcccggtgg cgctgccgcc ctggggctgg gccttcattc ccttcgcagt gctgcacccc
    12421 gccgaaccgg atccctccct gcaggagggg gagattgtcc acgagagcgc ggcgcgccgc
    12481 ctcaataccc tcgagaaaac cgaagaacgc gaggtgatgc gcttcatcga atcggcgtac
    12541 tatcgcctcg agttcaatcc tgcgacaggc cggatcaccc ggctctttga caaaaccaac
    12601 cagcgcgagc tgctcgacgg ttccagcccc tacacctttt tccagttcgt gcgcgagcgc
    12661 accgatcccc ttttccgcga ggatcggcgc gcctactacg cgcgtgagct cgagaaggag
    12721 cagtacgata tttcctgctg gaacacggag tggcgacggc tcctcgagac cgcttcgaaa
    12781 tgccttggct gtgaggtcga gcgcacagcc gaggggatca ctctgatcct gcacttcgcc
    12841 gcacccgggg tcgaaaattt cacccagcgg attatgcttc acgttttgaa gccgctcatc
    12901 accctgactg ccgccttcca caagcaggag gtgcgcaccc cggagtcgct ctatttcgcc
    12961 tttcccctgc ggctggcgcc ggagtggcgc tgccatttcg acactgccgg gacggtggtg
    13021 gagcttgatg ccgagcagct gccgggggcg agccgcgact ggttcacggt cgaatcgttc
    13081 gtggccctgc acgagaaaga gtacggcgtt gggctcttct gcccggacgc cccgatgatc
    13141 caggcggggg gattcaattt cggccggagg agcaagacga tcgatcgcag tgcgaatccc
    13201 ctgctgctgg cctggccgct gctgggcgag cggctggact ggggctcgat cggctttgcg
    13261 ctggcggt
//
LOCUS       DAOPWU010000061        13228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4785_length_13228_cov_5.434297, whole genome shotgun sequence.
ACCESSION   DAOPWU010000061 DAOPWU010000000
VERSION     DAOPWU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4785_length_13228_cov_5.434297"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="PKM23_05040"
     CDS             <1..1680
                     /locus_tag="PKM23_05040"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HNY90872.1"
                     /translation="ATRREFVKKSTQCCIGAGLLASAGAVITGCSRRPYDLLIVNGNL
                     YDGSGGAPVRADIGIRGDRIAAVGRLDDEGCPQVIDAKGLAVSPGFVDVHTHTDLHLL
                     AEPRAESKIHQGVTLEIGGNCGDSVFPLTSTGRREVQEEWRREYGIEAGWSDLAGFFE
                     VLGKKGSALNYATLIGHSTIRSVAMGAADRPPAGAELEAMKGHLLCALEQGALGLSTG
                     LEYTPGAFAATDEIIALCREVARHGKVYATHMRNEDLTVEEALEETLRIGRDSGVSVQ
                     ISHFKTCQQRNWHKTPKLLAAVEAARREGMNVHADRYPYTAYATSLKMLFPLWAREGG
                     NPAFVARLKDPRGFADMAPFVRDKIAATGSWASIMITQAWLSGRRDYQGKTVEELATA
                     AGEDPLDFTRRLLIEEEGQVGMCGFSMSEEDTRATFAAPYTMVGSDGNAISPSGLLGR
                     GTPHPRYYGTFPRYLGYYVREKKVLPLAEAIHRITALPCEKFSIRERGLIREGCFADI
                     VLFDPATVIDRATFVQPHQFPTGIDWVIVNGEIVVAEGEQTGKLPGRILRG"
     gene            complement(1735..3021)
                     /locus_tag="PKM23_05045"
     CDS             complement(1735..3021)
                     /locus_tag="PKM23_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY90873.1"
                     /translation="MNKTWVLIKREFLIRVKNKGFIIGTFALPVLIVVIMGIQIAVQS
                     MGAREKKTLALIDLTGEVGPAYTAYMDENFRDDRKVPLYTFETVTTVPESLAAVTARY
                     SLLVQQEKLSALIILPADIFLTSQFELHARNISNMEFNAALERSLSRVVSDLRLAESG
                     LDQELVRKLTQRMDLKTFKVSEAGTKEESGFATWGISYAMMFILYMALIFYGQFVMRG
                     VYEDKNSRVMEVVLSSVRADQLMAGKIIGIGGAGLLQFLIWALFVALASAYGMTMMNY
                     FAPGAGDFPLPTISFSVYLFFFIFFLLGYFIYATLYATLGSMVNDEADAQSLQWPVTM
                     LIVLGFLFMFFVLNNPNSTLAVILSLIPFFTPLLMFLRLSVGAAPMWQVLLSVVLMIA
                     SIIALIWFTGRIFRVGILMYGKKPNLPEVLKWIKYS"
     gene            complement(3014..3937)
                     /locus_tag="PKM23_05050"
     CDS             complement(3014..3937)
                     /locus_tag="PKM23_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY90874.1"
                     /translation="MRSAVTLNKVIKSFDTVRAVDNLSLEIPQGEIFGLIGPNGAGKT
                     TTIRMLLGILMPDSGTIEILGKSSCREVANQVGFLPEERGLFKRMVIIDIIRFFAELK
                     GIPPRKATPLEEIWLERFELKPWRDKKVEELSKGMQQKVQFITTILHEPRLIFLDEPF
                     SGLDPVNTNLIKDVMLDLVKNGTTIIFSTHLMEQAEKLCDALCLIDKGRSVLQGRLAE
                     VKGRFGRNRCRLSYDGEARFLKDTALVSRYDDYGQYVEIQPAEGVQPQAILAQAVSQV
                     TVRSFNITEPSLNEIFITVVKGGANAGGDHE"
     gene            4196..5044
                     /locus_tag="PKM23_05055"
     CDS             4196..5044
                     /locus_tag="PKM23_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNY90875.1"
                     /translation="MRWQGRTESENVEDRRGTTSRRVIGGGIGTLVILLLVWLLGGDP
                     MQFLQENPGVSTSTPVSTEERSAEENQLASFVKVVLKDTEDVWGKLFQNAGSQYRMPT
                     LVLYTDQVQSACGYGSAATGPFYCPGDEKVYIDLAFCAELQNQYDAPGDFAIAYVIAH
                     EVGHHVQNLLGITDQIASMQGRVSQEEYNQYSIRLELQADFFAGVWAHHAQKMNNILE
                     PGDLDEAINAASAVGDDRIMRRTQGYVVPDAFTHGTSDQRQRWFKKGWDTGDPNQGDT
                     FAARTL"
     gene            5151..7172
                     /locus_tag="PKM23_05060"
     CDS             5151..7172
                     /locus_tag="PKM23_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNY90876.1"
                     /translation="MRLLKGGFLFMAAILYAAAAAALELPAIFGDHMVLQQRQRVAFW
                     GKAAPGEKVGLEASWGARSAAIADNNGQWRAFLKTPAAGGPWTVTVTSPSGTKSFMDV
                     LTGEVWICSGQSNMEMPLAGWPPGDPIANSAAEIAAADFPQMRLFTVERAIAAAPVDD
                     CIGRWQPCTPQTAAAFSATAFFFGRTLLNELNIPIGLIHTSWGGTPAEAWTSAEYLKT
                     LPRYASVLEKVKTSSTQLEARKRWLQNHAVFPVLKSGSDRWVNLDFGDSMCPAYSYDD
                     SRWGRMQLPGSWEATEVGQFDGAVWFRKLVEIPAEWAGEELLLELGPIDDMDVTYFNG
                     ERVGGMEQDGLWQTERLYTVPAELVRSGANLVAVRVLDTQGGGGIYGRKEQLRLYPRS
                     KPDRAISLAGEWRYLPVAEYQNARFYQFDVAAADFFDRPKVEVEISANTPSFLYNAMI
                     APLVPYTLRGAIWYQGESNSGRPLEYRTLFPLMIDNWRRAWGGTPFPFYYVQIAPYDY
                     GAATPSEELREAQMLTLSHPKTGMAVTMDIAAPSIHPPNKQEVGRRLALWALDRDYGH
                     KQVHSGPIYRKMKIKGERIELTFDHSGSGLKAAAEGLTGFEIAGADKKFVPAAAVIDG
                     RRVVVSSTAVPKPVAVRYAWSNLARATLFNLEGLPASSFRTDNWQLSIN"
     gene            7192..8508
                     /gene="xylA"
                     /locus_tag="PKM23_05065"
     CDS             7192..8508
                     /gene="xylA"
                     /locus_tag="PKM23_05065"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNY90877.1"
                     /translation="MPKSYFPEIKGPIPFEGKGSKNPLAFRYYNKDQKVGGKTMAEHL
                     RFAVAYWHTMMADGTDMFGGASYDRAWHAPSDPIDRAKATMDAAFEFFTKLGVDYYCF
                     HDRDIAPAGESFAGSCKNLEIMVDYAKGLQKQTGVKLLWGTANLFGHRIFTAGASTNP
                     DAHVFALAAAQVKNAMDATLALGGENYVFWGGREGYESLLNTDLKHEQEQLARFFHMA
                     VDYAKSIGFKGQFLIEPKPCEPTKHQYDTDTATALAFLRQFDLIDYFKINVEANHATL
                     AKHTFEHELTVASAAGKLGSVDANRGDLLLGWDTDQFPTDIYSTTLAMLVILKQNGLG
                     SGGLNFDAHVRRSSIDPVDLFHAHIGGMDAFARGLLVAHRIIEDKALSSFIDERYVSY
                     KSGIGAKIMAGQVGFAELEKYILEKGMPVMRSGRLEMLENLLNDYI"
     gene            complement(8579..10525)
                     /locus_tag="PKM23_05070"
     CDS             complement(8579..10525)
                     /locus_tag="PKM23_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNY90878.1"
                     /translation="MKPFGKLIPAAVWLLLIFAAGSGFAEVRLPRLISDGMVLQRESR
                     VNIWGWARPGEKVNLEFRGRHYRTAADGEGRWLLRIRSGAAGGPFTMGIEGEDRITLK
                     DIYAGEVWLCSGQSNMALPIARVEERYPDLVAGADNPAIRHFFVPMRYDFNRPQEDLE
                     AGAWQGATAETVRGFSAAAYFFALALYEKYGVPIGLINASVGGTPVEAWMSEKALQAW
                     PVHLDSALRCRDESYVQGILAADQARIGSWFRLLRAQDAGYADPAASWADPDYDDSSW
                     PMMPVPSFWKERGLGPINGAVWFRREIDLPETMAGQPGRLILGAIVDADSVFLNGTFV
                     GTTSYQYPPRRYAVPAGLLKAGKNLLTVRVISNIGSGGFIKDKAYQLVVAGDTLDLSG
                     EWRCQVGAVMEPLAGQTFIQYKPLGLFNGMIAPLLNYRIKGAIWYQGESNTGRAQEYT
                     RLFPDLIRDWRQQFSQGKFPFLYVQLANFMVARTEPAESGWAALREAQRKTLALPNTG
                     MAVAIDIGEWNDIHPLNKRNVGERLALWARRLAYKDKELVPSGPLLRTSRVEGSKIIL
                     SFDHTGSGLAVRDGGELRHFAIAGADGRFVWAQARITGDRVEVWSEAISRPAVVRYGW
                     ADNPAGANLINREGLPASPFSTEK"
     gene            complement(10549..12177)
                     /locus_tag="PKM23_05075"
     CDS             complement(10549..12177)
                     /locus_tag="PKM23_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HNY90879.1"
                     /translation="MRKSAFSLILLTLLCSRGESVPKDGALAIPHLRQQGEAVQLIVG
                     GKPLLMLAGELGNSSASDAASMRWIWPKLKNMHLNTALVPIYWELIEPEEGRFEFALV
                     DSILQAARANDLKLVLLWFGTWKNSMSCYAPLWVKSDPVRFPRARTRAGEPQEILTPF
                     SAENLQADVRAFTALMRHLRKMDEKEQTVVMVQVENEIGMIPDARDHCREADKRFAEA
                     VPADLVAYMQKNKKTLHARLREAWEKQGSPVRGNWETLFGIGPATDEFFMAWHFARYT
                     DEVARAGKAEYPLPMYVNAALIRPGYQPGQYPSAGPLPHLIDIWRAGAPQIDFLAPDI
                     YFRNFREWAVQYDVPGNPLFIPEVGNDQSPANAFYAIGEHDAMGYSPFSVEDLADPGR
                     NPLSRAYALLRQLQPLLITHQGKGSMRGVLLDSTGQHARIEMGDYLLRIRHEYSWSYA
                     RREEGVMPRYGGLFLRLADDEFIVAGTGLIIEVAPRAEGVRAGIGSVEEGRMIEGVWH
                     SGRRLNGDQTHQGRHIQLPGQDYTMLRVKLYSYR"
     gene            complement(12293..>13228)
                     /locus_tag="PKM23_05080"
     CDS             complement(12293..>13228)
                     /locus_tag="PKM23_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNY90880.1"
                     /translation="GLVDGIGIQGHYFEFRSDTRSANQYVYSITTLKANLERIAAVGL
                     PIYITEFDIDEPDDAVQLAQYQIYFPLFWNQPAVKGITFWGYIEGDVWTSHPYTYLLK
                     ANGTERPVMPWLRDYIKKPISPLLVGPNGTLGELRNPLLLWHSTALADSYRVQLSTAR
                     AFTSIAMDTIVADTTFQLGKLAANSRFYWRVSALNTYGSSEFSAPAWFETGVQSSVEE
                     APARSPELFELAQNYPNPFNPATTIRFTLAAAGHVELKVHNLLGREMAILVSRTLEAG
                     THSFRFDATILKSGTYLYTLRTAGFTATRRMIILK"
BASE COUNT         2630 a         4006 c         4036 g         2556 t
ORIGIN      
        1 gcgacccgac gtgagttcgt caagaaaagt acacaatgct gcatcggcgc cggcctgctc
       61 gcttccgccg gcgctgtcat aacgggctgc tcgcgccggc cctacgacct gctcatcgtc
      121 aacgggaacc tctatgacgg cagcggcggc gcacccgttc gggcggatat cggcatccgc
      181 ggcgaccgca tcgccgctgt cggccgtctc gacgatgagg gatgcccgca ggtcattgac
      241 gccaagggct tggccgtcag ccccggcttt gtcgatgtcc acacccatac cgatctgcac
      301 ctcctggccg agccccgggc ggagagcaag atccaccagg gggtgaccct ggagatcggc
      361 ggcaactgcg gtgactcagt gtttcccctt acttcaaccg gaaggaggga ggttcaggag
      421 gagtggcgcc gggaatatgg cattgaagcg gggtggagcg atctggccgg ctttttcgag
      481 gttcttggta aaaaggggtc cgccctcaat tacgctaccc tgatcggcca cagcaccatc
      541 cgcagtgtgg cgatgggggc cgcagatcgg cctcccgccg gcgctgaact cgaggcgatg
      601 aagggccatc tgctctgcgc cctggagcag ggggccctgg ggctctccac cgggctcgaa
      661 tacacccccg gggctttcgc cgccaccgat gagatcatcg ctctgtgcag ggaggtcgcc
      721 cggcatggca aggtttatgc cacccatatg cgcaacgagg acctcaccgt ggaggaggct
      781 ttggaggaga ccctgcgcat tggccgcgac tccggggtga gcgtgcagat ttcccatttc
      841 aagacctgcc agcagcgcaa ctggcacaag accccgaaac tgctggccgc tgtcgaggcc
      901 gcccgccgcg aggggatgaa tgtgcacgcc gaccgctatc cctacaccgc ctacgccact
      961 tcgctgaaga tgctctttcc cctgtgggcg cgggaaggag gcaatcccgc ttttgtcgcc
     1021 cgtctgaaag accccagggg ctttgccgac atggccccct ttgtgcgcga caagatcgcc
     1081 gccaccggct cctgggcttc gatcatgatt acgcaggcct ggctctcggg aaggcgggac
     1141 tatcagggca agacggtcga ggaactcgca acagcagcag gagaggatcc cctcgacttt
     1201 acccgccgtc ttctcatcga ggaggagggc caggtgggga tgtgcggatt cagcatgagc
     1261 gaggaggata cccgggccac cttcgccgcc ccctacacca tggtcggttc ggacggcaac
     1321 gccatctccc cctcaggcct gctcgggaga gggactcccc atccgcgcta ctatggcacc
     1381 ttcccgcgct acctcggata ctatgtgcgc gaaaaaaagg tgctccccct ggcagaggcg
     1441 atccaccgca tcactgcgct gccctgtgaa aagttttcca ttcgtgagag ggggttgatc
     1501 agagaaggat gcttcgccga tatcgtcctc tttgatccgg cgacggtcat cgaccgggcc
     1561 acctttgtgc aaccgcacca gttcccgacg ggcatagatt gggtcatcgt caacggcgag
     1621 atcgttgtgg cggaagggga acagaccgga aaactgcccg gccgtattct gcgcgggtga
     1681 gggggcggaa gacccgcccc ctcatgcatt gttacatcag cctctgttca cgaatcagga
     1741 gtacttgatc catttcagca cctccgggag attgggcttt ttaccataca tcaaaatgcc
     1801 gacccggaaa atccgtccag tgaaccagat cagggcgatg atcgaggcga tcatcagcac
     1861 cacgctgagg agcacctgcc acatcggcgc ggcgccgaca gagagccgta aaaacatcag
     1921 cagcggagtg aagaagggga tgagggagag gatgacagcc agagtgctgt tgggattgtt
     1981 gaggacgaag aacatgaaga gaaagccgag cacgatcagc atggtcaccg gccactgcag
     2041 gctctgggca tctgcctcat cgttgaccat gctgccgagg gtggcataga gggtcgcata
     2101 gataaaatag ccgagtagaa aaaaaatgaa gaagaagaga tagaccgaaa aggagatggt
     2161 gggcaggggg aaatcacccg ctcctggggc gaaatagttc atcatcgtca tgccatatgc
     2221 cgaggccagg gcgacaaaaa gcgcccagat cagaaactgc aggagtcctg ctccgccgat
     2281 gccgatgatc tttcccgcca tgagctgatc ggcccggacc gaggagagca cgacttccat
     2341 aacccgcgag ttcttatcct catagacgcc gcgcatgaca aactggccgt aaaagatcag
     2401 ggccatatag aggataaaca tcatggcgta gctgattccc caggtggcga aaccgctctc
     2461 ctccttggtg ccggcctcgc tcaccttgaa ggtcttgaga tccattctct gcgtcagctt
     2521 gcgcaccagt tcctggtcga ggccgctctc ggccagccgc aggtcggaga ccacccgtga
     2581 gagcgaacgc tccagggcgg cattgaactc catgttgctg atgttgcggg cgtggagttc
     2641 gaactggctg gtcagaaaga tatcggccgg caggatgatc agcgcactca gtttttcctg
     2701 ctgcacgagc aggctgtaac gggccgtcac tgcagccagg ctctccggga cggtggtgac
     2761 cgtctcaaag gtgtaaagcg gcaccttgcg gtcgtcccgg aaattttcat ccatgtaggc
     2821 tgtgtaggcc ggaccgactt cgcccgtgag gtcgatgagg gcgagcgtct ttttctcacg
     2881 ggcacccatg gactgaactg cgatctggat gcccatgatg acgacgatca gtacgggcag
     2941 agcaaaggtg ccgatgatga atcccttgtt cttgacccgg atgagaaatt cacgtttgat
     3001 cagaacccag gtcttattca tgatcgcctc cggcgttagc gcctcctttg accacggtga
     3061 taaagatctc gttcagagag ggctcggtga tgttaaagct gcgtaccgta acctggctga
     3121 cagcctgggc cagaatcgcc tgcggctgga ccccctcggc gggctggatt tcgacatact
     3181 ggccataatc gtcatacctg ctcaccagcg cggtatcctt gaggaagcgg gcctccccgt
     3241 catagctgag gcggcagcgg ttgcggccga aacggccctt gacctcggcc aggcggccct
     3301 ggaggacgct gcgccccttg tcgatcagac agagagcgtc gcacagcttc tccgcctgct
     3361 ccatcagatg ggtggagaag atgatggtgg tgccgttttt gaccagatcg agcatgacat
     3421 ccttgatgag gttggtgttg accggatcga gtccgctgaa gggctcatca aggaagatca
     3481 atctgggctc atgcagaatg gtggtgatga actgcacctt ttgctgcatg cccttggaga
     3541 gttcctcgac ctttttatcc cgccaaggct tgagttcaaa acgctccagc cagatttcct
     3601 ccagtggagt ggctttccgt ggggggatac ccttgagctc agcgaagaac cggatgatgt
     3661 caatgatcac catacgcttg aagagtccgc gttcttcggg gagaaatccc acctgattgg
     3721 ccacctcccg gcagcttgat ttgccgagga tttcgatcgt ccccgagtcc ggcatgagaa
     3781 tgccgagcag catgcggatg gtggtggtct tgccggctcc attaggtccg atcaggccaa
     3841 agatctctcc ctggggtatc tccagggaga gattgtccac cgcccgaacg gtgtcgaaac
     3901 tttttatgac cttgttgagt gttaccgcac ttctcacggt tgcctcctga aagagtgtta
     3961 tgcaatggca tccgacgact atataaatat acgttaaaat cagattcgaa aaaagcattt
     4021 ctgtatacgc agccataaga gaggggtttc ccccaatcag gacgcggccg gggggctctc
     4081 gtttttctgt tcctcccgat gaatgtcctt gcgcggtaaa tatattgatt tttaattgca
     4141 tatttgctaa cttgctgcgt taattggacg attcacccct tctcaggagc atgaaatgcg
     4201 ttggcaagga agaactgaaa gtgaaaatgt tgaagatcgc cgcggcacaa cctcacgcag
     4261 agtgattggc ggcggcatcg gcaccctggt catccttctg ctggtctggc tgctgggcgg
     4321 cgaccccatg cagtttttgc aggagaatcc cggagtctcg acctccacac cggtctccac
     4381 agaggagcgt tccgcggagg aaaatcagct ggcctccttt gtcaaggtgg ttctgaaaga
     4441 taccgaggat gtctggggca agcttttcca gaatgcggga tcgcagtacc ggatgcccac
     4501 cctggtactc tacactgatc aggtccagtc cgcctgtggc tacggctcgg ctgcgacagg
     4561 gcccttttac tgtccgggag atgagaaggt ctacatcgat ctcgccttct gtgcggagct
     4621 ccagaaccag tacgatgctc ccggtgattt tgccatcgct tacgttatcg cccatgaggt
     4681 cggccatcat gtccagaacc tgctcggcat cactgaccag atcgccagca tgcaggggcg
     4741 cgtcagccag gaagagtaca accagtactc catccgcctc gagctgcagg ccgacttttt
     4801 cgccggcgtc tgggcccatc atgcccagaa gatgaacaac atcctcgagc ctggggacct
     4861 tgatgaggcg atcaacgccg cttccgcggt cggtgatgac cgcatcatgc gccgcaccca
     4921 gggctatgtg gtccctgatg cctttacgca tggcacctcg gatcagcgcc aacgctggtt
     4981 caagaagggc tgggataccg gtgatcccaa ccagggcgat accttcgcgg ccagaacact
     5041 ctgatcgacc cctttacgat agctggaacc ggctggcggc cggacggcag ggggctcaga
     5101 atacctcatc ccgggcggat gagcggattg taaccatcag gagcatgcaa atgaggttac
     5161 tgaaaggcgg attcctgttt atggcggcga ttctctatgc agcagcagcc gcagccctcg
     5221 agctgccggc gattttcggg gaccatatgg tgctgcagca gagacagcgc gtcgctttct
     5281 ggggaaaggc agcgccagga gagaaggttg gcctcgaggc ctcctggggc gcccgcagtg
     5341 ctgcgattgc ggacaacaac ggccagtggc gagccttcct caagactcca gcggcgggag
     5401 gaccctggac ggtcaccgtg accagcccct ccggcaccaa aagtttcatg gatgtcctca
     5461 ccggcgaggt ctggatttgc tccggccagt ccaacatgga gatgccgctt gccggctggc
     5521 cgccgggcga cccgatcgcc aactccgcgg ctgaaattgc cgccgccgat ttcccgcaga
     5581 tgcgcctgtt cacggtcgag agggccattg ccgccgcccc tgttgatgac tgtatcggcc
     5641 gctggcagcc ctgcactccg cagactgcgg ccgctttcag tgctacggcc ttctttttcg
     5701 gccgcaccct gctcaacgag ctcaatatcc ccatcggcct catccatacc agctggggcg
     5761 gcaccccggc cgaagcctgg accagcgctg aatacctcaa gaccctgccc cggtatgcca
     5821 gtgtactcga aaaggtcaag accagcagca cgcagcttga agcccgcaaa cgctggctgc
     5881 agaaccatgc cgtctttccc gtcctcaaga gcggcagcga ccgctgggtc aatctggatt
     5941 tcggtgacag catgtgtcct gcctacagct atgacgactc gcgctggggc cgcatgcagc
     6001 tgccgggctc gtgggaggcc accgaggtcg gccagtttga cggcgcggtc tggttccgca
     6061 agctggttga aatcccggcg gagtgggcgg gggaagaact cctgctcgaa ctcggcccca
     6121 tcgatgacat ggatgtcacc tatttcaatg gcgagcgcgt tgggggtatg gaacaggacg
     6181 gtctctggca gaccgaacgc ctctatacgg tgccggcaga gctggtcagg agcggcgcca
     6241 acctggtggc ggtgcgcgtt ctcgataccc aggggggagg cggcatctat ggccgcaagg
     6301 aacagctccg cctctatccc cgcagcaaac ccgatcgggc gatttccctg gccggcgagt
     6361 ggcgctacct gcccgtagcg gaataccaga acgcccgctt ttaccagttc gacgtcgcag
     6421 cagccgattt cttcgaccgc ccgaaggtag aggtggagat ctccgccaac accccctcgt
     6481 ttctttacaa tgccatgatc gcccccctgg tgccctatac cctgcgcggg gccatctggt
     6541 atcagggcga atcgaattcc ggccggccgc tggagtacag gaccctcttt ccgctcatga
     6601 tcgataactg gcgccgggcc tggggcggaa cccccttccc cttttattac gtacagatcg
     6661 cgccctatga ttacggcgca gccacccctt ccgaggagct gcgcgaggcg cagatgctca
     6721 ccctcagcca tcccaaaaca ggcatggcgg tgacgatgga tatcgccgct ccaagcatcc
     6781 atccacccaa caagcaggag gtgggcagac ggctggcgct ctgggccctg gaccgggatt
     6841 acggacacaa gcaggtccat tccgggccca tctatagaaa aatgaaaatc aagggcgagc
     6901 gcatcgaact cacctttgat cacagcggca gcggattgaa ggcggccgcg gaggggctca
     6961 ccggttttga aatcgctgga gccgacaaaa agttcgtccc cgccgcagct gtgattgatg
     7021 gccgcagggt ggtggtctcg agcaccgcgg tgcccaaacc cgtcgctgta cgctacgcct
     7081 ggagcaatct cgcccgggca accctcttca atctcgaggg gctgccggct tcctcttttc
     7141 gcaccgacaa ctggcaatta tcgattaact aattcaataa ggaagagtac catgccgaag
     7201 agctatttcc ccgagatcaa aggccccatc cccttcgagg gcaagggttc gaaaaatccc
     7261 ctcgcattca ggtactacaa caaggatcag aaggtcggcg gcaagaccat ggccgaacat
     7321 ctgcgttttg cggtggccta ttggcatacc atgatggcgg atggcaccga catgttcggc
     7381 ggagcgagct atgaccgcgc ctggcatgcc cccagcgatc ccatcgaccg cgccaaggcg
     7441 accatggacg ccgcctttga attcttcacc aaactgggcg ttgattatta ctgctttcat
     7501 gaccgcgaca tcgccccggc gggcgaatcc tttgccggat catgtaaaaa cctcgaaatc
     7561 atggtcgatt acgccaaggg cctgcaaaag cagaccggcg tgaagctcct ctggggcacc
     7621 gccaacctct tcggccatcg catcttcacc gccggcgcct ccaccaatcc ggatgcccat
     7681 gtcttcgccc tggccgccgc ccaggtgaaa aatgccatgg atgccaccct cgccctcggc
     7741 ggcgaaaatt atgtcttctg gggcgggcgc gagggttacg agtccctgct caataccgat
     7801 ctgaagcacg agcaggagca gctggcccgc tttttccata tggcggtcga ttacgccaaa
     7861 tccatcggct tcaagggcca gttcctcatc gagcccaaac cctgcgagcc gaccaaacac
     7921 cagtatgaca cggataccgc cactgccctg gctttcctgc gccagtttga tctcatcgac
     7981 tatttcaaga tcaatgtcga ggccaatcac gccaccctcg ccaaacacac cttcgagcac
     8041 gagctcacag tcgcctccgc cgcgggcaaa ctgggcagcg tcgatgccaa tcgcggcgac
     8101 ctcctcctcg gctgggacac cgatcagttt ccgaccgata tctacagcac caccctggcg
     8161 atgctggtca tcctcaagca gaacggcctc ggcagcggcg gtctaaattt cgatgcccat
     8221 gtacgccgca gctcgatcga tccggtcgac cttttccatg cccatatcgg cggcatggat
     8281 gccttcgccc gcggcttgct cgtcgcgcac aggattatcg aggataaggc cctgagctcc
     8341 ttcatcgatg aacgctatgt gagctacaag tcggggatcg gcgcaaaaat catggccgga
     8401 caggtcggtt ttgccgagct ggagaagtat atcctcgaaa aggggatgcc ggtgatgagg
     8461 agcggccgcc tcgagatgct cgagaatctc ctcaacgact atatctgatg ccgctttaaa
     8521 aaaagcgccg gtccgaaggg aacggcgctt ttttttttgc caagactgaa actaaggctc
     8581 acttttctgt gctgaagggc gaagccggca gcccctcccg gttgatgaga ttggcgccgg
     8641 cgggattgtc cgcccagccg tagcgcacca ctgccggacg ggatatcgct tcgctccaga
     8701 cctcgaccct gtcgccggtg atgcgggctt gagcccagac aaagcggccg tcggctccgg
     8761 cgatggcgaa atggcgcagc tcacctccgt cccggacggc caggccgctg ccggtatggt
     8821 caaacgagag gatgattttg ctgccctcga cccggctggt gcggaggaga gggccggatg
     8881 gaaccaactc tttatcctta taggccagcc gcctcgccca cagagccagc ctctcaccca
     8941 cattccgctt gttgagggga tgaatgtcat tccattcgcc gatgtcgatg gccaccgcca
     9001 tgccggtgtt ggggagggcg agggtcttgc gctgcgcctc gcgcagggcc gcccagccgc
     9061 tctctgcggg ctcggtgcgg gccaccatga aattggccag ctgcacatag aggaagggaa
     9121 acttgccctg gctgaactgc tgccgccaat cccggatcag atccgggaac aggcgggtat
     9181 attcctgcgc ccggccggtg ttcgactcgc cctgatacca gatggccccc ttgatccggt
     9241 agttgagcag cggggcgatc atgccgttga agagtcccag cggcttgtac tggatgaagg
     9301 tctggcccgc caggggctcc ataactgctc cgacctggca gcgccattcg ccgctcagat
     9361 cgagggtgtc ccccgcgacg acgagctggt aggccttgtc cttgataaag ccgccgctgc
     9421 cgatgttgct gatcacccgc accgtcagga ggtttttacc ggctttaagg agtccggccg
     9481 ggaccgcgta cctgcgcggg ggatactggt atgaggtggt gccgacgaag gtaccgttga
     9541 gaaagaccga atcggcgtcc acgattgccc ccagaatcag gcgcccaggc tggccggcca
     9601 tcgtctccgg cagatcgatc tccctgcgga accagaccgc gccgttgatg ggtcccaggc
     9661 cacgctcctt ccagaaggag ggcaccggca tcattggcca ggatgagtcg tcataatccg
     9721 gatcggccca ggaggcggcg ggatcggcat agcccgcgtc ttgtgcgcgc agcagcctga
     9781 accagctccc gatgcgcgcc tgatccgctg cgaggatgcc ctgaacgtag ctttcatcgc
     9841 ggcagcggag ggcgctgtcc agatggacgg gccaggcttg cagggccttc tcgctcatcc
     9901 aggcttcgac cggtgtgccg ccgacgctgg cgttgatcaa tccgatgggc acgccgtatt
     9961 tttcataaag cgccagggcg aagaagtagg ctgcggctga aaatccgcgc acagtctccg
    10021 cagtcgcccc ctgccaggcg cccgcctcca gatcctcctg cggacgattg aaatcatagc
    10081 gcatgggcac aaaaaagtgg cggatggcgg gattgtcagc cccggcgacg agatcggggt
    10141 agcgctcctc cacccgggcg atgggcaggg ccatattcga ctggcccgag cagagccaga
    10201 cctctccggc atagatatcc ttaagggtga tgcggtcctc accctcgatg cccatcgtga
    10261 aggggccgcc cgccgcgccg ctcctgatgc gcagaagcca gcgtccctcg ccgtcggccg
    10321 cagtgcgata atgtctgccc ctgaactcga ggttgacctt ttcgcccggg cgggcccagc
    10381 cccagatatt gacccggctc tcccgctgca gaaccatgcc gtcgctgatc aaacgcggca
    10441 gcctcacttc ggcgaatcca cttcccgcgg cgaagatcag cagcagccag actgcagctg
    10501 gtatcagctt gccgaacggt ttcatctctc ctctccctgc ttgatccatc agcgataact
    10561 atacagtttc accctgagca tggtataatc ctgcccaggc agttggatat ggcgtccctg
    10621 atgggtctgg tcgccgttga ggcggcgtcc ggagtgccag acgccttcaa tcatacggcc
    10681 ctcctcgacg ctgccgatgc ccgccctgac accttcggcc ctgggcgcga cctcgatgat
    10741 cagtccggta ccggcgacga tgaattcgtc gtcggccagg cggaggaaga gcccgccgta
    10801 gcgcggcatc accccctcct cgcgccgggc gtagctccag ctgtattcgt gacgaatgcg
    10861 caggagataa tcccccatct cgatccgggc gtgctgcccg gtgctgtcca gcagcacccc
    10921 gcgcatgctc cccttgccct gatgggtgat gagtaggggc tggagctgac gcagcagcgc
    10981 ataagcacgc gagaggggat tgcgaccggg atcggccaga tcctcgacgc tgaaggggct
    11041 atagcccatg gcgtcgtgct cgccgatggc gtaaaaggca ttggccggac tctggtcgtt
    11101 gcccacctcc ggaatgaaga ggggatttcc gggcacgtcg tactggacgg cccattcccg
    11161 gaaattcctg aaatagatat cgggagcgag aaaatcgatc tgcggcgccc ccgcccgcca
    11221 gatgtcaatg agatggggga gaggaccggc gctgggatac tggccgggct gatatcccgg
    11281 acggatcagg gcggcgttga cgtacatcgg cagcgggtac tccgccttcc ctgcccgcgc
    11341 cacctcatcg gtataacgcg cgaagtgcca ggccatgaag aactcgtcgg tggccgggcc
    11401 gatgccgaaa agggtttccc agtttcccct gaccgggctg ccctgctttt cccaggcctc
    11461 cctgagcctg gcatgcagcg tttttttgtt cttctgcata taggcgacga gatcggccgg
    11521 caccgcctcg gcaaagcgtt tgtccgcctc gcggcagtga tcgcgtgcat ccgggatcat
    11581 gccgatctcg ttctccacct ggaccatgac caccgtctgc tccttctcat ccattttgcg
    11641 gagatggcgc atcagggcgg tgaaggcgcg tacatcggct tggagattct ccgcgctgaa
    11701 cggagtgagg atttcctggg gctctcctgc gcgcgtgcgg gcgcggggga agcgcaccgg
    11761 gtccgatttc acccagaggg gcgcgtagca ggacatgctg ttcttccagg tgccgaacca
    11821 gagcaggacg agcttgaggt cgttggcgcg ggcggcctgg aggatgctgt cgacgagcgc
    11881 aaattcaaaa cgcccctcct ccggctcgat cagctcccag tagatgggca ccagggctgt
    11941 gttgagatgc atgttcttca gcttgggcca gatccagcgc atcgatgcgg catcggaggc
    12001 gctcgaattg cccaattcac cggccagcat gagcaagggc ttgcccccga cgatgagctg
    12061 gaccgcctcg ccctgctggc ggagatgggg gatggccaaa gccccgtctt tcgggactga
    12121 ttcaccccga ctgcagagca gggtgagaag gatgagggaa aaggcggatt tgcgcatgga
    12181 taatctcaca gtatggagtt attttcaggc agacgccccg gtgcaggctg gtccagcctc
    12241 cgcccactga cctcactggt gccggcaaag cgggcgggaa gagaaacacc ctctatttga
    12301 ggatgatcat cctgcgtgtg gccgtgaatc cggctgtgcg cagggtgtag agataggtgc
    12361 ccgacttgag gattgtggca tcaaaccgga aactgtgcgt gccggcctcc agggtgcggc
    12421 tgaccaggat cgccatctcg cggccgagca ggttgtgaac cttgagttcg acatggccgg
    12481 cggctgccag ggtgaagcgg atggtcgtcg ccggattgaa ggggttggga taattctgcg
    12541 caagctcgaa aagctcaggc gaccgggctg gagcctcctc aaccgaactc tgcaccccgg
    12601 tttcaaacca ggcgggggcg gagaactcgc ttgacccata ggtgttgagg gcactgacgc
    12661 gccagtaaaa gcggctgttg gcggccagct tgccgagctg gaaggtggta tcagccacga
    12721 tggtgtccat ggcgatgctg gtgaaggccc gggccgttga aagctgcacc cggtatgaat
    12781 cagcgagagc ggtggaatgc cagagcagca gcgggttgcg cagctcgccc agggtgccgt
    12841 tggggccgac caggagaggg gagatgggct ttttgatata gtcgcgcagc cagggcatga
    12901 ccggacgttc ggtcccgttg gccttgagca gataggtata gggatgggag gtccagacgt
    12961 cgccctcgat gtagccccag aaggtgatcc ccttgaccgc aggctggttc cagaagaggg
    13021 ggaagtagat ctggtattga gcgagctgca cagcgtcgtc gggctcgtcg atgtcgaatt
    13081 cggtgatgta gataggcaga ccgacggccg cgatgcgctc gagattcgcc ttgagggtgg
    13141 tgatgctgta gacatactgg ttggcggagc gggtgtcgct gcggaactca aaatagtggc
    13201 cctggatgcc gattccgtcg accagtcc
//
LOCUS       DAOPWU010000062        13013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4887_length_13013_cov_5.260766, whole genome shotgun sequence.
ACCESSION   DAOPWU010000062 DAOPWU010000000
VERSION     DAOPWU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4887_length_13013_cov_5.260766"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1167
                     /locus_tag="PKM23_05085"
     CDS             <1..>1167
                     /locus_tag="PKM23_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90881.1"
                     /translation="DSSEVVTLHLGAVAVPRINGSYGGETDRYDPATGLAYCFRGDEA
                     AYAGLVLLSSTPYSYKALDWNTYSPADPNSDAATDSTRYHQTADPGFGPEVVAGGDGS
                     IYSLNAGPVTLAPHDSVVVVYGYVFGASFNEMAAAAEAAQTAYSLKIAENPASTYFES
                     GTIGVRVSGAGSIRLLTPNTSGIWQLARVNIIAALNESAVCDYNENHNPLDGTVMLAH
                     PTKADIEGFVHFDSRYYDEDDPASTPLPPDMSFDLHTYMWTDQPYVIGEYTVTSDMTE
                     VLTFHIGAVSVPSVGDNYGGETVKYHAGTRMVYCYREGESDFSGVCLLSHEPYSYKVL
                     DFADYSPEDPASDAATDSTRYHMTADAGFDADLIASGDGSIFSLNAGAFTLAPQA"
     assembly_gap    1168..1267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1268..3653
                     /locus_tag="PKM23_05090"
     CDS             <1268..3653
                     /locus_tag="PKM23_05090"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY90882.1"
                     /translation="ITDAQTGEPLPGANIQIVGSTIGAVSDMDGRYIILNVPPGTVSV
                     KVTFIGYESQIVTNVRIMIDLTTTVSAKLVPSSVQMKEVVIIADKPMIQKDVTSSGAT
                     MRREEIESLPVSEFTDVLALQAGVTDMGGTLHLRGGRSNEVGFMIDGMYVQDPLLGRM
                     ITQINNDAIQELNLLSGTYNAEYGNALSGIVNIVTRDGGEVQSGNIEFRSSQFGIARY
                     NRLEENRLNGSLGGPLLLKHLKYFISGEQNNKGSYLPFGYDRSRSLFGKLTYQLKPTV
                     KFTLSNRNTRSRYQRYSHSYKYIPEQYLRHRSDSHQTLFMVNHSIKPNLFYDMRVSFL
                     DEKYYSGIDKDTSAYLSATEAEYIPWAGDGYEFYARADYPELFDSHNKTADFKTDMVW
                     QLNKINEIKMGVQYRKHWLKLFYVYDPKRNYPYLNDYNIKPFEASGYIQDKIEFPFLI
                     INLGLRYDYFNANATFRKNPLSQDDLITVKARTQLSPRLGIAHPVSERTKIHFAYGHF
                     FQNPEYQYLFENRQYDLNVREPLFGQPDLDAQQTVSYEVGVSHQFSERIAAHLTAHYK
                     DITDYIGTRYYEAFSGNTGRFVGYTVYVNEDYANDKGFEVNVEMRPGRYFGGGLSYTF
                     SIAKGSASSEAEQYPGTDESTKLYYLDWDQRHNLSMQALFRVPREEGPRLLGKRLLAD
                     TDYSLVLRMASGFPYTPSGRDIGFVDRNSLRRPGTYTIDLEAGKRFTLGRGFSLRAFV
                     ECLNITDHRNIRWVYGDTGDPDFTLVGGYSTEYMRDPSNYGPPRSIRLGLGLQR"
     gene            3679..6486
                     /locus_tag="PKM23_05095"
     CDS             3679..6486
                     /locus_tag="PKM23_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90883.1"
                     /translation="MMKKIALWAGLVCLITGAALAGPGGSDKDRARNDESALRKSLGV
                     EDRAGGTHNASNIGLFFENRGKLYPRRLTQGPSGEYPIGSGRNYIYRINPMVGVPGNV
                     IQGRYTTNEEWEAVGGYHNRSYAKIAFSDNPLSWDPVLGWPVRDKAGNPVVKSDQDSY
                     CVYDDHNNTITPLGVQVMQTGYAYGVKFAQNLLFFKFEITNKGEKDLDSLYFSMYCDI
                     DVGDISGGAPEYGDDKLEFDRANNMVYFFDDGASSEWSGNVTGYFGIALLESPPIGGL
                     EAGITDFHYNLYDDDRDDDSLQFAIMSSNPGLIRPQSLVPFYFHQGANPDRHFDDPAT
                     IPASGLDLLANFSSGPHRLERGDTLVFYTACIAGTNKEDLYTSLKTAQKILAFNFEIS
                     KPPTTPILSAHAGDRQVTLFWDNAAERSLDTYSGEYDFEGYRIYRSVDKGLHWDQLDR
                     NVDPSVGIDPVPIAKFDLVNNIGLDLGLQHSFTDTTVENGFEYWYTLTAYDRGDASVE
                     SLESPKGNTPDALNTVAVQPVSSAANRTPVAAGEVGHSRGKSTYVLDVQPVDQDSLGD
                     RSYTVRFDYQQQTTRGTLRTRIIPVITDSSLTPGTHIGIEFAAPNSFHLIDLNTGDYI
                     GSDPRSYRSGTTYSINKGMRIKVEDPDPAAAPQYLPKAGDYLTLFYALEVTRNDGQVV
                     TPLQPVFYEKPYATRDGVIFTLKQPQYIQSVSRIGGTDNLDITFEVADESAVKAASYQ
                     ISTTAGGKDSAGKGFVSLLISTPGGELTVDSLYTGDSFDFDGLSGTLSFPAAQPPQPG
                     NIFAVETVLPILPSLQDRYTFRIEGAKVTAARVQNQLDRIRVVPNPYLVASLYEPEFG
                     ELRKEPLRQIQFINLPQKCTIYIYTVAGDLVKTIEHEAAHGTEQWDLRADGGREIAPG
                     IYLYIVKTDGAQFKNTFAVIK"
     gene            6496..7494
                     /locus_tag="PKM23_05100"
     CDS             6496..7494
                     /locus_tag="PKM23_05100"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY90884.1"
                     /translation="MNTISRWILIALLALSALAQAQNPNLGTSGAQFLKIPVSARAAA
                     MGGAYVAVCQDATSPFWNPAGIAGIKGHAAHFSHTRWFDTFDVSAASYVYNAGSFGAF
                     AASVMMLGVDEMEITTEFQPNGTGAYFDAQDLAVGLSYGRELTDRFRVGLTARYIQQR
                     IWNESAGGLAFDVGTQYQLPFRNLVIAMSMSNFGADMRYNGSDMSVKWDGDANFPNRL
                     VPTRLETEAFALPLNFAFGIAMDLFRAPYARGLVALDAVHPNDNKECIHLGTEITFFD
                     RLALRGGWKVNGEEELYDLGFGVNAFSLGIPLSFDYSYSAYDILPDVHRFSFGLSF"
     gene            complement(7588..9276)
                     /locus_tag="PKM23_05105"
     CDS             complement(7588..9276)
                     /locus_tag="PKM23_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HNY90885.1"
                     /translation="MLRRMLICSLLLFACLLPAGAQSLLTPGEINGYSRYTQHEEVVR
                     FLSALSAHSRTLQVREVGRTRPVEGFSAKSLLLCILTAEGVNRPEQLDRSKPTLFITA
                     AKHGSEQSAKEAALLILRDFAVGELKSLLEKVNLLIMPMVNPWGNYFDRRVNEIGLDM
                     NRDHIKMESEGVQAIHRVFRAWMPEVTLDVHEQGDKYYRISMGCVSNLNVDPAIEAYS
                     RRVILNRVGEALARDKITFHEYIVTSENMPSDASGADFPDEELARWPQITRHSTTDIN
                     DGRNILGLYQTFSFIQEAASRHDLATLAARTRWQERGIRAFIQAVAEHGPEMTAQVNG
                     LRRQLLEQATVLNPEHRVHIRMEYRRDPARPDLRLKEFQRREPAAAGRLKVEKRAGEV
                     LLRSEIEPLPELDGQKVVERVEKEWYPLVAPTLSVVRPLSYVIPAQHAEVIATLQLHG
                     IAINVFTEEVPVKVEGSFITAVTPSKYDYVAPERIEVEARRLVLPCKKGDFYISCRQP
                     AANLLPCLLEPASDFGLVRYWKYGLVPEATTFYPFYRVIEEQAPPLVPYAAWPD"
     gene            complement(9281..10417)
                     /gene="menC"
                     /locus_tag="PKM23_05110"
     CDS             complement(9281..10417)
                     /gene="menC"
                     /locus_tag="PKM23_05110"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HNY90886.1"
                     /translation="MKFLQTPIGPVDHIDLLTVRLPFIQPFAISSYAWSCKEALLLRV
                     EAEGLVGWGECVADPDPFYAPETTGSCRYIIEKFLLPLLEPGMTLGDLSERMHKLRGN
                     QMARATFENAVIDLLAKQQGLRLYEFLGLPEKKIMSGISLGLQESPDKLVQAVEEAIA
                     KGYHRVKMKVKQGQDVEWVAAVRQRFPDIPLMVDANGDYTLADGSRLAGLDAFGLMMI
                     EQPLSYSDIFQHSLLQRRLQTPLCLDESIHSLEDAETALALGACRIINIKQGRVGGLI
                     ESLRMAELAAAAGVPVWSGGMDETGIGRAVNIHLQTAAGFTLPGDTSETSRYFHEDIA
                     DPPVVLDGEGYIAIPPGPGIGVEVPADRLARYTIQQERLYSRKG"
     gene            complement(10518..11660)
                     /locus_tag="PKM23_05115"
     CDS             complement(10518..11660)
                     /locus_tag="PKM23_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNY90887.1"
                     /translation="MRLGRIFSLVLFFCALLSACGKKESPTGPKDEKPVVTNTILVDS
                     LATPETVALFNNLKSLAQTNLLFGHQDDLAYGVGWWAAPGRSDVKEVCGDYPAVYGWD
                     LGDIHKQTNLDGVYFDAMKQWIRQVYERGGINTISMHLDNPYTGGSAWDNSAAVKYIL
                     PGQSHHAAYLKTLDQIAAFLKDLKSSSGTAIPIVLRPYHEHNHNWPWWGAGACTVPEY
                     NALWKMTVGYLRDTRGVHNLLYAISPQEVNTIDQYLARYPGDEWVDILGMDYYLLTSK
                     GRIDDLGKSLAMMAELAEARGKISALTEVGWQNVPVSDYWTGYLLPAVMYNAQSKKTT
                     WALVWRNASESQYWAPYVGQISATDFSLFHLHPFTFFEKDLPAMYK"
     gene            11763..12995
                     /locus_tag="PKM23_05120"
     CDS             11763..12995
                     /locus_tag="PKM23_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY90888.1"
                     /translation="MNILYISQYFPPEVGATQNRAFEMAANLVRLGHKVTILTEFPNH
                     PGGIIPPEYRRRWYVKDELAGIRVVRVWVFARPVKTFFTRMGFYLTFMVNAALVGTLV
                     RGPYDIVYATSPPYFVGVTALWLSLVKRARFVFEVRDLWVQSAAELGELSNPRLLKMA
                     GWLDHLYYRKAWRIIAVTRGIYDALATRGLAAKLHLIYNGTNCDLLYDRGSGKRLELG
                     WEKKFVVMYAGILGIAQGLEEICRVIAQLKSDPEIQFAFVGAGPLRQRIETLQKEQGL
                     TNLTLLGEIPRERIADYLSAADCCLVPLKRRETFRGALPSKMFDVMACSRPLLLSVDG
                     EARELVEKIGAGLYVEPENDDELRQAILALKNDPALGKKMGAAGRAFVENNFSRRQAA
                     IELERVLQDVLNESRRSA"
BASE COUNT         2540 a         4322 c         3633 g         2418 t
ORIGIN      
        1 cgactcctcc gaggtggtca ccctccatct cggggctgtg gcagtgccgc gcatcaatgg
       61 cagctacggg ggcgagaccg accgctatga tccggccacc gggctggcct actgcttccg
      121 cggcgatgaa gccgcctacg ccggcctcgt tctgctctct agtactccct attcctacaa
      181 ggccctcgac tggaacacct attcgccggc cgatcccaac agcgatgcgg cgaccgattc
      241 gacccgttac caccagaccg ccgatccggg cttcggtccc gaagtcgtgg ccgggggcga
      301 tggctcgatc tacagcctca acgccggccc cgtcaccctg gctcctcatg attcggtggt
      361 agtcgtttac gggtacgtct tcggcgctag ttttaacgag atggccgccg ccgctgaggc
      421 cgcccaaacc gcctactcgc tcaagatcgc cgagaatccc gcgagcacct atttcgagtc
      481 cggcaccatc ggcgtccgcg tgagcggtgc cggcagcatc cgcctgctca cccccaacac
      541 ctccggcatc tggcagctgg cccgagtcaa catcatcgca gcgctcaacg agagcgcggt
      601 ctgcgactat aacgaaaatc ataatcctct cgatggcacc gtgatgctcg cccacccgac
      661 caaagcggat atcgagggct ttgtccattt cgacagccgc tactacgatg aggatgatcc
      721 ggcctccacg cccctgccgc ccgacatgag cttcgatctc cacacctaca tgtggacgga
      781 tcagccctac gtcatcggcg aatacacggt caccagcgat atgaccgagg tgctcacctt
      841 ccatatcggc gcggtctcgg tgccgagcgt gggcgacaac tatggcgggg aaaccgtcaa
      901 gtaccacgcc ggcacccgta tggtctattg ctatcgcgag ggcgagtccg atttctccgg
      961 cgtctgcctg ctctcccacg agccttattc ctacaaggtc ctggattttg ccgactattc
     1021 acccgaggat cccgccagcg atgcggccac cgattccacc cgctaccata tgactgcgga
     1081 tgccggcttt gatgcggatc tgatcgccag cggcgacggt tccatcttca gcctcaacgc
     1141 cggggccttc accctcgccc cgcaggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnncat cacggacgcc cagaccggag aacccctgcc cggcgccaac atccagatcg
     1321 tcggcagcac catcggcgcc gtctccgata tggatggccg gtacatcatc ctcaacgtcc
     1381 cgcccggcac tgtatcggtc aaggtgactt ttatcggtta tgagagccag atcgtcacca
     1441 atgtacgcat tatgatcgac ctgaccacca cggtatcggc caaactcgtt ccctcctcgg
     1501 tgcagatgaa agaggtggtc atcatcgccg acaagccgat gatccagaaa gacgtcacca
     1561 gttccggggc gacgatgcgg cgggaggaga ttgaaagcct gccggtctcc gagttcaccg
     1621 atgtgctcgc cctccaggcc ggcgtcaccg acatgggcgg aacgcttcat ctgcgcggcg
     1681 gccgctccaa tgaggtcggc tttatgatcg acggcatgta tgtgcaggac cccctcctcg
     1741 gccgcatgat cacccagatc aataacgacg ccatccagga actcaacctg ctcagcggca
     1801 cctacaatgc ggaatacggc aacgccctca gcggcatcgt caacatcgtc acccgcgacg
     1861 gcggcgaggt ccagtccgga aatatcgagt tccgctccag ccagttcggc atcgcacgct
     1921 acaaccggct cgaggaaaac cgcctcaacg gcagcctcgg cggtcccctc ctcctcaagc
     1981 acctcaagta tttcatctcg ggtgaacaga acaacaaggg cagctacctg cccttcggct
     2041 acgaccgcag ccgcagcctc ttcggcaagc tcacttacca gctcaagccg acggtgaaat
     2101 tcaccctcag caaccgcaac acccggtcca gatatcagcg ttacagccac agctacaagt
     2161 acatcccgga gcagtatctc cgccaccgct ccgactccca ccagaccctg ttcatggtca
     2221 accacagcat caagcccaac cttttttatg acatgcgcgt ctccttcctc gatgaaaagt
     2281 actactccgg catcgacaag gatacctccg cctatctcag cgccaccgag gcggagtata
     2341 ttccctgggc cggggacggc tatgaattct atgccagggc cgattatccc gaacttttcg
     2401 acagtcacaa caagaccgcc gatttcaaga ccgacatggt ctggcagctc aacaagatca
     2461 acgagattaa aatgggggtg cagtaccgca aacactggct caaactcttt tacgtttacg
     2521 acccgaagcg caactacccc tacctcaacg actacaacat caagcccttc gaggcctccg
     2581 gctacatcca ggacaagatt gaattcccct ttctgatcat caacctgggg ctgcgctacg
     2641 actattttaa cgccaatgcc acctttcgca agaatccgct ctcgcaggat gacctgatca
     2701 cggtcaaggc ccgcacccag ctgagcccgc ggctcggcat cgcccatccg gtctcggaac
     2761 ggaccaagat ccacttcgcc tacggccact ttttccagaa tccggagtat cagtacctct
     2821 ttgaaaaccg acagtatgac ctcaacgtgc gcgagcccct cttcggccag cccgacctcg
     2881 atgcccagca gaccgtcagc tatgaagtgg gggtgtcgca ccagttctcc gaacgcatcg
     2941 ccgcccacct caccgcccat tacaaggaca tcaccgatta catcggcacc cgctattacg
     3001 aagccttttc cggcaacacc ggccgcttcg tcggctatac cgtctatgtc aacgaggact
     3061 atgccaacga caagggcttt gaagtcaacg tcgagatgcg gccgggccgc tacttcggcg
     3121 gcggcctgag ctacaccttc tccatcgcca aggggagcgc ctcctccgag gccgagcagt
     3181 accccggtac cgatgagagc accaagctct attatctgga ctgggaccag cgccataacc
     3241 tcagcatgca ggccctcttc cgcgtgccca gggaggaggg gccccgtctc ttgggcaaac
     3301 gcctgctcgc cgataccgac tatagtttgg tgctgcgcat ggcctccgga tttccctata
     3361 ccccgagcgg acgcgacatc ggttttgtcg accgcaactc cctgcgccgg cccggcacct
     3421 acaccatcga cctggaggcc ggcaagaggt tcaccctcgg ccgcggcttc agcctgcgcg
     3481 ccttcgtcga atgcctgaac atcaccgacc accgcaacat ccgctgggtc tatggcgata
     3541 ccggcgatcc cgatttcacc ctcgtcggcg gctattcgac cgaatacatg cgcgatccct
     3601 ccaattacgg cccgccgcgc agcatccgcc tgggcctggg tctgcagcga taaacgaacc
     3661 ctcatgaacg gatcaacgat gatgaagaaa attgcactat gggccggcct ggtctgcctg
     3721 ataacgggcg cagcgctggc gggaccgggc ggcagcgaca aggaccgcgc gcgtaatgac
     3781 gaaagcgccc tgcgcaaatc cctcggggtc gaagaccggg cgggcggaac ccacaacgcc
     3841 agcaatatcg ggctcttttt cgagaaccgc ggcaagctct atccccgccg cctcacccag
     3901 ggcccctcgg gcgagtatcc catcggctcg gggcggaatt acatctatcg catcaatccc
     3961 atggtcggcg tccccggcaa tgtcatccag gggcgttata ccaccaatga ggagtgggaa
     4021 gcggtcggcg gctatcacaa ccgcagctac gccaagatcg ccttcagcga caatcccctc
     4081 tcctgggacc ccgttctcgg ctggccggtc agggacaagg cgggcaaccc ggtggtcaag
     4141 tcggatcagg acagctactg cgtctacgac gaccacaaca acaccatcac cccgctcggg
     4201 gtgcaggtga tgcagaccgg atacgcctac ggcgtcaaat tcgcccagaa cctcctcttt
     4261 ttcaagttcg agatcaccaa caagggggag aaggacctcg acagcctcta cttctccatg
     4321 tactgcgaca tcgatgtcgg cgacatcagc ggcggcgcgc cggaatacgg cgacgacaag
     4381 ctcgagttcg accgcgccaa caacatggtc tacttttttg acgacggggc ttcatcggag
     4441 tggtccggca acgtcaccgg ctacttcggc atcgccctgc tcgagagccc gcccatcggc
     4501 ggcctcgagg ccggcatcac cgatttccat tacaacctct atgacgacga ccgcgacgac
     4561 gacagcctgc agtttgccat catgtccagc aatcccggcc tgatccgccc gcagtcgctg
     4621 gtgcccttct acttccatca gggcgccaat ccggaccgcc acttcgacga ccccgccacc
     4681 atcccggcct ccggactcga cctgctcgcc aatttcagtt ccggtcccca ccgcctggaa
     4741 cgcggcgaca ctcttgtctt ctacaccgcc tgcatagccg gcaccaacaa ggaggacctc
     4801 tacacctccc taaagacagc ccaaaaaata ctcgccttca atttcgagat cagcaagccg
     4861 ccgaccactc ccattctcag cgcccacgcc ggggaccgcc aggtcaccct cttctgggac
     4921 aatgcggccg aacgctccct cgacacctat tccggcgaat acgactttga gggctaccgc
     4981 atctaccgca gtgtcgacaa ggggctgcat tgggatcagc tcgaccgcaa cgtcgatccg
     5041 agtgtgggca tcgatccggt tcccatcgcc aagttcgatc tggtcaacaa catcggcctc
     5101 gacctcggac tccagcacag ctttaccgat accacggtcg agaacggctt tgagtactgg
     5161 tacaccctca ccgcctatga ccgcggtgac gcctcggtcg aaagcctcga gagccccaag
     5221 ggcaacaccc ctgacgccct caataccgtc gcggtccagc cggtctccag cgcggccaac
     5281 cgcactcctg tcgccgccgg cgaggtcggg cacagccgcg gcaaatccac ctatgtgctc
     5341 gatgtccagc cggtcgacca ggactcgctc ggcgaccgct cctatacagt ccgctttgac
     5401 tatcagcagc agaccacccg cggcaccctg cgcacccgca tcatcccggt catcaccgat
     5461 tccagcctca cccccggcac ccacatcggc atcgaattcg ccgcgcccaa ctctttccat
     5521 ctcatcgatc tcaacaccgg cgattatatc ggctccgatc cgcgcagcta ccgatcgggc
     5581 accacctatt cgatcaacaa gggaatgcgc atcaaggtgg aggaccccga tccagccgcc
     5641 gcgccccagt acctgcccaa ggcgggggac tatctcaccc tcttttacgc cctcgaggtg
     5701 acgcgcaacg acggtcaggt ggtcacccca ctgcagccgg tgttttacga aaaaccttac
     5761 gccacccgcg acggcgtcat ctttaccctc aagcagcccc aatacattca gagcgtctcg
     5821 cgcatcggcg gcaccgacaa cctcgacatc acctttgagg tcgccgatga atccgcggtc
     5881 aaggctgcca gctaccagat ctcgaccacg gccggaggca aggacagcgc cggcaagggt
     5941 tttgtctcac tgctgatcag caccccgggt ggagagttga ccgttgactc cctctacacc
     6001 ggcgacagct ttgatttcga cggcctcagc ggcaccctct ccttccccgc cgcgcagccg
     6061 ccgcagccgg gcaatatctt cgccgtcgaa accgtgctgc ccatcctgcc ctccctgcag
     6121 gaccgctaca ccttccgcat cgagggcgcc aaagtgaccg cggcgcgggt gcagaaccag
     6181 ctcgaccgga tcagggtggt gcccaatccc tatctggtcg cctccctcta tgaacccgaa
     6241 ttcggcgaac tgcgcaagga gcccctgcgg cagatccagt tcatcaacct gccgcaaaaa
     6301 tgcaccatct acatctacac cgtggctggc gacctcgtca agaccatcga gcacgaagcg
     6361 gctcacggca ccgaacaatg ggacctccgc gccgacgggg gacgggaaat cgccccgggt
     6421 atctatctct atatcgtcaa gaccgacggg gcgcagttca agaacacctt tgcagtgatc
     6481 aagtaggagg gcggcatgaa cacgatcagc agatggatcc tcatagccct tctggcgttg
     6541 agcgcgctcg cacaagcgca aaatcccaac ctcggcacca gcggggccca gtttctaaaa
     6601 attccggtca gcgcgcgcgc cgcggccatg ggcggcgcct acgtggccgt ctgccaggac
     6661 gccacctcgc ccttctggaa tccggccggc atcgccggca tcaagggcca cgccgcccac
     6721 ttttcccaca cccgctggtt cgacaccttc gacgtcagcg ccgcctccta tgtctacaat
     6781 gccggcagct tcggcgcctt cgccgccagc gtcatgatgc tgggcgtcga cgagatggag
     6841 atcaccaccg aattccagcc caacggcacc ggcgcctatt tcgacgccca ggacctggcc
     6901 gtcggcctga gctacggcag ggagctgacc gaccgcttcc gcgtcggcct gaccgcgcgc
     6961 tacatccagc agcgcatctg gaatgaaagc gccggcggcc tcgccttcga cgtcggcacc
     7021 cagtaccagc tccccttccg caacctcgtc atcgccatga gcatgagcaa cttcggcgcc
     7081 gacatgcgct acaacggatc ggacatgagc gtcaaatggg acggcgacgc caacttcccc
     7141 aaccggctgg tcccgacccg gctcgagacc gaggcctttg ccctgcccct caatttcgcc
     7201 ttcggcatcg ccatggacct cttccgcgcg ccgtacgccc gggggcttgt cgccctcgat
     7261 gccgtgcacc ccaatgacaa caaggaatgc attcatctcg gcacggagat caccttcttc
     7321 gaccgccttg ccctgcgcgg cggctggaag gtcaacggag aagaggagct ctatgatctc
     7381 ggtttcggcg tcaacgcctt ctcgctcggc atccccctga gcttcgatta ttcctattcc
     7441 gcctacgaca tcctgcccga cgtgcaccgt ttttcgttcg gactctcctt ctgacccata
     7501 aaaaaagggc gggcttttca gcccgccctt tacggtctta ccactccctt atctcatact
     7561 ccaattttcg aggcctgtgc tgcgccctca gtccggccag gccgcatagg ggaccagggg
     7621 cggcgcctgt tcctcgatca ccctgtaaaa gggatagaaa gtggtggcct caggtaccag
     7681 gccgtatttc cagtagcgga ccaagccgaa atccgaggcc ggttcgagca gacagggcag
     7741 cagattggcg gcgggctggc ggcaggagat gtaaaagtcc ccttttttgc aggggaggac
     7801 gagacggcgc gcctcgacct cgatccgctc cggcgccaca tagtcgtatt tcgacggcgt
     7861 cacggcggta ataaagctgc cctcgacttt tacaggcacc tcctccgtga aaacattgat
     7921 cgcaatgcca tgaagctgaa gggtggcgat cacctccgca tgttgagccg ggatgacata
     7981 gctgagaggc cgtacgacgc tcagggtcgg cgccaccagg ggataccact ccttctcgac
     8041 gcgctcgacc accttctgcc cgtccagctc cggcaggggc tcgatttccg agcgcagcag
     8101 cacttcgccc gccctcttct ccaccttcaa cctgccggcc gccgccggct cccggcgctg
     8161 gaactctttc agccgcaggt cgggccgggc cggatcgcgg cggtactcca tcctgatatg
     8221 cacccgatgt tccggattta agaccgtagc ctgctcgagg agctgccggc gcaggccgtt
     8281 cacctgcgcg gtcatctccg gcccgtgctc cgccactgcc tgaatgaagg cgcggatgcc
     8341 ccgctcctgc cagcgcgtgc gcgcggccag ggtggccaga tcatgccgcg aagccgcctc
     8401 ctggatgaag gaaaaggttt gatagaggcc gagaatattg cggccgtcgt tgatgtccgt
     8461 ggtgctgtgg cgggtgatct gcggccagcg cgccagctcc tcgtcgggaa aatcggcgcc
     8521 ggaggcgtcg ctgggcatgt tctcggaggt gacaatatat tcgtgaaagg tgatcttgtc
     8581 ccgggcgagg gcctcaccga cacggttgag gatgacccgg cgggagtagg cctcgatggc
     8641 gggatcgacg ttgagattgg agacgcagcc catgctgatg cggtagtact tgtcgccctg
     8701 ttcatgcaca tcgagggtga cctcgggcat ccaggcgcgg aagacccggt ggatggcctg
     8761 cactccctcg ctctccattt tgatgtggtc gcggttcata tccaggccga tctcgtttac
     8821 ccggcggtca aagtaattgc cccagggatt gaccatgggc atgatcagaa ggtttacctt
     8881 ttcgagcagg gatttgagct cccccacggc gaaatcgcgg aggatgagca gggccgcctc
     8941 cttggccgac tgctcactgc cgtgtttggc tgcggtgatg aagagggtgg gcttgctgcg
     9001 gtcgagctgt tccggccggt tgaccccctc cgcggtgagg atgcagagca gcaggctttt
     9061 ggcggagaag ccctcgaccg gcctggtccg gccgacctca cggacctgca gcgtcctgga
     9121 gtgcgcggag agagcgctga gaaaccggac gacctcttca tgctgggtgt agcgggaata
     9181 gccgttgatc tcgcccgggg tgaggaggct ttgggcgccg gccggcagca agcatgcgaa
     9241 gagcaagaga gagcagatca gcatccgtcg cagcatggga tcatcctttc cttgagtaga
     9301 gccgttcctg ttggatggta tagcgcgcca gcctgtcggc cggcacctcc acgccgatgc
     9361 ccggtcccgg cggaatggcg atatagccct ccccgtcgag cacgaccggg ggatcggcga
     9421 tgtcctcgtg aaaatagcgg ctggtttccg aggtgtcgcc cggcagggtg aatccggcgg
     9481 cagtctgcag atgaatgttg accgcgcggc cgatgccggt ctcatccatt cctcccgacc
     9541 agaccggaac ccccgcggcg gcggccagct ccgccatgcg cagcgattcg atcagccccc
     9601 cgacccgccc ctgcttgatg ttgatgatcc ggcaggcccc gagggcgagg gcggtctcgg
     9661 catcctcgag gctgtggatc gattcatcga ggcagagagg ggtctgcagc cgccgctgca
     9721 gcagggaatg ctggaatata tcggaatagg agaggggctg ctcgatcatc atcagtccaa
     9781 aggcgtcgag cccggccaga cgggatccat ccgccagggt atagtcgccg ttggcatcca
     9841 ccatcaacgg gatatcgggg aagcgctggc gcaccgccgc cacccattcg acatcctgtc
     9901 cttgcttgac tttcatcttg acgcggtggt agcctttggc aatagcctct tccactgctt
     9961 gcacgagctt gtccgggctc tcctgcagcc ccaggctgat gcccgacatg atctttttct
    10021 cgggcaagcc gaggaactcg tacagccgca gcccctgctg tttggcgaga agatcgatga
    10081 ccgcgttttc gaaggtcgcc ctggccatct ggttgccgcg cagcttgtgc atccgctcgc
    10141 tcagatcgcc cagggtcatc ccgggctcga gcaggggcag gagaaatttc tcgatgatgt
    10201 atcggcagga gcctgtggtc tccggggcat aaaaaggatc ggggtcggcg acgcactcgc
    10261 cccagccgac gagtccctcc gcctccaccc gcagaaggag cgcctccttg caggaccagg
    10321 cgtaggagct gatggcaaag ggttgaatga aggggagccg gaccgtcaac aggtcgatgt
    10381 ggtcgacagg tccgatgggg gtctggagaa atttcatttg gcgcctcgta ttccggttta
    10441 ccgtccgcct cacttataca ttcccggcag atcctttacg tcgcccccgg ccgctctcgc
    10501 agcctcccgg gcaggactca cttatacatc gccggcagat ccttttcaaa aaaggtgaag
    10561 ggatggagat gaaaaagcga gaaatcggtg gcgctgatct gcccgacgta gggggcccag
    10621 tattggcttt cgctggcgtt gcgccagacc agggcccagg tggtcttttt ggactgggca
    10681 ttgtacatca ccgcaggcag gagatagccg gtccagtaat cgctgaccgg gacattctgc
    10741 cagccgacct cggtgagggc ggagatcttg ccgcgcgcct cggccaactc cgccatcatc
    10801 gccagggact tgccgagatc gtcgatgcgc cccttgctgg tcaggagata gtaatccatg
    10861 ccgaggatgt cgacccactc atcgccgggg tagcgcgcca gatactggtc gatggtgttg
    10921 acctcctgcg gcgagatggc gtagaggagg ttgtgcactc cccgggtgtc gcgcagatac
    10981 ccgacggtca ttttccagag ggcgttgtac tcgggcacag tacaggcgcc ggcgccccac
    11041 cacggccagt tgtggttgtg ctcgtgatag ggtctgagga cgatcgggat ggcggtgccg
    11101 ctgctgctct tgaggtcctt gagaaaagcc gcgatctggt caagggtttt cagataagcc
    11161 gcatggtggg attggccggg gaggatatat ttgaccgctg ccgagttgtc ccaggcgctg
    11221 cccccggtgt agggattgtc gaggtgcatg ctgatggtat tgatgccgcc gcgctcgtag
    11281 acctggcgga tccactgctt catggcgtcg aaatagaccc cgtcgagatt ggtctgtttg
    11341 tgaatgtcgc cgaggtccca gccgtagacg gcgggatagt cgccgcagac ctccttgacg
    11401 tcggagcggc ccggcgcggc ccaccagccg acgccgtagg cgaggtcgtc ctgatggccg
    11461 aaaagcaggt tggtctgggc caggctcttc agattattga aaagagccac ggtttcgggg
    11521 gtggcgaggg aatcgacgag gatggtgttg gtcaccaccg gcttttcatc cttcggcccg
    11581 gtcggggact cttttttgcc gcaggcggac aagagagcac agaagaataa aaccagagag
    11641 aatattcgac caagtcgcat attgcttcct ttcaaatgat accccaatat agccacaaga
    11701 agggctcata atcaaggaga attcgattga ataaaagccc tgattttgct attattactg
    11761 tcatgaatat cctctacatc agccagtact ttccgcccga agtgggcgcc acccagaacc
    11821 gggccttcga gatggccgcc aacctggtcc gcctcggcca caaggtcacc atcctgaccg
    11881 aatttcccaa ccaccccggg ggcatcattc cgcccgagta ccgccgccgc tggtatgtaa
    11941 aagacgagct ggcgggcatc cgcgtggtgc gggtctgggt cttcgcccgc ccggtcaaga
    12001 ccttcttcac ccgtatgggc ttttatctca ccttcatggt caatgccgcc ctcgtcggca
    12061 ccctggtccg cggcccctat gacatcgttt atgccacctc tcctccctac ttcgtcgggg
    12121 tgaccgcgct ctggctgagc ctggtcaagc gggcccgctt cgttttcgag gtgcgcgacc
    12181 tctgggtcca gtcggccgcc gaactgggcg aactgagcaa tccccgcctg cttaaaatgg
    12241 ccggttggct cgaccacctc tattaccgca aggcgtggcg catcatcgcc gtaaccaggg
    12301 gcatttacga tgctcttgcc acccgcgggc tggccgccaa actgcacctg atctacaacg
    12361 gcaccaactg cgacctcctc tacgaccgcg gcagcggcaa acgccttgaa ctgggctggg
    12421 agaaaaagtt cgtagtcatg tacgccggta tcctcggcat cgcccagggg ctggaggaga
    12481 tctgccgggt aatcgcccag ctgaaatccg accccgagat ccagttcgcc tttgtcggcg
    12541 caggcccgct caggcagagg atcgagaccc tgcaaaagga gcagggcttg accaacctta
    12601 ccctgctcgg cgagatcccg cgcgagcgca tcgcggacta tttgtctgcc gccgactgct
    12661 gcctggtgcc cctgaagagg cgcgagacct ttcgcggggc cctcccctcc aagatgttcg
    12721 acgtcatggc ctgcagccgg ccgcttcttc tcagcgtcga cggtgaggcg cgcgaactgg
    12781 tggaaaaaat cggcgccggc ctttacgtgg aacccgagaa tgatgacgag ctgcgccagg
    12841 ccatcctcgc actgaaaaat gatcccgcac tcggcaaaaa aatgggcgcg gccggccgcg
    12901 cctttgtcga aaacaatttt tcccgccgcc aggctgcgat cgaactggaa cgggtgcttc
    12961 aggatgtatt gaatgaatca aggagatccg catgaaatcc ggcaagctga tcc
//
LOCUS       DAOPWU010000063        12819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_4979_length_12819_cov_4.622846, whole genome shotgun sequence.
ACCESSION   DAOPWU010000063 DAOPWU010000000
VERSION     DAOPWU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12819
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4979_length_12819_cov_4.622846"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKM23_05125"
     CDS             complement(<1..201)
                     /locus_tag="PKM23_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNY90889.1"
                     /translation="MFTGLIEELGRIEAIEPVGGGKRLAIAAALVPAGLAPGDSVAVN
                     GVCLTVVACNSAGFTAEAVTETL"
     gene            complement(210..1352)
                     /gene="ribD"
                     /locus_tag="PKM23_05130"
     CDS             complement(210..1352)
                     /gene="ribD"
                     /locus_tag="PKM23_05130"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNY90890.1"
                     /translation="MLHLKREQTVAKDGSTAFDQHYMARALELAVKGRGLVSPNPLVG
                     ALLVKGGRVIGEGWHQKLGGAHAEINAIEQAGDEAEQATLYVTLEPCCHQGRTGPCVE
                     RIFRAGIARVVAAMQDPNPLVDGKGFNWLRTRGIEVTTGILEEEAAELNAGYCNFMLH
                     NRPLVTLKVAQTLDGRIATSTGHSKWISSKESRIEAHRLRSQHDALLVGINTVLADDP
                     QLTVRLIEAVSPRRLVLDSSLRVPLDARIIADEMPHRTTILTTPRASKEKISRIQEKG
                     ASVLVLPEDEHGWVDQGALWRKLAEMGCTSVLIEGGNVVATACLKTRAVDRVALFIAP
                     KIIGSGIDAVGDLGIRSLNSAIEIEKIKVRILPVDILVQGQVRYLS"
     gene            1970..4672
                     /locus_tag="PKM23_05135"
     CDS             1970..4672
                     /locus_tag="PKM23_05135"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNY90891.1"
                     /translation="MALIDVEPTLFPESPKEREKLSIPRFFTKAGVHPYDELVWELRS
                     ASITNDKGEVIFDRENIEAPDFWSQTALNIVASKYFHTHKNSSQPETSIKSLIDRVAK
                     TIARWGEEAGYFASLQDARTFEWELTHLLVNQKASFNSPVWFNMGVEDHPQCSACFIN
                     SVEDTMESILDLAKTEGMLFKWGSGTGTNLSPLRSSKEKLSTGGSASGPVSFMRGYDA
                     FAGVIKSGGKTRRAAKMVILNADHPDVVEFINCKANEEKKAWALIEMGYDGSFGGEAY
                     HSVFFQNSNNSVRVTEEFMQAVKMDGPWQTRAVTGGEVVEIHRAVDLFKMMTDAAHVC
                     GDPGIQFHTTINDWHTCPNSAPINASNPCSEYMFVDNSSCNLASLNLLRFVRESGEFD
                     IEAFRRAVSLLIIAQEIIVAGASYPNEQITRNSHLFRPLGLGFANLGALLMYYGLPYD
                     SDEGRALAATLTALMTGQAYLSSAILSSAMGPFAEYDKNREPMLRVIRKHCAALEEIN
                     TALVPAALIDAANEVWNDAHEMGERWGFRNAQVTVLAPTGTIGFMMDCDTTGIEPDIA
                     LIKYKRLVGGGKLKLVNKTITPALHRLGYPDDQVKEIIAYLEREDTIEGAPYLLKEHL
                     PVFDCAFMPLNGNRFIHHMGHVRMMAAVQPFLSGAISKTVNMPNNCTVSDIADVYMEA
                     WKLGLKSIAIYRDGSKRSQPVVTKAGEDQANTENNSFTGQRRRLPDERRSCTHKFSIA
                     GHEGYITVGEYNDGKPGEIFVTMAKTGSTISGLVDAFATSISISLQYGVPLEVLVRKF
                     SYTHFEPYGWTPNPSIHFAKSIADYIFRWLEQKYLANRVSDNEKPDALNGFKQANLTP
                     QPGEPERSFQDQSDAPPCYNCGAIMVRNGACHKCLVCGSTDGCS"
     gene            complement(4761..5111)
                     /locus_tag="PKM23_05140"
     CDS             complement(4761..5111)
                     /locus_tag="PKM23_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY90892.1"
                     /translation="MNFKVDTKNDTYIFRLREERLNAHIAPDLKAQLLLLIGDETNND
                     ILLDLSKVKIVDSSGLGAILLGLRQARANDRRFALLGTHKRVNSLIQIAQVGSVLTSY
                     QDEEEALSGMAAGE"
     gene            complement(5308..6069)
                     /locus_tag="PKM23_05145"
     CDS             complement(5308..6069)
                     /locus_tag="PKM23_05145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY90893.1"
                     /translation="MSTLSVIVITLNEARHIEACLESVAWADEIIVVDAESTDETAAI
                     ARRFTDKVYIEKFRGYSGQKNFALVQAGSEWVLWLDADERVTPELAEEIRTTIAGTPA
                     AAGYEMPRKAFFLGRWIRHCGWYPGHVLRLFRRDCGRFDDREVHETLILNGARGRLRG
                     DLEHYTDESIEHYFWKFNRYTTLAAEELAGRGRRAGMASILFRPLHAFIKMYLLKRGF
                     RDGLPGLILCLLSANYVAAKYAKLAERGAQNWKLS"
     gene            complement(6066..6398)
                     /locus_tag="PKM23_05150"
     CDS             complement(6066..6398)
                     /locus_tag="PKM23_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90894.1"
                     /translation="MKKPAHAIMHGEGGESIPSLHELLALMNERTSRQALELAGGERV
                     SWAGLLFPPLGRFLRIWLLQGLIWRGFHGFRQAWAAALQLMLLRMKIWHLQNHIAAPD
                     GPQGRVRP"
     gene            complement(6395..7441)
                     /locus_tag="PKM23_05155"
     CDS             complement(6395..7441)
                     /locus_tag="PKM23_05155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNY90895.1"
                     /translation="MERILIIKLRAVGDVVLSTIVIDNLRGTFPTAAIDFLTEAAARD
                     VVSGHAQLNEILILERAAIAGMDPRDQLRANLAFLQRVRGRRYDLVFDFFGNPRSALI
                     TRWSGAAWRVGYDYRFRRRAYNVVVPSRADQVHEAEWHLDALTHLGIPVLSRHLSMAQ
                     SEAAAVRAEAFWHEAELEGKRVVALNFSGGWPAKRWPLERFAELAGHVAGRYDARLLA
                     VWGPGELPQAQELAAMAPVPVTLIPETNLKELAALLGRVDLLVSTDSGPMHIAAAMGT
                     PCVALFGPTNHLLQGPYGSNHQIVTNNSLDCLGCNRTACSHTSCMQGLTVEAVLAGVD
                     RAVASLSRRHQERR"
     gene            complement(7703..7999)
                     /locus_tag="PKM23_05160"
     CDS             complement(7703..7999)
                     /locus_tag="PKM23_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90896.1"
                     /translation="MAAKKFVDDDELNEEALPEDEGEEEEEELAEDPDEEDLEEIPED
                     EVEVEVEAEDDEEWDDEFWDDDDEDWEDDEEWDEEDEEDWLDGTPEWEGDDDWE"
     gene            complement(8148..9290)
                     /locus_tag="PKM23_05165"
     CDS             complement(8148..9290)
                     /locus_tag="PKM23_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNY90897.1"
                     /translation="MPRSDQPVKPWNRFEQHFKHRQLAFLESLLGVEERSPAVIDHAA
                     VRRIIVVRQHDQLGDFLLSTPVFKALRGRYPGAEITAVTRSYTAKVAEHNENIDRVVP
                     VYEHGGEWTLQRLREVARLTGARADLTVVLNTVSHSLTSDLIARFATRGVIVGSEHRP
                     FKGTTRNFFYHINASCLEGERHQSQRNLDILRPLGIEGGDLREHIHITPEESAWAREH
                     LRTLGREEGRPLIAVHPGAGKAGNRWPAGHFAAAANTLGAEFGAQIYVTWGRQEGALG
                     DELIAGLERPPLHSLHAEIRRMAALLAEADLFLCNDTGVMHVGAAVGTPLAAVFGPTD
                     PALWKPWGEEFVAVRAEDQRCASVTLEQLLEPARRVLQAGVKKYRI"
     gene            complement(9283..10371)
                     /locus_tag="PKM23_05170"
     CDS             complement(9283..10371)
                     /locus_tag="PKM23_05170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY90898.1"
                     /translation="MKILFANTIQMFGGGEVWMLRALQALQQRGHEVALLCRPGTEVG
                     ERARALGITVHAMPVRGDFGPLTIARAARLLRRHGYQIVLTNMDKELRFMGIAARLAG
                     GCRVVARRGIDYPLKNRLRYRFSYNLLASAVIANSAATRASLLRNAPWLRPERVHVIY
                     NGIDPRPYAAPGEGNFRRSLGVEEGVALVGFVGQLDERKGVEWLLPAFAALHRVFPAS
                     RLVMVGEGPMQEWIVVWCREHGLADAIILAGFSSRIEEVMRDIDLLVLPSLWEGFGIV
                     LIEAMAAGKPCVTTQVSSMPEIVIDGETGRVVPVRDSEALGAALIEVLSDPARAAAWG
                     AAGRRRVEQHFTLERMIDQLESLFKSLA"
     gene            complement(10368..11447)
                     /locus_tag="PKM23_05175"
     CDS             complement(10368..11447)
                     /locus_tag="PKM23_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90899.1"
                     /translation="MAKLKILHIAPTNTSGVPGELVRAERALGHESRLVTLFRDRRGY
                     FEDICLNLAFIDFAGTRWIKRRVSAPERLRVDNRLRIPGEIPPVWRPHSGAESLLVRL
                     RDRMWAPAIRAAIARHGLDRFDVYQLDGGLDFTREPRFIPEMHQRGRRIICCYTGSDL
                     RTRGVIPAIDALSDLNVSVEFDHQALHPQLHHVFFPFDCSRLRMRPEPPVSRPVRIGH
                     APTSRAAKGSDQILPVLRRLSEEEGIEVALIEGMRYEAALALKGSCDIFVDQIGELGY
                     GINSLEALAMTIPAASCLAPGFAAAYPDHPFVEVQAATLYEALLRLIGDGELRRRLGR
                     EGRAWVAAHHDSRAVVRRIHTLAGL"
     gene            complement(11520..12491)
                     /locus_tag="PKM23_05180"
     CDS             complement(11520..12491)
                     /locus_tag="PKM23_05180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY90900.1"
                     /translation="MKYTIVRFTPDDAGGWDEFVWSANNGTLFQTRRFLSYHPEGRFV
                     DHSLSFFSGGRRIAVLPAVDFHDGERRILLSHRGASYGGFMVRDTLGIRESFDLVEET
                     IAHARAEGFDAIDLTPPPQIYFRRPSNYIDFALVQNGFVYRKREISSVIPLDYPREAI
                     LSTFSESSRRAVRRARKQGVLVRESDDYAGFYEILKRNLRLRHNVHPTHTLEELLQVR
                     DLFPERIRLFAAYHGETMVAGVVMFICNPRVVLAFYISHNEEYQQYRGVNALFHDIID
                     WGLSEGYGFLDFGIFTVNEDPNWGLAHFKESFGAQGVFRDSLRLILK"
     gene            complement(12507..>12819)
                     /locus_tag="PKM23_05185"
     CDS             complement(12507..>12819)
                     /locus_tag="PKM23_05185"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821493.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNY90901.1"
                     /translation="SKIEVMRDPVEAVNGADVIYTDTWASMGQEAEAEARRKVFKRYQ
                     VNSKLLEHAARDYIFMHCLPAHRGEEVTDEVMDGPHSVVFDEAENRLHIQKAIMVKLM
                     A"
BASE COUNT         2478 a         4129 c         3908 g         2304 t
ORIGIN      
        1 aagggtctcg gtcaccgcct cggcggtgaa ccccgcgctg ttgcaggcca ccacggtgag
       61 gcagacgccg ttgacggcga cgctgtcgcc aggcgccagc cccgccggga ccagcgctgc
      121 cgcgatggcc aggcgtttgc caccgcccac aggctcgatg gcttcaatcc ggcccagctc
      181 ttcgatcagt cctgtgaaca tgcatcgcct cagctcagat agcggacttg gccctgcacg
      241 aggatgtcga cgggcaggat acggacttta atcttttcga tctcgatggc ggagttgagg
      301 gagcggatgc caagatcccc gacggcgtcg atgccgctgc cgatgatctt gggggcgatg
      361 aacagcgcca cccggtccac cgcccgggtc ttgaggcagg cggtggcgac gacattgccc
      421 ccctcgatca gaaccgatgt gcagcccatc tcagccagct tgcgccagag ggcgccctgg
      481 tcgacccagc cgtgctcatc ctcgggcagc accagcacgc tcgccccctt ttcctggatg
      541 cggctgatct tttcttttga agcccgcggg gtggtgagga tcgtggtccg gtgcggcatt
      601 tcgtcggcga tgatgcgcgc atccaggggc acgcgcaggc tgctgtcgag caccagccgg
      661 cggggcgaaa ccgcctcgat caggcgcacc gtcagctggg gatcgtcggc gagcacggtg
      721 ttgataccta ccagcagggc gtcgtgctgg gaacgcagcc ggtgggcctc gatgcggctc
      781 tccttggagg agatccactt ggagtggccg gtgctggtgg cgatgcggcc gtccagggtt
      841 tgggcgacct tgagggtcac cagcgggcgg ttgtggagca taaagttgca gtagccggcg
      901 ttgagttcgg cggcctcctc ctcgaggatc cccgtggtca cctcaatccc gcgggtgcgc
      961 agccagttga agcccttgcc gtcgaccagg ggattgggat cctgcatggc ggcgacgacg
     1021 cgggcgatgc cggcccggaa gatgcgttcg acacaggggc cggtgcgccc ctggtggcag
     1081 cagggttcga gggtcacata gagggtggcc tgctcggctt catcgccggc ctgctcgatg
     1141 gcgttgatct cggcatgagc accccccagc ttttgatgcc agccctcgcc gatgacccgc
     1201 ccccccttga cgagcagggc gccgaccagc gggttgggac tgaccaggcc gcgccccttg
     1261 accgccagtt caagagcgcg ggccatataa tgctgatcaa aagcggtcga gccatccttc
     1321 gccacggtct gctctcgttt aaggtgcagc atgcattgca cctccggcgg ctgccctcat
     1381 ccgggccgcc cgccgaagaa tcatccaaat gcagggccgg cgccggccgg aacgctgcgc
     1441 agggccccaa actggagaaa aagttacgga taactgggcc ctaaagcaag ctattttcct
     1501 ccagggtagc cccgaaatct ggtttccacc tgactcaccc cgccgtatac tccaatccct
     1561 ccgtacactt ggtttcattc ggatgggact tcgtattggg gggacatcaa cattttcctc
     1621 ccatggagac aacagccgcc gcatggtgat tattccctgc aacttcaatg cctactatag
     1681 cttgacgtgg agcacaaatt aactacaaaa tattgacttt ttcacttgac aatggtccgg
     1741 catttttcta aattgacgtt gcagcaagca gcagcttcgc attgcaacga agtctacgcc
     1801 ccccctgact caagcaaccg aactgtggga tcgggggcct ctgctcaaat caaaataatc
     1861 aaccccaaac attaccgcat tggaaaaaag cgctctctca ggcgtgtggg gaatccttgt
     1921 ctttgtaaca agaggagatg gtatgaagga gtacggcagg tccgagaaga tggcgcttat
     1981 cgatgtggaa ccgacccttt ttcctgagag tcccaaagaa cgagaaaaac tgagcatccc
     2041 gcgttttttc accaaggcgg gagtgcatcc ctacgacgag ctggtctggg agcttagaag
     2101 cgcttccatc accaatgaca agggggaggt catcttcgat cgcgaaaaca tcgaagcccc
     2161 ggatttctgg agccagaccg ccctcaatat cgtcgcctcc aagtattttc atacccataa
     2221 aaattcgtcc caacccgaaa ccagcatcaa gtccctgatc gaccgtgtgg ccaagaccat
     2281 cgcccgctgg ggtgaggaag cgggctattt tgcctccctc caggatgccc gcacctttga
     2341 atgggagctc acccacctgc tggtcaacca aaaggcctcc ttcaacagcc cggtctggtt
     2401 caatatgggg gtggaggatc atccccagtg ctccgcttgc ttcatcaact cggtcgagga
     2461 caccatggaa tccatcctcg atctggccaa gaccgagggc atgctcttca agtggggctc
     2521 gggcaccggc accaacctct cgcccctgcg ctcctccaag gaaaagctct ccaccggcgg
     2581 cagcgcttcc ggcccggtct ccttcatgcg cggctacgac gcctttgccg gggtcatcaa
     2641 atcgggcggc aaaacccgcc gcgccgccaa gatggtcatc ctcaatgccg accatcccga
     2701 tgtcgtcgaa ttcatcaact gcaaggccaa tgaagagaag aaggcctggg cgctgatcga
     2761 gatgggctat gacggcagct tcgggggcga ggcctatcac tcggtctttt tccagaactc
     2821 caacaacagc gtcagggtga ccgaagagtt catgcaggca gtcaagatgg acggtccctg
     2881 gcagacccgc gccgtcaccg gcggcgaggt ggtggagatc caccgggcgg tcgatctttt
     2941 caagatgatg accgatgccg cgcacgtctg cggggacccg ggcatccagt tccacaccac
     3001 catcaacgac tggcacacct gccccaattc agcccccatc aatgcctcga atccctgcag
     3061 cgaatacatg ttcgtcgaca actcgtcgtg caatctcgcc tccctaaacc tgctcagatt
     3121 cgtccgggag agcggcgagt tcgacatcga ggccttccgc cgcgcggtca gcctcctcat
     3181 catcgcccag gagatcatcg tcgcaggcgc cagctatccg aacgagcaga tcacccgcaa
     3241 cagccatctc ttccggccgc tcggcctcgg ctttgccaat ctcggcgccc tgctcatgta
     3301 ctacggtctg ccctacgaca gcgacgaggg gcgcgccctg gccgccaccc tgaccgccct
     3361 gatgaccggc caggcctatc tgagttcggc cattctctcc agcgccatgg gcccctttgc
     3421 cgaatacgac aagaaccgcg agccgatgct gcgcgtcatc cgcaagcact gcgccgctct
     3481 cgaagagatc aacaccgccc tcgttcctgc cgccctcatc gacgcggcca acgaggtctg
     3541 gaatgacgcc catgaaatgg gcgaacgctg gggcttccgc aatgcccagg tgaccgtgtt
     3601 ggccccgacc ggcaccatcg gctttatgat ggattgcgac accaccggca tcgaaccgga
     3661 catcgccctg atcaaataca agcgcctcgt cggcggcggc aagctcaagc tggtcaataa
     3721 aaccatcacc cccgccctcc accggctggg ctaccccgac gaccaggtca aagagatcat
     3781 cgcctacctg gaacgcgaag acaccattga gggtgcgccc tatctgctta aggagcacct
     3841 gccggttttc gactgcgcct tcatgcccct caacggcaac cgcttcatcc atcacatggg
     3901 ccatgtccgg atgatggccg ccgtgcagcc cttcctttcg ggcgccatct ccaaaaccgt
     3961 caacatgccc aacaactgca cggtcagcga catcgctgac gtctacatgg aggcctggaa
     4021 gctggggctc aaatcaatcg ccatctaccg cgacggctcc aagcgctccc aacccgtggt
     4081 caccaaggcc ggcgaagacc aggctaacac ggagaacaac tccttcaccg ggcagaggcg
     4141 caggcttccg gatgaacgcc gctcctgcac ccacaagttc tccatcgccg gacatgaggg
     4201 ctatatcacc gtcggcgagt acaacgacgg caagccgggc gaaatctttg tcaccatggc
     4261 caaaaccgga tcgaccatct ccggcctggt cgacgcattt gccacctcga tctcgatcag
     4321 cctccagtac ggtgtccccc tcgaggtgct ggtgcgcaaa ttcagctaca cccacttcga
     4381 accctacggc tggaccccca atccctccat ccatttcgcc aagtcgatcg ccgactatat
     4441 cttccgctgg ctggagcaga aatacctcgc caaccgggtc tcggataacg aaaaaccgga
     4501 cgccctcaac ggcttcaaac aggccaatct gaccccgcag ccgggcgaac ccgaaagaag
     4561 ctttcaggat cagagcgacg ccccgccctg ctacaactgc ggcgccatca tggtgcgcaa
     4621 cggggcctgc cacaagtgtc tggtctgcgg cagcaccgac ggctgctcat gaaaaccaac
     4681 ggccgataaa aaaatcccgc tcctcctcac ggaagagcgg gattttcttt ttacaggtat
     4741 tactgaagcg gccgatggag ctactcccct gccgccatgc ctgagagggc ctcctcttca
     4801 tcctggtagc tggtcaggac actgccgacc tgggcgatct ggatgaggct gttgacccgc
     4861 ttgtgggtgc cgagcagggc gaagcggcgg tcgttggcgc gcgcctgacg cagcccgagt
     4921 aaaatcgcac cgaggccgga agagtcgacg attttgacct tgctcagatc gagcaggatg
     4981 tcattgttgg tctcatcccc gatcaggagc aggagctgcg ccttgaggtc cggcgcgata
     5041 tgggcattga ggcgctcctc gcgcagccgg aaaatatagg tatcgttttt agtatccacc
     5101 ttgaaattca tgctcacaac cctccgtgga gatcactgcg tccttacatt cttgaatcgt
     5161 taccccctga tctgatgtaa tgtaatggat ttcaaacaaa ggttcaagtg ataattttca
     5221 cagggtactc atgaattccg gctcccaact gccaccctgc gctgcctcac tcattgcatc
     5281 cgggtgggat tctgtttttg gggggcatca agacagtttc cagttctgcg ctccccgttc
     5341 ggcgagcttg gcatatttgg ctgcgacata gttggccgag agcagacaga ggatgaggcc
     5401 ggggagaccg tcgcggaagc cgcgcttgag gaggtacatc ttgatgaagg cgtggagggg
     5461 gcggaacagg atgctggcca tgccggcgcg gcggccgcgg ccggccaatt cttccgcagc
     5521 gagggtggta tagcggttga atttccagaa atagtgctcg atcgattcgt cggtatagtg
     5581 ttccagatcc ccccgcagcc gccccctcgc cccgttgagg atcaaagttt cgtgcacctc
     5641 gcggtcatcg aagcggccgc agtcgcggcg gaagaggcgc aaaacgtggc cgggatacca
     5701 gccgcagtgt ctgatccagc ggccgagaaa gaaggccttg cgaggcattt catacccggc
     5761 cgccgcgggg gtgccggcga tggtagtgcg gatctcctcc gccagctccg gggtcacgcg
     5821 ctcatcggcg tcgagccaga gcacccattc actcccggcc tgcacaaggg cgaagttttt
     5881 ctgcccgctg tagccgcgga atttctcgat atagacttta tccgtgaaac ggcgcgctat
     5941 cgccgcggtc tcgtccgtgc tctcggcgtc gacgacgatg atctcatcgg cccaggcgac
     6001 gctctcgagg caggcctcga tgtgacgcgc ctcgttgagg gtgataacaa tgaccgagag
     6061 ggtgctcatg gacgcaccct cccctgcggc ccgtccgggg ccgcgatatg gttctgcaga
     6121 tgccagatct tcatcctgag cagcatcaac tgcagggcgg ccgcccaggc ctgacggaag
     6181 ccgtgaaaac cccgccagat cagaccctgc agcagccaga tgcgcagaaa gcggcccagg
     6241 ggcggaaaaa gcagtcccgc ccagctcacc cgttctccgc cggcgagttc gagcgcttgc
     6301 cgtgaagtgc gctcgttcat cagggcaagc agttcgtgga gcgaagggat ggattccccc
     6361 ccctccccat gcatgatcgc gtgggcgggc tttttcatct ccgctcctga tggcggcgag
     6421 acagcgaggc caccgccctg tccaccccgg cgagcaccgc ctcgacggtc aggccctgca
     6481 tgcagctggt gtggctgcag gcggtgcggt tgcagcccag acaatcgagg ctgttgttgg
     6541 tgacgatctg atggttggag ccgtaagggc cctgcaggag gtggttggtg ggaccgaaga
     6601 gggccacgca gggggtgccc atggcggcgg cgatatgcat cggtcctgaa tcggtgctga
     6661 cgagaagatc gacccgcccg agcagtgcgg caagctcctt gaggttggtt tcggggatga
     6721 gagtgacggg gacgggggcc atggcggcga gctcctgcgc ctggggcagt tcgcccggtc
     6781 cccagacggc gaggaggcgc gcatcataac ggccggccac atggcccgcc agctcggcaa
     6841 agcgctccag aggccagcgc ttcgccggcc agccgccgct gaagttaagg gccaccaccc
     6901 gttttccctc gagctccgcc tcatgccaga aggcctccgc ccggaccgcg gcagcctcgc
     6961 tttgggccat ggagagatgg cgcgatagca ccggaatgcc gagatgggta agagcgtcga
     7021 ggtgccactc cgcctcatgc acctggtcgg cgcgcgaggg gacaacgaca ttataggccc
     7081 gccggcggaa gcgatagtca tagcccaccc gccaggcggc cccgctccag cgcgtgatca
     7141 gggcactgcg gggattgccg aagaaatcga agaccagatc atagcgccgg ccgcggacgc
     7201 gctgcaggaa ggcgaggttg gcccggagtt gatcgcgggg atccatgccg gcaatagccg
     7261 cgcgctcgag gatgaggatt tcattgagct gggcatggcc cgagaccaca tcccgcgccg
     7321 ccgcttcagt gagaaaatcg atggcggcag tcggaaaggt cccgcgcaga ttgtcgatga
     7381 cgatggtcga aagcaccaca tcgccgacag ccctcagctt gatgatcagt atcctctcca
     7441 cccgtccggg atcgaaggat atccccgcgg ccgtcgtccg ctcctgctgc ttcgtaacct
     7501 gctccttccg ctgttacccc ggcgccgggc gccagggacg aaaaagcccg gaggttctca
     7561 ctccgggctt gtgaggcgat gcggcagccg ccgtatcgcc gccatgctaa accttgccag
     7621 atggcctagg gctcaattcg cccgccggcc ggcgccggca cgcctcaaat cttcagactg
     7681 catatcaagg cggaaaaccg gttcattccc agtcatcatc gccttcccac tccggagtgc
     7741 cgtcgagcca atcctcttcg tcctcctcgt cccactcctc atcgtcctcc cagtcttcgt
     7801 catcgtcatc ccagaactca tcgtcccact cctcgtcgtc ctcggcttcg acttcgacct
     7861 cgacttcatc ttcggggatt tcctcgaggt cttcttcatc aggatcctcg gcgagttctt
     7921 cttcctcctc ctcgccctca tcttcgggga gagcttcctc gttcagctcg tcgtcatcaa
     7981 caaatttttt tgcagccatt tttacctcag tcttaatgca caatatctgg attaaaaaat
     8041 cgcccaaaaa tatggaatgg aaactacaaa tgcaatcctt tttttaggca aattcgccgc
     8101 ggcagcggac cggacctcta atcgttattt gtatatttat aattgtttta aatcctgtat
     8161 ttcttgacac cggcctgaag cacccggcgc gccggctcga ggagttgttc aagtgtgacc
     8221 gaagcacagc gctggtcctc cgcgcgcacg gcgacgaact cctcccccca gggtttccag
     8281 agcgcggggt cggtcgggcc gaaaaccgcc gccagcggag tgccgacggc agcgccaacg
     8341 tgcatgaccc cggtgtcgtt gcaaagaaag agatcagcct cggccagaag ggcagccatt
     8401 ctgcggatct cggcatgaag ggagtggagg ggcggccgct ccagtcctgc gatgagttcg
     8461 tcgcccaggg ccccctcctg ccggccccag gtcacataga tctgcgctcc gaattcggca
     8521 ccgagggtgt tggccgcggc cgcaaaatgc ccggccggcc agcggttgcc ggccttgccg
     8581 gcgccgggat ggacggcgat cagaggcctc ccctcctccc gaccgagcgt gcgcagatgc
     8641 tcccgggccc aggcgctctc ctcaggcgtg atgtggatgt gctcgcggag atcccccccc
     8701 tcgatgccga gcggcctgag aatgtccaga ttgcgctgac tctggtggcg ctccccctcc
     8761 aggcaggagg cgttgatatg atagaaaaag ttgcgcgtcg tgcccttgaa agggcggtgc
     8821 tccgaaccga caataacacc gcgggtggcg aagcgcgcga tcagatcgct ggtgagggaa
     8881 tggctgacgg tgttgagaac gacggtcaga tccgccctgg cgccggtgag ccgcgccacc
     8941 tcgcgcaggc gctgcagggt ccactcaccg ccgtgttcgt agacgggaac gacccggtca
     9001 atgttctcgt tgtgctcggc cactttggcg gtgtagctgc gcgtcaccgc cgtgatctcc
     9061 gctccgggat aacgcccgcg cagggccttg aagaccggcg tcgagaggag aaaatcacca
     9121 agttgatcgt gctggcggac gacgataatc cgtcgcacgg ccgcatgatc gatcacggcc
     9181 ggggagcgtt cctccacccc gagcagggat tccaaaaagg cgagctggcg gtgtttgaaa
     9241 tgctgttcga aacggttcca gggcttcacc ggctgatccg atctaggcaa gggatttaaa
     9301 aagggattcg agctggtcga tcattctctc cagcgtgaaa tgctgctcca cccggcgccg
     9361 tccggccgcc ccccaggccg ccgcgcgtgc gggatcgctg agcacctcga tgagggcggc
     9421 gccaagggcc tcgctatcgc gcaccggcac cacccggccg gtctcgccgt cgatgacgat
     9481 ctcgggcatc gagctcacct gtgtcgtgac gcagggcttg ccggcggcca tggcctcgat
     9541 caggacgatg ccgaagccct cccagagcga gggcagcacc agcaggtcga tgtcgcgcat
     9601 cacctcctcg atacggctgg aaaagccggc caggatgatc gcatcggcca gaccgtgctc
     9661 ccggcaccag acgacgatcc attcctgcat cggcccctcg ccgaccatga cgaggcgact
     9721 agccggaaag acccggtgca gggcagcaaa ggcggggaga agccactcca cccccttgcg
     9781 ctcatcgagc tggccgacaa agccgaccaa agcaacgccc tcttctacgc cgaggctgcg
     9841 gcgaaagttc ccctccccgg gtgcagcata gggacgcggg tcgatgccgt tgtagatgac
     9901 gtgcacccgc tcgggcctga gccagggggc attgcgcagc agagaggccc tggtcgccgc
     9961 cgagttggcg atgacagcac tggcgagcag gttgtagctg aagcggtagc gcaggcggtt
    10021 cttgagggga tagtcgatgc cccgccgggc caccacccgg cagcctccag ccagccgggc
    10081 ggcgatcccc atgaagcgca gctccttgtc catgttggtc aacacgatct ggtaaccgtg
    10141 gcggcgcagc aggcgcgccg cccgggcgat ggtgagcgga ccgaaatccc cccgcaccgg
    10201 catggcgtgg acggtgatgc cgagggccct ggccctttcc cccacctcgg tgccgggccg
    10261 gcagagcagg gccacctcgt ggccgcgctg ttgaagggct tgcagcgcgc gcagcatcca
    10321 gacctcgccc ccgccgaaca tctggatggt attggcaaaa agaatcttca taggcccgcc
    10381 aaagtatgga tgcgcctcac cacggcgcgg ctgtcatgat gcgccgccac ccacgccctt
    10441 ccctcccgcc ccaggcgccg gcgcagctcc ccgtccccga tcagccgcag cagcgcctca
    10501 tacagggtgg cggcctggac ctcgacaaag gggtggtccg gataggccgc ggcaaagccg
    10561 ggggcgaggc agctcgccgc cgggatggtc atagccaggg cttcgaggct gttgatgcca
    10621 tagcccagtt ccccgatctg gtcgacaaag atatcacagc tccccttgag ggcgagggcg
    10681 gcttcatagc gcatgccctc gatcagggcc acctcgatcc cctcctcctc ggagaggcgg
    10741 cgcagcaccg gcagaatctg atcactcccc ttggccgccc ggctggtggg ggcgtggccg
    10801 atgcgcacgg gtcggctgac cggaggttcc ggccgcatcc gcaagcggct gcagtcaaag
    10861 gggaaaaaaa catgatgaag ctgcggatga agggcctggt gatcgaactc caccgataca
    10921 ttgaggtcgc tcagggcgtc gatggcgggg atgaccccgc gtgtgcgcag gtcgctgccg
    10981 gtgtagcagc agatgatcct gcggccgcgc tgatgcatct cggggatgaa gcggggctcc
    11041 cgggtaaagt cgagtccgcc atcgagctga tagacgtcga agcggtcgag gccgtggcgg
    11101 gcgatagcgg cgcggatcgc cggcgcccac atgcggtcgc gcagccgcac cagcaggctt
    11161 tcggcgccgc tgtgaggccg ccacaccggc gggatctcgc cggggatgcg cagacggttg
    11221 tcgacccgga ggcgttccgg cgcggagacc cgccgcttga tccagcgcgt gccggcaaag
    11281 tcgatgaagg ccagattgag gcagatgtcc tcaaaatagc cgcgccggtc gcggaaaagg
    11341 gtgaccagcc ggctctcgtg accgagggcc cgctcggcgc gcaccagttc acccggcacc
    11401 cccgaggtgt tggtgggcgc aatatgaagg attttcagct tggccaataa acgcagcgca
    11461 gtttgggtgg ccgctcggcg ctcacgggcg cctgcggata gctcgaagga ccggaccgct
    11521 cacttgagga tcagccggag actgtcccga aacaccccct gggcgccgaa actctccttg
    11581 aaatgggcca ggccccagtt gggatcttca ttgaccgtga aaataccaaa gtcgaggaag
    11641 ccgtagcctt ccgacaaccc ccagtcgatg atgtcgtgaa aaagggcgtt gaccccgcgg
    11701 tactgctggt actcctcatt gtggctgata taaaaggcca gcaccacacg gggattgcaa
    11761 atgaacatca ccacaccggc caccatggtc tccccgtgat aagcggcgaa gagcctgatc
    11821 cgctccggaa aaagatcgcg cacctgcagc agctcctcca gagtgtgggt gggatggacg
    11881 ttgtggcgca gccgcaggtt gcgcttgagg atctcgtaaa agccggcata atcatccgac
    11941 tcgcgcacga gcaccccctg cttgcgggcg cggcgcacgg cgcggcgcga actctcgctg
    12001 aaggtggaga ggatcgcctc gcggggataa tcgaggggga tgacgctgga gatctcgcgc
    12061 ttgcggtaga caaagccgtt ctggacgagg gcgaaatcga tatagttgct cggccggcgg
    12121 aaatagatct gcggcggcgg ggtgagatcg atggcgtcga atccctccgc ccgggcgtgg
    12181 gcgatggtct cctcgaccag gtcaaagctc tcgcggatgc cgagggtgtc ccgcaccata
    12241 aagccgccgt aggaggcccc gcggtgcgaa agcaggatgc ggcgctcacc gtcgtggaaa
    12301 tcgaccgcgg gaagcacggc gatgcgccgc cccccgctga aaaaggagag ggaatggtcg
    12361 acaaaccgcc cctcggggtg ataggagagg aaacgccggg tctgaaacag ggtgccgttg
    12421 ttggccgacc agacgaactc atcccagccg ccggcgtcat cgggggtgaa acgaacgatg
    12481 gtgtatttca acgcgctctc ctgcctttag gccatcagct tgaccatgat cgccttctgg
    12541 atatgcaggc ggttctcggc ctcgtcaaaa accacggagt gggggccgtc catgacctcg
    12601 tcggtcacct cttctccgcg gtgggccggc aggcagtgca tgaaaatata gtcgcgcgcg
    12661 gcatgctcga gcagcttgct gttgacctgg tagcgcttga agaccttgcg ccgggcctcc
    12721 gcctcggcct cctggcccat gctcgcccag gtgtcggtgt agatgacgtc ggccccgttc
    12781 accgcctcga ccggatcgcg catgacctcg atcttgctc
//
LOCUS       DAOPWU010000064        12774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5002_length_12774_cov_5.427785, whole genome shotgun sequence.
ACCESSION   DAOPWU010000064 DAOPWU010000000
VERSION     DAOPWU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12774
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5002_length_12774_cov_5.427785"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..2185)
                     /locus_tag="PKM23_05190"
     CDS             complement(50..2185)
                     /locus_tag="PKM23_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNY90902.1"
                     /translation="MKRVALSLCLALLLLASASSHAQYFGKNKVQYTNFNWQYVQSEH
                     FNVYYTDGGEEIAAFVLETAEESYRHLQKSFRYQLTDRIKIILHNSHNDFQQTNVDLS
                     EPEESVGGFTEFFKNRVVIPYDGEWEKFRHVIHHELTHAVMLQMLYGSGAQQIITGIT
                     QMQVPLWFIEGLAEYESRGWDIESDMYMRDATVNGYVPDIPYLNAFLAYKGGQSVWNY
                     MVERYGQEKVGEILGKVKVNKNFDRGLKQAIGLEVDDLTKRWHRYLKRKYWPDVENRS
                     EPEEIAKRLTDHRKDGSFVNTSPALSNRGDKLVFKSDKSDFFDIYMMSAIDGRILKRL
                     VKGQRAGNLEELQWLKGPGMSWSPDDKQVVFTARASGGDAIYIVDVKKSKITKTYKLD
                     LDGIYNPSWSPKGDEIAFTGLKNGQSDIYLFNLQTKALRNLTNDLFSDVEPNWAPDGG
                     NLIFASDRGDREAASLPEGFKPQHLNMKNMDIYELAVESGAVTQVVAGPFSERVPVYA
                     ADGKTIAFSSERQGVSNIWLRNLESGEEWPITNVLTGAYQPTWGGNADRLVFISFYFA
                     GYDLYMLKNPLDIKPGDVVVPETKFVENQRKGIKETFEDEPLAGEEPQPEPGEAASDE
                     YRDFVFDDRFAQGELVTQAAKKLFLDSTEYKLPTGRYKVNKYKVELTPDLVYGGVGYS
                     QFYGTQGMTNIMLSDVLGNHRLNIGLGLY"
     gene            complement(2349..3440)
                     /locus_tag="PKM23_05195"
     CDS             complement(2349..3440)
                     /locus_tag="PKM23_05195"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HNY90903.1"
                     /translation="MATLFGRSYTRKELLRHVGDIAQIASVRPVELVNGSERGLRALE
                     FKTGSGFAFTLLQDRALDIYDASFNGVPLHWQGPAGPVAPAFFDPHDIGWLYAMGGGL
                     VTTCGLTHVGPPEIDEEVNEELGMNGRIGSIPAKNVGFGADWQEEEFMLWAVGDVREV
                     SLFGPNLLLRRSIHTRIGQSRLWIKDEVVNDGYEAAPLMLLYQCNAGFPVVAEGSELR
                     AVIENVEPRDEAAAAAMESFDHCDGPTPQAGELVYHIDHDADAKGTVNVALINPEFCA
                     GRGIGLYLSYPKAELPHYTHWKMTGEGTYVVGMLPGNCLPEGRSNARQGSRLRQLEPG
                     ETATFHLEIGVLSSPQERSEFEKRLQTAE"
     gene            complement(3613..4107)
                     /locus_tag="PKM23_05200"
     CDS             complement(3613..4107)
                     /locus_tag="PKM23_05200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY90904.1"
                     /translation="MISIRAMVSHDREIVYEMLQKTGVFSVQAINLAMEYIDAYLFNA
                     DQEEYRVVVGEEAQGQVVVFACFGPAAGTEGTSRIYWLVVTPALQHQGIGSEMLSWIE
                     ERMREEGMRLVVIEVTSEERHAAFVRFFLEQNYRQVGRIRNYFRAGEDMLFYIKYLQA
                     EPSC"
     gene            complement(4392..5747)
                     /locus_tag="PKM23_05205"
     CDS             complement(4392..5747)
                     /locus_tag="PKM23_05205"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY90905.1"
                     /translation="MNPVRILLVDDETDTLQALKLGLEDEGYNILTSPSRNEALTITK
                     NEELDIVITDLKLKDGSGLDLLGYLQEVHPQVPVIIMTAYGSIESTKVALRSGAYDYL
                     AKPFSLADLKRIVAKLVEVIALRKENERLKLLLGRSTEAPVVIGSSPALKKILDLVEQ
                     VAPSRSTILLTGETGTGKEVIASAIHQSSPRADKPFVRINCGAIPENLLEAELFGYER
                     GAFTGAMKQKPGKVELADRGTLFLDEIGELAPAMQVKLLRFIQSGEFERLGGNGALKT
                     DVRIIAATNRNLQEQVEKGLFREDLYYRLNVITITLPPLRERPADIKLLAFYFMQKYN
                     EINNKAIEKIDEEVLSQMMSYPWRGNVRELENMMERAVVLARGSELKMSHFPTLLAGM
                     QGLDLTLGPMVGNSLAEIEKMAIEQTLRYCNHDKQKTAMTLQIGLATLYRKIKEYSLE
                     E"
     gene            complement(5744..7303)
                     /locus_tag="PKM23_05210"
     CDS             complement(5744..7303)
                     /locus_tag="PKM23_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY90906.1"
                     /translation="MQQFAALIKRHHQDLVESSMEALLISPWSSSHPLEIDPARQLFS
                     NILTHLVLDIRQSATRRPGHHLTDYALQLYQKKPHLRQDLHTQLLLLGISRIVVNHFL
                     IRHNAQHNDPDFAARFESLQRLYDTTLISLSHFWGEQYHTLHARDQNLIRELKIVKDG
                     LQRQLNLIYQLLQQSPLGVIALDGDLIVQHWNPMAARLTGFEPPDILDRSVMTLFTPA
                     SRQMLEKKFASSRGYHANLEVYIQRKQGSIFPALISLGRIAGVPQTRFRYILNFQDIS
                     ERAAFQSSVQRLNQLSALSRLSSAIMHDIRNPLNSIGLNTELLEEALESPRPGGEERI
                     AGLVQATKRQLQQLRANLDHYLTYTHLSHLKVEPFDLGLEVGRLVQETRLEAVFQAVT
                     LEYSTSGGACPVVGDWIQLRRVFVNLITNAMEATGASGVIRVRLGRRGRRVSVSITDD
                     GPGIEPEIERSLFSPFFTTKKSGAGLGLFICREIVLAHHGRISVASKRGQGTRFTVSL
                     PLHAHPEDQTP"
     gene            7726..8328
                     /locus_tag="PKM23_05215"
     CDS             7726..8328
                     /locus_tag="PKM23_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HNY90907.1"
                     /translation="MKAKALVTVLVVVFAMAMMLGCAKVPQADLDAAKAALDAAKAAE
                     VDRYLPELFNAVKDSLNSATAEIEKQNSKFALFRSYKKVKELLAKVTADANDAVNQVA
                     VKKEEVKTEVEGLMAQVAPAVENVKGLMKKAPRGKEGRAALEAINSELAAVEASVAEA
                     NTAMTNGDFLTARDKVQANLQKLASLAQELQAAIDKKLGR"
     gene            8511..9707
                     /locus_tag="PKM23_05220"
     CDS             8511..9707
                     /locus_tag="PKM23_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNY90908.1"
                     /translation="MLYEYNRYAKPAKPAPPEKNWFLIINLVLALVLGTISLIVQFSN
                     KPPVARFDEARRDVARARQLQAAVYAPDQLRMAEHYLEQSRLMWASENQRWMYHRNFS
                     RVETMAQKASDMAEYAAMRSVTLRDSLRWVAATGINLTKKRIADIRSQFADMPMEGQV
                     RQQIAFSELAIYESEEAFKRGDFRRAVARYQIASKKIGSAGDEVTQILNGYFVNAPKW
                     RKWVQQTIRWSAEKNEPVIIVDKLAHRCHVYRNGVKTAEFPVELGPNWLGHKRQRGDG
                     ATPEGLYHVKRKKSGHRTAYYKALEINYPNAEDVAQFRAAQARGDIARNAGIGGLIEV
                     HGDGGKGANWTAGCVALRNRDMDTLYSMVGEGTPVTIVGSLRGISTAYSENSAAPDGK
                     GQNTAK"
     gene            9761..10531
                     /locus_tag="PKM23_05225"
     CDS             9761..10531
                     /locus_tag="PKM23_05225"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNY90909.1"
                     /translation="MKRTVRKDFRPQTRESSRQEPDLSFDDYEPEKTEKKIGSALFFG
                     LLFGLLAAFFVFIYAPDLRDALFRLAPRENFVRVDASADMQLYEKELAKAARTVQAKQ
                     KKIAALIPREPWLVIDTSGNQIFLMQDDQLLHKGVCSTGSYVLLRASRNKQWIFRTPR
                     GQFRVLNKMRKPVWYMPDWAFVEEGRPIPPRDSPLRYESGVLGDYALALGDGYLIHGT
                     LYKRFLGMPVTHGCVRLGDEDLEVVAKNMNTGSKVFIY"
     gene            10565..11392
                     /locus_tag="PKM23_05230"
     CDS             10565..11392
                     /locus_tag="PKM23_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90910.1"
                     /translation="MNLLPFFKNRLPAQLVFIFAFVLPVLIVAVPLVILPVQKVVDST
                     SLRMRNMRAAAGGSGGAAPTREMAEQMDALRLEETYANLLLDLAKKDSVMLAINLRDS
                     LVNLLIKGVPVRKCRVSSFRMSQAIPHVARLDSLHTWLYPPFQLLSESATIPKAPIRI
                     MDAPKDTIEAEAMAGQEIPIENNDVHFTLEFNRNLTVRIEQEQPPSSSSEFKKKFAFD
                     MERIFAEARTTIRNLKRKNLPDHKLWIEVVVSREDAKAIYRAIPTRLQMALFLPDAD"
     gene            complement(11447..>12774)
                     /locus_tag="PKM23_05235"
     CDS             complement(11447..>12774)
                     /locus_tag="PKM23_05235"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNY90911.1"
                     /translation="LPGIDLRGTLSGDLLLTHTWKEPRPGGYLELRNGSFIAPYLGRP
                     YAPITARIELEPDRIVLSRLNATGGEGRLQGDGFWRFDLTDKGLEFGEMLLRLKSDRF
                     TAADGPEITLVLDGTVELSGLPAGPLFNGALRIERSRIDLPAFTESPSTLMQADLPLL
                     MVTRGDTASQSYDRKILQPAWLPLVERLRGSIRLDIPRNTWLRSPEMNIEISGKLDLA
                     KEGEQFGLFGSIEIVRGNYDLFSRRFDIKEGRLTFAGGDNLPEMTLQADHLFRSQDKS
                     KHTLSLTASGELRKPLLSFTLDDQPIAESDAVSYLAFGRSFADLTHGERNDLVASQLQ
                     ISGDAFRQMLAGQIAGEVTRSLQQTLDLDVIEFRGDQNWRQSTVVVGKYLTNDLFISY
                     ERQLNFKRTSEVAPEQVTLEYEIIPSLFLQATRGDENSTGIDLIYKWEK"
BASE COUNT         2439 a         3573 c         4135 g         2627 t
ORIGIN      
        1 ggcggggcag atagtagtag gtgagggcat aatcggcgtt gcggaaatct cagtaaaggc
       61 cgaggccgat gttgaggcgg tggttgccga gcacatcgga gagcatgatg ttggtcatgc
      121 cctgggtgcc gtagaactgg ctgtagccga cgccgccgta gaccagatcg ggggtgagct
      181 cgaccttgta cttgttgacc ttgtagcgcc cggtcggcag cttgtattcg gtggagtcga
      241 ggaagagttt tttggcggcc tgggtgacca gctcgccctg ggcgaagcgg tcatcgaaga
      301 caaagtcacg gtattcgtcc gatgctgcct cgccgggttc gggctggggc tcctcgccgg
      361 ccaggggttc atcctcgaag gtctccttga tccccttgcg ctggttctcg acgaacttgg
      421 tctcggggac gacgacgtca cccggcttga tgtcgagggg attcttgagc atgtagaggt
      481 cgtagccggc gaagtagaag gagatgaaga ccaggcggtc ggcgttgccc ccccaggtcg
      541 gctgataggc cccggtcagg acgttggtaa tgggccattc ctcgccgctc tcaaggttcc
      601 tgagccagat gttgctgacc ccctggcgct cgctggagaa ggcgatggtc ttgccgtcgg
      661 cggcatagac cggcacccgc tcgctgaacg gccctgccac cacctgcgtg actgcgccgc
      721 tttcgacggc gagttcgtag atgtccatgt ttttcatatt gaggtgctgg ggcttgaatc
      781 cctcgggcag gctggcggcc tcccggtcgc cccggtcgct ggcgaagatc agattgccgc
      841 cgtccggggc ccagttgggc tcgacatcgc tgaaaaggtc gttggtgagg ttgcgcaggg
      901 ccttggtctg gaggttgaag aggtagatgt cgctttggcc gtttttgagt ccggtgaagg
      961 cgatctcgtc gcctttggga ctccagcttg gattgtagat gccgtcgagg tccagtttat
     1021 aggttttggt gatcttggat tttttaacgt cgacgatata gatggcgtcg ccgccgctgg
     1081 cgcgggcggt gaagacgacc tgtttgtcat ccgggctcca gctcatgccc ggccccttga
     1141 gccactgcag ttcctcgagg ttgccggcgc gctggccctt gaccagccgt ttgaggatgc
     1201 ggccgtcgat ggcgctcatc atatagatat cgaaaaagtc gctcttgtcc gatttgaaga
     1261 ccagtttgtc gccgcggttg ctgagggcgg ggctggtgtt gacaaagctg ccgtccttgc
     1321 ggtggtcagt gaggcgcttg gcgatttcct ccggctcgct gcggttctcc acatcaggcc
     1381 agtatttgcg cttgaggtag cgatgccagc gcttggtgag gtcatccacc tcgaggccga
     1441 tggcctgctt gaggccgcgg tcaaagttct tgttgacctt gaccttgccg aggatctcgc
     1501 ccaccttttc ctggccgtag cgctcgacca tatagttcca aaccgactgc ccgcccttgt
     1561 aggccaggaa ggcgttgagg taggggatgt cggggacgta gccgttgacc gtggcatcgc
     1621 gcatgtacat gtcgctttca atgtcccagc cgcggctctc atattcggca agcccctcga
     1681 tgaaccagag gggcacctgc atctgggtga tgccggtgat gatctgctgg gcccccgagc
     1741 cgtagagcat ctgcagcatg acggcatggg tcagctcgtg atggatgacg tggcggaatt
     1801 tttcccactc gccatcgtag gggatgacga cccggttctt gaagaattca gtaaagccgc
     1861 ccaccgactc ttcgggctct gagagatcga cattggtctg ctgaaagtcg ttgtggctgt
     1921 tgtgcaggat gatcttgatg cggtcggtca gctggtagcg gaaggatttt tgcagatggc
     1981 ggtagctttc ttccgcggtt tcaaggacga aggcggcgat ctcctcccca ccgtcggtgt
     2041 aatagacgtt aaagtgctcg gactgcacat attgccagtt aaagttggtg tactgcacct
     2101 tgttcttgcc gaaatactgc gcgtgcgacg atgcggaggc aagcaggagc agggccaggc
     2161 acagagataa agcgacacgt ttcatagata ctcccgtatc ttggatgagt cgaacagggt
     2221 gctttgcggc accggcagga tcttctggtt taacaccgca gcggtgaaaa ggttgcatgg
     2281 aaaaatcgga gccgggggct ccgccggata ttgcggggtg cgggagcctg cgcagggccc
     2341 gggtcgcctc attccgccgt ctgcagccgc ttttcaaact cgctccgctc ctggggagaa
     2401 gagagcacgc cgatttcgag atggaaggtg gcggtttcgc ccggctcgag ctgtctgagg
     2461 cggctgccct gcctggcgtt gctccgcccc tcgggcaggc agttgcccgg cagcatgccg
     2521 acgacgtaag tgccctctcc ggtcattttc cagtgggtat agtgggggag ttccgccttg
     2581 gggtagctga ggtagaggcc gatccccctg cccgcgcaga attcgggatt gatcagggcg
     2641 acattaaccg tgcccttggc atcggcgtcg tggtcgatat gatagacgag ctcgccggcc
     2701 tggggggtgg ggccgtcgca gtggtcaaaa ctctccatgg ccgcggcggc ggcttcgtcg
     2761 cgcggctcga cattctcgat gacggcgcgc agttcgctcc cttccgcaac gacaggaaaa
     2821 ccggcgttgc attgatagag gagcatgagc ggagcggctt catagccgtc gttgaccacc
     2881 tcgtccttga tccagagccg cgactgtccg atgcgggtgt gaatgctgcg ccggaggagg
     2941 agattgggac cgaagaggct gacctcgcgg acatccccga ccgcccagag catgaactcc
     3001 tcttcctgcc agtcggcacc aaagccgaca ttcttggcgg ggatgctgcc gatgcggccg
     3061 ttcatcccca gctcctcgtt gacctcttcg tcgatctccg gcggtccgac atgggtcaat
     3121 ccgcaggtgg tcaccaggcc gccgcccatg gcgtagagcc agccgatgtc atggggatcg
     3181 aaaaaagcgg gcgcgaccgg accggccggg ccctgccagt gcagagggac gccgttgaaa
     3241 gaggcgtcgt agatatcgag ggcgcggtcc tgcagcaggg tgaaggcaaa gcccgacccc
     3301 gtcttgaact cgagagcgcg caggccgcgc tcgctgccgt tcaccagctc gaccgggcga
     3361 acggaggcaa tctgagcgat gtcgccgaca tggcggagca gctccttgcg tgtataggaa
     3421 cgaccgaata gtgtggccac tatctcctcg ctgggttgaa ttccggaaca gggcgccccc
     3481 ttcccggcac gggagagcgg cgccgatgca tttaaaacaa tgaattaaca tagctaaaaa
     3541 gcgggtaaaa gtcaagcaac aaaaaggggg agagccggga gcgccgggtg gcgcaaccgt
     3601 ggggaagagg cctcagcagg agggctcagc ctgcaggtat ttaatataaa agagcatgtc
     3661 ttctccggcg cggaagtagt tccggatccg tcccacctgc cggtagttct gctcgaggaa
     3721 aaaacggaca aaggctgcgt ggcgttcctc agaggtgacc tcgatgacga ccaggcgcat
     3781 cccctcctcg cgcatgcgct cctcgatcca ggagagcatt tcgctgccga tgccctgatg
     3841 ctggagggcg ggggtgacca ccagccagta gatccggctg gtgccctcgg tgccggcggc
     3901 ggggccgaag caggcgaaga ccaccacctg gccttgggcc tcctcaccga cgacgacccg
     3961 gtactcctcc tggtcggcat tgaaaaggta ggcatcgata tattccatgg cgaggttgat
     4021 cgcctgaaca ctgaagacgc cggtcttttg cagcatctca tagacgatct cccggtcgtg
     4081 cgacaccatg gcgcggatcg agatcatggc atgtactctg tctggtcagc ggaacaaggg
     4141 gcgcggcatg gcagtgcgcc ccggcgaatc taattggcaa ataatgggta aacaataaca
     4201 atgccagaaa ttcagcagaa aatagacatt gataataatg gagataggct gtccttgaga
     4261 ggggagagcg ggccggggcc ggcctttttt ttcgatatga aaaagaggga caacataacg
     4321 ggcaaaatat tctcattatg agaatatggt gcagggcctt gccgaagggc ttcttaaaat
     4381 gataattcac ctcattcctc gaggctgtac tccttgatct tgcggtaaag ggtcgccagg
     4441 ccgatctgca gggtcatcgc tgtcttttgc ttgtcatggt tacagtaacg cagggtctgt
     4501 tcgatggcca tcttttcgat ctcggcgagc gagttgccca ccatcgggcc gagggtgaga
     4561 tcgagcccct gcatgccggc cagaagggtg gggaagtggc tcatcttgag ttcgctgccc
     4621 cgggccagca ccaccgcccg ttccatcatg ttttcgagct cgcgcacgtt gccccgccac
     4681 ggatagctca tcatctgcga gagcacctcc tcatcgattt tctcgatcgc cttgttgttg
     4741 atctcgttgt atttttgcat gaaataaaag gcgagcagtt taatgtcggc ggggcgttca
     4801 cgcaggggcg gcagggtgat ggtaatgacg ttgaggcggt agtagagatc ctcacggaag
     4861 aggcctttct ccacctgctc ctgcaggttg cggttggtgg cggcgatgat gcgcacatct
     4921 gttttgagcg cgccgttgcc gccgaggcgc tcgaactctc cactctgaat gaagcggagg
     4981 agcttgacct gcatggcggg ggccagctcg ccgatttcgt cgaggaagag ggtacccctg
     5041 tcggccagtt ccaccttgcc gggtttttgc ttcatcgccc cggtgaaggc cccgcgctcg
     5101 tagccgaaca gttcggcctc gagcaggttt tcggggatgg cgccgcagtt gatgcggacg
     5161 aagggtttgt cggcgcgcgg actcgactga tggatggccg aggcgatgac ctccttgccc
     5221 gtgccggtct cgccggtgag caggatggtg ctgcgcgagg gggcgacctg ttcgaccaaa
     5281 tccaggattt ttttcaaggc cgggctgctg ccgatcacca ccggggcctc ggtgctgcgg
     5341 ccgagcagca gcttgaggcg ttcgttctcc ttgcgcaggg cgatgacctc gaccagcttg
     5401 gcgacgatgc gtttgaggtc ggcgaggctg aagggtttgg ccagatagtc ataggccccg
     5461 ctgcgcaggg caaccttggt cgattctatg gagccgtagg cggtcatgat gatcaccggc
     5521 acctgcggat ggacctcctg gagatagccg agcagatcca gtcccgagcc gtccttcagc
     5581 ttcaggtcgg tgatgacgat gtcgagctcc tcgtttttgg tgatggtgag ggcctcgttg
     5641 cgcgaggggg aagtgaggat gttatagccc tcatcctcca ggcccagctt gagggcctgc
     5701 agggtatcag tctcatcatc gacgaggaga atacggacgg gattcatggg gtttggtcct
     5761 ccggatgggc atgaagtggc aggctgacgg tgaagcgggt gccctgaccg cgttttgagg
     5821 cgacgctgat gcggccgtgg tgggcgagga cgatttcgcg gcagatgaag aggccgagcc
     5881 ccgccccgct ctttttggtc gtgaagaagg gggagaagag gctgcgttcg atttcgggct
     5941 cgatgcccgg tccgtcgtcg gtgatgctga cggagacgcg gcggccgcgg cgcccaagcc
     6001 gcacccggat aacgccgctc gccccggtcg cctccatggc gttggtgatc agattgacaa
     6061 agacccgccg cagctggatc cagtcgccca ccaccgggca ggcccccccg ctggtagagt
     6121 attcgagggt gaccgcctgg aagaccgcct ccagcctggt ctcctgcaca aggcggccga
     6181 cctcgagacc gaggtcgaag ggctccacct tgaggtgcga gagatgggtg taggtgagat
     6241 agtgatcgag gttggcgcgc agctgctgca gctgccgctt ggtcgcctgg accagcccgg
     6301 cgatgcgctc ctcccctccg ggccgcggcg actcgagcgc ctcctcgagc agttcggtgt
     6361 tgaggccgat cgaattgagg ggattgcgga tatcgtgcat gatcgcgctg ctcagacgcg
     6421 agagcgcgct gagctggttg aggcgctgca cgctcgactg aaaggccgcg cgctcgctga
     6481 tgtcctggaa attgaggatg tagcggaaac gcgtctgcgg caccccggcg atgcgtccca
     6541 gggagatcag ggccggaaag atcgaaccct gcttgcgctg gatgtagacc tcgaggttgg
     6601 cgtgataccc ccgcgacgag gcgaactttt tctcgagcat ctgccggctg gcgggggtga
     6661 aaagagtcat gacgctgcga tcgaggatgt cggggggctc aaagcccgtc agccgggcgg
     6721 ccatcggatt ccagtgctgc acaatcagat cgccatccaa ggcaatcacc ccaagggggg
     6781 actgctgcag gagctgataa atgaggttga gctgacgctg gaggccgtcc ttgacgatct
     6841 tgagctcgcg gatcagattc tggtcgcggg catggagggt gtgatactgc tccccccaga
     6901 agtggctgag gctgatcagg gtggtgtcgt agagccgctg cagggattcg aaccgggcgg
     6961 cgaagtcggg atcgttgtgc tgggcgttgt ggcggatgag aaaatggttg acgacgatgc
     7021 gcgatatgcc cagcagcagc agttgggtat ggaggtcctg gcggagatgg ggctttttct
     7081 ggtagagctg cagggcataa tcggtgaggt gatggccggg gcgcctggtc gcactctggc
     7141 ggatgtcaag aaccagatgg gtgaggatgt tggagaaaag ctggcgcgcc ggatcgattt
     7201 cgaggggatg gctgctgctc cagggggaga tgagcagggc ttccatcgag ctctcgacca
     7261 gatcctgatg atggcgtttg atgagggcgg cgaactgctg catgggtcag ggcctctggt
     7321 tttatcattt taagagatca aatttacgac aattttatca atttatcaaa ttgagaatca
     7381 aatggccgtt gaattttgct tttttttcat attgaaaaaa gggagcggca agtaaatcgg
     7441 cggcgcgaaa accggctatg taatcttaat aaaaacaaga tatggcctga tccaaaaagt
     7501 ttggcatcag atttgtatag ggcagggtag aaaaagatac ctttccctga ttttgcaagc
     7561 gcgcctggcc tgatctcctg cgagaaaggg agaggccggc aagaaaaaac gtcacaaaga
     7621 tattttctct tgtttttttg caaattatac cctaaattgt ctgaagttta cagagggaag
     7681 gtttctgtgc accaatttac acacccattt aaggaggttg ttagaatgaa ggcaaaagcg
     7741 ttagtgactg tgctcgtcgt cgtctttgcg atggccatga tgcttggttg cgccaaggtt
     7801 ccgcaagctg atctggatgc cgcgaaggcc gctctggatg ccgcgaaagc tgccgaagtc
     7861 gaccggtatc tgcccgagct gttcaacgcc gttaaggatt ccctgaactc cgccacggcc
     7921 gagatcgaga agcagaattc caagttcgcc cttttccgct cctacaagaa ggtcaaggaa
     7981 ctgctggcca aagtgactgc cgacgccaat gacgccgtca atcaggtcgc tgtgaagaag
     8041 gaagaggtca aaacggaagt tgaaggcctg atggctcagg tcgccccggc tgttgagaat
     8101 gtcaagggtc tgatgaagaa ggccccgcgt ggcaaagaag gccgtgccgc cctcgaagcc
     8161 atcaacagcg aactggctgc tgtcgaagcc tccgtcgccg aagccaacac cgccatgacc
     8221 aatggcgatt tcctgaccgc tcgcgacaag gtccaggcca atctgcagaa gctggcgagc
     8281 ctggctcagg agctgcaggc tgcgatcgac aagaagctcg gccgctagtc cttcactctc
     8341 cactgagtgt tgattacaaa cctcagtccg tgcaagcagc gcttcgcggg ctgaggtttt
     8401 ctgttttcaa tccgtgccgg ggttgttctt tgacccaagc ggtggatgcg tctctgactt
     8461 cctttcgctg gccccgattt tctggtattc tggcattaat tgagtgaagc atgctgtacg
     8521 agtataaccg ttacgccaaa ccagccaagc ctgccccgcc cgaaaaaaac tggtttctga
     8581 tcatcaatct tgtcctggcg ctcgtcctgg gcaccatcag tctgatcgtg cagttttcca
     8641 acaagccgcc ggtggcccgc ttcgatgagg cgcgcaggga tgtcgcccgg gcccgccagc
     8701 tccaggccgc tgtctatgcc ccggaccaac tgcgcatggc cgaacattat ttggagcagt
     8761 cccgcctgat gtgggcttcg gagaatcagc gctggatgta tcaccgcaat ttcagccgcg
     8821 tcgagaccat ggcgcaaaag gcgagcgaca tggctgaata tgcggcaatg cgttcagtga
     8881 ccctgcgcga cagcctgcgc tgggtagcgg cgaccggcat caatctgacc aagaagcgga
     8941 tcgccgatat ccgcagccag tttgcggata tgccgatgga gggtcaggtg cgccagcaga
     9001 tcgccttcag cgaactggcc atctatgaga gtgaagaggc cttcaagcgc ggggattttc
     9061 gccgcgccgt cgcccgctat cagatcgcct ccaaaaagat cggcagcgcc ggggatgagg
     9121 tgacgcagat cctcaacggc tattttgtca acgcccccaa atggcgcaaa tgggtgcagc
     9181 agaccatccg ctggtcggcc gagaagaacg agccggtgat catcgtggac aagctggccc
     9241 atcgctgtca tgtctaccgc aacggcgtca aaacggcgga gtttcccgtc gagctcgggc
     9301 cgaactggct ggggcacaag cggcagcgcg gcgacggggc gaccccggag gggttatacc
     9361 acgtcaagcg caagaaatcg gggcatcgca ccgcctatta caaggccctg gagatcaact
     9421 atcccaatgc cgaggatgtg gcccagttcc gcgcggcgca ggcgcgtggc gatattgctc
     9481 gtaacgccgg catcggcggc ctcatcgagg tccacggtga cgggggcaag ggcgccaact
     9541 ggacggcggg ctgcgtcgcc ctgcgcaatc gcgatatgga taccctgtac agcatggtgg
     9601 gcgaggggac cccggtcacc atcgtcggct cgctgcgggg tatcagcacc gcctacagtg
     9661 aaaacagcgc ggcacccgac ggcaaagggc agaacacggc caaataaccc ggcaccagcg
     9721 ggggcgccgg ttgcaggtta acgtcccttt tacaggcact atgaaacgca cagttcgcaa
     9781 ggatttccgt ccgcagacca gggagagctc caggcaggaa cccgatctct ccttcgacga
     9841 ttatgagccc gaaaagaccg agaagaagat cggttcggcc ctatttttcg gccttttgtt
     9901 cggcctcctg gctgcctttt ttgtcttcat ctatgccccg gatttgcgcg atgccctctt
     9961 ccgcctcgcg cccagggaga actttgtccg cgtcgacgcc agcgccgata tgcagctcta
    10021 cgagaaagaa ctggcgaaag cggcccggac cgtgcaggcc aaacagaaaa agatcgcagc
    10081 cttgatcccc agggagccct ggctggtcat cgacacctcg ggcaaccaga tctttctcat
    10141 gcaggatgac cagctgctgc acaagggggt ctgttccacc ggcagctacg tcctgctgcg
    10201 cgccagccgc aacaagcagt ggatcttccg caccccgcgc ggccagttcc gggtgctcaa
    10261 caagatgcgc aagccggtct ggtacatgcc ggactgggcc tttgtcgagg aggggcggcc
    10321 gatcccgccg cgggactccc ccctgcgcta cgagtctgga gtgctgggcg actatgccct
    10381 cgcccttggc gacggctatc tcatccacgg caccctttac aaacgctttc ttggcatgcc
    10441 ggtcacccac ggctgtgttc gtctggggga tgaggatctc gaggtggtcg ccaaaaatat
    10501 gaataccggt tcgaaggtgt tcatttacta agtcgctttc tgtcccattg gagagagttc
    10561 atccttgaat ctgctgccct ttttcaagaa tcgcctgccc gcgcagctgg tctttatctt
    10621 cgcctttgtg ctgccggtgc tcattgtcgc cgtgccgctg gtgatcctgc cggtgcaaaa
    10681 ggtggtcgat tccacctccc tgcggatgcg caatatgcgc gccgccgcgg gcggcagcgg
    10741 cggggcggcg cccacgcgcg agatggcgga gcagatggat gccctccggc tcgaggagac
    10801 ctatgccaat cttctccttg atctggccaa aaaggattcg gtcatgctgg ccatcaatct
    10861 gcgcgacagc ctggtcaatc tgctcatcaa gggggtgcct gtgcgcaagt gccgggtcag
    10921 ctccttccgc atgagccagg ccatccccca cgtcgcccgg ctcgactcac tccacacctg
    10981 gctctacccc cccttccagc tcctgagcga gtccgccacc attcccaagg cccccatccg
    11041 catcatggat gcccccaagg acaccatcga ggctgaggcc atggccggcc aggagatccc
    11101 gatcgagaac aacgatgtcc atttcaccct ggagttcaac cgcaacctca cggttcgcat
    11161 cgagcaggag cagccgccga gcagcagcag tgagttcaag aaaaaattcg cctttgatat
    11221 ggagaggatt tttgcggagg cccggacgac gatccggaat ctgaagcgca agaatttgcc
    11281 cgatcacaag ctgtggatag aggtggtggt cagccgcgag gacgccaagg cgatctatcg
    11341 ggccatcccc accaggctgc agatggctct tttcctgcct gacgccgatt agccccttcc
    11401 ccctgcggcc ccgtcctttt acggctgcag gccttcccct gtttttctat ttttcccact
    11461 tgtaaatcag atcgattccc gtactgtttt catctccgcg cgtggcctgg agaaaaaggg
    11521 aggggatgat ctcgtattcc agggtcacct gttcgggcgc cacctcgctg gtgcgcttga
    11581 aattgagctg gcgttcgtag ctgatgaaaa ggtcgttggt caggtatttg ccgacgacca
    11641 ccgtcgactg ccgccagttc tggtcgcccc tgaattcgat cacatccagg tcgagggtct
    11701 gctggaggga gcgggtcacc tccccggcga tctgtccggc gagcatctgc ctgaaggcgt
    11761 cgccgctgat ctgcagctgc gaggctacca ggtcgttcct ctcgccgtgg gtgagatcgg
    11821 cgaagctgcg cccgaaggcg agataggaga ccgcgtcgct ctcggcaata ggctggtcat
    11881 caagggtgaa gctgaggagc ggcttgcgca gctcgcccga ggcggtgagg ctgagggtgt
    11941 gtttgctttt gtcctgtgag cggaagaggt ggtccgcctg cagggtcatc tccggcagat
    12001 tgtccccgcc ggcgaaggtg agacggccct ccttgatgtc gaagcggcgg ctgaagaggt
    12061 catagttgcc gcggacgatc tcgatggagc cgaagaggcc gaactgttcg ccctccttgg
    12121 cgaggtcgag tttgccggag atctcgatgt tcatctccgg gctgcgcagc caggtgttac
    12181 gggggatgtc aaggcggatc gagccgcgca agcgttcgac gaggggcagc caggccggct
    12241 ggaggatttt gcggtcatag ctctggctgg cggtgtcgcc gcgagtgacc atgagcaggg
    12301 gaaggtccgc ctgcatcagg gtggaggggg attcggtgaa ggcgggcagg tcgatgcggg
    12361 accgttcgat gcgcagggcg ccgttgaaca gcggcccggc cgggaggccg gagagctcca
    12421 cggtgccgtc gaggacgagg gtaatctcgg ggccatcggc ggcggtgaaa cggtcgcttt
    12481 tgaggcgcag cagcatctct ccgaactcca gacccttgtc agtgaggtcg aagcgccaaa
    12541 agccatcgcc ctgtaggcgg ccctcgccgc cggtggcatt caggcggctg agcacgatcc
    12601 tgtcgggttc aagctcgatg cgggcggtga tgggtgcgta gggtctgccg agataggggg
    12661 cgatgaagga gccgttgcgc agttccaggt agccgccggg ccgcggctct ttccaggtat
    12721 gggtgaggag gagatcgccg ctgagggtgc cgcgcagatc gataccgggc agaa
//
LOCUS       DAOPWU010000065        12764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5008_length_12764_cov_4.743331, whole genome shotgun sequence.
ACCESSION   DAOPWU010000065 DAOPWU010000000
VERSION     DAOPWU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5008_length_12764_cov_4.743331"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKM23_05240"
     CDS             complement(<1..466)
                     /locus_tag="PKM23_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90912.1"
                     /translation="MIPDCQQLGSDRLLHRWGDIFSPPGITNFWGVCQADLDITGVRS
                     LNFPPFTNGDKVTASLFLDGWYFAALNHPVAFAWYPDRIVRESEHEGLELTSEMILSF
                     GRTALLIRLEIRNCGGAERETVVKFGTAAAITQNRGTGKLYIPPSEEDNRVTV"
     gene            complement(479..1615)
                     /locus_tag="PKM23_05245"
     CDS             complement(479..1615)
                     /locus_tag="PKM23_05245"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HNY90913.1"
                     /translation="MIRIASIRDLGPQFRENSHRMVGQDGAYSIPTQAGLLWFFGDTL
                     IGARRPGESLWYPDGKPVGHADMSGLAGIEHMINNTGLLSSTEVGPAGIAGYRYILDG
                     RGGIKPLIPLERGEDPDWIRVWCLHGIEVEGKICLFFVKVETVEEGIFPVNFRILGSG
                     LAAGEAREWSFTRILHNGSDIWWPAEQPHFASAVLTPVGDWLYLYGVLQGEDKIQRCY
                     LARVRREEITRLDRYEYLCAAAPGSERGLPRAADFGCAPDEPTGPAWSSRIEEAVPLF
                     DGMPNEQSVSWNPWLGCYLAVHSLDLSGKIVARTAPEPWGPWSTPAELYQVRCSHPAH
                     LPYPQLIYAGKEHPALAREGGRIIYVTYIEFEEYYPHLLEITLY"
     gene            complement(1626..5075)
                     /locus_tag="PKM23_05250"
     CDS             complement(1626..5075)
                     /locus_tag="PKM23_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90914.1"
                     /translation="MRRLILCLALIPLAAAAQRAETLPLSVDGSLQAWLTNGPFEIRT
                     VGFGDLSDDAPLDEVQVAPQSGGRVRNSGLAEGESPWLYLSTRDNGFTDLNPFYGWKT
                     LATPEKVWYAKIVYAWAEMVSERSREALLSFGGNTVTRIILNGETVYQSLKECNAIPD
                     NFAVPVTLKKGSNRLLIRTANTHRNHAVDFFDPLRFEWGFYCRLLNPDTKAPLTDVSQ
                     RIEAAGLQNAFTLTPTFFCKKKRGGLVQKYLLEIHSSQTAKIPAFFSLPYVDVKFTLN
                     DITFGVNQREIYLPEVEAPVKVPAVLEMAGESFSRRVELKSLPHYELYFMPLTHMDIG
                     YTNPQPVVIERQLQTLDQAVQKCAADSSFRWTIETMWLLENYRRARSEEAFARLIALI
                     KAGRIAVSPIYTNPYTGWVSEAEMAASFRFAEEYRSRYGLDYFAAVYNDVPGQSWALP
                     QYLRKAGVRFLANGINELFADYKFQKSLPKVFHWAGSSSDTLLLYLTEAYIEGARYGL
                     EREPEATACRIWHRVNNLLRRDYPYSKILISGAFSDNSGIALAQYENARRWNNLYAWP
                     RFVIATLDDFGRALCAENPALLETRRGDWTSDWDILYQGETRRMTAYRGVQNQLPGVE
                     VLSALSSALHPETRTGAGEIDAVYEDLLSFSGHGSGLEYGLGSKEENLYTDAVRDDHV
                     RNAAQRSRAMRERLLYRLTAPKESFESQGIMVFNTLSWERSAPVEIDFPATDSNSYAV
                     IDLASGEKLPSHRSGERLSFIARDLPGIGSRQYELVTRPAPAAAGPQPDNSRPAAKPV
                     RAHAALPPASGPIIENEHYRISATNKGIEILEAATGRPLLNSAALPSFLPLRKRFQLQ
                     ESFTPIAGWAGEPRISRNPAFAELCWGYTGDIFRTITFRLWNGLNRIDISVEIDLEAL
                     PKCEQTEEYGLAFALDAPGSGIRCETLGGLTALGDRFSGVGHTFFSIRRALGAAAAER
                     TLLLASPDCRIFALDTLNNAGTVIANLVNNFPESWNRNEQNSGRLTFRFRLASTTARQ
                     ADIAAFGWEAAAEPLVRRSWFTRAEPIQQYLASSDPWIKIISLRPAGEENGRYELLLQ
                     NSDREASREMTLTSALWLGGATVTEQDLLGRSLRIIPVQQNAITLCLPANGLMRVSIE
                     KQR"
     gene            complement(5151..7019)
                     /locus_tag="PKM23_05255"
     CDS             complement(5151..7019)
                     /locus_tag="PKM23_05255"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HNY90915.1"
                     /translation="MVLLMPLLLSSCKITQITQPQSAELNSEITVRLTVQDGADANVH
                     KGVLGLLLPSDWRVLEAVYSSTVGDGDLEYSTAWTDSLAACYPPAEFEGEMKWVGLLS
                     DQGYAPAAAIQIDIEVRLQTGASPGCFKLGYLVTKATTNLLCSGNDGWAPFSYPNPIA
                     LPAGASCPGEYEVRRSEAWEQLVTRSSGWTGADGIYSIPLDGSEQPHGQDHLIIFSDT
                     FIGQVDASGRRVNSTIVNNTLAHLQGSRPDPGRIAFMWDQSGASPRAVFTPATPTAVA
                     NDFYWLMDGVKIDSLIHLFALRLHTTGSGAFDWKLTGVVLLSFTLDENHYPAGVQQRD
                     LPFFMKDGELTAVLGQAILPNSAASGNSRADGYIYIYGPRDDSSGKSLVAGRVRPEEL
                     LDFTAWRFWDGSGWNSDHTASASITNRISQEFSLSELNGRYILVCQIGSAVAVRFGDT
                     PVGPFEMYREIYKAPEVEISDNVFIYNAKAHPSLSDADSLLITYNVNTLDFGENLRVA
                     DIYHPRFIKLATAGIGSGVAEPVPPPAAFALLQNYPNPFNPATEIAFEINERTEVTLR
                     IHNARGQLVETLLESRSFAPGRYTTRWQPNNLGSGLFFYTLETYGRRETRKMMLIQ"
     gene            complement(7043..7939)
                     /locus_tag="PKM23_05260"
     CDS             complement(7043..7939)
                     /locus_tag="PKM23_05260"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY90916.1"
                     /translation="MLLAQYDYGFDFAKTGSGGLQFLKIGVGARESAMGEAVAGDVKD
                     VNAVFWNPAGLAYATRYQASFSHNTWLVNSRQMAAAVAIPLKTFTVALSALSFAIDDF
                     EETTVTSPEGTGRMVSAGDLMVGLSAARMFTDKLSIGGQVKYVREKLDDYSINNLLFD
                     IGALYYTGFRSLRLAFSLQHFGPDMELVDQEYRTPLLFRINATDELYKSEEMAWTLGA
                     ELVHPTDNVEIVNIGTELQLHQLLFLRAGYRFNTDEGKLACGFGLVTPAMAGVNLRLD
                     YSFVQSERVFDDIHRFTAGFTF"
     assembly_gap    8068..8167
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8168..9957)
                     /locus_tag="PKM23_05265"
     CDS             complement(<8168..9957)
                     /locus_tag="PKM23_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90917.1"
                     /translation="MKYKLLCFMLLPLSLFAQARKADFRIHNRGVLWETMKDDGTIGA
                     PDPVNRFQYYPSMDWPGGPETMVKDDQRSYMAGAGLWIGGYHEDGSLFYTENGPFTYV
                     DQGTFYPLEVRNNFIGEPGYDPAEAEQVISMRLTTTHNIEVKRTSRAWSFPGNDNFIL
                     LEYEFINRNAGRVTDLFLGFPYLIRPSYQDFVVHNGWGDDFNRTDELVRYDDDYRLLY
                     AWDDTPNYTLPTDVGNYWDDRSELRTTGYAGYALLQATPAKGSRFQPAVVLWAQLLNN
                     ERYFSLTSSAAADLYKILSGQDKSLQADEEAHLTPMMLMSLGPYDLAKDESVRIVLVE
                     AVAGISLRDALKGLDAQPMLEDGEEMLQETIFAAQKLLAAGYAMKKLPPPSPALQIIP
                     VPENQSITLNWDPVDQSWVDPLTGSGDFLEYRVYRSSLAFIGPYDLLKAIRPGRATDV
                     TRYFDTMKNKWIFEDNTISLGAGYFYAVTAVDAEGNESALTNRNFMPVYAIRSPAADA
                     LSVTVFPNPFRITSGFPVSGAENSIVWTNLPAECTIRIYTSNGELIKTMEHNSPFSGE
                     ETWDQLTDARQRPAPGIYFWTVESPVGHAKG"
     gene            complement(10137..>12764)
                     /locus_tag="PKM23_05270"
     CDS             complement(10137..>12764)
                     /locus_tag="PKM23_05270"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNY90918.1"
                     /translation="DVTASIQNIGIAEFARIPCLVSRMGMRDTKQGLMAQAGVFLDPI
                     PVAGGLGSAGKGETRYSVRGGSQDEVKWFIDGARVSNLLLGRADWGGSFTNVPMQAIK
                     EVQVMTGGFTAEYSDAQSGVVSIVTREGGDALHGSVDFSYGFAGQHHFGPYLYDRASH
                     KEFRDNTLPDGSLNPEWWTTTRQAQVYDYRKIPDYTAALSLDGPLFRLKGAPVTFFSA
                     LNLRKQAYTLPHPRDSRDAGNGMFNIAWNPKNIKMKLTGFYNTDAHSTLQENGDFTNQ
                     AKYYRGWGSLLNTDTWLLNYGFTQFLNERLFYELKLATYWSHFKEKPSDFSLVGASQK
                     PTLWGFHRFDGYESEPFDNYSPVLYNDILTGDLSLTGNVNWQFDANNLLKSGFELRYN
                     TYDERETYRIPSFSRDKSLWLNRGLMERYHPLQAALFLQDKMEFESMILNVGVRYDYY
                     DPNINWFEPTTLYNLAIDPAFDPALDPDKDQVDANGHVKYAFDNVLDKARKPAKAWHM
                     ISPRFGVSFPISDNSLLHFNYGHYYQMPPLDQSFEIGYFRPEYIVKNILAERTAAAAE
                     GREERHIPSNDGDAERVVGFTNQPLKPQKTIQFEAGFKQSFEGIAVLDLVAYYKDVFD
                     QTQERIGLFDHHIYGWDPFSGVTTPNASYQTYLTGDYGDSRGIEAALHSLFSKVLFFD
                     VNYSFSRATEGRATPGVIRYDGEGNIDYTWDSDVNKRIPIEKSFSRPHVLRVNSYLRY
                     PASSSARLIDRVFRESTMSLLFNYYSGQSFTYLQATDSPDTYNNYRFPAIHTFDLKLE
                     KQFPLWGGHTTSFYMNVTNLFNRKNLRSYGDALFDSEATKNFIEKGTVSTVDADGYDI
                     GWQNYFETRRIYMGVKYSF"
BASE COUNT         2376 a         3490 c         4322 g         2476 t
ORIGIN      
        1 cgacggtaac ccggttgtcc tcctccgagg ggggtatata aagcttgccg gtgccgcggt
       61 tctgcgtgat ggcggcggcg gtgccgaact tgaccacggt ttcgcgctcc gcccccccgc
      121 agttgcggat ttcgaggcgg atgagcagag cggtccggcc gaaggagagg atcatctccg
      181 aggtgagctc gagcccctca tgctcggact cacgcacgat ccggtcgggg taccaggcga
      241 aggcgacggg atggttcaga gcggcaaagt accagccatc cagaaaaagc gaggcggtca
      301 ccttgtcccc gttggtaaag gggggaaagt tgaggctgcg caccccggtg atgtcgaggt
      361 cggcctggca gacaccccaa aagttggtaa tgccgggggg gctgaagata tcaccccagc
      421 ggtgaagcag gcggtcgctg ccgagctgct ggcaatccgg aatcacaggc attccttatc
      481 agtaaagagt gatctcgagg agatggggat aatactcctc gaattcgata taggtcacgt
      541 agatgatccg gcccccttcg cgcgccaggg ccgggtgttc cttgcccgca tagatgagct
      601 ggggataggg cagatgggcc ggatggctgc agcgcacctg atagagctct gcaggcgtgc
      661 tccagggccc ccagggttcc ggggcggtgc gggcgacgat cttgcccgaa agatcaaggg
      721 agtggaccgc cagatagcac cccaaccagg gattccagga gaccgactgc tcgttgggca
      781 tgccgtcgaa gaggggcacg gcttcttcga tgcgcgacga ccaggccggt cccgtcggct
      841 catcgggtgc gcagccgaaa tccgccgcgc gcggaaggcc gcgctcagat cctggcgccg
      901 cggcgcagag atattcatac cggtccagcc gggtgatctc ttccctgcgc acacgggcga
      961 gatagcagcg ctggatcttg tcctccccct gcagcacccc gtagagatag agccaatcgc
     1021 ccacaggtgt gaggaccgcc gaggcgaaat ggggctgctc cgccggccac cagatgtcgc
     1081 tgccattgtg caggatgcga gtgaaggacc actcccgggc ctccccggcg gcgaggccgg
     1141 aaccgaggat gcggaaattg actggaaaga tcccctcctc cacggtctcg accttgacga
     1201 agaagaggca gattttgccc tcgacctcga tgccgtgcag gcaccagacc cttatccagt
     1261 cggggtcctc ccccctttcg agcgggatga ggggtttgat cccgccgcgg ccgtcgagaa
     1321 tgtagcggta accggcgatg ccggcagggc cgacctcagt ggagctgagc aggccggtgt
     1381 tattgatcat gtgctcgatg ccggccaggc cgctcatatc agcgtggccg accggcttgc
     1441 cgtcagggta ccagaggctt tcgcccggcc ggcgcgcccc gatgagggtg tctccgaaaa
     1501 accagagcag gcctgcctgt gtcgggatgg agtaggcccc gtcctgcccg accatgcggt
     1561 gtgaattctc gcggaactgg gggccgaggt cgcgaatgga tgcaatcctg atcatagcca
     1621 tgaactcatc tctgcttctc gatgctgacg cgcatcaaac cattggccgg caagcagagg
     1681 gtgatcgcat tctgctgcac cgggatgatt cttaaagatc gcccaagcag atcctgttcg
     1741 gtcacagttg cgccgcccag ccagagcgcc gaggtcaggg tcatctcccg gctggcctca
     1801 cgatcgctgt tctgcagcag cagttcataa cggccgttct cctcgccggc cgggcgcagc
     1861 gatatgatct tgatccatgg atccgaggac gcgagatact gctgtatcgg ctcggcgcgg
     1921 gtaaaccagc tccggcgcac gagaggctct gcggcggcct cccagccgaa ggcggcgata
     1981 tcggcctgcc gggcagttgt ggaagccaga cggaagcgga aggtgaggcg gcccgagttc
     2041 tgctcgtttc tgttccacga ttcgggaaag ttgttgacca gattggcgat gacggtgccg
     2101 gcgttgttca gggtgtcgag ggcgaagatg cggcagtcgg gggaggcaag cagcagggtg
     2161 cgctccgccg ccgccgcacc gagcgcccgg cggatggaga agaaggtgtg gccgacgccg
     2221 ctgaagcggt ccccgagggc ggtcaatccg cccagggttt cgcagcggat cccgctgccc
     2281 ggggcgtcca gggcgaaggc gagcccgtat tcctcggttt gttcgcattt gggcagcgcc
     2341 tcgaggtcga tttccacgct gatgtcgatg cgattgaggc cgttccaaag ccggaaggtg
     2401 atggtgcgaa aaatatcccc ggtgtagccc cagcagagtt ctgcgaaggc cggattcctg
     2461 ctgatgcgcg gctcgcctgc ccagccggcg atgggggtga aggattcctg gagctgaaag
     2521 cgcttgcgca ggggcaggaa ggagggcagg gcggcggaat tgagcagggg cctgcccgtg
     2581 gccgcttcga gaatctcgat ccccttgttg gtcgcgctga tgcggtaatg ctcattttcg
     2641 atgatgggcc cggaagccgg tggcagggcg gcatgcgcgc ggactggctt ggccgcgggc
     2701 cggctattat ccggctgtgg gccggcggca gccggggccg gccgggtgac cagctcgtac
     2761 tgccggctgc cgatgccggg gagatccctg gcgatgaagg agagcctttc gccgctgcga
     2821 tggctgggga gtttttcacc gctcgccaga tcgatcaccg cataggagtt ggagtcggtg
     2881 gcgggaaaat cgatttcgac gggtgccgac cgctcccagg agagggtgtt gaagaccatg
     2941 atcccctgcg actcaaagct ctccttcggg gcagtgagcc ggtaaagcag gcgttcgcgc
     3001 atggcgcggc tgcgctgggc ggcattgcgg acatggtcgt cgcgcacggc atcggtatag
     3061 aggttctcct ccttcgagcc caggccatat tcgaggccgc tgccatggcc ggaaaagctg
     3121 agcagatcct cgtagacggc gtcgatctct ccggcgccgg tgcgggtctc ggggtggagg
     3181 gccgaggaga gggcgctgag gacctcgacc ccgggcagct ggttctgcac cccacggtag
     3241 gcggtcatgc ggcgggtttc gccctggtag aggatgtccc agtcggaggt ccagtctccg
     3301 cgccgggtct caagcagggc cggattttcg gcgcagaggg cgcggccgaa atcatcgagg
     3361 gtggcgatga cgaaacgggg ccaggcgtag agattgttcc agcgccgggc gttttcgtac
     3421 tgggcgaggg cgatgccgct gttgtcggaa aaggccccgc tgatgaggat tttgctatag
     3481 ggataatcac ggcgcaggag attattcacc ctgtgccata tccggcaggc ggtcgcctcg
     3541 ggttcgcgtt cgagtccgta gcgcgcccct tcgatatagg cctcggtcag atagagaagc
     3601 agggtatcac tgctcgagcc agcccagtgg aagaccttgg gcaggctttt ctggaattta
     3661 tagtctgcga aaagctcgtt gatgccgttg gccagaaagc gcaccccggc cttgcgcaga
     3721 tactggggca gggcccagga ctggcccggc acgtcgttat aaaccgcagc gaagtagtcg
     3781 aggccgtagc ggctccggta ctcctcggcg aagcggaagc tggcggccat ctccgcctcg
     3841 ctgacccagc cggtgtaggg attggtatag atgggcgaga ccgcgatccg ccccgctttg
     3901 atcagggcaa tgagtcgcgc aaaggcctcc tccgagcgcg cgcggcggta gttttcgagc
     3961 agccacatgg tctcgatggt ccagcggaag ctcgagtccg ccgcacattt ctgcaccgcc
     4021 tgatccaggg tctggagctg gcgttcgatc accaccggct ggggattggt gtagccgata
     4081 tccatgtggg tcaggggcat gaaatagagc tcataatgag gcaggctctt cagctccacc
     4141 cgccggctga aactctcccc tgccatctcg agcacggccg gcaccttgac cggggcctcg
     4201 acctcgggca gatagatctc gcgctgattg accccgaagg tgatatcgtt gagggtgaat
     4261 ttaacatcaa catagggaag ggaaaagaac gccgggattt tggcggtttg gctggaatga
     4321 atctcgagca gatatttttg caccagcccg ccccgcttct ttttgcagaa aaaggtgggg
     4381 gtgagggtga aggcattctg caagccggcg gcctcgatcc gctggctgac gtcggtgagg
     4441 ggggctttgg tgtccggatt gagcaggcgg cagtaaaatc cccattcaaa gcggagcgga
     4501 tcgaaaaagt cgacggcatg attgcggtgg gtattggcgg tgcggatgag gaggcggttg
     4561 ctcccctttt tcagggtcac cggcacggca aagttgtccg ggatggcgtt gcactccttg
     4621 agggactggt agacggtttc gccgttgagg atgatgcggg tgacggtgtt gccgccgaaa
     4681 ctgagcaggg cttcccggct gcgctccgag accatctccg cccaggcgta gacgatcttg
     4741 gcgtaccaga ccttctccgg cgtggcgagg gttttccagc cgtaaaaggg gttcaggtcg
     4801 gtgaagccgt tatccctggt actgagatag agccaggggc tttccccctc cgccagaccg
     4861 gaattccgca cccgcccgcc gctctgtgga gcaacttgca cctcatccag gggggcatca
     4921 tcggagaggt cgccgaagcc gaccgtcctg atctcaaagg gaccgttggt cagccaggcc
     4981 tggaggctcc cgtccacgct cagggggagc gtttccgcgc gctgcgcggc ggcagcgagg
     5041 ggaatgaggg ctaggcagag aatcagtcgt cgcatatctt ttcgcatggt ccttccggtt
     5101 tgacggatgc tgcaaaatta cagccgataa ggcgccccgg cctcgcggat tcactggatc
     5161 agcatcatct ttctggtttc acgccggcca taagtttcga gggtatagaa aaagaggccg
     5221 ctgccgaggt tgttgggctg ccagcgcgtg gtatagcggc cgggtgcaaa actccgggat
     5281 tcgagcaggg tttcgaccag ctggccgcgg gcgttgtgga ttctcaacgt cacctccgtc
     5341 cgctcgttga tctcgaaggc gatctcggtg gccggattga agggattggg atagttttgc
     5401 agcagggcaa aggccgccgg cggcggcacc ggctccgcca ccccgctgcc gatgccggcg
     5461 gtcgccagct tgataaagcg ggggtggtag atatcggcaa cgcgcaggtt ctcgccgaaa
     5521 tcgagggtat tgacgttgta ggtgataagc agggagtcgg catccgatag cgaggggtgg
     5581 gccttggcgt tataaatgaa aacattgtcc gagatctcga cctcgggggc cttgtagatc
     5641 tcccggtaca tctcgaaggg tccgacgggc gtgtcgccga agcggacggc gaccgcggag
     5701 ccgatctggc agacaaggat atagcggccg ttcagttcgg agaggctgaa ctcctgcgag
     5761 atgcggttgg tgatggaggc gctggcagta tggtcgctgt tccagccgct gccgtcccag
     5821 aatcgccagg cggtgaaatc gagaagctcc tccggccgca cccgaccggc gaccaggctc
     5881 ttgccggaac tgtcatcgcg ggggccgtag atgtagatat agccgtcggc cctgctgttg
     5941 ccggaggctg ccgagttggg caaaatcgcc tggccgagga ccgcagtcag ctcgccatcc
     6001 ttcatgaaga agggcagatc gcgctgctgt actccggccg gataatggtt ttcatcgagg
     6061 gtgaaggaga gcagcaccac cccggtgagt ttccagtcaa aggcgccgct gccggtggtg
     6121 tgcaggcgca gggcgaagag atggatgagg gagtcgatct tgaccccgtc catcaaccag
     6181 taaaaatcgt tcgcgaccgc cgtgggggtg gcaggtgtga aaaccgcgcg cggcgaggcg
     6241 ccgctctggt cccacatgaa ggcgatgcgg ccgggatcgg gccggctgcc ctgcaggtgg
     6301 gcgagggtgt tgttgacgat ggtggagttg acccgccggc cggaggcgtc gacctggccg
     6361 atgaaggtgt cgctgaagat gatgagatgg tcctggccgt gcggctgctc actgccgtca
     6421 agcggaatcg aatagatgcc gtcggcgccg gtccagcctg agctgcgtgt caccagctgc
     6481 tcccacgcct ccgacctccg gacctcgtac tcgcctgggc aggaggcccc ggccgggagg
     6541 gcgataggat tgggatagga gaagggagcc cagccgtcgt tgccggagca gagcagattt
     6601 gtggtcgctt tggtgaccag atagccgagt ttgaagcagc ctgggctggc gccggtctgc
     6661 agccgcacct cgatatcgat ctgaatagcg gcggcgggag cgtagccctg gtcggaaaga
     6721 aggccgaccc atttcatctc cccctcaaac tctgcaggcg ggtagcaggc ggcgagggag
     6781 tcggtccagg cggtgctgta ttcgagatcg ccgtcaccca ccgtggacga atagacggcc
     6841 tccagcacgc gccagtccga gggcaggagc agaccgagca cccccttgtg gacgttggca
     6901 tccgcgccgt cctggacggt gaggcgcacc gtgatctcgc tgttgagctc cgccgactgc
     6961 ggctgagtga tctgggttat cttgcagctg ctgagcagaa gcggcataag caggaccaat
     7021 cctctgataa ttttatcttt tttcaaaatg tgaatcctgc cgtaaagcgg tgaatgtcat
     7081 caaaaacccg ttcggactgg acaaaggagt aatcgaggcg gaggttgact ccagccatgg
     7141 caggggtgac cagtccgaag ccgcaggcca gtttgccctc gtcggtgttg aagcggtagc
     7201 cggcgcgcaa gaagagaagc tgatggagct gcagctcggt gccgatgttg acgatttcga
     7261 cattgtcggt gggatggacc agttcggccc cgagggtcca ggccatttcc tccgatttgt
     7321 agagttcgtc ggtggcgttg atgcggaaaa gaagcggagt ccggtactcc tggtcgacca
     7381 gttccatgtc gggtccgaaa tgctggagcg agaaggcgag gcgcaggctg cggaagccgg
     7441 tgtaatagag ggcgccgatg tcgaagagca gattgttgat gctgtaatcg tcgagttttt
     7501 cacgcacgta cttgacctgc ccgccgatgg agagtttatc ggtgaacatg cgggccgcgg
     7561 agagtccgac catgaggtcc cccgcgctca ccatgcgccc ggtcccctcc gggctggtga
     7621 cggtggtctc ctcaaagtcg tcgatggcga aggagagggc gctgagggcg acggtgaagg
     7681 tctttaaggg aatcgccacc gcagcggcca tctggcggct gttcaccagc caggtattgt
     7741 gggagaagga ggcctgatag cgggtcgcgt aggccagacc cgccgggttc caaaagaccg
     7801 cgttgacatc cttgacgtcg ccggcgaccg cttcacccat ggcgctctcg cgcgcaccga
     7861 ccccgatttt gagaaactgc aggccgcccg agccggtctt ggcaaaatca aagccgtaat
     7921 cgtattgggc caggagcaag cacggcagca gcagtaaggc aaggagtatt tttgctttca
     7981 tagtcgtctt ctactttatg ataagtaaag tgcccttggc atggcccacg ggggattcga
     8041 ccgtccagaa atagatcccg ggggcagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnccc ttggcatggc ccacggggga ttcgaccgtc cagaaataga tcccgggggc
     8221 aggccgctga cgcgcatcgg tgagttggtc ccaggtctcc tcaccggaga agggactgtt
     8281 gtgctccatg gttttgatca gttcgccgtt gctggtgtag atgcggatgg tgcactcggc
     8341 cggcaggttg gtccagacga tcgagttttc cgccccgctg accggaaagc cggaggtgat
     8401 gcgaaaaggg ttgggaaaga cggtgacgct gagggcgtcg gcggccgggc tgcggatggc
     8461 atagaccggc ataaagttgc gattggtcag ggcgctctcg ttgccctcag catcgacggc
     8521 ggtgaccgca tagaaataac cagcgccgag gctgatggtg ttgtcctcaa agatccattt
     8581 gttcttcatg gtgtcgaagt agcgcgtcac atcagtcgcc cggccgggcc ggatcgcctt
     8641 gagcaggtca taaggcccga tgaaggcgag gctgctgcgg tagacgcggt attcgagaaa
     8701 atcgccgctg ccggtgaggg gatcgaccca gctctggtcg acggggtccc agttgagggt
     8761 gatcgactgg ttctcaggca ccgggatgat ctgcagcgcc ggcgaaggcg gcggcagctt
     8821 cttcatggca tagccggcgg ctaaaagctt ttgcgccgcg aagatggtct cctgcagcat
     8881 ctcctcccca tcctccagca tgggctgggc atccagcccc ttgagggcgt cgcgcaggga
     8941 gatgcccgcc accgcctcga cgaggacgat gcggaccgat tcatccttgg ccagatcgta
     9001 ggggccgagg ctcatcagca tcatcggcgt gaggtgcgcc tcctcatccg cctgcaggct
     9061 tttgtcctgc ccgctgagga tcttgtagag atcggcagcg gcgctgctgg tcagggagaa
     9121 gtagcgttcg ttgttgagga gctgggccca gagcacgact gcgggctgga agcggctgcc
     9181 cttggccggc gtggcctgga gcagggcgta gccggcatac cccgtagtcc ggagttcgct
     9241 gcggtcgtcc cagtagttgc cgacgtcggt cggcagggta tagttggggg tgtcatccca
     9301 ggcgtagagc agccggtagt cgtcgtcgta gcggaccagt tcgtcggtgc ggttgaaatc
     9361 atccccccag ccattgtgga cgacgaaatc ctggtagctg ggccggatca gatagggaaa
     9421 gccgagaaag agatcggtga cccggcccgc attgcggttg atgaactcgt actccaggag
     9481 gatgaaattg tcattgcccg ggaagctcca ggcccggctg gtgcgtttga cctcgatgtt
     9541 atgggtcgtc gtcagccgca tggagatgac ctgctcagcc tcggccggat cgtaaccggg
     9601 ttccccgata aagttgttcc gcacctcgag gggataaaag gtgccctggt cgacataggt
     9661 gaagggaccg ttctcggtgt aaaagaggct cccgtcctcg tggtagccgc cgatccagag
     9721 tccggctccg gccatgtagg agcgctggtc gtccttgacc atggtttccg gtccgcccgg
     9781 ccagtccata ctcggatagt actggaagcg gttgacgggg tcgggggcgc cgatggtgcc
     9841 gtcgtccttc atggtctccc agaggacccc gcggttgtgg atgcggaaat ccgccttgcg
     9901 cgcctgggca aagagcgaca gggggagcag cataaagcaa agcagtttgt atttcatggc
     9961 aggccctttt cggttagcct tttcattact tgtacaggat aatccttcca cagtggcgca
    10021 gagaaagcat aactctcacc aagccaccaa gccacaaaga aataataaga aagcttctct
    10081 ttgtgtcttt gtgcctccgt gagagatagt tttcattttt tctctgcgcg agattattaa
    10141 aaagaatatt tcacccccat ataaatcctg cgggtttcga aatagttctg ccagccgatg
    10201 tcatacccat ccgcatccac agtcgatacc gtcccctttt ctataaaatt cttggtggcc
    10261 tcgctgtcga agagggcgtc gccataggag cgcagattct tgcggttgaa gaggttggtg
    10321 acgttcatgt agaacgaggt cgtgtggccg ccccaaaggg gaaactgctt ttcgagcttg
    10381 aggtcgaagg tgtgaatggc cgggaagcgg tagttgttat aggtatccgg cgaatcggtc
    10441 gcctgcagat aggtgaagga ctgcccgctg taatagttga agagcaggct catggtgctc
    10501 tccctgaaga cgcggtcgat caggcgggcg ctgctgctcg cggggtagcg gagatagctg
    10561 ttgacgcgca ggacgtgggg ccgtgagaag ctcttctcga tcgggatacg cttgttgaca
    10621 tcgctgtccc aggtatagtc gatattgccc tccccgtcat agcggatgac gcccggggtg
    10681 gcgcggcctt ccgtggcgcg gctgaagctg tagttgacgt cgaaaaagag caccttgctg
    10741 aagagggaat ggagggccgc ctcgatgccg cgcgagtcac cgtaatctcc cgtcagataa
    10801 gtctggtagg aggcgttggg cgtggtgact ccgctgaagg gatcccagcc gtagatgtgg
    10861 tggtcgaaga ggccgatgcg ctcctgggtc tgatcaaaga catccttgta ataggcgacc
    10921 aggtcgagga ccgcaatacc ctcgaagctc tgcttgaagc cggcttcaaa ctggatggtc
    10981 ttctgcggct tgaggggctg gttggtgaat cccaccaccc gttcggcgtc gccgtcgttg
    11041 gaggggatat ggcgctcctc cctgccctcg gccgcggcgg cagtgcgctc ggcgaggata
    11101 ttcttgacga tgtactccgg ccggaaataa ccgatctcga acgactggtc gaggggcggc
    11161 atctggtaat agtggccgta gttgaaatgg agcagtgagt tgtcgctgat cgggaaagag
    11221 acgccgaagc gggggctgat catatgccag gctttggcgg gtttgcgcgc cttgtccagg
    11281 acgttgtcga aggcgtattt gacatggccg ttggcatcga cctggtcctt gtccggatcg
    11341 agcgccggat cgaaggcggg atcgatggcg aggttataga gggtggtcgg ttcgaaccag
    11401 ttgatgttgg gatcgtaata gtcgtagcgg acgccgacgt tgaggatcat gctctcaaat
    11461 tccatcttgt cctgcagaaa gagggcggcc tggaggggat gatagcgctc catgaggccg
    11521 cggttcagcc agagcgactt gtcgcggctg aatgagggga tgcggtaggt ctcccgctcg
    11581 tcataggtgt tgtagcggag ttcaaagccg ctcttgagca gattattggc atcgaactgc
    11641 cagttgacat tgcccgtgag ggagagatcg ccggtgagaa tgtcgttgta gaggacaggt
    11701 gaatagttat cgaagggctc ggactcatag ccgtcgaagc ggtggaaccc ccacagggtc
    11761 ggcttctggc tggccccgac cagtgaaaag tcgctgggct tttccttgaa atgcgaccag
    11821 taggtggcca gtttgagttc gtaaaaaagg cgctcgttga ggaactgggt gaacccatag
    11881 ttgagcagcc aggtatcggt attgagcagg gagccccagc cgcggtagta tttggcctgg
    11941 ttggtgaaat cgccgttctc ctggagggtg gaatgggcgt cggtgttgta aaaacccgtc
    12001 agcttcattt tgatgttttt gggattccag gcgatgttga acatgccatt gccggcgtcg
    12061 cgcgaatcgc gcgggtgcgg cagtgtgtag gcctgctttc gcaggttaag ggcggagaaa
    12121 aatgtgaccg gagccccctt gaggcggaag aggggcccgt ccaggctcag ggcggcggtg
    12181 taatcgggga tcttgcggta atcatagacc tgggcctggc gtgtcgtggt ccaccactcc
    12241 ggattgaggc tgccatccgg cagggtgttg tcgcggaact ccttgtgact ggcgcggtca
    12301 taaaggtagg gaccgaagtg gtgctggccg gcgaagccgt agctgaaatc gacagagccg
    12361 tggagggcgt ctcccccttc acgggtgacg atgctgacca cgccggactg ggcatcgctg
    12421 tactcggcgg taaagccgcc ggtcatcacc tgcacctcct tgatggcctg catggggacg
    12481 ttggtgaagg agccgcccca gtcagcgcgg cccaggagca ggttgctcac gcgcgctccg
    12541 tcgatgaacc acttgacctc gtcctggctg ccgccgcgca ccgaataacg ggtctcgccc
    12601 ttgcccgccg aaccgagccc gccagcgacc gggatgggat cgagaaagac cccggcctgg
    12661 gccatcaacc cctgcttggt gtcgcgcatc cccatgcgcg acaccaagca ggggatgcgc
    12721 gcaaactcgg cgatgccgat gttctggatg ctggcggtga cgtc
//
LOCUS       DAOPWU010000066        12754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5013_length_12754_cov_5.198835, whole genome shotgun sequence.
ACCESSION   DAOPWU010000066 DAOPWU010000000
VERSION     DAOPWU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12754
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5013_length_12754_cov_5.198835"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKM23_05275"
     CDS             <1..548
                     /locus_tag="PKM23_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNY90919.1"
                     /translation="QSTYELYRRGGSLEKVCSGLAALRAARRARGGRTPRLYLQFVVM
                     RHNEHELPLLRAEAAKWGADQVLVKSPYLRSVEGAEEILPAEERYRRYRCIEGHWLRK
                     EKAARPCPRLWYSSVIHWDGKVVPCCFDKDGSHLLGHASEPFKKLFHGEAYRQFRRRQ
                     YSAHAPQICQNCTDGLKIYPA"
     gene            523..1491
                     /locus_tag="PKM23_05280"
     CDS             523..1491
                     /locus_tag="PKM23_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNY90920.1"
                     /translation="MASKYTRHKRTLLIFKAVIAIIVLTMLVRHISIGELKTALLEAC
                     IVWIIPAVLLLALNLFFQFKRWEMLVRQVQPRAPKRQILSSLLSGITLGFITPGRVGE
                     FGRAFFIDGTDWPKLLGLTLLDKSYAFMTLLFLGLVGMLPYLRREVELLVWMPLIIIA
                     LAFFALLFLLLLHPPFLAGILKKFRKLWEHRRKVAQIVSSLEQVPPRLAVRISLLALG
                     QVFTYLLQFYLLIRAFSPLPLLRGIQALMATLWVKTMLPISIGDLGVRESAAIYFLGE
                     LGVAQAAAFDGSLLLFFINVLLPALAGLILLLRRDAAARKAHSGRI"
     gene            1500..2657
                     /locus_tag="PKM23_05285"
     CDS             1500..2657
                     /locus_tag="PKM23_05285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90921.1"
                     /translation="MIPALLYLLLAIAYSLFIVWILQGLRRIRMPWSGEIVKKVSVII
                     ATRDEEQNIPFSLGTLLKQSHPRTHLEIIVVDDHSSDRTRSVAEGIAAKFPMIRVLAA
                     PSFSSSIAPKKAALAAGIGAASGEIILTLDADCAAPSGWVEKMSAPFAGDITAAASWV
                     LIPEEKSLPTQIEFLDALALQLIGAAAIGWEKPFLANGANLAFRRRTFQKIGGYEGFA
                     YMGSGDDDLLLQKLHADGDGRILFNTDTDSAVTTLPCKSWRDFFRQRIRWSSKAAFYP
                     TWIKAIEGAAFVHYVTLLLGIPLALLLSFPLWVPLLALLCKMTCDYLLLHKGVRMVRR
                     SWSWPGFFGASLLHIAYIPVIGLIGQFGRFEWKGRTYRQGRLEKNAAMKRV"
     gene            2754..4328
                     /locus_tag="PKM23_05290"
     CDS             2754..4328
                     /locus_tag="PKM23_05290"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNY90922.1"
                     /translation="MKIKTALLLTGLAAFASSAQSVHVPLDYWGYGFLERMEAKGLFH
                     SLALLDRPLNRNQVAQLIGEIEDKAAVKPSSLSATDLRLLEQLKGDFADELSSQGGRS
                     LPARRELHAARWSEGPNQACIDLYSRLSIVSNRGRSYDSPELLAEGTLGAILRGQLGA
                     TLGFYADARNTLTRGEKTAGDRDENFDVSKGSPVVISGPNLFRDRALAYFVWEKPWLR
                     IQAGRDEIDWGPGSHSGLALTRNMPPADMIRLASRFRKFAFSSAHIWLRTPLGAKYLA
                     AHRLDFLLLSGLQIGAGETVVYGKRDIEMSYLNPLMLYHVAEHHLGDRDNNTLFLNLS
                     CTLLRGTRFYAEYFIDDMTSTESLTRYFGNKFAFMTGVLWSDPLGARNLDLRFEYSRV
                     EPFVYAHHDSLNIYTHYDKTIGHWLGPNSDSAWLLAARQFGRDLRLEASLERIRKGEG
                     AADTRARPAQGTVKQFLGGVVEKRYLAGLRLQEQVRRDLFVSVAYTYAHIRNAGRVEG
                     LSGDDHLARFEIAYNY"
     gene            4338..5480
                     /locus_tag="PKM23_05295"
     CDS             4338..5480
                     /locus_tag="PKM23_05295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90923.1"
                     /translation="MTALLLLLTLLYMVALLLISRGLSRLQPPAAPATRPFISVIIAA
                     RNEADHVTPLLEALARQDYPREQHEIIIVDDASSDGTAETIEQWLRANPHCPIRLLHS
                     EGRDQVISTKKHALAQGINAARGEILLFTDADCQPPTTWISSLCARFGDQVGMVIGYS
                     PYEIPAPHTLGQRFLALESLSLAALAAGTTGWGRPATCTGRNLGYRKSVWLQVNGFEP
                     IARFVSGDDDLFLKQVLDRTSWRIAYALDPGAIVPTRLLPSGKAFFRQRLRHASKGFY
                     YGWKMSSILALAWLFNLLLLVRAISSLCGPAITPWAWIAWAGKAAAELLLLIRFAGPM
                     QRMHTLTVFPQAEAAHVLYVVLFGALGPLTRVRWKETTAHTHTLKR"
     gene            5490..6644
                     /locus_tag="PKM23_05300"
     CDS             5490..6644
                     /locus_tag="PKM23_05300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90924.1"
                     /translation="MSSLTILAWLTLLLAAAYLVTILLFLIGLRRPASPGFTGLPVVT
                     VVVAARNEEKHIGSLLDDLTRQDYPDHLYEIIIADDGSSDRTAGIVRERMAGSPFIRL
                     LSIEECPAGFSPKKYALQRAVSQARGEIILATDADCRLGPQWASTMVKYFLPEVGFVI
                     GFSQYGRRGEKQNLIERLQAMDFIPLMGVAEGSCNLGLPLSASGQNLAYRREAFHAVG
                     GYSRVAHRVSGDDVLLLQLIRSHTDYRVVFASHPGAFASSAPQPTLADLINQRKRWAS
                     NGSFQLFLNIPFFAYLIMVYLYTAALFVGVPLSLLLGTHAGLFALCLASKIGGEWAIS
                     LVSAHRYGRTDLLRWFPLWFIVQIPYIVWVGMLGSFGNFKWKDRKHGAAL"
     gene            6641..7699
                     /locus_tag="PKM23_05305"
     CDS             6641..7699
                     /locus_tag="PKM23_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNY90925.1"
                     /translation="MILPVLEKAPAALSHAWHKRPLNSSTEVRLTRLCTQRCRQCQVY
                     ERTTEPASMDIHMFRHIARELSRYGAYIGFISGGEATMVHDLPQILLESRKTFPVATT
                     LVTGLYNKSELIRDIAQICLDNQINIQTSLDGFGETGDLLRGAKDFSATVLRHMEMIS
                     AMRSSSRSLLYCNIVLSRLNLPQVPELIAAARAIGWKATIGLYHHLTETTREDDNLSV
                     RPGEDLDKLVAFLDNNPDILNLNAWIRGIAPFISRGETTPCPFVASRFLSSRTTIMEN
                     GDLHLCWGGPVGNLFTHSMKEIFSGDAYRQRLEEYQNCRGCWTTCYTQRYLLVHPRSL
                     GEALNNSRKIVRMQRRRG"
     gene            7696..8526
                     /locus_tag="PKM23_05310"
     CDS             7696..8526
                     /locus_tag="PKM23_05310"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNY90926.1"
                     /translation="MKKRLFQATFPALRRLLAGSALLLGLIQPGSPNPLCAQENSTAG
                     PLVFDETAEYSLAFKGLGAAQASIRFFPGTPQRIEARIDTRGLTSLIFSVHNTYQSHI
                     DPSSGLPIVVEKNIAQSNIRQQLSIRYDRPDNTAESSTPASWSILPGAMDLFTMLFQL
                     RRMSLPPGAKVAFPLDIESQAWLASGQVEKGGEMESPCGRLETRRITLLFSRMDQGSP
                     RAWKTDLLTNRIARPDGMLTLLLGPPPEQIPLLIQFGPPGQQVSMRMKNYTRGNRSCP
                     "
     gene            8523..9656
                     /locus_tag="PKM23_05315"
     CDS             8523..9656
                     /locus_tag="PKM23_05315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY90927.1"
                     /translation="MIILFTGGLYALLIALLYRHLRCLFAEERRPLQDEPMVSVIIAI
                     RNEAPHLIDCLESLAALDYPRDRLEIILIDDGSSDGSAELLHDFHDRHPWVQAIRLEG
                     DAKQLPGKAGAVYRGITGSRGEIIAMTDADCRVPPGWIRHLLAAFDDGVGLVGGFTFL
                     ESTARKRTCFEAVQALDWLFLLGIAAAAIKMGKPLTWMGNNMAFRRRAYEDVGGYPAL
                     GHNLIEDFALLNAISRETEWKVRITPSNQAAVLSLPVGSLSALYNQRRRWALGIRQVR
                     PFGKLLLVTSFLAHLGATAGLICCRPAALATLVLLLAGDLAVCRKMSSTTANTPLLRH
                     LPAFELLYFSYTLFLPLLILFDRRIRWKDQNFPAHAKKNRGTA"
     gene            9670..10722
                     /locus_tag="PKM23_05320"
     CDS             9670..10722
                     /locus_tag="PKM23_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY90928.1"
                     /translation="MSWIPPLLLHYYITERCNSRCSFCDIWRQPAGPDALLEDVERNL
                     HDAARLGLRFVDFTGGEPLLHADLPRMLRRARSEGLRTTLTTNAMLYPDRAAELVGNV
                     DFLHFSLDASTAADHDRLRGRPLFDRVMASIDLARQLGEKPDLLFTALPDNLHHLPGL
                     AEFCRRLGLVLIVNPVFSHREKRELNPEDLAFIDQFARRPFVYVNRAFHLLRKRGGNS
                     IVQPRCRVVDSVVVISPQNQLILPCYHHEQARISLVEGLAACWHSAAVNELRAQQGRW
                     PACAGCTLNCYFDPSFACRIDALFMHSMAAKIKYARDKYVRAPLMRTLSRTDPRPAAG
                     IMASLSKPDDSPAEAR"
     gene            10719..12356
                     /locus_tag="PKM23_05325"
     CDS             10719..12356
                     /locus_tag="PKM23_05325"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNY90929.1"
                     /translation="MIASGFSAPPMPALLLIGFFLYVETHFRIGRLPSRYFRRQPEIL
                     FDIPHRVAPGADLPLLLIIKDADRYPVELNDISVDIKQEGKILSTTRLPLGDERVDQP
                     LWWRRFPLIVRGFEGEVTVEPMLTGRCSGRALEVRVDNYPTLSHAPLNCRLDRDQLPG
                     AERWLYGDLHYHSHLTSDQVEFGAPPAAAAAMAAAMGLDFFAVTDHSYDLDDRWDDYL
                     HNDEELAKWKWLQDFVREWNGGGRHPLIIPGEELSAGNSRGGNVHFLLYNEPSFFPGW
                     GDSAEKWLQTKPQWTIRKVLDQLSPQALAFAGHAESAPPRLQRLFLGRDHWRAADYEH
                     PRLDGVQMWNRLRDPAFSRGLAAWQKLLLAGRRPVLIGGNDAHGDFGRWRQISLPFVS
                     LKEKEEHLFGQVRTGIIRPAGTATLPDLLHSLRAGRCLVTSGPSLWLELITETGPPCT
                     PGDTCTGAPRKLHIAADSTPAFGPLARVTLCIGDLQRKKETRRPLPIGPAGSMNLTLD
                     EPARELPGQGYLRLEVSSDREGTPFHCFSNPIYLAWR"
     gene            complement(12374..>12754)
                     /locus_tag="PKM23_05330"
     CDS             complement(12374..>12754)
                     /locus_tag="PKM23_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNY90930.1"
                     /translation="GSLAPVSLLNLDTNERRQVHRHFDRSLEYATKTYKLAENEFDLR
                     VYPVGNLRRLAEKSAAEAIQTGKKVALPPMSSYERFVIHEALKTNESVKSASHGEGAE
                     RHVEVEPELYGRSLKKMIKKIKLI"
BASE COUNT         2286 a         4663 c         3328 g         2477 t
ORIGIN      
        1 cccagtccac ctatgagctc tatcgccgcg gaggcagcct ggaaaaggtg tgcagcggcc
       61 tcgccgcact ccgggcagcc cggcgcgccc ggggcgggcg caccccccgc ctttacctcc
      121 agttcgtcgt catgcgccac aacgagcatg aactcccgct cctgcgcgcc gaggccgcaa
      181 aatggggcgc cgaccaggtg ctggtcaaat ccccctatct ccgctcggtc gaaggagctg
      241 aggagatcct ccccgcagag gaacgttacc ggcgttaccg ctgcatcgaa gggcactggc
      301 tccgcaagga aaaagctgcg cgtccctgcc cccggctctg gtactcctcc gtgattcact
      361 gggatggaaa agtcgttccc tgctgttttg ataaagacgg ctctcatctc ctcggacacg
      421 cctccgagcc gttcaaaaag ctctttcacg gcgaagctta ccggcagttc cgccgccggc
      481 agtattccgc ccatgctccg caaatctgtc agaactgcac cgatggcctc aaaatatacc
      541 cggcataagc gcaccctgct catcttcaag gcggtaatcg ccatcatcgt cctgaccatg
      601 ctggtacggc atatcagcat cggggagctg aaaaccgccc tcctcgaagc ctgcatcgtc
      661 tggatcatcc cggccgtgct gctgctggca ctgaacctct tcttccagtt caaacgctgg
      721 gagatgctcg tccgccaggt ccagccccgg gcgccgaaac ggcagatcct ctcctccctg
      781 ctttcgggca tcaccctggg cttcatcacc cccggcaggg tgggagaatt cggccgggcc
      841 tttttcatcg acggcaccga ttggcccaaa ctgctgggcc taaccctgct cgacaagagc
      901 tacgccttta tgacgctcct cttcctcggc ctcgtcggca tgctccccta cctcaggaga
      961 gaggtcgagc tgctggtctg gatgcccttg atcatcatcg ccctcgcctt tttcgccctg
     1021 ctcttccttc tgctcctcca tccgcccttt ctggccggaa tcctgaaaaa gttcagaaag
     1081 ctctgggaac accgccgtaa agtcgcccag atcgtctcca gcctcgaaca ggtgccgccc
     1141 cggctcgccg tccgaatctc cctgctcgcc ctggggcagg tcttcaccta tctcctccag
     1201 ttctatctcc tcatccgcgc cttttctccc ctgcccctcc ttagaggcat acaggccctg
     1261 atggccaccc tctgggtgaa gaccatgctc cccatctcca tcggtgacct cggtgtgcgc
     1321 gagagcgctg ccatctattt tctcggcgaa ctcggggtcg cccaggccgc cgcctttgac
     1381 ggctcccttc tgctcttttt tatcaacgtg ctgctgcccg cactcgccgg gctgatcctg
     1441 ctgctgcgcc gggacgccgc cgcccgcaag gctcactctg gaagaatctg aggtctgtca
     1501 tgatccccgc cctcctttac cttcttctcg ccatcgccta cagcctcttc atcgtctgga
     1561 ttctgcaggg gttgcgccgg atcagaatgc cctggtccgg agagattgta aaaaaagtct
     1621 ccgtgatcat cgccacccgc gatgaggagc agaacatccc tttctctctg ggcacactgc
     1681 tgaaacagag ccacccgcgc acgcatctgg agatcatcgt cgtggacgac cactccagtg
     1741 accggacgcg gagcgtggcc gagggcatcg ccgccaaatt tcccatgatc agggtcctgg
     1801 ccgccccttc cttcagcagt tccattgccc cgaaaaaagc ggccctcgct gcgggcatcg
     1861 gagccgcctc gggcgagatc atcctcaccc ttgacgccga ttgcgctgcc ccctccggtt
     1921 gggtggaaaa aatgagcgcc ccctttgcgg gcgacatcac ggccgccgct tcgtgggttc
     1981 tgatccccga agagaaaagc ctccccaccc agatcgaatt cctcgacgcc ctggccctcc
     2041 agctcatcgg cgccgccgcc atcggctggg aaaaaccctt cctcgccaat ggggccaatc
     2101 tcgccttccg ccgccgcacc ttccagaaaa tcggcggcta tgagggattt gcctacatgg
     2161 gttccggaga cgacgacctt ttgctgcaaa aactgcacgc cgacggggac ggcaggatcc
     2221 tcttcaatac cgacaccgat agcgcagtca ccaccctgcc ctgcaagtca tggcgcgatt
     2281 tcttcaggca gcgcatccgc tggagctcca aggccgcctt ctatcccacc tggataaaag
     2341 ccatcgaggg cgccgccttt gttcactatg tcaccctcct gctcggcatc cccctcgccc
     2401 tgctgctctc ctttcccctc tgggtgcctc ttctcgccct gctgtgcaag atgacctgcg
     2461 actatctcct cctgcacaag ggtgtacgca tggtgcgccg cagctggagt tggccaggat
     2521 ttttcggcgc ctccctgctc cacatcgcct acatcccggt tatcggcctc atcggtcagt
     2581 tcggccgttt tgaatggaaa ggacgcacct atcgccaggg ccggcttgag aagaacgcgg
     2641 cgatgaaaag ggtttgaatt catccgcatt attgatatat tctacgacag acgccgtccg
     2701 cccgccagaa cggacgaccc accggtccgc aagtatgcgt gagaatatgc ccgatgaaaa
     2761 ttaagaccgc actgctgtta accggactcg ctgcctttgc gtcctcggca cagagcgtgc
     2821 atgtgccgct cgattactgg ggttatggct ttctcgaacg catggaagcc aaggggctct
     2881 tccacagcct cgccctgctc gaccggcctc tcaaccgcaa ccaggtggcg cagctcatcg
     2941 gcgagatcga agataaagcg gcagtcaagc cttcctcatt gagcgccacc gacctcaggc
     3001 tgctcgaaca gctcaagggc gactttgccg acgaactttc atcacagggc ggccgctcgc
     3061 tgcccgcgcg ccgggaactg catgccgcgc gctggtcaga aggcccgaac caggcctgca
     3121 ttgatctcta cagccgcctc agcatcgtct ccaaccgcgg cagaagctac gactcgcctg
     3181 aactccttgc cgaaggcact ctcggcgcga tcttgcgcgg acagctcggc gccacccttg
     3241 gcttttacgc cgacgcccgc aataccctca cccgcggcga gaaaaccgcc ggcgaccggg
     3301 acgagaattt tgacgtctcc aagggctcgc cggtcgtcat ctccggcccc aatctcttcc
     3361 gcgaccgcgc cctcgcctat ttcgtctggg aaaaaccctg gctgcgcatt caggccggcc
     3421 gggatgaaat cgactgggga cccggatccc acagcggcct cgccctcacc cgcaacatgc
     3481 cgccggccga tatgatccgt ctggccagcc gcttccgcaa attcgccttc agcagcgccc
     3541 atatctggct ccgcaccccc ctcggcgcca aatatctcgc cgcccaccgg ctcgatttcc
     3601 tcctcctgtc cggtctccag atcggcgctg gcgagaccgt ggtctatggc aaacgcgaca
     3661 ttgaaatgtc ctatctcaat cccctcatgc tctaccatgt ggcggaacac cacctcggcg
     3721 accgcgacaa caataccctg ttcctcaacc tcagctgcac cttgctgcgt ggcacgcgct
     3781 tctacgccga gtactttatc gacgacatga cctcgactga aagcctcacc cgctacttcg
     3841 gcaacaaatt cgcctttatg accggggtgc tctggagcga tcccctcgga gcccggaacc
     3901 tcgacctccg tttcgaatac agccgcgtcg agccctttgt ctacgcccat cacgattcct
     3961 tgaatattta tacccattac gataaaacca ttggccattg gctcgggccc aactcggatt
     4021 cggcctggct gctggcagcc cgccagttcg gtcgtgatct gcggctggag gccagcctcg
     4081 agcgcatccg caagggggag ggcgcagctg acacccgggc ccggcccgcg cagggcactg
     4141 tcaagcagtt cctcggcgga gtggtcgaaa agcgctacct ggccggcctg cgcctccagg
     4201 agcaggtccg ccgcgacctc ttcgtctcgg tcgcctatac ctacgcccac atccgcaacg
     4261 ccggccgcgt cgagggcctc tccggagacg accacctcgc ccgtttcgaa atcgcctata
     4321 actattaacg gacgaagatg accgccctcc tgctcctgct cacgcttctc tacatggtcg
     4381 ccctgctcct catctcccgc ggcctcagcc ggctccagcc cccggcggcc ccggcgacca
     4441 gaccctttat ctcggtcatc atcgccgccc gcaacgaggc cgaccatgtc accccccttc
     4501 tggaggccct cgcccgccag gattatccgc gcgaacagca cgaaatcatc atcgtcgatg
     4561 acgcctccag cgacggcacc gcggagacta ttgagcagtg gctccgggcg aacccgcact
     4621 gccccatccg cctgctgcac agcgagggac gcgaccaggt gatctctacc aagaaacatg
     4681 ccctcgcaca gggcatcaac gccgcccggg gcgaaatcct cctcttcacc gacgccgact
     4741 gccagccccc caccacctgg atctcctccc tgtgcgcccg cttcggcgat caggtcggca
     4801 tggtgatcgg ctactctccc tatgagatcc ccgcgccgca taccctgggc cagcgcttcc
     4861 tcgccctcga gtcgctctcg ctggcggcac tcgccgccgg caccaccggc tggggacgac
     4921 ctgccacctg caccggccgc aacctcggct acaggaagag cgtctggctc caggtcaacg
     4981 gctttgaacc catcgcccgc tttgtctccg gcgacgacga tctctttctc aaacaggtgc
     5041 ttgaccggac cagctggcgg atcgcctacg ccctcgatcc gggcgcgatc gtgcccaccc
     5101 gcctcctccc cagcggcaag gccttttttc gacagcgcct gcgccacgcc tccaagggat
     5161 tctattacgg ctggaaaatg agcagcatcc tcgccctggc ctggctcttc aacctgctcc
     5221 tgctcgtcag ggccatctcc agcctctgcg gccccgcgat cacgccctgg gcctggatcg
     5281 cctgggccgg caaggccgcc gcagagctgc tcctcctcat ccgcttcgcc gggcccatgc
     5341 agcgcatgca taccctgacc gtcttcccgc aagctgaggc ggcgcatgta ctctatgtgg
     5401 tcctcttcgg ggcgctcggc ccgctcaccc gggtgcgctg gaaagaaacc accgcacaca
     5461 cccacaccct gaaaagatag agaatctgca tgtcctccct gaccattctg gcatggctga
     5521 ccctcctgct ggctgcagcc tacctcgtga ctatcctcct ctttctcatc gggctgcgca
     5581 ggcccgcctc cccgggcttc accgggctcc cggtagtcac cgtcgtcgtc gccgcacgca
     5641 atgaggaaaa acatatcggc tctctgctcg acgacctcac acgccaggat tacccggacc
     5701 acctctacga aattattatt gcggatgacg gctcgagcga ccgtaccgcc gggattgtcc
     5761 gcgagcgcat ggccggatcc cccttcatcc gcctgctctc catcgaggag tgcccggccg
     5821 gcttcagccc caaaaagtac gccctgcaga gggccgtcag ccaggcaagg ggcgaaatca
     5881 tcctggccac cgacgcagac tgccgcctcg gcccgcaatg ggctagcacc atggttaaat
     5941 acttccttcc cgaggtcggc tttgtcatcg gcttttccca gtacggccgc cgcggagaaa
     6001 aacagaatct gatcgaaagg ctgcaggcca tggatttcat ccccctcatg ggcgtggccg
     6061 aaggcagctg caacctcgga ctacccctct ccgcctcggg ccaaaacctc gcctaccgcc
     6121 gcgaggcctt ccacgccgtc gggggctaca gccgcgtcgc ccatcgcgtc tccggagacg
     6181 atgtcctcct cctgcagctc atccgcagcc acaccgatta tcgcgtggtc ttcgcctccc
     6241 acccgggagc cttcgccagc tcggcgcccc agcccaccct ggctgatctc atcaatcagc
     6301 gcaaacgctg ggcttcgaac ggctcctttc agctcttcct aaatatccct ttctttgctt
     6361 atttaatcat ggtctacctc tataccgccg cactcttcgt cggggtgccc ctctcgctgc
     6421 tcctaggcac ccatgccggc ctctttgccc tctgcctggc cagcaagatc ggaggtgagt
     6481 gggccatctc tctggtcagc gcacaccgat atggcagaac cgacttgctc cgctggtttc
     6541 ccctctggtt tatcgtccag atcccctata tcgtttgggt cggcatgctc ggtagctttg
     6601 gaaatttcaa atggaaggac cgcaaacatg gagcagccct atgatcctgc ccgtgcttga
     6661 aaaggccccc gccgccctca gccacgcctg gcataaaaga cccctcaact ccagcaccga
     6721 ggtgcgcctg acccggctct gcacccagcg ctgccgacaa tgccaggtct atgaacgcac
     6781 gaccgaacca gcgagcatgg atatccatat gttccgccat atcgcccggg aactctcccg
     6841 gtatggcgcc tatatcggct tcatctccgg cggcgaagcc accatggtcc acgacctgcc
     6901 gcaaattctc ctcgagtcca ggaaaacctt ccccgtcgcc accaccctgg taaccggcct
     6961 ctacaacaag agcgaactga tccgcgacat cgcacagatc tgcctcgaca atcagatcaa
     7021 cattcagacc tccctcgacg gcttcggcga gaccggcgac ctcctgcgcg gggccaaaga
     7081 tttcagcgcc actgtgctgc gccacatgga gatgatctcc gccatgcgca gcagctcccg
     7141 ctccctgctc tactgcaata tcgttctcag ccgactcaac ctgccacagg ttcccgagct
     7201 gatcgcagca gccagggcta taggctggaa agccaccatc gggctttatc accatctcac
     7261 cgaaaccacc cgcgaggacg acaacctttc cgtccggccg ggcgaggatc tcgacaaact
     7321 ggtcgccttc ctcgataaca atcccgacat cctcaacctg aatgcctgga tccgcggcat
     7381 cgcccccttc atcagccgcg gcgaaaccac tccgtgccct tttgtggcct cgcgcttcct
     7441 ctccagccgg acaacaatca tggaaaatgg cgatcttcac ctctgctggg gcggaccggt
     7501 cggcaaccta ttcacccact cgatgaagga gattttttcc ggtgacgcct accgccagcg
     7561 cctggaggag taccaaaact gccgcggctg ctggaccacc tgttacaccc aacgctatct
     7621 gctcgtccat ccacgctccc tgggcgaagc cctcaacaac agccgcaaga tcgtccgcat
     7681 gcagaggagg cgcgggtgaa aaaacgactc ttccaggcca cattcccggc cctccggcgg
     7741 ctgttggcag gctcagctct tctgctgggg ctcatccagc ctggatcccc caaccccctc
     7801 tgcgcccagg agaacagcac cgccgggccc ctcgtttttg acgaaaccgc ggaatactca
     7861 ctcgcgttta aaggattggg cgccgcacag gcctccattc gctttttccc ggggacgcca
     7921 cagcgtatcg aagcccggat cgatacccgg ggcctgacca gcctgatctt ttcggtgcac
     7981 aacacctacc agagccacat cgacccgtca tccgggctgc ctatagtcgt ggaaaaaaat
     8041 atcgcccaaa gcaatatccg ccagcagctc tccatccgtt atgaccggcc ggacaatacc
     8101 gcggagagca gcacccccgc ttcctggtcc attctgccgg gcgcgatgga cctcttcacc
     8161 atgctcttcc agctgcgcag gatgtcgctc cctccgggcg caaaggtcgc cttcccactc
     8221 gatatcgaaa gccaggcctg gctcgcatcc ggccaggtgg aaaagggcgg agagatggag
     8281 agcccctgcg gccggctcga gacccgcagg ataaccctcc ttttcagccg catggaccag
     8341 ggttcccccc gcgcctggaa aaccgatctg ctcaccaacc gcatcgcccg tccggacggc
     8401 atgctgaccc tgcttctcgg cccgccacct gaacagatcc ccctgctcat ccagttcggc
     8461 ccgccgggcc aacaggtgag catgcgcatg aaaaactaca ccagggggaa tcgctcttgc
     8521 ccgtgatcat cctcttcaca ggcggactct atgccctcct catcgccctc ctgtatcgcc
     8581 atctcaggtg cctcttcgct gaggagcgac gcccgctgca ggacgagcct atggtcagcg
     8641 tgatcatcgc catccgcaat gaagcccccc atctcatcga ctgcctggaa tccctggccg
     8701 ccctcgacta cccccgcgac aggctcgaaa tcatcctcat cgatgacggc tccagcgacg
     8761 gctccgcaga actgctgcac gattttcacg accgccaccc ctgggtccag gccattcgtc
     8821 tggagggtga cgcgaagcag ctcccgggca aggcgggcgc ggtctaccgg ggcataaccg
     8881 gcagccgggg ggagatcatc gccatgaccg acgctgactg ccgcgtcccc cccggatgga
     8941 tccgccatct cctcgccgcc tttgatgacg gggtcggtct ggtcggggga ttcaccttcc
     9001 tcgagtccac ggcccggaaa cgcacgtgct ttgaggccgt ccaggccctc gactggcttt
     9061 ttctgctcgg cattgcggcg gcagccatta agatgggcaa acccctgacc tggatgggta
     9121 acaacatggc cttccgccgc cgcgcctatg aggatgtcgg cggatacccc gcccttggcc
     9181 acaacctcat cgaggatttc gccctgctta acgcgatctc acgagagacc gaatggaagg
     9241 tgcgcatcac cccgtcaaac caggccgccg tcctcagcct tccggtcggc tcgctctctg
     9301 cgctctataa tcagcgccgc cgctgggccc tgggcattcg ccaggtgcgc cccttcggca
     9361 aactcctgct ggtgaccagc ttcctcgccc atctcggggc aaccgccgga ttgatctgct
     9421 gccggccagc cgccctggcc accctcgtac tgctgctggc cggcgacctg gccgtctgcc
     9481 ggaaaatgag cagtacgacc gccaacaccc ctcttttacg ccatcttcct gcatttgaac
     9541 tcttgtattt cagctatacc ctgttcctgc cgctgctgat ccttttcgat cgccgcatcc
     9601 gctggaagga tcaaaacttc ccggcgcacg caaagaaaaa ccgcggcaca gcctaattcc
     9661 cgaggcccca tgtcctggat ccctcccctc ctcctccatt attacatcac ggaacgctgc
     9721 aacagccgct gcagcttttg tgatatctgg cggcagccgg ccggccctga cgccctgctg
     9781 gaggatgtgg aaagaaacct gcatgacgca gcccggctgg gcctgcgctt tgtcgatttc
     9841 accggtgggg aacccctgct tcatgcggac ttgccgcgca tgctccgccg cgcccgttcg
     9901 gagggcctgc gcacgaccct gaccaccaat gccatgctct atcccgaccg cgccgccgag
     9961 ctggtgggaa acgtcgattt tctccacttc tcgctcgatg cttccaccgc cgccgaccac
    10021 gaccgcctgc gcgggcgccc gctctttgac cgggtcatgg cgagtataga cctggccagg
    10081 cagcttggag aaaagcccga tctcctcttc actgcccttc ctgacaatct ccatcacctt
    10141 cccgggctgg ctgaattctg ccggcgcctc ggcctggtct tgattgtcaa tccggtcttc
    10201 tcgcaccgtg agaaacggga attaaatcct gaagatctgg cgtttataga tcagttcgcc
    10261 cgccgcccgt ttgtctacgt caaccgcgcc tttcacctcc tgcgcaaaag gggaggcaac
    10321 agcatcgtac agccccgctg ccgtgtcgtc gacagcgttg tcgtgatctc tccgcagaac
    10381 cagctgattc tcccctgcta ccatcacgag caggcccgca tcagccttgt agagggactc
    10441 gcagcctgct ggcacagcgc ggcggtgaac gaactgcgcg cgcagcaggg ccgatggccc
    10501 gcctgcgcgg gatgcaccct gaattgctat ttcgatccct ctttcgcatg ccggatcgat
    10561 gccctgttca tgcacagtat ggcggccaaa atcaagtacg cccgggacaa atatgtgcgc
    10621 gcccctctga tgcgcaccct ctcccgaacc gacccccggc cggcagccgg gatcatggcc
    10681 agcttaagca agccggacga cagccctgcg gaggcccgat gatcgcatcc ggtttctcag
    10741 ccccccctat gcccgccctc ctgttgatcg gctttttcct ctatgtcgag acccatttcc
    10801 gcatcggccg gctgcccagc cgctacttcc gccgccagcc cgaaatcctc ttcgatattc
    10861 cccaccgcgt cgctcccggc gccgacctgc ctctgcttct catcatcaag gacgcggacc
    10921 gctatcccgt cgagctcaac gacatcagtg tagatatcaa gcaggagggg aagatcctct
    10981 ccaccacccg gctgcctctc ggagatgaaa gggtggatca acccctctgg tggcgccgct
    11041 ttcccctgat tgtccgcggc ttcgagggtg aggttaccgt cgaaccgatg ctgactggcc
    11101 gctgcagcgg ccgtgccctc gaggtgcgcg tcgacaacta tcccacactc agccatgcac
    11161 ccctgaactg ccgcctggac cgggatcagc tgccgggcgc ggagcgctgg ttgtatggcg
    11221 acctgcacta tcacagccac ctcacctccg atcaggtgga attcggcgcc ccacccgcgg
    11281 cagccgcagc catggccgcg gcgatgggcc tcgatttctt cgccgtcacc gatcacagct
    11341 acgatctcga tgaccgctgg gatgactatc tccacaacga tgaggaactc gccaagtgga
    11401 agtggctgca ggatttcgtc cgggagtgga acggcggggg caggcatccc ctcatcattc
    11461 ccggcgagga gctctcggcg gggaacagcc gcggaggcaa tgtccacttt ctcctttata
    11521 atgagccgtc gttcttcccg ggctgggggg actcggcaga aaaatggctg cagaccaaac
    11581 cgcagtggac catcaggaag gttctggacc agctctctcc acaggcgctg gcctttgccg
    11641 gccatgcaga gtcggctccg ccccgcctgc agcgcctctt cctcggccgc gaccactggc
    11701 gcgccgccga ctatgaacac ccccgcctcg atggcgtgca gatgtggaac cgcctccgcg
    11761 atcccgcctt cagccgcggc ctggccgcct ggcagaaact tctcctcgcc ggccgccgcc
    11821 cggtcctgat cgggggcaat gacgcccatg gcgactttgg ccgctggcgc cagatctcgc
    11881 tcccctttgt ctcgctgaag gagaaagagg aacacctctt cggccaggtg cgcaccggca
    11941 tcatccgccc cgccggtaca gccaccctgc cagacctcct gcactccctg cgcgccggcc
    12001 gctgcctggt caccagcggc ccctccctct ggctcgaact gattactgag accggtcccc
    12061 cctgcacccc gggcgatacc tgtaccggcg cgccccgtaa gctgcacatc gcagccgatt
    12121 ccacccccgc cttcggcccc ctcgccaggg tcaccctctg catcggcgat ctgcagcgca
    12181 aaaaagaaac ccgcaggcct cttcccatcg gacctgcggg ttccatgaac ctgacgctgg
    12241 atgagcccgc ccgggaactc cccggacagg gctatctgcg gctggaggtg agcagcgatc
    12301 gcgagggcac ccccttccac tgcttctcca atcctatcta tctggcctgg cgctgatccg
    12361 cgccgtcccc tccttatatc agcttgatct ttttgatcat ctttttcaga ctgcggccgt
    12421 aaagttccgg ttccacctcg acatggcgct ccgccccttc accatggctc gcgctcttga
    12481 cggactcatt ggtcttcaag gcctcatgaa tgacaaagcg ctcatagctg ctcatcggcg
    12541 gcaaggccac cttcttgccc gtctggatcg cctccgctgc gcttttttcc gccaggcggc
    12601 gaagattgcc gaccggatag acgcgcagat caaattcgtt ctcggcgagc ttgtaggtct
    12661 tggtggcata ctccagactg cggtcgaaat gacgatgcac ctggcgccgc tcgttggtat
    12721 ccagattcaa caggctgacc ggcgccagtg aacc
//
LOCUS       DAOPWU010000067        12563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5101_length_12563_cov_5.284378, whole genome shotgun sequence.
ACCESSION   DAOPWU010000067 DAOPWU010000000
VERSION     DAOPWU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5101_length_12563_cov_5.284378"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKM23_05335"
     CDS             complement(<1..595)
                     /locus_tag="PKM23_05335"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNY90931.1"
                     /translation="MSAEAKSGARLCPNCKRLISVNAKECIHCGWKNYGLSGLGTGFE
                     KIIRSLGGLVPVLIGACVFLYIVALLMDLSALASPRGLFGLFSPGGEALYRLGMTGTY
                     PLAQGQWWTLFTAIYLHGGLLHILFNMIWLRNIGYAVEELFGTSRAFIIFTIAGVAGF
                     LASDLLRVAFTVGASGSIFGLLGALIYYARHRGGAFGE"
     gene            776..1864
                     /gene="ychF"
                     /locus_tag="PKM23_05340"
     CDS             776..1864
                     /gene="ychF"
                     /locus_tag="PKM23_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNY90932.1"
                     /translation="MKIGIVGLPFSGKTTLFNALTGAAADPAASAGKREAQHAIVKVP
                     DERLDKLDAIYQPKKKTPATIEYIDLSGLSADEQKKGGFSDQFLGQLRTVDAVLVIAA
                     AFHNDNVPHPLNSINLQRDFNLVQEEFILSDLGIIENRLQRLGKEMRVRKTDQHVKEE
                     HILQRCKSAIDALKPLRTLAFAPDEEILIRGYQFLSLKPHILVPNIDEQEIGRSEAIA
                     ADLAEWAAIPKTAVLPISARIEMEIQQLSDEEAELFRQDAGILQPAMDRLLRTSYELL
                     GLLSFFTVGEDEVRAWTIRGNTLAPQAAGEIHSDIERGFIRAEVVHYDDFIARGTMAK
                     CRAEAVLRLEGKEYIVKDGDIINFRFAV"
     gene            complement(1861..4524)
                     /locus_tag="PKM23_05345"
     CDS             complement(1861..4524)
                     /locus_tag="PKM23_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY90933.1"
                     /translation="MTPTLQIIHLVDPAVLQHVQDSFALQKSLSILTFDTRGEKLTRP
                     SSNCPLPAEMNPILGPFLDFILTNPPHFADLGLRDGNTVFASFFNGIFHRAILPVMVQ
                     RQALGAVQLVTVSDLDSFDAGRWRYILDGFAWNDVSYLAFLDAQPRMPLAELDRSTAD
                     FRTRLTEMLESGYSRFKKQQTGTTPPDWIGQTSPELVTNRIGDIIAASPSMAGVLHYD
                     SVEEMIGLNAIEHLTLDAAMQGELHETLTLGEAETTTEALVQTKDGLLLRVTWRIFLE
                     KDDEGHEVGLRWQIQRSTEVLTSRPESMPSPAQPPVREGTAAGNGLPVRDEPADPARR
                     RDRDKKQPAAAGAVAAEQNEGPPAPVEKSASGGEKQESGSGGEEGEGITPDMLGFMDE
                     LRYPLFAVGGDNRILVWNRQLVDLLRISAQAVANLDFANLLVGDSQKLWHQWLFEFRV
                     NPDITEAKPAGLLYVLDNSGEVYAVSLELAKTNLPAGQVITAAVRSCEKAAPPPATLH
                     YENGDEKPRREAEVEDEEPADSTGRGSGGLAAFSSVIGRQWPLFHEQLTQFIRPHELL
                     EAKRENARRLLEGADALSRLLQQLHYIAGDLQLNRAPVPIVRILRYAANIQERLFIQP
                     ADIVWLLPDEGVLVQGDTVQLYHAMVYLLDFVRRVMEEGTTLRIRVGTAAGAEGLPGW
                     GEEVKMAVIEADYTDHQSPWPEPDNWARGARTEEDFALGAMQAIVLAHQGMVRIMPLN
                     EERRCFQLFLPLASGAAQPAAGGATVLVIDDEPGIVQMNVLMLEHAGYSVLSAISGSE
                     GLRLLREHGGEIGAILLDWQLPDITCQDMAAEIARISQAPLILASGFLPDSEIKRVIE
                     RYDVRFLQKPYTLNQLVKAVAAAMEGGAPRR"
     gene            complement(4624..5385)
                     /locus_tag="PKM23_05350"
     CDS             complement(4624..5385)
                     /locus_tag="PKM23_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY90934.1"
                     /translation="MIGIEDIHFSYRDEHGIPRPVLNGISLNIEPGEAVAIMGANGSG
                     KSTLVKCCNGLLLPGKGRVVVDGLVTADEQSLAAIRRRVGMIFQNPDNQIVSTTVERE
                     IAFGLENLGLPYEEMHARVDEMLRRFDLTPFRLKSPHYLSGGEKQRLALAAVLAMRPR
                     YVILDEPTSLLDPQHRRQIIDLLLKLHHEGEIAWILVTQFPEEALLAGRLIILEQGRL
                     FRAGKPADLFAEVDELLAIGLEPPLSFYLDHLAGG"
     gene            complement(5382..6311)
                     /locus_tag="PKM23_05355"
     CDS             complement(5382..6311)
                     /locus_tag="PKM23_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNY90935.1"
                     /translation="MSLLVVGSMAYDDVITPAAEARDALGGSATFFAAAASYFTPVNL
                     VAVVGEDFKHEEIAFLRERGVDLDGLTVEAGKTFRWKGRYLANMNDRETIYTHLNVFE
                     RFDPRLPELYRESEFVFLANISPALQHSVISQVHRPKFVAMDTMNFWITGAPDALRET
                     LKLVDALVVNDSEVRELSGEDNLIIASRLVQQMGPRTLIIKKGEHGALMVHGESYFFC
                     PAFPVARLKDPTGAGDTFAGGLMGYLALSGKVTEENLRRAVVFGTALASFVVEDFSCN
                     RLRSLDRKEILGRVDALRAMTRFEKDSFWPAEE"
     gene            complement(6325..7983)
                     /gene="argS"
                     /locus_tag="PKM23_05360"
     CDS             complement(6325..7983)
                     /gene="argS"
                     /locus_tag="PKM23_05360"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNY90936.1"
                     /translation="MKTPQEYVQWALEQALAASGWEIPPGLYLGLERPKQEELGDLSS
                     PLAMTLARELRMAPRKAAEILVSHLPQDPDYLDRVEIAGPGFINFFYAKPCLQQAVLE
                     IIRAGENFGRSAMGQGRRIQLEFVSANPTGPLNIVSARAAAIGDVLCRCHRAAGYDAH
                     SEFYVNDAGRQIRLLGASLSARYMSELGRSEPVPEEGYHGEYLRDLAREIIEREGERY
                     AGMPAEERAALFSTIALEHMLGRQRASLDQYGVHYDHWFRESSLRESNAQQSILDAFA
                     QAGLSYEQEGALWFRSTSFGDEKDRVLVTREGEPTYFLIDIAYHEDKYRRGFEKVIDF
                     WGPDHHGYIDRMRAAILALGHPKESFAVSIIQQINLLRGGESVKMSKRAGQIIEMDEL
                     IEEVGTDAARFYFVDRRISQPMDFDIDLAKKQSDENPVYYVQMAHARICNIERFAIEQ
                     GLEVPPEADASPLGNAHEVQVIKKLLEFPEVIQRVVQHTEPHRVSNYAQELAAIFHRF
                     YHENRVVTEDVPLSLARLMLARATRRVLANAFSLLGISAPENMR"
     gene            complement(8053..8421)
                     /gene="rsfS"
                     /locus_tag="PKM23_05365"
     CDS             complement(8053..8421)
                     /gene="rsfS"
                     /locus_tag="PKM23_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNY90937.1"
                     /translation="MESQELARRIARLIWEKKGADLVIMDLRKLTSVTDFFVLCTVDS
                     DPQARAVMDHLSEQLLTEAVKPWHVEGTTGSKWLLLDFVDVVVHIFRPDARSFYGLER
                     LWGDAVFEEVNDQSYAHPES"
     gene            complement(8425..9501)
                     /locus_tag="PKM23_05370"
     CDS             complement(8425..9501)
                     /locus_tag="PKM23_05370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNY90938.1"
                     /translation="MAYQEPKKILVIRLSSIGDILLTTPLLRVLHRRFPEAQIDFVIK
                     TRFAELMRNNPHLHELLLLDTETGEEGLQELRRKIRMAGYDLVVDIHRNFRSFYLRRG
                     LPGAVIATYSKQMVRRFLLVRTGVNLYREVIPVHQRYIRALRRWGIEDDGLGLEFFID
                     PQAERLISLRLAERSNARPLVGIAPGAGFATKRWPAENFLEIARGLIREHQADILLLG
                     NKADRELTSPMASALGAGVYDWAGTLTLQQSAAAVASCDLLISNDTGLMHLACALRVP
                     VLAIFGPTTRELGFFPVGPESEVIENEELSCRPCSHLGGERCPKDHFRCMREILPAQL
                     LARAGRMLERRRGSQTEKPQQTGG"
     gene            complement(9512..10450)
                     /locus_tag="PKM23_05375"
     CDS             complement(9512..10450)
                     /locus_tag="PKM23_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNY90939.1"
                     /translation="MPRSLVTGGAGFLGSHLCEYLLGKGHQVIAMDNLLTGSIANIEH
                     LQSEEFKFIKHDVTEYIYLAGPIDFVFHFASPASPLDYLQLPIQTLKVGALGTHKALG
                     LAMAKNARFLLASTSEVYGDPLVHPQPEEYWGNVNPVGPRGVYDEAKRFGEALTMAYH
                     RYKEVDTKIVRIFNTYGPRMRPNDGRAIPAFVPQALRNQPITIFGDGSQTRSFCYVSD
                     LIEGIYRLMMSDQHEPVNIGNPHEMTIREMAEAIIRLTGSSSPLVTKPLPVDDPKVRQ
                     PDITLARRLLGWEPRVDLEEGLTRTIAWFREVLDKP"
     gene            complement(10462..11379)
                     /locus_tag="PKM23_05380"
     CDS             complement(10462..11379)
                     /locus_tag="PKM23_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNY90940.1"
                     /translation="MDTYVTIKLYPPPAGRDAAAALIDSAAARMQKIDSLASSYHPES
                     EIGWLHAHAGRPVLLLTPEMAAMLTAALHIDSLSSGAFTPAIGALTGLWGFGRREVMR
                     VPAPEERRQALLHTGRQGLRLRPEGLSFTDTLTRIDLGGVAKGFAVDEAIALLRRHGI
                     TDAQVDAGGDLRTIAGPRTAGKRKVYVRHPLNADAFYGRFPLDEGAVVTSGDYERFFE
                     EGGIRYHHLLDPWNGMPAGGCRSVTVVGAEAVDCDALATAVFVLGPEKGMELIESMAG
                     IEGLILYEEAGRLQESISKGLAQRFERLP"
     gene            complement(11492..12466)
                     /locus_tag="PKM23_05385"
     CDS             complement(11492..12466)
                     /locus_tag="PKM23_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNY90941.1"
                     /translation="MKIESIFGPVQGSLNDFERAFTEVLHSDIPLANQVVNYIAGLKG
                     KRLRPALVFLASQLHGERNPDSMRAAIVVELLHTATLVHDDVVDSSDLRRGKPTVNSI
                     WDNRVSVLVGDLLFSRTLTALLNLNSPAALAIISEAANRITEGELMQVEHEHDYNVSE
                     PVYFDLVAKKTAALFSACCELGALAMGDNAETRGRMKQFGENLGIAFQIKDDLLDYTG
                     KAAVLGKPVANDIRENKVTLPLIYALAASPAELCQRIVAMIEKEEMDESEIDEVVSFV
                     DDAGGLDYAEGMARNYSDAALAILDLYAPSETRDSLTKLVQFAIAREN"
BASE COUNT         2440 a         3741 c         4039 g         2343 t
ORIGIN      
        1 cctcgccgaa ggcgccgccg cgatggcgcg cataataaat cagggctccg agcaggccga
       61 agatggaacc cgatgccccg accgtaaagg cgacgcgcag caggtccgag gcgagaaagc
      121 cggccacccc ggcgatggtg aagataataa aggcccggga ggtgccgaag agttcctcga
      181 cggcatagcc gatgttgcgc agccagatca tgttgaaaag gatgtgcagg agaccgccgt
      241 gcagatagat agcggtgaag agggtccacc actgcccctg cgcgagggga taggtcccgg
      301 tcatgccgag gcggtagagg gcctcgccgc cgggtgagaa caggccgaag aggccgcgcg
      361 gggaggccag ggctgagaga tccatgagca gggcgacgat atagagaaag acgcaggcgc
      421 cgatgaggac gggcaccagg ccgcccaggg aacggatgat cttttcaaag ccggtgccga
      481 ggccggagag gccgtagttt ttccagccgc agtggatgca ctctttggcg ttgacggaga
      541 tgaggcgttt gcagttgggg cagagccggg ctccgctttt ggcttccgca gacatgtatt
      601 tttcctcgtt gagatgaagg ctgtctctaa tatgcaaaaa aaaccgcagt aaataaagga
      661 taaaattgcc ggggcggcgc gagcagcgga atgcgccgga tattttcctt gcaaatggcc
      721 ggccgaaagt ttatatttta tgtaagttta gcacattatt aaacggaatg gaaccatgaa
      781 aataggcatt gtcggtctgc ccttctccgg caagaccact ctcttcaacg ccctgaccgg
      841 agcggccgcc gatcccgccg cctcggcggg caaacgcgag gcccagcatg cgatcgtcaa
      901 ggtcccggat gaacggctgg acaaactgga cgcgatctat cagccgaaaa agaagacccc
      961 cgcgaccatc gagtatatcg acctcagcgg cctctcggcc gacgagcaga aaaaaggcgg
     1021 cttcagcgac cagtttctcg ggcagctgcg cacggtcgat gccgtcctgg tgatcgccgc
     1081 cgcttttcac aacgacaacg tgcctcatcc cctcaacagc atcaacctcc agcgcgattt
     1141 caatctggtc caggaagagt tcatcctcag cgatctgggg atcatcgaga accgcctgca
     1201 gcggctgggg aaggagatgc gcgtgcgcaa gaccgatcag cacgtcaagg aggagcatat
     1261 tttgcagcgc tgcaaaagcg ccatcgacgc cctcaagccc ctgcgcaccc tcgccttcgc
     1321 ccccgacgag gagattctca tccgcggcta ccagtttctc tcgctcaaac cgcacatcct
     1381 cgtgcccaat atcgacgaac aggaaatcgg ccgcagcgag gccatcgccg cggatcttgc
     1441 ggagtgggct gcgatcccca aaaccgcggt gctgcccatc tccgcccgca tcgagatgga
     1501 gatccagcag ctctcggatg aggaggcgga gctcttccgg caggatgccg gcatcctgca
     1561 gccggctatg gaccgcctgc tgcgcacctc ctacgagctg ctaggcctcc tctctttctt
     1621 cactgtgggt gaagatgagg tgcgcgcctg gacgatccgc gggaacaccc tggcgcccca
     1681 ggcggccggc gagatccatt ccgacatcga gcgcggattc atccgcgcgg aggttgtaca
     1741 ctatgatgat ttcatcgccc gcgggaccat ggcgaaatgc cgcgccgagg cggtgttgag
     1801 actggaaggc aaggaatata tcgtcaagga cggggatatc atcaatttcc gctttgcggt
     1861 ctagcggcgg ggggctcctc cctccatcgc ggcggccact gctttgacca gctgattgag
     1921 ggtgtagggc ttttgcagga aacgcacgtc gtagcgttcg atcacccgct tgatctcgga
     1981 atccggaaga aaaccgctgg cgaggatgag cggggcttgg ctgatcctgg cgatctcggc
     2041 ggccatatcc tggcaggtga tatccggcag ctgccagtcc agcaggatgg cgccgatctc
     2101 cccgccatgc tcccgtaaga gccgcagccc ttccgagccg ctgatggcgg agagcacgct
     2161 gtagccggca tgttccagca tcaggacgtt catctgcacg atgcccggtt catcatcgat
     2221 gaccagaacg gtagcgcccc ccgcagcggg ttgggcggcg cctgaagcga ggggcagaaa
     2281 gagctggaag cagcgccgct cctcgttcag gggcatgatg cggaccatgc cctggtgtgc
     2341 gaggacgatc gcctgcatag cgccgagggc gaaatcctct tccgtccgag cgccccgcgc
     2401 ccagttatcg ggctccggcc agggcgattg atggtcggtg tagtcagcct cgatcacggc
     2461 cattttcacc tcctcacccc accccggcag cccctcggcg ccggcggcag tacccacccg
     2521 gatgcgcagc gtcgttccct cctccatcac ccggcgcaca aaatcgagaa gatagaccat
     2581 ggcatgatag agctggacgg tgtcgccctg caccagcacg ccttcatcgg ggagcagcca
     2641 gacgatgtcc gccggctgga tgaagagccg ttcctggatg ttggcggcat agcgcaggat
     2701 gcgtacgatc ggcaccggcg cccggttgag ctgcaggtca ccggcgatgt agtgaagctg
     2761 ctgcagcagg cgcgagaggg catccgcgcc ctcgagcagg cggcgggcat tttcccgttt
     2821 cgcctccagc agttcatgcg ggcggatgaa ctgggtgagc tgttcatgaa agaggggcca
     2881 ctgcctcccg atcacgctgc tgaaggccgc caggccgccc gagccgcgcc cggtggaatc
     2941 agccggctct tcatcctcga cttcggcttc ccggcgcggc ttttcatcac cgttctcgta
     3001 gtggagggtg gcgggtggag gcgccgcttt ctcgcaggag cggacggccg cggtgatcac
     3061 ctggccggcg gggaggttcg ttttggccag ctcgaggctg acggcataga cctcgccgct
     3121 gttgtcgagc acatagagaa gtccggccgg tttggcctcg gtgatatcgg gattgacgcg
     3181 gaactcgaag agccactggt gccacagctt ctgggaatcg ccgacgagga ggttggcgaa
     3241 atcgaggttg gccaccgcct gcgccgaaat cctgagcagg tcgaccaact gccggttcca
     3301 gaccaggatg cggttgtcac cgccgacggc gaaaagggga tagcgcagtt catccataaa
     3361 accgagcata tccggggtga taccctcgcc ctcctccccg ccgctcccgc tctcctgctt
     3421 ctcaccaccc gaggcacttt tttccaccgg ggccggtgga ccttcattct gctccgcggc
     3481 gacggctccg gcagcagccg gctgcttctt gtcgcggtcc cgtcgccgag cggggtccgc
     3541 aggttcgtcc cggaccggca gtccgtttcc ggcggctgtg ccctcccgga ccggcggctg
     3601 cgccggggag ggcatgcttt ccggccggct ggtgagtacc tccgtgctgc gctgaatctg
     3661 ccagcgcagt ccgacctcat gcccctcgtc atccttttcc agaaagatgc gccaggtcac
     3721 ccgcagcaag agcccgtcct tggtctggac cagggcctcc gtcgtggtct cggcctcgcc
     3781 cagggtgagg gtctcgtgca gttccccctg catggcggcg tcgagggtga gatgttcgat
     3841 ggcgttgagg ccgatcatct cctcgacgga gtcatagtgc agcaccccgg ccatggaagg
     3901 tgaggcggcg atgatgtcgc cgatgcggtt ggtcaccagt tcgggactgg tctgaccgat
     3961 ccagtcgggc ggcgttgtgc ccgtttgctg ttttttaaag cggctgtagc cgctctccag
     4021 catttcggtc agccgggtgc gaaagtcggc ggtggaacgg tcaagttcgg cgaggggcat
     4081 gcgcggttgg gcgtcgagaa aggcgagata gctgacatcg ttccaggcaa agccgtcgag
     4141 gatatagcgc cagcggccgg cgtcgaaaga gtccagatcg ctgaccgtca ccagctggac
     4201 cgcgccgagg gcctggcgct gaaccatcac cggcaggatg gcgcgatgaa agatgccatt
     4261 aaaaaaagag gcgaaaaccg tattgccgtc ccggagccca agatcggcaa agtgaggggg
     4321 attggtcagg atgaaatcaa gaaagggccc aagaatcggg ttcatttcag ccgggagcgg
     4381 gcaattcgag gagggcctgg tcagcttttc cccccgggta tcgaaggtca ggatggaaag
     4441 gctcttttgc agggcgaagg aatcctgcac atgctggagc accgcgggat ccaccagatg
     4501 gattatttgc agggtcggcg tcatcttgct ggttcaggcc atcgtttgca cagtgtgaat
     4561 gccttttgta cacccgaata taggtgcaaa aatattaaaa atcaacaggt gaggcaaaaa
     4621 gaatcagccg cccgccagat gatccagata aaaggagagc ggcggctcaa ggccgatggc
     4681 cagcagttca tcgacctcgg cgaagagatc ggccggtttg ccggcgcgaa agaggcggcc
     4741 ctgttcgagg atgatcagcc ggccggccag gagcgcctct tcgggaaact gggtgacgag
     4801 aatccaggcg atctctccct catgatggag cttgagcagc agatcgatga tctggcgccg
     4861 gtgctgggga tcaagaaggg aggtgggctc gtcgaggatg acatatctcg gccgcatggc
     4921 gaggacagcg gctagggcga gacgctgttt ctctcctccg gaaagatagt ggggcgattt
     4981 caggcggaag ggtgtgagat caaagcgccg cagcatttcg tccacccgcg cgtgcatctc
     5041 ctcgtagggg aggccaaggt tttccagacc gaaggcgatt tcgcgctcga ccgtagtcga
     5101 gacgatctgg ttgtcgggat tctgaaagat catccctacc cgtctgcgga tggcagccag
     5161 gctctgctcg tcggcggtga ccagtccgtc caccaccacc cggcctttgc cgggcaggag
     5221 caggccattg cagcatttta ccagggtgct cttgcccgag ccgttggccc ccatgatcgc
     5281 caccgcctcg ccgggctcga tgttcaggct gatgccgttc agcaccggac ggggaattcc
     5341 gtgctcatcc cggtaggaga aatggatgtc ctcaatcccg atcactcttc agccggccag
     5401 aaggaatctt tttcgaatct ggtcatggcg cgcagggcat caacgcggcc gaggatctcc
     5461 ttgcgatcga ggctgcgcag ccggttgcag ctgaaatcct cgacgacgaa ggaggccaga
     5521 gcggtgccga agaccacggc gcggcgcagg ttctcctcag tgactttgcc gctgagggcg
     5581 agatagccca tcaatccccc cgcgaaggta tccccggccc cggtcggatc cttgaggcgg
     5641 gccaccggga aggcggggca gaagaaatag ctttcgccgt gcaccatcag ggcgccgtgc
     5701 tctccctttt taatgatcag ggtgcgcggc cccatctgtt gcaccagcct gctggcgata
     5761 ataaggttat cctccccgga gagctcccgg acctcggaat cgttgacgac cagggcatcg
     5821 accagcttga gggtttcacg cagggcatcg ggggcgccgg tgatccagaa attcatcgta
     5881 tccatggcga cgaatttggg cctgtgcacc tgggaaatga cgctgtgctg cagtgccggg
     5941 ctgatgttag ccagaaagac gaattcgctc tcacggtaaa gctccggcag gcggggatcg
     6001 aaccgttcaa agacgttgag atgggtgtag atggtctcgc ggtcgttcat gttggccagg
     6061 tagcgcccct tccagcggaa ggtcttgcct gcctcgaccg tgagtccgtc cagatcgacc
     6121 ccgcgctcgc gcaggaaggc gatctcttcg tgtttgaaat cctcccccac caccgcgacc
     6181 agattgaccg gggtgaaata gctggcggcc gcggcaaaaa aggtggccga gccgccgagg
     6241 gcatcccggg cttccgccgc cggggtgatt acatcatcat aggccatgga accgacaacc
     6301 agcagactca tctcgtgtga actcctatct catgttttca ggggccgaga tcccgagcag
     6361 ggaaaaggcg ttagccagca cccgccgcgt cgcccgcgcc agcatcaagc gcgccagcga
     6421 gagcggtaca tcctcggtga ccacccggtt ctcgtgataa aaacggtgaa aaatcgccgc
     6481 cagctcctga gcatagttgc tgacgcggtg gggttcggta tgctgcacca cccgctggat
     6541 gacctcggga aattcgagca gctttttgat cacctgcacc tcatgggcat taccgagggg
     6601 actggcatcc gcctcggggg gaacctcgag cccctgctcg atggcaaagc gctcgatgtt
     6661 gcagatgcgc gcgtgggcca tctgcacgta atagaccgga ttctcatcgc tctgcttttt
     6721 ggccagatcg atgtcgaaat ccatcggctg cgatatgcgc cggtcgacaa aatagaatcg
     6781 cgcggcatcg gtgccgacct cctcgatcag ctcgtccatc tcgatgatct ggccggcgcg
     6841 tttggacatc ttgacgctct cgccgccgcg cagcaggttg atctgctgga tgatgctgac
     6901 tgcaaagctc tccttgggat ggccgagggc gaggatggca gcgcgcatac ggtcgatata
     6961 gccatgatgg tcgggccccc agaaatcgat caccttctca aaaccgcggc ggtacttgtc
     7021 ctcgtgatag gcaatgtcga tgagaaaata tgtcggttcc ccttcgcggg tcaccagcac
     7081 ccgatccttt tcatccccga agctggtcga gcggaaccag agcgccccct cctgctcgta
     7141 gctgagcccg gcctgggcga aggcatcgag gatgctctgc tgggcattgc tctcgcgcag
     7201 gctgctctcg cggaaccagt ggtcataatg gaccccgtac tggtcgaggc tggcgcgctg
     7261 gcggccgagc atgtgctcca gggcgatggt gctgaagagg gccgcgcgct cctcagccgg
     7321 cattccggca tagcgctctc cttcgcgttc gatgatctcg cgggcgaggt cgcgaagata
     7381 ttcgccatgg tacccctctt cggggaccgg ctcggagcgg cccagctcgc tcatatagcg
     7441 ggcgctcagg gaggcgccga ggaggcggat ctggcggccg gcgtcgttga cgtagaactc
     7501 actgtgtgcg tcgtagccgg cggcgcgatg gcagcggcag aggacgtcgc cgatggcggc
     7561 ggcgcgagcg ctgacgatat tgagggggcc ggtcgggttg gcgctgacga actcgagctg
     7621 gatgcggcgg ccctgcccca tggcgctgcg gccgaaattt tcaccggcgc ggatgatctc
     7681 cagcaccgcc tgctggaggc agggtttggc gtagaaaaaa ttgatgaatc ccggcccggc
     7741 gatctcgacg cggtcgagat aatccggatc ctggggcaga tggctgacca ggatctcggc
     7801 cgccttgcgc ggcgccatcc tcaactccct ggccagggtc atggccaggg gcgaggaaag
     7861 atcgcccagc tcctcctgtt tggggcgctc caggccgagg taaaggcccg gggggatctc
     7921 ccatccgctg gcggcaaggg cctgctccag cgcccactgc acatattcct ggggtgtttt
     7981 catgaggttg ttcctgtgaa gaaaaggtct gatccggtcc ctcccctgcg cagcaagtcc
     8041 cgggagttga ttttatgatt ccggatgggc ataactttgg tcattgacct cttcaaagac
     8101 ggcatctccc cagaggcgct cgaggccata gaaggagcgg gcgtcgggcc ggaagatgtg
     8161 gaccacgaca tcgacgaaat cgagcagcag ccatttggag ccggttgttc cctcgacatg
     8221 ccagggtttg actgcctcag tcagcagctg ttcggaaaga tggtccatga cggcgcgcgc
     8281 ctgcgggtcg ctgtcgaccg tgcaaaggac aaaaaaatcg gtgacgctgg tcagtttgcg
     8341 cagatccatg atgaccagat ccgccccttt tttctcccag atcaggcggg cgatgcgccg
     8401 cgccagttcc tgactttcca agccttatcc tccggtttgt tgaggttttt cagtttggct
     8461 cccccgcctg cgctcgagca tgcgccccgc gcgggcgagg agttgcgcgg gcaggatctc
     8521 ccgcatgcag cgaaagtgat ctttcgggca gcgctcgccg cccagatggc tgcacggccg
     8581 gcatgacagc tcctcatttt cgatgacctc gctctccggg cccaccggaa aaaaaccgag
     8641 ctcccgggtc gtcggcccga aaatggcaag gacggggacg cgcagggcac aggcgagatg
     8701 catgaggccg gtgtcgttgc tgataagcag gtcgcagctg gccacagccg cggcgctctg
     8761 ctgcagggtg agggtgccgg cccaatcgta gaccccggcg ccgagggccg aggccatcgg
     8821 actggtcagc tcccggtccg ccttgttgcc aagcagcagg atgtcggcct gatgctccct
     8881 gatcagcccg cgcgcgatct cgagaaaatt ttccgccggc cagcgtttgg tcgcaaaacc
     8941 cgcaccgggc gcgatgccaa ccaggggccg ggcgttcgat cgttccgcca ggcgcagcga
     9001 aatcaggcgt tccgcctggg gatcgatgaa aaactccagg cccaggccgt catcctcgat
     9061 gccccatcgc ctcagggccc ggatatagcg ctggtggacc gggatcacct cgcggtagag
     9121 attcactccc gtcctgacca ggaggaaacg ccgcaccatc tgcttgctgt aggtggcgat
     9181 aacggccccg ggcaggccgc ggcgcaggta aaagctgcgg aaattcctgt ggatatcgac
     9241 gaccagatca taacccgcca tgcggatctt cctgcgcaat tcctgcaatc cctcttcccc
     9301 ggtctcggta tcgaggagca gaagctcatg aagatgagga ttgttccgca tcagttcggc
     9361 gaagcgcgtc ttgatgacaa aatcgatctg agcctcgggg aaccgccggt gcagcaccct
     9421 cagcaggggc gtggtgagca ggatgtcacc gatggagctg aggcggatga cgagaatttt
     9481 tttaggttct tggtaggcca taccgctcca ctcaaggctt gtccagaact tctctgaacc
     9541 aggcgatggt ccgggtcaat ccctcctcga gatcgaccct gggttcccag cccagcagtc
     9601 tgcgcgccag ggtgatgtcg ggctggcgca ccttggggtc atcgacaggc aggggtttgg
     9661 tcaccagtgg gcttgagctg ccggtcaggc ggatgatcgc ctccgccatc tcgcggatgg
     9721 tcatctcgtg gggattgccg atattgaccg gctcgtgctg gtccgacatc atcaggcgat
     9781 agatgccctc gatgaggtcg ctgacgtagc agaagctgcg cgtctgggag ccgtctccaa
     9841 agatggtgat cggctgattg cgcagggcct ggggaacgaa ggccgggatg gcgcggccgt
     9901 cattgggccg catgcgcggg ccgtaggtgt tgaaaatgcg cacgatcttg gtgtcgacct
     9961 ctttgtagcg atgataggcc atggtcaggg cctcgccgaa gcgtttggcc tcgtcataga
    10021 ccccgcgcgg gccgacggga ttgacatttc cccagtactc ttccggctgg gggtggacca
    10081 gcggatcacc atagacctcc gaggtggagg cgaggagaaa gcgggcgttc ttggccatgg
    10141 ccaggccgag ggccttgtgg gtgccgaggg cccctacctt gagggtctga atggggagct
    10201 gcagatagtc gaggggactg gccggcgagg cgaaatgaaa gacaaaatca atggggccgg
    10261 ccagataaat gtactcggtg acgtcgtgct tgatgaattt gaactcttcg gactgcaggt
    10321 gctcgatgtt ggcgatgctg ccggtgagca gattgtccat ggcgatgacc tggtgcccct
    10381 tgccgagcag atactcgcac agatgggagc ccagaaagcc ggctccgccg gtgaccagtg
    10441 atcttggcat gttacctccc gttacggcag ccgctcgaag cgctgcgcca gacccttgct
    10501 gatgctctcc tgcaggcgcc ccgcctcttc gtagagaatc agcccctcaa tccccgccat
    10561 ggattcgatc aactccatcc ccttttcggg acccagcaca aagacggctg tagccagggc
    10621 gtcgcagtcg acggcttcgg ctccgacgac ggtgacgctg cggcagccgc ccgccggcat
    10681 tccgttccag ggatcgagga gatggtgata gcggatcccc ccctcttcaa aaaagcgctc
    10741 atagtcgccg gaggtgacca ccgccccctc gtcaagcgga aagcgtccat aaaaggcatc
    10801 ggcgttgagg ggatggcgta cgtagacctt gcgcttgccc gccgtgcgcg ggccggcgat
    10861 ggtgcgcaga tccccgccgg cgtcaacctg ggcatcagtg atgccatgac gccggagaag
    10921 ggcgatggcc tcatcgacgg caaaaccctt ggcaaccccg cccaggtcga tccgggtgag
    10981 ggtgtcggtg aaggagagcc cctccggacg cagacgcagc ccctgccggc cggtgtggag
    11041 cagggcctgc cggcgctctt caggagcggg gacccgcatg acctccctgc ggccgaaccc
    11101 ccacaacccg gtgagggcgc cgatggccgg ggtgaaagcc ccggaactga ggctgtcgat
    11161 gtgcagggcc gctgtcagca tcgccgccat ctcgggagtc agcagcagaa ccggccggcc
    11221 ggcatgggcg tgcagccagc cgatctcgct ctccggatgg tagctgctgg cgagggaatc
    11281 gatcttttgc atcctcgccg cagccgaatc gatgagcgcg gccgccgcat cgcggccggc
    11341 agggggcgga tagagcttga tcgtgacgta ggtatccatc agaaagaagg atttctctac
    11401 agccgtgatt gagttccgtc cgcatgaggc aagcaggcaa aagatcagaa tcgctgcact
    11461 tgggagggcg agggcaggaa gagaggcagc cctaattttc acgcgcaatc gcaaactgga
    11521 ccagctttgt cagggagtcg cgcgtctcgg agggggcata aagatcgagg atagccagcg
    11581 ccgcgtcgct gtagtttcgg gccattccct ccgcatagtc gaggccgccc gcatcatcca
    11641 caaaggagac cacctcgtcg atctcggatt cgtccatctc ctccttttcg atcatcgcca
    11701 cgatccgctg gcagagttca gccggcgagg cggcgagggc atagatcagg ggcagggtaa
    11761 ccttgttctc gcgaatgtcg ttggccacag gcttgcccag gactgcagct ttgcccgtgt
    11821 aatcaagcag gtcatccttg atctggaagg cgatgccaag attttcaccg aactgcttca
    11881 tacggccgcg cgtctcggca ttgtcaccca tcgcgagcgc gcccagttca cagcaggcgg
    11941 agaagagggc cgctgtcttt ttggccacca ggtcgaaata gaccggctcg ctgacgttgt
    12001 aatcatgctc atgttcgacc tgcatcagct cgccctcggt gatgcgattg gcggcctcag
    12061 agatgatggc gagggcggca gggctgttca gattgagcag ggcggtgagg gtgcgggaga
    12121 aaagcaggtc accgaccagg actgagacgc ggttgtccca gatcgagttg actgtcggct
    12181 tgccgcggcg caggtcggag ctgtccacca catcgtcgtg taccagggtt gccgtatgca
    12241 ggagttcgac cacaatggcg gcacgcatcg aatccggatt cctttcacca tggagctggc
    12301 tggcaagaaa aacgagcgcc gggcgcagac gtttgccctt gagcccggcg atatagttca
    12361 caacctgatt ggcgagcgga atatcggagt gaagtacttc tgtaaaggcg cgttcgaaat
    12421 cgttcagcga cccctgtacg ggtccgaaaa tgctttcgat tttcacaaga aatcctgttc
    12481 aaccctgagt gaaggtacga atggcagatt tgatagataa tgtaataata atagttaaat
    12541 tagtcaacag ctaactcagc agg
//
LOCUS       DAOPWU010000068        12423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5179_length_12423_cov_4.115864, whole genome shotgun sequence.
ACCESSION   DAOPWU010000068 DAOPWU010000000
VERSION     DAOPWU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5179_length_12423_cov_4.115864"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PKM23_05390"
     CDS             <1..597
                     /locus_tag="PKM23_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNY90942.1"
                     /translation="DQLLALSMITTQQYQQLLGAYSGVKATAVAKTDVTADLPLPVNV
                     GIGFAYSGIENLLITGDVALSQWSAWDVIEVNDDDGNKINELTMNWEDGIRAGLALEY
                     SLALANAKLRASFYSEPAAPVAETMNPTIPDINRRNVVVLGFGLPVGPFEAGLMYEHM
                     FIGDKTVEWSPETPPFHNLGGLYTMTVNNIMFGLDYNF"
     gene            768..1619
                     /locus_tag="PKM23_05395"
     CDS             768..1619
                     /locus_tag="PKM23_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90943.1"
                     /translation="MRIAPLALLLGLLLLACDDESGIKPPSQLSGFTGTITFTGTWPE
                     DTDQIRLAAALRFPPSTIADVLLGEALPVGGDSLHYTFYTDPVALQAVGVVWKEKGQG
                     WDATNIIGYYFPTENHLTPGAVTISRKDEMIQDIDITCDLTKVKRHVNSAIGGMLRVQ
                     GAWPPGASKVVVAASTAPLLPTSLLDLFFSFPIDAGFDSTRYIISVQPGTYKLVAALV
                     IEESQSIGLESIKGAYYSFSGIKIINPETKKLDVDLNLIFPAGSAKAETRDSQTGPRS
                     LQHRRGE"
     gene            1623..3767
                     /locus_tag="PKM23_05400"
     CDS             1623..3767
                     /locus_tag="PKM23_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY90944.1"
                     /translation="MRNRLLFWLTAALLLPGAILAHTGRISGRVIDVKSREPIPGANV
                     MIQGTVLGANSDAEGRFSINSLAPGSYELRVSAVGYLVFNRRGIALTADQTVTLDVTL
                     EPTLIESGEVVVSASKRRQSLEDSPTSVGVMTTRELAQKNQVYLDRVLEHASGVNFIG
                     SQINIRGSSGYNYGAGSRVLMLIDGVPVMPGDSGDIKWSMVPASQVDHVEIVKGAGSA
                     LYGSSALGGVVNVITKEATAKPVTHVRFSAGFYDKPLYAEWRWTDRLLHFDDLDVDHS
                     RRIGKSNVMVALGRHQSTGYAQNGWYQRLNGLFKWQRQLTPKANLTLSSNFETNDNGT
                     GLMWRSQSEALSVAPEAIGDKVKSDKFGFNLFHRWAASSRFALNTRLSYFRNFWKNYF
                     HDNLNASQANRIGLEIQGDYQFSNINALTFGTEESWDIVESGLVGDHTQYVISGYIQN
                     ERRLLAGLALTLGARYDLQRVDTGFQDENLSPKVGLVWHARPWLSLRGSSGSGFRAAS
                     MSERFSNSVYSGLQLIPNPDLKSETAWSHEAGATLILLPMLSLDISTFRSDYWDLIEP
                     EPTVTQEIQFTNVTRARISGIETGLRLQPGIPGLSLQSAYTWMDPRDLELDQTLAYRP
                     RQISQTTLLYTAGPFEISADYRYISRLDNVKLYPSEDRVAQKTLDLHLTWHYRDWSLS
                     GHINNARNHNHTQMERTLQPIRSYMLTLSGTI"
     gene            3894..5564
                     /locus_tag="PKM23_05405"
     CDS             3894..5564
                     /locus_tag="PKM23_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNY90945.1"
                     /translation="MRTPIGVLMILALALCGCRTQADFPARAAATAVQAELDKLAPVA
                     LDCDLTSLSPADRMALGEIIRAATVMDQLFLRQVHPDNPALLKKLEGDKSPENAPYLE
                     MFRLMFGPWNRLDHDAPWLTGEAKPAGAGFYPHDLTRDQFEAWIAAHPEDKAAFEGTF
                     TVIRREGTRLAAVPFHTYWQPLVEEAAKHLEAAANFSDDPTLVNFLRLRAQGLRTDDY
                     YESDMAWMDLAGDLEVVIGPYEVYEDNLFNYKAAYEAFVCLVDHAESKKLEAIAGHLN
                     AMEANLPIPDQYKNFGRGSSSPIKVVQELFSAGDTKAGVQTTAFNLPNDERVREAKGS
                     KKVMLKNIAKAKYEKCWIPIVVTVLAEKPLAAVSFDAYFNHVLMHEVSHGLGPGMITL
                     ESGEKIDGARKLKELYSVLEECKADILGIINMKLMMEKGIFPAEMEYGMYASYLGGML
                     RSIRFGINEAHGGGVAIQWNYCFDRGAFLLDGEGKLTLDETRVLPALYSLAQEVLMFQ
                     ATGDYEGAKEFVEKYRVMTPVMKGMVEALKKVPIDIRPSYPVLTELGL"
     gene            complement(5599..7116)
                     /gene="glpK"
                     /locus_tag="PKM23_05410"
     CDS             complement(5599..7116)
                     /gene="glpK"
                     /locus_tag="PKM23_05410"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNY90946.1"
                     /translation="MNSYIGALDQGTTSTRFIVFDRLGTIIASAQREHRQIYPRPGWV
                     EHDPDEIWQNSCLVIEEAMMRARLSAAQLEAIGITNQRETTVMWGRQSGVPLYNALVW
                     QDMRTDRIIAGLAAEGGKERFRAATGLPLATYFSAPKIKWLIENVPAVAAAVREERAL
                     FGTIDTWLLWKLSGGRCHATDVTNASRTMLLDLASLRWDEAIASAMGIPGHILPEVRP
                     SSGFFAEAAAPDVIAGVPLLSLIGDQQAALAGQACFQPGQAKNTYGTGCFMLLHTGAR
                     PMASAHGLLTTVAGQRAGRPAAYALEGSVAIAGALVQWLRDNLGLISSAPEIETLARS
                     VEDNGGLYFVPAFSGLYAPHWREEARGVIAGLTRFANRGHLARAVLEATAFQTREVLD
                     AMIKDSGIGLAELKVDGGMVANQLLMQFQADLLDVPVVRPKVTETTALGAAYMAGLAC
                     GYWRDEEELERNWQVDKVWRPEMGEEERARLYSLWRKAVERTLNWVEPDSPAAEP"
     gene            complement(7137..8534)
                     /locus_tag="PKM23_05415"
     CDS             complement(7137..8534)
                     /locus_tag="PKM23_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNY90947.1"
                     /translation="MPTRQIVPEEWFSEKSALDRRLGLTEAFSILIGRIIGSGIFRTP
                     APIMILVGSVSMFYGVWLLGGIATLLGAFVYAEMAAMMPKSGGPYAFLRAAYPPVWAF
                     LRGWAMFFVSETGAIAAVSIVFAEYSNSLYRLLTGAAFSRGVEVLIALAIIWLLTLVN
                     CFGVALGGKVQNILSLLKLIALGMVIGVCFASGGSSTHFSQPFWPDKFTWGSLLAMGA
                     AMRYAFFAFSGWEGATYVAEEVKNPRKNLPLSLILGLTGVMLIYLAANSAYLFQLPAA
                     SIAESKWVAVDAVEAAVGGIGGILVSLAVMLNTFGSVSSQVLVKARGVYAMARDGLFF
                     RWLGEVHPRYKTPNRALIVQGLWATVLLFAAGFAAHAYETIIDFFSFTSSIFNISTFA
                     AVYVLRRKYPDAPRYYKAWGYPVTLILVLIIQVWFMVTTLITAFIPSLVGIALTSTGL
                     IYYYFNTRTGAAVGK"
     gene            complement(8609..11929)
                     /locus_tag="PKM23_05420"
     CDS             complement(8609..11929)
                     /locus_tag="PKM23_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNY90948.1"
                     /translation="MSTRIQKVIRASAGTGKTYRLSLEYIALLLRCRGEGIRFEEILV
                     ITFTRKATAEIRDRIFSQMKSLSSDDADAAGLRANLEPLLGRHWQESDRQWLLQVREE
                     MLTNKSQVQISTIDAFVNRLFKSIISPYIGLSSYAINSRMDPRYYASLYAYALEPENI
                     GIVKSLFERSQKRTLTDYEKLGHGLLDARYLFHCISRDNVSHSPQPQTAASHLAAFQA
                     RIDALLDRFEEYLAREKPFIEPRTALLGSWYEMLFGEAEAGSTWLDRARTRIKDAAFL
                     RQNFKQIIKEPKFWNGGSLLRKKDDKQLAVELKASLEEAMRELADFLYCSDFLQEEAE
                     LEQFAAMLLRKYDELRFRDQLFGYDDIAWYTYRYLYDPELSLVEEDSVTNAFYEILTS
                     RVRFLLIDEFQDTSLLQFNILRPLIREVTSGEGVRPYGGIIVVGDEKQSIYGWRGGER
                     DLLRALQMRLDESEPVRLDTSYRSSPVIIDFVNDFFGGAPLQQRLAAIGLDWPYESCR
                     TARLEMPGSILVAFRTYSDGDEAGDLASQRDACAEMVSKEIVPRLLEGTLRPSETAIL
                     ARENASLHAIADILDEYKIDYVLESSHSLLEHRAIKPAIYLLRYLATLNFDELLRFFR
                     SDYILAGGAELKEILLAFRDADADAAPRPLWQKLAARLPGNEAVQRCARFLKSAAGLD
                     LLTLCKRIFEDFSVTRIFTQENDSKNIAFFLQIVAGFCHNQREFEPSPSGFVRYCEQH
                     EEDEGWQQLGLEEVEAIRLLTIHKSKGLEFGSVFLFWPMTSPRDSHGSLRSYVRYDEQ
                     FQRIEEHALTYNLDGVLAASSAGGLVEREANRACIEELNAFYVAVTRARSNLAIYIVG
                     KNREGLEGLLASAEASEKSSAAQLILTAMHELLAGQGRLQSKDAEAAEARWGEWRGSQ
                     PSAARQEGLTPGALTGFLDPERSAWYRPDRERFEKERFLDYKSVYINKRRVDRGNLVH
                     HYLSFIEFDSPAARQRAARRALADYGSLLRPADIHLLIERVEAFITAHADYFSAARWE
                     RVFTEQTLYAPDGREERIDRMLVSDERREILIVDFKTGESHDQAQLDEYKHTVSSLPY
                     VHSRGYAVRGIFLEVEIG"
     gene            complement(11940..>12423)
                     /locus_tag="PKM23_05425"
     CDS             complement(11940..>12423)
                     /locus_tag="PKM23_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNY90949.1"
                     /translation="WPRSFMPQNQEMRDLIANMKLIPISDLIAGKRMLLCEDSIVRGT
                     QLRDFVARLYAFGGAEVHMRVACPPLLYGCPYLNFSRSRSLMDLAARRAIIELEGHFP
                     EKLEPFLDPSSPQYAAMTGKIQKRLGLTTLKYQTMPDMIKAIGLPREKLCTFCWTGSE
                     "
BASE COUNT         2413 a         3814 c         3859 g         2337 t
ORIGIN      
        1 gaccagctcc tggccctgag catgatcacc acccagcagt atcagcagct tctcggcgcc
       61 tactccggtg tcaaggccac ggcggtcgcc aagaccgacg tcacggctga cctgcccctg
      121 ccggtcaatg tgggcatcgg cttcgcctac agcggcatcg agaacctgct catcactggt
      181 gatgtcgccc tgagccagtg gtccgcctgg gatgtcatcg aggtcaacga cgacgacggc
      241 aacaagatca acgaactgac catgaactgg gaagacggca tccgcgccgg cctggccctc
      301 gaatacagcc tcgccctggc caacgccaaa ctgcgcgctt ccttttacag cgagcccgcc
      361 gcccctgtcg ccgagaccat gaacccgacc attccggaca tcaaccgccg caacgtggtc
      421 gttctcggct tcggccttcc ggtcggtccc ttcgaggctg gcctgatgta tgagcacatg
      481 tttatcggcg acaagaccgt tgaatggtcg cctgagaccc cgcccttcca taatctgggc
      541 ggcctctaca ccatgaccgt caacaacatc atgttcggtc tggactacaa tttctgatcc
      601 ccaagcggca tggggtgtgt atctgctgcg cccgccctgc cgccgacgtc tttacaggga
      661 gatcctcgca agggggtctc ccttttgttt ttgaaataat ccgcgttgca ggcaggttat
      721 aaaattatcg cggttccacg cactttcaca ccattcagga ttcccttatg agaattgccc
      781 cccttgccct tctgctcggc ctgctcctcc tggcctgtga cgacgagagc ggtatcaaac
      841 ctccctccca gctcagcggc tttaccggga ccatcacctt cactggcaca tggcctgagg
      901 ataccgacca aatccgcctc gccgccgcac tcaggtttcc cccctcgacc atcgccgatg
      961 tccttctcgg cgaagccttg cctgtcggtg gcgattccct ccattatacc ttttacaccg
     1021 atccggtcgc actgcaggcg gtcggcgtgg tctggaagga aaagggacag ggctgggatg
     1081 cgaccaacat catcggctat tacttcccca cggagaacca tctcactccc ggcgcggtga
     1141 cgatcagccg caaggacgag atgattcagg acatcgacat tacctgcgac ctcaccaagg
     1201 tcaagcgtca cgtcaacagc gccatcggcg ggatgctgcg cgtgcagggg gcctggcccc
     1261 cgggggccag caaggtggtc gtggcggcat cgactgcccc cctcctgcct accagccttc
     1321 tggatctctt tttttccttt ccgatcgatg ccggttttga ctcgacccgc tacattatct
     1381 ctgttcagcc cggcacctac aagctggtcg ccgccctggt cattgaggaa agccagagca
     1441 tcggcctgga gagtatcaag ggggcctatt acagcttcag cggaatcaag atcattaatc
     1501 cggaaaccaa aaaactggat gtggatctca atctcatctt cccggcgggt tctgccaaag
     1561 ccgagaccag ggattcacaa accggacccc ggtcactcca gcacagacgg ggggagtgag
     1621 ccatgcgcaa ccgccttctc ttctggctga cagcggcact gctgcttccc ggcgccatcc
     1681 ttgcccacac cggccggatc agcggccgcg tcatcgacgt caagagccgt gagcccatcc
     1741 ccggggccaa cgtcatgatc cagggcacgg tcctgggcgc caacagcgat gccgaggggc
     1801 gtttcagcat caattccctc gcgccaggta gctatgagct gcgcgtttca gcggttggct
     1861 acctggtctt caaccgccgg ggcatcgctc tgactgccga tcagaccgtc accctcgatg
     1921 tcaccctcga gccgaccctg atcgagagcg gtgaggtggt ggtctcggcc agcaagcgcc
     1981 gccagagcct tgaggattca ccgaccagcg tcggggtgat gaccacgcgc gagctggcgc
     2041 agaaaaacca ggtctatctc gaccgggtcc tcgagcacgc ctccggggtc aattttattg
     2101 gcagccagat caatatccgc ggctcctccg gctataatta cggcgccggc agccgtgtgc
     2161 tcatgctcat cgacggcgtg ccggtcatgc ctggcgacag cggtgacatc aagtggagca
     2221 tggtccccgc cagtcaggtc gatcacgtcg agatcgtcaa gggcgcggga tccgccctct
     2281 acggctcgag cgctctcggc ggagtagtca atgtcatcac caaagaggcc acggccaaac
     2341 cggtgaccca tgtgcgcttc tcggccggtt tttacgacaa accattatac gccgaatggc
     2401 gctggactga ccggctcctt cactttgacg atctcgatgt cgaccattcg cgccggatcg
     2461 gcaaatcgaa tgtgatggtc gccctcgggc gccatcagtc caccggctat gcccagaacg
     2521 gctggtatca gcgcctcaac ggcctcttca aatggcagcg ccagctcacc ccgaaggcca
     2581 acctgaccct gtcgagcaat ttcgagacca acgacaacgg caccggcctg atgtggcgca
     2641 gccagagtga ggccctctcg gtcgcccccg aagcgatcgg cgacaaggta aaatcggaca
     2701 agttcggatt caatcttttc caccgctggg cagcgagctc caggttcgcc ctcaacacgc
     2761 ggctttccta tttccgcaat ttctggaaaa attactttca cgacaatctc aacgcctcac
     2821 aggccaatcg catcggtctg gagatccagg gggattatca gttctccaac atcaatgccc
     2881 ttaccttcgg caccgaggag tcgtgggaca ttgtcgaatc cggactggtt ggcgaccaca
     2941 cccagtacgt catctccgga tacatccaga atgaacgccg gcttctggct ggtcttgccc
     3001 tcaccctggg ggcgcgctac gacctgcaga gggtcgacac cggctttcag gatgaaaacc
     3061 tgagtccgaa ggtggggctg gtctggcatg caaggccctg gctcagtctg cgcggctcgt
     3121 cgggcagcgg cttccgcgcc gcctcaatgt ccgaacgctt ctccaactcg gtctactcag
     3181 gcttgcagct gattcccaat cccgatctga aatcggagac cgcctggtcg cacgaggccg
     3241 gcgccacgct gattctcctt cccatgcttt cgctggatat cagcaccttc cgcagcgatt
     3301 actgggatct gatcgagcct gaacccacag tcacccagga aatccagttc accaatgtca
     3361 cccgtgcccg catcagcggg atcgagaccg gcctgcgcct gcaacccggg atccccggcc
     3421 tctccctgca gagcgcctat acctggatgg atccccggga tctggaactc gaccagaccc
     3481 tcgcctaccg gccgcgccag atctcgcaga ccaccctgct ctataccgcc ggcccatttg
     3541 agatcagcgc cgattatcgc tatatctcgc ggctggacaa cgtcaaactc tatcccagcg
     3601 aggaccgggt ggcgcagaag accctcgacc tccatctcac ctggcattat cgcgactggt
     3661 cgctcagcgg ccacatcaac aacgcgcgca accacaacca cacccagatg gagcgcaccc
     3721 tgcagcccat ccgcagctac atgctcaccc tgagcgggac gatctgatcc gccggcgccg
     3781 gacggctgca cgtccggcgc tcccctcatt taaggcttgc atttttctcc ctccgggctt
     3841 aaattcccga gttccacctt atcccgttga tgtactttaa tgcaggagtc cttatgcgca
     3901 ccccaatcgg agtccttatg atccttgccc tcgccctctg cggctgccgc acgcaggccg
     3961 actttccggc cagggcggcc gcaacggccg tccaggccga actggataaa ctcgcaccgg
     4021 ttgctctcga ctgtgatctc acatctctct cccctgcgga caggatggcc ctgggcgaga
     4081 tcatccgcgc ggctacagtg atggaccagc tcttcctgcg ccaggttcat cccgacaatc
     4141 ccgcccttct caaaaagctc gaaggtgaca aatcccccga aaatgccccc tatctggaga
     4201 tgttccgcct catgttcggc ccctggaacc gcctcgacca cgacgcgccc tggctgaccg
     4261 gagaggccaa gccggccggg gccggtttct atccccacga tctgacccgg gaccaattcg
     4321 aggcctggat cgccgcccac cccgaggaca aggcggcctt tgaggggacc ttcacggtga
     4381 tccggcgcga gggaacgcgc ctggcggcgg tgcccttcca cacctactgg cagcccctgg
     4441 tggaggaggc ggccaaacac ctcgaggccg cagcgaattt cagcgatgat cccaccctgg
     4501 tgaatttcct gcggctgcgc gcgcaggggc tgcgaacgga cgattactac gagagcgaca
     4561 tggcctggat ggacctggcc ggcgacctcg aggtggtgat cggaccctac gaagtctatg
     4621 aggacaatct cttcaattac aaggcggcct acgaagcctt cgtctgcctc gtcgaccacg
     4681 ccgaaagcaa aaagctcgag gctattgcgg gccatctgaa cgccatggag gctaatctgc
     4741 ccatcccgga ccagtacaag aatttcggcc gcggcagctc ctccccgatc aaggtggtgc
     4801 aggagctgtt ttcagccgga gacaccaagg cgggggttca gaccactgcc ttcaatctgc
     4861 ccaacgatga acgtgtgcgc gaggccaagg gatccaagaa ggtgatgctc aagaacatcg
     4921 ccaaggctaa atacgagaaa tgctggattc cgatcgtcgt gaccgtcctg gccgaaaagc
     4981 ctcttgccgc cgtctccttc gatgcctact tcaaccatgt gctcatgcat gaggtcagcc
     5041 acggcctcgg gccggggatg atcaccctgg agagcggaga aaagatcgac ggcgcccgca
     5101 aattaaagga actctattcg gtgctggagg agtgcaaggc ggacatattg ggcatcatca
     5161 acatgaagct gatgatggaa aaggggattt tccccgcgga gatggagtac ggcatgtacg
     5221 ccagctatct gggcggaatg ctgcgctcga tccgcttcgg gatcaacgag gcccacggcg
     5281 ggggggtggc catccagtgg aattactgtt ttgaccgggg agcttttctg ctcgacggcg
     5341 agggcaagct caccctggat gaaaccaggg tgctgccggc cctctacagt ctggcacagg
     5401 aggtattgat gttccaggcc accggcgact atgagggggc gaaagaattc gtggaaaagt
     5461 accgggtgat gaccccggtg atgaagggga tggtcgaggc ccttaaaaag gtgccgatcg
     5521 acatccgtcc ctcctatccc gtgctgaccg agctgggact ctagcctcaa atcagagccc
     5581 tggaggccgc tccggtcttc agggctccgc tgctggcgaa tcgggttcaa cccagttcag
     5641 ggtgcgttcc accgccttcc gccacaggct gtaaagtcgg gcacgttcct cctcccccat
     5701 ttccggccgc cacaccttgt cgacctgcca gttccgttcc agctcttctt catcccgcca
     5761 gtatccgcaa gccagccccg ccatataggc cgcacccaga gcggtggtct ccgtgacctt
     5821 tgggcgcacc acgggcacgt cgagcaggtc ggcctggaac tgcatgagga gttgattggc
     5881 gaccatgccg ccgtcgacct tgagttcagc gagcccgatc cccgagtcct tgatcatggc
     5941 atcgaggacc tcgcgggtct ggaaggcggt ggcttcgagc acggcccgcg cgaggtggcc
     6001 gcgattggcg aagcgggtca atccggcgat cacaccacga gcctcttccc gccagtgggg
     6061 ggcatagaga ccggagaagg cggggacgaa gtagaggccg ccgttgtcct ccaccgaacg
     6121 cgccagggtt tcgatctccg gtgcggagct gatcaggccg aggttatcgc gcagccactg
     6181 caccagggcc ccggcgatgg ccactgaacc ctcgagggca taggcagcag gccggccggc
     6241 gcgctggccg gcgaccgtag tgagcaggcc atgggcgctg gccatgggcc tggcgccggt
     6301 gtggaggagc atgaagcagc cggtgccgta ggtattcttg gcctggccgg gttggaagca
     6361 ggcctgtccg gccagggcag cctgctgatc gccgatgagg ctgaggaggg gaacgccggc
     6421 aatcacgtcg ggcgccgccg cctcggcgaa aaagccggag gatggacgca cctcgggcag
     6481 gatgtggccc gggatgccca tggcggaggc gatcgcttca tcccagcgca gagaggcgag
     6541 atcgagcagc atggtgcggc tggcgttggt gacgtcggtg gcatggcatc ggccgccgct
     6601 caacttccag agcagccagg tgtcgatggt gccaaaaagg gcccgctctt cgcgcacagc
     6661 ggcggcgacg gcgggaacat tttcgatcag ccatttgatc ttgggagcgg aaaagtaggt
     6721 ggccaggggc agcccggtgg cggcacgaaa acgctccttt cccccctctg cggccagacc
     6781 agcgatgatg cggtcggtgc gcatgtcctg ccagaccagg gcattgtaaa gggggacgcc
     6841 gctttggcgc ccccacatca ccgtggtctc gcgctggttg gtgataccga tggcctccag
     6901 ctgggcggcg ctgagccggg cccgcatcat ggcctcctca atgaccagac agctgttctg
     6961 ccagatttcg tccgggtcat gctccaccca gcctgggcgg ggatagatct ggcgatgctc
     7021 gcgctgggcc gaagcgatga ttgttcccag acgatcgaag acgatgaaac gggtggaggt
     7081 ggtgccctgg tcgagggcgc cgatgtatga gttcatggct ctcccttcgc cgcggctcat
     7141 ttcccaaccg ccgcgccggt gcgtgtattg aaatagtaat agatcaatcc cgtgctggtc
     7201 agggcgatgc cgaccagcga gggaatgaaa gcggtgatga gggtggtgac catgaaccag
     7261 acctggatga tcagcaccag gatcagggtg accggatagc cccatgcctt gtagtagcgc
     7321 ggagcatcgg gatatttcct gcgcaggaca tagactgcag cgaaggtgga gatgttaaag
     7381 atcgacgagg tgaaggagaa aaagtcaatg atggtctcat aggcatgggc ggcgaagccg
     7441 gctgcgaaga gcaggacagt tgcccagagg ccctggacga tgagggcgcg gttgggggtc
     7501 ttgtagcggg gatggacctc gccgagccag cggaaaaaga ggccgtccct cgccatcgca
     7561 tagaccccgc gcgctttgac cagcacctgt gaactgacgc tgccgaaggt gttgagcatc
     7621 accgccaggc tgacgaggat gccgccgatc ccgcccacag ccgcctcgac ggcatcgacc
     7681 gccacccatt tggattctgc gatactggcg gccgggagct gaaagagata ggcgctgttg
     7741 gcggccaggt agatgagcat cacgccggtg aggccgagga tgagcgagag gggcagattc
     7801 ttgcgcggat ttttcacctc ctcagccacg taggtcgccc cctcccagcc gctgaaggca
     7861 aaaaaggcgt aacgcatggc cgcccccatg gccagaagag aaccccaggt aaatttgtcc
     7921 ggccagaagg gttggctgaa atgggtgctg ctgccgccgc tggcgaagca gaccccgata
     7981 accataccga gagcgatcag cttgagcagg ctgagaatgt tctgcacctt gcccccgagg
     8041 gcgacgccga agcagttgac cagggttaaa agccagatga tcgccagggc gatgaggacc
     8101 tcgacgcccc ggctgaaggc ggccccggtg aggaggcggt agagggagtt gctgtattcg
     8161 gcgaagacga tcgagaccgc tgcgatcgcc ccggtctcgg agacgaagaa catggcccag
     8221 ccgcgcagga aggcccagac cgggggatag gcggcgcgaa ggaaggcata ggggccgccc
     8281 gatttgggca tcatcgccgc catctcggcg tagacaaagg ccccgagcag ggtggcgatg
     8341 ccgccgagga gccagacccc gtagaacatc gagacgctgc cgaccaggat catgatcggt
     8401 gcgggggttc tgaagatgcc agagccgatg atgcggccga tgaggatcga aaaggcctcg
     8461 gtcaggccga ggcggcggtc gagggcgctt ttttcgctga accattcttc gggaacaatt
     8521 tgcctcgttg gcacgctctc ctcctgactg gtcatttcct gctggtgatt tgaggtttct
     8581 gccgggctcg tcccggcggc ggcccgaatc aaccgatctc cacctcgaga aagatcccgc
     8641 gcacagcgta gcccctgctg tgcacatagg gaaggctgga gacggtgtgt ttgtactcat
     8701 ccagctgggc ctggtcgtgg ctttcaccgg ttttgaaatc gacgatgagg atctcccggc
     8761 gttcgtcgct caccagcatg cggtcgatgc gctcttcccg gccgtccggg gcatagaggg
     8821 tctgttcggt gaagacgcgt tcccacctgg cggcggagaa atagtcggca tgggcagtga
     8881 tgaaggcctc caccctttcg atgaggagat ggatatcagc ggggcgcagc aggctgccgt
     8941 aatcggcaag ggcccgccgg gcggcgcgct gccgggcggc gggtgaatca aattcgatga
     9001 acgatagata gtggtggacg agattcccgc ggtcgacgcg gcgtttattg atgtagacgc
     9061 tcttgtagtc gagaaagcgt tccttttcga agcgttcacg gtcgggccgg taccaggccg
     9121 atctctccgg atcgagaaag cctgtgaggg ccccgggggt cagcccctcc tgccgggcag
     9181 cggagggttg gctcccccgc cattcccccc agcgtgcctc cgccgcctcc gcatctttag
     9241 attgcaggcg gccttgtccc gcgagcagtt cgtgcatagc cgtgaggatc agttgcgcag
     9301 ccgaggattt ctcgctcgct tcggcactgg cgagcagccc ctccaatccc tccctgttct
     9361 tgcccacgat atagatggcc agattgctcc gggcccgggt cacggcgacg taaaaggcat
     9421 tcagctcctc gatgcaggca cggtttgctt cccgttccac cagtcctcct gcactcgaag
     9481 cggcgagcac cccatcgagg ttgtaggtga gggcgtgctc ctcgatgcgc tggaactgct
     9541 cgtcatagcg gacatagctg cggagactgc catgtgagtc ccgggggctg gtcatcggcc
     9601 agaagagaaa aacactgccg aactcaagcc ccttggactt gtggatggtg agcagccgga
     9661 tggcctcgac ctcctcgagt ccgagctgct gccagccctc gtcctcctcg tgctgctcgc
     9721 agtagcggac aaagccggag ggggagggtt cgaattcacg ctgattatgg caaaacccgg
     9781 cgacgatctg gagaaaaaag gcaatatttt tgctgtcgtt ttcctgggtg aagatgcggg
     9841 tgacggaaaa atcctcaaag atgcgtttgc acagggtcag gagatccagg ccggcggcgg
     9901 acttgagaaa acgtgcgcag cgctgcaccg cctcgttccc ggggaggcgg gcagccagct
     9961 tttgccaaag cggacgcggg gcggcatcag cgtcggcatc ccgaaaggcg aggaggattt
    10021 ctttcagctc tgctccgccg gcgaggatgt aatcggagcg gaagaacctg agcagctcat
    10081 cgaaattgag ggtggccaga taacgcagca ggtagatcgc cggcttgatg gcgcggtgtt
    10141 cgaggaggga atgagagctt tcgagcacat aatcgatctt gtattcatcg aggatatcgg
    10201 cgatggcgtg caggctggca ttctcgcgcg cgaggatggc ggtctcgctg gggcggagcg
    10261 tcccctccag cagccggggc acgatctctt tgctgaccat ctcggcgcag gcatcgcgct
    10321 ggctggcgag atcgccggcc tcgtcgccgt cgctgtaggt gcggaaggcg accagaatgc
    10381 tgccgggcat ctccagccgg gcggtccggc aggattcata aggccaatcg aggccgatgg
    10441 cggccagccg ctgctgcagc ggggcgcctc cgaagaaatc gttgacgaaa tcgatgatga
    10501 ccgggctgct gcggtaggag gtgtcgaggc ggacgggctc gctctcatcg aggcgcatct
    10561 gcaacgcccg cagcagatcg cgctcccccc cgcgccagcc gtaaatggac tgtttttcat
    10621 cgccgacgac gatgatcccg ccgtagggcc ggaccccctc cccgctggtg acctcgcgaa
    10681 tgagcgggcg caggatattg aactggagaa ggctggtatc ctggaactca tcgatgagga
    10741 gaaagcgcac ccggctggtg agaatttcgt aaaaggcgtt ggtgacggaa tcctcctcca
    10801 ccagggagag ctccggatcg tagaggtacc tgtaggtata ccaggcgata tcgtcgtagc
    10861 cgaaaagctg atcccggaag cgcagttcat catatttgcg caggagcatg gcggcgaact
    10921 gctccagctc ggcctcctcc tgcaggaaat cgctgcaata gaggaaatcg gccagctcgc
    10981 gcatggcctc ctcgaggctc gccttgagct cgacggcgag ttgcttgtca tcctttttgc
    11041 gcagcagtga gcccccgttc cagaacttgg gctccttgat gatctgttta aagttctggc
    11101 gcagaaaggc ggcgtccttg attcgggtgc gcgcccgatc cagccaggtg ctgcccgctt
    11161 cggcttcccc gaagagcatt tcgtaccagc tgccgagcag ggccgtacgg ggctcgatga
    11221 acggtttctc cctggcaaga tactcctcga agcggtcaag cagggcatcg atgcgggcct
    11281 gaaaggcggc cagatggctg gcggcggtct gcggttgggg gctgtggctg acgttgtcac
    11341 ggctgatgca gtggaagaga taacgcgcat cgagcaggcc gtgccccagc ttttcatagt
    11401 cggtgagggt ccgcttttgc gagcgttcaa agaggctctt gacgatgccg atattctcgg
    11461 gctcaagggc gtaggcgtag agggaagcgt aatagcgcgg atccatacgg ctgttgatgg
    11521 cgtagctgct cagtccgatg tagggcgaga tgatcgattt gaagagacgg ttgacaaagg
    11581 catcgatggt gctgatctgg acctggcttt tgttggtgag catttcctcg cgcacctgca
    11641 gcagccactg ccggtcgctt tcctgccagt ggcgtccgag caggggctcg agattggcgc
    11701 gcagtccggc ggcatccgcg tcgtcgctgc tgaggctctt catctgggaa aagatgcggt
    11761 cgcggatttc ggccgtggcc ttgcgcgtga aggtgatcac caggatctcc tcgaaacgga
    11821 tcccctcccc ccggcagcgc agcagcaggg caatgtactc cagcgagagg cggtaggttt
    11881 tgccggtgcc ggcgctggcg cggatgactt tttgaatccg tgtgctcatc gttccctttt
    11941 cactccgatc cggtccagca gaaggtgcag agtttttcac ggggcaggcc gatggccttg
    12001 atcatatcgg gcatggtctg gtatttcagg gtggtcaggc cgagccgctt ctggatcttg
    12061 ccggtcatgg cggcgtattg cggggaagag ggatcgagga acggctccag cttttccggg
    12121 aaatggccct ccagttcgat gatggcgcgg cgggcggcga ggtccatcag cgagcgcgag
    12181 cgggaaaagt tgagataagg gcatccgtag agcaggggcg ggcaggcaac gcgcatatgc
    12241 acctctgcac caccgaaggc gtagagccgg gcgacgaaat cgcgaagctg ggtaccgcgg
    12301 acgatggagt cctcgcagag cagcatgcgc ttgcccgcga tcagatcgct gatcggaatc
    12361 agcttcatat tggcgatgag gtcgcgcatc tcctgattct ggggcataaa gctgcgcggc
    12421 cag
//
LOCUS       DAOPWU010000069        12307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5248_length_12307_cov_5.063173, whole genome shotgun sequence.
ACCESSION   DAOPWU010000069 DAOPWU010000000
VERSION     DAOPWU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5248_length_12307_cov_5.063173"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PKM23_05430"
     CDS             <1..1286
                     /locus_tag="PKM23_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNY90950.1"
                     /translation="PASIGLGMPQKELKMVDLLLVIAKAKKFILRNVFIAGFLAVIIS
                     LLIPRWFSAKATLLPPTGDSSAMGISALLGNLPVSGFGMGGLTALSQETNLFLAILNS
                     RSLLGEIVEKFDLQKRYKTKTMEETIAALQDNITIQVNEDGTLSIRAQAKTPFLADSA
                     EVMQTKRLARDMTNALVDELDAINKRIKTDQARNTRLFIEKRYLQNLEDLKASEEALK
                     NFQQQNQAISLPDQTRAMITAAAELKAQITAKEIEADLMAKYVGKSHSDYIRLENELR
                     VLRRKYDEFKYGSDAQKLPKGALDEQEKDLFIPVDKVPDLGLRYVRLFREVTLQEKIL
                     EFLLPQYEQARIQEAKDTPTVQILDRAALPERKARPKRALIVLLAVFFVMVLSATHAL
                     LRERLETLRATETRRYEELQSFWKSLRSDWRFWKR"
     gene            1299..2717
                     /locus_tag="PKM23_05435"
     CDS             1299..2717
                     /locus_tag="PKM23_05435"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNY90951.1"
                     /translation="MRSGSEISALEARPSPAASAIIALTGLALALTVTRWPLLQALVA
                     VFTLLVVTLLFLRPEWSLVLFFSSGLIKEWLLLNVPLFARFDFTVAIFLLSATSLFFF
                     LLRRGYLFEFKIHPSMLPLFLFTALLIFSITYTPSFKYGSAKAFSFLLFNWALFLFPI
                     LLVREEAAAKRIMVILGTLAISATVYTLFSLVKSLFSGSIIFSYRASFLGINPISFAN
                     WIGAISLLLITYLPSIGKRWHRRAVILALGVLLLALLAANSRGPMFSFIITLILLLAL
                     RARKIPGRKLMQWVALGLLFVVIALLVLPEQVTSRYTDVITQEEGTRNYTFFTVNTRL
                     FAWNAALDMATARISSILMVVGSGGFSQVVYGQDVRLYPHNIFIEVFCELGIAGVVVL
                     LWYLASILGDVLQTLRRKMTERMRSLFMAFCMAALFNFVNAQFSGDLNDNRRLWFFLG
                     MVIALMQVIRSSSVEADHGSIR"
     gene            2701..4164
                     /locus_tag="PKM23_05440"
     CDS             2701..4164
                     /locus_tag="PKM23_05440"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNY90952.1"
                     /translation="MEVSDSRVVSNSLFIYAGRVINLVFNFFIFVYLANYLGESAFGR
                     FSIAITYVGTFDIIANFGLNQILVRELAGGRTEKSRLLGNGILLKGVITLLALACALA
                     ILPFMAYPRETMVVVWIILVNLIVSSKLSSTRTVFESIFQAQLRMAFPILCNILDNLL
                     FVLLIFLAAHFFNAGLTGMALIYGLCNLPGTFWLVGRFLRTNQVSWQPQWPVLRELLR
                     ESLPLAFYLFFSILTTKIDVLMLSWMRNEAEVGQYAAATRLVYPLSFLSTSLTISLFP
                     LLSKSFHERREEFDRLCRRGSKYVFLIGLFTSGVLAFAADPIIRTLYVPGYAACIPAF
                     RLLLISLGFSFLNFYFIDILISARRQKLVTGVMALAFAVNLLLNLLLIPALGIAGAGY
                     AKLGSTVTAFLFLFMVLRWQLKISRLLPYARIVLLTAAFLLTLGLMRELNLPLYLLLS
                     VVIFVLFLRAFTIFTYEEREKFTNLITGLLKKRSRRT"
     gene            4242..5348
                     /locus_tag="PKM23_05445"
     CDS             4242..5348
                     /locus_tag="PKM23_05445"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNY90953.1"
                     /translation="MKVNFLDLHAQYLSIKEEIDDAIQQVLARSAFAAGPFVKSFEEH
                     FAAAHQAKCCVGVNSGTAALHIVMMALEIGPGDEVLVPANTFFATPEAVSLNGARPVF
                     VDQEAEYYNIDPEKIEQAITPRTRAIIAVNLYGQPAQLERIKAIADKHHLVLIEDCAQ
                     SHLATLNGKSTGTFGLCGCFSFYPGKNLGAYGEGGAVITDDEALYKKIMMLRDHGSAQ
                     KYHHDLIGHNYRLEGMQGAILDVKLKYLPAWTEKRRHNAALYRKYLRDIPQLVLPAEM
                     PGAKHVYHIFCVRVPRRDEVIKYLGENQIYTNIHYPIPCHLQKAYAGLGYKNGDMPVA
                     EGYADQLLSLPMSEQLKEEEIQYVAEKITAFYQS"
     gene            5390..6469
                     /locus_tag="PKM23_05450"
     CDS             5390..6469
                     /locus_tag="PKM23_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNY90954.1"
                     /translation="MKPFARALLIVASGLMAAGCDKEENPVKSQVTYQADLAVPFAGY
                     SDSVRVCAWKDDKRAAYSVAFDDATRSSHYLVSAPELEARGMRGTFNLNTKGITDWEP
                     WQKLFDKGHELASHSWSHLRMGDLDEIALHREFSLAKSDLLNHIKGLKEVPSFTFPMG
                     SSSALARAVALQYHHSARAGSGVNAAVLTQEELSRVKGVGVYPPYDMSRFEAPVNEAL
                     ERGGWVLIYFHTVSAAEESGDTTIPLSLYRVHLDYVKIRADQFWLATQGEAATYLYAR
                     EATEPQLTTREERILQITMAAPNLRGEEAGSLSVVVRKPEEWRDHAIVVELDGAILTT
                     IAASPSEQFVCDLPVNKTVWLWARK"
     gene            6466..7611
                     /locus_tag="PKM23_05455"
     CDS             6466..7611
                     /locus_tag="PKM23_05455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY90955.1"
                     /translation="MTAGRRIVMLLSNPFEPDVRVLKEARSLSEAGYAVTVLCWDRLG
                     RFPVEEQLEKIHIRRIQIRASYGGGVHLLWSFIRFNLALVSRLLREELEVIHCHDLDT
                     LPAGYIASRLRRKALVYDEHESEYFTQLPALLRGVFRRMEAFLARRAELVLVTNLIQV
                     RKMEALLGRGKAPVEIKNCPPRSFFQPHEGEEEGRLTLGWIGYLQRGTGIDRLVRLFD
                     HLAEGRPDLELLLVGKVHPSFAGELEQLLQASRHRAEIHLMPAVPFDQVYPWYRRLDI
                     AVLLYEDISQYRLNTPTKLFEAMAQGIPVVATPIGDVREIVETHACGLLVGFEDEEEA
                     QRALLRLIEDRALRRRLGDHGYRAAVEHYSWEVMAARLVDHYRKLPS"
     gene            7626..8624
                     /locus_tag="PKM23_05460"
     CDS             7626..8624
                     /locus_tag="PKM23_05460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY90956.1"
                     /translation="MPIQESKFPLVSIVIPMLNESGAIGRCIDSILAQSYPGDRIEIL
                     VVDGLSTDGSRAEVQALAEAHANIRLFDNPQKRTPRSLNIGIRNSRGEVVVILGAHTR
                     IDPEFVACNIRCMREMGVKCTGGTQINTGDSYWQRAIGLGMGSRFGIPSAPYRYDKNE
                     RFVDTVVYAAYARELFDEVGYFDEELHISEDAEMNWRIRKAGYKIYFTPGIISYYYPR
                     PDLRRLARQFFNYGILRVNVIKKHHDAVKPVHLVPPVFVLTTLLLAAAAFRWPLAGRG
                     LLAIWAAYLLYLAVASVSTCREEKQWGYLPALPAVFLTMQIAWGLGFLVGLFKTYK"
     gene            8650..10050
                     /locus_tag="PKM23_05465"
     CDS             8650..10050
                     /locus_tag="PKM23_05465"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNY90957.1"
                     /translation="MSRTLEKILLLLVDFLMINLAFWGLLRLRSSFDLFVEQGAGLQV
                     QIFLLVYLYWLILFVFFGLYQSWYTKSRFDELISVLKTVLLGTFFIFVATFEPERDLA
                     APPTFGRLMIVSYSALMLLCVGGGRLVLHTIQRRLMSSGVGQRNTLIIGYNPQAQKLA
                     DKIHQFPALGYRVIGFLSIKGEEDGQKHGHLHVWGGLNKLRPVVQRRKVEEVILSLGH
                     LPQKRVMHIIGLLEQLPVSIKIEPDLYGLVMGQARTQQIYGFPLIEINPQIMQPWEKT
                     VKRLMDVLFALFFLVLTLPIFVIVALLIKLESPGPVFFKQKRVGKNGAAYTIYKFRTM
                     IQNAEKYTGPVWAGKRDPRITRIGRFLRKTRIDEFPQFINVLAGEMSLVGPRPERPYF
                     VEKLKREYPYYLRRLKVKPGITGWAQVKGDYDTSIEEAREKLAYDLYYIENMSLRMDL
                     RILLYTIAVMLRFKGQ"
     gene            10124..11389
                     /gene="serS"
                     /locus_tag="PKM23_05470"
     CDS             10124..11389
                     /gene="serS"
                     /locus_tag="PKM23_05470"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNY90958.1"
                     /translation="MLDLRFIRENPDLVRKAIQDKGDKADLDRLLALDVTRREIIGAV
                     ENARAQQNKVTQQIAEMKKARQDATAVIAEMRGLSDQIRSLDDKLKGVEEEIYQIQIR
                     IPNIPHESVPVGDPSHNQVVRQWGEIPRKGFKPLPHYDIADRLGLVDFGRAARMSGAS
                     FVSYRGLGARLERALINFMIDLHVSKHGYTEVSPPFVTKRDAMFGTGQLPKLEEDMYY
                     IPGDDLFLIPTAEVPVTNLHREEILEGEELPLLYTAYTPCFRREAGTYGKETRGMVRI
                     HQFDKVEMVKFVKPEGSYDELEKLLANAEEVLQLLKLPYRILCLATGDLSFAAAKCYD
                     IEVWAEGMEKWLEVSSCSNFADFQARRANIRYRAFKGAKPEYVHTLNGSGLALPRTVI
                     AIMENYQTAEGAIIVPEVLRPYMGVEVIR"
     gene            complement(11464..>12307)
                     /locus_tag="PKM23_05475"
     CDS             complement(11464..>12307)
                     /locus_tag="PKM23_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNY90959.1"
                     /translation="NFGSADSPLRYGGMIEVRLPIAEYHNLPLEPFSAGRVGWGFSLL
                     GSRLGDPDDPGAGLNLNANLGLFFHNDHDLVLTTAPDDTLSSAHNSSEFTFGLSATKN
                     LGALGLQAELYGRAFMRKPAGTAYTRENFVYFSPGISYSLPSTIQFHLTSDLRLSGDD
                     DQTRYWHAQAGDLPWKSLPNLPGWRINLGLTVPLIPFPRLGRAARPAAAEEYSRQELE
                     EQLYRQLAGERKKAEEAEESLARIRAERERINAILERLRTTLERSGGQPADSSAAPPA
                     EPAP"
BASE COUNT         2357 a         3759 c         3534 g         2657 t
ORIGIN      
        1 cacctgcaag cataggattg ggcatgccac aaaaagagct gaaaatggtc gatctgctcc
       61 tggtcatcgc caaagcgaaa aaatttattc tgcgcaatgt cttcatcgcc ggtttccttg
      121 ccgttatcat cagcctgttg attccccgct ggttttccgc caaagccacc cttctgccgc
      181 ccaccggaga ctccagcgcc atgggcatct ccgcgctgct gggcaacctg ccggtaagcg
      241 gcttcggcat gggcggattg accgctctca gccaggagac caatctcttt ttggcgatcc
      301 tgaacagccg ttccctgctc ggcgagatcg tcgaaaaatt cgatctgcaa aagcgctaca
      361 agacgaagac gatggaggag accatcgccg cgctgcagga caatatcacc atccaggtca
      421 atgaagacgg gacactgtcg atccgtgcac aggcgaaaac cccctttctg gccgacagcg
      481 cagaggtgat gcagaccaag cgtttggccc gggatatgac caatgccctg gtcgatgagc
      541 tggatgctat caacaaacgc ataaaaaccg atcaggcgcg caacactcgc ctcttcatcg
      601 aaaaacgcta cctgcaaaac cttgaggatc tcaaggcatc ggaggaggcc ctcaagaatt
      661 ttcagcagca gaaccaggcg atttcactgc ccgaccagac ccgcgccatg atcactgcgg
      721 cggcggagct gaaagcccag atcaccgcca aggagatcga agccgatttg atggccaaat
      781 atgtgggcaa aagccacagc gattatatca ggctcgaaaa tgaactgcgg gtattgcggc
      841 ggaaatatga tgagttcaag tacggctccg acgcccaaaa actccccaaa ggcgccctgg
      901 atgagcagga gaaggatctc tttattcccg tggacaaggt gccggatctc ggcctgcgtt
      961 acgtccgtct cttccgcgag gtgaccctgc aggagaagat ccttgaattc ctcctgccgc
     1021 agtatgagca ggcgcggatc caggaggcta aggacacccc gaccgtgcag atcctcgatc
     1081 gcgcggcgct ccccgaacgc aaggcgcgcc cgaagcgggc gctgatcgtg ctgctcgccg
     1141 ttttctttgt tatggttttg agcgcaaccc atgccctgct cagggaacgg ctcgagaccc
     1201 tgcgcgcgac cgaaacccgg cgctatgaag agctgcaatc cttctggaag agtctgcgga
     1261 gcgactggcg cttctggaaa aggtagttcg gcacccccgt gcgttccgga tctgaaatat
     1321 ccgccctcga ggccagaccc tccccggcgg cctcagcgat catcgcactg accgggctgg
     1381 ctctggcgct gacggtcacg cgctggcccc tgctccaggc gctggttgcc gttttcaccc
     1441 ttttggtggt gacgctgctc tttctgcgcc cggagtggtc actggtgctt tttttctcct
     1501 ccgggttgat caaggagtgg ctgctgctga atgtgcccct attcgcccgc ttcgatttca
     1561 cggtcgcgat ctttctgctc agtgcgacaa gcctcttctt ctttctgctc cgccggggtt
     1621 atctcttcga gtttaaaatc caccccagca tgctcccgct ttttcttttc accgcactgc
     1681 tgattttttc catcacttac accccctcgt tcaaatacgg cagcgccaag gccttcagct
     1741 ttttgctttt caactgggcc cttttcctct tccctatcct tctggtccgc gaggaagcgg
     1801 cggcgaagcg gatcatggtc atcctgggca ccctcgccat ttccgcgaca gtctacaccc
     1861 tcttttccct ggtcaagagc ctcttttccg gttcgattat cttttcctac cgggcctcat
     1921 ttcttggcat caatccgatc agttttgcca actggatcgg cgccatttcg ctgctgttga
     1981 tcacctatct gcccagcatc ggcaagaggt ggcatcgtcg tgccgtgatt ctggcgctgg
     2041 gggtgctgct gctggccctg ctggcagcca attcacgcgg cccgatgttc agtttcatca
     2101 tcaccctgat actccttctc gccctgcgtg cccgcaagat ccccgggcgg aagctgatgc
     2161 agtgggtcgc ccttggtctg ctttttgtgg tcatcgccct gcttgtgctg cccgaacagg
     2221 tgaccagccg gtataccgac gtcattaccc aggaggaagg gaccaggaac tataccttct
     2281 tcactgtaaa tacgcgcctg ttcgcctgga atgccgctct ggatatggcc actgcgcgca
     2341 tctcctccat cctgatggtt gtcggcagcg gggggttcag tcaggtggtt tacggacagg
     2401 atgtccgcct ctatccccac aatatattca tcgaggtttt ttgcgagctg gggattgcgg
     2461 gcgttgtggt gctgttgtgg tatctggcca gcatcctcgg cgatgtcctg cagaccctgc
     2521 gccggaagat gaccgaacgg atgcgctctc ttttcatggc cttttgcatg gccgccctat
     2581 tcaattttgt caacgcccag ttcagcggtg atctcaatga caaccggcgg ctgtggtttt
     2641 tcctcggcat ggtcatcgcc ctgatgcagg ttattcgcag cagctctgtg gaagcggatc
     2701 atggaagtat ccgatagccg cgtcgtcagc aactccctgt ttatctacgc cgggcgcgtt
     2761 atcaacctgg ttttcaattt tttcatcttc gtctacctcg ccaactatct gggtgagagc
     2821 gcctttggcc gcttctccat tgccatcacc tacgttggca ccttcgatat catcgccaat
     2881 ttcggtctga atcagatcct tgtgcgcgag ctggcagggg gcaggacaga aaaaagccgg
     2941 ctgctgggca acggcatcct cctcaagggg gtgatcaccc tgctggcact ggcttgcgcc
     3001 ctggccatcc tccccttcat ggcctacccg cgcgagacca tggtcgtggt ctggatcatc
     3061 ctcgtcaacc tgatcgtctc ctccaagctc tcttccaccc gcactgtctt cgaaagcatt
     3121 ttccaggcgc agctgcgcat ggcctttccc attctctgca atatccttga taatctcctc
     3181 tttgtcctgc tcatttttct ggcggcccat ttcttcaacg ccggactgac cggcatggcc
     3241 ctgatctacg gcctctgcaa cctgcccggg acgttctggc tggtgggcag atttctgcgc
     3301 accaaccagg tctcctggca gccgcagtgg ccggtcctgc gcgagctgct gcgcgagtcc
     3361 ttgccgctcg cattctatct ctttttttcc attctgacga ctaaaatcga tgtgctgatg
     3421 ctctcctgga tgaggaatga ggccgaggtg gggcaatacg cagccgccac ccggctggtc
     3481 tatcccctca gctttctctc gacctcgctg acaatatcgc tttttccgct gctctccaaa
     3541 tcctttcatg aacgcaggga ggagtttgac cgcctctgcc ggcgcggcag caaatatgtt
     3601 tttctcatcg gtcttttcac cagtggggtg ctggctttcg ccgccgatcc gatcatccgt
     3661 acgctctatg tgccgggcta tgcggcctgt attcctgcgt tccgccttct gctgatttcg
     3721 cttggattca gctttctcaa tttctatttc atcgatattc tcatctcggc ccggcgccag
     3781 aagctggtca ccggggtgat ggccctggct ttcgcagtca atctgctgct caatctcttg
     3841 ctcattcccg ccctgggcat cgccggggcc ggctacgcca agctgggcag cacggtgacc
     3901 gcctttctct tcctctttat ggtgctccgc tggcagctga aaatcagccg gctgctgccc
     3961 tatgcccgca tcgtgctgct gacagcggcc ttcctcctga ccctgggatt gatgcgggag
     4021 ctgaatctgc cgctttatct cctcctttcg gttgtcatat ttgtcctctt cctgagggcc
     4081 tttacaattt ttacgtatga agaaagagaa aaatttacta atttaataac tggattactc
     4141 aaaaagcgca gcaggcggac ctgacagttt tcggccatac caaccccttg agggtgcgat
     4201 gacaggcctg cccttacacc caggacagag gagcaggagc gatgaaagtc aattttttgg
     4261 atttgcacgc gcaatatctc tcgatcaaag aggagatcga tgacgccatc cagcaggtgt
     4321 tggccaggag cgccttcgct gcgggtccct tcgtcaaatc cttcgaggag catttcgccg
     4381 ccgcccacca ggccaaatgc tgcgttggtg tcaactcggg tacggcggcg ctgcacatcg
     4441 tgatgatggc tcttgagatc ggccccgggg atgaggttct cgtgccggcc aatacctttt
     4501 ttgccacgcc ggaggcggtc agcctgaacg gcgccagacc cgtctttgtc gatcaggaag
     4561 cggagtatta caacatcgat cccgaaaaaa tcgagcaggc catcactcct cgcacccggg
     4621 cgatcattgc agtcaacctc tatgggcagc ccgcccagct cgaacggatc aaggcgatcg
     4681 ccgataaaca tcatttggtg ctcatcgagg actgcgccca gtcgcatctc gccactctca
     4741 atggcaagtc gacggggacc ttcggcctct gcggatgctt cagcttttat cccggcaaga
     4801 acctcggcgc ctatggcgag ggaggcgctg tcatcaccga tgatgaagcc ctctataaaa
     4861 agatcatgat gctccgcgat cacggctccg cccaaaagta tcaccatgac ctgatcgggc
     4921 ataattaccg gctcgagggc atgcagggag ccatcctcga tgtcaaactg aagtatctgc
     4981 cggcatggac ggagaaacgc cgtcacaatg cggcgctcta ccgaaaatac ctcagggata
     5041 ttccgcagct cgtcctcccc gcggagatgc cgggcgccaa acatgtctac catatctttt
     5101 gcgtgcgtgt gccgcgccgc gacgaggtga tcaagtatct gggcgaaaac cagatctaca
     5161 ccaatattca ttaccccatc ccctgccacc tgcaaaaagc ctatgccggg ctgggctata
     5221 aaaatggcga tatgcccgtg gcggagggct acgcggacca gctcctctcg ctgccgatgt
     5281 ccgagcagct caaggaagag gagatccagt acgtcgccga aaaaatcacc gcgttttacc
     5341 agagctgaaa gctgctgcca gtaactgctc atttatgatc aaggagatga tgaaaccgtt
     5401 tgcccgtgcc ctcctcatcg tcgcgtcagg cctgatggcc gctggctgtg acaaggaaga
     5461 gaatccggtc aaaagccagg taacctatca ggcggatctg gcggttccct ttgccggata
     5521 cagcgattcc gtgcgcgttt gcgcctggaa ggatgacaag cgagccgcct actcggtcgc
     5581 tttcgatgat gccacccggt cgagtcatta tctggtctcc gccccggagc tggaagccag
     5641 agggatgcgc ggaaccttta atctcaacac caagggtata acggattggg aaccctggca
     5701 gaagctcttc gacaaggggc atgagctggc ctcgcacagc tggtcgcatt tgcgcatggg
     5761 cgacctcgat gagatcgcct tgcatcgtga attcagcctg gccaagagtg atctgctcaa
     5821 ccacatcaag ggtctcaagg aggtgccaag tttcaccttt cccatgggca gctcctctgc
     5881 tttggcccgg gccgtcgccc tgcagtatca tcacagcgcg cgcgccggat cgggggtcaa
     5941 tgcggcagtt ttaacgcagg aggaactctc ccgcgtcaag ggggttgggg tctatcctcc
     6001 ctatgacatg agccgctttg aagccccggt caacgaggcc ctggaaagag gtgggtgggt
     6061 gctgatctac tttcataccg tcagcgccgc ggaagagagc ggggatacca ctattccgct
     6121 ctcactctat cgggtgcatc tcgactatgt caagatccgg gcggaccagt tctggctggc
     6181 cacccagggc gaggcggcga cctatctcta cgcccgcgaa gccacggaac ctcagctgac
     6241 gacgcgagag gagcgcatcc tccagatcac catggctgct cccaacctgc gcggcgagga
     6301 ggctggttcg ctttcggtcg tggtccgaaa gccggaggag tggcgggatc atgccattgt
     6361 cgtcgaactg gatggggcga tcctcaccac cattgcagcg tcgccctcag agcagtttgt
     6421 ttgcgacctg ccggtcaaca agacggtctg gttatgggcg cgcaaatgac cgctggccgg
     6481 cggatcgtga tgctgctctc caatcccttt gaaccggatg tgcgcgtgct caaggaggcg
     6541 cgctccctga gcgaagccgg atatgcggtg acggtgctct gctgggaccg gctcggccgg
     6601 tttcccgttg aagagcagtt ggaaaaaatc cacatccggc gtattcagat ccgtgccagt
     6661 tacggtggcg gggttcacct gctgtggagt ttcatccgct tcaacctggc cctggtcagc
     6721 cgcctgctgc gggaggagct ggaggtcatt cactgccatg atctcgatac cctgccggca
     6781 gggtatatcg cctcgcgcct gcggcgcaag gccctcgtct atgacgagca cgagagcgag
     6841 tatttcaccc aactgcccgc tctgctgcgc ggagtgtttc ggcgcatgga ggcctttctc
     6901 gcccgacgcg ccgaactggt cctggtcaca aacctgatcc aggtccgcaa gatggaggcc
     6961 cttctcggcc ggggcaaggc accggtcgag atcaagaatt gcccgccgcg ctctttcttc
     7021 caaccccatg agggtgagga ggaggggcgc ttgaccctgg gctggatcgg ttatctccag
     7081 cgcgggaccg gtatcgatcg tctcgttcgc ctctttgacc atttggccga ggggcggccc
     7141 gacctggagc ttttactggt cggcaaggtg catccctcct ttgccggtga gctggagcag
     7201 ctgctccagg cgagccgcca ccgggcagag attcatctca tgccggcggt gcccttcgat
     7261 caggtctacc cctggtaccg ccggcttgac attgcggtgc tgctctacga ggatataagc
     7321 cagtaccggc tgaacacccc caccaagctc ttcgaggcca tggcgcaggg cattccggtg
     7381 gtcgctaccc cgatcggcga tgtccgggag atcgtcgaga ctcatgcctg tggcctcctc
     7441 gtcggctttg aggatgagga agaggcgcaa agagccctgc tgcgcctgat cgaggatcgg
     7501 gccctgcgcc ggcggctggg cgatcacggc tacagggctg ccgttgagca ctacagctgg
     7561 gaggtcatgg ccgccaggct ggtcgatcac tacagaaaac tgccaagtta gtacaggacg
     7621 ctgttttgcc tatccaggaa tcaaaatttc ccctggtctc cattgtcata ccgatgctca
     7681 acgaatcggg ggcgatcgga cgctgcattg actccatcct tgcccagagc tatcccggcg
     7741 atcgcatcga gatcctcgtc gtcgacgggc tctcgacgga tggctcgcgc gcggaggtgc
     7801 aggccctggc tgaagcgcat gcgaatatcc gcctcttcga caatccgcaa aaacggacgc
     7861 cgcgcagcct caatatcggc atccgtaaca gcaggggcga ggtggtggtc atcctcgggg
     7921 cgcacacgcg catcgatccg gaatttgtcg cctgcaacat ccgctgcatg cgcgagatgg
     7981 gggtcaaatg caccggcggc acccagatca ataccggcga cagctactgg cagcgtgcga
     8041 tcggactcgg catgggctcg cgcttcggca tcccgagcgc accctaccgc tatgacaaga
     8101 acgaacgctt cgtcgatacg gtggtttatg ctgcctacgc ccgcgagctc ttcgacgagg
     8161 tgggctattt cgacgaggag ctgcacatct ccgaggatgc ggagatgaac tggcggatcc
     8221 gcaaggcggg gtacaagatt tatttcacgc ccgggatcat ctcgtactat tatccccgtc
     8281 ccgaccttcg ccggctggcg cggcagtttt tcaattatgg catcctgcgc gtcaatgtga
     8341 tcaaaaagca ccacgatgcg gtcaaacccg tccatctcgt gccgcccgtt ttcgtcctga
     8401 ccaccctgct gctcgcggcg gcggcgttca ggtggccact ggccggccgc gggctgctcg
     8461 ccatctgggc cgcctatctc ctctacctgg cagtcgcctc ggtcagtacc tgccgggagg
     8521 aaaaacagtg gggttatctg ccggccctgc cggcggtctt tctcaccatg cagatcgcct
     8581 ggggtctggg gttcctggtc ggactcttca agacctacaa atgagcatac agcgcataag
     8641 gatcgttcca tgagccggac gctcgaaaaa atcctcctgc tgctggttga ttttctgatg
     8701 atcaacctcg ccttctgggg actgctgcgc ctgcgcagct cgtttgacct cttcgtcgag
     8761 cagggggccg ggctgcaggt gcagattttc ctccttgttt acctctactg gctgatcctg
     8821 tttgtctttt tcgggctcta tcagtcctgg tacaccaagt cccgctttga tgagctcatc
     8881 tcggtgctca agacggtcct tctcggcact tttttcattt tcgtggccac cttcgagccc
     8941 gaacgcgatc tggccgcacc gcccaccttc ggccgcctga tgattgtcag ctattcggcc
     9001 ctgatgcttc tctgcgtcgg gggcggacgc ctggttttgc ataccatcca gcgccggctg
     9061 atgagttcgg gggtgggaca gcgcaatacc ctgatcatcg gctataatcc ccaggcgcaa
     9121 aagctcgccg acaagatcca ccagtttccc gcactgggct atcgtgtcat cggctttctc
     9181 agcatcaagg gcgaagagga cggccagaaa catggccatt tgcatgtctg gggcggactc
     9241 aacaaactgc ggccggtggt gcagcggcgc aaggtggagg aggtgattct ctccctgggc
     9301 catcttccgc agaaacgggt catgcacatc atcggcctcc tggaacagct gccggtgagc
     9361 atcaagatcg agcccgatct ctacggcctg gtcatgggcc aggcgcgaac ccagcagatc
     9421 tacggatttc ccctcatcga aatcaatccg cagatcatgc agccctggga gaaaacggtg
     9481 aagcggctca tggatgtcct ctttgcgctc ttttttctcg tcctgaccct gcccattttc
     9541 gtcatcgtcg ccctgctcat caagctcgag agccccggcc cggtcttttt caaacagaag
     9601 cgggtcggca agaacggcgc cgcctataca atttacaaat tccgcaccat gatccagaat
     9661 gcggagaaat ataccggacc ggtctgggcg ggcaagcgcg acccgcgcat cacccggatc
     9721 ggccgcttcc tgcgcaagac gcgcatcgac gagtttccgc agttcatcaa tgtcctcgcc
     9781 ggggagatga gcctggtcgg cccccgcccg gaacgcccct acttcgtcga gaaactcaaa
     9841 cgcgaatatc cctattacct gcgccgcctc aaggtcaaac ccgggatcac cggctgggcc
     9901 caggtgaagg gggattacga taccagcatc gaggaggcca gggaaaagct cgcttatgac
     9961 ctctactata tcgagaacat gtccctacgt atggacctgc gcatcctgct ctataccatc
    10021 gccgtgatgc tgcgcttcaa gggacagtga ggaacgattc ggagaaggcc ggcctctctt
    10081 caccacggct tttgcaagga ctttatcaat tgaggtatga attatgcttg acctgagatt
    10141 tatccgtgaa aatcccgatc tcgtccgcaa ggccattcag gacaaggggg acaaggccga
    10201 tctggaccgc ctgctcgccc tggacgtgac ccggcgggag attatcggcg ccgtcgaaaa
    10261 tgcgcgtgcg cagcagaaca aggtgacgca gcagattgcg gagatgaaga aagcccggca
    10321 ggatgccaca gccgttatcg ccgagatgcg cgggctcagc gaccagatca ggagtctgga
    10381 cgacaagctc aagggggtcg aggaggagat ctaccagatc cagatccgca tccccaacat
    10441 cccccacgag agcgtgccgg tcggagaccc gagccataac caggtggtgc ggcagtgggg
    10501 cgagatcccc cgaaagggct tcaaacccct tccccattat gatatcgccg ataggctggg
    10561 actggtcgat ttcggccgcg ccgcccgcat gagcggcgcc tccttcgtca gctatcgcgg
    10621 cctcggcgcc cggctcgagc gcgccctgat caatttcatg atcgatctgc acgtctccaa
    10681 acacggctat accgaggtct cgccgccctt tgtcaccaag cgcgacgcga tgttcggcac
    10741 cggccagctc cccaagctgg aagaggatat gtattacatc cccggcgacg atctctttct
    10801 catcccgacc gccgaggtgc cggtgaccaa ccttcaccga gaggagattc tcgagggtga
    10861 agagctgccc ctgctctata ccgcctatac cccctgtttc cgccgcgagg cgggaaccta
    10921 cggcaaggag acccgcggca tggtgcgcat ccaccagttc gacaaggtgg agatggtgaa
    10981 attcgtcaag cccgaaggct cctacgatga gctggaaaag ctgctcgcca atgccgagga
    11041 ggtactgcag ctgctcaagc tgccctaccg catcctctgc ctggcgacgg gcgacctcag
    11101 cttcgccgcg gccaagtgct acgacatcga ggtctgggcc gaggggatgg aaaagtggct
    11161 cgaggtttcg agctgcagca actttgccga tttccaggcc cgccgcgcca acatccgcta
    11221 ccgcgccttc aagggcgcca aacccgaata tgtgcacacc ctcaacggct cgggactggc
    11281 cctgccgcgg acggtgatcg ccatcatgga gaactatcag acagcggagg gcgccatcat
    11341 tgtgcccgag gtgctccgtc cctatatggg ggtggaggtc atccgctgac caggacgcca
    11401 acaaaaaaag ccgcttcttc gcatgaggaa gcggcttttt ttattgcggg gagaaaagag
    11461 ggatcagggc gcgggctcgg cggggggggc ggcgctgctg tcggccggct ggccgccgga
    11521 tcgctccagg gtagtgcgca gtctctccag aatcgcgttg atgcgctcgc gttcggcgcg
    11581 gatgcgcgcc aggctctcct ccgcctcttc cgcttttttg cgttcccccg ccagctgccg
    11641 gtagagttgt tcttccagct cctgccggga atactcttcc gcggctgccg gtcgggcggc
    11701 ccggccgagc cggggaaagg ggatcagggg caccgtcaac ccgaggttga tccgccaccc
    11761 agggaggttg ggcagcgatt tccagggcaa gtcgccggcc tgagcatgcc agtagcgggt
    11821 ttgatcgtca tccccggaga gccggagatc gctggtgaga tgaaactgga tggtggaggg
    11881 gaggctgtag ctgatccccg gtgaaaaata gacgaaattc tcgcgggtat aggccgtacc
    11941 cgccggcttg cgcatgaagg cgcggccgta gagttcggcc tgcaggccga gggcgccgag
    12001 gtttttggtg gccgagaggc cgaaggtgaa ctcggagctg ttgtgcgccg atgagagggt
    12061 gtcatccggg gcggttgtaa gcaccagatc gtgatcgttg tggaagaaaa ggccgagatt
    12121 ggcgttgaga ttcaggccgg cgccggggtc atcgggatcg ccgagccggc tgcccaggag
    12181 ggaaaatccc cagccgacgc ggccggcgga gaagggttcg agcggcaggt tgtggtattc
    12241 ggcgatgggc aggcgtacct cgatcatgcc cccgtagcgc agaggggagt cggcgctgcc
    12301 gaaattg
//
LOCUS       DAOPWU010000070        12270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5264_length_12270_cov_5.435039, whole genome shotgun sequence.
ACCESSION   DAOPWU010000070 DAOPWU010000000
VERSION     DAOPWU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5264_length_12270_cov_5.435039"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKM23_05480"
     CDS             complement(<1..301)
                     /locus_tag="PKM23_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNY90960.1"
                     /translation="MDQTMLPDGQVEARVSAVDRGSYRILHNGRELPAELSGRFSFLA
                     DSPLDLPGVGDRVSVQLYDSDSAAIIHGLQERRSFLRRRRAGQTVEYQLIAANIDI"
     gene            501..1127
                     /locus_tag="PKM23_05485"
     CDS             501..1127
                     /locus_tag="PKM23_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90961.1"
                     /translation="MKRSGVGVVAIMVGIVPALLAQSSTWYSQEEAERRLAHIQEIAA
                     ASNGRIEITVIRVPGEGGVRAHGPSAEPRGEPLTAGAQSGLMPATAEAVGLPRQPAAD
                     SLTALEASDLPWGGEFTPPENPDLTWQQQYDHALRLEKYGSLHRALRILLQLTYSHPD
                     HPRCGECYYQMGECLTALGQRPLAQRAYRQVLRYPECAWYGDALQKVK"
     gene            1354..2358
                     /locus_tag="PKM23_05490"
     CDS             1354..2358
                     /locus_tag="PKM23_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNY90962.1"
                     /translation="MNVTIREVARTAGVAVSTVSLVLNRKGRVSRETERKVLDAIEKL
                     HYHPQRSARGLVTRHSGNLGFILSTDHFSNAEPFYTKIFLGTEFEARSHDYYVLLTTI
                     EQQFSEKSIPRFLLEKNVDGVILAGTVPPKILECIKERGLPCVLIDYFPPAGNYSAIL
                     IDNADGIRQAVTHLIEKGHRDLVFVGGDSQHPGMRERRDAFRRILAEHDLDADERRIC
                     ADSLASGAQEGYEAALHFLNNGVKLTAFVAANDAIASGILRACRDRKLRIPEDVAITG
                     FDDVETAITARPTLTTLHVPKEEMGALAVRLLAQLIAGSASAGTKIVVPVQLVPREST
                     "
     gene            2619..5837
                     /locus_tag="PKM23_05495"
     CDS             2619..5837
                     /locus_tag="PKM23_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNY90963.1"
                     /translation="MNHCKHALWGVLMLCLGLALQPALAQINTIYNGGFENAELPANW
                     NKVDANDAEVVWTSDQYRSPLRSLKISESGGADAPAWQSSNMAKLEWNPTTGIPANIE
                     MVIGGWVKTENVNTSPSSPAAEITLSFTFYDKSKNMIFGQPVTMAVPQAQASSGWTEI
                     KNSAPVLLPSDADSLIITFAFGANATGTVWLDDLFMKVAPGASGWLGDLYNANFGVPA
                     GWFFWKDRMSEGEQDYGTVSITDKYAHRGSYSLLIADEAGNPAEVVAISDRNQVEPGR
                     SYGFSAWVKLEGANLNPKRDIEQAIFFTITYHSGEAGWAEVGGEDFVVIDQSRSTTDW
                     RLYSFTFRVPDNASRISVRARMQHQAVGETYWDDIRIYPLGIAKANLNFDESVQPANW
                     SGYAHGDAAVEWASDAWRSAERSLKISKTAGTADPLWISGNMAKLEWNPTTGIPPSIE
                     IVVGGFVKTQNVNINPANAAQEVQLAFSFFDKDKNMIFGQPVVLKVPQAQAAIDWTEI
                     KNDAPIVLPTDADSLVISFSMGAEATGTVWLDDIFMKLAPGASGWIGDLYNANFGVPA
                     GWFFWKGGMSEGIGDEKGIVTITDKYAHSGTYSLRVADNAANGDEVVAISDRNPVLPD
                     TDYLISAWVKLEGATVNTTKDVEKSILFTVTYHSDEAGWAERHGADFFVVDQSAADRD
                     WALYSFRIRTEPLDRRISIRARLQHQTTGDVYWDDFQVMPIAMAGDGMGFESDLPAYW
                     TKINEGGSTLAWTGDVARSPQRSLMIQKGAGNETPAWQTHANMAKLQWNPTTGIPPSI
                     EIVVGGWVKTENVNVNPATADGEIHLVYTFYDKSKAVIFGQPVVLKVPQALATSDWTE
                     IKNDAPIVLPTDADSMSIQFAFGAAATGTVWLDDIFMKLAPGASGWIGDLYNANFGVP
                     SGWFFWKDHMSEGEIDYGTVSITRDAAHSGDYSLLISDDETNPAEVVAISDRNPILPG
                     RLYTVQAWVKTEGVTVNTTQDVEKSVFFTLTYHTGEEGWAEVGGTDFFVVDQSVTDKD
                     WTLYNFTVAPPANATRMSIRRVCSIRPPARSTGMISLWSRAT"
     gene            5819..6151
                     /locus_tag="PKM23_05500"
     CDS             5819..6151
                     /locus_tag="PKM23_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY90964.1"
                     /translation="MVEGNVTRVPGQGNSAAPAVYRLAQNYPNPFNPETRITFSIPQD
                     SRVQLAVYDLLGNPVRTLISAKMAAGEHQVIWDGRDDSGRMASSGIYFYILESKDARM
                     MRKMTLLK"
     gene            6210..8924
                     /locus_tag="PKM23_05505"
     CDS             6210..8924
                     /locus_tag="PKM23_05505"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY90965.1"
                     /translation="MRLLPVYWLLAASCFLAAPLQAGTTGKIVGRVADAGSRESLPGV
                     NVIVEGTSLGAVTDLEGQYLILRVPPGSYTLRAKLLGYTDMSYDRVQISIDKTTTVDF
                     SLSQTVLQTGQTITVTASKPLVERDLTSTVATVNSEMIGRLPVDNMQDVINLQAGVVD
                     GHFRGGRSGEVGYLIDGISINDVYSGSSAVQVENTAVQELQVISGTFNAEYGQAMSGI
                     VNVVTKEGGENYHGSLTSYVGDYVSSHTDIFWNVDAFNPIYNLEGTLSGPVPVTRNRL
                     TFFASGRFYDNEGALYGRRVFLPSDSANFPSSDVRDWYIESHGTGRRFASQEDFSRYA
                     DSLKQGADFAAMRPSRRWTGQIKLAWKLSAANKLEYEGLVQKQRYREYDHQFRYNPDG
                     DFWRHQTGWNNGLTWTSVLNQRNFFTVKMARFYTDYKQYVYEDPYDARYVNPKLLDAA
                     SGNAFLSGGQQMWHFLRNTTTSVGKVDFTSQATNEHQLKAGLEYRQHRMWLRAFEIRL
                     NRDTQWKPYVPAVTKNVQNNDEYLLHPLEVSAYVQDKIELAYMVVNAGVRYDYFKSDG
                     IVPIDFLQPAASPLRTPKTSQQISPRFGLSYPVTDRGAIHISYGHFFQIPNFEFLFSN
                     PDFELYATSDYGSTSPPERVPNTIGNAELKPQKTTIYEIGLQQQLGADLSLEVTAYFK
                     DIRNLLGTEILHTTTGIRYARYINRDYGGVKGLTFAFEKRLTGGFGATVDYTYQIARG
                     DASDPASAFLDAEAGREPQKQLVPLNWDRTHSLNLSLTAGDARVWSASLIGRLGSGLP
                     YTPTQQNVRTAVENSERKPAFMNFDFYAYRNFRVGEQNLNAFVRVYNLLDQKNELDVY
                     SDTGRANYTLSALASGTIFGVNSLSDYLARPNFYSDPRQVLAGIGLEF"
     gene            8948..12103
                     /locus_tag="PKM23_05510"
     CDS             8948..12103
                     /locus_tag="PKM23_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90966.1"
                     /translation="MSRLKIVCCLLLAWSLPLLWAQRKPDPNVGALANTRWGTMDGNV
                     VRTVFANHGEIANWDEPAWPSGEWPKGSGHTYVDGVALIVATPIVDIHGKRYHDVVTR
                     YREDMSTSPEGIPWGFAPLPGYFNPDVKTNIHNYPAMSNDPTTWPLTWPDQDAEWNGQ
                     WNGFFGRGKTNADLEAYFVFDDDPDEKYLYYPDPSDSSRRGLGIEVASRLFQWNQVLA
                     QDCIFSIYFITNEGKRDYDSTYFAFHIDWGIGGHDDSADDAGSYDLDLDIAWAFDGNG
                     YGSPNNWAPVGVAGFAFLESPGIFRDSRDNDNDGLINERRDSGPGVFLNAYPYGIDDI
                     RAFQNFYTDREIRPHWSGDENINWDPYSDLNQNGQRDEGEPLNDDLGADGLGSNDGNY
                     TGPDEGEGDGIPTPGEPDFDYLDKDESDQIGLTGFQVFVLHEYKMEYDEAYWNGLKSA
                     PPPREKLVQNTNLGMFFSSGPFGLKAGDTQFYSMSLLFGEDQNQLARAKKTVQQIYNA
                     TYQFARPPDKSRLTAVPGDGRVVLYWDDKAEKSWDPFLQEHDFEGYRIYRTTDAEFSE
                     AQVITDAYGKARFRKYIAEFDLANGIKGLHPIDIEGIKFDLGEDTGLRHYYVDTNVDN
                     GQTYYYAVVPYDRGLATVSSTGEISGISPSEASSVIRVSAAGVVEYVDINCAVVTPRA
                     PSAGYQPPQLAGGIEHQGPGTGHIEVELLDADALQDNATYEVIFHDTSAFALNRQAAF
                     DCINTTSQTALLEGEQLEEGLGITPVVEGMVVHVSGDTACQVIEAETGWVKGAHSWGF
                     RVGKNPSLSGRFVPYPADFEISFYDHIVDTSLALLFGQTATPVHFMIYNATEARTMDF
                     LFGDKDKDGLFSPGDSVTIVVGLRLGEPLPTPRSKFKTAWTFFYDQAAGGSDRPASGD
                     IFRARTSKPFRDGETFRFTIQGARESVSQASERLSGITVVPNPYCAAASWEPRNPFRF
                     GRGERRIFFNHLPARCTIRIYTVRGYLVNTIEHDSSLDDGSESWDLVSKDGMDIAYGV
                     YLFQVDAPGVGTHIDKFAVIK"
BASE COUNT         2497 a         3988 c         3463 g         2322 t
ORIGIN      
        1 cgatatcgat attggcggcg atgagctggt actccacggt ctggccggcg cggcggcggc
       61 gcaggaagct cctccgctcc tgcaggccgt gaatgatggc ggctgaatcg ctgtcgtaga
      121 gctgaacact gacccgatca ccgacgccgg gaaggtcgag cggcgagtcc gccagaaacg
      181 aaaaacggcc ggagagttcg gcggggagct cgcgtccgtt gtggagaatc ctgtaggagc
      241 cgcgatcgac agcagagacg cgcgcttcca cttgtccatc cggcagcatg gtttgatcca
      301 ttggggaatc ctttccgatg tgatgacttc atcttacaaa gcctgcgcat aaaaagcaag
      361 caattcccgc atcagtcagc gcccaaaaag atgaccccga tcacaagagc tgcacgagag
      421 tgtacgcagt caaatggttt tgtccctata ttataataca gcagagaaga cagcagggcg
      481 ggcaggaagg gcagcggacg atgaagcgga gcggtgttgg tgtggtggcc atcatggtgg
      541 ggattgtgcc cgccctgctg gcacaatcct ccacgtggta ctcgcaggag gaggctgagc
      601 gcaggctggc gcatatccag gaaattgcgg cggccagcaa cggcaggata gagatcaccg
      661 tcatccgcgt gcctggtgaa ggcggggtga gagcgcacgg cccctcggcc gagccgcgcg
      721 gagagccact caccgccggg gcgcagtccg gtttaatgcc ggccactgct gaggccgtag
      781 gattgcctcg gcagccggct gcagacagtc tgactgcact ggaagcatcg gaccttccct
      841 ggggcggcga gttcaccccc cctgaaaacc ccgatctcac ctggcagcag cagtacgacc
      901 acgcccttag acttgagaaa tatggatcat tgcaccgcgc cctgaggatt ctgctgcagc
      961 tcacctattc ccatcccgac catccccgct gcggggagtg ctattaccag atgggcgaat
     1021 gcctcaccgc cctcgggcag cgtcccctcg cgcaacgggc ctatcgtcag gtgctgcgct
     1081 atcccgagtg cgcctggtac ggcgatgcct tgcagaaggt gaagtagccg cttcttgcag
     1141 gaagcagcct ttaactgttc agaattcccc tcgtggtatt ttccccgggt ttcccgattc
     1201 aagaaaatta aaaatagtca atttttctct tgacataagt ttgaaaatag gctatctttc
     1261 agtgtcgaaa cgtttcgaca ataatcatta tttaagaggc taaatctatt ataataaatt
     1321 gatataacta attttataaa ataaagcgtt tcgatgaatg taaccattcg agaagtcgcc
     1381 cggactgcgg gagtagcggt ttccaccgtc tccctggtcc tcaaccgcaa gggacgcgtc
     1441 agccgcgaaa ccgagcgcaa ggtcctcgat gccatcgaaa aactccacta tcacccccag
     1501 cgctccgccc gcggcctggt cacacggcac agcggcaacc tcggctttat cctcagcacc
     1561 gaccactttt ccaacgcaga gcccttttac accaagatct ttctcggcac ggaattcgaa
     1621 gcccggtcgc acgattatta tgtgctcctg accaccatcg agcagcagtt ttcggagaaa
     1681 agcatccccc gtttcctgct ggaaaaaaac gtggacgggg tcatcctcgc cggcaccgtc
     1741 ccccccaaaa tcctcgaatg catcaaggag cgcggcctgc cctgcgttct catcgactac
     1801 tttccgccgg cgggcaacta ttcggccatc ctgatcgaca acgcggatgg catccgccag
     1861 gcggtcaccc atctcatcga aaagggccac cgcgatctgg tctttgtcgg cggagacagc
     1921 cagcatcccg gcatgcgcga gcggcgggat gcatttcgcc ggatcctggc cgagcatgac
     1981 ctcgacgccg atgaaaggag aatctgcgcc gactccctcg ccagcggcgc ccaggagggc
     2041 tatgaggccg ccctccattt cctcaacaac ggggttaaac tgaccgcctt cgtcgccgcc
     2101 aacgatgcca tcgccagcgg catcctgcgc gcctgccgcg accgcaagct gcgcatcccc
     2161 gaggatgtcg ccatcaccgg ttttgatgat gtcgagaccg ccatcaccgc ccgtcccacc
     2221 ctgaccaccc tccatgtccc caaggaggag atgggcgctt tggcggtccg cctgctggcc
     2281 cagctcatcg ccggatcggc gtcagcgggc accaagatcg ttgtaccggt ccagcttgtc
     2341 ccccgtgaat cgacctgatc caggcagctc gaaacagaac ggcacttctc gccatcacca
     2401 gcccggtacc agagtatcgc aattaccgct tggttttacg tccggagtca cgacatagca
     2461 atgagaggag gtgagatcgt catctctgtc aaaacgccat gacggagcag gctgagtgcc
     2521 caatttcata cactcttatc ggatggaacg gccacgggct tgcctctttg gcgcatgcat
     2581 gcatcgctgt tcactaccat caacatggag gagtagtcat gaaccattgc aaacatgccc
     2641 tgtggggtgt attgatgctc tgcctgggtc tggccctgca gccggccctg gcccagatca
     2701 atacaattta caacggcggc tttgaaaacg ccgaactccc cgccaactgg aacaaggtcg
     2761 atgccaacga tgcggaggtc gtttggacat ctgatcaata ccgctccccg ctgcgctcgc
     2821 tcaaaatctc ggagagcggc ggcgccgatg ccccggcctg gcagtcgagc aacatggcca
     2881 aactggagtg gaaccccacc accggcattc ccgccaacat cgagatggtc atcggcggct
     2941 gggtgaaaac cgaaaatgtc aacacctccc cctcttcccc cgctgccgaa atcaccctca
     3001 gtttcacctt ttacgacaag tccaaaaaca tgatcttcgg ccagcccgtc accatggcgg
     3061 tgccacaggc gcaagccagc tccgggtgga cggagatcaa gaacagcgcc ccggtcctcc
     3121 tgccgtcgga tgccgactcg ctgatcatca ccttcgcttt cggcgccaat gccaccggca
     3181 cggtctggct ggatgacctc ttcatgaagg tggcgcccgg ggccagcggc tggctcgggg
     3241 atctttacaa cgccaatttc ggcgtgccgg cgggctggtt cttctggaag gaccgcatga
     3301 gtgagggcga acaggactac ggcaccgtct ccatcaccga caaatatgcc caccgcggca
     3361 gctattccct gttgattgca gacgaagccg gaaatcccgc cgaggtggtc gccatctccg
     3421 accgcaacca ggtcgaaccc ggccgctcct atggtttctc cgcctgggtc aagctggagg
     3481 gagccaacct caatcccaaa cgggatatcg agcaggcgat ctttttcacc atcacctacc
     3541 acagcggcga agcgggatgg gcggaagtgg gtggagagga ttttgtcgtc atcgatcagt
     3601 ccaggtcaac gacggactgg cggctctact ccttcacctt ccgggtgccc gacaacgcct
     3661 cgcgcatttc ggtacgcgcg cgcatgcagc atcaggccgt cggcgagacc tattgggatg
     3721 atatccgcat ttatcccctc ggcattgcca aggccaatct caatttcgat gaatcggtgc
     3781 agcccgccaa ctggtccggc tatgcgcatg gcgacgctgc cgtcgagtgg gcctccgatg
     3841 cctggcgttc ggccgagcgc tccctgaaga tcagcaagac ggccggtacc gccgatccct
     3901 tgtggatctc cggcaacatg gccaagctgg aatggaaccc gaccacgggc atcccgccca
     3961 gcatcgagat cgtggtcggc ggctttgtca agacccagaa tgtcaacatc aatccggcga
     4021 atgcagctca ggaggttcag ctcgctttct cctttttcga caaggacaag aacatgatct
     4081 tcggccagcc ggtcgtgctc aaggtcccgc aagcgcaggc ggccatcgac tggaccgaga
     4141 tcaagaacga tgcccctatc gtcctgccca ccgatgccga cagcctggtc atcagtttca
     4201 gcatgggagc ggaggccacc ggtacggtct ggctggatga cattttcatg aagctggcgc
     4261 ccggggcgag cggctggatc ggggatctct acaacgccaa ctttggcgtc ccggcgggct
     4321 ggttcttctg gaagggaggc atgtcggagg gcatcgggga tgaaaagggc atcgtcacca
     4381 tcaccgacaa atacgcccac agcggcacct actccctgcg cgtcgccgac aatgccgcca
     4441 acggggatga ggtggtggcg atctccgacc gcaatcccgt cctgcccgac accgactatc
     4501 tgatctccgc ctgggtcaag ctggaggggg ccacagtcaa caccaccaag gatgtcgaaa
     4561 aatcgatcct cttcactgtc acctatcatt ccgacgaggc cggctgggcc gagcggcacg
     4621 gggcggactt tttcgtcgtc gaccagagcg cagccgaccg cgactgggcg ctctacagct
     4681 tccgcatccg gaccgagccg ctggaccggc gcatctccat ccgcgcccgc ttgcagcatc
     4741 agaccaccgg cgatgtttac tgggatgatt ttcaggtcat gccgatcgcc atggccgggg
     4801 atggcatggg tttcgagtcc gacctgcccg cctactggac caagatcaat gagggcggct
     4861 cgaccctggc ctggaccggc gatgtcgcgc gttcgcccca gcgcagcctg atgatccaga
     4921 agggtgcggg caacgaaacc ccggcctggc agacccatgc caacatggcc aaactgcagt
     4981 ggaatccgac cacaggcatc ccgcccagta ttgaaatcgt ggtcggcggc tgggtcaaga
     5041 ccgaaaatgt caatgtcaat cccgccactg ccgatggcga gatccacctc gtctatacat
     5101 tttacgacaa gagcaaggcg gtgatcttcg gccaaccggt cgttctcaag gtcccccaag
     5161 ctctggcaac cagcgactgg accgaaatca agaacgacgc tcccatcgtc ctcccgaccg
     5221 acgccgactc gatgagcatc cagttcgcct ttggcgctgc tgctaccggc acggtctggc
     5281 tggatgatat attcatgaag ctggcccccg gtgcgagcgg ctggatcggg gatctttaca
     5341 atgccaattt tggcgtcccg tccgggtggt tcttctggaa ggaccatatg agtgagggcg
     5401 agatagatta cggaacggtg agcatcaccc gcgacgccgc ccacagcggg gattactccc
     5461 tgctcatctc ggacgacgaa accaatccgg ccgaggtggt ggcaatcagc gaccgcaatc
     5521 cgatcctgcc cggacgtctc tacaccgtgc aggcctgggt gaaaaccgag ggtgtaacgg
     5581 ttaataccac gcaggatgtc gaaaaatcgg tctttttcac cctgacctat cacaccggtg
     5641 aggagggctg ggcggaggtc ggcggcaccg atttctttgt cgtcgaccag agcgtgacgg
     5701 acaaggactg gacgctctac aatttcaccg ttgcgcctcc agccaacgcc acgcgcatgt
     5761 ccatccggcg cgtctgcagc atcaggccac cggcaaggtc tactgggatg atttcgctgt
     5821 ggtcgagggc aacgtgaccc gggttcccgg ccagggcaat tctgctgcgc cggcggtcta
     5881 tcgtctggcc cagaactatc ccaatccctt caatcccgag acgcggatca ccttctccat
     5941 cccgcaggac agtcgggtcc agctggcggt ctatgatctg ctcggcaatc ccgtccgcac
     6001 cctgatctcg gccaaaatgg ccgcaggtga acaccaggtc atctgggatg gacgggacga
     6061 cagcggccgg atggcctcct ccggcatcta tttctatatc ctggagagca aagacgcacg
     6121 catgatgcgc aagatgaccc tgctcaagta aaaccgcgcg tgccacgcat ccgtccgggt
     6181 gcgtggcacg cagactttgg gagttaccca tgcgactttt acctgtttat tggctgctgg
     6241 ccgcgagctg ttttctggcc gcgccgctgc aggccgggac caccggcaaa atcgtcggcc
     6301 gcgtcgcgga cgccgggtcc agggaaagtt tgcccggcgt caatgtcatc gtcgagggga
     6361 cgagcctcgg cgccgtcacc gacctggagg ggcagtatct catcctgcgg gtgccaccgg
     6421 gcagctatac cctgcgcgcc aaactgctcg gctataccga catgagctac gaccgggtgc
     6481 agatctcgat cgacaagacc accaccgtcg atttcagtct ctcgcagacc gtgctgcaga
     6541 ccggtcagac catcaccgtc accgcctcca agcccctggt cgaacgcgac ctcacctcca
     6601 ccgtggccac ggtcaattcc gagatgatcg gccgcctgcc ggtcgacaac atgcaggacg
     6661 tcatcaacct gcaggccggc gtggtcgacg gccactttcg cggcggccgc agcggcgagg
     6721 tcggttacct catcgatggc atctccatca acgatgtcta ttccggcagc tccgccgtgc
     6781 aggtagagaa taccgcagtg caggagctgc aggtgatcag cggcaccttc aacgccgagt
     6841 atggccaggc gatgtccggc atcgtcaacg tggtcaccaa ggagggcggc gaaaactatc
     6901 acggctcgct caccagctac gtcggggatt acgtcagctc gcataccgac atcttctgga
     6961 acgtcgatgc cttcaacccg atctacaatc tcgagggcac cctgagcggg ccggtgccgg
     7021 tcacccgcaa ccgcctcacc tttttcgcct cggggcgttt ttatgacaac gaaggcgccc
     7081 tctacgggcg gcgggtcttc ctcccctccg attcagccaa ctttcccagc agtgatgtgc
     7141 gcgactggta catcgagtcc cacggcacag gccgccgctt tgcctcgcag gaggatttca
     7201 gccgctacgc cgacagcctc aagcaaggcg ccgatttcgc cgccatgcgt ccgagccggc
     7261 gctggaccgg acaaatcaag ctcgcctgga agctctccgc tgccaacaag ctcgaatacg
     7321 aggggctggt gcagaagcag cgctaccggg aatacgatca ccagttccgc tacaatccgg
     7381 acggcgattt ctggcgccac cagacgggct ggaacaatgg cttgacctgg acctcggtcc
     7441 tgaaccagcg caatttcttc accgtcaaga tggcgcgctt ttacaccgac tacaagcagt
     7501 atgtctatga ggatccttac gatgcgcgct atgtcaatcc caagctgctc gatgcggcca
     7561 gcggcaacgc ctttctcagc ggcggtcagc agatgtggca tttcctgcgc aacaccacca
     7621 ccagcgtcgg caaggtcgat ttcacctcac aggcgaccaa tgagcatcag ctcaaggccg
     7681 gcctggagta tcgccagcac cgcatgtggc tgcgcgcctt cgagatccgc ctcaaccgcg
     7741 acacccagtg gaagccctac gttcccgcag tgacgaagaa tgttcagaac aacgatgaat
     7801 atctcctcca ccctctcgag gtttctgcgt acgtacagga caagattgaa ctggcttaca
     7861 tggtcgtcaa tgcaggggtg cgctacgatt atttcaagtc ggatggcatc gtgccgatcg
     7921 attttctcca gcctgcggcc tctccgctgc gcacgcccaa aacgagccag cagatcagcc
     7981 cgcgcttcgg cctctcctat cccgtcaccg accgcggggc gattcacatc tcctatggcc
     8041 atttcttcca gatccccaat ttcgagttcc ttttctccaa tcccgatttc gagctctacg
     8101 ccacctccga ttatgggagc acctcgcccc ccgagcgggt gcccaacacc atcggcaacg
     8161 ccgaactcaa gccgcaaaag accaccatct acgaaatcgg cctgcagcag cagctgggcg
     8221 cggatctttc cctggaggtg accgcctatt tcaaggatat ccgcaatctc ctcggcacgg
     8281 agatcctcca taccacgacc ggcatccgtt atgcgcgcta tatcaaccgg gattacggcg
     8341 gggtcaaggg cttgaccttc gcctttgaga agcggctgac gggcgggttc ggagcgacgg
     8401 tcgattatac ctatcagatc gcgcgtgggg atgcctccga tccggcctcc gcttttctcg
     8461 atgcggaagc ggggcgcgaa ccgcagaagc agctggtccc cctcaactgg gaccgcaccc
     8521 acagcctcaa tctttcgctc acggccggcg acgccagggt ctggtccgcc agcctgatcg
     8581 gccgtctcgg cagcggtctg ccctataccc cgacgcagca gaatgtgcgc acggcggtgg
     8641 agaacagcga gcgcaagccc gcttttatga atttcgattt ttacgcctat cgcaatttca
     8701 gggtgggcga gcagaatctc aatgcctttg tcagggtcta caatctcctc gatcagaaga
     8761 acgagctgga tgtctacagc gacaccggcc gcgccaacta taccctgtcg gcgctcgcct
     8821 cgggcacgat tttcggcgtc aacagcctct ccgactatct ggcccggccc aacttttatt
     8881 ccgatccgcg gcaggtcctg gcgggcatcg ggctggaatt ctgaaacaaa acaagagagt
     8941 gtcgattatg agtcgcctga aaatagtctg ctgcctgctt ttggcctggt ccctgcccct
     9001 gctctgggct cagcgcaagc ccgacccgaa tgtcggcgcc ctggccaata cgcgctgggg
     9061 caccatggac ggcaacgtgg tgcgcacggt tttcgccaac catggcgaga tcgccaactg
     9121 ggatgagccg gcctggcctt ccggtgagtg gcccaaggga agcggccaca cctacgtcga
     9181 cggcgtcgcc ctcatcgtcg cgacccccat cgtcgatatc cacggcaagc gctatcacga
     9241 cgtcgtcacc cgctatcgcg aggacatgtc gacttcgccg gaggggatcc cctgggggtt
     9301 cgccccgctg ccgggctatt tcaatcccga cgtcaagacc aacatccata actatccggc
     9361 gatgagcaac gatccgacca cctggccgct cacctggccc gatcaggatg cggagtggaa
     9421 cgggcagtgg aacggctttt tcggccgggg caagaccaac gccgatctcg aggcctattt
     9481 cgtcttcgat gatgatccgg atgaaaagta tctctactat cccgatccct cggactcgtc
     9541 ccgccgcggg ctgggcatcg aggtcgcctc ccgcctcttt cagtggaatc aggtgctcgc
     9601 ccaggactgc attttttcca tctatttcat caccaacgag ggcaaaaggg attatgacag
     9661 cacctatttc gcctttcata tcgactgggg catcggcggc cacgatgatt ccgccgacga
     9721 tgccggcagc tacgacctcg acctcgacat cgcctgggct ttcgacggca acggctacgg
     9781 cagtcccaac aactgggcgc cggtcggcgt cgccggcttc gcctttctcg aaagccccgg
     9841 catcttcagg gacagccgcg acaacgacaa cgacggtctg atcaacgagc gacgcgacag
     9901 cgggcccggg gtttttctta atgcctatcc ctacggcatc gatgacatca gggcctttca
     9961 aaacttttat accgatcgtg agatcaggcc gcactggtcc ggtgacgaga acatcaactg
    10021 ggatccctac agcgatctga accagaacgg gcagcgggac gaaggcgagc ccctgaatga
    10081 tgatctcggc gccgacggcc tcggcagcaa cgacggcaac tataccggac ctgatgaggg
    10141 cgaaggggac ggcatcccga cccccggcga acccgatttc gattacctcg acaaggatga
    10201 atcggaccag atcggcctca ccggctttca ggtcttcgtc ctccacgaat ataaaatgga
    10261 gtacgacgaa gcctactgga acggcctgaa atcggcgccc ccgccgcgtg aaaaactggt
    10321 gcagaacacc aatctgggca tgtttttcag ctccggcccc ttcggcctga aggcgggcga
    10381 tacccagttc tactccatgt ccctcctctt cggtgaggat caaaaccagc tcgcccgggc
    10441 gaaaaagacc gttcagcaga tctataacgc cacctatcag ttcgcccggc cgcccgacaa
    10501 atcgcgcctg accgccgtgc ccggcgacgg cagggttgtg ctctactggg acgacaaggc
    10561 ggaaaaatcc tgggacccct tcctgcagga gcatgatttc gagggctacc gcatctaccg
    10621 caccactgat gccgagttct ccgaggccca ggtcatcacc gacgcctacg gcaaggcccg
    10681 cttccgcaag tatatcgccg aatttgatct cgccaacggc atcaaggggc tccaccccat
    10741 cgacatcgag gggatcaaat tcgacctcgg cgaggacacc ggtctgcgcc actattatgt
    10801 cgataccaat gtcgacaacg gccagaccta ttactatgcc gtggtcccct atgaccgggg
    10861 gctggcgacg gtctcgtcga ccggcgagat ctccggcatc tccccctccg aggcctccag
    10921 cgtcatccgc gtcagtgccg ccggggtggt ggagtatgtc gacatcaact gcgccgtggt
    10981 caccccgcgc gctccctcgg cgggctacca gccgccccaa ctggcgggcg gcatcgagca
    11041 ccagggcccc ggcaccggtc atatcgaggt tgagctgctc gacgccgatg ccctgcagga
    11101 taacgccacc tatgaggtga tctttcatga cacctcggcc ttcgcgctca accgtcaggc
    11161 ggccttcgac tgcatcaata ccacttccca gaccgcactg ctggaagggg agcagctgga
    11221 ggagggcctc ggcatcacac cggtggtcga aggcatggtg gttcatgtca gcggcgacac
    11281 cgcctgccag gtcatcgagg ccgagaccgg ctgggtcaag ggcgctcaca gctggggctt
    11341 ccgcgtgggc aagaatccca gcctgagcgg acgtttcgtc ccctaccccg ccgatttcga
    11401 gatcagcttt tatgatcaca tcgtcgacac ctcgctcgca ctgctcttcg gccagacggc
    11461 gacgccggtg cactttatga tctacaatgc caccgaggcg cggacaatgg actttctttt
    11521 cggcgacaag gacaaggacg gcctcttctc gccgggggac tcggtcacca tcgtcgtcgg
    11581 gctcaggctg ggtgaaccgc tgcccacgcc cagatcgaaa ttcaagaccg cctggacctt
    11641 cttctacgac caggctgcgg ggggcagtga ccggccggct tccggcgata tcttccgcgc
    11701 gcggaccagc aagcccttcc gcgatggcga aaccttccgt ttcaccattc agggcgcgcg
    11761 cgaaagcgtc agccaggcca gcgagagact ctccgggatc accgtggtcc ccaatcccta
    11821 ctgcgccgcc gccagctggg agccgcgcaa tcccttccgc ttcggccgtg gcgagcgccg
    11881 catcttcttc aaccacctgc ccgcccgctg caccatccgc atctatactg tgcggggcta
    11941 tctggtcaat accatcgagc acgacagcag cctcgacgat ggctccgaga gctgggatct
    12001 ggtttccaag gacggaatgg acatcgccta cggcgtctac ctcttccagg tcgatgcccc
    12061 cggtgtcggc acccatatcg acaaattcgc ggtcatcaag tgacctgccg ttgaacattt
    12121 tacggactcg cttatgaaaa agatcgtcat tgtcactctc ctgctgctga cggccgcggg
    12181 gcttcgcgcc ggggatgtcg ccaaggtcgg cgtcaccgcc gcccccttcc tctccatcgg
    12241 cgtcggcgcc agggcgaccg gcatgggcgg
//
LOCUS       DAOPWU010000071        12225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5289_length_12225_cov_5.271241, whole genome shotgun sequence.
ACCESSION   DAOPWU010000071 DAOPWU010000000
VERSION     DAOPWU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5289_length_12225_cov_5.271241"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PKM23_05515"
     CDS             complement(<1..387)
                     /locus_tag="PKM23_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90967.1"
                     /translation="MKKMIRVLPGLLLIWAHAVLAAAQMPYRCTVVESSDKALVYDIV
                     FDSVSVAQKLSGGQWITDVTLEGATYTDQYNLPRLPVTSVLFGVPAAGQVRLTLLAEE
                     SATRSSGALRLADKPVYSVVDSEGRER"
     gene            complement(430..711)
                     /locus_tag="PKM23_05520"
     CDS             complement(430..711)
                     /locus_tag="PKM23_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90968.1"
                     /translation="MHDAEQNKAPTGANPPPLSERQRRLGHELRSAAQGLLGYINIFS
                     DEMQSRLTPEEAVLMERIWHYGKKLSELSMELLNELQELSQRLSDREPE"
     gene            complement(704..1951)
                     /locus_tag="PKM23_05525"
     CDS             complement(704..1951)
                     /locus_tag="PKM23_05525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNY90969.1"
                     /translation="MSGLRKNRRTQLFFMILAGAFIARFLLIARLDQRVVYSDEQKNL
                     LLAANLTGGAGYVLADGKPTAVIPAGYPLWLAALRMAGLTGPAQVRLAQLVISLATIV
                     FVAFFSRMLFGSSAALLAAAGCALYPYFIFLPGTILATTLYSMLLVLGAWMYMEAVNT
                     NNNKMMFFGGMVWGWAVLTVTTAIVLAGATVVWHLRHASGSRGEKLRLAGCFLAGMLL
                     LLAPWMVRNQHKLGRPLLATNGGYNLWLGNNPAADIEAPCGVPTPPAMDERIIRSGSE
                     LYADSLFSATARAYIAAEPGAFAKRTLLKGLFFWRLDPSPVTASYLSRGELVRILGLI
                     TFLPLLILTFWGYIKAPPQLKKRMALFFYYALAYTAVHAVMIIKVRLRLPLDHFIIMM
                     AAYGLTLVRPPRPEPEERMPGSA"
     gene            complement(1982..3007)
                     /locus_tag="PKM23_05530"
     CDS             complement(1982..3007)
                     /locus_tag="PKM23_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90970.1"
                     /translation="MRKVVPLFQAALVLLMGCAGCEKDPVTPKVEEKGWRALPLQVTD
                     RKVQCIAVHPDHPATLYVGLFDGLYKSTDGGSNWHSITTGLNSRDIKSVEIVADNPQR
                     LYCGSTGFGISRSDDGGESWTNLKGEVANTLVNDIHLVNQRDETIWLATATGIYRKGG
                     GEEKWTNTFLNSRLIHAVTSLPGNPATLLAGLLYTGFARTTDAGARWYYANSGVEASG
                     TYYDSPVQFAYIGADSGLICAVTVNGDFYTSQDAGQSWKYAYGAQGQGRGVAVVTHPR
                     LRDRLYLANTTSVFRSTDRGATWSKMSLEMPAGVAITAIQVANGDPGVVYIGTEEHGL
                     YSYTERH"
     gene            complement(3018..4313)
                     /locus_tag="PKM23_05535"
     CDS             complement(3018..4313)
                     /locus_tag="PKM23_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY90971.1"
                     /translation="MKESAGRILIVVQNLPVPLDRRVWLEATTLRDNGYQVSVICPTS
                     REYPKVHEVIDNIQIHRYRIPFEARGVLGYAAEFIYAWLQTARLSLRVLRREGFDVLQ
                     ACNPPDTYFLLGLLYKLVGKEFIFDHHDLSPEMFSAKFRGRRGLLYHALLLLEKLTLK
                     TARVVLVTNESYRDIALERGHKKAEDVFVVRTGPDLSRLHPVAPAPELRRDRPFLVCY
                     LGEMCQQDGVDYLLRSAHYLHRTLHRHDVTFVLIGGGPAVEDLKRMSAEMGMTGYLHF
                     TGRISDDELARYLSTADVCVDPDPWSEWANNSTMNKILEYMVFARPIVAFDLKEIHYS
                     ARRAALYARPNDVRHFAQKINVLLDNPQMRADMGAYGRQRVINELAWVHTHPPLLAAY
                     ARVFNRPQPLPHLAEARTALALSLRKFELGGLNCNQVIR"
     gene            complement(4294..5610)
                     /locus_tag="PKM23_05540"
     CDS             complement(4294..5610)
                     /locus_tag="PKM23_05540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNY90972.1"
                     /translation="MKISIFGLGYVGCVSAACLAEMGHDVIGVDINAHKVRLINEGLS
                     PIVEEGLEELILAQRQAGRLRATTAPDLSDRDLVFICVGTPSNGNGSLHLDYVNRVCT
                     DIGRLLRSAGHRITVVLRSTVLPGVAEEMAIPALEAASGMHAGSDFGFALNPEFLREG
                     SSVHDFYHPPKTVIGVFDQESADLLSALYSSLPAPLFQLAPGEASMIKYADNAFHAVK
                     VAFANEIGRMCKNFQVDSRVVMNVFTQDTKLNISPLYLKPGFAFGGSCLPKDLRALTH
                     RARQDDVAIPMLNSALESNEEHLQHALRLVKACGRKRIGVLGLSFKHGTDDLRESPVV
                     ALVEELVGKGYEVRIYDRNVSMARLMGANKEYIEREVPHIAKLMCSSMEELLARSEVV
                     VIGNNGQEHERVLGDLRNGHKIIDLSGLKNGRHTHIKEVNYEGICW"
     gene            complement(5640..6599)
                     /locus_tag="PKM23_05545"
     CDS             complement(5640..6599)
                     /locus_tag="PKM23_05545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNY90973.1"
                     /translation="MNSRPLHLSVVIPARDRLDVLRRVLDGLCKQELAPEHFEVIVVD
                     DGSSDGTMDHLREQAGKTPFTLISLQGGGEGAAAARNRALAASRGEIILFLDADTIPS
                     PSLLRRHLELHSRARGAECHLGRIEMSAELMGPKQARWHELRLAAGDARTGEIDFRRY
                     RTANSSMPRSALLEIHGFEERLPAAEDLELAYRLARRGMRFFYHSDTLAVHHHPLRLE
                     EYLRKGALYGEAVARWHALQPEMGTELARRFGLYDAGLTPAERRRYRLKTLLVNGASV
                     RPLTVLAGCSRRIGCAASQRLLRAIYGYHLRRSFYGSRLQPGV"
     gene            complement(6596..8035)
                     /locus_tag="PKM23_05550"
     CDS             complement(6596..8035)
                     /locus_tag="PKM23_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNY90974.1"
                     /translation="MLQTLIKNTVWRSLADVVARLGSALFWILLARYLGAGTFGAISF
                     ALALMGFFELVSSLGLSSVLTRDAAQNPATAAAYFGHMLIIGLASSAAGGVLMAGAAW
                     LIRPDPGTLRIVILLAFLLPFTCLAYWSRAMLTAAEKMQFISMGTLAENAILILLGLG
                     LLLAGRGIMAVVAALALSKVAAAVLLYHLAGSRVARPIWRLERGMTAYLLQQVPLFLS
                     IAFFNALFWSITVVLITWLEGETAAGYYSAAYKLISYVLLFAVAFSQALFPVAARLAR
                     RDRALYSRLLRRALHYLLMLFIGAALVLSLLAEPVILFLYGPAMAPAIPVLRWLAWMV
                     VPYGVIPVLAYTLVSHHRQGRDLMANFLAAATVVIVNLLLIPRFSAIGGAAAMVAGSA
                     VFAVVELGSVHTLLYPLRPDRRTLALLASAALLALTLLFSQGMNFFLRAAAGGSVYLT
                     ALWLLQAIDRSDLRQLWRAAWPQCREEMA"
     gene            complement(8016..8867)
                     /locus_tag="PKM23_05555"
     CDS             complement(8016..8867)
                     /locus_tag="PKM23_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY90975.1"
                     /translation="MNSKQISRLIGRSLWRRRAGYALIHLTTLREWYIARAMRRLLAA
                     RGDMSSALDAGCGMGQHTYALARRAPALRIEAVEQDAEQAADLADFFQRLGLERVQVR
                     AGDITRDDLGGAVDLILCCSVLEHIPDDLALLQRFHALLRERGSLLIYVPLSERRVLK
                     SLERRIAAMTRAAGGELPHGHVRYYTPELLASRLGQCGFEVTRRELSYGPCGRLAYDL
                     VTAVQFSSLFLVLFPFYLVLVHPFVLLLMAIDYFSVNRDGNGLLIVAGKSGGITVEET
                     ADAANPD"
     gene            complement(8869..10368)
                     /locus_tag="PKM23_05560"
     CDS             complement(8869..10368)
                     /locus_tag="PKM23_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNY90976.1"
                     /translation="MNSYSVNNEERSILRQIINLLILAAIAVGVSSAAWFIWGLRAGW
                     SMYAFIGLLMPFVLAIVPDFRRFVMWWMLFLIPLGLDYKFFYQRSISGHSGIAIGTTE
                     ILLFILLVQWLVTAVHERNRERIQWFPAITLPTLALIIMSSFSILAARNITLSLFDIA
                     MYGKMLIFFLYLANNIRDNRDVGLVVTALLFGLVMESGVMVAQYYSGSALGLVGTEEL
                     SNFVAYKREARMVLRPGGTVGNVNGFARYLGFILPIASILMLTTRERKMFLLSLLAAM
                     GGLVALILTQSRSVWGAFLVAMMAGMVFILMRNLVTLRTLKRIGMALLLVAGLAFFYG
                     ETVYNRLTGDDHGSAKSRLTTARVALEIINDHPVLGVGVNNYDSYIREYWRIEDPFTK
                     VAVVHNNYLLILAEIGLIGFAAFLWLLAALLVRTMTAMKCRVKFFREIAVGLFVSVVC
                     FLLASLADGYKSSLTLMYLFWTIAAITEALIHLDRSWRDQAVELAGKKS"
     gene            complement(10408..11382)
                     /locus_tag="PKM23_05565"
     CDS             complement(10408..11382)
                     /locus_tag="PKM23_05565"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNY90977.1"
                     /translation="MSAIFDELEKIDAKEGIAAPPWPAGEESGQTGILPCSLPEEILS
                     DFYDLREYIRIAGQRSQMRVLTIASSISGEGSSTIATLLAFLLSGGMEKGLAERVQAE
                     AAGSDFIAGSELALADAPEQDAPAESASTSEDAVQEQRAGEEIFQSRFTDYIQKEAAE
                     ALIQAIKKGGVLLVDANLYNPALHRFFGIEQDEGLAEIIEEKRDWRKLTRCLSQGDLH
                     IITAGAAKGKPADIVGSEELRELVKSWREEFRYVIIDAPAVLNYVDALSLSALSDGVI
                     LVVRAGQTRWEVAQNAKRKLSVAQANLLGVALNRQKITIPDGFYNRLV"
     gene            complement(11422..>12225)
                     /locus_tag="PKM23_05570"
     CDS             complement(11422..>12225)
                     /locus_tag="PKM23_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNY90978.1"
                     /translation="KGEDLGDLLIELYTFLIREYQQGRFSILIIDEAQNLKPEVLEEI
                     RQLSNLETASQKLLQVILSGQPQLDRYLNDPALVQLKQRISLKATLHRLNEADTSAYI
                     RHRLKVAGARKQEIFTEAALQVIFEVSDGIPRLINQVCDNALMAAAAQKVRQIDAALI
                     LDLVQEDKVMTAEAGPAGDPGQAEKSENAAPEGESWVPPAGSAAAAAEIIERAAEICS
                     APATGEAAAPADEQSEPLPARRRPRVEPAVELEFAAGFDGLDLGELSIF"
BASE COUNT         2472 a         3740 c         3896 g         2117 t
ORIGIN      
        1 tcgctcccgc ccttcggaat cgacgacgct gtagaccggc ttgtccgcaa gccgcagggc
       61 gcccgagctg cgcgttgcgc tctcctcggc aagaagggta aggcggacct gccccgcagc
      121 gggcaccccg aagagcacgc tggtcaccgg cagacgggga agattgtact ggtcggtata
      181 ggtcgcgccc tcgagggtga catcggtgat ccactggccg ccgctcagct tttgggcgac
      241 gctgaccgaa tcaaaaacaa tatcatagac cagcgccttg tcggacgact ccacgacggt
      301 gcagcggtag ggcatctgcg cagcagccag cactgcgtgc gcccatatca gcagcaggcc
      361 ggggagcacc cggatcatct tcttcatgcc gtttcctttc tctctccgca tcctgtcggt
      421 cctcttgctc tattccggtt cccggtctga gagccgctgg ctgagctcct gcagctcgtt
      481 cagcagctcc atgctcaact cggataattt cttgccatag tgccagatgc gctccatcag
      541 caccgcctcc tcgggggtca ggcgcgactg catctcatcg ctaaagatgt tgatgtagcc
      601 gaggaggccc tgggcggcgg aacgcagctc atgacccagc cggcgctgcc gctcggaaag
      661 cggcggcggg tttgcgccgg tgggcgcttt gttttgctcg gcgtcatgca ctgccaggca
      721 tcctctcttc gggttcaggc cgcggcggcc ggacgagagt caggccgtag gcggccatca
      781 tgataataaa gtggtccagg ggcagtcgca gccggacctt gatgatcatc acggcgtgca
      841 ccgccgtgta ggccagggcg tagtagaaaa agagggccat cctcttcttc aactgcggcg
      901 gagccttgat atatccccaa aaggtcaaaa tcagcagggg cagaaaggtg atcagcccca
      961 ggatccggac cagttccccc ctgctgagat aggaggcggt caccggcgag ggatcaaggc
     1021 gccagaaaaa cagccccttg agcagggttc gcttcgcaaa ggctccgggc tcggctgcga
     1081 tgtaggcgcg ggctgtcgcg ctgaagagcg agtcggcata gagctccgat cccgaacgga
     1141 tgatgcgctc atccattgcc gggggtgtgg gcacgccgca gggggcctcg atatcagcgg
     1201 ccggattgtt gccgagccag agattataac cgccgttcgt cgccagcagc ggcctgccca
     1261 gcttgtgctg gttgcgcacc atccaggggg ccaggagcag aagcataccg gcgagaaagc
     1321 atccggccag cctgagcttc tcccccctgc tgccggaggc gtgacgtaaa tgccacacca
     1381 ctgtggcgcc ggcaaggaca atcgcggtgg tgacggtgag aaccgcccag ccccagacca
     1441 ttccgccaaa gaacatcatc ttgttattat tggtatttac agcttccatg tacatccagg
     1501 cgccgagtac caggagcata ctgtaaaggg tagtggctaa aatcgtgcca ggcaggaaga
     1561 tgaaataggg atagagagcg cagcctgcag cggcgagcag cgccgcggag gatccgaaga
     1621 gcatgcggct gaaaaaggcg acgaaaacga tggtggccag gctgatcacg agctgggcga
     1681 ggcggacctg cgcgggcccg gtcagaccgg ccatgcgcag ggcggcgagc cagaggggat
     1741 aaccggccgg gatgacggcc gtcggcttgc catcggcaag cacatagccc gcacccccgg
     1801 tcaggttggc ggcgaggagc aggtttttct gctcgtcgct gtagacgacg cgctggtcga
     1861 gacgggcgat gagcaaaaag cgggcgataa aggcgcccgc cagtatcatg aagaagagct
     1921 gggtgcggcg gtttttccgc aatccggaca tggtcggcaa atcgttctcc tctgcggctg
     1981 actagtgtct ctcggtataa ctgtaaagtc cgtgctcctc agtcccgata taaaccacgc
     2041 cggggtcgcc gttggcgacc tggatggcag tgatagcgac gccagccggc atttccagcg
     2101 acatcttgct ccaggtggcg ccgcggtcgg tgctgcgaaa gaccgaggtg gtattggcca
     2161 ggtagagccg gtctcgcagc cgtggatggg tgacgacagc cacgcccctc ccctgtccct
     2221 gcgcaccata ggcgtacttc cagctctgcc cggcatcctg cgaggtgtag aaatcgccgt
     2281 tgacggtgac agcgcagatc aggccggagt cggccccgat ataggcgaac tggaccggac
     2341 tgtcgtaata ggttccgctg gcctccaccc cgctgttggc gtaataccac cgtgctccag
     2401 cgtcggtcgt gcgcgcaaag ccggtgtaga gcagtcccgc caacagggtc gccggattgc
     2461 ccggcaggct ggtgacggcg tggatcaggc ggctgttgag gaaggtgttg gtccattttt
     2521 cctcgccgcc ccccttgcgg tagatgccgg tcgcagttgc gagccagatg gtctcatcgc
     2581 gctgattgac gaggtggata tcattgacca gggtgttggc cacctcaccc ttgaggttgg
     2641 tccagctctc cccaccgtcg tcgctgcggc tgatgccgaa tcccgtactg ccgcagtaaa
     2701 ggcgctgggg attgtcggcg acgatctcca ctgatttgat atcacggctg ttcagcccgg
     2761 tggtgatgga atgccagttg gagccgccat ctgtgctctt gtaaaggccg tcgaagaggc
     2821 cgacatagag agtggcgggg tggtcgggat ggacggcgat gcactgcacc ttgcggtccg
     2881 tcacctgcag aggaagcgca cgccagccct tttcttcgac tttcggcgtg acgggatctt
     2941 tttcgcagcc ggcgcacccc atcaggagca cgagtgcggc ctgaaacagc gggacaacct
     3001 tgcgcatctt cttctcctta cctgattacc tgattgcaat tgagaccgcc cagctcgaac
     3061 ttgcgcagcg aaagggcgag agcagtcctg gcctcggcca gatgcgggag cggctgcggc
     3121 cggttgaaaa cccgggcgta agcggccaga agcggcggat gggtgtggac ccaggccagt
     3181 tcgttgatca cccgctgccg cccgtaggcg cccatatcag cgcgcatctg cggattgtcg
     3241 agcaaaacat tgattttctg ggcaaaatga cggacatcat tgggccgtgc atagagagcg
     3301 gcgcgacggg ccgagtagtg aatctccttg agatcaaagg cgacgatcgg cctggcgaag
     3361 accatatatt cgagtatctt gttcatggtg gagttgttgg cccactccga ccagggatcg
     3421 gggtcgacgc agacatctgc ggtggagaga tagcgggcca gttcgtcgtc actgatccgg
     3481 ccggtgaaat ggagataacc ggtcatcccc atctccgcgc tcatccgctt cagatcctcg
     3541 acggccgggc cgccgccgat cagaacgaaa gtgacatcat gccgatgcag ggtgcggtga
     3601 agatagtggg ccgagcgcag cagataatcg accccgtcct gctggcacat ctctccgaga
     3661 tagcagacca ggaaggggcg atcgcgccgc agctccgggg cgggcgcaac cgggtgtagc
     3721 cggctgaggt ccggacccgt gcgcacgaca aagacatcct ccgccttttt atggccgcgc
     3781 tccagggcga tgtcgcggta ggactcgttg gtgaccagga ccacccgggc ggtcttgagg
     3841 gtcaattttt ccaggaggag cagggcatgg tagagcagcc cgcgccggcc ccgaaacttg
     3901 gctgagaaca tctcgggtga aagatcgtga tgatcaaaga tgaactcctt gccgaccagc
     3961 ttgtagagca ggccgaggag gaaataggtg tcgggcggat tgcaggcctg cagaacgtca
     4021 aaaccttccc ggcgcagcac ccgcagcgaa aggcgtgctg tctgcagcca ggcgtagatg
     4081 aactcggctg cgtagcccag cacacctctc gcctcaaagg ggatgcggta gcggtggatc
     4141 tgaatgttgt cgatgacttc atgcaccttc gggtactccc gggaggtggg gcagatcacc
     4201 gaaacctggt agccgttatc gcgcagggtg gtcgcctcga gccagacccg gcggtcaagc
     4261 ggcaccggca ggttctggac gacgatcaaa atcctaccag cagattcctt cataattgac
     4321 ctctttgata tgagtgtgcc tgccgttttt cagtcccgac aggtcgatga ttttatggcc
     4381 gttgcgcaga tcgcccagca ccctctcgtg ctcctggcca ttattgccga tgacgacgac
     4441 ttcggaacgg gcgagcagtt cttccatgga actgcacatc agcttggcga tatggggcac
     4501 ctcgcgttca atgtactcct tgttggcgcc catgagccgc gccatcgaga cattgcggtc
     4561 gtagatgcgc acctcgtagc ccttgcccac cagttcctcg accagggcca cgaccgggct
     4621 ttcgcgcaga tcgtcggtgc cgtgtttgaa gctcaacccg agcacaccaa tgcgcttgcg
     4681 gccgcaggcc ttgacgagcc ggagggcgtg ctggagatgc tcctcattgc tctcgagcgc
     4741 cgaattgagc atgggaatgg cgacgtcatc ctgccgggcc cggtgagtca gggcgcgcag
     4801 atccttgggc aggcaggagc cgccgaaagc gaaccccggc ttgagataca agggcgagat
     4861 gttaagcttg gtatcctggg tgaagacatt catgaccaca cggctgtcga cctgaaaatt
     4921 cttgcacatc cggccgatct cgttggcaaa ggccaccttg acggcatgaa aggcgttgtc
     4981 cgcatatttt atcatcgagg cctcacccgg ggccagctgg aagagcggag cgggcaggct
     5041 ggagtagagc gccgagagca gatccgccga ctcctgatca aagaccccga tcacggtctt
     5101 ggggggatgg taaaaatcat ggaccgaact cccctcgcgc aggaattcgg gattgagggc
     5161 gaagccgaaa tcgctgcctg catgcatgcc gcttgccgcc tcgagcgccg gaatggccat
     5221 ctcttcggct acgcccggca ggacggtgct gcgcaggacg accgtgatgc gatggcccgc
     5281 gctgcgcagc agccggccaa tatcggtgca gacccggttg acgtaatcca gatggaggct
     5341 gccattgccg ttgctcggcg tgccgacgca gatgaaaacc agatcgcggt ccgaaagatc
     5401 gggcgcggtg gtggctctga gccggccggc ctgacgctgg gccaggatca gctcctccag
     5461 cccctcctcg acgatgggag agagcccctc attgatcaga cgcaccttat gggcgttgat
     5521 atcgacgccg atgacgtcat gccccatctc ggccagacag gctgccgaga cgcaccccac
     5581 atatcccaag ccgaaaatgc tgatcttcat agggaacgga cctccagaaa ccactccaat
     5641 tacacaccag gctgcagccg tgaaccgtaa aagctccggc gcagatgata gccatagatc
     5701 gccctgagca gccgctgtga agcagcgcag ccgattcgtc gactgcagcc cgccaggaca
     5761 gtcagcggcc ggaccgacgc cccgttgacc agcagggtct tgaggcgata gcggcggcgt
     5821 tcggccgggg tgagacccgc gtcgtagagc ccgaatcgcc gggccagctc cgtgcccatt
     5881 tccggctgca gggcgtgcca gcgcgccacc gcctctccat agagcgcgcc cttgcgcaga
     5941 tactcctcca ggcggagggg atgatgatgc acagcgaggg tatcgctgtg gtagaaaaaa
     6001 cgcatgcctc tcctggccag ccggtaggcc agctcgagat cctccgccgc cggcaggcgc
     6061 tcctcaaagc cgtgaatctc cagcagtgcg ctgcgaggca tcgagctgtt ggccgtgcgg
     6121 tagcggcgga agtcgatctc gcccgtgcgg gcatcgccgg ccgccagccg cagctcatgc
     6181 cagcgcgcct gcttcggtcc catcagctcg gcggacattt cgatccggcc cagatggcat
     6241 tcagcgcccc gggcgcggct gtgcagctca agatggcggc gcagcaggga aggcgaaggg
     6301 atggtgtccg cgtcgagaaa gaggatgatc tccccgcggc tggccgccag ggcgcggttg
     6361 cgcgctgccg ccgcgccctc tcccccgccc tgcagcgaga tgagcgtgaa gggggtcttg
     6421 ccggcctgtt cgcgcagatg gtccatggtg ccgtcgctgc tgccgtcgtc gacgacaatc
     6481 acctcaaagt gttcgggcgc cagctcctgc ttgcagaggc catcgagcac gcgccgcagc
     6541 acatcgagcc ggtcgcgcgc cgggatgact accgaaagat gcagcggtcg ggagttcatg
     6601 ccatctcctc cctgcactgc ggccaggccg cccgccagag ctgacggagg tcgctgcggt
     6661 cgatcgcctg cagcagccac agggccgtga gatagacact gccgccggcg gcggcgcgca
     6721 aaaagaaatt catcccctgc gaaaaaagca gggtcagggc gagcagggcg gccgaggcga
     6781 gcagggccag ggtgcggcgg tccgggcgca ggggatagag cagggtgtgc accgacccga
     6841 gctcgacgac agcgaaaacc gccgagcccg ccaccattgc cgccgccccg ccaattgccg
     6901 agaacctggg gatcagcagc agattgacga tgaccaccgt cgctgcggcc agaaaattgg
     6961 ccatgagatc acgcccctga cgatgatggc tgaccagggt gtaggccagg accggaatga
     7021 caccgtaggg caccaccatc cacgccagcc agcgcagaac cggtatcgcc ggagccatgg
     7081 ccgggccata gagaaaaaga atcaccggct ccgccagcag gctgagcacc agggccgcac
     7141 cgatgaagag catgagcaga tagtgcagcg cacggcgcag cagccggctg taaagcgcgc
     7201 gatcccggcg ggcgaggcgc gcggcaaccg gaaagagggc ctggctgaag gcgactgcga
     7261 agagaagtac atagctgatc agcttgtagg cggccgaata gtagcccgct gctgtctccc
     7321 cctcaagcca ggtgatcagc accaccgtga tcgaccagaa gagggcgttg aaaaaggcaa
     7381 tcgagagaaa aagcggcacc tgctgcagca gataggcggt cattccccgc tccagacgcc
     7441 agatgggccg ggccaccctg ctgccggcga gatgataaag cagcactgcg gcggccacct
     7501 tgctcagggc cagagccgcg acaacggcca tgatgcccct gccggcaaga agcaggccga
     7561 gaccgagaag tatgaggatg gcgttttccg ccagcgtgcc catgctgatg aactgcatct
     7621 tttcggcggc cgtgagcatg gcgcggctcc agtaggcgag acaggtaaag ggcagcagga
     7681 acgccaggag gatgacaatg cgcagggtcc cgggatcggg gcggatcagc cacgccgcac
     7741 cagccatcag cactcctccc gccgccgagc tcgccagccc gatgatcaac atgtggccga
     7801 aataggcggc cgccgtggcg ggattctgcg ccgcatcccg cgtcagcacc gaggagagac
     7861 cgaggctgga gaccagctcg aaaaagccca tcaaggcgag ggcgaaagag atcgccccga
     7921 aagtgccggc gccgagatag cgggcgagca ggatccagaa cagcgcggag ccgagccggg
     7981 cgacgacatc cgccagcgag cgccagaccg tatttttaat cagggtttgc agcatccgcc
     8041 gtctcttcaa cagtgattcc gccggatttc ccggcgacga tcagcaagcc attgccatct
     8101 cgattgacgc tgaaatagtc gatggccatg agcaaaagca caaaggggtg caccagcacc
     8161 agataaaagg ggaacaagac gagaaacagg gaactgaact gcaccgccgt gaccagatca
     8221 taggccagcc gcccgcaggg gccatagctg agctcacgcc gggtcacctc gaatccgcac
     8281 tgccccagcc gcgaggcaag cagttccggg gtataatagc ggacatggcc gtggggaagc
     8341 tcgccgcccg cagccctggt catcgccgcg attctgcgtt cgagggattt cagcacccgc
     8401 cgctcggaaa ggggcacata gatgagcagg gacccccgct cgcgcagcag ggcgtggaaa
     8461 cgctgcagca gggcgagatc atcggggatg tgctcgagca cgctgcagca aagaatgaga
     8521 tccaccgctc cgcccagatc atcccgggtg atgtcgccgg cgcggacctg cacccgctcc
     8581 agaccgagac gctgaaaaaa atccgccagg tccgcggcct gctccgcatc ctgctcgacc
     8641 gcctcgatgc gcagggcggg tgcacgccgg gcgagggcat aggtgtgttg gcccatgccg
     8701 caccccgcat cgagggcgct ggacatgtcc ccccgggcgg cgagcagacg gcgcatcgcc
     8761 ctggcgatgt accattcgcg cagggtggtg aggtgtataa gggcgtatcc cgcccggcgc
     8821 cgccagaggc tgcggccgat caggcgtgag atctgcttgg aattcatctc agcttttctt
     8881 cccggcaagt tcgacggcct gatcccgcca gctcctgtcc agatggatca gggcttcggt
     8941 gatggccgcg atggtccaga aaaggtacat cagggtcaga cttgatttat agccgtcggc
     9001 caggctggcg agcagaaagc agacgacact gacaaagagc ccgaccgcga tctcgcggaa
     9061 aaatttcacc cggcacttca tcgccgtcat tgtgcgcacc agcagggccg cgagcagcca
     9121 gagaaaggct gcaaagccaa taaggccgat ttcagcgagg atgagcagat aattattgtg
     9181 caccactgcg accttggtga aggggtcttc aatgcgccag tattcccgga tataactgtc
     9241 atagttattc acccccacac caagcaccgg atgatcgttg atgatctcca gcgcgacgcg
     9301 tgctgtggtc agacgcgatt tggccgaacc gtggtcgtcg ccggtcagcc ggttgtaaac
     9361 ggtctctccg taaaagaatg ccagaccggc gacgagcagc agcgccatcc cgatccgctt
     9421 cagggtgcgc agggtgacca gatttctcat cagtataaaa accatccccg ccatcatggc
     9481 gaccagaaaa gccccccaga cgctgcgcga ctgggtgagg atcagggcga ccaggccgcc
     9541 catggcggcg agcagggaga gcagaaacat cttgcgctcc cgggtggtaa gcatcaggat
     9601 cgaggcgatg ggcaggatga aaccgagata gcgggcgaag ccattgacgt ttcctaccgt
     9661 accgccggga cgcagaacca ttcgcgcttc ccgcttgtag gcgacaaaat tgctcaactc
     9721 ctcagtgccg accagcccca gggccgatcc gctgtagtac tgtgccacca tgacccccga
     9781 ctccatcacc agaccgaaga gcaaggccgt gaccaccagc cccacatccc ggttgtcgcg
     9841 gatgttattg gccagataga gaaaaaagat caacatcttg ccatacatgg cgatgtcgaa
     9901 gaggctcagg gtgatattgc gcgccgcaag tatggaaaaa ctgctcatga tgatcagcgc
     9961 cagggtcggc agggtgatcg ccggaaacca ctggatgcgc tcacggttgc gttcgtgcac
    10021 cgccgtcacc agccactgca ccagcaggat gaagagaagg atctctgtcg tcccgatggc
    10081 gatgccgctg tggccggaga tgctgcgctg gtagaaaaac ttgtagtcca ggccgagggg
    10141 gatcagaaag agcatccacc acatcacaaa ccggcggaaa tccgggacga tggcgagcac
    10201 gaagggcatg agcaggccga tgaaagcgta catcgaccag cccgcgcgca ggccccagat
    10261 gaaccaggcg gcgctggaga cacccaccgc gatggccgcc aggatgagca gattgatgat
    10321 ctgccgtaag atggatcgtt cttcattatt cacgctgtag gaattcatac ccgtactgca
    10381 cctttttgat tatgcccgct ccaggcccta gacgagacgg ttataaaaac cgtccgggat
    10441 ggtgatcttt tgccggttca gggcgacccc gagcaggttg gcctgggcga ccgacagctt
    10501 gcgctttgca ttctgggcca cctcccagcg ggtttgtccg gcgcgcacga ccaggatcac
    10561 cccatcggag agggctgaaa gggagagtgc gtcgacataa ttgagcacag cgggggcatc
    10621 gatgatgacg tagcggaact cctcccgcca gctcttgacc agttcgcgca gttcctcgga
    10681 gccgacgatg tcggccggct tgcccttggc cgcccctgcg gtgatgatgt gcaggtcgcc
    10741 ctggctgaga caccgggtca gcttgcgcca gtcccgcttc tcttcgatga tctccgccag
    10801 cccctcatcc tgttcgatcc cgaaaaagcg gtggagggcg ggattataga gattggcgtc
    10861 gaccaggagg acgccgccct ttttgatcgc ctgaatcagg gcctcggccg cctctttctg
    10921 gatatagtcg gtgaaccggg attgaaaaat ctcctcgccg gcccgctgct cctgcacggc
    10981 atcttcagac gtgctcgcgg attccgccgg agcgtcctgc tccggtgcat ccgccagggc
    11041 aagctccgaa ccggcgataa agtcgctccc ggccgcctca gcctgcaccc tttcggccag
    11101 ccccttctcc ataccgccgg agagcagaaa ggcgagcagg gtggcgatgg tcgaagagcc
    11161 ctcgcccgag atcgagctgg cgatggtgag aacgcgcatc tggctgcgct gtccggcgat
    11221 gcggatgtat tcgcgcagat cgtaaaaatc ggagaggatc tcctccggca ggctgcaggg
    11281 caggatgccg gtttggccgg actcttcccc ggccggccag ggcggagcgg ctatgccctc
    11341 tttggcgtca attttttcca gttcatcgaa aatagcactc atgggaacac ctcatcgttt
    11401 tcggaaaaaa ttaataattt actaaaaaat cgaaagttca cccaggtcga ggccgtcaaa
    11461 ccccgcggcg aattccagtt ccaccgccgg ctcgactcgg gggcgccgcc gggcaggcag
    11521 cggttcgctc tgttcatcag ctggggcagc ggcctcaccg gtagcagggg cggaacagat
    11581 ttccgcggcg cgctcaataa tctcggctgc ggcagctgcg ctgccggcgg gaggcaccca
    11641 gctctcccct tccggtgcgg cattctcgct cttttccgcc tggccggggt cgccagccgg
    11701 ccccgcctcg gccgtcatca ccttgtcttc ctgcaccaga tcgaggatca gggctgcgtc
    11761 gatctgcctg accttctgtg cggccgccgc catcagtgca ttgtcacaca cctgattgat
    11821 caggcggggg atgccgtcgc tgacctcgaa gatgacctgt agggctgcct cggtaaagat
    11881 ctcctgcttg cgcgcccctg ccaccttgag ccgatgccgg atataggccg aggtatcggc
    11941 ctcattgagc cggtgcaggg tcgccttcag gctgatgcgc tgcttgagct gcaccagggc
    12001 cggatcgttg agatagcggt cgagctgggg ctgcccgctg aggatgacct gcagcagctt
    12061 ctggcttgcg gtctccaggt tggagagctg gcggatctcc tcgagcacct cgggtttgag
    12121 gttctgggcc tcgtcaatga tcaggatcga aaagcgcccc tgctggtatt cgcggatcag
    12181 aaaggtgtag agctcgatga gcaggtcgcc gaggtcctcc ccctt
//
LOCUS       DAOPWU010000072        12203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5307_length_12203_cov_4.831824, whole genome shotgun sequence.
ACCESSION   DAOPWU010000072 DAOPWU010000000
VERSION     DAOPWU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12203
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5307_length_12203_cov_4.831824"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKM23_05575"
     CDS             <1..612
                     /locus_tag="PKM23_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HNY90979.1"
                     /translation="PKGPKILHPAYTRIDPNIGLATRCVPGKKENGAVFNHPVSWAIL
                     AECLLGHGERAFEIYKKAQPMNPVIDIDRYEVEPYVYAEYVTSPDHPTFGQASHSWLT
                     GSSAWMLRDGIDYILGVRPEYEGLRIDPCIPAAWRSYKVTRRFRGVTFHIRVDNPQGV
                     NKGVRRISLNGQPYEGTLIRIEEARLAGMLRQKSELDVVVEMG"
     gene            609..1316
                     /locus_tag="PKM23_05580"
     CDS             609..1316
                     /locus_tag="PKM23_05580"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005320372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HNY90980.1"
                     /translation="MIDHKIDFIAAVRGRLIVSVQADAGDPLDDPHILTAMAQAVVRG
                     GAAAIRACQPANIAAIRAAVGVPLIGLSKKEYRDSSVYITPTLADALAVAATGCAVIA
                     LDATLRPRPGGETLGTIVAGLRQATSALLMADVATVQEGAAALELGFEIISSTLSGYT
                     AESSGSDPCQPDYELVAALVREAGGRAAVIAEGRIWHPEEAQRLLALGAHAVVVGTAI
                     TRPGAITRRFIEMMQPR"
     gene            complement(1413..5759)
                     /locus_tag="PKM23_05585"
     CDS             complement(1413..5759)
                     /locus_tag="PKM23_05585"
                     /inference="COORDINATES: protein motif:HMM:NF026756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4623 domain-containing protein"
                     /protein_id="HNY90981.1"
                     /translation="MKKVMVLFSMLMLLASAAFALVDSTATPIWKVSADDAAQFFFKN
                     NDLTRSIALNKVTNHLLVATRTGGHRIVALNAAKGDSVGQLDMTGVSGGTYHLNKVGV
                     ADDGVIYACNLNIGSGLKIYRWADEAALPTVAADTTVTGATRFGDAFHVVGQGLATKI
                     YISGNNAAAKIHVLGTTDGLKFQVEKVIAKNGHCTDIYPLDENTIWVNRPGIAATRLD
                     GAGEITGTVPTTVIGTSASALDVFGYGGYFYLASADGNVKPATGRLVQLGDNLADSRV
                     NVVFKGMGSNNNANGVGEVVVQPDSDRVWVLETNNAIALYPFGGYASFPLAWRSKADT
                     AAWHGTNSMVRTLAYSAKTKHLYVASRVGGTFIKVFDAATGHYLMDLNTAGIAGGTYH
                     INMVTATPDGQIFAGNLALANGTFKLYHYASEGAAPVLAWEGVVPGRAGDAMACTGTG
                     KNVTVYVSGIDNDKIYTFVSTEGAAFVQGADIPLPEANAARFGIAPVGNGDYLFVNGP
                     AKPTRYIKKDGTVLHQFDTAEVVGAAVNFAEIPALDGTSRKFLFLANGWTPGVRVVEL
                     FGEEGDNLCAYWEKWPAMTPLYGSNPNPNANAQAVYDIYSNHLIELATNNGLSAYSFA
                     NVMLNAGMLEADPVFSTNNLHFGNVLLGTAAQQSFSITNQGTAPFEILSAGFDGDRFS
                     SNLTGPATVDVGATLVVTISLATDIAGDFDDTFTLLTNRGVYEVELFGVVERFWPFTL
                     KELDFGSVWARSTNVLDFHLINSGAAPITVDSTAWDATLMSFSPDSSITVAAGDTANL
                     TVTLKPSTTGAIASNLLVYTSLGLYDFPVKAMAEELWPLNWRQTADTTLWHGTNNMVR
                     ALVYNKATNHLLTVSRVGGSFIKALDPKTGKVVKEINTTGINGGTYHINVLAASDDGQ
                     IFVGNLALAGTNYKLYYLANEDAAPVMIFDGLLEGRVGDAIGVSGTGVNVIAYASGSD
                     NTKIFTFSTTDGVTFSRGADIPLPEKSAARYAISPVNHDYLFVEGVGTRARYLKKDGT
                     VLFEFDRTLLGGTTCTYFEVVTDEEITRRFLAACDGFSPGTRVVELLGAPGDNLCQFF
                     EILPANTPKWATVANANATSQIAFDRVNNQLVELVTNNGISAYSFAEVVPNPKSYQLI
                     MPIAAAKVDGDGDFKPDLAGQIVTIQGVVTTINYNSGNSSSYYLQDEQGWGINFYSSK
                     INYNLEMGKRVQITGKIEFYNGLTEITATDSAAVQVLGDGMVPAPLEITSGAEMSERT
                     EGSLVRLTGYYLEKPASWPAAGKNATLTFVRGADTVLVFIDKETDIDGTPAPQGYYAL
                     TGVIDQYTTSVPPNNKYEIRPRSLADLSPVTGMARHDQELPTTWALQQNYPNPFNPVT
                     RIAFAAPQSGEVRIKVYDLLGKEVASIFEGRLEAGFHTFDFNGSSLTSGVYFYRVDAG
                     GFTELKKMTLVK"
     gene            complement(5902..7752)
                     /locus_tag="PKM23_05590"
     CDS             complement(5902..7752)
                     /locus_tag="PKM23_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNY90982.1"
                     /translation="MRIRNIWLLLLLSAAGMAGAQEELRGVWIAWGGANPPTKNAIAA
                     MMEDIAAHNLNTVYVDVWRYGYPYFRSRTFYDLTGQWTDPRLENGRDVLADMIAEGHR
                     AGLHVEAWFEYGFCACHDQNDDLYRARPEWFAQHRDGSVLFNGSLRWKWLSHLHPQAQ
                     QFLIDLCQEVAANYDVDGIELDRIRYPELDCGYDPATVAAYQADHGGAAPPAAPGDRS
                     WMAWRAQKLTDFVAAFHDSIKAVHPDLLISNAPISYSYGYDNFCQDWRPWINEGHLDV
                     VSTQLYWPTNAVYAAELDRQLSYITARERFFPGLCSIANEIIVPATEIAAMIRTTRAR
                     NLQGHVIWYYNTLADDLPYLRDEVYQQPAAVPGRPREWRLPARVINEEEAGASRSEGW
                     IPYTTLRGFKEGCLYCRAGEERWIEYTADIAETGWYELYTFNIYQFSASQRAPYEIRH
                     AAGSDTVYVNQALSGQARWFKLGDYFFRQGVGQRVVRLSNAGIEANAVLFADAIMLIR
                     SRRPLGMPALVARQPEQVLPAASSILLENYPNPFNAATRIRFTLVQSAMVELRIHDMR
                     GREVAVLARGRHDAGVHEVTWQAQAAGSGLYYGLLITESGKSIRKLLLLK"
     gene            complement(7769..9718)
                     /locus_tag="PKM23_05595"
     CDS             complement(7769..9718)
                     /locus_tag="PKM23_05595"
                     /inference="COORDINATES: protein motif:HMM:NF026756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4623 domain-containing protein"
                     /protein_id="HNY90983.1"
                     /translation="MKRLLLISLLIFAAAGLLQAEYPLLWKTNKFSDPANDICRGLVY
                     NPDTDHVLVVTRKGGVHIYILDAASGDSLGEMNSAGIGGGTYAINLIARADDGTLYVC
                     NLSAPVYSPGSKFKVYRYAGETAAPELVFEDALENGRFGDSFAAAGHGAGKYLYSSGQ
                     DNPRIAVLKDTGGSSLIFDRYVNLPVPGGARHGISPVAPGGKLWINSAGPLFPPTLIT
                     ADGTIIATCPDTLASPGGTSGVTHLQLGLFKYIVVANGYSANFRTVKYFEDELGTVTF
                     DYSGANSDSLASIYAPGTKGSNANATGLSSYDSRRNALVTLVGMNSIASISFDKMLKT
                     STPRDSALTVSIDGLNDFFPTDCVGKGALHTLCFTWSEGKLFLGLTGAALIEPAGKNR
                     LYWAFDLDPEGLNGSTVPPTAAGGVKALPFRADVVYEVESWNAADFMVGSIYKWKGSA
                     WSATAFDGNLAGQGALAYAVADFAELSAIKNEPGIGASANRLAMMAYVAEKGSSGQVL
                     AAFPVDNPAGSAPTFNRYYYVNELGSGLFPVDPGAVEVRSSGASGVDGLAEAQPAHFR
                     LLQNYPNPFNAATTIAFELGRKGRVRLELYDITGRKAATLVDGLREAGRYAILYDGSG
                     LSSGLYHLRLVTEEGSLMRKLVLLK"
     gene            complement(9752..10384)
                     /locus_tag="PKM23_05600"
     CDS             complement(9752..10384)
                     /locus_tag="PKM23_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNY90984.1"
                     /translation="MKRYLFLICFAATAVPAAAVEQGRWGAAVQGIYSAPAGALTQWF
                     KPAANYAITAGQQYNRCWFLEGLLEYSSFSRENLTGYPEGRLELSLEHTALLFSGRYR
                     LAPVSMLHPYLHFGAGVLHWKGVRGEVEADPAIDLPHIDEKVLQEYNWSFRLGAGMEF
                     MISSSLSLDLFAHYRLVIGDLYPTMQPHIELEGVSGFQSLNAGLGLRYYF"
     gene            complement(10398..11216)
                     /locus_tag="PKM23_05605"
     CDS             complement(10398..11216)
                     /locus_tag="PKM23_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90985.1"
                     /translation="MRSILNKAFFLALIILPAAAAHAQERPIQTRLFGSVQQLVDKGR
                     IEAGSGYGAGAELSTRLWPGVRLRAALHYEVAGLEQAGVLDEWQWDYWEKTYIPFLPG
                     ANLTRINQELVYTSTDSIYSAVFEPTQKMNELRFSLGLTWEIPLGAGFTPYLGLSGGL
                     DLYKRQLEMEEHWIKRFDLDTLRAGLDYEYPLDVLHFAPPKQGKRLFIMPTLGLRYTL
                     STSVDLDGTAHYVYYPGREAIAGLENLFNISSDSAQWFPFKSKLLLTLGLTFKY"
     gene            complement(11220..>12203)
                     /locus_tag="PKM23_05610"
     CDS             complement(11220..>12203)
                     /locus_tag="PKM23_05610"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY90986.1"
                     /translation="RRPLRMKIIGQRTALAGMLLLLGWAAAGMAQEFNKTGTSGFVFL
                     EIPASARFAALGETGITMPDAGAEGLFVNPALTALGSKRYGLSVSHAGWYVETSHQAF
                     ALTAQMARIGVLGLHATWFDFGRMEKTINPSAAQAGSYISLGEYTAGAYALGLTFARQ
                     LTDRFAFGASLNYVREEIDLWHADNVVTDIGFVFMTGYHSLRIGTFLQNFGLEAKYAR
                     EKFKMPQQLKMGLSADLAGGFESPNRLTLLLEAVQPNDGDGRLHTGLEALLAGALYLR
                     GGYKYGYDHENLTGGIGLRLETALGRLRCDFSYMEHETLDGTARYTLVMEF"
BASE COUNT         2370 a         3647 c         3872 g         2314 t
ORIGIN      
        1 cccaagggcc ccaagatcct ccaccccgcc tacacccgca tcgaccccaa catcgggctc
       61 gccacccgct gcgtgccggg caaaaaggag aatggcgcgg tctttaacca cccggtgagc
      121 tgggcgatcc tcgctgaatg cctgctcggc cacggcgagc gcgccttcga gatctacaaa
      181 aaggcccaac cgatgaatcc ggtcatcgac atcgaccgct atgaggtcga gccctatgtc
      241 tatgccgaat atgtcaccag tccggaccac cccaccttcg gccaggcgag tcactcctgg
      301 ctcaccggct cttcggcctg gatgctgcgc gacggcatcg actatatcct tggggtgcgg
      361 cccgaatacg agggactgcg catcgacccc tgcattccgg cggcctggcg cagctacaag
      421 gtcacccgcc gctttcgcgg cgtcaccttt cacattcgag tagacaaccc gcagggggtg
      481 aacaaggggg tgcggcggat cagcctcaac ggccagccgt acgagggtac cctgatccgc
      541 atcgaagaag cgcggctcgc cggaatgctg cggcaaaaaa gtgaactgga tgttgtcgtg
      601 gagatgggat gatcgatcac aaaatagatt ttatcgcagc tgtgcgcggc cggctgatcg
      661 tctcggtaca ggccgacgcg ggtgaccccc tcgatgaccc gcacatcctg acggcgatgg
      721 cacaggcggt ggtccgcggg ggggcggccg cgatccgcgc ctgccaaccc gccaacatcg
      781 ccgccatccg ggcggcggtg ggggttcccc tcattggcct gtccaagaag gaatacaggg
      841 attcttccgt ctacatcacc ccgaccctgg ccgatgccct cgcggtcgcc gcgaccggct
      901 gtgctgtgat cgctctcgac gccaccctgc ggccgcggcc gggcggcgag accctcggca
      961 ccatcgtcgc cggcctgcgc caggccacct ccgccttgct catggccgac gtcgccactg
     1021 tacaggaagg ggccgccgcc ctcgagctcg gctttgagat catctcctcc accctctccg
     1081 gctacaccgc agagagctcc ggcagcgatc cctgccagcc cgactatgag ctcgtcgccg
     1141 ctctggtgcg cgaggccggc ggccgggcgg cggtcatcgc cgagggccgc atctggcatc
     1201 ccgaagaggc gcagcggctg ctcgccctgg gcgcccatgc cgtggtggtc ggcaccgcca
     1261 tcacccgccc cggcgccatc acccgccgct tcatcgagat gatgcaacca cgctgatccg
     1321 gccccaatcc ccccacataa aaaaatgctg tggcgccgcc ctgagagggc gggccacagc
     1381 atttctgctg cagaggcctg attacctggc cgttatttca cgagagtcat ctttttcagc
     1441 tcggtgaagc cgcccgcgtc gacgcggtag aaatagacac cgctggtgag gctgctgcca
     1501 ttaaagtcga aggtatggaa cccggcttcg agccgtccct caaagatgct cgccacttcc
     1561 ttgcccagca gatcatagac cttgatccgt acctcaccgc tctgcggagc cgcaaaggcg
     1621 atgcgggtga caggattgaa ggggttcgga tagttctgct gcagggccca ggtcgtgggc
     1681 agctcctgat catgccgcgc catgccggtg accggggaga ggtcggccag cgagcgcgga
     1741 cggatctcgt acttgttgtt cggcggtacg ctggtggtgt actgatcgat cacgccggtg
     1801 agagcatagt agccctgggg ggcaggcgtg ccgtcgatgt cggtctcctt gtcgatgaat
     1861 accagcacgg tatccgcgcc gcggacgaag gtgagggtgg cattcttgcc ggccgccggc
     1921 cacgacgccg gtttttccag atagtagccg gtgaggcgca ccagagaacc ctcggtgcgc
     1981 tcgctcatct ccgctccgct ggtgatctcc agcggcgccg gaaccatgcc atccccgagc
     2041 acctgcaccg ccgcagaatc ggtcgccgtg atttcggtca gaccattgta gaactcgatc
     2101 ttgccggtga tctgcacccg tttgcccatc tccagattgt aattgatctt gctggagtag
     2161 aaattgatgc cccagccctg ctcatcctgg agatagtagc tcgacgagtt gccggaattg
     2221 tagttgatgg tcgtcaccac accctggatg gtcacgatct ggccggcgag gtcgggcttg
     2281 aaatcaccat ccccatcgac cttggctgcg gcgatgggca tgatcagctg gtaactcttc
     2341 ggattcggca ccacctcggc gaagctgtag gcgctgatgc cgttgttggt caccagttcc
     2401 accagctgat tgttcacccg gtcgaaggcg atctggctgg tggcgttggc attggcaacg
     2461 gtcgcccatt tcggcgtatt ggccgggaga atttcaaaga actggcacag gttgtcaccc
     2521 ggtgcgccga gcagctcgac cacgcgggtc ccgggcgaga aaccgtcaca ggcggcgagg
     2581 aagcggcggg tgatctcttc atccgtgacc acttcaaaat aggtgcaggt cgtgccgccc
     2641 agcagggtgc ggtcgaattc gaagaggacc gtgccatcct ttttcaggta gcgggccctg
     2701 gtgcccacgc cttccacgaa aagatagtca tggttcaccg ggctgatggc atagcgggct
     2761 gcgctctttt ccggcagggg gatatcagct ccccggctga aggtgacgcc gtcagtggta
     2821 ctgaaggtaa aaattttggt attatcggag ccggaggcat aggcgatgac attgacgccg
     2881 gtgccggaaa cgccgatcgc atccccgaca cggccctcga gcagaccgtc aaaaatcatc
     2941 accggtgccg catcctcatt ggcgagatag tagagcttgt agttggtgcc ggcgagggcg
     3001 aggttgccga cgaagatctg tccatcgtcg ctcgccgcca gcacattgat atgataggtg
     3061 ccgccgttga tgccggtggt gttgatctcc ttgacgacct tgcccgtctt gggatcgagg
     3121 gctttgatga agctgccgcc gacgcgactg accgtcaaga ggtggttggt ggccttgttg
     3181 tagaccaagg cccgcaccat gttgttggtg ccgtgccaga gggtggtatc ggccgtctgg
     3241 cgccagttga ggggccaaag ctcctcagcc atcgccttga ccggaaagtc atagagacca
     3301 agagaggtgt aaacgaggag gttgctggcg atggcaccgg ttgtggaggg cttcagggtg
     3361 accgtcaggt tggcggtatc gccggcagcc actgtgatcg atgaatccgg tgagaagctc
     3421 atcagggttg catcccaggc ggtcgaatcg accgtgatcg gcgctgcacc tgaattgatg
     3481 agatggaaat cgagcacgtt ggtggaacgg gcccagacgc tgccaaagtc aagttccttc
     3541 agggtgaaag gccagaacct ttcaaccacg ccaaagagct ccacctcata gactcccctg
     3601 ttggtcagca gggtgaaggt atcatcgaaa tcaccagcga tgtcggtggc cagggagatg
     3661 gtgacgacaa gagttgcgcc gacatccact gtggccggac cggtgagatt gctgctgaag
     3721 cgatcgccgt caaatccggc ggagagaatc tcgaaggggg ctgtgccctg gttggttatg
     3781 gaaaaactct gctgcgcggc ggtgccgagc agcacgttgc cgaaatggag attattggtc
     3841 gagaaaaccg gatcggcctc gagcatgccg gcattcagca tgacattggc aaaggagtag
     3901 gcgctgagac cgttattggt ggccagttcg atcagatggt tgctgtaaat atcatagacc
     3961 gcctgggcgt tggcattggg attgggattg ctgccataga ggggcgtcat cgccggccat
     4021 ttctcccaat aggcgcagag attgtcgccc tcctccccga agagttcgac gacgcgcacg
     4081 ccgggggtcc agccattggc cagaaagagg aacttgcgcg aggtgccatc gagggcgggt
     4141 atctcggcaa agttcaccgc cgcgccgacg acctctgcag tgtcgaactg gtggagcacc
     4201 gtgccgtctt ttttgatata gcgcgtgggt ttggccggcc cgttgacgaa gagataatcc
     4261 ccgttgccga ccggagcgat gccgaaacgt gcggcgttgg cctcgggcag ggggatatcc
     4321 gctccctgaa caaaggcagc gccttcagtg gagacgaatg tatagatttt gtcgttgtcg
     4381 atgccggaaa catagacggt gacattcttg cccgttcccg tgcaggccat ggcatcccct
     4441 gcgcggccgg ggacgacacc ctcccaggcc aggaccgggg cggccccttc gctggcatag
     4501 tgatagagtt tgaaggtgcc gttcgccagg gcgaggttgc cggcgaagat ctggccgtcg
     4561 ggcgtggcag tcaccatgtt gatgtgatag gtgccgcccg cgatgccggc cgtattgaga
     4621 tccatgagat aatgacccgt agcggcatcg aagaccttga taaaggtgcc gccgacccgg
     4681 gaggcaacat ataggtgttt ggtcttcgcg ctgtaggcaa gagtgcgcac catgctgttg
     4741 gtgccgtgcc aggcggcggt atcggctttg gaacgccagg ccagcggaaa agaggcataa
     4801 ccgccgaagg gatagagagc gatggcattg ttggtctcca gcacccagac gcggtcgctg
     4861 tcgggctgca cgaccacctc gccgacgccg ttggcgttgt tgttcgagcc catgcccttg
     4921 aaaaccacat tgaccctgga atcagcgagg ttgtcgccga gctgaaccag gcggccggtg
     4981 gccggcttga cattgccgtc ggcgctggcc agataaaaat agccgccata accgaagaca
     5041 tccaatgccg aggcgctggt gccaatgacg gtggtgggca ccgtgccggt gatctcgccg
     5101 gcgccatcga gacgggtggc cgcgatgccc gggcggttga cccagatggt gttttcatcc
     5161 agggggtaga tgtccgtgca gtggccgttc ttggcgatca ctttttcgac ctggaattta
     5221 agcccatcgg tcgtgccgag aacatggatc ttggctgctg cattgttgcc ggagatatag
     5281 atcttggtcg ccaacccctg gccgacgaca tggaaggcat cgccaaaacg ggtggcgccg
     5341 gtgacggtgg tatcggcggc cacagtcggg agcgccgctt catccgccca gcggtagatc
     5401 tttaagccgc tgccgatatt gaggttgcag gcgtagatga cgccgtcatc ggcaaccccc
     5461 accttgttga gatgataggt tccacccgag acgccggtca tgtccagctg gccgacgctg
     5521 tcccctttgg cggcattcag ggcgacgatg cgatgcccgc ccgtacgcgt agccaccagc
     5581 agatggttgg tcaccttgtt cagggcgatg ctccgcgtca aatcattgtt cttgaaaaag
     5641 aactgtgcgg catcatcggc gctgactttc cagatcgggg ttgctgtgct gtcgaccagc
     5701 gcaaaggccg cactggccaa gagcatcagc atggaaaaca gtaccattac ctttttcatg
     5761 catctcctcc acgaattggg ataagggatt tcctgcggca gcggcatttc gagaggcggc
     5821 agagggatat caccaggatc tcttcggtct tgtgcgatcc cgccgctgga ggcaggcctg
     5881 gtttgtgaat gtactaaggt tttatttaag gagcaagagc ttccgtatgc ttttgcccga
     5941 ttccgtgatc agcaggccgt aatagaggcc gctgcccgca gcctgcgctt gccaggtcac
     6001 ctcgtggacg cccgcgtcat ggcggccgcg ggccagaaca gcgacctcgc gcccgcgcat
     6061 atcatgaatg cgcagctcca ccatcgcgga ctgcaccagg gtgaagcgaa tgcgcgttgc
     6121 ggcgttgaag ggattgggat agttctccag cagtattgag ctcgcggcgg ggagcacctg
     6181 ctcaggctgc cgcgcgacca aggccggcat tccgaggggg cgccggctgc ggatcagcat
     6241 tatggcatcg gcgaaaagca cggcattggc ctcgatgccg gcgttgctca ggcgcaccac
     6301 gcgctggcct actccctggc gaaagaaata atcacccagt ttgaaccagc gcgcttgccc
     6361 tgacagggcc tgattcacat acacagtatc gctccccgcg gcatggcgga tctcgtaggg
     6421 cgcccgctgg ctcgccgaaa actggtagat gttgaaggta tacagttcat accagccggt
     6481 ctcggcgata tcggccgtat attcgatcca gcgctcttct ccggccctgc agtagaggca
     6541 tccctccttg aaaccgcgca gtgtcgtata ggggatccag ccctccgagc ggctcgcgcc
     6601 ggcttcctcc tcattgatca cccgggcggg gagacgccac tcgcgcggcc gtccggggac
     6661 ggcggcgggc tgctgataga cctcatcccg aagatagggc agatcgtcgg ccagggtgtt
     6721 atagtaccag atcacatggc cctgcagatt ccgcgcccgc gtcgtccgga tcatggcggc
     6781 gatctcggtg gcggggacga tgatttcgtt ggcgatggaa cagaggccgg gaaagaacct
     6841 ctcgcgcgcg gtgatatagc tcagctgccg gtcgagttcc gcggcataga ccgcattggt
     6901 cggccagtag agctgggtgc tgaccacatc cagatggccc tcattgatcc agggccgcca
     6961 gtcctgacaa aagttgtcat aaccgtagct gtaggagatc ggggcgttgc tgatgaggag
     7021 atcgggatga accgccttga tcgagtcgtg gaaggcggcg acgaaatcgg tcaatttctg
     7081 cgcccgccac gccatccagc tgcggtcgcc cggcgccgcc ggcggcgcgg cgccgccatg
     7141 gtccgcctga taggctgcga cggtggccgg atcgtaaccg cagtccagtt ccggatagcg
     7201 gatgcgatcc agctcgattc catcgacatc atagttcgcc gccacctcct ggcacaggtc
     7261 gatgaggaac tgctgggctt gaggatgaag atggctgagc cacttccagc gcaggctgcc
     7321 gttgaagagg acggagccat cgcggtgttg cgcaaaccac tccggccgtg cccgataaag
     7381 gtcgtcgttc tgatcgtgac aggcgcaaaa tccgtattcg aaccaggcct ccacatgcag
     7441 ccctgcccgg tggccctcgg cgatcatgtc ggccagcaca tccctgccgt tctcaagtcg
     7501 cggatcggtc cactggccag tcagatcata aaaggttcgg ctgcggaaat agggatagcc
     7561 atagcgccag acatcaacat agaccgtatt gagattatgg gcggcgatgt cctccatcat
     7621 tgccgctatg gcattcttgg tcgggggatt ggcgcccccc caggcgatcc acaccccgcg
     7681 cagttcctcc tgagcgccgg ccatgccggc ggccgacagg agcagcagga gccaaatgtt
     7741 ccgtatccgc aaaagtggca ttctccttct acttgagcag gaccagcttg cgcatgaggc
     7801 tgccctcctc cgtcaccagg cggaggtgat agaggccgct gctcagaccg ctgccatcat
     7861 aaaggatggc ataccgcccg gcctcgcgca gcccgtccac cagggttgcg gccttgcgcc
     7921 cggtgatgtc atatagctcg agccgcaccc ggcccttgcg ccccagctca aaggcgatgg
     7981 tagtggccgc gttaaaggga ttgggataat tctgcagcag tctaaagtgc gcgggctgag
     8041 cttcggctaa accgtcgacc ccgctcgcgc cggaggagcg gacctcgacc gcacccgggt
     8101 cgacggggaa aaggccgctg cccagttcgt tgacgtaata ataacggttg aatgtgggcg
     8161 cgctgccggc gggattatcg acagggaagg ccgccaaaac ctggccgctg ctccccttct
     8221 ccgccacata ggccatcatc gccagccggt tggccgaagc gccgatgccc ggttcgttct
     8281 tgatcgccga gagctcggcg aaatcggcca ccgcataggc cagggcgccc tgtcccgcca
     8341 gattgccgtc gaatgccgtt gcactccagg ccgacccctt ccatttgtaa atgcttccga
     8401 ccataaaatc ggctgcattc caggattcca cctcatagac gacgtccgct ctgaatggca
     8461 gcgccttgac gccgccggca gccgtgggcg gcaccgtcga gccgttgagg ccctcgggat
     8521 cgagatcgaa agcccagtag agccggtttt ttccggccgg ttcgatcagg gcagctccgg
     8581 tgagccccag aaagagcttg ccctcggacc aggtgaaaca gagagtatgg agagccccct
     8641 tgccgacgca gtctgtagga aaaaagtcgt tcagtccgtc aatgctgacg gtcagggcgc
     8701 tgtcacgggg cgtactggtc ttgagcatct tgtcaaaact gatcgaggcg atgctgttca
     8761 tgcccaccag ggtcaccaga gcgttgcgtc tgctgtcgta gctgctcagg ccggtggcgt
     8821 tggcgttgct ccctttggtg cccggggcgt agatcgatgc cagcgagtcg gagttggctc
     8881 ctgaataatc aaaggtgacc gtgcccagct catcctcgaa atattttacc gtgcggaaat
     8941 tggccgagta gccgttggcg acgacgatat acttgaagag gccgagctgc aggtgggtga
     9001 cgcccgaggt gccgccgggc gaggccaggg tgtcaggaca ggtggcgatg atggtgccat
     9061 ccgcagtgat cagggtcggg ggaaagagcg gcccggcgga attgatccac agcttgccgc
     9121 ccggagcgac cggcgagatg ccgtggcgcg caccgcccgg caccggcagg ttgacgtagc
     9181 ggtcaaaaat caggcttgat ccccccgtgt ccttgagcac cgcaatgcgg gggttgtcct
     9241 gaccggagct gtagagatat ttgccggcgc cgtgtccggc cgccgcaaac gaatcgccaa
     9301 aacggccgtt ctcgagggca tcctcaaaga ccagctccgg ggcggcggtt tcgccagcat
     9361 agcggtagac cttgaacttt gaaccggggc tgtagaccgg cgcggagaga ttgcagacgt
     9421 aaagcgtgcc atcgtcggcg cgggcgatga ggttgatggc gtaggtgccg ccgccgatgc
     9481 cggcgctgtt catctcgccc agggaatccc cgctggcggc atcgaggatg taaatgtgca
     9541 ccccgccctt gcgggtgacc accagtacat gatcggtatc gggattgtag accaggccgc
     9601 ggcagatgtc gttggccggg tcgctgaact tgttggtctt ccagagcagg ggatactcgg
     9661 cctggagcag acctgcagca gcaaaaatga gcagggaaat cagtaacaac cgtttcatgc
     9721 caacctccga tgatcaggat cggttaaatt gctaaaaata atagcgcaag cccaggcccg
     9781 cgttcaggct ctgaaagccg gagaccccct ccagctcgat gtggggctgc atggtcggat
     9841 agagatcgcc gatcaccagc cggtaatggg caaaaagatc caggctgagc gaggaagaaa
     9901 tcataaactc catgcccgct cccagacgaa aggaccagtt gtactcctgc agcacctttt
     9961 cgtcgatatg cggcaggtcg atggccggat cggcctccac ctcaccgcga acccctttcc
    10021 agtgcagcac gccggcgcca aaatggagat aggggtgcag catgctcacc ggtgcaagtc
    10081 tgtagcggcc ggaaaagagc agcgccgtat gctcaaggga gagttccagc ctcccctccg
    10141 gatatcccgt caggttttcg cggctgaagg agctgtattc gagcagcccc tcgagaaacc
    10201 agcagcggtt gtactgctgg ccggcggtga tggcataatt ggcggcgggt ttgaaccact
    10261 gcgtcagcgc tcccgccggc gccgaataga tcccctgcac cgcggcgccc cacctcccct
    10321 gctccaccgc cgccgccggc acggcggtcg ctgcaaaaca aatcaggaaa agatagcgtt
    10381 tcatgtctgg tcgcgtctca atatttgaag gttaggccca gggtcagcag cagcttcgac
    10441 ttgaagggaa accactgcgc gctgtccgag gagatgttga acagattttc tagtcccgcg
    10501 atggcctccc ggccgggata ataaacgtaa tgcgccgtgc catccagatc gacggaggtg
    10561 gagagcgtgt agcgaaggcc gagcgtgggc atgataaaga ggcgtttgcc ctgcttgggc
    10621 ggggcaaaat ggagcacatc gagggggtac tcgtaatcga ggcccgcccg cagggtgtcg
    10681 agatcgaaac gtttgatcca gtgctcctcc atctccagct ggcgtttgta gaggtccagc
    10741 ccgccgctga ggcccagata gggcgtgaac ccggctccga ggggtatctc ccaggtgagc
    10801 cccagggaga agcgcagttc attcatcttc tgggtgggct caaagaccgc cgaatagatg
    10861 ctgtcagtgc tggtgtagac gagttcctga ttgatgcggg tcagattggc tccgggcagg
    10921 aaggggatgt aggtcttttc ccagtaatcc cactgccatt cgtcgagcac gccggcctgc
    10981 tccagtccgg ccacctcata gtgcagcgcg gctcgcaggc gcacccctgg ccacagccgg
    11041 gtgctcagct cagcgcccgc gccgtatccg gaacccgctt cgatccgtcc cttgtcgaca
    11101 agctgctgca cggatccgaa aagccgggtc tggatgggcc gctcctgagc gtgcgcagcg
    11161 gcggcgggca gaatgattag cgccagaaag aaggccttgt tcaggattga gcgcatggtt
    11221 caaaactcca tgacgagggt gtaacgtgcc gtgccatcca gggtttcatg ctccatatag
    11281 gaaaaatcac agcgcaaccg gcccagcgcc gtttcgaggc gcaagccgat gccgccggtg
    11341 aggttttcat gatcatagcc gtacttgtag cccccgcgca gatagagggc gccggccagc
    11401 agagcctcca ggccggtgtg cagcctgcca tcgccgtcgt tgggctgcac cgcctccagc
    11461 agcagggtca gacggttcgg actctcaaaa ccgcccgcca ggtcggcgga gaggcccatt
    11521 ttgagctgct gcggcatctt gaatttttcc cgggcatatt tagcctcgag gccgaaattc
    11581 tgcaaaaagg tgccgatgcg caggctgtgg tagcccgtca taaaaacaaa cccgatgtcg
    11641 gtcaccacgt tgtcggcatg ccagaggtcg atctcctcgc gcacatagtt cagggaagcg
    11701 ccgaaagcga agcggtcggt gagctggcgc gcgaaggtga gaccgagggc gtaggctccg
    11761 gccgtatatt caccgaggct gatgtaggag cccgcctgcg cggcggaggg attgatggtc
    11821 ttttccatgc ggccaaaatc gaaccaggtg gcgtgcagcc cgaggacccc gatgcgggcc
    11881 atctgcgccg taagagcgaa ggcctgatgg ctggtttcga cgtaccagcc ggcatgtgag
    11941 accgacagtc catagcgttt gctgcccagg gcggtcaggg cgggattgac aaagaggccc
    12001 tccgcgccgg catcgggcat ggtgatgccg gtctcgccga gggcggcaaa tcgcgccgag
    12061 gccggaatct cgagaaagac gaagcccgag gtgccggtct tgttgaactc ctgcgccatg
    12121 cccgccgctg cccagccgag cagcaagagc atcccggcta gagctgttct ttggccgatt
    12181 attttcattc tcaaaggcct gcg
//
LOCUS       DAOPWU010000073        12100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5381_length_12100_cov_4.766874, whole genome shotgun sequence.
ACCESSION   DAOPWU010000073 DAOPWU010000000
VERSION     DAOPWU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12100
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5381_length_12100_cov_4.766874"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PKM23_05615"
     CDS             complement(<1..459)
                     /locus_tag="PKM23_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNY90987.1"
                     /translation="MVAAGVFFSRIAGLIRDRVFAHFFGSSDAADAFKAAFRIPNFLQ
                     NLFGEGTLSASFIPVYARLLAEEREEDSGRVAGAVAAILSLVVALSVLAGVLLTPWLI
                     ALIAPGFSGAKRELTIQLVRILFPGAGLLVLSSWCLGVLNSHRKFFLSYAA"
     gene            complement(567..1841)
                     /gene="manA"
                     /locus_tag="PKM23_05620"
     CDS             complement(567..1841)
                     /gene="manA"
                     /locus_tag="PKM23_05620"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HNY90988.1"
                     /translation="MTSRRPAQSLYPLHGRVMHYPWGGYACIPGLLGVRNDEKRPFAE
                     LWLGSHPQAPSEVEVEGGRIDLVELLRREGERILGSAVLARFGAGLPFLFKVLDAREM
                     LSIQVHPSRAQSEAGCAAEEAAGMPLTDPRRNYKDRNHKPEVHVALSEFWMLHGFRPL
                     EEIADVLAGIPDFTGLSPWFPLHLLEVDEDPAGRAELLRRLYAFIMTMPQDEADRILG
                     GLLAHLEPLYDAGSLHKSSPHYWAVKAARSFPLPEGHIDRGIFSIYLMNLVELQPGQG
                     TFQGAGVPHAYLEGVNVELMANSDNVLRGGLTPKHVDVAELLKTIRFDDGRPEILTGV
                     VAEGGERLYPAPVDDFLLSRIELEPRGVHASGSGHGPDILIALEGEASIRAEGEELAL
                     RRGDALLAAANSPWRLHSSSGALLYRATVPLI"
     gene            complement(1867..3024)
                     /locus_tag="PKM23_05625"
     CDS             complement(1867..3024)
                     /locus_tag="PKM23_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNY90989.1"
                     /translation="MKNFTYFNPVRVYFGKGTIARLKESIPAGARVMMTWGGGSIRQN
                     GVYEQVASALKNHPVAEFGGIEPNPRFETLMRAVAAARSEKIDFLLSVGGGSVLDGTK
                     FIAAAVPFPGPEPWDLLSKHTPVRAALPIGCVLTLPATGSEMNGTAVISREATREKLA
                     FGSPLLFPRFSVLDPESTLSLPPRQTANGVVDAFVHVLEQYLTYPAAAPLQDRQAEAI
                     LLTLIECGPRLLASPDSYDERATLMWCATQALNGLIGCGVPQDWATHAIGHELTAAYG
                     LDHAQTLAVVLPALLRHQKQQKGEKLLQYGERIWGISSGSEEQRMEEAIAQTEAFFRS
                     LGVRTRLAEYDIPAEGVRGIGARIEARDGKIGERRDIGAEQIDAILQACTK"
     gene            complement(3178..5553)
                     /locus_tag="PKM23_05630"
     CDS             complement(3178..5553)
                     /locus_tag="PKM23_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY90990.1"
                     /translation="MGILLAFWGTLSLAADIGRELDPVIVKGQSLPALLGKDTIGLRV
                     YAYKSGIWSPVPFQLDEVVAVRDSGYYSDFIKKLDPIDELVFMAKDLGDQAGERDWAP
                     DENAKQNPRYEIAVANPVTGATGYLYVYWSTDLAKSDLSYISYADETVTTAKYVAGQD
                     KAAAGGLLTNLTIPASAGGDGIDLLDEQRLRLQTTFDHFATRALTIYIREKWAKRDVI
                     ELDTGTDVGEINVNAGHSHYKYTAGPIRVIRTNYLKVRIWGNAKIQSIAEFSFDQTLL
                     LPVGYKFYPDYYEMPFDSIVIDLSKSLSGMDKYGVSLTNSRILLATAPNTNAKGMRYF
                     TPRFPIEADRIAGLKIDQSSDEAAYGKVATLKSGDWPGRHWYGLVSDNLPPASPAAKA
                     TLFTMVDLRGALPAMTSLVRYSDYDITDGSPFVYGLNGIQLQAESGLPSAIPLILTLR
                     QYVFPQVYNYQGLQELFDRYKTPVTFSATRQMFDIVPPGTITDLQIAGRADSTLTLVW
                     TAVGDDCTSVRAATEYYLKYSTAKPASNRDWEWWGNAAHYTPPVPAAPGTTQSFTITG
                     LQPGMQYYFGLRAIDEAGNASPSIAMITSVTTPVELSSFTAAVRDNRVELNWRTASES
                     NNYGFAVERRFDKESAWQETVFIKGNGTTSSARSYSWIDDSARPGTIQYRLKQIDTDG
                     SYSYSEPVTVVLSAPAVWALAQNYPNPFNPATTISYEVPANSAGRIDLTIYDLLGRRI
                     RTLVQGEAQAGYYRIDWDGRDDRGLAASSGVYMYILSAERTHIVRKMIKLQ"
     gene            complement(5633..5971)
                     /locus_tag="PKM23_05635"
     CDS             complement(5633..5971)
                     /locus_tag="PKM23_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY90991.1"
                     /translation="MALHEEMYGDVAVLELKGKLMGGPETSEIHEKVKELIGQGAKKV
                     VADVEKVSWMNSTGLGALIGAMSSLRNAGGDLKLARITENVENLFVITKLVTIFDTFD
                     TVEEAVTAFK"
     gene            complement(6050..6763)
                     /locus_tag="PKM23_05640"
     CDS             complement(6050..6763)
                     /locus_tag="PKM23_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY90992.1"
                     /translation="MAEKIRPFLAINRQWMIKRILVSYFRAKNTFANIDREVDAGREI
                     TFDNILKLSDGLFKAKEEMHLLFQRARADGQRAEYEKLEPTGLEVDLINNVGLLFHKV
                     MVVREQVYILEHYADESADRTLTRANLDVYMDKMRALFNQGLEIISGLMRDYRDNEVL
                     LYYMITNEHYIRFVFGEELDALLARMHGTEIDRAYLLAGKFCLDSGWEEAGRKCLLEA
                     MRVNPKNREAKNLLQHDTN"
     gene            complement(6830..9397)
                     /locus_tag="PKM23_05645"
     CDS             complement(6830..9397)
                     /locus_tag="PKM23_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNY90993.1"
                     /translation="MTHPFAHRATHLALLLAAGLILLVPDRLPAQASAAITSPENFLG
                     FTPGDDFRLADTGTILAYFRLLDRQSPRLQLQRLGQSTQGRTLWMAVISSPENLARQD
                     DLIQLQAQLSDPRGLVSTQARRLMHDARSVVCITCSIHPIEIGSSQMSMNLAYRLVAE
                     NSEEIKTILDNVVLLLVPTQNPDGLDAVVEWYKKYRDTPFAGGPLPFLDHPYSGHDIN
                     RDWVHLTQKETRLTVEKLFNVWRPHVHFDLHQMGYTGPRMFLPPYRDPHDENIPSLLQ
                     AQASAIGMAIGAAMTAEGLAGVAHNVHFDAFASSRAYVFYHGGIRLLGEIASARLATS
                     LAITPDELKGDEEFVPRQRSWRQPLPWGGGTWRFGDVVEYAQASSLALLRLAARDRDE
                     WQQTRWQVQSEAVREGDDREVFVLPPRQFDPLALHELLSLLQLGQVEIYRAEEKVTVS
                     GTLYPEGSYIVPVAQPYGAWARTLLQTRLYIPGGTGADQPYDVSSYNLAQGLGVKVIE
                     GSGEIFGKIERIVKLKPPAGGVKDASQRGGFFLDYRSQAANKVINRLLAAGIPVYWLR
                     ETQRTTREVWPPGSCWIVTDSVRLLHDLAQIYNVTFVAGRLASPQPAWRLKTPRIGLY
                     TSYLAEVDEGWTRFVLDMNDFKYDRLINPTLQRGDLRRQYDVIILPSQSLGELMEGAP
                     AGRLPDLFSGGIGESGSAELRRFVEGGGTLIALNRACALPLQWELPVRDVTAALGQQE
                     LFVPGAFLRATFQPGHPLGYGMPTDLNLFFHDSPAFVVESGEAALIYPNERLLQDGYA
                     RGEGHLTGRAALAWVARGAGHVELIGFRPQFRAQTRGTFKVLFNGLYLSTASETDCP"
     gene            complement(9476..10933)
                     /locus_tag="PKM23_05650"
     CDS             complement(9476..10933)
                     /locus_tag="PKM23_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HNY90994.1"
                     /translation="MSGNLVTYLRERREPVTAAELAAEVLKLQNVPEAVAARLIASLL
                     DREKEVDRVGEDSFVYRAAPASGLALPSWLICCVLPERASHWRDWQGAACTLLQGGRR
                     QRLEGSGSRDGLGWPERLRSLLRSIDEAEAGIPLVFSGFGNQISLFRQAWLDLLNRPL
                     DRPLLSMRLIAAGLFSGQRIGSAEEMAAALGTPAWSDAEIGQMNEQLLLFWEHLLAEL
                     AVRGIDSPLELAASMAAAAPEIDLSPYAFDAAFLQALPATPGVYLMRDRAGAVIYVGK
                     AKNVAQRVKSYFAPGVEPDAKLTEIRGRLHRLEIREVGSELEALLLEQRMIASCDPPI
                     NRQIAVQPRPHRRRSRHARILILPAVAPEWLRLFFLDPARGSAHFWLARRYGRSSEAP
                     PCHRHWLEVDGRSVEALLRSRSALRRAVQDFFWNEGAAGDPAAAEIAWSWLGEQTEAI
                     HGIDMRLAAAPAEAVRLIEEYRQRLGEWHEKIIFT"
     gene            complement(11080..>12100)
                     /locus_tag="PKM23_05655"
     CDS             complement(11080..>12100)
                     /locus_tag="PKM23_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNY90995.1"
                     /translation="YYNKLAGMTRITTETGAGQWGGALAFSCALLGLECKVYMVRVSF
                     DQKPFRRLMMEAWGAQCVASPSTDTRAGRQMLQDNPDTPGSLGGAISEAIEDAVSDPT
                     GRTRYSLGSVLNHVLLFQTVIGLEAKQQLAKAGEKKVDKVIGCVGGGSNFAGIAFPFL
                     LDKMNGADMEIIPVEPSSCPTMTRAPFGYDHGDLAEMTPLLAMHSLGHKFVPPPIHAG
                     GLRYHGVAPQVSHAINLGLLSPRAVDQLEVYDAGVAFARSEGIIPAPESCHAIAAAIQ
                     EAEKAKEEGVERVILFNLSGHGLMDLNGYDRYFRGELMNYPLPEEEMARSIASLGKYP
                     KPAMA"
BASE COUNT         2332 a         3693 c         3845 g         2230 t
ORIGIN      
        1 cgcagcgtag gagaggaaga atttgcggtg gctgttgagg acgcccaggc accaggagga
       61 gagaacgagc agacctgcgc ccgggaagag gatgcgcacc agctggatgg tcaattcgcg
      121 cttggctccc gagaaaccgg gggcgataag agcgatgagc cagggggtga ggagaacacc
      181 ggcgaggacg ctcagggcga ccaccaggct gaggatggcc gcgaccgccc ccgccacccg
      241 cccggaatcc tcctcccgct cctcggcgag cagccgggca tagaccggaa tgaaggaggc
      301 ggagagggtg ccctccccga agaggttctg cagaaagttg gggatgcgga aggcggcttt
      361 aaaggcatcg gcggcgtccg aactgccgaa aaaatgggcg aagacgcggt cgcggatcag
      421 tccggcaatg cggctgaaaa agacccctgc ggccaccaga atggccccgc cgccggagtg
      481 cgccgcgcgc ggctgcacct ccaccgctcc cccggtttcg gaatcgagat ctgacaacga
      541 ctgcttcctg taatttaaat tgagaatcag atgaggggca ccgtggcgcg gtaaagcagg
      601 gccccgctgc tgctgtgcag ccgccagggc gaattcgcgg cagcaaggag ggcatcaccg
      661 cgcctgagcg ccaactcctc cccctccgcc ctgatcgacg cctcgccctc gagcgcgatg
      721 agaatgtcgg gcccatggcc gcttccgctc gcatggaccc cgcggggttc gagctcgatg
      781 cgggagagca gaaaatcatc gaccggcgcg ggatagaggc gctctcctcc ctctgccacc
      841 acaccggtga ggatctccgg ccggccgtca tcgaagcgaa tggtcttcaa cagctccgcc
      901 acatccacat gcttgggcgt caaccccccg cgcaggacat tgtccgagtt ggccatcaac
      961 tcgacattga ccccctcgag gtaggcgtgc ggaaccccgg ccccctgaaa ggtcccctgc
     1021 ccgggctgca gctcgaccag gttcatcaga tagatcgaaa agatcccccg gtcgatgtgg
     1081 ccttccggca aagggaagga acgggcggcc ttgactgccc aataatgggg cgagctcttg
     1141 tgcagactcc ccgcgtcgta gaggggctcg agatgggcca gcagcccgcc cagaatccgg
     1201 tcggcctcat cctggggcat ggtcatgata aaggcgtaga ggcggcggag caactccgcc
     1261 cggccggccg gatcctcatc cacctcaagg aggtggaggg gaaaccaggg ggacagcccg
     1321 gtaaaatcgg ggatgccggc cagcacatcg gcgatctcct cgagggggcg aaagccgtgc
     1381 agcatccaga actcgctgag ggcgacatgg acctcgggct tgtgattgcg atccttgtag
     1441 ttgcgccgag gatccgtaag gggcataccg gccgcctcct ccgctgcgca gccagcctcc
     1501 gactgcgcgc gcgaggggtg cacctggatc gaaagcatct cgcgggcatc gaggaccttg
     1561 aagagaaagg gtagcccggc gccaaaccga gccaggacgg cggatccgag gatccgctcc
     1621 ccctcccgcc gcagcagttc aaccaggtcg atccggccgc cttccacctc cacctcgctc
     1681 ggggcctggg gatgactccc cagccagagt tcggcgaagg ggcgcttttc gtcattgcgc
     1741 acccccagca ggccgggaat gcaggcgtag cctccccagg gatagtgcat gacgcggcca
     1801 tgcaggggat agagcgattg agcggggcgc cggcttgtca tttgggtttt catcgggcgg
     1861 acgggatcat ttggtgcagg cctgcaggat ggcgtcgatc tgctccgcgc cgatatcccg
     1921 gcgctcgccg atcttgccgt cgcgggcttc aatgcgcgcc ccgatgcccc gcacgccctc
     1981 agccgggatg tcatactccg ccaggcgggt cctgacgccg agggagcgaa aaaaggcctc
     2041 ggtttgggcg atagcttcct ccatgcgctg ctcctcactg ccgctgctga ttccccagat
     2101 ccgctcgcca tactgcagca gcttctcccc tttctgctgt ttttgatgcc tcagcagcgc
     2161 cggcagcacc accgcaagag tctgcgcatg gtcgaggcca taggcggcgg tgagttcatg
     2221 gccgatagcg tgggtcgccc agtcctgcgg cacgccgcag ccgatcaggc cgttgagggc
     2281 ctgggtggcg caccacatca gggtggcgcg ttcgtcgtaa ctgtccgggc tggcgagaag
     2341 gcgcggcccg cactcgatca atgtgagcaa aatggcttcg gcctggcgat cctgcagggg
     2401 cgccgcagcc ggataggtga gatactgttc cagcacatgc acaaaggcat caacgacacc
     2461 gttggcggtc tgccgcggcg gcaggctgag ggtgctctcc ggatcgagaa cggagaagcg
     2521 cggaaaaagc agcggcgagc cgaaggcaag cttttccctc gttgcctcgc gcgaaatcac
     2581 cgccgtgccg ttcatctcgg agcccgtcgc cggcagggtc aggacgcagc cgatgggcag
     2641 ggcggcgcgg accggggtgt gcttgctgag cagatcccag ggctccggac cgggaaaggg
     2701 cacggcggcg gcgatgaatt tggtgccatc cagaaccgac ccgccgccga cagaaagcag
     2761 aaagtcgatt ttttcgctgc gcgcggccgc gaccgcacgc atgagggttt cgaaacgggg
     2821 attgggttcg atgccgccga attcggccac gggatggttt ttcagggcgg aggccacctg
     2881 ctcataaacc ccgttctgcc tgatcgagcc accgccccag gtcatcatga cccgcgcgcc
     2941 ggcaggaatg gactccttca gcctggcgat cgtgcccttg ccgaagtaga cgcgcaccgg
     3001 attgaaatag gtaaaatttt tcaaaaagcc tccagctgga tttctggtga cgcccggcgg
     3061 caggggcaga taattggggc tgctgcggcc gggggcggga caaaaaaatg cgacgcacgt
     3121 tcggcatgcg tcgcacggct ttgcatcatg acaataacaa aaatgactgg agcaggatca
     3181 ttgcagcttg atcatcttgc gcacgatgtg ggtgcgctcc gcactcagga tgtacatata
     3241 gacgcctgaa ctggcggcca gtccccggtc atcgcgtccg tcccaatcga tgcgataata
     3301 gcccgcctgc gcctccccct gcaccagagt gcggatgcgg cggccgagga gatcgtagat
     3361 ggtcaaatcg atccgcccgg cactgttggc cggcacctcg tagctgatgg tggtggccgg
     3421 attgaaggga ttgggataat tctgcgcaag ggcccagacc gccggggcgc tcagaacgac
     3481 tgtaacgggc tcggagtagc tgtagctgcc gtcggtgtcg atctgtttga gccgatactg
     3541 gatggtcccg ggtctggcgc tgtcatcgat ccagctgtag ctgcgggcgc tgctggtggt
     3601 gccgttgccc ttgatgaaga cggtctcctg ccaggcgctc tctttatcaa agcggcgttc
     3661 cacggcgaaa ccgtagttat tgctctcgct ggcggttcgc cagttgagct cgacgcggtt
     3721 gtcgcgtact gctgcggtga aggatgagag ttcgaccggg gtggtgacgc tggtgatcat
     3781 cgcaatcgag ggcgaggcgt tgcctgcctc atcgatggcg cgcaagccaa agtagtactg
     3841 catgccgggc tgaaggccgg tgatagtgaa agattgagtc gtgcccggag ccgccggcac
     3901 gggaggcgtg taatgcgccg cgttgcccca ccattcccag tcgcggttac tcgcgggttt
     3961 ggcggtgctg tatttgagat agtactccgt cgctgcccgg acgctggtgc agtcgtcgcc
     4021 gacggccgtc cagaccaggg tgagcgtcga atctgcccgt ccggcgatct ggagatcggt
     4081 gatggtcccc ggtggcacga tatcgaacat ctgccgagtg gcggaaaaag tcaccggtgt
     4141 cttgtagcga tcaaagagct cctgcaatcc ctggtagttg tacacttgag gaaaaacata
     4201 ctggcgcagg gtgaggatca gggggatagc cgatggcaga ccactctcag cctgcagctg
     4261 gatgccgttc agaccgtaaa caaagggcga gccatccgta atatcataat ccgaataacg
     4321 caccagggag gtcatcgccg gcagggcgcc tctcagatcc accatagtga agagggtggc
     4381 cttcgcggcc ggactggcgg ggggcagatt atccgagacc aggccgtacc aatgacggcc
     4441 gggccagtcg ccagatttca atgtcgccac tttgccataa gctgcctcat cgctgctctg
     4501 gtcgatcttg agccccgcta ttctgtccgc ttcaatggga aaccgcggag tgaaatagcg
     4561 catgcccttg gcattcgtat tcggggcggt agcgaggagg attcttgagt tggtgagcga
     4621 aacgccgtat ttatccatgc cgctcagcga tttgctcagg tcgattacga tgctgtcgaa
     4681 gggcatttcg taataatcgg gatagaactt gtagcccacc ggcagcaaga gggtttgatc
     4741 gaaagagaac tccgcgatgg attgaatctt ggcattgccc cagatgcgca ccttcagata
     4801 gttggtgcgg atgacccgga tgggcccagc ggtgtatttg tagtggctgt ggccggcgtt
     4861 gacattgatc tcgccgacgt ccgtgcctgt gtccagttca atcacatctc ttttagccca
     4921 tttctcgcgg atataaatag tcagcgccct ggtcgcaaaa tgatcgaacg tcgtctgcag
     4981 gcgcagccgc tgttcatcga ggaggtcgat gccgtcgccc ccggcgctgg ccggaatggt
     5041 cagattggtg agcaggccgc ccgcagccgc cttatcctgg ccagccacgt atttggcggt
     5101 cgtgacggtt tcgtcggcgt aagagatgta ggagagatcg ctcttggcca gatcggtcga
     5161 ccagtaaaca tagagatagc ccgtggcgcc ggtaaccggg ttggctaccg cgatttcata
     5221 gcgcggattc tgttttgcgt tttcatcggg agcccaatcc cgctcccccg cctgatctcc
     5281 cagatctttg gccatgaaga ccagctcatc gatgggatcc agctttttaa tgaaatcgga
     5341 atagtagccg ctgtcgcgca ccgccacgac ctcatcgagc tggaagggca ccggcgacca
     5401 gataccggat ttatacgcgt agacgcggag tccgatggta tccttgccga gcaaagcggg
     5461 aaggctttgt cccttcacga tcaccggatc cagttcacgg ccgatatctg ctgccaatga
     5521 aagagtgccc cagaaagcca gaaggattcc catccagggc actcgtctca ggcaccatcc
     5581 cctgttgatt gatggcataa cacacctccc gcaggatcac ctgcagcacc acctatttga
     5641 aggctgtgac cgcttcctcg acggtgtcga aggtatcaaa tatggtgacg agcttggtga
     5701 tcacaaacaa attctcgaca ttctccgtga tacgggccag cttgagatcg ccgccggcat
     5761 tgcggaggga actcatggca ccgatcaggg cacccaatcc ggtgctgttc atccagctca
     5821 ccttttccac atcagcgacc acttttttgg cgccctggcc gatcagttcc ttgacctttt
     5881 cgtggatttc gctggtctcc gggcctccca tcagcttgcc cttgagctcg agcactgcaa
     5941 cgtccccgta catttcttca tgcaacgcca taataacctc ctgaggatca cggttcgaag
     6001 aactgttgtg gttttttaaa aatagggctt tttacataat aaagcaacgc taatttgtgt
     6061 catgctgcaa caagtttttg gcttcgcggt ttttgggatt gacgcgcatg gcctcgagca
     6121 ggcactttct gccggcctcc tcccagccgc tgtcgaggca gaatttcccc gccagcaggt
     6181 aggcgcggtc gatctccgtg ccatgcatac gcgccagcag ggcatcgagt tcctcaccga
     6241 agacaaaacg gatataatgt tcgttcgtga tcatgtagta gagcagcact tcattgtcgc
     6301 gataatctct catcaagccg gagatgatct ccaggccctg gttaaagaga gcgcgcatct
     6361 tgtccatgta aacatcgaga ttggcgcgtg tgagggttcg gtccgcactc tcatcggcat
     6421 agtgctcaag tatatagacc tgctcgcgca ccaccatcac cttgtggaaa aggagcccga
     6481 cgttgttgat caggtcgacc tccagtccgg tcggttccag cttctcgtac tctgcacgct
     6541 gcccatccgc ccgggcgcgc tggaagagca gatgcatctc ctccttggcc ttgaaaagtc
     6601 catcggagag cttgagaata ttgtcgaagg tgatctcacg gccggcatcc acctcacggt
     6661 cgatattggc gaaggtgttc ttcgcgcgaa aatagctgac cagtatccgc ttgatcatcc
     6721 actgccggtt gatggccaga aacggcctga tcttttcagc catagacctc cctattatgc
     6781 tgctgggtat gtactacaac aatggcacca aaggattgca ttcccgcgtt cagggacaat
     6841 cggtctcact tgcggtgctc agatagagac cgttgaacag caccttgaag gtgccgcgcg
     6901 tctgggccct gaactggggt cgaaagccga tgagctcgac gtgtcccgct cccctggcca
     6961 cccaggccag ggccgcccgg ccggtcaggt gcccctcccc ccgggcatag ccatcctgaa
     7021 gcagtcgctc gttcggatag atcagcgccg cctcgccgct ctcgaccacg aaagcggggc
     7081 tgtcatggaa aaagaggttc aggtcggtgg gcatgccata gcccagggga tgaccgggct
     7141 gaaaggtcgc gcgcaggaag gcccccggca cgaagagctc ctgctgccca agggcagcgg
     7201 tgacatcgcg aaccggcagc tcccactgca gcggcagggc acaggcccgg ttgagcgcaa
     7261 tcaaggtccc gccaccttca acaaaccggc gcagttcggc cgaaccggat tcgccgatgc
     7321 ccccgctgaa caggtcgggc agccgtcccg ccggagcccc ctccatcagc tccccgagac
     7381 tctgcgaggg caggatgatc acatcgtact ggcggcgcag atcaccgcgc tggagggtgg
     7441 gattgatcag ccggtcgtat ttgaaatcat tcatatccag gacgaaacgg gtccagcctt
     7501 catcgacctc cgccagatag ctggtataaa gtccgatgcg cggcgtcttc agcctccagg
     7561 ccggctgcgg cgatgccaga cggccggcga caaaggtcac attgtagatt tgcgccagat
     7621 cgtgcagcag ccggacggaa tcggtgacga tccagcagct gccggggggc cagacctcgc
     7681 gggtggtgcg ctgggtctcc ctgagccagt acaccggaat gccggccgcc agcagccgat
     7741 tgatcacctt gttggccgcc tggctgcggt aatcgagaaa aaagcccccc cgttgggacg
     7801 cgtcctttac acccccggcc gggggtttta gcttgacgat cctttcgatc ttcccgaaaa
     7861 tctctccgct cccctcgatc accttgaccc ccagtccctg ggcaaggttg tagctgctca
     7921 catcataggg ttgatcggct ccggttccac ccggaatata gaggcgggtc tgcaggaggg
     7981 tgcgcgccca ggcgccgtaa ggctgcgcga cgggaacaat ataactccct tccggataga
     8041 gggtcccgga aacagtcacc ttctcttcag cgcgataaat ctcgacctgg ccgagctgaa
     8101 gcagggagag cagctcatga agggcgaggg gatcgaactg ccgcggcggc aaaacaaaga
     8161 cctcgcggtc atccccctcg cgcacggcct cggactgcac ctgccatctc gtctgctgcc
     8221 actcatcccg atctcgcgcc gccagccgca gcagggccag gctcgacgct tgcgcatatt
     8281 cgacgacatc gccgaagcgc caggtgccgc cgccccaggg caggggctgc cgccaggagc
     8341 gctgacgagg tacgaactcc tcatccccct tgagctcatc gggggtgatg gccagagagg
     8401 tggcaaggcg ggcggaggcg atctccccga ggagacgaat accgccatga taaaaaacgt
     8461 aggcccggct cgaggcaaag gcgtcgaaat ggacattatg agcgaccccg gcgaggcctt
     8521 cggcggtcat tgcggccccg atcgccatgc cgatggccga ggcctgggcc tgcagcagag
     8581 aggggatgtt ctcgtcgtgg ggatccctgt aaggaggcag aaacatgcgc ggccccgtat
     8641 agcccatctg gtgcagatcg aaatggacgt gcggccgcca gacattgaag agcttctcca
     8701 ccgtcagccg cgtctctttc tgggtgagat ggacccagtc gcgattgata tcatggccgc
     8761 tgtagggatg gtcgaggaag ggaagcggcc caccggcgaa gggggtatcg cggtactttt
     8821 tataccattc gaccacagca tcgagaccgt cgggattctg ggtcggaacg agcagcagga
     8881 cgacattgtc cagaatggtt ttaatctcct cgctgttctc cgccaccagg cgataggcca
     8941 gattcatcga catctgcgag gagccgattt cgatcggatg gattgagcag gtgatgcaga
     9001 ccacggagcg ggcatcgtgc atcagccggc gcgcctgggt actgaccagg ccgcgcggat
     9061 cggagagctg cgcctgcagc tgaatcaggt cgtcctgccg ggcgaggttc tccggggatg
     9121 agatgaccgc catccacaag gtgcgtccct gcgtggactg gccgaggcgc tgaagctgca
     9181 ggcgggggct ctggcggtcg agcaagcgga aataggcgag gatggtcccg gtgtcggcga
     9241 ggcggaaatc atcgcccggc gtgaatccga gaaagttctc cggcgaggtg atggcggcgg
     9301 aggcctgcgc aggcagccgg tccggaacca gcaggatcag tcccgccgcc agcagaagcg
     9361 ccaggtgcgt ggctctatgt gcaaaggggt gtgtcatcgt ctcgtcgaaa ttctaactga
     9421 ggatccccgc aggctcgcga ttgcgcgggg tgttgatctg ctgcggaggg agggatcagg
     9481 tgaaaatgat cttttcgtgc cactccccca gtctttgacg atattcctcg atcagccgca
     9541 ccgcctcggc gggtgcggct gccaggcgca tgtcgatgcc gtggatggcc tccgtctgtt
     9601 ctccaagcca gctccaggcg atctccgctg cagccgggtc tccggccgcc ccctcgttcc
     9661 agaaaaagtc ttgcacggcc ctgcgcaggg cgctccttga acgcagaagg gcctctacac
     9721 tgcggccatc cacctccagc cagtggcgat ggcagggtgg cgcctcggag ctccggccgt
     9781 agcggcgtgc cagccaaaag tgggcggatc cgcgcgccgg atcgaggaag aaaaggcgca
     9841 gccattctgg cgccacagcc ggcaggatga ggatgcgggc atggcggctc cggcgccggt
     9901 gcggccgcgg ctgcactgcg atctggcggt tgatgggggg atcgcaggag gcgatcatgc
     9961 gctgttccag cagcagggcc tccagctccg agcccacctc cctgatctcg aggcggtgga
    10021 gacggccgcg gatctccgtc agcttggcat ccggctcgac cccgggtgcg aaatagcttt
    10081 taacgcgctg ggcgacattt ttcgccttgc ccacatagat cactgcgccc gcccggtcgc
    10141 gcatcaggta gactccgggc gtggccggca gggcctgtaa aaaggcggcg tcaaatgcgt
    10201 agggggagag atcgatctcc ggcgctgcag cagccatgct cgcggccagc tccagtggcg
    10261 agtcgatgcc ccgcaccgcc agctctgcca gtagatgctc ccagaagaga aggagctgtt
    10321 cgttcatctg gccgatctcc gcgtcgctcc aggcgggcgt tcccagcgcg gccgccatct
    10381 cctcggcact gccgatgcgc tgccccgaaa aaagtccggc tgcaatcagc cgcatggaga
    10441 ggagagggcg gtcaagaggc cgattcagca ggtcgagcca ggcctggcgg aagagggaga
    10501 tctggttgcc aaagccggaa aaaaccagcg ggatgccggc ttctgcctca tcgatgctcc
    10561 gcagcaggct gcgcagacgc tccggccacc ccaggccatc cctgctgccg ctcccttcga
    10621 gccgctgccg ccggccgccc tgcagcaggg tgcaggcagc accctgccag tcgcgccaat
    10681 ggctggcacg ttccggcagg acgcagcaaa tcagccacga tggcagggct agtccgcttg
    10741 cgggtgcagc ccggtagaca aacgagtcct cgccgacgcg gtcgacctcc ttttctctgt
    10801 ccaggaggct ggcgatgagc ctggcagcga ccgcctcggg cacattctgc agcttgagca
    10861 cctcggccgc caactcggca gccgtaaccg gttcccggcg ctcgcgcaaa taggtgacaa
    10921 gattgccgct catcgctcct gcactgctga tcggtctgga cgattcatta ttaaaatacg
    10981 atttttcggt gagggaatga aatccttttt tccttttcac cgccaggcgg agaaaaaaaa
    11041 gccgggtccg gctgcccgga cccggcttgt gccgggggat tatgccatgg caggcttggg
    11101 atacttgccc agcgaggcga tcgatctggc catctcctct tcgggcaagg gatagttcat
    11161 cagctcgccg cggaaatagc ggtcatagcc gttcaggtcc atgaggccgt ggccgctgag
    11221 gttgaagagg atcacgcgct ccaccccctc ctccttcgcc ttttcggcct cctggatcgc
    11281 ggcagcgatg gcgtggcagc tctcgggcgc cggaataatg ccctcggagc gggcgaaggc
    11341 gacaccggcg tcatagacct cgagctggtc cactgcgcgc ggcgaaagca ggccgaggtt
    11401 gatggcatgg ctgacctggg gtgcgacgcc gtgatagcgc agcccgcccg catggatggg
    11461 gggcggcacg aacttgtggc cgagggagtg catggccagc aggggggtca tctccgcgag
    11521 atcgccgtgg tcatagccga atggagcgcg ggtcatggtc gggcaggagc tcggctcgac
    11581 cgggataatc tccatatcgg ccccgttcat cttgtcgagc agaaagggaa aagcgatgcc
    11641 ggcgaagttg ctgccgccgc cgacgcagcc gataaccttg tccacttttt tctcgccggc
    11701 cttggccagc tgctgcttcg cctccagccc gatcaccgtc tgaaagagga ggacatggtt
    11761 gagcacgctg ccgagggaat agcgggtgcg gccggtggga tcgctgaccg catcctcgat
    11821 cgcctcgctg atcgcgccgc ccaggctgcc cggggtatcg ggattatcct gcagcatctg
    11881 acggccggcc ctggtgtcgg tgctggggct ggcgacgcac tgcgcacccc aggcctccat
    11941 catcagacgg cggaagggct tctgatcgaa actgacccgc accatgtaaa ccttgcactc
    12001 cagcccgagc agggcgcagg agaaagccag ggcgccgccc cactgacccg cgccggtctc
    12061 ggtggtgatc cgggtcatgc cggccaactt gttgtagtag
//
LOCUS       DAOPWU010000074        12083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5387_length_12083_cov_4.392002, whole genome shotgun sequence.
ACCESSION   DAOPWU010000074 DAOPWU010000000
VERSION     DAOPWU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12083
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5387_length_12083_cov_4.392002"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PKM23_05660"
     CDS             <1..871
                     /locus_tag="PKM23_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNY90996.1"
                     /translation="APEWAAPDRNVNYDPAKHPERWQKFKDYTYAQIKALMSRYGRID
                     ILWLDGGWVRPQSTITDEVRSWAGMKPWDQDIDMPGIAAMARGHQPGVLIVDRTVQGP
                     FENYRTPEQQVPDQPLDYPWETCMTMGDQWSFKATDRCKPARQLIHLLIEIVAKGGNF
                     LLNIGPEPTGEWTDEAYARLEEIGAWMGVNGEAIYGSHPLPPFRNGKICYTQGGDGTR
                     YAFYLADEGEHEPPAELLLPALSAGPGTKVTLLGYGPGLRWQAVKEGIRVNIPQELRA
                     GMPCAYAWTFTIH"
     gene            939..2969
                     /locus_tag="PKM23_05665"
     CDS             939..2969
                     /locus_tag="PKM23_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNY90997.1"
                     /translation="MIGLVLAGGLARAETKKAFDARMRWWRDAKFGMFIHWGPYAVLG
                     GVYKGQNSAAAEWIMNEDNIPAAEYEEYARQFNPVQFNARAWVDLAKAGGVKYIIFTS
                     KHHDGFSMWDSKVSGYDIAEFTPFKRDVLKELAEACKQAGIRLGFYYSIMDWHHPDAA
                     GERFAAYRDAYLKPQLQELLTGYGKVGVLWFDGEWIDDWSEGQGAELYSFVRRLQPEI
                     IVNNRVGKGRNGMQGMSLDKSSAGDFGTPEQEILAQGGAAMDWEACMTTNDSWGFKKS
                     DNNWKSPEALIHNLIDIAAKGGNYLLNLGPDAEGLIPEAGQERFREIGKWLKVNGAAI
                     YGTRPLPEYREGENIRYTRSLDGKTIYATVLEWPGKSLRLKYIEPKKKSKIYLLGRKS
                     ALTWKANSRGEVVITLPALLQRAANRPCRHAWVFKITGRQLRVAAAPLISTASKTGVV
                     RSLFTDSEQITISAPSDGAEVHYTLDGRTPSQNSPVYTAPITLTEAAEIQAVALEKGK
                     VISPVTTAQFSRTTRIKRLTLEKSPSVKYPGWGELGLVDGRQGGTDYHESDWIGFEGE
                     SLDATIDLGEQKSISSIRLSCLEDQNSWIFLPSSIKLLTSTDGATFVQTAEWKGETRP
                     AGTAATRETALIIMPKSCRYLRILASNVGVCPSWHKGAGGKAWLFIDEIIIN"
     gene            2979..4364
                     /locus_tag="PKM23_05670"
     CDS             2979..4364
                     /locus_tag="PKM23_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY90998.1"
                     /translation="MAAGDHPDKDTQGVDRRSFFKTGVLAGLGALTAASTLNRCDATA
                     SGRGPAPAQLFSVAPLKKVRIGMVGVGLQGTSHVRNFLGIPEAQITAVCDIRPERVAA
                     AQKLITAAGHPEPRGYSRGSTDFLRLCDEEELDLVFTATPWEWHVPVCVAAMKSGKHA
                     ATEVPAAVTVDECWQLVETAEKHHKHCVMMENCCYDREELLILNLVRKGMLGEIINAE
                     AGYLHDLRNVKFNNENEAVWRLPHSIKRDGNLYPTHGLGPVAQCMNINRGDQFDYLVS
                     MSSVSRGLNLYASRLYGAEDPRATQRYALGDVNTTLIRTVLGKTITLIHDTSTPRPYT
                     RINRVQGTRGLAEKWPHRVYIEGKSEPHRWDDLADYYQEHDHPLWRELQQRAAGSGHG
                     GMDFIECYRLIHCLLNGQPMDMDVYDAASWSCISELSERSVARRGRPMDVPDFTRGRW
                     KNWPQLGIISA"
     gene            4476..6854
                     /locus_tag="PKM23_05675"
     CDS             4476..6854
                     /locus_tag="PKM23_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNY90999.1"
                     /translation="MEIHIMTSCARILIPALLAALCGCAGAPRMQAPSAQTGPALIPL
                     PVKMTARPGHFRLDGSTVTASSGEAEEVAEYVAQLIRRGTGWQLARAAGVPPDSNAIV
                     LELTGSPAASDAEGYTLEITPQRILIKAARPAGLFYGVQTLRQLWPAAWEGPAPAVQA
                     EPARIPCLVINDQPRYSWRGGHLDVCRHFFPIEFIYRYIDYLAMNKMNTFHWHLTDDQ
                     GWRIEIKKYPLLTEVGGWRVDRSGDHWNKRAPQQPGEKATYGGYYSQEEIRAVVEYAS
                     SRFITIVPEIEMPAHAGAALAAYPRFSCTGGPFTVPPGGVWPIVNLYCAGDDTTFSFL
                     QDILDEVIGLFPGDYIHIGGDEADKSEWKKCPKCQARIRTENLGDETGLQSYMIRRME
                     GYLHSRGKRIIGWDEILEGGLAPRAAVMSWRGMQGGIDAARADHDVVMTPTDYCYFDY
                     YQGPADFEPLSIGGYIPIETVYAFEPTPASLDEKQAKHILGCQANLWTEFIATPEHAE
                     YMLFPRLLAMAEVAWSPKSSRGWDHFLDRLTAFLPRLEAMGVNYSRSLYSVKPVLERS
                     SDGRSALVALKSPAPFAVIHYTLDGAEPTAASPRYESPITLQGSSTIKAGLFLDGALQ
                     GRIVAVPFLVHKASGQKVTYTHPWSDRYQAAGPTALVDGLTGSKNYNDGSWQGFEQHD
                     LEVVIDLGREMEIRRVSSSYLEDFGSWIFAPREVSYAFSDKGKEFQTLAVLPGGGMQD
                     GNEITIHHFGRDFSNLRARYLKVTAKNIGVCPPDHAGAGGKAWLFVDEIIVE"
     gene            6860..9718
                     /locus_tag="PKM23_05680"
     CDS             6860..9718
                     /locus_tag="PKM23_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNY91000.1"
                     /translation="MHRTGFCLLLMLLAGGAAAGADLIPLPASVTTGEGSCTLTASTL
                     LYYDPAVPELQGYAERFAARLRLATGLPLPLQPLPADTSAADALYFTAHGARAEWGRE
                     GYSLEVTTAGGRIRANAAPGFFYGMQTLLQLLPPAVGSPTPVPGTAWRMESVTILDQP
                     RFAWRGVHLDVGRHFFPVEFVKKYVDMIALYKMNTFHWHLTEDQGWRIEIKKYPRLME
                     VGAWRNDGISGPYGGFYTQEEVREIVAYAAERAITVVPEIEMPGHSLAALASYPRLGC
                     TGGPYRVQFSWGVFDDVFCAGRDSTFTFLEDVLTEVMALFPGEYIHIGGDECPKTRWR
                     SCPDCQARIKAEGLANESELQSWFVRRIERFLSANGRRLIGWDEILEGGIAPNATVMS
                     WRGFDGGVEAARSGHDVVMAPREYCYLNFYQGVKSGEPAPYGDYLPIEKVYAFEPVPD
                     VLTSAEAQHVIGAQACLWTEFITESRKAEYMLFPRLCALSEVVWSEPSRRDYADFSLS
                     LQSHFERLEAADINVRIPPSAYFPGTGGDAGFEFRDAGPLSGLLPGGGARIGSDALLW
                     EMTSDSLPSGAALTFAEPQDMRLRIPYYSLSFAYRTNLAPDTLFVGFRDEAGRGFYLQ
                     LLKRHIAATNTWVSVTRALSGFKSDPGFDASRVVRFGFWTNSSRSGARFQFADVYLGV
                     PQLWPVAHSPILFFNGENFNPLFRIKGFENAAGSGVAGAAGMRPGSAALRWLPPADGS
                     PSSVRWRFNETIDLSGVLGADTLKFALKAPAGCDSLQIAFTDAGQRACSLWLTRTEGF
                     FDGQWRTLQLPLTRFTADPDFSAQAVDLFSFFIRSHQPGEELLLSDIWIGQPPASAIS
                     GIEERAVTPAGYSLEQNYPNPFNPLTTIAYHLAERGRVRLTIHNLLGQQITTLIDGVE
                     EGGSHQLYWDASRFASGLYYCRLEVNGFVRTRKMVLVK"
     gene            9730..10755
                     /locus_tag="PKM23_05685"
     CDS             9730..10755
                     /locus_tag="PKM23_05685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY91001.1"
                     /translation="MSGLSIAIIARNEEANIRACLESAAWADEIVLVDACSEDATVTI
                     AREFTDRIFVRKWEGYAAQKEFSLQQCTSPWIFSLDADERIRPELREAMQAVIADPHA
                     LAGYRVGRRSWFLGRWIRHGGWYPGYQLRLFRREAVRMIRRRVHEGFEVEGPIGTLTG
                     DLDHFSHPTIAGSLDKMNRYSTLEAFDRLERKRVHAIDFFTHPFAAFLRKYIAQGGFR
                     DGVNGLVLAWVTAVVKLALYMKIRELQQRPAGETAEAAGGELMRLFDEYQAVLEQYQP
                     AAGKQGRKPGLKAFVFQPAAAFWRHYVRRGRPGGVPGLMQALLAAVYATARELANWEA
                     GLSNTSS"
     gene            10780..>12083
                     /locus_tag="PKM23_05690"
     CDS             10780..>12083
                     /locus_tag="PKM23_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNY91002.1"
                     /translation="MKKLTLFLLFLPVIALTQPPRQKLLFDFGWKFALGHAADPQRDF
                     GYGIGAAFAKAGEAQGAAHPNFSDSLWRSVDLPHDWAVELPFVNHEDPEVKSHGYKPV
                     GRLFPESSVGWYRRAFAIPAADRGRRIRVDFDGVFRDAAVWCNGHYLGRHAGGYTPFS
                     FDLTDYLRYGQRNTLVVRVDASHYEGWFYEGAGIYRHVWLHKYEPLHLAEYGVYVHSQ
                     ISGAAAEVTIETIVENLSPAAAEGRVCVDLLDRDRRLAGRSVLPFAALQPLEATTIRH
                     AITVADPRLWSLEDPHLYTVHTVIEQEGRTIDSLDTPVGLRSLRFDPDRGFFLNGAPV
                     KIQGVCCHQDHGGVGSALPDALQEYRIRLLKAMGCNAYRTSHNPPTPELLDACDRLGM
                     LVMDEHRLMGSSPEILEELETLVKRDRNHPSVILWSLGNEEYV"
BASE COUNT         2197 a         4111 c         3618 g         2157 t
ORIGIN      
        1 ggcccccgag tgggcggcgc cggaccgcaa tgtcaattac gatccggcca aacaccccga
       61 acgctggcaa aaattcaagg attacacgta tgcccagatc aaggcactga tgagccgtta
      121 cggccgtatc gatatcctct ggctcgacgg cggctgggtg cgtccgcaga gcacgatcac
      181 cgacgaggtg cgctcgtggg cggggatgaa accctgggac caggatatcg acatgccggg
      241 catcgccgcc atggcccgcg gccaccagcc gggggtgctg atcgtcgacc gcaccgttca
      301 gggtcccttt gagaattatc gcacccccga gcagcaggtc cccgaccagc ctctcgatta
      361 tccctgggag acttgcatga ccatgggtga ccagtggtcc ttcaaggcga ccgaccgctg
      421 caagccggcg cggcagctga tccacctcct gatcgagatc gtcgccaagg ggggcaattt
      481 cctcctcaat atcggtccgg aaccgaccgg cgagtggacc gacgaggctt acgcgcgcct
      541 ggaggagatc ggcgcctgga tgggggtcaa cggggaggcg atctatggca gtcatcccct
      601 gccgcctttc aggaacggca agatctgcta tacacaggga ggggacggca cccgctatgc
      661 cttttatctt gcggatgagg gagagcatga accgcccgcc gagctgctcc tgcccgctct
      721 ttcggcagga ccagggacga aggtgaccct gctcggctat ggccccggcc tgcgctggca
      781 ggcggtgaaa gaaggtatca gagtcaacat tccgcaggaa ctccgcgccg gcatgccctg
      841 tgcctacgcg tggaccttta caattcacta gagtatggga actgatgtat gcatcttcat
      901 ggggtagagc tatggtgaag agagtattgg ttctcggtat gatcggcctt gtcctcgccg
      961 gcggattggc ccgggcggag acgaaaaagg ccttcgatgc gcgcatgcgc tggtggcgcg
     1021 acgccaaatt cggcatgttc atccactggg ggccctatgc ggtgctgggc ggagtctaca
     1081 aggggcaaaa cagcgccgcc gccgagtgga tcatgaacga ggacaacatc cccgccgccg
     1141 agtatgagga gtacgcccgc cagttcaacc cggtccagtt caatgccagg gcctgggtgg
     1201 acctggccaa agcgggcggc gtgaagtata tcatcttcac ctccaaacac cacgacggct
     1261 tttcgatgtg ggattccaaa gtgagcggct atgacatcgc cgagttcact cccttcaaac
     1321 gggatgtgct caaggaactg gccgaggcct gcaagcaggc cggtatccgc ctcggctttt
     1381 actactcgat tatggactgg caccatcccg acgccgccgg tgagcgcttc gccgcctacc
     1441 gggatgccta tctcaagccc cagctgcagg aactcctcac cggctacggc aaggtcggcg
     1501 tactctggtt cgacggagag tggatcgacg actggagcga aggccagggg gccgaactct
     1561 acagttttgt gcgcaggctg cagccggaga ttattgtcaa caaccgcgtc ggcaaggggc
     1621 gcaacggcat gcaggggatg agcctcgaca aaagctcggc cggggatttc ggcacccccg
     1681 aacaggagat tctcgcccag ggcggcgcag ccatggactg ggaagcctgc atgactacca
     1741 atgacagctg gggatttaaa aagtcggaca acaactggaa aagccccgaa gccctcatcc
     1801 ataacctgat cgatatcgcc gccaagggcg gcaactatct gctcaacctc ggtccggacg
     1861 ccgaagggct tatccccgaa gccggccagg agcgcttccg cgagatcggg aaatggctca
     1921 aggtcaacgg cgcggccatt tatggcacca gacccctgcc cgaataccgg gagggcgaga
     1981 atatccgtta cacccgcagt ctggacggca agaccatcta cgccactgtg ctggaatggc
     2041 cgggcaagag cctgcgtctg aagtacatcg aaccgaaaaa gaaatccaaa atctatctgc
     2101 tcggccgcaa gagcgccctc acctggaaag ccaacagcag gggggaggtg gtcatcaccc
     2161 tgccggccct gctgcagcgc gccgccaacc gcccctgccg ccacgcctgg gttttcaaaa
     2221 tcaccggccg ccagctgcgc gtggccgctg ctccgctgat ctcgaccgcc agcaagacgg
     2281 gcgttgtccg gagtcttttt accgacagcg aacagatcac tatcagcgcc ccctccgatg
     2341 gcgcggaggt gcattacacc ctggacggcc gcaccccaag ccagaattcc ccggtctaca
     2401 ccgcgccgat caccctcacc gaggccgccg agatccaggc tgtggcgctc gagaagggca
     2461 aggtgatcag tccggtcacc accgcccagt tctccagaac cacccgcatc aagcgcctga
     2521 ccctcgagaa gagcccttcg gtcaagtacc ccggctgggg cgagctgggg ctggtcgacg
     2581 gccgccaggg gggcacggat taccatgaga gcgactggat cggctttgag ggcgagagcc
     2641 tcgatgccac catcgatctg ggggaacaga agagcatctc ctccatccgt ctgagctgcc
     2701 tggaggacca gaactcctgg atctttttgc cttcttccat caaactcctg acctcgacgg
     2761 atggggcgac cttcgtccag accgctgaat ggaagggaga gacccgcccg gccggaacgg
     2821 cagcgacccg ggagactgcc ctgatcatca tgcccaagag ctgccgctat ctgcgcatcc
     2881 tggccagcaa cgtcggcgtc tgcccctcat ggcacaaggg tgccggcggc aaagcctggc
     2941 tttttatcga cgaaatcatc atcaactgag gaggttttat ggctgctgga gaccatccgg
     3001 acaaagacac ccagggggtg gaccggcgat catttttcaa gaccggcgtg ctggcggggc
     3061 ttggcgccct gaccgccgca tcgaccctga accgctgcga tgccactgca agcggccgcg
     3121 gaccggcgcc tgcccagctc ttctccgtgg cgcccctgaa aaaggtgcgc atcggcatgg
     3181 tcggggtcgg actgcagggc acctcgcatg tgcgcaattt tctcggcatc cccgaggccc
     3241 aaatcaccgc ggtctgcgac atccggcccg agcgggtggc ggcggcccag aaactgatca
     3301 ctgcggcggg ccatccggaa ccgcgcggct acagccgcgg cagcaccgat ttcctccgtc
     3361 tctgcgatga agaggaactg gacctggtct tcacggcgac cccctgggag tggcatgttc
     3421 cggtctgcgt cgccgcgatg aagagcggca agcatgccgc gaccgaggtt cccgcggcgg
     3481 tgaccgtgga tgagtgctgg cagctggtcg aaaccgcgga gaagcaccac aaacactgcg
     3541 tcatgatgga gaactgctgc tacgaccgcg aggagctgct catcctcaac ctggtgcgca
     3601 agggcatgct cggcgagatc atcaacgccg aggcgggcta tctccacgat ctgcgcaacg
     3661 tcaagtttaa caacgaaaat gaggcggtct ggcgtctgcc ccactccatc aagcgcgacg
     3721 gcaatctcta tcccacccac ggcctcggcc cggtcgccca gtgcatgaat atcaaccggg
     3781 gcgaccagtt cgactatctg gtctcgatga gcagcgtctc gcggggattg aatctctacg
     3841 cctcccggct ctacggcgct gaggatccgc gcgccaccca gcgctacgcc ctcggtgatg
     3901 tcaataccac cctcatccgc acggtgctgg gcaagaccat caccctgatc cacgatacct
     3961 ccacgccgcg cccctatacg cgcatcaacc gggtgcaggg cacccgcggt ctggccgaga
     4021 aatggccgca ccgggtctat atcgagggca agagcgagcc ccatcggtgg gatgacctgg
     4081 cggactatta ccaggagcac gaccatcccc tctggcggga actccagcag agggcggccg
     4141 gatcgggcca cggcggtatg gattttatcg agtgctaccg tctgatccac tgcctgctca
     4201 acggccagcc catggatatg gatgtctacg atgccgcctc ctggagctgc atctcggagc
     4261 tgagcgagcg ctccgtggcg cgccggggcc ggcccatgga tgtgcccgat tttacccgcg
     4321 gccgctggaa aaactggcct caactcggga tcatctccgc ctgatgactc tgcattgcga
     4381 caggggcctc tccccctccc gttccggggc gggagcaggc cccctccact cccttgcgtt
     4441 cccgcccgca accatgggaa ccccgaaccg ccaatatgga gattcacatc atgaccagct
     4501 gtgctcgaat cctgatcccg gctctcctgg ccgccctgtg cggctgcgcc ggcgccccta
     4561 ggatgcaggc gcccagcgca cagaccggcc ccgccctgat tccgctgccg gttaaaatga
     4621 cggccaggcc gggccatttc cggctcgatg gctcgaccgt cactgcctcc tccggcgagg
     4681 cggaggaggt ggcggaatat gtggcgcagc tcatccgcag gggcaccggc tggcagctgg
     4741 cgcgggcggc gggcgtcccg cccgacagca atgccattgt cctggagctg accggttccc
     4801 ccgccgcttc ggacgcagag ggctataccc tggagatcac cccgcagcgc atcctcataa
     4861 aagccgcccg gcccgccgga ctcttttacg gtgtccagac cctgcgccaa ctctggcccg
     4921 ccgcctggga ggggccggca ccggccgttc aggctgaacc ggcgcgcatc ccttgcctcg
     4981 tcatcaatga ccagccgcgc tacagctgga gggggggaca tctggatgtc tgccgccact
     5041 ttttccccat cgaattcatc taccgctaca tcgactatct ggcgatgaac aagatgaaca
     5101 ccttccactg gcatctcacc gacgatcagg ggtggcggat cgagatcaaa aaataccccc
     5161 tgctgaccga ggtggggggg tggcgcgtcg accgctccgg tgaccactgg aacaagcggg
     5221 ctccgcagca gccgggcgaa aaggccacct acggcggcta ttacagccag gaggagatcc
     5281 gcgccgttgt ggagtatgcc agcagccgct ttatcaccat cgtccccgag atcgagatgc
     5341 ccgcccatgc cggggccgcc ctggccgcct atccccgctt ctcctgcacc ggtggtccct
     5401 ttaccgtacc ccccggcggc gtctggccga tcgtcaacct ttactgcgcc ggcgatgaca
     5461 ccaccttcag ctttcttcag gatatcctcg acgaggtgat cgggcttttc cccggcgatt
     5521 atatccacat cggcggcgac gaagccgaca aaagcgaatg gaaaaaatgc cccaaatgcc
     5581 aggcgcgcat caggaccgaa aatctcgggg atgaaaccgg actgcagagc tatatgatca
     5641 gacggatgga gggctacctc cacagccggg gcaagcgcat catcggctgg gacgagatcc
     5701 tcgagggtgg attggcgccg cgggccgccg tcatgtcctg gcgtggcatg cagggcggga
     5761 tcgacgccgc ccgcgccgac catgatgtgg tcatgacccc gaccgattac tgctacttcg
     5821 actattatca ggggccggcg gattttgagc cactctccat cggcggctat attcccatcg
     5881 agaccgtcta tgccttcgag cccaccccag ccagcctcga tgaaaagcag gcgaagcata
     5941 tcctcggctg ccaggccaac ctctggaccg aattcatcgc cacccctgaa catgccgaat
     6001 acatgctttt tccccgcctg ctcgccatgg ccgaggtggc ctggtcaccc aaatcctccc
     6061 gcggctggga ccactttctc gaccgcctca ccgccttcct gcctcgcctg gaggcgatgg
     6121 gcgtgaacta ttcccgctcg ctttacagcg tcaaacccgt cctcgaacgg agcagcgacg
     6181 gccgcagcgc actggtcgcc ctcaagagcc ctgccccatt cgccgtcatc cactacactc
     6241 tggatggagc cgagcccacg gccgcctcgc cccgctacga gagccccatc accctgcagg
     6301 gcagcagcac catcaaggcc ggcctcttcc tcgacggcgc cctccagggc aggattgtcg
     6361 ccgtgccttt cctggtccac aaggcctccg gccaaaaggt cacttacacc cacccctgga
     6421 gcgatcgcta ccaagccgca ggccccaccg cactggtcga cggcctcacc ggcagcaaga
     6481 attacaacga cggctcctgg cagggattcg aacagcacga tctcgaagtg gtcatcgatc
     6541 tcggcaggga aatggagatc cggcgcgtct cgagcagcta cctggaggac ttcggctcct
     6601 ggatcttcgc cccccgggag gtcagctatg ccttttcgga caaggggaag gagtttcaaa
     6661 ccctcgcggt gctgccgggc ggcgggatgc aggatggaaa cgaaatcacc atccaccatt
     6721 tcggccgcga tttcagcaat ctgcgggcgc gctatctcaa ggtgactgct aaaaatatcg
     6781 gggtttgtcc ccctgatcac gccggagcag gcgggaaagc ctggctcttt gtcgatgaaa
     6841 ttattgtgga gtgatcttca tgcaccgtac cggtttctgt cttcttctga tgctgctcgc
     6901 cggcggggcc gccgccggag ctgatctcat ccccctgcct gccagtgtca cgaccggaga
     6961 aggaagctgc accctcaccg cttccacgct tctttactac gatcccgccg tgcctgaact
     7021 gcagggctat gcggagcgtt tcgccgcccg gcttcgcctt gccaccggcc tccccctgcc
     7081 cctgcagccc ctgcccgcgg acaccagcgc cgccgacgcc ctctatttca ccgcccacgg
     7141 cgcccgtgcg gagtggggcc gcgagggtta cagcctcgag gtgaccacgg cgggggggcg
     7201 catccgcgcc aacgccgccc ccggcttttt ctacggcatg cagaccctgc tgcagctgct
     7261 gcccccggca gtgggatcgc cgacgccggt ccccggcacg gcatggcgga tggagtccgt
     7321 gaccatcctc gaccagcccc gtttcgcctg gcgcggcgtg cacctcgatg tcggccgcca
     7381 cttttttccg gtcgagttcg tcaagaagta cgtcgatatg atcgccctct acaaaatgaa
     7441 caccttccac tggcacctta cggaagacca gggctggcgc atcgagatca aaaagtatcc
     7501 ccggctgatg gaggtcggtg cctggcgcaa cgacggcatc agcgggccct atggcggctt
     7561 ttacacccag gaggaggtcc gtgaaatcgt ggcctatgca gccgagcggg cgatcaccgt
     7621 cgtgcccgaa atcgagatgc ccggccactc tctggccgcc ctcgcctcct acccccgcct
     7681 gggctgcacc ggcggaccct accgtgtgca gttcagctgg ggcgtcttcg atgacgtctt
     7741 ctgtgcgggg cgggattcca cttttacctt tttggaagat gtcctgaccg aggtgatggc
     7801 gcttttcccc ggcgaatata ttcatatcgg cggcgatgaa tgccccaaaa cgcgctggag
     7861 gagctgcccc gactgtcagg cccgcatcaa ggcggagggg ttggcgaacg agagcgagct
     7921 gcagagctgg ttcgtccgcc gcatcgagcg ctttctcagc gccaacggcc gccgcctcat
     7981 cggctgggat gagatcctcg aggggggcat cgcccccaac gccacggtga tgtcctggcg
     8041 cggctttgac ggcggggtgg aggccgcccg cagcggccac gatgtggtca tggccccccg
     8101 ggaatactgc tacctcaatt tctaccaggg cgtgaagagc ggggaacccg cgccgtacgg
     8161 cgattacctg cccatcgaaa aggtctacgc cttcgagccc gtacccgatg tgctcacctc
     8221 cgctgaggcg cagcatgtca tcggtgcgca ggcctgcctt tggaccgagt tcatcactga
     8281 atcccgcaaa gccgaataca tgctctttcc gcgcctctgc gccctcagtg aggtggtctg
     8341 gagcgagccc tcccggcgcg attacgccga tttctccctc agtctgcaga gccattttga
     8401 gcgcctcgag gccgccgata tcaatgttcg catccccccg tccgcctatt tccccggcac
     8461 gggcggcgac gccggtttcg agttccggga tgccggtcct ctctcaggcc tcctccccgg
     8521 cggaggcgcg cgcatcggct cggatgccct gctctgggag atgacctccg acagcctgcc
     8581 gagcggggcg gcgctcacct tcgctgagcc gcaggacatg cgcctgcgca tcccctatta
     8641 cagcctgagc ttcgcctacc ggaccaatct ggcgccggat acactcttcg tcggtttcag
     8701 ggatgaggcc ggacggggct tttatctcca gctcctcaag cgccatatcg ccgcgaccaa
     8761 cacctgggta tccgtgaccc gcgccctctc cggcttcaaa tcggatcctg gattcgacgc
     8821 cagccgggtg gtccggttcg gcttctggac caacagctcc cgcagcggcg cccgcttcca
     8881 gttcgccgat gtctatctcg gcgtgcctca gctctggccg gtggcccact ccccgatcct
     8941 ctttttcaat ggcgagaatt tcaatcccct cttccggatc aagggctttg aaaacgcggc
     9001 gggctcgggt gtagccggtg ctgcgggcat gcgccccggc agcgctgcct tgcgctggct
     9061 gcccccggct gacggaagcc ccagctcggt gcgctggcgc ttcaacgaga ccattgattt
     9121 gagcggagtg ctgggtgcgg acaccctcaa attcgccctc aaggccccgg ccggctgcga
     9181 cagcctgcag atcgccttca ccgatgccgg tcagagagcg tgcagcctct ggctgacacg
     9241 cacagagggg ttctttgacg ggcagtggcg gactctgcag ctcccgctga cccgttttac
     9301 agctgacccg gatttttcag cgcaagcagt cgatctgttc tcgtttttca tccgcagcca
     9361 tcagcccggc gaagaacttt tgctctcgga tatctggatc ggccagccgc cggcctccgc
     9421 catcagcggg atcgaggagc gggccgtcac gcccgcagga tacagcctcg agcagaatta
     9481 ccccaatccc ttcaaccccc tcaccaccat cgcctatcac ctcgccgagc gcggccgggt
     9541 gaggctgacc atccacaatc tgctcggcca gcagatcacc accctgatcg acggagtgga
     9601 ggaggggggg agccaccagc tctactggga tgccagccgt tttgcctcgg ggctctatta
     9661 ctgccgtctc gaggtgaacg gttttgtccg gacccgcaag atggttttgg tgaaataagg
     9721 gggcaaggga tgtccggact ctccatcgcc atcatcgcgc gcaacgaaga ggccaatatc
     9781 cgcgcctgcc tggagagcgc ggcgtgggcg gacgagatcg tcctcgtcga cgcctgcagc
     9841 gaggatgcga ccgtgacgat cgcgcgggaa ttcaccgacc gcatctttgt gcgcaaatgg
     9901 gagggctatg ccgcccagaa ggaattctcc ctgcagcagt gcacctcgcc ctggatcttc
     9961 agcctcgacg ccgacgaacg catccgcccg gaactcaggg aggcgatgca agcggtcatc
    10021 gcggatcccc atgccctggc gggctatcgc gtcggccggc gctcctggtt cctcggccgc
    10081 tggatccgcc acggcggctg gtatcccggc tatcagctcc gcctcttccg ccgggaggcg
    10141 gtgcggatga tccgccgccg cgtccatgag ggctttgagg ttgagggtcc gatcggcacc
    10201 ctgaccggtg atctcgacca tttctcccat cccacgatcg ctggcagcct cgacaagatg
    10261 aaccgctact ccaccctcga ggccttcgac cgcctcgagc gcaagcgggt gcacgccatc
    10321 gactttttca cccatccctt cgccgccttt ttgcgcaagt acatcgccca gggcggcttc
    10381 cgcgacggcg tcaacggact ggttctcgcc tgggtcaccg ccgttgtcaa gctggccctc
    10441 tacatgaaaa tccgggagct gcagcagcgg ccggcggggg agaccgcaga ggctgcgggc
    10501 ggagagctga tgcgcctctt cgatgaatat caggcggtac tggagcagta tcagcccgcc
    10561 gccgggaagc aggggcgcaa gcccggtctg aaggcctttg tcttccagcc cgccgcggcc
    10621 ttttggcggc attacgtccg ccgcggccgt cctgggggcg tgccaggact gatgcaggcc
    10681 ctgctcgccg ccgtctatgc caccgcccgc gaactcgcca attgggaagc gggtctctcg
    10741 aatacctcca gctgaacatc atctcaaacc tggtgcctca tgaaaaagct cacactattt
    10801 ttgttgttcc tccccgtcat cgccttgacc cagcccccgc gccaaaagct gctttttgat
    10861 ttcggctgga aattcgctct cggccacgcc gccgatccgc agcgggattt cggctacggc
    10921 atcggcgccg ctttcgccaa ggccggcgag gcccagggcg ccgcccaccc caatttcagc
    10981 gacagcctct ggcgcagcgt cgacctcccc cacgactggg cggtcgagct gcctttcgtc
    11041 aatcatgaag atccggaggt caagtcgcac ggctacaagc ccgtcggccg gctctttccc
    11101 gaaagctccg tcggctggta ccgccgcgcc ttcgccatcc ccgcggccga ccgcggccgg
    11161 cgcatccgcg tcgacttcga cggggtcttc cgggacgctg ccgtctggtg caacggccac
    11221 tacctcggcc gccacgccgg cggctatacc cccttcagtt ttgatctcac cgactacctc
    11281 cgctacggcc agcgcaacac cctggtcgtg cgcgtcgatg ccagccacta cgagggctgg
    11341 ttctatgagg gggccggcat ctaccgccac gtctggctgc ataaatacga gcccctccat
    11401 ctggcggaat acggggtcta tgtacacagc cagatcagcg gcgccgctgc ggaagtgacg
    11461 atcgaaacca tcgtggaaaa tctctcccct gcggcagcgg aaggccgggt ttgcgtcgat
    11521 ctgctcgacc gcgatcgccg cctcgccggc cgctcggtgc tgccctttgc tgccctgcag
    11581 ccgctggagg cgacgaccat ccgccatgcg atcaccgtgg cggacccccg gctttggtcc
    11641 ctggaggatc cccatctcta tacggttcac accgtgatcg agcaggaggg ccggaccatc
    11701 gacagccttg ataccccggt cggcctgcgc agcctccggt tcgacccgga tcgggggttt
    11761 ttcctcaacg gcgcgcctgt caagattcag ggggtctgct gccaccagga ccatggcggg
    11821 gtcggatcgg ccctgcccga cgccctccag gagtaccgca tccggctcct caaggcgatg
    11881 ggctgcaacg cctaccgcac cagccacaat ccccccacgc ccgagctgct cgatgcctgc
    11941 gaccgcctcg gcatgctggt tatggatgag caccgcctga tggggagttc gcccgaaatt
    12001 ctcgaggaac tcgaaaccct ggtcaagcgc gaccgcaacc atccttcggt catcctctgg
    12061 tcgctcggca acgaggagta cgt
//
LOCUS       DAOPWU010000075        12031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5412_length_12031_cov_4.228958, whole genome shotgun sequence.
ACCESSION   DAOPWU010000075 DAOPWU010000000
VERSION     DAOPWU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5412_length_12031_cov_4.228958"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..2067)
                     /locus_tag="PKM23_05695"
     CDS             complement(58..2067)
                     /locus_tag="PKM23_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91003.1"
                     /translation="MQKKNISFSRCAIGLFLLTGLIPAAGQEQNYTIELRRGRLWHSF
                     YFSQECEPMADWTRKSAGLDWPGFSSEEIKQNIGGSNSYLVAGGFFITALNDSGEVWG
                     WDNFSNHGTDVGWKGDAFRYLVKRHQLRWKQGENYWLAADPREAEVVLETEFEMNGAW
                     YQPWDNQNLPIQIKRRVRQWSGSRADEDYLIIEYVIANTQRRKPLRGVWLLFSYALSP
                     NHRGWNLTFPNFPPGARNARSRWNPEEKLLTCRAGDLAATPGFDESYDYFEHYIYDPV
                     ADRNRIEPEFVAPGVMGLKFLALPPDSNGVEGINDFVWSAAPATNDHAAPFLGVTGLD
                     AKYAAMADPMLLSEAFRDPADPRMGSSRLYANYSLGPFNIPARDSIRVVMAEFVGGMP
                     YERALQPGVTRAMVEAAADSAAAYLNARIAFNYQHKYTVPMPPPAPPFTLSSYREPAR
                     VGNVISFDARSESVPDPHQKVVDLAGYRIYRSGRYPFGPWQQIADIQAGDSRYYNPAS
                     GRYTFIDEGVPLGYGFYYSVTAYDTGHPAWAVDAGVAVPPLESSIFANRTTTPFYTTL
                     TPTEADLSSVTVVPNPFYRSSGFELAGDAKLIQFVNLSTACTIEIYTVRGDLVKTIRH
                     SDPQSGVATWNQISDYGQYVKSGMYFYRVHNDRGEVKSGKFAIVN"
     gene            complement(2078..4972)
                     /locus_tag="PKM23_05700"
     CDS             complement(2078..4972)
                     /locus_tag="PKM23_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNY91004.1"
                     /translation="MKKITAFLLFLLLSAAPARLHAQTTGKIAGQVRDAATREPLAGA
                     NVMVVGMAIGAVTDMEGRYAILNVPPGMHQLRFVYIGYQPLTVAQVGVRIDRTTTIDA
                     QLKTTTVDLGESVTVTATRPIVEADKTSSSVHYDTDEIDALPVEGIRNILELSAGINK
                     NADGTYSIRGGGSYEINYQINGMKSLTTNTGVPAYGTGSKSDNSWKYDINPLAIAQME
                     VISGGFNAEYGNAQSGVVKVVTKEGGPRFNGAFIMEYRPEGQYHWGDYLYSQQQYEWR
                     KWGLVENWLKEPQFRDSLGVVNEAEARRNHQLWVKNHTPSDDNIFGIYDYRQHPYTRY
                     LFSFGGPLGMNAEKMSFFFSGELKNKPTRLPTREKIQRLQNYSLVLAWKPSSRQNFKL
                     TGLYQSYFSGMGSGSDDITWAGLWGSYGAKRKYTLIYDSPREETVFAQNLNYKYLFSA
                     RSYLEANLTHQSETLYALQTPTPATDVDRKLEVESRRLEDPGPWFEKYRAYYTWSSLY
                     NQASVTRFYEGRLNFTSQLTNTNLFKAGIETSLMDQNYNASSSLCVSAYIWRTGFATN
                     YKAKTWYAAGYVQDKLEFAGMVANLGLRLDAYNFGVDVPADRYDLFYPALGSPAVGNP
                     AWEPSRTFTSLSPRLGVSFPIGERTAFRVQYGHFRSMPLVNQALDNQTYNGWGSYGNA
                     NLKPKLSINYEVGVQQNLWDTHQLDIVTYYNDLKDQISTVYVKSTTGSQNKEDDLKGS
                     YISYENRGYGNARGIEITFQNRNSQIWHYRLSYTLSQANYGNYGVYLEREVMTPELEQ
                     KYQYSASDYLAPEDRTHRFNGMVTYFVPAGSGLELFGRRPLANLSISLIYKVTSGLAY
                     YWSPEYQYEYQVESNRRYPMESQTDLRVEKDFTLMDLKLKASLRVLNLLGNKHLTPIY
                     TSDELDRFVLRSVTYMDRDDDPYRDYRLYNYFQTFKNIPRQLFLSLGVEF"
     gene            complement(5029..6693)
                     /locus_tag="PKM23_05705"
     CDS             complement(5029..6693)
                     /locus_tag="PKM23_05705"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNY91005.1"
                     /translation="MKRLASLLLVISLSASAQPLTWSEVIPSGLPQGARLFSGVRIQP
                     ALRAWYIDIDLNVPDLAVRPYLSTTSSRKETVPDFTARTGAFAAVNGGYFGLADGASY
                     SAVVYPDRVLAQNVAVVTRDNKSYPVTRSLFSLNTSRSPAVDWIYHFGGRPADIYRYA
                     QPAPNVMGTPAPLPLAAAGTPYAEILTAIGGGPTLLKNGQVRISYDEEVFWGSGVGAD
                     NRDPRTAVGYTPGHHVLLMVVDGRQAASEGATLAELAAMLLELGCVEAMNLDGGGSTQ
                     MSIGATLVNRPEGQTSGVRAVPTILALVHADSLIAPHKTVYFEKIIDTADPECTLSGA
                     GWFESANAGYYGATRSLLNTPGQGAKFAQFQPRLRGPALYEIQAWWVASSNRCQDTPF
                     IIQHQGRSDTVRVNQTANGSAWVPIGAFSFADDGTEYIRISDAATRGQYIVADALRIL
                     SHDARTGAASNAIVTPRSPLLLHCYPNPFNNRARIRCRLKEDGHIRLALYDPLGREVA
                     VLADELLMAGDHEWPLAAEALASGLYFVTLRDSRGGLAAAKVMLIR"
     gene            complement(7043..7492)
                     /locus_tag="PKM23_05710"
     CDS             complement(7043..7492)
                     /locus_tag="PKM23_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNY91006.1"
                     /translation="MKSFAFDAVIQKHPGLDAAFIEFPYNVEEEFGVKGRVKVVATFD
                     GYEYRGSLVKMGSPCHWLGLTQQVRSAIGKNPGDSVHVVLRKDDVPREVEIPADLRRL
                     LDENPDAKRWFDGLAYTHRKEYVRWITEAKRQETRDKRLFKAIEMMR"
     gene            complement(7559..9547)
                     /locus_tag="PKM23_05715"
     CDS             complement(7559..9547)
                     /locus_tag="PKM23_05715"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNY91007.1"
                     /translation="MLRTIQDLQDFGTRYEYAPQRDQSAEYLLERLRILGYQAESDWY
                     VTGLTDLFDLAHAGSDSIWAVSLRGHILRSTDRGASWQLQLTLSDNEPLYGLDFLSAS
                     EGWACGYRGNLYGTKDGGTTWTKTEIAGAPHLWDIAALPNGVVLACGEKGGLYRKEKE
                     SELWQKIALNNTDAFWNLHVHDDEHVWLCSYYGALFFSSDHGRSWVPRLQNSSYRLNA
                     VSFISATEGWVAGEDGLLLHTGDGGNSWQVLETNLSATDQPRDLIMTDRLHGRLIMVS
                     GQILSTRDGGWRWEEEFSLLNLGWGPFFYRMERTQDGSWLACGSRGVLLIKTEGEEWS
                     SRTEHLPESLIHLSRNIQAARKGVTAPEREVVMVAHYDSYSDDPLNYAPGANDNASGS
                     SAVLEAARLGREYEFGATLKFLLVSGEELGMLGSTHYVIKAREEKREIAAAVNGDMIG
                     YPLTGNPARLIAGSYLTRSRLIDSVLVYNTRYGIGATFDVEVDSTGASDYGPFAAAGY
                     EALNFAEGSAREIWGGLDPYYHKPVDTIDKLSPDLIRKGGQIMLAAAAEVAGVVGPTA
                     VAAPPLSAPEDFRLAQNYPNPFNATTRIDFRLPRPGRVALKIYDARGREVAVLLARQL
                     GAGAHSVHWHASGEPSGTYFCRLVSDDWSGQIRLVLLR"
     gene            9996..10379
                     /locus_tag="PKM23_05720"
     CDS             9996..10379
                     /locus_tag="PKM23_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNY91008.1"
                     /translation="MDKKADPTAAELEILQILWQAGPSTVRFVNEKLQEKRGVGYTTT
                     LKLLQIMAQKKLVLRSSQGRSHLYRANLGEEQVQDRAVSKLISTVFSGSAMNLVMQAL
                     GHHQPDPEEIEQLRRMLDEMERDEK"
     gene            10376..>12031
                     /locus_tag="PKM23_05725"
     CDS             10376..>12031
                     /locus_tag="PKM23_05725"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNY91009.1"
                     /translation="MIDRILFSQPWFYALGLTLLHSLWHGALIAALLWLLLRLCRDFP
                     ARLRYGLTVMAMLLWFAAAALTYSYLAGSPGPPPPAGLMAEAARTTSLSIGALLENRF
                     DPAAAVPFFMLFWLAGLVFMSARTLAGLFVVHSLKHRAIYPLPREWQVRIGAWLKEWQ
                     VNRMMPVYLSARVHTPAVVGFFRQTLLLPLSFLTSLPPEQVQVVLAHEVAHFLRRDAL
                     VNLFQTLMETLFFYHPALWWISARMREEREFCCDDYAIARCGHPMVLARALVDMEKLQ
                     TTPLRPVPVLSAAGRLQQRVTRLFKSKENAMELHEKLLALLMLIGLGLVLVPLQGLSK
                     SANGTAADLRDDQKKKIEVRVVTEGEKEKVFTVISPDSGAKENEKVLVVKGDDGEEHV
                     FKIKSGKTVSVTKDGKEIPLEELKKEMIDDELLDWDADEDEDKDRVITIESCGDDSTR
                     KHITVKVMDADEDSSLWNVTKDHEKIITINSDGDSVKKRILVRVREGEKDFTITHRDS
                     VRKKVRIVHEHDKARKTHQQQETAGKEIRAQLAKDGFSGEKPVE"
BASE COUNT         2452 a         3468 c         3724 g         2387 t
ORIGIN      
        1 gcgtttaaat aaaattggct ctctattttc cacctggatg ggtctcttca ccgcgcctca
       61 gttgacgatg gcgaatttcc cgctcttgac ctccccgcgg tcattgtgca ccctgtagaa
      121 atacatcccg cttttgacat actggccgta atcgctgatc tggttccagg tggcgacgcc
      181 ggactggggg tcgctgtgcc tgatcgtttt gaccagatcc ccgcgtacag tatagatctc
      241 gatggtgcac gcggtggaaa ggttgacgaa ctggataagt ttggcatcgc cggccagctc
      301 gaacccggaa ctgcggtaaa agggattggg cacgaccgtg acgctgctga ggtcggcctc
      361 ggtcggggtc agcgtagtgt aaaaaggcgt cgttgtgcga ttggcgaaaa tcgaactctc
      421 aagcgggggt acggcaacgc cggcatcgac agcccaggcc ggatggccgg tgtcataagc
      481 ggtcaccgaa tagtaaaaac cgtagccgag ggggacacct tcgtcgatga aggtatatcg
      541 tcccgaggcg ggattgtagt agcggctgtc accggcctga atgtcggcga tctgctgcca
      601 ggggccgaag ggatagcgtc cggagcggta gatccggtag ccggcgaggt ccactacctt
      661 ctgatgcgga tccggaaccg actcgctgcg ggcatcgaag ctgatgacat tgcccacccg
      721 cgccggctcg cggtagctgg agagggtgaa ggggggcgcc gggggcggca tcggcacggt
      781 gtatttgtgc tgatagttga aggcgatgcg cgcattgaga taggccgcgg ccgaatcggc
      841 ggcggcctcg accatcgccc gggtcacgcc cggctgcagc gcccgctcat agggcatgcc
      901 gccgacgaac tcggccatca ccacccgaat cgaatcccgt gccgggatgt tgaaagggcc
      961 gagggaatag ttggcgtaga gccggctgct ccccatgcgc gggtcggcgg ggtcgcgaaa
     1021 agcctccgaa agcagcatcg ggtcggccat ggcggcatat ttggcatcca ggccggtcac
     1081 cccgagaaag ggcgcggcat gatcgttggt cgcgggagcg gccgaccaga caaaatcgtt
     1141 gatcccctcg accccgttcg aatcgggcgg cagagcgaga aatttcaggc ccatcacccc
     1201 cggggcgacg aactcgggct cgatgcggtt gcggtcggcc acgggatcat agatgtaatg
     1261 ctcgaaatag tcgtacgact cgtcgaatcc gggtgtggcg gccagatccc ccgcccggca
     1321 agtcagcagc ttctcttccg ggttccagcg cgaacgggca ttgcgggccc ccgggggaaa
     1381 attgggaaag gtcagattcc agccgcgatg gttgggtgaa agggcatagc tgaagagaag
     1441 ccagaccccg cgcaggggct tgcgccgctg ggtgttggcg atgacgtatt cgatgatcag
     1501 ataatcctca tcggcccggg aacccgacca ctgccgcacc cgccgcttga tctggatggg
     1561 cagattctgg ttgtcccagg gctggtacca ggcgccgttc atctcgaact cggtctccag
     1621 caccacctcc gcctcgcggg gatcggccgc cagccaataa ttctcgccct gcttccagcg
     1681 cagctggtgc cgcttgacca gatagcggaa ggcatccccc ttccagccga cgtcggtgcc
     1741 gtggttgctg aaattgtccc agccccagac ctcaccgctg tcgttgagag cggtgatgaa
     1801 aaaacccccg gccaccaggt agctgttgct gccgccgatg ttctgcttga tctcctcgct
     1861 gctgaatccg ggccaatcca gaccggcgct cttgcgggtc cagtcggcca tgggctcgca
     1921 ctcctgcgaa aaatagaacg agtgccacag ccgtccgcgc ctcagctcga tggtgtaatt
     1981 ctgctcctga ccggccgccg ggatcaggcc ggtcaaaaga aaaagcccga tggcacaacg
     2041 gctgaaggaa atattctttt tttgcatgcg cgctttccta aaactccacg cccagggaca
     2101 ggaagagctg tcggggaatg ttcttgaagg tctggaaata gttatagagc cggtaatcgc
     2161 gatagggatc gtcgtcccgg tccatgtagg tgacgctgcg caggacgaaa cggtccagct
     2221 catcgctggt atagatgggg gtaagatgct tgttgccgag caggttgagc acccgcaggc
     2281 tggccttgag cttgagatcc atcagggtga aatccttctc cacgcgcaga tcggtctgcg
     2341 actccatcgg gtagcgccgg ttgctctcca cctgatattc atactgatat tccggcgacc
     2401 agtagtaggc caatccgctg gtgaccttgt agatcagact gatggaaaga ttcgccaggg
     2461 gccgccgtcc gaagagctcc aggccgctgc cggcggggac gaagtaggtc accatgccgt
     2521 tgaagcgatg ggtgcggtcc tccggcgcca gatagtcgct ggcggaatac tgatactttt
     2581 gctcaagttc gggagtcatg acctcacgct cgagatagac cccgtaattg ccatagttgg
     2641 cctggctcag ggtgtaggag agacgatagt gccagatctg gctgttgcgg ttctggaagg
     2701 tgatttcgat gccgcgcgcg ttgccatagc cgcggttctc gtaggaaata tagctgccct
     2761 tgagatcatc ctccttgttc tgactcccgg tggtggactt gacataaacc gtcgagatct
     2821 gatccttgag gtcgttgtag taggtgacga tgtcgagctg gtgggtatcc cagaggttct
     2881 gctgcacccc cacctcataa ttgatgctca gcttgggttt gagattggcg ttgccgtaac
     2941 tgccccagcc gttgtaggtc tggttgtcga gggcctgatt gaccaggggc atggagcgga
     3001 aatgaccgta ctgaacgcga aaagcggtgc gctcgccgat cgggaaggaa acccccagcc
     3061 gcggcgagag cgaggtgaag gtcctggagg gctcccaggc cggattgccc accgccggac
     3121 tgccgagggc gggatagaag aggtcatagc ggtcggccgg cacatcgacg ccgaaattgt
     3181 aggcgtccag gcgcagcccc agattggcga ccatgccggc gaactcgagc ttgtcctgca
     3241 catacccggc tgcgtaccag gtcttggcct tgtagttggt ggcgaaaccg gtgcgccaga
     3301 tataggccga aacgcatagc gaggaggagg cgttgtaatt ctggtccatc agcgaggtct
     3361 cgatgcccgc cttgaagagg ttggtgttgg tcagctgtga agtgaaattg aggcggccct
     3421 cgtaaaagcg ggtgaccgag gcctgattat agaggctcga ccaggtgtaa taggcgcgat
     3481 atttttcaaa ccagggaccg gggtcctcga ggcggcgcga ttcgacttcc agcttgcggt
     3541 cgacgtcggt ggccggcgtg ggcgtctgca gggcatagag ggtctcgctc tgatgggtta
     3601 aattggcctc gagataggag cgggcggaaa agagatattt gtaattgagg ttctgggcga
     3661 agacggtctc ctcgcgaggc gagtcgtaga tcagggtgta tttgcgcttg gcgccgtagc
     3721 tgccccacaa tcccgcccag gtgatgtcat ccgaccctga acccatcccc gagaagtagc
     3781 tctggtagag cccggtaagc ttgaaattct ggcggctgct cggcttccag gcgagcacca
     3841 gggagtagtt ctgcagacgc tggatctttt cccgcgtcgg cagcctcgtc ggtttgttct
     3901 tgagttcgcc ggaaaagaaa aagctcatct tctcggcgtt catacccagc gggccgccga
     3961 aactgaatag atagcgggtg tagggatgct ggcggtaatc atagatgcca aagatgttgt
     4021 catccgaggg ggtatggttc ttcacccaga gctgatgatt ccgccgcgcc tccgcttcat
     4081 tgaccacccc gagcgaatcg cggaactgcg gttccttgag ccagttctcc accaagcccc
     4141 attttcgcca ttcatactgc tgctgcgaat agagataatc cccccaatgg tactggccct
     4201 cgggacggta ttccatgatg aaagccccgt tgaagcgggg gccgccctcc ttggtgacca
     4261 ccttgaccac accggattgg gcattgccat actcggcgtt aaagccgccg gagatcacct
     4321 ccatctgggc gatggccagg ggattgatgt cgtatttcca gctgttgtcc gatttggagc
     4381 cggtgccgta ggcgggcacc ccggtgttgg tcgtcaggct cttcatgccg ttgatctggt
     4441 agttgatctc gtaggagccg ccgccgcgga tgctgtaggt gccgtcggca ttcttgttga
     4501 tccccgcgga gagctcgagg atgttgcgga tgccctccac cggcagggcg tcgatctcgt
     4561 cggtatcata atgaaccgac gaggaggtct tgtcggcctc gacgatgggc cgcgtggcgg
     4621 tgacggtgac cgattccccc agatcgacag tggtggtttt aagctgggca tcgatggtgg
     4681 tggtgcggtc gatgcgcact ccgacctggg cgaccgtcag cggctgatag ccgatgtaga
     4741 cgaagcgcag ctgatgcatg cccggcggta cattgaggat ggcatagcgg ccctccatgt
     4801 ccgtcaccgc gccgatggcc atgcccacca ccatgacgtt ggcgccggcg agaggctcgc
     4861 gggtggcggc atcccgcacc tggccggcga tcttgcccgt cgtctgggcg tgcaggcgcg
     4921 ccggagcggc gctcagaagc agaaagagca gaaaagccgt tatttttttc atagccctgc
     4981 aaccatcatg ggtgaaagtc tcatgcgatg ggacgttgcg cccccgcctc acctgatcag
     5041 catcactttg gccgctgcca aaccgccgcg gctgtcgcgc agcgtgacga aatagaggcc
     5101 gctggccagg gcctccgctg cgagcggcca ttcatgatct cccgccatga ggagctcgtc
     5161 agccagcaca gccacttcgc ggccaagggg atcatacaac gccagccgga tgtgtccgtc
     5221 ttctttcagc cggcagcgga tgcgggcacg gttgttgaag gggttgggat agcagtgcag
     5281 cagtagaggg gaacgcggcg ttacgatggc gttcgaggcc gcgcccgtac gggcatcatg
     5341 ggagagaatt cgaagggcat cggcgacgat gtactggccc ctggtggcgg cgtcggagat
     5401 gcggatatac tccgtaccgt catcagcgaa cgagaaagcg ccgatcggga cccaggcgct
     5461 gccgttggcg gtctggttga cgcgcactgt gtcgctgcgc ccctgatgct ggatgatgaa
     5521 aggggtatcc tgacagcggt tggaggaggc cacccaccag gcctggatct catagagcgc
     5581 cggaccgcgc aggcgcggct ggaactgggc gaatttggca ccctgcccgg gtgtgttgag
     5641 caggctgcgg gtggcgccat aatagcccgc attggccgat tcgaaccacc cggccccgct
     5701 caatgtgcat tccgggtcgg ccgtgtcgat gatcttttca aaataaacgg tcttgtgggg
     5761 cgcgatcagg ctgtcggcat gcaccagggc caggatggtc ggcacagccc gcacgccgct
     5821 cgtctgaccc tccggccggt tgaccagggt ggccccaatg ctcatctgcg tcgagccgcc
     5881 gccgtccaga ttcatcgcct cgacgcagcc cagctccagc agcattgcgg ccagttccgc
     5941 aagtgtagct ccctcgctgg ccgcctggcg gccgtcgacc accatcagca gcacatgatg
     6001 cccgggggta tatcctacag ccgtgcgcgg atcgcggttg tctgcgccca ccccggatcc
     6061 ccaaaagacc tcctcgtcat agctgatgcg cacctggccg tttttcagca gggtgggtcc
     6121 accgccgata gcggtcagaa tctccgcata gggcgttccc gccgcagcga gcggcagcgg
     6181 cgccggcgtg cccatgacgt tgggcgccgg ctgggcataa cggtagatat cggccggccg
     6241 gccgccaaaa tgatagatcc agtccacagc cggcgaacgg ctggtgttga gtgaaaagag
     6301 gctgcgggtg actggatagc ttttattgtc gcgtgtcacc acggcgacat tctgcgccag
     6361 cacccggtcc ggatagacca ccgccgaata ggaggcgcca tcggcgaggc cgaaatagcc
     6421 gccgttgacc gccgcaaagg ctccggtacg ggctgtaaag tcgggaacag tctccttgcg
     6481 ggaacttgtc gtcgagaggt aggggcggac cgccaggtcc gggacgttga gatcgatatc
     6541 gatgtaccag gcgcgcaggg cgggctggat gcgcacgccg gaaaagagac gcgcaccctg
     6601 gggcaggccg gacggaatca cctcgctcca ggtgagtggc tgagcggatg cggaaaggga
     6661 aatgacgagc agaagagacg caagacgttt catgaggtac ctgtcaaccg atcagccacc
     6721 gaaccagcac cccccgcctg cggggattcc tgcaattgga acaacgagtg cgcccgagcc
     6781 ccgttcgagg gcagccggtt atatcgttcc cgcaggaatt gcagacctct cattatagta
     6841 cacttctgta caaatcgcaa gcaaaatctg cagcagcgac cagaccccat cgatcaggtg
     6901 ttgaaaccag tagcgcctgg gtcagctgag cccctggtgg tctgaataat ccaacaagtt
     6961 actaaaatca gcacggccgc ctgcccccca tccattccag catccttccg cgcctgtcca
     7021 gtggtcaggg gtgtttcacc tgctatcgca tcatttcaat cgccttgaac agccgcttgt
     7081 cgcgggtctc ctgccttttg gcctctgtaa tccagcgcac atactctttg cggtgggtat
     7141 aggccaaccc gtcaaaccag cgctttgcgt ccggattttc atccagcagc cgacgcaggt
     7201 cggccgggat ctccacctcg cggggcacgt catcctttct caggacaaca tggacgctgt
     7261 cgccggggtt cttgccgatg gccgaccgca cctgctgggt caatcccagc cagtggcagg
     7321 gcgaacccat tttcaccagc gagccgcgat attcatagcc gtcaaaagtc gccactactt
     7381 tcacccggcc cttgacgccg aattcctctt ccacgttgta cggaaactca atgaaggcgg
     7441 catccagacc gggatgcttc tgaatcacag catcaaatgc aaagctcttc attccacaac
     7501 tccggtaaaa aatacttaaa aattggcagc tccaaaccca gcagttctgg tccatccatc
     7561 acctcagcag caccagccgg atctgccccg accagtcgtc agacaccagc cggcagaaat
     7621 aggttccact gggctccccc gatgcatgcc agtgcacaga gtgggcgccg gccccaagct
     7681 ggcgcgccag cagcaccgcc acttcccggc cccgcgcatc gtagattttc agcgcgaccc
     7741 ggcccggccg cggcagcctg aaatcgatgc gggtggttgc attgaaggga ttgggatagt
     7801 tctgcgccag gcggaaatcc tccggcgcag agagcggcgg cgcggcgacg gcggtcgggc
     7861 cgaccactcc ggcgacttcg gcggcggctg ccagcatgat ctgccctcct ttccggatca
     7921 gatccggtga taatttgtca atcgtatcca ccggcttatg gtagtacgga tccagcccgc
     7981 cccagatttc tcttgcgctg ccttcggcaa aattgagggc ctcatagccg gccgcggcaa
     8041 aaggtccgta atcgctggcg cctgtggaat cgacctccac atcgaaggtt gcgccgatgc
     8101 catagcgggt gttgtagacc agcaccgaat cgatcaggcg gctgcgggtg agatagctgc
     8161 cggcaatgag ccgcgccgga ttgccggtca gggggtagcc gatcatatct ccattgaccg
     8221 ccgccgcgat ctcacgtttt tcctcacgag ccttaattac atagtgggtg ctgccgagca
     8281 tgcccagctc ttcaccggat acgagaagaa acttgagggt ggcgccgaac tcatactccc
     8341 ggccaagccg tgccgcctcc agcaccgcgc tgctcccgga ggcattatcg ttggcgccgg
     8401 gggcgtaatt gaggggatca tcgctgtacg aatcataatg cgccaccatc accacttccc
     8461 gttccggcgc cgtcactcct tttcgggcgg cctgaatatt gcgtgagaga tggatcaggc
     8521 tctccggcag gtgttctgtg cgggagctcc actcttctcc ctcggtcttg atgagcaaaa
     8581 cccctcgcga accacaggcc agccatgatc catcctgcgt cctctccatc cggtagaaga
     8641 agggtcccca ccccaggttg aggagggaga attcttcctc ccatctccaa ccgccgtcac
     8701 gggtggagag gatctgcccg ctgaccatta tgaggcgtcc gtgcaggcga tcggtcatga
     8761 tgaggtcgcg gggctgatcg gtggcggaca aattcgtttc gagcacttgc cacgaattgc
     8821 cgccatcgcc ggtgtggagg agcagtccgt cttcaccggc cacccacccc tccgtggcag
     8881 agataaagga gaccgcattc aaacggtatg aggaattttg caggcgggga acccagctcc
     8941 tgccgtgatc actggagaag aagagggcgc cgtagtagct gcagagccag acatgctcgt
     9001 catcatggac atgcaaattc cagaaggcgt cggtgttgtt cagagcgatt ttctgccaga
     9061 gctcactctc cttctccttg cgatagagtc cgcctttttc accgcaggcg agtacgacgc
     9121 cgttcggcag ggcggcgata tcccaaaggt ggggcgcgcc tgctatttcc gtttttgtcc
     9181 aggtggtgcc gccgtccttc gtcccgtaga ggttaccacg gtagccgcag gcccagccct
     9241 cgctggccga gagaaaatcg aggccataaa gaggctcatt atcagacagg gtgagctgca
     9301 gctgccacga ggcgccgcgg tccgtgctgc gaaggatgtg gccgcgcagg gataccgccc
     9361 agatggagtc ggatccggcg tgagccaggt caaagaggtc cgtcaaaccg gtgacatacc
     9421 agtcggattc ggcctggtac cccagaattc gcagccgctc gagcaggtac tccgcggact
     9481 ggtcccgctg gggggcatat tcatagcggg tgccaaaatc ctgcagatcc tgtatagtcc
     9541 gcagcatcga gtcgctcgac acttcgttca ccatcgtccg gatgaaaggc gacgaatcgg
     9601 gggcaggcgg ccatgctgcc cggagggctg gcctggcagc agccggaagg gctcgccagg
     9661 ttccggaggg acgcggcggc tgggcgaagg cggatgtgcc gatcagaaag ccgaggaagg
     9721 caaggatggt aaatctgggt atgcgataca taaggccccc atcattgaac tggcattata
     9781 accagagacc gggctgatgc ggttgcagac acgctcgtat acatcttcag gatattacaa
     9841 aatgcagaga atagttccaa ataaaaaacc gaatttccac tggcatccgc cgtacccggc
     9901 ttcatgccat actacgaaaa tattcgtaca aaacagagaa aatacgaaac ctttcgtact
     9961 cattggcgta taattttcca aaaggagaga catggatgga taaaaaagca gatccgaccg
    10021 cggctgaact ggagattttg cagatactct ggcaggctgg acccagcacg gtccgctttg
    10081 tcaatgaaaa gctgcaggag aagcggggtg tcggctatac gacgaccctg aagctgctgc
    10141 aaatcatggc gcagaagaaa ctggtgctgc gctccagtca ggggcgcagc cacctctatc
    10201 gcgccaatct cggcgaagag caggtccagg accgtgcggt atccaaatta atatccaccg
    10261 ttttctccgg ttcagccatg aatctggtga tgcaggccct tggtcatcat caacctgacc
    10321 cagaggagat cgaacagctg cgtcgcatgc tggatgaaat ggagcgagac gagaaatgat
    10381 cgaccgtatc ctcttttctc aaccatggtt ttatgccctg ggcctgaccc tgctgcattc
    10441 cctctggcac ggggcgctta tcgctgcctt gttgtggctg ttgctgcggc tgtgccgtga
    10501 ttttccggcg cggctgcgct acggactcac tgtgatggcg atgctgctct ggtttgcggc
    10561 tgcggcactt acctactcat atctggccgg ctcacccggc ccgccaccgc cggccggctt
    10621 gatggcggag gctgcgcgaa caacctcgct ctctattggc gctctgctcg agaaccgctt
    10681 cgatccggct gcagctgtgc ccttcttcat gctcttctgg ctggcgggtc tggttttcat
    10741 gtccgcgcgc actctggcgg gactttttgt tgtccattca ttgaagcatc gggccatcta
    10801 cccgctgccg cgcgagtggc aagtgagaat cggcgcctgg ctgaaggagt ggcaggtgaa
    10861 ccgcatgatg ccggtttatc tgtcggcgcg ggtgcacaca ccggcggtgg ttggcttttt
    10921 caggcagaca ctgctgctgc cgctctcctt tttgacctct ttaccaccgg agcaggtcca
    10981 ggtcgtcctg gcccatgagg tggcccattt cctccgccgg gacgccctgg tcaatctatt
    11041 ccagaccctg atggagacgc tcttttttta tcaccctgcg ctctggtgga tttcggcgcg
    11101 aatgcgagaa gagcgggaat tttgctgtga tgattacgcc atcgcccgct gcggccatcc
    11161 catggtcctg gcgcgcgccc tggtagatat ggaaaaactg caaaccacgc ctctgcggcc
    11221 ggtgccggtg ctctcagcag ccgggcgcct gcagcaaagg gtgacacgtc tttttaaatc
    11281 aaaggagaat gccatggaac tgcatgaaaa attactggcg cttttgatgc tcatcggcct
    11341 tggcctggta ctggttcccc tgcagggttt gagcaaaagt gccaacggga ctgccgcaga
    11401 cctgcgcgat gatcaaaaga agaagatcga ggtgagagtg gtcacggagg gagagaaaga
    11461 gaaggtcttt acagtgatct ctccggacag cggggccaaa gagaacgaaa aggtgctggt
    11521 agtcaagggt gatgacggcg aagagcatgt tttcaagatc aagagcggta agacggtatc
    11581 cgttaccaaa gacggcaaag agatccccct ggaagaactg aagaaagaga tgatcgacga
    11641 tgagctcctg gactgggacg cggatgagga tgaggacaag gacagggtca tcaccattga
    11701 atcgtgcggc gatgacagca cccgtaaaca tatcacggtt aaagtgatgg atgccgatga
    11761 agactccagc ctttggaatg taaccaagga ccatgaaaag atcatcacga tcaattccga
    11821 tggcgacagc gtaaagaaaa ggatactggt cagggtcagg gaaggtgaga aagactttac
    11881 gatcactcac agagacagcg tcaggaaaaa ggttcgcatc gtccatgagc atgacaaggc
    11941 ccggaaaacc caccagcaac aggagacggc cgggaaagag atccgggctc agctggccaa
    12001 agacggtttc tccggtgaaa agccggtaga a
//
LOCUS       DAOPWU010000076        12016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5420_length_12016_cov_5.419530, whole genome shotgun sequence.
ACCESSION   DAOPWU010000076 DAOPWU010000000
VERSION     DAOPWU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12016
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5420_length_12016_cov_5.419530"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKM23_05730"
     CDS             <1..222
                     /locus_tag="PKM23_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M15A"
                     /protein_id="HNY91010.1"
                     /translation="IDADPVDSIMDDLNRDGKRDYADAGVLYQVADLLSGADWFAPYL
                     GGLARYRATRRHGPFVHVDVRGFSVNWGD"
     gene            complement(295..732)
                     /gene="lysM"
                     /locus_tag="PKM23_05735"
     CDS             complement(295..732)
                     /gene="lysM"
                     /locus_tag="PKM23_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008989029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HNY91011.1"
                     /translation="MGLIDFIKNVGANLFGKGEDEVTTITNLLNTELPNQITNLQVKF
                     DDGVVDLYGQAESQAAKEKAVLLAGNIKGVSKVNDVYLTAPPAVEETTYYEVKSGDSL
                     SKIAKAFYGNAMKYPVIFEANREVIKDPNLIYPGQKLRIPKIS"
     gene            complement(888..2132)
                     /locus_tag="PKM23_05740"
     CDS             complement(888..2132)
                     /locus_tag="PKM23_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HNY91012.1"
                     /translation="MMEFNTPQNQMGLIVAILIITMIGVAVGRLPRLRMNRATIALSG
                     ATLLVLLGAMPLSQAYTSLDLDTLLLLLAMMIIIANLQIAGFFRLVGHHVMHWARSPR
                     QLLALIMAASGLLSALFLNDTIVLMFTPLVLEITLALRRNPLPYLIGLAVSANIGSAA
                     TITGNPQNMIIGIASGLSYARFAARLLPVAVVGLLIAWLILLLIYREEFREHSMTRPP
                     LGAVKWQKPLLTKGCIAVLFMLVNLFSGMPIPLSALAAAALLLITRRLKPERVFREMD
                     WSLLIFFCGLFVVTGSIEAAGLSEKIFRWIEPVADRGVASLSITSALLSNLVSNVPAV
                     LLFRPIIPHFARPEQAWLTLAMATTLAGNLTLLGSVANLIVAESAKQRGVLLSFREYL
                     RAGVPITLLTLLWGILWLALTA"
     gene            2279..3262
                     /locus_tag="PKM23_05745"
     CDS             2279..3262
                     /locus_tag="PKM23_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNY91013.1"
                     /translation="MVTRSQKMRLGIFLTLAFVALVALLAAVLAPRYIQKRDTYMIGF
                     SDISLTGLLEGGTVKYHGLNVGFVSDISIDPANIRRVIVEVSLDPATPIKTDTQAEIT
                     FLGITGIKVIELHAGSDTAAFLQPGEYIQTGRSLTDEITGKAEVIADKAERVLNNIAI
                     LTDTANRDKMITLLDNTNKALTELHDILDKNNAPFTRILANGEAITSDLQETATSARY
                     TVSSLRGLAESDSLQEIIGNLAHFSRTLQEADLLKIVQDFNLTLERTNSILKEAGRRY
                     NESQEEITITMKRAQETMDNLNTFSRLISEDPSILLRGGKPKDIPDYQLEK"
     gene            3264..3884
                     /locus_tag="PKM23_05750"
     CDS             3264..3884
                     /locus_tag="PKM23_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNY91014.1"
                     /translation="MKRLFRLSFLLGLLLLLGCGSRATLARKYYLIESDARVDTWLLD
                     VPEPFLVTACIGPVRVADPYQDTRIAQRSESNELVYYYYNFWADKPGEMAANMLLRAF
                     RESGVFREVSRHNRGEADVMITCAIDALERSREAKNDLVRAAGSFRLVYLQSNTTLLT
                     YEFDKQTRLRKDRSMNAFASAVSKLLFSEMEEFVFRVADYYQYPPD"
     gene            3888..4997
                     /locus_tag="PKM23_05755"
     CDS             3888..4997
                     /locus_tag="PKM23_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HNY91015.1"
                     /translation="MLRIVLTGDTLSLAGVLSREEAGRALTDIRGRLAASAPGRMHLD
                     LSGLERIDSAGVAMIDQLRQELQARGLEAELTRVPAQVSEALKLFSAPAAPAPTPQVR
                     ESLFYRVGDAAVKAAHTAWIMLYLIADTLYFAVVGLFNRRGIRKGEFINQSILIGVNA
                     LPIVALISFLIGFILALQSAAQLRQFGAAIYVADLIAIAMTREMGPLITAILFAGRSG
                     SAIAAELATMGVTEETDALRSMGLNPTRYVLVPKFYAITLTMPLLTVLSIIIGIGGAM
                     VIGYSYLGIGPKPFYQEVMTVLFFRDILTGLIKSVVFAWIIALVGAYYGFSARGGSED
                     VGRVTTAAVVASIFWVILADSLLGLVFYFGQGLEY"
     gene            5016..5747
                     /locus_tag="PKM23_05760"
     CDS             5016..5747
                     /locus_tag="PKM23_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY91016.1"
                     /translation="MREIVRVRGLHAGYDEEEILHDISLTALAGDVTVILGASGCGKT
                     TLLKHLIRLYVPSRGSIELFGREITTMDEPEFDELLQQVGVLFQNGALLNSISVAENV
                     AIPLEQHTTLPPELIARLVHTKLRLVEMDQALDKRPAELSGGMRKRAALARAIILDPP
                     LLFCDEPSAGLDPTTSAGLDYLLLKLRDELGMSMVIVTHVVASIKRIADRCIFMDQGE
                     VLFSGTLKEAESSGLEKIERFFREG"
     gene            complement(5961..6722)
                     /locus_tag="PKM23_05765"
     CDS             complement(5961..6722)
                     /locus_tag="PKM23_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91017.1"
                     /translation="MKKFFAIALVAMLCAGPLRGEDKPIYSAVNVEVGSDFVIRDFIV
                     YPYSSSYLMGYSRLHYSGFELIDYVRLDCRLYRKGALAGTAEFYGDYATYGRNGMRPG
                     CENFYASYIDRVEFDSVAFSITFRSGDGLQPHIDKEGLKVTTARAEEWGPGKKISGLL
                     RNVSGSAIAWPSILICLYREGQLVQYCQAFAEAPGHLLLPGQSASFSSYIGSAPYDAI
                     VYLSNTALPEKPVRSLPRVGMQTASNPVLFYMSQI"
     gene            complement(7304..7894)
                     /locus_tag="PKM23_05770"
     CDS             complement(7304..7894)
                     /locus_tag="PKM23_05770"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HNY91018.1"
                     /translation="MSTAPSLLKPADLAARLAGLGEATPPLSAAHGLMTAVIVGPSEL
                     PPERWIPFALSADGRVPESPGREELETLVLSLYGLYNDTLAYIREGQFQPWLGREDDP
                     ETRMHNLGLWCVGFSKGMQFFEEEWFKSSDRDMAELLVPVFYFIQPERFIPLYLEEEN
                     DEEKRFELELLMQSQLPESVHTIYDFWRDRKESSAP"
     gene            complement(7976..8467)
                     /locus_tag="PKM23_05775"
     CDS             complement(7976..8467)
                     /locus_tag="PKM23_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNY91019.1"
                     /translation="MISKKMEAALNSQINKEIYSAYFYLAMAADLREKYLDGFARFFE
                     VQAKEEMEHAMKFYEYVNEQGGRVVLEAIDKPQADYKDVEEIFTLALNHEKFVTKSIN
                     DLMTLAVKESDYASAGMLDWFIKEQIEEEANMDKGLHQVKMSVKHPHALMMLDARYGK
                     RED"
     gene            complement(8564..10156)
                     /locus_tag="PKM23_05780"
     CDS             complement(8564..10156)
                     /locus_tag="PKM23_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine exchanger family transporter"
                     /protein_id="HNY91020.1"
                     /translation="MMDTLLHSSYFILFLIVAIGIIIGHIQVRGISLDVSAVIFVALI
                     FGHYGYRVPADFQNIGLLLFIFTIGISAGPGFFASFQRRGFQLILTTLVIVVTGAAVT
                     IALSWAFDVDFRMASGLFTGALTSTPGLAAAIEAADSPLASIGYGIAYPFGVIGVILF
                     VRLMPRLLRADLKKAAHDYAAEAFVAFPEILNRNFVVENANLDGKAIGELDIRTMTGA
                     TISRVMHGQTAVTPSPRTLLHLGDLVKAVGTEDALEKSRLLIGPETSREIPLSKGYDV
                     QWVLVTNKAVVGQSLAALHMHTNYNANITRIRRADIDIAPRRTSILRFGDKLLVACDS
                     ENMTQVVKLLGNDNKRLSETDFLPIALGIVSGVLLGRVHIPLPGGMDFNLGLTGGVLA
                     AALILSRIGKTGPIIWSLSGTANQVLRQLGLLFFLAAVGTEAGAHISEAVSQYGPSII
                     LIGAAITLISMIIGILVGCRLFRVNLLTLLGTLTGAMTSTPGLGALNPLTDCNAPAVA
                     YATVYPVALVLTIICAQIISIL"
     gene            10322..10834
                     /locus_tag="PKM23_05785"
     CDS             10322..10834
                     /locus_tag="PKM23_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNY91021.1"
                     /translation="MQFSRYLPAFALRQALILTLAALVLGLAANQLNPRGIAIGAAPT
                     GAAAWDDSPTPVYPRPITLDQLQGLMQRRAALLIDARSREEYLDGHLPGALHIPFEGW
                     FEQIDRVRALPHERWLVLYCSGGTCDLADLLAGELIREGFEKVAVYRGGIAEYRLANP
                     LKRGEEAGRE"
     gene            10827..11270
                     /locus_tag="PKM23_05790"
     CDS             10827..11270
                     /locus_tag="PKM23_05790"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HNY91022.1"
                     /translation="MNRIKFPRIAALLRLGLGALFIYASLGKIMDPAGFALSIDNYRM
                     LPEFLVALMAAVLPWLELLCGLALVFNCWTPGASLLIFAMNAIFIIAIASAMARGLDI
                     SCGCFSTSEAGSRAGLAHLVEDLFFLAAAGYLYLSSIASLQPPQR"
     gene            complement(11271..>12016)
                     /locus_tag="PKM23_05795"
     CDS             complement(11271..>12016)
                     /locus_tag="PKM23_05795"
                     /inference="COORDINATES: protein motif:HMM:NF040249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HNY91023.1"
                     /translation="EAGVAAALLAAENYTGPAEVIEGKEGLYHVLHNVQWSTDTLLRG
                     LGSEWLINECGYKPFPTEALTHQPISAALQLREEHHLTDDLISNVLVKTTKRGADILS
                     DPSKYKPTTKETADHSLPYCIAAALVDGHVLPSSFDEEKLRDPRIWSMLGKITVVADP
                     EIDAMFPGVKRAIVSITTVAGQVYTAQVDNAKGSPENPMSDEEVIDKFTANAEGVMGA
                     EQRARLIDATWALGSRPLPINEFMQLLVI"
BASE COUNT         2331 a         3696 c         3710 g         2279 t
ORIGIN      
        1 atcgatgccg atccggtcga ttcgattatg gatgatctga acagagacgg gaaacgcgat
       61 tatgcggacg ccggcgtcct ctaccaggta gccgacctcc tctccggggc cgactggttc
      121 gccccctatc tgggcgggct ggcgcgctat cgcgccaccc ggcgccatgg ccccttcgtc
      181 cacgtcgacg tgcgcggctt cagcgtcaat tggggcgact gatttccgca gggctgaaaa
      241 aaaagcccgt gcatctcaag atgcacgggc ttttttgtat ttatagggca gaaatcagct
      301 gattttggga atgcgcagct tctgtcccgg atagatcaga ttgggatcct tgatcacttc
      361 gcggttggcc tcgaagatga ccggatattt catcgcgttg ccataaaagg ccttggcgat
      421 cttggagagg ctgtcgcccg atttgacttc ataataggtg gtctcttcga cggcgggggg
      481 agccgtcaga tagacatcgt tcaccttgga gacacccttg atattgcctg ccagaagcac
      541 ggctttttcc ttggcggcct ggctttcggc ctggccatag agatcgacca caccatcatc
      601 gaattttacc tgaaggttgg taatctggtt cggcagctcg gtgttgagca gattggtgat
      661 cgtggtgacc tcgtcctcgc ctttgccgaa caggttggcg ccaacattct tgatgaaatc
      721 gataagtccc attgcaaaac ctcctggtta gtgggtcata ggagcaaatg aattaggctg
      781 gttatactaa aataacagtc ccggggccgt ttttcatccc gggggggcgg ttttcggctc
      841 ttattttgcc acaaccgggc agggcgccgg cgccaggcat gcgcatttca ggcggtgagg
      901 gccagccaga gaatgcccca gagcagggtc aggagggtga tcggcacccc ggcgcgcagg
      961 tattcgcgga aggagagcag gaccccgcgc tgctttgccg attcggcgac gatgagattg
     1021 gcgaccgagc cgagcagggt gaggttgccc gccagggtgg tggccatggc cagggtgagc
     1081 caggcctgtt ccggccgggc gaagtggggg atgatgggac ggaagagcag cacggccggg
     1141 acgttggaga ccagattgct gagcagggcg gaggtgatcg agagcgaggc gactccccgg
     1201 tccgccaccg gttcgatcca gcggaagatc ttttcgctca ggccggcggc ttcgattgat
     1261 ccggtcacga caaagaggcc gcagaaaaag atcagcagcg accagtccat ttcgcgaaag
     1321 acgcgctcgg gcttgagacg gcgggtgatg agcagaaggg ccgcggccgc cagggcggag
     1381 agggggatgg gcatgccgga gaagagattg accagcatga agagcacggc aatgcacccc
     1441 ttggtcagca ggggtttctg ccatttcacc gccccgagcg gcggccgcgt catgctgtgc
     1501 tcccggaact cctcccggta aatcagcagc aggatcagcc aggcgatgag caggccgacc
     1561 accgccaccg gcagcagccg ggcggcgaag cgggcgtagc tgagccccga ggcgatgcca
     1621 atgatcatgt tctggggatt gccggtgatc gtcgccgccg agccgatatt ggccgagacc
     1681 gccaggccga tcagataggg gaggggattg cggcgcaggg cgagggtgat ctccagcacc
     1741 aggggggtga acatgagcac gatggtgtcg ttgaggaaga gggcggagag cagccccgag
     1801 gcggccatga tcagagcgag cagctggcgg ggggaacgcg cccagtgcat gacatggtgg
     1861 ccgacgaggc ggaaaaaacc ggcgatctgg agattggcga tgatgatcat catcgccagc
     1921 aggagcagca gggtgtcgag gtcgagcgag gtgtaggcct ggctcagggg catcgccccc
     1981 agcagcacca gcaaggtcgc cccgctcagg gcgatggtgg cgcggttcat gcgcagccgc
     2041 ggcagacggc ccaccgccac cccgatcatg gtgatgatca ggatggcgac gatcagcccc
     2101 atctggtttt gtggtgtatt gaactccatc atccggggaa taatatagcg cttttaaccg
     2161 gctattgcca accttttctc ccggccgccg gccgggccgc ggttttcatt ttagtgttgt
     2221 ttattagtgc aaaacattgt aaaatatcag aggtataagg ttatgcaaag gacgaaatat
     2281 ggtgacacgt tcccaaaaaa tgcgcctggg tatctttctc accctcgcct ttgtggccct
     2341 ggtcgcactg ctggcggctg tgctggcccc gcgctacatc cagaagcggg atacctacat
     2401 gatcggcttc agcgatatct ccctgaccgg cctgctcgag ggggggacgg tcaaatatca
     2461 cggcctcaat gtgggctttg tcagcgatat ctccatcgat cccgctaaca tccgccgcgt
     2521 catcgtcgag gtcagcctcg atcccgccac cccgatcaag accgacaccc aggcggagat
     2581 caccttcctc ggcatcaccg gcatcaaggt gatcgagctc cacgccggca gcgataccgc
     2641 ggcctttctc cagcctggcg agtacatcca gaccggacgc tccctcaccg acgaaatcac
     2701 cggcaaggcg gaggtcatcg ccgacaaggc ggagcgggtg ctcaacaaca tcgccatcct
     2761 caccgatact gccaaccgcg acaagatgat caccctgctc gacaacacca acaaggcgct
     2821 caccgaactc cacgacatcc tcgacaagaa caacgccccc ttcacccgca tcctggccaa
     2881 cggcgaggcg atcaccagcg acctgcagga aaccgccacc tccgcccgct acaccgtcag
     2941 cagtctgcgc ggcctcgccg aatccgattc cctgcaggag atcatcggca acctcgccca
     3001 tttctcccgc accctccagg aggccgatct gctcaagatc gtccaggatt ttaacctgac
     3061 cctcgagcgc accaacagca tcctcaagga ggccggccgg cgctacaacg agagccagga
     3121 ggagatcacc atcaccatga agcgggccca ggagaccatg gacaatctca acaccttctc
     3181 gcgcctcatc agcgaggacc cctccatcct cctgcgcggc ggcaagccca aagacatccc
     3241 cgactatcaa ctggagaagt gacatgaaac ggctcttccg gctctctttc ctgctgggcc
     3301 tgctgctcct cctcggctgc ggcagccgcg ccaccctggc ccggaagtat tacctcatcg
     3361 aaagcgacgc ccgcgtcgac acctggctgc ttgatgtgcc cgagcccttt ctggtcaccg
     3421 cctgcatcgg cccggtgcgg gtcgccgacc cctaccagga tacgcgcatc gcccagcgca
     3481 gcgaaagcaa cgaactggtc tactattact ataatttctg ggccgacaaa cccggcgaga
     3541 tggcggccaa catgctcctg cgggccttca gggagtccgg ggtcttcagg gaggtctcgc
     3601 gccataaccg cggcgaggcc gacgtcatga tcacctgtgc catcgacgcc ctcgagcgct
     3661 cgcgcgaggc gaagaacgat ctcgtccgcg ctgccggcag cttccggttg gtctacctgc
     3721 agagcaacac caccctgctc acctatgaat tcgacaagca gacgcggctg cgcaaggacc
     3781 gcagcatgaa cgccttcgcc tcggccgtca gcaagctcct tttcagcgag atggaagagt
     3841 tcgtcttccg cgtcgccgac tattatcaat atcccccgga ttaggccatg ctcaggattg
     3901 tgctcaccgg cgataccctc agcctggcag gcgtgctgag ccgggaggag gcgggccggg
     3961 ccctgaccga tattcgcggc cgcctcgccg cctccgcgcc cggccggatg catctcgatc
     4021 tctcggggct tgagcgcatc gacagcgccg gggtcgcgat gatcgaccag ctccgccagg
     4081 agctgcaggc gcgcggcctc gaggccgagc tgacccgggt gcccgcgcag gtgagcgagg
     4141 ccctcaagct cttctccgca cctgcagccc ccgcccccac accccaggtc cgggaaagtc
     4201 ttttctaccg cgtcggcgac gccgccgtca aggccgccca caccgcctgg atcatgctct
     4261 atctcatcgc cgacaccctc tacttcgccg tggtcggcct cttcaaccgc cggggcatcc
     4321 gcaaaggcga gttcatcaac cagtccattc tcatcggggt caatgccctg cccatcgtcg
     4381 ccctgatctc cttcctcatc ggcttcatcc tcgccctcca gtccgccgcc cagctgcgcc
     4441 agttcggggc agccatctat gtggccgacc tcatcgccat cgccatgaca cgcgaaatgg
     4501 ggcccctcat cactgccatt ctcttcgccg gccgcagcgg ctcggccatc gccgccgagc
     4561 tggccaccat gggggtcacc gaggaaaccg acgccctgcg ctcgatggga ctcaacccca
     4621 cccgttacgt cctggtgccg aaattctacg ccatcaccct caccatgccc ctgctcaccg
     4681 tcctctcgat catcatcggc atcggcgggg ccatggtcat cggttacagc tatctcggca
     4741 tcggccccaa acccttttat caggaggtga tgaccgtgct ctttttccgg gatatcctca
     4801 ccggcctgat caagagcgtg gtcttcgcct ggattatcgc cctggtcggg gcttactacg
     4861 gcttcagcgc ccggggcggt tccgaggatg tcggccgggt gaccaccgcc gcagtggtgg
     4921 cttcgatctt ctgggtgatc ctcgccgaca gcctgctcgg cctggtcttt tatttcggtc
     4981 agggactgga gtactagatg gagcgggaga gggaaatgcg cgaaatcgtc cgggtgcgcg
     5041 gcctccatgc cggctacgac gaggaggaga tcctccatga tatctccctg accgcccttg
     5101 ccggggacgt gaccgtcatc ctcggggcct cgggctgcgg caagaccacc ctgctcaagc
     5161 acctcatccg tctctatgtg ccctccaggg gctcgatcga actcttcggc cgcgaaatca
     5221 ccaccatgga cgaacccgag ttcgacgagc tgcttcagca ggtcggggtg ctctttcaga
     5281 acggcgccct gctcaactcg atcagcgtcg ccgaaaatgt cgccatcccc ctcgagcagc
     5341 acaccaccct gccgcccgaa ctcatcgccc ggctggtgca caccaagctc aggctggtcg
     5401 agatggatca ggccctggac aaacggccgg ccgagctctc cggcggcatg cgcaaacgcg
     5461 ccgccctggc gcgggcgatc atcctcgatc cccccctgct cttctgcgac gaaccctcgg
     5521 ccggcctcga tcccaccacc agcgccggcc tcgattacct cctcctcaag ctccgcgacg
     5581 aactgggcat gagcatggtc atcgtcaccc atgtcgtggc cagcatcaaa cgcatcgccg
     5641 accgctgcat tttcatggac cagggggagg tgctcttctc cggcaccctc aaggaggccg
     5701 agagctccgg cctcgaaaag atcgagcgct ttttcaggga ggggtgaccg ccccgcctcg
     5761 ccgcttcgcc atcgacatgc cccctaaaaa ataaagccct gatcatctca gggctttttg
     5821 ctgtcgcctc cgcaggcggg ggtctgcgga ccgagggcga cgaaaataaa gggcccggct
     5881 gcatctcccc tcggcatcga aacactgcgg tatggcggga aagagaggcg tctccagctg
     5941 cggcgtgcag ccgggtcatt tcagatctgc gacatgtaaa agagcacggg attgctggcg
     6001 gtctgcatcc ccacccgggg gaggctcctg accggcttct cgggcagggc cgtattcgag
     6061 aggtagacga tggcgtcata gggcgcgctg ccgatgtaac tgctgaacga ggcgctctgc
     6121 cccggcagca ggagatggcc gggggcttcg gcgaaggcct ggcagtactg cacgagctgg
     6181 ccctcccggt agaggcagat caggatcgag ggccaggcga tggctgaacc ggagacgttg
     6241 cggagcaggc ccgaaatctt tttgcccggt ccccactctt cggcgcgcgc agtggtgacc
     6301 ttgagcccct ccttgtcgat gtgcggctga agcccgtcgc cgctgcggaa ggtgatgctg
     6361 aaggcgacgc tgtcgaattc gacccggtcg atgtagctcg cgtaaaagtt ctcgcatccc
     6421 ggcctcatcc cgttgcggcc gtaggtggca tagtcgccgt agaactcggc cgtccccgcc
     6481 agggcaccct tccggtaaag gcggcagtcg aggcggacat agtcgatcag ttcaaagccg
     6541 gagtagtgga ggcgcgaata gcccatcaga taggaggagg agtagggata gacgatgaaa
     6601 tcccggatga caaaatcgct tcccacctcg acgttgacgg cggagtagat tggtttatct
     6661 tcgccccgga gcggcccggc gcagagcatg gcgaccagtg cgatggcaaa aaacttcttc
     6721 atcgtttcct cataactggc gggagagcag cctccccggc aggatgcggc gctgcctggg
     6781 cgggtgcgct ccccgccgat ggtgttgtgg catgaacaga gttgtgccgt gcaggataac
     6841 aggggcgccc gcgcagagag cggagaggtt agtgctgaaa tggcatggcg cggagggaga
     6901 gatccgcgcg gacgcattat tttactaccg agatgctggg aactgtcgga tgctgaaaag
     6961 gtgttctgga gggtaaaaaa aaggcacagc ccaccgtcgt gagtccgtgc ctgtatgact
     7021 aaattccacc catcccattc cccataaagt atattttgct gcccgtcaac cggttgaatt
     7081 tcccctggaa ctattctaat aaaaggcgat aaaaaaagca agtaaaaata aaaatttttg
     7141 tattttcgcc accattgaaa ttacggggct tagcggaaag aagaattttc gcggccggcc
     7201 aaaagagccc tttttttgcc caattttccg gggtgaaaac ggacataata acatattata
     7261 aaacaagaaa ataaatcgtc atactccggt ccaaaaactg gagttagggg gccgaactct
     7321 ccttgcggtc gcgccaaaaa tcataaatgg tatggaccga ttcgggcagc tggctctgca
     7381 tgagcagttc gagttcgaag cgcttctcct cgtcgttctc ttcctccaga tagaggggga
     7441 tgaagcgctc cggctggatg aaataaaaga ccggcaccag cagttcggcc atgtcccggt
     7501 cgctgctctt gaaccactcc tcctcgaaga actgcatgcc cttggaaaag ccgacgcacc
     7561 agaggccgag attgtgcatc cgggtttcgg ggtcatcctc gcggccgagc cagggctgga
     7621 actgcccctc acggatatag gcgagggtat cattgtaaag gccgtagagg gagaggacca
     7681 gggtctcgag ctcctcgcgg ccggggcttt ccggaacccg cccgtcggcc gagagggcga
     7741 aggggatcca gcgctcgggc ggcagctccg acggcccgac gatcacggcg gtcatgagcc
     7801 catgggcggc gctgaggggg ggcgtagcct ccccgagtcc ggcgaggcgc gcagcgagat
     7861 ccgccggctt gagcaggctg ggtgcagtgg acatgaaata ctccttggtt gcggttaaaa
     7921 aaaaaggaat ggagggctga ctccattcct tgctgtgatt tggtgaaatg aacgatcagt
     7981 cttcgcgttt gccgtaacgg gcgtccagca tcatgagggc gtgcgggtgt ttgacactca
     8041 tcttcacctg atgcagtccc ttgtccatgt tggcctcttc ctcgatctgc tccttgatga
     8101 accagtcgag catgccggcg ctggcatagt cgctctcctt gacagccagg gtcatgaggt
     8161 cgttgatcga cttggtgacg aacttttcgt ggttgagggc gagggtaaag atctcctcga
     8221 catccttgta gtcggcctgg ggtttatcga tggcttcgag gacgacgcgg ccgccctgtt
     8281 cgttgacata ttcgtaaaac ttcatggcat gctccatctc ttccttggcc tgaacctcga
     8341 aaaagcgggc gaagccatcc agatattttt cacgcaggtc agcggccatg gcgagataga
     8401 aataggcaga atagatctct ttattgatct gggaattgag ggcagcttcc atttttttcg
     8461 aaatcataaa tcctccttga tgatcattgg ggttttgggt tggtttcttc aaaggttgta
     8521 acaaaaaaag tgcgaaaata gttccggaat cttgccggcg gggtcacaga atcgagatga
     8581 tctgggcgca gataatggtc aaaaccaggg cgacgggata gaccgtggca taggccaccg
     8641 ccggcgcgtt gcagtcggtg aggggattga gggcccccag ccccggtgtg ctcgtcatcg
     8701 ccccggtcag ggtgccgagc agggtgagga gattgacgcg gaagagcctg cagccgacca
     8761 ggatgccgat gatcatcgag atcagggtga tggccgcacc gatgagaatg atcgacgggc
     8821 cgtactggct gaccgcctcg gagatatggg ccccggcctc ggtgccgacg gcggcgagga
     8881 agaagagcag accgagctgg cgcagcacct ggttggcggt gccggagagg ctccagatga
     8941 tcgggccggt cttgccgatg cggctgagaa tgagggccgc ggcgagcact ccgccggtga
     9001 ggccgagatt gaaatccatg ccgccgggca gggggatgtg cacacggccg aggagcacac
     9061 cgctgacgat gccaagggcg atggggagaa aatcggtctc cgaaaggcgc ttgttgtcgt
     9121 tgccgagcag cttgaccacc tgggtcatgt tctcgctgtc gcaggccacc agcagcttgt
     9181 caccgaagcg gagaatgctg gtgcgccggg gggcgatgtc gatgtcggcg cggcggatgc
     9241 gggtgatatt ggcgttatag ttggtgtgca tgtgcagcgc ggccagggac tggccgacca
     9301 ccgccttgtt ggtcaccagc acccactgca cgtcatagcc cttgctgagg gggatctccc
     9361 tgctggtctc ggggccgatg agcaggcggc tcttctcgag ggcgtcctcg gtgcccacgg
     9421 ccttgaccag atcgccgagg tggagcaggg ttcggggcga aggggtgacc gcggtctggc
     9481 cgtgcatcac gcgcgagatc gtcgccccgg tcatggtgcg gatgtccagc tcgccgatgg
     9541 ccttgccgtc cagattggcg ttttcgacga cgaaattgcg gttgaggatc tccggaaaag
     9601 cgacaaaggc ctcggcggcg taatcatgcg ccgccttttt caggtccgcg cgcaggaggc
     9661 ggggcatcag gcgcacaaag aggatcaccc cgatcacccc gaagggatag gcgatgccgt
     9721 agccgatcga ggccagtggc gagtcggccg cctcgatggc cgccgccagg ccgggggtgc
     9781 tggtcagcgc tccggtgaag agcccgcttg ccatgcggaa atcgacgtca aaggcccagg
     9841 agagggcgat cgtgaccgcg gccccggtga cgacgatgac cagggtggtg aggatgagct
     9901 gaaagccgcg gcgctggaag gaggcgaaaa agcccggccc ggcggagatg ccgatggtga
     9961 agatgaagag cagcagcccg atgttctgaa agtcggccgg gacgcgatag ccgtaatggc
    10021 cgaaaatcag ggcgacgaaa atgaccgccg aaacatccag ggagatgccg cgcacctgga
    10081 tatggccgat gatgatgccg atggcgacga tgagaaaaag gatgaaataa ctggagtgca
    10141 gcagggtgtc catgattttc ctctgtcaaa acggctgaaa tgtagccatt ttcaggcaga
    10201 tttcaaaatt attttgcctt acatgcagta aatatttata ttatcactca ccggcgccct
    10261 gcggcatccc gccgcaccgg ccgccgtcca cctttcaatc cggtcaaagg aagaaacccg
    10321 cgtgcagttt tcccgatatc tccccgcctt cgccctgcgt caggccctga tcctgaccct
    10381 ggccgccctc gtcctcggcc tcgccgccaa ccagctcaat ccccgcggca tcgccatcgg
    10441 cgcggcccca accggcgctg cggcctggga tgatagccca acccccgtct atccgcgccc
    10501 gatcaccctc gaccagctgc aggggctcat gcagcgcagg gcggccctgc tcatcgacgc
    10561 ccgcagccgc gaagagtacc tcgacggcca tctgcccggg gccctccaca ttccctttga
    10621 gggctggttc gaacagatcg accgggtgcg ggccctgccg cacgagcgct ggctcgtcct
    10681 ctattgcagc ggcgggacct gcgatctcgc ggatctgctg gccggtgaat tgatcaggga
    10741 gggattcgaa aaagtggcgg tctatcgcgg cggaattgcg gagtacaggc tcgccaaccc
    10801 gctcaagcgc ggagaggagg ccggccgtga ataggataaa attcccccgc atcgcagccc
    10861 tgcttcgcct ggggctcggc gcactcttta tctatgcaag cctcggcaag atcatggacc
    10921 cggccggctt cgccctctcc atcgataatt accgcatgct gcccgaattc ctcgtcgcct
    10981 tgatggcggc ggtgctaccc tggctcgagc tgctctgcgg cctcgccctc gttttcaact
    11041 gctggacccc cggggcgagc ctgctcattt ttgcaatgaa cgcgatcttt atcatcgcca
    11101 tcgcctcggc catggcgcgc gggctcgaca tctcatgcgg ctgtttttcg acctcagagg
    11161 cgggcagccg cgccggcctg gcccacctcg tcgaggatct cttttttctg gcggccgcgg
    11221 gctatctcta cctcagcagc atcgcctcgc tgcagccgcc gcagcgctga tcagatcacc
    11281 agcagctgca tgaattcgtt gatcggcagc ggccggctcc ccagggccca ggtcgcatcg
    11341 atcaggcgcg cccgctgctc cgcccccatc accccctcgg cgttggccgt gaacttgtcg
    11401 atcacctctt cgtcgctcat cggattctcc gggctcccct tggcgttgtc cacctgggcg
    11461 gtgtaaacct gtccggccac cgtggtgatc gagacgatcg cccgcttgac ccccgggaac
    11521 atggcgtcga tctctggatc ggctaccact gtgatcttgc cgagcatgct ccagatgcgc
    11581 ggatccctga gcttctcctc gtcgaaggag gagggcagca cgtgcccgtc caccagggcg
    11641 gcggcgatgc agtagggcag gctgtggtcg gcggtctcct tggtggtcgg cttgtacttg
    11701 ctcggatcgg agaggatatc cgccccgcgc ttggtggttt tgaccaggac gttgctgatg
    11761 agatcgtcgg tgagatggtg ctcctcgcgc agctgcaggg cggcggagat gggctggtgg
    11821 gtgagggcct cggtcgggaa gggcttgtaa ccgcattcgt tgatgagcca ttcgctgccc
    11881 agacccctga gcagggtgtc cgtcgaccac tgcacattgt gtaggacatg atagagcccc
    11941 tccttgccct cgatcacctc ggccggaccg gtatagtttt cggcggcgag cagggcagca
    12001 gccacccccg cctcgg
//
LOCUS       DAOPWU010000077        11910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5488_length_11910_cov_5.683256, whole genome shotgun sequence.
ACCESSION   DAOPWU010000077 DAOPWU010000000
VERSION     DAOPWU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5488_length_11910_cov_5.683256"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="PKM23_05800"
     CDS             <1..1421
                     /locus_tag="PKM23_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNY91024.1"
                     /translation="ESDKLITGAIEGLLAKLDPHSVYIPKKSMEKVTEQFEGSFEGIG
                     IEFIVLNKVLTVVSPIPGGPSEAIGIMPGDQIIQIDGSSSYGITEEQVFKKLRGPKGS
                     KVKVMIARTGLSEPFEVEIVRDKIPINSLYASFMLDDGKTGYIWLARFAKTTSTELET
                     ALKELRSQGMQQLIFDLRSNTGGLLEQAVEVADKFIPSGHKLVYTRGRLSGADDDYFS
                     TSGSDQPMMPMIVMINHGSASASEIVAGAIQDPDRGLVVGQTSFGKGLVQNQIGLKDG
                     SALRLTVARYYTPSGRLIQRPYDDGLADYYAEGYDDEDENAPAAPDSGRTAYTTRAGR
                     KVFGGGGITPDSSLKAERITRFTNALFGSRIFFELGSRYGNAQRGRYASFEDFRSRYR
                     VGDELLPDLKELLKKNKIEFSQEAYDRDETYIKSMVKAEVARSLYDSKHYYQIIRGED
                     PELTAAHALMPQAQRILRQGGW"
     gene            1425..1970
                     /locus_tag="PKM23_05805"
     CDS             1425..1970
                     /locus_tag="PKM23_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91025.1"
                     /translation="MADRPLQPRGGGRPGLLLALAFTLLCAACFNPFAPELERSAGNG
                     LRITGQSTPAEVLDNFVYAYTLKDSLVYDGLLDSSFVFVYFDPNLGSSGLYASWNRDV
                     ELRTTGRLFRSFETLTLIWDSTIYETQDGNNAELAKTLHLHLFGSRGEFNLTGNAIFN
                     MRKSSYDQKWRITRWKDESQM"
     gene            2089..2670
                     /locus_tag="PKM23_05810"
     CDS             2089..2670
                     /locus_tag="PKM23_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019072149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNY91026.1"
                     /translation="MITDEQKKRFEEIAFEYMDSLYSTALRMTRNTAEAEDLVQDTYL
                     RAYRFFGKFEEGTNFKAWIFKILTNTFINKYRKKIRTPQQVQLDKVEFGLESEDEQKA
                     IKEWDGFDESNYEELFDDDIKAALAQLSDEFRMVILLADVEGFAYKEIADIIDRPIGT
                     VMSRLFRGRRMLQKVLEKYARNEGYIRSKKAFN"
     gene            2682..4505
                     /locus_tag="PKM23_05815"
     CDS             2682..4505
                     /locus_tag="PKM23_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNY91027.1"
                     /translation="MNRSFVLFLLLLFGAGGLAAQGFVEPEVLTAEAFISQGQAVPGS
                     GLEAVVVVKIVPGLHINSNTPAEEFLIPTQLQFAPSPSLSFGPALYPRPLLKKFAFSD
                     NELSVFEEEVKIHTRVQVLPQAAPGRLQIEGTLTYQGCNETSCFSPGEKKFILPLEIV
                     PAGTPVAQDTSALFSAGVQDPGRGIRPAAAAGEGGLQLTPDERRAMEILEHGLFYAIA
                     AFLLIGLALNLTPCVYPIIPLTVSYFGGQSGRSRGSSFVNALLYLIGIALSFALLGVL
                     SGLAGKQWGFLFQSPWFVAVVATIILLMAASMFGAFEISVPTGLLTRFGGAREGLLGA
                     LIMGLTVGVIIAPCAAGIIIGLVGLVAKLGLVAKGGLLFFAMGIGLGLPYLVLATFSG
                     LLNRLPQSGMWMLWVKKFFGFLLIGVAIYFITPQLDLIHDKLLFLLGLLGVVGGLLLG
                     FIGHEEQTRGFRFFRRLLGGVLILLGLHWLNEAIHSRPSELPWVQYQGQGIEELTAEG
                     KPIFIDFYADWCAPCKQLDKETFSDSRVQEQFAGFTILKVDCTKPDAKTRAFMGRFAV
                     TGMPTLLFLTPAGEEIADLREVGFVSPEKFLASLQRALQAR"
     gene            4634..5590
                     /locus_tag="PKM23_05820"
     CDS             4634..5590
                     /locus_tag="PKM23_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNY91028.1"
                     /translation="MELKKVKFIHNPKSGLVRSPRVIRRAIEAVLEEAPFVYDFSETE
                     YRGHAHELARRAVEEGYDAVVAIGGDGTANEVGSALLYTDTALGIIPVGSGNGLARGL
                     SIPIAIRRAARLLLTGRIRTIDTGTIEERNFFIVTGLGFDALVGKLFDDRSLRGPLPY
                     FYIGVREFLFFRPETFRLEFDGRTVERSALLVTVANTTQWGLGVIISPNARPDDGLLD
                     VCIIRNITLLRALFHLPKLFTGKIDKVREYERYQTTSLRISRNKPGPFHVDGEPVDAG
                     QSVQVGIKAQALKVIVPSRKTRPLTVRAISQARRLQRALLNP"
     gene            5809..6567
                     /locus_tag="PKM23_05825"
     CDS             5809..6567
                     /locus_tag="PKM23_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91029.1"
                     /translation="MAFFVLSLSSLIFFILISDRQTRNSVLRPTLEALGEQLFAAVGD
                     DVDKEQLRAGYREFIREAEEQQIDSARVEKVAAEILNLSRRDSLISSRDALRILEKAQ
                     MAVVIPAAPPATKQAQVRDPDRERHTWIAGRSLPPSWDKEEMAVRLKAMQEFQRQMDR
                     IARESAQLKTLEERYRFQQTDSGLRVLMDSQVVETLAKRDTLMWRKLRELEKKQWLMW
                     GPPPPPPHQGREDSLRAGRIFFHPQERDITRREP"
     gene            6802..10281
                     /locus_tag="PKM23_05830"
     CDS             6802..10281
                     /locus_tag="PKM23_05830"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNY91030.1"
                     /translation="MRRLNLVQLDLLLTHLFEIDYDPPRYVKKFSTLLGEQLELPQAC
                     LLLAQQPYARRQIYYLQSKGCKPDLAVDTIVQEFERRRKAPLVRKLQPGDAMACGMNG
                     SVQYLMIVPVASGERSDSRLGHLLFWFNSTRRVTDYERIIGRFARLLAESIVRQRLNK
                     VRDQLAAADRFESNQIKKVIQKSLTALQAAMGCDYSVALVLKRAITDLNNDPARAGVQ
                     NWEVVTEYLKGKTRLAKNRSKATGLWIEESEGLLALALAHWREDPKKAKKGLTFHQLE
                     PGSSMYLCVETGEKGAGLRGCEDEMHEKTLHYMPLVHEGNLIGYLKASYRSKTRLTEL
                     DRIILIDFADFLAERIHNSFIYSLTVDQTRFLQKIKSMYEGADRLEVRNFADLGQFLL
                     DLTQLITETTQIDTVAIGYLTKDVDGEKVLRFPHPSGWDEGASQRFRELPVTEGLAGL
                     AVRARRNVFLAPVEEPQNPSDPEPTVYVDENRSRFVDVRKHPSYLNHAGYRKLSSYYI
                     KVREEKVYANYIFVIEFKNIVLGVIDVEVAQNNWDRFMGIGYLHFFRTLASMLALLFF
                     RVRLAEEHTRMVQAVKWLSDAVYMSPTSVVKTILAMLQSIYGVEKAKIIPGDLFKEPI
                     EIDLLEGGVENYAHLFLGYEAEEIRRFTVPGDRIDTDNLFLLEFKNHLQNSNDFLMYI
                     PDIEEHQPRFFDKRVVEKFERFRSRPRFYSYLAILLGDKSRSLGVLELVSQKVNAFDE
                     LLIDQGFVETWTSQISMALRYALLEEKREGSAETVQQAEPAPIILRQGKVVPFIAAAQ
                     IEHGCDLVGVGKAMTELRSQIESAALTSSVPILIHGESGTGKDLVAKAIHRRSGWAQD
                     RFITQDCGAFSRELVEDALFGHVRGAFTSATEERKGLFECAHGGTLFLDEISNMSLEL
                     QAKFLRVLEDARITPLGSSKSVQVQVQILAATNANLLEKIRAREFRQDLYYRLAAITI
                     NTPPLRRLVEEEPTNLLLFISHFAALGLIPASFTGIAKKSLEALMEFDFPGNVRQLRN
                     LLSSAAFSGRDKVVLEIPEEALAPDPEAQLEDDQSHVLEEVEDFIHKYQRIGRFEHTP
                     EQVMAGIRSALRSAGDDLEKISSFIDDFGTIDVPLAGLLAGVGPTWARRLLKERLRLK
                     LHPRTSLYFREIP"
     gene            10491..10838
                     /locus_tag="PKM23_05835"
     CDS             10491..10838
                     /locus_tag="PKM23_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91031.1"
                     /translation="MENELLLSLLVFAGCAALGGFMARVIIRCAVAAIVGLGIYAVLA
                     HAMGGAANSISRVIGVAVGLCSALYSLIEGLVHAPGVIGLVIGVMAGLHFWKVERERD
                     HGIVRYPRYPSPE"
     gene            complement(10861..11202)
                     /locus_tag="PKM23_05840"
     CDS             complement(10861..11202)
                     /locus_tag="PKM23_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91032.1"
                     /translation="MGVGTLLTRVPWKQVITMLPTIVQTARGLLNASRRPSIVPIEVA
                     DTPRGLALRVEELEENERIQAELVKKMAEQQQGLAEGLEFMAARITALLWLAAIALIL
                     GLAAFIIVLLK"
     gene            11434..>11910
                     /gene="ftcD"
                     /locus_tag="PKM23_05845"
     CDS             11434..>11910
                     /gene="ftcD"
                     /locus_tag="PKM23_05845"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNY91033.1"
                     /translation="MKLVECVPNFSEGRDAAKIDKIAEAIRSSGGVKLLDVDPGRDTN
                     RTVMTFVGEPEPVLAAAFVAIARAAELIDMAGHSGAHPRMGATDVCPFIPVRGVTMAE
                     CAELARRLGERVGRELGIPVYLYEEAAARPERRNLADVRAGEYEGLAEKMAHPDWRP"
BASE COUNT         2289 a         3570 c         3539 g         2512 t
ORIGIN      
        1 tggagagcga caagctgatc accggggcga tcgaggggct gctggccaaa ctggatcccc
       61 attcggttta tattccgaag aagagcatgg agaaggtgac cgagcagttt gagggctctt
      121 ttgagggcat tggcatcgag ttcatcgtgc tcaacaaggt cctcactgtg gtctcgccga
      181 ttcccggcgg gccctcggag gcgatcggga tcatgccggg ggatcagatc atccagatcg
      241 acggttcctc ctcctacggc atcacggagg agcaggtttt taaaaaactg cgcggcccca
      301 aggggagcaa ggtcaaggtc atgatcgccc gcaccggcct ctccgagccc ttcgaggtgg
      361 agatcgtccg cgacaagatc cccatcaaca gcctctacgc ctcgttcatg ctcgatgacg
      421 gcaagaccgg ctacatctgg ctggcgcgtt tcgccaagac cacctccacc gaactcgaga
      481 ccgctctgaa ggaactgcgc agccagggca tgcagcagct catttttgac ctccgttcca
      541 atacgggcgg gctgctcgag caggcggtcg aggtggcgga caagtttatc cccagcggtc
      601 acaagctggt ctacacccgt ggccggctca gcggcgcaga cgacgattat ttctccacca
      661 gcggcagcga tcagccgatg atgccgatga tcgtgatgat caatcatggc tcggccagcg
      721 cttccgagat cgtcgccggc gccatccagg atcccgaccg cggcctggtg gtcggacaga
      781 ccagcttcgg caaggggctg gtgcagaacc agatcggcct caaggatggt tcagccctgc
      841 gcctgacggt tgcccgctac tataccccga gcggccgcct catccagcgg ccctacgatg
      901 acggcctggc tgattattat gccgagggtt atgacgacga ggacgaaaac gccccggccg
      961 caccggattc cgggcgcacc gcctatacca cccgcgccgg tcgcaaggtt ttcggcgggg
     1021 gcggcatcac tccggacagc agcctcaagg cagagcgcat cacccgcttc accaacgccc
     1081 tcttcggcag ccgcatcttc tttgaactgg gctctcgcta cggcaatgcc cagcgcggcc
     1141 gttatgccag ctttgaggat tttcgttcgc gctatcgcgt gggcgatgaa ctcctgccgg
     1201 acctcaagga actgctcaag aagaacaaga tcgagttcag ccaggaggcc tatgatcggg
     1261 acgagaccta catcaagagc atggtcaagg ccgaggtggc ccgttccctc tacgacagca
     1321 agcactatta ccagatcatt cgcggtgaag atcccgaatt gaccgctgca catgccctga
     1381 tgccccaggc gcagcgtata ctgcggcagg gcgggtggtg aacaatggct gacaggccgc
     1441 tgcagccccg gggagggggc cggcccggtc tcctcctggc cctggccttc accctgctgt
     1501 gtgccgcctg tttcaatccc tttgcgcccg aactggagcg cagcgccggc aacggcctgc
     1561 gcatcaccgg gcagtcgacc ccggcagagg tgctggacaa tttcgtctat gcctacaccc
     1621 tcaaggattc actggtctat gacggcctgc tcgacagctc cttcgtcttt gtttacttcg
     1681 atcccaatct cggctcctcc ggcctctatg cctcctggaa ccgggatgtg gagctcagaa
     1741 ccacgggccg ccttttccgc agtttcgaaa ccctgaccct gatctgggat tcgaccatat
     1801 acgaaaccca ggacggcaac aatgcggagc tggccaaaac ccttcatctc catcttttcg
     1861 gcagcagggg ggaattcaat ctcaccggca atgccatctt caacatgcgc aaaagcagct
     1921 acgatcagaa gtggcgcatc acgcgctgga aggacgaatc ccagatgtag ggccgggcgc
     1981 gagcgaaaaa ctcattgact tattagtact atatttagta ttttttattg attgggcatg
     2041 ggggcaactg gtgacagatg ggtcaatcct gactattatg ggaccgctgt gatcacagac
     2101 gagcaaaaaa agcgattcga agagatcgcc ttcgagtata tggattccct ctacagtacg
     2161 gccctgcgca tgacgcgcaa caccgcagag gcggaggacc tcgtacagga cacctacctg
     2221 cgcgcctacc gttttttcgg caagttcgag gaggggacca acttcaaggc ctggattttc
     2281 aagatactca ccaacacctt catcaacaaa tatcgcaaga aaatccgcac cccccagcag
     2341 gtgcagctgg acaaggtcga attcggcctc gagagtgagg atgagcagaa ggccatcaag
     2401 gagtgggacg gattcgacga gtccaattat gaggagctgt tcgacgacga catcaaggct
     2461 gcgctcgcgc agctctctga tgaattccgc atggtgattc tgcttgcgga cgttgaggga
     2521 ttcgcttaca aggagatcgc cgatatcatc gatcggccga tcggcacggt gatgtcgcgc
     2581 ctcttccgcg gccgccgcat gctgcaaaag gttttggaaa aatacgcccg caacgagggc
     2641 tatattcgct caaagaaggc gtttaactga tctggaatcc tatgaataga tcttttgttc
     2701 tttttctgct tttactgttt ggtgctggcg ggcttgcagc ccagggcttt gtcgaaccgg
     2761 aagtcctgac cgccgaggct tttatctccc aaggccaggc ggtgcccggt tccgggctcg
     2821 aggccgtcgt cgtggtcaag attgtgcccg gtttgcatat caactccaac accccggccg
     2881 aagagtttct catccccacc cagctgcagt ttgccccctc tccctccctg agtttcggtc
     2941 cggcgctcta tcccaggccg ctcctgaaaa aattcgcctt ttccgataac gagctttcgg
     3001 tattcgagga ggaggtgaaa attcacaccc gtgtgcaggt gctgccgcaa gccgccccgg
     3061 ggaggctgca gatcgagggg acgctcacgt accagggctg taatgaaacc agctgtttct
     3121 cgcccggcga gaaaaagttc atactgcccc tggagatcgt cccggcgggc acgccggtgg
     3181 cacaggacac ctccgctcta ttttcagccg gtgtgcagga tcccggccgc ggaatccggc
     3241 cggcagcggc agccggggag gggggcctcc agctcacccc ggatgaaaga cgggcgatgg
     3301 agatcctcga gcacgggctt ttttacgcca tcgccgcctt tctgctcatc ggtctcgcgc
     3361 tcaatctgac tccctgtgtc tatccgatca tccccctgac cgtgagctat ttcggtggtc
     3421 agagcggccg cagccggggc tcgagttttg tcaatgccct tttatatctg atcggcatcg
     3481 ccctctcttt cgcgctgctc ggggtgcttt ccggtctggc aggaaagcag tggggttttc
     3541 tgtttcagag tccctggttt gtcgcagtgg tagccacgat cattctgctc atggcggcga
     3601 gcatgttcgg ggcgttcgag atcagcgtgc ccaccggctt gttgacgcgt ttcggcgggg
     3661 ctcgcgaggg gctgttgggg gctctgatca tgggactgac ggtcggggtg atcatcgcgc
     3721 cctgcgcggc agggatcatc atcggcctgg tcggcctggt cgcgaagctg ggtctggtgg
     3781 ccaagggcgg cctgctcttt tttgccatgg gcatcgggct cggcctgccc tatctggtgc
     3841 tggccacctt cagcggtctg ctcaaccgcc tgccgcagtc gggcatgtgg atgctttggg
     3901 taaaaaagtt ctttggtttt ctgctcatcg gcgtcgcgat ctatttcatc accccgcagc
     3961 tcgatctgat ccatgacaag ctgcttttcc tgctcgggct gctcggggtc gtcggcggcc
     4021 tgctgctggg cttcatcggc cacgaggagc agacccgggg attcaggttc ttccgacgcc
     4081 tgcttggggg agtgttgatt cttctcgggc tgcactggct caacgaggcc atccattcca
     4141 gaccctcgga gctgccatgg gtgcagtacc aggggcaggg catcgaagag ctgacagcgg
     4201 agggcaagcc gatttttatt gatttttatg cggactggtg cgccccgtgc aaacagctcg
     4261 acaaggagac cttttcggac agccgggtgc aggagcagtt tgccggcttt accattctca
     4321 aggtcgactg caccaagccg gatgcaaaga ccagggcctt catggggcgg tttgcagtga
     4381 ccggcatgcc gactctgctc tttctgaccc ccgcgggaga agagatcgct gatctgcgcg
     4441 aagttggttt tgtctccccc gagaaatttc ttgccagttt gcagcgggcc ctgcaggccc
     4501 gatgaccgtt tccccggggg gtgctgactg gcgccctccg cgggactctc ccccatttct
     4561 ccctgatgag cagtttgagt gtaccttgtg atggccaatg gctcctgtcg gctgtttgac
     4621 tgaggcatct cctgtggaac taaaaaaagt aaaattcatt cataatccca aatcggggct
     4681 ggtgcgcagc ccgcgcgtca tccgcagggc gatcgaggcg gtgctcgaag aggccccgtt
     4741 tgtgtatgat ttctccgaga ccgaataccg ggggcatgcc catgagctgg cccggcgggc
     4801 ggtggaggag gggtatgatg cagtggtcgc catcggcggc gacggcaccg ccaatgaggt
     4861 ggggtcggcg ctgctctaca ccgataccgc tctcggcatc atcccggtcg gttccggcaa
     4921 tggcctggcg cgcggactgt cgattccgat cgcgatccgg cgcgcagcca ggctgctgct
     4981 gaccggccgg atccggacta tcgataccgg gaccatcgag gagcgcaatt tttttattgt
     5041 caccggtctc ggttttgatg cccttgtcgg caagctcttc gatgatcgca gcctgcgcgg
     5101 acccctgcct tatttctaca tcggggtgcg cgaatttcta tttttccggc cggagacctt
     5161 cagactcgag ttcgacggac gcacagtcga gcgcagcgcg ctcctggtga cggtcgccaa
     5221 taccacgcag tggggcctgg gggtgatcat ctcgcccaac gcccggccgg acgacggcct
     5281 gctcgatgtc tgtatcatcc gcaacatcac ccttctgcgg gcgctctttc atctgcccaa
     5341 gcttttcacc ggcaagatcg acaaggtccg ggagtatgag cgctaccaga ccacctccct
     5401 gcgcatttcg agaaacaaac ccggcccctt tcatgtcgac ggcgaaccgg tcgatgcggg
     5461 ccaatcggtg caagtgggga tcaaggccca ggctctcaag gtgatcgtcc cctcccgcaa
     5521 gaccaggccg ctgacggtgc gggcgatttc ccaggcccgc cgcctgcaaa gagcgctgct
     5581 caatccgtaa gccctgctaa cggtgcggcg ctgccgcgct gtggggccaa agctcctcca
     5641 cctgcgagcg gcctgacgcg tcgcgtcccg gtggccccat ccgttgaatt ttttgtcctg
     5701 caattgctgt tgggtgaaac tttttacaga ctcaggagtc tgatagataa gatggtgtga
     5761 tcatggccga ggcgaggttt ttgtatgcat tggctgcaaa aaagtgcgat ggcttttttc
     5821 gtcctctctc tttcaagcct catttttttc attctcatca gcgacaggca gacccgcaac
     5881 agtgtgcttc gccccacgct tgaagccctc ggcgagcagc tgttcgcggc ggtgggagat
     5941 gacgtcgaca aggagcagct gcgcgccggc taccgggagt tcatccgtga ggccgaagag
     6001 cagcagatcg actccgcgag ggtcgaaaaa gtggctgcgg aaattctcaa cctgagccgc
     6061 cgggattccc tgatctcgtc gcgcgatgcc ctgcgcatcc tcgaaaaggc gcagatggcc
     6121 gtcgtcattc ctgccgcgcc tcccgccaca aaacaggctc aagtccgcga cccggatcgc
     6181 gaacggcaca cctggatcgc cggacgttcc ctgccgccca gctgggacaa ggaggagatg
     6241 gccgtgcgtc tgaaggcgat gcaggaattc cagcgccaga tggaccgcat cgcccgcgaa
     6301 tcggcccagc ttaagactct cgaagaacgc tatcgtttcc agcagaccga cagtggtttg
     6361 cgggttttaa tggattcgca ggttgtggaa actctggcca agcgtgacac cctgatgtgg
     6421 cgtaaactcc gcgaactgga gaagaagcag tggttgatgt gggggccgcc tcctccgcca
     6481 ccgcatcagg gtcgtgaaga ttcactccgg gccggacgta tctttttcca tcctcaagag
     6541 cgcgatatca cccggcggga gccctgagcc ctctctcgtg gcagcctgtt ttctcaccgc
     6601 gcgcgtaaat attccttgaa ttttatcccc gcatttccta ttatctgatc gctccccatc
     6661 tggagagtgt gctcatcctt ccgctttatc ctgatctggt gccgcgaact gaggggatcc
     6721 ttcatgctcc attctgcaca aattcgcatg caggcgataa gggagcagct agcgcaaatt
     6781 gcccccggcg atgagaccct gatgcgccgt ctcaacctgg tgcagcttga ccttcttctc
     6841 acccatcttt tcgagatcga ttacgatcct ccccgttatg taaaaaaatt ttcaaccctg
     6901 ctgggagaac agcttgagct tccccaggcc tgcctgctgc tggcgcagca gccctatgcc
     6961 cggcgccaga tctactacct gcagtccaag ggctgcaagc cggatcttgc cgtcgacacg
     7021 atcgtgcagg agttcgagcg ccggcggaaa gcgcctctcg tccggaaact gcagcccggg
     7081 gatgccatgg cctgcggtat gaacggctcc gttcagtatc tcatgatcgt gcccgtcgca
     7141 tccggggaga gaagcgacag ccggctcggc caccttctgt tctggttcaa cagcaccagg
     7201 cgggtcaccg attatgaacg catcatcggg cgcttcgctc gcctgctcgc cgaatccatc
     7261 gtccggcaga ggctgaacaa ggtgcgcgac cagctggccg ccgccgaccg ttttgagagc
     7321 aatcagatca agaaggtcat tcaaaaatcc ctgaccgccc tgcaggcggc catgggctgc
     7381 gattattcgg tggctctggt gttgaaacgt gccatcaccg acctcaacaa cgatccggcg
     7441 cgcgccgggg tgcagaattg ggaggtggtg accgaatatc tcaaaggcaa gaccaggctg
     7501 gccaaaaacc gcagcaaggc gaccggcctc tggatcgagg agagtgaagg cttgctcgca
     7561 ctcgccctag cgcactggcg ggaagatccg aaaaaggcca agaagggact cacttttcac
     7621 cagctcgagc ccggcagcag catgtatctc tgcgttgaaa caggtgagaa gggggcgggc
     7681 ctgcgcggct gcgaggacga gatgcatgag aagaccctgc actatatgcc gctggtgcac
     7741 gagggcaacc tgatcggcta tctcaaggct tcctaccgga gcaaaacacg tctgaccgag
     7801 ctggaccgga tcatcctcat cgatttcgct gactttctcg ccgagcgcat tcacaattcc
     7861 ttcatctact ccctcaccgt cgatcagacc cggtttctgc aaaagatcaa atcgatgtat
     7921 gagggcgccg accgcctgga ggtgagaaat ttcgccgacc tcggccaatt cctcctcgac
     7981 ctgacccagt tgatcactga gaccacccag atcgacaccg tggcgatcgg ctacctgacc
     8041 aaggatgtcg atggggaaaa ggtgctgcga ttcccccatc ccagtggctg ggatgagggg
     8101 gcgagccagc gcttcaggga gctgccggtc accgagggtt tagccggact cgccgtccgg
     8161 gcccgcagga atgtctttct ggcgccggtg gaagaacccc agaatccctc agatccggag
     8221 ccgacggtct atgtggatga aaaccgttcc cgtttcgtcg atgtccgcaa acatcccagc
     8281 tacctcaacc acgcaggcta ccgtaagctc tccagctatt acatcaaggt gcgcgaggag
     8341 aaggtctacg ccaactatat cttcgttatc gaattcaaga atatcgtcct cggggtcatc
     8401 gatgtggagg tcgcccagaa caattgggac cgcttcatgg gcatcggtta tctgcatttt
     8461 ttccggaccc tggccagcat gctggcgctg ctatttttcc gcgtccgcct cgccgaagaa
     8521 cacacccgca tggttcaggc ggtcaagtgg ttgagcgacg cggtttatat gtcgccgacc
     8581 tcggtggtta aaacgatatt ggccatgctg cagtccatat acggcgtcga aaaggccaaa
     8641 atcatcccgg gtgacctctt caaggagccg atcgagatcg atctcctcga ggggggcgtc
     8701 gagaattacg cccatctctt cctcggttat gaggccgagg agatccggcg tttcaccgtc
     8761 cccggcgacc gcatcgacac ggacaatctc tttctgctcg agttcaaaaa tcatctgcag
     8821 aattcgaatg atttcttgat gtacatcccc gacatcgagg agcatcagcc gcgcttcttt
     8881 gacaagcggg tggtggaaaa attcgaacgc ttccgcagcc ggccgcgctt ttacagctat
     8941 ctggccatcc tgctgggcga caagagccgg agcctggggg tgctggaact ggtatcccag
     9001 aaggtgaatg cctttgatga actgctgatc gatcagggat ttgtggagac ctggacctcg
     9061 cagatcagca tggcgctgcg ctacgccctg ctcgaagaga aacgagaggg cagcgcagag
     9121 acggtccagc aagccgagcc ggcgcccatt atcctccggc agggtaaggt tgtgcctttc
     9181 attgcagctg cgcagatcga gcatggctgt gatcttgtcg gtgttggcaa ggccatgacg
     9241 gagctgcgca gccagatcga gtccgccgcg ctgacctcat ccgtgcccat tctcatacat
     9301 ggcgagagcg gcacaggcaa ggatttggtc gccaaggcga ttcaccgccg cagcggctgg
     9361 gctcaggacc gcttcatcac ccaggattgc ggggctttca gccgcgagct ggtcgaggat
     9421 gccctgttcg gacatgtccg cggcgccttt accagtgcga cggaggaacg caagggcctc
     9481 ttcgaatgcg cccatggcgg cacccttttt ctcgacgaga tttccaacat gtctctggag
     9541 ctgcaggcca agtttctgcg cgtgcttgag gatgcgcgca tcacgcccct cggcagcagc
     9601 aagagcgtcc aggtgcaggt gcagatcctg gcggcgacca atgccaatct gctggaaaag
     9661 atccgtgccc gggagttccg tcaggatctc tactaccggc tggcggccat caccatcaac
     9721 acccccccct tgcgcaggct ggtggaggag gagcccacca atctcctgtt gttcatcagc
     9781 cattttgccg ctttgggtct gatccccgcc tccttcaccg ggatcgccaa gaaatccctc
     9841 gaggccctga tggagttcga ttttcccggc aatgtccgcc agctgcgcaa tctgctcagc
     9901 tcggccgcct tttccgggcg ggacaaggtc gttctcgaga ttccggagga ggctctcgcc
     9961 cccgatccag aggcccaact cgaggatgac cagtcccatg tccttgagga ggtcgaggat
    10021 tttatccaca agtaccaacg catcggccgc tttgagcaca ccccggagca ggtgatggcc
    10081 gggatccgga gcgccctgcg ttccgccggg gatgatctgg agaagatcag cagcttcatc
    10141 gatgattttg gcaccatcga tgtgccgctt gcgggactgc tcgccggcgt cggtcccacc
    10201 tgggcgcggc ggctgttgaa ggagcgcctc cggctcaagc tccatccccg cacctcgctc
    10261 tatttccgtg aaatcccctg atcgcaccct ccgctctgcc gagtgcgcgc actcatcccg
    10321 cagtgtgcgc gcactcctgc acatccccga atcagcgcat ttccgccact attgtataac
    10381 ccctttaatt tcaattttgt gattggcgcc gccagcggcg caaggatctg gcacactcgt
    10441 tgcaataggc ggggcagtgg aagatgctcg acccccggaa aggaggggtt atggaaaatg
    10501 aactcttact cagtctcctg gtgtttgcag gttgtgcggc tttgggcggt ttcatggcga
    10561 gggtgattat ccgctgtgcc gtggcggcga tcgtcggcct gggcatttac gccgtactgg
    10621 cacacgcgat gggcggggct gccaacagca tttcccgcgt gatcggggtg gctgtcggtc
    10681 tttgttcggc gctctattcg ctcatcgagg gtctggtcca tgcaccgggc gtcattggcc
    10741 tggtgatcgg ggtgatggcc gggttgcact tctggaaggt tgagagggag cgggatcatg
    10801 gcatcgtgcg gtatccccgc tatccgtcgc cagaatgatc ccgcggagtg gaggtaatgt
    10861 tcatttgagc agaacgatga tgaaggcggc cagaccgagg atgagggcga tggcagcgag
    10921 ccagagcagt gcggtgatgc gggcggccat gaactcgagc ccttcggcca gcccctgctg
    10981 ctgttcggcc atctttttga ccagttccgc ctggatgcgc tcgttttcct cgagttcttc
    11041 cacccgcaag gccaggccgc gcggggtgtc agcgacctcg atcgggacga tcgatggccg
    11101 ccgcgaggcg ttgagaagcc cccgggcggt ttgtacaatg gtgggaagca tggtgatcac
    11161 ctgtttccag ggaacccggg tcaggagtgt tccgacgccc atttgaactc cctaatctgt
    11221 tgaagtttct tggattctag tcaaccgtcg atgttcggcc ggggccgccc acatcggcgg
    11281 tttttttatg cgggctcacg ccgcttctgc attcgaagat gcacattttt ccctattttt
    11341 ccaacacaat tttgcgcttt tgttttgcag ggattagttt cgtatattag ttccgattat
    11401 ccacaccaag aggactaaag ccgaggcgaa aatatgaagc tggtagaatg tgttcccaat
    11461 ttcagcgagg gccgggatgc ggccaaaatt gacaagatcg ccgaggcgat ccgcagcagt
    11521 gggggggtga aactgctcga tgtcgatcca ggcagggata ccaaccgcac agtcatgacc
    11581 tttgtgggtg aacccgaacc ggtgctggcg gccgcctttg ttgccatagc ccgggctgca
    11641 gagctgatcg acatggccgg gcacagcggc gcccatccgc gtatgggggc gaccgatgtc
    11701 tgccccttca tccccgtgcg cggggtgacc atggccgaat gcgcagagct cgcccgcagg
    11761 ctgggtgaac gggtcggccg ggagctcggc atcccggtct atctctatga ggaggcggcc
    11821 gcgcgcccgg agcggcgcaa cctggcggat gtccgcgccg gcgaatacga ggggctggcg
    11881 gagaaaatgg cccatcccga ctggcgcccg
//
LOCUS       DAOPWU010000078        11898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5502_length_11898_cov_4.838386, whole genome shotgun sequence.
ACCESSION   DAOPWU010000078 DAOPWU010000000
VERSION     DAOPWU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5502_length_11898_cov_4.838386"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PKM23_05850"
     CDS             <1..1252
                     /locus_tag="PKM23_05850"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015843936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNY91034.1"
                     /translation="EIRFLVTAYVEGTIPDYQMSAWLMAVYLRGMDARETAALTQVML
                     DSGERIDLADISGRKIDKHSTGGVGDKVSLILAPLMAAAGVKVPMISGRSLGHTGGTL
                     DKLESIPGFKTELTPAAFHDQVRDLGVAMIGQNERLVPADKRIYALRDSTATVASIPL
                     ITASILSKKLAEGIDGLVMDIKCGRGAFMTEISRAEALAQSIIHTAALHSLPTVAVIT
                     DMNQPLGYAAGNWLEVREVLQALQGRGPEDLMQVTYALGVQMLLLAGFEGSTREAVNL
                     LQNTISSGRAMTRFQQLVRAQGGDVQSLQEPGSCAAARIQQHLRAGRSGFVTGIDALK
                     IGEAVVRLGGGRKALGDQIDPAVGVLLGKKTGDQVDKGEILAVIHANQTSAAREALEM
                     VRSAFTIEAAPAPKGKSILRIIKG"
     gene            1376..1448
                     /locus_tag="PKM23_05855"
     tRNA            1376..1448
                     /locus_tag="PKM23_05855"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1409..1411,aa:Asn,seq:gtt)
     gene            1524..4826
                     /locus_tag="PKM23_05860"
     CDS             1524..4826
                     /locus_tag="PKM23_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNY91035.1"
                     /translation="MKSWTMTGLLLVLLCLPAAAQIQILLDNDTPACTLVGTWNEKIS
                     SDAVNGQARYKVRTTTGANYALWQVPIKWPGSYVLEANLIKGNYADSVFYTIHTAAGD
                     TTVVISQNYLQGRQKLGEFSLAGQCWIKMTDKAAGKGNMVFADAIILTANIATWPLSG
                     QVRFSDNNPLITGRLTLSPAGGGATLIEKILTPDAREFLFPGVVDGWYRLSCSAYGYE
                     TALLDSIHVQGQAVTAVELVMVPVAGARSTISGTVRFQDGDPLRSGRLELFAQGDPLL
                     VASAQLLHDTPWSISGLLPGRYRLLFTAPNCLTDSTTYAEVLLGESDLQLEPLILYPF
                     FRFAWLTDSHVGSGNDDALQIVLRRIALMKEGIDFVIHTGDISEKGLESEFALYKSYE
                     SLCQLPVYNIPGNHETKWSPSGLQAWTRKFGERHFTFTHKGFKFIGLNNGIPMRGGCG
                     YIDPADIAWLKEELAGIDAATPVVFAIHLPLDVTNTPNYWEAVDLLKAHRTVWIMVGH
                     GHVNKSYNFEGIPGAMGRDTFSGDAGFNIVTLSEKEIRAQVCTAVDGVVAPAWYQTST
                     LQTVQPAISFTNLAKGSTLAGGTMIDISVSESVRNGQWEVSNSSLGLRALAGGGTAWQ
                     CELPASGIENGYHTLRVSFTTEGGKTIAATTSFFFENGHPKALWKYDCGAEVLTQPAF
                     DESGVYVGTSDGRIIGLSRSTGLALWPPVQCEGGVFSAPLLLDRMLYAGSADGALHAI
                     DARTGGQKWHFQVEDAVLSAPVAQDTLIFFAGGGAFYAVGRSAGQLVWKFDGCGTVEC
                     KPCLAGNRIIFGSWDGKVRALDTATGQQLWIWTRQANFYYAPAACWPVASADKLFVTD
                     PERYTSALDLATGRTVWSSKSPEVYDSIGISGDGSRLYVRSLDGSLYAFSTADAAQQK
                     FWQSDVGYGWDSTPSMPIEKEGVVYSAGKKCFVAANDGATGATWWKYWIGPTHATTVT
                     PIDRSHVLASSLDGVVLFIEGDPATRIREESSDALPQTSRLFPPYPNPFNSSVSITYT
                     LSSRQPVAVTIYNVRGEEVYSSPARDQAAGEHHFTWQGVDRSGRAVPSGIYYLRLQGS
                     DLVQTVKLALVK"
     gene            4930..7470
                     /locus_tag="PKM23_05865"
     CDS             4930..7470
                     /locus_tag="PKM23_05865"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNY91036.1"
                     /translation="MKKLQLTFLLLLAGAALMAQSGDFSMENYRRFLAETRSLTAAGL
                     ASRYPAGRFEASVPYDWRSALYADSIELKYLLTTGEKQLLQKNGFMVSERLSHTTFIE
                     AMADIFHKDLPLFISADAILHAVHISYDQILKRMEQVHLIPQLEVLLKALHDGVPAMA
                     ARYSGQPEMRPMVEDVDLYLAVARHLLDQTTRPIYKQNHSAFSEVLALIDGLKPASYP
                     LFGSSPRLIDFSQFQVRGHYADEFIPELGCYFRAMIWLGRTEFYLIAPRSSEIPPPFA
                     DLQRQIIDALLLDELLDQSGARETLAGIDGLLAFFVGECDNVTPDNLTELSRRLAITT
                     PAQLLDSLQTVAFQENLAASSFAWQRINTQMLYSDPLDPESIRPASSFLLLGQRFFID
                     SYITGQVVYDKITHDGVKVTRMLPSTLDVLFGLGNDAAGQLLQAELEQYHYAPKLAAV
                     RRLIDGYDDGFWTSSLFNHWLTAIRALNPPADRSTLPPALHTAAWWQRMMNSQLGAWT
                     ELRHDNLLYAKQSYSGMVICSFPHVYIEPIPELYDALARMARAGAARFSGTSYDALIA
                     DYFSSSAATFDTLGAIAGKLEQGATLAEKETLFLQRTLRQSMEGCGGPTFTGWYPRLY
                     LYYDSDGMTRKDYLVADIHTAPTDEFGAMVGWVKHVGTGPINLGIFVLPLAGRNIAFT
                     GPMMSYYEHLATNFRRLTDEEWAEIYSLAPSLRPDFVHPYLADQKGAARGGGTMLLTG
                     VEERPEAAGGPATPLLAANWPNPFNASTLIRITIPSGGHASSARLAVYDRRGRLVRLL
                     FTGQAAPGHYLARWDGRDEGGRPAPSGIYFYRFTQGEQVVEGRMSLVR"
     gene            complement(7657..8055)
                     /locus_tag="PKM23_05870"
     CDS             complement(7657..8055)
                     /locus_tag="PKM23_05870"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNY91037.1"
                     /translation="MATRFTTTLYVRSYELDSFGHVNNAVYLQYLEYARSEYLLQVGL
                     SFNDFQTWNAIPYVVRAEIEYKSSSRCHDELEITGWVSAWGKSSFVLSYEIFNRSTGK
                     MGALAEVKFAWVTREEKIIRIPQIFRERMT"
     gene            complement(8066..10405)
                     /locus_tag="PKM23_05875"
     CDS             complement(8066..10405)
                     /locus_tag="PKM23_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91038.1"
                     /translation="MSRGVRIRTGLILLLLIGLSAGPAAGQKQGRAAPAKHGRVAGFV
                     VDAAGKTPIPQANVMLANTSLGAVSGRNGAFYIERVPPGTYQLVVTMLGYRRMIIKDV
                     VVRAGEVRALRVSLNRQAIDMGKVQVEAKREAQAFQYEKSIAGHEIITPRFIARRPGA
                     LEDAYRALNVLPGVTARSDLNTQLYIRGGSPDQNLFLYDGIEITTPGRLFIIMGGGIS
                     LVNPDIVQGIDLEPGGFDASHGDKTSALLQIVNREGRRDRTAASASLAMVTARAVAEG
                     PIGKSKGSWLVAGRRSFYDLFANRLYEKDYIFPYYYDLHAKTAYDLTRSSRLTAFYTH
                     LGEGAKMMNVESEQLGLLNSGLGHIAGLRLNSLLSSKVMADLLLGYYEDRNDIRMFDT
                     YNARYHANLQYGIRRRTAKGELSYEATPRISFKGGVQFNNHATDLDGAIEWRNEIPLL
                     DFDSLRLAIRSEDLGAFTQLRLLPRNWLEVNAGVRYDYSSLYNEYLWHPRVKLLLFPK
                     RRANIWLSYGSYSQYPDIMTVIGRGEPLDIGSNPENIGAERAEHRIIGMQYAPSAGSL
                     LKVELYRKDMTDLLVNPNSLLFIPENSGTGLARGVEFSFQHTRQAEERFGWWANLALA
                     EAKYKRFGGDEWRYFDNDQRLQASAGAEWRISKGWQCSLIGHYNSGFPYTPIRAIQRD
                     LEAPREPLYNYAIINKAHNSARYPYYLRCDVRLGYLRESRGRTFSAYLDLINVLNRKN
                     LYLYDWRYEKSSDDSDGYVRRSVIYMMPFLPSFGVSFSF"
     gene            complement(10398..11330)
                     /locus_tag="PKM23_05880"
     CDS             complement(10398..11330)
                     /locus_tag="PKM23_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91039.1"
                     /translation="MKIAPSSPPPARVHHLPRILALAAVLFLFTCSEQTTVEYETLIP
                     ALRTLAGEEGWSASYPAWHAGGRQLLYCAQPKAGGAVTELREVEVESRISRTLLTDST
                     RLRFPSWSPSDSTILCTSARSGSHDLWLFNPATATWRRLTSLAGNESFPRWSPDGDRI
                     AFLSLGRIALLDPATEEISYLVTPFLIVLSLAWSPDGESLLFSADNGSGEYLYRFDLS
                     GSRFEELFPISQKGSWPVAAPAAAEGAGEHIAWRASTGPGTAGIYFCPAGAEKPSLVV
                     RDGAMPAWSPDGTALVYIRGTNLVWEKVWIAIDE"
     gene            complement(11362..>11898)
                     /locus_tag="PKM23_05885"
     CDS             complement(11362..>11898)
                     /locus_tag="PKM23_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNY91040.1"
                     /translation="RVLDRLIALNDSLWFDDVRTPGLETLDSQLAAAFYAAIDSLTLR
                     YGDNPGMWDWGSVHTLTQPHPFGRHTPFRRYFNIGPAPSAGGNHTLNNSTYSMAEPFA
                     TIIGPCVRQISDMSTSEWHVILPAGQSGHPFSRHYRDQQPLWQEGEMITLQLNSLARR
                     NPRWNWQILRPVSANSRP"
BASE COUNT         2218 a         3813 c         3633 g         2234 t
ORIGIN      
        1 ggagatccgc tttctcgtca ccgcctatgt tgaggggacc atccccgatt atcagatgtc
       61 cgcgtggttg atggccgtct acctgcgcgg catggacgcc cgggagactg cggcccttac
      121 ccaggtcatg ctcgactccg gtgaacggat cgatctggcc gatatctcag gccgcaagat
      181 cgacaagcac agcacgggcg gcgtcggcga caaggtctcc ctcatcctcg cccccctgat
      241 ggcggcggca ggggtcaagg tgccgatgat ctcgggccgc agccttggcc ataccggcgg
      301 caccctcgac aaactggagt cgatccccgg ctttaaaacc gagctgacgc cggccgcctt
      361 tcacgaccag gtgcgcgacc tcggggtggc gatgatcggc cagaacgagc ggcttgtccc
      421 cgccgacaag cgcatctacg ccctgcgcga cagcaccgcc accgtcgcct ccatcccgct
      481 catcaccgcc agcattctca gtaaaaaact cgccgaaggc atcgacggcc tggtgatgga
      541 tatcaagtgc ggcaggggag cctttatgac cgagatcagc cgcgccgagg ccctggccca
      601 gagcatcatc catacagccg ctctgcacag cctgccaacc gtggccgtga tcacagacat
      661 gaaccagcct ctcggctacg ccgccggcaa ctggctcgag gtgcgcgagg tgctccaggc
      721 cctgcaggga cgcggaccgg aagacctcat gcaggtcacc tacgccctcg gggtgcagat
      781 gctcctgttg gcgggcttcg agggcagcac ccgcgaggcg gtgaacctgc tgcagaacac
      841 catctccagc ggcagggcga tgacccgttt tcagcagctg gtcagggccc agggcgggga
      901 tgtgcagagc ctgcaggagc cgggcagctg cgcggcagcg cgcatccagc agcatctccg
      961 cgccggcagg agcggatttg tgaccgggat cgacgccctc aagatcggcg aggcggtggt
     1021 ccggctcggg ggagggcgca aggccctcgg cgaccagatc gatccggccg tcggcgtcct
     1081 gctcggcaaa aagaccgggg accaggtcga caagggggag attctggcgg tgattcacgc
     1141 caaccagacc agcgccgccc gtgaggccct cgaaatggta cgcagcgcct tcaccatcga
     1201 ggcggcgccg gcgccgaagg gtaaaagcat cctccgcatc atcaagggtt gactcgtccg
     1261 cactgttccc tcccgcaggt gtggggtggg agccagattt gcggctgccc tgcgcaaata
     1321 taatcttgac ttttaccgag gttattgata tattatcacc gctaaatttg ttcattcagg
     1381 tgtagctcaa tcggcagagc gggtggctgt taaccactag gttgtaggtt cgagtcctac
     1441 cacctgagca aaggcaggct ttcggaagga agcctttttt tttgcccctc ctctacatcc
     1501 cacctttaaa caggagacgc gtgatgaaat catggacgat gaccggactg ttgcttgttc
     1561 tcctctgctt gccggctgca gcacaaattc agatcctcct cgacaacgac acccccgcct
     1621 gtaccctggt cggcacctgg aacgaaaaga tcagcagcga tgcggtcaac ggacaggccc
     1681 gctataaagt gcgcaccact accggcgcca attacgccct ctggcaggtg ccgatcaagt
     1741 ggcccggcag ctacgtcctc gaagccaatc tcatcaaggg caattacgcc gatagtgtct
     1801 tctacaccat tcacaccgcc gccggcgaca caaccgtcgt catctcccag aactacctgc
     1861 agggcaggca aaaactcggc gagttctccc tggcgggcca gtgctggatt aaaatgaccg
     1921 acaaggctgc cggcaaaggc aacatggtct tcgccgatgc catcatcctc actgccaaca
     1981 ttgccacctg gcccctctcc gggcaggtcc gcttcagcga taacaatccg ctcatcaccg
     2041 gtcgtctcac cctctccccg gccggcggtg gtgcaaccct gatagaaaag atcctcacgc
     2101 ccgacgcacg ggagtttctc ttccccggtg tggtcgacgg ctggtaccgt ctgagctgct
     2161 cggcctacgg ttacgagacc gccctcctcg attccattca tgtgcagggc caggccgtca
     2221 ccgctgttga actggtcatg gtcccggttg cgggcgcccg ctctaccatc tccggcaccg
     2281 tccgttttca ggatggagac cccctccgca gcggacgatt ggagctgttc gcacaaggtg
     2341 atcccctgct ggtcgcctcc gcacagctgc tgcacgacac cccctggtcc atctccggcc
     2401 tgcttcccgg ccgctaccgc ctcctcttca cggcgcccaa ctgcctgacc gacagcacca
     2461 cctatgcaga ggtcctgctt ggtgaatccg atctccagct ggaacccctc attctctacc
     2521 ccttcttccg cttcgcctgg ctcaccgatt cccatgtcgg ctcgggcaac gatgacgccc
     2581 tgcagatcgt cctgcgccgg atcgccctga tgaaggaggg gatcgatttt gtcatccaca
     2641 ccggcgacat cagtgaaaag gggctcgaaa gcgaattcgc actctataaa agctacgagt
     2701 cgctctgcca actcccggtc tacaatattc ccggcaatca cgagaccaag tggtcgccct
     2761 cggggctgca ggcctggacg aggaaattcg gggagcgcca tttcaccttc acccacaagg
     2821 gcttcaagtt catcggcctc aacaacggca tccccatgcg cggcggctgc ggctacatcg
     2881 atcccgccga tatcgcctgg ctgaaggagg aactggcggg gatcgacgcc gccacgccgg
     2941 tggtcttcgc catccatctg cccctcgacg tcaccaatac ccccaactac tgggaggccg
     3001 tcgatctgct caaggcccac cgcacggtct ggatcatggt cggccacggc catgtcaaca
     3061 agtcatacaa tttcgaaggc atccccgggg ccatggggcg cgacaccttc agcggcgatg
     3121 ccggtttcaa catcgtcacc ctctccgaga aggagatccg cgcccaggtc tgcaccgccg
     3181 tcgacggcgt cgtcgccccg gcctggtacc agacctccac cctgcagacc gtacagccgg
     3241 ccataagttt caccaatctg gccaagggga gcaccctcgc cggcgggacg atgatcgaca
     3301 tttcggtgag tgaatcggtc cggaacgggc agtgggaggt gagcaacagc agcctcggcc
     3361 tgcgcgccct ggcgggcggc ggcaccgcct ggcagtgcga actgcccgcc tccggcatcg
     3421 agaacggcta tcacaccctc cgcgtctcct tcaccacaga ggggggcaaa accattgccg
     3481 cgacgacctc gttcttcttc gaaaacggcc atccgaaagc gctgtggaaa tacgactgcg
     3541 gcgccgaagt gctgacccag cccgccttcg atgagtcggg ggtttatgtc ggcaccagcg
     3601 acggccgtat catcggcctc agcaggagca caggacttgc tctctggcct cccgtccagt
     3661 gcgagggtgg ggtcttttcg gctcctctcc tcctcgaccg catgctctat gcgggctccg
     3721 ccgatggggc cctccacgcc atcgatgccc gcaccggcgg gcagaaatgg cactttcagg
     3781 tcgaggacgc cgtcctgagc gccccggtcg cccaggatac gctgatcttt ttcgccggcg
     3841 gcggcgcctt ttacgccgtg gggcgttcag cgggtcaact ggtgtggaaa ttcgacggct
     3901 gtggcaccgt ggagtgcaaa ccctgcctcg ccggcaaccg catcattttc ggctcctggg
     3961 acggcaaggt gcgcgccctc gacaccgcca ccggccagca gctctggatc tggacccgcc
     4021 aggctaattt ctactatgca cccgccgcct gctggccggt cgcttcggcc gacaagctct
     4081 tcgtcaccga tccggaacgc tatacctccg ccctcgatct cgccaccggc agaaccgtgt
     4141 ggtcctccaa aagcccggag gtctatgatt cgatcggcat ctccggggat ggcagccgcc
     4201 tctatgtccg ctccctcgac ggctccctct acgccttttc gaccgccgat gcagcccagc
     4261 aaaagttctg gcaatctgat gtcggctacg gctgggactc gacgccctcg atgccgatcg
     4321 aaaaggaggg agtggtctat tcagcgggca aaaagtgttt cgtcgcagcc aacgacggtg
     4381 cgaccggcgc cacctggtgg aaatactgga tcggaccgac ccacgccacc acggtcacgc
     4441 ccatcgaccg cagccacgtc ctggcctcct ccctggacgg cgtcgtgctt tttatcgagg
     4501 gtgatccggc cacccgcatc cgggaggaga gcagtgatgc gctgccgcag acgagcaggc
     4561 tttttcctcc ctatcccaat cccttcaaca gctcggtgag catcacctac actctttcca
     4621 gccggcagcc ggtcgccgtc accatctata atgtgcgcgg cgaggaggtt tactcttcgc
     4681 cggcccggga tcaggccgcc ggcgagcacc actttacctg gcagggcgtc gaccgcagcg
     4741 gcagggccgt cccctccggg atctactatc tgcgcctgca gggcagcgat ctggtccaga
     4801 ccgtcaagct cgcactggta aaatagtact tgcctgaggc ctctattttg gttacgttag
     4861 aatagaggcc tcatttcctt cctcccccgc cgcctcgtca agggcctccc ctttttgagg
     4921 agtcgcgtca tgaaaaaatt acagctcacc ttccttctgc tgctcgcagg cgccgccctt
     4981 atggcccaga gcggcgattt ttccatggaa aactaccgtc gctttctcgc cgaaacccgg
     5041 agcctcaccg ccgccgggct ggcatcgcgc tatcccgcag gccgcttcga agcctccgtc
     5101 ccctacgact ggcgcagcgc cctctacgcc gacagcatcg aactgaaata cctgctcaca
     5161 actggagaaa agcagctgct gcagaaaaac ggctttatgg tcagcgagcg cctctcccat
     5221 acaaccttca tcgaggccat ggcggacatc tttcacaagg acctgccgct cttcatcagc
     5281 gccgatgcca tcctccatgc ggtgcatatc tcctatgacc agatcctcaa acggatggag
     5341 caggtacatc tcataccgca gctcgaggtt ctgctcaaag ccctgcacga cggggtgccc
     5401 gccatggccg cccgttattc gggccagccg gagatgcggc cgatggtcga ggatgtcgac
     5461 ctctacctgg ccgtagcccg gcatttgctc gatcaaacga cccggccgat ttacaaacaa
     5521 aaccactccg ccttttcgga ggtgctcgct ctgatcgatg gtctcaagcc cgccagttat
     5581 ccccttttcg ggtcgagtcc gcgcctgatc gatttcagcc agtttcaggt gcgcggccac
     5641 tatgccgatg aatttattcc ggaactgggc tgttattttc gtgccatgat ctggcttggc
     5701 cggacggaat tctacctgat cgcgcccagg tcgtcggaga tcccgccccc gtttgccgac
     5761 ctgcagcgcc agatcatcga cgccttgctg ctggatgagc tgctcgacca gtccggcgca
     5821 agagagaccc tggccgggat cgatgggctg ctcgctttct ttgtcggcga atgcgataat
     5881 gtcactccgg acaacctgac cgagctgagc cgtcgtctcg ccatcaccac tccggcgcag
     5941 ctgctcgaca gcctgcagac cgtcgccttt caggagaatc tggccgcttc atccttcgcc
     6001 tggcagcgca tcaacacgca gatgctctac tccgaccccc tcgatccgga gagcatccgc
     6061 ccggcctcct cttttctcct gctcggccag cgcttcttca tcgattccta catcaccggc
     6121 caggtggtct atgacaagat cacccacgat ggcgtcaagg tgacgcgcat gctcccctcg
     6181 acgcttgacg tcctcttcgg gctgggcaat gacgccgccg ggcagctgct gcaggcggag
     6241 ctggagcagt accattatgc ccccaagctg gcggcggtgc gccggctgat cgacggctat
     6301 gacgatggct tttggacaag ttcgctcttc aaccactggc tcacagccat tcgggccctc
     6361 aatccccccg ccgatcgcag caccctgccg ccggcgctgc acacggccgc ctggtggcag
     6421 cgcatgatga acagccagct gggcgcatgg accgaactcc gccacgacaa tctcctctac
     6481 gccaagcagt cctattcggg gatggtgatc tgctccttcc cgcatgtcta catcgagccg
     6541 atccctgagc tctatgacgc cctggcccgg atggcccgcg ccggcgcggc acgattctcc
     6601 ggcaccagct atgacgccct catcgcggac tatttcagca gcagcgcggc caccttcgac
     6661 accctgggag ccatcgccgg caagctggag caaggcgcaa ccctggctga aaaggagacc
     6721 cttttcctgc agcgaaccct gcgacaaagt atggaaggct gcggcggccc cactttcacc
     6781 ggctggtacc cgaggcttta cctctattat gattcagatg gaatgacccg gaaggattat
     6841 ctggtcgcgg atatccatac tgcgccgacg gatgaatttg gcgccatggt gggatgggtc
     6901 aagcatgtag gcaccggccc gatcaatctc ggcatattcg tcctgcccct tgccgggaga
     6961 aatatcgcct ttaccggccc gatgatgagc tactacgagc atctcgccac caatttccgg
     7021 cggctgaccg atgaagagtg ggcggagatt tacagcctcg ccccctcact gcgccccgat
     7081 ttcgtccatc cctatctggc ggatcaaaag ggcgccgccc gcggcggcgg caccatgctg
     7141 ctgaccgggg tcgaagagag gccggaggcg gccggagggc cggccacccc cctgctggcg
     7201 gctaactggc ccaatccctt caatgcctcg accctgattc gtatcaccat cccctccgga
     7261 ggccatgcct cctccgcccg gctggcggtc tacgaccgcc gcggccggct ggtgcggctg
     7321 ctcttcaccg gccaggctgc acccggccac tatctggcgc gctgggacgg ccgcgacgag
     7381 ggcggccggc cggcgccgag cggaatatat ttttatcggt ttacgcaggg tgaacaggtg
     7441 gtagagggca ggatgagtct ggtgaggtaa gcactgtacg attgattaaa acagcacaat
     7501 ggcaggataa acccggtgag gcaagtgctg cacggttgat caaaccggct tgagagcagg
     7561 atgagcccag caaggcaagg gcgctgatac tggctaaggc tgcaccaggg gccggtcgag
     7621 atcagcgcac aggatcccgc cggcctgctt ttccgtctac gtcatccttt cgcgaaagat
     7681 ctgcggaatg cggatgatct tctcctcgcg cgtcacccag gcgaacttga cctccgccag
     7741 ggcccccatt ttacctgtgc tccggttgaa aatctcatag ctcagcacaa aactgctttt
     7801 gccccaggcg ctcacccacc cggtgatctc cagctcatca tggcagcggg aggaggactt
     7861 gtactcgatc tcggcccgca cgacataggg aatggcgttc caggtttgaa aatcattgaa
     7921 cgagagcccc acctgcagca gatactcgct gcgcgcgtat tccagatact gcagataaac
     7981 cgcattgttg acatgcccga aggagtcgag ctcgtagctg cgaacgtaca gggtggtggt
     8041 gaagcgggtg gccatctttt ctcctctaga aagagaaact gacaccgaag gagggcagaa
     8101 agggcatcat atagatgacg ctgcggcgga catagccgtc gctgtcatcg ctgctcttct
     8161 cgtagcgcca gtcataaaga tagagattct tgcggttcag cacgtttata aggtcgaggt
     8221 aggccgagaa agtgcgcccc cggctctcgc ggagataacc gaggcgcaca tcgcagcgca
     8281 ggtaataggg atagcgcgcg ctgttgtggg ccttgttgat gatggcatag ttgtagagcg
     8341 gctcgcgggg cgcctcgagg tcgcgctgga tggcccggat gggagtatag gggaagccgc
     8401 tgttgtagtg gccgatgagg ctgcactgcc atcccttgct gatgcgccac tccgcgcccg
     8461 cgctggcctg caggcgctgg tcattgtcga aatagcgcca ttcatctccc ccgaagcgct
     8521 tgtacttcgc ctccgccagg gccaggttgg cccaccatcc aaagcgctcc tcagcctggc
     8581 gtgtgtgttg gaaggagaat tccacccccc gagccaggcc ggtgcccgaa ttctccggaa
     8641 tgaaaagcag ggagttggga ttgacgagca gatccgtcat atccttgcgg tataattcca
     8701 ccttgaggag gcttccggcc gagggagcgt actgcatgcc gatgatgcgg tgctcggccc
     8761 gctcggcccc gatattctcc ggattgctgc cgatgtcgag cggctcgccg cggccgatca
     8821 cggtcatgat atccgggtac tgggagtagc tgccgtagct gagccagata ttggcccgcc
     8881 tcttcgggaa aaggagcagt ttgacgcgtg gatgccagag atactcattg tagagcgagg
     8941 aatagtcgta gcgcacgccc gcgttgactt cgagccagtt ccgggggagc agtcgcagct
     9001 gggtaaaggc cccgaggtcc tcggaacgga tcgcaaggcg aagcgaatca aaatcgagca
     9061 gcgggatttc gttgcgccac tcgatggcgc cgtccagatc cgttgcgtgg ttgttgaact
     9121 ggacgccgcc cttgaagctg atccggggag tcgcctcgta gctcaattcc cccttggcgg
     9181 tgcgccgcct gatgccgtac tgcagattgg catggtagcg tgcgttgtag gtatcgaaca
     9241 tgcggatgtc gttgcggtcc tcgtaatagc cgaggaggag atcggccatc actttggagg
     9301 agagcaggct gttgaggcgc aggccggcga tatggccgag cccggaattg agcagaccaa
     9361 gctgttcgct ctccacgttc atcatcttcg ccccctcgcc gagatgggta taaaaggcag
     9421 tcaggcggct gctgcgggtg aggtcgtagg cggtcttggc gtggagatcg taataatagg
     9481 gaaagatata gtccttctcg tagaggcggt tggcgaagag gtcgtaaaag ctgcgccggc
     9541 cggcgaccag ccaggagccc ttcgacttgc cgatgggccc ttcggcgaca gcccgcgccg
     9601 tgaccatggc cagactggcg ctggcggcgg tccggtcccg gcgcccctcg cggttgacga
     9661 tctggagcag ggccgaggtc ttgtcgccgt ggctggcgtc aaagcccccc ggttcgaggt
     9721 cgatgccctg aacgatgtcc ggattgacca ggctgatgcc gccccccatg atgatgaaga
     9781 gacggcccgg cgtggtgatt tcgatgccgt cgtagagaaa gaggttctgg tcggggctgc
     9841 cgccgcggat gtaaagctgg gtgttcaaat cgctgcgcgc ggtcaccccg ggcaggacgt
     9901 tgagggcgcg gtaggcgtct tcaagcgccc cgggacgccg ggcgatgaag cggggggtga
     9961 tgatttcgtg gccggcgatc gatttttcat actgaaaggc ctgggcttcc cgcttggcct
    10021 cgacctggac cttgcccata tcgatggcct ggcggttcag gctcacccgc agggcgcgca
    10081 cctctcccgc ccgcaccacg acatccttga tgatcatccg gcggtagccg agcatggtga
    10141 cgacgagctg ataggtgccc ggcggcacgc gctcgatgta aaaggcgccg ttcctgccgc
    10201 tcacggcccc cagcgaggtg ttggccagca tgacattggc ctgggggatc ggggtcttgc
    10261 cggccgcatc gacaacgaaa ccggcgacac ggccgtgctt cgcgggggcg gcacggccct
    10321 gtttctggcc ggccgccggg cctgcgctca ggccgatgag gaggagcagg atcaagcccg
    10381 tccgtatgcg gacgcctcta ctcatcgatg gcgatccaga ctttttccca gaccagattt
    10441 gtgccgcgga tatagacaag ggcggtcccg tccggcgacc aggcgggcat ggccccgtcc
    10501 cggacgacca ggctcggctt ttcggctccg gcgggacaaa aatagatccc ggccgtgccg
    10561 gggccggtcg aggcccgcca ggcgatgtgc tctcccgcac cctcggccgc agccggcgcg
    10621 gccaccggcc atgagccctt ctgggagatc gggaagagtt cttcaaaccg gctgccgctg
    10681 aggtcaaacc ggtagagata ctccccgctg ccattatcgg ccgagaagag cagactctcc
    10741 ccgtccggac tccaagcgag cgagagaaca atcagaaagg gggtgacgag ataggagatc
    10801 tcctcggtcg ccggatcgag aagagcgatg cggccaaggc tgagaaaagc gatgcggtcg
    10861 ccgtcagggg accagcgcgg aaaggattca ttcccggcga ggctggtgag tcgtcgccag
    10921 gttgcagtgg ccgggttgaa aagccagaga tcatggctgc cgctgcgtgc ggaggtgcag
    10981 aggatggtgc tgtcggaagg actccaggaa gggaagcgaa gcctggtgga gtcggtgagg
    11041 agcgtccggc tgatccggct ctccacctcg acctcgcgca gttcagtcac cgcgcctcct
    11101 gccttgggtt gggcgcagta gagcagctgc cggcccccgg cgtgccaggc tggatagctg
    11161 gcgctccagc cctcttctcc ggccagggtg cggagcgcag gaatgagagt ttcgtattcg
    11221 acggtagtct gttcgctgca ggtgaacagg aagaggaccg cagccagggc aagaatcctg
    11281 ggcaggtggt gtacacgcgc cgggggtgga gacgagggtg ctattttcac agggtgcagg
    11341 ggcatgatta atattatagc gtcagggccg ggagttcgcg gaaaccgggc gcaggatctg
    11401 ccagttccag cgcggattgc ggcgagcgag gctgttcagc tgcagggtga tcatctctcc
    11461 ctcctgccac aggggctgct gatcccggta gtgccggctg aaggggtggc cgctctgtcc
    11521 ggcggggaga atgacatgcc attcggaggt cgacatgtcc gaaatctgcc gcacacaggg
    11581 gccgatgatg gtcgcgaagg gctcggccat gctgtaggtg ctgttgttga gggtgtggtt
    11641 gccgcccgcc gagggcgccg ggccgatatt gaaataacgc ctgaaggggg tatggcgccc
    11701 gaagggatgg ggctgggtca gggtgtgcac gctcccccag tcccacatgc cgggattgtc
    11761 gccgtagcgc agggtcagcg agtcgatggc cgcataaaag gcggcggcga gctggctgtc
    11821 gagggtctca aggcccgggg tgcgcacatc atcaaaccag agggagtcgt tgagggcgat
    11881 caggcggtcc aggacgcg
//
LOCUS       DAOPWU010000079        11693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5618_length_11693_cov_4.515810, whole genome shotgun sequence.
ACCESSION   DAOPWU010000079 DAOPWU010000000
VERSION     DAOPWU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5618_length_11693_cov_4.515810"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKM23_05890"
     CDS             <1..252
                     /locus_tag="PKM23_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016111220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNY91041.1"
                     /translation="PHEVLLVIDATTGQNGLSQARRFAEAVKVTGVIITKLDGTAKGG
                     IVFSIARELGIPVRYVGLGEKIDDLQSFDPQTFVEALFA"
     gene            249..1538
                     /gene="rimO"
                     /locus_tag="PKM23_05895"
     CDS             249..1538
                     /gene="rimO"
                     /locus_tag="PKM23_05895"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNY91042.1"
                     /translation="MKLHYVSLGCPKNSVDLETILGGLTGGMELVEEPEIADVALVNT
                     CAFITSAKQEAIETILQLAALKEERPGLKLLVSGCLPQRYRAELEELLPEVDRFFFDL
                     DAGVTSAGIAAYLGVAAHPCARRHRLTPRHYAYLRIAEGCDNRCSYCAIPLIKGPGRS
                     RPLAEIRTEAELLAADGMQEVMIVAQDSTRYGLDLGRSVRLHDVLAALQEVSRLRWIR
                     LLYSHPAHWYDELIEAVAALDKVVPYIDLPIQHISDPLLRAMGRKSSRGQIEALITRL
                     RSRIPGLALRTSLIVGFPGEGEREFQELCDFVRQIRFERLGVFSYSQEEGTPAETWPQ
                     SVSEEEKEARLQEIMAIQAELSLQNNRGLIGRRLQVLVDQPGEEPGTCLARTVWDAPE
                     IDNSVIVSGELAPGSFAMVRITGAAPYDLYAEPAGDD"
     gene            1635..2924
                     /locus_tag="PKM23_05900"
     CDS             1635..2924
                     /locus_tag="PKM23_05900"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNY91043.1"
                     /translation="MHKAIFAAVLMLAVTATRMQAQVEFSTMQEQSGPDFHVDLVNTA
                     VGPASRLSRLNVYLEIVYDELQFVKKEENFEATYEISVTIMDKDDDQADGEIWKELAT
                     ATSFDMTNERTRLSQSHRHFDLEPGHYKVNIAVQDNETHRGSTRSLSIDLRNYQESTM
                     RGSDILLVSEMQRDSTGAISIRPQVSDATKGIVDSTQAFFEVYCENPPQELTVSYELA
                     GKITHKRIRRSFTHTLNSWRNPVYFKLHPDSLPQDSYLLTVQIESAGGKIKREKPVYI
                     RWSALPATASDLKTAVEQLRLVASKDEWKRLKKAKGDALLTEFKAFWKRHDPSPGTDA
                     NETMDAFYSRVEFANQRFSVMQMPGWRTDMGVIFIILGSPDNVERDVYPRDMKPYEIW
                     TYYEFNRDFLFLDYTGFGDYRLQTPMSIYEFQRLLHN"
     gene            2936..3904
                     /locus_tag="PKM23_05905"
     CDS             2936..3904
                     /locus_tag="PKM23_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNY91044.1"
                     /translation="MELRIPPALVPGDRIGVISPASPPKPEKHDQYQNGLAYLRGLGY
                     KISEGKHVLDQYGYLAGTDESRAADLNAMFRNPRIKAIICSRGGYGTPRLLDLIDYEA
                     VRKHPKILVGYSDITSLQLALFARTGLVTFSGPMVAVEMGKGILPFTEKHFWAMVGKS
                     TRKLLKAGAGEYEPRIYHGGVGEGRLLGGCLSLINPLIGTPYQPDFTGAVLVIEDIGE
                     DVYGIDRYLVQLRYAGILERLSGLIFGQFLDMESGEKTEPTLSLEEVLEKYTRDLNIP
                     ILGNFPYGHQDFKYTLPFGCRVRIEADNGTLRLLEPPVAAPAGPAA"
     gene            3951..4586
                     /gene="purN"
                     /locus_tag="PKM23_05910"
     CDS             3951..4586
                     /gene="purN"
                     /locus_tag="PKM23_05910"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNY91045.1"
                     /translation="MLTLCVFVSGRGSNLAALIEAIRLGRLDARIALVISNKAEAGAL
                     ALARANAITALHLSPAQFTSEEAYSSSLLGLLEEHGVGLIVLAGYLKKIPAAVIQHYR
                     HRILNIHPALLPAFGGKGMYGHHVHEAVLDYGCKVSGATVHIVDSDYDTGAPVLQQCV
                     PVLEDDTPERLAARVLETEHEILPRAVQLFAEGRIRIEGRRVTILAKGGRE"
     gene            4760..6310
                     /gene="purH"
                     /locus_tag="PKM23_05915"
     CDS             4760..6310
                     /gene="purH"
                     /locus_tag="PKM23_05915"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNY91046.1"
                     /translation="MRIGRALLSVYDKEGLLELARVLHGRGVEIISTGGTARYLQEAA
                     IPVRAVSEVTGFPEILGGRVKTLHPHIHGAILAKREDEEQMRQLTELGLTPIDLVVVN
                     LYPFEKTIARPDCTRADALENIDIGGPCMIRASAKNYPGVAVLTSPDQYGDFLHQFEE
                     LEGCTSLAMREQWAAAAFTRSSRYDAAIQAYLEAPAPAGETAFPQTLQLRLHKLQDLR
                     YGENPHQSAAFYAEEGGDAFGEQLHGKELSYNNILDIHSAVGLAHEFREPAVVIVKHN
                     NPCGVAIGTDLLEAYTKAVATDPASAFGGIVALNQPLTRSDLAAALAGTFLEVVVAPD
                     FSAEVLELLRSKKNLRLIRWPGFRIKPAMQDVRKVSGGFLIQNVDIEPVEPSTYRVVS
                     RRAPGEVELQALLFGWRVAKWVKSNAIIFAAPDRTLGIGAGQMSRVDSSRLAAMKAGH
                     AGLGLRGSAAASDAFFPFRDGLDVLAEAGATAVIEPGGSVRDDEVIAAADEHNIALVF
                     TGMRHFRH"
     gene            6382..7413
                     /locus_tag="PKM23_05920"
     CDS             6382..7413
                     /locus_tag="PKM23_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNY91047.1"
                     /translation="MSFGLGSFFSNDLAIDLGTANTLVYVRGRDVVLDEPSVVAIRKS
                     TQEIVAFGNQAREMVGRTPGEIITIRPLKDGVIADFELAEQMIRHFIRKAMPTRIPRP
                     RIAICVPTGITEVEKRAVRDSAEHAGAREVYLIEEPMAAAIGLGLPIDQPVGNMIIDI
                     GGGTCEIAVIAMYGIVNSVSIRIGGDEMNEAIVQYFKKSFNLLIGEITAEEIKCDLGS
                     AAPYDGKGTMTVKGRDLVAGIPKTVNISTKQVQEALSDTVDQLVDSVKMCLEQTPPEL
                     SADILDRGILMSGGGSMLKGLDERLRRETNLPVILAEEPLLAVVRGTGKVLEDITRFR
                     KVLTHIKRY"
     gene            7471..9654
                     /locus_tag="PKM23_05925"
     CDS             7471..9654
                     /locus_tag="PKM23_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNY91048.1"
                     /translation="MTIKHTSIMADVRRKLSAIPSDKRHKRFWGAILALALLFIGWLI
                     YLGFSLPKLDTLEDYNPQLASKVYSTDGVLIKEFFTERRFYVPMSQIPDVMIKSVLAT
                     EDARFYDHWGIRPMRFARAILFDVLTLSKQQGASTLTQQLARRLYLTPEKTVTRKIRE
                     WMTAIQIERTYTKPEILEMYLNHMSFAHGTYGVEAAAQYLFGKKAHELTLPECAVLTA
                     SLQRPAYWNPIRYPERALKRRNVVVLNRMRAEKFITEAQYRDAVAAPLGVKERTGSEL
                     EGIAPYFTEWVRQNLQKEFGWDLYKSGLNIYTTLDSRVQAAGEAAMRTYLPKMQEISN
                     RNLRKKEVLAKFLTPEQLKQDTVERWLTRKAFVDSVLNERAAVQIALVALDPRNGHIL
                     AMIGGRDFSESKYNRAVQAIRQPGSAFKPIVYTAAVDNGYMPCYEKLNQPVVVELGDG
                     TRWTPHNYDESIGGKTTLREALRRSLNLVTTRLVQEDIPPRQVVQYARHLGITTQLDA
                     VDALALGSCGVIPIELISAFGVFANHGVLVQPFGVMKVQDKYGNDLQKNSPQSKGVLR
                     EETAYIMADMLRTVAQAGTGAQAVSQYGFARPAGGKTGTTNDFTDAWYITFTPQMVVG
                     VWLGHDDPALTLGNKQTGTNAALPITATTIKNAHIALRLPEEWLSQPGGVVRLAICNE
                     TKLLASPACPDVVEELFDVRYQPTESCNLHVGGRGRDRDKRRIRY"
     gene            complement(9779..11410)
                     /locus_tag="PKM23_05930"
     CDS             complement(9779..11410)
                     /locus_tag="PKM23_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNY91049.1"
                     /translation="MNQPQWSASENYSYPLLIKNLLFAPAVYNPNQEIVYRGEKRYTY
                     QQFRERVRRLAGALVRMGIKQGDTVAVMDWDSHRYLECFYAVPMIGAVLHTINIRLSP
                     EQMVYTFSHAEDSALFINAEFLPLLDHIKGRIDTIKHFVLLNDSSTPPNHPFKFDNEY
                     EKLLAEAEPLQEFPDFDENLRATTFYTTGTTGMPKGVFFSHRQLVLHTLSGLVSLASN
                     RAHGSFHRDDVYMPLTPMFHVHAWGIPYMATMLGVKQVYPGKYAPDTILRLIDQEKVS
                     YSHCVPTIMHMLLKHPNSENVDLSGWKVIIGGAALPKALCLAALARGIDIYSGYGLSE
                     TCPILTISMLNAEELQQPPEEQTTLRCRTGRPFALVHLRVVNEQFRDVPDDDLTSGEV
                     VVRTPWLTPSYLKDNVNSEKLWAGGWLHTGDVAARDSRGSIRITDRLKDVIKVGGEWL
                     SSLELEDILISHPAVSEAAVIGQPNDRWGEVPLAFVVRKENETLTAKQLIAHVREFID
                     KGLVPGEAILLKVQFVGALEKTSVGKVNKQALRGK"
     gene            complement(11457..>11693)
                     /locus_tag="PKM23_05935"
     CDS             complement(11457..>11693)
                     /locus_tag="PKM23_05935"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY91050.1"
                     /translation="RFHLDQPDRVRLTIYNTLQQVVHQGPWHPHAAGAQELVWNGRNH
                     AGQLVATGLYFYRLETEHPLQEGVKMVGRFVYTP"
BASE COUNT         2261 a         3710 c         3558 g         2164 t
ORIGIN      
        1 ccccatgaag tcctactcgt catcgacgcc accaccggac agaacggcct ctcccaggcg
       61 cgccggttcg ccgaggcggt caaggtgacc ggtgtgatca tcaccaagct ggacggcacc
      121 gccaagggag gcatcgtctt ctccatcgcc cgcgaactgg gcatccccgt ccgctatgtc
      181 ggcctgggcg aaaaaatcga cgacctgcag agcttcgatc cccaaacctt tgtcgaggcc
      241 ctcttcgcgt gaaactgcac tatgtaagcc ttggctgccc aaagaacagc gtcgatctcg
      301 agaccatcct cggcggcctc accggcggca tggagctggt ggaagagccg gaaatcgccg
      361 atgtcgccct ggtcaatacc tgcgccttca tcaccagcgc caaacaggag gcgattgaaa
      421 cgattttgca gctggcggcg ttgaaggagg agaggcccgg cctcaagctg ctggtcagcg
      481 gctgcctgcc ccagcgctac cgcgccgagc tcgaggaact gctgccggag gtggaccgct
      541 ttttctttga tctcgatgcc ggcgtcactt cggccggaat cgccgcttat ctcggcgtgg
      601 ccgcccaccc ctgcgcgcga cgccaccgcc tgacaccgcg ccactatgcc tatctgcgca
      661 tcgcggaagg gtgcgacaac cgctgcagct actgcgccat ccccctcatc aagggacctg
      721 gccgcagccg ccctttggcg gagatccgga ccgaggcgga actgctggcg gccgacggca
      781 tgcaggaggt gatgatcgtg gcccaggaca gcacccgcta tggcctggac cttggccgtt
      841 ccgtccgcct gcatgacgtg ctcgccgcgc tgcaggaggt gtcccggctg cgctggatcc
      901 ggctgctcta cagccatccc gcgcactggt acgatgagct gatcgaggcc gtggcagccc
      961 tggacaaggt ggtcccctac atcgacctgc ccatccagca catttccgat cccctcctgc
     1021 gggcgatggg ccgcaaaagc agccgcgggc agatcgaggc gctgatcacg aggctgcgca
     1081 gccgcatccc cggcctggcc ctgcgcacct cgctgatcgt cggctttccg ggcgaggggg
     1141 agcgggaatt ccaggaattg tgcgatttcg tccgacagat ccgcttcgag cggctcgggg
     1201 ttttcagtta ctcccaggag gaggggacgc ccgcggaaac ctggccccag agcgtgagcg
     1261 aggaggagaa agaggcccgc ctgcaggaga tcatggccat ccaggcggag ctttccctgc
     1321 agaacaatcg cggattgatc ggccgtcggc tgcaggtcct ggtggatcag cccggagagg
     1381 agccgggcac ctgcctggct cgtacggtct gggatgcccc cgagatcgat aacagtgtca
     1441 tcgtcagcgg ggagcttgcg cccgggagtt ttgcaatggt gcggatcacc ggtgccgcac
     1501 cctacgatct ttacgccgaa ccggccgggg atgattaaaa aaggaaatga gttccgggca
     1561 atgcgtgttt taccttttag gtgaaaccgc ccgcagcggt cgccgaatcc ggaaggagct
     1621 ggagaatgcc atttatgcac aaagcaatat tcgccgcagt attgatgctg gcagttacgg
     1681 caacccgcat gcaggcccag gtagaattca gtaccatgca ggagcagagt ggacccgatt
     1741 tccatgtgga tctggtcaac accgcagtcg gaccagccag ccgactgagc cgcctcaatg
     1801 tctatctcga gatcgtctat gatgagctgc agttcgtcaa gaaggaggag aattttgaag
     1861 cgacctatga gatcagcgtc accatcatgg acaaggatga tgaccaggct gatggggaga
     1921 tctggaagga attggccacc gcaaccagct ttgacatgac caacgagcgc acacggctga
     1981 gccagagcca ccgccacttt gacctcgaac cgggccatta caaggtcaat atcgccgttc
     2041 aggataacga aacccaccgc ggctccaccc gcagcctctc catcgatctc cggaattacc
     2101 aggagagcac gatgcgcggc agcgatatcc tcctcgtcag cgaaatgcag cgcgattcga
     2161 ccggggcgat ctcgatccgc ccgcaggtga gcgacgcgac caagggtatt gtcgattcga
     2221 cccaggcttt cttcgaggtt tactgcgaga atcctcccca ggagctcacg gtgagctatg
     2281 aactcgccgg caagatcacc cacaagcgca tccgccgctc gttcacccat accctgaact
     2341 cctggcgcaa tcccgtctat ttcaagctgc accccgacag cctgccgcag gattcctatc
     2401 tgctcaccgt gcagatcgag tcggcgggcg gcaagatcaa gcgtgaaaag ccggtctaca
     2461 tccgctggag cgccctgccg gcgacggcgt cggacctcaa gaccgccgtc gaacagctgc
     2521 gcctggtcgc ctccaaggat gagtggaagc ggctgaagaa agccaagggg gatgccctgc
     2581 tcaccgagtt caaggccttc tggaagcgcc atgatcccag ccccggcacc gatgccaacg
     2641 agactatgga tgccttttat tctcgggtcg aattcgccaa ccagcgcttc tcggtcatgc
     2701 agatgcctgg ctggcgtacc gatatggggg taatctttat cattctgggc tcacccgaca
     2761 acgtggaacg cgatgtctat cctcgcgata tgaagcccta cgaaatctgg acctattatg
     2821 agttcaaccg ggattttctc tttctcgatt acaccggctt cggcgactac cgcctgcaaa
     2881 cccctatgtc catttacgaa tttcaacggc tgctgcataa ctgacaggaa tccccatgga
     2941 gcttcgcatt ccccctgccc tggtccccgg cgaccgcatc ggcgtcatct ccccggcgag
     3001 cccgcccaaa cccgaaaagc atgatcagta ccagaacggt ctggcctacc tgcgtggatt
     3061 gggatacaag atcagcgagg gcaagcacgt ccttgaccaa tacggctatc tggccggcac
     3121 tgacgaaagc cgcgcggccg acctcaatgc gatgttccgc aacccccgga tcaaggcgat
     3181 catctgctcg cgcggaggct acggaacccc gcggctgctc gatctgatcg attatgaggc
     3241 ggtccgcaaa catccgaaaa ttctcgtcgg ctacagcgac atcacctcat tgcagctcgc
     3301 cctcttcgcc cgcactggtc tggtcacctt ctccggcccc atggtcgctg tggagatggg
     3361 caaggggatt ctccccttca ccgagaagca cttctgggcg atggtgggca agagcacgcg
     3421 caagctcctc aaggccggtg cgggggagta tgagccgcgc atctatcacg gcggcgtcgg
     3481 cgagggacgc ctgctcgggg gctgcctctc cctgatcaat cccctcatcg gcacccccta
     3541 ccagcccgat ttcaccggcg ccgtcctggt gatcgaggat atcggcgagg atgtctacgg
     3601 catcgaccgc tacctggtcc agctgcgcta cgccggcatc ctcgagaggc tcagcggcct
     3661 tatctttggt cagttcctgg atatggagag cggcgagaaa accgaaccca ctctgagcct
     3721 cgaagaggtc ctggaaaaat acacccgcga tctcaacatc cccatcctgg gcaatttccc
     3781 ctacggtcac caggatttca agtataccct gcccttcggc tgccgggtcc gcatcgaggc
     3841 ggacaatgga accctgcgcc tgctcgagcc gccggtggct gcccccgcgg ggccggcggc
     3901 ctgatgtcga gggcagacgg gctgcagcgc tgttgagagg agaaaccgcc ttgctcacac
     3961 tctgtgtctt cgtctccgga cggggctcca acctggcggc cctgatcgag gcgatccgct
     4021 tggggcgcct cgatgcgcgc atcgccctgg tgatcagcaa caaggccgag gccggggccc
     4081 tggccctggc acgagcgaac gccataacgg ccctccacct ttccccggct cagttcactt
     4141 ccgaggaggc ctacagcagc agcctgctcg gcttgctgga agagcacggc gtcggcctga
     4201 tcgtcctcgc cggctatctc aaaaagatcc ctgctgcggt catccagcac taccgtcacc
     4261 gcatcctcaa tatccatccc gccctgctgc ccgcctttgg cggcaagggg atgtacggcc
     4321 atcacgtgca tgaggcggtc ctggattacg gctgcaaggt gagcggggcg accgttcata
     4381 tcgtggacag cgattacgat accggcgccc cggtgctgca gcagtgcgtg ccggtgctgg
     4441 aggatgatac gccggagcgt ctggcggcca gggtgctcga gaccgagcac gagattttgc
     4501 cccgcgccgt gcagctcttc gccgagggga ggatccggat cgagggccgc cgggtcacaa
     4561 ttctggcgaa ggggggaagg gaatgacgat tcagggcgga aacacccgct gcagcaccga
     4621 tttgttgaaa aaaaacggca aaatagcggg cccgctgcag caaattcttt gatttttgct
     4681 atttttttga tatacttatt ttcatgcttt atatgaacaa gtcctgaaat ggtgaaggga
     4741 ggactttttg ccggatcaca tcagaatagg gcgcgccctt ctcagcgtct atgacaagga
     4801 gggtctgctg gagctggccc gggtcctgca cggacgcggc gtggagatca tttcgaccgg
     4861 tggaaccgcg cgttatctgc aggaggccgc catcccggtc cgtgccgtct cggaggtcac
     4921 cggtttcccc gaaatcctcg gcggcagggt caagaccctc catccccata tacacggcgc
     4981 catcctcgcc aaacgtgagg atgaggagca gatgcgccag ctgacggaac tggggctgac
     5041 cccgatcgac ctggtggtgg tcaatctcta tcccttcgaa aagaccatcg ccagaccgga
     5101 ctgcacccgc gcagacgccc tggagaatat cgatatcggc ggcccttgca tgatccgtgc
     5161 ctccgccaaa aattatccgg gcgtggcggt gctgaccagc ccggaccagt atggtgattt
     5221 tctgcaccag ttcgaagagc tggagggctg cacgagcctg gccatgcgcg agcaatgggc
     5281 ggcggccgcc ttcacccgta gtagccgcta cgatgccgcc attcaggcct atctcgaggc
     5341 cccggccccc gccggcgaaa ccgcctttcc ccagaccctg cagctccgcc tgcacaagct
     5401 tcaggatctg cgctacggcg agaatcccca ccagtcggcc gccttttacg cggaggaggg
     5461 aggcgacgct ttcggcgagc agctccacgg caaggagctc tcgtacaaca atatcctcga
     5521 tatccattcc gccgtcggcc tggcccatga gttcagggaa ccggcggtgg tgatcgtcaa
     5581 gcacaacaac ccctgcggag tggccatcgg gaccgacctg cttgaggcct acaccaaagc
     5641 ggtggcgacc gatccagcct ccgcctttgg cgggatcgtc gccctcaacc agcccctgac
     5701 ccggagcgac ctcgccgctg cgctggccgg gacctttctt gaggtggtgg tggctcccga
     5761 cttttccgcc gaggtgctcg aactgctgcg cagcaagaaa aatttgcgcc tgatccgctg
     5821 gcccggcttc cgcattaaac ccgccatgca ggatgtgcgc aaggtcagcg ggggctttct
     5881 catccagaat gtcgacatcg aaccggtgga gccctccacc taccgggtgg tcagccgccg
     5941 ggcgccgggc gaagtggagc tgcaggccct gctctttggc tggcgcgtgg cgaaatgggt
     6001 caagagcaat gccatcatct tcgccgcccc ggaccgcacc ctgggcatcg gcgccggcca
     6061 gatgtcgcgg gtcgattcct cgcgcctggc cgcgatgaag gccggtcatg ccggactggg
     6121 gctgcgcggc agcgccgccg cctccgacgc ttttttcccc tttcgggacg gcctcgatgt
     6181 cctcgccgag gcgggggcca cagccgtcat cgagccgggg ggctccgtgc gcgatgacga
     6241 ggtcatcgcc gcggccgacg aacacaatat cgccctggtt ttcacgggca tgcgccattt
     6301 ccgccactga gcggagccgg ccgtactgat tttttattac agcgggattg cattctcact
     6361 atttgaacaa ggttgggcaa catgagcttc ggtttgggtt cctttttctc caatgacctg
     6421 gctatcgatc tcggcaccgc caataccctg gtctatgtac gcggccggga tgtggttctc
     6481 gacgagccat ccgtcgtcgc catccgcaag agcacccagg agatcgtcgc cttcggcaac
     6541 caggcgcgcg agatggtcgg ccgcaccccc ggcgagatca tcaccatccg tcccctcaag
     6601 gacggcgtca tcgccgattt cgagctggct gaacagatga tccgccactt catccgcaag
     6661 gcaatgccga cgcgcatccc gcgcccgcgc atcgccatct gtgtccccac cggcatcacc
     6721 gaggtggaaa agcgggcggt gcgcgattcg gcagagcacg ccggcgcgcg cgaggtttat
     6781 ctcatcgaag agcccatggc ggccgccatc ggcctcggcc tccccatcga tcagccggtg
     6841 ggcaacatga tcatcgatat cggcgggggc acctgcgaga tcgcggtcat cgccatgtac
     6901 ggcatcgtca actcggtctc catccgcatc ggcggggatg agatgaacga agccatcgtt
     6961 cagtatttca agaaatcctt caacctcctc atcggtgaga tcaccgcgga ggagatcaag
     7021 tgcgatctgg gctccgctgc cccctacgac ggcaagggca ccatgaccgt aaagggacgc
     7081 gacctggtcg ccggcatccc caagaccgtg aacatcagca ccaaacaggt gcaggaggcc
     7141 ctctccgaca ccgtcgacca gctcgtcgat tcggtcaaga tgtgccttga gcagaccccg
     7201 cccgagctct ccgcggatat tctcgatcgc ggcatcctca tgagcggcgg cggctcgatg
     7261 ctcaagggac tcgatgagcg gctgcgccgc gagaccaacc tgccggtcat cctggccgag
     7321 gagcccctcc tcgccgtggt gcgcgggacg ggcaaggtcc tggaggatat cacccgtttc
     7381 cgcaaggtat tgacgcacat taaaagatat taggaacaaa ccggacgccc tgccctgcgg
     7441 ggcgttcttt ttattagggt tagcctggat atgacgatca agcatacctc catcatggcc
     7501 gacgtgaggc gcaagctctc cgccatccca tccgacaagc ggcacaagcg cttctggggc
     7561 gcgatcctcg ccctggccct gctctttatc ggttggctca tttacctcgg cttcagcctg
     7621 cccaagctgg acaccctgga agattataac ccccagctcg cttccaaggt ctacagcacc
     7681 gacggtgtgc tcatcaagga gttcttcacc gaacgccgct tttacgtgcc gatgtcccag
     7741 atccccgatg tcatgatcaa gtcggttctg gccacggagg atgcccgttt ttacgaccac
     7801 tggggcatcc ggccgatgcg cttcgcccgc gccatcctct tcgacgtcct cactctgagc
     7861 aagcagcagg gcgcgagcac tctcacccag cagcttgccc gacggctcta tctgaccccc
     7921 gagaagaccg tgacgcgcaa gatccgcgag tggatgaccg ccatccagat cgagcgtacc
     7981 tataccaagc ctgaaatcct cgagatgtac ctcaaccaca tgtccttcgc ccacggcacc
     8041 tacggggtcg aggcggcggc ccagtacctc ttcggcaaaa aagcgcacga actgaccctg
     8101 cccgaatgcg ccgtactgac cgccagcctg cagcggccgg cctactggaa cccgatccgc
     8161 tacccggaac gcgccctcaa gcggcgcaat gtcgtggtgc taaatcggat gcgcgccgaa
     8221 aagttcatca ccgaggcgca gtaccgggat gccgttgccg ctccgctcgg ggtgaaggag
     8281 cgcacgggat cggaactgga ggggatcgcc ccctatttca ctgaatgggt gcggcagaat
     8341 ctgcagaagg agttcggctg ggatctttac aagagcggac tgaacatcta taccaccctc
     8401 gattcgcggg tgcaggcggc cggcgaggca gcgatgcgca cctatctgcc caagatgcag
     8461 gagatctcca accgcaacct gcgcaagaag gaggtgctcg ccaaattcct caccccggaa
     8521 cagctcaagc aggatacggt ggagcgctgg ctgacgcgca aggcctttgt cgattcggtg
     8581 ctcaacgaga gggccgccgt ccagatcgcc ctggtggcgc tcgacccgcg caacggccat
     8641 attctggcga tgatcggggg acgggatttc agcgagagca agtacaaccg ggcggtccag
     8701 gccatccgcc agcccggctc cgccttcaag cccatcgtct atacggcagc agtggataac
     8761 ggctacatgc cctgctacga aaagctaaat caaccggtgg tggtcgagct gggcgacggc
     8821 acgcgctgga ccccccacaa ttacgatgaa tcgatcggcg gcaagaccac cctgcgcgaa
     8881 gccctgcgcc gctcgctcaa tctggtcact acccgcctgg tgcaggagga tatccccccc
     8941 cgccaggtgg tgcagtatgc gcgccatctc ggcatcacca cccaactcga tgcggtggac
     9001 gccctcgccc tcggctcttg cggcgtcatt ccgatcgagc tcatctccgc ctttggcgtc
     9061 ttcgccaacc acggcgtgct cgtccagccc tttggggtca tgaaggtgca ggacaaatac
     9121 ggcaatgacc tgcagaaaaa cagtccgcag agcaagggcg tgctgcgcga ggagaccgcc
     9181 tacatcatgg ccgatatgct gcggacggtg gcccaggcgg ggaccggcgc ccaggcggtc
     9241 tcgcagtacg gctttgcccg tcccgccggt ggcaagaccg gcaccaccaa tgatttcacc
     9301 gacgcctggt acatcacctt caccccgcaa atggtggtgg gggtctggct cggccacgat
     9361 gatccggccc tgaccctcgg caacaaacag accggcacca atgccgccct gccgatcacg
     9421 gccaccacga tcaaaaacgc ccacatcgcc ctgcggctgc ccgaggagtg gctgagccag
     9481 ccgggcgggg tggtccggct cgccatctgc aacgagacca agctcctcgc cagccccgcc
     9541 tgccccgatg tcgtggaaga actcttcgat gtccgctacc agccgaccga aagctgcaat
     9601 ctgcacgtcg gaggcagggg gcgcgaccgc gacaaacggc gcatccgtta ctgagtcaca
     9661 gccaccggcc gggatgtaaa aagcccgccc cgctatctgc catagggcag gagaggggcg
     9721 ggctttatat tttgtgaaga ggtttacaac ggcataagcc ggacgatggc tgcgatcact
     9781 attttccccg cagggcctgt ttgttgacct tgcctacgct ggtcttctcc agggcgccga
     9841 cgaactgcac cttgaggagg atggcttcgc cggggaccag ccccttgtcg atgaattcgc
     9901 gcacatgggc gatcagctgc ttggcggtca gggtttcgtt ttccttgcgc acgacgaagg
     9961 cgaggggcac ctcgccccag cggtcattgg gctggccgat gacggcggcc tcggagacgg
    10021 ccggatggct gatgaggata tcctcgagtt ccagcgaaga gagccattcc ccgcccacct
    10081 tgatcacatc cttgaggcgg tcggtgatgc ggatgctgcc acggctgtcg cgcgcggcga
    10141 cgtcgccggt gtgcagccag cccccggccc agagcttttc ggaattgacg ttgtccttga
    10201 gataactcgg ggtcagccag ggtgtcctca ccaccacctc gcccgaggtg aggtcgtcgt
    10261 cagggacgtc cctgaactgc tcgttgacca cgcgcaggtg gaccagggcg aaggggcgcc
    10321 cggtgcggca ccgcagggtg gtctgttctt ccggcggctg ctggagctcc tccgcattga
    10381 gcatcgagat cgtcaggatc gggcaggtct ccgagaggcc atagcccgag tagatgtcga
    10441 tgccgcgcgc cagggcggcc aggcagaggg ccttgggaag ggctgcaccg ccgatgatga
    10501 ctttccagcc ggagagatcg acgttctcgc tgttgggatg cttgaggagc atgtgcatga
    10561 tggtcggcac gcagtgggag tagctcacct tctcctggtc gatcagcctg aggatggtgt
    10621 cgggggcgta cttgccggga tagacctgtt tgaccccgag catggtcgcc atgtagggaa
    10681 tgccccaggc gtggacatgg aacatcgggg tcaggggcat gtagacatcg tcacgatgga
    10741 aggaaccgtg ggcccggttg gaggcgaggg agaccagccc gctcagggta tggaggacga
    10801 gctggcggtg ggagaaaaag actcctttgg gcatcccggt ggtaccggtg gtataaaaag
    10861 tggtcgcgcg cagattttcg tcgaaatcgg ggaattcctg caggggctcc gcctcggcga
    10921 ggagcttttc gtactcgttg tcgaacttga aggggtgatt ggggggtgtg ctgctgtcgt
    10981 tgagcagcac aaaatgcttg atcgtgtcga tgcgcccctt gatatggtcg aggaggggga
    11041 ggaattcggc attgatgaag agggcgctgt cctcggcatg gctgaaggta tagaccatct
    11101 gctcgggcga gaggcggata ttgatggtgt ggagcacggc gccgatcatg ggcacggcgt
    11161 agaaacattc gaggtagcgg tggctgtccc agtccatcac ggccaccgta tcgccctgct
    11221 tgatccccat cctgaccagg gccccggcca gacgtcggac cctttcgcgg aactgctggt
    11281 aggtgtagcg tttctccccc cggtaaacga tctcctggtt gggattgtag acggcgggtg
    11341 cgaagagcag atttttgatc aggagaggat aactgtaatt ctcactggcg ctccattggg
    11401 gctgattcat gcttgacctc gcaaaaaggg ggaccggttg gcttgttaat attaatttac
    11461 ggtgtatata cgaaacgtcc aaccattttt acgccctctt gcagcggatg ttcggtttcc
    11521 agacgataga agtagagtcc ggtggcaacc agctggccgg cgtggttgcg gccattccag
    11581 accagctcct gcgccccggc cgcatgggga tgccaggggc cctggtggac gacctgctgc
    11641 agggtgttgt agatggtcag gcgcacgcgg tccggctggt caagatggaa acg
//
LOCUS       DAOPWU010000080        11690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5620_length_11690_cov_5.076579, whole genome shotgun sequence.
ACCESSION   DAOPWU010000080 DAOPWU010000000
VERSION     DAOPWU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11690
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5620_length_11690_cov_5.076579"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKM23_05940"
     CDS             <1..339
                     /locus_tag="PKM23_05940"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNY91051.1"
                     /translation="PTYSLVQSYDDRAFSCAAVIQRNRLEGMFKWEGYNRGLLTGIYL
                     SGDVRKGDVIVTSGTNSIYVPGLKLGVVSSIDDAEAGMYRRIYIETKVDITRLREVYI
                     IQGSAGERPR"
     gene            336..845
                     /gene="mreD"
                     /locus_tag="PKM23_05945"
     CDS             336..845
                     /gene="mreD"
                     /locus_tag="PKM23_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNY91052.1"
                     /translation="MNFIYYLIMVAVALGLQHGVSDLVAIAGVNPDFLLIAVCAIALY
                     AGRGQGTIWGAAVGFIEDSLTGGLIGSHAFVLSLVAFIAGSMMSYRSLQSAYSLTAGL
                     IVGLLALVHNLFLYLLNVHGAAGWLTGLLMAVFLPAVYTLFWALIIFAVVPESIWEKI
                     YKTEPSTLF"
     gene            864..2654
                     /gene="mrdA"
                     /locus_tag="PKM23_05950"
     CDS             864..2654
                     /gene="mrdA"
                     /locus_tag="PKM23_05950"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNY91053.1"
                     /translation="MLSPDRTTRIIQLIITGAFIALAGQLFNLQILQREKYAVQSEKN
                     RSRRLRLEPPRGIIYDRSGTPLVENRPSYVLSVIPIEVRNNDKSLAFLAGLMQETPDE
                     IKRRLARADNPFLPFKLRRDIDYGILVQLEEHKLEYPGVTYEIESRRTYPAGLKSPHL
                     FGYIGEISKSELQRRQEEDMLPGDLVGKKGFEAYYDRELRGQVGYKVVIVDAVGRMAS
                     DQTGSSEQAAKRGRDFYLALDANLQALADSLFADKRGGLVMVDTRDGGVLALCSKPDF
                     DPEIFTGTLTNEEWQRLVNDPAKPLYDRMIQSSYPPGSTLKMVAATAALEERKANQET
                     YFSCGGGVSFGDRVFSCWNKGGHGSLNLIDALKVSCNVYFYRLSLRLTVDEWALYASR
                     YGLGKRTGIDLPAEEKGTLPDRAYLDRAFGKNGWTNGMMLNLGIGQGDLLVTPVQMVQ
                     YTTIIANKGRYYPLHLVHKIYDPRTQSFFRQKLAQRTVEGISDATYDIIREGMYRVVN
                     SPGGTGHNSYLDDVVVAGKTGTAQNPHGEPHAWYVGFAPFDHPEVAICVLVENGGGGG
                     ATSGPIAGRLLRRYFDLQKKSPALAAAPKP"
     gene            2665..3888
                     /gene="rodA"
                     /locus_tag="PKM23_05955"
     CDS             2665..3888
                     /gene="rodA"
                     /locus_tag="PKM23_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNY91054.1"
                     /translation="MLDEIRNTRVDKVILICVLLFSLFSLLAVYSATHGSRVAAIHDN
                     FYKQIIWVVAGLLLFFALTYVPLHIIFSLAYPLYGILLVMILGIDLVGSISGGAQRWF
                     EVGGIKVQPSEFMKPIVVLTLARFLSPEHANPNRLKNLLIAFAIVLAPFVLVLKQPDL
                     GTSLVYLSMVIPVLYWRGLSPFTIFVVVAPLFAFLASFNYYAFSAVILLISVVLYLSR
                     RGKRVAWTVFVMNVFVGTLSPLVWSGLHGYQQQRILTFLGLVEDPRGVGYQIIQSMVA
                     IGSGGIFGKGLLHGTQTQLRFLPAQHTDFIFSVLAEEWGFIGSLAVLALFFVFIMRAI
                     KIAASSSYRFASLAAMGMATIFAFQAIVNLGMTMGIMPVTGVPLPFISYGGSAMLTNM
                     TMAALLSNAITQRYF"
     gene            3982..4803
                     /gene="lgt"
                     /locus_tag="PKM23_05960"
     CDS             3982..4803
                     /gene="lgt"
                     /locus_tag="PKM23_05960"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNY91055.1"
                     /translation="MHPVLFKIGAFEMRSYGFALAISFLLGIYLAVRRGRKRGIDPEK
                     IMDLSVVIILASIIGARFMYVIFHLDEFAGHWTDTFNPFQSNGQIGIAGLTMLGGVVL
                     AVVCSLLYLRAKKQPILRIADTIIPLFFLGEAITRIGCYLNGCCYGIPCDGPLCVVFP
                     PDSPAGAMYQGIHIHPAQLYSSAYALLVFIIMLVIDRKPKYDGFLLYLFFIFYGVGRF
                     IIDLFRYYESSMILLPLGAKGLSMNQGISLAFIVIGIFLLVRQERRERVGAAPGK"
     gene            4959..5750
                     /locus_tag="PKM23_05965"
     CDS             4959..5750
                     /locus_tag="PKM23_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY91056.1"
                     /translation="MKREPIVRILGGMALAAMVVVWSGCSSSRQVSLATEQAALSGAE
                     EKATTDTQTANKSSDEDEVMRLLGLNKSESAVEAAPQPTAAGLEEDRKRLEEALKERD
                     IEIANLKAELAERDRQLNAAESAEPPMEIKPVRTPVSGSGTFQERYDRARALYESRRY
                     KEAIQEFAALLSTDSSHKLADNCQYWIGECYYGMRAYNQAIVEFEKIFTFNDSDKNDD
                     AQLKLGLCYTRIGNVEKAKAEFQKLVDTYPESEFISRAQYYLQTL"
     gene            5935..7428
                     /gene="glgA"
                     /locus_tag="PKM23_05970"
     CDS             5935..7428
                     /gene="glgA"
                     /locus_tag="PKM23_05970"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNY91057.1"
                     /translation="MKNRLRVLYLSTEVAPFAKTGGLADVASALPKALFEQGHDVRVM
                     MPKYGNISERRYTLREVIRLKDIPIGVGGQEHVVSAKSAFLPETKVQVYFLDYKPFFE
                     RSDYYVDSATGKDFPDNAQRFFLFCKAVLETIKLLHWEPHIIHCNDWQTALIPWLLKN
                     HYQDDPFFAKAITVLSIHNLAFQGIFPQSVLPELGLAAELGQAGTDLEFYGKVSFLKA
                     GIRSADLITTVSPTYSAEIQSDPELGCGLQGVLLERKEDLCGILNGVDYGIWNPEKDA
                     LIPVKYSAATLKDKEKNKKALAKEAGFPFDPAVPVVGMISRLTSQKGFDLVAAAIDDL
                     LKLKIQLVILGTGDPVYHKQLEKLAKAHPKQVALFLKFDDTLAHLIEAGSDMFLMPSL
                     YEPCGLNQMYSLKYGTIPIVRKTGGLADTIIDADADPTNGNGFVFAEYKAKAMVDAVR
                     RAVAAFRDEKGWQKLIKRCMKQDFSWQLAADKYIRLYLKLETAKKKK"
     gene            7841..8569
                     /locus_tag="PKM23_05975"
     CDS             7841..8569
                     /locus_tag="PKM23_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY91058.1"
                     /translation="MDGIEITVDRVGALRDIGLFRVRGYVDTTTSPELQRMLTEIIHD
                     GVTQIIIDLGAVNYVSSAGWGVFVGEIRGLREQGGDLKIIQMIPEVNEVFEMLEFNRI
                     LTCYDSLEEAIDDYDFCQGVDLRASLAEGAAAGKAVPAWETDRVVENPADYIPPANKE
                     SRTNRNITIARGRGIDDVDLPLNEKIKKLVTENPLMTPWGIKRQLNSPRFGYTRIGFF
                     KLRGILKRLGLETRAKRYRFYRSR"
     gene            8661..9368
                     /locus_tag="PKM23_05980"
     CDS             8661..9368
                     /locus_tag="PKM23_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNY91059.1"
                     /translation="MKRPLFFVYGYMAALLLGAVQGNAQTASPEFSPEALRVTPGDAI
                     HLYIYENLFPSEKGKFVSNYHDKEFVVDGLGQITLGPLGRVQIAGLKAEEIAQVLQEK
                     FKPFAKDPFIIVVPLIRLELKGGFSQPGLYRFNPNISFWEVIKEVGGLHSLSSYEDMF
                     ILRNGQALYRGFGDAFYRGQSLYELGLQSGDEIVAPRVNRLSFDTIIRYIQFAMSLFT
                     FYFVLINYNKNDNSNSL"
     gene            9384..>11690
                     /locus_tag="PKM23_05985"
     CDS             9384..>11690
                     /locus_tag="PKM23_05985"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNY91060.1"
                     /translation="MDDLDLLMPAGEQQSAPMDFGKFFNGFWKRKWIILLLTLIGAAW
                     FFWDSKNEKPVYSTFATIKTRSFDSDAESILSRDRQAELRSRSFAERVAARMGLALQM
                     GTKAGPLGDVFSEFHTTTSPTPGKYKIQVDDNGTFSIFSVDYPEETLLDSMSVWDVIS
                     REYTYEGFTFRLNPKWVEHSQFVTFKIRPFERAVAELNTMVTAEVSKNGNLMKLTIKG
                     EDRDHLADQLNQIADIYVQETLMLRDRDANSYRKRLEGSLEVAAEKVRAADADLRNFY
                     SKYPLSLDAAKKTILDEMQRNSYTLREIPQQRQQLTQLLEKLGEPVVGSDPEQYRRLI
                     VRQLANFSAMRDEPALALLRDQLDSQEKQLQDLMRDYSNDYAPLVSLRTSIRETQDNI
                     IAFASNYRNTLAQKESETRIKMADIQSQQRMLPEDEYRLMELERNKKVSEDHYTALYT
                     KLQKLEIADGGEGEDIAVLDRAIKPTVPINPSQSTKIFIGAALGLVAGLALSLGIDLA
                     DRHIRTPGDVERHLNLKVLGAIPNVDFKDIPEYHDHEKAKQIDRQLVTHDYSPTPIGE
                     AYRSLRTQIIFSRTHERPHSIAITSVAPEEGKSFTASNLAIIFAQQRTNTLLVDADLR
                     RGVLHNTFQMHREPGLANYLSSSVTLSSIVQQTHVPNLSFISCGVLLPNPSELLGSPL
                     MRRFLDEASRKFDIVLFDTPPLDAATDAVVLGTQVNAVVLVVRAGKTKIGKAKEKMEI
                     FHTVPVKLVGAVLNSSDTQLVQNYSYYHY"
BASE COUNT         2294 a         3640 c         3307 g         2449 t
ORIGIN      
        1 cccacctact cgctggtgca gtcctacgat gaccgcgcct tcagctgtgc ggcggtgatc
       61 cagcgcaacc gcctcgaggg gatgttcaag tgggagggtt acaaccgcgg ccttttgacc
      121 gggatctatc tgagcgggga tgtgcgcaag ggagatgtca ttgtcacctc gggcaccaac
      181 tccatctacg tgccggggct gaagctgggg gtggtctcct cgattgacga tgccgaagcg
      241 gggatgtacc gcaggattta catcgaaacc aaggtggaca tcacccggct gcgggaggtc
      301 tatatcattc agggttccgc cggggagagg ccgagatgaa ttttatctac tacctgatca
      361 tggtggcggt ggcgcttggc ctgcagcatg gcgtcagcga cctggtcgcg atcgcggggg
      421 tgaatcccga tttcctcctc atcgccgtct gcgcgatcgc gctctacgcc gggcgcgggc
      481 aggggacgat ctggggggcg gcggtcggtt tcatcgagga ttcgctgacc ggcggcctca
      541 tcggcagcca tgcctttgtc ctcagcctgg tcgcctttat cgccggctcg atgatgagct
      601 accgctcact ccagagcgcc tacagcctca ccgccggact catcgtcggc ttgctcgccc
      661 tcgtccacaa tctttttctc tacctgctca atgtgcacgg cgcggcgggc tggctgaccg
      721 gtctgctcat ggccgtgttt ctgcctgcgg tctataccct gttctgggcc ttgatcatct
      781 tcgcggtcgt gccagaatcc atctgggaaa agatctacaa aaccgagcct tcaaccttgt
      841 tttaaccggt ccgatgtcaa gccatgctct cccctgaccg caccacccgc atcatccagc
      901 tcatcatcac cggggccttt atcgccctgg cggggcagct tttcaatctg cagatcctgc
      961 agcgggaaaa gtatgcggtc cagtcggaga aaaaccgttc ccgccggctg cgcctcgagc
     1021 cgccgcgcgg catcatctac gaccgcagcg gcaccccgct ggtggagaac cggcccagtt
     1081 atgttctcag cgtcatcccc atcgaggtgc gcaacaacga caagtcgctt gcctttctcg
     1141 ccgggctgat gcaggaaaca cccgatgaaa tcaaacgtcg gttggcgcgt gcggacaatc
     1201 cctttctccc cttcaaactc cgccgggaca ttgactatgg catactggtg cagctcgagg
     1261 agcacaagct cgagtatccg ggtgtcacct acgagatcga atcgcggcgc acctatccgg
     1321 ccggactaaa gtcgccccat ctcttcggtt atatcgggga gatctccaaa agtgagctgc
     1381 agcggcggca ggaggaggac atgctgccgg gggatctggt gggcaagaag ggttttgagg
     1441 cctactatga ccgggagctg cgcggccagg tcggctacaa ggtggtcatc gtcgatgcag
     1501 tcgggcgcat ggccagcgat cagaccggtt cgagcgaaca ggccgccaag cggggcaggg
     1561 atttctatct cgccctggat gcgaatttgc aggccctggc cgactcgctc ttcgctgaca
     1621 agcggggcgg gctggtgatg gtcgacaccc gcgacggggg ggtgctggcg ctctgcagca
     1681 agcccgattt cgaccccgag attttcaccg gcaccctcac caacgaggag tggcagcggc
     1741 tggtcaacga tccggccaaa cccctctatg accggatgat tcagagttca tatcccccgg
     1801 gctcaaccct caagatggtc gccgcgaccg ctgcactgga ggagcgcaag gccaaccagg
     1861 agacctattt ttcctgcgga gggggcgtca gcttcggcga ccgcgtcttc tcctgctgga
     1921 acaagggggg ccacggctct ctcaacctga tcgacgccct caaggtctcg tgcaatgtct
     1981 atttttaccg gttgagcttg cgcctcaccg tcgatgaatg ggcgctctat gccagccgtt
     2041 acggcctggg caaacggacc gggatcgatc tgccggccga ggagaaagga accctgcccg
     2101 accgcgccta tctcgaccgc gccttcggca aaaacggctg gaccaacggc atgatgctca
     2161 acctcggcat tggccagggc gatctgctcg tcacccccgt gcagatggtt cagtacacga
     2221 ccatcatcgc caacaagggg cgctactacc cccttcatct ggtccacaag atctacgatc
     2281 cacgcaccca gagctttttc aggcaaaagc tcgctcaacg cacagtcgag gggatttcgg
     2341 acgccaccta tgatatcatc cgggaaggaa tgtaccgggt ggtcaacagt ccgggcggga
     2401 ccgggcataa cagttatctg gacgatgtcg tggtagccgg caagaccggc actgcgcaga
     2461 atccccacgg tgagccgcac gcctggtatg tcggcttcgc tccctttgac catcccgagg
     2521 tggcgatctg cgttctggtc gagaacggcg gcggcggcgg ggcgacctcc gggccgatcg
     2581 ccgggcggct gctgcgccgc tatttcgatc tgcaaaaaaa gtcgccagcg ctggctgcgg
     2641 cgcccaaacc ctagagagtg caccatgctc gatgagatcc gcaatacccg ggtggacaag
     2701 gtcatcctga tctgcgtcct gcttttttcc ctcttcagtc tgctggcggt ttacagcgcg
     2761 acccatggca gcagggtggc ggcgatccat gacaattttt acaagcagat catctgggtt
     2821 gtggccggtt tgctgctctt cttcgccctc acctatgtgc cgctgcatat catcttctcg
     2881 ctcgcctatc ccctttacgg catcctgctg gtgatgattc tgggcatcga tctggtgggt
     2941 tcgatcagcg gcggggcgca gcgctggttc gaggtgggag ggatcaaggt tcagccctca
     3001 gagttcatga agccgatagt ggtgctgacc ctggcccgct ttctttcgcc tgagcatgcc
     3061 aatcccaacc ggctgaagaa cctgctcatt gccttcgcca tcgtcctcgc cccctttgtg
     3121 ctggtgctca aacagcccga tctcggcacc agtctggtct atctttcgat ggtcatcccg
     3181 gtgctctact ggcgcggcct cagtcccttc accatctttg tcgtggttgc gcccctcttt
     3241 gcctttctgg cctcgttcaa ttattacgcc ttcagcgccg tcattctgct catcagcgtg
     3301 gtcctctatc tctcccggcg cggcaagcgg gtggcctgga cggtttttgt gatgaatgtc
     3361 ttcgtcggca ccctctcccc cctggtgtgg agcggcctgc atggctatca gcagcagcgc
     3421 atcctcacct ttctcgggct ggtcgaggat ccgcgcggag tgggctatca gatcatccag
     3481 tcgatggtgg cgatcggctc cgggggtatt ttcggcaagg ggctgctgca cggcacccag
     3541 acccagctgc gttttctgcc ggcgcagcat accgatttca tcttttccgt gctcgctgag
     3601 gagtggggct ttatcggttc gctggccgtc ctcgccctct tttttgtttt catcatgcgc
     3661 gccatcaaga tcgccgcttc cagcagctac cgtttcgcca gcctggccgc gatgggtatg
     3721 gcgaccattt tcgcttttca ggcgatcgtc aatctcggca tgaccatggg aatcatgccg
     3781 gtgacggggg tcccgctccc ctttatcagc tacggggggt cggcgatgtt gaccaatatg
     3841 accatggcgg ccctgctttc gaacgccatc acccagcgtt atttctgatt ccggctgaaa
     3901 catcggcggc gggactgtag cctgcactcc ggcgttctcc ctcggaacca ctgctgatca
     3961 ctctcttgcg gaggaatttt catgcacccc gtgctcttta aaatcggcgc cttcgagatg
     4021 cgttcctacg gattcgccct cgccatctcc tttctcctcg gcatctatct ggcggtgcgg
     4081 cgcggccgca agcgcgggat cgatcccgag aagatcatgg atctttcggt ggtgatcatc
     4141 ctcgcctcga tcatcggggc gcgctttatg tatgtcatct ttcacctcga cgagttcgcc
     4201 ggccactgga ccgacacctt caatcccttt cagagcaacg gccagatcgg catcgccggc
     4261 ctgaccatgc tcggcggggt ggtccttgcc gtcgtctgct ccctgctcta tctgcgcgcc
     4321 aaaaagcagc ccattctccg gattgccgac accatcatcc ccctcttctt tctcggtgag
     4381 gccatcacgc gcatcggctg ctacctgaac ggctgctgtt acggcatccc ctgtgacggc
     4441 cccctgtgcg tggtctttcc tcccgacagt ccggccgggg cgatgtacca gggcatccac
     4501 atccatcccg cccagctcta ctcctcggcc tatgccctgc tcgtcttcat catcatgctg
     4561 gtcatcgacc gcaagcccaa atacgacggc tttctgctct atctcttttt catcttttac
     4621 ggagtcggcc gctttatcat cgacctcttc cgctactatg agagttcgat gatcctgctt
     4681 cccctcgggg ccaaggggct gagcatgaac caggggatca gcctggcctt tatcgtcatc
     4741 ggcatctttt tgctggtccg gcaggagcgc cgggagcggg tgggggcggc gccaggtaaa
     4801 taataaaata tttcttgact ttaaagggcg acaatgatat tttaagttag gtaaaaatag
     4861 tacttttttg cccttttgag gccgtatgga tccggccctg catctttgca tctggcacac
     4921 gggtatcaac tgccactcaa gatctgtagg agagatgtat gaaaagagag cctattgtca
     4981 ggattctcgg cgggatggcg ttagcggcga tggttgtggt gtggagcggc tgcagcagct
     5041 cccggcaggt ctcgttggct acagaacagg ctgccctctc cggtgctgaa gagaaggcca
     5101 ccacagacac ccagacggcg aacaagagca gcgacgagga cgaggtgatg cgactgctcg
     5161 gcctcaacaa gagtgagagc gccgtcgagg cggcccctca gcccactgca gcgggtctcg
     5221 aagaggatcg caagcggctc gaggaggccc tcaaggagag ggacatcgag atcgccaatc
     5281 tcaaggcgga gctggccgag cgcgaccgcc agctcaacgc ggcggagagc gccgaaccgc
     5341 cgatggagat caagccggtc cggacccccg tctccggcag cggcaccttt caggagcgct
     5401 atgaccgggc gcgcgccctc tatgaatccc gccgctacaa ggaggccatt caggaattcg
     5461 ccgccctgct ctcgaccgat tcgagccaca agctcgccga caactgccag tactggatcg
     5521 gggagtgcta ctacggcatg cgcgcctaca accaggcgat cgttgaattc gaaaagatct
     5581 tcaccttcaa cgattccgac aagaatgacg acgcccagct caagcttggg ctctgctaca
     5641 cccgcatcgg caatgtcgaa aaggccaaag ccgagtttca gaagctggtc gatacctatc
     5701 ccgagagtga gttcatctcc cgggcgcagt actatctgca gaccctgtga ggcgggggga
     5761 ccggcaggct cgtcccgtcc gggggcagga gttttccatc agggtggcgc tggcgccacc
     5821 ctttttttta ttgcatttgt catgaatttt taataattta ttaagaccag aactttcttg
     5881 accttacatc tcgtcaattc cgtcacctcg tgcaaaaaat cggactgaac aaggatgaaa
     5941 aaccggctga gggttctcta tctatcgact gaagtagcac catttgccaa gaccgggggg
     6001 ctggccgatg tcgccagcgc tcttcccaag gctctctttg agcaggggca tgatgtccgg
     6061 gtgatgatgc ccaaatatgg caacatcagc gaaaggcgct ataccctgcg tgaggtcatc
     6121 cgtctcaagg acatccccat cggcgtcggc ggtcaggaac atgtcgtcag cgccaaatcg
     6181 gcttttcttc ccgagaccaa ggtccaggtc tatttcctcg attacaagcc cttcttcgaa
     6241 cgcagcgatt actacgtcga ctccgccacc ggcaaggact ttcccgacaa cgcccagcgc
     6301 tttttcctct tttgcaaggc ggttctggaa acgatcaagc tgctgcactg ggaaccccac
     6361 atcatccact gcaatgactg gcagaccgcc ctcatcccct ggctgttgaa gaaccactac
     6421 caggatgatc cctttttcgc caaggcgatc acggtgctct ccatccacaa tctcgccttt
     6481 caggggattt ttccgcagag cgtgcttccc gagctggggc tggcggcgga gctcggccag
     6541 gcgggcaccg atctcgagtt ttatggcaag gtcagctttc tcaaggccgg gatcaggagc
     6601 gccgatctga tcaccacggt cagcccgacc tactccgcgg agattcagag cgatcccgaa
     6661 ctgggctgcg gcctgcaggg tgtgctgctg gagcgcaagg aggacctgtg cggcatcctt
     6721 aatggagtcg attacggcat ctggaatccc gagaaggatg ctcttattcc ggtcaaatac
     6781 agtgctgcga ccctcaagga caaggagaag aacaaaaagg ccctcgccaa ggaggccggc
     6841 tttcccttcg atccggcggt gccggtcgtc ggcatgatct ccaggctgac cagccagaag
     6901 gggttcgacc tcgtcgccgc ggcgatcgac gacctgctca agctcaagat ccagctggtc
     6961 atcctcggca ccggcgatcc cgtctatcac aagcagctcg aaaaactggc caaggcccat
     7021 cccaagcagg tggccctctt tctcaaattt gatgataccc tcgcccacct gattgaggcg
     7081 ggcagcgata tgttcctgat gccctcgctc tacgagccct gcggcctcaa tcagatgtac
     7141 agcctcaaat acggcaccat cccgatcgtg cgcaagaccg gaggcctggc cgacaccatc
     7201 atcgacgccg atgcggatcc cactaacggc aatggctttg ttttcgctga atataaagcc
     7261 aaggcgatgg tcgatgcagt gcgccgcgcg gtcgcagcct tccgcgacga gaagggctgg
     7321 cagaagctga tcaaacgctg catgaagcag gatttctcat ggcagctcgc cgccgacaaa
     7381 tatatcaggc tctatctcaa actggaaacg gccaagaaga aaaaataggc cggataaaat
     7441 tcccggcccg cctcctgcga ctcgctatcc tcccctcaga catccggcat gcccgggcaa
     7501 atacccctgg atcccggggg tttttgtttt gcccccggct tgagattgtg tttcaatctg
     7561 atacaaatat gtcttgcaga acgacaaaaa agggcgacgg cggggcaaaa ccggcggcag
     7621 cgaacgcggg ggcttgcggc ggcaccatct tttaatataa aaagttacaa gtagatagac
     7681 cttcagcatc ctgcggcgcg aaccgcggga acgatggcct ggccgcagat ttgctggcat
     7741 taactttgca taaagagggc taacggccgc gccgggttac ggggagagga atgcccggct
     7801 gcgcgtccgg tcatggcgtc ttcaagcaaa caggcattgg atggatggta tagaaataac
     7861 agtagaccgt gtcggcgccc tgcgcgatat cggcttgttc cgggtccggg gctatgtgga
     7921 tacgacgacc tctcctgaat tgcagcgcat gctgaccgag atcattcacg acggggtcac
     7981 ccagatcatc atcgatctgg gggcggttaa ctatgtcagc agcgcggggt ggggggtatt
     8041 tgttggggag attcgcggcc tgcgcgagca gggcggtgat ctcaaaatta ttcagatgat
     8101 cccggaagtg aatgaagtgt tcgaaatgct cgagttcaat cggatcctga cctgctacga
     8161 ctcgctcgaa gaggccattg acgattacga cttttgtcag ggcgtggatt tgcgggcctc
     8221 cctggcagaa ggggccgccg ccggcaaggc ggtgccggcc tgggagacgg atcgtgttgt
     8281 cgaaaatccc gctgattaca tccccccggc gaacaaggaa agccgcacca accgcaatat
     8341 caccatcgcc cgaggccgag gcatcgacga tgtcgatctg cctctcaacg aaaaaatcaa
     8401 aaagctggtg accgagaacc cgctcatgac gccctggggc atcaagcggc agctcaactc
     8461 gcccaggttc ggctatacac gaatcgggtt tttcaagctg cgcggcatcc tgaagcggct
     8521 cggactggag accagggcga agcgctaccg cttttaccgc tcccgctgat ccggctcctc
     8581 cggccggaag gccttggatc ccttgttccc gttcctccac tcgccgggtt tcctggcggc
     8641 atacagaaga ggagattgaa atgaaacgcc cgttgttctt tgtttacggg tatatggcgg
     8701 ccctgctgct cggcgccgtt cagggaaatg cccaaaccgc atcgcccgag ttctctcccg
     8761 aggcgctgcg ggtgaccccg ggcgatgcga ttcatctcta catctacgag aatctcttcc
     8821 cctcggagaa gggtaagttc gtcagcaatt atcacgacaa ggagttcgtc gtcgacggcc
     8881 tgggccagat caccctgggc cccctcggcc gcgtgcagat cgcggggctc aaggcagagg
     8941 agatcgccca ggtcctgcag gaaaagttca agcccttcgc caaggacccc ttcatcatcg
     9001 tcgtccccct catccgcctc gaactcaagg ggggctttag ccagcccgga ctctaccgct
     9061 tcaatcccaa catctccttc tgggaggtga tcaaggaggt gggggggctg cactcgctct
     9121 cttcctatga ggatatgttc attctgcgca acggccaggc gctctatcgc ggattcgggg
     9181 atgcctttta tcgcggccag tcgctttacg aactcgggct gcagtcgggc gatgaaatcg
     9241 tcgccccgcg ggtgaaccgg ctttcgttcg ataccatcat ccgctacatc cagttcgcca
     9301 tgtccctgtt caccttttat tttgtcctga tcaattataa caagaatgac aattcaaatt
     9361 ccctctaacg gttttttgct gacatggatg atctcgatct tttaatgccc gccggcgagc
     9421 agcagtccgc tcccatggat ttcggcaagt ttttcaacgg gttctggaag cgcaagtgga
     9481 tcatcttgct cctgaccctc atcggcgcgg cctggttttt ctgggactcc aagaacgaaa
     9541 agccggtcta cagcaccttt gccaccatca aaacccgcag tttcgactcc gatgccgaga
     9601 gcattctcag ccgtgatcgt caggccgagc tgcgcagccg cagctttgcc gagcgcgtcg
     9661 ctgcgcgtat gggcctggcc ctgcagatgg ggaccaaggc cggccccctc ggcgatgttt
     9721 tcagcgagtt tcataccacc accagtccga ccccgggcaa gtacaagatc caggtcgatg
     9781 acaacggtac cttctccatt ttttcggtcg actatcccga ggagaccctg ctggacagca
     9841 tgagcgtctg ggatgtcatc tcccgcgagt acacctacga aggtttcact ttccgtctca
     9901 atcccaagtg ggttgagcac tcccagttcg tcaccttcaa gatccgtccc ttcgagcggg
     9961 cggtcgccga actgaacacc atggtcaccg ccgaggtgag caagaacgga aacctgatga
    10021 agctgaccat caagggggag gaccgcgatc atctcgccga ccagctcaac cagatcgccg
    10081 acatctacgt ccaggagacc ctgatgctca gggaccggga tgccaactct tatcgcaagc
    10141 ggctcgaggg cagcctcgag gtcgccgccg agaaagtgcg tgcggccgat gccgatctgc
    10201 gcaatttcta ttccaagtat cccctctccc tcgatgccgc caagaagacc atcctcgacg
    10261 agatgcagcg caacagctat accctgcgcg agatccccca gcagcgccag cagctgaccc
    10321 agctcctcga gaagctgggc gagccggtgg tcggttcgga cccggagcag taccggcggc
    10381 tgatcgtccg ccagctcgcc aatttttccg ccatgcgcga tgagcccgcc ctggccctgc
    10441 tgcgcgatca gctcgacagc caggagaagc agctccagga tctgatgcgc gactattcga
    10501 acgattatgc ccccctggtc agcctgcgca cgagcatccg cgagacccag gacaacatca
    10561 tcgccttcgc ctccaattac cgcaacaccc tggcgcaaaa ggagagcgag acccggatca
    10621 agatggcgga tatccagtcc cagcagcgca tgctgcccga ggacgagtat cgtctgatgg
    10681 agctggaacg caacaagaag gtcagcgagg accattacac cgccctttac accaagctgc
    10741 aaaagctgga gatcgccgat ggtggcgagg gcgaggacat tgcggttctg gaccgggcga
    10801 tcaagccgac cgtgccgatc aacccgagcc agtcgaccaa gatctttatc ggggccgcgc
    10861 tcggcctggt ggcggggctg gcgctgtcgc tcgggatcga tcttgccgac cgccacatcc
    10921 gcacccccgg ggatgtggag cgccacctca atctcaaggt gcttggggcg atccccaatg
    10981 tggatttcaa ggacatcccc gagtatcacg atcatgaaaa ggccaagcag atcgaccggc
    11041 agctggtgac ccatgattat tcgcccaccc cgatcggtga ggcctaccgc tccctgcgca
    11101 cccagatcat tttttcgcgg acccacgagc ggccccactc catcgccatc accagcgtcg
    11161 ccccggagga gggcaagtcc ttcaccgctt cgaatctggc gatcatcttc gcccagcagc
    11221 gcaccaacac cctgctggtc gacgccgatc tccgccgcgg ggtgctgcac aacacctttc
    11281 agatgcaccg cgagccgggg ctggccaact atctcagttc cagcgtcacc ctctcctcga
    11341 tcgtacagca gacccacgtg cccaatctct ccttcatcag ctgcggggtg ctgctgccca
    11401 acccctctga gctgctgggt tcgcccctga tgcgccgctt ccttgacgag gcgagccgca
    11461 agttcgatat cgtcctcttc gatacgcccc ctctggatgc ggcgaccgat gctgtcgtgc
    11521 tgggcaccca ggtcaacgcc gtcgtgctgg ttgtgcgcgc gggcaagacc aaaatcggca
    11581 aggccaagga gaagatggaa atctttcata ccgtccctgt caaactggtc ggtgcggtcc
    11641 tcaacagctc cgacacccag ctggtgcaga actacagcta ttaccattat
//
LOCUS       DAOPWU010000081        11659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5633_length_11659_cov_5.569545, whole genome shotgun sequence.
ACCESSION   DAOPWU010000081 DAOPWU010000000
VERSION     DAOPWU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5633_length_11659_cov_5.569545"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKM23_05990"
     CDS             complement(<1..634)
                     /locus_tag="PKM23_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNY91061.1"
                     /translation="MRILPLLLLMWTFAAAAAEPWTDLFNGRNLKNWRVLNGDAKFIV
                     RDGAIVGVSKLNTPNSFLATRASYDDFILEFECRIDDGLNSGVQIRSLSLPEYQNGRV
                     HGVQIELDTSPRAWSGGIYDEARRGWLYNLECNPAGKKAFKNGEWNRFRVEAIGTSIR
                     VWVNDIPTADIVDDLAPSGFIALQVHAIAGDASKAGKEIAWRNLRIMTSEI"
     gene            complement(665..1822)
                     /locus_tag="PKM23_05995"
     CDS             complement(665..1822)
                     /locus_tag="PKM23_05995"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HNY91062.1"
                     /translation="MKWIAWLLAGGAMMIPVLGSGCAGGKLPLLYVGKYNDRGGAGFQ
                     VVELDPGAGLFRLAAEVEAGANPSYFCIDGEQGLIHAINEVDELRGQKTGGITTLALD
                     AASGALRRTGEMAVPNGGPCYISRMPAGDYLLVANYGGGSVAVVRLDARGMPAAVTDT
                     LVFQGVSGRTPRAHMIAPGPDGNRIYLTDLGLDRIMIYELDRTAGKLQSVNNGEVALA
                     KGAGPRHFTFSKDGSTLYVINERNSTVTVFAVDAEGLLEEIQSLSTLPEGFSGTSYCA
                     DLHLGKSGRFLYGTNRGHNSIVTFRVVGNGRLELAGHTPCGGDWPRNFVIDPSGRYLV
                     VANQRSHNLALFAIDSKSGLPLLLTAAFTTTSPACLKFALRPGESAGGVQY"
     gene            2409..4127
                     /gene="ilvB"
                     /locus_tag="PKM23_06000"
     CDS             2409..4127
                     /gene="ilvB"
                     /locus_tag="PKM23_06000"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNY91063.1"
                     /translation="MTGAEMIIHALKREGVRALFGYPGGMVIDIFDALYSAGVPFYLT
                     RHEQGAAHAADGFARASGEVGVCLATSGPGATNLVTGLATAYMDSIPVVAITGQVPTS
                     LIGNDAFQEADMLGITRSITKHNFLVKEVDELPRLLKSAFYIARSGRPGPVLIDIPKD
                     IQQATTAMPYPDEVNIRSYKPTVAGHLPQIQKAAARISRAERPVIFAGGGVISSGASA
                     ELALLARRMQAPVTTSLMGLGAFPETDPLALKMVGMHGTAYANYAIQEADLLIAIGVR
                     FDDRVTGKVSKFAPKAEIIHIDIDPATIRKNVKVSIPLVGDIRCVLTELNQLVENRNH
                     DPWLQTVAGWKRENPLQYTQNGGISPQAVIRTIAEMTGHQAIIATEVGQNQMWSAQFY
                     DFISPRTWISSGGLGTMGFGFPAAIGAQVAHPGRLVIDIAGDGSIQMNIQELATAALY
                     NLPVKIVILNNRSLGMVRQWQSIFYQRRFSGVCLNRDARCPGECDGRSDSCPLYLPDF
                     VKLAAAYNIPGFRSERPEEVAPILEKAFAHDGPAIVEFRISQEENVFPMVPAGAGLNE
                     MLRGMA"
     gene            4127..4621
                     /gene="ilvN"
                     /locus_tag="PKM23_06005"
     CDS             4127..4621
                     /gene="ilvN"
                     /locus_tag="PKM23_06005"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNY91064.1"
                     /translation="MRHIINLEVENSAGVMARIVGLFSARGYNIESIVAGKTVEECTA
                     RITLVVIGDDKIIEQVNKQLNKLIDVIRVTDVTREDFVNRELLLVKIHCPPARRTEAS
                     LVGNIFGAKVVDISAKSLTFELTGSESKITAFLQTLRPFGIREVARTGPIAMVRDFNP
                     RSSE"
     gene            4748..5737
                     /gene="ilvC"
                     /locus_tag="PKM23_06010"
                     /pseudo
     CDS             4748..5737
                     /gene="ilvC"
                     /locus_tag="PKM23_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009546873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
     gene            5751..7304
                     /locus_tag="PKM23_06015"
     CDS             5751..7304
                     /locus_tag="PKM23_06015"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNY91065.1"
                     /translation="MPQDRILIFDTTLRDGEQSPGASLTVSEKLTIAEQLARLKVDAI
                     EAGFPVSSQAQFDGVRLIAGQVRGPAIVALARCIDGDIDAAWAALKGAERPRIHVFVA
                     TSRIHMEKKFRKSEEEILAMAVAGVRRARSLCQEVEFSPEDSSRTGVDFLLRIVEAAI
                     DAGATSINIPDTVGFAMPEQFGALIRTVREKVPNMGRAVLSVHCHNDLGMAVANTLAA
                     IRNGAGQVEVTVNGVGERAGNASLEEVVMALKTRADFYGKSSGVETREIMRTSRLVSS
                     LMGIPVQPNKAIVGANAFAHESGIHQDAVIKDAATYEIMTPQEVGLSSNAIVLGRHSG
                     RHGLKNRLAELGYALQPAELDVIYQRFLEIADRKKEVFDEDLLALMSAETGETAEIWK
                     LDYFHILSGNKTVPTATVVLRRGEESLQEAAVGDGPVDAAYNALNRIVDLAVTLQDYN
                     LRGVTGGKEAVGEVVVRVRHQGTTWLGRGASTDVIEASIRAYLNALNRILAAGSSPAR
                     PGDDQGDSL"
     gene            7301..8365
                     /gene="leuB"
                     /locus_tag="PKM23_06020"
     CDS             7301..8365
                     /gene="leuB"
                     /locus_tag="PKM23_06020"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNY91066.1"
                     /translation="MKKQITILPGDGIGPEVVHEAVKVLEAIGGKSKLELTLSEALVG
                     GAALEKAGEPLPAATLDLCRRSDAVLLGAIGHPRWDSMPPERRPERGLLGLRGELGLY
                     ANLRPARVYPSLAEASSLKAELVRDIDILIVRELTGGLYFGQPRGIEADRGYNTMIYH
                     RHEVERIARTAFEAARRRRGKVTSVDKANVLEVSQFWRQVVVEVAREYPDVTLDHIYV
                     DNCAMQLVRNPGQFDVLLTENMFGDILSDEAAMLTGSLGMLPSASLGSGTALYEPVHG
                     SAPDIAGQGAANPIATISSVGMMLRYSLNMAAEADKLDQAINRVLESGARTRDIAPPG
                     SAWITTAAMGAKIRTAYLEM"
     gene            8380..9957
                     /gene="cimA"
                     /locus_tag="PKM23_06025"
     CDS             8380..9957
                     /gene="cimA"
                     /locus_tag="PKM23_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNY91067.1"
                     /translation="MSDRLIVYDTTLRDGGQSEGISFSADDKLKIAQRLDAFGVDYIE
                     GGWPGSNPKDLEFFRRAAGLAWKNARIAAFGSTRKVNVPAGEDPNLRALLEAGTPVTT
                     IFGKSWDLHVIEGLRTTLEENLHLIRDSVAFLHDHGKEVIYDAEHFFDGYLADPEYAL
                     RTLAAAREAGAACIVLCDTNGGTLTLRLAGIIRAVRERFPEMPLGLHAHNDGDVAVAN
                     SLAALGLGVRHIQGTINGYGERCGNANLCSIIAGALLKLEIPLATAVALHELTGLSRY
                     VSEIANLPHREESPYVGNSAFAHKAGIHVSAIQRNRRTYEHVDPERVGNHRRVLVSDQ
                     SGQSNILYKAGEMGIDITSDRAEVRRLVEQLKEMEHLGYQYEGADGSLEVLIKKSAGL
                     YTPYFSLESARVIVDKSENGFLRAEAILKISVKGQIEHTAAEGHGPVDSLDRALRKAL
                     HPFYPGLERVKLADYKVRVLNSDLATAATVRVYIESTDGKSSWGTVGVSENIIEASWQ
                     ALSDSIDYYLMRTINGL"
     gene            10079..11209
                     /locus_tag="PKM23_06030"
     CDS             10079..11209
                     /locus_tag="PKM23_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNY91068.1"
                     /translation="MKKIMVLGAGLVGRAMAIDLCRDHEVTSVDIHADALARLAAEHP
                     IRTVTADLGRKEAITSLAAGQDLVIGALPGFMGYRALEAVISAGVNCIDISFFPEDPF
                     GLDGMAKAGGVTVVTDCGVAPGMSNLIIGHHQRSMRIDSFECLVGGLPKKRTWPFEYK
                     APFSPLDVIEEYLRPARLMENGRIVTKPALSEPELVELEPVGTLEAFNTDGLRTLLTT
                     ANIPDMREKTLRYPGHCQLMRILREAGFFSDEPIPVGGAMVSPREVTSRLLLPQWQLG
                     EEEIEFTIMRITLEGEEGGRRLRHIYRLYDTYDPATRTSSMARTTGYTATAAARLVLE
                     GRFVRKGISPPEYLGAEGECFERIMADLGRHSVRYTHEETVL"
     gene            11410..>11659
                     /locus_tag="PKM23_06035"
     CDS             11410..>11659
                     /locus_tag="PKM23_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91069.1"
                     /translation="MHNPFIKRLFLNHPTIISVIEEAETPQKARNYLKAYLEEYSRFL
                     SKGSHRDSSLAWSQQITCLRVFQEMISIRSERISHFSII"
BASE COUNT         2247 a         3927 c         3479 g         2006 t
ORIGIN      
        1 cgatttcgct ggtcataatg cgcaggttgc gccaggcgat ctccttgccc gccttgctcg
       61 catcgccagc gatggcgtgc acctggaggg cgatgaagcc ggagggggcc aggtcatcga
      121 cgatgtcggc ggtgggaata tcgttgaccc agacacggat cgaggtgccg atcgcctcga
      181 cgcggaaccg gttccactcc ccgtttttaa aggccttctt gccggccgga ttgcattcga
      241 ggttatagag ccaaccccgg cgggcctcat cgtaaatgcc gccgctccag gcgcgcggcg
      301 aggtatccag ttcgatttgc acaccgtgca cgcggccgtt ctgatactcc ggcaggctga
      361 ggctgcgaat ctgcacgccg gaattgaggc cgtcatcaat ccggcattca aattcgagga
      421 tgaaatcgtc gtagctcgcc cgtgtcgcca gaaagctgtt cggggtgttg agcttgctca
      481 cgccgacgat tgcgccgtcc cgaacgatga atttggcatc cccgttgagc acccgccaat
      541 tcttcagatt cctgccattg aaaaggtcgg tccagggctc agcggccgcc gcagcgaagg
      601 tccacatcag cagcaacagg ggaagaattc tcatcaacgg ctcctttccg gggaagatca
      661 tactctaata ctgcacacca ccggcggact cgccgggccg aagggcgaat ttcaggcagg
      721 ccggcgaggt ggtcgtaaag gccgccgtca gaagaagggg aagcccgctt ttgctgtcga
      781 tggcgaagag ggcgagatta tgcgagcgct ggttggccac caccagatag cggcccgatg
      841 gatcgatgac gaaattgcgc ggccaatccc ccccgcaggg ggtatgcccg gcgagttcca
      901 gccggccgtt cccgacgacg cggaaggtga cgatggagtt atggccgcgg ttggtgccat
      961 agagaaaccg gccgctcttg ccgagatgga ggtcggcgca gtagctggtt ccggaaaaac
     1021 cctcgggcag ggtgctgagg ctctggatct cctcgagcag tccctccgca tcgacggcga
     1081 aaaccgttac ggtcgagttg cgttcgttga tcacatagag ggttgaacca tctttgctga
     1141 aagtgaaatg gcgcggaccg gcccccttgg ccagggccac ctcgccgttg ttgaccgact
     1201 gcagcttgcc ggcagtgcgg tcgagttcgt aaatcatgat gcggtcgagg ccgaggtcgg
     1261 tgagatagat cctgtttccg tccggcccgg gcgcgatcat gtgggcgcgc ggcgtcctcc
     1321 ccgacacacc ctgaaaaacg agggtgtcag tcactgcggc gggcataccc cgggcatcga
     1381 ggcggacgac tgcgaccgag ccgccgccgt agttggcgac caggagatag tccccggccg
     1441 gcatccgcga gatatagcaa gggccgccgt tgggcacggc catctctccc gtccggcgca
     1501 gtgcgcccga ggcggcgtcg agggccaggg tggtgatccc gcctgtcttt tgcccccgga
     1561 gttcatcgac ctcgttgatg gcatggatca ggccctgttc gccatcgatg caaaagtagg
     1621 aggggttggc cccggcctca acctcggcag cgagcctgaa gagccccgcg ccgggatcga
     1681 gctcaacaac ctgaaatccc gccccgcccc tgtcgttgta cttgccgacg taaaggaggg
     1741 gcagcttccc cccggcgcag ccgctcccca gaaccgggat catcatcgca cctcccgcca
     1801 gcagccatgc tatccatttc ataacattct ccgtgcctag tcgatttttt gccgctgatg
     1861 gcctctatcc gggaaaatag cacttcacgt gcttaaaaac aagcactatc cacaaccggg
     1921 cggagtgcaa accggaatcg ggcgcggatc cagcagttgc gaccggtcag ccgagcacaa
     1981 tccgagcaag cgatcagggt aaaataattt ctccgccgca gaaatttagt ttgacattgg
     2041 ggtgattttc ggttatattc aaagaacaga ccctatcaac ccggcaggaa aaacaaaatg
     2101 cattatgcgc atcacatcgc tgttgtccgc tttcatgact ccgctcatga cggccagggt
     2161 gtgctgcgag cccacctcgc tctatccaca gactccctgc gggcgatcgc tattctgcat
     2221 ctcattctta ttaacctctt gcttctgatt atctactaaa tcaaggctgc aagaggaaaa
     2281 ccgcacaccg cttcatcacc cgtcaacact ccacaccatc cgcgcatacc ctctctgcag
     2341 ccacaggacc ataacggctg tccgtccttt atagcccggc ggcggccccc agcagaagga
     2401 acaccagcat gaccggcgca gagatgatca ttcacgcctt gaagcgcgag ggggtccgcg
     2461 ccctcttcgg atatccgggg ggcatggtga tcgatatctt cgatgccctc tattcggcgg
     2521 gggttccctt ttacctcacc cgtcatgagc agggggccgc gcatgccgcc gacggcttcg
     2581 cccgcgcctc cggcgaggtc ggggtctgtc tggccacctc cggccccggc gccaccaatc
     2641 tggtcaccgg gctggccacc gcctacatgg actccatccc tgtggtggcc atcaccgggc
     2701 aggtcccgac cagcctgatc ggcaacgacg cctttcagga ggcggacatg ctcggcatca
     2761 cccgctcgat caccaaacac aatttcctcg tcaaggaggt cgacgagctc ccccgcctgc
     2821 tcaaatcggc cttttatatc gcccgcagcg gccgcccggg cccggtcctc atcgacatcc
     2881 ccaaggacat ccagcaggcc acgaccgcca tgccctatcc cgacgaggtc aacatccgca
     2941 gctacaaacc caccgtcgcc ggccacttgc cccagatcca gaaggcggcc gcccgcatca
     3001 gtcgcgccga acggccggtc atcttcgccg gcggcggggt catctccagc ggcgcctccg
     3061 ccgagctggc cctgctcgcc cgccgcatgc aggcgccggt gaccacctcg ctgatggggt
     3121 tgggggcctt tcccgagacc gaccccctcg ccctcaagat ggtcggcatg cacggcaccg
     3181 cttacgccaa ttatgccatc caggaggccg acctcctcat cgccatcgga gtgcgctttg
     3241 atgaccgcgt caccggcaag gtcagcaaat tcgcccccaa agcggagatc atccacatcg
     3301 atatcgatcc ggccaccatc cgcaaaaacg tcaaggtctc catccctctg gtcggggata
     3361 tccgctgcgt gctcaccgaa ctcaaccagc tggtcgagaa ccggaaccac gatccctggc
     3421 tgcagacagt cgcgggctgg aaaagggaaa atcccctgca gtacacccag aacggcggca
     3481 tctcgccgca ggcggtgatc cgcaccatcg ccgagatgac cgggcaccag gcgatcatcg
     3541 ccacggaagt ggggcagaac cagatgtgga gcgcccagtt ctacgatttc atctctccgc
     3601 gcacctggat cagctcgggc ggcctcggca ccatgggatt cggcttcccg gcggctatcg
     3661 gggcgcaggt ggcccatccc ggccggctgg tcatcgacat cgccggcgac ggcagcatcc
     3721 agatgaacat ccaggaactg gccaccgccg ctctctacaa cctgccggtc aagatcgtca
     3781 tcctcaataa ccgctcgctc ggcatggtgc gccagtggca gtccatattc taccagcgcc
     3841 gtttctcggg ggtctgcctc aaccgcgacg cccgctgccc cggggagtgc gatggccgca
     3901 gcgactcctg tcccctctat ctgcccgatt tcgtcaagct ggccgcggct tacaacatcc
     3961 cgggcttccg ctcggagagg cccgaggagg tggcgccgat cctggaaaaa gccttcgccc
     4021 atgatggccc cgccattgtc gaattccgga tcagccaaga ggagaatgtc ttcccgatgg
     4081 tgccggcggg agcgggactg aacgaaatgc tgagagggat ggcctgatga gacatatcat
     4141 caatctggaa gtggagaaca gcgccggggt gatggcgcgc atcgtcgggc tcttcagcgc
     4201 ccgcggttac aacatcgaga gcatcgtcgc cggcaaaacg gtggaagagt gcaccgcccg
     4261 catcaccctg gtggtgatcg gcgacgacaa gatcatcgag caggtcaaca agcagctcaa
     4321 caagctgatc gacgtcatcc gcgtcaccga tgtgaccagg gaggatttcg tcaaccgcga
     4381 gctgctgctg gtcaagatcc actgcccgcc ggccaggcgg accgaggcct ccctggtcgg
     4441 caatatattc ggggccaagg tggtcgacat atcagccaaa tccctcacct tcgaattgac
     4501 cggcagcgag tccaagatca ctgcttttct gcagaccctg cggcccttcg gcatcaggga
     4561 ggtggcccgc accggtccga tcgccatggt gcgcgatttc aatcccagga gcagtgagtg
     4621 aaagcgttcc acacggagtc acagagacac cgtggaggca aaacagctct attctatccc
     4681 ggtgacctcc gcgccaccat gagaaattta ttgtcgccct gtaaatcatt ttagcaaagg
     4741 agatctcatg aaactttatt atgaacagga tgccgatccc ggcttgctgc agagcaagac
     4801 cgtggctgtg ctcggttacg gcagccaggg ccatgcccac gccctcaacc tgcgcgacag
     4861 cggcgtcagg gttgtcgtcg gcctgtacgc cggcagccgc tcgcaggccg cggccgaaaa
     4921 ggaggggctg caggtgctgc ccgtgcgcga agcctgcgcc gccgccgatc tgatcatggt
     4981 gctgctgccc gaccagaccc aacctgtggt ctatgccgaa tcgatcgcgc ccgccctcgc
     5041 tcccggcaag gtgctggcct tcgcccacgg cttcaacatc cattacagcc agatcgtccc
     5101 tcccgccggg gtcgatgtca tcatgatcgc cccaaaatcc ccgggccatc tcgtgcgccg
     5161 ggtctaccag gagggcaacg gcgtcccctg cctgatcgcc gtgcaccaga atccctccgg
     5221 ccgggcgcgc gacatcgccc tggcctatgc ccgggggatc ggcggcaccc gcgccggggt
     5281 actcgaaacc tccttccggg aggagactga aaccgatctt ttcggcgagc aggcggtgct
     5341 ctgcggcggg gtcaccgagc tgatccgcgc cggctatcag accctggtcg aggccggcta
     5401 cgagccggag atcgcctatt tcgagtgtct tcacgaactc aagctcatcg tcgacctcat
     5461 caacgagggg gggctcaccc gcatgtacta ctcggtcagc gacaccgccg agttcggcgg
     5521 gctgacgcgc gggccgcggg tgatcggtgc ggagagccgc cgggccatgg ctgacatcct
     5581 cgccgagatc cgcagcggcc agttcgccac cgagtgggtg ctggaaaacc aggcccatgc
     5641 accgcgcttc aaggccctgc gccggatgat gcaggagtgg ccgattgaaa aggtgggggc
     5701 gcagctgcgc agcatgatga gctggctgaa aaatagaaag gaggcacatc gtgccgcaag
     5761 atcgaattct gattttcgac accaccctgc gcgacggcga gcaatccccc ggcgccagcc
     5821 tgaccgtaag cgaaaagctg accatcgccg agcagctcgc ccgcctcaag gtcgatgcca
     5881 tcgaggccgg ctttccggtc tcctcacagg cccagttcga tggagtgcgg ctcatcgccg
     5941 ggcaggtccg ggggccggcc attgtcgccc tcgcccgctg catcgacggg gatatcgatg
     6001 cggcctgggc ggccctgaag ggggcagaac ggccgcggat tcacgtcttt gtggccacct
     6061 cccggatcca catggagaag aaattccgca agagcgagga ggagatcctc gccatggccg
     6121 tcgccggggt gcggagggcc aggtcactct gtcaggaggt cgagttttcg cccgaggact
     6181 cttcccggac cggggtcgat tttctcctcc gcatcgtcga ggcggccatc gatgccggtg
     6241 ccacctcgat caacatcccc gacaccgtag gtttcgccat gcccgagcag ttcggggccc
     6301 tgatccgcac cgtacgcgaa aaggtcccca acatggggcg ggcggtgctc agtgtccact
     6361 gccacaacga tctcggcatg gcggttgcca ataccctggc cgcgatccgg aacggggccg
     6421 gccaggtgga ggtgaccgtc aacggagtcg gtgagcgcgc cggcaatgcc tcgctcgagg
     6481 aggtggtcat ggccctcaag acgcgggctg atttctacgg caaaagcagc ggggtcgaga
     6541 cccgcgagat catgcgcacc agccgcctgg tctcctccct gatgggcatt ccggtgcagc
     6601 ccaacaaggc gatcgtcggc gccaacgcct tcgcccacga atcgggtatc catcaggatg
     6661 cagtgatcaa ggacgccgcc acctacgaga tcatgacccc ccaggaggtc ggcctctcct
     6721 ccaatgccat cgtcctcggc cgccactccg gccgccacgg ccttaaaaac cgtctggccg
     6781 agctgggcta cgccctccag cccgccgaac tcgatgttat ctatcagcgc tttctcgaga
     6841 tcgccgaccg caagaaggag gtctttgacg aggatctcct cgccctgatg agcgccgaga
     6901 ccggcgagac cgccgagatc tggaaactcg actactttca catcctgtcg ggcaacaaga
     6961 cagtgcccac cgcgaccgtg gtgctgcgcc gcggcgagga gagcctgcag gaggccgccg
     7021 tcggcgacgg cccggtcgac gccgcatata acgccctcaa tcgcattgtt gatctggcgg
     7081 tgacactgca ggactataat ctgcgcgggg tcaccggggg caaggaggcg gtcggcgagg
     7141 tggtcgtccg cgtccgccat caaggcacga cctggctcgg ccgcggcgcc tccaccgacg
     7201 tcatcgaggc ctcgatccgc gcctatctca acgccctcaa ccgcatcctg gccgcggggt
     7261 catcccccgc ccggccgggg gatgaccagg gagactcgct atgaaaaagc agatcaccat
     7321 ccttcccggt gacggcatcg gcccggaggt agtccatgaa gctgtgaagg tgctcgaggc
     7381 cattggaggc aaatccaaac tcgaactcac cctgagcgag gccctggtcg gcggggcggc
     7441 ccttgagaag gccggggagc ccctgccggc ggccaccctc gacctctgca ggcgcagcga
     7501 cgcggtgctg ctcggggcga tcggccatcc ccgctgggac agcatgcccc ctgagcggcg
     7561 cccggaacgc ggactgctcg gcctgcgcgg cgagctcggc ctctatgcca atctccgccc
     7621 ggcccgcgtc tatcccagcc tggcggaggc ctcgagcctc aaggctgaac tggtgcgcga
     7681 tatcgacatc ctcatcgtgc gcgagctcac cgggggcctc tacttcggcc agccgcgcgg
     7741 gatcgaggcc gaccgcggct acaataccat gatctatcac cgccacgagg tcgaacgcat
     7801 cgcccgcacc gcctttgagg ccgcccgccg gcgccggggc aaggtgacct cggttgacaa
     7861 ggccaatgtc ctcgaggtct cccagttctg gcgtcaggtc gtggtcgagg tcgcccgcga
     7921 gtatcccgat gtcaccctcg accacatcta cgtcgacaac tgcgccatgc agctggtgcg
     7981 caatccgggc cagttcgacg tcctcctcac cgagaacatg ttcggggata tcctcagcga
     8041 cgaggccgcc atgctcaccg gctcgctggg catgctcccc tcggccagcc tcggcagcgg
     8101 caccgccctc tatgagcccg tgcacggctc ggcgcccgac atcgccggcc agggtgcggc
     8161 caatccgatc gccaccatct cttcggtcgg gatgatgctg cgctacagcc tcaacatggc
     8221 cgcggaggcc gacaaactcg atcaggcgat caaccgggtc ctcgagagcg gagcacgcac
     8281 ccgcgacatc gcaccgcccg gctcggcctg gatcaccacc gctgcgatgg gggcgaagat
     8341 ccgcacggcc tatctggaaa tgtgagcaag ggagacccta tgagcgaccg gctgattgtt
     8401 tacgacacca ccctgcgcga cggcgggcag agcgaaggga tttccttctc ggccgacgac
     8461 aaactcaaga tcgcacagcg gctcgatgcc ttcggcgtgg attacatcga gggcggctgg
     8521 ccgggctcca atcccaagga tctcgagttt ttccgccgcg ccgccgggct ggcctggaaa
     8581 aacgcccgca tcgccgcctt cggcagcacc cgcaaggtca atgtcccggc cggggaggac
     8641 cccaacctcc gcgccttgct cgaggccggc accccagtca ccaccatctt cggcaaatca
     8701 tgggacctgc atgtcatcga ggggctgcgc accaccctgg aggagaacct ccatctcatc
     8761 cgcgattcgg tcgcctttct ccacgaccac ggcaaggagg tcatctatga cgccgaacac
     8821 ttttttgacg gttatctcgc cgatcccgag tacgccctcc gcaccctcgc cgccgcgcgg
     8881 gaggcgggtg ccgcctgcat cgtcctctgc gacaccaacg gcggcaccct caccctcagg
     8941 ctggccggga tcatccgggc ggtgcgggag cgcttcccgg agatgccgct tggcctgcat
     9001 gcccacaacg acggcgacgt cgctgtagcc aacagcctcg ccgctctcgg actcggggtg
     9061 cgccacatcc aggggacgat caacggctac ggagagcgct gcggcaacgc caatctctgt
     9121 tcgatcatcg ccggcgccct gctcaagctg gagatccccc tcgcgacggc ggtcgccctg
     9181 cacgaactga ccggcctctc ccgctacgtc agcgagatcg ccaatctccc ccaccgtgag
     9241 gagagtccct atgtcggcaa cagcgccttc gcccacaagg cggggatcca cgtcagcgcc
     9301 atccagcgca accgccgcac ctatgagcat gtcgatccgg agcgggtcgg caaccaccgc
     9361 cgcgtcctgg tctcggacca atccggccag agcaacatcc tctacaaggc cggcgaaatg
     9421 ggcatcgaca tcaccagcga ccgggctgag gtccggcggc tggtcgaaca gctcaaggag
     9481 atggagcacc tcggctacca gtacgaaggg gccgacggct ccctcgaggt gctgattaaa
     9541 aagagcgccg gcctgtacac cccctacttc tccctcgaaa gcgcccgggt gatcgtcgac
     9601 aagagcgaaa acggctttct ccgcgccgag gccatcctca agatcagcgt caagggtcag
     9661 attgaacata ccgccgccga ggggcacggg ccggtcgact ccctggaccg cgccctgcgc
     9721 aaggccctcc atccctttta ccctgggctg gaaagggtca agctggcgga ttataaggtg
     9781 cgcgtgctca acagcgacct cgccaccgca gccaccgtgc gcgtctatat cgaatcgacc
     9841 gacggcaaga gctcctgggg caccgtgggg gtttcggaga acatcatcga ggccagctgg
     9901 caggccctct ccgacagcat cgactattac ctgatgcgga ccatcaacgg gttgtgatac
     9961 ggaggggagc gccggcagca aagaccttgt aaatgttatt aaaatatttt accttggacc
    10021 agcatatcca gcccggcaga cccggggagc ggaactcagc caacagaaag aggttccaat
    10081 gaaaaagata atggtgctcg gcgccggact ggtcggcagg gccatggcca tcgacctctg
    10141 ccgcgaccat gaggtcacct cagtggacat ccacgccgac gcgctggcca ggctggccgc
    10201 cgaacacccg atccggaccg tgacggccga cctcggacgg aaggaggcga tcacctccct
    10261 ggccgccggc caggatctgg ttatcggcgc cctccccggc tttatgggct accgggccct
    10321 ggaagcggtg atctccgccg gagtcaactg catcgatatc tccttcttcc ccgaggaccc
    10381 cttcggcctc gatggcatgg cgaaggccgg gggtgtcacc gtcgtgaccg actgcggcgt
    10441 ggcccccggc atgagcaacc tcatcatcgg ccatcatcaa cgctccatgc gcatcgactc
    10501 cttcgagtgc ctggtgggag gcctgccgaa aaaacggacc tggccttttg agtacaaggc
    10561 ccccttctcc cctctcgatg tgatcgagga gtatctccgt ccggcccggc tgatggaaaa
    10621 cggacgcatt gtcaccaaac cggccctctc cgagccggag ctggtggaac tcgagccggt
    10681 cggcaccctc gaggccttca acaccgacgg gctgcgcacc ctgctcacca cggcgaacat
    10741 cccagacatg cgcgaaaaaa ccctgcgcta cccgggccac tgccagctca tgcgcatcct
    10801 gcgcgaagcc ggctttttct cggacgagcc gatccccgtc ggcggcgcca tggtctcgcc
    10861 gcgcgaggtg accagccggc tgcttttgcc ccaatggcag ctgggcgagg aggagatcga
    10921 gttcaccatc atgcgcatca ccctcgaagg cgaggagggc ggacggcggc tgaggcatat
    10981 ctatcgcctc tacgacacct acgatccggc cacccgcacc tcctcgatgg cgcgcacgac
    11041 cggctatacc gccacagccg cggcccggct ggttctggag ggacggttcg tccgcaaggg
    11101 catcagcccg cccgaatacc tcggcgcaga gggtgagtgc ttcgagcgca tcatggccga
    11161 ccttggcaga cacagcgtcc gctacacaca tgaggagacc gtgctttagg cggtcttcag
    11221 ccacgccctg cgggtgaaag cgcactgtgg gcatgctgcg cggcacggat tggcctgcct
    11281 cggaaaaagc tttcatcttt gcctgcaatg acctatattt aattattatc cacggctgac
    11341 aagtcacttt cttcattgag gagagcaaga acacatgaaa gaataatcca tccttttgga
    11401 ggtatcctca tgcataatcc attcatcaag cgccttttcc tgaaccatcc caccatcatc
    11461 agtgtgatcg aagaggcgga aaccccgcaa aaagcgcgca actatctcaa ggcctatctc
    11521 gaagagtact cccgctttct gtccaagggc agccatcgcg acagcagcct ggcctggtcg
    11581 cagcagatca cttgtctccg cgtctttcag gagatgatct cgattcgcag cgagcggatc
    11641 agccatttca gcatcatca
//
LOCUS       DAOPWU010000082        11534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5698_length_11534_cov_5.160293, whole genome shotgun sequence.
ACCESSION   DAOPWU010000082 DAOPWU010000000
VERSION     DAOPWU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5698_length_11534_cov_5.160293"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1681
                     /locus_tag="PKM23_06040"
     CDS             122..1681
                     /locus_tag="PKM23_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNY91070.1"
                     /translation="MRKKMTLLLLLGLLLPGRAQTLKEAFVDTLVERTLQTFNVPGIA
                     VAVVKDEEVVLAKGYGVRSILTRQPVDENTLFAIASNTKAFTAAALGILVDEEKLTWD
                     THVTDFIPEFRLHDPWVTAEFTIRDLLTHRSGLGLGAGDLMLFPDSARFTLPEIIHNL
                     RYLKPVSSFRTKYDYDNLLYLVAGEVVARASGMSWEEFVETRIMKPLGMTHSAASIDR
                     LPSAANIIDAHVMVDGRLQVVPLERGSVINAAGGIYSTVSDMAEWVQLQINKGAYDEA
                     PGKRIFSEQVHSEMWQPQTILSVRQPGSYNTHFAAYGLGWGVADMKGFLEASHTGGLM
                     GIVTKVTILPELKLGIIVFTNQQEGAAFAAITNTIKDFYLGMEFTDRVKELSERRQQQ
                     LGEASGIMAAVEKKIAEQRKKKGGVPDRVPYVGTYTDKWFGEVAITLEGERMRFRALR
                     SPKLRGEMFYYAGNTFIVRWDDRTLEADAFASFTLDEEGVPTGMTMKAVSPFTDFSFD
                     FQDLDFIRKTE"
     gene            1691..2317
                     /locus_tag="PKM23_06045"
     CDS             1691..2317
                     /locus_tag="PKM23_06045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY91071.1"
                     /translation="MACRNLIRRHFAEQLIRPHGLVGRWIIGPLWNRRNRVLNDKTLQ
                     ALRLREHEQVLEIGFGGGRLLAQMAKIVTHGHLSGLDASPIMVNRVRRKFQALVYKGI
                     MDIRLGSAEKLPFTDSAFDKVASVNAIFYWRDPARGIREACRVLRPAGLFVLTFTAKE
                     DLDRQEFGIPSFYEDEVISMLRKAGFYRIDVSRDRDAHRAFLILTAEK"
     gene            2331..2777
                     /locus_tag="PKM23_06050"
     CDS             2331..2777
                     /locus_tag="PKM23_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNY91072.1"
                     /translation="MQRVLIILGILTATLSGCTTKQPELGAEALRQQVIATEQAFAAT
                     MADRNLAGFISFLSEETVFFSGPKPLRGAKAVAEWWQKYYQGASAPFSWAPEQVEVLD
                     SGSLALSSGPVYDREGKLTGTFTSIWRLESPGKWRIIFDKGCPVCD"
     gene            complement(2874..3305)
                     /locus_tag="PKM23_06055"
     CDS             complement(2874..3305)
                     /locus_tag="PKM23_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNY91073.1"
                     /translation="MEQNMLQRLARSSSDKVIAGVCGGLGEHTGVPAWTWRLLFVFAF
                     ICFGTGALLYILMWIFMPPASASGPAQAPRNSWLSEFRRSEKDRRIAGICGGLGEHSD
                     VPSWTWRVIFVTLAFFYCFGVLAYILLWIFVPRSADLPQSV"
     gene            3585..3881
                     /locus_tag="PKM23_06060"
     CDS             3585..3881
                     /locus_tag="PKM23_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNY91074.1"
                     /translation="MTPKRKLKNPEDPQTIPGVGPNIAAHFGELGIHRVSQLRDADPE
                     GMYTRLCELHGGPIDRCLLYVFREAVYYASHDRHDPELLKWWNWKEGAAAYTKN"
     gene            complement(3935..5158)
                     /locus_tag="PKM23_06065"
     CDS             complement(3935..5158)
                     /locus_tag="PKM23_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNY91075.1"
                     /translation="MKRILFSSLLFLFILSPLLAAQTPAEIELVESIPVETCLDNPDI
                     RNTLEVWLELIEGAHSTIDLEQFYLSHQAGESLDQVITALQAAARRGVKVRILADARM
                     YKTYPVLIDSLGKWENITARLIDFGTLAGGIQHAKYMVIDGESLFLGSQNFDWRALTH
                     IHELGIRIRSTQVVSIYQELFELDWRLADGTPADTSRPVIQPRLYETPIRLLTSEYGP
                     AIIQPTFSPRALIPDTTLWCEEQIVRLLDGAQREIALQFLSYSPRSRNGGAEDPFDGA
                     LRRAAARNVRVRLLVADWQKGSRAEAALKELAQLPNIEVCFSVIPEWSGGYISYARVE
                     HCKYILVDGEAFWLGTSNAARSYFHTSRNAGVIVRSTPLAGRLLQIFNQSWESPYAER
                     IDPEASYAPRRHDGE"
     gene            5449..7257
                     /locus_tag="PKM23_06070"
     CDS             5449..7257
                     /locus_tag="PKM23_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNY91076.1"
                     /translation="MTFHKFFTWLILFSAAFLFSAKSHTEEQKWLTVYEKSGGLETPR
                     YAETMAYFERLDQASPFARLESFGKSPEGRSLPLFIISSDKAFTPEMARHTNKPVVLI
                     QGAIHSGESEGKDAVMLLARDLLIYKKHRELFENFIILIIPIFNVDGHERFSAYNRIN
                     QNGPQEMGWRVTSTRLNLNRDFMKADAPEMRQWLQMYHRWLPHLFYDCHTTDGLDFQY
                     LLTWNIDEHEQFGGAVSRWTRDTFLPGVGKACETQGLLIAPYADMLEDERPEKGFVIG
                     VWRPMLSTTYATVCNRAGFLIETHSLKPYGTRVQATLDFILIGLQEIVKNPHALLQAV
                     QEEDQRVSAWGRVPADTLKVPLQFRTRFDRGESALFYGYRVEIKPGAVSGRDYPVYTS
                     DKVEIPTVFYNEVEPTAEVAPPRGYLVPSGWQAIIDILRLHGISMYRLKEEYRGLFEG
                     YRFREVRFMPRSYEGRSIPSYRVEAVTEKRVFPEGSVYVPLGNPRAKLIMHLLEPEAP
                     DALVAWGLMNTIFEDREYFESYVMEPLAQKMMVDDPGLAALFTQRVHSDSAFAANPRA
                     RLGFFYQRSPWYDQDKDRYPVWRVTGDRPPALEELQ"
     gene            complement(7367..8539)
                     /locus_tag="PKM23_06075"
     CDS             complement(7367..8539)
                     /locus_tag="PKM23_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91077.1"
                     /translation="MYITREIEKTVVLLAAQYPIVTITGPRQSGKTTLVKKLFPEKPY
                     FSLENPDLRETVQSDPRAFLNRIQQGAILDEVQRVPEFFSYLQEVVDSRPLKGQYILT
                     GSNQFALLNSLTQSLAGRTVLLKLLPLTINELEKQTGGASTDDYLLNGFYPAIYGDRL
                     NPSITYRSYYETYIERDLRQLIHIKDLLQFQRFVRLCAGRVGQVFNASSLANEVGTSA
                     VTIKSWLSILQASYIVMLLPPYFENFGKRLIKAAKLYFYDVGLATYLLGIESTQQLAR
                     DPLRGALFENMVLMELIKTRTNRGLDPQFHFYRDNHQNEIDILFKRGSDLLPVEIKSA
                     QTFHADFLKGLRYLQKIMPERFKEGYLVYDGQIEQKIDTFDLVNFRRAAPVIFPPA"
     gene            8766..9389
                     /locus_tag="PKM23_06080"
     CDS             8766..9389
                     /locus_tag="PKM23_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY91078.1"
                     /translation="MNDFDARARTWDDDPIRVERARAVADTIARLVPLDDMMYCLEYG
                     AGTGLLSFALQPRVGGIVLADTSAGMLAVADAKIAAASLDEDLRVMKIDLLEDPLPED
                     RFNLLFTLLTLHHIPDIPAILDKFYEMLIAPGWLCIADLDLEDGSFHGEGFSGHRGFD
                     REALGKLVEAAGFVNIRCDTAYEIPRMAGGVLHHYPLFLLVAEKARR"
     gene            9748..10623
                     /locus_tag="PKM23_06085"
     CDS             9748..10623
                     /locus_tag="PKM23_06085"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91079.1"
                     /translation="MTTLILFLFLSGRSAAAEIELTGGWPSGPASAIAVQDSVVYLAH
                     GGFISAYHASTFAPLSQIALSGYIRSIACSGRYAYIAAMEAGLFILDIANPRQIKLVA
                     TVNDVNVLKAVVRDSWAYLATNVGGLRILDISTPSDPKVLTDFKPERWIQDVAVEGRY
                     AYLACNSAGLCVVDVQNPAAPAKLLSYPGGYVVYHVAVSGGKVFMCGSDDVRVVDLTN
                     INQPREITTLSGMVTSIVFQGSIAYLVNDTHANVELHDLTDAAIKRWAAAEGCMPPPS
                     RCSLASPMWRWKTRV"
     gene            10557..>11534
                     /locus_tag="PKM23_06090"
     CDS             10557..>11534
                     /locus_tag="PKM23_06090"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91080.1"
                     /translation="MYATAVAVQPGLAYVALENSGLTTYNISDPAKPVAYGKWQSDKT
                     LMPPFMVGDLMAFRVFGEGALRFYDVQDLLHPRLVTTAKLYMDAYFYNAPYLYYTDDA
                     MLRVCDLSDIYNPVEKSHLTLTSKTSAMRRQGDTLFIYDTMSNENGFALYSLADPQKP
                     VKLADVVTTTPVYVVGKKGPAVYLYARPNPSANESSLYCLDMTDPGAPKESAVMIPAP
                     IVRSFGIHDQYGIAATSEDSVIVFDLSRPLQPKRLAAFYDEEYAFPDFEGNLAYSSGR
                     MGVTVYDVSDMTRWRILSRLHEVDYYTGSFYYPPYFIKPTPEELRQHYLR"
BASE COUNT         2455 a         3351 c         3156 g         2572 t
ORIGIN      
        1 gattctgatt attgtctgga tttagggtgt actttttctc cagcctgtgg aggactggta
       61 ttgtcccagc tgaatccgca tttatgcagc ctgaacatac attatagaac gaggtttcca
      121 gatgagaaaa aagatgaccc tgctgttgct cctcggcctg ctcctcccgg gccgggcgca
      181 gaccctgaag gaggctttcg tcgacaccct ggtcgagcgc accctccaga ccttcaacgt
      241 ccccggtatc gccgtggccg tggtcaagga cgaggaggtg gtcctcgcca aaggctacgg
      301 ggtgcgctcc atccttaccc gtcagcccgt ggatgaaaac accctcttcg ccatcgcctc
      361 caataccaag gccttcaccg ccgccgccct tggcatcctc gtcgacgagg aaaagctgac
      421 ctgggataca cacgttaccg atttcatccc cgaattccgc cttcatgatc cctgggtcac
      481 ggccgagttc accatccgcg acctgctcac ccaccgcagc ggcctcggcc tcggcgccgg
      541 cgacctcatg ctctttcccg actcagcgcg cttcaccctc ccggaaatca tccacaacct
      601 tcgctatctc aagccggtct cctcgttccg gaccaaatac gactatgata atctccttta
      661 tctggtcgcc ggcgaagtgg tcgctcgcgc ctcaggcatg agctgggaag agttcgtcga
      721 gacacggatc atgaagcctc tgggaatgac ccattccgcc gcatcgatcg accgtctgcc
      781 cagcgccgcc aatatcatcg atgcccatgt catggtcgac ggccggcttc aggtcgttcc
      841 cctggagcgg ggcagtgtca tcaatgccgc cggggggatt tacagcactg tttctgacat
      901 ggccgaatgg gtgcaactgc agatcaacaa gggggcctat gacgaggcgc caggaaaaag
      961 gatttttagc gagcaggtcc actccgaaat gtggcagccc cagaccatcc tttcggtgcg
     1021 tcaacccgga tcctacaaca cccactttgc cgcctacggc ttgggttggg gagttgccga
     1081 tatgaagggt ttcctggaag ccagccacac cggtggcctg atggggatcg tcacaaaggt
     1141 caccattctg cccgaactta aacttggcat catcgtcttc accaaccagc aggagggagc
     1201 tgcctttgca gccatcacca acaccatcaa ggatttttat ctcggtatgg aatttaccga
     1261 ccgtgtcaag gaactgagcg aaagacgcca gcagcagctt ggcgaagcca gcgggattat
     1321 ggcggcagtg gagaaaaaga tcgcggagca gcggaaaaag aaggggggag tgcccgaccg
     1381 cgtcccctat gtaggcacct acacagacaa gtggttcggg gaggtcgcga tcaccctgga
     1441 gggagagcgg atgcgtttcc gggcgctgcg ctcgcccaaa ctgcgcgggg agatgtttta
     1501 ttacgccggc aataccttca tcgtacgctg ggatgaccgc accctcgagg ccgacgcttt
     1561 cgcctccttc accctcgatg aggagggggt gccgaccggc atgaccatga aggcggtttc
     1621 gcctttcacc gatttcagct tcgattttca ggacctggat tttattcgta aaacggagta
     1681 ggcagtccat atggcatgcc gcaacctgat caggcgccat ttcgctgaac agctcatccg
     1741 gcctcacggg ctggttggcc gctggatcat tgggccgctc tggaaccggc ggaatcgtgt
     1801 gctcaatgac aaaaccctgc aggcgctgcg gctgcgggag catgaacagg tgcttgagat
     1861 cggattcggt ggcggccgcc tgctggctca aatggccaaa attgtcacgc atggccacct
     1921 ctccggcctc gatgcctccc caataatggt aaatcgcgtc cggagaaaat tccaggcgct
     1981 tgtctataaa ggaattatgg atatccggct cggaagcgcg gaaaaactgc cttttaccga
     2041 cagcgctttc gacaaggtgg cctcggtgaa tgcgatcttt tactggcgcg atccagccag
     2101 agggattcgc gaagcctgcc gcgtgctgcg ccctgccggg ctgttcgtgc tgaccttcac
     2161 ggccaaggaa gatctggatc ggcaggagtt cggtattccc tcgttttacg aggatgaggt
     2221 gatcagtatg cttcgcaagg caggatttta ccggatcgat gtcagccgcg accgcgatgc
     2281 ccatcgagca tttttaatcc tgacagctga gaaatgagtg gaggaagaca atgcagcgtg
     2341 ttcttatcat attgggaatc ctgaccgcca ccctgtcggg ctgcacgacg aaacagccgg
     2401 aactgggtgc cgaggccctg cgccagcagg tgatcgcaac cgagcaggcg tttgcggcga
     2461 cgatggctga ccgcaacctt gcgggcttta tttcatttct atccgaggag accgtctttt
     2521 tctccggccc gaagcccctg cgcggggcaa aggcggtagc cgaatggtgg cagaaatatt
     2581 atcagggcgc cagtgcgcct ttctcctggg cgcccgaaca ggtggaggtg ctcgactctg
     2641 gttccctcgc cctgagcagt ggaccggtgt acgatcggga gggcaaactg accggcacct
     2701 ttacctcgat ctggcggctg gagtccccgg gaaaatggcg cattattttc gacaagggct
     2761 gtccggtttg cgactgatcc cccggcgcaa tatataaaaa ggccaggaag agcaaatcct
     2821 cctggcctcg tttttatctg caggccggca atcggcggct gtatcatccc tggtcagacg
     2881 ctttgcggga ggtcagccga tcggggcacg aatatccaca gcagaatata ggcgagcact
     2941 ccaaaacagt aaaagaaggc aagagtcaca aagatcaccc gccaggtcca ggagggcaca
     3001 tcactgtgct ccccgagtcc gccgcagatg ccggctattc tgcgatcctt ttcagaacgt
     3061 cggaactcgc tcagccaact gttgcgaggc gcttgtgccg gaccggaggc cgaggccggg
     3121 ggcatgaaaa tccacatcag gatatagagc agcgcaccgg tgccgaaaca gataaaggca
     3181 aagacaaata aaagccgcca ggtccaggcc ggcacaccgg tgtgctcacc caatccgccg
     3241 caaaccccgg cgatcacttt gtcgctgctg gatctcgcca aacgctgaag catattctgt
     3301 tccatggcat ctcctccctg gttgtacacc tcttgatacg ccgctttttc caaaaagatt
     3361 cattccccac ccacgccaca gccgattttg gcatgaaaac gaaacatctt gcttcaaaac
     3421 ttcgcgttgg ctatctttat cagtaagaca gctgtttcaa aacataaaga accggagggc
     3481 atcttggaac cagccaaaat cgtatcacgc atccgcgccg cagcttgagg gtgattcgaa
     3541 ctgaatgtgc cattcgctga atctgccgca atggaaggaa agtcatgacc ccaaaacgca
     3601 aactgaagaa tccggaggat ccgcaaacca tccccggcgt cggtcctaat atcgccgcac
     3661 actttggcga gctgggtatc catcgcgtct cccagctccg cgatgcggat ccggagggga
     3721 tgtacacccg gctctgtgaa ctccacggcg gccccatcga ccgctgcctg ctctatgttt
     3781 tccgtgaagc ggtctattac gcaagccacg accgccatga ccccgaactg ctcaaatggt
     3841 ggaactggaa agagggcgcc gcagcctata ccaaaaatta aggggcggcc gctgaaggct
     3901 gcgccgtttt ctccgcttgt aaaattttcc ggaattactc cccgtcatgc cgtcgcggcg
     3961 catagctcgc ctccggatca atccgctccg cgtagggact ctcccagctc tggttgaaaa
     4021 tctgcagcag ccgtccggcc aggggggtgc tgcgcacaat caccccggca ttgcgcgaag
     4081 tgtgaaaata gctccgcgcg gcattgctgg tgcccagcca aaaggcctcc ccatccacga
     4141 ggatatactt gcagtgttcg acccgggcgt aggagatata gcccccggac cactccggga
     4201 tgacactgaa gcagacctcg atgttgggca gctgcgccag ctccttgagg gcggcttcgg
     4261 ccctgcttcc cttctgccag tcggccacca gcaggcgcac tcgcacattg cgggctgccg
     4321 cccggcgcag cgcgccgtcg aaggggtcct cggcgccgcc gtttctcgat cggggtgaat
     4381 aggaaaggaa ttgcagggcg atctcgcgct gcgccccgtc gagcaggcgc acgatctgct
     4441 cctcacacca gagtgtggtg tctgggatca acgcccgcgg gctgaaggtg ggctgaatga
     4501 tggccgggcc atactcagag gtcaggagtc taatgggtgt ttcatagagg cgcggctgga
     4561 ttaccgggcg gctggtgtcg gccggcgtcc cgtccgcgag acgccagtcg agttcgaaca
     4621 gctcctggta gatggagacg acctgcgtgc tccggatccg tatccccagc tcgtggatat
     4681 gggtgagcgc ccgccagtcg aaattctggc tgccaagaaa gaggctctcg ccgtcgatga
     4741 ccatgtattt ggcgtgctgg atcccgccgg ccagggttcc aaaatcgatg aggcgagcgg
     4801 tgatgttttc ccacttccca agagaatcga tcagcaccgg ataggtttta tacatccggg
     4861 catctgccag gatacgcacc ttcaccccgc gccgggccgc cgcctgaaga gcggtgatga
     4921 cctgatccag actctcacct gcttggtggg agagatagaa ctgctccaga tcgatggtgc
     4981 tgtgcgcgcc ctcgatgagt tcgagccaga cctccagggt gttgcggatg tcggggttgt
     5041 cgagacaggt ctccaccggg atgctctcga ccagctcgat ctccgccggg gtttgcgccg
     5101 cgagcagggg ggagaggatg aaaaggaaga ggagggagga aaagaggatg cgcttcatag
     5161 ggtgatccat ttctgcttgt ggtccgctct acccagtccg tgcggcccga ctttacgccc
     5221 ggcagccgct ttcaggttga ctttttccgt gcgaatgatt aatatctttt gtgcatcacc
     5281 ctctgtaaca tttaatccag gccttgaaat ttcaattatc caaatagtgt ccggtgacac
     5341 ggcccgccac agcacacagg ccggctgcct gtttttagat gacagaatca gaatccttta
     5401 atcgcttcct gcacgacttg gtcagcacgc ccaccgattg aggagaatat gacattccac
     5461 aaatttttca cctggttaat tctgttttct gccgcattcc tcttttcagc aaagagtcac
     5521 acagaggaac agaaatggct gactgtctat gaaaaatccg ggggtctgga aaccccgcgc
     5581 tacgccgaaa ccatggccta ctttgagaga ttggaccagg cctctccctt cgcccggctg
     5641 gaatcgtttg gaaaaagccc ggaggggcgt tcgctgcccc tcttcatcat ctcctcggac
     5701 aaggctttca ctccggaaat ggcgcgccac accaacaagc cggtcgtcct cattcagggc
     5761 gccattcaca gcggcgaatc agagggcaag gatgcggtga tgctgctggc gcgtgacttg
     5821 ctgatctata aaaagcatcg tgagctattc gaaaatttta ttatcctcat catccccatc
     5881 ttcaacgtcg atggccacga acgcttctcg gcctacaacc gcatcaatca gaacggtccg
     5941 caggagatgg gctggcgggt gacctccaca cgcctcaatc tcaaccgcga ctttatgaaa
     6001 gccgatgccc cggagatgcg ccagtggctg cagatgtacc atcgctggtt gccccacctc
     6061 ttttacgact gccacaccac cgacggtctg gatttccagt atctcctcac ctggaatatc
     6121 gatgaacacg agcagttcgg cggcgccgtt tcccggtgga cgagggatac ctttctgccc
     6181 ggcgtcggga aggcgtgcga aacgcagggt ctgctcatcg ccccctacgc cgacatgctc
     6241 gaagatgaac ggcctgaaaa aggctttgtc atcggcgtct ggcggccgat gctctccact
     6301 acctacgcca cggtctgcaa ccgcgccgga ttcctcatcg agacccattc gctcaagccc
     6361 tacggcaccc gcgttcaggc caccctcgat ttcatcctta tcggattgca ggagatcgtc
     6421 aaaaatccgc atgccttgct tcaagctgta caagaggaag atcagcgcgt ctccgcctgg
     6481 ggccgcgtgc cggcggatac ccttaaagtc cccctgcaat tcagaacgcg cttcgaccgc
     6541 ggagagagcg ctctctttta cggctatcgg gtggagataa aaccaggcgc cgtttccgga
     6601 cgcgactatc cggtctatac atcggataaa gtggagatac ccacggtttt ctataatgag
     6661 gttgaaccaa ctgcagaggt ggcgccgccc cgcggctatc ttgtcccgtc agggtggcag
     6721 gccatcatcg atatcctgcg ccttcatggc atcagcatgt atcgtctgaa agaggagtac
     6781 agaggtttat tcgagggcta tcgcttcagg gaggtccgct tcatgccgcg ctcctatgaa
     6841 ggccgctcta tcccttccta ccgggtcgag gcagtgaccg aaaagcgcgt ttttcctgaa
     6901 ggctcggtct atgtgccgct aggcaatccc cgcgccaaac tgatcatgca tctgcttgag
     6961 cccgaggccc cggacgccct ggtggcctgg ggcttgatga acaccatctt tgaggaccgg
     7021 gaatactttg agtcttatgt gatggaaccc ctggcacaaa agatgatggt ggacgatccg
     7081 gggctggcag cgctcttcac gcaacgagtg cactcggaca gcgcctttgc cgccaacccg
     7141 cgggcgcggc tgggcttttt ctatcagcgc tcgccctggt acgatcagga taaggatcgc
     7201 tatcccgttt ggcgcgtgac cggtgatcgg ccgccggcgc tggaagagct tcagtaacga
     7261 gtgtggatgt ccagtatcat ggtcaatgag ggaaggatca gccgggcaga acagaagcga
     7321 ttcaggatgg gatgcaaacc gggtctgtgc ccgcagtgag gtccccttat gccggcggaa
     7381 aaatgactgg ggcagcgcgg cgaaaattga ccaggtcaaa ggtatctatt ttttgttcaa
     7441 tctggccatc ataaacgaga tatccctctt tgaaccgctc aggcatgatt ttttgcagat
     7501 agcgcagccc tttcagaaaa tcagcgtgaa atgtttgcgc tgattttatc tcgacaggca
     7561 acagatccga tccccgtttg aagaggatat cgatttcatt ttgatggttg tcccgataaa
     7621 agtggaattg gggatccaga ccgcgattgg ttcgagtttt tattaactcc atcaacacca
     7681 tattttcaaa tagcgctccg cgcaaaggat ctctcgccag ttgttgtgta ctttcgatgc
     7741 ccagcaaata ggtcgccaaa ccgacatcat agaaatagag tttcgccgct ttgataagcc
     7801 ttttaccaaa attttcaaaa tagggaggta aaagcataac aatataggag gcctgcagaa
     7861 tggagagcca ggatttaatc gttactgcac tcgtccccac ttcattggca aggctgcttg
     7921 cgttgaaaac ctgtcccact cgtcctgcac acaacctgac aaagcgttga aactgcaata
     7981 aatcctttat gtggatgagt tgtcgcaagt ctctctctat ataggtctca tagtaacttc
     8041 ggtaggtaat cgaaggattc aagcggtcgc cgtaaatggc cggataaaat ccgtttaaaa
     8101 gatagtcatc cgtacttgct ccgccagttt gtttttcaag ctcattgatc gtgagcggca
     8161 gcaattttag caggaccgtt cgccccgcca gggattgtgt gaggctgttc agcagggcaa
     8221 actgattgct tcctgttaat atatactgcc ctttcagggg tcggctatcc accacttcct
     8281 gtaggtagga gaagaactcc ggaacgcgct gtacctcatc caggatggcg ccttgctgaa
     8341 tgcggttgag aaaggctcgt gggtcgctct gaacagtctc ccgcagatcc ggattttcca
     8401 ggctgaaata tggtttttcc ggaaataatt tttttactag tgtggtcttg ccggattgcc
     8461 tcgggccggt gatggttaca atagggtact gggctgccag caagacaact gttttttcaa
     8521 tttctcgtgt gatatacata ttttgtacag ataattactt tgactttatt attgtacaaa
     8581 tataaccctt ttcgaggtaa aaatcaaggg tgttttaatg atcagtatga attctgacac
     8641 cgctgctgcg acctctgccg ccagtgactc atctcgtgca gcggatgcgt cctggactgt
     8701 tcacgaagac acttgttttt cgtcgtgacg gcctgcattg gaaagtgaaa atcagaggga
     8761 aaaagatgaa cgattttgat gcccgcgccc gcacctggga cgatgacccg atccgcgtag
     8821 agcgcgcccg tgctgtcgcc gataccatcg cccgacttgt gcccctggat gacatgatgt
     8881 actgtcttga atacggcgcc ggaaccggat tgctcagctt cgccctccag cccagggtcg
     8941 gcggcatcgt cctcgccgac acctccgcgg gcatgctcgc ggtcgcggat gccaagatcg
     9001 ccgctgccag tctggacgag gatctgcgcg tcatgaagat cgatctgctc gaggatcctc
     9061 ttccggagga tcgtttcaac ctgctcttca ccctcctcac cctgcaccac atccccgaca
     9121 tcccggccat cctggataaa ttttatgaaa tgctgattgc gcccggctgg ctctgcatcg
     9181 ccgatctcga cctggaagac ggctcgtttc atggcgaagg tttttcaggc caccgcggct
     9241 tcgaccggga ggcgctcggc aagctggtcg aagcggcggg ctttgtcaat atccgctgcg
     9301 ataccgccta tgagatcccc cgcatggccg gcggtgtcct gcaccactac cctcttttcc
     9361 tcctggtggc ggaaaaggca aggcgttgac ccgcgccaga gctcctgtat ttgcctcgcc
     9421 cccctttcac cccgtatccg cgctgatccc ggcgggatat cgccagcgtg ctattctggt
     9481 atttttgctt gtatctcaaa tctcgattgt ttatttttta atcagggcat gggctgaaat
     9541 gcggctggcg ctccgagtat ggggaaagag ctgggtatca agcacctgac accagataat
     9601 ctcctcgatt tattttaccc ccggtaaaat cacaattctc aatagcacaa cgcgggcatg
     9661 acaacccgga ctggcactgt ggaacgcact cctaatcagg aaaggattcc tatggtctgg
     9721 aaatgcaagc agtgtatggt tctgccgatg acaacactca tcctgttttt gtttctctcc
     9781 gggagaagtg cggcggcgga aatcgagctc accggcggct ggcccagcgg accggcctcg
     9841 gccatcgcgg tccaggacag cgtggtctat cttgctcatg gcggtttcat ctcggcgtat
     9901 cacgccagca cttttgcccc gctctcacag atcgcgctct ccggctatat ccgcagcatc
     9961 gcctgctcgg gccggtatgc ctatatcgca gccatggaag ccggtctgtt catcctcgat
    10021 attgccaatc cccgccagat caaactggtc gcaacggtca atgatgtcaa tgtactgaaa
    10081 gccgtggtgc gggattcctg ggcctatctg gccaccaatg tcggcggctt gcggattctc
    10141 gacatcagca cgccctccga tccaaaggtc cttaccgatt tcaagcccga acgctggatt
    10201 caggatgtcg ccgtggaagg ccggtatgcc tatctggcct gcaattccgc tggcctctgc
    10261 gtcgtcgatg tgcagaatcc cgccgcgccg gccaagctgt tgagttaccc cggcggctat
    10321 gtcgtctatc acgtggccgt ctccggcggc aaggtcttta tgtgcggcag cgatgatgtc
    10381 agggtcgtcg acctgaccaa catcaaccag cccagggaga tcacgaccct gagcgggatg
    10441 gttacgagta tcgtctttca gggttcgatc gcctatctgg tcaatgatac ccacgctaat
    10501 gtggagctgc acgatctgac cgatgccgcg atcaagcggt gggcggctgc ggagggatgt
    10561 atgccaccgc cgtcgcggtg cagcctggcc tcgcctatgt ggcgttggaa aactcgggtt
    10621 tgactaccta taatatcagc gatccggcca agccggtagc atatgggaaa tggcagtccg
    10681 acaaaacgct gatgccgccc tttatggtgg gcgatctgat ggcttttcgg gttttcggcg
    10741 agggcgcctt gcgtttttat gatgtccagg atctgctgca cccccgcctg gtcaccaccg
    10801 ccaagctcta tatggatgcc tatttctata atgcccctta tctctactat accgacgacg
    10861 ccatgttgcg cgtctgtgat ctgagcgata tctataatcc ggtagagaaa agccacctca
    10921 ccctgacctc gaaaacctcc gccatgcgcc gccagggcga caccctcttc atctatgaca
    10981 ccatgagcaa tgagaatgga tttgcgctgt acagcctggc cgatccccaa aagccggtca
    11041 agctggctga cgttgtcact acaacacccg tttatgtggt gggaaaaaaa ggaccggcgg
    11101 tctatttgta cgcccggccg aacccctccg ccaatgagag ctcgctctat tgtctggata
    11161 tgaccgaccc tggcgcgccg aaagagagcg ccgttatgat cccagcgccg atcgttcgct
    11221 cctttggcat ccacgaccag tacggcattg cggcaacctc ggaggacagc gtgatcgtct
    11281 ttgatctcag ccgccctctc cagcccaaac gactcgccgc cttttacgat gaagagtatg
    11341 catttcctga ctttgagggt aatctggcct attcctccgg acggatgggt gtgactgtct
    11401 atgatgtttc cgacatgacc cgctggcgca tactcagccg gcttcacgag gtcgattatt
    11461 atacgggctc cttctactat ccgccctatt tcatcaaacc gacgcccgaa gagctgcgcc
    11521 agcattattt acgc
//
LOCUS       DAOPWU010000083        11531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5701_length_11531_cov_5.124259, whole genome shotgun sequence.
ACCESSION   DAOPWU010000083 DAOPWU010000000
VERSION     DAOPWU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5701_length_11531_cov_5.124259"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKM23_06095"
     CDS             <1..638
                     /locus_tag="PKM23_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91081.1"
                     /translation="LDPAESASILFLVRGIDHYFFDIDLRYRATHISAGRFPEAGMVG
                     NLPSGEAYIVPYEGENEPPSRTRGELPVQFGGEIVLYHVEKNRARSAFGSGPAARVEN
                     ERLLAEPAYGNIAELGFGVLADFGVEPVGEILLDEKLGLHIAFGRSDHFGGFVGAGQF
                     SSPQALVHIDRIYIPQTQPLIEVASVKLHYPDGREDEVIRSGRYLDFTPTA"
     gene            1060..1797
                     /locus_tag="PKM23_06100"
     CDS             1060..1797
                     /locus_tag="PKM23_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91082.1"
                     /translation="MRDTLAALINLQDVDCELRALEQSKGDLPQRIASLNREIAQLEE
                     SIAEKHDQLKTSRLGRESGALEVASLRDKLKKYQNQLYQVKTNKEYDAITLEIETTQS
                     AIENREFEMLEMEEREKQLHSEIEGLQPGLAELQGQRDEAESRLQAMLAVTRAKEERL
                     QQQRQEIVARIPRPVYSTYERTRIGRGGIAVAFLKEGSCSQCSTRIPPQRGLEIRMMN
                     HLFLCEVCGRIMLYDPERMTVCEGHQQ"
     gene            1813..2185
                     /gene="rnpB"
                     /locus_tag="PKM23_06105"
     ncRNA           1813..2185
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKM23_06105"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2273..3970
                     /gene="recJ"
                     /locus_tag="PKM23_06110"
     CDS             2273..3970
                     /gene="recJ"
                     /locus_tag="PKM23_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNY91083.1"
                     /translation="MELRWDVLDQVSADEVNKLSQSLRIPPVIARMLLCRGITEVDTA
                     RRFFEPSLDLLYDPFILDDMQRGVDRLRRAVLSDEKILIYGDYDVDGITSVSFLYLIL
                     KELGAQVGYYIPDRQSEGYGLSIAGIEAAQKSGTNLIITVDCGITGHLEIDAARNLGI
                     DVIVCDHHEPGETLPDAYAVIDPKVGDSPYPFRELAGVGVTYKLCQGLLLRMEIDLSI
                     LENYLELVAIGTAADIVPLVDENRVFVKEGIERLNESENIGIKALLQVAGLGGKEVST
                     GHIVFIVAPRINAVGRMGDAERAVKLLTTEDPAEAAEIANVLEMENRHRKNVDEETFR
                     QALTIAEEQYAEGRARSLILHQRGWHPGVIGIVASRVVERFYRPTVLISVDNGVGKGS
                     ARSIEGFDLHDALKQCEDLLIGFGGHKYAAGLSIAEENIPAFHERFEKVAAGRLVPEQ
                     LIPKLTIDAELRLHEIDALFLKLLRMFAPFGPLNTRPVFMSRNLQVLGTPTIVGGNHL
                     KFKVRDAGAAFEVIGFGMGHYLPRVQQAESGLDLVYVIEENEYNGRKTIQLRAKDLR"
     gene            4014..4940
                     /locus_tag="PKM23_06115"
     CDS             4014..4940
                     /locus_tag="PKM23_06115"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNY91084.1"
                     /translation="MDARDEKSKIWMNGRFIPWQEATIHVMSHVVHYGSSVFEGLRCY
                     DTPKGPMIFRLREHIRRLVDSAKIYRMTVPYSVEELVQACNDLIKINQFPAAYLRPIV
                     YRGYHSLGVDPRKCPVDVAIGGLNWGKYLGDEALAAGVDVRVSSWQRMAPNTLPAMAK
                     AAGNYMNSALIKQEALADHYVEGIALDVNGQVSEGSGENLFVVRDGVVYTPPVSASIL
                     SGITRDSVIKLCAEMALPVKETVLPRELLYIADEVFFTGSAAEITPIRSIDHVVIGSG
                     KCGPVAAELQTRFFAYINGEREDNYGWNHYVR"
     gene            4915..5454
                     /gene="nusB"
                     /locus_tag="PKM23_06120"
     CDS             4915..5454
                     /gene="nusB"
                     /locus_tag="PKM23_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNY91085.1"
                     /translation="MAGTTTSDNHNLEDEDVYHASAPGSRRRGREIALQGLYALELSG
                     DSPAKVLRDVLSFHEEESIIRDFARDMVTRAAEHHDELDGHIRRHVTNWDFDRIAIID
                     RIILRMAICEFLYFFDIPPKVSIDEAIEMSKLYSTDQSGRFVNGILDAVLLDLKKSGQ
                     LVKSGRGLQNASSGEKQET"
     gene            5580..9983
                     /locus_tag="PKM23_06125"
                     /pseudo
     CDS             5580..9983
                     /locus_tag="PKM23_06125"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10812..>11531
                     /locus_tag="PKM23_06130"
     CDS             10812..>11531
                     /locus_tag="PKM23_06130"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91086.1"
                     /translation="MIKSVTAWEGTTESKQRLLSARLEFILQQRPPKSLPEHGDEALV
                     ASYAEDVVAWALKWYATYAERQERLHQETLSSEIVRDSVLTMIQDTVYTMISNLLSGD
                     SGLDAAAIRRLESDCRLVFRQAPRLSDDSMEGYDPNAIMEQVTRWAVALYQKRIAELG
                     RERATRYERYFVLEKIDENWRQHLNATDELREGIGLRGYGQKDPLLEYKREAFELFVK
                     MNERVNRDVVATLFKVFDVGGE"
BASE COUNT         2326 a         3746 c         3402 g         2057 t
ORIGIN      
        1 aactggatcc ggctgaatcc gccagcatcc ttttcctggt ccgcggcatc gaccactact
       61 tttttgacat cgatctgcgc tatcgcgcca cccacatcag cgcaggccga tttcccgagg
      121 cgggcatggt cggcaatctc cccagcggcg aggcctacat cgtcccctac gagggggaga
      181 atgagccccc gagccgcacc cgcggcgaac ttccggtcca gttcggcggc gagatcgtcc
      241 tctaccatgt ggagaagaac cgcgcccgca gcgccttcgg ctccggcccg gccgcccggg
      301 tcgagaacga gcggctcctg gccgaaccgg cctatggcaa tatcgccgag ctgggattcg
      361 gagtgctcgc tgactttggg gtcgaaccgg tcggtgagat cctcctcgac gagaagctgg
      421 ggctgcatat cgccttcggc cgcagcgacc attttggggg attcgtcgga gcgggccagt
      481 tttcttcgcc ccaggccctg gtccacatcg accgcatcta catcccccag acccagcccc
      541 tgatcgaggt cgcctcggtc aagctgcact atcccgacgg ccgcgaggat gaggtcatcc
      601 gcagcgggcg ctatctcgat ttcacaccca cggcctgatt ttttcatcat ctccccgctt
      661 ccggcaagtt ttctcttgca ttttagccta taaagctgta cttttgatta ctttttgaat
      721 aaaccgtttc cccacccctg caatcgcact tgccttttgc gtgatcaacc gccacaagat
      781 gcctcattcg ggattctgaa gcgaagcagc tccgttttat cgattacagc aaaagtgtac
      841 agttccagga ttgcaggagg gagacttgac ctctagttct ttgcagccat gaagttggtc
      901 gtgcgcgtga taaatacccc ccttgtgaaa tcgcccgaaa aagaaggcct tgcggcgccg
      961 cgcgccagcc ggttctgatt tgaaagtgca tcccgtccag gtgcactttt tttatgtata
     1021 ttattccggc cgccatccgg aaagacagga gaaaaaacct tgagagatac cctggcagct
     1081 ttaatcaatt tgcaggatgt cgattgcgaa ctgcgcgccc tggaacagtc caagggcgat
     1141 ctcccccaac gcatcgccag cctcaaccgg gagattgccc agctggaaga gagcatcgcg
     1201 gagaagcacg accagctcaa gaccagccgg ctcggacgcg agagcggggc cctggaggtg
     1261 gcctccctgc gcgacaagct gaaaaaatac cagaaccagc tctatcaggt caagaccaac
     1321 aaggaatatg acgccattac cctcgagatt gagaccaccc agtccgccat cgagaaccgc
     1381 gaattcgaga tgctggagat ggaggagagg gaaaagcagc tgcacagcga gatcgagggt
     1441 ctgcagcccg ggctggccga gctgcagggg cagcgggatg aggccgagag ccgattgcag
     1501 gccatgctgg cggtgacgcg ggcgaaagag gagaggctgc agcagcagcg gcaggaaatt
     1561 gtcgcccgca tccccagacc ggtctacagt acctacgaac ggacccgcat cggtcgcggc
     1621 ggcatcgccg tcgcctttct caaggagggt tcgtgcagcc aatgctccac ccgcatcccg
     1681 ccccagcgcg gcctcgagat ccgcatgatg aaccacctct tcctctgcga ggtgtgcggg
     1741 cgcatcatgc tctacgaccc cgagcgcatg acggtttgcg aagggcatca gcagtaagcg
     1801 agtttttgct ccaaagcagg acaggtgatc gcgctccttt ttgccatgca ggagggggtg
     1861 aggaaagtcc gaactccgca gagcagggtg gtccgtaacg cggacccgtc gtgaggcggg
     1921 gaaagtgcca cagaaagcag acatccccgg ctcaggccgg ggttaaggtg aaagggcgag
     1981 gtaagagctc accgtcccga tggcgacatc gggagcaggg taaaccccac ccggagcaag
     2041 gccaaataag agggaagagg ctgctcgcct cgcttgaccc tcgggtaggc tgctcgaggt
     2101 gcggagcaat ccgcatccca gatagatgat caccaccccc gcccaggcgg ggggacagaa
     2161 ttcggcttat cgtcccgctt tggagcattt ttatttcgtg accggagggt tcctcttccg
     2221 gtcctcaatc atggtggcaa gtcaccggat aattggatgg agacaggggt ttatggaact
     2281 caggtgggac gtcctggatc aggtttcagc ggatgaggtg aacaagctct cgcagtcgct
     2341 gcgcattcct cctgtcatcg ccagaatgct gctctgccgg ggcatcaccg aggtcgatac
     2401 ggcgcgccgc tttttcgaac cctcccttga tctgctctac gatcccttca ttctggatga
     2461 tatgcagcgc ggcgtcgacc ggctgcgcag ggctgtcctc tccgacgaaa agatcctcat
     2521 ctacggggat tacgatgtcg atggcatcac ctcggtctcc ttcctctatc tgatcctcaa
     2581 ggagctggga gcgcaggtcg gctattacat cccggaccgg cagagcgagg gctacggcct
     2641 ttcgatcgct ggcatcgagg cggcgcaaaa gtcggggacc aacctgatca tcaccgtcga
     2701 ctgcggcatc accggccatc ttgagatcga tgccgcacgc aatctgggca tcgatgtcat
     2761 cgtctgcgac catcacgagc cgggggagac ccttcccgat gcctatgctg tcatcgatcc
     2821 caaggtgggg gattcgccct atcccttccg tgaactcgcc ggggtcggtg tgacctacaa
     2881 gctctgtcag gggctgctgc tgcgcatgga gatcgacctc tccatcctcg aaaactatct
     2941 cgagctggtg gccatcggca cagccgcgga cattgtcccc ctggtcgacg aaaaccgcgt
     3001 tttcgtcaag gaggggatcg aacgcctcaa cgagagcgag aacatcggca tcaaggccct
     3061 gctccaggtg gcggggctcg gcggcaagga ggtcagcacc ggccatatcg tcttcatcgt
     3121 cgcgccgagg atcaatgccg tcggccgtat gggggatgcc gagcgggcag tcaagctgct
     3181 cacgactgag gaccccgccg aggcggccga aatcgccaat gtcctggaaa tggaaaaccg
     3241 tcaccgcaag aatgtcgacg aggagacctt ccgccaggcc ctcaccatcg ctgaggaaca
     3301 gtacgcggag ggccgcgccc gctcccttat tctccatcaa cgcggctggc atccgggggt
     3361 gatcggcatc gttgcctcgc gggtggtcga acgcttttac cggccgacgg tgctgatctc
     3421 ggtcgacaac ggcgtcggca agggctcggc gcgcagcatc gaggggtttg atctgcatga
     3481 cgccctcaag cagtgcgagg atctgctcat cggcttcggg gggcataaat atgccgccgg
     3541 gctctccatc gccgaggaga acattccggc ctttcacgag cgcttcgaga aagtggcggc
     3601 cggccggctt gtgccggagc agctcatccc caagctgacc atcgacgcgg agcttcgcct
     3661 gcacgaaatc gacgccctct ttctcaaact gctgcgcatg ttcgccccct tcgggccgct
     3721 caacacgcgc ccggttttca tgagccgcaa tcttcaggtc ctgggcaccc ccactatcgt
     3781 cggcggcaac catctcaaat tcaaggtccg cgacgccggg gccgctttcg aggtgatcgg
     3841 cttcggcatg ggccactacc tgcccagggt gcagcaggcg gagagcggcc tggacctggt
     3901 ctacgtcatc gaagagaatg aatacaacgg ccgcaagacg atacagcttc gcgccaagga
     3961 tctgcgctga gcagccgtct ggcatatcgc gaacccgaaa agaggagtag gtgatggatg
     4021 caagagatga aaagagcaag atctggatga acggccgctt catcccctgg caggaggcca
     4081 caattcatgt catgtcccat gtcgtccatt acggttccag cgtttttgaa gggctgcgct
     4141 gttacgacac gcccaagggg ccgatgattt tccgcctgcg ggagcatatc cgccggcttg
     4201 tcgattccgc caagatttac cgcatgaccg tgccctactc cgtggaggag ctggtgcagg
     4261 cttgcaacga cctcattaaa atcaaccagt tccctgccgc ctatctgcgg ccgatcgtct
     4321 accgcggcta tcactccctc ggcgttgatc cgcgcaaatg cccggtcgat gtcgccattg
     4381 gcggcctcaa ctggggcaag tatctcggcg acgaggccct cgccgcgggc gtggatgtgc
     4441 gcgtctcctc ctggcagcgc atggccccca acaccctgcc ggccatggcc aaggcggccg
     4501 gcaattatat gaattcggct ctgatcaagc aggaggccct cgccgaccat tacgtcgagg
     4561 gcatcgccct ggatgtcaat ggccaggtga gcgagggcag cggcgagaat ctttttgtcg
     4621 tccgtgacgg cgtcgtctat accccgccgg tttcggccag catcctcagc ggcatcaccc
     4681 gcgacagcgt gatcaagctc tgcgccgaaa tggctttgcc ggtcaaggag accgtcctgc
     4741 cgcgcgagct gctctatatc gccgacgagg tctttttcac cggctcggcc gcggagatca
     4801 ccccgattcg ctccatagat cacgtcgtca tcggcagcgg caagtgcggc ccggtggcgg
     4861 ctgagcttca gacacgcttt ttcgcctata tcaatggcga gcgggaagat aactatggct
     4921 ggaaccacta cgtcagataa ccacaacctc gaggacgagg acgtctatca cgcctcggcg
     4981 ccgggctccc gccgtcgcgg ccgtgaaatc gccctgcagg ggctctatgc ccttgagctc
     5041 tccggcgatt ccccggccaa ggtgctgcgc gacgtcctca gcttccacga ggaggagagc
     5101 atcatccgcg atttcgcccg cgatatggtg accagggcgg ccgaacatca tgatgaactc
     5161 gacggccata tccgccgtca tgtcaccaac tgggatttcg accgcatcgc catcatcgac
     5221 cgcattatcc tgcgcatggc gatctgcgaa tttctctatt tttttgacat accgcccaag
     5281 gtctccatcg acgaggcgat cgagatgtcc aaactttaca gcaccgacca gagtggacgc
     5341 ttcgtcaacg gcatcctcga cgcggtgctg ctcgatctca agaagagcgg tcagctcgtc
     5401 aagagcggac gcggccttca aaacgccagc agcggagaaa aacaggaaac ataaacgccc
     5461 cttcggacgt tttaatattc agcccattcg gttgccgctc gcactccggg cggcgccttt
     5521 tttttagcac ctgccgccgt tcacccggct tgaattttcc gaacagagga atgctgcaca
     5581 tgagttcaat taccgatatt ttttccaaac tggtcggcgg gaccagcaat gagaagcagg
     5641 tccggcgcct caacccggtc gtcgatcagg tcaacgcctt cgccgccgag tacgaaaaac
     5701 tcagcgatga cgaactcaag gccaagaccc atgagttcaa gtaccggctc aataccggcg
     5761 aaaccctgga tgacatcctc cccgaagcct acggcgtggt caaggagacc tgccgccgcc
     5821 tggtcggcca gaaatggctg gtgcggggcc aggagatcac ctggaacatg gtcccctacg
     5881 acgtgcagat cctcggcgct gccgtcctcc atcagggcaa gatcgccgag atggccaccg
     5941 gcgagggcaa gaccctggtc gccaccatgc ccctctatct caatgccctc accggcaagg
     6001 gggcccatct ggtgacggtc aacgactatc tggcccagcg cgactgtgaa tggatgggca
     6061 aaatctactc ctttctcggc ctcaccaccg ccgccctgca tggcgagatg acccccgaag
     6121 aacggcgtga tgcctacaat gccgatataa cctacggcac caacaacgag ttcggcttcg
     6181 actatctgcg cgacaacatg gctgctgatg tctggaatgt cgttcagcgc aggctcaact
     6241 atgccatcgt cgacgaagtc gacagcgtcc tcatcgacga atcccgcacc ccgctgatca
     6301 tctccggctc ggtcggggcg ccccgcaata tctattacga actcaagccg atcgtcgcca
     6361 acctctaccg ccgccagatg gacctggtcg ccgagctgct gcgcaagggc aaggccctgc
     6421 tcgaaagcga ccaggatgag gcgggcatgg ccctgctgcg cgtccagcgc ggcgacccca
     6481 agaacagtca actcctcgaa ctgctcacct ccgaattctg ggtcaaaaag ctgatcgagg
     6541 ggatgcaggg acagtacgag atcaacaagg agatggtggt cgtcgatgcg gagctctatt
     6601 acaccatcga cgaaaagagc catgtcgtcg acatcaccga gaagggacgc atcttcctct
     6661 ccggcgggcg cgatcaggac atcgtgcgca agattggcat gctcgacgcc ctcgacgaag
     6721 agctgggcaa gctggctcag cagaagaatc cccagcgcta tttctccata gatcccgtca
     6781 gcggcctctg caacggcatc accctcgccg gccaggtggc cctgaccggc gcctcgcacc
     6841 cccttgaagc ggcggtggaa gctgccgcgc agagactcgc cggacgcatc gccgccctgg
     6901 cgcagaccgc ggccgaacta gcccagcagc agaaagtgga caaggcggcc gcctggcggc
     6961 agctctatct ctactccaaa aagaccgaac gtttggcggt cggactcacc gacaagggca
     7021 aagccctgct cggcgaggga gagagcgatc cggccttcac aggtctactc tccgcctacg
     7081 accagctcct caatcagctc gagcctgaga gcgccgagga ccgcctcgaa gtggaacgcc
     7141 gccgcgaatt cgccggccag cttttcgaac tggacaagaa cagcaatgcc ccggtcgccc
     7201 tgcaccccga ggggcagata gccctgatcg ccctgcacct gcagggtgat ccccgcggcg
     7261 tctctttcat cgtcaagatg aacggactgc tgcgcggcgc cgatcccatg gacgtcaaac
     7321 ggcaggagta ttttgaattc tccgacaagg gaggcattcc gcgccatgtc gccgaaaagg
     7381 ggcgcatcgc cctgctcggc ggcaatcccg atctctacat tcttcccgac cgcaccctcg
     7441 tcgaggagcg cgaccgccac attcaggagc tgctcgaccg caccctgaac cagaacacct
     7501 ttgattatac cgagcgcgtc ctcgccatcg aagcccttga aagcgatctc gcccagatct
     7561 gcgccctcaa cagcccggac catcccttca tcacgccggg ggaggagggc gctcgccttc
     7621 agttcacccc agccggagcg gccctcctgg ctgactacgc ggagcagacc cgggggatga
     7681 tcgccctctt cgaccgcgat ctgcgcggtg agaaacacca gtccgggacc ctttttgcgc
     7741 cggacagccg gggcggctat gccggcctcg cagccgccga gcgcgaccgc ctgctcggag
     7801 caccatacga ggccgtgcgc agccgcatcc aggagtggcg cgcccggcag accggcgccg
     7861 acgccacccc cctcgccctg cgccgggatc tcgacgcctt tctcggtgcc tctttccccg
     7921 aggcgggcgc cggtgaggcg gacagcctct cccgccgctt tgaggaggtg gaacggctcg
     7981 accgcaccct gcagatggtc ttcgcctggt acggccgcga ggatctcggc gacagcgagc
     8041 ggctgcgcca tctccaccgc accttcgagt tcgatcccct catcgtcaaa gcccgcagcc
     8101 tgcaggacat tcagctcacc gggctctccg agtccggcct gcggttgctc ccgggtgccg
     8161 ccgactcgcg cggccaggtc agcgagcgcc tgctccgctt caccgctgac cgcgacatcg
     8221 aattgcagag cctctacgaa ctcgacggac aggggctgcc catcaccctg aaaaaggcgg
     8281 cgcggccgat gctgctcgat ggactgccct tcttcagctt ttccgaagag ctggccagat
     8341 ttcgcgagga gctgctcttt ctcgccgcca aaaaggtcag cagccagcag gagtacgaca
     8401 gcctcctggc caaggaaaag gctcatctgc gcggcaaaca gttccagctc gacgagcgcg
     8461 agatgaacga actcatcttc aaggctcatg cccccaacga gaccctcagc acggaggaga
     8521 tcgagcagtg gtgccgcctc catttcgagc gcaagccgcg ccgcttgctc gaggagcagc
     8581 gcgatgccct ctggcaggac tataccgcca tcgaggagcg ggtgcagaat atctcccagc
     8641 tcctgcgcgc ctataccctc taccagcgcg acgtcgatta tgtggtcaaa accctcgatg
     8701 aggccgacct gcgcggccgc atctccgggc gtaagggagc gaaggcggtg atgatcgtcg
     8761 accagttcac cgggcgcctc atgcccgggc gccgctacag cgatgggctc cacgaagccc
     8821 ttgaggccaa ggagggggtg gaggtccagg ccgagtccca gaccctggcc accatcacca
     8881 tccagaactt tttccgcctc tacgacaagc tctccggcat gaccggtacg gcggagaccg
     8941 agtcccagga gttcttcagc acctacaaac tcgaggtggt ggtcattccc accaaccgtc
     9001 cggtcgtccg cgatgacgag aacgacgtca tctaccggac gcgcaaggaa aagtactccg
     9061 ccatcgtcga agaggccatc gccatgcacg aggcggggcg ctccgtcctg gtcggcacca
     9121 tctcggtcga ggtctcgcag ctgctcagcg acatgctcac ccagcggggt gtgcccatcg
     9181 ccaactggct caagaagggg gatgtcagcc aggaactgga gtcgggccgt tttcataccg
     9241 tgctcaacgc caagttccat cgccaggagg cggagatcgt cgccaaggcg ggcctgcccg
     9301 gcagcatcac cattgccacc aacatggccg gccgcggcac cgacatcaag ctccatcccg
     9361 aggtggccaa ccgcggcggc ctccacatca tcggctcgga gaaacacgag gcgcggcgca
     9421 tcgaccgcca gctgcgtggc cgctccggac gccagggcga tcccggctcc tcccgcttct
     9481 atctctccct cgaggacgat ctcatgcgcc tcttcggctc cgaccgcatc accaccatga
     9541 tgagccgcct cggccccatg gaggagggcg aacggatcga gcacccgctc atcacccgct
     9601 cgatcgagcg cgcgcagaaa aaagtcgagg agcgcaactt tgagatccgc aagcacctcc
     9661 tcgattacga taacgtgctc aacgatcagc gcaagatcat ctacaagcgc cgccagaacc
     9721 tcctcggctt cgccggggcc gatgatttcg tcgaatccaa ggccaaacgc tatttcaacg
     9781 aggaggacga ccgcggtgaa tgggacctgg ccggcctggt ggacaacctg cgcgattttt
     9841 tcaagaccga tcccccgttc gaggccgaag gccttgaggg actcaagcat gaggagatca
     9901 agtccacggt gcgcgactgg gtggccgagc gtatcgagga ggagagcctg cagcgccagc
     9961 tccaaatccg ccaccgcctc ttcggctatg cgccctttgc gcgcattctg gaggagctgg
    10021 tacacctcaa gatcaggctc cataacgccg gctcccggga tgtctcgcgc tggaatatgg
    10081 gcgggatccg ttacgaactc gagcgcattt tcggcgaggc gccggaatgg ctcgaggaga
    10141 gcagccgggg cgaagatgcg gctgcgctgg agaagcggat cgtcgcctgg gcggcggagc
    10201 tctacaaaac ccgattcgag gcggaccatg ccggctatga tcttgccctt ttcgccaaac
    10261 tcccggccga ggagatcgtc aagatctaca tccacggtct gatgagcctc catcttaatg
    10321 agagcgcgcc ccagatcaac tggacccccg aagagttcct cgccgacctc gagcgcgtct
    10381 ttgaggagcg gccgcagctc ggagtcaacg agatcaggac catccagcgc gggcgcgtga
    10441 gcgccatggt gcggagttgg gccctggaac ggaccggccg tgtagatagc gagaccctcc
    10501 gccaccgcat catcggctat ttctcttccg ccctctttct cgatgtgctg accgactatc
    10561 tcctgacaga attgggggag cccggggaga agacgctgaa cggacgccag caagcgttca
    10621 tctccgggta tttcgggccg gaggccctct tcaccggcag cgccgaaaac cggtccgacc
    10681 tcgcccaggc ggttaaccgc cagctgcaca tccactaccg acagcagctg caggaggaga
    10741 gggagggcta ccttcaggcc atgctcaccc atgcctcgat ggacgagatc ctgaccgccg
    10801 ccatcctcgc catgatcaag tcggtcaccg cgtgggaggg gacgaccgag agcaaacagc
    10861 ggctgctcag cgcgcggctg gagttcattc tccagcagcg cccgcccaaa tcacttcccg
    10921 agcatgggga tgaggccctc gtcgcctcct acgccgagga tgtcgtcgcc tgggccttga
    10981 agtggtacgc cacctatgcg gagcgccagg agcggctgca ccaggagacc ctgagttcgg
    11041 agatcgtgcg cgattcggtg ctgaccatga tccaggatac ggtctatacc atgatcagca
    11101 acctgctttc cggtgacagc gggctggacg ctgcggcgat ccgccgcctc gaatcggact
    11161 gccgtctggt cttccgccag gccccccgcc tctccgacga ttcgatggag ggatacgatc
    11221 ccaacgcgat catggaacag gtgacgcgct gggcggtggc cctctaccag aagcggatcg
    11281 ccgagctggg gcgcgagcgg gccacccgct atgagcgcta tttcgttctg gaaaagatcg
    11341 acgaaaactg gcggcagcac ctcaacgcca ccgacgagct gcgcgaaggc atcggcctgc
    11401 gcggttatgg ccagaaggat cccctgcttg agtacaagcg ggaggccttt gagctcttcg
    11461 tcaagatgaa tgagcgggtc aaccgcgatg tcgtcgccac tctcttcaag gtcttcgacg
    11521 tcgggggcga g
//
LOCUS       DAOPWU010000084        11528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5704_length_11528_cov_5.748279, whole genome shotgun sequence.
ACCESSION   DAOPWU010000084 DAOPWU010000000
VERSION     DAOPWU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5704_length_11528_cov_5.748279"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /gene="bshC"
                     /locus_tag="PKM23_06135"
     CDS             <1..1606
                     /gene="bshC"
                     /locus_tag="PKM23_06135"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNY91087.1"
                     /translation="VIPPEQLPGMNRLAADYCTHAPAAAGFFAGDFSRPEAWLERAVE
                     VGRADHADRGRVIAALRRQLDQLEAGPQSMANLELLASPNALAIVTGQQAGLFGGPLY
                     TLYKALTTCKLARHWSEQLGRPVVPVFYVVSEDHDFVEVQGAGYLEGGHILKKALYSP
                     SSLRERMPVGLVRLEAEIDAVLAELAGSVPEAEFKAEVIADLASCYHPGAGFAGAFSR
                     WYARLLGELGMVFLDPADPELKRLAAPIFRRELEKRITVERIEIANTELLAGGYHTQL
                     PVHPLRPALFLLRDGRHSLERTGNGYRNLHSGEILSIDELMAAPEQLSPKAALRPLFE
                     DYLLPTLAYVGGPGEIAYWAQLKGVYAGFGLPMPMVVPRAGFTLVEGRSRRALERFGI
                     AAAEVVQRPTEILAALRERLVPADLAGELAELKAIIGQRWPRLQGGITALDQTLQVPA
                     EKSLQQLMHTLNQLEQKVVRAAENRESAGQAQIDALTAALLPGGILQERQLNAVPFLC
                     RHGRGLIGELYEAIDAANPGHSVVEL"
     gene            1618..2364
                     /gene="bshB1"
                     /locus_tag="PKM23_06140"
     CDS             1618..2364
                     /gene="bshB1"
                     /locus_tag="PKM23_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNY91088.1"
                     /translation="MEQALDVLAFGAHPDDIELMAGGTIIKLARQGYRVGMISLTRGE
                     MGTRGTPEIRREEFAASAGIMGLAMHSMLDIPDGGVNESEENRLKVIREIRRWRPALL
                     FAPYWETRHPDHAHCSHLVREAAFYSGLKKIESGEAAFRPTRLAYYCEHYHFAPSFVV
                     DISDVFEIRLEAIRAYKSQVFNPDAPADDEERTHISSPEYFRSIYARAEYWGGRIGVR
                     YGEPFLVREVIRTEDPFALLIGRAREEGQG"
     gene            complement(2366..3532)
                     /locus_tag="PKM23_06145"
     CDS             complement(2366..3532)
                     /locus_tag="PKM23_06145"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNY91089.1"
                     /translation="MTTTLPADSAGLVKREYFSFAAPPDELSLDCGRKLGPVTLAYEI
                     YGELNPARDNAILILHALSGDAHAAGWHHPEDHRPGWWDFMIGHGRPFDLDKYCVICT
                     NVIGGCQGSTGPSSIDPETGNPYALNFPMVTIADMVRAQMRLIDHLGINRLLCVTGGS
                     MGGMQALQWSIAAPERVASAIPIATTCHQNAMQIGWDEVGRQAIYADADWCSGDYQRL
                     GKKPARGLAAARMLAHITYLSEESMRRKFGRTLREKENYGYDFGIDFEVESYLRHQGE
                     IFVDRFDANSYLYITKAIDYFDLARDYGSLEEAFKKVQARYLILSFTSDWLYPTSQSL
                     ELLHILQANKKAVTFRELNTWYGHDSFLLPNPEMMRIIADFLAEVHAAGARERP"
     gene            complement(3529..4827)
                     /locus_tag="PKM23_06150"
     CDS             complement(3529..4827)
                     /locus_tag="PKM23_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNY91090.1"
                     /translation="MNTQHRHFETLALHAGGQEDPAASSHAVPVHRTSAYLFRDSAHA
                     ADLFALKESGHIYTRLGNPTQDLLEQRMAALEGGAAALALASGTSAIFYAIINICAAG
                     DEIVAARNLYGGTYTMFDTILPQFGIRCRFVDPTDPAQFEKAINGRTRALYAETIGNP
                     SLDLIDLEEIAAVARRHHLPFIVDSTFTTPWLLRPLEYGADIVCHSLTKWLGGHGTGI
                     GGIVVDKGSFDWSDAKFNLFNQPDPGYHGLRYAHDLGELNPLAFIMRLRLVPLRNLGA
                     CISPDNAWIFLQGIETLALRMQRHCENGLAVAEFLQHHPAVAWVRYPGLPGDPSHVVA
                     KKYLHRGFGGMVVFGIKGDVPAGALFIDSLKLFSHVANVGDARSLAIHPASTTHSQLS
                     EAQRLEGGITPDLIRLSIGLEHIDDLKEDLEQALARAVKW"
     gene            5056..6717
                     /gene="hutU"
                     /locus_tag="PKM23_06155"
     CDS             5056..6717
                     /gene="hutU"
                     /locus_tag="PKM23_06155"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNY91091.1"
                     /translation="MKEAIRAPRGAQLSCKGWIQEAALRMLMNNLDPEVAEKPQELVV
                     YGGTGKAARNWECFHAMVQALRELENDETLLVQSGKPVAVFKTHENAPRVLISNSMLV
                     PHWATWDEFRRLEGLGLTMYGQMTAGSWIYIGTQGILQGTFETFASCAERHFGGTLEG
                     RLLVTAGLGGMGGAQPLAATMNGAACLCIEVDRHRMERRLETRYLDVMCESLDEALEQ
                     VIAARDRREPLSVGLLANAADVLPELVQRRIVPDILTDQTSAHDVLNGYIPHGLAYDE
                     ALALRTADPERYKKMARQSIVRHVEAMLALQRLGAVAFDYGNNIRGEALDNGLKNAFD
                     IPGFVPEYIRPLFCLGKGPFRWAALSGEADDIYATDAALLRTFPENGALRRWISKAQK
                     QVQFQGLPSRICWLGYGERARMGAVFNDLVASGEVKAPIVIGRDHLDAGSVASPNRET
                     ERMRDGSDAIADWPILNALINAVGGASWISVHHGGGVGIGKSIHAGMVIVADGTREAS
                     VRLQRVLTTDPGMGVVRHADAGYEIAVNAASQMGIKIPMLAQGRP"
     gene            6723..7919
                     /locus_tag="PKM23_06160"
     CDS             6723..7919
                     /locus_tag="PKM23_06160"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNY91092.1"
                     /translation="MTDPKVFYRELDALLAMIRIEEAGEQFLPTILQELSAKFHQRLH
                     IADARYYLLLDDLFVLSYPEEGAEEHGYVEELPMESEVVQLASRNRSYIYDSEFLNNQ
                     FYAPAQPTHLSHAAIWVHSPERQWLVVFALNDGWEREEINLFLNSVRTSLNYRLFSDA
                     IGVRLEQAVEIQKSLLPQKSPKLSGYDIAGFSQPAELVGGDLFDYFELKDGTLGVSIG
                     DVSGHGLPAALLMRDVVIGLRMGLALELRTVHTIKTLNRVILRSTYAANYVSLFVAEM
                     EGDGHLFFVNAGHPGPLLVHGKKVKKLIATGTTLGFLPEIDLHRDYAHLESGAVLVLY
                     SDGITERQNEEGEMFGVERLTEWIVDNQHLDASALLHMTFAHVYEFGDSAAWEDDVTL
                     VVIKRR"
     gene            7935..9155
                     /gene="hutI"
                     /locus_tag="PKM23_06165"
     CDS             7935..9155
                     /gene="hutI"
                     /locus_tag="PKM23_06165"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNY91093.1"
                     /translation="MSSLLVVNIAQAVSGSATPARGRKMRELVIHAPAAIYAREGVIQ
                     AVGPRELVEKEVSDDPVVLDAGGGAVIPGFVDSHTHLVFGGQREDEFDLKIAGASYQE
                     IAAAGGGIISTVEATRRAAPSELVETGLGYLRSALAHGTTTMEIKSGYGLDLENELKI
                     LEVIAELGRRQPVELVPTFMGAHAVPPGMSAQEQTQRVIAMLPHVQGKAEYCDVFCEG
                     GYFGLDETRAILAAARRAGLKLRLHADQLSSNGGVQLALNMGARSVDHLEQITPAEII
                     QLADSSCAATLLPGVSLFLHYGYPPARRLIDEGAIVVLASNFNPGSCMCLNLQLIFAL
                     ACTQMRMTTAESLIALTANAAWALDRPHIGRLEPGLQADFLLLDSPGYRMIPYFFGGN
                     HVKVVVKKGEVVVG"
     gene            9148..10071
                     /locus_tag="PKM23_06170"
     CDS             9148..10071
                     /locus_tag="PKM23_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNY91094.1"
                     /translation="MAEPIERYRSFSGYLRERYGGRVWKIPVDAGLSCPNRDGTISRG
                     GCIYCRLESFSRMQSRLDIGLTEQIEAGIRNAQTRQGADKFIIYFQSSSNTRAPVEHL
                     RALYERALSFPGVVGLDIATRPDLLGEEVLALLAGMAERTDLWIELGLQSSHDQTLRA
                     INRGHTYGDYLTAIASLRALPLRICTHLILGLPGEDAAMMLATADAIAASPVHEIKLH
                     PLLILTDTVLAEWHRQGQVRELAPAEYAALAADFIERMPEEMVMQRLTAEAPAEMLIA
                     PRWALAKHKVRRAIEEELVRRDSWQGKCRRL"
     gene            complement(10066..>11528)
                     /locus_tag="PKM23_06175"
     CDS             complement(10066..>11528)
                     /locus_tag="PKM23_06175"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNY91095.1"
                     /translation="LLAPATLLQGREIVIKDRQSGAAIGHLSTIQAGRYEFIPLSAFA
                     EAIKIPGAINSSRKQFILQIPHNPVVVHAINPFVRVGTEWRQMPIAALILDGEYYAPA
                     VYFLDIIRDALPFRLDYLSGEATILITRSRMGVGGIQIDDMQNGTLIRIALSQPINAS
                     NLFLSETNGWLYADLYGGNIGDFRADAARKNTRTIDRISTIQLSRDTARLGFRLLKKV
                     KEREVKVIASPPEIQISLRTHEEVPAALLDELEREREKWKIDLIVIDPGHGGRDPGAL
                     GSSGTREKDFTLKIAREIKRIIERDLKIRVLMTRDSDKFIPLVDRGKIANRAGGKLFI
                     SIHADSNPKKGLRGHTVYFMGPAKTEEARRVAQFENSVIKFEDSNSKYANLSETAFIL
                     AANAQNSYNKESEEFAAILDREMRSRQQADGFGVRQAGFYVLHGTSMPNILLETAFIS
                     NASDEKLLGTRGFHMNVAEAVCESIRQFRDHYENAL"
BASE COUNT         2127 a         3256 c         3877 g         2268 t
ORIGIN      
        1 tgtcatcccg cctgagcagc tgccgggcat gaaccggctt gccgccgatt actgtaccca
       61 tgcccctgct gcggccggct tttttgccgg ggatttcagc cggcccgaag cctggctgga
      121 gcgggcggtg gaggtggggc gcgccgacca cgccgaccgg ggccgggtga tcgccgccct
      181 gcgcaggcag ctcgaccagc tggaggctgg tccgcagagc atggccaacc tggagctcct
      241 cgcctcaccc aatgccctgg ccatcgtcac cggtcagcag gcgggacttt tcggcggccc
      301 cctttacacc ctctacaaag ccctcaccac ctgcaagctg gcgcggcact ggtccgagca
      361 gctgggcagg ccggtagtgc cggtttttta tgtggtctca gaggaccatg atttcgtcga
      421 ggtgcagggt gccggctatc tggagggagg gcatatcctg aaaaaggccc tctattcccc
      481 ctcaagtctg cgtgaacgca tgccggtcgg cctggtgaga ctcgaggcgg agatcgacgc
      541 tgttctggca gaactggccg ggtcggtgcc cgaggcggag ttcaaggcag aggtcatcgc
      601 cgacttggcc tcatgttacc atcccggcgc cggtttcgcc ggggctttct cccgctggta
      661 tgcccgactg ctcggggagc tggggatggt attcctggat cccgccgatc cggagctcaa
      721 gcgcctcgcc gcccctatct tccggcgcga gctggagaag cggatcacgg tcgagcggat
      781 cgaaatcgcc aataccgagc tgctggccgg ggggtatcac actcagcttc ctgtccatcc
      841 cctgcgcccg gccctctttc tgctgcgcga cggccggcac agcctggagc gcaccgggaa
      901 cggataccgc aacctgcaca gcggggagat cctctccatc gatgaactca tggccgcacc
      961 ggagcagctc agcccgaagg cggcgctgcg tcccctcttc gaagattatc ttctgccgac
     1021 tctggcctac gtcggcggac ccggtgagat cgcttactgg gcccaactca agggggttta
     1081 cgccggattc gggctgccca tgcccatggt ggtgccgcgg gccggtttca ccctggtgga
     1141 gggcaggagc aggcgcgccc tggagcgttt cggcatcgcc gcggcggagg tggtgcagcg
     1201 gccgacggag attctggccg ccctgcgcga aagactggtg ccagcggatc tggccgggga
     1261 gcttgcggaa ctcaaggcga taatcgggca gcgctggccc cgcctgcagg gaggcatcac
     1321 cgccctcgat cagaccctgc aggtcccggc cgagaagagt ctgcagcagc tgatgcatac
     1381 cctcaatcag ctcgaacaga aggtagtgcg cgcggcggag aacagggaga gtgcggggca
     1441 agcccaaatc gacgccctga ccgccgccct gctgccgggt ggaatactgc aagagcggca
     1501 gctgaatgcg gtgccttttc tctgccgtca cggccggggc ctgattggtg agctttatga
     1561 ggctatcgat gcagcgaatc ccggacacag tgtagtggaa ctttagccga ggttaaaatg
     1621 gaacaggcgc tggatgtgct cgccttcggg gcccaccctg atgatatcga actgatggcg
     1681 gggggaacga tcatcaaact ggcccggcag ggctaccggg tggggatgat ttccctgacc
     1741 cggggcgaga tgggcacgcg cggcacgccg gagatcaggc gcgaagagtt cgccgcctcg
     1801 gccggaatca tgggtttggc gatgcacagt atgctcgaca tcccggatgg gggtgtcaac
     1861 gagagcgagg aaaaccggct caaggtgatc cgggaaatca gaaggtggag gcccgctctg
     1921 cttttcgcgc cctactggga gacacgtcat ccagaccatg cccactgctc gcacctggtg
     1981 cgcgaggcgg ctttctactc cggcctgaaa aagatcgaga gcggggaggc cgcgttccgt
     2041 cccacgcggt tggcctacta ctgcgagcac tatcacttcg ccccctcctt tgtggtggat
     2101 atttccgatg tcttcgagat ccggctggag gcgatccgcg cctacaaatc acaggtcttc
     2161 aatccggacg cccccgccga tgatgaggag aggacccaca tcagcagccc cgagtatttc
     2221 cgttccattt acgcccgggc ggagtattgg ggcgggcgga tcggcgtgcg ttatggggag
     2281 ccttttctgg tgcgggaagt gatcaggacg gaagatccct tcgccctgct catcggcagg
     2341 gcgcgggagg aggggcaagg gtagatcagg ggcgctcgcg ggcgccggcg gcatggacct
     2401 cggcgagaaa atcggcgatg atccgcatca tctcgggatt gggaagcagg aaagagtcgt
     2461 ggccatacca ggtgtttaat tccctgaagg tcacggcttt tttgtttgcc tggaggatgt
     2521 ggagcagttc gagggattgg gaggtggggt agagccagtc cgaggtgaag gagagaatca
     2581 ggtagcgtgc ctgtaccttt ttgaaggctt cttcgaggga gccgtaatcg cgggcgaggt
     2641 caaaataatc gatggccttg gtgatataga ggtaggagtt ggcgtcgaat cgatcgacaa
     2701 agatctcccc ctggtggcgg aggtagctct cgacctcgaa atcgatgccg aaatcgtagc
     2761 cgtaattttc cttttcgcgc agggtgcggc cgaatttgcg tcgcatcgac tcctcgctca
     2821 ggtaggtgat gtgggcgagc atgcgtgcgg cggccagacc cctggccggt tttttcccca
     2881 gacgctggta gtcgccgctg caccagtcgg catctgcata gatggcctgg cggccgacct
     2941 catcccagcc gatctgcatg gcattctgat ggcaggtggt ggcgatgggg atggcggagg
     3001 cgacccgctc cggcgcggcg atgctccact gcagggcttg catgccgccc atggagccgc
     3061 cggtgacgca gaggaggcgg ttgatgccga ggtggtcgat gagacgcatc tgggcgcgca
     3121 ccatgtcggc tattgtaacc attggaaagt tcaaggcata ggggttaccg gtctccggat
     3181 cgatggagga agggccggtg gagccctggc agccgccgat gacgttggtg caaatgacgc
     3241 agtatttgtc gagatcgaaa gggcggccgt gtccgatcat aaagtcccac caccccggcc
     3301 ggtggtcttc ggggtggtgc cagccggcgg catgggcgtc tccggagagg gcatggagga
     3361 tgaggatggc gttgtcgcgt gcgggattga gctcgccgta gatttcatag gcgagggtga
     3421 ccggccccag tttcctgccg cagtctaggg atagttcatc cggaggcgcg gcaaaggaaa
     3481 agtactctct cttgacgagg ccggctgaat ctgcagggag ggttgtggtc accacttgac
     3541 cgccctggcg agggcctgtt ccaggtcctc cttgaggtca tcgatatgtt cgaggccgat
     3601 ggagaggcgg atgaggtcgg gggtgatacc cccttccagg cgctgggcct cgctgagctg
     3661 ggaatgggtc gtgctcgccg gatggatggc caggcttctg gcgtcgccga cgttggcgac
     3721 gtgggagaag agcttgaggc tgtcgatgaa aagggctccc gccggcacgt cccccttgat
     3781 gccgaagact accatgccgc cgaagcccct gtgcaggtat tttttggcca caacatggct
     3841 gggatcgccg ggcaggccgg ggtagcgaac ccaggcgacc gccgggtggt gctgcaggaa
     3901 ttcagcgacg gccaatccat tttcgcaatg gcgctgcatg cgcagggcga gggtttcgat
     3961 gccctggagg aagatccagg cgttatcagg cgagatgcag gcgccgaggt tgcgcagggg
     4021 caccaggcgc aggcgcatga tgaaggcgag ggggttgagt tcgccgagat cgtgggcgta
     4081 gcgcaggccg tggtaaccgg gatcgggctg gttgaagagg ttgaatttgg catcgctcca
     4141 gtcgaaactc cccttgtcga cgacgatgcc gccgatgccg gtgccgtggc cgcccagcca
     4201 tttggtcagg gaatggcaga cgatgtcggc cccatattcg agggggcgca ggagccacgg
     4261 ggtggtgaag gtggagtcga cgataaaggg cagatgatgg cgtctggcca cagcggcgat
     4321 ttcttcgaga tcgatgaggt ccagggaggg attgccaatg gtctctgcat agagggcgcg
     4381 ggtccggccg ttgatcgcct tttcgaactg tgccggatcg gtgggatcga cgaagcggca
     4441 gcggatgccg aactggggca gaatggtgtc gaacatggtg taggtgccgc cgtagagatt
     4501 gcgggcggcg acgatttcat ccccggccgc gcagatgttg atgatggcgt aaaagatcgc
     4561 cgaggtgccg gaggcgaggg ccagtgccgc ggcgcctcct tcgagagcgg ccatgcgctg
     4621 ctcgagcaga tcctgggtgg ggttgccgag gcgggtatag atatgacctg attccttgag
     4681 ggcgaagaga tcagctgcgt gggccgaatc gcggaagagg taggcgctgg tgcgatggac
     4741 cgggaccgca tgtgaggagg cggccggatc ttcctgcccg ccggcatgga gggccagggt
     4801 ttcgaaatgg cggtgctggg tgttcatact tttatccctg atgagatcga ctgcacttgt
     4861 atgaatataa gaaaaaaaaa gaccaacttg aatcttattc cacagtgttt ttacgggagt
     4921 gagcgttttt accggggcgg aaattatttt gcatccgatg gaaaaattat ctaacttgtt
     4981 gcgcaatggg ggtaaaaact tgctgaacgg ctccgtttga gccgggcccg gtcactaggg
     5041 aaaaaggatg tgagcatgaa agaggccatt cgcgccccgc ggggcgccca gttgagctgc
     5101 aagggctgga ttcaggaagc cgccctgcgc atgctgatga acaatctcga tccggaggtc
     5161 gctgaaaaac cccaggagct ggtcgtctac ggaggaaccg gcaaggccgc acgcaactgg
     5221 gagtgttttc atgccatggt acaggcgctg cgggagcttg aaaatgacga gaccctcctg
     5281 gtgcagtcgg gcaaaccggt cgccgtcttc aaaacccatg agaatgcccc gcgtgtactg
     5341 atctccaact ccatgctggt tccccattgg gcaacctggg acgagtttcg ccgcctcgag
     5401 ggtctgggac tgaccatgta cggccaaatg actgccggca gctggattta tatcggtacc
     5461 cagggtatcc tgcagggaac cttcgagacc tttgccagct gtgcagagcg ccatttcggc
     5521 ggcaccctgg aggggcggct gttggtgacc gccgggctgg ggggcatggg gggcgcccag
     5581 ccgctggcgg cgaccatgaa tggcgcagcc tgtctctgca tcgaggtcga ccgtcatcga
     5641 atggagcgca ggctcgaaac ccgttatctg gatgtcatgt gcgagagcct ggatgaagcc
     5701 ctcgagcagg tgatcgcggc gcgcgaccgc agggagccgc tttcggtcgg tttgctcgcc
     5761 aatgccgcgg atgttctgcc cgagctggta cagcgcagga tcgtgccgga tatcctcacc
     5821 gaccagacct cagcgcacga tgtcctcaac ggctatattc cccacggcct ggcctatgat
     5881 gaggcgttgg cgctgcggac ggcagatccc gaacgctaca aaaagatggc ccgccagtcg
     5941 atagtccgcc atgttgaggc catgctcgcc ctgcagcggc tcggcgcggt ggccttcgat
     6001 tatggcaaca acatccgtgg agaggctctc gacaacggat tgaaaaacgc ctttgacatc
     6061 cccggctttg tgccggaata catccggccg ctcttttgcc ttggcaaggg tcccttccgt
     6121 tgggcagccc tgagcgggga ggccgatgat atctatgcca ccgacgccgc cctgctgcgc
     6181 actttcccgg agaacggagc cctcagacgc tggatcagca aggcacaaaa gcaggtgcag
     6241 tttcagggac tgccctcgcg catctgctgg ctgggctatg gcgaacgcgc tcgaatggga
     6301 gcggtcttca atgacctggt cgcctccggg gaggtcaagg cccctatcgt catcggccgc
     6361 gaccatctcg acgccgggtc ggtggcttcg cccaaccggg agaccgagcg catgcgcgat
     6421 ggcagcgatg ccatcgctga ctggcccata ctcaacgccc tcatcaacgc cgtgggaggg
     6481 gcgagctgga tttcagtcca tcacggcggg ggcgtcggca tcggcaagtc gatccatgcc
     6541 gggatggtga tcgtggctga tggcacgcgc gaggcctccg tacggctgca gcgggtgctc
     6601 accaccgatc ccggtatggg ggtggtgcgc cacgcggatg ccgggtatga aatcgccgtg
     6661 aacgccgcca gccaaatggg gatcaaaatc cccatgctcg cccaggggag gccctgactg
     6721 tcatgaccga tcccaaggtt ttctatcgcg agttggatgc cctcctggcc atgatccgga
     6781 tcgaggaggc aggggagcag tttctgccga ccatcctcca ggagctgtcg gccaaattcc
     6841 atcagcggct tcacatcgcc gatgctcgct actacctctt gctggacgat ctctttgtac
     6901 tcagctaccc ggaagagggg gcggaggaac acggctatgt cgaggaactg cccatggaat
     6961 cagaggttgt gcagttggcc tcccgcaacc gcagctatat ctacgattcc gaattcctga
     7021 acaaccagtt ttatgccccg gcgcagccca ctcatctcag tcatgcggct atctgggtgc
     7081 acagcccgga gcggcagtgg ctggtggtat tcgccctgaa tgacggctgg gagcgggagg
     7141 agattaatct ctttctcaat tccgtgcgca ccagcctcaa ctaccgcctt ttctcggatg
     7201 ccatcggggt gcggctcgag caggcggtgg agatccagaa gagcctgctg ccccaaaaat
     7261 cgcccaaact gagcggctac gatattgccg gcttcagcca gccggcggag ctggttggtg
     7321 gcgacttgtt cgattatttt gagctcaagg acggcaccct gggggtttcg atcggggatg
     7381 tgagcggcca cggcctgccc gcagccctgc tgatgcgcga tgtggtcatc ggtttacgca
     7441 tggggctggc cctggagctg cgcactgttc acaccatcaa gacgctgaac agagtcattc
     7501 tgcgcagcac ctatgcggcc aactatgtct ccctttttgt cgcggagatg gagggcgacg
     7561 ggcatctctt tttcgtcaat gccggccatc cgggtcccct gctggtgcac gggaaaaagg
     7621 tcaaaaagct gattgccacc ggaaccacgc tgggcttttt gccggagatt gacctgcatc
     7681 gcgattatgc acatctcgag agcggggcgg ttctggtcct ttacagtgac ggcatcacag
     7741 agcgtcagaa cgaggagggg gagatgttcg gtgtcgagcg gctgaccgaa tggattgttg
     7801 acaaccagca cctcgatgcc agcgccctgc tccacatgac ctttgcccat gtctatgaat
     7861 tcggggattc agcggcgtgg gaggatgatg tcacgctggt ggtgatcaag cgacgttaaa
     7921 aaggaggtgt gcggatgtcc tccctgctgg ttgtcaatat tgcccaggcg gtcagcggca
     7981 gcgcgactcc ggcgcgcggg cggaaaatgc gcgagctcgt cattcatgcg ccggcggcga
     8041 tctatgcgcg ggagggggtg atccaggctg tcggcccgcg ggagctggtc gagaaggagg
     8101 tcagcgatga tcctgtagtg ctcgacgccg gtggcggagc ggtcatcccc ggatttgtcg
     8161 acagccacac ccatctggtc ttcggggggc agcgcgagga cgaattcgat ctcaagatcg
     8221 ccggagcgag ctatcaggaa atcgccgcag cggggggagg gatcatcagc acggtcgagg
     8281 cgacccgaag ggcggcaccc tccgagttgg tcgagaccgg tctgggctat ctgcgttcag
     8341 cgctggcgca cggcaccacc accatggaga tcaagagcgg ctacgggctc gatctggaga
     8401 acgaactcaa gattctggaa gtgatcgccg agctggggag gaggcagccg gtcgaactgg
     8461 tgccgacctt catgggcgcc cacgctgtgc cgccgggaat gagtgcgcag gagcagacgc
     8521 agagggtgat cgcaatgctg ccgcacgtgc agggcaaggc ggagtactgc gatgtttttt
     8581 gcgagggggg ctattttgga ctcgacgaga ccagggccat actggccgca gccaggaggg
     8641 cgggcctgaa actccggctg catgcggatc agctcagcag caatggcggg gtgcagctgg
     8701 ccctcaacat gggggcgcgt tcggtcgatc atctcgaaca gatcaccccc gcggagatca
     8761 tccagctggc ggattccagc tgtgccgcca ccctgctgcc gggggtgtcg ctttttttgc
     8821 attacggcta tcccccggcg cggcgtttga tcgatgaagg agcgatcgtc gttctggcct
     8881 ccaatttcaa cccgggcagc tgcatgtgtc tcaacctgca gctgatcttt gcgctcgcct
     8941 gcacacagat gcgcatgacg acggccgaat ccctcattgc cctcacagcc aacgcagcct
     9001 gggcgcttga ccgcccgcac atcggacggc tggagcccgg cctgcaggcc gattttctgc
     9061 tcctcgattc tccgggttat cgcatgatcc cctatttttt cggagggaat catgtcaagg
     9121 tggtagtgaa aaagggcgag gttgtggttg gctgagccga tcgaacgcta tcgctctttt
     9181 tcgggctatc tgcgcgagcg ctacggcggg agggtctgga aaattccggt ggatgccggc
     9241 ttgagctgcc ccaatcgcga cggcactata agccgcggtg gctgcatcta ctgccggctg
     9301 gagagttttt cgcgcatgca gtcgaggctg gatatcggtc tgactgagca gatcgaggcc
     9361 gggatacgca atgcccaaac gcgtcagggc gccgacaagt tcatcatcta ttttcagagt
     9421 tccagcaaca cccgcgcccc ggtggagcac ctgcgcgccc tctatgaaag ggcgctttca
     9481 tttcccgggg tggtcgggct cgacatcgcg actcgtcccg atctcctggg ggaggaggta
     9541 ttggccctgc tcgctggtat ggcggagcga accgaccttt ggatcgaact cggcctgcag
     9601 agcagccatg accagaccct gcgggctatc aaccgcggcc atacctacgg tgattatctt
     9661 accgccatcg cgtccctgcg cgccctgccc ctccgcatct gcacccatct catcctcggg
     9721 ctgccggggg aagatgcggc gatgatgctg gcaaccgctg acgccatcgc tgcctctcct
     9781 gtccacgaga tcaaacttca tcccctgctc atactgacgg atactgtatt ggcggagtgg
     9841 caccgtcagg ggcaagtcag ggagctggcg ccagcggaat atgcagcgct ggcagccgat
     9901 ttcatcgaga gaatgcctga ggagatggtg atgcagaggc tgacggcgga ggcgccggcg
     9961 gagatgctga tcgctccgcg ctgggcgctg gcgaagcaca aggtgcgcag ggcgatagag
    10021 gaggagttgg tccggcgcga cagctggcag gggaaatgcc ggaggctata aggcgttttc
    10081 gtagtggtcc ctgaactggc ggatgctttc gcagactgct tcggctacgt tcatgtgaaa
    10141 gccgcgtgtc ccgagcagtt tttcgtccga tgcattggag atgaaggcgg tttcaagcag
    10201 aatattgggc atggaggtgc cgtggaggac ataaaagccg gcctggcgga cgccgaagcc
    10261 gtcagcttgc tggcgtgacc gcatctcccg gtcgaggatg gccgcgaact cctcgctctc
    10321 cttgttgtag ctgttttggg cgttggcggc gaggatgaag gcggtttcgg agagattggc
    10381 gtacttgctg ttggaatcct cgaacttgat caccgagttt tcaaactggg ccacccgcct
    10441 cgcctcttcc gtcttggccg ggcccataaa atagacggtg tgcccgcgca ggcccttttt
    10501 gggattggaa tcggcgtgga tgctgatgaa gagcttgccg cccgcgcggt tggcgatctt
    10561 gccgcggtcg accaggggga tgaatttgtc gctgtcccgg gtcatgagga cccggatttt
    10621 caggtcgcgt tcgatgatcc tcttgatctc ccgggcgatc ttgagggtaa agtccttctc
    10681 cctggttccg ctcgagccca gggcgcccgg atccctgccg ccgtgacccg gatcgatgac
    10741 gatcaggtcg attttccatt tttcccgttc cctctccagc tcatccagca gtgcggccgg
    10801 cacctcctca tgggtgcgca gcgagatctg gatctcgggc ggagaggcga tcaccttaac
    10861 ctccctctcc ttcacctttt tgagcagccg gaagccgagt cgcgccgtat ccctggagag
    10921 ctggatggtg ctgatccggt caatggtgcg ggtatttttc cgggctgcat cggccctgaa
    10981 atccccgata ttgccgccgt agaggtccgc ataaagccat ccgttggttt cgctgaggaa
    11041 gagatttgag gcattgatcg gctggctgag ggcgatgcgg atgagggtgc cgttctgcat
    11101 gtcatcaatc tggatgccgc ccacgcccat acggctgcgc gtgatgagga ttgtcgcctc
    11161 accggagaga tagtccagcc ggaagggaag agcgtcccgg atgatgtcga ggaaatagac
    11221 ggcaggggca taatactcac catcgaggat gagagcagcg atgggcatct gccgccactc
    11281 ggtgccgacg cggacgaagg gattgatcgc gtgaacgacg acgggattgt ggggtatctg
    11341 caggatgaat tgtttgcggc tcgaattgat tgcgccgggt atcttgatcg cttcggcgaa
    11401 ggcgctcagg gggatgaatt cgtagcggcc ggcctgaatg gtggagagat ggccgatcgc
    11461 agcccctgat tgtctgtctt tgataacgat ctcccgaccc tgaagcaggg tcgccggagc
    11521 gaggagaa
//
LOCUS       DAOPWU010000085        11471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5754_length_11471_cov_4.832866, whole genome shotgun sequence.
ACCESSION   DAOPWU010000085 DAOPWU010000000
VERSION     DAOPWU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5754_length_11471_cov_4.832866"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PKM23_06180"
     CDS             <1..977
                     /locus_tag="PKM23_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNY91096.1"
                     /translation="ILISALFLYLAFHKVNMAQMGEAFAAANYWWLLPTVAITFVAHG
                     LRTLRWRYLLEPIQCLSLKPLFSALMIGYFFNDILPAHLGEFVRAYVLGRKQPVSSSA
                     IFGTIVMERIIDVFTLFLLMAIAVMVFPFPDWVRMGGYLTFLVIALLFVALLLLKKYP
                     QPAFRLMERLLGRTMPDLNKRLRKMMESFLVGIVPLRRPSHYLIVALLSAVIWGCYGA
                     AFQLVLYSFDFVATYSLPWTTSLVLLVITTFGVLVPSSPGYVGTYHWLCQQSLALAPF
                     NVPGSQALTFAIVMHAINFLPIIVVGLAMISIEGLSLKSLKSSATRGE"
     gene            complement(990..3392)
                     /locus_tag="PKM23_06185"
     CDS             complement(990..3392)
                     /locus_tag="PKM23_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNY91097.1"
                     /translation="MIIRRVALRNYRRFAEVELECPENIIGIIGRNGAGKTTLVEAIG
                     WALYGNVITRTGKGDIRSQFAGESETCSVELEFEYGGEEYRILRQLRGKNAVAEAAIY
                     RAGEAAPDAVQERGVNEYVEQLLRLDYRSFYASVFARQRELAALSAMPPEERRKSIAR
                     LINIDRIDKARERVLADRNGKNRYIEGMRSALIDPDLLEQRIADAMTESLQLDEAMAR
                     EAEAVARHELGLGEARAVMERLAGLRSAWQEHQNRILGWQASRKEQLAALQRSREELK
                     LIAAAEEELETMSGTLQGLTAARSEKERLDGEELRQVQRAGLLEQISQNGEIVSAGRG
                     RIGAARNRRAGLQALAEERAALLRREEELEKSLSEARELQQHWKSEGAAAGKAGTETR
                     TRKEQIEKLGPDGECPTCTQKLSGHYPLVLVELEEKLAALRLAWQTAVKNLETAEKSV
                     AEQELALRTLRREKESLLRRITADTEAAADLERMEEELAAAEKRLAELEQTLAGLGEV
                     IYDRETHLQARARLAELEAMQRRADQLTERASRRGQVAAEIGRTERMTGELTRMEEQE
                     LALQAALGYEESDFQAADAAVKAAMSTMIEAQKSHASAREAAARSRAVEENLQAQKEE
                     QEKKRQEIAKTADEIRYLDALDLYLKKFRVDLAGRIRPLLARRASELLGLTTRGRYTR
                     LELDEEYQISLYDGPRSFPLGRFSGGEQDLANLCLRVAISQILAERSGGAPINFIVLD
                     EIFGSQDEERKSLILDALVQLSSQFRQIFMITHVESIRDNMQVIVEVGLVDEQRSEVR
                     ML"
     gene            complement(3400..4527)
                     /locus_tag="PKM23_06190"
     CDS             complement(3400..4527)
                     /locus_tag="PKM23_06190"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HNY91098.1"
                     /translation="MGIERICHLSDTHLGYSEYGKISPRTGLNQREQDFYDTWDECIG
                     RIIALRPDLVVHAGDLFHTPRPSNRAIRVALAGIQQISAAGIPMVLISGNHETPRIRS
                     TGSIFESLALFSGVHCVYEGEYRRLAFDGLDIHAIPHCSLSEELEMACRSIEIRAGAG
                     CNLLVAHGTWTQTESFSMGEFNEQRLPDFTALYPDAFDYIALGHYHRRVDVAPRVSYC
                     GSTERTSLNQAGYPCGFLELDLAGGERTYHELPARPMVRIQPISCRGLRLQEIYDRVA
                     GEAQSVPEGAIVQLNLAEVEESQFLGMDMRLLEELFPAAFYLEKSISYEAADAVTHSP
                     SLHFDSLGVEFGRFIAALPEMELDRETLFRIGSRYLEEDEE"
     gene            4779..6191
                     /locus_tag="PKM23_06195"
     CDS             4779..6191
                     /locus_tag="PKM23_06195"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY91099.1"
                     /translation="MKKSLLIIAHDRQLLTLFNQAAPNLGFELHSTRKYEEALRMLGE
                     VSSPDAVLLDADTLRAELPKSISEIKSLSDNTLIVLLGALSAEELVDCVKMGADGFFE
                     KPVHKAEQVLRHIEHLLLQLSHRSSSPQHASDQGMTEAPILVGQSEPVRELKNMVQKV
                     APLDATVLITGETGTGKEVIARMIHVMGPNRHNNFLAVHCGGIPDTLLESTLFGHEKG
                     SFTGAYRTHKGYFEIADQGTIFLDEIGDTTPSFQVKLLRVLQDKHFRRIGGTELLSTS
                     ARIIAATNRDLRKMVQEGHFREDLFFRLNVITLRMPPLRERPDDIALLIRHFVSVYSK
                     KHNHLGVYLKPETIEILTHQPWRGNVRELENVIERLVALSDSDWIGPDELPDDYLGKD
                     EGECSETGGFHSYADAKNLFEKEYITQLLLQARGNISRAAKMAQMPRQNLHLKIKKHK
                     IKSDSIRSAQSAAEAVGSEA"
     gene            complement(6201..7097)
                     /locus_tag="PKM23_06200"
     CDS             complement(6201..7097)
                     /locus_tag="PKM23_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91100.1"
                     /translation="MQVYFFTQHGSWQRSGASAEELHRLLERIAAETDTADLVVAVHL
                     VRRSRWSGGTAFVRQWHHPAAFFATRGKWRLGGKRVIPPELPARFKLIRLLLQDDGAP
                     YPMTQMDRYGWQHRFERFSDHLAFFFAHELHHFRRYHLGLHPREGEHSANGWALAHCR
                     ELGVGVASQRPSKKKARKRAHRGPAFWQLLNPIDFLPAAQAAWLWRRGGALLGMAVRL
                     GCGDRDKYVAAKMEHIQRLGAMTPGVQLWITFDPGQRYRNAPVRLVRQMRRPSLRAVI
                     ETGDGKTWRWPLAWLSPDPPVG"
     gene            complement(7235..7903)
                     /locus_tag="PKM23_06205"
     CDS             complement(7235..7903)
                     /locus_tag="PKM23_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91101.1"
                     /translation="MKLHLSLTALALMVMGCSRPQVTPPVRKAVDLGLPGTAALRLTP
                     QQEPRPADLLLLTPPEMPEKAVLKFAEKSSQQGFRILWVRSALVADAEAAAVLLQRAA
                     FQMESGGKKGVMLFGAWPGAAALLTDTTFRALLWIAAPQETAVPLAAADSSASRPRLA
                     LIVPDPAPVTLTPRLKGWLRTSEDLVWLTTAQPAAALLESDLEPIIRRKAQLFFDRHL
                     KGKR"
     gene            8055..10040
                     /locus_tag="PKM23_06210"
     CDS             8055..10040
                     /locus_tag="PKM23_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNY91102.1"
                     /translation="MEPEKTSLPENAYRPLKEGEKYEPVVPVAKPLREVTPYSLIWGL
                     FYAALFSMAAAYLGLKIGQVFEAAIPIAILAVGTSVFLRRKNAMSENVIIQSIGAASG
                     VVVAGAIFTIPAIYILNLDAKFYQIFLASLFGGFLGILFLIPFRKYFVQEMHGQFPFP
                     EATATTEVLVAGEAGGDQAKVLLKAMAIGGLYDFIIGTFHWWSEVFESRAIPFLTGIA
                     DKWKLVFRLNVGAAVTGLGYIVGLKYSAIIAAGSFVSWFLLVPLVHYFGVGLTATLGS
                     TATSLIKDMSAEQIFFTYVRHIGIGGIAMAGIIGILKSSRIIGGAMKLAWAEITGKHS
                     GAEKGLNRMEKDLPMGIIVGAIALTALLILLFFQFGVVHNWLQAIVGLIIVLVISFLF
                     TTVAARAIAIVGTNPVSGMTLMTLILSSFILVKVGMTGTSGMISALIIGGVVCTALSM
                     AGGFVTDLKIGYWLGTTPVNQEKYKFLGTLVAAGSVGFVIMILNETYGFVGPEALVAP
                     QANAMAAVIKPLMSNMPAPWLLYAVGAIFALVLQMIGIPPLAFALGMYIPLELNTPLL
                     VGGLIAHLVSTASKDESINSKRREKGTLIASGFIAGGAIMGVVSAFLKYLGFDKVSFI
                     VPFITLEWAESHSGQLLALALFALLCLYFYRDSKKAA"
     gene            complement(10155..10577)
                     /locus_tag="PKM23_06215"
     CDS             complement(10155..10577)
                     /locus_tag="PKM23_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNY91103.1"
                     /translation="MKRHLFLWILAAALIAAAPVLAAEGNPLDQKPVDKEAAAKLLPY
                     ITHQKIQVRAAIAELLGENCCKGAADALVNLMKTDEVPGVRMVAAQALAKAKVYSAIA
                     EMRDQARKDDNQTVRHVLTALAAELEIQNGTEKSLAAK"
     gene            complement(10832..>11471)
                     /locus_tag="PKM23_06220"
     CDS             complement(10832..>11471)
                     /locus_tag="PKM23_06220"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNY91104.1"
                     /translation="ADLSSLFLDEIGDLSPAVQAKLLRFLQDGVFQRVGGSQNLRSRI
                     RLISATNRDLLYAIADGAFRQDLYYRINVVQIELPPLREHPEDIPLLAEYYARQFAGK
                     YRLAHQRLHPDIDDWLRSYSFPGNVRELINIVERMVILGNGPAAIATLPCIGAVASPD
                     SPRHLPQSLEILEREHIQAILKETGCNKSAAARILGIARKTLREKMVKYHLQ"
BASE COUNT         2045 a         3678 c         3361 g         2387 t
ORIGIN      
        1 gcatacttat cagcgccctc tttctttatc tggcgtttca caaggtgaac atggcccaga
       61 tgggcgaggc ctttgccgcg gccaattact ggtggctcct ccccaccgtt gcgatcacct
      121 ttgtagcgca cgggctgcgc accctgcgct ggcgctacct gctcgagccc atccagtgtc
      181 tctccctcaa acccctcttt tcggccctga tgatcggcta ttttttcaac gatattctgc
      241 ccgcccacct cggtgaattc gtccgcgcct atgtcctcgg cagaaaacaa ccggtttcga
      301 gcagtgcgat ctttggcacc atcgtcatgg agcgcatcat cgatgtcttc accctctttc
      361 tgctcatggc catcgcggtg atggtctttc ccttcccgga ctgggtgcgt atgggcggct
      421 atctgacctt tctggtcatc gccctgctct tcgtcgccct gctgctgctt aaaaagtatc
      481 cgcagccagc tttcagactg atggaacggc tgctcggccg caccatgccc gatctcaata
      541 agcggctcag gaagatgatg gaatcctttc tcgtcggcat cgtgcccctg cgccggccct
      601 cccattatct catcgtcgcc ctgctctcgg ccgtgatctg gggctgttac ggggccgcct
      661 tccagctcgt cctctactcc ttcgatttcg tcgccaccta ttccctgccc tggaccacct
      721 cgctcgtgct gctggtgatc accaccttcg gtgtgctggt gccctcatcc cccggctacg
      781 tcggcaccta tcactggctc tgccagcagt ccctggcgct cgcccccttc aacgttcccg
      841 gcagccaggc cctgaccttc gccatcgtca tgcacgccat caattttctg cccatcattg
      901 tggttggcct cgccatgatc tcgatcgagg gtctgagcct caaaagcctg aaatccagcg
      961 ccacccgcgg cgaataggca gcgtccgcct cagagcatgc ggacctcgct gcgctgttcg
     1021 tcgacgagcc cgacctccac aatgacctgc atgttgtccc tgatcgactc gacgtgggtg
     1081 atcatgaaga tctgcctgaa ctgtgaggag agctgaacga gggcatcgag gatgaggctt
     1141 ttgcgttcct cgtcctgcga gccgaagatt tcatcgagga cgatgaaatt gatcggggcg
     1201 ccgccgctgc gttcggcgag gatctggctg atggcgacgc gcaggcagag attggccaga
     1261 tcctgctcgc cgcctgaaaa gcggcccagg gggaagctgc gggggccgtc gtagagggag
     1321 atctggtatt cctcgtccag ctccaggcgc gtatagcgac ccctggtggt caggcccagc
     1381 agctccgagg cgcggcgggc gaggagcggg cggatgcgcc cggccagatc gacgcgaaat
     1441 ttcttcagat agaggtcgag ggcgtccagg tagcggatct cgtccgctgt ctttgcgatc
     1501 tcctgtcgtt tcttttcctg ctcctctttc tgggcctgca ggttttcctc gacagcgcgg
     1561 ctgcgcgccg ccgcctcccg agcgctggca tggctctttt gggcctcgat catggtgctc
     1621 atggcggcct tgaccgctgc atcggccgcc tggaagtcgc tctcttcata gccgagagcg
     1681 gcctggaggg ccagctcctg ctcctccatc cgggtgagct cgccggtcat cctttcggtg
     1741 cgcccgatct ccgccgccac ctgtcccctg cggctggccc gttccgtgag ttgatcggcc
     1801 cggcgctgca tggcctccag ttccgccagc ctcgcccgtg cctgcaggtg cgtctcgcgg
     1861 tcatagatta cctcgcccag tcctgcgagg gtttgctcca gttctgccag ccgtttctcc
     1921 gccgctgcca gctcctcctc catgcgctcg aggtcagcag ccgcctcggt gtcggcggtg
     1981 atccgacgca gcaggctctc cttctcgcgg cgcagagtcc gcaaagccag ctcctgctcc
     2041 gccacgctct tttccgccgt ctccagattc tttaccgccg tctgccaggc gaggcgcagt
     2101 gcggcgagtt tttcctccag ctctaccagg accagcgggt agtggccgga cagtttctgg
     2161 gtgcaggtcg ggcactctcc gtcggggccc agtttctcga tctgttcctt tctggtgcgg
     2221 gtctccgtgc ccgccttgcc ggccgccgct ccttcactct tccagtgctg ctggagctcg
     2281 cgcgcctcgc tcaggctctt ttccagctcc tcctcccgcc gcagcagggc ggcgcgttcc
     2341 tcagcgaggg cctgcagccc cgcccgcctg ttccgcgcgg cgccaatgcg cccgcgaccc
     2401 gcagagacga tctctccatt ctggctgatc tgctcgagca ggcctgcgcg ctgcacctgg
     2461 cgcagctctt ctccatccag ccgctccttc tcgctcctcg ctgctgtgag cccctgcagg
     2521 gttccactca tggtctccag ctcctcctcc gccgcagcga tcaacttgag ctcctcccgg
     2581 ctgcgctgca aagccgccag ctgctccttg cggctcgcct gccagccgag aatccggttc
     2641 tggtgttcct gccaggcgct gcgcaacccg gcaagacgct ccatcactgc gcgcgcctcc
     2701 ccgagcccca gctcatgccg ggccaccgcc tcggcctccc gcgccatcgc ctcgtccaac
     2761 tgcagactct cagtcatggc gtctgcgatg cgctgctcga gcagatcggg atcgatgagg
     2821 gcgctgcgca tcccctcgat gtacctgttc ttgccattgc ggtcagccag cacccgctcg
     2881 cgcgccttgt cgatgcggtc gatattgatg aggcgggcga tgctcttgcg ccgctcctcc
     2941 ggcggcatgg ccgagagggc ggccagctcg cgctgccggg cgaaaaccga ggcgtaaaaa
     3001 gaacgatagt cgaggcggag cagctgttcg acgtactcgt tgaccccgcg ctcctggacc
     3061 gcatcgggcg ctgcctcacc tgcccggtag attgccgcct ccgcgacggc gttcttgccc
     3121 ctaagctggc gaaggatgcg gtactcctcc cctccatact cgaattcaag ctcaaccgaa
     3181 caggtttcgc tctccccggc gaactgggaa cggatatccc ctttgccggt gcgggtgatg
     3241 acattgccgt agagggccca gccgatcgcc tcgaccaggg tggtcttgcc cgccccgttg
     3301 cggccgatga tgccaataat attctccgga cactccagct ccacctccgc gaagcggcgg
     3361 tagttgcgca gcgccactct tctgatgatc atctccgcct tactcctcgt cctcctccag
     3421 ataacggctg ccgatccgga agagggtctc ccggtcgagt tccatctccg gcagcgcggc
     3481 gataaaacgg ccgaattcca cccccaggga gtcaaaatga agggaggggg agtgggtgac
     3541 ggcatccgcg gcctcgtagc tgatgctttt ttcgagataa aaggcggcgg gaaacagctc
     3601 ctcaagcagg cgcatgtcca ttcccagaaa ctggctctcc tcgacctcag ccaggttcag
     3661 ctgaacgatg gccccctccg gcacggattg cgcctccccg gccacgcgat catagatctc
     3721 ctgcagccgg aggccgcggc agctgatggg ctggatgcgc accatcggcc gcgccgggag
     3781 ctcgtgatag gtgcgctctc cgcccgccag atcgagttca agaaatccgc agggatagcc
     3841 ggcctggtta agactggtgc gttcggtcga tccgcagtag ctcacccgcg gggcgacatc
     3901 gacgcggcgg tggtaatgac ccagtgctat ataatcaaag gcatcggggt aaagggcggt
     3961 aaaatcggga aggcgctgtt cattgaactc gcccatgctg aagctctcgg tttgcgtcca
     4021 ggtgccatgg gcgaccagaa ggttgcatcc ggccccggcc ctgatctcga tgctccggca
     4081 agccatctcc agctcttcgc tcagcgaaca atggggtatg gcatgaatat caaggccgtc
     4141 gaaggcgagc cgccggtact ccccctcgta gacgcagtgg accccggaaa aaagggcgag
     4201 tgactcaaag atggagccgg tgctgcgaat gcgcggggtc tcgtggttgc cggagatcag
     4261 caccatgggg atgccggcgg cgctgatctg ctggatcccg gccagggcca cccggatcgc
     4321 ccggttgctc ggcctcggtg tatgaaagag atcgccggcg tgcacgacca gatcgggccg
     4381 gagtgcgatg atgcggccga tgcactcgtc ccaggtgtcg taaaagtcct gctcgcgctg
     4441 attgagccct gtgcgcggtg agatcttgcc gtattcggaa tagcccagat gggtatcgga
     4501 aagatggcag attcgttcaa tccccaaaac agctcctccg ctcgtttgct taatccgtgc
     4561 cacatcctgt ggcatggctt atacatcttt agcccccgcc gggggagtgt gcctccacgg
     4621 cggcggggga ggccaaaatt attattgtta tttatatact tacataatta caatttcgtc
     4681 gtgattcgtc catttcaggc atgttctttg cttggagggg tttgaaaacc accatcatgt
     4741 gaaccgggat cgttcaggag aataaaggac caggttatat gaaaaaatcc ttgttgatta
     4801 ttgcacacga caggcagctt ttgacacttt tcaatcaggc agcgccgaac cttggctttg
     4861 agctccattc aacacggaaa tacgaagagg ccctgcgcat gctgggtgag gtttcctccc
     4921 cggatgccgt gctcctggat gccgacactc tgcgcgcaga gctgccgaaa tcgatcagcg
     4981 agatcaagtc cctctcggac aataccctga tcgtgctgtt gggcgccctc tctgccgaag
     5041 aactggtcga ctgcgtcaag atgggcgccg acggcttttt tgaaaaaccg gtccacaagg
     5101 ctgaacaggt gctgcgtcat atcgagcacc tcctgctcca gctttcgcac cgctcctcct
     5161 cccctcagca cgcttccgac cagggcatga ccgaagcccc catcctggtc ggccagagcg
     5221 agccggtcag ggagctgaag aacatggtgc aaaaggtcgc accgctcgat gccactgtgc
     5281 tcatcaccgg cgaaaccggc acgggcaagg aggtgatcgc gcgcatgatc catgtcatgg
     5341 ggcccaaccg gcacaacaac tttctcgccg tccactgcgg cggcatcccg gacacccttc
     5401 tggagagcac cctgttcggc catgaaaagg gttcttttac cggcgcctac cgcacccaca
     5461 agggctattt cgagatcgcc gaccagggca ccatttttct cgatgagatc ggcgatacaa
     5521 ccccctcttt tcaggtcaag ctgctgcgcg tactgcagga caagcatttc cgcagaatcg
     5581 gcggcaccga gctgctcagc accagtgcca ggatcatcgc ggccaccaat cgcgatctgc
     5641 gcaagatggt ccaggagggt catttccgcg aggatctctt cttccggctg aatgtcatta
     5701 ccctgcgcat gccccccctg cgcgaacgtc ccgatgacat cgccctcctc atacgccact
     5761 tcgtctcggt ctattccaaa aaacacaacc atctcggggt ctatctcaag cccgaaacca
     5821 tcgaaatcct cacccatcag ccctggcggg gcaacgtgcg cgagctcgag aacgtcatcg
     5881 agaggctggt tgccctctcg gactcggact ggatcggccc cgacgagctg cctgatgact
     5941 atctcggcaa ggatgagggc gaatgttcgg agacaggcgg cttccattcc tatgcggatg
     6001 caaaaaacct gtttgaaaaa gagtatatca cccaactttt actgcaggcg cggggcaata
     6061 tctcccgggc agccaaaatg gcccagatgc cgcggcaaaa ccttcatctc aagattaaaa
     6121 aacacaagat caaatcggat tcgatccgct cagcgcaatc cgcagcggag gcggtgggca
     6181 gcgaagcctg atcctcggcc tcatcccaca gggggatcgg gcgagagcca ggccaggggc
     6241 cagcgccagg ttttgccatc cccggtttcg atcaccgcgc gcagggaggg ccggcgcatc
     6301 tgcctaacca gccgcaccgg ggcgttgcgg tagcgctgtc cgggatcaaa tgtgatccag
     6361 agctgcaccc ccggcgtcat cgcgcccagc cgctggatgt gctccatctt ggcggcgaca
     6421 tatttatccc ggtcgccgca gcccaggcgc accgccatgc cgaggagcgc gccgcccctg
     6481 cgccagagcc aggccgcctg agcggcgggc aaaaaatcga ttggattcaa cagctgccag
     6541 aaggccggcc cgcgatgggc ccgctttcgg gctttttttt tgctcggccg ctggctcgcc
     6601 acgccgacgc cgagctcgcg gcagtgcgcc aaagcccagc cattggcgct gtgctccccc
     6661 tcgcgggggt gaaggccgag atgatagcgg cggaaatggt gcagctcgtg cgcaaaaaag
     6721 aaggcgaggt ggtcgctgaa tcgttcaaag cggtgctgcc agccgtagcg gtccatctga
     6781 gtcatggggt agggagcgcc gtcatcctga agaagcaggc ggatgagctt gaagcgtgcg
     6841 ggcagctccg gtgggatcac ccgcttaccg cccaggcgcc atttgccgcg ggtggcaaaa
     6901 aaagcggcgg gatggtgcca ctggcgcaca aaggcagtgc cgccgctcca acgggaacgg
     6961 cggaccaggt ggacagcgac gaccagatca gcggtatcgg tctccgcggc aatgcgctcg
     7021 agcagccggt gcagttcctc tgccgaggcg ccgctgcgct gccagctccc gtgctgggta
     7081 aagaaataga cctgcatgct tccctgcctc cccgcctgaa cctataaaga ggagcccgaa
     7141 cgccgctgaa gcagcgtcac aaaggtcagc gcgttcggct gagggtcgca aagcctctcc
     7201 gtaaaggttc ccgcgcctcc cggcagcccc gtcatcatcg tttccccttg agatggcggt
     7261 cgaagaagag ctgggccttg cggcggatga taggctcgag gtcggattcc agcagggcgg
     7321 cggccggctg tgcggtcgtc agccagacca gatcctcgga ggttcggagc cagcctttta
     7381 gtcggggggt gagggtgacc ggcgcgggat ccggaacgat gagggccagc cgggggcggc
     7441 tggcggagct gtctgcagcc gccaggggga ctgccgtctc ctgcggagcg gcaatccaga
     7501 gcagagcccg gaatgtcgta tccgtcagca gggcggctgc tccgggccag gccccgaaga
     7561 gcatcacgcc ctttttaccc ccactctcca tctggaatgc agcgcgttgg agcagcaccg
     7621 cggcggcctc cgcatccgcc accagggccg accggaccca aaggatgcga aagccctgtt
     7681 ggctgctctt ttccgcgaac ttgagcacgg ccttttccgg catctccggc ggcgtgagga
     7741 gcagcaggtc ggccggccgg ggctcctgct gcggggtcag gcggagcgcg gccgtcccgg
     7801 gcaggcccag atcgacggct ttgcgcaccg ggggagtgac ctgcgggcgg ctgcagccca
     7861 ttaccatcag cgccagcgcg gtcaggctca gatgtagctt catcggtttc tcctgtggtc
     7921 gttgccgcgt aatatacggc ttcagggggc aaaattaaag cactattttg cgggacacat
     7981 tctccttgca ttaaaggcgg ttgatcgcta tatttttgcc agttatcagc tgtatcaccc
     8041 aaagtggagg agcaatggaa cccgaaaaaa cgtccctgcc cgaaaatgcc tatcgccccc
     8101 tcaaagaggg agaaaagtat gagccagtgg tgccggtcgc caagcccctg cgtgaagtca
     8161 ctccttattc gctgatctgg ggcctttttt atgcagcgct cttttccatg gcggctgcct
     8221 atctcgggct caagattggc caggtcttcg aggcggcgat tcctatcgcg atcctcgccg
     8281 tcggcacctc ggtctttctg cgccgcaaaa acgccatgtc cgagaatgtg atcatccagt
     8341 ccatcggtgc ggcttcgggg gtggtggtcg cgggggctat tttcaccatc ccggccatct
     8401 atatcctcaa tctcgatgcc aaattctatc agatcttcct cgcctccctc ttcggcggtt
     8461 ttctcggcat ccttttcctg attcccttcc gcaagtattt cgtgcaggag atgcacggcc
     8521 agttcccctt ccccgaggcg acggcgacca ccgaggtgct ggtggccggc gaggctggcg
     8581 gcgatcaggc caaggtcctg ctcaaggcca tggccatcgg cggtctctac gattttatca
     8641 tcggcacctt ccattggtgg agcgaggtat ttgaaagccg ggcgatccct tttctcaccg
     8701 gcatagccga taaatggaaa ctggtcttcc gactcaatgt cggcgccgcg gtcaccggtc
     8761 tgggttatat tgtcggcctg aaatattcgg ctataatcgc cgccggcagc ttcgtctcct
     8821 ggtttctgct cgtgcccctg gtccactatt tcggcgtggg tctgaccgcc accctgggca
     8881 gcaccgccac cagtctgatc aaggatatgt cggccgagca gatctttttc acctatgtac
     8941 gtcacatcgg catcggcggc atcgccatgg ctgggatcat cggcatattg aaatcctcac
     9001 gcatcatcgg cggagcgatg aagctggcct gggcggagat caccggcaag cacagcggcg
     9061 ccgaaaaggg cctcaaccgg atggaaaagg acctgccgat ggggattatc gtcggggcca
     9121 tcgccctgac tgcactgctc atcctccttt tcttccagtt cggcgtcgtc cataattggc
     9181 tgcaagccat cgtcggcctg atcattgtgc tggtgatttc cttcctcttc accaccgtgg
     9241 ctgcgcgcgc cattgccatc gtcggtacca atcccgtctc cggcatgacc ctgatgaccc
     9301 tgatcctctc ctcatttatc ctggtcaagg tcggcatgac cggcacttca gggatgatct
     9361 cggccctgat catcggcggg gtggtctgca ctgccctctc gatggccggc ggtttcgtca
     9421 ccgatctcaa aatcggctac tggctcggca ctactccggt caaccaggaa aaatacaaat
     9481 ttctcggtac cctggtcgcc gccggatcgg tcggatttgt catcatgatc ctcaacgaaa
     9541 cctacggctt tgtcgggccc gaggccctgg tcgccccgca ggccaacgcc atggccgcag
     9601 tgatcaagcc cctgatgtcc aacatgcctg ccccctggct gctttacgcc gtcggcgcca
     9661 tcttcgccct ggtactgcag atgattggca taccgcccct ggccttcgcc ctgggcatgt
     9721 atatccccct ggagctgaac accccgctgc tggtcggcgg tctcatcgct catctggtct
     9781 ccaccgcctc gaaggatgag agcatcaaca gcaaacgccg ggagaagggc accctgatcg
     9841 cctcaggctt tatcgccggc ggggctatca tgggcgtcgt ctcggccttt ttgaaatatc
     9901 tgggattcga caaggtctcc tttatcgtcc ccttcatcac cctcgaatgg gcggaaagcc
     9961 acagcggcca gctcctggcc ctcgccctct ttgcgctgct ctgtctctat ttctaccgcg
    10021 attcaaaaaa agcggcctag caccccgaac gtaaaaaagg tgcagggatg cacaataaaa
    10081 aaggctgcct catgcgaggc agcctttttt gctgctcatc agcggtcgat gcgagatcca
    10141 tcgattggtc tcccttattt ggcggccagg ctcttttcag tcccgttctg gatctccagt
    10201 tcggcggcga gtgcggtcaa aacatggcgg acggtctggt tgtcatcctt gcgggcctga
    10261 tcccgcattt cggcgattgc gctgtaaacc ttggccttgg ccagggcctg ggcggcaacc
    10321 atgcgcactc ccggcacctc atcggtcttc atcagattga cgagggcatc cgcagccccc
    10381 ttgcagcagt tctcgcccag caactcggcg atggcggcgc ggacctggat tttctgatgt
    10441 gtgatatagg gcagaagctt cgcagcagcc tccttgtcga ccggtttttg atccagcggg
    10501 ttaccctccg cagccaatac aggagctgcc gcgatcaggg ctgccgccag aatccacaaa
    10561 aacagatggc gtttcatggt gttcctccct tcatgacagt aaagtcctgc cgcacaactg
    10621 aaatctcctg cggcctctgc tgcaacacca tgcaaggagg gtgccagaaa acgttatgac
    10681 gacaaattat gcaagtaatt gtatttattg agctgttttg ccgcggtaag gaaatagttc
    10741 caatccggac tggcaccttt cccccagtct ggaacctttg gtaccagcca tggtggaccg
    10801 gccgccggac tcagccgggc gggagcaggg atcactggag atggtatttg accatttttt
    10861 cacggagggt tttgcgggcg ataccgagga tgcgggcggc cgcgctcttg ttgcagccgg
    10921 tctctttaag gatcgcctga atatgctccc gctccagaat ttccagactc tgcggcaagt
    10981 gacgaggaga atcgggggag gcgacggccc cgatacatgg cagggtggcg attgcggcag
    11041 ggccgttgcc gagaatgacc atgcgttcga cgatattgat gagctcgcgg acgttgccgg
    11101 gaaaactgta gctgcgcagc cagtcgtcga tatcaggatg cagccgctgg tgcgccaggc
    11161 ggtatttgcc ggcgaattgg cgggcgtagt attccgccag cagggggatg tcttcagggt
    11221 gctcgcgcag ggggggaagt tcaatctgca cgacgttgat gcggtaatag aggtcctggc
    11281 ggaaggcacc gtcggcaatg gcatagagga ggtcccggtt ggtggcggag atgaggcgga
    11341 tgcggctgcg cagattctgg ctcccgccca cccgctgaaa gaccccatcc tgcagaaacc
    11401 ggagcagttt cgcctgcaca gccggactca aatcgccgat ctcatcgagg aagaggctgg
    11461 acaggtcggc g
//
LOCUS       DAOPWU010000086        11267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5857_length_11267_cov_4.882626, whole genome shotgun sequence.
ACCESSION   DAOPWU010000086 DAOPWU010000000
VERSION     DAOPWU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11267
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_11267_cov_4.882626"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1327)
                     /gene="nuoF"
                     /locus_tag="PKM23_06225"
     CDS             complement(56..1327)
                     /gene="nuoF"
                     /locus_tag="PKM23_06225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017263697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNY91105.1"
                     /translation="MSEKYFLHNIGKPESKSLAFYLANQGYSAWEKVVRQWQPAEVIE
                     EVKKSGLRGRGGAGFPTGMKWSFVPRDSEKPKYLVCNADESEPGTFKDRYLVEHEPHM
                     LLEGIAIASYAINAHSAYIYIRGEFAHGAQVLEEAIAEAEAKNLLGEKILGSSYDLQV
                     TVHRGAGAYICGEETGLLESLEGKRGWPRLKPPFPAVVGAFKSPTVVNNVETLANLPL
                     IFTRGAEWFAAIGPNPKNTGPKLYCVSGHVNRPGLYEFPMGKNLKELIYEECGGIRGG
                     KKLKAVIPGGSSVPVLRADQIDVSMDYEALAEAGSMLGSAGVIVMDEDACMVNALYNL
                     ERFYAHESCGQCTPCREGTTWMVKILKRLEMGGGRPEDLPLLEDIARHINFRTICPLG
                     DAAAMPAASFLKQFRDEFAAHAELGRCPQRR"
     gene            complement(1347..1820)
                     /locus_tag="PKM23_06230"
     CDS             complement(1347..1820)
                     /locus_tag="PKM23_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNY91106.1"
                     /translation="MSIAFSATAQKRVEKLLGHYPDKEAALLQVLHIARAEFKTITPE
                     VMEYIAGLLGLSPLQVMDVVSFYTLYPRQEEGRYVIQVCATLSCALLGAESLVDHLEE
                     KLGIKVGGTTPDKKFTLKKVECLGSCGTAPVMQINDDYYENLTVEKIDDILAKLP"
     gene            complement(1915..3159)
                     /gene="nuoD"
                     /locus_tag="PKM23_06235"
     CDS             complement(1915..3159)
                     /gene="nuoD"
                     /locus_tag="PKM23_06235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNY91107.1"
                     /translation="MDSKNGILNRETDFQEFVYQPQEERLFLNFGPSHPATHGTLRLV
                     LELDGERVVDCVPEIGYLHHGFEKLGEYRTYNQVVTITDRMNYVSPFNNNLAYILSVE
                     KLFGIEVSKRAQYLRVILAELGRIMDHAVCIGTHAMDLGAFTALLYLIRERENIYNLY
                     ELLTGTRLTVSYARVGGFARDRGIPADFMPRTLAVCDGLLKTVDEVDRLLSHNSIYLG
                     RTREVGIITPAAAADYGITGPCLRGSGVPFDLRQAAPYSGYEDFDFDIPVGSTGDVWD
                     RYMVRLEEMRQSVGIIRQAIANFPEGELYADSDSKVILPPKDDVYGSIEGLIHQFEII
                     MLNRGARAPRGEAYIPTEAPNGELGYHVISNEGNGPYRFRVRPPSFYNFQIIPSLLRG
                     RLLSDTVAILSSLNIIAGELDR"
     gene            complement(3192..3737)
                     /locus_tag="PKM23_06240"
     CDS             complement(3192..3737)
                     /locus_tag="PKM23_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNY91108.1"
                     /translation="MALQTNREQDVMASNQEIRELVLARFGERILAAEEECGQLALTV
                     SRDDHFELLKLLKESADLGYDILMDLFGVDYLEMGGRERYGVIYQLYSLGRNHRLRVR
                     VPVPEQDPRLDSAVPLWAAANWAEREVHDMYGITFSGHPNMTRILCPDDFSGFPLRKD
                     FPVQGIGYRENFEKIENPKTK"
     gene            complement(3737..4216)
                     /locus_tag="PKM23_06245"
     CDS             complement(3737..4216)
                     /locus_tag="PKM23_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNY91109.1"
                     /translation="MGIESKLGDGIITTTLDAVINWGRKNSLWPMPFGTACCAIEFMC
                     VLGPKYDMGRFGAEAMRFSPRQSDLLIVSGRVNIKMMHILQQIYRQMPDPKWVISMGA
                     CASSGGVFDTYSTIQGIDNFIPVDAYVPGCPPRPETVIDAVMKIQQKIQQERSREYR"
     gene            complement(4207..4572)
                     /gene="ndhC"
                     /locus_tag="PKM23_06250"
     CDS             complement(4207..4572)
                     /gene="ndhC"
                     /locus_tag="PKM23_06250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNY91110.1"
                     /translation="MLHDLMPILLLFAVSAILGVAILILTNLFGPSRPNPVKLSTYES
                     GKIPIGGPRIRFSIRFYIIAMLFVLFDIELVFFYPWAVVYRQYLSLGSFIFWEMILFI
                     GLLLVGYFYAWKKGALEWE"
     gene            5037..5237
                     /locus_tag="PKM23_06255"
     CDS             5037..5237
                     /locus_tag="PKM23_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNY91111.1"
                     /translation="METGTVKWFNASKGYGFITREQGGDVFVHFNAIQGTGYRTLDEG
                     DKVEFEVTQGAKGLQAEKVTKL"
     gene            complement(5311..6537)
                     /locus_tag="PKM23_06260"
     CDS             complement(5311..6537)
                     /locus_tag="PKM23_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNY91112.1"
                     /translation="MQKQTRLWTIEHEDLTIGPLSSYALALWGVQGLLYPNDVLVDGE
                     SNRCPAGYLEGMKPFIRNNGHLRRAKLWAIAGGKGGVGKSVLTTLLGVALARSGKRVV
                     IVDADFTGANQRQLFAMRQASPNIWRLVDSQEPLAGAALATSVPNLRLITAPETAHDS
                     DQTTILRRIRFIRALRHIDAEYVLLDFGPRTDLRELSYFLTADLNLVVSTPEPTAMEN
                     LAKFIKGVVKQKLQLAVDSLSRSQARGEWGVDEKATLLQQTVSHLHRHGIPAEEMMRR
                     VAGSFSLRVLFNQVTDEGYRKDMNLLNSYLAHEVGVSAEIGGAIPFDPAVRTAIRANR
                     LFALDPATPVMRRMDEIIHALCEIRNTEPPVYQLKETRAREIDSSVLLCGAWCPAWGE
                     CNFQNPGDVCPVKNMN"
     gene            complement(6911..8014)
                     /locus_tag="PKM23_06265"
     CDS             complement(6911..8014)
                     /locus_tag="PKM23_06265"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNY91113.1"
                     /translation="MTGKKVRRVGILTAGGDCPGLNAVIRAVAKSLMLNHKVEVIGIE
                     DGFEGLIEDRMRVLSYDDVSGILQRGGTILGTSNKADPFAQAILVDGKPVKKDVSAGT
                     IAHVEKWRLDALVCIGGDGSMSISRKLVERGVPVVGVPKTIDNDLFGTDVTFGFDTAA
                     SIATDAIDRIHTTAQSHHRVMLVEIMGRTAGWLTISAGIAGGADVIILPEMEYDLNCI
                     CEKVAERSRYGKRFSIIAVSEGAKPRGGEVVVSRLVKDSHESIRLGGVSIKLGNDIED
                     LTGLETRATVLGHLQRGGPPTPNDRILATRFGVGAAALVATGQFNRMVALRGTDIVSV
                     PITEVGGRTRTVPLDHPLLDVAEAVGTALGAKR"
     gene            complement(8083..9285)
                     /gene="alr"
                     /locus_tag="PKM23_06270"
     CDS             complement(8083..9285)
                     /gene="alr"
                     /locus_tag="PKM23_06270"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNY91114.1"
                     /translation="MIRVKEGGAMPPGEVFLRPSRVEVDLDAIGANYARIRDVLGEVK
                     LCAVVKADAYGAGAVMVARHLESLGIERLAVVTLDEAVELRQAGIRAPILNMGAIFPD
                     QAPLAVRYYIEQMVFQPELLGALDAAARAAGVLARIHLKVDTGMSRYGLPAEAAAALI
                     ARLPEWPALECIGVMTHFPMSDGLDKSFAQLQIGRFSALRREVEGAGRRIPLWHMCNS
                     GGVLDLPEAHMDMVRVGLMLYGYYPSREVRRPFDLRPALQVRTRIAAVRTISRGDSVG
                     YGRRYMAEQPERIAVLPVGYADGYDRKLRNIGEVLVAGRRAPIVGGLCMDACFIQVTD
                     IPEAVEGSEVTLMGSDGGEEISPHDIAARIESVSYEVMARWGRRLPRVYRRGGEIVAI
                     RSDLTAEN"
     gene            complement(9401..10114)
                     /locus_tag="PKM23_06275"
     CDS             complement(9401..10114)
                     /locus_tag="PKM23_06275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY91115.1"
                     /translation="MVPSSAQRTAYYQEVARYFDDEARLFERHYENNPILQRIRGDFR
                     TITLEQPFTSGLEIGCGPGIDIAWFAAAHPDLRWRAIDIAPRMVGEAREKLAALSGVD
                     VRVEVGAPEDLATLFPQERFDLIYCYFGALNTVPDLRETAQALGRALTPQGAMVLTFV
                     NRWFLFDLIWNLLRLRPRRALARITGSWAGYSPSRPLASSARSAREVRRAFAPNSGAL
                     RPGGIPSSTRPGTATVSCP"
     gene            complement(10114..11007)
                     /locus_tag="PKM23_06280"
     CDS             complement(10114..11007)
                     /locus_tag="PKM23_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91116.1"
                     /translation="MHTIESAILSTLLYFDIFDYPLSRDQLYFYLMYVKPERFEFERV
                     LDELLADGKICATAGWHHLPGHEALIHKRQEGENHARRLWSMAYEAAGRMRHLPFVRG
                     IYLSGDLSKGVAGHDSDIDFFIITARRRVWICKLFLAIFRRIGRNNPDKLLCFNYLLS
                     ESHLDLEERNLFTAAEVIGLAPLYGLRVFRRFLRHNEWVAGYFPRYRRAPKEEYCIYH
                     GHSRRQKWAEFFFRNPLADALEFLLPRIWRLAWGWKYRRQPQTRSALLKGIHHSYSKA
                     HGYPTDREVLQEYSRRLEEAS"
BASE COUNT         2222 a         3311 c         3565 g         2169 t
ORIGIN      
        1 ttccatctat cttgaaagtg ggcatgggtc cattctgctg gttagtggga ttccgttaac
       61 gccgctgcgg acagcggccg agttcggcat gggcggcgaa ctcgtcgcga aactgtttga
      121 gaaaactggc cgccggcatg gccgccgcat cgccgagggg acagatggtg cgaaaattga
      181 tatgccgggc gatatcctcc aggaggggca gatcctcggg gcggccgccc cccatttcga
      241 ggcgcttgag gatcttgacc atccaggtcg taccttcgcg gcagggggtg cactggccgc
      301 aggattcgtg ggcgtaaaaa cgctcgagat tgtagagggc attgaccatg caggcatcct
      361 cgtccatgac gatcaccccg gcggagccga gcatcgagcc ggcctcggcc agcgcttcat
      421 agtccatgct cacatcgatc tggtcggccc tgagcaccgg caccgaggag cccccgggga
      481 tgaccgcctt tagtttcttg ccgccgcgga tgcccccgca ttcctcgtag atcagctcct
      541 tgaggttttt gcccatcggg aattcgtaga ggccggggcg gttgacgtgg ccgctgacgc
      601 agtagagctt ggggccggtg ttcttgggat tgggcccgat ggcggcgaac cactcggccc
      661 cgcgggtgaa gatcaggggc aggttggcga gggtctcgac gttgttgacc acggtcgggc
      721 tcttgaaagc ccccaccacc gcggggaagg ggggtttcag gcgcggccag ccccgtttgc
      781 cctcaagcga ctcgagcagg ccggtttcct cgccgcaaat gtaggccccg gccccgcggt
      841 ggacggtcac ctgcagatcg tagctgctgc cgaggatttt ttccccgagc agattcttcg
      901 cctccgcctc ggcgatcgcc tcctccagca cctgcgcgcc atgggcgaat tcgccgcgga
      961 tgtagatgta ggcgctgtgg gcgttgatgg cgtacgaggc gatggcgatg ccctcgagga
     1021 gcatatgggg ttcatgctcg acaagatagc ggtccttgaa ggtgccgggc tcggactcgt
     1081 cggcgttgca gaccagatat ttcggttttt ccgagtcgcg cggcacaaag ctccatttca
     1141 tgccggtcgg aaagccggca ccgccgcgtc cgcgcagccc cgactttttg acttcttcga
     1201 tgacctcggc cggctgccac tgcctgacca ccttctccca cgccgaatag ccctggttgg
     1261 cgagataaaa ggccagactc ttcgattccg gcttgccgat gttgtggagg aaatatttct
     1321 cgctcatgcg cctctatcct gcttggtcag ggcaatttcg ccaaaatgtc gtcgatcttc
     1381 tcgacggtca gattctcata atagtcgtcg ttgatctgca tcaccggggc ggttccgcac
     1441 gagcccagac actccacctt cttgagggtg aactttttgt cgggggtggt tccgcccacc
     1501 ttgatgccca gcttctcctc gaggtggtcg accagagact cggcgccgag cagggcacag
     1561 ctgagggtgg cgcagacctg gatgacatag cgtccctcct cctggcgcgg atagagggtg
     1621 tagaaggaga ccacgtccat gacctggagg ggggagagcc ccagcagccc ggcgatgtat
     1681 tccatcacct ccggggtgat ggtcttgaac tcggctctgg cgatgtggag cacctgaagg
     1741 agcgccgcct ccttgtcagg atagtggccg agaagctttt ccactctctt ctgggcggtt
     1801 gcgctgaaag caatgctcat ctctggtcgt cctaagttcg tcgcgtggat ttcattctga
     1861 ccgtcatccc ggcactgcgc cgcggctgcg ggtgcacccg ggcggaagtg gaatctagcg
     1921 gtcgagttcg cccgcaatga tattcagact gctcagtatg gccacggtgt cggagagcag
     1981 gcggccgcgc agcaggctgg ggatgatctg gaaattgtag aatgagggag gccgcacgcg
     2041 gaagcggtag gggccgttgc cctcattgct gatgacgtga tagccgagtt cgccgttggg
     2101 ggcctcggtg gggatgtagg cctcgccgcg gggggcgcgg gcgccgcggt tgagcatgat
     2161 gatctcaaac tggtggatca gcccttcgat cgagccgtag acatcgtcct tggggggcag
     2221 gatcaccttg gagtccgaat cggcatagag ttcgccctcc ggaaagttgg caatggcctg
     2281 gcggatgatg cccaccgact ggcgcatctc ctcgaggcgc accatgtagc ggtcccagac
     2341 gtcgccggtg ctgccgacgg ggatatcaaa atcgaaatcc tcatagccgg agtagggggc
     2401 cgcctggcgc aggtcgaagg ggacgccgct gccgcgcagg caggggccgg tgatgccgta
     2461 atcggccgct gcggccgggg tgatgatgcc gacctcccgg gtgcgtccga gatagatcga
     2521 attgtgggag agcagccggt ccacctcatc gacggtcttg agcaggccgt cgcagaccgc
     2581 cagggtgcgc ggcataaaat ccgcggggat gccgcggtcg cgggcgaagc cgccgacgcg
     2641 ggcgtagctg acggtgaggc gggtgccggt gagcagctcg tagaggttgt agatattctc
     2701 gcgctcgcgg atcagataga gcaatgccgt gaaggcccca agatccatgg cgtgagtgcc
     2761 gatgcagacg gcgtgatcca tgatgcggcc gagttcggcg aggatgacgc ggagatactg
     2821 cgcccgcttg ctcacctcaa tgccgaagag cttttcgacc gagaggatgt aggcgaggtt
     2881 gttgttgaag ggcgagacgt aattcatgcg gtcggtgata gtgacgacct ggttataggt
     2941 gcggtactcg ccgagctttt caaagccgtg gtggagatag ccgatctcgg gaacgcagtc
     3001 aacgacgcgc tcgccgtcaa gctcgagaac cagtcgcagg gtgccatgag tggcgggatg
     3061 ggagggcccg aagttgagga acagccgttc ctcctggggc tggtagacga actcctggaa
     3121 atcggtctcg cggttcagga tcccgttttt gctgtccatg ctctttccgg tggttgtcaa
     3181 tccgcacctc tttacttcgt cttgggattt tctatctttt caaagttttc gcgatagccg
     3241 atgccctgaa ccgggaaatc cttgcgcagg gggaatccgc taaaatcatc cggacagagg
     3301 atgcgggtca tgttgggatg gccggagaag gtgatgccgt acatgtcgtg gacctcgcgc
     3361 tcggcccagt tggctgcagc ccagaggggg acagcgctgt cgaggcgggg atcctgctcg
     3421 ggcaccggaa cgcgcacgcg cagacggtgg ttgcggccga ggctgtagag ctgatagatc
     3481 actccgtagc gctcccgccc gcccatctcc agataatcga ccccgaagag atccataaga
     3541 atatcatacc ccaggtccgc gctctccttg agcaacttga gcagttcaaa atgatcatcg
     3601 cggctgaccg tgagggcgag ctgtccgcac tcctcctcag cggcgagtat cctctcgccg
     3661 aagcgggcga gaacaagttc cctgatttcc tggttgctgg ccataacgtc ctgttcacga
     3721 ttggtttgca gtgccatcaa cggtactcgc gcgaccgttc ctgttggatt ttctgctgga
     3781 tcttcatcac ggcgtcgatc accgtctcgg gtcgcggcgg gcagcccggc acgtaggcat
     3841 cgacgggaat gaaattgtca atgccctgga tggtcgaata ggtgtcgaac accccgcccg
     3901 aagaggcgca ggcccccatc gagatcaccc acttgggatc gggcatctga cggtagatct
     3961 gctggaggat gtgcatcatc ttgatgttga cgcgcccgga aacgatgagc agatcggact
     4021 ggcgcggcga gaatcgcatc gcctcggccc cgaaacggcc catgtcatat ttgggaccga
     4081 gcacgcacat gaactcgatc gcgcagcagg cggtgccaaa gggcatgggc cagagcgagt
     4141 tcttgcggcc ccagttgatc acggcgtcga gggtggtggt gatgatgcca tcaccgagct
     4201 tgctctctat tcccattcca gggctccttt tttccaggcg tagaaatagc cgaccagcag
     4261 caggccgata aagaggatca tctcccagaa gataaagctg cccagggaga gatactgccg
     4321 gtataccacc gcccaggggt agaagaaaac cagctcgatg tcgaaaagga cgaagagcat
     4381 ggcgatgatg tagaatctga tggaaaagcg gatacgcggg ccgccgatcg ggatcttgcc
     4441 cgattcatag gttgaaagct tgacgggatt gggacggctg ggtccgaaga gattggtgag
     4501 gatgaggatg gcgacgccaa gaatcgcgga gacggcgaaa aggagcagga tcggcattag
     4561 atcatggagc atgcgatgcc tctcgtttat ggctgatcat gattgcagga aggctgctcc
     4621 gcgcggggag cgcgggccgg cgcacagctg tggtgagccg aggctgttcc cggtttcgat
     4681 gcaaataaaa taagccgatg aatttcaaaa agcaagctaa ttatttgctc tccctcctcc
     4741 cccccctctt cctgcgcgtc gactgcacgt gcccggcagc cggcagctgc cggcggaaaa
     4801 aaaatgaaat taattcaaaa aatgcttgca tttttattga aattattgta taataatgta
     4861 tcaataatgt acgcttgtgc ccgccttcca aaatcggtaa cggagtttgt taaacgacag
     4921 atccaaagaa tcgtgtagaa tggcatcaag aggctattca cgcgattttt ttatgccctg
     4981 gcgagacgag tagagttgta cacagctgtt cattttttta agcaaggagt ttccagatgg
     5041 aaacaggaac tgtcaagtgg tttaacgcct ccaaaggcta cggctttatc acccgtgagc
     5101 agggcggcga tgtcttcgtc catttcaacg ccattcaggg caccggctac cggacgctcg
     5161 atgagggcga caaggtggag ttcgaagtca cccagggcgc aaaaggcctt caggctgaaa
     5221 aagtcaccaa actgtaagct tgacataaaa gtcacaaaaa aaaatcccgg tccgtatgga
     5281 ccgggatttt tttttgcctg caggcgctgt tcaattcatg ttcttgaccg gacagacatc
     5341 cccgggattc tgaaagttgc actcccccca ggccggacac caggccccgc agagcagcac
     5401 cgaggagtcg atctcgcgcg cccgcgtctc cttgagttga taaacggggg gctctgtatt
     5461 cctgatctcg cacagggcgt ggatgatctc gtccatccgc cgcatcaccg gcgtggccgg
     5521 atcgagggcg aagagacggt tggcgcggat ggctgtgcgc accgccggat caaaggggat
     5581 ggctccgccg atctccgcac tcacccccac ctcatgcgcc agatagctgt tgagcaggtt
     5641 catatccttg cgatagccct catcggtgac ctgattgaag agaacccgca ggctgaaact
     5701 ccccgccacc cgccgcatca tctcctcggc cggaatcccg tgccggtgca gatggctgac
     5761 ggtctgctgg agaagggtcg ccttctcatc aacaccccac tcccccctcg cctggctgcg
     5821 gctgaggctg tccactgcca gctgcagttt ttgcttgacc acccccttga tgaatttggc
     5881 caggttctcc atcgccgtcg gttcgggagt ggagaccacc agattcagat cggcggtgag
     5941 gaaataggag agctcgcgca gatcggtgcg cggtccgaaa tcgagcagga catattcggc
     6001 atcgatatgg cgcagggcgc gaatgaagcg gatgcgccgc aggatggtgg tttgatctga
     6061 atcatgggcg gtttcgggcg cggtgatcag gcgcagattg ggcaccgagg tggccagcgc
     6121 cgcacctgcc agcggctcct ggctgtccac cagccgccag atattgggag aggcctggcg
     6181 catggcgaaa agctgacgct gattggctcc ggtaaaatcg gcatcgacga tgacgacgcg
     6241 cttgcccgag cgcgccagcg cgaccccaag cagggtggtg aggaccgatt tccccactcc
     6301 tcccttgccg ccggcgatag cccagagctt ggcgcggcgg agatgaccat tgttgcggat
     6361 gaagggcttc atgccctcca gatatccggc tggacagcgg ttggactcgc cgtcgaccag
     6421 cacgtcgttg ggatagagaa gcccctgtac tccccaaagg gcaagggcat atgaactcag
     6481 gggaccgatg gtcaggtcct cgtgttcaat ggtccagagt ctggtttgct tttgcatatt
     6541 tgcccgtctg gttttgcacc atgatgatac aatgtactgc tgcagtgcat tcccccggcg
     6601 gatcggcggc acgcgccccg gcgccggggt ttcagctgcg ttcaggtaaa gcaaactctt
     6661 gtaattccat aaccattatt tcatgcgggg gctgaaatgg gtagtggaaa ccagtttcgc
     6721 ttttgtacct ggatatgcaa agtccatacc aaagatgcag ggcagaggag gaagggcaat
     6781 ccccgctcct tcgctcccgg tgaggggcgc agagagaaag cggagagcag atgatgcaga
     6841 tagaatacca ggcggcgggg ccgccgccgg tgtgtatgac cggcggcgga atccgctgag
     6901 gctgaacgag ctatctttta gcgccgaggg cggtgcccac ggcttcggcg acatcaagca
     6961 ggggatggtc gagcggcacc gtgcgcgtcc tgccccccac ctcggtgata ggcaccgaga
     7021 cgatgtcggt gccgcgcagg gccaccatgc gattgaactg gccggtggcg acgagcgcgg
     7081 ccgcacccac gccaaaacgg gtggcgagga tgcggtcgtt gggcgtcggc ggaccgccgc
     7141 gctgcaaatg gccaagcacg gtggcgcggg tctccagacc ggtgagatcc tcaatgtcat
     7201 tacccagttt tatcgagacc ccgccgaggc ggatcgattc atgactgtcc ttgaccagac
     7261 ggctgaccac cacctccccc ccgcggggct tggcgccttc ggagaccgcg atgatgctga
     7321 aacgcttgcc gtagcggctg cgctcggcca ccttttcgca gatgcaattg aggtcgtact
     7381 ccatctccgg caggatgatc acatccgccc cgccggcgat gcccgccgag attgtcagcc
     7441 agccggccgt gcggcccatg atctccacca gcatgacgcg atgatgcgac tgggctgtgg
     7501 tatgaatgcg gtcgatggcg tcggtcgcga tcgaggcggc ggtgtcgaag ccgaaggtga
     7561 catcggtccc gaagagatcg ttgtcgatgg tcttgggcac gccgacgacc ggcacacccc
     7621 gttcgaccag cttgcgcgat atgctcatcg aaccgtcgcc cccgatgcag accagggcat
     7681 ccagccgcca tttctcgacg tgggcgatgg tcccggccga gacgtccttt ttgaccggct
     7741 tgccatcgac cagaatcgcc tgggcaaagg gatccgcctt gtttgaggtg ccaaggatgg
     7801 tgcccccacg ctggagaatg ccggagacat cgtcgtagct caggacgcgc atgcggtcct
     7861 cgatcagccc ctcaaagccg tcttcgatgc cgatgacctc aaccttgtgg ttgagcatga
     7921 gggatttggc gaccgcgcga atgaccgcgt tgagccctgg acaatcaccg ccggcggtga
     7981 ggatgcccac ccggcgcact ttcttgccag tcatatcaag tactcccgtc tgaaggtaaa
     8041 caactgctgc agtatacgct aaaaaaaagc accaatttca attcaattct ccgccgtcag
     8101 atcgctgcgg atggcgacaa tctctccgcc ccggcggtaa acacgaggca gccggcgccc
     8161 ccagcgcgcc atcacctcgt aggagaccga ttcaatcctg gccgcgatat cgtgcggcga
     8221 aatctcctcc cccccgtcgc tccccatcag ggtgacctcg ctgccctcca ccgcctccgg
     8281 aatatcagtg acttgaatga aacaggcgtc catgcagagc ccgccgacga tgggtgctct
     8341 ccgccccgcc accagcacct cgccgatgtt gcgcagtttg cggtcatagc cgtcggcata
     8401 gccgaccggc agcaccgcga tccgctccgg ctgctccgcc atgtagcgcc ggccatagcc
     8461 gacgctgtca ccccggctga tggtgcgcac ggcggcgatg cgggtgcgga cctgcagggc
     8521 ggggcgcagg tcaaagggcc gccgcacctc ccgtgaggga taatagccat agagcatcag
     8581 tccgacccgg accatatcca tatgggcctc gggcagatcc agaacccccc cgctgttgca
     8641 catgtgccag agcgggatac gccgccctgc gccctcgacc tcgcgccgca gtgcggaaaa
     8701 acgcccgatc tgcagctggg cgaagctctt gtccaggccg tcggacatgg gaaaatgggt
     8761 catgacgccg atgcattcga gcgcgggcca ttccggcagg cgggcaatga gagccgcggc
     8821 cgcctcggcc ggcaggccgt agcggctcat gccggtatct accttgaggt ggatcctggc
     8881 cagcacaccc gccgcccggg ccgccgcatc gagggcccca agcagctccg gctgaaaaac
     8941 catctgctcg atataatagc gtacagccag aggggcctgg tcgggaaaga tcgcccccat
     9001 gttaagaatc ggggcgcgaa tgcccgcctg acgcagctcg accgcttcat cgagggtcac
     9061 caccgccaac cgttcgatcc ccagggattc gaggtggcgc gccaccatca ccgcacccgc
     9121 gccgtaggcg tcggccttga cgaccgcgca gagctttacc tcccccagga cgtccctgat
     9181 ccgggcgtag ttggcgccga tggcatccag atccacctcg acccgcgaag gccggagaaa
     9241 aacctccccg ggcggcatgg cgcccccttc cttcacccgg atcacttttt ggcctcctcc
     9301 cttctcatga agagatagag ggtatactcc cccagattcc acaagggggt gcggttgagc
     9361 agggcgtcga tgcgccagag caggcggccc agccacccct ctacgggcag gaaacggtgg
     9421 cggtaccagg ccgggtagac gatggaatac cccctggcct taatgcaccg gaattcgggg
     9481 cgaaggcgcg gcgcacctcg cgggcggagc gggcggaact ggccagtggg cgggatgggg
     9541 aatagcccgc ccagcttccg gtgatgcgcg ccagggcccg gcgcggccgc aggcgcagca
     9601 ggttccagat gaggtcgaag agaaaccagc ggttgacaaa ggtcagcacc atcgccccct
     9661 gcggcgtcag ggcgcgcccg agggcctgtg ccgtctcgcg caggtcgggt accgtattga
     9721 gggccccgaa ataacagtag atcagatcga agcgctcctg cgggaagagg gtggcgagat
     9781 cttccggagc gcccacctcg acgcggacat ccaccccgga cagagcagcg agcttttccc
     9841 gcgcctcgcc gaccatgcgg ggggcgatat cgatggcgcg ccaacgcagg tccggatggg
     9901 cggccgcaaa ccaggcgatg tcgatgcccg gtccacagcc gatctcgagt cccgatgtga
     9961 agggctgctc cagggtgatg gtgcggaaat caccgcgaat gcgctggagg atggggttgt
    10021 tctcataatg gcgctcgaag aggcgagcct cgtcgtcgaa atagcgcgcc acctcctgat
    10081 aataggcggt cctctgtgcg gaggagggta ccatcaggag gcctcctcca ggcgccgtga
    10141 gtactcctgc agcacctcac ggtcggtcgg ataaccatgg gccttgctat aggagtgatg
    10201 aatacccttg agcagtgcag accgggtctg tggctggcgg cgatatttcc agccccaggc
    10261 cagccgccag atgcgcggga ggagaaactc cagggcatcg gcgaggggat tgcgaaagaa
    10321 gaactctgcc cacttttgtc tgcggctgtg gccgtggtag atgcaatact cctccttggg
    10381 cgcccggcga tagcgaggga aatagcccgc cacccattcg ttgtggcgga gaaagcggcg
    10441 gaagacccgc agtccgtaga ggggggcgag gccgatcacc tcggcggcgg tgaagagatt
    10501 cctctcttcg aggtcgaggt ggctttctga gaggagataa ttgaagcaga gcagcttgtc
    10561 cgggttgttg cggccgatgc gccggaagat agcgagaaag agcttgcaga tccacaccct
    10621 gcgccgggcg gtgatgatga aaaaatcgat gtcgctgtca tggccggcca cccccttgga
    10681 gaggtcgccg gagagataga tgccgcgcac gaaagggaga tggcgcatcc ttcccgccgc
    10741 ctcataggcc atgctccaga ggcggcgcgc atggttctct ccctcctgcc ttttatggat
    10801 cagcgcctcg tgtccgggca gatggtgcca gcccgccgtg gcgcagatct tgccatcggc
    10861 caggagttcg tcgagtacgc gttcgaactc aaaccgctcc ggtttgacgt acatcagata
    10921 aaaatagagc tggtccctgc tcaggggata atcgaagatg tcgaagtaga ggagcgtcga
    10981 caggatggcc gattctatgg tatgcacaac ttcctttcaa gatccgaatc tgagtggagc
    11041 ttcggccggc gacttgcgca attccgcgat cgccgcccgt tttacccccc cgaatatata
    11101 ataaaattgg aaaaaagtaa ataaattccc atattatttc cggttttacc cacttttttt
    11161 tcaacgggct gccgggcaca aaggaaggat ggcgggctgc gcatcagccg ctgcagcccg
    11221 tttgaaactt tttaccccag tgttcgtctt tatactgtct ttaagtg
//
LOCUS       DAOPWU010000087        11188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_5909_length_11188_cov_5.676278, whole genome shotgun sequence.
ACCESSION   DAOPWU010000087 DAOPWU010000000
VERSION     DAOPWU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5909_length_11188_cov_5.676278"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PKM23_06285"
     CDS             complement(<1..526)
                     /locus_tag="PKM23_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91117.1"
                     /translation="MYYRSMWFRFILLYAVITLLPGGSLIARQGSVIQERALPGVVSL
                     ALREPLPDGAALRRAALPGLEHTLQSLQIHTLAPVAPAPRAVLPKIGHRTRIDNLYNA
                     WYAGGRPPEEVAKILAGDPAVEFAEARYYYTLTAMPDDPLAGKQWYLDAVQLPRAWEI
                     AKAEQGGVVIAIIDG"
     gene            complement(682..1635)
                     /locus_tag="PKM23_06290"
     CDS             complement(682..1635)
                     /locus_tag="PKM23_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91118.1"
                     /translation="MQIQLLAPFERAFNRMKTALFKPFDLRKWLIIGFTAFLADLLDG
                     GGGGGRGMQNQSHGDWGAVLAAPYQALEWLQSKPEWMTLLIFILLFAAALVLLLLWLS
                     SRGKFMFLDNVVQQRALVSHPWHHYRQPAASLFRFRALFALIILGLAGGTLYHLWRMA
                     YTRWNESGDLWQLLPSLALWILFLLLIILTFSYVKLLLDHFVVPLMYKHNLGCVQAWE
                     KFMPLHWAHVGSFILYALFILIAIIALVALVVIVGLFTCCVGFVILAIPYLNSVLMLP
                     FSYWLRSFSLEYLAQFGSEYNLLEEAGREEVNPYPEPPTVA"
     gene            1985..3448
                     /locus_tag="PKM23_06295"
     CDS             1985..3448
                     /locus_tag="PKM23_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91119.1"
                     /translation="MRRYAIPLLLFLLICFQRTTHAQEKFLFVRQAGADEVHIAIDPQ
                     VHIDYGFSYPLTFALELSAFEEGARAWYRYRQGGAWLQLPEKSGGDFFNGIAAVRFER
                     GSSTAYLSLGFSAESDSIFLRITNAGRKSIPARFNRICTYYDDREMAVTASADDMAGW
                     SRAKFVRTLHITRSYRLWVSCGINSGGADAAVYRYIQAQLDSGYVEAACHSRSHPSLP
                     YKDYPGEITGNKEDIIRNLTLPAAFTSGEREYVYTWIAPNGRTNAIIDSLLTLNKFLF
                     NRLYVSNFFGLSPWNEKIHMFTNAGVTRAFDPPASQLGWGIGTNDLGSLNGAFDKAMS
                     EGSVYHVMCHPNVVEWEKGYPVQHLAYICNRPNVWYVAVGHLYLYHLAQSSYQVDSAA
                     FAGAEEQPEDFILQQNYPNPFNPETTIAYRLAGTGRVRLELYNSRGEMVERLVDGLQN
                     PGHYRVVWNAGGRAAGVYFCRLISGAGTETRKMLLVR"
     gene            3477..6023
                     /locus_tag="PKM23_06300"
     CDS             3477..6023
                     /locus_tag="PKM23_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91120.1"
                     /translation="MRWFGCLLTAMLMAAASNLQGEGEPPSWKTAKLPRLDERVSFTS
                     TAHTVHDFFIDDFDGDGLEELCELRTDQEHPLFLSIRELYQVGYTFPGQPVTLHHEAY
                     TLFPLQGSSRFNFTTYRIAGEKGWIDLYNRRAEKVDSVATVAGRDASGDGLWTGSLNS
                     AALCDINRDDRPDLLIHLNTGADRQPRTLLAYDLATKATLLDLSFAPMLTQVTPADLD
                     GDGGMELVITLGGASDGPFFGPFSRDSSYVAVLTGDGRILAKRAFGGESSYVNVKLAD
                     LDRDLLPEIIAAPFSLISAHSQQPRLYALDGLSLAVKSELDDPRGMAPIYSFLVEDMD
                     LDGTPEIIAGDLANHAIVLRADAERGRLRITALAHAGGGGQPLLVEDVDRDGRAELFF
                     GTQSPLALWLVDSALRPLAWMPVAKTRPSRIEALALINASQRSFCLLDQGVLKVLTLP
                     VDALFPRGQERVHIPGSRAGIHPIALLLLTVLLLLLLAGALYLVFRLRHTLRNAGRFQ
                     SRRIGVALVDGGGRFLFCNPYFLRVIGREEERTIEHPVEEVMGTAPLTGLLAFYKAFK
                     RENESYQQQEFDLEIEGRAQNFAVELVRHQRLQNHIVLLLADLSESLQKERLRIWAAM
                     AMRMAHKTKTPLATVLLAIQRLQRAYRKFSPDYAPEYEEMTKTAIHEIERVRDSINAF
                     MKFARLDPPVFLPDDFSRVIQESLQEYLPRIPDTVQVRTRFETAELPVHIDAGQFKEA
                     LYNLLDNAIAAIQGEGVLSITTMLEKNPLYPQGEKDQALLEITDNGVGISAEELSHIF
                     KTGFTTSASGTGMGLPLARSILESLGGTIEIDSRKMSGTTVFVRLPIRSV"
     gene            6037..7389
                     /locus_tag="PKM23_06305"
     CDS             6037..7389
                     /locus_tag="PKM23_06305"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY91121.1"
                     /translation="MKRIKGRVLIAEDDSAFAAQLASVLEGEDYQVVLARDGNEAVRQ
                     IAGGELDIGFIDLAMPGLDGMQVLEKAVGIAPDLPLVVITGHASIDRAIQATRLGAFD
                     FLEKPVNLERVLLTVERALGWRQLEQKNNWMAAEIMNRYRMVGTSPGMQDIYARIDQV
                     APTESTVLITGETGTGKELVAMAIHLRSHRAAGPFMRVNCAAIPDTLIESELFGHRKG
                     AFTGATENHDGKFMQAAGGSLFLDEIGDLSQPAQAKLLRVLQSREVEAVGAARPQPVD
                     VRIIAATNKNLTECISKGLFREDLYYRVCVIDINLPPLRERKEDIPELSGHFLNYFCG
                     QYNRRLEGFTPAALQTLMNYHWPGNVRQLRSCIERAVILARGESLSEADLRLMMSGKP
                     VNEEAPATLRQARERFEKSYIEQVLKSHDWKMEESAATLGIDRTNLYKKMQKLGIHRE
                     "
     gene            complement(7825..8631)
                     /locus_tag="PKM23_06310"
     CDS             complement(7825..8631)
                     /locus_tag="PKM23_06310"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNY91122.1"
                     /translation="MKIKITASLLLAAALALLACAGSRDTGKLAFVSIEEEIVLGREV
                     SVQAGQLLKIVRNQEVGQYLNQLAAEIGPHSHWSGLTYSVHLVNEPDLNHFSLPGGQI
                     FIYRGLFETLGSTDQVAAVIAHEIAHIAARDGVERLSAKYGWAVAAQSVIGQNPEIAR
                     QLFLNLYTKDTILDYPKPVEREADRKCATYLVKAGFDPAGLPGMVRLLRQAEKSHPGL
                     VKLLRKTHASASIRYRNVSSYVKTLDSPSPNPHPLADFLLIREILTKIPY"
     gene            complement(8628..10625)
                     /gene="ligA"
                     /locus_tag="PKM23_06315"
     CDS             complement(8628..10625)
                     /gene="ligA"
                     /locus_tag="PKM23_06315"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNY91123.1"
                     /translation="MSSRPEERIRELRDLIHYHNTRYYGLDDPEISDAEFDRLMRELV
                     ELEQRFPEFVTADSPTQRIGGAPLEAFPPALHTVPMLSLENALSEEELLAFDQRTRRA
                     LGSEEEIDYVCEPKLDGLAVELVYIDGLFTLGSTRGDGYTGEEITRNLRTIKSIPLKL
                     NNAPPGRLEVRGEVILGIREFETLNRERQERGESLFANPRNAAAGSLRQLDALITAGR
                     PLAIFCYGIGQAEALPFTTHAEMLESFRSMGLRVNPLIERVRGAGAVLAYHRAMGERR
                     GQLPYEIDGIVVKVDRVSQQEELGARSRSPRWAIAFKFPARQEVTRVLNIQVQVGRTG
                     TLTPVAILQPVQVGGVSVSRATLHNQDEIDRKDIRIGDWVVVQRAGDVIPEIVKVVES
                     RRTGDERPFLLPEACPVCGSHTVRPEDEAARRCINLSCPAQVKERIFHFASKRAMDID
                     GLGEKLVDQLVENGLVRDVADLYALTRAQLAGLERMAEKSAANIIAAIEASRSRPLER
                     LLFALGIRHVGEHVARLLIEAFGTMERLMSAHSEELMQVHEIGPQVAESVTDFFTTPE
                     NGRVIERLRQAGVEMAPSRAQRGDLFAGKTLVLTGTLSSYTREEAEKLILALGGRPAS
                     SVSSKTSYVIAGEAAGSKLDKARALGIPVLTEAEFKEMIKA"
     gene            complement(10628..>11188)
                     /locus_tag="PKM23_06320"
     CDS             complement(10628..>11188)
                     /locus_tag="PKM23_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNY91124.1"
                     /translation="KADRLGIFVRIDMEDSAVTSETIELFLRINDEFKRTGIVIQAYL
                     RRSLADVVRLSAHKTNYRICKGIYIEPRKVAFKDKQTVNDNFGLLVDTAFGMGSYVGI
                     ATHDERLYWKGLELIHKHKLTTGQYEYQMLLGVDEELRDIIVQAGHKLRVYVPFGKNW
                     YAYCVRRLKENPMMAWYIVKAMFGLR"
BASE COUNT         2200 a         3264 c         3545 g         2179 t
ORIGIN      
        1 cgccatcgat aatggcgatc acgactccgc cctgttccgc tttggcaatc tcccaggccc
       61 gggggagctg gacggcatcg agataccact gtttgccggc gaggggatcg tcgggcatgg
      121 cagtcagggt ataatagtat ctggcttcag cgaattcgac ggccggatcc ccggccaata
      181 ttttcgccac ctcctcgggc gggcggccgc cggcatacca ggcattgtac agattgtcga
      241 tgcgggtgcg gtggccgatt ttaggcagga cagcgcgggg tgccggggcg accggcgcga
      301 gggtatgaat ctgcagcgac tgcagggtgt gttccaggcc ggggagggcg gcacggcgca
      361 gggctgcgcc gtcggggagg ggttccctga gggccaggga gaccactccg ggaagggccc
      421 gttcctggat aacactcccc tgccgggcga tgagagaccc gcccggcaga agggtgatga
      481 cggcgtaaag cagaataaaa cggaaccaca tgctgcggta gtacatggcg gttttcccat
      541 ctgatcatga taatgtctat cttacgaaaa tacagcctga atcgcaagtt ttatctgtgc
      601 agatggaaca ccgcgcgtca tagtaaaagt tccctgcgcc gcccattcct tgagggctga
      661 accgcagttt gcggctgctg gtcaggcgac cgtcggcggc tcggggtagg gattcacctc
      721 ctcccgtccg gcttcctcca ggaggttgta ctcgctgccg aactgggcga ggtactccag
      781 actgaaagag cgcagccagt agctgaaggg cagcatcaac acggagttga ggtaggggat
      841 agccagaata acaaagccga cacagcaggt gaagagtccg acaatgacga cgagtgcgac
      901 cagggcgatg atggcgatca ggataaagag ggcgtagaga atgaagctgc cgacgtgtgc
      961 ccagtgcaga ggcatgaatt tttcccaggc ctgcacgcag cctagattgt gtttatacat
     1021 caggggcacg acaaagtggt cgaggagcag cttgacatag ctgaaggtca ggatgatgag
     1081 cagcaggaag agaatccaga gcgcgagcga ggggagaagc tgccagaggt cgccggattc
     1141 gttccagcgg gtataggcca tccgccagag atgatagagg gttcctcccg ccagaccaag
     1201 aatgatgagg gcgaagaggg cgcggaagcg gaaaagcgag gcggctggtt ggcgatagtg
     1261 atgccaggga tgggagacca gggcgcgttg ctgaaccaca ttgtcgagga acatgaattt
     1321 gcctctggaa ctcagccaga ggaggagcag tacgagtgcc gccgcgaaga gcagaatgaa
     1381 gatgagcagg gtcatccatt ccggtttgct ttgcagccac tccagggcct gatagggtgc
     1441 ggccagcact gcgccccaat cgccatggct ctgattttgc attccccggc ctcccccgcc
     1501 cccgccgtcg agcagatccg cgaggaaggc ggtgaagccg atgatcagcc acttgcgcag
     1561 gtcgaagggc ttgaaaagcg ccgttttcat gcggttgaag gctctttcga aaggcgccaa
     1621 gagttggatc tgcatagctc ctcctttttt tctcagtata ctgcctctgt atcaaaaagt
     1681 aacaactttt caccacaagg gcaagaaaaa gctggctatc ccccaaaatc tcggtcccac
     1741 ccagaggtaa ttaaataaaa aattgtattt tacctatcag aaacaccttg taaaaaaatg
     1801 cctcatgacg agggcacagg ccgcgagtat attggaaata gtatagtaag gagggattgg
     1861 ggacaagcga cgccaggtgt cgggtgggat ccagatttct gggggtacct tgcaagaaaa
     1921 agcggttgca atattgggaa aaaatagtag cttattatac tcacctcaag accacggaga
     1981 agtcatgcga cgctacgcca ttcctttact gctattcttg ctcatttgtt tccagcgcac
     2041 cacccatgct caggaaaagt ttctcttcgt ccgtcaagcc ggagcggatg aggtgcatat
     2101 cgccatcgat ccacaggtcc atatcgatta tgggttcagc tatcccctga cctttgccct
     2161 ggagctctcc gcttttgagg agggggcgcg ggcatggtat cgttatcgtc agggcggagc
     2221 ctggctgcag cttccagaaa agagcggagg cgattttttc aacggcatcg cagccgtgcg
     2281 ttttgagcgc gggagttcca ccgcctacct ctccctcggc ttttcggccg agagcgattc
     2341 gatttttctc aggatcacca atgccgggcg caagagcatc cccgcccgct tcaaccgcat
     2401 ctgcacctac tacgacgatc gggagatggc cgtcaccgcc agcgcggacg acatggcggg
     2461 ctggagcagg gccaagttcg tgcgcaccct gcacatcacc cgctcctacc ggctctgggt
     2521 cagctgcggc atcaattcgg gcggggccga tgccgcggtt taccgctaca tccaggcgca
     2581 gctcgactcg ggctatgtcg aagccgcctg ccattcccgc tcccatccct cccttcccta
     2641 caaggattac ccgggcgaga tcaccggcaa caaggaggat atcatccgga acctcacact
     2701 gccggctgca ttcacctcgg gcgagcggga atatgtctac acctggatcg cgcccaacgg
     2761 gcgcaccaac gccatcatcg attccctcct gaccctcaac aagttcctct tcaaccgcct
     2821 ttacgtcagc aattttttcg gtctgtcgcc atggaacgaa aagatccaca tgttcaccaa
     2881 cgccggggtg acgcgggcct ttgatccccc ggcttcgcag ctcggctggg gcatcggcac
     2941 caacgacctc ggcagcctca acggggcctt cgacaaggcc atgtccgagg gcagcgtcta
     3001 ccacgtcatg tgccacccca atgtggtcga atgggaaaag ggctaccccg tgcagcacct
     3061 tgcctacatt tgcaaccggc ccaatgtctg gtatgtggcg gtcggtcatc tctatctcta
     3121 tcacctggcg caaagctcgt accaggtcga ttcggcggct ttcgccggag cggaagagca
     3181 gcccgaggat tttatcctgc agcagaatta tcccaatccc ttcaatcccg agaccaccat
     3241 cgcctatcgt ctggccggga ccggaagggt gcgcctcgag ctttataaca gccggggcga
     3301 gatggtggaa cggctggtgg atggcttgca gaatccgggc cattaccgtg tcgtctggaa
     3361 cgccggcggc agggctgccg gggtctattt ctgccgcctg atcagcggcg cggggacaga
     3421 aacccgcaag atgcttctgg tgcggtaacc agccggttcg gccgggagat gaaaaaatgc
     3481 gatggttcgg ttgcctgtta accgccatgc tgatggcggc ggccagcaat ttgcagggag
     3541 agggggaacc gccgagctgg aaaacagcga agctgccccg gctggacgaa agggtgtcgt
     3601 tcaccagcac tgctcatacc gtgcatgatt ttttcatcga tgatttcgac ggtgacgggc
     3661 ttgaggagct ctgcgagctg cgcactgacc aggagcaccc tcttttcctc tccatccgcg
     3721 agctctatca ggtgggctat acttttcccg gccagccggt caccctgcac catgaggcct
     3781 ataccctctt cccgctgcag ggcagcagtc gcttcaactt taccacctat cggattgccg
     3841 gggagaaggg gtggatcgat ctttacaacc gccgggctga gaaggtcgat tctgtggcga
     3901 ccgtcgccgg gcgggatgcc tccggcgacg gcctctggac cggcagcctg aactcagcgg
     3961 cgctttgtga tatcaaccgc gacgaccggc cggatctgct catccatctc aacaccggcg
     4021 ccgatcggca accgcgcacc ctcctcgcct atgatctggc gaccaaggcg accctcctcg
     4081 acctctcctt cgcccccatg ctcacacagg tcacaccggc cgacctggat ggagacggcg
     4141 gtatggagct ggtcatcacc ctgggcgggg cttcggacgg tccttttttc ggtcccttca
     4201 gccgcgacag cagctatgtg gccgtcctga ccggcgatgg gcggattttg gccaaaaggg
     4261 ccttcggggg tgaatcttct tacgtcaacg tcaaactcgc cgatctggac cgcgatcttt
     4321 tgccggagat catcgccgcc cccttttcgt tgatcagcgc gcacagtcaa caaccgcgtc
     4381 tttatgccct tgatggcctc tccctggcgg tcaagagtga gctggatgat ccgcgcggga
     4441 tggccccgat ttacagtttt ctggtcgagg atatggatct cgacggcacg ccggagatca
     4501 tcgccggcga ccttgccaac catgccatag tcctgcgcgc tgatgccgag cggggccggc
     4561 tgcgcataac cgcccttgcc cacgccgggg gcggcggcca gccgctgctg gtcgaggatg
     4621 tcgaccgcga tggccgggcc gaactctttt ttggcacaca atccccactc gccctttggc
     4681 tcgtcgacag cgccctgcgg cccctggcct ggatgccggt ggcgaagacc aggccgtcca
     4741 ggatcgaggc tctggccttg atcaacgcct cgcagaggag tttctgcctc ctcgaccagg
     4801 gagtgctcaa ggtgctgacc ctcccggtgg atgccctttt cccgcgtggg caggagcgcg
     4861 tgcacattcc cggcagcagg gcggggatcc atccgatcgc gctgctcctc ctgaccgtcc
     4921 tgctcctgct cctcctggcc ggcgccctct atcttgtttt caggctgagg cacacactgc
     4981 gcaacgcggg ccgcttccaa tcccgccgca tcggagtggc cctcgtcgac gggggaggcc
     5041 gctttctctt ctgtaatcct tatttcctga gggtgatcgg gcgggaagag gagaggacca
     5101 tcgaacaccc cgttgaggag gtgatgggga ctgcccctct gaccggtctg ctcgcttttt
     5161 ataaggcctt taaaagagag aatgagagct accagcagca ggaattcgac ctcgagatcg
     5221 aggggcgggc gcagaatttc gctgtggaac tggtccgcca tcaacggctg cagaaccata
     5281 tcgtgctgct gctggccgat ctttcggaga gcctgcagaa ggagaggctg aggatctggg
     5341 cggcgatggc gatgcgcatg gcccacaaga ccaaaacccc gctggcgacg gtcctgctcg
     5401 ccattcagag acttcagcgc gcctaccgca aattcagccc tgattacgcg ccggagtatg
     5461 aggagatgac caagacggcg atacacgaga tcgagcgggt gcgggattcg atcaatgcct
     5521 ttatgaagtt cgcccggctc gatcccccgg tctttctccc cgatgatttt tcccgcgtca
     5581 ttcaggagtc tctgcaggag tatctgccgc gcatccccga tacagtccag gtgcgcaccc
     5641 ggtttgagac cgcagagctg ccggtccaca ttgatgccgg ccaattcaag gaggccctct
     5701 acaatctgct cgacaacgcc atcgccgcca tccagggcga aggagtactg tcgatcacga
     5761 ccatgctgga aaaaaatccc ctctacccgc aaggagagaa ggatcaggcc ctcctggaga
     5821 tcaccgacaa cggggtgggc atctccgccg aagagctttc ccacatcttc aagacaggat
     5881 tcaccacttc ggcctcgggc acgggtatgg gactccctct ggccaggagc atccttgaat
     5941 ccctgggcgg caccatcgag atcgacagcc gtaaaatgag cggtaccacg gtttttgtcc
     6001 gtctgcccat ccgttctgtt taatgagaag gggagtatga agagaataaa ggggcgagtg
     6061 ctcatcgccg aggacgacag cgccttcgcg gctcagttgg cctcggtgct cgagggggag
     6121 gattatcagg tcgtgctggc gcgcgacggc aacgaagcgg tcaggcagat cgccggcggg
     6181 gagctggata ttggcttcat cgatctcgcc atgcccggac tggacggcat gcaggtgctc
     6241 gaaaaagccg tcggcatcgc cccggacctg ccgctggtgg taattaccgg ccacgcctcg
     6301 atcgaccgcg ccatccaggc gacacggttg ggggcgttcg attttctcga aaagccggtc
     6361 aatctggagc gggtgctgct caccgtggag cgcgccctgg gttggcgtca gctggagcag
     6421 aagaacaact ggatggccgc cgagatcatg aaccgctacc gcatggtcgg caccagcccg
     6481 gggatgcagg atatctatgc gcgcatcgac caggtggccc ccaccgagag caccgttctg
     6541 atcaccggcg agaccggcac gggcaaggaa ctggtcgcca tggctatcca tctgcgcagc
     6601 catcgcgccg ccggaccctt tatgcgcgtc aactgcgccg ccattcccga caccctcatc
     6661 gagagcgaac tcttcggaca tcgcaaggga gccttcaccg gggcgaccga gaaccatgac
     6721 ggcaaattca tgcaggccgc ggggggcagc ctttttcttg acgagatcgg cgacctcagc
     6781 cagccggcgc aggccaagct gctgcgcgtc ctgcagtccc gcgaggtcga agcggtcggt
     6841 gccgcccggc cgcagcctgt cgacgtccgc atcatcgccg ccactaacaa gaacctcact
     6901 gaatgcatca gcaaggggct tttccgcgag gatctctatt atcgcgtctg cgtcatcgac
     6961 atcaatctcc ctccgcttcg cgagcgcaag gaggatatcc cggagctgag cggccatttt
     7021 ctgaactatt tctgcgggca gtacaaccgc cggctggagg gtttcacccc ggccgcactg
     7081 caaaccttga tgaactatca ctggccgggg aatgtccgcc agctgcgctc ctgcatcgaa
     7141 cgggcggtga tcctggctcg tggcgagagc ctctccgagg ccgacctgcg gttgatgatg
     7201 agcggcaagc cggtcaatga ggaggctccc gccacccttc gtcaggcccg ggagcgtttt
     7261 gaaaaaagct atatcgagca ggtgctgaaa agccacgatt ggaagatgga ggagagcgcg
     7321 gcgacgctag gcatcgaccg caccaatctc tataaaaaga tgcaaaagtt ggggatccat
     7381 cgggaatagc agggaaagat aaagcaggcc ggggggcagg cccgggacaa ttcggattaa
     7441 aaactaaaaa gcctccgtgt gctctgcgga ggctttttag ttttggcatc tgaggaggag
     7501 gagggaggtt ctgcggggca agaggagctg gacaggctgc cttgccccgg ccgctgccca
     7561 gccgggaagc ggctaaatgc tatgtcagtg acaggctcac tcagtcctgt acggctgctc
     7621 aggttgctgg atgcaacagg ctccaggccg ctacagatgc gatggcaagt cccactccaa
     7681 aggacgctca aagcgttgca gccactgtgt atacggcccg gataaacctg cggcgatgcc
     7741 tgtctcacga tgcttctgct tctgcgttag cctatacaac aagcatgcca aaaatgaaaa
     7801 gcggctgaaa aaagcggcgc cggctcagta ggggattttg gtcaggatct cccggatgag
     7861 cagaaaatcg gccaggggat ggggattcgg tgagggcgaa tcgagcgtct taacataaga
     7921 agaaacattg cgatatctga tagacgccga ggcatgggtc ttgcgcagaa gcttgaccag
     7981 gcccgggtga ctcttctcgg cctgacggag gaggcgcacc atgccgggga gtcccgccgg
     8041 atcgaaaccg gcttttacga ggtaggtggc gcatttccgg tcagcttcgc gttcaactgg
     8101 tttgggataa tccagaattg tatcctttgt gtacagatta aggaagagtt ggcgggcgat
     8161 ctcgggattc tggccgatga cactctgtgc ggccaccgcc cagccgtatt tggccgagag
     8221 cctttccacc ccgtcgcgcg ctgcgatgtg ggcgatctcg tgggcgataa cggcggccac
     8281 ctggtcggtg ctgccgaggg tttcgaacag accgcggtaa atgaagatct gtccgcccgg
     8341 gagtgaaaag tggttgaggt cgggttcgtt gacaagatgg acggagtagg tgaggccgct
     8401 ccagtgggag tgggggccga tttcggcggc cagctggttg agatactggc ccacctcctg
     8461 attgcggacg attttcagga gctgtccggc ctggactgaa acctcacggc cgaggacgat
     8521 ctcttcctcg atactgacaa aggcgagttt gccggtatcc cggctgccgg cacaggcgag
     8581 gagggccagg gcggcggcga ggaggaggct ggcggtaatc ttgattttca tgccttgatc
     8641 atctctttga attctgcttc ggtgaggacg gggatgccga gtgcccgggc cttgtccagt
     8701 ttgctgcccg cggcctcgcc ggcgatgaca taggaggtct tgctgctgac cgaggaggcc
     8761 ggccggccgc ccagggcgag gatcagtttt tccgcctcct cgcgggtgta ggaggagagg
     8821 gttccggtca ggacgagggt cttgccggcg aagaggtcgc ctctttgcgc ccgggagggg
     8881 gccatctcca cccccgcctg gcgcagccgt tcgataaccc ggccgttctc gggggtggtg
     8941 aaaaaatcgg tcacgctctc cgcgacctgg ggtccgatct catggacctg catcagctct
     9001 tcgctgtgcg cgctcatcag ccgctccatg gttccgaagg cctcaatcag gaggcgggcg
     9061 acgtgttcgc cgacatgacg gatgccgagg gcgaagagca gccgctcgag ggggcgggag
     9121 cggctggcct cgatggcggc gatgatgttg gctgccgatt tttcagccat acgttcaaga
     9181 ccggccagtt gtgcgcgggt gagggcatag agatcggcca catcccggac caggccgttc
     9241 tcgacgagct gatcgaccag cttttccccg agaccgtcga tatccatggc gcgtttggag
     9301 gcgaagtgga agatgcgttc cttgacctgg gccgggcagg agagattgat gcagcgccgg
     9361 gcggcctcat cctcgggccg caccgtatgg ctgccgcaga ccggacaggc ctcgggcagg
     9421 aggaaggggc gttcgtcgcc ggtgcggcgg ctctcaacga ccttgacgat ttcgggaatg
     9481 acatccccgg cgcgctgaac gacgacccag tccccgatgc ggatatcctt gcggtcgatc
     9541 tcgtcctggt tgtgcagggt ggcgcggctg acgcttacgc cgccgacctg gaccggctgc
     9601 aagatcgcaa ccggggtgag ggtgccggtg cggccgacct ggacctggat gttgagcacc
     9661 cgggtcacct cctgacgggc gggaaacttg aaggcgatcg cccagcgagg actcctgctc
     9721 ctggcgccga gttcctcctg ctggctgacg cggtcgacct tgacgacgat gccgtcgatc
     9781 tcatagggga gttgcccacg ccgttcgccc atggcacgat ggtaggccag gaccgcgccg
     9841 gcgccgcgga cgcgctcgat caggggattg acccgcagcc ccatgcttct gaagctctcg
     9901 agcatttcgg cgtgggtggt gaaggggagg gcttcggcct ggccgattcc gtagcagaag
     9961 atggccagag ggcggccggc ggtgatcagg gcgtcgagct ggcgcagcga ccccgccgcg
    10021 gcattgcgcg ggttggcgaa gagggactct ccccgttcct gccgttcgcg gttcagggtt
    10081 tcaaattcgc ggatgccgag aatgacctcg ccccgcacct cgagccggcc cgggggagcg
    10141 ttattgagct tgagggggat cgatttgatt gtgcggaggt tgcgggtgat ctcctccccg
    10201 gtataaccgt cgccgcgagt ggagccgagg gtgaagaggc catcgatgta gaccagttcg
    10261 acggcgaggc cgtccagttt gggctcgcaa acgtagtcga tctcctcctc gctgcccaga
    10321 gcccggcggg tgcgctgatc gaaggcgagt aactcctcct cgctgagggc attttccagg
    10381 ctgagcatgg gcacggtgtg gagggcgggg gggaaggctt ccaggggggc gcccccgatc
    10441 cgctgggtgg gggaatcagc cgttacgaac tccggaaagc gctgctcgag ctcgacgagc
    10501 tcacgcatga ggcgatcgaa ttcggcgtcc gaaatctccg gatcatcgag cccgtaatag
    10561 cgggtgttgt ggtagtgaat caggtcgcgc agctccctga tccgctcctc gggacggctg
    10621 ctcatggtta cctcaggccg aacatggcct tgacgatgta ccaggccatc atcggattct
    10681 ccttgagccg gcgcacgcag taggcatacc agttcttgcc gaaggggaca tagacgcgca
    10741 gcttgtgacc ggcctgcacg atgatatcac gcagctcctc gtcgacgccg agaagcatct
    10801 ggtattcata ctgcccggtg gtgagcttgt gcttgtggat gagttccagc cccttccagt
    10861 agagcctttc atcatgggtg gcgatgccga catagctgcc catgccgaag gcggtgtcga
    10921 cgaggaggcc gaagttgtcg ttgacggttt gtttatcctt gaaagccacc ttgcgcggtt
    10981 cgatatagat gcccttgcag atgcggtagt tggttttgtg ggcggagagg cgcacgacat
    11041 cagcgaggct gcggcgcagg taggcctgga tgacgatgcc ggtgcgcttg aattcgtcgt
    11101 tgatgcgcag gaagagttcg atggtctcgc tggtgacagc tgaatcctcc atgtcgatgc
    11161 ggacgaagat cccgagccga tccgcctt
//
LOCUS       DAOPWU010000088        10979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6033_length_10979_cov_4.608934, whole genome shotgun sequence.
ACCESSION   DAOPWU010000088 DAOPWU010000000
VERSION     DAOPWU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6033_length_10979_cov_4.608934"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /gene="pgk"
                     /locus_tag="PKM23_06325"
     CDS             <1..249
                     /gene="pgk"
                     /locus_tag="PKM23_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNY91125.1"
                     /translation="TIVWNGPMGVFEVAGFEKGTYAVAEAMANSGAVTVIGGGDSASA
                     VKKAGVAKKMSHVSTGGGASLELMEGKLLPGVAALTDK"
     gene            complement(319..1503)
                     /locus_tag="PKM23_06330"
     CDS             complement(319..1503)
                     /locus_tag="PKM23_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNY91126.1"
                     /translation="MKSTWWRGVAALLFLLLPVLHAQEVKNERGLAFAAVPSLSYSSG
                     EGWEYGGKLFFYQFGNGESRPYRWHLLLNGARSTEEKQDYYAFVDLPNLWGEGSRLDL
                     RVEYKDFGLDEFYGIGNLPSYRKELTDPDQPGFLDEKFYSYKHRWSAGYLNTQWPLRG
                     GRLRLLAGAAALRTSLSTYPLPNQLAQSPQPGMAGGWSNYARIGLVWDSRDQEAAPTS
                     GCWSDLLIEKSAAILGSDYDFARITATDRRYISLLPGLVYAQRLLVEHMPGHPPFYEM
                     AVISGSYQRIEGLGSGRSIRGIPRLLFVGPTKLLTNFELRLRLLDMRILRQELTFYGH
                     LFADGGKVLLKGEKASLSRYQWSQGAGLHVQWKKELIGALDVGRSQYNDMAVYLTFGN
                     LF"
     gene            2092..2487
                     /locus_tag="PKM23_06335"
     CDS             2092..2487
                     /locus_tag="PKM23_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91127.1"
                     /translation="MGQQQLLLLVLSVIIVGIAVVVGINMFSTSAESANVEAVTNDLM
                     HLASLAQQYYIKPTSMAGGGNSFASITMKNLIPSVTDGTYTNENGSYTCGTGTATQVI
                     LTGTPKRGTNVVTVTVTPTNVTTAIAAGE"
     gene            2670..4010
                     /locus_tag="PKM23_06340"
     CDS             2670..4010
                     /locus_tag="PKM23_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNY91128.1"
                     /translation="MAEFRYNGLNAQQKRIIGYIEAKNMREARKKTTALAGLHHFTLL
                     SIQEKATFIYRVRKLDGTILKGDQVAFSRDEVEVALRRLGFEDVRVQKIILDLRLKPP
                     FADVITFIRLSADLLREKLPFDEILGLLESDTSNKAMRKVIKEILVDLKDGKDGEEAF
                     GKQAYYLGRFPAKMLGVASKSGNMAEVFDNTARFLERDYEFRKSMRSALLMPAITTVV
                     LIASVIFYVGYIFPKTAELFLSFGSTLPPLTKNSLDLSHFLVRNIYWIIGLSLAIPLV
                     LYRLSVTPRGKIFVNKHMIKVPVIGSLLHKTSIEIFCRVFHSMYSGSGENLEVIRTAA
                     EACRNKYIEKQVKEVTIPMMVRDGASFVQALKQAKVFTENALSRLNAGAESGTLKRVT
                     LQIANYYERETTYRMKAIIDWIQVIIAFFIMLVMTLLTIVSSETALFQPQLPGM"
     gene            4014..5798
                     /locus_tag="PKM23_06345"
     CDS             4014..5798
                     /locus_tag="PKM23_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNY91129.1"
                     /translation="MVRKHQIASAIVEKLLQQDTRFTREKLQQAGKILENSSTPRTLA
                     DVLITDLGYDRHLVFRALCRIYAFREIRLSAASLTDGWIEFMQNFYKALDAKTREEFL
                     TLKLLPFGFSERGQNIRIFVCPDPTARTIESLMQRAGIKRYEVAYGRYEDVSEILARI
                     APQENEFLNLVKEVAAEVTIIDEEEENKVDEEELTSALNKSLLVNLFEGCLVDGVRKG
                     ASDIHIIPKDGNCTEIYIRLDGRLILWHRQDGIKPEAFLAVVKDRTRNVDRFEWEMAQ
                     DGFIQRRVDAFLIRFRVAILPIVSSALGRKYESVVIRILDDRKVITDLDKLGFDDYAR
                     SEFIKAIKKPQGMVILTGPTGSGKSTTLVAALSHVMTPELNILTVEDPVEYNIPGARQ
                     LKIGNKMNFDQAMRTILRHDPDIVMVGEIRDAKSAEIAIKLANTGHLTFTTLHTNDAS
                     SVVSRLYKMGIESFLIAYAINLVVAQRLMRTLCKHCKQPLPAAEAAIASSLGYTAEEI
                     SRATFYRAEGCPKCRNGYSGRMGIHEVLPFTPEIRRIILDASSDVDEDAIRTSAMKTG
                     MTTLRAAARRRAAEGLTTLEEVAAVTIE"
     gene            5815..6834
                     /locus_tag="PKM23_06350"
     CDS             5815..6834
                     /locus_tag="PKM23_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY91130.1"
                     /translation="MRDVLIIDYDPELREQVSHVLNGGVYALTFASDGVEALKLVKQK
                     RFDLILVDLLMPGALNGIQILQGMQRHAPASPLVVMSERNGHQELETAMRYGAREVLL
                     KPVHLELMKAVADRLTGGLVRPREGTVEPQLEAVKGRIDLAASDVTELESPAGAAADE
                     AGEARSQEPAAAVHCRAKLFTGLSDTTLEALMSRSRKVTLEAGQEIAFDACQTMVVVN
                     QGVARCWYKGLLISVLEPGDYIGETALFSRAGESLPLILDGNSGLEVCLLSRKLLRSF
                     YQEQGAHHLLHFAARVVQNLSDRLLHLYDELNHARRDGEPLSGVDEFHPLMEELSEES
                     DEWIC"
     gene            6818..8071
                     /locus_tag="PKM23_06355"
     CDS             6818..8071
                     /locus_tag="PKM23_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNY91131.1"
                     /translation="MNGFVDSAKVVSQDSSLEGIFKRILSQIPENMDLLEAQQTVAAA
                     VNALSAADRLALRERFESFLIHMREKEASDIEMGGVTANSHIWFRIQGKKMARVELGI
                     WPIGNTDIIAHSLLSEKQRERLFKRRAVDFSYTMQRPQGLWRWRATLYFDLGHLALNM
                     RAIAGQIYPYAQLGFHSAISRAFSLRYEKQGLILITGITGSGKSTTLDSIIDANNRSV
                     DGHIVIIGDPIEYIHNSRLCIIRHREVGRDVNSFKDGAVQALRQDPDIIVVGEMRDPD
                     TIMTCLEIVDTGHKVFSTLHTGCAVETIDRIIAECPGEEQERVRNRLADVLHVIISQK
                     LLPGINGRLIMAKEVLLVTTPVRAAIRNRNVQEIYQMISENNRQGMITLEQDLVRLVT
                     ERLVDEKVALDYANNKRRFQELLGN"
     gene            8084..9670
                     /locus_tag="PKM23_06360"
     CDS             8084..9670
                     /locus_tag="PKM23_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91132.1"
                     /translation="MRKKFSLGIFLDGQQLSCALLSRVGQRIQIELVENFHLEQPLLP
                     QERILSGVKLDDVRKKKGGTADDNPFELDVDFGTTAENVKPISEVSTNVDVLLRVLHT
                     MCPRESRLAFSLIDSCVLYKNIDGLPAAAPQKIKRAIWQDIFVEAPAEAKLENIGYLR
                     NGKTRYLAMVHDDPLVFSSTLFEAMRIMRSRPPQVALIDSLEFSLGHALFRRGLLPEA
                     EITAVVFFAQSFTKIIFFRGPLIEEVLPTIHEGSDSEQVCNTAYAKILFELDSGKISA
                     VNRLILTGDVDRTRAEAAFQNKFANVQATRFDFADLPLGHHAAKLTGRVAPWTAAIAL
                     AGKALEENVNTPYQQNYLPRRIRDRQSIYRVAWHGVALLAVLFLCFFFLTYKTVSGQQ
                     ELKHLRSSSTAMAMDLARLSLVAHEVDSLRAVISSFETGTALVDSLKSRSVRWSPVLR
                     QFSAAYSTAGPFFLQEIKGTSDGRIIAEAEVNRRSQVAELERLIIPSTVLSVASEGEK
                     SNSLLVTLECRGKSAGREGE"
     gene            9672..10616
                     /locus_tag="PKM23_06365"
     CDS             9672..10616
                     /locus_tag="PKM23_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91133.1"
                     /translation="MTYAVRNTLFIGAFWALIVAGGFFFVYGHQDDQLVIARRDNTLK
                     RQRLMDLLTLDRDRSALAEQLSGLEDFRQARDGTLAAKESPGETFDYLLRELSRNNSR
                     LEVNFTLKSQDSFLSLARRTYEIKGSGSFNDFYQLLCLLEEGPVFYDIHQMEMSAVGE
                     GVKGGGRGDVTFTLSFNGYNRAEGPAITTISASSIKPPQIADLVNGRVPAANRGSFVK
                     SWQGRGEVETPSVVKKEPPRNTEGLPEIDSSTKVLAIMPGAAVLKDHAGRTVRLRAGD
                     RVWGGTLETINAQQGKLSFSMQDENGGATRLVIPSGSN"
     gene            10627..>10979
                     /locus_tag="PKM23_06370"
     CDS             10627..>10979
                     /locus_tag="PKM23_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91134.1"
                     /translation="MRPFNAAVPLLLILVLASALCGQEGLEPEFTPPETLVSLSSSLD
                     IGTALDMLSEYAIRFAGKPIFDPTKQTGTINIEVAGMPWEKALQTILARRGLWYENRE
                     HFFQVAHPSSSGEGG"
BASE COUNT         2384 a         3164 c         3116 g         2315 t
ORIGIN      
        1 accatcgtct ggaacggacc catgggggtc tttgaggtgg ccggattcga aaagggcacc
       61 tacgcggttg ccgaggccat ggccaacagc ggcgcggtga cggtgatcgg cggcggtgat
      121 tccgccagcg ccgtcaaaaa agccggcgtg gccaagaaga tgagccacgt ctccaccggc
      181 ggcggggcca gcctcgagct gatggagggg aaactcctcc ccggtgtcgc ggcgctgact
      241 gacaaataat ccgaccgcac aagcgcaaaa ccgaaagcca ggcccctcag gagcctggct
      301 ttttttatgc ctatcaactc agaagaggtt gccgaaggtg agatagacgg ccatgtcgtt
      361 gtattgcgag cggccgacgt cgagggcgcc gatgagttcc ttcttccact gcacatggag
      421 gccggcgccc tgactccatt ggtagcggga gaggctggcc ttctcgccct tgagcagcac
      481 cttgcccccg tccgcgaaga ggtggccgta gaaggtgagc tcctggcgga ggatgcgcat
      541 gtcgaggagg cggaggcgca gttcgaaatt ggtgagcagc ttggtggggc cgacgaagag
      601 gagcctgggg atgccgcgta tgctgcggcc gctgccgagc ccctcgatgc gctggtaaga
      661 gccgctgatc accgccattt catagaaggg gggatggccg ggcatgtgct cgacaaggag
      721 acgctgggca tagaccaggc ccgggagaag gctgatgtag cggcggtcgg tcgcggtgat
      781 gcgggcaaaa tcatagtcgc tgccgaggat agcggcgctc ttttcgatga gcagatcgct
      841 ccagcagccg gaggtggggg cggcctcctg gtcgcggctg tcccagacca gtccgatgcg
      901 cgcatagttg ctccagccgc cggccatgcc gggctgaggg gactgggcga gctggttggg
      961 caggggatag gtcgataggc tggtgcgcaa ggctgcggcc ccggcgagca gccgcagtcg
     1021 gccgccgcga agaggccact gggtattgag atagccggcg ctccagcggt gcttgtagct
     1081 gtagaatttc tcgtcgagaa agcccggctg atcgggatcg gtcagttctt tacggtagga
     1141 ggggaggttg ccgataccgt agaactcgtc gaggccgaaa tccttgtact cgacgcgcaa
     1201 atcgaggcgg ctgccctcgc cccagaggtt ggggaggtcc acgaaggcat aatagtcctg
     1261 cttctcctcc gtggagcggg cgccgttaag gagaaggtgc cagcggtagg gccggctctc
     1321 cccgttaccg aactggtaga aaaagagctt gccgccatac tcccagcctt caccactgct
     1381 gtagctcaaa ctgggcacgg ccgcgaaggc gagcccgcgc tcgtttttaa cttcctgcgc
     1441 atgaagaaca ggcaaaagga ggaataaaag agccgcaaca ccccgccacc acgtcgattt
     1501 catattttct cccgaaccat tcaaagtaat gcaatcggtc tgtgcaatta ctgaacacaa
     1561 gttacgtaat taatagctct tataaaaatg aaaatatttc attgttcaat aaaataacac
     1621 ttgacattgt ggcagccgca aggtatattt aattgaatgg ctattaatcg ccataattga
     1681 ctcattttta aacggtgact attttggggt ggggtaatca caagagtgtg attgaggacg
     1741 tgtgcaaata gtccggcttc cttttttgtt ctgcaacctg gtctttttcc ctatcagttc
     1801 tgcactcact gcttcgctca cttcaatcca tctctgctac tgagataaca acggcgcatg
     1861 ccgcttgtat ttcggccggc tattattcta tggcacagga gttcctgcgg cccatggccg
     1921 cactatccat ttatgtggat acatggataa tagggcctta acctgacaga ctggaaggag
     1981 gtgtagctct cgccaataaa ttcatcttca ccgtaacacc ataactcacc gggtccggcg
     2041 agtgccggcc cgaatctgcc caccctttaa tagctgtgag gagacactat tatgggacag
     2101 caacaacttc tgcttctggt gctgagcgtg atcatcgtcg gcatcgccgt tgtagtcggt
     2161 atcaacatgt tcagcacgtc cgccgaatcc gccaatgtcg aggccgtaac caatgatctc
     2221 atgcacctgg cctccctggc acagcagtac tacatcaaac ccaccagcat ggccggaggt
     2281 ggcaattcct tcgcctcgat cacgatgaaa aatctgatcc catcggtcac cgatggtacc
     2341 tacaccaatg aaaacggatc ctacacctgt ggcaccggaa ctgctacgca ggtcattttg
     2401 accggaacgc ccaaacgcgg caccaatgtg gtcacggtca cagtcacacc gaccaatgtg
     2461 actacagcga ttgcagcagg cgaataaggt taactccgcc agatcggagc agggcatgaa
     2521 atcgtaatac tcatgccctc cggcggtgga atcggctggc ttccgttttt aaggggatca
     2581 gccgcagtaa atccgtaaat gcggggccct tggtgttcga gggccccgca gctccttttt
     2641 tcaatggtgc aggtgtgagg tgagggtgta tggccgaatt tcgctacaac ggtctgaatg
     2701 cgcagcagaa gcgcattatc ggctacatcg aagcgaaaaa catgcgggaa gccagaaaaa
     2761 agacgacagc cctggccggc ctgcaccatt ttacgctgct ctccattcag gagaaagcga
     2821 cctttatcta ccgtgtgcgc aaacttgatg gcaccattct caagggcgat caggttgctt
     2881 tttcacgcga tgaggtagaa gtggccctgc gccgcctcgg ttttgaggat gttcgcgttc
     2941 aaaagatcat cctcgatctg cgtttaaaac cgccctttgc cgatgtcatc accttcatcc
     3001 gtctctccgc cgatctgctg cgagaaaaac tgcccttcga tgaaatcctc ggcctgctcg
     3061 agagcgatac cagcaacaaa gcgatgcgca aggtcatcaa ggaaatcctt gtcgacctca
     3121 aggacgggaa ggatggggaa gaggcctttg gcaagcaggc ctattatctc gggcgttttc
     3181 ccgccaagat gctcggggtg gcctcgaaga gcggcaacat ggcggaggtc tttgacaata
     3241 ccgcccgctt cctcgaacgg gattatgaat tccgcaaaag catgcgcagc gccctgctca
     3301 tgcccgccat caccaccgtc gtcctgatcg cttcggtgat tttttatgtc ggctatatct
     3361 ttcccaagac cgccgagctc tttctcagct tcggatcgac gcttccaccc ctgaccaaga
     3421 acagtcttga tctgagccat tttctcgtac gcaacatcta ctggatcatc ggactctccc
     3481 tggccattcc cctggtactt tatcgtcttt ctgtcacgcc gaggggaaag atctttgtca
     3541 acaaacatat gatcaaggtg ccggtgatcg gctcgcttct gcacaagacc tcgatcgaga
     3601 ttttctgccg ggtcttccac agcatgtaca gcggctcggg tgagaacctc gaggtgatcc
     3661 gcacggccgc cgaggcttgc cgcaataagt atatcgagaa gcaggtcaag gaggtgacta
     3721 tcccgatgat ggtccgcgat ggcgccagct ttgtccaggc gttgaaacag gcgaaggtat
     3781 tcaccgagaa tgccctctcc cgtctcaatg ccggtgcgga atcgggcacg cttaaaagag
     3841 tgaccctgca aattgccaac tattatgaac gggagaccac ctaccgcatg aaagcgatca
     3901 ttgactggat tcaggtcatc atcgcctttt tcatcatgct ggtcatgacc ctcctgacca
     3961 tagtttcatc ggagactgca ctcttccagc cacagttacc ggggatgtaa ctcatggtac
     4021 gcaagcatca aatagccagc gccattgttg aaaagctgct gcagcaggac acccgtttca
     4081 cacgggagaa gctgcagcag gccgggaaga ttctcgaaaa ctcgtcgacg ccgcgcaccc
     4141 ttgcggatgt gctgattacg gatctcggtt atgaccgcca tctcgtcttc cgcgctctct
     4201 gccggatcta cgcctttcgc gaaatccgcc tcagtgcagc ctccctgacc gatggctgga
     4261 tagagtttat gcagaatttc tacaaggctc tggacgccaa gacccgcgag gagttcctca
     4321 ccctcaaact gctccccttc ggtttcagcg aacgcggaca gaatatccgt atttttgtat
     4381 gtcccgatcc cacagcgcgc accatcgaat ccctgatgca gcgggcgggc atcaaacgct
     4441 atgaagtcgc ctatggccgt tatgaggacg tcagcgagat cctcgcccgc atcgccccgc
     4501 aggagaacga gttcctcaat ctggtcaagg aggtggctgc cgaggtcacc atcatcgatg
     4561 aggaggagga gaacaaagtc gatgaggagg aactcacttc cgcgctcaat aaatccctgc
     4621 tggtcaatct cttcgagggt tgtctggtcg atggcgtgcg caaaggggcg agcgacattc
     4681 atatcatacc caaagatggc aactgcaccg agatttatat ccgtctggac ggacgcctca
     4741 tcctctggca ccggcaggat gggatcaagc ccgaagcctt tcttgcggtg gtcaaggacc
     4801 ggaccaggaa tgttgaccgt ttcgaatggg agatggccca ggatggcttt attcagcgcc
     4861 gtgtcgatgc ctttctaatc cgcttccgcg tggcgattct gccgatcgtc tcttccgccc
     4921 tggggcgcaa gtacgagagc gtggtcatcc gcatcctcga cgatcgcaag gtcatcaccg
     4981 acctggacaa actcggtttt gatgactatg ctcgctccga gttcatcaag gcgatcaaaa
     5041 agccgcaagg catggtcatc ctcaccggcc cgaccggaag cggcaagagc acaaccctgg
     5101 tggcggcgct ttcgcacgtt atgaccccgg agctcaacat cctcactgtc gaggatccgg
     5161 ttgagtacaa tatccctggc gcccgccagc tcaagatcgg caacaagatg aattttgacc
     5221 aggcgatgcg cacgatcctg cgccatgatc ctgatatcgt tatggtcggc gagatccgcg
     5281 atgccaaaag cgccgagatc gccatcaagc tggccaatac cggtcatctc accttcacga
     5341 ctttgcacac caacgacgcc agcagcgtgg tttcgcgtct ctacaagatg gggatcgaat
     5401 cctttctgat cgcctatgcg atcaatctgg tggtggcgca gcggctgatg cgcaccctgt
     5461 gcaaacactg caagcagcca ctgcctgcgg ccgaagccgc tatcgcctct tctctgggct
     5521 atactgccga ggagatcagc agggccacct tttaccgcgc agagggatgt cccaaatgcc
     5581 gcaatggata ttcgggacgg atgggcattc acgaagtact tcccttcacg ccggagatcc
     5641 gccgcattat tctcgatgcc tcttcggatg tggatgagga tgccatccgc acctcggcga
     5701 tgaagaccgg catgaccacc ctgcgtgcgg ccgcacgcag aagggccgct gaggggctga
     5761 ccactctcga agaagtggct gcggtaacca ttgaataagc ctgtgagata agccatgcga
     5821 gatgtactga ttatcgacta tgatccagag ctgcgcgagc aagtaagtca tgtgctcaac
     5881 ggcggggtct acgccctcac cttcgccagc gatggggtcg aggcgctcaa gctggtcaag
     5941 caaaagcgct tcgacctgat tctggtggat ctgctcatgc cgggggcgct caacggcatc
     6001 cagattcttc aggggatgca gcgtcatgcc ccggcctcgc ctctggtggt gatgagcgaa
     6061 cgcaacggtc atcaggaact ggaaaccgcc atgcgttatg gggcccggga ggttctgctc
     6121 aagccggttc atctcgagct gatgaaggcg gtggcagacc ggctgaccgg agggttggtg
     6181 cgtccccggg agggcacagt cgaaccccaa ctcgaggcgg tgaaggggcg gattgatctc
     6241 gcggcctcgg atgtaaccga gctcgaaagc ccagcaggag ctgcggctga cgaggctggg
     6301 gaggcccgaa gccaggaacc tgctgcagcc gttcattgcc gggcgaagct tttcacgggg
     6361 ctctctgata caaccctgga ggcgctgatg tccaggagcc ggaaggtgac actggaggct
     6421 ggacaggaga tcgctttcga cgcttgccag accatggtcg tagttaacca gggggtcgcg
     6481 cgctgctggt acaaggggct tctcatcagc gtactggaac cgggcgacta catcggcgag
     6541 accgcacttt tcagcagggc aggagagagt ctgccgctga tcctcgatgg caacagcggt
     6601 ctcgaggtgt gcctcctctc gcgcaagctg ctgcgcagtt tctatcagga gcagggggcg
     6661 catcatctgc tccattttgc ggcccgcgtc gtgcagaatc tgtcggatcg cctgctccat
     6721 ctttatgatg agctgaacca tgcccgccgc gacggcgagc cgctctccgg agttgatgaa
     6781 ttccacccac tgatggaaga attgtcggaa gaaagcgatg aatggatttg ttgacagcgc
     6841 aaaagtggtg agccaggata gcagcctcga aggcatcttc aagcgcatcc tctcccagat
     6901 ccccgaaaac atggacctgc tcgaggccca gcagaccgtc gcagctgccg tcaatgctct
     6961 ttcagccgcc gaccgcctgg cactgaggga gcgatttgaa tcctttctca tccatatgag
     7021 ggagaaggag gcctcggata ttgaaatggg cggggtcacg gccaattccc acatctggtt
     7081 tcgcattcag ggcaagaaga tggccagggt tgaactgggc atctggccga tcgggaacac
     7141 cgacatcatc gctcactccc tgctctcgga aaaacagcga gaacggctct tcaaacgccg
     7201 cgcggtcgat ttctcctaca ccatgcagcg gccgcagggg ttgtggcgct ggcgagccac
     7261 cctctatttc gacctcggcc accttgccct gaatatgcgc gccatcgccg gccagatcta
     7321 tccctatgcc cagctcggat ttcattctgc catctcgcgc gccttttcac tgcgctacga
     7381 aaaacaggga ctgattctga tcaccggcat caccggctcg ggcaagagca cgactctgga
     7441 cagtatcatc gacgccaata accgctcggt ggacggccac atcgtcatca tcggggatcc
     7501 catcgagtat attcacaatt cccgcctctg catcattcgc caccgcgagg tggggcgcga
     7561 cgtcaactct ttcaaggatg gggcggtgca agccctgcgt caggaccccg atatcatcgt
     7621 cgtcggcgaa atgcgtgatc cggataccat catgacctgc ctcgagatcg tcgacaccgg
     7681 tcacaaggtc ttttccaccc tgcacaccgg ctgtgctgtc gagaccatcg accgtatcat
     7741 cgccgagtgc cctggcgagg agcaggaacg ggtgcgcaac cgtcttgctg atgtgctgca
     7801 tgtgatcatc tcacaaaaat tgctgcctgg catcaacgga cggctcatca tggcaaaaga
     7861 ggtcctgctc gtcaccactc cggtgcgtgc agccatccgc aaccgcaatg tccaggagat
     7921 ctatcagatg attagcgaga acaaccgtca aggcatgatc accctcgaac aggatctggt
     7981 ccgccttgtc accgaacgtc tggtggacga aaaggtagca ctcgattacg ctaacaacaa
     8041 acgccgtttt caggaattat tgggcaatta gactatgaaa aacatgcgca agaaattttc
     8101 actcgggatt tttctcgacg gtcaacagct ctcgtgcgcc ctgctcagcc gggtgggaca
     8161 acgcatccag attgagctgg tcgagaactt tcatctggag cagccccttc ttcctcagga
     8221 acgcatattg agcggggtca agctcgacga tgtccgcaaa aaaaaggggg gaacggcaga
     8281 tgacaatccc tttgagctgg atgtcgactt cggcacaacc gcagaaaacg tcaaaccgat
     8341 ttcggaggtc tcgaccaatg tcgacgtcct gctgcgtgtg ctgcacacca tgtgcccgcg
     8401 cgaaagtcga ctggctttca gcctcatcga ctcctgcgtg ctctacaaga acatagatgg
     8461 cctgcctgcg gctgcgccgc aaaagatcaa acgcgccatc tggcaggata tctttgtcga
     8521 ggccccggcg gaggccaagc tggaaaacat cggctacctg cgcaatggca aaacccgtta
     8581 tctggccatg gttcacgatg atcccctggt attttcctcg actctcttcg aagcgatgcg
     8641 catcatgcgc tctcgtccgc cccaggtggc cctgatcgat tccctggaat tctcgctggg
     8701 acatgccctg ttccggcgcg gacttttgcc ggaggcggag atcactgcgg tggtcttttt
     8761 tgcccagagt tttaccaaga ttatcttctt ccgtgggcca ctcatcgagg aggtgctgcc
     8821 gactatccat gaagggtcgg attcggagca ggtctgcaac accgcttatg ccaagatcct
     8881 ctttgaactc gactcgggca agatttcggc tgtgaaccgg cttatcctga ccggagatgt
     8941 cgaccgcacg cgagcagaag ctgcttttca gaataaattt gcaaacgtcc aggcgacgcg
     9001 ctttgatttt gccgatttgc ccctcgggca tcatgccgcc aaactgaccg gacgggttgc
     9061 cccctggaca gcggccatcg ctcttgccgg caaggctttg gaggaaaatg tcaacacccc
     9121 ttatcaacag aactatttac cccggcgcat ccgcgatcgc cagtcgattt accgggttgc
     9181 ctggcacggc gtcgcccttc tggccgtgct tttcctctgt ttctttttcc tcacctacaa
     9241 gaccgtcagc ggtcagcagg aattgaaaca cctgcgttcc agttcgacgg cgatggccat
     9301 ggacctcgcc cgtctcagtc tggtcgcgca tgaggtcgac agcctgcgtg ccgtaatcag
     9361 ctccttcgag accggcaccg ccctggtcga ttctctcaag tctcgctccg tgcgctggag
     9421 cccggtgctg cggcagtttt ccgcggctta ttcgacagcg ggtccctttt ttctgcaaga
     9481 gatcaaggga acctccgacg gcagaatcat cgccgaagct gaggtcaacc gccgcagcca
     9541 ggtggccgaa ctcgaaagac tgatcattcc cagcactgtg ctcagtgtgg ccagcgaggg
     9601 agagaagtca aattcattac tggtcacact ggagtgccgg ggaaaatcag ccggaaggga
     9661 aggagagtga gatgacctat gcagtccgca ataccctgtt catcggagcc ttctgggcgc
     9721 tgatcgtagc cgggggattt ttcttcgtct atggtcatca ggatgatcag ctggtaatag
     9781 ccaggaggga taacaccctc aaacgtcagc gcctgatgga tctgctcacc ctggataggg
     9841 atcgctccgc gctggccgag cagctctccg ggctggagga ttttcgtcag gccagagacg
     9901 gcaccctggc cgccaaagag tctccgggcg agacttttga ctatttgttg cgggagctct
     9961 cccgcaacaa ttcgcgtcta gaggttaact ttactctcaa gagccaggat tccttcctct
    10021 ccctggcgcg cagaacctac gagatcaagg ggagcggttc cttcaacgat ttttatcaac
    10081 tcctctgcct gctcgaggag gggccggttt tctatgacat tcatcagatg gagatgtcgg
    10141 cggtgggaga gggggtcaag ggtggtgggc gaggtgatgt gacctttact ctttctttca
    10201 acggttataa ccgggctgaa ggaccggcca tcaccaccat ctcggcctca tccatcaagc
    10261 ccccacagat cgctgacctg gtcaatggac gcgtaccggc ggctaaccgg ggcagttttg
    10321 tgaaatcctg gcaggggaga ggtgaagtgg agacaccctc agtggtgaaa aaagagccac
    10381 ctcgcaatac cgagggactg ccagaaattg atagcagcac caaggtcctg gccatcatgc
    10441 ccggtgcagc tgttctcaag gaccacgccg gccggacggt gcgactgcgt gcaggggacc
    10501 gcgtctgggg tggcaccctt gagactatca atgcccagca gggcaaactt tctttttcca
    10561 tgcaagatga gaacggcggg gcgaccagac tggtcatccc ctccggctcg aactgaaagg
    10621 ccggatatga gaccctttaa tgcggctgtt ccgctcctcc tcatcctggt gctggcctca
    10681 gcgctgtgcg gccaggaggg actagagccc gaatttacac cccctgaaac cctggtgtcg
    10741 ctcagcagca gcctagatat cggcacggcc ctggacatgc tcagcgaata tgccatccgc
    10801 tttgccggca agccgatttt cgatccgacc aaacagaccg ggacgatcaa tatcgaagtc
    10861 gcgggcatgc cctgggagaa agccctgcag accatcctgg cccggcgcgg gttgtggtac
    10921 gaaaaccggg agcacttttt ccaggttgca cacccttcat cttcaggcga gggggggga
//
LOCUS       DAOPWU010000089        10978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6034_length_10978_cov_5.314566, whole genome shotgun sequence.
ACCESSION   DAOPWU010000089 DAOPWU010000000
VERSION     DAOPWU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10978
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6034_length_10978_cov_5.314566"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PKM23_06375"
     CDS             <1..959
                     /locus_tag="PKM23_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNY91135.1"
                     /translation="LTIWLVTRESVRMQRVEISEAALGEEIGLLRRSLSRQLTITESA
                     RRLYDLLIKPFAEEIGRSEHLIILPHGALHYLPFALLQDEQKRYLGLEHTLSVAASAS
                     VLSHCLAKGEHYAGRERRQMQVLAFGNPDLGDIQYDLAFAEREVKSLKRYYPVVRAFT
                     GVDASENRLLAEIPSPPLLLFSCHGVFDEANPMLSALLLAPGGGEDGRLEAHEIFGLK
                     MEAFVVAMSACETGVGALRSGDEVIGLSRAFTYAGAAAQLSSLWKVDDLATAVLMKRF
                     FRYLADGDARPEALRKAQRLVMQEINPYPAFWAAFQISGDYR"
     gene            1134..2408
                     /locus_tag="PKM23_06380"
     CDS             1134..2408
                     /locus_tag="PKM23_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNY91136.1"
                     /translation="MKTTIALILAGGRVDELSVLTLYRPKSALPFGGLFRVIDFPLSN
                     LLHSGIERVGILSQYRSDSLINHIGSGAAWDMIGSQRGITMLPPMKGTQSSDWYKGTA
                     DAVYQNRDYILRHDPDYILVLSGDHVYSMDYQKMIAFHERMEADITCAFVEVKSDGME
                     RFGQGLIAGDDPEGGLLTRYVEKPSESISPWASMTVYLFNRDTLRYIMDEMMRPPATE
                     HFGRDILPVLIDKKRICGYKFRGPWAYTRTIDEYYSASMDLLSEKPLLGLDEWPVRTN
                     LDNERIRDRAPALIGPGAQCSQALVHHGCEIQGQVHNSILFPGVRVKAGATVENSILM
                     FDTVVEQGAQLNRVITDVEVAIGANAVVGTGEATGVNKEYPNLLKSGITIIGRNTRLP
                     GGIRVGRNCIIYPNKKEAEFTSLTIANGRTIK"
     gene            2405..3661
                     /locus_tag="PKM23_06385"
     CDS             2405..3661
                     /locus_tag="PKM23_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNY91137.1"
                     /translation="MRNTVAFLLAGGMGSRLNILGWMRAKPAVPFGGCYRLIDFTLSN
                     AMYSGVQQIGILTQYRPYSLMGHIGSGESWDLVGRSRCAKILPPSTGIDDYDWYRGTA
                     DAVAQNMQFVHHYNAERLLVLSGDHIYRMNYAPMVEFHKEKKAHITIAMMRVPWEETR
                     HFGIGITNKEQRIVDWEEKPEKAKSNLASMGVYVFDVAYLEEALAGRTGHDFGKNIIR
                     DAINAREVYAYPFEGYWADVGTIKAYWQASMEILQPESGLRLQEWGVRTNFDEEGLKG
                     DRPPSRIGSRARVVNSLISAGCHIEGVVQNSILSPGVRVEKGAEVIDSVIMQDSCIGA
                     GARLFRVIADKNARFGAGAIVGGEEADEPNEKYPEHLYSGLTVIGKRAAIPDGLRIAA
                     NTIVEPHAGESSFASITLGVGSYVKR"
     gene            complement(3711..4484)
                     /locus_tag="PKM23_06390"
     CDS             complement(3711..4484)
                     /locus_tag="PKM23_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNY91138.1"
                     /translation="MKYRILMIALLIASSLPFSAARAQINTERFRRDARQKGLEGNAD
                     IDLSIMTGNTDFRLIGLSSRLNYSWSRAYTFLVLNGGIGWNAGQRFMDQNTAHLRHVL
                     ILSPRLQNEWFVQFDSNRKRNLKERELIGVGLRTKLLTTSAVKLRLGAAYMYERERYE
                     LAEGNSHPHSLATHRLSSYLTLETGKGGGVSLLNVTYFQPALTDWGDSRLLSENAAAV
                     DLAANLALKVSITLRYDSRPPDGVKKLDTVSKSSLSVNF"
     gene            4872..5177
                     /locus_tag="PKM23_06395"
     CDS             4872..5177
                     /locus_tag="PKM23_06395"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNY91139.1"
                     /translation="MEDQGSLFKCQTRQEFVVELEANPSTGFAWQISKSDPDHVRLLR
                     EETLPADRKRLGAPGKQLFYFQAMAIGRAPLKLEYRRPWEKDAEPAKTFSVTLVIHK"
     gene            5228..6172
                     /locus_tag="PKM23_06400"
     CDS             5228..6172
                     /locus_tag="PKM23_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNY91140.1"
                     /translation="MRLCRWLFCAAVLLSCTPLYSQVLDESQAQPAVRQQLTKLRAEI
                     TKQKLSFQVGYNTAMTRTIDQLCGSKPPKNWYNTAKMKNLTKLAPATLKSSLALPARW
                     DWREHNGVTAVRDQGNCGSCWAFGTIGSFESFLLVKQSISSDLSEQHLVSCNGEGYGC
                     GGGWWVFDMLINPGAVQESLFPYAASDLPCPSGLTYAYQASGWAYVDGENQVANTQKI
                     KEAIYQLGPVAAAVYVGSYFQAYTGGVFDKEEAESGGFLCCASSHNVNHAILLVGWDD
                     ADGVWILRNSWGTGWGEGGYMRIKYGVNKVGFAAASVY"
     gene            complement(6381..7049)
                     /locus_tag="PKM23_06405"
     CDS             complement(6381..7049)
                     /locus_tag="PKM23_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNY91141.1"
                     /translation="MFKSDTLSTTKGDIVLTFLGHGTLMLQFGKTVVHVDPVSEYSDY
                     SALPKADLILVTHAHSDHLDPEAIALIRQDSTRIVAAPVCRTKLPEATLLANGDKIQI
                     GEVAVEAVPAYNLIHMRASKQPFHPRGEGNGYIIRFGGMSVYIAGDTENTPEMKALKG
                     IDVAFLPMNLPYTMTPEMVADAARAFQPRILYPYHYGDTDTGKLIDLLKGEKKIEVRV
                     RDLH"
     gene            complement(7244..8116)
                     /locus_tag="PKM23_06410"
     CDS             complement(7244..8116)
                     /locus_tag="PKM23_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNY91142.1"
                     /translation="MKSCWTIFLLLLPFPAAGQNVVINEIGWMGTIASTSDEWIELYN
                     PTPHAIDLQGWSLKAADGIPAIRLTGVIPAQGYYVLERTDDQTISDRPAEQLFTGDLK
                     NSGEWLILRSSDSLLVDQVRCDSSGWFAGTNNPKRSMERRHPLLSGMLPESWADNDTL
                     TRNGLDAAGHPLSGTPGAPNSVYDHTLPVGQSSRRLPEAEELLWAWPNPFNPGIMLAW
                     GARGEEETVEILDLLGRLVRSWPSPPAARGGRAQLFWDGCDAAGRPVATGTYICRLLR
                     GRTIAAQLRLTRCR"
     gene            8270..9064
                     /gene="rsmA"
                     /locus_tag="PKM23_06415"
     CDS             8270..9064
                     /gene="rsmA"
                     /locus_tag="PKM23_06415"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNY91143.1"
                     /translation="MSYPIRPKKSLGQNFLVDGNVLHNIAEALDLAPGDTVLEIGPGT
                     GVLTELLQPRVARLIAVEIDRNLAALLRSRFAGVDNFTLVEGDILEMEPMLLAGEQPL
                     RVVGNIPYYITSQIIFHFFDRRRNLVDMALLMQREVAERIAAGPGDPDYGILAVMSRT
                     FAEAEILLRVPRTVFKPQPGVDSALVRWRFHDRHTRTLADEGLYRRLVRTAFGQRRKM
                     MRKSLRDRFNLEALAAFDLTRRPEELDVADWLAMANALAAAAPARP"
     gene            9127..>9401
                     /gene="recO"
                     /locus_tag="PKM23_06420"
     CDS             9127..>9401
                     /gene="recO"
                     /locus_tag="PKM23_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNY91144.1"
                     /translation="MPLSKTEAIVLHTRKQGETSKILSLYTLHYGRLSVMAKGARSLK
                     SRYSGVLEPLTHIQIVFYRYEARDLHYLSQAEIVTPFASIHGQLGKMA"
     assembly_gap    9402..9501
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9502..9813
                     /locus_tag="PKM23_06425"
     CDS             <9502..9813
                     /locus_tag="PKM23_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HNY91145.1"
                     /translation="SGGYRCAGCEGRSDEGEPLSRQAMQALHFLGHSELEQSVTLRVN
                     AELGRELDQFLLANLRWHLEGAHYLKSIQYLEKLQQSIHKTQEEEQGKEYGQPDPHHP
                     G"
     gene            9782..>10978
                     /locus_tag="PKM23_06430"
     CDS             9782..>10978
                     /locus_tag="PKM23_06430"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNY91146.1"
                     /translation="MANPTPTTLDKIISLCRRRGFIFQSSEIYGGLNACYDYGPLGVE
                     LKRNVKDFWWNWMVRRRRDVVGLDAAILMAPRVWEASGHVAGFTDPMVDCKVCKRRFR
                     ADMVPEPGKCPECGGQMTDIRQFNLMFKTFMGAVEDSASVVYLRPETAQGIYVNYRNV
                     LDSSRQKVPFGIGQIGKAFRNEITTENFIFRTREFEQMEMQFFVKPGTDMEWFESWKE
                     DRKNYYSALGINPAKTRFHQHGEDELAHYAAAAFDIQYEFPFGWKELEGIHNRTDFDL
                     KRHQEYAGVDLSYFDEVTRERYVPYIIETSAGVDRTMLCILCDAFEEQELEKDTRTVL
                     HFHPAIAPVKAGIFPLVKKEGMPEIAHQIEAMLQPYYAVFYDESGAVGRRYRRQDEVG
                     TPFCITV"
BASE COUNT         2082 a         3257 c         3358 g         2181 t
ORIGIN      
        1 gtctcacgat ctggctggtc acccgggaat ccgtccggat gcagcgggtt gagatcagcg
       61 aggctgcgct cggggaggag atcggcctgc tccgccgcag tctctcccgg cagctcacca
      121 ttaccgagag cgcgcgccgt ctctacgatc ttttaatcaa gcccttcgcc gaggagatcg
      181 gccgcagcga acatctcatc attcttcccc atggcgcgct gcattacttg ccatttgccc
      241 tgctgcaaga cgagcaaaag cgctatctcg gcctggaaca caccctctcg gttgcggcga
      301 gcgcttccgt tctcagccac tgcctggcca agggggagca ttacgccggg cgggagaggc
      361 gccagatgca agtgctggca ttcggcaatc ccgacctggg cgacatccag tacgatctcg
      421 cctttgccga gcgggaggtg aagagcctga agcgctacta tcctgtggtg cgtgcgttta
      481 cgggtgtgga cgccagtgag aaccggctgc tggccgagat cccctcgccg cctctgcttc
      541 ttttcagctg tcatggagtc tttgacgagg ccaaccccat gctttcggca ttgctgctgg
      601 cgcccggggg cggggaggac ggccgcctcg aggcccacga aatattcggc ctcaaaatgg
      661 aggcctttgt ggtggcgatg agcgcctgcg agaccggggt cggggcgctg cgcagtgggg
      721 acgaggtgat cgggctcagc cgtgcattta cctacgctgg cgcggccgcg cagttgtcga
      781 gcctgtggaa ggtcgacgat ctggccaccg ccgtgctgat gaagcgtttc ttccgttatc
      841 ttgctgatgg tgatgcgcgc cccgaggccc tgcgcaaggc ccagcggctg gtcatgcaag
      901 agatcaatcc ctatccagcc ttctgggcgg ccttccagat cagcggggac tatcgctgac
      961 ctttctcccg ttggtgcata gaggcccgcc ggccggccga gaaaatcttg ttgcgttgtt
     1021 cttaaaaact tgatatttta gcccggaagc cctgcgcaag cccctgctgc tgtggaatgg
     1081 tcttccaggt gataaacatc ctgcggacgc agaaccctta ccggtggagg cccatgaaaa
     1141 caaccatcgc tttgatcctg gccggcggcc gggtcgacga actttctgtg ctcacgctct
     1201 accgacccaa atcggccctg ccgttcggtg ggctttttcg cgtcatcgat tttcccctca
     1261 gcaatctgct ccattccggc atcgaacgtg tcgggatcct ctcgcagtac cgttcagact
     1321 ccctcatcaa tcacatcggt tcaggcgcag cctgggatat gatcggcagc cagcgcggca
     1381 tcaccatgct gccgccgatg aaggggacgc agagttcgga ctggtacaag gggacagccg
     1441 atgctgtata tcagaaccgc gattacattt tgcgccatga tcccgattat atcctggtcc
     1501 tttccggcga ccatgtttac agcatggact atcagaagat gatcgccttt cacgaacgca
     1561 tggaggccga catcacctgc gcctttgtcg aggtcaagag cgacggcatg gagcgcttcg
     1621 gccaggggct gattgccggg gatgatccgg aaggcgggct gctgacgcgc tatgtggaaa
     1681 agccgagcga atcgatcagc ccctgggcct cgatgacggt ctatctcttc aaccgcgaca
     1741 ccctgcgcta tatcatggac gagatgatgc gtccccccgc aaccgagcac tttgggcgcg
     1801 acattctgcc ggttctgatc gacaagaaac gaatctgcgg ctacaagttt cgcggaccct
     1861 gggcctatac ccgcaccatc gatgaatact attcggcgag tatggacctg ctctccgaaa
     1921 agccgctcct gggcctcgat gagtggccgg tccgcaccaa cctcgacaat gaacgcatcc
     1981 gcgaccgcgc gcccgctctg atcgggcccg gcgcccagtg cagccaggcc ctggtccatc
     2041 atggctgcga gatccagggt caggtgcaca attcaatcct ttttccgggg gtgcgcgtca
     2101 aggcaggggc gacggtggaa aattcgatcc tgatgttcga tacggtggtc gagcagggcg
     2161 cccagctgaa ccgcgtcatc accgacgtgg aggtcgccat cggcgccaat gcggtcgtcg
     2221 gcaccggcga ggctacgggc gtcaacaagg agtatcccaa cctgctcaag tcgggaatca
     2281 ccatcatcgg ccgcaatacg cggctccccg gaggcatacg cgtggggcga aactgcatca
     2341 tctatcccaa caaaaaggaa gccgaattca cgagcctgac tattgccaat ggaaggacga
     2401 tcaaatgaga aataccgtcg cgttcttgct cgcgggcggc atgggcagcc gcctcaatat
     2461 cctcggctgg atgcgcgcca aaccggctgt tccgttcggc ggttgttacc ggctcattga
     2521 ttttaccctc agcaatgcca tgtactcggg ggtccagcag atcggcatcc tcacccagta
     2581 tcgtccctat tccctgatgg gccatatcgg ctcgggggag tcctgggatc tggtcgggcg
     2641 cagccgctgc gccaagatcc tccccccctc cacgggcatc gacgactatg actggtatcg
     2701 cggcaccgct gatgctgtgg cccagaacat gcagttcgtc caccattaca acgcggagcg
     2761 cctcctcgta ctctccggtg atcatatcta tcgcatgaat tatgccccca tggtcgagtt
     2821 ccacaaggag aaaaaggccc atatcaccat cgcgatgatg cgcgtgccct gggaggaaac
     2881 ccgccatttc ggcatcggca tcaccaacaa ggaacagcgc atcgtcgact gggaggagaa
     2941 acctgaaaag gcgaagagca atctcgcctc gatgggggtg tacgttttcg acgtcgctta
     3001 tctggaagag gccctggccg gccgcaccgg acacgatttc ggcaagaaca ttatccgcga
     3061 tgcgatcaac gccagggagg tctatgccta tccctttgag ggctactggg cggatgtggg
     3121 gacgatcaag gcctactggc aggcgagcat ggagatcctc cagcccgagt cgggactgag
     3181 gctgcaggag tggggggtgc gcaccaactt tgacgaggag ggcctcaagg gcgaccggcc
     3241 gccctcccgc atcggcagcc gggcgcgcgt ggtcaattcg ctgatttcgg caggatgcca
     3301 catcgagggc gtcgtgcaga acagcatcct ctccccggga gtcagggttg aaaagggggc
     3361 agaggtgatc gattcggtca tcatgcagga cagctgcatc ggggccgggg cgcgcctctt
     3421 ccgggtcatc gccgacaaga acgcccgctt cggagccggc gccattgtcg gcggcgagga
     3481 ggccgacgaa ccgaacgaaa aatatccgga acatctctac agcgggctga ccgtgatcgg
     3541 caagagagcg gccattcccg atggactcag gatcgccgcc aacaccatcg tcgagcccca
     3601 tgccggagaa agcagttttg ccagcatcac cctcggcgtg ggttcctacg taaaacggtg
     3661 atcgactgcc atataccgtg cgcagacgcc cctgaagagg ggcgtttttt ttaaaaattg
     3721 accgagaggc tgctcttgct gacggtatcc agctttttga cgccgtcagg cggccggctg
     3781 tcgtagcgga gggtgatgct gactttgagc gccagattgg cggcgaggtc aacggcagcg
     3841 gcgttttcag agaggagccg gctgtcgccc caatctgtca gggcgggctg gaaataggtg
     3901 acattgagca gggagacccc gccgcccttg ccggtctcca gggtcagata gctgctcagg
     3961 cggtgggtgg cgagtgaatg gggatggctg ttgccctccg ccagttcgta gcgttcgcgt
     4021 tcatacatgt aggccgcccc gaggcggagc ttgaccgcgg aagtggtcaa cagtttggtc
     4081 ctcaacccca ccccgatcag ttcccgctcc ttcaggtttc gcttgcggtt gctgtcgaac
     4141 tggacgaacc actcattttg cagccggggg ctcaggatga ggacgtggcg cagatgagcg
     4201 gtattctgat ccatgaagcg ttggccggcg ttccagccga tgccgccgtt gaggaccaga
     4261 aaggtatagg cgcgggacca ggagtagttg agccggctgc tgaggccgat gagccggaaa
     4321 tcggtgtttc cggtcatgat tgagaggtcg atgtcggcat tgccctccag ccccttttgc
     4381 cgggcgtccc gacggaagcg ttccgtgttg atctgggccc gggcggcgct gaagggcagg
     4441 gaggaggcga taaggagtgc aatcatcagg attctgtatt tcatggcttt ctcgcgttga
     4501 ggggcggttg ttttcagggg agcaatttac cactttgcct gcataaaatc aacccctgat
     4561 cccgccagat cccccctccc ctaaatcttt ctctcacctg gcaccttgcg ccgcctgtcc
     4621 ccccgaaatc tggttcccac ctggtacctt tagccttgat cctgctcact tttcggctat
     4681 aaccttcttt agcgcatcaa tttcacttgc atttcatccc aaattatgtt acactcattt
     4741 taggggccag gccgccccgg agattcatgt tgtaactcgg gagggtagag atggcccatt
     4801 tcgagacctt gttttgcgca tgcctgatcg tgttagcctg ttcacagaat tcgccctcta
     4861 acctgctcac catggaagat caaggctctc tcttcaaatg ccagacccgt caggaattcg
     4921 tcgtcgagct tgaagccaat ccctcgacgg gatttgcctg gcagatcagc aagagcgatc
     4981 cggatcatgt gcgcctgctg cgcgaggaga cccttccggc tgaccggaag cgtctcggcg
     5041 ctccgggcaa acagctgttt tacttccagg cgatggccat cggccgcgca cccctgaaac
     5101 tggaataccg gcgcccctgg gaaaaggacg ccgaacccgc caagactttt tccgtcaccc
     5161 tggtcatcca caagtaatcg gcatctgtgc agcatccaac catagaacag ctaaaaggga
     5221 ggtggctatg aggttatgtc gttggctttt ctgtgctgcc gtgcttttgt cctgcacgcc
     5281 gctttattcc caggtgctcg atgagagtca ggcccagccc gccgtccgtc aacagctcac
     5341 caaactgcgg gcagagatca ccaaacagaa actctccttt caggtcggct acaataccgc
     5401 gatgacccgg acgatcgatc agctctgcgg gagcaaaccg ccgaagaact ggtacaacac
     5461 cgccaagatg aaaaatctga ccaaactcgc cccggccact ctcaagtcaa gcctcgccct
     5521 gccggccaga tgggactggc gcgagcataa cggggttacg gcagtgcgcg accaggggaa
     5581 ctgcggcagc tgctgggcct tcgggactat cggcagcttc gagtcctttt tgctcgtcaa
     5641 gcagagcatc agcagcgatc tttcggagca gcatctggtc tcctgtaatg gagagggcta
     5701 cggctgcgga ggagggtggt gggtctttga catgcttatc aatccgggcg ccgtccagga
     5761 atcccttttt ccctatgccg cctccgatct cccctgccct tccgggttga cctatgccta
     5821 tcaggccagc ggctgggcct atgtggacgg tgagaatcag gtcgccaaca cccagaaaat
     5881 caaggaggcg atctatcagc tcggaccggt cgccgccgcc gtctatgtgg ggagctattt
     5941 ccaggcctat accgggggcg tttttgacaa ggaggaggcg gaatcgggcg gattcctctg
     6001 ctgcgcctct tcccacaatg tcaaccacgc catcctgctg gtgggctggg atgacgccga
     6061 cggggtctgg atcctgcgca acagctgggg cacgggctgg ggtgagggcg gctacatgcg
     6121 gatcaagtac ggcgtcaaca aggtggggtt cgcagcggcc agcgtctatt gagcgccggc
     6181 ctgtccgggg cggcagccct gtgcaggcct ggcagaagga cgtgcactgg cttctggcca
     6241 gggcagcgcc acagtgaaga ctcgggcgtt tttgcaaaac aggcgtgcaa tgggcaccgg
     6301 cgcctggtcg tgcctgtgcg ctcctgcaga aaagcaatgg aatgggctct gatgagccca
     6361 ttcttttttg taccgtactg ctaatgcaga tcccgaaccc gcacctcgat ctttttctct
     6421 cccttgagga gatcgatgag cttaccggtg tccgtgtctc catagtggta gggatagagg
     6481 atgcgcggct gaaaggccct ggccgcatcc gccaccatct cgggggtcat ggtgtagggg
     6541 aggttcatcg ggagaaaggc gacgtcgatc cccttgaggg ccttcatttc gggcgtattc
     6601 tcggtgtcgc ccgctatata gacgctcatc ccgccgaagc ggatgatgta gccgttgccc
     6661 tcgcccctgg gatggaaggg ctgcttgctg gcgcgcatgt ggatgaggtt gtaggctggc
     6721 accgcctcga cggccacctc gccgatctgg attttgtcgc cattggcgag cagagtcgcc
     6781 tcaggaagct tggtcctgca gaccggcgcg gcgacgatgc gggtggagtc ctggcggatc
     6841 agggcgatgg cttccggatc gaggtggtcg gagtgggcgt gggtgaccag gatcaggtcc
     6901 gccttgggca gtgcgctgta atcgctgtac tccgagaccg gatcgacatg gacgacggtt
     6961 ttgccgaact gcagcatcag ggtgccgtgc ccgaggaagg tgaggacgat atcgcccttg
     7021 gtggtggaga gggtatcact cttgaacatg gttgtgctcc atgctggagt taataggagg
     7081 atgatagaca gggtaatgat atattttttc ataggctcct cgctctgttg ggtgtcagag
     7141 ggcgcgagct gcgcccgggg cttccctctt aaatctaggg ctcccggcgg caaaaatcaa
     7201 ccctaaagtt ggcgaaagcc ggaggcaggg agggattgcg ccttcagcgg cagcgggtga
     7261 ggcggagctg ggcggcgatg gtgcggccgc ggaggaggcg gcagatatag gtgccggtgg
     7321 ctaccggccg gccggcggcg tcgcagccat cccagaagag ctgcgcccgg ccgccgcgcg
     7381 cagccggggg ggagggccag gagcgcacga ggcgcccgag caggtcgagg atttcgacgg
     7441 tttcctcctc gccgcgggcg ccccaggcga gcatgatgcc gggattgaag gggttgggcc
     7501 aggcccagag gagttcctct gcttccggca ggcgtcggct gctctggccg accgggaggg
     7561 tgtggtcgta gacgctgttg ggcgcgccgg gggtgccgga gaggggatgg ccggcggcat
     7621 cgagtccgtt gcgtgtcagg gtgtcattgt cggcccagct ctccgggagc atgccgctga
     7681 ggagggggtg cctccgttcc atactgcgct tgggattgtt ggtgccggcg aaccagccgc
     7741 tcgagtcgca gcgcacctgg tcgacgagca gggagtcgct gctgcgcagg atgagccatt
     7801 cgccgctgtt cttgaggtcg ccggtgaaga gctgctccgc cggccggtcc gagatggtct
     7861 ggtcgtcggt gcgctccagc acataatagc cctgcgccgg gatgacgccg gtgagtcgga
     7921 tggccgggat tccgtcggcg gccttgagcg accaaccctg cagatcgata gcatgcggcg
     7981 tgggattgta gagctcgatc cattcgtcgc tggtgctggc tatggtcccc atccagccga
     8041 tctcgttgat gacgacgttt tgccccgcgg ccggaaaagg caggaggagc agaaatattg
     8101 tccagcagct tttcatactt taatctcatc atttctgagc aaaaagtcaa gggacgaaat
     8161 cgccactgcc ggggggaggt cgggcaaagc ctcctttaaa aaactgtggc ttttttgctg
     8221 attttcccct aacttgtaga caattcacaa ataaattcct gcgagtgaga tgagctatcc
     8281 catccggccc aaaaagagcc tgggccagaa ttttctggtc gatggcaacg tgctgcataa
     8341 catcgccgag gccctcgatc tcgcacccgg ggatacggtg ctggagatcg gtccgggcac
     8401 aggagtgctg accgagttgc tgcagccgcg cgtggcccgg ctgatcgcgg tcgagatcga
     8461 ccgtaatctg gccgccctgc tcaggagccg attcgccggg gtggacaatt tcaccctggt
     8521 ggagggggac atcctcgaga tggagccgat gctcctggcc ggggagcaac ccctgcgtgt
     8581 ggttggcaat atcccctatt atatcacctc gcagatcatc tttcatttct tcgaccgccg
     8641 ccgcaatctg gtcgatatgg ccctgctgat gcagcgcgag gtggcggaac ggatagccgc
     8701 cgggccgggc gaccccgatt acggcatcct ggcggtgatg agccggacct ttgcggaggc
     8761 ggaaatcctg ctgcgtgtgc cgcgaacggt tttcaagcct cagcccgggg tcgattccgc
     8821 cctggtgcgc tggcgttttc atgaccgcca tacccgcacc ctggccgatg aggggctcta
     8881 ccgccgcctg gtgcggaccg ctttcgggca gcggcgcaag atgatgcgca aatccctgcg
     8941 cgacagattc aatctcgaag ccctggcggc tttcgacctc acccgccgcc cggaagagct
     9001 ggatgtggct gactggctgg caatggccaa cgccctcgcg gccgccgcgc ctgcccggcc
     9061 ctagctgctc tgcagagcct cccggaacgc atcggccgtt atgttcgatt tgctggaaag
     9121 ggtgtcatgc ccctgagcaa aaccgaggcg atcgtgctgc acacccgcaa acagggtgag
     9181 accagcaaga tcctgtcgct ctacaccctc cattatgggc gtctgagcgt gatggccaaa
     9241 ggggcgcgca gcctcaagtc ccgctacagc ggtgtgctcg agccgctcac ccatatccag
     9301 atcgtctttt accgctatga agcgcgcgat ctgcactatc tgagccaggc ggaaatcgtc
     9361 acccccttcg cctcgattca tggacagctg ggcaagatgg cnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nagcggcggt taccgctgcg ccggctgcga ggggaggagc
     9541 gatgagggcg agcccctctc ccgccaggct atgcaggcgc tccactttct cggccactct
     9601 gaactcgagc aaagtgtcac cctccgcgtc aatgcggagc tggggcgtga actggaccag
     9661 ttcctgctcg ccaatctgcg ctggcatctg gagggcgccc attatctcaa gagcatccag
     9721 tatctggaaa aattgcagca aagcatccat aaaacccaag aggaagaaca aggaaaagaa
     9781 tatggccaac ccgaccccca ccaccctgga taagatcatc tcgttgtgcc gccgccgcgg
     9841 cttcattttt cagtcgagcg agatctatgg cggattgaac gcctgctacg attacggccc
     9901 cctcggcgtc gagctcaaac gcaatgtcaa ggatttctgg tggaactgga tggtgcgccg
     9961 ccgccgcgat gtcgtcggcc tcgacgccgc catcctcatg gcccccagag tttgggaggc
    10021 ctccggccat gtcgccggtt tcaccgaccc catggtcgac tgcaaggtct gcaaacgccg
    10081 ctttcgcgcc gacatggtcc cggagccggg caagtgcccc gaatgcggcg gccagatgac
    10141 cgacatccgt cagttcaacc tgatgttcaa gacctttatg ggggcggttg aggacagcgc
    10201 cagcgtcgtc tatctccgtc ccgagaccgc tcagggcatc tacgtcaact atcggaatgt
    10261 cctcgactcc agccgtcaga aagtgccctt tggcatcggc cagatcggca aggccttccg
    10321 caacgagatc acgaccgaga attttatctt ccgcacccgc gagttcgagc aaatggagat
    10381 gcagttcttc gtcaaacccg gcaccgatat ggagtggttc gagagctgga aagaggaccg
    10441 caagaactat tattccgccc tcggcatcaa tccggccaag acccgtttcc accagcacgg
    10501 cgaggacgag ctcgcccatt atgccgcggc cgccttcgat atccagtacg agtttccctt
    10561 cggctggaag gagctggagg ggatccacaa ccgcaccgat tttgacctca agcgccacca
    10621 ggagtacgca ggcgtcgacc tgagctactt cgacgaggtg acccgcgagc gctatgtgcc
    10681 ctacatcatc gagacctcag ccggcgtgga ccgcaccatg ctctgcatcc tctgtgacgc
    10741 ctttgaggag caggagctcg agaaagatac ccgcactgtc ctccacttcc atccggccat
    10801 cgctccggtc aaggccggca ttttcccgct ggtcaagaag gagggcatgc cggagatcgc
    10861 ccaccagatc gaggccatgc tgcagcccta ttacgccgtc ttttacgacg agagtggagc
    10921 agtcggccgc cgttatcgcc gccaggacga agtgggcacc cccttctgca tcaccgtt
//
LOCUS       DAOPWU010000090        10961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6046_length_10961_cov_5.231065, whole genome shotgun sequence.
ACCESSION   DAOPWU010000090 DAOPWU010000000
VERSION     DAOPWU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10961
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6046_length_10961_cov_5.231065"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKM23_06435"
     CDS             <1..542
                     /locus_tag="PKM23_06435"
                     /inference="COORDINATES: protein motif:HMM:NF028211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNY91147.1"
                     /translation="EQGVSGLLSILSVKYTTARGVAEAAVRAVGRKLGKKIDGKAGRK
                     RHVWGGDVGDFAGFLAVQQAERPEPVSEVQMEQLARNYGTGVGEVFKAGGGEREWLRP
                     VAGQERVLRAEVLHAVRHEMALTLADVVLRRTELGSGEYPGDEAAADAAAIMAAELGW
                     DAQRMQRELADLRRLYQPA"
     gene            639..1460
                     /locus_tag="PKM23_06440"
     CDS             639..1460
                     /locus_tag="PKM23_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY91148.1"
                     /translation="MADTIGSYLWKGSDEKTIDEVRRYWTDNVNTTQFWTGDPRQIGS
                     PEFYDTVGAFIRKNYEHRYRLIASEAAKYPGGRMLEIGCGAGWELVAWAQAGMQVSGI
                     DLSSGALELARGNFEHNKLEAELKLGNAEQLPFEDNTFDIVASFGVLHQTQSTENAVA
                     EVKRVLKPGGEAAITLYYKYSWKILLTQLGRVNFEFAHEDAPITRLYDKKQLRELFKE
                     YSSVEVFLDYTRATPSPRKGGLAWFFNHLFVPVYNLLPGCIRNKFGHAAVVLAKK"
     gene            1479..2462
                     /locus_tag="PKM23_06445"
     CDS             1479..2462
                     /locus_tag="PKM23_06445"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNY91149.1"
                     /translation="MRALVTGASGFTGGYMVDHLVERGYTVRAFVRPTSDTARLKAKG
                     VDIITGDLKYKADLAQAMRNIDVVYHIAALYREANQPDQAYWDVNVTGTENVMAAALE
                     MGVRRVVHCSTCGVHGHIEKPPADEKAPIKPGDIYQETKAEGEKVALFYNREKGLPVT
                     IVRPVGIYGPGDERMLKMYRMIYRGKFIMFGGGNVLYHLSYVTDVVEGFRLAGETPAA
                     AGETYIIAGEKYFTLNDFARMVAQALEVRPPRLHPPVWPVYAAGWLCEKICIPLRVQP
                     PIFRRRVDIFVKDRAFDISKAKRELGFQPRVELEHGIRRTAYWYKENGLLD"
     gene            2489..3661
                     /locus_tag="PKM23_06450"
     CDS             2489..3661
                     /locus_tag="PKM23_06450"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNY91150.1"
                     /translation="MKTINVIGNFSGRNAGDAAILGGVLEDVSRIYPDALFTIPTINP
                     GFVQRQFAEYRVQTVSMLPWNGSMKILGVPVLRAALSADVILVTDAILFDRKLYNPLF
                     NYLWTLSRFLPRAFRKGVPVVLYNCSLGPIRTQAGHAALQAILDSTSALILRDRESVE
                     LLDRQGFHHPRVIEGADCALNARPVDEARLDEICREADLFSSGRPVIGFNVNSYVDAF
                     VRQGGTFGRENLVELYAATVDKVITELDVDVLFVETQHMDMGIASAVLGKIHQRERVR
                     LISNKVYSYREICAVLQKLQLFAGMRTHSLILSSAMGVPPVGIVTYPKNRGYMRTIGM
                     EAHLVDFKELAVESFYARISAAWHERERIREAMLPQVAREKEKARRSAEVLREYLG"
     gene            3717..5486
                     /locus_tag="PKM23_06455"
     CDS             3717..5486
                     /locus_tag="PKM23_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HNY91151.1"
                     /translation="MSAKKPLSVLLRWLISIALIAWVLYKVDPGKLWQTLKQVDWFYF
                     SLSIALTPVLIWLSSWKWQVILRAQGITASGWRLFWLYMVGYFFNTVLPTNVGGDVVR
                     AWALGKSTGENAKAYASVFIERFTGLTALILLAVAAFLAALGKLWDPWLALAMGVAVA
                     GYLALLVVVLNERYLAWLERKAPAGILHKIILKLKKFQEAILSIRSERGALLFAMANS
                     FLFYLAAVVNVWVSALAFRSPLSFIDALIITPIVMVIMMLPISIGGIGLAEWAYFFTF
                     DRMGLTGAVGLSVALLMRVKALATGILGGIYYSTLGIRIDQEVKMGREPEREIGAGDV
                     AGEVRYYSGFEDVMRRRKSPLHKYADIVIGPLRLWRLFKYEFITLFCMPLPGMIGYGL
                     RQIFLPAVLRKQGKGAVWSRNVTLRHPHKIAVGKRCVIEEYCSLSAQGDEQSGITLGE
                     EVLLGRGTVLGTRNGTIEIGDYSNIGANCRLGTTTRIRFGRHVLLAANCYIGGAQHKF
                     DRLDVPIMRQGYESKGGVVIEDDCWLGAGVMVLDGVTIGTGSVIGAAAVVTKDIPPYS
                     IALGIPARVVGSRSSSDTPNPTS"
     gene            complement(5476..9606)
                     /locus_tag="PKM23_06460"
     CDS             complement(5476..9606)
                     /locus_tag="PKM23_06460"
                     /inference="COORDINATES: protein motif:HMM:NF020698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY91152.1"
                     /translation="MRIRLLALFCLAAVVAAAQTVPEHKRLLYSDSLTNASSTRMGTL
                     ENRGGEFIPGRGWKISGQYSQLTIALPADLPPEGTFKINVTNFDPASQSVDEKQNLLT
                     MASDDSIYWLPQYETGSWWLYRTGKGYTDGPGMAGFRIDYAPNGVDTRSDGRAMQSTK
                     WSLNKTYEFKVVWQANWISFYVDNVLIMDPKISKTPWTGQKELFRYILIGRNSKNYPA
                     QPGPIWSNARIYVPGSDLSLTKVSGDNQNGAAGSLLASSVVVKVVDADGQPVPNEPIQ
                     LQVISGGSIDNLPAVIKTTNDKGMVSADWILGPAVGLQHLRATHGDSVIEFSATATPS
                     HNRRLLLVSGGGQNGTPGQPLPEPIVVQVRDEYDNPVSGQSLIFTIAAGGGSLAGQAG
                     ISAFTDSSGRAAIMWTMGLLRGSVQRLEISGSSGEPLTGSPLTVEAGLGAIPDQALST
                     ITATSPVTANGRDHSAVTVTLRDGSGMPLSGYVVRLTVSEAGAILTAADSLTNASGQI
                     FATLASTTPGSATVSAHVIGADLHLNSTAVVRFDPVPQSAGRIVMLSGDGQTGIVATV
                     LPGLLKVQVLDDKEQALAHYAVEFFVISGDASFDGQARLTTTTNEEGVASARLSLGET
                     YGTITAGATTAPLTTVITFKARAVSGAAYQMVAESGFNQNGPPGQPLPRPVTVSVRDH
                     FGNPAPDHPLLFKIISGGGSIAGSNQAVIRTDAEGSASVSWTLGRYLGAANRLEISNA
                     SEIIVSLRGSPLIMQVPQPALPDITISTVTASSPVIADGAAYSEVTVTLADNLSRPLA
                     GFTVDLFASGENNLITLADSLSDRDGQVTARLRSTTPETKIIGASVRGAGLFLNDTAR
                     VDFIAPSKMDQLLYISGDGQAGRIGALLPEALVVRALDHTNTPKGGVAVEFTVDAGGG
                     LVNRRTKSTVFSDTAGYARMLWSLGSDAGERNNHVTARIRGAFSAPITFTATALAADP
                     AQWFILSGNHQTGVAGRALAEPFRIGVRDQNGNDVSDFPILFRTIRGGGRFVDAQEYG
                     TRTGPTGQASAQFTLGERAELQQVQVFSVTAPLESLLFEATATPEAPDRLVRVGAESL
                     TVSLADPEAVELAVRLVDRYANPVPGVEVDISAPDGGHVLSQPGALTDTKGEAYFQVS
                     PGELPGAYHFACRSVGDITSLFYLTAFLPQVNQAPLITAFSPADTTLIDITPGMSLEF
                     RFDEVADPDGDTLFFQWMVNGVPATTRNALSLVVNPTLIPQDGAALRVTGSVSDGEET
                     VTIGWRFRIIMTGVKDGGQEMPRVVQMEQNYPNPFNSSTTLAVALPGRSTASLRIYSL
                     SGQMVTVLFEGILAEGNHRFVWQGMNQRGEPMPSGVYYGILQAGEVRQTRKIVLMR"
     gene            9907..>10961
                     /locus_tag="PKM23_06465"
     CDS             9907..>10961
                     /locus_tag="PKM23_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY91153.1"
                     /translation="MALRSKWIGILVTVSLTWALAAGAQTVNKVLELEDSLKGATKGA
                     QKGGKLTAEGWVTQTAFDFIEYKIPSCSAGEVEFKVKGIHASREVFPNVGYDRDGKPV
                     PGSDNVHYTLFGMYDRDEDNSWYGSVQWHNPYKTVVHIYGYTEGDLYKWKRMKLRLNV
                     AAFNGGYEDDPHAFEDPMVGPFEWQKEHTYHHRLVWGSGHMLWYLDGVLIKDWDYSSF
                     GVEYAPPDHTLRLGSGLNSRSGGYASPNDLTFTDLKFYRHKDETRPQVMRLDPAAGSS
                     AVAPDADILVHFSEPMDEGSAQAAFSITPALAGSFRWIGSALALEHSALLQPNTTYTV
                     RVEATARDKSGLNLTAP"
BASE COUNT         2039 a         3300 c         3512 g         2110 t
ORIGIN      
        1 aggagcaggg ggtgtccgga ctgctcagca ttttgagcgt aaaatatacg accgccaggg
       61 gtgtggccga agccgcggtg cgggcggtgg gccgcaagct cggcaaaaag atcgatggca
      121 aagcgggccg caagcggcat gtgtggggag gggatgtcgg ggatttcgcc ggctttctcg
      181 cggtccagca ggcggagcgg cccgagccgg tcagcgaggt gcagatggag cagctggccc
      241 gcaactacgg caccggcgtc ggcgaggttt tcaaggccgg cgggggggag cgggagtggc
      301 tgcggccggt ggccgggcag gagcgggtgc tgcgcgccga ggtgcttcac gccgtccgtc
      361 acgaaatggc cctgaccctg gccgatgtgg tgctgcgccg cacggagttg ggctcgggcg
      421 agtatccggg cgatgaggcg gcggccgatg cggccgcgat catggcggct gaactgggct
      481 gggatgcgca gcggatgcag cgcgagctgg ccgatctcag acgcctctat caaccggcgt
      541 agatttcctg ctttgaaaat tgcgcaaaat tgtgtaccgt gtagggaaag ccagtaaata
      601 attaattatt caccaatgat caggaacttg aggtttttat ggcagatacc atcggctctt
      661 atctgtggaa gggcagcgac gaaaagacca tcgacgaggt gcgccgctac tggacggaca
      721 acgtcaatac cacccagttc tggacggggg atccccgcca gatcggctcc cctgaattct
      781 acgacacggt gggggctttc atccgcaaga actatgagca tcgctaccgg ttgatcgcca
      841 gcgaggcggc caaatatccc gggggcagga tgctcgagat cggctgcggc gcgggctggg
      901 agctggtcgc ctgggcccag gcggggatgc aggtctccgg catcgatctc tcctcggggg
      961 cgctcgagct ggccaggggc aatttcgagc acaacaagct cgaggccgag ctcaaactcg
     1021 gcaatgcgga gcagctcccc ttcgaggaca acaccttcga catcgtcgcc tccttcggcg
     1081 tgcttcacca gacccagagc accgagaacg cggtcgccga ggtcaagcgg gtgctcaagc
     1141 ccggcggcga ggcggccatc accctctact acaagtactc gtggaagatc ctgctgaccc
     1201 agctcgggcg ggtcaatttc gagttcgccc acgaggatgc cccgatcacc cgcctctacg
     1261 acaaaaaaca gctgcgcgag ctcttcaagg agtacagctc ggtggaggtt tttctcgact
     1321 acacccgggc caccccttct ccgcgcaagg ggggactggc gtggttcttc aaccatcttt
     1381 tcgtgccggt ttacaatctg ctgcccggct gtatccgcaa caagttcggc catgccgcgg
     1441 tcgtgctggc gaaaaaataa gccaaaggga gaaagacgat gagggctctg gtcaccggcg
     1501 cctccggatt caccggcggc tatatggtcg atcatcttgt agagcgggga tatacggtgc
     1561 gcgcctttgt gcggcccacc agcgacaccg cgcgcctcaa ggcgaaaggg gtggacatca
     1621 tcaccgggga tctgaagtac aaggcggatc tggcccaggc gatgcgcaac atcgatgtgg
     1681 tctatcacat cgctgcactc taccgggagg ccaatcagcc cgaccaggcc tactgggacg
     1741 tcaatgtcac cggcacggag aacgtcatgg ccgcggccct cgagatgggg gtgcgccggg
     1801 tggtgcactg cagcacctgc ggcgtgcacg gacacatcga aaagcccccg gccgatgaaa
     1861 aggccccgat caagcccggg gatatctatc aggagaccaa ggccgagggc gaaaaggtgg
     1921 cgctctttta caaccgggag aaggggctgc cggtcaccat cgtcaggccg gtgggcatct
     1981 acggacccgg cgacgaacgc atgctcaaga tgtaccggat gatctaccgg ggcaagttca
     2041 tcatgttcgg cgggggcaat gtactctacc atctcagcta cgtcaccgac gtggtcgagg
     2101 gcttccgcct cgccggcgag actcccgctg cggcaggaga gacctacatc atcgccgggg
     2161 agaagtattt cacccttaat gacttcgccc ggatggtcgc tcaagccctc gaggtcaggc
     2221 cgccgcgatt gcatcccccg gtctggccgg tctatgcggc cggctggctc tgcgaaaaga
     2281 tctgcatccc cctgcgggtg cagccgccga tcttcaggcg gcgcgtcgac atctttgtca
     2341 aggaccgggc ctttgatatc agcaaggcga agcgggagct cggcttccag ccccgagtgg
     2401 agctggagca cggcatccgc cgcacggcct actggtacaa ggaaaacgga ctcttggatt
     2461 aatgctgaat caaaatcaag cgggtgttat gaaaaccatc aatgtcattg gcaatttttc
     2521 cggccgcaat gcgggcgacg ccgccatcct cggcggggtg cttgaggatg tctccaggat
     2581 ctatccggac gcccttttca cgatcccgac catcaatccc ggattcgtgc agcgccagtt
     2641 cgccgaatac cgggtgcaga cggtctcgat gctgccctgg aacggctcga tgaagattct
     2701 cggggtgccg gtcctgcgcg ccgccctctc ggcggatgtg atcctggtga ccgatgccat
     2761 tcttttcgat cgcaagctct acaatcctct cttcaactat ctctggaccc tttccaggtt
     2821 ccttccccgc gccttcagaa agggagtgcc ggtggtgctg tacaactgca gcctcgggcc
     2881 gatccggacc caggcgggcc atgcggccct gcaggccatc ctcgacagca ccagcgccct
     2941 catcctgcgc gaccgggagt cggtggagct gctcgaccgc cagggatttc atcatccgcg
     3001 ggtgatcgag ggggccgact gcgccctcaa cgccaggccg gtcgatgagg cgcggctgga
     3061 tgagatctgc cgtgaagctg atctcttcag cagcggccgt ccggtgatcg gctttaatgt
     3121 caacagctat gtcgatgcct ttgtccgcca gggcggcacc ttcggccgcg aaaacctggt
     3181 cgaactctat gccgccaccg tcgacaaggt gatcacggaa ctggatgtgg atgtcctctt
     3241 cgtcgagacc cagcatatgg atatgggcat cgcctccgca gtgctcggca agatccatca
     3301 gcgcgagcgc gtccgtctca tttccaacaa ggtctacagt tatcgggaga tctgcgcggt
     3361 gctgcaaaag ctgcagctct ttgcggggat gcgcacccac tccctgatcc tctcctcggc
     3421 gatgggagtt ccgccggtgg gcatcgtcac ctatcccaag aaccggggct acatgcgcac
     3481 catcggcatg gaggcccatc tggtcgattt caaggagctg gcggtggaga gcttttacgc
     3541 ccgcatcagc gcggcctggc acgaacgcga gcgcatccgc gaggcgatgc tgccacaggt
     3601 cgcccgtgaa aaggaaaagg cgcgccgctc cgccgaggtg ctcagggagt acctgggata
     3661 accccttgca tggcgcccgg cgagaccggg cgctctcttg agagagtcca tcgatcatgt
     3721 ccgccaagaa accactctcc gttctcctgc gctggctgat cagcatcgcc ctgatcgcct
     3781 gggttctgta caaggttgat cccggcaagc tgtggcagac gctcaaacag gtggactggt
     3841 tttatttcag cctctcgatc gccctcaccc cggtgctgat ctggctcagc tcctggaagt
     3901 ggcaggtcat cctgcgcgcc cagggcatca ccgcctcggg ctggcgcctc ttctggcttt
     3961 acatggtggg ttattttttc aataccgtgc tgccgaccaa tgtcggaggc gacgtggtgc
     4021 gcgcctgggc cctgggcaaa tcgacggggg agaacgccaa ggcttatgct tcggtcttta
     4081 ttgaacgttt caccggtttg accgcactca ttctcctggc ggtggctgct tttctcgccg
     4141 ctctgggcaa gctctgggat ccctggcttg ccctggccat gggggtggcg gtggccggct
     4201 atctcgccct gctggtggtc gtcctcaacg agcgttatct cgcctggctg gaacgcaagg
     4261 ccccggccgg cattctgcac aaaatcatcc tcaagctgaa aaagttccag gaggcgatcc
     4321 tctccatccg cagcgagcgc ggcgccctcc tcttcgcaat ggccaactct ttcctctttt
     4381 atctggcggc tgtggtcaac gtctgggtga gcgccctcgc cttccgttcc cccctctcct
     4441 tcatcgatgc cctgatcatc accccgattg tgatggtgat catgatgctg cccatctcga
     4501 tcggagggat cggcctggcg gagtgggcct acttcttcac ctttgaccgc atgggtttga
     4561 ccggtgcggt gggcctctcg gtggccctgc tcatgcgcgt caaggccctg gcgaccggga
     4621 ttctgggcgg catctactat tcaaccctgg ggattcgcat cgatcaggag gtcaagatgg
     4681 ggcgggagcc ggagcgcgag atcggcgccg gggatgtggc cggcgaggtc aggtattaca
     4741 gcggctttga ggatgtcatg cggcgccgca agtcgcccct gcacaagtac gccgacatcg
     4801 tcatcggccc gctcaggctg tggcgcctct tcaaatacga attcatcacc ctcttctgca
     4861 tgcctctgcc ggggatgatc ggctacgggc tgcgccagat cttcctgccg gcggtgctgc
     4921 gcaagcaggg caagggggcg gtctggagcc gcaacgtcac cctgcgccac ccccacaaga
     4981 tcgctgtcgg caagcgctgc gtcatcgagg agtactgctc gctttcggcg cagggggacg
     5041 agcagagcgg catcaccctc ggcgaggagg tgctgctcgg gcggggcacg gtgctgggca
     5101 cgcgcaacgg aacgattgag atcggggatt acagcaacat cggcgccaac tgccgcctgg
     5161 ggacgacgac gcggatccgc ttcggccggc atgtgctgct ggcggccaac tgctacatcg
     5221 gcggggccca gcacaagttc gaccgcctcg atgtgccgat catgcgccag ggctacgaga
     5281 gcaagggggg ggtggtgatc gaggatgact gctggctcgg agccggggtg atggtccttg
     5341 acggagtcac catcggcacc ggctcggtga tcggggcggc ggcagtggtg accaaagata
     5401 tcccgcccta ttcgatcgct ctgggcatcc ccgccagggt ggtgggaagc cgatcctcct
     5461 ccgacacccc caatcctacc tcataagtac gatttttctg gtctgccgca cctctccggc
     5521 ctgcaggatg ccgtaataga ctcctgaagg cattggctca cctctctgat tcatcccctg
     5581 ccagacaaag cggtggttcc cctccgccag gatcccctcg aacaatacag tgaccatctg
     5641 accggaaagg ctgtagatgc gcagcgaggc tgtgctgcgg ccggggaggg ccaccgccag
     5701 cgtagtcgag ctgttgaagg gattcggata gttctgttcc atctgcacca cccgcggcat
     5761 ctcctgcccg ccgtctttca ccccggtcat gattatcctg aagcgccacc cgatcgtcac
     5821 ggtctcctcg ccgtcgctca cgctgccagt aacacgcagg gcggcgccgt cctggggaat
     5881 gagggtagga ttgacgacca gggagagtgc attccgggtg gtggcgggca cgccattgac
     5941 catccactga aagaagagcg tgtcgccatc cggatccgcg acctcatcga accggaattc
     6001 cagactcatg ccgggtgtga tgtcgataag ggtggtatcg gccggggaga aagcggtgat
     6061 gagcggcgcc tgattaactt gcgggaggaa ggcggtcaaa taaaagagag aagtaatatc
     6121 gccgacgctc cggcaggcaa agtgataggc gcctgggagt tcacccggcg agacctgaaa
     6181 gtacgcctcg cctttcgtat cggtgagagc gccgggctgt gagagcacgt gtcccccatc
     6241 cggggccgaa atgtccactt ccacgcccgg cacaggattg gcgtagcggt ccaccagccg
     6301 cacagccagc tccactgcct ccggatcggc cagagagaca gtcaggctct ctgcgcccac
     6361 cctgaccagg cggtccgggg cttccggcgt cgccgtcgct tcgaagagca gggattcgag
     6421 cggggctgtg acggaaaaga cctgaacctg ctggagctcc gcccgttcgc ccagggtgaa
     6481 ctgggctgac gcctgccctg tgggaccggt gcgcgtaccg tattcctgcg catcaacaaa
     6541 gcgccccccg ccgcggatgg tcctgaagag gatcggaaaa tcgctgacgt cattgccgtt
     6601 ctggtcgcgg accccgatgc ggaagggctc cgccagggcc cggccggcca ctccggtctg
     6661 gtggttgccg ctgaggatga accactgcgc cggatcggcg gccagggcgg tggcagtgaa
     6721 ggtgatcggc gcggagaacg ccccgcggat gcgggcggtg acatggttgt tccgttcccc
     6781 ggcgtcggag cctaggctcc agagcatgcg cgcgtaaccg gcagtgtccg aaaacaccgt
     6841 gctcttcgtc cgtctgttca ccagcccccc gccggcgtcg accgtgaatt cgacggcgac
     6901 accgcccttc ggggtgttcg tgtgatcaag ggcccgcacc accagcgctt cgggcaggag
     6961 ggcgccgatg cgccctgcct gtccatcgcc ggagatatag agcagctggt ccatctttga
     7021 tggagcgatg aaatcgaccc gggctgtgtc gttgagaaag agccccgcac ccctgacgga
     7081 ggcgccgatg atcttggttt caggggtggt gctgcgcagc cgggcggtga cctgcccgtc
     7141 gcggtcgctc agggagtcag ccagggtgat caggttgttc tctcctgagg cgaagaggtc
     7201 gaccgtaaag cccgcgagcg gtcgggatag attatcggcc agggtgacgg tgacctcgga
     7261 ataggcagcg ccgtcggcga tgaccgggct ggaagccgta accgtggata tggtgatatc
     7321 cggcagcgcc ggctgcggga cttgcatgat aaggggtgat cccctgaggg aaacgatgat
     7381 ttcactggcg ttgctgatct ccagccggtt cgccgcgccc agatatcttc ccagggtcca
     7441 cgagacagag gcgcttcctt ctgcatcggt ccgtatcacc gcctggttcg atcctgcaat
     7501 gctgccgcca cccgatatga tcttgaaaag cagggggtga tccggtgctg gattaccaaa
     7561 gtgatccctg acgctgaccg tgacaggtcg ggggaggggt tggccgggag gaccgttctg
     7621 gttgaaaccg ctctctgcaa ccatctgata ggctgctccg ctcacagctc tggccttgaa
     7681 agtgatgacc gttgtcagag gggcggtagt ggcgcctgca gtaatggtgc cataagtttc
     7741 gccgagggag agtctggcgc ttgcgactcc ctcctcgttg gtcgtcgtgg tgagccgggc
     7801 ttgtccgtcg aaggaggcat caccggaaat gacaaagaat tcgaccgcat aatgcgccaa
     7861 cgcctgctct ttatcgtcca ggacctggac tttcagaagc ccgggcagca ccgtggcgac
     7921 gatgccggtt tggccgtccc ccgagagcat gacgatgcgg ccggcacttt ggggtactgg
     7981 atcgaatcgg acaacagctg ttgagttgag gtggagatcg gccccgatca catgcgcgga
     8041 gacggttgcg gagcccgggg tagtgctcgc cagggtggcg aaaatctgcc cggacgcatt
     8101 cgtgagcgag tcggctgccg tcaggatggc gccggcttca ctgacggtga ggcggacaac
     8161 gtagccgctg agcggcatgc cgctgccgtc gcggagtgtg acggtgacgg cggagtgatc
     8221 cctgccgttg gcagtgaccg gcgaggtcgc cgtgatggtc gagagcgcct ggtccggaat
     8281 cgccccgagg cctgcctcca cagtgagcgg tgagcccgtc aggggctcgc cgctgctgcc
     8341 ggagatttcc agcctttgca cactgccgcg caggagcccc atagtccaca taatcgcggc
     8401 ccttccgctg ctgtcggtga acgcactgat gccggcctgg cccgccagag agccgcctcc
     8461 ggctgcaatc gtgaagatga ggctttgacc ggaaaccgga ttgtcatatt catcgcgcac
     8521 ctgaacaact atgggctcgg ggagaggctg gccgggtgtg ccattctgcc ctccgccgct
     8581 gacaagcagc agcctccggt tatgggaggg tgttgcggtg gcgctgaatt cgatgaccga
     8641 gtccccatga gtcgcgcgca gatgctgtaa ccccacggcc ggacccagga tccagtctgc
     8701 gctgaccatc cccttgtcgt tggtggtttt gatcacggcc ggcagattat caatactgcc
     8761 gccgctgatt acctgcaact gaatgggctc attggggacg ggctggccat ctgcatccac
     8821 aaccttgaca accacgctgg aggcgagcag gctgccggca gcgccgttct gattgtcccc
     8881 ggaaaccttg gtgagcgaga gatctgaacc cgggacgtag atgcgcgcat tggaccagat
     8941 cggtcccggc tgggcgggat aatttttact gttgcggccg atcaggatgt agcggaagag
     9001 ttccttctgt ccagtccagg gcgttttact tatcttggga tccatgatca ggacattgtc
     9061 gacgtaaaag ctgatccagt tggcctgcca gaccaccttg aattcatagg ttttgttgag
     9121 actccatttg gtgctctgca tcgcccggcc gtcactgcgg gtgtcgacgc cattgggggc
     9181 gtaatcgatg cggaagccgg ccatccccgg tccgtcggtg tagcccttgc cggtgcggta
     9241 gagccaccag gacccggtct cgtattgcgg cagccagtaa atgctgtcat ctgaagccat
     9301 ggtgaggagg ttctgcttct catcgacact ctgggaggcg ggatcgaaat tggtgacatt
     9361 gatcttgaag gtgccctccg gcggcaggtc ggccggcagg gcgatggtca gctgactgta
     9421 ttgcccgctg atcttccagc cccgcccggg gatgaattcg ccgccccggt tctccagggt
     9481 ccccatccgg gttgaggagg cgttggtgag gctgtccgag taaaggagcc gcttgtgctc
     9541 cggaacagtc tgggcggccg ccactaccgc cgccagacag aagagggcta acaatcggat
     9601 gcgcattaaa ttctccggca gagatgggga aggtttttag aaaggagatg cggttcccaa
     9661 ggatgacagg ctgatattat gaatacgggc aggctcgctg gttcgcggaa aacggcgatt
     9721 tcgcgtcttt ctctgcctca aaaagggaat ttcatttaca ggattgtaac aaggagaccg
     9781 aggcctgttt gtaactcaag taaaaacaga cgcgatattc tggcccgctc tttgcatggc
     9841 cctgcccgga tattaataat gggtcatcct gaacactcac cccggtgtgg aatgggagga
     9901 agcaacatgg cgttacgcag caaatggatc ggcatcctgg ttacggtcag cctgacctgg
     9961 gcgctcgcag ccggcgcgca gacggtcaac aaggttctgg aactcgaaga ctcgctcaag
    10021 ggagcgacca agggggcgca gaagggcggt aaactcaccg ccgagggctg ggtcacccag
    10081 accgccttcg actttattga atataaaatc ccgagctgct ccgccggcga ggtggagttc
    10141 aaggtgaagg gcatccacgc ctccagggag gtctttccca atgtcggcta tgaccgcgat
    10201 ggcaagcctg tccccggttc cgataacgtg cattataccc ttttcgggat gtacgatcgt
    10261 gacgaggata acagctggta cggctcggtg cagtggcaca atccctacaa gaccgtggtt
    10321 catatctacg gttataccga gggtgacctc tataaatgga agcggatgaa gctgcgtctc
    10381 aatgtcgccg ccttcaacgg cggatatgag gatgatccgc acgccttcga ggatcccatg
    10441 gtcggtccct ttgagtggca gaaggagcac acctatcacc accgcctggt ctggggcagc
    10501 ggccacatgc tctggtatct ggatggtgtg ctcatcaagg attgggacta ttccagtttc
    10561 ggggtcgaat acgccccgcc cgaccacact cttcggctcg gctccggcct caacagccgc
    10621 agcggggggt acgcctcccc aaacgacctg accttcacgg atctcaagtt ctaccgccac
    10681 aaggacgaga ccaggccgca ggtgatgcgg ctggatccgg ccgccggcag cagcgcagtc
    10741 gctccggacg ccgatatcct ggtccatttc agcgagccga tggatgaggg ttcggcccag
    10801 gcggccttca gcatcactcc ggccctggcg ggctccttcc gctggatcgg ttcggccctg
    10861 gccctggaac acagcgccct gctccagccc aacaccacct acactgtgcg ggtcgaggcc
    10921 acggccaggg ataaatccgg cctgaacctg accgccccct a
//
LOCUS       DAOPWU010000091        10940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6058_length_10940_cov_4.725861, whole genome shotgun sequence.
ACCESSION   DAOPWU010000091 DAOPWU010000000
VERSION     DAOPWU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6058_length_10940_cov_4.725861"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="PKM23_06470"
     CDS             <1..134
                     /locus_tag="PKM23_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001986465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY91154.1"
                     /translation="LCDRVAIIHKGRLLFDGTIAAMRDHFKADESLEKMFLEMTEDE"
     gene            127..1782
                     /locus_tag="PKM23_06475"
     CDS             127..1782
                     /locus_tag="PKM23_06475"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91155.1"
                     /translation="MNRYLALSRILLKSSITPPASGRHERTKTALLMLFVAVSMLPLV
                     AALALTVSKLYDPLAAIGQQGVLLGFGCAISGLIIFIFGIFYTINVYYFAQDVEFLLP
                     LPLKAGEILGAKFTITLLFEYLTQLLILAPLLVTFGIRSGAGLSYYLLAAGVYLSLPV
                     IPLVLASLLAMALMSYSSIASHKDRFRIWAGGLGLIAALGVQFAMQKVMAGRKPEDFV
                     RLLQEGKNSLADWAVQLFPGVRLAVKALLQQGAAAGWLNLVLFLLLTGLFILILRLAG
                     GALYFRGAVGGSESYAAHRALNAAEMAEAGRSGGQLGALILKEIRLLVRTPAFFMNCV
                     LVNFIWPVMILFVMTSGEQEDIEGLRRLVGSNVSLATLLLAGIGAALLVAGVSGASAS
                     GISREGSQFFTSKYLPAPYLTQLKAKMTVAFLLALAGFALLLGVLAVLFQLPAAAIGL
                     LLLAALPAVAFPVLAGMLLDVHFPKLVWDNEYRAVKQNMSIVLLMVISGLAGAACIWM
                     ALRYSAAGWTPAVTAVLLLMAVDAGLYFMLAKKGADWLAAISA"
     gene            1881..2585
                     /locus_tag="PKM23_06480"
     CDS             1881..2585
                     /locus_tag="PKM23_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HNY91156.1"
                     /translation="MSKYDALKERVWRANMELPRHGIVIFTFGNVSAIDREAGVVAIK
                     PSGVAYEQLKADEIVVVDLEGRVVEGNLRPSSDTPTHLVLYQAFPAIGGIAHTHSTYA
                     VAWAQAARPIPLLGTTHADSLHIDVPCTDFMNAAAIEGGYETETGRQIVKTFARLSPG
                     EVEMVLVAGHGPFTWGATPEKAVQNSVLLEELARMACLTLQINPHASRLPQTLIDKHY
                     QRKHGPGAYYGQSGKV"
     gene            complement(2645..4966)
                     /locus_tag="PKM23_06485"
     CDS             complement(2645..4966)
                     /locus_tag="PKM23_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018962727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNY91157.1"
                     /translation="MTLPFSGQADAAGAAHPYWELLREMTDQCIDFSLNLRQSGHPGG
                     SRSKAHILIALLLGGAMRWDIRRPLLPLADRFILAAGHTNPLVYALLAVLNEALRCRA
                     KRGGRELYWPAGSEKQQLVWEDLLTLRRRGGLPGHAEMAGKSLFFKYNTGPSGHGAAA
                     AAGQALALKIAGHGGVRVFVLEGEGGLTPGIVQEAMNSAWGLGLNNLYLLVDWNNFGI
                     DAFPVSRVVHGSPAEWFGARGWRVIGTERGMEWEAVEDTLRELVSAEASSLRPNAAWF
                     RTRKGRGYGIYDAPSHGTPHTMNSPLFWEGRREFMERYGITFAGYGEPAPAGEEAQRE
                     QTRQNLARVLSLFEERPELAEYTAERLLELAGQVPATDESKVRARVSAAAVSEPGRDP
                     DLVDHKNYPPALFLAPGEKAANRQALAKFGAWVNALCKSRYGRPLFIAMSADLAESTN
                     IAGFARDWEGLPGCGLYDREANPSGALLPQEITELANAGICTGLASVNLAEAPADRYE
                     GFYGACSTYGAFSYLKYGPMRLFSQMAQDAPLKLGKILWVAGHSGPETADDSRTHFGI
                     FSPGVTQLFPAGQIINLHPWEHNEVPVLLSAALMSDAPIIALHLTRPAIEIPDRAALG
                     LPNHEAAARGAYLLRPFDSDLPPMGTIFVQGTMTTVNLLQLLPELRARGLNVRLVAAV
                     SHELFMLQTEEYRSSLISAEEWEYSTVISDGARVTMQPWLANHRAVRLAMTPDWDDRW
                     RSGGTVAEIMEEAHLSPEWLLRGIETFVEAVRE"
     gene            5218..5694
                     /locus_tag="PKM23_06490"
     CDS             5218..5694
                     /locus_tag="PKM23_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91158.1"
                     /translation="MKRLTLMLTLALSGLLFAGAFEQADAHGRHHVYIRIGPPAARIV
                     VRPASPWRDGIWIDGYWGWEHGRHVWIDGRWARPVRGHFWVDGRWVHTRHGWEWNPGY
                     WRKGDWRNNNNGRDRDWDRDRDRNRNRDNDRDRDNDRGRDNGRGRGDNRGDQNGRW"
     gene            5902..6774
                     /locus_tag="PKM23_06495"
     CDS             5902..6774
                     /locus_tag="PKM23_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNY91159.1"
                     /translation="MKRKIIKIDHEKCTGCGLCIPNCPEGAIQMIDGKARLVSDLLCD
                     GLGACLGHCPEGAIEVEEREAEPYDERRVMAMIIPQGENTIRAHLKHLKDHGETEYHR
                     QAVEMLRELNLPVPEAPAAPAHSGCPGSRSVDFKPAPAASPLTGPRHQAAAPAPPAGP
                     QPSRLSHWPIQLHLISPASSHFAGSDLLIAADCTAFALGGFHEGFLKGKTLIIACPKL
                     DSGLDLYEEKIRALIEQARVNTITVMIMEVPCCGGLLRIVQSAVANSSRKVPLKLVMV
                     SARGEILREEWALA"
     gene            complement(6783..8150)
                     /locus_tag="PKM23_06500"
     CDS             complement(6783..8150)
                     /locus_tag="PKM23_06500"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNY91160.1"
                     /translation="MITFPPGFNWGTATSSYQIEGAWREGGRGLSIWDAFAHTPGKTA
                     RGDTGDVACDHYHRFREDVALMARMGVKAYRFSIAWPRLQPAGYGPANPEGIRFYSDL
                     IDELLAHNITPWVTLYHWDLPLALQLEIDGWLDPRCAAFFRDYAALCFDHFGDRVKHW
                     ITFNEPWVAAVLGYGQGSFAPGRVSRDGPYQAAHQMLRAHALSVDLYRTRFAAQDGRI
                     GMAPNCDWREPLTPQAADREAAGRAVEFILGWFGDPLWFGEYPESMRARLGERLPRFT
                     DRESGLLRGSGDFFGINHYTTHYAADAPGKQPACVGCDPDADAEAAFSLDPAWPRTAM
                     GWAIAPWGLNRLLHWIDRRYGRPEIVITENGCAMEDRLEDGRVDDPGRIDFFKGYLAE
                     CSKAIGEGVRLSGYFAWSLLDNFEWASGYGKRFGLHYVDFATRQRLPKSSADWYRRLI
                     AANGF"
     gene            8402..10312
                     /gene="gatE"
                     /locus_tag="PKM23_06505"
     CDS             8402..10312
                     /gene="gatE"
                     /locus_tag="PKM23_06505"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HNY91161.1"
                     /translation="MPPFKSFEEMQPEDYAAIGFKSGLEIHQQLLTSKKLFCRCPAGR
                     YSDEYDAEILRHMRPTLSELGEYDGTALMEFKTKKEIIYRINRETVCTYEMDDTPPFE
                     LNEEALDIALGIAMLYNCVMVDELHIARKQYLDGSIPTGFQRTTIVGVDGAVPYRERS
                     IPIVQLGLEEDACREVSDFGHRRVYITDRLGMPLIETVTGPEMRTPLEVAEVAQLLRR
                     MVRSTGLVRTGIGAARQDVNVSVTGGTRIEIKGVSRIPQIPLLTYNEAMRQYNLLRLR
                     DELHRRGITPESFTGRYEVANKLLRKTRFQPIAQAIAAGNTVYGVVLQGFRDLLRWPT
                     QTDTFFAREISDRVRVIACLTTLPNIIHSDSPGETLASSEWLNLRKLLGAGEEETLVL
                     VWGPPADAETGAREILIRAREAAAGIPSETRQALEDGTNGFERILPGPDRMYPDTDLP
                     PKAVTPERRERIRTTLPAPVWEREVRYRSMGIPADTVEPLACSRWAGLFETAITQWGL
                     QPTLAAVVLIQYPRRLRKGQPGKSAEWSDEFMTALLKTVGAGRLQREGILPAMRHWLA
                     TGENLWESWPGMASENEIIGWIAEARVAECRSLAPAARCRLIMGRVMARAAGRANGAA
                     LAERVRRQEGVS"
     gene            10309..10788
                     /locus_tag="PKM23_06510"
     CDS             10309..10788
                     /locus_tag="PKM23_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNY91162.1"
                     /translation="MNLVTKEKRTFSRPMRLLSALMLLGEDVIKVPVFRCQRCGECIL
                     SHTGFVCSQRCPKRLRNGPCGGTGEGGTCEVYPERMCIWYLIYRRASLFGRLKMLYRV
                     EKIHNWNLEKTSAWLNVFVKRIEPPKWRVPKGEGWEEAEKEGPSGDSNEGSCAGEVK"
BASE COUNT         1975 a         3593 c         3472 g         1900 t
ORIGIN      
        1 ggctctgtga ccgggttgcc atcatccaca agggcaggct gctcttcgac ggcaccatcg
       61 ccgccatgcg tgaccacttc aaggccgacg aatccctgga aaagatgttc ctggagatga
      121 ccgaagatga atagatatct tgcgctctcc cggattctgc tcaaaagcag cattacgccg
      181 ccggcttccg gccggcatga acggaccaag acggctctgc tgatgctgtt tgtggccgta
      241 tccatgctgc cgctggtcgc cgccctggcg ttaacggtca gcaagctcta cgaccccctc
      301 gccgctatcg gccaacaggg cgtcctgctc ggcttcggct gcgccatctc gggcctgatc
      361 attttcatct tcggcatctt ttacaccatc aacgtctact atttcgccca ggatgtcgaa
      421 ttcctgctgc ccctgccgct caaggcgggg gagatcctcg gcgccaaatt cactatcacc
      481 ctgctcttcg aatacctcac ccagctgctc attctcgccc ccctgctggt gaccttcggg
      541 atcaggagcg gcgccgggct cagctattac ctcctcgccg ccggtgttta tctctccctg
      601 ccggtcatcc ctctggtgct ggcttccctg ctggcgatgg ccctgatgag ctactcctcg
      661 atcgcgagcc acaaggaccg cttccgcatc tgggccggag gactggggct catcgccgca
      721 ctcggggtgc agttcgccat gcagaaggta atggccggca gaaaaccgga ggatttcgtc
      781 cgtttgctcc aggagggtaa aaacagcctg gcggactggg cagtgcagct ctttccgggc
      841 gtcaggctgg ccgtcaaggc cctactccag cagggggcgg cggcaggctg gctcaatctc
      901 gtcctcttcc tgctgctgac cggcctcttc attctcatcc ttcgtctggc cgggggagcg
      961 ctctatttcc gcggggccgt cgggggctcg gagagctacg ccgcccatcg cgccctgaat
     1021 gcggcggaga tggccgaggc cggacgcagc ggcggacagc tcggcgctct catcctcaag
     1081 gagatccgcc tgcttgtgcg cacgccggcc tttttcatga actgtgtgct cgtcaatttt
     1141 atctggccgg tgatgatcct ctttgttatg acctcgggcg aacaggagga tatcgagggg
     1201 ctgcgcaggc tggtgggcag caatgtgtcg ctggccaccc tgctcctcgc cggcatcggg
     1261 gctgcccttc tggtcgccgg cgtcagcggc gccagcgcct cggggatctc ccgcgagggc
     1321 agccagttct tcaccagcaa atacctgccc gccccctatc tgacccagct caaggccaag
     1381 atgacggtgg cctttctgct cgccctggct ggctttgccc tgctcctcgg ggtgctcgcc
     1441 gtgctcttcc aactgccggc ggcggccatc ggcctgcttc tgctcgccgc cctgccggcg
     1501 gtcgcttttc cggttctggc cggcatgctc ctggatgtcc attttcccaa gctggtatgg
     1561 gacaacgagt accgggcggt caaacagaat atgagtatcg tcctgctcat ggtcatcagc
     1621 gggctggcgg gcgccgcctg catctggatg gccctcaggt acagcgcagc agggtggaca
     1681 ccggccgtga cagcggttct cctcctcatg gcggtggacg ccggactcta ctttatgctc
     1741 gcgaaaaagg gggctgactg gcttgcggcg atcagcgcct gagctgcgct gcaggttcag
     1801 cacggaccgg aggaatgatg aacccggaag cctttggcgg ccggagaggt taacagaggt
     1861 tatacagaag gggaataccc atgagcaagt atgatgccct gaaggagcgc gtctggcggg
     1921 ccaacatgga gctgccgcgc catggcatcg tcatctttac attcggcaat gtcagcgcca
     1981 tcgaccggga ggccggggtg gtggcgatca agccgagcgg ggtcgcctac gaacagctca
     2041 aggccgatga aatcgtcgtc gtcgatctcg agggcagggt ggtcgagggg aatctccgcc
     2101 cctcctccga caccccgacc catctggtgc tctaccaggc cttcccggcc atcggcggga
     2161 tcgcccacac ccattccacc tacgccgtgg cctgggccca ggccgcgcgg ccgatccctc
     2221 tgctcggcac cacccacgcc gattccctcc acatcgacgt cccttgcacc gattttatga
     2281 acgccgcggc catcgagggg ggctatgaga ccgagaccgg acggcagatc gtcaaaacct
     2341 tcgcccggct ttcaccgggg gaggtcgaga tggtcctggt cgccggccat ggccccttca
     2401 cctggggggc gaccccggaa aaggcggtgc agaacagcgt cctccttgaa gagctggcca
     2461 gaatggcttg cctgaccctg cagatcaatc cccacgcctc gcgcctgccg caaaccctga
     2521 tcgacaaaca ctaccagcgc aagcacggcc ccggcgccta ttacggccag agcggcaagg
     2581 tatagaccgg atcacagcat gatgctttgc ccgctgcgct ccgttttccg gccggcgcgc
     2641 cggatcactc ccgaacagcc tcaacaaagg tttcgatccc cctgagcagc cattccggcg
     2701 agagatgggc ctcctccatg atctccgcaa ccgtgccgcc gctgcgccag cggtcgtccc
     2761 agtccggggt catggccagg cggacggcgc ggtggttggc cagccacggc tgcatggtga
     2821 cgcgcgcccc gtcgctgatc accgtcgaat actcccactc ctctgcgctg atcaggctgc
     2881 tgcgatactc ctcagtctga agcatgaaca attcgtggct cacagcggcc accagacgca
     2941 cgttgagccc tcgcgcccgc agctccggca ggagctgtaa caggttgacg gtggtcatgg
     3001 tgccctggac gaaaatagtc cccatcgggg gcagatcgga atcgaagggg cggagcaggt
     3061 aggcgccgcg ggctgcggcc tcgtggttgg gcagaccgag ggcggcgcgg tcagggattt
     3121 cgatggccgg gcgggtcaga tgcagggcga tgatcggggc gtcgctcatc agggcggcgg
     3181 agagcagcac cggaacctcg ttgtgctccc aggggtggag attgatgatc tgacccgcgg
     3241 ggaagagctg ggtcaccccg ggggaaaaga tgccgaaatg ggtgcgcgaa tcgtctgcgg
     3301 tctcgggacc ggaatggccg gcgacccaga ggatcttgcc cagcttgagg ggagcgtcct
     3361 gggccatctg gctgaaaagg cgcatggggc cgtacttgag atagctgaaa gccccgtagg
     3421 tcgagcaggc gccgtaaaaa ccctcatacc ggtcggcggg cgcctccgcc agattgaccg
     3481 aggccaaacc cgtgcagatg ccggcgttgg ccagctcggt gatctcctgg ggcagaagag
     3541 ccccggaggg attggcctca cggtcataga gcccgcagcc ggggagcccc tcccagtccc
     3601 gggcgaagcc ggcgatgttg gtcgattcgg ccaggtcggc cgacatggcg atgaagaggg
     3661 gccggccgta tctcgacttg cagagcgcat tgacccaggc tccgaatttc gccagagcct
     3721 ggcggttggc ggccttttca ccgggggcaa gaaagagcgc ggggggataa tttttatggt
     3781 caaccaggtc tggatcccgg ccgggctcac ttaccgctgc cgcgctcacc ctcgcgcgta
     3841 ccttcgactc atccgtcgcg ggcacctgtc cggccagctc gagcagacgc tccgcggtgt
     3901 actcagccag ctccggcctt tcctcaaaga gggagagcac ccgggccagg ttctgccggg
     3961 tctgctcccg ctgcgcttcc tcgcccgccg gtgcaggttc gccatagccc gcaaaggtga
     4021 tgccgtacct ctccatgaat tcccgtctgc cttcccagaa aaggggactg ttcatcgtgt
     4081 ggggtgtccc gtgggaaggg gcatcgtata tcccatagcc ccggcccttg cgcgtcctga
     4141 accaggccgc attgggcctg agggaggagg cctccgcgga gaccagctcg cgcagggtat
     4201 cctcgaccgc ctcccactcc atcccccgct ccgtacctat cacccgccag ccgcgggccc
     4261 caaaccactc ggcgggactt ccgtgcacca cccggctcac cggaaaggcg tcaatgccga
     4321 aattgttcca gtcgacaaga agatacaggt tgttcagccc cagcccccag gccgagttca
     4381 tcgcttcctg aacgatgccc ggtgtgagcc ccccttcccc ctccagcaca aagacccgca
     4441 cgccgccgtg gccggcgatc ttcagggcga gggcctgccc ggcggccgcg gccgcgccgt
     4501 ggcccgaagg accggtatta tacttgaaaa agaggctctt gcccgccatc tcggcgtggc
     4561 cgggcagccc cccgcggcgg cgcagggtga ggagatcctc ccagaccagc tgctgtttct
     4621 ccgaacccgc cggccagtac agctcccggc cgccccgctt tgcgcggcag cgcagggcct
     4681 cattcagcac cgcgaggagg gcgtagacca ggggattggt atggccggcg gcgaggatga
     4741 aacggtccgc aaggggaaga aggggacggc ggatatccca gcgcatcgct ccgccgagga
     4801 gaagggcgat gaggatgtgg gccttggatc gcgaaccccc gggatggccg ctctggcgga
     4861 ggttgaggct gaaatcgatg cactggtcgg tcatctccct gagcagctcc caatagggat
     4921 gggcggcacc tgcagcatcg gcctggccgg aaaaggggag ggtcatgacg cgctctcctt
     4981 gatgggaagt gataaagcgg gccggctgca cggaggggta cacccctgta cccaggggta
     5041 cactctccga ggccgccggc gcaatagggt ggtttgtaag atataattaa ataatatttt
     5101 agaataaatg ccatactttt ctggatttct ggcaccaggg ttgcaaaagc aggagcagag
     5161 agcactgcgg gagctgctga tgaaggcaga cccgatgtca ctgaacagga ggttgatatg
     5221 aaacggctca ctctcatgct gaccctggcg ctgtcgggtc tcttattcgc cggcgctttt
     5281 gaacaggcgg atgcccacgg caggcatcat gtctatatcc gcatcggccc gcccgcggcg
     5341 cggatcgtcg tcagaccggc ctccccctgg cgggatggca tctggatcga tggctactgg
     5401 ggctgggagc acggccgcca tgtctggatc gacggccgct gggcccgtcc ggtccgcggc
     5461 catttctggg tcgacggccg ctgggtccat acccgccacg gctgggaatg gaaccccggc
     5521 tactggcgca agggtgactg gcgcaataac aacaacgggc gtgaccgcga ttgggaccgc
     5581 gacagagacc ggaatcgcaa ccgggacaat gaccgggatc gtgacaatga ccggggccgg
     5641 gacaacggcc gcggcagagg cgacaaccgc ggtgaccaaa acggccgctg gtaacccgaa
     5701 gcccggagtg cgggcctgaa gtaaaatgcc agggccttcg cctccggaag cggattcctc
     5761 agcccggctg cctgaacagc agccgggctt tttttgcctc tccgcgcggc ggccccggtt
     5821 tattccgttg gctctggccc ggaaattttg tacattagca ggaaaaacgg gggagtggca
     5881 ttcagcgcag agaaaggatc catgaagcgg aaaattatca aaattgacca tgaaaaatgc
     5941 accggctgcg gcctctgcat tcccaactgc ccggaggggg cgatccagat gatcgacggc
     6001 aaggcccggc tggtgagcga cctgctctgc gacggtctcg gcgcctgcct gggccattgt
     6061 cccgaaggcg ccatcgaggt cgaggagcgt gaggcggagc cttacgacga acgcagggtg
     6121 atggccatga tcatccccca gggagagaac accatccgcg cccacctcaa acaccttaag
     6181 gatcacggtg agactgaata ccaccgccag gcggtggaga tgctgaggga actgaatctg
     6241 cccgtgcccg aggcaccggc tgcgcctgcg cactccggct gtcccggctc gcgcagcgtc
     6301 gatttcaagc ccgcgccggc ggccagtccc ctcaccgggc cgcgccatca ggctgcggcc
     6361 cctgccccgc cggcgggccc ccagccctcc cggctcagcc actggcccat tcagctccat
     6421 ctcatttcgc ccgcctccag ccattttgcc ggctccgatc tgctcatcgc cgccgactgc
     6481 accgctttcg ccctgggcgg ttttcatgaa ggctttctca agggcaagac cctgatcatc
     6541 gcctgcccca aacttgacag cggcctggat ctctatgagg agaagatccg cgcccttatc
     6601 gagcaggcgc gcgtcaacac catcacagtg atgatcatgg aggtgccctg ctgcggcgga
     6661 ctgcttcgca tcgtgcagag cgccgtggcc aactccagcc gcaaggtgcc gctcaagctg
     6721 gtcatggtca gcgccagagg cgagatcctg cgcgaggagt gggctctcgc ctgaggtctg
     6781 gcctaaaatc cgttcgccgc gatcagacgg cggtaccagt ccgcagaact tttgggcagg
     6841 cgctgccggg tggcgaaatc gacatagtgc agcccgaagc gtttgccata gcccgaggcc
     6901 cattcgaaat tgtccagcag agaccaggcg aaatagccgc tcagccgcac accctcccca
     6961 atcgccttgc tgcattccgc cagatagccc ttgaaaaaat cgatcctccc cgggtcatcg
     7021 acacgcccat cctcgaggcg atcctccatg gcgcagccgt tttcggtgat gacgatctcc
     7081 gggcggccgt agcggcggtc gatccagtgc aggaggcggt tgaggcccca gggggcgatc
     7141 gcccagccca tcgcagtgcg gggccaggcg ggatcgagtg aaaaggcggc ctctgcatcg
     7201 gcgtcgggat cgcagccgac gcatgctggc tgtttcccag gcgcatcggc tgcatagtgg
     7261 gtggtgtagt ggttgatgcc gaaaaagtcg ccgctccccc tgagcagccc ggattcgcga
     7321 tccgtgaacc gcggcagccg ctctccgagg cgggcgcgca tgctctccgg atactcgccg
     7381 aaccagagag gatcaccgaa ccagccgagg atgaactcga cggcgcgccc ggccgcctcg
     7441 cggtcagccg cctgcggagt gaggggttca cgccagtcgc agttcggggc catgccgatg
     7501 cggccgtcct gggcggcgaa acgggtgcgg tagagatcga cgctcagggc gtgggcgcgc
     7561 agcatctgat gggcggcctg ataggggccg tcgcgggaga cccggccggg ggcgaaggaa
     7621 ccctggccgt agcccaggac cgccgccacc cagggctcgt tgaaggtgat ccagtgcttc
     7681 acccggtcgc caaaatggtc gaagcagagc gcagcataat cccgaaagaa cgcggcgcag
     7741 cggggatcga gccagccgtc gatttcgagc tgcagggcga gggggaggtc ccagtggtag
     7801 agggtcaccc agggggtgat gttatgggcg aggagttcgt cgatgagatc ggaataaaag
     7861 cggatcccct caggattggc cggaccatag cccgcgggct ggaggcgcgg ccaggcgatg
     7921 gagaagcggt aggccttgac ccccatgcgc gccatgaggg cgacatcctc gcggaagcgg
     7981 tggtagtggt cgcaggcgac gtcgccggtg tcgcccctgg cggtcttgcc cggggtgtgg
     8041 gcaaaggcgt cccagatcga gagcccgcgt ccgccctcgc gccaggcccc ctcgatctga
     8101 tagctggagg tggcggtgcc ccagttgaag cccggtggaa aggtgatcat gttgtcctcc
     8161 tgcggtatca gtctttttat cctgacggac ttgcctaaat ataaggattg gcgggggcgg
     8221 agaaaagcaa aaagtgtgcc ccgcccacca ccccctcgtc cccacccgcc tctgtccctt
     8281 ccccaccccc tcgtccccac ccgcctctgc gcttttgtct tgcgaatgaa atccaaaatc
     8341 aataagttta tccaagcatg ctgcagatgc agccttgaac tcaatcacag agccttcctt
     8401 tatgccgccg ttcaaatcct tcgaagagat gcagcccgag gattacgccg ccatcggctt
     8461 caaatcggga ctggaaatcc atcagcagct cctcacctcc aaaaagcttt tctgccgctg
     8521 cccggccggc cgctacagcg acgaatatga cgccgagatc ctgcgccaca tgcgcccgac
     8581 cctatccgaa ctcggcgagt acgacggcac cgccctgatg gagttcaaga ccaaaaagga
     8641 gatcatctac cgcatcaatc gcgagaccgt ctgcacctac gagatggatg acaccccgcc
     8701 cttcgagctc aacgaggagg ccctcgacat cgccctgggc atcgccatgc tctacaactg
     8761 cgtcatggtc gatgagctgc acatcgcccg caagcagtat ctcgacggct cgatcccgac
     8821 cggtttccag cgtaccacca tcgtcggtgt cgacggcgcg gtgccctaca gggagcgctc
     8881 catccccatc gtccaactcg gcctggagga ggatgcctgt cgtgaggtca gcgatttcgg
     8941 ccaccgtcgc gtctacatca ccgaccgcct cggcatgccc ctcatcgaga ctgtgaccgg
     9001 gccggagatg cgcacccctc tggaggtggc cgaggtggcg cagctgctgc gccgcatggt
     9061 ccgctccacc ggactcgtcc gcaccggcat tggcgccgcc cgtcaggatg tcaacgtcag
     9121 cgtcaccggc ggcacccgca tcgagatcaa gggggtttcg cgcattcccc agatcccact
     9181 gctcacctac aacgaggcga tgcggcagta caacctgctg cgcctgcgcg acgagctgca
     9241 ccgccgcggt atcacccccg agagtttcac cggccggtac gaggtcgcca acaagctgct
     9301 gcgcaagacc cgcttccagc ccatcgccca ggccattgcg gccggcaaca cggtttacgg
     9361 cgtcgtcctg cagggctttc gcgatctgct gcgctggccg acccagaccg acactttttt
     9421 cgcccgcgaa atctccgacc gcgtccgggt catcgcctgc ctgacgaccc tgcccaacat
     9481 cattcacagc gactcgccgg gagagaccct ggccagctcc gagtggctca acctgcgcaa
     9541 gctcctcggc gcgggcgagg aggagaccct cgtcctggtc tggggccccc cagccgacgc
     9601 cgagaccggg gcgcgggaga ttctcatccg cgcccgcgag gccgccgccg ggatcccctc
     9661 ggagacccgc caggcccttg aggacggcac caacggtttt gagcgcatcc tgcccgggcc
     9721 cgaccgcatg tatccggaca ccgatctgcc gcccaaggcg gtcacccccg aacgccggga
     9781 gcgcattcgc accaccctgc ccgcgccggt ctgggagcgc gaggttcgct accgcagcat
     9841 gggcatcccc gccgatactg tggagcccct cgcctgttcg cgctgggccg gcctctttga
     9901 aacagcaatc acacagtggg gtcttcagcc caccctggcg gcggtggtgc tgatccagta
     9961 tcctcggcgg ctgcgcaagg gccaaccggg gaagagtgcc gaatggagcg acgagtttat
    10021 gaccgctttg ctcaagaccg tgggcgccgg gcggctgcag cgcgagggca ttctcccggc
    10081 aatgcgccac tggctcgcca ccggggaaaa cctttgggag agctggccgg gcatggcctc
    10141 ggagaacgaa atcatcggct ggatcgccga ggcgagggtg gcggagtgcc gctctctcgc
    10201 tccagccgcc cgctgcaggc tgatcatggg ccgggtgatg gccagggccg ccggccgggc
    10261 caatggggct gcactggccg agcgtgtccg ccggcaggag ggagtgtcgt gaatctggtg
    10321 accaaagaga aaagaacctt ttcccgcccg atgcgcctgc tttcggcgct catgctcctg
    10381 ggcgaagatg tgatcaaggt gcccgtattc cgctgccagc gctgcggcga gtgcatcctc
    10441 agccataccg gttttgtctg ctcccagcgc tgccccaagc ggctgcgcaa cgggccttgc
    10501 ggcggcaccg gcgagggggg gacctgcgag gtctatcccg aacgcatgtg catctggtat
    10561 ctcatctacc gccgcgcctc cctgttcgga cggctcaaaa tgctttaccg cgtcgaaaag
    10621 atccataact ggaacctgga aaaaacctcc gcctggctga acgtctttgt caaacgcatc
    10681 gagccgccca agtggcgggt gcccaaaggg gagggctggg aggaggcgga gaaggagggg
    10741 ccctccggcg attcgaatga aggcagctgc gcaggggagg tgaaataggc catgagtctg
    10801 cgtgaaaaaa tcgccgccgg aaaatttgtc gtctgcgccg aactcggacc gccccagagc
    10861 tgcgatgatg gtgtgatccg taaaaaggcg ggctgcttca ggggggtggt cgacgccgtc
    10921 aacatcaccg acaaccagac
//
LOCUS       DAOPWU010000092        10925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6071_length_10925_cov_5.419503, whole genome shotgun sequence.
ACCESSION   DAOPWU010000092 DAOPWU010000000
VERSION     DAOPWU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6071_length_10925_cov_5.419503"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /gene="eutB"
                     /locus_tag="PKM23_06515"
     CDS             complement(<1..805)
                     /gene="eutB"
                     /locus_tag="PKM23_06515"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HNY91163.1"
                     /translation="MKLSALLFGKTYQFKDIKDVLAKANELRSGDVLAGIAAETSQER
                     VAAKYVLANLSLADLRNHPVIPYEEDAVTRVIQDAVNENVFAEIRNWTVGELREYVLC
                     DRTTPEDIQRLRRGLTSEMVAACTKLMSNLDLIYAAKRIPVVSKANTTVGLPGHFSAR
                     LQPNDPRDDLKSITAQIYEGLSYGVGDAVIGINPCIDSPENVKKLLICIQEIIDKWQI
                     PAQSCVLAHVSTQMAAIRDGAPAGLIFQSISGSEKGLKEFGITVELLDEA"
     gene            complement(820..2256)
                     /locus_tag="PKM23_06520"
     CDS             complement(820..2256)
                     /locus_tag="PKM23_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HNY91164.1"
                     /translation="MSETKKIDSVGIDIGTTTLQLIFSALELINRAPATQVPRYEFSE
                     RRILYESAIHMTPVHEDGSIDEEQLFALLQSEYARAGRTLNEIETGAIIITGESSKAS
                     NARRSIMTLADRLGDFVVATAGPHLESVIAGRGSGAADFSDSRSARVLNIDIGGGTSN
                     YVLFDNGRVIDTACLNVGGHLIETDPAGRVTRVREPASRVLRSLFPGSIPQNNALTRA
                     DLERTAQRMADLVVDLIEGLSSPLLEELLMTPPLKEPGKLDALFISGGVGECFYHPQL
                     TQGDPFYFRDLGPILADALRTHPRLQAYPVRLPRQTIRATVIGAGAYSLSLSGSTIWV
                     AYEKLPLRNIPVLHPAIDWQQPEPEIYGEILQAARRHDLDPGADLFAIALSAAMPVTY
                     RAVVQCASALARLYREYPDPAHPAIIISANDVGKVLGMELEPLIKPKALAVIDEVNTR
                     EGDYIDIGKSYFGGDIVPLTVKSLAFPS"
     gene            complement(2314..3549)
                     /locus_tag="PKM23_06525"
     CDS             complement(2314..3549)
                     /locus_tag="PKM23_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNY91165.1"
                     /translation="MKRFFLPIALLLIAVLFLGTSSVYALNKMNWSPRNYEVLSKFIK
                     DYGVGGKYYDKKNPPYVALDWDQTMAHLDCEEATFRHQLWNLKFKLSKEEFKALFKDE
                     VNGVKQLNADYKNIKLADINADIISAYEFLHDNYAGFGVGKMTIEEIRETPQFKDFIT
                     KMPFLYDGYCETAGIEAVYGYPWVLFFLANHSVEEVQALAKDAITFALNDKLTKVTWK
                     APCNFACKAGPIDYTYKSGLRVHPEMQDLTESMMKAGIDVYICSASLKQVVQTFGAPG
                     NFGYNIDPNKVIAMELEVKNNILLPQYKTGWVQTQRMGKVEAIKKVLGHRGDPIFSAS
                     DSDGDYEMITEFKGMKLALIFNRLKGGPIGKASKLAVEQMTEKTPRFILQGRNENTGL
                     LLPQSETISVGKSEAKLLK"
     gene            complement(3586..4653)
                     /locus_tag="PKM23_06530"
     CDS             complement(3586..4653)
                     /locus_tag="PKM23_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91166.1"
                     /translation="MYKKLTVTILLTLVVLIFASSVAQAEKLNDKLTFQTVLRYRYMV
                     EEPYTKATSNYFYFDRGYVTMRYKYSDNIAMRYTLDFNHRNYTDPQNGKLLNQGTNAR
                     IKYAYIQFKNFLGLPGLEMLFGNYEQPGAQSYHNTIENSWIDRFAYVSGTAFDNGYSS
                     AVTGAMATYNLPASLGVKGYARLAVANKNTYSYAKEDNKAKNVIADIWLKPVAGLSVF
                     GWTMTDYYNKASNKDNNFWIGGGAEYAIDKKLDVGVEVTTGKKNVEAVPELDGVSGLT
                     YMGYVNYNILPNLMFVGEYGQLDPNGDVDNDEWNMILAGLNYKIIGENYLMFNVVRDG
                     FKVGDESKGNLRFVTQFWVEF"
     gene            complement(4728..5987)
                     /gene="eutH"
                     /locus_tag="PKM23_06535"
     CDS             complement(4728..5987)
                     /gene="eutH"
                     /locus_tag="PKM23_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005189864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HNY91167.1"
                     /translation="MALVNQIIIDIMMAFMVLGALDRILMQFGGSESVFKRVRLGRLG
                     RAFDGAGAQFEEGFNAMGPLALAMVGVISLAPVLARLLGPVIIPLYQFLGASPAMFAT
                     TLLANDMGGFFLAKELATTAGQIDYPAWMYAGLILGAMMGPTIVFSIPVGIGIIDKAD
                     RKHLALGVLAGIVTIPLGCIAGGLVAMISNIKAADGTAVIFTLGLIFKNLIPVVIVSL
                     LIALGLWRRPNAMIKGFLVFAKLLVALITLGLASIVLETLTGLKIIPGMDPIFPVSGD
                     QPGIDLRAIEVIGRIAIVLLGAYPMVYLLTRWFEKPLLSVGRLLKMNSTAAAGMIATL
                     ANNIPMFKLMSGMDDRGKVLNVAFAVSAAFTFGDHLGFTAANKPDMIFAVIVGKLIGG
                     ITAVLFAMLLAPKGSQAALEAAPPKGE"
     gene            complement(6026..7246)
                     /locus_tag="PKM23_06540"
     CDS             complement(6026..7246)
                     /locus_tag="PKM23_06540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNY91168.1"
                     /translation="MERGSEQRFIEEIVAQTVDEISVANHFTFRVPPLALVGDGVAAR
                     TGAEMKRIGASRVLIVTDQTILQLGLLDVVLRSLQRKGLSAEIFAQISGEPTWAMVNA
                     GTAMVKSAGCDAVLAVGGGSPIDAAKMIALLARNPLPFAEIDPDSELAQKPLPLAAMP
                     TTAGTASEVTDLAVIGDPASGRKVVFEHPALIPAVAIIDPLLTIGLPPAVTAATGLDV
                     LTHAIEAYVAQSSCTLARALSFRAIRLVATYLPIAVGYGTNTLARHKMAVACYMAGMA
                     FSNAGLGLCHAMSHQAGGRFKIPHGTANALILPHVMRFNLLVRAEEFGDIATALGERV
                     ENLNRREAAERAVTAVIHLIEDLGMPSGLAGFGVTDKDLPQLADQALQDSCLRTNPRT
                     AQKEDIIRIYQQAL"
     gene            complement(7249..7914)
                     /locus_tag="PKM23_06545"
     CDS             complement(7249..7914)
                     /locus_tag="PKM23_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91169.1"
                     /translation="MKKILPPFCLLLLIIAGCHSFVKPLQVDDPSFSKRDLHGKNILL
                     LPLTLIDIESSSSVISLFDKSDDLDQVRALIQQDLYQAFTAGPWKTIYWAEAAAADAA
                     PVLSVVQEKDYWRAAALDPARLTSAPQGFDFIVAPAALTITQEASDDVSMRSSDAPVR
                     GMAAKLDYAIFDRSGAVTLAHGRFEGVVARSGAFDEAVGRDPVKLAAREAIRLFIENL
                     RKQ"
     gene            complement(7937..8350)
                     /locus_tag="PKM23_06550"
     CDS             complement(7937..8350)
                     /locus_tag="PKM23_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY91170.1"
                     /translation="MDNSPHILIVDDDEYTRDSIDFILQSARFLVSSARNLGEALKKI
                     QLAESQGCPVQLVIIDLQMPVSGGLALLEELQHFRSYYALIAISAYGDTELRTGLRER
                     GCAGYLDKPFDDQQLIQAVTRGLKKKNHSSHVHLV"
     gene            complement(8571..10004)
                     /locus_tag="PKM23_06555"
     CDS             complement(8571..10004)
                     /locus_tag="PKM23_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY91171.1"
                     /translation="MKKITYPALPVLVVDDEEQFRLSLMMTLSSNGINNVIECGDSRA
                     VLGMLQETPCEMALLDLAMPGLSGSKLLPEIVKEYPHLPVIILTAINEVNSAVECMKC
                     GAFDYLVKPVDESRLVTTVRRAAELRQMQDENSRLKEYLLSNTLKNAEAFASTVTQNA
                     SMRSIFQYAEAVATTPLPVLITGETGVGKELIAQSVHKASGRSGQFIAVNVAGIDDTL
                     FSDTLFGHKRGSFTSAESDRKGLIEQAGDGTLFLDEIGELSQESQVKLLRILQEGRYY
                     PIGSDIPRLSNARFIVATNRDIEEMVQSGAFRRDLFYRLQTHRIHIPPLRERRDDIHL
                     LTDHFIRKACATLQKKVPAVPPELFILLSNYLFPGNIRELEGMILDTISRHQKGILSL
                     EAIREKVGNNGLPKNGETDPAAAIATAKRMAFGDSLPTMKEAGDLLIQEALARSRGNQ
                     KIAAMMLGITRRALNNRLHRKPDSLEK"
     gene            complement(10001..>10925)
                     /locus_tag="PKM23_06560"
     CDS             complement(10001..>10925)
                     /locus_tag="PKM23_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNY91172.1"
                     /translation="NAFDGRDSQYRGTPGSDADGRFLPYWNRITGKIAVEPLVNYQTD
                     DWYQIPKATGTSLLTEPYLYRGVLMATFSAPILREGKFTGVATGDVSLEYLSDMITRI
                     KLFESGFAFMVSNKGTIIAHKDRSLVGHKSLSEYGREAGGEGFIKLAEQIQRGEDGFI
                     ETEDPETHHPILIFFSPMKTGNWSFIAVVPRQEALAGVRSLTRAMLLIGLAALLLMTG
                     LVFLVATKITTPIKEVAERMKNAHINTLFNSTRPDEIGELTRAFDRFVTSVKSTILYI
                     AQSPGGQSSAPSLMGSKQKKNGPNAKPSEER"
BASE COUNT         2055 a         2897 c         3704 g         2269 t
ORIGIN      
        1 aggcctcgtc gaggagttcg acggtgatgc cgaattcctt gagccccttc tcggagcccg
       61 agatgctctg gaagatgagg ccggccggcg cgccgtcgcg gatggcggcc atctgggtgg
      121 agacgtgggc gaggacgcag ctctgggccg ggatctgcca cttgtcgatg atctcctgga
      181 tgcagataag cagctttttg acattttcgg gcgaatcgat acaagggttg atgccgatca
      241 ccgcgtcgcc gaccccatag gagagcccct cgtatatctg cgcggtgatg cttttgagat
      301 catcgcgcgg gtcattgggc tgaaggcggg cgctgaaatg gcccggcaga ccgacggtgg
      361 tattggcctt gctgaccacc gggatgcgtt tggccgcata gatgaggtcg agattggaca
      421 tcagtttggt gcaggccgcc accatttcgg aggtcaaccc ccggcgcaga cgctggatgt
      481 cctccggggt ggtccggtcg cagaggacgt actcgcgcag ttctcccact gtccagttgc
      541 ggatctcggc aaaaacattc tcattgacgg cgtcctggat cacgcgcgtg actgcatcct
      601 cctcataggg gatgaccgga tggttacgca gatcggccag actgaggttg gcgagcacat
      661 atttggcggc gacacgctcc tgcgaggtct cggcggcgat gccggcgagg acgtcgcccg
      721 agcgcagctc gttcgccttg gcgaggacat ccttgatatc cttgaattga taggttttcc
      781 cgaacagcag ggcgctgagc ttcatctgct tctcctcaat taggatggaa aggccaaaga
      841 tttgacggtc aggggcacaa tatcaccgcc gaaatagctt ttgccgatat ctatgtaatc
      901 cccctcgcgg gtgttcacct catcgatgac cgcgagggct ttgggtttga tcaggggctc
      961 gagttccatg ccgagcacct tgccgacatc attggctgag attatgatcg ccggatgggc
     1021 cggatcgggg tattcccgat agagccgggc cagggcggag gcgcactgga ccaccgcgcg
     1081 ataggtcacc ggcatggcgg ccgagagggc gatggcgaag aggtcggcgc cgggatcgag
     1141 gtcgtggcgc cgggcggcct gcagtatctc gccatatatc tccggctcgg gctgctgcca
     1201 gtcgatcgcc ggatgcagca ccgggatgtt gcgcaagggc agtttctcat aagcgaccca
     1261 gatggtgctg ccgctcagcg agagcgaata ggcaccggcg ccgatgacgg tggcgcggat
     1321 cgtctgcctg ggaagccgga ccggataggc ctgcaggcgg gggtgagtac gcaatgcatc
     1381 agccagaatg ggcccaagat ccctgaagta aaagggatca ccttgggtca attgcggatg
     1441 ataaaagcat tcacccacac cgccggagat gaagagggcg tccagtttgc cgggctcctt
     1501 cagaggcggg gtcatgagca gttcctcgag caggggcgag gagaggcctt cgataaggtc
     1561 gacgacgagg tcggccatgc gctgggctgt gcgctcgaga tcagcgcggg tcagggcgtt
     1621 gttctggggg atggagcccg ggaagaggct gcgcagcacc ctggaggccg gttcccgtac
     1681 ccgggtgacc cggccggccg gatcggtctc gatgagatgg ccgccgacgt tgagacaggc
     1741 ggtatcgatg acgcggccgt tatcaaagag cacatagttg gaggttccgc cgccgatgtc
     1801 gatgttgagg acgcgggcgg aacggctgtc ggaaaaatcg gcggctcccg atccccggcc
     1861 ggcgatgacc gactccaggt gggggcccgc tgtagcgaca acgaaatcgc cgaggcggtc
     1921 ggcgagggtc atgatggaac ggcgggcgtt gctcgccttg gaactctcgc cggtgatgat
     1981 gatcgcgccg gtctcgatct cgttgagggt tctgccggca cgggcatatt cactttggag
     2041 cagggcgaag agctgctctt catcgatgga accatcctcg tgcacggggg tcatgtgaat
     2101 ggcgctctcg tagaggatgc ggcgctcgct gaattcgtaa cgcggcacct gggtggcggg
     2161 tgcgcggttg atcagttcga gcgcggaaaa gatcagctgc agagtggtgg tgccgatatc
     2221 gatgccgaca ctgtctattt tcttggtttc actcaaatcg ccgcgctcgt aaagagggcc
     2281 ggaggggaca agcccctccg gctgttgaaa gaattacttc aggagtttcg cttccgattt
     2341 gccgactgaa atggtttcgg actggggcag aagcagaccg gtgttctcgt tgcggccctg
     2401 gaggatgaag cgcggagtct tctccgtcat ctgttccacg gcgagcttgc tggccttgcc
     2461 gatcgggccg cccttgaggc ggttgaagat cagggccagt ttcatgccct tgaactcggt
     2521 gatcatctca tagtcgccgt cgctgtcgga ggcggagaag atcggatcgc cgcgatggcc
     2581 gagcaccttt ttgatggctt ccactttgcc catgcgctgg gtctgcaccc agccggtctt
     2641 gtactgcggc agcagtatgt tatttttcac ttccagttcc atggcaatga ccttgttggg
     2701 gtcgatgttg tagccgaaat tgccaggggc gccgaaggtc tggaccacct gcttgaggct
     2761 ggcggagcag atgtaaacgt cgatgccggc tttcatcatc gattcggtca gatcctgcat
     2821 ctcgggatgg acgcgcaggc cgctcttgta ggtatagtca atcggaccgg ccttgcaggc
     2881 gaagttgcag ggggctttcc aggtcacctt ggtcagtttg tcgttaagag cgaaggtgat
     2941 cgcatctttg gccagggcct gaacctcttc gacggagtga ttggccagga agaagagcac
     3001 ccagggatag ccgtagacag cctcgatgcc ggcggtctcg caatagccgt catagaggaa
     3061 gggcatcttg gtgatgaaat ccttgaactg cggggtctcg cggatctctt caatggtcat
     3121 cttgccgacg ccgaagcccg catagttgtc atgcaggaat tcataggcgg agatgatgtc
     3181 ggcgttgatg tcggccagct tgatgttttt gtaatcggca ttgagctgct tgacaccatt
     3241 gacctcgtcc ttgaagaggg ccttgaactc ttccttgctc agcttgaact tgagattcca
     3301 gagctgatga cggaaggtcg cctcttcgca gtcaagatgg gccatggtct ggtcccagtc
     3361 gagagcgacg taggggggat tcttcttgtc gtagtacttg ccgcccacac catagtcctt
     3421 gatgaacttg gaaaggactt cgtagttgcg cggggaccag ttcattttgt tgagggcata
     3481 aacggagctg gtgcccagga aaagaacagc gatgagaagc agggctatcg ggagaaaaaa
     3541 tctcttcatg attgtgacct ccggttgatt agggatttgg gttgcctaga actccaccca
     3601 gaactgggtg acaaagcgca ggttgccttt ggattcatcg ccaaccttga agccgtcgcg
     3661 gacgacgttg aacatcagat agttctcgcc gatgatcttg tagttgaggc cggcgaggat
     3721 catgttccac tcatcattgt ccacatcgcc attgggatcg agctggccat actcgccgac
     3781 gaacatcagg ttcgggagga tgttatagtt gacatagccc atgtaggtca ggccactgac
     3841 gccatcgagt tcggggaccg cctcgacgtt cttcttgccg gtagtgactt caacaccgac
     3901 atcgagcttc ttgtcgatgg cgtactcagc gccgccgccg atccagaagt tgttgtcctt
     3961 gttgctggcc ttgttgtagt aatcggtcat ggtccaaccg aaaacggaga ggccggctac
     4021 cggtttcaac cagatgtcag cgatgacgtt tttggctttg ttgtcttctt tggcatagct
     4081 gtaggtgttc ttgttggcca cggcgaggcg ggcatagccc ttgacgccca ggctggccgg
     4141 gaggttgtag gtcgccatgg cgccggtgac ggcagagctg tagccattgt caaaagcggt
     4201 gccgctgacg taagcgaagc ggtcgatcca cgaattttcg atggtgttgt ggtagctctg
     4261 agctcccggc tgctcatagt tgccaaagag catctcgagg cccggcaaac ccaggaaatt
     4321 cttgaactga atgtaggcgt acttgatgcg ggcgttggtg ccctgattca gcagtttgcc
     4381 gttctggggg tcggtgtagt tgcggtggtt gaaatccagg gtgtagcgca tcgcgatgtt
     4441 gtccgagtat ttgtatctca tcgtgacata gccgcgatcg aagtagaagt agttggaggt
     4501 tgctttggtg tagggttcct cgaccatgta gcggtagcgc agaacggtct ggaaggtgag
     4561 tttgtcgttg agcttttcag cctgggccac gctcgaggcg aagatcagaa ctaccagcgt
     4621 caacaggatg gtaacggtga gttttttgta catactactc ctccttagat tggggtttgg
     4681 gtggattgat caagccgtgg agccaccggc cgcgatcttt cgtttgctca ttctcccttg
     4741 ggtggggcgg cttccagggc cgcctggctg cccttggggg caaggagcat ggcgaagagc
     4801 actgcggtga tgcctccgat gagtttgccg acaattactg caaagatcat gtccggcttg
     4861 tttgctgcgg tgaaaccaag atggtcgccg aaggtgaagg cggcgctgac ggcgaaggcg
     4921 acattgagga ccttaccgcg gtcatccatg ccgctcatca gcttgaacat cgggatgttg
     4981 ttggccaggg tggcgatcat gccggcggcg gcggtgctgt tcatcttgag caggcggccg
     5041 acgctgagca ggggtttttc gaaccagcgc gtgaggagat agaccatggg ataagcgccg
     5101 aggaggacga tggcgatacg gccgatgacc tcgatggcgc gcaggtcgat gccgggctga
     5161 tcaccgctga ccgggaagat cggatccatg ccggggatga tcttgaggcc ggtgagggtc
     5221 tcgaggacga tcgaggcgag gccgagtgtg atcagggcca ccagcagctt ggcgaagacc
     5281 aggaagccct tgatcatggc gttgggtcgg cgccagagtc cgagggcgat gagcagggag
     5341 acgatgacca ccgggatgag gttcttgaag atcagcccca gggtgaaaat caccgcggtg
     5401 ccgtccgctg ccttgatgtt ggagatcatg gccaccagac cgccggcgat acagccgagg
     5461 gggatggtga cgatgccggc gaggacgccg agggcgaggt gtttgcggtc ggccttgtcg
     5521 atgatgccga tgccgacggg tatcgaaaaa acgatggtcg ggcccatcat ggcgcccagg
     5581 atgaggccgg cgtacatcca ggcgggatag tcgatctggc ccgctgtggt ggccagctct
     5641 ttggcgagga aaaagccgcc catgtcattg gccagcaggg tggtggcgaa catggcgggg
     5701 ctggcgccga ggaactggta gagagggatg atgaccgggc cgagcaggcg ggccagcacc
     5761 ggggccaggc tgatcacacc caccatggcc agggcaaggg gccccatggc gttaaagccc
     5821 tcctcgaatt gggcccctgc gccgtcaaag gcgcggccca gccggccgag gcgcaccctt
     5881 ttgaaaacgc tttccgatcc cccgaactgc atcaggatgc gatccagggc gcccagcacc
     5941 atgaatgcca tcatgatgtc gatgatgatt tgattgacca gtgccatctt ttctgcttcc
     6001 ttccgggttt agctgttctc tgctgtcaga gtgcctgttg gtaaatcctg atgatatcct
     6061 ctttctgcgc cgtgcgcggg ttggtgcgca ggcagctgtc ctgcagcgcc tggtcggcga
     6121 gctggggcag atctttgtcc gtgaccccga atccggccag acccgagggc atgccgagat
     6181 cctcgatgag gtggatgacg gcggtgacag cacgctcggc ggcctcccgc cggttcaggt
     6241 tttcgaccct ctctccgagg gcggtggcga tatcgccgaa ctcttcggcc cgcaccagca
     6301 ggttgaagcg catcacatgc ggcaggatca gggcgttggc cgtgccgtgc gggatcttga
     6361 agcggccgcc cgcctgatga gacatggcgt ggcagaggcc caggccggcg ttggaaaagg
     6421 ccatgccggc catgtagcag gcgacggcca tcttgtggcg cgccagggtg ttggtgccgt
     6481 agccgacggc gatgggcaga taggtcgcaa ccaggcggat ggcgcggaag gagagcgcgc
     6541 gcgccagggt gcaggaactc tgggcgacat aggcttcgat ggcgtgggtc agcacgtcga
     6601 ggccggtggc ggcagtcacc gccggcggca ggccgatagt cagcaggggg tcgatgatcg
     6661 ccacggcggg gatcagggct ggatgttcaa agacgacctt gcggccggag gcgggatcgc
     6721 cgatgacggc gaggtcggtg acctctgaag cggtgcccgc tgtggtcggc atggcggcca
     6781 gaggaagagg cttttgcgcc agctcgctgt cgggatcgat ctcggcgaag ggaagggggt
     6841 tgcgggcgag cagggcgatc atcttggcgg catcgatagg agagccgccg ccaaccgcca
     6901 gaaccgcatc gcatcctgcg ctcttgacca tcgccgtgcc cgcgttcacc atcgcccagg
     6961 tcggttcacc cgagatctgg gcgaaaatct ccgcactcag ccccttgcgc tgcagggaac
     7021 gcaggacgac atcgaggagg ccgagctgaa ggatggtctg atcggtgacg atcagcaccc
     7081 ggctggcccc gatccgtttc atctccgcgc cggtcctggc cgcgaccccg tcacccacaa
     7141 gggccaacgg cggcacgcgg aaggtgaaat ggttggcgac ggagatctcg tccacggtct
     7201 gggcgacgat ctcttcgatg aagcgctgtt cgctgcctct ctccaaggct actgtttcct
     7261 gagattctca atgaacaatc ggatcgcttc gcgcgcggcg agcttgaccg gatcccgccc
     7321 gacagcctcg tcaaaggccc cgctgcgggc gacgacgccc tcgaaacggc cgtgggccag
     7381 cgtgacggcc ccgctgcggt caaagatggc ataatcgagc ttcgccgcca taccgcgcac
     7441 aggggcatcg ctgctccgca tcgacacatc gtcgctggcc tcctgggtga tggtcagtgc
     7501 cgcaggggcc acgatgaaat cgaacccctg gggcgccgag gtcagtctgg cgggatcgag
     7561 ggcggccgcg cgccagtaat ccttttcctg caccacggag agcaccggcg ccgcatccgc
     7621 tgctgcggcc tccgcccaat agatggtctt ccaggggccg gctgtaaagg cctgatagag
     7681 atcctgctgg atgagagcgc ggacctggtc gagatcatcg gatttgtcga agagggagat
     7741 gacggaggag gagctttcaa tatcgataag agtcagggga agcagaagga tgttcttgcc
     7801 atgaaggtca cgcttgctga aagagggatc gtccacctgc aggggtttta caaaggagtg
     7861 gcagccggcg atgatcagca gtagcagaca aaagggaggc aatatttttt tcaaggtgtt
     7921 aactcctgga ttggtttcag accaggtgca catgactgga atggttcttc tttttcaggc
     7981 cgcgggtcac cgcctgaatg agctgctggt cgtcgaaggg tttgtcgaga taccccgcgc
     8041 agccgcgttc gcgcagcccg gtgcgcagct cagtatcgcc ataagccgag atggcgatca
     8101 acgcatagta ggatctaaag tgctgcagct cctcgagcag ggccagtcca ccggaaaccg
     8161 gcatctgcag gtcgatgatg acaagctgga cgggacagcc ctggctctcc gcgagttgga
     8221 tcttcttcag ggcctcaccg agattccggg ccgagctgac cagaaaacgc gcgctttgca
     8281 ggataaaatc gatgctgtct cgcgtgtatt catcgtcatc aacgatgagt atgtgcggcg
     8341 aattatccat agcgggccct tgtttgcaca ctctacttgc aataagcagg ccaaagcggc
     8401 gcggacatct ttattgataa taaaaacagt cagatagcgc aatcaatgac aggcgggagg
     8461 cgggaaaagg ggtgccacaa aaatgccttg ccgaggtgca tgccgggcaa ggctaataaa
     8521 tacagtcgat tggatgaggt gcattgcctg gggtggaaaa aatgtggcac ctatttctcg
     8581 aggctatccg gtttccggtg caggcggttg ttgagggcgc ggcgagtgat gccaagcatc
     8641 atggcggcga ttttctggtt gccccggctg cgggcgaggg cctcctggat gagcagatcc
     8701 ccggcttctt tcatggtcgg gagcgagtcg ccgaaggcca tccgtttggc ggtggcgatg
     8761 gcggccgcgg gatccgtctc accgttttta ggcagaccat tgttgcccac cttctcgcgg
     8821 atggcctcaa gggagaggat ccccttttga tggcggctga tggtatcgag gatcatccct
     8881 tccagttcgc ggatgttgcc ggggaagagg tagttactga gcaggatgaa gagttcgggc
     8941 ggcacagccg gcaccttttt ctgcagggtg gcgcaggcct tgcggataaa gtggtcggtg
     9001 agcaggtgga tatcgtcgcg gcgctcgcgc aggggaggaa tatggatgcg atgggtttgc
     9061 aggcgataaa agagatcgcg gcggaaagct ccgctctgca ccatctcttc gatgtcgcgg
     9121 ttggtggcga caatgaagcg ggcattggag aggcggggga tatccgagcc gatcgggtaa
     9181 tagcggcctt cctggaggat gcgcagcagt ttgacctggg attcctggct cagttcgccg
     9241 atttcgtcga gaaagagggt gccgtcgccg gcctgttcga tgagaccctt gcggtcgctc
     9301 tccgcgctgg tgaagctgcc ccgcttgtga ccgaagaggg tgtcagagaa gagggtgtca
     9361 tcgatgccgg cgacattgac agcgatgaac tgcccgctgc gtccgctcgc cttgtggacc
     9421 gactgggcga tgagttcctt gccgaccccg gtctcgccgg tgatgagcac cggcaggggg
     9481 gtggtggcga ccgcctcggc gtactggaat atggagcgca tggaggcgtt ctgggtgacg
     9541 gtggaggcga aggcctccgc attcttgagg gtgttggaga ggagatactc cttgaggcgt
     9601 gagttttcat cctgcatctg gcgcagttcg gcggcgcgcc ggacggtcgt caccagccgg
     9661 ctctcgtcga cgggcttgac gaggtagtca aaagccccgc atttcatgca ttcgacggcg
     9721 gaattgacct cattgatggc ggtgaggatg atcaccggta gatgggggta ctccttgacg
     9781 atctcgggga ggagcttgga gccggagagt ccgggcatcg ccaggtcgag gagggccatc
     9841 tcgcaggggg tctcctggag cattccgagc acggcgcggc tgtcgccgca ttcgatgacg
     9901 ttgttgatgc cgttggagct gagggtcatc atcaggctga gcctgaactg ttcttcgtca
     9961 tccacaacca ggaccggcag ggcgggatag gtgatttttt tcatctctct tcgctcggtt
    10021 ttgcatttgg gccgtttttc ttctgtttgc tgcccataag cgagggtgca ctggactgtc
    10081 ctcccggcga ctgggcgatg taaagaatgg tgctcttgac cgaggtgacg aaacggtcaa
    10141 aggcgcgcgt cagctccccg atctcgtccg gccgggtgga attgaaaagg gtgttgatgt
    10201 gggcgttctt catgcgctcg gccacctcct tgatcggggt ggtgatcttg gtggcgacca
    10261 gaaagaccag gccggtcatg agcagcaggg cggccagccc gatcaggagc atcgcccggg
    10321 tgagcgagcg gacccccgcc aaggcctcct ggcgcggcac gacagcgatg aagctccagt
    10381 tgccggtctt catcggtgaa aagaagatca ggatggggtg gtgggtttca ggatcttcgg
    10441 tttcgatgaa gccgtcctcc ccgcgctgga tctgctccgc cagcttgatg aacccttcgc
    10501 cgccggcctc gcgcccgtat tcactgaggc tcttgtgacc gaccagactg cggtccttgt
    10561 gggcgatgat ggtgcccttg ttgctgacca tgaaggcgaa gccgctctcg aagagcttga
    10621 tcctggtgat catgtccgaa aggtattcga gcgagacgtc gccggtggcg acgccggtga
    10681 atttgccctc ccggaggatg ggggcggaga aggtggccat gagcacgccg cggtagagat
    10741 agggttcggt gagcagggag gtgccggtgg ccttgggaat ctggtaccag tcatcagtct
    10801 gatagttgac gaggggttcg acggcgatct tgccggtgat gcggttccag taggggagga
    10861 agcggccgtc ggcatcgctg cccggggtgc cgcggtactg actgtcgcgg ccgtcgaagg
    10921 cgttg
//
LOCUS       DAOPWU010000093        10692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6254_length_10692_cov_4.442794, whole genome shotgun sequence.
ACCESSION   DAOPWU010000093 DAOPWU010000000
VERSION     DAOPWU010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6254_length_10692_cov_4.442794"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1960)
                     /locus_tag="PKM23_06565"
     CDS             complement(<1..1960)
                     /locus_tag="PKM23_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91173.1"
                     /translation="MHKKQNLWLFAFALLLLFSLAANGQPISRPDVIWARTSDAPITL
                     DGVLSEAAWAQAESLHVIYGEEPSPIPGSGYAKEGGVDATDPTHATLKFLVQGNSLYL
                     AVIAPDSSVGGGPWAKFDAMLMNIRNAADAGRPAGSFEYFYGWVAESWADPNTANVGA
                     APGFFGWASTDRTVWDAVTVVDGITNDDAAPDKGYITEFKFDLAVRGYDATAAEGTII
                     PFNFSIYDCDWFWPLNEDKHSSNRVWVQGPWGNNSAYAVLQIHANPGITLTSALPYIG
                     PDFIVPNADTHPMPAVDGVLDEAAWAHAPGIDLRFGDDELRASYPGVVFLLSGQWQPE
                     VGGVKAPILDPADATLKWFTREDVLYLSADVRDQAVWSNEAYDQWDGIRFIINDIAER
                     DPNDHNLLRRELTVRLDAAGGALWGDYLSVVIDSMKMGAAAVTLKPGTTVNDYNDIDA
                     GYLVEIAVDLKALGYKPGLLQGNLFISATLFDGDNFANAADNYGQRVWFMREGGWNAG
                     PAWGFMDPYTTLPGGAAAENERPDAIWARTISAPLVLDGVMDEPVWEKAESVRLRYGR
                     NGYLIPGSGFGKEGGVDPEDPTDATLKFLVHGNKLYVGAQVKDASVGGGPWARFDAFL
                     MNIRDHSGEGRPAGPYEYFYGWVAESWAD"
     gene            2700..3491
                     /locus_tag="PKM23_06570"
     CDS             2700..3491
                     /locus_tag="PKM23_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY91174.1"
                     /translation="MHDPIIQIRGLRTRYGRQEIIRGIDLDIYPRETLVILGRSGCGK
                     STLLRHIVGLQRPTQGQILVKEVDMAAAAGEERDGVCKKIGMLFQGGALFNSMTVGEN
                     VALPLEEHTRLEPSTIRIMTRLKLEMVGLAGCEDLMPAQLSGGMKKRAGLARALAMDP
                     DILLCDEPSAGLDPIVAVGIDHLIRKLQQAFRMTIVVVTHELESVWLIADRIALMHAG
                     RFLFVGSQEEFKASPLPEVRQFLERQPDPNQPEGERYLASLVGER"
     gene            3516..4418
                     /locus_tag="PKM23_06575"
     CDS             3516..4418
                     /locus_tag="PKM23_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNY91175.1"
                     /translation="MEYRKSEIKAGLFIALSLILFFAILFAIKGVGAWERKESFRVRF
                     EYVGGIEPGSIVRFAGVPVGQVSSHRVLADETPPVELTLELNRGVPIRSDSYAYITSI
                     GLLGAFYIEITPGSALAEKLKSGGLIPSREVSSFAQMSGPLGGAASEATELLHRLNDL
                     LNEGNRQNLSALITNLNTLTAQNRSELTALIDNLNTLTGALNTTVTSVNTIIAANDTS
                     LQRTLQNAEQLLLQVQSLTARMNNTMGDLDRIMVQNGESYRETFDNLRMLSQNLTEFT
                     RTIKEQPWSLVRKSYPDERPLPKE"
     gene            4422..4982
                     /locus_tag="PKM23_06580"
     CDS             4422..4982
                     /locus_tag="PKM23_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNY91176.1"
                     /translation="MRRILPFVLLFLSAACGRMPRVHYYDFSLEAPARAAGSAAGVLW
                     VQPFTSLPAGDQDRMVYRDSAWEIQFDAYRRWIAPPPDLLRERLVEYLRGSSRYAAVV
                     TTPPRQEPFTVLAVQLLRFDETIAAAGRQAVTRLWFELSDERGALLRSGYIEGSAEIT
                     GADAGGLVEGMRASASWAFAQLLEKL"
     gene            complement(5103..6104)
                     /locus_tag="PKM23_06585"
     CDS             complement(5103..6104)
                     /locus_tag="PKM23_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91177.1"
                     /translation="MKKSWLLLLSLLILAGCGKKQEVVQIGAFEKFQEPFLRISFSHP
                     QGWQLQQDGPKIGFYSSLEAINKFNPYSVEGKDAARVIVNMDKMDTLKTLDEIVRSLN
                     SDLTSSGFEVSSPVAKKVSDLDGKLLHYRGALDSKNIISGDEVVAVKDSMLYTIIFEG
                     FNKMYEGSKVVLDSVLSSLHLPSFAAAAPGVDPAVPAEEMEVFENNFLKISYPANFQP
                     SPASVKDPVQFALNIYGYRQDSGVFIDVRPAQGLTLEKVVEQNAARFKETSRGSATID
                     GVPATYLNYTGGKGISSRVYFLVKNDKFYRIIYNYYQPMKGDFQPVFDKMVASIKTK"
     gene            complement(6149..7807)
                     /locus_tag="PKM23_06590"
     CDS             complement(6149..7807)
                     /locus_tag="PKM23_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY91178.1"
                     /translation="MKRFAALLSLALMLMVLPAASQDRVAEGMVLLNQGKADEAAALF
                     QKAVDLNPRNTAAMNALAKAQLQAGKPALAEATAKNALLVDDKNAETWLMLAKAQMSQ
                     GHTADAYSTLRKAMKPTKNNPELLVQLGWLHLAADSTSQAEIAFSQAKQHLPKNPDIY
                     RGLGEAYLKLGAEPVALMQFEESLKYDSIQVDLRAKMADLYLKDRRYNEAAQMYRSVL
                     NHDPENDKAALEVSRIYMLSKQYKNATSYLEKYVLRHPEDQTSWSTYMEALDKSRQYE
                     SALNAADHLLKANPNDPLALRLAGKGNYMLKQWQPAIDAYTKLAAVDTLGYEDAKRLG
                     KSYYALKNDSLALRYMEESLSKNPDQDDLYNDMGASYMRTKQWEKAAEMFQNRIKVDS
                     TYATAYVNYALCKMAVKEWEPARSALRTAVQLRPNYVYGHLYLARTLSQVDSLKAARN
                     SYETMASMADSMKSNYKNELGEAYRYITFTHLVDKNYGPALSSVTRAVEFRSDDIELQ
                     LWRAQILHALDRRDEAKAQYEKVLRMDPKNADAKKGLDILQLYN"
     gene            complement(7821..8765)
                     /locus_tag="PKM23_06595"
     CDS             complement(7821..8765)
                     /locus_tag="PKM23_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNY91179.1"
                     /translation="MWTKSKLLLLAAATVLPALTACKMQIKPKESPTSGALGVYVSDS
                     HVSLAKKEADLFASLYPDARITLYGASTRECLVHLINDSVRMVLTDRPLNKEELQIID
                     KADPKLKLDSLKVAFDALALLVNRVNDLEDLTMAGFTAIIDQEITNWQQLPASGLAGP
                     LHLVTTGKNSGAYELLKYHFLRRSEDFVPSVIAANQAEVVKYVATHAQALGIASLAAF
                     KEDTLKSLTEETDGMVRPLAFVMPDSSGAPARQKLHQGNVYLKKYPLHYPLYVYFNRK
                     SLLAAGFCSFIASSPGQKIILNSGLVPATMPIRLVQLR"
     gene            complement(8749..8973)
                     /locus_tag="PKM23_06600"
     CDS             complement(8749..8973)
                     /locus_tag="PKM23_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91180.1"
                     /translation="MNLDPGKIFAYAIAGITFCAGMALVTGMLRFQVPNQVSNIFGTV
                     LILMSLYRFLVTYLKPKPSKYRRQTNVDEE"
     gene            complement(8989..9732)
                     /locus_tag="PKM23_06605"
     CDS             complement(8989..9732)
                     /locus_tag="PKM23_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNY91181.1"
                     /translation="MTELAQKARLLYGAIELKEVKQKYMIYGLIIASVILFIGLGIYY
                     ATTLLGKEEAPVRTVRILKYSELGPPPSIQGASAAVAPSVSAAAPTAKPSVGIPVPVP
                     DADVSPEQSFASQTEMSAVSGPVGEGAGTGAGQVIEQDVQVENIDEEEAPPPDFVPFE
                     KEPQVIKRIEPVYPELARKAGLEGTVWVKLWVDKEGKVKDVVILKSASEIFNQPAIDA
                     AKQWIFTPAMMKTGPVSVWISLSFNFKLN"
     gene            complement(9756..10250)
                     /locus_tag="PKM23_06610"
     CDS             complement(9756..10250)
                     /locus_tag="PKM23_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNY91182.1"
                     /translation="MAGADVVAPKAPKGKGGKKHKKRVSVFIDMTPMVDIMMLLITFF
                     MLTTVFNTPQTMEINLPPDSDVKVEVAETSLLTIRATADGSIYVNMGMETPQRIQFNE
                     LRPLLVDRSKSNPKLVCLIKVERTGTYELMVNIMDELNLANITRFSIAPFKEIDGQLV
                     AQAI"
     gene            complement(10263..>10692)
                     /locus_tag="PKM23_06615"
     CDS             complement(10263..>10692)
                     /locus_tag="PKM23_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNY91183.1"
                     /translation="DMTPMVDIMMLLLTFFMLTTRFKPPSEAEVLLPSSHSQFKLPES
                     DVMTITVTKDDRYFLGLDSQTLRGRLFGEEYALKSGIELENKQMLADLLIQARIANPK
                     LRTVIKADKEAPFGPIEDVMNILQKVKITRFNLVTELEKI"
BASE COUNT         2072 a         2983 c         3414 g         2223 t
ORIGIN      
        1 gatcggccca gctctcagcg acccagccgt agaaatattc ataggggccg gccggccggc
       61 cttcgccgct gtggtcgcgg atgttcatca aaaaggcgtc aaagcgggcc caggggccgc
      121 cgccgaccga agcatccttg acctgggcgc cgacgtagag cttgttgccg tggacgagga
      181 acttgagggt agcatcggtg ggatcctcgg gatcgacacc cccctccttg ccaaatccgg
      241 agccggggat caggtagccg ttacggccat agcgcaggcg caccgactcg gctttctccc
      301 agaccggttc atccatgacg ccgtcaagga cgaggggagc cgagatcgtg cgggcccaga
      361 tggcatcggg acgctcgttt tcggcagcgg ccccgccggg aagggtggta tagggatcca
      421 taaaacccca ggccgggccg gcattccagc cgccttcgcg catgaaccag acgcgctggc
      481 cataattatc ggcggcgttg gcaaaattgt cgccgtcgaa aagggtggcg ctgatgaaga
      541 gattgccctg gagcaggcca ggtttgtaac cgagggcctt caaatcgacg gcgatctcaa
      601 ccagatagcc ggcatcgatg tcgttatagt cattgacggt ggtgccgggt ttcagggtga
      661 ccgcggcagc tcccattttc atgctgtcga tgaccacact cagatagtca ccccaaagcg
      721 cgccgccagc cgcatccagg cgcacggtca gctcgcggcg caggaggttg tggtcgttgg
      781 gatcgcgttc ggcgatgtcg ttgatgatga agcggatgcc gtcccactga tcataggcct
      841 cgttggacca gaccgcctga tcgcggacat cggcggagag atagagcaca tcctcgcggg
      901 tgaaccactt gagggtggca tcggccgggt cgaggatggg ggccttgaca ccgccgacct
      961 caggctgcca ctgaccggag agcagaaaaa cgacgccggg ataggaggcg cgcagttcgt
     1021 catcgccgaa acggagatcg atccccgggg cgtgcgccca ggcggcttca tccagtacgc
     1081 cgtcgaccgc aggcatgggg tgtgtgtccg cattggggac gatgaaatcg gggccgatgt
     1141 agggcagagc gctggtcagg gtgatgcccg ggttggcgtg gatctgcagc acggcgtagg
     1201 cggagttgtt gccccagggg ccctgaaccc agacgcggtt cgaactgtgt ttgtcctcgt
     1261 tcagcggcca gaaccagtcg cagtcataga tggaaaagtt gaaggggatg atcgtcccct
     1321 cagcggcggt ggcgtcatag ccgcgcacgg cgaggtcgaa tttgaattcg gtgatgtagc
     1381 ccttgtcggg ggccgcatcg tcattggtga tgccgtcgac cactgtgacc gcgtcccaga
     1441 ccgtgcggtc ggtggaggcc cagccgaaaa agccgggggc ggcgccgacg ttggcggtgt
     1501 tgggatcggc ccagctctcg gccacccagc cgtaaaaata ctcgaaggag ccggccgggc
     1561 gcccggcatc agcggcgttg cggatgttca tcagcatggc gtcaaatttg gcccaagggc
     1621 cgccaccgac cgatgagtcg ggggcgataa ccgccagata gagactgttg ccctggacca
     1681 ggaacttgag ggtggcgtgc gtcgggtcgg tggcatcgac gccgccttcc ttggcatagc
     1741 cgctgcccgg gatcgggctg ggttcctcgc cgtagatgac atgaagcgac tcggcctggg
     1801 cccaggcggc ttcactgagg actccgtcga gggtgatcgg ggcatcactg gtgcgagccc
     1861 agatcacatc ggggcgcgaa atcggttggc cgttcgctgc caggctgaaa agcagcaaca
     1921 gcgcaaaggc aaacagccag aggttctgtt tcttgtgcat gtttcctccc aatcttgatg
     1981 tcaggtttgc atcatggaga atagaaacgg actgtacagc cgcgatgcgc aaggcatcac
     2041 tctggattaa ttcacctcct tttcagggtt gagagcgttt tggggatgga tatcgcatgt
     2101 aatggaatga cttgagggga cgcttggcga aagtgcgggg ttaggcttgg gacggcgagt
     2161 ccgggggata tcggtggaga gacggggcgt atcgaggcca gggcggcaag ccggcgggct
     2221 ggccgggaga accggatccg ggcttgatgg aagaagaaca cgaggcaccc cgcttaagtt
     2281 gttcaatgaa agcgatttac atgagtaaaa cgataattaa cacgggtgaa agcctgatcg
     2341 agcgggatat catgtaaacg attacatttt acggaaaaaa aggtttgctg tcaagttaaa
     2401 tatgcaggcg ggcgaaaaaa aacgacacgg tacgcggcag aggcgccccc gtaaaagctt
     2461 aacaccgccc agaatctggg tcccacccgg ctttaatata attaaaaata gtggactacc
     2521 ggtgataagc ttgtaaagta tacggcgcga ggtgacaggt gggaacaaga tttcgagggg
     2581 tatacggcgc caggcgtcag gtgggagcca gatttcgggg gtaccctgcc agcaaaaggc
     2641 cttgaattct attttcctgc atcgtacatt tcagcatgcc gaaaccggaa gaaatgacga
     2701 tgcacgaccc catcatccag atccgcggcc tccgcacccg ctacggccgc caggagatca
     2761 tccgcgggat cgatctcgat atctaccccc gcgaaaccct ggtcattctc ggccgctccg
     2821 gctgtggcaa gagcaccctg ctgcgccata tcgtcgggct gcagaggccc acccaggggc
     2881 agatcctggt caaggaggtc gatatggccg cggcggcggg ggaggagcgc gatggtgtct
     2941 gcaaaaaaat cggcatgctt tttcaggggg gggccctctt caactcgatg acggtgggcg
     3001 agaatgtcgc cctccccctc gaggagcaca cccgcctgga gccctccacc atccgcatca
     3061 tgacccggct caagctcgag atggtcgggc ttgccggctg cgaggatctc atgcccgccc
     3121 agctttcggg ggggatgaaa aaacgggccg gtctggcccg ggccctggcg atggaccccg
     3181 acatcctgct ctgcgatgaa ccctcggccg ggctcgatcc catcgtcgcc gtcggcatcg
     3241 accacctcat ccgcaagctg cagcaggcct ttcgcatgac catcgtcgtc gtcacccacg
     3301 agctggagtc ggtctggctg atcgccgatc gcatcgccct catgcatgcg ggccgtttcc
     3361 ttttcgtcgg cagccaggag gagttcaagg cctccccgct gcctgaagtg cgccagttcc
     3421 tcgaacgcca gcccgatccc aatcagcccg agggggagcg ctatctcgcc agcctggtgg
     3481 gcgagcgctg accatcattt cacgcaagga gcactatgga gtatcgcaag agcgaaatca
     3541 aggccggact tttcattgct ctcagcctca ttcttttctt cgccatcctg ttcgcgatca
     3601 agggggtggg cgcatgggag cgcaaggagt cctttcgtgt ccgctttgaa tatgtgggcg
     3661 gcatcgagcc cggctcgata gtgcgtttcg ccggcgtgcc ggtcggccag gtcagctctc
     3721 atcgtgtcct cgccgatgag acgccgccgg tggagctgac cctggaactc aaccgcggag
     3781 tgccgatccg ctccgacagc tatgcctaca tcacctcgat cggcctgctg ggcgcctttt
     3841 acatcgagat caccccgggc tcggctctcg ccgaaaagct caaatccggc ggtctcatcc
     3901 ccagccgcga ggtctccagt ttcgcccaga tgtcggggcc gctcggcggg gctgcgtcgg
     3961 aggcgaccga gctgctccat cgcctcaacg acctgctcaa cgagggcaac cggcagaacc
     4021 tctcggccct gatcaccaat ctcaacaccc tgactgcgca gaaccgcagc gagctcaccg
     4081 ccctcatcga taatctcaac accctcaccg gcgccctcaa caccacggtg acgagtgtca
     4141 acaccatcat cgctgccaat gatacctccc tgcagcgcac cctgcagaat gccgaacagc
     4201 tgctgctcca ggtccagagc ctgacggccc gcatgaacaa cacgatgggc gatctcgatc
     4261 gcatcatggt gcagaacggg gagagctacc gcgaaacctt tgacaacctg cgcatgctca
     4321 gtcagaacct gaccgaattc acccgcacga tcaaggagca gccctggagc ctggtgcgca
     4381 agagctatcc cgacgaacgt cccctgccca aggagtgatg catgcgtaga atccttcctt
     4441 tcgtccttct gtttctcagc gcggcctgcg gccggatgcc ccgggttcac tattacgatt
     4501 tcagccttga ggccccggcg cgcgccgccg gctcggcggc gggggtgctc tgggtgcagc
     4561 ccttcacctc tctccctgcc ggcgaccagg accgcatggt ctaccgcgac agcgcctggg
     4621 agatccagtt cgacgcctac cggcgctgga tcgccccgcc ccccgatctg ctgcgcgagc
     4681 ggctggtcga gtatctgcgc gggtccagcc gttatgcggc ggtggtgacc acccctccgc
     4741 ggcaggagcc ctttaccgtt ctggcggtgc agcttttgcg ctttgatgag accatcgcgg
     4801 ctgcagggcg gcaggcggtc acccgtctct ggttcgagct gagcgacgag cgcggggcgc
     4861 tgctgcgttc gggctatatc gagggtagtg cggagatcac cggcgcagac gccggcgggc
     4921 tggtggaggg gatgcgcgcc tcggcgtcgt gggccttcgc gcagcttctg gaaaagttat
     4981 gagaatctga tattggcgcc ctgcctcccg tttgcgcctc cggcaggggc acattcccct
     5041 gcaataaaaa aagccgcccg gcctcaagcc cggcggcttt ttttgtggat cataggtgga
     5101 gattacttgg tcttgatcga ggcgaccatc ttgtcgaaaa cgggctggaa atcgcccttc
     5161 atcggctggt aatagttgta gatgatgcgg tagaacttgt cattcttgac caggaagtag
     5221 acgcgactgg agatgccctt gccgccggta taattgagat aggtggcggg gacgccgtcg
     5281 atggtggcag agccgcgcga ggtttccttg aaacgggcgg cgttctgctc gacgaccttt
     5341 tcaagggtca gaccctgggc cgggcggaca tcgatgaaga cgccgctgtc ctggcggtag
     5401 ccgtagatgt tgagcgcaaa ctgcaccgga tccttgaccg aagccgggga gggctggaag
     5461 ttggccgggt agctgatctt gagaaagttg ttttcaaaga cctccatctc ctcggcgggg
     5521 acggcgggat caacaccggg agcggcagcg gcgaagctgg gcagatgcag gctgctcagg
     5581 acgctgtcaa ggaccacctt gctgccctcg tacatcttgt tgaagccttc gaagatgatg
     5641 gtgtagagca tcgaatcctt gaccgccacc acctcatcgc cgctgatgat gttcttgctg
     5701 tcgagggcgc cgcgatagtg aagcagcttg ccatcgaggt cgctgacttt tttagcgacc
     5761 ggggaggaga cctcaaagcc ggaggaggtc aggtcgctgt tgagactgcg cacgatctcg
     5821 tcgagggttt tgagggtgtc catcttgtcc atgttgacga taacccttgc ggcgtccttg
     5881 ccctcaacgg aatagggatt gaacttgttg atggcctcga gggaggaata aaagccgatc
     5941 ttggggccat cctgctgcaa ctgccagccc tgggggtggc tgaaggagat gcgcaggaag
     6001 ggttcctgga atttttcaaa agcgccgatt tgcacgactt cctgcttttt gccgcagcct
     6061 gcgaggatca aaagactgag gagcagcagc caactcttct tcatggcagt ccctttctgt
     6121 ttgggttaag gggcggagtg aatacttatc aattataaag ctgcaggata tccaggcctt
     6181 tcttggcgtc cgcgttcttg ggatccatgc gcagcacctt ctcgtactgc gccttggcct
     6241 catcacggcg gtccagagcg tgtaatatct gggcgcgcca gagttggagc tcgatgtcat
     6301 ccgaacggaa ctcgactgct cgggtgacgg aggagagcgc gggcccgtaa ttcttgtcga
     6361 ccagatgggt gaaggtgatg tagcgatagg cttcgcccag ctcgttcttg tagttgctct
     6421 tcatcgaatc cgccatactg gccatggttt cgtaggagtt gcgtgctgcc ttgagcgaat
     6481 cgacctggga gagggtgcgt gccaggtaga gatggccata gacataattg gggcggagct
     6541 ggacggcggt gcgcagggcg ctgcgggccg gttcccactc cttgacggcc atcttgcaga
     6601 gggcgtaatt gacataggcg gtggcgtagg tggaatccac cttgatccgg ttctggaaca
     6661 tctcggcggc tttttcccac tgcttggtgc gcatatagga ggcgcccatg tcgttgtaga
     6721 gatcgtcctg atcggggttc ttggacagag actcctccat atagcgcagg gcgaggctgt
     6781 cgttcttgag ggcgtagtag ctcttgccca ggcgcttggc gtcctcatag ccgagggtgt
     6841 cgacggcggc cagtttggtg taggcatcga tcgccggctg ccactgtttg agcatgtagt
     6901 tgcccttgcc ggccaggcgg agggcaaggg ggtcattcgg gttggccttg agcagatgat
     6961 ctgcggcgtt gagcgccgat tcatactggc ggctcttgtc gagggcctcc atgtaggtcg
     7021 accaactggt ctggtcctcg gggtggcgca ggacgtattt ttccagatag ctggttgcat
     7081 tcttgtactg cttggagagc atgtagatac ggctcacctc gagggcggcc ttgtcgttct
     7141 ctgggtcgtg gttgaggacg ctgcgataca tctgcgcggc ctcattgtag cggcggtcct
     7201 tgagatagag atcggccatt ttggcgcgca gatcgacctg gatcgagtcg tacttgagcg
     7261 actcctcgaa ctgcatcagg gccaccggtt cggcgccgag cttgagatag gcttcaccga
     7321 ggccgcgata gatgtcggga ttcttgggca ggtgctgctt ggcctgggaa aaagcgattt
     7381 ccgcctggct ggtcgagtcg gccgcaaggt ggagccaacc gagctggacg agaagctcgg
     7441 gattgttctt ggtcggcttc atcgccttgc gcagggtgct gtaggcatcc gcggtgtggc
     7501 cctgggacat ctgcgccttg gcgagcataa gccaggtctc ggcgttcttg tcatccacaa
     7561 gcagggcgtt cttggcggtg gcttcggcga gggcgggctt gccggcctgc agctgggcct
     7621 tggccagggc gttcatggct gcggtgttgc gcggattgag atcgacggct ttctggaaaa
     7681 gcgctgcggc ctcatcggct ttgccctggt tgagcaggac cattccttca gcaaccctat
     7741 cctggctggc ggcgggcagt accatcagca taagcgccag cgagagcaat gcggcaaatc
     7801 gtttcatgtg ttgactactc ctatctaagc tgaaccagac gaatgggcat ggtggccggc
     7861 acgaggccgg aattcaggat gatcttctgg ccaggcgagc tggcgatgaa ggaacagaat
     7921 cctgccgcaa gcagcgactt gcgattgaaa taaacgtaga gggggtaatg gagggggtac
     7981 tttttaagat agacattacc ctggtggagt ttttggcgtg ccggcgcccc gctcgagtcg
     8041 ggcatcacaa aggcgagcgg gcgcaccatg ccgtccgtct cctcggtgag ggacttgagg
     8101 gtatcctcct tgaaagcggc cagggaggcg atgcccagag cctgggcatg cgtggccacg
     8161 tatttcacca cctccgcctg gttggcggcg atgacggagg ggacgaaatc ctcgctgcgc
     8221 ctgagaaaat gatatttgag cagctcatag gcgcccgaat tcttgccggt ggtcaccaga
     8281 tggagggggc cggcaaggcc gctggcgggc agctgctgcc agttggtgat ctcctggtcg
     8341 atgatggccg taaacccggc catggtgaga tcctccaggt cattgacccg attgaccagc
     8401 aaggccaggg catcgaaagc gaccttgagt gaatcgagct tgagcttggg atcagccttg
     8461 tcgatgatct ggagctcttc cttgttgagc ggccggtcgg tcaggaccat gcgcacgctg
     8521 tcattgatga ggtggaccag gcattccctg gtcgatgccc catagagagt gatcctggca
     8581 tcaggataga gactggcaaa gaggtcggct tcctttttgg ccagactcac atgcgaatcg
     8641 gagacgtaga cccccagggc gccgctggtg gggctctcct tgggcttgat ctgcatcttg
     8701 caggcggtca aagcagggag gaccgtcgcg gcagcgagta gcaagagttt actcttcgtc
     8761 cacattggtt tgtctcctgt actttgaggg cttgggtttg agataggtga cgagaaaacg
     8821 gtatagactc atcaggatca gcaccgtgcc gaagatgttg ctcacctgat tgggcacctg
     8881 aaagcgcagc attccggtga cgagcgccat gccggcacaa aaggtaatgc cggcaatggc
     8941 gtaggcgaag attttaccag gatcgagatt catcgtcacc gggatgcttt aattgagctt
     9001 gaagttgaac gagagcgaaa tccagaccga gaccgggccg gttttcatca tcgcgggggt
     9061 gaaaatccac tgcttggccg cgtcgatggc cggctggtta aagatctcag aagcgctctt
     9121 gaggatgacc acatccttga ccttgccctc tttgtcgacc cacagtttga cccagaccgt
     9181 gccttcgagg ccggccttgc gcgcgagttc cggatagacc ggctcgatgc gtttgatcac
     9241 ctggggttcc ttctcaaagg ggacgaagtc ggggggcgga gcctcctcct cgtcgatgtt
     9301 ttcgacctgg acgtcctgct cgatcacctg accggcgccc gttccggcgc cttcaccgac
     9361 cgggccggag accgcactca tttccgtctg ggaggcgaag gattgctcgg ggctcacatc
     9421 cgcgtcggga accgggacgg ggatgccgac gctcggcttg gcggtggggg cagccgccga
     9481 gaccgacggg gcgaccgcgg cgctggcgcc ctggatcgag gggggcggac ccagttcgct
     9541 gtacttgagg atgcgcacag tccggaccgg ggcctcttcc ttgccaagca gggtcgtggc
     9601 ataatagatg ccgagtccga tgaacagaat caccgaggcg atgatcagcc cgtagatcat
     9661 gtatttctgc ttgacttcct tgagctcaat cgccccatag aggagtcttg ccttttgcgc
     9721 aagttcagtc atgacactct ccttatgtta ggaagttaaa tcgcctgggc gacaagctga
     9781 ccgtcaattt ccttgaacgg ggcaatcgag aaccgggtga tgttggccag gttcagttcg
     9841 tccatgatat tgaccatcaa ttcataggtt ccggtacgtt ccaccttgat cagacagacc
     9901 agcttggggt tggacttgct gcggtcgacc aggaggggac gcaattcatt aaattggatc
     9961 ctttgcggtg tctccattcc catattgaca taaatggagc catcggcggt ggcgcgtatg
    10021 gtcagcagac tggtttcggc cacctcaacc ttgacatcgg agtcaggggg gaggttgatc
    10081 tccatcgtct gcggcgtatt gaagactgtg gtgagcatga agaaagtgat caagagcatc
    10141 atgatgtcca ccatcggggt catgtcaata aagacactta cgcgcttttt atgcttcttg
    10201 ccgcccttgc cttttggtgc tttgggggcg acaacatcag ctcctgccat actttaactc
    10261 cattatattt tctcgagttc cgttacaagg ttaaagcgcg tgatttttac cttctgcagg
    10321 atgttcatca catcctcgat cggcccaaag ggagcttcct tgtcagcctt gatcacggtg
    10381 cgcaatttgg gattggcgat gcgggcctga atgaggagat cggccagcat ctgcttgttc
    10441 tcgagctcga tgccggactt gagggcgtat tcttcgccga aaagccggcc gcgtagcgtc
    10501 tgcgagtcca gtccgagaaa atagcgatca tccttggtca ccgtgatggt catcacatcc
    10561 gattcgggca gcttgaactg cgagtgcgac gaagggagga gaacctcggc ctctgagggt
    10621 ggtttgaagc gtgtcgtcaa catgaagaag gtcaacaaca gcatcataat gtccaccatg
    10681 ggcgtcatgt cg
//
LOCUS       DAOPWU010000094        10630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6301_length_10630_cov_5.141371, whole genome shotgun sequence.
ACCESSION   DAOPWU010000094 DAOPWU010000000
VERSION     DAOPWU010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6301_length_10630_cov_5.141371"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /gene="deoA"
                     /locus_tag="PKM23_06620"
     CDS             complement(<1..159)
                     /gene="deoA"
                     /locus_tag="PKM23_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248048.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HNY91184.1"
                     /translation="MTVLAIIAKKRDGLALDAEEIRFLVSAYIEGTIPEYQMSAWLMA
                     VYLRGMDAR"
     gene            382..2772
                     /locus_tag="PKM23_06625"
     CDS             382..2772
                     /locus_tag="PKM23_06625"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNY91185.1"
                     /translation="MRMRILFLLLGTALLPLQAQVGWIWQNPLPQGNTLNAIQRAGGS
                     RFFAAGKFGAIIRSDDEGLTWQALSSGVTTELYALAFFNADTGLAVGARGTILRTRDG
                     GESWQRLPAPDTTTYYALYMFDYNTILAAGRGGAIIQSYNGGDLWESRASNTTQTLYT
                     LVSNGIDYTYAAGQGGALVRSLDGGGTWSPWSWAAVGTTTIRALLFPGSNIGLALGEF
                     GRVYRSQDNGGSWTLQSSLLNSPSLRAGGFIDHINGFICGSSGALFSTDNAGVTWKAL
                     ASGTSLSLQGIAFNPGGRGVLCGESGTLLSTANSGLNWSSCLDAFTTGKLRGISLADE
                     NVTVAVGDSSGKGMIFRSGDGGGTWSARLRRIGVLFEDCCFVSPDSGFVVGASSVILR
                     TSDGGLSWSQISSLPVSSSFNSVHFVTPRIGAVVGGGGAILRTNDAGATWINQTNSAY
                     GLPLQDVHFTTPSIGLAVGFLGTVLRTVNGGSTWSKINAGTVETLNGVAFADSVHAVI
                     VGAKARILRSVDRGENWSPVSVTGLPATTALEQVFFASPQVGFIVGSGGCILRSEDGG
                     ATWRLLGSPTANNLNAVCFLDLHTGSAVGDNGTVLKTVDGGLPVELTAFSARYAAAEG
                     AVHLLWRTATESRNYGFYLERRCSREWETVAFIPGNGTTAEEKHYTYIDQPGAAAGIL
                     YYRLRQMDLDGRSRVLWNIRVEVTAAVRELTLHANYPNPFNASTTLSFHLPRDGRVRL
                     ALFDAGGRECALLVDEEMTAGLHTVPWHAGSAASGLYLARLSCAGEVAVRKLLLLR"
     gene            2774..3571
                     /gene="mtgA"
                     /locus_tag="PKM23_06630"
     CDS             2774..3571
                     /gene="mtgA"
                     /locus_tag="PKM23_06630"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNY91186.1"
                     /translation="MAESDQNSPAAGEEGRGSRKWILVILLTVLVVCPLGAAAALFLT
                     LPDVEKLSEAPPAVTAMMKYREEQAEKKGRTLRRRYDWVPGRRISPYLRNAVLIAEDD
                     KFFQHQGFDWEEMRQAMEKNREKKRIVRGGSTITQQLAKNLYLKPARTPWRKLREALI
                     ARRLEKKLTKPRILELYLNVIEWGEGLYGCEAAARHYFGKSAAQLTPAEAIRLASVII
                     NPHRYSPLSDKSRRMRDRRLMLAEVMYKRHLFDEAVYEALRAEFSPP"
     gene            complement(3649..7077)
                     /locus_tag="PKM23_06635"
     CDS             complement(3649..7077)
                     /locus_tag="PKM23_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91187.1"
                     /translation="MKKTLLHLIWGVLLLAPVLVFAQAGSMLTFDGTNDFGLVIDNPL
                     LDNLTTDYTIEAWFKSTAYTNNNRLLDRNGVFALALGPNNTVQFLRPSSPELASPVNT
                     VTPGWHHVAVRVQTVGLNNTGTLFFDGLPVATLIHPSLALPASTEPVYVGNRIGFDRT
                     FSGSIDEVRLWSVALTDAAIKANRGLPLIGTEAGLLAYFKMDEGTGQVAGDATVNNLD
                     AALGSDPLLADINDPIWAPSTAPIGMNLLAPNGGTLVAGTPLNITWAVNPELTQVHIL
                     FSFDGGLKWYLLAANVPNTGTYATVVPGYPTSQLRYRISDPANGANFDDSDADITIDM
                     TGFVASSITKEGESATMNGRMYVGVDGRAFDCKFIFSAQDITGDPGIGTLNFTITTPG
                     LYVIWARALGAGSTRNSWLVQVDGGTEYLWDTTKGYKWTWDWISDRGTTGIPNVLAEL
                     DPIYFNFTAGNHTIRFRGKEHYTRLDRIVVTNNLNPDWYGSDPVEQIHITQPAEENHA
                     RIVRNTEYEIKWTSINIASKVTIEFQEHDTDLNWHLIVKDTPNDGSYIWTAPDKLLDK
                     AHIRISEEDGECPVDQTWETVQIINPPPEVFVAAPNGGEAFIFGGKTNITWTNKNFSG
                     NVNLLYSLTNGREWVGLATNILNTGTYEWTIPQVDADSCLVKVAATTGGIPMDVSDSL
                     FSIHKTLPTPAGITVTEPNGGEAWEVGSIHAVTWTVENFDGLVTLFFSSDNGANWTTL
                     AAGQPALGVYEWTIPNNVSGTCLIKIAEANTGAPVDLSDQVFAIVPAGSPGPEANYAL
                     AFDGLNDLVTVPNAPTLNISGSFTIEFWMKTDQPNQSWRRILEKGGWDEYYISFYGAT
                     GRMCGALRTTIPGGTRMNNILGPSTAVIQSNNWIHVAGTFDGTTAKMYINGVLQSTRT
                     GTAAPRNLMNDLIIGGAKHNEVYEYHYKGVLDDLRLWNIARSEADINATLFLSLSGAE
                     AGLAACYPFNEGTGQVAGDLTANNNDGRLGKLAEADESDPTWVLSDRPTSLSALAALQ
                     IIAIPAEEEAMLMELPEEFELMQNYPNPFNAGTTITYNVPNASSDQIHVKLEVYDLQG
                     RLIKTLVNGSTQPGQHQAVWDGSADNGRVTSSGVYFYRLQAGSFVETRRMIMLK"
     gene            complement(7560..8681)
                     /locus_tag="PKM23_06640"
     CDS             complement(7560..8681)
                     /locus_tag="PKM23_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNY91188.1"
                     /translation="MKSIICTLSLLISFAAAAQEPAAISSQADEIITRLGQSLVPDQR
                     LGVFHAAAEMRGDTLVVRGETTTPAACDSLLAALSAAGLAPVRCEIEELPEARLGDRC
                     VGVITVSTALLRSGPSEKEEIINQALLGETFTLLKDSHYFDLVQLSDGYVGYIDGGST
                     AVMSRSELAAWESRPKVIFWKKWGEIRSAKNDKSDPVSDIVLGTSVTLVRREGKWLRV
                     TLADGREGWLRRDEVIEAEKFARQPKPTPAQLVATARQFTGYPYLWGGRSTKGFDCSG
                     FTLTVYKLHGITLPRDANMQVFAGREVPVDSSFSALQQGDLLFFGRNRSRITHVGMYI
                     GNYRFIHSGDLVLVNSFNPAHPDFSPRRARDLQAVRRIL"
     gene            complement(8696..9304)
                     /locus_tag="PKM23_06645"
     CDS             complement(8696..9304)
                     /locus_tag="PKM23_06645"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNY91189.1"
                     /translation="MRLEISPLYPSERRLQQAIAILREGGLVIYPTDTIYGLGCDLLS
                     KRGVERIYRLKGNDKRKLLSFICPDLKEIAQYAQVSTPAYKIMKRLTPGPYTFILEAS
                     RLVPKILLEKRKTVGIRVPDHPICQRLLAELGKPIISTSATLAGQTYMQDIDQICEIF
                     QHHVDVVIDSGPGSLDPSSVIDLTGEAPVLIRAGKGDLSIFS"
     gene            complement(9327..>10630)
                     /gene="selA"
                     /locus_tag="PKM23_06650"
     CDS             complement(9327..>10630)
                     /gene="selA"
                     /locus_tag="PKM23_06650"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNY91190.1"
                     /translation="APARAALTDELTGRVRSRLDRLFTSPLAPVLNATGIILHTGLGR
                     APFAAAARARVMEIMAGYCSLELDLASGKRGERNEAVSGLICALCGAEAAVVANNNAA
                     AVLLALNTLCLNKEAIISRGQLIEIGGSFRLPDVMEKSGAIMREIGTTNKTRLRDYEK
                     AITPQTGAIVVAHTSNYRVVGFTEEPELAEIVALAHRHQLPLLHDLGGGVLVDLRRYG
                     LPYEPLVQDSLAAGADVVTFSGDKVLGGPQSGLIVGRESWIRKIHANPLMRALRCDKL
                     VFAALEATLRLYFEEGGLPASHPALAMLAEPAGQVRMRAEKVVEALAPEVKNRFAVTI
                     QASAAQAGSGALPLEKIPSHAVVLSPPDGRAEYWAARLRGANPPVLGYIQEERIWLDL
                     RTIREEETPALCSALSLALAERSTGSGLTGDDRAGDDRAGA"
BASE COUNT         1918 a         3141 c         3497 g         2074 t
ORIGIN      
        1 ccgggcgtcc atgccgcgca ggtagacggc catcaaccac gcggacatct gatactcggg
       61 gatggtcccc tcaatatagg cggagacgag aaagcggatc tcctcggcgt cgagggccaa
      121 accatcgcgt tttttcgcta taatcgctaa aaccgtcatt acatactctc gagttgcgct
      181 gccgtatcct ctccaactta accaattaat ctattgtttt caataaaaat tgacagcgga
      241 caaacttttt gtttgaattt ttgcttatcc tttgcatatt tgcactatga gacctgctgt
      301 gcatgccttt gcggcccttg atgccgagcg cagtcggaaa tctcttcgcc taagcagcgg
      361 tcagagcgaa ggagagcgat catgagaatg cgcattttgt ttttactgct ggggacagcg
      421 ctcctgcccc ttcaggccca agtcggctgg atctggcaga atccccttcc acagggcaac
      481 acccttaatg ctatccagag ggccggaggc agccggtttt tcgctgccgg caagttcggc
      541 gcgatcatcc gcagcgatga cgagggactc acctggcagg ccctttcgag cggcgtcacc
      601 accgagctgt acgcccttgc cttcttcaac gcggacaccg gcctggcggt cggcgcccgg
      661 gggaccatcc tgcgcacccg ggacgggggg gaatcgtggc aaaggctgcc tgcgcccgat
      721 acgactacct attacgccct ctacatgttt gactataata ccatcctcgc cgccggccgc
      781 ggcggtgcca tcatccagag ctacaacggc ggcgacctct gggaatcgcg cgccagcaat
      841 accacccaga ccctgtatac cctcgtctcc aacggcatcg actataccta tgccgctggc
      901 cagggcgggg cattggtgcg cagcctggac ggcggcggaa cctggtctcc ctggtcctgg
      961 gctgctgtcg ggaccacgac catccgcgcc ctcctctttc ccggcagcaa tatcggcctc
     1021 gccctcggcg aattcggccg ggtctaccgc agccaggata acggcgggag ctggaccctg
     1081 cagtcctccc tgctcaattc gccctccctg cgcgcgggcg gtttcatcga ccacatcaac
     1141 ggttttatct gcggcagcag cggcgccctc ttctccaccg acaacgcggg cgtcacctgg
     1201 aaggcccttg ccagcgggac cagcttgagc ctgcagggga tcgccttcaa ccccggcgga
     1261 cgcggcgtgc tctgcgggga gagcggcacc ctgctcagca ccgccaactc gggccttaac
     1321 tggtcaagct gcctcgacgc ctttaccacc ggcaaactgc gcggcatctc ccttgcggac
     1381 gaaaatgtca ccgtcgccgt gggcgactct tccggcaagg ggatgatctt ccggagcggt
     1441 gacggcggcg gaacctggtc ggcgcgcctc cgccgcatcg gggtgctttt cgaggactgc
     1501 tgcttcgtca gcccggacag cggattcgtg gtcggggcca gcagcgtcat cctgcgcacc
     1561 agcgacggcg gattgagctg gagccagatc tccagcctcc cggtctcgag ctcgttcaac
     1621 agcgtccact ttgtcactcc gcgcatcggc gcggtcgtcg gcgggggcgg ggcgatcctg
     1681 cgcaccaacg acgccggcgc cacctggatc aatcagacca attccgccta cggcctgccc
     1741 ctgcaggatg tccatttcac cactccttct atcggcctgg cggtcggatt tctcggcact
     1801 gtactgcgca ccgtcaacgg cggttccacc tggagcaaga tcaacgccgg gaccgtcgag
     1861 accctcaacg gcgtggcctt tgccgactct gttcatgccg tgatcgtcgg agccaaggcc
     1921 aggatcctgc gcagcgtcga ccggggagag aactggagcc cggtgagcgt caccggactg
     1981 ccagcgacga cagccctcga gcaggtcttt ttcgccagcc cccaggttgg ctttatcgtc
     2041 ggttcgggcg gctgcatcct gcgcagcgag gacggcggag ccacctggcg gctgcttggc
     2101 tcccccaccg ccaacaatct caatgcggtc tgctttctcg atctccacac cggctcggca
     2161 gtcggcgata acggcacggt gctgaagacc gttgacggcg gcctgcccgt cgagctgacc
     2221 gccttctccg cgcgctacgc agcagctgag ggagcggtac acctcctctg gcggaccgcg
     2281 accgagagcc ggaattacgg cttttacctg gaacgccgct gcagtcggga gtgggaaaca
     2341 gtcgctttca tccccggcaa cggcaccacc gccgaagaaa agcactacac ctacatcgat
     2401 cagcccggcg cagccgcggg catcctctac taccgcctgc ggcagatgga cctcgacggt
     2461 cggagccggg tgctctggaa catccgggtg gaggtgacag ctgcggtccg cgaactcacc
     2521 ctccatgcca attatcccaa tccattcaat gcctcgacca ccctgagctt ccacctgccc
     2581 agggatgggc gggtccgcct ggctctcttc gacgccggcg gccgggagtg tgccctgctt
     2641 gtggatgagg agatgactgc cgggctgcac actgtcccct ggcacgccgg cagtgctgcc
     2701 agcggcctct atctggctcg cctcagctgc gccggcgagg tggcggtacg taaactgcta
     2761 ttgctgcgct gagatggcgg agtccgacca gaacagcccg gcagcgggcg aagaggggag
     2821 aggttcccgg aaatggatcc tggtcatcct gctgacggtc ctggtggtct gccccctggg
     2881 ggccgctgca gccctctttt tgaccttgcc cgatgtggaa aagctcagcg aggctccccc
     2941 cgcagtcacg gcgatgatga aataccgcga ggagcaggct gaaaaaaagg ggcgaaccct
     3001 ccggcgccgc tatgactggg tgccgggacg gcgcatctcc ccctacctgc gcaatgcggt
     3061 cctgattgcg gaggacgaca agttctttca gcatcaggga ttcgactggg aggagatgcg
     3121 ccaggcgatg gaaaagaacc gggagaagaa gcgcatcgta cgcggaggct cgaccatcac
     3181 ccagcagctg gccaagaatc tctatctcaa gccggcgcgc accccctggc gcaagctccg
     3241 ggaagccctg atcgcccgga ggctggagaa aaagctcacc aagccgcgca tcctcgagct
     3301 ctacctcaat gtgatcgaat ggggggaggg gctttacgga tgcgaggcag cggctcgcca
     3361 ctactttggc aaatcggccg cccagctgac ccctgccgag gccatccgcc tcgcctccgt
     3421 gatcataaat ccccaccgct attcccccct cagcgacaag agccgccgca tgcgcgaccg
     3481 gcgcctgatg cttgccgagg tgatgtacaa acgccatctc tttgatgagg cggtttatga
     3541 ggccctgcgg gccgagtttt cacccccctg agcgggcagg ccataaaaaa aagcggcgca
     3601 tccggagatg cgccgctttt tttatggcgt gcggagttta tgcccgcgct acttgagcat
     3661 gatcatccgc ctggtttcga caaagctgcc tgcctgcagg cgatagaaat agacgcccga
     3721 ggaggtcacg cggccgttgt cggcgctgcc gtcccacacc gcctggtgct gaccgggctg
     3781 ggtggagccg ttgaccaggg tcttgatcag gcggccctgc aggtcataaa cctccagttt
     3841 gacgtggatc tggtcgctgg aggcattcgg tacattgtag gtgatggtgg tgccggcgtt
     3901 gaaggggttc ggatagttct gcatgagttc gaactcctcc ggcagctcca tgagcatagc
     3961 ctcttcctcg gccgggatgg cgatgatctg gagagcggcg agggcagaga gggaggtcgg
     4021 acggtcgctc agcacccagg tcgggtccga ttcatcggct tcggccagtt tgccgaggcg
     4081 gccgtcgttg ttgttggcgg tgagatcgcc ggcgacctgg ccggtaccct cattgaaggg
     4141 atagcaggcg gccagacccg cctcggcgcc gctgaggctg aggaacaggg tggcgttgat
     4201 gtcggcctcg ctgcgggcga tattccagag gcgcagatca tcgagcacgc ccttgtagtg
     4261 gtactcgtaa acctcgttat gcttggcgcc gccgatgatc agatcattca tcaggttgcg
     4321 cggcgcggct gtgcccgttc gggtcgactg cagcacgccg ttgatgtaca tcttggcggt
     4381 ggtgccatcg aaggtgccgg cgacatgaat ccagttgttg ctctggatca cggcggtgga
     4441 ggggccgagg atattgttca tgcgagtgcc gcccgggatg gtggtgcgga gggctccgca
     4501 catgcggccg gtggcgccgt agaaggagat gtagtattca tcccagccgc ccttttcaag
     4561 aatgcggcgc cagctctgat tgggctggtc ggtcttcatc cagaattcaa tggtgaagct
     4621 gccgctgatg ttcagggtcg gggcgttcgg gaccgtcacc aggtcgttga ggccgtcgaa
     4681 ggcgagggcg taattggcct ccggtccggg tgaaccagcg ggcacaatcg cgaagacctg
     4741 gtcgctgagg tcgaccgggg cgccggtatt ggcctcggcg atcttgatca aacaggtgcc
     4801 ggagacattg ttcgggatgg tccattcata gactcccagg gcaggctgtc cggcggcgag
     4861 ggtcgtccag ttggcgccgt tgtcactcga gaaaaagagg gtgaccaggc cgtcaaagtt
     4921 ctccacggtc caggtgacgg cgtggatgct gcccacttcc caggcttccc cgccgttggg
     4981 ctcggtcacc gtgatgccgg ccggggtggg cagggtcttg tgaatggaga agaggctatc
     5041 gctgacatcc atcgggatgc cgccggtcgt ggccgccacc ttgaccaggc aggaatcggc
     5101 atcaacctgg gggatggtcc actcataggt cccggtattg aggatattgg tggcgaggcc
     5161 gacccactcc ctgccattgg tgaggctgta gaggaggttg acgttgccgc taaagttctt
     5221 gttggtccag gtgatgttgg tcttgccgcc gaagatgaag gcctccccgc cattaggcgc
     5281 tgcgacgaag acctcgggcg gcggattgat aatctgcacg gtctcccagg tctggtccac
     5341 cgggcactcg ccatcctcct ccgagatcct gatgtgggcc ttgtccagga gcttgtccgg
     5401 ggcggtccag atataggagc cgtcgttggg ggtgtccttg acgatgaggt gccaattcag
     5461 gtcggtgtcg tgctcctgga attcgatcgt caccttgctg gcgatgttga tcgaggtcca
     5521 tttgatctcg tactcggtat tgcgcacgat cctcgcatgg ttctcctcgg ccggctgggt
     5581 gatatggatc tgctcgaccg gatcggagcc gtaccagtcg ggattgaggt tgttggtgac
     5641 gacgatgcgg tcaagacggg tgtaatgctc cttgccgcgg aaacggatgg tgtgattgcc
     5701 cgcggtaaag ttgaaataga tgggatccaa ctcggccagg acattgggga tgccggtcgt
     5761 gccgcggtcg ctgatccagt cccaggtcca tttgtagccc ttggtggtgt cccagaggta
     5821 ctcggtgccg ccgtcgacct ggacaagcca ggagttgcgc gtgctgccgg cgcccagggc
     5881 gcgcgcccag atgacataga ggccgggggt ggtgatggtg aaattaaggg taccgatgcc
     5941 gggatcgccg gtgatgtcct gggcggagaa gatgaacttg cagtcgaagg cgcggccgtc
     6001 gacaccgacg tacatacggc cattcatggt cgcgctttcg ccctctttgg tgatcgagct
     6061 ggcgacgaag ccggtcatgt cgatggtgat gtcagcatcg ctgtcatcaa agttggcgcc
     6121 attcgccgga tcggagatgc ggtaacgcag ctgcgaggtc ggatagccgg gcacgaccgt
     6181 ggcataggtg ccggtgttgg gcacattggc agccagcaga taccacttga ggccaccatc
     6241 gaagctgaag aggatgtgga cctgagtgag ttcggggttg accgcccagg tgatattcaa
     6301 gggtgtgccg gcgaccaggg tgccgccgtt gggggcgagc agattcatgc cgatcggggc
     6361 ggtggaaggg gcccaaatcg gatcgttgat gtcggcgagc aggggatcag agccgagggc
     6421 ggcgtcgaga ttgttgacgg tcgcatcgcc cgccacctgg cctgtgccct catccatctt
     6481 gaaataggcg agcaggccgg cttcagtgcc gatgaggggg aggccgcgat tggccttgat
     6541 cgcggcatcg gtcagggcca cggaccacag gcgcacctca tcgatggagc cgctgaaggt
     6601 gcggtcaaag ccgatgcggt tgccaacata aaccggctcg gtgctcgccg gcagcgcaag
     6661 gctgggatga atcagggtgg ctaccggcag gccgtcgaag aagagggtgc cggtattatt
     6721 gaggccgacg gtctgaacgc gcacggcgac atggtgccag ccgggggtga cggtgttgac
     6781 cggcgaggcg agttcagggc tgctcggacg gaggaactgc acagtgttgt tcggtccgag
     6841 ggcgagggcg aagacgccgt tgcggtcgag aagacggttg ttattggtgt aggccgtgga
     6901 tttgaaccag gcctcgatgg tatagtcggt ggtgagattg tcgagcaggg gattgtcgat
     6961 caccagccca aagtcgttgg tcccgtcgaa ggtcagcatg gagccagcct gcgcgaagac
     7021 caagaccggt gcgagcaaca acactcccca gatcaggtgc aataacgttt ttttcatctg
     7081 cttctccctt tatgcataag agtacatcag gccgtttgca cagcgggtgg attgtcggag
     7141 ctgtgttcgg ggcctgactg gaaattcgcc atctgccgtg acagtttttc tgtttctgct
     7201 tgcctcccag tctgaataac agcatgcctg aaaacaatta tatagatcac ctgtacttca
     7261 atacagaaaa acgaaacagc tgcgtaacaa attataacta aaatataaat aaccccaaaa
     7321 taatttacga attttagcca atattattgt gacgtgggtc accaaacggg tgccccgatc
     7381 atgttctcgt gccggccgtg aggggagtga gagaggtaca atgggatgcc aatacggggg
     7441 atgccttgct gagagcggcc gcagggtgcg gaattgtgcc gtgctatctt aaagatgaac
     7501 accacaccag atttaaaata tggttttaaa acctcaaaat caagtattga tattgttttt
     7561 tacagaattc tgcgcaccgc ctggaggtct ctggcccggc gcgggctaaa atcagggtgc
     7621 gccggattga agctgttgac gagaaccaga tctccggagt ggatgaagcg gtagttgccg
     7681 atatacatgc cgacatgggt gatcctgctg cggttgcggc cgaagaagag cagatccccc
     7741 tgctgaagtg cggagaagga agagtcgaca gggacttcgc ggccggcaaa gacctgcata
     7801 ttggcatcgc gcggcagggt gatgccgtgc agcttgtaga cggtcaaggt gaaacccgag
     7861 cagtcaaagc ccttggtgga gcgcccgccc cagaggtagg gatagccggt gaactggcgg
     7921 gcggtggcca ccagctgtgc cggagtaggt ttgggctgac gggcgaattt ttcggcctcg
     7981 atcacctcgt cgcggcggag ccagccctcg cggccgtccg ccagggtcac gcgcagccat
     8041 ttgccctccc gtctgaccag ggtgacgctg gtgccgagga cgatatccga gaccggatcg
     8101 ctcttgtcgt tctttgcact gcggatttca ccccactttt tccaaaagat caccttgggc
     8161 cggctctccc aggcggcgag ttcgctccgg ctcatcaccg ccgtggagcc gccgtcgata
     8221 tagccgacat agccgtcgct gagctggacg aggtcgaaat agtggctatc cttgagcagg
     8281 gtgaaagtct cgccaagcag ggcctggttg atgatctcct ccttttcgga ggggccgctg
     8341 cggagcaggg cggtgctgac cgtgatcacg ccgacgcagc ggtcgcccag cctggcctcg
     8401 ggcagctctt cgatctcaca gcgcacggga gccaaaccgg cagctgacag ggcggccagc
     8461 aggctgtcgc aggccgccgg ggtggtggtt tcgcccctga cgaccagggt gtcgccgcgc
     8521 atctccgcgg cggcatgaaa gactcccagc cgctggtccg ggacgaggct ctggccgagg
     8581 cgggtgatga tctcatcagc ctggctggaa atcgccgcgg gttcctgggc ggctgcggcg
     8641 aaactgatga gcaaggaaag agtgcagatg atcgatttca tgggattttt ccggtttagg
     8701 aaaagatgga gagatcaccc ttgccggcgc ggatgagcac aggcgcttcg ccggtcaggt
     8761 cgatgacact cgaggggtcg agagagcccg gcccgctgtc gatgacaaca tcgacatggt
     8821 gctgaaatat ttcacaaatc tgatcgatat cctgcatata ggtctgtccg gccagggtgg
     8881 cgctggtgct gatgatgggc ttgcccagct ctgcgagcag acgctggcag atggggtgat
     8941 ccggcacccg gatgcccacg gtcttgcgct tctccagcag gattttgggc accaggcggc
     9001 tggcctcgag gataaaggtg taggggccgg gggtgagccg tttcatgatc ttgtaggccg
     9061 gggtcgagac ctgggcgtac tgggcgatct ccttgaggtc cggacagatg aagctgagca
     9121 gcttgcgttt gtcgttgccc ttgagccggt aaatccgttc caccccccgc ttgctgagca
     9181 ggtcacagcc gaggccgtaa atggtatcgg tcgggtagat gaccagcccc ccctcgcgca
     9241 gaattgcgat ggcctgctgc aggcgccgtt cggaggggta gaggggggat atctcgagtc
     9301 gtatcatcgc atctctttca ccatgttcat gcgccggccc ggtcgtcacc ggcccggtca
     9361 tcaccggtca aaccgctgcc ggtgcttctc tccgcgaggg ccaggctgag ggcgctgcag
     9421 agcgccggag tctcttcttc gcgaatggtg cgcagatcga gccagatccg ctcttcctgg
     9481 atatatccca ggacaggcgg attggcgccg cgcaggcggg cggcccagta ctcggcccgg
     9541 ccgtcgggcg gggagaggac gacggcgtgg ctggggattt tttccagggg gagggcgccg
     9601 ctgcccgcct gcgcggcgct cgcctggatc gtgaccgcaa atcggttctt cacttcgggt
     9661 gcgagtgcct cgaccacctt ttccgcccgc atccgcacct ggcctgccgg ttccgccagc
     9721 atcgccaggg cgggatggct ggccggcaaa ccgccctcct caaagtagag ccggagggtg
     9781 gcctcgagag cggcgaagac cagcttgtcg cagcgcaggg cgcgcatgag cgggttggcg
     9841 tggatcttcc ggatccagct ctcccggccg acgatgagcc ccgactgcgg cccgccgagg
     9901 accttgtccc cgctgaaggt gaccacatcg gcccctgcgg ccaggctgtc ctgcaccagg
     9961 ggttcatagg gcagtccgta gcggcgcaga tcgaccagca cgcccccgcc gagatcgtgg
    10021 aggagcggca gctggtggcg gtgggcgagg gcgacgatct cggccagttc gggttcctcg
    10081 gtaaagccga ccacccggta attggaggtg tgggcgacga cgatggcccc ggtctgcggg
    10141 gtgatcgcct tttcatagtc cctgagccgg gttttattgg tggtgccgat ttcccgcatg
    10201 atggccccgc tcttctccat gacatcgggc aggcggaagc tgccgccgat ctcgatgagc
    10261 tggccgcgcg agatgatcgc ttccttgttc aggcagaggg tgttgagcgc cagcagcacg
    10321 gcggcggcgt tgttgttggc gacgacggcc gcctcggcgc cgcagagggc gcagatcagc
    10381 ccggagactg cctcgttgcg ctcgccgcgc ttgccgctgg ccagatcgag ttcgaggctg
    10441 caatagcccg ccatgatctc catcaccctg gcgcgggcgg ccgctgcaaa gggggcgcgc
    10501 cccagtccgg tatgcaggat aatgccggtg gcattgagca ccggggcgag cgggctggtg
    10561 aagaggcggt cgaggcggct ccggacacgg cctgtcagct catcggtcag ggccgcgcgt
    10621 gcgggggctg
//
LOCUS       DAOPWU010000095        10521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6378_length_10521_cov_5.225205, whole genome shotgun sequence.
ACCESSION   DAOPWU010000095 DAOPWU010000000
VERSION     DAOPWU010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6378_length_10521_cov_5.225205"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1833
                     /locus_tag="PKM23_06655"
     CDS             190..1833
                     /locus_tag="PKM23_06655"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNY91191.1"
                     /translation="MAHSCGNPEPPLTNRAAGTLGIVASGHEEATRAGMRILEQGGNA
                     ADAAAATLLALSVKTIGAFCIGGEVPLIYYEAASGVVTVLNGQGGAPLDSAAIAWYYA
                     NTIPGSDIRSAAVPAVVDLCVTTLKRFGTRSFSQTAAPTLKLLDAGGPEWYRDTSDGD
                     TIHTGRNWYADLAVTLRKLNEAEKRSQGEREEKLQAVADRFYRGDIADELEKWYIQEG
                     GFLRKGDLAAHVTRIETPVTLDYKGYTICKCGPWTQGPWVLQALRLLEGCDLRGMGHN
                     SADYIHTVAEAMKLALADRDEYYGDPEYVEVPLARLLSDEYTALRRPLIDSGRASLSL
                     RPGDPFGMRALRPGGGRPFPAMGGTTICAVADRWGNVAVCTPSGLGSRAGTGGATGIT
                     HGTRLVIFNTWPDHPNRIEPGKRPRTTLTPTLVLRDGRPALAISVAGGDMQDQAALQI
                     ILNVIEFGMDASEAWSARRFSTNHFIGSFGQDPPRLADLRLPDALSPEVVADLEGRGH
                     HVKVTRENPGGVALLQWEETGGVVQAAGGRSSALEGNVK"
     gene            1889..3202
                     /locus_tag="PKM23_06660"
     CDS             1889..3202
                     /locus_tag="PKM23_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY91192.1"
                     /translation="MNTEMKRRAFIKTAAVSALATSLTPRSALGNGEPARVRLGIIGV
                     GSYGTELLQMCLAMPDIDIPALCDIDVEHLARAQRLVEMSGRKKPEGYAKSETDYENM
                     VTRADLDAVVIATPWIWHTPMAVATMRAGKYCAPEVWGAASIDEVWQLVRASEESGMP
                     CMMLENHCYDRDSMAILNMVRQNLFGELVHCQCGYQHDIRNVNFGPDGELTWWGEQAL
                     KRNGDLYPTHGIGPIANCLDIDRGNRFVTLTATASKSRGMKEYIRKKFGAGHPNANRD
                     YALGDIVTTVIKCAQGESIVINYDTNSPRPYSNMYRVQGTSGLWMEDNNSIYLEGISP
                     EDTWDSFEPYQVKYEHPLWKKYLQEDPRQERGGAAWLKLRAFLECVRRKIPTPIDVYD
                     TASWIVISPLTEQSIARGGEPVEFPDFTMGRWISRKPLFGLTDAY"
     gene            3192..3926
                     /locus_tag="PKM23_06665"
     CDS             3192..3926
                     /locus_tag="PKM23_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91193.1"
                     /translation="MRIDRIYRRGCGLAAGLIFVVSAVFSGTDAVQTSGGMVRLNFRL
                     QQHREVYEKSSYGEAPQFAIWLQTHDSLDVKTLFVTRRTAQGKYVGKVECPVSLPIWI
                     GIFRRETGRADFPTPRNPFFDAVTGATPQTADIRVTALVEAGKSYDYYIEMNVAGDYN
                     PEFPRVSAQKRQDKHGNGQPSLVWRGTIEAVPGASSTPALIGRSHQYYFITEVAADMA
                     GIGSAREVFSDIAVECTAAAAPGQGL"
     gene            3934..6408
                     /gene="hrpB"
                     /locus_tag="PKM23_06670"
     CDS             3934..6408
                     /gene="hrpB"
                     /locus_tag="PKM23_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HNY91194.1"
                     /translation="MKSDPVQSAFAIERELPALRAALAESRQVILSAPPGAGKSVRVP
                     LALLDTAWLGSSRMIMLEPRRLAARRAAEFMALQLGEPVGRRVGYRIRGNSVVSAETR
                     IEVVTEGILTRMLQANPELPGVGLVIFDEFHERSIHADLGLALALDVQRHLRDELRLL
                     LMSATLDGVALAGLLPDAPVIRGEAAAWPVQTFYSRPAAGRSFEQRAADGVWRALAAG
                     EGDLLVFLPGMREIRRVEELLLERANADILICPLHGDLSARLQERALAPAPAGQRKVI
                     LSTSIAETSLTIPGVRMVLDGGLTRASRFDPRRGMSALVTLPVSRAQADQRRGRAGRT
                     APGLCFRLWSEAEHERLPEFPQPEIRTADLTSLTLDLALWGDPEGRGLSFIDAPPPAH
                     LARARELLTALGALDRRGGLTPHGRAMAALPVHPRLGHMILKGNAAGQGPTACALAAL
                     LEEGGAPRARHGLIDLNSHLEALQSAGGTGERIEAQRQRLCTAVDIPAKAHWRGEAGL
                     LVAWAYPDRIGRRRLDRPGFYLMTNGSSVALPESDPLAREEFLAIAEADAAGADGRIY
                     LAAPLSREQLRAAFAEALEKSDETVWDEREGRVRARRLTRLAAVVIEEVEVAPEPAAA
                     AAALIEGVRRTGLHCLPWDREALLFRSRAHWASRLQSGRPGFDDQSLLEGLEEWLMPH
                     LGGILRLEGLQKLRLAGLLRARLSPVQQQELDLFAPAHLTVPSGSRITIDYSTEGTPV
                     LAVKLQELFGLIETPRIGRGQVPLTIHLLSPAARPLAVTQDLRSFWLTLYPEIRKQLR
                     ARYPKHPWPEDPLSAEPTRKTLRGRR"
     gene            6590..7663
                     /locus_tag="PKM23_06675"
     CDS             6590..7663
                     /locus_tag="PKM23_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNY91195.1"
                     /translation="MKNRKCIALSLLTLLLGVSLALAQEARPWFQDAPWPRRTVNPKL
                     KTMPLIRVEGNHFVDPQGQTILFRGIAISDPDKVERQGHWSKVHFEKVKELGANVVRI
                     PVHPIAWRERTPDNYIKLLDEAVEWCTDLGMYVMIDWHTIGNLQTEVFQNPMYITTKS
                     ETFEFWRRISRHFAGHNTVAFYELFNEPTTYRGQLGPCSWAEWKKLVETMITIIRAHD
                     LETIPLVSGFDWAYDLTPLREEPIAAANIGYTTHPYPFKRSEPWPPKWEEAFGFAASQ
                     YPIVATEFGDDAHPGVPGVDPNHYGNQIIGYLEGRGISWVIWVFDPEWGGAKIKSWNY
                     ELTPGGEFFKKAMQGKLEVQARK"
     gene            7775..8662
                     /locus_tag="PKM23_06680"
     CDS             7775..8662
                     /locus_tag="PKM23_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNY91196.1"
                     /translation="MSLNTPAEIAVEVCRSGKAKTEMPLLRLIVLGILAGVYVGFGAN
                     LATKIGSAVDADSAMGIFLYGAVFSVGLMLVVIGGAELFTGNNMTCFVSALKRSSSWG
                     GLLYNWSVVFAANFAGSLLLVCIIYHSGYWQAADTASGGAALSAMGASAAAIAKAKLS
                     LTWMQAFLRGISCNWLVCLAVWLAYAARDIAGKILAIFFPIMAFVSSGFEHSVANMFF
                     IPMGIFIARGAPAAAAEGLALTPESLGGLFTIGALFKNLLPVTLGNIAGGALFTGGFY
                     WFVYLKDRPGACPEGEPPA"
     gene            complement(8673..9314)
                     /locus_tag="PKM23_06685"
     CDS             complement(8673..9314)
                     /locus_tag="PKM23_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HNY91197.1"
                     /translation="MKRSGSSEKKRMVRGMTVAFMAFILLLWAAGATAAEKDAHQPLR
                     FITRLAGTGSTDMSDPEGYEAYSSIHLDASLRWPLSQRWAAEVNLAHESREVDFFGPE
                     GEISLGSMELLPVNLLVQYHPACKRWRPYIGAGINYTLFWEKSGELNRKKLSPSFGPG
                     LQLGADLPITSHAAFNVDLKFLFVKTDWEGPDKKEVSLKIHPTVLGIGLGFEL"
     gene            complement(9364..>10521)
                     /locus_tag="PKM23_06690"
     CDS             complement(9364..>10521)
                     /locus_tag="PKM23_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY91198.1"
                     /translation="ALTAFIALGMPDGVLGVAWPSVRAGFSLPLDAVGLLIVASTTGY
                     LTSTFLSGLLVARMGIGLLLAASCALTGVGLIGYTVVPAWWMMVLLGTAAGLGAGAID
                     AGLNTYAAAHFSPGLMQCLHASYGVGATLGPVIMTAALSTMNSWRTGYIILGGFQLIL
                     AISFALTLPVWSIKTAGEDAARRLTDYKTPLGKTLRHPGAWLSMLLFFLYTGSEFSLG
                     TWTYSLLTESRGIRPEVAGLWAGSYWATFTAGRFAAGLYAGRVGVDRMVRGSLLTALF
                     GALLWWWNPAPAANLAAVGLIGLAIAPIYPALTTGTSRRVGVSHAANTIGMQMAAGSL
                     GCAIIPALIGVLARRISLEIIPFAIACIFTALLALHALATGKNPDGRVGVN"
BASE COUNT         1886 a         3447 c         3325 g         1863 t
ORIGIN      
        1 ctcgaaaaag atcgaccgaa aaaagatcca ctgcctttct ccggatctgg aggcgtccat
       61 cccggttcag gcgacggcaa aaggaagcct cctcgagttc accctccctt cactggaggt
      121 ctataatgtg gtggtgatca attgagggcg ggcgttcgat ttgtgctcgc cgggacgctc
      181 tgcctcctga tggcgcactc gtgcgggaat ccggagccgc cgctgaccaa ccgggcagcc
      241 gggacgctgg gcatcgtcgc ttccggccat gaagaggcga cccgcgccgg catgcgcatc
      301 ctggagcagg ggggcaatgc ggccgatgcc gcggcggcca cactgctggc actgagcgtc
      361 aagaccatcg gcgccttttg catcggcggc gaggtgccgc tgatttacta cgaggccgcc
      421 agcggtgtgg tgacggtgct caacggccag ggcggcgcgc cgctcgactc agccgccatc
      481 gcctggtatt acgccaacac cattccgggc agcgacatca ggtccgcagc ggtgccggcg
      541 gtggttgatc tctgcgttac cacgctgaag aggttcggaa cgcgttcttt ttcgcagaca
      601 gcagcaccca ccctgaaact cctcgatgca ggcggaccgg agtggtaccg cgacacctcc
      661 gatggtgata ccatccatac aggccgcaac tggtacgccg atctggccgt gaccctgcgc
      721 aagctgaacg aggcggaaaa gcgcagccag ggggagcggg aagagaagct ccaggcggtg
      781 gccgaccgct tttatcgcgg cgacatcgcc gacgagctgg aaaaatggta cattcaggag
      841 ggcggctttc tgcgcaaggg cgatctcgcc gcccatgtca cgcgcattga gacgccggtg
      901 accctggact acaagggcta taccatctgc aagtgcgggc cgtggaccca gggcccctgg
      961 gttctgcagg ccctgcgcct gctcgagggc tgcgatctca gggggatggg tcacaattcg
     1021 gcggattata ttcacactgt tgccgaggct atgaagctgg ccctggccga ccgcgatgag
     1081 tattatgggg atccggagta tgttgaggtg ccgcttgcga ggctgctctc ggacgaatat
     1141 acggctctgc gccgccccct gatcgacagc ggccgggcct cgctgtcgct tcgtcccgga
     1201 gaccccttcg gcatgcgcgc cctgcgtccc gggggcggac gcccctttcc cgcgatgggc
     1261 ggcaccacca tctgtgcggt ggctgaccgc tggggcaatg tggccgtctg caccccgagc
     1321 ggactgggca gccgggccgg caccggaggg gccaccggca taacccacgg cacccgcctg
     1381 gtgattttca atacctggcc ggatcacccc aaccgcatcg agccgggcaa acggccgcgc
     1441 accactctga cgcccaccct ggttttgcgc gacggccgcc cggcgctggc catcagcgtc
     1501 gccggcgggg acatgcagga ccaggctgcc ctgcagatca tcctcaacgt catcgaattc
     1561 ggcatggatg ccagtgaggc ctggtcggcg cggcgcttct ccaccaacca ttttatcggc
     1621 tcgttcggcc aggacccgcc gcggctggcg gatctgcgtc tcccggacgc gctctccccg
     1681 gaggtggtcg ccgatctcga ggggcgcggc catcacgtca aggtcacccg cgaaaatccc
     1741 gggggcgttg ccctgctgca atgggaggaa accggcgggg tggtccaggc tgcgggtggc
     1801 cgcagcagcg ccctggaagg aaatgtcaaa taatgtgagc aattttcgat tgtacctcaa
     1861 tccgatccgc cgcaaacatg gagccattat gaataccgaa atgaagcgca gggcatttat
     1921 caagaccgct gccgtctcag cgctggccac ctcgctgact ccccggagcg cactgggcaa
     1981 cggggagccc gcccgggtgc gcctgggcat catcggtgtc ggcagttacg gcaccgaact
     2041 cctgcagatg tgtctggcta tgccggatat cgatatcccc gccctctgcg atatcgacgt
     2101 cgaacatctc gcccgcgccc agcgcctggt ggagatgagc ggccgcaaga agccggaggg
     2161 gtacgcaaag agcgaaacgg actatgagaa catggtgacc cgggccgacc tcgacgcggt
     2221 ggtcatcgcc accccctgga tctggcatac gcccatggcc gtggcgacca tgcgggcggg
     2281 aaaatactgc gccccggagg tgtggggcgc cgcctccatc gatgaagtct ggcagctggt
     2341 cagggcttcc gaagagtcgg gtatgccctg catgatgctg gaaaatcatt gctatgaccg
     2401 ggacagcatg gccattctca atatggtgcg gcaaaacctc ttcggcgagc tggtgcactg
     2461 ccagtgcgga taccagcacg acatccgcaa cgtcaatttc ggccccgatg gcgaactgac
     2521 ctggtggggc gagcaggctc tgaaacggaa cggcgacctc tatccgacgc acgggatcgg
     2581 tcccatcgcc aactgcctgg acatcgaccg cggcaaccgt tttgtcaccc tcaccgcgac
     2641 cgcctccaaa tcccgcggca tgaaggaata catccggaaa aagtttggcg ccggccatcc
     2701 caacgccaac cgcgactatg ccctcggcga tattgtcacc accgtgatca aatgtgccca
     2761 gggagagtcg atcgtcatca attacgacac caattcgccg cgcccttact ccaacatgta
     2821 tcgtgtgcag ggaaccagcg gcttgtggat ggaggacaac aattcgattt acctggaggg
     2881 gatcagcccc gaggatacct gggactcatt cgagccttac caggtcaaat atgaacatcc
     2941 cctgtggaaa aagtaccttc aggaggatcc ccggcaggaa cgcgggggcg ccgcctggct
     3001 caagctccgt gcgttcctcg aatgtgtgcg gcggaaaatc cccaccccca ttgacgtcta
     3061 tgatacggct tcctggattg tgatctcgcc gctgaccgag cagtcgatcg cccggggcgg
     3121 cgagcccgtc gaattcccgg atttcaccat gggtcggtgg ataagccgca aacctctctt
     3181 cgggctgacc gatgcgtatt gatcgaatat acagaagggg atgcgggctg gcggccggcc
     3241 tcatctttgt cgtttcagcc gttttttccg gcacggacgc ggtccaaacc tccggcggga
     3301 tggtccggct caactttcgc ctgcagcagc accgcgaggt ctatgaaaaa agttcctatg
     3361 gcgaagcgcc gcagttcgct atctggctgc agacccacga cagtctggat gtgaaaactc
     3421 tctttgtcac ccggcgcacc gcccagggaa aatatgttgg caaggtggaa tgcccggttt
     3481 ccctccccat ctggatcggg atcttccgca gggaaaccgg acgcgcggat tttccgacgc
     3541 cccgtaatcc cttcttcgac gcggtgaccg gcgccacccc ccagacggcc gatatccgcg
     3601 tgacagcgct ggtcgaggcg ggaaagagct acgattatta catcgagatg aatgtggcgg
     3661 gggattacaa tcctgagttt ccgcgcgtat cagcgcagaa gcgccaggac aagcacggca
     3721 acggccagcc ctccctggtc tggcgcggta cgatcgaggc cgtgcccggt gcctcctcca
     3781 cccccgcgct gatcgggcgc agccatcagt actatttcat cactgaagtg gccgcggata
     3841 tggccggcat cggctccgcc agagaggtct tttctgacat cgcggttgag tgtacagccg
     3901 cggctgcacc tggccaggga ctctgaccgt gcgatgaaat ccgatccggt gcagtccgcc
     3961 ttcgccatcg aaagggagct tcccgccctg cgcgccgcgc tggctgagtc acgccaggtg
     4021 atcctcagcg ccccgcccgg tgcgggcaag agcgtgcgtg tgcccctggc tctgctcgac
     4081 actgcctggc tggggagcag caggatgatc atgctcgagc cgcggcggct ggcggcgcgc
     4141 cgcgccgctg agttcatggc ccttcagctc ggcgagccgg tcgggcgccg ggtgggctac
     4201 cgcatccgcg gcaacagcgt cgtgagcgca gagacccgca tcgaggtggt gaccgagggg
     4261 attctcaccc gcatgctgca ggccaatccc gaactgcccg gggtggggct ggtgattttt
     4321 gacgagtttc acgaacgcag catccacgcc gatctcggcc tcgccctggc cctcgacgtc
     4381 cagcgccatc tgcgtgatga gctgcggctg ctgctgatgt cggccaccct ggacggggtt
     4441 gccctcgccg gcctcctgcc ggatgcgccg gttatccggg gggaagccgc tgcctggccg
     4501 gtgcagactt tttacagccg tccggccgcc gggcggagtt ttgaacagcg cgccgccgac
     4561 ggggtgtggc gcgccctcgc cgccggcgaa ggcgaccttt tggtcttcct gcccggcatg
     4621 cgggaaatcc ggcgggtgga ggagcttctg ctcgagcgcg ccaatgccga catcctgatc
     4681 tgccccctgc atggagatct gtccgcccgc cttcaggaga gggctctggc gccggccccc
     4741 gctggccagc gcaaggtgat cctctcgacc agcatcgcgg agaccagcct caccatccca
     4801 ggggtgcgga tggtcctgga tggcggcctg acgcgtgcct cccgcttcga tccacggcgg
     4861 ggcatgtccg ctctggtcac cctgccggtc agccgggccc aggccgatca gcgccgcggc
     4921 cgcgccggcc gcaccgcccc cggtctctgc ttccgactct ggagcgaggc cgaacacgag
     4981 cggctgcccg aattccccca gcctgagatc cgcaccgccg acctcacttc cctcaccctc
     5041 gatctcgccc tctggggcga cccggagggg cgcggcctct ccttcatcga tgccccgccc
     5101 cccgcacacc ttgcccgcgc gcgcgaactg ctcaccgccc tcggggctct cgaccgccgc
     5161 ggcgggctca cccctcacgg acgagccatg gccgccctgc cggtccaccc ccgcctcggt
     5221 catatgatcc tcaagggcaa cgcggccggc cagggtccga ccgcctgcgc cctggccgcc
     5281 ctgctggaag aggggggagc cccccgcgcc cggcacgggc tgatcgacct caacagccat
     5341 ctcgaggccc tccagtcggc gggcggcacc ggcgagcgca tcgaggctca gcggcagcgc
     5401 ctttgcactg ccgtggacat acccgccaag gcccattggc gcggagaggc cggcctcctc
     5461 gtcgcctggg cctaccctga ccgcatcggc cgcaggcggc ttgaccgccc gggcttttac
     5521 ctgatgacca acggctcatc tgtcgcactg cccgagagcg atcccctcgc ccgcgaggag
     5581 tttctcgcca tcgccgaggc cgacgccgcc ggggcggacg gccgcatcta tctggccgca
     5641 cccctgagcc gggagcagct gcgggcggcc tttgcggagg ccctggagaa aagcgatgag
     5701 acggtctggg atgaacgcga gggaagggtg cgggcgcggc ggctgacccg gctggccgcc
     5761 gtggtgatcg aggaggtgga ggtggcgccc gaaccggcag cagccgctgc cgccctgatc
     5821 gagggcgtcc gccgcaccgg actgcactgc ctgccctggg acagggaggc gcttcttttt
     5881 cgcagccgcg cgcactgggc gagccgcttg cagagcgggc ggcccggctt cgacgatcaa
     5941 agcctgctgg aggggctgga ggagtggctg atgccccatc tcggcggcat cctcagactg
     6001 gagggactgc aaaaactgcg gctggccggc ctcctgcgcg cgcggctcag cccggtccag
     6061 cagcaggaac tcgacctgtt cgcccccgcc catctcaccg tcccgagcgg ttcgcgcatc
     6121 accatcgatt attccacgga gggaaccccg gtgctcgccg tcaagctgca ggagctgttc
     6181 ggcttgatcg aaaccccgcg catcggccgc ggccaggttc ccctcaccat ccacctgctc
     6241 tcaccggccg cccggccgtt ggcggtcacc caggatctgc gcagcttctg gctgacgctt
     6301 tatcctgaaa ttcgcaagca gctgcgcgcc cgctacccca aacacccctg gccggaagat
     6361 cccctctccg ccgagcccac ccgcaagacc ctgcgcggca ggcgctgacc cggctctcca
     6421 tacccccgct tcactggtcg cttcgagaaa tttcgctttc gcctttagag gatcaggcaa
     6481 tccagccggg gggctaaatt aaaacacggc tccattaaaa accgcttgaa ttctattccc
     6541 cgattggcta attttcagct catcaaccta aaatcaggga gtgccgcaca tgaaaaatcg
     6601 caaatgcatc gccctctctc tcctgacgct gctccttggc gtctctctag ccctcgcaca
     6661 ggaggccagg ccgtggttcc aggatgcccc ctggccgagg agaacggtca atcccaaact
     6721 gaagaccatg ccgctcatcc gggtcgaggg caaccatttc gtcgaccccc agggccagac
     6781 catcctcttt cgcggcatcg ccatctccga tccggacaag gtggagcgcc aggggcactg
     6841 gagcaaggtc cattttgaaa aggtcaagga actgggcgcc aatgtcgtcc gcatcccggt
     6901 gcacccgatc gcctggcgcg agcgcacgcc ggataactac atcaagctgc tcgacgaggc
     6961 ggtcgaatgg tgcaccgacc tggggatgta tgtgatgatc gactggcaca ccatcggcaa
     7021 cctgcaaacc gaggtttttc agaacccgat gtacatcacc acgaaatcgg agacctttga
     7081 attctggcgc cggatctccc gccactttgc gggccataac accgtcgcct tttacgagct
     7141 cttcaacgag cccaccacct accgcggcca gctcggcccc tgctcctggg cggaatggaa
     7201 aaagctggtc gagaccatga tcaccatcat ccgcgcccac gacctcgaga ccatccccct
     7261 ggtcagcggc tttgactggg cctacgacct gacccccttg cgcgaggaac ccatcgccgc
     7321 cgcgaacatc ggctatacca cccatcccta tcccttcaag cgctccgagc cctggccgcc
     7381 gaaatgggaa gaggccttcg gcttcgccgc cagccagtac cccatcgtcg cgaccgaatt
     7441 cggcgatgac gcccatccgg gtgtgcccgg ggtggatccc aaccactacg gcaatcagat
     7501 catcggctat ctggagggca gggggatcag ctgggtgatc tgggttttcg atcccgaatg
     7561 gggcggggcc aagatcaaga gctggaatta tgagctcacc cccggcgggg agttcttcaa
     7621 aaaggcgatg cagggcaagc tcgaggttca ggcccgcaag tgaccacccc ggcggcgcgg
     7681 tcggcagatt ccggcagcga aggagaacaa cgggacgccg gggccgctgc ggatgtgcaa
     7741 gccttatgtc ggaacctgaa gaaggaaagc gatgatgagt ttaaatactc ctgcggagat
     7801 tgctgtggag gtatgccggt ccggcaaggc caagacggag atgcccttgc tgaggctgat
     7861 tgttttgggg atattggccg gggtctatgt gggatttggc gccaacctgg cgaccaaaat
     7921 cggcagcgcg gtggatgcgg actcggccat ggggatcttt ttgtacggtg ccgtcttttc
     7981 cgtcggactg atgctggtcg tcatcggcgg cgccgagctc tttaccggca acaacatgac
     8041 ctgttttgtc tcggctctga aaagaagctc ctcctgggga ggtttgctct acaactggag
     8101 cgtggtcttt gccgccaatt ttgccggttc gctgcttctg gtctgcatca tctaccacag
     8161 cggttattgg caggcggctg atacagccag cgggggcgcg gcgctcagcg ccatgggggc
     8221 cagcgccgcc gccattgcca aggccaagtt gagcctgacc tggatgcagg ctttcctgcg
     8281 cggcatcagc tgcaactggc tggtatgtct ggcggtctgg ctggcctatg ccgcccggga
     8341 tatcgccggc aagatcctgg ccatcttttt cccgattatg gccttcgtat ccagcggttt
     8401 cgaacacagt gtcgccaaca tgttcttcat cccgatgggg atctttatcg cccggggggc
     8461 gccagcggcc gccgctgaag ggctcgccct gactccggag tctttgggcg gtctcttcac
     8521 catcggcgcc ttgtttaaaa atttgctccc ggtgaccctg ggcaacatcg ccggcggggc
     8581 tcttttcacc ggcgggttct actggtttgt ctacctgaag gatcgtccgg gagcctgccc
     8641 tgagggagag ccccccgctt aggagggccg gtttagagtt cgaaccccag tccgatcccg
     8701 agcacggtcg gatgtatctt cagggagacc tcttttttgt ccggcccctc ccagtcggtc
     8761 ttgacaaaaa ggaatttcag atcgacattg aacgccgcat gggaggtgat gggcaggtcg
     8821 gcgcccagct gcagcccggg gccaaagctc ggtgagagct ttttccggtt cagttcgccg
     8881 ctcttttccc aaaagagggt atagttgatg ccggcgccga tatagggacg ccagcgtttg
     8941 caggcggggt ggtactggac gagcagattg accggcagca gctccatcga gccgaggctg
     9001 atctccccct ccggcccgaa aaagtcgact tccctcgatt cgtgggcgag attcacctct
     9061 gcggcccatc gctgcgagag cggccagcgc agactggcgt cgagatggat cgagctgtag
     9121 gcctcatagc cctcgggatc ggacatatcc gtggatccgg tacccgccag gcgcgtgatg
     9181 aaccgcagcg gctggtgcgc gtccttctct gccgccgtcg ctcctgctgc ccacagcagc
     9241 aggatgaagg ccatgaaggc cactgtcatt cccctgacca ttctcttctt ttccgacgaa
     9301 cctgagcgtt tcatttttat ctcctttcct ttaaaaaagc tatcaggtgc tctctccagt
     9361 cattcaattt actccgaccc ggccatccgg gtttttgccg gtggccaggg catgcagggc
     9421 gagcagagcc gtgaagatgc aggcgatggc gaaggggatg atctccagcg agatgcggcg
     9481 ggcgagaacg ccgatcaggg cgggtataat ggcgcacccc agcgagcccg ccgccatctg
     9541 catgccgatg gtattggcgg catggctcac ccctacgcgc cggctggtcc ctgtggtcag
     9601 ggccggatag atgggggcaa tggccaggcc gatcagtcca actgcagcga ggttcgcggc
     9661 cggggcggga ttccaccacc acagcagggc gccgaacagg gccgtgagca ggctgccgcg
     9721 caccatccgg tcgacgccca cccgacctgc gtatagtccg gcggcgaacc ttccggccgt
     9781 gaaagtggcc cagtagctgc cagcccacaa gccggccacc tccggccgga tcccgcgcga
     9841 ttcggtgagc agggagtagg tccaggtccc cagcgagaat tcgcttccgg tatagagaaa
     9901 gaagaggagc atgctcagcc aggcgccggg atggcgcagg gtcttgccca ggggcgtttt
     9961 gtaatcggtc aggcgccggg cagcgtcctc tcccgctgtt ttgatgctcc atacgggcag
    10021 ggtaagggcg aaggagatgg caagaatgag ctggaagccg cccaggatga tgtagcccgt
    10081 gcgccaagaa ttcatcgtcg acagcgcggc ggtcatgatg accggcccga gggttgcccc
    10141 cactccgtag ctggcgtgaa ggcactgcat caatccgggg ctgaaatggg cggcggcgta
    10201 ggtgttgagg ccggcatcga tcgcgccggc gcccaggccg gccgctgtgc cgagcaggac
    10261 catcatccac caggcgggga ccacggtgta accgatcagc cccaccccgg tgagggcgca
    10321 gctggccgcc agcaggaggc cgatgcccat gcgggcgacc aggaggccgc tcaggaatgt
    10381 ggaggtcaga taaccggtcg tcgaagctac aatcaggagc ccgaccgcat cgagagggag
    10441 ggaaaaaccg gcgcggaccg agggccatgc cacccccaga actccgtcgg gcatgcccag
    10501 ggcgatgaaa gcggtgagag c
//
LOCUS       DAOPWU010000096        10472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6403_length_10472_cov_4.547854, whole genome shotgun sequence.
ACCESSION   DAOPWU010000096 DAOPWU010000000
VERSION     DAOPWU010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6403_length_10472_cov_4.547854"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PKM23_06695"
     CDS             <1..230
                     /locus_tag="PKM23_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNY91199.1"
                     /translation="GRVLARANLWPYAALLAGLMLIFNGYLTARPVVLYDPAYQLDWR
                     VGTIPIEDFLYGYSLLLGGTSLFEKFRRPHG"
     gene            223..1695
                     /gene="crtI"
                     /locus_tag="PKM23_06700"
     CDS             223..1695
                     /gene="crtI"
                     /locus_tag="PKM23_06700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNY91200.1"
                     /translation="MARQVAVIGGGLAGLSGAIELARRGFSVDLFEQTGRLGGKMNEV
                     RREGFRFDTGPSLLTMPFVLEKLFADAGFRRREFLDLVPIEPMCRYFFADGSRLDASS
                     DAAAMETAITMLSERDRGRFARFMAYSRRIYDLTAEVFLFTPIHEWKRVLKSRHLSTL
                     GALPAIDPLRTVHRGVKRFFRDDRLVQLFDRYATYNGSNPYQAPATLNIIPYVEYGFG
                     GFYVRGGMYRLVEALQRLATILGVRIHTGMPVEEIVTEKGRVAGVRVDGTLLRTERVL
                     CSADVVTAFNRLLPGFAKERRRLNRLEPSLSGMIFLWGVKKSHPRLAHHNIFFSADYS
                     HEFSQIFDGLRAPDDPTVYVAITSRADAEDAPEGMENWFVLVNMPYLRPGAAESDAAS
                     VRQTVLARLRQQEIDVNGAIACEEVITPRDFLERYGSNAGSIYGISSNSRSMAFRRPA
                     NRSRAVPGLFLAGGACHPGGGIPLVLLSGRMAAELIAEME"
     gene            1771..3264
                     /gene="crtI"
                     /locus_tag="PKM23_06705"
     CDS             1771..3264
                     /gene="crtI"
                     /locus_tag="PKM23_06705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNY91201.1"
                     /translation="MPSAKRIVVIGAGFGGLAIANRLQARGFQVTLLEKNPKVGGHGY
                     PLEIDGYHFDMGPSLITEPALLEEIFTMAGKRLADYVDILPLDPYYRIYFHDNTFIDY
                     TGDGDRMRSQMAQFNPRDAARYDNFIEASRGLYQAVIVDGLGTRPFMDWRSMLSFAPR
                     ALRLKAVLPSYHFTSLYFRDFRHRFIYSFHPLFIGGNPFRAPAVYQMIPYLEKIHGVL
                     YSKGGMYSLVRALEKLFLEQGGTLHTSRPAQEILVRDGRATGVRTPEGEVPADIVISN
                     ADFIHTYRDLIRPEHRKKWSDRKLERVDYSMSAFLIYLGLNKRFPQLLHHTLVLSSRY
                     RGLIRDIFDRKVVPEDFSLYLHTPTRTDDTMAPPGCESLYILAPVANLKGGQNWSELA
                     EPFAQRILDHLEHHLGLTGLQESIAVKRLFTPEDFQRKQNAVYGSAWGVEPRLTQTAI
                     LRPHNRSEDVRGLYLVGASTHPGAGLPGVVLTAATTDYVIQRDLERG"
     gene            3269..4144
                     /locus_tag="PKM23_06710"
     CDS             3269..4144
                     /locus_tag="PKM23_06710"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNY91202.1"
                     /translation="MPQFWRDVQLAPSYTYARAITRSYARSFFFASRFLPEEKRWATW
                     ALYGFCRYADNLIDLQRTRGPEELLSELGCLSKEISLAYRTGESEHPVIKSFIDTARR
                     YQIPERYPLELIKGVEMDATFKGYKSYDDLHLFAWRVAGVVGLMMTCLLGYRDESAFP
                     YAEKLGIAMQLTNILRDIQEDKQLGRIYLPADELHRFGVAPEDILSERMTPQLRALME
                     FQVQRAHVCYAEAEPGIALLDRESRFSIYTASRLYQEILKKIELAGYNPFAGRAFVPT
                     KHKLAILLQEYARRR"
     gene            4173..4901
                     /locus_tag="PKM23_06715"
     CDS             4173..4901
                     /locus_tag="PKM23_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNY91203.1"
                     /translation="MITARHSKAAQALFHLYIMPAMQRQFHSVHLLGDEPAWPAHTPL
                     IILPNHSTWWDGFVIYLLNIRLWRRQPWVMMLEEQLRKRPFFRRLGVFSIDPATPGGV
                     RRSLRYTRELLSGEEAGGRMLCLFPQGELLPWDHRPLGYKPGLIWLLQRLESPVCLVQ
                     LGIRGEFLQQQRPQLFLEFSPPLEWSGGENTLREWEQAHAALLERLSGRIAAGEEGRI
                     LLQGRRSVDARWQYWRGLLRREVL"
     gene            4898..>5994
                     /locus_tag="PKM23_06720"
     CDS             4898..>5994
                     /locus_tag="PKM23_06720"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY91204.1"
                     /translation="MNGLLIALYALLTMIFLVSLFNTLTAPMVRKGPQPRRTPVVSLL
                     VPARDEEHTIARCLDSLCAQEYPELEIIILDDHSTDRTAAIVGDHAAADPRLRLVQGE
                     PLPEGWTGKNWACHQLSREASGEILLFTDADNFHAPFAVARTVGWIEKLDLDLFSAFP
                     QQITLSWAEKLVVPIFDLFVYSLLPLWLTWYSRFPSLSAANGQWLAFTRRGYEKSGGH
                     AAVRGHIVEDTALSRRAKQLGLKTITASGRGAVYGRMYTSPREVWLGFAKNAFGLMNF
                     RALPYFLFMGFLFFIFVLPYLLLLVPALRIWALPAVGINILLRLMVALKFGQPLVYSV
                     ILHPLSILATITVGLASFYYFLKGNIAWKGRA"
     assembly_gap    5995..6094
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6095..6635
                     /locus_tag="PKM23_06725"
     CDS             <6095..6635
                     /locus_tag="PKM23_06725"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNY91205.1"
                     /translation="LRWWGFLTGVVLSAFGLEWAGVTTGRIFGHYGYGDKLQPQLFGV
                     PLAIGFAWLAMVLTSAGVADWLPDAWRHLFRGRQGNESVSGADRPASDHGLLRILLIA
                     LLMLLFDWIMEPAAVRLGYWSWRGGIIPLQNYLAWFVFALVFAAAAWRLGFLQRGAPA
                     LSRHAWLAQILYFLLVRLG"
     gene            6652..7377
                     /locus_tag="PKM23_06730"
     CDS             6652..7377
                     /locus_tag="PKM23_06730"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNY91206.1"
                     /translation="MASLDGSGKWRGLFIAALIYLGWLGHLIFWLSAELDRSLLPWIA
                     PALLVQTWLYVGLFITAHDAMHGAVVPGREGLNRRVGQAAVFSYALFSFKKLLQRHGL
                     HHSFPASEKDPDFHDGVHTGPLRWYLHFMLHYLTIPQLVGMALLFNLLQHGAGVPLAN
                     LLLFWVAPALLSTFQLFYFGTYLPHRRPQEGYDNMHRALSSDYPVWLSLLTCFHFGYH
                     LEHHVQPTVPWWRLPRIHLPGER"
     gene            complement(7400..8257)
                     /locus_tag="PKM23_06735"
     CDS             complement(7400..8257)
                     /locus_tag="PKM23_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNY91207.1"
                     /translation="MKKAGLIAMFLITLCLICACAKGPDQPLRCMSFNIRYDTPDDGG
                     NTWPLRQEMVARTFSFHRVDVAGLQEALNRQVLVLAGLLPEYDWIGVGRDDGREEGEY
                     VPIFYRRDRLRLIRQGTFWLSPSPDSAGSRGWDAALPRIATWAEFRDRATGKDFFYFN
                     THFDHIGVRARQESSALILRKISALAGGRPVILSGDFNSTRQEGAYLELVREPEGMKD
                     SEMLSVNGHYGGSQSFTGFNDALQPNYLIDFIFVKGIRRAANHGLVAERWDGRFVSDH
                     YPVIVDIYL"
     gene            complement(8349..>10472)
                     /locus_tag="PKM23_06740"
     CDS             complement(8349..>10472)
                     /locus_tag="PKM23_06740"
                     /inference="COORDINATES: protein motif:HMM:NF037701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91208.1"
                     /translation="PANDPNIQYLGRWDFSDPLAPSHSWPGVCIQARFEGTSIGVRMT
                     DNFCYYNIIIDGIPHAVFHPTAAALTTYMLATGLSDGLHTIRIEKRNETTWAKFSFHG
                     LVIDDGKILHPPEQALQRRIEFVGDSYTSASGNEYDKPDKPAEDAPLTNISEGFGPIT
                     AHHFGAEYMMTSRSGYGMVMDWQGSRSGSLPAIFGQTHIHTATPPWDFSTWAPHLVVI
                     GLGLNDYSGMGGWNGAISESNRALYKSEYHKFIATLRDLYPGVKILAVAPHVEWLQLV
                     ISEIVIEENAAGNGDVYYAFYPYYQGGYVYDGHPSVATHHKIAEELIKAINGFDAWSP
                     YDDTRPPVFTRFPDSPQTSYSREITLEFETDTYATVRYSSQDESWGEMEQTCTTTGQR
                     KHAVTLSGEHGRNYLYYFRAADLRGNAMAASFAVAFEIDTTKILLNWQDPGYDDGGWQ
                     SGAAPVGYGTSSGLKTPCGTVQTAYFRKSITVANAAALTGLGLLVKGRDGAVVYLNGH
                     EIERINLPADEEISAATPAATALAANLSKMVVINAANGLQFLRTGENQIAVEMHAFDY
                     ARGIAFDAQLIDNTNTIIFKQGSEWKFADGGEEPEKQLRDKLAGISGADDQELPQGFL
                     LQQNYPNPFNLSTTIGFELPVGQEISLRIYDVKGAEVATLAGGALPAGLHQVRWIADK
                     AASGLYFIRLKSPDRQVLRKALLLK"
BASE COUNT         1760 a         3398 c         3127 g         2087 t
ORIGIN      
        1 gcggccgggt gctggcccgc gccaacctct ggccctacgc cgcccttctc gccgggctga
       61 tgctgatttt caatggctat ctgaccgccc ggccggtggt gctctacgat ccggcctacc
      121 aactcgactg gcgggtcggc accattccga tcgaggattt cctctacggc tacagcctcc
      181 tcctgggcgg cacgtcactc tttgaaaaat tcaggagacc ccatggctag acaggtggcc
      241 gtcatcggag ggggattggc gggattgagc ggcgccattg aactggcgcg gcggggcttc
      301 agcgtcgacc ttttcgagca gaccggccgc ctcggcggca agatgaatga ggtgcggcgg
      361 gagggcttcc gcttcgacac cgggccctca ctgctcacca tgccctttgt gctcgaaaag
      421 ctctttgccg atgccggctt ccggcgcagg gagttcctcg atctggtccc gatcgagccg
      481 atgtgccgct attttttcgc agacggcagc cgtctcgacg cgagcagcga tgccgcggcc
      541 atggagacgg ccatcaccat gctctcggag cgggaccggg gccgcttcgc tcgctttatg
      601 gcttacagcc gccgcatcta cgatctcacc gccgaggtct ttctcttcac cccgatccac
      661 gaatggaaaa gagtgctcaa gagccgccat ctctccaccc tgggcgccct tcccgccatt
      721 gaccccctgc gcacggtcca ccgcggggta aaacgttttt tccgcgacga tcgcctcgtc
      781 cagctttttg accgctacgc cacctataat ggctccaatc cctaccaggc gccggcgacc
      841 ctgaatatca tcccctacgt tgaatacggg ttcggcggct tttacgtccg cggcgggatg
      901 taccgtctgg tcgaggccct gcagcggctg gctactatcc tcggggtgcg catccacacc
      961 gggatgccgg tggaggagat tgtgaccgaa aaagggcggg tcgccggcgt gcgggtggac
     1021 ggtaccctgc tgcgcaccga gcgcgtgctc tgcagcgccg atgtcgtcac cgccttcaac
     1081 cggctgctgc ccggattcgc caaagagcgc cgccggctca accgcctcga accctcgctt
     1141 tcgggcatga ttttcctctg gggggtgaaa aagagccatc ccaggctggc ccaccacaac
     1201 atctttttct ccgccgatta cagccatgaa ttcagccaga ttttcgacgg cctgcgggcg
     1261 ccggacgatc ccaccgtcta tgtcgccatc accagccgcg ccgatgcgga ggatgccccg
     1321 gaagggatgg agaactggtt tgtgctggtc aatatgccct acctgcgccc gggggcggct
     1381 gaatccgatg ccgccagtgt gcgccaaacc gtacttgccc ggctgcggca gcaggagatc
     1441 gatgtcaacg gggcgatcgc ctgcgaggag gtcatcaccc cgcgcgactt tctcgagcgc
     1501 tatggcagca acgccggcag catctacggc atctcgagca actcgcgctc gatggccttt
     1561 cgccgcccgg ccaaccgcag ccgcgccgtg ccggggctgt tcctcgccgg cggcgcctgc
     1621 catcccggcg gcggcatccc cctggtgctg ctttcgggcc ggatggcggc cgaactgatc
     1681 gcggagatgg aataaaaaaa acgcgggacg gtgttttccg tacgaatgaa aaggctcgca
     1741 tcaccatgga ccggagagga attttactca atgccatcag ctaaaaggat cgtcgtcatc
     1801 ggagccggtt tcggcggact ggccatcgcc aatcgcctgc aggcgcgggg atttcaggtc
     1861 accctgcttg aaaagaatcc caaggtgggc ggccatggct atcccctcga gatcgacggc
     1921 taccacttcg acatgggccc atcactcatc accgaaccgg ccctgctcga ggaaattttc
     1981 accatggccg gcaaaaggct ggccgattat gtcgacatcc ttcccctcga tccctattat
     2041 cgcatctatt ttcatgacaa caccttcatc gattataccg gcgacggcga caggatgcgc
     2101 agccagatgg cgcagttcaa tccccgcgac gccgcccgct acgacaactt tatcgaggcc
     2161 tcgcgcgggc tttaccaggc cgtcatcgtc gatggcctgg gcacccgtcc cttcatggac
     2221 tggcgcagca tgctttcctt cgccccgcgc gccctgcgcc tcaaggctgt gctgccctcc
     2281 tatcatttca ccagcctcta cttcagggac tttcgccacc gcttcatcta ctctttccat
     2341 cccctcttca tcggcggcaa tcccttccgg gccccagcgg tctatcagat gatcccctat
     2401 ctcgaaaaga tccacggcgt gctctacagc aaggggggca tgtacagcct ggtccgcgcc
     2461 ctggaaaaac tcttcctcga acagggggga acccttcata ccagccggcc ggcgcaggag
     2521 atcctggtgc gcgacggccg cgccaccggc gtgcgcaccc cagagggtga agtgccggcc
     2581 gatatcgtca tctccaacgc tgatttcatc cacacctacc gtgacctgat caggccggaa
     2641 caccgcaaaa agtggagcga ccgcaagctg gagcgcgtgg actattcgat gagcgctttt
     2701 ctgatctatc tcggcctcaa caaacgcttt ccccagctcc tgcatcatac cctggtgctt
     2761 tcgtcccgtt accgcggcct catccgcgac atctttgacc gcaaggtggt gccggaggat
     2821 ttctccctct atttgcacac tcccacccgc acggacgata ccatggcccc tccgggctgc
     2881 gagagtctct acatcctcgc ccctgtcgcc aatctcaagg gtggccagaa ctggagcgaa
     2941 ctcgcggagc ccttcgcgca gcggattctc gaccatctcg agcaccatct cggactgacc
     3001 ggcctgcagg aaagcatcgc ggtcaaaagg ctcttcaccc cggaggattt tcagcgcaag
     3061 cagaatgccg tctacggcag cgcctggggc gtggagccga ggctgaccca gactgcgatc
     3121 ctgcggccgc acaaccgcag cgaggatgtg cgcgggctct atctggttgg cgccagcacc
     3181 caccccggcg ccggcctgcc gggggtggtc ctgaccgcag ccacgaccga ttatgtgatc
     3241 cagcgcgatc tggagagggg ctgatcttat gccgcagttc tggcgggatg tccagctggc
     3301 cccttcctac acctacgccc gggcgatcac ccggagctac gcgcgcagct ttttcttcgc
     3361 ctcgcgcttc ctccccgagg agaagcgctg ggccacctgg gccctctacg gattctgccg
     3421 ctacgccgac aacctcatcg acctgcagcg gacgcggggc cccgaagaac tgctctccga
     3481 gctcggctgc ctctcgaagg agatctccct ggcctatcgc acaggtgagt ccgagcaccc
     3541 ggtcatcaaa tcctttatcg acaccgcccg gagatatcaa atccccgagc gctatcccct
     3601 ggaactgatc aagggggtgg agatggacgc caccttcaaa ggctataaaa gctatgacga
     3661 tctccacctc ttcgcctggc gggtcgccgg cgtggttggc ctgatgatga cctgcctgct
     3721 cggctaccgg gacgaaagcg cctttcccta cgcggaaaag ctgggcatcg ccatgcagct
     3781 gaccaacatc ctgcgcgata tccaggagga caagcagctg ggccggatct accttccggc
     3841 cgacgagctg caccggttcg gggtggcgcc ggaggatatc ctttcggagc ggatgacccc
     3901 gcagctgcgc gccctgatgg aatttcaggt ccagcgcgcc cacgtctgct atgccgaggc
     3961 cgagcccggc atagcccttc tcgaccgcga atcgcgtttc tccatctaca ccgccagccg
     4021 cctttatcag gagatcctaa aaaagatcga gctggccggt tacaatccct ttgccggacg
     4081 ggccttcgtg cccaccaaac acaaactggc cattctgctg caggagtatg cgcgccggcg
     4141 ctgaacaagg cctccgagga gaggacgccc ccttgataac cgcccgtcac agcaaagcgg
     4201 cgcaagccct ctttcacctc tatatcatgc cggcgatgca gcggcagttt cacagcgtgc
     4261 atctgctcgg cgatgagccc gcctggccgg ctcacacccc ccttatcatc ctgcccaacc
     4321 acagcacctg gtgggatggc tttgtcatct acctgctcaa catccggctc tggcgccggc
     4381 agccctgggt gatgatgctc gaggagcagc tccgcaagcg cccgtttttc cgccgactcg
     4441 gggtcttttc catcgatccc gccacccccg gcggggtgcg ccgctcgcta cgctacaccc
     4501 gcgagctgct cagcggcgag gaagcgggcg ggcggatgct ctgtctcttt ccccagggcg
     4561 agctgctgcc ctgggatcac cggcctctcg gctacaagcc cggattgatc tggctgctgc
     4621 agcgcctcga aagcccggtc tgcctggtgc agctcggcat tcgcggcgag tttctccagc
     4681 agcagcggcc gcagcttttc ctggaatttt cgccgccgct ggagtggtcc ggcggggaga
     4741 ataccctccg ggaatgggag caagcccacg ccgccctgct tgagcgcctc agcggccgca
     4801 tcgccgccgg ggaggagggc cggatcctcc tgcagggccg ccgctctgtg gatgcgcgct
     4861 ggcagtactg gcgaggcctc ctgcgcaggg aggtgctgtg aacggactct tgatcgccct
     4921 ttatgctctt ctgaccatga tctttctggt gagcctcttc aacaccctca ccgcgcccat
     4981 ggtcaggaag ggtccgcagc cgcgccggac gccggtggtt tccctgctcg tccccgcccg
     5041 cgatgaggag cacaccatcg cccgctgcct ggatagcctt tgcgcccagg agtatcccga
     5101 gctcgagatc atcatcctcg acgaccactc tacagaccgc accgccgcca tcgtgggcga
     5161 ccatgccgcc gccgacccac gcctccgcct ggtgcagggc gagcccctgc cggagggatg
     5221 gaccggcaag aactgggcct gccaccagct cagccgggag gcctccggcg agatcctcct
     5281 cttcaccgac gcagataatt ttcacgcccc cttcgccgtc gcccgcactg tgggctggat
     5341 cgaaaaactc gacctcgatc tcttctccgc ctttccgcag cagatcaccc tgagctgggc
     5401 ggaaaagctg gtcgtgccga tcttcgacct cttcgtctac agcctgcttc ccctctggct
     5461 gacctggtac agccgcttcc cctccctctc ggcggccaac ggccagtggc tggccttcac
     5521 ccgccggggc tatgagaaat cgggcggcca cgccgccgtg cgcggccata tcgtcgagga
     5581 taccgccctc agccgccgcg ccaagcagct cggcctcaag actatcactg cctcgggacg
     5641 cggcgccgtc tacggccgca tgtacaccag cccgcgcgag gtctggctcg gcttcgccaa
     5701 gaatgccttc ggactgatga atttccgggc cctgccctat ttcctgttca tgggattcct
     5761 cttttttatc ttcgtcctgc cctatctgct tctgcttgtt ccagcgctgc ggatctgggc
     5821 cctgccggcg gtggggatca atatcctgct gcgtttgatg gtggcgctca agttcggcca
     5881 gcccctcgtt tacagcgtca tcctccatcc gctcagcatc ctcgccacca tcacggtcgg
     5941 gctggcctct ttttactatt tcctgaaggg gaatatcgcc tggaagggaa gagcnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncttgcg ctggtggggt ttcctgaccg
     6121 gggtggtcct ttcagccttt gggttggagt gggccggggt caccaccgga cggattttcg
     6181 gccattatgg ctatggggac aagctgcagc cccagctctt cggggtgccc ctggcgatcg
     6241 gtttcgcctg gctcgctatg gtgctcacct cggcgggggt ggcggactgg ctgccggacg
     6301 cctggcggca cctcttcaga ggtcgacagg ggaatgagtc tgtttctggc gcagacaggc
     6361 cggcttccga ccacggcctg ctgcgcatcc tccttatcgc cctgctcatg ctcctcttcg
     6421 actggatcat ggagccggcg gcggtccggt tgggctactg gagctggcgg gggggcatca
     6481 tccccctgca aaactatctg gcctggtttg ttttcgccct ggtcttcgct gcggcggcct
     6541 ggcggctcgg atttctgcag cggggcgccc ccgccctgag ccggcatgcc tggctcgcgc
     6601 agattctcta cttcctgctg gtccggctgg gatgataatt tggagagtac tatggcaagt
     6661 ttggatggtt ctggcaaatg gcgcggtctg ttcatcgctg cgctgatcta tctggggtgg
     6721 ctcggtcacc tcatcttctg gctgtcagcg gaacttgacc gctccctgct gccgtggata
     6781 gcgccggccc tgctggtgca gacctggctt tacgtcgggc ttttcatcac cgcccacgac
     6841 gccatgcacg gggcggtggt tccgggccgt gagggcctca accgccgggt cggacaggca
     6901 gcagttttca gctacgccct cttttccttt aaaaagctgc tgcagcgcca cggactgcat
     6961 cacagcttcc ccgccagcga gaaagatccc gactttcatg acggcgtgca taccggaccc
     7021 ctgcgctggt acctccattt catgctccac tacctgacca tcccacagct tgtgggcatg
     7081 gcgctgctct tcaatctgct gcagcacggc gcgggggtgc cgctcgccaa cctgctcctc
     7141 ttctgggtcg ccccggccct gctcagcacc tttcagctct tctacttcgg cacctacctg
     7201 ccccaccgcc gtccgcagga gggctatgac aatatgcacc gcgccctctc ctcggattat
     7261 ccggtctggc tctccctgct cacctgcttc catttcggct accaccttga acaccatgtt
     7321 cagccgacgg tgccatggtg gcggctgccg cggattcacc ttcccggcga acgctgaggc
     7381 tcacgccccc ggcctccggt cacagataga tatccacaat caccggatag tggtcggaga
     7441 caaaacggcc gtcccagcgc tccgcgacca ggccgtggtt ggccgcgcgc cggatcccct
     7501 tgacgaagat gaaatcaatc agatagttgg gctggagggc gtcgttaaag ccggtgaagg
     7561 actggctgcc gccgtagtgg ccgttgacgc tgagcatttc gctatccttc atcccctccg
     7621 gctcccgcac cagttcaagg taggcgccct cctggcgggt gctgttgaaa tcgccgctca
     7681 ggatgaccgg gcggccgccg gcaagtgcgg agatcttgcg caggatcagg gcgctgctct
     7741 cctggcgcgc ccgcacgccg atgtggtcaa aatgggtgtt gaaatagaaa aagtctttgc
     7801 cggtggcgcg gtcgcggaac tccgcccagg tggcgatgcg cggcagggcg gcatcccagc
     7861 cgcggctgcc ggcgctgtcc ggagaggggg agagccagaa ggtgccctgg cggatcagcc
     7921 gcagccggtc gcggcgatag aaaatcggca catattcgcc ttcttcgcgg ccgtcatcgc
     7981 ggcccacgcc gatccagtca tattcgggca gcagcccggc cagcaccagc acctgacggt
     8041 tgagcgcctc ctgcaggccg gccacatcga cgcggtgaaa ggaaaaggtg cgcgcgacca
     8101 tctcctggcg cagcggccag gtgttgccgc cgtcatcggg agtgtcgtag cggatgttga
     8161 agctcatgca gcgcagcggc tgatcggggc ctttggcgca ggcgcagatc agacacaggg
     8221 tgatcaggaa catcgcgatc aggccggctt ttttcatcgt tctctcctgt agatcagtta
     8281 tatctccata tcccgtccat tccctcggat gcggctggtt gttcccagtc gatcgcatgc
     8341 ccgaggcctt atttcaacaa cagcgccttg cgcagcacct ggcgatccgg cgatttcaga
     8401 cggatgaaat acaagccgct ggcggccttg tctgcgatcc agcgcacctg atgcaggccg
     8461 gctggcaggg cgcctccggc cagagtggcc acctccgccc ctttcacgtc ataaatcctc
     8521 agggaaatct cctggccaac cggcagttca aagccgatcg tggtcgacag attgaaggga
     8581 ttgggatagt tctgctggag cagaaatcct tgcggcagct cctggtcatc cgcgccgctg
     8641 attcccgcaa gcttgtcgcg cagctgtttt tcgggctctt ccccgccatc ggcgaatttc
     8701 cactccgacc cctgcttgaa aattatggta tttgtattgt cgatgagctg ggcgtcaaag
     8761 gcgatccccc tcgcatagtc aaaggcgtgc atctcgaccg cgatctggtt ctcgccggtg
     8821 cgcaaaaact gcaggccgtt agcggcattg atcaccacca tcttgctcag gttggccgcc
     8881 agggcggtgg cggcgggagt ggcggcggaa atctcctcat ccgccggcag attgatgcgc
     8941 tcgatctcat ggccgttgag gtagaccacg gcgccatcgc ggcccttgac gagcaggccg
     9001 agcccggtca gggcggcggc attggcgacg gtaatacttt ttctgaaata ggccgtctgc
     9061 accgtgccgc agggggtttt aagaccgctg ctggtcccgt agccgacggg tgcagcgccg
     9121 gattgccagc cgccgtcgtc atagcccggg tcctgccagt tgagcaggat tttggtcgta
     9181 tcgatttcga atgcgacagc gaaggaggcg gccatcgcgt ttccccgcag atcggcggcg
     9241 cggaaatagt agagatagtt gcggccgtgc tcgccgctga gggtgaccgc atgtttgcgc
     9301 tggccggtgg ttgtgcatgt ctgctccatc tccccccagc tctcgtcctg gctgctgtag
     9361 cggacggtgg cgtaggtgtc ggtttcgaac tcgagagtga tctcgcggga gtaactggtc
     9421 tgcggtgaat ccggaaatct ggtgaaaacc ggcgggcggg tgtcgtcgta ggggctccag
     9481 gcgtcgaacc cgttgatggc cttgatcagc tcctcggcga tcttgtgatg ggtcgccaca
     9541 ctgggatggc cgtcgtagac ataaccgccc tgataatagg gataaaaggc gtaatagaca
     9601 tcgccattgc cggcggcgtt ctcctcgatg acaatctccg agatcaccag ctgcagccac
     9661 tcgacgtgtg gagcgacggc gaggattttg actccgggat agaggtcgcg cagggtggcg
     9721 ataaatttgt ggtattcgct cttgtacaag gcacggttgc tctcgctgat cgcgccgttc
     9781 cagccgccca tgccggaata atcgttcagg cccagcccaa ttacgaccag atggggagcc
     9841 caggtggaaa agtcccaggg cggggtggcg gtatggatgt gggtttggcc gaagatggcg
     9901 ggaagactgc ctgaacggga gccttgccag tccatgacca tgccgtagcc ggagcggctg
     9961 gtcatcatgt actcggcgcc aaaatggtgc gcagtgatcg ggccaaagcc ctcggaaata
    10021 ttggtgaggg gggcgtcttc cgccggcttg tccggcttgt cgtactcatt gcccgaagcg
    10081 ctggtgtagg aatcgccgac gaattcgatg cggcgctgca gcgcctgttc gggcgggtgc
    10141 aggatcttgc cgtcatcgat gaccaggccg tgaaaagaga atttggccca ggtggtctcg
    10201 ttgcgctttt cgatgcggat ggtgtggagg ccgtcgctca gaccggtcgc gagcatgtag
    10261 gtggtcaagg cggcggcggt ggggtggaaa actgcatggg ggatgccatc gatgataata
    10321 ttgtaatagc agaaattgtc tgtcatcctc accccgatgc tggtcccctc gaaacgggcc
    10381 tggatgcaga ccccgggcca ggagtgggag ggggcgaggg gatcgctgaa atcccagcgg
    10441 cccaggtact ggatgttggg atcgttggcg gg
//
LOCUS       DAOPWU010000097        10459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6414_length_10459_cov_5.060361, whole genome shotgun sequence.
ACCESSION   DAOPWU010000097 DAOPWU010000000
VERSION     DAOPWU010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6414_length_10459_cov_5.060361"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PKM23_06745"
     CDS             complement(<1..497)
                     /locus_tag="PKM23_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91209.1"
                     /translation="MKNRSIRYFLPLMLIAVIFSSVAAQAPLIAERPYEPVVLRGGVL
                     SAHYGYPVNEVHLYAWDGGSGSWRMMPFQIDERIRTIDPNGDGESQRHTYFIADDGLI
                     DDDDELVFMIRDMGEKAPERAWIPNPESKEFNRIELRVYDPDDPALDAYAYLYRSPTI
                     TEAVPA"
     gene            complement(524..1456)
                     /locus_tag="PKM23_06750"
     CDS             complement(524..1456)
                     /locus_tag="PKM23_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNY91210.1"
                     /translation="MTLISLASFLEEAAAQPAAERLRDLGFLEKYLNWTAETTMLFLR
                     SGIRIAIILLITVLLFRAIKALSRRLAGVVERRDLDIEDEKRAETLSLVIRHTLDVLL
                     VAVATITILGELGVQIGPVLAAAGIAGVAVGLGAQSLIKDIINGFFILANDQIRVGDT
                     VQIAGKSGEVEKVSLKLTVLRDVSGNVHFVPNSAIDIVTNMTKDFSRYLLEIGITYSQ
                     DIDRAVEVMKEVDESLRKDPEFDSLIISPLEILGLDRFADSSVIIRARITTLPGKQWK
                     VGREFNRLLKIKFDAEGIEFPFPTCTVYQLKGEN"
     gene            complement(1494..2357)
                     /locus_tag="PKM23_06755"
     CDS             complement(1494..2357)
                     /locus_tag="PKM23_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNY91211.1"
                     /translation="MPLIIEKIYKTKVLPTFTQKRTMRALYKNPFMRRTLNRAAGKKL
                     LRFFGGELHDFCIGGALLAADVEQFLREAKFPYAIGYGLTETSPLIAGTDSRATVLRA
                     TGKPLQGVEMKIDNPHPDTGEGEIVVRGPMVMKGYYRDPERTQAVLSEDGWFRTGDLG
                     VFDKKGYLYIKGRVKNMILGPSGENIYPEEIEEAINEHPDVLESLVYEQGGQICARVY
                     LNYEELDHEFTSLKMSDSEIKAEIGKRLEELRIEVNSRLKSFSRINKIIEQTSPFEKT
                     PTLKIKRFLYA"
     gene            complement(2341..3183)
                     /locus_tag="PKM23_06760"
     CDS             complement(2341..3183)
                     /locus_tag="PKM23_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNY91212.1"
                     /translation="MAHELSSLTLAELLKHSAAAWPQRPALGMVDGMLLTYEALLEKV
                     QLLSAQLQEKGILAGDRVAILSENKPQWGVAYLAITTMGAVVVPILPDFTTGEVQHII
                     RHSGAKAVFVSEKLYSKIEDEFESSLRTIFMIDDFSPVPLQTDKDRLKDFITQGSIGL
                     DRLKEAAMRLAGRGHEVAEEDLASIIYTSGTTGNSKGVMLTHKNIVSDAIATKQIIQL
                     DETDRLVSILPLSHAYECTLGFVLVLYAGASVYYLDKPPTARAAPGPGDDQADDHVLG
                     AAYN"
     gene            complement(3346..4977)
                     /locus_tag="PKM23_06765"
     CDS             complement(3346..4977)
                     /locus_tag="PKM23_06765"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY91213.1"
                     /translation="MKRVITALLLLAAAAQLPAQGKSLQRRYVPIVIKGIMLPLASFD
                     SREWLAFKYQGGRWQAVPFQVDSVQANGGYKHPWPGGRIDENDELVFMPGDLGDRAPA
                     SYWHDIAGERPPQRLEFEFYDQLDPARKGWLYLYRSSQPGPAGYHSHIDAPAGTAADT
                     VVTPSYRLGHNRDGWIDYITLAADPRLDLLDRLKLRLAGNTSAAGGYYALVEDTLNKG
                     ECSPWPGPIRLMRDQRSKLSVPRLLIRDAPIDYRLTYYPFSVTLGVQGEQVNYFIIAL
                     AKAKTLRQSLDLSPAAAGMRFYSEANRGGVAIDGQADGVEANLATQTGMHWAMASGVQ
                     GTFVLVMEMPDIKGGTTRLYYRDSQSGGTEDGTPESGDGRSYGDMGLWAQANSNSDLA
                     MSQITMGFTLYMLPERSRDASFADSLFTWVRQPLGVAIAEQTAPPVGVALAPQQPQQF
                     GISAAWPNPVTAAAGSAQWRITALHPLQAGEVRICNSLGQTVRSWPLAAAGAGDHLLT
                     WDLRDGRGITVAPGIYYLQVRLEGAALTRMVQVLR"
     gene            complement(4977..7112)
                     /locus_tag="PKM23_06770"
     CDS             complement(4977..7112)
                     /locus_tag="PKM23_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91214.1"
                     /translation="MRRFFACLLLLAVPLLAADEGRISGRIFDAVTGQPLPGANIQIA
                     GSVLGATTDNRGHFALLKVPEGRLTLRATYIGYEPALIEIILAAAESREIELRLKPTV
                     IPMHQVVVTGSRQPETLASAAASISVMDRMAMRRRNLLGAAEALQLVSGVTMIGEYIN
                     IRGGSGYNRLGGSRMLVLLDEVPILTSDLGEANWNILPVTEIERMEVLKGAASSLYGS
                     GALSGVVNMITRQPSRELSLSVRQASGLYDRPSVPAWKWTDRTLHFHRTDLSASKSFG
                     PLGLRLALSRHASTGDRENGHFERWYLTGKGVWQLPNNASFSLFSTWSHEERDIFLQW
                     LEQNRALNVPATDRGDSYSLNGYVGYLVYSQVFSPRFSGKARLSFNQQLVGIPFNITS
                     AFTPAIGVSGEGQVSWQPSPAHSLSMGLDYKFDTVESEYYGKRNANGISPYIQEIWKV
                     SNLLHLNAGLRWDTYQLVGDSLEYQVSPKIGFSWQPAYGTILHGSFGRGFRAATVVER
                     FITAGSKDFKAIPNPNLEPERSILFDIGVRQTIDERVYLEATYFFNRYANLIEPTLLA
                     SQLAAQFVNYPVAYIQGVETELRTRFWRDRINLQAAATWMDPRDKQSGGPLLYRPRFI
                     ASLSPFVQFGRFSAGADYRYVGRIEKVAVYPLDERVPARIWDVRLGWRQQGWDFQFIV
                     RNALNYNYTVSERVLGEIRSFMFAISGEI"
     gene            complement(7109..7576)
                     /locus_tag="PKM23_06775"
     CDS             complement(7109..7576)
                     /locus_tag="PKM23_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91215.1"
                     /translation="MVRYIAAAAAAFLLLLPACEIDHGLGTIESRISGRVVFLHPELK
                     PARVDAVRVVAVVNFPPQGLGDLVFTNTSINLSQPAPAYSLPAPLATYALVGAIYREK
                     GREWDYSNILGFYGANPDSNIYSVEEVILSKEHPVAEGIDIYCDWAMVSRVGR"
     gene            complement(7701..8534)
                     /locus_tag="PKM23_06780"
     CDS             complement(7701..8534)
                     /locus_tag="PKM23_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNY91216.1"
                     /translation="MADEQQSLLFEDPETPLHLLHLNRPLVFFDLETTGLEVQLDRIV
                     QFAFIRVDPDRQLTEWKELVNPGIPIPPEASRVHGITDAMVADQPLFSAFAPRISALL
                     HESDLAGFNAARFDVPFLQAEMERAGVPLEGGGRRIIDAQVIFHKREPRDLTAAYRFY
                     CRKELQGAHDALADVRATLEVLEGQLARYSDLPRDVARLGAYCTPADDGRWVTPDRKF
                     YWKNNQAVIGFGKHKGSTLQWLHENYPDYLQWLLTTELAEETRAMLLAAMAGNYPRKE
                     K"
     assembly_gap    8665..8764
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8831..9298
                     /locus_tag="PKM23_06785"
     CDS             8831..9298
                     /locus_tag="PKM23_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91217.1"
                     /translation="MRHCISSLVPALLLLLLAAPSALAAESGGFGVGVILGAPTGLSC
                     KAWIGPHRAIDGAASWTTSGGEHSLYLHSDLLRQNHHALHLDPVWLDWHYGVGIAIRF
                     KEETEMGLRIPVGLDYSLTSIPMELFVEIVPVMLLTPDSSFDIDGGIGLRIFF"
     gene            9320..>10459
                     /locus_tag="PKM23_06790"
     CDS             9320..>10459
                     /locus_tag="PKM23_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HNY91218.1"
                     /translation="MNNVLPLTDPFSGRHIGPHPGERDEMLQTIGVPSLDALIDQTVP
                     ANIRLDKPFDTQQPVSEYQLLQDLARTAAKNQLLRSYIGLGFNDTITPPVIQRNIFEN
                     PGWYTQYTPYQAEISQGRLEALLIFQNMVSDLTGMEIANASLLDEATAAAEAMSMLHR
                     VNSKKSRDPHNNVFLVSDKCLPQTIDVLKTRSAPLGIELRVADLHSAALDERVYGLLI
                     QYPDVDGKAEEWSGFIEKAHTAGAMVAVAADLLALALLTPPGEMGADIVLGSSQRFGV
                     PMGFGGPHAAYFATRNEFMRQMPGRIIGVSIDRHGKRAYRMTLQTREQHIRREKATSN
                     ICTAQALLAIMAGMYGIWHGPEGIKAIAGRIHLLTRALAAALAELG"
BASE COUNT         1933 a         3192 c         3300 g         1934 t
ORIGIN      
        1 gccggcacgg cctccgtaat ggtcggtgag cggtagaggt aggcgtaggc atctaatgcg
       61 ggatcgtccg gatcgtagac gcgcagctcg atgcggttga actctttcga ttcggggttg
      121 gggatccagg cccgctccgg ggctttctct cccatgtcgc ggatcatgaa gaccagttca
      181 tcatcgtcat cgatcaaacc gtcatcggcg atgaagtagg tatggcgctg gctttcgcca
      241 tccccgttgg gatcgatggt gcggatgcgc tcatcgatct ggaagggcat catccgccag
      301 ctgccgctcc cgccgtccca ggcgtagagg tgaacctcgt tgacgggata accgtagtgg
      361 gcactcaaca ccccgccgcg cagcaccacc ggttcatagg ggcgctccgc aatgagcggg
      421 gcctgtgcag ccacggagga aaagatcact gcgatgagca tcaaggggag aaaatatcgg
      481 atagatctat ttttcatggg tacctccaga aaacggggcg agtctaattc tcgcccttca
      541 actgataaac cgtacacgtc gggaagggaa actcgatgcc ctcggcatcg aatttgatct
      601 tgagcaggcg gttgaactcc cgccccacct tccactgctt gcccggcaga gtggtgatgc
      661 gcgcgcggat gatcaccgaa gagtcggcga accggtcgag gccgaggatc tcgagagggg
      721 agatgatcag gctgtcgaat tcgggatcct tgcgcagcga ctcatccacc tccttcatta
      781 cctcgactgc gcgatcgata tcctgggaat aggtgatgcc gatctcaagg agatagcggg
      841 agaaatcctt ggtcatgttg gtgacgatat cgatggcgct gttggggaca aagtggacat
      901 ttcctgagac gtcgcgcagc accgtcagtt tgagactgac cttttccacc tcgccgctct
      961 tgcccgcgat ctgcaccgtg tcgccgacgc ggatctgatc gttggcgagg atgaaaaagc
     1021 cgttgatgat atccttgatc aggctctggg ccccgaggcc gactgcaacc ccggcgatgc
     1081 cggccgcagc cagaaccgga ccgatctgga ccccgagctc gcccagaatt gtgatcgtcg
     1141 ccaccgccac cagcagcaca tccagggtgt ggcggatgac caggctcagg gtctccgccc
     1201 gtttctcatc ctcgatatcc agatcccggc gttccaccac cccggccagc cgccgggaga
     1261 gggccttgat cgcccggaag agtagaacag tgatcaggag aatgatggcg atacggatgc
     1321 ccgagcgcag gaagagcatg gtcgtctcgg cggtccagtt gagatacttt tcgaggaaac
     1381 cgaggtcgcg caaccgctct gcggccggct gggcggccgc ctcctccaga aatgatgcta
     1441 acgagatgag tgtcatggca tactcgtatt aagtccgtca atcgcccttt tccctaggca
     1501 taaaggaaac gcttgatctt gagcgtgggg gtcttttcaa agggcgaggt ctgttcgatg
     1561 atcttgttga tgcgcgaaaa gctcttcagg cggctgttga cctcgatgcg gagctcctcc
     1621 agccgcttgc cgatctccgc cttgatctcc gaatcactca tcttgagcga ggtgaactcg
     1681 tggtccagtt cctcgtaatt gaggtagacg cgcgcgcaga tctggccacc ctgttcgtag
     1741 accagggact cgagcacatc ggggtgctcg ttgatcgcct cctcgatctc ctcgggatag
     1801 atgttctcgc cgctgggccc gaggatcatg ttcttcaccc tgcccttgat atagagatag
     1861 ccctttttat caaagacgcc gagatccccg gtgcggaacc agccatcctc ggagaggacg
     1921 gcctgggtgc gctccggatc gcggtaataa cccttcatta ccatggggcc gcgcacgacg
     1981 atctcgccct cgccggtgtc cgggtgggga ttgtcgatct tcatctccac accctgcagg
     2041 ggcttgccgg tggcgcgcag gaccgtggcg cgcgaatccg tgccggcgat gaggggcgag
     2101 gtctcggtga ggccatagcc gatggcgtag ggaaatttgg cctcgcgcag gaactgctcg
     2161 acatccgccg ccagcagggc tccgccgatg cagaaatcgt gcagctcccc gccgaagaaa
     2221 cgcagcagct tcttgcccgc cgcccggttg agggtgcggc gcatgaaggg attcttgtag
     2281 agggcgcgca tggtccgctt ctgggtgaag gtgggcagaa ccttggtctt gtagatcttt
     2341 tcaattataa gcggcaccga gcacatgatc gtcggcttga tcatctccag ggccgggagc
     2401 agcgcgcgcg gtgggcggct tgtccagata atagaccgag gctccggcgt agagtaccag
     2461 gacgaagccc agggtgcatt cataggcgtg cgagaggggc aggatcgaga ccaggcggtc
     2521 ggtctcatcc agctggatga tctgtttggt ggcgatggcg tcgctgacga tgttcttgtg
     2581 ggtgagcatc acccctttgg agttgccagt ggtgccggag gtatagatga tcgaggcgag
     2641 atcctcctcg gcgacctcgt gaccgcgccc ggccagacgc atggcggcct ccttcaggcg
     2701 gtccaggccg atgctcccct gagtgatgaa atccttgagc cgatccttgt cggtctgcag
     2761 cggcacggga gaaaaatcat cgatcatgaa gatggtgcgc agggaggact cgaattcgtc
     2821 ctcgatcttg ctgtaaagct tttccgagac aaagaccgcc ttggcaccgg aatggcggat
     2881 gatgtgctgc acctcgccgg tggtgaaatc ggggaggatg ggcacgacca ccgcgcccat
     2941 ggtggtgatg gcgagataag ccacgcccca ctgcggcttg ttttcgctga ggatggccac
     3001 gcggtcgccc gcaaggatgc ccttctcctg cagctgggcc gagaggagct gcaccttttc
     3061 cagcagggcc tcgtaggtga ggagcatgcc gtccaccatg cccagagccg gccgctgagg
     3121 ccaggctgcg gccgagtgct tgagaagttc agccaatgtc agcgaagaga gttcatgtgc
     3181 catatgattt tcctgtataa taacggcttc gtttgattca atatagccat ccggcgcgaa
     3241 aaagacaaac gaaatgaatt tgcttcacct ctggcggccg ggcggccctg gcctcgatgc
     3301 accaggcggc cgcggcccgc actgcagtct ttcaagcccg tggattcagc gcagcacctg
     3361 gaccatccgc gtcagtgccg ccccttccag ccgcacctga aggtaataga tccccggggc
     3421 cacggtgatg ccgcgcccgt cgcgcaggtc ccaggtcaga agatgatccc ccgcgccggc
     3481 ggccgccaga ggccagctcc tcaccgtctg gccgaggctg ttacagatgc gcacctcgcc
     3541 cgcctgcagg gggtgcaggg cggtgatccg ccactgcgcc gatccagcgg ccgccgtcac
     3601 cggattgggc caggcggcgc tgatcccgaa ctgctgcggc tgctgtggag cgagggccac
     3661 gccgaccgga ggagcggtct gctcggcgat ggccaccccc aacggctgcc gcacccaggt
     3721 gaacaggctg tcggcgaagg aggcgtcccg gctgcgctcg ggcagcatgt agagggtgaa
     3781 gcccatggtg atctgggaca tcgccaggtc ggaattgctg ttggcctggg cccagagccc
     3841 catgtcgccg taggaacggc cgtccccgct ctccggggtg ccgtcctcag tcccgccgga
     3901 ctggctgtcg cggtagtaga gcctggtcgt gccccccttg atgtcaggca tctccataac
     3961 cagtacaaac gtgccctgca cgcccgaggc catcgcccag tgcatgccgg tctgggtcgc
     4021 gaggttggcc tcgaccccat cggcctggcc atcgatcgcc acaccgccgc gattggcctc
     4081 ggaatagaac ctcatccccg ccgcggcggg ggagagatca agggactggc gcagggtctt
     4141 ggctttggcg agtgcaataa tgaagtaatt cacctgctcc ccttgcaccc cgagggtgac
     4201 cgaaaagggg taataggtga ggcggtaatc gatcggggca tccctgatca gcagcctggg
     4261 gacggagagc ttggagcgct ggtcgcgcat cagccggatc ggccccggcc agggcgagca
     4321 ctcccccttg ttgagggtat cctcgacgag ggcataataa ccaccggcgg cactggtgtt
     4381 gcccgccagg cgcagcttga ggcggtcgag gaggtcgagc cggggatcag ccgccagggt
     4441 gatataatcg atccagccgt cgcggttgtg gccgagccgg tatgaggggg tgacaaccgt
     4501 atcggccgct gtgcccgccg gggcgtcgat gtggctgtga tagcccgccg ggcccggctg
     4561 gctgctcctg tagagataga gccatccctt cctggcggga tcgagctggt cgtagaactc
     4621 gaactcgagg cgctgcggcg gccgctcgcc ggcgatgtcg tgccagtagc tcgccggggc
     4681 ccggtcgccc agatccccgg gcatgaagac cagctcgtcg ttctcgtcga tgcgcccgcc
     4741 cggccaggga tgcttgtagc cgccgttggc ctggacgctg tcgacctgaa agggcaccgc
     4801 ctgccagcgg ccgccctgat atttaaaggc gagccattct ctcgaatcga aactggccag
     4861 cggcagcatg atccccttga tgacgatcgg cacatagcgg cgctgcaggc tcttgccctg
     4921 cgccggcagc tgggccgccg cggcgaggag cagcagggcg gtgataactc ttttcatcat
     4981 atctctccac tgatggcgaa cataaagctg cggatctccc cgagcacgcg ctcggagacg
     5041 gtataattat agttgagggc gttgcgcacg atgaactgga aatcccagcc ctgctggcgc
     5101 cagccgaggc gcacatccca gatcctggcc ggcacgcgct cgtcgagtgg atagaccgcc
     5161 accttttcga tgcggccgac gtagcggtaa tcggctccgg cggaaaagcg gccgaactgc
     5221 acaaagggcg agagagaggc gatgaagcgc ggccggtaga gcagggggcc gcccgactgc
     5281 ttgtcgcgcg ggtccatcca tgtggccgcc gcctggagat tgatacggtc ccgccagaag
     5341 cgggtgcgca gctcggtctc cacgccctgg atgtaggcga ccggatagtt gacgaactgg
     5401 gcggccagct gggaggcgag cagggtcggt tcgatcagat tggcgtagcg gttgaaaaag
     5461 taggtcgcct cgaggtaaac gcgctcgtcg atggtctggc gcaccccgat atcgaagagg
     5521 atggagcgct cgggctcgag gttgggattg gggatggcct tgaaatcctt cgagccggcg
     5581 gtgatgaagc gttcgaccac cgtggcggcg cggaagccgc ggccgaatga gccgtggagg
     5641 atggttccat atgcgggctg ccagctgaag ccgatcttgg gactgacctg gtattcgagg
     5701 gaatcaccca ccagctgata ggtatcccag cgcaggccgg cgttgagatg aagcaggttc
     5761 gagaccttcc agatctcctg gatgtagggc gagatgccgt tggcgttgcg tttgccgtaa
     5821 tactcggact cgacggtgtc gaatttataa tccaaaccca tcgacaggct gtgggccggc
     5881 gagggctgcc agctcacctg cccctcgccg ctgaccccga tcgcgggggt gaaggcgctg
     5941 gtaatattga aggggatgcc caccagctgc tggttgaagg agaggcgggc cttgccagag
     6001 aagcggggcg agaaaacctg gctgtagacc agatagccga cgtagccgtt gaggctgtag
     6061 ctgtcgccgc ggtcggttgc aggcacattg agagcccggt tctgttccag ccactggaga
     6121 aagatgtcgc gctcctcgtg actccaggtc gagaagagcg agaacgaggc gttgttgggc
     6181 agctgccaga cccccttgcc ggtaagatac cagcgctcga aatggccgtt ctcgcggtcg
     6241 ccggtggagg cgtggcggga tagggccagg cgcagcccga gcggcccaaa actcttcgag
     6301 gcgctgaggt cggtgcgatg gaagtgcagg gtcctgtcgg tccatttcca tgccggcacc
     6361 gagggtcggt cgtaaaggcc ggaagcctgg cgcaccgaca gggagagctc gcggctgggc
     6421 tgccgggtga tcatgttgac caccccgctc agggcgcccg aaccgtaaag agatgaggcg
     6481 gctcccttga gcacctccat gcgctcgatt tcggtgaccg gaaggatgtt ccagttggcc
     6541 tcgccgaggt cgctggtgag gatcggcacc tcgtcgagca ggaccagcat gcggctgccg
     6601 ccgaggcggt tgtagcccga gccgccgcgg atgttgatat actcgccgat catggtgacg
     6661 ccggagacca gctgcagggc ctcggcggct ccgaggagat tgcgccgccg catcgccatg
     6721 cggtccatga cgctgatgct ggccgcggcc gaggcaaggg tctcgggctg gcgcgatccg
     6781 gtcaccacca cctggtgcat gggaatgacc gtcggtttga ggcgaagctc gatctccctg
     6841 ctctccgcgg cagccaggat gatctcgatg agagccggct cgtagccgat gtaggtggcg
     6901 cgcagggtga gccttccctc cggcaccttg agcagggcaa aatggccgcg gttatccgtc
     6961 gtcgccccca gcaccgatcc ggcgatctgg atgttggcgc cgggcagggg ctggccggtg
     7021 accgcatcga atattcgccc gctgatgcgc ccctcgtccg cagccagcag cgggacggcg
     7081 aggagcagca gacatgcgaa aaaccttctc atcggccgac ccgggacacc atcgcccagt
     7141 cgcagtagat atcgatgcct tccgccacgg gatgctcttt gctcaggatc acctcttcga
     7201 cggagtagat gttgctgtcg ggattggcgc cgtaaaaacc gagaatattc gaataatccc
     7261 actccctgcc cttttcgcgg tagatggcgc cgaccagtgc gtaggtggcc aggggagcgg
     7321 gcagactgta agcaggcgcc ggctggctga gattgatcga ggtgttggtg aagaccaggt
     7381 cgccgaggcc ctgcggcggg aaattgacca ccgccacgac gcggacggcg tcgacgcgcg
     7441 cgggcttgag ctcgggatgg agaaaaacca cccgccccga aatccgcgac tcgatcgtgc
     7501 cgaggccgtg atcgatctcg caggccggga gcagaagcag gaatgccgct gcggccgctg
     7561 cgatatatct aaccattcag cgctcccata tattactccc ccgttccgcc ttgcaggaca
     7621 gccgggggat ggtgccggct tttactcttc aacgccgctg cactccgata gttcgcggcc
     7681 tggatcacaa ttttgtattt ctacttctcc ttgcgcggat agttgccagc catggccgcc
     7741 agcagcatcg cgcgggtctc ctccgccagt tcggtcgtca gcagccactg cagatagtcc
     7801 ggatagttct cgtggagcca ctgcagggtg ctgcccttgt gcttgccgaa cccgatcacc
     7861 gcctggttgt tcttccagta gaacttgcgg tcgggggtga cccagcggcc gtcatcggcc
     7921 ggcgtgcaat aggctccgag ccgggccaca tccctgggca ggtcggagta gcgggcgagc
     7981 tgaccctcga ggacctcgag ggtggcgcgg acgtcggcga gggcgtcgtg cgccccttgc
     8041 agctccttgc ggcagtaaaa gcggtaggcc gcggtcagat cgcgcggctc gcgtttgtga
     8101 aagatcacct gggcgtcgat aatccgccgc ccgcccccct cgaggggcac gccggcgcgt
     8161 tccatctcgg cctgcagaaa aggtacatca aagcgcgccg cattgaagcc ggcgagatcg
     8221 ctctcgtgca gcagcgcgct gatgcgcgga gcgaaggcgg agaagagggg ttgatccgcc
     8281 accatcgcgt cggtgatgcc atggacccgg ctcgcctcgg gcgggatggg gatgccggga
     8341 ttgaccagtt ccttccactc ggtcagctgc cggtccggat cgacccggat gaaggcgaac
     8401 tggacgatgc gatcgagctg gacctcgagt ccggtggtct cgagatcgaa aaagaccagg
     8461 ggacgattga ggtgcagaag atggagaggg gtctccggat cttcaaagag cagggattgc
     8521 tgttcatcag ccatgcgcag ggctttccta gaataaaacc ggtcctggag agagtcctcc
     8581 gccagggcgc gggcggggct ccatatcgtt taacataggt aaaagataga acatattcaa
     8641 acaattattc atgccgggta ccccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnactgtt ttttttggaa aatttggtta atttaagggt gaattatgca tcaacctatg
     8821 tgataacatc atgagacact gcatctcttc cctcgtcccg gcccttcttc tcctcctcct
     8881 ggccgccccc tccgcccttg cggcggaaag cggcggtttc ggcgtgggcg tgatcctggg
     8941 cgccccgacc ggcctcagct gcaaggcatg gatcggcccc catcgggcca tcgatggagc
     9001 cgcctcctgg accacctcgg gaggtgagca ttcgctctac ctccacagtg acttgctccg
     9061 gcagaaccat catgccctcc atctggatcc ggtctggctc gactggcatt acggcgtcgg
     9121 catcgccatc cggttcaagg aggagaccga aatgggcctg cgcatcccgg tcgggctcga
     9181 ttacagcctc acctccattc ccatggaact cttcgtcgag attgtcccgg tcatgctcct
     9241 gaccccggac agctcttttg atatcgatgg cggaatcggc ttgcgtattt tcttttaaac
     9301 cttctttcga gaggatgtta tgaacaatgt actgcccctg acggacccgt tttcggggcg
     9361 ccatatcggc ccccatcccg gtgaacggga tgaaatgctc caaaccatcg gcgtcccttc
     9421 cctcgacgcc ctcatcgacc agaccgtgcc ggcgaacatc cgcctcgaca agcccttcga
     9481 cacccagcag ccggtgagcg aatatcaact cctccaggat ctggccagaa ctgcagccaa
     9541 aaaccagctc ttgcgctcct acattggcct cggtttcaat gataccatca cccccccggt
     9601 gatccagcgc aacatctttg aaaatccggg ctggtacacc cagtacaccc cctatcaggc
     9661 cgaaatctcg caggggcgtc tggaagccct gctcatcttc cagaacatgg tcagcgacct
     9721 caccggcatg gagatcgcca acgcctccct gctcgacgag gcgaccgccg cagccgaggc
     9781 gatgagcatg ctccatcgcg tcaacagcaa aaagagccgc gacccccata acaacgtctt
     9841 cctggtctcg gacaagtgcc tgccgcagac catcgacgtt ctcaaaaccc gctccgcccc
     9901 cctgggcatc gaactccgcg ttgccgatct ccactccgca gcgctcgacg agcgcgtcta
     9961 cggcctgctc atccagtacc ccgacgtcga cggcaaggcc gaggagtgga gcggctttat
    10021 cgaaaaggcc cacaccgccg gcgccatggt cgccgtggcg gccgacctgc tcgccctcgc
    10081 cctcctcaca cccccggggg agatgggagc cgatatcgtc ctcggctcga gccagcgttt
    10141 tggcgtgccc atgggcttcg gcggccccca cgccgcctat ttcgccaccc gtaacgagtt
    10201 catgcgccag atgccgggcc gcatcatcgg agtctcgatc gaccgccatg gcaaacgcgc
    10261 ctaccgcatg acactgcaga cccgcgagca gcacatccgc cgagaaaagg cgacctccaa
    10321 catctgcacc gcccaggccc tgttggcgat catggctggc atgtacggga tctggcacgg
    10381 ccccgaaggg atcaaggcca tcgccggccg tatccacctc ctcacccgtg ccctcgccgc
    10441 tgccctcgcc gaactgggc
//
LOCUS       DAOPWU010000098        10363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6508_length_10363_cov_4.523865, whole genome shotgun sequence.
ACCESSION   DAOPWU010000098 DAOPWU010000000
VERSION     DAOPWU010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6508_length_10363_cov_4.523865"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKM23_06795"
     CDS             complement(<1..749)
                     /locus_tag="PKM23_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY91219.1"
                     /translation="MNIRESVEIGLEGIRSHKLRSILTALGIIFGVAAVIAMLSIGEG
                     AKQEALEQIQLMGMNNILINDIPIEDEESGAGRSNLSRGLLLEDAAALQTINPLVAMA
                     IPERSVKEQIYFGQESVEATVVGTLPDYERVMAFTPRLGAFFNYQDVTEARRVCVLGA
                     EVRYALFLFQDPVGERIKIGSQWYTVLGVMEEKPSAGAGSGISDNLNNNIYIPITAAV
                     KRFPRSVFASEIDRIIIQVKDSGRIEEAAAIV"
     gene            complement(765..2132)
                     /locus_tag="PKM23_06800"
     CDS             complement(765..2132)
                     /locus_tag="PKM23_06800"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNY91220.1"
                     /translation="MKKYLEEIKKAWRWFDAHLLVRLGKWRIPVLVLATLLLVFVIYK
                     KTLGSVPDVATTAARFGDFVIDINQSGELYAVKSVSVGVPAKVRGSLRVVALAEDGSL
                     VRTGDFLVQFDTAEASQDLEDRRNEYESALADLASLQASNRSTMAQLNTAYETEQYSF
                     EQAKLRYEQMKYEAPSKQREQELSLKKATLSLEQAQRKIESQEIINKADLTKSNLKLR
                     QARLRYEQKAQELNDLTLRAPIDGMVVLQEIWGANGRAKVKVGDSPFRGQTLVEIPDL
                     ATMMVKAKVNEVFISQVHPDQQAVLSVDALPGQQFYGKVLRVATLASRESDSEVKTFD
                     VEILIDGADGRLRPGMTAQCQIITDRIKDKLFVPLESVVHKDDTTIVYIKKGGFKRRE
                     VQIGPKNSDFIVIEKGLKQGDLVALRDPTLPVAAAGEKSTAAIKEKRSERRAPGGRGA
                     FMIMR"
     gene            complement(2181..3974)
                     /locus_tag="PKM23_06805"
     CDS             complement(2181..3974)
                     /locus_tag="PKM23_06805"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNY91221.1"
                     /translation="MQKRWFWPAFAGLLLLIWLVSAQNGDGSRSEPVWSDLRLSDFSI
                     DAVETGEVEAVHSVDLSPPMEWRMDMQIIAMVPEGTVVKEGDFLVQFDTSELEDRLEL
                     AQNTLTSLLAQRDGLRAEQSARISQLKANIAAATYSEEIAVLQRELLKYEAAVKRMDA
                     ELEEKKARISHIEAQTNLESQEVIHASALSTLRVEIEKSRGEVRELEEKIGNLTLRAP
                     IGGLVVYQEIESGGERKKIAVGDKPRPGEPVISIPNLDTMQVKLRVNEMDAARLSIDQ
                     SARISLEAYPGRSFDGQVARIAKLAQKQDFDSEIKDFEVIVRMSQADSVLKPGMTARV
                     QIALGLEQNVLTAPTGAIFERGGERVVFPKKGYPEPLAVKTGSRSDQRIVVVSTELKP
                     GELLSLTAPDSGWHRMGYAAYVDASLRRGQNLSAAFAEMERRGLTYDYDGNRNRRVIA
                     RAPTGAAGDFEGVQGRIPGMPGNGRPMEIDPARMKQLEEAMRGMQPGPGAPTGISPGD
                     TSMVRRMRQGRRPGGQGFPGGFTPGDTSGMRRMRPGRWPGGQGFPAGAAPGDSSRARR
                     RMTPPTGAGNRRTPGGEGFIQGAPDGSLPGR"
     gene            complement(4197..5426)
                     /locus_tag="PKM23_06810"
     CDS             complement(4197..5426)
                     /locus_tag="PKM23_06810"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNY91222.1"
                     /translation="MKELIVCCCTLLLLTAACGAGTPASKPVKLGVDVLVSDPPPLIQ
                     GKRLGLITNATGLTSGHESTIDALRRLPGIQLVALFGPEHGVRGDAEAGAAVEGYVDA
                     RSGIRVFSLYGRNHKPTPEMLEGVDVLLYDIQDIGSRPYTYIYTMAHGMEAAKENGIP
                     FVVLDRPDPLGGMLVEGPVLDPRFRSGIGLYPIPYVYGLTAGELARLFNEEFGIHCDL
                     SVVAMEGWRRGMAFEETGLMWVPTSPHIPQAQSVRHCAAVGSIGELHQVDVGVGMPGP
                     FEYAGAPWITAEELAGELNGRNLPGVFFRPLHYTPFYGTFKGVKLQGVQIHILDPVRF
                     QPVAVQIHILCALQKLYPERNIYDTSRIDMFNKAMGTDRVWKQICAGAGAEEIIASWQ
                     NGLQQFEPLRQKYLLYR"
     gene            complement(5434..7305)
                     /locus_tag="PKM23_06815"
     CDS             complement(5434..7305)
                     /locus_tag="PKM23_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY91223.1"
                     /translation="MKEKSKKEGGAASRSPWFWIPSLYFAEGIPYVVIMTVSVIMFKR
                     LGLSNTDIALYTSWLYLPWVIKPLWSPVVDILKTKRFWIIAMQLLIGIGFLAIAMAIP
                     LPNYLFYTLAVLWVLAFASATHDIAADGFYMLGLSDDQQAWFVGIRSTFYRFAMITGQ
                     GLLIILAGYIEGHSGLSPVTLPVQAGPQTAAVQMFHPDSLAAATPGGGLRMICAPERL
                     IISSELQPKARIDSLFRFTHAWNLEHGFYPAESTALKKEEGGPSWWSRSVARPLEDCL
                     RRHFGEERELAALGEARAGNVGAVYFRLSQPPEPGKEVVVNFSRDAGDKSINLVEGSR
                     LVFTESNWNKPAIALIQLDPKLQSEATGSYVARAGNIPLSWSVTFIVLGILFLIFFFY
                     HGFALPRPASDVGVQSRNFLREFFNTFVSFFRRKHIGAILAFLLLYRFGEAQLVKLIH
                     PFMLDSRELGGLGMTTGEVGLVYGTIGIIALTIGGLLGGFVVPRRGLKFWLFPMLFII
                     HLPDLAFIYLSQAQPDNLWVVSLFVALEQFGYGFGFTAYMLYMIYVAGEGEHKTAHYA
                     ICTGLMALGMMVPGMFSGWLQEIIGYRHFFIWVVLATIPSFIAAYFIPLEQGFGKKK"
     gene            complement(7379..7948)
                     /locus_tag="PKM23_06820"
     CDS             complement(7379..7948)
                     /locus_tag="PKM23_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNY91224.1"
                     /translation="MLVRAKVERVTLDTATSRFVVILKDEKQSRWLPIVVGSTEAQAI
                     ALQLENIAPPRPLTHDLIRNLLEMLTITVEKVVVSDLRDNTYFAIITMTNNGRAKEID
                     ARPSDAIAIALRMNAPIFVVEEVMRKAAIMDGVPPSEDADDEQAPGDRLEQLNIDLIN
                     AVQEERYEEAARIRDEIKRVKSHLREKTT"
     gene            8144..8608
                     /locus_tag="PKM23_06825"
     CDS             8144..8608
                     /locus_tag="PKM23_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91225.1"
                     /translation="MLIFEKAVIKKYWPADDKGADDLIVRQVQMQVEAEIDDSDQIRE
                     LYKNMVRGLVRLSIMDNLTGEEYQIPAVTIRPFTIKQKKTTLGKGDTAETVKQEFVLL
                     TLVCRTKDEDGAAMLAELYRFFNIEVQLNFEEFRYPAIGSGTDEAGDEAIQE"
     gene            8649..9008
                     /locus_tag="PKM23_06830"
     CDS             8649..9008
                     /locus_tag="PKM23_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91226.1"
                     /translation="MPPEVKKVLLDHFQLVALFGTFVTWLAFKGSSSSALRLGRRLSL
                     LAFFIFLYSYFRDYTPRLIAKFDIVEADIWFRRNFAMNKGTDWVEGFLYGASVTLFFG
                     IVLAFSAMELTAKKKKS"
     gene            complement(9013..>10363)
                     /locus_tag="PKM23_06835"
     CDS             complement(9013..>10363)
                     /locus_tag="PKM23_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNY91227.1"
                     /translation="SLRLAAGVIHGRPEPEKIVVLSAVSGVTNHLLRSVGEAMQSERR
                     IGGLLDYLNLLHRELILGTISDSRIQHRVLDEVAYLISRLEKLLYGIAYTGECSRRMR
                     DLIISMGERMAVQLLAGALQQQGLEAQALDADKIDIVASGEFGNGNAELETTARMLPQ
                     HLHDLLRRGGIPVITGFFGRTPGGHTITFGRGGTDYSAAIIAHAMEAEELQIWKDVDG
                     FLTANPEIVPEARPLDYLAYDEAAELSYFGAAILHPRTVEPLSRKGIPAVIRNTYRPD
                     APGTRIGAEKQQHQEIIKSVVTNSDIGMLRLHGASLGQQVGFLKTAVSALSDAEINII
                     SVITAQTAVNLLLGKGDVERSARLVEGLHIAYVDQIEPVTDVALIGVVGEGLDTTRGL
                     AARVFKAVAGAGANVEMIASGASRVTQYFIIKERDRDATVRAIHSEFFGSPAQAVRR"
BASE COUNT         1989 a         3121 c         3220 g         2033 t
ORIGIN      
        1 acgatcgcgg ccgcctcctc gatgcggccg gaatccttga cctggatgat gatgcggtcg
       61 atctcgctgg cgaacacgga gcgggggaag cgcttgaccg cagcggtgat ggggatgtag
      121 atattgttgt tgagattgtc ggaaatcccc gagccggcgc ccgcgctggg cttctcctcc
      181 atcactccga gcacggtata ccactggctg ccgatcttga tgcgctcacc gaccggatcc
      241 tggaagagaa agagggcgta gcggacctcg gcgccgagga cgcaaacccg gcgcgcctcc
      301 gtgacatcct gataattgaa aaaggcgccc aggcgtgggg tgaaggccat cactcgctca
      361 taatcaggca gggtaccgac cacagtcgcc tcgacactct cctggccgaa atagatctgc
      421 tccttgaccg aacgctccgg gatggccatg gccaccaggg gattgatcgt ctgcagggcg
      481 gcggcatcct cgagcaaaag cccgcgcgag agattgctgc ggccggcacc gctctcttca
      541 tcctcaatcg ggatatcgtt gatgaggatg ttgttcatgc ccatgagctg gatctgctcg
      601 agggcctcct gcttggcccc ctccccgatg gagagcatgg cgatgactgc agccacgcca
      661 aagatgatcc cgagggcggt gaggatcgag cgcagtttgt ggctgcgaat gccctccagg
      721 ccgatctcga cactttcacg tatattcatg tggactcttt ctgttcagcg catgatcata
      781 aaggcgccgc ggccgccggg cgcccggcgt tcagaccgct tctccttgat cgcggccgtg
      841 ctcttttccc ccgcggcggc gaccggaagg gtgggatcgc gcagagccac cagatcaccc
      901 tgcttgagtc ccttttcgat gacgatgaaa tcgctgttct tgggaccgat ctgcacctcc
      961 cggcgtttaa aaccaccctt cttgatgtaa acgatggtgg tgtcgtcctt gtggaccacg
     1021 ctttcgaggg gcacgaaaag cttgtccttg atgcggtcgg tgatgatctg acactgggcg
     1081 gtcatgccag ggcgcaagcg cccgtcggct ccgtcgataa gaatctcaac atcaaaggtc
     1141 ttgacttccg agtcgctctc acggctggcg agggtggcca cacgcagcac cttgccgtag
     1201 aactgctggc cggggagggc gtcgacggag aggaccgcct gctggtcggg gtgcacctgg
     1261 ctgataaaaa cctcgttgac cttggccttg accatcatgg tcgcgagatc ggggatctca
     1321 accagggttt ggccgcggaa gggactgtcg ccgaccttga ccttggcgcg gccgttggcg
     1381 ccccagatct cctgcagcac caccataccg tcgatggggg cgcgcaaggt gagatcattc
     1441 agctcctggg ctttctgctc ataacggagc cgggcctggc gcagcttgag gttgcttttg
     1501 gtcaggtccg ccttgttgat gatctcctgg gattcgatct tgcgctgggc ctgttccagt
     1561 gagagggtgg cctttttaag gctgagttcc tgctcacgct gtttggaggg ggcctcgtat
     1621 ttcatctgtt cgtagcggag tttggcctgc tcaaaagagt attgttcggt ctcataggcg
     1681 gtattgagct gggccatggt cgacctgttc gaggcctgca gactggccag atcggcaagg
     1741 gccgattcgt attcattgcg acggtcctcg agatcctgac tggcttcggc ggtgtcgaac
     1801 tggacgagga aatcgcccgt ccggaccagg gagccgtcct cggccagggc gacaacgcgg
     1861 aggctgcccc tgactttggc ggggacgccc acggataccg atttgactgc atagagctca
     1921 ccgctctggt tgatgtcgat gacaaagtcg ccgaagcgcg cggcagtggt agccacatcg
     1981 gggaccgagc ccagggtctt tttgtagatc acgaagacaa ggagcagggt cgccagcaca
     2041 agcacgggaa tgcgccattt gcccaggcgc accagcagat gagcgtcgaa ccagcgccag
     2101 gcttttttta tctcttcaag gtatttcttc atctgttcgc cttttgtggt taagccaaca
     2161 acagtagatc agcatttacc tcaacgaccg ggcaggctcc cgtcaggagc gccctggata
     2221 aaaccttcac cccccggggt ccgacggttc ccagcgcctg tagggggcgt cattcttctc
     2281 ctggcgcggc tgctgtcacc aggagcagca ccggccggaa atccctgacc gccgggccat
     2341 cttcccgggc gcatgcggcg catgcccgag gtgtcgcccg gcgtaaagcc gccagggaat
     2401 ccctgaccgc cgggccgtct gccctggcgc atgcggcgca ccatcgaggt gtcgccgggg
     2461 gagatgccgg tgggggcacc cggaccgggc tgcatgccgc gcatcgcctc ctcgagctgc
     2521 ttcatcctgg cgggatcgat ctccatcggc ctgccatttc ccggcatgcc cggaatgcgg
     2581 ccctgcacgc cctcgaaatc ccctgccgca ccggtggggg cccgggcgat gacccggcgg
     2641 ttgcggttgc catcgtaatc ataggtcagc ccccgcctct ccatctcggc aaaggccgcg
     2701 ctcaggttct gcccgcgccg caaggatgcg tctacatagg cggcatagcc catgcggtgc
     2761 cagccgctgt ccggggcggt cagggagagc agctccccgg gctttagttc ggtggaaacc
     2821 accacgatgc gctggtcgct gcgcgacccg gtcttgacag ccaggggctc gggatagccc
     2881 tttttgggaa aaaccactct ctcccccccg cgctcgaaga tggctcctgt aggtgcggtg
     2941 aggacattct gctccagccc cagagcgatc tggaccctgg cggtcatgcc gggtttgagc
     3001 accgaatcgg cctggctcat gcgcacgatg acctcgaaat ccttgatttc ggaatcgaaa
     3061 tcctgctttt gcgccagttt ggcgatccgc gccacctggc catcaaagga tcggccggga
     3121 taggcctcga ggctgatgcg ggcgctctga tcgatgctca ggcgggcggc atccatctcg
     3181 ttgacgcgca gcttgacctg catggtatca aggttgggaa tactgatcac gggctcgccg
     3241 gggcggggct tgtcgccgac agcgatcttt ttacgttcgc cgccggattc gatctcctgg
     3301 taaaccacca gtccaccgat gggagcgcgc agggtcagat tgccgatctt ctcctcgagt
     3361 tcgcgcacct ccccccggct cttctcaatc tccacccgca gggtgctcaa ggccgaggca
     3421 tggatgacct cctgggactc gagattggtc tgggcctcga tgtgggagat ccgggccttt
     3481 ttctcctcga gttctgcatc catccgcttc accgccgcct cgtacttgag cagttcgcgc
     3541 tgcaggacgg cgatttcctc cgaataggtc gcggccgcaa tattggcttt aagctgggaa
     3601 atgcgcgcgg attgctcggc gcgcaggccg tcacgctggg ccagcaggga ggtcagggtg
     3661 ttctgcgcca actcaagacg atcctcgagt tcggaggtgt cgaactggac gaggaaatcg
     3721 ccctccttca ccactgtccc ctcagggacc atggcgatga tctgcatgtc catgcgccat
     3781 tccatgggag gagagagatc gaccgaatgg actgcctcga cctcgccggt ctccacggca
     3841 tcgatactga aatcgctcag gcgcagatcg ctccaaacgg gttcactcct ggacccgtcg
     3901 ccgttctgcg cactcaccag ccagatgagc agaagcaggc cggcgaaggc aggccagaac
     3961 cagcgttttt gcatgatctt tttgagcatg aatatacctg tccaatgaat gacgggcggt
     4021 cccggctgga ccgcaatttt gagggatgtg ttttcgctgc gctgggatcg gtgcgcaggg
     4081 ctgggacggg cgccagggta gggccgcctg caatagccgg ggaacggtta atagacgcat
     4141 cagggaagga aatgttcccg cccagccatt tctcccccct tgggtccgcc ccgggctcag
     4201 cggtagagca ggtatttctg ccgcaggggt tcgaactgct gcaggccgtt ctgccaggag
     4261 gcgatgatct cctccgcgcc ggcgccggcg cagatctgtt tccagacccg atcggtcccc
     4321 atcgccttgt tgaacatgtc gattcgggag gtgtcgtaga tgttccgctc cgggtagagc
     4381 ttttgcaggg cgcaaaggat gtgaatctgt accgccaccg gctggaagcg aacgggatcg
     4441 agaatatgga tctgcacccc ctgcagcttg acccccttga aagtaccata aaagggggtg
     4501 taatgaagcg gacgaaaaaa gacgccgggg aggttccggc cattaagctc ccccgccagt
     4561 tcctcggcgg tgatccaggg ggcgccggca tactcaaagg gccccggcat gccgaccccg
     4621 acatcgacct ggtgcagctc gccgatcgag cccaccgccg cgcagtggcg gactgactgg
     4681 gcctgcggga tgtggggcga agtcggtacc cacatcagcc cggtctcctc gaaagccatc
     4741 ccccgccgcc agccctccat ggccaccaca ctcagatcgc aatggatgcc gaattcttca
     4801 ttgaacagcc gcgccagttc gcccgcagtc aggccgtaaa cgtaggggat cggatagagg
     4861 ccgatgccgc tgcggaagcg cggatcgagc accggcccct cgaccagcat acccccgagg
     4921 ggatcggggc ggtcgagcac gacaaagggg atcccgttct ccttggccgc ctccatgccg
     4981 tgtgccatgg tgtaaatata ggtgtaaggg cgtgagccga tgtcctggat atcgtagagg
     5041 agcacatcca caccctccag catctcgggg gtgggcttgt ggttgcgtcc ataaaggctg
     5101 aagaccctta ttccgcttct ggcatcgaca tatccctcaa ccgctgcacc ggcctcggca
     5161 tccccgcgta caccgtgctc ggggccgaag agggcgacga gctggatgcc ggggagccgg
     5221 cgcagggcat cgatggtcga ctcatggccg gaggtcaggc cggtggcgtt ggtgatcagg
     5281 ccgagccgtt tgccctggat gaggggcggc ggatcgctca ccagcacatc cacgcccagc
     5341 ttcaccggct tggaggccgg cgtcccggcc ccacaggccg cggtcagaag cagcaaagtg
     5401 cagcagcaga cgatcagctc tttcattact tcctcacttt tttttgccga atccctgctc
     5461 gagcgggatg aaataggcgg cgatgaagga gggtatggtc gccagcacca cccagatgaa
     5521 gaagtggcga tagccgatga tctcctgcag ccagccgctg aacatgcccg gcaccatcat
     5581 gcccagcgcc atcagtccgg tgcagatcgc gtaatgggcg gttttgtgct cgccctcgcc
     5641 ggcgacgtag atcatgtaga gcatataggc ggtgaaaccg aatccgtaac cgaactgctc
     5701 gagggcgaca aacaagctta ccacccagag gttatccggc tgggcctggg agaggtaaat
     5761 gaaggccaga tcgggcagat ggataatgaa gagcatggga aagagccaga acttgagtcc
     5821 gcgcctcggc acgacaaaac cgcctagcag tccgccaatg gtgagggcga tgatgccgat
     5881 ggtgccgtag accagcccca cctcaccggt ggtcatgccc agtcccccaa gttcacggct
     5941 gtcgagcatg aagggatgga tgagcttgac cagctgcgcc tcgccgaagc ggtagaggag
     6001 caggaaggcg aggatggcgc cgatgtgctt gcgcctgaaa aaggagacga aggtgttgaa
     6061 gaactccctg agaaagttgc gtgactgcac cccgacgtcg ctggccggcc tgggcagggc
     6121 gaagccgtga tagaagaaaa agatgagaaa gaggatgccg aggacgataa aggtgaccga
     6181 ccaggagagg gggatgttgc cggcgcgggc cacatagctg cctgtggcct cactttgcag
     6241 cttgggatcg agttggatga gggcgatggc cggcttgttc cagttggatt ccgtgaagac
     6301 caggcggctc ccctcgacga gattgatcga tttgtcgccc gcatcgcgac tgaaattgac
     6361 caccacctcc ttgccgggtt cgggcggctg tgagaggcgg aaatagacgg cgccgacatt
     6421 gcccgcgcgg gcctcgccca gggcggccaa ctcgcgctct tcgccgaaat ggcggcggag
     6481 acaatcctcc agcggccggg caacgctgcg ggaccaccag gagggtccac cctcctcctt
     6541 cttgagcgct gtgctctcgg caggataaaa gccgtgctca aggttccagg catgggtgaa
     6601 acggaagagg gaatcgatcc tggccttggg ctgcaattcg ctgctgatga tcagccgttc
     6661 cggcgcgcag atcatccgca gaccaccgcc cggcgtggcg gccgccagag agtcgggatg
     6721 aaacatctgc acggccgccg tttgcggtcc ggcctgcacc ggcaaagtca ccggcgagag
     6781 cccggaatgg ccctcgatgt agccggcgag gatgatgagc agtccctgcc cggtgatcat
     6841 cgcaaaacgg taaaaggtgc tgcggatgcc gacaaaccag gcctgctggt catccgaaag
     6901 gccgagcata tagaagccgt cggctgcaat gtcatgggtt gcggaggcga aggcgagcac
     6961 ccagagcaca gcgagggtat agaagagata attgggcagg gggatggcca tggcgatcgc
     7021 cagaaaaccg atgccgatga gcagctgcat ggcgatgatc cagaaacgct tggtcttgag
     7081 gatatcgacc accgggctcc agaggggctt gatcacccag gggaggtaaa gccagctggt
     7141 gtagagggcg atgtcggtat tggagaggcc caaccgcttg aacatgatca ccgataccgt
     7201 catgatgacc acataaggga tgccttcggc gaaatagagg gaaggaatcc agaaccaggg
     7261 gctgcgggag gcggcgcctc cctccttctt gcttttttcc ttcatgcgtg cactcacttt
     7321 cgtggatatg aaaaagcctc attattcaat gaggctcttg ggaaaagagg gaatcgtctc
     7381 aggtggtctt ttcacgcagg tgtgacttga ctctcttgat ctcatcgcgg atgcgagccg
     7441 cctcttcgta gcgctcttcc tggacggcgt tgatgagatc gatattaagc tgctcgaggc
     7501 ggtcgccggg agcctgctca tcgtccgcat cctcggaggg gggcacgcca tccatgatcg
     7561 ccgccttgcg catcacctct tcgacgacga aaatcggagc gttcatgcgc agtgcgatgg
     7621 cgatggcgtc gctcgggcgc gcgtcgatct ccttggccct gccgttgttg gtcatggtga
     7681 tgatcgcgaa ataggtgttg tcgcgcagat cactgaccac caccttctcc accgtaatgg
     7741 tgagcatctc cagcaggttg cggatgagat cgtgggtcag gggacggggt ggggcgatat
     7801 tctcgagctg aagcgcaatg gcttgcgcct cagttgaacc gaccacaatg ggcagccacc
     7861 ggctctgctt ttcatccttg aggatgacca cgaatcggct ggtcgccgtg tcgagcgtca
     7921 cacgttctac cttggctcgg accagcattg cctccacctc ctttgaatcg taaagccagg
     7981 gccaaatacg gttaatgtta actattttta ttctctatgt caagatttat tttggcccgg
     8041 ccgggcgctg ttgccgcccc attccacggc aaataagcgc ttgcattgca gccgattttt
     8101 cattactttt ttatcgtgga accaacaagc caagggagtg gaaatgctca tctttgaaaa
     8161 agcagtgatc aagaagtact ggccggcgga cgacaagggc gctgacgatc tcatcgtacg
     8221 ccaggtccag atgcaggtcg aggcggaaat cgatgacagt gatcagattc gcgaactcta
     8281 caagaacatg gtccggggcc tggttcgcct ctccatcatg gacaacctca ctggcgaaga
     8341 gtaccagatc ccggcggtta ccatccgccc cttcacgatc aagcagaaaa agaccaccct
     8401 cggcaagggc gacaccgcgg agaccgtcaa gcaagagttc gtcctgctca ccctggtctg
     8461 ccgcaccaaa gatgaggacg gcgcagccat gctcgctgaa ctctaccgct ttttcaacat
     8521 tgaagtgcag ctcaatttcg aggagttccg ctatcccgcc attggctcgg gaacggatga
     8581 ggccggggat gaggcaatcc aggagtgagc aagcgcggga ctgcccgccc cggtgaagga
     8641 gcccgaatat gccacctgaa gtaaaaaaag tactgctgga ccattttcag ctcgtggccc
     8701 tcttcggcac ttttgtgacc tggctcgcct tcaagggctc atcctcttcg gccctacggc
     8761 tggggcgacg gctttccctc ctcgcctttt ttatctttct ctacagctac tttcgcgact
     8821 ataccccccg cctcatcgcc aaattcgaca tcgtcgaagc cgatatctgg ttccggcgca
     8881 acttcgccat gaacaaggga accgactggg tggagggctt tctctacggg gcatcagtga
     8941 ccctcttttt cggcattgtt ctggcctttt ccgccatgga gctgaccgcc aaaaagaaaa
     9001 agagctgagc ctttaccgcc gcaccgcctg ggcgggcgag ccaaagaatt cactgtggat
     9061 cgcccgcacc gtcgcgtccc tgtccctctc cttgatgatg aaatactgcg tcacccgcga
     9121 cgcccccgag gcgatcatct ccacattggc gcccgcgcct gccaccgcct tgaagacccg
     9181 cgccgccagc ccccgcgtcg tgtcaagtcc ctcccccacc acaccgatca aggccacatc
     9241 cgtcaccggt tcgatctggt cgacataggc gatatgcaac ccctcgacca ggcgggcact
     9301 gcgctccaca tcacccttcc cgagcagaag atttaccgct gtctgggcgg tgatgaccga
     9361 gatgatgttg atctcggcat cgctcagggc cgagaccgcg gtctttaaaa agccgacctg
     9421 ctggccgagg ctggccccgt gcagccgcag catgccgatg tcggagttgg tgacaacgct
     9481 cttgatgatc tcctggtgct gctgtttttc ggcgccgatg cgcgtgcccg gggcatcggg
     9541 ccggtaggtg ttgcggatca ccgccggtat acccttgcgc gagaggggtt cgacggtgcg
     9601 cggatggagg atagccgcgc cgaaatagct cagctccgcc gcctcgtcgt aggcgaggta
     9661 gtcgagcggt cgtgcctcgg ggacgatttc gggattggcg gtgaggaagc cgtcgacgtc
     9721 cttccagatc tgcagctcct cggcctccat ggcatgggcg atgatggcgg cgctgtaatc
     9781 ggtgcccccg cgcccgaagg tgatggtatg gcccccgggt gtgcggccga aaaagccggt
     9841 aatgaccgga atgccgccgc gccggagcag gtcgtgcaga tgttggggca gcatccgggc
     9901 ggtggtttcg agttcggcgt tgccgttgcc gaattcgcca ctggcgacga tatcgatctt
     9961 gtccgcatcc agcgcctggg cctcgaggcc ttgctgctgc agggccccgg ccaggagctg
    10021 caccgccatc cgctcaccca tcgagataat gagatcgcgc atgcgccggc tgcactcccc
    10081 ggtgtaggcg atgccgtaga gaagcttttc aagacgggag atgagatagg ccacctcatc
    10141 gagtacccgg tgctgaatcc tgctgtcgga gatggtcccg aggatgagct cgcgatggag
    10201 caggttgaga taatcgagca gccccccgat cctgcgctca ctctgcatcg cctcgccgac
    10261 gctgcgcagc agatggttgg tgacgccgct gacagcggac agaacgacta tcttttccgg
    10321 ttcgggccgg ccgtgaatca cccctgccgc caggcggagc gac
//
LOCUS       DAOPWU010000099        10109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6707_length_10109_cov_4.536304, whole genome shotgun sequence.
ACCESSION   DAOPWU010000099 DAOPWU010000000
VERSION     DAOPWU010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6707_length_10109_cov_4.536304"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1831)
                     /locus_tag="PKM23_06840"
     CDS             complement(<1..1831)
                     /locus_tag="PKM23_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNY91228.1"
                     /translation="MMRRCALVLAIILLVSPLVLHSQIPGYGTEIDRRVDELISRMTL
                     EEKIGQLQQYTYGVSPELQQMARTGAIGSFLNVRGAAQTNALQKLAVENSRLGIPILF
                     GLDVIHGYKTIFPIPLAEAASWDPDLAEQAAAVAAREARAAGIHWTFAPMVDIARDAR
                     WGRIAEGAGEDPFLGSRMAVARVRGFQGGNLAAPDRVAACLKHYVGYGAAEGGRDYNT
                     TEISEQTLRNIYLPPFRAGVKGGAATLMSAFNDLNGVPASANPFTLDHILRGEWGFDG
                     FVVSDWNSIGELIPHGVAADCAEAALKSLTAGVEMDMESRCFNQSLSRLIEEGILPLG
                     TLDEAVRRVLRIKYLLGLFEKPYTDPKLEKEVMLARPHLELARLMARESMVLLKNDGN
                     LLPLARKGITVAVIGPLIDSRADLLGTWRCEGDPAQVVSILQGIRTALGDGAAILTAH
                     GCDVTGTKGESIPSAVAAAQKADVVIAVVGEDASMSGEAASRARFGLPGMQEELLRAL
                     KGTNKPIVAVLLAGRPLEIPWAADNIPAILMAWHGGTQAGAAIADVLFGDYSPSGRLP
                     VSWPRSAGQLPLYYNFKNTGRPTTDQKFTSRYLDIASTPQFPFG"
     gene            complement(1843..4066)
                     /gene="glgB"
                     /locus_tag="PKM23_06845"
                     /pseudo
     CDS             complement(1843..4066)
                     /gene="glgB"
                     /locus_tag="PKM23_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
     gene            complement(4148..4777)
                     /locus_tag="PKM23_06850"
     CDS             complement(4148..4777)
                     /locus_tag="PKM23_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNY91229.1"
                     /translation="MRLKVILIGIILVSATFPLHAQEENWGLGVHLVVSLPQQGFDNL
                     SRDGEGIGTKVFYRLNPWFSLRGDLAYLSYGERRNSEMSSYGYWLVTRRNESFQLALG
                     PQLSHQIGRITPYAAAMGGFYYYHTVTTYEDYYSYYSYPYTENSNGQSKLGWSLSSGL
                     LIDLGIGAHLDFGFKLQNIRKVETTIKEVTTRQTGVDYVITLGAVFFRN"
     gene            5033..6214
                     /locus_tag="PKM23_06855"
     CDS             5033..6214
                     /locus_tag="PKM23_06855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNY91230.1"
                     /translation="MTAARVILKPGKEHIIGRGHPWLFSGAIARIEGKPANGGVLPVC
                     RADGRVVAQAAYNHLSDIALRILTLDPGVRIDGAFWRARIRSAAALRTALVPESTTAM
                     RLVNAEGDGLPGLIVDRYGDHLVISISTLLMDRQREELCEILATELVPAGIYERSEGR
                     ARQREGLDTRSGMLWGDPPPARLEVLENNLRFQVDLIEGQKTGFFLDQRDSRELIEKL
                     SRGARVLNCFAYSGGFSLYAARGGARSVTSVELSAHACGLCRANLLLNGYDPALHPVV
                     TADVFDYLPTLEERFDLIILDPPAFAKSQKDVARASRGYKEINLQGIRHLERGGLLAT
                     FSCSNHIDHDLFKKIVLAAAADAGVVVQLLRELEPGVDHPVLLAHEEGRYLKGLLLRV
                     M"
     gene            6268..7533
                     /locus_tag="PKM23_06860"
     CDS             6268..7533
                     /locus_tag="PKM23_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNY91231.1"
                     /translation="MTPLAWSTLVIGLILGFLLSLLIRGFRYRSARELAEEIYRANEV
                     QNAQRFQGMYDQMKSAFGSLSLEALSRSTNEFIKLAQARLSSEREYTSQTLEEKKQLI
                     DHQLERMNGELEQLGELMQSLERDRAEKFGELAQQLQASGAQTAALLQATSTLREALV
                     NSKTRGQWGERMAEDVLRMAGFIENVNYIKQKTVSSGRSRPDFTFFLPRNLQLHMDVK
                     FPLDNYIKYLDAATEIEKERFRIDFLRDVKLRIREITTRDYISSDDNTVDYVLLFIPN
                     EQIYAFVQENDHTLFDDSLRQHVIVCSPMTLFAVLAVVRQSIDNFALERTSLEIMRLL
                     GSFRKQWDEFIKKMESLGKSLSAAQNDYEALVTTRRRMLEMPLQKIELLRGSKETPPA
                     GAIGDAAKNGGVEEDQEGQSGVASTASET"
     gene            complement(7514..8563)
                     /locus_tag="PKM23_06865"
     CDS             complement(7514..8563)
                     /locus_tag="PKM23_06865"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNY91232.1"
                     /translation="MGCSCAPFGRNPSGEEVMLYTLANARGTMVKITPFGGIITSIIV
                     PDREGRMGDVVLGFDALADYTDKVPFFGAIIGRYGNRIAGGRFTLDGKSCTLACNNGP
                     NHLHGGIRGFDKVLWQAENVQADVASLTLTYLSRDMEEGYPGNLSVKVSYTLTDDDRL
                     VIDYSASTDKKSVINLTNHSYFNLAGEGDILDHELFIDADRYTPINEGLIPTGELAPV
                     ENTPFDFRSHQTIGARIGVRDEQLIRAGGYDHNYVLNGKMGELRKAAAAFESRTGRLL
                     EVWTTEPGMQLYTGNFLDGTLTGKEGKVYGYRTGFCLETQHFPDSPNHPDFPTTVINP
                     GEIWTSRTAFRFLTR"
     gene            complement(8627..>10109)
                     /locus_tag="PKM23_06870"
     CDS             complement(8627..>10109)
                     /locus_tag="PKM23_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative collagen-binding domain-containing
                     protein"
                     /protein_id="HNY91233.1"
                     /translation="SWYATFRKGEEAARSYEPESIPKAGYFDAMQGKLVVGPTDKSGR
                     DFRGRGRLEYYSRRYWRFAESGDYFIKIGVSADNLFATADFDGDFTSDDRNDDLVRGW
                     EAHVQDWHPGDPVWQDEKGKGVIGAINYLARQGINGISLLTMNIEGRDQNVFPFTRYN
                     ERLRYDVSRLDQWEIVLEHANRMGVLVQLRTQEGVNQSLLDGGSLDRERQLYYRMLIA
                     RFSHYPALIWDLGESGGGSALSTTSPASPQTPDQRLDMAQYFYEHDPYLHPLLFQNSG
                     EFSELLTTGSKYTAALLRLPVRNTHEHLLHYHGLVEKRGLIWTVTALAPFTRSVSTAG
                     PAAAMEIEELRMNALWGGLMAGGAGVELARITADGRSDLTMRDWRQDEYLWSLGSSAL
                     AFFRRLPVAEMESRDDLVVSRNAWCLCKPGEVYALYLPKGGMVQLDLKEADRLLELQW
                     FDPRTGEYVGESTDIRGGGRVQVGPPPGEAGLDWALVLRQPGS"
BASE COUNT         2040 a         2946 c         3115 g         2008 t
ORIGIN      
        1 agccaaaggg aaactggggt gtggaggcga tatcgagata gcggctggta aatttctggt
       61 cggtggtggg gcggccggta ttcttgaaat tgtaatagag cggcagctgt ccggcgctgc
      121 gcggccagct caccggcagg cgtcctgatg ggctgtaatc gccaaaaagg acatccgcga
      181 tcgccgcgcc ggcctgagtg ccgccgtgcc aggccatgag aatggccgga atattgtccg
      241 ctgcccaggg aatctcgaga ggccgcccgg ccagcagaac agcaacaatc ggtttgttcg
      301 tacccttcaa ggccctgagc agctcctcct gcatgccggg cagtccgaat cgggccctgc
      361 tggcagcctc gccgctcatg gaggcatctt caccgacgac agcgatgacc acatcggcct
      421 tttgggcggc agcgacagcc gagggtatac tctccccctt cgtgccggtg acatcgcagc
      481 cgtgggccgt gaggatggcg gcgccgtctc cgagagcagt acggatgccc tgcaggatcg
      541 agaccacttg cgcagggtcc ccttcacagc gccaggtgcc gagcagatcc gccctgctgt
      601 cgataagggg cccgatgacc gccacagtaa tacctttcct ggccaacggc agaaggttgc
      661 catcgttttt aagcagcacc atcgactcac gcgccatcag tcgggcgagt tcgagatgcg
      721 gccgggccag cattacctct ttctcaagtt tgggatcggt atagggcttt tcgaacaggc
      781 cgagcaggta cttgatgcgc aggacgcgcc gcaccgcctc atcgagggtg ccgagcggca
      841 aaatcccctc ctcaatcaac cgcgagaggc tctggttgaa gcagcggctc tccatgtcca
      901 tctcgacacc ggcggtcagg cttttaagag cggcctcggc gcagtcggcg gcgacgccgt
      961 gcggaatgag ttcgccgatt gaattccagt cgctgaccac aaacccgtca aaaccccact
     1021 ccccgcgcag gatatggtcg agagtgaagg gattggccga ggcgggcacg ccgttgaggt
     1081 cgttgaaggc gctcatgaga gtggctgcgc cgccctttac gcccgccctg aaggggggca
     1141 aatagatatt gcgcaaggtt tgttcggaga tttcggtggt gttgtagtcc cgcccgccct
     1201 cggccgctcc atagccgacg taatgcttga gacaggcggc gacgcggtcg ggagcggcca
     1261 gattcccgcc ctggaaaccg cgcacgcgag cgactgccat gcggctgccg aggaagggat
     1321 cttcgcccgc tccctcggcg atacggcccc agcgtgcatc ccgggcgata tcgaccatgg
     1381 gcgcaaaggt ccagtggatg cccgcggccc gcgcctcgcg ggcggcgacg gctgccgcct
     1441 gttcagccag atcggggtcc caactggcgg cttcagcgag cgggatagga aagatggtct
     1501 tgtagccgtg aatgacatcc agtccgaaaa ggatcgggat gccaagccgg gagttctcga
     1561 cggcaagctt ttgcagggca ttagtctgcg cggcgccgcg gacgttcagg aaggaaccga
     1621 tcgcgccagt gcgggccatc tgctggagtt caggactgac accataggtg tactgctgca
     1681 gctggccaat cttctcctcc agagtcatac ggctgatcag ctcatccacc cgcctgtcga
     1741 tctccgtgcc gtagcccggg atctgggaat ggaggacgag gggcgatacc agcaggatga
     1801 tggcaagcac gagtgcacaa cggcgcatca tgggctctcc tctcattgaa tgacaggttt
     1861 gaagatgacc gtcgccagcg gcggcaggac gagattgatc gaacagggac ggccgtggaa
     1921 gggcacctcc tcggcgtgga gtccgccata gttgcccttg ttggttcccc aataatcggc
     1981 cgcatcggag ttgaaaacct cccggtaaaa tccccgccag ggtacgccga tgcggtaggc
     2041 ttcgcggtaa accggcgtga agttgcagac gaagatcagg ctatcctcgg gacggtcggt
     2101 cttgcgcatg aaggtgatga tgctgttgtc cgaatcctga aaatcgatcc attcgaaacc
     2161 ggcgggatcg aaatcgactt catgcagggc cggctcgctc cgatagagac ggttcagatc
     2221 gcgcaccatc gcttgcagct tctgatgcgg ttcgtactgg agcaggtgcc agtcgaggct
     2281 gcggctctcc gaccactcct gccactggcc gaactcggag cccataaaga ggagcttttt
     2341 cccggggtgg ccgtacataa aagagagcag ggtgcgcagg ttggcgaagc gctgccagaa
     2401 atcacccggc atcttgtcga gaagggagcg cttgccatgg accacctcat catggctgag
     2461 cacgaggata aaattttcgt gaaaagcata gagaagggcg aaggtgatca tgttgtggtg
     2521 atacctgcgg tggatgggat ccttggaaaa gtaggtgaga aaatcgttca tccagcccat
     2581 attccacttc atattaaagc ccagaccacc gagataggtg gggcgggaga cccccggcca
     2641 ggcggtggac tcttccgcca ccgagagcac acccggatag taggagaaga tgatctcgtt
     2701 gagctttttg ataaaatcga tggcctcgag gttctcgcgg ccgccgaact ggttggggat
     2761 ccactccccc gccttgcggg agtagtcgag atagagcatc gaggcgaccg catcgacacg
     2821 aatgccgtcg atatggtatt tgtcgaacca gaagagggcg ttggcgatga gaaagttgcg
     2881 cacctcgctg cggccccagt tgaagatcag ggtgccccag tccatgtgtt cacccttgcg
     2941 cgggtcggcg tgttcataga ggcgcgtgcc gtcgaaaaag gccagtccgt gggcgtcggg
     3001 ggaaatgggc cggcacccag tcgacgatga tgccgatccc gtgctggtgg agatagtcga
     3061 ccatgtactg gaaatcagcg ggaggaccaa agcgcgaagt cggggcgtaa tagccgcaga
     3121 cctggtagcc ccacgaggcg tcgaggggat gttcggcgac gggcagcagt tcgatatggg
     3181 tatagcccat ctcggtgcaa tagtcggcca gctgttcggc cagttcgcga taggtgagga
     3241 agcggtactc ttcctcttgt ttgcgacgcc aggagccgag gtggacctca tagatggaga
     3301 ccggcttttt caggatatcc tgctggcggc gctgatcgag ccaatcctgg tcctgccact
     3361 cgaactggtt cagatccgcg accaccgagg ccgtcttggg gcgcagctcg tgctggaatc
     3421 catagggatc ggccttgagg tagatattgc cgttctgatc cttgatttca tatttgtaga
     3481 cctcaccgat gccgatgtcc gggataaaaa gctcccagat gccggagctg ccgaggacgc
     3541 gcatctggtg tttgccgccg tgccactggt tgaaattgcc gacgatgctg acattgcggg
     3601 cgttgggggc ccagacggcg aagtagactc cgtccacgcc gttaatgaca gtgggatgcg
     3661 ctcccatctt ttcataaatc ttgtggtggt cccccatgcc gaagaggtag aggtccatct
     3721 cccccatttg cggcagaaaa aagtagggat cgtgaacata gcgttcatgc ccgttctcgg
     3781 cgatcacgtg cagctgatag aggcgcagag agctccaatc cggtaaaaag acctcgaaaa
     3841 aatggggatt atgaatgttc tgcatgggat attcgatctt ggactgggga tcccgcacag
     3901 atgcggagcg ggcatccgga agccaggccc ggacgaccca gatctgcttg ccctcgcgtt
     3961 cggcctggtg ggggccgagg atggtaaacg gatcatggtg caaattgcgg gcgatgcgtt
     4021 cgacctccgc cgcgcttacg ctgctcaact gctccttatc attcatagag attttagcct
     4081 catggttaaa tgtcgttcgc cgcgatcccg gcgggtgatt gcagaaggcg tcgccggccg
     4141 ccctctatca gttgcgaaaa aagaccgctc ccagggtgat gacataatcg accccggtct
     4201 gccgggtggt cacctccttg atggtggtct cgaccttgcg gatgttctgg agcttgaagc
     4261 cgaaatccag atgagcgccg atgccgagat cgatgagcag gccggaggag aggctccatc
     4321 ccagcttgct ctgaccgttg ctgttctcgg tgtagggata gctgtaataa gagtaataat
     4381 cttcgtaagt ggttacagta tggtaataat aaaagccgcc catggcggcg gcgtagggcg
     4441 tgatcctgcc gatctggtgg gagagctgcg gccccagcgc aagctggaag gattcattgc
     4501 gccgcgtgac cagccaatat ccgtaagagc tcatttcgct gttgcggcgt tcgccatagg
     4561 agaggtaggc cagatcaccg cggagggaga accaggggtt taagcggtaa aagaccttgg
     4621 tgccgatgcc ttcgccgtcg cgggagaggt tatcgaagcc ttgctgggga agcgaaacca
     4681 cgaggtggac gcccagaccc cagttttcct cctgagcatg gaggggaaag gtggcagaga
     4741 ccagaattat tccaatcaga atcactttta aacgcatggt tcgctcctgt ttccggcgcc
     4801 gttttccgcc tcttgtggtg gctctttcac cttcgcacat ttcaggtttc agccaattta
     4861 caaaatcggc tctagttatc caagtaattt tacaggccga cgggccgctt gattttaata
     4921 atcagaatgg ttacatttaa acatgcggtc ggcaccgtgc ccggtcaata tgctgcgacg
     4981 gggtcccttg ctccccaaac caggcacctt caccctcatt tcaggatgaa aaatgacagc
     5041 tgcacgcgtg attctcaagc ccggcaagga gcacatcatc ggacgcggcc atccctggct
     5101 gttttcgggc gccatcgccc gcatcgaggg caagccggcg aacgggggcg tcctgccggt
     5161 ctgccgggcg gacggccggg tggtggcgca ggccgcctac aatcatctca gcgatatcgc
     5221 cctgcgcatc ctcacactcg atcccggcgt gcgaatcgat ggcgctttct ggcgtgcgcg
     5281 catccgcagc gcagccgccc tccgcaccgc gctggtgccg gaatcgacca ctgccatgcg
     5341 gctggttaat gccgaggggg atggactgcc cggcctgatc gtcgaccgct atggagatca
     5401 tctggtcatc tccatctcca ccctgcttat ggaccggcaa agggaggagt tgtgcgagat
     5461 cctggcaacg gagctggtgc cggcgggcat ctacgagcgc agtgagggcc gggcccgcca
     5521 gcgcgaaggt cttgacaccc gctcgggcat gctctggggc gatcccccgc ccgctcggct
     5581 ggaggtcctg gagaacaatc tgcgctttca ggtcgatctg atcgaagggc aaaagacggg
     5641 cttttttctc gaccagcgcg acagccgtga gctgattgag aagctgagcc ggggtgcgcg
     5701 ggtgctcaac tgcttcgcct acagcggggg attttccctg tacgccgccc gcgggggagc
     5761 ccggtcggtg acttcggtgg aactctcggc gcatgcctgc gggctctgcc gcgccaatct
     5821 gctcctcaac ggatacgatc ccgccctcca tccggtcgtc accgccgacg ttttcgatta
     5881 cctgcccacc ctggaggagc gtttcgacct gatcatcctc gatccccccg cctttgccaa
     5941 atcgcaaaag gatgtggccc gcgccagccg aggctacaaa gagatcaacc tgcagggaat
     6001 ccgccatctg gagcgtggcg ggctgctggc gaccttttcc tgctccaatc acatcgatca
     6061 tgatttgttc aaaaagatcg tcctcgccgc ggcagctgat gcgggcgtcg tcgttcagct
     6121 cctgcgcgag ctggaaccgg gcgtggacca tccggtactc ctcgctcatg aggagggacg
     6181 ctacctcaag ggtctgctgc tccgtgtcat gtaggcccgg cgcctgcctc ccttttcttc
     6241 cggactcatc accgcaaagg ataatagatg actccccttg cctggtccac cctggtgatc
     6301 ggcctgatcc tgggatttct gctctcgctg ctcatcaggg gctttcgcta ccggagcgcc
     6361 cgggagctgg cggaagagat ctatcgcgcc aatgaagttc agaacgccca gcgcttccag
     6421 gggatgtacg atcagatgaa gagcgctttc gggagcctct ccctggaggc cctgtcacgc
     6481 tcgaccaacg agttcatcaa gctcgcccag gcgcggcttt ccagcgagcg ggaatatacc
     6541 agccagaccc tggaggagaa gaagcagctc attgatcatc agctcgagcg catgaacggg
     6601 gagcttgaac aactcggaga gctcatgcaa agcctcgagc gcgaccgggc ggaaaaattc
     6661 ggcgagctgg cccagcagct ccaggcctcg ggggcccaga ctgcggccct gctccaggcc
     6721 acctccaccc tgcgcgaagc cctggtcaac agcaagacgc gcggccagtg gggcgagcgc
     6781 atggctgagg atgtgctgcg catggccggc tttatcgaaa acgtcaacta catcaagcaa
     6841 aaaaccgtca gctccggacg aagccgcccc gattttacct ttttcctccc tcgcaatctc
     6901 cagctgcaca tggatgtcaa attccccctc gataattata tcaaatatct cgatgccgcc
     6961 accgagattg aaaaggaacg cttcaggatc gactttctca gggatgtcaa gctgcgcatc
     7021 cgggagatca ccactcgcga ttatatcagc agcgacgaca atacggtcga ttatgttctg
     7081 ttgtttatcc ccaatgagca gatttacgcc tttgtgcagg agaacgacca caccctcttc
     7141 gatgacagcc tccgccagca cgtcatcgtc tgctcgccga tgaccctctt cgccgtgctg
     7201 gccgttgtgc gccagtccat cgataacttt gccctcgaac gcacctcact ggagatcatg
     7261 cgcttgctcg gctctttccg caagcagtgg gatgaattca tcaaaaagat ggaaagtctg
     7321 ggcaagagcc tctccgccgc ccagaacgac tatgaagccc tggtgacgac gcggcggcgc
     7381 atgctcgaaa tgccgctgca gaaaatcgaa ctcctgcgcg ggagtaagga aacgcccccg
     7441 gccggcgcga ttggagatgc ggcgaaaaac gggggcgtag aagaggatca ggaggggcaa
     7501 tcaggcgtgg catctaccgc gtcagaaacc tgaaggcagt ccgggatgtc cagatttcac
     7561 ccgggttgat cacggtcgtc ggaaaatcgg gatggttggg tgaatcggga aagtgctggg
     7621 tttcaagaca aaagccggtg cggtaaccgt acaccttgcc ctccttgccg gtcagggtgc
     7681 cgtcgagaaa attacctgta tacagctgca tgccgggttc ggtggtccag acctcgagaa
     7741 gcctgccggt gcgggattca aaggccgcag ccgccttcct cagctcaccc atctttccat
     7801 tgagcacata gttatggtca tatccgccgg cgcggatgag ctgttcatcc cgcacgccga
     7861 tacgtgcgcc gatggtttga tgagaacgga aatcgaaggg ggtattttcg accggggcca
     7921 gttcgccggt cgggatcagc ccctcattga tcggggtgta gcggtcggca tcgatgaaga
     7981 gctcgtgatc gaggatatcc ccctcccctg ccagattaaa ataggaatgg ttggtcagat
     8041 tgatgacgct ctttttatcg gtggaagcgc tgtaatcgat caccagcctg tcgtcatcgg
     8101 tgagggtata gctcaccttg acggagagat tgccagggta gccctcctcc atatcccggc
     8161 tcaggtaggt gagagtgagg ctggccacat ctgcttgcac attctcggcc tgccagagca
     8221 ccttgtcaaa tcccctgatg ccgccgtgga gatggttggg tccattattg caggccaggg
     8281 tgcagctttt gccatcgaga gtgaagcgcc cgccggcaat gcggttgcca tatcgtccga
     8341 tgatggcgcc gaaaaagggc accttgtcgg tatagtccgc aagtgcatcg aaaccgagga
     8401 cgacatcgcc catgcggccc tcgcgatcgg gtacgatgat ggaggtgatg attccgccga
     8461 agggggtgat tttaaccatg gtccccctgg cgttggccag ggtgtagagc atcacctcct
     8521 cgccggaggg gttcctgccg aatggcgcac agctgcatcc cattgtttca cctcgcttta
     8581 aagtcaaaaa ccacttctgt cagatagtaa attcccatca gaccgttcaa ctgcccggct
     8641 ggcgcagcac cagcgcccag tccagtccgg cttcgcccgg aggggggccg acctgcactc
     8701 tgccgccccc ccggatgtca gtggattcgc ctacatattc cccggtgcgc gggtcgaacc
     8761 actgcagttc caggagacgg tccgcttcct tcagatcaag ctggaccatg ccacctttcg
     8821 gcagatagag agcgtagacc tcacccggtt tgcagaggca ccaggcattc ctggagacga
     8881 ccagatcatc ccggctctcc atctccgcga cgggcaggcg ccggaaaaag gcgagagcgc
     8941 tgctgcccag gctccagaga tactcgtcct ggcgccaatc gcgcatggtg agatcactgc
     9001 ggccgtcggc tgtgatgcgg gccagttcaa cccccgctcc gccggccata agaccgcccc
     9061 agagggcgtt catgcgcagt tcctcgatct ccatcgcagc ggccgggccg gctgtggaga
     9121 cagagcgggt gaacggcgcc agcgcggtca ctgtccagat caggccgcgt ttttccacca
     9181 ggccatgata atgaagcaaa tgttcatggg tgttccggac gggaagacgc agcagtgccg
     9241 ctgtgtactt ggagccggtt gtcagcagct cgctgaattc gccgctgttc tggaaaagaa
     9301 ggggatggag atagggatcg tgttcgtaaa agtactgggc catatccagc cgctggtccg
     9361 gggtctgcgg actggcaggt gaggtggtag agagggccga gccgccgccg ctctcgccca
     9421 ggtcccagat caatgcagga taatggctga aacgggcgat gagcatgcgg tagtaaagct
     9481 gccgctcccg gtcgaggctg ccgccgtcga ggagggattg attgaccccc tcctgggtgc
     9541 ggagctggac gagcaccccc atacggttgg catgttcgag cacgatttcc cactgatcga
     9601 gccgggagac atcataacgc agcctctcat tgtagcgggt gaaaggaaag acgttctggt
     9661 cccttccttc gatgttcatg gtcagaaggg agatgccgtt gatgccctga cgggcgaggt
     9721 agttgatggc gccgataacc cccttgccct tttcatcctg ccagaccggg tcacccggat
     9781 gccagtcctg gacatgggcc tcccagccgc ggacgaggtc gtcgttgcgg tcgtcgctgg
     9841 tgaaatcacc gtcgaaatcg gcggtggcga acaggttatc cgccgagacg ccgattttga
     9901 tgaaataatc gccgctctcg gcgaagcgcc agtagcggcg gctgtagtac tccagccttc
     9961 cccggccgcg gaaatcgcgg ccgctcttgt cggtggggcc gacgaccagt ttgccctgca
    10021 tggcgtcaaa atatcccgcc ttggggatag actccggctc atagctccgg gcggcctcct
    10081 cgcccttgcg gaaggtggca taccagctc
//
LOCUS       DAOPWU010000100         9967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6821_length_9967_cov_3.962571, whole genome shotgun sequence.
ACCESSION   DAOPWU010000100 DAOPWU010000000
VERSION     DAOPWU010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6821_length_9967_cov_3.962571"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="PKM23_06875"
     CDS             <1..1461
                     /locus_tag="PKM23_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91234.1"
                     /translation="QELILSGRRVRGISGYLHLGLVNVDLVYGELQRAVQTTFTRDDS
                     GRSVPLRYGTFKQSILGVRPSLGDGRVFQFGLTLAKIRDDTSSIKAGYHPRDNLVLGP
                     DVRLALLGGRLVFTGAAALSLLTQDISYGPSTTADIKRIFNSDAELPIDPADLADILI
                     INDSTVPLDPEARSSMAWLLNAQINQWGHAINIGYRSIGDEYLSLANPWLRRDLRGLY
                     IQDRFRLYRNKLFINLGYERYDDNFSRLDGNPAVDMRTINYGLSLFPGAPYPQLSLSM
                     RDQLRDNHLRETSEESIAVSTVLDTLITRDEREKSWFRDLVVQATQDFRLLEAAHQFS
                     AGYISSRNIDEFKEKRAEAFYSPDLSNRVLLVSLTSRYVFPLVTVLSYSSNHSEGASF
                     SAIDFNTWGLTGEYRWLDDRLSATAELRLVGITRQLEATDFETSRNQYRLGVRWNIST
                     GHSVMAEGQLWSYDVPGGDSGDGTDSLFRIRYDRYF"
     gene            1492..3603
                     /locus_tag="PKM23_06880"
     CDS             1492..3603
                     /locus_tag="PKM23_06880"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNY91235.1"
                     /translation="MNRQQARAHWRIVGLVAFLSVFLSFVLSELVMVENLQLKGINTL
                     FNLRGPINPPDTSIVIVALDDQSLASLPAKLPYPRSYYETIVNHLSSAGARLIVFDIE
                     FTEPAVDRPEEDVALAAAVQRSGRVVLAGKMVLETGSRLANNQHILEPVPPLMKSGAA
                     LGLVNVVEDADGFIRNYILYQQASRSTYYTLAVQGARLLAGISSDALPGTGNEPFTIG
                     SQTFPRARTNTFMINFRGPARTFRTYSLVSVLDDSSFTLDEEEDTDIFSQYLEWGTFR
                     DKIVLVGASAEELQDNKLTPFFDWGGVKQKMPGVELHANALSTILRGDFIRSTPEWAA
                     LALALLLTLAAGLQARWLQGLRGLAAVLAVALLFGVAVYLAFSQFRLLIPFATPLIAL
                     ALATFGHVSYQVVVEQREKKLIRQTFQQYVAPSVVDKMLSLGELPSFGGERKELSVLF
                     SDIRNFTRFSEAHEPEVVAARLSEYLSEMVEIIFRQGGTLDKFVGDAIMAIYGAPYYF
                     EDHAERACRTALEMQQRLKELQKGWPDEPFARFNIGVGINTGKMIVGNLGSRQLFDYT
                     VIGDEVNLASRLEGANKEYETGIIISESTCRQISAKALVRELDLVRLVGRSQAIRIYE
                     LRSMDALPQLEQDYLIDLYSEGLAAYRERRWSDALITFRRVLRYFPHDGPSRMYTVRC
                     LNYLERPVPVDWDGVYDMKSK"
     gene            3631..3813
                     /locus_tag="PKM23_06885"
     CDS             3631..3813
                     /locus_tag="PKM23_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91236.1"
                     /translation="MEQEEKLNKPVVGEFKGNPILTLNPDERYPFSFGLSKARLILAH
                     LDDIRAFVEQYSKGKG"
     gene            complement(3885..5189)
                     /gene="ybgF"
                     /locus_tag="PKM23_06890"
     CDS             complement(3885..5189)
                     /gene="ybgF"
                     /locus_tag="PKM23_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNY91237.1"
                     /translation="MKRSIIVVTLAVLLIFTAIAPAQETTTAQPKNSRIGLGFNIGLQ
                     KPYCDVLHTGAGLAGEFMMRYLLSNYFDISLGLGYGTLNDGFSYNTFVTDLIAGDIKA
                     NIHLSKPGNTNPYIFVGAGFTNSSYTRNKWWAIGSPDFEDKRVTLGSFIYGGGVEFML
                     TPQVALNTFMDYRFSTGDALDGAELGKYKDGYLNARAGITYYLSPRAGKAKSDRDDLL
                     ALQQGQLSGAEQSGGTGMDMFEAKLDKMEAGDAELSMEQYVRLKSRVDELNQLISIKE
                     TELDELRATLDFKNQRITDLESTLQSADFAGGSGLSGDFAYNYEEALRAFYARDYRRA
                     ASIFSNLLTQYPSHALASNCQYWIGECMFGLKDYEQASAAFQAVFNFENTTKKDDATL
                     MLGRCFYALNDRSNAREYFQSVIDKYPGSEYIEKARQWLRRL"
     gene            complement(5614..7356)
                     /locus_tag="PKM23_06895"
     CDS             complement(5614..7356)
                     /locus_tag="PKM23_06895"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY91238.1"
                     /translation="MIMRSHIALIILTIWAASASAAQQLQTLLQQEMEAGAISRGEFL
                     AYQLIAAEDPDRLPLRYKTAGRDPLHCSSLITAEVRALLPTLAGEERSLLEQLLARPT
                     SGRLPLSVTSPAGHFRIHYAESGSDSAAADFIAEAGRSFDYVYDLLVHTMGFTPPPSD
                     DGIDGPELDIYVMRFSAYGETRYENPVEGSDGQRYTSYIVIDNRFSGAGYETKGIDAL
                     HVTAAHEFFHMLQGAYRFFPSTRMDSRFLFEASSTWLEDVAYSEVNDYFQYVRSLFAS
                     PNRPFHLFSNITYGLGLYMTMLHKSHGYALIRRMWEELRDQEPLDALDRALRSYGNDL
                     GRSLATFTIWNAFTADYADSLLYYPDSPSFPEITPVEKLTLSGERTILNSCSQLESHY
                     YTLSVAAGGSYTITPVLDPSAQWLYTLVIHEAGYESRHFTIAGETPLTFGPVSMECEL
                     RLAVVNIQWPSAGSSQKKAAYTFRFAPSAHQTEHEDGVVMISPSPFRPEQDERLQIQF
                     YSHEAVSEAALHILSERGEVVYSRIMRSLPRGLNCQFWDGRDSSGRPLPSGIYLCAIS
                     GIGQFKPHKMALVR"
     gene            complement(7358..8686)
                     /gene="der"
                     /locus_tag="PKM23_06900"
     CDS             complement(7358..8686)
                     /gene="der"
                     /locus_tag="PKM23_06900"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNY91239.1"
                     /translation="MKKKPVVALVGRPNVGKSTLFNRIIRKREAIVDDQPGVTRDRKY
                     QPAEWSGFAFDLIDTGGYVAGSRDIFEQAIREQVHYAIEEADVIVFVTDVTTGITAMD
                     EEIARMLQRVQSRVIVAINKVDNEYREADMGEFYRLGLGDPFVISAMSGRSMGDFLDA
                     LTALLPRKEAPAVEEREKEEAVHIAILGRPNVGKSSYVNALLGTDKQIVTPLPGTTRD
                     AVDTRLRYKERQLVLIDTAGLRKRARVKENVEYFSTVRSYHALERCDIAVVLIDASQP
                     IADQDKTILQAAAQAGKGIVLGVNKWDAVEKDNQTAKNFETDIREALRDLAFIPMLFI
                     SALEKQRVFKLLDLALAVYDECQRSVPTPELNRFLQAVVEKNHPPAYGTKWVKLNYIT
                     QAKVNPPLFIIFTNEPRGIKQNYRNFLENQLRAQFGFMGVPIRLAFRLKN"
     gene            complement(8683..>9967)
                     /locus_tag="PKM23_06905"
     CDS             complement(8683..>9967)
                     /locus_tag="PKM23_06905"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HNY91240.1"
                     /translation="MKISVVYPDTPAARLGLQPGDEIIAIDSHEIRDPIDFQFYSAEE
                     SYCLHLRRGGHEWKVNLAPEESGWLGVEFADMEYHSCGNKCVFCFVDQNPPGMRKSLY
                     FKDEDYRLSFLYGNYVTLTNLSRSHLERIVEQRLSPLYVSVHSTEDDLRKRMLGLRRS
                     DRLLEKIDFLARGGIELHAQIVLCPEWNDGEHLEKTLADLAARFPAVQSVAVVPLGLT
                     GHREELTPLRTVTPAEAAGLVVSEKRWEKEFRSRLGIAFVYLADEFYLLAGAPLPSAA
                     RYDGFPQIENGVGMTRSFLDRFARQKYRFPREVKPVRVSIVSGELAAPILTARVLPVL
                     NTISGLQAEVVAVKNRFFGGKVTVSGLITGGDIIGQLRGMERADLIMIPPNCRNGDGL
                     FLDDLDLDGLSRALDAPVLAPQSGFTELFTYLKKL"
BASE COUNT         2048 a         2787 c         3051 g         2081 t
ORIGIN      
        1 caggaactga tcctctcggg gcgcagggtg cgtggaatat ccggctatct ccacctcgga
       61 ctggtcaacg tcgacctcgt ctacggcgag ctgcagcggg ctgtgcagac cactttcacc
      121 agggatgatt cagggcgttc tgtccccctc cgctatggca ccttcaaaca gtccatcctt
      181 ggcgtgcggc ccagcctggg cgacggcaga gtcttccagt tcggccttac gcttgccaaa
      241 atccgcgatg acaccagttc gataaaggcg ggctatcatc ccagggacaa cctggtgctg
      301 ggcccggatg tcagactggc gctgctggga ggaaggctgg tctttaccgg cgcagccgcc
      361 ctaagcctgc ttacgcagga catctcctat gggccttcga ccacagcgga tatcaagcga
      421 atttttaaca gcgatgcgga acttcccata gatccagcgg atctggcgga tatccttatc
      481 atcaatgaca gcaccgtccc cctcgatccc gaggcccgca gttccatggc ctggctcctc
      541 aacgcccaaa tcaaccagtg gggtcatgcc atcaacatcg gctatcgttc gatcggcgac
      601 gagtacctct ccctggccaa cccctggctg cgcagggatc tgcgcggact ctacatccag
      661 gaccgcttcc ggctctaccg caacaaactc tttatcaatc ttggctatga gcgctacgat
      721 gacaacttta gccgtctgga tggcaacccg gcggtggaca tgcgcacgat caattatggc
      781 ctctccctct ttccgggcgc tccctatccc cagctcagcc tgagtatgcg cgaccagctg
      841 cgcgataacc atttgcgtga gaccagcgag gagagcatcg ccgtcagcac ggtgcttgac
      901 accctgatca cccgcgacga gcgcgaaaag agctggttcc gggatctggt cgttcaggcg
      961 acgcaggatt tccgtctgct cgaggcggcc caccagttca gcgccggtta catcagcagc
     1021 cggaacatcg acgaattcaa ggaaaagcgg gctgaagctt tctattcgcc tgatctctcc
     1081 aaccgggtgt tgctggtttc cctgacgagc cggtatgtct ttcctctggt caccgttctc
     1141 agctacagca gcaaccatag tgagggggca agtttctcag ccatagattt taacacctgg
     1201 ggcctgaccg gggagtaccg ctggctggat gaccgtttga gcgccacggc ggagctgcgg
     1261 ctggtcggga tcacccggca gctggaggca acagatttcg agaccagccg caaccagtac
     1321 cgcttgggag tgcgctggaa catcagcacg ggccactcgg tgatggccga ggggcaactt
     1381 tggagttatg acgttccggg aggcgacagc ggggatggga cggacagcct cttcaggata
     1441 cgatatgacc gctactttta aagcttccca tgcagagctg agcaggcggc catgaacagg
     1501 cagcaggcta gagcgcactg gcgcattgtg gggctggtgg catttttatc cgtgttcctc
     1561 tcctttgtcc tgagcgaact ggtcatggtc gaaaacctgc agctcaaggg gatcaacacc
     1621 cttttcaatc tgcgcgggcc gatcaatccg cctgacacct ccattgtcat cgtcgccctc
     1681 gatgaccaga gcctggccag tctccctgcc aaattaccgt atccgcgcag ctattatgag
     1741 accatagtca accatctcag ctctgccggt gccagattga tcgtctttga catcgagttc
     1801 accgagccgg ccgtcgaccg cccggaggag gatgtcgctc tggctgcagc ggtgcagcgc
     1861 agcggccggg tggtgctggc gggcaagatg gtgctcgaga ctggcagccg cctggccaac
     1921 aaccagcaca tcctcgaacc ggtgccgccg ctgatgaaga gcggggccgc cctggggctg
     1981 gtcaatgtcg tggaggatgc cgatggcttc atccgcaact acatcctcta ccagcaggcc
     2041 agccgcagca cctattacac actcgccgtg cagggcgccc gcctgctcgc cggtatcagc
     2101 tccgatgccc tgcccggaac aggaaacgag ccgtttacaa tcggcagcca gaccttcccg
     2161 cgcgcccgca ccaatacctt tatgatcaat ttccgcggtc ctgcccgcac cttccgcacc
     2221 tattccctgg tgagtgtcct ggacgattcc agtttcaccc tggatgagga agaggacacg
     2281 gacatctttt cccagtacct cgaatggggc acctttcggg acaagatcgt cctggtcggg
     2341 gcctcggccg aggagctgca ggacaacaag ctcaccccct tcttcgactg ggggggggtg
     2401 aaacaaaaga tgcccggcgt ggagctgcac gccaatgccc tcagcaccat cctgcggggg
     2461 gatttcatcc gttccacccc ggagtgggcg gccctggccc ttgccctgct gctcactctc
     2521 gcagccggcc tgcaggcgcg ctggctgcag gggctgcgcg gcctggccgc tgtgctggcg
     2581 gtggcgctcc tgttcggtgt cgccgtttat ctcgccttca gccagttcag gctccttata
     2641 ccctttgcca cccccctgat tgccctggct ctggccacct tcggccatgt ctcctatcag
     2701 gttgtggttg agcagcggga gaaaaaactg atcagacaga cctttcaaca atatgtcgcc
     2761 ccttcggttg tagacaagat gctcagtctg ggcgagcttc caagcttcgg cggggaacgc
     2821 aaggagctga gcgtgctctt ttccgatatc cgtaatttca cccgcttcag cgaggcgcac
     2881 gaaccggaag tagtggcggc gcgcctctcg gaatacctca gcgagatggt cgaaatcatc
     2941 ttccgccagg gcggcacgct cgacaagttt gtgggcgatg caatcatggc tatctatgga
     3001 gcgccctatt actttgagga tcacgccgag agggcctgcc gcaccgccct cgagatgcag
     3061 cagcgcctga aggagctgca gaaaggctgg cccgacgagc cctttgctcg cttcaatatc
     3121 ggcgtcggca tcaataccgg caagatgatt gtgggcaatc tgggctcccg gcagctcttc
     3181 gattatacgg tcatcggcga cgaggtcaat ctcgcctccc gtcttgaggg ggccaacaag
     3241 gagtacgaga ccgggatcat catctcggag agcacctgca gacagatcag cgccaaagcc
     3301 ctggtgcggg agctagatct ggtgcgactg gtggggcgtt cgcaggccat ccgcatctac
     3361 gaactgcgca gcatggacgc cttgccgcag ctcgaacagg attatctgat cgatctttac
     3421 agtgagggac tggctgccta tcgggagcgg cgctggagcg acgccctgat caccttccgc
     3481 cgggtgctgc gctattttcc gcatgacggc ccctcacgga tgtacacagt gcgctgcctc
     3541 aattatctgg aaaggccggt cccggtcgac tgggacggcg tttatgacat gaaaagtaaa
     3601 tagctgatta ttaaagaaga ggagtggaac gtggagcaag aagaaaaact taacaagccg
     3661 gtggtggggg aattcaaggg aaatccgatc ctcaccctca atccggatga acgctatccc
     3721 ttttcgttcg gactttcgaa ggcgagattg attctcgcac acctcgatga cattcgcgcg
     3781 tttgtcgaac aatacagcaa gggcaagggc tgagattgga ttgacgggat ccggaaggag
     3841 tacaaaaaaa gggctgcagt tcactgcagc ccttttttaa tggcctagag ccgtctgagc
     3901 cactggcggg ccttttcgat atactcgctg ccgggatact tgtcgatgac cgactggaag
     3961 tactcccggg cgttgctgcg gtcgttgagg gcataaaagc agcgcccaag catcagggtg
     4021 gcgtcatcct ttttggtggt gttttcgaaa ttgaaaacgg cctggaatgc ggcggaggcc
     4081 tgttcgtaat ccttgaggcc gaacatgcat tcgccaatcc agtactggca gttgctcgcc
     4141 agagcgtgcg aggggtattg cgtgagcaag ttgctgaaga tggaggcggc gcggcggtaa
     4201 tcacgagcat aaaaagcgcg caaggcctct tcatagttat aggcgaaatc accgctcagg
     4261 ccgctgccgc ctgcgaaatc agcactctgc agggtggatt cgaggtcggt gatgcgctgg
     4321 ttcttaaagt ccagggtcgc gcgcagttca tccagttcgg tctccttgat cgagatcagc
     4381 tggttgagct catccactct ggatttgagg cggacgtact gttccatgct cagctcggcg
     4441 tcgccggctt ccatcttgtc cagtttggcc tcgaacatgt ccatgccggt gcctccggac
     4501 tgttcggcgc cggagagctg gccctgctgg agggcgagaa gatcatcacg atccgatttt
     4561 gccttgccgg cgcgcgggct cagatagtag gtgatgccgg cgcgggcgtt gagatagcca
     4621 tccttgtatt tgcccagttc ggctccatcg agggcatcgc cggtgctgaa gcggtaatcc
     4681 ataaaggtat tcagggccac ctgcggggtg agcatgaatt ccacgccgcc gccgtatatg
     4741 aaagagccga gggtgacgcg tttgtcttca aagtcggggg agccgattgc ccaccacttg
     4801 ttgcgggtgt aggaggaatt ggtaaagccg gcgccaacga agatataggg gttggtattg
     4861 cccggcttgc tgaggtggat gttggccttg atatcgcctg cgatgaggtc ggtcacaaag
     4921 gtattgtagc tgaagccgtc attgagggtg ccgtaaccga gtccgaggga gatgtcaaaa
     4981 tagttgctga ggagatagcg catcataaac tcgccggcaa ggccggcgcc cgtgtgcagg
     5041 acatcgcagt agggcttttg cagaccgata ttgaagccga ggccgatcct gctgttcttt
     5101 ggctgggcag tggttgtctc ctgggccggt gcaatcgcag tgaaaatcag cagcacggcg
     5161 agggtgacga caatgatact gcgtttcata agttctcccc tcatataacc ttgaggaagg
     5221 tgttcagagt ttgttgattc tgagctgatt aggatagatc cgatgccgat accaggttgc
     5281 ccaccattca ggaaaactta tgcaaaaact gtaccactag caggcgtatg tgaatactat
     5341 atcgtaaccg gccgcaagac gccggctaaa tatatcttta gtgattgata ttatttaatt
     5401 aataaagccg cttcaggcgg ataatagccc ttccgggggc cttcatcccg gccacaaccg
     5461 cgctggcgtc tttccgctca ttcttgtatc agaacgctac agatgtccat tccaaatcgc
     5521 cacttaactg ctgatttggc gctgtttaac gcacccaaag ttattaataa attcttggat
     5581 tgtcaagtgc tttgttcact ttttggacag caattagcgc accaatgcca ttttgtgtgg
     5641 cttgaactgg ccaattccgc tgatcgcaca aaggtagatg ccggatggca gcggccggcc
     5701 ggagctgtca cggccgtccc agaactggca gttgagtccc ctggggaggc tgcgcatgat
     5761 ccgggaatag accacctcgc ctctttcgct cagaatatgg agcgccgcct cgctcacggc
     5821 ctcatggctg taaaattgga tctgcaagcg ctcatcctgc tccggccgga agggcgaggg
     5881 cgaaatcatc accactccat cctcgtgctc cgtctgatgg gcactcggcg caaagcggaa
     5941 ggtgtaggcg gcctttttct gggaggagcc tgcagagggc cactggatgt tgacgacggc
     6001 aaggcgcagc tcgcactcca tcgagaccgg accgaaagta agcggggtct cgccggcgat
     6061 ggtgaaatgg cgggactcat agccggcttc gtggatgacc agggtataga gccactgcgc
     6121 agagggatcg aggacgggcg taatcgtata gctcccccct gcagcgacag acagggtgta
     6181 ataatggctt tccagctggg agcaggagtt aagaatggtg cgttctccgc tgagggtgag
     6241 tttttcgacc ggggtgatct cgggaaaact gggactgtcg ggataataga gcaggctgtc
     6301 ggcatagtcc gctgtgaagg cgttccagat ggtgaaagtg gccagcgagc ggccaagatc
     6361 gttgccatag ctgcgcaggg cccgatcgag ggcatccagg ggttcctggt cgcgcagttc
     6421 ctcccacatc ctcctgatca gggcatagcc atggctcttg tggagcatgg tcatgtagag
     6481 tccaagcccg taggtgatgt tggaaaagag gtgaaaagga cggttgggcg aggcgaagag
     6541 cgagcgcaca tactggaaat agtcattgac ctcactgtag gcgacatcct cgagccaggt
     6601 cgaggaggcc tcgaagagga agcgggaatc catgcgtgtc gagggaaaga agcgataggc
     6661 gccttgcagc atatggaaga actcatgggc cgcggtgaca tggagggcgt cgattccctt
     6721 ggtctcatag ccagcgccgc tgaagcgatt gtcaatgacg atatagctgg tgtagcgctg
     6781 gccgtcggag ccctcgacgg gattctcata gcgcgtctct ccgtaggccg aaaaacgcat
     6841 gacgtagata tccagctccg gtccgtcgat gccatcgtca gagggtggtg gtgtaaagcc
     6901 catggtatgg acgaggagat cgtagacata gtcaaaggag cggccggcct cggcgatgaa
     6961 atccgcggcg gccgagtctg agccgctttc agcataatgg atgcggaaat ggccggccgg
     7021 actggtgacc gacaggggca atcggcccga ggtggggcgg gcgagaagtt gttccagcag
     7081 ggatctctcc tcaccggcga gtgttggcag cagcgcccgg acttccgctg taatcagcga
     7141 ggaacagtgc aggggatccc ggccggcggt cttatagcgc aggggcagcc ggtcgggatc
     7201 ttcggccgcg atgagctgat aggcgaggaa ctcccctctg gagatcgcac cggcctccat
     7261 ctcttgctga agcaaggttt gcagctgctg cgcggcggaa gcgctggcgg cccagatagt
     7321 caggatgatg agggcaatat gggagcgcat gatcatttca gtttttgaga cggaaggcca
     7381 gcctgatggg cacacccatg aaaccgaact gggcccgcag ctggttttcc agaaagttgc
     7441 ggtaattctg cttgataccg cggggttcat tggtgaaaat aataaagagg ggcggattga
     7501 ccttcgcctg ggtaatatag ttgagcttga cccattttgt tccataggcg ggcgggtgat
     7561 ttttctccac cacggcctgc aggaagcggt tcagctccgg ggtcggcacc gagcgctggc
     7621 attcgtcata gactgcgagg gccaggtcga gcagcttgaa gacgcgctgt ttttcgaggg
     7681 cggaaatgaa gagcatgggg ataaaggcaa gatccctgag ggcctccctg atgtcggtct
     7741 cgaagttctt cgcggtctgg ttgtccttct cgacggcatc ccatttattg acgccgagga
     7801 cgatcccctt gccggcctgc gctgccgcct ggagaatggt cttgtcctga tcggcgatgg
     7861 gctgggaggc gtcaatcagg accacggcaa tgtcacagcg ttcgagggcg tggtagctgc
     7921 gcaccgtgct gaaatactcg acattttcct tgacccgggc gcgtttgcgc aaccccgccg
     7981 tatcgatgag aacgagctgg cgctccttgt agcggagccg ggtgtcgaca gcatcgcggg
     8041 tggtgccggg caggggggtg acgatctgtt tatcggtgcc gagcagggcg ttgacatagg
     8101 aggatttgcc cacattgggc cggccgagga tggcgatatg caccgcctcc tccttttccc
     8161 tctcctccac cgccggggcc tccttgcgag gcagcaaggc ggtgagggca tcaagaaaat
     8221 cccccatgct gcgtccgctc attgccgaga tgacaaaagg atcgccgagg ccaaggcggt
     8281 agaattcgcc catatcggct tcgcggtact cgttgtcgac cttgttgatc gcgacgatga
     8341 cgcgggactg aacacgctgc agcatccggg cgatctcctc atccatggcc gtgatgccag
     8401 tggtgacgtc ggtgacaaag acgatgacat cggcctcttc aatggcataa tggacctgct
     8461 cgcggatggc ctgctcaaaa atatcccggc tgcccgcgac atagccgccg gtgtcgatca
     8521 ggtcaaaagc gaagccggac cattcagctg gctgatactt gcgatcgcgg gtgacgccgg
     8581 gctgatcatc gacgatggct tcccgcttgc ggatgatgcg attgaaaagg gtggatttgc
     8641 cgacgttggg ccggccgacg agtgcgacaa caggtttctt cttcataatt tcttcagata
     8701 ggtgaataat tctgtaaaac cggactgtgg cgccaatacg ggtgcatcca gtgctcggga
     8761 gagcccatcg agatcgagat catcgagaaa gagcccgtcg ccgttgcggc agttgggcgg
     8821 aatcatgatc agatcagccc tctccatgcc gcgcagttgg ccgataatat cccccccggt
     8881 gatcaggccc gaaaccgtga ctttaccccc gaaaaacctg ttcttgaccg cgaccacctc
     8941 ggcctgcagg ccgctgatgg tgttgaggac gggcaggacg cgggcggtca ggatcggggc
     9001 agccagttct ccgctcacta tgctcacccg caccggtttg acctcgcgcg gaaagcggta
     9061 tttctgccgg gcgaatctgt ccagaaagga gcgggtcatg ccgaccccgt tttcgatctg
     9121 gggaaagccg tcgtaccgcg cagcggaggg cagcggggcg ccggcgagaa ggtagaattc
     9181 atccgccaga tagacaaaag caatgcccag gcggctgcgg aactcctttt cccagcgctt
     9241 ttcactcaca accaggcctg ccgcctccgc cggtgtgacc gtgcgcaggg gggtaagctc
     9301 ctcacgatgg ccggtgaggc cgaggggaac tacggccaca gactggacgg ccggaaaacg
     9361 agcggcgaga tcggccagag tcttttcgag atgttcgccg tcgttccact ccgggcagag
     9421 cacaatctgg gcgtgaagtt cgatgccgcc ccgggccaaa aaatcgatct tttcgagcaa
     9481 gcggtcactg cggcgaagtc cgagcatacg cttgcgcaga tcgtcctctg tgctgtgcac
     9541 cgagacatac agcggcgaga ggcgctgttc gacgatgcgc tcgagatgac tgcgcgagag
     9601 gttggtgagg gtgacatagt tgccgtagag aaacgagagg cggtaatcct catccttgaa
     9661 atagagcgac ttgcgcatgc ctgggggatt ctggtcgaca aagcaaaaaa cgcatttgtt
     9721 gccgcaggag tggtattcca tatcggcgaa ttccactccg agccagccgc tttcctccgg
     9781 tgccagattc actttccatt cgtgtccacc gcggcggagg tgcaggcagt agctctcctc
     9841 ggctgagtag aactggaaat cgatgggatc gcgtatctca tggctgtcga tggcgatgat
     9901 ctcatcgccc ggttgcaggc cgagcctggc cgccggtgta tccggataaa cgacgctaat
     9961 tttcatt
//
LOCUS       DAOPWU010000101         9965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6826_length_9965_cov_4.213724, whole genome shotgun sequence.
ACCESSION   DAOPWU010000101 DAOPWU010000000
VERSION     DAOPWU010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6826_length_9965_cov_4.213724"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1015
                     /locus_tag="PKM23_06910"
     CDS             134..1015
                     /locus_tag="PKM23_06910"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNY91241.1"
                     /translation="MKRILSCAALTALALLHFSTAEAQVSELKFSHKLHSEAVGASCT
                     DCHGAAESALAADNLLPDMESCYNCHDREAECSLCHSDVDNAKAEPRISSYIAKFPHA
                     RHISDKMTCESCHAGIAASESVAGAHLPGMAKCSSCHDDLAKPGYCYDCHAGGEKLEP
                     ASHTLDWPKAHGVRMQTETEECKLCHTDDQCIVCHKKGNLDRKAHPLNFINNHGLAAK
                     GNKEQCLTCHEDEGYCVGCHQSMMVMPRSHARAAWSNPTNGGAHARAARADMDECTVC
                     HSDAASQPICIQCHTAQ"
     gene            1148..2683
                     /locus_tag="PKM23_06915"
     CDS             1148..2683
                     /locus_tag="PKM23_06915"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNY91242.1"
                     /translation="MTRNLIYFLVAVAGAAVLAIELLGTRVLGPFYGVSLYLWSALIV
                     VTLLSLSCGYYLGGRWADRGATLHRLAGLLAVAGLSLLLVPLIKRPLLLLLQPLGLRA
                     AVLLGALLLFAPSLTLLGMVTPYALKLKTERLDKLGRTAGSLSALSTLASVIAALLTG
                     YWLIPAMGINRLTVAIAALLLLSAAIAIWGERRRLRQAALLLIAGVGISLLQMQLQPG
                     EEPGLLFAGQSPYGEIRVLEWQGARYLLIDGAVHTAVSSADLRTEMRYVVALDIVRNF
                     FDRTGEMLLIGLGGGCVARNWAEAGWRVKAVEIDPVVARMAASCFGLRPEEAEVHIID
                     GRNYLNRTGPPFEVIILDAFGSSSIPWHLTTAEVFHLIRARLTDDGLLAVNVEVLGWE
                     DPLLQSLALTLRSSFSHVIALPTAEPPDALGNVLLLAANRPLEFPEEWLGRPIDFLED
                     AYAHWTVVQRNHAWDNRYAPATAAAVLLTDDRNPSDLWSERINFAVRRRLPELFPGRP
                     LIW"
     gene            complement(2806..3918)
                     /locus_tag="PKM23_06920"
     CDS             complement(2806..3918)
                     /locus_tag="PKM23_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91243.1"
                     /translation="MTRKLKFVLIGAAVLLILALMLGCGEKSAAPTAVEPGAQTSAWT
                     MDNPLLRTVAAVQERFTPELMKNPEVVGTAVTLSEEGKPVILVMTKSAALQKGPGGLP
                     YELEGVPLLVEVTGEIKALKTTTPTVSHTAKQTAPIQLGTSGGWRYDLANGYCCGGTL
                     GSLIQIGGSKYILSNYHVLEADIVVGGNSRIATTGDDVIQPGLIDVNCTATSAQNVAT
                     LVKKSALPGNNVDCAIAQIIPGMVRDDGAILEIGVLSSSTVAAAVSQAVKKSGRTTGL
                     TRSTVSALNGTVSITYENECAGGTAFTRTFTGQIIIANKRSKFLDSGDSGSLMVQDLT
                     TNPKAIGLLFAGSTSTAIANPINEVLTFLGGSMVGL"
     gene            4128..4292
                     /locus_tag="PKM23_06925"
     CDS             4128..4292
                     /locus_tag="PKM23_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91244.1"
                     /translation="MVMLSLASGSFSAGTHLLNWDDRTDGGELAGAGTYFYRLESEGG
                     ILTRKMIFLK"
     gene            complement(4396..5829)
                     /locus_tag="PKM23_06930"
     CDS             complement(4396..5829)
                     /locus_tag="PKM23_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNY91245.1"
                     /translation="MQKKLWIFAGLLFLLTPLLLAGETGTGSEVNLGQVLPVWSIIPF
                     AGILLSIALFPLFAPKFWHHHYGKVSAFWALAVAIPFILVYGHPAVHEILHIILIDYL
                     PFIILLLALFTVAGGILVEGTLKGTPALNSLLLLIGTVIASWIGTTGASMLLIRPVIR
                     ANAGRRSKIHVIVFFIFLVSNIGGSLTPLGDPPLFLGFLHGVPFFWTFQLLPETLFVA
                     GLVLAIFLIMDSYLYNRERKAGLLTEASEEKTPVRIRGAHNLIFLAGIVGAVLMSGTY
                     HAGEVTVLGIHLGLANLLRDFILLILAGLSWATTAKELRRKNEFTWGPIVEVALLFAG
                     IFITIIPALAMLRAGTQGALAFIINAVKEPWHYFWITGALSSFLDNAPTYLTFLNTAL
                     GNFFAGMPEKEAIAALIADKGIFLKAISTGAVYMGANTYIGNAPNFMVRSIAEEQGIK
                     MPSFFGYMGYSLLILIPVFILTNFIFF"
     gene            complement(6207..>9965)
                     /locus_tag="PKM23_06935"
     CDS             complement(6207..>9965)
                     /locus_tag="PKM23_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY91246.1"
                     /translation="VMDRLTDGQIGKVGQPLDKPIKVTIKDKYGNGVPGTPVLFHVSG
                     IDSLRGHIGGLSETSVATDLDGSASVVWNLGKRPGSKNNAMEVSARYNNTNLSGSPAI
                     FYASATVGDPKLILMVSDTSRFTGITTNMLPEPLKVRITDEFKNPIANHSVVFTVMSS
                     VAADGGSLDGATNKTVTKLTDSNGLASVQFYLGRNAGYKINRVEADAEYNGLKLTGAP
                     LPFLISGAPSNAENLLLSTGDGQIGTVGKFLTNELAVMARDVYNNPVKGHPIQFRILV
                     GASDHAALGADTLLTKVVETGSDGIARVKWRLGRTAGADRNVVEVTSTNGTTPLKNSP
                     IRFTANALPDITDGVRSKIAAVDAAVSADGISRATIKVSLRDKYENPVAGKYVTLLSS
                     DLTTIITQPLNTTDVNGDAVGFAASTKAGSKWIRARDVNNNVSIADSVRVTFLPLAAY
                     EIARTNSQDGDGQTRNVGTALPLPLRVVVRDRFGNPIAGHPVTFMPTQGGGAMIDPQV
                     ISTDSAGIAQARFKLGSAAGINVVEARAVKSDGSGQALSNSPVRFTESAVVPSPSRLV
                     IIAGDQQSAAPQQPLPQPFKVQLEDINGWPVATVQVKFSPLVNNGAITSTNPVLTDMY
                     GQATAQAVAGTGNGTSLFSAGLPNYSAISAVTFTAYTLPGAASKIVYQMGGDQTGTVG
                     RTLYTPLAVRIEDSFGNTVSGVPVTFLVVDDGTIEGKGTLDNGTTMMTVTSNPQGIAS
                     ANYTLGTRTGLNKVRASAVNLNPPFVEFEAHGQADYPYTMEKIESEPLHGEVGKRMVY
                     PIQVLVKDRYGNPASGGTINFVVVPGSGSIDGPNLVTSNSSGIARAYWILGKQGRNEA
                     LATASLPSGSPTVTFEARGDIEPYPELPVASEYTLYEGQQLCFPVLATDRDGDQLYYS
                     ASNLPEGATFEVDGSNIYHFCWTPTYEQGGQTYYPRFTVLDSDGGIDIDSVKIVVTNE
                     NRAPRLVSWEPASESFKIPPLASQTFYIQVEDPDNDPIYYTWRLNGQVVGSSSSYTFD
                     SRYYPLGNNYMIMVEYGDATHTATLVWSIVTAVELKSFTCASVAYQGITLSWQTASET
                     DNMGFNVLRSRTEKGTYEKINPDLISPRMDGQYNWVDKSAKAGERWYYKLEDISRSGL
                     ATQHGPVSADMPVPTRFELAQNYPNPFNPVTNIRYQLPADGKVTLQIFNTNGQLIRTL
                     VDGEVPAGYHQIVWDSCNDSGSRVVSGIYYYRIIANGTSVTKKMALLK"
BASE COUNT         1890 a         2975 c         3131 g         1969 t
ORIGIN      
        1 gggcagcctg cacgccggga cacgctggaa tgtgatcgcc gaatatcagt ggctgaccaa
       61 ccgctttgcc gagagcgatg cgcgtttcct gaaccatatc acataccgct ggtaagcgaa
      121 gaggagcccc gccatgaaaa gaattctcag ctgcgccgcc ctgaccgctc tggccctcct
      181 ccatttctca accgcggagg cccaggtttc agaactcaaa ttctcgcaca agctgcacag
      241 tgaagccgtc ggcgcctcct gcaccgactg tcacggtgcg gcggagagcg ccctggcagc
      301 ggacaacctc ctgcccgaca tggagagctg ctacaactgt cacgaccgcg aggccgaatg
      361 cagcctctgc cacagcgatg ttgacaacgc caaggctgaa ccgcgcatca gcagctacat
      421 cgccaagttt ccccacgccc gccacatctc ggacaagatg acctgcgaat cctgccacgc
      481 cggcatcgct gccagcgaga gcgtcgccgg ggcgcatctg ccgggcatgg ccaaatgctc
      541 gagctgtcac gatgatcttg ccaaaccggg ctactgctac gactgccatg ccgggggcga
      601 gaagcttgag ccggcctccc acaccctcga ctggcccaaa gcccacgggg tgcgcatgca
      661 gaccgaaacc gaagagtgca agctctgcca caccgacgac caatgcatcg tttgccacaa
      721 aaagggcaat ctcgaccgca aggcccatcc cctcaatttt atcaacaacc acggcctcgc
      781 cgccaagggc aacaaggagc agtgcctgac ctgccacgag gatgagggct actgcgtggg
      841 ctgccaccag agcatgatgg tgatgccgcg ctcgcacgcc cgcgccgcct ggtccaatcc
      901 gacgaacggc ggggcgcatg cccgcgccgc ccgcgccgac atggacgagt gcaccgtctg
      961 tcattccgac gctgcaagcc agccgatctg catccagtgc catacagcgc agtaaccagc
     1021 tctggtaacg aagccggagt gccggaagaa aaagttctcc cctgcgcaag cctgcgcgaa
     1081 ccgcgtcacc ggctgtcatg ccaatgaccg gtaaaaaaag atctgagacc actggaatca
     1141 ctggataatg actagaaacc tcatctactt cctcgtcgcg gtggcgggtg cggccgtgct
     1201 cgccatcgaa ctgctcggca cccgcgtcct cggtcccttt tacggtgtca gcctctatct
     1261 ctggtccgcc ctcatcgtcg tcaccctgct ctcgctgagc tgtggctatt accttggcgg
     1321 gcgctgggcg gatcgcggcg ccaccctgca caggctcgcg ggactgctgg ccgtagccgg
     1381 cttgtcgctg ctgctggtgc cgctcatcaa aagaccgctg ctgcttcttc ttcagcccct
     1441 gggtttgcgg gcggcggtgc ttctgggcgc cctgctgctt ttcgccccct ccctcaccct
     1501 gctgggcatg gtcaccccct atgccctcaa gctcaagacg gaacggttgg acaagctggg
     1561 acggactgcc ggttcactct ccgccctctc caccctggcc agtgtcatcg cggccctgct
     1621 caccggctac tggctcatcc cggcgatggg aatcaaccgc ctgactgtgg caatcgccgc
     1681 cctgctcctt ctgagcgcgg ccatcgccat ttggggcgaa cggcgccgtc tgcgccaggc
     1741 ggcgctcctg ctgatcgccg gtgtgggaat cagcctgctg cagatgcagc tgcagcccgg
     1801 ggaagagccc ggcctgcttt ttgccggtca aagtccgtac ggcgagatcc gcgtcctgga
     1861 gtggcagggc gcgcgctatc tgctcatcga cggggcggtc cacacggcag tttcgtccgc
     1921 cgatctccgc accgagatgc gctatgtggt ggccctggat attgtgcgta acttttttga
     1981 ccgtacggga gagatgctgc tgatcggcct gggcgggggc tgcgtggcgc gaaattgggc
     2041 ggaagcggga tggcgcgtca aggcggtcga gatcgatccg gtggtggcac gcatggccgc
     2101 aagctgtttc gggctgcgcc cggaagaggc ggaagtacac atcatcgacg gccgcaacta
     2161 tctgaaccgg accgggccgc ccttcgaggt catcatcctc gatgccttcg gctccagctc
     2221 catcccctgg catctcacca ccgccgaggt ctttcatctc atccgcgcac ggctgaccga
     2281 cgatggcctg ctcgctgtca atgtcgaggt cctgggctgg gaggatcccc tgctgcagag
     2341 cctggccctt accctgcgca gcagcttcag ccatgtcatc gccctgccaa cggccgagcc
     2401 cccggacgcc ctcggcaatg tcctgctcct cgccgcaaac cgcccgctcg aattccccga
     2461 ggagtggctc ggccggccaa tcgactttct ggaggatgcc tacgcccact ggacggtggt
     2521 tcagcgcaat cacgcctggg acaaccgcta tgcgccagcc acggccgcgg cggtgctgct
     2581 gaccgacgac cgcaatccct ccgacctttg gtctgagcgg atcaattttg ccgtccgccg
     2641 ccgtctcccc gagctcttcc ccggccgtcc gctcatctgg taattccgcg cccctttccc
     2701 ccgctatcaa ccaacttgtg cgtggcataa aaaaaggcac ccgcgccttt cggtgcgggt
     2761 gcctttgata acctgtctgg ggcggatgac agccgctgca gtggattaca gacccaccat
     2821 cgagccgcct aaaaaggtga gtacttcatt aatcgggttg gcaatcgcgg tggaggtgct
     2881 gccggcgaag agcagaccaa tggctttggg attggtggtc aaatcctgca ccatcagcga
     2941 gccggaatcg ccgctgtcca ggaacttgct tctcttgttg gcgatgatga tctggccggt
     3001 gaaggttctg gtgaaagcag tgccaccagc gcactcgttt tcataggtga tcgacacggt
     3061 accattgagt gccgacaccg tgctgcgggt caaaccggtg gtgcggccgc tctttttcac
     3121 cgcctggctg acagcagcgg cgacagtgga agaggagagg acgccaatct cgaggatggc
     3181 accgtcatcg cgcaccatgc cgggtatgat ctgggcgatg gcgcagtcga cgttgttgcc
     3241 ggggagggcg gatttcttaa ccagggtggc gacgttctgc gccgaggtcg ctgtgcagtt
     3301 gacatcgatc aggcccggct ggatcacatc atcaccggtc gttgcgatcc tgctgttgcc
     3361 gccaacgaca atgtccgcct cgagcacatg atagttgctg aggatgtact tgctcccgcc
     3421 gatctggatc agggagccga gcgtgccgcc gcagcagtag ccattggcga ggtcataccg
     3481 ccatccgccc gaggtgccga gctggatcgg tgccgtctgc ttcgcggtat ggcttaccgt
     3541 tggggtggtg gttttcaagg ctttgatctc gccggtcacc tcgacgagca agggaacgcc
     3601 ctccaactca tagggcaggc cgcccggtcc tttttgcagc gcagcacttt tggtcatcac
     3661 gagaataacc ggcttgccct cttcggagag cgtcaccgcg gtgccgacga cttcaggatt
     3721 tttcatcaat tccggggtga aacgttcctg caccgcggcc accgtcctga gcaagggatt
     3781 gtccatggtc catgcgctgg tctgcgcacc tggctcaacc gcggtgggcg cggccgactt
     3841 ttctccacag cccaacatca gcgccagaat cagcagcaca gcggcgccaa tcagcacaaa
     3901 tttcagtttt ctcgtcatcg gatcgacctc ctcgcgctag ggattccccc tcctctgtaa
     3961 atttgacaat aatagttatg ttccgctcca attgcaagtg ttttttaatc tttttttcac
     4021 tcaggcggcc atcattctca aaaatcgacc agcagcattc tgcggcaact ccctcataaa
     4081 taaacagact aactacttga ctacgatgat cgcatcaaaa atcttttatg gtaatgcttt
     4141 cactcgccag tggttccttc tccgccggca cacacctgct caactgggac gaccgcaccg
     4201 atggcggcga actcgccggt gcgggcacct atttttaccg ccttgagagc gaggggggta
     4261 tcctcacccg caagatgata ttcctcaagt aagcgaaatc ttgccttaaa accgggccgg
     4321 aacgtatcag cgttccggct ttttttgcag cataaaaaag ggcgccacgc ggcgccctcg
     4381 cagtcaaacc tagttctaga aaaatatgaa gttggtgagg ataaagaccg gaatgaggat
     4441 gagcagggag tagcccatgt aaccgaaaaa gctcggcatc ttgatcccct gctcctcggc
     4501 gatggagcgg accataaagt tgggggcatt gccgatgtag gtgttggcac ccatatagac
     4561 ggcgccggtg gagatggcct tgagaaagat ccccttgtcg gcgatgaggg cggcgatggc
     4621 ttccttttcc ggcatgccgg cgaaaaagtt gccgagggcg gtattgagga aggtcagata
     4681 ggtcggggcg ttgtcgagaa aactggagag ggctccggtg atccagaaat agtgccaggg
     4741 ctccttgacg gcgttgatga tgaaagcgag ggcgccctgg gtgccggcgc gcagcatggc
     4801 cagggccgga atgatggtga taaagatgcc ggcgaagagc aaggccacct cgacgatcgg
     4861 cccccaggtg aactcgtttt tccggcgcag ctcctttgcg gtcgtcgccc aggaaagtcc
     4921 ggccagtatc aacaggataa aatccctgag caggttggcc aggccgagat ggatacccag
     4981 cacagttacc tcccccgcat ggtaggtgcc gctcatcagc acggcgccga cgatgccggc
     5041 caggaaaatg agattgtggg cgccgcggat gcgcaccggc gtcttttcct cgctcgcctc
     5101 ggtcagaaga ccggctttgc gctcacggtt gtagagatag ctgtccataa ttaaaaagat
     5161 cgccagcacc aggccagcca cgaagagggt ttcgggaagg agctgaaagg tccagaaaaa
     5221 gggcacgccg tggagaaaac cgagaaagag gggcggatcg cccagggggg tcagcgaccc
     5281 gccgatattg ctgaccagaa aaatgaaaaa gacgatgacg tgaatcttgc tccggcgccc
     5341 ggcattggcc ctgatcaccg gacggatgag cagcatcgag gccccggtcg tgccgatcca
     5401 tgaggcgatc accgtgccga tcagcagcag caggctgttg agggcgggcg tgcccttgag
     5461 ggtgccctcg accaggatcc ctccggccac ggtgaaaagg gcgagcagaa ggatgatgaa
     5521 ggggagataa tcgatgagaa tgatgtgcag aatttcatgc acggccggat ggccgtagac
     5581 gaggatgaag gggatcgcca cagcgagggc ccagaaggcc gatacttttc cgtaatggtg
     5641 gtgccagaat ttgggggcga agagcgggaa cagggcgatg gagaggagaa tgccggcgaa
     5701 ggggatgatg ctccagaccg gaagcacctg gcccagattc acttcactcc cggtcccggt
     5761 ctcgcccgcc aggagcaggg gcgtaagaag aaagagcagt cccgcgaaaa tccacagctt
     5821 tttttgcata gcctaccgat cctttgttag agcttccggc ctgaaggatg cctggccaaa
     5881 tcccgccgta ccgataatta tccgtaaaat tatcaaaaat atttcccaac atcaacaaaa
     5941 aataacatcc agggtacccc ccgaaatccg ggtcccaccg tgacgtttta cgctgcgtgc
     6001 actcgaaacc tggctcccac cggactcttt gcgccgcata gcccgattgc tccgaagtag
     6061 ttccagcata ctcgcgggct gtgccctggt cagcgaggca actttttttt taattacatc
     6121 cggataggaa cgaaattttg gggggcagca taaaaaaaag ccgccccgga agggcggctt
     6181 ttgattgata caggatgaca ccgggcttat ttgagcaggg ccatcttttt ggtcacgctc
     6241 gtgccgttgg cgatgatgcg gtaataatag atccccgaaa ccacccggct gccggaatcg
     6301 ttgcagctgt cccagacgat ctggtgatag cctgcaggaa cctcgccgtc gaccagggtg
     6361 cggatgagct ggccgttggt gttaaagatc tgcagggtca ccttcccgtc cgccggcagc
     6421 tggtagcgga tattggtcac cgggttgaag ggattgggat aattctgggc cagctcgaag
     6481 cgggtcggca ccggcatgtc ggcgctcacc ggcccgtgct gggtcgccag gccgctccgg
     6541 ctgatgtcct cgagcttgta gtaccagcgc tctcccgcct tcgcgctctt gtccacccag
     6601 ttgtactggc cgtccatgcg cggagaaatc aggtcgggat tgatcttctc ataggtgccc
     6661 ttctcggtgc ggctgcgcag cacgttgaag cccatgttgt cggtctcgct ggccgtctgc
     6721 caggagaggg tgatgccctg gtaggcgacg ctcgcacagg tgaagctctt gagttcgacg
     6781 gcggttacta tggaccagac caaggtcgcg gtatgggtcg catcgccgta ctcgaccatg
     6841 atcatatagt tgttgcccag cggatagtag cgcgagtcga aagtatatga tgaagaactc
     6901 ccgaccacct gtccgttgag ccgccaggtg taatagatgg ggtcgttatc cggatcctcg
     6961 acctgaatat agaaggtctg ggaagctagc ggcggaattt tgaaagattc ggaggccggc
     7021 tcccaggaga ccagccgcgg tgcgcggttc tcgttggtga cgacaatctt gaccgaatcg
     7081 atatcgatgc cgccgtcgct gtccaggacg gtgaagcgcg gatagtaggt ctggccgccc
     7141 tgctcgtagg tcggcgtcca gcagaagtgg tagatattgc tgccatcgac ctcgaaggtg
     7201 gccccttccg gcaggttgct ggcgctgtag tagagctggt cgccgtccct gtcggtggcg
     7261 agcaccggga agcagagctg ctggccttcg tagagggtgt actcgctcgc gacgggcagt
     7321 tccggatagg gctcgatatc gccgcgggcc tcgaaggtca cggtcggcga gccgctcggc
     7381 aggctggccg tggccagggc ttcattcctg ccctgcttgc cgagaatcca gtaggctctg
     7441 gcgatgccgc tgctgttgga ggtcaccaga ttgggtccgt cgatgctgcc gctgccgggc
     7501 acgaccacga aattaatggt gccgccgctc gccggattgc cgtagcggtc cttgaccagg
     7561 acctgaatcg ggtagaccat gcgcttgccg acctcgccgt gcaggggctc ggactcgatc
     7621 ttctccatgg tgtagggata gtcggcttga ccatgggcct cgaactcgac gaagggcgga
     7681 ttgaggttga ccgccgaggc gcgcaccttg ttgagaccgg tgcgggtgcc gagggtatag
     7741 ttggccgagg cgatgccctg gggattggag gtgacggtca tcatggtggt gccgttgtcg
     7801 agcgtgccct tgccttcaat cgtgccgtca tcgacgacca ggaaggtcac tggcacaccg
     7861 gagacggtgt tgccaaaact gtcctcgatc cggaccgcca gtggggtata gagggtgcgc
     7921 ccgaccgtgc cggtctgatc gccgcccatc tgatagacga tcttgctcgc cgcgcccggc
     7981 agggtatagg cagtgaaggt gacggcgctg atagcgctgt agttgggcag accggcgctg
     8041 aagaggctgg tgccgttgcc ggtgccggcg acggcctggg ctgtggcctg accatacata
     8101 tccgtcagca ccgggttagt gctggtgatg gcgccgttgt tgaccagggg gctgaacttg
     8161 acctggacag tggccaccgg ccagccgttg atgtcctcca gctgcacctt gaagggctgg
     8221 ggcaggggct gctggggcgc agccgactgc tggtcacccg caatgatcac aagcctggag
     8281 ggcgaaggca caacggcgga ttcggtgaag cgcaccggcg agttgctgag ggcctggccg
     8341 ctgccgtcgc tcttgactgc gcgggcttcg acgacattga tgcccgctgc actgcccagc
     8401 ttgaaacgcg cctgggcgat accggcgctg tcggtggaga tcacctgggg atcgatcatc
     8461 gcccctccac cctgggtggg catgaaggtg acgggatggc cggcgatcgg gttgccgaag
     8521 cggtcgcgca ccaccacacg caggggcagg ggcagggcgg tgccgacatt gcgggtctgg
     8581 ccgtcaccat cctggctgtt cgtgcgcgcg atctcgtagg ccgccagggg caggaaggtc
     8641 accctgacgc tgtcggcgat gctgacattg ttgttgacgt cgcgggccct gatccacttc
     8701 gaacccgcct tggtcgaggc ggcaaacccc accgcatcgc cgttgacatc cgtggtattg
     8761 agcggttggg tgatgatggt ggtcaggtca ctggagagca gggtgacata cttgccggcc
     8821 accggattct cgtatttgtc gcgcagggag accttgatcg tcgctctgga gataccgtcg
     8881 gcactcaccg ccgcatccac agccgcgatt ttggagcgca ccccgtcggt gatgtccggc
     8941 agggcattgg cggtgaagcg gatgggtgaa tttttcaacg gagtggtgcc gttggtcgat
     9001 gtgacctcga caacattgcg gtccgcgccg gcggtgcggc ccagacgcca tttcacccgg
     9061 gcgatgccgt cgctgccggt ttcgaccacc ttggtgagca gggtgtcagc gccgagggcg
     9121 gcatgatcag aggccccgac caggatacgg aactgaatcg gatggccctt gacgggattg
     9181 ttgtagacat cacgggccat gacggccagt tcgttggtga ggaacttgcc gaccgttccg
     9241 atctggccgt cgccggtgga gagcagcagg ttctcggcat tgctgggcgc accggaaatc
     9301 aggaagggca ggggcgcgcc ggtcagcttg aggccgttgt actcggcatc cgcctcaacg
     9361 cggttgatct tgtaaccggc gttgcggcca agatagaact gcaccgaggc cagaccgttg
     9421 ctgtcggtga gcttggtcac ggtcttgttg gtggccccgt ccagggagcc gccgtcggcg
     9481 gcgaccgagg acatcactgt gaaaacgacc gaatggttgg cgatcgggtt tttgaactca
     9541 tcggtgatgc gcaccttgag cggttcgggc agcatattgg tggtgatgcc cgtgaaacgg
     9601 ctggtatcgc tcaccatcag gatcagtttc ggatcgccga ctgtcgcact ggcgtagaaa
     9661 atggcgggcg agccggagag attggtgttg ttgtagcgcg cactcacctc catggcgttg
     9721 ttcttgctgc ccgggcgttt gccgaggttc cagaccacgc tcgcactgcc gtcgagatcg
     9781 gtggccaccg aggtttcgga gaggccgccg atatggccgc gcaggctgtc gatgccgctg
     9841 acgtggaaga ggaccggtgt gccgggcacg ccgttgccgt acttgtcctt gatcgtcacc
     9901 ttgatcggtt tgtccagggg ctggccgacc ttgccaattt ggccgtcggt caaccggtcc
     9961 atgac
//
LOCUS       DAOPWU010000102         9897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6885_length_9897_cov_4.638691, whole genome shotgun sequence.
ACCESSION   DAOPWU010000102 DAOPWU010000000
VERSION     DAOPWU010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6885_length_9897_cov_4.638691"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PKM23_06940"
     CDS             <1..514
                     /locus_tag="PKM23_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91247.1"
                     /translation="LEREPEALVMAGTVMTSEQADRVIEMGVAGIVCPDYFPEVVERC
                     VAADLLCIPGGISGVGAQLAQKARLYGCTIEELPLLYSYQYIHKLFPAITETVSFLGL
                     ASAWKGPYKGLQMVYTGGINRDNLAAAAAFDRSGIFCGSALTKAAPDRAGMRAEGEKW
                     LALLAEKSPE"
     gene            511..1245
                     /locus_tag="PKM23_06945"
     CDS             511..1245
                     /locus_tag="PKM23_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNY91248.1"
                     /translation="MSSPGAWSALIVDDEWLVREELKGLLAGYPEIRVEGEAGSVRQA
                     AEQLARRAFDVIFLDIQMPGALGFELLEQAEVTARIIFITAYDQYAIKAFEVNALDYL
                     LKPIQKERLGQSIARLSGRAPEALRHKPKLGYDDVAYVMVAGSLKFLQVAQIRCITAG
                     GNYSYLYYQDHRRELVAKSLQEWEEILPGEYFQRIHRSALVNFAFVERVEKCRNYSHL
                     VHVRGVEKPLVMSRRYSARLHHLLPL"
     gene            complement(1258..4419)
                     /locus_tag="PKM23_06950"
     CDS             complement(1258..4419)
                     /locus_tag="PKM23_06950"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNY91249.1"
                     /translation="MKPPSLILILIFFLALSPAGVQGAEAQPSNSQERELQFRNLTID
                     QGLAHSKVNCFLQDRQGFIWCGTNEGLNRYDGYDFVVYQQDPANPGGLSANLIRCLAE
                     DEQGYLWIGTEDGGLNRFDRDSGVFQRFGADSSAGLLLSGDNINAMLLDSSGCLWVGT
                     ENGLDRISRDRSRIASFFPYPGKAAAGANEILALHLDRGGLLWVGTNSGGLLSFDPSG
                     ESFTPYRHLRGDPHSLSDDEIHSICADREGDLWIGTAWGGLNRLERSTGRFSAIHPGA
                     GNPESTTIRALLDDGRGSLWIGNRSGLYRLERSSGVITSHTHDPNNPHSLVQNSVQAI
                     FQDRKGDLWIGTRGGISFLNTTNIPFVHYRADAASRRCLNSQEVYAIMEDRSGDLWFG
                     TESGGINRLDRRSGLFSYYTYEPGNPSCLSVNNIKALLQDRRGNLWIGTFNGGLNLLP
                     GTAGRRFTRYTHRTGDPASLANDNVLALLQDEAGEIWVGTYGGGLDRFNPRTGRFDHL
                     LTTWHDQGFSSVLSLLQDRAGHIWVGGSRTSVGRLDPASGDFRIIGLNDSGRDIQVRA
                     LLQDRKGMIWIGTVGAGLFRLDPDSEAVTAFSTRDGLPANVVHGLLEDESGCLWLSTS
                     DGLCRFDPETRRIKCFYKENGLQSNQFNYGAALKTRDGELFFGGINGATGFYPGAIRE
                     NTFIPPVVITQFALFNRPVEVNAPNRLLKQEISRTRRIDLSWRHTVFSFEFAALNYAL
                     SEQNQYAYIMEGFESEWNHVTNRRFVTYTNLDPGAYTFRVKAANNDGIWNEEGTAIHI
                     TISRPFWKTYWFTLLALAALLMLVKLFIDYIRQRRDLLKARSLASLSQLKLLRYQLNP
                     HFLFNALGSIRSMILISQEQAWDMVSALSEFLRYSLLNFNKAEALLDDEITAVTNYLN
                     IEKVRYRDSLQVAVSIDEKARQLVVPAFICQPLVENAIKYGMQTSPLPLQVTLNIRYR
                     DEVLAIDVSNSGRLKPLPDGAKTQSDGHGNSVENIRQRLEIMFREEQNFSLTEEQGMV
                     HARIRITSRGLKRKSLEEMGSKG"
     gene            4702..5073
                     /locus_tag="PKM23_06955"
     CDS             4702..5073
                     /locus_tag="PKM23_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY91250.1"
                     /translation="MKELRTLIIDDEWLIRLELRRMLQSMPNIRIVGEAATVAEAAQL
                     AESASPDLVFLDLQLGDGSGADFLNLAQGTFSLIFVTAFECNLPKVTNHPTLACLAKP
                     INKDKLYAVLQTLQKGEGVQV"
     gene            5070..8054
                     /locus_tag="PKM23_06960"
     CDS             5070..8054
                     /locus_tag="PKM23_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91251.1"
                     /translation="MRKTILFVILILLTSFSALLAQATITGTVADSAKGDPLIGANVY
                     LVGTALGSVTNIEGAYRIVGIPEGSYTLRISYIGYRTRNLRVQPKAGESLERNVRLVS
                     DIAKGISMDVTAQAVGQMAAINQQISAKTIVNVISEEKIQELPDANAAESIGRLPGVS
                     LLRSGGEANKVILRGLEDKFTVITVDGIKIPPTDATSRGVDLSTLSQSSLAGIELYKA
                     QTSDKDGDALAGSINLVTRKAPAEREIKATLKGGYNQLEEKANQYDASFHYGERFFGS
                     LLGVQLAGNIEQRIRSNERIDVDYNQSLNFGRDYFINDFTLEYTDELRKRDGASLLLD
                     FNTPDNGSIKLNNVYGRTRRDYFLSTRDYPSNGGGDQQGAPTFNFRDREQEIKTLTSA
                     LSGDNHLLGLNLRWGLSYAQSEADFPFDFQTIFVGLDGMKPSPMLRENPEQLIGYANK
                     NYNEAGLSWAYNRGQHNLDKERTAFLDVARPYTLSRLFSGEFKIGGKYKVKDRSNERF
                     EDFTPYYLGKWQPYEMLPDGTFRNKNFAGSAFADWASTAISGSGIAIDRFFKDPVTRS
                     IYGSYDLTPIIDRDRVREWYELNRYGVDVTKNIHEVWNNPLYEYDDYDITERVGAGYV
                     MNTLNLGRFATVIAGVRVEKEENDYLAGYMSRPSSGFPISPNAVRDTTSDASQTVVLP
                     NVNVALALFDFMKLRLAAFKALARPDFNMRLDRYIAGRGAEVGSNFELLVGNPDLKTA
                     EAWNFEVNPTFFSGTIGLISLSAYYKEIDDMYHMLNDFGTTGDSLLQRFGIGWPSQML
                     GTAYSLTLPYNSPKPTKVWGFEFEHQVNFNFLPGLLKNIVLSYNASIVRSEAYIWTAR
                     LDSVFYDPPGPIKGAWKKFNTLHEAKRKLENMPEFFGNIALGYDIGRFSGRVSLYHQG
                     EHNVSYSASGLSDVVTNAYTRVDLALRQGISKNISLFLNVNNLTDVEEGTSVRNRVFD
                     RNLFNMSEKYGTTADFGVIAEF"
     gene            8142..>9897
                     /locus_tag="PKM23_06965"
     CDS             8142..>9897
                     /locus_tag="PKM23_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91252.1"
                     /translation="MRFRVLAVLAIALLCSAAVVFAQETVVPITPSDGSAALMINVQI
                     AADTVGRGFRADRVYEFKRGEIYLQNATLTVPAGQTIRYRASEGDGPLPVIYLWPTGT
                     GSNPTRPPGWAITLAGGHARVSDLCMVGVYEYEPTSFDNMQGGLIQTNAIGGSIILDN
                     VILSNTNGNHVRTNNAATKVIATNCTFANMGNLVTSNFGAGKAFDLRDVQVDTFIVEN
                     CTFVNGQDRPIRHWNATSTQPIIYGRINHNTFVNGMGFHGLLSLGGVGEEMIITNNLF
                     IDAFALGEDSTDATRGAEWANTGEVYANGLNKITWIFTTPNDTTKWKVSNNFFAISDS
                     GQAFLTKFGFGPGNPLSAHIQSKLGAGAATAFTKIDLTPVNIPKLMTNLMRWYEDPAG
                     GNKTKDKANYVRARDDFDRRFVEYFRDTLDVSYATNLAAYTGGEAGFPVGDLNWFPDK
                     KAQWENQAAIPTIASIKVDANADFVPDLKDQVVTIQGVITTINYSNKLQYYMQDATGG
                     INLYSGAIEMALNMGDVVTVTGQLQHYNGLTEIVPAAAADVVVVGTQAVPEPLPTTAA
                     GLNEETEGMLVKVFRYRIV"
BASE COUNT         1894 a         3053 c         3043 g         1907 t
ORIGIN      
        1 ccttgagcgc gagcctgagg ccctggtcat ggccggcacg gtgatgacct cggaacaggc
       61 agaccgcgtt atcgaaatgg gtgtggcggg catcgtctgc ccggattatt ttcccgaggt
      121 ggtcgaacgc tgcgttgctg ctgacctgct ctgcatcccc ggcggcatca gcggggtggg
      181 cgcgcagctc gcgcaaaagg cgcgcctcta cggctgcacc atcgaggagc tgccgcttct
      241 ctattcctat cagtatatcc acaagctctt ccccgccatc accgaaaccg tctccttcct
      301 cggccttgcc tccgcctgga aaggccccta caaggggctg caaatggttt acaccggggg
      361 catcaaccgg gacaacctgg ctgccgccgc cgcattcgac cgcagcggca tcttttgcgg
      421 ctccgccctg accaaggcgg cgcccgatcg ggcgggcatg cgcgctgaag gggaaaaatg
      481 gctcgccctt ctggcggaga aaagtccgga atgagcagcc cgggagcatg gtccgccctc
      541 atcgtcgatg atgagtggct ggtgcgcgag gagctcaagg ggctgctggc cggctacccc
      601 gaaatccgtg tggagggaga ggctggctcc gtccggcagg cggcggagca gctcgcgcgg
      661 cgcgccttcg acgtcatctt tctggatatc cagatgcccg gcgccctcgg ctttgaactc
      721 ctcgagcagg ccgaggtcac tgccaggatc atcttcatca ccgcctacga tcagtacgcc
      781 atcaaggcct ttgaggtcaa cgccctcgat tatctgctca agcccatcca gaaggagcgc
      841 ctcggccagt cgattgcgcg cctgagcggc cgggcgcccg aagcgctgcg ccacaagccc
      901 aaactcggct acgacgatgt ggcttacgtc atggtcgccg gctcattaaa gttcctgcag
      961 gtggcgcaga tccgctgcat caccgccgga ggcaactatt cctatctcta ttatcaggac
     1021 caccggcggg agctggtggc caagagtctg caggagtggg aagagatcct gccgggggag
     1081 tatttccagc gcattcaccg ctcggcgctg gtcaactttg cctttgtcga gcgggtggaa
     1141 aagtgccgca attattcgca tctggtccac gtccgcgggg tagaaaagcc cctggtgatg
     1201 agccggcgtt actccgcccg gctgcaccac ctcctcccgc tgtgactcct tccgcttcta
     1261 tcccttgctg cccatctcct cgaggctttt acgctttaat ccgcggctgg taatgcggat
     1321 gcgggcgtgc accatgccct gctcttcggt cagggaaaag ttctgctcct ccctgaacat
     1381 gatttcgagg cgctgacgga tgttttccac agagttgccg tggccgtcgc tttgggtttt
     1441 ggccccgtcc ggcagaggct tgaggcggcc gctgttggag acatcgatgg cgagcacctc
     1501 atcccggtag cggatgttga gggtcacctg caggggcaga gggctggtct gcatgccgta
     1561 cttgatggcg ttttcgacca ggggctggca gatgaaggcg gggacgacca gctggcgcgc
     1621 cttctcgtcg atactgaccg cgacctggag ggaatcgcgg tagcgcacct tttcgatgtt
     1681 gaggtaattg gtgacggcgg tgatctcgtc gtcgagcagg gcctcggcct tgttaaaatt
     1741 gagcagcgag tagcgcagga attcggagag ggccgagacc atgtcccagg cctgttcctg
     1801 gctgatgagg atcatcgagc ggatcgagcc gagggcgttg aagaggaagt gggggttcag
     1861 ctggtagcgc agcagcttga gctgggagag gctcgccagg gagcgggcct tgagcaggtc
     1921 gcggcgctga cggatatagt cgatgaagag cttgaccagc atgagcaggg cggccagcgc
     1981 cagcagggtg aaccagtagg tcttccagaa ggggcgggag atggtgatgt ggatggcggt
     2041 cccctcttca ttccagatcc cgtcgttgtt ggcggccttg acccggaagg tgtaagcgcc
     2101 ggggtcgagg ttggtatagg ttacgaagcg gcggttggtg acgtggttcc attcgctctc
     2161 aaagccctcc atgatgtagg cgtactggtt ctgttcgctg agggcatagt tgagggcggc
     2221 aaattcgaaa gaaaagacgg tgtggcgcca ggagaggtcg atcctgcggg tgcggctgat
     2281 ctcctgtttg aggaggcggt tgggggcgtt gacctcgacg gggcggttga agagggcgaa
     2341 ttgcgtgatc accaccgggg ggatgaaggt gttttcgcgg atggcgccgg gataaaagcc
     2401 ggtggcgccg ttgatgcccc cgaagaagag ttctccgtcg cgggtcttga gggcggcacc
     2461 gtagttgaac tggttgctct gcaggccgtt ctccttgtag aagcacttga tgcggcgggt
     2521 ttcaggatcg aagcggcaga ggccgtcgct ggtgctcaac cacaggcagc cgctctcatc
     2581 ctcgaggagg ccgtggacga cattggcggg caggccgtcg cgggtggaaa aagcggtgac
     2641 ggcttcactg tcgggatcga ggcggaagag gccggctccg acggtgccga tccagatcat
     2701 ccccttgcgg tcctggagca gggcgcgcac ctgaatatcc cggccgctat cgttgagccc
     2761 gatgatgcga aagtccccgc tggcgggatc gaggcggccg acgctggtgc ggctgccgcc
     2821 gacccaaatg tgcccggcgc ggtcctgcag gagggagaga acgctgctga aaccctgatc
     2881 gtgccaggtg gtgaggaggt ggtcgaagcg gccggtgcgc ggattgaagc ggtcgaggcc
     2941 cccgccgtag gtgccgaccc agatctcgcc cgcttcgtcc tggaggaggg cgaggacgtt
     3001 gtcgttggcg aggctggccg gatccccggt ccggtgggtg tagcgggtga agcggcggcc
     3061 tgcggtgccg gggaggaggt tgaggccgcc gttgaaggtg ccgatccaga ggtttccccg
     3121 gcggtcctgg agcagggcct tgatgttgtt gacgctgagg caggagggat tgccgggctc
     3181 gtaggtatag taggagaaga ggccgctgcg gcggtcgagg cggttgatcc cgccgctttc
     3241 ggtgccgaac cagaggtcgc cgctgcggtc ctccatgatc gcataaacct cctgggagtt
     3301 gaggcagcgg cggctggcgg cgtcggcgcg gtagtggacg aaggggatgt tggtggtatt
     3361 gagaaaactg atgccgccgc gggtgccgat ccagaggtcg cccttgcggt cctggaagat
     3421 tgcctgcacc gagttctgca ccaggctgtg ggggttgttg gggtcatggg tgtggctggt
     3481 gatcaccccg ctgctgcgtt cgaggcggta gaggccgctg cggttgccaa tccagagtga
     3541 accgcggccg tcgtcgagga gggcgcggat ggtcgtgctt tcgggattgc ccgcgcccgg
     3601 gtggatagcg ctgaagcgcc cggtcgagcg ctccaggcgg ttgaggccgc cccaggcggt
     3661 gccgatccag aggtcgccct cgcggtccgc gcagatggaa tggatctcgt catcgctgag
     3721 ggaatgggga tcgccgcgga ggtggcgata gggggtaaag ctttctccgg agggatcgaa
     3781 ggagagcagg ccgccggagt tggtgccgac ccagagcagg ccgccgcggt cgagatggag
     3841 ggcgagtatt tcattggcgc cggcagcggc tttgccgggg taggggaaaa agctggcgat
     3901 cctgctgcgg tcgcggctga tgcggtcgag gccgttctcg gtgcccaccc agaggcagcc
     3961 gctgctgtcc aggagcatgg cgttgatatt gtcgccgctg agcagcagcc cggcactcga
     4021 gtccgcgccg aagcgctgaa agacgccgct gtcccggtcg aagcggttga ggccgccgtc
     4081 ttcagtgccg atccagaggt agccctgctc gtcctcggcc aggcagcgga tgaggttggc
     4141 ggagaggccg ccgggattgg cgggatcctg ctggtagacg acaaagtcgt agccgtcgta
     4201 gcggttgagc ccctcgttgg tgccgcacca gatgaagccc tggcggtcct ggagaaagca
     4261 gttcaccttg ctgtgggcaa gtccctgatc gatggtgagg ttgcggaact gcagctcgcg
     4321 ctcctggctg ttgctgggct gggcctcggc cccctgcacg ccggcggggg agagggcgag
     4381 gaagaagatg agaatgagaa tgaggcttgg cggtttcaag tcggaccttc ctcgattggg
     4441 ctgcgcgggg cagcgtggca ggggttggtc gccatggcag gcgggtcggc tgctcaattt
     4501 tacgtttttg ccgcctttta agcaaggaaa aactgcagct gtttgaccgt tcacataatc
     4561 tattaaccgt tcgccctttt ggggttgctt cgctggctat tttcatgtat actctggtat
     4621 aaccctgcat tcctgtccgc cgtccggccg atcatcccag ccgggcgcac catgcctgtt
     4681 gacctgcaga gtggcttgtg catgaaagag cttcggaccc tgatcatcga cgatgaatgg
     4741 ctcatccgcc tggaactgcg gcggatgctg cagtcgatgc ccaacatccg catcgtcggc
     4801 gaggccgcca cggtggccga ggcggcgcag ctcgccgaaa gcgccagtcc cgatctggtc
     4861 ttcctcgacc ttcagctcgg ggatggatcg ggagccgatt tcctcaacct tgcgcagggc
     4921 accttctcgc tcatcttcgt caccgccttt gaatgcaacc tccccaaggt caccaaccat
     4981 ccgaccctgg cctgtctggc caagcccatc aataaagaca agctttatgc tgtcctgcag
     5041 accctccaaa aaggtgaagg agtccaagta tgagaaagac cattctcttt gtgatcctga
     5101 ttctgctcac ctcattttcg gccctgctcg cccaggcgac gatcacgggc accgtcgccg
     5161 acagcgccaa gggggatccc ctcattggcg ccaacgtcta tctggtgggc actgctctcg
     5221 gcagcgtcac caatatcgag ggcgcctacc gcatcgtcgg cataccggag ggctcctaca
     5281 ccctgcgcat ctcctacatc ggctaccgga cccggaatct gcgcgtgcag cccaaggccg
     5341 gtgaaagcct cgaacgcaat gtgcggctgg tctccgacat cgccaagggc atcagcatgg
     5401 acgtcaccgc ccaggcagtg gggcagatgg cggccatcaa ccagcagatc agcgccaaga
     5461 ccatcgtcaa tgtgatttct gaagagaaga ttcaggagct tccggacgcc aacgccgccg
     5521 aatcgatcgg gcggctgccg ggggtctccc tgctgcgctc cggcggcgag gccaacaagg
     5581 tgatcctgcg cggtctggaa gacaagttca cggtcatcac tgtggacggg atcaagattc
     5641 cgcccaccga tgccaccagc cgcggcgtcg atctcagcac cctgtcgcag agctcgctgg
     5701 ccggcatcga actctacaag gcccagacct ccgacaagga tggcgatgcc ctcgccggca
     5761 gcatcaacct ggtcacccgc aaggccccgg cggagcgcga gatcaaggcc accctcaagg
     5821 gcggctacaa ccagctcgag gagaaggcca atcagtatga tgcctctttc cactatggcg
     5881 agaggttctt cggaagtctg ctcggcgtcc agctcgcggg caacatcgaa cagcgcatcc
     5941 gcagcaacga gcgcatcgat gtcgattaca accagagcct gaatttcggc cgcgactatt
     6001 ttatcaacga ctttactctt gaatacaccg atgagctgcg caagcgtgac ggcgccagcc
     6061 tcctgctcga cttcaacacc cctgacaacg gctcgatcaa gcttaacaac gtctacggcc
     6121 gcacccgccg cgactatttc ctctccaccc gcgactatcc ctccaacggc ggaggtgacc
     6181 agcagggcgc tcccaccttc aattttcgcg accgtgagca ggagatcaag accttgacca
     6241 gcgccctcag cggcgacaac cacctgctcg gccttaatct cagatggggc ctttcctacg
     6301 cccagtcgga agccgatttt cccttcgatt tccagaccat cttcgtcggc ctcgacggca
     6361 tgaaaccctc gccgatgctg cgcgagaatc ccgagcagct catcggctat gccaacaaga
     6421 actataacga agccggcctg agctgggcct acaaccgcgg ccagcacaac ctcgacaagg
     6481 agcgcaccgc ctttctggat gtcgcccgcc cctacaccct cagccggctc ttcagcggcg
     6541 aattcaagat cggcggcaag tacaaggtca aggatcgcag caacgaacgt tttgaggatt
     6601 tcacccccta ctacctcggc aaatggcagc cctacgaaat gctgccggat gggaccttcc
     6661 gcaacaagaa tttcgccggc tccgcctttg cggattgggc ctcgaccgcg atcagcggca
     6721 gcggcatcgc catcgaccgc tttttcaaag atccggtcac ccgcagcatc tacggctcct
     6781 acgatctcac ccccatcatc gaccgcgacc gcgtgcgcga gtggtacgag cttaaccgct
     6841 acggggtgga tgtgaccaag aacatccatg aggtctggaa caatcccctc tacgaatacg
     6901 acgattacga catcaccgag cgggtgggag cgggctacgt catgaacacc ctcaaccttg
     6961 gccgtttcgc caccgtgatc gccggcgtcc gcgtcgagaa ggaggagaat gactatctcg
     7021 ccggctacat gtccagaccc tcctcgggat ttccgatctc gcccaatgcg gtccgcgaca
     7081 ccacctcgga tgcctcgcag acggtggtgc tgcccaacgt caacgtcgcc ctcgccctct
     7141 ttgatttcat gaagctgcgc ctggcggcct tcaaggccct ggcccggccc gatttcaaca
     7201 tgcgcctcga ccgctatatc gccggccgcg gcgctgaggt gggcagcaat tttgaactcc
     7261 tggtcggcaa tcccgatctc aagaccgctg aagcctggaa ctttgaagtc aacccgacct
     7321 tcttcagcgg caccatcggt ctgatctctt tgtcggccta ttacaaagag atcgacgaca
     7381 tgtaccacat gctcaacgat ttcggcacca ccggcgacag cctgctccag cgtttcggca
     7441 tcggctggcc cagccagatg ctcggcaccg cctacagcct caccctgccc tacaactcgc
     7501 ccaagccgac caaggtgtgg ggattcgagt tcgagcacca ggtcaatttc aattttctgc
     7561 ccgggctgct gaagaatatc gtcctctcct acaacgcttc catcgtgcgc tcggaggcct
     7621 acatctggac cgccaggctg gattcggtct tttacgatcc ccccggtccg atcaagggag
     7681 cgtggaaaaa gttcaatacg ctccacgagg cgaaacggaa gctggagaac atgcccgagt
     7741 tctttggcaa catcgccctg ggctacgaca tcggccgctt ctccggcagg gtttcactgt
     7801 atcaccaggg tgagcacaac gtctcctact ccgccagcgg tctgagcgat gtcgtcacca
     7861 atgcctacac ccgcgtcgat ctcgccctca ggcagggcat cagtaaaaat atctcccttt
     7921 tcctcaatgt taacaatctt acggacgtcg aggagggcac ctcggtccgc aaccgggttt
     7981 tcgatcgcaa tctgttcaac atgagtgaaa aatacggcac gactgcagat ttcggtgtca
     8041 tcgcagagtt ctgagagcac aaaaccggac aggaggtgat gtaagccaga agttgagagc
     8101 agtacaaatc aaccgttttt caacaatcaa aggaggaaaa catgcgcttt cgagtacttg
     8161 cagtcctggc aatcgcgttg ctctgcagcg ccgcagtggt tttcgcccag gagacggtgg
     8221 ttccgatcac cccttctgat ggcagtgcgg cccttatgat caatgtgcag attgccgcgg
     8281 atacggtcgg ccgcggtttc cgcgcggacc gcgtctatga attcaagcgg ggagagatct
     8341 atctccagaa cgccaccctc actgtcccgg cgggccagac catccgctat cgggcgagtg
     8401 aaggtgacgg tcccctgccg gtcatctatc tctggccgac gggaacgggc tccaatccga
     8461 ccagaccccc gggctgggct atcaccctcg ccggcggcca tgcccgggtc agtgacctct
     8521 gcatggtggg tgtctatgag tatgagccta cctccttcga caacatgcag ggcggcctca
     8581 tccagaccaa cgccatcggc ggcagcatca tcctggataa tgtcatcctc tccaatacca
     8641 acggcaatca cgtccgcacc aacaatgccg ccaccaaggt gatcgccacc aactgtacct
     8701 tcgccaacat gggcaacctg gtgacctcga acttcggcgc cggcaaggcc ttcgacctgc
     8761 gcgacgttca ggtcgacacc ttcatcgtcg aaaactgcac ctttgtcaac ggccaggatc
     8821 gccccatccg ccactggaat gcgacctcga cccagccgat catctacggc cgcatcaacc
     8881 acaacacctt cgtcaacggc atgggcttcc acggactcct ctccctcggc ggtgtcggtg
     8941 aagagatgat catcaccaac aacctcttca ttgacgcctt cgccctcggc gaggactcaa
     9001 cggacgccac ccgcggtgcg gagtgggcca acactggtga agtgtacgct aacggtctca
     9061 acaagatcac ctggatcttt acgaccccga acgacaccac caagtggaag gtcagcaaca
     9121 atttcttcgc catcagcgat tccggccagg ccttcctgac caagttcggt ttcggtccgg
     9181 gcaatcccct ctccgctcat atccagagca agctgggcgc cggcgccgcc accgccttta
     9241 ccaagatcga cctcaccccg gtcaacatcc ccaagctgat gaccaacctc atgcgctggt
     9301 atgaggatcc cgcgggtggc aacaagacca aggacaaagc caactatgtc cgcgcccgcg
     9361 acgatttcga ccgccgcttc gtcgagtact tccgcgatac cctggatgtc tcctatgcca
     9421 ccaacctggc ggcctatacc ggcggcgagg cgggcttccc ggtcggcgac ctcaactggt
     9481 tcccggacaa gaaggcccag tgggagaacc aggcggcgat tcccaccatc gccagcatca
     9541 aggtcgatgc caatgctgat tttgtcccgg atctgaagga ccaggtcgta acgatccagg
     9601 gcgtcatcac caccattaac tattcgaaca agctgcagta ctatatgcag gatgctaccg
     9661 gcggcattaa cctctacagc ggcgcgatcg aaatggccct gaatatgggc gatgtcgtca
     9721 ccgtcaccgg ccagctccag cactataatg gtctgaccga gatcgtcccc gcggctgccg
     9781 ccgatgtggt tgtggtcggc acccaggctg tgcctgagcc cctgcccact acggccgccg
     9841 gtctcaacga ggagactgaa ggcatgctgg tcaaggtctt ccgctatcgc atcgtcg
//
LOCUS       DAOPWU010000103         9891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6891_length_9891_cov_4.949370, whole genome shotgun sequence.
ACCESSION   DAOPWU010000103 DAOPWU010000000
VERSION     DAOPWU010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6891_length_9891_cov_4.949370"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKM23_06970"
     CDS             complement(<1..285)
                     /locus_tag="PKM23_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY91253.1"
                     /translation="MKISTPYRHRSALLLPLLVLLLAAACARQGAPPGGPVDRKPPRI
                     MATAPRPDTTRVSLDSRIFLHFNESVEHRSCEESIFITPQPAEKPRYKWHG"
     gene            516..1574
                     /locus_tag="PKM23_06975"
     CDS             516..1574
                     /locus_tag="PKM23_06975"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNY91254.1"
                     /translation="MKKSPSDVTRREFLGGIAATGALAAWMTPLKSQAAPLPLVKPAP
                     LAEGDCVGLVTPASPVFEPSTVREGELAMQKLGYRVKIGRHIGRKWGYLAGSDAERAE
                     DLMQMFLDPEVKAIFALRGGYGTIRMLPLLDYGLIRSHPKVLIGYSDITSLHLAVHKL
                     AGMVTYHGAVATSTFNDYSTRYLKATLGRPAPVGMIETPEKALPMMIWPDRAPEVVHG
                     PLVGGNLTLVCASLGTPYEIDTAGKVLFLEETGEEPYDLDRMLTQLGQSGKLQAAAAI
                     LVDRCVNCKPAEYKPAFNNTMSTEEVIQDRLREMGVPVLYGLSIGHVADKPVLPLGVR
                     VAVHKKSASFSLEEAAVA"
     gene            1740..2753
                     /locus_tag="PKM23_06980"
     CDS             1740..2753
                     /locus_tag="PKM23_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91255.1"
                     /translation="MKERINTGPGYAGLLFFVVLTFWMGGARSGSAQSALLSWHAVTE
                     DTSGHAEVQPVYYNIYCDTVPAFRPDSANFLAATLQTSWTHTDPRLADPNRHLFYQVR
                     AVDIWGNQSAFSDTIGEVGYLLVKVRAFLQAPYDAEGDSMRTPLARLGLLPLASPYAA
                     APRAAAAAPGGAVDWVLLQLRDPTTANIVGEESFLLDREGFLTEPDGRNRVLGFTGCP
                     PGDYQLVLNHRNHVAVMSRGTFPFNEAPPVLHDFSADSTFYEGSGAACELEPGVWGLW
                     SGDINQDHQVSGLDFAYWQSAARDGRRGYLAEDLNFDGHITTADYVLWHRAHRRGVNS
                     YVP"
     gene            2829..>3646
                     /locus_tag="PKM23_06985"
     CDS             2829..>3646
                     /locus_tag="PKM23_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91256.1"
                     /translation="MKRVQLLLGLSLLLLGLSASAVLADASEFRLVVSSNAGTTGGRF
                     AVDLEIRGSASRTLNSLTADVFFGSELAAVAPPGENWFTGSGDYETSVTSISLPGTYY
                     RVLVTGNNIGKTSAGVPAGFTVTTSWQRVVTLVWSISTLRNSYNVNLLTYTDCAAYFD
                     NAANNPLADLTEWDSGTVPFATVKLAARAFLQGPYNTANHNMNTDLHTAALLPSASVY
                     TYDPRTVAVVPASVDWVMLQLRSSVTGPPVFSKSLFLRSDGQVVDEDGTENFDIP"
     assembly_gap    3647..3746
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3900..5003
                     /locus_tag="PKM23_06990"
     CDS             3900..5003
                     /locus_tag="PKM23_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY91257.1"
                     /translation="MKRILYATLIMLLALSANLMAAYNLQFEVVQNDMTNGGIFDVKV
                     QISSSGETFKMGTSNLVFTYNAAAVSPLPLTITPNASFSGGSYAAMTLTEPSSGRLSV
                     NIVLSSLNAGTLVGSAWMDVATIRFTIVDKTVNAALAWRITTPSACIVYKDDQITPVA
                     SGTLKPLDATLPVELSAFSAANINGVVRLSWTTQSEMNSLGFYINRREGNSGSFQRIS
                     GLIEGAGTSAAPRHYTFDDERLEIGRTYFYMLEQKDTNGQVSWHGPISVTVESVQLPE
                     AFYVEQNYPNPFNPTTTIEYGLPEAAQVRIRIYNMRGELVRTLVDGMQQAGNLVRVWD
                     GLDNNGSLVPSGVYFCRVQSGYEHKMIKMVFAK"
     gene            5161..6345
                     /locus_tag="PKM23_06995"
     CDS             5161..6345
                     /locus_tag="PKM23_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNY91258.1"
                     /translation="MDLSAIQHALQDAGLDGWLFYDFHNRDAIAARILGMDTHRFASR
                     RWYYYIPAHGEPQKLVHRIEPWRCDHLPGAKHIYLPWQEQHALLKTMLGGAQKVAMQY
                     SPNNAIPYVSIVDGGTVELLRSFGVEVISSADLVGRFEAHLSMEDWASHKEAMAVLHK
                     TKDAAFREVGRLIRAGVPPREIEIAEFMRSIMREHHLHFEDGPIVGINEHAGDPHFEP
                     TEENSLTMKEGDILLLDLWGKMDRPGSIYADITWMGYIGKSVPAALEEIFQVIRRGRD
                     AAVALVRERFAAGVTVYGWEVDDACRKVIVDAGYGAFFTHRTGHNIGEEVHGNGVHID
                     NLETKDERTIIPGSCFSVEPGIYMPEKKLGFRTEIDVVVTDEGEVTVEGGLQERIVPI
                     LA"
     gene            6547..7467
                     /locus_tag="PKM23_07000"
     CDS             6547..7467
                     /locus_tag="PKM23_07000"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HNY91259.1"
                     /translation="MSACQAQSGEKNMKYNKLTAEEERVILHKGTERPFTGKFTDHEE
                     KGTYLCKQCNAPLYRSEDKFHSGCGWPSFDDEIPGAVKRIPDADGSRTEIVCASCGGH
                     LGHVFTGEGFTDKNTRHCVNSISMTFVPAGEALPTPAFGAKPAVKQAKAWFAGGCFWG
                     VEHYFEKAAGVAAAVSGYMGGHVKNPTYQQVCTGTTGHYETVQVSYDPSRTNYEALAR
                     LFFEIHDPTQADGQGPDIGEQYRSVVFYSTPEEKATAEKLIGILKEKGYKVVTEVKPA
                     ATFWPAEGYHQDYYAHKGKTPYCHFYTKRF"
     gene            complement(7561..9285)
                     /locus_tag="PKM23_07005"
     CDS             complement(7561..9285)
                     /locus_tag="PKM23_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNY91260.1"
                     /translation="MKKWLICILLGLTQILLAQDPLQGMMEKNIRDRMMERTGSAPAL
                     TTATALENEIDPAEYYVGPGDQFLIRIEGTGNDNIEAAVSPEGELIIPAVGAIPVANH
                     PLSEAKSIIQEHLSAKYISRRIGIHLVKPRTFKVSVTGAVENPGYVEVRAMSRAAEAI
                     ELAGGLKQLLKVETVTQQVAIKSELREETSLQRTKPNPELRYNSSAGSKRNIRITRRS
                     GESVAVDLQKFALTGDRRANPYLRDGDVLFVPTEETSAGRLYIAGALKNPDIFEFAPG
                     DCIGDLIAMAHGFTTDADSSKIELVRFQGKGSSITKKVILLPADNPEARAEAMRFPLQ
                     PDDRLFVRFQYKFHETRNVEIEGEVLYPGFYALENGTVHLSEMIARAGGFTREASLKN
                     AYIQRRAQEDVLDPEYERLKKMAVLEMTESERDYFKIKARERVGGMGVDFVALFEQGD
                     KSQDVALRDHDLIHVPAQEQTVKVTGQVLNPGLYPYKPNMTVKHYLAEAGGYNWNARK
                     SRVRIIRSRTGEWAKPDNDSIIEVGDTIFIPEKPERDYWRLSRDLIAVAAQVATIFLV
                     VYNTTSGQ"
     gene            complement(9300..>9891)
                     /locus_tag="PKM23_07010"
     CDS             complement(9300..>9891)
                     /locus_tag="PKM23_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNY91261.1"
                     /translation="HFVGNVMIDSLREHLDKARACPVMEELGLSPRQYALVTLHRPSN
                     VDNPLLLDTILTAIGKLQEELTIVFPVHPRTEKMIAQFGFTPRLAAMKNLIRIEPLGY
                     LSFLRLMSEAAYLLTDSGGIQEETTWLDVPCLTLRESTERPVTVTLGSNRLVPLDTGA
                     IIEHAAMARSGLLKRKRIPPLWDGRASERIVNILLS"
BASE COUNT         1897 a         2909 c         3001 g         1984 t
ORIGIN      
        1 gccgtgccat ttatagcggg gcttttccgc cggctgcggg gtgatgaaga tcgactcttc
       61 gcagctgcgg tgttcgacgc tctcgttgaa gtggaggaaa atcctgctgt cgagggagac
      121 ccgggtggta tcgggccgcg gcgccgtggc catgatgcgc gggggctttc tgtcgacggg
      181 gccgcccggc ggcgcaccct ggcgggcgca ggcggccgcc agcagcagga ccaggagcgg
      241 cagcagaagc gcggagcgat gacggtatgg agtggatatc ttcatggttc agggccctgc
      301 aaagagtggg tcggatacct aaaataaaaa ttaaccactg caatatcaag gcctttttcg
      361 catccgggta actccgaaat ctggttccca cctgccacct tgcgctgcgt actctgatag
      421 ctccaccagg caaaaatctg ttttgaaatg agcgcatttt tatttagaat gaacaaaaca
      481 gcctgaagaa cggtgttgag aacgaggtgc aagtgatgaa aaagagtcct tctgatgtga
      541 cgcggcggga gttcctgggc ggtatagccg ccaccggtgc cctggcggcc tggatgacgc
      601 cgctgaagag tcaggccgct cctcttcctt tggtcaagcc agcccccctg gcggaaggag
      661 attgcgtggg gctggtcact cccgccagcc ccgtcttcga gcccagcacg gtgcgcgagg
      721 gtgagctggc gatgcaaaaa ctgggctacc gcgtcaagat cgggcgccat atcggtcgca
      781 aatggggcta tctcgccgga tcggacgccg agcgcgccga ggatttgatg cagatgttcc
      841 tcgatccgga ggtcaaggcc atcttcgccc tgcgcggggg gtatggcacc atccgcatgc
      901 tccccctgct cgactatgga ctcatccgtt cccatcccaa ggtcctgatc ggctacagcg
      961 acatcaccag cctccatctg gctgtccaca agctggccgg catggtgacc taccacggcg
     1021 ccgtggccac ctccacgttc aacgactatt cgacgcgcta ccttaaagcc accctgggcc
     1081 ggccggcccc ggtggggatg atcgaaaccc ccgagaaggc gctgccgatg atgatctggc
     1141 ccgaccgtgc gccggaggtg gtgcacggcc ccctggttgg aggcaacctc accctggtct
     1201 gcgccagcct cggcactccc tacgagatcg acaccgcggg caaggtgctc tttctcgagg
     1261 agaccggtga ggagccctat gacctggacc ggatgctgac ccagctcggg cagagcggca
     1321 agctccaggc ggccgcagcg attctggtcg accgctgcgt caactgcaag cccgccgagt
     1381 acaaacccgc ctttaataat accatgagca cggaggaggt catccaggac cgcctgcggg
     1441 agatgggtgt gccggtgctc tacgggcttt caatcggcca tgtcgccgac aagccggtcc
     1501 tgcccctcgg ggtgagggtg gcggtgcata aaaaaagcgc ctcgttttcg ctcgaagagg
     1561 cggccgtcgc gtaaaaattt ccgcactttg gcggtacttc cgtatatgaa aagtgtaggg
     1621 aatatctcgg caagtgtgag aagcgtcacc gcgatttgtc tgcagaaatc ataccttgtg
     1681 atggctgctc cctgtttatt gcgttggaaa taatgatcta aggtatgatt ttgaactgca
     1741 tgaaagagag aatcaatacc gggccgggct atgccggtct gttgttcttt gtcgtcttga
     1801 cgttctggat gggcggtgcc cggagcggat cggcgcagag cgcgctcctc tcctggcacg
     1861 cggtcaccga ggataccagc ggccatgcgg aggtgcagcc ggtctactac aacatctact
     1921 gcgataccgt gccggccttc cgtcccgatt cggccaattt tctcgccgcc actttacaga
     1981 ccagctggac tcacaccgat ccccggctgg ccgatcccaa ccggcacctc ttttaccagg
     2041 tgcgggcggt cgatatctgg ggcaaccaat cggcgttcag cgatacgatt ggggaggtgg
     2101 gctatctcct ggtaaaggtg cgcgcctttc tgcaggcccc ctatgatgcg gagggcgatt
     2161 ccatgcgcac tccactggct cggctggggc ttctgcccct ggcatccccc tatgctgcgg
     2221 ccccgcgggc tgccgcggct gcgcccgggg gggcggtgga ctgggtgctc ctgcagcttc
     2281 gtgatcccac cacggccaac atcgtcggtg aggagtcctt tctgctcgac cgggagggtt
     2341 ttctcaccga accggacggc cgaaaccggg tgctgggctt caccggttgc ccgcccggcg
     2401 actaccagct ggtcctgaat caccgtaatc atgtggcggt gatgtcgcgc ggcacctttc
     2461 ctttcaatga ggcgccgcct gtgctgcacg atttcagcgc cgacagcact ttttacgagg
     2521 gcagcggtgc cgcctgcgag ctggaaccag gggtgtgggg gctctggagc ggcgacatca
     2581 accaggacca tcaggtttcc ggccttgatt ttgcttactg gcagagcgcg gccagggacg
     2641 gtagaagagg ctatctggcc gaggatctca atttcgacgg ccatatcacc actgcggatt
     2701 acgtgctctg gcaccgggcc caccgccgcg gcgtcaacag ctacgtgccc tgatcaattt
     2761 ttgtttgaaa cggccaatta ttcctgtata ttgttactgg aaccctaaaa ggggaagtgg
     2821 aagaaatgat gaaaagagtg caacttttgc ttggcctgtc cctcctcctg ctcggattgt
     2881 cggcgtccgc agtgctggcg gatgcctccg aattccgtct cgtggtctcg agcaatgcgg
     2941 gcacaacggg cgggcgtttc gccgtggatc tggaaatcag gggatcggcc agccgcaccc
     3001 tgaattcgct caccgcggat gtgtttttcg gatcggaact cgcggcagtc gcgcctcccg
     3061 gtgaaaactg gtttacgggc agcggcgatt atgaaacctc ggtgacctcg atcagtcttc
     3121 ccggcaccta ctaccgcgtg ctggtgaccg gcaacaacat cggcaagacc agcgccggtg
     3181 tgcccgcggg ctttaccgtg acgaccagct ggcagcgcgt ggtcaccctg gtctggagca
     3241 tctccaccct gcgcaacagc tataacgtca acttgctgac ttatacggat tgcgccgcct
     3301 atttcgacaa tgccgccaac aatcctttgg cagacctgac ggaatgggac agcggcacag
     3361 tcccctttgc caccgtcaaa ctggcggcca gggctttcct gcaggggccc tataacactg
     3421 ccaatcacaa catgaatacc gatctccata cagctgcgct gcttccctcg gcctcggtct
     3481 atacctatga tccgcgcact gtcgccgtgg tccccgcttc ggtcgactgg gtcatgctgc
     3541 agctgcgcag ttcggtcacc ggtccgccgg tcttttcaaa aagcctcttc ctgcgcagtg
     3601 acggccaggt ggtggatgag gacgggacgg agaatttcga cataccnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnngcct ggttcctcaa caatggcacc gcctggacct
     3781 atgccaagtg gtccgatttc aatctcgatg gcggcatcga tgccatcgat gccaattcct
     3841 gctggcgccc caacaacggc agcgccaccc aggtacccta atcgcagatg aggatgaaaa
     3901 tgaaaagaat actctacgca accctgatca tgctgctggc cttgagcgcg aacctcatgg
     3961 ccgcctacaa tctgcaattc gaagtcgtcc agaatgacat gaccaatggc ggtatctttg
     4021 acgtcaaggt gcagatctcc agcagcggcg agaccttcaa gatggggacc tcgaacctgg
     4081 tcttcaccta taacgctgcg gccgtttccc ctctgccgct caccatcacg cccaacgcct
     4141 ccttcagcgg cggaagctat gcggccatga ctctgactga accctcctcc ggccggctct
     4201 cggtcaatat cgttctctcc tccctcaatg caggaaccct ggtcggctcc gcctggatgg
     4261 atgtggcgac gatccgcttc accattgtcg ataaaacggt gaacgcggcg ctggcctggc
     4321 ggataaccac cccgagtgcc tgtatcgtct acaaggatga tcagattacc ccggtcgcct
     4381 cgggcaccct caagccactc gacgccaccc tgccggtgga gctgagcgct ttttccgcgg
     4441 ccaatataaa cggtgtcgtc cggctctcct ggaccaccca gagtgaaatg aattctctcg
     4501 gcttttatat caaccgccgg gaagggaatt cgggcagttt ccagcgcatc agcgggctga
     4561 tcgagggagc gggcacttcc gcggctcccc ggcactatac cttcgatgac gagcgccttg
     4621 aaataggacg cacctatttc tatatgctcg aacagaagga taccaatggt caggtgagct
     4681 ggcacgggcc catcagcgtc accgtcgaaa gcgtgcagct gccggaggct ttttacgtcg
     4741 agcagaatta tcccaatccc ttcaatccca ccaccaccat cgagtacggc ctgcccgagg
     4801 cggcccaggt gcgcatccgg atctacaaca tgcgcggcga actggtccgc accctggtgg
     4861 acggcatgca gcaggccggc aatctggtcc gggtctggga cggcctcgac aacaatggct
     4921 cgctggtccc aagcggggtc tatttctgcc gggtgcagag cggctacgaa cacaaaatga
     4981 tcaagatggt cttcgccaag taagcgggac caaagctctt cagggctgtc cggtgcaagc
     5041 gggcagccct tttttattgg acactctcct cctcaccgaa gaaatcccgg ccataaaaga
     5101 gttgcatttc ccgcggatat tttttacttt cctgagacaa actcaaaaag agaggtgagc
     5161 atggatctat ccgccattca gcacgccctg caggacgccg gcctcgatgg ctggctcttt
     5221 tatgattttc acaaccgtga cgccatcgcc gcgcgcattc tcggcatgga cacccaccgt
     5281 tttgcctcgc ggcgctggta ctattacatc ccggcccacg gcgagccgca gaagctggtg
     5341 caccgcatcg aaccctggcg ctgcgatcac ctccccggcg ccaaacacat ctatctgccc
     5401 tggcaggaac agcacgccct gctcaaaacc atgctgggcg gcgcccaaaa agtggcgatg
     5461 cagtattcgc ccaacaacgc catcccctac gtctccatcg tcgacggcgg cacggtcgag
     5521 ctgctgcgca gctttggggt tgaggtgatc tccagcgccg atctggtcgg ccgcttcgag
     5581 gcccatctct cgatggagga ctgggccagc cacaaggagg cgatggcggt gctgcacaag
     5641 accaaggatg ccgcctttcg ggaagtgggc cggctgatcc gcgccggtgt gcccccgcgc
     5701 gagatcgaga tcgccgaatt catgcgcagc atcatgcgcg aacaccatct ccatttcgag
     5761 gacggcccga ttgtcggcat caacgagcat gctggcgacc cccatttcga gccgacagaa
     5821 gagaacagtt taaccatgaa agagggggat atcctgctgc tcgatctctg gggcaagatg
     5881 gaccggcccg gatcgattta tgccgacatc acctggatgg gctacatcgg caagagcgtg
     5941 cccgccgcgc tcgaggagat ctttcaggtc atccgccgcg gccgcgacgc cgcggtcgcc
     6001 ctggtgcgcg agcgcttcgc cgccggcgtg accgtctacg gctgggaggt cgatgacgcc
     6061 tgccgcaagg tgatcgtgga tgcgggatac ggcgcgtttt tcacccaccg taccggccac
     6121 aatatcggcg aggaggtgca cggcaacggg gtgcacatcg acaacctcga gaccaaggat
     6181 gaacgcacca tcatcccggg cagctgtttc tcggtcgagc cggggattta tatgccggag
     6241 aagaagcttg gcttccgcac cgaaatcgac gtcgtcgtca ccgacgaggg cgaggtgact
     6301 gttgaaggcg gcctgcagga gaggatcgtg ccgatcctgg cctgacctcc gcactagttc
     6361 tgctaccaat tcaggcgtgc gcgctggttg ctgcgcacgc cttttttctt ttgtgccgct
     6421 gcgatccggc gggaatagtc ggcagggatg ctagcgttta tagtgtacaa ttttcgtgat
     6481 gagcgggaga aatactgtat gaaaataaaa agaatttcca tgctgatatg ggcaggcgtg
     6541 ctcgcgatga gcgcctgtca ggcacaatcc ggagagaaga atatgaaata caacaaattg
     6601 acggcggaag aggagcgggt catcctgcac aaggggacgg agcggccctt caccggtaaa
     6661 tttactgacc acgaggaaaa aggcacctac ctctgcaaac agtgcaacgc acccctctat
     6721 cgctcggaag acaaatttca ttcgggctgc ggctggccca gcttcgacga tgaaatcccg
     6781 ggcgcggtca agcgcatccc tgatgccgac gggtcgcgga cggaaatcgt ctgtgccagc
     6841 tgcggcggcc atctcgggca tgtcttcacg ggcgaaggct ttacggacaa gaatacccgc
     6901 cactgcgtca actcgatctc gatgaccttc gtgccggcgg gtgaagcgct gcccaccccg
     6961 gcctttggtg ccaagcccgc ggtgaagcag gccaaagcct ggttcgcggg cggctgcttc
     7021 tggggggtcg agcactattt cgaaaaggcc gcaggggtgg ctgcagcggt ttcggggtat
     7081 atgggcgggc atgtcaagaa tcccacctac cagcaggtct gcaccggcac caccggccac
     7141 tacgagaccg tccaggtgag ctatgacccc tcccggacca actatgaagc cctggcgcgg
     7201 ctctttttcg agatccacga tcctacccag gccgatggcc agggcccgga tatcggcgag
     7261 cagtaccgtt cggtggtctt ctattcaacc ccggaggaga aagcgacggc ggaaaagctg
     7321 atcgggatcc tcaaggagaa gggctacaag gtggtcacgg aggtcaagcc ggcggcgact
     7381 ttctggccgg ccgagggcta ccatcaggac tattatgccc acaagggtaa aaccccctac
     7441 tgccattttt ataccaagcg gttctgatcc tcccttcacg gcgatcatcc ctgaacaaaa
     7501 aacggcggac aggagaattc ctgtccgccg tttcattttc caggcctgca gctgcgccgt
     7561 tcactgtccg gaagtggtgt tgtagaccac aagaaagatc gttgccactt gggcggcgac
     7621 agcgatgaga tcccgggaga gccgccagta atcgcgttcc ggtttttcgg ggatgaagat
     7681 ggtgtccccg acttcgatga tggaatcatt gtccggtttg gcccactctc cggtccggct
     7741 gcggatgatg cggacccggc tcttccgggc gttccagtta tacccccccg cttcggcgag
     7801 ataatgtttg acggtcatgt tgggcttgta gggatagagg ccggggttga gcacctgccc
     7861 ggtcaccttg acggtctgtt cctgggccgg gacatggatg aggtcatggt cgcggagggc
     7921 gacatcctgg gatttgtcgc cctgttcgaa gagggcgaca aaatcgacgc ccatgccgcc
     7981 gacgcgctcg cgcgctttga tcttgaaata atctcgctcg ctctcggtca tctccagcac
     8041 ggccatcttt ttcaggcgtt catactccgg atcgaggaca tcctcctggg cgcggcgctg
     8101 gatataggcg ttcttgagcg aggcctcgcg ggtaaagccg ccggcgcgtg cgatcatctc
     8161 gctgagatgg accgttccat tctccagtgc gtaaaaaccc gggtagagca cctctccctc
     8221 gatctcgaca ttgcgagtct catggaattt gtactgaaaa cggacgaaga ggcggtcatc
     8281 gggctgcagg ggaaagcgca tcgcctcagc gcgcgcctcg ggattgtcgg ccggcagcag
     8341 gataactttc ttggtgatgc tggagccctt gccctggaag cgcaccagct cgattttgct
     8401 cgaatccgcg tcggtggtga agccatgggc catggcaata aggtcgccga tgcagtcgcc
     8461 gggggcgaat tcaaagatat cgggattctt cagggcgccg gcgatgtaga gacgaccggc
     8521 gctggtctcc tcggtcggga cgaagaggac gtcaccatcg cgcagatagg gattggcgcg
     8581 ccggtcgccg gtgagggcga acttttgcag gtcgacggcc acgctctcac cgctgcggcg
     8641 ggtgatgcgg atattgcgct tcgagccggc agaagaatta tatctgagtt ccggattggg
     8701 cttggttcgc tgcagcgagg tctcttcgcg cagttcgctt ttgatggcga cctgctgggt
     8761 cacggtttcc accttgagca gctgcttcaa tccgccggcc agctcgatgg cctcggcggc
     8821 gcggctcatg gcgcggacct cgacatagcc gggattttcc actgcgccgg tgaccgagac
     8881 cttgaaggtg cgcggcttga ccagatggat gccgatccgc ctgctgatat atttggcgga
     8941 gagatgttcc tggatgatac tcttggcctc actcagggga tggttggcga cggggatggc
     9001 cccgacagcg gggatgatca gctcgccttc ggggctgaca gccgcttcga tgttgtcatt
     9061 gccggtgccc tcgatgcgga tgaggaactg gtcgccgggg ccgacatagt attcggccgg
     9121 atcgatctcg ttttcgagcg ctgtcgccgt ggtcagcgcc ggggccgacc cggtgcgctc
     9181 catcatgcgg tcgcggatat ttttctccat catgccctgc aggggatcct gggcgagaag
     9241 gatctgcgtc agcccgagga ggatgcaaat cagccatttt ttcatatcat ttccacctat
     9301 catgaaagca gaatgttgac gatgcgttcg ctggcccgtc cgtcccaaag cggggggatg
     9361 cgtttgcgct tgagcagtcc ggagcgggcc atcgcggcgt gttcaatgat ggcgccggta
     9421 tcgagcggga ccaggcggtt gctgcccagg gtcaccgtaa ccggccgttc cgtgctttcg
     9481 cgcagggtta ggcagggcac gtcgagccag gtggtctcct cctgaatgcc gcccgagtcg
     9541 gtgagcagat aggccgcttc gctcatcaag cgcaggaagg agaggtaacc gaggggttcg
     9601 atgcggatga ggtttttcat cgccgccagg cgcggagtga agccgaattg ggcgatcatc
     9661 ttttcggtgc gcgggtggac cggaaagacg atggtcagct cctcctgcag cttgccgatg
     9721 gcggtgagga tggtgtcgag gaggaggggg ttgtcgacgt tggaggggcg atgcagagtc
     9781 accagggcgt attgtcgggg agagagtccg agctcctcca tgaccgggca agcgcgggcc
     9841 ttgtcgaggt gttcacgcag ggagtcgatc atgacattgc cgacaaagtg c
//
LOCUS       DAOPWU010000104         9887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6896_length_9887_cov_4.901749, whole genome shotgun sequence.
ACCESSION   DAOPWU010000104 DAOPWU010000000
VERSION     DAOPWU010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6896_length_9887_cov_4.901749"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PKM23_07015"
     CDS             <1..960
                     /locus_tag="PKM23_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HNY91262.1"
                     /translation="LPPPKTSSPQRTCIGCRSCKPADELMHLAYDSEQAVRFDPRREL
                     PGRGCHLCPDQRCLESALRSRAFARAYRRAVPVSALAGLKDEFAAARAAWIAAFRPGR
                     RTALITERLESAFGEPVWQSGEDPLAALILTVLSQSTNDRNRDFAFQRLRTAFPDWES
                     VSRAPSTAIAEAIRPAGLANQKSVRIRDILHWVKATYGGFDLSPLCGQDPREVSATFM
                     QLKGIGIKTISVVLMVACGANVFPVDTHVHRICQRLGLVPQGLSAEKTHELMQPLVPP
                     GKSYSLHMNFLRLGRTICQARKPRCAECPVALWCPSSSLFESE"
     gene            964..3999
                     /locus_tag="PKM23_07020"
     CDS             964..3999
                     /locus_tag="PKM23_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNY91263.1"
                     /translation="MKRLITLFLLAAVLSAFAAPIDVTFRHYPTAPTVQRAFVPGSFN
                     NWGPNASGVITTGAPSEMAWADSLGCYIKQYTLETGVTYNYKFHEHLNAAGSQWQWLR
                     DPLNPLINTADNNNSLLTPEPIMLFEIRPAEGALITDQGAWLTAGAFAAAGDSLLLSA
                     STIIIDGAVASTLEGCWLGGLGLIHYPLPALANGSHSLTIRLAARSGAVVEETVTFST
                     LTGDLFFMTPSCDSLLAAERIVRWKVNRPFEQIAAISLRQSGKAAVDIMPEPDGEYAR
                     SVACSRGVNTFVLSLTDRAGATIHSDTLFLRYPLPQHPVPGVALSRSGDKIKITGSAR
                     DPQNKPMSYRWSTSVLNPALLPGVNGSSELVLEIDPPTRPGDYSLELTATDADGLSSS
                     AESFFTILADGSVVLPGSATVPQWVREARIYCIFFKAFSAGGRIAEAIPRLGYIKAMG
                     FNTIWVLPVMDVEGNIDQGANIGYNIIDFFHVDPDYGTDEDFREFVRRAHEAGLRVIL
                     DVTPNHSSRSHPCALDARANRKYSRYYDFFQHEPINHDTNGLGQSISGDGIVYYSAFS
                     DALVNWNWSDAEARSYMLEVYKYWLREFDIDGFRFDVWWGPHRRYGVGAFDKPLRLAL
                     RTVKSNILLLGETAGTGSGTEDNYADQGGGMDMGYDWNLFGGLSAYPNPGALQIRLYN
                     SGFRPGPNSFFLRFLENHDEDRVIYRYGSGEKTIPVSTALFMATGTPLLYAGQEAGMG
                     LYGGTLDARRRGTINWSGLYAAILTPHYQKLAQIRAQFPAFRTQFEDSNSDGRIIGPD
                     EDVQRRLDQGSATSYVMARPWLDANGLVVMNFAGTAQEFTIALKAGEWMRFSAGFSAQ
                     GSYWLNNLYDNTSRPVSGAELDTLKVSLPPYGVAIYTIATAEQRLVLPDLEVGVEHKS
                     PAELPQAFHLAGSWPNPFNSATAIRFTLHQAERIEAEIFDALGRRVRSLTAAALPAGE
                     HELHWDGRNQTGMHCPSGLYFLRLRHGSQTAVHKMALVR"
     gene            4054..6159
                     /locus_tag="PKM23_07025"
     CDS             4054..6159
                     /locus_tag="PKM23_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HNY91264.1"
                     /translation="MSYHRLLRPWAATMLLILAALAGAQTWTADNGNGSFTNPLFYEE
                     FSDPDIIRVGDDYYLTGTTMHAMPGLPVLHSRDLVNWKLLGYAAERLNLGPELRFEEG
                     REAYGQGIWAPCFRYHAGTFYIFANVNGHGTQLYTANTPAGPWRHQTMRAALHDVSVL
                     FDDDGKVYAVWGYDELHMAQLTPDLLDIVPGTERVVVARGSGAGEGCHFYKIDGRYFI
                     TSTNYDPVCYQVCLRAEAPYGPYELRVISAEENLGVGTGWRLWNTREGPPFALQPPIE
                     NFVGCIPMHQGGIIRIQSGEWWGWSMLDHNSVGRVTALSPVTWVEGWPLFGLPGNLTR
                     TPRTWIKPNTGFSSPPSAPFERNDEFSGPGLKNGWQWNHHPVEEKWSLTERQGFLRLH
                     ALPAEDFWRARNSLTQRGIGPESIATAELDPAGLQAGDVAGLALLNLPWAWIGIARLE
                     EGMELQFCNQQALELLRVPVTAKKVWLRAHCNFDTDTGRFAYSLDGSRFTEIGGEIIM
                     PYQLKTFQGVRYALFCYTTRGEEGGWADFDRFTVTEPRCQGLTRPIPHGRLITLTSLA
                     DSTVLVNWRNHLRPAERTSPFAAGDASLFRVLDRGQGRIALQSTATGGYVTVKGEAGL
                     AEVRIEAAEQGEASLFQWVDMLRGDLMLLSLHSHRYLFADPEARSLCSADARSARPDR
                     RDGSCFSWQLAAENQQARE"
     assembly_gap    6264..6273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6274..7283
                     /locus_tag="PKM23_07030"
     CDS             <6274..7283
                     /locus_tag="PKM23_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNY91265.1"
                     /translation="GESLALPIAAFQSRAASAPRAPGVYLMKDGDGRILYVGKSIDIR
                     SRLRQHAASLRAGYGERNYRWIRQVRELSWLETNSELYALLLEDQLIKAHWPAGNVRQ
                     KEYLEYAWLAFSVEALPRLQVIDARQRGQFDAVFGPFHDLYHARDMADLVKMRFRLRT
                     CAAVRAEGCLQGEMRKCSAPCRTAEAAERYRGTVARAAASLQRHDPWFFRFIDYTILR
                     CSRNREFERAAWLHAMRHRYCALIRRQDFIEHFKRHGLVVREGGRWENTFYFLHGRMI
                     RREGGELLAGEAPAEEGVLSEWQIIDRAQVIWAWLQGGRISGAAAVIDARFFRAEIAD
                     R"
     gene            complement(7289..8308)
                     /locus_tag="PKM23_07035"
     CDS             complement(7289..8308)
                     /locus_tag="PKM23_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNY91266.1"
                     /translation="MIRILFLADTHLGIDTPLRPRIQRRRRGDDFYANYTAALQPAAA
                     GKVDLVVHGGDMFFRSRVHPSIIESAFAPLLQIADLGVPVCIVPGNHERSNIPCSLLE
                     SHPQIAIFDHPLTLVLTLRGLRIAVTGLPCIRHNPAGLFVPEVEKSGWRDCSADIRLL
                     CMHQAVEGAQVGVQNYTFRSGEEAIRGRDIPAGFDAVLSGHIHRHQVLTRDLAGRRLN
                     APVFYPGSIERTSFAEREEEKGYLIIELAPGGGIRHRFMPLPARPMIDLTVDGSAATL
                     EEVRAQLIRQIAALDAEAIVRLRPAASIPAALLSALSERWLRSVAPSTMNISWGIPRY
                     QAPAG"
     gene            complement(8305..8739)
                     /locus_tag="PKM23_07040"
     CDS             complement(8305..8739)
                     /locus_tag="PKM23_07040"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNY91267.1"
                     /translation="MRILILCTGNSCRSQMAEAFLKHFNPALEVESAGTEPAATVHPL
                     AVEVMAEKHIDISQARPKSVAIFTHLPFHYVITVCDNARESCPVFLGEVKQELHIGFD
                     DPAAVEGTEEEKLAAFRRVRDEIHEAFKKFYLEHVVQREQGN"
     gene            9155..>9887
                     /locus_tag="PKM23_07045"
     CDS             9155..>9887
                     /locus_tag="PKM23_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNY91268.1"
                     /translation="MEELFGKIGKSLTLSQEIELRIEDSIRQKKLEPGQKLPSEKELG
                     VIFGVSRTAVREALRMLSARGLLHVRKGDGVYVNSFSSSLASKSMSLYLELQFDTDSI
                     LHLVHLRQLIEPNLARLAAQKRSEEDLVRFRLNLAAFSDRTQDADQLARLDVEFHTLI
                     VTSSHNPLIPLIMDPIFSMLPRVKTLIVNHLQKTHSDNAPNYHQKIYDMIRLQDGEGA
                     FSAMQEHLKIAERDSLLLAQWMKENQ"
BASE COUNT         1853 a         3358 c         2885 g         1781 t
ORIGIN      
        1 ctgccgcccc cgaaaacctc atccccgcag cgcacatgca tcggctgccg cagctgcaag
       61 cctgccgatg agctcatgca cctggcctac gattccgagc aggccgtccg cttcgatccg
      121 cgccgtgagc tgccgggccg cggctgtcac ctctgccccg accagcgctg tctcgaaagc
      181 gcgctgcgga gccgggcttt cgcccgcgcc tatcgccgcg cagttcccgt atcagcgctt
      241 gcaggactga aagatgagtt tgccgccgct cgggcggcct ggatcgccgc cttcaggcca
      301 ggacgccgca ccgccctgat cacagagcgc ctcgaatccg ccttcggcga acccgtctgg
      361 caatccggtg aggatcccct cgccgccctc atcctcaccg tgctctcgca gagcaccaac
      421 gaccgcaacc gcgacttcgc cttccagcgt ctgcgcacag cctttcccga ctgggagtcc
      481 gtgagcagag ccccgagcac agccatcgcc gaagcgatcc gcccggccgg actggccaac
      541 cagaagagcg tgcgcatccg cgacatcctg cactgggtca aggccaccta cggcggcttc
      601 gacctcagcc ccctctgcgg gcaggacccc cgcgaggtca gcgcgacctt catgcagctc
      661 aaggggatcg gcatcaagac catcagcgtg gtgctgatgg tggcctgcgg cgccaatgtc
      721 ttccccgtcg atacccatgt ccaccgcatc tgccagcgcc tcggcctggt gccgcagggg
      781 ctgagcgctg aaaagaccca tgagctcatg cagcccctcg tcccgccggg caagagctac
      841 tccctgcata tgaattttct caggctgggc cggaccatct gccaggcgcg caagccccgc
      901 tgcgccgaat gcccggtcgc cctctggtgc ccctcatctt ccctatttga aagcgaataa
      961 cccatgaagc gcttgatcac cctcttcctg ctggcggcgg tcctctccgc ctttgccgcc
     1021 ccgattgatg tcaccttccg tcactacccg acggccccga ccgtgcagcg cgccttcgtc
     1081 cccggcagct ttaacaactg gggccctaac gccagcggcg tcatcaccac cggcgccccc
     1141 tccgagatgg cctgggccga cagcctcggc tgctacatca aacagtatac tctcgagacc
     1201 ggcgttacct acaattacaa gttccatgag cacctcaacg ccgccggatc gcagtggcag
     1261 tggctgcgcg atcccctcaa tccccttatc aacaccgccg acaacaacaa ctctttgctc
     1321 acccctgagc cgatcatgct cttcgagatc aggccggccg agggagccct gatcaccgac
     1381 cagggtgcct ggctcaccgc cggggccttc gccgccgccg gcgattccct gctcctttcc
     1441 gcctcgacca tcatcatcga cggcgcggtg gcctccactc tggaaggatg ctggctgggc
     1501 ggcctggggc tgatccatta cccactccct gccctggcca acggcagcca cagcctcacc
     1561 atccgcctgg ctgcccgcag cggggccgtc gtcgaggaga cggtcacctt ctcgaccctg
     1621 accggggatc ttttcttcat gacgccctca tgcgacagcc tcctggccgc ggagaggatc
     1681 gtgcgctgga aggtcaaccg ccccttcgag cagatcgccg ccatcagcct gcgtcagtcg
     1741 ggcaaagccg ccgtcgatat catgcccgag ccggacgggg aatacgcccg cagcgtcgcc
     1801 tgcagccgcg gggtcaacac cttcgtgctc agcttgaccg accgcgccgg cgcgaccatc
     1861 cacagtgaca ccctcttcct gcgctatccc ctgccgcagc acccggtgcc cggcgtcgcc
     1921 ctctcgcgca gcggggacaa gatcaaaatt accggcagcg cccgcgatcc gcagaacaaa
     1981 ccgatgagct atcgctggtc cacttcggtg ctcaatccgg ccctcctgcc gggagttaat
     2041 ggcagcagcg aactggtgct ggagatcgat ccccccacac ggcccgggga ttacagcctc
     2101 gagctcaccg ccaccgacgc cgacggcctc agcagcagcg ccgagtcctt cttcaccatc
     2161 ctcgccgacg gcagcgtcgt tctgcccggc tccgcgacgg tgccgcagtg ggtgcgcgag
     2221 gcccgcatct actgcatctt cttcaaggcc ttctcggcag gcggacgcat cgccgaagcc
     2281 atcccccgcc tgggctatat caaggcgatg ggcttcaaca ccatctgggt gctgccggtg
     2341 atggacgtcg agggtaatat tgaccagggc gccaatatcg gctacaacat tatcgatttt
     2401 ttccatgtcg atccggacta cggcacagat gaggatttcc gggagttcgt gcggcgcgcc
     2461 cacgaggccg ggctgcgggt gatcctcgac gtcaccccca accactccag ccgctcccat
     2521 ccctgcgccc tcgacgcccg cgccaatcgc aaatacagcc gttattacga tttttttcaa
     2581 cacgaaccga tcaaccacga caccaacggc ctggggcagt cgatctccgg cgatggcatc
     2641 gtctattaca gcgctttttc cgatgccctg gtcaactgga actggagcga cgccgaggcg
     2701 cgcagctaca tgctcgaggt ctacaagtac tggctgcgcg aattcgatat cgacggcttt
     2761 cgctttgatg tctggtgggg cccccaccgc cgttacggag ttggcgcctt cgacaagcct
     2821 ctgcgcctcg ccctgcgcac ggtcaagtcg aatatcctcc tgctcggcga gaccgcgggc
     2881 accggcagcg gcacggagga taactatgcc gaccagggcg gcggcatgga catgggctac
     2941 gactggaacc tcttcggcgg cctcagcgcc taccccaatc ccggcgccct gcagatccgc
     3001 ctctataact ccggatttcg tccgggaccc aacagctttt tcctccgttt cctggagaac
     3061 catgacgagg accgcgtcat ctaccgctat ggcagcggtg aaaagaccat cccggtctcg
     3121 acagccctct tcatggccac cggcaccccc ctgctctacg ccggccagga ggccggaatg
     3181 ggcctctacg gcggcaccct cgacgcccgc cgccgcggca ccatcaactg gtccggtctc
     3241 tacgccgcca tcctgacccc gcattatcaa aaactggccc agatccgcgc ccagttcccc
     3301 gccttccgca cccagttcga ggatagcaac agcgacggcc gcatcatcgg ccccgacgag
     3361 gatgtgcagc gccgcctcga ccagggcagc gccaccagct acgtcatggc ccggccctgg
     3421 ctcgacgcca acggcctggt ggtgatgaat ttcgccggca ccgcacagga gtttaccata
     3481 gccctcaagg ccggggagtg gatgcgtttc agcgccggat tttcagcgca ggggagctat
     3541 tggctcaaca acctctacga caataccagc cggccggtga gcggggcgga gctcgacacc
     3601 ctgaaggtga gcctgccacc ttatggcgtg gccatctaca ccatcgccac cgcagagcag
     3661 cggctggtgc tgcccgacct cgaggtgggc gttgagcaca agagcccggc ggaactgccg
     3721 caagcattcc acctcgccgg cagctggccc aatcccttca acagcgccac cgccatccgc
     3781 ttcaccctgc accaggcgga gcggatcgag gcggagatct ttgacgccct cggccggcgc
     3841 gtgcgcagcc tgactgcggc cgccctgccg gccggcgaac atgaactgca ctgggacgga
     3901 cgtaatcaga cgggaatgca ctgcccgagc ggcctctact tcctcaggct gcgccacggc
     3961 agccagaccg ccgtccacaa gatggccctg gtgcgctgac aggaggctgc agcggatgca
     4021 gcagcagatc agaaaaccgg gagggtgctg aagatgagtt atcaccgcct gctgaggcca
     4081 tgggccgcaa cgatgctgct catcctggcg gccctggcag gcgcccagac ctggaccgcc
     4141 gacaacggca acggttcttt caccaatccc cttttttatg aggagttctc cgatcccgat
     4201 atcattcgcg tcggggatga ctattacctc accggcacca ccatgcacgc catgccgggc
     4261 ctccccgttc tccattcgcg cgacctggtc aactggaagc tgctcggtta tgccgccgaa
     4321 cgcctcaacc tcggcccgga gctgcgcttc gaagagggcc gcgaagccta cggacagggc
     4381 atctgggccc cctgcttccg ctaccatgcg ggcacctttt acatctttgc caacgtcaac
     4441 ggccatggca cccagctcta taccgccaac actcccgccg gtccctggcg tcatcagacc
     4501 atgcgcgccg ccctccacga cgtttccgtg ctctttgatg atgacggcaa ggtctacgcc
     4561 gtctggggct acgacgagct gcacatggcc cagctgactc cagatctgct cgatatcgtg
     4621 cccgggaccg aaagagtggt cgtcgcgcgc ggcagcggcg ccggcgaggg ctgccacttt
     4681 tacaagattg acggccgcta cttcatcacc agcaccaatt acgatccggt ctgctaccag
     4741 gtctgcctgc gcgccgaggc tccctatggg ccctacgagc tgcgcgtgat cagcgcggag
     4801 gagaatctcg gcgtcggcac cggctggcgc ttatggaata cccgcgaggg gccgccgttc
     4861 gccctgcagc cgcccattga aaattttgtc ggctgcatcc ccatgcatca ggggggcatc
     4921 atccggatcc agtcgggtga atggtgggga tggtcgatgc tggaccacaa ttcggtcgga
     4981 cgcgtcaccg cgctctcccc ggtgacctgg gtggagggct ggcccctctt cggccttccg
     5041 ggcaacctga cccgcacccc gcgcacctgg ataaagccca acaccggttt ctcctccccg
     5101 ccttcagctc cctttgagcg caatgacgag ttcagcggcc ccgggctgaa aaacggctgg
     5161 cagtggaacc atcacccggt cgaggaaaaa tggtcactca ccgagcggca aggcttcctc
     5221 cgcctgcacg ccctgcccgc tgaagatttc tggcgcgccc gcaacagcct cacccagcgc
     5281 ggcatcggcc cggaatcgat cgccaccgcc gaactggatc ccgccggtct gcaagccggc
     5341 gatgtcgccg gtctggcgct gctcaacctg ccctgggcct ggatcggcat cgccaggctg
     5401 gaggagggaa tggaactgca gttctgcaac cagcaggcac tcgagctgct gcgcgtcccg
     5461 gtcacagcga agaaagtctg gctgcgcgcc cactgcaatt tcgataccga taccggcagg
     5521 ttcgcctaca gcctcgatgg cagccgcttt acggagatcg gcggggaaat catcatgccc
     5581 taccagctca agacctttca gggggtgcgc tatgcccttt tctgttatac gacgcgcgga
     5641 gaagagggcg gctgggccga tttcgaccgc ttcacagtga ccgagccgcg ctgccaggga
     5701 ctcacccgtc ccattcccca cggccggctc atcaccctca ccagtctggc cgacagcacc
     5761 gtgctggtca actggcgcaa tcatctccgg ccggccgagc gcaccagccc ctttgccgcc
     5821 ggggatgcct ccctcttcag ggtgctcgac cgcggccagg ggcgcatcgc cctgcagtcg
     5881 accgccacag gcggctacgt caccgtcaag ggcgaggccg ggctggccga ggtgcgcatc
     5941 gaggccgccg agcagggcga ggcctcactc tttcagtggg tggacatgct ccggggcgat
     6001 ctgatgctgc tgtccctgca cagtcatcgc tatctcttcg ccgatccgga ggcgcgcagt
     6061 ctctgttcgg ccgatgcccg cagcgcccgg cccgaccgca gggatggctc ctgcttcagc
     6121 tggcaactcg ctgcggaaaa tcagcaagcg cgggagtgaa ggagctcacc ctgagaaaag
     6181 gccgccaatg tgtcgatccg aaaaaaaggt ttccgacggg caaagtagaa attgcgcgcc
     6241 ggagtgctcc gggcgcagtg gatnnnnnnn nnnaggggga atcccttgct cttccgatcg
     6301 ctgccttcca gtcccgggcc gccagcgcgc cccgggctcc gggcgtctat ctgatgaagg
     6361 acggcgacgg ccgcatcctc tatgtcggca aatcaatcga tatccgcagc cgtctgcgcc
     6421 agcatgccgc atcgctgcgc gccggctatg gggagcgcaa ttaccgctgg atccggcagg
     6481 tccgcgagct ctcctggctg gagaccaact ctgaactcta cgccctgctg ctcgaggacc
     6541 agctcatcaa ggcgcactgg ccggcgggca atgtgcgcca gaaggagtat ctcgaatacg
     6601 cctggcttgc cttctccgta gaagccctgc cgcgtctgca ggtgatcgat gcccggcagc
     6661 gcggtcaatt cgacgccgtt ttcggcccct tccatgacct ctatcatgcc cgcgacatgg
     6721 ccgatctggt gaagatgcgc ttccgtctgc gcacctgcgc agcagtgcgc gcggagggat
     6781 gtttgcaggg cgagatgcgc aaatgttccg ccccctgccg cactgcggag gctgccgaac
     6841 gctatcgcgg caccgtcgcc cgcgcagccg catcgctaca gcgtcatgat ccctggttct
     6901 tccggttcat cgattacacc atcctccgct gcagccgcaa ccgggaattc gagagggcag
     6961 cctggctgca cgccatgcgt caccgctact gcgccctgat ccggcgccag gatttcatcg
     7021 aacatttcaa acgccatggt ctggttgtgc gggagggcgg gcgatgggag aatacatttt
     7081 actttctgca tggccggatg atccggcgcg aggggggaga gctcctggca ggggaggctc
     7141 cggcggagga aggagtattg agcgagtggc agatcatcga ccgcgcccag gtgatctggg
     7201 catggctgca gggagggcgg atttcaggcg cggcggctgt gatcgatgcc cgctttttcc
     7261 gcgcagagat agccgatagg taagagcatt accctgccgg cgcctgataa cgcgggatgc
     7321 cccaactgat gttcatggtg gagggagcga cggagcgcag ccagcgctcg ctcagagcgc
     7381 tgaggagagc cgccggaatg gaagcggccg ggcggaggcg gacgatggcc tccgcatcga
     7441 gagcagcgat ttgccggatc agctgcgccc tcacctcttc gagggtggcg gctgaaccat
     7501 cgacggtcag gtcgatcatc ggccgcgccg gcaagggcat gaagcgatga cgaatcccgc
     7561 cgccgggagc cagctcgatg atcagatagc ccttctcctc ctcccgctct gcaaaggagg
     7621 tgcgctcgat cgagccgggg taaaagaccg gcgcgttcag cctcctgccg gcgagatcgc
     7681 gggtcagcac ctggtggcga tggatgtggc cgctcaggac cgcatcaaat cccgccggaa
     7741 tatcccgccc cctgatcgcc tcctcgccgc tgcgaaaggt ataattctgc acccccacct
     7801 gcgccccttc caccgcctgg tgcatgcaca gcagccggat atcagccgag caatcacgcc
     7861 atccgctctt ctccacctca gggacgaaca ggcccgccgg attatgccga atgcagggta
     7921 gccccgtcac ggcaatgcga aggccgcgca aggtcaggac gagggtgagg ggatggtcaa
     7981 agatggcaat ctgcggatgg ctttcgagta gcgagcaggg aatgttggag cgctcgtgat
     8041 tgcccggtac aatgcagacc gggacaccga gatcggcaat ctgcagcagg ggcgcaaaag
     8101 ccgattcgat gatcgacgga tggacccggc tgcggaaaaa catgtcgccg ccatggacaa
     8161 cgaggtcgac ttttccagcg gctgccggct gcagggcggc tgtgtagttg gcgtaaaaat
     8221 catccccccg cctccgcctc tggatgcggg gccgcagcgg ggtatcgatg ccgagatggg
     8281 tgtcggcgag gaagaggatc cggatcaatt cccctgctcc ctctgcacca catgctcgag
     8341 gtagaacttt ttaaaggcct catggatttc gtcgcgcacc ctgcggaaag cggccagctt
     8401 ttcctcttca gtgccctcaa ccgctgcagg gtcatcaaag ccgatgtgca gctcctgctt
     8461 gacctcgccg aggaagaccg gacagctctc gcgggcgttg tcacagactg tgataacgta
     8521 gtggaagggc agatgggtaa agatcgcgac cgatttggga cgggcttgcg agatatcgat
     8581 gtgcttctcg gccatcacct caacggcgag gggatggacc gtggcggccg gttcagtgcc
     8641 ggcggattcg acctcgaggg cgggattgaa atgcttcagg aaggcctcgg ccatctggct
     8701 gcggcaggaa ttgccggtgc agaggatcag gatgcgcatg acgggatctc cttcaggata
     8761 gtgtcgtttg caggtgccgg gcgaacgcca gtgcctgttt aaggtaagag aaaaaatcat
     8821 caaatgcaag cggttgggaa gcagggtacc cccgaaatct ggctcttacc cgacacctgg
     8881 cgccgcttgc ccccaccccc ctgctaattc cagcataccc tcggcctgtg ccctggctga
     8941 ggattctttt tacatgcgtt ttctcacctg cagcccatta tttttttcat caatagcatc
     9001 cgggttaaac tcggattttc atgggtatca aggttttgca gggagaagcg agtggtgaaa
     9061 atagtggata aagtacttga cattctgcct cgaaatggtt aggttacaaa agatatcaga
     9121 tataacatgt catacaagta gccaggggac tgctgtggaa gaactcttcg gaaaaatcgg
     9181 aaaaagcctt accctaagtc aggagatcga actgcgcatc gaggattcga tacgccagaa
     9241 aaagctcgag cccggccaga aactgcccag cgagaaggaa ctcggggtga tctttggcgt
     9301 cagtcgtaca gcggtgcgcg aagccctgcg catgctgagc gcgcgaggac tgctccatgt
     9361 ccgcaagggc gacggcgtct atgtcaactc cttctcctcc tcgctcgcca gcaaatccat
     9421 gagcctctat ctcgagctgc aattcgacac cgattcgatt cttcatctgg tccatctccg
     9481 tcagcttatc gagcccaacc tcgcccgtct ggccgcccaa aagcgcagcg aggaagattt
     9541 ggttcgcttc aggctaaatc ttgccgcttt ctccgaccgc acccaggacg ccgaccagct
     9601 cgcccgtctg gatgtcgagt ttcataccct tatcgtcacc tccagtcaca atcctctcat
     9661 cccgctcatc atggatccca ttttcagcat gctcccccgc gtcaagactc tgatcgtcaa
     9721 tcatctgcaa aagactcata gcgataacgc ccccaactac catcagaaaa tatatgacat
     9781 gatccgcctc caggatggag agggcgcctt ttcggcgatg caggaacatc tgaaaatcgc
     9841 tgaacgggac tccctcctgc tcgcccaatg gatgaaagag aaccagg
//
LOCUS       DAOPWU010000105         9883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6901_length_9883_cov_4.598087, whole genome shotgun sequence.
ACCESSION   DAOPWU010000105 DAOPWU010000000
VERSION     DAOPWU010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9883
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6901_length_9883_cov_4.598087"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PKM23_07050"
     CDS             <1..731
                     /locus_tag="PKM23_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNY91269.1"
                     /translation="RISGSKSFEYVLVPRFPGEQRIRPIVFSYFDLAARRYATATTPE
                     IVIPVAKGSGEVTVLPSGLSKEEVALINQDIRFIKLNAPEWRRKGGAFHRSGWFTLLW
                     ILPLLALGAALGYRRHLDKLSGNIAYARSRRANRMAMKRLSAASGLLQVEKQKEYFAE
                     ISRALLGFAADKLNLSEAGILSSELESRLRERGVAEGTIGEYLQLLKVCDYQRFAPSE
                     VTLNQMQGIYKEAKEAIINLEKAV"
     gene            741..1490
                     /locus_tag="PKM23_07055"
     CDS             741..1490
                     /locus_tag="PKM23_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY91270.1"
                     /translation="MRLIISLILLAAVAFASQSAMQYYQQGNTAMQNNAYEEAIKAYE
                     SALATGYESAELYYNLGNAYFRMQELGKSILNYERARALKPADPDIATNLELAQLRVV
                     DKIAAPPALFTARIWTAVKQALSVGQWGVLALILFNLLIAAVIVRLLVSHSGVRAVAG
                     YLFIPLLVLFLVAGTLYWATAREAKNVRFGIIMPHKVSVLSAPASDATQAFDLHEGTR
                     VRIAEPSGAYVKVILLDGKVGWMTRDGVEEI"
     gene            1820..3169
                     /locus_tag="PKM23_07060"
     CDS             1820..3169
                     /locus_tag="PKM23_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91271.1"
                     /translation="MKTVKYKPVLLTLMTVVLAFAFTGCYTQLAKAPERVYYDEPYDE
                     EVEEPVESEYTYDDDDGYGLETPDTIIVDRSAYPDIYVQDWVPTVYIDAWWYSPYWHY
                     RTNSWWRWHHRWDPWYGYNDYWGDPWYWPSFSYYGGWGFYDPWYGYAGYWGSPYYYHH
                     HNGWGWGGGGWGHEGQYRDWERRSFGVGNYAVSKRPRTVGSSDQAGAPAETRRSGGYE
                     RRPGDAASRTRTTDGTTPAGTPDTRRAPTRRSGESGTVTRPSTGDKRTTPSGEIRRKS
                     TGSESSGTSGSTRREKSGSEVRRSTNSGNSSGEKRSQPGLNRTPSRPQQTPQIERKSS
                     EKKSSSGKRSEASNRSSSQRMRSDYRRSTESASRAESGRSYSSQRRSSSTPSARSSEG
                     RSSSRSSGYSRSSGSSYSSTPARSSGSSSSGGSSSRSSSSGSSSSRGSSSSGGSHRR"
     gene            3265..4602
                     /locus_tag="PKM23_07065"
     CDS             3265..4602
                     /locus_tag="PKM23_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91272.1"
                     /translation="MNKTLIMATLVMGIGLASGAYAQSNVLTEDFFHMGNDLGVGARA
                     LGLGGAYTAISDDYTAMYWNPAGIGQMKRREFNIGFSQNSIKSDALFLGTGAETADSF
                     TRLNSIGLVFPVPTARGSLVFGVGYDKGRDYDAMVDVGALTIDSLYQTNSILDEGSRN
                     QFTLSGSIEVSPNLFMGAGINFINGHIDHSSEFMNEDKYDLYFSPGDEADPTDDADWS
                     YYKQHQTLGSDIDGTNLKLGLLYRSGSLWRLGASVTLPTTYTITENWGWSETEFYDAE
                     HDAWYDEDEGTYKYQYTEPYAASVGFSAKLLDLLLLSGDAEFRDWTQAKFEDDPPISG
                     MERTAANRAIKKEMQAVTRLRAGAEISVPATNLRLRGGLFSDPSPYKDSVIRPDRTYY
                     SGGLSFMMDKQAMLDLTYVRGSWEESRSDDLSDGPITMDRSLQKIIATLSLRF"
     gene            4816..5550
                     /locus_tag="PKM23_07070"
     CDS             4816..5550
                     /locus_tag="PKM23_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNY91273.1"
                     /translation="MASQIPLDYPLSAQLNRLEGEVGLTVFVRPDGKPEEVTLSRSSG
                     HEVLDEAALRFVKKLDFTPGTLDEVPVGAWTRLVLRYKLSEQAFERERWLADIFELQK
                     QIDAERDPDLRETLLRRLYTHYVGCVTYVDSNDDPAINDLIRSAVGKAREEHWRPFWN
                     QYASPFVLFDDFLANYADSEIASQARQDLARYLLDMETKIRMKSLKSRRVAQSSGGLV
                     ELLEERLQALQQELRGEVQPLPAAPQ"
     gene            5682..6269
                     /locus_tag="PKM23_07075"
     CDS             5682..6269
                     /locus_tag="PKM23_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91274.1"
                     /translation="MKRAVVVLMLLFCSFPLMAQRKGSTEIGGSISFHSNQDAQQRNY
                     SDLGFDGLICYYLSRNIGIDIEPGVGISFQPDSVSIATLLMGSLRMRLFDLIPSGYRK
                     NDVYRLDLGVSSSVFANLGVGLWSEGYSLSQKPSTTYSGPAVMAGIGTFSQFGRFSTL
                     RVKLQFIELFPSGPVFTKRRTLVQVGVGFGLFIRS"
     gene            6350..7333
                     /locus_tag="PKM23_07080"
     CDS             6350..7333
                     /locus_tag="PKM23_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91275.1"
                     /translation="MRKYTFLLLALVCLTAPVQAQLSKREINRRDSSRPGSREDYFIN
                     TLQLIDLPTASLLKGGNVSGSLLLFEEGGMLARLSAGISERMMFGISYGADYIIGDQV
                     IKWNDAPAVHIAYRAVDESARFFSVVFGLDTQGYGKYWRDSDYPDALPGQIDPTKVPY
                     KRYTYKSRGFYAVASKNYRAFWNVGLHVGANYSLETSDKDRFPDLFCGMDWQLGRDLA
                     VVAEYSFAFNNDEIPMPAGSNGLVRNNFGSKGFLNAGVRWAFQPNMFLEFAAKNLLAD
                     NLGNRDYVRVLKIVYFTRATDRDTRYTQSRKLSIWERIFGSRRRDDNSRDR"
     gene            7379..7918
                     /locus_tag="PKM23_07085"
     CDS             7379..7918
                     /locus_tag="PKM23_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91276.1"
                     /translation="MKFYRLTTLFLLAAVLAFCGCYTTIRLPRASAEEEEYVEEWRRM
                     PLNPSIPASHAGDFDWLFYYQLPWWLDAAEVYYDQSPGQSWAPEEFRQRYPQSPDYSG
                     SFSGSYSPTVTSPGLGKQAADSSTSSGASEQKDSRRSFNQNAGSSPAAGTSESSKESG
                     SDRRDSKPSSSGTEKPKRR"
     gene            7996..>9883
                     /locus_tag="PKM23_07090"
     CDS             7996..>9883
                     /locus_tag="PKM23_07090"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNY91277.1"
                     /translation="MSLKQRFIFILVIILLCAAAFILFGYLSVRRSVPPESGSFPAPG
                     LERQVEIGRDEWGVVHIDAGSEYDLYFAAGYSCAQDRLWEMDFLRRAATGRLAEILGP
                     ELVEVDLFTRTIGFGRLGRRLLPALPSATAANLRAYCAGINTYIATARQLPLEFGALR
                     YTPEPWQPEESLACMRLIGWLLSMGWNIDLVYAETAAKVDSQKFVRILPEALPGLRSF
                     PLGVDESDSTAAAPLKRRSRMDRFLRSLLPLPSLDSLRRTPLLEERKRDSRSGEPVSA
                     VLRQGEEELRRLFNSPSGGLGSNAWVINGRLTERGQALLANDTHLVFTVPSLYYLMHL
                     HSPAVNAVGAAFPGLPGLVVGRNEFVGWGITNGMVDDVDLVRLEPDSIDAEYYRFGRQ
                     RYRYIYHDETIAIRGGEEKKVRIPWSHIGPVISAATPILGYRGEDPLVLRWSGFENDD
                     PLTAFQELMTAKDWGGFLAALESSKNPGENFFYADRAGQIGYKLAAAVPRRTYRDAIV
                     PLPERTAVPDSLASPDSLAREARVRDWLGFIPFTQLPQLFQPPSGWIANANNCLVDTT
                     YAHFISAYWEPDYRYNRIKAALDTLSRWNLASCRALQADLYSGHAAAFVPLLMKAIQH
                     LDLPR"
BASE COUNT         1907 a         3161 c         2818 g         1997 t
ORIGIN      
        1 accgcatcag cggctcgaag agctttgagt atgtgctcgt gccccgcttt ccgggcgagc
       61 agcgcatccg gccgatcgtc ttcagctatt tcgacctcgc cgccagacgc tacgccacgg
      121 ctacgacccc cgagatcgtc atcccggtgg ccaaggggag cggcgaagtc acggtgctgc
      181 cctccggtct gagcaaggag gaggtcgcgc tcatcaacca ggatatccgt ttcatcaagc
      241 tcaacgctcc agaatggcgg cgcaagggcg gcgccttcca ccgcagcggc tggttcaccc
      301 tgctctggat cctgcccctg ctggccctgg gtgcggccct gggctatcgc cgccacctcg
      361 acaagctcag cggcaacatt gcgtacgccc gcagccgccg cgctaaccgc atggcgatga
      421 agcggctgag cgcggcctcc gggctgctgc aggtggagaa gcagaaagaa tactttgccg
      481 agatctcccg ggcgctgctc ggcttcgccg cggacaagct caacctgtcc gaggcgggca
      541 tcctcagcag cgagctggag tcgcgcctca gggagagggg tgtggccgag gggacgatcg
      601 gagagtattt acagctgtta aaggtgtgcg attatcagcg cttcgccccc tcggaggtca
      661 ccctgaacca gatgcagggg atctacaagg aggccaagga ggcgatcatc aatcttgaaa
      721 aagcggtcta gcctatgcga atgcgattaa tcatttccct gattctgctg gctgctgtgg
      781 cctttgccag tcagagcgcc atgcagtact accagcaggg caatacagcc atgcagaaca
      841 acgcctatga ggaggcgatc aaggcctatg aatcggccct tgccaccggc tatgagagcg
      901 ccgagctcta ctataatctc ggcaacgcct attttcgcat gcaggagctg ggcaagtcca
      961 ttctcaacta cgagcgggcc cgggcgctca agcccgcaga tccggacatc gccaccaatc
     1021 tcgaactggc gcagctgcgg gtggtggaca agatcgcggc cccgcccgcc cttttcaccg
     1081 cgcggatctg gaccgctgtc aagcaggcgc tgagcgtcgg acagtggggg gtgctggcgc
     1141 tgattctctt caatctgctc atcgccgccg tcatcgtccg gctgctggtt tcacatagcg
     1201 gcgtgcgcgc ggttgctggc tatctattta tcccactgct ggttcttttc ctcgtggccg
     1261 ggaccctcta ttgggccact gcgcgggagg cgaagaacgt ccgctttggc atcatcatgc
     1321 cccacaaggt cagcgtactg agcgccccgg ccagcgatgc cactcaggcc ttcgatctcc
     1381 acgaagggac cagggtgcgg atcgccgagc cctccggggc ctacgtcaag gtgattttgc
     1441 tggacggcaa ggtcggctgg atgaccaggg atggggtgga ggagatatag cttctccacc
     1501 gccgggtcag gcagtatcag ctatcgataa tggtgagaca gcttgacgat gattttgccc
     1561 tctgcaaagt aaagccgcag ctccctgttt atcttcaggg cccggtcgtt taatccctcc
     1621 cggcaccaca gcatagcctc aactgtcccc ctgcggcgat tttgtgcacg aaaacggaac
     1681 atttcatttt aatcaccgtt agatattatg caatgagcac aagggccaat tgtgaagaaa
     1741 atactttgca tttggctact gcaagtgcta cattgagaag aagatagaga tttatccgcg
     1801 ccttgtttta ggaggcccca tgaaaactgt taaatacaag cctgtactcc tcacactcat
     1861 gaccgtcgtt ctggcgttcg ctttcaccgg ctgctacacc caactggcca aggccccgga
     1921 acgggtctat tacgatgagc cctacgacga agaggtagag gagccggtcg aatcggaata
     1981 cacctatgac gacgatgacg gctacggcct ggagacgccg gacaccatca tcgtggaccg
     2041 gtcggcctac cccgatatct acgtccagga ttgggtgcct acggtctata tcgatgcctg
     2101 gtggtattca ccctactggc attaccggac caactcctgg tggcgctggc atcaccgctg
     2161 ggatccgtgg tacggctaca acgattactg gggcgatccc tggtactggc cctccttcag
     2221 ttattacggc ggatggggtt tttatgatcc ctggtacggc tatgccggct attggggcag
     2281 cccctactat taccaccacc ataacggctg gggttgggga ggcggcggct gggggcatga
     2341 aggccaatat cgtgactggg agcgccgttc cttcggcgtc ggcaactatg ccgtcagcaa
     2401 acggccgcgc acggtcggtt cctccgatca ggccggcgca ccggccgaaa cgaggcgttc
     2461 cggcggctat gagcgcaggc cgggcgacgc agccagccgc acacgcacga ctgatggcac
     2521 tactccggca ggaacccccg atacccgccg cgcaccgacg cgccgcagcg gcgaaagcgg
     2581 cacggtgacg cgcccctcca cgggcgacaa gaggaccaca ccatctggtg aaatccgccg
     2641 caaatcaacc ggaagcgaat cgagtggaac ctccggctcc acccgacgcg agaagagcgg
     2701 ctccgaagtg cgccgctcca ccaattcagg gaattcgagc ggagaaaagc gcagccagcc
     2761 cggactgaac cgcacgccaa gccggccaca gcagaccccg cagatcgaac gcaaaagttc
     2821 ggagaaaaag tccagctccg ggaagcgcag cgaggcctcc aatcgctcga gcagccagcg
     2881 gatgcgttct gactaccgcc gttccacgga aagcgcctcc cgcgccgagt ccggccgcag
     2941 ctactcctcg cagcgcagga gcagcagcac cccgtccgcg cgcagttccg agggccgttc
     3001 cagctcgcgt tccagcggct attcgcgcag cagcggcagc agctactctt caactccggc
     3061 gcgcagttca ggcagcagct cctcgggcgg cagctcttcg cgcagcagct cctccgggag
     3121 cagctcctcg cgcggcagtt cctccagcgg tggttcgcac cggcgctgag cctggtaagt
     3181 ccagtcgctc ctttccgacg tgacggagat atggcttttt tacgactgca ttcgatactt
     3241 ttgggagatc ttgaaatgag gattttgaac aagacgctga tcatggcgac gctggtgatg
     3301 ggtatcggcc tggcctccgg cgcatacgca cagagcaatg tgctcaccga agattttttt
     3361 catatgggga atgatctggg cgtcggcgca cgcgccctcg ggctgggcgg cgcctacacc
     3421 gcgatctcgg atgactatac cgccatgtac tggaaccccg ctggcattgg acagatgaag
     3481 cgccgggaat tcaacatcgg cttttcgcag aacagcatca agtccgatgc cctctttctc
     3541 ggcaccggag cggagacggc cgacagcttc acacgcctga actccatcgg tctggtcttt
     3601 cccgtgccga ctgcgcgcgg cagcctggtc ttcggcgtcg gctacgacaa ggggcgcgat
     3661 tacgatgcca tggtggatgt gggcgccctg accatcgact ctctctatca gaccaattcc
     3721 atcctcgatg agggcagccg caaccagttc accctttcgg gttccatcga ggtcagcccc
     3781 aatctcttca tgggtgccgg catcaatttc atcaacggac atatcgacca ctcctcggag
     3841 ttcatgaatg aggataaata cgatctctat ttttctcccg gggatgaggc cgatccgacc
     3901 gacgatgcgg actggagcta ttacaagcag catcagacgc tcggctccga tatcgacggc
     3961 accaatctca aactgggtct gctctatcgc tccggaagcc tctggcgcct gggcgcaagc
     4021 gtaaccctgc cgaccaccta taccatcacc gaaaactggg gctggagtga aaccgaattc
     4081 tatgacgccg agcacgatgc ctggtacgat gaggatgaag gcacctacaa ataccagtac
     4141 accgagccct atgccgcctc cgtcggcttt tccgccaagc tccttgacct cctgcttctc
     4201 tccggtgatg ctgaattccg ggactggacc caggccaagt tcgaggatga tccccccatc
     4261 tccggcatgg aacggacagc cgccaatcgc gccatcaaaa aggagatgca ggctgtgacc
     4321 cggctgcgcg ccggggctga aatcagcgta cctgccacca acttgcgcct gcgcggcggc
     4381 cttttcagtg atccttcgcc ctacaaggac agcgtcatcc gccccgaccg cacctattac
     4441 tccggcgggc tcagttttat gatggacaaa caggccatgc tcgacctgac ttatgtccgc
     4501 ggcagctggg aggagagcag aagtgatgac ctctcggatg ggccgatcac aatggatcgc
     4561 tccctgcaaa aaattatcgc caccctctcg ctgcgcttct aacccggccg gatgcgccgg
     4621 gacgggaggt gttgcgatgc ggcattccgg cctctttcgt cgtccggatg gcatgggaag
     4681 gggagtgacc tgttcactcc cctttttcaa ttccaaggag ctcttatgca agtcggtaga
     4741 gcattatgga tattgctttt aattgcggtt ttcgggttcg gctgcgcatc ccattcccgc
     4801 cttaaaccgc cgcgcgtggc atcgcagatc cccctcgact atcccctcag cgcacagttg
     4861 aacaggctcg agggggaggt cggcctgacc gtctttgtcc gccctgacgg caaacctgag
     4921 gaggtcaccc tgagccgctc atccggccac gaggttctgg atgaagcggc cctgcgcttc
     4981 gtcaaaaagc tcgatttcac gccgggcacc ctggatgaag tgccggtcgg ggcctggacc
     5041 cggctggtgc tgcgctacaa gctctcggag caggctttcg aaagggagcg ctggctcgcc
     5101 gacatcttcg agctgcaaaa gcagatcgac gctgaacgcg atcccgatct gcgcgagacc
     5161 ctgctgcgcc gtctctatac ccattatgtc gggtgtgtca cctatgtcga cagcaacgac
     5221 gaccctgcga tcaatgacct catccgcagc gccgtcggca aggcgcggga ggagcactgg
     5281 cgtcccttct ggaatcagta tgcttccccc tttgtgttgt tcgatgattt tcttgccaac
     5341 tatgccgatt cggagatcgc cagtcaggcg cgtcaggacc tggcccgcta tctgttggat
     5401 atggagacga aaatccgtat gaaatcgctc aagtcccgcc gcgtggccca gtcgtccggc
     5461 gggttggtgg agctgctgga ggagcgtctc caggccctgc aacaggagct gcggggtgag
     5521 gtccaacctc tcccggcggc gccacaatga gattcaatcc atgtgtagaa aagcagccgg
     5581 atcccgcatt ccactccgac cggttgtaag agccggccag gtgcaaatcg tggtaccgta
     5641 acagcagatg aatcagttcc tgggatagaa tgggaggtca tgtgaaacgt gcggttgtgg
     5701 tgctgatgct tctcttttgt tcgttccccc tcatggcgca gaggaaaggc tcgacggaaa
     5761 ttggtggttc catctccttt cattcaaacc aggatgccca gcagcggaac tatagcgatc
     5821 tgggttttga cggtctgatc tgctactatt tgagccgcaa catcggcatc gatatcgaac
     5881 ccggtgtcgg catcagtttt caacccgatt cagtgagcat tgcaacgctg ctgatgggca
     5941 gcttacgcat gcggctcttt gatctgattc cctccggcta tcgcaagaac gatgtctacc
     6001 ggttggatct gggagtcagt tcctcggtct tcgccaacct cggtgtcggt ctctggtcgg
     6061 agggttattc cctgagccaa aagcccagca ccacctactc gggcccggcg gtcatggctg
     6121 gcatcggcac cttttcccag ttcggccgtt tttcaaccct gcgcgtcaag ctgcagttca
     6181 tcgagctctt tcccagtgga ccggtcttca caaagagacg gacactcgtc caggtcggcg
     6241 tcggattcgg gctctttatc cgctcctgag gcatcgggtt ttatcgttga ctttctgagg
     6301 ccttttcatt atatttaata aatttggaaa cggctaaagg gccatcccta tgcgcaaata
     6361 taccttcctg cttctcgccc tggtctgcct gaccgcgccg gtgcaggccc agctctccaa
     6421 gcgcgagatc aacagacggg attcttcccg gccgggcagc agagaggact atttcatcaa
     6481 caccctgcag ctgatcgacc tgccgacggc cagcctcctc aaggggggca atgtcagcgg
     6541 cagcctcctc ctcttcgagg agggcggcat gcttgcccgc ctttcggccg gaatctcgga
     6601 aaggatgatg ttcggcatct cctacggcgc cgattacatc atcggggatc aggtgatcaa
     6661 gtggaatgac gcccccgccg tccacatcgc ctaccgggct gtcgacgaga gcgcgcgctt
     6721 tttttcggtc gttttcggac tcgataccca gggttacggc aaatactggc gggattccga
     6781 ttaccccgat gccctcccag gccagattga tcccaccaag gtcccctaca aacgctatac
     6841 ctacaaatcc cgcggctttt atgccgtcgc ttccaagaat tatcgggcct tctggaacgt
     6901 cggtctgcac gtcggcgcca actacagcct ggagaccagc gacaaagatc gtttccccga
     6961 tcttttttgc gggatggatt ggcagctggg tcgcgacctc gccgtggtcg ccgaatattc
     7021 cttcgccttc aacaacgacg agatcccgat gcctgctggc agcaatggtc tcgtcagaaa
     7081 taatttcggc agcaagggtt ttcttaatgc cggcgtacgc tgggccttcc agcccaacat
     7141 gttcctcgaa ttcgccgcca aaaacctgct cgccgacaac ctgggcaacc gggactatgt
     7201 ccgtgtcctc aaaatcgttt atttcacccg cgccaccgat cgcgatacga gatacaccca
     7261 gtcgaggaag ctttcgatct gggagcgtat tttcggcagc cggagaagag atgacaactc
     7321 tcgcgaccgc taatcggccg ggaaaaccag accagacaag ttctctttct ggagtaaaat
     7381 gaaattctat cgcctgacaa ccctgttttt gctcgccgcg gtgctcgcct tctgcggctg
     7441 ctataccacc atacgcctgc cgcgcgcttc ggccgaggag gaggagtatg tggaggagtg
     7501 gcggcggatg ccgctcaatc cctccatccc cgcctcccat gccggcgatt tcgactggct
     7561 tttctactat cagctgccct ggtggctgga tgcggctgaa gtctattacg accagtcgcc
     7621 tggccagtcc tgggccccgg aggaattccg gcagcgctat ccccagtcgc ccgactactc
     7681 gggcagtttt tccggcagct actccccgac ggtgacctcc cccgggctcg gcaagcaagc
     7741 ggccgacagc agcacctctt caggggcgag cgagcagaag gattcccgcc ggagctttaa
     7801 ccagaatgcc ggatcctcac ctgcagcggg gacatcggag agcagcaagg aatccggctc
     7861 cgatcggcgt gattccaaac cctccagcag cggaaccgag aaacccaaaa ggcgctgatt
     7921 cctgcacgtc gggagcacgg tgtcctgaag gcaagagttc ccctctcttg cctttttcat
     7981 taatggaaaa gagcgatgtc cctcaagcag cggttcattt tcatcctggt cattatcctg
     8041 ctctgtgcag cggcctttat ccttttcggc tatctctccg tacgccgctc cgtcccgccg
     8101 gagagcggct catttcccgc ccccggtctg gagcggcagg ttgagatcgg ccgggatgaa
     8161 tggggcgtcg tccacatcga tgccggcagc gagtatgatc tctatttcgc cgccggttac
     8221 agctgcgcac aggaccggct ctgggagatg gattttctcc gccgcgccgc caccggccgc
     8281 ctggccgaaa ttctggggcc tgaactggtc gaggtcgatc tctttacacg caccatcggc
     8341 ttcggccgcc ttggccgccg gctgctgccg gcgctcccct ccgccacagc cgccaacctg
     8401 cgcgcctatt gcgccggcat caatacctat atcgccaccg cccggcagct gccgctcgaa
     8461 ttcggcgctc tccgctatac tcctgagccc tggcagccgg aggagagcct ggcctgcatg
     8521 cgcctcatcg gctggctgct cagcatgggc tggaatatcg atctggtcta cgccgaaacc
     8581 gctgcgaaag tggactcaca aaagttcgtc cgcatcctcc cggaggccct gccggggctg
     8641 cgctctttcc cgctcggggt ggatgagagc gactcgaccg cggcagcgcc tctcaaacgc
     8701 cgttcgcgca tggaccgctt cctgcgcagc ctgctgccgc tgccatccct tgattctctg
     8761 cggcggactc cactcctcga ggagagaaaa cgggattccc gcagcggtga gccggtgagc
     8821 gcagtgctcc ggcagggtga agaggagctg cggcgcctct tcaacagccc gtcgggtggg
     8881 ctcggcagca atgcctgggt gataaacggc cgtctgaccg agcggggtca ggccctcctc
     8941 gccaacgaca cccatctcgt cttcaccgtg ccgtcgctct actacctgat gcatcttcac
     9001 tccccggccg tcaatgccgt cggcgccgcc ttccccgggc tccccggcct cgtggtcggc
     9061 cgcaacgagt tcgtcggctg gggcatcacc aacggcatgg ttgacgacgt cgatctggtc
     9121 cgcctcgagc ctgacagcat tgatgcggag tattaccgct tcggacggca gcgctaccgc
     9181 tacatttacc atgatgagac catcgccatc cgcggcggag aggaaaaaaa ggtccgcatc
     9241 ccctggagcc atatcggccc tgtcatcagc gccgccaccc cgatccttgg ctaccgcggg
     9301 gaggatcccc tggtcctgcg ctggagcggt tttgaaaacg acgaccccct gaccgcattc
     9361 caggagctga tgaccgcaaa ggattggggc ggtttcctcg ccgccctcga gagcagcaag
     9421 aatccgggtg agaacttttt ctacgccgac cgcgccggcc agatcggcta caagctggcg
     9481 gcggccgtgc ccaggcgcac ctaccgggat gccatcgtac ccctgccgga gcggacggct
     9541 gtgcccgaca gcctcgcctc ccccgacagt ctggcacggg aagccagggt acgcgattgg
     9601 ctcggtttca tccccttcac ccagctgccg cagctctttc agcccccctc cggctggatc
     9661 gccaatgcca ataactgctt ggtcgatacc acctacgctc acttcatctc agcctactgg
     9721 gagcccgatt accgctacaa ccgcatcaag gcggcgctcg acaccctgtc gcgctggaat
     9781 ctcgcctcct gccgcgccct gcaggcggat ctctactccg gccacgcagc cgcctttgta
     9841 cccctgctca tgaaggccat ccagcatctc gacctgccca gaa
//
LOCUS       DAOPWU010000106         9834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6944_length_9834_cov_5.681767, whole genome shotgun sequence.
ACCESSION   DAOPWU010000106 DAOPWU010000000
VERSION     DAOPWU010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6944_length_9834_cov_5.681767"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKM23_07095"
     CDS             complement(<1..374)
                     /locus_tag="PKM23_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HNY91278.1"
                     /translation="MINLFNKLVIFFLPIIPKFIVRMVAKPYVAGPTLEDGIRVVKEL
                     NSKGISATMDVLGESARKLEECQEAIDDYFKVLDRIAADKLDANISVKLTQLGLTLDR
                     EQCYQNIRKIVDKADRLGIFVR"
     gene            608..1372
                     /locus_tag="PKM23_07100"
     CDS             608..1372
                     /locus_tag="PKM23_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNY91279.1"
                     /translation="MGKIITIANQKGGVGKTTTAVNLAAALAVAEYPTLLVDMDPQAN
                     STSGYGYDQRALQATIYDAIINERPAQSLLLDTQLDYLKLLPSSISLVGAEVELVSAI
                     SREQRLARALEPVRDQFKYILIDCPPSLGLLTINSLTASDSVLIPIQCEYYALEGLGQ
                     LLNTIRLVQKHLNEHLAIEGVLLTMYDARLNLSRQVAEDVRRYFEGRVFETIVTRNVR
                     LSEAPSFGKPIILYDAVCSGSENYMSLAEEIIKNGE"
     gene            1362..2228
                     /locus_tag="PKM23_07105"
     CDS             1362..2228
                     /locus_tag="PKM23_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNY91280.1"
                     /translation="MANKKALGRGLRALIPDIPAEQDAPVQGKGIDFLDVDKIAPNPF
                     QPREKFDPESLAELKQSIAEKGLVQPVTVRKHGAGYQLISGERRLRAVRDLGLQKIPA
                     YILEVETDEEMLELAIIENIHREDLNAIDIAHGYKRLIDECKLTQEEVAVKVGKDRTS
                     VANFLRLLKLPRRIQESVQSSEISFGHARALLSLASSEDQLEMWKKIVGGSLSVRKVE
                     EMVREPEKGKAKPAKVTAAPNYYMEELENKLRLHLATKVELKSRNRGGAIEIEYYSSD
                     ELERIIALILGE"
     gene            2251..3150
                     /locus_tag="PKM23_07110"
     CDS             2251..3150
                     /locus_tag="PKM23_07110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNY91281.1"
                     /translation="MFGRKKAEGTARSHRRSLQILVIPDDQAEPRHYQISMRKFRILR
                     VAAVVLALHLFLGIVAYLQWARLAVRYDDLSKINGQLVENSKKVYELAAVFQKIEATD
                     AKLRSALGLDENRGSATLASDEVFEPPRRALPVEPILEPRRERPKPVEVNLGEKLGFL
                     QQSREIGIHEYLRSLPTYLPVAGVLTRNYSDEGAAHTGIDIAAARGSFISAAGDGVVV
                     FSGWTQDLGNMVIIYHGDGFFTYYGHNQRNLASRSTLVRKGDHIALLGSSGESTAPHL
                     HFELWKDGIALNPADYILAFARM"
     gene            3192..3587
                     /locus_tag="PKM23_07115"
     CDS             3192..3587
                     /locus_tag="PKM23_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNY91282.1"
                     /translation="MKSTDKPGELGTILGKGAVVDGNIKVEHSLRVDGKVVGDVTSGD
                     TLIVGAEGEIKGNVKVKNLVLGGRINGSVLSPGRTVLEARSELRGELKTGKLVIDEGA
                     IFDGKCSMSDAKAETGRTPGEADGETIIK"
     gene            3647..3844
                     /locus_tag="PKM23_07120"
     CDS             3647..3844
                     /locus_tag="PKM23_07120"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNY91283.1"
                     /translation="MNLGMQLAVAVILGTALGYWLDSKLGTSPLFLLLGVIAGGTAGF
                     MNVYKTVYPDRRDKESSTKQR"
     gene            3841..4248
                     /locus_tag="PKM23_07125"
     CDS             3841..4248
                     /locus_tag="PKM23_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91284.1"
                     /translation="MKSPMRIWRLVGWTVPAFLVLLIPLHYYGRPGMTSSYTLGYLVS
                     LFNIVFSLVSIQWAFRRKVKTFYAVVLGGMALRFILLAVMVYLVIAVLHWPLAGFLVS
                     FVLFFLVLQYFEIRYINSELKGRSSANHDHTDS"
     gene            4229..5059
                     /gene="atpB"
                     /locus_tag="PKM23_07130"
     CDS             4229..5059
                     /gene="atpB"
                     /locus_tag="PKM23_07130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNY91285.1"
                     /translation="MITQIVEHASEHGEAVQQAAEHAAEHGESGVGFIMHHIMAHPVI
                     KLPTVYGIDLSVTNHVLWMWIVSALLILGFYVAFRKPKLVPHGLANALESLVVFIRDD
                     VIMPNLGHEARAYAPYLLTAFFFIMLCNLAGLVPYGATATGNIGVTATLALFTFFVVQ
                     IAGVRKHGFLGYLSTFSPPGIPFLLKFIMIPVEVISMITKHFALAIRLFANMIAGHIT
                     ILALLSVIFLFKNWLVSPFPLAIILFSAILEVLISLIQAYVFTILSAVFISASVAEGH
                     "
     gene            5131..5358
                     /gene="atpE"
                     /locus_tag="PKM23_07135"
     CDS             5131..5358
                     /gene="atpE"
                     /locus_tag="PKM23_07135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNY91286.1"
                     /translation="MNPTAIAYLAAGFGSAAVTFSAAFGISKLASAAISGIARQPEAA
                     GDIRGAMIITAAMIEGVALLSVVICLLLGFK"
     gene            5379..5873
                     /gene="atpF"
                     /locus_tag="PKM23_07140"
     CDS             5379..5873
                     /gene="atpF"
                     /locus_tag="PKM23_07140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNY91287.1"
                     /translation="MELMTPAGGTIFWTTLTFLLLLFALTKVGWKPILSMLDEREKKL
                     KESLELAEQAKIASQKTLAEQNQLLETARREAQEILARNRKAAEATKEEIIKKASSEA
                     EQLIAKARREIDLSRDKAIEEIRDLAVELSMNATAKLVGKSLDAGDHQSLIDESLKKL
                     GELN"
     gene            5870..6427
                     /gene="atpH"
                     /locus_tag="PKM23_07145"
     CDS             5870..6427
                     /gene="atpH"
                     /locus_tag="PKM23_07145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNY91288.1"
                     /translation="MRTDPLARRYARALFELARDADQTGPVLDELRSFAALLQKEGRI
                     RAYLLSPEVEKKNKQEMVESLLRGQSSPMIRQFIKLLLEKGRQNHLGQIVEAYSKLHD
                     KSIGRVRARVFSAVPLQEVQMEQIRQQVGRYLKSEVLLENKVDAAILGGVIIEFSGVV
                     IDGSLRHQLNQLRQQLRQGKGGAAL"
     gene            6470..7981
                     /gene="atpA"
                     /locus_tag="PKM23_07150"
     CDS             6470..7981
                     /gene="atpA"
                     /locus_tag="PKM23_07150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNY91289.1"
                     /translation="MEIRPEEITSVLKKQIEQFEKQIDVEEVGEVLQVGDGIARVYGL
                     ENVMSMELVEFPNGVYGMALNLEEDNVGCVLFGNDDLIKEGDIAKRTGRVVEVPVGPE
                     LLGRVVNPLGQPMDGKGPINAKNFSPIERKALGVAQRQPVKEPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQIGKTALVVDTFINQKDTDVVCIYVAIGQKASTVAQVVKTLENYGAM
                     EHTIVVASAADSPAPMQYIAPYAGAAMGEYFRDHGQHAAIAYDDLSKHAWAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDSLGGGSLTALPIIETQAGDFSAYIPTNV
                     ISITDGQIYLEPNLFYGGIRPAINAGLSVSRVGGHAQIKAMKKFAGSLRLDLAQYREL
                     EAFAKFGSELDKSTKQQLARGQRMVQLLRQGQYAPLPVEKQVAVIYLGVKGYLDGVAV
                     DRITHAEAEFHRYMEAQHEEVLRSIREQKEVTPEIEKVLTAACEDFIKMFAAE"
     gene            7985..8845
                     /gene="atpG"
                     /locus_tag="PKM23_07155"
     CDS             7985..8845
                     /gene="atpG"
                     /locus_tag="PKM23_07155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNY91290.1"
                     /translation="MATLRTIKRRIASVRSTQQITKAMKMVAAAKLRKAQNQLLSTRP
                     YAHELEEVLGHIAGRMKRDQHPLLEARRIKRICYVVISADRGLCGSFNANISRRALQE
                     VAANKELQPAVVTIGSKGYDYLRRREVPILSNQVDVFNTLDFAQAQRIADLLAGWYTA
                     KKFDQIMVIYNEFKNAVSQKIVVEQLLPIVPLMPEASKFPVGYIFEPNPERILQEICP
                     LNLHVQLWRILLESSASEHGARMTAMGTASDNAADMIKELVLYYNKARQAAITKELTE
                     IIGGAEALKG"
     gene            8894..>9834
                     /gene="atpD"
                     /locus_tag="PKM23_07160"
     CDS             8894..>9834
                     /gene="atpD"
                     /locus_tag="PKM23_07160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNY91291.1"
                     /translation="MSNVGQIAQIIGPVVDVFFEGSALPPINTALEYTRKDGSLLVLE
                     VAQHLGENTARCVAMDSTDGLQRSQEVVNTGAPIKMPVGKATLGRLLNLIGEPIDDLG
                     PLQSEEKWAIHRDAPTFEELETKTEMFETGIKVIDLLEPYAKGGKTGLFGGAGVGKTV
                     LIQELINNIAIQRGGFSVFAGVGERTREGNDLWREFQESGVINMQDLPQSKVAMVFGQ
                     MNEPPGARLRVGLSALTIAEYFREVEGKDVLLFIDNIFRFTQAGSEVSALLGRMPSAV
                     GYQPTLATEMGVLQERITSTKKGSITSVQAIYVPADDL"
BASE COUNT         2028 a         2872 c         2959 g         1975 t
ORIGIN      
        1 atgcggacga agatcccgag ccgatccgcc ttgtcgacga ttttgcggat attctgatag
       61 cactgttccc ggtcgagggt gaggccgagc tgggtcagct tcaccgagat attggcgtcc
      121 agcttgtcgg cggcgatgcg gtcgagcacc ttgaaatagt catcgatggc ttcctggcac
      181 tcctccagct ttctggcgct ttcgccgagc acatccatgg tggcgctgat gcccttgctg
      241 ttcagctcct tgacgacgcg aatgccatct tcgagggtgg gtccggccac atagggcttg
      301 gcgaccatgc ggacaatgaa tttggggatg atgggcagga agaagatgac cagtttgttg
      361 aagagattta tcatgatgca tcctcgcttt gtgggggtca gaaaagagct gcggcggtgg
      421 ttgatggagt ccgggccgac ggggatagtg atgctggaaa aattacatgt ttttgccctc
      481 aatgtcaaga attaactcct gaccgggcgc ccagggggag ggtgtagagg agaaactttt
      541 cattgatttt gaaatttgcg gggagtatat taacacaata tatcagaaaa tggaggaagc
      601 cccccagatg ggcaaaatta taaccatagc gaaccaaaaa gggggcgtgg gcaagaccac
      661 caccgccgtt aacctggcgg cggccctggc tgtggcggag tatccgacgc ttctggtcga
      721 tatggatccc caggccaaca gcaccagcgg ctatggttat gatcagcgcg cgctccaggc
      781 tacaatttac gatgccatta tcaatgagcg ccccgcgcaa tcccttcttt tggacaccca
      841 gctcgattat ctcaagctct tgccctcctc gatcagtctg gtgggcgcgg aggtcgagct
      901 ggtttcggcg atatcacgcg agcagcgcct cgcccgggcg cttgaacctg tgcgcgacca
      961 gttcaagtac attctgatcg attgtccgcc ctccctgggg ctgctgacga tcaactccct
     1021 gaccgcctcc gattccgtcc tcatccccat tcaatgcgaa tactacgccc tggaaggact
     1081 gggtcagctg ctcaacacca tccggctggt gcagaagcac ctcaatgagc acctggcgat
     1141 cgaaggggtg ctgctgacca tgtacgatgc gcgcctcaac ctctcccgcc aggtggcgga
     1201 ggatgtgcgc cggtatttcg agggccgggt ctttgagacc attgtcacgc gcaatgtgcg
     1261 cctcagcgag gcccccagtt tcggcaagcc gatcatcctt tacgacgccg tctgctcggg
     1321 atcggaaaat tatatgagtc tggcagagga aatcatcaag aatggcgaat aaaaaggcgt
     1381 tggggcgcgg cctcagggcg ctcattcctg atatacccgc tgaacaggat gcgccagtcc
     1441 agggcaaggg catcgatttc ctcgatgtgg acaagattgc gcccaatccc ttccagccgc
     1501 gtgaaaaatt tgaccccgaa agcctggccg aactcaagca gtcgattgcg gagaaggggc
     1561 tagtccagcc cgtcactgtc cgcaagcacg gcgcagggta ccagctcatc tccggcgaac
     1621 ggcgtctgcg cgcagtgcgc gaccttggcc ttcagaagat tcccgcctac atcctcgagg
     1681 tcgagaccga tgaggaaatg ctcgaactcg cgatcatcga gaatatccac cgcgaggatc
     1741 tgaacgcgat cgatatcgcc catggctaca agcggctcat tgatgagtgc aagctcaccc
     1801 aggaagaggt tgcggtcaag gtcggcaagg accgcaccag tgtcgccaac ttcctccgat
     1861 tgctcaaact gccgcgacgc atccaggaga gcgtccagag cagcgaaatc tccttcggcc
     1921 acgcccgcgc cctgctctcg ctggccagca gtgaggatca gctcgagatg tggaagaaga
     1981 tagtcggcgg cagcctctcc gtgcgcaagg tcgaggagat ggtgcgggag ccggagaagg
     2041 gcaaggccaa gccggccaag gtgacggcag cgccgaatta ttacatggaa gagctggaaa
     2101 acaagctgcg tcttcacttg gccaccaagg tggagttgaa gagcagaaac cggggcggcg
     2161 ctatcgagat cgaatactac tcctcagatg aacttgagcg catcatcgcc ctcattctcg
     2221 gcgagtgata tgttcggcag gctggcaaaa atgttcggca gaaagaaggc ggagggcact
     2281 gcgcgcagcc accgcagatc actgcagatt ctggtgatcc cggacgatca ggcggagccg
     2341 cgccattatc agatctcgat gcgcaagttc aggatcctgc gcgttgctgc ggtggtgctg
     2401 gcccttcatc tttttttagg gatcgtggcc tatctgcagt gggcgcgcct ggcggtccga
     2461 tatgatgacc tgagcaagat caacggccag ctggtcgaga acagcaaaaa agtgtatgag
     2521 ctggcagccg tgtttcaaaa gatcgaggca accgatgcca agctgcgcag cgccctgggt
     2581 ttggatgaga accgggggag cgctaccctc gcttcggatg aggtctttga gcctccccgg
     2641 cgcgccctcc ccgtcgagcc catcctcgag ccgcgccggg agcgccccaa accggtagag
     2701 gtcaatctcg gcgaaaagct cggatttctc cagcagtcca gggagatcgg cattcacgaa
     2761 tatctcagga gcctgcccac ctatctgccc gtggccgggg ttctgacgcg caactactcc
     2821 gatgaggggg ccgcccacac tggcatcgat atcgctgccg cgaggggctc tttcatcagc
     2881 gccgccgggg acggggtggt ggtcttttcg ggctggaccc aggatctggg caatatggtg
     2941 atcatttacc atggcgacgg gttcttcacc tattacggcc acaaccagcg caatctggcg
     3001 agtcgttcca ccctggtgcg caagggagac cacattgccc tgctcgggtc ctcgggcgag
     3061 agcacggcgc cgcatctgca ttttgaatta tggaaagacg ggatcgccct caatcctgcg
     3121 gactatattc ttgcgttcgc acggatgtga gcgggcggcc cgagcgatgc accttaatgg
     3181 aaagcgacca tatgaaaagc acggataaac cgggggagtt gggcaccatt ctcggcaagg
     3241 gcgcagtcgt tgacggcaac atcaaggtcg aacacagcct gcgcgtggac ggcaaggtgg
     3301 ttggggatgt cacctccggg gacaccctca tcgtcggcgc cgagggggaa atcaagggca
     3361 atgtcaaggt gaagaacctt gtcctcggcg gccggatcaa cggatccgta ctctctcccg
     3421 ggcggaccgt tctcgaggcc cgttccgagc tgcgcggcga gctcaagacc ggcaagctgg
     3481 tcatcgatga gggcgccata tttgacggca aatgctcgat gtcagacgcc aaagcggaga
     3541 ccggcaggac tccgggcgag gcggacggag agacgatcat caaatagaaa agcggggcgc
     3601 agtgacagaa gagggcggca gaaacagacc ggcggtcggg ccctatatga atctgggcat
     3661 gcagctggcc gtcgccgtga tcctcggcac ggccctgggc tactggctgg atagcaaact
     3721 gggcacctcc ccgctcttcc tcctgctcgg agtaatagcg gggggaacgg cggggttcat
     3781 gaatgtctac aagaccgtct accccgaccg ccgggataaa gaatcgagca caaagcagag
     3841 atgaaatcac ccatgcggat ctggcgtctg gttggctgga cggttccggc cttcctggtg
     3901 ctgctgatcc ctcttcatta ctacggccgg cccggcatga ccagctccta caccctcggc
     3961 tatctggtca gcctttttaa tatcgtcttc agcctggtat cgatccagtg ggcttttcgc
     4021 cgcaaggtca agacctttta cgcagtggtg ctggggggca tggccctgcg cttcatcctc
     4081 ctcgcggtca tggtctacct ggtgatcgcc gtcctgcact ggcctctggc cggatttctg
     4141 gtctcttttg ttctcttttt tcttgtgctg cagtattttg aaatcagata tatcaattcg
     4201 gaactgaaag gcagatcttc cgcgaatcat gatcacacag atagttgaac acgcgtccga
     4261 gcacggcgag gctgtgcagc aggcggccga acacgcggct gagcatgggg agagcggcgt
     4321 cggctttatc atgcaccata tcatggccca tccggtgatc aagctgccga cggtttacgg
     4381 gattgacctc tccgtcacca atcatgtgct ctggatgtgg atcgtctcgg ctctcctgat
     4441 cctgggtttt tatgtggctt tccgcaaacc gaagctggtg ccgcacggac tggcaaacgc
     4501 cctcgagtcc ctggtggttt tcatccgcga cgacgtcatc atgcccaatc tcggtcacga
     4561 ggcccgcgcc tacgccccct atctgctgac ggcttttttc ttcatcatgc tctgcaacct
     4621 cgccgggctg gtgccctacg gcgccaccgc caccggcaac atcggggtga cggcgaccct
     4681 cgccctcttc accttttttg tggtgcagat tgccggggtg cgcaagcacg ggttcctggg
     4741 ctatctctcg accttcagcc cgccgggaat cccctttttg ctcaagttca tcatgatccc
     4801 ggtcgaggtg atcagcatga tcaccaagca cttcgccctg gcgattcgtc ttttcgccaa
     4861 catgatcgcc ggccatatca ccatcctggc cctgttgagt gtcatctttt tattcaagaa
     4921 ctggctggtc tcgcccttcc ccctggcgat catccttttt tccgccattc tggaggtgct
     4981 gatctccctg atccaggcct atgtctttac gattctctcg gctgtattca tcagcgcctc
     5041 cgtggctgag gggcactgag atcggtgtac agataacaac cttgcgtcat tcactacaaa
     5101 ctaacatttc ttgtatcagg aggtagtatt atgaatccca ctgcaattgc ctatctggct
     5161 gccggatttg gatctgctgc ggtgaccttt tctgctgcgt ttggcatcag caaactggcc
     5221 agtgcggcca tcagcggcat tgcgcgtcag cccgaagccg ccggcgacat tcgcggcgcc
     5281 atgatcatca ccgcggccat gatcgaaggc gtggccctgc tctctgtggt tatctgtctg
     5341 ctgctcggct tcaaataaac ctgagggcgg ttacacctat ggaactgatg acacccgcgg
     5401 gtgggaccat attctggacc accctgacct ttcttctcct cttgtttgcg ctgaccaagg
     5461 tgggctggaa gcccatcctg agcatgctgg acgagcgtga gaagaagctg aaagagtctt
     5521 tggagctggc cgagcaggcg aagatcgcct cgcagaagac cctcgcagag caaaatcagc
     5581 tgctcgagac cgcccgcagg gaggcccagg agattctggc ccgcaaccgc aaggcggctg
     5641 aggcgaccaa agaggagatc atcaaaaagg cgagctcgga ggctgaacag ctcatcgcca
     5701 aggcgcgccg ggagatcgat ctcagccgcg acaaggccat cgaagagatc cgcgatctgg
     5761 ccgtggagct gtcgatgaat gcgacggcca agcttgtcgg caaatccctc gacgccggcg
     5821 atcaccagtc gttgatcgat gaatccctga agaaactggg tgagctgaat tgagaaccga
     5881 tcccttagcc agacgctatg cgcgcgccct tttcgagctg gcgcgcgatg cggaccagac
     5941 cgggccggtg ctggatgagc tgcgctcttt cgccgccctc ttgcagaagg aggggcgcat
     6001 ccgggcctac ctgctctcac cggaggtgga gaaaaaaaac aagcaggaga tggtggaaag
     6061 cctcctgcgc gggcaatctt cacccatgat ccggcagttc atcaagctcc tgctcgaaaa
     6121 ggggcggcag aaccacctcg gccagatcgt ggaggcttac agcaagctcc acgacaaaag
     6181 tatcggccgt gtgcgcgccc gggtcttttc cgccgtgcct ctgcaggagg tgcagatgga
     6241 gcagatccgg cagcaggtcg ggcgctatct caaatctgag gttctgctcg agaacaaggt
     6301 cgatgccgcc atcctcggcg gcgtcatcat cgagttttcc ggagtggtga tcgacggcag
     6361 cttgcggcac cagctcaatc aattgcgcca gcagctgcgc cagggcaaag gcggcgcggc
     6421 gctttagtgc agcatactca tttcatattt catatagcag gttacaacca tggaaatcag
     6481 acccgaagag attacctcgg ttcttaaaaa gcagatcgaa caattcgaga agcagatcga
     6541 cgtcgaggag gtgggtgagg ttctgcaggt tggcgacggc atcgcccgcg tctatgggct
     6601 tgagaatgtc atgtccatgg agctggtcga gtttcccaac ggcgtctatg gcatggccct
     6661 gaaccttgaa gaggacaatg tcggctgcgt gcttttcggc aacgacgatc tcatcaagga
     6721 gggggatatt gccaagcgca ccggccgggt ggtggaagtg ccggtcgggc cggagctgct
     6781 gggccgggtg gtcaaccccc tcggccagcc gatggacggc aaggggccga tcaacgccaa
     6841 gaatttcagc cccatcgaac gcaaggccct gggtgtggcc cagcgccaac cggtgaagga
     6901 gccgctgcag acgggcatca aggcgatcga ctcgatgatc cccatcggcc gcggccagcg
     6961 cgagctgatc atcggcgacc gccagatcgg caagaccgcc ctggtagtgg acacctttat
     7021 caaccagaag gataccgacg tcgtctgcat ctatgttgcc atcggccaga aggcctcgac
     7081 cgtggcgcag gtggtcaaga ccctcgagaa ttacggcgcc atggagcaca cgattgtcgt
     7141 cgcttccgcc gccgattccc cggccccgat gcagtacatc gccccctatg cgggcgcggc
     7201 gatgggcgag tatttccgcg accatggcca gcatgcggcg atcgcctacg atgatctctc
     7261 caagcacgcc tgggcctatc gtcagctctc cctgctgctg cgccggcccc ccggccgcga
     7321 agcctatccg ggcgacgtct tttatcttca ttcccgtctg ctcgagcgcg cggccaagct
     7381 gagcgacagc ctgggcggag gttcgctgac agccctgccg atcatcgaaa cccaggcggg
     7441 cgatttttcc gcctatatcc cgaccaacgt catctcgatc accgacggcc agatctacct
     7501 ggagcccaac ctcttctatg gcggcatccg tccggccatc aacgccggcc tttcggtttc
     7561 gcgcgtcggc ggccatgccc agatcaaggc gatgaagaaa ttcgccggca gtctgcggct
     7621 cgatctggcg cagtaccgcg agctcgaggc ctttgccaag ttcggttccg aactcgacaa
     7681 atcgaccaag cagcagctgg cgcgcggcca gcgcatggtc cagcttttgc gccagggcca
     7741 gtacgccccg ctgccggttg agaagcaggt ggcggtcatc tatctcggcg tcaagggtta
     7801 tctcgacgga gtcgcggtcg accgcattac ccatgcggag gccgagtttc accgctacat
     7861 ggaggcccag cacgaggagg ttctgcgcag catccgtgag caaaaagagg tgacaccgga
     7921 gatcgaaaaa gtgctgacgg cggcctgcga ggatttcatc aagatgttcg cggcggaata
     7981 agcgatggcc acattaagaa cgatcaagcg ccgcatcgcc agtgtgcgca gtacacagca
     8041 gatcaccaag gcgatgaaga tggtggcggc ggccaagctg cgcaaggccc agaaccagct
     8101 gctcagcact cgcccctatg cgcatgagct ggaggaggtg ctgggccaca tcgccggccg
     8161 gatgaagcga gaccagcatc cccttcttga ggcgcgccgg attaaaagga tctgttatgt
     8221 tgtgatctcg gctgatcgcg gcctctgcgg cagcttcaat gccaacatca gccgccgggc
     8281 tctccaggag gtcgcggcca acaaggaact gcagccggcg gtggtcacta tcggcagcaa
     8341 ggggtatgat tacctgcggc gccgtgaggt gcccattctg tccaatcagg tggatgtttt
     8401 caataccctt gatttcgccc aggcgcagcg tattgccgac ctactggccg gatggtatac
     8461 cgccaaaaaa ttcgaccaga tcatggtcat ctacaacgag ttcaagaatg cggtttccca
     8521 gaaaatcgtg gtcgagcagc tgctgccgat cgtgccgctg atgcccgagg cgagcaaatt
     8581 tccggtgggc tacatcttcg agcccaatcc ggagcggata ctgcaggaga tttgtcccct
     8641 caatctgcac gtgcagctct ggcgcatcct gcttgaatca agcgcctcgg aacacggcgc
     8701 gcggatgacg gccatgggca cggcctcgga taatgccgcc gacatgatca aggagctggt
     8761 gctctattac aacaaggcgc gtcaggcggc tatcaccaag gagctgaccg agatcatcgg
     8821 tggtgctgag gcgctcaagg gatagccgga ttttgaagag tgagaacctt atcgattgtc
     8881 aggagagtat gggatgtcaa acgttggtca aatagcgcag atcatcggtc cggtcgttga
     8941 tgtctttttc gagggcagtg cgctgccacc catcaacacc gccctggaat atacccgcaa
     9001 ggatggatcc cttctggtgc tggaggtggc gcagcatctg ggtgagaaca ccgcccgctg
     9061 tgtcgccatg gattcaacgg atggattgca gcgcagccag gaggtggtga acaccggtgc
     9121 gccgatcaag atgccggtgg gcaaggcgac cctgggacga ttgctgaatc tcatcggcga
     9181 gccgatcgat gatctggggc cgttgcagtc cgaagagaag tgggccatcc accgcgatgc
     9241 ccccaccttc gaggaattgg agaccaagac cgagatgttc gagaccggca tcaaggtcat
     9301 cgacctgctc gagccctacg ccaagggcgg caagaccggt cttttcggcg gtgccggtgt
     9361 cggcaagacg gtgctgatcc aggagctgat caacaacatt gccatccagc gtggcggttt
     9421 ttcggtcttt gcgggggtgg gcgagcgcac ccgtgagggc aatgacctct ggcgtgaatt
     9481 ccaggagtcg ggcgtcatca acatgcagga tctgccccag tccaaggtgg cgatggtttt
     9541 cggccagatg aacgagccgc ccggcgcccg tctgcgcgtc ggtctttcgg cgctgaccat
     9601 cgctgaatac ttccgtgagg tggagggcaa ggatgtcctc ctcttcatcg acaatatttt
     9661 ccgcttcacc caggccggtt ccgaggtctc agccctgctc ggccgcatgc cctcggcggt
     9721 gggctatcag cccactctgg ccacggaaat gggtgtgctg caggagcgca tcacctcaac
     9781 caaaaagggt tcgatcacct cggtgcaggc catctacgtg cccgccgacg acct
//
LOCUS       DAOPWU010000107         9812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_6969_length_9812_cov_4.056062, whole genome shotgun sequence.
ACCESSION   DAOPWU010000107 DAOPWU010000000
VERSION     DAOPWU010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6969_length_9812_cov_4.056062"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1277
                     /locus_tag="PKM23_07165"
     CDS             102..1277
                     /locus_tag="PKM23_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNY91292.1"
                     /translation="MASVPIGKTAIETLKNIPIAIKETRPVFLLSVPSLAQNFRKNIE
                     KGIRQKGAKVEALFNKALKAAYAYNGLGFDRGKGWRAVYKPLLLLYDKLLFKKVRENF
                     GGRLEFFIGGGALLDIELQRFFYAIGIPMFQGYGLSEAAPIISANVPRRHKLGSSGTI
                     VANLEVRICDEKGNDLPRGEKGEIVVRGENVMAGYWKNEKATSEALREGWLYTGDLGY
                     LDKDGFLYVLGRFKSLLISNDGEKYSPEGIEEAVCSASRLIDQIMLYNNQSPYTVALV
                     VPNKEALLRHLKANNLAPQSELGQREALKAIEQEIARYREGGEEAGLFPGKWLPAAVG
                     VLGEGFTEQNHLLNSTLKMVRGKIIEAYKPRLDYLFTPEAKDICNKQNMTIISRLEE"
     gene            complement(1367..2194)
                     /locus_tag="PKM23_07170"
     CDS             complement(1367..2194)
                     /locus_tag="PKM23_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNY91293.1"
                     /translation="MSNGAINRPESGWRSRLHEIIFESDTRAGRIFNIILSLAIVLSV
                     IAVLLDSVHSIRDRFGTLLFIIEWFFTILFTVEYILRLLSLRQPRRYAVSFFGIVDLL
                     AIVPTYFSLIFPGAMYFLSFRILRLLRIFRVLKLSEYLRDAQIIVNALLASRRKIGVF
                     LLTVLLLSVIMGSLIYVVEGEQNGYTSIPTSIYWAIVTMTTVGYGDLAPQTPLGKMLA
                     SVIMILGYAIIAVPTGIVTVEMSRSQIRRVSTQACPDCGAEGHDPDARFCKYCGGVL"
     gene            complement(2765..3943)
                     /locus_tag="PKM23_07175"
     CDS             complement(2765..3943)
                     /locus_tag="PKM23_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91294.1"
                     /translation="MARVWKGMAGGLLLLAAAGVAQEECTVAVVSGSATSDGRPLLWK
                     NRDSSSRENSVCFFTGPRYDFVGVINSGDTTQVWMGLNTAGFALMNSESMDQDGDSAD
                     TEGYFMKQALGECARVEDLEALLRRTALTGRGTRANFGCIDAFGGAAFFEAGNFRHRK
                     FTTQDSGLAPAGFLVRANFSMTGRGEEAYGGWRYHRARTLLSSRAAAGNLDLTYMLGS
                     VVRDLVSDDLNPYPLPFRGRSGEAPEGFIHAQNTINRHRTVSAVLFQGVKPGEHPALS
                     TMWVLLGEPVAAVALPLWPASGEVPPAFGGAGKSGLNDAFSRLRARLYHDRDWPQHLD
                     TGRLVSGRRPWLRERSALEGEVLQETAEAMARWRRQRPAGSEMADLQKKLLRKVIRAL
                     "
     gene            complement(3946..4740)
                     /gene="cdaA"
                     /locus_tag="PKM23_07180"
     CDS             complement(3946..4740)
                     /gene="cdaA"
                     /locus_tag="PKM23_07180"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNY91295.1"
                     /translation="MSLTLFKIGFIPVTVMDIADILIMSFILYRSYLFIRGSRGAQML
                     VGLVIILLFSIITPVLQMRGISWIFQNLRTVWLVAFVILFQPELRRILLSVGQSRLIR
                     YFVKVSGNRVIEEVVEAAAQLQQRGYGALIVMGRETGLKPVAETGVRIQAEVSAPLLV
                     SLFNPRSPLHDGAVIIQNDLIDTAKCILPLTENPTPQLNLGTRHRAAIGLSEESDAVV
                     IVVSEETGRISVAMAGELKYDLTLENLRHILYQTFNQALPKGEKGA"
     gene            complement(4813..5667)
                     /gene="folP"
                     /locus_tag="PKM23_07185"
     CDS             complement(4813..5667)
                     /gene="folP"
                     /locus_tag="PKM23_07185"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNY91296.1"
                     /translation="MTPGIWRCGGRRIEWGRRTLIMGILNITPDSFSDGGRYFSTTAA
                     LAEARLLIDEGADILDVGGESTRPGSEPVSAAEEMERVLPVIAALRQNFDCPLSVDTW
                     KADVAEAALAAGADIINDISGLSFDPRMAGVIARSGAGVVIMHIRGTPRDMQKDPHYE
                     DVTGEVGEWLARQVELALAAGIRREQIVIDPGLGFGKRLEDNFTLLRELGRLGELRLP
                     ILAGPSRKSFIGRVLDLPPDDRVEGTAAAVTAAILNGARIVRVHDVRAIRRAALIADA
                     IRSGPEGF"
     gene            complement(5682..7649)
                     /gene="ftsH"
                     /locus_tag="PKM23_07190"
     CDS             complement(5682..7649)
                     /gene="ftsH"
                     /locus_tag="PKM23_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNY91297.1"
                     /translation="MHRQDGGGDSQPPQRQGGPPRRPAGPQRSQPEGPQWGRMLRPLL
                     IWVGLIFLALAISQQFARQEPQESVLTYDGFKNYLQMGKVESIKVEDDVIHGQLKPDA
                     ALEGQRGFQVILPPRASLETFDEWIQKYDVEVIFKKSGPSIWSSLIYILPWILFIGIW
                     IFFMRRMQGANNKGIFAFGKSRARMWTDNMPHVTFADVAGADEAKQELQEIIEYLREP
                     EKFQRLGGKIPKGALLLGPPGTGKTLLAKAVAGEAGVPFFSMSGADFVEMFVGVGASR
                     VRDLFDQGKRNAPCIIFIDELDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFDSNEG
                     VILIAATNRPDVLDSALLRPGRFDRQIVVDRPDVRGREGILKVHTKKIPLAKAVDLAI
                     LAKGTPGFSGADLANMVNEAALLAARKDKTQVEAVDFEEAKDKVMMGIERKSLLISEE
                     EKKSTAYHEAGHVLVARLTPGSDPVHKVSIIPRGRALGVTTMLPMDEKHNYSRTYCLA
                     IMRQLLGGRAAEQLILNELTTGAGDDIKRVTELARKMVCEWGMSEKLGPLTFGEKQEE
                     IFLGREIAQHRDYSEATAHLIDTEIRSLVESAAADVARLVSENVELLHAIANALLERE
                     MLDGEEIDKIMNGIALPKRKRTAKKTPAKKG"
     gene            complement(7697..8248)
                     /gene="hpt"
                     /locus_tag="PKM23_07195"
     CDS             complement(7697..8248)
                     /gene="hpt"
                     /locus_tag="PKM23_07195"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNY91298.1"
                     /translation="MTQRSEFEKVVYDGGFRTLLTAEAIDARIREVGRQITEDYRGLQ
                     PIMIGVLNGAFLFMADLIRHVEIDLEVDFIKISSYGNAKVSSGIVRMLKDINADVKGR
                     HILLVEDIVDTGGSIKFLHEKFMALEPASLRFVTLLIKPANVETDIPIDYVGFSIANE
                     FVVGYGLDYKQILRNLPAVYVMD"
     gene            complement(8232..9686)
                     /gene="tilS"
                     /locus_tag="PKM23_07200"
     CDS             complement(8232..9686)
                     /gene="tilS"
                     /locus_tag="PKM23_07200"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNY91299.1"
                     /translation="MFDRFIAYIREHNLLQPGMRVLVALSGGLDSVVLLDLLAQLAPE
                     WRLALTAAHFNHQLRGAEADRDEDFVRTLCAGRALPCEVGRGDVRVYARAHHLSLEAA
                     ARALRYDFLIASARRCGCAAIATAHHAGDQAETVLDRLIRGAGVRGLAGIVPRSAIAA
                     APDLPCTAASPGAGPPAPLLLIRPLLFASRAEIAAHAAARGLEYCTDTTNADRRIRRN
                     RIRHELLPLLEEYNPAIEAVLGRTAGHMREVDSYMRSQARAALPSCTVVAKSGKIILE
                     KEAFLSYFTLLQKYILREAWEGIGGDGRDLDEAFWSGWEQFLAAGRTDRGLAAGSGEF
                     WQSETRLVLLAKRPTAAAMAIPAAPGRYPLREGLTLEINRVSPPLELIEKNSDHRIAW
                     IDADLVQSTLELRPPRPGDRMQPLGLKGSKKVADLLAGAGVPVYERGGIYLLCSGGEI
                     VWICGLRAAGPFRVTPRTTNIFQLKVEIDRDSAL"
BASE COUNT         1856 a         3117 c         3094 g         1745 t
ORIGIN      
        1 gctgccgatt ccggaatggt acctctccct gctcatcctg ccctgggacc acagctttgc
       61 ccataccgcc ggcatctaca ccctgatgaa aaacggccag catggcctcc gtcccgatcg
      121 gcaaaaccgc catcgaaacc ctgaaaaata tcccgatcgc catcaaggag acccggccgg
      181 tttttctgct cagcgtcccc tccctggcgc agaatttccg caaaaacatc gagaagggca
      241 tccgccagaa gggggccaaa gtggaagccc tcttcaacaa ggccctcaag gcggcctacg
      301 cctacaatgg cctcggcttt gaccgcggca agggctggcg cgccgtctac aaacccctgc
      361 tgctgctcta cgacaagctg ctcttcaaaa aggtgcgcga aaatttcggc gggcgcctcg
      421 aattcttcat cggcggcggc gccctcctcg atatcgagct gcagcgcttc ttctatgcca
      481 tcggtatccc gatgttccag ggctacggac tgagcgaagc cgctccgatt atctccgcca
      541 acgtgccgcg ccggcacaaa ctcggctcat ccggcaccat cgtcgccaat ctcgaagtca
      601 ggatctgcga cgaaaagggc aacgatctgc cccggggcga aaagggggag atcgtggtgc
      661 ggggtgaaaa tgtcatggct ggctactgga agaacgaaaa ggcaaccagc gaggccctgc
      721 gcgagggctg gctctacacc ggcgacctcg gctacctcga caaggacggt tttctctatg
      781 tgctcgggcg cttcaagagc ctgctgatct ccaacgacgg cgaaaagtac agcccggagg
      841 ggatcgagga ggcggtctgc tccgcctccc ggctgatcga ccagatcatg ctctacaaca
      901 accagtcgcc ctacaccgtc gccctggtcg tgcccaacaa ggaggccctg ctgcgccatc
      961 tcaaggcgaa taatcttgcg ccccagagcg aactgggaca gcgcgaagcc ctcaaggcca
     1021 tcgagcagga gatcgcccgc tacagggagg ggggcgaaga ggccggcctc ttccccggta
     1081 aatggctgcc cgcagcggtc ggcgtgctgg gcgagggctt taccgaacag aaccacctcc
     1141 tcaacagcac cctcaagatg gtgcgcggca agatcatcga ggcctacaaa ccccggctcg
     1201 attacctctt cacccccgaa gccaaggata tctgcaacaa acagaacatg accatcatct
     1261 cgcggctcga ggagtaggaa cagcattcct ctcaccccga tggccatata atgaaaaagg
     1321 cggggccccg aatgggctcc gccttttttt tctcctcacg cgaggcctac agcacccccc
     1381 cgcagtattt gcagaatctg gcgtcgggat cgtgcccctc ggcgccgcag tcgggacagg
     1441 cctgggtgga aaccctcctg atctggctgc gcgacatctc aacggtgacg atgccggttg
     1501 ggaccgcaat gatggcgtag ccgaggatca tgatcaccga ggcgagcatt ttgccgagcg
     1561 gggtctgcgg cgccagatcg ccatagccca ccgtggtcat ggtgacaatc gcccagtaga
     1621 tgctggtcgg gatgctggta tagccgttct gctcgccctc gaccacatag atcagcgatc
     1681 ccataatcac cgaaagcagc agcacggtga gcagaaagac cccgatcttg cggcggctgg
     1741 cgaggagggc attgacgatg atttgggcgt cgcgcaggta ttcgctcagt ttgagcacac
     1801 gaaagatgcg cagcagacgc agaatacgga aagagagaaa gtacattgcc cccggaaaaa
     1861 tcaggctgaa ataggtgggc acgatcgcca gcagatcgac aatcccgaaa aaactgacag
     1921 cgtagcgtcg gggctgccgg aggctgagca gacgcaagat atactcgacg gtgaagagga
     1981 tggtgaaaaa ccattcaatg atgaaaagga gggtgccgaa gcggtcgcgg atggagtgca
     2041 cgctgtcgag caggaccgcg ataacgctga ggacgatggc aagcgaaaga atgatattga
     2101 agattctgcc ggcccgcgta tcggactcga agatgatttc atgcagcctg ctgcgccaac
     2161 cgctttcagg acggttaatg gccccgtttg acatggccgt ttcctttgcc cgactttttt
     2221 ggtataaata tagccaatat atcggcctgg cgcaagcaat tcgtcagagc aaaaaaaagg
     2281 gcagcgctgt gctcggacgc tgcccttctg gctgtggtga gtcataagag aggagggatt
     2341 ccatgctgaa aacggcactc ccggagaaaa tcgcgcccgc accggcacat ccacctcttc
     2401 ttcacaccgg tccagtgcgc tgctcgtacc ctactccacc tctggtcctg atcgtgtgcc
     2461 gtgacgggcc gcccccctaa ggttgcgtcc cgaattaacg gcctgacccg tccacccgaa
     2521 ccccgtcatc catgacacgg acagaaccgc tgctttgcaa tgctgctcca ctgttcaatg
     2581 ttgccccgct tgtctccagg agccgctttc attctgctct tttgcaaaac tcatgccaga
     2641 ttccggaagg gagagcgggt gagttttgtg cctgcgaagg ggtttgcctg cggcggcggc
     2701 cggggcggat tgttccaggg tgatacgggg aacgggggaa tgactcagcg gaggtcagtg
     2761 cactttaaag agcgcggatc accttccgca gcagcttttt ctgcaaatcg gccatctcgg
     2821 agccggccgg acgctggcgg cgccatcgcg ccatcgcctc cgctgtctcc tgcaacacct
     2881 cgccctcgag ggcagagcgc tcccgcagcc atggccggcg gccggaaacc agccgcccgg
     2941 tgtccagatg ctggggccaa tcgcggtcat gatagagccg ggcgcggagg cggctgaagg
     3001 cgtcattgag tccggatttt ccggcgccgc caaaggccgg cggcacctcc ccgctggccg
     3061 gccacagggg cagggctacg gccgccaccg gctcgccaag gagaacccac atcgtcgaga
     3121 gggcaggatg ctcacccggt ttgaccccct gaaaaaggac agccgagacg gtgcggtggc
     3181 gattgatggt gttctgggca tggataaagc cctcgggagc ctccccggac cggccgcgga
     3241 agggcagcgg atagggattg aggtcgtcgc tgaccaggtc gcgcacgacg cttccgagca
     3301 tgtaggtgag gtcgaggttc ccggccgccg cccggctgct cagcagggtg cgggcgcgat
     3361 ggtagcgcca gccgccatag gcctcctctc cccggccggt catggaaaaa ttggcccgca
     3421 ccagaaagcc ggccggcgcc agccccgagt cctgggtcgt aaatttgcga tggcggaaat
     3481 tgcccgcctc gaaaaaggcg gcaccgccaa aggcgtcgat gcagccgaaa ttggcgcgcg
     3541 tgccgcggcc cgtcagggcg gtccggcgca gcaaggcctc aagatcctcg acccgtgcac
     3601 attcgcccag ggcctgcttc ataaaataac cctcggtatc ggcgctgtcc ccgtcctggt
     3661 ccatcgattc gctgttcatc aacgcaaagc cggccgtgtt cagccccatc cagacctggg
     3721 tggtgtcgcc gctgttgatc accccgacaa aatcatagcg tggacctgtg aaaaagcaga
     3781 ccgaattctc gcggctgctg ctgtcgcggt tcttccagag caggggccgg ccgtcgctgg
     3841 tggcgctgcc gctcaccacc gccaccgtgc actcctcctg cgccacaccc gccgccgcca
     3901 ggagcagcag accgcccgcc atccctttcc atacacgcgc catcgtcatg ctcccttctc
     3961 ccctttcggc agggcctggt tgaacgtctg atagaggata tggcgcaggt tctcgagggt
     4021 gagatcatac ttcagctccc cggccatcgc cacggagatc cgcccggtct cctcggagac
     4081 gacgatcacc accgcatcgc tctcctcgga gaggccgatg gcggcgcggt ggcgggtgcc
     4141 gagattgagc tggggcgtcg gattctcggt caggggcagg atgcacttgg cggtgtcgat
     4201 gaggtcgttc tggatgatca ccgcaccgtc gtgcagcggc gagcgcggat tgaagagcga
     4261 gaccagcagg ggagccgaga cctccgcctg gatgcgcacc ccggtctcgg cgaccggttt
     4321 gaggccggtc tcgcggccca tcacgatcag ggccccatag ccgcgctgct gcagttgggc
     4381 ggccgcttca accacctcct cgatcacccg gttgccgctg accttgacga aatagcggat
     4441 gagccggctc tggccgaccg aaagcaggat gcggcgcagc tcaggctgaa aaaggatgac
     4501 aaaggccacc agccagactg tgcgcaggtt ctggaagatc cagctgatgc cgcgcatctg
     4561 cagcaccggg gtgatgatcg aaaaaaggag gatgatgacc aggcccacca gcatctgggc
     4621 gccgcgcgag ccgcgtatga agagatagct gcggtagagg atgaaggaca tgataaggat
     4681 gtcggcgatg tccatcaccg tgacggggat gaaacctatt ttaaagaggg tcagggacat
     4741 gctcacaggc tcaacaggat ttccagagta caggggtgag gcccggcgtg gccaggatgc
     4801 tgcagttgcg gtttaaaagc cctccggccc cgagcgaatg gcatcggcga tgagagcggc
     4861 gcggcggatg gccctgacgt cgtggacgcg gacgatgcgg gcgccgttga gaatggccgc
     4921 agtgaccgcc gcggccgtcc cctccacccg atcgtccggg ggcagatcca gaacccggcc
     4981 gatgaaggat ttgcgcgagg ggccggcgag aatgggcagc ctcagctccc caagacgccc
     5041 gagctcgcgc aatagggtga aattgtcctc gagccgcttg ccgaagccga ggccgggatc
     5101 gatcacgatc tgctcgcgcc gtatccccgc cgccagggcc agttcgacct ggcgcgccag
     5161 ccactccccg acctcgccgg tcacatcctc ataatgggga tccttctgca tgtcgcgcgg
     5221 ggtgccgcgg atgtgcatga tcaccacccc ggcgccgctg cgggcgatca ccccggccat
     5281 gcgcgggtca aaactcaacc cgctgatgtc gttgatgatg tcggctccgg ccgcgagcgc
     5341 cgcctcggca acatcggcct tccaggtatc gaccgaaagc gggcagtcga aattctgccg
     5401 cagcgccgcg atcaccggca gcacccgctc catctcttcc gctgcagaga ccggttcgga
     5461 gccggggcgg gtggactcgc ccccgacatc gaggatgtcc gccccctcgt cgatcaggag
     5521 cctggcctcc gccagggcgg ccgtggtgga aaaataacgc ccgccatcgc tgaacgagtc
     5581 gggcgtgata ttaagaatgc ccatgatcag ggtccgccgc ccccattcga tgcgacggcc
     5641 gccgcagcgc cagatcccgg gcgtcaccac caactcccgc ctcagccttt tttggccggg
     5701 gtctttttcg cggtgcgctt gcgtttgggc aaggcgatcc cgttcatgat cttgtcgatc
     5761 tcctcgccgt ccagcatctc gcgctccagc agggcattgg cgatggcgtg aaggagttcg
     5821 acattctcgc tgaccagccg ggcgacgtcg gccgcggcgc tctccaccag gctgcggatc
     5881 tcggtgtcga tgagatgggc agtcgcctcg ctgtagtcgc ggtgctgggc gatctcgcgc
     5941 cccaaaaaga tctcctcctg cttttcgccg aaggtgaggg gaccgagctt ttcgctcatg
     6001 ccccattcgc aaaccatctt gcgcgccagc tcggtgaccc gcttgatgtc gtcgccagca
     6061 ccggtggtca gctcgttgag gatgagctgc tcggcggcgc gtccaccgag cagctgacgc
     6121 atgattgcca ggcagtaggt gcggctgtaa ttgtgcttct cgtccatcgg cagcatggtg
     6181 gtgacgccca gggctcgccc gcgcgggatg atgctcacct tgtgcacggg atcggagccc
     6241 ggggtgagcc gggcgaccag cacatgcccg gcctcatgat aggccgtgct tttcttctcc
     6301 tcctcactga tgagcaggct cttgcgctcg atgcccatca tgaccttatc cttggcctct
     6361 tcgaaatcga cggcctcgac ctgggttttg tccttgcgcg ccgccagcag ggccgcctca
     6421 ttgaccatgt tggccagatc ggcgccgctg aaaccgggcg tccccttggc cagaatggcc
     6481 aggtcgaccg ccttggcgag cgggatcttt ttggtgtgga ccttgaggat gccctcgcgg
     6541 ccgcgtacat cggggcggtc gaccacaatc tggcggtcga aacggccggg ccggagcagg
     6601 gccgaatcga ggacatcagg ccggttggtc gcggcgatga ggatcacacc ctcgttgctg
     6661 tcaaagccgt ccatctcgac caggagctgg ttgagggtct gctcgcgttc gtcgtgaccc
     6721 ccgccgaggc cggcgccgcg gtggcggcca acggcatcga gctcgtcgat gaagatgatg
     6781 cagggggcat tgcgcttgcc ctggtcaaag agatcgcgca cgcgcgaggc cccgacgccg
     6841 acaaacatct cgacaaagtc cgccccgctc atgctgaaaa agggcacccc cgcctcaccg
     6901 gccacagcct tggcgagcag ggtcttgccc gtgcccggag gaccgagcag cagcgccccc
     6961 ttggggatct tgcccccgag gcgctggaat ttctccggtt cgcgcaggta ctcaatgatc
     7021 tcctgcagct cctgcttggc ctcgtccgca cccgccacat cggcaaaggt gacgtggggc
     7081 atattatcgg tccacatccg cgcccgcgac ttgccgaagg cgaagatgcc cttgttgttg
     7141 gccccctgca tacggcgcat gaaaaagatc cagatgccga tgaaaagaat ccagggcagg
     7201 atgtaaatca gactgctcca gatgctcggc ccgctcttct tgaaaatgac ctcgacatcg
     7261 tatttctgga tccattcgtc aaaggtctcg aggctggcgc gcggaggcag gatgacctga
     7321 aagccgcgct gcccctcgag ggcggcatcg ggcttgagtt ggccgtggat gacatcatcc
     7381 tcgaccttga tgctttccac cttgcccatc tggagatagt tcttgaaacc gtcgtaggtg
     7441 agcacgcttt cctgcggttc ctggcgggcg aactgctgcg agatcgccag ggcaagaaaa
     7501 atcagtccta cccagatcag cagcggccgg agcatgcggc cccactgcgg gccttcgggc
     7561 tgactgcgtt ggggcccggc cggccgccgc ggcggccctc cctggcgctg cgggggctgc
     7621 gaatctcctc ccccatcctg ccggtgcatt ggctggtcca tatcttgcgt atggtcgtac
     7681 aaatcgatat cctctcttaa tccatcacat agacggccgg gagattacgc agaatctgtt
     7741 tgtaatccag gccgtagccg acgacaaatt cattggcgat gctgaaaccg acatagtcga
     7801 tcgggatgtc ggtctcgaca ttggccggtt tgatgagcaa tgtgacgaag cgcagcgacg
     7861 cgggctcgag ggccatgaac ttttcatgca ggaacttgat cgagccgccg gtgtcgacga
     7921 tgtcctcgac gagcaggata tgccgtccct tgacgtcggc gttgatgtcc ttgagcatac
     7981 gcacgatccc ggacgagacc ttggcgttgc catagctgga gatcttgata aaatcgacct
     8041 ccagatcgat ttcgacatgg cggatgaggt cggccatgaa gagaaaagcc ccgttgagca
     8101 ccccgatcat gatgggctgc agcccgcggt aatcctcggt gatctggcgg cccacctcgc
     8161 ggatgcgggc gtctatcgcc tccgccgtga gcagggtgcg aaagccgccg tcatagacca
     8221 ctttttcgaa ttcagagcgc tgagtcacgg tcgatttcca ccttcagctg aaaaatgttc
     8281 gtggtgcgtg gggttacgcg gaacgggccg gcggcgcgga ggccgcagat ccagacgatt
     8341 tcaccgccgc tgcagagcag gtaaatgccg ccccgttcat agacgggcac tcctgcaccg
     8401 gcgagcaggt cggcgacctt cttgctcccc ttcagtccca gcggctgcat gcggtcgccc
     8461 ggccgggggg ggcgaagctc cagcgtcgac tgcaccagat cggcgtcgat ccaggcgatg
     8521 cggtggtcgc tgtttttctc tatcagctct aacggcggcg aaacccggtt tatttcaagg
     8581 gtcaaaccct cgcggagggg atagcggccg ggtgcggccg gtatggccat cgccgcggcg
     8641 gtgggccgct tggccagcag caccagccgg gtttcgctct gccagaactc gccggaaccc
     8701 gcggccaggc cgcgatctgt gcgcccggcg gcgaggaact gctcccagcc ggaccaaaag
     8761 gcctcgtcga gatcacggcc atccccgccg atgccctccc aggcctcgcg caggatatat
     8821 ttctgcagaa gagtaaagta cgataaaaag gcttcttttt caagaataat tttcccgctc
     8881 tttgccacga cggtgcagct cggcagggct gcgcgggcct ggctgcgcat ataagagtcg
     8941 acttcgcgca tatggccggc ggtgcggccc agcacagctt cgatggcggg attgtactcc
     9001 tccaacaggg ggagcagctc gtggcggatg cggttgcggc ggatgcggcg gtcggcgttg
     9061 gtggtgtcgg tgcagtactc cagcccccgg gcggcggcat gggcggcaat ctcggcgcgg
     9121 ctggcgaaga gcagggggcg gatgagcagg agcggggcgg gagggccagc gccgggcgat
     9181 gcggcggtgc agggcagatc cggcgccgca gcgatcgcgc tgcgggggac gatgccggcc
     9241 aggccgcgca ccccggcgcc gcggatgagg cggtcgagca cggtctcggc ctggtcgccg
     9301 gcgtgatggg cggtggcgat ggccgcgcag ccgcaccgac gggcgctggc gatgagaaaa
     9361 tcgtaacgca gggcgcgggc ggcggcctcg aggctcaaat ggtgagcgcg ggcgtaaaca
     9421 cgcacatcgc cccggccgac ctcgcagggg agggcgcggc cggcgcagag ggtgcgcacg
     9481 aaatcctcat cacggtcggc ttcggcgccg cgcagctgat ggttgaaatg ggcggcggtc
     9541 agcgccagac gccactccgg cgccagctgg gcgagaagat cgagcagcac caccgaatcg
     9601 agcccgcccg agagggcgac cagcacccgc atgcccggct gcagcaggtt gtgctcacgg
     9661 atatacgcga tgaatctgtc gaacatctgt gttgtgccct cgcctctcgt tcccaccccg
     9721 ctcacttttt cacggccggg ttccctcgct tctcgttccc accccgttca ctttttcacg
     9781 gccgagttcc ctcgcctctc gttcccaccc cg
//
LOCUS       DAOPWU010000108         9703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7056_length_9703_cov_4.672782, whole genome shotgun sequence.
ACCESSION   DAOPWU010000108 DAOPWU010000000
VERSION     DAOPWU010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7056_length_9703_cov_4.672782"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKM23_07205"
     CDS             complement(<1..600)
                     /locus_tag="PKM23_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNY91300.1"
                     /translation="MKRDFLAETDFSKKEIIETFKLARKLKKKVKKGEKHHLLRGKVL
                     GMIFQKPSNRTRVSFEVGMYQLGGHAIYLGPAEIGLGTRESVADVARVLSRFTDGIMA
                     RVFGHDLVEGLAQWAPVPVINGLSDLLHPCQILGDLLTIIEHKGTFEGVRIAYVGDGN
                     NIVNSFINVAGILPLDLRVACPKGYEPNAEILAAARAKAV"
     gene            complement(616..1986)
                     /gene="hslU"
                     /locus_tag="PKM23_07210"
     CDS             complement(616..1986)
                     /gene="hslU"
                     /locus_tag="PKM23_07210"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNY91301.1"
                     /translation="MEMTPQQIVHELDKYIIGQDEAKRAVAIALRNRWRRQAVPDDLR
                     EEIMPNNIILIGPTGVGKTELARRLARLDSAPFIKVEASKFTEVGYVGRDVESIVRDL
                     VDLAVNMVRSERLDQVQESAREQAIEHILDILLPGSRRENGEGGETATEKRGTAGGAE
                     EARTRRSRKKMRRLLLEGALDDRLIEMEVSSEITPMMQVISPVGIEEMGLSLQDLFGP
                     FIPKKSKKRKMSVAEAVMLQVQEETQKLIDMDDVVREAIYRVEETGIVFLDEIDKIAG
                     EGDGMGPDVSRSGVQRDLLPVIEGTNVITKYGMVRTDHILFIASGAFHVAKPADLIPE
                     LQGRFPIRVELHSLNARDFERILVEPRNALTRQYIALLGTEGVELTFTRGAISEIARI
                     ASEANERAENIGARRLHTILTILLEDYLFQLPDLELKAIRIDKRTVQERLREVIADED
                     LSRYVL"
     gene            complement(1989..2549)
                     /gene="hslV"
                     /locus_tag="PKM23_07215"
     CDS             complement(1989..2549)
                     /gene="hslV"
                     /locus_tag="PKM23_07215"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNY91302.1"
                     /translation="MKAVQMSYLKPVHATTIIGLRHKGVAVLAGDGQVTYGDMVLKTG
                     ARKVRKLYHDQVLAGFAGGAADAFTLFERFEAKLDEFNGNLPRSAVELAKDWRMDRYL
                     RRLDAQLVVLNKEKIFLLSGTGDLIEPDDTVIAIGSGGPFALAAARALLAHTRLSALQ
                     IAREAMEIAAGICIFTNDKLTIETLE"
     gene            complement(2598..4028)
                     /gene="rpoN"
                     /locus_tag="PKM23_07220"
     CDS             complement(2598..4028)
                     /gene="rpoN"
                     /locus_tag="PKM23_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNY91303.1"
                     /translation="MVQISQKLVMQLKQSPQQVLLSSLLQLPVLSLEQRIRLELETNP
                     LLELETEEELAQELDENAEQTLEQEEPDEEVDPAEKKDTAEKEEIDWEEILNDEESYE
                     VRVPREKNVDTYERPEVYQESLTDHLITQLNLSPLTAEEQVIGEYIIWNINPVGYLGT
                     PVEAMAENLQLDAATVERILGVIQRFDPVGIGARDLRECLLVQLLEEQPHNEIAIEII
                     RDHFEDFKNKRFEKLAKDLGLDLETIKEIFSHITRLNPKPGEGYASLVDNYIVPDLEV
                     REEDGEFKVYMHDWNVPQLRINNEYRKMMLDRKHTSKETRDYIKNRLESARWLINSIH
                     QRRATILRVMETIIEKQHDFFLHGPEKLKPLILKDIADEIGMDISTVSRVTNGKYVQT
                     EWGLFELKYFFSEKIHSDEGEDVSNKIIKNLIKEIITAEPPKKPYNDQKIAEMLKKKG
                     YNVARRTVAKYREQMGLPVSRLRRKI"
     gene            complement(4131..4868)
                     /gene="lptB"
                     /locus_tag="PKM23_07225"
     CDS             complement(4131..4868)
                     /gene="lptB"
                     /locus_tag="PKM23_07225"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNY91304.1"
                     /translation="MADVPILRCTGLNKIYGKRRVVDQVSIEIRKGETVGLLGPNGAG
                     KTTTFYMIVGMIRPNSGRIFIDDQDITALPMYRRARMGIGYLPQEASVFRRLTVEENI
                     LAILETLPLNRSQRHERCAQLLDDLGITRVAKSKAFTLSGGERRRVEICRALVTNPGF
                     MLLDEPFAGVDPLAVEDIQHIVADLKKRGIGVLITDHNVHETLSITDRAYLLYEGKVL
                     KSGSAEFLANDEEARKLYFGEKFKLDR"
     gene            complement(4883..6037)
                     /locus_tag="PKM23_07230"
     CDS             complement(4883..6037)
                     /locus_tag="PKM23_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNY91305.1"
                     /translation="MKQAVRLFCAACCMLPALRLQGQSNTLQLVHADSARGFIENGRV
                     VRELMGSVEFMQDSSRMFCDRALQYPEEKLALFSGRVRIITPPRELQAEQIYHYEVRR
                     EQIASGRARLITPRQLLTADALHYFEKEERALAEGHAVISDSSERAYLSGARIEYLRL
                     KGYARVDGDPLFVRHDSSGTDSMTVRSRLMEMFEDGERILAREEVTITQGEVSARCGE
                     MTFMRNGEKVLLTLQPEARRAADFLRGSEIDLYLDQRVLTGIGIRGSAAVVTRVDTLA
                     SPKPRYDLISGEEIYVGIKKNSIDSVHVQGRATSYYHLFDDRNYQGFNKVLGDELMMS
                     FEEEKVEWVGVKSAPAASNGIFYPAGQERPLLKELEPLLQPFGLQPESPE"
     gene            complement(6034..6741)
                     /gene="lptC"
                     /locus_tag="PKM23_07235"
     CDS             complement(6034..6741)
                     /gene="lptC"
                     /locus_tag="PKM23_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNY91306.1"
                     /translation="MNAGKSSPVRAPEMILRTPRLFCCRRRPGLALLVALALLAAAPA
                     CQKHKPDAPVEEQQEEQPDQEGWNSTVHATKNGRPEAIVTYGHMKRFNNRKKVYFDEG
                     VKVDFYDPEGRMRSRLEAENGEMNEATNDIMARGSVVVVSDTGITVFSESLGYRQATG
                     KIYSDVDVVLISDKGDTLYGRGFESDTRMRNWRIRALSGASHARIDLSGERFKRRPSF
                     KPDSSAVRPGDSTGVRR"
     gene            complement(6704..7612)
                     /locus_tag="PKM23_07240"
     CDS             complement(6704..7612)
                     /locus_tag="PKM23_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNY91307.1"
                     /translation="MAPLAKRIKNDLIYGLVVGLIALLRLLPRGSAAALMRGLAGAGF
                     RLARKEREKTIRHLTLAYGSEKSPAQIEALAGAVWSHFGVMAVDAIRLPRILAAGINT
                     LVEAQGVESLRSALGRGRGVIVLTGHFGNWELFGAWVAQNGFPVKVVGTSAYDPRLDR
                     IIVGLRNAAGYTNIARGKGTREIIRTLRDNEVLGMLIDQDTRVDGVFVNFFGRAAHTA
                     VGPVILAQKMGCALLPAFIHLKEDGRYLIEFDEEITLTTTGDAAQDQVVNTQKISDAY
                     ERAIRRHPEQWVWMHERWKKQPGEGA"
     gene            complement(7612..8688)
                     /locus_tag="PKM23_07245"
     CDS             complement(7612..8688)
                     /locus_tag="PKM23_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNY91308.1"
                     /translation="MRRACGPSTGSKTCSFRRRGSTIFAASWRSAIRPEDENIDPVAT
                     AREVLRIESEAVAALAPRVGEAFARAVELILACKGRVIITGLGKSGIVGKKIAATLTS
                     TGTSAYFLHPVEAVHGDLGMVLKNDVVICISKSGNTDEITKLFPVLKQIGVPVITMTG
                     NLRSALAQRSDVVLDVGVAEEACPLDLAPTASTTATMAMGDALAVALLKKRQFCSEDF
                     AFLHPGGSLGKRLLKVSELMFGGEHIPAVPPEATLREVIFEITRKRFGCTCVVEPSGR
                     LAGLITDGDLRRTMQDHENPGALHAAEIMNPAPKTIGPESRALQALQVMKHFNIMQLV
                     VTDGEAHPVGMLHLHDLLDAGIGS"
     gene            complement(8585..9427)
                     /gene="kdsA"
                     /locus_tag="PKM23_07250"
     CDS             complement(8585..9427)
                     /gene="kdsA"
                     /locus_tag="PKM23_07250"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNY91309.1"
                     /translation="MKTIKVGSISIGPNEPLALIAGPCVIESESLVLRTAEAVQKIAS
                     RLGMPYILKSSYTKDNRSSATSFQGFGLDEGLRILEKARLELGVPVLSDVHNEHEAII
                     AGGVLDVIQIPAYLSMQTSLALAAARTGRAINVKKGQFLDPVDMKNVIAKIEGEGNEN
                     IMITERGTFFGYHNLVIDYRSLPIMRSFGYPVVIDPTHAIRVYGISSSDPAGGNPQYV
                     PALTRAALAAGCEVLFVETHPNCSEALCDAASMWPLDRLENLLIQAKRIDDLRRELEV
                     RHPA"
     gene            complement(9414..>9703)
                     /locus_tag="PKM23_07255"
     CDS             complement(9414..>9703)
                     /locus_tag="PKM23_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282496.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNY91310.1"
                     /translation="EISERHRHRYEVNNSYLERLTGEGLIVAGRNPESGLVEIIELAG
                     HPWFVGVQFHPELKSRVLAAHPLFREFVAAALAYKLDMKSSEEKDILHEDH"
BASE COUNT         1684 a         2954 c         3101 g         1964 t
ORIGIN      
        1 caccgctttg gcgcgcgcgg ccgccaggat ctcggcgttg ggctcgtagc ccttggggca
       61 ggccacgcgc aggtcgaggg gcaggatgcc ggcgacattg ataaaggagt tgacgatatt
      121 gttgccgtcg ccgacgtagg cgatccgcac cccctcaaag gtgcccttgt gctcgatgat
      181 ggtgaggagg tcgccgagga tctggcaggg atggagcaga tcggagaggc cgttgatcac
      241 cggcaccggc gcccactgcg ccagtccctc gaccaggtcg tggccgaaga cgcgcgccat
      301 gatgccgtca gtgaaccggg agaggacgcg ggccacatct gcgaccgact cgcgcgtgcc
      361 gagcccgatc tcggccggac cgagataaat ggcatggccg ccgagctggt acatccccac
      421 ctcgaaggag acccgggtgc ggttggaggg cttttggaag atcatgccca gcaccttgcc
      481 gcgcaggaga tggtgctttt cgcctttttt gacttttttc ttcagcttgc gggcgagttt
      541 gaaggtctcg atgatctctt tcttggaaaa atcggtttca gccagaaaat ctcgtttcac
      601 gggaacctcc tttatttata aaacatagcg gcttaaatcc tcatcggcga tcacctccct
      661 gagccgctcc tgcaccgtcc ttttgtcgat acggatggcc ttgagctcga gatcgggcag
      721 ttgaaagaga tagtcctcga gcaggatggt cagaatggta tgaagacggc gtgcgccgat
      781 attctccgcc cgttcgttgg cttcggaggc gatgcgggcg atctcgctga tcgccccgcg
      841 cgtgaaggtc agctccaccc cctcggtgcc gagcagggcg atgtactgcc gggtcagggc
      901 gttgcggggt tcgaccagga tgcgctcgaa atcgcgggcg ttgaggctgt gcagctcgac
      961 gcggatcgga aagcggccct gcagctcggg gatgaggtcc gccggcttgg cgacgtgaaa
     1021 ggccccggag gcgataaaaa ggatgtggtc ggtccggacc atgccgtact tggtgatgac
     1081 attggtcccc tcgatgaccg gcagaagatc gcgctgcacc ccgctgcgcg agacatccgg
     1141 ccccatgccg tcgccctcgc cggcgatctt gtcgatctcg tcgagaaaga cgatgccggt
     1201 ctcctcgacc cggtagatcg cttcgcggac gacatcgtcc atgtcgatca gcttctgggt
     1261 ctcctcctgc acctgcagca tcaccgcctc ggccacgctc atcttgcgct ttttgctctt
     1321 tttcgggata aagggtccga agagatcctg caggctgagc cccatctcct cgattcccac
     1381 cggggagatg acctgcatca tcggggtgat ttcgctggag acctccattt cgatgaggcg
     1441 gtcgtcgagc gcaccctcga ggaggaggcg gcgcattttt ttacgcgagc gccgggtgcg
     1501 cgcctcttcg gcccccccgg cggtgcccct tttttccgtg gccgtttcgc ccccctcgcc
     1561 gttctcacgc ctggagccgg gcagcagaat gtcgaggatg tgctcgatgg cctgttcgcg
     1621 ggcgctctcc tgcacctggt ccaggcgctc gctgcgcacc atattgacgg ccaggtcgac
     1681 gaggtcgcgg acgatcgatt cgacatcgcg gccgacatag cccacctcgg tgaacttgct
     1741 ggcctcgacc ttgatgaagg gggcgctgtc gagacgcgcg aggcggcgcg ccagttcggt
     1801 cttgccgacc ccggtgggcc cgatcaggat gatgttgttg ggcatgatct cttcacgcag
     1861 atcgtccggg accgcctgcc ggcgccagcg gttgcgcagg gcgatggcga cggcccgttt
     1921 ggcctcatcc tggccgatga tgtacttgtc cagttcatgg acgatttgct gcggggtcat
     1981 ttccatggct attccagggt ctcgatggtc agtttgtcgt tggtgaagat gcagatgccc
     2041 gcggcgatct ccatcgcctc cctggcgatc tggagggccg aaagccgggt atgggccagc
     2101 agcgccctcg cggcggccag cgcgaagggg ccgcccgagc cgatggcgat gacggtgtca
     2161 tcgggctcga tgagatcgcc ggtgccggag agcagaaaga tcttttcctt gttgaggacg
     2221 accagctggg cgtcgaggcg gcgcagatag cggtccatgc gccaatcctt ggccagctcc
     2281 accgccgaac gcggcaggtt gccgttgaat tcgtcgagct tggcctcaaa gcgctcgaaa
     2341 agggtaaagg catcggcagc cccgccggca aaaccggcca gcacctggtc gtgatagagc
     2401 ttgcgcacct tgcgcgcgcc ggtcttgagc accatgtcgc cgtaggtgac ctggccgtcg
     2461 ccggcgagga cggccacgcc cttgtggcgc aggccgatga tggtggtggc gtggactggt
     2521 ttgagatagg acatctgcac tgccttcatg ttggtcccgc ggaccgcctc cgctcgcagg
     2581 aggggccggg ggaggaatca gatcttgcgc ctcagccgtg aaaccggcag ccccatctgt
     2641 tcgcggtatt tggcgacagt gcggcgggcg acattgtagc cctttttctt gagcatctcg
     2701 gcgatctttt gatcgttgta gggctttttc gggggctcgg cggtgatgat ctccttgatc
     2761 aggttcttga tgatcttgtt ggagacatcc tccccctcat cgctgtgaat cttttcactg
     2821 aaaaagtact tgagttcaaa gaggccccat tcggtctgga cgtacttgcc gttggtgacg
     2881 cggctgaccg tggagatgtc catgccgatc tcgtcggcga tgtccttgag gatgaggggt
     2941 ttgagctttt ccgggccgtg gaggaaaaag tcgtgctgct tttcgatgat cgtttccatg
     3001 acgcgcagga tggtggcgcg gcgctggtgg atggagttga tcagccagcg cgctgattcg
     3061 aggcggttct tgatatagtc ccgggtctcc ttgctggtgt gcttgcggtc gagcatcatc
     3121 ttgcggtatt cattgttgat gcgcagctgg ggcacattcc agtcgtgcat atagaccttg
     3181 aattcgccat cctcctcgcg cacctcgagg tccgggacga tatagttgtc caccagcgag
     3241 gcgtagccct cgccgggttt gggattgagc cgggtgatgt ggctgaaaat ctccttgatg
     3301 gtctcaagat cgaggccgag atccttggcg agcttttcga agcgcttgtt cttgaaatcc
     3361 tcaaaatggt cgcggatgat ttcgatggcg atctcgttgt gcggctgctc ctcgagaagc
     3421 tgaacgagca gacactcgcg caggtcgcgg gcgccgatgc cgaccggatc gaagcgctgg
     3481 atgaccccga ggatgcgctc gaccgtggcg gcatcgagct ggaggttctc ggccatggcc
     3541 tcgacggggg tgcccagata gccgacgggg ttgatgttcc agatgatata ctccccgatg
     3601 acctgctcct cggcggtgag gggcgaaagg ttgagctggg tgatgaggtg gtcggtcagg
     3661 ctctcctggt agacctcggg ccgctcatag gtatcgacat ttttttcacg gggaacgcgg
     3721 acctcatagc tctcctcgtc gttgaggatc tcctcccagt cgatctcctc cttctcggcg
     3781 gtatccttct tctcggcagg atcgacctcc tcgtcgggct cctcctgttc gagggtctgc
     3841 tcggcattct catccagttc ctgggcgagt tcctcctcgg tctcaagttc gagcagggga
     3901 ttggtctcca gctcgaggcg tatacgctgt tcaagactga gcaccggcag ctgcagcagc
     3961 gaggagagca gcacctgctg cggcgactgc ttgagctgca tgaccaattt ttgggaaatt
     4021 tgtaccatgg tttaaagctc caagacctgg cgttgcgatg atgttaaaac tgatgatgcg
     4081 gttagctgta acttttctcc ccggccgcca gcggcggccg gcacaacccg ttacctgtcc
     4141 agtttgaatt tttccccgaa gtaaagttta cgcgcctctt cgtcattggc caggaattca
     4201 gcactgcccg atttcagcac cttgccctcg tagagcagat aggcgcgatc ggtgatcgaa
     4261 agggtctcgt gtacattgtg gtccgtgatc agcaccccga tgccgcgctt cttgagatca
     4321 gcgacgatgt gctggatatc ctcgacggcc agggggtcga ctccggcgaa tggctcgtcc
     4381 aggagcataa agcccggatt agtgaccagc gcccggcaga tctccacccg ccgccgctct
     4441 ccgcccgaga gcgtgaaggc cttgcttttg gccacccggg tgatgccgag gtcgtcgagc
     4501 agctgcgcgc agcgctcatg acgctgactg cggttgagag gcagggtctc gaggatggcg
     4561 aggatgttct cctcgacggt caggcggcgg aagaccgagg cctcctgcgg cagatagccg
     4621 atgcccatgc gcgcgcggcg gtacatgggc agtgcagtga tatcctgatc gtcgatgaag
     4681 atgcggcccg agttgggacg gatcatgccg acgatcatat agaaggtggt ggtcttgccc
     4741 gccccgttgg ggccgagcag tccgacggtc tcgcctttgc ggatctcgat gctgacctga
     4801 tccaccaccc gccgcttgcc gtagatcttg ttcaggccgg tgcagcgcaa tatcggtaca
     4861 tctgccatgg cgtgctctct gtttattccg gcgattccgg ctgcagtccg aagggctgaa
     4921 gcaggggctc caactccttg aggagtggcc gctcctgccc tgcgggataa aagatgccat
     4981 tggaggcggc cggagcgctc ttgaccccga cccattcaac cttttcctcc tcaaagctca
     5041 tcatgagctc gtcgcccagc accttgttga aaccctgata gtttctatcg tcgaaaagat
     5101 gataatagct cgtcgcccgc ccctggacgt ggacggagtc gatgctgttc tttttgatcc
     5161 cgacatagat ctcttcgccg ctgatcaggt catacctggg cttggggctg gccagggtgt
     5221 cgacccgggt caccaccgcc gcgctgccgc gaatgccgat gcccgtcagg acccgctggt
     5281 cgagatagag atcgatctcg gatccgcgca gaaaatcagc agcgcgtctg gcctccggct
     5341 ggagcgtgag cagcactttt tccccgttgc gcataaaggt catctcgccg cagcgggccg
     5401 agacctcccc ctgcgtgatg gtcacctctt cgcgcgccag gatgcgctct ccgtcctcga
     5461 acatctccat gagccggctg cgcacggtca tgctgtccgt cccgctcgaa tcatggcgga
     5521 cgaagagggg atcgccgtcg acgcgggcat agcccttgag gcggaggtac tcgatgcgtg
     5581 cgcccgagag gtaggcccgt tccgacgaat ccgagatcac ggcatgcccc tcggccagag
     5641 cccgctcctc cttttcgaag tagtggaggg catctgcggt gagcagctgc cggggggtga
     5701 tcaggcgcgc ccggccgctg gcgatctgct cgcggcgcac ctcataatgg tagatctgct
     5761 cggcctgcag ctcgcgcggc ggtgtgatga tcctgaccct gccgctgaac agggccagct
     5821 tttcttcagg atactgcagg gcgcggtcgc agaacatgcg gctcgagtcc tgcatgaact
     5881 ccacggagcc catcagctcg cgcaccacgc ggccgttctc gataaaaccg cgcgccgaat
     5941 cggcgtggac gagctggagg gtgttgctct ggccctgcag ccgcagggcc ggcagcatgc
     6001 agcaagccgc gcaaaacagc cgcaccgcct gcttcatcgc ctcactcccg tcgaatcgcc
     6061 cggccgtacg gcgctcgaat cgggcttgaa ggaaggccgc cgtttgaaac gctcgcccga
     6121 gagatcgatg cgcgcgtggc tcgccccgga aagcgccctg attcgccagt tgcgcatgcg
     6181 ggtgtccgac tcgaagcccc tgccatacag ggtgtcgccc ttgtcgctga tcagcaccac
     6241 atcgacatcc gagtagattt tgccggtggc ctggcggtag cccaggctct cggaaaaaac
     6301 cgtgatgccc gtatccgaga ccacgaccac gctgccgcgg gccataatgt cgttggtggc
     6361 ttcgttcatt tcgccgttct cggcctcgag gcgcgaacgc atgcgccctt cgggatcgta
     6421 gaaatcgacc ttcacccctt catcaaaata gaccttttta cggttgttga agcgcttcat
     6481 gtggccatag gtcacgatcg cttcggggcg gccatttttg gtggcgtgga cggtcgagtt
     6541 ccatccctcc tggtccggct gctcctcctg ctgctcctca accggagcat cgggcttgtg
     6601 cttctggcag gcgggagcgg cagcgagcag cgcgagggcc acaagcaaag ccaggcccgg
     6661 ccggcgccgg cagcagaaga gacgtggcgt gcgcaggatc atctcaggcg ccctcaccgg
     6721 gctgcttttt ccagcgttca tgcatccaga cccactgttc gggatggcgg cggatggcgc
     6781 gctcataggc atcagaaatt ttctgggtgt tcaccacctg gtcctgggcc gcatcgcctg
     6841 tggtagtaag agtgatctcc tcgtcgaatt cgatcaggta gcggccgtcc tccttgaggt
     6901 ggataaaggc cggcagcaag gcgcagccca ttttttgtgc caggatcacc gggccgacgg
     6961 cggtatgcgc cgcacggccg aaaaagttga caaaaacacc gtccacgcgg gtatcctggt
     7021 cgatgagcat gcccagcacc tcgttgtcgc gcagggtgcg gatgatctcg cgcgtcccct
     7081 tgccgcgggc gatgttggta tagccggccg cattgcgcag gccgacgatg atccggtcca
     7141 ggcgcggatc ataggcggag gtgcccacca ccttgaccgg gaagccgttc tgcgccaccc
     7201 aggccccgaa aagttcccag ttgccaaagt ggccggtcag gacgataacg ccccggcccc
     7261 gtcctaaagc cgagcgcagg ctctccaccc cctgcgcctc gacgagggtg ttgatgccgg
     7321 cggcgaggat gcgcggcagc cggatggcgt cgacagccat cacgccaaaa tggctccaga
     7381 ccgccccggc cagggcctcg atctgcgcgg ggctcttttc ggagccatag gccaaggtga
     7441 gatggcggat ggtcttttcc cgctccttgc gcgccaggcg gaatcccgct ccagccaggc
     7501 cgcgcatcag ggccgctgcg ctgccgcgcg gcaaaaggcg cagcagggcg atgaggccaa
     7561 ctaccagtcc gtagatgaga tcgtttttga tccttttcgc gagcggggcc atcaggatcc
     7621 gatcccggcg tcaagcaggt catggagatg gagcatgccg accggatgcg cctccccgtc
     7681 ggtgaccacc agctgcatga tattgaaatg cttcatcacc tgcagggcct ggagggcgcg
     7741 gctctcgggg ccgatggtct tgggcgcagg attcatgatc tcagcggcgt gcagtgcgcc
     7801 tggattctcg tggtcctgca tggtgcggcg caggtcgccg tcggtgatca gtcccgccag
     7861 ccggcccgag ggctccacca cacaggtgca gccgaagcgc ttgcgcgtga tttcgaaaat
     7921 cacctcgcgc agggtcgcct ccgggggaac ggccgggatg tgctccccgc cgaacatcag
     7981 ctccgaaacc ttgagcagcc tcttgccgag cgagccgccg ggatggagaa aggcgaaatc
     8041 ctcggaacag aactgccgct tcttgagcag ggccaccgcc agggcatcac ccatcgccat
     8101 ggtcgcggtc gtgctggctg tgggagccag gtcgaggggg caggcctcct cagcgacccc
     8161 gacatcgagg acgacatcgc tgcgctgggc cagagccgaa cgcagattgc cggtcatggt
     8221 gatgactggc accccgatct gcttgagcac cggaaaaagc ttggtgattt cgtcggtatt
     8281 gccgctcttg gaaatgcaga tcacgacatc attcttcaag accatgccaa ggtcgccgtg
     8341 aaccgcctca accggatgga gaaaataggc cgaggtgccg gtggaggtga gggtggcggc
     8401 gatctttttg ccgacgatcc ccgacttgcc caggccggtg atgatcaccc tccccttgca
     8461 ggccaggatc agctcgaccg cccgggcgaa ggcctcaccc actcgcggcg ccagtgcggc
     8521 caccgcctcg ctctcgatgc gcagcacctc cctggccgtg gcgaccggat cgatattttc
     8581 atcctcaggc cggatggcgg acctccagct cgcggcgaag atcgtcgatc ctcttcgcct
     8641 gaatgagcag gttttcgagc cggtcgaggg gccacatgct cgccgcatcg caaagggctt
     8701 cggagcagtt gggatgggtc tcgacgaaaa gcacctcgca gccggccgcc agggccgcgc
     8761 gcgtcagggc cggcacatac tggggattgc ccccggccgg atcgctcgag gagatgccgt
     8821 agacgcggat ggcatgggtc ggatcgatga ccaccggata gccgaaactg cgcatgatgg
     8881 gcaggctgcg ataatcgatg accagattgt gatagccgaa aaaggtgccg cgctcggtaa
     8941 tcatgatgtt ctcgttgccc tcgccctcga tcttggcgat gacattcttc atgtcgacag
     9001 gatcgaggaa ctgccccttc ttgacgttga tcgcccggcc ggtgcgcgcc gcagcaaggg
     9061 ccagcgaggt ctgcatggaa agataggccg ggatctggat gacgtcgagg accccgccgg
     9121 cgatgatcgc ctcatgctcg ttgtggacgt ccgagagcac cggaaccccc agctcaaggc
     9181 gcgccttttc gaggatacgc agcccctcgt ccaggccgaa gccctgaaaa ctggtcgccg
     9241 aggagcggtt gtccttggta taggaggatt tcaggatata gggcatgccg agccgggagg
     9301 cgatcttttg caccgcctcg gccgtgcgca gcaccaggga ttcgctctcg atcacacagg
     9361 gaccggcgat gagcgccagc ggctcgttgg ggccgataga gatcgaaccc accttaatgg
     9421 tcttcatgga ggatgtcctt ctcttcagaa gatttcatgt ccagtttgta ggcgagggcg
     9481 gccgcgacaa actcccggaa aaggggatgg gcggcgagca cccggctctt gagttcgggg
     9541 tggaactgca ccccgacaaa ccaggggtga cccgccagtt cgatgatctc gaccagaccg
     9601 ctctccggat tgcggccggc gacaatcagc ccctccccgg tcagcctctc cagatagctg
     9661 ttgttgacct cgtagcggtg gcggtggcgc tcgctgatct ccc
//
LOCUS       DAOPWU010000109         9652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7103_length_9652_cov_4.515578, whole genome shotgun sequence.
ACCESSION   DAOPWU010000109 DAOPWU010000000
VERSION     DAOPWU010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7103_length_9652_cov_4.515578"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1743
                     /locus_tag="PKM23_07260"
     CDS             181..1743
                     /locus_tag="PKM23_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HNY91311.1"
                     /translation="MPDDKGLILWVDDEIDLLRPHVLFLEEKGYSVTTVTNAEDALEL
                     VRGANFDLILLDEMLNGMDGLSALNEFKNIRPGLPVIMVTKNEEESLMEQAIGGKIDD
                     YLTKPVNPSQILLVCKKILQKRKIAGERISRDYTSEFNQIAMRLMGGMEWQDWIDVHL
                     RLCEYDIELDQHPDLGLKQILYDQKRECNAEFGRFVEKNYLAWLGAAGAPPLSVDIVP
                     RYVLPHVRAGRRTLLMVLDNLRLDQWLVVEPLLREFFNIRREYYFSILPTATPYARNA
                     IFSGLFPSEIEKQLPELWTQGDENDDVSCNRFESQLLEEQLRRAGVELKPGPKYAKVL
                     DMQEHRLIARKIQEFGDLPLSSIVINFVDILAHSRSSSELIKEMIPNESAFRSLTKSW
                     FEHSHMYLALQQLAQSGSTVIITSDHGSIRGLRGAKVIGDRETSTSLRYKYGRNIKAD
                     AKNAMVIRDPRQYRLPVRGVNTNYLIAKEDFYFVYPTNYHYYLNYYADSFQHGGISME
                     EMVLPVITMEPK"
     gene            1756..2274
                     /gene="tsaE"
                     /locus_tag="PKM23_07265"
     CDS             1756..2274
                     /gene="tsaE"
                     /locus_tag="PKM23_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNY91312.1"
                     /translation="MQESRYQLLESWITRNADETLARGVIIAESLTPGEMLVFTGNLG
                     SGKTTLIQGICRGLGVEETVTSPTFTLINEYRGRLPVYHFDFYRLHSAVELTDLGLEE
                     YLYGDGICLVEWPELIAEWLPVRHIRLHLEHGIHAAGRINALQVEQQLEPVPITPDVR
                     LIEVYRHEPARD"
     gene            2255..2944
                     /gene="tsaB"
                     /locus_tag="PKM23_07270"
     CDS             2255..2944
                     /gene="tsaB"
                     /locus_tag="PKM23_07270"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNY91313.1"
                     /translation="MNLLAIESATPVCAAALTRGETLVVEYRAAIRNAHGRLLTRAID
                     RLLADAGWQPGDLEALAVSIGPGSFTGLRIGLALAKGIALAREIPIAAVPTLEVLAAQ
                     APVERGLIAPLVRSRADEYYVALYERSDAGLAALQEVQILPWGEIPRAVPPEAMLIGG
                     APEQPLPSGYRSAPAASALPGALTVARLGWQRLQEGRRENPDLLEPLYLQEFIVGKPK
                     QPVFSVEGHVR"
     gene            2934..3452
                     /gene="rimI"
                     /locus_tag="PKM23_07275"
     CDS             2934..3452
                     /gene="rimI"
                     /locus_tag="PKM23_07275"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNY91314.1"
                     /translation="MFADHFLPGVLELPTLARPLHYRAMREADLPAIAVIERQLFSSP
                     WSEELFREDLDQEYALSLVLTDAESIEGYMITFLVLDEMHIANVAVAPAYQRRGIAFE
                     VLRRLLVAGRRMHYFLAHLEVRQSNLGAIALYERLGFAKVGLRKNYYENEHEDAVLMS
                     CLLQSNPLLAAD"
     gene            3486..4511
                     /gene="accD"
                     /locus_tag="PKM23_07280"
     CDS             3486..4511
                     /gene="accD"
                     /locus_tag="PKM23_07280"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNY91315.1"
                     /translation="MEWFKRVTPGLEAQTKKELPDGLWIKCDRCGEILYKKELERNRM
                     VCTGCAFHFRMDSGQYIALLADEGSFEEFNENLSSADPLHFKDSKKYTDRLEEAMAKT
                     GLKEAVRTGECTLAGLPVVMAIMDFRFIGGSMGSVVGEKVSRAIDRAVETRRPLIILS
                     SSGGARMQEGALSLMQMAKTAAKLALLAEARIPFISILTDPTAGGTTASFSMLGDVII
                     AEPGAFIGFAGPRVIKQTIGQDLPEGFQRAEFVQEHGFVDMIVDRRQLKEQIERILVW
                     FVGPARKKPRRAAPKKKSAPEAAETVASAPKRGKAAGEKSAKRTGSSAAKPQTRKNST
                     TKPASEA"
     gene            4508..5272
                     /gene="surE"
                     /locus_tag="PKM23_07285"
     CDS             4508..5272
                     /gene="surE"
                     /locus_tag="PKM23_07285"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNY91316.1"
                     /translation="MRILLTNDDGINAQGLAALAAEMAKHGEIIVYAPASEMSAVGHA
                     ITLSDPLRFSECRKNGEFFGIAVRGTPADCVKVAIWDLGRQDRLPDIVLSGINQGSNT
                     GINTIYSGTVSAATEGAISGIPAVAISLASYTSGDFSGAAAFAPIIARQLLAHPLPPG
                     VFLNVNVPAKPLEQIRGVRITRQGMTNYREEYEIRKDPHSRNYYWLTGDKMEVEPDDE
                     IDDRAIIDDEISVTPVHCDMTYYPGLDMLRKWQISL"
     gene            5295..6833
                     /gene="guaA"
                     /locus_tag="PKM23_07290"
     CDS             5295..6833
                     /gene="guaA"
                     /locus_tag="PKM23_07290"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNY91317.1"
                     /translation="MEHLERILILDFGSQYTQLIARRVRELGVFSEIRPYSISPAELA
                     QIQPRGIILSGGPASVYGAEAPLPEPALLAQDIPLLGICYGLQVIGQLCGGEVGRAPQ
                     REYGRAELQIDETATLFDGLGDFLTVWMSHGDHLTRIPPDFSVIAHTVNAPIAAIRHR
                     HRPLYGIQFHPEVVHTPRGSELLANFVFKVCGCRGDWNASSFVERTVAEIRERVGGEQ
                     VLCALSGGVDSTVAAALVGRAVGRQLTCIFVDTGLLRENEAAEVCSFYRNADFEFRFV
                     DAGERFLSRLAGVTDPEQKRKIIGREFIAVFEEEAARLGGVGYLVQGTLYPDVIESVS
                     TRGPSATIKSHHNVGGLPEKMRLKLIEPLRELFKDEVRRAGRELQVPPFILGRHPFPG
                     PGLAVRILGEITPERLAILKPADRIFMEELRRSGEYDRIWQAFAVLLPVQTVGVMGDE
                     RTYENVIALRAVTSMDGMTADWAEIPRSLLALISNRIINEVKGVNRVVYDISSKPPST
                     IEWE"
     gene            6925..8757
                     /gene="glmS"
                     /locus_tag="PKM23_07295"
     CDS             6925..8757
                     /gene="glmS"
                     /locus_tag="PKM23_07295"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNY91318.1"
                     /translation="MCGIVGYIGSQNALPLLLNGLHRLEYRGYDSAGVALWTDEGIWM
                     QKAAGKIAELERILPAEPPQSTLGIGHTRWATHGEPNSVNAHPHCDCGRRIAVVHNGI
                     IENFPLLRDELIRKGHTFQSQTDSEVLCHLFEEYLERGMELGEAVRQGLACVQGTYGL
                     AIIDRDHPDTLVAARNGSPLVIGHAEEEFFLASDAAPLVGVIKSVTYLDDGELAVLTR
                     SGMTVRTIENCPVDKHSEPIDLDLQQIEKSGYAHFMLKEIMEQPSTLADTLRGRLMWE
                     EGTARLGGIEHDFESLLYARRIVLTACGTSWHSALIGEFMFEEMLGIPVEVEYASEFR
                     YRNPIVNDDSVVIAISQSGETADTLAAIREAKRKRSKVYGICNVVGSSIARETDAGVY
                     LHAGPEIGVASTKAFTSQVTVLALITLLLGRMRSISSARGRELVSELQAIPGKIRTIL
                     EQHEEIRQIAEIHQYVRNCLYLGRGYNYPVALEGALKLKEIAYVHAEGYPAAEIKHGP
                     IALIDNQMPVVVIATRDSIYEKILSNIEEVRARSGRVIAIATEGDQEIKKHAQYVIYV
                     PQTIEMLSPLLTIIPLQLFAYYMAILRNCDVDQPRNLAKSVTVE"
     gene            8819..>9652
                     /locus_tag="PKM23_07300"
     CDS             8819..>9652
                     /locus_tag="PKM23_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91319.1"
                     /translation="MTSLRLLIFLLLLPAALWLTPPAAAQSGSVRGLLDQSLILYRNG
                     SWDQAGNLLMEALDKPASAPHLSRIYLMLSAVHDQLGDTEKSSYYARKLVRHFPESRY
                     RDAAEFALARASYRDQEYVPALAHLLTIIDRNREPGLVETARMTGSRIVSQGLHESGL
                     ERYLGEFQRAGSRNWLLYWLARREYGLGHRSEGELWLQRLLMNGPEARLQRLAQELRT
                     RPAAELVYALRIGVILPLSGYESANGMDFLRGVAMALQDQPQGVELVLKDSESSIQQG
                     IR"
BASE COUNT         1815 a         3192 c         2944 g         1701 t
ORIGIN      
        1 gggaccagat ttcggggaca cgcggcgcca gaggtcaagt ggggaccagg tttcagggga
       61 cacgcggcgc cagaggtcag gtggggacca ggtttttggg ggtaccctgc agaaaaaggg
      121 attgacactc tgcttgaaat tgattacttt tctatttata aatgaattga aggaggcgtt
      181 gtgccggatg acaaagggct gattctgtgg gttgatgacg agatcgatct gctgcggccg
      241 catgttctct ttctcgaaga gaagggctat agcgtcacca cggtgaccaa tgccgaggac
      301 gccctcgagc tggtgcgcgg cgccaacttt gatctcatcc tcctcgacga gatgctcaac
      361 ggcatggacg gcctctccgc tctcaacgag ttcaagaaca tccgtcccgg cctcccggtc
      421 attatggtga ccaaaaacga ggaggagtcg ctgatggaac aggccatcgg cggcaagatc
      481 gacgactatc tcaccaagcc ggtcaatccc agccagatcc tgctggtctg caaaaaaatc
      541 ctgcaaaaac gcaagatcgc cggcgagcgc atttcacgcg actatacctc cgaattcaac
      601 cagatcgcga tgcgcctgat gggcggcatg gagtggcagg actggatcga tgtccacctc
      661 cgcctctgcg aatatgacat cgaactcgac cagcatccgg acctcggcct caagcagatc
      721 ctctacgacc agaagcgcga atgcaacgcc gaattcgggc gctttgtcga aaagaactat
      781 ctcgcctggc tcggcgccgc cggagcccct cccctctcgg tcgatattgt gcccaggtac
      841 gtcctgcccc acgtgcgcgc cggcaggcgc accctgctca tggtcctcga caatctccgc
      901 ctcgaccagt ggctggtggt cgaacccctg ctgcgggaat tcttcaatat ccggcgggag
      961 tactatttct cgatcctgcc cacggccacc ccctatgcgc gcaacgccat cttcagcggc
     1021 ctcttcccga gcgagattga aaaacagctg cccgagctct ggacgcaggg ggacgagaac
     1081 gacgacgtca gctgcaaccg cttcgagagc caactgctcg aggagcagct gcgccgagcc
     1141 ggcgtggagc tcaagcccgg ccccaagtac gccaaggtcc tcgacatgca ggagcaccgc
     1201 cttatcgccc gcaagatcca ggaattcggc gacctcccgc tctcctcgat tgtgatcaat
     1261 ttcgtcgata tcctcgccca ttcacgcagc tcctccgagc tgatcaagga gatgatcccg
     1321 aacgaatcgg ccttccgctc cctgaccaaa agctggttcg agcactccca catgtatctg
     1381 gccctgcagc agctcgccca gagcggcagc accgtcatca tcacctccga ccatggcagc
     1441 atccgcggcc tgcgcggcgc caaggtgatc ggcgaccggg agacctcgac cagcctgcgc
     1501 tacaagtacg gccgcaacat caaggccgac gccaagaacg ccatggtgat cagggacccg
     1561 cggcagtacc ggctgccggt gcgcggggtc aacaccaatt atctcatcgc caaggaagat
     1621 ttctatttcg tctatccgac caactatcat tattacctga actattacgc ggacagcttt
     1681 cagcacggcg gcatctcgat ggaggagatg gtcctgccgg tgatcaccat ggaaccgaaa
     1741 tagccccgga ggcttgtgca ggaatcccgc tatcagctcc tggagagctg gatcacccgg
     1801 aatgcggacg agaccctcgc gcgcggggtt atcatcgctg aaagcctcac ccccggcgag
     1861 atgctggtct tcaccggcaa cctgggctcc ggcaagacca ccctcatcca ggggatctgc
     1921 cgggggctcg gggtcgagga gaccgtgacc agtccgactt ttaccctgat caacgagtat
     1981 cgcggccggc tgccggtcta tcatttcgat ttctaccgtc tacactcggc cgtcgagctg
     2041 accgatctcg gcctggagga gtatctctac ggcgatggga tctgcctggt cgaatggccg
     2101 gagctgatcg cggagtggct gccggtccgg cacatccggc tgcatctgga gcacggcatt
     2161 cacgccgccg ggcgcatcaa cgccctgcag gtggagcagc agcttgagcc ggtgccgatc
     2221 acccctgacg tccgattgat tgaggtctac cgccatgaac ctgctcgcga ttgagagcgc
     2281 caccccggtc tgcgccgccg ctctgacccg cggggagacg ctggtggtgg agtaccgcgc
     2341 ggcgatccgc aacgcccacg gccgcctgct cacccgcgcc atcgaccggc ttctcgctga
     2401 tgccggctgg cagccgggcg atctcgaggc cctggcggtt tcgatcggtc cgggctcttt
     2461 caccgggctg cgcatcggac tggccctggc caaggggatc gccctggccc gggagatccc
     2521 catcgccgcg gtgccgaccc tcgaggtgct ggcggcccag gctccggtcg aaaggggatt
     2581 gatcgccccg ctggtgcgct cccgggcgga cgagtattat gtcgccctct acgaacgcag
     2641 cgatgccggt ctggccgccc tgcaggaggt gcagattctt ccctgggggg agatcccgcg
     2701 ggcagttcca cccgaggcga tgctgatcgg cggtgcaccg gagcagcccc tgccctccgg
     2761 ctaccgctct gcgcccgccg cctccgccct gcccggcgcc ttgaccgtag cccggctggg
     2821 ctggcagcgg ctgcaggagg gccgacgcga gaatccggac ctgctcgagc ccctctatct
     2881 gcaggaattc atcgtcggca agcccaaaca gccggtcttt tccgtggagg ggcatgttcg
     2941 ctgaccattt tctgccgggg gtgcttgaac tcccgaccct ggcgaggccg ctgcactacc
     3001 gcgccatgcg cgaagcggat ctgccggcga tcgccgtcat cgaaaggcag ctcttcagct
     3061 cgccctggag cgaggagctc ttccgcgagg atctggatca ggagtacgcc ctctcgctgg
     3121 tgctgacgga cgcggagtcc attgagggct atatgatcac ctttctggtc ctggacgaga
     3181 tgcacatcgc caatgtcgcc gtagccccag cctaccagcg gcggggcatc gcctttgagg
     3241 tgctcaggcg gctgcttgtc gccggtcgca ggatgcacta ctttctggcc cacctcgagg
     3301 tgcgccagag caacctgggc gcgatcgccc tatacgagcg gctcggtttt gcaaaggtcg
     3361 ggctgcgcaa gaattattac gaaaacgaac acgaggatgc tgtgctgatg agctgcctcc
     3421 tccagagcaa tcccctgctc gccgccgatt aacgaagcgg ctgccggttt tgccaaggag
     3481 cacctatgga gtggttcaaa cgcgtcaccc ccggtctgga ggcccagacc aaaaaggagc
     3541 tgcctgacgg tctctggatc aagtgcgacc gctgcggcga aatcctttac aaaaaggagc
     3601 tcgagcgcaa ccgcatggtc tgcaccggct gcgccttcca cttccgcatg gacagcgggc
     3661 agtacatcgc tctgctcgcc gacgaaggca gctttgagga gttcaacgag aacctcagct
     3721 ccgccgatcc cctccacttc aaggactcga aaaagtacac cgaccggctg gaggaggcga
     3781 tggccaaaac cggcctcaag gaggcggtgc gcaccggcga gtgcaccctt gccggcctcc
     3841 cggttgtgat ggcgatcatg gatttccgct tcatcggcgg cagcatgggc tcggtggtcg
     3901 gggaaaaggt gagccgggcc atcgatcggg ctgtcgagac ccgccggccc ctgatcatcc
     3961 tctcctcctc gggcggggcg cgcatgcagg agggggcgct ttcgctgatg cagatggcca
     4021 agaccgccgc caagctggcc ctgctcgccg aagcgcgcat tcccttcatc tccatcctca
     4081 ccgacccgac cgccggcggg accacagcca gcttttccat gctcggggat gtgatcatcg
     4141 ccgagcccgg ggcctttatc ggcttcgccg gtccgcgcgt catcaagcag accatcggcc
     4201 aggacctccc ggaggggttc cagcgcgccg agttcgtgca ggagcacgga tttgtcgaca
     4261 tgatcgtcga ccgccgccag ctcaaggagc agatcgagcg catcctggtc tggttcgtcg
     4321 gtccggccag gaaaaagccg cggcgggcgg cgccgaagaa aaagagcgcg ccggaggctg
     4381 ccgaaaccgt ggcctccgct ccaaagcgtg gcaaagcagc cggggaaaaa tcagcgaaac
     4441 gcaccgggtc atccgcagcc aaaccacaga ccaggaagaa cagcaccacc aaaccagcga
     4501 gcgaagcgtg agaattcttc tgaccaacga tgacggcatc aacgcccagg ggctcgccgc
     4561 cctggctgcg gagatggcca aacacggtga aatcatcgtc tatgccccgg cctcggagat
     4621 gagcgcggtc ggccatgcca tcaccctcag cgatcccctg cgcttcagcg agtgccggaa
     4681 gaacggcgag ttcttcggca tcgccgtgcg cggcactccc gctgactgcg tcaaggtggc
     4741 gatctgggat ctcggccgcc aggaccgcct gcccgacatc gtcctttcgg gcatcaatca
     4801 gggctccaac accggcatca acaccatcta ttcgggaacg gtgtcggcgg cgaccgaggg
     4861 ggcgatctcg gggatcccgg cggtcgccat cagcctggcc tcctacacca gcggcgattt
     4921 cagcggggcg gcggcctttg cgccgatcat cgcgcgccag ctgctcgccc atccccttcc
     4981 gcccggggtt tttctcaatg tcaatgtgcc ggcgaagccg ctggagcaga tccgcggcgt
     5041 gcgcatcacc cgccagggca tgaccaacta ccgggaggag tacgagatcc gcaaggaccc
     5101 ccacagccgc aattactact ggctgaccgg cgacaagatg gaggtggagc cggatgatga
     5161 gatcgacgac cgggccatca tcgatgacga gatttcggtg acgcccgtcc actgcgacat
     5221 gacctattat cccggactgg atatgctccg gaaatggcag atcagcctct gaacgcaccc
     5281 gtgaaagcgg ccccatggaa catctcgaac ggatcctgat cctcgacttt ggctcacagt
     5341 acacccagct cattgcgcgg cgcgtgcgcg agctcggggt tttttccgaa atccgcccct
     5401 actctatctc tccggccgag ctggcgcaga tccagccgcg ggggatcatc ctctcgggcg
     5461 gcccggcttc ggtctacggc gcggaggccc cactccccga acccgccctc ctcgcccagg
     5521 acatccccct tctcggcatt tgctatggcc tgcaggtgat cggccagctc tgcggcggcg
     5581 aggtcggccg ggcgccccag cgcgaatacg gccgcgccga gctgcagatc gatgaaacgg
     5641 cgacgctctt cgacgggctc ggggatttcc tcaccgtatg gatgagccac ggcgaccatc
     5701 tcacccgcat cccccccgat ttcagcgtca tcgcccatac cgtcaatgcc ccgatcgccg
     5761 ccatccgcca tcgccatcgc cccctctacg gcatccagtt ccatcccgag gtagtgcaca
     5821 ccccgcgcgg cagcgaactg ctcgccaatt tcgtcttcaa ggtctgcggc tgccggggcg
     5881 actggaacgc cagctctttt gtcgaaagga cggtggcgga gatccgcgag cgggtcggcg
     5941 gggagcaggt gctttgcgcc ctctcaggcg gggtcgattc aaccgtggcc gccgcgctgg
     6001 tgggccgcgc cgtcggccgc cagctcacct gcatctttgt tgacaccggc ctgctgcgcg
     6061 aaaacgaggc cgcggaggtt tgcagttttt accgcaacgc cgattttgaa ttcaggttcg
     6121 tcgatgccgg cgagcgcttc ctctcccgcc tggcgggggt gaccgatccc gagcaaaagc
     6181 gcaagatcat cggcagggag ttcatcgctg tctttgagga ggaggcggcg cgcctgggcg
     6241 gagtgggcta tctggtgcag ggcaccctct accccgacgt catcgagagc gtctcgacca
     6301 gggggccctc cgccaccatc aagagccacc acaacgtcgg cgggctgccg gaaaagatgc
     6361 gcctcaagct catcgagccc ctgcgcgagc tcttcaagga tgaggtgcgc agggcgggcc
     6421 gcgagctgca ggtcccccct ttcattctcg gccgccatcc ctttcctgga cccggtctgg
     6481 ctgtgcgcat cctcggcgag atcacccctg agcgcctggc catcctcaaa ccggccgacc
     6541 gcatcttcat ggaggagctg cgccggagcg gggaatatga ccggatctgg caggccttcg
     6601 ccgtgctgct gccggtccag accgtggggg tgatgggcga cgagcgcacc tatgagaatg
     6661 tcatcgccct gcgcgccgtg accagcatgg acggcatgac tgccgactgg gcggagatcc
     6721 cgcgctccct gctggcgctg atttccaacc gcattatcaa cgaggtcaag ggcgtcaacc
     6781 gggtggtcta cgacatcagc tcgaaaccgc ccagcaccat cgaatgggaa tagcggaggg
     6841 cgctgccctt acgcgttcga ccggtctggc gaggatgctg ccagggggaa gatatagata
     6901 ataaagggga taaggaggcc gctaatgtgc gggatagtgg gctatatcgg cagtcaaaat
     6961 gcgctgcccc tgctgctcaa cgggctgcac cggctggagt accgcggcta tgacagcgcc
     7021 ggggttgcgc tctggaccga tgaggggatc tggatgcaaa aggcggccgg caagatcgcc
     7081 gagctggaac ggatattgcc agcggagccg ccgcagagta ctctgggaat cggccatacc
     7141 cgttgggcca cccacggcga gcccaattcg gtcaatgccc atccccactg cgactgcggc
     7201 cgtcgcatcg cggtggtgca caacggcatc attgagaatt ttcccctcct gcgcgatgag
     7261 ctcatccgca aaggccatac cttccaatcc cagaccgaca gcgaggtgct ctgccatctc
     7321 ttcgaggagt atctcgaacg gggcatggag ctgggcgagg cggtccgtca ggggctcgcg
     7381 tgcgtacagg gcacctacgg cctggctatc atcgaccggg atcatcccga taccctggtg
     7441 gccgcacgca acggctcgcc cctggtgatc ggccacgccg aggaggagtt tttcctcgcc
     7501 tccgatgccg cccctctggt gggggtgatc aagagcgtca cctatctgga tgacggcgag
     7561 ctggcggtgc tcacgcgcag cggcatgacg gtacgcacca tcgagaactg cccggtcgac
     7621 aagcactccg aacccatcga tctcgatctt cagcagatcg aaaagtcggg ttacgcccat
     7681 ttcatgctca aggagatcat ggagcagccc agcaccctcg ccgacaccct gcgcgggcgg
     7741 ctgatgtggg aggaggggac tgcccggctc gggggcatcg agcatgattt cgaatccctg
     7801 ctctacgccc gccgcatcgt cctgaccgcc tgcggcacct cctggcactc ggccctgatc
     7861 ggcgagttca tgttcgagga gatgctcggg atccccgtcg aggtggagta cgcttccgaa
     7921 ttccgctatc gcaatcccat cgtcaacgac gattccgtgg tcatcgccat cagccagtcc
     7981 ggggagacgg ccgacaccct ggccgcgatc cgcgaggcca aacgcaagcg cagcaaggtt
     8041 tacggcatct gcaatgtggt gggctcgagc atcgcccggg agaccgatgc gggggtttat
     8101 cttcacgccg gccccgaaat cggggtggcc tccaccaagg ccttcacctc gcaggtgacg
     8161 gtcctcgccc tcatcaccct gctgctgggg cggatgcgct cgatctcatc ggcgcgcggg
     8221 cgcgagctgg tcagcgagct gcaggcgatt ccaggcaaga tccgcaccat ccttgagcag
     8281 cacgaagaga tccgccagat cgccgagatc caccagtatg tgcgcaactg cctctacctg
     8341 gggcgggggt acaactatcc ggtcgccctt gagggggcgc tcaagctcaa ggagatcgcc
     8401 tacgtccatg ccgagggtta tccggccgcg gagatcaagc acggccccat cgccctgatc
     8461 gacaaccaga tgcccgtggt ggtcatcgcc acccgcgact cgatctatga aaagatcctc
     8521 agcaacatcg aggaggtgcg ggcgcgcagc ggccgggtca tcgccatcgc caccgagggc
     8581 gaccaggaaa taaaaaaaca cgctcaatat gttatttatg taccacagac tatcgaaatg
     8641 ctttcaccct tgctcacaat catccccttg cagctgttcg catactatat ggctatcctg
     8701 cgcaactgcg atgtggatca gccgcgaaat cttgcaaaaa gcgtcaccgt tgaatagtat
     8761 ttacgcgaag gagtacacct tgcccctcaa gatccggtcc gctggtgcgc accctggctt
     8821 gacatccctc cgtttgctca tctttctgct cctgcttccg gcggccctgt ggctgactcc
     8881 gccggccgcc gcccagagcg gcagcgtcag gggcctgctc gaccagagcc ttatcctcta
     8941 ccggaatggc tcctgggacc aggccgggaa tttgctgatg gaggccctgg acaagcccgc
     9001 gagcgctccg cacctcagca gaatctacct catgctcagc gccgtccacg accaattggg
     9061 cgataccgaa aagtcatctt attacgcccg caagctggtc cgccattttc cggagagccg
     9121 gtaccgcgac gctgccgaat tcgcccttgc gcgcgccagc taccgcgacc aggaatatgt
     9181 gcccgccctg gcccatctcc tcaccatcat cgaccggaac cgggagcccg ggctggttga
     9241 aaccgccagg atgaccggca gccgcatcgt ctcccagggg ctgcacgaaa gcggcctgga
     9301 gaggtatctg ggcgaatttc agcgcgccgg cagccgcaac tggctcctct actggctggc
     9361 caggcgggag tacggcctcg gccatcgcag cgagggggag ctctggctgc agcggctgct
     9421 gatgaatggc ccggaagcgc ggctgcagcg cctggcgcag gaactgcgca cccggcccgc
     9481 cgcagagctc gtttacgccc tccgcatcgg cgtcatcctg cccctgagcg gctacgaatc
     9541 ggcgaatggt atggactttc tccgcggcgt ggccatggcc ctgcaggatc agccccaggg
     9601 ggtggaactg gtgctcaagg attctgagag cagcatccag caaggcatcc gg
//
LOCUS       DAOPWU010000110         9648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7110_length_9648_cov_4.897321, whole genome shotgun sequence.
ACCESSION   DAOPWU010000110 DAOPWU010000000
VERSION     DAOPWU010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7110_length_9648_cov_4.897321"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKM23_07305"
     CDS             complement(<1..361)
                     /locus_tag="PKM23_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNY91320.1"
                     /translation="MMLMLSIVTTYLLPFIVVLGVLIIAHEAGHFLAAKAFGIRVERF
                     SVGFPPRLFGKQIGETDYCVSAVPLGGYVKLSGMIDETMDTEGVKGEPWEFMSKPVWV
                     RMLVLFAGPLANVLLIIF"
     gene            complement(480..1646)
                     /locus_tag="PKM23_07310"
     CDS             complement(480..1646)
                     /locus_tag="PKM23_07310"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNY91321.1"
                     /translation="MASKKIIILGSTGSIGLNALHVVEQMPERFRVLALTTHRQIDHL
                     LQLAQRHRPALAVVTGAEPDGEQRAAFRALGVELRTGAEALPALCAELDYDLLVNAVV
                     GAVGFTPTLTAVERGRSVALANKEAMVIGGALVTAAAAASGAEILPIDSEHSAIWQCL
                     MGEEKTAVEEIILTGSGGPFRTWPAEDLVHVTVEQALKHPNWNMGRKITIDSATMMNK
                     GLEIIEACWLFGLTEEKIRVVIHPQSIIHSMVAFRDGSIKAQLGLPDMRVPIQLAMTW
                     PERCASAFPRLDFSTLDRLTFAPPDEAKFPALALARAAIRTGGSAPAVLNAANEAAVN
                     LFLEERIGFLAIARLIDGALQAHSIDSVPSAGALLEADHWARDHVTEAAAHLRR"
     gene            complement(1639..2472)
                     /locus_tag="PKM23_07315"
     CDS             complement(1639..2472)
                     /locus_tag="PKM23_07315"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNY91322.1"
                     /translation="MEYWRFIDSGLADGFSNMAEDAALARCWQGKPVLRLYGWRPPAI
                     SLGFHQRLEDLDLERCSRDNIDVVYRPTGGRAVLHDDEVTYAVILGPGSRLYDDRVMP
                     VYERISAGIMAALALLRIPLAFERAPARKGTGGKNALSSLCFASSIQHEIGHGGKKMI
                     GSAQRRFGATVLQHGSILLGGAHLRLVDYIKPQDQDGGEGEWRGRARRFMQEKTVALN
                     EISPQPLDYTAVAAALRQGFAERWGIAWSDSELTSEEKNLATELRGEFRDKTTARGQI
                     G"
     gene            complement(2496..3269)
                     /gene="lpxA"
                     /locus_tag="PKM23_07320"
     CDS             complement(2496..3269)
                     /gene="lpxA"
                     /locus_tag="PKM23_07320"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNY91323.1"
                     /translation="MIEIHPTALVDNRAEIGDNVQIGPFAIIEEDVVIGAGTTIGPHV
                     LAANGARIGKNCRIHNGAVVATLPQDLKFGGEKSLFEIGDNTTVREFATLNRGTAAHG
                     KSSIGSNCLLMAYTHVAHDCTVGDNVIMANGVQLGGHVTIEDWAIIGGMTPVHQFCHV
                     GQHCMIGGGFRVIQDVPPYILASEEPLRFAGLNSIGLRRRGFSSETLMLLKRVYRILY
                     RSELNVSQAVVRIKSEFEITPEIGSVLRFIETSDRGIIH"
     gene            complement(3310..4725)
                     /locus_tag="PKM23_07325"
     CDS             complement(3310..4725)
                     /locus_tag="PKM23_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNY91324.1"
                     /translation="MIKNQRTIIHPVSYSGVGLHTGNKTKITFVPAPANTGILFRRVD
                     MADSPVIPADVDHVLDISRGTTIGIGEVKVHTVEHVLAAVSGLEIDNLICEVDGNEPP
                     VGDGSSLPFVEILLKAGFEEQDSPRDYLIIDKTITYHDPSRGIDIVVFPSDEFRITFM
                     VDYKNPALGTQYTSMYSLHEEFATEFAAARTFCFLSEVEQLRKANLIRGGALDNAVVI
                     IDRDMSEDELGGLRQLFEIDEKVTLSTNGILNGKTLRYPNEPVRHKALDLIGDLALLG
                     MPLKAHVMAARSGHQANAELVKLIRKEYKKKLIRSKFKSDNKGVFLDQEAILKILPHR
                     YPFLLVDRIIDLVPEEHVVGIKNVSNNEPFFQGHFPGKPIMPGVLIIEAMAQVGGILL
                     LNAEVDPATKLVYFTGIDNVRFRKTVTPGDTLRFEVELTAARRSMCKMMGRAFVDDEL
                     VCEAELMAAIVDRKSEEPAVM"
     gene            complement(4753..5805)
                     /gene="lpxD"
                     /locus_tag="PKM23_07330"
     CDS             complement(4753..5805)
                     /gene="lpxD"
                     /locus_tag="PKM23_07330"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNY91325.1"
                     /translation="MAFSLAEIASWINAVVDGDETLEITGLAKIEEARPGELTFIANP
                     KYEKYIADTAASAVIVANNFSGSSKTLLRCADPYYAFMLLVERLYGGPPELPAGIHPS
                     AVIAEGCRIDPGSAVGPLVVIGRGSTIGPGVQLHPGVVLGEEVQVGAGSILYANVSVR
                     ERCRIGERVIIHCGAVVGSDGFGFAFKEGRYHKIPQTGIVIIEDDVEIGANTTIDRAT
                     LGATLIRAGAKLDNLIQVAHNVEIGSHTAIAAQAGISGSSRVGGYVRMGGQVGISGHL
                     HIGDNVVLGGQAGVTKDIPAGLFYSGYPARPHMQAMREEAALARLPELLKRIRQMEKE
                     IESLNRKLNADADEPR"
     gene            complement(6076..6621)
                     /locus_tag="PKM23_07335"
     CDS             complement(6076..6621)
                     /locus_tag="PKM23_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNY91326.1"
                     /translation="MKWVVLAVVMVALTAGSASAQKLAYINSQKIIATYKEAQDAQDR
                     LNKISAGWEEEAKLMQKQFQELGEQLESQSLLLSEERKAEKQQELQNLYMKIQQYQAE
                     KWGQSGELYKKQEEMMQPIFDKINAAIKKISEEQSYDFVFDSINGNIVYASPRQVDLT
                     DEVLAELEKGLPAKTTPKTPR"
     gene            complement(6680..8998)
                     /gene="bamA"
                     /locus_tag="PKM23_07340"
     CDS             complement(6680..8998)
                     /gene="bamA"
                     /locus_tag="PKM23_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNY91327.1"
                     /translation="MTLLQRRYFIPLIALLLILPLAGIAQKRSVKILGLSVEGNKTVD
                     ASMIRLSSGLNIGSEVTGDGVQEAIRQLWRLSLFSDVQILVDRELADGAYLTIHVEEH
                     PRLEKVEIEGNKKLKKDDIESILNFYPGQVVSPTLISRAKSKLYDKYKEKGYLLAKIK
                     ANQYVSRTDTSRVLVRFVFDEGSKVQVEKINFHGNTRFSDKKLRKQLKGTKEDRWWRG
                     ADFDKVKYTEDQGKLIDFYLNHGYRDAEILRDSLYYSPDQSDMFVELWIHEGEQYTFG
                     DISWKGNTVFPTELLAANLGFAKGEVYSKKKLDEALQKRIGTLYYDRGYIYSQVEPTE
                     VPGDSNVLNIDFSVSEGKQARINEIHITGNTRTKEKVIRRELKILPGDTFSRTLLERS
                     QREVWMLNYFGNVEPNVKPIDEEKVDLEFKVEEKSTDTANMSAGYSEVDKFIGSIGLA
                     MANLFGNGQRLSTDWNFGRYYRSFNVSFTEPWLRDTPTLAGVSLYDTKRDAAYYGYKQ
                     ESRGFSLRLGRDLSWPDNFFRGDWIYGLDETSLSDFNSYYVAANPNGIVTQKWPLTSS
                     SITQIFTRNSLDRPEFPTSGSEVTLTNELSGTFLGGNVDFHKHTLRMDWFMPAYWKFV
                     LYSSFQAGYMETIGDNRSIPYLEYFFMGGDGLSRSIPLRGYDDPLGESTSYIEAGGKS
                     YLKYTAELRIPIAPNPTIFALIFGEAGKVWPDFRHADPFTMKRSVGIGGRVYMPMVGI
                     IGFDYGYGFDRLDANGKSDPKWKMHFVFGRSF"
     gene            complement(9104..>9648)
                     /locus_tag="PKM23_07345"
     CDS             complement(9104..>9648)
                     /locus_tag="PKM23_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNY91328.1"
                     /translation="RWDQIRFKLSSDNPNLGTTEAQLLASLGYSERTLQTKAMDVIGI
                     NTDNYLLRPFFKPVERTIEHFLQLDYVRFSSRFTRNFLDANLNNNLQVNSRLALLRSS
                     RLILGKYLSRNVFLQYTGQVEAGVEYRYQSRGLGLRHSLGLEYRISPQVLLELEYNYD
                     SLMLYNRDDKRIMVRHWFPF"
BASE COUNT         1770 a         2663 c         3183 g         2032 t
ORIGIN      
        1 caaagatgat gagcagcaca ttggccagtg ggccggcaaa aagcaccagc atccgcaccc
       61 agaccggctt ggacatgaac tcccatggct cccctttgac cccttcggta tccatggttt
      121 cgtcgatcat gcccgagagc ttgacgtagc cgccgagcgg cacagccgag acacaatagt
      181 cggtctcgcc gatctgtttg ccgaagaggc gtggcggaaa gccgacggaa aaccgctcca
      241 cgcggatgcc aaaggccttg gccgcgagaa aatggcccgc ctcgtgggca ataatgagga
      301 cgccgaggac cacgatgaag gggagcaggt aggtggtcac aatcgaaagc atgagcatca
      361 tgatgatttc ctttgttctt tatattgggt actacggtta attcatttta tggttacagc
      421 gagtttcatt caggcggatt tacagccgcc gcaaggcttt ccgtggcgga ccaaccgcat
      481 caccggcgca ggtgcgctgc cgcctctgtg acatgatcgc gggcccagtg gtcggcctcc
      541 agaagggcgc cggccgaagg aacgctgtcg atgctgtggg cctgcagggc cccgtcgatg
      601 agccgggcga tagcgagaaa gccgatgcgc tcctcgagaa agaggttgac cgccgcttcg
      661 ttggcggcgt tgagtacggc cggggcgctt ccgcccgtgc ggatagcggc gcgcgccagg
      721 gcgagggccg gaaatttggc ctcatccggc ggggcaaagg tgagccggtc gagggtgctg
      781 aaatcgaggc gcggaaacgc ggaggcgcag cgctccggcc aggtcatcgc cagctggatc
      841 ggcacccgca tgtcgggcag gccgagctgg gccttgatcg agccatcccg aaaggccacc
      901 atcgaatgga tgatggactg gggatggatg accaccctga tcttctcctc cgtgaggccg
      961 aaaagccagc aggcctcgat gatctccagt cccttgttca tcatcgtggc cgaatcgatg
     1021 gtgattttgc gtcccatatt ccagttggga tgcttgaggg cctgctcgac ggtgacgtgc
     1081 accagatctt cggcgggcca ggtgcggaag gggccgcccg agccggtgag gatgatctcc
     1141 tcaacggccg ttttctcctc gcccatcagg cactgccaga tggcgctgtg ctcgctgtcg
     1201 atgggaagga tctccgcgcc gctcgccgcc gcagcggcgg tgaccagggc cccgccgatg
     1261 accatcgcct ccttgttggc gagggcgacg ctgcggccgc gctcgaccgc ggtcagggtg
     1321 ggggtaaagc cgacggcgcc gacgaccgcg ttgacgagca gatcgtaatc cagctccgcg
     1381 caaagggcgg gcagggcctc cgcgccggtg cggagttcga cgcccagggc gcggaaggcg
     1441 gcgcgctgct ccccgtccgg ttcggcgccg gtgacgacgg cgagggcggg gcggtggcgc
     1501 tgcgccagct gcagcagatg gtcgatctgg cggtgggttg tcagggccag aaccctgaag
     1561 cgctccggca tctgctcgac cacatggagg gcgttgaggc cgatcgaacc ggtgctgccc
     1621 aggatgatga tctttttgct agccaatctg ccctcttgcg gtggttttgt ccctgaactc
     1681 cccgcgcagc tccgtggcga ggtttttctc ctcgctggtc agctctgaat cgctccaggc
     1741 gatcccccag cgctcagcga aaccctgcct cagggcggcg gccaccgcgg tgtaatcgag
     1801 tggctgcggg ctgatctcgt tgagggccac agtcttttcc tgcataaagc gccgggcgcg
     1861 gccgcgccat tccccctctc ctccatcctg atcctgcggt tttatatagt cgaccaggcg
     1921 cagatgagcc ccgccgagca ggatcgaacc gtgctgcagc acagtggcgc cgaagcggcg
     1981 ctgggcgctg ccgatcatct ttttgccccc gtggccgatt tcatgctgga tcgaagaggc
     2041 gaagcagagg ctggaaaggg cgtttttgcc gcccgtcccc ttgcgggccg gggcgcgttc
     2101 aaaggcgagg ggaatgcgca gcagggccag tgcggccatg atgcctgcac tgatgcgttc
     2161 atagacgggc atcacccggt catcgtagag ccggctgccg ggcccgagga tgacggcata
     2221 ggtcacctca tcgtcgtgga gcacggcgcg tcccccggtg gggcgataga cgacatcgat
     2281 gttgtcacgg ctgcagcgct cgaggtcgag atcttccagg cgttgatgaa agccgagcga
     2341 aatcgccggt ggacgccagc cgtagaggcg caggaccggt ttcccctgcc aacagcgcgc
     2401 cagggcggca tcttcggcca tattggaaaa gccgtccgca agcccgctgt caatgaaacg
     2461 ccaatactcc atcccctcac ttcctgcagc tcgactcaat ggatgatgcc gcgatcggag
     2521 gtttcgatga agcgcaggac gctgccgatc tccggggtga tctcgaactc gctcttgatg
     2581 cggacaaccg cctggctgac gttgagctcc gagcggtaga ggatgcggta gacgcgcttg
     2641 aggagcatca gggtctcgct gctgaagccc cggcggcgca gaccgatgga gttgaggccg
     2701 gcgaagcgga ggggctcctc ggaggcgagg atgtagggcg gaacgtcctg gatgacccgg
     2761 aagcccccgc cgatcatgca gtgctggccg acgtggcaga actggtggac gggagtcatg
     2821 ccaccgatga tggcccaatc ctcgatggtg acatggccgc cgagctgtac accgttggcc
     2881 atgatgacgt tgtcgccgac cgtgcagtcg tgggcgacgt gggtgtaggc catgagcagg
     2941 cagttgctgc cgatggagct tttgccgtgg gcggcggtgc cgcggttaag ggtggcgaat
     3001 tcgcgcactg tggtattgtc gccgatctcg aaaaggctct tctcgccgcc gaacttgagg
     3061 tcctggggca gggtggcaac cacggccccg ttgtggatgc ggcagttctt gccgatgcgg
     3121 gcgccgttgg cggccaaaac atgcgggccg atggtcgtcc ccgcgccgat aaccacgtct
     3181 tcctcgatga tcgcgaaagg gccgatctgc acgttatcgc cgatctcggc gcgattgtcg
     3241 accagggctg tgggatggat ctcgatcaag ctggttctcc tttgtacggg gatggatttc
     3301 cggcagcggt cacatcaccg ccggttcttc gctcttgcgg tcgacgatgg cggccatgag
     3361 ttcggcttcg cagaccagtt cgtcatcgac aaaggcccgc cccatcatct tgcacatcga
     3421 acggcgcgcg gcagtcagct ccacctcgaa gcgcagggta tcccccgggg tgacggtctt
     3481 gcggaagcgg acattgtcga tgccggtgaa atagaccagc ttggtggccg gatcgacctc
     3541 ggcattaagg agcaggatgc ccccgacctg ggccatggcc tcgatgatga gaaccccggg
     3601 catgatcggc ttgccgggaa aatggccctg gaaaaagggc tcgttgttgc tgacattctt
     3661 gatgcccacc acatgctcct cggggaccaa atcgatgatg cggtcgacga gcaggaaggg
     3721 atagcggtgg ggcaggatct tgaggatggc ctcctggtcc agaaagaccc ccttgttgtc
     3781 gctcttgaat ttggagcgga tcagcttctt tttatactcc ttgcggatga gcttgaccag
     3841 ttcggcgtta gcctggtggc ccgagcgcgc ggccatgacg tgagccttga ggggcatgcc
     3901 gagcagggcg agatcgccga tgaggtcaag ggccttgtgg cggaccggtt cgttggggta
     3961 gcgcagggtt ttgccgttga ggatgccatt ggtcgagagg gtcacctttt cgtcgatctc
     4021 gaagagttgg cgcagcccgc cgagttcgtc ctcgctcatg tcgcggtcga tgatgacgac
     4081 ggcgttgtcg agggcgccgc cgcggatgag gttggccttg cgcagctgct cgacctcgct
     4141 gaggaagcag aaagtgcgcg cggcggcgaa ctcggtggcg aactcttcat gcagggagta
     4201 catgctggta tactgggtgc cgagggccgg attcttgtaa tcgaccatga aggtgatgcg
     4261 gaactcgtcg gagggaaaga cgacgatatc gatcccgcgc gaggggtcgt gataggtgat
     4321 ggtcttgtcg atgatgagat agtcgcgcgg cgaatcctgc tcctcaaaac cggccttgag
     4381 gaggatttcg acaaagggca gggagctgcc gtcgccgacg gggggctcat tgccgtcgac
     4441 ctcgcagatg aggttgtcga tctcgaggcc gctgacggcg gcaagcacat gttcaacggt
     4501 atggaccttg acctcgccga tgccgatggt ggtgccgcgg gagatatcga ggacgtgatc
     4561 gacgtcggcg gggatgaccg ggctgtcggc catatcgacg cggcggaaga ggatgccggt
     4621 attggccggg gccgggacga aggtgatttt ggttttattg ccggtatgga gccccacacc
     4681 ggagtaggag accgggtgta ttatcgtgcg ctggttcttg atcattcatc ctccagggtt
     4741 ccgcgccctc attcagcgcg gttcatccgc gtccgcgttc agcttgcggt tgagcgattc
     4801 tatttccttt tccatttgcc gtatccgttt gagcagctcg ggcagacggg ccagagcggc
     4861 ctcttcgcgc atggcctgca tgtgcggccg ggcgggatag ccggaataaa agagtcctgc
     4921 ggggatatcc ttggtcacac cggcctggcc gccaaggacg acgttgtcgc cgatgtggag
     4981 atgacccgaa atgccgacct gtccccccat gcggacgtag ccgcccaccc gcgagctgcc
     5041 cgagatcccc gcctgggcgg cgatggcggt gtggctgccg atctcgacat tatgggcgac
     5101 ctggatcaga ttatcgagct tggccccggc gcggatgagg gtggcgccga gggtggcgcg
     5161 atcgatggtg gtgttggcgc caatctcgac gtcgtcttcg atgatgacga tgccggtctg
     5221 ggggatcttg tgataacgcc cctccttgaa ggcaaagccg aagccgtccg agccgacgac
     5281 cgcaccgcag tggatgatca cgcgctcgcc gatgcggcag cgctcgcgca ccgagacgtt
     5341 ggcatagagt atggagccgg cgccgacctg cacctcctca ccgaggacga caccgggatg
     5401 gagctggacc ccgggtccga tggtggagcc gcgtccgatg acgacgaggg ggccgaccgc
     5461 gctgccgggg tcgatccggc acccctccgc gatcaccgcg ctgggatgga tgccggcagg
     5521 cagctccggc ggaccgccgt agaggcgttc gaccaggagc atgaaggcat agtagggatc
     5581 tgcgcagcgc agcagggttt tgctgctgcc ggaaaagttg ttggcgacga tgaccgccga
     5641 agcggcggtg tcggcgatat atttttcata tttgggattg gcgataaagg tcagttcgcc
     5701 gggccgggcc tcttcaatct tggcgaggcc ggtgatctcc agtgtctcat cgccatcgac
     5761 aacggcattg atccaggatg cgatctctgc aaggctgaag gccatcaaat ttcctcagaa
     5821 aggctcgtgt gtgcatgata acaaccggtc atggcgtcct attcctgcgc gccgggcggc
     5881 cgtgcggggc gcgtcaggct gccggatcag ggcgtcctat aaatgccaaa cacggaacag
     5941 cccaggcgcc gccggttttg ataaaccggc gctggtcccg gctctggccg ggaccagctt
     6001 ccttgcgata tccgtgtcct gccgctcccg atgggtcatc ggggctatat ttctccggtt
     6061 gggccggatg aaggatcagc gcggtgtctt gggggtcgtc ttggccggca gtcccttttc
     6121 gagttcggcg aggacctcat cggtcaggtc gacctggcgg gggctggcat agacgatgtt
     6181 gccgttgatg ctgtcaaaga caaaatcgta gctctgctct tcggagatct ttttgatggc
     6241 cgcattgatc ttgtcgaaaa tgggctgcat catctcttcc tgcttcttgt agagctcgcc
     6301 gctctggccc catttttcag cctgatactg ctggatcttc atgtagagat tctgcagctc
     6361 ctgctgtttc tcggccttgc gctcttcgct gagcagcagg gactgggact cgagctgttc
     6421 gccaagttcc tggaactgct tctgcatgag tttggcctct tcttcccaac cggcgctgat
     6481 cttgttcagg cgatcctggg cgtcctgggc ttccttgtag gtggcgatga ttttctggga
     6541 gttgatgtag gccagctttt gcgcggaagc agagccggcg gtcagggcga ccataaccac
     6601 agccagaaca acccacttca tttttttcac gattacctcc tgggtattgg gataaggaat
     6661 gattgctttg tttttctggt cagaagctgc gtccaaagac gaaatgcatc ttccatttgg
     6721 gatcggattt gccgttcgca tcgaggcggt caaagccgta gccgtaatcg aaaccgatga
     6781 tgccgaccat gggcatatag acgcggccgc cgatacccac tgagcgcttc atggtgaagg
     6841 ggtcggcgtg gcggaaatca ggccagacct tgcccgcttc gccgaaaatg agcgcgaaga
     6901 tggtgggatt cggggcgatc gggatgcgca gttcagcagt gtatttcaga taggatttgc
     6961 ctcctgcttc aatataagag gtgctctcac cgagcggatc gtcatatccg cgcaggggta
     7021 tcgaacggga gaggccgtcg ccccccataa aaaagtattc gagatagggg atgctgcggt
     7081 tgtcgccgat ggtctccata tagccggcct ggaagctgga atagaggaca aatttccagt
     7141 aggcgggcat gaaccagtcc atgcgcaggg tatgtttgtg gaaatcgaca ttgccgccga
     7201 gaaaggtccc gctcagctcg ttggtcaaag tcacctccga tcccgaggtc gggaattcgg
     7261 ggcggtcgag gctgttgcgg gtgaagatct gggtgatgct gctgctggtg agcggccatt
     7321 tctgggtcac aatcccgttg gggttggcgg cgacgtaata gctgttgaaa tcggagaggg
     7381 aggtttcgtc gaggccatag atccagtcgc cgcggaaaaa gttgtccggc cagctcaggt
     7441 cgcggccgag gcgcagcgaa aagccccggc tctcctgctt gtagccataa taggcggcat
     7501 cgcgtttggt gtcgtagagg gagaccccgg ccagggtcgg ggtgtcgcgc agccagggtt
     7561 cggtgaagct gacgttgaac gagcggtaat agcgcccgaa gttccagtcg gtgctgaggc
     7621 gctggccgtt gccgaagagg ttggccatgg ccaggccgat cgagccgatg aacttgtcga
     7681 cttcagaata gccggccgac atgttggcgg tgtcggtcga tttctcctcg accttgaatt
     7741 cgaggtcgac cttctcctcg tcgatgggtt tgacgttggg ctcgacgtta ccgaaatagt
     7801 tgagcatcca gacctcgcgc tggctgcgtt cgagcagggt gcggctgaag gtgtcgcccg
     7861 ggaggatctt gagctcgcgg cggatcactt tttccttggt gcgggtgttg ccggtgatgt
     7921 ggatctcgtt gatgcgggcc tgtttgcctt cgctgaccga aaaatcgata ttgagcacgt
     7981 tgctgtcgcc cggaacctcg gtgggctcga cctgagagta gatatagccg cggtcataat
     8041 agagggtgcc gatgcgcttt tgcagggctt cgtccagctt tttcttgctg tagacctcgc
     8101 ccttggcaaa gccgagattg gcggccagaa gctcggtcgg aaagacggtg ttgcccttcc
     8161 aggagatgtc gccgaaggtg tactgctccc cctcgtgaat ccagagctcg acgaacatgt
     8221 cactctggtc ggggctgtaa tagagggaat cgcggaggat ttcagcatcg cggtagccgt
     8281 gattgagata gaaatcgatg agcttgccct gatcctcggt gtatttcacc ttgtcgaaat
     8341 cggcgccgcg ccaccagcgg tcctctttgg tccccttgag ctgcttgcgc agttttttgt
     8401 cggagaagcg ggtattgcca tggaaattta ttttttcgac ctggaccttg ctgccctcgt
     8461 cgaagacaaa gcgcacgagc acgcggctgg tgtcggtacg gctgacgtac tggttggcct
     8521 tgattttggc gagcagatag cccttttcct tgtacttgtc atagagcttg ctcttggcgc
     8581 ggctgatcag ggtggggctc accacctggc cgggatagaa attgagaatg ctctcgatat
     8641 cgtccttctt gagctttttg ttgccctcga tctccacctt ctcgaggcgc gggtgctcct
     8701 caacatggat ggtgagatag gcgccgtcgg ccaattcgcg gtcgacgaga atctgcacat
     8761 cggaaaagag cgagagacgc cagagctggc ggatagcttc ctgaacgccg tcaccggtca
     8821 cctcgctgcc gatgttcaat ccggaactga ggcggatcat gctggcatcg acggtcttgt
     8881 tgccctcaac ggagagacca agaatcttga ccgaccgctt ctgggcgatg ccggccagag
     8941 gcaaaatcag caacagggcg atgagcggga tgaagtaccg gcgctgcagt agggtcatga
     9001 aggatcagcc ttatgttcgg ttcaattaaa actttttaat ttaatgaatt tttgcattct
     9061 tttaaagaga aatatttttt ttgaatgcgt tttgaccctg ctttcagaac ggaaaccagt
     9121 gacggaccat tatccgtttg tcatccctgt tgtacagcat caaactatca tagttgtatt
     9181 caagttccaa taaaacttgc ggactgatgc ggtattccag tccgaggctg tggcgcaggc
     9241 cgagccccct ggactggtaa cggtactcca ccccggcctc gacctgcccg gtgtactgca
     9301 ggaagacatt gcggctgagg tatttaccaa ggatgagccg gctgctgcgc agcagggcga
     9361 gccggctgtt gacctgcagg ttgttgttga ggttggcgtc aagaaagttg cgggtgaagc
     9421 gcgaactgaa gcgcacataa tcgagctgga gaaaatgttc gatagtgcgt tcgaccggct
     9481 tgaaaaaggg gcggagcaga taattgtcgg tgttgatgcc gatgacgtcc atcgccttgg
     9541 tctgaagcgt ccgttcggaa tagccgagcg atgcgagcag ctgcgcctca gtggtgccca
     9601 ggttgggatt gtcgctgctg agtttgaagc ggatctggtc ccagcggc
//
LOCUS       DAOPWU010000111         9550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7199_length_9550_cov_4.785677, whole genome shotgun sequence.
ACCESSION   DAOPWU010000111 DAOPWU010000000
VERSION     DAOPWU010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7199_length_9550_cov_4.785677"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..347)
                     /locus_tag="PKM23_07350"
                     /pseudo
     CDS             complement(169..347)
                     /locus_tag="PKM23_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010647227.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            775..1437
                     /locus_tag="PKM23_07355"
     CDS             775..1437
                     /locus_tag="PKM23_07355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNY91329.1"
                     /translation="MLFDLDGTLMDTDDQMTERVGRWLSRLHLPHAERLARRMVMLFE
                     SPVNGLVTLLDRIGLDKPLLDLLQRLRHGQKMHRHDFHIIPGVAELLASLRGRYRLGV
                     VTTRSETEAEAFIAQHQLQDIFEFVIGRTSTRRLKPHPEPVRLASLQLDIPVERCLMV
                     GDTTMDIRSAVAAGAPAAGVLCGYGTRKELEQAGATVVVEHITDLAALLNAPVFQRGQ
                     PS"
     gene            1551..2795
                     /locus_tag="PKM23_07360"
     CDS             1551..2795
                     /locus_tag="PKM23_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNY91330.1"
                     /translation="MNSHARVRAALEHRNPDKIPIDFGGHRSSGISAMAYARLKRALG
                     ITSGDIYVYDMMQQLAIIEEPVLTALEVDTVELGRAFWHEERFWKEWTLPDGTPCKVP
                     TSANLARRGEEWYLLAPDSTPVGVQKPGCDFFEQILYPLAESDFEHTDFSDIGAASAH
                     SMRAAAPVPGSHLGMDDKGLAELSEGARRLRASTDRAIQASFGGKMIEGPQALYGMEN
                     YLLYTALYPEQIIRLSEVLCEGYCKSLRKWLPAVAPYIDLIGFGDDLGTNNGPMISPE
                     KYRLYYKPYHARMWRLVKELAPHVKILLHCCGGIEPLLEDLIEAGLDAVNPVQISARG
                     MQLETLKSKYGGRITFWGGGCDTQTILPQGTPDQVRDHVRGQIEIMKGEGGFVFQQVH
                     NIIGRVPIENIMAMFETVIRFR"
     gene            2969..5470
                     /locus_tag="PKM23_07365"
     CDS             2969..5470
                     /locus_tag="PKM23_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNY91331.1"
                     /translation="MKKIWIILAVILLILILFVALAPAFFSEGVGRIVVNQAEKKLNA
                     HIGFNGLSLSLIKNFPRTTLSIDSLQIIPLEPFAKDTLLQSERITATVDLLSYFRNKR
                     LSIDGLEITRPDIRLVVNGEGKTNWEIIKSAPSSEPDAGGLAFELKRYSINRGIISYT
                     DAARGNRVQLDNFTHQGSGNFSAKQFVLDTRTTIDSTDIIFQGNKYLNRADFAADLRL
                     DVDLEKQLFHLRENTVRVNQVVLNFAGWYGMQDGKPQVDVTFNSPETDFKQILSLIPV
                     LYRNKFAELQASGQMAFTGHVHGPIGDGIIPRFEINLDVNNGAFSYAARPARMTNVQL
                     DMAVNNPGTTADDVVVQLRSLHFELNDQPMDLTLQLRTPVSRPWIDTRIKGILDLGQL
                     GQLIVLPSGTILRGRVDADLALQGLVSKQELADLTGSGFLQMDNVYYASPALPQPLQV
                     SRSLIKVASPRLRLESFEAAMGRSDLSANGFLVNPLGFLFANQTLNGTLTVSSRTLDL
                     DPFIEAPSAGLQAFELPDRVHFQMKADLGRVLMQGMEFTKLKGDLLLSGKVLKLNGVK
                     ADFANGSLTADGSYRWLPPAKPALDFNFEADAVRLNGLLQIAIPPSIAPFLNHLSGNF
                     GGTIQLNSALEKSLLPVWESFFNRGNLSITQVSVAAAPLLQKLADAVQINRLRNPTLQ
                     GLNPRYTIQDGRFNVRPFSFTLGDYTFELAGSHGVDMTLDYTLQAAIPAESAEARLNP
                     LLAKYLGGAAGLLKGRRLTMDIAITGTAENPRFNTSIAEALGDTLKQAVQTEAETRID
                     SLKQKTQEKLEEKKEEIADKAKARLKDILDSRKKQ"
     gene            5603..6223
                     /locus_tag="PKM23_07370"
     CDS             5603..6223
                     /locus_tag="PKM23_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNY91332.1"
                     /translation="MKVTVDINLGGDVFHVDEDAYQGLRHYLTSLERAFAEEDGAGEI
                     IADIEGRMGELFKLRLGKYKQVITLGDLEEVMGVLGSPEAISGTAPAGGAARHLLSRR
                     FYRDPDRRILGGVCAGLAAYFSWHPMILRILFIVLALAGSFGIWLYLVLWIVLPEALT
                     TAQKLEMRGDPVTIANIRDSVKAQYEAVREKMHLQSGTNLKTRRET"
     gene            6220..6549
                     /locus_tag="PKM23_07375"
     CDS             6220..6549
                     /locus_tag="PKM23_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNY91333.1"
                     /translation="MNTDNTKAQMRKGILEYCILGILSRSSAYAVDIINELRKAEVIV
                     VEGTLYPLLTRLKNAGLLSYRWEESPQGPPRKYYELTSPGRSYLAELDQAWAELVASV
                     NELRGKK"
     gene            6560..7858
                     /locus_tag="PKM23_07380"
     CDS             6560..7858
                     /locus_tag="PKM23_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91334.1"
                     /translation="MEKTMNINIAGQLFRIDEEAFRSLSRYLETISTRFSNEPGGEET
                     ISDIESRIAEIFGGGQEPPVLVSGEMVREMIEVMGAPEEYADETPGEAPPVERKPLYD
                     PGSLMVRAGQALSACWKACTKIFSVLLRAAALVAGACLSVFGFLLLFAFVFLLFFSNS
                     PMVTGLIEPDIVNLPMLLSIVMNAGFVRPVIILAAMVILIPLAALTYLGIKLIFRLAP
                     ISKPFKVIVFVAWLAALCALCVIIALQVTAYNQDTDIVEKAALDKPPRTLWIAPLKKI
                     AESGYGESAALDGWIFWSKSVDGPLFGTADLDIFCSDTTTGWISVRRGSCGSSESGAW
                     DNARAIDFSWKLSRDTLYLDEHYALPAGASWHGAEVSIDVALPAGTEIRPATGADLAR
                     WRFRRIDPATDRYRIRESRRDVLPEGMVSTRALSDTLRAQ"
     gene            8031..>9550
                     /locus_tag="PKM23_07385"
     CDS             8031..>9550
                     /locus_tag="PKM23_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNY91335.1"
                     /translation="MELAVSDINLQSGIDFVILAGDVTEFGSDAELLLAKKILDQLAK
                     PLYIVPGNHDSKWSESGCNSFVRIFGGESFSFIHHGFFFIGTASGPNMRMAPGLVPRE
                     HLAFLDSALTAAGDLPIIFVNHYPLNEELANYAAVTGRLKKCNIQLSLLGHGHANKLL
                     DFAGIPGVMGRANLRRDDNPAGYTVVTVDSLRIDFAERTPGGETGEPWASVPVMRRQF
                     EPEAVQDGGAAYTVNQRCRNAQVSWRFIDNSDIGSGIVRAGDRAVYANARGEIVCIDP
                     TNGRERWRFATGGKCWATPAISGRNVVCASTDSAVYCLDLSSGREKWRMRTGKSIVAS
                     PLVRAGRIYIGSSEGIFRCIDLAKGCLIWQYDGVDNFVETRPLWYEERICFGSWGNTF
                     YALDAASGRLVWQRQKYTNRMLSPAAVQPVAAHGKIFIVAPDRRMTALDAATGAEIWD
                     SGEYSCRESIGISADGEFVYIKTMKEGDLCAFSSRARAQLLAWRTVTGAGYEIAPTP"
BASE COUNT         1946 a         2923 c         2752 g         1929 t
ORIGIN      
        1 atgccacccc cagaactccg tcgggcatgc ccagggcgat gaaagcggtg agagcgagca
       61 caaccagccc ggccccggga tgttttttgc tgttgttttt ctggatcatg cctctattcg
      121 taccggttga ggaatggtgc tgccgccgct gatcagagtt catcggggaa ctgaataaca
      181 gcccgcctgg gcggcgccga tcagctcctc gggattggtt ccgggtccgg tttcaaagcg
      241 cgaggcgaag gtaaaggccc ctcataagca ccgctgccca ggtgcatggt tcccgccccc
      301 tccttgagcg tgcacttcca ttcagctgaa gatgtgtgta ccgttatacc tctcctcctt
      361 atttaacggt ctgattaaaa gttactcctt ttgaacgcaa atgcaaggag tatttggcgg
      421 ttgcgggcca ggccaatact gatggagaaa taaagcttga tatgttgaat aaaaagagat
      481 agattcgcag ggataggcac cgacggccgc acagatgatc ggccagtgct ctctcgctgc
      541 attcgatctg ccctggctgc tgtgtaaaag gatctgcatg atctcagagg accgctcgct
      601 tgatcggatg tacccggcca aagaagcctt tcattagcct gtcacatata actcggaggt
      661 tgttgcattc cccctggcca gaatgcggaa agtcatcgcc agtacggacg atccacctca
      721 tcttcaggtc cgatcccgcc ggcggaaatt ccctttcccg gccggatcgc tgccgtgctt
      781 tttgacctcg acggcaccct gatggatacc gatgaccaga tgaccgaacg ggtgggccgc
      841 tggctgtcaa ggctgcatct gccccatgcg gagcggctgg cccgcaggat ggtcatgctc
      901 ttcgaatcgc cggtcaacgg cctggtcacc cttctggacc ggatcgggct cgacaagccc
      961 ctgctggact tgttgcagcg gctcagacac ggccaaaaga tgcaccggca tgactttcac
     1021 atcattcccg gtgtagcgga actgctcgcc agcctgcggg gccgctatcg actgggagta
     1081 gtcaccaccc gcagcgagac cgaagccgag gccttcatcg cccagcacca gctgcaggat
     1141 atttttgaat ttgtgatcgg gcgcacctca acccgtcgcc tgaagccgca cccggaaccg
     1201 gtgcgactgg cttcgctgca gctggatatc ccggtcgaac gctgcctgat ggtgggcgac
     1261 accaccatgg atatccgctc cgccgtcgcc gccggggccc ccgccgccgg cgtactctgc
     1321 ggctatggca cccggaagga gctggaacag gcgggcgcaa cggtggtggt ggagcatatc
     1381 accgatctag ccgccctgct caacgctccc gtttttcaga ggggtcaacc ttcctgaagc
     1441 tccttccaac cagcgccgcc cggccgcaaa aagtgcttta ttatgacgcc atcgttttgt
     1501 aattttgctg gacggaagag gcagacatcc aaccactaga ggaaacgata atgaattcgc
     1561 acgcaagagt gcgggctgcg cttgagcaca gaaatcccga caagatcccg atcgatttcg
     1621 gcggccatcg ctcctcgggc atctccgcca tggcctacgc ccggcttaaa agagccctgg
     1681 gcatcaccag cggggatatc tatgtctacg atatgatgca gcagctcgcc atcatcgagg
     1741 agccggtgct gaccgccctc gaggtcgaca ccgtcgagct cggacgcgct ttctggcacg
     1801 aagaacgctt ctggaaggag tggaccctgc cggacggcac cccctgcaag gtacccacct
     1861 cagccaacct cgcgcggcgc ggagaggagt ggtacctgct cgcgccggac tccaccccgg
     1921 tgggagtgca aaagccgggc tgcgatttct ttgagcagat cctctatccc ctcgccgaaa
     1981 gcgattttga acacaccgat ttctcggata tcggcgcggc ctcggcgcac agcatgcgcg
     2041 cggcggcgcc ggtgccgggg tcccatctcg ggatggacga caaggggctg gccgaactca
     2101 gtgaaggcgc caggcggcta cgcgcctcga ccgaccgggc gattcaggcc agcttcggcg
     2161 gcaagatgat tgaagggccc caggcccttt acggcatgga aaattatctc ctctacaccg
     2221 ccctctatcc tgagcaaatc atccgcctct cggaagtgct ctgcgagggc tattgcaaat
     2281 cgctgcgcaa atggcttccg gccgttgccc cctatatcga tctcatcggc ttcggcgatg
     2341 acctgggcac caacaacggg cccatgatca gcccggagaa ataccgactt tactacaagc
     2401 cctatcacgc ccgcatgtgg cggctggtca aggaactggc gccgcacgtc aagatcctgc
     2461 tccactgctg cggcggcatc gaacccctgc tcgaggacct catcgaggcc ggactcgacg
     2521 ccgtcaatcc cgtccagatc agcgcgcggg gcatgcagct ggaaaccctc aagagcaaat
     2581 acgggggacg gatcaccttc tggggcggag gctgcgatac ccagaccatc ctgccccagg
     2641 gaactccgga ccaggtgcgc gaccatgtcc gcggccagat cgagatcatg aagggggagg
     2701 gaggctttgt cttccagcaa gtgcacaaca tcatcgggag ggtgccgatc gaaaatataa
     2761 tggccatgtt cgaaacggtc atccggtttc gctgagcccg gatgctatcg actgcagcac
     2821 aaacctcatt cacccctcat cgctgctctg ccccatgccc ggatagatcg acggctgctc
     2881 accagcatcc gcactaacag cctgctcccc ccatggacag gagaatatcc tgaagttcaa
     2941 tccagcaatt aaataggagc ccttgatcat gaaaaagata tggatcattc tggcggtcat
     3001 tctgctcatc ctcattttat tcgtcgcgct ggcgccggcc tttttcagcg agggcgttgg
     3061 ccgcattgtc gtcaatcagg ctgaaaaaaa gctgaatgct cacatcggat ttaacgggct
     3121 ttcactaagc ctgattaaaa attttccccg gacgacgctc tccattgact ctctccagat
     3181 cataccgctc gagccgtttg ccaaagatac cctgctgcaa tccgagcgga tcacagccac
     3241 tgtggatctg ctcagctatt tcagaaacaa acggctctcg atcgacggcc ttgagatcac
     3301 acggccggat atccgcctgg ttgtcaatgg cgagggcaaa accaactggg agattataaa
     3361 gtccgctccc tccagcgagc cggatgccgg cggtctggcc ttcgaactca agcggtacag
     3421 tatcaaccgg ggcatcatct cctatacgga tgcagcccgg ggcaaccgcg tccagctgga
     3481 caatttcacc catcagggca gcggcaactt ttcggcgaaa cagtttgtcc tcgataccag
     3541 gacgactatt gactcgacgg atatcatttt tcagggcaac aagtatctga atcgggccga
     3601 ttttgccgcc gacctccgcc tcgatgtcga tctggagaag cagcttttcc atttgcgcga
     3661 gaatacggtg cgcgtgaacc aggtggtgct gaatttcgcc ggctggtatg gaatgcagga
     3721 tggaaagccg caggtggatg tgaccttcaa cagcccggag accgacttta agcagatcct
     3781 ctccctgatt ccggtgctgt acaggaacaa atttgccgag ctgcaggcct ccggacagat
     3841 ggccttcacc gggcatgtgc acggcccgat cggcgacggg atcatccccc ggttcgaaat
     3901 caatctcgat gtcaataatg gcgccttcag ctacgccgcc aggccggcca ggatgaccaa
     3961 tgtccagctg gatatggcgg tgaacaatcc gggcacgacg gccgatgatg tggtggtgca
     4021 gctgcgcagc ctccattttg agctgaatga tcagccgatg gacctgaccc tgcagctcag
     4081 gactccggtt tcccgcccct ggatcgatac ccgcatcaag ggcatcctcg acctgggcca
     4141 gctcgggcag ctgattgtcc tcccctccgg caccatcctg cgtggacgcg tcgatgccga
     4201 tctggcgctg cagggcctgg tcagcaagca ggaactcgcc gacttgacgg gctccggctt
     4261 tctccagatg gacaacgtct attacgccag tcccgccctg ccgcagcccc tgcaggtcag
     4321 ccgctcgctg atcaaggtgg cctccccccg cctgcggctg gagagctttg aggcggccat
     4381 gggccggagc gatctctccg cgaacggctt cctggtcaat ccattgggat tcctcttcgc
     4441 caaccagacc ctgaacggca cactcacagt gagcagccgc accctggacc ttgacccctt
     4501 catcgaggcc cccagtgcgg gcttgcaggc ctttgagctg ccagaccggg tccactttca
     4561 gatgaaagcc gatctcggac gcgtgctgat gcagggcatg gagttcacca aactaaaggg
     4621 cgatctgttg ctcagcggca aggtgttgaa attgaacggc gtcaaggccg attttgccaa
     4681 cggcagcctg accgctgacg gcagctatcg ctggctgcca ccagccaaac ccgcgctcga
     4741 ctttaatttc gaggccgatg ctgtgcggtt aaatggcttg cttcagatcg ccataccgcc
     4801 ctcgatcgcc cccttcctga atcacctcag cggcaatttc ggcggcacga tccagctcaa
     4861 ttccgctctg gaaaagagcc tgctgccggt atgggagagc tttttcaatc gcggcaatct
     4921 gtccatcacc caggtttcgg tggccgctgc acccctgctg caaaagctcg ccgatgcggt
     4981 gcagatcaat cgcctgcgca atcccaccct gcaaggcctc aatccgcgct ataccattca
     5041 ggatggacgc tttaatgtgc ggccgttcag tttcactctg ggggattaca ccttcgaact
     5101 ggccggctct cacggcgtgg atatgaccct ggactatacc ctgcaagctg ccattcccgc
     5161 cgaatccgcg gaggccaggc tcaatcccct gctggcgaaa tatctcggcg gagccgccgg
     5221 cctgctcaag ggcagacgcc tgaccatgga tatcgccatc accggaaccg cggagaatcc
     5281 ccggttcaac accagtattg ccgaggcgct cggcgacacc ctcaaacagg cggtccaaac
     5341 cgaggccgag acgcgtatag attccctgaa gcagaagacc caggaaaagc tggaagagaa
     5401 aaaggaagag attgccgaca aggcgaaagc ccggctgaaa gatattctcg acagccggaa
     5461 aaagcagtag aaggagtgcc gacacctctt ttactaaaaa aattgctgta attaaataat
     5521 atgtattgca tagtacctaa aaatatatta tattactgtt gtcagattaa ctggcaatga
     5581 aaggtacttt ggcgaaagaa ctatgaaagt cacagtcgac atcaatctcg gtggagatgt
     5641 ttttcatgtg gatgaggatg cctatcaggg cctccgtcac tacctgacaa gtctggagcg
     5701 ggcctttgct gaagaagacg gcgccgggga gatcatcgcc gatatcgaag gccgcatggg
     5761 cgagctcttc aaactccggc tcggcaaata caaacaggtc atcacgctgg gggacctcga
     5821 ggaggtcatg ggtgttctcg gctccccgga ggcgatcagc ggcaccgcgc cggcgggggg
     5881 agcagcgcgc cacctgctct cccgccgttt ttaccgcgat cccgaccgac ggatcctggg
     5941 cggggtatgc gccggcctgg cggcctactt ctcctggcac ccgatgattc tccgcatcct
     6001 tttcatcgtg ctggccctgg cggggagctt tggcatctgg ctctatctgg tgctctggat
     6061 cgtcctgcct gaggccctga cgacggccca gaagctggaa atgcgcggcg acccggtgac
     6121 catcgccaac atccgggatt ccgtgaaagc ccagtatgag gccgtccgtg aaaaaatgca
     6181 ccttcagtct ggaacaaacc taaagacaag acgggaaaca tgaatacaga caataccaaa
     6241 gcgcagatgc gcaagggtat acttgagtac tgcatactcg gcatactctc gcggagctcc
     6301 gcctatgcgg tggacatcat caacgagctg agaaaggctg aggtgatcgt ggtcgagggg
     6361 acgctctatc ccctgctcac ccggctcaag aatgcgggac tgctcagcta ccgctgggag
     6421 gagtctcccc agggtccccc gcgcaagtat tatgaactga cctctccggg caggagctat
     6481 ctggccgaac tcgaccaggc ctgggcagag ctggtggcat ccgtcaacga gcttcgtgga
     6541 aaaaaatagg cagaccgcca tggaaaaaac gatgaatatc aatatcgcgg ggcagctttt
     6601 ccgcatcgac gaggaggcct ttcgctctct ttcccgctat ctcgagacca tctccacccg
     6661 gttcagcaat gagccggggg gggaagagac catttcggac atcgagagcc gcatcgccga
     6721 gatcttcggc ggagggcagg agccgcccgt gctggtctcc ggcgagatgg tcagggagat
     6781 gatcgaggtc atgggcgctc cggaggagta tgcagacgag acgccggggg aggccccccc
     6841 cgtcgagcgc aagccgctgt acgatcccgg cagcctgatg gtccgcgccg gccaggccct
     6901 ctccgcctgc tggaaggctt gcacaaagat cttttccgtc ctgctcaggg ctgcggcgct
     6961 ggttgctggc gcctgcctct cggtgttcgg ctttcttctg ctcttcgcct ttgtctttct
     7021 gctctttttt agcaatagtc ctatggtcac cggcctgatc gagccggaca tcgtcaatct
     7081 tcccatgctg ctgtcgatag tcatgaatgc tggcttcgtc cgtccggtga tcatcctggc
     7141 ggcgatggtc atcctcatcc ccctggcagc gctcacctat ctcggcatca agctcatctt
     7201 ccggcttgct ccgatttcca aacccttcaa ggtgatcgtc tttgtcgcct ggctcgccgc
     7261 cctttgcgca ctgtgcgtaa tcattgcctt gcaggtcacc gcgtacaatc aggacaccga
     7321 tattgtcgag aaggctgcac tcgacaagcc tccccgcaca ctgtggatcg ccccgctcaa
     7381 aaagatcgcg gagagcgggt atggggagag cgcagccctg gacggatgga ttttctggag
     7441 caagtccgtg gacggaccgc tcttcggcac cgcggatctg gacatctttt gcagtgacac
     7501 cacgaccggg tggatatccg tgcgcagagg gtcctgcggc agctcagagt ccggggcatg
     7561 ggacaatgcg cgggcgatcg atttcagctg gaaactctcc cgcgacaccc tctatctgga
     7621 cgaacactac gccctccctg cgggggcctc atggcatggt gcggaggtca gtatcgatgt
     7681 ggcgctgccc gcaggcacgg agatcaggcc ggccaccggt gcagatctcg cccgctggcg
     7741 attccggcgg attgatcccg ccacggaccg ctaccggatt cgcgaaagca gaagggatgt
     7801 attgccagag ggcatggtct ccacccgggc tttatccgat acgctgcggg ctcaatgaat
     7861 gagggcgagc cggcgatttt cgggataaca ggcgggagga aaatctgaca atcaggaaat
     7921 atgtcgcggc gctcgcttta ctgctgctgg ctgccggctc tccgggacag agctttcagt
     7981 tcgctctcgt caccgacacc cacgtcggtg gcgccacggg tgaggccgac ctggaacttg
     8041 ccgtctcgga tatcaacctt cagtccggga tcgattttgt catccttgcg ggcgacgtca
     8101 ccgaattcgg ttcggatgcg gagctgctgc tggcgaaaaa gatcctcgat cagctcgcca
     8161 aacctcttta tatcgttccg ggcaaccacg actcgaaatg gtcggagagc ggctgtaaca
     8221 gctttgtgcg catcttcggc ggggaatcct tctccttcat tcatcacggt tttttcttca
     8281 tcggcaccgc gtccgggccc aacatgcgca tggcgcccgg actcgtcccg cgcgagcacc
     8341 tcgcttttct cgattcagcc ttgaccgccg ccggcgatct ccccattatt tttgtcaatc
     8401 attatcccct caacgaagag ctggctaatt acgccgccgt gaccggccgg ctcaaaaagt
     8461 gcaacatcca gctttccctg ctcggtcatg gtcacgccaa caaactgctg gatttcgccg
     8521 gcatccccgg cgtgatgggg cgcgccaacc tgcgccggga cgataatccg gccggctata
     8581 cggtcgtcac cgttgattcg ctgcggattg attttgccga gcggacgccc gggggagaga
     8641 ccggggagcc ctgggcgagc gttccggtca tgcgtcgcca gttcgaaccg gaggccgtgc
     8701 aggacggcgg cgccgcttat accgtgaacc agcgctgcag gaatgcgcaa gtgagctggc
     8761 gtttcatcga caacagcgat atcggttcgg gcatcgtccg tgccggcgat cgcgccgttt
     8821 atgcgaacgc ccgcggtgaa atcgtctgta ttgatcccac gaatggcagg gagagatggc
     8881 gttttgccac cggcggtaag tgctgggcca cgccggcgat cagcggccgg aacgtggtct
     8941 gtgcgagcac ggacagcgcc gtttactgcc tcgatctcag ctccggccgc gagaaatggc
     9001 gcatgcgcac gggcaaatcg attgtggcct cgccccttgt ccgggccggc cgcatctata
     9061 tcggttcgtc ggaggggatt tttcgctgca tcgacctggc caaagggtgc ctgatctggc
     9121 agtacgacgg ggtcgacaat ttcgtcgaaa ccaggccgct ctggtatgag gagaggatct
     9181 gtttcggctc ctggggcaac actttttatg ccctcgatgc ggcttccggc aggctggtct
     9241 ggcagcggca gaaatatacc aatcgcatgc tttcaccagc ggccgttcag cctgtggccg
     9301 cgcatggtaa aatctttatt gtcgcaccgg accggcgcat gaccgcgctc gacgcagcga
     9361 ccggcgccga aatctgggat tccggggagt acagctgccg cgaatcgatc ggcatctccg
     9421 ccgacggcga attcgtctat atcaaaacga tgaaagaagg ggatctctgc gccttttcga
     9481 gccgcgcgcg ggcgcagctg ctggcctggc gtacggtcac gggcgccggc tatgagatcg
     9541 cgccgacccc
//
LOCUS       DAOPWU010000112         9515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7240_length_9515_cov_4.669979, whole genome shotgun sequence.
ACCESSION   DAOPWU010000112 DAOPWU010000000
VERSION     DAOPWU010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7240_length_9515_cov_4.669979"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKM23_07390"
     CDS             <1..476
                     /locus_tag="PKM23_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91336.1"
                     /translation="TVGLWSIYTGEHCFDDLLVVSATGDTLLQEGWEKEGRPHKPPSW
                     IDFRHNEQALPLLMAALPDTTFTLLLCHNPETAETAGALGVDLMLSGHTHGGQLRLPL
                     LGSPSLEYKHGRRFIKGFYRIGGLSLYVHSGLGTVYLPLRFLAPPEIALFHISAP"
     gene            605..2146
                     /locus_tag="PKM23_07395"
     CDS             605..2146
                     /locus_tag="PKM23_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNY91337.1"
                     /translation="MKGFRIILLLLLGGILTASAQTKIFIDTEGATQTISRHIYGHFS
                     EHLGRCIYDGLWVGKNSTIPNINGFRQEAVAALLEIGIPNLRWPGGCFADIYHWQDGI
                     GDPKKRPPILNYHWGNVTEDNSFGTHEFLELCELLKCEPYLAANVGTGTPRDLKDWVQ
                     YVNAKGTSPMTQLRARNGRTAPWAVKYWGIGNEVWGCGGNMDAEYYARLYNHYATFCR
                     DYDDTRCVRIACGPYGSDTTWTATLLKHINPGLLQGLSMHYYTVARGDWNKKGSATNF
                     GEEEWFEQLSATLKMEEAIDQNSRVMDRFDPEKKIALIVDEWGDWYDVEPGTEGGFLY
                     QQNSLRDALVAAINFNIFNNRCDRVKMANIAQTVNVLQSVALTRGSELVRTPTFYAFK
                     LFRPHSDATLLPSRIEAELYTFNGKSIPAVTVSASRNKEGVIHISLTNADPKTSQEIV
                     CELKGFSPEYVEGKMITAEAINAFNDFGKAEAVNIQSFDGERIFGNKILLTLPAKSVL
                     MLEAH"
     gene            2202..3125
                     /locus_tag="PKM23_07400"
     CDS             2202..3125
                     /locus_tag="PKM23_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91338.1"
                     /translation="MSTGAGAGQSGAEIESSITYEQIELWRDHTYHRTPRRQVRDIPG
                     ALRFVNETGFCFAFTARHSELPCLWHAACGERHPLYPEHTHSDPFIGLVWQAKDELPA
                     RRALYYGKAIKKRPSLISLEYLPAFYRLIAGDRGEDRYIADYMAGRLSPAAKRIMDAL
                     TERSPQITSELKLSSGCAHPRKRAEFDRGMAELQMGMHVCKIAEFYDPFTFLWERFTS
                     RYQPEVEAARGHSPEQARIRILQQYFRLAGAAAGQDIQRLFAWSPVDIDRTLEALTAE
                     GLIRPVRLEGQNTKKIKYFCFKSFPAAYTEC"
     gene            3172..4698
                     /locus_tag="PKM23_07405"
     CDS             3172..4698
                     /locus_tag="PKM23_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNY91339.1"
                     /translation="MQAVSRRSFLKTSALGLASGALILDGGWLSHANGAAMDYFAAEF
                     GISDGLCEKVLAKALARGGDFADLFFEHTISNYLILEDGKVNRAYGEVALGVGIRTVK
                     GDQVGYGYTQELEEKSMLAAAATAATIADGTTATPPVKFSRISLADRYPLKKLYSAVP
                     LSDKLPLVQSANDKCFALSGEIIKVNATLNDQQKRILIVTSDGVKAEDLQPNSFLAAS
                     VTAERNGRRERAGYNFGGRREFSFYSPALVDELCKEAVGRVLVLFDAVKPPAGEMPVV
                     FGPGVTGILLHEAIGHGMEADFNRKKISTFATMIGKKVAEPFVNIVDDATNERLPGSL
                     NVDDEGTPGQKTLLVENGILTSYLHDKISARHYRVNSTGSGRRQSYEHFVQPRMRNTY
                     MLAGPASPEEVIGSVQKGIYIKDVSNGQVKIGEGDFAFYVSQGQMIEDGKLTAPIKDV
                     NIMGNGPKMLANITMLANDLQMNRGGAGMCGKGGQGVPVGFGQPTCRVQSLTVGGTQA
                     "
     gene            4710..6032
                     /locus_tag="PKM23_07410"
     CDS             4710..6032
                     /locus_tag="PKM23_07410"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNY91340.1"
                     /translation="MNKELFDLASWAIENAKKAGAAASRVAIDRERSVEISYREHKPE
                     NIKEAAKRSLSIEIFADGRFSGQTTSDLRKKALEEFIANAVRTTRLLAEDPYRSLPDP
                     HYYQGRSTADLQLTDPAYSGYTPEERHKTARAMEEACLEAGGDKVISVTAQAQDGENE
                     SVLLASNGFEGGQSSTFYAGVASMTAQDEGERRPNGYHYAVAIRRSEMPGPEEIGRTA
                     ARNTLDLLGAAKIKTETLPVIIRNQDVSRVLSGLLAAMYGANIQQKRSFLADKRGQKI
                     ASEVLTVIDDPLLPGGLGSRTFDNDGMAARRRIMIDKGVLNEFFVDWYYSRKLGWEPT
                     TGGPANLLIPPGSRSVAEIMQELGRGIYITGFIGGNSNSTTGDSSIGILGKLFENGVP
                     VQSVAEMNIAGNHLEFWHKLIEAANDPWPYSSWRTPSLVFKEVVVSGV"
     gene            6267..9035
                     /locus_tag="PKM23_07415"
     CDS             6267..9035
                     /locus_tag="PKM23_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91341.1"
                     /translation="MIKKWFLIISSLVWAVSLQAGVTGKIAGKITDNQTKQPIPGVNV
                     LVEGTSMGAITDISGQYTILNVPPGSYTVRASMIGYTTMRVEGVRVSIDLTTRVNVNL
                     STEVLDITDAITIVAQRPMIQKDEVATKHYISNEEIELQPVDSFQEIAQNQAGVVGSH
                     FRGGRGSEVLVLIDGIPVRDASGEYAGNMGGFTSSVPSYGIEELEVSLGGFGAEYGNV
                     QSGVLNLAMKEGSSRYNGRFRVSTTDFGSASINTPFNRKIYEASLSGPVPMAKKVIPG
                     DLSFSLSGELTDKDHGYYPNQYTYDRTLQGKLTWRISPNYKLTYGTLYNQMDWDQYYF
                     YAAKYGPGAEYQSNQYQYVDNATLYNYLYVIDPSKYRDQQGAINAVSGSLNDKPYTQM
                     QTYYVGGMQDYLWNYEKRSNTNYLVWTHALNPRTYYEVRAQHFFSNYHYATPDVEDRD
                     GDGDTKEDLRWDLTQPGPHPIYRERENNLWWVRGDDPGYLYQGSWTRSVKADLVSQVT
                     QNHLLKGGLEFYNHRTKVENISWSLNLETQRKDIWDEDAYDMGAYIQDKLEFEGIIAL
                     VGLRYDAFNPNGLGDAVYYPADFANPFAEVVGDLPVLLNPQKPSVKHQLSPRIGISHP
                     ITDRDVIHYTYGHYFQRPDLYWLFRNNKYQALTKVGNNIGNPNLNPEKTVAYEIGLEH
                     QFNDDIKGTVTGYYKDISNLVDWMKYVGRSIQNIEMNVFTNADYGNVKGLEFTLSKRL
                     GRFWGGHVNYTYSVAKGRSSDAWGGYGSFTDAKRMNLLDYDQTHTVNANISLRTPGEF
                     GPGLLGVKPLGNWQSNIQFSYGSGLPYSSYGTNKVNDQRLPWTSTTDLKLLRQFKLAG
                     LGLDLFIDVFNLFDRKNVNYINSSQYYEITGDPSVIMLDVDGVNYIRNPQVWDTGRQV
                     RAGVAIQF"
     gene            9048..>9515
                     /locus_tag="PKM23_07420"
     CDS             9048..>9515
                     /locus_tag="PKM23_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91342.1"
                     /translation="MVTVNRYRTTILLLLALLLCITLTAWAVDSPDHKARLAKPGTFE
                     IASPIVPDNWKFHQIGTLWSRVTNFSYMGDDAYEGRTPSCDYPGGSGNSYLYRGTIWL
                     TALVDGTPHSTQGDDHEFSPLDSVHVYSGPGARSEEDTWTRYYDVSAPLASGHF"
BASE COUNT         2072 a         3200 c         2533 g         1710 t
ORIGIN      
        1 gaacggttgg tttatggagc atttataccg gggaacactg cttcgatgat ctgctggtgg
       61 tctccgcaac gggggatacc ctgctgcagg aggggtggga aaaagagggc agaccgcaca
      121 aaccaccctc ctggatcgac tttcgccaca acgagcaggc cctgcccttg ctcatggccg
      181 ccctccccga taccaccttc accctgctct tgtgccataa tccggagaca gcggagacgg
      241 ccggcgccct tggcgtcgac ctcatgctga gcggccacac ccacggcggc cagctgcggc
      301 tgcccctgct gggatccccc agcctcgagt acaagcatgg ccgccgtttc atcaagggat
      361 tctatcgcat cggggggctc tctctttatg tccattcagg gctgggtaca gtctacctcc
      421 ccttgcgctt cctcgcccca ccggagatcg ctctcttcca catcagcgca ccgtaggccg
      481 cagccgctcg accggcacgc tgcagatagt agaacaaaac cggcattaga aaaagaggtt
      541 gcgattccag ccgctgcttt ttacattgaa tcatacctca aggattaccg gaaaggagat
      601 ttacatgaaa ggattcagga tcatcctcct gctgctcctc ggcggcatac tcacggcctc
      661 cgcccagacc aaaatcttca tcgatactga gggcgcaacc cagaccatca gccgccatat
      721 ctacggccac ttttccgagc atctcggccg ctgcatctat gacggcctct gggtcggtaa
      781 aaactccacc atccccaaca tcaacggctt tcgccaggaa gcggtggccg ccctgctcga
      841 gatcggcatc cccaatctgc gctggccggg cggctgcttc gccgacatct atcactggca
      901 ggacggcatc ggcgatccca aaaaacgccc gccgatcctc aactatcact ggggcaatgt
      961 caccgaagac aacagcttcg gcacccacga atttctcgaa ctctgcgagc tgctcaagtg
     1021 cgagccctat ctcgccgcca acgtcggcac gggcaccccg cgcgatctca aggattgggt
     1081 gcagtacgtc aacgccaaag gcaccagccc gatgacccag ctgcgcgccc gcaacggccg
     1141 caccgctccc tgggcggtta aatactgggg tatcggcaac gaggtctggg gctgcggcgg
     1201 caacatggat gccgaatact atgccaggct ctacaaccac tacgccacct tctgccgcga
     1261 ttacgacgac acccggtgcg tgcgcatcgc ctgcggaccc tacggcagcg acaccacctg
     1321 gaccgccacc ctgctgaaac acatcaatcc cggccttctg caggggctct ccatgcatta
     1381 ctacaccgtc gcgcgcggcg actggaacaa gaagggctcc gcaaccaact ttggcgagga
     1441 ggagtggttc gaacagctca gcgccaccct caaaatggag gaggccatcg accaaaacag
     1501 ccgcgtcatg gatcgcttcg atcctgaaaa aaagatcgcc ctgatcgtgg acgagtgggg
     1561 cgactggtac gacgtcgagc cgggaaccga agggggcttc ctctaccagc agaacagcct
     1621 gcgtgacgcc ctggtggcgg caatcaattt caacatcttc aacaaccgct gcgaccgggt
     1681 taaaatggcc aacatcgccc agaccgttaa cgtcctgcag tcggtggccc tcacccgcgg
     1741 cagtgaattg gtgcgcacac cgacctttta tgccttcaag cttttccgcc cccacagcga
     1801 cgcaaccctg ctgcccagcc gcatcgaagc ggaactctac accttcaacg gtaaaagcat
     1861 cccggccgtc actgtttcgg cctcgcggaa caaggagggg gtcatccaca tcagcctgac
     1921 caacgccgac cccaagacct cgcaggagat cgtctgcgaa ttgaagggct tttcccccga
     1981 gtacgtcgag ggcaagatga tcaccgccga ggcgatcaac gcatttaacg atttcggcaa
     2041 agcggaggct gtcaatatcc agtccttcga cggcgagcgc atcttcggca acaagatcct
     2101 gctcaccctg cccgccaaat cagtgctcat gctcgaggcg cactaggcac cccggcctta
     2161 cgggccatcg cgataaacct gtgcttccgg ctcccgcccg tgtgtcaacc ggggcgggag
     2221 ccggacaatc cggagctgaa atagaatcct ccatcacata cgagcagata gagctctggc
     2281 gcgaccacac ctatcaccgt acgccgcggc ggcaggtgcg cgacatcccc ggcgccctgc
     2341 gcttcgtcaa tgaaaccgga ttctgttttg ccttcaccgc gcgccactcc gaactgccct
     2401 gcctctggca tgcggcctgc ggtgagcgcc accccctcta ccccgaacac acccattcgg
     2461 atcccttcat cggcctggtc tggcaggcca aggatgagct gccagcccgg cgcgccctct
     2521 attacggcaa ggcgattaaa aaacggccct cgctgatctc cctggagtat ctgcccgctt
     2581 tttaccggct catcgccggc gatcggggcg aggaccgcta catcgccgac tatatggcgg
     2641 ggagactctc gccggcagcg aagcggatca tggatgccct gaccgaacgc tccccccaga
     2701 tcacctcgga actcaagctc tccagcggct gcgcccaccc gcgcaaacgc gccgaattcg
     2761 accgcggaat ggccgagctg cagatgggga tgcacgtctg caagatcgcc gagttctacg
     2821 atcccttcac cttcctgtgg gaacgcttca ccagccgtta ccagccggag gtcgaggccg
     2881 cccggggcca ctctccggaa caggcccgca tccggatcct gcagcagtat ttcagattgg
     2941 ccggggctgc cgcaggccag gacattcagc gcctcttcgc ctggagtccc gtggatatcg
     3001 accgcacact ggaagcgctc accgccgagg gtctgatccg tcccgtccgg ttggaaggcc
     3061 aaaatacaaa gaaaataaaa tatttctgtt tcaaatcctt ccccgctgcg tatacggagt
     3121 gttagttccc ccctatttcc aatccaacca acccaactaa aggaggatct tatgcaagcg
     3181 gtatcccgaa gatcctttct caaaacctca gccctgggcc tcgcctccgg agcgctcatc
     3241 ctggacggcg gctggctgag tcatgccaac ggagcggcca tggattactt cgcagcggaa
     3301 ttcggcatca gcgacgggtt atgtgaaaag gtgctggcca aagccctggc tcgaggcggc
     3361 gatttcgccg atctcttttt cgagcacacc atttccaact acctcatcct cgaggatggc
     3421 aaggtcaatc gggcctacgg cgaggtcgcc ctcggcgttg gcattcgcac cgtcaagggc
     3481 gaccaggtgg gctatggcta cacccaggaa ctggaggaaa aatcgatgct cgccgcggct
     3541 gccacagccg ccaccatcgc cgacggtacg actgcgactc ccccggttaa attttccaga
     3601 atctccctgg cggaccgata tcccctgaaa aaactctact ccgccgtgcc gctctcggac
     3661 aagctccccc tggtgcagtc cgccaatgac aagtgcttcg ccctctccgg ggaaatcatc
     3721 aaggtcaacg ccaccctcaa cgaccagcaa aagcgcatcc tcatcgtcac cagcgacgga
     3781 gtcaaagcag aagatctcca gcccaacagc ttcctcgccg cctcggtcac cgccgagcgc
     3841 aatggccgcc gggaacgtgc cggctacaat ttcggcggcc gccgggagtt ctcgttctat
     3901 tccccggccc tggtcgatga attgtgcaag gaggcggtcg gccgcgtgct ggtgctcttc
     3961 gatgcggtca aacccccggc cggagagatg cccgtggtct ttggacccgg cgtcaccggc
     4021 attctgcttc acgaggcgat cggccatggc atggaagcgg actttaaccg caaaaagatc
     4081 tcgaccttcg ccaccatgat cggcaaaaag gtggccgagc ccttcgtcaa catcgtcgac
     4141 gacgccacca atgaacggtt gccgggctcc ctcaacgtcg atgatgaagg cacccccggc
     4201 cagaaaaccc tcctggtcga gaatggcatc ctcacctcct acctgcacga caagatctcg
     4261 gcccggcact acagggtgaa ttcgaccggc agcggccgcc gccagagtta cgagcatttc
     4321 gtccagccgc gcatgcgcaa cacctacatg ctcgccggac cggccagccc cgaagaggtt
     4381 atcggcagtg tgcaaaaagg catctacatc aaggatgtca gcaacggcca ggtcaagatc
     4441 ggcgagggcg atttcgcctt ctatgtctcc cagggtcaaa tgatcgagga tggcaagctc
     4501 actgccccca taaaggatgt caacatcatg ggcaacgggc ccaaaatgct cgccaacatc
     4561 accatgctgg ccaacgactt gcagatgaac cgcgggggcg cgggcatgtg cggcaagggc
     4621 ggccagggcg tacccgtcgg cttcgggcaa cccacctgcc gggtccagtc gctgaccgtc
     4681 ggcggcaccc aggcataagg aggaacgcca tgaacaagga acttttcgat ctggcctcct
     4741 gggccatcga gaacgcgaaa aaggccggcg cagccgccag ccgcgtcgcc atcgaccgcg
     4801 aacgctcggt cgagatcagc taccgcgaac acaaaccgga aaacatcaag gaggccgcca
     4861 agcggagcct cagcatcgaa atctttgccg atggccgctt ttccggtcag acgacctcgg
     4921 atctgcgcaa gaaagccctt gaggaattca tcgccaacgc cgtccggacc acccggctgc
     4981 tggcggaaga tccctatcgc tccctccccg atccgcacta ctatcagggc cgctccaccg
     5041 ccgacctgca gctgaccgat ccggcctaca gcggctacac cccggaggaa cggcataaaa
     5101 ccgcccgcgc catggaagag gcctgcctcg aagctggagg cgacaaggtc atctcggtca
     5161 cggcgcaggc ccaggatggc gagaatgaat cggtgctgct cgccagcaat ggtttcgagg
     5221 gcggccaatc ctccactttc tatgccgggg ttgcgagcat gaccgcacag gatgagggcg
     5281 agcgccggcc caatggctat cattacgcag tcgccatccg gcgcagtgaa atgcctggtc
     5341 cggaggagat cggccgcact gccgccagga ataccctgga cctgctcggg gcggcaaaaa
     5401 taaaaaccga aaccctgcca gtcatcattc gcaatcagga tgtatcacga gtcttgagcg
     5461 gcttgctggc ggccatgtac ggcgccaaca tccagcagaa acgctccttc ctcgccgaca
     5521 agaggggcca gaaaatcgcc agcgaggttc tgaccgtgat cgacgatcca ctcctgcccg
     5581 gcgggctggg cagccggact ttcgacaacg acggcatggc ggcgcgcaga cggatcatga
     5641 tcgacaaggg cgtgctgaac gagttcttcg tcgactggta ctacagccgc aagctgggct
     5701 gggagccgac caccggcgga cccgccaacc tcctcatccc gccaggcagc cgttcggtag
     5761 cggagatcat gcaggagttg ggacgcggca tctacatcac cggcttcatc ggcggcaact
     5821 ccaactccac caccggcgac agctcgatcg gcattctcgg caagctcttc gagaacggtg
     5881 tgccggtgca gtcagtcgcc gagatgaaca tcgccggcaa ccatctcgag ttctggcaca
     5941 aactgatcga ggcggccaac gacccctggc cctactcctc gtggcggacg ccgagcctgg
     6001 tcttcaagga ggtggtggtc tccggagtct gaaaccatgg ccgctgctgc cgggcctgcc
     6061 ttcgggtggg tgcgagatta cagcccgggg gatcagcatc tcctccgggc tgtaatattt
     6121 tcatttttgc ttgactttga ttattaaaag cccaatatta gaaatattga acctctgact
     6181 ctttcctcat tcaagacttc atcatgactg cacagatgcc cacagcctac accaaaccaa
     6241 ggtccctaac tcaacggagg cggtgaatga ttaaaaagtg gtttttaatc atcagttctc
     6301 tcgtctgggc cgtttcactg caggccggtg tcaccggcaa aatcgccggc aagatcaccg
     6361 acaaccagac caagcagccc atacccggcg tcaatgtgct tgtggaaggc accagtatgg
     6421 gtgccatcac agacatcagc ggccagtaca ccatcctgaa tgtgccgccg ggaagctaca
     6481 cggtgcgtgc atccatgatc ggctacacca ccatgcgcgt tgagggggtc cgggtctcca
     6541 tcgatctgac cacccgggtc aacgtcaacc tctctaccga agtccttgat atcaccgacg
     6601 ccatcaccat cgtggcgcag cggccgatga tccagaagga cgaggtggcg accaaacact
     6661 acatctccaa cgaggagatc gagctccagc cggtcgactc ttttcaggag atcgcccaga
     6721 accaggccgg cgtggttggc agccacttcc gcggcggccg cggcagcgag gtgctcgtac
     6781 tcatcgacgg catcccggtg cgcgacgcct ccggtgaata cgcgggcaac atgggcggct
     6841 ttaccagttc ggtgcccagc tacggcatcg aagagctgga ggtctcgctc ggcggcttcg
     6901 gagctgaata cggcaacgtc cagtccggcg tcctcaacct cgccatgaaa gagggctcca
     6961 gccgttataa cggccgcttc cgcgtctcca ccaccgattt cggcagcgcc agcatcaaca
     7021 cccccttcaa ccgcaaaatc tacgaagcct ccctctcggg acccgtcccg atggcgaaaa
     7081 aggtgatccc cggcgacctc tctttttcac tctcgggcga actcaccgac aaggaccatg
     7141 gctactatcc caatcagtac acctacgacc gcactttgca gggcaaactg acctggcgca
     7201 tctctcccaa ttacaagctg acctacggca ccctctacaa tcagatggat tgggatcagt
     7261 actatttcta cgccgccaaa tacggccccg gagcggaata ccagagcaat cagtaccagt
     7321 atgtggacaa tgccaccctc tacaactatc tctacgtcat cgatcccagc aagtatcgcg
     7381 accagcaggg tgccatcaat gcggtctcgg gcagcctcaa cgacaagccc tacacccaga
     7441 tgcagaccta ctatgtgggc gggatgcagg actatctctg gaactatgag aagcgcagca
     7501 ataccaatta cctggtctgg acccacgccc tcaatcccag gacctattac gaagtgcgcg
     7561 cccagcactt tttctccaac taccactatg ccacccccga cgtcgaggac cgcgacggcg
     7621 acggcgacac caaagaggat ctgcgctggg acctgaccca gcccggaccc catcccatct
     7681 atcgcgagcg cgagaacaat ctctggtggg tgcgcggcga tgatcccggc tacctctacc
     7741 agggctcctg gacccgctcc gtcaaggcgg acctggtcag ccaggtgacc cagaaccacc
     7801 tcctcaaggg cggcctcgaa ttctacaacc accgcaccaa ggtggagaat atatcctggt
     7861 cgctcaatct cgaaacccag cgcaaggata tctgggatga ggacgcctat gacatgggcg
     7921 cctacatcca ggacaagctg gagttcgaag gcatcatcgc cctcgtcggc ctccgctacg
     7981 acgccttcaa tcccaatgga ctcggagacg ctgtttatta tcctgcggat ttcgccaatc
     8041 ccttcgccga agtggtcggc gacctgcctg tgctgctcaa tccccagaag ccgagcgtca
     8101 aacaccagct cagcccgcgc attggcattt cccatcccat caccgaccgt gacgtgatcc
     8161 actacaccta cggccactat ttccagcggc cggatctcta ctggctcttc cgcaacaaca
     8221 aatatcaggc cctcaccaag gtcggcaaca acatcggcaa tcccaacctc aatccggaaa
     8281 aaaccgtggc ctacgagatc ggtctggagc accagttcaa cgacgacatc aagggtacgg
     8341 tgaccgggta ttacaaggat atctccaacc tggttgactg gatgaaatat gtcggccgct
     8401 cgatccagaa catcgagatg aacgtcttca ccaacgccga ttatggcaac gtcaagggac
     8461 tcgaattcac cctgagcaag cgcctcgggc gcttctgggg cggccatgtc aactatacct
     8521 attcagtcgc caagggtcgc agttccgacg cctggggcgg ctatggcagc ttcaccgacg
     8581 ccaagcgcat gaacctgctc gactacgacc agacccatac ggttaatgcc aatatcagcc
     8641 tgcgcacgcc gggcgagttc ggccccgggc tgctcggggt caaacccctg ggcaactggc
     8701 agagcaacat ccagttcagt tacggcagcg gcctgcccta ctccagctac ggcaccaaca
     8761 aggtcaacga tcagcgcctg ccctggacca gcacgaccga cctcaagctg ctgcgtcagt
     8821 tcaagctcgc cggcctcggc ctcgacctct ttatcgacgt ctttaatctc ttcgaccgca
     8881 aaaatgtcaa ttacatcaac agctcccagt attacgaaat caccggtgat ccctccgtca
     8941 tcatgctcga cgtggatggc gtgaactata tccgcaatcc ccaggtctgg gacaccggcc
     9001 gccaggttcg cgccggcgtg gccattcaat tctaagggag gcccttcatg gttacagtga
     9061 atcgatatcg caccaccatc ctgctcctcc tggcgctgct gctctgcatc acgctgaccg
     9121 cgtgggcggt cgacagccca gaccacaagg cgcgcctggc caaacccggc acttttgaaa
     9181 tcgcctcccc gatcgtgccc gataactgga aattccacca gatcggcact ctctggtcgc
     9241 gggtgaccaa tttcagctac atgggcgatg acgcctacga aggccgcacc ccttcatgcg
     9301 actatccggg cggctcgggc aactcctacc tctaccgcgg caccatctgg ctcaccgccc
     9361 tggtcgacgg caccccgcat tccacccagg gcgacgacca cgagttctcg cccctcgatt
     9421 cggtgcacgt ttactccggt cctggcgccc gctccgagga ggacacctgg acccgctact
     9481 atgatgtctc cgcccccctg gccagcggcc acttt
//
LOCUS       DAOPWU010000113         9428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7317_length_9428_cov_5.036808, whole genome shotgun sequence.
ACCESSION   DAOPWU010000113 DAOPWU010000000
VERSION     DAOPWU010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7317_length_9428_cov_5.036808"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKM23_07425"
     CDS             complement(<1..377)
                     /locus_tag="PKM23_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91343.1"
                     /translation="MFSTWRNSIRTEILFYLIPGLLFILMFFALTIINMSTTQQNEAI
                     IRQTQETTRHYASEVQVQMRAGLEFVETVATVMENYRSRDRAEVNAMLRQFIIRHENF
                     VGLSLGFEPNAFDGRGRRHRCPG"
     gene            complement(410..1861)
                     /locus_tag="PKM23_07430"
     CDS             complement(410..1861)
                     /locus_tag="PKM23_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91344.1"
                     /translation="MPANYDKNPAAQILIIAMLTAGVMILFEFIKTTWWPDLGMWPSH
                     IATIIFITLCAVFASLSVIAKERQLRRQTEMALSWREEAESLVASSDRARRMIFDGVY
                     DAVIIHNAAGIIIDINQSTLDLFQADSQHMIGGWIQDFFVPIAETTEPLTEIWDKVLR
                     GEEQLFHYQARRPSDSSVFDVEVFLRRIQLGEKDLLLANIRDLTELKRMEEFAHLQEQ
                     KLIQADKMASLGVLVSGMAHEINNPNNYVLLNARVLSKAWNDIQPIVEEYYEANGDFA
                     IAGMPYSSARDRLGNLYKGITEGAIRIERIVQHLKDFARQTSDHVFGEVDVNLAVETS
                     VEILRGLIQRSTERFELDCAADLPKLHGNAVHLEQVLVNLITNACQALRSNQEGIRVV
                     TRWESRSESIILSVTDEGEGITGDNLQHIMDPFYTTKREHGGTGLGLSISYNLIKNFG
                     GELKISSTPGCGTSVEVRLPVTTRPAEGVKESA"
     gene            complement(1929..2292)
                     /gene="ssrA"
                     /locus_tag="PKM23_07435"
     tmRNA           complement(1929..2292)
                     /gene="ssrA"
                     /locus_tag="PKM23_07435"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2430..3665)
                     /locus_tag="PKM23_07440"
     CDS             complement(2430..3665)
                     /locus_tag="PKM23_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY91345.1"
                     /translation="MLFREFIEDIKKQKLRAFLTTFAITWGTMVVILLMAFGAGLAFR
                     MREGLLNAADRIVTIWPNQTSVKYQGLPIGRRIRFTEADAELLQQSIPMVAQVCPQQG
                     KHGTRLRNGSRTALTYIEAVYPNFEFMRRMYPAAGGRFLDEMDLQQKRRVLFLGSEIA
                     GELFGDESPIGKSCEVDGLPFTVVGVLPKKLQTSMNNGPDNRRAVIPYSTFHSIYGWR
                     YLNSIILQPANPLEGKRIKEEVFRVLGRKHRFDPADDKALFVWDTAEFVAQQEKVFLG
                     LNIFLGVVGGMTLLIAGVGVANIMYVVVKERTREIGIKRAVGAKRWHIMLQIIFESLL
                     MSSAGGVAGILLAVGIIKMVWMIPAGEGAMEFLGRPLLSNAVMAAAVSLLALIGLFAG
                     IFPARKAARIDPVEALRYE"
     gene            complement(3735..4973)
                     /locus_tag="PKM23_07445"
     CDS             complement(3735..4973)
                     /locus_tag="PKM23_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY91346.1"
                     /translation="MSHLIETIKEIFYYLRQYKARTFMTLFGIVWGTVSIVVLLAFGA
                     GVQRSLSKSMHGMGERIAILWPGRTSMPWMGFNRDRYIHLVEEDAELLRREVREIRSI
                     SPEYGKWNAPLRYGEQVNKPNIAGIYPEYAEMRNIQVQPGGRWINDLDMQERRRVIFL
                     GDGLAKYLFGQGSNPVGQYLYVADSPFLVIGVLAHKLQNSSYSSRDEDRAFIPAATFQ
                     SLFGHRYIDNIVYQIRGPLDSQQAQQRVYEVLGKKKGFDPADKQALGIWDTTEMDKFI
                     YYFSLGFTLFMGLIGVMTLTVGGIGLANIMYVVVQERTAEIGVRRSIGARRSHILSQF
                     LIEAFIIVTVGALVGFILAALIIKGIAALPYEDFVGDPVIDLRVALITVLILGLVGMV
                     AGFFPARKASRLSVIECLRH"
     gene            complement(5026..6129)
                     /locus_tag="PKM23_07450"
     CDS             complement(5026..6129)
                     /locus_tag="PKM23_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNY91347.1"
                     /translation="MTKRNKKKWYWIAAAVAVVALLVVAFGAGSKNGKETGPAKIKVG
                     RMDIIDKALAVGAIEPKNEIAVKSKTSGVVGKLYVEVGDYVQAGAPLLDVRPDPTPLE
                     LAETTRGVEIAEIELSTLSRELERQKELKTKGLISQQEFETLEQHYEQASVRTKIARE
                     RLELLKKGRVQIAGNAIETTVRAPITGYILQKSVNLGDPVVSLTSYQAGTELMKMADM
                     KDLLFRGTLDEIDVGKISEGMPCELAIGALPGKTVKGHVTLISLKARKEENTTLFPVE
                     IAIDDAGGAVLRAGFSANATIIINKKVQVLAIPERVVTFRNDSAFVQISTGAQESKET
                     AIQTGLSDAINIEVISGLQEGQEVLEKKVKPIV"
     gene            complement(6327..7784)
                     /locus_tag="PKM23_07455"
     CDS             complement(6327..7784)
                     /locus_tag="PKM23_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNY91348.1"
                     /translation="MSVVAHLEPKSLWKYFDEIRKIPRGSGNEKAVGDYVLKVAKDLG
                     LKAVRDKEGNVIIYKKAAPGFEKSPAVVLQGHLDMVNEKHSTSKHDFDKDPINLILEG
                     NWLLADGTTLGADNGIGVAAALAVLEDDKLKHGPLEVVFTVDEERGLNGARALTPDLL
                     RGRLLLNLDSEELGNFSIGCAGGADTHLTLPLKRTEAKGDQVLYLHIHGLRGGHSGID
                     IHEGRGNAVKILNRLIWQLSREFKIEMVSFNGGDKHNAIPREAFSEIVVRKRDGAKVT
                     AWLEKAMEEIHTEFKPVEAKIELTIEVLKGKAPKVLEAASMEKLLGLIFALPHGPLAM
                     SRSMQGLVETSNNVAAIKSSAARAIILCSSRSSNSAALRATRDKLAAIARMTGAKVEQ
                     PEGYPGWMPNLDSPLLKVTLDTYMEVTGKKAVYSAIHAGLECGIIGEKFPGMDMISFG
                     PDLKHPHSPEEKVNVKTVAKFYEHLLRIIETLAKS"
     gene            complement(7803..8345)
                     /locus_tag="PKM23_07460"
     CDS             complement(7803..8345)
                     /locus_tag="PKM23_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNY91349.1"
                     /translation="MPPSKQKVFVITTGGTIEKIYDEESGTLTNRGSLLQQMLGRLRL
                     PYTTIQSYDLLCKDSLEMTDKDRQMILFAVENLLEHSAPILILHGTDTMELTADYLHK
                     NLPELRVPIILTGAMKPFGFENSDALQNFTEALLACSLISPGLYISMHGCVHPLPGVF
                     KNREKGTFEKREQNPSSFSV"
     gene            8598..9068
                     /gene="greA"
                     /locus_tag="PKM23_07465"
     CDS             8598..9068
                     /gene="greA"
                     /locus_tag="PKM23_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNY91350.1"
                     /translation="MKPLYLTKEQLEKLQTELHHLKRNERTKVIKEIAEAREKGDLSE
                     NAEYDAAKEKQLLIERKIARLEETLSRARILSAEMMNNDQVHVGSRVVLVDEGTQEET
                     VYELVPTAEFHSYDLDAISIDSPVGKALVGKAVDDIVSIRVPAGEITYRVKKIS"
BASE COUNT         1839 a         2586 c         3009 g         1994 t
ORIGIN      
        1 caccccgggc agcgatgccg acggccgcgg ccgtcgaagg cgttgggttc gaagcccagg
       61 ctcaggccga caaagttctc atgacggatg atgaactggc gcagcatggc gttcacctcc
      121 gcccggtccc gggaacgata gttttccatc acggtcgcca cggtttcgac aaattcgagg
      181 ccggcgcgca tctgcacctg cacctcggag gcgtagtggc gtgtggtctc ctgggtttgc
      241 ctgatgatcg cttcattctg ctgggtggtg ctcatgttga tgatcgtcag ggcgaagaac
      301 atcaggatga agagcagtcc cggaatcaga tagaagagga tctctgtgcg gatggagttg
      361 cgccatgttg aaaacatctc aacctctttg gtagatttga tcccgacccc tatgcactct
      421 cttttactcc ttctgcgggc cgggtggtga caggcaggcg gacctcgaca ctggtgccgc
      481 agccgggagt ggatgatatc ttgagttcgc cgccgaaatt cttgatcagg ttgtaggaga
      541 tggatagccc cagtccggtg ccgccgtgtt cgcgcttggt cgtgtaaaag ggatccatga
      601 tatgctggag gttgtccccg gtgatgccct cgccctcgtc ggtgaccgag agaataatgc
      661 tctcgctgcg gctctcccag cgggtaacga cccggatccc ctcctgattg ctgcgcaggg
      721 cctggcaggc gttggtgatc aggttgacga ggacctgttc gagatggacg gcattgccgt
      781 gaagtttggg cagatccgcg gcgcagtcga gttcgaagcg ctcggtgctg cgctggatga
      841 gtccgcgcag gatctccacc gaggtctcca ccgccaggtt gacatccacc tcaccgaaga
      901 catgatcgct ggtctggcgg gcaaaatcct tgagatgctg gacgatgcgt tcgatgcgga
      961 tggctccttc ggtgatcccc ttgtagagat tgccgaggcg gtcgcgggcg gaggagtagg
     1021 gcatgccggc gatggcaaag tcgccgttgg cctcgtaata ctcctccacg atcggctgga
     1081 tgtcattcca agccttgctg aggacgcggg cgttgaggag gacgtagttg ttgggattgt
     1141 tgatctcgtg ggccatgccc gagaccagca cgccgagcga agccatcttg tccgcctgga
     1201 tgagcttctg ctcctgcaga tgggcgaatt cctccatgcg tttcagctcg gtgagatcgc
     1261 ggatgttggc gagcagcaga tccttttcgc cgagctggat gcggcggaga aagacctcga
     1321 catcaaaaac ggagctgtcc gaggggcgcc tcgcctggta gtggaagagc tgctcttcac
     1381 cgcgcagcac cttgtcccag atttcggtca gaggctccgt ggtctcggca atagggacga
     1441 agaaatcctg aatccacccc ccgatcatgt gctggctgtc ggcctgaaag aggtcgaggg
     1501 tgctttgatt gatgtcgatg atgatgccgg cggcattatg aatgatcacc gcatcataga
     1561 cgccgtcgaa gatcatgcgc cgggcgcgat ccgaggaggc gaccaggctc tcggcctcct
     1621 cccgccagct cagagccatc tcggtctggc ggcgcagctg gcgctccttg gcgatgaccg
     1681 agaggctggc gaacacagcg cagagggtga tgaaaatgat ggtggcgata tgggacggcc
     1741 acatgcccag atccggccac cacgtggtct tgatgaattc gaagagaatc atgacccctg
     1801 cggtgagcat ggcgatgatc aggatctggg cggccggatt tttatcataa ttggcgggca
     1861 tggtctgagt atactaaaaa ctgtccaaca aagcaataaa aaaagccctg tcaaagcgac
     1921 agggcttgtg gtggagacgg tgggagtcga acccacgtcc gaaaacccgg ccatagcagc
     1981 cactacatgc ttgttccgct gctgaatctc gatcgcttca ggcgcaacgg accaggccga
     2041 agcgatccag ccctgtaagg tttcgcctcc tggcccagag catgccggaa ggctagcccg
     2101 cagagatcga cgttcatccg cagcccctgc aggcacaaac cgcggtgaac gaggccgcct
     2161 ttaattaggc agccagtgca tacgaataat cgtctgcatt tacttgtttt cccggatgat
     2221 taacgaggaa accggggtcc tcggcatgcg tccactacca ccatgattcc cgtcgaagcc
     2281 agattcgtcc ccatgtaaag ctgaaacgat tccctggcgg gatttgttcc ccctaatgta
     2341 gcaaatttcc ggatgccggg caagagcttt gttgacgcca ggatcgggcc ggccggctgc
     2401 cgctgccggc atccgcagcc ggccggtgct cactcgtagc gcagggcctc gaccgggtcg
     2461 atgcgcgccg ccttgcgcgc cggaaagatc ccggcgaaca ggccgatgag ggcgagcaga
     2521 gagaccgcag ccgccatcac cgcattcgaa agcagcgggc ggccgaggaa ctccatggcc
     2581 ccctcaccgg ccgggatcat ccagaccatc ttgatgatcc cgacagcgag caggatgccg
     2641 gcgacccctc cggcgctgga catcagcagg gactcaaaga tgatctgaag catgatgtgc
     2701 caccgcttgg cgccgactgc ccgcttgatg ccgatctcgc gggtgcgctc tttcaccacc
     2761 acgtacatga tgttggccac accaaccccg gcgatgagca gggtcatgcc gccgactaca
     2821 cccagaaaga tgttgaggcc gagaaagacc ttctcctgct gggcgacgaa ctcggccgtg
     2881 tcccagacaa agagggcctt gtcatccgcg ggatcgaagc ggtgtttgcg gcccagcacc
     2941 ctgaagacct cctccttgat ccgcttgccc tcgaggggat tggccggctg gagaatgatg
     3001 ctgttgagat agcgccagcc gtaaatgctg tgaaaggtgg agtaggggat gaccgcgcgg
     3061 cggttgtccg gcccgttgtt catcgaggtc tgcagctttt tcggcaggac gccgacgacg
     3121 gtgaagggca ggccgtccac ctcgcagctc ttgccaatcg ggctctcatc cccgaaaagc
     3181 tctccagcga tctcgctgcc gagaaagagg acgcgccgtt tctgctgcag gtccatctcg
     3241 tcgagaaagc ggccgcctgc ggccgggtac atccggcgca tgaactcgaa attgggatag
     3301 accgcctcta tataggtcag ggcggtgcgg ctgccgttgc gcaggcgggt gccatgtttg
     3361 ccctgctggg ggcaaacctg cgcgaccatg gggatgctct gctggagcag ctccgcgtcg
     3421 gcctcggtaa agcggatgcg acgaccgatc ggcagcccct ggtatttgac gctggtctgg
     3481 ttgggccaga tggtgacaat gcggtcagcc gcattgagca gcccctcacg catgcgaaag
     3541 gcgaggccgg cgccgaaggc catcagcagg atcaccacca tggtgcccca ggtgatggcg
     3601 aaggtggtga ggaaagcgcg cagcttttgc tttttgatgt cctcgatgaa ttctctgaac
     3661 agcatccaga tatgcatagc gacgaccttg tgctgggtta aggagctctc caatgccggg
     3721 ttggtgcagg cccgtcagtg gcgcaagcat tcgatgacgc tgagccggga ggccttgcgc
     3781 gccgggaaaa agccggccac catgccgacc aggccgagga tgaggacggt gatcagggcg
     3841 acccggagat cgatgaccgg atcgccgacg aaatcctcat agggcagggc ggcgatgccc
     3901 ttgatgatca aggcggcgag gatgaagccg acgagcgccc ccaccgtgac gatgatgaag
     3961 gcttcgatga ggaactggct gaggatatgg ctgcgccggg cgccgatgga acggcgcaca
     4021 ccgatctccg ccgtccgctc ctggacgacg acgtacatga tattggccag accgatcccc
     4081 cccacggtca gggtcatgac gccgatcagc cccatgaaga gggtgaagcc gagggagaag
     4141 taatagataa atttatccat ttcggtggta tcccatatgc ccagggcctg cttgtcggcg
     4201 ggatcgaaac ccttcttctt accgagcact tcatatacac gctgctgcgc ctgctgcgaa
     4261 tccagggggc ccctgatctg gtagacgatg ttgtcgatat agcggtggcc gaagagtgac
     4321 tgaaaggtcg cggccgggat gaaggcgcgg tcctcatcgc gcgagctgta ggaggagttt
     4381 tgcagcttgt gggcgagcac gccgatgacc agaaagggcg aatcggcgac atagagatac
     4441 tggccaacag gattgctccc ctggccgaag aggtatttgg ccaggccgtc gccgagaaag
     4501 atcacccgcc tgcgttcctg catatccagg tcgttgatcc agcgtccgcc cggctgcacc
     4561 tggatattgc gcatctccgc gtattcgggg tagatgccgg cgatgttggg cttgttgacc
     4621 tgctcgccgt agcgcagagg agcgttccat ttgccatact ccgggctgat gctgcggatc
     4681 tcccgcacct cccggcgcag gagttcggca tcctcctcga cgaggtggat gtagcggtcg
     4741 cggttaaagc ccatccaggg catgctggtg cggccgggcc agaggatggc aatgcgttca
     4801 cccatgccgt gcatgctctt gctcagggat ctctgcaccc cggcgccgaa ggcgaggagc
     4861 acgacgatgc tgaccgtccc ccagacgatg ccgaagaggg tcatgaaggt gcgggccttg
     4921 tactggcgca ggtagtaaaa gatctctttg atggtttcga tcagatggct cataatcgct
     4981 gccttgcaat ggtttacgcc ttttacgggc gggtgcggcc cgcggctaga cgatcggctt
     5041 gaccttcttc tcgagcacct cctgcccctc ctgcaggccg gagatcacct cgatgttgat
     5101 ggcatcgctg aggccggtct ggatggccgt ctccttgctc tcttgggctc ccgtgctgat
     5161 ctggacgaag gccgagtcgt tgcggaaggt gacgacccgt tccgggatgg ccagcacctg
     5221 cacctttttg ttgatgatga tagtggcgtt ggcggaaaac ccggcgcgga gcacggcgcc
     5281 gcccgcgtca tcgatggcga tctcgaccgg gaagagggtg gtgttctcct ccttgcgcgc
     5341 tttgagcgag atgagggtga catggccctt gacggtcttg cccggcaggg cgccgatcgc
     5401 cagctcgcag ggcatgccct cactgatctt gccgacgtcg atttcgtcga gggtgccgcg
     5461 gaagagcaga tccttcatat cggccatttt catcagttcg gtgccggcct gataggaggt
     5521 gagggagacg accgggtcgc cgaggttgac cgacttttgc aggatatagc cggtgatcgg
     5581 cgccctgacc gtggtctcga tggcgttgcc ggcgatctgg acgcgtccct tcttgagcag
     5641 ctcgaggcgt tcccgggcga ttttggtgcg caccgaggcc tgttcgtaat gctgctcgag
     5701 ggtctcaaac tcctgctgcg agatcagccc cttggtcttg agctctttct ggcgctccag
     5761 ctcccggctg agggttgaga gctcgatctc ggcgatctcc acgccgcggg tggtctcggc
     5821 cagctccagc ggggtgggat cgggacggac atcaagcagg ggagcgccgg cctggacata
     5881 atcgccgacc tcgacgtaga gcttgcccac cactcccgag gtcttggatt ttacagcgat
     5941 ctcattttta ggctcgatcg cccccacggc cagggccttg tcgatgatat ccatgcgtcc
     6001 gaccttgatt ttcgccggac ccgtttcctt gccgttcttc gaaccggctc cgaaggcgac
     6061 cacaagcagg gccaccacgg ccacagctgc ggcgatccag taccattttt tcttgtttcg
     6121 ctttgtcata ggggaagact cccaaggata agaccatatt gccgggggtt gtttcatcga
     6181 tatatacggc ggggtatctc aaattgttac acggcatggc cgatccggga ggcgatataa
     6241 aaaaagcccc gcagccgaag ctgcggggca ggatgaaaag gagatgcgca gagccccggg
     6301 gtgagccggt tgactccgcg ggggggtcag gatttggcga gggtttcgat gatccgcagc
     6361 aggtgctcat agaatttggc cacggttttg acgttcacct tctcctcggg ggagtggggg
     6421 tgcttcaggt ccgggccgaa ggagatcatg tccatgccgg ggaacttttc accgatgatg
     6481 ccgcattcca ggccggcgtg gatggccgag tagaccgcct ttttgccggt aacttccatg
     6541 taggtgtcga gagtgacctt gagcaggggc gagtcgaggt tgggcatcca gccgggatag
     6601 ccttcgggct gttccacctt ggcgccggtc atgcgggcaa tggcggccag cttgtcgcgg
     6661 gtggcgcgca gggcggcgct gttggagctg cggctgctgc agaggatgat cgccctggcg
     6721 gcgctcgact tgatggcggc gacattgttg gaggtctcca ccagcccctg catgctgcga
     6781 ctcatggcca gagggccgtg gggcagggca aagatcaggc cgaggagctt ctccatcgat
     6841 gctgcctcga gcaccttggg cgccttgccc ttgagcactt cgatggtcag ctcgattttg
     6901 gcttcgaccg gcttgaactc ggtgtggatc tcctccatcg ccttctccag ccaggccgtg
     6961 accttggcgc catcccgctt gcgcacgacg atttcggaaa aggcctcgcg cgggatagcg
     7021 ttatgcttgt cgcccccgtt aaagctcacc atctcgattt tgaactcgcg tgagagctgc
     7081 cagatcaggc gattgagaat cttgaccgca ttgccgcgcc cttcatgaat gtcgatgccg
     7141 gagtggccgc cgcgcaggcc gtgaatgtgc aggtagagca cctggtcgcc cttggcctcg
     7201 gtgcgtttga ggggcagggt gagatgggta tcggcgccgc cggcgcagcc gatcgaaaag
     7261 ttgcccagtt cctcgctgtc gagattgagc agcaggcgtc cgcggagcag atcgggggtc
     7321 agggcgcggg cgccgttgag gccccgttcc tcatcgacgg tgaagaccac ttcgaggggg
     7381 ccgtgtttca gcttgtcatc ctcgagaacg gccagggcgg cggcgacacc gatgccgttg
     7441 tccgcgccca gggtggtgcc gtcggcaagg agccaattgc cctcgaggat caggttgatg
     7501 ggatccttgt caaaatcgtg cttcgaggtg gagtgtttct cgttgaccat gtcgagatgg
     7561 ccctggagga cgactgcggg ggatttttca aagccggggg cggccttttt gtagatgatc
     7621 acgttgccct ctttgtcgcg gacggctttc aaaccgaggt ccttggccac cttgaggaca
     7681 tagtcgccga cggctttttc gttgccggag ccgcgcggga tcttgcggat ttcatcgaaa
     7741 tatttccaaa gggatttggg ttcaagatgc gctacgacag acatgggacc tcctgattag
     7801 ggttagacag aaaaactgga cggattctgc tctctttttt caaaggttcc cttttcacgg
     7861 ttcttgaata cgccgggcag gggatgcacg cagccgtgca tgctgatata gaggccggga
     7921 gagatgaggg agcaggcgag aagggcctcg gtgaaattct gcaaggcgtc ggagttttca
     7981 aaaccgaagg gtttcatggc gccggtcagg atgattggga ccctgagttc gggcagattt
     8041 ttatggagat agtcggccgt cagctccatg gtgtcggtgc cgtgcagaat gaggatgggg
     8101 gcgctgtgct ccagcaaatt ttcgaccgcg aagaggatca tctggcggtc cttgtcggtc
     8161 atttcgaggg aatccttgca gagcaggtca tagctttgaa tggtggtata gggcaggcgc
     8221 agccggccga gcatctgttg caggagggag ccgcggttgg tcagggtgcc gctctcttcg
     8281 tcatagatct tttcgatggt gccgccggtg gtgatgacga acaccttttg tttgctgggg
     8341 ggcataattc ccttctctca agtgaagtcc ctccaattta acataagctg gaaaaaaagc
     8401 aaggtatttt gtttggaggc gggttcgcgc tggatcaaat cttcagattt tacttgacaa
     8461 atttcgtatt ttttattagt ttaagagcac gcaaaccgaa tcggtgcttt cacgccggtt
     8521 cggtttgcgt ttttattccg ctcggaatga gcgtggagaa cagggtgtta accaaatgag
     8581 agtgaagagg cttctgcatg aaacctttgt acctgaccaa agagcagctg gagaaactgc
     8641 agaccgaact gcatcattta aagcgcaacg agcgcaccaa ggtgatcaag gagatcgcag
     8701 aagcgcgtga gaagggtgat ctgagcgaga atgcggaata tgatgccgcc aaggagaagc
     8761 agcttttaat cgaacgcaag attgcccgtc ttgaggaaac tctctcccgg gcgcgtattc
     8821 tcagcgctga aatgatgaac aacgaccagg tccatgtcgg cagccgtgtg gtgctggtcg
     8881 atgagggtac ccaggaggag acggtttacg aactggtgcc cacagctgaa ttccattcct
     8941 atgatctcga tgccatttcg atcgattcgc cggtgggcaa ggccctggtg ggcaaggcgg
     9001 tcgatgatat cgtttcgatc cgggtaccag ccggcgagat cacctatcga gtgaagaaga
     9061 tttcctgagc gaggtgcgga gggcgctgag tggccctcct tcatatactg attaagcagc
     9121 cgggggccgc ggagagcggc agactccgat ggatatggca gcgaaccttc cacctatcat
     9181 tcagctggca atatttgtgc gcactctccc ggagagccgg gcggcccgag tttcttttga
     9241 aaccgggctt ttttgtggcc agattttcca tggagaggca ggatttggct gattgtggac
     9301 tggattttgc ctattgtaaa ccggtaactg cgagcaaaaa atgcatctat ttgatattaa
     9361 acaaattaaa taatagtcgg gtgatatccc gaatccgccc aatccaggga gaatgtcata
     9421 ctatgaaa
//
LOCUS       DAOPWU010000114         9409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7330_length_9409_cov_4.412444, whole genome shotgun sequence.
ACCESSION   DAOPWU010000114 DAOPWU010000000
VERSION     DAOPWU010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7330_length_9409_cov_4.412444"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..1412)
                     /locus_tag="PKM23_07470"
     CDS             complement(318..1412)
                     /locus_tag="PKM23_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNY91351.1"
                     /translation="MAAKFLKRSHKYKATTLPYRLFVPDKYDPSRTYPLILTLHGAGE
                     RGLDNETPLKAHRLGMVWADSLLQSQNPCFIVVPQCPPERQWVNTDWSKGSYSLTNVG
                     ISFELSTVMNLLDSLSSEYAINTDRLYVTGLSMGGYGTWDLITRFPNRFAAAVPMSGG
                     ADTCMAASLRNMPLWVFHGKEDQSVPVSASREMIEALLHSDRACVFTEYDYRSGTSVR
                     MADSVLTEKVAYNAPLLYTEYVGKGHAIWQESYAIPQLARWLFAQKRTAEAAQAKVPQ
                     IDIMEIAFNYPNPFNPSTTIEYSLKKSTLVAIDILNLAGQKIVQLASGRQEAGRHFVR
                     FDAKDLPSGNYFFRIITPDWVQHGNMILLK"
     gene            complement(1612..1685)
                     /locus_tag="PKM23_07475"
     tRNA            complement(1612..1685)
                     /locus_tag="PKM23_07475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1649..1651),aa:Arg,seq:tct)
     gene            complement(1861..2223)
                     /locus_tag="PKM23_07480"
     CDS             complement(1861..2223)
                     /locus_tag="PKM23_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91352.1"
                     /translation="MTREKSLEAMLALVFGCLLLSLLLDIKLLLYIGLLLGGIGLLMA
                     KTSRALARLWYKLSQALGFVISKVLLSLVFFIFLLPMALLSRVFRPDLLQRRRKNTGS
                     YFVVRNYSYQSKDLKNPW"
     gene            complement(2236..2385)
                     /locus_tag="PKM23_07485"
     CDS             complement(2236..2385)
                     /locus_tag="PKM23_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNY91353.1"
                     /translation="MDFLMDLWNFLRERKKWWLLPVIIVLLLLGILIIIGGSSAVAPF
                     IYTIF"
     gene            complement(2500..4362)
                     /locus_tag="PKM23_07490"
     CDS             complement(2500..4362)
                     /locus_tag="PKM23_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HNY91354.1"
                     /translation="MKILGLSAFYHDSAAAILQDGQILAAAQEERFTRKKHTAEFPSQ
                     AARYCLDFAGFSIDELDAVVFYDKPLLKFERLLETYYGFAPRGVVSFMASIPVWLREK
                     MFLKRVIHEELGKIELYDRHRLHMLFPEHHLSHAASAFFPSRFKEAAILTIDGVGEWA
                     TSTIGFGRDHTIEIHKELHFPHSVGLLYSAFTYYLGFVVNSGEYKLMGLAPYGNPEGA
                     ATRKYIALIREHIVDIKEDGSIWLNQDYFNYATGLRMTREEKWHQLFGFPVRKPESPL
                     EQHHCDLGFAIQAVTEEIVLKMAEEAQRLTGARNLCMAGGVALNCVANGKIIKKRLFD
                     DIFIQPAAGDAGGALGAALAAHHIYFGQERNPAPTMDAMQGAFLGPEYSAIDIELMAR
                     KNKAVYHKYTSFTQLVDEVADLLASGQVVGWMQGRMEFGPRALGNRSILGDARNPEMQ
                     KKLNLKIKYRESFRPFAPSVLDEDNTSFFELDMPSPYMLLIADVVSSRRRPLPDHYHD
                     LPLWERLYHQRSDVPAITHLDFSARIQTVHRETNEPYWTLLRRFKEKTGYGLLVNTSF
                     NVRGEPIICTPVDAYKCFMRTEMDYLVIGDYLFVKTEQPNYQDTENWKETFKLD"
     gene            complement(4605..6275)
                     /locus_tag="PKM23_07495"
     CDS             complement(4605..6275)
                     /locus_tag="PKM23_07495"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNY91355.1"
                     /translation="MLQTMNNCIVPGGVPSALQRCTRRAALVLLLLMGGLSALPAQTT
                     GVALRDSNLPIILIDTGGKEIPDLVRIRATMKIIYNGAGRRNAITDPPNNYDGLIDIE
                     LRGSSSMAYPKKGYRLETVTTAGDNRNVSLLGMPKENDWILYASYDDQSLMRNVLAYR
                     ISSEIGRYAPRVKYCELVLNNNYRGVYVFMEKIKQDKNRVNVTELESADISGDAVTGG
                     YVWKFDKEEGEQTAGWTSMQGLYYQYHDPKAEDLVSAQKSYLRGVMNVFESVMLMPTR
                     GDTLFGYPRYIDVDSFVDHFILNEFCKNIDAYRISFFMHKDRDSKGGKIIAGPIWDFN
                     LTFGKTWYAEDAGRVDEWEIDHDIYHPGDWPSVPFWWELLGHDPYFAGKVRSRWAELS
                     QTILLPANLDLRIDQISDTLAEARARDVRRWPEPSAAHSYEVEIATLKSWIRGRTLWI
                     NNHLWRLAPVNGGEEEASPGELLLQQNYPNPFNQSTTIRFTLPRPARVSLRLFSVSGR
                     EVATLAAGNWPAGAHSLTWDGGGQASGLYLCRLEAAGGIQTRTLILLR"
     gene            complement(6378..7403)
                     /locus_tag="PKM23_07500"
     CDS             complement(6378..7403)
                     /locus_tag="PKM23_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNY91356.1"
                     /translation="MKRITIEDVASKAGVSKGTVSAVLNGKNSVKPHTRERILAVMKE
                     LNFRPRGMARNLKNGSEDRSIGIIIKDMNYPFYTSIASGVRAYAVAHGYQVIITSSEN
                     DHECEKKFTHLFSTKDIKGAIIAPIVEGQAEIEHLFKLKMINYPFVLLEEVKGIQANA
                     VVIDNLKAIRQAVKYLIDGGHRRIVHFAGPPQSSHTQERIEGFRDAFSESTLAFTGEM
                     IISIGSRHEESYHNALAWFRSLERTEYPTAILCFNDQQALAVMSALRELNIRVPEEIS
                     IIGNDDIDYAKLYPVPLTTIRAPQMEIGRRAAEILIRNIESPLLLPPERILLETELVV
                     RNSSRTL"
     gene            7745..8989
                     /locus_tag="PKM23_07505"
     CDS             7745..8989
                     /locus_tag="PKM23_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HNY91357.1"
                     /translation="MKNTRRYLVIASLICLALALLLSCGKPLLRHGAEATSHYPNLFT
                     DLLGVGESLVTERLESAFQQLFYGDDRTQRIYYPAGPDMAYIEDILHNDVRTEGISYG
                     MMIAVQLDKKAEFDRLWKWAKTYMQHQEGPSKGFFAWHCTPAGEKLSVSAAPDGEEWI
                     VTALFFASHRWGDGPGIYNYRAEAQAILDAMLSKEPASDLQAVITNMFNREHRQVVFV
                     PNGEADGFTDPSYHLPHYYELWARWADKEKAFWQDAAAVSREFLKKAAHPVTGLTPDY
                     ANFDGTATDAPWGGGHDAFRFDAWRTAMNVAVDWVWFARDEWAIQQSNRLLEFFNDEG
                     LARYVNQYALDGQPLSEERSPGLVAMNGTAALAATTPLRTGFVRAVWEQPVPDGPGRY
                     YDGLLHMLALLQLGGQFRIYAP"
     gene            9146..>9409
                     /locus_tag="PKM23_07510"
     CDS             9146..>9409
                     /locus_tag="PKM23_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91358.1"
                     /translation="MFGVLLPLLPAAAALPYITETPQPGSFTLAEPGRSAYLVASSRE
                     YPGVLRVAGHLQNDLARVTGSAPALLLDTPPAAGDVILFGTLGV"
BASE COUNT         1945 a         2650 c         2756 g         2058 t
ORIGIN      
        1 aatcgcctat tttgggcagg ttacccccga aatccggatc ccatcttgac tcctgacaca
       61 gcgtacccta tccctctgtg cgatttccaa catgctctcg gcctgtgccc tcgtcatgag
      121 gccatttatt ttacacggta tatctggcag gtaaaatata aattttattt aattattttt
      181 aggtgggaac gggattgtgg ggagtatcaa ggaaaatcgc ctattctgag cggtcaggag
      241 gaggaaaaaa acagcccctc tccggcggcc gggaaggggc ttgaaaatca caggatgcag
      301 gacggagcgc ctccacccta tttgagcaga atcatgttcc cgtgctgcac ccagtccggg
      361 gtgatgatac ggaaaaagta attgcccgaa ggcagatcct tggcatcaaa gcggacgaaa
      421 tggcgcccgg cctcctgccg gcccgaggcg agctgcacaa tcttctgacc cgccagattg
      481 aggatgtcaa tcgcgaccag ggtcgatttt ttcagggaat actcgatcgt cgtcgaggga
      541 ttgaagggat tgggatagtt gaaggcgatc tccatgatgt cgatctgggg taccttggcc
      601 tgcgccgcct ccgccgtccg cttctgggcg aaaagccatc ttgccaactg cggaatggca
      661 taggactcct gccagatcgc gtgccccttg ccgacatact cggtgtagag cagcggtgcg
      721 ttataggcga ccttttccgt gagcacactg tctgccatcc ggacggaggt gccggaacgg
      781 taatcgtatt ccgtaaagac acaggcgcgg tcggaatgga gcagggcctc gatcatctca
      841 cgtgaggcgc tgaccgggac actctggtcc tccttgccgt ggaagaccca gaggggcatg
      901 ttgcgcaggg aggcggccat gcaggtatcc gcaccgccgc tcattggcac cgccgcggcg
      961 aagcggttcg gaaagcgggt gatgagatcc caggtgccgt acccgcccat ggagaggccg
     1021 gtgacgtaga gccggtcggt gttgatggca tactcgctcg agagcgagtc gagcagattc
     1081 atcaccgtgc taagttcaaa gctgatgccg acgttggtca ggctgtagct ccccttgctc
     1141 cagtcggtat tgacccactg ccgttccggg gggcactgcg gcacaacgat gaaacaggga
     1201 ttctggctct gcaggaggct gtcagcccaa accataccga ggcggtgggc cttgagcggg
     1261 gtctcgttgt ccagaccgcg ttcacccgcg ccgtgcaggg tgagaatgag gggataggtc
     1321 cggctgggat cgtacttgtc cggaacgaac agccgatacg gcagggtagt ggctttgtat
     1381 ttgtgggagc gtttgaggaa ttttgcagcc aatgcaccat cactttggga aaacagcaca
     1441 gaaacactac agaaaagccc caccgccaaa gccaccccaa aacgtctgag ttgtaccatc
     1501 aatcttcctt cagtgagtca tccggagaac ttgcatcaga aacgagcagc ctggcatcca
     1561 agtccatcct cattggtcca tataatgcgc tgcgtcgggg accaccctgg gcacgcccag
     1621 agcgattcga acgctcgacc tacggattag aagtccgttg ctctatccag ctgagctatg
     1681 ggcgcattta tactgtgtat aatttagcca tttaaagcca acaagacaag cttttttttg
     1741 gtttttaggc aaatatagat gtgttttatg cagtagatca caacagatag aatttcgcca
     1801 ggagtgtgca agaattggac atttttagcc tcagcgccgc aacagctcaa gaaaaagcgg
     1861 tcaccaggga tttttcagat ccttgctctg gtaggagtaa ttccggacga caaaataact
     1921 gcccgtattt ttgcggcgcc gctgcagcaa atccgggcgg aacaccctcg agagcagtgc
     1981 cataggcaag aggaagataa aaaacaccag gctgagcaaa accttggaga tgacaaagcc
     2041 gagtgcctgg gaaagtttgt accacagccg ggccaaggcc cgactggttt tggccatcag
     2101 caaaccgatg ccgccaagga ggaggccgat gtagagcagc agtttgatgt ccagcagcag
     2161 cgacagcagc aggcagccaa agacgagggc gagcatcgcc tcgaggcttt tttcccgagt
     2221 catgtcacaa tccttctaga aaatggtata aatgaatggt gcgaccgcac tgctgccgcc
     2281 gatgatgata aggataccca acagcaatag cacaatgatg actggcaaca accaccattt
     2341 tttccgttcc ctgagaaaat tccacagatc cattagaaaa tccacgggtt cgctcctttc
     2401 ttgcgcttcc agcagacgtt tccttcgcgg gggcctattg aacatacccg gcgggattca
     2461 tgccgtacgg ctgccggcgc ggggttctcc tcgcgccggt caatccagct tgaaggtctc
     2521 cttccagttc tccgtatcct gatagttggg ctgctcggtt ttcacgaaca ggtagtcgcc
     2581 gatgacgagg taatccatct cggtacgcat gaagcatttg taggcatcca ccggtgtgca
     2641 gatgatgggc tcgccgcgca cattaaagct ggtgttgacc agcaagccgt agccggtttt
     2701 ttccttgaag cggcgcagca gggtccagta aggctcattg gtttcccggt gcacggtctg
     2761 gatccgggct gaaaagtcga ggtgggtgat cgcgggcaca tccgagcgct gatggtacag
     2821 tctttcccag agcggcaggt catgataatg atccgggagc ggtcggcgcc ggctggagac
     2881 aacgtcggcg ataagcagca tatacgggga gggcatatcc agttcgaaga aggaggtatt
     2941 gtcttcatcg agaaccgagg gagcaaatgg acggaagctt tcgcgatact tgattttcag
     3001 attcagcttc ttctgcattt cgggattacg ggcgtcgccc aggatactgc ggttgccgag
     3061 cgcgcgcggt ccgaattcca tacggccttg catccacccc accacttgtc cggaggccag
     3121 cagatctgcc acctcgtcca cgagttgtgt aaaggaggtg tatttatggt agacggcctt
     3181 gtttttacgc gccatcagtt caatatcgat ggcggaatat tcggggccaa gaaaagcgcc
     3241 ttgcatggcg tccatggtgg gtgcaggatt tcgttcctgt ccaaaataga tatggtgagc
     3301 ggccagagcc gcacccaggg cgccgccggc atcgccggcg gcgggctgaa tgaagatgtc
     3361 gtcgaagagt ctttttttga taatcttgcc gtttgccaca cagttcagcg ccacaccacc
     3421 ggccatgcag aggtttctgg caccggtcaa ccgctgtgct tcttcagcca tcttgaggac
     3481 gatctcctcg gtgactgcct ggattgcaaa cccaagatcg cagtggtgtt gttccagggg
     3541 tgattcaggt tttctcaccg ggaagccgaa gagctggtgc catttttcct cccgggtcat
     3601 tctcaatccg gtggcatagt tgaaatagtc ctgattcaac cagatcgatc cgtcctcttt
     3661 gatatcgacg atatgttcac ggatgagtgc aatgtatttg cgggtcgcag cgccctccgg
     3721 atttccatag ggcgccaggc ccatcaactt gtactccccg gagttgacga caaatcccag
     3781 gtaataggtg aacgcggaat agagtagacc caccgagtgg ggaaaatgga gttccttgtg
     3841 aatctcgatc gtatggtccc ggccaaatcc gatcgtggag gtcgcccatt cacccacccc
     3901 atcgatcgtc aatatggcgg cctccttgaa gcgggaaggg aaaaaagcgc tggcggcatg
     3961 cgagagatga tgctcgggga aaagcatgtg cagccggtgt cggtcataca gctcgatttt
     4021 gccgagttcc tcatggatca cgcgcttgag gaacatcttt tcccgcagcc agaccggaat
     4081 ggaagccatg aacgacacca cgcccctggg ggcaaagcca taataggtct cgagcaaacg
     4141 ttcaaacttg agcaggggct tgtcgtaaaa gaccaccgca tcgagttcgt cgatggaaaa
     4201 gccggcgaaa tcgagacagt acctggcggc ctgggacgga aattcggccg tatgtttttt
     4261 ccgggtgaag cgttcttcct gagcggcggc caggatttgg ccatcctgca aaatcgccgc
     4321 cgcagaatca tgataaaaag cagagagccc cagtattttc atgccttttc cccatggtcg
     4381 tccagaatgc ccgtctgcgc cgaacgcaga gccgcagttg ttgacatttg atgtcacaga
     4441 gctgttttca ttttgttgct gccggtcgcg gcaccggctg tcattacctg gtcagcccaa
     4501 tagcagtttg catccagcat tctctgtttc ttccataaca tcattatcag gtaaaatatt
     4561 tacgtcaacg gaagaaattg taaccggatg tcaatattac cgggctaacg caacaggatc
     4621 aaggtacgcg tctgtatccc gcctgccgcc tcgagccggc acaagtaaag accgctggcc
     4681 tggccgccgc catcccaggt gaggctgtgg gctcccgccg gccagttccc tgcggcgagg
     4741 gtggccacct cccttccgct cacagaaaag aggcgcaggg agaccctagc ggggcgcggc
     4801 agggtgaagc ggatcgttgt ggactggttg aaggggttgg gatagttctg ctggagcagc
     4861 agctcgcccg gcgaggcctc ctcctcccct ccattcaccg gggcgagacg ccagagatgg
     4921 ttgttgatcc agagggtgcg gccgcggatc caagatttaa gggtggcgat ctccacctca
     4981 taggagtggg cggccgaggg ctcgggccaa cgtctgacat cccgggcccg ggcttcggcg
     5041 agggtatcgg agatctgatc gatgcgcagg tcgaggtttg cggggagcag gatggtctgg
     5101 ctcagctcgg cccagcggct cctgaccttg ccggcaaaat agggatcatg accgaggagt
     5161 tcccaccaga aggggacact gggccagtcg ccgggatgat agatgtcgtg atcgatctcc
     5221 cactcgtcca cccggccggc atcctccgca taccaggtct tgccgaaggt gaggttgaaa
     5281 tcccagatcg gcccggcgat gatcttgccc cccttgctgt cgcggtcctt gtgcatgaaa
     5341 aagctgatgc ggtaggcgtc gatgttcttg cagaactcgt tgaggataaa gtggtcgaca
     5401 aaggaatcga cgtcgatgta gcggggatag ccgaagagcg tgtccccgcg cgtgggcatg
     5461 agcatcaccg actcgaagac gttcatcacc cccctcagat agctcttctg ggcggagacc
     5521 agatcctccg ccttgggatc gtgatactgg tagtagagcc cctgcatgga ggtccatccg
     5581 gcagtctgct ctccctcctc cttgtcgaat ttccagacat agccgccggt gaccgcgtca
     5641 ccgctgatgt cggcgctctc cagttcggta acgttgaccc ggttcttgtc ctgcttgatc
     5701 ttctccatga agacatagac cccgcggtag ttgttgttga gcaccagttc gcagtacttg
     5761 acgcgggggg cataacggcc gatctcgctg gagatgcgat aggcgaggac gttgcgcatc
     5821 agcgactggt cgtcgtagga ggcatagagg atccagtcgt tctccttggg catgccgagc
     5881 agggagacgt tgcggttgtc accagcggtg gtgacggtct cgaggcggta tcctttcttc
     5941 gggtaggcca tcgaggaact gccgcgcagt tcgatatcga tgagaccgtc gtagttgttg
     6001 gggggatcgg taatggcgtt gcgtctgccc gctccgttat aaatgatctt catggtggcg
     6061 cggatgcgca ccagatcagg gatctccttg ccgcctgtat cgatgaggat gatgggcagg
     6121 ttggaatcgc gcagcgccac ccccgtggtc tgggcgggca gggcgctgag gccccccatc
     6181 aggagtagaa gtacaagggc agcccgccgt gtgcagcgct gcaatgcaga ggggacaccg
     6241 ccggggacaa tgcaattgtt catggtttgc agcatgatac agttcctgat gacggacttt
     6301 gctcccggca tggggtgacg gccgctctcc tgccgacact ggtggaggag gccgtcgtgg
     6361 agcgggcaca ggacggccta gagagtcctc gaggaattcc ggacaaccag ttcggtctcg
     6421 agcagtatcc gctcgggtgg cagcagcagc ggggattcaa tattgcggat gaggatctcc
     6481 gcggcgcggc ggccgatctc catctgcggg gcgcggatcg tcgtcagcgg caccgggtag
     6541 agcttggcat agtcgatgtc atcgttgccg atgattgaga tctcttcggg cacgcggata
     6601 ttgagttccc gcagggccga catcaccgcc agggcctgct ggtcattgaa gcagaggatt
     6661 gcggtgggat actctgttct ctccaggctt ctgaaccagg caagggcgtt gtgatagctc
     6721 tcttcatgcc gcgagccgat cgagatgatc atctcgccgg tgaaggcgag ggtgctctcg
     6781 ctgaaggcgt cgcgaaatcc ctcgatgcgc tcctgggtgt gggaggattg gggcggtccg
     6841 gcgaaatgga cgatgcggcg gtggccgcca tcgatgagat atttgaccgc ctgccggatc
     6901 gccttgagat tatcgatgac cacggcattg gcctggatgc ccttgacctc ctcgagcagg
     6961 acaaagggat agttgatcat cttgagcttg aagagatgtt caatctccgc ctggccctcg
     7021 acaatggggg cgatgatggc ccccttgata tccttggtcg agaaaagatg ggtgaacttt
     7081 ttttcacact cgtggtcgtt ttccgaactg gtgatgatca cctgataccc gtgtgcgacg
     7141 gcgtaggcgc gcactcccga ggcgatcgag gtgtaaaagg gatagttcat gtccttgatg
     7201 atgatgccta tagaacggtc ttcgctgccg ttcttgagat tcctggccat tccgcgcgga
     7261 cggaaattga gttctttcat gaccgcaaga atgcgctcgc gggtgtgcgg tttgacggaa
     7321 ttcttgccat taagtacagc tgagacggtg cccttggata ccccggcctt gctggcaaca
     7381 tcctcaatag taattctctt catatcttct ccggcctttc aggccgtctg gctccaactt
     7441 ttaaattgaa acggtttgat ttttcacgtt gaatatagtt tttttgtaaa ggaaaatcaa
     7501 ctactttctt gccttatttt catttatccc gcccggcagg tcggccgggg agactcccgc
     7561 ttgttattct tgatgccttc aaataggcgt gagccaggtt tcaggaggta tacggcgcaa
     7621 ggtgtcaaat ggaaaccgga tttcaggggg tccctcccgc tagttttttg cttgactttt
     7681 ttgcccctca tccctatatt tgaatcggtt caaatcattt aacactcctc tctgtgaggt
     7741 gctgatgaaa aatacccgcc gttacctggt aatcgcctcc ctgatctgcc ttgcccttgc
     7801 gctcctcctc tcctgtggca aaccgctgct ccggcatggc gccgaagcca cctcccatta
     7861 ccccaatctc ttcactgatc tgctcggcgt cggggagagc cttgtcacag agcggctcga
     7921 gagcgccttt cagcagctct tctacggcga cgaccgcacc cagcgcatct actacccggc
     7981 cggccccgat atggcctata tcgaggatat cctccacaac gatgtgcgca ccgaggggat
     8041 ctcttacggc atgatgatcg cggtgcagct ggacaaaaaa gccgaattcg atcgattgtg
     8101 gaaatgggcc aaaacctaca tgcagcacca ggaggggcca agcaagggct tcttcgcctg
     8161 gcattgcacg ccggcgggag aaaagctcag cgtcagcgcc gctcccgacg gcgaggagtg
     8221 gatcgtcacc gccctctttt tcgcctcgca ccgctgggga gatggtcccg gcatctataa
     8281 ctatcgggct gaagcccagg ccatcctcga tgccatgctc agcaaggagc cggcatcgga
     8341 cctccaggcg gtcatcacca acatgttcaa ccgcgaacac aggcaagtgg tctttgtgcc
     8401 caatggcgag gccgacggct ttaccgatcc ctcctaccat cttccccact actacgagct
     8461 ctgggcgcgc tgggcggaca aggagaaggc tttctggcag gatgccgccg cagtcagccg
     8521 cgagttcctt aaaaaagcag cccatcccgt aaccggcctc acccccgact atgccaactt
     8581 tgatggcacc gccactgacg ccccctgggg gggcggccat gacgccttcc gcttcgatgc
     8641 ctggcgcact gcgatgaatg tcgccgtcga ctgggtctgg ttcgcccgcg atgagtgggc
     8701 catccagcag tccaaccgcc tgctcgagtt tttcaatgac gagggtctgg cacgctacgt
     8761 caaccagtat gccctcgacg gacagccact ctcggaagaa cgcagccccg gactggtcgc
     8821 catgaacggg acagccgccc tggcagcgac cactcccctc cgcaccggtt tcgtccgggc
     8881 ggtttgggag cagcccgttc ccgacggtcc cggccgttat tacgacggac tgctccacat
     8941 gctggctctg ctccagctcg gcggccagtt ccgtatttac gcgccctgag acctgctctg
     9001 gattccggcc ggatacgctc acaccccggc cggctgttcc gcctctcccg cccggaatgc
     9061 gcaggtcccg cgctcactgt aacacgaaat cctcgaggcc atcatgatct catttcccag
     9121 ccaaaaactg ctctgcctta gcctcatgtt tggcgtgctg ctgccgttgc tgccggcggc
     9181 tgctgcactc ccctacatca cggagacgcc gcagcctggt tccttcaccc tcgccgaacc
     9241 cggccgcagc gcatacctgg tggcgagcag ccgggagtac cccggtgtgc tgcgggtggc
     9301 cggccatctg cagaacgatc tggcccgggt gaccgggagc gccccggccc ttctgcttga
     9361 tacgcctccg gcggcagggg atgtcatcct tttcggcacc ctcggcgtc
//
LOCUS       DAOPWU010000115         9384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7359_length_9384_cov_3.864723, whole genome shotgun sequence.
ACCESSION   DAOPWU010000115 DAOPWU010000000
VERSION     DAOPWU010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7359_length_9384_cov_3.864723"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PKM23_07515"
     CDS             complement(<1..597)
                     /locus_tag="PKM23_07515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNY91359.1"
                     /translation="MRILAYHMVEPRFDLAITRVTPRQFAAQIEGLLSADYALLSLRE
                     GLSSSRLQDKSVILTFDDAYASVFKHAFPVLRANGLRATLYVPAGYIGRLDDWDVNFG
                     NIQFPHMTADQILTLHAAGWEIGSHGFSHLDLTRLAGERLEEEVAGSRAVLEKLIGGR
                     VDAISYPFGNTDERVITCCLRAGYRSGVVMSRPHKTLEG"
     gene            complement(746..2866)
                     /gene="pnp"
                     /locus_tag="PKM23_07520"
     CDS             complement(746..2866)
                     /gene="pnp"
                     /locus_tag="PKM23_07520"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNY91360.1"
                     /translation="MITRKQTEFEGQQLIMETGKLAKQANGSCWVQLGDTVVLAAVVA
                     DSKASEERDFFPLTVDYREKAYAAGKIPGGFFKREGKPTDGEVLSARLIDRSIRPLFD
                     KSFRNEVQVYVWVLSADKVNDADVLGITAASLALGISNIPFTTPIAGVRVGRVEGRFI
                     ANPTFEQQEESDIDLVLSASEESIIMVEGEAREISEDDMIAALEYGHQAIRQILVLQK
                     QVVGEAGKVKMELAVAEATPDLLKAVHGYRAELAKGLRIAEKEARRQALAEINDKIKT
                     ELAESFPEKEGKISEIFHDLEKELMREMVLTEKSRLDGRGLSDIRDISIEVGLLPRAH
                     GSALFTRGQTQALASITLGTKVDEQRIEELEGEFRKTYMLHYNFPGFCTGEVKPNRGV
                     SRREVGHGNLAERAIKQVMPTEKLFPYTVRIVSDVLESNGSSSMATVCAGSLACMDAG
                     VPIKSAVAGIAMGLIKEGDQVAVLTDILGDEDHMGDMDFKVAGTREGITAFQMDIKIG
                     GISTKIMREALQRAQDGRFFILDKMQAVLEQPRPELSAYAPRIMTVKIPVDSIGMVIG
                     PGGKNIRDIVERTETTIDINDDGTVTIAAVDPVACEKAASIIRAMTAEVEVGTVYIGK
                     VKKIMKFGAFVEVLPGREGLLHVSEIEHRRIERVEDVLKVGDEVQVKVMKIDGDGKID
                     LSHKVLIERPAGAPDSNRSSFNNR"
     gene            complement(3083..4441)
                     /locus_tag="PKM23_07525"
     CDS             complement(3083..4441)
                     /locus_tag="PKM23_07525"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNY91361.1"
                     /translation="MRFRTTLILALVLALGILGVVYTDKQERNQEEADRESEKILAYS
                     GEQVLGIHLLPAGIRAERDSSGWQLLAPVHTAGDKISLDAIASLFSWAKKERIVSDDA
                     NDHASFGLIPPRAILILTSASGLDTLFIGDDSQVGSFVYARKGNSPEVFLTTTSLWSN
                     INKTLFDLRDKNILGFEQGRTDRLEIKNANGLFTMSKEGESWLLHSPKALRADARKVA
                     DLLNALQYQKALRFVEEEPHDLSAYGFGRPAIEIRVGSPASAGLRTLSIGSFDAGCYY
                     ARDAARLPVFTVDSSFVHRFNVALNDLRDKGMLRFNSEMVSAVEITAGDTTYICHRDT
                     AGSWQMLQPKTGPVRSWKMTGLISDLSSATAEGFAADEPASLRPFGLDQPRIRCRLLQ
                     KKDVIAEFLIGKNKDASTLYAMAGGGRTVYLIRSDLFDKLNTRSADLLESLPAASAAS
                     PE"
     gene            complement(4507..6039)
                     /locus_tag="PKM23_07530"
     CDS             complement(4507..6039)
                     /locus_tag="PKM23_07530"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNY91362.1"
                     /translation="MIKKIAQICGWLGLGMILAGLIVYSLTAVMAWTVYLPLILGCAL
                     LLASITLQFREIRAGLTTRSARFGSNALLMVLILLGILIVANIFAARFSWRLDTTAAR
                     QFSLAEQTRNILKSLDKDVKITGFFKSGDEAFTKELLTEYAHYSDRLRFEFSDPDKKP
                     GLAKKYQVKSYGTLIVEAEGKEERIEKSEEQLLTNAIIKVTREGKKKLYFTTGHGEKD
                     PDSDGADGLATARKALIEENYDIAKLLLARESAIPADCSMLAVAGPRTELFASEKSLI
                     MEFLQKGGKLLILLDPASPDSWSELLGEWGFKVGRDIVVDASGFGQLFGTGPTMPIVS
                     QYEEHAITRDFNLMTFFPEARSISRGENPPAGLEFREIGRSTPRSWAETSPISGQAEI
                     TFDEGKDLRGPVTLFAGAEMPAPAAAGEKAGKTRLLVFGDSDFATNSYFDTQGNGNLF
                     QNGVSWLAEEEDLISVRARSPEDRRIAITQTQSRALLYLGIIVLPLAIFIAGIAVYRN
                     RK"
     gene            complement(6036..6821)
                     /locus_tag="PKM23_07535"
     CDS             complement(6036..6821)
                     /locus_tag="PKM23_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY91363.1"
                     /translation="MKNYLAILGREWKSYFSSPIAYVVMGLFLLISGVFYYLLVTNFV
                     QMCMQADMQARYYQMAVPKLNVNMMAIRPLLHNMSLFALFFLPLITMRLFSDEKKSGT
                     IELLLTSPVTNTQSILGKFSAAAGLYLLMLLLSGLYMAVLFFLGKPEIGQILTGYLGL
                     FLLGLCYIAFGLFFSTLTDNQIIAAVSTVAFILIFWAIGWISGMASPAVGKILGNFSL
                     IEHFDDFAKGVLDTRHIVFYISFTVMGLFLSFVSIESAKWRGR"
     gene            complement(6818..7774)
                     /locus_tag="PKM23_07540"
     CDS             complement(6818..7774)
                     /locus_tag="PKM23_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY91364.1"
                     /translation="MIEVKQVTRYFGRVMAVDHVSFQVEKGEILGFLGPNAAGKTTTM
                     RILTTYLPATSGTASVAVFDVHQDSMQVRKRIGYLPENPPLYTEMRVADYLEFVARIK
                     GVDPRTRKHAIAETMDKTGISAVSRRVIKQLSKGFKQRVGLAQALVHDPDVLILDEPT
                     VGLDPKQIIEVRELIRSLAGDHTIILSTHILPEVSMTCERVVIIDQGRVVAEDTPDNL
                     TRKLAGSQRFTLIAGGPAGGILELLNKVEGVRQVKLLETGAPAQTHQFHIEGDAEGSI
                     NPAIARAIVEKGWDLFELRPEGLSLEDVFLRVTTREEEGGGQ"
     gene            complement(7858..8127)
                     /gene="rpsO"
                     /locus_tag="PKM23_07545"
     CDS             complement(7858..8127)
                     /gene="rpsO"
                     /locus_tag="PKM23_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNY91365.1"
                     /translation="MPLTKEVKLELVEKYGKRPDNTGSTEAQIAMLTERINQLTKHFH
                     TNPKDFHSRRGLLKMVGRRRRLLDYLKANDISLYRSLIQELGIRR"
     gene            complement(8141..9037)
                     /locus_tag="PKM23_07550"
     CDS             complement(8141..9037)
                     /locus_tag="PKM23_07550"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNY91366.1"
                     /translation="MVTLGTFDGVHLGHQTILAELKTRANNLQARATMVTFEPHPQLV
                     LNNPSRPPHEVLTTIEEKIEILNQLGLDRLVVANFSPAFAGLEPERFVTEILLERLKM
                     REIIIGHDHAFGRARSGNLELLERMGAEQSFAVASLPPYRVEDEIVSSTKIRHYLQKG
                     LMEKATAMLGRSYSINGLVVHGEGRGRGMGFPTINLRPFSPYKLVPRAGIYASRCHLD
                     GKSYAAATYIGKKPTFAAEERVIETYIIDFDGQLYGQEVRVDFISFIREDTRFASTNA
                     LVEQIKQDISKSVEVLASQSSY"
     gene            complement(9076..>9384)
                     /locus_tag="PKM23_07555"
     CDS             complement(9076..>9384)
                     /locus_tag="PKM23_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNY91367.1"
                     /translation="QRSYKLARAGQLKSPEPRRVTVFSLKVARVSENSLDFTVVCSKG
                     TYIRALARDLALALGTVGFLSRLTRTRIGPHTLQEAIEVTALPQMFNKNSLEHLYSCR
                     "
BASE COUNT         1971 a         2534 c         2845 g         2034 t
ORIGIN      
        1 accttcaagt gttttgtgag gtctggacat caccactccg ctgcgatatc cggccctcag
       61 gcagcaggta ataacccgtt catccgtgtt gccaaagggg taggaaattg cgtcgaccct
      121 cccgccaata agtttttcga gtactgccct ggatcccgcc acctcctctt ccagcctctc
      181 tcctgccagc cgtgtcaaat ccagatgact gaagccgtgg gagccgatct cccacccggc
      241 cgcatgcagg gtgagaatct gatcagctgt catgtgggga aactggatat tgccgaaatt
      301 gacatcccag tcgtcgagtc ggccgatata tcccgccggg acatagaggg tggccctcag
      361 gccattggcg cgcagcaccg ggaaggcgtg cttaaaaaca gaggcgtagg cgtcgtcaaa
      421 ggtgaggata acgctcttat cttgcagcct ggagctgctg aggccttccc tgagtgagag
      481 cagggcataa tccgcactga gcagtccttc gatctgagca gcgaattggc gcggggtgac
      541 ccttgtgatg gcaaggtcaa accgcggttc aaccatgtga taggcgagaa tgcgcacgac
      601 ggatttgtat aaagtggatg cgaggtcttt gcggcatgtg tcttcacagc aaaacaagga
      661 tgccgcggga aaagaaaaaa taaagagcct ccgctccata aagcggaggc tctgattata
      721 aatgggtcga cttgcagccg cttaatcagc ggttgttgaa ggaggagcgg ttcgagtccg
      781 gggctccggc cgggcgctcg atcagcacct tgtggctgag gtcgatcttg ccgtcgccgt
      841 cgattttcat caccttgacc tgcacctcgt cgccgacctt gagcacgtcc tcgacgcgtt
      901 caatgcggcg gtgttcgatc tctgagacgt gcagcaaccc ttcacgtccg ggcagcacct
      961 cgacgaaggc tccgaacttc atgatcttct taactttgcc aatgtagacc gtgcctacct
     1021 caacctcggc ggtcatcgcc ctgatgatgc tggcggcctt ttcgcaggcc accggatcaa
     1081 ccgcagcaat ggtaactgtg ccgtcgtcat tgatatcgat agtggtctcg gtgcgctcga
     1141 cgatgtcgcg gatgtttttg ccacccggtc cgatcaccat gccgatagaa tcgaccggaa
     1201 tcttgacggt cataatgcgc ggggcatagg cggagagttc tggtctcggc tgctcaagca
     1261 cggcctgcat cttgtcgagg atgaagaagc gtccatcctg ggcgcgctgc agggcttcgc
     1321 gcatgatctt ggtggaaatc ccgccgatct tgatgtccat ctggaaggcg gtaatgcctt
     1381 cgcgggtgcc ggcaacctta aagtccatgt cccccatgtg atcctcgtcg ccgagaatat
     1441 cggtcagaac cgcaacctga tcgccctcct tgatcagacc catggcgatg ccggcgacag
     1501 cgctcttgat ggggacgccg gcgtccatgc aggcgagaga gcccgcgcag accgtggcca
     1561 tcgaagaaga gccattggac tcgagcacat ccgaaacgat gcgcacggta tagggaaaga
     1621 gtttttccgt cggcatcacc tgcttgatag cgcgctcagc gagattgcca tggccgactt
     1681 cccggcggct gacaccgcgg ttcggcttga cctcacccgt gcaaaagccg ggaaaattgt
     1741 aatgcagcat ataggtcttg cgaaactccc cttcaagctc ctcgatgcgc tgctcgtcca
     1801 ctttggtgcc gagggtgatg gaagccaggg cctgggtctg gccgcgggtg aagagtgccg
     1861 aaccatgggc gcggggcagc agtccaacct cgatggagat gtcgcggata tcggagaggc
     1921 cgcggccgtc gaggcggctt ttttctgtca gaaccatctc gcgcatcagc tctttttcca
     1981 ggtcgtgaaa gatttcgctg attttcccct ctttttcggg aaaactctcg gccagttcag
     2041 tttttatctt gtcgttgatt tcagcaaggg cctgccggcg ggcttccttc tcggcgatgc
     2101 gcagtccctt ggccagctca gcccggtagc cgtggacagc cttcaggaga tcgggagtgg
     2161 cttcagccac ggcaagctcc atcttgactt taccagcctc gccaaccacc tgcttctgca
     2221 gaacaaggat ttgacggatg gcctggtggc cgtattcgag ggcggcgatc atgtcatctt
     2281 ccgaaatctc gcgcgcctca ccttcgacca taatgatcga ttcttcggag gcggacagga
     2341 ccagatcgat gtcgctctcc tcctgctgtt cgaaggtggg gttggcgatg aagcggccct
     2401 cgacacggcc gacgcgcacg ccggcgatgg gcgtagtgaa gggaatattg gagattccca
     2461 gtgccaggga tgcggcagtg attccgagaa catcggcgtc gttgaccttg tccgccgaga
     2521 gcacccagac atagacctgc acctcattgc ggaagctctt gtcgaaaagc ggccggatgc
     2581 tgcggtcgat caggcgggcg ctgagcacct ctccgtcggt aggtttgcct tcacgcttga
     2641 aaaaaccgcc cgggatcttg ccggcggcat aggctttttc gcggtaatcg acggtgagcg
     2701 ggaagaaatc acgctcctcg gaagccttgg aatcggccac gacggcggcg agcacgacag
     2761 tgtcgccgag ctgaacccag caggagccgt tggcttgctt ggccaacttg ccggtttcca
     2821 taatcagctg ctggccttcg aattctgttt gtttgcgagt aatcatttgt cctccaaaac
     2881 gaccaggtct ctgccccgaa gatgaaaatg cgaggacaat gtctttggca ggcatacgcg
     2941 ctgcgcaaat tcatgttggc gtggtgtcaa cctgaatcag tgtgtgtgaa atacggggtt
     3001 ttggccccac ttcagcgcag ccgattcatt gctcgcggcc atcagccgcg tggaacaaac
     3061 cggctgcgtc tgtcttgtaa cgctattccg gtgaagcggc cgatgctgcc ggaagggatt
     3121 ccagcagatc agccgatctt gtattcaatt tatcaaagag atcgctcctg atcagataaa
     3181 ccgttcttcc gcctccggcc atggcataaa gtgttgaggc gtccttgttt tttccgatca
     3241 gaaactcagc gatgacatct tttttctgca gcagccggca gcgaatccgg ggctggtcga
     3301 ggccgaaggg ccgcagcgag gcgggttcgt cggcagcgaa cccctcggcc gtggcgctgg
     3361 agaggtcgct gatcagcccg gtcattttcc aactgcgcac cggcccggtt ttgggctgga
     3421 gcatttgcca gctgccggcg gtatccctgt ggcagatata ggtggtatct ccggcggtga
     3481 tttcgacagc gctgaccatc tcgctgttga accggagcat gcccttgtcc cgcaggtcgt
     3541 tcaatgcgac attaaacctg tgaacaaagg aggagtcgac ggtgaaaacg ggcagtctgg
     3601 cggcatcacg cgcatagtag cagcctgcgt caaatgagcc gatggagaga gtgcgaaggc
     3661 cggcggaggc cggcgaaccg accctgatct cgatggccgg gcgaccgaaa ccgtaagcgg
     3721 agagatcgtg gggctcttcc tcgacaaatc gcaaggcttt ctggtactgg agggcattga
     3781 gcaggtcggc aaccttgcgg gcgtcagccc ggagggcttt cggagagtgc aaaagccagg
     3841 attccccctc tttgctcata gtaaacaagc cattcgcgtt tttaatttcc agccggtcgg
     3901 tacggccctg ctcgaagccg aggatgtttt tatcacgcaa gtcaaaaaga gttttgttga
     3961 tgttgctcca caggctggtc gtggtcagaa aaacctcggg actattccct ttgcgggcat
     4021 agacgaacga gccgacctgg ctgtcatccc cgatgaagag ggtgtcgagg ccggaagcgc
     4081 tggtgaggat gaggatggcg cgcggaggga tcaagccgaa ggaggcatgg tcgttggcat
     4141 cgtcggaaac gatgcgttcc tttttagccc agctgaagag cgaggcgatg gcatcgaggc
     4201 tgattttatc gccggcagtg tggaccggag ccagcagctg ccagccactg ctgtcgcgtt
     4261 ctgcccggat accggcgggc aggaggtgga ttccaagcac ctgctcccca gaataggcga
     4321 ggattttttc actctcccga tcggcctcct cctggttcct ctcctgcttg tccgtataaa
     4381 caacgccaag gatgcccaga gcgaggacaa gcgcaagaat aagcgttgtt ctgaatctca
     4441 ttgcagaaaa ctcctgttat gtaacccggc ctctctgcgc gggagaggcc gtcacatttc
     4501 tgtagatcac ttccggttgc ggtagacggc aatgccggcg atgaatattg ccagtggcag
     4561 cacgatgatg ccgagataga gcagcgcccg cgactgggtt tgggtgatgg cgatgcggcg
     4621 atcctccgga ctgcgggcgc gtaccgagat cagatcttcc tcctcagcga gccagctgac
     4681 gccattctgg aaaagattgc cgtttccctg ggtgtcaaag taagaattgg tggcgaaatc
     4741 agaatcgccg aagacaagca ggcgtgtctt tcccgccttc tcccctgctg cagcaggggc
     4801 gggcatttcg gcgccggcga agagggtgac aggaccgcgc aggtccttgc cttcatcgaa
     4861 ggtaatttcc gcctgaccgc tgatggggct ggtttcggcc cagctgcgcg gtgtgctcct
     4921 gccgatctcc ctgaactcaa gcccggctgg gggattctct ccgcggctga tcgaacgggc
     4981 ttcggggaag aaagtcatga ggttgaagtc acgggtgatg gcgtgttcct cgtactgcga
     5041 aacaatgggc atggtgggac cagtgccgaa gagttggcca aatcccgagg catcaaccac
     5101 gatatcgcgg ccgaccttga agccccattc gccaagcagc tccgaccagg agtcggggga
     5161 agccggatcg agcaagatca ggagctttcc gcccttctgg agaaactcca taatcaggct
     5221 cttttcgctg gcgaacagtt cggtgcgcgg cccggcgaca gcaagcatgc tgcagtcggc
     5281 ggggatggcg ctctcccggg caaggaggag cttggcgatg tcgtaattct cctcgatcag
     5341 ggctttgcgg gcagtcgcaa ggccatcggc gccatcactg tcgggatctt tctcgccatg
     5401 gccggtggtg aaatagagct tctttttgcc ctcacgggtc accttgatga tggcattggt
     5461 gagcagctgc tcttcgctct tctcgatgcg ctcttccttg ccttcggcct cgacgatcag
     5521 ggtgccatag gacttgacct ggtatttttt tgcgaggccg ggctttttgt cgggatcgct
     5581 gaattcgaag cgcaggcgat cggaatagtg agcgtattca gtgagcagtt ctttcgtgaa
     5641 ggcttcatcg cccgatttga aaaagccggt aatttttaca tctttatcaa ggcttttcaa
     5701 tatgttgcgg gtctgttccg caaggctgaa ctgtctggct gcagtggtat cgaggcgcca
     5761 ggaaaatcgc gcggcaaaga tattggctac aatcagaatg ccgagcagga tcagcaccat
     5821 cagcagggcg ttgctgccaa acctggccga gcgcgtggtg agtccagccc tgatctcgcg
     5881 gaattgcagg gttatagatg cgaggagtag agcacagccg agaataaggg gtagatagac
     5941 tgtccatgcc atgacggcgg taagcgaata gacgatcagg ccagcgagga tcatgcccag
     6001 accaagccaa ccgcagattt gtgcgatttt ttttatcatc ggcccctcca tttggctgat
     6061 tcaatggata cgaaagagag gaagagtccc atgacggtga aactgatata aaaaacgatg
     6121 tggcgtgtat cgagcacgcc cttggcaaag tcgtcaaagt gctcgatcag cgaaaagttg
     6181 ccgaggatct tgcccacagc cggactggcc atgcccgaaa tccagccgat cgcccagaag
     6241 atcaggatga aagcgacggt gctcactgcc gcgatgatct gattgtcggt cagggtggaa
     6301 aaaaagagac cgaaggcgat ataacagagt ccgagcagaa agaggccgag gtagccggtg
     6361 agaatctggc cgatttcggg cttgccgagg aaaaagagaa ccgccatata gaggccggac
     6421 aggagcagca tgagcagata gaggccggcg gcggcgctga atttgccgag aatcgattgg
     6481 gtattggtca ccggcgaggt gagcaatagc tcgatcgtac cactcttctt ctcgtcggaa
     6541 aagagacgca tcgtgatgag cggcagaaaa aagagagcaa aaaggctcat attgtgcagg
     6601 agcggacgga tggccatcat gttgacattg agtttgggca cagccatctg gtaataacgc
     6661 gcctgcatat ccgcctgcat gcacatctgc acaaaattgg tcaccagcag atagtaaaaa
     6721 acgccggaga taagcaaaaa cagccccatc accacatagg cgatcggcga gctgaaatag
     6781 gacttccact cccggccgag gatagcgaga tagtttttca ttgtccacct ccttcctctt
     6841 cccgggtggt cacacgcaga aaaacatcct cgaggctgag gccttcgggc cgcagttcaa
     6901 acagatccca gcccttttcc acaatggcgc gggcgatggc gggattgatg ctgccttcgg
     6961 catcaccttc gatgtgaaac tggtgggtct gcgcaggtgc tccagtctcg aggagcttga
     7021 cctggcggac accctctact ttgttgagga gttccaggat gcctcccgcc gggccaccgg
     7081 ctatcaatgt gaagcgctgg gatccagcca gttttcgggt gaggttgtcc ggggtatcct
     7141 ccgcgacgac acggccctgg tcaatgatga ccacgcgttc acaggtcatg ctgacctcag
     7201 gaaggatgtg ggtgctgaga atgatggtgt ggtcgccggc gaggctgcgg atgagctcgc
     7261 gaacttcgat aatctgtttg ggatcgagtc cgacagtggg ttcgtcgagg atgaggacgt
     7321 cgggatcatg gacgagggcc tgggcgaggc cgacgcgctg cttgaatccc ttggagagct
     7381 gcttgataac ccggcggctg acggcgctga tgccggtttt gtccatggtc tcagcgatgg
     7441 cgtgcttgcg ggtgcgggga tcgacaccct tgatgcgggc gacgaactcc agataatcgg
     7501 caacgcgcat ttccgtgtaa agtggcggat tctccggcag ataaccgatt cgcttgcgaa
     7561 cctgcattga gtcctgatgg acatcaaata cggccaccga ggcggtgcct gaggtggccg
     7621 gcagataggt ggtcaggatg cgcatggtcg tcgtctttcc ggcagcgttg gggccgagaa
     7681 atccgaggat ttcgcccttc tcgacctgaa acgagacatg atccaccgcc atgacgcgac
     7741 cgaagtaacg cgttacttgt ttcacttcaa tcattgttgg gagcctcctt tcttaatggc
     7801 cgattccgcg ccgctgaagt gagtgatcag tcgcacctga aaaaagagcc cgaatcctta
     7861 ccggcggatg ccgagttcct gaatcaggga gcgatagaga gagatatcgt tggccttgag
     7921 ataatcgagc agcctgcggc ggcgtccaac catcttgagc agtccacgcc gggagtggaa
     7981 atccttgggg ttggtgtgga agtgcttggt cagctggtta atgcgttccg tgagcatggc
     8041 aatctgggcc tcagtgctgc ccgtattgtc aggacgcttg ccgtactttt cgacgagttc
     8101 cagtttcact tctttggtca aaggcatgaa caacctccgg ttaataactt gactgactgg
     8161 ctaatacttc aacagattta ctaatgtcct gtttgatctg ttcaaccagg gcgttggttg
     8221 aggcaaatcg agtatcttcc cgaataaagg agatgaaatc gaccctgacc tcctgtccat
     8281 agagctggcc gtcgaaatcg atgatgtagg tttcgatcac ccgctcctcg gcggcaaagg
     8341 tcggtttttt gccgatatag gtggcggcgg cgtagctttt gccgtcgaga tggcagcggg
     8401 aggcatagat gcctgcgcgt ggcaccagtt tgtagggcga aaagggacgc aggttgatgg
     8461 tcgggaaacc catacccctc cctctaccct ctccgtgcac gaccaggccg ttaatgctgt
     8521 aggaacgacc gagcatggcg gtggccttct ccatcagccc cttttgcagg tagtggcgga
     8581 tcttggtgct tgagacgatc tcgtcctcca cacgataggg gggcaatgaa gcgacggcga
     8641 agctctgttc ggcgcccatg cgctccagca gttcgaggtt accgcttcgc gccctgccaa
     8701 aagcatggtc atggccgatg atgatctcgc gcatcttcag ccgctcgagc aggatttccg
     8761 ttacaaagcg ctcgggctca agtccggcga aggcggggga aaaattggcc accaccaggc
     8821 ggtcaaggcc gagctggttg agaatctcaa tcttctcctc aatggtcgtc agcacctcat
     8881 ggggcggacg agagggatta ttgagcacca gctgcgggtg gggctcaaag gtgaccatag
     8941 tcgcccgggc ctgcaggtta tttgcgcggg tctttaactc tgcaaggatc gtctgatggc
     9001 caaggtgcac gccgtcgaag gtgcccagtg tgaccacact gctctccgca ggcggaagtg
     9061 tattaatgtt ccagattatc tgcatgaata caagtgttcc agcgaattct tattgaacat
     9121 ctgcggcagc gcggtcacct cgatggcctc ctgaagagta tgggggccga tccgtgttcg
     9181 ggtcaggcgg ctgagaaagc ccaccgttcc cagggcgagg gccagatccc gcgccagggc
     9241 gcggatgtaa gtccctttgg aacagacgac ggtgaaatca agactgtttt ccgatacccg
     9301 cgcgaccttc aggctgaaaa ccgtgaccct gcggggttca gggcttttca gttgaccggc
     9361 ccgggccagt ttgtaggagc gctg
//
LOCUS       DAOPWU010000116         9367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7381_length_9367_cov_5.074205, whole genome shotgun sequence.
ACCESSION   DAOPWU010000116 DAOPWU010000000
VERSION     DAOPWU010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7381_length_9367_cov_5.074205"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PKM23_07560"
     CDS             <1..594
                     /locus_tag="PKM23_07560"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNY91368.1"
                     /translation="VIRALTTRQERMIRMSYHQTEIPKLVEIAFPIVITLIAGIVAPI
                     SVALIGSLMFGNLIRACGVLERLSNAAQNELANLVTLMLGITIGSTMVAQDFLRADTL
                     IIMGMGLIAFIFDTAGGVLFAKFLNLFLKHKVNPMVGAAGISAFPMSARVIQRMAKEE
                     DPTNFLLMQSTSANVAGQLGSIVAGGLLLALVPLFMK"
     gene            608..748
                     /locus_tag="PKM23_07565"
     CDS             608..748
                     /locus_tag="PKM23_07565"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNY91369.1"
                     /translation="MNPEIDAALKIMGLGLGGVFTVLILFYFVTRLLIVIARKLPPME
                     EK"
     gene            complement(823..2715)
                     /locus_tag="PKM23_07570"
     CDS             complement(823..2715)
                     /locus_tag="PKM23_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY91370.1"
                     /translation="MNRRTSSRAWGSLAGLFALLPLLINAQILAAQSPRVSGVVSTSG
                     TSVANASVTFIDAADSTVRFDTLTDEFGGYLLSLSTGVESDAASPAAFRLEQNYPNPF
                     SSSTAISWFLERPDEALLIIYDLVGREVRRFTHGPQPSGVHRLLWDGRDERGEQLPRG
                     VYFCRLQSSNRIMTRKMLYLPGQNLENNSGPAIPLHPARTAAGPGLENVRLSDSWIVR
                     ITNTANTIPRIVPAEFSGITVEGITVRNFYVDRHQPALTAVVQFDQPRQIIRGFGAAN
                     IVGWRPDMTAGQIATAFGTGDGQLGFSILRLRVPPDSTAFKAQVATARAAHQRGAILI
                     ASPWTPPARMKTNNSLIGGRLRLDCYDDFARHLASFVNYMAAQGAPLYAISVQNEPDV
                     NVTYESCDYNGEEMRKFMRENAPAITTRVMAPEGCNFNRSLSDPILNDPVATANLGMI
                     CGHLYGGGLEPYPLAREKGVEVWMTEHLVLDTDWASALATGKEIHDCMAAEMNAYVWW
                     YIIRYYGPIDENGTVTRRGWVMSHFSRFIRPGCRRIEVTENPQPGVHLSAYTEGTNLV
                     IVALNTGARAAEQPFALSGSSVTALTPFVTTAEKNCIAGGAISVSDGRFTLTLEPSSI
                     TTVVSR"
     gene            complement(2855..3871)
                     /locus_tag="PKM23_07575"
     CDS             complement(2855..3871)
                     /locus_tag="PKM23_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HNY91371.1"
                     /translation="MNLEQEKYAHFALVREMMDAIHLVEEFDPARSAAVLAAIQKTGR
                     LLLTGEGSSRIFPAKNAVRKALTLGLDLAVATEGSRQAAGYDLSRYAVFCASNSGKTR
                     EVILLAQKLAAEGNPNRFALCAHHGTPLEQTCVRTFVLNCGGEKAVAATKSVVEQALF
                     YDSLLWQMGSAPQAGWQRKLADAMRTALTMPIDPAIIAAAAKAPTIYFAGYNDGVAEE
                     LTLKTNEITRKKSDFLEGTYAVHGIEEVMDPADVLFVIDPIASETAKLQEALVKGVGL
                     TVIAISAQETPFPTIRVPEAGALNPYLFLTAGWNLLVEIGLALGIDLDKPQRARKVGN
                     EFTG"
     gene            complement(3904..6108)
                     /locus_tag="PKM23_07580"
     CDS             complement(3904..6108)
                     /locus_tag="PKM23_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HNY91372.1"
                     /translation="MDTVSILPAGSIGKNFIQAPWLPEGKDEYEYRNLQTLWPLERWR
                     HLHAVEVETLVKNNNTSSSWDDILVTDVFDPSLIANNQFFGLVRIGRLQKSVLEHNDL
                     RLPTGITGSIIIACDIGDDVAIHNVGYLSHFIIGNRCILSKVDEMNTSDHAKFGNGIV
                     KEGEREEVRVWLDLMNEAGCRRVAPFDGMIAADAWLWAKYRDDRPLQARLKEMTQISM
                     DAHRGYYGLIEDQCVIKNSLVLKDVKIGAHCYIKGANKLKNLTINSSQAEPTQIGEGV
                     ELVNGIIGYGCHIFYGCKAVRFVLGDNSNLKYGARLINSFLGDNSTISCCEVLNTLIF
                     PAHEQHHNNSFLVAGVVMGQSNIAAGATIGSNHNSRANDNEIQAGRGFWPGLCTSVKH
                     SCRFASFVLISKADYPAELDIPLPFSLLNNNATLNRLEIMPAFWWMYNMYALARNGAK
                     YRSRDKRKNKKQNIEFECLAPDTIEEIFAACRLLEIWTARAWLRSAGRPEDGVSEQAL
                     AARGRDLLSGPEEEVAHLEILGENMERSSRKVVILKTWQAWRAYHDMIRYYGVKNLAA
                     WIHAHPEAGFEAMQKALSGRRIERWVNFGGQLMHEKETDRLRADIVSGRLRDWQAIHR
                     RYTSLWEKYPLAKQKHAFASLCSVLGVRRLNRKQWLAALEDSVRIQQFVSDQVYLSRK
                     KDYDNPFHRATFRNDEEMAAAIGTIDENDFIRQVREESAEYLRLVAEIKQRS"
     gene            complement(6184..6687)
                     /locus_tag="PKM23_07585"
     CDS             complement(6184..6687)
                     /locus_tag="PKM23_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3996 domain-containing protein"
                     /protein_id="HNY91373.1"
                     /translation="MKMKKVLLIAVLMLAFASGSYANGIGLQYNLNAGELYTNGIAIN
                     MKMDDQPLVLALSYYLGDQKTLGLTADYWLFNNRITVTGNSDVNWFAGVGAFLSTRFD
                     DDDKTKEDDKGMKGGVRIPLGVNMMIDKKIEPFFQVVPSIGLHFSPNIGATHLYFPMS
                     IGFRIWF"
     gene            complement(6886..7770)
                     /locus_tag="PKM23_07590"
     CDS             complement(6886..7770)
                     /locus_tag="PKM23_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNY91374.1"
                     /translation="MKLLMLSNPLAGRRRGLAVAEKSLEMLRQRGHTVENIFSDRPGH
                     LVEVAAKEVNGAWDGIIALGGDGTLFEVINGMMKGNPELPIPLGVLPAGTGNSFSRDL
                     GIGTHADAIELIAAGATRPVDLGECRSDDTSFVFINILGFGFVADVAQKAYSYRKWGA
                     LNYVIGVFIITRNLKSYPLEFEIDGRSFQRDNIFVEISNSRKTGGDMIMAPDASIDDG
                     LLDVVILNKLSRLRLLSALPRIFAGTHLKMKEVEHFTASTMSFRSTPAKVLTPDGEIT
                     GTTPITVNVLPGKIRVFG"
     gene            8004..>9367
                     /locus_tag="PKM23_07595"
     CDS             8004..>9367
                     /locus_tag="PKM23_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNY91375.1"
                     /translation="MEMTRVDRRRAQAAAPEIDHQQVSWRDLRESANRAFSRAAGAPL
                     IPGNRIRLLKDAAENYPAWLEAIAAARKHIHLESYIIYGDATGRRFADALLQKAAEGV
                     CVRVIYDWLGGFGKTPHKFWERLRTGGIQVRCFNRPRLDRPLGWLSRDHRKMLAVDGQ
                     VGYVSGLCIGMAWEGLPAKKIEPWRDTGVEVHGPAVADIEKAFAQVWAMTGEELPDGE
                     EGAGGIIAPAGTASLRVVANIPATAGLFRLDQLVAALARERLWLTDAYYAGNTPYVQA
                     LRSAAVDGVDVRLLVPNATDIPVVKALSRAGYRTLLESGVRVFEWNGTMLHAKTALAD
                     RHWARVGSSNLNISSWLGNCELDVVVEDEHFAAGMEAMYLADLDNATEVVLSRKQRVL
                     APGEPPHPARTITSGSGSAGRAAAGAVRIGHAVGAAFTGRRLLGQIEARLMTLIGVLF
                     LLLAA"
BASE COUNT         1710 a         2681 c         3030 g         1946 t
ORIGIN      
        1 gtgatcaggg cgctgacgac ccggcaggag cggatgatcc gcatgagcta ccaccagacc
       61 gaaattccca agctggtgga gatcgcattt cccatcgtca tcaccctcat cgccggcatc
      121 gtcgccccca tcagtgtcgc cctgatcggc tcgctgatgt tcggcaacct catccgcgcc
      181 tgcggcgtgc tcgagcgcct ctccaacgcc gctcagaacg aactcgccaa tctggtcacc
      241 ctcatgcttg gcatcaccat cggctcgacc atggtcgcgc aggatttcct ccgcgccgat
      301 accctgatca tcatgggcat ggggctgatc gccttcattt tcgacaccgc cggcggggta
      361 ctcttcgcca agtttctcaa cctgtttctc aaacacaagg tcaatccgat ggtcggtgcc
      421 gccggcatct cggcctttcc catgtccgcg cgggttatcc agcgcatggc caaggaggag
      481 gatccgacca actttcttct gatgcagtcc accagcgcca atgtggcggg ccaactcggc
      541 tcgatcgtcg ccgggggact gctcctcgcc ctggttcctt tgttcatgaa atagtgaggt
      601 tgctgatatg aatccggaaa tcgatgctgc tctcaagatc atggggctcg gactcggcgg
      661 ggtcttcacc gtcctgattc ttttctactt tgtcacccgg ctgctgattg ttatagcccg
      721 caagctgccc cccatggaag aaaaatagcc ccgctattgc gctccaataa aaaaggccct
      781 gtactgcagg gcctttgctt tttacctcaa gaggttgaat gatcatctgg agaccacggt
      841 agtgatgctc gagggctcca gagtgagggt gaaccggcca tcgctcacgc tgatcgcccc
      901 gcccgcgatg cagttttttt cagcagtagt gacaaagggc gtcagggctg tgacggagct
      961 tccggagagg gcgaagggct gttcagcggc tctcgccccc gtattgaggg cgacgatgac
     1021 aaggttggtc ccctcagtgt aggcgctgag gtgcaccccc ggctggggat tctccgtaac
     1081 ctcgatgcgc cggcagccgg gccgtatgaa gcgtgaaaaa tgggacatga cccaaccgcg
     1141 ccgggttacg gtgccgtttt cgtcgatggg gccgtaatag cgaatgatgt accaccagac
     1201 gtaggcgttc atctccgcgg ccatgcagtc gtggatctcc ttgccagtgg cgagcgcgct
     1261 cgcccagtcg gtatcgagca ccagatgctc ggtcatccag acctcgacgc ccttttcgcg
     1321 ggcgagggga tagggttcca ggccgccgcc gtagaggtgg ccgcagatca tgcccaggtt
     1381 ggcggtggcg acggggtcat tgaggatcgg atcggagagg ctgcggttaa agttgcagcc
     1441 ctccggggcc atcacccggg tggtgatggc gggggcattc tcccgcatga atttgcgcat
     1501 ctcctcaccg ttatagtcgc aggactcata ggtgacattg acatcgggct cgttctgcac
     1561 cgagatggcg tagaggggcg ccccctgggc cgccatgtaa ttgacgaagg aggcgagatg
     1621 gcgggcgaaa tcgtcatagc aatccagccg cagccggccg ccgatgaggc tgttgttggt
     1681 tttcatgcgg gccggcgggg tccagggcga tgctatgagg atcgccccgc gctggtgggc
     1741 agcccgggcg gtggccacct gcgccttgaa ggcggtcgaa tcggggggga cgcgcaggcg
     1801 cagaatggag aagccgagtt ggccgtcgcc ggtgccgaag gcggtggcga tctggcctgc
     1861 ggtcatgtcc ggccgccagc cgacgatatt ggccgctccg aaaccgcgaa tgatctgccg
     1921 gggctggtcg aactgcacga cagcagtcag ggcgggctga tgcctgtcga cataaaaatt
     1981 cctgactgtt atgccttcga cggtgatccc ggagaattcc gcgggcacga tcctggggat
     2041 ggtattagcg gtattagtga tgcggacaat ccaggagtcg ctgaggcgga cgttctccag
     2101 acccgggcct gcggctgtgc gcgctggatg gagcgggatt gccggcccgc tgttgttctc
     2161 caggttctgg cccggcaggt agagcatctt tctggtcatg atccgattgc tgctctgcag
     2221 ccggcagaaa tagacaccgc gagggagctg ctctccccgt tcgtcgcggc cgtcccagag
     2281 caggcggtga acgccgctgg gctgtggccc gtgagtgaat ctcctgacct cccggcccac
     2341 gaggtcatag ataatcagca gggcctcatc cggccgctcc aggaaccagg agatagcggt
     2401 cgaggacgaa aagggattgg gataattttg ctcaagcctg aaggcggcag gcgaggccgc
     2461 atctgattcg acgccggtgg agagcgagag gagatagccg ccgaattcat cggtcagcgt
     2521 gtcaaagcgg acagtggaat cagccgcatc gataaaggtc accgaggcgt tggctacgga
     2581 tgtgccggag gtagagacca caccgctgac ccgcggtgac tgcgcggcca ggatttgggc
     2641 gttgatgaga agcgggagga gcgcaaatag ccccgcgagc gatccccatg cccgtgatga
     2701 agtgcgtctg ttcatactat acctttcaaa cattccagga aaacatctcc ccggcacaat
     2761 ttaacgaaat gagcgcaagg atgcaagctt ttgacgcccg ctgaaaccat ctgcaataca
     2821 tccggccggt ccgcccacct cttcgaacct gtcctcaccc ggtgaactcg ttgcccacct
     2881 tgcgcgcgcg ctgcggcttg tcgaggtcga tgccgagggc aaggccgatc tccacgagca
     2941 ggttccagcc ggcggtgagg aagaggtagg gattgagcgc cccggcctcc ggcacgcgga
     3001 tggtcggaaa gggggtctcc tgcgccgaga tggcgatcac ggttagccct acccccttga
     3061 ccagggcctc ctgcaatttg gcggtctcgg aggcgatcgg atcgatgaca aagagcacat
     3121 cggccggatc catcacctcc tcgatgccgt ggacggcgta ggtgccctcg agaaagtccg
     3181 attttttgcg cgtgatctcg ttggttttga gggtgagctc ctcggccacg ccgtcgttgt
     3241 agccggcgaa atagatcgtc ggggctttgg ccgcagcggc gatgatggca gggtcgatgg
     3301 gcatggtcag cgccgtccgc atcgcatcag ccagtttcct ctgccaaccc gcctgcgggg
     3361 cggagcccat ttgccagagc aaggagtcgt aaaagagagc ctgctcgacc acgctcttgg
     3421 tcgcggcgac ggccttttcc cctccgcagt tgagcacaaa ggtccgcaca caggtctgtt
     3481 cgaggggggt gccatggtgg gcgcagaggg caaagcggtt gggattgcct tcagccgcca
     3541 gtttttgcgc gagcaaaatg acctctctgg tcttgcctga attggaggcg caaaagaccg
     3601 cataacggga gaggtcgtag ccggcggcct ggcgggatcc ctcggtcgcc acggcaaggt
     3661 cgaggcccag ggtgagggcc ttgcggacgg cattcttggc cgggaagatc cggctcgagc
     3721 cctcgccggt cagcagcagc cggccggtct tttgaatcgc ggccagcacg gcggcgctgc
     3781 gggcaggatc gaattcctcc accagatgga tggcatccat catctcgcgg acgagagcga
     3841 aatgcgcata tttttcctgt tccagattca tcgttgggtc ctttcctcat cctgtccggt
     3901 ttatcagctt ctttgcttga tctccgcgac gaggcggaga tactccgcgc tctcctcgcg
     3961 aacctggcgg ataaaatcgt tttcatcgat cgtgccgatc gcagccgcca tctcctcatc
     4021 gttgcggaag gtcgcacggt gaaagggatt gtcgtaatcc tttttacggg aaaggtagac
     4081 ctggtcgctg acgaactgct ggattctcac cgaatcttcg agggcggcca gccactgttt
     4141 gcggttcagg cgtcggaccc ccaggacaga gcagagggaa gcgaaggcgt gtttctgttt
     4201 ggcgagggga tacttttccc acaagctggt atagcggcgg tggatggcct gccaatccct
     4261 gaggcgtccg gagacgatgt cggcgcggag ccggtctgtt tccttttcgt gcatgagctg
     4321 tccgccgaaa ttgacccagc gctcgatgcg gcgtccgctc agggcttttt gcatggcctc
     4381 aaagccggct tcaggatggg catgtatcca ggcggccagg tttttaacgc cgtagtagcg
     4441 gatcatatcg tggtaggcgc gccaggcctg ccaggtcttg aggattacca ccttgcggct
     4501 ggagcgttcc atgttttcac cgaggatttc gaggtgtgcg acctcctcct ccggcccgga
     4561 gagcaggtcg cgcccgcggg cggccagggc ctgctcgctc actccatctt ccgggcggcc
     4621 ggcgctgcgc agccaggccc gggcggtcca gatttcgagc aggcgacagg cggcaaagat
     4681 ctcctcgatg gtatcggggg cgaggcattc aaattcgata ttctgttttt tgtttttgcg
     4741 tttgtcgcgg ctgcggtact tggcgccgtt gcgggcgagg gcgtacatgt tgtacatcca
     4801 ccagaaggcg ggcatgattt cgagccggtt gagggttgca ttgttgttga gcagggagaa
     4861 ggggagaggg atatccagct cggcgggata gtccgccttg gagatgagca caaaggaggc
     4921 gaagcggcag gagtgtttga cgctggtgca gagccccggc cagaaaccgc gccccgcctg
     4981 gatctcgttg tcgttggcgc ggctgttgtg gttggagccg atggtggcgc cggcggcgat
     5041 attgctctgg cccatcacca cacccgccac caggaaggag ttgttatggt gctgttcgtg
     5101 ggcggggaag atgagagtgt tgagcacctc gcagcacgag atggtcgagt tgtcgcctag
     5161 aaaggagttg atgaggcggg cgccatactt gaggtttgaa ttgtcgccga ggacgaagcg
     5221 gaccgccttg cagccgtaaa agatgtggca gccatagccg atgatgccgt tgaccagctc
     5281 aactccctcg ccgatctggg tcggctcggc ctgcgaggag ttgatggtca ggtttttaag
     5341 tttgttggcc cccttgatgt agcagtgggc gccgattttg acatccttga gcaccaggga
     5401 gtttttaatg acgcactggt cctcgatgag gccgtaatag ccccgatggg cgtccatact
     5461 tatctgggtc atttccttga gcctggcctg gagcggccgg tcgtcgcggt acttggccca
     5521 gagccaggcg tcggcggcga tcatgccgtc gaaaggagcg acccgccggc agccggcctc
     5581 attcatcaga tccagccaga cgcgcacctc ttcgcgctcg ccctccttga cgatgccatt
     5641 gccgaatttg gcatggtcgc tggtgttcat ctcatccact ttggagagga tgcagcggtt
     5701 gccgatgatg aaatgggaga gatagccgac gttatggatg gcgacgtcat cgccgatatc
     5761 gcaggcgatg atgatgctgc cggtgatgcc ggtcggcagt ctgaggtcgt tatgttcgag
     5821 cactgatttt tgcagtcggc cgatacgcac cagtccgaag aactggttgt tggctataag
     5881 ggagggatcg aaaacgtcgg tgacgaggat gtcgtcccag ctgctcgagg tgttgttgtt
     5941 cttgaccagc gtctccacct caaccgcatg cagatggcgc cagcgctcaa gcggccagag
     6001 ggtttgcagg ttccggtatt cgtattcatc cttgccctcg ggcagccagg gtgcctggat
     6061 aaaattttta ccgatcgatc cggcgggcag aatcgaaact gtgtccataa gcgtcccctc
     6121 agctggtaga atgaaaaagc ccccggccgc ggagccgggg gctcgaaaaa gaggcttgcg
     6181 gaatcagaac cagatgcgga aaccgatgct catcggaaaa tagaggtggg tcgcgccgat
     6241 gttgggagag aaatggagtc cgatcgacgg aacgacctgg aagaagggtt cgattttttt
     6301 atcgatcatc atatttacgc cgaggggaat gcgcacgccg cccttcatgc ccttgtcatc
     6361 ctctttggtt ttatcatcat catcgaagcg tgtgctcagg aaggccccga ccccggcgaa
     6421 ccagttgaca tccgaattgc cggtaaccgt aatgcggttg ttaaagagcc agtagtcggc
     6481 ggtcaagccc agggttttct ggtcgccgag gtagtagctg agggcgagca cgagcggctg
     6541 gtcgtccatt ttcatgttga tggcgatgcc gttggtgtag agttcgccgg cgttgaggtt
     6601 gtactgcagg ccgatgccgt tggcatagct gccgctggcg aaggccagca tgaggacggc
     6661 gatcaacagt acttttttca tcttcatctg gttgctcctt gtctaggtct atgctctcgc
     6721 cggtccgtat ggagccgggc ctgctctgca ttatcgcgac acttgctgca aaatttaagt
     6781 ttatcttatg ataaaatcaa gaagtatttg ctttgcttcc tgagcgccca tgcccgccgg
     6841 gcgggtgcgc atctcttttg atccattttt cccccgacgc gcccatcatc cgaagacgcg
     6901 gattttgccg ggcaggacgt tgactgtgat cggggtggtt ccggtgattt cgccgtccgg
     6961 ggtgagcacc ttggccgggg tggagcggaa gctcatggtg ctggcggtga aatgctcgac
     7021 ctccttcatt ttcagatggg tgcccgcaaa gatccttggc agggcggaga gcaggcgcag
     7081 gcgcgacagc ttgttgagga tgacaacatc gaggaggccg tcatcgatgc tggcatccgg
     7141 ggccatgatc atgtcgccac cggtcttgcg gctgttgctg atctcgacaa agatattgtc
     7201 gcgctggaag gagcggccgt cgatttcgaa ttcgaggggg tagctcttga gattccgggt
     7261 gataataaag accccgatga catagttgag ggctccccac ttccggtagc tgtaggcctt
     7321 ttgcgccaca tcggcgacaa aaccgaagcc gaggatgttg atgaagacga agctggtgtc
     7381 gtcggagcgg cactcgccga ggtcgacggg ccgtgtcgcg cccgcggcga tcagttcgat
     7441 ggcgtcggcg tgggtgccga tgccgaggtc gcgcgaaaag gagttgcccg tgccggccgg
     7501 cagcacaccg agggggatgg gcagctccgg attgcccttc atcatgccgt tgatgacctc
     7561 gaagagggtg ccgtcaccgc cgagggcgat gatgccatcc caggctccgt tgacctcctt
     7621 agccgccacc tcgacgaggt ggccggggcg atcggaaaag atattttcca ccgtatgacc
     7681 tcgctgccgg agcatctcga gggatttttc ggcgacggcc agcccgcgtc tgcggccggc
     7741 caggggattg gagagcatga gcagcttcat gggccatcca ttcttgtggg gaaccgtttg
     7801 atctataata atgatatttg ggctcaattg caaggggttc cgggccagat tttcccaaga
     7861 aatctggttt ccaccttgcg ccgctttttt aaaatttaat gcgggtggga ctcttatttg
     7921 aggggcatca aggttgtgtg cctgctttta tttgccttga tttttgcgcc ctgattggct
     7981 aatttccgtg ggagagcccc gacatggaga tgaccagagt ggacaggagg cgcgcgcagg
     8041 cggccgcgcc ggagatcgat catcagcagg tgagctggag ggatctgcgc gaatcggcca
     8101 accgcgcctt ttcaagagcg gctggagcgc ccctcattcc cggcaaccgc atccgcctgc
     8161 tcaaggatgc agccgaaaac tatccggcct ggctggaggc gatcgccgcg gcccggaagc
     8221 atatccatct cgagagttat atcatttatg gggatgcgac cggccgcaga ttcgccgatg
     8281 ccctgctgca aaaggctgct gaaggagtat gcgtccgggt catctatgac tggctggggg
     8341 gattcggcaa aaccccgcac aaattctggg agcggctgcg taccggcggc attcaggtgc
     8401 gctgcttcaa ccgtccgcgc cttgatcgtc ccctcggctg gctctcgcgc gaccaccgca
     8461 agatgctggc ggtggacggc caggtcggct acgtctccgg gctctgcatc ggcatggcct
     8521 gggaggggct ccccgccaaa aaaatcgagc cctggcgcga taccggggtg gaggtgcatg
     8581 ggcccgcggt cgctgacatt gaaaaggcct tcgcccaggt ctgggcgatg accggggagg
     8641 aactccccga cggggaggag ggcgccggcg ggatcatcgc cccggccggg acggccagcc
     8701 tccgcgtcgt cgccaatatt ccggccaccg ccggcctttt ccgtctcgat cagctcgtcg
     8761 ctgctctcgc ccgtgagcgg ctctggctga ccgacgccta ctacgccggc aacaccccct
     8821 acgtgcaggc cctgcgctcg gcggccgtgg acggggtcga tgtgcgcctg ctggtgccca
     8881 atgccaccga tatccccgtg gtcaaagccc tctcccgcgc cggctaccgc acattgctcg
     8941 aatcgggggt gcgcgtcttt gagtggaacg gcaccatgct ccatgccaag accgccctcg
     9001 ccgaccggca ctgggcgcgg gtaggttcga gcaatctcaa catctcgagc tggctcggca
     9061 actgcgaact cgatgtcgtg gtggaggatg agcatttcgc tgccgggatg gaggcgatgt
     9121 atctcgcgga tctggacaat gcgaccgagg tggtgctgag cagaaaacag cgggtgctgg
     9181 cgccgggaga gcctccccat cccgcccgca ccatcaccag cggatcaggc agcgccgggc
     9241 gtgcagccgc cggagcggtg cgcatcggac acgccgtggg cgcggccttc accggccgcc
     9301 gcctcctcgg ccagatcgag gcccggctga tgaccctgat cggagtgctc ttcctcctcc
     9361 tggccgc
//
LOCUS       DAOPWU010000117         9341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7410_length_9341_cov_4.522830, whole genome shotgun sequence.
ACCESSION   DAOPWU010000117 DAOPWU010000000
VERSION     DAOPWU010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7410_length_9341_cov_4.522830"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKM23_07600"
     CDS             <1..381
                     /locus_tag="PKM23_07600"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNY91376.1"
                     /translation="WVHSQHMAFPLPSIVRLLRYIRIPHQHVELSRRNIIKRDNFRCQ
                     YCGTTRGPFTVDHVIPRVRGGSDSWDNLVCACVRCNNRKGDRSPEQANLKLIKNPRRP
                     SHLFFIQHHMGISEDCWKPYIFLN"
     gene            526..1272
                     /locus_tag="PKM23_07605"
     CDS             526..1272
                     /locus_tag="PKM23_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNY91377.1"
                     /translation="MSYKVKLESFEGPLDLLLFLIKKEEVDIYDIPIAKITQQYLQYL
                     EIIQLLDLEAASDFILMAATLMRIKAQMLLPKPDLGEEQQEITDPRQELVQRLLEYKR
                     FKDVAEDLGSKEETTLKQYGRGNFKVEEETIGEDFQPGSEVGLFDLVAAFKQIMDQSK
                     KVTVHRVVEITTTLEECMANVLDALDKKSELTFRELFVPESDKIVWVVTFIAILELIK
                     RGAIRAVQDEPFAEIMIRRINGAEQTQVDS"
     gene            1241..1987
                     /gene="scpB"
                     /locus_tag="PKM23_07610"
     CDS             1241..1987
                     /gene="scpB"
                     /locus_tag="PKM23_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNY91378.1"
                     /translation="MERNKLKSILEAMIFASDTPLSMNQIRVILEGVEKADIDGALAE
                     LAEDLKERAFFLKKAGGGYQFATRPEYFRWIKQMFAGRERNRLTRAALETLSIIAFKQ
                     PISRVEVAAIRGVNSDGVMQTLLERKLISITGRDEGQGRALLFSTTKEFLIYFGIDDI
                     SDLPKPKEIEELLAMGEGQKAIREIPEEEILAREVEEEADAGAIDGEPAPPSAAVTSP
                     PAEAASGDVAEPPLDSAPAAEGALLADDAH"
     gene            1974..2699
                     /locus_tag="PKM23_07615"
     CDS             1974..2699
                     /locus_tag="PKM23_07615"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNY91379.1"
                     /translation="MMRINRFLASCGLGSRRAVETLVRDRRIAVNGEIVADLATRIDE
                     AADRVTLDGQPVGRKSESIYVLLNKPKGYITTASDEKGRRTVLELVALPQRIFPVGRL
                     DYNTSGLLLLTNDGELAHRLTHPRFKVPKLYHAVLESAFNPDHFAPLTQGVTLEDGIT
                     QPCKARYYGDSRSEVEIELREGRQQQVRRMFAALGYRVRHLNRVRFGPLRLERLPRGE
                     WRYLMDEEILALRTAVGLVQAGE"
     gene            2703..3392
                     /gene="cmk"
                     /locus_tag="PKM23_07620"
     CDS             2703..3392
                     /gene="cmk"
                     /locus_tag="PKM23_07620"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNY91380.1"
                     /translation="MEIRKKRLIITIDGPAGSGKSTTARRVAEQLGYLYLDSGALYRA
                     VTLAALRRGADFGNPDELASIARECEIGLVFASTGLKVYLQGEEVSEEIRLPEVAGAI
                     GPVAANPGVRQALLSQQRRLGENGGIVAEGRDMGSVVFPEAEVKVFLVASIEERARRR
                     RMELAAKGIEVDLEELIRTIAKRDRDDSGREISPLCKPEDALELDTTSLTIAEQVEWI
                     VRAARQRGAGA"
     gene            3389..4264
                     /gene="ispH"
                     /locus_tag="PKM23_07625"
     CDS             3389..4264
                     /gene="ispH"
                     /locus_tag="PKM23_07625"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNY91381.1"
                     /translation="MKVIIDPQAGFCPGVRRAVAQVETRLQRGESLTALGALIHNQRE
                     IDRLAGLGLETVSQEVADRPEELAALKGRELFIRTHGVGSALQNQLHESGFDIIDGTC
                     PTVKRVQKLAAEHHRRGEQIVIIGKKGHAEVIGILGHCDNMGIVVADEEDLMIIDPER
                     KTFVIAQTTIGGEQFRRLCRLIAGRAPGATFMDTTCPFITRRYDQIAGFAASVSLLIF
                     VGGRESSNSKVLFEICRQRNPRSHRIESPAELDYTWIRPEDTIGITGGASTPQWQFEE
                     IRDLLGQAATQLESK"
     gene            4316..6334
                     /gene="rpsA"
                     /locus_tag="PKM23_07630"
     CDS             4316..6334
                     /gene="rpsA"
                     /locus_tag="PKM23_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNY91382.1"
                     /translation="MDELTNTNLSAEEAGQIYPLPEEVKPETAASDAEPETAAEPVQT
                     PAAEAEPVQAFAAEPVQAATQPEPTLESLDLLENEYTPQERESLEKLYEETLADFNSG
                     VVVTGKVLAINDKEVAVDIGFKSEGTIPIDEFADPKEINVGDNIEVYIDTIEDSDGML
                     ILSKKKADFMRVWEMVNNSYEKGEIIQGRCTRRIKGGIVVDLSGVDAFLPGSQIDVRP
                     IRDFDALIGQTMDFKIVKINNLRKNIVVSHRVLVEEAMKGQREKILEDLQKGQILEGA
                     VKNITDFGVFIDLGGVDGLLHINDLSWGRVAHPSEVVALDEKIKVVVLDFNEDKDRIS
                     LGLKQLQPHPWEDVENKYPVGSVIRGKVVSISDYGAFVELEKGVEGLIHISEMSWTQH
                     IKHPSKVLSVGEMVEAKVLNIQKEERKISLGLKQLEPDPWQTLSERYPVGSRHKGKVR
                     NLTNFGAFVELEDGIDGLIHISDLSWTKKIRHPGEVVKKGDNIDVVVLNVDRDNRRIS
                     LGYKQTQDNPWDFFEDKYHPGALVEGKVVRLIEKGLIVELPDSVDGFVPMSQLAKDNL
                     SKPADAYQIDDALTLAVIEFNKENKKIVLSEKNAIRMENREAKAAAAAEKSAAETAPA
                     PEAEEEPAAKVVDEVDSETPAGESAPEEGVEESAEEKPKRARKKKSEE"
     gene            6504..7316
                     /locus_tag="PKM23_07635"
     CDS             6504..7316
                     /locus_tag="PKM23_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNY91383.1"
                     /translation="MHRMILLFLALLLTGAAPLLHSQEGLAELERAWQEDRFDDLEKL
                     LPAAEKAYPRNPTVAFLRALVLADADEAFASYKRVFESEEMSRYADTALFKMAQYQYA
                     RERFTAARRYFQMMQKRFPQTRWLDDSLYLTGQCYLAEGKSDSARLVWNSLIKRFPRS
                     PYCDMAVADLESSVWDQPASAPRQSAAPVSGGKPFYSIQVGAFSKQENAHTILAGLEK
                     VGYEGMIVEKKVGTRLFYAVWIGSFPDRESAEDYARRFIVKVTKEYSIVKSE"
     gene            7325..8206
                     /locus_tag="PKM23_07640"
     CDS             7325..8206
                     /locus_tag="PKM23_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNY91384.1"
                     /translation="MKTEILTAVKEGAILFDGAIGTQLQARGLAIGSSPESWNRLHPD
                     LVASVHRTYLQAGAQVLTTNTFGGSPHKLFTDGVEGDPEEINRLAAAIAREAAGDQAW
                     VAGSIGPTGTMLMMGEVGEDEILEGFLCQARGLATGGADLIVIETMSDLTEALLALKA
                     ARTVCDLPVFVSLTFSPGQRGYRTLMGVDVSQAAAALAKAGAAVMGCNCGTGIDDAIH
                     IVREIAVSWKGPVLAEPNAGLPQLIDGKTSYQETPEKMAARLPELIGAGAVFVGGCCG
                     TTPDHIRAFHHQLKGEV"
     gene            8208..8960
                     /locus_tag="PKM23_07645"
     CDS             8208..8960
                     /locus_tag="PKM23_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNY91385.1"
                     /translation="MLKRILLIIDASPQMEIAADYALAIAARHGSQLLLFPLAEKSSA
                     PAGAASGELLLGPTVQRAEAAGLSYQVLSTSALPFSRSLEWSRCSDIAVLPYLAPKSA
                     ARMEKRVVRLLTDGGCPVLLPSAAPLESKVAAVAWDGSSAMARALKLHLQLFRRSRLR
                     YLLVHMGDHPDKALSILEQGCALVEAQGAAAETLALSGAPVPRILELSASIRLNHLIV
                     APHSRRLFSKQRLGPTSGKLLRSRVNSLFLAT"
     gene            9089..>9341
                     /locus_tag="PKM23_07650"
     CDS             9089..>9341
                     /locus_tag="PKM23_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91386.1"
                     /translation="MKIQIAHPITSYFDATSQLLRAQGEDFHLTGATSLPEVFDSSAQ
                     KLEDVYVIDSILLRDEPSETLDQIGAMSRHVPTIIMIEES"
BASE COUNT         2000 a         2899 c         2670 g         1772 t
ORIGIN      
        1 tgggtccatt cacaacatat ggccttcccc ctgccgagta tcgtgcgttt gctgcgctat
       61 atccgcatcc ctcatcaaca cgtcgaactg tcgcgccgca acattatcaa acgggacaat
      121 ttccgctgcc agtactgcgg gaccacaaga gggcctttca ccgtcgacca cgtcatcccc
      181 agggtgcgcg gcggctccga cagctgggat aatctggtct gcgcctgtgt gcgctgcaat
      241 aaccgcaagg gagaccgctc gcccgaacag gccaacctga agcttataaa aaacccgcgg
      301 cggccaagcc atcttttttt tattcaacac catatgggta tcagcgagga ctgctggaaa
      361 ccctatatct ttttaaattg accgtggcta tccagataca gcgcatctct tcttctgctg
      421 ccaaggccgt gattccgacg gccttcgtca tgtcaagcgg aaccggcgct tccggcaggc
      481 cttccacttg aaaacgcttc acacggtgag gtctcctggc tgcttatgag ttacaaggtc
      541 aaactggaaa gttttgaagg gccgctggat ctgctcctct tcctgatcaa aaaggaggag
      601 gtcgacattt atgatatccc gatcgccaag atcacccagc agtacctgca gtatcttgag
      661 atcatccagc tccttgacct ggaggcggcg agcgatttca tcctcatggc cgccaccctg
      721 atgcgcatca aggcccagat gcttctgccc aaacccgacc tcggcgaaga gcagcaggaa
      781 atcactgatc cgcgccagga gctggtccag cgcctcctcg agtacaagcg cttcaaggat
      841 gtcgctgagg acctgggcag caaggaggag accaccctca agcagtacgg ccgcggcaac
      901 ttcaaggtcg aggaagagac catcggcgag gatttccagc ccggctcgga ggtgggcctc
      961 tttgacctgg tcgccgcttt caagcagatc atggaccagt ccaaaaaagt gacggtccac
     1021 cgcgtggtcg agatcaccac cacgctcgag gagtgcatgg ccaacgtcct cgatgccctt
     1081 gacaaaaaaa gtgagctgac ctttcgcgaa ctattcgtcc ccgaaagcga taaaatagtc
     1141 tgggtggtga ccttcatcgc catcctggaa ttgatcaagc ggggcgcgat tcgtgcggtg
     1201 caggatgagc cctttgcgga aataatgatc agaaggatta atggagcgga acaaactcaa
     1261 gtcgattctt gaagccatga tcttcgcctc cgatacaccc ctttccatga accagatcag
     1321 ggtgatcctc gagggggtgg aaaaggcgga tatcgatggc gccctggcgg agttggccga
     1381 ggatctcaag gagcgggcct tttttctcaa aaaggcgggg ggaggttatc agttcgccac
     1441 ccggcccgag tattttcgat ggatcaagca gatgtttgct ggacgggagc gcaaccgcct
     1501 cacccgagcc gcgctcgaaa ccctctccat catcgccttc aagcaaccga tcagcagggt
     1561 ggaggtcgcc gccattcgcg gcgtcaactc ggatggcgtt atgcagaccc tgctcgagcg
     1621 caagctgatc tccatcaccg gccgcgatga gggccagggc cgcgccctgc tcttcagcac
     1681 caccaaggaa tttctcatct acttcggcat tgacgatatc tccgatctgc ccaaaccgaa
     1741 ggagattgag gaactgctcg ccatgggcga aggccaaaag gccatccggg aaattcccga
     1801 ggaggagatt ctcgcccggg aggttgagga agaggccgat gccggcgcca tagacggcga
     1861 gcccgcgccg ccctcagcag ccgtaacctc ccctccggcc gaggccgcct ccggggatgt
     1921 ggcggaacca ccactcgact ccgcgcctgc cgccgaggga gccctgctgg ccgatgatgc
     1981 gcattaaccg ttttctggca tcctgcgggc tcggctcacg ccgggcggtc gagaccctcg
     2041 tccgcgaccg gcgcatcgct gtcaacggcg agatcgtcgc cgacctcgca acccgcatcg
     2101 acgaagccgc cgatcgggtc acactggatg gccagcccgt cgggcgcaag tccgaatcga
     2161 tttatgttct tttaaataag cccaagggct acatcaccac cgccagcgac gaaaaaggcc
     2221 gccgcactgt cctcgaattg gtcgccctgc cccagcgtat tttcccggtc ggccgtctcg
     2281 attataatac cagcgggctg ctcctgctca ccaatgatgg cgagctggcc cacaggctga
     2341 cccatccccg cttcaaggtg cccaaactct accacgccgt gctcgagagc gccttcaatc
     2401 cggaccattt cgccccgctg acccagggtg tcacccttga ggatggcatc acccagccct
     2461 gcaaggcccg ttattacggc gattcccgca gtgaggtcga aattgagctg cgcgagggcc
     2521 gacagcagca ggtgcgccgc atgttcgcag ccctcggcta ccgggtcagg catctcaacc
     2581 gcgtccggtt cggaccgctg cggcttgaac ggctgccgcg cggtgaatgg cgctacctca
     2641 tggatgagga gatcctcgcc ctgcgcaccg cggtcggtct ggtgcaggcc ggggagtgaa
     2701 ccatggaaat acggaagaaa cggctgatca tcaccatcga cggaccggcg ggatcgggaa
     2761 aaagcacaac cgcgcgccgc gtggccgaac agctgggtta cctctatctc gattccggag
     2821 ccctttaccg cgccgtcact ctggccgccc tgcgccgggg agccgatttt ggcaatcccg
     2881 atgaactggc ttcgatcgcc agggagtgcg agatcggact ggtcttcgcc agcacaggac
     2941 tcaaggttta tttgcagggt gaggaagtca gcgaggagat ccggctgccc gaggtcgccg
     3001 gggccatcgg cccggttgcg gccaatcccg gagtacgcca ggccctgctg agccagcagc
     3061 gccgcctggg agaaaacggc ggtatcgtcg cggaggggcg cgatatgggc agcgtggttt
     3121 ttccggaggc ggaggtgaag gtctttctcg tcgcctccat tgaggagagg gcgcgccggc
     3181 ggcggatgga actggctgcg aagggaattg aggtcgacct ggaggagctc atccgtacaa
     3241 tcgccaaaag agaccgggac gacagcgggc gtgaaatcag ccccctttgc aagccggagg
     3301 acgctctcga gctggacacc acctcgctga ctatcgccga gcaggttgag tggatcgtcc
     3361 gtgccgcccg gcaaagaggc gccggcgcat gaaggtcatc atcgatcccc aagccgggtt
     3421 ttgtcccggc gtacgccgtg ccgttgccca ggtcgagaca cgcttgcagc gcggggaatc
     3481 cctgaccgct ctcggtgccc tcattcacaa ccagcgggaa atcgaccgcc tggccgggtt
     3541 gggcctggag actgtaagcc aggaggtggc tgaccgcccg gaggagctgg ctgccctcaa
     3601 aggacgggag cttttcatcc gcacccatgg agtcggcagc gctcttcaaa accaactgca
     3661 cgagtcgggc tttgacatca tcgatggcac ctgcccgacg gtgaaaaggg tgcaaaagct
     3721 ggccgccgaa caccaccggc ggggcgaaca gattgtcatc ataggcaaaa agggccacgc
     3781 cgaggtaatc ggcatcctcg gtcactgtga taatatgggc atcgttgtcg ctgacgaaga
     3841 ggatctcatg attatcgatc ctgaacgaaa gactttcgtc atcgcccaga ccaccatcgg
     3901 cggcgagcag ttcaggcgcc tgtgccgcct catcgccggg cgtgcgccgg gggccacctt
     3961 tatggatacc acctgcccct tcatcacccg gcgctacgat cagattgccg gtttcgccgc
     4021 cagtgtatct ctgctcattt tcgtcggcgg acgcgagagc tccaactcaa aggttctatt
     4081 cgaaatctgc cggcagagga acccgcgcag ccaccggatc gaatcccccg ccgagctgga
     4141 ttacacctgg atccggccgg aggatacgat cgggattacc gggggggcgt caaccccgca
     4201 gtggcagttc gaggagatcc gcgatcttct cggccaggct gctacacagc tcgagagcaa
     4261 ataaaaaact tgacccagtt gggcaagtat tcatacaaaa ggagaaagaa aaataatgga
     4321 cgaactaacc aacaccaacc tgtccgctga agaggctgga caaatctatc ccctgccgga
     4381 agaggtcaaa ccggaaacgg ctgcctccga tgctgaaccc gaaaccgctg cggagcccgt
     4441 acagacccct gccgccgagg ctgaaccggt gcaggctttt gctgccgagc ccgtccaggc
     4501 cgccacacag cccgagccca ctctggaatc ccttgacctg ctcgagaacg agtacacacc
     4561 gcaggaacgc gagagcctcg aaaagctcta cgaggagacc ctcgccgact ttaattccgg
     4621 cgtcgtggtc accggcaagg tgctcgccat caacgacaag gaagtggccg ttgatatcgg
     4681 cttcaagtcg gagggaacca tccccatcga cgaattcgcc gatcccaagg agatcaatgt
     4741 tggggacaat atagaggtct acatcgacac catcgaagac tccgatggca tgctcatcct
     4801 gtcgaagaag aaggccgact ttatgcgcgt ctgggagatg gtcaacaact cttatgaaaa
     4861 gggtgagatc atccagggcc gctgcacccg ccgcatcaag ggcggtattg tcgtcgatct
     4921 ctccggcgtc gatgccttcc tgccgggctc gcagatcgat gtccgcccga tccgtgattt
     4981 cgacgccctc atcggccaga ccatggattt caagatcgtc aagatcaata acctgcgcaa
     5041 gaacatcgtc gtctcccacc gcgttctggt cgaagaggcg atgaagggac agcgcgaaaa
     5101 gattctcgaa gatctgcaga aaggccagat cctcgagggg gccgtcaaga atatcaccga
     5161 tttcggcgtc tttatcgacc tcggcggcgt cgacggcttg ctccatatca acgacctctc
     5221 ctggggccgc gtggcccatc cctccgaggt ggtcgcactg gacgagaaga tcaaggtcgt
     5281 cgtcctcgat ttcaacgagg acaaggaccg catatctctt ggactcaagc agctccagcc
     5341 ccatccctgg gaggatgtcg agaacaaata cccggtcggt tctgtgatcc gcggcaaggt
     5401 cgtcagcatc tccgattacg gcgctttcgt cgagctcgaa aagggcgtcg aaggcctcat
     5461 ccatatctcc gagatgagct ggacccagca catcaagcat ccctccaagg tcctctccgt
     5521 gggcgaaatg gtggaagcca aggtgctcaa tatccagaaa gaggaacgca agatctccct
     5581 cggtctcaaa caactcgaac cggatccctg gcagaccctg tcggaacgct acccggtcgg
     5641 ctcgcgtcat aagggcaagg tccgcaacct caccaacttc ggcgccttcg tcgaactgga
     5701 agacggcatc gatggtctta tccatatctc cgacctctcc tggaccaaga agatccgcca
     5761 tcctggcgaa gtcgtgaaaa agggtgacaa tatcgatgtg gttgtcctca atgtcgaccg
     5821 cgacaaccgc cgcatttcgc tgggctacaa gcagacccag gataatccct gggacttttt
     5881 cgaggacaag taccatcccg gcgccctcgt cgagggcaag gttgtgcgcc tgatcgaaaa
     5941 gggactcatc gtcgagctgc ccgattcagt ggacggcttt gtgcccatgt cccagctcgc
     6001 caaggacaac ctcagcaagc cggctgatgc ctatcagatc gatgacgccc tcaccctcgc
     6061 cgtgattgaa ttcaacaagg agaacaaaaa aatcgtcctc tccgagaaga atgcgatccg
     6121 gatggagaac cgggaagcca aggccgccgc tgccgcggaa aagtccgcgg ccgaaaccgc
     6181 tccggccccg gaagcggaag aagagcccgc tgctaaagtc gtcgatgagg tggatagcga
     6241 gactccagcc ggggaatccg ctcctgaaga gggagtggaa gaatcggcgg aagagaaacc
     6301 caagcgcgcc cgcaagaaga agagcgaaga gtagaacttt cctgcaggcc ctgcttttgt
     6361 cagactggag ttggatccga ccggatccaa ctccttttta tttcaggcat cgctgctgta
     6421 aaaagctgtt tgaaatttgg ctgaataata atacattgca gcagcggaat gacccgcagc
     6481 tgcagatgat gagatccagg cctatgcata gaatgatctt gctgtttctt gcccttctct
     6541 tgaccggcgc cgcgccgctg ctccacagcc aggaaggcct ggctgagctg gagcgcgcct
     6601 ggcaggagga ccgtttcgat gatctcgaaa agctcctgcc cgcagcggag aaggcttatc
     6661 cccggaatcc caccgtcgcc tttttgaggg ctctggtcct tgctgacgcc gatgaagcct
     6721 tcgccagcta taaacgggtc ttcgaaagcg aagagatgag ccgctacgcc gataccgccc
     6781 tcttcaagat ggcccaatat cagtatgcac gcgaacgctt cacagccgcg cgcaggtatt
     6841 tccagatgat gcagaaacgg tttccacaga cccgctggct ggatgacagc ctctatctga
     6901 ccgggcagtg ttatctcgct gagggcaaat ccgactcggc gcgcctggtc tggaactcgc
     6961 tcatcaaacg cttcccccgc tctccctatt gcgatatggc cgtcgccgac cttgaatcgt
     7021 cggtctggga tcagcccgcc tcagcccccc gccagtcagc cgcaccggtc tccggaggca
     7081 agcccttcta ttctatccag gtcggggctt tcagcaagca ggagaacgct cacaccatcc
     7141 tcgccgggct ggaaaaggtc ggttatgagg ggatgatcgt cgagaagaag gtcggtaccc
     7201 gcctctttta tgcggtctgg atcggcagct ttcccgaccg cgagtcggcg gaggattatg
     7261 cccgccgctt catcgtcaag gtcacgaagg aatacagcat cgttaaaagc gaatgagact
     7321 gaagatgaaa acagagatcc tcaccgctgt aaaggagggg gccatcctct ttgacggcgc
     7381 catcggcacc cagctccagg cccgcggcct cgctatcgga tcctcgccgg agagctggaa
     7441 ccggctccat cctgatctgg tggcctctgt gcaccgcacc taccttcagg caggcgctca
     7501 ggttctgacc accaacacct ttggcggctc accgcacaag ctttttacgg acggggtgga
     7561 gggcgatccc gaggaaataa accgtctcgc cgccgctatc gctcgcgaag ccgccggcga
     7621 tcaggcctgg gtcgccggct ccatcggtcc caccggcacc atgctcatga tgggcgaggt
     7681 cggcgaggat gagatcttgg agggattcct gtgccaggcc aggggactcg ccaccggcgg
     7741 cgcggacctc attgttatcg aaaccatgtc cgatctcacc gaagccctgc tcgcgctcaa
     7801 ggctgctcgc acggtttgcg atctgcctgt tttcgtctcc ctgaccttct cgccggggca
     7861 gcgcggctac cgcaccctca tgggtgtcga tgtctcccag gctgcggcgg cactcgccaa
     7921 ggcgggcgct gcggtgatgg gctgcaactg cggtaccggc attgatgatg ccatccatat
     7981 cgtccgggag atcgccgtca gctggaaggg ccctgttctc gcagagccga atgccggcct
     8041 gcctcagctt atcgacggca aaaccagcta tcaggagacc ccggaaaaaa tggcggccag
     8101 actgccggaa ctgatcggag caggagccgt ctttgtcggg gggtgctgcg gaaccacccc
     8161 cgatcacatc cgggccttcc atcaccagct caagggggaa gtgtgacatg ctgaaacgaa
     8221 tcctgcttat catcgatgcc tcgcctcaaa tggagatcgc agccgattac gctcttgcca
     8281 tcgctgccag gcacggcagt caactcctgc tctttcctct ggcggagaaa tccagcgccc
     8341 ccgccggggc ggcctcggga gaacttttac tcggtccgac tgtgcagcgc gccgaagcag
     8401 ctggcctctc ctatcaggtg ctctcgacct cggccttgcc cttctcgcgc agcctggagt
     8461 ggtccagatg cagcgatatc gcagtgctgc cctatcttgc tcccaaatcg gcagcgcgca
     8521 tggagaagcg agtggtgagg ctgctgaccg acggcggctg ccccgtcctg ctgccttcgg
     8581 ctgcacccct ggaatcgaag gtggccgcgg tggcctggga tggctccagc gccatggcgc
     8641 gcgccctcaa actccatctc cagctcttcc gccgttcccg cctgcgctac ctgctcgtcc
     8701 atatgggtga tcatccggac aaggccctct ccatccttga acagggctgc gctctggtcg
     8761 aggcccaggg agcagcagcc gaaaccctgg ccctttccgg agcaccggtg ccgcgcatcc
     8821 tcgaactctc cgcctccatc cggctcaacc atctcatcgt cgcaccgcac agccggaggc
     8881 ttttcagcaa gcagcgcctc ggcccgacca gcggaaaact cttgcgcagc cgggtcaact
     8941 ccctttttct tgccacctga gcggatgagg ctgaattcat tgaagaattt attatttaaa
     9001 agcttgacta attttttaag ttttgttaat tttacgcata aatattgccg accacgtgta
     9061 gtgacgggaa aacgggagaa acggtggcat gaaaatccag atagctcacc ccatcacctc
     9121 ttatttcgat gcgaccagcc agctgctcag agcccaggga gaagattttc atctcacagg
     9181 cgccacctct ctgccagagg tttttgattc gagcgcgcaa aagctcgaag atgtctatgt
     9241 catcgacagc attctgctgc gcgacgagcc ctccgagacc ctggaccaga tcggcgccat
     9301 gagccgccat gttccgacca tcatcatgat cgaagagagt g
//
LOCUS       DAOPWU010000118         9320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7432_length_9320_cov_4.423637, whole genome shotgun sequence.
ACCESSION   DAOPWU010000118 DAOPWU010000000
VERSION     DAOPWU010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7432_length_9320_cov_4.423637"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PKM23_07655"
     CDS             <1..678
                     /locus_tag="PKM23_07655"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNY91387.1"
                     /translation="AAAMGIENLLCLTGDHQKFGDHPEARGVFDLDSIQLIAAVQQMN
                     AGSLLSGQFLKKAPAFFIGAAANPFAEPLEMRLIRLEKKVRSGAAFIQTQPVFDLDQF
                     SRWLELVRGRGLHEKSAILAGVLPVRSAKALEYMAREVPGMRINPRYLERMRTAAEPQ
                     EEGVAIAVEIIGALRAMAGVAGIHLMPVMWESITPRIIQEAGLMPPEAPAPACAPCAG
                     EENPINP"
     gene            694..2076
                     /gene="gatD"
                     /locus_tag="PKM23_07660"
     CDS             694..2076
                     /gene="gatD"
                     /locus_tag="PKM23_07660"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HNY91388.1"
                     /translation="MSTEYKGYRGEALAALENFQAPVWSDVRVVTGKGSYSGIVLPRS
                     ETADPRHIVLKLRSGYNIGIAAASVTAIEVLGRKEAHYKIPEKEFPFDPKKPRVKLLG
                     TGGTIASRLDYRTGAVIPAFSPGELYGSVPELADLCNLETEKLYGVFSENMGPEQWIG
                     TAQAIGREIEKGVQGIVIGHGTDTMHHTAAILSFMVQNSPVPIVMVGSQRSSDRPSSD
                     AALNLMHSVKTAAESDIAEVMVCMFGPTSDVYGLLHRGTRVRKMHSSYRSTFRTIGDI
                     PLAMVSRDRITPLRQDYKRRRHDREVTINTAFEEKVSIVYYYPNMKPDIIDALIDNGY
                     RGIVIAGTGLGHVNKPLYPALKRAREKNIAVYMTVQTLWGYVQMYVYDTGRDMMELGV
                     TPMANMLPEVAYVKLGWALGQTSDLKRVREIMYTPVNGEITEREPSNGYLIFQGGIPE
                     VEEFISQIKK"
     gene            complement(2154..2375)
                     /locus_tag="PKM23_07665"
     CDS             complement(2154..2375)
                     /locus_tag="PKM23_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91389.1"
                     /translation="MTEFCRIVTLKNEIEAQVLAEALREREIPHVVRTFHDSAYDGIF
                     QMQKGWGVLMAPESFRAEIETILNELNAG"
     gene            complement(2338..3735)
                     /locus_tag="PKM23_07670"
     CDS             complement(2338..3735)
                     /locus_tag="PKM23_07670"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY91390.1"
                     /translation="MKILLVEDEKISRITLTDTLTKQGYQVTPCETGRAGREALEKYS
                     FEVVITDLRLPDLNGLELVKLAKMRENSCTVIVITGFATVETAVAALKLGAYDYLTKP
                     FSPDKLLSMLANIQQLHSVIRENIQLKKQITHYQGREIIGSAAVMQRLVKTLRSVAAT
                     DHTVLIEGESGTGKELVAHFLHQNSERGKGPFVAVNCAVLPESLLESELFGHEKGAFT
                     GAHKRHLGYIERAHDGTLFIDDIDDLPMRLQVKLLRVLQEHEIQPLGGQETVPVNFRV
                     ICATKANLLQMVRENTFREDLYYRINIIALTLPPLRERREDIPLLIDHFIAKHHRGAR
                     PVEITPEQLQKMMAYDWPGNVRELENTVQRLLALPQEMDYMPAGSMGDRGRAEKAAPE
                     QTEGPAGYGSYEEYILSREREIITWAMRECSQNISAAARQLKIPRSTLQSKLENLNRK
                     EGRNDGILPDRNTEE"
     gene            complement(3732..5192)
                     /locus_tag="PKM23_07675"
     CDS             complement(3732..5192)
                     /locus_tag="PKM23_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91391.1"
                     /translation="MMRSLKARIVLFLGILMIVLTMMISIILLYQWRSLILENQRQNA
                     LAVARTFAASILDGLIYQESGLLPNEGYLENQIHNFLGKNNQVRWLVMYDRDGHVAVR
                     SSFESSDPGDAGEAEALPPAAGRTGTRIYRSRSFGWILETALPLQIHSKSWGELHMGF
                     DLEPARARLQRLFFLIFGLAVLFVVSITAFIWFFINRVTQSLGRLVTEMKQFDLDNIT
                     PTQMKAGEDEIGVLVSNFEKMKQRLQQSRQQLLNAQRQIYHAEKLASIGRLASGVAHE
                     INNPLHGMKSCLYAIAQEPRNLPEVKRYLGLADEALDHIALIVQKLLGFSRQHAKQTV
                     AMELNNEIEKVLSLLTYRLSKNQIIIEKKFQSDLPLLHADPHLIQEVIMNLLLNSMDS
                     MEEEEGGTIRITTARNGEGHIHLEIADTGCGIDSRDLDKIFDPFFTTKDEGKGTGLGL
                     SVSLGIIESHGGTITCQSRPYRGTTFHITLPIQGAK"
     gene            complement(5189..6118)
                     /locus_tag="PKM23_07680"
     CDS             complement(5189..6118)
                     /locus_tag="PKM23_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNY91392.1"
                     /translation="MKSRIALILLILIFFLALTLLVYRNFMDITTAPDTVFPPAAPLA
                     AAGGRDTVHVGVISRFSPNLIYEGYQPIMDYLSRETGIIFALRLSSSYEETIAQLDQG
                     QIQSAFLGTYLYLKARRDHPIRCILKPLNSQLQPYFHSMVITRDDSPIRSISDLAGKR
                     LALPSALSFSGNWLLGYELQRHGLKVSDLDSVRYFGFHHTVVYQVLLGRFDAGVVKDR
                     VAAEFTGKGIRVIAVSREIPGSPIIVRRDLDPLITEAMVKALLRIDARRPDHRALVRD
                     WDPEFAWGFVPAEESDYDQVDAIFAALEKEPRR"
     gene            6372..6998
                     /locus_tag="PKM23_07685"
     CDS             6372..6998
                     /locus_tag="PKM23_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009422572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91393.1"
                     /translation="MPEIKPRFEFRAFAQSFGIVEEKMRAISPVEQIRESLEIYIMSA
                     GNNENNTKIRNDLMDIKVLVQVAKGLEQWNPRMKGQFPMAVEMIKNEVFPAFGVAMPE
                     FKRESYTLAQYINEIIKPHPDLAAVNVFKRRMGFTINGCIAELAEVYVNGARIMTANL
                     ESTDVEAILAAKKMIALEDFENVNYLLAIKRVIGMEPLSDTSMYKATF"
     gene            7074..7541
                     /locus_tag="PKM23_07690"
     CDS             7074..7541
                     /locus_tag="PKM23_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNY91394.1"
                     /translation="MANEVERKFLVKGEFAPFAHKKTRIIQGYLSSVPERTVRVRVKG
                     DKGFLTIKGIGNASGASRYEWEKEIPVSEAEELLKICEPGVIDKCRYQITAGKHTFEV
                     DEFYGENQGLVVAEVELSAEEESFIKPAWLGEEVTGDVRYYNSMLMKTPYTRW"
     gene            7612..9207
                     /locus_tag="PKM23_07695"
     CDS             7612..9207
                     /locus_tag="PKM23_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNY91395.1"
                     /translation="MTTFDPLDMHNYRIEQLPKRAKGRFEKGLAATGPFLALAAFILF
                     GWLVKLPFLQNIDAAMLTSAQAKAAFAKIGAEAFIRSNEMMLAIFAASLILWMTEAIP
                     NYLTSLIVIIALVLTSVLPEKTAYAQLGHEVMWLNIMSFVLASMLVATGVAKRFALWF
                     ILRFGKNANRIFLSFIFINLLLSAFISATTAKAAILLPIFMVIAAIYGARGGEEKNNF
                     GRSIVIQNLFEINVGASAFMTGSGANLLAVSLIAGAIGTQVFFSDWMTAMLPISLLLM
                     LIGYLLAYKLFFPLKLEQRLPQIEGGMERLRQEYRSLGKIDLLEIKAVILFILILGFW
                     ATDRLHGISPTAVAFVGAIVALLPRIGIVNWNDVDIPWHLMLFSAGAYALGAGLSATD
                     LPVLAVNAFFDHAGIGDGTPFWAIFMLLTAVMIFSALLFQSKTMRCMIFVPIAIGVAS
                     RFGFSVISLALPVAFMIEHVYVLPFNSKPAALLYETDRYSMGDAFKFGFTMMVIAWLL
                     NIVAGETWFRILGITPNGVFGLF"
BASE COUNT         1795 a         2839 c         2811 g         1875 t
ORIGIN      
        1 gccgcggcga tgggcatcga aaacctgctc tgcctcaccg gcgaccacca gaaattcggc
       61 gaccatcccg aggcccgcgg ggtcttcgac cttgactcga tccagctcat cgccgcggtg
      121 cagcaaatga acgcgggcag cctgctctcg ggccaattcc tcaaaaaggc ccccgccttc
      181 ttcatcgggg cggcggccaa cccctttgct gagcccctcg agatgcgcct catccgcctc
      241 gagaagaagg tgcgcagcgg ggccgccttc atccagaccc agccggtctt cgatctcgac
      301 cagttcagcc gctggctcga gctggtgcgc gggcgcggcc tgcatgaaaa gagcgccatc
      361 ctcgccgggg tgctaccggt gcggtcggcc aaagccctgg agtatatggc cagggaggtg
      421 ccgggcatgc gcatcaatcc ccgctatctc gagcgcatgc gcacggctgc cgagccgcag
      481 gaggaggggg tggccatcgc tgtcgagatc atcggcgccc tgcgggccat ggcgggggtg
      541 gccggcatcc acctcatgcc ggtgatgtgg gagagcatca ccccgcgcat cattcaggag
      601 gccgggctca tgcccccgga agctcccgct cccgcctgcg cgccctgtgc cggcgaggag
      661 aacccgatca acccctagcg gaacggaaag gccatgtcaa ccgaatacaa gggctaccgc
      721 ggcgaagccc tggccgcact cgaaaatttc caggctccgg tctggagcga cgtccgggtg
      781 gttaccggga agggaagcta cagcggcatc gtcctgccgc gctccgaaac cgccgacccc
      841 cggcacatcg tcctcaagct gcgctcgggc tataatatcg gcatcgccgc cgcctccgtc
      901 accgccatcg aggttctggg ccgcaaggag gcccactaca agattccgga aaaagagttc
      961 cccttcgatc ccaagaagcc gcgcgtcaag ctgctcggca ccggcggcac catcgccagc
     1021 cgcctcgact accgcaccgg cgccgtcatc cccgccttct cgccgggcga actctacggc
     1081 tccgtccccg aactggccga cctctgcaac ctcgagaccg aaaagctcta tggggtcttt
     1141 tccgagaaca tggggcctga acagtggatc ggcaccgcac aggcgatcgg ccgcgagatc
     1201 gaaaaggggg tacagggcat cgtcatcggc cacggcaccg ataccatgca ccacaccgcc
     1261 gccatcctct ccttcatggt gcagaattcc ccggtgccca tcgtcatggt cggctcgcag
     1321 cgctcctcgg accgcccctc ctcggatgcc gccctcaacc tcatgcacag cgtcaagacc
     1381 gccgccgaat cggacatcgc cgaggtaatg gtctgcatgt tcggccccac ctccgatgtc
     1441 tacggcctcc tccaccgcgg cacgcgcgtg cgcaaaatgc actccagcta ccgctccacc
     1501 ttccgcacca tcggcgatat ccccctggct atggtcagcc gcgaccgcat caccccgctg
     1561 cgccaggact acaaacgccg ccgccacgat cgcgaagtga ccatcaatac tgcctttgag
     1621 gagaaggtga gcatcgtcta ctactatccc aacatgaaac ccgacatcat cgacgccctc
     1681 atcgacaacg gctatcgcgg catcgtcatt gccggcaccg ggctgggcca cgtcaacaag
     1741 cccctctatc ccgccctcaa acgggcccgg gagaagaaca tcgccgtcta tatgaccgtg
     1801 cagaccctct ggggctatgt gcagatgtat gtttatgata ccgggcggga catgatggaa
     1861 ctgggagtga ccccgatggc caacatgctg ccggaagtgg cctatgtaaa actggggtgg
     1921 gcgctgggac agaccagcga cctcaagcgg gtgcgcgaga ttatgtatac gccggtcaac
     1981 ggcgagatca ccgaacgaga accatcgaac ggctatctca tcttccaggg cggcatcccc
     2041 gaggtggagg agttcatttc gcagatcaag aaatagcggg ccgctgcccc cctccggctg
     2101 cgttatctca gccggcgcag ggcgcattgt ccatccggct gtgttccacg cgctcagccg
     2161 gcgttgagct cattcaggat ggtctcgatc tcggcgcgga aggattcagg ggccatcagc
     2221 acgccccaac ccttctgcat ctggaagatc ccgtcgtagg ccgagtcatg aaaggtgcgc
     2281 acgacatggg ggatctccct ctccctgagc gcctcggcga gcacctgggc ctcgatctca
     2341 ttcttcagtg ttacgatccg gcaaaattcc gtcatttcgt ccttccttcc tgttcagatt
     2401 ttccagtttg ctttgcaggg tgctgcgcgg gatcttgagc tgccgcgccg ccgcgctgat
     2461 attttgactg cattcccgca tggcccaggt gatgatctcc cgctccctgc tgaggatata
     2521 ctcttcgtac gagccgtagc cggctggacc ctccgtctgc tccggcgctg ctttttctgc
     2581 gcggcctcga tcccccatac tcccggccgg catatagtcc atctcctgag gcagggcgag
     2641 gaggcgctgg actgtgttct cgagctcgcg gacattgccc ggccagtcgt aggccatcat
     2701 cttctggagc tgttcggggg tgatctcgac cggcctggcc ccgcggtggt gcttggcgat
     2761 gaaatgatcg atgagcaggg ggatgtcctc gcgccgctcg cgcaagggag gcagggtcag
     2821 ggcgatgatg ttgatgcggt aatagagatc ctcgcggaag gtgttctccc gcaccatctg
     2881 caggaggttg gccttggtgg cgcagatgac ccggaaattg accggcacgg tctcctggcc
     2941 gccgaggggc tggatctcgt gctcctgcag gacgcgcaaa agcttgacct gcaggcgcat
     3001 gggcagatcg tcaatgtcat cgatgaagag ggtgccgtcg tgggcgcgtt cgatgtagcc
     3061 gagatgacgc ttgtgcgctc cggtgaaggc ccccttttcg tggccgaaga gctcgctctc
     3121 gagcaggctc tcgggcagca cggcgcagtt gaccgccaca aagggcccct tgccgcgttc
     3181 actgttctgg tggagaaagt gtgcgaccag ctccttgccg gtgccgctct ccccctcgat
     3241 caacacggtg tgatcggtgg ctgccaccga gcgcagggtt ttaaccagcc tttgcatcac
     3301 cgcggcgctg ccgatgattt cgcgcccttg gtaatgggtg atctgtttct tgagctggat
     3361 attctcgcgg attaccgaat ggagctgctg gatgttggcc agcatgctga gcagcttgtc
     3421 gggagagaag ggcttggtca gataatcgta ggccccgagc ttgagcgcgg ccacggcggt
     3481 ctcgactgtg gcaaagccgg tgatgacgat gaccgtgcag ctgttctcgc gcattttggc
     3541 cagcttgacc agttcgaggc cgttcaggtc cggcaggcgc agatcggtga tcaccacctc
     3601 gaaggagtat ttctccaggg cttcgcggcc ggctcgtccg gtttcgcagg gggtcacctg
     3661 atagccctgt ttggtcaggg tatcggtgag ggtgatgcgc gatatctttt cgtcttcgac
     3721 gaggagtatt ttcatttggc gccctgtatc ggcaaggtga tgtgaaaggt ggtaccgcgg
     3781 tagggcctgc tctggcaggt gatggtgccg ccgtggcttt cgatgatgcc gagggagacc
     3841 gagagtccca gtccggtccc cttgccctca tccttggtgg tgaagaaggg gtcgaagatc
     3901 ttgtcgaggt cccggctgtc gatgccgcag ccggtgtcgg cgatctcgag atggatgtgc
     3961 ccctctccat tccgggcggt ggtgatacgg atggtgccgc cttcctcctc ctccatgctg
     4021 tccatgctgt tgaggagcag gttcatgatc acctcctgga tgagatgggg atcggcgtgg
     4081 aggaggggca ggtcggactg gaactttttc tcgatgatga tctggttttt gctgagccgg
     4141 taggtgagca gggagagcac cttttcgatc tcgttgttga gctccatggc caccgtctgc
     4201 ttggcgtgct gccgtgaaaa gccgagcagc ttttgcacga tcagggcgat gtggtcgagg
     4261 gcctcatcgg ccagccccag atagcgcttg acctctggca ggttgcgggg ttcctgtgca
     4321 atggcgtaga ggcagctctt catgccgtgc aggggattgt tgatctcgtg ggccacgccc
     4381 gaggccaggc ggccgatgga ggcgagcttt tcggcatggt agatctggcg ctgggcattc
     4441 aggagctgct gccgcgactg ctgcaggcgc tgcttcatct tttcgaaatt gctcaccagc
     4501 accccgatct catcttcgcc tgccttcatc tgcgtggggg tgatgttatc gaggtcgaac
     4561 tgcttcatct cggtgacgag ccggccgaga gactgggtga cccggttgat gaaaaaccag
     4621 ataaaggcgg tgatgctgac gacaaagagg acggcgaggc cgaagatgag gaaaaagagg
     4681 cgctgcagcc gggcgcgggc gggctcgagg tcgaagccca tgtgaagctc gccccacgat
     4741 ttgctgtgaa tctgcagggg cagggcggtc tccaggatcc agccaaagga gcgggagcgg
     4801 taaatacgcg tccctgtcct gccggccgca ggcggaaggg cttccgcctc cccggcatcc
     4861 cccggatcgc tgctctcaaa gctgctgcgc accgcgacat ggccgtcccg gtcgtacatc
     4921 accagccatc tcacctggtt gtttttgccc aggaagttgt ggatctggtt ctcgagatag
     4981 ccctcattgg ggaggaggcc gctctcctga taaatcaggc cgtcgaggat ggaggcggcg
     5041 aaggtgcgcg ccacggcgag ggcgttctgg cgctggttct cgaggatgag gctgcgccac
     5101 tgataaagca ggatgatcga gatcatcatc gtcagcacga tcatcagaat gcccagaaag
     5161 aggacaatac gcgctttaag gctcctcatc atcgccgggg ctccttctcg agtgcggcaa
     5221 agatggcgtc gacctggtcg taatcgctct cctcggccgg cacaaagccc caggcgaatt
     5281 cgggatccca atccctgacc agagcccggt gatcgggccg cctcgcgtcg atccggagca
     5341 gggccttgac catcgcctca gtgatgagtg gatccagatc ccggcgcacg atgatcggcg
     5401 aacccgggat ctcgcgggag acagcgatga cgcggatccc cttgccggtg aactcggcgg
     5461 cgacccggtc tttcactacc ccggcatcga agcgtccgag caggacctga tagaccacag
     5521 tatgatgaaa gccgaaatag cgcaccgaat ccagatcgct gaccttcaga ccatggcgct
     5581 gcagctcgta gccaagcagc cagttgccgg agaaggagag cgcggagggc agggcgagac
     5641 gcttgccggc gagatcggag atcgagcgga tgggagaatc atcccgtgtg atcaccatgg
     5701 aatggaaata gggctggagc tggctgttga ggggcttgag gatgcagcgg atcggatggt
     5761 cccggcgcgc cttgagatag agataggtgc cgagaaaggc ggactgaatc tggccctgat
     5821 cgagttgggc gatggtctcc tcatagctgc tgctcagcct gagggcgaag atgatcccgg
     5881 tctcccggct cagatagtcc atgatcggct gatagccttc gtaaatcagg ttgggcgaaa
     5941 agcgggagat caccccgacg tggacggtat cccgcccccc agcggcggca agcggtgcgg
     6001 caggggggaa gacggtatcg ggcgcggtgg tgatatccat gaaattgcga tagaccagca
     6061 gggtgagggc caggaaaaaa atgaggatga ggaggatcag ggcgattcgg gatttcatca
     6121 tgattccgtg acaaggtttt cagtccggga aattacggca aactttgctc agaagcaagc
     6181 agattgtcga aaaaccggca gtccagatgc cgaaatatcg gcaaatcgac gatctttcgg
     6241 catttcagca gggtcaagac tgcggggtct tgctgcagga tacatataaa caatgagtta
     6301 caatattgta tttgctggca tgagctttgc aaatggacgg cctgtagcac ataccaagag
     6361 gaggagagta tatgcccgaa atcaaacccc gcttcgaatt ccgcgccttc gcccagagct
     6421 tcggcatcgt cgaggagaag atgcgcgcca tcagccccgt cgagcagatt cgcgaaagcc
     6481 ttgagatcta tatcatgtcg gccgggaaca acgagaacaa caccaagatc cgcaacgacc
     6541 tcatggacat caaggtcctg gtgcaagtgg cgaagggcct ggagcagtgg aatccgcgca
     6601 tgaaggggca gttcccgatg gcggtggaga tgatcaaaaa cgaggtcttt cccgctttcg
     6661 gtgtcgcgat gcccgaattc aagcgcgaga gctataccct ggcgcagtac atcaacgaga
     6721 tcatcaagcc ccatccggat cttgccgcgg tcaatgtttt caaacgccgc atgggtttca
     6781 ccatcaacgg ctgcatcgcc gagctggccg aggtctatgt caacggcgct cgcatcatga
     6841 ccgccaatct cgaatcgacc gatgtcgagg ccatccttgc ggccaaaaaa atgatcgccc
     6901 tcgaggattt tgagaacgtc aattatttgc tggcgatcaa gcgggtgatc ggcatggagc
     6961 cgctctccga cacatcgatg tacaaagcca ctttctaggc cggaccgacc ggcgcagcgg
     7021 ccgcgccggc ccaggcccgg tccgctccta accttcgaca aaatggagtt tctatggcca
     7081 acgaggtcga acgcaaattt ctggtcaagg gcgaattcgc tcccttcgcc cataaaaaga
     7141 cccgcatcat ccagggctat ctctcctccg tgcccgaacg cactgtacgc gtccgcgtca
     7201 agggcgacaa gggctttctc accatcaagg gcatcggcaa tgcctccggt gcaagccgct
     7261 atgagtggga aaaggagatc ccggtcagtg aggccgagga gctgctcaag atctgcgagc
     7321 cgggcgtgat tgacaaatgc cgctaccaga tcacagccgg aaaacatacc tttgaggtcg
     7381 acgaattcta cggtgaaaat cagggactgg tcgtcgccga ggtcgagctc tctgcggagg
     7441 aagagtcctt tattaaaccc gcatggctcg gcgaagaggt caccggcgac gtccgctatt
     7501 acaattcgat gctgatgaag accccataca ccaggtggtg attccatcca gggtatggtt
     7561 gatccgctcc gcccgtgagc agcggcgcga cacaaacgga gtccgtctac catgacgaca
     7621 ttcgatcccc tcgatatgca caattaccgc atcgagcagc tgccgaagcg ggcaaagggc
     7681 cgcttcgaga aggggcttgc tgcgacgggt ccttttctcg ccctggccgc ctttatcctg
     7741 ttcggctggc tcgtcaagct ccccttcctg cagaatatcg atgcggccat gctgacctca
     7801 gcccaggcca aggcggcttt tgccaagatc ggcgccgagg cctttatccg cagcaacgag
     7861 atgatgctgg ccatttttgc cgccagcctg atcctctgga tgaccgaggc catccccaac
     7921 tacctcacct cactcatcgt catcatcgcc ctggtgctta ccagcgttct gcccgaaaag
     7981 actgcttacg cccagctcgg ccacgaggtg atgtggctca acatcatgtc cttcgtgctg
     8041 gcgagcatgc tggtggcgac gggcgtggcc aagcgcttcg ccctctggtt catcctccgc
     8101 ttcggtaaaa atgccaaccg catttttctc agcttcatct tcatcaacct gctgctctcc
     8161 gcctttatct cggcgaccac agccaaggcg gccattctgc tgcccatttt catggtgatc
     8221 gccgccatct acggcgcgcg cggcggcgag gagaagaaca actttggccg cagcattgtc
     8281 atccagaatc ttttcgagat caacgtcggg gccagcgcct tcatgaccgg ttccggggcc
     8341 aatctcctcg cggtctcgct gatcgccggg gctatcggca cccaggtttt cttctccgac
     8401 tggatgacgg ccatgctccc gatctcactc ctgctcatgc tcatcggtta cctgctcgcc
     8461 tacaagctct tcttccccct caaactggag cagcggctgc cgcagatcga gggcggcatg
     8521 gagcggctcc gtcaggagta ccgcagtctg ggcaagatcg atctccttga gatcaaggcg
     8581 gtgattcttt tcattctcat cctcggtttc tgggcgacgg accgcctgca cggcatcagc
     8641 ccgacggcgg tcgcctttgt cggggcgatt gtcgccctgc tgccgcgcat cggcattgtc
     8701 aactggaacg atgtcgacat tccctggcat ctcatgctct tctcggccgg ggcctacgcc
     8761 ctcggagcgg gcctcagtgc gaccgacctg ccggtgctcg cggtcaacgc cttttttgac
     8821 catgccggca tcggcgacgg cacccccttt tgggcgatct ttatgctcct gaccgcggtg
     8881 atgatcttca gcgccctgct ttttcagtcc aagaccatgc gctgcatgat ctttgtgccc
     8941 atcgccatcg gcgtcgccag ccgcttcggc ttcagcgtga tcagcctggc cctgccggtg
     9001 gcctttatga tcgagcatgt ctacgtgctg cccttcaaca gcaagccggc ggctctgctt
     9061 tacgagaccg accgctattc gatgggcgat gcctttaaat tcggctttac catgatggtg
     9121 atcgcctggc tgctcaacat cgtcgcgggc gagacctggt tcaggatact cggcatcacc
     9181 ccgaacggag tttttggcct cttttaatca ttctgtgagg cggagtcatg tcactcttgt
     9241 ttaaaaagaa caagcagacc gaggcccaga tcgacgagtt tctcgacctg gtcatcaagg
     9301 gagggctcat cttcaagcag
//
LOCUS       DAOPWU010000119         9303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7448_length_9303_cov_5.047253, whole genome shotgun sequence.
ACCESSION   DAOPWU010000119 DAOPWU010000000
VERSION     DAOPWU010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7448_length_9303_cov_5.047253"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PKM23_07700"
     CDS             complement(<1..792)
                     /locus_tag="PKM23_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNY91396.1"
                     /translation="MKIVHLSDLHWGFSPERDALIEQRLGELSACTADHVVISGDLSQ
                     SGRSEEFAALRSLLLGSGCYHPDRLTVIPGNHDLFSFFFRDFNAGRDLYARWRRLPGA
                     AASLYRYGWREYEADLDRFHSFFSETFEGIFTLAGNPAISYPFIKLLGERAALIVLES
                     NRMLPQIRRNSACSNGWIDLDAAAAILAHPGLENRFRIVVLHHQLQAEEQVAARMGRL
                     YAAMTRLINRTALIDLLDRSGVDLVLHGHYHHLEQYTVGRSIPVVN"
     gene            complement(827..1243)
                     /locus_tag="PKM23_07705"
     CDS             complement(827..1243)
                     /locus_tag="PKM23_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91397.1"
                     /translation="MKVEPDYHMYYVAPPEELEEIADTVEELVGQLEGPQYHTVLRIL
                     ALRRPWRPRHTGWVNVLDQILHFAYAVIVFLPLIIWPSWYTAGLGGLLLGAIREWEQW
                     KELDLKIPMIPDRLQDILFFGVGAVVLYFIHDALFG"
     gene            1511..2818
                     /locus_tag="PKM23_07710"
     CDS             1511..2818
                     /locus_tag="PKM23_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNY91398.1"
                     /translation="MKIHRASALIILMLFSALPGWAALPRPIQAMADSISEARLIEHI
                     LQLERAGGHWSRVRHTAGNDSGVAYIFRQFEALDHLDYVELDTFSLQARPGFTTRVQS
                     NVIATIPGSSRPGRVFIIGAHHDCSGSRMGSEIWNREWATMRAPGADDNATGVAVLLE
                     LARLMSEPAFGFRPECTIRFIAFAAEESSPADYKNHNGSRRAAQISRAAGEEIAGMVS
                     IDMIGYNPYVDYQSVICNDLSEALGVQFRAARDSLGIDLTIALKKNPSATYSDHEPYW
                     TEGYPAICLIENAPPWDNNAFYRANPFYHTSDDSSGTVNYRLVRKVGQMTLAAVALLT
                     APATAVEVEPVAPPALFTLEQNFPNPFNPATVIRYRLPVAASVHLTVHDALGREVALL
                     AEGPQRAGSHEVRFDASGLPSGVYFCLLRAGSFTAARKMLRIQ"
     gene            3109..7773
                     /locus_tag="PKM23_07715"
     CDS             3109..7773
                     /locus_tag="PKM23_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNY91399.1"
                     /translation="MLEKGPERLILEITTPLIVPDTLAVSSGLNTSFASSPWPLLQDE
                     RFPALPSQTLLLDWPVQEVHLRILSAAPEEGFAVAPLVYSSDLPVTGASVEPLPWQRE
                     FRSGPAVPAEVVYAGAAGRQRLWMLRIYPWRYDARAAVLSRRASMRLELTGSGGLPAA
                     ERRPALAGAGVLAMASSAHLARSSSGDSGVFLAPGRVRLTVDEEGWYRVTGADLRKAG
                     IDLLEVDVEKLRLSCGGTAVPFFFNGGKNGQLDPGESIEFYGTGLRREAPAATPDLYQ
                     DPFSRSNVYWLDWEGEAGVRMEEEYSESPTSGSARIPYAFYQTVHVEQDNHFTTFDDI
                     SMADSLRDHWLYDSGLAAGHKRSYPFPLRYPDARSLLPVKVRVMLTGLTTAYTELHQA
                     AVFLNDRFAARGRGERQGIIDLQSQEVIGLLAASLSAENNTLTLVNEFDPTRTDYIAL
                     NWFEVTYPRLYRADEGWLEFTIPPEEEPGLFRFTIDGFADGAVEIFKLGASRIAGASV
                     TEATARDGLRSMHLQFLDTVPSHDVRYIAVAASAKKTPLRIEAVRRQWQPSSGGDIDY
                     IVIAPRAFLAEKALPQLMQHRRGQGHRAAGVAVEDIFDYLNHGRRSPLAIKAFLKWAA
                     ERWPLKYCLLAGDGSFQRTPVRGDTLDLVPVYMRQTLKYGAAASDFWYTLLEGEDEIP
                     DLCIGRLPARTPEQLEIVVDKIIDHETRAVEDKWHNRLLFVGGNTMVFRTKGLALADK
                     VPPAWSPAMLFTTADPGRSPDPFFGNTPELLDFIDEGCAVIGFHGHGGGAIWSDDGLL
                     GLEDVASLNNRGRYPLILSMTCFTGDFEQPVGENLAETMLFAPQKGTMAFLGAGGYGW
                     LENDDMLQAAIMSCLYENPEATLGELLTAGKIRYFSQYYGSDIARAEVNQYTIFGDPA
                     TRLRLPVLRTEAGIANPLLAAGDTLELSLHWPFAPGTAAVEFELERGLPISGGSLHFT
                     GSRTGHRLPLPAGSTGGTGIVRFHGVDGLGLQQTHGAARFSLEPAFFDSLRVLPAGQD
                     TLRLLVQLFSSTAPDSVFCLFRGDTLRMEQDGPGWYSLAVKSWWVPLACQFMALYDGG
                     AILSGQYNHDPAGRLNVEALPDRLSWGGEEQPVLFLPVMNWGNGSGMARIYLDYRQGG
                     DESWHPLSADTIAVRPYTGALASFPLYTSPGDLSIRFRIETGQAGVLETWTAVTPHHY
                     ALEAGAGFRFIAGPADTLRPDDRALCVASPRTVSMNSVFRVERLAPARLHEQPDFAAS
                     PAIPVYSIQFSRPEAVVDGVLFSCAVPPADDAPGAVESAALFRYAAATRKWVRLPAAV
                     EGSRLNASIRESGLYTILWAADDRPPEVDAAFDGRPSAANGFVNRNTTISLQLRDENG
                     IDAAPGRLHFMLDGRLLDGNSWALPDSIINGNLIPLSLRPELQPGPHTLAVTVSDCNG
                     NTMAAREFNFQVAEAFALRMLGNYPNPFAARTTFVYLLSSSADKLSLRIYTASGRLIR
                     EFNGASAEDPNPLSADYHEITWDGRDEEGYEAANGVYFYRLTARSGDKSEEITGKMAR
                     LR"
     gene            7803..8810
                     /locus_tag="PKM23_07720"
     CDS             7803..8810
                     /locus_tag="PKM23_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91400.1"
                     /translation="MRHGLFCMILLAAAPLGAQATRYAGAALDLGVGARPLALGAAAA
                     AMSGGAELFRYNPASLGLCVNREIGLMYAPTFGSISEPMATLHYLGGAFPLPGGGSIA
                     LHWTRFSVDEIPLYPRLSGGSFLDRLQDPSLRPDGAPEGYFKDVEDAFYLSFARSFRF
                     ELPLGWLYTDLAVEVPIGLNIKMLRQSLHESSASGLGIDLGMMVRFDLAQLLQLRRAG
                     QMSLGLSALDLTRTTLVWQNQREEKIGTALLWGVHYSQPLSFHRSRLNFFWTLREKEE
                     RNALYGVEYELGGAALRVGYNESGLTAGAGLRWRRMRVDYAFTSLDFEGVHRIGCAFV
                     F"
     gene            8829..>9303
                     /locus_tag="PKM23_07725"
     CDS             8829..>9303
                     /locus_tag="PKM23_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91401.1"
                     /translation="MKKALLVVGITAGVWMACDQEVTNPQPGIPVPRFVAAAADTAVE
                     ERGIDAVPEEDAIFLQWHRDPELAGFQLFRRFEGEKNFSLVAVLTARDSSFFDRVPTD
                     LRCYYFLRGLDEEERMGAPSDTVDYMLLAKAFDLQVTPGEPLLFSWSTGLMRPAFY"
BASE COUNT         1579 a         3094 c         2859 g         1771 t
ORIGIN      
        1 gttgaccacc ggtatgctcc ttcccaccgt atactgctcc agatgatgat aatgtccatg
       61 cagcaccagg tcaaccccgc ttctgtccag gagatcgatc agcgcggtgc gattgatgag
      121 tctggtcatg gcggcgtaga gccgccccat gcgcgcggcg acctgctcct ccgcctggag
      181 ctggtggtgg aggacaacga tcctgaatct gttttccagc ccgggatggg cgaggatcgc
      241 cgcggcggca tcgagatcga tccatccatt ggagcaggcg ctgtttctgc ggatctgcgg
      301 cagcatgcgg ttggattcga ggacgatgag ggctgccctc tcgcccagga gcttgatgaa
      361 ggggtaggag atggcgggat tgcccgccag ggtgaagatg ccctcgaagg tctcgctgaa
      421 gaaggagtgg aaccggtcca ggtccgcctc gtactcccgc cagccgtagc ggtagagact
      481 ggcggcggcg cccgggaggc ggcgccatct ggcgtagagg tcgcggccgg cattaaaatc
      541 cctgaaaaag aaggaaaaaa ggtcatggtt gccggggatg accgtcagcc ggtccgggtg
      601 gtaacagccg ctcccgagca gcagactgcg cagggcggcg aactcttcgc tgcggccgct
      661 ctggctgagg tctccgctga tcaccacatg gtcggccgtg catgcagaga gctcgccgag
      721 gcgctgttcg atgagggcat cacgctccgg ggagaatccc cagtgcaaat cggagaggtg
      781 gacgattttc atggtgtggt ccgaagggcg gcataggtca tgcgctctat ccgaagagag
      841 cgtcgtggat gaaatagagg acgacggcgc cgaccccgaa aaagaggatg tcctgcagcc
      901 ggtccgggat catcgggatt ttcaggtcga gttccttcca ctgttcccat tcccggatgg
      961 cgcccagcag cagcccgccc agtcctgccg tataccagga gggccagatg atgaggggca
     1021 ggaagacgat gacggcatag gcgaaatgga ggatctggtc gagcacattg acccagccgg
     1081 tatggcgcgg ccgccaggga cgccgcaggg cgaggatgcg gagcacggtg tggtattggg
     1141 ggccctccag ctggcccacc agctcctcga cggtgtcggc gatctcctcc agttcttcgg
     1201 gcggggccac ataatacata tgatagtcgg gttcaacttt catgggttct cctttgccag
     1261 gcggatttaa ttgggcacct atgtattgat acacattttc tgcggcccat gcaacaaaaa
     1321 aatgcggaat atgccggaag agggggaaac cctcgcggcg gagtggaggc gcataatctc
     1381 cttggttttt agctcataca tgcctatctt ttaaaaatgc atccgggttt tccctggctg
     1441 caggagtggc attttttcga gacgaccacg ccgggaatta cccggcttca tctgcgggag
     1501 ctgcaagaga atgaagatcc atcgggcatc cgccctgatt attctgatgt tgttttcagc
     1561 cctgccgggt tgggcggcac tgccccgccc catccaggcc atggccgact cgatctccga
     1621 ggcgaggctg atcgaacata tcctgcagct ggagcgggcc ggcgggcatt ggtcgcgggt
     1681 gcgccacacg gccggaaacg acagcggcgt cgcctacatc tttcgccagt tcgaggcgct
     1741 cgaccatctc gactatgtcg agctggatac cttcagcctg caggcgcggc ccggctttac
     1801 caccagggtg cagagcaatg tcatcgcaac catccccggg tcgagccggc ccgggcgggt
     1861 ctttatcatc ggcgcccatc acgactgttc cgggagcaga atgggcagcg agatctggaa
     1921 cagagagtgg gcgaccatgc gcgcccccgg cgcggacgac aacgccaccg gggtggcggt
     1981 cctgcttgag ctggcgcggc tgatgagcga acccgctttc ggcttcaggc cggaatgcac
     2041 gatacgcttt attgctttcg ccgcggagga gagcagccct gccgactata aaaaccataa
     2101 cggcagccgc agggccgccc agatctcgag ggcggccggc gaggagatcg ccggcatggt
     2161 ttccatcgac atgatcggct acaatcccta tgtcgattac cagtcggtca tctgcaacga
     2221 tctttccgaa gcgcttggcg tccagtttcg cgcagcccgg gacagcctcg gcatcgatct
     2281 gaccatcgcc ttgaagaaga acccctcggc cacctacagc gatcacgagc cctactggac
     2341 ggagggctac ccggccatct gtctgatcga gaatgccccg ccctgggaca ataacgcctt
     2401 ttaccgggcg aatccctttt atcacacctc tgatgattcc tccgggacgg tcaattaccg
     2461 cctggtgcgc aaggtcgggc agatgaccct ggctgcggtg gccctgttaa cggcgccagc
     2521 cacagccgtg gaggtggagc cggttgcgcc gcctgcgctc tttacgctgg agcagaactt
     2581 tcccaatccc ttcaatcccg caacagtgat ccgctaccgc ctgccggtcg cagccagcgt
     2641 ccatctcacc gtccatgatg ctctcggtcg cgaggtggcc ctgctcgctg aggggccgca
     2701 gcgggccggc agccacgagg tgcgcttcga tgcttctggt ttgccaagcg gggtctattt
     2761 ctgcctcctc agagccggtt cctttaccgc tgcgcgcaag atgctgcgca tccagtaggg
     2821 ccgaggctga agattctctt ccgcaatcgg gacctggtag aggtacttgt ttttatgggt
     2881 cgaaagcagg ccgggcgtcc gcgccgccgc aatactgcaa tgctcggccc gactgcaaat
     2941 tccatgcgat ggctgatcaa cgccttgcag cagctggccc cgagcctgtc ggtaacgcat
     3001 cctttgagcg ccatggtccc cgattctcac aaaaattgtt tgatacgctg cctgtttctg
     3061 ctggtctggt tcagtacgct tccggccccg gcccaggaat ggtgccgtgt gcttgaaaaa
     3121 ggtccggaac ggctgatcct cgagatcacc acccccttga ttgtaccaga caccctcgcc
     3181 gtctcttccg gcctcaacac ctccttcgcc tcctccccct ggcccctgct tcaggacgag
     3241 cgctttcccg cccttccctc tcagaccctg ctgctcgact ggccggtcca ggaggtccat
     3301 ctgcgcatac tctccgccgc ccccgaagag ggcttcgctg ttgcccccct ggtgtacagc
     3361 tccgatctcc cggtcaccgg cgcctccgtt gagcctttac cgtggcagcg cgaatttcgt
     3421 tccggccctg ccgtcccggc cgaggtggtc tacgccggcg cagccggccg gcagcggctg
     3481 tggatgctga ggatctatcc ctggcgttat gatgcgcggg ctgccgtgct gtcgcgccgc
     3541 gcctccatga ggctggagct gaccgggagc ggaggcctgc ccgctgcgga gcgccggccg
     3601 gcactggccg gagcgggggt gctggcgatg gcctcctccg cccacctggc gcgcagcagc
     3661 agcggcgaca gcggcgtttt tctcgcgccc ggccgcgtcc ggctcactgt cgatgaggag
     3721 ggatggtacc gggtcaccgg cgccgacctg cgcaaggccg gcatcgacct gctcgaggtc
     3781 gatgtcgaaa aactgcggct gagctgcggc ggaacagcgg tgcccttttt cttcaacggc
     3841 gggaagaacg gccagcttga tcccggcgag agcatcgaat tctacggcac cggcctgcgc
     3901 cgggaggccc cggcggccac ccccgacctc tatcaggatc ccttcagccg gagcaacgtc
     3961 tactggctcg attgggaggg cgaggcgggg gtccggatgg aggaggagta cagtgaaagc
     4021 cccacctccg gcagcgcacg catcccctat gccttttatc agacggtgca tgtggagcag
     4081 gataaccatt tcaccacctt cgatgatatc tcgatggcgg attccctgcg cgaccactgg
     4141 ctctatgaca gcggcctggc agccggccac aagcgcagct atcccttccc cctgcgctat
     4201 cccgacgccc gttcccttct gcccgtcaag gtgcgggtca tgctcaccgg attgacgaca
     4261 gcttataccg agctccatca ggcggcggtc tttctcaacg accgtttcgc cgcacgcggg
     4321 cgcggcgaac ggcagggcat catcgatttg cagagtcaag aggtcatcgg cctgctggcc
     4381 gcctccctga gcgccgagaa caacaccctc accctggtca acgagttcga tcccacccgg
     4441 accgactata tcgccctcaa ctggtttgag gtcacttatc cccgcctcta ccgggccgac
     4501 gaagggtggc tggaattcac catcccgccg gaagaggagc ccggactgtt ccgcttcact
     4561 atcgacggct ttgctgacgg agcggtcgag atcttcaaac tcggcgccag ccgcatcgcc
     4621 ggcgcaagcg tgaccgaggc caccgccagg gacggcctgc gctcgatgca cctccagttt
     4681 ctcgacaccg tgccctcgca tgatgtccgc tacatcgctg tcgccgcctc cgccaaaaag
     4741 acacccctgc gcatcgaggc ggtgcggcgg cagtggcagc cttcatccgg cggcgacatc
     4801 gattatattg tgatcgcccc gcgcgccttt ctcgctgaaa aagccctgcc gcagctgatg
     4861 cagcaccgcc ggggccaggg acaccgggcc gccggcgtgg cggtggagga tatttttgac
     4921 tatctcaacc acggccgccg cagtcctctg gccatcaagg cctttctcaa gtgggcggcg
     4981 gagcgctggc cgctcaagta ctgcctcctc gccggggacg gctcttttca gcgcaccccc
     5041 gtgcgcggcg acaccctcga cctggtgccc gtctacatgc gccagaccct caagtacggc
     5101 gccgccgcca gcgatttctg gtacaccctg ctcgagggcg aggatgagat ccccgacctc
     5161 tgtatcggcc gcctgccggc gcgcaccccg gagcagctcg agatcgtggt ggacaagatc
     5221 atcgaccacg agacccgcgc cgtggaggac aagtggcaca accgtctcct cttcgtcggc
     5281 ggcaacacca tggtcttccg gaccaagggg ttggctctcg ccgacaaggt gccgccggcc
     5341 tggtcgcccg caatgctctt caccactgcg gatcccggcc gttcgcccga tccctttttc
     5401 ggcaacactc cggagctgct cgatttcatc gatgagggct gcgcagtgat cggttttcac
     5461 ggccacggcg gcggggcgat ctggtcggat gacggattgc tcggactgga agatgtggca
     5521 agcctcaaca atcggggccg ctaccccctc atcctcagta tgacctgttt caccggggac
     5581 tttgagcagc ctgtgggcga aaatctcgcc gagaccatgc tttttgctcc ccaaaagggc
     5641 accatggctt ttttaggggc cggcggctat ggctggctcg aaaacgacga catgctgcag
     5701 gcggcaatca tgtcctgcct ctatgagaat ccggaggcca cccttggcga gctgctcacc
     5761 gccggcaaga ttcgctattt cagccaatat tacggcagcg acatcgcccg ggctgaggtc
     5821 aaccagtaca ccatcttcgg tgatcccgcc acgcgcctgc gcctgcccgt cctccgcacc
     5881 gaggcaggaa tcgccaatcc cctcctcgca gccggcgata ccctcgagct ctccctgcac
     5941 tggcccttcg ccccgggcac ggccgcggtc gaattcgagc ttgagagggg gctgcccata
     6001 tcaggcggca gcctccattt tacagggagc aggaccgggc accgcctgcc ccttccggcg
     6061 gggagcacag gcgggaccgg catcgtccgc tttcacggcg tggacggcct cggcctgcag
     6121 cagacccacg gggccgcccg cttttccctc gagccggcct ttttcgattc cctcagggtg
     6181 cttccggccg gccaggacac cttacgcctc ctggtgcagc tcttcagcag cacggcgccg
     6241 gattcggtct tttgcctctt ccgcggggat accctgcgca tggagcagga cggccccggc
     6301 tggtacagcc tcgctgtcaa atcctggtgg gttcccctcg cctgccagtt catggctctt
     6361 tatgacggcg gtgccatcct ttccggccag tacaaccatg atcccgccgg gcggctcaat
     6421 gtcgaggcct tgccggaccg cctgagctgg ggcggtgagg aacagcccgt actctttctg
     6481 ccggtaatga actggggcaa cggctccggc atggccagga tctaccttga ttaccggcag
     6541 gggggcgacg agagctggca tcccctctcc gccgacacca tcgcggtgcg tccctacacc
     6601 ggggccctgg cctcctttcc gctctatacc tcccccggcg acctcagcat ccgattccgc
     6661 atcgagaccg gccaggctgg ggtgctcgaa acctggacgg cggtcacccc gcaccattat
     6721 gccctcgagg ccggtgccgg ctttcgcttc atcgccggcc ccgccgacac cctgcgcccc
     6781 gatgaccgcg ccctctgtgt cgcctcacct cgaaccgtga gtatgaacag cgtgttcagg
     6841 gtggagcggc tcgccccggc ccggctgcac gagcagcccg atttcgccgc cagccccgcc
     6901 attccggttt acagcatcca gttcagccgc cccgaggcgg ttgttgacgg ggtgctcttc
     6961 tcctgtgccg tcccccctgc ggacgacgcg cccggcgccg tcgaatccgc cgccctcttt
     7021 cgttatgccg ccgccacccg caaatgggtc cgcctgcccg ctgccgtcga gggttcgcgc
     7081 ctcaatgcat cgatacggga atcgggcctc tacaccatcc tttgggcggc cgatgacagg
     7141 cctcctgagg tggatgccgc cttcgacggg cggccctctg cggcgaacgg ctttgtaaat
     7201 cgcaacacta ccatctcgct gcagctccgc gacgaaaatg gcatcgatgc cgccccgggc
     7261 aggctgcatt tcatgctcga cggccggctg ctcgacggga acagctgggc cctgcccgat
     7321 tcgatcatca acggcaatct gattccactc agcctgcgcc cggagctgca gcccggcccg
     7381 cacacccttg cagtgacggt cagcgactgc aatggcaaca ccatggcggc gcgcgagttc
     7441 aactttcagg tggccgaagc ctttgcgctc aggatgctgg gcaattatcc caaccccttc
     7501 gccgcgcgga ccacttttgt ctatctgctc tcgagcagcg cggacaagct ctcactccgc
     7561 atctacaccg cctccggcag actgatccgg gaattcaacg gcgccagcgc cgaggatccg
     7621 aatcccctca gcgccgacta tcacgaaatc acctgggacg gccgcgacga ggagggctac
     7681 gaggcggcca acggggtcta tttttaccgg ctgacagccc gttccggcga caagagcgag
     7741 gagataaccg gcaagatggc caggctgaga tgaaaagcgc acaacgacat cgcctgcggg
     7801 gaatgcggca cggcctgttc tgcatgattc tcctggcggc cgccccgctg ggggcgcagg
     7861 ccacgcgcta tgcgggggcc gcgctggatc tgggggtggg ggcccgcccc ctggccctgg
     7921 gagcggctgc cgcggccatg tccggcgggg cggaactctt ccgctacaat ccggccagcc
     7981 tgggcttgtg cgtcaaccgg gagatcggcc tcatgtacgc ccccaccttc ggttcgatct
     8041 ccgaaccgat ggctacgctc cactacctcg gcggggcctt tcccctgccc ggcggcggaa
     8101 gcatcgccct gcactggacc cgcttcagcg tcgacgagat ccccctctac cccaggctct
     8161 cgggcggctc ctttctcgac cggcttcagg atccttcgct gcggcctgac ggggcgcccg
     8221 aaggctattt caaggatgtg gaggacgcct tttatctctc cttcgcccgc tccttccgct
     8281 tcgagctgcc cctgggctgg ctctataccg atctggccgt cgaggtgccg atcggtctca
     8341 acatcaagat gctgcgccag tccctgcatg agagcagcgc cagcggtctg ggcatcgatc
     8401 tgggcatgat ggttcgtttc gaccttgcac agctcctgca gctccgccgc gcgggacaga
     8461 tgagcctggg gctctcggcc ctcgatctca cccgcacgac cctggtctgg caaaaccagc
     8521 gcgaggaaaa gatcggcacc gccctgctct ggggtgtgca ctacagccag ccgctctcct
     8581 ttcaccgcag ccgcctgaat tttttctgga ctctgcgaga aaaggaggaa cggaatgcgc
     8641 tctatggcgt ggaatacgag ctgggaggcg ctgccctgcg tgttggttat aatgaatcgg
     8701 ggctgaccgc cggagccggc ctgcgctggc ggcggatgcg cgtggattac gccttcacat
     8761 cgctcgattt cgagggcgtg catcgcatcg gctgcgcctt tgtcttttaa caagccggga
     8821 gatacgggat gaaaaaagcg cttctcgtgg tggggatcac ggcaggggtg tggatggcct
     8881 gcgaccagga ggtgaccaat ccccagccgg gcatccccgt gccgcgcttt gtcgcggcgg
     8941 cggccgatac ggccgtggag gagcggggca tcgacgccgt ccccgaggag gatgccattt
     9001 ttttgcagtg gcatcgcgac ccggagctgg cggggttcca gctcttccgc cgctttgagg
     9061 gggagaaaaa cttcagcctc gtcgcggtgc tgaccgcgcg ggacagctca ttcttcgacc
     9121 gggttccaac cgatctccgc tgctactatt ttttgcgtgg tctcgatgag gaggagagga
     9181 tgggtgcccc atcagacaca gtggattaca tgctcctggc caaagccttt gatctgcaag
     9241 tcaccccggg ggagccgctg ctcttctcgt ggagcaccgg cctgatgcgc ccggcctttt
     9301 atc
//
LOCUS       DAOPWU010000120         9180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7567_length_9180_cov_4.639781, whole genome shotgun sequence.
ACCESSION   DAOPWU010000120 DAOPWU010000000
VERSION     DAOPWU010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7567_length_9180_cov_4.639781"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PKM23_07730"
     CDS             <1..819
                     /locus_tag="PKM23_07730"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNY91402.1"
                     /translation="WAQEAAPDTTKPAPPWTHTLVGSLLGNQTTFTDWKQGGEDAIAW
                     NTLLEGKSVYDVGKDNWSTAYRFGFGNTKLGSKSTRKTDDRLEAETIYTWKLGRYVNP
                     YAAASFKTQFAPGYAYDANDVRTRVSKLFNPAYLTQTAGIGWQFLPQLKVRLGAGLRE
                     TLTSEDVFAAIYTDDKESADKIEKSVIEGGAESAVGVDWKLSKNLLLTSQVETFSPFN
                     DLAHSMLRTNTALAAAVSKYVTVRFNLITINEPRVTPRAQLKQALSFGLNYTFF"
     gene            complement(1023..3140)
                     /locus_tag="PKM23_07735"
     CDS             complement(1023..3140)
                     /locus_tag="PKM23_07735"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNY91403.1"
                     /translation="MKKTTIMMTGLILFALIFSLAPLASGQKHYKELQYGKLNDVTIP
                     QPKQVTLKNGIRLFLLEDHELPFIKMQTRFVGGSAWEPAEKAGLAGITGMVMRSGGSL
                     TMPGDRVDEELEKIAASVETGIGQLEGSASLSTLKEHFDKVLAIYNDILRNPAFPPEK
                     IELAKIEYKSGISRRNDDAGQIAQREYTQLIYGADSPYARDEEYATIDAITRDDLIAF
                     HKQYVQPKGMVVAVWGDFKTPEMLARLRKTFEGWKGAAAPLPKAPQVEYAFRKTVNLV
                     PRSDLNQSSIWLGHIGGLKNTPDEAALVMMNEILSGGFSSRLFNRLRATEGLAYNVSG
                     AYGTNILYPGIFYMQLQTKSARTVEAIQSMVREMRLMASEPVTEAELNYARESWLNAY
                     VFNFDSKDEVVRRMASYAFNGLPLDYLQQLRGKIEGVTVADVQRAAQKYLRPEEVQIL
                     VVGNPAEFGEPLSALGPVNEIDITIPVPGGQAVPEASGDALEKGKLAVSLMLEKMGGR
                     EKLAAVKSAEYIAKLAQSTPMGEMAMDAAITIVFPDKSCSIMKLPQGEIKMILNGDKG
                     MLVAPQGSMPAPEPVKNNMIENLFRDPFMLARQFGEVEMQYIGETTFADKPASEVIVS
                     KGKLNYHLFIDAAALLPLGVKYNTVGQTGPVEVEERYEDYRDVNGIQVAWKTLQYDKG
                     EKAAETGIVSVTLDGPVDMKLFE"
     gene            complement(3156..4643)
                     /locus_tag="PKM23_07740"
     CDS             complement(3156..4643)
                     /locus_tag="PKM23_07740"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNY91404.1"
                     /translation="MRKTMLFLLLLTAAGLQAQGYLDLQNQVVKHTLDNGLRLLILER
                     HEAPVVSFVTYADVGAVNESEGITGISHIFEHMAFKGTRTVGTTDLEAELKAMAKEDE
                     TFLAWRDEYAKGALADADKLKKLDEEHKAAVTAAKTYVVTNELGQAIEMAGGTGMNAG
                     TSYDQTVYFYNLPSNKVELWMSLESDRFLNPVLREFYTEKQVIMEERRLGVESQPVGK
                     LVEEFLAAAYKSHPYAEPVIGHMSDIRTISRQDAIDYFKTHYTPENLVLAVVGDVKAP
                     EVIRLAETYWGRLPKGPERRPLFTEEPPQLGQKRIEVEDAMQPILLLGYHRPSVYSKD
                     SAVLDAISDVLGSGRTCRLYKSLVKEKKIAVQVAAIANITEKYPSLYIFLLVPAQGHT
                     TAECEAAVLEEIDRIKKEPLTAEELTAVKTRAKAAFLGGLASNSGLAQQLADAEVRYA
                     DYRQMFTRVAKIEAIQTADIQRVAAEIFTKRNSTVAVIVPPAKAD"
     gene            complement(4662..5402)
                     /locus_tag="PKM23_07745"
     CDS             complement(4662..5402)
                     /locus_tag="PKM23_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNY91405.1"
                     /translation="MKSLILAAGYATRLYPLTKNFPKPLLPVGGRSILDRLLADLDAI
                     PVIDEHLIVTNATFYSQFERWRAEADYEKEIVLVDDTTTTNENRLGAVRDILHVIESL
                     GLHDDLLVLAGDNVVDFSFSGLAAFAREKGTSCITCHHEPSLPALQKTGVLVADSDFR
                     VEAIQEKPEAPASHWAVPPFYLYKAGDLPLIARALDEGCGHDAPGNLAAWLCRRTPMH
                     AWVLPGRRHDIGDLASYEAVNRLFTPSI"
     gene            complement(5421..6617)
                     /locus_tag="PKM23_07750"
     CDS             complement(5421..6617)
                     /locus_tag="PKM23_07750"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNY91406.1"
                     /translation="MLKHFHSLFPGTRKPDIRHVYSPYRVCPVGAHIDHQHGHVTGFA
                     LDHGVDLLYVPTETGVINLFSRNFDEQVLFPLETVPHKYSHWGRYVQAAIYALSKQTK
                     LTVGIQGLIAGSLPVGGLSSSAAVLLCYIMALADVNAIELDEHDLIRLAFEAEREYIG
                     LNLGKLDQSCEVLCRKDHLLYLDTKDDSYRLIPAGPAMPPFDILIFFSGLTRTLMQTG
                     YNTRTDECKVAAFDLLAYEGLPYGLLENTRLRDVDKGVFAKWQHMLPPPLAKRASHYM
                     SEFARVDEAVAAYQAGDIVRLGKIMFASGLSSIQQWESGCPEMIALYEIMCETPGIYG
                     GRFSGAGFKGCCVALGDPARRDEIIASVSARYLEKFPRLEGEYSAHVCKSADGARRIE
                     HHAVQV"
     gene            6958..8436
                     /locus_tag="PKM23_07755"
     CDS             6958..8436
                     /locus_tag="PKM23_07755"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNY91407.1"
                     /translation="MRVLKFGGSSVADGPRIGAMTAIAAEAAAGGRIAVVLSAMQGVT
                     DLLITAARKAAGGPEGGRIALEEIRGRHLAAVRHLFAAPDQSAVIAPLTIMLNELEEI
                     LHGIELIRECSPRTLDLVMSFGERLSCTLAAAHMRARGLDPLLLDARELILTDDSFGA
                     AVVDFQATCARIRHHFAAINGVVVIPGFIGATPAGVTTTLGRNGSDYTASLIGAGASA
                     EVIEIWTDVDGVLSADPRAVPDAFVVPEISYEEAMELSYFGARVLHPYTMLPAVEKGI
                     PIRIRNSRNPGAPGTLIGALRHRSGAPAARLITGIASIDGISLINIQGGGMIGVPGIA
                     ARVFSALARERINIIMISQASSEHTISLVLRSGEAARALAALDSELAMELTNRRIQAL
                     ERIDELLIVSVIGENMRGKPGLAGRLFTALGGAGVNILAIAQGSAERNISIVIGEKDH
                     TLALRAVHAAFLADPAAPAGTAAPAGPAARPAPDSAAGGGEA"
     gene            8436..>9180
                     /locus_tag="PKM23_07760"
     CDS             8436..>9180
                     /locus_tag="PKM23_07760"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNY91408.1"
                     /translation="MERIRVFAPATVANVASGFDVLGFALERPGDIVTLTRSDQPGVR
                     VTAIRGDGGRLPFDPAKNTAAVAAGALLEAIGRPFGLDIELDKGMPLASGLGSSAASA
                     VAAVTGANLLAGSPLSREQLLPFTLLSEEAACGSAHADNTAPALLGGFVLVRSYDPLD
                     VVRLPVPEGLACAVVHPHTEVRTEDARRILHKEIRLALAIRQWGNLAALVAALYRGDL
                     ELLGRSLQDVVAEPVRSVLIPGFDAVKAAA"
BASE COUNT         1523 a         3103 c         2839 g         1715 t
ORIGIN      
        1 tgggcgcagg aggctgcgcc cgacaccacc aaaccggcgc cgccatggac ccataccctg
       61 gtcggctccc tgctcggcaa ccagaccacc ttcaccgact ggaaacaggg cggggaggac
      121 gccatcgcct ggaacaccct cctcgaaggc aaatcggtct acgacgtcgg caaggacaac
      181 tggagcaccg cctaccgctt cggcttcggc aacaccaaac tgggctccaa aagcacccgc
      241 aagaccgacg accgcctcga ggctgaaacc atctatacct ggaagctcgg ccgctacgtc
      301 aatccctatg ccgccgccag cttcaagacc cagttcgctc ccggctatgc ctacgacgcc
      361 aacgacgtgc gcacccgggt ctccaaactc ttcaatccgg cctacctgac ccagacggcg
      421 ggcatcggct ggcagttcct gccccagctc aaggtgcgcc tcggcgccgg cctgcgcgaa
      481 accctcacca gcgaggatgt tttcgccgcc atctataccg acgacaagga aagcgccgac
      541 aagatcgaaa agtcggtcat cgagggcggc gccgagtcgg cagtcggcgt ggactggaag
      601 ctctcgaaga atctgctcct cacctcccag gtggagacct tctcgccctt taacgacctc
      661 gcccattcca tgctgcgcac caacaccgcc ctggccgcgg cggtgagcaa gtatgtgacg
      721 gtgcgcttta acctcatcac catcaacgag ccgcgggtca ccccccgcgc ccagctcaag
      781 caggccctct ccttcggact gaactatact ttcttctaga gagcgctcac caaataaaaa
      841 gccccgggct gatccccagc cactcgtttc cacccggccc ccttcacgtg ggcaccagcc
      901 gcagctcgct gattctgccg cccccccccc tccggtgccc acccggcccc cttcgggtgg
      961 gcaccaaata aaaaaagccc tgggctgaac cagcccaggg cttttgtttt tgtgcgcggc
     1021 ggctattcga acagtttcat atccaccggg ccgtcgaggg tgaccgagac gatcccggtc
     1081 tccgcggcct tttccccctt gtcgtactgc agggtcttcc aggccacctg aatcccgttg
     1141 acgtcacggt aatcctcata gcgctcctcg acctcaaccg gccctgtctg gccgaccgtg
     1201 ttgtacttga ccccgagcgg cagcagcgcc gccgcatcga tgaagaggtg gtagttgagc
     1261 ttgcctttgg agacgatcac ctcgctcgcc ggcttgtccg caaaagtggt ctcgccgata
     1321 tactgcatct cgacctcgcc gaactggcgg gcgagcatga agggatcgcg gaagaggttc
     1381 tcgatcatgt tgtttttcac cggctcgggc gcgggcatgc tcccctgcgg ggccaccagc
     1441 attcccttgt cgccgttgag gatcattttg atctccccct gcggcagctt catgatgctg
     1501 cagctcttgt ccgggaagac gatcgtgatg gcggcatcca tggccatctc gcccatcggc
     1561 gtgctctgcg ccagtttggc gatatattcg gcgctcttga ccgcggccag cttttcccgg
     1621 ccgcccattt tctccagcat cagcgagacc gcgagcttgc ccttctccag ggcatccccg
     1681 ctcgcctcgg gcaccgcctg cccgcccggc accgggatgg tgatgtcaat ctcgttgacc
     1741 ggcccgaggg cgctcagggg ctcgccgaat tcggccggat tgcccaccac caggatctgc
     1801 acctcctccg gccgcagata cttctgcgca gcccgctgca cgtccgccac cgtcaccccc
     1861 tcgatttttc cgcgcagttg ctgcagataa tccagcggca aaccgttgaa ggcatagctc
     1921 gccatgcggc gcacgacctc atccttgctg tcaaaattaa agacgtaagc gttgagccag
     1981 ctctcgcggg cataattcaa ttcagcctcc gtgaccggct cggaggccat caaccgcatt
     2041 tcgcgcacca tcgactggat cgcctcgacc gtgcgtgccg acttggtctg cagctgcatg
     2101 taaaagatgc cgggatagag gatgttggtg ccgtaggccc cggagacgtt gtaggccaga
     2161 ccctcggtgg cgcgcagacg gttgaagagc cggctggaga aaccgccgct gaggatctcg
     2221 ttcatcatca ccagggcggc ctcgtccggg gtgttcttca atccgccgat gtggccgagc
     2281 cagatgctgc tctggttgag atcgctcctg gggacgaggt tgacggtctt gcggaaggca
     2341 tactccacct gcggggcctt gggcaggggg gcagctgcgc ccttccagcc ctcaaaggtc
     2401 ttgcgcagcc tggcgagcat ctcgggggtc ttgaaatcgc cccagaccgc caccaccatg
     2461 cctttgggct ggacgtactg cttgtggaag gcgatgagat cgtcgcgggt gatggcgtcg
     2521 atggtggcgt actcctcatc gcgggcatag ggcgagtcgg ccccatagat gagctgcgta
     2581 tactcgcgct gggcgatctg gccggcatcg tcgttgcggc gcgagatgcc gctcttgtat
     2641 tcgattttgg ccagctcgat cttttcgggc ggaaaggcgg gattacgcaa gatgtcgttg
     2701 taaatggcca gcaccttgtc gaaatgctcc ttgagggtgg aaaggctcgc gctgccctcg
     2761 agctggccga tgccggtctc gaccgaggcg gcgatctttt ccagctcctc atccacgcgg
     2821 tcgcccggca tggtcaggct gccgccgctg cgcatcacca tgccggtgat gccggccagg
     2881 ccggcctttt cagccggttc ccaggccgat ccgcccacaa agcgcgtctg catcttgatg
     2941 aagggcagct cgtgatcctc gagcagaaag aggcggatgc cgttcttgag ggtcacctgt
     3001 ttgggctgcg ggatggtgac gtcgttgagt ttgccgtact gcagttcctt gtaatgcttc
     3061 tggccggaag cgagcggggc caggctgaaa atcagggcga agaggatcag ccccgtcatc
     3121 ataatggtcg tctttttcat tcctgatccc tttctctaat cggctttggc cgggggtacg
     3181 atgaccgcca cggtgctgtt gcgcttggta aagatttccg cggcgacgcg ctggatgtcg
     3241 gcggtctgaa tggcttcgat tttggcgaca cgggtgaaca tctgacgata gtcagcgtag
     3301 cgcacctcgg cgtcggcgag ctgctgggcc aggccgctgt tggaggcgag tccccccagg
     3361 aaggcggcct tggcgcgggt tttgaccgca gtcagctctt cggccgtcag cggttctttc
     3421 ttgatccggt caatctcctc aagcacggcc gcttcgcatt cagccgtggt gtggccctgc
     3481 gccggcacca acaggaaaat gtagaggctg ggatactttt cggtgatatt ggcaatggcg
     3541 gccacctgca cggcgatttt tttctccttc accaggctct tgtacaagcg gcaggtgcgg
     3601 ccgctgccga ggacgtcgct gatggcatcg agcacagccg agtccttgct gtagacgctg
     3661 ggacggtgat agccgagcag gaggatgggc tgcatcgcat cctcgacctc gatgcgcttc
     3721 tgcccgagtt ggggcggctc ttcggtgaag agcggacggc gctccgggcc tttgggcaga
     3781 cgcccccagt aggtctcggc cagccggatc acctcaggcg ccttgacatc gccgacgacc
     3841 gcaagcacca gattctcagg agtatagtgg gtcttgaagt agtcgatcgc atcctgacgg
     3901 ctgatggtcc tgatatcgga catgtggccg atcaccggct cggcataggg atgggatttg
     3961 taggcggccg ccagaaactc ctcgaccagc ttgccgaccg gctggctctc aactccgaga
     4021 cgcctctcct ccatgatgac ctgcttttcg gtgtagaact cgcgcagcac cggattgaga
     4081 aaacggtcgg attcgagcga catccacagc tccaccttgt tggaggggag gttgtagaaa
     4141 tagacggtct ggtcgtagga ggtgccggcg ttcatgcccg tgcccccggc catctcgatc
     4201 gcctgcccca gctcgttggt gacgacatag gttttggccg cggtcacggc cgccttgtgt
     4261 tcctcatcca gctttttcag tttgtccgca tcggccagag cccctttggc gtattcatcg
     4321 cgccaggcca gaaaggtctc gtcctctttg gccatcgcct tgagttcggc ctcgaggtca
     4381 gtggtgccga ccgtgcgcgt gcccttgaag gccatgtgct caaagatgtg cgagatgccg
     4441 gtgatgccct cggactcgtt gacggcgccg acgtcggcgt aggtgacgaa cgaaaccacc
     4501 ggggcctcgt ggcgttcgag gatgagcagg cgcagtccgt tgtcgagggt atgcttgacc
     4561 acctggttct ggaggtcaag gtagccctgg gcctgcaggc cggctgcggt gagcagcagc
     4621 agaaaaagca tggtttttcg catgactatc tccttggttg atcagataga tggtgtaaag
     4681 aggcggttca ccgcctcgta ggaggcgaga tcgccgatgt cgtggcgccg gccgggcagc
     4741 acccaggcgt gcatgggggt gcgccggcag agccaggccg ccagattacc cggagcgtcg
     4801 tggccgcagc cctcgtcgag ggcgcgggcg atgagcggca gatcgccggc cttgtaaagg
     4861 taaaagggag gcaccgccca gtgcgaggcc ggcgcctccg gcttttcctg gatcgcctcc
     4921 accctgaaat cggaatcggc gaccagcacc ccggtctttt gcagggccgg cagcgagggc
     4981 tcgtgatgac aggtgatgca gcttgtcccc ttctcccggg cgaaagcggc cagcccggaa
     5041 aaggagaaat cgacgacatt atcgccggcc agcacgagca ggtcatcgtg cagccccagg
     5101 ctctcgatga cgtggaggat gtcccgcacc gccccgaggc ggttctcgtt ggtcgtggtg
     5161 gtgtcatcga cgagcacgat ctccttctca taatcggcct ccgcccgcca gcgctcgaat
     5221 tggctgtaaa aggtggcgtt ggtgacgatg aggtgctcgt caatgaccgg gatggcatcg
     5281 agatcggcca gcagccggtc gaggatgctc cgtcccccca ccggcagcag gggcttgggg
     5341 aaattcttcg tcaggggata gaggcgggtg gcatagcccg cggccaggat cagggatttc
     5401 atcgtcgctc gccctggccc tcagacctga accgcgtgat gctcgatccg ccgcgccccg
     5461 tcggcgctct tgcagacgtg ggccgagtac tccccctcaa ggcggggaaa tttctccaga
     5521 tatctggccg agaccgaggc gatgatctcg tcgcggcggg ccggatcgcc cagggccacg
     5581 cagcagccct tgaagccggc cccgctgaag cggccgccgt agatgcccgg ggtctcgcac
     5641 atgatctcgt aaagggcgat catctccggg cagcccgact cccactgctg gatcgagctc
     5701 agccccgagg cgaacatgat cttgcccagc cgcacgatgt cgccggcctg ataagccgcc
     5761 accgcctcgt cgacgcgggc gaactcggac atgtaatgcg aggcccgttt ggccaggggc
     5821 ggaggcagca tgtgctgcca tttcgcaaaa acgcccttgt cgacgtcgcg caggcgggtg
     5881 ttctcgagca ggccgtaggg gagcccctcg taggcgagga ggtcaaaggc cgccaccttg
     5941 cactcgtcgg tgcgggtgtt gtagccggtc tgcatcaggg tgcgggtcag gccgctgaaa
     6001 aagatgagga tatcgaaggg cggcatggcc gggccggccg ggatgaggcg gtaagagtcg
     6061 tccttggtat cgaggtagag gaggtggtcc ttgcggcaca gcacctcgca gctctggtcg
     6121 agcttgccga ggttgaggcc gatgtactcg cgctcggcct cgaaggccag ccggatcagg
     6181 tcatgctcat ccagttcgat ggcgttgacg tcggccaggg ccatgatgta gcaaagcagc
     6241 accgcggccg aggaggagag gccgccgacg ggcagggaac cggcgatgag cccctggatg
     6301 ccgacggtga gcttggtctg cttggagagg gcgtaaatgg cggcctggac gtagcgcccc
     6361 cagtggctgt acttgtgcgg cacggtctcg aggggaaaaa gcacctgctc gtcgaaattg
     6421 cggctgaaga ggttgatgac cccggtctcg gtcgggacgt agaggaggtc gacgccgtgg
     6481 tcgagggcga agccggtgac gtggccgtgc tgatggtcga tgtgcgcccc gaccgggcag
     6541 acgcggtagg ggctgtagac gtggcggatg tcgggtttgc gcgtccccgg aaagagagag
     6601 tggaagtgtt tgagcatggt cggttcgcgt taggggtgag aggccgctgc ccggccgacg
     6661 ggtgcgggtg aataatctat tgttctaaag tttaaccatt attccgctct cattcaaggg
     6721 aaaaaatcgc ttcagctcct ttaacgtttt ctcgtgccca ccctggccgg cagcaggggt
     6781 tcaggtgggg tgtggcttgg tgtctttgcg gctttgtgag atctcctcct ggggtggcgg
     6841 cggggggctc tgcgtcttgg cgcctctgcg cgagcagttt tttctgcttc gttttccctt
     6901 gctatagtgg tgccgataaa ctatatttca cctttcaacc tcacgggaag ggaatctatg
     6961 cgagtactca aattcggcgg cagttcagtg gccgacggcc cccgcatcgg ggccatgacc
     7021 gccatcgccg cggaggccgc ggccggaggc cggattgcgg tggtcctttc cgccatgcag
     7081 ggagtgaccg acctgctcat caccgccgcg cgcaaggccg ccggcgggcc ggagggaggc
     7141 cgtatcgccc tcgaggagat ccgcggccgc cacctcgccg ccgtgcgcca cctcttcgcc
     7201 gcacccgacc agtcggctgt gatcgccccg ctcaccatca tgctcaatga actcgaggag
     7261 atcctccacg gcatcgaact catccgggaa tgctcccccc gcaccctcga tctggtcatg
     7321 agcttcggcg agcgcctctc ctgcaccctc gccgcggccc acatgcgcgc ccgcggcctg
     7381 gatcccctcc tccttgacgc ccgtgaactc atcctcaccg acgacagctt cggcgcggct
     7441 gtggtcgatt tccaggccac ctgcgcccgc atccgccacc acttcgccgc cataaatggc
     7501 gtagtcgtca ttcccggctt catcggggcc acccccgcgg gggtcaccac caccctcggc
     7561 cgcaacggct cggactatac cgcctcgctc atcggcgccg gggcctccgc ggaggtgatc
     7621 gagatctgga cggacgtcga cggcgtcctc tccgccgatc cacgcgccgt ccccgacgcc
     7681 ttcgtcgtcc ccgaaatcag ctacgaggag gcgatggaac tctcctactt cggcgcccgg
     7741 gtgctccacc cctacaccat gctgcccgcg gtcgaaaagg gcatccccat ccgcatccgc
     7801 aacagccgca atcccggcgc ccccggcacc ctcatcggcg ccctgcgcca tcgctccggc
     7861 gcccccgccg cccgccttat caccggcatc gcctcgatcg acggcatctc cctgatcaac
     7921 atccagggcg gcggcatgat cggcgtcccc ggcatcgccg ccagggtctt ttccgccctc
     7981 gcccgcgagc gcatcaacat catcatgatc tcccaggcct cctccgaaca caccatcagc
     8041 ctggtcctgc gcagcggcga ggccgcccgc gccctcgccg ctctcgactc cgaactcgcc
     8101 atggagctca ccaaccgccg catccaggcc ctcgagcgca tcgacgaact cctcatcgtc
     8161 tcggtcatcg gcgaaaacat gcgcggcaag ccgggcctcg ccggccgcct cttcaccgca
     8221 ctgggcggag ccggggtcaa catcctcgcc atcgcccagg gctcggccga gcgcaatatc
     8281 tccatcgtca tcggcgaaaa ggaccacacc ctcgccctgc gcgccgtcca tgccgccttc
     8341 ctcgccgacc ccgctgctcc cgccggcact gctgctcccg ccggccccgc tgcgcgcccg
     8401 gccccggatt ccgccgccgg cgggggagag gcctgatgga acgcatccgc gtcttcgccc
     8461 cggccaccgt ggccaacgtc gcctccggct tcgacgtcct cggcttcgcc ctcgaaaggc
     8521 ccggcgacat cgtcaccctg acccgcagcg atcaacccgg ggtgcgcgtc accgccattc
     8581 gcggcgacgg cggacgcctg cccttcgatc cggccaaaaa caccgccgcg gtcgccgccg
     8641 gggccctgct cgaggccatc ggccgcccct tcggactcga catcgaactc gacaagggga
     8701 tgcccctcgc ctccggactc ggcagctcgg ccgccagcgc tgtcgccgcc gtgaccggcg
     8761 ccaacctcct cgccggcagc cccctcagcc gggagcagct cctccccttc accctgctct
     8821 ccgaagaggc cgcctgcgga tcggcccacg ccgacaacac cgccccggcc ctgctcggcg
     8881 gctttgtcct tgtgcgcagc tacgaccccc tcgatgtcgt ccggctgccc gtgccggagg
     8941 ggctcgcctg cgccgtggtc catccccaca ccgaggtgcg caccgaggac gcccgccgca
     9001 ttctccacaa ggagatccgc cttgccctgg ccatccgcca gtggggcaac ctcgccgctc
     9061 tcgtcgccgc cctctaccgc ggcgacctcg aactcctcgg ccgctccctg caggatgtcg
     9121 tcgccgagcc ggtgcgatcg gtgctcatcc ccggcttcga tgcggtcaag gcggccgccc
//
LOCUS       DAOPWU010000121         9163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7586_length_9163_cov_4.362758, whole genome shotgun sequence.
ACCESSION   DAOPWU010000121 DAOPWU010000000
VERSION     DAOPWU010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7586_length_9163_cov_4.362758"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PKM23_07765"
     CDS             <1..996
                     /locus_tag="PKM23_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91409.1"
                     /translation="EERARATAAWVDDHRQPGDTPLGAELEFSQLGVRAIGARENADP
                     LFDGFYYFHDFDLARRGWKLGAHIDDHGFAAARGQRARGFLELAFGRYRLYGDVSKPA
                     TRDPWVLAQLIDLAARYIDVKPHSLHLSIQADENIPFKRLENPEYFICLLLLGGDLRE
                     DSSGRLREMRIFHGEILHPETGLHLSRLNRHHQRPGEESTLPVVEYQFARLHYEYDYQ
                     PLILALKGFQRRANPWPFKGCTGCPEQERHQDLETALKQWAAFPSPISQRCCDDFLEI
                     VRAGLEEEAAAAGERYTRYVHSHLEAIAAQLQRRNQRIHDYHQRLRRPEPVQGIT"
     gene            1062..2258
                     /locus_tag="PKM23_07770"
     CDS             1062..2258
                     /locus_tag="PKM23_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNY91410.1"
                     /translation="MQTEVIERAKEHFARIVTDQLARVERMKQGQEPADFASLKPIII
                     GFCWGDGIGPIISRETQRVLEYLLKEEIEKGKVDFRVIEGLTIENRVKHLKAIPDDVL
                     AELKACHVILKAPTTTPEKGGPYPNIESANVAMRRELDLFANVRPVKVPKDNIDWIFY
                     RENTEGAYILGSKGIQVTPDLAIDFTATTLQGSERIIRMAFDYAARNNINKVTVVTKA
                     NVIKTTDGLFLETAERIAKEYPAVKWDAWYIDIMTAKLIDPKRRTQFRVVVLPNLYGD
                     IITDEAAEFQGGVGTAGSANIGTRYAMFEAIHGSAPRMIDEGRGEYADPSSLIRAAAM
                     MLRHIGISDKAEQLEMALDICSQFEKEVVITGREDGATGRAFCDYLLETVKDPYLKKR
                     WQAQAR"
     gene            2321..3049
                     /locus_tag="PKM23_07775"
     CDS             2321..3049
                     /locus_tag="PKM23_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNY91411.1"
                     /translation="MNVALFIPCLSEHMFAESALSMVKVLDHFGIRIDYVEDQTCCGQ
                     PAFNSGYRKEIVPVAERLIRLFEGHEYVVAPSGSCTAMVRVFYRELEIDPALKPELES
                     LSHRIFEFTEFLVDVAKVATVDGSFPHRVTYHDSCHLLRELGVREQPRRLIRAISDID
                     FVEMEKPDTCCGFGGTFSYKMAEISLAMVERKCRYIEASGADYVVGADSSCLMNIDGW
                     LRKHNMHARGLHIADLLARSLGLA"
     gene            3273..4709
                     /locus_tag="PKM23_07780"
     CDS             3273..4709
                     /locus_tag="PKM23_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HNY91412.1"
                     /translation="MHTSPIHTKTYLHEALADSRLREAVDKATQTALKKRADKVEQLP
                     WWEELRHQAHQTKKETLDHLDRYLELFEENCQKNGIVVHWARDAEEARTIILELMREK
                     QVQSVVKSKSLTTEELDLNHFLSANHIESLETDLGEYIVQLLDQIPSHLTAPALHLTR
                     VDVGRIFHEKLGVPYTEDPDALLQIARARLREKFLSADMGISGVNFAMAREGSFCVVE
                     NEANGFLTVGLPRIHLAVMGIEKLIPDLASLPPFLKLLPVSATGQKATSYLHIIGGPR
                     RASTGEGPEEVHLLLLDNGRSRILADGQLRETLCCIRCGACLNVCPIYQQVGGHAYGW
                     VYMGPIGITLIPQYLGEAEGRYAPFLSSLCGACFEICPVRINIPHHLLKLRSRVVETG
                     NSGWMERLAFRGFGWLASHPRIYRIATWFPGKFQQLLPGERAFPVPGYGQKRALGRFD
                     GRGFRNRYNSWLRKRPQPSSSQEKEARS"
     gene            4706..5377
                     /locus_tag="PKM23_07785"
     CDS             4706..5377
                     /locus_tag="PKM23_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNY91413.1"
                     /translation="MTQAREAILARVRAALEHPSHLPEAPADTAARIAAQMAAATPAD
                     RRGLANQFKKEVETVSGECYILTSADAAADKLIELLELAPAAKLALDGDPLSAAVAAK
                     LKGTTHFDAAALPPEERRKRLPPVPAALVTADFGIADTGTLVVRFDAARSGWSHVLPE
                     TIYTLLPAGRLLPHLQALVERPEAGEAKNMVMITGPSRTADIEKILILGAHGPKRLVV
                     LIIME"
     gene            5450..8152
                     /gene="acnA"
                     /locus_tag="PKM23_07790"
     CDS             5450..8152
                     /gene="acnA"
                     /locus_tag="PKM23_07790"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNY91414.1"
                     /translation="MSPVTQDPFSAARKLDTAAGPVTIYALNALDRHAPVSRLPFSIK
                     VLLESLLRNCNGFDVTEADVTHLAGWNAAKPAEVEIPFKPARVIMQDFTGVPAVVDLA
                     AMRSAMQRMGGDPKKIEPLIPAELVIDHSVQVDTFGSREALARNMEIEFQRNRERYEF
                     LKWGQKAFETFKVIPPGVGIIHQVNLEYFARGVFTAVRPEGVVAYPDSLVGTDSHTTM
                     INGLGIVAWGVGGIEAEAVMLGQPLYMLMPEVIGFRLSGRLQPGATPTDIALTVTQML
                     RKKGVVGKFVEFYGSGLSQIALPDRALVANMAPEYGATMGFFPMDAEALAYLTRTGRS
                     RELVDLVERYSKEQGLFRSDATPDPVFTDTLELDLGTVEPSLAGPKRPQDRIPLPQVS
                     SAFKTTLSAPAEQRGFNLPADKLDKKVEIELEGQKVALEHGSVVIASITSCTNTSNAA
                     VMIGAGLLAKKAVGKGLKQKPWVKTSLAPGSRVVTAYLEKSGLLPYLEQLGFSVVGYG
                     CTTCIGNSGPLPEAVGAAVKGSELVAAAVLSGNRNFEGRVNALVKANYLASPPLVVAY
                     ALAGTMNLDLASEPLGTGTDGKPVYLQDIWPSPEELQAALRFADDPENYRAMYADVNA
                     FSPEWNEIPVKGGAIYEWQADSTYIQEPPFFVGLPAQPGAIQSIDKARVLALLGDSVT
                     TDHISPAGSIPAESPAGKYLISLGVQPKDFNSYGARRGNDRVMVRGTFGNIRLKNLLA
                     PGTEGGVTLHLPDGEKMWIYDAAMRYKAEGTALVVLAGKEYGTGSSRDWAAKGTLLLG
                     VRAVIAESFERIHRSNLVGMGVLPLQFREGDSAKNLGLTGHEEFTIRLDDNLKPRQEV
                     QVFARAADGSEIVFNTLCRIDTPVEVSYYRHGGILPAVLREMVK"
     gene            8167..>9163
                     /locus_tag="PKM23_07795"
     CDS             8167..>9163
                     /locus_tag="PKM23_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNY91415.1"
                     /translation="MSIIVNDQKLLRFVQEVFQSFGISAEKAFICADNLVLADLRGIP
                     SHGVARLKRYVDGMVNGTILPYAEPVVVREALSTATIDGQAGLGQVIAHFSTRMAIAK
                     AKQNGVGVVTVRNSNHYGIAGYYSQMMLEEGLLGLSMTNSAPLVVPTFGVEMIIGTNP
                     ISLTAPARRNRPFFLDMATSVVPRGKLEVYERLEKPMPEGWAVDAGGRVTTSAAEVLR
                     NMISRSGGGILPLGGEGELFSGYKGYGMAVLVDILCGVLSGGAYGDMVDSKGPSGENQ
                     PAQVAHFFMAINIENFVEMEVFQDKMDDLIDRLKASARAEGASRIFIHGEKEYELY"
BASE COUNT         1755 a         3244 c         2665 g         1499 t
ORIGIN      
        1 gaggagcgcg cccgcgccac agccgcctgg gtcgatgacc accgccagcc cggcgacacc
       61 cccctcggcg ctgaactcga attctcccag ctcggagtgc gcgccatcgg cgcccgggaa
      121 aacgcggacc ccctcttcga cggcttttac tatttccacg actttgacct cgcccgccgc
      181 ggctggaagc tcggcgccca catcgacgac cacggcttcg ccgccgcccg cggccagcgc
      241 gcccgcggct ttctcgaact cgccttcggc cgctaccgcc tctacggcga tgtctccaaa
      301 cccgccaccc gggatccctg ggtgctggcc cagctcattg acctggccgc ccgctatatc
      361 gacgtcaaac cccacagcct ccacctcagc atccaggccg atgaaaacat ccccttcaaa
      421 cggctcgaga atcccgaata cttcatctgc ctgctcttgc tcggcggcga tctgcgcgag
      481 gacagcagcg gccgcctgcg cgagatgcgc attttccatg gggagatcct ccatcccgag
      541 accggcctcc atctctcccg cctcaaccgc caccaccagc ggcccggtga ggagagcacc
      601 ctcccggtgg tcgagtacca gttcgcccgg ctccattacg aatacgacta tcagccgctc
      661 atcctcgccc tcaagggctt tcagcgccgg gccaatccct ggcccttcaa gggctgcacc
      721 gggtgccccg aacaggagcg gcaccaggat ctggagaccg ccctcaagca gtgggccgcc
      781 ttcccgagcc cgatttcgca gcgctgctgc gacgattttc tcgaaatcgt ccgcgccgga
      841 ctcgaagaag aggctgccgc cgccggcgag cgctataccc gctacgtcca cagccatctg
      901 gaggcgatag cggcccagct gcagcgccgc aaccaacgta tccatgatta ccaccagcgc
      961 ctgcgccggc ccgaaccggt gcagggcatt acctgacagc ccggccgccg ccacacgccg
     1021 gcagcgggga cccatgaacc gtattacaaa gaggagcaat tatgcagacc gaggtcatcg
     1081 aacgcgccaa agaacacttt gccaggatcg tcaccgacca gctcgcccgc gtcgaacgca
     1141 tgaagcaggg gcaggagccc gccgatttcg cctcccttaa gccaatcatc atcggctttt
     1201 gctggggcga cggcatcggc ccgatcatct cccgcgaaac ccagcgcgtg ctggaatacc
     1261 tcctcaagga ggagatcgag aagggcaaag tggactttcg cgttatcgag gggctgacca
     1321 ttgaaaaccg ggtcaaacac ctcaaggcca tccctgatga tgtgctggcc gaactcaagg
     1381 cctgccatgt cattctcaag gcgccgacca cgacgccgga gaaaggcggg ccctatccca
     1441 atatcgagag cgccaatgtg gccatgcgcc gcgaactgga tcttttcgcc aatgtgcggc
     1501 cggtcaaggt gccgaaggac aatatcgact ggatctttta ccgcgaaaac accgaggggg
     1561 cctatatcct cggcagcaaa ggcatccagg tcaccccgga tctggccatc gatttcaccg
     1621 ccaccaccct gcagggctcg gagcgcatta tccgcatggc cttcgactat gccgccagaa
     1681 acaacatcaa caaggtgacg gtggtgacca aggccaatgt gatcaagacc accgacggcc
     1741 tctttctcga aaccgccgaa cgcatcgcca aggagtatcc ggcggtcaaa tgggacgcct
     1801 ggtacatcga catcatgacc gccaagctga tcgatcccaa gcgccgcacc cagttccgcg
     1861 ttgtggttct gcccaacctt tatggcgaca tcatcaccga tgaggcggcc gagttccagg
     1921 gcggggtcgg cacggccggc agcgccaaca tcggcacccg ctacgccatg ttcgaggcca
     1981 tccacggctc ggccccgcgc atgatcgacg aggggcgcgg cgagtacgcc gatccctcca
     2041 gcctcatccg cgccgccgcc atgatgctgc gccacatcgg catcagcgac aaggccgaac
     2101 agctcgagat ggccctcgac atctgcagcc agttcgagaa agaggtggtg atcaccggcc
     2161 gcgaggacgg ggctaccggc cgcgccttct gcgactatct gctcgagacc gtcaaggatc
     2221 cctacctgaa gaaacgctgg caggcccagg cccgctgatc cggtccacag ctcccgcctg
     2281 ctgagcaggc gggagcgccc cttttcacac gaggagcact atgaacgtcg cccttttcat
     2341 accctgcctg tccgaacaca tgtttgccga atcggccctc tccatggtca aggtgctcga
     2401 ccatttcggc atccgcatcg actacgtcga ggaccagacc tgctgcggcc agccggcctt
     2461 caactccggc taccgcaagg agatcgtccc ggtggcggag cgcctcatcc gcctctttga
     2521 agggcatgaa tatgtggtgg cgccctccgg ctcctgcacc gccatggtgc gggtctttta
     2581 cagggagctg gagatcgacc cggccctcaa gccggagctc gagagcctca gccaccgcat
     2641 cttcgagttc actgaattcc tcgtcgatgt cgccaaggtg gccacagtcg acggctcctt
     2701 tccccaccgc gtcacctatc acgactcctg ccacctcctg cgcgagctcg gggtgcggga
     2761 gcagccgcgc cggctgatcc gcgccatcag cgacatcgat tttgtcgaga tggaaaagcc
     2821 cgacacctgc tgcggctttg gcggcacttt ttcctacaag atggccgaga tctcgctggc
     2881 gatggtggag cgcaaatgcc gctacatcga ggccagcggc gccgactatg tggtcggcgc
     2941 ggattcgagc tgtctgatga atatcgacgg ctggctgcgc aaacacaaca tgcacgcccg
     3001 cgggctgcat atcgccgatc tcctcgcccg cagcctggga ttggcataaa gattattctc
     3061 aaagatacaa acgcacaaag aatttgaaaa ataatctctc actgaggcac agaggcgcaa
     3121 agaaagaaag taaaaaccag tcaactttta tggtaatatg gcaaaaagag ctggcaatgc
     3181 ctgacttttt atctctgtgt cctccgtgcc cccgtgtgaa atttgctttg tgtccgttcc
     3241 gccgtttgat attgttaaat ttggagtgag tgatgcacac ctcccccatc cacaccaaaa
     3301 cctatctcca cgaagccctg gcggatagcc ggctgcgcga ggcggtcgac aaggcgaccc
     3361 agaccgccct caaaaagcgc gccgacaagg tggaacagct cccctggtgg gaggagctcc
     3421 gccaccaggc ccaccagacc aaaaaggaga ccctcgacca cctcgaccgc tatctcgagc
     3481 tctttgagga gaactgccag aagaacggca tcgtggtcca ctgggcgcgg gatgcggagg
     3541 aggcgcgcac catcatcctc gagctcatgc gcgaaaagca ggtgcagagc gtggtcaagt
     3601 ccaagtccct gaccaccgag gaactcgatc tcaaccactt tctcagtgcc aaccacatcg
     3661 agtccctgga gaccgacctc ggcgagtata tcgtccagct cctcgatcag atcccctccc
     3721 acctcaccgc gccggccctc catctcaccc gcgtcgacgt cggcaggatc tttcatgaaa
     3781 aactcggcgt gccctatacc gaggatcccg atgccctgct ccagatcgcc cgcgcccgcc
     3841 tgcgcgagaa attcctcagc gccgacatgg ggatctccgg ggtcaatttc gccatggccc
     3901 gcgaagggag cttttgcgtg gtcgaaaacg aagccaacgg ctttctcacc gtcggcctcc
     3961 cgcgcatcca tctggccgtg atgggcatcg aaaagctcat tcccgatctg gccagcctgc
     4021 ccccctttct caagctgctg ccggtcagcg ccaccgggca aaaggcgacc agctacctgc
     4081 atatcatcgg cggaccgcgc agggcatcga ccggcgaggg gcccgaggag gtccacctcc
     4141 ttctgctcga caacggccgc tcccgcattc tcgccgacgg ccagctgcgc gagaccctct
     4201 gctgcatccg ctgcggagcc tgccttaacg tctgcccgat ctaccagcag gtgggcggcc
     4261 atgcttacgg ctgggtctat atggggccga tcggcatcac ccttatcccc cagtatctcg
     4321 gcgaggccga ggggcgctac gcccccttcc tctccagcct ctgcggcgcc tgcttcgaga
     4381 tctgtccggt gcgcatcaac atcccccacc acctcctcaa gctgcgcagc cgggtggtcg
     4441 aaaccggaaa ctccggctgg atggagcggc tcgccttccg cggcttcggc tggctcgcca
     4501 gccatccccg catctatcgc atcgccacct ggttcccggg caagttccag cagctgctgc
     4561 ccggggagcg cgccttcccg gtgcccggct atggccagaa gcgcgccctc gggcgctttg
     4621 acggccgggg attccgcaac cgctataact cctggctcag aaaacggccg caaccaagct
     4681 catcccagga aaaggaggcc cggtcatgac ccaggcacgt gaagcgatcc tggcgcgggt
     4741 gcgcgccgcc ctcgagcatc cctcccacct gcccgaagct ccggcggaca ccgccgcccg
     4801 catcgcggcg cagatggcgg ccgcgacccc ggcggaccgc agggggctgg ccaaccagtt
     4861 caagaaggag gtcgagaccg tctcgggcga gtgttatatc ctgaccagcg cggatgcggc
     4921 ggccgacaag ctgatcgaac tgctcgagct cgccccggcc gccaaactgg ctctggatgg
     4981 cgatcccctc agcgccgcgg tggcggccaa gctcaagggg acgacccatt tcgatgccgc
     5041 cgccctcccg ccggaggagc gccgcaagcg cctgccgccg gtgccggccg cgctggtgac
     5101 cgccgatttc ggcatcgccg acaccggcac cctggtcgtg cgtttcgacg ccgcccgctc
     5161 gggctggagc catgtcctgc ccgagaccat ctacaccctg ctcccggccg ggcgtctgct
     5221 gccgcacctc caggccctgg tcgagcggcc cgaggccgga gaggcaaaaa atatggtgat
     5281 gatcaccggc cccagccgca cagccgacat tgaaaagatc ctcatcctcg gcgcccacgg
     5341 ccccaaacgc ctggtggtgc tgattattat ggaataaact accccaaacg gcggcaacgc
     5401 agtgcctccg tgccaccgtg tgaaaaataa taatctgaaa ggaaaaagca tgtcacccgt
     5461 aacacaagat cccttcagcg cggcgaggaa actggacacc gccgccggac cggtcaccat
     5521 ctacgccctc aacgcccttg atcggcacgc ccccgtctcc cgcctcccct tcagcatcaa
     5581 ggtgctgctc gaatccctgc tgcgcaactg caacggcttc gacgtcacag aggcggatgt
     5641 gacccacctc gccggctgga atgcggccaa acccgccgaa gtggagatcc ccttcaagcc
     5701 ggcacgggtg atcatgcagg atttcaccgg cgtccccgcc gtcgtcgacc tggcggcgat
     5761 gcgctcggcc atgcagcgca tgggcggcga tcccaaaaag atcgagcccc tcatccccgc
     5821 cgaactggtc atcgaccact ccgtccaggt cgacaccttc ggcagccgtg aagccctcgc
     5881 ccgcaacatg gagatcgagt tccagcgcaa ccgcgaacgc tacgagttcc tcaaatgggg
     5941 ccagaaggcc tttgagacct tcaaggtcat cccccccggg gtcggcatca tccaccaggt
     6001 caacctcgaa tactttgcgc gcggggtctt tacggcagtg cggcccgagg gcgtggtcgc
     6061 ctatcccgac agcctggtcg gcaccgactc gcacaccacc atgatcaacg gcctcggcat
     6121 cgtcgcctgg ggcgtcggcg gcatcgaggc cgaggcggtg atgctcggcc agcccctcta
     6181 catgctcatg cccgaggtga tcggcttccg cctcagcggc cgcctccagc ccggggcgac
     6241 gccgactgac atcgccctca ccgtcaccca gatgctgcgt aaaaaagggg tggtcggcaa
     6301 gttcgtcgag ttctacggca gcggcctctc gcagatcgcc ctgcccgacc gcgccctggt
     6361 cgccaacatg gcccccgagt atggcgccac catgggcttt tttcccatgg atgcagaggc
     6421 cctcgcctac ctcacccgca ccggccgttc ccgcgagctg gtcgatctgg tcgagcgcta
     6481 cagcaaggag caggggctct tccgcagcga cgcgaccccc gatccggttt tcaccgacac
     6541 cctcgagctc gacctcggca ccgtcgagcc cagtctggcc ggccccaagc ggccgcagga
     6601 ccgcatcccc ctcccccagg tgagcagcgc ctttaaaacg accctcagcg ccccggccga
     6661 gcagcgcggc ttcaatctgc ccgcggacaa gctggacaag aaggtcgaga tcgagctgga
     6721 agggcaaaag gtggccctcg agcacggcag cgtcgtcatc gcctcgatca ccagctgcac
     6781 caacacctcc aacgccgcgg tgatgatcgg cgccggactg ctcgccaaaa aggcggtcgg
     6841 gaaggggctg aagcaaaagc cgtgggtcaa gaccagcctc gccccgggat cgcgcgtggt
     6901 caccgcctat ctggagaaga gcggtctgct tccgtatctg gagcagctcg gattctccgt
     6961 ggtcggctac ggctgcacca cctgcatcgg caacagcggg cccctgcccg aggcggtcgg
     7021 cgcggcggtc aagggcagcg agctggtggc ggcggcggtg ctcagcggca atcgcaattt
     7081 cgagggccgg gtcaacgccc tggtcaaggc caactatctc gcctcgcctc ccctggtggt
     7141 cgcctacgcc ctggccggca ccatgaacct cgacctggcg tccgagccgc tcggcaccgg
     7201 cacagacggc aaacccgtct atctgcagga tatctggccc tccccggagg agctgcaggc
     7261 ggccctgcgc ttcgccgacg atccggagaa ttatcgcgcc atgtacgccg atgtcaacgc
     7321 cttcagcccg gaatggaacg agatcccggt caagggcggg gcgatctatg aatggcaagc
     7381 cgactcgacc tacatccagg agccgccctt cttcgtcggt ctgccggccc aacccggggc
     7441 gatccagtcc atcgacaagg cccgggtcct ggccctgctt ggcgactcgg tgaccaccga
     7501 tcatatctcc cccgccggat cgattccggc ggagagcccg gccggcaaat acctgatctc
     7561 gctcggggtg cagccgaagg atttcaactc ctacggcgcc cgccgcggca acgaccgggt
     7621 gatggtgcgc ggcaccttcg gcaacatccg cctcaaaaat ctgctcgccc ccggcaccga
     7681 gggcggcgtc accctccacc tgcccgacgg cgaaaagatg tggatctacg acgccgccat
     7741 gcgctacaag gcggagggaa cagccctggt ggtgctggcc ggcaaggagt acggcaccgg
     7801 ctcctcgcgc gactgggcgg ccaagggcac cctgctgctg ggagtgcgcg cggtcatcgc
     7861 cgagagtttt gagcgcatcc accgcagcaa tctggtcggc atgggcgtgc tgcccctgca
     7921 gttcagggag ggcgactcgg ccaaaaacct cgggctgacc ggccacgaag agttcaccat
     7981 ccgcctcgac gacaatctca agccgcgcca ggaggtgcag gtgttcgccc gcgccgccga
     8041 cggcagcgag atcgtcttca acaccctctg ccgcatcgac accccggtgg aggtgagcta
     8101 ctaccgccac ggcggcatcc tgccggcggt gctgcgcgaa atggttaaat aaaacgggag
     8161 gtgcccatgt ccatcatcgt caacgatcaa aagctgctcc ggttcgtgca ggaggtcttt
     8221 caatccttcg ggatcagcgc ggagaaggcc ttcatctgcg ccgacaacct ggtgctggcc
     8281 gacctgcgcg gcatcccctc ccacggcgtc gcccggctga agcgctacgt tgacgggatg
     8341 gtcaacggca ccatcctccc ctatgccgag ccggtcgtgg tccgggaggc tctctccacc
     8401 gccaccatcg acggccaggc cggcctcggc caggtcatcg cccatttctc cacccggatg
     8461 gccatcgcca aggcgaaaca aaacggggtc ggcgtggtca cggtgcgcaa ctcgaaccac
     8521 tacggcatcg ccggctatta cagccagatg atgctggagg aggggctcct cggcctctcg
     8581 atgaccaact ccgcccccct ggtggtgccc accttcgggg tggagatgat catcggcacc
     8641 aatccgatct ccctgaccgc gccggccagg cgcaaccgcc ccttcttcct cgacatggcc
     8701 acctcggtgg tgccgcgcgg caaactcgag gtctatgagc gcctcgaaaa gccgatgccc
     8761 gagggctggg cggtcgacgc cggcggacgg gtcaccacca gtgccgccga ggtgctcagg
     8821 aacatgatca gccgcagcgg ggggggcatc ctccccctcg gcggcgaggg cgaactcttc
     8881 agcggctaca agggctacgg catggccgtc ctggtcgaca ttctctgcgg cgtcctctcg
     8941 ggcggggcct atggcgacat ggtcgacagc aagggcccct ccggggaaaa ccagcccgca
     9001 caggtcgccc actttttcat ggccatcaat atcgaaaatt tcgtcgagat ggaggtgttt
     9061 caggacaaga tggatgatct catcgaccgg ctcaaggcct cggccagggc cgagggcgcc
     9121 agccggatct tcatccacgg tgagaaggag tatgaactct acg
//
LOCUS       DAOPWU010000122         9134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7618_length_9134_cov_4.772772, whole genome shotgun sequence.
ACCESSION   DAOPWU010000122 DAOPWU010000000
VERSION     DAOPWU010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7618_length_9134_cov_4.772772"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKM23_07800"
     CDS             complement(<1..726)
                     /locus_tag="PKM23_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91416.1"
                     /translation="MDRCQEGPRGKGILILIALLFCSALPGSAGTSGFQEYLVPGQES
                     LLRSHYVYLDDDPSVGSAMHCVISVTASTDGTIINYDHWENGFGTPDESYTVNTGQYK
                     IFESGNIPANPRGTATYYDGGDRITIIGGPAFVSRASWPESPGTVMALAFEILPIQAL
                     SNAFEMPVGSDLYQNDRSEPFADFERVVLMVQSTRDNNAVTVYGPDNAVIYSGTLNKG
                     QSAIDSDLRNVRKGTRVMATWPVQ"
     gene            complement(955..2316)
                     /locus_tag="PKM23_07805"
     CDS             complement(955..2316)
                     /locus_tag="PKM23_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNY91417.1"
                     /translation="MKRLLSYAGLLLLYIVGPARAQQPLTLDEVLRLALENNASIRNS
                     RLEGEAAEKIRIAAATRYFPGISAGGLYFDANRALFELRTPGGDLPVYDGNPANLLHP
                     TAFAYFPGGETSLLGRGQIGYLDIVQPLYAGGRIVNGNRLAALGRDAAADRSSLTRDD
                     IILATSEQYYRLLSLEEKRATLGSYAALLDRLIREVGDALDAGLILRNDLLKVRLKRS
                     EVRLDSSRLEHGRKLAAMALCQQIGIPRDSTLVLAGGWSTAAPPQSLYVEPESVLKSR
                     KEYALLEKGVRAARLQREMKRGELLPQAGIGARTMVVKLDEGPRRTLGIFYGSLSIPL
                     SDWIGGSAELEERRLREKIAENELQNSAELLLLQIEQAWQELSDGWTRIQLCEQAILQ
                     AEENLRVNEASFHSGLTALSELLEAQALLQKGRDEWTDARSEYMVKQCRYLQATGRSG
                     DLH"
     gene            complement(2313..5387)
                     /locus_tag="PKM23_07810"
     CDS             complement(2313..5387)
                     /locus_tag="PKM23_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNY91418.1"
                     /translation="MNKEKNTLLGLLLRYQQLLYLLVAIGILVGVAALVLMPRDEYPQ
                     FRMPLGIVVGIYPGASSEKVETQLTARVEQHLFQYKQVNRAKTYSVSKENVMAIYLHV
                     DGSEEELADFYSKLQDGLNSLKAELPSGVISLTADNDFGSTSALLLAVSSSSRSFKEL
                     ESHVKELEKDVRRVESVSRVKRYGAQREQISVYLDDARLARYGIKPLQVLAALKPPAS
                     VAYVGEIDDGHLTRPIHISSALATEIDVAGQIIWSDPQGTVLRVKDVARVVREYEEPA
                     STIRVNGEKCLIVSLEARPGKNIVAFGREVGEVTRDFAAGLPPDVKVTTISNVPFVVS
                     KSVGNFLKEFGISMLSVVAVTLLLLPGRVARIAALSIPASIFTALGIMWIWGLDLQTV
                     SLAGLILILGITVDDAMVVLDNYIEKLDHGMLPFEAGTKSVTELFSSVLSATLVIISC
                     FLPMPFFMTGTGEDFLRSLPLTITFALMVSLLVAVTLIPLASFHLIKSGISGRKTRRG
                     GRTFLDRMQSFYNGVVDKAFRHKKLVVATGILSLAAGLLLLATIPQQSFPKIERNQFA
                     VEVFLQPGSSLQQTDSVMRELEKILAADPRIEVIASFVGTSSPRFHTLYSPNYPARHY
                     GQMVVLTSSNRSTIEVLDEYSESLYNRFPNADIKWKQLSFDVQKAPIEVRISGDEIPE
                     LKRTGEQVAEIMRSIAGTEYIRTDWGQPLPTAELLLKQDEAARLGYSSALLGYSLMTG
                     TGGFPAATIWEGDYPVAVKIKTDKKVKSSPDDVLDQHVTSPLLAVSVPVRQIADLKPG
                     WSESDIVRRNGVRTLTVRCEVRRNLYPSRIFKELRPRVDRMTLPPGVSIAYGGDYQDT
                     RENMNPFYWSLATSIMLIFLILMAQYKRIRSVLLIMMTLPLSVFGAAFGVFLTGYPFG
                     VTAFVGVIGLMGIVVRNGVILVTYADELRAEGYTAEEAAMAAGKRRMRPIFLTSAAAA
                     VGVIPMILSGSTLWGPPAAAICSGLVFALALSLLIMPVLYYYFHRHQESAHS"
     gene            complement(5401..6441)
                     /locus_tag="PKM23_07815"
     CDS             complement(5401..6441)
                     /locus_tag="PKM23_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNY91419.1"
                     /translation="MKRGRITALFITASLLLAGCSGRDGQENITSAPVPVKAARPQPF
                     SAGHALAYSGTIEAGESIPLSFAAVGNVARVLVAEGEAVRKGQLLAELNAESSRNAWE
                     MSQAALKQAEDAWSRLKPMYESGSLPEIKWIEIETNLQKARSAAAISKKNLEDCRLIS
                     PVSGIVGRRAIDPGMGAMPNLASITIVRIDRVFAKVAVPENEIARVARGEQARLRISA
                     LEGAEYTGRVEEVGVVADPLAHSYAVRIAVENPGHRIKPGMICSAVLEDSSRSRGLVL
                     PAGAVLVDEKGINYLYVVNAESLAQRREVTIGALLQEGIEILSGLEPDELVVLAGQHK
                     LGTGKPVRVVTN"
     gene            complement(6461..7033)
                     /locus_tag="PKM23_07820"
     CDS             complement(6461..7033)
                     /locus_tag="PKM23_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY91420.1"
                     /translation="MSKDDIIREEIIAAAKRVFQKWGLKKATMEEIAAEAGKAKSTLY
                     YYYQSKEEIFDEAIFAHLAMILADVKAGLTAGLPVKEKIKRYVAASLTEMKHHAQIYS
                     IVWDEIKNNRRLLRKLRERLQQEERIFYREMLESGVQSGEFVFSSDKELIAAAHTLAG
                     LMRALILYLFLEAEESEQIDIAARLIANGI"
     gene            7267..7905
                     /locus_tag="PKM23_07825"
     CDS             7267..7905
                     /locus_tag="PKM23_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNY91421.1"
                     /translation="MKRYWFRAAAAALLFFIALPASAGLPFRPTTRLYMGISSPVAPQ
                     EFKDIHLSGWNIGGAIGKSFGERVELSLDGSYNAFSFDIQGYLNTLELKDQDEFESSA
                     DGGDASILTLFLHLKTKFPPQGDQRFVPYLFAEAGFFHFSQNEIHYWGPIGTDRSEPK
                     SSKTALGAGAGIGLNIAVEEHVAIFMDIGLKAGFTDGGTTSFFPFRFGVTFN"
     gene            complement(8063..>9134)
                     /locus_tag="PKM23_07830"
     CDS             complement(8063..>9134)
                     /locus_tag="PKM23_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91422.1"
                     /translation="IASTWSPGAPECIQAAPEPAQKRSSSPVPDPLVLVRVKEPNEGA
                     FSLLMPKGWISQGGIFYVDPNTTNGYANSVGPKGNFLVKRDQDGTVMIHWLPDFWYCD
                     TRYSPAGQMGLFPPGSYYNGMMVAPCPTAANFLLQFVFPQIRPDVTGFQLLATEPLPK
                     AAAKHRAQSAVPGAAYDAVRLTVRYTQNGVTYKEQMSCVIENLGQAAAGMWQNKETVC
                     VRAPLAEFSEWEKVGAMIYISARFDPNWVAAAARATAQRTQNAQATQRYIQDVDRQIV
                     ENRQRTNAEIRHEDYLMLTGQEDYVNPHTGETEIRPDGWKYHWENSNGEVIVSNLNDY
                     DPNHDDGVRLVKDFKRSQVRAR"
BASE COUNT         1817 a         2676 c         2821 g         1820 t
ORIGIN      
        1 ctggaccggc caggtcgcca tcacgcgggt acccttgcgg acattgcgca gatcgctatc
       61 gatggccgac tggcctttgt tgagagtccc ggaatagatc actgcattgt cgggaccgta
      121 gaccgtgacg gcgttgttgt cacgtgtcga ctgcaccatc aggaccacac gctcgaaatc
      181 agcgaaaggt tcggaacggt cattctgata gaggtccgat cccaccggca tttcgaaagc
      241 gtttgatagg gcctggatgg gcagaatctc gaaggccagg gccatcaccg tgcccgggct
      301 ttcaggccag ctggcgcgcg agacgaatgc cggaccgccg atgatggtga tacggtcgcc
      361 tccgtcgtaa taggtggccg tgccgcgtgg attggccggg atgttgcccg actcaaaaat
      421 cttgtactgt ccggtattaa cggtatagct ctcatccgga gtcccgaagc cgttctccca
      481 gtggtcataa ttgatgatgg tgccatccgt gctggcggtc acgctgatga cgcagtgcat
      541 cgcgctgcct accgatggat catcatcgag gtagacgtag tggctgcgca gcaggctctc
      601 ctggccggga acaagatact cctgaaaacc ggaagtaccg gcagagcccg gcagcgcgct
      661 gcaaaacagc aatgcgatga gaatgagaat ccccttgccc ctcggacctt cttgacacct
      721 gtccattttt cctctctcct ggttagagac tgtggcgccg agtaaacaac tttagctatt
      781 tcagaaatgc gataattcgc taactatata taataattaa ctatgcaaga aggatacccc
      841 cgaattaatt tcgctagatg caaggggaat gccagaaaat acaaaaagcc cgggccggcg
      901 aaataaagtt cttcgccata acccgggctt ttgacgccgg agcaaaaaga gggatcagtg
      961 caggtcgcca gacctcccgg tcgcctgcag ataacggcat tgtttgacca tgtattcact
     1021 ccgtgcatcg gtccattcat cccggccttt ttgaagcagg gcctgggcct cgagcagttc
     1081 ggagagagcc gtcaggccgc tgtgaaagct cgcctcattg acgcgcagat tctcctccgc
     1141 ctggaggatg gcctgttcgc agagctggat ccgggtccaa ccgtcgctca gctcctgcca
     1201 ggcctgttcg atctggagca gaaggagttc cgcactgttt tgcagctcat tttcagcgat
     1261 tttctccctg agtcggcgct cctcgagttc agcgctgccg ccgatccagt ccgacagggg
     1321 gatcgacagg gagccgtaaa aaattccaag ggtgcgacgc ggcccctcat ccagtttgac
     1381 taccatggtg cgcgcgccga tgccggcctg aggcagcagc tcgccccgct tcatctcacg
     1441 ctgcaatctg gccgctctca cccccttttc gagcagggca tactccttgc ggcttttaag
     1501 gacgctctcc ggctccacgt aaaggctctg cggaggagcg gccgtggacc agcctccggc
     1561 aaggacgagc gtcgaatcgc gggggatgcc gatctgctgg cagagagcca tggctgcaag
     1621 cttgcgcccg tgctccagac gggagctgtc gagccgcacc tcgctgcgct tgaggcggac
     1681 cttgagcagg tcgttgcgca ggatcaggcc ggcatcgagg gcatccccga cctcacggat
     1741 cagccggtcg aggagggcgg catagctgcc cagggtagcc cgtttctctt cgagggagag
     1801 cagccggtag tactgctcgc tggtggcgag gatgatgtca tcgcgggtca aactgctgcg
     1861 gtcggcggcg gcatcccgtc ccagagcggc gaggcggttg ccattgacga tccgtccgcc
     1921 cgcataaagg ggctgaacaa tgtccaggta gccgatctgc ccccgcccaa gcaggctggt
     1981 ctcccctccg gggaaatagg cgaaagcagt ggggtgaagc aggttggcag gattgccgtc
     2041 atagaccggc agatcgcccc cgggggtcct gagttcgaag agggcacggt tggcgtcgaa
     2101 atagaggccg ccggcactga tgccggggaa ataacgggtg gccgcggcaa tgcggatttt
     2161 ttctgcagcc tcgccctcga gccggctgtt gcgaatggag gcattgttct cgagggcgag
     2221 acggaggacc tcatcgaggg tgaggggctg ctgcgcacgg gccggcccca cgatgtacaa
     2281 gaggagcagc ccggcatatg acaataatcg tttcatgagt gggcactttc ttgatggcgg
     2341 tgaaagtaat aatagagcac cggcatgatc aggagcgaga gcgccagggc gaagacgagc
     2401 ccggagcaga tggcggcggc cggcgggccc cagagggtcg atccgctcag gatcatggga
     2461 atgacgccca ccgccgcagc ggcggaggtg agaaagatcg gccgcatccg tctttttccg
     2521 gcggccatcg ccgcctcttc ggcggtgtag ccctcagccc tgagctcgtc ggcataggtg
     2581 accagaatga ccccgttgcg tacgacgatg cccatcagtc cgatcacccc gacaaaggcg
     2641 gtgaccccaa aaggatagcc ggtgagaaag accccgaacg cggcgccgaa gacgctcagg
     2701 ggcagggtca tcatgatcag cagcaccgag cggatgcgct tgtactgggc catgaggatg
     2761 agaaaaatga gcatgatgct ggtggccagg gaccagtaaa aggggttcat gttctcccgg
     2821 gtatcctgat agtcgccgcc gtaggcgatg gagactcccg gcggcagcgt catgcggtca
     2881 acacggggcc gcagttcctt gaagatccgg gaggggtaga ggttccgtct cacctcgcag
     2941 cgcacggtca gggtgcgcac gccattgcgg cgcacgatat cggactcgct ccagcccggt
     3001 ttgaggtcgg cgatctggcg caccggcacc gaaaccgcca gcaggggcga ggtgacatgt
     3061 tggtcgagga cgtcgtccgg agaggacttg accttcttgt ccgtcttgat tttgacggcg
     3121 accggatagt ccccctccca gatggtggcg gccggaaagc ccccggtgcc ggtcatgagg
     3181 gagtagccga gcagcgcact ggagtagccc agacgggcgg cctcatcctg cttgaggagc
     3241 agctcggcgg tcggcagcgg ctggccccag tcggtgcgga tgtattcggt ccctgcaatc
     3301 gagcgcatga tctccgcgac ttgctcgcca gtccttttga gttcagggat ctcatcaccc
     3361 gagatccgca cttcgatcgg ggctttctgg acatcaaagg agagctgctt ccacttgata
     3421 tcggcgttgg ggaagcggtt gtagaggctc tcactgtact catccagcac ctcgatcgta
     3481 gacctgttcg agctggtcag aacaaccatc tgaccgtaat ggcgggccgg gtaattgggc
     3541 gagtagaggg tgtggaagcg gggcgaactg gtgccgacga aagaggcgat cacctcaatg
     3601 cgcggatccg cggcgagaat cttttcgagt tccctcatca cactatcggt ctgctgcagg
     3661 gaactgccgg gttgcaagaa aacctcgacc gcgaactggt tgcgttcgat cttgggaaaa
     3721 gactgctgcg ggatggtggc caggaggagc aggccggcgg ccagcgagag gatgccggtg
     3781 gcgacgacca gttttttatg ccggaaggcc ttatcgacca caccgttata aaagctctgc
     3841 atgcggtcga ggaaggtgcg cccgccgcgc ctggtcttgc gtccgctaat gccgctcttg
     3901 atgagatgga aactggccag cgggatcagg gtgaccgcga ccagcaagga gaccatcagg
     3961 gcaaaagtga tggtcagggg gagcgaacgg aggaaatcct cgcccgtacc ggtcatgaag
     4021 aagggcatgg ggagaaaaca ggagatgatg accagagtgg cggagaggac tgagctgaaa
     4081 agctcggtca ccgacttggt gcccgcctcg aagggcagca tgccgtggtc gagtttttcg
     4141 atataattgt cgagtacgac catggcgtcg tcgacggtga tgccgaggat gaggatgagg
     4201 ccagccaggg agacggtctg caggtcaagt ccccagatcc acatgatccc cagcgcggtg
     4261 aagatggaag ccgggatcga aagggcggcg atgcgcgcca ctcgccccgg taaaagcagc
     4321 agggtgacgg cgacgaccga gagcatcgag atgccgaact ctttgaggaa gttgccgacc
     4381 gatttggaga ccacgaaggg cacattggag atggtggtga ccttaacatc gggcggcagt
     4441 ccggcggcaa aatcccgggt cacctccccc acctcgcggc cgaaggcgac aatgtttttt
     4501 cccggccgcg cttccagcga gacaatcagg cacttctccc cgttgacgcg gatggtggat
     4561 gccggctcct catattcgcg caccacccga gcgacatcct tgacgcgcag aaccgtgccc
     4621 tggggatcgg accagatgat ctggccggcg acgtcgatct cggtggccag cgccgaggag
     4681 atgtggatcg gccgggtgag gtggccatca tcgatctccc cgacataggc gaccgaggcc
     4741 gggggcttga gggcggcgag cacctgcagg ggcttgatgc cgtaccgggc cagccgggcg
     4801 tcatcgaggt agacgctgat ctgctcccgc tgcgcgccat aacgtttgac ccgtgacacc
     4861 gattccaccc ggcgcacgtc tttttccagc tccttgacat ggctctcgag ttccttgaag
     4921 ctgcggctcg aggaactcac cgccagcagt aacgccgagg tgctgccgaa atcgttgtcc
     4981 gccgtcagcg agatcacacc cgaaggcagc tcggccttga ggctgttgag accgtcctga
     5041 agtttggaat agaaatcagc cagttcctcc tcggagccgt cgacgtgaag gtagatcgcc
     5101 atgacattct ctttcgagac agaataggtc ttggcgcgat tgacctgctt gtactggaaa
     5161 agatgctgtt caacgcgggc agtgagctgg gtctcgactt tttccgagga ggcgccggga
     5221 tagataccga ccacgatgcc gagcggcatg cggaactggg ggtactcgtc gcggggcatc
     5281 agcaccaggg cggcaacgcc gaccagaatg ccgatggcga cgagaagata gagcagctgc
     5341 tgatagcgca gcagcaaacc aagcagggtg ttcttttcct tgttcattct ctcccccgct
     5401 tcagttcgtg acgacacgga cgggcttacc cgtgcccagt ttgtgctggc cggcgagaac
     5461 gaccagctcg tccggttcaa ggccgctcag gatctcgatg ccctcctgaa gcagggcccc
     5521 gatggtcacc tcccgacgct gcgccaggct ctccgcgttg acgacataga ggtaattgat
     5581 ccctttttca tcaaccagca cggccccggc gggaaggacc agaccgcggc tgcggctcga
     5641 atcttcgagc acagcgctgc agatcatccc cggcttgatg cggtggccgg gattttccac
     5701 agcgatgcgg accgcatagc tgtgagcaag gggatcagcg acaactccga cttcctccac
     5761 cctgccggta tattcggcac cttcaagggc gctgatgcgc agacgggcct gctctccccg
     5821 cgcgacccgg gcgatctcat tttccgggac ggccactttg gcgaagaccc tgtcgatgcg
     5881 gacgatggtg atcgaggcca ggttgggcat cgctcccatg ccggggtcga tcgccctccg
     5941 gccgacgatc ccgctgaccg gggagatcag ccggcagtct tcgaggttct ttttcgagat
     6001 cgccgccgca gagcgcgcct tctggagatt ggtctcgatc tcgatccact tgatctccgg
     6061 caggctgccg ctctcgtaca tcggcttgag ccggctccag gcgtcctcgg cctgcttcag
     6121 ggccgcctgg gacatctccc atgcattcct gctgctttca gcgttgagtt cggccagcag
     6181 ctggcccttg cgcaccgctt ccccctcggc gaccagcaca cgggcgacgt tgcccacagc
     6241 ggcaaagctg aggggaatgg actcgcccgc ctcgatggtg ccgctgtagg ccaaggcatg
     6301 gccggcgctg aaaggctgcg gccgggccgc cttgaccggc acgggtgcgg aagttatatt
     6361 ctcctgacca tcgcggccgg aacaaccggc caggaggagg ctggccgtta taaacaaggc
     6421 agtaattcta cctcttttca tctactcatc tcctgtcaaa tcaaataccg ttggcaatca
     6481 gacgggcggc gatgtcgatc tgctcgctct cctcggcctc gagaaagagg tagaggataa
     6541 gagcccgcat caacccggcc aaagtatgcg cagcggcgat gagctcctta tccgaggaaa
     6601 agacgaattc acccgactgc acccccgact cgagcatctc ccggtaaaaa atccgctcct
     6661 cctgctgcag acgctcgcgg agcttgcgca gcaggcgccg attgttttta atctcatccc
     6721 agacaatgct gtagatctgg gcatggtgct tcatctcggt cagggaggcg gcgacatagc
     6781 gcttgatttt ttccttgacc ggcaaaccgg cggtgagacc ggctttgaca tcggcgagaa
     6841 tcatggccag atgggcgaaa atcgcctcat caaagatctc ctccttgctc tggtagtagt
     6901 agtagagggt gctcttggct ttgcccgcct cggccgcgat ctcctccatg gtcgcctttt
     6961 taaggcccca tttctggaag acacgcttgg ccgcggcgat gatctcctcg cggatgatat
     7021 cgtccttgct cattttcatc cttttcgact attttcgttt tatagtatga atttaatcat
     7081 tccagtggtt agaagcaaca attatttgcc ggaggcggct cgcctcgttt ctggctcccg
     7141 tacgcctcca tgtcaccttc gccaatccgg ctcgagttgt ttttacttgc attttaaccg
     7201 ggcaatggct attctatagc cgttccagca atgggattga cacggtttac caggcaggag
     7261 aaagttatga aaagatattg gttccgggct gcagctgcag ccctgctgtt ctttatagcc
     7321 ctcccggcct cggccggact acccttccgc ccaacgacaa ggctctacat gggcatatcg
     7381 agcccggtgg ctccgcagga gttcaaggat atacatctct cagggtggaa catcggcggc
     7441 gccatcggta agagtttcgg cgagcgggtg gaactgagtc tagatggcag ctacaacgcc
     7501 ttttccttcg atatccaggg ctacctcaac accctggaac tcaaggatca ggatgaattc
     7561 gaatcctcgg ccgatggcgg tgatgcctcc atcctcaccc ttttcctcca tcttaaaaca
     7621 aaatttccgc cccaaggcga tcagcgcttt gtcccctacc tcttcgccga ggccggattc
     7681 tttcatttca gccagaacga aatccactac tggggcccca tcggcaccga ccgcagcgag
     7741 ccgaaaagca gcaagaccgc cctcggggca ggggctggca tcggcctcaa cattgccgtg
     7801 gaggagcacg tggccatctt tatggatatc ggcctcaaag caggatttac cgatgggggc
     7861 acgacctcct ttttcccctt ccgctttggt gtgactttca actgaaccgg aatccgcagt
     7921 aataataaag cccgcgcagg catgcctgcg cgggcttttg cattcacagc tgcgaacaag
     7981 tgctagtgga ggatcggaat cttttcagcc ggacgcaaaa agttgccggt tccgaactcc
     8041 ttttccgctg ccagcgcccc gctcagcgcg cccgcacctg gctgcgtttg aaatccttga
     8101 ccaggcggac gccgtcgtcg tgattgggat catagtcatt gaggttggag acaatcacct
     8161 cgccgttcga gttttcccag tggtacttcc agccgtccgg ccggatctcg gtctcaccgg
     8221 tatggggatt gacatagtcc tcctggccgg tgagcatcaa gtaatcctcg tggcggattt
     8281 cggcgttggt gcgctgccgg ttttcaacga tctggcggtc gacatcctgg atatagcgct
     8341 gggtggcctg ggcgttctgg gtgcgctgtg cggtagcgcg cgcggcggcg gccacccagt
     8401 tcggatcaaa gcgggcggag atatagatca tcgcgccaac cttttcccat tccgaaaact
     8461 ctgcgagagg cgcccggacg cagacggtct ccttgttctg ccacatcccg gccgcggcct
     8521 gacccaggtt ctcgatgacg cagctcatct gctccttgta ggtcacaccg ttctgggtgt
     8581 aacggacggt cagccgcacc gcgtcgtagg ccgcgcccgg cacagccgac tgcgcccgat
     8641 gctttgctgc cgctttgggc agcggttcgg tggcgagcag ctgaaatccg gttacatccg
     8701 gacggatctg ggggaagacg aactgcagca gaaagttggc ggcggtaggg cagggtgcga
     8761 ccatcatgcc gttgtagtag gagcccggcg ggaagagccc catctggccg gccggactgt
     8821 agcgggtatc gcagtaccag aaatccggca gccaatggat catcaccgtc ccgtcctggt
     8881 cccgcttgac gagaaaattg cccttgggtc cgaccgaatt ggcataaccg ttggtggtgt
     8941 tgggatcgac gtaaaagatg cccccctggg agatccagcc cttgggcatg agcaaagaaa
     9001 aggcgccttc gttgggctct ttcaccctga ccagaaccag gggatcggga accggtgatg
     9061 acgatctttt ctgagcgggc tcaggtgcgg cctggatgca ttccggagcc ccgggagacc
     9121 aggtagaggc aatg
//
LOCUS       DAOPWU010000123         9114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7634_length_9114_cov_5.947676, whole genome shotgun sequence.
ACCESSION   DAOPWU010000123 DAOPWU010000000
VERSION     DAOPWU010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7634_length_9114_cov_5.947676"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PKM23_07835"
     CDS             complement(<1..591)
                     /locus_tag="PKM23_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNY91423.1"
                     /translation="MKAIDLRSDTVTKPTPEMRAAMAAAEVGDDVFGEDPTVNQLQAR
                     VAALLGKEAALYISSGTQSNQLAIRSHTQPGQEIYCEENGHIFNYEAGAPAALSGVQV
                     RTLPGVRGVITAAQIEAALRPADHHNPPSRLVVLENTHNRAGGAIFPFEEMEKIHALT
                     RERGLALHLDGARLWNAHVATGIPLLEYGRLFDSISV"
     gene            complement(604..3597)
                     /locus_tag="PKM23_07840"
     CDS             complement(604..3597)
                     /locus_tag="PKM23_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNY91424.1"
                     /translation="MRFIADLHIHSRYSRATSRQLIPEQLWRWGQLKGLQVIATGDIT
                     HPQWLAELEEALQPSAGCEGLFELKPAAAAAVKASVPPACQGNPLFILSGEISSIYKK
                     EGEVRKVHNVVYFPSFAAAALFQQRLERLGNIRSDGRPILGLDARDLLEIVLSTDPGA
                     QLIPAHIWTPWFSLLGSQSGFDSIQACFGDLTPHIFAVETGLSSDPPMNWRLSMLDGL
                     NLVSNSDAYSPEKLAREANIFNTELSYPALFAALETGGGERFAGTIEFFPEEGKYHMD
                     GHRKCGRMMRPDETRLHNGLCPVCGRPATLGVSYRVHELADRPEGSKPEGARPFHSLI
                     PLPELLGEVFDAGPATKRVQTLYHTLLRELGPELAILMERSPAEIEHTAGPMVAEAVR
                     RMRSGEVEAEPGYDGEYGIIRLFRLEERAGWLQQEALFRSESAPALPEKRQTAPPSPA
                     AAVKTATAKNLRAAEPPPGPWGLNQEQLEAVRHRGAPLIIQAGPGTGKTRTLTSRLAR
                     LLEEEFARPEEMLAITFTNKAAAGMRARLEEMLGTERAAPVRIMTFHAFGRSLLTAAG
                     AFFRRSPGFSILDTGADDRFRRALEARAGERLTATALERISALKGLLYTPDTLPEEIG
                     RSLPASFPALFRIYEELLVEWNAVDYDDLIALPVRLLRFEPELRRRQLEQNRVIAVDE
                     FQDIDAAQYELFRIFALHADDICVIGDPDQSIYGFRGASPEFFLRLQEELPGARLLRL
                     RQNYRSSRTILTASTGMLRSGAPAQGEETVAARTGKELKIQFHAAASDAAEAVFVAQQ
                     IEALIGGASHFALDADRADGERGHTYGFGDFAVLLRSRLLAGPLEEVLANSGLPFRRI
                     ADQYEKDRDDLTRPAGVSGQDESDEPSLPLDPRGDRIQILTLHAAKGLEFPVIFIIGC
                     EEELLPFAFGGSSDVEEERRLFYVGMTRARERLYLTHARTRMLRGQRRQQSPSRFLAD
                     ISRRLLQQHTAPQLRRKDKDQLKLF"
     gene            4012..5328
                     /locus_tag="PKM23_07845"
     CDS             4012..5328
                     /locus_tag="PKM23_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNY91425.1"
                     /translation="MKKTFVLDTNVILHDSSCIFYFQENDIVIPITVIEEIDAFKRGA
                     DLINYHAREFVRLLDTFPGDALVQDGVSLGEGRGKISILTLYDEEPEVNRAFLEQSPD
                     HRILSAALSLQKRGKASQVILVSKDVNLRLKARALGLESQDYTTDKIKDIDKLYRGKR
                     LIEEVDPEIINRFFAAPHQVPAAEIDIPNPIANEYFILRNASKSVLACYNPQTRMLER
                     VEKLQAYGISPRNAEQSFALNALLNPEIQLVTLSGKAGTGKTLLTLAAALERRREYRQ
                     IFLARPVVALSNKDLGYLPGDIESKLDPYMQPLYDNLGVIRNQFKESDERAQRINEML
                     ESGKLEIAPLAYIRGRSLDHIFFIVDEAQNLTPHEVKTIITRAGEGTKIVLTGDPSQI
                     DTPYLDSRSTGLNYLINRMHGQGMYGHVTLEKGERSPLAELASDLL"
     gene            5676..8801
                     /locus_tag="PKM23_07850"
     CDS             5676..8801
                     /locus_tag="PKM23_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91426.1"
                     /translation="MVLCSATWILAGTTGKITGVVLDSATGEGLPGANVLIKGTSMGA
                     VTDVNGHYTILNVPPGDYDLQVSFIGFNQVTVTGVRVNIDLTTNQNIRLKEQTLVGEE
                     ITVRAERPVIQKDLAASELQVTSDQIKNLPVSSVGEAIGLQAGVTSGLSIRGSGSEEA
                     LFMVDGISLRDERDNTPISKVPLSAVKQVSVQAGGFGAEYNNVRSGLVNVVTREGEAD
                     RYSATVTFRTNYRPWQKEDGYLKKYFGNVSPFGADSYWLRPYLDDAVAWTGTKNGAWD
                     EYTRRQYPEFDGWNAVSQTSLADDDPNNDVTPEAAQTIFKWQHRKQGDITKPDYVADF
                     GFGGPVPIIGKYLGNLRFFASARQDRDMYLFELSRADLFDRSSLLRLSADITPSMKLA
                     LSGMYGETFGTNYSLSGGTSIMSGTGDIAAQMTSAGFTVPTRIYTNIYFSPTSRFYST
                     LSSKLTHFINKSTYYDVQLKYQQKKYYTQPGGYRDETPKYEVYPGYFLDEAPYGFSVS
                     PVYGIDGDFMMGGAVGVSRDYSLYKNYSGKIDLVSQVNRRNQIKTGLELVYDDFNLSY
                     GQYNAYLPEGNVWTKVDQQPIRGNFYLLDKLEYEGFIATVGANLDYYTPNSQWYDVNI
                     YDRTFFAQGYTPDLEVQFKSKDVKPRWTLSPRMSISHPITENSKLYFNYGHYRQIPTS
                     ESLYRVQRTSINALSRYGDPTVPFEKTVAYELGYDHALFNNFLLHLAAYYKDITDQAY
                     SVRYISYDGKVNYYKNTNNSYEDIRGFEIDLSRTMGRWLVGDINYEYRVETSGYFGRA
                     YQYEDPAAQREYNKSNPVQSKPRPTPRLKAYLDFSTPQDFGALLGDWHATATASWTAG
                     WWGTWNPQDIAGILYNTQYKDYYNLNLKFSKTFYVGPMELKGFVDVYNALNFKRVSGY
                     GHVDTYDYNYYMKSLHLPASITRDLGYNAIPGSDRPGDYREDDVAFVPMEYVTDVNAI
                     AAPSTRAVYYEGPSERYMQYVDGQWQKVPEAELQQMLDDKAYIDMPNQSFLNFLNPRQ
                     IFFGITLNYNF"
     gene            8834..>9114
                     /locus_tag="PKM23_07855"
     CDS             8834..>9114
                     /locus_tag="PKM23_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91427.1"
                     /translation="MRVKNSLCWLALIAAILLVTIPVQADNTKWLATGMLHDWFSSGG
                     YEVEVGRRHLVADQQDGLQWPALFEYQDCKAAKALWIGTTNYNDPVAGKV"
BASE COUNT         1841 a         2715 c         2796 g         1762 t
ORIGIN      
        1 gaccgagatc gaatcgaaga ggcggccata ctcgagaagg gggatgccgg tggcgacgtg
       61 ggcgttccag agccgggcgc cgtcgaggtg gagggccaat ccgcgctcgc gggtcagggc
      121 gtggattttc tccatctcct caaaggggaa gatggccccg ccggcgcggt tgtgggtgtt
      181 ctcgagcacc accagccggg aggggggatt gtgatgatcg gccgggcgca gggcggcctc
      241 gatctgcgcg gcggtgatga ccccgcgcac gcccggcagg gtgcggacct gcactccgga
      301 gagagcggcg ggtgcgccgg cttcataatt aaaaatgtgg ccgttctcct cgcagtagat
      361 ttcctggccc ggctgggtgt ggctcctgat ggcgagctgg ttgctctggg tgccgctgct
      421 gatataaagg gcggcctcct tgccgagcag ggcggccacc ctggcctgga gttgattgac
      481 cgtcgggtcc tcgccgaaaa catcgtcgcc cacctcggcg gcggccatgg ccgcccgcat
      541 ctccggggtg ggtttggtaa cggtgtcgct gcgcaaatcg atggctttca tagaccttcc
      601 tgattaaaag agcttgagct gatccttgtc cttgcgccgc agctgcggcg cggtatgctg
      661 ctgcagcagc ctgcggctga tatccgccag aaaacgggag gggctctgct ggcggcgctg
      721 gccccgcagc atgcgggtgc gcgcatgcgt gagatagagg cgctcccgcg ccctggtcat
      781 gccgacatag aagagccggc gctcctcctc gacgtcggag ctgccgccga aagcgaaggg
      841 gaggagctcc tcctcgcagc cgatgataaa gataaccgga aattccagcc ccttggcggc
      901 gtgcagggtg aggatctgga tgcggtcgcc gcgcggatcc aggggcaggg aaggctcatc
      961 agactcatcc tgcccggaga cgccggccgg ccgggtgagg tcgtcgcggt ctttttcgta
     1021 ctgatcggcg atgcgcctga agggaaggcc gctgttggcg aggacctcct cgagcgggcc
     1081 ggcgagcagg cggctgcgca gcaggacggc gaaatcacca aaaccgtagg tatggccgcg
     1141 ctcgccgtca gcccgatccg cgtcgagagc gaagtggctg gcgcccccga tcagggcttc
     1201 aatctgctgc gccacaaaga ccgcctccgc cgcatccgaa gccgcggcgt gaaactggat
     1261 cttcagctcc ttcccggtgc gggcggccac ggtctcctcc ccctgcgcgg gggcgccgct
     1321 ccgcagcatt ccggtcgagg cggtgaggat cgtgcgcgag gaacgatagt tctgccgcag
     1381 gcgcagcagg cgcgcgcccg gcagctcctc ctgcaggcgg aggaagaatt cagggctcgc
     1441 cccgcgaaag ccgtaaatcg actggtccgg gtcaccgatg acgcagatat catcggcatg
     1501 gagggcgaaa atgcgaaaga gctcgtactg cgcggcatcg atgtcctgaa attcatcgac
     1561 ggcgatgacg cggttttgct ccaattggcg gcggcgcagc tccggctcga agcggaggag
     1621 gcgcaccggc agggcgataa gatcgtcata atcgacggca ttccattcta cgagcaactc
     1681 ttcatagatg cggaaaagag cggggaagga ggccggcagg ctgcggccga tctcctccgg
     1741 cagggtgtcc ggggtataca gcagtccctt gagggcggag atgcgctcca gggctgttgc
     1801 ggtgaggcgt tcgccggcgc gcgcctcgag ggcgcggcga aaacgatcat ccgccccggt
     1861 gtcgagaatg gaaaagccgg ggctgcggcg gaaaaaggcg ccagcggcgg tgagaaggct
     1921 gcggccgaag gcgtgaaagg tcatgatccg cacaggggcg gccctctccg tgcccagcat
     1981 ctcttcgagg cgtgcacgca tgccggcggc ggccttgttg gtgaaggtga tggccagcat
     2041 ctcttcaggc cgggcgaatt cctcctcgag cagcctggca aggcggctgg tcagggttct
     2101 ggtcttgccc gtgcccgggc cggcctggat gatgaggggg gcgccgcggt ggcgcaccgc
     2161 ttcgagctgc tcctgattga gcccccaggg ccccgggggc ggttctgcgg cccggagatt
     2221 ctttgctgtg gcagttttca ccgccgcagc aggcgaaggc ggggcagtct gccgcttttc
     2281 cgggagggcc ggggcggact cgctcctgaa cagggcctcc tgctggagcc agccggcacg
     2341 ctcttcgagc ctgaagaggc ggatgatccc gtattcgccg tcgtagccgg gttcggcctc
     2401 cacctcgccg ctgcgcatgc ggcgcacagc ctcggcgacc atcgggccgg cggtgtgttc
     2461 gatctcggca ggagaacgct ccatgaggat ggccagttcc ggccccagtt cgcgcagcag
     2521 ggtgtgatag agggtttgca cccttttggt ggcggggccg gcatcgaaaa cctcccccag
     2581 cagctcgggc aggggaatga ggctgtggaa gggtctggcg ccctccggtt tgctgccctc
     2641 ggggcggtca gccagttcgt ggacgcgata gctcaccccc agggtggcgg gcctgccgca
     2701 gaccgggcag agaccgttgt gaagacgggt ttcatcgggg cgcatcatcc ggccgcactt
     2761 gcggtggcca tccatgtgat acttgccctc ctcggggaag aactcgatgg ttccggcgaa
     2821 gcgctcacct ccccctgtct caagagcggc gaagagggct ggataggaga gctcggtatt
     2881 aaaaatattg gcttcgcggg cgagtttttc gggggagtag gcatcagagt tggagaccag
     2941 attgaggccg tcgagcatcg agagccgcca gttcatcggc gggtcggagg agaggccggt
     3001 ctcgacggcg aaaatatggg gggtcagatc gccgaaacag gcttgtatcg agtcgaagcc
     3061 ggattgggag ccgagcaggg agaaccaggg ggtccagata tgggccggga tcagctgggc
     3121 gccgggatcg gtggagagaa cgatctcaag caggtcgcgg gcgtcgaggc cgaggatggg
     3181 gcgcccgtcg gagcggatgt tgccgagtcg ttcgaggcgc tgctggaaga gggccgccgc
     3241 ggcgaaagag gggaaataga ccacgttgtg gaccttgcgc acctccccct ctttcttgta
     3301 gatgctgctg atctcgccgg agaggatgaa gagggggttg ccctggcagg cgggcgggac
     3361 cgaggccttg accgctgcgg ccgctgcggg tttgagttcg aagagtccct cacagccggc
     3421 ggaaggctgc agggcctcct caagctccgc gagccattgg ggatgggtga tgtcgccggt
     3481 ggcgatgacc tgcagtccct tgagctggcc ccagcgccag agctgctccg ggatcagctg
     3541 acggctggtg gcccgggagt agcgggagtg gatatgaaga tcggcgatga atctcatggg
     3601 gcaaggggcc tctgcggctt gatgatcggg gcaagcgttt acgggatgga gataatatac
     3661 aaatattttg ccaaagggac aacgcttttg cagggggcgg gccggccttg tccgcggtcc
     3721 gggtaatggg ctcgcggggg cgggaaaatg ggtctcctgc aaaagaccgt ggtaagttgg
     3781 ccagcaaaaa taactggaag cgattccaca accgccagcc cggtttcagg ggctgacagg
     3841 gatgaaaagg ggcccgggcg gcccttttat ccctccggct gaacgaggag ggcaaggttg
     3901 tttgcttatt aaaaataacc agaatatggt acttaaaggg cagaaagaac ttgcatattt
     3961 ttaattatta tattatttta agggacgaat ggagggcgat caggaggagg agtgaaaaag
     4021 acctttgtcc ttgataccaa tgtaattctg cacgattcca gctgcatctt ctatttccag
     4081 gaaaatgata ttgtcattcc gatcaccgtg attgaagaga tcgatgcctt caagcgcgga
     4141 gcggatttga tcaactatca cgcgcgcgag ttcgtccgcc ttctggacac ctttccgggg
     4201 gatgcgctgg tccaggatgg cgtcagcctc ggggagggac ggggcaagat ttccatcctc
     4261 accctttatg atgaggagcc ggaggtcaac cgcgccttcc tcgagcagag cccggaccat
     4321 cgcatcctga gtgcggccct ttcgctgcag aagagaggca aggcctccca agtcatcctg
     4381 gtcagcaagg atgtcaatct tcggctcaag gccagggcgc tcgggctgga gtcccaggat
     4441 tacaccaccg acaagatcaa ggacattgac aagctctacc gcggcaagcg cctgatcgag
     4501 gaggtggatc cggagatcat caacaggttc tttgctgcac cccaccaggt ccccgccgcc
     4561 gaaattgaca tcccgaatcc catcgccaac gaatacttca tcctccgcaa cgccagcaaa
     4621 tcggtactcg cctgttacaa tccccagacc cggatgcttg aacgtgttga aaagctgcag
     4681 gcctatggca tttcgccacg caatgccgag cagtctttcg ccctgaacgc cctgctcaat
     4741 cccgagatcc agctggtgac cctgagcggc aaggcgggca cgggcaagac cctgctcact
     4801 ctggctgcgg ccctggagcg gcggcgggag taccggcaga tctttctggc gcgcccagtg
     4861 gtggccctct ccaacaagga tctgggttat ctgcccggcg atatcgaatc caagctcgat
     4921 ccctatatgc agccgcttta cgacaacctc ggcgtcatcc gcaaccagtt caaggagagc
     4981 gacgagcggg cgcagcgcat caatgagatg ctcgagagcg gcaagctcga gattgcgccc
     5041 ctggcctata tccgcggccg cagtctcgat catatctttt ttatcgtcga cgaggcgcag
     5101 aacctcaccc cgcatgaggt caagaccatc atcacccggg ccggcgaggg gaccaagatc
     5161 gtcctgaccg gagatccctc gcagattgac accccctatc tggacagccg ttccacggga
     5221 ctcaattatc tgatcaatcg catgcacggt caggggatgt acgggcatgt caccctcgaa
     5281 aagggggagc gttcccctct ggcggagctg gccagcgatc tgttataaca aaatagttgc
     5341 ttttgtacgg gaaaattggt agactacaat ggtttagccg tatgctcctt tgtggccgcc
     5401 agtcccgcgc tgaaatactg cctttttctg tgagtagcaa actaccttcc ggtgcaggtg
     5461 gaggtaatca ggatgtactc atcagtctca cccgttatgg tcttctcatt aatggaattt
     5521 gcatgttttt aatagtgaaa tgcatgacgc ccggcatcat cacccatggt taagctcgtt
     5581 ttgacgcgcc gattgcctga tataccctca catcaacttt gtccccaaaa gaggagaaaa
     5641 ccgttatgaa gcttcatcgg ctaatgattt tgttgttggt cctgtgttcc gcgacctgga
     5701 tcctggcggg gacgaccggc aagattacag gcgtggtgct ggattctgcc accggcgagg
     5761 gattgcccgg cgccaatgtg ctgatcaagg ggaccagcat gggagcggtg accgatgtca
     5821 acggccacta taccattctg aatgtccccc cgggagacta tgatctgcag gtttccttca
     5881 tcggcttcaa tcaggtcacc gtcaccgggg tcagggtcaa catcgacctg accaccaacc
     5941 agaacatccg cctgaaggag cagaccctgg tcggcgaaga gatcacggtg cgcgccgagc
     6001 ggccggtgat tcagaaggac ctcgccgcca gcgagctcca ggtcacctcg gaccagatca
     6061 agaacctgcc cgtctcctcg gtcggcgagg cgatcggcct gcaggccggc gtcacttccg
     6121 gactctccat ccgcggcagc ggctcggaag aggccctctt catggtcgac ggcatctccc
     6181 tgcgtgatga acgcgacaac actccgatct ccaaggtccc cctcagcgct gtcaagcagg
     6241 tctctgtgca ggccggcggt ttcggggctg aatacaacaa tgtccgttca ggtctggtca
     6301 acgtcgttac ccgcgagggc gaagccgacc gctacagcgc caccgtcacc ttcaggacca
     6361 attaccgtcc ctggcagaag gaagacggct atttgaaaaa gtatttcggg aatgtttcgc
     6421 ccttcggcgc cgattcatac tggctgcgtc cctacctgga tgatgccgtc gcctggaccg
     6481 gcaccaagaa cggggcctgg gatgaataca cccggcggca gtatcccgaa ttcgacggct
     6541 ggaatgccgt ctcccagacc tccctggccg atgacgatcc gaacaatgat gtcacccccg
     6601 aagcggctca gaccatcttc aaatggcagc accgcaagca gggcgacatc accaagcccg
     6661 attacgtcgc cgatttcggc ttcggcggtc cggtccccat catcggcaag tatctcggca
     6721 acctgcgctt cttcgcctct gcccgtcagg accgcgatat gtacctcttt gagctctccc
     6781 gggccgatct cttcgaccgc agcagcctgc tcaggctgag cgccgacatc accccctcga
     6841 tgaagctcgc tcttagcggc atgtacggcg agacctttgg caccaactac agcctctccg
     6901 gcggcacctc gatcatgagc ggcaccggcg atatcgcagc ccagatgacc tcggccggtt
     6961 ttacggtgcc gacacgcatc tacaccaaca tctatttctc gccgacctcg cgtttctact
     7021 cgaccctctc ttccaagctg acccatttca tcaacaagag cacctattac gatgtgcagc
     7081 tgaaatacca gcagaaaaag tactacaccc agcccggcgg ttatcgcgac gagaccccca
     7141 agtatgaagt ctacccgggt tactttctcg atgaagcccc ctacggcttc agcgtcagcc
     7201 cggtctacgg catcgacggc gatttcatga tgggcggtgc ggtcggcgtc tccagagact
     7261 acagcctcta caagaactac agcggcaaga tcgatctggt cagccaggtc aaccgccgca
     7321 accagatcaa gaccggcctc gaactggtct atgatgattt caatctctcc tacggccagt
     7381 acaatgccta tctgcccgag ggcaacgtct ggaccaaggt cgaccagcag cccatccgcg
     7441 gcaattttta cctcctcgac aaactcgagt atgagggctt tatcgccacg gtcggcgcca
     7501 atctcgacta ttacaccccc aacagccagt ggtacgatgt caatatctac gatcggactt
     7561 tcttcgccca gggctatacc ccggatctgg aggtccagtt caaatccaag gatgtcaagc
     7621 cgcgctggac cctcagcccg cgcatgagca tctcccatcc catcacggaa aactccaagc
     7681 tctatttcaa ctacggtcat taccgccaga ttcccacctc ggagagcctc tatcgcgtgc
     7741 agagaacctc catcaacgcc ctgagccgct acggcgatcc gaccgtgcct tttgaaaaga
     7801 cggtcgccta cgagctcggt tatgaccacg ccctctttaa caactttttg ctccacctgg
     7861 cggcctatta caaggacatc accgatcagg cctactcggt gcgctatatc agctatgacg
     7921 gcaaggtcaa ctattacaag aacaccaaca actcctatga ggatattcgc ggcttcgaaa
     7981 tcgacctctc gcgcaccatg ggccgctggc tggtcgggga tatcaactat gaataccgcg
     8041 tggagacctc gggctatttc ggccgcgcct accagtatga ggatccggcc gcacagcgcg
     8101 agtacaacaa gagcaatccg gtgcagagca aaccgcgtcc cacgccgcgt ctgaaggcct
     8161 acctggattt cagcaccccg caggatttcg gggccttgct gggcgattgg catgccacgg
     8221 ctacggcgag ctggaccgcc ggctggtggg gcacctggaa tccccaggat atcgccggta
     8281 tcctttacaa cacccagtac aaggactatt acaacctcaa cctgaagttt tcaaagactt
     8341 tctacgtggg cccgatggag ttgaagggtt ttgtcgatgt ctataatgcc ctcaatttca
     8401 agcgcgtttc cggttacggc catgtcgata cctatgacta taactactac atgaaatccc
     8461 tgcacctgcc ggcgtccatc acccgcgacc tcggctacaa cgccatcccg gggagcgatc
     8521 gccctggcga ttaccgcgag gatgatgtcg cgttcgtgcc catggagtat gtgaccgacg
     8581 taaacgcgat cgctgcgccg agcacgcgcg cggtctatta tgagggaccc tccgagcgtt
     8641 atatgcagta cgtcgacggc cagtggcaga aggtgccgga agccgagctg cagcagatgc
     8701 tggatgacaa ggcttatatc gatatgccga atcagagttt cctgaatttc ctcaatccgc
     8761 gccagatctt tttcggcatc accctgaact ataattttta actttctcat cccctaaccg
     8821 agaaggaaca gatatgagag taaaaaattc gctgtgctgg ctcgccctaa ttgcagccat
     8881 tcttctggtc accattccgg tccaggcgga taataccaag tggctggcca cagggatgct
     8941 gcacgactgg ttctccagcg gcggttatga ggtcgaagtc ggccgccgtc atctggtggc
     9001 cgaccagcag gacggcctgc agtggcccgc cctttttgaa tatcaggact gcaaggcagc
     9061 aaaggccctc tggatcggca cgaccaacta taacgatccg gtcgccggca aggt
//
LOCUS       DAOPWU010000124         9063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7681_length_9063_cov_5.422957, whole genome shotgun sequence.
ACCESSION   DAOPWU010000124 DAOPWU010000000
VERSION     DAOPWU010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9063
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7681_length_9063_cov_5.422957"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2206
                     /locus_tag="PKM23_07860"
     CDS             <1..2206
                     /locus_tag="PKM23_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91428.1"
                     /translation="PALPDPFAWSDGRGRVAHKLDWRWRRAEISAMLQHYQLGEKPPA
                     PDSLTASLSGNRLTVTVVEGGKSLTLTAVITLPACGSAPYPAVIGVGGGTGSLPAELF
                     TSRCVATISYNFGEVAPWTQSGRGQGGFYTLYPDTKVGYFTAWAWGISRLIDGLQRLP
                     ETQIDTRRLAVTGCSFAGKIALYSGALDERIALTLAQEPGGGGDAAWRVTETLSGSRE
                     TLRNAQSYGWYHQDLTQFNNAVTRLPIDQHEVMALIAPRAFLLLGNPDMEWLAEESGY
                     VSCMAAREVWQALGVPERFGFSKVGGHNHCNLPDTQRPEVGAFIDRFLLGVEETPTDY
                     AIHPGYTTSLANWIKWNTPVLGSGASFFGRALLLEPPDQQTELGAEVTCRWSRVEGAA
                     RYLFEMSDDPLFAAVAVRDSTADTLKILTGLPRGMRCYWRVRVQDSAGSVGPWSPQWS
                     FSTYIPMPGETRLVAATPLPNRADYIQLKWSRAENATEYWLQLSAQASFNRLISSAVT
                     SDTSRNLNGTTEGVHYYWRVQPRNVAGTAAWSATGDFTIIIAPSDLAVQVNSAYQAVL
                     TWRDRSKVEGGYVVERKAGAQGEFAMLVTLASGATTWTDSTVQKGESYTWRVRAAKDS
                     LTSLPGNEVSLTLTSVRGEGALPLEYSLSQNWPNPFNPSTRIAFSLPRAEWARIALYD
                     LLGREVRILLDQEMAAGHHQVGFEAGQLAGGVYICRMHSGDFIAARKMILMK"
     gene            complement(2335..3765)
                     /locus_tag="PKM23_07865"
     CDS             complement(2335..3765)
                     /locus_tag="PKM23_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNY91429.1"
                     /translation="MPLTAAWQADLNSNYGNQAQPLMLSSKGEVIWSDRPFKIDFAPG
                     KLKITGEVEYSKGHGTLKGAYLHASRNYYPPSGKMPDSLLFSHPQYNTWIELMYDQNQ
                     RDVLAYAGHIIANGFPPGVLMIDDNWQEDYGKWNFHPGRFPEPKEMVDQLHAMGFKVM
                     VWVCPFVSPDCDVYRELESKKYLVTNQNGEPAIVRWWNGASALLDLTHPDAFRWFRSQ
                     LDRLMNEYGVDGFKLDAGDFEYYQDVHSWKADARPEEHSELFGKIGLAYPLNEYRAMW
                     KMGGQPLAERLRDKQHGFRDLQLLIPNMLTAGLMGYYFSCPDMIGGGEFTSFLDDAIL
                     DQESIVRSAQCHALMPMMQFSVAPWRVLGESHLAAVKKAVAIRERFSGYILELAARAA
                     KTGEPIMRPLEYAYPDQGCATITDQFLLGERLLVAPILTRGAVTREVVIPTGRWKSFD
                     GRIVRGPKRITVQAGLDVLPYFERLP"
     gene            4445..5725
                     /locus_tag="PKM23_07870"
     CDS             4445..5725
                     /locus_tag="PKM23_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNY91430.1"
                     /translation="MAHLASRSAYQDLVERLNRFPQGAPPSELLYKILQLLFSEREAQ
                     LVALLPIKPFRAAAAARVWKLTLAESRAVLDQLADKGILLDVDKNGEPVYTLPPPMAG
                     FFEFSMMRVRTDIDQKVLAELFYQYLNVEEDFIKALFTDGQTQLGRTFVHEPALTPEI
                     SLHVLEYERASEVIRSARFRAVGVCYCRHKMEHLGKACSAPLDICMTFNGPAESLIKH
                     KVARAIDVPEGLDLLQQAYEHNLVQFGENVRESVGFICNCCGCCCEAMLAAKRFGAMH
                     PVHTTNYIPEIEESRCNGCGKCVEVCPVEAMALVSAGDPHHPKKKKAHLLEDICLGCG
                     VCVRSCPSASLHLKPRASRVITPLNSTHRAVVMAVERGKLQNLIFDQGALTSHRAMAA
                     VLGVLLKVPPIKQAMASRQIRSRYFEWLVSKRAS"
     gene            6327..7601
                     /locus_tag="PKM23_07875"
     CDS             6327..7601
                     /locus_tag="PKM23_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91431.1"
                     /translation="MLRSEKRWRQRDRISFFIHQDQAMKLKPNSPIGRLLQKARQSYY
                     RLPGFVQLTIRTASNLRWAFSALRVELWLITRKGDAQQEGLKIYYSGSEKTRQLISRL
                     AFDDDCEASFISKTWLWRASRTLSRCEDEDLLSVIEMPFFMSRVFKKEQRFFIPNWIH
                     ADVDISIEMNRLLKNSSLQSDLRRIRKNNLTFEVVNDVQHLQEFYSNMYVPLITQSHG
                     SEAIIDPYLTMEREFLNGADLLLVAKDGKHLGGMLIIYPSSTAYLWRIGILNGDVQFI
                     QEGAISALYYYSILFLREKGYRRVHFGMTRAFLKDGVLQYKRKWGLTLVGKPNMGILI
                     GMNPRGQQARQFLLDNPFIFMQNKKFYSGLFTEKPLLDEDDFQQFNKEYYYDGIETMV
                     IHRFDVENPQVGCSIPEELVARVRLVSIKCGD"
     gene            complement(7791..8837)
                     /locus_tag="PKM23_07880"
     CDS             complement(7791..8837)
                     /locus_tag="PKM23_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91432.1"
                     /translation="MMRELEKSCRYLLHTILGLQRGDKLLLYTDESDGQGTAKAIQEI
                     SLADEISADLVPLQEFRDLPEKEAALLDKIAHGNYQALCELSSQYFYPSPVWRKAVRQ
                     GCRVLALGAMDKEAFVRCVGKVGHEKIYEFGLRLSDLLRKARWVEIFSDAGAKISCQL
                     SRSHGLISRIGRKLGLQANSHVWTPSGMFTPSIHATFMSGQVAFIGMPHSIQGTAVID
                     GSLWPPDTIGKLDHPLLLRLEKGSMISVEGSSDKAIILNQWLQGRDKQIKHFCIGFNP
                     GAHLSKNLVETERAFGHLGVGVGTFPFHTDGIIKNPTIVLDGTVLLQNQTFQDAGLMA
                     LSRELVVDIAGKDG"
BASE COUNT         1934 a         2622 c         2533 g         1974 t
ORIGIN      
        1 tcccgctctt ccagatccct tcgcctggtc cgacggccgc ggccgtgtcg cccacaagtt
       61 ggactggcgt tggcggcgtg ctgagatcag cgccatgctg cagcactatc agctcgggga
      121 gaaaccgccc gcgccggaca gcctgacagc ctccctctcc ggcaaccgcc tcacggtgac
      181 ggtggtggag ggcggcaaat ccctgaccct gacggcggtg atcaccctcc ccgcctgtgg
      241 cagtgccccc tacccggccg tcatcggcgt cggcggcggc accggcagcc tgcccgccga
      301 gctcttcacc agccgctgcg tcgccaccat tagttacaac tttggcgagg tggcgccctg
      361 gacccagagc ggccgcggcc aggggggatt ctacaccctc tatccggata ccaaagtggg
      421 ctacttcacg gcctgggcct gggggatcag ccgcctcatc gacggactgc agcggctgcc
      481 ggagacgcag atcgacaccc gccgcctcgc cgtaaccggc tgctccttcg ccggcaagat
      541 cgccctctac tccggagcgc tcgatgagcg catcgccctc accctcgccc aggaaccggg
      601 cggcggcggc gatgccgcct ggcgcgtcac tgaaaccctg agcggcagcc gcgagaccct
      661 gcgcaacgcc cagagctacg gctggtatca tcaggatctg acccagttca acaacgccgt
      721 aacccggctg cccatcgacc agcacgaggt catggccctg atcgcccccc gcgcttttct
      781 gctgctcggc aatccagaca tggagtggct ggccgaagag tccggctacg tcagctgcat
      841 ggccgcccgc gaggtatggc aggccctcgg cgtgccggag cgcttcggct tctccaaggt
      901 gggcgggcac aaccactgca atctcccgga cacccagcgc cccgaggtgg gtgccttcat
      961 cgaccgcttc ctgctgggtg tggaggagac cccgaccgac tatgccatcc atcccggcta
     1021 taccaccagc ctcgccaact ggatcaaatg gaatacgccg gtgctcggca gcggcgcctc
     1081 ctttttcggc cgtgctctgc tcctcgaacc gcccgatcag cagaccgagc tcggcgccga
     1141 ggtcacctgc cggtggagcc gggtggaagg ggcggcgcgt tacctctttg agatgtccga
     1201 cgaccccctc ttcgccgcag ttgccgtccg cgattccacg gcggataccc tgaaaatcct
     1261 caccgggctg cccaggggga tgcgctgcta ctggcgggtg cgggtgcagg acagcgccgg
     1321 atccgtcggg ccctggagcc cccagtggag cttttccacc tacattccca tgccggggga
     1381 gacccggctg gtcgccgcca cgcccctgcc caaccgcgcc gactatattc aactgaaatg
     1441 gagcagggcg gaaaacgcca ccgagtattg gctgcagctc tccgcccagg cctccttcaa
     1501 ccgcctgatc tcctccgcgg tgacctcgga taccagccgc aatctcaacg gcacaaccga
     1561 gggggtacac tactactggc gggtgcagcc gcgcaatgtt gccggcacgg ccgcgtggag
     1621 cgcgaccggc gactttacca tcatcatcgc cccgagcgac ctcgccgtcc aggttaacag
     1681 tgcctatcag gctgtgctca cctggagaga ccgttcgaag gtggaagggg gctatgtggt
     1741 cgagcggaag gcgggtgctc agggtgagtt tgcgatgctc gttacgctcg cgagcggtgc
     1801 gaccacctgg accgacagca ccgtgcaaaa gggggagagc tacacctggc gggtcagggc
     1861 ggccaaagat tcgctcacct cactgcccgg caacgaggtc tcgctgaccc ttacctctgt
     1921 gcgcggtgag ggcgcgctgc ccctggaata ctccctgagc cagaactggc ccaatccctt
     1981 caatccctcg acgcggatcg ccttttccct gcccagggcg gaatgggcca ggatcgctct
     2041 ctacgacctg ctcggccgcg aggttcggat tttgctcgat caggagatgg cggcgggcca
     2101 tcatcaggtc gggttcgaag ccgggcaact cgccggcggg gtctacatct gccggatgca
     2161 ctccggcgat ttcattgccg cccggaagat gattctgatg aagtgacctc aatccgctgt
     2221 aaagaaaaat gccctccaca ccggagggca ttttcatttt ggggatgctg ctctgttcac
     2281 tcccgagccg gctcagcggc gcctggccgc tgactgccgc ttgcgtcggg cgcgctatgg
     2341 cagccgttca aaatagggca gcacatccag cccggcctgg acggtgattc gcttaggtcc
     2401 ccggacgatc cggccgtcga agcttttcca gcgccccgtt gggatgacca cctctcgcgt
     2461 cactgccccc ctggtcagta tcggcgccac cagcagccgc tcaccaagca aaaattggtc
     2521 ggtgatcgtc gcacagccct gatccggata ggcatactcg agtggtctca tgatcggctc
     2581 gccggttttg gccgcccgtg cggccagctc cagaatatac ccgctgaatc gttcccggat
     2641 cgccaccgct tttttgacag cggcgaggtg gctctcaccc agaacccgcc agggggccac
     2701 cgaaaactgc atcatcggca tcagggcatg acactgcgcg gagcggacga tcgattcctg
     2761 atcaaggatt gcatcatcca gaaaggaggt gaattcgccg ccgccgatca tgtctggaca
     2821 ggaaaaatag tagcccatca gtccggcggt cagcatgttc gggatcagca gctgcagatc
     2881 gcgaaagcca tgctgcttgt cccgcagccg ctccgccagc ggctggccgc ccattttcca
     2941 catcgcccgg tactcattca ggggataagc caggccgatt ttgccgaaca gctccgagtg
     3001 ctcctccggc cgggcatccg ccttccagga atgcacatcc tggtaatact cgaaatcccc
     3061 ggcgtccagt ttaaagccgt caaccccgta ctcgttcatc agacggtcca gttgtgagcg
     3121 gaaccagcga aaggcatcgg gatgggtcaa gtcgagcagg gcactggcgc cgttccacca
     3181 gcgcacaatt gccggctctc cgttctggtt ggtgacgaga tacttcttgc tctccagctc
     3241 ccggtagaca tcgcagtccg gactgacaaa ggggcacacc cagaccatca ccttgaagcc
     3301 catcgcgtgc agttgatcga ccatctcttt gggttcgggg aagcgtccgg ggtgaaaatt
     3361 ccatttgccg taatcctcct gccagttgtc atcgatcatc agcacgccgg gtggaaaccc
     3421 gttggcgatg atatggccgg cgtaagccag gacgtcccgc tggttttgat catacatcag
     3481 ctcgatccag gtattgtact ggggatggct gaacagcagc gagtcgggca ttttgccgga
     3541 aggcggataa tagttccggc tggcatggag gtaggccccc ttgagcgtgc catgcccttt
     3601 ggaatactcg acctcacccg ttatcttcag cttgcccggg gcaaaatcga tcttgaaggg
     3661 gcggtcggac cagatcacct cgcccttgct ggagagcatc agcggctgtg cctgattgcc
     3721 atagttactg ttcagatctg cctgccaggc tgcggtcagc ggcatcctgt tgccttgatt
     3781 gatcaccccc gcccaccagt aggcgtctgc accgacttta atgctgagag tgtcctgctg
     3841 cccgtacgct gggccaaaca gccataaggc ggagagcagg attagtagcg gagatgatga
     3901 aatggcttta ttgttcagat tcatggttat cctttcagag tcggcaatct gcctggcgtt
     3961 gactcggtag aataaaacac ttccgttaca aagaataaaa aggctggtga tatttcgtga
     4021 agggtcaatc tacttaattt gtagaataaa ataactcaat atattgaaga aaagcaagac
     4081 ctgtttaata atgcgcgggt cccttgtccg gcttgatgcc gtgcatatca cccgggggaa
     4141 tagggggaac agatcgatgg cggcaggatg ggcatcgctt gtaaatggca agtcctgcaa
     4201 atcgtccttc ctttaattcc gcaggcaagg ttgatcctgt cggaatttcg cgtgattcga
     4261 tgtttcttgt tacatttaac ttgattttcc tttgctgttt ggctaatttt gcgcaagtcg
     4321 gcagcggcaa gaccagtctt tgatggtcca ctggctcaag gaggggcgct gcatggaact
     4381 cccctcgaaa agaaaggtga atccgccgat cccggggaca aatagcagag tataggagca
     4441 ctttatggct catctcgcct cccgttccgc ctaccaagat cttgttgaac gtctgaaccg
     4501 gttcccgcag ggggctcctc cctcggagct tctctacaaa attctgcagc tgctcttctc
     4561 cgaaagagag gcgcagctgg tcgcccttct ccccatcaaa cccttccggg ctgccgctgc
     4621 ggccagggtg tggaagctta cgctcgccga gagcagggcc gtccttgatc agctggctga
     4681 caaggggatt ctgctcgatg tcgacaaaaa cggtgagccg gtatacaccc ttcccccgcc
     4741 gatggcggga ttctttgaat tttcgatgat gcgggtgcga acggacatcg atcagaaggt
     4801 tcttgcggaa cttttctatc aatatctcaa tgttgaagag gatttcatca aggcgctctt
     4861 tacagatggg cagacgcagc tgggacgaac ctttgtccac gagccggcac tgacaccgga
     4921 aatctccctg cacgtgctgg agtacgagcg ggcgagtgag gtcatccgca gcgcccgatt
     4981 ccgcgctgtg ggcgtctgtt actgccgcca caagatggag catctgggca aggcctgcag
     5041 cgccccgctc gacatctgta tgaccttcaa cggcccggcc gagtccctga tcaagcataa
     5101 ggtcgcccgg gcgatcgacg tcccggaggg tctcgatctg ctgcagcagg cctatgagca
     5161 caatctggtt cagttcggag agaatgtccg ggaatcggta gggttcatct gtaactgctg
     5221 cgggtgctgc tgcgaggcga tgctggcggc gaaacggttc ggggcgatgc acccggtaca
     5281 tacgaccaac tacatcccgg agatcgagga gagcaggtgc aatggctgcg gcaagtgcgt
     5341 ggaggtttgt ccggttgagg cgatggccct ggtctcggcc ggtgatccgc accatccgaa
     5401 gaaaaagaag gctcatctcc ttgaagacat ttgtctcggc tgcggcgtgt gtgtccggtc
     5461 ctgtccgtca gcaagccttc acctcaagcc cagggcctcc cgcgtgatca cgccgctcaa
     5521 ttccacccac cgcgcggtgg tcatggccgt cgagagggga aagctgcaaa acctgatctt
     5581 cgaccagggt gcgctcacca gccatcgggc gatggctgca gtactgggcg tgctgctgaa
     5641 agtgcctccg atcaagcagg ccatggccag ccggcagatc aggtcgagat actttgaatg
     5701 gctggtctcc aaacgagcga gctgaacgcc tcactgatgg aggagttgga cggacttgct
     5761 ctgtccagag atcattttcg aataatcccg tttcacatgc gaagtggtgc aatcggtgtg
     5821 ggtatgcccg cacctgggct ccggctttga cagcacagtg cggattgagg ttggcgatga
     5881 ggaggggatg cagcggcgac ttgtttcctg ttgttggcca atccatacaa atcctttgaa
     5941 gcccggttta tcagcagagc attgttgatc atggagcgat acagactggt caggttgatc
     6001 ccgcagcggt gcagccaatt tgcatgtttt caggaatttt ccttgttttt cagttctgca
     6061 ttggttattt ttcggcaagt tgggggcgat gcggatgccg gctttggcat gacgctctga
     6121 aaatagggaa atgattgggt atcaaaccct tgacaccgga tgatttcttc gatttttcta
     6181 cctcccgcca aactcaatat tcatatccat tccaagcctt gcgcaaactg gttctctagg
     6241 aatagaagat tggctgcatc cggcgcaaag ttcagccgca gggactctga ttgacaacaa
     6301 ccacccctgg gggatcacca gtccgggtgc tcagatctga aaaaagatgg cgtcaacgag
     6361 accggatttc attttttatt catcaagacc aagccatgaa attgaaaccg aacagcccca
     6421 tcgggcgact cttgcaaaaa gccagacaga gctattatcg tttgccaggg tttgtacaac
     6481 tgaccatcag aacggcatca aaccttcgtt gggctttttc tgcgctgcga gtggagctct
     6541 ggctcatcac ccggaaggga gatgcacagc aagaggggtt aaaaatttat tatagcggca
     6601 gtgaaaagac ccgacaactc atttcgcgat tagcctttga cgacgattgc gaggccagtt
     6661 ttatcagcaa aacgtggttg tggcgggcca gccgaactct ttctcgctgt gaggatgaag
     6721 atctgctctc tgtaattgag atgccgttct ttatgtcgcg agttttcaaa aaagagcagc
     6781 gcttttttat acccaactgg atccatgccg atgtcgatat ctcgatagaa atgaaccggt
     6841 tgttgaaaaa ttccagcctg caatcggatc ttcgcaggat tcgaaaaaac aacctgacct
     6901 ttgaggttgt caatgacgtg cagcacctgc aggaatttta ttccaacatg tacgtcccgc
     6961 ttatcaccca aagccatgga tcagaggcga tcattgatcc gtacctgacc atggagagag
     7021 aatttttaaa cggtgcggat cttttgctgg tagctaaaga cggcaaacat ctgggtggca
     7081 tgttgatcat ctatccttca tcgactgcct atctatggcg tatcggcatc ttgaatggtg
     7141 atgtgcagtt tattcaggaa ggcgcgatca gcgcgcttta ttactattca attctctttt
     7201 tacgggaaaa aggatatcgc cgggtgcact ttggcatgac acgcgctttt ctcaaggatg
     7261 gggtgctgca gtacaagagg aaatggggat tgacattggt tggcaaaccc aacatgggaa
     7321 ttctaatcgg tatgaacccg cgaggccaac aggcaagaca gtttctgctg gacaatcctt
     7381 tcatttttat gcaaaacaag aaattttaca gcggcctttt cactgaaaag cccctgttgg
     7441 atgaggatga tttccagcag ttcaataaag agtattacta tgacggcatc gaaacaatgg
     7501 tgatccatcg ctttgacgtt gaaaatcctc aagtgggctg ttccatcccg gaagagctgg
     7561 ttgcccgagt caggctcgtc tcgataaaat gcggggactg aggataaatc cctggctgtg
     7621 ctccgccttt ccatactacc cccgaggaca tcccggacca ggaccggcga atcttttatg
     7681 ccatccaaat catcgggctt gcttctgatt caatggctct gtcggctgct ttagcttcac
     7741 aatgcagttt tatcgtctca gatgcaatag acccaaacgg gccgattcag ctagccatct
     7801 ttcccggcaa tatccacaac cagttccctg ctcagcgcca tcaacccggc atcctgaaac
     7861 gtttgattct gcaacaggac ggttccatcg agaactattg tgggattctt tatgatgccg
     7921 tcagtgtgaa atggaaaagt tccgaccccc accccaagat ggccaaaagc gcgctcggtt
     7981 tcgacaaggt tttttgacaa gtgagcgccg ggattaaagc caatgcagaa atgcttgatc
     8041 tgcttatcgc ggccctgcaa ccattgattc aaaattatgg ccttgtcact ggatccttcg
     8101 acggagatca tcgagccctt ttcaaggcga agaagcaagg ggtgatcaag cttgccaatc
     8161 gtatccggcg gccaaagaga gccatcgata accgctgtcc cctgaatcga atggggcatg
     8221 ccaatgaagg ccacctgccc actcataaaa gtcgcatgaa tgctcggcgt gaacataccg
     8281 gatggcgtcc aaacgtgcga gttcgcctgg aggccgagtt tcctgccgat tcgactgatc
     8341 aagccatgtg aacgcgaaag ttggcagctg attttggccc ccgcatcgga aaaaatttcc
     8401 acccaacgcg ctttacgcag caggtcggac agccgcaagc caaactcata aatcttttca
     8461 tggcccacct tgccgacaca acggacaaag gcctctttgt ccatcgcccc caaggctaaa
     8521 acccggcagc cctgtcgaac agcttttctc cagaccggtg aaggataaaa atactgggaa
     8581 gaaagttcgc agagggcttg gtaattccca tgtgcaattt tgtccagcaa ggcggcttct
     8641 ttttccggga ggtcgcggaa ctcttgcaag gggaccaggt ctgcgcttat ttcatcagcc
     8701 agggagatct cctggatggc tttggctgta ccctgcccat cgctctcatc ggtatacagt
     8761 aacaacttgt cgccacgctg cagacccaga atcgtgtgca agagatagcg acagcttttt
     8821 tccaattcgc gcatcatccg tttcctcttt atttggttcc catcatcaga cctggcagca
     8881 tggggcgagg tccagcctgc gaccgggctg ctcttgatgt gaaggagacc gggtgtcagc
     8941 gtcgccccgg gacaagtgag taagatatcc tcttcagttt tgaagagagg agaccgaacg
     9001 acgattacat gctcatgcga gaaaaaaagt ggaatctgaa agccgtactg cgtacactac
     9061 tta
//
LOCUS       DAOPWU010000125         9034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7714_length_9034_cov_4.857334, whole genome shotgun sequence.
ACCESSION   DAOPWU010000125 DAOPWU010000000
VERSION     DAOPWU010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7714_length_9034_cov_4.857334"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PKM23_07885"
     CDS             complement(<1..867)
                     /locus_tag="PKM23_07885"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNY91433.1"
                     /translation="MFVYGSLNDDHHFRLLTGMSLEGIPARLANFRRIHPKNAFAFAV
                     PWSGGHIEGRLLSGVTPALLAKLDEYESAGSLYERLIVEVEAGGRKQQAWIYIADPEA
                     IRPWLERGFAERDRIEAFVEQSVHRYLETKADRCPLGGRRALALAVTGELLSEEIQGL
                     LSQALEDEQLAPWLIKHEIEKADLPGLEWLEGDRKARPFAGAYMSLALRLMIFNQIET
                     RFRHNFRGLTRTGEELYERSLSALMALKLLVDQGPALKELIARQGVDTFRPDFTYPDY
                     AVAALFIAEELWQ"
     gene            complement(1033..2397)
                     /locus_tag="PKM23_07890"
     CDS             complement(1033..2397)
                     /locus_tag="PKM23_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNY91434.1"
                     /translation="MSTLKAPWQTMAKSPRHPLIDVEEPNLMRDVFPYDQIPRTVFDG
                     ITVDARPAAEIWMTDTTFRDGQQARTPFTVEQASTLFDLIHRLSGPNGVIRQSEFFIY
                     SDRDREILEEVRSKGYRFPEVTAWIRANPADFELVRRAGLKETGLLTSISDYHIFLKF
                     KKSRRQVLDDFMKVVRAAAEAGLEAIRCHYEDVTRADFWGAVVPFTEELMDFSAHSGL
                     RIKIRLCDTMGYGLPYADAALPRSVPKLIYYLQREFGIPSRDLEWHGHNDFHKVHING
                     VAAWLSGASSVNGTILGFGERTGNSPLEALAVEYASLTGSTNGMDLAAITGIADYMRS
                     IGTAIAPNYPFVGSNFNVTMAGIHADGVIKNEEIYNIFDTGKILNRPLGVHITDRSGI
                     AGVGFWVSQQLQQQGKPPVDKRDPRVAKIFEWIEAQYADGRVTAISQEEMQQQFQLHF
                     NQTA"
     gene            complement(2552..3223)
                     /locus_tag="PKM23_07895"
     CDS             complement(2552..3223)
                     /locus_tag="PKM23_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY91435.1"
                     /translation="MSDTSSGLMDDMELLEADQLDDLTRDLEKHIVEDPTDLRSLLML
                     GNGYYLRGKIGAAIQTFQKAVSVNPRLPYAYYYLGVSYYRAMQLDEAIAALEQSIASS
                     PTMIMAHYWLGMAYYHKGLYFKARTAFETLLESNRESVIGHYHAALACMADQAYECAL
                     EHLQRLAMAGHRDPHVMLYLGNVYFRLNRTREAIAAYQEGLAIHPDNRLLRDALEHLT
                     EVQEP"
     gene            3371..3946
                     /locus_tag="PKM23_07900"
     CDS             3371..3946
                     /locus_tag="PKM23_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNY91436.1"
                     /translation="MKKCWYGLLFIVIVLAGGAVSAQERTSTPPVAPLPGAAAPDSAL
                     EQEEEWDAAGLGSFLSDFTTSRGEAISGGELLSRIKSKAEVDSSRMVEGFRVQLMATR
                     DESEARRARDDARTYFPENSYLLFDNPYYKLRLGDCLTRTSADSLQQRAVNKGFNGAW
                     IVRSLVHEFTPQLNIFYLPPPDSGSADWENP"
     gene            4109..4858
                     /locus_tag="PKM23_07905"
     CDS             4109..4858
                     /locus_tag="PKM23_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011190149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNY91437.1"
                     /translation="MSGHSKWATIKRKKEKTDAARGRAFTRLIKEITIAARHGGGDET
                     SNPRLRTAVLAAKAANMPAANIDRAVKRGTGELPGVNYEEITYEGYGPGGVAVYMEVL
                     TDNKNRTVAEIRHLLSKYNGALGESGSVAWMFSKKGVIMVPSKNTTEDDLMMATLDAG
                     ADDIAAEEEYFRVTTEVNKLEAVKKALEAAKIGYESAEITMEPANQVKVEGKAAESVI
                     KLMDALEEHEDIQNVYANFDIDEKTLEEMED"
     gene            4865..5362
                     /gene="ruvC"
                     /locus_tag="PKM23_07910"
     CDS             4865..5362
                     /gene="ruvC"
                     /locus_tag="PKM23_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNY91438.1"
                     /translation="MVVMGIDPGSQITGYGIVAISAGEAPRHVACGAIRTRSCDDLYA
                     RLKLIYDEVAQLIALHRPDQVALEDIFYARNARSSLLLGQARAAAVLAALNGGVPVSA
                     YSPSEVKMAVTGRGSAAKEQVRYMVMQLLGITLSGESLDVSDALAIALCHALRGQSSM
                     RRTRS"
     gene            5380..5976
                     /gene="ruvA"
                     /locus_tag="PKM23_07915"
     CDS             5380..5976
                     /gene="ruvA"
                     /locus_tag="PKM23_07915"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNY91439.1"
                     /translation="MISFVRAILTDKQPTHVVVDREGLGLEILIPLSTYAALPGVGES
                     VLLHTYLHVREDALILIGFATPAEKELFQLLLSVSGIGVRSALAILSGCRAEELYSWI
                     ARGDEAALTRIPGLGKKTAQRMILDLKEKAAQQLQKGGITAAPAPALASGLSEQALQA
                     LTGLGFSRLEAQKALEKAAARLGEGAELEELLRSALQG"
     gene            5988..7019
                     /gene="ruvB"
                     /locus_tag="PKM23_07920"
     CDS             5988..7019
                     /gene="ruvB"
                     /locus_tag="PKM23_07920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNY91440.1"
                     /translation="MVNQSDRPTYPEALNGEREFDATLRPERFGEFIGQDKLKENLHI
                     FIQAARARQEALDHVLFYGPPGLGKTTLAHIIAREMGSQIRLTSGPTLERAGDLAGLL
                     TNLGEHDVLFIDEIHRLNRVVEEYLYPAMEDFKLDIILDKGANARSIQLKLPHFTLVG
                     ATTRAGLLTSPLRARFGVSFRLDFYEPERLCEIVLRSARILGKKIDPAAALEIARRSR
                     GTPRVANRLLRRIRDFAQILGEEEITLERAASALQRLDVDESGLDEMDKRILLTVIDK
                     FSGGPVGLNTLAVAIGEEPDTIEELYEPYLIQSGFLNRTPRGRTATDAAYTHFHRRHG
                     GARQQQLFD"
     gene            7124..8152
                     /gene="queA"
                     /locus_tag="PKM23_07925"
     CDS             7124..8152
                     /gene="queA"
                     /locus_tag="PKM23_07925"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNY91441.1"
                     /translation="MKLSDFKYNLPDKLIAQYPSDERDQCRLMILNRESGTMEEGIFA
                     DVIDYMEKGDSLVINQTKVFPARLEGSKDKTDARVEVFLLRELEQGLWEVLVKPARKV
                     RVGNRLTIGNELACDVIDNTVSGGRVVRFNYSGDFYEIVDRIGKSPLPPYIRREPEPS
                     DKLRYQTVYARERGAVAAPTAGLHFTEPLLKKIKAKGIKIVPLTLHLGLGSFRAVVVE
                     DLSRHKMDSEFFEISEEAAEAINETMRSKHKVVAVGTSVVRALEANVTSEGWAKAGHG
                     WTDKFIYPPYEFRIVDRMITNFHLPQSTLLMLVSAFAGRDLVFKAYRKAIRDKFMFFS
                     YGDSMLIL"
     gene            8171..8860
                     /gene="ispD"
                     /locus_tag="PKM23_07930"
     CDS             8171..8860
                     /gene="ispD"
                     /locus_tag="PKM23_07930"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNY91442.1"
                     /translation="MEKRTKRVALIVAAGQGKRFGGAVSKQFVEVGGRPLLWYTLTAF
                     ERCPDIDEILVILPAGELGERTAEIAAWGLTKFCAAVAGGKERHDSVFNGLQALSSQA
                     GLVAIHDGARPLITPELISACLRAAEECGAAFAGIAPKDTIKEVEGGLVTATLDRRRL
                     VLVQTPQVFRVDLVRHAYDSAFERGAFSTDDAALVEALGHPVRVVPGDYRNLKVTSPE
                     DLLFVKACLEI"
     gene            8879..>9034
                     /locus_tag="PKM23_07935"
     CDS             8879..>9034
                     /locus_tag="PKM23_07935"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNY91443.1"
                     /translation="MRIGHGFDVHRLVSGRKLILGGVEIPFELGLLGHSDADVLCHAV
                     SDALLGAL"
BASE COUNT         1679 a         2853 c         2858 g         1644 t
ORIGIN      
        1 ctgccaaagc tcctcggcga tgaagagagc ggcgacggcg taatcgggat aggtgaaatc
       61 ggggcggaag gtgtcgaccc cctgccgggc aatcaactcc ttcagggccg ggccctggtc
      121 gacgaggagc ttgagggcca tgagggcgga gaggctgcgt tcgtagagct cttcaccggt
      181 gcgggtcagt ccgcggaaat tgtgcctgaa cctggtttcg atctggttga aaatcatcaa
      241 cctgagcgcc aggctcatgt aggccccggc gaaggggcgg gccttgcggt cgccctcgag
      301 ccattccagg ccggggaggt cggccttttc gatttcgtgt ttgatcagcc agggggcgag
      361 ctgttcatcc tcgagggcct ggctgagcag cccctgaatc tcctcggaga gcagctcacc
      421 ggtgacggcc agggcgaggg ccctgcggcc gccgaggggg cagcggtccg ctttggtctc
      481 gagatagcgg tgtacgctct gctcgacaaa ggcctcgatg cggtcgcgct cggcgaagcc
      541 gcgttccagc caggggcgga tggcctctgg atcggcgatg tagatccagg cctgctgctt
      601 gcggccgccg gcctcgacct cgacgatgag gcgctcgtag agtgagccgg ctgattcgta
      661 ttcatcgagt ttggcgagca gggcgggggt gaccccggag aggagccgtc cctcgatgtg
      721 gccgccgctc caggggacag caaaagcgaa tgcgtttttg gggtggatgc gccggaagtt
      781 ggcgagcctg gccgggatcc cctccaggct cattccggtt aacaggcgga agtgatggtc
      841 gtcattgagg gagccgtaaa caaacaatgc agcgatctcc ccctgagtcc ggtccgggga
      901 cccaagggga gccgtttttg ccgatgttgc gggtgccgga gaattccggg cattcacgac
      961 catgatgaag gccgcgcggc gttcggcgcc gcgcgctcat gcgggtgatg gagtggcctg
     1021 acctccgtgc gatcaggcgg tctgattaaa atggagctgg aactgctgct gcatctcctc
     1081 ctgggagatg gccgtgaccc gtccgtcggc gtactgcgcc tcgatccact cgaagatttt
     1141 cgccacacgc gggtcgcgct tgtccaccgg cggcttgccc tgctgctgaa gctgctggct
     1201 gacccagaag ccgaccccgg cgatgccgga gcggtcggtg atgtggacgc cgagcgggcg
     1261 gttgaggatc ttgccggtgt cgaaaatgtt ataaatctct tcattcttga tcaccccgtc
     1321 ggcgtggatc ccggccatgg tgacattgaa attggagccg acgaaggggt agttgggggc
     1381 gatggcggtg ccgatgctgc gcatatagtc ggcgatcccg gtgatggcgg cgagatccat
     1441 gccgttggtc gagccggtga gcgaggcgta ttcgaccgcc agggcctcga gcggggagtt
     1501 gccggtgcgt tcgccgaagc cgaggatggt gccgttgacc gaggaggccc cggagagcca
     1561 ggcggcgacg ccgttgatat ggaccttgtg aaaatcgttg tggccgtgcc attcaaggtc
     1621 gcgggagggg atgccgaatt cgcgctgcag gtagtagatg agcttgggca ccgagcgcgg
     1681 cagggcggcg tcggcatagg gcaggccgta gcccatagtg tcgcagaggc ggatcttgat
     1741 gcgcaggccg ctgtgggcgg agaaatccat cagctcctcg gtgaagggga cgacggcgcc
     1801 ccaaaagtcg gcgcgggtga catcctcgta gtggcagcgg atggcctcaa ggccggcctc
     1861 ggcagcggcg cgcaccactt tcataaagtc gtcgagcacc tggcggcggc tctttttgaa
     1921 cttgagaaag atgtgatagt cggagatcga ggtcagcagc ccggtctcct tgagtccggc
     1981 ccggcgcacg agctcgaaat cggcgggatt ggcgcggatc caggcggtca cctcggggaa
     2041 gcgatagccc ttgctgcgca cctcctcgag gatttcgcgg tcgcggtcgg aatagatgaa
     2101 gaactcggac tggcggatga cgccgttggg gccggagagg cggtggatga gatcgaaaag
     2161 ggtggaggcc tgctcgacgg tgaagggggt gcgcgcctgc tggccgtcgc ggaaggtggt
     2221 gtcggtcatc cagatctccg cggccggccg ggcgtcgacc gtaatgccgt cgaataccgt
     2281 gcgcgggatc tgatcgtagg gaaagacatc gcgcataaga ttgggctcct cgacatcgat
     2341 caggggatgc cggggggact tggccatcgt ctgccatggg gcttttaagg tcgacataca
     2401 aacctccgtc tgcgctggta aagggttcag tctgctctct ttccggttgg atggaacagg
     2461 ccgtgcaaag gattcgctcc cttgacggaa gattctgtgc gatcaagcat aatacggcag
     2521 tggcggtccg gtgccttggc cgtcctcctc atcagggctc ctggacctcg gtgaggtgtt
     2581 ccagggcgtc gcgcaggaga cggttgtccg gatggattgc gagcccctcc tgataggcgg
     2641 cgatggcctc gcgggtgcga ttgagccgga aatagacgtt gccgaggtag agcatgacgt
     2701 gggggtcgcg atggccggcc atggccagac gctgcagatg ctcgagggcg cattcatagg
     2761 cctggtctgc catgcaggcg agggcggcat gatagtggcc gatgaccgat tcacgattgc
     2821 tctcgagcag ggtctcgaag gcggtgcggg ccttgaaata gagccccttg tggtaatagg
     2881 ccatccccag ccagtagtgg gccatgatca tggtcgggga ggaggcgatc gactgctcga
     2941 gggccgcgat ggcttcatcg agctgcatcg cgcggtagta ggagaccccc aaatagtaat
     3001 aggcataggg gaggcgcggg ttgacgctca ccgccttctg gaaggtctgg atggcggcgc
     3061 cgatctttcc gcgcaggtag tagccgttcc cgagcatgag cagggaacgg agatcggtgg
     3121 ggtcctcgac gatatgtttc tccagatcgc gggtcaggtc atcgagctgg tccgcctcga
     3181 gaagctccat gtcatccatc aatcctgacg atgtatcgct catcttgatc tccttatgat
     3241 gctgccaggg gtagtaggta ttataagctc tttgttcctg atagtcaagg gatattttcg
     3301 ttgattttgt gcgctattat tgataaattc cgcgtacatt gaataacgcg acaacttctg
     3361 cgagaattcc atgaaaaaat gttggtatgg cctgcttttt atcgtcatcg tgctcgccgg
     3421 cggagccgtc tccgcccagg agcgcacctc cacgccgccc gtcgccccgc tgccgggcgc
     3481 ggccgcccct gattccgccc tggagcagga ggaggagtgg gatgccgccg gactgggctc
     3541 cttcctctcg gatttcacca ccagccgcgg cgaagcgatc agcggcggcg agctgctcag
     3601 ccggatcaag agcaaggccg aggttgacag cagccgcatg gtcgagggtt tccgcgtcca
     3661 gctcatggcc acccgggacg agagcgaggc ccggcgcgcc cgcgacgacg cccgcaccta
     3721 tttccctgaa aacagctacc tgctcttcga caacccctac tacaagcttc gtctcggcga
     3781 ctgcctgacg cgcacttccg ccgattccct gcaacagcgc gccgtcaaca agggcttcaa
     3841 cggggcctgg atcgtccgct ccctcgtgca cgaattcacc ccccagttga acatctttta
     3901 tctccccccg ccggattccg gcagtgcaga ctgggaaaac ccctaaaata tttattgatt
     3961 ttaagccatt tttttattat atttaacgcc attttaatcc cgtttgaacg tcatctgctg
     4021 cggatactga tcgcatgaaa taaccgccgg ttcaaggtat tgcccgttat cccatgaggg
     4081 ggtggcgggt ttatttttag gaggcattat gtcaggccac tccaagtggg caaccatcaa
     4141 acgcaagaag gaaaagacgg acgccgcccg cgggcgcgcg ttcacccggt tgatcaagga
     4201 aataaccatc gcggcgcgcc acggcggcgg cgatgagacc tccaatccac gcctccgcac
     4261 cgcggtgctg gccgccaagg ccgccaacat gccggccgcc aacatcgacc gcgccgtcaa
     4321 gcgcggcacc ggcgaactgc cgggggtcaa ctatgaagag atcacctacg aaggctacgg
     4381 acccggcggc gtcgcggtct atatggaggt gctcaccgac aacaagaacc gcaccgtagc
     4441 cgagatccgc cacctccttt ccaagtacaa cggcgccctc ggcgaatcag gcagcgtggc
     4501 ctggatgttc tccaaaaaag gcgttatcat ggtcccgagc aagaatacca ccgaggacga
     4561 tctcatgatg gccaccctcg acgctggcgc cgatgacatc gccgccgagg aggagtattt
     4621 ccgcgtcacc accgaggtga acaagctcga ggcggtcaag aaggccctcg aggcggccaa
     4681 gatcggctac gaatcggccg aaatcaccat ggaaccggcc aaccaggtca aggtcgaggg
     4741 caaggctgcc gaatcggtga tcaagctgat ggatgccctt gaggaacacg aagatatcca
     4801 gaacgtctat gccaactttg acatcgacga aaaaaccctg gaagagatgg aagattaggc
     4861 cgagatggtg gtgatgggga tcgacccggg cagccagatc accggctatg gaatcgtcgc
     4921 catctctgcc ggcgaggcgc cccgtcatgt cgcctgcggg gccatccgca ctcgcagctg
     4981 cgacgacttg tacgcgcggc tcaaactgat ctacgatgag gtcgcgcagc tcatcgccct
     5041 gcaccggccg gaccaggttg cactcgagga tatcttttac gcccgtaacg cccgatcctc
     5101 gctgctgctg gggcaggcgc gcgccgccgc cgtcctggca gccttgaacg ggggggtgcc
     5161 tgtctccgcc tattcaccct cggaagtgaa aatggccgtt accggccgag gcagcgcggc
     5221 caaggaacag gtgcgttata tggttatgca gctgctcggg atcaccctca gcggtgaatc
     5281 cctcgatgtc tccgatgccc tggccatcgc cctctgccac gccctgcgcg ggcagagcag
     5341 catgcgcagg acccgatcct gagaggcggt gctccggtta tgatctcatt cgtccgcgcc
     5401 atattgaccg acaagcagcc gacccatgtc gtcgtcgacc gcgaggggct ggggctcgaa
     5461 atcctcatcc ccctctccac ctatgccgcc ctgcccgggg tgggcgagag cgtcctcctc
     5521 cacacctatc tccacgtccg cgaggatgcc ctcatcctga tcggcttcgc cacccccgcc
     5581 gagaaagagc tcttccagct cctgctctcg gtctcgggca tcggggtgcg ctcggccctg
     5641 gccattctct ccggctgccg ggccgaggag ctctacagct ggatcgcccg cggcgacgaa
     5701 gccgccctca cccgcatccc cggcctcgga aaaaagaccg cccagcgcat gatcctcgac
     5761 ctcaaggaaa aggcggccca gcagctgcaa aagggcggta tcacggccgc cccggctccc
     5821 gctctggcct ccgggctctc cgaacaggct cttcaggcct tgaccgggct cggtttcagc
     5881 aggctcgagg cgcaaaaggc cctggaaaaa gccgcggccc ggctgggaga gggtgcggaa
     5941 ctggaagagt tgctgcgttc agccctgcag ggctgagctg aaggagcatg gtgaatcaaa
     6001 gcgaccgccc cacatatccg gaagccctga acggcgaacg ggaattcgac gcgaccctgc
     6061 gcccggagcg gttcggcgaa ttcatcggcc aggacaagct caaggagaac ctccatatct
     6121 tcattcaggc ggcccgggcg cgccaggagg ccctcgacca cgtcctcttt tacggcccgc
     6181 ccggcctcgg caagaccacc ctggcccata tcatcgccag ggagatgggc agccagatcc
     6241 gcctgacctc cggcccgacc ctcgagcgcg ccggcgacct cgccggcctg ctcaccaatc
     6301 tcggggagca cgacgtcctc ttcatcgacg aaatccaccg cctcaaccgc gtcgtcgagg
     6361 agtatctcta cccggcgatg gaggatttca agctcgatat tatcctcgac aagggggcca
     6421 acgcccgctc gatccagctc aaactgcccc atttcaccct tgtcggggcc accacccgcg
     6481 ccggcctgct cacctcaccc ctgcgcgccc gcttcggggt ctcgtttcgc ctcgatttct
     6541 atgagcccga acggctctgc gagatcgtcc tgcgctcggc caggatcctc ggcaaaaaga
     6601 tcgatccggc cgccgcgctc gaaatcgccc ggcgctcccg cggcaccccg cgcgtggcca
     6661 accgcctgct gcgccgcatc cgcgactttg cgcaaatcct cggcgaggag gagatcaccc
     6721 ttgaacgcgc cgcctcggct ctgcagcgcc tcgacgtcga tgagagcggc ctggatgaga
     6781 tggacaaacg catcctcctg accgtgatcg ataaattcag cggcgggccg gtcggactca
     6841 acaccctggc cgtcgccatc ggggaagagc ccgataccat cgaggagctc tacgaacctt
     6901 atctgatcca gtccggcttt ctcaaccgga cgccgcgcgg acgcaccgcc accgacgcgg
     6961 cctataccca tttccaccgc cgtcacggcg gcgcccgtca gcagcagctg ttcgactagc
     7021 agccgcagcc cctgcgggct ttttcttgaa atgagccttt gctctatctc gaggaatccg
     7081 gtgcgatccg gattctgcac tctacagagg aggaatcgcc gcgatgaaat tgtccgattt
     7141 caagtataat ctgcccgaca aactgatcgc ccagtacccc agcgacgagc gtgaccagtg
     7201 ccgcctgatg atcctcaacc gcgagagcgg caccatggag gagggcattt tcgccgatgt
     7261 catcgattac atggagaagg gcgacagcct ggtcatcaac cagaccaagg tcttcccagc
     7321 ccgcctcgaa gggagcaagg acaagaccga cgcccgcgtc gaggtctttc tcttgcgcga
     7381 gctcgaacag ggactctggg aagtgctggt caaaccggcg cgcaaggtgc gcgtcggcaa
     7441 tcgcctcacc atcggcaacg aactcgcctg cgacgtcatc gacaacaccg tctcgggcgg
     7501 gcgcgtggtc cgcttcaatt acagcggcga tttttacgag atcgtcgacc gcatcggcaa
     7561 gtcgcccctg ccgccctata ttcgccgcga gccggaaccg agcgacaagc tgcgctatca
     7621 gaccgtctac gccagggaac gcggtgcggt cgccgcgcca accgccggcc tccacttcac
     7681 cgagcccctg ctcaaaaaga tcaaggccaa ggggatcaag atcgtgcccc tcaccctcca
     7741 cctcgggttg ggcagcttcc gcgccgtggt ggtggaggat ctcagccgcc acaagatgga
     7801 ttccgaattc ttcgaaatct cggaggaggc ggccgaggcg atcaatgaga ccatgcgcag
     7861 caagcacaag gtcgtcgcgg tcggtaccag tgtggtgcgc gccctcgagg ccaacgtcac
     7921 ctccgaaggc tgggccaagg ccgggcacgg ctggaccgac aagttcatct atccgcccta
     7981 tgaattcagg attgtggacc ggatgatcac caatttccac ctgccccagt ccaccctgct
     8041 catgctggtc tccgccttcg ccggccgcga cctcgttttc aaggcctatc gcaaggcgat
     8101 ccgcgacaag ttcatgttct tcagctacgg cgacagcatg ctcatcctct agccagccgg
     8161 cgctgtcgcg atggaaaaga gaacaaaacg ggtggccctc atcgtcgccg ccggacaggg
     8221 caaacgcttc ggcggcgcgg tgagcaagca gttcgtcgag gtgggcggcc ggcccctgct
     8281 ctggtacacc ctgaccgctt ttgagcgctg ccccgatatc gacgagatcc tggtaatcct
     8341 cccggccggc gagctgggcg agcgcaccgc ggagatcgcc gcctggggcc tgaccaaatt
     8401 ttgtgccgcc gtggcggggg ggaaggagcg ccacgattcg gttttcaacg gcctgcaggc
     8461 cctcagctca caggccgggc tggtcgccat tcatgacggc gcccgccccc tgatcacccc
     8521 ggagctcatc tccgcctgcc tgcgtgcagc ggaggagtgc ggcgccgcct ttgccgggat
     8581 cgcacccaag gacaccatca aggaggtgga gggggggctg gtcaccgcca ccctcgaccg
     8641 ccgccgcctg gtgctggtgc agacgcccca ggtcttccgg gtcgatcttg tccgccacgc
     8701 ctatgattcg gcctttgagc gcggcgcctt cagcaccgac gacgccgccc tggtcgaggc
     8761 cctcggccat ccggtgcggg tcgtgcccgg ggattaccgc aacctcaagg tcacctcgcc
     8821 cgaagatctg ctcttcgtca aggcctgcct ggagatctag tccatcaggg agaagttcat
     8881 gcgcatcggc catggttttg atgtccaccg cctcgtctcc ggccgcaagc tcatcctcgg
     8941 cggggtcgag atccccttcg agctgggact gctcggccac tccgacgccg acgtgctctg
     9001 ccacgccgtc agcgacgccc tgctcggggc cctg
//
LOCUS       DAOPWU010000126         8868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7907_length_8868_cov_4.635992, whole genome shotgun sequence.
ACCESSION   DAOPWU010000126 DAOPWU010000000
VERSION     DAOPWU010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7907_length_8868_cov_4.635992"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKM23_07940"
     CDS             <1..253
                     /locus_tag="PKM23_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91444.1"
                     /translation="SLGSYPNPCNGRTTLDVTLAQAVTVRLQIFDLLGREMARLADGP
                     LPAGPHRFTLDAAVWPSGIYWARLTAGAQQQTLKLLLAR"
     gene            405..605
                     /locus_tag="PKM23_07945"
     CDS             405..605
                     /locus_tag="PKM23_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91445.1"
                     /translation="MSAIYLIVKLPAGHLVMPLLALASLRVLLHGMLSHPGFGLLKGA
                     IVTLLAALATTFLIRRQIFWKS"
     gene            616..1752
                     /locus_tag="PKM23_07950"
     CDS             616..1752
                     /locus_tag="PKM23_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016868394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNY91446.1"
                     /translation="MRPLPLLLCLTLLLPAAGLEQEPQAPPPELRGVWLTDVDSKVLF
                     SRKRIAEAMTFLAAHHFNVVFPVVWNQGYTLYPSAIMDSLFGIPIDPAHRGRDPLAEL
                     IEEAHRHGLKVIPWFEYGFSSSHRAQGGHLLAAKPHWAARDRAGKLLTKNGFEWMNPY
                     HPEVQEFLAGLVLEVAARYDVDGIQGDDRLPAQPSEGGYESYTDSLYRSRHEDAAPPA
                     DHRDPEWLRWRADLLNAFACRLYRQVKAVDSTLIVSWSPSIYPFSYEEYLQDWPAWIK
                     GGYADLVLPQVYRYDLEAYRSALLETRAVAPPEAGGRIYPGILIKLGKYLIDPDYLLE
                     AIRLNRELGYRGEVFFFYEGLRKQGGKLARLLLEGPYREPARPF"
     gene            1819..3684
                     /locus_tag="PKM23_07955"
     CDS             1819..3684
                     /locus_tag="PKM23_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNY91447.1"
                     /translation="MHILQSDIFIAGGGLGGCAAALRAAEWGCTVILAEACDWIGGQA
                     VSQGVSALDEHEWIETFGGTASYYRFRSAIRRHYRSLALQDKDNPHFNPGGCWVSRLA
                     FEPPVGLAVLQGMLAPYLRTGQVQLLTRHGLRRARVERERIREVILAPEEGGAELACR
                     SACYIDASELGDLLPLAGVPVVTGAEARSDTGEPHAPAEAHPEWMQSFTFPFIVAFCP
                     GENHTIPKPPGYEENRLHQPYSLAAGKGAQGRPLIYRVFAQTPGTPGSFWTYRRLIEA
                     EKFRPEIYPHDLSLINWHSNDYRGAPLVGVSAAARRQALAGARALSLGFLYWLQTEAP
                     RDEGGLGYPELQLRPELMGSADGLAQFPYIRESRRIAALTRLCEQDIAAEMQPHARAR
                     LFSDSVAIGNYWIDIHKSGGEEADLFSDTRPFQIPLGALLPQGCTNLIAGAKNIGTTH
                     ITNGACRLHPVEWAIGEAAGALAAESLRRQCTPVEIHARPAARLALQLRMAESGTPLF
                     WFIDVGQTHPAFAALQWLAVAGIITAEPESLLFNPEAQLDAETASAWLEAAGEWWGIR
                     QRVWPRAAAKAERPWHPLAALAQDGSQKDMTRARFALALFESLKGLLHSLHEAHA"
     gene            3681..5168
                     /locus_tag="PKM23_07960"
     CDS             3681..5168
                     /locus_tag="PKM23_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HNY91448.1"
                     /translation="MIATLQLIGFLVVFVVLALLMFFRKLPALIALPLMAVLTALLGG
                     VRGADLLHHVIGGGAVRLHTAYTVAIFGGMLSFLLQKSGVAEQLIKNGAELAGDRPWS
                     VGLIMLLLIAMLFTTLGGLGAIIMVATIVLPIMASVGFSSLTIAGIFLIGINLGGILN
                     PGNWALYIDVLKLDIPTIRSFALVLFAICLLMALAFLTLELRREGHKLELKKPLRYGL
                     LIAVCTIFLGWGWQQLQPLTVWKFIWRTIGAGFRSTLLLAALLLAGRILFDLARKWSG
                     RERHMSRIVWYAYLTPVVPLLLILIFKIHFVTAFIAGLFYGFVVTWRKGSINMLSQAI
                     IEGGASVMPAVVLMMGIGMLLTAIIGPGGDWSALNGGAEWPVLAFLKPVMVEIIPSSP
                     WLYVLVFTLLAPLSLYRGPLNVWGMGYGMAAIMLASGALPAAAIMGLLISVGQIQGIC
                     DPTNTQNVWIANELHLDVQEILWRTLPYVWVVSALGLTAAAFLFF"
     gene            5183..7333
                     /locus_tag="PKM23_07965"
     CDS             5183..7333
                     /locus_tag="PKM23_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNY91449.1"
                     /translation="MQRVIKIGIDVGGTFTHAVAVNAVRHAIIAKSCVPTTHTAAEGV
                     ARGVVEAMEKLLEAGSIAAEEVVLIAHSTTQATNALLEGDVAQVGVIGMASGIESGRA
                     RRETDVGDIELAPGKFIRTRHRFLDSSSMTEEHIRALIGELVEEGAEVVVASESFGVD
                     HPENEERVVAAAAAMGLPASAASMISQLYGLRIRTRTAVINASMLPRMLETANSTERS
                     VRASGINAPLMVMRSDGGIMEIDEMRRRPILTMLSGPAAGVAAALMYARISDGIFIEV
                     GGTSSDISAIRNGRPLVRSAVVGGHKLYLRTLDVRTVGIGGGSMPRIARNRIVEVGPR
                     SAHIAGLKYPAFSGIPEGAKFVQRLVQPKAGDPDDYLRIEIVGDAEAYTLTPTEAASH
                     LDLVPEAGYGRGSRPAVGRLFAWLEKEWGEEGIKIATRLLDLAAAKVMAVVEQLIREY
                     RMERSLVQLVGGGGGAPAIVPYTAQAMQLPYQIAENCEVISAIGAALGIIRDSVERTL
                     INPTAEDLLRLRSQAIASVQRMGASPDSIEVTIEIDRQSKRVTATATGASELRARDLG
                     LEPLSPEALHALAARALGCDPEAVTAAGGTPLLNLFMCRRISTRLWGLIRREQKAVRV
                     LDQEGIIRLQLADADFWQGTIASARSFIEEAIENLTIYGDAGALLPDLFLLIGRRIID
                     LTGVAEKEQILALARIETEKYGPDEPLVVLAAAR"
     gene            7359..8789
                     /locus_tag="PKM23_07970"
     CDS             7359..8789
                     /locus_tag="PKM23_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNY91450.1"
                     /translation="MTQSQSSIRFGTDGWRALIAETFTFENVRTVGQALADELIAAQQ
                     VGRGVAIGYDTRFLSGRFAEALAGVLGANHIPVLLSDAFCPTPLLSFAVRERGLAAGI
                     MVTASHNPWHYNGIKFKSEWGGPFMPEQTRAVESRLFRTAVRRDEALARKLVEPTDFF
                     APYARHIGDYFDWTRITASPLRLVCDAMHGAGCGFLRRLLPPGWGESRFIGIGQNPHP
                     LFANRLPEPILVNLGALAAAVPAHGADLGLATDGDADRFGVLDHQGRFVELHDLMPLF
                     FRHLVTSRGWPGDVVRTTSMAATIDRMAAAAGRRVIEVPVGFKHVAEEMLRQEILIGG
                     EESGGFGYHGHLPERDGLFSCLMTLELLAASGATLAELVQQLRREYGPFAYGRIDHPY
                     APEILHKNMQTLRQEPPGRIGRFKVASINPVDGIKFYFDEGSWMLLRLSDTEPLGRIY
                     AGSERQDKVQQLLEEGKNLLTQPPPR"
BASE COUNT         1450 a         3140 c         2687 g         1591 t
ORIGIN      
        1 cagcctcggc agttatccca atccctgcaa cggccgcacc acactcgacg tcaccctggc
       61 gcaggccgta acagtccggc tgcaaatctt tgacctgctc ggccgggaga tggcccgcct
      121 cgccgacggc cccttgccgg ccggtccgca ccgcttcacc cttgatgccg ccgtttggcc
      181 cagcggtatc tattgggcgc gcctcactgc gggtgcgcag cagcagaccc tgaaactact
      241 cctcgctcgc taggcagggc gcatggacaa acgcgcgcaa gctctcgacg gcctgcgggg
      301 cctggccgtc atcggcatga tcctctccgg ccaggtcccc tgctccggac catccgccct
      361 tcacccgcca gctgaccctc aatctggcgg gaaatggttt gcttttgtcc gcgatttatc
      421 ttatcgtaaa gctgcccgcc ggccacctgg taatgccctt gcttgccctc gcctcgctga
      481 gggttttgct ccacggcatg ctgagccatc ccggatttgg cttgttgaag ggtgcgatcg
      541 tcaccctgct ggccgcactc gcgaccacat tcctgatccg caggcaaatt ttctggaaaa
      601 gctgaaagga gatccatgcg tcccctcccc ctgctgctct gtctgaccct cctgctgccg
      661 gcagccggcc tcgagcagga gccccaggca ccgccgcccg aactgcgcgg ggtctggctg
      721 accgatgtcg acagcaaggt gctcttcagc cgcaaacgca tcgccgaggc gatgacattc
      781 ctcgctgccc accactttaa tgtggtcttt ccagtggtct ggaaccaggg ctacaccctc
      841 tatcccagcg cgatcatgga ctccctcttc ggcatcccca tcgatccggc ccaccgcgga
      901 cgcgatcctc tcgctgaact gatcgaggag gcgcatcgcc atggcctcaa ggtgatcccc
      961 tggttcgaat acggcttctc ctcctcccac agagcccagg gcggccatct cctggccgcc
     1021 aaaccccact gggccgcccg cgaccgcgcc ggcaaactgc tgaccaagaa cggtttcgaa
     1081 tggatgaatc cctaccatcc cgaggttcag gaattcctcg ccggcctggt gctggaggtg
     1141 gcggcccgct atgatgtcga tggcattcag ggcgatgacc gtctgcccgc acagcccagc
     1201 gaaggcggct atgaaagcta caccgacagc ctctatcgca gccggcacga ggacgcggcg
     1261 ccgcccgccg atcaccgcga tccggagtgg ctgcgctggc gcgccgatct gctcaacgcc
     1321 tttgcctgcc gcctttaccg gcaggtcaag gccgtcgatt ccactctgat cgtctcctgg
     1381 tccccctcga tttacccctt cagctatgag gagtatctcc aggactggcc ggcatggatc
     1441 aagggcggct atgccgatct ggtgctgccg caggtctacc gctatgatct ggaggcctat
     1501 cgcagcgccc tgctggagac gcgggcggtg gcgccgcccg aagccggcgg gcgcatctat
     1561 ccgggcatac tgatcaagct gggcaaatat ctcatcgatc ccgactatct gctcgaagcc
     1621 atccggctca accgcgagct gggctaccgg ggcgaggtct tttttttcta cgagggactg
     1681 cgcaagcagg gtggcaagct ggccaggctg ctgctggagg gaccctaccg tgagccggcc
     1741 cggccctttt aagcctgcgc cgccccgatc acttataaat cgaccaagcc tgtagcaaga
     1801 tcgagaggac gattccggtt gcacattctg cagagtgaca tctttatcgc cggaggggga
     1861 ctcggaggct gcgccgccgc cctgcgcgcc gcagagtggg gctgcaccgt catcctcgcc
     1921 gaagcatgtg actggatcgg cggccaggcg gtcagtcagg gggtctcggc cctcgatgag
     1981 cacgaatgga tagaaacctt tggcggcacg gcctcctact atcgctttcg cagtgccatc
     2041 cgccgccact accggagcct cgccctgcag gacaaggaca accctcactt caatcccggc
     2101 ggctgctggg tgagccgcct cgctttcgag cccccggtcg gtctggctgt gctgcagggg
     2161 atgctggccc cctacctccg cactggtcag gttcagctct tgacccgtca tggtttgcgc
     2221 cgggcgagag tggaacgtga gcgcatcaga gaggtgattc tggcgccgga ggagggcggt
     2281 gcggaactgg cctgccgctc cgcctgctat atcgacgcca gtgagctcgg ggatctcttg
     2341 ccgcttgcgg gagtgcccgt tgtcaccggc gccgaggcgc gcagcgatac cggcgaaccg
     2401 cacgccccgg cggaggctca tccggagtgg atgcaatcct tcaccttccc cttcatcgtc
     2461 gccttttgtc ccggcgagaa tcacacgatt cccaaaccgc cgggctatga ggagaaccgg
     2521 ctgcatcagc cctacagcct ggctgcgggc aaaggggcac aggggcgccc gctgatctac
     2581 cgcgtgttcg cgcagacccc cggcacaccg ggcagttttt ggacctaccg ccgtctcatc
     2641 gaagcggaaa aattccggcc ggagatctat ccccacgacc tgagcctgat caactggcac
     2701 agcaacgatt accgcggggc gccgctcgtc ggcgtcagtg cggcggcgcg ccggcaggcc
     2761 ctggccgggg cgcgggcgct cagcctcggc tttctctact ggctgcagac cgaggccccg
     2821 cgcgacgagg ggggcctggg ttatcctgaa ctccagctgc ggccggagct gatggggagc
     2881 gccgacggac tggcgcagtt tccctatatc cgcgagtcgc ggcgcatcgc cgccctgacg
     2941 cggctctgcg agcaggatat tgctgctgag atgcagccgc acgcccgcgc ccgacttttc
     3001 agtgacagcg tggccatcgg caattactgg atcgacatcc acaaaagcgg cggcgaggag
     3061 gcggatctct tcagcgacac ccgccctttc cagatcccgc tgggggcgct gctgccgcag
     3121 ggatgcacaa atctcatcgc cggcgccaag aacatcggca ccacccatat caccaacggg
     3181 gcctgccgcc tccatccggt ggaatgggcg ataggcgagg cggccggcgc gctggcggcc
     3241 gaatcgctgc gccggcagtg cacccctgtg gagattcacg cccggccggc ggcgcggctc
     3301 gccctgcagc tgcgcatggc cgaatcgggc acccccctct tctggttcat cgatgtcggc
     3361 cagacccacc ccgctttcgc tgcgctgcag tggctggcgg tcgccggcat catcacagcg
     3421 gagccggaga gcctcctctt caatcccgag gcgcagctcg atgcggaaac cgcctcggcc
     3481 tggctggagg ccgcagggga gtggtggggc atcaggcagc gggtgtggcc ccgcgccgcg
     3541 gcgaaagccg aacgcccctg gcatcccctg gcggccctgg cacaggatgg cagccaaaag
     3601 gacatgaccc gggcccgctt cgccctcgca ctcttcgagt ccctcaaggg actccttcac
     3661 tccttacatg aggcacacgc atgatcgcca ccttgcagct catcggcttc ctcgtcgttt
     3721 tcgttgtcct ggccttgctg atgttcttcc gcaagctgcc cgccctcatc gccctgccgc
     3781 tcatggctgt gctgacggct ctcctcggcg gggtccgggg agccgatctg ctccatcacg
     3841 tcatcggcgg cggcgccgtg cgcctgcaca ccgcctacac cgtggccatc ttcggcggca
     3901 tgctcagctt cctgctgcag aaatcggggg tggccgagca gctgatcaag aatggcgctg
     3961 aattggccgg cgaccgcccc tggagcgtcg gtctgatcat gcttttgctc attgccatgc
     4021 tcttcaccac cctgggcggc cttggcgcca tcatcatggt ggcgaccatc gtcctgccga
     4081 tcatggcctc ggtcggcttc agcagcctca ccatcgccgg catcttcctc atcggcatca
     4141 acctgggcgg catcctcaat cccggcaact gggcccttta tattgacgtc ctcaagctgg
     4201 atattcccac catccgcagt ttcgccctgg tgctcttcgc gatctgcctg ctcatggccc
     4261 tggcttttct caccctcgag ctgcgccgtg aggggcacaa gctggagctc aaaaaaccac
     4321 tccgctacgg ccttctcatt gccgtgtgca ctatattcct gggctggggc tggcagcagc
     4381 tgcagccgct gacggtttgg aaatttatct ggcgcaccat cggcgccggc ttccgctcga
     4441 ccctgctcct ggcggcgctg ctgctggccg gccgcatcct cttcgacctc gcccgcaagt
     4501 ggagcggccg cgagcgccac atgagccgca tcgtctggta tgcctatctc accccggtgg
     4561 tcccgctgct gctgatcctg atctttaaaa tccacttcgt caccgccttt attgccggac
     4621 ttttctacgg ctttgtcgtc acctggcgca agggcagtat caacatgctc agccaggcga
     4681 tcatcgaggg cggggccagc gtcatgccgg cggtggtgct gatgatggga atcgggatgc
     4741 tcctcacggc catcatcggc cccggcggcg actggagcgc cctcaatggc ggggcggagt
     4801 ggccggtgct tgcctttctc aaaccggtga tggtcgagat catcccctcc tccccctggc
     4861 tctatgtgct ggtcttcacc ctgctggccc ccctctcgct ctatcgcggc cccctcaacg
     4921 tctggggtat gggctacggc atggcggcga tcatgctcgc ttccggcgcc ctgccggccg
     4981 cggccatcat gggactgctg atttccgtcg gccagatcca gggcatctgc gacccgacca
     5041 acacccagaa cgtctggatc gccaacgagc tccatctcga cgtccaggag atcctctggc
     5101 gcaccctgcc ctatgtctgg gtggtctccg ccctgggatt gacggcggcc gcatttctct
     5161 tcttctgaaa aaggtcaagc ccatgcagcg tgtgatcaag atcggaatcg atgtcggcgg
     5221 caccttcacc cacgcagtgg cggtgaatgc ggtgcgccac gccatcatcg ccaagagctg
     5281 cgtgcccacc acccacaccg ccgccgaggg ggtggcgcgg ggggtggtcg aggcgatgga
     5341 aaaactgctc gaggcgggca gcatcgccgc cgaagaggtg gtgctgatcg cccacagcac
     5401 cacccaggcc accaatgccc tgctcgaagg ggatgtcgcc caggtgggtg tgatcggtat
     5461 ggcgagcggc atcgagagcg gccgcgcccg gcgcgagacc gacgtcggcg atatcgagct
     5521 tgcccccggc aagttcatcc gcacccgcca ccgctttctt gacagcagca gcatgaccga
     5581 agagcacatc cgcgccctca tcggcgaact ggtggaggag ggcgccgagg tggtcgtcgc
     5641 cagtgaatcc ttcggagtcg accaccccga aaacgaggag cgggtggtcg ccgcggcggc
     5701 ggcgatgggg ctgccggcca gtgccgcctc gatgatctcg cagctctacg gtctgcgcat
     5761 ccgcacccgc accgcggtga tcaatgccag catgctgccg cgcatgctcg aaaccgccaa
     5821 cagcaccgag cggagcgtgc gcgcctccgg catcaacgcc ccgctgatgg tcatgcgcag
     5881 cgatggcggg atcatggaga tcgacgagat gcgccgccgc cccatcctga ccatgctctc
     5941 cggccccgcc gccggcgtgg cggcggccct gatgtacgcc cgcatctcgg acggcatctt
     6001 catcgaagtg ggaggaacca gctctgacat ctcggccatt cgcaacggcc ggcccctggt
     6061 gcgcagcgct gtcgtcggcg gccacaagct ctatctgcgc accctggacg tccgcaccgt
     6121 cggcatcggc ggcggctcga tgcccagaat cgcccgcaac cgcatcgtgg aggtcggccc
     6181 gcgcagcgcc catatcgccg gactcaagta ccccgccttc tccgggatac ccgaaggagc
     6241 caagttcgtg caaaggctgg tgcagcccaa agcgggcgac cccgacgact atttgcgcat
     6301 cgaaatcgtg ggcgatgcgg aggcctatac cctcaccccc accgaggcgg ccagccatct
     6361 cgacctggtg ccggaggccg gctacggacg cggcagccgg ccggcggtcg gccgcctctt
     6421 cgcctggctg gaaaaggagt ggggcgaaga gggaataaag atcgccaccc gcctgctcga
     6481 cctggctgcg gccaaggtga tggctgtggt cgagcagctg atcagggagt acaggatgga
     6541 gcgcagcctg gtgcagctgg tcggcggggg cggcggcgca cccgccatcg tcccatatac
     6601 cgcgcaggcc atgcagctgc cctaccagat cgccgaaaac tgcgaggtga tctcggccat
     6661 cggcgccgcc ctcggcatta tccgcgacag cgtcgaacgc accctcatca acccgacggc
     6721 ggaggacctt ctccgcctgc gcagccaggc gatcgcctcg gtacagcgca tgggtgccag
     6781 ccctgattcg atcgaggtca ccatcgagat cgaccgtcag agcaagcggg tcaccgccac
     6841 cgccaccggc gcctcagaac tgcgcgcgcg cgacctcggc ctcgagcccc tcagccccga
     6901 agccctgcac gctctggcgg cccgggctct cggctgcgac ccggaggcag tgaccgcggc
     6961 cggtggtacc cccctgctca atctttttat gtgccgccgg atttccaccc gcctctgggg
     7021 gttgatccgc cgcgaacaga aggctgtccg cgttcttgac caggagggca tcatccgcct
     7081 gcaattggcc gacgccgact tttggcaggg caccatcgcc tcggcccgga gctttatcga
     7141 ggaggccatc gaaaacctca ccatctatgg cgatgccggc gccctgctcc ccgatctctt
     7201 tctcctcatc ggacggcgca tcatcgatct caccggcgtg gccgaaaaag aacagatcct
     7261 cgccctggcc cggatcgaaa ccgagaaata cggcccggac gagccccttg tggtgctcgc
     7321 cgccgcgcgt tgatgcattt gcccaaggtc cgctctttat gactcaatcc caatcctcca
     7381 ttcgcttcgg caccgacggc tggcgcgccc tcatcgccga aaccttcacc tttgaaaatg
     7441 tgcgcaccgt cggccaggcc ctggccgacg aactcatcgc cgcacaacag gtcggccgcg
     7501 gggtggcgat cggctacgac acccgtttcc tctccggccg cttcgcggag gcgctcgccg
     7561 gcgtgctcgg cgccaatcat attccggtgc tgttgagcga tgccttttgt ccgacgccac
     7621 tgctctcctt cgccgtgcgc gagcgtggcc tggccgccgg gatcatggtg accgccagcc
     7681 acaatccctg gcattacaac ggcatcaaat tcaaatcgga gtggggcggg cccttcatgc
     7741 cggagcagac ccgcgccgtc gagtcccgtc tcttccgcac ggcagtgcgc cgggatgagg
     7801 ccctggcgcg caagctggtc gagccaaccg atttttttgc cccctacgcc cgccatatcg
     7861 gtgattattt tgactggacg cgcatcactg cctcccccct gcgcctggtc tgcgacgcca
     7921 tgcacggggc cggctgcgga ttcctgcgcc gcctgctgcc gcccgggtgg ggggagagcc
     7981 gctttatcgg catcggccag aatccccatc ccctctttgc aaaccggctg cctgagccga
     8041 tcctcgtcaa cctcggcgcg ctggcggcgg ccgtgccggc gcacggggcc gacctcggcc
     8101 tcgccaccga cggcgacgcc gaccgcttcg gcgtcctcga ccaccagggc cgcttcgttg
     8161 aactgcacga cctcatgccc ctcttcttcc gccatctggt cacgagccgc ggctggccgg
     8221 gcgatgtggt gcgcaccacc tccatggccg ccaccatcga ccgcatggcc gccgctgcgg
     8281 gccgccgcgt catcgaggtc ccggtcggat tcaagcatgt ggccgaagag atgctgcgcc
     8341 aggagatcct catcggcgga gaggagagcg ggggattcgg ctaccacggt cacctcccgg
     8401 aacgggacgg cctcttttcc tgcctcatga ccctggaatt gctcgccgcc tccggcgcga
     8461 ccctggccga actggtgcag cagctgcgcc gcgaatacgg ccccttcgcc tatggccgca
     8521 tcgaccaccc gtacgccccg gaaattctgc acaagaatat gcagacactg cgtcaggagc
     8581 cgcccggccg gatcggtcgc ttcaaggtcg cctcgatcaa cccggtcgac ggcatcaagt
     8641 tttacttcga tgaaggctcc tggatgctgc tgcgcctctc cgataccgaa cccctcggcc
     8701 gcatctatgc cggttcggag cggcaggaca aggtgcagca gctgctcgag gaaggcaaga
     8761 atctgctcac acaacccccc ccacgataag gaaaagaact gtgcataaat acgcccattt
     8821 ttccgatgac cggcgcgaga tcatcatcac ccggccggac actccggc
//
LOCUS       DAOPWU010000127         8855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_7922_length_8855_cov_4.712159, whole genome shotgun sequence.
ACCESSION   DAOPWU010000127 DAOPWU010000000
VERSION     DAOPWU010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7922_length_8855_cov_4.712159"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PKM23_07975"
     CDS             <1..1138
                     /locus_tag="PKM23_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91451.1"
                     /translation="MLEVLFRGPFNQSPPALNTLPQLLREAVTFFNQAERGGEIWLLT
                     DASRHCDPLATAMEIIQQTHGRAVKPIVFRIISADLGYNTYQTINSQVYYGNDYLYEN
                     LARSSWGTFVRLRSAPWYDYLDLMLDAAAPTVNTVEIDPEPAGGLAYTRFPLNQGRAN
                     FPIALPYYEIGLYDGTTPFDVHFYGSFQGELFARTVTIDRQAEDPGWQAVALCWFDRY
                     IQNLLLEPQSHETITYIEQTSVENRLLTPYSGFIVPGADGLLAFARLEEGTSSVVCRP
                     EESAAETPRRCELSAFPNPFNAATTLDISLPELLESETVEVRIINFLGQTVHEHRFEA
                     SAGTRLQLVWDGLDPAGRPVGSGIYLVIVRAGSLMKNIKISLIK"
     gene            1151..3346
                     /locus_tag="PKM23_07980"
     CDS             1151..3346
                     /locus_tag="PKM23_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91452.1"
                     /translation="MRRIIIILLMVSTAFAADFCVYEANYPNVKDYNVRVDAADLTII
                     PRGAFLEMTIELTVSYDFNSWYFKNYDELEFMWEFTLPDQASITGFWLWQEDSLLQAT
                     LMDKWTAELLFSEVSSPVRNPALLTRSFADREGQVHYNVRLYPVTRAVKRTFKLEYLL
                     PARPTNETLRVWLPITQLTSRLSPGARELKIHFKSSEPPELLGADAGSGSFNPAADCW
                     DHTIQLDYDMFVELIYPTPIEGKHFFSTFEKNGEAYYQLAVYPPEQEVQRTPRNFLIL
                     VDFNRFNTKGLDGELVLMLLKETMQQALTEHDSVNILVAFDELVWGAAEFRACTEENL
                     DTLFQKVLQRSFPSYSNFQALISSAAAFLQSKKGPVDLLLFSNTDEIALPYQLIDSGR
                     EELAAQIVAMMPAGTRIHVVDLENQSSLLYSSQESQYLAQLQTFYAFMCNKTGGNLFF
                     LRYHALKNILSALFYEHITHYQEIEVQTRFASGYAWGKHLLALHQGYYPLHFPVMQIG
                     RFQGALPLEITVLGKTRLDKFSDQFTITASEVIPGDEKLALAWHGNHIHELLQNRQTN
                     ATIMEIMDTSLNHGIVTPYTSFIVLRPDQEHGYDPLQIAQNKESGSSGRGEYDNEVPS
                     STGVLDHTGAEDATFCEVRAYPNPFNISVTLDLLLPELIASEDLTLTIYNMLGQKIRE
                     FRMEKQGGRSMLLRWDGRDEHGQPAGSGVYLAVLRGSGIYRSTKLVLMK"
     gene            3772..4017
                     /locus_tag="PKM23_07985"
     CDS             3772..4017
                     /locus_tag="PKM23_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91453.1"
                     /translation="MAPALPAAEESTPVLVQPDCCELVSLYHRVEESAPPAFTALLQA
                     PAAPIALLPPNRSSAAGIVCCTATVHTAERLNLPLLI"
     gene            4115..6328
                     /locus_tag="PKM23_07990"
     CDS             4115..6328
                     /locus_tag="PKM23_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91454.1"
                     /translation="MNKLILFLFFWAGAAALEAQPRDTWTCGHVYGIELHGAHQDTIP
                     LIHANVMWAGTTLGTVSDDEGFFKLDLAPASKKLVAGYVGYESDTLVVEPGMHGLAFY
                     LEPDDHHEEMTIEGKKPHTLHLHEAEVNTQSITRQGLRTLACCNLAESFENSLSVDVE
                     QSDAVSGAKRIKMLGLAGFYTQILVEKNPLMRGLISPFGLEYIPGFWIDAIDISKGTA
                     SVATGYESITGQINVELAKPEDSRPLALNAYQNSMGRSELTLSAARKVTPKLSTMLLS
                     YGSYNRQRWDEDGDTFLDMPLVSHISLMNRWKYHSSSGDGQIGFKILRDTRDGGQTGF
                     DFGRPRAANSGYGSRNRIGRYEFFAKAGTAFGGGRSIGLILSGFHHDQDSFWGAKGYN
                     GLEQSLYANLIFEKEAAHHRFSSGLSFLLDERDETYQEQLFKTRERVPGLFAEYTWKP
                     NDRLSAMAGFRYDNHNLYGGFYTPRLHLKYQADALTALRLSAGKGYRVPHLFMDNPAM
                     LASSREVIFAESLEAEEAWNAGMQLTREFSLGQDRPGTLALDFYRTEFLQQTVVDLDR
                     DPRTILLYNLDGRSFSNSAQIEVTATAARGLDLTAAWRFNDVRMTYAGELKEVPLNPR
                     HKGVLVLSWSPTGRKWQFDVTTQYNGATRLPETALNPEPYRMGSHSPAYGQLFAQVTR
                     KFGKMDLYLGGENLTGFRQERPILAWDDPFSPWFDSSLVWGPTAGRRFYVGLRVN"
     gene            complement(6345..7280)
                     /locus_tag="PKM23_07995"
     CDS             complement(6345..7280)
                     /locus_tag="PKM23_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNY91455.1"
                     /translation="MKKALLALFLIIASLAPAQERDLAQEVDDRFNFLIANDLGRNGY
                     YDQKPVAAKMGEVGEKVDVEFVALLGDAHHFNGVASVQDPLWMTNYELIYNHPELMCE
                     WYPVIGNHEYRGNTQAVLDYRKVSRRWAMEGRYYTKVFAVDDSGATLRLVFIDTPPLI
                     DKYLNNHEEYPDAGKQDLQRQLTWIDSVLTVNTATWTMVMGHHPVYAQTTKTEDERRD
                     MQQRLDPLLRKHKVDIYICGHIHSHQHIRRADSPVDYIVNSSASEFRKVSPIEGTRFC
                     AAASGFSVCSVSAADLRVYIMDKEGKVLHVVERKK"
     gene            complement(7483..8295)
                     /locus_tag="PKM23_08000"
     CDS             complement(7483..8295)
                     /locus_tag="PKM23_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNY91456.1"
                     /translation="MTGFSPLRPFGSTGIMVSPLGLSSSYRPGKHAVQLASDEGINLF
                     FAFGIDTQMTGGLRQILPARREQLVLATGAYNYIWWAQNIRKTCEKRLRQFKTDYIDI
                     FLFLGVMKPAEFPQKVRDELLKLKEEGKVRAVGLSCHNRLFLGELAASGEMDALMLRY
                     NAAHRGAEQDIFPHLAAHNPGVLGYTATRWSYLVRRPRSLPADARVPTAGECYRFVLS
                     NPHVHCVLTAPRSERELRENIAAVRQGPLQEEELAFMRGFGDVVHGQKRWFM"
     gene            complement(8329..>8855)
                     /locus_tag="PKM23_08005"
     CDS             complement(8329..>8855)
                     /locus_tag="PKM23_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91457.1"
                     /translation="ESITTLSAALVDSWRKVNGSLPGYTVPCDAPNARIDYVFYKSSK
                     IKPLASAVVMKDSFRGNYPSDHAGVMTTFGVDPLGVHISAAGPVHAELLQNYPNPFNA
                     ATVIHYHLLRRGHVTLTIHDLRGRVIEVPVDEVQDAGSHQIRFDAGALPAGLYPYYLV
                     SETGISSGKMVLVR"
BASE COUNT         1783 a         2803 c         2492 g         1777 t
ORIGIN      
        1 catgctggag gtccttttca gaggcccctt caaccagtcc cccccggccc tcaacaccct
       61 gccgcagctg ctgcgtgagg ctgtcacttt cttcaaccag gccgagcggg gaggcgaaat
      121 ctggctgttg accgatgcca gccgccattg cgatcccctc gccacggcca tggagatcat
      181 ccagcaaacc cacggccggg ccgtcaagcc gattgttttc cggatcatca gcgcggattt
      241 gggttacaac acataccaga cgatcaattc ccaggtctat tacggcaatg attatcttta
      301 tgaaaacctg gcccgatcga gctggggcac ctttgtgcgg ctcaggagtg cgccctggta
      361 cgactatctc gatttgatgc tcgacgccgc tgcaccgacg gtcaacacgg tggagatcga
      421 tcccgagccc gccgggggat tggcctacac ccgcttcccg ctgaatcagg gccgggcgaa
      481 ttttccgatc gctcttccct attacgaaat cggactctat gacggcacca cccccttcga
      541 cgtgcatttc tacggctcgt tccagggcga actcttcgcc cggacggtga ccatcgaccg
      601 gcaggcggaa gaccccggct ggcaggcagt cgccttgtgc tggttcgacc gttatattca
      661 gaacctgctg ctcgagccgc agagccacga aaccatcacc tacatcgaac aaaccagtgt
      721 ggaaaatcgt ctgctcaccc cctacagcgg attcatcgtc cccggggcgg acggcctgct
      781 cgccttcgcc cgtctcgagg aggggacatc ctcagtcgtg tgcagaccgg aagagagtgc
      841 ggctgaaacg ccccggcggt gcgaactgag cgccttcccc aaccccttca atgccgcgac
      901 aaccctggat atctcccttc cggagctgct ggagtcggaa accgtggagg tccgcatcat
      961 caactttctc ggccagaccg tgcatgaaca ccggtttgag gcctcggccg gcacccgcct
     1021 tcagctcgtg tgggacgggc tcgatccggc cggccggcct gtcggctcgg gcatctatct
     1081 cgttattgtg cgcgccggca gcctgatgaa gaacattaaa atctcgttga ttaaatagat
     1141 gaggacggcc atgaggagaa tcatcatcat cctgctgatg gtcagcaccg ctttcgccgc
     1201 agatttctgc gtctatgaag ccaattaccc gaacgtcaag gattacaatg tgcgggtcga
     1261 tgcggcggat ttgaccatca taccccgcgg tgcctttctc gagatgacga ttgaattgac
     1321 agtcagctac gatttcaaca gctggtattt caagaactat gatgaactgg aattcatgtg
     1381 ggagttcacc ctgcccgacc aggcgagcat caccggtttc tggctgtggc aggaggattc
     1441 cctgctccag gccaccctga tggacaaatg gaccgcggag ctgctcttca gcgaggtgag
     1501 cagtccggtg cgcaacccgg cactgctgac gcgctccttc gccgaccgtg aaggacaggt
     1561 gcactacaac gtacgcctct atcccgtcac ccgggcggtc aagcgcacct tcaagctcga
     1621 atatctgctg ccggcgaggc cgaccaacga aaccctgcgc gtctggctgc ccatcaccca
     1681 attgacctcg cgcttgagcc cgggagcccg ggagctgaag atccatttca agagcagtga
     1741 accgcccgaa ttgctgggtg ccgatgccgg gtccggatca ttcaacccgg cggccgattg
     1801 ctgggaccat acgatccagc tggactacga catgtttgtc gaactcatct atcccacccc
     1861 catcgagggg aagcattttt tcagcacctt tgagaaaaac ggcgaggcct attaccagct
     1921 cgccgtctat cccccggaac aggaggtgca gcgcacgccg agaaacttcc tcatcctcgt
     1981 cgatttcaac cgtttcaata ccaaagggct ggacggggaa ctggtgctca tgctgctcaa
     2041 ggagaccatg cagcaggcgt tgaccgagca tgactcggtc aacatcctcg tcgcatttga
     2101 cgagctggtc tggggtgcgg ccgaattccg ggcctgcact gaggagaacc tcgacaccct
     2161 cttccagaag gtgctgcagc gttccttccc ctcctacagc aattttcagg cgctgatctc
     2221 ctcggccgcg gcttttttgc agagcaaaaa agggccggtg gacctcctcc tgttcagcaa
     2281 taccgacgag atcgccctgc cctatcagct catcgattca ggccgggagg aactggccgc
     2341 ccagattgtc gccatgatgc cggcgggcac ccgaatccac gtcgtcgacc tcgaaaacca
     2401 gtccagtctg ctctactcct cccaggagag ccaatacctc gcccagctgc agacctttta
     2461 cgcctttatg tgcaacaaaa ccgggggcaa tctctttttt cttcgctatc acgcactcaa
     2521 gaatatcctt tccgcgctct tttatgagca tatcacccac taccaggaga tcgaggtgca
     2581 aacccgcttc gccagcggct acgcctgggg caagcacctg ctcgcgctcc atcagggcta
     2641 ctatcccctg cactttccgg tcatgcaaat cggccgcttc cagggcgcgc tgcctctgga
     2701 gatcactgtg ctcggcaaga cgcgcctcga caagttcagc gatcagttca ccatcaccgc
     2761 aagtgaggtc ataccggggg atgaaaagct ggcgctggcc tggcacggca atcacattca
     2821 tgaactttta cagaaccgcc agaccaacgc caccatcatg gagatcatgg ataccagcct
     2881 caatcacggc atcgtcaccc cttacacctc cttcatcgtg ctgcgccccg atcaggagca
     2941 tggctatgat ccccttcaaa tagcccaaaa caaggaaagc ggcagctccg gacgcggaga
     3001 gtacgacaac gaggttcctt cgtcgaccgg cgtccttgat cacaccggtg ctgaggatgc
     3061 caccttctgc gaagtgagag cctatcccaa tcccttcaac atctctgtaa ccctggacct
     3121 gctgctgccg gagcttatcg caagcgagga tctgaccctg accatttaca atatgctggg
     3181 gcagaaaatt cgggagttca ggatggaaaa acagggcggc cggagtatgc tcttgagatg
     3241 ggacggccgg gatgagcacg gccaaccggc cggcagcggg gtatatctgg ccgttctgcg
     3301 gggttcggga atttaccgga gcaccaagct ggttttaatg aaataagaac cactaacgga
     3361 cccggagaga cattaacaga gggagaagcc ggagcgcatt ctgcggcagc ctctcgccgc
     3421 agtgcacatt tctttttgaa atccggcggt ggattactat ctttacagtg atgatcagtt
     3481 aacttaatta attaattatt aatagaataa aatttgaaaa acctgctgcg caaatctctt
     3541 ttttacgcgc tggcaatggc ccatctcgcc gccgctgccg gttatgccgg gatcgaacat
     3601 cactgcctgc atcagcatca ggccccggca gcggaaacag cctgctgctg tgcggatcag
     3661 ccagaagcag cggcatcctc ctgcccggcg ccgatctcag cgaaagtgct gtcatcatcc
     3721 gccccggctg cctcatccgc ggatgccgag tccccctgct ccgcagcaga gatggcaccc
     3781 gctttaccag ctgcagagga atccaccccc gtactggtcc aaccagactg ctgcgagctg
     3841 gtcagcctct accatcgcgt tgaggagagc gccccgccgg ctttcacggc gctcctccag
     3901 gccccggctg ctcccatcgc cctgcttccc cccaaccggt cgtccgccgc gggcattgtt
     3961 tgctgcactg cgaccgttca tactgccgag cgcctcaatc ttcccctgct catttgatcc
     4021 ctgcgtacat ctctcaccaa tgattttggg cgttccggat ctccggagcg ccccattgaa
     4081 tccttgtatc cagttctcac cagagaggta cgccatgaat aaactcatcc tgtttctctt
     4141 cttctgggcc ggcgctgccg cacttgaagc ccagcccagg gatacctgga cctgcggcca
     4201 tgtctacggc atcgagctgc acggcgccca tcaggacacc atccccctca ttcacgccaa
     4261 tgtgatgtgg gccggcacca ccctcggcac cgtctccgat gacgagggct ttttcaaact
     4321 cgaccttgca ccggccagta aaaaactcgt cgccggatat gtcggttatg aaagcgatac
     4381 cctcgtcgtg gagccgggga tgcacggcct cgccttttac ctcgagccgg acgatcacca
     4441 cgaggagatg accatcgagg gtaaaaagcc ccataccctt catctccacg aggctgaggt
     4501 gaacacccag agcatcaccc ggcaggggct gcgtacgttg gcctgctgca atctggccga
     4561 aagttttgag aacagcctct cggtcgacgt cgagcagagc gacgccgtca gcggcgccaa
     4621 gcgcatcaag atgctcggcc tcgccggttt ttacacccag atcctggtcg aaaagaaccc
     4681 cctcatgcgg ggcctgatca gccccttcgg cctggagtac atccccggct tctggatcga
     4741 cgccatcgac atttccaagg gcaccgcctc ggtggcgacc ggctatgaat cgatcaccgg
     4801 ccagatcaat gtcgagctcg ccaaacctga agacagccgt ccgctcgccc tcaacgccta
     4861 ccagaacagc atggggcgct ctgagttgac cctctcagcg gctcgcaagg tgaccccgaa
     4921 actctccacc atgctccttt cctacggcag ctacaaccgc cagcgctggg acgaggatgg
     4981 cgacaccttc ctggatatgc ccctggtctc tcacatcagc ctgatgaacc gctggaaata
     5041 ccacagcagc agtggcgacg ggcagatcgg tttcaagatc ctgcgcgaca cccgtgacgg
     5101 cggccagacc ggcttcgatt tcggccgccc gcgcgccgcc aacagcggct acggctcccg
     5161 caatcgcatc ggacgctacg agttcttcgc caaggccggc accgccttcg gcggcggccg
     5221 cagcatcggc ctcatcctct ccggcttcca ccacgaccag gactccttct ggggcgccaa
     5281 aggctacaac ggcctggaac aaagcctcta tgccaatctg atcttcgaga aagaggcagc
     5341 acaccaccgc ttctcctccg ggctctcctt cctcctcgac gagcgcgacg agacttacca
     5401 ggaacagctc ttcaaaaccc gcgagcgcgt gcccggcctt tttgcagaat acacctggaa
     5461 gcccaacgac cggctgagcg cgatggccgg atttcgctac gataaccaca acctctatgg
     5521 aggcttttac accccccgcc tccatctcaa gtatcaggct gacgccctca ccgcactgcg
     5581 cctttcggcg ggcaagggct atcgcgtccc ccatctcttc atggacaacc ccgccatgct
     5641 cgccagctcg cgcgaggtga tctttgctga atcccttgag gccgaggagg cgtggaacgc
     5701 cggcatgcag ctcacccgcg agttctcgct cggccaggac cgccccggca ccctggccct
     5761 cgatttctac cgcaccgagt tcctccagca gaccgtggtc gatctcgacc gcgatccgcg
     5821 caccatcctc ctctacaacc tcgacggccg ctccttctcc aacagcgccc agattgaggt
     5881 gaccgccacg gcggcgcggg gcctcgacct gaccgcagcc tggcgcttca atgacgtgcg
     5941 catgacctat gcgggagagc tcaaggaggt gccgctcaat ccccgccaca agggggtgct
     6001 ggtgctctcc tggagcccga cggggcgcaa atggcagttc gacgtcacca cgcagtacaa
     6061 cggcgccacg cggctgccgg agacggctct caatcccgag ccttatcgga tgggcagcca
     6121 ttcgcccgcc tatgggcagc tctttgccca ggtgacgcgc aaatttggca agatggatct
     6181 ctatctgggc ggtgagaatc tgactggctt ccggcaggag cggccgattt tggcctggga
     6241 tgatcctttc agcccctggt tcgactcgtc gctggtctgg gggccgactg ccggacgccg
     6301 gttctatgta ggattgaggg tgaactgatt gcgggctgcg cgcctcactt tttccgttcg
     6361 accacgtgca ggaccttgcc ctccttgtcc atgatataga cgcggagatc ggcggcggag
     6421 acggagcaga ccgaaaagcc ggaggcggcg gcgcagaacc gggttccttc gatgggcgag
     6481 accttgcgga attccgaggc ggagctgttg acgatgtaat cgaccggcga gtcggcacgg
     6541 cggatatgct gatggctgtg gatgtggccg cagatataga tgtcgacctt gtgcttgcgc
     6601 agcaggggat cgaggcgctg ctgcatgtcg cgcctctcgt cctcggtctt ggtggtctgg
     6661 gcatagacgg gatggtgccc catcaccatc gtccaggtgg cggtgttgac cgtcagcact
     6721 gaatcgatcc aggtgagctg ccgctgcaaa tcctgcttcc cggcatcggg atactcttcg
     6781 tggttgttga ggtatttgtc gatcagcggg ggggtgtcga taaagacaag acggagggtg
     6841 gcgccgctgt cgtcgaccgc aaagaccttg gtatagtagc ggccttccat cgcccagcgg
     6901 cggctcacct tgcggtaatc taggacagcc tgggtgttgc cgcgatattc atgattgccg
     6961 atgaccggat accattcgca catcagctcg ggatgattgt agatcagttc atagttggtc
     7021 atccagaggg ggtcctggac actggccaca ccgttgaaat ggtgggcatc gccaagcagg
     7081 gcgacgaact cgacatcaac cttctccccc acttctccca tttttgcagc gaccggtttc
     7141 tgatcataat agccgttgcg gcccagatca ttggcgatga gaaaattgaa acggtcatcc
     7201 acctcctgtg caaggtcgcg ctcctgggcg ggggcgaggg atgcgatgat cagaaacagt
     7261 gccagcagtg cttttttcat accactctcc agtgtaaggt tgtgttgcac tcttgccaga
     7321 ggcgccactt cagattcgcc gcctggcccc cctcaccatg acttacattg cagttatatt
     7381 tccagccaga ccagccgcac tttttccagc aagcagggct ttttctccaa aagcaagttt
     7441 acaaacagcg gagatttgtt ttgcctggca tgaggctgag aactacatga accaccgctt
     7501 ctggccatgc accacatccc cgaagccgcg catgaaggcc agctcctctt cctgcagggg
     7561 gccctggcgc accgccgcga tattctcccg cagctcgcgt tcgctgcgcg gggcggtcag
     7621 tacgcagtgg acatgggggt tggagaggac gaagcggtag cactcaccgg ccgtcggcac
     7681 gcgggcgtct gcaggcaggc tccgcgggcg gcgtacgagg tagctccagc gcgtggcggt
     7741 gtagccgagc acccctggat tgtgcgccgc gaggtgggga aagatatcct gctcggcgcc
     7801 gcgatgggcc gcattgtagc gcagcatcag ggcatccatc tcaccgctcg cggccagctc
     7861 gccgagaaag aggcggttgt ggcaggagag gccgacggcg cgcaccttgc cctcctcttt
     7921 cagcttgagc agctcgtcgc gcaccttttg cggaaattcc gccggcttca tcacccccag
     7981 aaaaagaaag atatcgatgt aatcggtttt gaactgacgc aggcgctttt cgcaggtctt
     8041 gcggatgttc tgcgcccacc agatataatt gtaggctccg gtggcgagga ccagctgctc
     8101 gcgccgcgca ggcaggatct ggcgcagccc gccggtcatc tgggtgtcga tgccgaaggc
     8161 gaaaaagagg ttgatgcctt cgtccgaggc gagctgcacc gcatgcttgc cagggcggta
     8221 agaggacgag agtccaagtg gagagaccat gatgccggtg ctgccgaaag gacgaagggg
     8281 tgagaatcca gtcataatta ttcttttctc cagggacagg ttgtggggtt atctgaccag
     8341 caccatcttg ccggaggaga ttcccgtctc gctcacgaga taatagggat agaggcccgc
     8401 tggcagcgca ccggcgtcaa agcggatctg gtggctgcct gcgtcctgca cctcatccac
     8461 cgggacctca ataacccggc cgcgcaggtc gtggatggtc aacgtgacat gccccctgcg
     8521 cagcagatgg tagtgaataa ccgtcgccgc gttgaaggga ttgggatagt tttgcaacag
     8581 ctccgcgtgc accggccctg ctgcggagat gtggaccccg agcgggtcca cgccgaaggt
     8641 ggtcatgacc ccggcatggt cggagggata attgccgcgg aaagaatcct tcatgaccac
     8701 ggcggaagcc aggggcttga tcttgctgct tttgtaaaag acgtaatcga tccgggcgtt
     8761 gggtgcatcg caagggaccg tatatcccgg cagggagccg ttcacctttc tccagctgtc
     8821 gaccagtgct gccgagagag tggtgatgga ttccg
//
LOCUS       DAOPWU010000128         8625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8185_length_8625_cov_4.890782, whole genome shotgun sequence.
ACCESSION   DAOPWU010000128 DAOPWU010000000
VERSION     DAOPWU010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8625
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8185_length_8625_cov_4.890782"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="PKM23_08010"
     CDS             complement(<1..1404)
                     /locus_tag="PKM23_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNY91458.1"
                     /translation="MKTLRWTLLVIALMASTALTQVLTHELHSNWSFSPDGDQTSWLD
                     AEVPGCVHTDLMRHHLIPDPHYRINEKFMQWIGERDWLYRTGFIPAEELLAREKIELV
                     CEGLDTYARVFLNGHEILHADNMFRIWRADVKPWLVRGPNQLEIRFRSVFSENLPKWE
                     AAPFRLTAYPNNDQADVKINMYSRKAGFHYGWDWGPRLITCGIWRPIRLEAWDGCRLE
                     DLQFRQRSLSDRKAELSAEFTLTAERAQQAVLSVEHEGRRLVRRAVSLKPGRSTVILD
                     FIIKNPKRWWSNGLGEPFLYTLTGRAETASAGQSQPAARKGVQEKSRRIGLRTLRIVR
                     DKDQWGRSFYVELNGTPVFMKGANYIPQDNFQNRVTRARYEHILGSAKAAKMNMLRIW
                     GGGIWEEDLFYDLCDEYGILVWQDLMFACAMYPADESFLDNVRAEVRDNIRRLRNHPS
                     IALWCGNNENQISWYAWG"
     gene            complement(1416..2603)
                     /locus_tag="PKM23_08015"
     CDS             complement(1416..2603)
                     /locus_tag="PKM23_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HNY91459.1"
                     /translation="MQVESFAERLARLTREHEAYVTRRNMPLPWHNGIYQRYTHPVLT
                     AGHAPLFWRYDLDPQRNPFLMERMGINAAFNAGAIELDGKILVMARVEGWDRKSFFAV
                     AESPNGIDNFRFRDFPVVMPETEDPDINVYDMRLVRHEDGWIYGIFCTERKDPNAPPG
                     DLSSAVAQAGIARTRDLVKWERLPDLKSRSPQQRNVVLHPEFIDGQYAFYTRPQDDFI
                     SAGSGGGIGWALCKDVAHAVIEEEAIIDQRLYHTIKESKNGLGPAPIKTPQGWLQLAH
                     GVRGCAAGLRYVLYLFMTDLDHPEKPIYTPGGYFIAPQAEERVGDVSNVVFSNGWVAR
                     DNGDILIYYASSDTRLHVATTTVERLVDYVRKTPPDPLRSAACVAQRNELINRNLALL
                     RRG"
     gene            complement(2650..3852)
                     /locus_tag="PKM23_08020"
     CDS             complement(2650..3852)
                     /locus_tag="PKM23_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNY91460.1"
                     /translation="MASETCRQLAARTERELLDNILPFWLEQAPDREQGGFWARVSPD
                     LQVRERSPRGLILTTRILWTFAAAFHQYKNPAYLEMARKAYEEVCSAFIDPRYGGVFW
                     MLDAERRPIDDSKKIYGQAFAIYSLVEYHAASGDAGALEQALAIYELIEKHNYDPLHT
                     GYLESANRDWSPTEELRLSAIDMNEKKSMNTHLHLLEAYTRLYREWRDDGLRERLVSL
                     VNNFFDHIIDSRSHHLILFFDESWQPKSGKVSYGHDIETSWLLDEATQLLTDPILARK
                     CQKVSVAMAEAVLAEGVAPDGGLIYERDESGRPDPDVHWWVQAESVVGFANAYQNSGR
                     PEFLAAAVRAWDFIAEHLIDRIHGEWFYKVDGERRVDRQLHKISEWKCPYHNSRTCLE
                     LARRVKKC"
     gene            complement(3842..5695)
                     /locus_tag="PKM23_08025"
     CDS             complement(3842..5695)
                     /locus_tag="PKM23_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNY91461.1"
                     /translation="MMILPKRYAAVLLAALFLAGGTLSAAPAPSTFVHFVTVSGDRLM
                     DGPDPLRFISFNIPNLHCIEDYMLFEETNVWRMPDAFEIRDALESIRAMGGTVTRMYT
                     ITVRREDDTPDIPRYVTGPGQFNEEAFRALDQVMAIANQVGVRVIIPFVDNWPWMGGR
                     AEYAAFRGKKADDFWTDPQIKEDFKQTISYIINRKNSITGTTYRDDKAILAWETGNEL
                     YSPDAWVEEIAAYIKSQDKNHLVLDGVAPNRLRDETLANPHTDIITTHHYEKDPRDMV
                     RNIRASAAKAKGKKPYLIGEFGFLGLTGVEEVLREVESHPGVCGALIWSLRFHSRDGG
                     FYWHSEPGNGGYFYKAYHWPGFASGENYAEREVMQLMRERGFAIQGKSVPILTVPKAP
                     QLLPFDDPAHICWRGSVGASGYDVERSASRGGPWKRIGYNISDAETADRPLFHDRSAA
                     IGSTWYYRVRARNAAGLSSPSNLVGPVTTRHKTLVDECWNFAIAFSRKGHLSLETADS
                     RLFKEDAHRIAGVPGAELVYYIPGPLQTLKFYTFAASAAKSIEVSLSADGEEYTPLQV
                     EARDFFAGAADYEYLRPLLFASSGLPDGIHYVRVAFLGEAQLGRAELIYGK"
     gene            complement(5719..7131)
                     /locus_tag="PKM23_08030"
     CDS             complement(5719..7131)
                     /locus_tag="PKM23_08030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNY91462.1"
                     /translation="MTRCALIALSFGLLLLACGRKDPAVARLGRERITLEEFKTAYLE
                     IIKQPNAFDSRELREQFLDEMIDRRLLAREAHGLGMAADERLAYRVGANRDRNLRDAH
                     FRKVIKPGISYNEADLERTFAWMTEERHIRHLFSPTRVGIDSLYHLLQEGADWFELAR
                     GLFDDPRLAETGGDLGWVAWEQMEYDLGFTAFSLPLMVYSEPVASSYGWHILQVADWR
                     RTPLLPEGQYQLQRNNARAVLERKLGDKAALAYISGMMKEKKIQVYPRSLRAVGQKLG
                     VVLKRQPSTADQLRTEQLSDAEMGEIEASLWEMRKEPLASIDGEILTIGEFIAALNYV
                     PYHAVHRSMKTALDFVLRDRALSAEARRMGLERDDQVRIKTAQFEQNRLQMAIRQRLL
                     AEIRVEERDLQRYFNEHYGKKYPHLGLEETRDLIARDALIAKRAGTMQEHLQKLRSQL
                     RIERNPAPIHAWYEGVLNRR"
     gene            complement(7270..8442)
                     /locus_tag="PKM23_08035"
     CDS             complement(7270..8442)
                     /locus_tag="PKM23_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91463.1"
                     /translation="MQFLDHSMDHRDNDNDGLIDGEDVDELMPTVTSGFVLRNLTQTS
                     LPEDTVMIIQREYSAGEWVEVKNLYEDADSDPRTLTTVLHGMEFNIYNPPEGMINRPE
                     ERIYNGVQWSSNINFTTAYNIRFSIFNLSGFKEGTYYPRQYMVVFYDDIVKKSETLQV
                     PLATSNKTIPLPATDANFRVYDKQTMEELPFALVDQTLTPKKIKRGMFSAKDRIVMFE
                     KLPNDSTLITFNILNNASEDTVFWNTYGRALGAGDTLNVYPDFPFTGNVRYQFVVKGQ
                     KVDNAFAARNLDKIKVVPNPYVVAAAWEPTNPYTSGRGPRSVQFIHLPQKCTIRIFAV
                     DGTLVNTIEHDSEMTDGSEEWDMFTKDRMDIAYGVYIYHIEAPGIGNTTGRMLIIK"
     gene            8425..>8625
                     /locus_tag="PKM23_08040"
     CDS             8425..>8625
                     /locus_tag="PKM23_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91464.1"
                     /translation="MVEELHLVAVAVADLIRLKVAEVDRPRGLAGADLARLFGEAGAA
                     VAERGRLGTTTTTLSPVVMSPEA"
BASE COUNT         1634 a         2470 c         2760 g         1761 t
ORIGIN      
        1 gccccaggca taccaggaga tctggttttc gttgttgccg caccagaggg cgatagaggg
       61 gtggttgcgc aggcggcgga tgttgtcacg aacctcggcg cggacgttgt cgagaaagga
      121 ttcgtcggca ggatacatgg cgcaggcgaa catcaggtcc tgccagacca ggatgccgta
      181 ttcatcgcag aggtcgtaaa agagatcctc ctcccagatg cctccgcccc agatgcgcag
      241 catgttcatc ttggccgcct tggccgaacc gagaatgtgc tcatagcgcg cccgggtgac
      301 ccggttctga aagttatcct gggggatgta attggccccc ttcatgaata cgggcgtgcc
      361 gttgagttcg acgtagaacg agcgccccca ctgatccttg tcgcgcacaa tgcgcagggt
      421 gcgcaatccg atgcgccggc ttttctcctg cacgcctttc cgggctgcag gctggctttg
      481 gccagcggag gcggtttcag cccgcccggt cagggtgtaa agaaagggct ccccgagacc
      541 attgctccac cagcgtttcg ggtttttgat gataaagtcg agtatcaccg tgctgcgccc
      601 gggcttcagg ctcaccgcac gccgcaccaa acgccggccc tcatgctcca ccgaaagcac
      661 cgcctgctga gcgcgctccg ccgtaagggt gaattcagcg ctcagctcgg ccttgcggtc
      721 gctgaggctc ctctggcgga actggagatc ctccagccgg cagccatccc aggcctcgag
      781 gcggatcggc cgccagattc cgcaggtgat cagacgcgga ccccagtccc agccgtagtg
      841 aaaacccgcc ttgcgggagt acatgttgat cttgacgtcg gcctggtcgt tgttgggata
      901 ggcggtcagc ctgaaagggg ccgcctccca tttgggcagg ttttcggaaa agacggaacg
      961 gaagcgaatc tcgagctggt tgggacccct gacaagccag ggtttgacgt ccgctcgcca
     1021 gatacgaaac atattatcgg cgtgcagtat ctcgtggccg ttgaggaaca cacgggcgta
     1081 ggtatcgagc ccttcgcaga ccagctcgat tttctcccgc gcgagcagct cctccgcagg
     1141 tatgaacccg gtgcggtaga gccaatcccg ttcgccgatc cactgcatga atttttcgtt
     1201 gatgcggtaa tggggatcgg gaatgaggtg atggcgcatg agatcggtgt ggacgcaccc
     1261 cggcacttcc gcatccagcc aggaggtctg gtcgccgtcc ggactgaaag accagttgga
     1321 atgaagctcg tgggtcagga cctgtgtcaa agcggtggag gccatcagcg caatcaccag
     1381 gagtgtccat cgcagtgttt tcatatctct ttccatcatc ccctgcgaag cagggcaagg
     1441 ttgcggttga tcagttcgtt gcgctgggcg acgcaggcgg ccgagcgcag gggatcggga
     1501 ggggttttgc gcacataatc gaccaggcgc tccaccgtag tggtggcgac atggagacgg
     1561 gtatcagagg aggcatagta gatcaggatg tcgccattgt cgcgcgccac ccagccgttg
     1621 gaaaagacga cgttggaaac gtcgccgacg cgttcttcgg cctgcggtgc gatgaaatag
     1681 cccccggggg tataaatggg cttttccggg tggtcgaggt cggtcatgaa gagatagagg
     1741 acgtaacgca gtcccgctgc acagccgcgt acgccgtggg ccagctgcag ccagccctga
     1801 ggggttttaa tcggggccgg gcccaggccg ttcttcgact ccttgatggt gtgatagagg
     1861 cgctggtcga tgatggcctc ctcctcgatg accgcgtgtg cgacatcctt gcagagggcc
     1921 cagccgatgc cgccgccgct gccagccgag atgaaatcat cctggggacg ggtgtaaaag
     1981 gcatattggc cgtcaatgaa ctcgggatga agcacgacat tgcgctgctg cggcgagcgg
     2041 ctcttgaggt cggggagccg ctcccatttc accaaatcgc gggtgcgggc gatgccggcc
     2101 tgcgccacag ccgaggagag atcgccggga ggggcattgg ggtccttgcg ctcggtgcag
     2161 aagatgccgt agatccagcc atcctcgtgc ctgaccaggc gcatgtcgta gacgttgatg
     2221 tccggatctt cggtttcggg catcaccacc ggaaagtccc ggaagcggaa attatcgatg
     2281 ccattggggc tctcggcgac agcgaagaag gatttgcggt cccagccctc gacgcgagcc
     2341 atgaccagga tcttcccgtc aagctcgatg gcgccggcgt tgaaggcggc gttgatgccc
     2401 atccgctcca tgagaaaggg attgcgctgg ggatcgaggt cataacgcca gaagaggggg
     2461 gcatggccgg cggtcagcac cggatgggta tagcgctggt agatgccgtt atgccagggc
     2521 agcggcatat tccggcgggt gacataggcc tcgtgttccc gggtcagcct ggccaggcgt
     2581 tcagcaaagg actccacttg catcattttc tcctcgataa tactttcaat ataacgagcg
     2641 gtgttactct cagcattttt tcacccgccg cgccagttcc aggcaggtac ggctgttgtg
     2701 ataagggcat ttccattcgg atatcttgtg cagctgcctg tctacccgcc gttcgccgtc
     2761 caccttgtag aaccactccc cgtggatgcg gtcgatcaga tgctccgcga tgaaatccca
     2821 ggcccggacg gcggcagcga ggaattccgg acgaccgctg ttctggtagg cgtttgcaaa
     2881 tccaaccaca gactccgcct gcacccacca gtgcacatcg gggtcggggc ggccactctc
     2941 gtcccgctca tagatgagcc cgccatccgg ggccactccc tcggcgagca ccgcctcggc
     3001 catggccacc gacaccttct ggcacttgcg cgccaggatc ggatcggtga gaagctgggt
     3061 cgcctcgtca agcagccaac tggtctcaat atcatgtcca tatgagacct tgcccgattt
     3121 aggttgccat gattcatcaa agaaaaggat gaggtgatgg ctgcggctgt cgatgatatg
     3181 gtcgaaaaaa ttattgacga ggctgaccag ccgttcgcgc agcccgtcat cccgccattc
     3241 tcgatagagc cgggtatagg cttcgagcag gtggagatgg gtgttcatcg actttttctc
     3301 gttcatatcg atcgcactga ggcgcagttc ttcagtgggt gaccaatcgc ggttggcgct
     3361 ctcgagatag ccggtatgta gcggatcgta attgtgtttt tcgatcagtt cgtaaatggc
     3421 cagggcctgc tcgagggccc cggcatcgcc ggaggcggca tggtattcga cgaggctgta
     3481 aatggcaaag gcctggccgt agatcttttt actgtcatcg atgggccgcc gttcggcatc
     3541 gagcatccag aagactccgc cgtaccgggg atcgatgaag gccgaacaaa cctcctcata
     3601 ggccttgcgg gccatctcca gataggccgg atttttatac tggtggaaag cggcggcgaa
     3661 ggtccagagg atgcgggtgg tgaggatcaa tccccgcggc gagcgttcgc gcacttgcag
     3721 atccggtgag acgcgcgccc agaatccgcc ctgttcccga tccggcgcct gctcgagcca
     3781 aaaggggaga atattatcca gtagctcccg ctcggttctc gccgccagct gcctgcaggt
     3841 ttcacttgcc atagatcagc tctgcccttc ccaattgggc ctccccgagg aaggcgacgc
     3901 ggacatagtg aatgccgtcc ggcaggccgc tgctcgcaaa cagcagcggg cgcagatact
     3961 cgtaatcggc cgcaccggcg aagaaatccc gtgcctcgac ctgaagcggg gtgtactcct
     4021 ccccgtcggc cgaaaggctc acctcgatac tcttggccgc cgaggcagcg aaggtataga
     4081 atttgagggt ctgcagcgga ccggggatat aatagaccag ctccgccccc ggcaccccgg
     4141 cgatgcggtg ggcatcctcc ttgaagaggc gggaatcggc ggtctcgagg gagagatggc
     4201 ccttccggct gaaagcgatg gcgaagttcc agcactcatc gaccagggtc ttgtgccggg
     4261 tggtgacagg accgacgaga ttggaggggc tggagaggcc ggcagcgttc cgggcgcgca
     4321 cgcgataata ccaggtcgat ccgatcgcag cgctgcggtc atggaagagc ggccggtccg
     4381 cggtctcggc gtcggagatg ttgtagccga tccgcttcca ggggccgccg cgggaggcgg
     4441 agcgctcaac gtcgtaaccc gaggcgccga cggagccccg ccagcaaata tgggccggat
     4501 cgtcgaaggg aagcagctgc ggggcctttg gcaccgtcag gatgggcacc gatttgccct
     4561 ggatggcaaa accccgttcg cgcatcagct gcatcacttc gcgctcggca taattctcac
     4621 ccgacgcgaa acccggccag tggtaggcct tgtagaaata gcccccgttg ccgggctcgg
     4681 aatgccagta aaagccgccg tcgcggctgt ggaagcgcag gctccagatc aacgccccgc
     4741 agacgccggg atgggattcc acctcccgca gcacttcctc aacaccggtg aggccgagga
     4801 aaccgaattc cccaatcagg tagggctttt tgcccttcgc cttggcagcg ctggcgcgga
     4861 tgttgcgcac catatcgcgc ggatccttct cgtaatgatg ggtggtgatg atatcggtat
     4921 gaggattggc gagggtttca tcgcgcagac gattgggagc gactccgtcg aggacgaggt
     4981 ggttcttgtc ctgagattta atataggccg cgatctcctc cacccaggca tcggggctgt
     5041 agagttcgtt gccggtctcc caggccagga tcgccttgtc gtcgcggtag gtcgtgccgg
     5101 tgatggagtt tttgcggttg atgatgtagg agatagtctg cttgaaatcc tccttgatct
     5161 ggggatccgt ccagaaatca tccgcctttt tgccgcggaa ggcggcgtat tccgccctcc
     5221 cccccatcca gggccagttg tcgacgaagg ggatgatcac cctcaccccc acctggttgg
     5281 cgatggccat gacctgatcg agggcgcgga aggcctcctc attgaactgc ccgggtccgg
     5341 tcacgtagcg cgggatgtcc ggggtgtcat cctcccgccg cacggtgatg gtatacatcc
     5401 gcgtcaccgt cccccccatc gccctgatcg actcgagggc gtcgcggatt tcaaaggcat
     5461 ccggcatgcg ccagacattg gtctcttcga agagcatata gtcttcgatg cagtgaagat
     5521 tgggaatatt gaaggagata aagcgcaggg gatcgggacc atccatcagc cgatcgccgg
     5581 aaaccgtgac aaaatggaca aaggttgatg gggccggtgc ggccgaaagt gttcctccgg
     5641 ccagaaacag cgcggcgagc agaacggctg catacctttt aggcaaaatc atcatgatgc
     5701 tctcctgtga agatgagttt atcgcctgtt caacacccct tcgtaccagg catggatcgg
     5761 cgccggattt ctctcgatgc ggagctggct gcgcagcttt tgcaggtgct cctgcatcgt
     5821 gccagcccgt ttcgcgatga gagcgtcccg ggcgatgaga tcgcgcgtct cctccagtcc
     5881 gagatgggga tacttttttc catagtgctc attgaaatag cgctgcaggt cccgctcctc
     5941 caccctgatc tccgcgagca ggcgctggcg tatggccatc tgcagccggt tttgctcgaa
     6001 ctgggcggtc ttgatgcgga cctgatcatc gcgttcgagt cccatccgcc gcgcctccgc
     6061 gctgagggca cggtcgcgca ggacaaagtc gagggccgtc ttcatgctcc tgtggacggc
     6121 gtggtagggc acgtaattga gcgcggcgat gaattcgccg atggtgagaa tctcgccatc
     6181 aatgctggcg aggggctcct tgcgcatctc ccagagcgag gcttcgatct cccccatctc
     6241 cgcatcgctg agttgttcag tgcgaagttg atcggcggtg gagggctggc gtttgagcac
     6301 cacgccgagc ttctggccga cggcgcgcag gctgcgcgga tagacctgga tctttttctc
     6361 cttcatcatc ccgctgatat aggcgagggc cgccttgtcg ccgagcttgc gttcgagcac
     6421 cgcccgggca ttattgcgct ggagctggta ctggccctca gggagcaggg gcgtccggcg
     6481 ccagtccgcc acctggagga tgtgccagcc atagctggag gcgaccggct cggagtaaac
     6541 catgaggggg aggctgaagg cggtgaagcc caggtcgtac tccatctgct cccaggcgac
     6601 ccagccgagg tcgccgccgg tttcagcgag gcgcgggtcg tcaaagaggc cgcgcgccag
     6661 ttcgaaccag tcggcaccct cctgcaggag gtggtaaagg gaatcgatgc ccacccgggt
     6721 cggactgaag agatggcgga tatggcgctc ctcggtcatc caggcgaagg tgcgctcgag
     6781 atcggcctca ttgtagctga tccccggctt gatcaccttg cggaaatggg cgtcgcgcag
     6841 attccggtcg cgattggcgc cgacgcgata ggccagccgt tcgtcagcgg ccatgcccag
     6901 cccgtgcgcc tcacgcgcaa gcagccgccg gtcgatcatc tcgtcgagga actgttcgcg
     6961 cagctcccgg gaatcaaagg cgttgggctg cttgatgatt tccagatagg cggtcttgaa
     7021 ctcctcgagg gtgatacgct ccctgccgag acgcgcaacg gcgggatcct tcctgccgca
     7081 ggcgaggagg agcagaccga aagagagagc gataagtgca caccttgtca ataaggactc
     7141 ccgaagcgga atcgcgcaaa gacgcagagg cgcagagaaa actgctcttc tctgcgtact
     7201 ctgcctccca gcggggaata atggttttgt ttggcggcag tgaaaaaatc catcccctgc
     7261 caaaactgat tatttaatga tcagcatccg gcccgtggtg ttgccgatgc ccggggcctc
     7321 gatgtggtag atgtagacgc cgtatgcaat atccatgcgg tccttggtga acatgtccca
     7381 ctcctcggag ccgtcggtca tctccgaatc atgttcgatg gtattgacca gggtgccgtc
     7441 gaccgcgaag atacggatgg tgcatttctg cggcaaatgg atgaactgga cggaacgggg
     7501 ccctcgcccg ctggtatagg ggttggtggg ctcccaggca gcggcgacga catagggatt
     7561 gggcacgacc ttgatcttgt cgagatttct ggctgcaaag gcgttgtcca ccttttgtcc
     7621 cttgacgacg aactgatagc gcacgttgcc ggtaaaggga aagtcgggat agacattcag
     7681 ggtatccccg gcgccgaggg cgcggccgta ggtattccag aaaaccgtgt cctccgaggc
     7741 gttattgagg atgttgaaag tgatcagggt cgaatcgttg ggcagctttt cgaacatgac
     7801 gatccggtcc ttggccgaga acattcccct cttgattttc tttggggtca gggtctggtc
     7861 gaccagggcg aagggcagtt cctccatggt ctgcttgtca tagacgcgga agttggcgtc
     7921 tgtcgcaggc agggggatgg tcttgttgct ggtcgccagg ggcacctgga gggtttcgga
     7981 tttcttgacg atgtcatcat agaagacgac catgtactgg cggggatagt aggtcccctc
     8041 cttgaatccg ctcagattga agatggagaa gcggatattg taggcggtgg tgaaattgat
     8101 gttcgaactc cactgtacgc cgttgtagat gcgctcctcc ggccggttga tcatgccctc
     8161 agggggattg tagatgttga attccatgcc gtggaggacg gtcgtgaggg tgcgggggtc
     8221 gctgtcggca tcctcgtaga ggtttttcac ttcgacccat tccccggcac tgtactcccg
     8281 ctggatgatc atgaccgtat cctcaggcag ggaggtttgg gtcaggttgc ggaggacaaa
     8341 tccggaggtc actgtgggca tcagttcatc gacatcctct ccgtcgataa ggccgtcgtt
     8401 gtcgttgtcc cggtggtcca tcgaatggtc gaggaactgc acctcgtagc ggtcgccgtc
     8461 gcggatctca tccggcttaa ggtagcggag gttgaccgcc cccgaggtct tgccggtgcc
     8521 gatctggcgc ggctgttcgg agaggccggg gccgctgtag ccgagcgagg gcgcctgggc
     8581 accaccacga cgacattatc gccggtggta atgtctcccg aggcg
//
LOCUS       DAOPWU010000129         8622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8190_length_8622_cov_4.752889, whole genome shotgun sequence.
ACCESSION   DAOPWU010000129 DAOPWU010000000
VERSION     DAOPWU010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8190_length_8622_cov_4.752889"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKM23_08045"
     CDS             complement(<1..190)
                     /locus_tag="PKM23_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91465.1"
                     /translation="MSDSKIPTPDQLRRDVNEFLKEKYGDRVVIPPEPDLNGQTPPPE
                     GEKKDFTSPVHFNLKPSEL"
     gene            complement(367..1224)
                     /gene="nadC"
                     /locus_tag="PKM23_08050"
     CDS             complement(367..1224)
                     /gene="nadC"
                     /locus_tag="PKM23_08050"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNY91466.1"
                     /translation="MDEEIRAAAAELIRMALTEDLADRGDITSAAIADAAGPCRGIFL
                     AKAEGTIAGLDIARMTLAAVDPELTGHPFIRDGDRVRKGDILMEVKGEAGSLLVAERT
                     ALNFLGRLSGIATLTRRFTDAVAGTRARILDTRKTTPGWRLLEKYAVRCGGGVNHRMG
                     LHDMFLIKDNHVIASGGITPAVERCRAYAQRMGFQAEIEVETRTLAEVEEAVALRVDR
                     IMLDNMSLDLMHEAVERVSGRIPLEASGNVTLESVAAIARTGVDFISSGSLTHSARVL
                     DISLDLLQG"
     gene            complement(1230..4919)
                     /locus_tag="PKM23_08055"
     CDS             complement(1230..4919)
                     /locus_tag="PKM23_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY91467.1"
                     /translation="MRILRYHLVLSLLMLLLFPTLLPAQEGLLQEEKDFAFATQLADK
                     ELYDLAAQQFVKFADAWPTSPRAPEALFNAAENYEAIASFQRAADTYLRLVMSYPEAA
                     NADKAFFNRGKLLAVLGDPLNAALTLERIRIFTPNSELIPLAMVSAAEQFRSAGEAQK
                     AYSAAAAAIAQYPDSPVRPRAFFLLARLQHDAGKPALALTELNRIGAARVENTLATQI
                     ALLRARVLSELGRYSKSDSVLEVLVNSDTANDSIGAAACLLAQSLYSRGLYSKVIQTT
                     ERSLQRRIRPHERHQLQLYQGDSWASLGDPARALSSLSRIPHDSLKAQEAAKLAFRRG
                     MLQKRLGEPALALPWFSQILAHADSLKDITALQRLALQEQTALLLELNNPGEALRSLR
                     LLFEELPPLRDSILMQRASIVRTVLKNPVSARQDYALLSTFYPASPLADDAALGLARC
                     HDEAGDRNVAIMAYQEFLALYPASEEGSEVEKRLTWLRLYQPAGSPERDLWISRALLA
                     GGDSGSALAWAARRIEEDHDYQSGLTILRHILASRREGEVDESGLFYLAGLAHARLAE
                     KYALEGDLTSAGLHADSLQEVNRFLTSRGAADPGSRTINGLAQLASWKRIGRPLIRAG
                     MVDTLLARIQPSDSLAATLKLEQARIWYRFAADSSAHWYSRAAAACTAIAAAYPQPRI
                     RLENALLHSRILSAIQLPDSAIQTLRSALAGGTISAQAAEASLELAGLLEKSNRAGEA
                     AALYEDWLQTYGYAGRADSIRSRLCQIYFRQRQFERARQCMSRFDDGSALQDLSPYLD
                     RKRNETLLWLSAQAWLLQHDLPAAIQAFKEYIRINPEGAHRSEALLELANLYTLSGNF
                     QAAAGHLEELIAASPQDSLALVALMRTADLLYDQHEFREAALRYARIKNAAGGAVQRQ
                     AAMNEVLCEYKQQNLARARQLADAFKKSFKERKAEALFLYEDGMISLANRDFVAAENQ
                     FKELTSKYRDLPEAADGEMGLARLYAALNKTDEALKILTNIPNRYTEPRIVALSYINL
                     GEFYYENRQLENCMAAGRKALEFAPKGAEQQRAIALLITVFDDLRLWDNAVVLLRQYI
                     QDYPEAEDLFNRKMQLGVFLINLKEYERGVDQLKSLLPQADAENEAEIQYWIARAYHE
                     RGDMNQAIIEYLKVKYVCRPSKLPWGTTALYEAGQTYAKLGNLVSARSLFEQIVREMG
                     VGDQFGRVANERIREIDAALARQKG"
     gene            complement(4916..7138)
                     /locus_tag="PKM23_08060"
     CDS             complement(4916..7138)
                     /locus_tag="PKM23_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNY91468.1"
                     /translation="MTRASEKKGTSPEWLTADLNDAQSQAVAKTDGPVLILAGAGSGK
                     TRVLTYRIAWLLANGLAEPREILAMTFTNKAAGEMRERVRKLVPEMLSGMWIGTFHSL
                     FARLLRREAERINYASNFAIYDDDDQHSLIKTVLNDLKIPVNDFPPKMISYKISSAKN
                     AFILPEQFARMAATRQDQIIATIYSTYQTRLRENNAMDFDDLLIKPIELFQLYPLVLE
                     YYQERFRYILVDEYQDTNHAQYVALKMLAARHRNICVVGDDDQSIYRWRGAEIRNILD
                     FENDYPDCAKFRLEQNYRSTRNILAVAHSVVRNNRQRHEKKLWTEKPEGDLITLLEVE
                     DDLEEARTVVNKLALEMRANHMDFQDIAVLYRTNAQSRVLEEALRRENIPYIIVGGVK
                     FYERKEVKDVIAYLRLLSNPADTISLRRIINYPARGIGETTLGRIEAYAQEKKISIFA
                     ATGQVQQIPGIAARTAEQVAAFHGLLNKYTALREEISPAELAASLVNEVGILRDFKQE
                     GTIESLSRAENVRELLLAIAEFTSSAGGEATLDGFLENVALTSDLDSWDDRANAVTLM
                     TLHSAKGLEFPVVFITGLEEGLFPISRSLADPPALEEERRLFYVGATRAKVKLYLSWA
                     RNRRKFGETSASIASRFLREVEPALVQVESSYRAAPIRRQRSSFPRREADPMPRYEDE
                     SQEVLEYRVGMRVHHPTFGKGTIMRLEPSSASSLKLRVLFDQAGEKRLVLPYAKLEIL
                     "
     gene            complement(7140..8186)
                     /gene="mltG"
                     /locus_tag="PKM23_08065"
     CDS             complement(7140..8186)
                     /gene="mltG"
                     /locus_tag="PKM23_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNY91469.1"
                     /translation="MTNKVFHFSLRRAAVALLVLLLLAAAIPLVSILYWPLGQPPQGQ
                     AADIRIKFGTSPDSIAVMMERAGIVGSSSRFLLALKLLNKGGALRAGHFRIPYPLSNK
                     RAIQALTEGPQILTKVTIPEGLTVRRIAAIFRREMAMDSVRFVQLTRDSSFIARFKIN
                     TADLEGYLYPETYFFPYGSTEAQVITPLVQQFKKMVWDTLYQRSQEMGFTTGEIVTLA
                     SIIQAEAKLSREMPTISSVYHNRLRLGIPLQADPTIQFLLPNGPRRLLYRDLEIDSPY
                     NTYLYRGLPPGPIGNPGREAILAALHPEATDYLFFVADTRGGHTFSANFRGHLKAKSV
                     LDRERKRLSQANRK"
     gene            complement(8244..>8622)
                     /locus_tag="PKM23_08070"
     CDS             complement(8244..>8622)
                     /locus_tag="PKM23_08070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNY91470.1"
                     /translation="ALVLTTATDYIMLSMGEERSFHASLMLALGAGVYEEIVFRLFLY
                     GGMALVLTHLTRLPLTIIYLVCAALSSAVFSAAHYMGHEAFAWYTAVFRFLAGLLFCL
                     IYHFRGLGIAGWSHALYDLFLIL"
BASE COUNT         1639 a         2320 c         2976 g         1687 t
ORIGIN      
        1 cgagttcgga gggcttgaga ttgaaatgga ccggactggt gaaatccttt ttctcgccct
       61 cggggggcgg agtctggcca ttcagatcgg gctcgggggg aatgaccacc cggtcgccgt
      121 atttttcctt gagaaactcg ttgacatccc ggcgcagttg atccggggtg gggattttcg
      181 aatctgacat aatgctttcc tttcagagcg ccttgttgcg ccgctgaagt ttaacatcga
      241 ctggaaagtc tttgttcctg cctgctgcag agctcagagc gccatgccga atcttgcacc
      301 ccgtacagaa aggcggtggt gcctcgatgc cgcgcgccgg cggcacatcc tgccttccca
      361 gctgcatcac ccctgcagca ggtcgaggct gatatcgagc acgcgggctg agtgggtgag
      421 gctgccactg ctgatgaaat cgacgccggt gcgggcgatg gcggcgacgc tctcgagggt
      481 gacatttccc gaggcctcga ggggaattct gcctgagacc cgttcaaccg cctcgtgcat
      541 gagatccagg ctcatgttgt cgagcatgat ccggtccact ctgagggcga ctgcctcctc
      601 cacttctgcc agggtgcggg tttcaacctc gatttcggcc tgaaagccca tacgttgagc
      661 ataggcccgg cagcgttcca ctgccggagt aataccgccg gaggcgatga catggttgtc
      721 cttgatgagg aacatgtcat ggagacccat gcggtggttg acgccgccac cgcagcgcac
      781 ggcatatttt tcaagcagcc gccagcccgg agtcgtcttg cgggtatcga ggatgcgtgc
      841 gcgggtgccg gcgacagcgt cggtgaagcg gcgggtcagg gtagcgatgc cggagaggcg
      901 gccgagaaaa ttgagggcgg tccgttccgc caccagcagg ctgccggcct cccccttgac
      961 ctccatgagg atgtcgccct tgcggacgcg gtcgccgtcc ctgatgaagg ggtgtccggt
     1021 gagctcggga tcgacggcag cgagggtcat gcgggcgatg tcgaggccgg caatagtccc
     1081 ctccgccttg gcaaggaaga tgccacggca aggcccggcg gcgtcggcga tggcagcgct
     1141 ggtgatgtcg ccgcgatcgg ccagatcctc ggtcagggcc atgcggatga gctccgcagc
     1201 tgccgctctg atctcttcat ccatgtatat catcctttct gtctggcgag ggcggcatcg
     1261 atttcacgga tgcgttcgtt ggcaacgcgg ccgaactggt cgccaacacc catctcgcgc
     1321 actatctgct caaacaggga gcgggcactc acgagattgc cgagcttggc ataagtctgg
     1381 ccggcctcat agagggcggt agtgccccag gggagcttgc tcggcctgca gacgtacttg
     1441 accttgagat actcgatgat ggcctggttc atgtctcccc gttcgtggta agcccgggcg
     1501 atccagtact gaatttccgc ttcgttttcg gcgtcggcct ggggcagcag gctcttgagc
     1561 tggtcgacgc cgcgttcata ctccttgagg ttgatcagaa atacacccag ctgcattttg
     1621 cggttgaaga ggtcctccgc ctcgggatag tcctggatat actggcggag gagtacgacg
     1681 gcattgtccc acagccgcag gtcatcgaac accgtgatca ggagggcgat ggcgcgctgt
     1741 tgttcggcac ctttgggggc gaattcgagg gccttgcgtc cagcggccat gcagttctcg
     1801 agctggcggt tttcgtaata gaattcaccc agattgatgt aggagagggc gacgatgcgc
     1861 ggttcggtgt agcggttggg gatgttggtg aggatcttga gggcttcatc ggttttgttg
     1921 agggctgcat aaagcctggc cagccccatc tcgccatcgg cggcctctgg cagatcccgg
     1981 tatttgctgg tgagttcctt gaactggttc tcggcggcga cgaaatcacg gttcgccaaa
     2041 gagatcatgc cgtcttcata gaggaagagg gcttcggcct tgcgctcctt gaaactcttt
     2101 ttaaaggcat cggcaagctg ccgggcacga gccaggttct gctgtttgta ctcgcacagg
     2161 acctcattca tcgcggcctg gcgctgcacc gcgcctcctg ccgcgttctt gatgcgggcg
     2221 tagcgcaggg cggcctccct gaattcatgc tgatcataga ggaggtcggc ggtgcgcatc
     2281 agggccacaa gggcgaggga atcctggggc gaggcggcga tgagttcctc cagatgccct
     2341 gcagcggcct ggaaattgcc agacagggtg taaagattgg ccagctcgag cagggcctcg
     2401 ctgcggtggg ctccctccgg attgatgcgg atgtactcct tgaaggcctg gatggcggca
     2461 ggaagatcgt gctgcagaag ccaggcctgg gcggagagcc agagcagggt ctcgttgcgt
     2521 ttacggtcca gatagggtga aagatcctgc agggcgctgc cgtcatcgaa gcggctcatg
     2581 cactgccgtg cgcgctcgaa ctgacgctgt ctgaaataga tctggcagag gcggctgcga
     2641 atcgagtcag cgcggccggc ataaccgtag gtttggagcc aatcttcata gagggcggcg
     2701 gcttccccgg cacggttcga cttttcaagc aaaccggcga gctcgagcga cgcttcggcc
     2761 gcctgggcgg agatggtgcc gccggcgagg gcactgcgaa gagtctggat ggctgagtcg
     2821 ggcagctgga tggcggagag aatcctgctg tggagcaggg cgttctcgag gcggatccgg
     2881 ggctgaggat aggcggcggc gatagcggtg caggctgcgg cggcacgcga gtaccagtgg
     2941 gcgctgctgt cggctgcaaa acggtaccag atcctcgcct gttcgagttt gagggtggcg
     3001 gcgaggctgt cggaaggctg gatccgggcg agcagggtat cgaccatgcc ggcgcggatc
     3061 aggggccgtc cgatgcgctt ccagctcgcg agctgggcga ggccgttgat ggtgcggctg
     3121 cccggatcgg cggcgccgcg gctggtcagg aagcggttga cctcctgcaa ggaatcggca
     3181 tgcagtccgg cgctggtgag atcgccctcc agggcgtact tttcagccag acgggcatga
     3241 gcaagcccgg cgaggtaaaa gaggccgctc tcatccacct ccccctccct ccggctggcg
     3301 aggatgtggc ggaggatggt cagtccagac tggtagtcat gatcttcctc gatacgtcgg
     3361 gccgcccagg caagcgcaga tccggaatcc cctccggcca ggagggcgcg tgaaatccag
     3421 aggtcacgtt caggcgaacc cgctggctgg tagaggcgca gccaggtcaa ccgtttttcc
     3481 acttcgctgc cctcctccga ggcgggatag agggcgagaa actcctgata ggccatgatc
     3541 gccacattcc ggtcgcccgc ttcgtcatgg cagcgggcca gacccagcgc cgcatcgtca
     3601 gccaggggac tggcgggata aaaagtggag agcagggcat agtcctgccg cgcgctgacg
     3661 ggattcttta ggacagtccg cacgatggag gcgcgctgca tgaggatgga atcacggagg
     3721 gggggcagct cttcgaaaag caaacgcagg ctgcgcaggg cttcgccggg attgttcagc
     3781 tcaaggagca gggcagtctg ctcctgcagg gccaggcgct gcagggcggt gatgtctttc
     3841 agactgtcgg catgggcaag gatctggctg aaccagggca gtgccaaggc gggctcaccg
     3901 aggcgctttt gcagcatgcc gcggcgaaag gcaagcttgg ccgcctcctg ggccttcagg
     3961 gagtcgtggg ggatccggct taaagagctc agggccctgg cgggatcacc aagggaggcc
     4021 cagctgtctc cctgatagag ctgcagctga tggcgttcgt gcgggcggat cctgcgctgc
     4081 agggagcgtt cggtagtctg gatcactttc gaatagaggc cgcggctgta aagggattgg
     4141 gcgagcagac aggctgccgc accaatggaa tcgttggccg tatcgctatt gaccagcacc
     4201 tccagaacgc tgtcactttt cgaatagcgg ccgagttcac ttagaacccg ggcgcggagc
     4261 agggcgatct gggtggccag ggtgttctcg acacgcgctg caccaatgcg gttgagttcg
     4321 gtcagggcga gggcgggttt gccggcgtcg tgctgcagcc gggcgaggag aaaaaaggcg
     4381 cgcggccgca cgggcgagtc gggatactgg gcgatcgcag cagcggccgc gctgtaggcc
     4441 ttttgggctt cgcctgcgct gcggaactgc tccgcagcgg agaccatggc aagagggatc
     4501 agttcgctgt tgggggtgaa gatgcgaatc cgctcgaggg tgagagcagc attgagggga
     4561 tctcccagca ccgcgagcag cttgccgcgg ttgaagaagg ccttgtcggc gttggccgcc
     4621 tcaggatagc tcatgaccag acgcaaatag gtatcggcgg cccgctgaaa ggatgcgatg
     4681 gcttcataat tctcggccgc attgaagagg gcctcggggg cgcgagggct ggtcggccag
     4741 gcatcggcga acttgacaaa ctgctgtgca gcgaggtcgt agagttcctt gtccgcgagc
     4801 tgcgtggcaa aagcaaagtc cttctcctcc tgaagcagcc cctcctgcgc cggcagaagc
     4861 gtggggaaca ggagcagcat gagcagtgac aagaccagat gatatcgcaa tatcctcata
     4921 atatctccag ttttgcataa ggcaggacca accgtttctc tcccgcctga tcaaaaagaa
     4981 cgcgcagttt cagggaactg gcggagctgg gctcgagtcg cataatggtc cccttgccga
     5041 aggtggggtg gtggacgcgc atgccgacgc gatactcgag gacctcctgg gattcatcct
     5101 cataacgcgg catcggatcc gcctcccggc gcgggaagga agagcgctgc cggcggatcg
     5161 gggcggcgcg gtaagagctt tcgacctgta caagggcagg ttcgacctcg cggaggaaac
     5221 gcgaggcgat gctggctgag gtttcgccga acttgcgccg gttccgggcc caggagagat
     5281 agagtttaac cttggcgcgg gtggcgccta catagaaaag gcgcctttcc tcctcgagag
     5341 caggggggtc ggccagcgag cggctgatgg gaaagagccc ctcttcgagt ccagtgataa
     5401 agacgaccgg aaattccaga cccttggcgc tgtgcagggt catgagggtc acggcgttgg
     5461 cgcggtcgtc ccagctgtcg aggtcgctgg tgagggcgac attttcgaga aagccgtcga
     5521 gggtcgcctc gccgcctgcc gagctggtga attcggcgat ggcgagcaag agttcgcgga
     5581 cattctcggc gcggctgagg gattcgatgg tcccctcctg cttgaaatcg cggaggatgc
     5641 cgacctcatt gaccagggag gcggccagtt cagcgggaga gatctcctcg cgcagggcgg
     5701 tgtatttgtt caacagtccg tggaaagcgg ctacctgctc ggcggtgcgg gcggcgatgc
     5761 cgggaatctg ctggacctgg ccggtagcgg caaatatgga gatttttttc tcctgggcgt
     5821 aggcctcgat gcggccgagg gtggtttcgc cgatgccgcg tgcgggataa ttgataatgc
     5881 ggcgcaggct gatggtatcg gcggggttgc tgagcaaacg caggtaggcg atcacatcct
     5941 tgacctcctt gcgttcgtag aatttgacac cgccgacgat gatgtagggg atgttctcgc
     6001 ggcgcagggc ctcctcgaga acgcgcgact gggcgttggt gcggtaaagg acggcgatat
     6061 cctgaaaatc catatggttg gctcgcattt ccagggcgag tttgttgacc acggtgcgcg
     6121 cctcctcgag atcgtcctcc acttcgagca gggtgatgag atcgccctcg ggcttttcgg
     6181 tccagagctt tttctcgtgg cgctggcggt tgttgcgcac cactgagtgg gccacggcga
     6241 ggatgttcct ggtggagcgg tagttttgtt cgaggcggaa tttggcgcag tcgggatagt
     6301 cgttttcaaa atcgaggatg ttgcggatct ccgcaccccg ccagcgatag atggattggt
     6361 catcgtcgcc gaccacgcag atattgcggt ggcgggcggc gagcatcttg agagccacat
     6421 actgggcgtg gttggtatcc tgatactcgt cgacgaggat ataacggaag cgttcctgat
     6481 agtactcgag gacgagggga tagagctgga agagctcaat gggtttgatg agcaggtcat
     6541 cgaaatccat ggcattgttt tcgcgcagac gggtctggta ggtggagtag atagtcgcaa
     6601 tgatctggtc ttgccgggtt gcggccatcc gggcgaactg ttccggcagg atgaaggcgt
     6661 tcttggcgga gctgatcttg taagagatca tcttgggcgg gaaatcgttg acgggaatct
     6721 tgagatcgtt cagcaccgtc ttgatgaggc tgtgttgatc gtcatcatca tagatagcaa
     6781 aattggaggc gtaattgata cgttctgctt cgcggcgcag caggcgtgcg aagagggagt
     6841 gaaaggtgcc gatccacatg ccggagagca tctccgggac cagcttgcgc acgcgttctc
     6901 gcatctcccc cgccgccttg ttggtgaagg tcatggcgag gatttcgcgc ggctcggcca
     6961 gtccgttggc gagcagccag gcaatgcggt aggtgaggac gcgggtcttg ccgctgccgg
     7021 cgccggcgag aatgagcacc ggtccatcgg ttttggccac cgcctggctc tgggcgtcgt
     7081 tgagatcggc cgtcagccac tccggcgacg tccctttttt ttcactggct ctggtcacgc
     7141 tacttcctgt tcgcttgcga aaggcgtttt ctctcccggt cgaggacgct tttggccttg
     7201 agatggccgc gaaaattggc tgaaaaggta tgtccgcccc gggtatcggc gacaaaaaag
     7261 agataatcgg tcgcctccgg atggagggcg gccaggatgg cctcacggcc ggggttgccg
     7321 atggggcccg gcggcagacc gcggtagaga taggtattgt aaggcgaatc gatctccaga
     7381 tcgcggtaga ggaggcggcg cggtccgttg ggcagaagaa actggatggt cgggtccgcc
     7441 tgcaggggga tgccgagccg gagacggttg tgataaaccg aagagatggt gggcatttca
     7501 cggctcagtt tggcctcggc ctggataatt gaggcgagag tgacgatctc accggtggtg
     7561 aagcccattt cctgagagcg ctgatagagg gtatcccaga ccatcttttt gaactgctgc
     7621 accagcgggg tgatgacctg ggcctccgtc gagccatagg ggaagaaata ggtttcggga
     7681 tagagatagc cctccagatc ggcggtattt atcttgaagc gggcgatgaa actggaatcg
     7741 cgcgtcagct gaacgaaccg gaccgaatcc atcgccattt cacggcggaa gatggcggcg
     7801 atacggcgga cggtgagtcc ctccgggatg gtgactttgg tcaggatctg cggcccctcg
     7861 gtcagggcct ggatggcgcg cttgttggag agcggatagg gaatgcgaaa gtggccggcg
     7921 cgcagggctc cccccttgtt gagcagcttg agggcgagga ggaagcggct ggaactgccg
     7981 acaatcccgg cccgttccat catcacggcg atggaatctg gagaggtgcc gaatttgatg
     8041 cggatgtccg ctgcttgtcc ttgcgggggc tgccccagcg gccaatagag gatggagacc
     8101 agagggatag ccgcagcgag gagcaacagg accagcaggg cgacggccgc ccggcgaagc
     8161 gaaaagtgga atactttatt cgtcatactc tcccgtatct gaaggccggc ctgtgcagta
     8221 caaaccggcc catcgcatca aagtcaaaga atcaggaata aatcatagag ggcgtgactc
     8281 cagcccgcaa tgccgaggcc gcgaaaatgg tagatcagac agaaaagcag cccggccaga
     8341 aagcgaaaaa cggccgtata ccaggcaaag gcctcgtggc ccatataatg ggctgcggaa
     8401 aaaacggccg aggagagagc ggcgcagacc agatagatga tcgtcagggg gaggcgggtc
     8461 agatgggtca ggaccagggc catgccccca tagagaaata acctgaagac gatctcctcg
     8521 tagaccccgg cgccgagggc cagcatgagc gaggcgtgga atgaacgctc ctcccccatg
     8581 ctgagcatga tataatcggt cgcggtggtc aggaccaggg cc
//
LOCUS       DAOPWU010000130         8601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8208_length_8601_cov_5.360286, whole genome shotgun sequence.
ACCESSION   DAOPWU010000130 DAOPWU010000000
VERSION     DAOPWU010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8601
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8208_length_8601_cov_5.360286"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2355
                     /locus_tag="PKM23_08075"
     CDS             <1..2355
                     /locus_tag="PKM23_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNY91471.1"
                     /translation="NLDRYYYAPFHLVRSSDGTIVFSGSISRHRHKTAADFSSTTFSN
                     KNFTRADVWQCDFSSFNTPGEYRLVVEKMGCSYAFEIGGDVYRQAYHLTSRALFTQRQ
                     GVNKEIEPGLIYPRDHRTEDGIVMNYYPELKTEGNFDPNQGKGSVTGVWGWYHDAGDW
                     DTYPDHYVVPMTLLALYDLKPENFGDGDVGGKYQVDAGEAWIDEGRNGLPDLLDEAQW
                     LIKYYKRTREALIAQGYSTGGIPGYAGVDAGAESGTASWQDKRVMAMKGADSVLMAYR
                     YAACAAYLCTGLDKFAGGVHPESPAWIGEAKTAYDWAHAQNQDTNGDINRARMLAAAA
                     LYRTTGLTSYQEDFRSCKTRDDGWKSYLWYMINPWHHAALVFGRIPDGHPGLDRALRH
                     QCRDDLIAMADKETVQTTNDRGFRYGASRSIMHMLGSLSTPKIFLAAAAYEFSGEKRF
                     LDACYTTCDYCLGGNQLDLVRISGLGENPERQPFHCDSWYLTDYNSMVYDNPILPGYV
                     IYDIHYDGDWMSGENWTWIGDEDYSRSTAFPAIAAFPDAEARFPNRWSIAGSEFTIQQ
                     TQCQAIFAYGYLCGPNSGSFTPNQRPEVALNLEANSTVRLDSTLMLTVKASADVRRVE
                     YYYDYHFIGESSDREQNFAFAWKMADYQILRGRRLITAKAFDDLGMESRPTEAGQRTI
                     VCDQPSAVKKAEAGPLRFELKNCYPNPVNPATRIEFTLPAAGRTRLWVINLLGERVLT
                     LLDSPLAAGEHAVELDAASLPTGIYYYLLRQEASQQVKKMAVLR"
     gene            2727..4049
                     /locus_tag="PKM23_08080"
     CDS             2727..4049
                     /locus_tag="PKM23_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNY91472.1"
                     /translation="MITKCGRAMALLVCLLLTGTLLNAQVVDPEEVVKRQATDRTNSG
                     IERGVDEGFNQIEKGLGKLLGKKKQKAGEQNEEEAEAEASAETESGAEEESRRPPSSV
                     TPARQKLESASRYDFVPGDQVLFFEDFSQDGIGDFPALWTSNGSGEVKTVNIASGKWF
                     HMNGEDAVYCFAKKIEFPDNFIIEFDIIPDGEYTDGITLTLYAENPEEPQELNSDLYP
                     GTAGLHITIKKEGWETKGYASVDDAWLEGQAVRNPVLIEQVNHVIIWVQKRRVRIYHQ
                     GAKTLDVPTNIHAGVKFDRLRFSGWGAYSYPMISNFQITTASPDTRSKLLTEGKLVSY
                     GISFDVNKAAVKPESYGTLKSIADVLQENPGMNLSIIGHTDSDGDDTANLDLSKRRAE
                     SVKNELIKTFGIEAARLETGGAGESQPLAANDTPANKAKNRRVEFIKK"
     gene            4120..4836
                     /locus_tag="PKM23_08085"
     CDS             4120..4836
                     /locus_tag="PKM23_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91473.1"
                     /translation="MQEKSRKLLGLAVFAAAMGFLEAIVVVYLRALYYPEGFYFPLAA
                     MPGGIYLTEVLREICTLVMLAAIGWLGSRTPFTRFTCFLYLFGVWDLFYYLALKLLLD
                     WPASLLTWDVLFLIPITWIGPVIAPVLCALAMIVLHLAAERFLARGERSVFNRREWLL
                     LLAGALFVYVSFTIDYTLLIFRHDLTGGLTKVLRDENMVRTITQFIPSRFAWEIYIPG
                     LLLILAALLSFIRTERDGAA"
     gene            5007..6638
                     /gene="hcp"
                     /locus_tag="PKM23_08090"
                     /gene_synonym="priS"
     CDS             5007..6638
                     /gene="hcp"
                     /locus_tag="PKM23_08090"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008798258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNY91474.1"
                     /translation="MFCNQCQETVKNTGCTINGVCGKKEATANLQDVLIYACQGLALA
                     AIEARKQGIDTSVESRHITNSLFMTITNANFDDQAIIQAIRDSLKYRDALKNKVTLSV
                     RHDALVWHGDTEAEFHAKAADAGILAYDTDDNVRALKQYLLFGLKGMAAYTEHAFNLG
                     YEEQEIFTFMQEALAMITRPASLESALEWLMRTGEHGVKAMALLDKANTSTYGHPEIS
                     RVSIGAGKNPGILVSGHDLRDLEQLLIQSEGKGVDIYTHSEMLPSHAYPRLKKYKHLA
                     GNYGNAWHRQLDEFETFNGPILFTTNCLVPPRKTTTYNERVFTTGAAGMPGWKQIDKR
                     LPNGQKDFSAVIELARTCPPPTEIESGEITIGFAHNQVLALADKILAAVNSGAIKRLV
                     VMSGCDARQKSREYYTEFARQLPKDTVILTSGCAKYRYNKLNLGEINGIPRVLDAGQC
                     NDSYSWALVALKLKEILKLEDINQLPVVFNIAWYEQKAIIVHLALLALGIRNTHIGPT
                     LPAFLTPDLLRIVQEKFGVQTITTVEEDMKIFGLN"
     gene            6650..6829
                     /locus_tag="PKM23_08095"
     CDS             6650..6829
                     /locus_tag="PKM23_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNY91475.1"
                     /translation="MNDDEMICFCNEVTRGEIVKAIREKGLKSVEEVGEATLAGTTCG
                     GCQEEIQAILDELNR"
     gene            complement(6884..7951)
                     /locus_tag="PKM23_08100"
     CDS             complement(6884..7951)
                     /locus_tag="PKM23_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91476.1"
                     /translation="MPGLRGLLHRLLAIRFPLFCALFLAGLIPAAASPRPPAGGLSAD
                     SRSIKTLIELINRSEHDSVLALSRDLIGEDPASPLGYFMAADAYQTMMRDFRIKAWQT
                     EFDSLIAIASVKATTLLEREPTVENHFIAGVVQGYYCLALFGSGSYLRAIKTAENSIS
                     LLRKARQLDPGFADPLFGIAVYEYNKSKLLFGLLGGEEKAAVAKLRKVEKEGRYLSTN
                     ASYSLQATYLENGQYDSALVINDHLYKHYAASPSCLYHRALLLEKTGRLEEALQIWEQ
                     LIATLQRRQPVSNGFLAESHYHLAWLNRQLKRDENARKLLIQSARFAARRRADEELEG
                     SYIKFKEIKGKINSALAEWQQ"
     gene            8268..>8601
                     /locus_tag="PKM23_08105"
     CDS             8268..>8601
                     /locus_tag="PKM23_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91477.1"
                     /translation="MMHKSAAGSCLCAAVSHRGHPGLILPWLAVLALLGSTLAVAFAG
                     STGWVGTWSTAPQLVETNNNPPAPGLTSNTLRQILRVSIGGDTLRMRFSNEFSSAPVT
                     LQRVLIAAS"
BASE COUNT         1783 a         2631 c         2527 g         1660 t
ORIGIN      
        1 aatctcgatc gctattatta cgcccccttt cacctggtgc ggagctcgga tggtacgatt
       61 gttttttccg gctcgatcag ccggcaccgg cataaaacgg ccgccgattt ttccagcact
      121 accttcagca acaagaattt cacccgcgcc gacgtctggc agtgcgattt ctccagtttt
      181 aacacccccg gcgagtaccg cctcgtcgtc gaaaaaatgg gctgttctta cgccttcgag
      241 atcggcggcg atgtctaccg ccaggcctat cacctcacct cgcgggcgct cttcacccag
      301 cggcaggggg tcaacaagga gatcgagccg gggctgatct atccccgcga ccaccgcacc
      361 gaggacggga tcgtgatgaa ctattatccc gagctgaaga ccgagggcaa ctttgacccc
      421 aaccagggca agggcagcgt caccggggtc tggggctggt accatgacgc cggcgactgg
      481 gacacctatc ccgaccacta tgtggtcccg atgaccctgc tggcgcttta cgacctcaag
      541 ccggagaatt tcggcgacgg cgatgtcggc ggtaaatacc aggtcgacgc cggcgaggcc
      601 tggatcgacg aggggcgaaa cggcctgccc gatctgctcg acgaagcgca gtggctgatc
      661 aaatactaca aacggacgcg cgaggccctg atcgcccagg gctactccac cggggggatt
      721 cccggctatg ccggggtcga cgccggcgcc gaaagcggca ccgcctcctg gcaggacaag
      781 cgtgttatgg ccatgaaggg ggccgattcg gtgctgatgg cctaccgcta tgccgcctgt
      841 gccgcttatc tctgcaccgg tctggacaag ttcgccgggg gcgtgcatcc ggagagcccg
      901 gcctggatcg gcgaggccaa aaccgcctac gactgggccc atgcccaaaa tcaggacacc
      961 aacggcgaca tcaaccgcgc gcgcatgctc gccgccgccg cgctctaccg cactaccggc
     1021 ctcacctcct atcaggagga tttccgcagc tgcaagacga gagacgatgg ctggaaatca
     1081 tacctctggt acatgatcaa tccctggcac catgctgcgc ttgttttcgg ccgcatcccg
     1141 gatggccatc ccggactcga ccgggcgctg cgacatcagt gccgggacga cctcatcgcc
     1201 atggccgaca aggagacggt gcagaccacc aacgaccgcg gcttccgtta tggcgcctcg
     1261 cgcagcatca tgcacatgct cggctcactc agcaccccta aaatatttct cgcggccgcc
     1321 gcttatgaat tcagcggcga gaaaaggttc ctcgacgcct gctacaccac ctgcgactac
     1381 tgcctcggcg gcaaccagct cgatctggtc cggatcagcg gcctgggtga gaatcccgaa
     1441 cggcagccct tccactgcga ctcgtggtat ctgaccgact ataacagcat ggtctacgat
     1501 aacccgatcc tgccgggcta tgtgatctac gacatacact acgatggcga ctggatgtcg
     1561 ggggagaact ggacctggat cggggacgag gattactcgc gctccacagc tttcccggcg
     1621 attgccgcct tccccgacgc cgaagcgcgt tttcccaacc gctggagcat tgccggcagc
     1681 gagttcacca tccagcagac ccagtgccag gccatcttcg cctatggcta tctctgcggc
     1741 ccaaactccg ggagttttac tccaaaccag cggccggaag tggcattgaa tttggaggcg
     1801 aacagcaccg tccggctgga cagcacgctg atgctcacgg tcaaagcctc ggcggatgtg
     1861 cgccgggtcg agtattacta cgactatcat tttatcggcg agagcagcga ccgggagcaa
     1921 aactttgcct tcgcctggaa aatggccgat tatcaaatcc tgcgcggccg gcgcctgatc
     1981 accgccaaag cctttgacga cctcgggatg gaatccaggc cgacggaggc ggggcagcgc
     2041 acgatcgttt gcgaccagcc ctctgcggtc aaaaaggcgg aagccgggcc cctgcgcttc
     2101 gaacttaaaa actgttaccc caatccggtc aatccggcca cgcgtatcga gttcacactg
     2161 cccgcagcgg gaaggacccg tctgtgggtt atcaatctgc tgggggagag ggtcctgacc
     2221 ctgctcgaca gcccccttgc cgccggcgag cacgcggttg agctggacgc agccagcctg
     2281 cccaccggta tctattatta tctcctgcgc caggaggcct cccagcaggt taaaaaaatg
     2341 gctgtactca ggtaggatca ggccggcgct gctctcccgc aagggatcag ccgtgacccc
     2401 gaagctcagc ctttgcggaa tagtacttga cttccagcgc cggattggct aatttagtac
     2461 ggaattggat gtggggggag gttcaggcca gccggcctga aaagaggagt gcgatttgaa
     2521 cagtggtctc cggtctccct cggcagacat tgccctggtc tcctgcagcg tccgcagtac
     2581 agatttcccc ggtagtaaaa atcctgttgg tcagcatttc ccccgttccg ccggctcttc
     2641 cccgcgctca gggcatggga ccctctgcgc ctcccgtcgc atccccttcg tgaaccgggc
     2701 ttgatcatca gaagaaaggg tacgctatga taacgaaatg cggccgcgcc atggccctcc
     2761 tggtctgcct gctcctcacc ggcaccctcc tcaacgctca ggtggtcgat cctgaagagg
     2821 tggtgaagcg ccaggccaca gacaggacca acagcggcat cgagcgggga gtggacgagg
     2881 gttttaacca gatcgaaaag ggcctcggca agctgctggg caagaagaag caaaaggccg
     2941 gcgagcagaa cgaagaagag gctgaggcag aggcgtccgc agagacggaa tcaggcgctg
     3001 aagaagagag ccggcgcccg ccatcatcag tcacaccggc ccgtcagaag ctggagagcg
     3061 ccagccggta cgattttgtt cccggcgacc aggtgctctt tttcgaggat ttctcgcagg
     3121 atggaatcgg ggattttcct gccctctgga cctccaacgg cagcggtgaa gtcaagaccg
     3181 tcaacatcgc ctccggtaaa tggttccata tgaacggtga ggacgccgtt tactgctttg
     3241 cgaaaaagat cgagtttccg gataacttta tcatcgaatt cgacatcatc cccgatgggg
     3301 agtataccga tggcatcacc ctgaccctct atgccgagaa tccagaggag ccgcaagagc
     3361 tgaacagtga cctctatccc ggcactgccg gcctgcacat caccatcaaa aaggagggct
     3421 gggagaccaa aggctacgcc agcgtggatg acgcctggct ggaggggcag gcggtcagga
     3481 atccggtcct gatcgagcag gtcaaccatg tcatcatctg ggtccagaag agaagggtac
     3541 ggatctacca ccagggtgcc aagaccctcg acgtcccgac caatattcat gcgggagtga
     3601 aattcgaccg gctgcgcttc tcgggatggg gcgcctacag ttatccgatg atctccaatt
     3661 tccagatcac caccgcttcg cccgacaccc gcagcaagct cctcaccgag ggcaagctgg
     3721 tcagctatgg aatctccttc gacgtcaaca aggctgcggt caaaccggaa tcctacggca
     3781 ccctgaagag catcgccgat gtgctccagg agaatccggg catgaatctc agcatcatcg
     3841 gacatacgga cagtgacggc gatgacaccg ccaatcttga cctctcgaag cggagggcgg
     3901 aatcagtcaa gaacgagctg atcaagacct tcgggatcga ggctgctcgc ctggagaccg
     3961 ggggcgccgg ggagagccag ccgcttgccg ccaacgacac tcccgccaac aaggcgaaga
     4021 atcgcagggt ggaattcatc aaaaagtaac cggatggcgc tccccgcgac ggggagcgcc
     4081 acccctatct atcacccgtc ggccgggtga aggattcgca tgcaagaaaa atcccgcaaa
     4141 ctgctcgggc tggcggtttt cgccgccgcc atgggttttc tcgaagccat cgtcgtggtc
     4201 tacctccgcg ccctctacta cccggagggc ttttattttc cgctggcggc catgcccggg
     4261 gggatctacc tcaccgaggt gctgcgtgaa atctgcaccc tggtgatgct cgcggccatc
     4321 ggctggctgg ggagccgcac ccccttcacc cgcttcacct gctttctcta cctcttcggc
     4381 gtctgggacc tcttttacta cctcgccctc aagctgctgc tcgattggcc cgcctccctg
     4441 ttgacctggg acgttctttt tctcatcccc atcacctgga tcgggcccgt gattgcgccg
     4501 gtgctctgcg ccctggccat gatcgtcctc catctggcag ctgagcgctt cctggcgagg
     4561 ggagagagga gtgttttcaa cagacgggaa tggctgctcc tcctggcagg tgctctcttt
     4621 gtctatgtca gctttaccat cgattatacc ctgctgatct tccggcacga cctcaccggg
     4681 gggctgacaa aagtactccg ggacgaaaat atggtccgca ccatcacaca gttcatcccc
     4741 tcccgcttcg cctgggagat ttatatcccg ggcctgctgc tcatcctcgc cgccctgctc
     4801 tcattcatcc gcacggagag ggatggagcc gcctgaatcc ctgtatcagc tgtggtgctc
     4861 aaactgtgct gctgcgttgc cccggatgga aggggtgcgc cctccggttg gggagcaaaa
     4921 cttcttgatt atcagagcag tattttctaa attaattact tgtatttgca tgttaatcaa
     4981 catggattca tggaggtgat atgagcatgt tttgcaacca gtgtcaggag accgtcaaga
     5041 atacaggctg caccatcaat ggtgtgtgcg ggaagaagga ggccactgcc aatctgcagg
     5101 atgttctgat ctacgcctgc caggggctcg ctcttgccgc gatcgaggcc cgcaaacagg
     5161 gtatcgatac cagtgttgag agcaggcaca tcaccaacag cctctttatg accatcacca
     5221 acgccaattt tgatgaccag gccatcatcc aggccattcg cgacagcctg aagtatcgcg
     5281 atgccctgaa gaacaaggtg accctgagcg tccgccacga tgccctcgtc tggcatggcg
     5341 acaccgaggc cgaattccac gccaaagccg ccgatgccgg catcctggcc tatgacacgg
     5401 atgacaatgt ccgcgccctc aagcagtacc tcctcttcgg cctgaagggc atggccgcct
     5461 ataccgagca cgcctttaat ctgggctacg aggagcagga gattttcact ttcatgcagg
     5521 aggccctggc catgatcacc cggccggcca gcctggagag cgcgctggaa tggctgatgc
     5581 gtacgggcga acacggcgtc aaggcgatgg ccctgctgga caaggccaac acctccacct
     5641 acggccatcc cgaaatttcc cgggtctcca tcggcgccgg caagaatccc ggcatcctgg
     5701 tcagcggaca cgacctcagg gatctggagc agctgctcat ccagtcggaa ggcaagggcg
     5761 tggacatcta tacccactcc gagatgctgc cctcccatgc ctatccccgg ctgaaaaaat
     5821 acaaacacct cgccggcaac tatggcaatg cctggcatcg ccagctggat gaattcgaaa
     5881 ccttcaacgg tccgattctc ttcaccacca actgcctggt tcctccccgc aagaccacga
     5941 cctacaacga acgcgtcttc accaccggtg cagccggcat gccgggctgg aaacagatcg
     6001 acaagcgctt gccgaacggg cagaaggatt tttctgctgt catcgagttg gcgcggacat
     6061 gcccgccccc gaccgagatc gaaagcgggg agatcaccat cggtttcgcc cataatcagg
     6121 tgctggccct cgctgacaag atccttgcgg cggtcaattc gggggcgatc aagcggctgg
     6181 tggtcatgtc cggctgcgat gcccggcaga agagccgcga atactatacc gagttcgccc
     6241 gacagctgcc gaaggatacc gtgattctca cctccggctg cgccaagtac cgctacaaca
     6301 agctcaatct cggcgagatc aacggcatcc cgagggtgct ggacgccggc cagtgcaacg
     6361 actcctattc ctgggctttg gtggcgctca agctcaagga gatcctcaag ctggaggaca
     6421 tcaaccagct gccggtcgtt ttcaatatcg cctggtatga gcagaaagcg atcatcgtcc
     6481 atctcgccct gctcgctctc ggcatcagga atacccacat cggaccgacc ctgccggctt
     6541 ttctgacgcc cgatcttctc cgcatcgtcc aggagaaatt tggcgttcag accatcacca
     6601 cagtggaaga ggacatgaag atctttgggc taaactgagg ataaccggta tgaatgatga
     6661 tgaaatgatc tgcttctgca acgaggtcac ccggggggag atcgtcaagg cgatcaggga
     6721 aaaggggctg aagagcgtcg aggaggtcgg cgaagcgacc ctggcgggca cgacgtgcgg
     6781 gggctgtcag gaggagatcc aggccatcct tgacgaactg aaccgttaac tctgcatcag
     6841 gcgccggggc ctcgtgatcc ctcgtgacaa ggctccggcg gtttcactgc tgccattccg
     6901 ccagcgcaga attgatcttg cccttgatct ccttgaactt gatataagag ccctccaact
     6961 cctcatcggc gcggcgccgg gcggcgaagc gggccgactg gatgagcagc ttgcgggcgt
     7021 tctcatcccg cttgagctgg cggtttagcc aggccagatg atagtggctc tctgcgagaa
     7081 agccgttgct gaccggctgg cgacgctgca gcgtcgcgat cagctgctcc cagatctgca
     7141 gagcttcctc gagacggccg gttttttcaa gcaggagggc gcggtggtag agacaggagg
     7201 ggctggcagc atagtgttta tagagatgat cattgatgac caaagcgctg tcgtattggc
     7261 cgttctcgag ataggtcgcc tgcaaagaat agctggcatt ggttgacaga tagcgcccct
     7321 ccttttccac cttcctcagt ttggcgaccg ccgctttctc ctctccaccc agcagtccga
     7381 acagcagctt gctcttgttg tactcataga cggcgatgcc gaagagggga tccgcaaagc
     7441 cgggatcgag ctggcgggcc ttgcgcagca gggagatgct gttttccgcg gtcttgatcg
     7501 cgcgcagata gctgcccgag ccaaagaggg cgaggcagta gtagccctgc acgacgcccg
     7561 cgatgaaatg gttttccacg gtcggttccc tctccagcag ggtggtggcc ttgacggagg
     7621 cgatggcgat caggctgtcg aattcggttt gccaggcctt gatgcggaaa tcgcgcatca
     7681 tggtctggta ggcatcggcg gccatgaagt agccgagcgg gctggcggga tcctctccga
     7741 tcagatccct ggatagggcg agcacgctgt catgctcact ccggttgatc agttcgatca
     7801 gggtttttat gcttcgggaa tctgcagaga ggccgcctgc gggcggccgg ggcgaggccg
     7861 cggccggaat cagtccggcg agaaaaagcg cgcagaaaag gggaaagcgg attgcaagga
     7921 ggcggtggag aagccccctc aggccaggca agttcttgct cgggtacatg gatgcacttt
     7981 ctgttcagga ccaggcgccg gtcaggtcgt gagggagaga gcgggagccg gttactcctt
     8041 tctcgggcgc gaatcgatgt ttaaagtaac cattcagcgg cgtaataaca aggagattgt
     8101 cggcggcagg agacacgggg cgcagataca gtcgggcggg ttttcccgga tgccggctgc
     8161 agccgaaagg cggctgcggc gaatagcctt gtttttgaac ggctgaatgg ctattttcag
     8221 gcgggggaaa ttcgactgtt tgcatgctga aaaagggaga gacgaaaatg atgcataaat
     8281 ccgcagcggg atcttgcctg tgcgctgctg tttcacaccg gggacatccg ggcctgatcc
     8341 tgccctggct ggcagtgctg gccctgctcg ggagcaccct ggccgtggcc ttcgccggat
     8401 cgaccggatg ggtcgggacc tggagcacgg ctccccagct ggtcgagacc aacaacaatc
     8461 cgcccgcccc cgggctgacc agcaacaccc tgcgccagat cctccgcgtc tcaatcggcg
     8521 gcgacaccct gcgcatgcgt ttcagcaatg agttcagctc cgccccggtg accctgcaaa
     8581 gggttctgat cgccgcctcg g
//
LOCUS       DAOPWU010000131         8589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8226_length_8589_cov_5.182915, whole genome shotgun sequence.
ACCESSION   DAOPWU010000131 DAOPWU010000000
VERSION     DAOPWU010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8226_length_8589_cov_5.182915"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PKM23_08110"
     CDS             <1..316
                     /locus_tag="PKM23_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91478.1"
                     /translation="LTADDLHQLLDGLVHQGLLEQEIVSPRNEFTFMTPLGGKGIEMS
                     RLNALNRVYRYRSRIDQEHMMRFLQAAHYYVSATSRPDSAALTSQIRGHIQKLLMTAP
                     QP"
     gene            complement(332..1312)
                     /locus_tag="PKM23_08115"
     CDS             complement(332..1312)
                     /locus_tag="PKM23_08115"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNY91479.1"
                     /translation="MKVLVTGSNGFIGSTLVERLLREGHSVRCLVRRSSNLTWLNDLP
                     VEYVFADLNDAGELGGMVADVERLYHLAGVTKARDEAGYLRGNYEATLQLLAACRDSG
                     RRDLRFIYVSSQAAAGPSAAGRALSEEDPPRPISLYGRAKLRAEEAVQEYAREGFATI
                     VRPPSVYGARDRDIYVYFKSVARGLLLLLGDGRMKISLIHVDDLVEGILLAGESERAR
                     GRIYFLSSDEASDWRAIGSIIASAMQKKPLVVHVPVGLLKPVAWMSQLGSKITGVPAL
                     LNPDKVTKMLQPNWICSNARARAELGFRPRISLEEGMTATAAWYKEMGWL"
     gene            1652..2845
                     /locus_tag="PKM23_08120"
     CDS             1652..2845
                     /locus_tag="PKM23_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNY91480.1"
                     /translation="MADLFDKCKSATVSARAREAMEQGWYPYFKAIDSGADTEVMIKG
                     HRLIMIGSNNYLGLTQDPRVKAASMAAIDRFGSGCTGSRFLNGTLALHEELEARLADF
                     MKKEAVLIFSTGFTTNQGTIASLVGRKDLVVGDAENHASIVDGTRLAFGQAYKYRHND
                     MADLDRVLTNNKKNHGALVVSDGVFSMGGDIVDLPNMVKVAKKHGARIMIDDAHSLGV
                     LGAHGRGTAEHFGLEEEVDLIMGTFSKSFASIGGFIGGPEEVVHYIKHTARALIFQAS
                     PPPAAVAAVIAALDILISEPERRERLWKNVHKMKQGYQQLGFNTGVSQTPVIPLLIGD
                     DEKTFNFWKALYEAGIFSNPVISPAVPPGHSLIRTSYMATHTDEELDKVLEICGKVGK
                     KLGII"
     gene            complement(2951..3286)
                     /locus_tag="PKM23_08125"
     CDS             complement(2951..3286)
                     /locus_tag="PKM23_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91481.1"
                     /translation="MIKKDLELLVSLQDIDLMIEEVGDVEKLGFSVTGYTELKKAREE
                     LSASISKPLLFRYERLRGKLKRAIVPVKNDICLGCFLRQPTSIGVKGREDEQIFTCEN
                     CGRMLYWLD"
     gene            complement(3290..3751)
                     /locus_tag="PKM23_08130"
     CDS             complement(3290..3751)
                     /locus_tag="PKM23_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNY91482.1"
                     /translation="MHRSELAKYFKDNLFVASLQAAKKEVAIAELLDTFVREKYIRNR
                     DIVLEMLHQRETLGSTGIGKGVAIPHGRTTAAADVLIAFGKSDQGIDFDAIDGKPVHL
                     FFMVIAPPNEEGNVYLPILGSLVTILNEKANRDKLMKVETFQDFIAIITGE"
     gene            complement(3826..4164)
                     /locus_tag="PKM23_08135"
     CDS             complement(3826..4164)
                     /locus_tag="PKM23_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91483.1"
                     /translation="MGRGKAQTQQPAKSALNMSQEETLLALETLAKTLAVEVRYEKGD
                     FNGGLCRIDDRPILLVQKTDPLHRKIHVLARELGAMNLDHIFVMPALRALIEEEAASV
                     QPQAEAAPGK"
     gene            complement(4116..5195)
                     /locus_tag="PKM23_08140"
     CDS             complement(4116..5195)
                     /locus_tag="PKM23_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNY91484.1"
                     /translation="MENTYALIMAGGVGARFWPKSRKSLPKQYLTLVGKETLIQAVSR
                     RLQVIIPVDRIYVVSTKNQIPLIHAQMPWLPPSQLVIEPFGKNTAPCIGLAALHLIRH
                     DPEAVMMVLPSDHLITDTEKFAQILREAVDLVKGNPEALATIGIEPTYPATGYGYIQR
                     GAALDLPSGKAFRVRAFAEKPTHEVAEQFFSTGEFLWNSGIFIWRAATILHLIEDLMP
                     DLAAGLREIDQAIGTPKEAEITERVYKQIHSDSIDYGIMEHASNVMVLEGNFGWSDVG
                     SWQEVYKISPHDENGNILVNDPIVKECRNCYIEAGGRVVAVIGAEDLIVVDTPDALLI
                     CKQDQSQEVKWVVEKLKHSSQQKVL"
     gene            complement(5206..5529)
                     /gene="trxA"
                     /locus_tag="PKM23_08145"
     CDS             complement(5206..5529)
                     /gene="trxA"
                     /locus_tag="PKM23_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNY91485.1"
                     /translation="MGNAIEVTDATFAKEVLESSVPVLVDFWAVWCGPCKMIAPVVEE
                     VAAAKAGKMKAGKIDVDKNHQVATRYGIRSIPTLLLFKNGQVVEQIVGVVSKAALLSK
                     LEPHL"
     gene            complement(5625..6563)
                     /locus_tag="PKM23_08150"
     CDS             complement(5625..6563)
                     /locus_tag="PKM23_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY91486.1"
                     /translation="MTATILRLMRVEDLPGVNALLIRAFSTARRDEGFSCPALALCRM
                     EFLRFYQQDTPGSCWVAEAEGRLCGVVFGHGWGRAGWIGPLAVSPECQKRGLGRALLE
                     RCTDELRGLGCRLVGLETDPASLNNLAFYSVLGCTPGPLLVDLVHEIEPGWVVETEPL
                     LISYGQEPAPFESHLPAFLEANGVEADYLHLARLLQQHRFGESWLEMEGDQVRLFAAL
                     QIIPVSVQERAGVGRIMAVVGPGGTGADRLEAFIHSAAARSGCSHVVVRLSTWHMELL
                     AALLRRGWRLINSHLRFYITGGEKRGNGGMHLNKWD"
     gene            6745..8457
                     /locus_tag="PKM23_08155"
     CDS             6745..8457
                     /locus_tag="PKM23_08155"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNY91487.1"
                     /translation="MLTYEKRTDRFSGEEYIEVPFQGELLTEHPIYNKGTAFPEDERQ
                     SLGLCGLLPEAVSSLALQKQKTYESFCAKPDELEKYIFMLSLLDRNETLCYSLILDHL
                     EEMLPIVYTPTVGKACQQFSHLFRRRRGLYITANNIHRVDDIFRNAPFSNISLIVVTD
                     GERILGLGDLGSNGMGIPIGKISLYVAAAGLHPAYCLPIQIDVGTNNESLLADPLYIG
                     WRHNRLTGDAYDAIIEQFVNAVRRHFPHALLQWEDFGKGNAFRMLERYRERICSFNDD
                     IQGTGSVATAVLLSAMKIKKQKLGDQRFVMFGQGQAGLGIARQICTGLMMEGLSREEA
                     ANHIFGIDKDGLLLKGMPMSDEQQMFAKDPAFVANWHVADRSHITLLETIRNAKATVL
                     FGVTGQSGAFNEEVLKAMGANDPQAMIMPLSNPTVKAECTPEQAVAGAGPHCLIATGS
                     PFKPLNVNGAEKVISQCNNLYIFPGVGLGALICGTPKVTNEMFMAASQALSDLLSEEE
                     LKGGRMLPRIDKIRYVSAQVALAVAKEARRSGLGVRADDEKLLQMVMNAMWEPKYLPY
                     RLPE"
BASE COUNT         1825 a         2745 c         2329 g         1690 t
ORIGIN      
        1 gctcacagcg gacgatcttc atcagctcct cgacggcctg gtccatcagg gcctgctgga
       61 acaggagatc gtctcccccc gtaacgaatt caccttcatg accccgctgg gcggaaaggg
      121 gatcgagatg agccggctca atgccctcaa ccgcgtctac cgctatcgca gccgcatcga
      181 ccaggagcac atgatgcgct tccttcaggc cgcccattac tacgtcagcg ccacctcgcg
      241 cccggacagc gccgctctca ccagccagat ccgcggccat atccagaagc tgctgatgac
      301 cgctccccag ccttgaccgc ctccaccctt cctacagcca gcccatctct ttgtaccatg
      361 cagcggtcgc cgtcatcccc tcctcaaggc tgatgcgcgg cctgaagccc agttccgcac
      421 gggcgcgggc gttgctgcag atccagttcg gctgaagcat cttcgtcact ttgtcgggat
      481 tgagcagggc aggcaccccg gtgatcttgc tgcccaactg gctcatccag gcgaccggct
      541 tgagcagacc gaccggcaca tgcaccacca ggggcttttt ctgcatggcc gaggcgatga
      601 tcgagccgat ggcacgccaa tcgctggctt cgtcggagct gagaaaatag atccggcctc
      661 gcgcccgctc ggactcaccg gcgaggagta tcccctccac caggtcatca acatggatca
      721 gactgatctt catcctgccg tccccaagaa gaagcaaaag tccccgggcg acggacttga
      781 aatagacata gatatcgcgg tcgcgcgctc cgtagaccga gggcgggcgc acgatggtcg
      841 caaagccctc ccgggcatac tcctgaaccg cctcttcagc gcggagtttg gcccggccgt
      901 aaagggagat cggccgcggg ggatcctcct cgctcagggc ccggccggct gcactgggac
      961 cggcagccgc ctggctggag acataaatga agcgcagatc cctcctccct gaatccctgc
     1021 acgccgccag cagctgcagg gtcgcctcat agttgcctct gaggtagccg gcctcatcac
     1081 gtgccttggt gaccccggca agatgataga ggcgctccac atccgcaacc atgccgccca
     1141 gctcgccggc atcattcaga tcagcaaaga catattccac cggcagatcg ttcagccagg
     1201 tcagattgct gctccggcgc accaggcagc gcacactgtg gccctcacgg agcaggcgct
     1261 ccaccagggt gctgccgata aaaccattgc ttcctgtgac cagaaccttc atcgcttttt
     1321 ccgccctatt tatcaggcac aaattacatc attcgcgcgc caatttcaag taaaagcaga
     1381 ccacagctcc gtctcgagcg ggaaacggct ctccgagcca ttaatccgtt ccgcgcagca
     1441 ggacctcgta ttaaaaggag tttaaacttg actttgagcc gggcattggc tatattttaa
     1501 ccgaaacatc acccggtttg gcaaccgaac atcatttcta catgactgcg ggccatagcc
     1561 ccacctttcg atcattacca tcaccaggaa tatagttcaa gacccggcaa cgggcccagg
     1621 gcgtttcagt tttagtatgg aggcaccctc attggcagac ctgttcgata aatgcaaatc
     1681 cgccacggtt tcagcccgcg cccgcgaggc tatggaacag gggtggtatc cctatttcaa
     1741 ggcgattgat tccggcgccg ataccgaggt gatgatcaag ggccaccggc tgatcatgat
     1801 cggttccaat aactacctgg gactcaccca ggatccgcgc gtcaaagccg cctccatggc
     1861 cgccatcgat cgctttggat cgggctgcac cggttcgcgc ttcctcaacg gcaccctcgc
     1921 cctgcatgag gagctggagg cccgcctcgc cgatttcatg aagaaagagg cggtgctgat
     1981 tttctccacc ggcttcacca ccaaccaggg caccatcgca tccctggtcg ggcgcaagga
     2041 cctggtggtc ggcgacgccg aaaaccacgc cagcatcgtc gacggcaccc gcctcgcctt
     2101 cggccaggcc tacaagtacc gccacaacga catggccgat ctcgaccgtg tcctcaccaa
     2161 caacaaaaag aatcacggcg cgctggtcgt cagcgacggg gtcttcagca tgggcggcga
     2221 catcgtcgat ctgcccaaca tggtcaaggt ggccaaaaag cacggggcgc gcatcatgat
     2281 cgatgatgcc cactccctcg gggtgctggg cgcccacggc cgcggcaccg cggaacattt
     2341 cggcctcgag gaggaggtcg acttgatcat gggcaccttc agtaaatcct tcgcctcgat
     2401 cggcggcttt atcgggggac cggaggaggt cgttcactat atcaagcaca cagcccgcgc
     2461 cctgatcttc caggccagcc cgccgcccgc cgctgtcgcc gcggtgatcg ccgcgctgga
     2521 tatcctcatc agcgagcccg aacgccgcga acggctctgg aaaaatgttc acaaaatgaa
     2581 acaaggctac cagcagctcg gctttaatac cggcgtaagc cagaccccgg tcatcccact
     2641 cctcatcggc gacgacgaaa aaaccttcaa cttctggaaa gccctttatg aggcgggcat
     2701 attctccaat cccgtcatca gcccggcggt gccgcccggc cactccctca tccgcaccag
     2761 ctatatggcc acccataccg atgaagaact ggacaaggtg ctcgagatct gcggcaaggt
     2821 gggcaagaag ttggggatca tctaagcgca gcggcagcgt acaactgttg cagaaaaaaa
     2881 ggccactctg agagtggcct ttgcttttcc atccgcagac gctccggcag tgggagcgga
     2941 catacggcat tcaatccagc cagtagagca tgcgtccgca gttctcgcag gtgaaaatct
     3001 gctcatcctc gcgtcccttg accccaatgg aggtgggctg gcgcaggaaa cagccgaggc
     3061 aaatatcatt cttgaccggc acaatcgccc tcttgagctt gccgcgcagg cgttcataac
     3121 ggaagagcag gggtttgctg atgctggccg aaagttcctc ccgcgccttt ttaagctccg
     3181 tatacccggt gactgaaaag cccagctttt ccacatcgcc gacttcttca atcatcaggt
     3241 cgatatcctg gagggaaacc aggagttcca gatctttttt gatcatcgat cactctcctg
     3301 taataatggc gataaaatcc tgaaaggtct cgaccttcat cagcttgtcg cgattggcct
     3361 tttcgttgag gatggtgacc agggagccga gaatgggcag gtagacgttc ccctcctcat
     3421 tgggcggcgc gatcaccata aaaaagagat gtacgggctt gccgtcgatg gcatcaaagt
     3481 cgatgccctg atccgacttg ccaaaggcaa tgaggacatc cgccgcagcc gtggtcctgc
     3541 cgtggggtat ggctacgccc ttgccgatgc cggtactgcc aagggtttca cgctggtgga
     3601 gcatttccag gacaatgtcg cggttgcgga tgtatttttc ccggacaaag gtgtcgagca
     3661 gctcggcaat ggccacctct tttttcgccg cttgcaggct ggccacgaag aggttgtcct
     3721 tgaagtactt ggccaattcg gatctgtgca tatcatccct ccatggaggt tagggccctt
     3781 aaaaaccgac aggatgcata tctgaaccgg tggggtgcgc cccggtcatt tacccggggc
     3841 ggcctccgcc tgaggctgga cagaagcggc ctcttcttcg atgagggcac gcagcgccgg
     3901 catcacaaag atatgatcaa gattcattgc ccccagttca cgcgccagaa catggatctt
     3961 gcgatggagc ggatcagttt tctgcaccag taggatgggg cggtcgtcaa tccggcacaa
     4021 tccgccgttg aaatcgcctt tttcatagcg cacctcgaca gcgagggttt tcgccagcgt
     4081 ttccagggcg aggagggtct cctcctggct catgttcaaa gcactttttg ctggctgctg
     4141 tgtttgagct tttccacgac ccattttact tcctgcgatt ggtcctgctt gcagatcagc
     4201 agtgcatcgg gggtgtcaac cacaatgaga tcctcggcgc caatcacggc gaccacacgc
     4261 ccgcccgctt cgatgtaaca gttccggcac tccttgacaa tcgggtcatt gaccaggatg
     4321 ttgccgttct cgtcgtgagg ggagatcttg tagacctcct gccagctgcc gacgtcactc
     4381 cagccaaaat tgccttcgag caccatgacg ttgctggcgt gttccatgat gccataatcg
     4441 atcgagtcgc tgtggatctg tttgtagacc cgctcggtga tctccgcctc cttcggggtg
     4501 ccaatggcct gatcgatctc ccgcaggccg gccgccagat cgggcatcaa atcctcaatg
     4561 aggtggagaa tggtagccgc ccgccagata aagatgccgc tgttccagag gaactcaccg
     4621 gtcgaaaaaa actgctcggc cacctcatgg gtaggttttt cggcgaaagc ccgaacccgg
     4681 aaggccttgc cggagggcag gtcgagcgcg gcaccgcgct ggatatagcc ataccccgtc
     4741 gccggatagg tcggctcgat gccgatggtg gccagggcct caggattgcc cttgaccaga
     4801 tcgaccgcct cgcggaggat ctgggcaaac ttttccgtat ccgtgatgag atggtcggag
     4861 ggcagaacca tcatcacggc ctccggatcg tgccgtatca gatgaagggc cgccaacccg
     4921 atgcaagggg cggtgttctt gccgaagggt tcaatgacca gctgtgaggg ggggagccag
     4981 ggcatctgcg cgtggataag aggaatctga ttcttggtgg agacgacata aattctgtcg
     5041 accgggatga tcacctgcag ccggcggctg accgcctgaa tcagagtctc cttgcccacc
     5101 agggtgagat actgcttggg cagggacttg cgactctttg gccaaaagcg ggctcccaca
     5161 cctccggcca tgatgagtgc ataggtattt tccataacgc gcattttaga gatggggttc
     5221 gagtttgctc aaaagcgccg ctttcgaaac caccccgacg atctgctcca ccacctgccc
     5281 gttcttgaag agcagcaggg tgggaatgct gcgaatgcca tatctggtcg caacttgatg
     5341 atttttatcc acatcaatct tgccggcctt catttttccc gccttggccg cagccacctc
     5401 ctccaccacc ggtgcgatca ttttacaggg tccgcaccat accgcccaga aatcgaccag
     5461 cacaggcaca ctggattcca gtacctcttt tgcgaaggtc gcgtctgtca cctcaatggc
     5521 atttcccatc tcttctccct gtttggtaat aacctgtact catatcccgc caaattaaga
     5581 agaattgacg ttttatgcaa cagaaaactg tgctgaaaag ggggctaatc ccacttattg
     5641 agatgcattc ccccattccc gcgcttttcg ccgccggtga tgtaaaagcg cagatgggag
     5701 ttgatcaggc gccagccccg acgcagcagg gcggcgagga gttccatatg ccaggtggag
     5761 aggcggacca ccacatggga gcagcctgac ctggcggcgg ccgagtgtat aaatgcttcc
     5821 agcctgtcag ccccggtccc gccgggtccc accaccgcca tgatccgccc cacccctgcc
     5881 ctctcctgca ctgaaaccgg aattatctgc aacgcagcga acaggcgcac ttgatcccct
     5941 tccatctcca gccaggactc cccgaaacga tgctgctgca gcagccgggc gaggtgcaga
     6001 taatccgctt cgaccccatt ggcctccaga aaggccggca gatggctttc aaagggagca
     6061 ggttcctggc catagctgat gagaagcggc tcggtctcca ccacccagcc cggttcgatc
     6121 tcatgcacca gatcgaccag aaggggcccg ggggtgcagc cgagtacgct gtagaaggcg
     6181 agattgttga ggcttgcggg atccgtctcc agaccgacca gcctgcaacc cagcccgcgc
     6241 agctcatccg tgcagcgttc cagcaacgcc cggcccaggc cccttttctg acactccgga
     6301 gataccgcca gggggccgat ccagcctgcc cgcccccagc catgcccaaa gaccacaccg
     6361 cagagcctcc cctctgcctc tgccacccag cagctcccgg gggtatcctg ctgataaaag
     6421 cgtaaaaatt ccatgcggca aagcgccagc gccggacagc taaagccctc atcccgacgc
     6481 gcggtcgaaa aggcccggat cagcagggcg ttcacccccg gcagatcttc aacccgcatc
     6541 aggcgcagaa tggtcgcggt catcgctttc ttcctcacac aatgcaatcc ccattcagca
     6601 agttaaacaa tgatcatcaa accggcaagg agataattcc ctctctggtg caggttcagc
     6661 ttgacagata gaattggatt tgctatcttg tagagggtac accaaagccc agtatgaatt
     6721 ttatccatat cgaagggaga ggctatgctg acctatgaga agcgaaccga ccggttcagc
     6781 ggcgaagagt acatagaagt ccctttccaa ggagaactcc tcaccgaaca ccccatctat
     6841 aacaagggaa ccgcttttcc cgaggatgag cgccagagcc tgggcctctg cggcctgctg
     6901 cccgaggcgg tatcctcgct ggcgctgcag aaacaaaaga cctatgaaag cttttgcgcc
     6961 aagccggatg aactcgaaaa gtacatcttt atgctctccc tgctcgaccg gaatgagacc
     7021 ctctgctact ccctcattct cgatcatctc gaagaaatgc tgcctatagt gtacaccccg
     7081 actgttggca aagcctgcca gcagttcagc cacctcttcc gccgtcgccg cggcctctat
     7141 atcaccgcca acaacatcca ccgcgtcgat gacatcttcc gcaacgcccc cttctccaac
     7201 atctccctga tcgtggtcac cgatggcgag cgcattctcg gactgggcga cctcggcagc
     7261 aacggcatgg gcattcccat cggcaagatc agcctctacg tcgccgccgc cgggctccat
     7321 cccgcctact gcctgccgat ccagatcgac gtcggcacca ataacgaaag tctgctcgcc
     7381 gatcccctgt acatcggctg gcgtcataac cgcctcaccg gcgatgccta cgacgccatc
     7441 atcgagcagt tcgtcaacgc cgtccgccgc cattttccgc atgccctgct gcagtgggag
     7501 gatttcggca agggcaatgc cttccgcatg ctcgagcgct acagggaacg catctgctcc
     7561 ttcaatgatg acatccaggg taccggctcc gtggccaccg ccgtgctgct ctcggcgatg
     7621 aaaatcaaga agcaaaaact gggcgatcag cgctttgtca tgttcgggca gggacaggcg
     7681 ggcctcggca tcgcccgcca gatctgcacc ggcctgatga tggaggggct gagccgcgag
     7741 gaggcggcca accatatctt tggcatcgac aaggatggac tgctcctcaa gggcatgccc
     7801 atgagcgatg agcagcagat gttcgccaag gatcccgcct ttgtcgcgaa ctggcatgtg
     7861 gccgatcgca gccacatcac cctgctcgaa accatcagga acgccaaagc cacggtgctc
     7921 ttcggcgtca ccggccaaag cggcgccttt aacgaggagg ttctcaaggc catgggtgcc
     7981 aatgatcccc aggcgatgat catgcccctc tccaatccta ccgtcaaggc cgagtgcact
     8041 cccgaacagg cggtcgccgg cgccggtccc cactgcctca tcgccaccgg cagccccttc
     8101 aaacccctca acgtcaacgg cgccgagaag gtcatctccc agtgcaacaa cctctacatc
     8161 tttccgggcg tcggactcgg cgccctgatt tgcggcaccc ccaaggtgac caatgagatg
     8221 ttcatggccg catcccaggc cctgagcgat ctgctctcgg aggaggagct gaagggcggg
     8281 cgcatgctgc cgcggatcga caaaatccgc tacgtctccg cccaggtggc cctggcagtg
     8341 gcgaaggagg cgcgccgcag cggcctcggc gtccgtgccg acgatgaaaa actcctgcag
     8401 atggtgatga atgccatgtg ggaaccgaaa tatctgccct atcggctgcc cgaataggac
     8461 gagggcggct taataaaaag agcggcatcc ctgacgggat gccgctcttt ttaattgctg
     8521 cggaccagat tggctggaag cgggcctggc gtggtcatgg attaccctcc cgctcactcc
     8581 tttaccaac
//
LOCUS       DAOPWU010000132         8527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8295_length_8527_cov_5.096199, whole genome shotgun sequence.
ACCESSION   DAOPWU010000132 DAOPWU010000000
VERSION     DAOPWU010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8295_length_8527_cov_5.096199"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PKM23_08160"
     CDS             complement(<1..741)
                     /locus_tag="PKM23_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNY91488.1"
                     /translation="MNAKELKTQEYAEKLNEISDTMQSLIWKMISELRESEQRYRSIV
                     EFAADAIITMSKDTHVTSWNKGATAIFHYAEEEAIGRPIDSLIAKNGVSQEAARLSAQ
                     VLEGASLQSLEAMRYTREGEPRHVLISAAPITGEDGSVEEVSLIYKDITDLKQAHEQL
                     LRTEKQATLGIIAGSIGHELNNLVGGLLLHAQVLKEVDKEPEKVREIADLFCANLEKV
                     ALHGKNLLSLSRPTSPRFEPMDINAVINE"
     gene            complement(952..1929)
                     /locus_tag="PKM23_08165"
     CDS             complement(952..1929)
                     /locus_tag="PKM23_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNY91489.1"
                     /translation="MAKSKKEWVVGIDLGGTKIYAAVIDRKGTILGSERKKTKSELGF
                     PATVSRMADCARQAVVNAGVSWDGIRAVGVGSPGPLDMKKGMIIESPNLAWKEAPLKK
                     ELKRLLKKPVYVDNDCNVGILGEHAYGAAREARDVIGFFVGTGIGGGIIIGGKLLHGF
                     NENAGEIGHVILDPHGPRCGCGNNGCLEAFASRLSIEREIRAAIAAGTPSPLFEGVEN
                     PDAPIRSRRLLEAYLAGDPAVVPAINRSAEYLGYATAGMLNIFNPEVVVIGGGVVEAL
                     GALYVDRVREVAVANCFKISSRNVRIVEAALKDDSAVLGAAVLAWSHCR"
     gene            complement(1990..3165)
                     /locus_tag="PKM23_08170"
     CDS             complement(1990..3165)
                     /locus_tag="PKM23_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91490.1"
                     /translation="MMLGLLAAALIAAKGSAQTRPVVTDEKTRQELTQLLREICDWAI
                     AVELRTGEIKIAEKRRTSIFINSNLARVLLAGYQICGEERYREEALAWFDRLVELQQV
                     TLSAKGDTVGWWGDMAPDANIYLGDAGTSATGLAGAVRFASAPRRAAYLRALRRYANF
                     VRHGCAEDPQGKGRGGSPGWVIETGPNRGALGTGYYKGQLAVAPYTIATAVTGCGFFS
                     ALYGLTRAPEDIRTAEAAGKWLLLQRAPDGHIPYTIENRIHEEWPINTLSYVSDGLIG
                     LYRRTPSTAMQEEIAASINRSINWLITQQNNQGLWGKMRSEDQQRSQGAINLMVLYYS
                     SISPEKMVLESIRRNYSFFLKPGNPAKFGLFELSISTGFAGLSIAEVLEPGITYRID"
     gene            3539..3775
                     /locus_tag="PKM23_08175"
     CDS             3539..3775
                     /locus_tag="PKM23_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91491.1"
                     /translation="MSAEKKALYIIRENCGDVAQRIRACRSRQIALQLRQCLCRELEN
                     SCTSPEMKRLLQNHINELITDTFDENGMNRYLEA"
     gene            3775..4317
                     /locus_tag="PKM23_08180"
     CDS             3775..4317
                     /locus_tag="PKM23_08180"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNY91492.1"
                     /translation="MKRGMIFIATALILATGVQTGCTKQKEKANAAVPVKEAASSGLK
                     SAPAFELAGTDGKTIKLSDYKGKVVIVDFWDTWCPPCRMEIPHFIELQNQYKSKGFEM
                     IGVVLGREGKEKVLDFIKSNKINYVNAYATEAFLNAYGPIEGIPTTFVIDQQGRIYKS
                     YTGYRDKAVFENDIKALLKL"
     gene            4329..4535
                     /locus_tag="PKM23_08185"
     CDS             4329..4535
                     /locus_tag="PKM23_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNY91493.1"
                     /translation="MINRDTLIEDLVREHPEMVRLLMDKGVRCLACGEPVWGTIAGAA
                     AEKGFNDDRIDELVREINQRIGAQ"
     gene            4532..4966
                     /locus_tag="PKM23_08190"
     CDS             4532..4966
                     /locus_tag="PKM23_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNY91494.1"
                     /translation="MKASVKRIAGMSLAGKADSNHWVTMDADPAVGGQDGGARPLELL
                     LLGLGGCTSMDVLSILEKKRIHLDDYECLLEADRADEHPKVFTRIRIKFVFYGDAIPA
                     EAVERAIALSEEKYCSASAMLRKSAEVQVEYEIRPGRPTQAG"
     gene            5380..5961
                     /locus_tag="PKM23_08195"
     CDS             5380..5961
                     /locus_tag="PKM23_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNY91495.1"
                     /translation="MSHDNLSETELVNRAVAGDREAMGQIVTRNERLVYSTALRLLAD
                     PVEAECVLQETFLKVVQALPEFKGGSSLSTWIFRIATNYALMRLRARKKEGGSLDDVE
                     SKVSQETLEAFNRSIGNNPLHAVMNEELRQAMEEAIAALPPKFRSVFVLKDIEGFSLK
                     EIAEMLGISLAAVKTNLHRARLFLRDRLAEFSN"
     gene            6178..7029
                     /locus_tag="PKM23_08200"
     CDS             6178..7029
                     /locus_tag="PKM23_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNY91496.1"
                     /translation="MKKLFLLPTVLLAVLFSSPLLSQSRPTIAVLTLEAKNVGQETAD
                     AVADILSTELFNSQRFKVIERQAILRILEEQKLQMTGVTDMGKAVEIGKILNVEKILI
                     GSVSRLGESYIINTRLVDVKTAALEVAQNVTSKRGEEGLTGAITDLVEKLSEKVQVEG
                     SIIRIKGEVILVDLGVNHGMSENQEVRVVRLGDVVTDLGGTVIGQAEDLIGSLVLTAV
                     KGDYSEARIGVAKTPFRVGDKVRIASGRIEIEPVKPPEEKPVTKKSDKPKKNADPGQA
                     VSTPPVF"
     gene            7051..7863
                     /locus_tag="PKM23_08205"
     CDS             7051..7863
                     /locus_tag="PKM23_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91497.1"
                     /translation="MKKQLYVTVLIGLLLLAGSVLGQNATTYTVGAGVWMPGYSLIYT
                     GSQDDMEDYELGSGTYFGPYLAVNHGRWNFGASLLVGTLSPDDKEMLWEGYEAKRTDI
                     NLTVGYRVISTPSVSGNVFAGLKYVSETATADSWLWSGYDIDTWEFMQWYDEFDATTT
                     GTLYGAGASLVVPFGTSSFYAYGSMAYLLGSMDVENNLGATGINPEGYSSWDESYDLH
                     LFALTAGLGYRFPDGIGINIGYRGDLFGQDDSDESDYSQRLSGLVLNASYTF"
     gene            7972..>8527
                     /locus_tag="PKM23_08210"
     CDS             7972..>8527
                     /locus_tag="PKM23_08210"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNY91498.1"
                     /translation="MIKKADVFWTLAALTLVIAFPARADKSYTIGPVRIEAWLEQDGS
                     LRVEEERSYDFSGSFHYAFRSLPLREGVHYSDFVVLEGAQAYIEQAGDDPGSFAVRQE
                     EGLIEVRWFFSARDERRTFIFRYRLTGAVARHEDAGLFYYQFIGKDWGQRQRDVSLTL
                     HPPQEGSGGEVLHWLHGPLWARSAI"
BASE COUNT         1703 a         2517 c         2594 g         1713 t
ORIGIN      
        1 ctcattgatc acggcattga tatccatcgg ctcgaagcgc gggctggtcg gacggctcag
       61 cgagagcaga tttttaccgt gcaaggccac cttttcgagg ttggcgcaaa agagatcggc
      121 gatctcgcgg accttttccg gctccttgtc gacctccttg agtacctggg cgtgcaggag
      181 gaggccgccg acgagattgt tgagttcatg gccgatgctc ccggcgatga tgccgagggt
      241 ggcctgtttt tcggtgcgca gcagctgttc gtgggcctgc tttagatcgg tgatatcctt
      301 gtagatgagc gagacctcct cgacgctgcc gtcttcgccg gtgatcggcg ctgcggagat
      361 gaggacatgc cggggttcgc cttcgcgggt gtagcgcatc gcctcgaggc tctgcaggct
      421 ggccccctcg agcacctggg cagagaggcg cgcggcctcc tgcgagaccc cgtttttggc
      481 gatcaggctg tcgatggggc ggccgatggc ctcctcttcg gcataatgaa agatggcggt
      541 cgcacccttg ttccacgagg tgacatgggt gtccttggac atggtgataa tggcgtcggc
      601 ggcgaattcg acgatgctgc gataacgctg ctccgactcg cgcagttcgc tgatcatctt
      661 ccagatcagg ctctgcatgg tatcggagat ctcgttcagt ttctccgcgt attcctgggt
      721 tttcaattct ttcgcattca tgtgaggctc gatgaggttt tatttattat caacataaga
      781 aatattttcg acattagcaa cggctatctc gggtggctcc tgctagcggg gctttaccag
      841 ccacctctaa gtaggcacgg cggaggccgc ttgcggaaat aaaaaaaggc ggcctctgta
      901 caacagaggc cgccctgatt ttgcccggac ggctgcgcgg tcccctgccc cctagcggca
      961 gtgggaccag gccaggaccg cggcgcccag caccgcggag tcatccttga gggcggcttc
     1021 aacgatgcgg acgttgcgtg aggagatttt gaagcagttg gccaccgcca cctcgcggac
     1081 gcggtcgacg tagagggcgc cgagggcctc gaccaccccg ccgccgatga ccaccacctc
     1141 cggattgaag atattaagca tgccggcggt cgcgtagccg aggtattcgg cggatctgtt
     1201 gatcgccggc accacggcgg ggtcgccggc cagataggcc tcgagcaggc gccggctgcg
     1261 gatcggcgca tcggggttct cgaccccctc gaagaggggg gagggggtgc ccgccgcgat
     1321 cgcggcgcgg atctccctct cgatggagag gcggctggcg aaggcctcga ggcagccgtt
     1381 attgccgcag ccgcagcgtg ggccatgggg atcgaggatg acatggccga tttcgcccgc
     1441 attctcgtta aagccgtgca ggagcttgcc gccgatgatg atgcccccgc cgatgccggt
     1501 gccgacaaaa aaaccgatca catcccgggc ctccctggcg gcgccatagg cgtgctcccc
     1561 cagaataccg acgttgcaat cattgtcgac atagactggc tttttcagca gccgtttgag
     1621 ctcctttttg aggggggcct ccttccaggc caggttcggg ctctcgatga tcatcccctt
     1681 cttcatgtcc agggggccgg gcgagcccac acccaccgcc cgtataccat cccaggagac
     1741 cccggcgttg accacggcct ggcgggcaca atccgccatg cggctgaccg ttgcaggaaa
     1801 tccgagctcg gacttggtct ttttgcgctc gctgccgagg atcgtgccct tgcgatcgat
     1861 gacggccgca tagatcttgg tgccccccag atcgatgccg acaacccatt ccttcttcga
     1921 tttagccata gttttcagcc gtctcctttt ggccctccca gggcggcgca taaatgccgc
     1981 tgtctgggat cagtcaatgc gataagtgat gccgggttcg agaacctcgg cgatgctcaa
     2041 tccggcgaac cccgtgctga tcgatagttc aaaaagcccg aatttggcgg gattgcccgg
     2101 tttgaggaaa aagctgtagt ttctgcggat gctctccagg accatcttct ccggagagat
     2161 gctgctgtaa taaaggacca tcaggttgat ggccccctgg ctgcgctgct ggtcctcgct
     2221 gcgcatcttg ccccacaaac cctggttgtt ctgttgcgta atcagccagt tgatgctgcg
     2281 gttgatcgag gcggcgatct cctcctgcat ggccgtactg ggggtgcgac gatagaggcc
     2341 gatgaggcca tcgctgacgt agctgagggt gttgatcggc cactcctcgt ggatgcggtt
     2401 ttcgatggtg taagggatgt ggccgtcagg ggcccgctgc agcagcagcc acttgccggc
     2461 cgcctcggcg gtgcggatgt cctccggcgc gcgggtgagg ccgtaaaggg ccgagaagaa
     2521 gccgcagccg gtcacggcgg tggcgatggt gtagggggcg accgcaagct ggcccttgta
     2581 atagccggtg ccgagggcgc cgcggttggg tccggtctcg atcacccagc cgggcgagcc
     2641 gccgcgcccc ttgccctggg gatcctcggc gcagccgtgg cggacaaaat tggcatagcg
     2701 ccgcagggcg cgcagatagg cggcgcgccg gggagcgctg gcaaaacgga cggccccggc
     2761 caggccggtg gccgaggtgc cggcatcacc gaggtagatg ttggcgtcgg gcgccatgtc
     2821 accccaccag cccaccgtat cccctttcgc cgagagggtg acctgctgca gttcaaccag
     2881 gcggtcgaac caggcgaggg cctcctcccg gtagcgttct tcgccgcaga tctgataacc
     2941 ggccaggagg acgcgggcga ggttgctgtt gataaagatc gaggtccgcc gtttttcggc
     3001 gatcttgatc tcgccggtgc gcagctcgac cgcaatcgcc cagtcgcaga tctcgcgcag
     3061 cagctgcgtc agctcctgcc gcgttttctc atcggtgacg accggacgcg tttgtgcgga
     3121 gcctttcgcc gctatcagcg cagccgccag gagacccagc atcatgatcc agctcttcct
     3181 catgcttgag ccctttccag agtccatttc cgtcccctcc ctcctgaccg gcgcagtttg
     3241 ccgccgacgg cttgcaggga tataatgcaa aaagtttact gaaataagga cacaatgtca
     3301 agagaatagt aagaaaacag gctgcccccc tctaatctgg cgcccacccg gcaccgtgca
     3361 ccccctctaa tctggcgccc acccggcacc gtgcgccccc tctaatcttg cactacccga
     3421 cacctgcgcc gcttgccccc gcacctcctt gctgtttcca gcatactcgc ggcctgtgcc
     3481 ctggatgatg aaaccttttc ccgcctgcat catctaaatg taaaaccggg aaaaaggaat
     3541 gagtgctgag aaaaaggcct tgtacattat cagggaaaac tgcggcgatg tcgcacagcg
     3601 catacgcgcc tgtcggtcgc ggcagatcgc ccttcagctt cggcagtgct tgtgccgaga
     3661 actggagaac agctgcacaa gcccggagat gaaaaggctg ctgcaaaacc atatcaatga
     3721 actcatcaca gatacatttg acgaaaatgg catgaaccgc tatctggagg catgatgaaa
     3781 agaggaatga tctttatcgc aaccgccctg atcctcgcca ccggcgtgca gacgggctgc
     3841 accaaacaga aggaaaaggc caacgctgct gtgcctgtga aagaggccgc atcatccggc
     3901 ctcaagagtg cccccgcctt tgaactggcg ggcacggatg gcaagaccat caaactgtcc
     3961 gattacaagg gcaaggtcgt gatcgttgat ttctgggata cctggtgccc cccatgccgc
     4021 atggagatcc cccatttcat tgagctccag aaccagtaca aatccaaagg ctttgagatg
     4081 atcggcgtcg tcctcggccg cgagggcaag gagaaggtgc tcgacttcat caaaagcaac
     4141 aagatcaatt acgtcaacgc ctacgcgacc gaggccttcc tcaacgccta cgggccgatc
     4201 gagggcatcc ccaccacctt cgttatcgac cagcagggac ggatctacaa gagctataca
     4261 ggctatcgcg acaaggctgt tttcgaaaac gacatcaaag ccctgctcaa actctagaac
     4321 aagtgaccat gataaaccgc gataccctga tcgaggatct ggtgcgggag cacccggaga
     4381 tggtgcgcct gctcatggac aagggggtgc gctgcctcgc ctgcggtgag ccggtctggg
     4441 gaaccatcgc cggcgcggcc gcagaaaagg gcttcaatga tgaccggatc gatgaactgg
     4501 tgcgggaaat caatcaaagg attggagcgc aatgaaagca tccgtcaaac gcatcgcagg
     4561 aatgagtctg gccggcaagg cggattccaa ccactgggtt accatggacg ccgatccggc
     4621 ggtcggcggg caggatggcg gggcgcgccc gctcgaactg ctcctgctcg gtttgggcgg
     4681 ctgcacctcc atggatgtgc tctcgatcct cgagaaaaag cgcatccacc tcgatgacta
     4741 tgaatgtttg ctggaggcgg atcgcgccga cgagcacccc aaggttttca cccgcatccg
     4801 catcaagttc gtcttttacg gcgatgcgat cccggcggag gcggtcgagc gcgccattgc
     4861 cctctcagag gagaaatact gttctgcttc ggccatgctg cgcaagagcg ctgaggtgca
     4921 ggtcgaatat gaaatccggc cgggccggcc gacgcaggcg ggatgagcta tcctcccatg
     4981 ccgctctgat cggtcttctt tcccgctcct catcatcctt cagcctgacg gcgccgccgg
     5041 ttcgaacgaa ccggcggcgc ttctctttaa taaatcaggc taccccccga aatctcgttc
     5101 ccacccaaag ataattaatt aaaaaaataa atgttatctg ccataaatac cttgtaaaat
     5161 aaatggcctc atgaagaggg cacaggctgt gagtaggctg gaaatggtat ggagggattg
     5221 gagtacgcgg cgccaggcgt caggtgggag ccagatttcg ggggtaccct ctttaataaa
     5281 ttccttgcta ctctatatta atatgtttaa gttaaattct gcagcagccg tcctccggcg
     5341 gccggaaaac tgaacagaag caaaacgggc agcagcggaa tgagccacga caatctgagc
     5401 gaaacagagc tggtcaatcg ggcagtggcg ggcgaccggg aggcgatggg ccagatcgtg
     5461 acccggaacg aacgcctggt ctacagcaca gccctgcggc ttctcgccga cccggtcgag
     5521 gccgagtgtg tgctgcagga gacctttctc aaggtggtgc aggccctgcc cgagttcaag
     5581 ggcggatcct cgctctcgac ctggatcttc cgcatcgcca ccaactatgc cctgatgcgg
     5641 ctgcgcgcac gcaagaaaga gggcggatcg ctggatgatg tggagagcaa ggtgagccag
     5701 gagaccctcg aggctttcaa tcgctcgatc ggcaacaatc ccctgcatgc cgtgatgaat
     5761 gaggagctgc gccaggccat ggaggaggcg atcgccgccc tgccgcccaa attccgttcc
     5821 gttttcgtcc tcaaggacat cgaaggcttt tccctcaaag agatcgcaga gatgctgggg
     5881 atcagcctgg ctgcggtcaa gaccaacctg catcgcgccc gcctctttct ccgcgaccgc
     5941 ctggctgaat tcagcaacta gccccggggt atcagaccgc tgtccgcatc atgcaggtcc
     6001 ggtctttgcc cgggcgccgc gatgaatccg cgggcccctg caccaccgca tccgcgcatc
     6061 ggaacaagcc tggcggtggc gctgttaata cataaaatag cgcttgacaa ataatcgctt
     6121 cgtgagtaca ttgaattatc agcaggttga atcatcatcg tgcatccgag gggttacatg
     6181 aaaaagctct tcctgttacc aaccgtactg ctcgcggttc ttttctcctc cccactcctc
     6241 tcccagagca gacccactat cgccgtcctc accctcgagg ccaagaatgt cggtcaggaa
     6301 accgctgatg cggtggctga catcctcagc accgagcttt tcaactccca gcgtttcaag
     6361 gtcatcgagc gccaggcgat cctgcgcatc cttgaggagc aaaagctgca gatgacgggc
     6421 gtgaccgata tgggcaaggc tgtggagatc ggcaagatcc tcaacgtcga aaagatcctc
     6481 atcggcagcg tcagccggct cggtgagagc tatatcatca acacccgcct ggtcgatgtc
     6541 aagaccgccg cgctggaggt ggcccagaat gtcaccagca agcgcggcga ggaagggctg
     6601 accggggcga tcaccgatct ggtggaaaaa ctctcggaaa aggtccaggt cgagggatcg
     6661 atcatccgca tcaagggcga ggtcatcctc gtggatcttg gcgtcaacca cggcatgtcg
     6721 gaaaaccagg aggtaagggt cgtgcgtctg ggggatgtgg tcaccgatct gggcgggacg
     6781 gtgatcggcc aggcggagga tttgatcggc agccttgttc tgaccgctgt caagggggat
     6841 tattcggagg cgcgtatcgg tgtggccaaa acaccctttc gggtaggtga caaggtgcgg
     6901 atcgccagcg ggcggatcga gatcgagccc gtcaagcccc ctgaggagaa accggtcact
     6961 aaaaagagtg acaaaccgaa gaaaaacgcg gatccggggc aggcggtcag cacccccccg
     7021 gtcttttaat cccgaagagg agggagcggt atgaaaaagc aactctatgt gacggtcctg
     7081 atcggactct tgctgctggc gggcagcgtt ctcgggcaga atgcgaccac ctataccgtc
     7141 ggcgccgggg tgtggatgcc aggctactcg ctcatctata cgggaagtca ggatgatatg
     7201 gaggactatg agctcggcag cggcacctat ttcggtccct atctggccgt caaccacggg
     7261 cgctggaatt tcggggcttc gctgctggtc gggacgctca gcccggatga caaggagatg
     7321 ctctgggagg gctacgaagc caaacgcacc gacatcaacc tgacggtggg ctatagggtg
     7381 atcagcaccc ccagtgtgag cggcaatgtt tttgccggcc tcaagtatgt gagcgagacg
     7441 gcgaccgctg attcctggct gtggtcgggt tatgacatcg acacctggga gttcatgcag
     7501 tggtatgacg agttcgacgc caccaccaca ggcacacttt acggcgccgg ggcctcgctg
     7561 gttgtcccct ttggcacctc cagcttctat gcctacggct ccatggccta tctcctcggc
     7621 agcatggacg ttgaaaacaa cctcggcgcc accggaatta atcccgaagg ctactcctcc
     7681 tgggatgagt cctatgatct tcatcttttc gccctcaccg ccggcctcgg ctatcgtttc
     7741 ccggacggca tcggcatcaa tatcggctat cgcggggatc tctttggtca ggatgattcc
     7801 gatgagtccg attattccca gcggctttcg ggactggtgc tcaacgcctc ctacaccttt
     7861 tagtcccgca gtccagggaa agggcatcag gaagcctgcc tgcgggcagg cttttctgta
     7921 tcagcgggag atgaggcggt tttcactgtt aacaattttc cggagtgtaa gatgataaaa
     7981 aaggccgacg tgttctggac gcttgcagcc ctcaccctgg tgattgcgtt tccggcccgg
     8041 gcggacaagt cctataccat cgggccggtc cggatcgagg cctggcttga gcaggacgga
     8101 tccctgcggg tggaggagga gcggagctac gatttcagcg gcagcttcca ttatgccttt
     8161 cgctccctgc ccctgcgcga gggggtgcac tacagcgatt tcgtcgttct cgagggtgct
     8221 caggcctata tcgagcaagc gggcgatgat cccggctcct ttgcggtgag gcaggaggag
     8281 ggcttgatcg aggtgcgctg gtttttttca gcccgggatg agcgccgcac ctttatcttc
     8341 cgttaccggc tgaccggggc ggtcgcccgc cacgaggatg ccggactttt ttattaccag
     8401 ttcatcggca aggactgggg ccagaggcag agggatgtga gcctgaccct tcatcccccg
     8461 caggagggca gcgggggtga ggtgctgcac tggctgcatg gcccgctctg ggcccgttcg
     8521 gccatcc
//
LOCUS       DAOPWU010000133         8496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8338_length_8496_cov_5.348063, whole genome shotgun sequence.
ACCESSION   DAOPWU010000133 DAOPWU010000000
VERSION     DAOPWU010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8338_length_8496_cov_5.348063"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1166
                     /locus_tag="PKM23_08215"
     CDS             90..1166
                     /locus_tag="PKM23_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNY91499.1"
                     /translation="MTILDRYLLRKFAGVLVFALLAFICIFLIVDGIEKLDTFIDKEV
                     PAGIVIRLYLYYIPYIITLTLPVAMLLASLFSVGTMAKQNEIIAQIASGLSLYRILAP
                     LLIFSLLISLGAFWFGETVVPRASAARSGIVDEHIEKQREVWRKRVNNVYARDAQERR
                     ISMRWFDTVQNIGHKINIRRFEGLDLELHIYANRMEWKDNQWVLYEGVERTFSGEGET
                     VRTFSSLILEDENLTPADFGKVLKKPEEMAYAELRSFIKEVERNGGDPDHWLVDLYLK
                     IAIPFANFIIVLFGAPLSSPKRRTGTATGFGISLAVCFIYFGIVKTTQSFGQNGILPP
                     LVAAWSANVLFLAAGLYVLFKAPK"
     gene            complement(1203..1880)
                     /locus_tag="PKM23_08220"
     CDS             complement(1203..1880)
                     /locus_tag="PKM23_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91500.1"
                     /translation="MDNGIIEEAPVERIRLSSRQSRELAPALYQALGRQAPSFLEAQG
                     DLRLLRGCKLALFCSVRSPGSILSKSFDLANGLRQNLVTVMSGFHSPIEKEWFALLQR
                     STTPMISCPARSLEKRRLSPEERSLMAQGRYLLLNFGISEGRITRETAFQRNLCMAAA
                     ADLVFVAYAAPGGKTEYLCEQVRKWGKPVWTFADAATAHLQKLGVRSIRVGAFISGLA
                     SPGASEP"
     gene            complement(1976..3172)
                     /gene="mutY"
                     /locus_tag="PKM23_08225"
     CDS             complement(1976..3172)
                     /gene="mutY"
                     /locus_tag="PKM23_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNY91501.1"
                     /translation="MSREMRPQQERKKSAGGERADAVHEPDARWQPAAPAAAEWLQRC
                     RAALLAWFDASARVLPWREDRDPYRIWISEIMLQQTQVRQVLPYYERFLARFPDIPSL
                     AAAPLDAVLKAWEGMGYYARARNLHRAAGEIMARYGGRFPQEREAVLGLPGVGVYTAA
                     AILSIAFGRPEVVVDGNVVRVIARLTAMAEEARGAGRRRIEGMAAAFFDPGRPGDFNE
                     AMMELGAVICTPRAPVCALCPVAACCAALKEGAPERYPVKSPRRAKPHHQIAAALIWQ
                     EGRLLIARRPEKGLLGGLWEFPGGKQENGESLEQAAVREAAEELGVEIEVVNRFISID
                     HAYTHFSITLHLFHCRYLGGEPACKACSDWRWITPEQLREYAFPRANLRAIEALLEEK
                     IAVPKI"
     gene            complement(3169..3735)
                     /locus_tag="PKM23_08230"
     CDS             complement(3169..3735)
                     /locus_tag="PKM23_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNY91502.1"
                     /translation="MAELSREGGLKQVILARRSVRRFDGRPVERELLRSCVDAARLAP
                     SAENVQPWRFLVVDDPTLKARLAEGAFSGIYRVTSWAERAPVLVVLFAELDILANRLG
                     KRVTGIDYYLLDLGIAGEHFCLQAAELGLGTCWIGWFSSDGVRKALGTPRRLRPAALF
                     AVGYSAHERMPEKKRRVLDEICWFNEIG"
     gene            3844..3916
                     /locus_tag="PKM23_08235"
     tRNA            3844..3916
                     /locus_tag="PKM23_08235"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3877..3879,aa:Lys,seq:ctt)
     gene            3984..4877
                     /locus_tag="PKM23_08240"
     CDS             3984..4877
                     /locus_tag="PKM23_08240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY91503.1"
                     /translation="MVTESKPLVSVIIASYNQKHYIFDALASVFDQQCDFPFEVIVAD
                     SSGDDTPALVQSRFPKTRVIALKERAYPGTARNAGLEIALGEYFAFTDTDCLVDRRWL
                     QNLVAAHRKGYPVVGGIVKNGTPWSLFGTLDYLMECSDLLTPYATTAKDHFGTGNVSF
                     SRAIYDEYGPFANQVKGSDNIYFRKIHGRGQALYWEPKAVIWHRNRTSLKKILRNQYE
                     LGVGSAQNRKKNQVSGRIFVRYPVLIPLIPVVRILAIGQRLLRYSPLNFLKYLVMLPL
                     VLLCLIWYTAGFWHGYRLPQG"
     gene            complement(4874..6613)
                     /locus_tag="PKM23_08245"
     CDS             complement(4874..6613)
                     /locus_tag="PKM23_08245"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNY91504.1"
                     /translation="MTEPCFRMALVQFNPVVGDLPGNRGRIIDRMAEAAEAGCDLIAF
                     PELALTGYPPEDLLLRRQFVADQREQLQAIAAASGDCLCVVGCVDELQGRLYNAAALL
                     HRGEMIGAYHKVHLPNYSVFDEERYFEAGRRPLVLDFGALRIGVSICEDIWIEESVVE
                     AEALLGGADLLLNISASPFHSHKGDERLELMQARARRTGSIVAYLNLIGGQDELVFDG
                     RSLVVAADGRLLAAGGGFQEDFVVVDLPLEEFATLRRARARLATPAAALDPPQLLAVP
                     WSPRTRPEGRPEPRAIVPVKNEAEEIYQALLLGLRDYTRKNGFAKVTLGLSGGIDSAL
                     VTVLAVDALGADQVVAVTMPSRFSSSGSVTDSEELARNLGIRLHTLPIAPALTAYEEM
                     LAELFAGRAPDVAEENLQARIRGNLLMALSNKFGWMVLVTGNKSEVSVGYCTIYGDMA
                     GGFSPLKDVYKTAVYALCEYRNKLAGYDLIPRAIISKPPSAELRPDQTDQQSLPPYDL
                     LDAVLELYVEKEMGAAEIIAQGFDGEMVRRVARLVDFAEYKRRQGAPGIKITPRAFGK
                     DRRMPITNHYREL"
     gene            6786..7793
                     /gene="rfbB"
                     /locus_tag="PKM23_08250"
     CDS             6786..7793
                     /gene="rfbB"
                     /locus_tag="PKM23_08250"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006284889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNY91505.1"
                     /translation="MKQYLVTGGAGFIGSNFIHYLLAKYGSEVRIVNVDKLTYAGNLD
                     NLKDVANAPNYRFVHGDICDARLMDELVPPADVVVNFAAESHVDRSIGAPDDFIKTDI
                     YGAFVLLEAARKYGVKKFIQISTDEVYGSILDGSFKESDRLEPSSPYSASKAGADRLA
                     FSYFVTYGLPVVITRCSNNYGPCQFPEKLIPLFVTNALEDKSLPIYGDGKNVRDWIYV
                     MDHCDAIDFLISHGDDGQVYNIGSGNERTNLDITGIILRELGKPESLMTWVPDRLGHD
                     RRYSVDTAKIRALGWKPGHTFEEAMTSTIRWYRENKWWWEKLKTGEYLEFYKQNYKRD
                     L"
     gene            7813..>8496
                     /locus_tag="PKM23_08255"
     CDS             7813..>8496
                     /locus_tag="PKM23_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNY91506.1"
                     /translation="MRTILNVVGARPNFMKIAPIHHRMLERGDVRPILVHTGQHYDQK
                     MSHAFFTELGMPEPDQFLGVGSGTHAEQTGAVMIAIERLLLDLKPDLVVVVGDVNSTL
                     AAALAAAKLHVPIAHVEAGLRSRDRTMPEEVNRILTDALAEWLFITEQSGWDHLRAEG
                     VAAHKMHFVGNVMIDSLREHLDKARACPVMEELGLSPREYALVTLHRPSNVDNPLLLD
                     TILTAIGKLQ"
BASE COUNT         1651 a         2876 c         2411 g         1558 t
ORIGIN      
        1 agaaactttt tcaatcgcat ccgcagcctc gccggcgtag ccgcccctga agagaacggc
       61 aaaacctgaa cagaaacctg acgatcatta tgaccatact cgaccgctat ctgctgcgta
      121 aattcgccgg cgttctcgtc ttcgccctcc tggcctttat ctgcatcttt ctcatcgtcg
      181 atgggattga aaagctcgac acctttatcg acaaggaggt ccccgccggc atcgtcatca
      241 ggctctatct ctattatatc ccctatatca tcaccctgac cctgccggtc gccatgctgc
      301 tggccagcct cttcagcgtt ggcaccatgg ccaaacagaa cgagatcatc gcacagatcg
      361 cctccggact ctccctctac cgcatcctcg cccccctcct tatcttcagc ctgctcatca
      421 gcctcggcgc attctggttc ggcgagaccg tagtgccccg ggctagcgcc gcgcgcagcg
      481 ggatcgtcga cgagcacatt gaaaaacagc gcgaggtgtg gcgcaaaagg gtgaacaacg
      541 tctatgcccg ggatgcccag gaacgccgca tcagcatgcg ctggttcgac actgtacaga
      601 atatcggcca caagatcaac atccgccgct tcgaggggct ggatctcgag ctacacatct
      661 atgccaaccg catggaatgg aaggacaatc agtgggtgct ctatgagggg gtggaacgga
      721 ccttctctgg ggagggggag acagtgcgga ccttctccag cctgatcctg gaggatgaga
      781 atctcacgcc cgccgatttc ggcaaggtgc ttaaaaaacc ggaagagatg gcgtacgcgg
      841 aactgcgcag tttcatcaag gaggtggagc gcaacggcgg cgaccccgac cactggctgg
      901 tcgatctcta tctcaagatc gccataccct tcgccaattt catcatcgtc ctcttcggcg
      961 ccccgctctc ttcacccaag cggcgcaccg gcaccgcaac cggcttcgga atcagcctcg
     1021 cggtctgctt catctatttc gggattgtca agaccaccca atccttcgga cagaacggca
     1081 tcctcccccc gctggtggcc gcctggtccg ccaacgtcct cttcctggcc gccggccttt
     1141 atgtgctgtt caaagccccg aaataatccc ttccaaccat cactcccggc tgtacaggag
     1201 cctcagggct cggacgcgcc gggactggcc agcccgctga tgaaagcacc gaccctgatg
     1261 ctgcgcaccc ccagcttctg aagatgggca gtcgcggcgt cggcgaaggt ccagaccggt
     1321 ttgccccatt tgcgcacctg ctcgcagaga tactctgtct ttccgccggg ggccgcatag
     1381 gcgacgaaga ccagatccgc cgccgcggcc atgcagaggt tgcgctgaaa ggcggtctcg
     1441 cgggtgatcc gcccttcgga gatcccgaaa ttgagcagca gatagcggcc ctgcgccatc
     1501 agggagcgct cttccggcga aagccgccgc ttttccaggg agcgcgccgg gcagctgatc
     1561 atcggcgtgg tgctgcgctg gagcagcgcg aaccactcct tctctatcgg ggaatgaaaa
     1621 ccgctcatca ctgtgaccag gttctggcgc aggccgttgg ccagatcgaa ggacttgctg
     1681 aggatgctgc cgggagagcg gaccgagcaa aagagggcca gcttgcagcc ccgcagcagt
     1741 cggagatccc cctgggcttc cagaaaggag ggtgcctggc ggccgagggc ctgatagagg
     1801 gccggcgcca gctcccgact ttggcggctg gagagacgga tgcgctccac cggcgcctcc
     1861 tcgatgatgc cattatccat acgattttcc ccttggtctg ctgcggtaaa acggctatcc
     1921 ccgggcaatg gtttgctgct gattaaatat aaggcatttt cagccgtgca tcaattcaaa
     1981 tcttcggtac ggcgattttt tcctcgagaa gcgcctcgat ggcgcggagg ttggcgcgtg
     2041 gaaaggcata ctcccgcagc tgctccggtg tgatccagcg ccagtcgctg caggccttgc
     2101 aggccggttc gcccccaagg tagcggcagt ggaaaagatg cagggtgatg ctgaaatggg
     2161 tgtaggcgtg atcgatgctg ataaacctgt tgacgacctc gatctccacc cccagctcct
     2221 cggccgcctc gcggaccgca gcctgctcca gactctcccc gttctcctgc ttgcccccgg
     2281 ggaattccca caatcctccc agcagtccct tctccgggcg gcgggcgatg agcagccgcc
     2341 cctcctgcca gatcagggcg gcggcgattt gatggtgcgg tttggcacgg cgcggggatt
     2401 tgaccggata gcgttccggg gccccctctt tgagggctgc gcagcaggcg gcgaccgggc
     2461 agagcgcgca cacgggcgcc ctcggggtgc agatcaccgc ccccagttcc atcatcgcct
     2521 cgttgaaatc acccggccgg ccgggatcaa aaaaagctgc ggccataccc tcgatccgcc
     2581 tccggcctgc cccccgggcc tcctccgcca tcgccgtcag ccgcgcgatc accctcacca
     2641 cattgccgtc gaccaccacc tcggggcggc cgaaggcgat gctcaatatc gccgccgccg
     2701 tatagacccc gacgcccggc agccccagca cggcctcccg ctcctgcgga aagcgcccac
     2761 cgtaccgggc catgatctcc cccgccgccc ggtgcagatt gcgcgccctt gcataatagc
     2821 ccatgccctc ccaggccttg agcaccgcat ccaggggcgc ggccgccagg ctcgggatgt
     2881 cgggaaagcg ggcgagaaaa cgctcataat agggcagcac ctggcggacc tgggtttgct
     2941 gaagcatgat ctcggagatc cagatccggt agggatcgcg gtcttcccgc cagggcagca
     3001 cccgcgccga ggcgtcgaac cacgccagca gcgccgcccg gcagcgctgc agccactccg
     3061 cagcggccgg agcggccggc tgccatcggg cgtcgggctc atgtactgca tccgcccgct
     3121 ctcctcctgc gctctttttt ctctcctgct gcggcctcat ttccctgctc acccgatctc
     3181 gttgaaccag cagatctcat ccagtacgcg ccgttttttt tcgggcatgc gctcatgagc
     3241 ggagtagccg accgcgaaca gggccgccgg ccgcaagcgg cgcggcgttc cgagggcctt
     3301 gcgcaccccg tcggagctga accagccgat ccagcaggtg cccagcccca gttccgccgc
     3361 ctgcaggcaa aagtgctcgc cggcgatgcc gagatcaagc agataatagt cgatgcccgt
     3421 cacccgcttc cccaaccggt tggcgaggat atcgagttcg gcgaaaagga ccaccagcac
     3481 cggggccctt tcggcccagc tggtgacccg gtaaatcccg gaaaaggccc cctctgccag
     3541 acgggccttc aaagtgggat cgtcgacgac cagaaagcgc cacggctgca cattctcggc
     3601 cgagggggcc agacgcgccg catcgacaca gctccgcagc agctcccgct ctaccggccg
     3661 gccgtcgaaa cgacgcacac tccgccgcgc cagaataacc tgcttcaatc cgccctctcg
     3721 gctcagctcc gccatgcgcc tgctctccct tacttgatga taaattctcc ggcccagata
     3781 attttttact tgataaaaag gggaatttgt gatacattat atcctgcaat tgcagtaaaa
     3841 aatgcaccca tagctcaact ggcagagcaa ctgactctta atcagtaggt tcaaggttcg
     3901 attccttgtg ggtgcacata tcaggtgagg gtattcagca gaataccctc tttttttgcc
     3961 tcacccgttc cgcaggtcca tgcatggtga cagaaagcaa gcccctggtt tcggtgatca
     4021 tcgcctccta caaccagaag cactacatct ttgatgccct cgcctcggtc ttcgaccagc
     4081 agtgcgattt tcccttcgag gtgattgtcg ccgactcgag cggagatgac acccccgccc
     4141 tggtgcagag ccgctttccc aaaacccgcg tcatcgccct caaggagcgc gcctatcccg
     4201 gcaccgcgcg caatgccggg ctcgagatcg ccctcgggga gtactttgcc ttcaccgaca
     4261 ccgactgtct ggtcgaccgc cgctggctgc agaacctcgt cgctgcccac cgcaagggct
     4321 atccggtggt gggtggaatc gtcaaaaacg gcaccccctg gtccctcttc ggcaccctcg
     4381 attatctcat ggagtgcagc gacctgctca ccccctacgc caccacagcc aaggaccatt
     4441 tcggcaccgg caacgtctcc ttcagccgcg ctatttacga cgagtacggc cccttcgcca
     4501 atcaggtcaa aggcagcgat aatatctatt tccgcaaaat ccacggccgc ggccaggcgc
     4561 tttactggga gcccaaggcc gtcatctggc accgcaaccg caccagcctg aagaaaatcc
     4621 tgcgcaacca gtacgagctc ggggtcggct cggcgcagaa ccgcaagaag aatcaggtct
     4681 ccggcaggat tttcgtccgc tatcccgtcc tcatccccct catcccggtg gtgcgcatcc
     4741 tggccatcgg ccagcgcctg ctgcgttaca gtcccctcaa ttttctcaag tatctggtca
     4801 tgctgcccct ggtgctgctc tgcctgatct ggtataccgc cggattctgg cacggctacc
     4861 gcctgccaca gggttaaagc tcccggtaat ggttggtgat gggcatgcgc cggtccttgc
     4921 cgaaggcgcg cggggtgatc ttgatgcccg gtgcgccctg gcggcgcttg tactcggcga
     4981 aatcaaccag cctcgccacc ctgcgcacca tctctccatc gaaaccctgc gcgatgatct
     5041 ccgcggcccc catctccttt tcgacataca gctcgagtac ggcatcgagc agatcatagg
     5101 gaggcaggct ctgctgatcg gtctgatccg gacgcagctc cgcggaaggg ggcttggaga
     5161 tgatggcccg cgggatgaga tcgtatccgg ccagtttgtt gcggtattcg cagagggcgt
     5221 agactgccgt cttgtagaca tccttgaggg gggaaaagcc tccggccatg tcgccataaa
     5281 ttgtgcagta gccgacgctg acctcgctct tgttgccggt gaccagaacc atccagccga
     5341 atttgttgga gagggccatg agcaggttgc cgcggatgcg cgcctgcaga ttctcctcgg
     5401 cgacgtcggg cgcccgcccc gcgaacagct ccgccagcat ctcctcatag gccgtcaggg
     5461 ccggggcgat cggcagggta tgcagccgga tgccgaggtt gcgcgccagc tcttcagagt
     5521 cggtgacgct gcctgaagat gaaaagcggg agggcatggt caccgccacc acctgatcgg
     5581 cgccgagggc gtcgaccgcc agcacggtga ccagcgcgga atcgatgccg ccgctcaagc
     5641 ccagggttac cttggcaaag ccgttcttgc gggtatagtc gcgcagtccg aggagaaggg
     5701 cctgatagat ctcctcggcc tcattcttga ccgggacaat ggcgcgcggt tccgggcggc
     5761 cttccggacg ggtccggggg ctccagggca cagccaggag ttggggggga tcgagagcgg
     5821 cagcgggtgt cgccagacgg gcgcgggcgc ggcgcagcgt ggcgaactct tcaaggggca
     5881 gatcgacgac gacgaaatcc tcctgaaaac caccccctgc ggcgagcagg cggccatccg
     5941 cggccaccac cagactgcgg ccgtcgaaga ccagctcgtc ctgaccgccg atcagattga
     6001 gataggcgac aatgctgccg gtgcggcgcg cccgcgcctg catcagctcc agccgttcat
     6061 cccccttgtg gctgtgaaaa ggcgaagcgg aaatattgag caggagatcg gctcccccga
     6121 gcagggcttc agcctcgacc acactctcct cgatccagat gtcctcgcag atgctcaccc
     6181 cgatgcgcag ggcgccaaag tcgaggacca gggggcgccg gcccgcctcg aagtagcgtt
     6241 cctcgtcgaa gacgctgtaa ttgggcaaat gcaccttgtg ataggcgccg atcatctcgc
     6301 cgcgatggag gagggcggcg gcgttataga gccgcccctg cagctcgtcg acgcagccga
     6361 caacgcacaa acagtcaccg ctcgcagcag cgatagcctg gagctgctcg cgctggtcag
     6421 caacgaactg ccgccgcagc agcagatcct cggggggata tccggtcagg gccagctcgg
     6481 ggaaggcgat cagatcgcag cctgcctcgg ctgcctcggc catccggtcg atgatcctgc
     6541 cgcggttgcc cggcagatcg ccgacaacgg gattgaactg caccagtgcc attcgaaagc
     6601 agggttcggt catggggtgg tttccttttt ccgcaaagcg gcccccttcg cggtactatg
     6661 attaggtcca aacgcggctg ttcataaaaa ataatggatt gacttttaac agccgggttt
     6721 gtacattgaa aattaggaaa aaaaagccaa ttcagcaacc ccctttattg acgacaggca
     6781 ggatcatgaa acagtatctc gtcaccggcg gcgccggttt tatcggcagc aatttcatcc
     6841 actacctcct cgccaagtac ggcagtgagg tccggattgt caacgtcgac aaactgacct
     6901 atgcgggcaa tctcgacaac ctcaaggatg tcgcgaacgc ccccaattac cgcttcgttc
     6961 atggcgatat ctgcgacgcc cggctcatgg acgaactcgt ccccccggcc gacgtcgtgg
     7021 tcaatttcgc cgccgagagc cacgtcgacc gctcgatcgg cgcccccgat gatttcatca
     7081 agaccgacat ctatggcgcc tttgtcctcc tcgaagccgc ccgcaaatac ggcgtgaaaa
     7141 aattcattca gatcagcacc gatgaggtct acggcagcat cctcgacggc tccttcaagg
     7201 agtccgaccg gctcgaaccc tcgagcccct actccgcctc caaggcggga gccgaccggc
     7261 tcgccttctc ctatttcgtc acctacggtc tgccggtggt catcacccgc tgctccaaca
     7321 attacggccc ctgccagttc ccggaaaaac tcatccctct cttcgtcacc aacgccctcg
     7381 aggacaagag cctgcccatt tacggcgacg gcaagaacgt gcgcgactgg atctatgtga
     7441 tggaccactg cgacgccatc gattttctca tcagccacgg cgacgacggc caggtctaca
     7501 acatcggctc cggcaacgag cgcaccaacc tcgacatcac cggcatcatc ctgcgcgagc
     7561 tgggcaagcc cgaaagcctg atgacctggg tccccgaccg cctcggccac gaccgccgct
     7621 actcggtcga caccgctaaa atccgcgccc tgggctggaa gcccggccac accttcgagg
     7681 aggcgatgac ctccaccatc cgctggtacc gggagaacaa gtggtggtgg gaaaagctca
     7741 agaccgggga gtacctcgag ttctacaagc aaaactacaa acgcgatctc taggccgggg
     7801 cccgaacact acatgcgcac catcctgaac gtcgtcgggg ccaggcccaa tttcatgaaa
     7861 attgctccca tccaccaccg catgctcgaa cgcggggatg tgcgccccat cctggtccac
     7921 accggtcagc attacgacca gaagatgtcg cacgcctttt tcactgaact cggcatgccc
     7981 gaacccgacc agttcctcgg cgtcggctcc ggcacccacg ccgaacagac cggggcggtg
     8041 atgatcgcca tcgagcgcct gctcctcgat ctcaagcccg atctggtggt ggtggtcggc
     8101 gacgtcaact ccaccctcgc cgccgccctc gccgcggcca aactgcacgt ccccatcgcc
     8161 catgtcgagg ccggcctgcg cagccgggac cgcaccatgc ccgaagaggt caaccgtatc
     8221 ctcaccgatg ccctggccga gtggctcttt atcaccgaac agagcggctg ggaccatttg
     8281 cgcgcagagg gggtggcggc ccacaaaatg cactttgtcg gcaatgtcat gatcgactcc
     8341 ctgcgtgaac atctcgacaa ggcccgcgcc tgcccggtca tggaggagct ggggctctcg
     8401 ccccgggagt acgccctcgt cactttgcac aggccctcga atgtcgacaa ccccctcctc
     8461 ctcgacacca tcctcaccgc catcggcaag ctgcag
//
LOCUS       DAOPWU010000134         8451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8397_length_8451_cov_4.707480, whole genome shotgun sequence.
ACCESSION   DAOPWU010000134 DAOPWU010000000
VERSION     DAOPWU010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8397_length_8451_cov_4.707480"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1298)
                     /locus_tag="PKM23_08260"
     CDS             complement(114..1298)
                     /locus_tag="PKM23_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91507.1"
                     /translation="MKKGRSAKTNRQKSSPSLEKRRLAKADPVSLLGDRPVRELWNEH
                     VNRKNGKKSSNHDEILPYNGSTPELEHIRKEISDVDDSDNTGTSNLVQEPIQIKDLEQ
                     EKQRLIKENRELSYAAAELRKQLEEVRIKNDKNLNKLREEIENAHQNYHELLTKINAV
                     GISEIDLKHVLPGKELEREENDLGAASSSLFTIRMYNTSEGVIGILHHPVDRAKLSIR
                     GVDGDAISSFISRHISRSIEEAHINEGKVMPDQVITEVESDSQEEENTPPEGDTLEIG
                     FRQNQTELPKGMPVLWKSAFKIKVTLKIPELSKRVGIPTNLSFCSLWLGLRKADSGGY
                     FLQESRALKLTPRSPDLHTELSFKGLEKGDYIVSCRATLPFLSRMLRGNAEFAVAEVL
                     TV"
     gene            complement(1479..2210)
                     /locus_tag="PKM23_08265"
     CDS             complement(1479..2210)
                     /locus_tag="PKM23_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91508.1"
                     /translation="MSIIQPLGAVTRTFGGEFDFPRGVIVFPDGSTLDPCAPGEAPNI
                     PPGDDDYTLMERWYDKAGFGKPKARQGWATMYEEGALISPEEEDPDVAPFVDLMLDRD
                     RGIVINHTARFSGRMVELINNRTFECRIRIAAHPLMAATGQVDLYDTDWVVIESFSSH
                     VPNKWFLLNTSTLVYTLRYLFPKSDISGDAPDSNYKLSVPGIYRLIIDWDVRHKMAGD
                     PDYHYEVLGGFDHNLVFRVGYQTSD"
     gene            complement(2407..3807)
                     /locus_tag="PKM23_08270"
     CDS             complement(2407..3807)
                     /locus_tag="PKM23_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNY91509.1"
                     /translation="MALRKEIILAVDEDPVFCRDLVRALPLSTSLKAVSDPSQALEAV
                     RSRCCSAILIAHTPPERDGIDLARKIREIMGAIPIVLVVPFTAVELIIDALHLGICDY
                     LEKPASTEAIAAMFSRLRVQIERSASNELSIPGTMNKQRKNILAGCTDLFAPGVQNSA
                     NGYLTVKMKRITSAIANICHVPSHRQHLQSMEKTPAMETLIPNDWPSVSVPSKVSKFS
                     FNFLGPFQCEIDGKRIEEWPGHKAKILFAYLAYHHKRHIYRDILMDYFWPHSSPDSAR
                     NCLNVTLHAIRATLHKIHPQLEGLLFKDECYYFCDEIDIELDTEKFLHFYDIAQHAER
                     RRENSDALGFYEAAAALYKGDFLEEYPYEVWTGLERENFAETYLVILDKMSDFYACDG
                     RPATAISLCDQILLKDNCREEIYRRLMVCYNRIGQRDRAIRVYKRCVEVLKTILDVEP
                     TTATQKLYQQIRGKVE"
     gene            4205..4630
                     /gene="arfB"
                     /locus_tag="PKM23_08275"
     CDS             4205..4630
                     /gene="arfB"
                     /locus_tag="PKM23_08275"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNY91510.1"
                     /translation="MPIEIDASTFIPDDEVGFTTSRSGGPGGQNVNKVETRVTLWFNV
                     EGSAVLSPGEKQLVTERLSSRINKEGLLWVTSSRHRTQYANRESAIDRFAALLREALA
                     PAPQRRPTRITPAARAERLKHKKRRSLVKQLRRERFSPE"
     gene            complement(4664..5293)
                     /locus_tag="PKM23_08280"
     CDS             complement(4664..5293)
                     /locus_tag="PKM23_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91511.1"
                     /translation="MNHSEESLIKAMGLEDRHGDGHQSGDGDELPLEAIAHRSIGKDQ
                     PDSDLEIPAVEKIEFKPAEAERTGVADEQSFPEEWSAPVAGEQKNVQYRITFNRQDLD
                     LQDLVLPLGVKQPFDLAIQLQLPLLHTGQAIPPIVSRLHVYLLCFDPSSGVRIRMERD
                     SQLIEPDKQQYIFPFHLEGFESGSWKLSVQMYAALFGFSERQVLNLKVG"
     gene            5533..7065
                     /locus_tag="PKM23_08285"
     CDS             5533..7065
                     /locus_tag="PKM23_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY91512.1"
                     /translation="MILLINPPAAKPCEPPAGLARLAGALEAHHLPVGVWDANREALL
                     ALLESAPAAGDHWTRQAWARSTTDIELLRSAAGYRHFSAYSAAVRRLNRLLAAHSAPA
                     EVVLSLADYQHRTWSPLRSGDLLAAAEAPERSPFYDYFRRALAARLEAAAPELIGLSL
                     SYLSQALPAFALIGLIRRDHPGLPIVIGGGLVTSWMRRPDWRNPFAGLIDHLIDGPGE
                     IPLLRLAGFHAPASGSFISYSAAEWPDYLAPGPILPYDASRGCYWNRCAYCPEPAEGH
                     GYQPRPVQQVLADLGRLTALHKPALIHFTDNALSPALLRALIEQPPGVPWYGFVRITT
                     QLMDQEFCCGLKKSGCAMLKIGLESGDDEVLEGMKKGVTVAQAGRALQALAAAGISTY
                     VYLLFGTPWEEEAAARRTLDFVVRHAGAIGFINPAIFNLPADAVDTLGLEVRSFYAGD
                     LQLYYDFNHPLGWERRQVRRFLDREFRREPSVAAILRRTPPSFTSNHAAFFVGRPQLT
                     SV"
     gene            complement(7123..7449)
                     /locus_tag="PKM23_08290"
     CDS             complement(7123..7449)
                     /locus_tag="PKM23_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91513.1"
                     /translation="MKKKIQEPLRLKYIQELAAELGALRTNFVDVISRYQSNQEARLL
                     ACIESLSAPAGHEAEDVHHDDRDILALLEELHGLRIKPDKGRLKDVRRMDELIDRVYK
                     RLVKLS"
     gene            complement(7480..8172)
                     /locus_tag="PKM23_08295"
     CDS             complement(7480..8172)
                     /locus_tag="PKM23_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HNY91514.1"
                     /translation="MKFFGEGLPGVNVDKLTGKLIAIEGADGSGRSTQIRLLTDWLEG
                     HGYATVNVGLKRSNLVSEELEAAMQGNVLSPITFSLFYATDFADQLENIIVPALRAGL
                     LVLADRYIYTLIARDIVRGGNPEWVESLYSIALVPDVVFYLDVAPRFLVERNMQKSAQ
                     LDYWESGMDIRLSRDIFDSFIRYQRLMRNEFRRMSEKYNFQVINGNRPPRSIALDLQK
                     HVAPLLGENRRS"
     gene            complement(8169..>8451)
                     /locus_tag="PKM23_08300"
     CDS             complement(8169..>8451)
                     /locus_tag="PKM23_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HNY91515.1"
                     /translation="VPPEVAVNRILDGRPTLKYHEAGMDLGLAADPYESFRIFQGRIN
                     DAYISMVPEYGFTVIDATQPIDVQQQLVRRIVSERIDLPDFKWKVRSRV"
BASE COUNT         1850 a         2384 c         2193 g         2024 t
ORIGIN      
        1 aggccaacaa taaagacact gaattcttcc ggagtcatca gcgcaaaatc acaactatca
       61 cttgttggga tactttcagc gacatggcca tcactggaac gggcttgtac aatttatacg
      121 gtcagcactt cggcaacagc aaactcggca ttccccctca gcatccggga caaaaatggc
      181 aaggttgccc tgcagctcac aatataatca cctttttcca aacccttgaa cgagagttct
      241 gtatgaaggt caggcgacct tggcgtaagc tttaatgctc tggattcctg cagaaaatac
      301 cctcccgaat cggctttgcg caatccgagc caaagactgc agaaggagag attcgtcggg
      361 atgccgacac gcttagataa ttcaggtatt ttcagagtta ccttgatttt aaaagcactt
      421 ttccataaaa ctggcatgcc tttaggcagc tcggtttgat tttgcctgaa tccgatttcc
      481 agagtatccc cctcaggcgg cgtgttctct tcttcctggc tatcactttc aacctctgtt
      541 atgacctgat cgggcatcac cttcccttca tttatatgtg cctcctcgat tgatctgctt
      601 atatgccgag atataaatga tgaaattgcg tcgccatcga cccctcgaat gctcagcttc
      661 gcacggtcta caggatgatg cagtattccg atgactccct ctgaagtatt gtacattctg
      721 atcgtaaaaa gcgaactgga tgctgcaccc agatcatttt cttctctctc cagttctttc
      781 cccggcagaa cgtgttttaa atcgatctcg gaaatcccaa cagcattaat ttttgtgagt
      841 agctcgtgat agttctgatg ggcattttca atctcttctc tgagtttatt caggttttta
      901 tcatttttta tccgtacttc ttccaactgc tttctcagct cggcagcggc ataacttagc
      961 tcccggttct ctttgatcag tctctgtttt tcctgttcga gatccttgat ttgaattggt
     1021 tcctgaacga ggtttgatgt tccagtgttg tctgaatcgt ccacatctga aatctctttt
     1081 ctgatatgct caagttcagg ggttgatccg ttatagggaa gaatttcatc atggttgctt
     1141 gattttttcc cgttcttgcg gttcacatgc tcattccaca attcccgaac aggtctgtcc
     1201 cccaacaaac tgaccggatc tgcctttgcc agtcttcttt tctctaaaga cggcgaggac
     1261 ttttgacggt tcgtttttgc tgatcttccc tttttcataa tcgaccctca aagaatggca
     1321 catacccagc catgtctgat ctcttcaaac aggaagcttg gcttaggtac gggcaaaatg
     1381 gaagtgtaac cgctttcctg gcctgaagtt aatcagaagg ccatcccgga ccctgggatt
     1441 gacaaactcc cagggtccca atggactttg atgtgttatc aatcactggt ctggtagccg
     1501 actcgaaaaa ccagattgtg gtcaaatccc ccgagaacct cgtaatgata gtcggggtca
     1561 cctgccatct tgtggcgaac atcccagtcg atgatcaatc ggtagatgcc aggcacggag
     1621 agtttatagt tggagtctgg cgcgtctccg ctgatgtcac tctttggaaa aagatagcgc
     1681 agggtataaa ccagagtgct ggtgttgagc agaaaccatt tgttggggac gtggctggag
     1741 aaactttcga tcaccaccca atcggtgtca tacaggtcca cctggcctgt agcagccatc
     1801 aacgggtgcg cggcgatgcg gatacgacat tcaaaggtcc tgttgttgat cagctcaacc
     1861 atccgcccgc tgaatcttgc agtatggttg atgacaatac cccggtcgcg atccagcatc
     1921 aggtccacaa aaggcgcaac atccggatct tcttcctctg gactgattaa ggccccctcc
     1981 tcatacatgg tcgcccatcc ttgccgagct tttggcttgc cgaatccggc cttgtcatac
     2041 cagcgttcca tcagcgtgta atcatcgtcg cccggaggaa tattcggagc ttctccggga
     2101 gcgcatggat cgagagtgga accatcggga aatacgataa ctcctctcgg aaaatcgaat
     2161 tcacctccga aggtccttgt taccgcacct aaaggctgaa tgatactcat ggtgtgactc
     2221 ctcccaaaag atgagtggtg cggcagctga gaaacgttca ccctggaacc ccggcctggg
     2281 tccgtttcag ttgcgcttca ctcaaatgat ggtgctggcc ggtgatttta taaactctcg
     2341 tgaacctgct ataagataat catcctgaat tagccctcga ttagagcact cttattttct
     2401 gcttaattat tcgacttttc cgcgaatttg ttgataaagt ttctgtgtcg ccgtagtagg
     2461 ttccacatcg agtatggttt ttaaaacctc cacacatcgt ttatataccc gtatcgctcg
     2521 atctcgctgg ccgatacggt tatagcaaac catcagccgc cggtatattt cttcccggca
     2581 attatctttc aacaagatct gatcacaaag agaaatcgct gtagccggcc ggccgtcgca
     2641 ggcataaaag tcactcattt tatccagtat caccagatag gtttcagcaa aattttcccg
     2701 ttcgagtcct gtccagacct catagggata ttcctcaagg aaatcccctt tataaagagc
     2761 tgccgccgcc tcgtaaaaac ccaaagcgtc cgagttttct cgtcttcttt cagcatgttg
     2821 cgcgatgtca tagaagtgaa ggaatttttc cgtgtccagc tcgatgtcaa tttcatcaca
     2881 aaaataatag cactcatcct tgaaaagcag accttccagt tgaggatgta tcttatgcaa
     2941 tgttgctcgg atggcgtgaa gagtcacatt caggcagtta cgggccgaat ccggggaaga
     3001 atgaggccaa aaataatcca tgagaatatc acgatagata tgacgtttat ggtggtaggc
     3061 caaatatgcg aagagaatct tggccttgtg ccccggccat tcttcaatac gtttgccatc
     3121 gatttcgcat tgaaaaggac caaggaaatt aaaactgaat ttgctgactt tgctggggac
     3181 ggaaactgat ggccaatcat tcggaatgag agtctccatg gctggtgtct tttccatgct
     3241 ctgcaggtgt tgtcgatgcg agggcacatg acatatgttc gcaattgcag atgttatcct
     3301 tttcattttt acggtcaagt aaccatttgc actgttttga acaccagggg caaatagatc
     3361 tgtgcagccg gcaaggatat tttttcgctg cttattcatc gttccaggga tggaaagctc
     3421 atttgaggca gatctttcaa tctggaccct gaggcgtgaa aacatggctg caatcgcctc
     3481 agtagaagct ggcttttcca ggtaatcaca aataccaagg tggagcgcat cgataatcaa
     3541 ctctactgca gtaaaaggaa cgacaaggac gattggaata gcacccatga tttcacggat
     3601 tttacgtgcc aagtcaattc catccctctc cggcggagta tgggcaatca ggatggcgct
     3661 gcagcacctg gagcggactg cttcgagcgc ctgggatgga tcggataccg ctttgagtga
     3721 agtcgataat ggcaatgcac gcacaagatc ccggcagaaa acgggatcct cgtcaacagc
     3781 caggatgatc tccttcctca gtgccatacc tccccctccg ggaaaactct ggtgagtaaa
     3841 taccccggct gttaaggccg ggatcttcac tgcccatcgc acggcaagat atcgactcgg
     3901 cgcaaagctg accctgcaat cggacagacc atgtccggtc acggaaaaca ctgcttgaaa
     3961 aggggtacaa gtacgtctgg aagagaatcg gaggagataa tatcgagagg agtcaccctg
     4021 cagccacccc tgagccccac tgctgatgcc taattaaaaa tacatcggga gcaaggggaa
     4081 agcaagcaat attttgtttt taagtgtttt ttaagcgcag accgcatatg aatgcggaat
     4141 ggtttggttt tgtccccatt atttgataca tttttccctg aaatcatcaa acggaaccat
     4201 tgccatgccc atcgaaatcg atgcatccac attcatccct gacgacgagg ttggtttcac
     4261 cacctcgcgc agcggcggcc ctgggggaca aaacgtcaac aaggtcgaga cccgggtaac
     4321 cctctggttc aatgtggagg gcagcgcagt cctctcgccg ggagagaagc agctcgttac
     4381 tgaaaggctt tcctcgagga tcaacaagga ggggctgctt tgggtcactt ccagccgcca
     4441 tcgcacccag tatgccaacc gcgagagcgc gatcgatcgg ttcgccgcgc tcctgcgcga
     4501 ggcgctggct ccggcccccc agcgcaggcc gacacggatc actcccgccg ccagagctga
     4561 acggctcaag cataaaaaaa ggcgcagcct ggttaaacag ctgcgccgtg aaagattcag
     4621 tccggagtga tcagccggaa ccgcggaaag cccgcctttt ctgctatccc accttgagat
     4681 taagcacctg gcgttcacta aaaccaaaaa gggcggcgta catctgtaca ctcagcttcc
     4741 atgaacccga ctcaaaaccc tccagatgga aagggaagat gtactgctgc ttgtccggtt
     4801 cgatgagctg agagtcccgt tccatgcgga tgcgcacccc gctgctggga tcgaagcaga
     4861 gcagatagac atgcagcctg gatacgatcg ggggaatggc ctggcccgta tggagcagcg
     4921 ggagttggag ctggattgca aggtcaaagg gctgcttgac ccccagaggc aggacgagat
     4981 cttgcaaatc aagatcctga cgattgaaag tgatccggta ctggacgttt ttctgttcgc
     5041 cggcaacagg cgcggaccac tcctccggga aggattgctc gtcagcgacc ccggtgcgct
     5101 cagcctccgc cggcttgaat tctatctttt ccaccgcggg aatctccaga tcgctgtcgg
     5161 gttgatcctt gccgatgctg cgatgggcga tggcttcaag cgggagttcg tcaccgtcgc
     5221 cggactggtg tccatcgcca tggcgatctt ccaggcccat ggccttgatc agggattctt
     5281 cactgtgatt caataaggcg gagagattac ggccgaggcg agattttggt tttgcgttgg
     5341 aatcgttcat gtgtacccct cctcccccca cagatgatac caaattacgc taaattacgt
     5401 catccagttc taaattacaa gcatattttt catttttccg ccgcggctgg aggggtgaag
     5461 atatgcttat tggttgcgaa tggctctgca attcgataga ttatccctac aagagcttca
     5521 atcgagaaag ccatgattct gctgatcaat cccccagccg ccaagccctg cgaacccccg
     5581 gccgggctgg cgcgccttgc cggtgcgctg gaagcccacc acctgccggt cggagtctgg
     5641 gacgccaatc gcgaagcact gctcgccctg ctggagagtg cacccgcggc cggcgaccac
     5701 tggacgcgtc aggcctgggc gcgcagcacc actgatatcg aactgctcag aagcgccgca
     5761 ggctatcgcc atttcagcgc ctacagcgcc gccgtgcgcc gcctcaaccg gctgctcgca
     5821 gcccacagcg ctcccgccga ggttgtcctc tcgctggccg actatcagca ccgcacctgg
     5881 tcgcccctgc gcagcgggga tctgctcgct gccgccgagg cgcctgagcg cagccctttt
     5941 tatgactatt tccgcagggc tcttgcggcc cgcctggagg cggccgcacc tgagctgatt
     6001 ggcctatcgc tctcttacct cagccaggcc ttgcctgcct ttgccctgat cggcctgatt
     6061 cgccgtgacc atcccggcct gccgatcgtc atcggcgggg ggctggtcac gtcgtggatg
     6121 cgccggccgg attggcgcaa tcccttcgcc gggcttattg atcacctcat cgacggccct
     6181 ggtgaaatcc cgctgctcag gctcgccgga tttcacgcgc cggcctccgg ctcgttcatc
     6241 tcctattccg ccgcggaatg gcccgattac ctcgctcccg gcccgattct cccctacgat
     6301 gcctcgcgcg gctgctactg gaaccgctgc gcctactgcc cggagccggc ggaggggcac
     6361 gggtatcagc ctcgcccggt gcagcaggtg ctcgccgatc tgggccggct gacggccctg
     6421 cacaagccgg ccctgatcca tttcaccgac aacgccctca gtccggccct gctgcgggcc
     6481 ctcatcgaac aaccgcccgg cgtcccatgg tacggctttg tccgcattac aacacagctg
     6541 atggaccagg agttttgctg cggcctgaaa aaatcgggct gtgccatgct caagatcggt
     6601 ctggaatcgg gggatgacga ggttctcgag gggatgaaaa agggggtaac agtagcgcaa
     6661 gccggacggg cgctgcaagc cctggccgca gcggggattt ccacctacgt ctatcttctc
     6721 tttggtacgc cctgggagga ggaggccgca gcccggcgca cactcgactt tgtcgtccgt
     6781 cacgccgggg cgatcggctt tatcaatcct gcaatcttta accttccggc tgatgcggtc
     6841 gataccctcg gactcgaggt gcgcagtttt tacgcgggcg acctgcagct ttattatgat
     6901 ttcaaccatc ccctgggttg ggaacggagg caggtgcgcc gcttcctcga tcgcgaattc
     6961 aggcgggagc cctccgtcgc cgccatcctg cggcgcacac ccccctcctt cacctccaac
     7021 cacgccgcct tcttcgtcgg ccgcccgcaa ctcaccagcg tctgagcagc ccttaacatg
     7081 acgaggatgt ggagaagcgt ctgagaggaa aaagcccgga tatcaggaga gcttgaccag
     7141 ccgtttgtag acccggtcaa tcagttcatc catccggcgc acatccttga gccggccttt
     7201 gtccggcttg attcgcagtc cgtgcagctc ttcgagtaaa gcgagaatgt ctctgtcgtc
     7261 gtggtgaaca tcctccgcct catgcccggc cggcgctgac aggctttcga tgcaggccag
     7321 aagccttgcc tcctgattcg actggtaccg gctgatgaca tcgacgaaat tggtgcgcag
     7381 cgcgcccagt tcggcggcca gctcctgaat gtattttaac cgcaggggtt cctggatttt
     7441 tttcttcata gccatccatt tgcatgcatg atgccctgat tagctgcgcc tgttctcccc
     7501 caacaggggt gcgacatgct tttgcaggtc gagggcgatt gagcgcggcg gccggttgcc
     7561 gttgatgact tgaaagttgt atttctcgct catccggcgg aattcattgc gcatcagacg
     7621 ctgataacgg ataaagctgt cgaaaatatc tcgcgacaga cggatatcca tccccgactc
     7681 ccagtagtcg agctgggcgc ttttttgcat gttgcgctcc accaggaagc gtggagccac
     7741 gtcgaggtaa aagaccacat ccggaaccag agcgatgctg tagagcgact cgacccattc
     7801 gggattgccg ccgcggacga tatcgcgcgc gatcagggta tagatgtagc ggtccgcgag
     7861 gacgagcagg cccgccctca gtgccgggac gatgatgttt tccagctgat cagcgaaatc
     7921 agtggcgtaa aacagagaga aggtgatcgg gctaagcaca ttgccctgca tcgcggcctc
     7981 cagctcctcg ctgaccagat tcgagcgctt caggccgacg ttgaccgtgg catagccgtg
     8041 cccctcaagc cagtcggtga gcagcctgat ttgcgtggaa cggccgctgc cgtcggcccc
     8101 ctcgatggca atcaattttc cggtgagctt gtccacgttg actccgggca gtccctcgcc
     8161 aaagaacttc ataccctgct cctcactttc catttaaagt ccggcagatc gatgcgctcg
     8221 ctgacaatcc tgcggacgag ctgctgctgc acatcgattg gctgggtggc gtcgatgacg
     8281 gtaaagccgt attcggggac catgctgata taggcgtcgt tgatcctgcc ctgaaagatg
     8341 cggaagctct cgtagggatc ggcggccagc ccgagatcca tgccggcctc gtggtacttg
     8401 agggtgggcc tgccgtcgag gatgcggttg acggccacct ccggggggac c
//
LOCUS       DAOPWU010000135         8445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8405_length_8445_cov_5.009774, whole genome shotgun sequence.
ACCESSION   DAOPWU010000135 DAOPWU010000000
VERSION     DAOPWU010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8405_length_8445_cov_5.009774"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="PKM23_08305"
     CDS             <1..1630
                     /locus_tag="PKM23_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91516.1"
                     /translation="YEDPYNSVSYIADTTFAEDGTAQISYTIGRDSEGYVHGDSLTAP
                     SNWSFADAGTQLQHFKRNTTYDVAKFDITSQITKNHQLKAGLEGRFNKLTLDEFYIIP
                     AEEGNEQIVPFLPSIPDRRTPDHNGYSQKPVEFSLYAQDKMEFNDMIVNFGLRFDYFN
                     SRGKVLADPKDPNIYDPFDPYNKYKNYTPDTPAEELVEYTVAERQAFWYKDATAKMAL
                     SPRFGIAYPITDRGVIHFSYGHFFQIPTFRQLYGANDGTDTSNPDLEVTTTSGNSTFL
                     SNADLKPQRTVQYEIGLSQQITGDLGAELTMFYRDVRDWVGASYSIPTYLASVAYTQF
                     ENKDYSNVKGVTLALTKRYSSYFEAGLDYSFMVAEGSASDPKDSYNDRLSDKAPRIQL
                     TPLGWDQRHTINGNLSVGTTTWRASLLVKYWTGTPYTPTFPPGVSSGSSSFSGLADNS
                     ARKPAINSTDLRLFKTFRIGKQNLSLFAYIYNLFDQRAATNVWSDSGSPKYTLQTRNA
                     SFDANRVSTVEDNANHPEWYIEPRQVQLGFSLGF"
     gene            1689..5339
                     /locus_tag="PKM23_08310"
     CDS             1689..5339
                     /locus_tag="PKM23_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91517.1"
                     /translation="MKKTIIMTLITGLVLLAAAAFSQPKPHGMFSEERKGFHNGNRIQ
                     TTFYNSGLIGRVSGIEEDIAGEWPKGSGHLYIGDQLMMVGAEIIDRDKKLIHSVITPR
                     GPKVDPRMGDRSSDGLTWYTWEALPGFAGKDTSVVAMSHIPKSWPVYWPDKQEITDDP
                     GWRNDGYDNDSKRAAWNGYFGKNQFNADQESYFVMDDYNDAKYNFYPDSTDLSRRGLG
                     LQASSRGLQWAQVLAQDVLFFIYDITNIGTTTYDKVVFSIICGYMAGGDGEDDNCTFD
                     RSINLTYTWDYDGIGSGGWSPVDIAGCAFLESPGNFRDGIDNDGDGAMGSGKTITEDM
                     FKPAQLAAGQDIVVISYGNNFERSVQKMPADSLIITGPKGNRIVFRPGQQVEEIRFNG
                     VDDNLNGLIDENNFNEIEITTGVTQKNYVYVGLKYVNYITGEGMDNKLIDERRDDGID
                     NDGDWNPITDDVGLDGQFNTMDFGEGDGLPTSGAGTSAPGEPHVDKTDVSESDQLGLT
                     SFYFFYPFNKFGLKEDEKLWTYSTPGSFVNGVGNNVDGDWIYASGYFPLKPGETERIS
                     TAIIYSAWVPGDPRSGIVAKKATAQFIYDENYNFAKAPNPPKVWASVGDGEVTIYWDD
                     SAEFSYDAVSGYDFEGYRIYRASDPGFEDASPVTDYLGSRLMSVPLAQYDRKDGISGF
                     YPKSYPGTGVEFYLGNDSGLAHSFRDTTVINGYTYYYAVTAYDRGDLVNGFNPVECPI
                     DISIDAAGNVSLGSNVVSVVAAARAAGYQPRENMIALEKQAGSVGTGSVQAEIVDEKM
                     IPDRHDLLVRFSDTSSDGIDNDGDWDKLHHDLGADGIAGTGDAGEGDGLPTVGEPNLD
                     NNDLQEWVPLTTGYSVFDVTNPANPVTLVETTFIQLAEGQAIPDTVVDTSGDIDGGRD
                     FFYGMRLNIENASRVEKIIEKSNWNIIRTTAPMNYAYVFNKFSFAGYYEVGVEYPVNY
                     AVVALDDFSGQSSPLTIHRKNANGTMGAAYPIPGVKTNFKVVNMDTRTEIPYAFLDYA
                     VRPSFIEPGQLSNFDRIIFYETVGAETKVTWSLYFAGNDTTTYHPKAGDTLRVVTTRP
                     FGSQDSFRFTTRAAEVDAAQAAAELKNIKVVPNPYVASAGWEPVNPYATGRGSRELHF
                     THLPMKCTIRIYTVQGELIATLDHNASFYNSQAKWNMLTKDNLDIAYGIYVFHVDAGE
                     LGSHVGKFAVIK"
     gene            5408..6427
                     /locus_tag="PKM23_08315"
     CDS             5408..6427
                     /locus_tag="PKM23_08315"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY91518.1"
                     /translation="MKKMIITGLIGLALVTAGYAQNVTKVGTTAAPFLNVGMGARAIA
                     MGGAYVSMANDATALFWNPAGIATMQGSEAVFNHSEWIADINFDFAGVALNLGNIGSF
                     GLFGNFMTIGEMDVTTELYPDGTGQRFNSGSYTMGISYARSLTDRFAIGANVKYVHEY
                     IMNSGANGIALDIGTVFTTQFRGLRLGAAITNFGTKMRMTGRDLLVQHDTDPLREGNN
                     DKINANMATDAYEMPLYLRVGMSYNVLQDTKKHALWVAVDAVHPSDNVEMVNLGAEYA
                     FDNMFFLRGGYASLFSEDAEQGLTLGAGLRYGFAGRMALKVDYAYEAFGRFDYIQKFT
                     LGIGF"
     gene            6564..8060
                     /locus_tag="PKM23_08320"
     CDS             6564..8060
                     /locus_tag="PKM23_08320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNY91519.1"
                     /translation="MKKVLILITLLLACDFRLLDDQTAALVNKEPISLEEVRNNIHFM
                     TLYARNLKGRELVDAHLDLLIEKKLFAQEGKRLGFAQTPLVRQVTDWAERDQMIKALY
                     RDEVYNKAVPTEEEIRAAFFRGREQVHVRHLFARSEVEALHLKEELDRGASFEALAAR
                     TFRDSTLARSGGDLGFVGYEDIDEKLAETAFALPKGMISPPVRSRYGWHILRVEGKRQ
                     QIFNSEAEYSQKREAIAADLTRAMQRKMAGAYVDRMMSALDVRMLNTAFNTLASQVKE
                     TVLNADRVVPNYQPMLGGTELLMVNDGVSGHGGQVLVIWKGGQMTLGDFMKRVEALPV
                     SERPRIDTPGHLRFDIGKLVMRELLLQEARRRNLDKDPMVQEGVRKWREEYLFATLWQ
                     RVRDTITVNSAEEEAFFAVHQGRYREFLQHRPQGLLPSDSLSAEPRLQNQVREDLLGE
                     KTDQLYKKTAAELLGRARIRRNDKMLEALAREFPRSGERIDMMGLPNK"
     gene            8080..8238
                     /locus_tag="PKM23_08325"
     CDS             8080..8238
                     /locus_tag="PKM23_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNY91520.1"
                     /translation="MSKLSILREFWLFMRERKKWWLGPIIFFLVLLGLLIVFAEGSAL
                     APFIYTLF"
     gene            8249..>8445
                     /locus_tag="PKM23_08330"
     CDS             8249..>8445
                     /locus_tag="PKM23_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91521.1"
                     /translation="MTRHDRNKRHSAASAAAPSRLRAGLFWALLLLIPILFFLLLEGG
                     LRLFDYGGNDALVLLREDYGER"
BASE COUNT         1765 a         2819 c         2261 g         1600 t
ORIGIN      
        1 ctacgaagat ccctacaact cggtctccta tatcgccgac accaccttcg ccgaggatgg
       61 caccgcccag atctcctaca ccatcggcag ggattccgag ggctatgtcc atggcgattc
      121 cctgacggcg ccctccaact ggagctttgc tgacgccggc acccagctgc agcatttcaa
      181 gcgcaacacc acctacgatg tcgccaagtt cgacatcacc agccagatca ccaagaacca
      241 tcagctcaag gccggcctcg agggccgctt caacaagctc accctcgatg agttctacat
      301 cattccggcc gaggagggca acgagcagat agtgcccttc ctcccctcca tccccgaccg
      361 cagaaccccg gatcacaacg gctactccca gaagccggtc gagttttcgc tttatgccca
      421 ggacaagatg gaattcaacg atatgatcgt caactttggc ctgcgcttcg actatttcaa
      481 ttcccggggc aaggtgctgg ccgatcccaa ggatcccaat atttacgatc cctttgatcc
      541 ctataacaag tacaagaact atacccccga cacccccgcc gaggagctgg tcgagtacac
      601 cgtggctgaa cgccaggcct tctggtacaa ggatgccacg gctaaaatgg ccctcagtcc
      661 gcgcttcggc atcgcctatc cgatcaccga ccgcggcgtc atccacttct cgtatggtca
      721 tttcttccag atcccgactt ttcgccagct ctacggcgcc aatgacggca ccgacacctc
      781 caacccggat ctcgaagtga cgaccacctc gggcaactcg acctttctca gcaatgcgga
      841 cctcaagccc cagcgcacgg tgcagtacga gatcggcctc tcccagcaga tcacggggga
      901 tctgggcgct gagctgacca tgttctatcg cgacgtccgc gactgggtgg gcgcgagcta
      961 ctccatcccg acctacctgg ccagcgtcgc ctatacccag tttgagaaca aggattactc
     1021 caacgtcaag ggcgtgaccc tggccctgac caagcgctac tccagctatt tcgaggccgg
     1081 cctcgattac agcttcatgg ttgccgaggg gagcgcctcc gatcccaagg actcgtacaa
     1141 cgaccgcctc agcgacaagg ccccgcgcat ccagctcacc ccgctgggct gggaccagcg
     1201 ccacaccatc aatggcaacc tgagcgtcgg caccaccacc tggcgtgcca gcctgctggt
     1261 caagtattgg acgggcaccc cctacacccc gacctttcct cccggggttt cctccggcag
     1321 cagctcgttc agcggcctgg cggacaacag cgcccgcaaa cccgcgatca actccactga
     1381 tctgcgtctc ttcaagactt tcaggatcgg caagcagaat ctctcgctct tcgcctatat
     1441 ctataacctt ttcgatcagc gcgccgctac caacgtctgg agcgacagcg gcagtcccaa
     1501 atacaccctg cagacccgca acgccagttt tgacgcaaac cgcgtcagca ccgtcgagga
     1561 taatgccaat catcccgaat ggtacatcga gccccggcag gtgcagcttg gcttctcgct
     1621 gggtttttaa cccgcgttgt gcagggtgtt tcgagagcga accgagttaa cataagggac
     1681 catacagcat gaaaaaaaca atcataatga cgctcatcac gggacttgtt ctgctcgccg
     1741 ctgcggcttt cagtcagccc aagccccatg gcatgttcag cgaggaacgc aagggctttc
     1801 acaacggcaa ccgcatccag accacctttt acaattccgg tctgatcggc cgcgtctccg
     1861 gcatcgaaga ggacattgcc ggcgaatggc ccaagggttc cggccacctc tacatcggcg
     1921 accagctcat gatggtgggc gccgagatca tcgacaggga caaaaagctg atccactccg
     1981 tcatcacccc gcgcggcccc aaggtcgatc cccgcatggg cgaccgctcc tccgacggcc
     2041 tcacctggta cacatgggaa gccctgcccg gcttcgccgg caaggatacc tcggtggtgg
     2101 ccatgagcca cattcccaaa tcatggccgg tctactggcc cgacaagcag gagatcaccg
     2161 atgatccggg ctggcgcaac gacggctacg acaatgattc caaacgcgcc gcctggaacg
     2221 gctatttcgg caagaaccag ttcaacgccg accaggaaag ctactttgtc atggacgatt
     2281 acaacgacgc caagtataat ttctatcccg actctaccga tctttccagg cgcggcctcg
     2341 gcctgcaggc ctcatcgcgc gggctgcagt gggcccaggt gctcgcccag gatgtgctct
     2401 ttttcatcta tgacatcacc aatatcggca ccaccaccta tgacaaggtc gtcttcagca
     2461 tcatctgcgg ctatatggcc ggcggcgacg gcgaggatga caactgcacc ttcgatcgct
     2521 cgatcaacct gacctatacc tgggattatg acggcatcgg ctcgggcggc tggtcgccgg
     2581 tcgacatcgc cggctgcgcc ttccttgaaa gccccggcaa tttccgtgac ggcatcgaca
     2641 acgacggcga cggcgccatg ggttcgggta aaaccatcac cgaggatatg ttcaagcccg
     2701 cccagctggc cgccggccag gacatcgtag tgatctccta cggcaataat tttgaacgca
     2761 gcgtgcaaaa gatgcccgcc gactccctga tcatcactgg ccccaagggc aaccgtatcg
     2821 tcttccggcc gggacagcag gtcgaggaga tccgcttcaa cggcgtcgac gacaatctca
     2881 acggcctcat cgacgaaaac aactttaatg agatcgaaat caccaccggc gtcacccaaa
     2941 agaactacgt ctacgtcgga ctaaagtacg tcaactacat caccggcgag gggatggaca
     3001 acaagctcat cgacgaacgc cgcgacgacg gcatcgacaa tgacggcgac tggaatccga
     3061 tcaccgatga tgtcggcctc gacggtcagt tcaacaccat ggactttggg gagggcgacg
     3121 gcctgcccac ctcaggcgcc ggcaccagcg ccccgggcga accccacgtt gacaaaaccg
     3181 acgtcagcga atcggaccag ctcggcctga caagttttta cttcttctac cccttcaaca
     3241 agttcggcct caaggaagac gagaaactgt ggacctactc gacccccggc tccttcgtca
     3301 acggcgtcgg caacaacgtc gacggcgact ggatctacgc ctccggttac ttccccctca
     3361 agccgggtga gaccgagcgc atctccaccg ccatcatcta ttcggcctgg gtcccgggcg
     3421 atccgcgcag cggtatcgtc gccaaaaagg cgaccgccca gttcatctat gacgaaaact
     3481 acaatttcgc caaagccccc aaccccccca aggtttgggc ttcggtcggc gatggcgagg
     3541 tcaccatcta ctgggacgac tcggcggaat tctcctacga cgcggtcagc ggctacgatt
     3601 tcgaaggcta ccgcatctat cgcgcttccg atcccggctt cgaggatgcc agtccggtca
     3661 ccgactatct cggcagccga ttgatgtctg tgccgctggc ccagtatgac cgcaaggatg
     3721 gcatttccgg cttttatccc aagagctatc ccggcaccgg cgtcgagttc tacctcggca
     3781 acgattcggg tctggcccat tccttccgtg acaccacggt aatcaacggc tacacctact
     3841 attatgcggt cactgcctat gatcgcggtg atctggtcaa cggcttcaat ccggtcgaat
     3901 gcccgatcga tatcagcatc gatgccgccg gcaacgtctc cctcggctcc aatgtggtgt
     3961 cggtcgtggc cgctgcccgg gcggcgggct atcagccgcg cgaaaacatg atcgccctcg
     4021 aaaaacaggc gggttccgtc ggcaccggct cggtccaggc cgagatcgtg gatgaaaaaa
     4081 tgattcccga ccgccatgac ctccttgtcc gcttctccga cacctccagc gacgggatcg
     4141 acaacgacgg cgactgggac aaactccatc atgacctcgg cgccgacggc atcgccggca
     4201 ccggcgacgc cggcgaaggc gacggcctgc cgaccgttgg cgagcccaat ctggacaata
     4261 acgatctcca ggagtgggtg ccgctgacca cgggctacag cgtcttcgac gtcaccaatc
     4321 ccgccaatcc ggtcaccctg gtggagacca ccttcatcca gctcgccgag ggtcaggcga
     4381 ttcccgatac tgtggtcgac accagcggcg acatcgatgg cgggcgcgac tttttctacg
     4441 gcatgcgcct taacatcgag aacgccagca gggtcgagaa gatcatcgaa aaatcgaact
     4501 ggaacatcat ccgcaccacc gccccgatga actacgccta tgtttttaac aaattctctt
     4561 tcgcaggcta ttatgaggtg ggtgttgagt atccggtcaa ttatgccgtc gtcgccctgg
     4621 atgatttcag cggccaatcc tcgcccctga ccatccaccg caagaacgcc aacggcacca
     4681 tgggcgcggc ctatcccatc cccggggtca agaccaactt caaggtggtc aatatggaca
     4741 cccggacgga gattccctac gcttttctcg attatgctgt ccgcccctcc ttcatcgaac
     4801 cggggcagct ctccaatttc gaccgcatca tcttttacga gacggtcggc gcggagacca
     4861 aggtgacctg gagcctctat ttcgccggca acgacaccac cacctatcac cccaaggcgg
     4921 gtgataccct gcgcgtcgtg accacccggc ccttcggcag ccaggactcc ttccgcttca
     4981 ccacccgtgc ggctgaggtc gatgccgccc aggctgcggc ggagctgaag aacatcaagg
     5041 tggtgcccaa tccctatgtc gcctccgcgg gctgggagcc ggtcaatccc tacgccaccg
     5101 gccgcggctc gcgcgaactc cacttcaccc atctgccgat gaaatgcacc atccgtatct
     5161 acaccgtgca gggagagctg atcgccaccc tggaccataa cgcctccttc tacaacagcc
     5221 aggccaaatg gaacatgctc accaaggaca atctcgatat cgcctacggc atctatgtct
     5281 tccacgtcga cgccggtgag ctgggcagcc atgtgggcaa attcgcagtg atcaagtaga
     5341 acaatgagag cttccgggag gtcctcctcc cggaagcgtc atggaacgca aatatgcaga
     5401 ggtttctatg aaaaaaatga tcatcaccgg tctcattggg ctcgccctgg tcactgcggg
     5461 ttatgcacaa aatgtcacca aggtgggcac cacggccgcg ccgtttctca acgtcggcat
     5521 gggggcacgc gccattgcca tgggcggggc ctatgtctcc atggccaatg acgccacggc
     5581 tctcttctgg aacccggcgg gaatcgccac catgcagggg agcgaggcgg tctttaacca
     5641 ctccgaatgg atcgccgata tcaactttga cttcgccggc gtcgccctca accttggcaa
     5701 catcggctcc ttcggcctct tcggcaattt catgaccatc ggcgagatgg atgtcaccac
     5761 cgagctctat ccggacggga ccgggcagcg cttcaattcc ggttcttaca ccatgggaat
     5821 cagctatgcc cgttcgctga ccgaccgttt cgccatcggc gccaacgtca agtatgtcca
     5881 tgaatacatc atgaacagcg gggccaacgg catcgccctc gatattggga cggtcttcac
     5941 cacccagttc cgcggcctcc gtctcggcgc cgccatcacc aacttcggca ccaagatgcg
     6001 catgaccggc cgggatctgc tcgttcagca tgacaccgat cccctccgtg agggtaacaa
     6061 cgacaagatc aatgccaata tggccaccga cgcctacgag atgcccctct atctgcgcgt
     6121 gggcatgtcc tacaatgtgc tgcaggatac gaaaaaacac gccctctggg tggccgtcga
     6181 tgccgtccat ccgagcgaca acgtcgagat ggtcaacctc ggcgccgaat acgctttcga
     6241 caacatgttt ttcctgcgcg gcggctatgc ctccctcttc tccgaggacg ccgaacaggg
     6301 gctgaccctg ggggcggggc tgcgctacgg ctttgccggc aggatggccc tcaaggtcga
     6361 ttacgcctat gaggcctttg gccgcttcga ttatatccag aaattcaccc tcggcatcgg
     6421 tttctgaccc tgctcccgac agtccggccg ccttcaccgg ggccgggctt cgccggggtt
     6481 gtctccgcac catggggaga gagccagccg ctttctcccc ttttgcctta tcgccggacc
     6541 ccttgccgta acctgggccg cctatgaaaa aagtcctcat cctcattacc ctgctgctcg
     6601 cttgtgattt caggctcctg gacgaccaga ccgccgcttt ggtcaacaag gagcccatct
     6661 ccctggagga ggtgcgcaac aacatccatt tcatgaccct ctatgcgcgc aatctcaagg
     6721 ggcgcgagct ggtcgatgcc cacctcgatc tcctcatcga gaaaaagctc ttcgcccagg
     6781 agggcaaaag actgggtttt gcgcagaccc cactcgtccg ccaggtcacc gactgggcgg
     6841 agcgcgacca gatgatcaag gccctctacc gcgacgaggt ctacaacaag gccgtgccga
     6901 cggaggagga gatccgcgcg gcctttttcc gcggccgcga gcaggtgcac gtgcgccacc
     6961 tcttcgcccg ctccgaggtg gaagccctgc atctcaagga ggaactcgac cgcggcgcct
     7021 ctttcgaggc cctggccgcc cgcaccttcc gcgactccac cctcgcccgc agcggggggg
     7081 acctcggttt tgtcggttat gaggatatcg atgaaaaact ggccgaaacc gctttcgccc
     7141 tgccaaaggg gatgatctcg ccaccggtgc gcagccgcta cggctggcac atcctgcgcg
     7201 ttgaaggcaa acgccagcag attttcaaca gcgaggcgga gtacagccag aaacgcgaag
     7261 ccatcgccgc tgacctcacc cgtgccatgc agcgcaagat ggccggcgcc tatgtcgacc
     7321 gcatgatgag cgccctcgat gtccgtatgc tcaatactgc ctttaataca ctcgcctctc
     7381 aggtcaagga gaccgtcctc aacgccgatc gggtggtgcc caactaccag cccatgctgg
     7441 gcggcaccga gctgctcatg gtcaatgatg gcgtcagcgg ccacggcggg caggtgctgg
     7501 tgatctggaa gggagggcaa atgaccctcg gggactttat gaagagggtg gaggcgcttc
     7561 cggtcagcga acgcccgcgg atagacaccc cgggccatct gcgcttcgat atcggcaagc
     7621 tggtgatgcg cgagctgctc ctgcaggagg ccaggcgccg gaatctcgac aaggatccca
     7681 tggtgcagga gggagtgcgc aaatggcgcg aagagtacct tttcgcgacc ctctggcagc
     7741 gggtgcggga caccatcacg gtcaacagcg ccgaggagga ggcctttttc gctgtgcacc
     7801 agggccgcta tcgcgagttt ctccagcacc ggcctcaggg cctgctcccg tccgactcac
     7861 tctccgctga gccccgcctg caaaaccagg tgcgcgagga tctcctcggc gaaaagaccg
     7921 accagcttta caaaaaaacg gctgcggaac tgctcggcag ggccagaatc cgccgcaatg
     7981 acaagatgct cgaggccctc gcgcgcgagt tcccccgcag cggtgaacgc atcgacatga
     8041 tggggctgcc caacaaataa tcagaggtcg aggttatcca tgtccaaact ttccatcctg
     8101 cgtgaattct ggctcttcat gcgcgaacgc aaaaaatggt ggctgggtcc catcatcttt
     8161 tttctggtcc tgctcggact gctgatcgtc ttcgccgagg gatccgccct ggcgcctttt
     8221 atctatacct tgttctgaca ggcgatccgt gaccaggcat gaccggaaca aacgccacag
     8281 cgccgcatcg gctgcggccc catcccgcct gcgcgcagga ctcttctggg ccctgctgct
     8341 cctgatcccc atcctctttt ttcttctgct cgaggggggg ctgcggctct tcgactacgg
     8401 cggaaatgac gctctggtgc ttttgcgaga ggattacggc gaacg
//
LOCUS       DAOPWU010000136         8420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8434_length_8420_cov_4.170831, whole genome shotgun sequence.
ACCESSION   DAOPWU010000136 DAOPWU010000000
VERSION     DAOPWU010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8434_length_8420_cov_4.170831"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="PKM23_08335"
     CDS             complement(<1..1650)
                     /locus_tag="PKM23_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91522.1"
                     /translation="MKSTPVACFLLLLVIIPALLMGQGRKYEGPDDPAGDIAAIREGW
                     MNGNRVLLYFKNTTELANWPNFDQSKWPNDYTGTGMHDGIALWIVARVHMADGSTPVT
                     DPAEIASRSDLQELYFCQTSYRMGMDHSKDGLIRWGLYPVFGYFNESSEYPAMSNRED
                     SWPAAGWPSAGSTLKWPGEWDGRFGRGVKYADLETYFVANDAQDLEYVQPDAPLKYYP
                     RPGVKIGDNDPDVTIQKGEPWGGLGIRVKVRGYQWNNPQTRDAIFWEYDISNISDHDL
                     ADVAFGYLLDAGIGHRGDQGETDDIGFFDKYEDISYSWDYDGIGIGGIRTGIMGMAFL
                     ESPGRWNDGIDSDDDGLVDEKRDNVATRLIGPTEGISDMAKFLTWYGLAEEQLKTHWD
                     ADEDQDWQDGTDANNNGVYDIGEDFGNDIGTDGVAPTDLNYTGPDADGSECNHKPDFV
                     EGLGCEPNFGLTDISESDMIGLTGFQMLHHPQENEPPFSAFDKEAYEQIASPRLEEYF
                     GELANLVMTFGSGTFRLPRGLTERISMAELHSYEELSGLNSP"
     gene            complement(1694..4414)
                     /locus_tag="PKM23_08340"
     CDS             complement(1694..4414)
                     /locus_tag="PKM23_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91523.1"
                     /translation="MAKYLTAALLCLILTAAPLLGQTTGKIAGRVSDLTSGETLTGAN
                     ILLEGTSRGATADINGEFFIINLEPGTYTVRVSMVGYETLRMQNVRVSVNRTFELTAK
                     LKSTVISGQEVTVVADRVAVKKDQTSSVRNVSSTQMDILPTESVSAVVAMQPGVVLGH
                     FRGGRSDEVTYLIDGMQVDESYGRGGRTVNVEKDVVEDLEVITGIFSAKYGNAMSGVV
                     NAVTKDGGRKFHGSASLGLGNYLTPHKDIFIGLQDTDFTRRQDYQFALNGPIIGDRFT
                     FATNVRYQNNKGHLNGIRRFLPDDYSDFTSSDSSQWYTEHSGDNAYVAMNTAKNLNVW
                     GKLTMRPSSRIKTSLQYILNDDEGQGYSHSMKYNPDGRSTGYHTSHLASLQWNHMLSD
                     KAFYELKGSYVSDNNASYMFKDPYDSRYIHDLYSRSEGPGFSTGGQSKGHSERTLEDY
                     TGKGDFVWQLHPQHSIEGGAQYSQKVLENEWFTIRNKYYGTAFETLWDVDPETGKRRY
                     LFYEPMVMPDSTLYHEYYKVKPVEFSAYLQDKMEFAEMVINLGLRLDYFDPKITYPSQ
                     WRNPSNQLSFPGDPERMSHPVEADPKYQISPRLGISYQLGKTALLRFGYGHFLQMPPL
                     YAMYDNYARLVPPGDYSTTMGNPQVNAQKTIQYEVGLWQELMPDMSLEVALFYRDIYD
                     LLSARIITTYNQIRYGLYSNKDYANARGLELKYEYIAGRLSVVANYTLQFSRGNADTP
                     TSTFSRAGSQRDPVNKLIPMSWDQRHTANVTVGYSMPRYGATMTVYYNSGSPYTWSPL
                     ADSPLSRVNLFPNNEHIPSSLSADLSGYADLFRFAGMKMRATLLVYNLFDQLNDSWVN
                     GNTGRAYTAIIRETDLASHRSDFNTYEDRIHDPTMYSTPRLVKLGLEMVF"
     gene            complement(4458..5858)
                     /locus_tag="PKM23_08345"
     CDS             complement(4458..5858)
                     /locus_tag="PKM23_08345"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HNY91524.1"
                     /translation="MRSAALFRPVLRARFALLALILTLVIAPGCQRGPAPVDRAALAE
                     EVKTEFLHAWQGYKAHAWGHDELLPLSAGYRDWHSVSLLMTPVDAYDTMLLMGLTAEA
                     AEAKKLILDSLDFDHDITVKNFEITIRTLGGLLSAYQLDGDPRFLALAQELGDRLLPV
                     FNSPTGMPYTFVNLKTGAVSGPVSNPAEIGTLLLEFGTLSKLTGQKRYYNKAKIALVQ
                     LFNRRSDLGLVGTTINVETGEWGDTSSHISGMIDSYYEYLFKGWKLFGDGDCKLMFDS
                     SMVAVNRYLADEVEGCLWYGRADMNSGARGLRLFGSLDAFMPGLLIYAGDRSRAEKLQ
                     DSCYRLWMLHGIEPEMLDYGKMEVTAANYELRPENIESAWYLHRFTGEDKYLAMGRDY
                     FVSLRTHCRTEHGYAALASVITKEKKDSQESFFLTETLKYLYLLFAPVEIDLDQTVFN
                     TEAHPLKRTWPPDPVY"
     gene            complement(5887..6846)
                     /locus_tag="PKM23_08350"
     CDS             complement(5887..6846)
                     /locus_tag="PKM23_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HNY91525.1"
                     /translation="MQRHPANPVVTRQHVPAGTPHLTDVSSVFNPGAIQFHDQTILLL
                     RVQNRGRETFTLTARSRDGLHFTLSTPEIRFRGMEKLEERIYHCYDMRITPLEGRYYI
                     MFAMDMEEGCRLGLAVTDDFETFEFLGITSGGDTRNGVLFPERFNGLYLRLERPNRVQ
                     LAGGPASGSTIILSGSSDLLEWSPVGEVASGRFHYWDELIGGGPPPVKTRAGWLALYH
                     GVATHFASANIYQAGVMLLDLLDPCKVLARSRYNMLEPRESWELTGQVPNVVFPSGLI
                     VEEYDDQGFARFDSPFRLYYGAADTVVGLFTGRVSELIEAATA"
     gene            complement(6949..8142)
                     /locus_tag="PKM23_08355"
     CDS             complement(6949..8142)
                     /locus_tag="PKM23_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNY91526.1"
                     /translation="MKKIITGDYRLVKNLNSHIVLNLIRTRGPIYGADLAKITGMRPS
                     TIMSILRNLEKKGLIFKSGTGASSVQGGRKPTLWEICGNYGYIIGLQIELNEVHGVLI
                     DLNSHVLARSALPVEGKLTLEDLQSYIFRIVDTLLTPQKISRKNLLGMGIGVSGLVDY
                     RNGVIVNTALMGKVPVSLKAALDKHYDFPILVENDANAAAMCEKWYGKGKNISDLIYM
                     LLVMDQHVFGVGYGLYLEDQIYRGAHMFAGETAPQPISIKQMLEKVAPEYGGEVLLNG
                     RSLPKERVTLQDLIDAALNHDSLAIRYFQELAKIVARELAHIINLLDPEMILLGGEVS
                     KVGDLLLNAVRSELKADARARVSNDLDVKIETSTVVDAVAMGATTLILQNIFKSPVLQ
                     AKNYL"
BASE COUNT         1556 a         2264 c         2868 g         1732 t
ORIGIN      
        1 cggggagttg aggccggaaa gctcttcgta ggagtggagt tcggccatgg agatgcgttc
       61 ggtcagcccg cggggcagcc ggaaggtgcc cgagccgaag gtcatgacca ggttggccag
      121 ctcgccgaaa tactcctcga ggcgcggtga ggcgatctgc tcataggcct ccttgtcgaa
      181 ggccgagaag gggggctcat tctcctgggg atggtggagc atctggaagc cggtaaggcc
      241 gatcatgtcc gattcggaga tatcggtgag gccaaagttg ggttcgcagc cgaggccctc
      301 gacgaaatcg ggcttgtggt tgcattcgct tccatccgca tccgggccgg tatagttgag
      361 atcggtgggg gcgacaccgt cggtgccgat gtcattgccg aaatcctcac cgatgtcgta
      421 gacgccgttg ttgttggcat cggtgccgtc ctgccagtcc tggtcctcat cggcatccca
      481 gtgggttttg agctgctctt cggccaggcc gtaccaggtc aggaacttgg ccatgtcgct
      541 gattccctcg gtcgggccga tgaggcgggt ggcgacattg tcgcgtttct catcaacgag
      601 gccgtcgtca tcgctgtcga tgccgtcgtt ccagcggccg ggactttcga ggaaggccat
      661 gcccataatg ccggtgcgga tgccgccgat gccgatgccg tcatagtccc aggagtagga
      721 gatgtcttcg tacttgtcga aaaagccgat gtcgtccgtt tcgccctgat cgccgcgatg
      781 gccgatgccg gcgtcgagaa ggtagccgaa agcgacgtcg gcgaggtcat ggtcggagat
      841 attggagata tcatattccc aaaagatggc gtcgcgggtc tggggattgt tccactggta
      901 gccgcgcacc ttgacgcgga tgccgagacc gccccagggc tcgcccttct ggatggtgac
      961 atccggatcg ttatcgccga tcttgacgcc cgggcgcggg tagtacttga gcggggcatc
     1021 gggttgaaca tactcgagat cctgggcgtc gttggcgacg aaataggtct caaggtcggc
     1081 gtacttgacc ccgcgaccga aacggccgtc ccattcgccg ggccacttga gggtggagcc
     1141 cgccgagggc cagcccgctg caggccagga atcctcgcgg ttgctcatcg ccggatattc
     1201 gctgctctca ttgaaatagc cgaaaaccgg atagagcccc cagcggatga ggccgtcctt
     1261 ggagtggtcc atgcccatgc gatagctggt ctgacagaaa taaagctcct gcaaatcgct
     1321 gcggctggcg atttcggccg gatccgtgac gggggtggat ccgtccgcca tatgcaccct
     1381 ggcgacgatc cagagggcga tgccgtcatg catgccggtg ccggtgtagt cattgggcca
     1441 tttcgactgg tcaaagttgg gccagttggc cagctcggtg gtgttcttga aatagagcag
     1501 cacgcggttg ccgttcatcc agccctcgcg gatggcggcg atgtcgccgg ccggatcatc
     1561 cggaccctcg tatttgcgcc cctgccccat cagcagagcc ggaatgataa caagcaatag
     1621 caggaagcag gctacaggcg tagatttcat cacagatttt cctcgtatat gcatgatatc
     1681 cctctctcct gacctaaaaa accatctcga ggccgagctt gaccagacgc ggggtgctgt
     1741 acatggtcgg gtcgtggatg cggtcctcgt aggtgttgaa atcgctgcga tggctggcaa
     1801 ggtcggtttc gcggatgatc gccgtgtagg cgcgcccggt attgccattg acccaggaat
     1861 cgttgagctg gtcaaagaga ttgtagacca gcagggtggc gcgcatcttc attccggcga
     1921 aacggaagag atcggcatag ccgctcaggt cggcgctgag gctgctgggg atatgctcgt
     1981 tgttgggaaa gaggttgacc cgcgaaagcg ggctgtccgc gaggggactc caggtatagg
     2041 gtgaacccga gttataatag accgtcatgg tggcgccata gcggggcatg gaatagccca
     2101 cggtgacgtt ggcagtgtgg cgctgatccc agctcatcgg gatgagcttg ttgaccggat
     2161 cgcgctgcga accggcgcgg ctgaaggtgc tggtgggggt gtcggcgttg ccgcggctga
     2221 actgcagggt atagttggcc accaccgaaa gacgcccggc gatgtactca tacttgagct
     2281 cgaggccgcg cgcattggcg taatccttgt tgctgtagag gccgtagcgg atctggttgt
     2341 aggtggtgat gatcctggcg ctgagcagat cgtagatatc acggtaaaaa agcgccacct
     2401 cgaggctcat gtcgggcatc aactcctgcc agagcccgac ctcatactgg atggtcttct
     2461 gggcgttgac ctgggggttg cccatggtgg tcgaataatc gcccgggggg accaggcgcg
     2521 cgtaattgtc atacatcgca tagagcgggg gcatttgcag aaagtggccg tagccgaagc
     2581 gcagcagggc ggtcttgcca agctggtagg agatgcccag acgcggactg atctgatact
     2641 tggggtcggc ctcgacgggg tggctcatgc gctcgggatc accggggaag ctgagctggt
     2701 tggagggatt gcgccactgc gaaggatagg tgattttggg gtcgaaatag tcgaggcgga
     2761 gccccagatt gatcaccatc tcggcgaact ccatcttgtc ctggagatag gcgctgaatt
     2821 cgaccggttt gaccttgtag tactcatgat agagggtcga gtcgggcatg accatcggtt
     2881 cgtaaaagag atagcggcgc ttgccggtct cgggatcgac gtcccagagg gtctcgaagg
     2941 cggtgccgta atacttgttg cggatggtga accactcgtt ctcgagcact ttttgggagt
     3001 actgcgcgcc gccctcgatg ctgtgctggg gatggagctg ccagacaaaa tcgcccttgc
     3061 cggtatagtc ctcgagggtg cgttcgctgt ggcccttgct ttggccgccg gtggaaaagc
     3121 ccggcccctc gctgcggctg tagaggtcgt ggatgtagcg gctgtcgtag ggatccttga
     3181 acatatagct ggcattgttg tcggagacgt aggagccctt gagttcgtaa aaggccttgt
     3241 ccgagagcat gtggttccac tgcagcgagg ccaggtggga ggtgtgatag ccggtggagc
     3301 ggccgtcagg attgtatttc atgctgtggc tgtagccctg cccctcatcg tcgttgagga
     3361 tgtactgcag ggaggtcttg atcctggaac tcggccgcat ggtgagcttg ccccagacat
     3421 tgaggttttt ggcggtattc atggccacgt aggcgttgtc gccgctgtgc tcggtgtacc
     3481 actggctcga gtcggaggag gtgaagtcgc tgtaatcgtc gggcaggaag cggcggatgc
     3541 cattgaggtg ccccttgttg ttctggtagc gcacattggt ggcgaaggtg aatcggtcgc
     3601 cgatgatcgg gccgttgagg gcgaactggt agtcctgacg gcgggtgaaa tcggtgtcct
     3661 ggagcccgat aaagatatcc ttgtgggggg tgagatagtt gccgaggccg agagaggcgg
     3721 agccatggaa tttgcggccg ccgtccttgg tgacggcgtt gaccacgccg ctcatggcgt
     3781 tgccatattt ggcgctgaag atgccggtga tgacctcgag gtcctcgacg acgtcttttt
     3841 cgacgttgac ggtgcggccg ccgcggccgt aggattcgtc cacctgcatg ccgtcgatga
     3901 gataggtgac ctcatcgctg cggccgccgc ggaaatggcc gagcaccacg cccggctgca
     3961 tggcgaccac cgcgctcacc gattcggtgg gcaggatatc catctgggtg gaggagacgt
     4021 tgcgcaccga actggtctgg tccttcttga ccgcgacgcg gtcggcgacg acagtgacct
     4081 cctggccgct gatgacggtg ctcttgagtt tggcggtgag ctcgaaggtg cggttgaccg
     4141 agacacgcac gttctgcatg cgcagggtct cgtagccgac catcgagacg cgcacggtgt
     4201 aggtgccggg ctcgaggttg atgatgaaga attcaccgtt gatgtccgcc gtggcaccgc
     4261 ggctggtccc ctccaggagg atgttggcgc cggtgagggt ttccccgctg gtgagatcgg
     4321 agaccctgcc ggcgatcttg ccggtggtct gacccagcag cggggctgcg gtgagaatca
     4381 ggcagaggag tgcggccgtc aggtatttgg ccatgataca tttccttttc tggtgtacag
     4441 gtgtcaaagt ggtgcgctca atagacggga tccggaggcc aggttcgctt gagaggatgg
     4501 gcctcggtat tgaagacggt ctggtcgaga tcgatctcca ccggcgcgaa gagcagatag
     4561 aggtacttca gggtctcggt gaggaaaaag ctctcctggg aatccttctt ctctttggtg
     4621 atgacgctgg cgagagcggc gtagccgtgt tcggtgcggc agtgggtgcg cagggagaca
     4681 aaatagtccc ggcccatggc gagatacttg tcctcgccgg tgaagcggtg gaggtaccag
     4741 gcagattcga tgttctcggg ccgcagctcg taattggcgg ccgtcacctc cattttgcca
     4801 tagtcgagca tctctggttc gatgccgtgc agcatccaga ggcggtagca ggagtcctgc
     4861 agcttttcgg cgcggctgcg gtcgccggca tagatgagca ggccgggcat gaaggcgtcg
     4921 aggctgccga aaaggcgcag ccccctggcg ccgctgttca tgtcggcgcg gccgtaccag
     4981 agacacccct cgacctcgtc ggcgaggtag cggttgacgg cgaccatgct cgaatcgaac
     5041 atgagcttgc agtcgccgtc cccgaagagc ttccagccct tgaagagata ctcatagtag
     5101 gagtcgatca tgccgctgat gtggctggag gtatcccccc actcgccggt ctcgacgtta
     5161 atggtggtgc cgaccaggcc gagatcggag cggcggttga agagctggac cagggcgatc
     5221 ttggccttgt tgtagtagcg cttttggccg gtcagtttgc tgagagtgcc gaactcaagc
     5281 agcagggtgc cgatttcggc cggattggag accggcccgc tcactgcgcc ggttttgaga
     5341 ttgacgaagg tgtagggcat gccggtgggg gaattgaaga cggggagcag gcggtcgccc
     5401 agctcctgcg ccagggcgag aaagcgggga tcgccgtcga gctggtaggc ggagagcagc
     5461 cccccgagcg tccggatggt gatttcgaaa ttcttgacgg tgatatcgtg gtcaaagtcg
     5521 aggctgtcga ggatgagttt tttcgcctcg gccgcttcgg cagtgagccc cattaacagc
     5581 atggtgtcgt aggcatcgac cggagtcatg agcagggaga cgctgtgcca gtcgcgatat
     5641 cccgcgctga ggggcaagag ttcgtcatgg ccccaggcgt gggccttgta gccctgccag
     5701 gcgtggagga actcggtttt cacctcctcg gccagggcgg cgcggtcgac cggagccggc
     5761 ccgcgctggc agccgggggc gatgacaagg gtgaggatga gcgccaggag agcgaaccgg
     5821 gcgcgaagaa cgggacggaa caaagctgcg gatctcaatg cacgcctcct tgaagaacca
     5881 tggatgtcag gccgtggcgg cctcgatgag ttcggagacc ctgccggtga agagccccac
     5941 cacggtatca gccgcgccgt agtaaaggcg gaagggcgag tcgaaacggg cgaagccctg
     6001 gtcgtcatac tcctcgacga tcaggccgga ggggaaaacg acgttgggga cctggccggt
     6061 gagctcccag ctctcgcgcg gctcgagcat gttgtagcgg ctgcgtgcga gcaccttgca
     6121 ggggtccagc agatcgagga gcatcacccc ggcctgatag atgttggcgc tggcgaagtg
     6181 ggtggcgacg ccgtgataga gggccagcca gccggcgcgg gtcttgaccg ggggcgggcc
     6241 gccgccgatg agttcatccc agtaatgaaa gcgcccggag gccacctcgc cgacaggact
     6301 ccattcgagg agatcggagg aaccggagag gatgatggtg ctgccgctgg cgggtcctcc
     6361 ggcgagctgg acgcggttgg gacgctcgag gcggagatag aggccgttga agcgttcggg
     6421 aaagagcacc ccgttgcggg tgtcgccgcc gctggtgatg ccgaggaact cgaaggtttc
     6481 aaaatcgtcg gtgacggcga ggccgaggcg gcagccctcc tccatgtcca tggcgaacat
     6541 gatataatag cggccctcga ggggggtgat gcgcatgtca tagcagtgat agatgcgctc
     6601 ctcgagcttt tccataccgc ggaagcggat ctccggtgtg gagagggtga aatggaggcc
     6661 gtcgcggctg cgggcggtga gggtgaaggt ttcgcggccg cggttctgga cgcggaggag
     6721 gaggatggtc tggtcgtgaa actggatggc gccgggattg aagaccgagg agacgtcggt
     6781 caggtggggg gtgccggccg gcacgtgctg gcgggtgacc accggattgg cgggatggcg
     6841 ctgcatcgct tcctcccttg atatgttgtt agtttttttg ttgtaaaaaa tagaaagggc
     6901 gtcaaaaacg cccggtgcaa cccgggcgtc ccgcacacct gccccgcctt acagataatt
     6961 cttcgcctgc agcaccggac tcttgaagat gttctgcaga atcagcgtcg tcgcccccat
     7021 cgccaccgca tccaccaccg tcgaggtctc gatcttgaca tcgagatcat tgctcacccg
     7081 agcccgggca tccgccttga gctcgctgcg cacggcattc aggagcagat cgcccacctt
     7141 gctcacctct ccgccgagca ggatcatctc gggatcgagc agattgatga tgtgcgccag
     7201 ctcccgggcg acgatcttgg ccagctcctg gaaatagcga atggccaggg agtcgtggtt
     7261 cagggccgcg tcaatgaggt cctgcagggt cacccgctcc ttgggcaggg agcggccgtt
     7321 gagcagcacc tcccccccgt actccggcgc caccttttcg agcatctgct tgatcgagat
     7381 cggctggggc gcggtctcac cggcgaacat gtgcgccccg cgatagatct gatcctcgag
     7441 gtaaaggccg tagcccaccc cgaaaacatg ctggtccatg accagcagca tatagatcag
     7501 atcgctgata ttcttgcctt tgccatacca cttttcgcac atggcggcgg cattggcgtc
     7561 attctcgacg aggatgggaa aatcgtagtg tttatccagg gccgccttga gggagaccgg
     7621 aaccttgccc atcagggcgg tgttgacgat caccccgttg cggtaatcga ccagccccga
     7681 gaccccgatg cccatgccga gcaggttctt gcggctgatc ttctgggggg tgagcagggt
     7741 atcaacgatg cggaagatgt aggactggag atcctccagg gtcagcttgc cctcgacggg
     7801 cagggcgctg cgcgcgagca catgggagtt gaggtcgatg agcaccccgt gcacctcgtt
     7861 gagctcgatc tgcaggccga tgatgtagcc gtaattgccg cagatctccc agagggtggg
     7921 cttgcgtccc ccctgcaccg aggaagcgcc ggttcccgac ttgaagatca atcccttctt
     7981 ttccaggttg cggaggatgc tcatgatcgt cgaagggcgc atgccggtga ttttggccag
     8041 atccgcacca tagatgggcc cgcgcgtccg gatgagattg aggacgatgt ggctgttgag
     8101 gttcttgacc agccggtaat cgcccgtgat gatttttttc atggcctcac aatgcccttc
     8161 tgcctgttcc ggtccggcgc tttaagagct gacggggtcg cctggaaatt cgtgatccga
     8221 tccgaatatc aattattttt attggaaaaa taactcgtaa atatattcct ttttttgtaa
     8281 caaatcaaca ctcttttttg attttttaag agcccctccc ccctggtgca ggcataataa
     8341 agcccgcgtg gtggctggca cgcgggccgg agaatcggac cgtcacaggg gggaaccggc
     8401 gccgatgaaa gaaagtgcgg
//
LOCUS       DAOPWU010000137         8386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8482_length_8386_cov_4.808306, whole genome shotgun sequence.
ACCESSION   DAOPWU010000137 DAOPWU010000000
VERSION     DAOPWU010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8482_length_8386_cov_4.808306"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PKM23_08360"
     CDS             <1..1091
                     /locus_tag="PKM23_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854712.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNY91527.1"
                     /translation="EMFNLWKPLVPHGVQGTWAPVMETGRMAWYFNNNYPWIGMLFCA
                     PIVGLWYWCTDQYIVQRTLSASSETEARRGSIAAAFFKLLPVFIFIIPGIIAFALAKS
                     GQNATLQAAMMDANGNLIRDNAQSAFPLLVANILPVGVRGMVVAGLLAALMSSLAGAF
                     NASSTLFTMDFYSRLRPNVSQERLVWIGRVATGVMVLIGLLWIPVIRGGRGLYDYLQG
                     VQGYLAPPIFVVFFLGVFNKRMNAKGAMAALITGFSLGIIRLAVDTPPKLIQGFSYAT
                     GSFLWVFNNIFFQYYSILIAVICAAVMLIVSYRTSEPDYARISGLTYGTVTEAHRKAT
                     RASWGRGDLMASILVICLILAAYLYFVG"
     gene            1107..2789
                     /locus_tag="PKM23_08365"
     CDS             1107..2789
                     /locus_tag="PKM23_08365"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HNY91528.1"
                     /translation="MPEREACVIGLDYGTDSVRALIVRAADGAEIATDVFYYPRWKQG
                     LYCDAAANRFRQHPLDYVEGLEAVIRGALDRAPAGTAARVRGISVDTTGSTPCAIDRQ
                     GSPLALKPEFANNPNAMFVLWKDHTAIREAGEINTLAKSWGGIDYTRYEGGVYSSEWF
                     WSKILHVLREDEEVREAAFSWAEQCDWIPALLTGMSDPRTWKRSRCAAGHKAMWHESW
                     GGLPAEEFLVRLDPLLTGLRARLYRDTFTGDRAAGVLSPAWAQRLGLADGVVVGVGAF
                     DAHFGAVGAGIQPHMLVKVMGTSTCDMITTPLTGGEEHLVAGICGQVDGSILPGQLGL
                     EAGQSGFGDIYAWFKELLLWPMEHLGETLVPGEAAERQRRIEEAAEALIPALSEAAAA
                     LPIEESGVLAVDWMNGRRTPDANQALKGAIAGLNLGSDAPRVFRALVEATAFGARAIV
                     DRFIAEGVDIRGVIGIGGVAKKSPFIMQIMADVLNMPIRVAAAEQACALGSAMAAAAA
                     AGLHPDLGSAQKAMGSGFETVYTPDAGNAEKYRVLYSRYLKICRFIEEEFTE"
     gene            2875..4356
                     /locus_tag="PKM23_08370"
     CDS             2875..4356
                     /locus_tag="PKM23_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HNY91529.1"
                     /translation="MKNVPVIKLGLVAVSRDCFPIELSHSRRSKVAAACKALELPLVE
                     IPTIIENENHTLQALEELKKNGVNALVVYLGNFGPEGPETILAQRFDGPVMFVAAAEE
                     SGSDLIDGRGDAYCGMLNASYNIGLRKLRPWIPEYPVGTPEEVAALIRDFLPVARIIV
                     GLKHLKIFSFGPRPQDFLACNAPIKPLYDLGIEIMENSELDLYDICLAAKEDPMVAEV
                     SRAMADELGVGNTYPGMLDKLAQYEVALRRFMENNLGASRYGIFANKCWPAFEKYFGH
                     VPCFINSRLAAEGIPVSCEVDIYGALSEYIAACATLRPPAILDINNTVPRDMYEANRA
                     AMKDYAQSDLFMGFHCGNVSSACLVRPKMKYQLIMHRLQEPGQEPNITRGTIEGQIRA
                     GEVTIFRLQSTADTRLKSYIAQGEVLDVDPRSFGSIGVFAIREMGRFYRHVLIEKRYP
                     HHTAVAFAHAGKALYAAARMLGVGDIAFNQPRGVYYPTENPFA"
     gene            4388..5530
                     /locus_tag="PKM23_08375"
     CDS             4388..5530
                     /locus_tag="PKM23_08375"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNY91530.1"
                     /translation="MKRSVILIIALAAALLLTLGSACNKPGEAEGPSISSAPFGQTAE
                     GQAVALYTLKNSRGTTVKITPWGGVVTTIIVPDRNGTMGDVALGFDALAGYTDAVPYF
                     GALIGRYGNRIAKGAFALDGTVYTLPVNNGANHLHGGPRGFDKVLWTAGPVQPGKPSL
                     TLTYVSKDGEAGYPGNLTATVIYTLTEADELEIDYTAITDKKTVVNLTNHTYFNLAGV
                     GDILAHEVQIDADRYTPVDDGLIPTGELAPVDNTPFDFRTPTAIGARINDEDVQIKRG
                     NGYDHNWVLNGAMGSMRKVAAVFEPLSGRVLEVLTTEPGLQFYTGNFLDGTLTGKAGQ
                     VYGFRSGFCMETQHYPDSPNQPAFPSTVLNPGETYKTRTIYKFTTR"
     gene            complement(5599..7518)
                     /locus_tag="PKM23_08380"
     CDS             complement(5599..7518)
                     /locus_tag="PKM23_08380"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNY91531.1"
                     /translation="MNVTLSAAGNRIYYTLDGSDPDSASALYTGPVSVAKTAVLKAVS
                     VETGHQPSRCAHATYFINETSKLPVVSLTSDPANLFDYNTGIYADGPGWTPAAPHHGA
                     NYWMDWEKPAHVEFFDDDKKLGFSEECGIAIYGAYTRSFPQKSISVKFKSDYGVNGID
                     YPLFPDLALTRHKAFVLRNSGNDFQYTHFRDAMMQTLIQDLDIDYLEYRPANAFINGE
                     YWGVYNIREKVNEHYIANHHGVDPDNIDMLEGNMEVIHGDTQNYQELIDYLSTRDMTS
                     DAAWEYVNRMIDLDECLLYWAAEVYYNSQDWPANNVKYWRERSENGKWRWILFDLDFG
                     FNLYETTGQSENHLYYLLSGIETRPGSNPPWSTLLPRKLVENPRIKNQFINLIADLLN
                     THFESTRVVNTINEMAAAITPDIARHRKRWGISEKTASDHVKRMTTFAQERPNYLRGF
                     VRDFFKCGSPGTLTLNTTTGGRVRLNSLTFTADKLPWSGSYFTGNPIHLTAIPDPGWK
                     FAGWSGSSASTDTSLSLLVGRNSSLTATFAAISSGVEAAGNALPQRLELLPNYPNPFN
                     PSTTIAFVLPAAGPVSLRIHDLLGREVALLISGSMPAGAHKVQWDAGGAAGGIYFCRL
                     EAGGTAQSRKLILMR"
     gene            complement(7528..8058)
                     /locus_tag="PKM23_08385"
     CDS             complement(7528..8058)
                     /locus_tag="PKM23_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNY91532.1"
                     /translation="MYRHWLFLLLLIPALLSAQSVVINEIVASNQKTIDDEDGDASDY
                     IELYNSGAAALQLEGYGLSDDPKTVKWHFGAALLAPGEHLIVFASDKDKKGVYWHTNF
                     KISASGETVVLTDAAGAVVDRIDVPTSSGDIAWGRTGDGASAWAFQTPSPGAANIGKI
                     ILPYADPVTVSPPAGF"
BASE COUNT         1472 a         2627 c         2658 g         1629 t
ORIGIN      
        1 ccgagatgtt caacctctgg aagcccctgg tgccgcacgg cgtgcagggg acctgggcgc
       61 cggtgatgga gacgggccgc atggcctggt atttcaacaa caactatccc tggatcggca
      121 tgctcttttg cgccccgatc gtcggcctct ggtactggtg caccgaccag tacattgtcc
      181 agcgcacact gagcgccagc agcgagaccg aagcccgtcg cggttcgatc gcggccgcct
      241 ttttcaagct cctcccggtc ttcatcttca tcatccccgg catcattgcc ttcgccctgg
      301 ccaagagcgg ccagaacgcc accctgcagg cggcgatgat ggatgccaat ggcaacctca
      361 tccgcgacaa cgcccagagc gccttcccgc ttctggtcgc caatatcctt cccgtcggcg
      421 tgcgcggcat ggtggtggcg ggactcctgg ccgccctgat gagctccctg gcgggggctt
      481 tcaatgccag ctcgaccctc ttcaccatgg atttctactc ccggctgcgg cccaacgtca
      541 gccaggagcg gctggtctgg atcggccgcg tcgccaccgg cgtcatggtg ctcatcggcc
      601 tgctctggat cccggtcatt cgcggcggcc ggggcctcta tgattacctc cagggggtgc
      661 agggctatct cgctccgccc atctttgtcg tctttttcct cggcgtcttt aacaagcgca
      721 tgaacgccaa aggggcaatg gccgcgctca tcaccggttt cagcctcggc atcatccgcc
      781 tcgcggtcga tacgccgccc aaactgatcc agggcttcag ctatgccacc ggctccttcc
      841 tctgggtttt caacaatatc tttttccagt actacagcat cctgatcgcg gtgatctgcg
      901 ccgctgtgat gctgatcgtg agttatcgca cctccgagcc cgattacgct cgcatcagcg
      961 gcctgactta cggtacagtc accgaggcgc accgcaaggc gacccgcgcc agctggggcc
     1021 ggggcgacct tatggcttcg atcctggtca tctgcctgat cctggccgcc tacctctact
     1081 tcgtcggctg agagcggaga aaaaccatgc ctgaaagaga ggcctgcgtc atcggactgg
     1141 attacggcac cgattcggtc cgcgccctga tcgtacgcgc cgccgacggg gccgagatcg
     1201 caacggatgt tttttattat ccgcgctgga agcagggcct ctactgcgat gcggccgcga
     1261 accgcttccg tcagcatccc ctcgattatg tcgaggggct cgaggcggtc atccgggggg
     1321 ccctggacag ggccccggcc ggcaccgcgg cgcgcgtgcg gggcatctcg gtcgatacca
     1381 ccggctcaac cccctgcgcc atcgaccggc aggggagccc cctggccctg aagcccgaat
     1441 tcgccaacaa tcccaacgcg atgttcgtgc tctggaagga ccataccgcc atccgcgagg
     1501 ccggggagat caacaccctc gccaaaagct gggggggcat cgactatacc cgctacgagg
     1561 gcggggtcta ctcctccgaa tggttctggt ccaaaattct ccatgtgctg cgcgaggatg
     1621 aggaggtgcg cgaggcggct ttttcgtggg ccgagcagtg cgactggatt ccggcgcttt
     1681 tgaccggcat gagcgatccc cgcacctgga agcgcagccg ctgcgccgcg ggccacaagg
     1741 cgatgtggca tgaatcgtgg gggggactgc ccgccgagga gttcctggtc cgtctcgacc
     1801 ccctgctgac cggcttgcgg gcccggctct atcgcgacac cttcaccggc gaccgggccg
     1861 ccggcgtgct ctcgcccgca tgggcgcagc ggctcggact ggccgacggt gtcgtcgtcg
     1921 gggtcggcgc cttcgacgcc cacttcgggg ccgtcggggc cggtatccag ccccacatgc
     1981 tcgtcaaggt gatggggacc tcgacctgcg acatgatcac cacccccctg accgggggcg
     2041 aggagcatct tgtggccggc atttgcggtc aggtggacgg ctcgatcctt cccggccagc
     2101 tcggcctcga ggccggacag tccggtttcg gcgatatcta cgcctggttc aaggaactgc
     2161 tcctgtggcc gatggagcac ctcggggaaa cccttgtacc gggcgaagcg gcggagcggc
     2221 agcggcggat cgaggaggcg gccgaggcgc ttatccccgc gctctcggaa gcggctgcgg
     2281 ctctgcccat tgaagagtcg ggggttcttg ccgtggactg gatgaacggc cggcgcaccc
     2341 cggatgccaa tcaggccctc aagggggcca tcgccggcct caacctcggc tcggatgcgc
     2401 cccgcgtctt ccgcgccctc gtcgaggcta cggccttcgg cgccagggcc atcgtcgacc
     2461 gcttcatcgc cgaaggggtc gacatcagag gcgtcatcgg catcggggga gtggcgaaga
     2521 aatccccctt catcatgcag atcatggcgg acgtgctcaa catgcccatc agggtggctg
     2581 cagcggaaca ggcctgcgct ctgggttccg ccatggcggc tgctgcggca gccggtctcc
     2641 atcccgatct gggctccgcc cagaaagcga tggggagcgg cttcgagacc gtctataccc
     2701 cggacgccgg caacgcggag aagtaccggg ttctctacag ccgctacctg aagatctgcc
     2761 gttttatcga agaggaattc actgaataac ccgccggcag gaggtgtcac cggaaccatc
     2821 cggagggctg cggtacgggt ccggatcccc tgcggccaga aggagtgcct ggagatgaag
     2881 aatgtccccg tgatcaaact cggcctcgtc gcagtcagcc gtgactgctt ccccatcgag
     2941 ctctcccatt cgcggcggag caaggtggcg gcggcgtgca aggcgctgga gctgccgctg
     3001 gttgagatcc cgaccatcat cgagaatgaa aatcataccc tgcaggctct tgaggagctg
     3061 aaaaagaacg gggtcaacgc tttggtggtc tatctcggca actttggtcc tgagggaccc
     3121 gagaccatcc ttgcccagcg cttcgacggc ccggtgatgt tcgtcgccgc cgccgaggag
     3181 agcggcagtg acctcatcga cggccgtggc gacgcctact gcggcatgct caacgcctcc
     3241 tacaacatcg gcttgcgcaa gctgcgcccc tggatccccg agtatcccgt cggcacgccg
     3301 gaggaggtag ccgcgctgat ccgggatttc ctgccggtgg cgcgcatcat cgtcggcctg
     3361 aagcatctga agatattctc tttcgggccg cggccgcagg atttcctcgc ctgcaacgcc
     3421 ccgatcaagc ccctctacga cctcggcatc gagatcatgg agaacagcga gctggatctt
     3481 tacgatatct gcctcgccgc caaagaggac ccgatggtgg cggaggtcag ccgggccatg
     3541 gccgatgagc tgggcgtggg caacacctat cccggcatgc ttgacaagct ggcgcagtat
     3601 gaggtcgccc tccgccgctt catggagaac aacctcggcg ccagccgcta cggcattttc
     3661 gccaacaagt gctggccggc ctttgaaaag tatttcggcc acgtcccctg cttcatcaat
     3721 tcgcgcctgg ccgccgaggg gatcccggtc tcctgcgagg tcgatattta cggcgctctg
     3781 agcgagtaca tcgccgcctg cgccacgctt cgcccgccgg ccatcctgga tatcaacaac
     3841 actgtgcccc gggacatgta tgaggccaac cgtgccgcca tgaaggatta tgcccaaagt
     3901 gaccttttta tgggcttcca ctgcggcaac gtctcctcgg cctgcctggt ccggccgaag
     3961 atgaagtatc agctcatcat gcaccgcctg caggagccgg ggcaggagcc caatatcacc
     4021 cgcggcacca tcgaggggca gatcagggcc ggcgaggtga ccattttccg cctccagtcc
     4081 acggccgata cccgcctcaa gtcttatatc gcccagggcg aggtgctcga tgtcgatccg
     4141 cgctccttcg gcagtatcgg cgtcttcgcc atccgggaga tgggccgctt ttaccgtcat
     4201 gtcctgatcg aaaagcgcta tccccaccac acggcggtcg cctttgccca tgccggcaaa
     4261 gccctctatg ccgcggcgcg catgctcggc gtcggggata tcgccttcaa tcagccccgg
     4321 ggagtctatt atcctacgga gaatcccttt gcctgagggc agggagaatg cataacaagg
     4381 agaagtgatg aaacgcagtg taatcctgat catcgccctg gcggcggcgc tcctgctgac
     4441 gctgggttcc gcctgcaaca agcccggcga ggctgaaggg ccttccattt cttccgcccc
     4501 cttcggtcag accgccgaag gccaggcagt cgctctttat accctgaaga acagccgcgg
     4561 caccaccgtc aagatcaccc cctggggcgg ggtcgtcacc accatcatcg tgcccgaccg
     4621 caacggcacg atgggcgatg tggcgctcgg ctttgacgcg ctcgcaggct acaccgacgc
     4681 ggtcccctat ttcggcgccc tcatcggccg ctatggcaac cgcatcgcca aaggcgcctt
     4741 cgcgctggac ggcacggtct acaccctgcc ggtcaataac ggcgccaacc acctccacgg
     4801 cggccccagg ggctttgaca aggtgctctg gacggcggga ccggtccagc ccggcaagcc
     4861 aagcctcact ctcacctatg tgagcaagga cggggaggcg ggctatcccg gcaacctcac
     4921 cgcgacggtg atctacaccc tgaccgaagc ggatgagctg gagatcgact ataccgcaat
     4981 cactgataaa aagacggtgg tcaacctgac caatcacacc tatttcaacc tcgccggggt
     5041 gggcgacatc ctcgcccatg aggtgcagat cgatgccgac cgctacactc cggtcgacga
     5101 tggcctcatc ccgaccggag aactggcgcc ggtcgacaac accccctttg atttccgcac
     5161 accgactgcg atcggggcgc gcatcaatga cgaggatgtg cagatcaaac gcggcaacgg
     5221 ctatgaccac aactgggtgc tgaacggcgc gatggggagc atgcgcaagg tggctgcggt
     5281 gttcgagccc ctcagcggca gggtgcttga ggtcctcacc accgagcccg ggctgcagtt
     5341 ctataccggc aactttctcg atggcactct caccggcaag gccggccagg tctacggctt
     5401 tcgctccggc ttttgtatgg agacccagca ctatcccgat tcgcccaacc agcccgcttt
     5461 cccctccacg gtgctcaatc ccggcgagac ctacaagacc cgcaccatct acaaatttac
     5521 gacccgttag gaaatctgca caggcaaggc cacccctgag ggcggcagtg ggatcctctt
     5581 ccgatcccgc tgccgccctc agcgcatcag gatgagcttt ctgctctgag ctgttccccc
     5641 cgcctcgagc cggcagaagt agattccgcc tgctgctccg ccagcgtccc actgcacctt
     5701 gtgtgcgccg gccggcatgc tgccggaaat cagcagggcg acctcccgtc cgagcaggtc
     5761 gtggatcctg agtgagaccg gtccggccgc gggcaggacg aaggcgatgg tggtggaggg
     5821 attgaaggga ttggggtaat tgggcaggag ttccagccgc tgcggaaggg cgttccccgc
     5881 ggcctcgacc ccgctgctga tggcggcgaa agtggccgtc aggctcgagt ttcggccgac
     5941 gagcaggctc aggctggtgt cggtggaggc ggaggagccg ctccagccgg cgaatttcca
     6001 gcccggatcc gggatagcgg tgagatggat gggattgccg gtgaaatagc tgcccgacca
     6061 gggcagcttg tccgccgtga aggtgaggga gttgagccgc acccgccccc cggttgtggt
     6121 gttgagcgtg agggtgcccg ggctgccgca tttgaagaaa tcgcggacaa aaccgcgaag
     6181 atagttcggc cgctcctggg cgaaggtggt catgcgcttg acgtggtcgc tggcggtctt
     6241 ttcgctgatc ccccagcgct tccggtgccg ggcgatatcc ggggtgatcg ccgcggccat
     6301 ctcgttgatg gtattgacca cccgggtgct ctcgaagtgg gtattgagca gatcggcgat
     6361 gagattgatg aactggtttt tgatccgggg attctcgacc agcttgcgcg gcagcagggt
     6421 ggaccagggg ggattggagc cggggcgggt ctcgatgccc gaaagcaggt agtagaggtg
     6481 gttctcggac tggccggtgg tctcgtacag attgaagccg aaatcgaggt cgaagaggat
     6541 ccagcgccat ttgccgttct cgctgcgttc gcgccagtac ttgacattgt tagcgggcca
     6601 gtcctggctg ttgtaataga cctccgccgc ccagtagagc agacattcgt ccagatcgat
     6661 catgcggttg acatactccc aggctgcgtc gctggtcatg tcccgggttg agaggtaatc
     6721 gatcagctcc tgatagtttt gggtgtcgcc gtggatcacc tccatgttgc cctcgagcat
     6781 gtcgatgttg tcgggatcga cgccgtggtg gttggcgatg taatgttcgt tgaccttttc
     6841 gcggatgttg tagacgcccc agtattcgcc gttaataaag gcgttggcgg gccggtactc
     6901 cagatagtcg atgtcgaggt cctgaatcag ggtctgcatc atggcgtcgc ggaagtgggt
     6961 atactgaaaa tcgttgcccg agttgcgcag cacaaaggcc ttgtgccttg tcagcgccag
     7021 gtcggggaag agcggatagt cgatgccgtt gacgccgtag tcgctcttga attttaccga
     7081 gatcgatttc tgggggaaag agcgggtata ggcgccgtag atggcgatgc cgcactcctc
     7141 cgaaaaaccg agctttttgt catcatcgaa gaactcgaca tgggccggct tttcccagtc
     7201 catccagtag ttcgcgccat ggtggggggc cgccggcgtc cagccgggtc cgtcggcgta
     7261 gatgccggtg ttataatcga agaggttggc gggatcgctg gtcagtgaga cgaccggcag
     7321 cttgctggtt tcgttgatga aataggtggc gtgagcgcac cgggagggtt ggtggccggt
     7381 ttcgacgctc accgccttga gcaccgcggt tttcgccaca ctcaccgggc cggtgtagag
     7441 cgccgaggcg gagtcgggat cgctgccgtc cagggtgtag tagatgcggt tacccgcggc
     7501 ggagagggtg acgttcacag ccgcaggtta aaagcccgcc ggcggggaga ctgtcaccgg
     7561 gtcggcgtag gggaggatga ttttgccgat gttggcagcg ccgggcgagg gcgtctggaa
     7621 ggcccacgcg gaggcgccgt cgccggtgcg gccccaggcg atgtccccgc tcgaggtcgg
     7681 cacatcgatg cggtcgacga ccgcaccggc ggcgtcggtc agcacgacgg tttcgcccga
     7741 ggcgctgatc ttgaaattgg tatgccagta aacgcccttt ttgtccttgt ccgaggcgaa
     7801 gacgatgaga tgttcgccgg gggccagaag ggcggcgccg aaatgccact tgacggtttt
     7861 gggatcgtcg ctcagtccgt agccctcgag ctggagcgct gcggcgccgc tgttatagag
     7921 ctcgatatag tcggaggcat ctccgtcttc atcgtcgatc gtcttttggt tggaggcgac
     7981 gatttcgttg atcaccacgc tctgcgcaga aaggagagcg gggatgagca ggagcaggaa
     8041 gagccagtgt ctgtacatgg gggaactccg tcggctgatc agggagagag tacatgatgc
     8101 aggggcacca ctgtgaacag gggaagttac gcagatgcac ctccttatgc aatatttttc
     8161 ttctccctct cccggcggaa cgctactcct ttttgcttga atttggccgc tttaatggct
     8221 aacttggctg cgagcggtca tcatggtttc ggccgccggg ggtgatttgc atttcagtga
     8281 actttcggag agtgtatgca aaaagtgctg ctgtattcta tcatcctgct gagcctggcg
     8341 gcctccctgc ccctcgccgc ccaggagatg cccctggtct acgatg
//
LOCUS       DAOPWU010000138         8369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8502_length_8369_cov_5.387058, whole genome shotgun sequence.
ACCESSION   DAOPWU010000138 DAOPWU010000000
VERSION     DAOPWU010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8369
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8502_length_8369_cov_5.387058"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1142
                     /gene="gap"
                     /locus_tag="PKM23_08390"
     CDS             135..1142
                     /gene="gap"
                     /locus_tag="PKM23_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNY91533.1"
                     /translation="MAIRMGINGFGRIGRLVFRAAVQRKDIEIVQVNDITDAPTLAHL
                     LKYDSVHGVLKNDIRAEGEYIVFDGKKVKVSAEKDPANLPWGKENIDIVVESTGVFRK
                     RAEVAKHLEGGAKKVILTVPAKDEIDATIVIGVNDEALKPEHKVVSNASCTTNCLAPV
                     AKVLHDAFGIKRGFMTTVHAYTNDQRILDLPHKDLRRARAAAESIIPTTTGAAKAVGK
                     VIPELKGKLDGFSLRVPVSDGSVVDLVAELNKDVTIEQVNAAIKAAANGKLKGILEYT
                     EDPIVSIDIVGNPASSIFDALSTMVLEGTSNFVKVVSWYDNEWGYSCRVVDLVEKMGK
                     M"
     gene            1235..1984
                     /gene="tpiA"
                     /locus_tag="PKM23_08395"
     CDS             1235..1984
                     /gene="tpiA"
                     /locus_tag="PKM23_08395"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNY91534.1"
                     /translation="MRKRIIAGNWKMNKSVTEAAELARAVVEGMKTATETEVVLCPPF
                     TDLTAVAAVIQGTPVGLGAQNLHWEEKGAYTGEVSAAMLLSAGCTHVIIGHSERRQYF
                     GETDESVNRKVKTALKAGLIPIVCVGETLAERQADETAAVVERQVRAALEGLTDAQVA
                     GLVIAYEPVWAIGTGVVATTEQAQEVHALIRALVGKLCSERVAAALRIQYGGSMKPDN
                     AGQLLAQKDIDGGLIGGASLDARSFLDIVYA"
     gene            2006..2350
                     /gene="secG"
                     /locus_tag="PKM23_08400"
     CDS             2006..2350
                     /gene="secG"
                     /locus_tag="PKM23_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNY91535.1"
                     /translation="MYTVLIILFVLVCILLTFVILIQSSKGGGLAGSFGGMDTMGTLF
                     GGGRGSAPFLQKLTMILAAVFLAIALVMGFMTRGGVSTQSLVEQERDRLNSSPARTLP
                     QIPNQPAQTPGK"
     gene            2383..2468
                     /locus_tag="PKM23_08405"
     tRNA            2383..2468
                     /locus_tag="PKM23_08405"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2417..2419,aa:Leu,seq:gag)
     gene            2572..3486
                     /locus_tag="PKM23_08410"
     CDS             2572..3486
                     /locus_tag="PKM23_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNY91536.1"
                     /translation="MMADYTRRDFIRIGAGAGAALAALPMLPEGLLAVPNPDLAVARN
                     GAPGLLVRKAVEAIGGMGRFVKKGQTVLLKPNMGWDRLPEQAANTNPEAVAEVVRMCL
                     EAGASRVRVLDRTCNEPRRCYMRSGIEKAAKAAGAEVRHIVDSRFQDLAISGGELLTS
                     WPVYRDALESDVIINMPIAKHHNISGVTLSFKNLMGLLGGDRGALHNHFMTKIVDINM
                     AIRPSLTIIDAWRIMLRNGPTGGSLADVAEKQLFIASADRVAADAWAMGLFNIDPNTV
                     EYLRIAAKRGLGQLDLKRVKIQEINLGV"
     gene            3555..5114
                     /locus_tag="PKM23_08415"
     CDS             3555..5114
                     /locus_tag="PKM23_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY91537.1"
                     /translation="MMITLRRISQLLFLSLFVLLFFQARFPYEAWLPADLFLRASPLV
                     ALTVMIASRAFIASTILALIVLALTIPLGRFFCGWVCPLGTIIDGSDHLIRRKRRPAR
                     QRDPLTWRSWKFFLLAALLVAALFSTQFIWFFDPIALLTRTFTTVLHPAFSFLLGGLF
                     NAAFGLHLFEDQVYSLYDAAQAGGLMPVQQPRLYENMAIALIFAAILGLGMVSRRFWC
                     RNLCPLGALLGIFSKYRLTRRHVSAACTKCSICQRECKMNAIEDDYSINNTVECIECA
                     ECVSVCKPRAVSYTIGRSRGENRIDLSRRRLLQASAAGAAGLMLFKTAAAGRIRKGQA
                     LRPPGALTEPQFLDRCIRCQECVKICATTGACLQPALTEAGIEGLWSPVSVPREGYCE
                     YSCNLCGQVCPTGAIQPLTLAQKQKSRMGTAYFDKTRCIPWYRQEDCLVCEEHCPLPD
                     KAIKFDIREARGPDGSMRTVKFPYVVEDLCIGCGICENKCPVVGAPGIFLTAAREIRL
                     AEPGKSGLKGT"
     gene            5119..6108
                     /locus_tag="PKM23_08420"
     CDS             5119..6108
                     /locus_tag="PKM23_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91538.1"
                     /translation="MFSDLVRSEALEDGSLRITPLGSLEDISLLPAVLAVFEQEFRRK
                     HDHFVLDLSGLPALPAGWVALIFELTARARRRGGDLCVTNLQRKAQGDLLNFQPETYL
                     CLDEMDFFLPPAASFAGSAASERSDAAIQVGAAVWPEREAAAVPEPAADPALPRQAEP
                     PPALPSGSITIPSRVDALYRACDFVLDFAARQGFGESDLSRIKISVYEASLNAIEHAY
                     HSDATQKVSVEVAADSERMIIQIVDHGDGFEVKTNEDFDVTAAAAARRTGGMGLHIIR
                     RSMDRVTYHRDPKHGNRLIMEKRLPRNASPGPLREGDAEETPWPEPSGTGEMP"
     gene            6105..6623
                     /locus_tag="PKM23_08425"
     CDS             6105..6623
                     /locus_tag="PKM23_08425"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNY91539.1"
                     /translation="MSHIIRLNRRILLPLILFLLLITAIGGGLLCLRQSLPARPAEEE
                     RSEAVAEEPEEPDLAAVATSAGVVHLKRALFCLDIHKRRPVIIKSAFSRRVDYIYCYT
                     VITAPPEGVNILHRWILEGQPVFERRLRVQGKNCRVWSCRHLMNKEPGSGRVEIVVEE
                     GNMLGSSVFTLI"
     gene            6639..6872
                     /locus_tag="PKM23_08430"
     CDS             6639..6872
                     /locus_tag="PKM23_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91540.1"
                     /translation="MSAEQLRDEIRRFQRRKKWFALPLVLLGLIMLVTPGPGMAILLL
                     GFMLLFPRDGHRVLNWIRARWRRLRGSSSTPRP"
     gene            6996..7676
                     /locus_tag="PKM23_08435"
     CDS             6996..7676
                     /locus_tag="PKM23_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNY91541.1"
                     /translation="MYFGMSDILLLAPALILTLYAQFKIKSAYNKWSQVSSTSGLTGA
                     QVAKSLLQRNSILDVEVGRVEGNLTDHYDPIHKTLNLSDGVYATNSIAAIGVAAHETG
                     HAIQHNVAYSPLKLRQTMYPLANFGSMLAMPLFIIGLMVSSLKFLTTLGIAFFAASVF
                     FTLVTLPVEFNASKRALAQLTSSGMIKGEEVDGARKVLNAAALTYVAAAAMAVLQLIR
                     LLMIRNDH"
     gene            7695..>8369
                     /locus_tag="PKM23_08440"
     CDS             7695..>8369
                     /locus_tag="PKM23_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNY91542.1"
                     /translation="MNGKKRSVLLTAGIFIGIMIGLVISSNFDWVMHSIASDRAESAV
                     ALGSSEPVSQELMGLQSLSKAFTEVAKIASPAVVTINSSTVVKQSVRHPFMDDPFFRQ
                     FFDVPDQKRVMRGLGSGVIVNPDGYILTNNHVIKDADEITVMIDKVEHQAKVIGKDPA
                     SDIAVIKIDKKGLPTLNLGNSDRLEVGEWVMAIGNPFSDVLEKTVTAGIVSAKGRSLG
                     AGLGEGS"
BASE COUNT         1577 a         2714 c         2434 g         1644 t
ORIGIN      
        1 accagttggg attgctattt gaataaaaaa cggggggtac cctgtcaatt ttgccgcttg
       61 acatttttgc tttttttgcg taactttatg ttcattattt agccgctttc aagatcaaat
      121 aaaggagact caccatggct atcagaatgg gtatcaacgg attcgggcgc attggtcgcc
      181 tggtttttcg tgctgccgtt cagcgcaagg atatagagat cgttcaggtc aatgatatca
      241 ccgatgcacc gaccctcgct catctgctca agtatgactc tgttcacggg gttctcaaga
      301 acgacatccg cgccgaaggc gagtatatcg ttttcgacgg caagaaggtc aaggtctccg
      361 cggagaagga tcccgccaac ctgccctggg gcaaagagaa cattgatatc gtggtcgagt
      421 cgaccggcgt cttccgcaaa cgcgccgagg tggccaagca tctggagggt ggcgccaaga
      481 aggtgatcct gaccgttccg gccaaggatg agatcgatgc caccatcgtc atcggcgtca
      541 atgacgaagc cctgaagccc gagcacaagg tcgtctccaa tgcctcctgt accaccaact
      601 gtctggcgcc ggtcgccaag gttctgcacg acgccttcgg gatcaagcgc ggtttcatga
      661 ccacggtgca cgcttatacc aatgaccagc gcatcctcga cctgccgcac aaggatctgc
      721 gccgcgcccg cgccgccgcc gagtcgatca tccccaccac caccggcgcc gccaaggccg
      781 tcggcaaggt gattcccgaa ttgaagggca agctggatgg tttctcgctg cgcgtgccgg
      841 tttccgacgg ctcggtcgtc gatctggtcg ccgaactcaa caaggatgtc accatcgagc
      901 aggtcaacgc cgctatcaaa gccgcagcca acggcaagct caagggcatc ctcgagtaca
      961 ccgaggatcc cattgtctcc atcgacatcg tcggcaaccc cgcctcctcg atcttcgacg
     1021 ccctctcgac gatggtgctc gagggcacga gcaatttcgt caaggtcgtc tcctggtacg
     1081 acaatgagtg gggctactcc tgccgcgtcg tcgacctcgt tgaaaaaatg ggcaagatgt
     1141 aataaaactg ttggcttttc gacaattttt atgtaacttt ttggcgccgg cggggaaatt
     1201 cctttcccgc cggctgccgg acaggtgtga tacaatgcgc aaacggatta tcgccggcaa
     1261 ctggaaaatg aacaagagcg tcaccgaggc cgctgagctg gcccgggctg tcgtcgaagg
     1321 catgaagacg gcgacggaga cagaggtggt gctctgcccg cctttcactg acttgacggc
     1381 cgtcgccgct gtgatccagg ggacgccggt cgggctgggc gcgcagaacc tgcactggga
     1441 agaaaagggc gcctacaccg gcgaggtgtc ggcggccatg ctgctctcgg cgggatgcac
     1501 ccacgtcatc atcggccatt cggaacgccg ccaatacttc ggcgagaccg atgaatccgt
     1561 caaccgcaag gtcaagacgg ccctcaaggc gggactgatc ccgatcgtct gcgtcggcga
     1621 gaccctggcc gagcgccagg cggatgagac cgccgcggtg gtcgagcgtc aggtccgcgc
     1681 cgccctcgag gggctgaccg atgcgcaggt tgcggggctg gtcatcgcct acgagccggt
     1741 ctgggcgatc ggcaccgggg tggttgccac cacggagcag gcccaggagg ttcacgccct
     1801 catccgcgcc ctggtgggca aactctgcag tgaaagggtt gccgctgcgc tgcgcattca
     1861 gtacgggggc agcatgaaac cggacaatgc cggccagctg ctggcgcaga aggacatcga
     1921 cggcggcctg atcggagggg ccagtctgga tgcccgctcg tttctcgaca ttgtctatgc
     1981 atagctgata tttactggag ttcgcatgta taccgtattg atcattcttt ttgttctggt
     2041 ctgcatattg ctgacctttg tgatactgat ccagtcgagt aaaggcggcg gactggcggg
     2101 ctcgttcggc ggcatggata ccatgggtac actgttcggc ggaggccgag gcagcgcccc
     2161 ctttctgcaa aagctgacca tgatcctggc tgcggtcttt cttgccatcg cactggttat
     2221 gggcttcatg acccgcggcg gggtgagcac ccagtccctg gtcgagcagg agcgcgatcg
     2281 cctcaattcc tctcccgcgc gcacgcttcc gcagatcccc aatcagccgg cgcagacgcc
     2341 gggcaagtaa gagttcacat cgcgagaccc tgcattacaa aagccgaagt ggtggaattg
     2401 gcagacacgc tatcttgagg gggtagtgcc cacaaggcgt gcgggttcaa atcccgcctt
     2461 cggcaccagc tttcgccaag cgaatgcagg aaatcggatg cacgacagcc cacctggaaa
     2521 ggtgggctgt tttcacatgg ggtgaacccc ggcaacggag agaaaggcgg tatgatggca
     2581 gactataccc gtcgtgattt cattcgcatc ggcgccggcg ccggtgcagc cctggccgcc
     2641 ctgccgatgc ttccggaagg gctcctggct gttcccaatc ccgatctggc ggtggcgcgc
     2701 aacggcgccc ccggcctcct ggtgcgcaag gccgtggagg cgatcggcgg catggggcgc
     2761 tttgtcaaaa aagggcagac tgtcctgctc aaacccaaca tgggttggga ccgcctgcct
     2821 gaacaggccg ccaacaccaa tcccgaggcg gtcgccgagg tcgtcaggat gtgcctcgag
     2881 gcgggcgcct cccgggtgcg ggtcctcgac cgcacctgca atgagccccg gcgctgctac
     2941 atgcgcagcg gcatcgaaaa ggcggccaag gcggccggag cagaggtccg tcatatcgtc
     3001 gacagccgct tccaggatct cgccatctcc ggaggggaac tgctcacctc ctggccggtc
     3061 taccgcgatg ccctcgagtc ggacgtgatc atcaacatgc cgatcgccaa gcaccacaac
     3121 atctccggcg tcaccctcag tttcaagaac ctgatgggtc tgctcggcgg tgaccgcggc
     3181 gccctgcata accatttcat gaccaagatc gtcgacatca acatggcgat ccggccctcc
     3241 ctgaccatca tcgacgcctg gcgcatcatg ctgcgcaacg gccccaccgg cggcagcctc
     3301 gccgatgtgg ctgagaaaca gctcttcatc gcctccgcag accgggtggc tgccgatgcc
     3361 tgggccatgg ggctgttcaa tatcgatccc aacaccgtcg agtatctgcg catcgcagcc
     3421 aagcggggcc tcggccagct ggatctcaag cgggtgaaaa tccaggagat caacctcggc
     3481 gtttgatggc tgtccagcgg cgtccagaca ggccgcacag accgcaacct tacagcaatg
     3541 gagtggatag ggccatgatg ataaccctgc gccggatctc ccagctgctc ttcctgagcc
     3601 tctttgtcct gctcttcttt caggcgcgtt tcccctacga ggcctggctg cccgccgacc
     3661 ttttcctgcg ggccagccct ctggtggcct tgacggtgat gattgccagc cgcgccttta
     3721 ttgcttccac catcctcgcc ctgatcgtgc tcgccctcac catccctctc gggcgttttt
     3781 tctgcggctg ggtctgccct ctcggcacca tcatcgacgg cagcgaccac ctcatccggc
     3841 gcaagcgcag gccggcgcgg cagcgcgatc ccctcacctg gcgctcctgg aagtttttcc
     3901 tcctcgccgc gctgctggtg gccgccctct tctccaccca gttcatctgg tttttcgatc
     3961 ccatcgccct gctgacccgc accttcacca ctgtgctcca tccagccttc agctttctgc
     4021 ttggcggact tttcaatgcc gccttcggac tccatctttt cgaggaccag gtctactccc
     4081 tttatgatgc cgcccaggcc ggcggcctta tgcccgtcca gcagcccagg ctctatgaaa
     4141 atatggccat tgccctcatc ttcgccgcca tcctcggcct cggcatggtc tcgcgccgct
     4201 tctggtgccg caatctttgc cccctcggag ccctgctcgg catcttcagc aaataccgcc
     4261 tgaccaggcg ccatgtgagc gcagcctgca ccaaatgcag catctgccag cgcgaatgca
     4321 agatgaacgc catcgaggac gattacagca tcaacaacac ggtggagtgc atcgagtgtg
     4381 ccgagtgcgt gtcggtctgc aagccccggg cggtctccta caccatcggc cgcagccgcg
     4441 gtgagaaccg gatcgatttg agccgcaggc gcctgctcca ggcctcggcg gcgggggcgg
     4501 ctggtttgat gctcttcaag accgcagcgg ccggccgcat ccgcaagggc caggcccttc
     4561 gtcccccggg cgccctcacc gagccgcagt tcctcgaccg ctgcatccgc tgccaggagt
     4621 gcgtcaagat ctgcgccacc actggagcct gcctgcagcc agccctcact gaagccggca
     4681 tcgaagggct gtggagcccg gtctcggtcc cgcgcgaggg ctattgcgaa tacagctgca
     4741 acctttgcgg ccaggtctgc ccgaccgggg ctatccagcc cctgaccctt gcgcagaagc
     4801 agaagagccg catgggcacc gcctatttcg ataaaacccg ctgcatcccc tggtatcgcc
     4861 aggaggactg cctggtctgc gaggagcact gccccctccc ggacaaggcg ataaaattcg
     4921 atatccgcga ggcgcgcggc cccgacggat cgatgcggac ggtcaagttc ccctatgtgg
     4981 tcgaggacct ctgcatcggc tgcggcatct gtgagaacaa gtgccccgtc gtcggcgcgc
     5041 cgggcatctt tttgaccgcc gcgcgcgaga tccggctggc ggagccgggc aaatcgggac
     5101 tcaaggggac ctagaactat gtttagcgat ctggtgcgct ccgaagccct tgaagacggt
     5161 tccctgcgga tcaccccgct ggggagcctc gaggatatct cgcttcttcc cgccgtgctg
     5221 gcggtctttg agcaggaatt ccgcaggaaa catgatcatt ttgtgctcga tctcagcgga
     5281 ctccccgccc tgccggcggg ctgggtcgcc ctgatcttcg agctgaccgc gcgcgcccgg
     5341 cgccggggcg gagacctttg cgtcaccaac ctgcagcgca aggcccaggg agacctgctc
     5401 aatttccagc ccgagaccta cctctgtctg gatgagatgg atttttttct cccgccagcc
     5461 gcctctttcg ccggcagcgc agcctccgaa aggagtgacg cggcgattca ggtcggggcg
     5521 gccgtctggc cggagagaga agctgcggcc gtgcccgagc ccgcggccga tcccgccttg
     5581 ccccggcagg cggaacctcc gcctgccctc ccttcgggca gcatcaccat ccccagccgg
     5641 gtggacgccc tctaccgcgc ctgcgatttc gtcctcgatt tcgcagcccg acagggtttc
     5701 ggcgagtccg atctctccag gatcaagatc tctgtctatg aggcgagcct caatgccatc
     5761 gaacacgcct atcactccga tgcgacccaa aaggtcagtg tggaggtggc tgccgacagc
     5821 gagagaatga tcatccagat cgtcgaccac ggcgatggct ttgaggtcaa gaccaatgag
     5881 gatttcgatg tcaccgctgc ggccgccgca cggcggaccg gcggcatggg gctccacatc
     5941 atccgccgct ccatggaccg ggtgacctac caccgcgacc ccaagcacgg gaaccgcctg
     6001 atcatggaaa agcgtctgcc ccgcaacgct tcacccggcc ccctccggga aggcgatgcg
     6061 gaagaaacac cgtggcccga gccctccggc acgggcgaaa tgccatgagc cacatcatcc
     6121 gcctcaaccg gcgcattttg ctgccgttga tcctcttcct gctgctcatc actgccattg
     6181 gaggcggcct cctctgcctt cgccagtctc tgccggcccg cccggccgag gaggagagga
     6241 gtgaggcggt cgcggaggag cccgaggagc cggatctggc cgccgtcgcc acatcagccg
     6301 gggtcgttca tctcaaacgg gccctcttct gcctcgatat ccacaaacgc aggccggtga
     6361 tcatcaaatc ggccttcagc cgccgcgtcg attacatcta ctgctatacc gtgatcaccg
     6421 ctccgcccga gggagtgaat atccttcacc gctggattct ggagggacag ccggtcttcg
     6481 aacggcgcct gcgggtgcag ggcaagaact gccgggtgtg gagctgccgc cacctgatga
     6541 acaaggagcc aggcagcggc cgggtcgaga tcgttgtgga ggaggggaat atgctcggct
     6601 cgtctgtgtt tacattgata taaaagagaa gtgtcattat gagcgctgaa caactgcgag
     6661 atgaaatacg ccgcttccag cgacggaaaa aatggttcgc ccttcccctt gtgctgctgg
     6721 gtttgatcat gctcgtcacc cccggccccg gcatggcgat ccttcttctc ggcttcatgc
     6781 tgctcttccc ccgcgacggt caccgcgtcc tcaactggat ccgcgcgcgc tggcggcgcc
     6841 tgcgcggctc ttcctcgacc ccccgcccgt gagggacggg aactccacat cagcgcccgg
     6901 gggattccgg ctcacaaaat gaattgaaac tttttggaga ctgccgagtc taatagttga
     6961 ttcgttttac aacccccttc caaggaggag aaatcatgta ttttggaatg agtgatatcc
     7021 tgctgctggc ccccgccctg atcctgaccc tctacgccca gttcaagatc aagagcgcct
     7081 acaacaagtg gagccaggtc tcttctactt ccggtctgac cggtgcccag gtggccaaaa
     7141 gcctgctgca gcgcaacagc atcctcgatg tcgaggtcgg ccgcgtggag ggaaatctga
     7201 ccgatcatta cgatcccatc cacaagaccc tcaatctttc cgacggcgtc tatgccacca
     7261 attccatcgc ggccatcggc gtggccgccc atgaaaccgg ccacgccatc cagcacaatg
     7321 tggcttacag ccccctcaag ctgcgccaga ccatgtatcc cctggccaat ttcggttcca
     7381 tgctggccat gccccttttc atcattgggc tgatggtttc atccctgaaa ttcctcacca
     7441 ccctcggcat cgcctttttc gccgcctcgg tctttttcac cctcgtcacc ctgccggtgg
     7501 agttcaacgc ctccaagcgt gctctcgcac agctgaccag cagcggcatg atcaagggcg
     7561 aagaggtcga cggcgcgcgc aaggtgctca atgccgctgc cctgacctat gtggcggccg
     7621 cggccatggc ggttcttcag ctgatcagac tgctgatgat tcgcaacgat cattgatatt
     7681 cggaaaggaa ctatatgaac ggcaagaaac gctcggtcct gctcacggcc ggtatcttta
     7741 tcggcatcat gattggattg gtaatttcga gtaattttga ctgggtgatg cacagcatcg
     7801 cttcagatcg tgctgaaagc gccgtcgccc tcggttccag tgagcccgtc tcgcaggaac
     7861 tgatggggct gcagagcctc agcaaggcct tcaccgaggt cgcgaaaatc gccagtccag
     7921 cggtggtgac catcaacagc agcacggtgg tcaagcagag cgtccgccat cccttcatgg
     7981 acgacccctt ctttcgtcag ttttttgatg tccccgacca gaagcgggtg atgcgcggcc
     8041 ttggcagcgg agtcatcgtc aatcccgatg gctatatcct caccaacaac catgtcatca
     8101 aggatgcgga tgagatcacc gtcatgatcg acaaggtgga gcatcaggcc aaggtgatcg
     8161 gcaaggaccc ggcgagcgat attgctgtca ttaaaattga caagaagggg ctgcccaccc
     8221 tcaatctggg caactcggac cggctcgagg tcggggagtg ggtgatggcc atcggcaatc
     8281 ccttctccga cgtcctcgaa aagaccgtca ccgccggcat cgtcagcgcc aagggccgct
     8341 ccctcggcgc cggcctcggc gagggctcg
//
LOCUS       DAOPWU010000139         8367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8506_length_8367_cov_4.866097, whole genome shotgun sequence.
ACCESSION   DAOPWU010000139 DAOPWU010000000
VERSION     DAOPWU010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8506_length_8367_cov_4.866097"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PKM23_08445"
     CDS             complement(<1..797)
                     /locus_tag="PKM23_08445"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY91543.1"
                     /translation="MKIKRIHRRVLTLLIPCALLAGVTGAFGPLYGQSKTGTTIGQFL
                     LIEPSARSTAMGNAGVASTEELLSAYYNPGAIGSFKGYGVQFTHSLWLADITYDYVAA
                     AVKMGAVGNLYLSATALNSGEIAVRTVDQPLGTGEKYSVNDFAFGLGYGRQLTDRFTA
                     GVQVNFMQETIWHSSLSTFTFNVGTIYTLSGSGIRIGASIANFGLPGRYTGRDMRIVY
                     DKDPGKYGDNSNLPGQMYVEEYPLPVMFRAGIDWPLKIGHRNELHLAV"
     gene            complement(812..4099)
                     /locus_tag="PKM23_08450"
     CDS             complement(812..4099)
                     /locus_tag="PKM23_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91544.1"
                     /translation="MRWKIIVTAALISASAPAQQLQPVVPPEQRGSIDAERIGYHDAA
                     NIRTMFYNYGMVGGYPDDPGGVDLSVWHSMEVPKGSGMNYSDGTTPFILAKVKDGRGR
                     DIYIMETGYRERQGTSPTRNKVMRFEPRPGYFQADPSINKGRSPAISSDPRTWPSFWP
                     DKLNDPDDPGWVDENEDPELDDPSQAAWNGYFGKKPAADQESYTVMDDDFYDAFDFYP
                     DSRDRTRRGLGLRIEVRGFQWANPQAGNVIFWHYDITNEGTTDYNDNIIFGMYMDSGV
                     GGSALSCDGIYESDDDNAYFDKSFGLNLVYTWDKYGHGRNLLSNCGTTGYLGYAYLET
                     PGNRFNGIDDDEDGIIDERRDGGPGEQITSTDAIMAWLQAHYDIDRFTRAIGTIASRP
                     AWRASTWWTGDEDMDWVAELNDTGADGVFGTNDTGEGDGIPTLGEPNFDRTDLQESDQ
                     IGLTGFQMNRIVAGQGNPNKEVENIVFFDDGKEWPRRLFERFTNPNVGARFDSAVFLN
                     YNIGFLFASGPFILKAGQTERFSLALSYGADLYELRSTVKTVQMIYNANYQFAVPPPM
                     PKLTAETGDGWVRLSWDDAAERGIDPVSLENDFEGYRIYRSTDPEFRDPKLITTGRGT
                     GPMGNGVPIKQFDMINGRRGFSSQVVEGVAYYLGEDTGLAHSWTDTTVTNGQEYYYAV
                     TAFDHGSDSLGYYPSENAIAVSRTPRGGTILPKNVVSVRPNPRTFGFTQASASEVTQL
                     QGRGTGDIRIDVVNSNLVPDGHQFVLSFHSPSPDCVRAVNYDLMDSTTRKIIFRNGYD
                     MDGQGIGQVGLGLLPVVNTAKTVVVDSMASGFTPESQTNLHLKATYQYQQPINRRRPG
                     FPHDIRVSFYDTFRDTSLLAIGLPARPAKFLVHAVDEQGGEMRLKFRFRDLDRDGTPN
                     QSGEFIDIATFVPEKPATPQITWRLDVDTTGQAARGPIIPAKGGDTCWMRLIYPLEAG
                     DAFLFSTAGQKTDPVSARAQSKQDPYVVPNPYVGSASFEPERFAVSGRGERRIEFRAL
                     PKSCTIRIFSVRGELVQTLEHDGSDEGYIAWDLRTKDNLDVAPGLYIFHVDSELGSKI
                     GKFAIIK"
     gene            complement(4119..6788)
                     /locus_tag="PKM23_08455"
     CDS             complement(4119..6788)
                     /locus_tag="PKM23_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91545.1"
                     /translation="MRGNDLRVRRAHAAGALQRGMWMMVLIWIAAATAFAGTTGKLAG
                     RVTDRAKQPLPGANIILVGTQLGAVSDMEGYYTILNIPPGRYQVRYAFIGYKTMTVSQ
                     ILVTTDHTTTQDVQLEQSVIEGETVQVVAQRPIVATNLTSSVATVTSDAIAIMPVQDL
                     QEVVDLQAGVVDGHFRGGRTGEVQYQINGVSVNNSYDNSSTLRIDRSLLQEVQVISGT
                     FDAEYGQAMSGVVNVVLKSGTDQFAVSGEAYTSDYLYTSGDRRNAPDKFRPLSIQNYQ
                     ASLSGPLPLLPKTYFIFSGRRYANDGYVYGERRFLPTDRANFENKIYTPGGDNKEFPL
                     DTSREWSGLAKLTNRSLKNLTLEYQAIANQIAGKRYNYGMRFNPDGRAEQQTFSIVHG
                     IDLNHTLSPKTFYNISLRQNYFNYTDYAFEDFYDARYDSAGPSMGDSNYEDGASIQGV
                     ELGRFKQQTNTLVLKGALTSQVSRSHQLKFGIELQKSELKFGSPGYLVNTGGTLLVRH
                     IDEPPDYPGIAVYNPVAMVAYGQDQIEWRDLVVRAGARLEYFDARSTLPSDLANPANT
                     IAGAPLSVAKDTEAKISLSPRLGISYPVTTSASLFFAYGHFYQMPALGQIFSNSDYAI
                     LDDLQAGGISYGVLGNPDIKPERTVQYEFGYKQAIGSLLGLDVSIFYKDIRDLLGVEF
                     VSTYAAAEYARLTNIDFGNVLGYTIALDQRRIGMVSTMVDYTWQRAMGNASDPRETAT
                     RASAGEDPRPRQIPLSWDQRNTLNATVTVESPNQFAFSTIVRYGSGQPYTPALSAGFG
                     GGLETNSGRKPTGLRVDLRAEKYFKFSGLRASLFARVINLLDARFVNGFVFDDTGSPD
                     YSLNPVGQRATLANPGRYYAPRRVEIGFTFGSL"
     gene            complement(6895..>8367)
                     /locus_tag="PKM23_08460"
     CDS             complement(6895..>8367)
                     /locus_tag="PKM23_08460"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91546.1"
                     /translation="PAGPYEYFYGWVAESWADPNTANVGAAPGFFGWAAADRTVWDAV
                     TVVNGITNDDATPDNGYTTEFVFDLAPRGYDVTKPGGEIVEFNVSIYDCDWFWPINED
                     KFSSNRVWWQGPWSNNSAYAIAQLHVDPSVTVNSGALPVIGPDLIIPNGVDFADPTVD
                     GSFDDAAWAKAPGLDLRFGDDALRASYPGIGPWRSGQWQPEIDGKRAPVLDPADATLK
                     WFFKGHMLYIAADVRDQSVSGLESYDMWDGLRIIINDRAARDGESVLVRRNLTMRIGP
                     TGPVVMDFLETLFNEGKAWVGFELKPGSTINDVNDIDVGYHIEMGIDLTALGYPADLG
                     DGVLFISACLFDGDTFPNTADNYGQRVWFMRESDWPAGPAWALLDPNTKLTGVKIDPA
                     SQLPKEFALIGNYPNPFNPTTTIQFAMPEEAAVTLQVYDLLGRLVSRQVLGRQSAGMQ
                     EVSFDATRIGSGVYFYRLEMVSAKAKKTLRTSVGKMLVIK"
BASE COUNT         1512 a         2285 c         2927 g         1643 t
ORIGIN      
        1 accgcaaggt gcagctcgtt gcggtggccg attttcaggg gccagtcgat gccggcgcgg
       61 aacatcaccg gcagggggta ctcctcaaca tacatctggc ccggcagatt gctgttatcg
      121 ccgtacttgc ctggatcctt gtcgtaaacg atgcgcatgt cgcggcccgt gtatctgccg
      181 ggcaggccga aattggcgat actggcgccg atgcggatgc cggagcccga gagggtgtag
      241 atagtaccca cattgaaggt gaaggtggag aggctgctgt gccagatggt ctcctgcatg
      301 aaattgacct ggacaccggc ggtgaagcgg tcggtgagct ggcggccgta gccgaggccg
      361 aaggcgaaat cattgaccga gtatttctcg cccgtgccga ggggctgatc gacggtgcgg
      421 acggcgatct ctcccgagtt gagggcggtg gcgctgaggt agaggttgcc gacggcgccc
      481 attttgaccg cggcggcgac gtaatcatag gtgatatccg ccagccagag gctgtgggtg
      541 aactggacgc cgtagccctt gaagctgccg atggcgccgg gattataata tgcggagagg
      601 agttcctcgg ttgaggcgac tcccgcgttg cccatggctg tgctgcgggc gctgggctcg
      661 atgaggagaa actggccgat ggttgtgccg gtcttgctct ggccatagag gggcccgaag
      721 gcgcccgtca ccccggcgag cagggcgcag gggatgagca gggtcagaac gcggcgatgg
      781 attctcttga tcttcatact ccacctgtcc cttatttgat gatggcaaat ttgccgatct
      841 tgctgcccag ttcggagtcg acatggaaga tgtagagtcc gggggcgaca tcgagattgt
      901 ctttggtgcg caggtcccag gcgatatagc cctcgtcgga tccgtcatgc tcgagggtct
      961 gtaccagctc cccgcggacg gaaaagatgc ggatggtgca gctcttcggc agggcgcgga
     1021 attcgatgcg acgttcgccg cggcccgaaa ctgcaaagcg ctcgggttcg aagctggccg
     1081 aaccgacata gggattcgga accacatagg gatcctgttt ggactgggcc cgggcgctga
     1141 caggatcagt cttctggccc gccgtcgaga agaggaaggc gtcgccggcc tcgaggggat
     1201 agatcagcct catccagcag gtgtcacccc cctttgccgg gatgatcgga ccgcgggcgg
     1261 cctggccggt ggtgtcgaca tcgaggcgcc aggtgatctg gggtgtcgcc ggtttttcgg
     1321 gcacgaaggt ggcgatatcg atgaactcgc cggactgatt gggggtgccg tcgcggtcga
     1381 gatcacggaa gcggaatttc aggcgcattt cgcccccctg ctcatcgaca gcgtggacga
     1441 ggaacttggc cggccgcgcg ggcaggccga tcgccagcag ggaggtgtcc cggaaggtgt
     1501 cgtaaaagct gacgcggata tcgtggggaa agccgggacg gcgccggttg atcggctgct
     1561 gatactgata ggtggccttg agatgcaggt tggtctggct ctcgggggtg aagccgctgg
     1621 ccatgctgtc aacgacgacg gttttggcgg tgtttaccac cggcagcagg ccgagcccga
     1681 cctggccgat accctggccg tccatgtcgt agccgttgcg gaagatgatc ttgcgcgttg
     1741 tgctgtccat gaggtcataa ttaacggcgc gcacgcagtc gggcgagggc gagtggaagc
     1801 tgagcacaaa ttggtggccg tcgggcacca gattggagtt gaccacatcg atgcgaatat
     1861 cgccggttcc tcgaccctgg agctgggtca cctccgaggc tgacgcctgg gtaaagccga
     1921 aggtgcgcgg attgggacgg accgaaacga cgttcttggg caggatggtg ccgccgcgcg
     1981 gggtgcgcga gaccgcgatg gcgttctccg agggatagta gccgagagaa tcgctgccgt
     2041 gatcgaaggc ggtgacggcg taataatact cctggccgtt ggtcactgtg gtgtcggtcc
     2101 agctgtgggc caggccggta tcctcgccga ggtagtaggc gactccctca acgacctggg
     2161 aggagaagcc gcggcggccg ttgatcatat cgaactgctt gatcggcacg ccgttgccca
     2221 tcgggccggt gccgcggccg gtggtgatga gtttggggtc gcggaactcg ggatctgtgg
     2281 agcggtagat gcggtagccc tcgaaatcgt tctcgaggct gaccggatcg atgccgcgtt
     2341 cggcggcatc atcccaggag aggcgcaccc agccgtcgcc ggtttcagcg gtcagttttg
     2401 gcatcggggg cggtacggcg aactgatagt tggcgttgta gatcatctgc acggtcttga
     2461 ccgtggagcg cagctcgtag aggtcggcgc cgtaggagag agcgaggctg aagcgttcgg
     2521 tctgaccggc cttgaggata aaggggccgg aggcgaagag aaagccgatg ttgtagttga
     2581 gaaaaacggc cgaatcgaag cgcgctccca cattgggatt ggtgaagcgc tcgaagaggc
     2641 ggcgcggcca ctccttgccg tcatcgaaga agacgatgtt ttcgacctcc ttgttggggt
     2701 tgccctggcc ggcgacgatg cggttcatct ggaagccggt gaggccgatc tggtccgatt
     2761 cctggaggtc ggtgcgatca aagttgggct cgccaagggt ggggatgccg tcgccctcgc
     2821 cggtgtcgtt ggtgccgaag acgccgtcgg cgccggtgtc gttgagttcg gccacccagt
     2881 ccatgtcctc gtcgccggtc caccaggttg aggcgcgcca ggccgggcgg ctggcgatgg
     2941 tgccgatggc gcgggtgaac ctgtcgatat cgtaatgggc ctgaagccag gccatgatgg
     3001 catcggtcga tgtgatctgc tcgccgggtc caccgtcgcg gcgttcgtcg ataatgccat
     3061 cctcatcgtc atcaatgccg ttgaagcggt tgcccggggt ctccagatag gcatagccga
     3121 gatagccggt ggtgccgcag ttgctgagca ggttgcggcc atggccgtat ttatcccagg
     3181 tgtagaccag gttgaggccg aaggatttgt cgaaataggc attgtcgtca tcggactcgt
     3241 agataccgtc gcaggagagg gcggaaccgc cgactccgga gtccatgtac atgccgaaaa
     3301 tgatattgtc gttatagtct gtggtgcctt cgttggtgat gtcataatgc caaaagatga
     3361 cgttgccggc ctggggattg gcccactgga agccgcgcac ctcgatgcgg aggccgaggc
     3421 cgcggcgggt gcggtcgcgg ctgtcgggat aaaagtcgaa ggcgtcgtaa aagtcgtcat
     3481 ccatcacagt ataggactcc tggtcagcgg cgggcttttt gccgaaataa ccgttccagg
     3541 cggcctggct ggggtcatcg agctcgggat cctcgttctc gtcgacccag cctggatcgt
     3601 cgggatcgtt gagcttgtcg ggccagaagc tcggccaggt gcggggatcg ctgctgatcg
     3661 ccggcgaacg ccccttgttg atcgagggat cggcctggaa atagccgggg cgcggctcga
     3721 agcgcatcac cttgttgcgg gtcggactgg tgccctggcg ctcgcggtag ccggtctcca
     3781 tgatatagat gtcccgcccc ctgccgtcct tgaccttggc gaggatgaag ggcgtggtgc
     3841 cgtcgctgta gttcatgccg ctgcccttgg gcacctccat ggaatgccag acgctgaggt
     3901 cgaccccgcc aggatcatcg gggtagccgc cgaccatgcc gtaattgtag aacatggtac
     3961 ggatgttggc ggcatcgtga tagccgatgc gctcggcgtc gatgctgccg cgctgttcag
     4021 gggggaccac cggctggagc tgctgggccg gcgcgcttgc gctgatcagg gcggcggtga
     4081 cgattatttt ccaacgcata ggatcattct ccacagtttc acaacgagcc gaaggtaaag
     4141 ccgatttcca cacggcgcgg agcatagtag cggccgggat tggcgagggt cgcgcgctgg
     4201 ccgacgggat tgagcgaata gtcggggctg ccggtgtcgt cgaagacgaa accgttgacg
     4261 aagcgggcgt cgagcagatt gatcacccgg gcgaagaggc tggcgcgcag gccgctgaat
     4321 ttgaaatact tctcggcgcg caaatcgacc cgcagcccgg tcggcttgcg cccggaattg
     4381 gtctcgaggc cgccgccgaa gccggcgctg agagcggggg tgtagggctg gccgctgccg
     4441 tagcggacga tggtgctgaa ggcgaactgg ttggggctct cgacggtcac cgttgcattg
     4501 agggtgttgc gctggtccca gctcagggga atctgccttg gccgcggatc ctcgcctgcc
     4561 gaggcgcggg tggcggtctc gcgcgggtcg ctggcgttgc ccatcgcccg ctgccaggta
     4621 tagtcgacca tcgtgctgac catgccgatg cggcgctggt cgagggcgat ggtgtagccg
     4681 agcacattgc cgaaatcgat gttggtcagc cgggcatatt cggccgcggc gtaggtcgag
     4741 acgaattcga cgccgagcag gtcgcggata tccttgtaaa agatgctgac atcgaggccg
     4801 aggaggctgc cgatcgcctg cttgtagccg aactcgtact gtaccgtgcg ctcgggcttg
     4861 atgtccggat tgccgagcac cccgtagctg attcctcccg cctgcaggtc atcgaggatg
     4921 gcataatccg aattggaaaa gatctgcccc agggccggca tctggtagaa atggccatag
     4981 gcaaaaaaga gcgaggcgct ggtggtgaca ggataggaga tgcccaggcg tggtgaaagc
     5041 gagatcttgg cctcggtatc cttcgccacc gaaagggggg cgccggcaat ggtgttggcc
     5101 ggattggcca ggtcgctggg cagggtggaa cgggcgtcga aatattcgag gcgggcgccg
     5161 gcgcggacga ccagatcccg ccattcgatc tggtcctggc cgtaagcgac catcgcaacc
     5221 ggattataga cggcgatgcc gggatagtcg gggggctcat cgatgtggcg caccagcagg
     5281 gtgccgccgg tgttgaccag atagccgggc gagccgaact tgagttccga tttctgcagc
     5341 tcgatgccga atttgagctg gtggctgcgg ctgacctggc tggtcagggc gcccttcagg
     5401 accagggtgt tggtttgctg cttgaagcgg cccagctcca ccccctgaat gctcgcaccg
     5461 tcctcgtaat tgctgtctcc catcgaggga ccggcagagt catagcgcgc gtcgtagaaa
     5521 tcttcaaaag catagtcggt atagttaaag tagttctgac gcaggctgat attgtaaaag
     5581 gtttttggcg agagggtgtg gttgagatcg atgccgtgga cgatggagaa ggtctgctgc
     5641 tcggcgcggc cgtcgggatt gaagcgcata ccgtagttgt agcgcttgcc ggcgatctgg
     5701 ttggcgatcg cctggtactc cagggtgaga ttcttcaggg agcggttggt cagcttggcc
     5761 aggccggacc attcccgtga ggtgtcgagg gggaactcct tgttgtcacc gcccggggta
     5821 tagatcttgt tctcgaagtt ggcgcggtca gtgggcagaa agcggcgctc gccatagaca
     5881 taaccgtcat tggcataacg gcggccgctg aagatgaaat aggtcttggg cagcaggggc
     5941 agcggaccgc tcagactggc ctggtaattc tggatcgaga gggggcggaa tttgtccggc
     6001 gcgttgcgcc ggtcgccgct ggtgtaaaga tagtcgctgg tataggcctc gccgctgacg
     6061 gcgaactggt cggtgccgct cttgagcacg acattaacca cgccgctcat cgcctggccg
     6121 tactcggcat cgaaggtgcc gctgatgacc tggacctcct ggagcaggct gcggtcgatg
     6181 cgcagggtcg aggagttgtc gtagctgttg ttgacgctga caccgttgat ctggtactgc
     6241 acctcgccgg tgcggccgcc gcggaagtgg ccgtccacca cgccggcttg aagatcgacg
     6301 acttcctgca aatcctgcac cggcatgatg gcaatggcgt cactggtcac ggtggcgacc
     6361 gagctggtca ggttggtcgc gacgatcggc cgctgggcca ccacctggac ggtttcgccc
     6421 tcgatcacac tctgttcgag ctgcacatcc tgggtggtgg tgtgatccgt ggtaaccagg
     6481 atctggctga ccgtcatggt tttgtagccg ataaaggcat agcggacctg atagcgtccg
     6541 ggggggatat tgaggatggt ataatagccc tccatatcgc tgacagcccc caactgcgtc
     6601 ccgacgagga tgatattggc tccaggcagg ggctgtttgg ctctgtcggt gacacgcccg
     6661 gccagtttgc cggtcgtgcc ggcaaaggcg gtggccgccg cgatccagat gagcaccatc
     6721 atccacatgc cgcgctgaag ggcgccggcc gcatgcgcgc ggcgcaccct gagatcatta
     6781 ccgcgcatag ccttcctttc gtgaatgtgt gaccaacctc gatgatggaa aacagaagag
     6841 agggccggga ggccccggcc ccctctgttc aggctgtaca acggatggag aggactactt
     6901 gatcaccagc attttgccca cgctggtgcg gagggtcttt ttcgctttgg cgctgaccat
     6961 ctcgaggcgg tagaagtaga cgcccgagcc gatgcgggtg gcatcgaagc ttacctcctg
     7021 catgcccgcg ctctggcgcc cgagcacctg gcggctcacc agacggccga ggagatcgta
     7081 gacctgcagg gtcaccgcgg cctcttccgg catggcgaac tggatggtcg tggtcgggtt
     7141 gaagggattg ggatagttgc cgatcagtgc gaactccttc ggcagctggc tggctggatc
     7201 gatcttgacg ccggtgagtt tggtgttcgg atcgagcagc gcccaggccg gaccggcggg
     7261 ccagtcggat tcgcgcatga accagacgcg ctggccatag ttgtcagccg tgttggggaa
     7321 ggtatcgcca tcaaagagac acgcgctgat gaagaggaca ccgtcaccga ggtcggcggg
     7381 atagcccagg gcagtcaggt caatccccat ctcgatatgg taaccgacgt cgatgtcgtt
     7441 gacgtcattg atggtcgagc cgggcttgag ttcaaagcct acccaggctt tgccctcgtt
     7501 gaacagggtc tcaagaaaat ccataaccac cggaccggtg gggccgatgc gcatggtgag
     7561 attgcggcgg accagaacac tctcgccgtc acgtgccgca cggtcgttga tgataatccg
     7621 cagaccgtcc cacatgtcat aggattccag accggagacc gactggtcac ggacatcggc
     7681 ggcgatatag agcatatgac ccttgaagaa ccatttcagg gtagcgtcag ccgggtcgag
     7741 gactggagcc cgtttgccgt cgatctcggg ctgccactgg ccggagcgcc aggggccgat
     7801 gccgggatag gaggcgcgca gggcatcatc gccgaaacgc aagtcgaggc cgggggcctt
     7861 ggcccaggct gcatcatcaa agctgccatc cacagtcgga tctgcgaaat cgacgccgtt
     7921 ggggatgatc agatcagggc cgataaccgg aagcgcgccg ctgttgacgg tgaccgatgg
     7981 atcgacatga agctgcgcaa tggcataagc ggagttgttg ctccagggtc cctgccacca
     8041 gacgcggttg ctggagaact tgtcctcgtt gatcggccag aaccagtcgc agtcatagat
     8101 cgagacgttg aattcgacga tctcgccgcc gggcttggtg acgtcatacc cgcgcggggc
     8161 cagatcgaag acgaattcgg tggtgtagcc gttgtcgggc gtggcatcgt cgttggtgat
     8221 gccgttgacg acggtgacgg cgtcccagac ggtgcggtct gccgcggccc agccgaagaa
     8281 gccgggggcg gcgccgacgt tggcggtgtt gggatcggcc cagctctcag cgacccagcc
     8341 gtagaaatat tcataggggc cggccgg
//
LOCUS       DAOPWU010000140         8313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8581_length_8313_cov_5.399370, whole genome shotgun sequence.
ACCESSION   DAOPWU010000140 DAOPWU010000000
VERSION     DAOPWU010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8581_length_8313_cov_5.399370"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKM23_08465"
     CDS             complement(<1..500)
                     /locus_tag="PKM23_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY91547.1"
                     /translation="MCRKSRTALLPRYLEALQQEIAHYRRHPFFAGKTLTSIYWGGGT
                     PSLIPPEQIHALLGEIRSGWEITPSAEITLEANPGALDCAHLAGYRQAGINRLSLGIQ
                     SFHDRELALLTRIHSAAGAMEALTAARAVGFTNLSLDLIFGLPGQYLSDYKASLSQAL
                     AFGPEHL"
     assembly_gap    506..515
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(590..1300)
                     /gene="lepB"
                     /locus_tag="PKM23_08470"
     CDS             complement(590..1300)
                     /gene="lepB"
                     /locus_tag="PKM23_08470"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNY91548.1"
                     /translation="MSSKGRQQFKEYTEAFAVALIAALILRMLVIQAFRIPTGSMKDT
                     LLIGDFLLVNKFVYGAATPDRIPWTDIKLPSLQMPAVHKPKRGEIIVFKYPLDEKLDY
                     IKRCIGVPGDTLEMRQGVVYVNGAPEGEITFLERSFDESEGRYINYYRVKTANAKEYT
                     IRRYEGFAYDFGPVVVPEKHLFMMGDNRDNSQDSRYWGFLPMKNIRGQALVIYFSLER
                     NASFFRHVRWSRIFSIIR"
     gene            complement(1332..2948)
                     /locus_tag="PKM23_08475"
     CDS             complement(1332..2948)
                     /locus_tag="PKM23_08475"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91549.1"
                     /translation="MLGLLLQNRLRALINAVRRGGKKGRRRILGYIGLIILPVLVLIN
                     IYAMMEAMMAAPELGLPAILLLQRNILLAIFIMLLFSGLALVLHIFFLSRDLPLLMSA
                     PVPLATIFQFKFIETTSANSTLFFGIALPVLISAGIAVKAPLLFWILLLPLSLLFLAI
                     PTGLATLLAMGLVSVMPARRAKSISALFLSLISIAIWLGFQMLRPERIRPGAAGLPGQ
                     NYLELGGRLATWFPSDWLVNALMHTTTGRTAAAGLNILLLLGAGALLYSGAAALLGRA
                     LHRDTFSGIESGSRSRRVTRQTVRIAPAGKSGLFATLVRRDFKLIFRDTQQLIQILLF
                     TTMMVLLPFLNRTNMAAESGELAHYYPYFFLFIFSTLISSTISTRMIPMERTAFGLFK
                     LAPVRLRTVWLAKVTVSFLFTLTSALFAAAVVAFFHHTSAGVLLRVLLLLVILNIGAT
                     SCGGLFGARYANFSWDHPKRMLNAGSGFFLSLILMVFVGIVGGIVALSLFILSTPDPG
                     IAASAALTLFLLAFGTASAEKKLDKIEWTY"
     gene            complement(2953..3711)
                     /locus_tag="PKM23_08480"
     CDS             complement(2953..3711)
                     /locus_tag="PKM23_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY91550.1"
                     /translation="MLQVEQLHKAFGDRIAVEELSLQIENELFVFLGPNGAGKTTTIK
                     MMTGLLHPDRGRVLIDGIDLATDPIGAKRLFGLVQESPVLYEKLSAREFVHFMARLYQ
                     VPDEDAARRAGQLFDIFEISDRADELIEEFSHGMKQKVALAGALVHEPRVLFLDEPTV
                     GLDPKAARNLKDLLRGMVERGTTVFMSTHILEVAERMCDRAGIIHQGRLIALGTMDEL
                     RKNSATEKASLEEIFLQLTASEVGADVNRFLEEQ"
     gene            complement(3793..5586)
                     /gene="lepA"
                     /locus_tag="PKM23_08485"
     CDS             complement(3793..5586)
                     /gene="lepA"
                     /locus_tag="PKM23_08485"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNY91551.1"
                     /translation="MTKNPIRNFCIVAHIDHGKSTLADRLLEATGTLSSYEMKDQVLD
                     SMDLERERGITIKMHPVTMEYRRPNGETYTLNLIDTPGHVDFTYEVSRSLAACEGAIL
                     VVDAAQGVEAQTVSNLYLAIENDLEIIPVINKIDLPNIDLDAIKHQIIDLLGCKESEI
                     ILASAKVGTGISEILEAVVERVPPPDPAVDKPARALIFDSVFNAYRGAVAYVRVIEGS
                     IRPGQKIKFFSTGRVFEAQEVGILRMKQIPQDLLHAGEVGYVIAGAKDVKDTKVGDTI
                     TTEENPAPEPLPGYREVLPMVFSGLFPSIAENYEELRAALEKLKLNDSALWYEPETSL
                     ALGFGFRCGFLGLLHMEIVQERLEREYDLDLVTTVPNVEYHVFTNKGTLLVMDNPANL
                     PSPNDIDHVEEPYIKASIVTPSEYIGAVMSLAMERRGTYINTEYVDKSRVNMHYEFPL
                     SEVIFDFYDKLKSVTRGYASFDYEFSGFREGPVVKLDILLNGESVDALSTIVHRDKAY
                     EWGRRVCEKLSELIPRQQFEVAIQAAIGSKVIARETVRAVRKNVTAKCYGGDITRKRK
                     LLEKQKEGKKRMKQLGRVEVPQEAFLAILKS"
     gene            5783..6904
                     /gene="bshA"
                     /locus_tag="PKM23_08490"
     CDS             5783..6904
                     /gene="bshA"
                     /locus_tag="PKM23_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNY91552.1"
                     /translation="MKIAMVCYPTHGGSGVVATELGMCLALRGHEVHFISYSTPFRLR
                     GFHENVFYHEVDVSAYPLFKYPPYDLSLAAQIMEVAGEHTLDIVHAHYAIPHAISAYL
                     ARQMMRGRGPKVVTTLHGTDITLVGKDASFREAVKFGIEESDGVTAVSRYLAEQTRAT
                     FGIDKEIRVIHNFVDTARFSPHVENCIRKRFAPGGEAILIHASNFRPVKRLTDTIEIL
                     ARVRRHLPAKLLLVGEGPDRQESHALAERLGLLDHVVYLGTQDAVERLLGCADLFLLP
                     SSEESFGLAALEAMSCGTPVIATRIGGLPEVIEDGVDGFLFPVGDVDAMAAAAVELLR
                     EPERMKAMRAAARAKAEACFRIETLVPEYESYYKQVLGG"
     gene            7078..>7886
                     /locus_tag="PKM23_08495"
     CDS             7078..>7886
                     /locus_tag="PKM23_08495"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNY91553.1"
                     /translation="MTAPNNSQRWQNFLAHMRSNSWRGYVLIGVILYLVLINQLIRVR
                     PDHVFIALAVLALTLGRGRMKYFVMDWAPWIVFWICYDMMRGVVDNWRGAINVVWPYM
                     TELKIFGPLFGGVIPCFWFQDLQQALQGNWLKGLLDASGGLFYTLHFGVPIIVGWVFW
                     HTTDDRKSFYRFVWTMTLLNFGALATFFIYPAAPPWYVYSYGFGQPAVSSHWGLGAGA
                     LLNVDKMIGMNFFQTLWGGFNPNHFAAIPSLHGAYPVAFALFMYKKFRRYPL"
     assembly_gap    7887..7896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7897..8088
                     /locus_tag="PKM23_08500"
     CDS             <7897..8088
                     /locus_tag="PKM23_08500"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91554.1"
                     /translation="LLLALYPAGVWFSAVYLNQHYIIDLFIGAGYLLIAYVITDRLLI
                     PHVFSRFISWAPLHKKANA"
     gene            8240..8312
                     /locus_tag="PKM23_08505"
     tRNA            8240..8312
                     /locus_tag="PKM23_08505"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8273..8275,aa:Phe,seq:gaa)
BASE COUNT         1589 a         2342 c         2687 g         1675 t
ORIGIN      
        1 agatgttcag gcccgaaggc gagggcctgg ctgagcgagg ctttatagtc ggaaaggtac
       61 tgaccgggca atccgaaaat gaggtcgagg ctgagattgg tgaatcccac tgcgcgcgcg
      121 gccgtgaggg cttccatcgc ccccgcggcg gagtgaatcc gggtgagcag ggcgagttcg
      181 cgatcgtgaa aggactggat gcccagcgag agccggttga tccccgcctg gcggtagccg
      241 gcgagatggg cgcagtcgag ggcgccggga ttggcctcaa gggtgatctc agccgaggga
      301 gtgatctccc agccggagcg gatctccccg agcagggcat ggatttgttc cggcggaatc
      361 agcgaggggg tgccgccgcc ccagtagatg ctggtgaggg tcttgccagc gaagaaggga
      421 tggcggcggt agtgggcgat ctcctgctgc agcgcctcga gatagcgcgg cagcagggcg
      481 gtgcgacttt ttctgcacaa cccgcnnnnn nnnnngagaa aaagtcgcaa tagatgcact
      541 tgtgctggca aaaggggacg tggatataga gcgcaagggc atccatggcc taacggatga
      601 tgctgaagat gcgcgaccag cgcacatgac ggaaaaacga ggcgttgcgt tccagggaga
      661 aatagatgac cagagcctgg ccccggatgt tcttcatcgg cagaaagccc cagtagcggc
      721 tgtcctggct gttgtcgcga ttgtcgccca tcatgaagag atgcttttcg ggcaccacca
      781 ccgggccgaa atcgtaggcg aatccctcat aacggcggat ggtgtattcc ttggcgttgg
      841 cggtcttgac ccggtaatag ttgatatagc gcccctccga ttcgtcaaag ctgcgctcga
      901 ggaaggtgat ctcaccttcg ggagcgccgt tgacgtagac caccccctgg cgcatctcga
      961 gggtgtcgcc gggcacaccg atgcagcgtt tgatatagtc aagcttttcg tcgagggggt
     1021 atttgaagac gatgatctcg ccgcgtttgg gtttatggac ggccggcatt tgcagggagg
     1081 gcagcttgat gtcggtccag ggaatgcggt ccggggtcgc tgcgccataa acgaacttgt
     1141 tgacgaggag aaaatccccg atcagcaggg tgtctttcat cgagccggtc gggatgcgga
     1201 aggcctgaat gaccagcatg cgcaggatga gggcggcgat gagagccacg gcgaatgctt
     1261 cggtatactc cttgaactgc tggcgtcctt tactgctcat gcaaggtcct ttcgtagtgg
     1321 cgagggtgta atcaataggt ccattcaatt ttatctaact tcttttccgc gctggcggtt
     1381 ccaaatgcga gcaggaacag ggtgagcgcg gcggaggcgg cgatgcccgg atccggcgtc
     1441 gagagtataa agaggctgag ggcgacgatg cccccgacga taccgacgaa aaccatgaga
     1501 atgagggaga gaaaaaagcc cgagccggcg ttgagcatcc gtttcggatg gtcccagctg
     1561 aaattggcgt agcgggcgcc gaagagcccg ccgcagctgg tggcgccgat gttcaggatg
     1621 accagcagca ggaggaccct gagcagcacc ccggcgctgg tatggtgaaa aaaggccacg
     1681 acggcggcgg cgaagagggc gctggtcagg gtgaagagaa agctgaccgt caccttggcc
     1741 agccagacag tgcgcaagcg gaccggggcg agcttgaaga gcccaaaggc ggtgcgctcc
     1801 atcgggatca tgcgcgtcga gatggtgctg ctgatgaggg tgctgaagat gaagagaaag
     1861 aaatagggat aataatgcgc aagctctccg ctctccgcgg ccatgttggt gcggttgaga
     1921 aagggcagca ggaccatcat cgtcgtgaag agcaggatct ggatgagctg ctgggtgtcg
     1981 cggaagatga gcttgaaatc ccggcggacg agggtggcga agaggccgct tttccccgcc
     2041 ggcgcgatgc gcacggtctg ccgggttacg cgccggctgc gggatccgct ctcgattccg
     2101 ctgaaggtat cccggtgcag ggcgcggccg agcagggcgg cggcgccgct gtagagcagg
     2161 gccccggcgc ccagcagaag gagaatattc aagccggcgg cggctgtgcg tcctgtggtg
     2221 gtgtgcatca gggcgttgac cagccagtcc gagggaaacc acgtcgccag ccgcccgccc
     2281 agctcgagat agttctgccc cggcagcccg gccgctccgg gccggatcct ctccggccgc
     2341 agcatctgaa agcccagcca gatcgcgatc gagatcaggc tgagaaagag ggcggagatg
     2401 cttttggccc ggcgcgccgg catgacgctg accagcccca tcgccagcag ggtggccaag
     2461 ccggtcggga tggcgaggaa gagcaggctg aggggcagca gcaggatcca gaagagcagg
     2521 ggcgccttga cggcgatgcc ggcggagatc aggacgggca gggcgatgcc gaaaaagagg
     2581 gtggaattgg ccgaggtggt ctcgatgaat ttgaactgga aaatggtggc caggggcact
     2641 ggggcgctca taagcagggg gagatcccgt gagaggaaaa aaatgtgcag caccagggcc
     2701 agaccgctga aaaggagcat gatgaagatg gcgagaagga tgttgcgctg gagcaggagg
     2761 atggcgggaa ggcccagctc gggggcggcc atcatcgcct ccatcatcgc atagatgttg
     2821 atcagcacca gtaccggcag gatgatcagg ccgatgtacc ccagtatgcg gcggcgccct
     2881 tttttgccgc cgcggcgcac ggcgttgatc agggcgcgaa gccggttttg cagcagcagt
     2941 cccagcatgc ctctactgtt cctccaggaa ccggttgacg tcggcgccga cctccgaagc
     3001 ggtcagctgc agaaagatct cctcaagcga ggctttttcg gtcgccgaat ttttgcgcag
     3061 ttcatccatg gtgcccagcg cgatgagccg gccctgatgg atgatgccgg cgcggtcgca
     3121 catccgctcc gccacttcga ggatatgggt ggacatgaag acggtggtgc ctcgttcgac
     3181 catgccgcgc agcagatcct tgagattgcg cgcggccttg ggatcgagcc ccacagtcgg
     3241 ctcatcgaga aagagcaccc gcggctcatg gaccagggcc ccggccaggg ccaccttctg
     3301 cttcatgccg tgggagaatt cttcgatgag ctcatcggcg cggtcgctga tctcgaaaat
     3361 gtcgaagagc tgcccggcgc ggcgcgcggc atcctcatcg ggcacctgat agagccgcgc
     3421 catgaaatgg acgaattcgc gcgcgctcag cttttcatag agcaccggcg actcctgcac
     3481 cagcccgaag agccgtttgg ctccgatggg gtcggtggcc agatcgatgc cgtcgatgag
     3541 cacccgaccc cggtcggggt ggagaagccc ggtcatcatc ttgatggtgg tggttttgcc
     3601 ggcgccgttg ggaccgagaa agacgaagag ctcgttctcg atctggagcg aaagctcttc
     3661 cactgcgatc ctgtcgccga aagccttgtg cagctgttca acctggagca ttgcagtctt
     3721 ttttcccccg agattatacc gggctccggc cggcaggccg ggttgtgtgg agacaagacg
     3781 ggcgtggcgc cgtcacgatt tgaggatggc gagaaacgct tcctggggca cttccacccg
     3841 gcccaactgc ttcatacgct tcttgccctc tttttgtttc tcgagcagct tgcgcttgcg
     3901 ggtgatgtcg ccgccgtagc atttggcggt gacattcttg cgcaccgcgc gcacggtctc
     3961 gcgggcgatg accttgctgc cgatggcggc ctgaatggcg acctcgaact gctggcgcgg
     4021 tatcagctcg ctcagctttt cgcagacgcg gcgcccccat tcgtaggcct tgtcgcgatg
     4081 gacgatggtc gacagggcgt cgaccgattc gccgttgagc aggatgtcga gcttgacgac
     4141 cggaccttcg cgaaaaccgg agaattcata atcgaacgag gcgtagccgc gggtgaccga
     4201 tttgagtttg tcgtaaaaat caaagatgac ctcgctgagc gggaactcgt agtgcatatt
     4261 gacccggctc ttgtcaacgt actcggtatt gatgtaggtg ccgcgcctct ccatcgccag
     4321 cgacatcacc gcgccgatgt actcggaggg ggtgacgatg ctcgccttga tgtagggctc
     4381 ctcgacgtga tcgatgtcgt tgggcgaggg caggttggcc ggattgtcca tcaccagcag
     4441 ggtccccttg ttggtgaaga catgatactc cacattgggg accgtggtca ccaggtcgag
     4501 gtcatactcg cgttcgaggc gctcctggac gatttccatg tggagcaggc cgagaaagcc
     4561 gcagcggaag ccgaagccga gggcgaggga ggtctcgggt tcgtaccaga gtgccgaatc
     4621 gttgagcttg agcttttcca gggcggcgcg cagctcctcg taattctcgg cgatggaggg
     4681 aaagaggccg gagaagacca tgggcagcac ctcacggtag ccgggcaggg gctccggggc
     4741 cggattttcc tcggtggtga tggtgtcgcc gaccttggtg tccttgacat ccttggctcc
     4801 ggcgatgaca tagccgacct cgccggcatg gaggagatcc tgcgggatct gcttcatgcg
     4861 caggatgccg acctcctggg cctcgaacac ccggccggtg gaaaagaatt tgatcttttg
     4921 ccccggccgg atcgagccct cgatcacgcg cacgtaggcc acggccccgc gataggcatt
     4981 gaagaccgag tcgaagatca gggcgcgcgc cggcttgtcc accgccggat cgggcggcgg
     5041 gacgcgctcc accaccgcct cgaggatttc actgatgccg gtgccgacct tggcgctggc
     5101 caggatgatc tctgattcct tgcagccgag caggtcgata atctgatgct tgatggcgtc
     5161 gagatcgatg ttggggagat cgatcttgtt gatgaccggt atgatctcca ggtcgttttc
     5221 aatggccagg tagagattgc tgacggtctg ggcttccacc ccctgggcgg cgtccaccac
     5281 caggatggcc ccctcgcagg cggccagact gcgcgagacc tcataggtga aatcgacatg
     5341 gcccggagta tcgatgaggt tgagggtata ggtttcgccg ttggggcggc ggtactccat
     5401 cgtgaccgga tgcattttga tggtgatgcc gcgctcgcgc tcgaggtcca tgctgtccag
     5461 gacctgatcc ttcatctcat agctggagag ggtgccggtg gcttcgagca ggcggtcggc
     5521 cagggttgat ttgccgtggt cgatatgggc gacgatgcag aaatttcgga tcggattttt
     5581 ggtcatatac ccgctggcaa gttgtgattg gtcaatagga catttaaatt aaacaaatta
     5641 tgcaaataaa tcaataaaaa agcggacgcg ggccggcccc ggagcctgct gctgcggagg
     5701 cgggaatctt attccctctg tcagcgttct ctaagcggtg gggtgatgta atgaccagaa
     5761 caaatgaaat aacggagtgt gcatgaaaat agcgatggta tgctatccga cccacggcgg
     5821 cagcggcgtg gtggcgacgg aactgggcat gtgtctggcg ctgcggggcc atgaggtcca
     5881 tttcatcagt tacagtaccc ccttccgcct gcgcggtttt cacgagaacg tcttttatca
     5941 cgaggtggat gtctccgcct atcccctttt caaatacccc ccctatgacc tgagtctggc
     6001 cgcgcagatc atggaggtgg ctggtgagca taccctcgac atcgttcatg cccactatgc
     6061 catcccccac gccatcagcg cctacctggc gcggcagatg atgcgcgggc gcggaccgaa
     6121 ggtggtcacc accctgcacg gcaccgacat caccctggtc ggcaaggatg cctccttccg
     6181 ggaggcggtg aaattcggca tcgaagagtc cgacggagtg accgcggtct cccgctactt
     6241 ggccgagcag acgcgcgcga cttttggcat cgacaaggag atccgcgtta ttcacaattt
     6301 tgtcgacacc gcccgcttct caccccacgt ggagaactgc atccgcaagc gcttcgcgcc
     6361 ggggggagag gcgatcctca tccacgcctc caatttccgc ccggtcaagc ggttgaccga
     6421 taccattgag atcctggcac gggtgcgccg ccacctgccc gccaagctgc tgctggtagg
     6481 cgagggaccg gaccggcagg agtcgcatgc cctggccgaa cggctcggcc tcctcgatca
     6541 tgtcgtctat ctcggcaccc aggatgccgt cgaacggctg ctcggctgcg ccgatctctt
     6601 cctcctgccc agcagcgagg agagctttgg actggccgcc ctcgaggcga tgagctgcgg
     6661 caccccggtg atcgccaccc gcatcggcgg cctgccggag gttatcgaag atggcgtgga
     6721 tggttttctc ttcccggtcg gggatgtcga tgccatggcg gcggcagcgg tggagctgct
     6781 gcgcgagccg gaacggatga aggcgatgcg ggcggcggcg cgcgccaagg ccgaggcctg
     6841 cttccgcatc gagaccctgg tccctgagta cgaaagctat tacaagcagg tgttgggggg
     6901 ctgattcccc cggcgccggc cgagctggtt taccctcatc cggcggaaga aattggcgtt
     6961 ttcagtccgc actattcccc acttgtcgcc gagaaatcct ttttcctgaa atatttttta
     7021 tgtatattct tccgccttga aaccggcccg gacaagtcag atgagaacac gagagatatg
     7081 accgccccca acaactcgca gcgatggcaa aattttctcg cgcacatgcg ctccaacagc
     7141 tggcgcggat atgtgttgat cggggtgatt ctctacctgg tgctgatcaa ccagctcatc
     7201 cgggtgcggc cggatcatgt cttcatcgcc ctggcggttc tggccctcac cctcggccgt
     7261 gggcgcatga agtattttgt catggactgg gcgccgtgga tcgttttctg gatctgctac
     7321 gacatgatgc gcggcgtggt cgacaactgg cggggcgcca ttaatgtggt ctggccctat
     7381 atgaccgagc tgaaaatttt cggccccctg ttcggcgggg tgattccctg cttctggttc
     7441 caggacctgc agcaggccct gcagggcaac tggctcaagg gtctgctcga cgccagcggc
     7501 ggactttttt acaccctcca tttcggcgtg ccgatcatcg tcggctgggt cttctggcac
     7561 accaccgatg accgcaaatc cttttaccgc tttgtctgga ccatgaccct gctcaatttc
     7621 ggcgccctcg ccactttttt tatctatccg gcggcaccgc cgtggtacgt ttacagttac
     7681 ggcttcggcc agcctgccgt ctcgagccat tggggcctgg gcgctggtgc gctgctgaat
     7741 gtggacaaga tgatcggcat gaattttttc cagaccctgt gggggggatt caatcccaac
     7801 cactttgccg ccattccctc attgcacggt gcttatccgg tggcctttgc gctttttatg
     7861 tacaaaaagt tcaggcgcta tcccctnnnn nnnnnnctgc tgctggccct ctatccggcc
     7921 ggagtctggt tttcggcggt ctatctcaac cagcattaca tcatcgatct gttcatcggc
     7981 gccgggtacc tgctcatcgc ctatgtcatc accgaccggc tgctcatccc ccatgtgttc
     8041 tcgcgcttca tcagctgggc gccgctgcat aaaaaggcga acgcctgaac cggtcttttc
     8101 ttgatgccct ccaaaacaga gtcccacccg gatgtaatca tgagaaaatc agttggcagg
     8161 cagatttcgg ggggttccct ggttgttctt ttcccttgac tttttgccat aaaatgaata
     8221 atttgtatgc acgtttggcg gcgctttagc tcagccggta gagcaacgga ctgaaaatcc
     8281 gtgtgtcccc agttcgattc tgggaggcgc cac
//
LOCUS       DAOPWU010000141         8308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8588_length_8308_cov_4.531807, whole genome shotgun sequence.
ACCESSION   DAOPWU010000141 DAOPWU010000000
VERSION     DAOPWU010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8588_length_8308_cov_4.531807"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PKM23_08510"
     CDS             <1..676
                     /locus_tag="PKM23_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91555.1"
                     /translation="ILIGLAIIPLAATMTEPERKKASGQNPLKNILHIAAESMKNPDI
                     RPFILLLGLIGSVSVISLWAYFLFYQVVGIPIAFFGVLFAAFQFSGALGARFSAAAAA
                     KAGTLRFLMVPLLIAPILLLVGLLRSPWLLPLIMFHPFIWNLTIPVLLQQINLRSTSQ
                     VRATVLSLANMGVSFGYVLIGPVFGRLSDRLPLGYCFLFLSLIYLIFTLVLLSTIRRH
                     WHLRHG"
     gene            complement(757..1821)
                     /locus_tag="PKM23_08515"
     CDS             complement(757..1821)
                     /locus_tag="PKM23_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91556.1"
                     /translation="MKKTAPFILLALLFAFTAIQAQVTYESVTTSDGFMGMGASVSTD
                     KTFIQGDARREESQIKFTGGIMKHMSPKGSEIEITRLDKEVIWKMNDKDKKYTQMTFA
                     EFKKMMEEGLLDQPMMGKPDQKPENKPEGEPEYEWQTPVVKARDLGEKKELNGFSCKH
                     YLVTVTTIGKQVSTGKLDTLLLSADMWNSLNAASAMKAANDFEMRLAKALGLDKMAEA
                     MGPMMAQYGEQFKKLAEEMKKVEGYSVLTDMTLAMTTHSMPKPSKEELEAAQAEPEPE
                     PADVTDVKGAFGKMLGKKIKDAAKAKAEKDKAEKAKGNPNQKVLFHTITELKSITGGT
                     LAADLFEVPASYKLQENKKK"
     gene            2129..3295
                     /locus_tag="PKM23_08520"
     CDS             2129..3295
                     /locus_tag="PKM23_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNY91557.1"
                     /translation="MNFVFLSPHFPPNYYQFCVHLHHLGVNVLGIADEPYENLHPELK
                     AVLGEYYHVSDMHNYDELLRGLGWFTHRHGKLDRLDSQNEYWLETEARLRSDFNIFGL
                     NNANIAHIKRKSLMKEMYARAGVPYAEGAVTASLAEARRLVKRIGYPVVAKPDIGVGA
                     SRTYKIHNDAELDRFFAQKPPVDYLIEEFIQGTIVTFDGLTDQQGEPVFYTSHIYSNG
                     VMETVNEAGDVYYYSLREIPADLEEAGRRVLRAYDLRERFFHLEFFRTGKEGRIVALE
                     VNMRPPGGLTTDMFNYACDIDIYHEWAHVVTHNRFTANYKRKYHCCYAGRKFGKRYAH
                     SHEGILRRFPHQLVHHQTITGLFSAAMGDYGYLFRSPDVEEIEAIAAFILEPQV"
     gene            3315..4049
                     /locus_tag="PKM23_08525"
     CDS             3315..4049
                     /locus_tag="PKM23_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNY91558.1"
                     /translation="MHIEHHTWWSSRLGQNMSVNVYGHAGKPVLVFPCQGGTHREYED
                     FGMIEAVSGFIDSGKIKLFTVDSVDDQSWNNRRVHPAQRVARHKEYESYINEEVLPLI
                     YYHHQAEGKLMVTGCSMGGYHAANYFFRRPDLSDVMISISGLFQLDYFIGDYMDDQVY
                     FHTPLAFLPGLSDPWYIDQYRQSRIIVGVGQGEWEEPMVVDALKLKNILEEKGVPAWI
                     DLWGHDVNHDWPWWRQMMPHFLSTLF"
     gene            complement(4072..5022)
                     /locus_tag="PKM23_08530"
     CDS             complement(4072..5022)
                     /locus_tag="PKM23_08530"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNY91559.1"
                     /translation="MEHKSNLKVAVVGAGQVGATFAYALLIRGLASEIVLIDANRALA
                     EGQAEDMSHGLPYVRPVHIFAGAYSDCRDADIIVITAGAAQKPGETRLELTRRNVDIY
                     RQIIPEITRHTTSAILIVVSNPVDVLTYAAVKLSGLPPQRVIGSGTTLDSARFRYLLS
                     QHCGVDPRNVHAMIIGEHGDSEVPVWSLANISGLRMDDYCAQCGKGCPQLRKDEIFEQ
                     VRTAAYHLIEKKGATYHAIGLATLYVIESIARDQNSVLTVSSLLQGYKGIADVAISVP
                     SILNRSGISAHIDLPLNEKEQAGLRRSAEVIQAAIRAAGL"
     regulatory      5169..5285
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5347..>7055
                     /locus_tag="PKM23_08535"
     CDS             5347..>7055
                     /locus_tag="PKM23_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91560.1"
                     /translation="MRKQCIFLAAVLALTAAVYDSAQAAPPWSLSGRVYDGQSGAPLA
                     GTQILVEGTRLGTVSGDDGRFLLRRIESAEAVLTVRLLGYETARLQVSSTGSVEPAIA
                     LKPTVLPLQSILITASRTRETEWSASTAALRQQEIQQRHTVQDIPVLLSELPSTTFYS
                     ESGNGIGYTYLNLRGFDQRRVSVLVNGVPQNDPEDHMVYWLDFPDLAANLEQIQVQRG
                     AGSLFSGTPAIGGAINLITSNFSRRRGITLTGGYGTFNTRKAGLTASSGLIDNRYAIY
                     GRLSRITSDGYRDNSWADLGSYFLGAARFDRSMTTQINLYGGPVSDHLAYYGVPAAAL
                     GDRAARRDNPIRRPEEIENFSQPHYEFIHEWRLGRTTLHNTLFYVRGEGFYDYDGSWA
                     PWSYYRITSDNGFEVPGNPDELYIPNALIHAAVENDQYGWMPRAQINHKRGIFTLGGE
                     ARLHSSLHYGTLKWGEGLPAGVTPDYRYYEYRGGKRMFSLYANEVYTAAERLRLLAEA
                     TLVHHTYRLHDEKYVGTDFSVPYLFFNPKIGAHYHFSSHWNGYLTLARTSREPRLKNL
                     YDAA"
     assembly_gap    7056..7155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7156..7565
                     /locus_tag="PKM23_08540"
     CDS             <7156..7565
                     /locus_tag="PKM23_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91561.1"
                     /translation="SGNLTWSSNTLVRYTQYGEEGALRLDGNAIAGFPGLLANARVSY
                     RSEGWELALAGRHVGSFYTSNRELPAERVAACTLADLHLGYTLERVPGLQSVAWQVTV
                     TNLFDALYAAGGEGEEFFPGATRSVYVGMNLEL"
     gene            7567..8202
                     /locus_tag="PKM23_08545"
     CDS             7567..8202
                     /locus_tag="PKM23_08545"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNY91562.1"
                     /translation="MAHWKKALVIANGPLPSRRRIAGWLDEETLIVCADGGANRARAR
                     ALIPHLVIGDLDSITPGTAAALAGVETIRVEDQENTDLEKVLDYLLAHGITAAVVLGA
                     TGRRPDHTLANFSILAKYHSRLDLLFVDSWCRMEIIDRPVQLDLAPGTTLSMLPLGRC
                     EGVTTGGLAYPLVHQVLEPGVRESISNRVSKSPVEISLTVGKLLLFIVDKK"
BASE COUNT         1504 a         2538 c         2396 g         1770 t
ORIGIN      
        1 catcctcatc ggactggcga tcattccgct ggccgcaacc atgaccgagc cggagcggaa
       61 aaaggccagc gggcaaaatc ccctgaaaaa tatcctgcac attgctgccg aatcgatgaa
      121 aaaccccgat atccggccct tcatccttct ccttggactg atcgggtccg tcagtgtcat
      181 ttcgctttgg gcatactttt tgttttatca ggttgttggc attccgatcg catttttcgg
      241 cgttctcttc gccgcctttc agttttcggg cgccctcggc gcccgatttt cagctgctgc
      301 tgcggccaaa gccggtaccc tccgttttct catggttccc ctgctgatcg cgcccattct
      361 tctgctggtt ggactgctgc gctcgccatg gctgctgccc ctcatcatgt tccatccctt
      421 catatggaat ctgaccatcc cggtgctgct gcagcagatc aacctcagaa gcacgtcgca
      481 ggtcagggcg acggtgctct ccctggccaa tatgggggtc agtttcggct atgtgctcat
      541 cggcccggtt ttcggccgcc tcagcgaccg gctgccgctg ggatactgct tcctcttcct
      601 ctcattgatc tacctgatct tcacactcgt gctcctctca accatccgca ggcactggca
      661 cctccggcat ggatagcgtg tggccgccgc cgttggcgag ggagggacaa aaaaaagccc
      721 gccaggaggc ctggcgggct tgtgtgcgca taaaggctat ttcttcttgt tttcctgcag
      781 cttgtagctc gccgggacct caaagaggtc ggcggccaga gtgccgcctg taatgctctt
      841 gagttcggtg atggtgtgga agagcacctt ctgattcgga ttccccttgg ctttctccgc
      901 cttgtccttt tccgccttgg ccttggctgc gtccttgatc tttttgccga gcatcttgcc
      961 aaaggcaccc ttgacgtcgg taacatcggc gggctcaggc tccggctccg cctgggcggc
     1021 ctcgagctcc tctttagacg gcttcggcat gctgtgcgtc gtcatggcga gggtcatatc
     1081 agtgagaacg gagtagccct cgaccttttt catctcctcg gccagctttt tgaactgctc
     1141 gccatactgg gccatcatcg gccccattgc ctccgccatt ttatccaggc cgagggcctt
     1201 ggccaggcgc atctcaaagt cgttggccgc cttcatcgca ctggcggcgt tgaggctgtt
     1261 ccacatgtcc gcagagagca ggagggtgtc gagtttgccg gtggagacct gcttgccgat
     1321 agtggtgacg gtgaccagat agtgcttgca gctgaagccg ttgagctcct tcttttcgcc
     1381 gaggtccctg gccttgacaa ccggggtctg ccattcgtat tcaggctcgc cctcaggctt
     1441 gttctccggt ttttgatccg gtttgcccat catcggctga tcgagcagcc cctcttccat
     1501 catcttttta aactcggcga aggtcatctg ggtgtacttt ttgtccttgt cgttcatctt
     1561 ccagatcacc tctttatcga ggcgggtgat ctcgatttcg ctgcccttgg ggctcatgtg
     1621 tttcatgatg ccgccggtga atttgatctg gctctcctcg cggcgggcgt cgccctggat
     1681 gaaggtcttg tccgtcgaga ccgaggcgcc catgcccata aagccgtcgc tggtggtgac
     1741 gctctcgtag gttacctgcg cctgaatggc ggtgaaggcg aagagcaggg cgagaaggat
     1801 gaagggagct gtttttttca tgatgaaacc tccggattga ttcgaggtcg gccgccggcg
     1861 gtgcggagtg cgtccgggcc gggatggccg attaattggt gtaacaaagg ggtggaggga
     1921 ggcattcccg ccaggcagcg ggaaatgcga tccatgccaa tattgctgcg tataacctct
     1981 gacatgaaaa tataccggca atccgccgat tgtgaggaat ttccggcacg gcatgctgtg
     2041 ttttttactt gtgtcatgcg ggaatgttcc ctcgccgcat caaaagcatc cggaatggat
     2101 tggaaaccgt caagcagggc cagcaaagat gaattttgtc tttctctctc cccattttcc
     2161 gcccaattat taccagtttt gcgttcatct ccaccatctt ggcgtgaacg tcctcggcat
     2221 cgctgacgag ccctacgaga atctccatcc cgagctcaag gcggtcctgg gggagtatta
     2281 tcacgtttcg gacatgcaca attatgatga gctcctgcgc ggcctgggct ggttcaccca
     2341 ccgtcacggc aagctggacc gccttgattc ccagaacgaa tactggcttg agaccgaggc
     2401 acgcctgcgc agcgatttca acatcttcgg gctcaacaac gcgaatatcg cccacatcaa
     2461 gcgcaagtcg ctgatgaagg agatgtacgc cagggccggc gtcccctatg ccgagggggc
     2521 ggtgaccgcc agcctggccg aggcgcgccg gctggtcaag cgcatcggct atccggtggt
     2581 ggccaagccc gacattgggg tgggggcctc gcggacctac aaaatccaca acgatgccga
     2641 gctggaccgc tttttcgcac agaaaccgcc ggtcgattat ctcatcgagg agttcattca
     2701 aggcaccatc gtcaccttcg acggactcac cgaccagcag ggtgagccgg ttttttacac
     2761 cagccacatc tatagcaacg gggtgatgga gacggtcaac gaggcgggtg atgtctatta
     2821 ctactccctg cgcgagattc cggcggacct cgaggaagcc gggcgccgcg tcctgcgcgc
     2881 ctacgacctg cgcgagcgct ttttccatct tgaatttttt cgcaccggca aggagggccg
     2941 catcgtcgcc ctcgaagtca acatgcgccc ccccggcggc ctgaccacgg acatgttcaa
     3001 ctatgcctgt gatatcgata tctatcacga gtgggcgcac gtggtgactc acaaccgctt
     3061 caccgccaac tataagcgca aataccactg ctgctacgcc ggccgcaaat tcggcaaacg
     3121 ctatgcccac agccatgaag ggatcctgcg ccgttttccc caccagctgg tccatcacca
     3181 gaccataacc ggccttttca gcgcggcgat gggggattat ggctaccttt tccgctcgcc
     3241 cgatgtcgaa gagatcgagg cgattgcagc ctttattctc gaaccgcagg tgtgaaggga
     3301 gaaaccagaa gaacgtgcat atagaacatc acacctggtg gagcagccgg cttggccaga
     3361 acatgtcggt caacgtctac ggccatgccg gcaaaccggt gctggtcttt ccctgccagg
     3421 gcggcacgca ccgggaatat gaggatttcg gcatgatcga ggcggtctcc ggctttattg
     3481 actccggcaa gatcaagctt ttcaccgtgg acagcgtcga cgaccagtcc tggaacaacc
     3541 ggcgggttca tcccgcccag cgcgtcgccc gccacaagga gtatgagagc tatatcaacg
     3601 aggaggtgct gcccctcatc tattatcacc atcaggcgga gggcaaactg atggtgaccg
     3661 gctgcagcat ggggggctat catgcggcca attatttttt ccgccgtccg gacctgagcg
     3721 acgtgatgat ttcgatcagc ggcctcttcc agctcgatta ttttatcggc gactatatgg
     3781 atgaccaggt ttatttccac accccgctcg ccttcctgcc cggcctcagc gatccctggt
     3841 atatcgacca gtaccggcag agccgcatca tcgtcggggt cggccagggg gagtgggagg
     3901 agccgatggt cgtggacgcc ctcaaactca agaatatcct cgaggagaag ggggtccccg
     3961 cctggatcga cctctggggg cacgatgtca accatgactg gccgtggtgg cgacagatga
     4021 tgccccattt cctctccacc ctcttctaga cctttctgcc tcaggctcga actagagacc
     4081 tgctgcacga atggccgcct ggatgacctc ggctgaacgg cgcagtccgg cctgttcctt
     4141 ttcattgagg ggcagatcga tatgagcgct gatgccgctg cggttgagaa tcgagggcac
     4201 gctgatggcc acgtccgcga tccccttgta cccctgcagc aggctcgaga ccgtgagtac
     4261 tgaattctgg tcacgcgcaa tgctttcaat gacatagagg gttgccaggc cgatggcgtg
     4321 gtaggtggct cccttttttt caatgagatg ataggccgcg gtgcgcacct gctcaaagat
     4381 ttcgtccttg cgcagctgcg ggcagccctt gccgcactgg gcacaatagt cgtccatgcg
     4441 caggccggag atattggcga ggctccagac cggcacttcg ctgtcaccgt gctcgccgat
     4501 tatcatcgca tgcacgttac gcgggtcaac cccgcagtgc tggctgagaa gataacggaa
     4561 gcgggcgctg tccagggtcg taccagagcc gatcacccgc tgcggcggca ggccggagag
     4621 cttgaccgcg gcataggtga gcacgtcgac cggattggag acgacgatga ggatggcgga
     4681 ggtggtgtgc cgcgtgatct cgggaatgat ctgccggtag atatcgacgt tgcggcgggt
     4741 caattcgagg cgggtttcgc cgggcttttg cgccgctccc gcggtaatca cgatgatatc
     4801 ggcgtcgcgg caatcgctgt aagcgccggc gaatatgtgc accggccgca cataggggag
     4861 accgtggctc atatcctcgg cctggccctc ggccagcgcc cggttggcat cgatgaggac
     4921 gatctcggag gcaaggccgc ggatgagcag ggcataggcg aaggtcgcac cgacctgccc
     4981 ggcgccaacc acagccacct tgaggttcga tttatgttcc atgacagaga ctcccattat
     5041 atttcgtata tacgaaaaca ataacctccg caaggcaagg gctattcccc ggctgtgcag
     5101 ggaaatggtt gaaataaaag cagccggtag ggtgttctga ttggtgcatt gaaacgttct
     5161 ttatggcttc ccatagttag gggtgtctcg ccaccttttt gcgagtctga gatcataccc
     5221 tgtgtccctc gggacgatga cctgatccgg gtaataccgg cgtaggcaaa cacatgcgca
     5281 aatttgcgtg ttcaagccgt ctctccgtcc cggaggcggc tttttttgtt ggagtgaggt
     5341 gaacaagtgc gaaagcaatg catatttcta gcagccgtgc tggccctgac cgctgcagtt
     5401 tacgattctg cacaggcggc tccgccgtgg tctctttctg gacgggtcta cgacggccag
     5461 agcggcgccc cactggccgg aacgcagatc ctcgtcgagg gcacccggct ggggaccgtc
     5521 agcggggacg atggacgctt cctcctgcgc cgcatcgagt ccgccgaggc ggttctgacc
     5581 gtgcgtctgc tcggctatga gaccgcgcgg ctgcaggtct cttccacggg cagcgtcgaa
     5641 ccggccatcg ccctcaagcc gacggtcctg cccctgcaga gcatcctcat caccgcctcg
     5701 cgcacgcgcg agaccgaatg gtcggcttcc accgccgctc tccgtcagca ggagattcag
     5761 cagcgccaca ccgttcagga catcccggtg ctgctttcag agctgccttc aacgaccttt
     5821 tactccgaga gcggcaacgg catcggctac acctatctca acctgcgcgg cttcgaccag
     5881 cgccgggttt cggtgctggt caacggtgtg ccccagaacg atcctgaaga ccacatggtc
     5941 tactggctcg attttcccga tctggccgcc aatcttgagc agatccaggt gcagcgcggg
     6001 gcgggcagcc tcttcagcgg caccccggcg atcggcgggg cgatcaacct gatcacctcc
     6061 aacttttccc gccgccgcgg catcaccctg accgggggat acggcacctt caacactcgc
     6121 aaggccggcc tcaccgccag ctcggggctc atcgacaacc gctatgccat ctacgggcgg
     6181 ctcagccgca tcaccagcga cggttaccgc gacaacagct gggctgatct cggcagctat
     6241 ttcctcgggg cggcccgctt cgaccgcagc atgaccaccc agatcaatct ctacggcggt
     6301 ccggtctcgg accatctcgc ctattacggg gtgccggccg cggccctggg cgaccgggcg
     6361 gcgcggcggg acaaccccat ccgccggcct gaagagatcg agaatttttc ccaaccccat
     6421 tacgagttca tccatgagtg gcgcctcggc cggaccactt tgcacaacac cctcttttac
     6481 gtgcgcggtg agggctttta cgattatgac ggctcctggg ctccatggag ctattaccgc
     6541 atcacctctg ataacggatt tgaggtgcca gggaatccag acgagctcta catccccaat
     6601 gccctcatcc acgctgctgt cgagaatgac cagtacggct ggatgccgcg ggcgcagatc
     6661 aaccacaagc gcggcatctt caccctcggc ggcgaggcgc gcttgcacag ttcactgcac
     6721 tatggcaccc tcaaatgggg tgaggggctg ccggcgggcg taactcccga ttatcgctat
     6781 tatgaatacc gcggcggcaa gcgcatgttc tccctttacg ccaacgaggt ctatacggcg
     6841 gcggaaaggc tgcgcctcct cgcggaggcg accctggtcc accacaccta ccgcctgcac
     6901 gatgaaaagt atgtcggcac cgactttagc gtgccctatt tattcttcaa tcccaagatc
     6961 ggggcccact atcacttttc ttctcactgg aacggctacc tcaccctggc gcgcaccagc
     7021 cgcgagccgc ggctgaagaa cctctacgat gcggcnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnntgagc ggcaacctca cctggagcag caacaccctg gtgcgttaca
     7201 cccaatacgg ggaagagggg gcgctgcgcc tcgacggtaa cgccatcgcg ggtttcccgg
     7261 ggctgctggc caacgcccgg gtgagctacc gcagcgaggg ctgggagctt gcgctcgccg
     7321 gccgccacgt cggctccttt tacacctcca accgggagct tcccgccgag cgggtggcgg
     7381 cctgcacgct ggccgatttg catctgggct atactctcga gagggtgccg gggctgcagt
     7441 cggtcgcgtg gcaggtgacg gtcaccaatc ttttcgatgc cctctatgcg gccggcggcg
     7501 aaggggagga attctttccc ggggcgacgc ggtcggttta tgtgggcatg aatctggagc
     7561 tttaatttgg cgcactggaa aaaggccctc gtcatcgcca acggccccct gccctcgcgg
     7621 cggaggatcg ccggatggct ggatgaggag accctcatcg tctgcgctga cggcggggcc
     7681 aaccgcgccc gcgcgcgcgc gctcatcccc catctggtca tcggcgacct ggactccatt
     7741 acgcccggca cggctgcggc cctggccggg gtggagacca tccgcgttga ggatcaggag
     7801 aataccgacc tcgagaaggt cctcgattat ctgctggcac atgggatcac cgctgccgtg
     7861 gtgctcggcg cgaccggccg ccgtcccgac cataccctgg ccaatttcag cattctggcc
     7921 aaataccact cccgtctcga tcttctcttc gtcgattcct ggtgccggat ggagatcatc
     7981 gaccggccgg tgcagctcga tctggcgccg ggcaccactc tctccatgct gcccctcggg
     8041 cgctgcgagg gggtgaccac gggggggctg gcctacccgc tcgttcatca agtccttgag
     8101 ccgggggtgc gcgagagcat cagcaaccgg gtgagcaaaa gcccggtcga gatcagcctg
     8161 acggtgggga aactgctgct ctttatcgtc gacaaaaaat agcatccgga acggagttct
     8221 gatgatcacc tttgcgccgg tcgatattgt tctgattctt ctctacttcg ccgtcatact
     8281 ctggatcggc ttccgcgtga aaattccg
//
LOCUS       DAOPWU010000142         8306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8590_length_8306_cov_4.861835, whole genome shotgun sequence.
ACCESSION   DAOPWU010000142 DAOPWU010000000
VERSION     DAOPWU010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8590_length_8306_cov_4.861835"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /locus_tag="PKM23_08550"
     CDS             complement(<1..1725)
                     /locus_tag="PKM23_08550"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNY91563.1"
                     /translation="MRFSSLLFLLLTLSVQTARPGPDELRQDPFLEEVQRRTLRYFID
                     TTPVETGLAPDRYPSPGPCSIAAAGYALTAFPLAVEHRMLTRSQAAERTVKALRFFRG
                     LAAHPQAETTTCHKGFYYHFLKIPSGEREWNCELSNMDTALLIMGALFCQGYFDRPDS
                     IEAEIRALADSLYFAADWNWFMNGRETMSMGWHPEEGFNPTSWTGYMESMILYILGLG
                     SPTHPLPDGVWEAWCSTYIWAPYHGQEFVSFGPLFGHQFSHVWIDFRAIQDAWMRARG
                     IDYFENSRRATLSQQDYAIANPGGWRDYGEHIWGFTASDGPADTEVVIDGITRRFSTY
                     SARGVSFDWINDDGTIAPYAAGSSMPFAPEICLPAIKEMRRRYGSRLWTRYGFLDAFN
                     PTYRLGKGNDRGWFNPDYLGIDQGPMLAMIENYRTGLIWRVLAQNPHLLRGLRRAGFS
                     GGWLDASPLDQFGSLEGWKSIAADGVSLRTTLEEQSAGGRALRLDFHFIAGGGYCGVQ
                     KQLPITLPENFQFTFRIRGHAPANNLEFKLLDASGDNVWWHIKRNYHWPQEWSTITIK
                     RRHIDFAWG"
     gene            1925..3961
                     /locus_tag="PKM23_08555"
     CDS             1925..3961
                     /locus_tag="PKM23_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNY91564.1"
                     /translation="MAEFTIRALIIGLIMCVVLGAANAYLGLKAGMTIAATYPAAVIG
                     MAVLRLFRNKGTMLEQNFARTVGSIGESVAAGAIFTLPAFYISGLWRPFFSTSHYLTA
                     TIILIVGGVLGIMFVALLRRVMVEDATLPFPESVAAAEIHKAGSSSGTGSKYLFGAMI
                     GGAVVKMLAEFKLFAETWEHFIAFARKTITGTAIPGEGGILISSPGISPAYMGVGYII
                     GPKLGALNFSGGLLAWGLLTPIILYFLTPSMTPDFLAGWAAYLMGLDSTLTQGSATAR
                     VSDPVFQMYAVWRYIVKPIAIGGMLMAAGFTLFKMRKSLIAGIGRSVSDVKKAASGSA
                     VEYARTEKDMKFMWVVVGILASAVVTFLVTKFIFGTSLLVALVAATVLIILAFFFAAI
                     SGYLVGIMGSSNNPISGLTLTALVVTALILVAIGVGKTNAGVAAVLGVAAIVCVGAAV
                     AGEMLQDLKAGHILGGTPWKMQIGNLIGVVIAGAVMFGVLHFLNQADIAQGIKEGYDG
                     GFGSPKLSAPQAGLMSMLSQGIVGGQMSWPLIICGMMMGLAFILMQVRSPMLVAVGMY
                     LPLETTFAIFVGGVVKGIVDMVSERRSFNSAQRARVENVGILLAAGFIAGEALTGLAF
                     APFKIAQVNLWRIFDKAPISIGLLVLLIISLILILVPIRNAGKPDEPAPPAVSM"
     gene            complement(4058..5680)
                     /locus_tag="PKM23_08560"
     CDS             complement(4058..5680)
                     /locus_tag="PKM23_08560"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNY91565.1"
                     /translation="MKSWLIILLLALTTWPAAALTEGPAGSAHGAANALALEDSAFLD
                     LLQRSAVNFFWYEANSSNGLIKDRSTPNSACSIASVGFGLTAICIGIDHGWIPADQGR
                     QRVLTTLQTFWNKPQGRDARGFIGYKGLFYHFLYMSSAVREGYTELSDIDSALLLAGI
                     LYSREYFEGADSTDVEIRALADSIYRRVDWQWMRNYNPGLTTEWTPEKGFGGGWWSGY
                     NEMMIMYILGLGSPTHPLPASTWKAFCGGYKWGVQYGQEYVNFPPLFGHQFSHCWIDY
                     RGIQDDYMRAKGIDYFENSRRATLAQQAYCIANPRGWKGYGEKVWGITPSDGPDGYEA
                     RGAPPAENDDGTIAPTAVGGSLPFAPEICLPTLHHFYDTYRSQLWGSYGFRDAFNLQR
                     NWWATDVIGIDQGPIALMIENYRTGKIWKTFMKNADVRRGLERAGFRPSGTGVETTPA
                     GQPHGFHLRQNYPNPFNSETVLEYDLPAGGSVSLSIYDLGGRLVRCLADGPRSAGLHR
                     VVLSSEGLSSGLYFCRLIFGGQSLVRKLEVIR"
     gene            complement(5691..8084)
                     /locus_tag="PKM23_08565"
                     /pseudo
     CDS             complement(5691..8084)
                     /locus_tag="PKM23_08565"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
     gene            complement(8110..>8306)
                     /locus_tag="PKM23_08570"
     CDS             complement(8110..>8306)
                     /locus_tag="PKM23_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91566.1"
                     /translation="LFMKTLALRAGYQNLWLQDSEVGLTLGTGIVFELGGALFQADYA
                     WADHGRLENTQRFTLGLALE"
BASE COUNT         1450 a         2488 c         2787 g         1581 t
ORIGIN      
        1 tccccaggcg aaatcgatgt ggcgcctttt aatcgtgatc gtgctccact cctgcggcca
       61 gtgataattg cgtttgatgt gccaccagac gttgtcgccg ctggcgtcga ggagcttgaa
      121 ttcaaggttg ttggcggggg catggccgcg gatccggaag gtgaactgga aattctccgg
      181 cagggtgatg ggcagctgtt tctgcacgcc gcagtagccg ccgccggcga tgaaatgaaa
      241 gtcgaggcgc agggcgcgcc cgcctgcgga ctgctcctcc aaagttgtgc gcagactgac
      301 cccgtcagcg gcgatgcttt tccatccctc aaggctgccg aactggtcga gaggggaggc
      361 gtcgagccag ccgccgctga aaccggcgcg gcgcagaccg cggaggagat ggggattttg
      421 ggccagcacg cgccatatca gaccggtgcg atagttctcg atcatggcca gcatcggtcc
      481 ctggtcgatg ccgagatagt caggattgaa ccagccccgg tcgttgccct tgccgaggcg
      541 gtaggtgggg ttgaaggcgt cgagaaagcc atagcgggtc cagagacggc tgccgtagcg
      601 gcggcgcatc tccttgatgg cgggcaggca gatttcgggg gcgaagggca tggagctgcc
      661 ggcggcgtat ggggcgatgg tgccgtcgtc attgatccag tcgaaggaga ccccgcgggc
      721 gctgtaggtg gagaagcggc gtgtgatccc gtcgatgacc acttcagtgt cggcggggcc
      781 gtcgctggct gtgaagcccc agatatgttc gccgtaatcg cgccagccgc cggggttggc
      841 gatggcatag tcctgctggg agagggtagc ccggcgggag ttttcgaaat aatcgatccc
      901 cctggctcgc atccaggcgt cctggatggc acggaaatcg atccagacat gggagaactg
      961 atggccgaag agggggccaa aactgacgaa ttcctggccg tgataggggg cccagatata
     1021 ggtggagcac caggcctccc agaccccgtc gggcagggga tgggtggggg aaccaagacc
     1081 gaggatgtag aggatcatcg actccatgta gccggtccag gaggtgggat tgaaaccctc
     1141 ctcgggatgc cagcccatcg acatggtttc gcgtccgttc atgaaccagt tccagtccgc
     1201 cgcgaaatag agcgagtcgg ccagggcgcg gatctccgct tcgatggagt cggggcgatc
     1261 aaaatagccc tggcagaaga gggcgcccat gatgagcagg gcggtgtcca tattcgagag
     1321 ctcgcagttc cattcgcgct caccgctcgg aatttttaaa aagtggtagt aaaatccctt
     1381 gtggcaggtg gttgtttcgg cctgaggatg ggcggcgagt ccgcggaaga agcgcagggc
     1441 cttgacggtg cgctccgccg cctggctgcg ggtgagcatg cggtgctcga cggcaagggg
     1501 aaaggcggtg agggcgtatc ccgctgcggc gatgctgcag ggcccgggtg aagggtaacg
     1561 gtccggggcg aggccggtct cgaccggtgt ggtatcgatg aaatagcgga gggtgcgccg
     1621 ctgcacctcc tcgagaaagg gatcctgccg aagttcatcc gggcccggcc gcgcggtctg
     1681 gacggagagg gtgagcagga ggaacaaaag ggagctgaac cgcatgattg cctcgagttt
     1741 ttttgattga ttatttcatg ctgaatggct actttagcac cactgatgaa acgactcggc
     1801 ccttcctgtc tatttcaggg gaagtgaaat ccccttcaat ttcaacccta cacactcacc
     1861 agcagaggag aacctcatgt ccgatcctca aaagttcgtt ccgttcgttt ctcctgaatc
     1921 aaagatggct gaatttacga tccgggcgct gatcatcggt ctgatcatgt gcgtcgtcct
     1981 cggcgcggcc aacgcctatc tcggcctgaa agccggcatg accatcgccg cgacctatcc
     2041 ggcggcggtg atcggtatgg cggtcctccg cctcttccgc aacaagggga cgatgctcga
     2101 acagaatttc gccaggaccg tgggatcgat cggcgaatcg gttgcggcgg gtgccatttt
     2161 caccctgccg gctttttaca tttccggcct ctggcgtcca tttttctcca ccagccacta
     2221 tttgaccgcg actatcatcc tcatcgtcgg cggcgtcctg ggcatcatgt tcgtcgccct
     2281 gctgcgccgg gtgatggtgg aggatgccac cctgcccttc cccgaatcgg tggcggcggc
     2341 cgagatccac aaggccggca gctcctccgg aaccggttca aagtacctct tcggcgccat
     2401 gatcgggggt gctgtggtca agatgctggc cgagttcaag ctcttcgcgg agacctggga
     2461 gcatttcatc gcctttgcgc gcaagaccat caccggcaca gccatccccg gcgagggcgg
     2521 catcctgatc agctcgcccg gcataagccc ggcctacatg ggagtcggct atatcatcgg
     2581 gcccaagctc ggcgccctca acttcagcgg cggtctgctc gcctggggcc tgttgacgcc
     2641 gatcatcctc tatttcctca ccccctccat gacccccgat tttctcgcgg gctgggcggc
     2701 ctatctgatg gggctcgatt ccaccctcac ccagggttca gcgacagcca gggtgagcga
     2761 tccggttttc cagatgtacg cggtctggcg ctacatcgtc aagccgatcg ccattggcgg
     2821 catgctgatg gcggccggtt tcaccctctt caagatgcgc aagagcctga tcgccggcat
     2881 cggccgttcg gtcagcgatg tcaaaaaggc cgcatcgggc agcgcggtgg aatatgcgcg
     2941 caccgagaag gacatgaagt tcatgtgggt ggtggttggc atcctcgcct ctgcggtcgt
     3001 gacctttctg gttaccaagt tcatcttcgg caccagtctt ctggtcgccc tcgtggccgc
     3061 cacggtgctg atcatcctcg cctttttctt cgccgccatt tcgggctatc tggtcggcat
     3121 catgggctcg agcaataacc ccatcagcgg cctgaccctg accgccctgg tggtgaccgc
     3181 cctgatcctg gtcgccatcg gcgtcggcaa gaccaatgcc ggtgtggcgg ctgtgctcgg
     3241 cgtcgctgcc atcgtctgcg tcggcgccgc cgtcgccggc gagatgctcc aggacctcaa
     3301 ggccgggcac atcctcggcg gtaccccctg gaaaatgcag atcggcaacc tcatcggcgt
     3361 ggtcatcgct ggcgctgtca tgttcggggt tctccatttt ctcaatcagg ccgacatcgc
     3421 ccagggcatc aaagagggct atgacggcgg tttcggcagc cccaaactct ccgcgcccca
     3481 ggcgggcctg atgtccatgc tctcgcaggg catcgtcggc ggccagatgt cctggcccct
     3541 gatcatctgc ggcatgatga tggggctggc cttcatcctc atgcaagtgc gcagtccgat
     3601 gctggtcgcg gtcggcatgt atctgcccct tgaaaccacc ttcgccatct tcgtcggcgg
     3661 cgtggtcaag ggcatcgtcg atatggtcag cgagaggcgc agcttcaact ccgcccagcg
     3721 ggcgcgggtc gagaatgtcg gtattcttct cgccgccggt tttatcgccg gagaggctct
     3781 caccggcctg gccttcgcac ccttcaagat cgcccaggtc aatctctggc gcatcttcga
     3841 caaggcgccg atttcgatcg gcctgctggt gctgctgatc atctcgctga ttctgatcct
     3901 ggtgccgatc cgcaacgcgg gcaagcccga tgagccggcc ccaccggcgg tttcgatgta
     3961 ggatcgcctc tgaaacagaa agacggaagc actgcttccg tctttttttt tatcccgctg
     4021 cgaggagggg cgggacggag gcggctgccg acccggatta cctgatcacc tccagcttgc
     4081 gcaccaggga ttggccgcca aagatgagac ggcagaaata gaggccgctg gacagcccct
     4141 ccgaggagag gaccaccctg tgcaggccgg cgctgcgggg gccatcggcc aggcaacgca
     4201 caagccggcc gcccagatca taaatggaga ggctgacgct gccgcctgca ggcagatcat
     4261 actcgaggac ggtctcgctg ttgaagggat tgggatagtt ctggcgcaga tggaagccgt
     4321 gcggctgccc ggccggcgtg gtttcgaccc ctgtcccgga gggccggaaa cccgcccgct
     4381 caagtccgcg ccggacatcg gcgtttttca taaaggtctt ccagatcttg ccggtgcggt
     4441 agttctcgat catcagggcg atgggcccct ggtcgatgcc gatgacgtct gtcgcccacc
     4501 agttccgctg gaggttgaag gcgtcgcgga aaccgtagga gccccagagc tggctgcgat
     4561 aggtgtcgta aaaatggtgc agggtcggca ggcagatctc cggggcaaag gggagggagc
     4621 ctcccaccgc cgtcggcgcg atggtgccgt catcgttctc ggctggcggg gcgccacggg
     4681 cttcataacc gtcggggccg tcgctggggg tgatgcccca gaccttttcg ccgtacccct
     4741 tccagcccct gggattggcg atgcagtagg cttgctgggc cagggtggcg cggcgcgaat
     4801 tttcgaaata atcgatgcct ttggcgcgca tatagtcgtc ctgaatgccg cggtagtcga
     4861 tccagcagtg cgagaactga tggccgaaga gggggggaaa gttgacatac tcctggccgt
     4921 actgcacccc ccatttgtaa ccgccgcaga aggccttcca tgtcgatgcc ggcaggggat
     4981 gggtgggcga gccgaggccg aggatgtaca tgatcatcat ctcgttgtaa cccgaccacc
     5041 agccgccgcc gaagcccttc tcgggggtcc attctgtggt cagccccggg ttgtaattgc
     5101 gcatccactg ccagtcgacg cggcgataga tcgagtcggc aagggcgcgg atctcgacat
     5161 cggttgaatc cgccccttcg aaatactcgc ggctgtagag gatgccggcc aggagcaggg
     5221 cgctgtcgat gtccgacagc tcggtgtagc cctcgcgcac ggcgctgctc atgtagagaa
     5281 agtgatagaa taatcccttg tagccgataa aacccctggc atcccgtccc tggggtttgt
     5341 tccagaaggt ttgcagggtg gtgagaaccc gctgcctccc ctggtcggcg gggatccagc
     5401 cgtggtcgat gccgatgcag atggcggtga ggccgaaacc gaccgaggcg atgctgcagg
     5461 cgctgttggg ggtgctgcgg tccttgatga ggccgttgct gctgttggcc tcgtaccaga
     5521 agaaattgac cgccgagcgc tggagcaggt cgagaaaagc cgaatcctcg agggctagag
     5581 cattcgccgc accatgcgcg gagccggccg gaccctctgt gagtgcggcc gccggccagg
     5641 ttgtgagggc caaaaggagt atgatcagcc aggatttcat caatgcttcc tcatttcatg
     5701 atgacgtgca cccggtgtgc gctgcccgcc ggaaagggcg gcagcacgca ggcgccatca
     5761 ggccgccgtt cggggaagga tacgccatcc acctcgacgg tgagcccgcc tttttccact
     5821 ccccggggat tttccactgt gatctggtag accgccccgc ggaagcggcg ctcgaccctg
     5881 aatcccttcc agtcgggggg taggcagggg tcgatgagca gcccttcatg ggtggcgcgc
     5941 agaccgagga tgccctccat ggcgacgcgg aagagccagg cggccgaacc ggtgtaccag
     6001 ctccagcccc cgcggccgaa ctgcggcgaa tccgggccgt cgacgttgcc cggggtgacg
     6061 tagggttcgc tctgatagag ctcgggctcg aggccgcgca ggacgggatt gatgcggctg
     6121 aagatggcgt aggcgcgggc gccccggccc aggagggcct cggccagcac cgcccaggtg
     6181 ccggcatggg tgtagagccc gccgttttca cgccggcccg gggcatagcg ggtgatgtag
     6241 ccgatgtcgg gatccacggc gccataggcc ggggcaaaga ggagcgggcc gtactcccgg
     6301 tcgagatgct cctcgacggc ggccatcgcc gccccggcgc gttcaggaga ggcggtgccg
     6361 ccgatgaccg cccaggtctg ggcgttgagg tagatccgcc cctgcgcgca agaggcgctg
     6421 cccagcttct cgccgctgtc cttggtggcc cgtcagtacc aggccccgtc ccaaccatgc
     6481 tcgttaacgg cagtacggag cgcctccgcc tccggggcgc agaaggccag ctcctccgcc
     6541 agcccggcgg gagcggccag ctctgcccat tcccgcagga tgcggtggag gaaatggccc
     6601 atccaaaccg actcgccctt catgccggtg ccaacggcat tgagtccgtc gttccagtca
     6661 tgggcgccga tgaggggcag gccgcgcggg ctgcgccatt ccagagcgcg cttgagggcg
     6721 cggcggcagt gctcccagag cggctggggc tgctcgtcat cgacaaaagg cagcctctcc
     6781 tccagaaggg agaaatcccc gctctccttc agatactcgg ccgtcacaaa gggcagccag
     6841 aggaggttgt cgctgacgct gctgtgcagc cctgcatcgg tgaaagggtg ccaccagtga
     6901 tagaccgatc cgtctttgaa ctgatggcgg gcgtggaggc ggatctgggc ggcggtctgt
     6961 tcgggggcga ggggcagcca gatcaggctg tcctgaagct gatctcggaa gccgtaggcc
     7021 ccgctctgct ggtaataggc ggcgcgggcc cagagccggc atgagatcgc ctggtatttt
     7081 agccaggtgt tgaggagcag atcaaaggcg ggatcggggg tctcgacgcg aaagggggcg
     7141 aggcgctcct gccatccctt cctgacccgc tccagcgcat cgcgggcggc gccagggctg
     7201 cggtattttt gcgtcagttc ataggcctga gcggacgagt cggcggcgcc gaggacgaag
     7261 actatttcct gctcctcatc gggctggagg gtgaactgga gctggaggct ggcgatgccg
     7321 tcgccccatt tgccctggct gttccggcag gagccggtct gcactgcgtg gggcgcagcc
     7381 agatcgccga gacggccgat aaagctctcc ttgtcgccct caaagcctgc gaccgatgcg
     7441 gaggcggcga aaaaggcggt ccagggccag acgacgttcc agtggcggcc cttgtcgtcg
     7501 ggcatctccc agaggcgctt gccggcgaag aggatctgct cctccgggtc gaaggcagtc
     7561 tcgatgaagg tcttgtgaaa ttcgcgatgg ttgtccggcg cgaagccgag gttccactcg
     7621 aagtagctga agaggtcgag ttcgcgcgtc cggccgctgc ggttggcgag gtggaggatc
     7681 cagatttcgg ccggctcctt ctctgccgcg aagaggctcc agtcgcaggc gatcccctcg
     7741 tggagggagg agagcaccga atagccgagg ccgtgccgga tgatgtactg gccattcctt
     7801 tttttgaccg gctggaaagc ggccgaccag atctcacccg aggcgcggtc gcggaggtag
     7861 atgaacttgc cccagtcgtc gcgtacgagg tcctggttcc agcgggtgag gcggttaaag
     7921 ttggcgtgcg tccgccagct gaaaccccct ccggcctggg agacgatcag gccatagtcg
     7981 ccgttggaga tgacattgac ccagggcttg ggggtgtcgg ggcgtgtgat gatgtactcc
     8041 aggccgtcat cactgaaatg gccgtagcgg gtggcgaatt tcattgcggc tgctgctcct
     8101 gattccgtct tattcaagtg ccagtccgag cgtgaaacgc tgggtgtttt ccagacggcc
     8161 gtggtccgcc caggcgtaat cggcctggaa gagggcgccg ccgagttcaa agacgatgcc
     8221 ggtaccgagg gtgaggccga cctccgagtc ctgcagccag agattctggt agccggcgcg
     8281 cagggcgagg gtcttcatga agagcc
//
LOCUS       DAOPWU010000143         8228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8701_length_8228_cov_5.601737, whole genome shotgun sequence.
ACCESSION   DAOPWU010000143 DAOPWU010000000
VERSION     DAOPWU010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8701_length_8228_cov_5.601737"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKM23_08575"
     CDS             <1..451
                     /locus_tag="PKM23_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91567.1"
                     /translation="KCYTLPFKGQVEMFRYIFLGTDNVYVAQPGVIYSNLRILGESDT
                     ADVLADRVPVQQPLRARLLPNYPNPFNPTTTLAYEVQESAHVTLQIYNLFGQLVATLV
                     DAERAPGSYEAVWNGEDLLGRPAAGGVYFSRLVTANGQSTRRMTLLK"
     gene            complement(552..2621)
                     /locus_tag="PKM23_08580"
     CDS             complement(552..2621)
                     /locus_tag="PKM23_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNY91568.1"
                     /translation="MNRIIRTTLAMVLLLSAALATADEGMWTFDNPPTKQLQEKYNFT
                     PTKEWLDHVRLSSVRFMDGGSGSFVSPNGLVMTNHHVAMGQLQKMSTAEQNFVATGFY
                     APTPEQEAKCPDLELNVLVEMVNVTSRITGAVKPGMKPADALKARKEEQARIEKEYSD
                     KTGLRCDVVSLYNGGEYWLYQYKRYTDIRLVMAPERQIAFWGGDDDNFTYPRYDLDMA
                     FFRVYVDDQPLSTPDYLKFNAAGAADNELVFVSGHPGSTSRLDTYAMMEMQRDYSYPM
                     SLEMIQKRLDVLRSYSKKGPEQERRALGQIFGLENSKKALGGQYQGLLDAKLMAKAKA
                     DEEAFRARVNANPEWKKAYGDGWKTIEKVIAKQKKEFGSRSYGSLALSSSFARYAQTL
                     VFAAAERKKPDMERLDGYHDVQLERAKLRLFSPAPIYKDLEEAIFTSMLQMAVDKAGT
                     ADPIVSAVLKGRSAEKAAQEMIAATTLDKPDVRKALFEGGAEAVAQSTDPFIVVIREL
                     EPMLRERQEKSRKETESLLTPASEKIANARFAVYGKNMYPDATFTLRLSYGAVKGYPM
                     NGTKAPYKTTLYGLYDRALGFDQEGAWALPPRFWDRQKDLDLSTPANFVCTADIIGGN
                     SGSPVINRNAELVGLVFDGNIESLVGDSVYDENANRCVAVHSAFIIEGLRKLYDAGKL
                     ADEIEGK"
     gene            complement(2695..4665)
                     /locus_tag="PKM23_08585"
     CDS             complement(2695..4665)
                     /locus_tag="PKM23_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNY91569.1"
                     /translation="MLQIRNLSYYIGERLLLKEVNWVINPGKRVALIGPNGAGKTTLL
                     RIIKGELTPHDGAMIKPREYSIGYLPQEEIAVGSGPILATVLEGQGEILLLEEQIAGL
                     HAILNSHTEDDEKLLERLGALETRYDALGGYTIENEAKAILTGLGFSEGDYQRPMSEF
                     SGGWRMRVYLARLLLQNPDLLLLDEPTNHLDLPSLEWLESYLLSFAGSIVLVSHDRFF
                     IDRLAQEITELDRGVLTHYTGNYHLYEKQKAQSEELLLKQWEAMRDERERQQKFINRF
                     RYKATKAAAVQSRIKMLDKMEEVELPPPPPPRIRFRLRVAGSSYKDVLHIEKMGFRYA
                     EPWVLREVDLDIYRGQKVALVGVNGAGKTTLTRLICGEIQPQEGAIRIGERTTIGYYA
                     QHQVDALNLDHTAYEAVASTTAQALAPRIRDILGLFQFHGEDVFKPIRVLSGGEKARV
                     SLAKILLSPVNFLIMDEPTNHLDVRAKEALEEALQEYDGTLLLIAHDRYFLDKLVSRV
                     IELKEGHLTLYEGNYSDYIAKREADAARAAAELQAARMALREAYPEPAAPARKSKEQK
                     RQEAEARQSISRERNRLEEAIARIEAEIQAREEKKSALEAQLANPQSYKNGDFAAQLT
                     RDYAQIKSELEWLVTEWTENQDALEQLLKTLS"
     gene            complement(4900..5619)
                     /gene="prxU"
                     /locus_tag="PKM23_08590"
     CDS             complement(4900..5619)
                     /gene="prxU"
                     /locus_tag="PKM23_08590"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083777221.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5368..5370),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HNY91570.1"
                     /translation="MAEEVKAGCARPTGGPVGETAPPPESAPTAPKEEKKPMLKLGSK
                     APDFTAPAYQQGKFINVKLSDYLGKWVVLCFYPGDFTFVUATEISAVAEKAAEFEKLG
                     VQVLSMSVDSHFVHKMWNDHELSKMVKGGVPFPMLSDGGGKVGAAYGVYDENAGVENR
                     GRFLIDPDGVVVGYEVLTPPVGRNVAETLRQIQAFQLVRASKGTQATPSGWKPGKKVL
                     TPGPDLVGKVWEVWKVSEAFE"
     gene            complement(5755..6936)
                     /locus_tag="PKM23_08595"
     CDS             complement(5755..6936)
                     /locus_tag="PKM23_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNY91571.1"
                     /translation="MPTPTTRMIMLTAIGLLLSALSPAAHAQWKPSPGDTLQTPVFLP
                     GNQVLFRIYAPNAEMVKLAGGDLPSLGWTGKEMTRSAEGIWDITLPVEPGTYRYNFSV
                     DGLTTIDPKNPKTSESNGNTWSLLHQPGAAFMDTRNVPHGALAEVTYWSASLQRFRRL
                     HVYTPPGYENSRKKYPLFYLLHGAGDCDDSWSTVGRAGFILDNLIADGKAVEMVVVMP
                     AGHTGPFIWGAPRGDLAKTMVDEFVMDFTNDIMPLVESRYRVMPGRKHRAIAGLSMGG
                     FQTLNIAIPTVDRFAYAGVFSSGIIGIQPNPATGEATAGPSWEEKYAAQLDNPAVKKG
                     LKLLWFATGREDFLLETSRATIEMLRRHGFTVTYQETAGAHTWINWQLYLNEFAPLLF
                     R"
     gene            7183..7560
                     /locus_tag="PKM23_08600"
     CDS             7183..7560
                     /locus_tag="PKM23_08600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNY91572.1"
                     /translation="MGSENKFNLRRRAASFGYALAGIRQMLQTQHNAWIHLAATVLVL
                     IAAFIYQLSRAEWIAVIAAMALVWSAEAVNTAFEFLCDIVHPGFFPAVEKAKNIAAAA
                     VLICATGAAVIGALVFIPHLLAR"
     gene            complement(7591..>8228)
                     /locus_tag="PKM23_08605"
     CDS             complement(7591..>8228)
                     /locus_tag="PKM23_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNY91573.1"
                     /translation="GATGLVGSHCLRLLLQDSSFTQVIALTRRPLPLSHPRLDARLID
                     FDKLDEALEGVQAESVFCCLGTTIKKAGSQAAFRRVDHDYPLLLAEWAARSGAQQYLL
                     VSAMGADAESRVFYNRVKGETEKAIAAQAIPSITFFRPSLLLGERSERRWAEEAGALL
                     FHLLPFIFRGPLAKYRPNEAEHVAQAMLAHAREAKAGLHIVPAGEMHGEDH"
BASE COUNT         1505 a         2284 c         2706 g         1733 t
ORIGIN      
        1 aaagtgttac acccttccct tcaaaggtca ggtggagatg ttccgttata ttttccttgg
       61 cacggataat gtctatgtcg cccagccggg ggtgatttac agcaatctgc gcatcctcgg
      121 cgagagtgat accgccgatg tattggctga cagggttccg gtgcagcagc ccctgcgggc
      181 gcggctgctg ccgaattatc ccaatccctt caatcccacc accaccctgg cttatgaggt
      241 gcaggagagt gctcacgtca ccctgcagat ttacaacctc ttcggccagt tggtggccac
      301 cctggtcgat gcagagcgcg ccccgggctc ctatgaagca gtctggaacg gcgaagatct
      361 actcgggcgg cccgctgcgg gcggggttta tttctcgcgg ctggtgacgg ccaacggcca
      421 gagcacccgg aggatgacac tgctcaagtg agacggcaac ggaaacaaaa aaaagtgcgg
      481 cgcaacggcg ccgcactttt tttttatcat taaacggaga aatcggggag agaaaaaggc
      541 ttcagtccgg gctatttccc ttcgatctca tcggccagct tgccggcatc atagagcttg
      601 cgcagccctt cgatgatgaa ggcgctgtgc acggcgacgc agcggttggc gttctcatca
      661 tagaccgaat cgccaaccag gctctcgata ttgccgtcaa aaaccagtcc gaccagctcg
      721 gcattgcgat tgatcaccgg cgagccggag ttgccgccga tgatatcagc ggtgcagacg
      781 aaattggcgg gggtggagag gtcgagatcc ttctgacggt cccagaaacg cggcggcaga
      841 gcccaggcgc cctcctgatc gaaacccagg gcgcggtcat aaaggccgta gagggtggtc
      901 ttgtaggggg ctttggtgcc gttcatcggg tagcccttga cggcgccgta ggagaggcgc
      961 agggtgaagg tggcatcagg atacatgttc ttgccataga ccgcgaaacg ggcattggcg
     1021 atcttttctg aggcgggggt gagcagcgac tcggtctcct tgcgggactt ttcctggcgt
     1081 tcgcgcagca tgggctcgag ctcgcggatc acgacgatga agggatcggt cgactgggcg
     1141 accgcctcgg cgccgccttc gaagagagcc ttgcgcacat cgggcttgtc gagggtggtg
     1201 gccgcgatca tctcctgggc agccttttcg gcacttcgcc ccttgaggac ggcggagacg
     1261 atgggatcgg cggtgccggc cttgtcgacg gccatctgca gcatcgaggt gaagatggcc
     1321 tcctccagat ccttgtagat cggggcgggc gagaagaggc gcagcttggc gcgttcgagc
     1381 tggacatcat gataaccgtc gaggcgttcc atatccggtt ttttccgctc ggcggcggcg
     1441 aagaccaggg tctgggcata gcgggcgaag ctggagctca gggcgaggct gccataggaa
     1501 cgggaaccga actccttttt ctgtttggcg atcacctttt cgatggtttt ccagccgtcg
     1561 ccgtaggcct ttttccactc gggattggcg ttgaccctgg cccgaaaggc ctcttcatcg
     1621 gccttggcct tggccatcag cttggcatca agcaaaccct ggtactggcc gcccaaggct
     1681 tttttgctgt tctccagacc gaagatctgg ccgagagcgc ggcgttcctg ctcgggtccc
     1741 tttttcgagt aggagcggag gacatcaaga cgcttctgaa tcatctccag ggacatggga
     1801 tagctatagt cccgctgcat ctccatcatc gcataggtgt cgaggcggct ggtgctgccg
     1861 ggatggccgg agacgaagac cagttcgttg tcagcggcac cagcggcgtt gaatttgaga
     1921 tagtcggggg tgctaagggg ttggtcatcc acatagacac gaaagaaggc catatcgaga
     1981 tcatagcggg gataggtaaa gttgtcgtcg tcgccgcccc agaaggcgat ctggcgttcg
     2041 ggggccatga ccaggcggat atcggtgtag cgcttgtact ggtagagcca gtactcgccg
     2101 ccgttataga gggagaccac atcgcagcgc aggccggtct tgtcgctgta ctctttctcg
     2161 atcctggcct gctcttcctt gcgcgccttg agggcgtcgg ccggtttcat gcccggcttg
     2221 accgcaccgg tgatgcggct ggtgacgttg accatttcga cgagtacgtt cagctccaga
     2281 tccgggcact tggcctcctg ctccggggtg ggggcgtaaa agccggttgc gacgaaattt
     2341 tgctcggccg tcgacatctt ttgcagctgt cccatcgcca catgatggtt ggtcatgacc
     2401 aggccgttgg ggctgacgaa ggagcccgag ccgccgtcca taaagcggac gctggagagg
     2461 cggacgtggt ccagccactc tttggtcggg gtaaaattgt atttttcctg cagctgtttg
     2521 gtcgggggat tgtcgaaggt ccacattcct tcatcggctg tggccagagc agccgagagg
     2581 agcaggacca tcgccagggt ggtgcggatg atgcggttca agtttgcctc ctgtatttat
     2641 tatgagatta ggatttcccc attagagttt agacggatga gaagcgaaaa ggtttcagga
     2701 gagggttttc agcagctgtt ccagggcatc ctgattttcg gtccattcgg tcaccagcca
     2761 ctcgagttcg gatttgatct gggcatagtc gcgggtgagc tgagcagcga aatcgccgtt
     2821 tttgtagctc tgagggttgg cgagctgggc ctcgagggcg ctctttttct cctcgcgggc
     2881 ctggatttcg gcctcgatgc gggcgatggc ctcttccagc cggttgcgct cgcggctgat
     2941 ggactggcgg gcctcagcct cctgccgttt ctgctccttg ctcttgcgcg ccggggcggc
     3001 cggctcggga taggcctcgc ggagtgccat ccgggccgcc tgcagctcag cggccgcgcg
     3061 ggcggcatcc gcttcgcgct tggcgatata gtccgaatag ttaccctcat aaagggtgag
     3121 atggccctcc ttcagttcga ttacccggct gaccagcttg tcgagaaagt agcggtcgtg
     3181 ggcgatgagc aggagggtgc cgtcgtactc ctgcagggcc tcctcaaggg cctccttggc
     3241 gcggacgtcg aggtggttgg tgggctcatc catgatgagg aaattgaccg gtgagagcag
     3301 gattttggcg agcgaaacgc gggctttttc acctccggag aggacgcgga tcggcttgaa
     3361 gacatcctcg ccatggaact ggaagaggcc gagaatgtcg cgaatgcggg gggcgagggc
     3421 ctgtgctgtg gttgaagcca ccgcctcata ggcggtatga tcgaggttga gggcgtcgac
     3481 ctgatgctgg gcgtagtagc cgatggtggt gcgctcgccg atgcggatgg cgccctcctg
     3541 gggctggatc tcgccgcaga tcaggcgggt gagggtggtc ttgccggcgc cgttgacccc
     3601 gaccagggcc accttctggc cgcggtagat atcgaggtcg acctcccgca gcacccaggg
     3661 ctcggcgtaa cgaaaaccca tcttctcgat atgcagcaca tccttgtagc ttgatccggc
     3721 gacgcggaga cggaaccgga tcctgggcgg cggcggagga ggcagctcga cctcctccat
     3781 cttgtcgagc atcttgatgc ggctctgcac ggccgcagct ttggtcgcct tgtagcgaaa
     3841 gcggttgatg aacttctgct ggcgttcccg ctcgtcccgc atcgcctccc actgtttaag
     3901 cagcagctct tcactctgcg ccttctgctt ttcgtaaaga tggtaattgc ccgtgtaatg
     3961 ggtgaggacc ccgcggtcga gctcggtgat ctcctgggcg aggcggtcga tgaaaaagcg
     4021 gtcgtgcgaa accagcacga tgctgccggc gaaggagagc agataactct ccagccattc
     4081 gagcgagggg agatcgagat ggttggtggg ctcatcgagg aggaggaggt cagggttctg
     4141 gagcagcagg cgggcgagat agacgcgcat gcgccagccg ccgctgaact cactcatcgg
     4201 gcgctgataa tccccttcac tgaatcccaa tcctgtcagg atcgccttgg cttcgttctc
     4261 gatggtgtag ccgccgaggg cgtcatagcg cgtttcgagg gcgccaaggc gttcgagcag
     4321 cttttcatca tcctcggtat gggagttgag gatggcatgc agtccggcga tctgttcctc
     4381 gagaaggagg atctccccct ggccctcgag cacggtggcg aggatgggac cgctgcctac
     4441 ggcgatctcc tcctggggta gatagccgat ggagtactcc ctcggtttga tcatggcgcc
     4501 gtcgtgcggc gtcagctcgc ccttgatgat gcgcagcagg gtggtcttgc ccgcgccgtt
     4561 ggggccgatg agggccaccc gcttgccggg attgatgacc cagttgacct ccttgagcag
     4621 caggcgttcg ccaatataat aggaaaggtt tctgatctga agcatgttgg gcaaataggg
     4681 tcaggatgat atggccatat cgctcgcatt ccgcggcggt gggattgaaa atcaatccat
     4741 taaaatagca aaaaataggt tgggaaaaaa cataattctt gggctgccgg gaggggcagg
     4801 agcgcgccgg ggcttgcgga gagggttctt cggggtgagg ggtaaaaaaa atcccgcccg
     4861 aaactgccgg gcgggaagtg gggccagtgt gatagagctc tattcgaagg cttccgaaac
     4921 cttccagacc tcccagacct tgccgaccag atcggggccg ggagtgagga ccttcttgcc
     4981 cggcttccaa cccgaagggg tggcctgggt gcccttgctc gcgcgtacga gctggaaggc
     5041 ctggatctgc cggagggttt cggcgacatt gcgtccaacc ggcggagtga gcacttcgta
     5101 gccgacgacc acgccgtccg gatcgatcag gaatctgccg cgattctcaa cgccggcatt
     5161 ctcgtcgtat acgccgtagg ctgctccgac cttgccgccg ccgtcggaga gcatggggaa
     5221 gggaacgccg cctttgacca tcttggagag ctcgtggtcg ttccacattt tgtgaacaaa
     5281 gtggctgtcg acgctcatgg agagcacctg cacacccagt ttctcgaatt ctgctgcttt
     5341 ttcggcgacc gccgaaattt cggtcgctca gacaaaggta aaatcgcctg gatagaagca
     5401 gagaacaacc cacttcccca ggtagtctga cagcttgaca ttgatgaatt tcccctgctg
     5461 gtaggccggg gcggtgaaat cgggggcctt gcttccgagt ttaagcatgg gctttttctc
     5521 ctctttgggg gctgttggag ccgattcggg aggcggagcg gtctctccga caggtcctcc
     5581 tgtcggtcgc gcgcagccag ccttgacttc ttctgccatg ggctttcctt tcaaattagt
     5641 gtcaacaggt gtgccttgca gaggcagagt ggatgatgtg taatcttacg aatatctcct
     5701 tacaacgaca aaccttttat tttttcgccc tgaggcgagc cggagggcgt ggctttatcg
     5761 aaagagcaga ggggcgaact cgttgagata gagctgccag ttgatccagg tatgggctcc
     5821 ggccgtctcc tggtaggtca cggtaaagcc gtgcctgcgc agcatctcga tggtggcgcg
     5881 ggaggtctcg agcagaaaat cctcgcggcc ggtggcgaac cagagcagtt taagcccttt
     5941 tttgacggcg ggattatcga gctgggcggc gtatttctcc tcccaggagg gtccggccgt
     6001 ggcttcgccg gtagccggat tgggttggat gccgatgatg cccgagctga agaccccggc
     6061 ataggcgaac cggtccacag tggggatagc gatgttgagg gtctgaaaac cgcccatgga
     6121 gaggccggcg atggcgcgat gtttccggcc gggcatgacc cggtagcggg actcgaccag
     6181 gggcatgatg tcgttggtga agtccatgac gaattcgtcg accatggtct tggcgaggtc
     6241 accgcggggg gcgccccaga tgaaggggcc ggtatggccg gcgggcatga ccaccaccat
     6301 ctcgactgcc ttgccgtccg cgatcaggtt gtcgaggatg aagccggcgc ggccgacagt
     6361 gctccaggaa tcatcgcaat ccccggcgcc gtggagcagg taaaagaggg gatacttttt
     6421 cctgctgttc tcatagccgg gcggtgtata gacgtgcagg cggcggaagc gctgcagcga
     6481 ggcggaccag taggtcacct cggcgagggc gccgtggggg acattgcggg tatccataaa
     6541 ggccgcaccc ggctgatgga gcaggctcca ggtattgccg ttcgattcgc tggttttcgg
     6601 atttttgggg tcgatggtgg tgaggccgtc gacactgaaa ttgtagcggt aggtgcccgg
     6661 ttcgacgggc agggtgatgt cccagatgcc ttcagcggag cgggtcatct ccttgccggt
     6721 ccagcccagg ctggggagat ccccgcctgc cagtttcacc atttcggcgt tgggggcata
     6781 gatgcggaag agcacctgat ttccgggcag aaacaccggg gtctgaaggg tgtctccggg
     6841 tgagggcttc cactgggcat gggcggccgg cgagagggcg gacaacagga ggccgattgc
     6901 cgtcagcatg atcatacggg tggtgggtgt gggcatgggg tgactccctg atttgaaatg
     6961 aacagcaatg tagattatgg cggggttggg ttactgcgga cgggcttgcc ccgcagcggc
     7021 cgtccggcct tccaatttag tacctctccg gcgaaaaaac aagtccaatc aggtggaggc
     7081 ggcgccttcc ttgcgggcag ttttgccttg actgtaagtg cagagattgc taatttgcgc
     7141 ctgtaaagag aacaccaggt ttagcatagg cggggcctgc ccatggggtc ggagaacaag
     7201 ttcaatctgc gccgccgcgc ggcgagtttc ggttatgcct tagccgggat ccggcagatg
     7261 ctgcaaaccc agcacaatgc ctggatccat ctggctgcca cggtactggt cctcatcgcc
     7321 gcttttatct accagctctc cagggctgaa tggatcgcgg tcatcgccgc catggccctg
     7381 gtctggtcag ccgaggcggt caataccgcc tttgaattcc tctgcgacat cgtccatccc
     7441 ggcttctttc cggctgtaga gaaagccaaa aacatcgccg ccgccgcagt tctgatctgt
     7501 gcgaccggcg cggcggtcat cggcgcgctg gtattcattc cccatcttct ggcccgctaa
     7561 ggctaccggt catcagcagt acggactccc tcagtgatct tcgccgtgca tctcgccggc
     7621 cgggacgatg tgcagcccgg ctttcgcctc gcgggcatgg gccagcatgg cctgggccac
     7681 gtgctctgct tcattcggcc ggtatttcgc caggggaccc cggaaaataa aaggcagcag
     7741 gtggaagagc agcgccccgg cctcctcggc ccagcggcgt tcactgcgtt cacccagaag
     7801 cagcgagggg cggaagaagg tgatggaggg gatggcctgc gcagcgatcg ccttttcagt
     7861 ctcccccttg acccggttgt aaaagactct cgattccgcg tccgcgccca tggcgctcac
     7921 gagcaggtac tgctgcgcac cgctgcgagc cgcccactcc gccagcagga ggggatagtc
     7981 atggtccacg cgccggaatg cggcctgcga acccgctttc ttgatggttg tgcccaggca
     8041 gcagaagacg ctctccgcct gaaccccctc cagggcctcg tccagcttgt caaaatcgat
     8101 cagacgcgca tcgaggcgcg ggtggctgag cggcagggga cggcgcgtca aggcgatcac
     8161 ctgcgtgaaa gaggagtcct ggaggaggag ccgcaggcag tggctcccga caaggccggt
     8221 cgctccgg
//
LOCUS       DAOPWU010000144         8167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8783_length_8167_cov_4.932446, whole genome shotgun sequence.
ACCESSION   DAOPWU010000144 DAOPWU010000000
VERSION     DAOPWU010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8783_length_8167_cov_4.932446"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="PKM23_08610"
     CDS             <1..1877
                     /locus_tag="PKM23_08610"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNY91574.1"
                     /translation="DEVSRVFQESGIILSEEDIVNLMSGSAGGEGGSQENRRGYQLRA
                     IAAAVEKLLRESYTAGILSVERSAFSPLPARFSIRYRGLEVFENSEFILGLHELRQQV
                     PEKLRISQQLDDAQSKIGYKMALAFLSPNLIYDQAETQTRMEEAVALVPLAKDQVLAG
                     ERIIDGHERITREHIEKLNSYAAAKTERGEGKGLISRLLHGLGQWGNSLFILSILLFF
                     LWQGRGEIANAPKQLLLIALIVLLPAGLTFLVTQLNLSPLLIPVAAGAMIITIFFDVY
                     VGLLYAISVSLLVGAMRGNEFGIAYISIFVSAMAILSVSRVRTRNWVLRSMGVVMAAY
                     LLSITVHDLLNYQSFQEILRDWGLGFINGFISPIIAYALVLLLERTFHLTTDMTLLEL
                     SDLNHPLLRQLALQAPGTYHHSLSVGNLAETAAEAIGANALLARVGAYYHDIGKLEKP
                     EYFVENQMRGRNPQEKLTPTMSSLILQNHVRRGAEMAAQYHLPPAVSAFIEEHHGTSL
                     MHYFYQKAVEQKGEGAVPQDEFRYPGPRPSIRESAIVMLADAVEATSRTLRDPSYSRI
                     KNIVDQIIDERFKSGELDNSPLTLQDLARISEAFQKTLNARFHRRIPYPNQNTDDEGE
                     "
     gene            1877..2335
                     /gene="ybeY"
                     /locus_tag="PKM23_08615"
     CDS             1877..2335
                     /gene="ybeY"
                     /locus_tag="PKM23_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNY91575.1"
                     /translation="MSKPTLPRQVSLVDGCKKPEISAGELTLIAELILEQARPQGGWR
                     VQIIVVPEEEIIRLNRRIFQRSGSTDVISCNLTTAEEPVLEGEVYISLETALRQAREY
                     RVSAGEELQRLTAHGIFHLLGYEDGDVRQKEIMTALEDEAMRRLRAFGRK"
     gene            2413..3582
                     /locus_tag="PKM23_08620"
     CDS             2413..3582
                     /locus_tag="PKM23_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91576.1"
                     /translation="MVSLRFDFRDLFRAPRLAFSLQRMWILLVGIAAGWLTYFLLTFL
                     ALLLAGPAPGEIWSRFGLLPALSASRYSFPWYSWLLQGLAAAAAFCALLIAQTAHARA
                     LYMSCKGEHFYSWRKAYAFAWRKLGAVIMTPTVLGLLMLLFIGGAWLAGLAGRIPWAG
                     ELGIALLAVIWFVLALLMIFFGMVLLVALLYAPAVIAATDEDAFESIFQLFSLVWNQP
                     WRLLIYELLSVLLALFALGVLAFFCKRAVGLTNSLFSYFMGGNYADLANNGQALVQAW
                     TAAGEGMLFWLFRGFTPLLYFTQEFYYLPVQELARPTVAVSGYLYAFSLLFLAGWVFS
                     YGLSTLNAAHLLSYLSMRKHKDEVNLLERRDREEEYEEELESEADGKEPPPAQNQ"
     gene            3738..5021
                     /locus_tag="PKM23_08625"
     CDS             3738..5021
                     /locus_tag="PKM23_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNY91577.1"
                     /translation="MSVFIKIKDQQLGPYSYNELKKLVTEGSFDQDDLVFSEASNEWV
                     RAGHLEELRKLFPGPVSTPSERMVYAIGGGKGGVGKTVLTASIGIGLAALGYRVVMVD
                     ADLGGANLHTCMGILEPQYTFYHFYSLQKESLSDILLDTPIENLKLISGACGTLGLAN
                     PRYSQKLRFINELRKIDADFILLDLGAGSSYNVIDFFLAADHGLAVTTPEPMAIQETF
                     NFLKIALMRKLTREFKDDPDAINRLEQEVFADSGRMSQSVGAALQKLAGEDPAAAKRI
                     QGILDGFTPSLILNMVHSEDEVKEGVALRTAVEELLAMKMNFLGYVEHDESVRRAVKE
                     LRPFVVDDPTSRAAKNLAKLITVGLLKKTGWAGFKEKRRVLRQVKQQNKEYPSQQIRE
                     SETICSVQCFYWGDCEYQNGGYPCPVRHLDPIFKS"
     gene            5108..6478
                     /locus_tag="PKM23_08630"
     CDS             5108..6478
                     /locus_tag="PKM23_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNY91578.1"
                     /translation="MKATLQFWQKIARQFHPAHILALSFLFLILVGTLLLALPVSKLD
                     PNARLIDALFISTSATCVTGLAPLDISATYTFFGNVVILLLIQFGGLGIMTFSTFFAY
                     ILAGRLSMRGRDLIENSISSQPVPYLGRLLKFAVIGTLLIEGIGAVILTLRFSQDYRL
                     GKALWLGIFHSVSAFCNAGFSPFNSNMMGYASDVTISLTVMVLIVLGGLGFWVLYDLF
                     YWRPRGRKRLTLHSKIALGVSGWLILIGACLLLFFEGDNTMRGFDAGGKLLSSFFQSI
                     TARTAGFNSLPIDKLTNGALIVLIILMAIGASPGSTGGGIKTTTFAIIMASFFTRLRS
                     KEQVRIFERGIPEQIVTKAIGISFFWVMAVSLAALLMAVTESSGPPDPLARNTMVEIV
                     FEAFSALGTVGLSMGFTPRLSDGGKLIIILLMYIGRIGPVTLAISIASQKSLPVRYAE
                     ENVIVG"
     gene            6500..7156
                     /locus_tag="PKM23_08635"
     CDS             6500..7156
                     /locus_tag="PKM23_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNY91579.1"
                     /translation="MKSFAIIGLSSFGHYLAKELSHRGHHILAIDIDEEKIDKVRGFV
                     ERAVIADAGDKETLEGLGLAELDGVIVSMGEKIDASILVTLFLRELKVKKIIAKAITN
                     DHGRILDIIGATQVVFPERDEAMRLAHTLESEYYLHAINLTEGISIIEIAPPKPFVAK
                     SLGELDLRNRYGVTVLLVKEWIPEKVVLIPTADWVIKDSDTLVLLGKDEDLDRIKEIS
                     "
     gene            7270..7713
                     /locus_tag="PKM23_08640"
     CDS             7270..7713
                     /locus_tag="PKM23_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNY91580.1"
                     /translation="MPIQVLRVIRDMSDRGGLRSRRCTYYCPEAEAPVSSWWKPNTDI
                     LESEQQVKIRVELAGVARENVNIELKGGRLIISGVRAERRPEERIYYHQLELQYGHFL
                     RVIALPESVVHNEITATLEDGVLQVVISKDERAVEIPISSIEPDE"
     gene            7760..>8167
                     /locus_tag="PKM23_08645"
     CDS             7760..>8167
                     /locus_tag="PKM23_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNY91581.1"
                     /translation="MDDNMDVPAGVAGEEINVARIPEQLPILPLRDTVVFPNIVTPLV
                     VAREKSVQLINDVIAGSRILGLVAQREAEVEDPGLKDVFEYGCAAIILRMLKFPDGSL
                     RVLVQGLSRIHVKRLVQETPYFIGEVEKLEERYT"
BASE COUNT         1562 a         2390 c         2433 g         1782 t
ORIGIN      
        1 ccgacgaagt gagccgggtc ttccaggaga gtgggatcat cctctccgag gaggatatcg
       61 tcaatctcat gtccggttcc gcgggcgggg agggcggctc ccaggagaac cgcagggggt
      121 atcagctccg cgctatcgct gcagcggtcg aaaagctgct gcgcgagagt tacaccgccg
      181 gcatcctcag cgtcgagcga agcgcttttt cccccctgcc ggcccgcttc tccatccgct
      241 atcgcggcct cgaggtgttc gaaaacagcg agttcatcct cgggcttcat gagctgcggc
      301 agcaggtccc cgagaagctg cgcatcagcc agcagctgga cgatgcacag tccaagatcg
      361 gctacaagat ggcactggcc ttcctctcgc cgaacctgat ctatgaccag gcggagaccc
      421 agacgagaat ggaggaggcc gtcgccctgg tgccgctcgc caaggaccag gttttggccg
      481 gggagcgcat tatcgacgga cacgagcgca tcacccgcga gcatatcgaa aaactaaact
      541 cctatgccgc cgccaagacc gagcgcggcg aggggaaggg ccttatcagc cgccttctgc
      601 atggcctggg ccaatggggc aactcgcttt tcatcctcag catcctgctc tttttcctgt
      661 ggcagggaag gggggagatc gccaacgccc ccaaacagct gctgctcatc gccctgattg
      721 tgctgctgcc ggccgggctc acttttctgg tcacccagct caatctctct ccgctgctca
      781 tcccggtggc ggcgggggcc atgatcatca cgatcttttt cgatgtctat gtggggctgc
      841 tctacgccat ttcggtcagt ctgctggtcg gggcgatgcg cggcaatgag ttcggcatcg
      901 cctatatctc catctttgtc agcgccatgg cgatcctctc ggtcagccgg gtgaggacgc
      961 gcaactgggt tttgcgtagc atgggggtgg tgatggccgc ctatctgctc tcgatcaccg
     1021 tgcacgacct gctcaactat caatccttcc aggaaatcct gcgcgattgg gggctgggct
     1081 tcatcaacgg cttcatctct ccaatcatcg cttatgccct ggtgctgctg ctcgagcgca
     1141 cctttcacct caccactgac atgaccctgc ttgagctttc cgacctcaac catccccttt
     1201 tgcgacagct ggccctgcag gcccccggga cctatcacca ctccctttcg gtaggaaatc
     1261 tcgccgaaac ggcggccgag gcgatcgggg cgaacgccct gctggccagg gtgggcgcct
     1321 attaccacga catcggcaag ctggaaaaac cggaatattt tgtcgagaat cagatgcgcg
     1381 gacgtaatcc ccaggaaaag ctgaccccga cgatgagctc cctgatcctg cagaatcacg
     1441 tccggcgcgg cgcggagatg gccgcccagt atcacctccc tccggcggtc agcgccttca
     1501 tcgaggagca tcacggcacc agcctgatgc actatttcta ccaaaaggcc gtcgagcaga
     1561 agggggaggg ggcggttccc caggatgaat tccgctatcc cggcccccgc ccgagcatcc
     1621 gcgaatcggc catcgtcatg ctcgccgatg cggtcgaggc cacctcgcgc actcttcgcg
     1681 atccctccta cagccggatc aagaacatcg tcgaccagat catcgatgaa cgcttcaaga
     1741 gcggcgaact cgacaactcg cccctgacct tgcaggatct cgcgcggatt tcggaggcct
     1801 ttcagaagac cctcaatgcg cgctttcacc gccgcattcc ctatcccaac cagaacacgg
     1861 acgacgaggg ggagtgatga gcaagccgac tctgccgcgg caggtgagcc tcgtcgatgg
     1921 ctgcaagaag ccggagatct ccgccggtga gctgaccctg atcgcagaac tcattcttga
     1981 gcaggcccgc cctcaggggg ggtggcgggt gcagatcatc gtggtgccgg aggaggagat
     2041 catccggctc aaccggcgca tcttccagcg cagcgggagc acggatgtaa tctcctgcaa
     2101 tctgaccacg gcggaggagc ctgtgctgga aggggaggtt tatatcagcc tcgagaccgc
     2161 cctgcggcag gcccgggaat accgggtgag tgcgggcgag gagctgcagc ggctgactgc
     2221 gcacggcatc ttccacctcc tcggctatga ggacggagat gtccgccaga aggagatcat
     2281 gactgccctc gaggatgagg cgatgcgacg cctgcgcgcc tttggccgga aatagcgaaa
     2341 ccccgttgga tttttgtaaa aaatgtggta tactatggtg gcgcatcgca caccggagat
     2401 ttgggaggag ttatggtatc gttgcggttt gattttcgcg atctttttcg tgcgcctcgc
     2461 ctggcgttca gcctccagcg gatgtggatc ttgctggtcg ggatcgcggc gggatggctg
     2521 acctattttt tgctcacctt tttggccctg cttttggccg gccctgctcc gggggaaatc
     2581 tggagccgat ttggtctttt gccagcgctt tccgcctccc gatattcttt cccctggtac
     2641 agctggcttc tgcaggggct ggcggccgcg gccgcctttt gtgcgctgct gatcgcccag
     2701 acggcgcatg cacgcgccct ctacatgtcg tgcaagggcg aacatttcta cagctggcgc
     2761 aaggcctatg ccttcgcctg gcgcaagctc ggagcggtga tcatgacccc gacggtgctc
     2821 gggctgctga tgctgctctt catcggcggt gcctggctgg ccggtctggc ggggcggatt
     2881 ccctgggccg gggagctggg cattgctctc ctggcggtta tctggttcgt cctcgccctg
     2941 ttgatgattt tcttcgggat ggttttgctg gtcgcgttat tgtacgcccc tgctgtgatc
     3001 gccgcgaccg acgaggatgc ctttgaatcg atttttcaac tcttttcgct ggtctggaac
     3061 cagccctggc ggctgctgat ctacgagctg ctctccgttc ttctggctct ctttgcactc
     3121 ggcgttctcg cctttttctg caaacgggct gtggggctca ccaactctct tttctcctat
     3181 ttcatgggcg gcaactatgc cgacctggcc aataacggcc aggcgcttgt ccaggcgtgg
     3241 accgcagcgg gagaggggat gctcttctgg ctctttcgcg gctttacccc cctgctctat
     3301 ttcacccagg agttctacta tctgccggtg caggagctgg cgcggccgac ggtggcggtt
     3361 tccgggtatc tttatgcctt cagcctgctc ttcctggcgg gatgggtatt ttcctatggc
     3421 ctttcgaccc tcaatgcggc ccatctcctc tcctacctct ccatgcgcaa acacaaggat
     3481 gaggtgaatc tgctcgagcg cagggaccgg gaggaggagt acgaggagga gctggagagc
     3541 gaagcggacg gcaaagagcc gccgccggca caaaaccagt gaggagttga aaaatatttc
     3601 cgcaaatgtg atgcttgaca cctaattttc agtagctaca tgaagcaagg aggttgccgg
     3661 gggtgccgca tttcccgcac ggacagcgtc tgcacaggct gcatcatcgc actatttgac
     3721 gaaagcaaga gagcatcatg tccgtcttca tcaagatcaa ggatcaacag ctaggcccgt
     3781 actcctataa tgaattaaaa aagctggtca cggaaggttc attcgaccag gacgacctgg
     3841 tcttcagcga ggccagcaat gaatgggttc gtgccggcca tctcgaggag ctgcgcaagc
     3901 tttttccggg gccggtgagc acaccctccg agcgtatggt ttacgccatc ggcggcggca
     3961 agggcggggt gggcaagact gtgctcacgg cctcgattgg catcggcctg gcggcattgg
     4021 gttaccgcgt ggtgatggtg gacgccgatc tggggggcgc caatctccac acctgcatgg
     4081 gaatccttga accgcagtat accttttacc atttctattc cctgcaaaaa gagagtctga
     4141 gcgatatcct tctcgatacc cccatagaga atctcaagct gatcagcggc gcctgtggca
     4201 ccctgggcct cgccaatccg cgctattccc agaagctgcg tttcatcaat gagctgcgca
     4261 agatcgatgc ggactttatc ctccttgatc tcggggcggg ctcgtcctac aacgtcatcg
     4321 actttttcct tgcagctgac cacgggctgg cggtgacgac gccggagccc atggcgatcc
     4381 aggagacctt caattttctc aagatcgcgc tcatgcgcaa gctcacgcgc gaattcaagg
     4441 atgatccgga tgcgatcaac cggctggagc aggaggtctt tgccgattcg gggcggatgt
     4501 cgcagtctgt cggcgccgcg ctgcaaaagc ttgccggaga ggatcccgct gcggcgaaga
     4561 gaatccaggg tattctggat ggctttaccc cctcgttgat cctgaacatg gtccattccg
     4621 aggatgaggt caaggagggg gttgccctgc gcacggcggt cgaggagctg ctggcgatga
     4681 agatgaattt tctcggctat gtcgagcatg acgagagcgt gcgccgggcg gtcaaggagc
     4741 tgcgcccctt cgtggtggat gaccccactt cgcgggcggc caaaaacctg gccaagctga
     4801 ttaccgtcgg tctgctcaaa aagacgggct gggcgggttt caaggagaag cgccgggtgc
     4861 tgcgccaggt caagcagcag aacaaggagt atccctccca gcagatccgg gagagcgaaa
     4921 ccatctgttc ggtgcagtgt ttttactggg gggactgtga gtatcagaac gggggctatc
     4981 cctgtccggt ccgccatctc gatcccatct tcaagtcatg atttaaattt gcttttttct
     5041 tttcccattt ttatgtatac tatgaggtga acaggcgact gttcaccttt tttatatgaa
     5101 ggaactcatg aaggcaacac tgcaattctg gcaaaaaatc gcccgccagt ttcatccagc
     5161 ccatatcctg gcgctcagct ttctcttcct gattctggtt gggacgctgc tgcttgccct
     5221 gccggttagc aagctggacc ccaacgcacg tctcatcgat gccctcttca tctcgacatc
     5281 ggcgacttgt gtaaccggcc tggctccgct ggacatctcc gccacttaca cctttttcgg
     5341 caacgtggtt atccttctgc tcatccagtt tggcgggctg gggatcatga ccttttccac
     5401 tttttttgcc tacattctgg ccggacggct gagcatgcgc gggcgcgatc tgatcgagaa
     5461 cagcatcagc agccagccgg ttccctatct gggccgcctg ctcaaatttg ccgtcatcgg
     5521 caccctgctt atcgagggga tcggggcagt cattctgacc ctgcgtttca gtcaggatta
     5581 ccgtctgggg aaggccctgt ggctcgggat ctttcacagc gtctcggcct tttgcaatgc
     5641 cggcttttcg ccctttaatt cgaacatgat gggctatgcc tccgatgtga ccatcagcct
     5701 cacggtgatg gttctgatcg tactcggggg attgggattc tgggttctgt atgatctttt
     5761 ttactggcgg ccccgggggc gaaagcgcct caccctgcac agcaagatcg ccctgggggt
     5821 ttccggctgg ctcatcctca tcggcgcttg cctgctgctt ttttttgagg gcgataacac
     5881 gatgcggggc tttgatgccg ggggcaagct gctcagctcc tttttccagt ccatcaccgc
     5941 ccggacagcg ggttttaact ccctgccgat cgacaagctg accaatggag cactgatcgt
     6001 gctcatcatc ctcatggcca tcggcgcatc gccgggctcc accggcggcg ggatcaagac
     6061 cacgaccttc gccatcatca tggcctcctt tttcacacgg ctgcgctcga aggaacaggt
     6121 gcgcatcttc gaacgcggca tccccgagca gatcgtcacc aaggcgatcg gaatcagctt
     6181 tttctgggtg atggccgtca gcctggccgc cctgctcatg gccgtcaccg agagcagcgg
     6241 tccccccgat cccctcgccc gcaacaccat ggtcgagatc gtcttcgagg ccttttcagc
     6301 cctcggcacg gttggcctga gcatgggatt caccccacgc ttgagcgacg gggggaaact
     6361 gatcatcatc ctgctcatgt atatcggcag gatcggaccg gtgacgctgg ccatttcgat
     6421 cgcctcgcag aagagcctgc cagtgcgcta tgcggaagag aatgtaatcg tcggttaaca
     6481 ttacatagaa acggaaacca tgaaatcctt tgccattatc ggactaagca gcttcggcca
     6541 ttatctggcc aaggagctca gccatcgcgg ccaccacatc ctcgccatcg acatcgatga
     6601 ggagaaaatc gacaaggtgc ggggatttgt cgagcgggcc gtcatcgccg acgccggaga
     6661 caaggagacc ctggagggtc tgggtctggc cgagctggac ggcgtcatcg tgagcatggg
     6721 cgaaaagatc gatgccagca ttctggtcac cctcttcctg cgcgaactca aggtgaaaaa
     6781 gatcatcgcc aaggccatca ccaatgacca tggcaggatc ctcgatatca tcggcgctac
     6841 acaggtggtc tttccggaaa gggatgaggc gatgcggctg gcgcataccc tggagagcga
     6901 gtactatctc cacgccatca accttaccga gggcatcagc atcatcgaga tcgctccgcc
     6961 caagcctttc gtcgccaaat ccctcgggga gctcgatctg cgcaaccgct atggagtcac
     7021 cgtgctcctg gtcaaggagt ggatccccga aaaagtggtt ctgatcccca ccgctgactg
     7081 ggtgatcaag gacagtgata ccctggtact tttaggcaag gatgaggacc tcgaccggat
     7141 caaggagatc agttaggccc ggcgccgtcc ggcgggaata tttgagcgga aaacggaatt
     7201 atctctcttg cagccgcagg gctgcgataa caggagcgtt tggggggaga gtcatggagg
     7261 cctgttgaca tgcccataca agtattgcgt gttatccgcg acatgagcga tcgcggcggg
     7321 ctgaggagcc gcagatgcac ctactactgt ccggaggcgg aagccccggt cagctcctgg
     7381 tggaaaccga acaccgatat cctcgagagt gagcagcagg tgaagatacg ggtcgagctc
     7441 gccggtgtcg cccgggagaa tgtgaacatc gagctcaagg gcggcaggct gatcatcagc
     7501 ggggtccgcg cagaaaggcg gccggaggag cgcatctact atcatcagct cgagctgcag
     7561 tacggccatt ttttgcgggt catcgctctt cccgagagcg tggtacacaa tgaaattacc
     7621 gccacgctcg aggatggggt gctgcaggtg gtcatctcca aggatgagcg ggctgtcgag
     7681 atcccgatca gcagcatcga gccggatgag tgagcggcga ccgggggctt tattgaaact
     7741 ggatgagatt ggagcagata tggatgataa tatggatgtg ccggctggag ttgcaggtga
     7801 agagatcaat gtggccagga ttccggagca gctgccgatc ctgcccttgc gtgatacggt
     7861 ggtttttccg aatattgtca cccctctggt ggtggcgcgg gaaaagtctg tccagctgat
     7921 caacgacgtc attgccggtt cacggatcct cgggctggtc gcccagcgtg aggccgaggt
     7981 agaggatccg ggactcaagg atgttttcga atacggttgt gcggcgatca tcctgcgcat
     8041 gctcaaattt ccggatggct ccctgcgcgt tttggtccag gggctttcgc gcatccatgt
     8101 caagcggctg gtgcaggaga ccccctattt catcggggag gtggagaagc tggaagagcg
     8161 ctacacc
//
LOCUS       DAOPWU010000145         8139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8829_length_8139_cov_4.986145, whole genome shotgun sequence.
ACCESSION   DAOPWU010000145 DAOPWU010000000
VERSION     DAOPWU010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8829_length_8139_cov_4.986145"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(362..1675)
                     /locus_tag="PKM23_08650"
     CDS             complement(362..1675)
                     /locus_tag="PKM23_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNY91582.1"
                     /translation="MKRFVVLIWLVTLSVALRAQPADFVKVKGTQFYLKGKAYYFLGT
                     NLWYGCMLGQEGEAGDRARLLRELDELKKLGIDNLRVMGASEGLGYAGQIQPAIQPEA
                     GVYDQKLLVGLDFLLAEMAKRGMYAVIFLNNYWEWTGGMSQYMSWVTGENYPSPQDPK
                     FGYWGLMRTSGRFYTCDRAKPIYRDYIRMLVNRVNSVNGRWYKEDPTIMAWQLANEPR
                     PSPEEENRAQHLADFAVWVDETAGYIKSLDPNHLVTTGNEGLKGSLESESCYLESHQS
                     PHIDYLTFHLWLLNWSWFDPLKPGEIWPDAARKALDYVRQHIAYAEKLGKPVTLEEFG
                     LPRDGHAYARTAGVTWRDRYYKLLFEEMYKSAKAGSPMAGSNFWGWGGEGRAADSETY
                     TWRKGDPYTGDPPQEPQGRNSVFNSDKSTLKILRKYAKKMAALGK"
     gene            complement(1743..4832)
                     /locus_tag="PKM23_08655"
     CDS             complement(1743..4832)
                     /locus_tag="PKM23_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNY91583.1"
                     /translation="MRKLVLLTTLMIALIALFPMHIILSGQVSVKGSIREIPTRKKPP
                     LLPDGKWREAKSAYPRSSNLQSGLMNSRLQNAVAGTATLEWAKHYGSGANESIDEATA
                     IAVDKSGNFFVTGRSTQLSFGADYCTVKYDASGTEIWVARYDSGYEDHSVAVAVDGTG
                     NVYVTGYSASPSSSWDFITIKYDADGREKWVRRYNGPGDSDDQPSALSVDSSGYVYIT
                     GYSTGSDSYNDYATIKYTADGDQLWVARYNGALNSYDGASALVVDGTGNVYVTGYSGG
                     SATSNDYTTIKYDAAGVQVWVSRYDGPLSSDDEANALAVDKSGNVYVTGSSNGLGTYS
                     DYATVKYDAAGVQQWVARYNGPGNSHDGGYALAVDGSGNVFVTGFCEDSTSYLDFATI
                     KYDQAGNEKWAVSYDGPGNSADYASFIYLDGSGNAYVAGSSIGFQTSKDYTTIKYDAA
                     GNEQWVARYNGPGYSDDQPGAFAADESGNIYLTGYSTGLDTYYDYATVKYSPDGNEQL
                     AVRYNGLGNSDDEARALAVDNSGNVIVTGHSLSSDTYKDIVTVKYDAAGSEKWMMRFD
                     GSSSSDDEPNALVVDGSGNVYVAGYSVDYENSNDFATIKYSENGDQVWLARYNGPFNS
                     YDGVNALALDGSGNVYVTGYSVGADTSYDYATIKYDAAGAEQWVARYNGPGKDDDVAN
                     AIAVDDLGFVYVTGHGTGSDTTFDITTIKYNSSGVEQWVASYNGPGHADDEAFDLAVD
                     TAGNIYITGYSTGSGTSYDITTIKYDAGGSELWVMRYDGPLSSYDAANALAVDASGNV
                     YVTGYSGGAYTSYDYTTIKYDAAGYEQWVARYNGARNSYDYANALVVDDLENVYITGT
                     CVGSNNYYEYATIKYDAAGNEQWTLLFDLPGYNDSGSDIAVDATGTVYVTGTSDNSFG
                     TSAYTTLKYKQNPTLAEQSFTSMPQECALDQNHPNPFNPSTRITYYLASPVMVTLTVY
                     DLLGREVAVLVNKLQLAGEHTANFNLPEVGRELASGLYFYRLKAGRFIEQKKMLLVK"
     gene            complement(5006..6793)
                     /locus_tag="PKM23_08660"
     CDS             complement(5006..6793)
                     /locus_tag="PKM23_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNY91584.1"
                     /translation="MKKILSTVLLAALWVTTLSGQDRVIRVACIGNSITIGSGGSTAW
                     PQQLGQRLGAHYNVRNFGVSGTTLLRHGDFPYWNESAFLQAQDFDPHIVIICLGTNDS
                     KPQNRVLLGEFFTDYMDFIKVLRKDGRDPQIYVARPCPVFGDGGGSGINGAVLHGQIE
                     PIVDSVRVAAGGFMIDFYRAMLSHGDLFPDGIHPNTTGYAMMADTAAWYLNNSPSGFI
                     RLFAATDTEVEVGETSQLYWEATPGSQVTLNGAPVGASDSLVVAPAAKSIYTLIASGA
                     VADTKRVAIDNIPPGRIKSLSAFPLQLDEGAGDTSRISWMTTNGSSVTLDGAAVAQNG
                     SVAVVPARTTTYTLAAGGGDSQSARITIEVLPAAVINRALRHPLTASSTLRGGNAQWA
                     VDGDTTTAWQSAGKITEWIMVDLGRMITLKTLRIHWGNSFAVSYTVHLLNADGTAIKA
                     ITQRNGDGGLDEIIDIDTQARYVRLLCQKVSGTNYSVTEFELYGTTMPVGVDRHDGVP
                     ALFELEQNYPNPFNPVTTIAYRLTRPAPIRLVIYDVSGRAVRTLDPGIQAEGRHEIIV
                     DASGFSSGLYFYQLTAGLERRQRRMMLIK"
     gene            6881..7963
                     /locus_tag="PKM23_08665"
     CDS             6881..7963
                     /locus_tag="PKM23_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HNY91585.1"
                     /translation="MHKSLCLLLPLLFASACTRPAASSWDAVVDAGYRGKPGAKLAGV
                     PCYPSLSQALAAAPPGETPWRIFMRKGRYHEKVTIDRANLHLRGENREEAILSFDDCS
                     GTPAPGGSTLGTRGSATLTIRAPGFSAEHITIENCFDYPANALRAENDPAKIAHPQAV
                     AVMISAGGDRISFADCVISGFQDTLFPDAGRSLFSRCCILGHVDFIFGAGQALFEQCE
                     IVSRDRPGKNPSGYITAPSTSAAYPYGFLFIACRLLKQGPGVTAGSVRLGRPWHPQGD
                     LTVSGSAVFLNCWMDDHIGPEGYAAIGSTDSSGKKLSFEIEPDSRFFEFGSSGPGAPA
                     SPARPRLTAEAAAWYTRDQLLRGWKP"
BASE COUNT         1764 a         2379 c         2169 g         1827 t
ORIGIN      
        1 tgatacgcag ggtgccgttg acctccgcaa gcgtgaaggt ggtgctgttt ggggcctccc
       61 agccggtgag caaattgtcg ttgaaatcct cccgcacgcc ggtgacctgg gcgggcagca
      121 gggcagcgga taaaagaatg aaacagaaca caaggatgaa agcacttttt tgcatgtttg
      181 cctcagttta tagagattgg cattggataa ttcaagggag tgtaggcctg gccggcgaac
      241 aagcgttgcc gatagaccaa caaactggcc ggtcgttctg ctgcatcggc gcctcattga
      301 aagggcgcgt cggttactca agcgcgcccc tttgacattg gattagtgca aatcccgggt
      361 cctatttgcc caaagccgcc atttttttgg catacttgcg caggatcttg agcgtgctct
      421 tgtcgctgtt gaaaaccgaa ttgcggccct gcggctcctg ggggggatcg ccggtatagg
      481 gatcgccctt gcgccaggta taggtttcag aatcagccgc tctgccttcg ccgccccatc
      541 cccaaaagtt gctcccggcc atgggtgagc ccgctttggc gctcttgtac atctcttcaa
      601 aaagcagttt gtagtagcga tcccgccagg ttaccccagc tgttcttgca taggcatgtc
      661 cgtcgcgcgg aaggccgaac tcctcgaggg tgaccggctt gcccagcttt tcggcgtagg
      721 cgatgtgttg gcggacataa tccagggcct tacgcgccgc atcgggccag atctcaccgg
      781 gcttgagcgg atcgaaccag gaccagttga gcagccagag gtggaaggtg aggtaatcaa
      841 tatgggggct ctggtgtgac tccaggtagc agctctccga ctccaggctt cctttcagtc
      901 cttcgttgcc ggtggtgacc aaatggttgg ggtcgaggga tttgatatag cccgcagtct
      961 cgtccaccca gaccgcaaaa tcggcgagat gctgtgcacg gttctcctcc tcaggagagg
     1021 ggcgcggttc attggccagc tgccaggcca tgatggtcgg atcctccttg taccagcggc
     1081 cgttgaccga gttgacacgg ttgaccagca tgcggatata gtcccgatag atgggcttag
     1141 cccggtcgca ggtgtaaaag cgcccgcttg tccgcatcag accccagtag ccgaatttgg
     1201 gatcctgagg gctcggataa ttctccccgg tcacccagga catatactgg gacatcccgc
     1261 cggtccactc ccagtaatta ttgagaaaaa tcaccgcata catgccgcgt ttggccatct
     1321 cagcgaggag aaaatcgagt cccaccagca gtttttgatc gtataccccc gcttcgggct
     1381 ggatggcggg ctggatctgc cccgcgtagc cgagcccctc ggaggccccc atcacgcgca
     1441 gattatcgat cccgagtttt ttcagctcat ccagttcgcg cagcagccgg gcgcggtcgc
     1501 ccgcctcccc ctcctgcccg agcatgcagc cgtaccagag gttggtgcca aggaaatagt
     1561 aggccttacc cttcaggtag aattgggtcc ccttaacctt gacgaaatcg gcgggctgag
     1621 cccgcagtgc aaccgataac gtcaccagcc aaatcaacac aacaaagcgc ttcataatcc
     1681 ttcctttgct tctgaatggg caagtacaag tcctgccaaa attgatactt ctggtatcac
     1741 cgttatttga ccaacaacat ttttttctgt tcaatgaatc tgccggcttt aagcctgtaa
     1801 aaatagaggc cactggcaag ttcgcgcccc acttcgggta gattgaaatt agctgtatgc
     1861 tctcccgcca gctgcagttt gttcaccaga acggccacct cccgtcccag cagatcgtac
     1921 actgtcaaag taaccatgac agggctggcc agataatagg tgatccgggt ggatggattg
     1981 aagggatttg gatggttctg atctagcgca cattcctgtg gcattgacgt aaagctttgt
     2041 tctgccaggg tgggattctg tttgtattta agggtggtat atgcagaggt tccgaatgag
     2101 ttatcgctgg ttccggtaac ataaacggtt ccggtcgcat ccaccgcaat atcagaaccg
     2161 gagtcattgt atcccgggag gtcaaaaagc aacgtccact gctcatttcc tgctgcatca
     2221 tatttgatcg tagcatattc ataatagtta tttgacccta cgcaagtgcc tgtgatatat
     2281 acattttcca ggtcatccac aacgagtgca ttagcgtaat cgtaggaatt tcgtgcccca
     2341 ttgtagcgcg ctacccattg ctcatatcca gccgcgtcgt atttaatcgt agtataatca
     2401 taggaggtgt aagcgccgcc actgtatccc gtcacataca cattgcctga ggcatctact
     2461 gcaagagcat tggcagcatc ataagaactg agcggtccgt catagcgcat cacccatagc
     2521 tcactacccc ctgcatcata tttgatcgtt gttatatcat aagatgtacc cgaacccgta
     2581 ctataaccgg ttatgtagat attccctgct gtatcgacag caagatcaaa ggcctcatca
     2641 tctgcatggc ctggcccatt gtagctggca acccactgct caacaccaga agagttgtat
     2701 ttaatcgtcg tgatatcaaa ggtggtgtct gagcctgttc catgcccggt cacatagaca
     2761 aaccccaggt catcaacggc aatagcgtta gccacatcgt cgtccttgcc aggcccgtta
     2821 tagcgtgcca cccattgttc cgctccagcc gcgtcatatt tgatcgtagc ataatcatag
     2881 gaggtgtctg cgccaacgct gtatcctgtc acatagacat tgcccgaacc atccagcgca
     2941 agggcgttga ctccatcata agagttaaac ggcccgttat aacgcgcgag ccacacctga
     3001 tctccattct cactgtattt tatcgtggca aaatcattcg aattctcata atccacgcta
     3061 tagcctgcca cataaacatt gcccgatcca tcaacaacga gggcgttagg ctcatcatct
     3121 gaagaggagg atccgtcgaa gcgcatcatc cacttttcag agcctgctgc atcatatttc
     3181 accgtgacaa tatccttata cgtatctgag ctcagactat ggccggttac gataacattt
     3241 cccgaattat ccacagcaag tgctctggct tcatcatcag aattgcccag gccattatag
     3301 cgcaccgcca actgctcatt tccatccggg ctgtatttta ccgtggcata gtcataatag
     3361 gtatcgagac cggtgctgta ccccgttaaa tagatattgc cggattcatc agcagcgaaa
     3421 gcacctggct gatcatcaga atatcccggt ccattatagc gcgccaccca ttgttcattc
     3481 cctgctgcat cgtatttgat cgtggtgtag tccttggacg tttgaaaacc aatgctggaa
     3541 ccggccacgt aggcattccc cgagccatcc aggtagataa agctggcgta atcagcagaa
     3601 ttgcccggac catcgtagct caccgcccac ttctcatttc ctgcctgatc gtatttgatt
     3661 gtagcaaaat ccagatagga tgttgaatct tcgcaaaacc cggtcacaaa aacatttcct
     3721 gatccatcaa ccgccagggc atacccccca tcatgcgagt tacccggccc attgtaacgc
     3781 gccacccact gttgaactcc agcagcatca tatttgactg ttgcataatc actatatgtg
     3841 cccaatccgt tgcttgatcc tgtcacatag acatttcccg atttatcaac agccagggca
     3901 ttagcctcat cgtcagaaga gagtggaccg tcgtagcgcg aaacccaaac ctgcactcct
     3961 gctgcatcgt acttgatcgt agtataatca ttggaagtcg ctgagccgcc gctgtaccct
     4021 gttacataga cattccccgt tccatcgacc acgagggcac ttgcgccgtc ataagaatta
     4081 agcgcgccgt tataacgcgc cacccagagc tgatcgccat cggctgtata cttgattgtt
     4141 gcgtaatcat tgtaactgtc tgaacctgtg ctgtacccgg taatatagac atatcctgag
     4201 gaatcaactg aaagagcgct gggctgatca tcagaatcgc ccggcccgtt gtaacgcctc
     4261 acccattttt ctcttccatc agcatcgtat ttgatcgtaa taaagtccca ggaagaggat
     4321 ggactcgcgc tgtatccggt cacatagaca ttccccgtgc catccacggc aacggcaacc
     4381 gaatggtcct catagccact atcatatcgg gcaacccaga tttccgtgcc tgaagcatca
     4441 tatttaacag tgcagtaatc cgcgccaaag gataattggg tgctgcggcc tgttacaaaa
     4501 aaattgcctg acttgtcgac agcgatggca gtcgcctcat cgatggattc attagctccg
     4561 gatccatagt gcttcgccca ttccagggtc gccgtacctg caacagcatt ttgcagcctg
     4621 ctgttcatca aaccactctg cagattgctg ctccgcggat atgcactctt cgcctcccgc
     4681 catttcccgt ctggcagcaa aggcggtttt tttcgagtgg gaatctcccg gatagatcct
     4741 tttacactca cctgaccaga aagaatgata tgcattggaa aaagtgcgat caatgcaatc
     4801 ataagcgtcg taagcagtac aagctttctc atcttatcct cctgtcatca tcaaatctgg
     4861 agcaacctgc catttgtccg cagactgcgc ctcttttcat cagcgccaga taaaaaggag
     4921 aatatcccca ctgatgccgg ctgctgcacc gcctccgcag attgatcgtg cattcggtgg
     4981 agtgttagtg acttttgcat cagagtcatt tgatcaacat catccgtcgt tgccgtctct
     5041 ccaggccagc ggtcaattga taaaagtaga ggccactact gaagccggat gcatccacaa
     5101 taatctcgtg ccggccttcc gcctgtatcc cgggatcaag ggtgcggaca gcgcgcccgc
     5161 tgacgtcata aatcaccagc cggattgggg ccggccgggt gagcctgtag gcgatggtcg
     5221 tgaccgggtt gaaggggttg ggatagttct gttcgagttc aaagagggcg ggcactccgt
     5281 cgtgccggtc cacgccgacc ggcatggtcg tgccatagag ttcgaattcg gtgacagagt
     5341 aattggtccc gctgactttc tgacagagca ggcgcacata gcgcgcctgt gtgtcaatat
     5401 caataatctc gtccaggccg ccgtcgccat ttctctgggt tatggccttg atggccgttc
     5461 catcggcatt gagtagatga acagtatagc tgactgcaaa gctgtttccc cagtggatcc
     5521 gcagggtctt gagcgtgatc atccggccaa gatcaaccat aatccattct gtgatcttgc
     5581 cggcgctctg ccaggcggtg gtggtatcgc cgtccaccgc ccattgggcg ttgccgccac
     5641 gcagggttga ggaggccgtc aggggatggc gcagggcgcg attgatgacg gccgcgggga
     5701 gcacctcgat ggtgatccgg gcgctttgac tatccccccc gccggcggcg agggtgtagg
     5761 tggtcgtccg ggcgggcacc accgccacgg agccgttctg cgccacagcc gcgccatcaa
     5821 gggtgaccga ggagccgttg gtggtcatcc aggagatgcg gctggtgtcg cccgcccctt
     5881 catcaagctg gagggggaaa gcgctcaagg acttgatgcg gccgggggga atattgtcga
     5941 tggccacccg cttggtgtcg gcgaccgccc cgctggcgat cagggtataa atgctcttcg
     6001 cggcgggagc aacgaccagg gagtcggagg cgccgaccgg cgcgccgttg agggtcacct
     6061 gagagccggg cgtggcctcc cagtagagct ggctggtctc gcccacctcc acctcggtgt
     6121 cggtagcggc gaagaggcgg atgaagccgg aggggctgtt gttgagatac caggccgcag
     6181 tatcagccat catggcataa ccggtggtgt tgggatggat gccatcgggg aagaggtcac
     6241 catggctgag catcgcgcga taaaaatcga tcataaagcc gccggcggcg acgcgaacgg
     6301 aatcgacgat cggttcgatc tgcccgtgca gcaccgcacc gttgatgccg ctgccaccgc
     6361 catcgccaaa gaccggacag ggtcgggcaa catagatctg gggatcgcgg ccatccttgc
     6421 gcagcacctt aataaaatcc atatagtcag tgaaaaattc accgagaaga acccggttct
     6481 ggggcttgct gtcgttcgtg cccaggcaga tgatgacaat atggggatca aaatcctggg
     6541 cctgcaggaa agccgattcg ttccaatagg ggaaatcccc atgccgcagc agggtggtgc
     6601 cgctgacccc gaaattgcgc acattatagt gcgcgcccag cctctgcccg agctgctgag
     6661 gccaggcggt agagccgccg ctgccgatgg tgatgctgtt gccgatgcag gcgacgcgga
     6721 tgacccggtc ctggccggaa agtgtcgtca cccaaagggc tgcgagcaga acggtggaga
     6781 ggattttttt catgcgcttc tccctccatg aaaaaagttt gacttcgact taaatttttc
     6841 gtaaggtaaa tcaagttttt ttttatcaga aaggacaacg atgcacaaga gcctttgttt
     6901 gctgctgccc ctacttttcg cttcggcctg cacccgaccg gccgcctctt cgtgggatgc
     6961 ggtcgtcgac gccggttatc gggggaaacc aggggcgaaa ttggcgggcg tgccctgtta
     7021 cccctctctg agccaggcgc tcgccgccgc gccgccaggt gaaaccccct ggcgcatatt
     7081 catgcgtaaa ggccgctatc atgaaaaggt gacaatcgac cgcgccaacc tgcatctgcg
     7141 cggcgagaac cgggaagagg ctatcctctc cttcgatgac tgcagcggca cccccgctcc
     7201 gggtggctcc accctgggca cccgggggag cgccaccctc actatccggg cccccggctt
     7261 ctcggcggag cacatcacca tcgagaactg cttcgactat ccagccaatg ccctgcgcgc
     7321 cgaaaacgac cccgccaaaa tcgcccaccc ccaggccgtg gcggtgatga tcagcgccgg
     7381 tggtgacagg atcagctttg ccgactgcgt catcagcgga tttcaggaca ccctcttccc
     7441 cgatgccggg cgctccctgt tcagcagatg ctgcatcctc ggccatgtcg attttatctt
     7501 cggggccggg caggcgctct ttgaacagtg tgagatcgtc tcgcgcgacc gcccgggcaa
     7561 gaatccaagc ggatatatca ccgcaccaag cacttcagca gcctatccct atggctttct
     7621 tttcatcgcc tgccggttgc tcaagcaggg gcccggagtg acggcgggat cggtccgcct
     7681 cggccgcccc tggcatccgc agggggatct cacggtatcg ggcagcgcgg tattccttaa
     7741 ctgctggatg gacgaccata tcggcccgga gggctatgcc gccatcggct caaccgactc
     7801 gagcgggaaa aaactttctt tcgaaattga accggattcg cgctttttcg agttcggcag
     7861 cagcggcccc ggcgccccag cctcgcccgc ccggccgcgc ctcacagcgg aggctgcagc
     7921 ctggtacacc cgcgatcagc tcctgcgcgg ctggaaaccc tgacatcccc cgcgcctcag
     7981 ccggcctctc agcgggcttc tggcccgcag ccactgccag ggtcaaaacg ccctctagcg
     8041 cagcagcagg cacttctgga ttgcccgctc accctccgcc tccaggacca gaaaataggc
     8101 gcctgaaggc cggcccgccg tctgccagac cagacggtg
//
LOCUS       DAOPWU010000146         8074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8917_length_8074_cov_4.705325, whole genome shotgun sequence.
ACCESSION   DAOPWU010000146 DAOPWU010000000
VERSION     DAOPWU010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8917_length_8074_cov_4.705325"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PKM23_08670"
     CDS             <1..990
                     /locus_tag="PKM23_08670"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNY91586.1"
                     /translation="GAVRALQPRKEATTVVLHPEVESAVQPIPRPEKSALAARIGRKF
                     VLSVEVLSPRGISVEGVLAGVRKLAGFGVDAINIPDGPRASSRMSPSALALLIERSLP
                     VETILHYCCRDRNLLGMQSDLLGHYALGLRNILIITGDPPKLGDYPSATAVFDVDSIG
                     LTRMVNGLNHGHDLAGNPIGAPTGWHIGVGANPGALDLDLEIARLEQKVAAGAEYLLT
                     QPVFDPHQLESFFKRTEHLRIPVLAGIMPVVSHRTVEYLNNEVPGVVIPEEIVRRFAG
                     AADSAHARETGIEIARKGILELRQFEQVSGLYVMPPFTKDKYDVAIEVLQVVQ"
     gene            1001..2257
                     /locus_tag="PKM23_08675"
     CDS             1001..2257
                     /locus_tag="PKM23_08675"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNY91587.1"
                     /translation="MNKIRMLLIFALLIALTPAFSQPGPGRNRNAFGADLFQIRFFRS
                     FIPEKKPEASVDSYIQMANDVLTFIKTDSTSWRARFEVELLIYDARGDLAAYRTLRDT
                     ITVGRFELTNARGISWTQAFRFYLKPGGYTWRLKLLNAEGIALLEREERLEVAELNPD
                     RLQISDIILADSLDCETGLYAPNLRGAFRRQNSAIGVIFELYPPAGSDSVRVWLTLMD
                     AARREMVSKSLVRPAASILRYCIDLADEITRPGDYTVQLSARSGSRLIQAAQRVLVLW
                     GQAEVQQESLDLAVEQLALIARGSTIREMERAQGALRDSLYEAFWQKRDPTPETPLNE
                     LREEFFRRIDHSNRSFSESFSGRQGWRTDRGRVYIQNGPPDQIEKHAGEMGMASTEIW
                     AYNRLNRRYLFVDRLSNGEFRLAKTE"
     gene            2345..2968
                     /locus_tag="PKM23_08680"
     CDS             2345..2968
                     /locus_tag="PKM23_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91588.1"
                     /translation="MKLWQRGSLETDFGPLLLIWRHRAAGRFYSKIFSWLLGGFVLGL
                     LASITFNAIGLGGFSLSAGRITFFVVFILGVVSAFFRNLAYGLDYRITQKALLHVRPL
                     CGIERLRPAGGKLRKGDRIDYLPWEEIKSVNEAGGALALTLKEGQVVELGVVPVLALW
                     LPTPDGGMEKRTSAQGGGKSSAELDKEAHKLIVQKIRDLKKNTTAKN"
     gene            3056..3853
                     /locus_tag="PKM23_08685"
     CDS             3056..3853
                     /locus_tag="PKM23_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91589.1"
                     /translation="MPSRITPLDVSAYATPEAFRTGEQIYENGWVRHRFQTNYGLSAM
                     VRGKGNYRVEMIIDGEQFFGRCTCPVGSSRCEHQVALLLSWLNEPQTFTSYQELRKAI
                     RLREKNALVDIFVNLIEVFPELSSFFISPSGRDELAAIQEDVAEVFDFPQAQKIDPQQ
                     IVESCQFLFVRAKSLRNEGKYAHARVIFFQILNRSLALIDRLQTIRPFRENFIAEIAD
                     DYEDLATSDPDFAASREAIAGEVEQILAHESAVAEGVFLDLVRQKLG"
     gene            3987..4706
                     /locus_tag="PKM23_08690"
     CDS             3987..4706
                     /locus_tag="PKM23_08690"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNY91590.1"
                     /translation="MKKSIRTLAVILPVFLFMSAARADVFMKEKEHTDAATVMGQTMP
                     ASEKIKSIWMTADKVRSDEPEASVIMRMDKGLLYILQHAQKSYMEVPLSFTAAAQAPA
                     ADLGIKVTVTPTAEKKKIGTWECKRYLMKMEMGGMGFATESEIWATEEIKIDPALYAK
                     FSSAMMAANPMLASSLQSMLEEMKKIKGVQVRSATSVQMMGQTIKSTSELIEFREGKA
                     PEGIFELPEGYNKKSMMDDLN"
     gene            4703..5506
                     /gene="dapF"
                     /locus_tag="PKM23_08695"
     CDS             4703..5506
                     /gene="dapF"
                     /locus_tag="PKM23_08695"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNY91591.1"
                     /translation="MKLSFVKISATGNDFILVDNRSGIWSAREDASFFSAICQHRRSV
                     GADGVILLEESSRADFHYIHVNSDGSLAGMCGNGSRAVAWFARQLGIGGDELSFEISG
                     SIYRARVEGRHVTTAFIPPGGFDLGLNVAGDAMELSAGGYIDTGVPHYVLFSTEVASV
                     DVAGRGAWYRRHPAFPQGANIDFVQVLDSHHLRVRTYERGVEAETLACGTGAVASSVI
                     SHLRGRASSPVEVDFPGGQLVVVFNPDYSGITLAGTVDPVYAGELWDES"
     gene            complement(5578..6996)
                     /locus_tag="PKM23_08700"
     CDS             complement(5578..6996)
                     /locus_tag="PKM23_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY91592.1"
                     /translation="MKGSDLLQFSLDNLRRTRLRTFLTTLGVIIGIGALVAMVSFGTG
                     MQRNITEAFTANDLFTTIRVLPVRMDIEQVMAGNLDGAMSGMQKRTRALNDTAIALIA
                     AMPEVAVVFPEITFPVKIRFGGQETSTTLRSVPVAMGGYTPYDRMGWGGFFSSDSAAE
                     VILSYGLLSRLKLTLDGEDEQARTDSSRVWRRVKADSLIGAEVEVITSVLDLGRMMSG
                     GLAAAGQPSFTEHVTRLRVAGIRPRPHAFSNEQVQGGIVVPLLTGKRMPRFAFNNMWS
                     LLSRGGRPTGSYEALYVRLNHLQDLPAIRKRLQELNFSVFALADQLEEIKRGFLMVDT
                     ALGAVGTIALVVAALGIINTMVMSILERTREIGVMKAVGASERQIRTIFFFEAGCIGL
                     MGGMLGVLLGWLVSRLANWIANLWTLRQGGGVGGDIEFFYLPPWLIGGAILFAVGVSL
                     AAGYYPATRAARVNPVEALRHD"
     gene            complement(6993..7694)
                     /locus_tag="PKM23_08705"
     CDS             complement(6993..7694)
                     /locus_tag="PKM23_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY91593.1"
                     /translation="MSALVEAIDLHKHYQKGKAEVAALRGVSFAVEAGEFISLVGRSG
                     SGKSTLLNIIGGLDTPSSGELRVGGRNLAAMDRELLARHRRTTVGMIFQSFNLIPSRT
                     ALENVALPMIFAGMERPVRSRRAAELLDLVGLDQRHTHKPAELSGGEAQRVAIARALA
                     NDPAILLADEPTGNLDSHTAAGILELLARLNQERALTILMVTHDQPSAERLSDRLLHM
                     LDGGIIGEERGGRRA"
     gene            complement(7841..>8074)
                     /locus_tag="PKM23_08710"
     CDS             complement(7841..>8074)
                     /locus_tag="PKM23_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HNY91594.1"
                     /translation="AKVAEALQSNCGVYLVSVGGAAALIAKSIKASEIVAWPELGAEA
                     IAKLTVVDFPAVVAQDCHGGNIYQEGIARYGIK"
BASE COUNT         1568 a         2613 c         2454 g         1439 t
ORIGIN      
        1 ggcgccgtcc gcgccttgca gccgcgcaag gaggcgacca cagtggtcct ccaccctgaa
       61 gtggagagcg ctgtccagcc catccctcgc ccggaaaaat cggccctggc cgcgcgcatc
      121 ggccgcaagt ttgtcctttc cgtcgaggtg ctctccccgc gcggcatctc ggtcgagggg
      181 gtgctggcgg gggtgcgcaa gctggccggc tttggggtcg atgccatcaa catccccgac
      241 ggcccacgcg cctcatcgcg catgtcgccc agcgcccttg ccctcctcat tgagcgctcc
      301 ctgccggtcg agaccatcct ccactactgc tgccgcgacc gcaatctgct cggcatgcag
      361 tcggacctgc tcggccacta tgccttgggg ctgcgcaaca ttctcatcat caccggcgat
      421 cctccgaaac tgggcgacta tccgagcgcg accgcagtct ttgatgtcga ttcgatcggc
      481 ctcacccgca tggtcaacgg cctcaaccac ggccacgacc tggccggcaa ccccatcggc
      541 gcacccaccg gctggcatat cggggtcggc gccaaccccg gcgccctcga tctcgacctc
      601 gaaatcgccc gcctcgaaca gaaagtggcc gccggagccg aatacctgct cacccagccg
      661 gtcttcgatc cgcaccagct tgaaagcttc ttcaaacgga cggagcacct gcgcatcccc
      721 gtcctggccg ggatcatgcc tgtggtcagc caccgcactg tggagtatct caataacgag
      781 gtgccaggcg tggtcatccc cgaagagatc gtccgccgct tcgccggcgc cgccgacagc
      841 gcccacgccc gtgagaccgg catcgagatc gcccgcaagg ggatcctgga actccgccag
      901 ttcgaacagg tgagtggcct ctatgtgatg ccccccttca ccaaggacaa atatgatgtg
      961 gccattgagg tcttgcaggt tgtacaatag gatggcaaat atgaacaaga tccgcatgct
     1021 cctgattttc gcgctcctga tcgccctcac cccggccttc agccagcccg gccctggccg
     1081 caaccgcaac gccttcgggg ccgacctttt ccagatccgc ttctttcgct ccttcatccc
     1141 ggaaaaaaag ccagaggcca gcgtagactc ctacatccag atggccaacg atgtgctcac
     1201 cttcatcaag accgactcca ccagctggcg ggcccgtttt gaggtcgaac tgcttatcta
     1261 cgacgccaga ggtgatctgg ccgcctatcg caccctgcgc gacaccatca ccgtcggccg
     1321 ctttgagctg accaatgccc gcggcatctc ctggacgcag gcctttcgct tctatctcaa
     1381 acccggcggc tacacctggc gcctgaaact gctcaacgcc gaggggatcg ccctgctaga
     1441 gcgggaggag cggctggagg tggcggaatt gaatcccgac cgcctgcaga tcagcgacat
     1501 cattctggcc gacagcctcg actgcgaaac cggcctctac gccccgaatc tgcgcggggc
     1561 cttccggcga cagaacagcg ccatcggcgt gatcttcgag ctctatcccc cggcggggag
     1621 tgattcggtg cgcgtttggc tcaccctgat ggacgctgcc aggcgcgaga tggtcagcaa
     1681 gagcctggtg agaccggcgg cctcgattct ccgctactgc atcgacctgg ccgatgagat
     1741 caccaggccg ggcgattaca ccgtgcagct gagcgcacgc tcgggctcgc gcctcatcca
     1801 ggccgcacag cgggtgctgg tgctctgggg ccaagccgag gtgcagcagg agtccctcga
     1861 cctcgccgtc gaacagctcg ccctcatcgc ccggggcagc acgatcaggg agatggagcg
     1921 ggcacaggga gcgctgcgcg acagcctcta cgaggccttc tggcagaagc gggacccgac
     1981 cccggagacg ccgctgaacg aactcaggga ggagttcttc cgccgcatcg atcacagcaa
     2041 ccgcagtttc agcgaaagct tttcgggacg ccagggctgg cgcacggacc ggggcagggt
     2101 ctatatccag aacggcccgc ccgaccagat cgaaaaacat gcgggtgaga tgggcatggc
     2161 ctccactgaa atctgggcct ataaccggct caaccgccgc tatctcttcg tcgaccgcct
     2221 cagcaacggc gaattccgcc tggccaaaac cgagtaaacg gttataattt gcttgataaa
     2281 ttcgaccgct tttttgtata ttggggcgta accattcccg caccctgaaa ggagtcctga
     2341 ggcgatgaaa ctgtggcagc gcggcagctt ggaaaccgat ttcggccctc tattgctcat
     2401 ctggcggcat cgcgccgccg gccgttttta cagcaagatc ttcagctggc ttctcggcgg
     2461 atttgtcctg gggctgctgg cctccatcac tttcaacgcc atcggactgg gcggtttctc
     2521 cctctcagcg ggccgcatca ccttttttgt ggtctttatc ctcggcgtcg tcagcgcctt
     2581 tttccgcaat ctggcctacg gcctcgatta ccgcatcacc cagaaggccc ttctccacgt
     2641 ccggccgctt tgcggcatcg agaggctgcg tccggccggc ggaaaactgc gcaagggcga
     2701 ccgcatcgac tacctgccct gggaggagat caaatccgtc aatgaagccg gcggggctct
     2761 cgccctgacc ctcaaggagg ggcaggtggt cgaactcggg gttgtccccg tgctcgccct
     2821 ctggctcccg actcccgacg ggggcatgga gaaacgcacc agtgcgcagg ggggggggaa
     2881 gagcagtgcg gaactggaca aggaggcgca caaactcatc gtgcaaaaaa ttcgtgatct
     2941 gaaaaagaac accacggcca aaaactgata tcgtccgccg gaacggacgc cgggtctctg
     3001 acggcaccgt ccggaaatat cacatcgttc tccggaataa cgaagggact caagcatgcc
     3061 ctcgcgaatt acccctctgg atgtctcggc ttatgcgacg cctgaagcat tccgtacagg
     3121 cgagcagatc tacgaaaatg gctgggtgcg acaccgcttt cagaccaact atggtctctc
     3181 agcgatggtg cggggcaagg gcaactaccg ggtggagatg atcatcgatg gcgaacaatt
     3241 tttcggccgc tgcacctgcc cggtggggag cagccgctgc gagcaccagg tggcgctgct
     3301 cctcagctgg ctgaatgagc cccagacctt caccagctat caggaactgc gcaaagcgat
     3361 ccggctcagg gaaaaaaacg ctctggtgga tatattcgtc aacctcatcg aggtgtttcc
     3421 ggaactgagt tcctttttta tcagcccctc cggccgggat gagctggctg ccattcagga
     3481 ggatgtcgcc gaggtcttcg atttcccgca ggcgcaaaag atcgatcctc aacagatcgt
     3541 cgagtcctgc cagtttcttt tcgtccgcgc caaatccctg cgcaatgagg gcaaatatgc
     3601 ccatgcccgc gtgatatttt tccagatcct caaccgctcg ctggcgctga tcgaccggct
     3661 gcagaccatc agacccttcc gcgagaattt cattgccgag atcgccgacg actatgagga
     3721 tctcgccacc agcgatcccg atttcgcagc cagccgggag gccatcgccg gcgaggtgga
     3781 acagatcctc gcccacgaat ctgcggtggc cgaaggggtc tttctcgatc tggtcaggca
     3841 aaagctcggc tgatccgcaa cttttattcc attggcaccg tatagctatc atccccctct
     3901 cgcgcaggcg cgcgaactcc cctcacgcgg gggagcagat atccggcagc ccgtccaccc
     3961 cgtcatttcc tcaacaggag gtcccgatga aaaaatcgat ccgcaccctc gccgtcatct
     4021 tgccagtctt tctcttcatg agtgcagccc gggcggatgt cttcatgaag gagaaagagc
     4081 acactgatgc agccacggta atggggcaga ccatgcccgc gagtgagaag atcaagtcca
     4141 tctggatgac cgcagacaag gtgcgcagcg atgagccgga ggcctcggtg atcatgcgca
     4201 tggacaaagg tcttctctat atcctgcagc acgcccagaa gagctacatg gaggtgcccc
     4261 tcagcttcac cgccgcggcg caagccccgg ccgctgacct cgggatcaag gtgaccgtca
     4321 cccccacagc cgagaagaaa aagatcggca cctgggagtg caaacgctat ctcatgaaaa
     4381 tggagatggg gggtatgggc tttgcgaccg agagcgagat ctgggcaacc gaggagatca
     4441 aaatcgaccc ggccctctac gccaaattca gcagcgccat gatggccgcc aacccgatgc
     4501 tcgcctccag cttgcagagc atgctcgagg agatgaaaaa gatcaaggga gtacaggtcc
     4561 gttcggccac cagtgtgcag atgatgggac agacaatcaa atccacgagc gagctgatcg
     4621 agttcaggga gggcaaagcc ccggagggga tctttgagct gccggagggc tataacaaaa
     4681 aatcgatgat ggacgatctc aattgaaatt gagctttgtc aagatatcag cgaccgggaa
     4741 tgatttcatc ctggtcgaca accgcagcgg gatctggtcg gcccgggagg atgcctcctt
     4801 tttcagcgcc atctgccagc accgccgcag cgtcggagcg gacggcgtca tcctgctcga
     4861 ggagagcagc cgcgccgatt tccactatat ccatgtcaat tccgacggca gcctcgccgg
     4921 gatgtgcggc aacggcagcc gggcggttgc ctggttcgcc cgccagctcg gcatcggggg
     4981 ggatgaactc tctttcgaga tcagcggcag catttacagg gccagggtgg aggggcggca
     5041 cgtgaccaca gccttcatcc cgccgggtgg attcgacctc gggctgaatg tggccgggga
     5101 tgccatggag ctgagcgcag gggggtatat cgataccggc gtgccccact atgtcctctt
     5161 cagcacagag gtcgcgtccg tggatgtcgc cggccgcggg gcctggtacc gccgccatcc
     5221 cgccttcccg cagggggcca atatcgattt cgtccaggtc ctcgacagcc atcatctgcg
     5281 tgtccgcacc tatgaacggg gggtggaggc cgaaaccctc gcctgcggca ccggggcggt
     5341 cgcctcatcg gtgatcagcc atctgcgcgg ccgggcttcc tcaccagtgg aggtcgattt
     5401 ccccggcggg cagctggtgg tcgtcttcaa tcccgattac tcaggcatca ccctcgccgg
     5461 tacggtcgat ccggtctacg ccggggaact ctgggacgaa tcctgaaaca gccgcagcca
     5521 taataaaaaa gcccggtgct cgccagagac cgggcttttt ttatgccggg ccgatgctca
     5581 atcatggcgc agggcttcga ccggatttac acgggctgcg cgtgtggccg gataatagcc
     5641 cgccgccagg ctcaccccca cggcgaagag gatcgctccg ccgatcagcc aggggggaag
     5701 atagaagaac tcaatatcgc cgcccactcc tcctccctgc cgcagggtcc agagattggc
     5761 gatccagttg gccaggcgcg agaccagcca gccgaggagc accccgagca tcccccccat
     5821 caggccaatg caccccgcct cgaagaaaaa aatcgtcctg atctggcgct cgctcgcccc
     5881 caccgccttc atcaccccga tctcgcgcgt gcgttcgagg atcgacatca ccatggtatt
     5941 gatgatgccg agcgcggcga cgaccagagc gatggtgccc accgcgccga gggcggtgtc
     6001 gaccatcaga aacccccgct tgatctcctc gagctgatcg gccagggcaa agaccgaaaa
     6061 gttcagttcc tgcagccgct tgcgtatggc cgggagatcc tgcaaatggt tgagacgcac
     6121 gtagagggcc tcatagctgc cggtgggacg ccccccgcgg gagagcaggc tccacatgtt
     6181 gttgaaggcg aaacggggca tccgcttgcc ggtcagcagg ggcacgacga tgccgccctg
     6241 gacctgttca ttgctgaagg cgtgcgggcg gggccggatg cccgccaccc gcagccgggt
     6301 gacatgctcg gtgaaagagg gctgcccggc cgcggcgagc cccccgctca tcatccgccc
     6361 cagatcgaga acggaggtga taacctccac ttccgcgccg atgagtgaat cggccttcac
     6421 ccggcgccag acgcggctgc tgtccgtccg tgcctgctca tcctcaccgt cgagggtgag
     6481 tttgagacgg gagaggaggc cgtaagacag gataacctcg gccgccgaat cgctgctgaa
     6541 aaaacctccc cagcccatgc ggtcgtaggg cgtatacccc cccatggcca cggggacgct
     6601 gcgcagggtg gtgctggtct cctggccgcc gaaacggatt ttgaccggaa aggtgatctc
     6661 gggaaaaacg accgccacct caggcatggc ggcgatgagg gcgatggcgg tgtcattgag
     6721 cgcccgcgtc cgcttttgca tgccgctcat cgccccgtcc agatttccgg ccatcacctg
     6781 ctcgatatcc atccgcaccg ggagcacccg gatcgtggtg aagagatcat tggcggtgaa
     6841 ggcctcggtg atgttgcgct gcatgccggt tccgaaggag accatcgcca ccagggcgcc
     6901 gatgccgatg atcaccccga gggtggtgag aaaggtgcgc agcctggtgc gccgcaggtt
     6961 gtcgaggctg aattgcagca ggtcggagcc cttcatgccc tcctccctcc cctctcctcg
     7021 ccgatgatgc ccccgtcgag catgtggagc agacggtcgg agaggcgttc ggccgagggc
     7081 tggtcgtggg tgaccataag gatggtgagg gcacgctcct gattgaggcg cgccagcagc
     7141 tcgaggatgc cggcggcggt atggctgtca agattgccgg tgggctcgtc ggccaggagg
     7201 atggccgggt cgttggcgag ggcgcgggcg atggccacgc gctgggcctc gccgccggag
     7261 agctcggccg gcttgtgggt atggcgctgg tcgaggccga ccagatcgag cagctccgca
     7321 gcgcggcggc tgcgcacagg gcgctccatg ccggcgaaga tcatcggcag ggcgacattc
     7381 tcgagggcgg tgcgcgaggg gatgaggttg aaggactgaa aaatcatccc caccgtggtg
     7441 cggcggtggc gggccagcag ctcccggtcc atggccgcaa ggttgcgccc ccccacccgc
     7501 agctctcccg aactgggggt atccagcccc ccgataatgt tgagcagggt cgatttgccg
     7561 gagccggaac gcccaaccag ggagatgaat tcccccgcct cgacggcgaa cgaaaccccg
     7621 cgcagagccg ccacctcggc cttgcccttc tgatagtgct tgtggagatc gatcgcttca
     7681 accagtgcgg acaagagcgc ctccttttca gagggcatac gcataacaac ggcggcttgt
     7741 ttcacttccc cgatgcagct caggccgccg atcctgatcc ggataaaaag agggctgtct
     7801 gcaaaccgga cagccctgcc atggtgaaga gaccggttgc ctacttgatg ccgtaacggg
     7861 cgatcccttc ctgatagata ttgccgccgt gacagtcctg ggccacgacc gccgggaaat
     7921 cgaccacggt cagtttggcg atcgcctcgg ccccgagctc gggccaggcg acaatctcgc
     7981 tggccttgat cgatttggcg atgagggcgg ccgcgccgcc gaccgagacc agatagaccc
     8041 cgcagttcga ctgcagggct tcggcgacct tggc
//
LOCUS       DAOPWU010000147         8031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8980_length_8031_cov_5.188566, whole genome shotgun sequence.
ACCESSION   DAOPWU010000147 DAOPWU010000000
VERSION     DAOPWU010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8980_length_8031_cov_5.188566"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1699)
                     /locus_tag="PKM23_08715"
     CDS             complement(<1..1699)
                     /locus_tag="PKM23_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNY91595.1"
                     /translation="MNLTGLDWGIVAVVIVFIFASVYASRTFMRSVADYLSAGRTAGR
                     YLISVSQSIAALGAITVIANFEMNYQAGFPMTWWGFTMGVVVLFITISGWVVYRFRET
                     RAMTMAQFFEMRYSKNFRIFAGALGVMAGIINFGIFPAVEARFFIYFCGLPDTFSLLG
                     VEISTFAFTVFLLLAISLYFVFTGGQIAIIISDFIQGAFVNIVFVVIILLFFSLFSFD
                     QLFQGLQTAPADASMLNPFKTSQAKDFNFWFFLIGVFGAIYSVLSWQGTQGFNSSAKT
                     AHEAKMAGVLSNWRGIPQVIFLLFIPVAAYTLMHHPDFLAKAGHVKGVLGTVNDHVLE
                     SQLTVPLVLTQLLPRGIMGAFVAVMLMASISTLESYMHSWGSIFVQDVVMPIRKKPLT
                     PQQHIKYLRLAIAGVALFVFAFSNIFRQTQYILLFFAVTGAIYAGGSGAVIIGGLYWK
                     RGTAAAAWSAMLTGSFIAVSGTVIPSILKANPGLGDSLPWLAALGAINGQIFWFLAMA
                     SAILVYILVSLLGPRHEYNMDQLLHRGRYAVKEDAMAASSEPVKGWRMLGMNKLFTRG
                     "
     gene            complement(1904..8020)
                     /locus_tag="PKM23_08720"
     CDS             complement(1904..8020)
                     /locus_tag="PKM23_08720"
                     /inference="COORDINATES: protein motif:HMM:NF020698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY91596.1"
                     /translation="MNNRVGLATYQIYIPKAGSYRLWLRGYAPDGLQNSCFYTFGTSD
                     TVTFSFSTFVSWTWESPKGSYNLPKGFVQLTIKNREAGLQLDKLLLTTNAGYVPSGTG
                     SKTQRFSNITDVSGSAYTFISFGSTAGQVVVRASAPAVPNGTNLDFNLYADALMPLAL
                     QYASETILTGTAGQPLEKEFAVLLKDTYNNFCVGVPVEFEVTEGDGHFSRQSTIRVSS
                     NSEGVASARFTLGYGTTNIITTSLPDLPDLAPLTFQAIAGEGIPVSISTISGDAQIDT
                     VRQTLAAPLVVQVLDEKSRPVLSYPVTFKIVRGNGLLDNSSVTVTDSTDSGGKASVIW
                     TLGDSAGTDNNLVRIDAPLTGAPILFTATALPEAPTIMTIVSGENQTGFAGEELKAPL
                     VVKISDLYGNGRADQRVIFSVVAGSGTFAGAATDTVMTDEAGQAAAVFKASTRTGPHQ
                     IRAQAASGSGIQDRIFYANIQPPRATNLVPVSGNGQEEIILRTLPKPFQVKVTNPFGN
                     PVPNTTVSFRVVGGGGTFGGRDSVDVLSDPTGLAEATLTLGTLAGEGLQRVRISAPGL
                     PITPIEFTATAFAGAAASIQPVSDVLFREAAESSVTLTVVVKDAYGNTKSGHTVAFAL
                     AQGNGALSAASVRTNGGGLASVQYRMGTSSAVPNIISATSTYAGSAEQLTGSPITFTG
                     TVLAGEPHQLIQLTAATGLGAKINSTLPEPFRVQVSDIHGNPSPQSLIVTFKVISGEG
                     TFGDNRTELERVTDANGQAFALLRVGALAGVNNNVVEVTVNERPQILPVRFTASTFPG
                     DPDSLAYAGEQSWSGRVQDEYTAKVVIRDIMGNVIEGQPVLFMVTRGGGSIRSAASST
                     ARDTITIQTNNVGVAAAFWRVGSRPDTNIVMASSAYQHSPLRGSPLQFTAAAAAEDPV
                     TLLRVSAERDTGVVHQPLSRPIRVRVVDGLGNPVPNHPVLFTVKYPEIPALKGKLFTA
                     GLADTATSKSVSTDAGGYAAVHFLLNAQVELNVVKVESRFNNNHLVKSPMDFYITGNP
                     SPAKSLQLLSGTSMTGAAGSPVTIRVRTLNGLGQPAGNHPVQFSVTDNYSSVGSPGIK
                     WISVLTNPVTGEASVTWLLGGKTGSRINSLEVNAGGLIGSPVTVTADVTAGAPYPPAC
                     RLTATDSVAADNIATSRLTVTLKDSFNNAVSGRSILFSSLEQGLIFIQPDQATNALGQ
                     AAGTVRSNRSGQKRISARLGDSGAEIATAQVVFVAGPPRQMAAHSSSAITVNAGTLAR
                     DSLAVLVTDALNNPVADIPITFTVINGSGYLLDSRLAAFTTRSNGLGIASTRFVAGIK
                     PGEATVIQAASSHPQLAKVQVNYVITVVPGLPAQVKKYAGDGQTGQAGSELPLPLQVR
                     VIDKSGEPVANIAVRFTAQDSGAAINPAEPVLTDYRGLAGVRYQLGYRAGEPPQIIDA
                     QISGTTFTASFIAYATGQGPKQLKVVASALQKGTAGKPSPAPMQVAVVDDFGNGVANA
                     PVSFRLADNNGGSQVILDSVRTNLSGNAALQPLLPTRVGEYIYIATSPLLPGQSAAFY
                     CTVGPDAAYRLAKFAHDYQSMTAGRELIYPVIVRVSDQYDNPVPDVAVQFTTMPNSDN
                     GEPLTPTAISDNEGLAAGRWRLSSQPKTNSLMANRFGLRDSPVWFTASGVLNQFPEFK
                     ELPVSPLRIEYNRSFSMTIQAVDDDGDALRYSLKMDPPVANALFDSAASRTFTWKPTV
                     RQKGEYQINLRVEDGRGGFDIDSLLVSVVGDSAPVFTSTYPVERQIKITRPASQLFTC
                     AAIDYDNDPLTFSWYIDGVKKAAGPRFEFRSDEHGDGAHYIWVTAFDGIKEVRSELWE
                     IDVATSVELSSFTAAAEPWKGVVVSWSTSHEAGNLGFELMRSTAARGEYIRITRQLIP
                     SREDGRYHYQDSSAVAGERYYYMIEDLDRSGVRTLHGPVEVMLQLPATYALEQNFPNP
                     FNPETAVRFQLPRSERVQLIVFNPLGQQVRVLADGKLEAGYHAVNWDGRGERGERVSS
                     GIYYYRLVTPRFQAVKKMALVK"
BASE COUNT         1462 a         2275 c         2851 g         1443 t
ORIGIN      
        1 cgccgcgggt gaaaagcttg ttcatgccga gcatgcgcca gcccttgacc ggttcggagg
       61 aggcggccat ggcatcctcc ttgactgcat agcggccgcg gtggaggagc tggtccatgt
      121 tgtactcgtg cctcggcccg agcagggaga ccaggatgta gacgagaata gcgctggcca
      181 tggcgaggaa ccagaagatc tggccgttga tcgccccgag ggcggcgagc cagggcagcg
      241 aatccccgag gccgggattg gccttgagga tcgaggggat gaccgtgcca ctgacggcga
      301 tgaaggagcc ggtgagcatc gccgaccagg ccgctgccgc tgtgccgcgc ttccagtaga
      361 gtccgccgat gatgaccgcg cccgagccgc ctgcatagat ggctccggtg accgcgaaga
      421 agagcaggat gtactgggtc tggcggaaga tgttgctgaa ggcgaagacg aagagagcaa
      481 ccccggcgat ggcgaggcgc agatatttga tgtgctgctg cggggtgagg ggctttttgc
      541 ggatgggcat caccacatcc tggacgaaga tgctccccca tgagtgcatg taggactcga
      601 gggtgctgat cgaggccatc agcataaccg ccacaaaggc gcccataatg cccctgggca
      661 gcagctgggt gagcaccagt ggaacggtga gctggctctc aaggacatgg tcgttgacgg
      721 tgccaagcac tcccttgacg tggccggcct tggcgaggaa atcgggatgg tgcatcaggg
      781 tgtaggcggc caccgggatg aagagcagaa aaatcacctg ggggatgccg cgccagttgg
      841 agagcacccc ggccatcttg gcctcatggg cggtcttggc cgagctgtta aagccctggg
      901 ttccctgcca tgataggacg ctgtagatcg ccccgaacac cccgataaga aagaaccaga
      961 aattgaaatc cttggcctgg ctggtcttga agggattgag catcgaggcg tcggcgggag
     1021 cggtctgcag cccctgaaaa agctggtcga aggagaagag gctgaagaag agcaggatga
     1081 tgaccacgaa gacgatgttg acaaaggctc cctggatgaa atcggagatg atgatggcga
     1141 tctggccgcc ggtgaagaca aagtagaggg agatggccag gaggagaaag acagtgaagg
     1201 cgaaggtcga gatctccact ccgagcaggg agaaggtatc gggcaggccg cagaaataga
     1261 tgaaaaagcg cgcctcgacc gccggaaaga tgccgaaatt gatgatgcca gccatgacgc
     1321 cgagagcgcc ggcgaaaatg cgaaagtttt tgctataacg catctcgaag aactgggcca
     1381 tggtcatggc gcgggtctcg cggaagcggt agaccaccca gcccgagatg gtgatgaaga
     1441 ggaccacgac gcccatggtg aagccccacc aggtcatggg aaagccggcc tgatagttca
     1501 tctcaaagtt ggcgatgacg gtgatggccc cgagggcggc gatgctctgc gagacgctga
     1561 tgaggtagcg cccggcggtg cgtccggcgg agagatagtc ggcgacgctg cgcatgaagg
     1621 tgcggctggc gtagacgctg gcgaagatga aaacaatcac cacagcgacg atgccccagt
     1681 ccagcccggt tagattcatg ggatatcctt tctcgatagg gggagaggcg cagcccccgg
     1741 ccggctgcgc acgcggaata gctgcacatt aagttaggca aacgttggtt ttttgtcaag
     1801 aaaaaatatc aacaaaatgg ccgcaggtgt gcgtcggccc acgcgcttga ataaaaaaaa
     1861 ggccgcggca tccgccgcgg ccctttttca agaccatcac ctcctatttc accagggcca
     1921 tcttcttgac cgcctggaaa cggggggtca ccagccggta atagtagatc cccgaactca
     1981 cccgctcacc cctctcgccg cggccatccc aattcaccgc atgatagccc gcctccagct
     2041 tgccatcggc cagaacgcgc acctgctggc caaggggatt gaagacgatc agctgcaccc
     2101 gctccgatcg cggcagctgg aaccgcaccg cggtctcggg attgaaggga ttgggaaaat
     2161 tttgctccag ggcgtaggtg gcgggaagct ggagcatcac ctccaccggc ccgtgcaagg
     2221 tgcgcacacc gctgcggtcg agatcctcga tcatataata gtagcgttcg cccgccacgg
     2281 cgctgctgtc ctggtagtgg tagcgcccgt cctcccgact gggaatgagc tggcgggtga
     2341 tgcggatata ctccccgcgc gcggctgtgc tgcgcatgag ttcgaatccg aggttgccag
     2401 cctcgtggct ggtgctccag ctcaccacga cccccttcca gggttccgcg gcggcagtga
     2461 aagagctcaa ctccacactg gtcgcaacat cgatctccca cagctcggat ctcacctcct
     2521 tgatgccgtc gaaagcggtg acccagatat aatgagcgcc gtcgccgtgt tcgtcgctgc
     2581 ggaactcgaa gcgcggaccg gcggcttttt tgacgccgtc gatataccag ctgaaggtga
     2641 gcggatcgtt gtcgtaatcg atcgccgcgc aggtgaaaag ctggctggcg ggcctggtga
     2701 tcttgatctg ccgttcgacg ggataggtcg aggtgaagac cggggccgaa tcgcccacca
     2761 cacttaccag cagggaatcg atgtcaaatc cgccgcgtcc atcctcgacg cggaggttga
     2821 tctggtactc tcccttctgg cgaacggtcg gtttccaggt gaaggtccgg ctggcggccg
     2881 aatcaaagag ggcattcgcc accggcgggt ccattttcag gctgtagcgg agggcatccc
     2941 cgtcgtcgtc gacggcctga atggtcatgc taaagctgcg gttgtactcg atacgcaggg
     3001 gcgagacggg cagctcttta aattcgggga actggttgag cacgccggag gcggtgaacc
     3061 agaccggact gtcgcgcagg ccgaagcggt tggccatcag gctgttggtt ttgggctggg
     3121 aggagagccg ccagcggccg gccgccagtc cttcgttgtc gctgatggcc gtgggtgtca
     3181 ggggctcacc gttgtcgctg ttgggcatgg tcgtgaactg gacagccacg tccggcaccg
     3241 gattgtcgta ttgatcgctc acccggacga tcaccgggta gatgagctcg cgcccggcgg
     3301 tcatcgactg gtaatcatgg gcgaacttgg ccaggcgata ggccgcatcc ggcccgaccg
     3361 tgcagtaaaa agcggcggac tggcccggta gcagggggga ggtggcgatg tagatgtact
     3421 ccccgacccg ggtcggcagc agcggctgca gggcggcgtt gccgctgagg ttggtgcgca
     3481 ccgagtcgag gatgacctgg ctgcccccgt tgttgtcggc gaggcggaag ctgaccgggg
     3541 cgttggccac gccgttgccg aaatcgtcga cgacggccac ctgcatgggg gcgggcgagg
     3601 gtttgcctgc agtccctttc tggagagcgc tcgccaccac cttgagctgt ttgggaccct
     3661 ggccggtggc gtaggcgata aagctggcgg tgaaggtcgt gcccgagatc tgggcgtcga
     3721 tgatctgggg cggttccccg gctcggtagc cgagctggta gcgcacaccc gccagtccgc
     3781 gatagtcggt cagaaccggc tcggcgggat tgatagctgc gccgctgtcc tgggcagtaa
     3841 agcggaccgc gatgttggcc accggctcac ccgacttgtc gatcacccgc acctgcaggg
     3901 ggaggggcag ttcgctgcca gcctgaccgg tctggccgtc accggcatat tttttcacct
     3961 gggcgggcag gcccggcacc acggtgatga cataattgac ctgaaccttc gccagctggg
     4021 ggtggctgct ggcggcctgg atcacggtcg cttcgccggg cttgatcccc gccacaaagc
     4081 gtgtgctcgc gatgcccagt ccattcgaac gtgtggtgaa ggcggcgagg cgggagtcga
     4141 gcagatagcc gctgccgttg atgaccgtga aggtgatcgg gatgtcggct accggattgt
     4201 tcagggcgtc agtgaccaac accgccaggg agtcgcgcgc cagggttccg gcgttgaccg
     4261 tgatcgcgct gctggagtgg gcggccattt ggcggggagg cccggccaca aagaccacct
     4321 gggcggtggc gatctccgca ccgctgtcgc cgagcctggc gctgattctc ttctggccgg
     4381 agcggttcga ccgcaccgtg ccggccgcct ggccgagggc gttggtggcc tggtcgggct
     4441 ggatgaagat cagtccctgc tccagggagg agaagaggat cgaccggccg gagacggcgt
     4501 tgttgaagct gtccttcagg gtgacagtca gccggctggt ggcgatgttg tcggcggcca
     4561 cggaatcggt cgccgtcagc cggcaggcgg gcgggtaggg cgcgcctgcg gtaacatcgg
     4621 cagtgacggt gaccggggag ccgatcaagc cgccggcgtt gacctcaagg gaattgatgc
     4681 ggctgccggt ttttccgccg agcagccagg tgacggaggc ctcgccggtg accgggttgg
     4741 tcaacacgct gatccacttg atcccgggcg acccgacgct gctgtagttg tcggtgaccg
     4801 agaactggac gggatggttg ccggccggtt ggccgagtcc gttgagggtc cgcacccgga
     4861 tggtcaccgg gcttcccgcc gctcccgtca tcgaggtccc gctgagcagt tgcaggctct
     4921 tggccgggct gggattaccg gtgatgtaga aatccatcgg tgatttgacg agatggttat
     4981 tgttgaagcg ggattcgacc ttgacaacgt tgagttcgac ctgtgcgttg agcaggaaat
     5041 ggaccgcagc atagcccccg gcgtcggtgg atacgctttt gctggtggcg gtatccgcca
     5101 gtccggcggt gaagagcttg cccttcaggg cagggatctc gggatatttg acggtgaaga
     5161 gcacaggatg gttgggcacc ggattgccga gtccgtcgac cacccgcacc ctgatcgggc
     5221 gggagagggg ctgatggacc acgccggtgt ctctttcggc cgagaccctc agcagggtga
     5281 ccggatcctc cgccgccgcg gcggcggtga actgcagggg cgagccccgc agcgggctgt
     5341 gctgataggc cgagctggcc atgacaatgt tggtgtccgg cctgctgccc acccgccaga
     5401 aggcggccgc gaccccgaca ttgttggtct ggatggtgat ggtgtcgcgc gctgtcgagg
     5461 aggcggcgct gcggatcgaa cccccgcccc gcgtcaccat gaagaggaca ggctggccct
     5521 cgatcacatt gcccatgatg tccctgatca cgaccttggc ggtgtattca tcctggaccc
     5581 tcccgctcca gctctgttcg cccgcgtagg cgagcgaatc gggatccccg gggaaggtcg
     5641 aggcggtgaa gcggaccggc aggatctgcg gccgttcgtt gacggtgacc tccacgacgt
     5701 tgttgttgac tccggcgaga gcgccgacgc gcagcagggc gaaagcctgg ccgttcgcat
     5761 ccgtcacccg ctccagctcg gtgcggttgt cgccgaaggt gccctcaccg gagatgacct
     5821 tgaaggtgac gatcaggctc tgcgggctgg gattgccgtg aatgtcgctc acctgcaccc
     5881 ggaagggttc cggcagggtg ctgttgatct tggctccgag gccggtggcg gcggtgagct
     5941 ggatgagctg gtgcggctcg ccggcgagga cagtgccggt gaaggtgatg ggtgagccgg
     6001 tgagctgttc ggcgctgccg gcgtaggtgg aggtggcgga gatgatgttg ggcaccgccg
     6061 aactcgtccc catccggtac tgcacggagg cgaggccgcc gccgttggtc ctgaccgagg
     6121 ctgcgctcag ggcgccgttg ccctgggcga gggcaaaagc caccgtatgg cctgatttgg
     6181 tgttgccgta ggcatccttg actaccaccg tgagagtcac gctggattcg gccgcctccc
     6241 ggaagaggac atcggagacc ggctggatcg aggccgccgc cccggcgaag gcggtggcgg
     6301 tgaattcgat gggcgtgatc ggcaggcccg gagcgctgat gcggacgcgc tgcagcccct
     6361 ccccggcgag ggtgccgagg gtgagggtag cttcagccag accagtcggg tcggagagaa
     6421 catcaaccga gtcgcggccg ccgaaggtgc cgccgccgcc cacgacgcgg aagctcaccg
     6481 tggtgtttgg caccggattc ccgaagggat tggtcacctt gacctggaag ggcttgggca
     6541 gggttctgag gatgatctcc tcctggccgt tcccggagac cggcacgagg ttcgtcgctc
     6601 gcgggggctg gatgttcgca tagaaaattc tgtcctggat ccccgatccg gaagcggcct
     6661 gcgcccgaat ttgatgaggg cccgtccggg tggaggcctt gaaaacggcg gcggcctggc
     6721 cggcttcatc ggtcatcacc gtgtccgtcg ctgcgcccgc aaaggtgccg ctgccggcca
     6781 cgacggaaaa gatgacgcgc tggtccgcgc ggccgttgcc gtagaggtcg ctgatcttga
     6841 ccaccagggg agctttgagt tcctcgccag caaagccggt ctggttttct cccgagacga
     6901 tcgtcatgat cgtcggggcc tccggcaggg cggtggcggt gaagagaatg ggggccccgg
     6961 tgaggggggc atcgatgcgg acgaggttgt tgtccgtgcc ggccgaatcg cccagggtcc
     7021 agatgacgct ggccttgccg ccgctgtcgg tcgagtcggt gacggtaacg gagctgttgt
     7081 cgagcaggcc gttgccgcgc acgattttga atgtgaccgg atagctgagc accggccggc
     7141 tcttttcgtc gagaacctgc accaccaggg gggcggccag ggtctgcctg acggtgtcga
     7201 tctgggcgtc gccagaaatg gtgctgatgg agaccggaat gccctcgccg gcgatggcct
     7261 ggaaggtgag gggcgccaga tcggggaggt cgggcaggga ggtggtgatg atattggttg
     7321 taccatagcc cagggtgaag cgggcgctgg cgaccccctc gctgttcgag gagacccgga
     7381 tggtgctctg gcgcgagaag tggccgtcgc cctccgtgac ttcaaactcc accggcacgc
     7441 ctacacaaaa gttgttgtag gtatccttga gcagcaccgc gaactctttt tcgaggggct
     7501 gccccgctgt gccggtcagg atggtctcgg aggcgtactg cagggccagg ggcatgaggg
     7561 catcggcgta gagattgaaa tcgagattgg tgccgttggg aacggccggg gcggaggcgc
     7621 gcaccaccac ctggccggcg gtggagccga aggaaatgaa ggtataggcc gagcccgata
     7681 catcagtgat gttggaaaag cgctgggtct ttgaaccggt gcccgagggg acatagccgg
     7741 cattggtggt cagcagcagc ttgtccagct gcagtcccgc ctcgcggttt ttgatggtga
     7801 gctggacgaa gcccttgggc aggttgtagg agcccttcgg actctcccat gtccagctga
     7861 cgaaggtgga gaaggagaag gtgacggtgt cactggtgcc gaaggtgtaa aagcaggagt
     7921 tctgcaagcc gtccggagca tagccgcgca gccagaggcg gtagctgccg gccttgggga
     7981 tgtagatctg gtaggtggcc agaccgacgc ggttgttcac cggcagatag g
//
LOCUS       DAOPWU010000148         8024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_8987_length_8024_cov_5.098758, whole genome shotgun sequence.
ACCESSION   DAOPWU010000148 DAOPWU010000000
VERSION     DAOPWU010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8987_length_8024_cov_5.098758"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="PKM23_08725"
     CDS             <1..1659
                     /locus_tag="PKM23_08725"
                     /inference="COORDINATES: protein motif:HMM:NF037850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HNY91597.1"
                     /translation="PSVILWSIGNEEYVIQGNETGAAIASTMMARLRRLDPGRTITYG
                     ANIGPDAGGINTVIPVRGFNYKRNCAIDRYRSLRPDQPLIGSEEGSTVCTRGFYANDT
                     LKGYVCDYDSMYPPWATTAQDWWSFYADRPWLSGGFVWTGFDYRGEPTPYSWPCINSH
                     FGIMDMCGFPKNNYFYYQAWWSGKDVLHLFPHWNWPGREGEEIEVWVHSNCDSVELFL
                     NGRSLGRMSMPRNGHLAWLVPYESGRLEAAGWRSGRRLAAAVETTGPAKGIHLAPDKS
                     SIRADQEDLCMVTVTARDGAGREMAVADQLIHFELQGPGRIIGVANGDPSSHEADKCA
                     DGSWQRRLFSGKCQVIIQALRESGEILLTASAPGLLAHTVAIRAESCTPRPLVPPYQP
                     DQLRHLGLGMPVHYGKSFSYKWPGAGAKTLSNGLLGSSEYNDGQWQGFEGEDLEAVID
                     LGAVQKIGSLCATFLQDAGSWIFWPKTVSFALSRDGRSFTPAGGVTACEDVRSSMIKA
                     FRVEAGGKRARYVRVTGVSVKVCPPDHRAAGSPAWLFVDEVVIE"
     gene            1670..4132
                     /locus_tag="PKM23_08730"
     CDS             1670..4132
                     /locus_tag="PKM23_08730"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HNY91598.1"
                     /translation="MTLSCLRKSWLVLILISAAGGADLQLWYDRPAEHFTESLPLGNG
                     RIGSMVFGGVDSEKLVLNEISLWSGAPQEADRPDAAAWLPQIRELLLRGENRAAQELV
                     MQHFVCAGAGSGYGSGANVPYGCYQTLGALRIAFTAADDEPVTGYRRELDIDEAIARV
                     EYRRGGARFTREYFTSAADQVLAIRLTASRKGALHFTLALDRPERFEMRTAGGNELIM
                     TGQLPDGSGGGGMRYAARLRVARCDGRVEAVGGQLTLSGASEVLLLLSAATDYSGFAG
                     RHTPDPEAAARQDLERACARDYAGLKARHIADYQSFFRRVALRLEPRSPAEKMAAQQP
                     TDRRLTNLQQGGSDPALMALYFQYGRYLLISSSRPGGLPANLQGLWAEEIQTPWNGDY
                     HININLQMNYWPAEVGNLAELHTPMLDYICALQEPGRRTARAYYNARGWVAHVISNPW
                     LFTSPGEGAAWGATVSGAAWLCHHLWEHYAFSGDRRVLEKIYPVLKESALFYSDLLIR
                     EPQHGWLVTAPSNSPENSYRTRDGFSGQICMGPAIDQQLLRALFGSCIAAAESLGVDA
                     GFSAALDSIRRELAPNQIGSQGQLLEWLEEYEEPEPHHRHVSHLWGLHPGDEISFEST
                     PELAAAARKSLERRGDDGTGWSLAWKINFQARLGEGDRALTLLRRLLQPNGARWAQGE
                     GGGSGTYPNLFCAHPPFQIDGNFGGAAGIAEMLLQSQGGMLRFLPALPGEWPEGEVTG
                     LRARGGFTVDIQWRQGRLKRARIHSANGAPCVIRWQGEPFKIRSSDGRPVRVSFEEGF
                     IRFACTKNSTTIITFKPSQRKS"
     gene            4129..7920
                     /locus_tag="PKM23_08735"
     CDS             4129..7920
                     /locus_tag="PKM23_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNY91599.1"
                     /translation="MRSIILLMLLALTLPAAALEMTAPAQEVEASVLRAARVTPSPRQ
                     YAWQQLETIAFAHFGINTFTDREWGEGSEDPGLFNPEAFDARQWARVCKEAGLKMIII
                     TAKHHDGFCLWPSAFTDHSVKKSPWRGGRGDVVKEVAEACREYGLKFGFYLSPWDRHE
                     ASYGDSLRYNAFFMNQLRELLTGYGEVSEVWFDGACGEGPNGKRQVYDFQAWYRLIRE
                     LQPNAVIAIMGPDVRWVGTEAGYGRTTEWSVLPAAAQNTDSISASSQQEASDRAFVPG
                     DLTGEDLGSREKIREAGRLVWYPAEADVSIRPGWFYHAEQDGQVKSPEKLVDIFYGSA
                     GRNCVLLLNIPPDRRGLIHENDAAALREMRRRLDATFQSNLLAGARLSADSGSDPCAA
                     ADGSLNTGWRAGDGRHSAILTADLGARKTFDVLALQEDILTGQRIEAFELQVEEGARW
                     RSVAYGTTVGYKRLLRFPPVTAARVQLVISRSRTEPALAEWGLYKQPPEEELRAAGGP
                     VGKKSDIPGLALKQEASPPLLLARRGGSSYRIVHPEKASAAEQRAAGLLQEYMRKSTG
                     AELPVVSETSPATEREILIGAVRRLPARLAQVQNQLSGDAFFIGHEGRKIIVLGGADR
                     GVVYGAVHFLEKYLGCRRYTPEVEVVPRHEELSLAVAEEIQAPANRFRSINTRFARDP
                     GYKEWLRLDEVQDIYARGWYVHTFDQLVPRAEYFADHPEYYCWMNGKRIHDQLCLSHP
                     EVLRITLDRLSKAMAEQPDKKYWSVSQNDNFSYCQCPACSQVIAEEGSPAGPVIRFVN
                     AVAAAFPDKVISTLAYQFSRQAPKITRPAENVHIVLCTIELNRSQPIESDPGSRDFVR
                     DIEEWSRLTKNLYIWDYVVQFTNFVSPFPNLHVLQPNLQFFVRNGGYEHFQQSNGQSG
                     GEMAELKNWLIAKWLWDPDLDADSLKEDFLRGYYGPAAPFIQEYAARLEAELLRSGDR
                     LDIYGSPVWHAGSVLSAENVADYNRMFDQAEAAVAGEEALLQRVRTARMPLQFAIMEI
                     GKNDLFGPRGWYREEGGSFLLRPEMRGLLEEFYAAGKSAGLRLVNESGFTLDDYYRTT
                     LRFIESRVEGNLAFRKTVSADPPPSPKYAGGDPGVLTNGVQGASDYKVHWLGWEGVDF
                     QVTLDLGSPASPARVRLATLYDPKSWILHPRRVTCLASADGFRFEPAGSLTVDGDQRQ
                     EEKVREFLFDALPAGVRWLRFEVEGTRHLPAWHPSEGGLSWVFIDEIVVESLAGTGEK
                     E"
BASE COUNT         1376 a         2668 c         2560 g         1420 t
ORIGIN      
        1 ccctcggtca tcctctggtc gatcggcaac gaggagtacg tcatccaggg caatgagacc
       61 ggcgcggcca tcgcctcgac gatgatggcc cgtctccggc gcctcgatcc cggccgcacc
      121 atcacctacg gcgccaatat cggtcccgat gccgggggga tcaacacggt catcccggtg
      181 cgcggcttta actacaagcg caactgcgcc atcgaccgct accgttcact ccgtcccgac
      241 cagcccctca tcggctccga ggagggaagc acggtctgca cccgcggctt ttacgccaac
      301 gacaccctca agggctacgt ctgcgactat gactcgatgt acccgccctg ggctacgacc
      361 gcgcaggact ggtggagttt ttacgcggac aggccctggc tgagcggggg attcgtctgg
      421 accgggttcg actaccgcgg cgagcccacc ccttacagct ggccctgcat caactcccac
      481 tttggcatca tggatatgtg cggctttccc aagaacaact atttttacta tcaggcctgg
      541 tggagcggaa aggatgtgct ccacctcttt ccgcactgga actggccggg ccgcgagggg
      601 gaggagatcg aggtttgggt gcacagcaac tgcgacagtg tcgagctttt tctcaacggc
      661 cgctccctcg gccggatgag catgcctcgc aacggccacc tcgcctggct ggttccctat
      721 gagtccggcc ggctcgaggc ggccggctgg cgctcgggcc ggcggttggc cgcagccgtc
      781 gagaccacag gaccggcgaa ggggatccac ctggcgccgg acaaaagctc gatccgcgcc
      841 gatcaggagg atctctgcat ggtgacggtc acggcgcgcg atggggcagg ccgcgagatg
      901 gcggtggctg atcagctcat ccatttcgaa ctgcagggtc cgggccggat catcggggtg
      961 gccaatggcg atccctccag ccatgaagcg gacaagtgcg cagacgggag ctggcagcgg
     1021 cgcctcttca gcggcaagtg ccaggtaatc atccaggcac tgcgtgagtc cggcgagatc
     1081 cttctcaccg cctctgcgcc gggcctgctc gcccacaccg tggccatccg ggcggagagc
     1141 tgcaccccac ggcctttggt gccgccctac cagcccgacc agttgcgcca cctcggtttg
     1201 ggcatgccgg tgcactatgg caagagtttt tcgtacaagt ggccgggggc cggtgccaaa
     1261 accctcagca acggcctgct cggcagcagc gaatacaacg acggccagtg gcagggcttt
     1321 gagggggaag acctcgaggc ggtgatcgat ctgggcgcgg tgcaaaagat cggctccctg
     1381 tgcgccactt tcctccagga tgcgggctca tggattttct ggcccaaaac ggtgagtttc
     1441 gccctctccc gcgacggccg ttcattcacc ccggccggcg gcgtgaccgc ctgcgaagat
     1501 gtgcgctcga gcatgatcaa ggcctttcgc gtggaggccg gggggaagcg ggcgcgctac
     1561 gtcagggtaa cgggggtcag cgtcaaggtc tgtccgccgg accaccgcgc cgccggcagt
     1621 ccggcctggc ttttcgtcga cgaggtggtg atagaataat ggaagacaaa tgactttatc
     1681 ttgcttaaga aaatcatggc tggtcctgat cctcatttcc gccgccggag gcgctgacct
     1741 gcagctctgg tatgaccggc cggcagagca ttttaccgaa tccctgcccc tcggcaacgg
     1801 ccgcatcggg tccatggtct ttggcggcgt ggattccgaa aaactcgttc tgaacgagat
     1861 ctcgctctgg tcgggggcgc cccaggaggc cgaccgtccg gatgccgcgg cctggctgcc
     1921 gcagatccgc gagctgctgc tcagggggga gaaccgcgcc gcccaggagc tggtcatgca
     1981 gcactttgtc tgcgccggcg ctggctccgg ctatggcagc ggggccaacg tcccttacgg
     2041 ctgctaccag actctcggcg ccctgcgcat cgcctttacc gctgcggacg acgaaccggt
     2101 gacggggtac cgccgggaac tggacatcga cgaggccatt gccagggtgg agtacaggcg
     2161 gggcggcgcc cgcttcaccc gggaatactt taccagcgcc gccgatcagg tgctggccat
     2221 ccgtctgacc gccagccgca agggggcgct ccattttact cttgccctcg accgtcctga
     2281 gcgttttgag atgcgtacgg ccggggggaa cgagctgatc atgaccggtc agctccccga
     2341 tggcagcggc ggcgggggca tgcgctacgc cgcccgcctg cgcgtggcgc gctgcgacgg
     2401 ccgtgtggaa gcagtcggcg gccagctcac cctctccgga gcctccgagg tgctcctgct
     2461 gctcagcgcc gccaccgatt acagcggctt cgccggccgc catacccccg atccggaggc
     2521 ggccgcccgg caggatctcg agcgggcttg cgctcgggat tatgccggcc tgaaggcgcg
     2581 ccacatcgcc gactatcaat cctttttccg ccgcgtcgcc cttcgtctcg agccgcgcag
     2641 ccccgcggaa aagatggctg ctcagcagcc caccgaccgc cgactcacca acctccagca
     2701 gggggggagc gatcccgccc tcatggccct ctatttccag tacggccgct atctgctcat
     2761 ctcctcctcg cggccgggcg ggctccccgc caacctgcag gggctgtggg ccgaggagat
     2821 ccagaccccc tggaacggtg actatcacat caacatcaat ctgcagatga attactggcc
     2881 ggccgaggtg ggcaacctcg ccgaactgca tacgcccatg ctcgattata tctgcgcttt
     2941 gcaggagccg ggccggcgca ccgcccgcgc ttactataac gcccgcggct gggtggcgca
     3001 cgtgatcagc aatccctggc tctttacctc gccgggcgag ggagccgcgt ggggcgccac
     3061 ggtcagcggc gcggcctggc tctgccatca cctctgggag cactatgcct tttcgggcga
     3121 ccgccgcgtg ctggaaaaaa tctatccggt cctgaaagag tccgccctct tttacagcga
     3181 cctgctcatc cgtgagccgc agcacggctg gctggtgact gccccctcca attccccgga
     3241 gaacagctac cgcacccgcg atggcttttc cgggcagatc tgcatggggc ccgcaatcga
     3301 ccagcagctc ctgcgcgcgc ttttcggcag ctgcatcgcc gcggcggaga gcctcggcgt
     3361 cgacgccgga ttcagcgccg ccctcgattc cattcgccgg gagctggcgc ccaatcagat
     3421 cggctcgcag ggccagctgc tcgagtggct ggaggagtac gaggagcccg aaccgcacca
     3481 tcgtcacgtc tcccatctct gggggcttca tcccggtgac gagatctcgt tcgaatccac
     3541 tcccgagctg gccgcagcgg cgcgcaaaag cctcgaacgg cgcggggatg acggcaccgg
     3601 ctggtccctg gcctggaaga tcaatttcca ggcccgcctc ggcgagggcg accgcgccct
     3661 gaccctgctg cgccggctcc tgcagcccaa cggcgcccga tgggcgcagg gcgagggggg
     3721 cggcagcggc acttatccca accttttctg cgcccatccc ccgttccaga tcgatggcaa
     3781 tttcggcggc gcggcgggca tcgccgagat gcttctgcag agccagggcg gcatgctgcg
     3841 cttcctgccc gccctgccgg gggagtggcc ggagggagag gtcaccggcc tgcgcgcccg
     3901 cggcggcttc accgtcgaca tccagtggcg gcaaggccgg ctcaagcgcg cccgcattca
     3961 ttcggccaat ggagctccat gcgtcatccg ctggcagggt gagcccttca agatccgctc
     4021 gagcgacggc cggcctgtcc gcgtcagctt tgaggagggg tttatccgct ttgcatgcac
     4081 gaagaattcc accacgatca tcaccttcaa accgtcccag aggaagtcat gagatccatc
     4141 atccttttaa tgctgcttgc cctcaccctt cctgctgccg ccctggagat gaccgcgccg
     4201 gcccaggagg tggaagcctc tgtcctccgg gccgcccgtg tgaccccctc gccgcgccag
     4261 tacgcctggc aacagctcga gaccattgcc tttgcccact ttggcatcaa taccttcacc
     4321 gatcgcgagt ggggtgaagg gagcgaggat cctggccttt tcaaccccga ggccttcgat
     4381 gcgcgccagt gggcgcgggt gtgcaaggag gcgggactga agatgatcat catcaccgcc
     4441 aagcatcacg acggcttttg cctctggccg agcgctttta cggaccattc ggttaaaaaa
     4501 tcgccctggc gcggcggcag gggggatgtc gtgaaagagg tcgccgaggc ctgccgggaa
     4561 tacggcctca aattcggttt ttacctctct ccctgggacc gccatgaggc cagctatggc
     4621 gattctctgc gctataacgc ctttttcatg aaccagctgc gcgagctgct caccggctat
     4681 ggcgaggtca gcgaggtctg gttcgatggg gcctgcggcg aggggcccaa cggcaagcgc
     4741 caggtctacg attttcaggc ctggtaccgc ctcattcgcg agctgcagcc caatgcggtt
     4801 atcgccatca tgggtcctga tgtgcgctgg gtcggcaccg aagccggcta tggccgcacc
     4861 acggagtgga gcgtgctccc tgctgcggct caaaataccg acagcatctc cgcttcctcc
     4921 cagcaggagg cctccgaccg ggcctttgtg cccggcgacc tcacgggtga ggatctcggc
     4981 agccgcgaaa agatccggga agccggccgg ctggtctggt atcccgccga ggccgatgtc
     5041 tcgatccgcc cgggctggtt ctatcatgct gaacaggacg ggcaggtcaa gagcccggaa
     5101 aagctggtcg atatctttta cggctcggcc gggcgcaact gcgtgctgct gctaaatatt
     5161 ccgcccgacc gccgcggcct gatccacgaa aacgacgccg ccgcgctgcg cgaaatgcgc
     5221 cggcgcctcg atgccacttt tcagagcaat ctcctcgccg gggcgcgctt gtcggccgac
     5281 agcggcagcg atccatgcgc ggccgcagat ggcagcctga atacaggctg gcgcgccggc
     5341 gatggccgcc acagcgccat cctcactgcc gacctgggcg ccaggaaaac cttcgatgtg
     5401 ctcgccctgc aggaggatat cctcaccggg cagcggatcg aggcctttga gctgcaggtg
     5461 gaggagggcg cccgctggcg cagcgttgcc tatggcacca cagtgggcta caagcggctc
     5521 ctccgcttcc cgcccgtcac agccgccagg gtgcagctgg tgatcagccg ttcccgcacc
     5581 gagccggcac tggcggaatg ggggctctac aagcagccgc cggaagagga actcagggca
     5641 gcgggaggcc ctgtgggcaa aaagagcgac atccccggcc tggctttgaa acaggaggca
     5701 tcgccgcccc tgttattggc ccgcagggga ggctcctcct accgcattgt gcaccctgaa
     5761 aaggcgtcgg ctgcggagca gcgcgctgcg ggcctcctgc aggagtacat gcgcaagtcc
     5821 accggggccg agctgcccgt cgtcagcgag acctcaccgg caacagagcg ggagatcctc
     5881 atcggcgccg tccgccgtct gcctgcacgc ctggcgcagg tgcagaatca gctctccggg
     5941 gatgcctttt tcatcggcca cgaagggcgc aagatcattg tgctgggggg agcggatcgg
     6001 ggtgtggtct acggcgccgt ccattttctc gaaaaatacc tcggctgccg caggtacacc
     6061 cccgaggttg aggtggtgcc ccggcatgaa gagctgagcc tcgccgtcgc cgaggagatt
     6121 caggcgcccg ccaaccgctt ccgctccatc aacacccgct ttgcccgcga tcccggctac
     6181 aaggagtggc tccgcctcga tgaggtccag gatatctacg cccgcggctg gtacgtccat
     6241 accttcgacc agctggtgcc gcgtgcggag tactttgccg atcatcctga atactattgc
     6301 tggatgaacg gcaagcgcat ccacgatcag ctctgtctca gccatcccga ggtgctgcgc
     6361 atcacccttg accggctgag caaggcgatg gcggagcagc ccgacaaaaa gtactggtcg
     6421 gtgagccaga acgacaattt ttcctactgc cagtgccccg cctgcagtca ggtcatcgcc
     6481 gaggagggct cgcccgccgg gccggtcatc cgtttcgtca atgccgtggc ggcggctttt
     6541 ccggacaagg tcatctccac cctcgcctat cagttttcac gtcaggctcc caaaatcacc
     6601 cgtccggctg aaaatgtcca tatcgtcctt tgcaccatcg aactcaaccg cagccagccc
     6661 atcgagagtg atccgggcag ccgggatttc gtccgggata tcgaggagtg gtcgaggttg
     6721 acgaaaaacc tctacatctg ggattatgtt gtccagttca ccaattttgt cagccccttt
     6781 cccaatctgc acgtgctcca gcccaacctg cagttctttg tccgcaacgg cggctacgag
     6841 cattttcagc agtccaatgg ccagtccggg ggcgagatgg cggaactcaa gaactggctc
     6901 atcgcgaaat ggctttggga ccccgatctt gacgccgatt ccctgaagga ggatttcctg
     6961 cgcggatatt acggtccggc ggcgcccttc attcaggagt acgccgcccg tctggaggcg
     7021 gagctgctcc gctccggtga ccggctggac atctatggct ctccggtctg gcatgcgggg
     7081 agcgtgctct cggcggagaa tgtggcggat tacaaccgga tgttcgacca ggccgaagcg
     7141 gccgtggccg gggaggaggc gctgctgcag cgggtgcgga ccgcgcgcat gcccctccag
     7201 ttcgcgatca tggagatcgg caagaacgac ctcttcggcc cgcggggctg gtaccgggag
     7261 gagggaggaa gctttctgct gcgcccggag atgcgcggcc tcctggagga gttttatgcc
     7321 gccggcaaaa gcgccgggct gcgcctggtc aatgagagcg gcttcaccct cgatgactat
     7381 taccggacca ccctgcgctt catcgagtcc agggtggagg gcaacctcgc gttccgcaaa
     7441 acagtgagtg ccgatccccc gccgagcccg aaatacgccg gcggcgatcc cggcgtgctc
     7501 accaacggcg tgcagggggc gagcgactac aaggtgcact ggctcggttg ggagggggtg
     7561 gattttcagg tcaccctcga cctcggcagc cccgcctcac cggcacgcgt ccgtctggcc
     7621 accctctacg atcccaagag ctggatcctc catccccgcc gggtgacctg cctggcctcg
     7681 gccgacggct tccgcttcga gcccgccggc agcctgacag tcgatggaga ccagcggcag
     7741 gaggagaagg tgcgcgagtt tctcttcgac gccctgccgg ccggggtgcg ctggctgcgc
     7801 tttgaggtgg agggcacccg gcatctaccc gcctggcacc cctcggaggg cgggctctcc
     7861 tgggtcttca tcgacgagat cgtggtcgaa tcgctggcag gaaccggcga aaaagaatga
     7921 attgtgtgca gcggctgcgg gagcactgcc gggaaccccg ttcttgaggg cgcctggcag
     7981 ctgttgcagc ccggataagc atgaacaaaa agaaaaggag gaaa
//
LOCUS       DAOPWU010000149         7953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9097_length_7953_cov_4.586731, whole genome shotgun sequence.
ACCESSION   DAOPWU010000149 DAOPWU010000000
VERSION     DAOPWU010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9097_length_7953_cov_4.586731"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKM23_08740"
     CDS             <1..244
                     /locus_tag="PKM23_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY91600.1"
                     /translation="MLLMEALPFVDEAEKFQRVELQSLNRAVAQLEKEMIERALRRFA
                     GNQSRAAQVLHLSEPNLRFKMKKLGIRKEEFIMGNE"
     gene            403..2535
                     /locus_tag="PKM23_08745"
     CDS             403..2535
                     /locus_tag="PKM23_08745"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNY91601.1"
                     /translation="MRLTRVFIMLCLLASLGFAEEGMFPMNELKRLDLRQAGFELSAE
                     AIFNPDSVSLTTAIINIGGCTGSFISPQGLILTNHHCAFSAIQRASSAEYDYLREGFY
                     AASHEKEFPARGFTVRITDSYVDVSQEILAAVAAAGDFSTRAKAKEKRGKELIAAAEK
                     ENPGKRVELSEMFPGKSYMLFIYTYLTDVRLVYAPPFGIGNFGGEEDNWVWPRHTGDF
                     ALMRAYVAPDGKPAEYAPGNVPYSPRVYLPIASRGAREGDLAFILGYPGRTYRHTSSH
                     YLEYESRLRMPWIVEWYGHQIKTMEEMSRKDRTVALKLSSPIKGLANTWKNYQGKLLG
                     IDRLDLVERKRLEEARLAEFITADSARQSRYGTVLPEIDAYYKEMSSRFESDKLMDAL
                     LRSSSLLSTAYTLYQSSTEMAKPDLERDSPYMERNLTRTRERLPLALADFYLPRERLF
                     LGEIIEKIQALPAREQPAAIARLPKGKKLGKFLDEAFAGSKLMESAWAMQLFGKPAGE
                     LIGTGDPFLKLAAGLHPDLERQREFRRASGARLEKLMADYVEIKQAAAGRDFIPDANS
                     TLRLTYGHIRGYAPRDAVWYAPFTTLNGILEKNRGEEPYNAPRALLEAGANSGASPYV
                     NPDLGAVPVNLLYDMDTTGGNSGSPVLNQRGELIGLNFDRAFEATINDYAWSSSYSRS
                     IGVDVRYILWILAEVSGAKGLLNELGVE"
     gene            2564..3733
                     /locus_tag="PKM23_08750"
     CDS             2564..3733
                     /locus_tag="PKM23_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HNY91602.1"
                     /translation="MYKKIAALFTLMALFLLSCQDAAYLDLGKKAVEVDSLIIKARAA
                     GLSNMDSLRAARRDKALSYIAGVNPERVRAENYHAAARLFFAAGKADSARLLLEKYAP
                     AGSDREALDLLFSLYLQKGDPVKAEQLFRDKLANLAGEEAGEYYLNLLYGYQEQDQLD
                     KAIAVADAGIAALPPETKGSLVVEKADMLHQKGDRTAALALLDDLKKSIGAEDRLQRA
                     IAAKTTLFNLIGAPAKEWRAAGWIDSGPMLLKDLRGKVILLDFWAPWCGPCRAMFPHL
                     KKLYGEYHDQGLVIIGLTRCYGRFNQLGQNLRDLAPATELEWITKFKEHHQIPFPYAV
                     ADAEEAAANEAAYGVYGIPHMVVIDKKGVVQEYAIGSGPSSEKKLDDAIARLIAE"
     gene            3758..4507
                     /locus_tag="PKM23_08755"
     CDS             3758..4507
                     /locus_tag="PKM23_08755"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNY91603.1"
                     /translation="MLEQFFLAERPLLLAHRGASGHAPELTRAAFKRAIELGADGIEL
                     DVQLSRDDHLVVIHDDTLQRTTNGSGRVDQHLRRELEKLDAGSWFGPGFSQERIPDLS
                     EILHFGPPGWRLNIELKSVPEPVRLAEKTAGLLEGVEDKSRLICTSFDPRILMSLDPI
                     LPGIGLGLIFAGVWPPDELLFRWPVWSAEQSLLSSERIESARARGIRVCAWTVNRVEE
                     IERMITLGVDAIITNYPERFTLAMANLSRKE"
     gene            4570..4643
                     /locus_tag="PKM23_08760"
     tRNA            4570..4643
                     /locus_tag="PKM23_08760"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4604..4606,aa:His,seq:gtg)
     gene            4670..4741
                     /locus_tag="PKM23_08765"
     tRNA            4670..4741
                     /locus_tag="PKM23_08765"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4703..4705,aa:Glu,seq:ttc)
     gene            5043..7181
                     /locus_tag="PKM23_08770"
     CDS             5043..7181
                     /locus_tag="PKM23_08770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY91604.1"
                     /translation="MFHNNPFYHVINGQCMTLSIIIVSYNVREFLEQALISIERAVAR
                     IDHEIFVVDNNSMDGSAEFVHRRFPGVRLIASPENLGFAGANNLALSEAKGRFICLIN
                     PDTIVQEDTFSTLLTFLEEHPEAGAAGCKILNPDGSLQLACRRSFPTPWVAFTKIIGL
                     AALFPGSSLFGRYNLTFLDEEEISEVEAISGSFMLLRGEAIRQIGPLDDSFFMYGEDL
                     DYCFRIHSAGWKIYYVPATRIIHFKGESSKRSPFEQRRLFYEAMRLFVNKHFRRNSAW
                     IPSWTLIVAIRMRALAALLSAALRSAVWPLLDLLLMTLSLTVAVYLRFSPRFPWEAFL
                     IVHLIYSTIWLVALILQGVYHSWRLSVARTTVAVLIGLVINSAITFFFKEIGFSRLVV
                     LYAGLLNLLLLPGWRMVLKLAAQLHGRLFYRTLGHLALRRTAILVGDRMSSEALASRL
                     RRRLDAAYDVRAIVLPRPEEGITTIEDIPVYTGLAHIHDLLRRERVQEVIFATDRLPY
                     QEMLAIISASTHEQVSFKLVPSSMDVIIGKASVEYIEDMPLMEIHYKLQTPIYRFIKR
                     SLDLVLASLILLLAWPVWLWQRCMAGRRRRPVTYIGERRQSFTVKEWEGSEGGWWRLL
                     PRIAAIWRGRMSFVGPRPESHPASSSLAWSLKPGLTSMEEAGHAPALSEDERERLRLF
                     YMKNYSPFLDAEILIKNAIAGIQKEERSEI"
     gene            7178..>7953
                     /locus_tag="PKM23_08775"
     CDS             7178..>7953
                     /locus_tag="PKM23_08775"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNY91605.1"
                     /translation="MKNYVPEFEKPIVEIERRIAELREYSASKDLSFSREIERLEEKA
                     RQLRQEVYSNLTRWQRVQLARHPNRPYTLDYVRLMMSDFTEFHGDRLFADDPAVVAGI
                     GTLGAISVALIGHQKARDTREKIRRNFGMPNPEGYRKALRLMKLAARFSRPVLCLVDT
                     PGAYPGLGAEERGQAEAIARNLLEMARLPVPIVVVIIGDGASGGALGIGVGDRILMLE
                     NSWYSVISPEGCAAILYRDSANAHIAAESMKVAPEDLLEMG"
BASE COUNT         1608 a         2507 c         2187 g         1651 t
ORIGIN      
        1 catgctgctg atggaagccc tgccctttgt cgatgaggcg gaaaagttcc agcgcgtcga
       61 gcttcagagt ctcaatcgcg ccgttgcgca gctggaaaag gagatgatcg aacgtgctct
      121 gcgacgcttc gccggcaacc agtcgcgcgc cgcccaggtg ctccatctct ccgagcccaa
      181 cctgcgtttc aagatgaaaa agctcgggat ccgcaaggaa gagttcatca tgggcaacga
      241 atagacgctg cttttgccca atgacccgtt tccggccccc gccgtcaagg cagcgggggc
      301 cgtcgttttt tctcccgcca ctactttccg ctttcatctt ttgccaattt tctctatctt
      361 tttgctgcca gtttccgtcc atttatccag caggagtccg tcatgcgtct gacccgagtt
      421 ttcatcatgc tttgcctcct tgcctcactc ggcttcgccg aagagggcat gttccccatg
      481 aacgaactca agcggctcga tctccgccag gccggctttg aactctctgc ggaggccata
      541 ttcaatcccg acagcgtcag ccttaccacc gccatcatca atatcggcgg ctgcaccggc
      601 tcctttatct cgccgcaggg gcttatcctc accaatcatc actgcgcctt ttctgccatc
      661 cagcgcgcca gcagcgctga gtacgattac cttcgcgaag gcttttacgc agccagccac
      721 gaaaaggagt ttccggcgcg cggcttcacc gtacgcatca ccgacagtta tgtggatgtt
      781 tcgcaggaga tcctcgcggc tgtagcggcg gccggcgatt tctccaccag ggccaaggcc
      841 aaggaaaaga ggggcaagga actgatcgcc gccgctgaga aggagaaccc cggcaaacgg
      901 gtcgagctct ccgagatgtt ccccggcaaa agctatatgc tcttcatcta cacctatctg
      961 accgatgtcc gcctcgtcta cgccccgccc ttcggcatcg gcaactttgg cggcgaggag
     1021 gacaactggg tctggccgcg tcataccggc gatttcgcgc tcatgcgcgc ctatgtcgct
     1081 ccggacggca agcccgccga gtatgccccg ggaaatgtcc cctactcacc gcgcgtctat
     1141 ctgcccatcg cctcccgcgg agcccgcgaa ggcgacctcg ctttcatcct cggctatccc
     1201 ggccgcacct atcgccatac cagttcccac tatctcgaat acgaaagccg gctgcgcatg
     1261 ccctggattg tcgaatggta cggacaccag atcaaaacca tggaggagat gagccgaaag
     1321 gaccgcaccg tcgccctcaa gctctcctcg ccgatcaagg gactggccaa cacctggaaa
     1381 aattaccagg gcaagcttct cggtattgat cgccttgacc tcgtcgagcg caagcggctg
     1441 gaggaggccc gcctggccga attcatcacc gccgactcgg cacgccagag ccgctatggc
     1501 actgtactgc cggaaatcga cgcctactac aaggagatgt cgagccgatt cgagtccgac
     1561 aagctgatgg atgccctgct cagaagttca tctctgctct ccactgccta caccctctac
     1621 caatcctcca cagagatggc caagccggat ctcgaacgcg acagccccta catggagcgc
     1681 aacctgaccc gcacccgcga acggctgccg ctcgctctgg ccgatttcta tcttccccgg
     1741 gaacgcctct tcctcggaga aattatcgaa aaaatacagg ccctgcccgc cagggagcag
     1801 ccggcagcga tcgcgcgtct tcccaaaggc aagaaactgg gcaaattcct cgatgaagcc
     1861 tttgccggca gcaaactcat ggagagcgcc tgggcgatgc agcttttcgg caagcccgcc
     1921 ggagaactga tcgggaccgg ggatcccttc ctcaagctgg cagccgggct tcatcccgac
     1981 cttgagcggc agcgtgaatt ccgccgcgcc tccggtgcca ggctcgaaaa actgatggcc
     2041 gactatgtcg agatcaaaca agcggccgcc ggccgcgatt tcatacccga tgccaacagc
     2101 accctccgcc tgacctatgg gcacatccgc ggctatgccc cgcgcgatgc cgtctggtat
     2161 gctcccttca ccaccctcaa cggcatcctc gaaaagaaca ggggggagga accttataat
     2221 gcgccccggg cactgctcga agcgggcgcc aacagcggcg ccagccccta tgtaaacccg
     2281 gatctggggg cggtaccggt gaacctgctc tatgacatgg ataccacggg cggcaattcc
     2341 ggaagtccgg ttctgaatca gcgcggggaa ctgatcggcc ttaattttga ccgggcattc
     2401 gaagcgacga tcaatgacta tgcctggagc agctcctaca gccgttcgat tggcgtcgat
     2461 gtccgctaca ttctctggat cctcgccgag gtctcggggg cgaagggact gttaaatgaa
     2521 ctgggtgttg aataaataac ctgcataacc aagggagatg acaatgtata aaaaaattgc
     2581 tgcgctgttc accctcatgg cccttttcct gctctcctgc caggatgcgg cctatctcga
     2641 tctgggtaaa aaggcggtgg aggtggacag cctgatcatc aaggcgcgcg ccgccggtct
     2701 gagcaatatg gacagcctgc gcgccgcacg cagggacaag gctctctcct atatcgccgg
     2761 tgtgaatccg gaaagggtcc gcgctgaaaa ttatcatgcc gctgcccgcc tcttcttcgc
     2821 ggccggcaaa gccgattccg cccgccttct gcttgaaaag tacgccccgg ccggcagcga
     2881 ccgtgaagcc ctggatctgc tcttcagcct ctatctgcaa aagggcgatc ccgtcaaggc
     2941 tgaacagctc ttcagagaca aactggcaaa cctggcggga gaagaggccg gcgagtacta
     3001 tctcaatctt ctttatggct atcaggagca ggatcaactc gacaaggcta ttgctgtcgc
     3061 cgatgccgga attgcagccc tgcccccgga gacgaagggc agtctggttg tcgaaaaagc
     3121 cgatatgctc caccaaaagg gagaccggac ggccgctctc gctcttctcg atgatctgaa
     3181 aaagtcgatt ggcgctgagg atcggctcca gcgcgccatc gcggccaaaa ccactctctt
     3241 caatctcatc ggagctcctg ccaaagagtg gcgggcggcc ggctggatcg attccggacc
     3301 catgctgctt aaagatttac gcggcaaggt gatcctgctc gacttttggg ccccctggtg
     3361 cggcccctgc cgggcgatgt tcccccacct taaaaagctc tacggggagt accatgatca
     3421 gggactggtc atcatcggac tgacccgttg ttacgggcgc ttcaatcaac tcgggcaaaa
     3481 tctgcgcgac cttgcacccg ctactgaact ggagtggatc accaaattca aggagcacca
     3541 ccagatcccc ttcccctatg cggtcgcaga cgcggaggag gccgctgcca atgaagccgc
     3601 ctacggggtc tacggtatac cccatatggt ggtgattgat aagaagggtg tagtgcagga
     3661 atatgccatc ggctccggtc catcctcgga gaaaaaactg gacgacgcca tcgcccggct
     3721 gatcgctgaa taaaacgccg cccggcaagg tgacccaatg ctggaacaat tctttctcgc
     3781 agagcgcccg ctccttctcg ctcaccgcgg ggcatcggga catgcccccg aacttacccg
     3841 cgcggctttc aaacgggcga tcgagctagg tgcggacggc attgagctcg atgtacagct
     3901 ctcccgggat gaccatctcg tcgtcatcca tgacgacacc ctgcagcgca ccaccaacgg
     3961 cagcggccgc gtcgaccagc acctgcgccg tgaactggaa aagcttgacg ccggcagctg
     4021 gttcggaccc ggattttcac aggaacgcat ccccgatctg tcggagattc tgcactttgg
     4081 ccccccgggc tggcgcctga atatcgagct gaagagcgtt cccgaaccgg tgaggctggc
     4141 cgaaaagacg gccggcctcc tcgagggcgt ggaggacaag agtcgactca tctgcacctc
     4201 ctttgatccc cgtatcctga tgagccttga cccgatctta cccgggatcg ggctcggatt
     4261 gatattcgcc ggtgtctggc ccccagatga actgctcttc cgctggccgg tctggagcgc
     4321 cgaacagagc ctgctctcca gcgagcggat cgagagcgcc cgcgcacggg ggatccgggt
     4381 ctgcgcctgg acggtcaaca gggtggagga gatcgaaagg atgatcacgc tcggcgttga
     4441 tgccatcatc accaactacc cggagcgttt cactctcgcc atggcgaatt tgtccaggaa
     4501 agagtaaaat ttacttgatt aaaatctaaa aacatgttaa attaatgctc ttgagtaaaa
     4561 agcgtcgtgg tgggtgtagc tcagttggat agagcaccag gttgtggccc tggtggccgc
     4621 gggttcaagt cccgtcactc accctcccat acatttgacc tgttgcattg tccccatcgt
     4681 ctaggggtca ggactcggga ttttcgatcc cgtaacaggg gttcgaatcc ccttggggac
     4741 actgatccgg gcggcaaatt tttgccgcct tttttttatg gcccggtaaa aaatgaattg
     4801 cgctcattat ggcgtactat tttggctgcc ccccaaaaat ctcggtccca cccaaataaa
     4861 aaaagtatat tacctgccag aaacagcctg ttatataaat cgctttaaaa ccagaccaga
     4921 ggccgggagt atgctggaaa tcgtacagag ggatgagggt acgatgcgcc agatgtaagg
     4981 tggaaaccag atttcggggg ccctggcgta ctattcagtt gcgattcagc agcttttttt
     5041 gtatgtttca taataatccc ttttaccatg tgatcaacgg ccagtgcatg accctgagca
     5101 tcatcatcgt cagttataac gtgcgcgaat ttctcgagca ggcgctcatt tccattgagc
     5161 gcgccgtcgc ccgaatagac catgagattt ttgtcgttga taacaactcc atggacggca
     5221 gcgcagagtt tgtccaccgc cgttttcccg gggttcggct catcgccagc cctgaaaatc
     5281 ttggcttcgc cggcgccaat aacctggcac tctctgaggc gaaggggcgt tttatctgcc
     5341 tgattaatcc cgataccatc gtccaggaag atacattttc caccctcctc acttttctcg
     5401 aggagcatcc cgaggccggt gcggccggct gcaagattct caatcctgat ggcagcctgc
     5461 agctggcctg ccgcaggagc tttccaaccc cctgggtcgc ctttaccaag atcatcggcc
     5521 tggccgccct cttccccggg agttccctct tcggccgcta caacctcacc tttctggatg
     5581 aggaggagat tagcgaggtg gaggcgatct ccggttcctt catgctcctg cgcggcgagg
     5641 cgatccgcca aattggcccc cttgacgact cttttttcat gtacggggag gatctcgact
     5701 actgcttccg aatccactcg gcgggctgga agatctatta tgttcccgcc acccggatca
     5761 tccatttcaa aggggagagc tcgaagcgca gcccctttga gcagcgccgc cttttttacg
     5821 aggccatgcg cctctttgtt aacaaacatt tccggagaaa cagcgcctgg attccctcct
     5881 ggactctgat tgtggccatc cgcatgcgcg ccctcgcggc attgctctcg gcagccctgc
     5941 gcagcgcggt ctggcctctg ctggatctcc tcctcatgac cctttccctg acggtggcgg
     6001 tctatctgcg cttttcaccc cgctttccct gggaagcctt tctgattgtg catctcatct
     6061 acagcacgat ctggcttgta gccctgattt tgcagggcgt ttaccattcc tggcgccttt
     6121 cggtggccag aaccacagtg gccgtgctca tcgggctggt catcaacagc gctatcactt
     6181 ttttcttcaa ggagatcggt ttttcccggc tcgtcgtcct ctacgccgga ctgctcaatc
     6241 tgctcctgct ccccggttgg cgcatggtgc tcaagctcgc cgcccaactc catggacgcc
     6301 tcttttaccg cacacttggt catttggcgc tgcgccgcac cgcgatcctg gtgggagacc
     6361 gcatgagcag tgaggccctg gcttcgcgcc tgcggcggcg gctcgatgcc gcctatgatg
     6421 ttcgtgccat tgtcctgccc cggcccgaag aggggatcac caccatcgaa gacatccccg
     6481 tctataccgg actcgcgcac atccatgacc tcctgcgccg ggagcgggtg caggaagtca
     6541 tcttcgccac cgaccgcctg ccctatcagg agatgctcgc catcatttcg gcctcgaccc
     6601 atgagcaggt gagcttcaag ctcgtgccaa gcagcatgga tgtcatcatc ggcaaggcct
     6661 cggttgaata tatcgaggat atgcccctga tggagattca ctacaaactg caaactccca
     6721 tctaccgctt catcaagcgc agcctcgacc tggtgctggc ctcgcttatc ctgctcttgg
     6781 cctggccggt ctggttatgg cagcgctgca tggctggccg ccgccgccgg ccggtaacct
     6841 acatcggaga gaggcgtcag agttttacag tgaaggaatg ggagggcagt gagggagggt
     6901 ggtggcgctt gctgccgcgc attgccgcca tctggcgcgg acgaatgagc tttgtcggcc
     6961 cccgccccga gagccatcca gcctcctcga gcctggcctg gtccctgaag ccgggactga
     7021 ccagcatgga agaggccggt catgccccgg ccctctctga ggatgagcgc gaacggctgc
     7081 gcctcttcta tatgaaaaac tattcgccct ttctcgatgc agagatcctg atcaaaaacg
     7141 cgatcgccgg catccagaaa gaggagcgga gtgagatatg aaaaactatg tgccagaatt
     7201 cgaaaagccg attgtcgaga tcgaacgccg catcgcagag ctgcgcgagt attccgccag
     7261 caaggatctc tccttcagcc gcgagatcga aaggctggag gagaaggctc gccagcttcg
     7321 tcaggaggtc tacagcaacc tgacgcgttg gcagcgcgtg cagttggccc gacaccccaa
     7381 ccggccctat accctcgact atgtccggct gatgatgagc gatttcaccg agtttcacgg
     7441 cgaccgactc ttcgccgatg atcccgccgt ggttgcgggc atcggcaccc tgggcgccat
     7501 atcggtcgcc ctcatcggcc accagaaagc gcgggatacc cgggagaaaa tccgccgaaa
     7561 tttcggcatg cccaaccccg aaggctaccg caaggcgctg cgcctgatga agctggctgc
     7621 ccgcttctcc aggccggtgc tctgccttgt cgacaccccc ggcgcctatc ccgggctcgg
     7681 cgccgaggag cgcggccagg ctgaggcgat cgcccgcaac ctcctcgaga tggcccgtct
     7741 gccggttccg atcgtggtgg tcatcatcgg cgacggcgcc agcggcggag ccctcggcat
     7801 cggggtggga gatcggatcc tgatgctcga gaacagctgg tattcggtca tctctcccga
     7861 aggatgtgcc gccatcctct accgggacag cgccaatgcc cacatcgccg cggaatcgat
     7921 gaaagtggcc cccgaagatc tgctcgagat ggg
//
LOCUS       DAOPWU010000150         7891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9190_length_7891_cov_4.806534, whole genome shotgun sequence.
ACCESSION   DAOPWU010000150 DAOPWU010000000
VERSION     DAOPWU010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9190_length_7891_cov_4.806534"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3237
                     /locus_tag="PKM23_08780"
     CDS             <1..3237
                     /locus_tag="PKM23_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY91606.1"
                     /translation="LALDSMSVILTLSPLAENTPCTLTVTGVQDLATLPNPIVEPEAI
                     PFTFKPFLPNTIAAWDMNDIDLTTLTDYSGSANHATLFNGLGYAAGQSGNGLIFDGID
                     DYARIPASPTLDIKGEAVSLSLWARLDYLPAELPGSYGPTYDSQGDNYVIYEDKGNNE
                     LRFKVTTNKGAERPGIPAADLRKNVWLHIVGVYDGAKAMIYMNGQLKDSHNLIGTVNP
                     GQIGLIGDSPGSYFKGSIDEIQIFNRALAPEEVLFLYKEAKTAIIDAEPPLLTRAASV
                     GDSQRLYVSFNEPVDQTSAENIAHYAIDNGVSVEAAQLAVDGRSAILTTSPFSPGMVY
                     TLSVTGIEDLAEEPNLIAPNSSAQFVHKEFPDGLVSSWLMDEGADTTSVDGTANANTM
                     FLRNGAEWSPGEYGNGLSFDGIDDYAIVHNSPSLDIDTSGVTVSAWVKLNFLPADQPT
                     TFAPIFDCITDNYVLYVDKNNKELRFKVTTTKGAERPGIPAADLKVGEWLHVVGVYDG
                     GSARIYLNGELKDVHAGLTGNIKPGTIITLGVEGGSRFSGSLDHVQLFNRGLSREEVI
                     WLYAGLQVPHLTVESVTQTAVTLAWNPVSDPLLGLSGYKVYRDTTKAPATLLASLGDT
                     THYVDETKSEAITYHYRIRSVDGSGVESPFFSNEVEVMTGSDTTPPQLLQVMSTGEPG
                     RVLAQFSEKVDPASATAAAHYTLDNGGGVDSVRLALDGRTVLLSVSGLTGGTVYTLTV
                     DQVADLATVPNLIPAGSNRIFVYHAPLAGLIAYWPLDEEADSTAFDVSGLGNHGKLGG
                     APLRSPGLFGNALSFNGYDQFVAIPNSPSLNIDTSAVTLSLWVKLVDLPTAMPIGIGS
                     IYDAAQDCYVIYADKGNKELRFKVTTDKSAERPGIPEAKLTTGQWLHIVGVYNGSQAM
                     IYLNGELMDTHTLTGRVKPNQVARLGNDGANFFRGEIDNVQIYRAGLSADDVRYLYTG
                     IKRTTAVEEKPALPEVCSLSQNYPNPFNPVTTLQYEIAQKGLVRMTVYDLLGRRVADL
                     VNRVQEAGRYQVSWNGVDESGMPAGSGVYLCRLTSGSYTALRKMILVR"
     gene            complement(3387..3917)
                     /locus_tag="PKM23_08785"
     CDS             complement(3387..3917)
                     /locus_tag="PKM23_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91607.1"
                     /translation="MKKRSAFYPGVFALAMGMLLVSSLAQEKGAQGPPPARKIPGITA
                     PDAFPNACVDCHLNYAEMQMDTRFSTLLQRLCEKVEPGLLAKAQAAAPKALMLEGQHP
                     EVGDIFDNVPASCLSCHGEGSETSPPFSKMIHAIHLTGGEANHFLTLFQGECTHCHKL
                     DQATGLWTIPSGAEKK"
     gene            4679..7072
                     /gene="sppA"
                     /locus_tag="PKM23_08790"
     CDS             4679..7072
                     /gene="sppA"
                     /locus_tag="PKM23_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNY91608.1"
                     /translation="MKKWILMFWLAAGIPGMLQAQPSFPRYPRSAAFLHASPGAFDEG
                     MLGYVNPAATALLLTPEARLFFGADAGHLSANREWGLLAGAPNLGLGWVRRGDVDEYH
                     TSLAGGGEALAIGLGYGWTKGGLQEQRMVSAGALIRPSRYASIGLSGLFSLDDAQREG
                     VLDLALRPLGSDRLTLFGDLAMADHDNLKTAPYSLGAVVKLLPGLHLTARWFDSQAWS
                     TGLSFSLGSAGLYARRAIAGDDQADRDYYGLRLGGKRESILLRRNTRYYAQNIRGGIR
                     YQKFRWFDDESLSLSTLLADLQGVIEDPAVAGVALNLSGMAANHELVWELREKLKEVR
                     RADKKIIVFLERGGMTEYHLASIADRIVLDPEGMLQLQGYIMGRTFQKELLEKVGLGF
                     DEWRFFKYKSANESLSRDSMSEADREQRQALLDDLYAVVREDICTSRHITPARFDSLI
                     NTAVLFDAADALRYGLADTLGRWIDADKIVESIEKGKKPFISAGQLAKYTMHRPEWGG
                     RPKIALVYAIGVCDLESGIQARKLEKTFKKLTEDKSIRAVVLRVDSPGGDGLASDLVA
                     EAMKRCAGEKPVIVSQGSLAASGGYWLSMYADTVVAAPYSLTGSIGVIGGWVWNKGLG
                     EKLGLRADHVQVGDHADLGFGIQLPLLGVQVPDRNLTPEERDRMEMMIRAMYEGFVGK
                     VAAGRGLSPDSVGQIAQGRVWSGLAAQKIGLVDVIGGMEKAIALAREAAGIPAGREIT
                     IVEYPRPALFDFSVWMPRLLGSVLPVAREDAEIDYLKKIARHPGQPLPLLTPDYYLQ"
     gene            complement(7139..>7891)
                     /locus_tag="PKM23_08795"
     CDS             complement(7139..>7891)
                     /locus_tag="PKM23_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91609.1"
                     /translation="KMILAGYSNGVPTTIALVNEETQLPTYLRNIGGYIPIDNAMKVD
                     GGAMKEALIWYSTTYFDLVAAGNGGEPVYFAAMADAVRNNPDGHDFSPDMTNLGFVLN
                     MVAGPTLFPGHPFHYWAATWADGKPTGLRFTDWELWLDFLASGTAWEPYIWTAEWCGY
                     VAGTFDTPYDDHLGEIRVPILNLTPAGGYADATFYGLSLLGSRDKETIMPSMGGPVEQ
                     EFAHIDLFTAPEAVNLVWQPMLRWIRMHTPGY"
BASE COUNT         1554 a         2569 c         2289 g         1479 t
ORIGIN      
        1 ttggccctgg actcgatgtc ggtcatcctc accctctccc ccctggcgga gaacaccccc
       61 tgcacactga ccgtgaccgg ggtgcaggac ctcgctacac tgcccaatcc catcgtcgaa
      121 cccgaggcga tccccttcac cttcaaaccc ttcctgccca acaccatcgc cgcctgggat
      181 atgaacgaca tcgacctgac caccctgacc gactattccg gcagcgccaa ccacgccacc
      241 ctcttcaacg gcctgggcta tgcagccgga cagtcgggca acggcctgat cttcgacggc
      301 atcgatgatt acgcacgaat acctgcgtcc cccacgctcg acatcaaggg cgaagcggtg
      361 agcctctcgc tctgggccag gctcgattac ctgcctgccg aactgcccgg ctcctacggg
      421 ccgacctacg actcccaggg cgacaattac gtcatctatg aagacaaggg caacaatgaa
      481 ctgcgcttca aggtgacgac caacaagggc gcggaacggc cgggcatccc ggcggccgat
      541 ctcaggaaaa atgtctggct tcatatcgtc ggggtctatg acggcgccaa ggcgatgatc
      601 tacatgaacg gccaactcaa ggattcccat aatctgatcg gcacggtcaa tcccggccag
      661 atcggcctca tcggcgacag ccccggctcc tatttcaagg gctccatcga cgagatccag
      721 atcttcaacc gcgctctggc ccccgaggag gtcctcttcc tctacaagga ggcgaaaacc
      781 gccatcatcg atgctgaacc cccactgctc accagggcgg cctcggtggg cgattcgcag
      841 cgcctctacg tctccttcaa cgaacccgtg gaccaaacct ccgcggaaaa catcgcccac
      901 tatgcgatcg acaacggcgt cagcgtggag gctgcccagc tggcggtcga cggccgcagc
      961 gctatcctga ccacctcgcc tttctcaccg gggatggtct ataccctgtc ggtcaccggg
     1021 atcgaggatc tggccgagga gcccaatctc atcgcgccca acagcagcgc ccaatttgtc
     1081 cataaagagt ttcccgacgg tctggtcagc agctggctga tggatgaggg cgccgatacc
     1141 acctccgtgg atggaacggc caacgccaac accatgtttc tgcgcaacgg cgccgagtgg
     1201 tcaccggggg aatatggcaa tggcctgagc ttcgacggca tcgacgacta tgccatcgtg
     1261 cacaattcac ccagcctcga tatcgacacc agcggtgtga ccgtctcggc ctgggtcaag
     1321 cttaatttcc tgccggccga ccaacccacc accttcgccc ccatctttga ctgtatcacc
     1381 gacaactacg tcctctatgt cgacaaaaac aacaaggaac tgcgtttcaa ggtgaccacc
     1441 accaagggcg cggagcggcc cggcattcct gccgcggacc tgaaggtggg cgagtggctc
     1501 cacgtcgtcg gcgtctatga cggcggcagc gcccgcatct atctcaacgg cgaactcaag
     1561 gatgtccacg ccggcctcac cggcaatatc aagccgggaa cgatcatcac cctcggcgtg
     1621 gagggcggat cgcgcttcag cggctccctc gaccacgtcc agctcttcaa ccgcggcctc
     1681 agccgcgagg aggtgatctg gctctacgcc ggcctccagg tcccccatct gacggtggag
     1741 tcggtcaccc agaccgcagt caccctcgcc tggaatcccg tctccgatcc cctgctcggc
     1801 ctctccggct acaaggtcta tcgtgacacc accaaagcgc cggccacctt gctggcgtca
     1861 ctcggcgaca ccacgcacta tgtcgatgag accaaaagcg aagccatcac ctatcactac
     1921 cgcatcagaa gcgtcgacgg ctccggggtc gagagcccct tcttcagcaa tgaagtggaa
     1981 gtcatgaccg gcagcgacac cacgccgccc cagctgctgc aggtgatgtc caccggtgaa
     2041 ccgggacggg tgctggctca gttcagcgaa aaggtcgatc cagcctctgc caccgcagcg
     2101 gcccattaca ccctggacaa cggcggcggc gtcgattcgg tccgcctcgc cctggacggc
     2161 cgcacggtgc tgctctccgt cagcgggctg acaggcggca cggtctatac cctgacggtc
     2221 gaccaggtcg ccgacctggc gacggtgccg aaccttatcc cggcaggcag caatcgcatc
     2281 ttcgtctatc atgcgcctct cgccggcctc atcgcttact ggccgctgga tgaggaggcc
     2341 gattcgacgg cgttcgatgt ctccggactc ggcaaccacg gcaagctcgg cggcgccccg
     2401 ctgcgcagcc ccggcctctt cggcaacgcc ctgagcttca acggctatga ccagtttgtc
     2461 gccataccca actcgcccag cctcaacatc gataccagcg cagtcaccct ctcgctctgg
     2521 gtcaaactgg tcgacctgcc caccgccatg cccatcggca tcgggtcgat ctatgacgcc
     2581 gcgcaggact gctatgtgat ctatgcggac aagggcaaca aggaactgcg cttcaaagtg
     2641 accaccgaca agagcgccga acggcccggc attcctgagg ccaaattgac gacgggacag
     2701 tggctccata tcgtcggggt ctacaacggc agccaggcca tgatctacct caacggcgag
     2761 ctgatggata cccacaccct caccggcagg gtcaaaccga accaggtcgc gcggctgggc
     2821 aacgatggcg ccaatttctt ccgcggcgaa atcgacaacg tccagatcta tcgcgccggc
     2881 ctgagcgccg acgacgtccg ctacctctac accggcatca aacgcaccac cgcggtggag
     2941 gaaaaaccgg cgctgccgga ggtctgcagc ctctcgcaga actatcccaa tcccttcaat
     3001 ccggtcacga ccctgcagta cgaaatcgct caaaagggtc tggtcaggat gaccgtctac
     3061 gatctgcttg gacgccgcgt ggctgacctg gtaaaccggg tgcaggaggc ggggcgttat
     3121 caggtgagct ggaacggagt ggatgagagc gggatgccag cgggcagcgg cgtctatctc
     3181 tgccggctga ccagcggcag ctacacggcc ctgcgcaaga tgatcctggt gcgctgatca
     3241 cttcatgata cctgtacata aaacgaaagg cgccgtttgt cggcgccttt tttttatcct
     3301 tatacccttt gcttttactt cagatgcagc agaccggatc ccggggataa actgtccggt
     3361 caaaatagag gcagagcgcg ggcgggttat tttttctcgg ctccgctcgg aatcgtccac
     3421 agccccgtgg cctgatcgag cttgtggcag tgggtgcact cgccctggaa gagggtgaga
     3481 aagtggttcg cttccccccc ggtcaggtgg atggcgtgga tcatcttgct gaagggcggg
     3541 gaggtttcgg atccctcacc atggcaggag aggcagctgg ccgggacgtt gtcgaatata
     3601 tccccgactt ccgggtgctg gccttccagc atcagagcct tgggggcggc ggcctgtgct
     3661 ttggccagga gtcccggctc aaccttctcg caaagtcttt gcaggagcgt gctgaagcgg
     3721 gtgtccatct gcatctcggc atagttgagg tggcaatcga cgcaggcatt ggggaaggcg
     3781 tcaggggcgg tgatgccggg gatcttgcgc gccggcggcg gcccctgcgc tcccttttcc
     3841 tgcgccaggc tcgaaaccag cagcatgccc attgccaggg caaacactcc agggtagaac
     3901 gcgcttcttt tcttcatgac atctcctttc ctcattgctt gctgccgagt cggctcagcc
     3961 agtataccgc cggcgccggg catcaaccgg cgaacgcgaa cagcagcggt gaaatacaaa
     4021 tcggaggcgg cgtggaggtc tagtctgatc gaaggggggg ctaatcagcg gagttcctga
     4081 acaatcccgg cgcggtaaag cggacccaat cttattatac tccatttttc tgcatcaggc
     4141 aaggggaaaa ttattcttca aggtaccccc gaaatctggc tcccacctga caccttgtgc
     4201 cgcatacccc gaagatctgg cacccacctg ataccttgcg ctgcgtaccc cgaaaatctg
     4261 gcacctacct gataccttgc gccgcgaacc ccaaaaatct ggtacccacc cgacaccttg
     4321 tgccgcatac cacgaaaatc tggcacccac ctgatacctt gcgctgcgta ccccaatcct
     4381 tccttatcct ttccagcata tttgcggcct gtgctctcga caaggaggca atctttttac
     4441 aggctgtttc tggcaggtac aaaacattta ttattcaatt ccggggagga acgagatttg
     4501 gaagactagt caattttggg tgggaacgag atttcggggg gtagcctgga catggtcgag
     4561 taaacaggtg cagattgaca gcaatttccg cttgcttcat gatcattttt aaagtatact
     4621 ctaattgata tcaattagcg cccaccctgc agttttcaag ggaacggaag gagaacagat
     4681 gaaaaaatgg attttgatgt tttggctggc ggcagggatt ccgggcatgc tccaggccca
     4741 accctccttt ccgcgctacc cccggagcgc tgcttttctc catgcctccc ccggggcttt
     4801 cgacgagggc atgctcggct atgtcaatcc ggcggcgacg gctttgctgc tgacccctga
     4861 ggcccgcctc tttttcggcg cggatgccgg ccatctctct gccaaccggg agtggggcct
     4921 gctcgccggc gcccccaatc tcggtctggg ctgggtccgc cggggcgatg tcgatgagta
     4981 ccatacctcc ctcgccggcg gcggcgaagc cttggctatc ggcctcggct acggctggac
     5041 caaaggtggg ctgcaggagc agcgcatggt gtcggccggg gcgctcatcc gcccgagccg
     5101 ctatgcctcc atcggcctca gcggcctctt ctctctcgat gatgcgcagc gcgagggggt
     5161 gctcgatctc gccctgcgcc ccctaggcag cgaccgcctc accctcttcg gcgacctcgc
     5221 tatggctgat catgataatc tcaaaaccgc cccctacagc ctgggtgcgg tggtcaagct
     5281 cctccccggc ctccacctca cggcccgctg gttcgattcc caggcgtgga gcaccgggct
     5341 ctccttcagc ctgggctcgg ccgggctcta cgcccgccgg gctatcgccg gggatgacca
     5401 agccgaccgc gattattacg gcctccgcct cggcgggaaa agggagagca tcctgctgcg
     5461 caggaacacc cgctattatg cccaaaatat ccgcgggggc atccgttacc agaaattccg
     5521 ctggttcgac gatgaatccc tctccctcag caccctcctc gccgacctcc agggcgtcat
     5581 cgaggacccc gccgtcgccg gtgtcgcgct caacctctcc ggcatggcgg ccaatcatga
     5641 gctggtctgg gaactccgcg aaaagcttaa ggaggtgcgc cgggcggata aaaagatcat
     5701 cgtctttctc gaacgcggcg gcatgacgga ataccacctc gccagcatcg ccgaccgcat
     5761 cgtcctcgat cccgagggca tgctgcagct ccagggctac atcatgggcc ggaccttcca
     5821 gaaggagctg ctcgaaaaag tgggcctggg cttcgatgaa tggcgcttct tcaaatacaa
     5881 atcggcgaac gagtcccttt cccgcgacag catgtcggag gcggaccgcg agcagcgcca
     5941 ggccctgctc gacgacctct acgccgtcgt ccgcgaggac atttgcacct cgcgccatat
     6001 cacccctgcg cgcttcgact ccctgatcaa caccgccgtg ctctttgatg ccgccgatgc
     6061 cctcaggtac gggctggccg acaccctcgg ccgctggatc gacgccgata aaatcgtcga
     6121 gtcgattgaa aagggcaaaa agcccttcat ttcggccgga cagctggcga agtataccat
     6181 gcatcggccg gaatggggcg ggcggccgaa aatcgccctg gtctacgcca tcggcgtctg
     6241 cgatctggaa agcggcattc aggcgcgcaa gctggaaaag acctttaaaa agttgactga
     6301 agacaaaagc atcagggcag tggttttgcg cgtcgactcg ccgggcggcg acggcctcgc
     6361 ctccgatctg gtcgccgagg cgatgaaaag atgtgccggg gaaaaaccgg tcattgtcag
     6421 ccagggcagc ctggccgcct cgggcggcta ctggctctcg atgtacgccg acaccgtcgt
     6481 cgcggcgccc tacagcctca cgggctcgat cggcgtcatc ggcggctggg tgtggaacaa
     6541 ggggttggga gaaaaactgg gactcagggc cgatcatgtt caggtgggcg accacgcgga
     6601 tctgggcttc ggcattcagc tgcccctgct gggcgtgcag gtgccggacc gcaatctgac
     6661 cccggaggag cgcgatcgca tggaaatgat gatccgcgcg atgtacgagg gttttgtcgg
     6721 caaggtggcc gccgggcgcg gcctcagccc cgactcggtg gggcagatcg cccaggggcg
     6781 ggtctggagc ggcctggccg cccaaaagat cggcctcgtc gatgtcatcg gcggcatgga
     6841 gaaggcgatc gcactggcca gggaggcggc cggaattcct gccggccggg agatcacaat
     6901 cgttgaatac ccccggccgg cccttttcga tttctcagtc tggatgccgc ggctgctggg
     6961 cagcgtcctt cccgtcgccc gggaggatgc cgaaatcgac tatctgaaaa aaatcgcccg
     7021 gcatccgggg cagccgctgc ccctcctgac gccggactat tatctccaat agccccgggc
     7081 tgcagcaagg agacaggcag caccggtttg gcggtcagga ggagcgtctg cccgtccgtc
     7141 agtagccggg ggtatgcatc ctgatccagc gcagcatggg ctgccagacc aggttcacgg
     7201 cttccggagc ggtgaagagg tcgatgtggg cgaactcttg ctccaccggc ccgcccatcg
     7261 agggcatgat ggtctccttg tccctgctgc ccagcaggct caatccgtaa aaggtcgcat
     7321 cagcatagcc gccggccggc gtgagattga ggatcgggac ccggatctcc ccaaggtgat
     7381 cgtcgtaggg agtatcgaag gttccggcta cgtaaccgca ccattcggcg gtccagatgt
     7441 agggctccca ggctgtgcct gaagcgagga aatcgagcca gagttcccag tcggtgaagc
     7501 gcaggccggt cggtttgcca tccgcccagg tcgcagccca ataatgaaac gggtgacccg
     7561 ggaagagtgt ggggcctgca accatattca aaacaaagcc aaggttggtc atatccgggc
     7621 tgaagtcgtg cccatccgga ttgttcctca ccgcatccgc catcgcggcg aaatagaccg
     7681 gctccccgcc attccccgcc gcaaccaggt caaaataggt agttgaatac cagatcaagg
     7741 cctctttcat cgctccgcca tcgaccttca tggcattatc gatgggaatg tagccgccga
     7801 tgtttctcaa ataggttggc agctgggtct cctcgttgac cagggcgatc gtggtcggga
     7861 cgccgttgct gtagcccgcc agaatcattt t
//
LOCUS       DAOPWU010000151         7849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9247_length_7849_cov_5.287144, whole genome shotgun sequence.
ACCESSION   DAOPWU010000151 DAOPWU010000000
VERSION     DAOPWU010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9247_length_7849_cov_5.287144"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..2972
                     /locus_tag="PKM23_08800"
     CDS             240..2972
                     /locus_tag="PKM23_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91610.1"
                     /translation="MIRSAYWLILLLLCATLAIPALLTAQTTGKIAGTVKDAASGQPL
                     PGAVVVIEGSKLGAAADIEGSFFILNLSPGVYTLQIRMVGYESMSIRQVRVSVNRTSE
                     ITARLKATVIKGQEVIVTADAIEVKKDQTSSVRNVSADKIEKLPVESLSAVINMQAGV
                     VAGHFRGGRSTEVSYLIDGVQVDEGFSGSGRAVSLEAEAIADLEVITGTFNAEYGRAM
                     SGVVNAVTKEGGNQFHGSFSSSLANYLTPHDDIFIGLNSGRPGLNLSQDYKFQLEGPF
                     FRDKLSFFINGRYQDQNGYLNGIRRFNPWDYSDYSAEDPAEWHIEHTGDNKYVSMDHS
                     QNLSLMTKLAWNATSRIKVTGLYTLNDDMGQGYSHGYKYNPDARGFSFHTAHMGALQL
                     NHMLSQKFFYEVKLSHVDNRYKSYRYENPLDSRFLHGKYYGSGHSGFATGGIDGPGRS
                     MSFYKDSNLKLDATWQATKHHAFKAGLHGIYHDIDQDRIDVRNKWSGTAEENLPVTDA
                     NGKITFPYYELEIVPHTPKTMGIYKKQPYEFSGYLQDKLEYNEMVVNIGLRYDYFNPD
                     QIYPTNRRNPSNQLNLPDSMLTSYKRAEPQTQLSPRLGLAYQLSDQAVLHFSYGHFFQ
                     MPPMYALYENNIFRVPVGDYGTTMGNAQLNAQKTISYEIGVWQELTKGMGLELALFYK
                     DIYDLLSTMVITTYNQIEYGLYTNKDYGNARGLEVKYDFTMGRLSANLNYTLQYTKGN
                     ADNPQQTFTRAGSSMDPIRRLIPMSWDQRHTFNATVNYSDPHYGATLTGYYNSGTPYS
                     FSPMTESPLSLINLYQNNDYKPAGYTLDMTSYYDITVWGKQKVRLTLSVYNLLDRLNA
                     NWVYSDTGQPYTTIVREAQLATHRSDFNDYYDRVKNPTAYSSPRQVKLGMGFVF"
     gene            2991..6575
                     /locus_tag="PKM23_08805"
     CDS             2991..6575
                     /locus_tag="PKM23_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91611.1"
                     /translation="MMRLVCTVLFTLILLSTPLFGQGRPYEGPEDPAGDIAAIRIGYL
                     NGNRIFLAFENNTAFAVGGKPISSKWPNNYEGTVMLDRVSLLIGAEVFVRQDSIPVTD
                     LAEVARLRAEIDTLFFIQSNDPGRNDQNYDATIDWALYPVPGYFNETQDFVAMSNFPT
                     SWPPQGWPAPNFATKWPGEWNGRFGRGIRYADLETYFVANDAQDLENIVRRNDPDQKL
                     IKTGPRYYPRPGHRIGDINPDVTVQRGMPWGGLGLRVSLRGFQWNNPEARDIIFLEYE
                     ISNISAYDLPSCGFGFFLDTAIGGEHSPDGDVGYYNTKLDLAYCWDYDGVGAGGLSPG
                     ILGMAYLESPGKPFDSRDNDEDGLIDEKRDNPAGEKVGPYDGIANLASFLEFYNLQES
                     DLKEHFQGDEDGDWVDGVDLNGNGAYAYQDALGDWYLEPGENPGNDVGLDGVGPMDIN
                     YHGPDEGEGNHRPDFVEGVGCEPNFAATDVSESDMVGLTSFHLFDYYQWTGNNWLVKD
                     DKNVWDLMNDPDFRAYIGEPNSIYFQFSTSKFPFYKGRTERISIAMLHTYENLAGLQS
                     TAHSAPTLFRAKETAQIIYEKDYRFAQPPLLPSLKATAGDGKVILTWDDRADRLTREP
                     FLENINDFEGYKLYRATDKLMSDPEVVTDGQGTRMFRKPIYQCDLLDNISGYADFGLV
                     GGTSFYLGDETGLTHYFVDENVLNGRTYYYALVAYDFGAREVGGGISPTENNIVIELD
                     EAEEIIRMGNNIAVVTPHQNAAGYDDPTVAGEAAETFGSGLVEPLIVDRAAIKPGHTY
                     KLFFRTDTVGYARRNSGMRSSHDLIRCNSGLQVYDVSDGNRLVYKESPARFPAANIQP
                     EGKVLTLDGTVEETFPCYEHTRELFTDVFDGLQLKLSNLTVTGEYDAAASGWVTGSAP
                     IAISPSTFESAGFPYTYDIVFTDDDSAYVCDINRFNYVYNLEGNTGSSSAPFKYLTKF
                     ALPFYVVNRDVRDKNGEPERLGLLVEDLNRNGAFDIFEDRFLAGHRTERPTIMGTLNV
                     YWAGTVFGFDFHQVMSEEELPNPDDIYRVKFRRPFSTADSVLFTVAGETAVTAAALDE
                     AMARIRVVPNPYVVTNTMEPAVANKYLNQRRRIMFTHLPARCTIRIFTSSGILVDTIE
                     VENPPSDGTVHWDLLTREGLEIAAGMYVYHVKAEVTGKEKIGKFAVIK"
     gene            6584..7636
                     /locus_tag="PKM23_08810"
     CDS             6584..7636
                     /locus_tag="PKM23_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY91612.1"
                     /translation="MKKKILYLILMMAPAWAGAQNKPYRVGTTAANFLEMGVGSAANA
                     MGEAYVSQARDLSSIYWNPAGLAYLERSEVLCMYHPWIAGINVSFAGTALLLPRIGAV
                     GLSITSMNYGRTEVTTMSMQEGTGETYGANEFAVSLSYSRKLADWFAFGATGKYIASN
                     IWHLGASAMAMDLGVMVNTHFFSFSGKREDGMTIGMSIANYGTKMAYDGMDLLRPIDI
                     EPNENGNYKDVEGQFRVQGWDLPLIFRVGVSIVPIAAKNQRLVLAVDALHPNNNNESL
                     NLGAEYSYRLIGVGKFFLRGGYRGLYLDQSEYGPTFGAGVALTLMHNKLLRFDYAFKD
                     VGILGNTSTYTLAFTF"
BASE COUNT         1618 a         2568 c         2119 g         1544 t
ORIGIN      
        1 acctggcctc ttcttgtgtc gtgctgaaga acctggtggg caccagaggg gggctctcgc
       61 tcctacccgg cctgcttttc ttgttccgcc tcgccctgta cagaaaattc attccggtta
      121 gttttttttc ttgcaaaacc aactaaaaat gactagacta tgccatcctt tccttttcat
      181 ccacgcggcc gcccttctgg ccgtcttcgt tccatgatca tctatatcgc ggagtgacca
      241 tgattcgatc cgcttactgg ctgattctcc tcctcctgtg cgcaacgctc gccattcccg
      301 ccctgctcac ggcccagacc accggcaaaa tcgccggcac ggtcaaggat gccgccagcg
      361 gccagcccct gcccggggcc gtagtggtca ttgagggcag caagctgggc gcggccgctg
      421 atattgaggg ctcctttttc atcctcaacc tgtcgccggg ggtctatacc ctgcaaatac
      481 gcatggtcgg ttacgagtcg atgagcatcc gccaggtgcg cgtctcggtc aaccgcacca
      541 gcgagatcac tgcccgcctc aaggccacgg tgatcaaggg gcaggaggtg atcgtcaccg
      601 ccgacgccat cgaggtcaag aaggaccaga ccagctcggt gcgcaacgtc tcggccgaca
      661 agatcgaaaa attgccggtc gagagcctga gcgccgtgat caacatgcag gccggcgtgg
      721 tggctggcca tttccgcggc ggccgcagca ccgaggtctc ttatctgatc gacggggttc
      781 aggtcgatga gggtttcagc ggcagcgggc gcgccgtcag cctcgaggcc gaggcgatcg
      841 cagatctcga ggtgatcacc ggtaccttca acgccgaata cggccgcgcc atgagcggcg
      901 tggtcaatgc ggtgaccaag gagggaggca accagtttca tggcagcttt tcgagcagtc
      961 tcgccaacta cctgacgccg catgatgaca tcttcatcgg cctcaacagc ggccgccccg
     1021 gcctcaacct cagccaggat tacaaattcc agctcgaggg tcccttcttc agggacaagc
     1081 tctccttttt catcaatggc cgctaccagg atcagaacgg ctacctcaat ggcatccgcc
     1141 gcttcaaccc ctgggattac agcgactatt ccgctgagga tccggccgaa tggcatatcg
     1201 aacacaccgg cgacaacaag tacgtctcca tggaccattc gcaaaacctt tcgctgatga
     1261 ccaagctggc ctggaacgcc acctccagga tcaaggtcac cgggctctac accctcaacg
     1321 acgatatggg ccagggctac agccatggtt ataaatacaa tcccgacgcg cgcggcttct
     1381 ccttccacac cgcccatatg ggggcgctgc agctcaacca tatgctctcc caaaagttct
     1441 tctacgaggt caagctgtcg catgttgaca accgctacaa atcgtaccgc tacgagaatc
     1501 ccctcgactc ccgttttctg cacggcaagt actacggctc cggccacagc ggattcgcca
     1561 ccggcggcat cgacggtccc ggccgcagca tgtcgttcta caaggacagc aacctcaagc
     1621 tcgatgccac ctggcaggcg accaaacacc atgccttcaa ggcgggactg cacggaatct
     1681 atcacgacat cgaccaggac cgcatcgatg tgcgcaataa atggtccggc acggccgagg
     1741 agaatcttcc ggtcacggat gccaacggca agatcacttt tccttattac gaactcgaga
     1801 tcgtccccca tacccccaaa accatgggca tctataaaaa acagccctac gagttctccg
     1861 gctacctgca ggacaagctc gaatacaatg agatggtggt caatatcggc ctgcgctatg
     1921 attatttcaa ccccgaccag atctatccga ccaaccggcg caatccctcc aaccagctca
     1981 atctgcccga ttcgatgttg acctcctaca agcgcgctga accgcaaacc cagctctcgc
     2041 cccggctcgg tctcgcctat cagctcagcg atcaggcggt gctccatttc agctacggcc
     2101 attttttcca gatgccgccg atgtacgccc tctatgagaa caacatcttc cgtgtgccgg
     2161 tgggggatta tggcaccacc atgggcaatg cccagctcaa cgcgcaaaag acgatctcct
     2221 acgagatcgg tgtctggcag gagctgacca aagggatggg gctggaactg gcgctctttt
     2281 acaaggatat ctacgatctg ctcagcacga tggtcatcac cacctataac cagatcgagt
     2341 atgggctcta taccaacaag gattatggca acgcccgcgg cctggaggtc aagtacgatt
     2401 tcaccatggg gcggctctcg gccaacctca actataccct gcagtacacc aagggcaacg
     2461 ctgacaatcc ccagcagact ttcacacgcg ccggctcgag catggatccc attcgcaggc
     2521 tgatcccgat gtcatgggac cagcgccata ccttcaatgc cacagtcaat tacagcgacc
     2581 cacactacgg cgccaccctg accggttatt acaattcggg cacgccctac tcgttcagtc
     2641 ccatgaccga gagcccgctc tcgctgatca acctgtacca gaacaacgac tacaagccgg
     2701 ccggctatac cctcgacatg acctcctatt atgatattac cgtttggggg aagcagaagg
     2761 tgcggctgac cctgtcggtc tataatctgc tcgaccggct caatgccaac tgggtctaca
     2821 gcgataccgg ccaaccctac acgacgatcg tgcgtgaggc gcagctggcg acccaccgca
     2881 gcgatttcaa tgattattat gaccgggtca agaatcctac ggcctactcc tcgccccgcc
     2941 aggtcaagct ggggatggga tttgtgttct agacggataa caggagccag atgatgcgcc
     3001 tagtttgcac ggttttgttt acgctgatac tgctttccac acccctcttc ggccaggggc
     3061 gcccttacga aggccctgag gatcccgccg gcgacatcgc cgctatccgc attggctatc
     3121 tcaacggcaa ccgcatcttt ctcgctttcg agaacaacac ggcctttgcg gtgggcggca
     3181 agccgatttc gagcaagtgg cccaataact atgaggggac cgtgatgctc gaccgcgtct
     3241 cactgttgat cggcgccgag gtctttgtcc gccaggattc catcccggtg accgatctgg
     3301 cggaggtggc gcggctgcgg gccgagatcg ataccctctt cttcatccag tccaacgacc
     3361 ccggccgcaa cgatcagaac tacgatgcca ccatcgattg ggcgctctac ccggtgccgg
     3421 gctatttcaa cgagacccag gattttgtcg ccatgagcaa ttttcccacc tcctggccgc
     3481 cgcagggctg gcccgcgccc aattttgcca ccaagtggcc gggcgagtgg aatggccgct
     3541 tcggccgcgg catcaggtat gcggatctcg aaacctactt cgtcgctaat gatgcgcagg
     3601 acctcgagaa catcgtccgg cgcaacgatc ccgatcaaaa gctcatcaag acagggccgc
     3661 gttactatcc ccggcctggc caccgcatcg gcgatatcaa tcccgacgtc accgtgcagc
     3721 gcgggatgcc ctggggcgga ctcgggctgc gcgtctccct gcgcggcttt cagtggaaca
     3781 atcccgaggc gcgcgacatc atcttccttg aatacgagat ctccaacatc tccgcctatg
     3841 acttgccgtc gtgcggcttt ggctttttcc tcgacacggc catcggcggc gagcacagcc
     3901 ccgacggcga tgtcggctat tacaatacca aactcgacct ggcctactgc tgggactatg
     3961 acggggtcgg cgccggcggg ctcagccccg gcatcctcgg catggcctac ctcgagagcc
     4021 ccggcaagcc cttcgacagc cgcgacaacg acgaggatgg cctgatcgac gaaaaacgcg
     4081 ataatcccgc cggggagaag gtcggtcctt acgacggtat cgccaatctg gccagtttcc
     4141 tggagttcta caacctgcag gaatcggatc tcaaggagca ttttcagggg gacgaggacg
     4201 gggactgggt ggacggcgtc gacctcaacg gcaacggcgc ctacgcttat caggacgccc
     4261 tcggcgactg gtatctggaa cccggcgaga atcccggcaa tgatgtcggc ctcgacgggg
     4321 tcggaccgat ggatatcaac taccacgggc cggatgaggg ggagggcaac caccgccctg
     4381 atttcgtcga aggcgtgggc tgcgaaccca acttcgccgc caccgacgtc agcgaatcgg
     4441 atatggtcgg cctcacctcc ttccacctct tcgattatta ccagtggacc ggaaacaact
     4501 ggctggtcaa ggatgacaag aatgtctggg acctgatgaa cgatcccgac tttcgcgcct
     4561 acattggcga gcccaacagc atctatttcc agttctcgac cagcaagttt cccttctata
     4621 aaggccgcac cgagcgcatc tccatcgcca tgctccacac ctacgagaac ctcgccggcc
     4681 tccagtccac cgcccattcg gcacccacgc tcttccgcgc aaaggagacc gcccagatca
     4741 tctacgagaa ggattaccgc ttcgcccagc cgcccctgct gcccagcctc aaggccaccg
     4801 cgggcgacgg caaggtgatc ctcacctggg acgaccgcgc cgaccggctg acccgcgagc
     4861 ctttcctcga gaatatcaac gactttgagg gctacaagct ctaccgcgcc accgataagt
     4921 tgatgtccga tcccgaggtg gtcaccgacg gccagggcac ccgaatgttc cgcaagccga
     4981 tctatcagtg cgacctgctg gacaatatct cgggttatgc cgatttcggc ctcgtcggcg
     5041 gcacctcctt ctacctcggc gatgagaccg gactgaccca ctacttcgtc gacgaaaatg
     5101 tcctcaatgg ccgtacctac tattatgccc ttgtggccta cgatttcggc gcgcgcgagg
     5161 tcggcggcgg catctcacct accgaaaaca acattgtcat cgagctggat gaggccgaag
     5221 agatcatccg catgggcaac aatatcgccg tggttacccc gcaccagaac gcagcggggt
     5281 atgatgatcc cacggtcgcc ggcgaggcgg ctgagacctt cggcagcggt ctggtcgaac
     5341 ccctgatcgt tgatcgtgcg gcgatcaagc cgggccatac ttacaaactc tttttccgca
     5401 cagacactgt cggctacgcc cgccgcaaca gcggcatgcg cagcagtcac gatttgatcc
     5461 gctgcaacag cggactccag gtctacgacg tcagcgacgg caaccgcctc gtctataagg
     5521 agagtccggc gcgcttcccg gcggccaaca tccagcctga aggcaaggtt ttgaccctcg
     5581 acggcacggt ggaggagacc ttcccctgct atgagcatac ccgcgagctc ttcaccgatg
     5641 ttttcgacgg gctgcagctc aaactctcga atctgaccgt caccggcgaa tacgatgcgg
     5701 ccgcctcggg ctgggtgacc ggcagcgccc ccatcgccat cagccccagc actttcgaat
     5761 cggcgggctt tccctatacc tatgatatcg tttttacaga tgatgattcc gcctatgtct
     5821 gcgacatcaa ccgctttaac tatgtctata atctggaggg caacaccggc tcctcgtcgg
     5881 cgccctttaa atatctcacc aaattcgccc tgccttttta cgtcgtcaac cgcgatgtgc
     5941 gcgacaagaa cggcgagccc gagcggttgg ggctgctggt ggaggacctc aaccgcaacg
     6001 gcgcctttga tatcttcgag gaccgctttc tggccgggca ccggaccgaa cgcccgacca
     6061 tcatggggac gctgaatgtc tactgggccg ggacggtctt cggctttgat tttcatcagg
     6121 tgatgtctga agaggagctg cccaatccgg atgatatcta ccgggtgaaa ttcaggcgtc
     6181 ccttttcaac cgctgattca gtactcttca cggtggcggg ggagaccgcg gtgacggcgg
     6241 ccgctctcga tgaggccatg gcgcggatcc gcgtggtgcc caatccctat gtggtgacca
     6301 acaccatgga gccggccgtg gccaacaagt atctcaacca gcgccgtcgg attatgttca
     6361 cccatctgcc ggcgcgctgt acgatccgca tcttcacctc cagcggcatt ctcgtcgaca
     6421 ccatcgaggt cgagaatccc ccaagcgacg gcacggtgca ctgggatctg ctcacccgcg
     6481 aggggctgga gatcgcggcg gggatgtatg tctatcacgt caaggcagag gtgacgggca
     6541 aggaaaagat cggcaaattc gcggtgatca aatagaggat cccatgaaaa agaaaattct
     6601 atatctgatt ctcatgatgg cgccggcatg ggccggtgcg cagaacaaac cctaccgcgt
     6661 cgggaccacc gccgccaact ttctcgagat gggggtcggc agcgcggcca acgccatggg
     6721 cgaggcctac gtcagccagg cccgcgacct ctcctccatc tactggaatc cggcggggct
     6781 ggcatacctc gagcgcagcg aggtgctctg catgtaccat ccctggatcg ccggtatcaa
     6841 cgtcagcttt gccggtacgg ccctgctgct gccgcgcatc ggcgctgtcg gactctccat
     6901 caccagtatg aactatggcc gcaccgaggt caccaccatg tcaatgcagg aggggacggg
     6961 cgagacctac ggcgccaatg agtttgcggt ctcgctctcc tacagccgca agctcgccga
     7021 ctggttcgcc tttggagcga ccggcaagta catcgcctcc aatatctggc acctcggggc
     7081 ttcggccatg gccatggatc tcggcgtcat ggtcaacacc cactttttct ccttcagcgg
     7141 caagcgcgag gacggcatga ccatcggcat gagtatcgcc aattacggca ccaaaatggc
     7201 ctacgacggc atggatctgc tgcggccgat cgacatcgaa cccaatgaga acggcaacta
     7261 taaggatgtc gaggggcaat tccgcgttca gggatgggac ctgccgctga tcttccgggt
     7321 cggagtctcg atcgtgccca tcgccgccaa aaaccagcgg ctggtgctgg ccgtggatgc
     7381 tctgcacccc aataacaaca atgaatccct caacctgggc gccgagtact cgtacaggct
     7441 gatcggcgtc ggcaaatttt tcctgcgcgg cggctatcgc gggctctatc tggaccagag
     7501 cgagtatggt ccgacctttg gcgccggcgt cgctctcacg ctcatgcata acaagctctt
     7561 gcgcttcgac tatgccttca aggatgtcgg catcctcggc aatacctcca cctacaccct
     7621 ggccttcacc ttctgaactg actcccctgc cggccgggtg cgaaccctcc aggttgcgcc
     7681 cggtcgcctc cgcattctcg ccactcccgc tcgctctcgt ttgcgtctcg cccacccggc
     7741 ctggtacaaa tctcgttctc agccggctcc cggctggcgc gcaggcgggc cccccatcca
     7801 tctcgttccc acctggctcc cggctggcgc gaaagcccgg ctgggcacc
//
LOCUS       DAOPWU010000152         7824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9286_length_7824_cov_5.743210, whole genome shotgun sequence.
ACCESSION   DAOPWU010000152 DAOPWU010000000
VERSION     DAOPWU010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9286_length_7824_cov_5.743210"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKM23_08815"
     CDS             <1..270
                     /locus_tag="PKM23_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY91613.1"
                     /translation="ALSANYPNPFNPTTTISYDLPAKSHVRLTVFNAMGQIVSRLVDK
                     EQTAGSYRVSWNGTNIDGSAAPSGIYFYRLEGDGFAKTMKMTLMK"
     gene            489..3530
                     /locus_tag="PKM23_08820"
     CDS             489..3530
                     /locus_tag="PKM23_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91614.1"
                     /translation="MLRMSKWTLFLLPVLAFGLFAGTTGKLTGVVTDAETGDPLPGVN
                     VVIEGTSYGAITNLEGRYTIINLQPGTYDLRFSMIGYATFRVRQVKVEIDRTTETNQA
                     IKSQTIEGESVVVVAQRPVVKRDISSSQVNIEAKSVETMPVQTVRQVLVLQAGIQQGS
                     EGILVRGGSANQTVMMLDGLSLNDERSNIPYAAISVSAVKEIQIQTGGFNAEYGNVRS
                     GLVNVITKEGDARRYNFAATLNYSPASAKHFGTSLYDPMSFFNRVYMDPAVCYTGTDN
                     GAWDDYTRSQYPNFDGWQAVADKTLQNADPSDDLTAEGAKRLYEWQHRRQGDIKKPDY
                     VVDLGFGGPVPLLSQFGGTRFFISHFREQEMFIFPLSRDGYNENYTQLKLTTDLSRNM
                     KLMYTGLYGEVYSASPYNWTTTPTGRVLRDQSEIADLLNSTDGGAILYVPGYYSPSAI
                     YRQVHGLKLTHMLSPKTFYEVSLEYQNNRNNTYEMTLRDTSKVYEIVNGYYVDEMPYG
                     YWGYGTNSINGDRMGGWMNLGRDKSVNSTTSFNANLTSQVTSRHQIKTGAQVVYNDYD
                     VNSGTVSPSNNFWNRSMTYNVFPFRVGAFLQDKIEYEGFVANIGVRMDHTNPNTKYMD
                     VEAYDVNLGAGYGQEITSTVEKVATTARTYWSPRLGISHPITNNSKLYFNYGHFRSEP
                     YSSYRFRLQQESNGLVTYLGNPNMEMEKTVSYELGYSQSLFNTMLLNVAAHYKDVSNQ
                     AGWVYYHNFKGTVDYYKASNNNYADIRGFEVTLNKQSGDWITGFVNYTYEVSTTGYFG
                     LIRNYENPNQQRAYLRENLYQERPHPRPYARANVDLHTPKGFGPAVAGFRPLESINVS
                     LLGDWRAGGYVTYNPNNIPGVADNVRWKDWFNLDLRLSKALRIAGVESQFYLDFSNVL
                     NIKYLYYASFADNYDYIDYLESLNLDWEKGDEKGSDKIGELRPEDVKYDPLERNPYND
                     PEITKRNDKRKESKSYIDNPNIDSLWWLHPRDITFGIRINF"
     gene            3548..>4275
                     /locus_tag="PKM23_08825"
     CDS             3548..>4275
                     /locus_tag="PKM23_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin"
                     /protein_id="HNY91615.1"
                     /translation="MRRTLLPPMPALQGIVLALMLSGIWFGVLAQVVSTDRRYIRIGE
                     LQNHYTAYGSDRAWNNTWYEGLQWPAGYFYQDNSVIRRQWIGCEDFTSARDEHYNHFA
                     ITFSEGNVSTALFPVELKQTARFAIPTVYVDGVDVSAPYAADADEIDESIIPDRIVVN
                     KVNSLMGLTITRTIYAFSQQYHNNYFIQTWKLENTGNVDYDDEIELNRTLKGVRFSFS
                     VRYAVSREGANAIGGSQTWGKWSWV"
     assembly_gap    4276..4375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4376..5614
                     /locus_tag="PKM23_08830"
     CDS             <4376..5614
                     /locus_tag="PKM23_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin"
                     /protein_id="HNY91616.1"
                     /translation="DDPAQPNTLGWHAGDTYPGVSSYTPDGAASMDILYSVISGNAYM
                     GLGGTNRFDETYMATNPDPSTVHNDGGGTNVWVCYGPWDLQPGESVTIVEAEGISGLN
                     RKQCETIGARWKKAYLDPGDKGPFTLPNGTTTDNKDTYKNQWVFTGKDSILMTFGRAK
                     RALDANLQIPQPPLPPTVVDVKSGGDRITVSWTASPSENDPDFAGYKVFRAVGRTDTV
                     FQEIYNGGREPRAFDDVTATRGISYYYYVVAYNDGSRNNTGECNPTGPLMSGRYYTQT
                     SEPAYLRRQAGETLADQLDANGNIVKPGIRIVPNPYDIKSRKLQYIGEDDKIMFLNIP
                     GHCTIRIYTERGDLINQIEHEDGSGDESWNSITSARQVVVSGIYIVHFEVTQDQYESG
                     TNKLIHKKGDTAVRKLVIIR"
     gene            5704..>7824
                     /locus_tag="PKM23_08835"
     CDS             5704..>7824
                     /locus_tag="PKM23_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNY91617.1"
                     /translation="MKKIILTGLLLLSFTALLAADNTEFRATWVITWNIYNGHQPVET
                     LKARIRKILDDHQKANMNAVLWQVRQGGTAYYNSSFEPWGPYLDNQDPGFDPLAFAIE
                     EAHSRGMELHAWFNAFNVSSSTPDAAVYKHPEWVCRNSSGQSMSSDRCFSPGLAAVRD
                     YTVAVAMELVRNYDIDGLHLDYVRWNEYSGAASLHKESDRPRLDGMISQEEIELLNAN
                     MASRFLYDVDHPYNAGVPAGFSAWEEWWRWSVTEFVRTLHDSVQAAKPWVRLSAAALG
                     NYNWGGWQGYGSVFQDAALWFNQGYVDQLTPMHYHWTTGAGFYGMLTGTSASWGPWIQ
                     QGIAAGRLFSVGPPSYILSEDKIMDRHASIVDYCRMVPWVDGFQFFSYGTWESNQFFG
                     ETGATFFPNKSKIRAAKYLSDALPSSPALVVNRVDSLTYEITITPPAGQTAPGRMALY
                     RIEQGDPRPDLDPILQICWSGSPVTFIDRFDGLQNHAGSFFYYATAFDRFWNESLPSP
                     LAEAGPVPSFAPQVVATTPAPGETVAVNHDLTVVFSKAMDSTSVAAALAFDPAVLINR
                     MRWSEGYTLLSLEPAEPLRFGTTYTLTISEAAADVNGKALDGDADGIPGGIFKIQFAT
                     DARDISAPELLAFYPTVDDLLPVEGVINLLFDERIAPASITDSTFLLTRAGEPVQAMW
                     AHVPAGSRSVLSIQPAKPLEVSTAF"
BASE COUNT         1645 a         2549 c         2047 g         1483 t
ORIGIN      
        1 gcgctgagcg ccaactatcc caaccccttc aacccgacca ccacgatcag ctatgatctg
       61 cccgccaaga gccatgtccg gctcacggtc ttcaatgcca tgggccagat cgtctccagg
      121 ctggtcgaca aggagcagac ggctggcagc tatcgcgtct cctggaacgg caccaacatc
      181 gacggctccg cggccccgag cggcatctat ttctaccgcc tcgaaggcga cggattcgcc
      241 aaaaccatga agatgaccct gatgaagtag tctctacccc gagaaagcct gtgcaccgca
      301 aggtgcacag gcttttctca tcgcctgccc gaagaagaat gcagagcgcg ggcaagctgt
      361 gcacaggggc tggtgcgcca gccctcatgc agagtttgtc cacccgacca cggcatgtta
      421 ttgtcaggtc actctggaat cctatacgga caatcgttgc ggctctacag tcaacaggag
      481 agatttttat gctgagaatg tcaaaatgga ccctgttcct gctgccggtg ctcgcgttcg
      541 ggctcttcgc cgggacgacc ggcaagctga ccggcgtggt gaccgacgcc gagaccggtg
      601 atcccctgcc cggcgtgaat gtcgtcatcg agggcacctc ctacggtgcc atcaccaatc
      661 tcgagggacg ctacacgatc atcaacctgc aaccgggcac ctatgacctg cgcttttcga
      721 tgatcggtta tgccaccttt cgggtccgcc aggtgaaggt ggagatcgac cgcaccaccg
      781 agaccaatca ggccatcaaa agccagacca tcgagggcga gagcgtggtc gtcgtcgccc
      841 agcgcccggt ggtcaagcgc gatatctcca gcagccaggt gaacatcgag gccaagtcgg
      901 tcgagaccat gccggtccag acggtgcgcc aggtcctcgt tctccaggcg ggcatccagc
      961 agggcagcga gggcatcctc gtccgcggcg gcagcgccaa ccagaccgtg atgatgctgg
     1021 acggcctctc tcttaatgac gagcgctcca acattcctta cgccgccatc tccgttagcg
     1081 cagtcaagga gatccagatt cagaccggcg gtttcaacgc cgaatatggc aacgtccgtt
     1141 ccggcctcgt caatgtcatc accaaggagg gcgatgccag gcgctataac tttgccgcca
     1201 ccctgaacta cagcccggcc tcggcaaagc atttcggcac ctccctttac gatccgatgt
     1261 ccttcttcaa ccgcgtctat atggatccgg cggtctgcta caccggcacc gacaacggcg
     1321 cctgggacga ctatacccgc agccaatacc ccaatttcga cggctggcag gcggtggccg
     1381 acaagaccct gcagaatgca gacccgagcg atgacctcac tgcggagggg gccaagcggc
     1441 tctatgaatg gcagcaccgc cgccagggcg acatcaagaa acccgactat gttgtcgacc
     1501 tcggcttcgg tggccctgtg cccctgctct cccaattcgg cggcacccgc ttcttcatca
     1561 gccacttccg cgaacaggag atgttcatct ttccgctctc gcgggacggc tacaacgaga
     1621 attacaccca gttgaagctg accactgacc tctccagaaa tatgaaactg atgtacaccg
     1681 gcctttatgg cgaagtctat tcggcctccc cctacaactg gaccaccacg ccgaccggcc
     1741 gcgtgctgcg cgaccagtcg gagatcgcag atcttctcaa cagcaccgac ggcggcgcca
     1801 tcctttacgt gcccggttat tacagcccga gcgcgatcta ccgtcaggtg cacggcctca
     1861 agctgactca catgctctca cccaagacct tttacgaggt cagcctcgag tatcagaaca
     1921 accgcaataa tacctacgag atgaccctgc gcgataccag caaggtatac gagatcgtca
     1981 atggttacta tgtcgacgag atgccctacg ggtactgggg ctacggcacc aacagcatca
     2041 acggcgaccg catgggcggc tggatgaatc tggggcggga caagagcgtc aactcgacga
     2101 ccagcttcaa cgccaacctg accagccagg tcacctcgcg ccatcagatc aagaccggcg
     2161 ctcaggtggt ctacaacgac tatgacgtca attcgggcac agtcagcccc tccaataatt
     2221 tctggaaccg gtcgatgacc tacaacgtct tccccttccg cgtcggggcc ttcctccagg
     2281 acaagatcga atacgaaggc tttgtcgcca acatcggcgt gcgcatggac cacaccaatc
     2341 ccaataccaa gtatatggat gtcgaagcct atgacgtcaa cctcggcgcc ggttatggcc
     2401 aggagatcac cagcacggtg gaaaaggtgg cgaccacggc caggacctac tggagtcccc
     2461 gtctcggtat ctcccatccg atcaccaaca acagcaagct ctatttcaac tacggccatt
     2521 tccgttcaga gccctactcc tcctatcgct tccgcctgca gcaggagagc aacggcctag
     2581 tcacctatct cggcaatccc aacatggaga tggaaaagac cgtctcctat gagctgggct
     2641 attcgcagag cctcttcaat accatgctgc tcaatgtcgc cgctcattac aaggatgtca
     2701 gcaaccaggc cggctgggtc tattaccata acttcaaggg aacggtcgat tactacaagg
     2761 cgagcaataa taactatgcc gatatccgcg gttttgaggt gaccctcaac aagcagagcg
     2821 gcgactggat caccggcttt gtcaactata cctacgaggt gagcacgacc ggctacttcg
     2881 gcctgatccg caactatgag aatcccaacc agcagcgcgc ctacctgcgc gagaacctct
     2941 atcaggagcg gccccatccg cgtccctatg cccgcgccaa tgtcgatctc cataccccca
     3001 agggcttcgg tcccgccgtc gccggcttcc gtcccctcga atcgatcaat gtcagcctgc
     3061 tcggcgactg gcgcgccggc ggctatgtca cctacaatcc gaacaacatc cccggcgtgg
     3121 ccgacaatgt gcgctggaag gactggttca acctcgacct gcgtctctcc aaggccctgc
     3181 gcatcgccgg ggtggaaagc cagttctatc tcgatttctc gaacgtcctc aacattaaat
     3241 atctctacta cgccagcttt gccgataatt atgactatat cgactatctt gagtcgctca
     3301 atcttgattg ggagaaaggg gacgagaagg ggagcgacaa gatcggcgaa ctgcgtcccg
     3361 aggatgtcaa gtatgatccc ctggagcgta atccctacaa tgatccggag atcaccaaac
     3421 gcaacgacaa gcgcaaggag agcaagtcct atatcgacaa tcccaacatc gattcgctct
     3481 ggtggctcca tccgcgcgac atcaccttcg gcatcaggat caacttttaa ttgcatgagg
     3541 agataaaatg agaagaaccc tacttccccc aatgcccgct ctgcagggga tcgtcctcgc
     3601 cttgatgctt tcggggattt ggtttggtgt gcttgcgcag gtggtttcga cggaccggcg
     3661 ctatattcgc atcggtgagc tgcagaatca ttatacggcc tatggttccg accgcgcctg
     3721 gaacaacacc tggtacgagg ggctgcagtg gccggccggc tatttttatc aggacaattc
     3781 ggtcatccgc cgccagtgga tcggctgcga ggatttcacc agcgcgcgtg atgaacatta
     3841 caaccacttt gccatcacct tttcggaggg gaacgtcagc accgccctct tcccggtcga
     3901 gctgaagcag accgccaggt tcgccatccc caccgtctat gtcgacggcg tcgatgtcag
     3961 cgccccctac gccgcggatg cggatgagat tgacgagagc atcattcccg accgcatcgt
     4021 ggtcaacaag gtcaacagtc tgatgggatt gaccatcacc cgtaccatct acgccttcag
     4081 ccagcagtat cataacaact atttcatcca gacctggaag ctggaaaata ccggcaatgt
     4141 cgactatgat gatgagatcg aactgaatcg caccctcaag ggggttcgct tcagcttcag
     4201 tgtccgctat gccgtgagcc gcgaaggtgc caacgccatc ggcggcagcc agacctgggg
     4261 caagtggagc tgggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatga
     4381 tccggcccag cccaataccc tcggctggca tgcgggcgac acctatccgg gagtgagcag
     4441 ctataccccg gacggcgccg cctcgatgga tatcctctac agcgtgatca gcggcaatgc
     4501 ctatatggga ctgggtggca ccaaccgctt cgatgaaacc tacatggcca ccaatcccga
     4561 tcccagcacc gtgcacaacg acggcggcgg caccaatgtc tgggtctgct atgggccctg
     4621 ggaccttcag cccggcgaat cagtcaccat cgtcgaggcc gagggcattt ccggcctcaa
     4681 ccgcaagcag tgcgagacca tcggcgcccg ctggaaaaaa gcctatctcg atcccggcga
     4741 caaggggccc ttcaccctgc cgaatggaac cacgacggac aacaaggata cctataaaaa
     4801 ccagtgggtc ttcaccggca aggattccat attgatgacc ttcggccgcg ccaagcgcgc
     4861 cctcgatgcc aacctgcaaa tcccgcagcc cccgctgccc cccacggtgg tggacgtcaa
     4921 gtcgggcggc gaccgcatca ccgtctcctg gaccgccagc ccttcggaga acgaccccga
     4981 ttttgccggc tacaaggtct tccgcgccgt cggccgcacc gacaccgtct ttcaggagat
     5041 ctacaacggc ggccgcgaac cccgcgcttt tgatgatgtc accgccacgc gcggcatctc
     5101 ctactattac tatgtagtcg cctataacga cggcagccgc aacaataccg gagaatgcaa
     5161 tcccaccggc cctctgatga gcggccgtta ctatacccaa accagcgagc cggcctacct
     5221 ccgccgccag gctggcgaga ccctggccga ccagcttgat gccaatggca acattgtcaa
     5281 gcctggcatc cgcatcgtgc ccaatcccta cgatatcaag tcgcgcaagc tgcagtacat
     5341 cggcgaggat gacaagatca tgttcctcaa catccccggc cactgcacca tacgcatcta
     5401 taccgagcgc ggcgacctga tcaaccagat cgaacacgaa gacggcagcg gtgacgagag
     5461 ctggaactcc atcaccagcg cccgccaggt ggttgtcagc ggcatctata tcgtccactt
     5521 tgaggtgacc caggatcagt atgaatccgg caccaacaag ctgatccata aaaagggcga
     5581 caccgccgtg cgcaagcttg tcatcatccg ctaaacttta taagagtaaa aaccgctgcg
     5641 ctatcatcgc cgccgggcgg ccgcagcggt tttttttacc ccgaaccacc aacgaggtca
     5701 cgcatgaaaa aaatcatact gacagggctg ttgctgctct cgttcaccgc tcttctcgct
     5761 gcggataaca ccgaattccg cgccacctgg gtcatcacct ggaatatcta caacggtcac
     5821 cagccggtag agaccctcaa ggcgcgcatc cgcaagatcc tcgatgacca tcaaaaggcc
     5881 aatatgaacg ccgtgctctg gcaggtgcgc caggggggga cggcctatta caattccagt
     5941 tttgaaccct ggggtcctta tctcgacaac caggatcctg gcttcgatcc cctcgccttc
     6001 gccatcgagg aggcccacag ccgcggcatg gagctgcacg cctggttcaa cgcctttaat
     6061 gtgagctcaa gcacgcccga cgcagcggtc tacaaacatc ccgagtgggt ctgccgcaac
     6121 agcagcggcc agagcatgag ttcagaccgc tgcttctcgc cgggactcgc cgccgtgcgc
     6181 gactataccg tcgcagtggc gatggaactt gtgcgcaatt atgatatcga cggtctgcac
     6241 ctggattacg tgcgctggaa cgagtactcg ggtgcggctt ccctgcacaa ggagagcgac
     6301 cgcccgcggc tcgacggcat gatttcgcag gaggagatcg agctcctcaa cgccaatatg
     6361 gcctcgcgct tcctctatga cgtcgaccat ccctacaacg ccggtgtgcc cgccggcttc
     6421 agcgcctggg aggagtggtg gcgctggtcg gtcaccgagt ttgtccgcac cctgcacgac
     6481 tccgttcagg ccgccaagcc ctgggtccgc ctttcggccg ccgccctggg caattacaac
     6541 tggggcggct ggcagggcta cggctcggtc tttcaggacg ccgccctctg gttcaatcag
     6601 ggctatgtcg atcagctgac gccgatgcat taccactgga ccaccggggc cggtttttac
     6661 ggcatgctga ccgggacttc cgccagctgg ggtccctgga ttcagcaggg catcgccgcc
     6721 ggccgcctct tcagcgtcgg tccgccctcc tacattctca gcgaagacaa gatcatggat
     6781 cgccacgcct ccatcgtcga ttactgccgc atggtgccct gggtggatgg attccagttc
     6841 ttcagttatg gcacctggga gtccaaccag ttctttggcg agaccggggc gacctttttc
     6901 cccaacaagt ccaagatccg cgccgccaaa tatctctctg acgctctgcc ctcctcgccc
     6961 gctctcgtgg tcaaccgcgt tgactcgctg acctatgaaa tcaccatcac cccgccagcg
     7021 gggcagaccg cccccgggcg catggccctc taccgcatcg agcagggcga tccccggccc
     7081 gatctggatc ccatcctgca gatttgctgg agcggaagcc cggtcacctt catcgaccgc
     7141 tttgacggcc tccagaatca tgcgggctcc ttcttttact atgccaccgc cttcgaccgc
     7201 ttctggaacg aatccctgcc ctcgcccctt gccgaggccg gcccggtgcc ttccttcgca
     7261 ccgcaggtgg tcgccaccac ccccgctcct ggggaaaccg tcgcggtcaa ccatgacctg
     7321 accgtcgttt tcagcaaggc catggattct accagcgtgg cggcggcgct tgccttcgat
     7381 cccgccgtcc tcatcaaccg gatgcgctgg agtgaaggct ataccctgct ctccctggaa
     7441 ccggccgagc ctctgcgctt cggcaccacc tacaccctca ccatcagcga agcggccgcc
     7501 gatgtcaacg gcaaggccct ggacggcgat gctgacggta ttccaggcgg gatttttaag
     7561 atccagtttg cgaccgacgc ccgggatatc agcgctcccg aactgctcgc cttttatccc
     7621 acagtcgatg atctgctgcc ggtggaaggg gtgatcaatc tgctcttcga tgaacgcatt
     7681 gccccggcct cgattaccga ctcgactttc ctgctgaccc gcgcaggaga gccggtgcag
     7741 gcgatgtggg cccatgtccc tgccggcagc cgttcggttc tttcgatcca gcccgccaaa
     7801 cccctggagg tgagtacagc cttc
//
LOCUS       DAOPWU010000153         7820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9294_length_7820_cov_4.528783, whole genome shotgun sequence.
ACCESSION   DAOPWU010000153 DAOPWU010000000
VERSION     DAOPWU010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9294_length_7820_cov_4.528783"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3491
                     /locus_tag="PKM23_08840"
     CDS             <1..3491
                     /locus_tag="PKM23_08840"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNY91618.1"
                     /translation="LPMSGVTVRLHDPAGALLAQQVTAADGAFLFDRLAPGSYFLSFV
                     PPAGYALTLFTADTTSMVNSDADMSTSRTILTVLGQGENDLTWGAGFYELSSLGDYVW
                     HDVNMNGLQELGESPVEDVAVTLLNDDGLPVDSTRTDADGFYYFPNLPPGGYALRFTL
                     PAHYIFTGTDQGGNDTRDSDADRTNGRTTDISLGLAVHDSTWDAGLIKLSSVGDYVWE
                     DTDADGVQDGDELGLPGIRIVLTDTLGQPVAETVTDENGNYLFDDVVPGCYIVRVDET
                     TLPRSYVTSTFNNPMTVCLAPDEHRRDADFGYRSNYGSIGDFVWFDINRDGLQDPGEP
                     GIPNVLLRLEEINTGETAKQLTNSEGKYLFRTLLPGEYRVYVDVSTLPEGYFLTSGRS
                     EFIISLGDQENYREADFGYWREDAGCDKLVLAWYEPWYDHASADSSLRHWKPEYKGGQ
                     ADTSLFEFFSSRDPLVWEYDILLAWASGIDGFVVDWFGEKSYENTGIKGLLDTADRLW
                     QRYNGQGFNFQIICSYNERSTGAIDSNMVYIADSLMTHPAYWGTREGRRRPLWIYNQP
                     EVNILPETYRVVADTTLPKDAFLAWNGTEDEIFIPMDVVYPWVQTLPEKWDDLTGKDW
                     GKPYLDQFYARANSPAAQGNLLFGVRSVWPGYDDRSWTLGDKHWMSRRDTLTYRWTWE
                     EAFGYKGALDMPWILIETWNDHNRGSAIQPSLEWNYNFIVKTRDYVRKHKNDCRDYDF
                     ENLGLLVPQHIHQARIAARLRPDQAAAIEAKVERAMDLFFARRHLDAISLADDAAGIA
                     PNKLTVIETPLTGIKICWEPAAHASGYVLYTAKDRSYFEPCSFIRPDSLYLGNVTEYT
                     LQRLDRDKLYLAVVPVNQVINPFANGAWFRNNLTGADVTSTVATAANAEELKPNQLVY
                     ITGTPTHTKEGWEKAVDGITEGWSGTVTALSPEYGNAQKSPWAIFGFADGGLYKFNYL
                     VLQTDNGSEDDSEPGRQTARFKVLVSTASTEEADFKEVYTSRIKSGEETWYALGANVK
                     ARYVKLVLLEPVYSLYRQVVEFQVQTKGRIGAVPANESEEIAEIPQTFKLENNYPNPF
                     NGTTKFRYQLPAQVRVSLRIYDVLGREVAVLVDQEQAPGIYETAWRPNEMASGIYFYR
                     LVAGSYTRIQRMLYLK"
     gene            3665..4198
                     /locus_tag="PKM23_08845"
     CDS             3665..4198
                     /locus_tag="PKM23_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91619.1"
                     /translation="MKKITVLAVLLMGVALMTGCTLSKDFVVTLEKEYTVNYNSTSYS
                     KTEIVDATSFSDDLDKFEDDLKSVNVTKITYTVTYFNGPATQQITSAVLSVAPAEGGS
                     FTTLTTLANVNLMSVAAKEQEVEIDEAGEEVVEDELLGDDHKLQWKFSGTANQAPVNF
                     KIKFKVTCKVKYEKKLI"
     gene            complement(4352..6226)
                     /gene="pepF"
                     /locus_tag="PKM23_08850"
     CDS             complement(4352..6226)
                     /gene="pepF"
                     /locus_tag="PKM23_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNY91620.1"
                     /translation="MKHVEVLFMMLMLTSAGVVQSQTRPRAEVPPEHTWNTADLYPDA
                     ASWQKAKNDLNELTPAITAFQGKVAHSARDLYACLELRSELSRRFGRLSTYAGLLSDQ
                     DTRESEALAMRQEMSQIGVDLASRLAFIEPEIASLTSARIDQFIAQEQGLKVYAFYLR
                     DVLRRKAHKLSAEEEKIVAEAGLMSDAAYSTYSIFSNAELPYPQVKLVDGTSVLVDQA
                     GYGRYRALPNREDRERVFQAFFSTLGQFRRTFGTQLYANVKRDMFYARVRRYENSLSA
                     ALDDNAIPVEVYHALVRSINQNLPTFHRYLRLRQRMLGVPQLAYHDLYAPVVKGVELQ
                     YNNDEARQLILDALAPLGEEYHKVVETAFSSRWIDLYPTTGKRSGAYSNGSAYDVHPY
                     ILMNYSGQYNDVSTLAHELGHALHSHFSNSSQPYPLADYPIFVAEVASTLNEALLIDH
                     ILKTIKDDDVRLSLLMSHLDGIKGTLFRQTQFAEFELAIHEVAERGETLTGDKLTELY
                     RSIFRKYYGADEGICAVDDLYAMEWAYIPHFYYNYYVYQYSTAFTASTALSQKILNHE
                     KGAVESYLAFISAGGSDYPIKLLQKAGVDMTTTAPFSQAMSAMNRTMDEIEKILDKRK
                     "
     gene            6438..>7820
                     /locus_tag="PKM23_08855"
     CDS             6438..>7820
                     /locus_tag="PKM23_08855"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNY91621.1"
                     /translation="MDKKQAKPVSSQVEMLAVERSGRKFIYAPQEKIAIVEVENFPML
                     GKLTALRFIEWVQENPEGIISLPTGKTPEHFIKWVTRYLRDWETPEIRKDLEANGIDA
                     GQKPQMSGLRFVQIDEFYPINPTQVNSFYHYINTFYVRGFGLDPKKGLLINAWSLGVP
                     EGKTPAEVFPDEIVDLSLRTRQPKTRLEKIQQRVITRVDQYCTEYEAQIRAMGGIGFF
                     LGGIGPDGHIGFNVRGSDHFSTTRLTATNYETQAAAASDLGGIEVARNRLVITIGLDT
                     ITYNPRSVAIIIAAGEAKARVIKDAVEQRADNQYPASALHKLPNARFYLTHGAAILLT
                     ERRYENVTKMDPIPEHEVEHIVVDLARERKKHLWELDEEDYASIRSSALVLKRSGKSP
                     MQLGEYIAARLKSKIEEGLAPLEDHTFLHTAPHHDDIILGYWPYIIHLVRSAKNAHHF
                     TYLTSGFNAVT"
BASE COUNT         1710 a         2360 c         2157 g         1593 t
ORIGIN      
        1 aactgccaat gagcggcgtc accgtccgcc tccacgatcc tgccggtgct ctcctcgccc
       61 agcaggtaac cgctgccgac ggcgccttcc tgttcgaccg tctcgctccg ggcagctact
      121 tcctcagttt cgtgccgccg gccggatatg cgctgaccct gttcacggcg gatactacca
      181 gcatggtcaa cagcgatgcc gacatgtcca ccagccgcac catcctgaca gtgctgggcc
      241 agggcgagaa cgatctcacc tggggagccg gtttctatga gctctcatcg cttggcgatt
      301 atgtttggca tgatgtcaat atgaacggcc tgcaagagct gggcgagtca cctgtggaag
      361 atgtcgccgt caccctgctc aacgacgatg gcctgccggt cgactccacc cgcaccgatg
      421 cggacggttt ttactacttc ccgaatctgc cgcctggagg ttatgccctg cgtttcaccc
      481 tccccgcgca ctatatcttc acgggaacgg atcagggcgg caatgacacc cgcgacagcg
      541 acgccgaccg taccaacggc cggaccacgg atatcagcct gggcctggcg gtccatgatt
      601 ccacctggga tgccggcctc atcaagctct cctccgtggg cgactatgtc tgggaagaca
      661 ccgatgccga cggcgtgcag gacggcgacg aactcggcct gcccggcatc cgcatcgtcc
      721 tcaccgatac cctgggccag ccggtcgctg agactgtgac agacgaaaac ggcaactatc
      781 ttttcgatga tgtcgtgccg ggctgctaca ttgtcagagt cgatgaaacg accctgccgc
      841 gcagctacgt caccagcacc tttaacaatc ccatgacggt ctgcctggct ccggatgaac
      901 accgccgtga tgctgatttc ggctaccgct ccaactatgg cagcatcggc gatttcgtct
      961 ggttcgacat caaccgcgac ggcctgcagg atcccggtga acccggcatt cccaatgtcc
     1021 tgctccgcct cgaggagatc aacaccggcg agaccgccaa gcagctcacc aactctgaag
     1081 gcaagtacct cttcagaacc ctgctgcccg gcgaatatcg cgtctatgtc gatgtctcga
     1141 ctctgccaga aggctatttc ctcacctcag gccgcagcga attcattatc tccctcggcg
     1201 atcaggaaaa ctatcgcgag gctgattttg gctactggcg tgaggatgcc ggctgcgaca
     1261 agctcgttct ggcctggtac gaaccgtggt atgaccacgc cagcgccgat tcctccctgc
     1321 gccactggaa gccggaatac aagggcggac aggctgatac cagtctcttc gagttcttct
     1381 catctcgcga tcccctggtt tgggagtatg atatcctcct cgcctgggcc tccggcatcg
     1441 atggtttcgt cgtcgactgg ttcggagaga aatcgtatga aaataccggg atcaaggggc
     1501 ttctggatac agccgaccgg ctgtggcagc gctataacgg ccagggcttc aacttccaga
     1561 tcatctgctc ctacaacgaa cgctcgaccg gcgccatcga ctccaatatg gtctatatcg
     1621 ccgactcgct catgacccat cccgcttatt ggggcacccg cgagggacgc cgccggccgc
     1681 tctggatcta caatcagccg gaggtgaaca tcctgcccga aacctatcgt gtcgtggcgg
     1741 ataccaccct gcccaaggat gccttcctgg cctggaatgg cactgaggac gagatcttca
     1801 tccccatgga cgtggtctat ccctgggtgc agaccctgcc cgaaaagtgg gacgacctca
     1861 ccggcaagga ttggggcaag ccctatctgg accagttcta tgcacgggcc aattctcccg
     1921 ccgcccaggg caatctcctc ttcggcgtgc gcagtgtctg gccgggatat gacgatcgca
     1981 gctggaccct cggcgacaaa cactggatgt cccgccgcga taccctcacc taccgctgga
     2041 cctgggagga ggcctttggc tacaagggcg cactggatat gccgtggatc ctgatcgaaa
     2101 cctggaatga ccacaaccgc ggcagcgcca tccagccctc tctggaatgg aattacaatt
     2161 tcatcgtcaa aacccgcgac tatgtgcgca agcacaagaa cgactgccgc gactatgact
     2221 ttgaaaacct cggcttgctc gtgccgcagc atatccacca ggcgcgcatc gccgcccggc
     2281 tgcgtccgga ccaggctgct gccatcgaag ccaaagtgga aagggccatg gatctcttct
     2341 tcgcccgccg ccacctcgat gccatctccc tggctgatga tgccgccggc atcgcgccga
     2401 acaagctcac tgtcatcgaa accccgctca ctggcatcaa aatctgctgg gagccggccg
     2461 cccacgcctc gggttatgtg ctctataccg ccaaggatcg cagctatttc gagccctgct
     2521 ccttcatccg gcccgattcg ctctacctcg gcaatgtcac cgagtacacc ctgcagcgcc
     2581 tggaccgcga caagctctac ctggctgtcg tgccggtcaa tcaggtcatc aatcccttcg
     2641 ccaacggcgc ctggttccgg aataacctca ccggcgccga tgtgaccagc accgtcgcca
     2701 cggccgccaa tgctgaggag ttgaaaccca accagctcgt ctatatcacc ggcactccga
     2761 ctcacaccaa ggagggctgg gagaaggctg tcgacggcat cacggaagga tggagcggta
     2821 ctgtgacggc cctctcgccc gagtacggta acgcccagaa atccccgtgg gctatcttcg
     2881 gatttgcgga cggtggcctc tacaagttca actaccttgt cctgcaaacc gacaacggca
     2941 gcgaagatga cagcgaaccc ggccgtcaga ccgcccgctt caaggtgctg gtttccaccg
     3001 cgagcaccga agaggccgat ttcaaggagg tttatacctc ccggatcaaa agcggcgaag
     3061 aaacctggta cgccctgggc gccaatgtca aggcccgcta cgtcaagctg gtcctcctcg
     3121 aaccggtcta cagcctctac cggcaggtgg tggagttcca ggtccagacc aagggccgca
     3181 tcggcgctgt gcctgcgaac gagagcgagg agatcgcgga gattccgcag accttcaaac
     3241 tcgaaaacaa ctatcccaat cccttcaatg gaacgaccaa gttccgctat cagctgcccg
     3301 cacaggtccg cgtctccctc aggatttacg acgtcctcgg cagggaagtg gcggtgctgg
     3361 tcgaccagga gcaggcgccc ggcatctatg aaaccgcctg gcgcccgaac gaaatggcga
     3421 gcggcatcta cttctaccgc ctcgtcgccg gaagctacac caggatccag cgcatgctct
     3481 acctcaagta agagagtgca aaagcagcgc tgaatttgca ccataccaat acaagccggg
     3541 atggcagaaa ggccttcccg gctttttttg aggcacaaga agctcaattt cttccgcaca
     3601 atgcggagat tgctgttaca tataatacta aacagcctgg cagttcacta caatgaggaa
     3661 gcctatgaaa aagatcaccg ttttggccgt cctgctgatg ggggtggcgc tgatgaccgg
     3721 ctgcaccctc tccaaggact ttgtcgtcac cctggagaaa gagtacaccg tcaactacaa
     3781 cagcacctcc tactcaaaga cagaaattgt cgatgccacc agctttagcg acgatctgga
     3841 caagttcgag gatgatctca aatcggtcaa cgtgaccaag atcacctaca cggtgaccta
     3901 cttcaacggg ccggccaccc agcagatcac cagtgcagtg ctttcggtcg ctcctgcaga
     3961 gggcggttcc ttcactaccc tgaccacact ggcgaatgtc aatctgatga gtgttgccgc
     4021 gaaggagcag gaggtggaga tcgatgaagc tggtgaggag gttgtggagg atgaactgct
     4081 gggggatgac cacaaactgc agtggaagtt ctccggcacc gcaaaccagg ccccggtcaa
     4141 ctttaaaatc aaattcaagg tgacctgcaa ggtcaaatac gaaaagaagc tgatttgacg
     4201 tcaaaaaagc ccctgaaaag gggctttttt ttaaccttga cctctgacgg cgctgctttt
     4261 atgcgccgcc tttcgtccgc ataccgtaaa cgggcgctgg cgaaacgcgc ttaggcgtga
     4321 atatgccgga gtgcggctgc ttcctgatcg cctatttgcg cttgtcgaga atcttttcga
     4381 tttcatccat ggtccggttc atcgccgaca tcgcctggga aaatggggcg gtagtggtca
     4441 tatccacacc cgccttttga agcagtttga tcggataatc cgatccgccc gcggagatga
     4501 aggccagata ggactccaca gccccttttt catgattgag gatcttttgg gagagggccg
     4561 tcgaggctgt gaaagcggtg gagtactgat agacgtaata attgtaataa aaatgtggga
     4621 tgtaagccca ttccatggcg taaaggtcat ccacagcgca tatcccctca tcagccccgt
     4681 aatatttcct gaatatcgat cggtaaagtt cggtcagttt gtccccggtc agggtttccc
     4741 cccgctccgc gacctcatga atggccagct cgaattcagc gaactgggtc tggcggaaga
     4801 gagttccctt gatgccatca agatgactca tcagcaggga gaggcggaca tcgtcatcct
     4861 tgatagtctt gagaatatga tcgatgagca gggcttcatt cagggtgctg gccacctcgg
     4921 cgacgaagat ggggtaatcg gcaagcggat agggctggct gctgttggaa aagtggctgt
     4981 gcagggcatg gccgagctcg tgcgccaggg tgctgacgtc attgtactgt ccgctgtaat
     5041 tcatcagaat gtaggggtgc acatcgtagg ccgagccgtt ggaataggcg cccgaacgct
     5101 tgccggtggt gggatagagg tcgatccagc gcgagctaaa ggccgtttcg accaccttgt
     5161 gatactcttc gccgagtggg gctaacgcgt ccagaatcag ctgccgcgct tcatcattgt
     5221 tatactggag ctccaccccc ttgacgacgg gggcgtaaag gtcgtggtaa gccagctgcg
     5281 ggacccccaa catgcgctga cgcagccgca ggtagcggtg gaaggtgggc agattttggt
     5341 tgatgcttct gaccagggcg tgatagacct cgaccggtat cgcattgtca tccagggcgg
     5401 cggacaggct gttctcatag cggcgcaccc gcgcatagaa catatcgcgc ttgacgttgg
     5461 catagagctg ggtgccaaag gtgcgacgga actgtcccag ggtagaaaaa aaggcctgaa
     5521 aaaccctctc acgatcttca cggttgggca gggcgcgata gcggccatag cccgcctgat
     5581 cgaccaggac tgaggtgccg tcgaccagtt tcacctgggg ataggggagt tcggcgttgc
     5641 tgaagatgga gtaggtcgaa taggcggcgt cggacatcag gccggcttcg gcaacgatct
     5701 tttcctcctc ggcagagagc ttgtgggcct tgcgccgcag cacatcccgc agataaaagg
     5761 catagacctt gagcccctgc tcctgcgcga tgaactgatc gatccgggcg gaggtgaggg
     5821 aggcgatttc gggttcaatg aaggccaggc ggctggcgag gtcgacgcca atctggctca
     5881 tctcctgtcg cattgccagg gcttcgcttt cacgggtatc ctggtccgag agcagaccgg
     5941 cataggtgga gagacggccg aagcggcgcg atagctcgct gcgcagctcg aggcaggcat
     6001 aaagatcgcg ggcggagtgg gccactttcc cctgaaaagc ggtgatggcc ggggtgagct
     6061 cattgagatc attcttggcc ttttgccagc ttgccgcatc cggatagagg tcagcggtat
     6121 tccaggtgtg ctccggtggc acttcagcgc gcggccgggt ttggctctgc acaaccccgg
     6181 cggatgttaa catcagcatc ataaacagca cctcgacatg tttcatatgg tcggctcctt
     6241 tggaatgatt gcaataatgt acgcattttt atgatcgccg gcaagcctct tgatggcggc
     6301 aggaagtgca tgagatccct cgccggccgg acagcatgcc ctgctgttca agaaatgctt
     6361 ttgaattagt cattttattg atatattact ccggatttgt cattatcact ccaaaccctt
     6421 attggcggaa gagaattatg gacaagaaac aggcgaaacc cgtctcgtcg caagtcgaaa
     6481 tgctggccgt ggaacggagc ggccgtaaat tcatctatgc tcctcaggaa aaaattgcca
     6541 tcgtcgaggt ggaaaatttt cccatgctcg gcaaactcac cgctctccgc ttcatcgaat
     6601 gggtgcagga aaatcctgag ggcatcatct ccctgccgac ggggaaaacg cccgagcact
     6661 ttataaaatg ggtgacccgc tatttgcgcg actgggaaac ccctgagatc cgcaaggatc
     6721 tggaagccaa cggcatcgat gccggccaaa agccgcagat gtccggtctg cgctttgtgc
     6781 agatcgatga gttttacccg atcaatccca cccaggtcaa cagtttttac cattacatca
     6841 atacctttta tgtccgcggt ttcggcctcg atcctaaaaa gggcctgctc attaatgcct
     6901 ggagcctggg tgtccccgag ggtaaaaccc ctgcagaggt ttttccggac gagatcgtcg
     6961 atctctcact gcgtacgcgc cagcccaaaa ccaggctgga aaagatccag cagagggtga
     7021 tcacccgcgt tgaccagtac tgcaccgaat atgaagccca gatccgcgcc atgggcggca
     7081 ttggtttctt tttgggcggc attggaccgg acggccatat cggcttcaac gtccgcggct
     7141 cagaccactt ttccactacc cgtctcacgg ccaccaacta cgagacccag gccgctgctg
     7201 cctcggacct cggcggcatt gaggttgccc gcaaccggct ggtgatcacc atcggtctgg
     7261 ataccatcac ctacaatccc cgctctgttg cgatcatcat cgctgccggc gaggccaagg
     7321 cccgcgtcat caaggatgca gtcgagcaaa gggcggacaa ccaatatcca gcctccgctc
     7381 ttcacaaact gcccaacgcc cggttttatc tcactcacgg cgccgccatc cttctcaccg
     7441 agagacgcta tgaaaatgtc accaaaatgg accccatccc tgaacatgag gtcgaacata
     7501 tcgtcgtgga tctggccagg gagaggaaga aacacctctg ggaactcgac gaggaggatt
     7561 acgcctccat tcgttcctct gccctggtcc tgaagcgaag cggcaaaagc cccatgcagc
     7621 taggggagta tatcgccgcc cggctcaaaa gcaagatcga ggagggcctg gctccgctgg
     7681 aagaccacac cttcctccat accgcccccc atcacgacga catcatcctg ggatactggc
     7741 cctacattat ccacctggtg cgcagtgcca aaaatgccca tcacttcacc tacctcacca
     7801 gcggcttcaa cgcggtcacc
//
LOCUS       DAOPWU010000154         7763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9384_length_7763_cov_4.597561, whole genome shotgun sequence.
ACCESSION   DAOPWU010000154 DAOPWU010000000
VERSION     DAOPWU010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9384_length_7763_cov_4.597561"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PKM23_08860"
     CDS             complement(<1..860)
                     /locus_tag="PKM23_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91622.1"
                     /translation="MNLSARMNRLPGFRTGVLAILLLFALLAATLWPATLDDAFISYR
                     YARHFVEGHGLVFNPGERVEGYSNLLWVLLLSPAIAMGWDAEAASKLIGAGCGLGALL
                     LAMLLLRRHFRASGPVVLLFGAWLATCTGFVYYAVSGLETLFYTLQLLLLIWFLVQKK
                     PGRAALVCTTLLITRPEGMLFILPLTLCVWPGWQEGKAGVMGFLQRLPVKRSGGQGAG
                     KWWLYPLLPAAGLALATLWRWHYYGALLPNTFNAKIKTHWGLLHYAMWHTQTFINYLY
                     QSFAWNEWVL"
     gene            complement(857..2116)
                     /locus_tag="PKM23_08865"
     CDS             complement(857..2116)
                     /locus_tag="PKM23_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91623.1"
                     /translation="MEEWLEGRRGSGWWLGALLVAALYGGTLAFGLTYDDLLLIRNWP
                     LAELMSKWYQPWSERFYRPLINFFFTGEHLLFGGAGWGYHLINLLLTALIIGLYQRIL
                     RAFGVGALTALLCALLWLALPGNGAIITWISAQIDLFALVFMLTAFYLVQSAAAQPHG
                     RAGRIGLSLLMAALAYGSKEISVTLPLLIAAWLLIMRPLPRRQAMGVLLPHLALGAAY
                     FVWRAIVLGARTVGERGFSENPWVAARPLAGIAGMAIRYGEALLSTLYPLFLMPSWLT
                     ALLMLLALGLLAGMFWKRRTLLVPLGRGGLLLLVFTAIGALPNMLDASPRLLGVPTLT
                     VAAMAVLAIGRLLRKAPHLRPQVIILASLLLLSWLHINGEVQACFTQPVYRSTLQYDI
                     NDRRRWPFSDLHNLRYQMRETILRMVQ"
     gene            complement(2135..3169)
                     /locus_tag="PKM23_08870"
     CDS             complement(2135..3169)
                     /locus_tag="PKM23_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91624.1"
                     /translation="MATTTPSRKRIGRLIGLALIFFGVTALRAGDYAADFTRIGVGAR
                     AMAMGGAYVAVANDASASYWNPAGSPGRGALALQVEHMPIFEGLAQYNTAGAHLVFNA
                     QTSVSINWIRLGVDDIPRYDALLGSRYDRYTTGVNRSTGEATGWFSDKEDALFLTFRR
                     SMLLDLAIGGDLAGTVIPTEIAFGVTGKYIHQALDSYSGSGQGLDAGVLVRLMPGWVD
                     EPEPRTWLSLGASVRDLSRTQMSWNTASNHKDEIGRGLQAGLALSHTLPALRLRITAA
                     YDRLFWNEEGDCAGGELTFFNLLSVRGGYYRSELTAGAGLDLAGFSLDYAFVGSDLGN
                     SHRITGAFHL"
     gene            complement(3188..>7763)
                     /locus_tag="PKM23_08875"
     CDS             complement(3188..>7763)
                     /locus_tag="PKM23_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNY91625.1"
                     /translation="GQVRLTLLAEESATRSSGALRLADKPVYSVVDSEGRERAALPLA
                     ELEGWYPARTAELGMDGMFRSQRLVQVYLRPVRYASRQQSLQIVRKLRLRLDIESESG
                     SAAASLARISPASAEPVAVEAQLASTLANYEESKKWRTISGDQAALAKSVQPGGEAQR
                     LRIELGQDGVYAITGREVAGAGVSLSAISPGALSLTHRGRSVAILVEGGSDGTFDEED
                     RIVFIGRHNSAQTFQYSQYSDNAVYWLSWEGSVGARFAESPSSPPQNVADTLASGEFQ
                     LHLERDLIYERTLDNNDPNLDHWYWAPASPDYEFSLPLPVDHLIPGTPLDLTVNMLGL
                     THIYQANPDHHVRLYLNQQQVAEAYWDNQTAVTLQQRIEKPALLPQNNVLRFNLPLDI
                     KGVAIDRILVNYVKLNFTGKLVASEDSLRVLLPASGQRLVRIDGFSTNQIYAFTEEGQ
                     ILTGYRMKQRGSGWSCHLGYQAEAPRNLYFVGRNRLRTVASITLDRPSALRSPANGAD
                     YIIVTHADFAAQAQRLAQHRAAEGLRTAVVDIQDIYDEFNDGIYDPNALRDFISYAYF
                     NWTRPAPLHVLLFGTTTHYMNKRAAEKLNLRSYVPTLMVYTNSWGMTSSDNALVAVSG
                     NDILPDLYIGRFPVSSADQADIMVNKTIDYEQQPVIDEWRRSIGMVYAEGDGGRFIRD
                     ANELVARYTPKRITINRLTTLQGSVHYGNTETLAEMINTGQSLVNFIGHGGGGVYFDN
                     ELFKIEDVKRLSNRNRYPAVFSMTCFVGHFDNPEMPSLGMELVLARDRGAVAHFGSAA
                     KASADGDYYLDIALFNAIFAAQARRMGEIVTLGKLFLIERTNGYWDNLRHFVLLGDPG
                     LNYHISEEEVTIQPSRSAYLVGETIRVSGRASRISTGSAVVTLANEDDSTLVRKVVPL
                     QNGAWECDLLTLTNENISLWRNGEATVRVFASDAREDAAAAATVSVANHAMVQINTDP
                     SAPVHQEPFYFAVTVDETALRNRGGVAGVTLNLSANAVEWQNIALVRQGDNIWRTPEG
                     RKQNEGSRFYYQSVVTAGNGEKIVGAITYATVGFRPDLSIDPTSIRIYGGSPARIDFR
                     IKNGGDKPSGPFHVLVTEGLNASTYKPVGDKLQVSSVPANSDSVLTILWSNPNAGERK
                     FWFQSDADNQIEESNENNNITLAVSRIVTAATGSSGPIYLPESNGYIEIPAGAVAQTA
                     MLNWSQGWDETQVRAAAWSGLLPVKFRFSSGPMLYRYSFADTSVAPVKEIGVVALFDP
                     QDAAARSLVRNSSLRIYGWNARSNTWHGLQSVIDSTQGTVTAALPASLRAFSLLASQD
                     AEAPVITLSVGGQNFADGDVVSRNPLFSAYMEDATGFDLAGAGIRLLLDHQQVDPAGY
                     TLFQNSGSRRSLTLTFAPTLETGSHTMQIEVQDINGNSAMLEAAFHVEGVFSLVSLAN
                     HPNPFPQETTIAFNLSETANRAQLGIYTVSGRLIRSFELQGVTGYMEVDWDGTDDDGN
                     EVANGVYYLKFVAEQGEQRIERIEKMAKLQ"
BASE COUNT         1506 a         2293 c         2614 g         1350 t
ORIGIN      
        1 atcagcaccc actcgttcca ggcgaagctc tggtagagat agttgatgaa ggtctgggtg
       61 tgccacatgg catagtgcaa aagaccccag tgggtcttga tcttggcgtt gaaggtattg
      121 gggagcaggg cgccgtaata gtgccagcgc cacagggtgg cgagggcgag gccggcagcc
      181 gggaggagag gatagagcca ccacttccct gcgccttgcc ctccactgcg cttgacaggc
      241 aggcgctgca gaaagcccat gactcctgcc ttcccctcct gccagcctgg ccacacgcag
      301 agggtcaggg gcaggatgaa aagcatcccc tccggccggg tgatgagcag ggtggtgcac
      361 accagagcgg cgcggcccgg ctttttttgt acaagaaacc agatcagcag gagcagctgc
      421 agggtgtaaa aaagcgtctc cagtcccgag acagcgtaat agacaaatcc ggtgcaggtg
      481 gcgagccagg ccccgaagag caggacgacc ggcccggagg cgcggaaatg gcggcggagc
      541 agcagcatgg ccaggaggag ggcgccgagg ccgcagcccg cgccgatgag cttggaggcc
      601 gcttctgcgt cccatcccat ggcgatggct ggtgaaagca gcaggaccca gagcaggttg
      661 ctatagccct ccaccctttc gccgggattg aagaccaggc cgtgcccctc gacgaaatgg
      721 cgggcgtagc ggtagctgat aaaggcgtcg tcgagcgtgg cgggccagag ggtggccgcc
      781 agcagggcga agaggagaag aatggccagg accccggttc tgaagccggg cagccggttc
      841 attcgggcgc tcaggttcac tgtaccatcc tgaggatagt ttcccgcatc tgatagcgga
      901 gattgtgcaa atcactgaaa ggccagcgcc ggcggtcatt gatatcgtac tggagcgtcg
      961 accggtagac cggctgggtg aaacaggcct gcacctcccc gttgatgtgg agccaggaga
     1021 ggagcagcag ggatgccaaa atgatgacct gcggccgcag gtgcggggcc ttgcgcagca
     1081 gtcgtccgat ggccagcaca gccatggccg cgaccgtgag ggtcggcacg ccgaggaggc
     1141 gcggcgaggc gtcgagcatg ttgggcaggg cgccgatggc ggtgaaaacc aggagcagaa
     1201 ggccgccgcg gcccaaggga acgagcagag tgcgccgctt ccagaacatg cctgcgagaa
     1261 ggccgagcgc gagcagcatc agcagtgcgg taagccaact cggcatcagg aagaggggat
     1321 agagggtcga aagcagggcc tcgccatagc ggatagccat gccggcgatc cccgccaggg
     1381 ggcgggccgc cacccaggga ttctccgaaa agcccctctc ccccaccgtg cgcgcgccga
     1441 gcacgatcgc gcgccagacg aaataggccg ctccgagcgc cagatggggc aggagcactc
     1501 ccatagcctg ccggcgcggc agggggcgca tgatcagaag ccaggcggcg atgagcaggg
     1561 gcagagtaac gctgatctcc ttgctgccat aagccagtgc ggccatgagc agggagagac
     1621 caatccggcc ggcgcggcca tgcggctgtg cggccgccga ctgtacaaga tagaaggcgg
     1681 tgagcataaa aacaagcgca aaaagatcga tttgcgccga tatccaggtg atgatcgccc
     1741 cgttgccggg cagggccagc cagagcaggg cgcataacag ggccgtcaac gccccgaccc
     1801 cgaaggctcg caggatgcgc tgatagagcc cgatgatgag ggcggtgagg aggagattga
     1861 tcagatggta accccagccc gcgccgccga agaggagatg ctccccggtg aaaaagaaat
     1921 tgatcagagg ccggtaaaat cgctccgacc acggctgata ccatttgctc atcagctcgg
     1981 ccagcggcca gttcctgatg agcaaaagat cgtcataggt cagcccgaat gcaagggttc
     2041 cgccgtacaa cgccgcgacc aaaagggcgc ccagccacca cccgcttcct cgtctgcctt
     2101 caagccactc ttccatctca atgcggattc ctgattacag gtgaaaggcc ccggtgatgc
     2161 gatgcgaatt gccaaggtcg ctgccgacaa aggcataatc caggctgaat cctgccaggt
     2221 cgaggccggc cccagcggtc agctcgctgc gataatagcc gccccgtacg ctgagcaggt
     2281 tgaagaaggt gagctctccg ccggcgcaat cgccctcttc attccagaag aggcggtcgt
     2341 aggcggcggt gatgcgcagg cgcagggccg ggagggtatg ggagagggca aggccggcct
     2401 ggaggccgcg gccgatctcg tccttgtggt tggaggcggt attccagctc atctgcgtgc
     2461 gcgagagatc gcggacggag gcccccaggc tcaaccaggt gcgcggctcg ggctcgtcca
     2521 cccagccggg catcaagcgc accagcacgc ccgcatccag cccctggccg ctgccggaat
     2581 agctgtcgag ggcctgatgg atgtatttgc cggtcacccc gaaggcgatc tcggtcggga
     2641 tcacggtgcc ggcgaggtcg ccgccaatgg ccagatcgag gagcatgctg cggcggaagg
     2701 tcagaaagag ggcatcctcc ttgtcggaga accagcccgt ggcctcgccg gtggagcggt
     2761 tgaccccggt ggtatagcgg tcatagcggc tgccgagcag ggcgtcatac cgggggatat
     2821 catcaacccc gagacggatc cagttgatgc tgacgctggt ctgtgcgttg aagacgagat
     2881 gagccccggc ggtgttgtac tgcgccagcc cctcaaagat cggcatgtgc tcgacctgca
     2941 gggccagggc tcctcgcccg ggcgatccgg ccggattcca gtagctggcg ctggcgtcat
     3001 tggcgacggc gacataggcc ccacccatgg ccatggccct tgcccccaca ccgatgcggg
     3061 tgaaatccgc cgcataatcc cccgccctga gggcggtcac accgaagaag atcagtgcca
     3121 gaccaatcag cctgccgatg cgctttctgg aaggagttgt cgttgccatg cgcgaagccc
     3181 tttctcctta ctgcaatttg gccatttttt caatgcgttc gatgcgctgc tcgccctgtt
     3241 cggcgacgaa tttgagatag tagacgccgt tggcaacctc gttgccatcg tcatctgtgc
     3301 cgtcccagtc cacctccatg tagccggtca ctccctgcag ttcgaaagag cggatgagcc
     3361 gccccgaaac cgtatagatg cccagctgcg cgcggttggc ggtctcggag agattgaagg
     3421 cgatggtggt ctcctgaggg aagggattgg gatggttggc caggctgacc aggctgaaca
     3481 ccccctcgac gtggaaggcc gcctccagca tggcactatt gccgttgatg tcctgcacct
     3541 cgatctgcat ggtgtggctg ccggtctcaa gggtcggggc gaaggtcagg gtgagggagc
     3601 gccgcgagcc ggagttctga aaaagggtat acccggccgg atcgacctgc tgatgatcga
     3661 gcagaaggcg gatgcctgcg ccggcgagat caaaaccggt cgcatcctcc atgtaggccg
     3721 agaagagggg attgcgcgag accacatccc cgtcggcaaa gttctgcccg ccgacgctga
     3781 gggtgatcac cggggcctcc gcatcctggc tggccaggag gctgaaggcg cgcagggagg
     3841 caggcagcgc ggctgtcact gtcccctgcg tggaatcgat caccgactgg aggccgtgcc
     3901 aggtgttcga cctggcattc cagccgtaga tgcgcaggct gctgttgcgg accagcgacc
     3961 gggcggcggc atcctgggga tcgaagaggg caaccacccc gatctccttg accggggcca
     4021 cggaggtatc ggcaaagctg tagcggtaga gcatcgggcc cgaagagaag cggaatttaa
     4081 ccggcagcag accggaccag gctgccgctc ggacctgggt ttcgtcccac ccctggctcc
     4141 agttcagcat cgccgtctgg gccacagccc cggccgggat ttcgatataa ccgttgctct
     4201 cgggcagata gatgggtccg ctgctgccgg tggcggccgt gacgatgcgt gataccgcca
     4261 gggtgatgtt gttgttttcg ttgctctcct cgatctggtt gtcggcatcg gactggaacc
     4321 agaatttgcg ctctcccgca ttgggattgc tccagaggat ggtgagcacc gaatcgctgt
     4381 tggcgggaac cgagctcacc tgcagcttgt cccccaccgg cttgtaggta gaggcgttca
     4441 atccttcggt caccagaacg tggaaggggc ccgagggttt gtcgccgccg tttttgatgc
     4501 ggaaatcgat gcgcgccggg ctccctccgt agatgcggat cgaagtcgga tcgatcgaga
     4561 ggtcgggccg gaagccgacg gtggcgtagg tgatggcgcc aacaatcttt tcgccgttgc
     4621 ccgcagtcac cacgctctgg taataaaagc gcgagccctc gttctgtttg cggccctcgg
     4681 gcgtgcgcca gatattatcg ccctggcgca ccagggcgat gttttgccac tcgactgcat
     4741 tggcggagag attgagggtc acacccgcca ccccgccgcg attgcgcagg gcggtttcat
     4801 cgaccgtcac cgcaaaataa aagggctcct ggtgaaccgg cgcggaggga tcggtattga
     4861 tctgcaccat ggcatggttc gccacagata cggtcgccgc tgctgccgca tcctcccgcg
     4921 catcggaagc gaagacccgg acagtcgcct cgccgttcct ccacagggag atattttcat
     4981 tggtcagggt gaggaggtcg cactcccagg cgccgttctg caggggcacc actttgcgca
     5041 ccagggttga gtcgtcctcg ttggcgaggg tcaccacggc gctcccggtg ctgattctgc
     5101 tcgcccggcc gctgacgcgg atggtctcgc cgaccaggta ggcgctgcgc gagggttgaa
     5161 tggtgacctc ctcctccgaa atatggtagt tcagaccggg atcaccgagc aggacgaaat
     5221 ggcggagatt gtcccagtag ccgttggtcc tttcgatcag gaagagcttg ccgagggtga
     5281 caatctcgcc catgcgccgc gcctgagcgg caaaaatggc attgaagagg gcgatgtcga
     5341 ggtaataatc cccgtcggcc gaggccttgg ccgcggaacc gaagtgggcc actgcgccgc
     5401 ggtcgcgggc gagcaccagt tccatcccca gcgagggcat ctcgggattg tcgaaatggc
     5461 cgacgaaaca ggtcatgctg aagacggcgg gatagcgatt ccggttgctg aggcgcttga
     5521 cgtcctcgat cttgaaaagt tcgttgtcga aatagacccc gccgccgccg tggccgatga
     5581 aattgaccag gctctgaccc gtattgatca tctcggcaag ggtctcggtg ttgccgtaat
     5641 ggaccgatcc ctgcagggtg gtcagccggt ttatggtgat ccgtttcggt gtatagcggg
     5701 cgaccagctc gttggcatca cgaatgaagc gaccgccgtc gccttcggca tagaccatgc
     5761 cgatgctgcg gcgccactcg tcgatcaccg gctgctgctc ataatcgatg gtcttgttga
     5821 ccataatgtc ggcctgatcc gcgctgctga cgggaaagcg gccgatgtag aggtcgggca
     5881 ggatgtcatt gccgctgacg gcgaccaggg cattgtccga tgaggtcatg ccccaggagt
     5941 tggtatagac catcagggtc gggacgtagc ttctgagatt gagtttctcg gcggcccgct
     6001 tgttcatata gtgggtagtg gtgccgaaga gcagcacatg caagggggca ggacgagtcc
     6061 agttgaaata ggcataggaa ataaagtcgc gcagggcatt gggatcatag atgccgtcgt
     6121 tgaattcgtc gtagatatcc tggatgtcga ccacggccgt acgcagcccc tccgcggcgc
     6181 ggtgttgggc cagacgctgc gcctgagcgg cgaaatcggc atgggtgacg atgatataat
     6241 cggcgccgtt tgcgggcgaa cgcagggcgg aaggccggtc gagggtgatg gaggccaccg
     6301 tccgaagccg gttgcggccg acaaaataga ggttgcgcgg ggcctcggcc tggtagccga
     6361 gatggcagct ccagccgctc ccgcgctgct tcatccggta cccggtcagg atctggccct
     6421 cctcggtgaa ggcgtagatc tgattggtgc tgaagccatc gatacgcacc aggcgctgtc
     6481 cgctcgcggg cagaagcacg cgcagagagt cctcgctggc gaccagcttg ccggtgaaat
     6541 tgagcttgac atagttgacc agaatccggt cgattgcaac ccccttgatg tccagcggca
     6601 gattgaagcg cagaacattg ttctggggga ggagggccgg cttctcgatt ctctgctgga
     6661 gggtgaccgc ggtctggttg tcccagtagg cctccgcgac ctgctgctgg ttgagataga
     6721 ggcgcacatg atggtcggga ttggcctgat agatgtgggt caatccgagc atgttcacgg
     6781 tcagatccag gggcgtgccg gggataagat ggtcgaccgg cagcggcagg ctgaattcat
     6841 aatccggaga agcgggcgcc cagtaccaat ggtcgagatt ggggtcgttg ttgtccaggg
     6901 tgcgctcgta gatcagatcg cgctcgagat ggagctgaaa ctcgccgctt gccagggtgt
     6961 cggcaacatt ctggggcgga gaggagggcg actcggcaaa acgggcgccc acgctgccct
     7021 cccaggagag ccagtaaacg gcgttgtcgg aatactggct gtattgaaag gtctgtgcgc
     7081 tgttatggcg gccgatgaag acaatccggt cctcctcatc gaaggtgccg tcgctccccc
     7141 cctcgaccag aatggcgaca cttcgaccgc gatgagtcag ggagagggcg ccgggtgaga
     7201 tggcgctcag ggagacccct gcgccggcca cctcgcggcc ggtgatggca tagaccccgt
     7261 cctgccccag ttcgatgcgc agacgctgcg cctcgccccc gggctgcacg gatttggcca
     7321 gggcggcctg gtcaccgctg atggtgcgcc actttttgct ctcctcatag ttggccaggg
     7381 tcgaggccag ctgagcctca accgccaccg gctcggcgga ggcggggctg atccgcgcca
     7441 gcgaggccgc agcagagccg ctctcgctct caatgtcgag acgcaggcgg agtttacgca
     7501 caatctgcag cgactgttgg cggctggcgt agcgcacagg acgaagatag acctgcacca
     7561 gccgctggct gcggaacatc ccgtccatac ccagctcggc agtccgggcc ggataccacc
     7621 cctccagttc cgccagcggc agcgcggctc gctcccgccc ttcggaatcg acgacgctgt
     7681 agaccggctt gtccgcaagc cgaagggcac cagagctgcg cgttgcgctc tcctcggcaa
     7741 gaagggtaag gcggacctgc ccc
//
LOCUS       DAOPWU010000155         7745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9419_length_7745_cov_4.852926, whole genome shotgun sequence.
ACCESSION   DAOPWU010000155 DAOPWU010000000
VERSION     DAOPWU010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9419_length_7745_cov_4.852926"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKM23_08880"
     CDS             complement(<1..367)
                     /locus_tag="PKM23_08880"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875978.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNY91626.1"
                     /translation="MQPTKILLDESEMPRQYYNIAADFPTPAAPPLGPDGKPITPEML
                     APVFPMNLIELEMSTQRWIDIPEEVLGIYKMWRPSPLVRAFGLEKVLGTPARIYYKNE
                     SLSPAGSHKPNAAVPQAYYN"
     gene            complement(612..1613)
                     /gene="pdxA"
                     /locus_tag="PKM23_08885"
     CDS             complement(612..1613)
                     /gene="pdxA"
                     /locus_tag="PKM23_08885"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNY91627.1"
                     /translation="MKPLIGITLGDPAGIGPEIVLKALAERRVQETLVPLLFGDRVVI
                     EEAMAQTGLVRPLRRLHKPAENGAAGDELPYFDTGSLEGPVEMGRVDGSCGRAAFAAI
                     RTAIEWARAGWIDALATAPINKASLQQGGVPFIDHSAMLAALTESPKAMTLFVVGRLR
                     IFFLTKHIPLSRVSAALTIPGVVEGLVESDRGLQQLRLPRRRIALAALNPHASDGGLF
                     GDEEMRILAPAVEEARGLGLEVCGPVPADSVFHQCSQGEYDAVLSLYHDQGHIAAKTL
                     DFHRTVSFTLGLPFLRTTPDHGTAFAIAGTNQANPLSMIEALLAAAEYCRRRGQNIS"
     gene            complement(1610..2539)
                     /locus_tag="PKM23_08890"
     CDS             complement(1610..2539)
                     /locus_tag="PKM23_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNY91628.1"
                     /translation="MIHRNSGLSTSWQSGLIRDGGELLDVLGASGLSGLEIDYRVSGE
                     ALRQMEPRLRSGEFTVLSLHNYCPTPPGYEGHPNVAALFNPASLDTEERKLAVKYSIR
                     TIQTAADLGARLVVFHLGVIDMDFRMKELFQLHEEEGEDSPLFQERIQELQRERAEKA
                     GAHLTPLLRTVEAVHTEAHKRSILMGVENRYRLSQIPVGDEFDLLFNEFEGGELRYWH
                     DVGHAEVFARLGLLDHERDLLRRFQKRLAGMHIHDIAGFKDHLAPGMGTFDFSLLPPY
                     LGEESLRIIEAHDPVDLAQLREGVRLLQEKGVL"
     gene            complement(2544..5042)
                     /locus_tag="PKM23_08895"
     CDS             complement(2544..5042)
                     /locus_tag="PKM23_08895"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91629.1"
                     /translation="MNDQNPLRTGPAQDAAAEGDLDKEQMMSKGFKPVPVELLRWQCD
                     QYVQSFQSTEELESYRKILGQERALEALTLGLEMDYPGYNIFVSGDTGTGRRTTVNFM
                     LHDERFRKPVPADICYVHNFQNPDMPRVLQLAAGGGVRFKKMMEDVIAQLRVAVVQVL
                     ESERTRKRREAVLEKYAHQQAEKLQQFEKRAQKDQFTLVQVQMGPYTKPDVLPVIEGQ
                     PVPLENLGELVAQGKIDDKRVAEIQQAYIELNKELAKLFQENQRLQREKKAKVAELEY
                     TIVKPVIEEAFNDVRAAFADKVVSDYLDAVLKSLLENLHKFNARPEGEEGERKEGEVR
                     SRDQFLEYRVNVLVNNADKEYAPVIHENSPTQSRLFGSIERLMDGNGQWHTDFTHIRA
                     GSLLQANGGYLVLHARDVLLEPGVWPMLKRMLKNAETEINANDPVLLLAMTAMKPEPI
                     PLSVKVIMLGDPFLYQLLYSADDDFRKIFKIKAEFDSVMPNDATNLRRYAEFIKRMCD
                     EHELLPYDASAVAEVAEYGVRLAGRQNKLSTRFTDILDLLREANYYARADKTDIVAKK
                     HVERAVTAAKHRLNLIEEKIRESILEGTLLIDTDGARIGQVNGLTVLSMGDYHFGQPA
                     RITAQCSMGREGIINIEREVAMSGPTHDKGVLIISGYLQGKFSQDKPLSINASICFEQ
                     SYGGIDGDSASSTEVYALLSRLSGQPVRQDLAVTGSVNQNGEIQPIGGVNEKIEGFFE
                     VCRMRGLTGSQGVLIPRLNVPDLMLKNSVIDAVQAGQFVIYAIDRIDEGIEILTGIPA
                     GERDNQGCYPPDTIFGQVDARLSAYAEGLRRFRE"
     gene            complement(5035..6837)
                     /gene="dnaG"
                     /locus_tag="PKM23_08900"
     CDS             complement(5035..6837)
                     /gene="dnaG"
                     /locus_tag="PKM23_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNY91630.1"
                     /translation="MRRLAIPQDKIEQVRLASDVVDVVSGYVTLKKRGKNYFGLCPFH
                     HEKSASFSVNPDLQIFHCFGCGAGGNVFTFIMRIEGLTFPESVRMLAKAAGIVLPDEK
                     EDPGQLQEKEALYYANGLANEFFMANLLREAEAEPARRYLASRGLTPEAFARFHIGYS
                     LNKWDGLLQHARAKSLNPDLLVKAGLAIRKEDGKIYDRFRGRITFAIHSLSKQVVAFG
                     ARRIVQDDSPKYINSPETGIYQKRYILYGLHLAREAVRQADRVVIVEGYTDLISLHMA
                     GIEQVVATSGTAMTEEHARLLRRYTAHASILYDGDSAGAAASLRGAEILLANGFDVRI
                     AMLPGGQDPDEFVRQSGADAVRRHLQEGMALIDFKMKSLEQKGLLRTPSQKAESTREL
                     LASIARVADTIQKSFLLRDLAEKLNVEEAVLWAEVHKLERRLRQQPRSEEKKSQPVKG
                     DRYLESKRGAAELGLLEMALTQPELLPRILSLITADEFIHVDVRAVFEGLELDALEGV
                     PFEIKRYLAMIRDPGVAGVISRALEQLHKPPHPARYTFDCLNTIRIAKLDERIEALRA
                     RMKGGEKPGEELLAEYRAVVEERKRLEQIEFAFE"
     gene            6994..>7745
                     /locus_tag="PKM23_08905"
     CDS             6994..>7745
                     /locus_tag="PKM23_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNY91631.1"
                     /translation="MAKRTLPYLLEESAAKYPGNILLWERFGDRYEGTTYSEAKQQVH
                     LFAAGLLSLGIQKGDRIALISEGRNAWVIAELGILYTGAINVPISVKVDELADLKFRI
                     SHSGCRMAVVSGSQAPKIIAIKNDLPMLENMVVLDDSGRRGPDEITLQNLLQKGKEYL
                     EHHAGLFEETWQGVEENDPANICYTSGTTADPKGIILTHRNYTANVEQACSLLPIPEW
                     YLSLLILPWDHSFAHTAGIYTLMKNGASMASVP"
BASE COUNT         1422 a         2396 c         2405 g         1522 t
ORIGIN      
        1 tgttgtagta ggcctggggt acggcggcgt tgggcttgtg gctgccggca gggctgaggc
       61 tctcgttctt gtaatagatg cgcgccggcg tgcccagaac cttctccagc ccgaatgccc
      121 gcaccagggg cgatggccgc cacattttgt agatccccag cacctcttcg ggaatgtcga
      181 tccagcgctg cgtgctcatc tccagttcga tcaggttcat cgggaaaacc ggggcgagca
      241 tctccggcgt gattggcttg ccgtccggac cgagcggcgg agcggcaggt gtgggaaaat
      301 cggcggcgat gttgtagtac tggcgcggca tttcgctttc gtcgagcagg atcttggtgg
      361 gttgcatggc tttcctccat ggggttgata gtaaaaaaaa agcccgctgc tttcgtatac
      421 gcagcgggct gaaattagcc gatcgatggt gagggttgct atccgatgca cctctctatc
      481 ccgctccgta tgaagatacg ccaccaccac cagtttttgc gggtcgtttt gcgttgaacg
      541 gatgtgggat gattgttata catcgtttcc tcagtaaggt tgacgcaata tagccattta
      601 ttcagaacct gtcaagaaat attttgcccg cgccggcggc aatattccgc cgccgcaagc
      661 agggcctcga tcatgctcag gggattggcc tggttcgtgc cggcgatggc gaaggcggtg
      721 ccgtggtccg gggtggtgcg cagaaagggc agcccgaggg tgaaggagac cgtgcgatga
      781 aaatcaagag tcttggcggc gatgtggccc tggtcgtgat agagggagag gacggcatcg
      841 tactcgccct ggctgcactg atgaaagacc gagtcggctg gaaccggtcc gcagacctcc
      901 agaccgagtc cgcgcgcctc ctcgacggcg ggggcaagaa tgcgcatctc ttcatccccg
      961 aagagcccgc catcactggc gtgcggattg agtgcggcca gggcgatccg ccgccgcggc
     1021 agcctgagct gctgcagacc acggtcgctc tcaaccagcc cctcgaccac ccctgggatg
     1081 gtcagcgcag cggacacccg gctcaggggg atatgcttgg tgagaaagaa gatgcgcagc
     1141 cggccgacca caaagagggt catcgccttc gggctctccg tgagggcggc gagcatggcg
     1201 ctgtgatcga taaaggggac gcccccctgc tgcagcgagg ccttgttgat cggggcggtc
     1261 gcgagggcgt cgatccagcc cgcccgcgcc cattcgatcg cggtccggat ggcggcgaac
     1321 gccgcccggc cgcagctgcc gtcgacccgt cccatctcca ccggtccttc aagcgagcca
     1381 gtgtcaaaat agggcagctc gtcccctgcc gcgccattct ccgcaggctt gtgcaggcgg
     1441 cgcaggggcc tgaccagacc ggtctgtgcc atggcctctt cgatgacaac ccgatcccca
     1501 aagaggagag gaacgagcgt ctcctgcacc cgcctctccg ccagagcctt gagcacgatc
     1561 tccgggccga tgccggccgg atcgcccagg gtgatgccga tcaggggctt cacagcaccc
     1621 ccttttcctg gagcaagcga accccctcgc gcagctgcgc cagatcaacc ggatcatgcg
     1681 cctcgatgat gcgcaggctc tcctccccca gatagggcgg cagcaggctg aaatcaaagg
     1741 tgcccatgcc gggggcgaga tggtccttga agccggcgat atcgtggata tgcatgccgg
     1801 cgaggcgttt ttggaagcgc cgcagcaggt cgcgctcatg gtcgagcagg cccagccggg
     1861 cgaagacctc ggcgtggccg acgtcgtgcc agtagcgcag ttcccctccc tcgaattcgt
     1921 tgaagaggag gtcaaactcg tcgccgaccg gaatctgcga caggcggtag cgattctcca
     1981 cccccatgag aatgctgcgc ttgtgcgcct cggtatgcac cgcctcgacg gtccgcagca
     2041 ggggggtcag atgggcgccc gccttctccg cccgctcccg ctgcagctcc tgtatcctct
     2101 cctgaaagag gggggagtct tctccctcct cttcatggag ttggaaaagt tccttcatcc
     2161 ggaaatccat atcgatgaca ccgagatgaa agacgaccag gcgcgccccg agatcggcgg
     2221 cggtctggat ggtccggatg ctgtacttga cagcgagctt gcgctcctcc gtgtcgaggg
     2281 aagcgggatt gaaaagcgct gcgacgttgg gatgcccttc gtagccgggc ggggtcggac
     2341 agtaattgtg caggctgagc accgtgaatt cgccgctgcg cagccgcggc tccatctgcc
     2401 gcagggcttc cccgctgacc cggtagtcga tctcgaggcc ggaaaggccg ctggcgccga
     2461 ggacgtcgag cagttcgccg ccatcgcgga tcaggcccga ctgccaggag gtggataaac
     2521 cgctgtttct gtggatcatc gtttcactcc ctgaaccgcc gcagcccctc ggcgtaggcg
     2581 ctgaggcgcg catcgacctg gccgaagatg gtatcggggg gatagcaccc ctggttgtcg
     2641 cgctcgccgg ccgggatgcc cgtgaggatc tcgatgccct cgtcgatgcg gtcgatggcg
     2701 taaatgacaa actgcccggc ctgcaccgca tcgatgacgg aatttttcag catcaggtcc
     2761 ggcacattga ggcggggaat gagcaccccc tggctgccgg tgaggccgcg catgcggcaa
     2821 acctcgaaaa agccctcgat cttttcgttg accccgccga tgggctggat ctcgccattc
     2881 tggttgaccg agcccgtcac cgccaggtcc tgccggacgg gctggccgga gaggcgcgag
     2941 agcagggcgt agacctcggt ggaggaggcg ctgtcgccgt caatgccgcc ataggactgc
     3001 tcgaaacaga tggaggcgtt gatcgagagg ggcttgtcct ggctgaattt gccctgcaga
     3061 tagccggaga tgatcagcac ccccttgtcg tgggtcggac cgctcatggc gacctcgcgc
     3121 tcgatgttga tgattccctc ccgccccatc gagcactggg cggtgatccg ggccggctgg
     3181 ccgaaatgat agtcgcccat gctcaggacg gtgaggccgt tgacctggcc gatacgggcg
     3241 ccgtcggtat cgatcagcag cgtcccctcc aggatgctct cgcggatctt ctcctcgatc
     3301 agattgaggc ggtgtttagc cgcggtcacg gcgcgctcga cgtgcttctt ggcgacgatg
     3361 tcggtcttgt ccgcacgggc gtaataattg gcctcgcgca gcagatcgag gatgtcggtg
     3421 aaacgggtgc tgagtttgtt ctggcggccg gccagacgca ccccgtattc cgccacctca
     3481 gccaccgcgc tcgcatcgta gggcagcagc tcgtgctcgt cgcacatccg cttgatgaac
     3541 tcggcgtagc ggcgcaggtt ggtggcatca ttgggcatca ccgaatcgaa ttcggccttg
     3601 atcttgaaaa tcttgcggaa atcgtcgtcc gccgaataaa gcagctggta gagaaaggga
     3661 tccccgagca tgatgacctt gacgctgagg ggaatgggct cgggcttcat cgcggtcatg
     3721 gccagcagca ggaccggatc attggcattg atctcggtct cggcgttctt gagcatgcgc
     3781 ttgagcatag gccagacccc gggctcgagc agcacatccc gggcgtgcag gaccagataa
     3841 ccgccgttgg cctgcaggag tgaaccggcg cggatatggg tgaaatcggt gtgccactgg
     3901 ccgttgccat ccatgagccg ctcgatgctg ccgaaaagcc ggctctgcgt aggtgaattt
     3961 tcatgaatga ccggggcgta ctccttgtca gcgttgttga ccagaacgtt gacgcggtac
     4021 tcgaggaact ggtcgcgcga cctgacctcg ccctcctttc gctccccctc ctccccctcc
     4081 ggccgggcat tgaatttgtg cagattttcg agcaggctct tgagcacggc atcgagataa
     4141 tcgctcacca ctttgtcggc aaaggcggcg cgcacatcgt taaaggcctc ctcgatcacc
     4201 ggcttgacga tggtatactc cagctcggcc accttggcct ttttctcgcg ctgcaggcgc
     4261 tggttctcct ggaagagctt ggcgagctcc ttgttgagct cgatgtaggc ctgctgaatc
     4321 tccgcaaccc gcttgtcgtc gatcttgccc tgcgcgacca gctcgcccag attctcgagg
     4381 ggcacgggct ggccctcgat gaccggcagc acatcgggct tggtgtaggg ccccatctgc
     4441 acctgcacga gggtgaactg gtctttctgc gcccgctttt cgaactgctg aagcttttcg
     4501 gcctgttgat gggcatactt ttccagcacg gcctcgcgcc gcttgcgcgt ccgttcgctc
     4561 tccagcacct gcacaaccgc aacccggagc tgggcgatga catcttccat cattttttta
     4621 aaacggactc ctccgcccgc cgccagctgc agcacccggg gcatgtccgg attttgaaaa
     4681 ttatgcacat aacagatgtc ggcggggacc ggcttgcgaa agcgctcgtc gtggagcata
     4741 aaattcaccg tcgtccgccg tccggtaccg gtatcgccgg agacgaagat gttgtagccc
     4801 ggataatcca tctccaggcc gagggtgagg gcctcgaggg cgcgctcctg cccgagaatc
     4861 ttgcgatagc tctccagctc ctcggtcgac tgaaaggact gtacatactg gtcacactgc
     4921 cagcgcagca gttccaccgg aaccggtttg aatcccttgc tcatcatctg ctccttgtca
     4981 agatcccctt cggccgcggc atcctgcgcc ggccccgtcc gcaaggggtt ctggtcattc
     5041 aaaagcgaat tcgatctgtt cgaggcgctt gcgctcctcc accacggcgc ggtactccgc
     5101 gagcagctcc tcgccgggct tttcgcctcc tttcatgcgc gcacgcaagg cctcgatgcg
     5161 ctcatccagc ttggcgatgc ggatggtgtt gaggcagtcg aaggtataac gggcgggatg
     5221 ggggggtttg tgcagctgct ccagggcgcg agaaatgact ccggctaccc ccggatcgcg
     5281 gatcatggcg agatagcgtt tgatttcaaa ggggacgccc tcgagggcgt cgagctcaag
     5341 tccctcaaag acggcgcgta catcaacatg gatgaattca tcggctgtga tcagggagag
     5401 gatccgcggc agcagctccg gctgggtcag cgccatctcg aggaggccga gctccgccgc
     5461 cccccgcttg ctctcgagat agcggtcccc cttgaccggc tggctcttct tctcttcgga
     5521 ccgcggctgc tgccgcagcc gccgctcgag cttgtggacc tccgcccaga gcaccgcctc
     5581 ctcgacattg agcttctcgg ccagatcgcg caggagaaaa gacttctgga tggtatcggc
     5641 gacccgggcg atcgaggcga gcagctcgcg ggtcgactcc gctttctggc tgggggtgcg
     5701 cagcagacct ttctgctcca ggctcttcat cttgaaatcg atcagggcca tcccctcctg
     5761 gagatggcgc cggaccgcat cggccccgct ctggcgcacg aattcatcgg ggtcctgacc
     5821 gcccggcagc atggcgatgc ggacgtcaaa gccgttggca agaaggatct ccgcgccacg
     5881 cagcgaagcc gctgcaccgg ccgaatcccc gtcatacagg atggaggcgt gggcggtgta
     5941 gcgccgcagc aggcgggcat gctcttcggt catggccgtg cccgaggtgg ccaccacctg
     6001 ctcgatgccg gccatatgca ggctgatcag atcagtgtag ccctcgacaa tcacgacacg
     6061 gtcggcctgg cgtaccgcct cgcgggcgag gtggaggcca tagaggatgt agcgcttttg
     6121 atagatcccg gtctcgggcg agttgatgta cttgggcgag tcgtcctgaa cgatccggcg
     6181 cgctccgaag gcgaccacct gcttgctcag ggagtggatg gcgaaagtga tgcgcccgcg
     6241 gaaacggtcg tagatcttgc catcctcttt cctgatcgcc agtccggcct tgaccagcag
     6301 atcgggattg agcgatttgg cgcgggcgtg ctggagcagt ccgtcccatt tgttcaggct
     6361 gtagccgata tggaagcggg caaaggcttc cggggtcagc ccgcgtgagg cgaggtagcg
     6421 gcgcgccggt tcggcttcgg cctcgcgcag cagattggcc ataaaaaact cattggccag
     6481 gccgttggca tagtaaaggg cctccttctc ctggagctgg cctggatcct ccttctcatc
     6541 gggcaggaca atgcccgcgg ccttggcgag catgcgcacg ctctcgggga aggtgagccc
     6601 ctcgatgcgc atgatgaaag tgaagacgtt cccgccggcg ccgcagccga agcagtgaaa
     6661 aatctgcaga tcgggattga cgctgaagga ggcgctcttc tcgtggtgaa aggggcagag
     6721 gccgaaatag ttcttgccgc gctttttaag ggtgacatag ccggagacca catcgacgac
     6781 atcactcgcc aaccgcacct gctctatttt atcctgggga atcgccaacc gacgcatcca
     6841 tccctgttca ttcaggagaa ttagcttgac tttttagcca attgtggtta tgatgatatc
     6901 atgccgtttc gcgcaaccaa ctaatcccgg ggtagcggcg gggcacccct ccgggagccc
     6961 ctttcaccag ctaattaaaa ggagtcaacc atcgtggcta aaaggaccct gccctatctc
     7021 ctcgaagaga gtgcggcaaa atatcccggc aacatcctgc tctgggaaag attcggcgac
     7081 cggtatgagg ggaccaccta cagcgaagca aagcagcagg tgcatctctt cgccgccggt
     7141 ctgctcagcc ttggcatcca gaagggcgac cgcatcgccc taatctccga aggccgcaat
     7201 gcgtgggtga tcgccgagct cggcattctc tacaccggcg ccatcaatgt gccgatctcg
     7261 gtcaaggtgg atgagcttgc ggatctcaag ttccgcatca gccactccgg ctgccgcatg
     7321 gccgtggtct cgggcagcca ggcccccaag atcatcgcga ttaaaaacga tctgcccatg
     7381 ctcgaaaaca tggtcgtctt ggacgacagc ggcaggcgcg gtccggatga aattacgtta
     7441 caaaatctgc tgcaaaaagg caaggaatat ctggaacacc acgccgggct ctttgaggag
     7501 acctggcagg gggtcgagga gaacgatccc gccaatatct gctatacctc cggcaccacc
     7561 gccgacccca agggcatcat cctcacccat cgcaactata ccgccaatgt tgaacaagcc
     7621 tgctctctgc tgccgattcc ggaatggtac ctctccctgc tcatcctgcc ctgggaccac
     7681 agtttcgccc acaccgccgg catctacacc ctgatgaaaa acggcgccag catggcctcc
     7741 gtccc
//
LOCUS       DAOPWU010000156         7672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9540_length_7672_cov_4.780228, whole genome shotgun sequence.
ACCESSION   DAOPWU010000156 DAOPWU010000000
VERSION     DAOPWU010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9540_length_7672_cov_4.780228"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKM23_08910"
     CDS             <1..462
                     /locus_tag="PKM23_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNY91632.1"
                     /translation="RGSADLITTDGSISIGSVEGAVAARTSGGGISLGSATGRAQLAT
                     SGGSISVDAVKGAIEASTSGGGITAAITAQPQQPCRLATSGGSIKVHLAPDLRLDINA
                     STSGGHVHSEFDLLIHGGMSRSSLQGKLNGGGPELYLRTSGGNITLAKPEL"
     gene            753..1214
                     /locus_tag="PKM23_08915"
     CDS             753..1214
                     /locus_tag="PKM23_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91633.1"
                     /translation="MKKALLLIALIALAGCSKQMIRFDQYSVAMNLTVDADSSVYLGD
                     GDKFNGVLFLAPILREENQPVSTVKVIQNYGRYYLCADEFRNLWMIEPTSDGTEGKIK
                     AIDVTPEDETDQLRNISLSRYGTEEKACIRFRFNGKEIFINQKGGLNEECK"
     gene            1198..2313
                     /locus_tag="PKM23_08920"
     CDS             1198..2313
                     /locus_tag="PKM23_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91634.1"
                     /translation="MKSANSWMMLFLLLLAPLLQAQDVQDLYEQAKEALRQADYQTCL
                     DKIAAAKAEISQDPQLDPNGVFMKRLLPEVERNGQNMAAIMLALEELYDSSQSGVTFA
                     DLGLNQEAITQYHAQARDASDDLQRRRDEILASRELAPEYRTALLRAPVLTRIEKLAS
                     TGIMEKLTGKFSQMVGLLTDSLSAVDNRFRMAEARLAKLQKSAAASKQEIEKIKGEMA
                     KLNTERLSYINTISEMLAGEASPEMEPIRVALSENQVDDVFRGVIESEIRRVKGITTI
                     DSVGYKELLKDYDRIRNYNRIFAKNNIAPDQAALLASYDAAIKAVKVEVPKTTNWTLI
                     YIIIGVLLLLLLWALYKLFGGSKKKSPPPPEYKSYQP"
     gene            2567..3001
                     /locus_tag="PKM23_08925"
     CDS             2567..3001
                     /locus_tag="PKM23_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91635.1"
                     /translation="MLSKKKMEIGIEVEALCNKCKAATVHVVVAIKSQDMVRVMCKTC
                     QSLHRFKAPESKGVKKAAKVQKAQKVKLTPEARESKKWNKLLLAADPEKAVAYSMSSV
                     YAENDIIQHTKFGLGVVVKILDPSKISVVFEDGVKNMVQNLK"
     gene            complement(3070..4743)
                     /locus_tag="PKM23_08930"
     CDS             complement(3070..4743)
                     /locus_tag="PKM23_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNY91636.1"
                     /translation="MVALQKRTLCELFRAGVAEFSRDIVFSFVGEEPYTYRQVEQQVH
                     QLVQSLRQRGIRPGDRIALLGDNSPSWCIAYLAIVSMGAVVVPILTEFHKSEIHHIIR
                     NSNARGLFVTSKLVHKVVELNIRDLVFVASLDNQELPLDEKKWVRLSALLAGEAPARK
                     QKKLKPLADPGEEDLAEILYTSGTMGHSKGVMLTHANIVSNVQAGIQAISISHSDCFL
                     AILPLSHAYQCSMGFLAPFLMGSPIYFIKGLPTAQTLLPAIKEVQPTIILSVPLIMDK
                     IYKKKVLGEINAKPISKTLYRIGPMQKVVNRIAGRRLVQAFGSRLRLMIFGGAATPPD
                     VEAFLHEGGFPYTTGYGLTECSPLLTVNPIGKVKLQSAGKAIPGVTLRILDPDPQSGI
                     GEIIARGPNVMKGYFHNEEATRKAFTAEGWFITGDLGLIDSDGYLFIKGRSKNIIVGP
                     SGENIYPEELEFHLSQNPCVLESLVYEQAGRITARVYLDMDAMEEAYHLSRLDGSEAA
                     RIVTQKLESIRQEVNSKVAAYARIHRIIEQAEEFEKTATKKIKRYLYIN"
     gene            complement(4933..5280)
                     /gene="rplS"
                     /locus_tag="PKM23_08935"
     CDS             complement(4933..5280)
                     /gene="rplS"
                     /locus_tag="PKM23_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNY91637.1"
                     /translation="MNKLDLVAASQMKTDIPAFHPGDTVNVHVKVIEGDKERIQVFQG
                     VVINRKGAGINATFTVRKISEGIGVERIFPLHSPNIAKIENIREGKVRRAKLYYLRSL
                     RGKAARISEKRSQ"
     gene            complement(5314..6012)
                     /gene="trmD"
                     /locus_tag="PKM23_08940"
     CDS             complement(5314..6012)
                     /gene="trmD"
                     /locus_tag="PKM23_08940"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNY91638.1"
                     /translation="MEIHVVAAFPGLLESALGDSILKRAQSKGLVRIHFHEIRPYATD
                     KHQQIDDYGFGGGPGMILKPEPLFRCLEDIYDRYKLEGVPVTLMSAAGRLFDQRQAVI
                     FSLREKMVLLCGHYKGIDERVIQSWVTEEISIGDYILTGGEIAATVIIDAVVRLLPGA
                     ISDIDSAETDSFQSGLLDHPHYTRPQDFRGMEVPPVLLSGNHAGISEWRLQQSLERTR
                     RLRPDLYEKYNQQD"
     gene            complement(6024..6566)
                     /gene="rimM"
                     /locus_tag="PKM23_08945"
     CDS             complement(6024..6566)
                     /gene="rimM"
                     /locus_tag="PKM23_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNY91639.1"
                     /translation="MADQSGKRARTPDFVVIGQVRRPHGIRGALRVAPLTDDPERYHL
                     LKRVWLNHGDDERTPFTLAQVQLIPDGIILSLDGISDRNAAETWRQAWVEIPGEEVLP
                     LAEGRHYIFEIIGVQVVTEEGVALGEVVDILRNPAHDVYVVRGDEREYLIPAVPEFIR
                     QIDSETGLMIVHVVDGLLDL"
     gene            complement(6590..7216)
                     /gene="rpsP"
                     /locus_tag="PKM23_08950"
     CDS             complement(6590..7216)
                     /gene="rpsP"
                     /locus_tag="PKM23_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNY91640.1"
                     /translation="MSVKLRLSRIGKKKQPFYRIVAIDSRSSRDSSYLDKIGHYNPVT
                     QPAELVIDRAKALKWLSDGAIPSDTVNSLFRSQGILHEWEMHKRGANAETIAEEMTRW
                     QDLQLQRSKREEAKQAMEKRETETKKPAKSEAKAPAAEAPAPAPVEAPAAAPAAEAPA
                     PAPVEAPAAAPVAEEPPAEARAPEEPAAEAPAAEEPAPEESAEPKSEA"
     gene            complement(7231..>7672)
                     /locus_tag="PKM23_08955"
     CDS             complement(7231..>7672)
                     /locus_tag="PKM23_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNY91641.1"
                     /translation="GMGDVLSLVEKAQEAVDLEKAVKLEKKLRRAEFNLEDFLDQLQQ
                     LKRMGPLQDILTMIPGAQAKAFKGMQVDDKALIRVEAIIRSMTVKERNNPNILDGSRR
                     KRIARGSGTSVQEVNRVLNQYNMIVKMMKTMKHGGRGMRLPTGF"
BASE COUNT         1530 a         2284 c         2269 g         1589 t
ORIGIN      
        1 cgggggtccg ccgatctgat cacaacggac ggcagcatct cgatcggcag cgtcgagggc
       61 gccgtcgcag cccgcacatc aggcggcggc atcagcctcg gctccgccac cggccgtgcc
      121 cagctcgcca cctcaggggg gagcatctcc gtcgatgcgg tgaagggggc gatcgaagcc
      181 tccacctccg gcggagggat cacggccgct atcaccgcac aaccccagca gccctgccgg
      241 ctcgccacct ccggcggcag catcaaggtc cacctggcgc ccgatctccg cctggacatc
      301 aatgccagca ccagcggggg acacgtgcac agcgagtttg acctcctcat ccacggcggg
      361 atgagcagat cctccctcca gggcaagctc aacggcggcg gacccgaact ttatctgcgc
      421 acctccggag gcaacatcac cctcgccaaa ccggagctgt aatccggcgg aattgatcag
      481 ccattaatta catttttgta attcacagca cggcgccgtt ccggcggcgc cgtgcgtccg
      541 cgaacgcccc cgagcgtgac ccctgtcaaa tttttggaca ttttcccccc gccgcagcaa
      601 caattgcttg cgattctccc gatttgtatt atatttacta ttgatatagc gacgggggtg
      661 acgagcgatc ctccccctcc atccgatccg cctttcccgc acatctctct cgctccggca
      721 actctctttc aagttacacg attggaggta caatgaaaaa agcgcttctt ctcatcgctc
      781 tgattgccct ggcgggctgc agcaagcaga tgatccggtt cgatcaatac tcggtggcca
      841 tgaacctcac ggtcgatgcg gacagctcgg tctacctggg tgatggagat aaattcaacg
      901 gcgtcctctt tctcgccccc atcctcaggg aggaaaacca gccggtctcc acagtcaagg
      961 tgatccagaa ctatggccgc tattacctct gcgccgatga gttcaggaat ctctggatga
     1021 tcgagcccac ctcggacgga accgaaggca agatcaaggc cattgatgtg accccggagg
     1081 atgaaaccga tcaactccgg aacatcagcc tctcccgcta cggcaccgag gagaaggcct
     1141 gcatccgctt ccgcttcaac ggcaaggaaa tattcatcaa tcagaaaggt ggtttgaatg
     1201 aagagtgcaa atagctggat gatgctcttc ctgctcctcc tcgccccgct ccttcaggcg
     1261 caggatgtgc aggatcttta cgagcaggcc aaggaggcgc tcaggcaagc ggattaccag
     1321 acctgtctgg acaagatcgc cgccgccaag gcggagatca gtcaggatcc ccagctggat
     1381 ccgaacggcg tctttatgaa acggctgctg cccgaggtgg agcgcaacgg ccagaatatg
     1441 gccgccatca tgctggccct ggaggagctg tatgactcct cgcaaagcgg cgtcaccttc
     1501 gccgatctcg gcctgaacca ggaggccatc acccagtatc acgcacaggc ccgcgatgcc
     1561 agcgatgatc tgcagcgccg ccgggacgag atcctcgcct cccgcgaact cgcccccgag
     1621 taccgcaccg ccctgctcag ggccccggtg ctgacccgca tcgaaaaact cgcctccacc
     1681 ggcatcatgg aaaagctgac cggcaagttc tcgcagatgg tggggctcct caccgactct
     1741 ctctccgccg tcgacaaccg cttcaggatg gcggaagccc gcctggccaa gctgcagaaa
     1801 tcggccgccg cctccaaaca ggagatcgaa aagatcaagg gcgagatggc caaactcaac
     1861 accgagcgcc tctcctatat caacaccatc tcggagatgc tcgccggcga ggccagcccg
     1921 gagatggaac ccattcgcgt cgccctctcc gaaaaccagg tggatgacgt ctttcgcggc
     1981 gtgatcgaat cggaaatccg ccgggtcaag ggcatcacca ccatcgactc ggttggctac
     2041 aaggagctgc tcaaggatta cgaccgcatc cgtaactaca accgcatctt cgccaagaat
     2101 aatatcgcgc cggatcaggc ggccctgctc gccagctacg acgccgcgat caaggcggtc
     2161 aaggtggaag tacccaagac cacaaactgg accttgatct atatcatcat cggggttttg
     2221 ctcctgctcc tgctctgggc cctctacaaa ctcttcggcg gcagcaaaaa gaagagcccg
     2281 cccccgccgg aatataaaag ctaccagccc tgatcaccct gacgggtgcg cccgcctcta
     2341 tcatctatta aaaaaagctg cgctccgaat catgcagagc gcagcttttt tctattccaa
     2401 ccgttacgaa tcgctggccg gacaggcacg aaccacgctc cgcgggagta tggacattgt
     2461 tcccggggac gacacccaac ccgctgcggg caagtaaatc cttgattatt tcataaaata
     2521 ttcctatttt atggcgaaat taagggactc catataagga aaagccatgc tcagcaaaaa
     2581 aaagatggag atcggcatag aggtcgaggc gctgtgcaac aaatgcaagg ccgccacggt
     2641 ccatgttgtg gtcgcgatca agagccagga catggtccgg gtgatgtgca aaacctgtca
     2701 gagtttgcac cgcttcaagg cgccggaatc caagggcgtc aaaaaagccg ccaaggtgca
     2761 gaaagcccaa aaggtcaaat tgaccccgga ggcgagagag agtaaaaaat ggaacaagct
     2821 gctgctcgcc gcggatccgg aaaaggcggt agcctattcg atgtccagtg tttatgccga
     2881 aaacgatatc atccaacaca ccaaattcgg ccttggtgtt gtggtgaaga tacttgatcc
     2941 gagcaaaata tcagtggtct ttgaggacgg cgtaaaaaac atggtccaga acctcaaata
     3001 gtccgccttg cttcaatgaa caaaaaaaaa ggtgcgattt cgatcgcacc ttttttttta
     3061 tgccccgacc taattgatgt aaagatagcg tttgatcttc ttggtcgctg tcttttcgaa
     3121 ctcctcggcc tgctcgatga tgcggtggat gcgtgcgtag gccgctacct tgctgttgac
     3181 ctcctgtcgt atcgactcca gcttttgtgt cacgatgcgg gccgcctccg agccgtccag
     3241 cctggagaga tgataagctt cctccatggc gtccatgtcg agatagaccc tggcggtgat
     3301 gcgcccggcc tgttcgtaga ccagggattc gagtacgcag ggattctggg agagatggaa
     3361 ctcgagttcc tcgggataga tgttctcgcc gctcgggccg acaatgatgt tcttcgacct
     3421 ccccttgatg aagagatagc cgtcgctgtc gatgagtccg aggtcgccgg tgatgaacca
     3481 gccctccgcg gtgaaggcct tgcgggtcgc ctcctcgttg tggaaatagc ccttcatgac
     3541 attggggccg cgggcgatga tctcgccgat gccggattgg gggtctggat cgaggatccg
     3601 cagcgtcacc cccgggatcg ccttgccggc ggactgcagc ttgaccttgc cgatgggatt
     3661 gacggtgaga agaggcgagc actcggtcag gccgtagccg gtggtgtagg gaaagccccc
     3721 ctcgtgcaag aaggcctcga catccggcgg ggtggccgcg ccgccgaaga tcatcaggcg
     3781 cagccggctg ccgaaggcct gcaccagccg cctgccggcg atgcggttga ccaccttctg
     3841 catcggtccg atgcggtaga gggtcttcga gatcggcttg gcgttgatct caccgagcac
     3901 cttctttttg tagatcttgt ccatgatcag gggcaccgaa aggatgatgg tcggctgcac
     3961 ctccttgata gccggcagga gggtttgcgc cgtgggcagg cccttgatga agtagatcgg
     4021 ggagcccatc aggaaggggg cgagaaaacc catactgcac tggtaggcat gcgacagggg
     4081 caggatggcc aaaaaacagt cgctgtggct gatggagatc gcctgaatgc cggcctggac
     4141 attactgacg atgttggcat gggtcagcat cacccccttg gaatggccca tcgtgcccga
     4201 ggtgtagagt atttcggcga gatcctcctc cccgggatcg gcgagcggct tgagcttttt
     4261 ctgcttcctg gcgggcgcct caccggccag cagcgcggaa agtcttaccc actttttctc
     4321 atccaggggg agttcctgat tgtcgaggga ggcgacaaag acaagatcgc ggatattgag
     4381 ttcgaccacc ttgtggacga gtttgctggt gacaaagagt cccctggcgt ttgagttgcg
     4441 gatgatatgg tggatttcgg atttgtggaa ctcggtgagg atgggcacga ccacggcccc
     4501 catgctgacg atcgccagat aggcgatgca ccagctcggg gaattgtcgc cgaggagggc
     4561 gatgcggtcg cccggccgga tgccgcgctg gcgcaggctc tggacgagct ggtggacctg
     4621 ctgttcgacc tggcgatagg tatagggttc ctcgccgacg aaggagaaga cgatatcgcg
     4681 ggaaaattcc gccaccccgg cgcgaaatag ttcgcagagc gtccgtttct gcagcgcaac
     4741 catatgggaa ctcctgatga tgtactcttg tccccggccg gcggggaatt gatgcagccc
     4801 gaaaaggggg caggaaaagg aaatataaca aatttccggc aacctgtcaa tatctttgtg
     4861 gtggatgcat ataaaaatga ccgccctccg cgggggcggg cggtcatgca aagctggcgt
     4921 gtggatggag ggttactggc tgcgtttttc gctgatgcgg gcggccttgc cgcgcaggct
     4981 gcgcaggtag tagagcttgg cgcggcgcac cttgccttcg cggatgttct cgatcttggc
     5041 gatgttcggc gagtgcagcg ggaagatgcg ctccacgccg atgccttcgg atatcttgcg
     5101 gaccgtgaag gtggcgttga tgccggcccc cttgcggttg atcacaacac cctgaaaaac
     5161 ctggatgcgc tccttgtcac cttcaatgac cttgacgtgc acattgaccg tgtcgcccgg
     5221 atgaaaggca gggatgtcgg tcttcatctg ggatgcagca acgagatcca acttgttcat
     5281 tttgttttcc tcctctttgc gagggtttat gtttcaatcc tgctgattat acttttcgta
     5341 gagatcgggc cgcagacgcc tggtccgttc caggctctgc tgcaggcgcc actcggaaat
     5401 cccggcatgg ttcccggaga gcagcaccgg cggcacctcc ataccgcgaa aatcctgcgg
     5461 ccgcgtgtag tgcggatggt cgagcagtcc tgactggaac gaatcggtct ctgcggaatc
     5521 gatgtcgctg atcgcgccgg gcagcagcct gaccacagca tcgatgatga ccgtggcggc
     5581 aatctccccc ccggtgagga tatagtcgcc gatcgaaatc tcctcggtga cccagctctg
     5641 gatgacgcgc tcgtcgatgc ccttgtaatg cccgcagagc agaaccatct tttcgcgcag
     5701 ggagaagatc acggcctgcc tctggtcgaa gagccggccg gccgccgaca tcagggttac
     5761 cggcaccccc tccagcttgt acctgtcata gatatcttcg aggcagcgaa agaggggctc
     5821 cggtttgagg atcatgcccg ggccgccgcc gaaaccgtag tcgtcgatct gctgatgttt
     5881 gtcggtcgcg tagggccgga tctcgtgaaa atggatcctg accagccctt tggactgcgc
     5941 ccgcttgagt atgctgtcgc ccagggcgct ctcgagtaat ccggggaaag cggctacgac
     6001 atgaatttcc acccgttact ccgttacaaa tccagtaagc catccacgac gtggacgatc
     6061 atgaggccgg tctcgctgtc gatctggcgg atgaactccg gcacggccgg aatgagatat
     6121 tcgcgctcat cgccgcgcac cacatagaca tcgtgggccg gattgcgcag gatgtcgacc
     6181 acctcgccca gagctactcc ctcctcggtc accacctgaa ccccgatgat ctcgaaaatg
     6241 taatgccggc cttcggcgag agggagaact tcttcccccg gtatctcgac ccaggcctgg
     6301 cgccaggtct cggcggcgtt gcggtcgctg atcccgtcga ggctgaggat gattccgtca
     6361 ggaatgagct gcacctgggc gagggtgaag ggggtgcgct catcatcgcc gtggttgagc
     6421 cagacccttt tgagcaggtg atagcgctcc gggtcatcgg tcaagggcgc gacgcgcaag
     6481 gccccgcgga tgccgtgagg ccttctcacc tggccgatga ccacaaagtc cggggtcctg
     6541 gcccgtttgc cgctctgatc cgccattaca gatgatgggt tgtcgtcgct caggcttcgc
     6601 tcttgggctc ggcgctctct tcaggcgcag gctcctcagc cgctggagcc tctgctgcgg
     6661 gctcttcagg tgcacgagct tcggccgggg gctcttcagc tacaggggca gctgcaggag
     6721 cttcgaccgg ggcgggagct ggtgcttcgg ccgccggggc tgctgcagga gcttcgaccg
     6781 gggctggagc cggtgcttcg gccgcggggg ctttggcttc gctcttggcc ggtttcttcg
     6841 tttccgtctc gcgcttctcc atcgcctgtt tggcttcctc gcgcttgctg cgctggagct
     6901 gcagatcctg ccagcgggtc atctcttcgg cgatggtctc agcgttcgcc ccgcgcttgt
     6961 gcatctccca ctcgtggagg atgccctggc tgcggaaaag gctgttcacc gtatcggagg
     7021 ggatggcgcc gtcgctgagc catttcaggg ccttggcgcg gtcgatgacc agttcggcag
     7081 gctgggtgac gggattataa tgtccgatct tgtccagata ggagctgtcc cgtgaggatc
     7141 tcgagtcgat ggcgacgata cggtaaaaag gctgtttctt cttaccaatg cggcttaacc
     7201 ttaactttac cgacaacttg ctctcctttg ttaaaagccc gtgggcaggc gcatgcccct
     7261 gcccccatgt ttcattgtct tcatcatctt tacaatcata ttatactgat tcagaacccg
     7321 gttgacctcc tggacgctgg tgccgctgcc gcgggcgatg cgcttgcggc ggctgccgtc
     7381 taggatgttc gggttatttc tctccttgac cgtcatggag cggatgatcg cctccacgcg
     7441 gatgagggcc ttgtcgtcaa cctgcatccc cttgaaggcc ttggcctggg cgccggggat
     7501 catggtcagg atatcctgga gcggccccat gcgtttgagc tgctggagct gatcgaggaa
     7561 atcctcgaga ttgaactcgg cccggcgcag cttcttctcg agcttgacgg ccttttcgag
     7621 gtcgaccgcc tcctgcgctt tttccaccag ggagaggacg tcgcccatgc cg
//
LOCUS       DAOPWU010000157         7669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9543_length_7669_cov_4.644208, whole genome shotgun sequence.
ACCESSION   DAOPWU010000157 DAOPWU010000000
VERSION     DAOPWU010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9543_length_7669_cov_4.644208"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PKM23_08960"
     CDS             <1..956
                     /locus_tag="PKM23_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91642.1"
                     /translation="GRIRFFLKENPHRGIWHTQQVARNGLIHAVLCGTEFENRGYRYW
                     GEPAYEQWIGSGSQLLPALAIPGRAAAALTGSRFITMIDSLSPEVREEAIWQELRLGN
                     LPDFLRRPVLLERKWADAAGETHQVVFAALPDYLAIGSDADFVRMPMTPFTAQRAADL
                     FGAILPTRKLVDAIYAAAETRLTPQPIPPDGDRNERPRTFLAHQHQVEKALLMAGGVH
                     GRLTAGHKKDIVLSNLIADPQRPGHVVIYGWHRPDARPIQPLTNIHIGRYADYSHGVR
                     LICASVLVDGVPWQVERLLADPLLYALFSDEEGVMTQRRYP"
     gene            complement(997..2721)
                     /locus_tag="PKM23_08965"
     CDS             complement(997..2721)
                     /locus_tag="PKM23_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNY91643.1"
                     /translation="MERKFMTAPLSSSRYDLKSVRLPYLAGLPLRIMVWIIESPLGRL
                     LIPQLFKSAGITWLRKQSFAEAPLYTPLYRSGRAAAPRQEGMPALPPEPAPGFHFSST
                     ADYASAYREGVTDPEQTASRILDLIDASDRGQEPLRAITASYTEELLRQAQESAQRLR
                     EGRPRSIFEGVPVAVKEELDVVPYPTTVGTAFLGREAAVRDATVVARMRAAGALIIGK
                     ANMHEIGIGVTGLNPIHGTPRNPYAPGHYTGGSSSGPAAAVAAGLCPVAIGADGGGSI
                     RIPSAFCGIVGLKPTFGRVSEIGAWPLCWSVAHVGPLAASVADAALAYGVMAGPDEKD
                     PNTLHQPEVEWSGWDQFDLSDLTLGIYPEWFRHAQPAIVAACEAQIERFTALGARVVE
                     IVLPDLEAARVAHTITIAAEMAQSMEADYRQNHRLHGRDVRLNLALARAMSARDYILA
                     QRVRTRLVRHLEHAFSKADLILSPATGITAPPILPSALKGGESDLTKLVEIMRFATPG
                     NLAGVPAISFPAGYDPEGLPIGMQAMGPAWQEKKLLRLALAAERGLERRQPQRHFPIL
                     PVPEIASA"
     gene            2885..6049
                     /locus_tag="PKM23_08970"
     CDS             2885..6049
                     /locus_tag="PKM23_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNY91644.1"
                     /translation="MNKFLLCCSLFCLFASLDQAWSQLPDWENPAVFSINKEAPHATY
                     APHSEMAAAITNERMASPWMQSLNGPWKFNWVSKPADRPLDFYKPGFDASSWKEIQVP
                     SNWELQGYGQPIYVNIPYEWTKDPKPPKVPHDLNPVGSYLRTFTVPEAWAGREVFIHF
                     GAVKSAFYIWINGEMAGYSEDAKTPAEWNITKYLQPGLNSVALEVYRWSDGSYLECQD
                     FWRISGIERDVFLFSTPKTRIRDFWARAELDSLYHDGLLSIDVELLGVEVRKKRDPHT
                     IEAALLDAQGNAIYQAAQTIEMKGKTAATVTFAQRIPAPRQWSAESPNLYSLVLTLKD
                     AKGTATERVGCKVGFRTVEIRDGLLLVNGAAVLIKGVNRHEHDEHTGHVISEASMVRD
                     IQLMKQNNINTVRTCHYPNDPRWYELCDQYGLYVIDEANIESHGMGYKPERTLGNHPD
                     WIAVHVDRVSRMVERDKNHPSVIIWSLGNEAGDGVCFTAAYNWVKSRDLSRPVHYERA
                     EMRSNTDIYCPMYEHIDNMLDYASKPQSRPLIQCEYAHSMGNATGNLQDYWDVIEAHP
                     QLQGGCIWDWVDQGFARTTPDGRKYWAFGGEWGPPSDANFCCNGLVCPDRTPHAALQE
                     VKKVYQSIKFKAVNLSDGEIEIRNGYSFIPLSGFEFVYRLELDGRTIAGEALPPLDLA
                     PGQSTRLRIPLPILTVLPHAGYFLTLEARTREEAPMVPKGHVMAAEQFELPLPEVAVE
                     RSKAAGPALKLKESKQTLDIQGANFSVRFDKGTGLLTSFISGSRELLHTPPAPNYWRP
                     PTDSDFGNGMNKRCLPWRTASCERKLITLQTTPVKVKKTGLIAVQVDVFYELPAVGRE
                     QIRYTVLGEGEIQVERHFTAMEEKLPEMPRFGMALAMPAGFERVRWFGRGPHENYSDR
                     KTSAFVGLHELTVDGFNEPYVSPQEMGYRTDTRWLAVTDAQGSGLLFIGPLCFSALHY
                     TNEDLTQKSRGSIHPTDLTRRDEVCLNIDLAQMGVGGDNAWGAWPHKQYLLQEKKYSH
                     KFVMRPLAAGDDPGERSRGRILLP"
     gene            complement(6087..6944)
                     /locus_tag="PKM23_08975"
     CDS             complement(6087..6944)
                     /locus_tag="PKM23_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91645.1"
                     /translation="MRRIGALLLLVIAGLSGCSRQMPPGRVAAWLPDQPQAGGEITLF
                     YNPAVPGAVLVNETGLTAEMLIMRTSEPPILTELPMTQKDGVWRTHHLLPAEGQFFLL
                     RFAANGKTDDNGGRCWEGILHGREMRPLRGAHLQKSLLLQQGGAGGFPFRQSLSEAET
                     ELEAELALYPDNLEAKTALWDLLLRRSPLASTKERVRNELRQIYENAGGDEETLAALL
                     PFFFRTGQEYAARQIIAESVALLPRGPVAAAARRREIEREQDPIKKASMIEAYNKEFP
                     QQGLWRQLP"
     gene            complement(7077..>7669)
                     /locus_tag="PKM23_08980"
     CDS             complement(7077..>7669)
                     /locus_tag="PKM23_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91646.1"
                     /translation="DPVQNGKIHFIGKGEICVVGSKDGGAKWGEPVNITETPTFDERN
                     VVLNKKVVDGKLHMVYFGDQGAGWEYINYLSNPYLSGVYYLALPVSGIPTGVKDRPVE
                     VVESYELAQNYPNPFNPTTTIDFSVPKASQVKLTVYNMLGQKVKTLVDQSMTAGKHSI
                     KWNGTDESGNMVTSGMYIYRIEGQFGVKSHKLMFLK"
BASE COUNT         1451 a         2393 c         2391 g         1434 t
ORIGIN      
        1 ccgggcgtat ccgtttcttc ctgaaggaga atccgcaccg cgggatctgg cacacccagc
       61 aggtggcgcg caacggcctg atccatgccg tactgtgcgg aaccgaattt gaaaatcggg
      121 gataccggta ctggggcgag ccagcctacg aacaatggat cggctcgggc agtcagctcc
      181 tgcctgccct ggctataccc gggcgcgccg cggctgccct gacgggctcc cggtttatca
      241 caatgatcga ctcgctctcc cccgaggtgc gcgaggaggc catctggcag gagctgcgct
      301 tgggcaacct ccccgatttc ctgcgtcgtc ccgtcctcct cgaaagaaaa tgggctgatg
      361 cggccgggga gacccatcag gtcgtgtttg ccgcgttgcc tgattatctg gcgatcggat
      421 cggatgccga tttcgtccgc atgccgatga cgccctttac cgcgcagaga gcggccgacc
      481 tcttcggagc cattttgccc acccgcaagc tggtggatgc catttacgct gcagcagaga
      541 ccaggttgac gccgcagccc attccgcccg atggggaccg caacgaacgg ccccggacct
      601 ttctggcgca ccagcatcag gtcgaaaagg ccttgcttat ggctggaggc gtgcacggcc
      661 ggctcaccgc cgggcacaag aaagatatcg tgttgagcaa cctcatcgcg gatccccagc
      721 ggccggggca tgtcgtcatc tacggctggc accgcccgga cgccaggccg atccagccct
      781 tgaccaacat ccacatcggc cgttacgccg attacagcca tggcgtccgg ctgatctgcg
      841 cgtcggtgct cgttgacggt gtgccctggc aggtggagag gctgcttgcc gatcccttgc
      901 tctacgccct tttcagtgac gaagaggggg tgatgacgca gaggcgctac ccctgacctg
      961 accgctgcgc ggtgttctct cttcccgggc cggtcttcag gcggaggcta tctccgggac
     1021 gggcaggatc ggaaagtggc gctgcggctg ccggcgctcc aggccgcgct cggccgccag
     1081 ggccaggcgc agcagcttct tctcctgcca ggccgggccc atggcctgca tgccgatggg
     1141 cagcccctcc ggatcgtatc ctgccgggaa ggagatcgcc ggtacgccgg cgaggttgcc
     1201 cggggtggcg aagcgcatga tctcgaccag tttggtcagg tcggactctc cgcccttgag
     1261 ggcactgggc aggatcggtg gcgcggtgat gcccgtcgcc ggggagagaa tgagatcggc
     1321 cttgctgaag gcgtgttcca gatggcggac cagacgggtg cgcacccgct gggccaggat
     1381 atagtcgcgt gcgctcatcg cccgggcgag ggcgagattg aggcggacgt cccgcccatg
     1441 caggcggtgg ttctgccggt aatcagcctc catggattgg gccatttccg cggcgatggt
     1501 gatggtatgc gccacccgcg ccgcttccag atcggggagg acgatctcca ccacccgcgc
     1561 tcccagtgcc gtgaagcgct cgatctgggc ctcgcatgcg gcgacaatgg ccggctgggc
     1621 atggcggaac cattccggat aaatccccag ggtgagatcg ctgaggtcga actggtccca
     1681 gccgctccac tccacctccg gctggtggag ggtgttggga tccttttcgt cggggccggc
     1741 catgacacca taggcgaggg cggcatccgc gacgcttgcg gcgagggggc cgacatgggc
     1801 gacgctccag cagaggggcc aggccccgat ttcgctgacg cggccgaagg tgggcttgag
     1861 tccgacaata ccgcaaaagg cggagggaat gcggatcgat cccccgccat cggctccgat
     1921 cgccaccggg cagaggccgg cggccaccgc ggcggccgga ccactcgagc tgccaccggt
     1981 gtaatgaccg ggggcgtagg gattgcgcgg ggtgccgtgg atggggttga gcccggtcac
     2041 gccgatgccg atttcgtgca tgttggcctt gccgatgatc agcgcccccg cggcgcgcat
     2101 gcgcgccacc acggtcgcat cccgcacggc cgcttcgcgc ccgagaaagg cggtgccaac
     2161 tgtggttggg taagggacga catccagttc ttccttgacc gccactggca ccccctcgaa
     2221 aatggagcgc ggccggcctt cgcgcaagcg ttgggccgac tcctgtgcct ggcgcaaaag
     2281 ctcttcggta tagctggccg tgatcgcccg caggggctct tggccgcgat cactggcatc
     2341 gataaggtcg agaatgcggg aggcggtctg ttcgggatca gtcaccccct cgcgataggc
     2401 gctggcatag tcagccgtgc tgctgaaatg aaaaccgggg gcgggctctg gcgggagcgc
     2461 aggcatgccc tcctgccggg gagcggcggc ccgaccgctg cggtagaggg gagtatacag
     2521 gggagcttcg gcaaaactct gcttgcgcag ccaggtgatg ccggctgact tgaagagctg
     2581 ggggatgagc agcctgccga gggggctttc aattatccaa accatgatcc ggagcggcag
     2641 gccggcgaga tagggaagcc gcaccgattt cagatcatac cgggaggagg agaggggagc
     2701 agtcatgaac ttcctttcca tcgatttaaa gtggcccccc ggggtaaatc tccctgaata
     2761 taggggattt tcaccattac cgccataaaa aaatgatttt cttttcaaaa gaaaaaccat
     2821 acctttagag gccgcgggac cggcagaggt ccgcggtcca tcatataatg aaaacgaggt
     2881 cgagatgaac aagttcctgc tctgctgctc cctgttctgt ttattcgcca gcctggatca
     2941 ggcctggtca cagctgcctg attgggagaa tcctgccgtt ttcagcatca acaaggaggc
     3001 tccgcacgcc acctatgcac cccacagcga aatggctgcc gccatcacca acgagcgcat
     3061 ggcttcaccc tggatgcaga gcctcaacgg tccctggaaa ttcaactggg tgagcaaacc
     3121 cgccgaccgg cccctcgatt tctacaagcc cgggttcgac gccagcagct ggaaagagat
     3181 ccaggttcct tcgaactggg agctgcaggg ttacggccag cccatctatg tcaatatccc
     3241 ctatgaatgg accaaagatc ccaaaccccc gaaggtcccg cacgacctca atccggtcgg
     3301 ctcctacctg cgcaccttca ccgttccgga agcatgggcc ggccgcgagg tctttatcca
     3361 cttcggcgcg gtcaagtccg ccttctatat ctggatcaac ggcgagatgg cgggctacag
     3421 cgaggatgcc aagaccccgg cggagtggaa tatcaccaaa tatctccaac ccggcctcaa
     3481 cagcgtcgcc ctcgaggtct accgctggtc ggatggctcc tacctcgagt gccaggattt
     3541 ctggcgcatc agcggcatcg agcgcgatgt ctttcttttc agcaccccga agacgcgcat
     3601 ccgcgatttc tgggccagag cggaactcga ttccctctat catgacggcc tgctctcgat
     3661 cgacgtcgag ctgctcggcg tggaggtgag gaaaaaaaga gatccccaca ccattgaagc
     3721 tgccctgctc gacgcacagg gaaacgccat ctatcaggcc gcgcaaacca tcgagatgaa
     3781 ggggaaaacc gccgcgactg tcacctttgc ccagcgcatt ccggcacccc ggcagtggtc
     3841 ggccgagagc cccaatcttt acagcctggt cctgaccctc aaggatgcca aaggcacggc
     3901 gaccgaacgg gtcggctgca aggtcggctt tcgcacggtg gagatcaggg atggactgct
     3961 gctggtcaat ggcgccgcgg tgttgatcaa gggggtcaac cgccatgagc acgacgaaca
     4021 caccggccac gtcatctccg aggcctcgat ggtgcgcgac attcaactca tgaagcagaa
     4081 caacatcaat actgtgcgca cctgccacta tcccaacgac ccgcgctggt acgaactctg
     4141 cgaccagtac ggcctctacg tcatcgacga agccaacatc gaatcccacg gtatgggcta
     4201 taaacccgag cgcaccctcg gcaaccatcc ggactggatc gcggtgcatg tggaccgcgt
     4261 cagccgcatg gtcgaacgcg acaagaatca tccctcggtg atcatctggt cgctcggcaa
     4321 cgaggccggc gacggggtct gcttcaccgc cgcctacaac tgggtgaaat cgcgagatct
     4381 ctcgcgcccg gtccactacg agcgtgcgga gatgcgcagc aataccgata tctactgtcc
     4441 gatgtatgag cacatcgaca acatgctcga ctatgcctcc aaaccgcaga gccggccgct
     4501 catccagtgc gaatatgccc actcgatggg caacgccacc ggcaacctcc aggattactg
     4561 ggatgtgatc gaggcccatc cccagctcca ggggggctgc atctgggact gggttgatca
     4621 gggcttcgcc cgcaccaccc cggatgggcg caagtactgg gccttcggcg gcgaatgggg
     4681 cccgccctcg gacgccaatt tctgctgcaa cggcctggtc tgcccggacc gcactcccca
     4741 tgccgctctg caggaggtca agaaagtcta ccaatcgatc aagttcaagg cggtgaatct
     4801 gtcggatggc gaaatcgaga tccgcaacgg ctacagcttt attcccctga gcggcttcga
     4861 attcgtctac cggcttgaac tggacgggcg gaccatcgcc ggggaggcgc tgccacccct
     4921 cgatctggcg ccgggccagt ccacccgcct gcgcattccg ctccccatcc tgaccgttct
     4981 tccccatgcc ggctatttcc tgaccctcga ggcgcgcacc cgcgaggaag cccccatggt
     5041 ccccaaagga catgttatgg ccgctgagca gtttgagctg cccctgccag aagttgcggt
     5101 ggagcgcagc aaggcggcag gacccgctct gaagctgaag gagagcaagc aaaccctgga
     5161 tattcaaggg gcgaatttca gcgtgcgctt cgacaagggc accggcctgc tgacctcttt
     5221 tatctccggc agccgggagc tcttgcacac gccgccggcg cccaattact ggcggccgcc
     5281 gaccgacagt gatttcggca atggcatgaa caaacgctgc ctgccctggc gcaccgccag
     5341 ttgtgaacgc aagctgatca ccttgcagac cactccggtt aaagttaaaa aaacggggct
     5401 catcgccgtg caggtcgacg ttttctatga gctgccggca gtcggccgcg agcaaatccg
     5461 ttatactgtg ctgggtgaag gcgagattca ggttgagcgc cattttaccg cgatggagga
     5521 aaagctgccc gagatgccgc gctttggcat ggctctggcg atgccggccg gctttgaacg
     5581 ggtgcgctgg ttcggccgcg ggccgcacga gaattacagc gatcgcaaaa cctctgcctt
     5641 tgtcggcctc catgaattga ccgtggacgg gttcaacgag ccctatgtct cgccgcagga
     5701 aatgggctac cgcaccgata cccgctggct ggcggtgacc gatgcgcagg gcagcgggtt
     5761 gcttttcatc gggccgctct gcttttcggc cctccattat accaacgagg atctgaccca
     5821 gaaaagccgc ggatcgatcc atccgaccga cctgacgcgg cgcgatgaag tgtgcctcaa
     5881 catcgatctg gcccagatgg gggtgggcgg ggataacgcg tggggggcat ggccgcacaa
     5941 gcagtacctg ctgcaggaga agaaatacag ccacaagttt gtgatgcggc cgctggcggc
     6001 cggggatgat ccaggggagc gcagccgcgg ccggattttg ctgccctgag caggcgccgg
     6061 ccctgttttg acgcggcagc cccctgctag ggcaattgcc gccacagacc ttgctgtgga
     6121 aactctttgt tataggcctc gatcatcgag gcctttttta ttggatcctg ctcccgttcg
     6181 atctcccggc gccgcgctgc tgcagcgacg ggtccgcgcg gcagcagggc gaccgactcg
     6241 gcgatgatct ggcgggcggc gtactcctgg ccggtgcgaa agaagaaggg gaggagggcc
     6301 gccagggtct cctcgtcgcc gccggcgttt tcatagatct ggcgcagctc attacgcaca
     6361 cgttctttgg tcgatgcgag gggcgaacgg cgcagcagca gatcccacag tgctgtcttt
     6421 gcctcgaggt tgtcgggata gagcgccagt tccgcctcta gttcggtttc cgcctcgctc
     6481 aacgactgcc ggaaaggaaa ccctccggca cctccctgct gcagaaggag gcttttctga
     6541 agatgagcgc cgcgcagggg gcgcatctct cggccgtgga ggatgccttc ccagcagcgg
     6601 ccgccgttgt catctgtttt gccgttggcg gcgaagcgga gcaggaagaa ttggccctcg
     6661 gccggcagga ggtggtgagt ccgccagacc ccgtcttttt gggtcatcgg cagctctgtc
     6721 aggatcgggg gttcggaggt gcgcatgatc agcatctctg cagtgagtcc tgtttcattc
     6781 accagcaccg ccccgggcac ggccggattg taaaagaggg tgatctcccc gcccgcctgc
     6841 ggctggtcag gcagccaggc ggcgacccgg ccggggggca tctgccggga acaacccgaa
     6901 agtccggcga tcaccagcag caacagcgct ccaattctcc tcatggcatc ctctcctgtt
     6961 tggctctccc attttaggaa atttgcgcat cagcggcaag ggaaaaagcg cggcggcgtc
     7021 ataaaaaaaa gggatggacc ggatggtcca tccctttcgg gccgaaaagc gccgcgttat
     7081 ttcaggaaca tcagtttgtg gctcttgacg ccaaactggc cttcgatgcg gtagatgtac
     7141 atgccgctcg tgaccatgtt gccgctttca tccgtaccgt tccacttgat cgagtgcttg
     7201 ccggcggtca tcgactggtc gaccagcgtc ttgaccttct gacccagcat gttgtagacc
     7261 gtcagtttga cctgagaagc cttgggcacg ctgaaatcga tcgtggtggt cgggttgaag
     7321 gggttcgggt agttctgagc cagctcgtag ctctcgacca cttcgaccgg acgatccttg
     7381 acacccgtcg ggatgccgct cacaggcagt gccaggtagt agactccgga gaggtaggga
     7441 ttgctgaggt agttgatata ctcccagccg gctccctgat cgccaaagta gaccatgtgc
     7501 agcttgccat cgacaacctt cttgttgaga acaacgttgc gctcatcaaa ggtcggggtt
     7561 tcggtgatgt tgaccggctc gccccacttg gcgccgccat ccttggaacc gacgacgcag
     7621 atctcgccct tgccgatgaa gtggatcttg ccgttctgga caggatcgt
//
LOCUS       DAOPWU010000158         7666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9550_length_7666_cov_4.384312, whole genome shotgun sequence.
ACCESSION   DAOPWU010000158 DAOPWU010000000
VERSION     DAOPWU010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9550_length_7666_cov_4.384312"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="PKM23_08985"
     CDS             complement(<1..1514)
                     /locus_tag="PKM23_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HNY91647.1"
                     /translation="MLKGHPKGLLVAFFANMGERFGFYTMVAIFILFLQAKYGMNAAA
                     ASQVYGIFMFFVYFFPLLGGIIADRVLGFSKTISIGLVVMFVGYMMLAIPSPMNQGFG
                     LVVSALAVIALGTGLFKGNLQALVGKMYEPPEYAAKRDRAFSIFYMGINVGAMLAPTA
                     AEMISNWILAKKSFFYDAHIPALANQFLNGELKDPGHYLSIAQAQDASVTLSTLGNFS
                     QAYINQLSQSYHYAFSIACFSLIISMLIFWLFRKYYKDADYMVGSQSKQAQPAHVETL
                     TPTQTRDRLIALGLVYFVVIFFWMSFHQNGVTMTYFARDYTQPSVGKFTNLWFDLFGL
                     LPIFFAAIGGYFTLRKSSSPKLRIGGLVGAVFFLILAWLRYRSYGEVNPFTPQKFQHF
                     NPFFIVALTPVVVAIFSWLNTRGKDPSAPRKIGIGMLITAAGFLILVLSSIGLPSPKE
                     LSGQVAPAASLVSPYWLISTYLTLTIAELFLSPMGISFVAKVAPPKYKGLMQGG"
     gene            complement(1633..3885)
                     /locus_tag="PKM23_08990"
     CDS             complement(1633..3885)
                     /locus_tag="PKM23_08990"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNY91648.1"
                     /translation="MEKIQVPIEGMTCAGCAARVEKRLKSLPGLSEVAVNIATGKATF
                     TLSGEGTGLAEAAAAVADAGYKMVLPAPAAAPLSADAPRERDETGESLRRDLRTALLF
                     AVPVFLISMGMELPFFQRRWPLSHETTAVLLFLLTTPVMFLPGARFFKIFWRNLRHFT
                     ADMNSLVAVGTGAAYGYSTLAALFPQLLGGSAARHIYFDSAAVIITLILLGRWLEHRA
                     KQRTGESIRALLALQPQNTAVRRGGVIVEISLGDVIPGDRVVVRPGEKIAADGLIVAG
                     ASAVDEAMITGESLPVDKKPGDRVTGGTLNTTGALEFTVTAAGGDTVLARIIRLVEEA
                     QGSKAPIQRLADRIAAVFVPVVVLIALLTLAAWMIIAGASLDRALVPFVAVLIIACPC
                     ALGLATPAAIVVGTGAGARQGILIKNGESLETAHKIDTLLIDKTGTITRGVPELSAVA
                     AGNLTEYELLRLAASVEQMSEHPLARAVAAAAQERGISLMPCEEFISHTGAGVSGRIG
                     GRLVRIGREEFMATGGIERSAWRERAERWSGEGKSLLYVAIDGRIEGLLAVADTIRPE
                     SAGAIQALRRRGIRVVMLTGDHPAAAAAIAAEAGVDDFIAGVHPEEKAEIVSREQRAG
                     RVVAMVGDGINDAPALARADIGIAIGGGADVAVESAAITLLGGDLSRVVKAIDLSRRT
                     LRTIKQNLFWAFIYNIVGIPLAALGLLNPMVAALAMSFSSVSVLANSLRLRFEPGLSG
                     AKKESAAKKE"
     gene            complement(3991..5892)
                     /gene="sppA"
                     /locus_tag="PKM23_08995"
     CDS             complement(3991..5892)
                     /gene="sppA"
                     /locus_tag="PKM23_08995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNY91649.1"
                     /translation="MEQMPPYSATQQQTMPAAARRKSRWWIVLLILFLLFVFGVIFFM
                     VGLLALVKGPFSEKPVEIKPGSVVELRVRGQLGENLQTAPLSLLGTETQSATFLELIN
                     GIQRAATDDRIRGLYYRSGDLLCGMAKAVEIRDALIKFKESGKFFKAYLDIGGELDYF
                     FASAADSIFMPTEGMLELNGFAIEDLFWKETLDKIGVQFYVEQFEEFKSAGESYSRTG
                     FSAPARQSLREILIQRQQILLQAVAAGRKLEPAMVSAAFNRGLYSSDSLLELGFIDAI
                     RSEGSVRDHLLTRESEQDSSKKIPLISMAHYARSADKSGQEVVQGKQIALIYASGVIM
                     PGEDEMIPLLDEATIASRKFVRYIRQARDDKRIKAIILRIDSPGGSVMASDEIWEEIG
                     RARKIKPVYASMSDVAASGGYYIAMACDTLIAHPASITGSIGVISTIPNLSGALKKIG
                     AHTDSLKTAEGALFLNPLYPFTEKDKKKFRQLSANIYQRFVQRVADSRGKSFEEARLL
                     ARGRVWTGEAAYTHGLVDTLGGLRTALDLAKRRIGIGADQKVRLRVFPQPQEPWDLFK
                     KLFEQLSNQNSSRSRLEAASLASTLPAWPLLPEQVRSQLEYLALLHRLGERERILAAM
                     PSIAPLAVK"
     gene            complement(5933..6730)
                     /locus_tag="PKM23_09000"
     CDS             complement(5933..6730)
                     /locus_tag="PKM23_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNY91650.1"
                     /translation="MQTRSKITNILLSAVVWCIFLVLLVLWLPLTSLIWLLDRDPARY
                     RTGRFFRWLAHPIAVLGPSWRVRMAGVRIENPRRPYVVVANHQSILDIPMLALIPMEC
                     KWIAKEPLFRIPVLGWMMRMARDIPLDRSSTRSGVKALQAARNVLEAKCSVMIFPEGT
                     RSEDRRVYDFNDGAFMLAIKDQLPVLPLAIEGASDCMPKHSWIFGDPHEIKIEILPPV
                     ETTGMSKEDLPALRDRVRGDIIRQIAAWQNVSVEQVDAAGVVRPPAW"
     gene            6933..>7666
                     /locus_tag="PKM23_09005"
     CDS             6933..>7666
                     /locus_tag="PKM23_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNY91651.1"
                     /translation="MAHDRVFFRLGMILYTLTLASLGAGEAVAQELKCFILTPPEQIL
                     AGVRQVAIADFTVTSRHEIQPKPSGKKNIDKVLDTIERVAVSGKEDKPQPFADAGKKL
                     ADLLIASMVEADRGIRSVGSGFLGMKRKEGRSFQAGARTNVFVVVERQRMDQLLEELK
                     LGQSGLVDESQAAQVGRLLGVDAIVTGQINAAVKESWEAETRTTTKGSGKNKTEEKKE
                     VWCNKRIATVSATIRIVNVETGQLFGT"
BASE COUNT         1539 a         2200 c         2560 g         1367 t
ORIGIN      
        1 caccctccct gcatcaagcc cttgtacttg ggcggcgcca ccttggccac aaaagagatc
       61 cccatcgggc tgaggaagag ctcggcgatg gtcagggtca gataggtgct gatcagccag
      121 taggggctga ccagggatgc ggccggcgcc acctggccgg agagctcctt cgggctgggc
      181 aggccgatcg aactcaacac gaggatgaga aagccggcgg cggtgatcag catgccgatg
      241 ccgatcttgc gcggggccga gggatccttg ccgcgggtgt tgagccagga aaagatggcc
      301 accactaccg gggtaagggc gacgatgaag aagggattga aatgctgaaa cttttgcggc
      361 gtgaagggat tgacctcgcc atagctgcgg tagcgcagcc aggccaggat gaggaagaag
      421 accgccccga ccaggccgcc gatgcgcagc tttggtgagc tgctcttgcg gagggtaaaa
      481 taaccgccga tagccgcgaa aaagattggc agcaggccga agagatcgaa ccagaggttg
      541 gtgaatttgc cgacgctggg ctgggtatag tcgcgcgcaa agtaggtcat ggtcacaccg
      601 ttctggtgaa aggacatcca gaaaaagatg accacgaaat agaccaggcc cagggcgatg
      661 agacggtcgc gcgtctgcgt gggcgtcagg gtctccacat gggcgggctg ggcctgtttg
      721 gactggctgc cgaccatata gtccgcatcc ttgtagtatt tgcggaagag ccagaagatc
      781 agcatcgaga tgatcaggct gaagcaggcg atgctgaagg cgtaatgata ggactggctg
      841 agctggttga tgtaagcctg ggaaaagttg cccagggtcg agagggtgac gctggcatcc
      901 tgggcctggg cgatggagag atagtggccg gggtccttga gttcgccatt gaggaactgg
      961 ttggcgaggg ccgggatatg ggcgtcatag aagaaggatt ttttagccag gatccagttg
     1021 ctgatcatct cggcggcggt gggggccagc atggcgccga cattgatgcc catgtaaaag
     1081 atgctgaagg cgcggtcgcg cttggcggcg tactcgggag gttcatacat cttgcccacc
     1141 agagcctgga ggttgccctt gaagagcccg gtgccgaggg caataaccgc cagggcggag
     1201 acgaccagac cgaaaccctg gttcatgggc gatgggattg ccagcatcat atagccgacg
     1261 aacatcacta ccaggcctat actgatggtc ttgctgaaac ccaacacccg gtcggcgatg
     1321 atgccgccca gaagggggaa gaagtagacg aagaacataa agatgccgta gacctggctg
     1381 gccgcggcgg cgttcatgcc atatttggcc tgcagaaaga ggatgaagat ggccaccatg
     1441 gtgtaaaaac cgaaacgctc acccatgttg gcaaaaaagg caaccagcaa acccttgggg
     1501 tgtcccttga gcataggagc tcctttctct agtcctttgt cagccgggcc tctccagccg
     1561 tcacagcgcc gccccgctcc ccggcctttt cttcttcata gcatgaaaaa taagggcaat
     1621 ttagataaaa agctactctt ttttcgcagc gctctccttt tttgcgccgg agaggccggg
     1681 ctcgaaacgc agccgcaagg agttggccag cactgagacc gagctgaagg acatggccag
     1741 cgcggccacc atcggattga gcagtcccag ggcggcgagg gggatgccga cgatgttgta
     1801 gatgaaggcc cagaagagat tctgtttgat ggtgcgcagg gtgcgccgtg agaggtcgat
     1861 ggctttgacc acccggctga ggtcgccgcc aagcagggtg atggcggccg attcgactgc
     1921 gacatcggcg ccgccgccga tggcgatgcc gatgtcagcg cgggccaggg ccggagcgtc
     1981 gttgatgccg tcacccacca tggcgacaac gcggccggcc cgctgctcac ggctgacgat
     2041 ttctgccttt tcctcggggt gcacgccggc aataaaatcg tccacaccgg cctcggcggc
     2101 gatggctgca gccgccgccg gatggtcgcc ggtgagcatc accacgcgga tgccgcgccg
     2161 gcgcagggcc tggatggccc cggccgattc tggccggatg gtgtcggcga ccgcgagcag
     2221 cccctcaatc cgcccgtcaa tcgccacata gagcaggctc ttgccttctc ccgaccagcg
     2281 ttccgctctc tcacgccagg ccgagcgctc aattcccccg gtggccatga actcttcgcg
     2341 gccgatgcgg accagccgac cgccgatccg gccgctgacc ccggccccgg tatggcttat
     2401 aaactcctcg cagggcatga gggagatgcc gcgctcctgc gcggctgcgg ccaccgcccg
     2461 ggcgagggga tgctccgaca tctgctcgac cgaggccgcc agtcgcagca gctcatactc
     2521 ggtgaggttt ccggccgcca ctgcgctcag ctccggcacc ccgcgggtga tggtcccggt
     2581 cttgtcgatg agcagggtat cgattttatg ggcggtctcc agactctccc cgtttttgat
     2641 caatatgccc tggcgcgctc ccgccccggt gccgaccaca attgccgccg gggtggccaa
     2701 gcccagggcg caggggcagg cgatgatgag gacggcgacg aaggggacga gagcccgatc
     2761 cagcgaggcc ccggcgataa tcatccaggc ggcaagggtc agcagggcga tgaggacgac
     2821 gactgggacg aaaaccgctg cgatgcggtc ggccagcctc tggatgggtg ctttggagcc
     2881 ctgggcctcc tcgacgaggc ggatgatcct tgccagcacg gtatctccgc ccgcagcggt
     2941 gacggtgaat tccagggcgc cggtggtgtt gagcgtgccc ccggtcacac ggtcgcccgg
     3001 ctttttatcc accggcaggc tttcgccggt gatcatggcc tcgtcgacgg cggaggcgcc
     3061 agcgacgatc agaccgtccg cagcgatctt ttcaccgggc ctgaccacga cccggtcacc
     3121 ggggatcaca tcgccgaggg agatctccac gatcaccccc ccgcggcgga cggccgtatt
     3181 ctgcggctgc agcgccagca gggctctgat cgattcgccg gtccgctgct tcgcccggtg
     3241 ttccagccag cggccgagca gaatcagggt gatgatcacc gcggccgagt cgaagtaaat
     3301 atggcgcgcc gcgctgccgc cgagaagctg gggaaagaga gcggccaggg tgctgtaacc
     3361 gtaggccgcg ccggtgccga cggccaccag gctgttcatg tccgcggtga aatggcgcag
     3421 gttgcgccag aagattttga aaaagcgggc ccccggcaga aacatcaccg gtgtggtgag
     3481 aagaaagaga agtacagctg tggtctcgtg ggagaggggc cagcggcgct ggaaaaaggg
     3541 cagctccatc cccatgctga tgagaaagac cggtacggca aagaggaggg cggtgcgcag
     3601 gtcgcggcgg agggattcgc cggtctcgtc gcgctcgcgc ggggcatccg cggagagggg
     3661 tgcagctgcc ggcgcgggca gcaccatttt gtagcccgca tcggccacgg ctgcggccgc
     3721 ctccgcaagc ccagtcccct caccggagag ggtgaaggtg gccttgccgg tggcgatatt
     3781 aacggcaacc tcactgaggc cgggcaggct cttcagtctt ttttcaaccc gcgccgcaca
     3841 gcctgcgcag gtcatcccct cgatcggaac ctgaatcttt tccatactga tttgacctct
     3901 gctgtaataa aaacgccgca ttctgttatg ataataacaa aatgcggcgg tggatatttc
     3961 gcgcgcgacg cgctctgccg ggccgggcgc ttattttacg gcaaggggcg caatcgacgg
     4021 catggcggcg agaatccgtt ccctctcgcc gagccggtga agaagcgcca ggtattcgag
     4081 ctggctgcgg acctgctcgg gcaggagagg ccaggcgggc agagtggagg cgagggaggc
     4141 cgcctcgagc cggctcctgc tgctgttctg gttgctcagc tgttcgaaaa gctttttgaa
     4201 gagatcccac ggttcctggg gctgcgggaa gacgcgcagg cgcacctttt gatccgcgcc
     4261 gatgccgatg cggcgtttgg ccagatcgag ggccgtgcgc aggccgccga gggtgtcgac
     4321 cagaccatgg gtgtaggcgg cctcaccggt ccagacccgc ccgcgggcga gcagacgcgc
     4381 ctcctcgaag gacttgcccc ggctgtcggc gacgcgctgc acgaagcgct gatagatgtt
     4441 ggcggagagc tggcgaaact tcttcttgtc cttctccgtg aagggataga gtggattgag
     4501 aaagagggcc ccctctgctg tcttgaggga atcggtgtgg gcgccgattt tcttcagggc
     4561 cccgctgaga ttggggatgg tcgagatcac gccgatggag ccggtgatgc tggccgggtg
     4621 ggcgatgagg gtatcgcagg ccatggcgat gtaatatccc cccgaggctg ccacgtcgct
     4681 catcgaggcg tagaccggtt tgattttcct ggcacgcccg atttcttccc agatctcatc
     4741 ggaggccatc accgacccgc cggggctgtc gatgcgcagg atgatggcct tgatacgttt
     4801 gtcgtcgcgc gcctgacgga tatagcgcac gaatttgcgc gaggcgatgg tcgcctcgtc
     4861 gagcaggggg atcatctcgt cttcgcccgg catgatcacg ccggaggcat agatgagggc
     4921 gatctgcttg ccctgaacga cctcctggcc gctcttgtcc gcgctgcggg catagtgggc
     4981 catcgagatc aggggaatct ttttgctgct gtcctgctcg gactcccggg tcaggaggtg
     5041 atcgcggacg ctgccttcgg agcggatggc gtcgatgaag ccgagctcga gcagagagtc
     5101 cgagctgtag aggccgcggt tgaaggcagc cgagaccatg gcgggctcga gcttgcggcc
     5161 ggctgcgact gcctgcagca agatctgctg gcgctggatt agaatctcgc gcagggattg
     5221 gcgggccgga gcactgaagc cggtccggct gtaggactcg cccgccgact tgaactcctc
     5281 gaattgctcg acgtagaact ggacgccgat cttgtccagg gtctccttcc agaagagatc
     5341 ctcgatggca aagccgttga gctcgagcat gccctcggtc ggcatgaaga tggaatcggc
     5401 tgccgaggcg aaaaagtaat ccagttcgcc gccgatatca agataggcct tgaagaactt
     5461 gccggactcc ttgaacttga tcagggcgtc acggatctcc accgccttgg ccatgccgca
     5521 gaggaggtcg ccggagcggt aatagaggcc gcggatgcgg tcatccgtgg cggcccgctg
     5581 gatgccgttg atcagttcga ggaaggtggc gctttgcgtc tccgtgccga gcaggctcag
     5641 gggggcggtc tggagattct caccgagctg gccgcggacg cgcagctcga ccaccgagcc
     5701 gggcttgatc tccaccggtt tttctgaaaa gggccccttg accagggcca ggagtccgac
     5761 catgaaaaag atcacgccga aaacgaaaag gagaaagagg atgagaagga cgatccacca
     5821 gcggcttttc cgccgcgccg cggcgggcat ggtctgctgc tgggtagcgg agtagggggg
     5881 catctgctcc ataaacagct cctggaaatg ggatcgtgaa gagaatcagg atttaccatg
     5941 cgggcgggcg cacgacgccg gccgcatcga cttgttcaac cgaaacgttt tgccaggcgg
     6001 cgatctgcct gatgatgtcg ccgcgcaccc ggtcgcgcag tgcgggcaga tcctccttgg
     6061 acatgccggt ggtctccacc ggcggcagga tctcgatttt gatctcgtgg ggatcgccga
     6121 aaatccacga atgcttgggc atacagtcgc tcgctccctc gatggcgagg gggaggaccg
     6181 gtagctggtc cttgatggcg agcataaaag cgccgtcatt gaaatcgtag acccgccggt
     6241 cctcagaccg ggtcccctcc ggaaagatca tcaccgaaca tttggcttcc aggacgtttc
     6301 tggccgcctg cagggctttg acaccgctgc gcgtgctgga gcggtcgagg gggatatccc
     6361 gcgccatgcg catcatccag cccaagaccg ggatgcgaaa aagcggttcc ttggcgatcc
     6421 atttgcactc catggggatg agggccagca tggggatgtc aagaatggac tggtggttgg
     6481 cgacaacgac gtacggtcgg cgcggattct cgatccggac acccgccatg cgcacgcgcc
     6541 aggatgggcc cagaactgcg atggggtggg ccagccagcg gaagaaacgg ccggtgcggt
     6601 agcgggcggg atcacggtcc agcagccaga tcaacgaggt caagggcagc cagaggacga
     6661 ggagaacgag aaaaatgcac cacaccacgg ccgaaaggag gatgttggta atcttggatc
     6721 ttgtttgcac ctggtgtcgt tccggtttcg ggggaagttt gctataatat acaactgttc
     6781 ggccattgaa aaaagataaa agttggtgct tttaatcaca ttttgcatcc agcaacaagg
     6841 ccaaataagt gttgactttt atccccttgg ataataattt gaaagtaagc cagggggaaa
     6901 gggcaggagt ctgatcaagc gggagtacac ttatggcaca tgatcgagtt ttcttcagac
     6961 tggggatgat cctgtataca ctgacccttg ccagcctggg agcgggagag gcggtcgcac
     7021 aggagctcaa gtgcttcatt ctgacccccc cggagcagat tctggcgggg gtacgccagg
     7081 tagcgatcgc cgatttcacc gtcacctcgc ggcatgaaat ccagcccaaa ccctccggca
     7141 aaaagaacat cgacaaggtc ctcgatacca tcgaaagggt cgcggtcagc ggcaaggagg
     7201 acaaaccgca gcccttcgcc gatgccggca aaaagctcgc cgatctgctc atcgccagca
     7261 tggtcgaagc cgaccgcggc atccgcagtg tcggttcggg attcctcggc atgaagcgca
     7321 aggagggacg cagctttcag gcgggggcgc gcaccaatgt tttcgtcgtg gtggagaggc
     7381 agcgcatgga tcagctgctg gaagagctca aactggggca gagcgggctc gtggatgagt
     7441 cccaggccgc ccaggtcggc aggctgctcg gcgtcgatgc catcgtcacc ggccagatca
     7501 acgccgctgt caaggagagc tgggaggcgg agacccgcac cacgaccaag gggtcgggca
     7561 agaacaagac cgaggagaag aaggaggtct ggtgcaacaa gcgcatcgcc accgtctccg
     7621 caactattcg catcgtcaac gtcgaaaccg gccagctctt cggcac
//
LOCUS       DAOPWU010000159         7666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9551_length_7666_cov_4.070030, whole genome shotgun sequence.
ACCESSION   DAOPWU010000159 DAOPWU010000000
VERSION     DAOPWU010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9551_length_7666_cov_4.070030"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..639)
                     /locus_tag="PKM23_09010"
     CDS             complement(271..639)
                     /locus_tag="PKM23_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91652.1"
                     /translation="MPIPDPKTVAVASERSSDLLVWFEVHPTVSIVAFALACLIVVIA
                     YFAGGRENFRKLWMSILCALIISALAMPIFIKFGRAFGLLKTAGGEIFVLLLVMIFIS
                     ALAAHIYEIITVSAREARPD"
     gene            1258..1458
                     /locus_tag="PKM23_09015"
     CDS             1258..1458
                     /locus_tag="PKM23_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91653.1"
                     /translation="MKKTLFSWTLLLLAGLLCMHCAEKSPQSPAKFNLSKADGCVSCH
                     TNADLLKSLATPLPPVTESGEG"
     gene            1492..2538
                     /locus_tag="PKM23_09020"
     CDS             1492..2538
                     /locus_tag="PKM23_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91654.1"
                     /translation="MVNVHPSFLDSKHGKIGCTGCHGGDATAIEMEKAHAAMVAAPSD
                     GENPVCGTCHGDIAAEHSGSMHKTMKGYYHLIEKRLGRDISTDSELVNHFNKECGKCH
                     ASCGQCHVSRPVSVQGGFVQGHNFYTPPDNKENCTACHGSRIGAEFYGENEGLAADVH
                     WIPNVKRCEFCHDAASIHSTSSGANTRMEDTDIIRCEACHAGAQAGNRWHNKHWGDLS
                     CQVCHSQPYKNCNSCHTGGAGITGSSYMTLKIAKNPLRSPSRPYKYVTVRHIPIARDT
                     YASWGIPDLPNYDSEPTWKYATPHNIKRWTAQTDTSGGAACAEKCHKSSYYLTLEDLP
                     AGEVEANRAYVLTR"
     gene            2609..3607
                     /locus_tag="PKM23_09025"
     CDS             2609..3607
                     /locus_tag="PKM23_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNY91655.1"
                     /translation="MLRKYLMLALIVGLFFAIGCSDDDNNPVDPKKTDEFALVEAAGT
                     AYFSTYVTTSGAAPNITTAALFTLLQDGDATNDPVIVDYRAAADYASGHIRGAINITL
                     ATLVDKLNDNTIPKTKKIVNVCYTGQNSSYATAFMNLLGYDAQSLMFGMCGVSTTAGV
                     NKTDLWINQIAADEFASQLTATVSTPSTVVPFPTLSTGKKSAEEIMKARFPLGISSWS
                     ITAADVFANPSNYFIVNYWPNAEYLNPGHIPGAFCFEPKVSLGKDQMLKYLPTDKTIV
                     IYCYTGQTSAQLACYLQMLGYNAKSLMFGQNGFAYNTLTKSKYTVPTTDYSAIIVK"
     gene            3724..4428
                     /locus_tag="PKM23_09030"
     CDS             3724..4428
                     /locus_tag="PKM23_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91656.1"
                     /translation="MHKLLPVALGLLILFCFSGCSEKSNPLPSKSHPDEWNVASSASF
                     HGKKVLASGNASCTGCHGYELDGSGSAPACQSCHSLYPHDTQWMVLADQEFHGAYIAR
                     DHWSMKSCKKCHGDDYAGGSSKVSCNTCHTGEGGPESCATCHGSLKNPAPPEDLSGNV
                     ATSSMGVGAHQLHMDGYGSCNICHVVPASMSDAGHIDGVEHAEVLAKRSWNRTTGTCV
                     TGCHTSTAKSYIWNSF"
     gene            complement(4538..5542)
                     /locus_tag="PKM23_09035"
     CDS             complement(4538..5542)
                     /locus_tag="PKM23_09035"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNY91657.1"
                     /translation="MAWTKPERLSLANLPTPIDPLVRWSADLGGPSIWVKRDDLTGMD
                     LSGNKIRKLEFVTRAAQDEKADVLITCGAVQSNHARATVIAASRLGMKAHLVLRGVKP
                     ELMQGNYFLDRLYGAECTFITPEEYREQRQQIMDNLAEGYKRLGLRPFVIPEGASDGL
                     GAFGYAHAVAEIMEQEKMLGVRFDAVICAVGSGGTLAGLILGKEIEGWDAQVIGINIC
                     DDADYFTRRVIEILGEVRSLYLPDLIIRPEMISIIDGYIGDGYGKSRPRELQTIIRLA
                     RREALLLDPVYTGKAMHGLVQEIARGRFTGMRNILFLHSGGLFGLMGVTETFQSLLEE
                     "
     gene            complement(5644..6609)
                     /locus_tag="PKM23_09040"
     CDS             complement(5644..6609)
                     /locus_tag="PKM23_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNY91658.1"
                     /translation="MGQKEKAFIGLDLGGTFLKYALGTGDGTILHKGKLPSHGSQSAD
                     AIFNVMFQAVAELLEKAEKEGVEVGAIGVGSPGAIDFNTGRVLGNTPNLPLWTNADIR
                     GRIQEKYHIPVYADNDANIAILAEARFGAAKGCPNVVGLTLGTGIGGGVVINDQLFRG
                     AGFSGAELGHMTINFQGPKCGCGSSGCVEAYASATAMVRNYKEKLAARHMPLPERLNT
                     ELIFAHARSGEEEAMQTIDEVCFYLGVAIGNIINIFNPEIIVIGGGVADAGDAFITRI
                     WQNVDGHVLTAAQSIARLVRAELGNNAGVVGAIALAADSYKAWHM"
     gene            complement(6723..7313)
                     /gene="def"
                     /locus_tag="PKM23_09045"
     CDS             complement(6723..7313)
                     /gene="def"
                     /locus_tag="PKM23_09045"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNY91659.1"
                     /translation="MAILPVIKYGHPTLRKKAKAVHPAELAESFIRDMVETMEALDGV
                     GLAANQVNVPKQLLVAKDVDHKKLHILINPEIVAYSEKKESDIEGCLSLPGLQAEVPR
                     YFKIIVKAQTPAGEEIEITARGHFARVLQHEIDHLNGILYIDRANADTLVWIIEDGRK
                     EYSTKNATLPDIQHQYTSRYHTGLQGIRFEAETEIA"
     gene            complement(7324..>7666)
                     /locus_tag="PKM23_09050"
     CDS             complement(7324..>7666)
                     /locus_tag="PKM23_09050"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903467.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNY91660.1"
                     /translation="VRPQADYHRSLDLLDSARRHGARTKSGLMLGVGESRDQLIKTLR
                     DLRNIDCQIITLGQYLPPSRHHLPVERFVEPTEFAELRRLGLEMGFPHVESGPLVRSS
                     YHAEEAADSAN"
BASE COUNT         1693 a         2254 c         2089 g         1630 t
ORIGIN      
        1 aggcaaagcc ggcgatagcc agcagcactg cagcagctgc tatcatcagg gtcttgccat
       61 gtacttcaag ccactcctgg gccttgaagt aaaaggtgac cagggagtct tccttgagct
      121 gcttcttggt aatctgtttt ctcggtttta gcatctgccg tccgaatgaa taggttaagg
      181 tagtctattg ttgattaaaa cttgttaata taaggaggaa ttcgcggaaa tgcaacagaa
      241 aacctagaaa agggggattt gcaggccagg ttaatccgga cgcgcctcgc gggccgagac
      301 ggtgatgatc tcatagatgt gggcggcgag ggctgaaatg aagatcatca ccagcagcag
      361 gacaaagatt tcgcctccgg ccgtcttgag cagtccgaaa gcgcgcccga acttgataaa
      421 gatcggcatg gccagcgcgc tgatgatcag ggcgcaaagg atgctcatcc agagcttgcg
      481 gaaattctca cgaccgccgg cgaaataagc gatgaccacg atcaaacagg ccagggcaaa
      541 ggcgacaatg gaaacggtgg gatggacttc aaaccagacc agcagatcgc tgctgcgctc
      601 actggccact gcaactgttt tgggatcagg aataggcata atcgccccgc actaaataat
      661 ttgcacccaa ccgaggggcc tgcgcggcca tcctctgctt aattttgtaa aaatatggag
      721 aaatagcaag ctatttctgc cgggcttgcc aggattgact gctcccgtcg accatttccc
      781 ggtgcaggcc atcagacgcc ctccatcccg taaacaggtc ctttgccacg atcgctcctt
      841 cgactgcaga aaaatacgac aattattgtg atttcagcgc ctgcattgtt gtttttttta
      901 tattttttgc ttaagctatt tagtttaaaa caaaattcac ggtatatgcc cgtgcccggg
      961 cgttcttttc cgggacggtc aaaaagggct ggccgcttgt ttcacttgaa attctgtttt
     1021 taatctttta gtataatttg cccgctatgg gcccctctct tgcactttag tggacagttc
     1081 tttctcaccg gtcgcgacga tactgctgca gcaacacagg cagccgataa gaccggattt
     1141 gatttccgat atatcctgca ggcgtgtgtc cgcccgaagg tacaacaccg acctgtggag
     1201 acgagctgca cccttaatca acctgcccac agttcaacga ccgatggaga ctaatcagtg
     1261 aaaaagaccc tattctcctg gacactgctt ctcctcgccg gactcttgtg catgcactgc
     1321 gctgaaaaga gtccacaatc cccggccaaa tttaatttga gcaaggcgga tggatgcgtc
     1381 tcctgccata ccaacgccga tcttctcaaa tccctcgcca cccccctccc tcctgtcacc
     1441 gaatcgggcg aaggctgagg cggctctgtg tctcaggttg aggcatggca gatggttaat
     1501 gttcatcctt cgttcctcga ttcaaagcac ggcaaaatcg gctgcaccgg ctgtcatggt
     1561 ggcgatgcta ctgccatcga aatggaaaag gcccatgccg ccatggtcgc cgcccccagc
     1621 gacggagaga atccggtttg cggaacctgt catggtgaca ttgcggccga gcacagcggc
     1681 agtatgcaca agacaatgaa ggggtattac catctcatcg aaaagcgtct gggccgggac
     1741 atctccaccg acagcgagct ggtgaaccat ttcaacaagg agtgcggcaa gtgccacgcc
     1801 agctgcggac agtgtcatgt ctccaggccg gtctcggttc aaggcggctt cgttcaaggc
     1861 cacaatttct acactccgcc cgacaacaag gaaaactgca cggcctgtca cggcagccgc
     1921 atcggcgccg agttctatgg ggaaaatgag gggctggctg ctgacgtgca ctggattccc
     1981 aatgtcaagc gctgcgagtt ctgtcacgat gccgcctcca tccactccac ttccagcgga
     2041 gcgaacacgc gcatggaaga caccgacatc atccgctgtg aggcatgcca cgccggtgcg
     2101 caggccggaa accgctggca taacaagcac tggggtgatc tctcatgtca ggtctgtcac
     2161 tcccagccct acaagaactg caacagctgt cacaccggcg gggcgggcat taccggcagt
     2221 tcctatatga ccctgaaaat cgccaagaat cccctgcgct ccccctcccg gccttacaag
     2281 tatgtcaccg tgcgccacat cccgatcgcg cgggacacgt acgcgagttg ggggatcccg
     2341 gatctgccca actatgattc tgagccgacc tggaaatatg ccactccgca caatatcaag
     2401 cgctggacgg cccagactga caccagcggc ggcgccgcct gcgcagaaaa gtgccacaag
     2461 agcagctact atctgactct cgaggatctt cctgccggag aggtcgaggc caaccgggct
     2521 tatgtattaa ctcgttagaa ccatggcaaa agaagataaa caaccctaat ccatcacacc
     2581 tgataacaac tcaccaagga gaaatcgcat gttgcgcaag tacctgatgc tggctttgat
     2641 tgtaggactg ttttttgcta tcggctgcag tgacgatgac aacaaccctg ttgaccccaa
     2701 gaagacggac gaattcgccc tcgtcgaggc cgccggcact gcctatttca gcacctacgt
     2761 taccacctcc ggggccgccc ccaatattac caccgcggcc ctcttcaccc ttttgcagga
     2821 cggggatgcc actaatgatc cggtcatcgt cgattaccgt gctgcagccg actatgcttc
     2881 cggccatatc cgcggcgcca tcaatatcac cctggccaca ctggtcgaca agctcaacga
     2941 caacaccatc cccaagacca aaaaaatcgt caatgtctgc tacaccggcc agaactcctc
     3001 ctatgccacg gcctttatga acctgctcgg ctacgatgcc caaagcctga tgttcggcat
     3061 gtgcggcgtc agcaccaccg ccggcgtcaa caagaccgat ctctggatca accagatcgc
     3121 agctgatgag ttcgcctccc agctgaccgc gacggtgagc accccctcga cggtggtccc
     3181 cttcccgacc ctctcgaccg gcaagaagag cgccgaggag atcatgaagg cgcgtttccc
     3241 gctcgggatc agcagctggt cgatcaccgc tgccgatgtc ttcgccaatc cgtccaacta
     3301 tttcatcgtc aactactggc cgaacgccga atacctcaat cccggccaca tccccggtgc
     3361 tttctgcttc gagccgaagg tctctctggg caaggatcag atgctgaagt acctgccgac
     3421 ggacaagacc atcgtcatct actgctacac cggacagact tccgcccaac tcgcctgcta
     3481 cctgcagatg ctgggctaca atgccaagag tctgatgttc ggccagaacg gtttcgccta
     3541 caacaccctg accaagagca agtacaccgt tccgaccacc gattattccg cgatcatcgt
     3601 aaagtaatcc ctgacggggg tgcgcctgcc ggcgcgcccc gtcaattccc gaggctccac
     3661 ctgcagggtg aactcgaaaa agccgtatcc gcggggcata atcgcatcca gaaagggcga
     3721 tccatgcata aactccttcc cgtcgcgctg ggtttgttga tcctgttctg cttctccggc
     3781 tgttcagaaa agagcaatcc tctgccgtca aaaagccatc cggatgaatg gaatgttgcc
     3841 tcctccgcaa gctttcacgg taaaaaagtg ctcgcaagcg gcaatgcctc ctgcacagga
     3901 tgtcatggct atgaactcga tggcagcggc tccgctccgg cctgccagag ctgtcactct
     3961 ctttatcccc atgataccca gtggatggtc ctcgctgacc aggaatttca tggggcctat
     4021 attgccagag accactggtc gatgaagagc tgcaaaaaat gtcatggtga tgattatgcc
     4081 ggcggcagct caaaggtatc ctgcaacacc tgccacaccg gcgagggtgg cccggaatcc
     4141 tgcgccacct gtcacggcag cctcaagaat cctgcccctc ccgaagacct ttcgggtaat
     4201 gtcgccacca gctcgatggg tgtcggcgcc caccagctcc acatggatgg atatggaagc
     4261 tgcaacatct gccatgtcgt tccggccagt atgagcgatg ccggccacat cgatggcgtc
     4321 gaacacgccg aggtgctggc gaagagaagc tggaaccgca ccaccggcac ctgcgtcacc
     4381 ggctgccaca ccagcacggc aaaaagctac atctggaaca gcttttgata tcctgctgaa
     4441 cgcctgacca caacgtacaa aaaagccctc acactcagca cagctgagcg gagggctttt
     4501 ttacttaaag gcggaagcca gcaggatgct ctggcgtcta ctcctccagc agggattgga
     4561 aggtctccgt cacccccatc agtccaaaga gaccgccgct gtggagaaag aggatgttgc
     4621 gcatcccggt gaagcggccc ctggcgattt cctgcaccag tccgtgcata gccttgcctg
     4681 tataaaccgg atcgaggagc agggcctcgc gtctggccag gcggatgatg gtctgcagtt
     4741 cccggggcct ggatttaccg tagccgtcac cgatataacc gtcgatgatg ctgatcatct
     4801 ccggccggat gatcaggtca ggcaggtaaa gcgagcgtac ctcaccaaga atctcgatga
     4861 cgcggcgtgt gaaatagtcg gcgtcatcgc agatgttaat gccgatgacc tgtgcatccc
     4921 acccctcgat ctccttgcca aggatcaggc cggccagggt accacccgag cccacggcgc
     4981 atattacagc atcgaaacgg acgcccagca ttttctcctg ttccatgatc tcggcgacgg
     5041 catgggcgta tccaaaagcg ccgaggccgt ccgaggcccc ttccgggatg acgaaaggac
     5101 gcagtcccag gcgtttatag ccctccgcga ggttgtccat gatctgctgt ctttgctccc
     5161 ggtactcctc aggggtgata aaggtacatt cggccccgta gaggcggtca aggaaatagt
     5221 taccctgcat gagctcaggc ttgacgccgc gcaggacgag atgggctttc atgcccagac
     5281 gggaagcggc gatgaccgta gcgcgggcgt ggttggactg aactgcaccg caggtgatga
     5341 gcacatccgc tttctcgtcc tgcgccgccc gggtcacgaa ttccagcttc ctgatcttgt
     5401 tgccgctcag gtccataccg gtcagatcgt cccgcttgac ccagatggat ggtcctccga
     5461 gatcagcgct ccagcgcaca agcggatcaa tcggggtggg cagattggcc agggacagac
     5521 gttcgggttt ggtccaagcc atggaacttc tcctggagaa ggggtaaggt gaacatcgcc
     5581 gcacgagttt aattctgtta aaagaaaaag cctcccggca ggctcaccgg aaggcttggc
     5641 atctcacatg tgccaggctt tatagctgtc ggcggcgagg gcgatcgccc cgaccacgcc
     5701 ggcattgttg cccagctccg ccctcaccag gcgggcgatg ctttgtgctg cagtgaggac
     5761 gtggccgtca acattttgcc atatgcgggt gatgaaagca tccccggcgt cggcgacacc
     5821 gccgccgata acgataattt ccgggttgaa gatattgatg atattgccaa tggccacccc
     5881 cagataaaag caaacttcat cgattgtctg catggcctct tcttcaccac tgcgggcgtg
     5941 ggcgaagatc agctcggtat tgagccgttc gggcagtggc atgtggcggg cggcaagctt
     6001 ttccttatag ttcctgacca tagcagtggc cgaggcatag gcctcgacac aacctgagct
     6061 gccgcagccg catttgggac cctgaaaatt aatggtcata tggcccagtt cagcaccgga
     6121 aaagccggct ccgcggaaaa gctgatcgtt gatgaccacg ccgcccccga tacccgttcc
     6181 aagggtaagg ccgacgacgt tggggcatcc cttggcggcg ccgaagcggg cttcggcgag
     6241 aatggcaata ttggcatcat tgtccgcata gacggggatg tggtatttct cctggatccg
     6301 gccgcggata tcggcattgg tccataaggg cagattgggc gtattgccga gtacccgccc
     6361 ggtgttgaaa tcgatcgcgc ccgggctgcc gaccccgatg gcgcccacct ccacgccctc
     6421 cttctcagcc ttttcgagca gttcggcgac ggcctggaac attacgttga aaatcgcgtc
     6481 agccgactgg ctgccatggc ttggcagctt gcccttgtgc agaatggttc cgtcccccgt
     6541 gccgagggca tattttaaaa aagtgcctcc cagatcaaga ccgatgaatg ccttttcctt
     6601 ctgccccatt ctatctcctg tggattggtc gtgttgtaac gaacaagtgg tctgttccgc
     6661 ttcccgagcc gtcccttcaa atggcagcgt gtccggcaac atacaaaata aagcggagat
     6721 tatcaagcaa tttcggtttc ggcctcgaaa cggattccct gcagtccggt gtgataccgg
     6781 ctggtgtatt ggtgctggat atcaggcagg gtggcgtttt tggtcgaata ctctttgcgg
     6841 ccatcctcga tgatccagac cagggtatcc gcattggcgc ggtcgatgta gaggatgccg
     6901 ttaaggtggt cgatctcgtg ctgaagaacg cgggcaaagt ggcccctggc tgtgatctcg
     6961 atctcctcac ctgcgggggt ttgggccttg acgatgatct tgaaatatcg cggtacttcc
     7021 gcctgcagac cgggcaagct gagacagccc tcgatatcgc tctccttctt ttcgctgtag
     7081 gccacgatct cgggattgat gagaatgtgg agctttttgt ggtcgacgtc tttggcgacg
     7141 agaagttgtt tggggacatt gacctggttc gcagcgaggc cgacgccatc gagggcctcc
     7201 atggtttcaa ccatgtcccg aatgaaggat tccgccagtt cagcggggtg caccgctttc
     7261 gcctttttgc gcagggtggg atgtccgtat ttgatgacgg ggagaatagc catgatatta
     7321 ccttcagtta gcagagtccg ccgcctcttc ggcgtggtaa gagctgcgca ccagcggtcc
     7381 ggactcgaca tgggggaaac ccatttccag cccgaggcgg cgcagttcag caaattcagt
     7441 aggttctaca aatcgttcaa ccggcaggtg atggcgcgag gggggcaaat attgcccgag
     7501 ggtgatgatc tgacagtcga tgttgcgcag atccctgagg gttttaatca gctggtcgcg
     7561 gctctctccc acgcccagca tcagccccga tttagtcctg gcgccgtgac ggcgtgcaga
     7621 gtcgaggagg tcgagggagc ggtggtaatc ggcctgtggt ctgacc
//
LOCUS       DAOPWU010000160         7643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9591_length_7643_cov_5.093832, whole genome shotgun sequence.
ACCESSION   DAOPWU010000160 DAOPWU010000000
VERSION     DAOPWU010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9591_length_7643_cov_5.093832"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PKM23_09055"
     CDS             complement(<1..1024)
                     /locus_tag="PKM23_09055"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNY91661.1"
                     /translation="MSVTIILGAQWGDEGKGKIVDLLSAKADVVARYQGGPNAGHTVV
                     IDEEEIVLHQIPSGILHPHTRCIIGNGVVIDPVILMEEVDFVKSKGFQVDGRLFISHR
                     AHLIMPYHKLLDTTREAAEGASKIGTTGRGIGPAYVDKFDRCGIRIVDLLDRSTLAEK
                     LLKNLESKNKVLQRIYDRDSMDTEKIIQDYLEFDRRIDAYVKDTTLLINRAIDASEEV
                     LIEGAQGTMLDIDFGTYPYVTSSNPTAGGACTGLGVGPTRIDRVLGVVKAYTTRVGMG
                     PMPTEFDTEFGDQMRQLGGEYGATTGRPRRCGWFDAVVVRMAAMINGLTHLAITKLDV
                     LDTLPEI"
     gene            complement(1100..1447)
                     /locus_tag="PKM23_09060"
     CDS             complement(1100..1447)
                     /locus_tag="PKM23_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY91662.1"
                     /translation="MEVTERKEGAVAILTLQGPYVGAAEAGVIMDTLHELRERGEKLI
                     VIDLSRVQTMNSSGLGALISGLNTMRQAGGDLKLAGVNEKVDKLLAITKLHSVFESFA
                     SVEEAKASFAGLA"
     gene            complement(1478..2407)
                     /gene="secF"
                     /locus_tag="PKM23_09065"
     CDS             complement(1478..2407)
                     /gene="secF"
                     /locus_tag="PKM23_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNY91663.1"
                     /translation="MEFFKKTNFDFIGMRKYAYAFSIITFVISLVYLFIHGPNYGIDF
                     TGGTSLQIKFNTPTEPSQIRSAISGAGYGDAEIKNLGDTANNSFIVRIPQMGEEAKAG
                     DIVLQELKTNLADHPYEILSVTQIGPKIGGELRRGAYYSILVALIGMLIYISWRFEFK
                     FAVTAVISIFHDVIFVLGIYTILGLEMSLTALAAFLTIVGYSINDTIVVYDRIRENLK
                     LMRRESMATVMNTSINQTLSRTLLTGVSTLVVLLVLIFMGGEVIHGFSLALFFGTLIG
                     TYSSVFVASTLVLDWNLYLERKKALKTAARAAR"
     gene            complement(2448..4487)
                     /gene="secD"
                     /locus_tag="PKM23_09070"
     CDS             complement(2448..4487)
                     /gene="secD"
                     /locus_tag="PKM23_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNY91664.1"
                     /translation="MQRALIFKLIFVLALIVVAVLSMLPTFQVNNLLDQESALISKLG
                     NLTGLSKTDIEVGVTSGELEGHVRRAVSESSRNEALRLSDDLIKLNSRIAKLQGRAIR
                     RGLDLQGGTYLVYEIDLKQYLAENAKNKDNRLEDILAASQRDYENKGTDFFDALQENF
                     ASRDLKLNRYFGRRGQSDDEIITDLQKLSKDAVDRTLQVLRNRVDQFGVSEPSITPQG
                     NSRILIELPGIQNIERAKKVIGTTALLEFKLVKDPEIVWSVLNDIDKALRAKRQGVDV
                     AAVKSDTAVTTGDKAAEKQLSLNELFGDQTTSESGEDTSIVVDQATFDEKPFTSLLRG
                     LPGRYSDVVSVPAQNIRAIQRALQLPEVQAVIPSDVQLLWGTREIAMGDQAFQRLYVV
                     KKEAELLGSMLSNADVQIDGGSQSLSAGQAEVHMELNPEGTKTFAKVTGMNVGKQLAI
                     VLDGRVESAPNIKEKIPSGSARIDGMGSMEEAQDLALVLRAGALPAPVKVLQEDTVGP
                     SLGQDSVSKGARSALIGILLVMIFVVIYYNLVGLVADFALILNALFVMAIMAGFGFTL
                     TLPGIAGFILSVAMAIDSNVLINERIREELRGGKTTRAAIEQGYGRALITILDSNITT
                     LITGAVLYTFGTGSVKGFAVTLCIGILVSMFTAIYVTRLILDLATIRFGVKKLSI"
     assembly_gap    4601..4700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4701..5249)
                     /locus_tag="PKM23_09075"
     CDS             complement(<4701..5249)
                     /locus_tag="PKM23_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91665.1"
                     /translation="MTRTFMEVVLKTDARFAKGFLQGYLAGSGKAHRFFINEEAGIEA
                     ESLSEKLKELVGLADLFQRVVIEEDFLALLHKDSAAAAALGLSGQPLQARRITSGSFS
                     VRIKDSAQEDAAAIKKLLSGRPAGVTVEGWQEHETIEKNGRGVELYTPLHDYVYEASG
                     SFRGPIEALADLRSELGQHASVK"
     gene            complement(5295..6845)
                     /gene="citF"
                     /locus_tag="PKM23_09080"
     CDS             complement(5295..6845)
                     /gene="citF"
                     /locus_tag="PKM23_09080"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HNY91666.1"
                     /translation="MSMVKNSLGRLVPTEVNGRSVRPFMGAHADAGGGMRYGGSIPAA
                     ARYGNKLLADLDAAIDACEIRDGMVISFHHHLRNGDYLVNLVMDKLAARGLKDLVLAP
                     SALFPTHGRLAEYIESEVISHIEGSMNGPIGRACSLGKMKKSAILRSHGGRYRAIQDG
                     DLHIDVAFIAAPCADPHGNANGVNGPSACGPLGFALADSLFADKVVVVTDHLVDFPCH
                     PWSIEGGNVDFVVRIDSIGDPNQIVSGTTQITRSPTRLLIAEYAARFVKESGIMTDPH
                     FSFQAGAGGVSLAFVKYMSDLMREAGVAADFVRGGSTKYMVGMLENGLTRYILDGQSF
                     DLDGVRSLRDNPCHIETNPFVSYDYHTKGCFAQLLKVSVLGATEIDLDYNVNVNTHSD
                     GWLLHGIGGFTDAADAEVTMITAPLVRSRLPIVLDSVTTVTAPGEMIDVVVTEHGIAI
                     NPRRQDLLDRLKGSTLPLKTIEELRDIAARMSGVPEKPQFADRVVAVIEFRDGTIIDA
                     VRELVPPE"
     gene            complement(6842..>7643)
                     /locus_tag="PKM23_09085"
     CDS             complement(6842..>7643)
                     /locus_tag="PKM23_09085"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNY91667.1"
                     /translation="ILDLEDSVAPPEKFVTRILVKHALAALEFGSSERIVRINPLQTP
                     YGAEDLEEIVAAAPDTLLIPKCESAADVRAVEEQVAALEAKAGLAQPLLFMPLIETAR
                     GVLHADEIATASSRNIALCFGAEDFTADLGVERTVEGREHFVARSLIVLAAKAAGIQA
                     IDTVYSDVQDVEGLSASAREAMAQGFDGKGVIHPSQIKSVHAAFCPSPEQIEKAQKIV
                     AALEEAKAKGSGVVSLGTKMIDAPVVARAQKTLERARAHGVLGEEEKI"
BASE COUNT         1474 a         2224 c         2374 g         1471 t
ORIGIN      
        1 tgatctcggg gagggtgtcg aggacgtcaa gtttggtaat ggccagatgg gtgaggccgt
       61 tgatcatcgc cgccatgcgc acgaccacgg catcgaacca gccgcagcgc cgcggacggc
      121 cggtggtggc gccgtattcg ccgcccagct ggcgcatctg gtcaccgaac tcggtatcga
      181 actcggtcgg catcggcccc atgccgacgc gggtggtgta ggccttgacc accccgagca
      241 ccctgtcgat ccgggtcggg ccgacgccca ggccggtgca ggcccccccg gcagtgggat
      301 tggaagaggt gacataggga taggttccga aatcgatgtc gagcatggtc ccctgcgccc
      361 cctcgatcag aacctcctca ctcgcgtcga tggcgcggtt gataagcagg gtggtatcct
      421 tgacgtaagc atcgatgcgg cggtcgaatt cgagatagtc ctgaatgatt ttttcggtat
      481 ccatgctgtc gcggtcgtag atgcgctgca gcaccttgtt cttgctctcg aggtttttaa
      541 gcagcttctc ggccagggtg gagcgatcga gcagatcgac aatgcggatg ccgcagcggt
      601 cgaatttgtc gacgtaggcc ggtccgatcc cgcgcccggt agtgccgatt ttggacgccc
      661 cctcggccgc ctcacgggtg gtatcgagca gcttgtggta gggcataatc aaatgggcgc
      721 ggtggctgat gaaaaggcgt ccgtcgacct ggaagccctt gctcttgacg aaatcgacct
      781 cctccatcag aatgaccgga tcgatgacca ccccgttgcc gatgatgcag cgggtgtgcg
      841 gatgaaggat gccggaggga atctgatgga ggacgatctc ctcctcatcg atgacaactg
      901 tatgacccgc gttagggccg ccctgataac gcgcgaccac atccgctttt gcgctgagca
      961 gatcgacgat cttgcctttg ccttcgtcgc cccactgggc gccaaggatg atggtgacgg
     1021 acataggttc ctctacaagt aaaaaactcc ggaccgtggc cggtccggag tttgcactgc
     1081 actgcccttt aaatcgggat caggccaaac cggcaaagct cgccttcgcc tcctcgacgg
     1141 aggcgaagct ctcaaagact gaatgcagtt tggtgatggc cagcagcttg tccacctttt
     1201 cattcactcc tgcaagcttg agatcccctc cggcctgacg catggtgttc agtccgctga
     1261 tcagggcgcc cagacccgag ctgttcatgg tctgcacccg tgaaagatcg atgacgatca
     1321 acttctcccc ccgctcgcgc agttcatgga gggtatccat gatcacgccc gcctcagcgg
     1381 cgccgacata tgggccctgc agggtcagga tggcgaccgc gccctctttc cgttctgtca
     1441 cttccatagg ggtctcctcg cttgccgcgg ctttccgcta gcgggcggcg cgcgccgccg
     1501 tcttcagggc cttcttccgc tccagataaa gattccagtc gagcaccagg gtactggcga
     1561 cgaagaccga tgagtaggtg ccgattaggg tgccgaagaa gagggccagg gagaagccgt
     1621 ggatcacctc gccgcccata aagatcagca ccagcagcac gaccagggtc gagacgccgg
     1681 tgagcagggt gcggctcaag gtctggttga tgcttgtgtt catcaccgtg gccatggatt
     1741 cgcggcgcat cagcttgaga ttctcgcgga tgcggtcata gaccacgatg gtgtcattga
     1801 tcgaataacc gacgatggtg agaaaggcgg ccagggcggt cagcgacatc tccagcccga
     1861 gaatggtata gatgccgagc acaaagatga cgtcatgaaa gatcgagatc accgccgtca
     1921 cggcgaactt gaactcaaag cgccagctga tgtagatgag catgccgatg agggcgacca
     1981 ggatcgagta gtaggcgccc cggcgcagtt caccgccgat cttggggccg atctgggtga
     2041 cgctgagaat ctcataggga tgatcggcca ggttggtctt gagctcctgc aggacgatat
     2101 cgcccgcctt ggcctcctcc cccatttgcg gaatacggac gatgaaggag ttgttggccg
     2161 tgtcgccgag attcttgatc tcggcatcgc catagccggc tccgctgatg gcgctgcgga
     2221 tctggctcgg ctcggtgggg gtgttgaatt ttatctgcag tgaggtgccg ccggtgaaat
     2281 cgatgccgta gttggggccg tggatgaaca gataaaccag gctgatgacg aaggttatga
     2341 tcgaaaaggc ataggcgtac ttgcgcatgc cgatgaaatc aaaattggtt ttcttgaaaa
     2401 attccatact cgccaaatct ccttgaggaa aggccttgcc ggcccgatta aatgctcagt
     2461 ttcttcacgc cgaaacggat agtcgcgaga tcgaggatca gccgcgtcac gtagatcgca
     2521 gtgaacatac tcacgaggat accgatgcag agggtcaccg caaaaccctt cacggagccg
     2581 gtgccaaagg tgtaaagcac cgcgccggtg atcagggtgg ttatgttgga atcgagaatg
     2641 gtgatcaacg cgcggccgta cccctgttcg atcgccgcgc gcgtggtctt gcctccgcgc
     2701 agctcttcac ggatacgctc attgatgagc acattggaat cgatggccat ggccacagag
     2761 aggatgaaac cggcgatgcc cggcagggtg agggtaaagc cgaagccggc catgatcgcc
     2821 atgacgaaga gggcgttgag aataagcgcg aaatcagcga ccaggccgac cagattgtaa
     2881 tagatgacca caaatatcat caccagcagg atgccgataa gggcggacct ggcccccttg
     2941 ctgaccgaat cctggcccag cgaaggacca acagtatctt cctggagcac cttcaccgga
     3001 gccggaaggg cgccggcgcg aaggaccagg gccagatcct gcgcctcctc catgctgccc
     3061 atgccgtcga tgcgggcgct gccggaggga attttttcct tgatgttcgg ggccgattcg
     3121 acgcggccgt cgagcacaat cgccagctgc ttgccgacat tcatgccggt caccttggca
     3181 aaggtcttgg tgccttcagg attgagctcc atgtggacct ccgcctgacc ggcgctcagg
     3241 ctctgcgagc cgccgtcgat ctggacatcc gcattgctca gcatcgagcc gagcagctcg
     3301 gcctctttct tgacgacata gagacgctgg aaagcctggt cgcccatggc gatctcgcgg
     3361 gtgccccaga gcagctggac atcgctgggg atgaccgctt gcacctcggg gagctgcagg
     3421 gcgcgctgga tcgcacggat gttctgcgcg gggacactga ccacatcgga atagcggccg
     3481 ggcagcccac gcagaagaga ggtgaaaggc ttttcatcaa aggtcgcctg atccaccacg
     3541 atcgaggtat cctcgccgga ctcgctggtg gtctggtcgc cgaaaagttc gttgagactg
     3601 agctgcttct ctgcagcctt gtcgccggtg gtgacggcgg tgtcgctctt gaccgcggcc
     3661 acgtcaacac cctgacgctt ggcgcgcagc gccttgtcga tatcattgag cactgaccag
     3721 acgatctcgg gatccttcac cagcttgaac tcgagcagtg ccgtggtgcc gatgaccttc
     3781 ttggcgcgct cgatgttctg gatgcccggc agctcgatga ggatacggct gttgccctgc
     3841 ggggtgatgc tgggctcgga gacgccgaac tggtcgacgc gattgcgcag cacctgcagg
     3901 gtgcggtcga ccgcatcctt ggagagcttt tgcaggtcgg tgatgatctc gtcatcggac
     3961 tggccgcggc ggccgaaata acgattgagc ttgagatccc gggaggcgaa attctcctgc
     4021 agggcgtcaa aaaagtcggt gcccttgttt tcataatcgc gctgcgaagc ggcgagaatg
     4081 tcctcgagcc gattgtcctt gttcttggcg ttctccgcca ggtattgctt caggtcgatc
     4141 tcgtagacca gataggtgcc gccctggagg tcgaggccgc ggcggatcgc acggccctgc
     4201 agcttggcaa ttctgctgtt aagcttgatc agatcgtcgg agaggcgcag ggcttcattc
     4261 ctgctgctct cggagaccgc ccggcgcaca tggccctcga gctcgccgct ggtgacgccg
     4321 acctcaatat cggtcttgct caacccggtc agattaccga gcttggagat cagcgcggac
     4381 tcctggtcca gcaggttatt gacctgaaag gtgggcaaca tggagaggac agccacgacg
     4441 atcagggcca acacgaaaat aagcttgaaa atcagagcac gttgcatgaa tggaaaacct
     4501 cctgaaaggg attgattttg cggatataac caagaaatat accctttttt gccaaaatat
     4561 gcaaacaaaa agaacgagcc cctcatcctg aggggctctg nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn cttgacggaa gcatgctgac ccagctccga gcgcaaatcg
     4741 gcaagggctt cgatcggacc gcgaaaggag ccggaggctt cgtagacata atcatgcagg
     4801 ggggtataaa gctccacccc gcggccgttc ttttcgatgg tctcgtgctc ctgccacccc
     4861 tccaccgtca cgccggcggg cctcccgctg aggagcttct tgatggctgc ggcgtcctcc
     4921 tgcgcagagt ccttgatccg gacggaaaag gagccggagg tgatccttct ggcctgaagg
     4981 ggctggccgg aaaggccaag ggcggccgct gccgccgaat ccttgtgcag cagcgccaga
     5041 aaatcctcct cgatgacgac gcgctggaaa agatcggcga ggccgaccag ttccttcagc
     5101 ttttcggaga gcgattcggc ttcgatcccg gcctcttcat tgatgaaaaa acggtgggcc
     5161 ttgccgctgc cggcgagata gccctggagg aaacccttgg caaaacgggc atccgtctta
     5221 agcaccactt ccataaacgt cctggtcata tccttctcct gaaatcagat ttccactctt
     5281 ctgacggcgc cgcatcactc gggcggcacc agttcgcgca cggcatcgat gatggtgccg
     5341 tcgcggaact cgatcaccgc caccacccgg tcggcgaact ggggcttttc gggcacaccc
     5401 gacatgcggg cggcgatgtc gcgcagctct tcgatcgtct tcaggggcag ggtgctgccc
     5461 ttgagccggt caagcagatc ctgccggcgg ggattgatgg cgatgccgtg ttcggtcacc
     5521 accacgtcga tcatctcgcc cggggcggtc acggtggtga cgctgtcgag gacgatcggc
     5581 agccggctgc gcaccagggg ggcggtgatc atcgtcacct cggcgtcggc cgcatcggta
     5641 aaaccgccga tgccatgcag cagccagcca tccgagtggg tattgacgtt gacattgtaa
     5701 tcgaggtcga tctcggtcgc ccccagcacc gaaaccttga gtagctgggc gaagcagccc
     5761 ttggtgtgat agtcatacga gacaaaagga ttggtctcaa tatggcaggg gttgtcgcgc
     5821 agggagcgca ccccgtccag atcgaagctc tggccgtcca gaatatagcg ggtcaagccg
     5881 ttctcgagca tcccgaccat atatttggtc gaaccgccgc ggacgaaatc ggccgccacc
     5941 cccgcctcgc gcatgagatc gctcatatac ttgacaaagg cgagcgagac cccgccggcc
     6001 ccggcctgaa aactgaaatg gggatcggtc atgatgccgg actctttgac gaagcgggcg
     6061 gcatattcgg cgatgagcaa gcgggtgggc gagcgggtga tctgggtcgt gccggagacg
     6121 atctgattcg ggtcgccaat ggaatcgatc cggaccacaa aatcgacatt gcccccctcg
     6181 atgctccagg gatggcaggg aaaatcgacg agatgatcgg tcaccaccac caccttgtcg
     6241 gcgaagaggg agtcggcgag ggcgaagccg agcggaccgc aggctgaggg gccgttgact
     6301 ccgttggcgt tgccatgggg atcggcgcag ggagcggcga tgaaagcgac atcgatatgg
     6361 agatcgccgt cctggatggc gcggtagcgg ccgccgtgcg agcgcaggat cgcgctcttt
     6421 ttcatcttgc ccagggagca ggcgcggccg atggggccgt tcatcgatcc ctcgatgtga
     6481 gagatgacct cggactcgat atactcggcc agcctgccgt gggtcggaaa gagggccgag
     6541 ggggcgagga ccagatcctt gaggccgcgc gcggccagct tgtccatcac caggttgacc
     6601 agatagtcgc cgttgcgcag atgatgatga aaggagatga ccatgccgtc gcggatctcg
     6661 caggcgtcga tcgctgcgtc gagatcggcc agcagtttgt tcccgtagcg ggcggcggcg
     6721 gggatcgatc cgccgtagcg catgcccccg ccggcatcgg catgagcccc catgaaaggc
     6781 cgcacgctgc ggccgttgac ctctgtgggc accaggcgcc cgagactgtt cttcaccatg
     6841 ctcatatctt ttcttcctct ccgagaacgc catgggcgcg ggcgcgctcg agagtctttt
     6901 gtgcgcgcgc caccactggg gcatcgatca tcttggtgcc gagagagacc acccccgatc
     6961 ccttggcctt ggcctcctcc agggcggcca caattttctg cgctttttcg atttgctccg
     7021 gcgagggaca aaaggccgca tgcaccgatt tgatctggct gggatggatc acccccttgc
     7081 cgtcaaagcc ctgggccatc gcctcgcggg cgcttgcgct cagcccttcc acatcctgta
     7141 catcggagta gaccgtatcg atcgcctgga tcccggccgc cttggccgcc agcacgatca
     7201 ggctgcgggc cacaaagtgt tcccggccct cgaccgtgcg ctccaccccc agatcggcgg
     7261 tgaaatcctc ggccccgaag cagagggcga tattgcggct gctggcggtg gcgatctcgt
     7321 cggcgtgaag cacgccccgg gcggtctcga tcaggggcat gaagaggagg ggttgggcga
     7381 gtcccgcctt cgcctccagg gccgccacct gctcctcgac ggcgcgtacg tcggccgcgc
     7441 tctcgcactt ggggatgagc aaggtgtcgg gggcggcggc aacaatctct tcgagatcct
     7501 ctgcgccgta gggggtctgc aggggattga tgcgcacaat gcgctcgctg ctgccgaatt
     7561 cgagggccgc aagggcatgt ttgaccagga tgcgcgtcac gaatttttcc ggcggggcca
     7621 ccgaatcctc gaggtcgaga atg
//
LOCUS       DAOPWU010000161         7606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9659_length_7606_cov_4.843862, whole genome shotgun sequence.
ACCESSION   DAOPWU010000161 DAOPWU010000000
VERSION     DAOPWU010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9659_length_7606_cov_4.843862"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PKM23_09090"
     CDS             complement(<1..1126)
                     /locus_tag="PKM23_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNY91668.1"
                     /translation="MPHVMLRILRPAMPGLFLATILAVTVTAGAGVITVNQAGYQPGD
                     IKIACVSQAADSFYIVDQSDQRIVLEGLLQVRRLQDPSTGMDVYTADFSALTEPGRYA
                     VRVPGVGSSYPFRIMEGVYRDLFAKSLRAFYFQRCGMELKVGLALQYNHPACHLQDGY
                     YHTSTGLSGQSKGTGGWHDAGDYGKYVVNGGITAGTLLLAYETFPARFSADTLKIPES
                     GNGVPDLLDEVRYELEWFFLMQEADGGVHHKLTRTDFEAIIMPHKDTNKRYFMPVSSA
                     ATANFAAVLARAGRIYRPFDPAFADSCTARAQRAWGWLEAHPDIQPAGGFKNPQGVST
                     GEYGDGSDRDERLWAAAELWRTTRSQGCHDWYLSHYASLNL"
     gene            1407..4424
                     /locus_tag="PKM23_09095"
     CDS             1407..4424
                     /locus_tag="PKM23_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY91669.1"
                     /translation="MAKLYRAPLIVLLVLLLLAAQATAQLQETQAEQAFTLARQLYED
                     QLYELAADQFNDFIQKYPHAPQAQEAWFLLGESFFQQRQFEQAITAYRALLERHAALT
                     LRDQAAFRIGQSQVYGNKLTEAKNSLRRFMDDYPSSALYPDARYWYGEALLRGGSKEE
                     AVRHFGEIVADHPQSKVADYALFAIADIREGENRWDEAIAAYNRLLKEYPSSPLAEQA
                     TFRLGMARYGRKEYDEAIGELSRAIVAWPQSRWRGEAQYFIGESFARKGEHEKAAKAF
                     SVISTLPDNRYADDALYGLGLAQLNLNRNEEALAAWQRLLAESGDDDLKAAALFQSGR
                     VEQRRGRSGEALSYFKRVVSDYPRSADAPNALFAHASMLFEKKEFAGARTIFSQIVDR
                     FPKASVAGDAAVMVGECHLGEGYAEAANRVFRQTAGGYDQPEVKAQAIFKLGLSRFQA
                     GEFDLALKNFDDVVRVYGKTAVAPDARFWRGESYFKLSRFNEALADYQGFIRDNPSSP
                     RLAEARYGVGYAALKTGQHQAAAEAFAAAARESDSAPLKCDALMRRGDALMNDKKFAD
                     AVKAYQTAYSSCSDPEHKAEARYQTGLAWFRSGDFDQARRTLEEVTSSWPDSPYAAEA
                     LYLSGRARFRASQFNEAIASFDQLIRSYPGSALVDDATYAIADCHYNLGAYDKAIAQY
                     KKLIEDYPRSELVADALTGLQWSMMQKGETGQADKVVQEYVTRLPDRETAARVLERQA
                     EFFRDNGQYDEAIGQYQKLLSSYGSTAAGAGAWYGIALCRQSQNQPDAAVAAFRQQWE
                     KNPDSPASPRALFAGAQLLAERGEKTQAATWYSKLTTAYPDHELSSAAAYARGVLDVD
                     NQRIDEAERYFKELDSTDKAGAERGLARLGLARVHIGKKQFADAQAILDQLLEQGTPS
                     VSAEAQFLIATILREQKELAKASLAFLKIKYLYPDELNWVVPGIYNAAECNEELGRTA
                     DARRLYQSLVSDYPARSDYAARARARLQALAGK"
     gene            4435..6126
                     /locus_tag="PKM23_09100"
     CDS             4435..6126
                     /locus_tag="PKM23_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91670.1"
                     /translation="MMKTQATRLHLAALLILCSALLAAAQERPLPPARMILPSDSLAA
                     RRTPAKLEADSSRIELPDVVVFGQDRSVRQVESKRRSGDEGPRLLRPDYSSLSIFARR
                     DNSRPLVNAAAAARDRLIWANAAAGSYTSVIADAGYSGKFTWGSARFSGWLDRSNGAF
                     NNSRHAGGGVAATATTPLRPELHGRAQAEMSWLSRGLHGAVLPDLSRKANHSLLAGEV
                     DFAIDPLSSARAGLQIAGAGISTDSSSRELLQSGEFVIGLHGSYQRQMERFTLRAHAA
                     YTRESLSADPDSLDDTAGFSQIRAEVQTSLLRNLQLTAALGYQDFIRGRFAPSLRLAF
                     IPNDRWGITAAAWAGLRHVSIRERLLENPYLSHGVPLAADDSPLSLQVQSDFRPVGSM
                     VFHFGLFHGRYDQLNYWQRDDESGLIGLYQVEKIRLTELQAGVRVGLHAGMMLQGELH
                     LNSDHLEEAVGQGDDDRVPYRPDYRARASLGMPLPWQLQLNAQLEMVGERRRGLDMEG
                     RLPEYVLLNAGVGRKFGRHLYADLAALNILDRRYVIWEGYDEPGIQFMAKARWSY"
     gene            6168..6785
                     /locus_tag="PKM23_09105"
     CDS             6168..6785
                     /locus_tag="PKM23_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNY91671.1"
                     /translation="MSLWDMFTRGGSMMIPILICSIIAVIIVIERYLSLRKIRINSRK
                     FTLQIRSLLSHQRVDEAILLCKETPGPIAGVAKAALVKKDRPREEIKEAIQSAGQAEV
                     FHLEKYMGILATVAGVAPMFGFLGTVTGMIKAFMQIQSRGGNVDASVLAGGIWEALIT
                     TAAGLIVGIPALIAYNWIQGKVEHHVFEMQESSNEFLDMLQEKEA"
     gene            6785..7186
                     /locus_tag="PKM23_09110"
     CDS             6785..7186
                     /locus_tag="PKM23_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNY91672.1"
                     /translation="MIFKTTRKRIISYAAISLTDIILLLLIYFLLSSSFIVQPGIKVT
                     LPRAAAGEVDKADKIYLTVTKSEQLFLNGELVSKDVLGARLTALLRIAADKTVIIRAD
                     KDLTLEATVQVIDIAKLAGAEKFLIATEPGE"
     gene            7195..>7606
                     /locus_tag="PKM23_09115"
     CDS             7195..>7606
                     /locus_tag="PKM23_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91673.1"
                     /translation="MDSLQEISPELRRSILFSILFHLALLLIFLLVTVRVDSLPRSFA
                     EVAFISGTAGGRVTPVSSVEEPAPAAAAESERVPVSRPAAARPASPKPKPEPAPSRIA
                     TASRTGAEEQQRTPVTPPKRRMLEDEEPLISRQEE"
BASE COUNT         1465 a         2585 c         2244 g         1312 t
ORIGIN      
        1 agagattgag cgaggcgtaa tggctgagat accagtcatg acagccctgg ctgcgggtag
       61 tgcgccagag ttcggccgcg gcccagaggc gctcatcgcg gtcgctgccg tctccgtatt
      121 ccccggtgga gacgccctgt ggattcttga atccgcctgc cggctggatg tcgggatggg
      181 cctccagcca tccccaggcg cgctgcgccc tggcggtgca ggaatcggca aaagccggat
      241 cgaaggggcg gtagatgcgc ccggcccggg ccagtacggc ggcgaaattg gcggtcgccg
      301 cgctcgagac cggcatgaaa taacgcttgt tggtgtcctt gtgcggcatg atgatggctt
      361 cgaaatcggt gcgggtcagt ttgtgatgga ccccgccatc ggcctcctgc atgagaaaaa
      421 accactccag ttcatagcgc acctcatcga gcaggtcggg cacaccgttg ccactctcgg
      481 gaattttcag ggtatcagcg ctgaagcggg cgggaaaggt ctcataggcc agcagcaggg
      541 ttccagcggt gatgccgccg ttgacgacat acttgccata gtccccggca tcatgccagc
      601 cgccggtccc tttcgactgt ccgctcaagc cggtgctggt atgataataa ccatcctgaa
      661 gatgacaggc ggggtgattg tactggagag cgaggcccac cttgagctcc atgccgcagc
      721 gctggaaata aaaggcccgc agggatttgg caaaaagatc gcggtaaact ccctccatga
      781 tgcgaaaggg ataggagctg cccacgccgg ggacgcgcac ggcataacgg ccgggctccg
      841 tcagggcgct gaaatcggcc gtatagacat ccatccccgt ggagggatcc tgcagccgcc
      901 gtacctggag caggccttca agcacgatgc gctggtcgct ctgatccaca atataaaaac
      961 tgtcggcagc ctggctgacg caggcgattt tgatatcgcc cggctgatag ccggcctgat
     1021 tgacggtgat cacccccgct cctgccgtca cagtcacggc aagtatggtg gccaggaaaa
     1081 gaccgggcat ggccggccgc aggatacgca gcatcacatg aggcatggca taactccttc
     1141 aggtatgagt gctacaagtt aacaagtctg gtgtcaattt caagcacaat ttggggcttg
     1201 ctgccgaaga aattgcatct gccggctgcg atgcgtcgcc tgtcacaaaa taactacaat
     1261 acccttgctt ttttttcata aaattgctaa aattattata tttatcgtaa aataatccgc
     1321 tgcggcggcg gcccgggcat ccggaacttg tgcgcccggc aagggttaga tcattaatac
     1381 tccgtccatt ttgagaaatc gaagatatgg caaaacttta ccgggcaccc ctcatcgtgc
     1441 ttctggtcct gctcctcctt gcagcccagg ctaccgccca gctccaggag acgcaggctg
     1501 aacaggcctt caccctcgcc cgccagctct atgaggacca gctttacgaa ctcgccgccg
     1561 accagttcaa cgatttcatc cagaaatacc cccatgctcc acaggcccag gaggcctggt
     1621 tcctcctcgg cgaaagtttt ttccaacagc gccaattcga gcaggccata accgcctacc
     1681 gcgccctgct cgagcgccat gccgccctga ccctcaggga ccaggctgcc ttccgcatcg
     1741 gccagtcgca ggtctacggc aacaagctca ccgaggccaa aaacagcctg cgccgcttca
     1801 tggacgacta tccatccagc gccctctatc cggatgcccg ctactggtat ggtgaggctc
     1861 tcctgcgcgg cggcagcaag gaagaggcgg tccgccactt cggcgagatc gtcgcggacc
     1921 atccccagag caaggtcgcg gactatgccc tcttcgccat cgccgacatc cgtgagggag
     1981 agaatcgctg ggatgaggcg atcgccgcct acaaccggct gctgaaggag tacccctcca
     2041 gtccgctcgc cgagcaggcc accttccgcc tcggcatggc ccgctatggc cgcaaagagt
     2101 acgatgaagc gatcggagag ctttcgcgcg ccattgtcgc ctggcctcaa agccggtggc
     2161 gcggcgaggc gcagtatttc atcggcgaat ctttcgcccg caagggggaa cacgaaaagg
     2221 cggccaaggc cttcagcgtg atttctaccc tgcccgacaa ccgctatgcc gacgacgccc
     2281 tctacggtct tggtctcgcc cagctcaacc tgaacaggaa cgaagaggcc ctggccgcct
     2341 ggcagcgcct tctcgctgaa agcggggacg atgacctcaa ggcggcggcc ctcttccaga
     2401 gcggccgggt ggagcagcgc cgcggacgga gcggggaggc cctgtcgtac ttcaagcggg
     2461 tggtgagcga ttatccacgc agcgccgatg cccccaacgc cctcttcgcg cacgcctcca
     2521 tgctcttcga aaagaaagag ttcgccggcg cgcgcaccat tttcagccag attgtcgacc
     2581 gtttccccaa ggcgtcggtg gccggggatg ccgccgtgat ggtgggcgaa tgccatctcg
     2641 gcgagggcta cgccgaggcc gccaaccggg tcttccgcca gaccgccggc gggtacgatc
     2701 agcccgaggt caaggcccag gccatcttca agctcgggct ttcgcgcttc caggccgggg
     2761 aattcgacct cgccctgaag aattttgacg acgttgtgcg ggtctatggc aagactgcgg
     2821 tcgcaccgga tgcccggttc tggcgcggcg agagctattt caaactaagc cgcttcaacg
     2881 aggccctcgc cgactatcag ggcttcatcc gcgacaatcc ctcctccccc cgcctcgccg
     2941 aggcccgcta cggagtgggc tacgccgccc tcaagaccgg ccaacaccag gccgcagccg
     3001 aggccttcgc cgccgccgcc agggagagcg actcggcacc cctcaaatgc gacgctttga
     3061 tgcggcgcgg ggacgccctc atgaacgaca aaaagttcgc cgatgccgtc aaggcctacc
     3121 agaccgccta cagcagctgc agcgacccgg agcacaaggc cgaggcccgc taccagaccg
     3181 gtctggcctg gttccgcagc ggcgatttcg accaggctcg ccgcaccctc gaggaggtga
     3241 ccagcagttg gccggattcg ccctacgccg ccgaagccct ctatctgagc ggccgcgccc
     3301 gcttccgcgc cagccagttc aatgaggcga tcgcctcctt cgatcagctc atccgcagct
     3361 atcccgggag cgcgctcgtc gatgacgcca cctacgccat cgccgactgc cactacaatc
     3421 tgggcgccta cgacaaggcg atcgcccagt ataaaaaact catcgaagat tatcccagga
     3481 gcgaactcgt cgccgatgcc ctcaccgggc tgcagtggtc gatgatgcaa aagggcgaaa
     3541 ccggccaggc cgacaaagtc gtgcaggaat atgtcacccg actgcccgac cgcgagaccg
     3601 cggccagggt gctggagcgc caggccgaat tcttccgcga taacggccag tacgacgagg
     3661 ccatcggaca gtatcaaaag ctgctctcct cctacggcag caccgccgcc ggcgccggcg
     3721 cctggtacgg catcgctctc tgccgccaga gccagaatca gcccgatgcc gctgtcgccg
     3781 ctttccgcca gcagtgggaa aaaaatccgg actctccggc atcgccccgc gccctcttcg
     3841 ccggggcgca gctgcttgct gaaagaggcg agaaaaccca ggccgcgacc tggtacagca
     3901 agctgaccac cgcctatccc gatcacgagc tgagttccgc cgcggcctat gcccgggggg
     3961 tgctggatgt cgacaaccag cggatcgatg aggccgagcg ttatttcaag gagctcgaca
     4021 gcacagacaa ggctggggcg gagcgcggac tggcccggct cgggctggcg cgggtccata
     4081 tcggcaagaa gcagttcgcc gacgcccagg ccatcctcga tcaacttctt gaacagggca
     4141 cgccctccgt cagcgccgag gcccagttcc tgatcgccac catcctgcgc gagcagaaag
     4201 agctcgccaa ggccagcctc gcctttctca agatcaagta tctctatccg gacgaactga
     4261 attgggtcgt ccctggcatc tacaatgccg cagagtgcaa cgaggagctg ggcaggacgg
     4321 ccgacgcgag gcgtctctat caatccctgg tcagcgatta ccccgccaga tcggactatg
     4381 ccgcccgcgc gcgtgctcgt ctgcaagcgc tggcaggaaa gtgaggcggc ggcgatgatg
     4441 aaaacccagg ccacccgact gcacctcgcc gcgctcctca tcctctgcag cgccctgctc
     4501 gcagccgccc aggagcgccc ccttcccccg gcgcgcatga tcctgccctc cgactcgctg
     4561 gcggcccggc ggacccccgc caagctggag gccgacagca gccgtatcga gcttcccgat
     4621 gtggtcgtgt tcggccagga ccgcagtgtg cgccaggtcg aaagcaaacg ccgttccggt
     4681 gacgaggggc cgcgcctgct ccggcccgat tacagctccc tctccatctt tgcacggcgg
     4741 gataacagcc gccccctggt caatgccgcc gcggctgccc gtgaccggct gatttgggcc
     4801 aacgcggcag cgggctctta caccagcgtc atcgctgatg caggctattc cggcaagttc
     4861 acctggggct cggcacgatt ctcgggatgg ctggaccgca gcaacggcgc cttcaacaac
     4921 agccgtcatg ccggcggcgg agtggcggct acggccacca cccccctgcg tccggagctg
     4981 cacggacgcg cacaggccga aatgagctgg ctcagccgcg gcctccacgg cgctgttctc
     5041 cccgacctca gccgcaaggc caaccacagc ctcctggccg gggaggttga tttcgccatc
     5101 gatcccctct ccagcgcccg cgccggtctc cagatcgccg gggccggcat ctccaccgac
     5161 agcagcagcc gcgagctgct gcagagcggt gagttcgtca tcgggctgca cggcagctat
     5221 cagcgccaga tggaacgctt caccctgcgc gcccatgccg cctacacgcg cgaatccctc
     5281 tccgccgatc ccgacagcct cgatgacacc gcaggtttca gccagatccg cgccgaggtg
     5341 cagacctccc tcctgcgcaa cctccagctc accgccgccc tcggctacca ggatttcatc
     5401 cgcggccgct tcgccccctc cctcaggctg gccttcatcc ccaacgaccg ctggggcatc
     5461 accgccgccg cctgggccgg gctccgccac gtcagcatcc gcgagcgtct gcttgagaac
     5521 ccctatctct ctcatggcgt gcccctggcc gccgacgact cccccctctc gctgcaggtg
     5581 cagagcgatt tccgcccggt cggctcgatg gtctttcact tcggcctctt ccatgggcgc
     5641 tatgatcagc tcaactactg gcagcgggac gatgagagcg gtttaatcgg actttaccag
     5701 gtcgaaaaga tccgtctcac cgaactccag gccggggtcc gcgtggggct gcacgccggc
     5761 atgatgctcc agggtgaact ccatctcaac agcgaccatc tcgaggaagc agtcggccag
     5821 ggcgatgatg accgtgtccc ctaccggccg gactacaggg cgcgggcctc cctcgggatg
     5881 cctctgccct ggcagctcca gctcaatgcc cagctcgaga tggtcggcga gcgccgccgc
     5941 ggtctcgata tggaggggcg gctgcccgaa tacgtgctcc tcaacgcggg cgtgggccgg
     6001 aaattcggcc gccatctcta cgctgatctg gcggccctca atatcctcga ccgccggtat
     6061 gtgatttggg aaggttatga cgaaccgggt atccagttca tggccaaagc aaggtggtct
     6121 tactaactca gctgcaagta ccaataaaac tgaatggagg caacagcatg tcgttgtggg
     6181 atatgttcac cagaggcgga tcgatgatga tccccatcct gatctgttcc atcatcgcgg
     6241 tgatcatcgt catcgagcgt tatctctccc tgcgcaagat ccgcatcaac tcccgcaaat
     6301 tcaccctgca gattcgcagc ctgctcagcc accagcgggt ggacgaagcc attcttcttt
     6361 gcaaggagac cccgggtccg atcgccggtg tcgccaaggc ggccctggtt aaaaaggacc
     6421 ggccgcgcga ggagatcaag gaggcgatcc agagcgccgg acaggcggag gtcttccacc
     6481 ttgagaaata catgggcatc ctggcgaccg tggccggcgt ggcgcccatg ttcggctttc
     6541 tcggcacggt caccggcatg atcaaggcct ttatgcagat ccagtcccgc ggcggcaacg
     6601 tcgacgccag cgtcctcgcc ggcggcatct gggaggccct catcaccact gcggcgggac
     6661 tcatcgtcgg catccccgcc ctcatcgcct acaactggat ccagggcaag gttgagcatc
     6721 acgtcttcga gatgcaggaa agctccaacg aattcctcga catgctccag gagaaggagg
     6781 cgtaatgatc ttcaagacta cgcgcaaacg catcatcagc tatgcggcca tcagcctcac
     6841 cgacatcatc ctgctgctgc tgatctattt tctcctctcc tcctccttca tcgtgcagcc
     6901 gggcatcaag gtgaccctgc cgcgcgccgc ggcaggcgag gtggacaagg cggacaagat
     6961 ctatctcacc gtgaccaaat cggagcagct cttcctcaac ggcgagctcg tgagcaagga
     7021 tgtgctgggg gctcgcctga ccgcgctgct ccgcatcgct gcggacaaga ccgtgatcat
     7081 ccgcgcagac aaggacctga ccctcgaggc gaccgtacag gtgatcgata tcgccaaact
     7141 ggccggcgcg gagaaatttc tcatcgccac tgaacccggc gagtgaggtt gaagatggac
     7201 tctttgcagg agatctcacc ggaactgcgc cgcagcatcc tcttttccat cctcttccat
     7261 ctcgcgctgc tgctgatttt tctcctcgtc accgtgcggg tggactcgct tcccaggagc
     7321 ttcgccgagg tggccttcat cagcggtacc gcgggagggc gggtcacacc cgtgagcagc
     7381 gtcgaggagc cggcgcccgc ggcagcagcg gaatcggaga gggttccggt ctcccggccg
     7441 gccgctgcgc ggccggcgag ccccaaaccc aaacctgagc cggctcccag ccgcatcgcg
     7501 accgccagcc gcaccggcgc cgaagagcag cagcgcaccc ccgtgactcc cccgaaacgg
     7561 cgcatgctgg aggatgagga gcccctcatc agccgccagg aagagg
//
LOCUS       DAOPWU010000162         7585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9692_length_7585_cov_5.081009, whole genome shotgun sequence.
ACCESSION   DAOPWU010000162 DAOPWU010000000
VERSION     DAOPWU010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9692_length_7585_cov_5.081009"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PKM23_09120"
     CDS             <1..600
                     /locus_tag="PKM23_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HNY91674.1"
                     /translation="ASEIPELPIFVDSPLSLNATEVFRAHPECYDEETADFLESTNDV
                     FGWNRLRYIRTAEESKKINEIKSPCVIISASGMCEAGRILHHLRNNIEDERNTILVVG
                     FMAENTLGRRLVEREPTVKIFGEEFTLAAEVVKLNAFSAHADQDELVTWISHFNREHL
                     EHLFILHGEYDQSQALAHALGGLGLNEVTIPERGEWLEV"
     gene            complement(696..1565)
                     /locus_tag="PKM23_09125"
     CDS             complement(696..1565)
                     /locus_tag="PKM23_09125"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNY91675.1"
                     /translation="MKVPLNKRTLFSPLVLVLLLITAITASAQRIEAVVTTDLRTLPL
                     DRQEKLRDFKEKVEHYLNAYTWTNDPWNTVLYLDIQIILEDMSTSAEERYRGQIVMSN
                     KYDMQLSDKRWRFAYQSTDMLNHDESTLNSFTIMLDYFVYVFLGMEFDKWATLGGTEY
                     FQKAKTIAEQAKFGLGRYIEGWDRRVEQITYLTSDQHVPFREMVDYYFYGLSLVKEDN
                     VKARQFIARAVEMLDKIISKDPENEYAKMFLNAHYIEMVEVFRRAMDKSPLRTLMVID
                     PAHAQVYKNILEK"
     gene            1741..2508
                     /locus_tag="PKM23_09130"
     CDS             1741..2508
                     /locus_tag="PKM23_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91676.1"
                     /translation="MGAIGEPPPGGRWLAMGGSGVASTALREAWAQNPAALPFAPAWH
                     WSSSAGRPFGLAELTSWQCGGGLARKNRGLALAAGGQGIDLCRETTLALAVGRAFSNG
                     AAFGIALRRVGIAIERYGSASTLLCDFGGQLRCSAELRIGFCLHNPFAAAIGRCREEI
                     PPSLQSGIALQLSPSASLSTDLYFEKGLPLELRCGAEQLLGEVFALRAGFSSAGWRLA
                     GGFALRFRRISVEYGMMTHSWLGLSHQCSLCFHPGRR"
     gene            2527..4494
                     /locus_tag="PKM23_09135"
     CDS             2527..4494
                     /locus_tag="PKM23_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNY91677.1"
                     /translation="MRCHVLFLCLLLPALASGQEEDWEMLVDPELEEERAEELLEFFE
                     WRRSDPLDINHARLGDWLQFPWVDAVAARAILAERRRRGSFRRLSDLESIAELSPVAY
                     RRLLPYLRCSAPRGSSWKTEGRHRWGLVWPADSTGGADRFEGDGSRSYHRFGLGLADR
                     LQAGVVCEKDPGESSPADLLRAGGMWQMPWLSGRLCLGSFTLESGRGLLFARPGSWGR
                     TTDPVAALRPREAVLRPSLSAAESGGRQGAALAFEQGGAELFIFTCRGFWDATVEEGR
                     VTALQWSGLHRTGTEAAKKGRLGADEYGVMIQRRFGASLRLGAAWQEARYSLPLAPDS
                     SLENIHRFAGQENWNGGFEARLSLAGADAFAEWACSRSGGSALEAGVTVQAGRVEGVA
                     RLRSCSPDYHRLLGSDSEEPRNEETLYLALQAELPRLRLAAWYDVSRNPASAWRRPMP
                     LIPAHETAVMAAWQPLTDLSLFHRLRWRSWSLIENSTGPFGNSVKRWRDRLLWSALGQ
                     MEYKTGGLAWRVRLEYRRFSIGRAGTELPAVPDSSGWMLCQQLSLELGRGSRVTARYL
                     FHDTPCYETRIYLYENDLPGTLSLPALYGRGCRAFVLLHLKLTRALAFSIKWSWRPGD
                     SGGSGTGQRRRPGPDEQSLGMQMDWRLGGGR"
     gene            4587..5141
                     /locus_tag="PKM23_09140"
     CDS             4587..5141
                     /locus_tag="PKM23_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91678.1"
                     /translation="MAMQKMAQSLALRKYRKRAEKQARSLDFSAHLEGVERVLVCLPE
                     AAEEYARILPALAGIRKAFPRAHVTLLQSSAIPVSTEMVRGFQLVAWGAADLDRLGGP
                     GVLFRRRLFAESFGLALDLNQEPRFFTLAVMMESGAPVRAGYAGSMRDELYTFLVRPG
                     TQNAVQALQTLLTYLGQPESERRR"
     gene            5479..6567
                     /locus_tag="PKM23_09145"
     CDS             5479..6567
                     /locus_tag="PKM23_09145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNY91679.1"
                     /translation="MSGDSEEKTSGKGSRLGFIGERRANRRIRVLRQVVGWTRLLARL
                     DGSDEAFISLAPFFKRYFAVDGFALWVREPGRWQLVFAWPPVLEEVTPRQLSWRGGQP
                     ATPREAFATPPGMTCRLDLLIAQQSSLPLGWLTLLRQADESFTVRERNLLHKLMGLFA
                     AHLSAQVRVRDAEAQAMTDVLTGIWNRRYFEERYPAELQRARRYRRSLAVLMIDIDCF
                     KSYNDTHGHLAGDQALQAVAQLLKGNLRTSDILCRYGGEEFVVLLPESDREHALLAAE
                     KLRRAVAAELLPISRSQTGGRLTISIGVSACPENGEGEMELLHQADRALYTAKKGGRN
                     RVMGTEVTARSALALEAAQQGDRPAFVK"
     gene            complement(6511..>7585)
                     /locus_tag="PKM23_09150"
     CDS             complement(6511..>7585)
                     /locus_tag="PKM23_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNY91680.1"
                     /translation="PGERSDQNSHGTMVLSIIAANKTGTLIGGAPRAEFILAKTEDVA
                     QEIRGEEDNWIAAAEWAEALGADIFSTSLGYIDWYTYADMDGDTAPITRAADLAAGKG
                     VVVVVAAGNEGNDIWRYISAPADGDSVLAVGAVDNTGKVAAFSSRGPTADGRIKPDVM
                     ARGIAVACIDIPPAEGKGSGYRSVNGTSAACPLAASAAALLLCANPELTPMELIDALR
                     RSATLNLTPDVDYGWGVIQTLAAVESTASVNRPVIIPERPALAGIYPNPAAMALNSSM
                     TLVFDLVKAEEVDVRLYNVRGQEVGRLFQGKVPAGTGRQVRVRWPAALNSFGSGLYFV
                     VVKSGSLHESRAVTLLRGLEGER"
BASE COUNT         1246 a         2418 c         2466 g         1455 t
ORIGIN      
        1 gcgagtgaaa tcccggagct gccgatcttt gtagattcac cgctctcact caatgccacc
       61 gaggttttcc gcgcccatcc cgaatgctac gacgaggaga cggcggactt tctcgagagc
      121 accaacgacg tctttggctg gaatcgtctc cgctacatcc gtaccgccga ggagtccaaa
      181 aagatcaacg agatcaaatc cccctgcgtg atcatctcgg cctcgggcat gtgcgaagcc
      241 ggccggatcc tgcaccactt gcgcaacaat atcgaggatg agcgcaacac catcctggtg
      301 gtcggattca tggccgagaa caccctcggc agacggctgg tcgagagaga gccgacggtc
      361 aagatcttcg gggaagagtt cactctggcc gccgaggtgg tcaaactgaa cgcctttagc
      421 gcccacgccg accaggacga gctggtcacc tggatcagcc atttcaaccg tgagcatctc
      481 gagcatctct tcatcctgca cggagagtat gaccagtccc aggccctggc gcatgccctg
      541 ggcggactgg gcctgaacga ggtcaccatc cccgagcgcg gggagtggct ggaggtctag
      601 ctggatatga aaaagcccgg cgctctgtca gcaagccggg cttgagcagc ggcctccgcc
      661 tgctcagcag gaggggggcc gaatgcagca agagcctatt tttcgaggat gtttttataa
      721 acctgggcgt gagccggatc gatgaccatc agggtgcgca ggggggattt gtccatggcg
      781 cggcggaata cctccaccat ttcgatatag tgggcgttca ggaacatctt ggcatattca
      841 ttttcggggt ccttggagat gattttgtcg agcatctcca ccgccctggc gatgaactgg
      901 cgcgccttga cattgtcctc cttgaccagc gagagaccgt aaaagtaata gtccaccatc
      961 tcccggaagg gaacatgctg atcactggtt agataggtga tctgttccac cctgcgatcc
     1021 cagccctcaa tgtaccgacc cagaccgaac ttggcttgtt ctgcgatggt cttcgccttt
     1081 tgaaaatact ccgtgccgcc cagagtcgcc catttgtcga attccatgcc caggaaaaca
     1141 tagacgaagt aatcgagcat gatggtgaag gagttgagcg tgctttcatc gtgattgagc
     1201 atgtcggtgc tttgataggc gaagcgccag cgcttgtcgc tgagctgcat gtcatatttg
     1261 ttgctcatga cgatctggcc ccggtagcgc tcttcggcgc tggtgctcat atcctcaagg
     1321 atgatctgga tgtcgagata gagcacggtg ttccaggggt cgttggtcca ggtataggcg
     1381 ttgagatagt gctcgacctt ttccttgaaa tcacgcagtt tttcctgccg gtccagcggc
     1441 agggtgcgca agtcggtggt cacgaccgct tcgatgcgct gcgcagaggc cgtgatcgcg
     1501 gtgatgagca gcagcactag gacgaggggg gagaagaggg ttcttttatt gagagggacc
     1561 ttcatgaaag actccgaatc cgtgactgta cgccgcccgg cggggtggcg gttgcaaggg
     1621 tgctataata ttcaacattt atccgattta agcaagcatt ttccgctctt ctgtatacgc
     1681 gcgtgggcct tctgccgttc cattcccctc gttcttctgt tcctgctcgc gcccgctgtg
     1741 gtgggggcca tcggtgagcc tccccctggc ggccgctggc tggcgatggg cggctccggc
     1801 gttgccagca cggccctgcg cgaggcctgg gcgcagaacc cggccgccct gcccttcgca
     1861 ccggcctggc actggagcag cagcgccggc cgccccttcg gcctggccga gctgaccagc
     1921 tggcagtgcg ggggcggcct cgcccgcaaa aaccggggcc tggccctggc ggccggcggc
     1981 cagggcatcg acctctgccg cgaaactaca ttggccctag ccgtcggccg cgccttctcc
     2041 aacggggccg ccttcggcat cgccctgcgc cgggtgggga tcgccatcga acgttacggt
     2101 tccgccagca ccctgctttg cgatttcggc gggcagctgc gctgctccgc ggagctgcgg
     2161 attggctttt gcctgcacaa tccctttgcc gccgccatcg gccgctgccg cgaagagatc
     2221 ccgccctccc tgcagagcgg catcgcgctg cagctctcac cctccgcctc gctctccact
     2281 gatctctatt tcgaaaaggg gctgcccctc gagctgcgct gcggcgccga gcagctgctc
     2341 ggggaggttt tcgcactgcg cgccggtttt tcttccgccg gatggcgcct ggccgggggc
     2401 tttgccctgc gtttccggcg catctccgtc gagtacggca tgatgaccca ttcctggctg
     2461 ggactgagcc accagtgctc cctctgtttt catcccggcc gccgctgagg ggagggagat
     2521 gaagccatgc gctgccacgt cctctttctc tgcctcctcc tgcccgcgct ggcttcgggc
     2581 caggaggagg attgggaaat gctcgtcgat ccggagctgg aggaggaacg ggctgaggaa
     2641 ctgctcgagt tctttgagtg gcgccggagc gatcctctcg acatcaatca cgccaggctc
     2701 ggcgactggc tgcagttccc ctgggtcgat gcggtggcgg ccagggctat cctcgccgaa
     2761 cggcggcggc ggggctcctt ccgccggctc tccgacctcg agtccatcgc ggagctttca
     2821 ccagtggcct atcgccggct gctcccctat ctgcgctgtt ccgcgccgcg ggggagcagc
     2881 tggaagacgg agggccgcca ccggtggggg ctggtctggc cggccgattc caccggcggt
     2941 gccgaccgct ttgaggggga cggctcgcgc agctatcacc ggttcggcct ggggctcgcg
     3001 gaccgcctgc aggccggagt ggtttgcgag aaagacccgg gcgagtcctc gccggccgat
     3061 ctgctccggg cgggcgggat gtggcaaatg ccctggctga gcggccggct ctgtttgggc
     3121 tccttcaccc tcgaaagcgg ccgcggactg ctttttgccc gccccgggag ttggggaaga
     3181 accactgatc cggtggccgc gctgcgcccc cgcgaggcgg tgctgcgtcc ctccctctcg
     3241 gcggccgaga gcggaggccg gcagggggcc gcccttgcct tcgagcaggg tggagccgag
     3301 ctttttatct ttacctgccg cggcttctgg gacgccaccg tggaggaagg gcgggtgact
     3361 gccctgcagt ggagtgggct gcaccggacc gggacggagg cggccaaaaa ggggcggctg
     3421 ggcgctgatg agtacggagt gatgatccaa cgccggttcg gcgcctcgct gcgccttggc
     3481 gccgcctggc aggaggcgcg ctactccctc cccctggcgc cggattcatc cctcgaaaac
     3541 attcatcgct ttgcaggaca ggagaactgg aacggcggat tcgaggcgcg cctcagcctc
     3601 gcgggggcgg acgctttcgc ggaatgggcc tgcagccgca gcggaggatc ggccctggag
     3661 gcgggtgtga ctgtgcaggc cggcagggtg gagggggtcg cgcggctgcg ctcctgcagt
     3721 ccggattatc accgtctcct cggctccgac agcgaagaac cccgcaacga agagactctt
     3781 tatctggccc tgcaggcgga gctgccgcgg ctgcgactgg cggcctggta tgacgtgagc
     3841 cgcaacccgg ccagcgcctg gcgccggccc atgcccctga tccccgctca cgagacggcg
     3901 gtgatggccg catggcagcc gctcaccgat ctcagccttt ttcaccgttt gcgctggcga
     3961 agctggtcgc tgattgaaaa cagcacgggt cccttcggca acagcgtgaa acgctggcgc
     4021 gaccgcctgc tctggagcgc cctcggtcag atggagtaca agacgggagg gctggcctgg
     4081 cgggtgcgcc tcgaataccg ccgcttcagc atcggcaggg ccggcacgga actgcccgca
     4141 gtgccggaca gcagcggctg gatgctctgc cagcagctca gcctggagct ggggcgggga
     4201 agccgtgtga cggcgcgcta tctttttcat gacaccccct gctatgaaac ccgcatctat
     4261 ctctacgaga atgatctccc tggaaccctc agccttccag ccctttacgg gcgaggctgc
     4321 cgtgcctttg tactccttca tctcaagctg acccgcgccc tggctttctc gatcaagtgg
     4381 agctggcgcc ccggcgatag cggcggatcg ggcacggggc agcgcaggcg gcccggcccg
     4441 gacgagcaga gcctcggcat gcaaatggac tggcggctgg gtggtggacg ctagtgcttg
     4501 tggcccggat gcgggaatta gctgttgacc ggggcgttaa atttttgtac atttatcttc
     4561 cataaaaagc aggacaaggc aatcccatgg cgatgcaaaa aatggcgcag tccctcgcgc
     4621 tgagaaaata ccgcaagcgg gcggagaagc aggcccggtc actcgatttc agcgcccatc
     4681 tggagggggt cgagcgcgtg ctggtctgtc tccccgaagc cgcagaggaa tatgcccgta
     4741 tactccccgc tctggccgga atccgcaagg cctttccgcg cgcccatgtc actctgctgc
     4801 agagcagtgc catcccggtt tcgaccgaga tggtgcgggg ttttcaactg gtggcctggg
     4861 gggcggcgga tctggaccgc ctgggcggtc caggcgtttt attccggcgc cgtcttttcg
     4921 cggagtcctt cggcctcgcc ctcgatctca accaggagcc ccgctttttc actctggcgg
     4981 tgatgatgga gagcggcgcc ccggtccggg ccggctatgc cggctcgatg cgggatgagt
     5041 tatatacctt tctggtgcgt cccggcaccc agaatgccgt gcaggccttg cagacgctgc
     5101 tgacctatct cggccagccc gagtccgagc gacggcgctg accggcgcgc tcgaggtggt
     5161 cgctgcgcgg ctccttcgtc gctgtttatc caccctgttc cctccctgcg tcagccctcc
     5221 cgggctggcg atctctgccc gaaagtccat cgttctggat gaactccggc caccccgttt
     5281 catctctttc ttgtaaattc ttattcttcc tcacaaaaag gcaagaaaat ccgggcatca
     5341 tatttgcagt agagaaaaca ccgttacagc gccggaagag ctccggccgg gcggcaggta
     5401 tcctgcagat gatggagctg cagaaagagg ggcggggcgg aaaagcagtg cacggggagc
     5461 attcgaagga gcggctactt gtcgggcgat tctgaggaaa aaacttcggg taaaggcagc
     5521 cggctcggct tcatcggtga acggcgggcg aacaggcgga ttcgggttct gcgccaggtg
     5581 gtcgggtgga cccgtctgct ggccaggctc gacggcagtg atgaggcctt tatttcgctg
     5641 gcccctttct tcaagcgtta ttttgcggtg gacgggtttg ccctctgggt gcgggagccg
     5701 gggcgctggc agctggtctt cgcctggcct ccggttctgg aggaggtgac tccccggcag
     5761 ctctcctggc ggggcggcca gccggccacg ccgcgcgagg cctttgccac gccgccggga
     5821 atgacctgcc gtctggatct cctgatcgcc cagcagagca gccttcccct gggctggctc
     5881 accctgctgc ggcaggcgga tgaatccttc accgtgcgcg agcgcaatct cctgcacaag
     5941 ctgatggggc tcttcgccgc ccacctctcc gcgcaggtgc gcgtgcgcga tgccgaggcc
     6001 caggcgatga ccgatgtcct gaccggcatc tggaaccgcc gctatttcga agagcgctat
     6061 cccgccgagc ttcagcgcgc ccggcgttac cgccgcagcc tggcggtgct gatgatcgac
     6121 atcgactgtt tcaaatccta caatgacacc cacggccatc tggccggcga ccaggccctg
     6181 caggccgtcg cccaactcct taagggcaat ctccgcacct ccgatatcct ctgccgctat
     6241 ggcggcgagg aattcgtcgt cctgcttccc gagagcgacc gggagcacgc cctgcttgcg
     6301 gctgaaaagc tgcgccgggc agtcgctgct gaactcctgc ccatcagccg cagccagacc
     6361 ggcgggcgtc tcaccatctc aatcggagtc tccgcctgcc ccgaaaacgg cgagggcgag
     6421 atggagctgc tccatcaggc cgaccgcgcc ctctatacag ccaaaaaagg gggacgcaac
     6481 cgggtgatgg gcaccgaggt caccgcccgc tcagcgctcg ccctcgaggc cgcgcagcag
     6541 ggtgaccgcc ctgctttcgt gaagtgatcc gcttttaacc actacaaaat agaggccgct
     6601 gccgaaagag ttcagcgcag ccggccagcg caccctgacc tgccggccgg tgccggcggg
     6661 aacttttccc tgaaagagcc tgcctacctc ttgtcccctc acattgtaga ggcggacatc
     6721 gacctcctcc gctttaacca ggtcaaagac gagggtcatc gaactgttca acgccatggc
     6781 ggcgggattg ggatagatcc cggccagggc gggccgttcg ggaatgatga ccggccggtt
     6841 tacggaggct gtggattcca cagcggccag ggtctggatg accccccagc cgtagtcgac
     6901 gtcaggggtg agattgaggg ttgcactccg gcgcagggca tcgatgagtt ccatcggggt
     6961 cagctccgga ttggcgcaga gcagcagggc ggccgcgctc gcagccaggg ggcaggcggc
     7021 ggaggtgccg ttgacagacc ggtagccgga tcccttccct tcagcggggg ggatatcgat
     7081 gcaggcgacg gcgatccccc tggccatcac atccggcttg atgcgtccgt cggcggtcgg
     7141 gccgcgcgag ctaaaagcgg ccaccttccc cgtattgtcc acggcgccca ccgccagcac
     7201 gctgtcgcca tcggcggggg cggagatgta tcgccagatg tcgttgccct cattgcccgc
     7261 ggcgaccaca accacgaccc ccttgccggc ggccagatcg gccgcacgcg tgatcggggc
     7321 ggtgtcgccg tccatgtccg cataggtgta ccagtcgata tagccgaggc tggtgctgaa
     7381 gatgtcggcc cccagggctt ccgcccattc ggcggcggcg atccagttgt cctcctcgcc
     7441 gcggatctcc tgggcgacat cctcggtctt ggcgaggatg aactcggcgc gcggggcgcc
     7501 cccgatcagg gtgcccgtct tgttggcggc gatgatcgag agcaccatgg tgccgtggct
     7561 gttctggtcg ctgcgctcac cgggc
//
LOCUS       DAOPWU010000163         7415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_9980_length_7415_cov_4.347283, whole genome shotgun sequence.
ACCESSION   DAOPWU010000163 DAOPWU010000000
VERSION     DAOPWU010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9980_length_7415_cov_4.347283"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKM23_09155"
     CDS             complement(<1..219)
                     /locus_tag="PKM23_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY91681.1"
                     /translation="MKASILVVEDNEDLCKTIADVMKREGYFVKTAASGEEALTRIEK
                     GLIDLVLLDIKLPRKSGLEVLARIRELQP"
     gene            complement(216..1673)
                     /locus_tag="PKM23_09160"
     CDS             complement(216..1673)
                     /locus_tag="PKM23_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNY91682.1"
                     /translation="MEFDYQELIESVQDMVFAVSPSGGLIYCNRQFALFSGMKKRALA
                     AAPLLDLVHPPDRSRVERMLQETLQGSARTFEARFVGESGTPLYFSTNMTPIREGGHV
                     TGAIGISRDINERMVLEQQITGLKNFQESILKSIQAGLITIDLEGRITSFNAAAGEVL
                     NCSARQAVGSPLEQIVGEEAAGIFLQRVVRGDFPTNRELPLKTPAGYETYIGFTVTPL
                     LDELGDRTGTILSFRDISLIKRMQAEVMRMDRLASLGVLASGIAHEIKNPLAGIKAMA
                     QTLQQDLDPEEESYDYLERIVRQVNRLDELLKTFFDYARPRPPQLKLHRIQDILHEVM
                     TLVDKRLQEKEIRFIPLIPAETPAVFADFHQIQQVILNLIINAIDAMEEGGELVIQTG
                     PSRGTALRSPAAGADPDRRFVEITVTDSGVGIPEENLEVIFDPFYTTKAQGTGLGLSI
                     VYRIIAAHNGEISVSSKLGEGTTFRVILPTEEESK"
     gene            complement(1701..2399)
                     /locus_tag="PKM23_09165"
     CDS             complement(1701..2399)
                     /locus_tag="PKM23_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY91683.1"
                     /translation="MKKGTVLMVDDNLDMLLIGQRIFTKAGFEYLSARSGLEGLERMA
                     AGGVDIVILDYMLLDLNGLQFLETIAAEPAYAPLRAIPVIVLTARTDYLEELDSLFAA
                     NLRAVLTKPFGHRELVNVVENALRLERSLRPLLAAASPARPAGPAPSATATAQSAPAA
                     AVPEARWSDDLRIAAGTIARLTAELRRAQPEGLNEQQRLDLDAVVTSSRRLVRMIDEK
                     FPESEIFTTTTLTA"
     gene            complement(2445..3938)
                     /locus_tag="PKM23_09170"
     CDS             complement(2445..3938)
                     /locus_tag="PKM23_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91684.1"
                     /translation="MSNWIALQLKSRLFRKVFFYTFAATMLPLVLMGFYWLRMTDGGS
                     AVLFPGFFSVVVLALLLAGIVAYFLSRNITLPISDLAKSATEIARGNFSHEIKVLSND
                     EIGRLARLFNYMTTELRRLNNMNLAQIIAERNKTQTIIKNIADGVVVTDPRGKVLLLN
                     ASAERWLGLREKDALEKPLENFIQEEKMLQLLADAGVNNNTTLPPVEITFRPPGEWKP
                     RIFQAHAARVLQEDNELIGIVTVLSDITQQKEIDRMKTELVSMVAHELRSPLTSISGF
                     SELLLDPELNREQQEEYAGIILKESNRLGDLINKFLDISRIESGRIQPKKNALDLVET
                     VQMVVGNNSFLAAKKNIDVEVHPPDEPAEIWADSGMMEQVFINLFSNAVKYSPENTRI
                     DVLVRQNENEVIAEVHDQGYGIPRNAIGKIFDKFFRVTEHEAVRASTGTGLGLALVKQ
                     IIELHGGRITVESEIGKGSVFTVYMPKAHIAQSGLPQNEEDAENIIR"
     gene            complement(3954..4907)
                     /locus_tag="PKM23_09175"
     CDS             complement(3954..4907)
                     /locus_tag="PKM23_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HNY91685.1"
                     /translation="METLNTVKRPLILVVDDLPVNIQLIQNYLAAVGYDTMSAHNGEE
                     ALARIRERQPDLVLLDVMMPRMDGFETCRHLKHSPSTQYIPVIMVTALNELDDKIRGI
                     EAGADDFISKPFNRLELLARVKSLLRVKTLHDQLQVKIRQLEQMKEQLRALAVTDGLT
                     GVYNYRYFRQYLAQEIRRAERHQLAVSLAMLDIDFFKRFNDAHGHLAGDEVLRIVARL
                     MKGHTRAIDVVARYGGEEFAIILPETNKAEARIVAEKVRRLVENFPFPQSNGKSQVHL
                     TVSVGVATFNDDARSADSLIHVADQRLYRAKAEGRNRVVDK"
     gene            complement(5217..6764)
                     /locus_tag="PKM23_09180"
     CDS             complement(5217..6764)
                     /locus_tag="PKM23_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNY91686.1"
                     /translation="MLYSRKKRIAALTFGGLVVLAVLWIGPARIIHAAQSTYNKMMVL
                     TMVLDKIERFYVDDRNPDELIDRAIDGILGALDPHSIYLTPDEFTEYKGAFLGYQGIG
                     LKYNRLPEKLLVASVVPGGPAAEAGIRPGDKIMRISGKMTQYITDTELPALLQEPVVR
                     LEIERDGVRDLLPFAISKRPVVPQTVSSAFMLDDSVAFIKLERFIQTTPAEFDQAWSR
                     LRAAKPSYLILDLRDNMGGDLQAGVKIADRFLSAGKMISYTTGRGPGANMEFRAGTEN
                     KLPALPVIVLINGGSASDAEIVAGALQDWDRALIAGKPSFGKALVQSEFTFPDGSAML
                     LTTARFYTPLGRSLFRTGQARPQNGGRSTGREGATLRTPGGRLLTANGSIVPDVDLSA
                     DSLNYSPALRRLLAARENPLAIFSETYSAAHPELARDREGFIRNFEPPESLLRDFQQQ
                     ARKMGVNFSAQDLSRNREGVRLLLKREIAAMMWGEEVRTQITLLADPQVRDCSGLWRR
                     ARQLTGR"
     assembly_gap    7006..7105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1392 a         2142 c         2304 g         1477 t
ORIGIN      
        1 cggctggagc tcacggatgc gcgccagcac ctccagacca cttttgcggg gcagcttgat
       61 gtcaagcagg accaggtcga tcagcccctt ctcgatccgc gtcagcgcct cctccccgct
      121 cgctgcggtc ttgacgaagt agccctccct cttcatcaca tcagcgatgg ttttgcagag
      181 atcctcatta tcctcgacga cgagaatgct cgccttcatt tactctcttc ctccgtaggc
      241 agtatgacgc gaaaagtggt cccctcaccg agtttgctgg agacgctgat ctccccgttg
      301 tgtgcggcga tgatgcggta gacgatcgaa agcccgaggc ccgtcccctg cgccttggtg
      361 gtgtaaaagg ggtcgaaaat aacctccagg ttctcctccg gaatgccgac ccccgagtcc
      421 gtcacggtga tctcgacaaa acggcggtcc ggatcggccc cggccgcagg cgagcgcagg
      481 gcggtgccgc ggctgggtcc ggtctgaatc accagctccc ccccctcctc catggcgtcg
      541 atggcattga tgatgaggtt gaggattacc tgctggatct ggtgaaaatc agcaaagacc
      601 gccggagttt cggccgggat caacggaatg aagcggatct ccttctcctg gaggcgcttg
      661 tcaaccaggg tcatgacctc gtgaaggatg tcctggatgc gatgcagctt gagctggggc
      721 ggccgcggcc gggcatagtc aaaaaaggtc ttgagcagct cgtcgaggcg gttgacctgg
      781 cggacgatgc gctcgagata gtcgtagctc tcctcctccg ggtcgaggtc ctgctgcagg
      841 gtctgggcca tggccttgat gccggcaaga ggattcttga tctcgtgggc gatgccggag
      901 gcgaggacac cgagggaggc aaggcggtcc atgcgcatca cctcggcctg catgcgtttg
      961 atcagggaga tgtccctgaa gctgaggatg gtgccggtgc ggtcaccgag ctcgtcgagc
     1021 agaggagtga ccgtaaagcc gatataggtt tcatagccgg cgggggtttt gagaggcagc
     1081 tcgcggttgg tggggaaatc accacgcacc acccgctgca gaaagatgcc cgctgcctct
     1141 tcgcccacaa tctgttcgag cgggctgccg acggcctgac gggcgctgca gttcagcacc
     1201 tcaccggcgg cggcgttgaa ggaggtgatc cgcccctcca gatcgatggt gatcagcccg
     1261 gcctggatgc tcttgaggat gctctcctga aagttcttca gcccggtgat ctgttgttcg
     1321 aggaccatgc gctcgttgat gtcgcgggag atgccgatcg ccccggtgac atgacccccc
     1381 tcgcggatgg gggtcatgtt ggtcgagaag taaagggggg tgccgctctc accgacaaaa
     1441 cgcgcctcga aggtgcgcgc cgagccctgc aaggtctcct gcagcatcct ttccacccgg
     1501 ctgcggtcgg gaggatggac gagatcgagc agcggggcag cggcgagggc cctcttcttc
     1561 atgccggaga aaagggcgaa ctgacggttg cagtagatca gtccgccgga ggggctcacg
     1621 gcgaagacca tatcctgcac gctttcgatc agttcctgat agtcaaattc catggcatcg
     1681 gcctgcgctg gagtgcttcg tcacgcggtc agggtggttg tggtaaagat ctcgctttcg
     1741 ggaaattttt catcaatcat gcgcacgagg cggcggctgc tggtgaccac ggcatcgagg
     1801 tcgaggcgct gctgctcgtt cagcccctcc ggctgggcgc ggcgcagctc ggcagtgagc
     1861 cgggcgatgg tgccggcggc gatgcgcagg tcatcgctcc agcgcgcctc gggcactgcc
     1921 gctgcagggg cgctctgcgc cgttgcggtg gctgagggtg cagggcccgc cggacgggcc
     1981 ggcgaggcgg cggcaagcag aggccgcagg ctcctctcaa gacggagggc gttctccaca
     2041 acattgacca gctcacggtg gccgaagggc ttggtgagga cggcgcgcag attggcggcg
     2101 aagaggctgt caagctcctc gagatagtcg gtccgcgcgg tcaatacgat gacggggatg
     2161 gcgcgcaaag gggcataggc cggctcggcg gcgatggtct cgaggaactg cagcccgttg
     2221 aggtcgagga gcatataatc gaggatgacg atgtccacgc cccctgcagc catcctctcc
     2281 agcccctcca gtccgctgcg cgccgacagg tactcgaatc cggccttggt gaagatacgc
     2341 tgcccgatga gcagcatgtc cagattgtca tccaccatca acacagtgcc ttttttcata
     2401 atccttccct tcctcctctt tcccggcgca cgggtctaac ggtttcagcg gataatgttc
     2461 tcggcatcct cttcgttttg cgggagtccg gactgggcga tgtgggcctt gggcatataa
     2521 acggtaaata ccgagccctt gccgatctcg ctttcaacag tgatgcggcc accgtggagc
     2581 tcgatgatct gcttgacgag ggccagcccg agaccggtgc cggtactggc gcggacggcc
     2641 tcatgttcgg tgacgcggaa aaacttgtca aaaatcttgc caatggcgtt gcgcgggatg
     2701 ccatagccct gatcgtggac ctcggcaatc acctcgttct cgttctggcg cacgaggaca
     2761 tcgatacggg tgttttcggg actgtacttg accgcgttgg aaaaaaggtt gatgaaaacc
     2821 tgctccatca tgccgctgtc ggcccagatc tcggccggct catcgggggg atggacctcg
     2881 acatcgatgt tctttttggc ggccagaaaa gagttgttgc cgaccaccat ctgcacggtc
     2941 tcgaccagat cgagggcgtt tttcttgggc tggatacggc ccgattcgat gcgtgaaata
     3001 tcgaggaact tgttgatcag gtcgccgaga cggttggact ccttgaggat gatgccggcg
     3061 tactcctcct gctgttcccg gttcagctcc gggtcgagca gcagttcgct aaaacccgaa
     3121 atgctggtca ggggcgagcg cagctcatgg gcgaccatcg agaccagctc ggtcttcatg
     3181 cggtcgatct ctttctgctg ggtgatgtcg ctgaggacgg tgacgatgcc gatgagttcg
     3241 ttgtcctcct ggagaacgcg ggcggcatgg gcctggaata tgcgcggctt ccactccccc
     3301 ggaggtcgga aggtgatctc gaccggcggc agggtggtgt tgttgttcac gccggcatcg
     3361 gccaggagct ggagcatctt ctcctcctgg ataaagttct cgaggggctt ttccagggca
     3421 tccttttcgc gcaaaccgag ccagcgctcg gcagaggcat tgagaaggag caccttgccg
     3481 cggggatcgg tgaccacaac gccgtcagcg atatttttga tgatggtctg ggtcttgttg
     3541 cgttcggcga tgatctgggc cagattcatg ttgttgagac ggcgcagctc ggtggtcata
     3601 taattgaaaa gacgggcgag gcggccgatc tcgtcgttgg agagcacctt gatctcgtgg
     3661 gagaaattgc cgcgggcgat ctcggtggcg cttttagcca gatcggagat cggcaaggtg
     3721 atgttgcgcg agagaaaata ggcgacgatg ccggccagca gcagggcgag gaccacgaca
     3781 ctgaaaaagc cggggaaaag caccgcgctt ccgccgtcgg tcatccggag ccagtaaaag
     3841 cccatcagta ccagcggcag catggtggcg gcgaaggtgt aaaagaagac cttgcggaac
     3901 agtctgcttt taagctgcaa tgcgatccag ttgctcacag aagcactctc ccatcacttg
     3961 tccacgacac gattcctgcc ctccgccttg gctcggtaga gccgctggtc agcgacatgg
     4021 atcaggctgt ccgcactccg cgcatcatca ttaaaggtgg ccacgccgac gctgacagtc
     4081 aggtgaacct gactcttgcc attgctctgg ggaaagggaa aattttccac caggcggcgc
     4141 accttttccg ccacaatcct ggcctcggcc ttgttggtct cgggcaggat gatggcgaac
     4201 tcttcacccc catagcgggc cactacatcg atggcgcggg tgtggccctt catcagccgg
     4261 gcgacgatgc gcagaacctc gtcgccggca agatggccat gggcatcatt gaagcgcttg
     4321 aaaaaatcga tatccagcat cgccagcgag accgcaagct ggtgcctctc ggcgcggcgg
     4381 atttcctggg cgaggtattg gcgaaaatag cgatagttgt aaaccccggt caggccgtcg
     4441 gtgaccgcca gggcgcgcag ctgctccttc atctgctcga gctggcggat cttgacctga
     4501 agctggtcgt gcagggtctt gacgcgcaaa agcgacttga cgcgggccag cagttccagc
     4561 cggttgaagg gcttgctgat gaaatcgtcc gcaccggctt cgatgccgcg gatcttgtca
     4621 tccagctcgt tgagcgcggt gaccatgatc acgggaatgt actgggtgga tggactgtgc
     4681 ttgagatgcc ggcatgtttc aaaaccatcc atcctgggca tcatgacatc gagcaaaacc
     4741 agatcgggct gacgctccct gatccgcgcc agggcctcct cgccgttgtg ggccgacatg
     4801 gtgtcgtaac cgactgccgc aaggtagttc tggatgagct ggatgttgac cgggagatca
     4861 tcgaccacca gaataagggg tcttttgacg gtgttgagcg tttccaaagc atcgtccggg
     4921 cgactggatc gccgttcatt gggcacagag ccatcctgtg ggcgtcgctg tggaaagctc
     4981 caacctcccc tccgggagtt ccacaccacc ccgcaccacg acctctccac cccggctcac
     5041 gctgaacaac gcgcgcgggg tggaaactgg ccgaaaaccg accagttaac atacaatttc
     5101 cctgagacta tgtcaagctt tttttcacta tttggctgct ccaccccgtc cggtggcgtc
     5161 acggggttgc ggagcaaagg aggagtaaat ggcgaggcgc ggccctggcg agcctttcag
     5221 cgcccggtca gctgccgggc ccgccgccac agtccgctgc agtcgcgcac ctgggggtca
     5281 gcgagaaggg tgatctgtgt gcgcacctct tccccccaca tcatcgcagc gatctcccgc
     5341 ttgagcagca ggcggacgcc ctcacgattg cggctgagat cctgggcgga aaagttcacc
     5401 cccatcttcc tcgcttgctg ctgaaaatca cgcagcaggg attcaggggg ctcgaagttg
     5461 cggatgaagc cctcccggtc cctggccagt tcgggatggg cggcggaata agtctcgctg
     5521 aagatagcga gggggttctc ccgggcagcg agcaggcggc gcagggccgg cgagtagttg
     5581 agggagtcag cggagaggtc gacgtcgggg acgatactgc cgttggctgt cagcaggcgg
     5641 ccgccgggag tgcgcagtgt ggctccctca cggcccgtac tgcggccgcc gttctgcggc
     5701 cgcgcctggc ctgtacggaa aagagaacgg ccgagggggg tgtaaaaacg cgctgtggtc
     5761 agcagcatcg ccgaaccgtc cggaaaggtg aactcgctct gtaccagggc cttgccaaaa
     5821 ctgggcttgc cggcgatgag ggcacggtcc caatcctgca gcgccccggc gacgatctcg
     5881 gcatcactgg ccgaaccgcc gttgatgagc acgattaccg gcagggcggg cagtttgttc
     5941 tccgtgccgg cgcggaattc catgttggcc ccgggcccgc gtccggtggt ataggagatc
     6001 attttacctg cggagagaaa acggtcggcg atcttgaccc ccgcctgcag atccccgccc
     6061 atgttgtcgc gcagatcaag aatgagatag gaaggtttgg ccgcccgcag acgcgaccag
     6121 gcctggtcga actcggccgg ggtggtttgg atgaagcgct cgagcttgat gaaagcgacc
     6181 gagtcatcca gcataaaggc gctgctcacg gtttgcggca cgacgggtcg tttgctgatg
     6241 gcgaagggaa gcaggtcccg cacgccatcg cgttcgatct cgagacgcac caccggctcc
     6301 tgcagcagcg cggggagttc cgtatccgtg atatattggg tcatcttgcc gctgatgcgc
     6361 attatcttgt cgcccgggcg gatgcccgcc tctgcggccg ggccgccggg caccacggag
     6421 gcgacgagga gcttttcggg cagacgattg tatttcaggc cgatgccctg atagccgagg
     6481 aaggcgccct tgtattccgt gaattcgtcg ggggtgagat agattgaatg ggggtcgagg
     6541 gctccgagga tgccatcgat ggcccggtcg atcagttcat cggggttgcg gtcgtcaacg
     6601 taaaagcgct cgatcttgtc gagcaccatg gtcaggacca tcatcttgtt gtaggtgctc
     6661 tgcgcggcat gaatgatgcg ggccgggccg atccagagca cggcgagcac gaccaggccg
     6721 ccgaaggtaa gggcggcgat tcttttcttg cgggagtaaa gcatggctaa atttaattat
     6781 aatatcagtg agaaacaaga agcaagagaa tattcacggg accccccgaa atctggttcc
     6841 catcggagat cttgcgccgc atacccccga aatctggctc ccacccaaca ccttgcgccg
     6901 catgcccccg aaatctggct cccacccaac accttgcgcc gcatatccca gaaatctggc
     6961 tcccacccaa caccttgcgc cgcatgcccc cgaaatctgg ctcccnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnaccca acacctttcg ccgcatgccc ccgaaatctg
     7141 gctcccaccc aacaccttgc gccgcatatc cggttccctg cgtactattt ccagcctagt
     7201 cgcggcctgt gccctggcca atgaggcccc tctattcatg cgttttctcg ccagcagccc
     7261 actgtttttc tattaattac atccgggttg gacccagatt ttatgggatg ccgatgcatg
     7321 agaaaattct cttgacattt tcattgttta gaagtaattt tccaggatat aaaacaaggc
     7381 aaagccggcg atgcgggtct cacgatgagc cattt
//
LOCUS       DAOPWU010000164         7311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10154_length_7311_cov_5.200110, whole genome shotgun sequence.
ACCESSION   DAOPWU010000164 DAOPWU010000000
VERSION     DAOPWU010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10154_length_7311_cov_5.200110"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4095
                     /locus_tag="PKM23_09185"
     CDS             <1..4095
                     /locus_tag="PKM23_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNY91687.1"
                     /translation="TSGAKADIVFVFDTTGSMSGSISGVKDRASAFADALAAKGIDYR
                     LALVTYGDAVEKVNDFTADINTFKGWINGLYASGGGDTPENALEGLARGTTLTFRAVS
                     QRIFILITDANYHQAGGSGGGTTSYTTDSMIALLKSQHIMCNVVGPDYPEYRRMASET
                     GGLWYYIHGDFRGIIDKLSIVLSSQYVVTYTTHNPLRDNSWRNVVITAARGTKGGWDK
                     GRYYIAGELPNVRSFAAEAISYNRIYCRWYFPPGSLHEGVKVVRKMGGYPADVDDGEV
                     VYTGADSFFVDTGLTPETVYYYGAFTYNSSGQIGHAYSYTRDWARTWPFSSGEAGWKA
                     ETAGTTANLHAVMADTLRVIAAGDNGTVVRSSNGGASWSTGAIPAKSVVRDLFFTDPA
                     TGWLVGQNSTGAGLAMKSTDGGATWTPWPTSSAKTLLANTMVSSTVGWNVGAGGVIEK
                     TLNGGSVWTAQYNAADKSFNDVAFVNADTGWVVGDGGLIMKTLNGGAAWSVQNSGVTS
                     AIRGVNFADHAWGWAVTADGKVLRTLDGGATWSASAVSSLGLNDLSFTDRRNGIIVGE
                     GGRIYRTNDGGAAWVLETSGVTAQLNAVWLVNPWTGWVVGDNGTILSIGRSGAVLYSG
                     MTVTVNSVDAEAFPLIRSFVSVVDPESRTALTDLTAEHFRVSEEGLPQSPIKVELISS
                     GSGARADIVFVFDVTGSMGDEIAGLKARALSFADALAAKGIDFRLGLVTFGDNIEEVH
                     DFTADAAEFKAWIDGLRASGGGDVKENSLEGLARASKLSFRETSQKMAVLITDADYHQ
                     AGETGGGTTTYTTETMISLMQGRRIVTHVVGPDQSQFHTLAERTSGLWFNITADFGGI
                     IDAIGTMLTSQYVITYTTANPVPDNRWRQVAVVAERSGKSGYDIGRYYVGASRIAIAP
                     ETIIGINGESFDVTVDLQGMVNLGLVHFVIAYNAAKVDIVSWSAGDFLKQGGAGDPTL
                     LVTDDAAGLVDVSMTRIGVTSGASGSGTLMTMRFKVLTADCASDLNLNSVQLRQPDNS
                     EIPAASRGARIQAATAVGLLGDFDADMDIDLRDFTLLSGYWQPVNNTAGDIGPASGTV
                     PALTVTRDGVVNFEDLFVFTRMWNWYQSRPAARPALAKTASTLAWRITPSPEAGNLYI
                     CELMGQELSNLAMAHLSLRYNPEQLSVRTISAGSLWDEAGATTALFTGHREESGSIDV
                     SLARLAEHSTSAEVAGSGAILRLLVEERTMAGALCDWKLESLDLRDAGSYRLQALAPE
                     VSALRFSELPVRYELAQNYPNPFNSRTEVRFSVPKSGMVHIAIYNVLGQPVRTLVHEP
                     LEAGIYRRIWDGRSDTGQEVVSGLYLLRMEAGEYSEMRRMAFVK"
     gene            complement(4210..4965)
                     /locus_tag="PKM23_09190"
     CDS             complement(4210..4965)
                     /locus_tag="PKM23_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY91688.1"
                     /translation="MKKICCLLSGLLLLALPLAAQDSIAQGDAAYDRFDNAAALKHYQ
                     AAYAADSTNCIAAWKVSRAHVDIGEFAGKEAQRTNYYAGEKFARKAVALCPGDDMTHL
                     TLAIAVGRVALMEGGKKKVELSKEVKAEAEAAIKLNPRNDVAYHVLARWNREVANLSG
                     ILKAFAKVLYGGLPPASREKAAELFQKAVELNPGYVNHHFELGITYEDMGKPELARAE
                     YEKAIALPRTVFSDDVHKKEAQERLDKIIKKMK"
     gene            5422..>7311
                     /gene="uvrA"
                     /locus_tag="PKM23_09195"
     CDS             5422..>7311
                     /gene="uvrA"
                     /locus_tag="PKM23_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNY91689.1"
                     /translation="MEKEHIIIRGAREHNLKNINLEIPRNRLVVITGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGLMEKPDVDYIEGLSPAISIEQRTTGRNPRSTVGT
                     VTEIYDYLRLLFARIGVPYCYNCGKKIERQTIQQIVDQVLALPAETKIQVLAPVVRGR
                     KGEYREIFEEARRDGYVRVRLDGEVLELDQEIHPDKNKKHNIEVVVDRLIVGPRIATR
                     LTDSVETALSLASGIVLIDVVGGEELLFSEHYACIDCGISYEELAPRMFSFNTPYGAC
                     PTCGGLGTKMEIDPDLIVPDQSKPLNQGAIAPWGKMQDGWYLSLTAGVVERYGHTMET
                     PFDQLSPECRQVLLYGSDEEMEFNYRSSTGRMEGTYRNKFEGIIKNMERRYNQTESSG
                     IREWIESFMDVIPCPECRGARLRKEARAVRIGDHTITDVVGFSIDRAQHFFTHLELNT
                     RDQQIAQQILKEIRERLTFLDDVGLDYLTLDRAAGSLSGGEAQRIRLATQIGSQLVGV
                     LYILDEPSIGLHQRDNQRLIDTLMRLRDLGNSVVVVEHDKETIEKADFVVDLGPGAGE
                     HGGQVVAVGTPAEVAANPASVTGDYLAGRRVIPMPKKRRAGSGKHLLLKGASGNNLKK
                     IDVEIP"
BASE COUNT         1397 a         2320 c         2259 g         1335 t
ORIGIN      
        1 acctccgggg ccaaggctga catcgtcttt gttttcgaca ccaccggcag catgagcggc
       61 tcgatcagcg gcgtcaagga ccgggcctcg gccttcgccg acgccctcgc cgccaagggg
      121 atcgattacc gtctcgcact ggttacttac ggggacgcag tcgaaaaggt caacgatttc
      181 accgccgata tcaacacctt caaggggtgg atcaacggcc tctacgcctc gggcggcggg
      241 gataccccgg aaaatgccct cgaggggctg gcgcgcggca ccaccctcac cttccgggcg
      301 gtcagccagc gcatttttat tctcatcacc gatgcgaact atcatcaggc cggcgggagc
      361 ggcggcggta ccacctccta taccaccgat tccatgattg cactgctgaa atcccagcat
      421 attatgtgca acgtcgtcgg cccggactat cccgagtacc ggcggatggc cagtgaaacc
      481 ggcgggctct ggtactatat ccacggcgat ttccgcggca tcatcgacaa gctcagcatc
      541 gtcctctcct cccagtacgt ggtgacctac accacccaca atccgctgcg cgacaacagc
      601 tggcgcaatg tcgtcatcac cgccgcgcgt ggaaccaagg ggggatggga caagggccgc
      661 tattacatcg ccggcgagct gcccaacgtc cgcagttttg cggccgaggc gatctcctac
      721 aaccgcatct actgccgctg gtattttccg ccgggcagcc tccacgaggg ggttaaagtt
      781 gtgcgcaaga tgggaggata cccggccgac gttgatgacg gtgaggtggt ctacaccggc
      841 gccgacagct ttttcgtcga tacggggttg accccggaga cggtctatta ttacggcgcc
      901 ttcacctaca acagcagcgg ccagatcggc catgcctatt cctatacccg cgactgggcc
      961 cggacctggc ctttctcgag cggcgaggcg ggctggaaag ccgaaacagc gggcacaacc
     1021 gccaacctcc atgcggtcat ggccgacacc ttgcgcgtga tcgccgccgg cgacaacggg
     1081 accgtggtgc gcagctccaa cggcggggcg agctggagca ccggcgccat ccctgccaaa
     1141 tcggtggtgc gcgatctttt cttcaccgat ccggccacgg gatggctggt gggacagaac
     1201 agcaccggcg cgggacttgc catgaagagc accgacggcg gcgccacctg gactccctgg
     1261 ccgaccagca gcgccaaaac gctccttgcc aacaccatgg tcagcagcac ggtcggctgg
     1321 aatgtcggcg ccgggggagt gatcgaaaag acactcaacg gcggctcggt ctggaccgcc
     1381 cagtacaacg ccgcggacaa gagcttcaat gacgtcgcct ttgtcaacgc cgacaccggc
     1441 tgggtcgtcg gggacggagg cctgatcatg aagaccctca acggcggtgc cgcctggagt
     1501 gtgcagaaca gcggggtcac cagcgccatc cgcggggtga actttgccga tcacgcatgg
     1561 ggctgggcgg tgactgccga cggcaaggtg ctgcggacac tcgacggcgg cgccacctgg
     1621 agcgcctctg cagtcagctc cctcggcctc aacgatctca gcttcaccga ccgccgcaac
     1681 ggcatcatcg tcggggaggg cgggcgcatc taccgcacca atgacggcgg ggccgcctgg
     1741 gttctggaga cgagcggcgt gaccgcacag ctcaatgcgg tctggctggt caatccctgg
     1801 accggctggg tggtcggcga caacggcacc atcctcagca tcggccgcag cggcgccgta
     1861 ctctacagcg ggatgacggt gaccgtcaac agcgtcgatg ccgaggcttt ccccctcatc
     1921 cgcagcttcg tctcggtggt cgatccggag agccgcacgg ccctcaccga tctgaccgcc
     1981 gaacatttca gggtgagcga ggaggggctg ccgcaatcac cgatcaaggt ggagctgatc
     2041 agcagcggtt cgggtgcccg tgcggatatc gtcttcgtct tcgatgtcac cggcagcatg
     2101 ggcgacgaga tcgccggact caaggcacgc gccctcagct tcgccgacgc ccttgccgcc
     2161 aaggggatcg atttccgcct cggcctggtc accttcgggg ataacatcga ggaggtgcat
     2221 gatttcaccg ccgacgcggc cgagttcaag gcctggatcg acgggctgcg cgcctccggc
     2281 ggtggcgacg tcaaggagaa ctccctcgag ggactggcgc gagccagcaa gctctccttc
     2341 cgcgagacca gtcagaagat ggcggtgctg atcaccgatg ccgattatca tcaggccggc
     2401 gagaccggcg gaggcaccac cacctacacg accgagacca tgatctccct catgcaggga
     2461 cgccggattg tcacccatgt ggtcggtccg gatcagtccc aattccatac cctggcggag
     2521 cggaccagcg gcctctggtt caacatcacc gctgatttcg gcggcatcat cgacgccatc
     2581 ggcaccatgt tgacctcgca gtacgtcatc acctatacca ccgccaatcc ggtgcccgac
     2641 aaccggtggc gccaggtggc cgtcgtggcc gagcgcagcg gcaagagcgg ctatgacatc
     2701 ggccgctatt atgtgggcgc cagccgcatc gccatcgctc cggagaccat catcggcatc
     2761 aacggcgaga gcttcgatgt gaccgtcgat cttcagggca tggtcaatct cggtctggtc
     2821 cattttgtca tcgcctacaa cgccgccaag gtcgatatcg tgagctggag tgcgggtgac
     2881 tttttgaagc aggggggggc aggcgatccg acccttctgg tcaccgacga cgccgccgga
     2941 ctggtcgatg tcagtatgac ccgcatcggg gtcaccagcg gcgccagcgg cagcggcacc
     3001 ctcatgacca tgcgcttcaa ggtcctcact gccgactgcg ccagcgacct gaatctcaac
     3061 tccgtgcagc tgcggcagcc ggataacagc gagatcccgg ccgcgagcag gggcgcccgc
     3121 atccaggcgg cgaccgccgt cgggctgctc ggcgatttcg acgctgatat ggatatcgat
     3181 ctgcgcgact ttaccctgct ttcgggctac tggcagccgg tgaacaacac cgccggcgat
     3241 atcggaccgg ccagcggcac cgtgccggcc ttgaccgtga cccgggacgg cgtggtcaac
     3301 tttgaggatc tcttcgtctt cacgcggatg tggaactggt accagtcccg tccggccgca
     3361 cgccccgccc tcgccaaaac ggcttccacc ctggcctggc ggatcacccc ctcgccggag
     3421 gccggcaacc tctacatctg cgaactgatg gggcaggaac tttccaacct ggccatggcc
     3481 catctcagcc tgcggtataa tcccgaacag ctgagcgtcc gcacgatttc cgcgggctcc
     3541 ctgtgggatg aggcaggtgc aaccaccgcc ctcttcaccg gtcaccgcga ggagagcggg
     3601 agcatcgatg tcagcctggc ccgtctggcc gaacacagta cttcggccga ggtcgcgggc
     3661 tccggggcga tcctgcgtct gctcgtcgag gagcgcacca tggccggcgc cctctgcgac
     3721 tggaaactgg agagcctcga tctgcgcgat gccggcagct accgcctgca ggccctggcg
     3781 ccggaggttt cagccctgcg cttcagcgag ctgcctgtcc gctatgagct cgcacagaac
     3841 tatcccaatc ccttcaacag ccgcaccgag gtgcgtttct cggtgccgaa gagcgggatg
     3901 gtgcacatcg ccatctacaa cgtcctcggc cagccggtgc gcaccctggt ccatgaaccc
     3961 ctcgaggcgg gtatctaccg ccggatctgg gacggccgca gcgataccgg tcaggaggtg
     4021 gtgagcggac tctacctgct gcgcatggag gcgggtgaat acagcgaaat gcggcggatg
     4081 gccttcgtca agtagccttc cccgagtgag cgcccatttc ggcgcgctcc aaacaaaaag
     4141 gcctcccgga tcatggtccg ggaggccttt ttcatgttta tgacggtgcg ccaggcggcg
     4201 cccaggcttt tacttcatct ttttaatgat cttgtccaga cgctcctgcg cctctttctt
     4261 gtggacatcg tcgctgaaaa cggtcctggg cagggcgatc gccttttcat actccgcccg
     4321 ggcgagttcc ggcttgccca tgtcttcata ggtgatgccg agctcgaagt ggtggttgac
     4381 gtagccggga ttgagttcga ccgccttttg gaagagttcg gctgcctttt cgcggctggc
     4441 cggggggagt ccaccgtaga ggaccttggc gaaggccttg aggatgccgc tgaggttggc
     4501 gacctcgcgg ttccagcggg cgaggacgtg ataggcgaca tcattgcggg gattgagctt
     4561 gatggcggcc tcggcctcgg ccttgacctc cttggagagc tcgacctttt tcttgccgcc
     4621 ctccatgagg gcgacgcggc cgacggcgat ggcgagggtg agatgggtca tgtcgtcacc
     4681 ggggcagagg gccacggcct tgcgggcgaa tttctcgccg gcgtaataat tggtgcgctg
     4741 ggcttctttg ccggcgaatt cgccgatgtc gacatgggcg cgcgagacct tccaggcggc
     4801 gatgcagttg gtcgagtcgg cggcgtaggc ggcctggtag tgtttcaggg ccgcggcgtt
     4861 gtcaaagcgg tcgtaggcgg catcgccctg ggcgatggaa tcctgggcgg ccagcggcag
     4921 ggccagcaga agcaggccgg aaagcaggca gcagattttt ttcatgttga tctccggatg
     4981 tcagggtaaa attactgaat tgtattcatt attttactgc ttttttgcca caatgtcaat
     5041 ctttttctct cccctttgcc tgcagtgtac aaaactggct gccccccaaa ctctcggtcc
     5101 cacccaaaga taattaaata aaaaatagta ttttacctgc cagaaacacc ttgtataata
     5161 attgcccgtg acgagggcag aggccgagag catgctggga atggcacaga aggagtgggg
     5221 aacgcggcac aaggcgtcag gtgggagcca gatttcgggg gaacgcggca cacggtgtca
     5281 ggtgggagcc agatttcggg ggaacgcggc acacggtgtc aggtaggatc cagatttcgt
     5341 ggataccctg tacacaaaat gctttaatat tggtcattgt ttttgtacat ttatggatac
     5401 gtatcaggga aaagcaggcc tatggaaaaa gagcatatca tcattcgcgg cgcccgcgaa
     5461 cacaatctca agaatatcaa tctcgaaatc ccgcgcaacc ggctggtcgt gatcaccggc
     5521 ctctcgggga gcggcaaatc ctccctcgcc ttcgacaccc tttatgccga gggacagcgc
     5581 cgctacgtcg agtccctctc cgcctacgcc cggcagtttc tcgggctgat ggaaaaaccg
     5641 gatgttgatt acatcgaggg gctctcgccg gcgatctcga tcgagcagcg cacgaccggc
     5701 cgcaaccccc gttcgacagt aggcaccgtg accgagatct atgactatct gcgcctgctc
     5761 ttcgcccgca tcggggtgcc ctactgctac aactgcggaa aaaagattga acgccagacc
     5821 atccagcaga tcgtcgacca ggtcctggcc ctgccggccg agaccaaaat ccaggtcctc
     5881 gcgccggtgg tgcgcggccg caagggtgag taccgcgaaa tctttgaaga agcgcgccgc
     5941 gacggctatg tgcgcgtccg cctcgatggc gaggtcctcg agcttgacca ggagatccat
     6001 cccgacaaga acaagaagca caatatcgag gtggtggtcg accgcctcat cgtcggtccg
     6061 aggatagcca cgcggctgac cgattcggtc gagaccgccc tcagccttgc ttcgggcatc
     6121 gtcctgatcg atgtcgtcgg cggcgaagag ctgctcttca gcgagcatta tgcctgcatc
     6181 gactgcggca tctcctatga ggagctggcc ccgcgcatgt tctccttcaa caccccctat
     6241 ggcgcctgtc cgacctgcgg cggcctcggc accaaaatgg agatcgatcc cgacctgatc
     6301 gtgcccgacc agtcaaaacc cctcaatcag ggcgccatcg ccccctgggg caagatgcag
     6361 gacggctggt atctcagcct caccgccggg gtggtggagc gttacggcca taccatggag
     6421 acccccttcg accagctctc cccggaatgc cggcaggtgc tgctgtacgg ctccgatgag
     6481 gagatggagt tcaactaccg ctcctcgacc ggccgcatgg agggcaccta ccgcaacaag
     6541 tttgaaggca tcatcaaaaa tatggagcgg cgctacaacc agaccgagtc aagcggcatc
     6601 cgcgaatgga tcgaaagctt catggacgtc atcccctgtc ccgagtgccg gggggcgcgg
     6661 ctgcgcaaag aggcacgcgc ggtgcggatc ggcgaccaca ccattaccga tgtggtggga
     6721 ttctccatcg accgggcgca gcactttttc acccatctcg agctaaacac ccgcgaccag
     6781 cagatcgccc agcagatcct caaggagatc cgcgagcggc tcacctttct cgacgatgtc
     6841 gggcttgact atctcaccct cgaccgcgct gcggggagcc tgtcgggagg agaggcccag
     6901 cgcatccgcc tggccaccca gatcggctcg cagctggtgg gggtgctcta tatcctcgat
     6961 gagccctcga tcggcctgca tcagcgcgac aaccagcgcc tcatcgacac cctgatgcgg
     7021 ctgcgggacc tcggcaattc ggtggtggtg gtcgagcatg acaaggagac cattgagaaa
     7081 gccgatttcg tcgtcgatct cggccccggc gccggcgaac acggcggcca ggtggtggcc
     7141 gtgggaacgc cggcggaggt ggcggccaat ccagcctccg tcaccgggga ctatctggcc
     7201 ggtcggcgcg tcatccccat gccgaaaaaa cggcgggcgg gaagcggcaa gcatttgctt
     7261 cttaaagggg cgagcggcaa taatcttaaa aagatcgatg tcgaaatccc c
//
LOCUS       DAOPWU010000165         7307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10168_length_7307_cov_4.511859, whole genome shotgun sequence.
ACCESSION   DAOPWU010000165 DAOPWU010000000
VERSION     DAOPWU010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10168_length_7307_cov_4.511859"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3144
                     /locus_tag="PKM23_09200"
     CDS             <1..3144
                     /locus_tag="PKM23_09200"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNY91690.1"
                     /translation="TWAHPWLVPHPLLYYLIRFVIAFMVMLPATFFMGGTLPVMSRAF
                     IRGRQRGAGQLGWLYGINTLGGMAGCLATAFFLLRFSGFTAAYAAAMAIDVLIACAAW
                     LLARSSGESAINAPGRSRISLSAAERVILTAMALSGFIAMSYEVLWSRLLVFILTNAT
                     YSFSIMLAAFLASIALGGFAGGRWAERTANPRRLLGLLEIGAGLCAALAFLLLLYLPQ
                     LQHHFFMPTAMTSWWEWNGIRFLQAFLVTSLAGLFLGATFPAGYRALSDGPEQTGTKV
                     GELYFYNTLGGILGSLATGLLLIGWLGAAAAMLVMIVLNILLGAWLLSGRLLRQSLIL
                     AIFALILAAAITRLTPPALLTRLYSISETEYPLVAMREGIEGTVTVHRHASGDTGERR
                     IDVDGLNVAGSSRMLRTLQLLQGHLPMMLHSDPQKVLQIGFGTGQTSWAALSHPISSF
                     QLVEISPDVLSLSSEFFQDLNRDVMKDPRFQPLILDGKNQLRYTGERYDVIMNDANYA
                     VATASASLFTRDHFMNGRRRLNPGGIFSTWMTTDLHPEDFRIVLKTFQSVFPHAVLWM
                     APNCINKQVVLMGSVDPLKIDWAKWQERWPQAEAGLESVDIRSPHDLFACLLLDEQGM
                     ARLSAGAALNSDDHPVLEFSTRDVRARDLCAFQNLSAMLAEPADWRQYLRLDANRSGL
                     EAGAMAQIERYARASRQMFQGMLQFYQGQTRRALSTMLNSSRQIPESRLASGFYEEVD
                     RQTLILVQTINRAPAAIAPRLEVIRHYIGLERYEEAAHLLRSLLRVDVQQPAAAYEMA
                     RIHFLQARIDSARIWIDRAVNEGGKSAGASFLSGRIAERENALDRALGEYRSALARDP
                     GMLEAHARIGHILLQQGRYAEARQSLLASLQAAPAQGAVAMEMGDAFLFEEKPEQAAA
                     WYRRAQLLGQTGPHLYHNLGNALALLGIPGDAVECYRSALRQDPDNAELHYNLAGVLI
                     RLDQDREAVQLLERAISLDPGQSDYFNNLAMLHRKRGDYAAALRIFDEGLRRHPASRL
                     LLTNRQTTLEENTRRLK"
     gene            3141..3749
                     /locus_tag="PKM23_09205"
     CDS             3141..3749
                     /locus_tag="PKM23_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91691.1"
                     /translation="MRKALWIGCAALLIIGCAGTEKMMLQPPQPDKSLVVGAVLVENL
                     GLEEVYEAKTARIWVVLVGKGVENGVEKIEGYRCRTDENGYFMLQNVPRGTYIIKGIE
                     VDLGFSIHMILNSRWEGNTQIFEPGGTMIDHIVRVWPEMAPERVVDLGIRYFRFDYSG
                     RIQDQTFAQLRGNSLGLKDKVYTMPSPAAYFSQKNPEWGWFK"
     gene            3845..5521
                     /locus_tag="PKM23_09210"
     CDS             3845..5521
                     /locus_tag="PKM23_09210"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNY91692.1"
                     /translation="MKRYIFALLLILPVAVPAQEPALRRIDFTQSAEVFANPERGFYR
                     YANLPNLSSLPDLQKEAVSLIFGRVAANAYRDKDFTQSYLSQIQRGFDTARARGVKVN
                     LRVSYSDNIGQADASREQIFRHIDQLQPLLAKNKDVINLVEAGFIGAWGEWHSSTHGL
                     ETPENRRDILFKLLSALPADRMVVVRTPHFKRQIFRDSVITAGRVFDGSYLSRTGFHN
                     DCFLSGPDDMGTYIERSRAKEIEYIGGETRFTPFGGETCGVSSYAECRNAVAEMEKLH
                     CSYLNDGYHPDVLALWKSSGCLSEVKKRLGYRFVLRGAELSEAVRPGGVLVFNFMIEN
                     VGFAAPHNPRALCLVLRDAASGREAAAAISLDPRTWLPGDTLSFSRRFRLPGDWPAGA
                     TSVALWLPDPEPSLRDDPRYAIRLANSGIWEQNRGMNRITDQLLIDPGAQGQVDMQAR
                     ELVEIDSRAEVGSRPAIPAVYELAAFPNPFNSQVTIQYRLSRLEVVHLEICNLQGRSL
                     CILADGPQNTGRYTVQWDGRSAGRPAASGVYLIRLRTRSSEEIRRIVLAR"
     gene            5569..>7307
                     /locus_tag="PKM23_09215"
     CDS             5569..>7307
                     /locus_tag="PKM23_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNY91693.1"
                     /translation="MQYKKTAALVALLAMTALVLFCTGAPESVTITHKKIQIRYDHLL
                     HSEVIALSGASTAIGGISASEYPVIDGVAVTDFELKTHNIAGVRDALGQGKQHILVGQ
                     APGLEKKLTVTLYEDFPAAAVVQVTYTNTGAKTVQLDTWTNHHYSVKAGPAVSGQAPF
                     WSYEPATYESRPDWFLPVKPGFSQENFLGMNASDYGGGTPVADLWRPDIGLGIGHLEL
                     VPKLVSLPVRMADSLAAELSIQYKADKPLPPGESYSTFTTFVMVHQGDCFTTLAAYRE
                     MMIRRGIPANKYPDTAYEPQWCAWGYERKFTMDQVVNTLPKAKELGYQWAVLDDGWQT
                     AEGDWYLTKDKFPNGDADMIAFVDRIKAGGLKPQLWWCPLAVDPGTDLLRDHPEYLLL
                     NKDGSKQDISWWDSYYLCPAYAPVVEFTRSQVVQIMQKWGYQGLKIDGQHLNAVPPCY
                     NPAHHHAYPEESCEKLPDFFKMIYETALGIYPEAVVEICPCGTSYAFHTMPYMNQSVA
                     SDPTSSWQTRLKGKALKALMGENAAYYGDHVELTDGGDDFASQVGVGAVIGTKFTWPE
                     GAAPGRETTSPRRG"
BASE COUNT         1374 a         2277 c         2150 g         1506 t
ORIGIN      
        1 acctgggccc atccgtggct ggtgccgcat cccttgctct actatctgat cagatttgtc
       61 attgctttca tggtgatgct gccggccacc tttttcatgg gcggcaccct gccggtgatg
      121 agcagggctt tcatccgcgg ccggcagcgg ggcgcagggc agctgggatg gctctacggc
      181 atcaataccc tgggcgggat ggccggatgc ctggccacgg cctttttctt gcttcgcttt
      241 tcgggtttta cagccgctta tgcggcggcg atggccatcg atgttctcat cgcatgcgca
      301 gcatggttgc tcgcccgcag ttcgggggag tcagccatca acgcacccgg ccgctcgcgg
      361 atttccctga gtgcggccga gagggtgatc ctgaccgcca tggctctctc cggctttatc
      421 gccatgtctt acgaggtgct ttggagccgc ctcctggttt ttatcctcac caacgccacc
      481 tactcctttt ccatcatgct ggccgccttt ctggccagta tcgccttggg cggcttcgcc
      541 ggcggtcgct gggcggagcg caccgccaac ccccggcgct tgctgggcct gcttgagatc
      601 ggggcgggcc tatgcgcggc actggccttc ctgcttcttc tgtaccttcc gcagctgcag
      661 caccatttct tcatgcccac ggccatgacc tcctggtggg aatggaacgg catccgtttt
      721 ctgcaagcct ttctcgtcac cagcctggcg ggcttgttcc tcggcgccac ctttcctgcc
      781 ggatacagag cgctctcgga cggcccggaa cagaccggaa ccaaagtggg tgaactctac
      841 ttctataaca ccctaggcgg cattctgggt tcgctagcaa ccggactgct gctcatcggc
      901 tggttggggg ccgccgccgc gatgctggtg atgatcgtgc tcaatatcct cctcggcgcc
      961 tggctgctga gcggccggct gctgcggcag tcccttattc tcgcgatatt tgccctgata
     1021 ctggccgcag cgatcacccg actgacgccg ccggccctgc tgacgcggct ctattccatc
     1081 tccgagacgg agtatcccct tgtggcgatg cgtgaaggca tcgagggcac cgtgacagtg
     1141 caccggcatg ccagcggcga tacaggggag aggcgcatcg acgtggacgg cctgaatgtg
     1201 gcgggcagca gccgtatgct gcgcactttg caactgctcc agggccatct gcccatgatg
     1261 cttcattcgg atccgcaaaa ggtactgcag atcggttttg gcaccggcca gacctcgtgg
     1321 gccgccctgt cacatcccat ctcttcgttc cagcttgtcg agatctcgcc ggatgtgctc
     1381 agtctctcct cggagttttt ccaggacctg aaccgggatg tgatgaagga tccacgcttc
     1441 cagccgctca tccttgacgg caaaaaccag ctccgctaca ccggcgagcg ctacgacgtc
     1501 atcatgaacg atgccaacta tgcggttgcg acggccagcg ccagcctgtt tacgcgcgac
     1561 cactttatga acggccgtcg caggctcaat cccggcggca ttttctcgac ctggatgacg
     1621 acggatctcc atcccgagga ttttcgcatc gttctcaaaa catttcagtc ggtttttccc
     1681 cacgccgtgc tgtggatggc ccccaactgc atcaacaaac aggtggtgct gatgggttct
     1741 gtggatcccc tcaagatcga ttgggcgaaa tggcaggaga gatggccgca agcggaagca
     1801 ggactggaga gtgtggatat ccgttctccg catgatttat tcgcttgtct gctcctggac
     1861 gagcagggca tggccaggtt gagcgcgggt gccgccctca acagcgatga ccatcctgtg
     1921 ctggaattct cgacccgtga tgtccgcgcc cgcgacctct gcgcctttca gaatctctcc
     1981 gccatgctgg ccgaacctgc agactggcgg cagtatctcc ggttggacgc caaccgttcc
     2041 ggcctggagg cgggggcgat ggcgcagatc gaacgctatg cgcgggcttc ccggcagatg
     2101 ttccagggca tgctccagtt ttatcaggga cagaccagac gggctctctc caccatgctg
     2161 aacagctcgc gccagattcc ggagagccgc cttgcctccg gcttttacga agaggtggat
     2221 cgccagaccc tgattctggt gcagaccata aaccgggccc cggcagctat tgctccccgg
     2281 ctggaagtga tacggcatta catcgggttg gagcgttatg aggaggctgc acatctcctg
     2341 cgttctttgc tccgcgttga tgtccagcag ccggcggcgg cctatgagat ggcgcggatc
     2401 cattttttac aagctcggat cgattccgcc cgtatctgga ttgatcgcgc cgtgaatgag
     2461 ggcggaaaga gcgccggcgc cagttttctg tccgggcgca tcgccgagag ggagaatgcg
     2521 ctcgaccgcg ccctgggtga ataccgcagc gctctcgccc gcgatcccgg catgctcgag
     2581 gcccatgcgc gcattggtca catcctgctg cagcagggcc gctacgcgga agcgcggcag
     2641 agcctgctcg caagtctgca ggccgcgccg gcacagggag cggtggcgat ggagatgggc
     2701 gacgcctttc tttttgaaga aaagccggag caggcggcag cctggtacag acgggcccag
     2761 ctcctcggcc aaaccgggcc gcacctctat cataatctgg gcaatgccct cgcgctgctc
     2821 ggcattcctg gtgatgcagt cgaatgttac cggagtgcgc tgcggcagga tcccgacaat
     2881 gcagaactcc attacaatct tgccggggtt ttgatccggc tggatcagga tcgggaggcg
     2941 gtgcagctgc tggaacgcgc catcagcctg gatcccggtc agtccgatta ttttaacaat
     3001 ctggccatgc tccaccggaa gagaggcgac tatgccgccg ccttgcggat ctttgatgag
     3061 gggctgcgcc gtcaccccgc ctcccggttg ctgctaacga atcggcagac gacgctcgaa
     3121 gagaatacac ggaggttaaa atgagaaaag cattatggat aggttgtgcg gctctgctga
     3181 tcatcggctg cgcgggcacg gaaaagatga tgctgcagcc gccgcagccg gacaagagcc
     3241 tggttgtcgg ggccgtcctg gtggaaaatc ttggcctgga agaggtctat gaggccaaaa
     3301 ctgcccggat ctgggtcgtg ctggtgggca agggcgtcga gaacggggtg gaaaagatcg
     3361 agggctaccg ctgccgcacc gacgagaacg gctatttcat gctgcagaat gtgccgcgcg
     3421 gaacctatat catcaagggc atcgaggtcg atctcggctt ttcaatccac atgatcctca
     3481 attcgcgctg ggaaggcaat acccagattt tcgagccggg cggaaccatg attgatcaca
     3541 tcgtccgtgt ctggccggag atggccccgg aacgggttgt cgatctgggc atccgctact
     3601 tccgctttga ttattccggc cggatccagg atcaaacctt tgcccagctg cgcggaaaca
     3661 gcctgggttt gaaggacaag gtctacacca tgccgtcgcc ggcagcctat ttcagtcaga
     3721 aaaatccgga atggggatgg tttaaatgag gtgacaaggg aacgacgtac cttagcgcat
     3781 cccgggcgcc gtccgactgc agcggtgccc gggatgcgcc atccttaaac ttgaaaggta
     3841 gtacgtgaaa aggtatattt ttgccctgct gcttatcttg cccgtcgctg ttcctgcgca
     3901 ggagccggcg ctgaggcgaa tcgattttac tcaatccgcg gaggtctttg ccaacccgga
     3961 gcggggcttt taccgctatg ccaatctgcc caatctgagc tcgctgcccg atctgcagaa
     4021 ggaagctgtc agcctgatct tcgggcgtgt cgccgccaac gcttaccgcg acaaggattt
     4081 cactcagagc tatctctccc agatccagcg cggtttcgat accgcccgcg cccgaggcgt
     4141 caaggtaaat ctgcgggtct cttacagcga caatatcggc caggcggatg cctcgcggga
     4201 gcaaatcttc cgccacatcg atcagcttca gccattgctg gcaaagaaca aggatgtcat
     4261 caatctggtc gaggccggat tcatcggcgc ctggggcgaa tggcacagct ccacccacgg
     4321 cctcgagaca cccgagaacc ggcgggatat cctcttcaag ctgctttccg ccctgcccgc
     4381 tgaccgcatg gtcgtggtac gcacccccca tttcaaacgg cagatcttcc gggattcggt
     4441 gatcactgcc ggccgcgtct ttgacggcag ctatctgagc aggaccggtt ttcacaacga
     4501 ctgttttctg tcgggaccgg atgatatggg cacctacatc gagcggtcgc gggcaaagga
     4561 gatcgagtat atcggcggcg aaacacgctt tacccccttc ggtggagaga cctgcggtgt
     4621 ttccagctat gcggaatgcc ggaatgccgt tgcagagatg gaaaaactcc attgttccta
     4681 tctcaatgac ggctatcatc cggatgtcct tgcactctgg aaaagttccg gctgcctgtc
     4741 cgaagttaaa aagcgcctgg gctatcgctt tgtgctgcgc ggagcggagc tgtcggaagc
     4801 ggtgcgtccg ggcggagtgc tggtttttaa tttcatgatc gaaaatgtgg gcttcgccgc
     4861 accgcataat ccgcgcgcgc tctgcctggt gctgcgggat gctgcctccg gccgcgaggc
     4921 cgcggccgcg atctcgctgg atccgcgcac ctggctgccg ggcgacaccc tctccttttc
     4981 acgccggttt cgccttcccg gggattggcc cgccggtgct acttcagttg cgctgtggct
     5041 gcccgatccg gaaccctcac tgcgggatga tccacgctat gccatccgcc tcgccaacag
     5101 cggcatctgg gagcagaacc ggggcatgaa ccggatcacc gatcagcttt taatcgatcc
     5161 cggcgcccag ggccaggtcg atatgcaggc ccgtgaactg gtcgagatcg attcccgggc
     5221 cgaggttggc agccgcccgg cgattcccgc ggtgtatgaa ctcgccgcgt ttcccaatcc
     5281 cttcaacagc caagtcacca tccagtatcg tctctccagg ctggaggttg tgcatctcga
     5341 gatctgcaat ctgcagggcc gcagcttgtg catcctggcc gatggtccac agaacaccgg
     5401 ccgctatacc gtgcagtggg acggacgcag cgccggccgc ccggccgctt ccggcgtcta
     5461 tctcatccgg ctgcgaaccc gttccagtga agagattaga agaatagtac tggccagata
     5521 gattctggcc ttcaccctga atccacagca acttccggag gaattgacat gcaatacaag
     5581 aaaacagctg cacttgtggc cttgctggcc atgaccgctc tcgtcctttt ctgcaccggt
     5641 gcgccggaga gtgtgaccat cactcacaag aagatccaga tccgctatga tcacctgctg
     5701 cacagcgagg tgatcgctct gtccggcgca tccactgcca tcggcgggat ctccgcttcg
     5761 gaatatccgg tcatcgacgg cgtggcggtg accgatttcg agcttaaaac ccataatatc
     5821 gcaggggtcc gcgacgccct cggccaaggc aaacagcaca tccttgtcgg tcaggcgccc
     5881 ggcctggaga aaaagctcac cgtcaccctg tacgaggatt ttccggcggc cgccgtggtg
     5941 caggtgacct ataccaacac cggtgccaag accgtccagc tcgacacctg gaccaaccat
     6001 cattactccg tcaaggcggg gccggctgtc agcggacaag cgcccttctg gagctatgaa
     6061 ccggccacct atgaatcccg ccccgactgg ttcctgccgg tcaagcccgg attcagccag
     6121 gagaatttcc tcggcatgaa tgcctccgac tatggcggcg gcacgccggt cgccgatctc
     6181 tggcggccgg acatcggtct cggcatcggc cacctcgaac tggtgcccaa actggtctcc
     6241 ctgccggtgc gaatggcgga cagcctcgcc gccgagctga gcattcagta caaagcggac
     6301 aagcctctcc ctccgggtga gagctattcc acctttacga ctttcgtcat ggtccaccag
     6361 ggcgactgtt tcacgaccct ggccgcctat cgcgagatga tgatccgccg cggcatccct
     6421 gccaacaaat acccggatac cgcctatgag ccgcagtggt gtgcctgggg ttatgagcgc
     6481 aagttcacca tggaccaggt cgtcaatacc ctgcccaagg ccaaggaact cggctatcag
     6541 tgggcggtgc tcgacgacgg ctggcagacc gccgagggcg actggtacct gaccaaagac
     6601 aaattcccca acggtgatgc cgacatgatc gcctttgtcg accgcatcaa ggccggcgga
     6661 ctcaaaccgc agctctggtg gtgcccactg gccgtcgacc ctggcaccga tttgcttaga
     6721 gaccacccgg aatacctgct gctcaacaag gacggctcca agcaggacat cagctggtgg
     6781 gactcttatt atctctgtcc cgcctacgcc ccggtggtgg aattcacccg cagccaggtg
     6841 gtgcagatca tgcaaaaatg gggctatcag gggctgaaga tcgacggcca gcatctcaac
     6901 gccgttccgc cctgctacaa tccggcccac catcacgcct atcccgaaga gtcctgcgaa
     6961 aagctgcccg actttttcaa gatgatctac gagaccgccc tgggcatcta tcccgaagcg
     7021 gtggtggaga tctgtccctg cgggacctct tacgccttcc acaccatgcc ctacatgaac
     7081 cagtcggtgg cttcagatcc caccagctcg tggcaaaccc gcctcaaagg caaggccctc
     7141 aaggcgctga tgggggaaaa tgcggcttat tatggcgacc atgtcgaatt gaccgatggc
     7201 ggggatgatt ttgcctccca ggttggtgtc ggcgccgtga tcggcaccaa gttcacctgg
     7261 ccggaaggag cggcccccgg ccgagaaacg acctcaccca ggcgcgg
//
LOCUS       DAOPWU010000166         7200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10379_length_7200_cov_5.044507, whole genome shotgun sequence.
ACCESSION   DAOPWU010000166 DAOPWU010000000
VERSION     DAOPWU010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10379_length_7200_cov_5.044507"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKM23_09220"
     CDS             complement(<1..415)
                     /locus_tag="PKM23_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HNY91694.1"
                     /translation="MSDSPKKNPKPGAARKLPVKAYANEHFLNSSDARTIRILSEYLE
                     PQMRLRRRHIRNTVVFYGSARVLPLDVAQKQLKSVLSQSQRNGTAGADFQREFDKASH
                     AVRLAQYYEAARALAHRLTEWSLGLSARDRFIVCSG"
     gene            complement(438..1190)
                     /locus_tag="PKM23_09225"
     CDS             complement(438..1190)
                     /locus_tag="PKM23_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNY91695.1"
                     /translation="MPQPLPELPVTFLSQGHQIVAMHHKSRGERLVILCHGFMSSKAE
                     CRRLFVETGRAFAAEGLDVLRFDFYGSGDSAGDFGDSLISLYIANLCDAIAWARGLGF
                     ARIAVLGHSMGGATAILTLAETPVDALVTWSAVPDMQATFQSYISNLDAISAQAEEYI
                     HEGWVIRRSFWEDAIRYDVRAALGALTMPKLCVQGMADAPVFIQGFDSFRDIVQPPAD
                     FMELPAASHTYALPAHRHQAIRQSLIWLKRNL"
     gene            complement(1533..3152)
                     /gene="groL"
                     /locus_tag="PKM23_09230"
     CDS             complement(1533..3152)
                     /gene="groL"
                     /locus_tag="PKM23_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857353.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNY91696.1"
                     /translation="MAKIIEFNVDARNAMKVGVDKLAKAVGSTLGPKGRNVVIDKKFG
                     APTVTKDGVTVAKEVELEDPLENMGAQMVREVASKTSDVAGDGTTTATVLAQAIVREG
                     LKNVTAGSNPMALKRGIEKGVKSVIEELRKQSKPIPGKTEIAQVGAISANNDKAIGEL
                     IADAMEKVGKDGVITVEEAKSTDTTLEVVEGMQFDRGYISPYFVTNSDTMEAVLEEVM
                     VLIYDKKISAMKDLLPILEKVAQTGRPMLIIAEDIEGEALATLVVNKLRGTLRVAAVK
                     APGFGDRRKAMLEDIAVLTGGRVISEEAGFKLENTTMSDLGKAKRITIDKDNTTIVEG
                     AGKTEDIKARISQIKKQIESSTSDYDKEKLQERLAKLAGGVAVLQVGAATEVEMKEKK
                     ARVEDALHATRAAVEEGIVPGGGIAYLRALPALDKIQVNGDEKIGVAILRRALEEPIR
                     LIAENAGWEGSIVVQKVRDTDGAFGFNAESETFEDLYKAGVIDPTKVSRIALENAASV
                     ASLFLMTECAIAEKPEPKPATPPMPPGADMY"
     gene            complement(3201..3485)
                     /gene="groES"
                     /locus_tag="PKM23_09235"
     CDS             complement(3201..3485)
                     /gene="groES"
                     /locus_tag="PKM23_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNY91697.1"
                     /translation="MTIKPLADRVVVKPVEEQEMKQGSIIIPDTAKEKPMQGTIVAVG
                     PGKISENGAKIEMSVKVGDRILYGKYSGTEVTIDKEDYLIMRESDIFAII"
     gene            3821..4051
                     /locus_tag="PKM23_09240"
     CDS             3821..4051
                     /locus_tag="PKM23_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNY91698.1"
                     /translation="MKEFVEYIVKHLVDNPDEVKVSEVFGETTVVYELRVSQPDMGKV
                     IGKNGRTAHAIRVLLSAVSRKAGKHATLEILE"
     gene            complement(4175..4639)
                     /locus_tag="PKM23_09245"
     CDS             complement(4175..4639)
                     /locus_tag="PKM23_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNY91699.1"
                     /translation="MKIRILTVGKPSDPHYNALCEGYLGRIRHYLPVEHHFIRQVRAT
                     GAVAEILAREEAQLLEKIGAQEWCVVLESRGEGMDSLAFAAALERHMVGGRKSLLFIV
                     GGPHGLGDGVLRRANQRLSLSAMTMAHELALTVLLEQLFRALTILRGEGYHK"
     gene            4758..4979
                     /locus_tag="PKM23_09250"
     CDS             4758..4979
                     /locus_tag="PKM23_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91700.1"
                     /translation="MADDDKNAGVTSSRIKFCDLRCEHASFPSAEHLDGANSCRTFAA
                     LWCRELQQVVTKNAPCALLFGARRPKSNL"
     gene            complement(5010..6323)
                     /locus_tag="PKM23_09255"
     CDS             complement(5010..6323)
                     /locus_tag="PKM23_09255"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNY91701.1"
                     /translation="MKKEILHKVRYPLLLAAAAILAVWLWTARKSDRPVEQAELKPRE
                     KIYLRREGTFRPGETLEKVLLRDIYDRVLADELIQGFARVFDVRKIRGDRRYTILTDS
                     AGVVHGFEYYPDAVKTVRVLRDSTGVLQPELVQVPLIKRTLALTGEVRTTLYDAVKAM
                     GESDELIAAFSDLFQWDIDFFVDPRVGDEFRMVYEAEYALDPSRPDSLGEFIRYGRVL
                     SGQYRSGSAVRTALYFEQGERGGYYDLEGKSFQKTFLKSPLNYRRISSHFSGGRRHPI
                     LKIVRPHLGVDFVAAEGTPVVAAADGVVIEKGYEGGGLGNFIRIRHKNPRFATVYGHL
                     SGFGPGIAIGKAVQQRDVVGYVGRTGLATGPHLHYCFYENGRPVNPLKIKNSSGDPIP
                     AGEMERFKGVREEQLALLAAAPRRGEEVPGVIAGTGPGHMAMPRL"
     gene            6408..7175
                     /locus_tag="PKM23_09260"
     CDS             6408..7175
                     /locus_tag="PKM23_09260"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNY91702.1"
                     /translation="MPAMLLTGASGFLGGHLLEKSSPHWNLTALSHTRPLPGTLSLDL
                     RDREAVLALVRARRPRVILHTAANANLDACQRHPEEAAAVNTTTVAILLEAAAEVKAR
                     LILVSTDMVFDGARGLYNEEDEVHPLSVYGATKVEAEHLVLAAPGSHLVARAALIYGR
                     PRHSGSSFSQWIENRLHNSQQVPLYTDQFRSPIWVENLADALLELAGMEYSGLLHLGG
                     ANRIDRYTFATQLCSAAGYDPALLQPVSMHTAVSDTG"
BASE COUNT         1390 a         2230 c         2129 g         1451 t
ORIGIN      
        1 cgccggaaca gacgatgaag cgatcacggg cgctgaggcc gagggaccat tcagtcaggc
       61 ggtgggccag ggcgcgagca gcctcgtaat actgagccag gcgcacggca tggctggctt
      121 tgtcgaattc acgctggaaa tccgcaccgg cggtaccgtt gcgctgcgat tgcgagagga
      181 cgctcttgag ctgcttctgg gcgacatcaa ggggcaggac ccgcgccgaa ccgtaaaaga
      241 ccaccgtatt gcggatgtgc cggcggcgca ggcgcatctg gggttcaaga tattcagaga
      301 ggatgcgaat ggtgcgcgca tccgagctgt tgagaaagtg ttcattggca taggctttga
      361 cgggcagttt ccgggctgcg cccggtttgg ggttcttttt ggggctgtcg gacatggcta
      421 cctcagttag gggtggttca gagattgcgt ttgagccaga tcagactctg acggatggcc
      481 tgatggcggt gcgccgggag cgcataggta tggctggcgg cggggagttc catgaaatcg
      541 gcggggggct gcacaatgtc acgaaaggag tcgaatccct ggatgaagac cggggcatcc
      601 gccatgccct gaacgcaaag cttgggcatg gtcagagcgc ccaatgccgc acggacatcg
      661 tagcggatgg catcctccca gaaagagcgc cggatcaccc agccctcatg gatatactcc
      721 tccgcttgtg cggagatggc gtcgagattg ctgatgtagg actgaaaggt cgcctgcatg
      781 tcgggcacgg ccgaccaggt gaccagggca tcgacagggg tctccgccag ggtgaggatg
      841 gcggtggccc cgcccatgct gtggcccagt acggcgattc tggcgaagcc gaggccgcgc
      901 gcccaggcga tggcatcaca cagattggcg atgtagaggc tgatcaggga atccccgaaa
      961 tccccggcgc tgtccccgga gccgtagaaa tcgaagcgca ggacgtcgag cccttcggcg
     1021 gcgaaggcgc ggccggtctc gacgaagagg cgccggcatt ccgcctttga actcataaag
     1081 ccgtgacaga ggatgaccag acgctccccc ctgctcttgt ggtgcatggc aacgatctga
     1141 tggccctggc tgagaaaggt aacggggagt tcgggaagag gctgcggcat agaaacgatc
     1201 ctgggtgtag ggtccgggca gtccgccatg gcgccggtcc gggcggaggc atggcccccc
     1261 ggcgggtgcg gaaaaagaaa aaaatagcgc tgactttgca gtcagcgcta gaaaagggtg
     1321 ttgtcccatc actacacatc actacccaca cccgacactg tgcctttcgg cacattaata
     1381 agttaagaat tttcaactca aagtcaagtt atttcttgcc ggctgtaaaa attggtcaaa
     1441 aattgaccat atgaggcccc ggcggagcct ggccccgggg ctgggacagt cggggcaaaa
     1501 aaaaagcccc cgcaagcggg ggcctgtgac gattagtaca tatctgcgcc gggaggcatc
     1561 ggcggggtcg ccggtttggg ctccggcttt tccgcgattg cgcactcggt catcaggaag
     1621 aggctcgcca ccgaggcggc gttctcaagg gcaatacgcg agaccttggt cgggtcgatg
     1681 acaccggcct tgtagagatc ttcgaaggtc tcggattcgg cattgaaacc gaaggcgccg
     1741 tcggtgtcgc gcaccttctg gaccacgatc gaaccttccc agccggcgtt ctcggcgatc
     1801 aggcggatgg gctcttcgag ggcacggcgc aggatggcta cgccgatctt ctcgtcaccg
     1861 ttgacctgga tcttgtccag ggcgggcagg gcgcgcaggt aggcgatgcc gccgccgggg
     1921 acgatgcctt cctcaaccgc cgcacgggtg gcgtgcaggg cgtcctcgac gcgggccttc
     1981 ttctccttca tctcgacctc ggtggcagcg ccgacctgga ggaccgcaac accgccggcc
     2041 agcttggcca gacgctcctg cagcttctcc ttgtcataat cggaagtcga ggactcgatc
     2101 tgcttcttga tctgggagat gcgagccttg atgtcctcgg tcttgccggc gccctcgacg
     2161 atggtggtgt tgtccttgtc gatggtaatg cgcttggcct tgccgagatc gctcatggtg
     2221 gtgttctcga gtttgaagcc ggcctcttcg gagatgacac ggccgccggt gaggacggcg
     2281 atgtcttcga gcatggcctt gcggcgatcg ccgaaaccgg gggccttgac tgcggcgacg
     2341 cgcagggtgc cgcgcagctt gttgaccacc agggtggcga gggcctcgcc ctcgatatcc
     2401 tcggcgatga tcagcatcgg acggccggtt tgggccacct tctcgaggat cggcagcaga
     2461 tccttcatgg cgctgatctt cttgtcatag atgagaacca tcacctcttc gaggaccgct
     2521 tccatggtgt cggaattggt gacgaaatag ggcgagatat aaccgcgatc gaactgcatg
     2581 ccctcgacaa cctcaagggt ggtatcggtg ctcttggcct cttcgacggt gatgacgccg
     2641 tccttgccca ccttttccat agcatccgcg atcagttcgc cgatcgcctt gtcgttgttg
     2701 gcggaaatgg cgccgacctg ggcgatctcg gtcttgccgg gaatcggctt gctctgcttg
     2761 cgcagctctt cgatgacgct cttgacgccc ttctcgatgc cgcgcttcag ggccatcgga
     2821 ttggagccgg cggtgacgtt tttcagacct tcacgcacga tcgcctgggc aagcacagtc
     2881 gccgtggtgg tgccgtcacc ggccacgtcg ctggtcttgg aggccacttc gcgcaccatc
     2941 tgggcgccca tgttctccag gggatcttcc agctcaacct ctttggccac cgtcacgccg
     3001 tccttggtca cggtcggggc gccgaacttt ttgtcgatca cgacattgcg gcccttgggg
     3061 ccgagagtcg aaccgaccgc tttggccagc ttgtccacgc cgaccttcat cgcattgcgc
     3121 gcatctacat tgaattcaat aatttttgcc atatgggtaa aacctcctga tggattgctt
     3181 tttaggttca catgccacgc ctagatgatt gcaaatatgt cgctctcgcg catgatcaga
     3241 tagtcttcct tgtcgatggt cacttcagtg ccggaatact tgccgtagag gatcctgtcg
     3301 cctaccttga ccgacatctc gatcttggcg ccattctcgg agatcttgcc cgggccgacg
     3361 gccacgatgg tgccctgcat gggcttctct ttggccgtgt caggaatgat gatgctcccc
     3421 tgcttcattt cctgctcttc gaccggcttc accacaacgc ggtcagccaa cggtttaatg
     3481 gtcatattgc atcctccaat cattattaat tgtttattat tatttaatca cgctagcact
     3541 ctctattact gagtgctaac aatctataat atacacattt taaaatcgca gtcaagcatt
     3601 ttttattccc ctgcgggccc gctttcccgc atggatgggc aaattgttgc agggaatttt
     3661 atttgccatt tttcgcttga cattggtccc cccagtggat atatttaaat tgatgtaaat
     3721 tcttttgtgc tttacatttt cgcgcaacac cgccaggcag caaaacccaa tccacggtcc
     3781 gattgcacga gctatcacgc atgcaggaag gggtgtacgg atgaaagaat ttgtcgagta
     3841 catcgtaaag catctggtcg acaatcccga tgaggtcaag gtctctgaag tattcggcga
     3901 aaccacggtc gtctacgaac tgcgtgtttc ccaacctgac atgggcaagg tcatcggcaa
     3961 aaacggccgc accgcccatg ccatccgggt acttttaagc gccgtctcac gcaaggcagg
     4021 caaacacgcg accctggaaa tccttgaata atttttttat atgcaggagc caagggggga
     4081 agcagccgga atgctgaagg acatagtccg ccccggctga ggcaggaacc tgagtaccat
     4141 cggaccatcg gaggctatct accggtggtc ccttttattt gtgataaccc tccccgcgca
     4201 ggatggtcag ggcccggaag agctgctcca gcagcacggt cagggcgagc tcgtgggcca
     4261 tggtcatcgc cgagagtgag aggcgctggt tcgccctcct caacacccca tcgcccaggc
     4321 cgtgcggccc gcccacaatg aagagcagag acttgcgccc ccccaccata tgccgctcca
     4381 gcgcggcggc gaaagccagc gaatccatcc cttcacccct gctctccagc accacgcacc
     4441 actcctgcgc cccgatcttc tcgagcagct gtgcctcttc ccgcgccagg atctccgcca
     4501 ccgctccggt cgcgcgcacc tgccgaatga aatgatgctc aaccggcaga tagtggcgga
     4561 tgcgtccgag atagccctcg cagagggcgt tgtaatgggg gtcggacggc ttgccgacag
     4621 taagaatgcg tatcttcatg gatggctccc ggggagatcc cggcgaaaag gctggattct
     4681 tttccggtgg aatggtatat tatccggtga gcaaaccagg ctcatgagca gagcagactt
     4741 gacgaggaga tcccgtaatg gctgatgacg acaagaacgc cggcgtgacc agcagccgga
     4801 tcaaattttg cgatttgcgc tgcgaacatg cctctttccc cagcgcggaa catcttgacg
     4861 gcgccaacag ctgccgcacc ttcgccgccc tctggtgccg tgagctgcag caggtcgtca
     4921 ccaaaaacgc cccctgcgcc ctcctctttg gtgcgcgccg ccccaagtcc aatctctgag
     4981 aaaacgcagc ccctccccgg ttttccccgc tacagcctgg gcatggccat atggcccggg
     5041 cccgtgccgg cgatgacacc cggaacctcc tcaccccggc gcggcgccgc cgcgagcaga
     5101 gccagctgct cttcccgcac ccccttgaag cgctccatct ctccggccgg gatcggatcc
     5161 cccgaggagt tcttgatctt gaggggattg accggccggc cgttctcgta aaagcagtag
     5221 tgcagatggg gcccggtggc cagaccggtg cggccgacat agccgacgac atcgcgctgc
     5281 tgcaccgcct tgccgatagc gatgccggga ccaaagccgc tgagatggcc gtagaccgtg
     5341 gcgaagcgcg gattcttgtg cctgatcctg atgaaattgc ccaatccgcc cccctcgtag
     5401 cccttttcga tcacgacccc gtctgcggcc gcaaccacgg gcgttccctc ggctgcgaca
     5461 aaatcaaccc cgagatgggg gcgcacgatc tttaaaatgg gatgccgccg gccgccgctg
     5521 aaatgagagc tgatccgtcg gtagttgagc ggggatttga ggaaggtctt ctggaacgac
     5581 ttgccctcga gatcgtaata gccgccgcgc tcgccctgct cgaaatagag ggcggtgcgc
     5641 accgcgctgc cggaacggta ctgcccggag agcaccctgc cataacggat gaattcaccg
     5701 aggctgtccg gccgggaggg atccagcgcg tactccgcct catagaccat gcggaattca
     5761 tcccccaccc gcggatcgac gaagaaatcg atgtcccact gaaaaaggtc gctgaaagcc
     5821 gcaatcagct catcgctctc ccccatcgcc ttgaccgcgt cataaagggt ggtcctgacc
     5881 tcacccgtga gggccagggt gcgtttgatg agcggcacct gcaccagctc gggctgcagc
     5941 acccctgtcg agtcgcgcag gacgcgcacg gtcttgacgg cgtccggata atactcaaag
     6001 ccatgcacaa ccccggccga atcggtcagg atggtgtaac gccggtctcc gcggatcttg
     6061 cgcacatcga agaccctggc gaaaccctgg ataagctcat cagcgaggac gcggtcatag
     6121 atgtcccgca gcaggacctt ttccagggtc tcgccggggc ggaaggtgcc ctcccggcgc
     6181 agatagatct tttcccgcgg cttgagctcg gcctgttcga ccggtcggtc ggatttcctc
     6241 gccgtccaca accacacggc cagaatggct gcggccgcaa gcaggagggg atagcgtact
     6301 ttatggagga tttctttttt catcgttgtc catttaaaaa aagcagattg cttcttaccg
     6361 gctgttttgc taccttgtag aaagacccta aacccggagc atccgtcatg cccgccatgc
     6421 ttctgaccgg tgcgagcggc tttctcggcg gccacctgct cgaaaaatcc tcaccgcact
     6481 ggaacctgac cgccctcagc cacacccggc ccctgcccgg gaccctgtcc ctcgacctcc
     6541 gcgaccgtga agccgtcctg gccctggtgc gcgcccggcg tccccgcgtc atcctgcaca
     6601 ccgcggccaa cgccaatctc gatgcctgcc agcgccatcc agaggaggcg gctgcggtca
     6661 ataccaccac ggtggcgatc ctactcgaag ccgcagcaga agtcaaggcg cgcctgattc
     6721 tggtctcgac cgacatggtc ttcgacggcg cccgcggact ctacaatgag gaggatgagg
     6781 tccatcccct ctccgtctac ggtgcgacca aggtcgaggc cgaacacctg gtcctggccg
     6841 ctccaggcag ccaccttgtc gcccgcgccg cattgattta cggccgcccc cgccacagcg
     6901 gcagctcatt ctcccagtgg atcgaaaacc gcctgcacaa cagccagcag gtgccgctct
     6961 atacagacca gttccgctca cccatctggg ttgaaaacct ggctgatgcc ctgctcgaac
     7021 tggccggtat ggagtactcc ggccttctcc acctcggcgg cgccaatcgc atcgaccgct
     7081 acaccttcgc gacccagctc tgcagcgccg ccggctatga ccccgcgctg cttcagccgg
     7141 tgtcgatgca cactgcggtc agcgacaccg gctgaagcag cgcggggtca tagccggcgg
//
LOCUS       DAOPWU010000167         7190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10398_length_7190_cov_5.075263, whole genome shotgun sequence.
ACCESSION   DAOPWU010000167 DAOPWU010000000
VERSION     DAOPWU010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10398_length_7190_cov_5.075263"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PKM23_09265"
     CDS             <1..455
                     /locus_tag="PKM23_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNY91703.1"
                     /translation="IETKLYLRTLIPESRGDVLGLLESSDKVLNQTTETLMQFSVECP
                     VIPEDLHPLFIDISDYAVSAEEGMVKAIRAYFRDLAAVRDHVNKVLFYRKEANKSAEK
                     FKRAVFSRDLRHSHKIHLRYFAYHVELIAEEAEDVCDRLSIATIKRYI"
     gene            467..1666
                     /locus_tag="PKM23_09270"
     CDS             467..1666
                     /locus_tag="PKM23_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006509240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNY91704.1"
                     /translation="MIWFFLTSGLFLGWSLGAKDAANVFGTAVGSRMVRFKTAAAVAS
                     LFVILGAVVEGAGTTATLGHLGAVNAIAGSFTVALAAGVTIAWMTRARLPVATSQAIV
                     GGIVGWNLFTGSPTDLGVLSRIVATWLLSPLLAAIFAILLYLLFKRCLGRARIHMLRL
                     DAYTRTGLIIVGALGSYTLGANNIANVMGVFVPASPFRDIQLFGIFTLHGTQQLFLLG
                     ALAIAVGIFTYSRRMMDSVGNDIFKLTPILAFIVVLAHTIVLFLFSSTGLQSALVALG
                     LPTIPLVPVSSSQAIVGAIFGIGIVKGAQGINFKLLGKIAAGWVMTPIFAGLLTFVSL
                     FFVQNVFEQEVVHHPAAAATSIIAPEPRPNDTAPVTPLASPMQASAGNDSLITINSSN
                     PLPQPIN"
     gene            1676..2971
                     /locus_tag="PKM23_09275"
     CDS             1676..2971
                     /locus_tag="PKM23_09275"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNY91705.1"
                     /translation="MDTHKLYLRTALVLLFAAALLFGASETLWASDAKVPGGDEKIAL
                     KSQARNGKMVFETEDGAYRWWFDSRVQFDGAYFFENKNDMSNSFVLRRVTFALKAQLA
                     RDWEAEVDVDFGERVYPEANLDARDMWVKYSVPNTGLAFTVGNFKEPFGMERLNSSRL
                     LTFLERSAPTNAFALGRRIGVAGRYYNKYGQLTLAAMGHELGSRIDKGQRDDGMSGNL
                     RLTAAPINQHGKNLHVGFAFSSKNPETLPDLAENTIEIRARTENYIFDPKQLHTGDIK
                     DVNYYTRYGYELMGIKGPFYFQGEAFTTDIFRWYDKPEVNLRGAYATVAWLVTGETRY
                     YYIDEGEVGPIEKPNKDWGALEVAARFSYTDLNDLGAGVHGGSSKQLMLGVNYYPNTN
                     IKLQFNYSIVDLDQYATRKGNLFGDDDHSFVQMRVQASL"
     gene            2990..3913
                     /locus_tag="PKM23_09280"
     CDS             2990..3913
                     /locus_tag="PKM23_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNY91706.1"
                     /translation="MKIRPLLIALAILVALLAISCSREAPVQPPPPPPPVYISLNEVY
                     SRGTDADPDWIEIYNSSAEQQDVSGYKLYDSGGKEGTKPKLSLPAGTVISGLGLLVVV
                     VDDTTDAGFGLSSGGEEVWLEDATGKVIDSVVFPALEATQSYGKTLEGSQEWKVWEQV
                     TRGLGNTGTDVIILPVKMNEAFSRGVEGNLDWIEIYNPSNVAISLAGYQIYDNGGQSG
                     VKPKLVFPAGASAPARGFYVITVDDTSAAGFGLSSGGDEIWLEDPTNVLIDNVKIPAM
                     PVETTSYCRIPDGSETWQISNTITKGAANLP"
     gene            3982..4743
                     /locus_tag="PKM23_09285"
     CDS             3982..4743
                     /locus_tag="PKM23_09285"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HNY91707.1"
                     /translation="MKIKLLSFTAALFILAACSREDPNKSTNGPDASQPAALKPLQAI
                     SLLPAVPEPSGLAYNRATGGLMVVSDALPDIFEIGLDGALRRTIPVAGEDLEGVAVSL
                     TGDSLYVVEERLQQVVRCSSAGERLMSWPARVATMENSGLEGIAIDAAGHIWVANEKD
                     PRLLLEFNGTQEVSRREITWVDDLSGLCCDQDGQSLWIISDESRKLIHISRAGALLGE
                     WSLPFDKGEGIAFAGDKLYIVNDQDSRLYIFNKPN"
     gene            4811..6457
                     /locus_tag="PKM23_09290"
     CDS             4811..6457
                     /locus_tag="PKM23_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNY91708.1"
                     /translation="MKRATRVVTLLLGIAMVAFLVSGCLITEVIQSLVVAAGGEFTTK
                     VTITDKTADANPHEGALAILTPDDWEFVSGTYDSQAGKGAFIADPNTPPVYGDLDGKL
                     PAPAGMKWIRLLTDAAYTNEANVVHEANITLKVGSKTGDFMIGYMATKNSVDMLGSIN
                     PLNEDNDNAWADTSMNHKVTVSGAAAAAVIFNEAFSRGTADAPDYVELYNPSALPFDV
                     SGYKVYDNGGQAGSKPKKEIPAGTVIAPWGFLAISVDNTGADSDFGLSSGGDKVWLET
                     PAGAVIDSVEFGAMQPTESWQRIPDGGPWKLSTPLTRGTSNLPLKLNEAFSRGTDTDP
                     DWVELYNSCADTLDISGYKVYDNGGQAGTKPKKEVPAGTKLAPKGFLVIVVDKTGADS
                     DFGLGSGGDKVWLEDAVGAVIDSVEFGALETTQSWARKPDAGLWQLSTIITKGFTNGD
                     PTGVATAPRVVEGYLLTQNYPNPFNPSTRIDFTLPRQAEVQVAVYNLRGEMVAELVNG
                     RLAAGSHQLTFNAEGLASGIYIYTIRSGEFAAAKRMVLMK"
     gene            6644..>7190
                     /locus_tag="PKM23_09295"
     CDS             6644..>7190
                     /locus_tag="PKM23_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNY91709.1"
                     /translation="MINQNTVDLELPPGRVALDLLRGEFGLTGVKEGCREGDCGACTI
                     LLGRLHDGHIAYHSVVSCLLPIGDCAGKHLVTIEGLNGPGLNPLQQAFVEEGAVQCGF
                     CTPGLVLALTAWLLNAPDWNEEEALLSIAGNICRCTGYLAIRRAVRRIIAALPAAAEG
                     TRVETLIAAGYLPAWFREAPAQ"
BASE COUNT         1393 a         2439 c         1979 g         1379 t
ORIGIN      
        1 agatcgagac caagctctat ctgcgcaccc tgattcccga atcgcgcggg gatgtgctcg
       61 gcctgctcga gagctccgac aaggtgctca accagaccac agagaccctg atgcagtttt
      121 cggtcgaatg cccggtcatc cccgaggatc tccatcccct ctttattgat atctccgact
      181 atgctgtcag cgccgaggag gggatggtca aggccatccg cgcctatttc cgtgacctcg
      241 ccgccgttcg cgaccatgtc aacaaggtgc tcttttaccg caaggaggcc aacaagagcg
      301 cggagaaatt caagcgcgcc gtctttagcc gggatctgcg ccacagccat aaaatccacc
      361 tccgctactt cgcctatcac gtcgagctga tcgccgaaga ggccgaggac gtctgcgacc
      421 ggctctcgat cgccaccatc aaacgctata tctgagcacg cacggcatga tctggttttt
      481 cctgacaagt ggtctttttc tgggctggtc cctcggcgcc aaggacgccg ccaacgtctt
      541 tggcacagca gtgggctcgc gtatggtgcg cttcaagacc gccgcggccg tcgccagcct
      601 cttcgtcatc ctcggggcgg ttgtggaggg cgccggcacc accgctaccc tcggccacct
      661 cggggcggtc aacgccatcg cgggctcctt cactgtcgcc ctcgccgctg gtgtgaccat
      721 cgcctggatg acgcgcgcca ggctgccggt ggcgacctcg caggccatcg tcgggggcat
      781 cgtcggctgg aatcttttca ccggctcccc gaccgatctg ggggtgctct ccaggatcgt
      841 cgcgacctgg ctgctctccc ccctgctcgc agcgatcttt gccatactgc tctatctgct
      901 cttcaagcga tgtctcggcc gggcacgcat ccacatgctc cgcctcgacg cttatacccg
      961 caccggcctg atcatcgtcg gcgctctcgg ctcgtacacc ctgggcgcca ataatatcgc
     1021 caacgtcatg ggcgtctttg tgcccgcctc cccgttccgc gatatccagc ttttcggcat
     1081 cttcaccctg catggcaccc agcagctctt cctgctcggg gcactggcca tcgcggtcgg
     1141 catctttacc tattcccgcc ggatgatgga ttccgtgggc aacgacatct tcaagctcac
     1201 ccccatcctg gccttcatcg tcgtcctcgc ccataccatt gtgctctttc ttttttcctc
     1261 caccggcctg cagagcgccc tcgtcgccct cggcctgccg acgattcccc tggtgccggt
     1321 ctcgagttcg caggcgatcg tcggggcgat cttcggaatc ggcatcgtca agggggctca
     1381 gggcatcaac tttaaactgc tcggcaagat cgccgccggc tgggtgatga cccccatctt
     1441 cgccggcctt ttgacctttg tttcgctctt ttttgtccag aatgttttcg aacaggaggt
     1501 ggttcaccat cctgctgcag ccgcaacaag catcatcgca ccggaacccc ggcccaacga
     1561 cacagccccg gtgacaccgc ttgcctctcc gatgcaggcc agtgccggaa atgacagcct
     1621 gattactatc aatagctcca atccattacc ccaacccatt aactgaggag aaaccatgga
     1681 cactcacaaa ctctacctgc gtaccgcgtt ggtgctgctc ttcgccgccg ccctgctctt
     1741 cggcgcgagc gaaacactct gggcatccga tgccaaggtt cccggcggcg atgaaaagat
     1801 tgccctcaaa tcccaggccc gcaacggcaa gatggtcttc gagaccgaag acggcgccta
     1861 ccgttggtgg ttcgattccc gcgttcagtt tgacggcgcc tacttttttg agaacaagaa
     1921 cgacatgagc aacagcttcg tcttgcgccg cgtcaccttc gccctcaagg cgcagctggc
     1981 cagagactgg gaggctgagg tggatgtcga tttcggcgag cgtgtatatc cagaagccaa
     2041 cctcgatgcc cgcgacatgt gggtcaagta cagcgtgccc aacaccggcc tggctttcac
     2101 agtcggcaat ttcaaggagc ccttcggcat ggagcgcctc aacagctcgc gcctgctcac
     2161 cttcctcgaa cgttcggccc cgaccaacgc cttcgccctc ggccgccgca tcggcgtcgc
     2221 cggccgctat tacaacaagt acggccagct caccctcgcc gccatgggcc acgaactcgg
     2281 ttcccgcatc gacaagggcc agcgcgatga cggcatgtcc ggcaacctcc gcctcaccgc
     2341 cgcccccatc aaccagcacg gcaagaacct ccacgtcggc ttcgccttct cctccaagaa
     2401 tcccgagacc ctgccggacc tggctgaaaa taccatcgaa atcagggcgc gaaccgaaaa
     2461 ctacatcttc gatcccaaac agctccatac cggcgacatc aaggatgtca actattacac
     2521 ccgctacggc tatgaactga tgggtatcaa ggggcccttc tacttccagg gcgaggcctt
     2581 caccaccgac atcttccgct ggtacgacaa gccggaggtt aacctccgcg gcgcttacgc
     2641 cacggtcgcc tggctggtga cgggcgagac ccgctactat tacatcgacg agggcgaagt
     2701 cggtccgatc gaaaagccga acaaggactg gggcgccctc gaagtggcgg cgcgcttcag
     2761 ctacaccgac ctcaatgatc tcggcgccgg ggttcacggc ggctcctcca aacagctcat
     2821 gctcggcgtc aactattacc ccaacaccaa catcaagctg cagttcaact actcgatcgt
     2881 cgatctcgac cagtatgcca ctcgcaaggg caacctcttc ggagacgacg accactcctt
     2941 tgtccagatg agagtccagg cttccctgta accgatagga gaatgcacca tgaaaatacg
     3001 tccattactc atcgcgctgg ccattttagt cgccctgctg gcgatctcct gctcgcgcga
     3061 agcccctgtg cagccgcccc ctccgccacc gccggtctat atctcgctca atgaggtcta
     3121 ttcgcgcggc accgacgctg atcccgactg gatcgaaatc tacaactcct ccgccgaaca
     3181 gcaggatgtg agcggctaca agctctacga cagcggcggc aaggagggca ccaagcccaa
     3241 actgagcctg cccgccggca ctgtgatttc cggcctcggc ttgctcgtgg ttgtggtcga
     3301 cgacaccacc gacgccggtt tcggcctctc cagcggcgga gaagaggttt ggctggagga
     3361 tgccaccggc aaggtgattg acagcgtggt ctttcccgct cttgaagcca cccagtccta
     3421 cggcaagacc ctcgagggca gccaggagtg gaaggtgtgg gagcaggtca cccgcggcct
     3481 cggcaatacc ggcaccgatg tcatcatcct gccggtcaag atgaacgaag ccttttcacg
     3541 cggtgtggag ggcaacctcg actggatcga gatctacaat ccctccaatg tcgccatctc
     3601 tctggccggc taccagatct atgataacgg cggccagagc ggggtcaaac ccaaactggt
     3661 cttcccggcc ggagcctccg cgcccgccag gggtttttac gtcatcaccg tcgacgatac
     3721 ctccgccgcc ggcttcggcc tctccagcgg cggcgacgag atctggctcg aagaccccac
     3781 caacgtgctg atcgataacg tcaagattcc ggccatgccg gtggaaacca cctcttactg
     3841 ccgtattccg gatggttcgg aaacctggca gatctccaat accatcacca agggcgctgc
     3901 caacctgccc tgaccccggt cttcatcccc aaccggtgtc cgcgcacggg cgcggacacc
     3961 ggactaattc aggtcgactc tatgaaaata aagctcctct cttttaccgc cgctctgttc
     4021 attctggccg cctgttcgcg tgaggacccc aacaaaagca cgaacggacc tgacgcctcc
     4081 cagccggctg ccctcaagcc gctgcaagcc atcagcctgc tgcccgcggt tcccgagccc
     4141 tccggactcg cctataaccg tgccaccggc ggcttgatgg tggtctctga tgcccttccc
     4201 gatatttttg agatcggcct cgacggcgcc ctgcgccgca ccatcccggt cgccggagag
     4261 gatctggagg gggtggccgt ttcgctcacg ggggattccc tttatgtggt cgaggaacgc
     4321 cttcagcagg tcgtgcgctg ttcttcggcc ggggaacggc tgatgagctg gccggcccgg
     4381 gtcgccacca tggagaacag cggcctggaa gggatcgcca tcgatgcggc cggccacatc
     4441 tgggtcgcca acgagaagga tccccgcctg ctgctcgagt tcaacggcac acaggaggtg
     4501 agccggagag agatcacctg ggtggatgac ctctccggat tgtgctgcga tcaggacggc
     4561 cagagcctgt ggatcatcag cgacgagtcg cgcaagctta ttcacatctc gcgcgccggc
     4621 gccctgctcg gcgaatggtc gctgcccttt gacaagggtg agggcatcgc cttcgccggc
     4681 gacaagctct acatcgtcaa cgatcaggac agcagactct acatttttaa caagccgaat
     4741 tgatacggaa tatgcttgtg cattagcgca tggttttgta aattagaaaa aacagtataa
     4801 ggaggcaggg atgaaaagag ctacaagagt tgtgacgctt cttctgggga ttgcaatggt
     4861 ggcctttctg gtcagcggct gtctgatcac cgaagtcatc cagtcactgg tcgtggccgc
     4921 cggcggtgaa tttaccacca aggtgactat caccgacaag acggcggacg ccaatcccca
     4981 tgaaggcgcc ctggccattc tcaccccgga tgactgggaa ttcgtatccg gcacctatga
     5041 cagccaggcg ggcaaagggg ctttcatcgc cgatcccaat acccccccgg tctatggtga
     5101 tctcgacggc aaactgccgg ctccggcggg catgaaatgg atccgtctgc tgaccgatgc
     5161 ggcctatacc aatgaggcca atgtggtcca tgaagccaac atcaccctca aggtgggcag
     5221 caaaaccggc gatttcatga tcggctacat ggccaccaaa aacagcgtcg acatgctcgg
     5281 cagcatcaat ccgctcaatg aagacaacga taatgcctgg gccgacacct ccatgaacca
     5341 caaggtcacc gtttccggcg cggccgctgc ggcggtcatc ttcaatgagg ccttcagccg
     5401 cggcaccgct gatgccccgg attacgtcga gctttacaat ccctcagctc tgcccttcga
     5461 cgtcagcggc tacaaggtct atgacaacgg cggccaggcg ggcagcaagc ccaaaaaaga
     5521 gatccccgcc ggcaccgtga tcgccccatg gggctttctg gccatcagcg tcgataacac
     5581 cggtgcggac agcgatttcg gcctcagcag cggcggcgac aaggtctggc tggagactcc
     5641 ggccggtgcg gtcatcgatt cggtcgagtt cggcgccatg cagcccaccg aatcctggca
     5701 gcgcattccc gacggcggcc cctggaagct ttccactcca ctcacccgcg gcacctccaa
     5761 tctcccgctc aagctgaatg aggccttttc ccgcggcacc gacaccgatc cggactgggt
     5821 cgagctctat aacagctgcg ccgacaccct cgacatcagc ggctacaagg tttatgacaa
     5881 cggcggacag gccggcacca agcccaagaa agaggttcct gcgggcacca aattggctcc
     5941 caaaggcttt ctcgtcatcg tcgtcgacaa aaccggcgcg gacagcgact ttggcctcgg
     6001 tagcggcggc gacaaggtct ggctggaaga cgccgtcggc gcggtgatcg actcggtcga
     6061 attcggtgcc ctcgaaacca cccagtcctg ggccagaaag ccggatgccg gcctctggca
     6121 gctctccact attatcacca aaggcttcac caatggcgac cccaccggtg tggccactgc
     6181 gcccagagtg gttgagggct atctgctgac ccagaactat cccaatccct tcaatccatc
     6241 caccagaatc gatttcaccc tgccccgtca ggctgaggtc caggtggcgg tctacaatct
     6301 gcgcggcgag atggtcgccg aactcgtcaa cggccgcctg gccgccggtt cgcaccagct
     6361 caccttcaat gccgagggat tggcgagcgg catctatatc tataccatcc gcagcggaga
     6421 gtttgcggcc gccaaacgta tggttctgat gaagtagtgg agcgcacctg cactctgctg
     6481 taatactgca tcactttctg agagagccat cacggcagcg gtcgtgatgg ctctccgttc
     6541 atcgggctgc cccgctgcgg cccggactct ccggctgaag gaccagacat ctgcggccgg
     6601 aactctaccc agggatgcgg aggatgggcc ccaaatgcgg ttgatgatca accaaaacac
     6661 cgtcgatctg gaactccccc cggggcgggt cgctctcgac ctcctgcgcg gcgagttcgg
     6721 cctcaccggc gtcaaggagg gctgccgcga aggcgattgc ggcgcctgca ccattctcct
     6781 cggtcggctc catgacggcc acatcgccta ccactccgtc gtctcctgcc tgctccccat
     6841 cggtgactgc gccggcaaac atctggtcac catcgaaggc ctcaacggcc ccggactcaa
     6901 tcccctccag caggccttcg tcgaagaggg ggcggtgcag tgcggcttct gcacccccgg
     6961 acttgtcctc gccctcaccg cctggctgct gaacgcgccg gactggaacg aggaagaggc
     7021 ccttctcagc atcgccggca acatctgccg ctgcaccggt tatctcgcca tccgccgcgc
     7081 agttcgccgc atcatcgccg ccctgcctgc cgccgcggag ggcacgcgcg tggaaaccct
     7141 catcgcggcc ggatatctgc cggcctggtt cagggaggcg ccggcgcagc
//
LOCUS       DAOPWU010000168         7186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10405_length_7186_cov_4.883887, whole genome shotgun sequence.
ACCESSION   DAOPWU010000168 DAOPWU010000000
VERSION     DAOPWU010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10405_length_7186_cov_4.883887"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKM23_09300"
     CDS             <1..410
                     /locus_tag="PKM23_09300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNY91710.1"
                     /translation="HAPTFSTLIAGLTLFIAIVAQMARLPFDIVEADQEIMEGPFVEQ
                     SGPKLALFKWMIYAKQFIFAAVFISVFIPWPVVQPAILNLLLTLLKMAVLLLIVGVVH
                     VVNPRLRIDQAVRFFLALVVIEIAGLALALVGS"
     gene            443..934
                     /locus_tag="PKM23_09305"
     CDS             443..934
                     /locus_tag="PKM23_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY91711.1"
                     /translation="MLKSKFKEARICFSNLRVTLPYPFGPGSEVAEGFRGKIDVDASR
                     CIGCGGCANVCPSRLIRCHDEGDQTHMEFILDRCTYCGRCAEVCPEGAITMTLLFESA
                     TDDKTDLYIEQQLYMLTCKRCGRCFETENAIDRIPTRRMRKGRNYVGSSMPHGAPGER
                     EAS"
     gene            931..1593
                     /locus_tag="PKM23_09310"
     CDS             931..1593
                     /locus_tag="PKM23_09310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HNY91712.1"
                     /translation="MITGQTLLTLSMAILITAMATVEIRTLRIAAWGYLVNSLLLCLL
                     IAACGRLYGNHGLYLWATTCLIFKVLLIPWLLLRFARGLPRIEHKPIPGFVLSMILMT
                     VIIFVLFQVFRGSIAGVAPSVREEPLRSLFAGAATLFSLGIWTLLSRRDLIKIAIGLA
                     LMENGVHMILLALAPQLRESTMIGILINAALAVFMLLYLGADIYRVFGTTDSALLSEL
                     KR"
     gene            1604..3094
                     /locus_tag="PKM23_09315"
     CDS             1604..3094
                     /locus_tag="PKM23_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNY91713.1"
                     /translation="MAQIDSILPLLLILWPLLGALLLLPAGRLRWGREAAAILVMIVE
                     LALSIRMAAASLTGQVLISGAGLRADGLSALMGLLINGVVLLVLIQAARYMRKEEEHG
                     LANRKSLLLFYILILIFTAAMNWTVTTNHLILLYVAMEASTLASALLVAFYRKRASLE
                     AGYKYVLLVIVGMTFSLLGMVLLYAAAQPLLGAQALLLTHVGQVARLVPKPIALAGVG
                     LMTVGFATKAGVVPFHAWLPDAHSEAPTPVSALLSGLIIKLGAYGLARTVAIFAPLLS
                     GIAPFIALLASVSMFTGIIMALVQDDLKRMLAFSSVSQISYVIIGLGLGTHLGIYSGL
                     FHMVNHTLIKALLFLSVGAVMYATGGERRISRLGGLAARMPVTSFCFILGALALGGLP
                     PLNAFLSKFSLFLALGEAHLWWAVAAAILTGLLTLACLVRAGYKVFWAPPADPEAYEA
                     ARVREVPFMLYFSMAILAGLCLLTGLCPNILYPLLEGATRAVMTVW"
     gene            3129..3629
                     /locus_tag="PKM23_09320"
     CDS             3129..3629
                     /locus_tag="PKM23_09320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNY91714.1"
                     /translation="MFKNLCAKAFPKALWVYHTNCAACNGCDIEIINVLTPYYDAERF
                     GIKLVGSPRHADVLLVTGGVSRQIAPALERLYAATPDPKLVFAIGSCACGGDVFYDSY
                     NMYGGVDKVIPVNFYVSGCPPRPEAILYGVAVALGLAPKKVAKKMISDLPDTMDLNPP
                     MLELRK"
     gene            3757..5811
                     /locus_tag="PKM23_09325"
     CDS             3757..5811
                     /locus_tag="PKM23_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNY91715.1"
                     /translation="MDPIVTTIENRCKRCYLCVRRCPAKAIRVENGQAKVMAERCITC
                     GYCVRVCPQHAKRIRDGIVHTHEMLGGEEETIALLAPSFPAAFTGVRPLQVVEGLHRL
                     GFARVYEVALGADLVGAEYARLKEADIMPIIITSPCPAIVNFIEKYYPELLLVLAPVV
                     SPMVALARLIKAQWHPQTRLVFIGPCIAKKREMDDPRTGGLIDEVLTFEELAALFRSE
                     KIALNRLPERELDGPEAGIGRSFAVSGGLLDTARIPGGTLQNKVIVTEGRDRILDALH
                     KVSEGNIEARFLDLLFCEGCINGPQMDNELSGFVRKDKVIHFLHHSAESTPFEKRSGW
                     MDGIKPVDLSREFTREEISLPLPSDDELAEILAITGKYSPEDELNCGACGYPTCREKA
                     IAVYQGIAEAGMCLPYLIDQLQKTEGELTASNVELKKSIETLRRTQAQLLQSEKMASV
                     GELAAGVAHELNNPLGGILIYTSLLLEKAASGAQEREDLELIRAETERCKRIVRGLLD
                     FSRQTRIEAAIADLNQILGGTLALVTQQALFHNVKVEQDLLPGLPKVFVDIGQIQQVL
                     LNIILNAVEAMHEKGTLIITTTWDPAKQQVLLTIRDTGTGMSPELQARIFEPFFTTKP
                     RGIGTGLGLSIAYGIIQRHHGEILVRSAPGEGSEFTIVLPEAEKMVMTDIELAGIEKD
                     QA"
     gene            5823..6266
                     /locus_tag="PKM23_09330"
     CDS             5823..6266
                     /locus_tag="PKM23_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY91716.1"
                     /translation="MAEQGKILLVDDDPDFNIINRTILESDGYSVEEARDPESAWEKI
                     RSWKPDLICLDVMMPTGTEGFHFAYKVRRDEATRHIPILMITAIHQHSDFRFSTDDGE
                     YLPVDDFVEKPIKRELLLAKVKALLAATRPALPKPADDKGIGLKK"
     gene            6389..6802
                     /locus_tag="PKM23_09335"
     CDS             6389..6802
                     /locus_tag="PKM23_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY91717.1"
                     /translation="MEKKKRILLVDDDPDFVEINRMILEASDYEVDAADSVALALQQL
                     EQNSYDLLIIDLMMEERDSGFTLTYAVRSDERLRDLPILMLTSAQEKTGFSFLLSEDQ
                     EWMKVDDFAAKPLKPAELVARIEKLTGKGAQDAKP"
     gene            6789..>7186
                     /locus_tag="PKM23_09340"
     CDS             6789..>7186
                     /locus_tag="PKM23_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY91718.1"
                     /translation="MQSREARILVVDDEEIIRIGCQRILQNPGFTIDLAENGRVGWEK
                     IQQKSYDMVLVDLMMPEMGGLEVLEKVQAHDEQIITIIITGFATIETAVDAMRKGAYD
                     YLPKPFSPEELRTKIRRGLEKRWLLLEAEEL"
BASE COUNT         1325 a         2327 c         2084 g         1450 t
ORIGIN      
        1 cccatgcccc cactttttca accctgatcg cgggactcac cctcttcatc gcgatcgtgg
       61 cacagatggc gcgtctaccc ttcgatatcg tcgaggccga ccaggagatt atggagggcc
      121 cctttgtcga gcagagcggc cccaagctgg ccctcttcaa atggatgatc tacgccaagc
      181 agttcatctt cgctgcggtt ttcatatcgg ttttcatccc ctggccggtg gtccagccgg
      241 ccatcctgaa tctgcttttg accctgctca agatggcggt gctgctgctc atcgtcggcg
      301 tcgtccacgt cgtcaatccg cggctgcgca tcgatcaggc cgtgcgtttt ttcctcgccc
      361 tggtcgtcat tgaaatcgcc ggcctggccc tggccctggt cggctcatga gaggtacaca
      421 ctgagactgg caggctgata ctatgctcaa atccaagttt aaagaggccc ggatctgctt
      481 cagcaatttg cgcgtcaccc tgccctatcc cttcgggccc ggctccgaag tggccgaggg
      541 attccgcggc aagatcgatg tcgacgcctc caggtgcatc ggctgcggcg gctgcgccaa
      601 cgtctgcccc tcgcggctga tccgctgcca cgatgagggc gaccagacgc acatggagtt
      661 catccttgac cgctgcacct actgtggacg ctgcgccgag gtctgtccgg agggggcgat
      721 caccatgacc ctgctctttg aatcggcaac cgacgacaag accgaccttt atatcgagca
      781 gcagctctac atgctcacct gcaagcgctg cggacgctgc ttcgagaccg aaaacgccat
      841 cgaccgtatc cccacgcggc gcatgcgcaa ggggcgcaac tatgtcggct cctccatgcc
      901 ccacggcgcg ccgggggaga gggaggcctc atgatcaccg gccagaccct gctcaccctg
      961 tcgatggcga tcctcatcac cgccatggcc actgtcgaaa tccgtactct gcgtattgcc
     1021 gcctggggct atctcgtcaa ttccctgctc ctctgcctgc tcatcgccgc ctgcggccgc
     1081 ctctacggta atcacggcct ctatctctgg gcaacgacct gtttgatctt caaggtgctg
     1141 ctcatcccct ggctgctgct ccgtttcgcc cgcgggctgc cccgcatcga gcacaagccg
     1201 attccgggct ttgttctctc catgatcctg atgacggtga tcatttttgt gctttttcag
     1261 gtttttcgcg gctcaatcgc cggggtggcc ccctctgtac gcgaggagcc cctgcgcagc
     1321 ctgttcgccg gggctgcgac ccttttcagc ctcgggatct ggaccctgct ttcgcgccgt
     1381 gatctcatca agatcgccat cggcctcgcc ctgatggaga acggcgtgca catgatcctg
     1441 ctcgccctcg ccccgcagct cagggagagc accatgatcg ggattctcat caatgctgcc
     1501 ctggccgttt ttatgctgct ctatctgggg gccgacattt acagggtttt cggcaccacc
     1561 gattcggcgc tgctctcaga gctcaagcga taacgaggtg accatggcac agatcgattc
     1621 catactcccc ctgctcctga ttctctggcc tcttttgggc gccctgctcc ttctgcccgc
     1681 cggcaggctg cgctggggcc gggaggcagc agccatcctg gtcatgattg tggaactggc
     1741 cctctccatc cgcatggcgg ccgcctccct gaccggccag gtcctgatct ccggcgccgg
     1801 cctgcgggcg gacggcctca gcgccctgat gggactcctg atcaacggcg tggtgctcct
     1861 cgtcctcatt caggcagcgc gctatatgcg gaaagaggag gagcacggcc tggccaatcg
     1921 caagagcctg ctcctcttct acatcctcat cctgatcttt accgccgcga tgaactggac
     1981 agtcaccacc aatcacctca tcctcctcta tgtcgccatg gaggcctcta ctctcgcctc
     2041 ggccctgctc gtcgcctttt accgcaaaag ggcctcgctg gaggcgggct acaagtatgt
     2101 actgctggtc attgtcggca tgaccttcag cctgctcggc atggtgctcc tctacgccgc
     2161 cgcccagccc ctgctcggcg cgcaggcgct tctgctcacg catgtcgggc aggtggcgcg
     2221 gctggttccg aagcctatcg ccctcgccgg agtcggactg atgacggtgg gctttgccac
     2281 caaggctggc gtggtcccct ttcatgcctg gctgccggac gcccactccg aggcccccac
     2341 cccggtcagc gcccttctct ccggcctgat catcaagctg ggggcctacg gcctcgcccg
     2401 caccgtcgcc atcttcgccc cgcttctgag cggtatcgcc cccttcatcg ccctgctcgc
     2461 ctcggtgagc atgttcaccg gcataatcat ggctctggtg caggatgacc tcaagcgcat
     2521 gctcgccttt tcaagcgtca gccagatcag ctatgtcatc atcggattgg gactgggcac
     2581 ccatctcggg atctacagcg gactctttca catggtcaac cacaccctga tcaaggccct
     2641 gctcttcctg agcgtcggtg cggtcatgta cgccaccggc ggggagcggc ggatctcgcg
     2701 gctggggggt ctcgctgccc gaatgccggt cacctcattc tgcttcatcc tcggcgccct
     2761 ggccctcggg gggctgccgc ccctgaatgc ctttttgagc aagttttcgc tctttctcgc
     2821 cctcggcgaa gcccatctct ggtgggccgt tgcggccgca attctcaccg ggctgctcac
     2881 cctggcctgc ctggtgcgcg ccggctacaa ggtcttctgg gcccccccgg ccgatcccga
     2941 ggcgtatgag gccgcccggg tgcgcgaggt gccctttatg ctctatttca gcatggcgat
     3001 cctcgccggt ctctgcctcc tgaccggcct ctgcccgaac atcctctatc cgctgctgga
     3061 gggggccacc cgcgcggtga tgaccgtctg gtaacaggag agacgcattc catttgaaag
     3121 gttcaggcat gttcaagaat ctgtgcgcga aggccttccc caaggcactg tgggtctacc
     3181 acaccaactg tgcggcctgc aacggctgcg acatcgagat catcaatgtg ctcaccccct
     3241 attacgatgc tgaacgcttc ggcatcaagc tggtgggctc gccgcgccat gccgatgttc
     3301 tgctggtgac cggcggggtc tcgcgccaga tcgccccggc cctggaacgc ctctacgcgg
     3361 ccacacccga ccccaagctg gtcttcgcga tcggctcctg cgcctgcggc ggggatgtct
     3421 tctatgacag ctacaatatg tacggcgggg tcgacaaggt cattccagtc aatttctacg
     3481 tctcgggctg tccgccccgg ccggaggcga tcctttacgg agtcgctgtc gccctcggac
     3541 ttgcccccaa aaaagtggcc aaaaagatga tcagcgacct cccggacaca atggatctca
     3601 atccccccat gctggagctg cgcaaatagg gttgcgttcc atgttctttt tcgctatttt
     3661 accagtggat cactgcggtc agcaggagat cgggccccct cccggacagg cagcaggggg
     3721 agggcggatt tcagccgtcg catgcttggg caaccgatgg atcctattgt aaccaccata
     3781 gagaaccgct gcaagcgctg ctatctctgc gtccgccgct gccctgcgaa ggccatccgc
     3841 gtcgaaaacg gacaggccaa ggtgatggcg gagcgctgca tcacctgcgg ttactgtgtg
     3901 cgcgtatgcc cccagcacgc gaagcgtatt cgcgacggga ttgtgcatac ccacgagatg
     3961 ctcggcgggg aggaggagac catcgccctg ctggcgccat cctttcccgc ggcctttact
     4021 ggcgtgcgcc cgctgcaggt ggtggagggg ctgcacaggc tcggcttcgc cagggtctat
     4081 gaggtggccc tgggggcgga tctggtcggt gctgagtatg cccggctaaa agaggccgac
     4141 atcatgccga tcatcatcac ttcgccctgc cctgcgatcg ttaactttat cgaaaaatac
     4201 taccccgaac tcctgctggt tctggcgcct gtagtctcgc caatggtggc cctggcgcgg
     4261 ctgatcaaag cccagtggca cccgcaaacc cgcctggtct tcattggccc ctgcattgct
     4321 aaaaagaggg agatggatga ccccagaacc ggcggcctta tcgatgaggt gctgaccttc
     4381 gaagagctgg cggccctctt ccggagtgaa aaaatcgctc tcaaccggct gccggagcgg
     4441 gagctggatg ggcccgaggc gggcatcggc aggtcctttg ccgtctctgg aggtctgctc
     4501 gataccgccc gcatcccagg cggaaccctg cagaacaagg tgatcgtcac cgaggggcgg
     4561 gaccgcatcc tcgacgccct gcacaaggtc agtgagggca acatcgaggc ccgctttctc
     4621 gatcttctct tctgtgaggg ctgcatcaac ggcccgcaga tggacaacga gctttcgggc
     4681 tttgtccgca aggacaaggt gatccatttt ctccatcaca gcgcggagag cacccccttc
     4741 gaaaagcgca gcggctggat ggatggcatc aagcctgtcg atctctcccg ggagttcacc
     4801 cgcgaggaga tctccctgcc cctgccctcc gacgatgagc tggccgagat cctcgccatc
     4861 accggcaagt attcgccgga agatgaactc aactgcggcg cctgtggcta tccgacctgc
     4921 cgtgaaaagg cgatcgcggt ctatcagggc atcgcggagg cggggatgtg cctgccctac
     4981 ttgatcgacc agctgcaaaa gaccgagggc gagctgaccg cctccaatgt ggagctgaaa
     5041 aagtcgattg aaaccctgcg ccgaacccag gcgcagctgc tgcagtcgga aaaaatggcc
     5101 tcggtcggcg aactcgccgc cggtgtggcc catgagctca ataatcccct cggcggcatc
     5161 ctcatctaca ccagcctgct gctggaaaag gccgccagcg gggcgcagga gcgggaggat
     5221 ctcgagctga tccgggcgga gaccgagcgc tgcaaaagga tcgtgcgcgg cctcctcgat
     5281 ttttcgcgtc agacacgcat cgaggcggcc attgccgatc tcaaccagat cctgggcggc
     5341 acactcgccc tggtcaccca acaggccctt tttcacaacg tcaaggtgga gcaggacctt
     5401 ctccccggcc tgcccaaggt tttcgtcgac atcggccaga tccagcaggt tctgctcaac
     5461 atcatcctca acgcggtcga ggcgatgcat gaaaagggaa ccctgattat caccacgacc
     5521 tgggatccgg ccaaacagca ggtcttgctg accatccgcg atacgggcac agggatgagc
     5581 ccggaactgc aggcgcggat ctttgaaccc ttttttacaa ccaagccgcg cgggatcggc
     5641 accggactag gcctgtccat cgcctatggc atcatccagc gccaccatgg cgagatcctg
     5701 gtcaggagtg cgccgggcga ggggagtgaa ttcacgatcg tcctgccgga agcggagaaa
     5761 atggtcatga ccgatatcga gctggcaggc attgagaagg atcaggcatg aggagaagca
     5821 gcatggcgga acagggcaaa attttgctgg ttgatgatga ccccgatttc aatatcatca
     5881 accgcaccat cctcgagagc gacggttaca gcgtcgaaga ggccagggac ccggagagcg
     5941 cttgggagaa gatccggagc tggaagccag accttatctg tctcgatgtc atgatgccca
     6001 caggcaccga gggctttcat ttcgcctaca aggtccggcg cgacgaggcc acccggcata
     6061 tccccattct tatgatcacg gcgatccacc agcacagcga ttttcgcttt tccaccgatg
     6121 acggcgagta tctgcccgtc gacgattttg ttgaaaagcc gatcaaacgc gagctgctgc
     6181 tggcgaaggt caaggccctg ctcgctgcca cccggccggc actgcccaaa ccagccgacg
     6241 acaagggcat cggcctgaag aaatagccgc cgccctgcag cgggatgagc cgcccctgga
     6301 gtgcaactgc ccctgacggc cgctgcggtc gcagatttaa catcgtgaga ggcacattca
     6361 ttagaagatt tcttatctgg atgagcgcat ggaaaaaaag aaacgcattc ttttggtcga
     6421 tgacgacccc gactttgtcg agatcaaccg gatgatcctc gaggcctcag actatgaggt
     6481 cgacgcggcc gattcggtcg cccttgccct gcagcagctc gaacaaaata gttacgatct
     6541 cctcatcatc gatctgatga tggaggagcg cgactcgggc ttcactctta cctatgcagt
     6601 gcgcagcgat gagcggctgc gggatctccc catcctcatg ctgacctcgg cccaggaaaa
     6661 gaccgggttc agttttctgc tcagtgagga tcaggagtgg atgaaggtgg atgattttgc
     6721 agccaagccg ctcaagcccg ccgagctggt ggcgaggatc gaaaaactga ccgggaaagg
     6781 cgcgcaagat gcaaagccgt gaggcacgga tcctggtggt ggacgacgag gagatcatcc
     6841 gcatcggctg ccagcgcatt cttcagaatc ccggtttcac catcgatctg gcggagaacg
     6901 gacgagttgg ctgggagaag atccagcaaa agagctatga catggtcctg gtcgacctga
     6961 tgatgccgga gatgggcggg ctcgaggtgc tcgagaaggt ccaggcccat gacgagcaga
     7021 tcatcaccat catcatcacc ggcttcgcca ccatcgaaac cgcggtggat gcgatgcgca
     7081 agggggccta cgactatctg cccaagccct tctctccgga ggaactgcgc accaaaatcc
     7141 gccgcggcct ggaaaaacgc tggctgctgc tggaagcgga ggagct
//
LOCUS       DAOPWU010000169         7177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10432_length_7177_cov_5.169475, whole genome shotgun sequence.
ACCESSION   DAOPWU010000169 DAOPWU010000000
VERSION     DAOPWU010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10432_length_7177_cov_5.169475"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PKM23_09345"
     CDS             <1..1140
                     /locus_tag="PKM23_09345"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY91719.1"
                     /translation="QENLDQIFSPFWGHTFLNINFSQDLLVAAKDFLQQNGTGEIVWI
                     SNRADFPSGTEEARKYAREILALFPDKTILHVLDLENVYSMTYYSDYGYGNTSFTFLS
                     ELTRISGGNLFFYRFHPLKTALAALFFDKVMHYKEVEVQTRMQNGYTFDRQDHTPFEG
                     YYPLDFPVIQTGRLHGEFPMSVTIIANTLETTTRKEFTIPAADVQPGTEHLYKAFYGH
                     LIEKWSRSYQNNWLIGDMIALSLETGLLSPYTAFLVPDNLAPYYVPEFVDETRKGSTP
                     VEPTFTLADSTMQFAAAPNPFNPQTTFYFDLPHRIEGGEYSLSVYNLAGQLVRQWRMA
                     APMKGKHKILWTGDSDAGIPAASGTYIAVLRAGRQIKSLRLTLLK"
     assembly_gap    1198..1297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1298..1884
                     /locus_tag="PKM23_09350"
     CDS             <1298..1884
                     /locus_tag="PKM23_09350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY91720.1"
                     /translation="LLVFMDESHYAQREKGQTMSPEKIHLEKVQETLLLPLWGRFVEN
                     QKQKPLLVDHKAEEIVRNLDYDFGKLEKSVNPLSRAAWIARSIFFDERIIEHLAQYPE
                     ATIINLGCGLDTTCDRVNNGKAHWYEIDFPEVIELRKKFLSEQENRVFIGASALSGEW
                     YKKITARKEVFILMGGVIYYFEEAQVRHLLRAIA"
     gene            1903..2175
                     /locus_tag="PKM23_09355"
     CDS             1903..2175
                     /locus_tag="PKM23_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91721.1"
                     /translation="MAFDYSSKKGIEITNQRVLKEGGMNEGARAKWGIDDIHEIEKWQ
                     PGIKVLENMTMFAEHKKKYPLWKRLGMNISDRMKIMSLARIEIVSA"
     gene            2211..2621
                     /locus_tag="PKM23_09360"
     CDS             2211..2621
                     /locus_tag="PKM23_09360"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HNY91722.1"
                     /translation="MTKPGKLLLLLGMAQALHSMEEIYFHLYDFAAKADVAPPAIISS
                     FMQLEMRSEIFAMMNIVVIAALLAVVPFYEERRRWAVTAAWIAACAEVLNGLYHLTAA
                     MLFARFFPGALSAPLLLLTGALLLHQLAAERKRA"
     gene            3049..4083
                     /locus_tag="PKM23_09365"
                     /pseudo
     CDS             3049..4083
                     /locus_tag="PKM23_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
     gene            4102..4392
                     /locus_tag="PKM23_09370"
     CDS             4102..4392
                     /locus_tag="PKM23_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91723.1"
                     /translation="MQPPPLPVPLEGALLQNYPNPFNPATTISFTIPVPAQVTLRVLD
                     LLGREVDLLLAGHLPAGAYTRQWNAVVIPGGVYYCQLQAGAFRLTRKMVLLK"
     gene            4601..>7177
                     /locus_tag="PKM23_09375"
     CDS             4601..>7177
                     /locus_tag="PKM23_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNY91724.1"
                     /translation="MRPRSILRWFTCVMLMAGAAMPSGRHAHAAGAETPPDARQWSSL
                     PALPDSHGFAGMYAGLCGDALLAAGGANFPDGLPWEGGPKRWNDRIWVLDKPDGDWKP
                     AGRLPSPRGYGVSLTVPGRGVLCAGGSDPFRHFTDVFILSWSPQGDLKTESLPPLPEA
                     MANGCGALIGTTVYIAGGESTPGATSASGRFWSLDLAAVQPQWIALPAWPGPPRTLAA
                     AGVQAGQFYLIGGVALKNGGDGKPARERPYLTDGYAWDPKRQQWKRIADLPFPAAAAP
                     SPMPALAQSHLLLIGADDGAQYGVTASHTGFNSAMLVYHTITDTWTPWGTTEAFPQVT
                     VPVVNWQGRHLLVSGEIRPGVRTPAVRALQLSSRKAAFGWINYATLILYLAAVVWLGT
                     SFSKRNQTTNDFFRGGQSVPWWAAGLSIFATMLSSITFMAIPAQSYSVGWNLILANSY
                     LLLTPLVTLVYLPFYRNLNVTSAYEYLERRFNLATRMIASALFILFQCGRVAIVLYLP
                     ALALATVSRISVTSSILLMGVLCVIYTMEGGIRAVIWTDVAQAIVLMGGAVWALVTII
                     LRVEGGTGTIIRLTNDAGRFLQNVNWDFDLLVAGGWIIMIGSIFSNLFSYTASQDVVQ
                     RYLTTADRKSAARAIWTNAVISPIAQLLFFAIGTALFAFYRLHPGELDPLMPRTDSIF
                     PLFIVRELPAGVAGLIVAGIFAAAQSTLSSSLNSMATAYVTDFHRCLRPQATDAACLK
                     LAKWLTGIIGLAGTGIALVLAQADIRSLWETFLEVIGLFGGTISGLFVLGIFFRRANG
                     AGALAGALASALCVGLVWYNKLTVFWVFPVIGVSVCVLTGWLASLLLPRPAAEADELT
                     LAG"
BASE COUNT         1441 a         2177 c         1968 g         1491 t
ORIGIN      
        1 caggaaaacc tggatcaaat tttcagccca ttttggggcc ataccttcct caatatcaac
       61 ttttcccagg atctgctggt ggcggcgaag gactttctgc agcaaaatgg cacgggtgaa
      121 atcgtgtgga tttccaatcg cgcggatttt ccttccggaa cggaggaagc caggaagtat
      181 gcccgagaga tcctggcgtt gtttcccgac aagaccatcc tgcacgtgct cgacctggag
      241 aatgtttata gcatgaccta ttactcggat tatgggtacg gcaataccag ttttacgttt
      301 ctctccgaac tgacgcgaat atccggtggc aatctctttt tctaccgctt tcaccccctg
      361 aaaaccgccc tggcagcgct ttttttcgac aaggtaatgc attacaagga agttgaagtt
      421 caaacccgga tgcaaaatgg ctataccttt gaccgccagg accatacccc cttcgagggc
      481 tattatcccc tggacttccc ggtgattcag accggcaggc tgcatggcga gttccccatg
      541 tccgtgacga tcattgccaa cacgctggag accacaaccc ggaaggaatt taccattcca
      601 gcagcagatg ttcagcccgg gaccgagcac ctgtacaagg ctttttacgg ccatctgata
      661 gaaaaatggt caagaagtta tcaaaacaat tggctgattg gcgacatgat cgcactcagt
      721 ctggaaacag gcctgctatc cccctacacc gcctttctgg tcccggacaa cctggcgccc
      781 tattatgtgc cggaatttgt ggatgaaacc aggaagggca gcacgccggt tgagccaact
      841 tttaccctgg ctgacagcac gatgcaattc gcggccgcgc ccaatccctt taatccgcag
      901 acgacttttt atttcgatct tccccataga atcgagggcg gtgaatattc tctatctgtg
      961 tataatctcg cgggacaact ggtgagacaa tggcggatgg cggcacctat gaagggcaaa
     1021 cataaaatcc tctggactgg tgattcggat gcaggtatac cggcggctag tggcacctat
     1081 attgccgttt taagagcggg gagacagata aaatcgctgc gattgacgct gctcaagtag
     1141 aatgatgggc aatgttcatc ctgccagtcc cataatattt tggtcgcaca tcttgatnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagc tcctggtatt tatggatgaa
     1321 tcacactatg cacaaagaga aaaaggtcaa acgatgagcc cggaaaaaat ccatctggaa
     1381 aaggtgcagg aaaccctgct gctgccgctg tggggaagat ttgtcgaaaa tcaaaagcaa
     1441 aagccgctgc tggtcgacca taaagcggag gagatcgtca ggaaccttga ttacgatttc
     1501 ggcaaacttg aaaaaagcgt caatcctctc tcacgcgcgg cctggatcgc ccgcagcatc
     1561 ttcttcgatg aaaggatcat tgagcatctg gcgcaatacc ccgaggccac gatcatcaat
     1621 ctcggctgcg gcctggatac gacctgcgac agggtgaaca acggcaaggc acactggtac
     1681 gaaatcgatt ttcccgaggt gatcgagctg cgtaaaaaat ttctgtctga acaagagaac
     1741 agggttttta tcggtgcatc agcgctctcc ggagaatggt acaaaaaaat cacggcgaga
     1801 aaagaggttt ttatcctgat gggcggcgtg atctattatt tcgaagaagc tcaggtcaga
     1861 caccttttgc gcgccatcgc ctaggaattc ggccaagtcg aaatggcctt tgattacagt
     1921 tcgaaaaaag ggatcgagat caccaatcag agagtgctga aagaaggcgg catgaacgaa
     1981 ggggcaaggg cgaaatgggg gatcgatgat atccatgaga tcgagaaatg gcagccgggg
     2041 atcaaggttc ttgaaaacat gaccatgttt gccgagcaca agaagaaata tccgctctgg
     2101 aaaaggttgg ggatgaatat ttcggatcgt atgaagatca tgtccctggc cagaatagag
     2161 atcgtttccg cctgaggact gccttacagt tgtccaatcc cggagctcat atgaccaaac
     2221 ccggcaaact tctccttctc ctcggcatgg cgcaggcgct ccacagcatg gaggagatct
     2281 atttccacct ttacgatttt gccgcaaaag ccgatgtagc acctcccgcc atcatctcga
     2341 gctttatgca gctggagatg aggtctgaga tctttgcgat gatgaatatc gtcgtcattg
     2401 cagctcttct ggctgtggtt cccttttatg aggagaggcg ccgctgggcc gtgacagcgg
     2461 cgtggattgc agcctgcgcc gaggtattga acggacttta ccatctgacg gccgcgatgc
     2521 tctttgcgcg cttcttcccc ggcgcccttt cagcccccct gctgctgtta acgggagcgc
     2581 tcctgctcca ccagcttgct gcggagagga agcgagcttg accattgccg gaagcaggca
     2641 gctccggagt tcctccagtc tattcaagag tgtcttcatc cttgtccgat cctgattcct
     2701 gtgcaagtta ttctcgccgc gatgccgcct gcactattcc tgatacaaat tgtgcttgat
     2761 tatcagaggc tgattggcta atttaccggc attcaattag cgatgagggt accggcaaga
     2821 ggccccggct cagacgggct gccgggaggt ccggcagccc tgcaacgccg catggagtca
     2881 tcattccagg aagaaaatgc aatgagaaag ttgctctata tcgccaggcc gcttgcgtgc
     2941 atcgttctga tctgtacacc ggcttttgcc gccggcgacg cagcaggcgg ccttacggcc
     3001 ccggccatgg ccgacagcat cccgccggac cacacggata tgcgcgatat cagttcctac
     3061 gccctgtctc aggagatggt gccgggatgg aatcttggca actcccttga agcgatcggt
     3121 ggcgagaccg catggggcaa tcccaaggtg acacagcggc ttatcgattc ggtgaaagca
     3181 gccggtttca aatcggtgcg catccccgtc gcctggagcc ggttcacgga tcaggccagc
     3241 tataccatcg atcccctctg gctcaaccgc gtcgaggatg tagtgaatta cgtcctcagt
     3301 gcaggcatgt atgccatgat caatgaacat tgggacaacg gctggatcat ccccaccaat
     3361 gaccagcagg aggaagtgaa caaacggctg gcggccatgt ggcggcagat tgccgtgcgc
     3421 ttccgggact acgatgacta tctcattttc gcgggcacca atgaggtgca tgtggattac
     3481 aatgcaccca ccagcgaata ttatacggtc cagaatggct ataatcagac ctttgtcaat
     3541 gcggtgcgtt cgaccggcgg ccgcaacacc taccgctatc tggccgtgca gggatacaac
     3601 accaatatcg attacaccaa ccgctttttt gtcatgccgg ccgatgccca gcagtcgcgg
     3661 ctgctggtgg aggtgcacta ttacgatccc tacaacttta ccattaactc cagcagcacc
     3721 atcacccaat ggggcaagaa cgcaaccgat cctgcgagga ccgagacctg ggcgaatgag
     3781 gcctgggcgg atgcccagtt caagaaaatg aagattaaat tcatcgacaa gggattcggc
     3841 gttcttctgg gcgagtatgg cgtccaggcg cggctgaatc tgggcagcac cgcgcgcaac
     3901 caggagcatg ccgcctaccg gctctactgg atgcagtaca tcacgcgctc tctggagcgg
     3961 cacggtctgg tgccgatgta ctgggacaac ggccccaccg gcaacaacag catgggcctt
     4021 ttcaatcgcg ccaccggggc tcaggtttat cgcgatatta tcaaggcgat tgtcgacacc
     4081 tcgagcctga acaccagcag cgtgcagccg ccgcctctcc cggttcctct cgagggcgct
     4141 cttttgcaga actatcccaa tcccttcaat ccggcgacga ccatctcctt caccataccg
     4201 gttccggcgc aggtgacgct gcgggtgctt gatctgctgg gaagagaggt ggacctcctg
     4261 ctcgcaggcc acctgccggc aggcgcctat acccgccagt ggaacgcagt tgttatccct
     4321 ggcggggtat attactgcca actgcaggcc ggcgctttcc gcttaacccg caagatggtc
     4381 ctgctcaagt gaactcctgc cgggcgtgtg accctgcatg ccgctcccac cctgcggtga
     4441 tccctctgtt catctgcatc ggctctctta ccggtacaat cagcctaaaa atcagggagt
     4501 ataggatgaa gcattcttac gcattggttc tcgcactgct gggcttgtac ccgtccagtt
     4561 tgttctgatc actatattga ctgcagagga agtccagcag atgagaccga gatcgatcct
     4621 gcgatggttc acctgcgtga tgctgatggc cggagcggca atgccttccg gccggcacgc
     4681 ccatgcggcc ggcgccgaga ctccgcctga cgcccggcag tggagcagcc ttccggccct
     4741 gccggactcc catggctttg cggggatgta cgccgggctc tgcggcgatg ccctgctggc
     4801 tgccggcgga gccaattttc cggatggact gccctgggag ggaggcccga aaaggtggaa
     4861 cgaccgcatc tgggttctgg ataagccaga tggagactgg aagcctgccg gccgcctgcc
     4921 ctcgccgcgc ggctacggcg tcagcctcac agtgccggga cgcggcgtgc tctgcgccgg
     4981 cggcagtgac cccttccgcc attttactga tgttttcatc ctcagctgga gcccgcaagg
     5041 ggatttgaag accgagtccc tgccgcccct gccggaagcg atggccaatg gctgcggcgc
     5101 gctcatcggt acgacggtct atatagccgg cggagaaagc actcccggcg cgacctccgc
     5161 cagcggccgc ttctggagcc tcgacctggc ggccgtgcag ccgcagtgga tcgcgctgcc
     5221 ggcctggccc ggccctccgc gcaccctcgc cgcggcaggc gttcaggccg gccaattcta
     5281 tctcattggc ggcgtcgcat tgaaaaacgg cggcgatggc aagccggcgc gcgagcggcc
     5341 ctacctgacc gacggctacg cctgggatcc caaacggcag caatggaaac ggatcgccga
     5401 cctgcccttt ccggccgcag ccgcgccttc gccgatgccg gcgctggccc agtcccatct
     5461 cctgctcatc ggcgccgatg acggcgcgca gtacggcgtg accgccagcc acaccggctt
     5521 caattccgcc atgctggtct accacaccat caccgacacc tggacgccct ggggcacaac
     5581 tgaagccttc ccgcaggtca cggttccggt cgtcaactgg cagggccggc accttctggt
     5641 cagcggcgag atccgtcccg gtgtgcgcac ccccgctgtg cgcgcgctcc agctctcctc
     5701 ccgcaaggcg gccttcggct ggatcaacta tgccaccctc atcctctacc tggcggcggt
     5761 ggtctggctg ggtaccagct tcagcaagcg caaccaaacc accaatgact ttttccgcgg
     5821 tggccagagc gtaccgtggt gggccgccgg actgagcatc tttgcgacca tgctcagctc
     5881 gatcaccttc atggccatcc ccgcccaatc ctacagtgtc ggctggaatc tcatcctcgc
     5941 caacagctac ctgctgctga cgcccctggt gaccctggtc tatctgccct tttatcgcaa
     6001 cctcaacgtc acctccgcct acgaatacct ggagcggcgc ttcaacctcg ccacgcgcat
     6061 gatcgccagc gcccttttca ttctcttcca gtgcgggcgc gtggccatcg tgctctacct
     6121 gcccgccctg gccctggcga cggtcagccg catcagcgtg accagctcca ttctgctcat
     6181 gggagtcttg tgcgtgatct acaccatgga gggcggcatc cgcgccgtga tctggaccga
     6241 cgtcgcgcag gcgatcgtgc tcatgggggg cgcggtctgg gccctggtga ccatcatcct
     6301 gcgcgtggag ggcggcacgg ggacgatcat ccggctgacc aatgacgccg ggcgcttcct
     6361 gcagaatgtg aactgggatt tcgacctcct cgtcgccggc ggctggatca tcatgatcgg
     6421 ctctatcttc tccaacctct tctcctacac cgcgagccag gatgtcgtcc agcgctacct
     6481 caccaccgcg gaccgcaaga gcgccgcgcg tgcgatctgg accaacgccg tgatctcgcc
     6541 catcgcccag ctcctcttct tcgcgatcgg gaccgccctc ttcgcctttt accggctgca
     6601 tcccggcgag ctcgaccccc tgatgccgcg gacggacagc atctttccgc tcttcatcgt
     6661 cagggaactg cccgccggcg tggcgggcct gatcgtggcc ggcatcttcg ccgccgcgca
     6721 gtcgaccctc tccagcagcc tcaacagcat ggccaccgct tatgtaaccg atttccaccg
     6781 ctgtctgcgc ccgcaggcca ccgacgccgc ctgtctcaaa ctcgccaaat ggctgaccgg
     6841 catcatcggg ctcgcgggca cgggcatcgc cctggtgttg gcgcaggccg acatccgttc
     6901 gctctgggag accttcctcg aggtcatcgg tctctttggc ggcacgatct caggactctt
     6961 cgtgctcggc atctttttcc gccgcgccaa cggcgccgga gcgctcgccg gcgccctggc
     7021 cagcgctctc tgcgtcggac tggtttggta caacaaactg actgtcttct gggttttccc
     7081 cgtcatcggc gtcagcgtct gcgtcctcac cggctggctg gccagtctgc tgctgccgcg
     7141 cccggccgcg gaggcggatg aactcaccct ggccggt
//
LOCUS       DAOPWU010000170         7059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10640_length_7059_cov_4.259138, whole genome shotgun sequence.
ACCESSION   DAOPWU010000170 DAOPWU010000000
VERSION     DAOPWU010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10640_length_7059_cov_4.259138"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1009
                     /locus_tag="PKM23_09380"
     CDS             194..1009
                     /locus_tag="PKM23_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNY91725.1"
                     /translation="MFNYESYLRLDKFPLIWCSGCGDGIVLKSMIRAIDKLGLSKDDV
                     CMVSGIGCSSRLTGYVDFNTLHTTHGRALTFATGIKMAKPRLHVIVVTGDGDATAIGG
                     NHFIHAARRNIDITVILFNNFIYGMTGGQVSPCTPKGKRASTAPYGNFENAFNISGLA
                     QAAGASFVARATVYNAVQLEKLIQRAIEKKGFSLVEVLSPCPTAYGRRNREGTPVDMI
                     LQQKETTVSVAAAEKMSAEELEGKIITGVLTDVERPECREEYQKLIDSFRKEG"
     gene            1015..1566
                     /locus_tag="PKM23_09385"
     CDS             1015..1566
                     /locus_tag="PKM23_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNY91726.1"
                     /translation="MSFRYEIRLSGEGGQGLVLAGKILAEAAAVYDDLNATQSQSYGP
                     EARGGASRSEVIISDDEIDYPKAVSIDLLLALTQESCDRYYKDLKKGGILLVDSDAVN
                     RLPEGEFRVISAPIIGLARERVGRALVANIVALGFIAGISGIVSEKSLRAAIEARVPK
                     GTEELNLTAFATGLAEARLLMKN"
     gene            1606..2031
                     /gene="ndk"
                     /locus_tag="PKM23_09390"
     CDS             1606..2031
                     /gene="ndk"
                     /locus_tag="PKM23_09390"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNY91727.1"
                     /translation="MQRTLAICKPDCLARSLAGQVLSRIEAGGFRIVAMKMIRLTRSQ
                     AGHFYDVHREKPFYPELLDFMTEGPVVVAVLEKGDAVESWRALMGATNPAQAAEGTIR
                     RDLAESMSRNVVHGSDSPENARREIAFFFSESELLAAEK"
     gene            2116..2625
                     /locus_tag="PKM23_09395"
     CDS             2116..2625
                     /locus_tag="PKM23_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNY91728.1"
                     /translation="MQALNIANMAPGHYEETRQASAADLDLHERDEFQAPITMVIRID
                     KASDELIVTVDWQTVISGSCDRCADPLQIPLSDALAMVYTYDPQERGEEDEDVLLISE
                     STREVDLTDHLRQSLILALPLKLLCREECKGLCSHCGANLNNESCSCPPDPLDPRWEK
                     LEQLLNRKK"
     gene            2628..2810
                     /gene="rpmF"
                     /locus_tag="PKM23_09400"
     CDS             2628..2810
                     /gene="rpmF"
                     /locus_tag="PKM23_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNY91729.1"
                     /translation="MPLPKRRHSSARRDKRRANWKAATPTVTECSNCHQPKLSHRACP
                     SCGYYKSRSVFLPKEA"
     gene            2904..3917
                     /gene="plsX"
                     /locus_tag="PKM23_09405"
     CDS             2904..3917
                     /gene="plsX"
                     /locus_tag="PKM23_09405"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNY91730.1"
                     /translation="MIKIALDAMGGDNAPHDVVHGGVEAARAAKGGLEVVMVGDEETV
                     HQLLARHFHIHELAISVVHASQSIAMDESPAAAVKQKPDASVSVAMRLHKEGKVDGVV
                     SAGNTGALMTSALFNLKRMEGIRRPAISTLMPTEKGRMLLLDVGANVDNKPLDLVQFA
                     VMGSTYYSHLFDEPNPTVGLLNIGHEAMKGNEMSLQAHELLSASSVNFIGNVEGGDVL
                     RGSANVVVCDGFVGNIVLKFGESLLGMFNSSLRRISRKYIFSQLGAVMMKPTFDGIRK
                     IFDYQEYGGAPLLGVQGICFKAHGRSTPRAIRNAVLATKKMVDGHVNQRIRTEIGKLA
                     FSE"
     gene            3971..4939
                     /locus_tag="PKM23_09410"
     CDS             3971..4939
                     /locus_tag="PKM23_09410"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNY91731.1"
                     /translation="MITGTGCAVPGRVLTNHDLEKMVETSDEWIRERTGISERHIIEE
                     GRYTSDLCAEAARGALAEAGLSPLDVDMICVATVTGDMGFPSTACIVQEKIGAHNGAA
                     FDIAAACSGFLYGLSWADAMIATGRVRTVLVIGGETLSRITDYQDRTTAVLFGDGAGA
                     AVLQPSDGRRGILGSYLKSDGRFSRLLYLDGMGTQYPPTHESIDRRAGYIRMEGREVF
                     KQAVTAMGDAAETVLQRAGLTGEDVDLLIPHQANMRIIDATARRVKVPPERVFVNVDR
                     YGNTSAASIPIAIDEARRSDRLGPGKLALLVAFGSGLTWASALVRF"
     gene            4940..5860
                     /gene="fabD"
                     /locus_tag="PKM23_09415"
     CDS             4940..5860
                     /gene="fabD"
                     /locus_tag="PKM23_09415"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNY91732.1"
                     /translation="MGRRAFIFPGQGSQEVGMGRDLYEAYPSVRALYIRANELLGFDL
                     ARLSFEGPPDELRRTQVTQPALFVHSYALQGLLRRRGIRPRMAAGHSLGEFTAFASAG
                     ALSFAEGMRLVARRAAAMTAAAERQPGAMAAIIGLDQVLLEKICTALPTGENAVVANI
                     NSDQQLIVSGSKVGVETVIKAAVAAGARRALPLAVSGAFHSPLMEPARAGLAQALAEA
                     PFRAPRMPVYCNVTAQPAQTSGEIRSLLERQLVSPVRWSEQVRRMAADGADEFIEIGA
                     GSVLSGLVKRIAPEVRVRTVSTLKDVLEYV"
     gene            5853..6614
                     /gene="fabG"
                     /locus_tag="PKM23_09420"
     CDS             5853..6614
                     /gene="fabG"
                     /locus_tag="PKM23_09420"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNY91733.1"
                     /translation="MYEDGQVIDLTGKTAVVTGGARGIGKAIVLRLAGAGADVVVSDI
                     DGAGAEATAAEVKALGRRSLAVQGDISSMSDAEALIEAAMAAMGRVDIFVNNAGITRD
                     GLLVRMDEKQWDSVIAVNLRGTFNGTRAAAKVMMRQRAGKIINIASVVGLMGNVGQAN
                     YAASKAGVIGLTKSAAKELGGRNVQVNAVAPGYIETDMTRELPQEAKEGFLALIPLKR
                     PGTPGDVADIVLFLASPLADYVTGQVVQVDGGMVM"
     gene            6712..6951
                     /locus_tag="PKM23_09425"
     CDS             6712..6951
                     /locus_tag="PKM23_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNY91734.1"
                     /translation="MAIEDRVKEIIVQQLGVAASEVVPEASFIDDLGADSLDTVELVM
                     AFEEEFGIEIPDEEAEKLASVGDALKYLNEKLGKN"
BASE COUNT         1417 a         2091 c         2212 g         1339 t
ORIGIN      
        1 cgcatggccc gcactgcccg gcgcattatt gtgcccgaga tgaacctggg gcagatcgcc
       61 catgaggttg agtgggccct ggccggcagc gcgccggtga cgcgcctgac caaagtcaac
      121 ggcgatccga tcagcccgcc ggaaatttac cagatcatcc ccggttgagt cgagagagaa
      181 ggagagaggc ccgatgttca actatgaaag ctatctgcgg cttgataaat tccccctcat
      241 ctggtgttcc gggtgcgggg atggcatagt gctcaagtcg atgatccgcg ccatcgacaa
      301 gcttggcctg agcaaggatg atgtctgcat ggtgtcgggc atcggctgct cgagtcgtct
      361 gaccggctat gtcgatttca ataccctgca caccacacac gggcgcgccc tgacctttgc
      421 caccggcatc aaaatggcca agccccgcct ccacgtcatc gtggtgaccg gcgacggcga
      481 cgccacagcg atcggcggca atcattttat ccatgcggcg cggcgcaata tcgacatcac
      541 cgtgatcctg ttcaacaact ttatctacgg catgaccgga gggcaggtct cgccctgtac
      601 gcccaagggc aagcgcgcca gcaccgcgcc ctatggcaat tttgagaacg ccttcaatat
      661 cagcgggctg gcccaggccg ccggcgccag ctttgttgcc cgcgccactg tttacaacgc
      721 tgtccagctc gaaaagctca ttcagcgggc catcgagaag aagggcttct ccctggtcga
      781 ggtgctctcg ccctgcccga ccgcctacgg gcggcgcaac cgcgaaggca cccccgtgga
      841 catgatcctg cagcagaagg agaccacggt cagcgtggct gcagccgaga agatgagcgc
      901 ggaagagctc gagggcaaga tcatcaccgg cgttctcacc gatgtcgagc gccccgagtg
      961 ccgcgaagag tatcaaaagc tgatcgattc attcaggaag gagggctgag cgcgatgagt
     1021 tttcgctatg aaatccgctt gagtggcgag ggcggacagg gactggtgct ggccggcaag
     1081 atactcgccg aagcggcggc ggtctacgat gacctcaacg ccacccagag ccagagctac
     1141 ggcccggaag cccgcggcgg cgccagccgc tccgaggtga tcatctccga cgacgagatc
     1201 gactatccca aagcggtcag catcgacctt ctgctggcct tgacccagga gtcctgcgac
     1261 cgctactaca aggatctcaa gaaggggggg attctgctgg tggacagcga tgcagtgaac
     1321 cgtctgcccg aaggggaatt ccgcgtgatc agcgcgccca tcatcgggct ggcccgggag
     1381 cgggtcggcc gggccctggt ggccaacatc gtcgctttgg gcttcatcgc cggcatttcg
     1441 ggcatcgttt cggaaaagtc gctgcgggcg gcgatcgagg cgcgcgtccc gaaggggacc
     1501 gaagagctca acctgaccgc ctttgccacc gggctggcag aggcgcgtct gctcatgaaa
     1561 aactagtccg taacgcccgt catctcttca cgatacagga ggaccatgca gagaacgctt
     1621 gccatttgca aacccgattg tctggcccgc tcgcttgcag gccaggtgct gagccgtatt
     1681 gaagcagggg gatttcgcat cgtcgcgatg aaaatgatcc ggctgacccg gagtcaggcg
     1741 gggcattttt atgacgtcca tcgcgaaaag cccttctatc ccgagctgct cgattttatg
     1801 accgaaggtc ctgtggtggt tgcggtgctc gagaagggtg atgcggtcga gagctggaga
     1861 gcgctgatgg gcgccaccaa tcccgcgcag gcggcggagg ggacgatccg tcgtgatctg
     1921 gccgagagca tgtcgcgcaa tgtggtgcac ggctcggatt cgcctgaaaa tgcccgccgc
     1981 gagatcgcct ttttcttcag tgagagcgaa ctgctggctg cagaaaaatg aatataattc
     2041 agaaaatcgc gatttttagc ttgactatgt gacgctttat tgctaaattt tccctcgatt
     2101 tttggaacga aaaaagtgca agctctcaat atcgccaata tggccccggg gcattatgaa
     2161 gagacccggc aggcctcagc cgcggatctc gacctgcacg agcgggatga gttccaggcg
     2221 cccataacca tggtcatccg gatcgacaag gcctccgatg aactcatcgt gactgtcgac
     2281 tggcagactg tgatctccgg cagctgcgac cgctgtgcgg atcctcttca aatcccgctg
     2341 agcgacgccc ttgcaatggt ctacacctac gatccgcaag agcgcgggga ggaggacgag
     2401 gatgtgctcc tcatcagcga atcgacccga gaggtcgatc tgaccgacca cctccgccag
     2461 agtctgatcc tggcgctgcc tctcaaactg ctttgccggg aggaatgcaa gggattgtgc
     2521 agccactgcg gcgccaatct caacaacgaa tcatgtagtt gcccgccgga tccgctagat
     2581 ccgcgctggg aaaagcttga acagctccta aacagaaaga agtagccatg ccattaccca
     2641 aaagaagaca ctccagcgcg cgccgcgaca agagacgcgc caactggaaa gcagccacac
     2701 cgacggtcac cgagtgctcg aattgccatc agcccaaact ctcccaccgg gcatgcccca
     2761 gctgtggcta ttataaaagc cgttcggttt ttttgccgaa agaggcttaa gaccaatttc
     2821 tttgcccacc aagcctgacg tgacctcagc aatcaaagcg attgttgagg tcacctctgt
     2881 ttgaactcgt tccggagttg aaattgatca agatcgcgtt agatgccatg ggaggggaca
     2941 atgcccctca tgatgtggtg catggaggcg tcgaggctgc ccgcgccgcc aagggcggtc
     3001 tcgaggttgt catggtggga gatgaagaga cggtgcacca gcttctggca cggcattttc
     3061 acatccatga actggcgatt tccgtcgtcc atgcctcgca gtcgattgcc atggacgagt
     3121 ctcccgcggc tgcggtcaag cagaagccag acgcctcggt gagtgtggcc atgcggttgc
     3181 acaaggaggg caaggtggat ggcgtggtca gcgccggcaa taccggagcc ctgatgacct
     3241 cggccctttt caatctcaag cgcatggagg ggatccgccg gcccgccatc agtaccctca
     3301 tgcctaccga aaaggggcgg atgctgctgc tcgatgtcgg cgccaatgtc gacaacaaac
     3361 cgctagatct ggtccagttc gccgttatgg ggtccaccta ttacagccat cttttcgacg
     3421 agccgaatcc gactgtcggg ctgctcaata tcgggcatga ggcgatgaag ggcaacgaga
     3481 tgagcctgca ggcccatgag ctgctttccg cctccagcgt caatttcatc ggcaatgttg
     3541 aggggggcga tgtcctgcgt ggatcggcca acgtcgtggt ctgcgacggc tttgttggca
     3601 atatcgtgct caaattcggc gaaagcctgc tgggaatgtt caactcgagc ctgcgccgga
     3661 tcagccgcaa gtatattttt tcccagctcg gggcggtgat gatgaagccg accttcgatg
     3721 gcattcgcaa gattttcgat taccaggaat acggcggcgc acccctgctc ggggtgcagg
     3781 gcatctgttt caaggcccac gggcggtcga ccccgcgcgc catccgcaac gctgttctgg
     3841 cgaccaaaaa gatggtcgat ggccatgtca accagcgcat ccggacggaa atcggcaagc
     3901 tggccttttc cgaatgatcg tctgacccta tccgaggata ctgcgattga atagtccgct
     3961 gctccatgcc atgatcaccg gtaccgggtg cgctgttccg gggcgtgtgc ttacgaacca
     4021 cgacctggaa aagatggttg aaaccagcga cgagtggatc cgtgagcgca ccggcatctc
     4081 cgagcgccac atcatcgagg aggggcgtta cacctccgat ctctgcgccg aggcggccag
     4141 gggcgccctc gccgaggccg gcctttcgcc cctggatgtg gatatgatct gcgtggccac
     4201 cgtcacgggt gatatgggat ttccctccac cgcctgtatc gtgcaggaaa agatcggcgc
     4261 ccacaatgga gccgccttcg acatcgctgc agcctgttcg gggtttctct acggcctctc
     4321 ctgggcggat gcgatgatcg ccaccggcag ggtgcgcaca gtgctggtca tcggcggaga
     4381 gaccctctcc cgcatcaccg actatcagga ccgcaccact gccgttcttt tcggcgacgg
     4441 cgccggggcg gcggtgctcc agccctcgga cggccggcgc ggcatcctcg gcagctatct
     4501 caagagcgac ggccgtttca gccggctgct ctatctcgac ggcatgggca cccagtaccc
     4561 gcccacccat gagtcgatcg accggcgtgc tggctatatc cgtatggagg ggcgcgaggt
     4621 tttcaaacag gcggtgacgg ccatggggga tgctgcggag acggttcttc agcgcgccgg
     4681 actcaccggt gaggatgtcg atctgctcat tccgcaccag gccaacatgc gcatcatcga
     4741 tgccacggcc aggcgggtga aagtcccgcc cgagcgggtc tttgtcaatg tcgatcgcta
     4801 cggcaatacc tcggccgcct ccattcccat tgccatcgac gaagcgcgcc ggagcgaccg
     4861 gctcgggccg ggcaagctcg ccctgctggt cgcctttggt tccggattga cctgggcgtc
     4921 tgccctggtg cgtttttaga tgggcagaag ggcctttatc tttcccggcc agggttcaca
     4981 ggaagtgggc atgggcaggg atctttatga ggcctaccct tcggtgcggg ctctttatat
     5041 cagggctaac gaactgctcg gtttcgatct cgcccgcctc tcctttgagg ggcctcccga
     5101 cgagctgcgc cgaacccagg tcacccagcc tgccctcttc gtccacagct atgccctgca
     5161 ggggctgctg cgcaggcgcg ggatcaggcc gaggatggcc gctggtcaca gcctcggcga
     5221 gttcaccgct tttgcgtcgg ccggagcctt gagttttgcc gaggggatgc gcttggtggc
     5281 caggcgcgcc gcggcgatga ccgctgccgc cgaacggcag cctggcgcca tggctgcgat
     5341 tatcggcctc gaccaggtgc tgcttgaaaa aatttgcact gcgctcccca cgggggagaa
     5401 tgcggtcgtc gccaacatca actcggatca gcagctcatt gtctccggca gcaaggtggg
     5461 cgttgagacg gtcatcaagg cggccgtggc ggcaggcgcc cggcgggcgc tgcccctggc
     5521 tgtgagcggg gcctttcatt cgcccctgat ggaacctgcg cgcgccggac tggcgcaggc
     5581 tctggccgaa gctcctttcc gggcgccgcg catgccggtg tactgcaatg tgactgcgca
     5641 gcccgcgcaa acgagtggtg aaatccgctc cctgctggag cggcagctgg tcagccccgt
     5701 gcgctggtct gagcaggtgc gccggatggc agcagacgga gccgatgagt tcatcgagat
     5761 cggggccggg agcgtactca gcggcctggt gaaacgcatc gcacccgagg tccgggtgcg
     5821 tacagtcagc accctcaagg atgtgctgga atatgtatga ggatggacag gtgatcgatc
     5881 tgacaggaaa aacagcggta gtgaccggcg gagcgcgggg catcggcaag gccatcgtcc
     5941 tgcgtctggc cggcgccgga gccgacgtcg tggtctcgga catcgacggt gcgggcgcgg
     6001 aggcgaccgc cgcagaggtg aaggccctcg gccgccgctc cctggccgtg cagggcgaca
     6061 tcagttcgat gagtgacgct gaggccctga ttgaagccgc gatggcagcc atgggacggg
     6121 tcgacatctt tgtcaacaat gccggtatca cccgcgacgg tctgctcgtg cgcatggacg
     6181 agaagcagtg ggacagcgtc atcgccgtca atctgcgcgg gaccttcaac gggaccaggg
     6241 cggcggcgaa ggtgatgatg cggcagcgcg ccggcaagat catcaatatt gcctcggtag
     6301 tgggattgat ggggaatgtc ggccaggcca actatgccgc ctccaaggcg ggtgtgatcg
     6361 gcctgaccaa gtcggcagcc aaggaactgg gcggacgcaa cgtccaggtc aacgccgtgg
     6421 ccccgggcta tatcgaaacc gacatgacca gggaactgcc acaggaggcg aaggagggtt
     6481 ttctcgccct cattcccctc aagcgccccg gaaccccggg agatgtggcg gatatcgttc
     6541 tcttcctggc cagcccgctt gccgattatg tgaccggcca ggtggtgcag gtcgatggcg
     6601 ggatggtcat gtaggagaga taggttcgag agcaagagac aagcaggcag aatatacata
     6661 gaccataata acaagcagca cattttccaa accccatagg aggaacaaac aatggctatt
     6721 gaagatcgcg ttaaagaaat catcgtccag cagcttggcg tcgccgccag cgaggtcgtc
     6781 cctgaagcat ccttcatcga tgatctgggg gccgattccc tggataccgt cgagctggtc
     6841 atggctttcg aggaagagtt tggcatcgaa atccccgacg aggaggccga gaagctcgcc
     6901 tctgtcggtg atgccctcaa gtacctcaat gagaagctgg gcaagaatta acacaccaga
     6961 ggggcagcgc aagttgcccc tcatttttag attccgccgg caagggctta aaatacaagc
     7021 ttgttgaggt gagcgatgga tggcagaaga gttgtggtg
//
LOCUS       DAOPWU010000171         7026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10696_length_7026_cov_6.304834, whole genome shotgun sequence.
ACCESSION   DAOPWU010000171 DAOPWU010000000
VERSION     DAOPWU010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_7026_cov_6.304834"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PKM23_09430"
     CDS             <1..739
                     /locus_tag="PKM23_09430"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNY91735.1"
                     /translation="RDIQSLEALPPGKVDPDVASYIRRGYEKSAILNCIALLGWNPGE
                     GDDRQVFSLDELVQEFSLGRINKSAAIFDLKKLNWLNKEHIMRRSPESIAAAVRAMPE
                     AEALGEIDQAYLLGVVGLLHERLHFIGDFWRMAGYFFKDPESYEAEALQKRWSPETAP
                     LLQELSREFEAAGVFAAAEIEQALRTVAERHDLKGSLLIHPLRLAVTGLSVGPGLFEL
                     MELLGRETVLRRLTLAIKQVPMILGIG"
     gene            741..2480
                     /locus_tag="PKM23_09435"
     CDS             741..2480
                     /locus_tag="PKM23_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNY91736.1"
                     /translation="MENVNGQAERTEQGSGEAAVSTAPPNFIRTIIREDLKTGKYQGR
                     VHTCFPPEPNGYLHIGHAKSICLNFGLAREFGGKTNLRYDDTNPAKEDVEYVESIEED
                     VRWLGFDWEGRKFYASDYFEQLYEYAVQLIKLGMAYVCDLSPDEIREYRGTLTEPGKN
                     SPYRDRSVAENLDLFERMRKGEFAEGSRVLRARIDMASPNLNMRDPAIYRIKKVEHHR
                     TGDTWCIYPMYDYAHCISDSIEGITHSICTLEFEDHRPLYDWFLDQLGIYHPQQIEFA
                     RLNLSYTLLSKRKLLQLVTEKHVSGWDDPRMPTITGIRRRGYTPESIRTFAEIIGVAK
                     RDSVVDIQLLEHCLREDLNQRAPRRMAVLNPLRVVIDNYPEGEFEELDAVNNPEDPAM
                     GTRKVPFGRELYIERDDFREDPPKQFHRLSPGREVRLRYAYFVTCTGLEKNDQGEVTT
                     LHCSYDPASRGGGTPDGRKVKGTIHWVSAAHAFNATVRLYDSLFTRPDPQATTEGESF
                     LDYLNPDSLRVLTGCKLEPSLAEAEPGARLQFERNGYFVADSVDSKPGVPVFNRTVAL
                     RDSWAKIEKKIGG"
     gene            2615..2685
                     /locus_tag="PKM23_09440"
     tRNA            2615..2685
                     /locus_tag="PKM23_09440"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2647..2649,aa:Gln,seq:ctg)
     gene            2924..>3123
                     /locus_tag="PKM23_09445"
     CDS             2924..>3123
                     /locus_tag="PKM23_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91737.1"
                     /translation="MYLAKWTHIALTLCAALMILVVHLKRTGTETPSGGIAMVPLAEI
                     TAGPAPVFSINDPSPQPVITAA"
     assembly_gap    3124..3223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3452..4165
                     /locus_tag="PKM23_09450"
     CDS             3452..4165
                     /locus_tag="PKM23_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91738.1"
                     /translation="MESRSASGEEVHRQVVALIKRYIEEQHGREARPVDLASASRMAP
                     LGMSARVVTSPGTGRWVLGASAGRQSLAGSVLCSELYWGRRLSSGLQIGFNLSTSLVK
                     GVSGESGGGTTLLQGDLQMRLFLQPRAKLAPFVLLGAGYGEYREEESSSAAPVAIAGA
                     GLEYRFSRRLGAQVTASYEKMQVKKQLPESGGSGRGVWELKAGLVLAIGGPRMIAGPV
                     SSGLAAAEFSAEQAEHLPR"
     gene            4353..4426
                     /locus_tag="PKM23_09455"
     tRNA            4353..4426
                     /locus_tag="PKM23_09455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4387..4389,aa:Arg,seq:tcg)
     gene            complement(4477..5187)
                     /locus_tag="PKM23_09460"
     CDS             complement(4477..5187)
                     /locus_tag="PKM23_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91739.1"
                     /translation="MLKEEIIKRSPIRILEKSIHGGLGKGNLGVFVARKGVGKTACLV
                     HFATDNLLRGQKALHISFSDDPQHIEHWYTQVFHEIARAYKLAGAMDIYEEIIRNRLI
                     LHFKRSGLNLDSVKSSIVRLIKDSAFVPQIIIVDGFSFDKASESDLHFWKKLAQEHDA
                     EIWFSATLPPELPITDQTIIPAPINAFAETLTVVILLVAHRDYIELKLVKDNGERQLD
                     RLQLRLDPKTLLIANRRI"
     gene            complement(5320..>7026)
                     /gene="fusA"
                     /locus_tag="PKM23_09465"
     CDS             complement(5320..>7026)
                     /gene="fusA"
                     /locus_tag="PKM23_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNY91740.1"
                     /translation="RYKVPRLAFINKCDRMGADPERIVEQLRDKLGHNAVLMQLPIGL
                     EANFEAIIDLIRMKAIYFDGSNGEILREEEVPAGLLEKALEKRDLLIDRASLFSDALA
                     EAYLEDRVTEELIYQAVRQGTLALELTPVFIGSAFKNKGIQTLLDGVVDYLPAPTEIE
                     NRALDLDAGETEILLQPDNALPAVLLAFKLDDTRFGQLTYVRIYQGMIRKGDELRNSR
                     SQKKVRIGRLIRMHADEMEDINEASAGDIVALFGIDCASGDTFCSTDINYSMTSMFVP
                     SPVISLSIRPLDNKSGENMAKALNRFSKEDPTFKTFVDPETGESIIRGMGELHLDVYV
                     ERMRREYGVALETGAPQVAYRESISQAASFDYTHKKQTGGAGQYARICGQVEPFHEGV
                     FEFVDEIKGGVIPVEYVPSCKKGFEAALEKGPLVGFPIVGVRVIVNDGNYHPVDSSDM
                     AFQTAASSAFREFYSRCKPIILEPVMRVAVETPPEFQGAVLATINQRRGMILSATEDK
                     TFTAVEAEVPLGEMFGYSTVLRSATQGKAEFTMEFKRYAKVPESIAEELKKAYQEKLK
                     NR"
BASE COUNT         1364 a         1922 c         2155 g         1485 t
ORIGIN      
        1 acgggacatc cagtccctgg aggctttgcc ccccggcaag gtcgatcccg atgtcgcctc
       61 ctatatccgc agggggtatg aaaagtcggc catcctcaac tgcattgcgc tgctcggctg
      121 gaatcccggg gagggggatg accgtcaggt tttttccctc gatgagctgg tgcaggagtt
      181 ttctctgggc cgcattaaca agtcggcggc gatttttgat cttaaaaagc tcaactggct
      241 caacaaggag cacatcatga ggcgctcccc cgagtcgatc gcggctgctg tgcgtgccat
      301 gcccgaggcg gaggccctgg gagagatcga tcaggcctat ctgctcgggg ttgtcgggct
      361 gctgcatgag cgcctccatt tcatcggcga tttctggagg atggccggct acttcttcaa
      421 ggatccggag agctatgagg cggaagccct gcagaagcgc tggagcccgg aaaccgctcc
      481 gttgcttcag gaactgagcc gcgagttcga agccgccggg gtttttgccg cggcggagat
      541 cgagcaggcc ttgcgtactg tggcggagcg gcatgatctc aaggggagcc tgctcattca
      601 cccgctccgc ctggcggtca ccggcctcag cgtcggaccg gggttgttcg agctgatgga
      661 actcctcggc cgggagacgg tgctgcgccg cctgaccctg gccatcaagc aagtacctat
      721 gatattaggg atcggataag atggaaaacg tcaacggaca ggcggaaaga acggagcagg
      781 gctccggaga ggcggcggtc tcgaccgcgc cccccaattt cattcgcact atcatccgcg
      841 aggatctcaa gaccggcaaa tatcagggac gggtgcacac ctgcttcccg ccggaaccca
      901 acggctatct ccacatcggc cacgccaagt caatttgcct caatttcggc cttgcccgcg
      961 aattcggcgg caagaccaat ctgcgttatg acgacaccaa tcccgccaag gaggatgtcg
     1021 agtatgttga atcgattgag gaagatgtcc gctggctggg ctttgactgg gaagggcgca
     1081 agttctatgc ctccgactat ttcgagcagc tctatgagta tgcggtgcag ctcatcaagc
     1141 tggggatggc ttatgtctgc gatctaagtc ccgatgaaat cagggaatac cggggtactc
     1201 taacagaacc cggcaagaac agcccctatc gcgaccggag cgtagcggaa aatctggatc
     1261 ttttcgagcg catgcgcaag ggtgagttcg cggagggcag ccgtgtgctg cgggcgagga
     1321 tcgacatggc ctcacccaac ctcaatatgc gtgatccggc catctaccgg atcaagaagg
     1381 tcgagcacca tcgcaccggc gatacctggt gcatctatcc catgtacgac tatgcccact
     1441 gtatttctga ttcgatagaa gggatcaccc actccatctg caccctcgaa tttgaggatc
     1501 atcggccgct ttacgactgg tttctcgatc agctcggaat ttaccatccc cagcaaatcg
     1561 aatttgcgcg gctgaacctc tcatataccc tactgagtaa acgcaaactg ctccagctgg
     1621 tgaccgagaa acatgtcagc ggctgggatg acccgcgtat gccgacgatc acgggtattc
     1681 gccgccgcgg ctacaccccg gaatcgatcc gcactttcgc cgaaatcatc ggcgtggcca
     1741 aacgtgacag cgtcgtcgac atccagctgc tggagcactg cctgcgtgag gatctgaatc
     1801 agcgcgcgcc ccggcgtatg gcggtcctca atcccctcag ggtggtgatc gacaactatc
     1861 cggagggaga gttcgaggag ctggatgccg tcaataatcc cgaggatccc gcgatgggca
     1921 cacgcaaggt gcctttcggc cgggaactct acatcgagcg cgatgacttt cgtgaagacc
     1981 cgccgaaaca gttccaccgc ctctcgccgg gacgggaggt caggctgcgt tatgcctact
     2041 ttgtcacctg caccgggctg gaaaagaacg accagggaga agtgacaacc ttgcactgca
     2101 gctacgatcc tgcctcgcgg ggcggcggca cgccggatgg acgcaaggtg aagggcacca
     2161 tccactgggt ctcggctgcg catgccttca acgcgaccgt gcgtctttat gacagccttt
     2221 tcaccaggcc agaccctcag gccacgacgg aaggggaaag ttttctcgac tatctcaatc
     2281 ccgactccct gcgggtgctg accggctgca aactagagcc ctccctggca gaagcggagc
     2341 cgggagcccg gctgcagttc gaacgcaacg gctatttcgt cgcggatagt gtcgattcga
     2401 aaccaggggt tccggtattc aaccgcacag tagccctgcg tgacagctgg gcgaaaatcg
     2461 aaaagaaaat cggcggctga tcaggcgcgg cccacggtca aacacagcac cgcgggcgtt
     2521 ttcagcgcat tcccggcttt cttcccggct gaaaaatttg cttgaattgt cgtaaaaaat
     2581 gcgtatattt actgccatca agccatatcg tccctgggga atcgtccaat ggcaggacac
     2641 atgactctgg atcatggtat cggggttcga atccctgttc cccagctcac aacggaaggt
     2701 ctcagctgaa ccttccgttt ttttttcccc cactcttccc cgctctcccc taccggctct
     2761 ggcacactta ttgtatatat acaagaatga ttcgagctct tttcgcgtcc cgtaaataaa
     2821 ttcaagtgtt tacaacaggt ttgctgccca ctgcggatgg gtggcagccc ctccgattgc
     2881 gaaattgttg ctccgatgtc ccattttgta acagcaggag atgatgtacc tcgccaagtg
     2941 gacacatatt gcgctcacgc tctgcgcggc gttgatgatc cttgtggtgc acctcaagcg
     3001 caccgggacg gagacgccat ctggcgggat agccatggtt ccgctggccg agatcacggc
     3061 cgggccggcg ccggttttct cgatcaatga tccttcgcct cagcctgtga tcaccgcggc
     3121 cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccggctg gtgtattaac
     3241 cccgcctgag cctgcgccgg cattggtatc ggcaggaaga gctggaagta aagttccgga
     3301 gcccgacgtg atgacctcgc agcccgcgga tgcgcctgtg cttccccagc cagtcctggc
     3361 gtcagacgaa aatacgcgac cccttgagag ggcggtagat catcgccttg agagtgcggt
     3421 ggctgctgca gtcaagaaaa cggcgccaca catggagagc cgctccgctt ccggtgagga
     3481 ggtccatcgc caggtcgtcg ctctgatcaa gagatatatt gaggagcagc atggacggga
     3541 ggcccgtccg gtggatttgg ccagtgcctc cagaatggct ccgctgggga tgtcggcgcg
     3601 ggtggtcacc tccccgggca cagggcgctg ggtgctcggt gcatcggccg gccgccaatc
     3661 cctggccggc tccgttcttt gttcggagct ctattggggg cggcgactct cttcagggct
     3721 gcaaatcgga tttaatctct ccaccagtct cgtgaagggg gtgagcgggg aatccggggg
     3781 aggcacaacg ctgctgcagg gcgacctgca gatgcgtctc tttctgcagc cccgggcgaa
     3841 gcttgcaccc tttgtcctac tgggggcagg atatggagag tatcgtgagg aggagtcttc
     3901 gtccgccgcc ccggtcgcca tcgccggagc gggcctggaa taccgtttca gccggcgcct
     3961 cggcgcacag gtgacggcct cctatgaaaa gatgcaggtg aagaagcaac tgccggagag
     4021 cgggggcagc ggccgcgggg tttgggaact gaaggcgggt cttgtgctgg cgatcggcgg
     4081 tccgcgcatg atcgccggtc cggtttcgag cggactggcg gcggcagaat tttctgccga
     4141 acaggcggaa catttaccca gataatacgt ttattattca ggcggtacgt ccttatccct
     4201 ccatcgaatg ccgacccccc taaggcattc aaaaataagg acgtccgcct ttttttattc
     4261 tccggatcga gcaggtgtta tcggcgggcg ggctgcgaga aaaagcttga aaggatatag
     4321 taaaaagtgc tactttatgt gtaagcgatc gggcgctcgt agctcaactg gatagagctt
     4381 ctgacttcgg atcagaaggt tgggggttcg agtcccttcg ggcgcgcaaa aaaaaaagag
     4441 ctcaagtcat ttgagctctt tttttttatc tggcgctcag atacgccggt tggcgatgag
     4501 cagggttttg gggtcgaggc ggagctgcag ccggtcgagc tgccgctcgc cgttgtcctt
     4561 gacgagcttt aattcgatat agtcgcgatg ggccaccagc aggatgacga ccgtcagcgt
     4621 ctctgcgaag gcattgatcg gtgccgggat gatggtttgg tccgtgatcg gcagctcggg
     4681 cggcagggtg gcggagaacc atatttccgc atcgtgctcc tgggcgagct ttttccagaa
     4741 gtggagatcg ctttcgctgg ccttgtcgaa cgaaaagccg tcgacgatga tgatctgggg
     4801 cacaaaggcc gaatccttga tcaggcggac gatgctgctc ttgaccgagt cgaggttgag
     4861 cccgctgcgc ttgaaatgga ggatgaggcg gttgcggatg atctcttcgt agatatccat
     4921 cgcgccggcc agtttgtagg cgcgggcgat ctcatgaaaa acctgggtat accaatgttc
     4981 gatatgttgg ggatcatccg agaaggagat atggagggct ttctggccgc gcagcaggtt
     5041 gtcggtagcg aagtggacaa ggcaggctgt ttttcccacc cccttgcgcg cgacgaagac
     5101 gccgagattg cctttgccca gtccgccgtg aatcgatttt tccaggatcc ggatcggact
     5161 gcgcttgatg atttcttctt tgagcatgag aggcctctga agtaaggtgc gctgctgcag
     5221 gtggcggctc tagtgtaata tacgacagag cgggccatta atgcaacagc ggagtgcagt
     5281 gttttcgtct gaatggccgg ccttcagccg atcagtcgtc tatctgtttt taagcttttc
     5341 ctgatatgcc ttttttagtt cctcggcgat ggactcgggc accttggcgt agcgcttgaa
     5401 ttccatggtg aattctgcct tgccctgtgt ggccgagcgc agaaccgtgg agtagccgaa
     5461 catctcaccg aggggcactt cggcctcgac agcggtgaag gttttgtcct ctgtggcgct
     5521 gaggatcatg ccgcggcgct gattgatggt ggcgagcacc gcgccctgaa actcgggcgg
     5581 ggtctcgacg gcgacgcgca tgaccggctc gagaataatc ggcttgcagc ggctgtaaaa
     5641 ttcccggaag gcgctggagg ccgccgtctg gaaagccatg tcagacgagt cgacaggatg
     5701 gtagttgccg tcattgacga tgaccctgac cccaacgatg ggaaagccga ccagcgggcc
     5761 cttctcgagc gccgcctcaa agcccttttt gcatgacggg acatattcca cggggatgac
     5821 gccgcccttg atctcgtcga cgaattcaaa gacgccctca tgaaagggct cgacctgtcc
     5881 gcagatgcgg gcgtactggc cggcaccgcc ggtctgtttt ttgtgggtat aatcgaaact
     5941 ggccgcctgg gagatgctct cgcggtaggc cacttgtggt gcgccggtct cgagggccac
     6001 cccgtattcg cgccgcatcc gctcgacata gacgtcgagg tgaagctcgc ccataccgcg
     6061 gatgatcgat tcgccggtct cgggatccac gaaggtcttg aaggtcggat cctctttgct
     6121 gaagcggttg agggccttgg ccatgttctc acccgatttg ttgtcaaggg gacggatgga
     6181 gagtgagatc accgggctgg ggacgaacat cgaggtcatg gagtagttga tgtcagtcga
     6241 gcagaaggtg tcgccggagg cgcagtcgat gccgaagagg gcgacgatat cgccggcgct
     6301 cgcctcattg atgtcctcca tctcgtcggc gtgcatgcgg ataagtcggc caatgcgcac
     6361 ctttttctgg gaacggctgt tgcgcagctc atcgcccttg cggatcatcc cctggtagat
     6421 gcgcacgtag gtaagctggc cgaagcgggt gtcgtcgagc ttgaaggcga gcagcacggc
     6481 tggcagggcg ttgtcgggtt ggaggagaat ttcggtttcg ccggcgtcca ggtccagggc
     6541 acggttttcg atttcggtgg gcgcgggcag atagtcgacc actccgtcca gcagggtctg
     6601 gatgcccttg tttttgaaag cggagccgat gaagaccggg gtgagttcaa gggccagggt
     6661 gccctggcgc acggcctgat agatgagttc ttcggtgacc cggtcttcga ggtaggcctc
     6721 ggcgagtgcg tcactgaaga gagaggcacg atcgatgagg agatcgcgtt tttccagggc
     6781 cttttcgagc agcccggccg ggacctcttc ttcgcgcagg atctcgccgt tactgccgtc
     6841 gaagtagatg gctttcatgc ggataaggtc gataatcgcc tcgaaattgg cttcgaggcc
     6901 gatcgggagc tgcatcagta ctgcgttatg gccgagtttg tcgcgcagct gttcgacgat
     6961 gcgttccgga tcggcgccca tgcgatcgca cttgttgatg aaggcgaggc gggggacctt
     7021 gtatcg
//
LOCUS       DAOPWU010000172         7022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10702_length_7022_cov_4.351514, whole genome shotgun sequence.
ACCESSION   DAOPWU010000172 DAOPWU010000000
VERSION     DAOPWU010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10702_length_7022_cov_4.351514"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /gene="dnaB"
                     /locus_tag="PKM23_09470"
     CDS             complement(<1..1230)
                     /gene="dnaB"
                     /locus_tag="PKM23_09470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNY91741.1"
                     /translation="MNEQTDIPVKLPPQSIEAEMAALGSVMIEPHAANTLFELLDETC
                     FYKDAHRRIFIAARNLYSHSQAIDVLTVSSELEKSKELDKVGGHYYLTELVERIPSAA
                     NIEQYCRIVLNRALLRKLIEVAGEIQQDCYEGGEDAYDVIDVAEKKIFSLSERSKRRS
                     FEHLRPILFETMNLIDHYNAKADGVIGVPTGFKDLDRLLSGLQNSELVILAARPSMGK
                     TALALNMARTAAGYGVPVGIFSLEMASPQIAMRLLCSEARVDAHLVRIGKLPPDQMAK
                     LMNALGTLEKAPIYIDDSPAMTIMEIRSKARRLKIEYNIGLLVVDYLQLVHGVGRVES
                     RQIEIAQISQSLKALAKELDIPVLALSQLSRAVEARGERKPMLSDLRESGAIEQDADV
                     VMFIYRPSVYGPVEPGQE"
     gene            complement(1242..2030)
                     /locus_tag="PKM23_09475"
     CDS             complement(1242..2030)
                     /locus_tag="PKM23_09475"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNY91742.1"
                     /translation="MSGDAESDLRRFLEQQIELYGDELALPKEKLNWRGGEAATPAPG
                     RSPYLGERIREGLESPPRPEPQAMPDLNLSTLEELQGRISGCLRCAIGGRRRNLVFGE
                     GNPAADIVLVGEAPGFHEDQQGLPFVGEAGQLLTRILASIQLQRSEVYICNLLKCRPD
                     NNRDPLPDEVANCLPYLEKQLALIQPAFILCLGRFSAQTLLQSQASLTQLRGKVHPWR
                     GSRLIVTYHPAALLRFPKFKHETWQDVQLLRRLYDEFLSENRPR"
     gene            complement(2027..3232)
                     /gene="coaBC"
                     /locus_tag="PKM23_09480"
     CDS             complement(2027..3232)
                     /gene="coaBC"
                     /locus_tag="PKM23_09480"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNY91743.1"
                     /translation="MLTNRKVVVGVTGGIAAYKAAELVRQLARLGASIRVIMTPAAME
                     FITPLTFETLTGHPVLTGLFPHEQGGGTVHIEWARWAECLVICPATANTIARAAHGFA
                     DNALTTTLLATTAPILFCPAMNRQMYANPLYQQNEKRLFEHGWSVVAPGSGELACGEV
                     GEGRLAEIPEIVEAVRRLLSRKHDYQGRRVLVTAGPTHEPLDPVRYLGNRSTGKMGYA
                     LAEMAALRGAGVTLVTGPSHERPFSGIKVVRVTTAAEMSEAVQHHLPDCDLLFMAAAV
                     SDYRPAAVAAQKIKKGEEEIILPLTRTEDILRSAGLSKGGRTHVGFSVETSEEIPASL
                     RKLEEKNLDMIVVNNPLEPGAGFAVDTNIITLLRRGRDPEKWPQMSKQEVADRLLTAV
                     LEWEKEKSL"
     gene            complement(3257..3532)
                     /locus_tag="PKM23_09485"
     CDS             complement(3257..3532)
                     /locus_tag="PKM23_09485"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNY91744.1"
                     /translation="MIKTLPIDNLDQFADNVYEAIIMIARRARQINDLQKKVLDAEAA
                     SISNNDNYDDEGVSQDVVDRQYLKLPKPTSVALQEMLEGKLTKQYPG"
     gene            complement(3573..4160)
                     /gene="gmk"
                     /locus_tag="PKM23_09490"
     CDS             complement(3573..4160)
                     /gene="gmk"
                     /locus_tag="PKM23_09490"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNY91745.1"
                     /translation="MTHKGLLVVISSPSGGGKSTIIRRIISEGDPRCRYSISATTRAM
                     RPGEKDGIDYWFVDEAAFTEMIRRDELAEYEQVHGHLYGTPVRPIEKWLQEGCIVFFD
                     IDVKGAFAIQRRFPDETLMIFIAPPDLETLRQRLVGRGTEASEAVRIRLERVPMEMEM
                     GKAFEFTVINRDLHRSIARVKKIIAGRLAERGAVA"
     gene            complement(4176..5054)
                     /locus_tag="PKM23_09495"
     CDS             complement(4176..5054)
                     /locus_tag="PKM23_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNY91746.1"
                     /translation="MISSMTGFGRASHKIGDIEVLVEIRSVNNRFLDFSLRIPRVLAD
                     YESQIKELVGKSLSRGRINIAISLTSESSNVQQLVLNRPLAQSYSRLVKELQKDLGLA
                     GEIQLSQLLNLPDIIVVDDKTPEAERYWECTRAALVKVLEDIQVMRHKEGEELLKDFV
                     RRINSLDGMITRAAELGARQPAAEMEKLRQRIALLVKDEHIDEGRLENELALMADRLD
                     ITEECVRFHSHNKAFIHILENESAPGRKLNFLLQEMHREVNTIGSKSNSLEISHLVVE
                     IKDEVEKLREQIQNIE"
     gene            complement(5183..6205)
                     /locus_tag="PKM23_09500"
     CDS             complement(5183..6205)
                     /locus_tag="PKM23_09500"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY91747.1"
                     /translation="MIKRIFPSLTGILLLLITFARGAEIRKYAGEFMAGGVGARALGM
                     GGAYVAVTGDVTFGYWNPAGLSSIRYPELGFMHARRFGGVVNYDYFGGALPVTRRETI
                     GVNVVRLAVDDIPITALPRPDLPLDATYTDGKGNTLSNRPYQVRTVNDAEYAVYLSYA
                     RMQTGRFAWGASAKLVHKGVGDNAAWGAGFDLGAIWLPWRSLRLGANLQDFTTTLLAW
                     DTGERELVSPTLKLGGAYPFIFRPLKSRLLLAADADLRMEGRKLAAQAHYGPMSADFH
                     LGAEWVFHNIASVRIGSDTGCLSAGAGLRLPRIDVDYAFLKHDQLDNSHRISLRVRIE
                     ERKARK"
     gene            complement(6356..>7022)
                     /locus_tag="PKM23_09505"
     CDS             complement(6356..>7022)
                     /locus_tag="PKM23_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNY91748.1"
                     /translation="EIGLVDRVFTRVGASDNLAAGESTFLTEMVETANILNNATPRSL
                     ILLDEIGRGTSTFDGLSIAWSVAEFIHETPRIAAKTIFATHYHELTELELVLTRVRNY
                     NVAVKEWGDRIVFLRKIVPGGCDHSYGIHVAQLAGLPRAVIMRAREILANLESGSLDP
                     DKGPRLAHHQSEMKRPVQQLSLFAELEQSLREELKKLDPNQLTPLEALNKLEELRQLA
                     SDR"
BASE COUNT         1294 a         2030 c         2266 g         1432 t
ORIGIN      
        1 ctcctggccg ggctcgaccg ggccgtagac cgaggggcgg tagatgaaca tgaccacatc
       61 ggcatcctgc tcgatcgccc ccgactcgcg caaatcggag agcatcggct tgcgctcgcc
      121 gcgggcctcg acggcgcgcg agagctggga gagggccagg accgggatat cgagctcctt
      181 ggcgagggcc ttgagggact gcgaaatctg ggcgatctca atctggcggc tctcgacccg
      241 gccgacgccg tgcacgagct gaagatagtc gacgacgagc aggccgatat tgtactcgat
      301 cttgaggcgg cgcgccttgg agcggatctc catgatggtc atggcgggag aatcgtcaat
      361 atagatgggg gccttttcga gggtgcccag ggcgttcatc agtttggcca tctggtccgg
      421 aggcagcttg ccgatgcgca ccagatgggc gtcaacccgg gcctcggagc agagcagacg
      481 catggcaatc tggggtgagg ccatttccag ggagaagatg ccgaccggca ccccgtagcc
      541 ggcggcagtg cgggccatgt tcagggccag agcggtcttg cccatcgaag gcctggcggc
      601 gaggatgacc agttcggagt tctgcaggcc ggagagcagg cggtcgaggt ccttgaaacc
      661 ggtcggcaca ccgatgaccc cgtcggcctt ggcattgtaa tggtcgataa ggttcatggt
      721 ctcgaagagg atcggccgca gatgctcgaa agacctgcgc ttgctgcgct cggagaggct
      781 gaagatcttt ttctccgcca catctatgac atcataggca tcttccccgc cctcgtagca
      841 gtcctgttgt atctcaccgg ccacctcaat gagcttgcgc agcagcgcgc ggttgaggac
      901 aatgcggcag tactgctcga tattggctgc cgaagggatg cgctccacca gttcggtgag
      961 gtaatagtgc ccgcccacct tgtcgagctc cttgctcttc tccagctcgg aggagacggt
     1021 caggacgtcg atggcctggc tgtgggaata gaggttgcgc gcggcgataa agatgcgacg
     1081 atgagcatcc ttgtaaaagc aggtttcatc gaggagttcg aagagggtgt tggcggcatg
     1141 gggctcgatc atcaccgaac cgagggccgc catttccgct tcgatcgact gcggcggcag
     1201 cttgacgggg atatcagtct gttcattcat ggtcagctcg ctcagcgtgg acggttctcg
     1261 gacagaaatt catcatagag acggcggagc agctggacat cctgccaggt ctcgtgcttg
     1321 aatttgggaa agcggagcag ggctgccgga tgataggtga cgatgaggcg ggagcccctc
     1381 caggggtgga ccttgccgcg cagctgcgta agggacgcct ggctctgcag cagggtctgg
     1441 gcgctgaagc gccccaaaca aagaatgaag gccggctgga tcagcgccag ctgtttctcg
     1501 aggtagggca ggcagttggc cacctcgtcc ggcaaaggat cgcggttgtt gtccggccgg
     1561 catttgagca gattgcagat atagacttcg ctgcgctgga gctggatgga ggccagaatc
     1621 ctggtgagaa gctgcccggc ttcgcctacg aagggaagcc cctgctggtc ttcatgaaaa
     1681 ccgggggcct cgccgaccag aacgatgtcg gcggcgggat tgccctcgcc gaaaaccaga
     1741 tttctgcgcc ggccgccgat ggcgcagcgc aggcagccgc tgatcctgcc ctgcagctcc
     1801 tcgagggtgg agagattgag gtccggcatg gcctggggtt cgggccgggg cgggctctcc
     1861 agcccctcgc ggatgcgctc gccaagatag ggcgaacggc cgggagcagg cgtggccgcc
     1921 tcccctcctc gccagttcag cttctcttta ggcagggcaa gctcgtcgcc gtagagctca
     1981 atctgctgtt cgagaaaccg gcgcagatcc gattcagcat cgccactcat aggctcttct
     2041 ctttttccca ctccaggacg gccgtcagca gccggtcggc cacctcctgc ttggacattt
     2101 gcggccactt ttcggggtca cgcccgcgcc gcagcagggt gatgatattg gtatccacgg
     2161 cgaaaccggc gcccggttcg aggggattgt tgacaacgat catgtcaaga ttcttctcct
     2221 cgagtttgcg cagggaggcg ggaatctcct cgctggtctc aacggaaaag ccgacatggg
     2281 tgcggccgcc cttgctcagt ccggcgctcc tgaggatgtc ttcggtccgg gtcaggggca
     2341 gaatgatctc ctcctcccct ttcttgatct tttgcgccgc aacagcggcg ggccggtaat
     2401 cgctgacagc ggcggccatg aaaagaagat cgcagtcggg caggtggtgc tggaccgctt
     2461 cagacatctc ggcggcggtg gtgacgcgga caaccttgat gccggagaag gggcgctcat
     2521 ggctcgggcc ggtcaccagg gtcaccccgg ccccgcgcag ggcggccatt tcggccaggg
     2581 cgtagcccat cttgccggtg gagcggttgc cgagatagcg cacgggatca aggggttcat
     2641 gggtcgggcc tgctgtcacc agcacgcgcc tgccctggta atcatgcttc cggctgagga
     2701 gcctgcgcac ggcctcgacg atctccggga tttcagccag gcggccctct cccacctcac
     2761 cgcaggcgag ctcgccgctc ccgggcgcga caacgctcca gccgtgctcg aacagacgct
     2821 tttcattctg ttgataaagg ggattggcat acatctgcct gttcatggcc ggacagaaga
     2881 ggatgggagc ggtggtggca agcagggtgg tggtaagggc attgtccgca aaaccgtggg
     2941 cagccctggc gatggtattg gccgtcgccg ggcagatgac caagcattcc gcccagcgcg
     3001 cccattcgat gtgtacggtg cctcccccct gctcatgggg gaagagcccg gtgagaactg
     3061 ggtgaccggt caaggtttcg aaggtcagcg gtgtgatgaa ctccatggcc gcgggggtca
     3121 tgatcacgcg gatggaggcc ccgagtcggg cgagctgtcg taccaattcc gcagccttgt
     3181 aggccgcgat accgccagtc acgccaacca ctactttcct gttcgtcagc atgcggcact
     3241 ctgctcctga gtggtactag ccggggtact gcttggtgag tttgccctca agcatctcct
     3301 gcaaggcaac gctggtgggt ttgggcagtt tgaggtactg gcggtcgacg acatcctggc
     3361 tgaccccttc gtcgtcataa ttgtcgttgt tgcttatcga ggcagcttcg gcgtcgagca
     3421 cctttttctg caggtcgttg atctggcggg cgcggcgggc gatcatgatg atcgcttcat
     3481 agacattatc ggcaaactga tcaaggttat caatgggcag tgttttgatc atatagatgg
     3541 cctcccggta tgtatgattt gatttccatg ttttatgcaa ctgcgccgcg ctcagcgagc
     3601 cgaccggcga tgattttttt aactctggcg atgctcctgt gcagatcacg gttgattacc
     3661 gtgaactcaa aagccttgcc catctccatc tccatcggca cccgctcgag gcggattcgc
     3721 accgcctccg aggcctcggt gccgcgcccg accagccgct gtcgcagggt ctccagatcg
     3781 gggggtgcga tgaagatcat caaagtctca tcagggaacc ggcgctggat ggcaaaggcc
     3841 cccttgacat cgatatcgaa aaagacgatg cacccctcct gcagccactt ttcaatcggc
     3901 cggaccgggg ttccgtagag atggccatgc acctgctcat attcagccag ctcatccctg
     3961 cgaatcatct ctgtgaaagc ggcctcatcg acgaaccagt aatcgatccc gtccttctcg
     4021 ccagggcgca tggccctggt cgttgctgaa atggaatagc gacagcgggg atccccctcg
     4081 ctgatgatcc tgcggatgat cgtcgacttg ccgccgccgg agggcgaaga gatcaccacg
     4141 agaaggccct tgtgcgtcat gcggagcccc tcggcttatt cgatgttctg gatctgttcg
     4201 cgcagctttt caacctcgtc cttgatctcg acaacgagat gggagatctc gagggaattt
     4261 gacttgctgc cgatcgtgtt gacctcgcga tgcatctcct gcagtaaaaa gttcagcttc
     4321 cggccggggg cgctttcgtt ctcgagaata tggataaagg ccttgttgtg gctgtgaaaa
     4381 cggacgcact cttcggtgat atcgaggcgg tccgccatga gggcgagctc gttctcgagc
     4441 cgcccttcat caatatgttc gtccttgacc agcagggcga tgcgctggcg cagcttttcc
     4501 atctcggcgg cgggctgtcg ggcgccgagc tcggctgccc gcgtgatcat accgtcgaga
     4561 ctgttgatgc gccggacgaa atccttgagc agctcttcgc cctccttgtg gcgcatcacc
     4621 tggatatcct ccagcacctt gaccagggcc gcccgcgtgc actcccagta gcgctccgcc
     4681 tcaggggttt tgtcatcgac gacgatgata tcaggcaggt tcagcagctg gctgagctgg
     4741 atctccccgg cgaggccgag gtctttctgc agctccttga ccaggcgaga gtaggattgg
     4801 gccaggggcc ggttgaggac cagctgctgg acattggagc tctcgctggt cagactgatg
     4861 gcgatgttga tgcggccgcg agaaaggctc ttgccgacca gttccttgat ctggctttcg
     4921 taatcagcca aaacccgcgg gatgcgcagg gagaaatcga gaaagcggtt gttgaccgaa
     4981 cggatctcca caagcacttc gatgtcaccg atcttgtggg aggcgcggcc gaaaccggtc
     5041 atgcttgaaa tcatggtaat atcctgatta tggcagctct gattaaatca gcatttattt
     5101 taatgatttt tttcgatata gtcaatcctt ttttcaggat tgcgcagccg gcgggcaggc
     5161 tgctgccggc aggggcggag ggctatttac gggccttgcg ttcttcgatc cgcacccgca
     5221 gcgagatgcg gtgcgaattg tccagctgat catgcttgag aaaggcataa tcgacatcga
     5281 tccggggcaa gcgcagtccg gcgccggcgc tgaggcagcc ggtgtcgctg ccgatgcgca
     5341 ccgaggcgat gttgtgaaaa acccattcgg cgccgagatg aaaatctgca ctcatgggcc
     5401 cataatgggc ctgggcggcg agcttgcgcc cttccatgcg tagatcggcg tccgccgcca
     5461 gcagcagacg gcttttgagg ggtctgaaga tgaagggata ggctcccccg agcttgaggg
     5521 tgggcgagac cagctcccgc tccccggtat cccaggcaag cagggtggtg gtgaaatcct
     5581 gcagattggc gcccaggcgc agggaacgcc atggcagcca gattgcgccg agatcaaaac
     5641 cggctcccca ggcggcgttg tcgccaaccc ccttgtgcac cagtttggcg ctcgcgcccc
     5701 aggcgaagcg gccggtttgc atccgggcat agctgagata gacagcatac tcggcgtcgt
     5761 tgaccgtgcg cacctggtaa gggcgattgc tgagggtgtt gcccttgccg tcggtatagg
     5821 tggcatcgag gggcagatcg ggtctcggca gcgctgtgat cgggatgtca tcgacggcga
     5881 ggcggacgac gttgacgccg atagtctccc ggcgggtgac cggcaaggcc ccgccgaaat
     5941 agtcgtaatt gaccacgccg ccgaatcgcc gcgcatgcat gaaccccagt tcggggtaac
     6001 ggatgctgga gaggccggcc ggattccagt agccgaaggt aacatcgccg gtgaccgcga
     6061 cataggcccc gcccatgccg agagcgcggg cgccgacgcc gccagccatg aattcaccgg
     6121 cgtatttcct gatttccgcc ccccgggcga aggtaatgag gagaaggaga atccccgtca
     6181 gcgatggaaa gatgcgtttg atcacgcagg tacctctggt tgcatgcacc aatctgccgt
     6241 tgcggcgacc tttggtcgct gtttctataa tttatgatta aatccggatt gtacaagcga
     6301 ataatcttcc ggcagcggcc cgtaagacat tttgcgcccc gggcttgcag ggcgcctacc
     6361 ggtcggaggc cagctgccgc agctcttcga gcttgttgag cgcctcgaga ggggtgagct
     6421 ggttggggtc aagtttcttg agctcctcgc gcaggctctg ttccagttcg gcgaaaaggc
     6481 tgagctgctg caccggccgt ttcatctcgc tctgatggtg cgccagccgg ggtcccttgt
     6541 ccggatcgag ggaaccggat tcgaggttgg cgagaatctc ccgcgcgcgc ataatgacgg
     6601 cgcgcggcag gccggcgagc tgggcgacgt ggatgccgta gctgtggtcg cagccgcccg
     6661 gcacgatctt gcgcagaaag acgatgcggt caccccactc cttcaccgcc acattgtagt
     6721 ttcttacacg cgtcagcacg agttccagtt ccgtcaattc atgataatgg gtagcgaaga
     6781 tggtttttgc agcaatacgc ggggtttcat gaatgaactc ggccaccgac caggcgatgg
     6841 agaggccgtc gaaggtgctg gtgccgcggc cgatctcatc cagcaagatg aggctgcgcg
     6901 gtgtggcgtt gttgaggatg ttggcggtct cgaccatctc ggtgaggaag gtgctctccc
     6961 ccgccgccag gttgtccgag gcgccaacgc gggtgaagac gcggtcgacc agcccgatct
     7021 cg
//
LOCUS       DAOPWU010000173         6999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10739_length_6999_cov_4.308612, whole genome shotgun sequence.
ACCESSION   DAOPWU010000173 DAOPWU010000000
VERSION     DAOPWU010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10739_length_6999_cov_4.308612"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PKM23_09510"
     CDS             complement(<1..837)
                     /locus_tag="PKM23_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNY91749.1"
                     /translation="MNEKMAEPENSNWSYLAYDHIQGQWRPPANTWRIVGRKYVNLIL
                     FLATILSTWLVGGIWYCLAIIAILTAHEMGHFLMCRKYGVPATLPYFIPFPFPSYNPF
                     GTMGALISMRGYIPSRKALFDIGAGGPLAGLAVTISVLFIGLALSTTAPRAETAGGLA
                     LGESLLFKLISLIVLGPQPDEIDIMLHPVAYAGWAGLFITGLNLIPIGQLDGGHVTYA
                     LFGPRAKYFTWLFIGALGILSIFNYSWALLFLLLAIFGRRHPSPLDDYTRLDHRRRII
                     GAL"
     gene            complement(834..1514)
                     /locus_tag="PKM23_09515"
     CDS             complement(834..1514)
                     /locus_tag="PKM23_09515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNY91750.1"
                     /translation="MAESQHTPDRPNTREPFYLAIAGNIGVGKTTLTQMISSHFGWTA
                     YFERVINNPYLDDFYADMNRWSFNLQVYFLSRRFMDQRLISSNPESCVQDRTIYEDAE
                     IFAYILHNQGNMSDRDYSNYRDLFYTMTEYLRKPHLILYLRASTWTLISRIRKRGRDF
                     EKNITNEYLFELNAAYERWIGEIRKTIPVLVVDADKVDFEKSAADFEAICQTISEHQK
                     AHRRGDRE"
     gene            complement(1518..2900)
                     /locus_tag="PKM23_09520"
     CDS             complement(1518..2900)
                     /locus_tag="PKM23_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY91751.1"
                     /translation="MTRRSSNPRILLVNPWIHDFSAFDFWQKPLGLLYIGAILNRMGC
                     ETLLLDCLDRYNPFWAPLSGSAEAGEKIEGAGKYFRQEVEKPAPLHGIPRRFCRYGAP
                     RERVEAWLEKEAPPDYILMTSFMTYWYPGLVEMAELLRARYPHAPLILGGIYATLCPD
                     HARRTVRPDHLITGEGETRVVSLITELSGVPGADSLGLDIERLPYPWYEGYPHLTSIA
                     MLTSRGCPYHCSYCASGLLAASYRRRSPEAVAREIIHWRDTRGVSTIAFYDDALLHEP
                     ELHAKPLFRRLAGLKPAIALHTPNGVQPRCIDEEMAGLMHAAGVKSLRLSYESSSFLR
                     RQGKVTARELSSALKNLFAAGFQSGQIGVYILAGLPEQEMEEVRDTARQVHVLGARVN
                     LASYSPIPGTREYRRALESGLWDKHQDLLLGNNSLYPFWRRKYAPEELDGLTLWVKEL
                     NQSLLPNHEE"
     gene            complement(2908..4074)
                     /locus_tag="PKM23_09525"
     CDS             complement(2908..4074)
                     /locus_tag="PKM23_09525"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNY91752.1"
                     /translation="MAEIKRPNHTILVVEDDPNLRRLFVEILRRDSHIVLEADSGEAA
                     MSKIEAFELDVVISDMQMQKLSGLDVLKAATRKDPNTQVIIITGFASVGTAVRAMREG
                     AYEYLTKPVQPDALTIKVRNAIERRRLLITLAEQEKTLQMHHEMIDRDLILARQIQAS
                     LVPSNFTTDGIDIGIRYRPMIGLGGDFADFYRIDEERLYLVVIDVTGHGIAAALLVSR
                     VCHEWRRAVQQAPGPGELLWQLNEFFIETFFDTPLFLTIMAVEVNLRQRRLRYAGSAH
                     PAALLQRNGQGFVRLDSQNAIIGFAQADKSTFHSASLSYKPGDNLLIYTDGLVEAETT
                     GGKILGLQGLQRIIRQEARGSTALKTAEAIVDRVVEFTRAEPGDDMLMTVAHLF"
     gene            complement(4062..5792)
                     /locus_tag="PKM23_09530"
     CDS             complement(4062..5792)
                     /locus_tag="PKM23_09530"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNY91753.1"
                     /translation="MARELDERLVELQSLFEMSQVLNSSLQLRSTLNNLLLTPMGRMM
                     ISRGIVLVVNEHQAFEVAALKGLPRTLMGKKVFCTGELPDSFVVADIRETDEKLKAFF
                     EENRIDLVIPIISVQRTVGIMGLGSKLGGIPFTPAETEYLTSLSNIAASSIENALMYQ
                     KLELVNRRLDKKVQELRTLFEIGKELNANLEIDRIVNLLIYAVMGELVVNRCYIYLRR
                     DDALQLFASRGMRPDDPNCQLLNAPGFISALAGVDRVLNVDEEEPAPGLQLLREMHLK
                     LVIPMQMEEEIRGLFVVGEKIKAQPFQPDEIEFLSTLCNLAMISIENARLFAEALEKE
                     RIEEELNIARDIQQRLLPRGCPGNDYIEIRGLNIPTYQVGGDYFDCIRLDDNRLAVAI
                     ADVSGKGVPASLLMSSLHAGLHNLVGIGGDIPAMVGRLNNFIQANTGYDKFITFFYGE
                     FDLVGKSFTYVNAGHNLPYLFRADGSIRRLETGGLLLGMLPDMPYASETIPMQKGDLL
                     LLFTDGVTEAKSASDEDFEEERLEAVACSVLHLPLQELLTALVDAVRIHSTGMPQSDD
                     ITMMAVRWLK"
     gene            complement(5836..6294)
                     /locus_tag="PKM23_09535"
     CDS             complement(5836..6294)
                     /locus_tag="PKM23_09535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91754.1"
                     /translation="MPKAKIEYKLRIPSQTENLEIIRFFVGKVAEKVGFAEDDVGKIE
                     LAVDEACTNVMKHAYTETVKDKTLDVAILIDYQKLTLVVTDHGKGFNPDKIRQPDMKE
                     YLAELRVGGLGIFLMRTLMDEVDYDIKPGVRNQVRMVKYLINRKNSKIVN"
     gene            complement(6378..6749)
                     /locus_tag="PKM23_09540"
     CDS             complement(6378..6749)
                     /locus_tag="PKM23_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY91755.1"
                     /translation="MENFKVVRLDGVNLTTLKIIGFLDAHTAPTLEEAIQKLLDEEQV
                     NILVDFKELSYISSAGLGVFMGFIEEVRSKNGDIKMCAMSPKIYRVFDLLGFPTIYQI
                     FDKQEEALATFPQNSAASSSE"
     gene            complement(6787..>6999)
                     /locus_tag="PKM23_09545"
     CDS             complement(6787..>6999)
                     /locus_tag="PKM23_09545"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNY91756.1"
                     /translation="DTFLFYTDGAVEARNARGEEFTETRLVSLFQRHHALPPEQLQAR
                     LIEEISGFVGSARSHDDLTFVILQAV"
BASE COUNT         1491 a         1841 c         2179 g         1488 t
ORIGIN      
        1 cagggcgccg atgatgcggc ggcggtgatc caggcgggta taatcatcga ggggcgaggg
       61 atgccgcctg ccgaagatgg ccagcagcag aaaaagcagc gcccagctgt agttgaaaat
      121 ggagaggatg ccgagagcgc cgatgaagag ccaggtgaag tatttggcgc gtgggccgaa
      181 aagggcatag gtgacatggc cgccgtcgag ctgtccgatg gggatcaggt tgaggccggt
      241 gataaacaga ccagcccagc cggcgtaggc caccggatga agcatgatgt cgatctcgtc
      301 gggctggggc ccgagaacga taagggagat cagcttgaag agcagggatt ctcccagggc
      361 gagcccccca gcagtctcgg cacggggggc ggtagtggag agggcgagcc cgatgaagag
      421 gacggatatg gtcacggcca gtccggcaag gggtccgcca gcgccaatgt cgaagagggc
      481 tttacggctc ggtatgtagc ctcgcatgga gatgagggcc cccatggtgc caaaggggtt
      541 atagctcgga aaagggaagg ggataaaata gggcagtgtg gccggtacgc cgtatttgcg
      601 gcacatgaga aaatggccca tttcgtgtgc ggtcagaatg gcgatgatgg caaggcagta
      661 ccagatgccg ccaacgagcc aggtggacaa gatagtagca aggaagagaa tgagattgac
      721 atactttctt ccgacgatgc gccaggtatt agcgggtgga cgccactgac cctggatatg
      781 gtcataggcc aggtaggacc agttgctgtt ttcgggttca gccatttttt cgttcattcc
      841 ctgtcaccgc ggcggtgcgc cttttggtgt tcgctgatcg tctggcagat cgcctcaaaa
      901 tcagcagcag atttctcaaa gtctaccttg tcggcatcga cgaccaacac cggaatggtt
      961 ttccggattt ctcctatcca acgctcgtag gcggcattaa gttcgaagag atattcattg
     1021 gtgatatttt tttcgaaatc ccttcctcgt ttgcgaatac gggaaatgag ggtccaggtg
     1081 gaggcgcgca gatagaggat gaggtgcggc ttgcgcaaat actcggtcat ggtatagaag
     1141 aggtcgcggt aattgctata gtcccgatcg gacatgttgc cctggttgtg cagaatatag
     1201 gcgaagatct cggcgtcctc gtagatggtg cggtcctgga cgcaggattc cggattggat
     1261 gagatgaggc gctggtccat gaagcggcgg gagaggaaat agacctgcag gttgaaactc
     1321 cagcgattca tgtccgcata aaagtcatcg aggtagggat tgttgatcac ccgttcaaag
     1381 taggcggtcc agccgaaatg gctgctgatc atctgcgtca gggtggtctt gccgacgccg
     1441 atattgccgg cgatggcaag ataaaaaggc tccctggtat tcggccggtc cggggtgtgc
     1501 tgcgattccg ccatatttca ttcctcgtga ttgggaagca gtgattggtt gagttctttc
     1561 acccagaggg tcaatccgtc cagctcctcc ggagcgtatt tccgtctcca gaacgggtaa
     1621 agcgaattgt tgccgaggag gaggtcctga tgtttgtccc agaggccgct ctccagggcg
     1681 cgtcgatatt cccgggtgcc ggggatcgga gagtaggagg ccaggttgac acgggcgccc
     1741 agaacatgaa cctgacgggc ggtgtcgcga acctcttcca tttcctgctc gggcagtcct
     1801 gcaaggatat aaacaccgat ctgtccgctc tgaaatcccg ctgcgaagag gtttttcagg
     1861 gctgaagaga gttcccgggc agtgaccttg ccctggcggc ggaggaagct gctgctctca
     1921 tagctgagac gcaggctctt cacgccggcc gcatgcatca agccggccat ctcctcatcg
     1981 atgcagcggg gctgcacacc gttcggggta tgaagggcaa tggccggttt gagcccggcg
     2041 agcctccgga aaaggggctt ggcatgcagc tccggttcat gcagaagggc atcatcatag
     2101 aatgcgatgg tactgactcc gcgggtatcc cgccaatgga tgatctccct ggcaaccgct
     2161 tccgggctgc ggcggcggta ggaggctgcg agcagtccgg aggcgcagta gctgcagtga
     2221 taggggcagc cgcgtgaggt gagcatggcg atgctcgtca ggtgcgggta gccctcgtac
     2281 cagggatagg gcagccgctc gatatccagc cctaaagaat cagcccccgg aaccccggag
     2341 agttcggtga tcaggctcac cacccgggtt tcaccctccc cggtaatgag gtgatccggt
     2401 ctgactgtcc ggcgggcgtg atcggggcaa agcgtggcgt agatcccgcc caggatcaag
     2461 ggggcgtgtg gataacgggc gcgcagcagc tccgccatct cgaccaggcc aggataccag
     2521 taggtcatga aggaggtcat gagaatataa tcgggtgggg cctccttctc cagccacgcc
     2581 tccacccttt cgcgcggggc gccatagcgg caaaagcgcc gtggtatccc atgaaggggc
     2641 gccggtttct ccacctcctg gcggaaatac ttgccagccc cctcgatctt ctccccggcc
     2701 tccgcgctgc cggatagcgg agcccagaaa ggattgtaac ggtccaggca gtcgaggagg
     2761 agagtttcgc atcccatgcg gttgaggatg gcgccgatat aaagcaagcc caggggcttt
     2821 tgccaaaaat cgaaggccga gaaatcatgg atccagggat tgaccagcag gatccgggga
     2881 tttgagctgc gcctcgtcat ggagcgctca gaagagatgg gcgaccgtca tgagcatatc
     2941 atcgccaggt tcggcgcggg tgaactcaac cacgcgatcg acaatcgctt cggcagtttt
     3001 gagcgcagtg gagcccctcg cctcctggcg gatgatgcgc tgcagcccct ggagaccgag
     3061 tattttcccg ccggtggtct ccgcctcgac caggccatcg gtgtagatga gcaggttgtc
     3121 gcccggtttg taggaaagtg aggctgagtg aaacgtcgac ttgtcggctt gcgcaaagcc
     3181 gatgatggca ttctgcgaat caagccgcac gaatccctgg ccgttgcgct gcagaagagc
     3241 ggcgggatgg gcgctgccgg cataacgcag ccgtcgctgg cgaagattga cctccacggc
     3301 catgatggtc agaaagagcg gggtatcaaa aaaagtttcg atgaagaatt cgttcaattg
     3361 ccaaagcagc tccccgggac cgggagcctg ctgtacggcc cttctccatt catggcagac
     3421 ccggctgacc aatagggcgg cggcgatgcc gtggccagtg acgtcgatga ccacgaggta
     3481 gagtctctcc tcatcgatgc gatagaaatc ggcaaaatcg ccgcccaggc cgatcatggg
     3541 gcggtagcga atgccgatgt cgatcccgtc cgtggtgaag ttggagggga cgagactggc
     3601 ctgaatctgg cgggcgagga tgagatcgcg gtcgatcatc tcgtgatgca tctggagggt
     3661 tttttcctgt tccgccaggg taatcaggag acggcgcctt tcgatggcgt tgcggacctt
     3721 gatggtgagg gcatcgggct ggaccggttt ggtcaggtac tcataggcgc cttcgcgcat
     3781 ggcgcgcacg gcagtgccga cggaggcgaa tccggtaatg atgatcacct gggtattggg
     3841 gtccttcctg gtcgccgcct tgagcacatc gagtccgctg agtttttgca tctgcatatc
     3901 gctgatgacc acatccagtt caaaagcctc gatcttgctc atggcggcct caccggaatc
     3961 ggcctccagg acaatatggg agtcccgcct caggatttcg acgaagagcc tgcgcaggtt
     4021 gggatcatcc tcgacgacga ggatagtgtg gttgggccgt tttatttcag ccatcgtacc
     4081 gccatcatgg tgatatcatc ggattggggc atacctgtgc tgtgtatgcg gacggcatcg
     4141 accagggcag tgagaagctc ctgcagaggg aggtgtaaaa ccgagcaggc aaccgcctcc
     4201 agcctctcct cctcaaaatc ttcatcgctg gcgcttttgg cttcagtcac cccgtcggtg
     4261 aaaaggagca gcagatcccc cttttgcatg gggatggttt cggaggcata gggcatatca
     4321 ggcagcatgc ccaatagcag ccccccggtc tccagccgcc gaatggagcc gtcggcgcga
     4381 aagagatagg gcagattgtg cccggcgttg acataggtga agctcttgcc gacgagatcg
     4441 aattcaccat agaaaaaggt gatgaatttg tcgtagccgg tattggcctg gatgaaatta
     4501 ttcagccttc ccaccatggc ggggatgtcg ccgccgatgc cgacgaggtt gtgaagcccg
     4561 gcgtggagag aggacatgag cagggaggcc ggcacaccct tgcccgagac gtcggcgatg
     4621 gccacggcga ggcggttgtc gtcgagccgg atgcagtcga aatagtcccc gccgacctga
     4681 taggttggaa tattcaagcc cctgatctca atgtaatcgt tgccggggca gccacggggg
     4741 agcagccgct gctggatatc gcgagcgata ttgagctctt cttcgatgcg ctccttttcg
     4801 agtgcttcag cgaagaggcg ggcattttcg atggaaatca tggcaagatt gcacagggtg
     4861 gagagaaact cgatttcgtc gggctgaaag ggttgggcct tgattttttc accgacgacg
     4921 aagaggccgc gaatctcttc ctccatctgc atggggataa ccagtttgag gtgcatctcc
     4981 ctgaggagtt gcagtccggg tgcaggttcc tcctcgtcca cgttgagcac gcggtcgacc
     5041 cctgcgagag cggagatgaa gccgggggca ttgaggagct ggcagttggg gtcatccgga
     5101 cgcatccccc ggctggcgaa aagctgcaga gcatcatccc ggcgcaggta gatatagcag
     5161 cggttgacca ccagctcgcc catgaccgcg tagataagca ggttgacaat gcggtcgatc
     5221 tcgaggttcg cattgagttc cttgccaatc tcgaagaggg tgcgcagttc ctgaaccttt
     5281 ttatccagac ggcggttgac cagttcaagt ttctgataca tgagggcatt ttcgatggag
     5341 gaggcggcaa tattggagag cgaggtcagg tactcggtct cggcgggggt gaaaggtatg
     5401 ccgccgagtt tgctgcccag ccccatgatg ccgaccgtgc gctggacgct gataatcgga
     5461 atgaccaggt caatccggtt ctcctcgaaa aaagccttca gtttttcatc cgtctcacgg
     5521 atatcggcca cgacaaagga atcgggcagc tcacctgtgc agaagacctt tttacccatc
     5581 agggtgcgcg gcagcccctt gagagcggcg acctcgaagg cctggtgttc attaacgacc
     5641 aggacgatcc ctcggctgat catcatcctc cccatggggg tgaggagcag attgttgagg
     5701 gtggaacgca gctgcaagga cgagttcagg acctggctca tctcgaaaag ggactgcagt
     5761 tcgaccaggc gttcatcaag ttcgcgagcc accgcgctct gggagtccgg ttctttcacg
     5821 tcagggcggc cccgttcagt tgacaatttt tgagttcttg cgattgatca gatatttcac
     5881 catccggacc tgattgcgaa cgccgggttt gatgtcatag tcgacctcat ccatcagggt
     5941 gcgcatcaaa aagatgccaa gtccaccgac gcggagttcg gccagatact ccttcatatc
     6001 gggctgccgg atcttgtccg gattgaagcc cttgccatgg tcggtgacaa ccagagtaag
     6061 cttttggtag tcgataagta tggccacatc cagggtttta tcctttaccg tctcggtgta
     6121 ggcgtgcttc atcacattgg tgcaagcctc gtcgacggcc agttcgattt tcccgacatc
     6181 atcctccgca aagccgacct tttcggccac cttgccgaca aagaaacgga tgatctccag
     6241 attttcagtc tgactgggaa tgcgcagttt atattctatt ttggctttcg gcatatgggg
     6301 ttccgccttg tatacgttgg taacattcag gataagggga tactaagccc cttcatcgac
     6361 agagatccgt taccgcttca ctcactggag gaagcggcgg agttttgcgg gaaggtcgcc
     6421 agcgcttcct cctgcttgtc gaatatctgg tagatggtcg gaaaacccag gagatcgaaa
     6481 acccggtaaa ttttgggaga catcgcgcac atcttgatgt ctccattctt gctccggacc
     6541 tcctcgatga agcccataaa aacccccaga ccggcgctgc tgatataact cagctccttg
     6601 aaatcgacga ggatgttgac ctgttcctca tcgagcagct tctggatcgc ctcttcaaga
     6661 gtgggcgcgg tatgggcatc gaggaagccg attattttaa gtgtcgtcag attcacgcca
     6721 tccaggcgga ctactttgaa attttccatt cactttcctc catctatgct gaaatgctac
     6781 cctgtttcaa acagcttgca ggatgacgaa ggtcaaatca tcatgcgatc tcgcactgcc
     6841 gacaaagccg ctgatctcct caatgagcct tgcttgcagc tgttccggag gcagggcgtg
     6901 atggcgctgg aagagggaaa ccagtctggt ctcggtgaac tcttcgcccc gcgcattgcg
     6961 cgcctccaca gctccgtcag tgtaaaaaag aaaggtatc
//
LOCUS       DAOPWU010000174         6995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10745_length_6995_cov_4.742363, whole genome shotgun sequence.
ACCESSION   DAOPWU010000174 DAOPWU010000000
VERSION     DAOPWU010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10745_length_6995_cov_4.742363"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="PKM23_09550"
     CDS             complement(<1..1383)
                     /locus_tag="PKM23_09550"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91757.1"
                     /translation="MKKLFVLLILSAISALPLRAETPSVPAERIGRITGLIFDAATRE
                     ALIGANIQIVGTTFGAISDVNGAFRIERVPEGTIRLQVTMIGYKPIVEPDLIVSAVKP
                     LDVRIGLNQTVLEGASVTIKPNYFESVSDKPLSVQTQNYAEIRRLPGGLEDVVRAISI
                     LPGVAQVQNGRNDLIVRGGAPSENLYAIDGIEVPNINHFGTQGATGGPLSFVNLDYVA
                     NTTFSTGGFGVRFGDRLSSVTSIELREGRTDRWGGKATISASQFGLNLEGPLADKGSL
                     VFSARRSYLDFIFKAAGFAFVPEYWDFLIKGDYRLSDRDQLTLTALSAIDDVKLFNDT
                     AEKRYDNSRVIASDQYQAVAGLTWRRLYKAGYSTVTLSNNRFRYDLVQSDTSRNPIFI
                     NNSLERETLITANLVFKPAKSSEVTLGAQARAVFFESDIKLPPFVTPFGQLLEVDALL
                     DTTAVKSAAWL"
     gene            complement(1485..2786)
                     /locus_tag="PKM23_09555"
     CDS             complement(1485..2786)
                     /locus_tag="PKM23_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNY91758.1"
                     /translation="MNPQPLPHDLPLQLPLPEGLVKHLLVLLFLIHILFVNFMLGGQS
                     LAVILEIIGLRRKKYDRLAYEISSTVTVSKSLAVVLGVAPLLAINVAYTTYFYSSTVL
                     IGFAWLMIIIMASAAFLLTYLHKYTWESWGGSQRALHLTVGAAAMVLFLAVAMVFITN
                     INLMVLPAYWPRVAGFLSAVLLPSALLRYFHFILATLAISALFAAGWFGRKGYDLARL
                     PDFTRTEVIALFLRIAFIVTLLQFVIGPSLLLSLPVHGHSLAVWLLLLTGATIAGVMA
                     WIIYRELGRAPAVLGRSYLVMLTLLTFTALFMAYGRHYYRERAVNPHRQAMMAKTEAF
                     MWDAKAAQTRARMGMTREVYKSSGEKEFKANCAACHAENTTIVGPPLTEVRGLYAGNP
                     QNLIAWARAPQVKRGGAPMPSFNHLPEKVLQEIATWILEGK"
     gene            complement(2893..4302)
                     /locus_tag="PKM23_09560"
     CDS             complement(2893..4302)
                     /locus_tag="PKM23_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNY91759.1"
                     /translation="MDFPIFHLDFIGNRMLIAITAIVHVIINHPMAVGAIPMATLLEW
                     IAHRKKWSALDDLAYKITFVFFVITTSLGAMTGVGIWFTTALVNPDAIGSLIRVFFWA
                     WFSEWVVFFLEVVFIMIYFLTWKPWSGEKKRLHLGVGLFLSIFSWLTMAVITGILGFM
                     MNTGKWVPFSYEWVPESSMFTAFFNPLYLPQLIFRTAVAFLGAGLFFLFLIPFFKKLT
                     AEVRGRAVRPISLWVLAWLPVAIAGAIFYWQRIPVYMQANAPVALATQDFTRWYQTIL
                     WFLLGMAAIVPLAALWGVVKPKRLPAAALVIPFIAVATLLGTFERLREFVRKPWVIQG
                     YLYANGLRAQDYPLFKEEGVLSRATWVRHHQVTEENKLEAGGELFMLTCSRCHTVGGV
                     NSVRTRFQKLFPVQKWDEEQLAAYISTMHNIRIFMPPFPGSDAELKALSAWLAANQHY
                     AMRAEGGQTALLPSAPVLK"
     gene            4547..5479
                     /locus_tag="PKM23_09565"
     CDS             4547..5479
                     /locus_tag="PKM23_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNY91760.1"
                     /translation="MAQTRPGLFALMRAPFFSSILAPLFAGTLLAVIATGRFSPVGFA
                     VVLVMGLALHAATNVYNDIFDTLQGTDRINLHRNDFSGGSGFLLEHPHLLPGMYRIAR
                     VSLIVAAGAAMLLLFFTRHTLWPWLAGLYLLAAFFSKYYTAAPVKLAYRGLGELAVWF
                     AFGPMAILVAAVSQNTLFHPFIIAAMPITGLSTSSILWMGQLIDLQADSSSGKRGLVA
                     RTGSRLGRWGFLVIHLLLIANLIVLALIVLPQGWPLLAALLPYLLLPRTWKQVLRYHD
                     QPGELKAAAGWNVQIHLGMAVLLVLGLCAVLLLH"
     gene            5501..6502
                     /locus_tag="PKM23_09570"
     CDS             5501..6502
                     /locus_tag="PKM23_09570"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HNY91761.1"
                     /translation="MLTSIRTFLHPEGYHGKKRRPPFFEGWFYKCVDARGENRLAVIP
                     GIALHREPEQDHAFIQILDGRSGRSYYHRLQASQFSASPRSLEQCIGSNRFSRGGMAL
                     EITGEEWQLKGELCFGPFTPWPVTLRSPGAMGWYAWAPFMECNHGVVSFDHELGGKVI
                     LNGEEIDFNGGRGYIEKDWGRSFPQAYIWLQSNHFPVPGTSFMASVAIIPWLGRAFPG
                     FIIGLHHRHHLYRLATYTGALLEALEVGDAEILLQICDRQHRLSVRAERSATGLLHSP
                     TPQGMQGRIAETLGGTISLRLDRMDTRETILEQTGLHAGIDAAGDLPALLALLHRNS"
     gene            6699..>6995
                     /locus_tag="PKM23_09575"
     CDS             6699..>6995
                     /locus_tag="PKM23_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91762.1"
                     /translation="MPLFYRWTTVILPCLVAALLAGSGCEKQSTAPKKSAPKYQPITA
                     PLDPFAVNQRLAKSVNFGNALEAPNEGEWGLTLEARYFQLLDEAGFSGIRLPVRW"
BASE COUNT         1368 a         2092 c         2214 g         1321 t
ORIGIN      
        1 cagccaggcg gcgctcttga cggcggtggt gtcgagcagg gcatccactt cgaggagttg
       61 gccgaaggga gtaacaaatg ggggcagctt gatatcgctc tcgaaaaaga ccgcgcgcgc
      121 ctgggcgccg agggtcacct cgctgctctt ggccggttta aagaccaggt tggcggtgat
      181 cagggtttcc cgctccaggg agttgttgat gaagatggga ttccggctgg tgtccgactg
      241 gaccaggtcg tagcggaagc ggttgttgct gagggtgacc gtggagtagc ctgccttgta
      301 aagccgccgc caggtcagcc ccgcaaccgc ctggtactgg tcgctggcga tgacgcgcga
      361 gttgtcgtag cgtttctcgg cggtatcgtt gaagagcttg acgtcgtcga tagccgatag
      421 cgcagtcagg gtgagctggt cgcggtcgct gaggcggtaa tcgcccttga tgaggaaatc
      481 ccagtactcc ggcacaaaag cgaagccggc cgccttgaag ataaagtcca gatagctgcg
      541 ccgggcggag aagaccaggc tgcccttgtc ggcgaggggg ccttcgaggt tgaggccgaa
      601 ctgggaggcc gatatggtcg ctttgccgcc ccagcggtcg gtgcggccct cgcgcagttc
      661 gatgctggtc accgaggaga ggcggtcgcc gaagcgcacc ccgaagccgc cggtcgagaa
      721 ggtggtattg gcgacatagt caaggttgac gaaactgagg ggcccaccgg tcgccccctg
      781 ggtgccgaag tggttgatat tggggacctc gatgccgtcg atggcgtaga ggttttccga
      841 gggggcgccg ccgcgcacga tcaggtcgtt gcggccgttc tggacctggg ccacgcccgg
      901 caagatcgaa atggcgcgca cgacatcctc gagtccgccg ggcagacggc ggatctcggc
      961 atagttctgg gtctgcacac tcaggggctt gtcgctgacg ctctcgaaat agttgggttt
     1021 gatggttacg ctggcgccct cgagaacggt ctgattgagg ccgatgcgga catcgagagg
     1081 tttaactgcg gagacaatga ggtcaggttc gacgatcggc ttgtaaccga tcatggtcac
     1141 ttgcaggcgg atggtccctt cggggaccct ttcgatgcga aacgcgccgt tgacatcaga
     1201 gatggcgcca aatgtggtgc cgacgatctg gatattggcg ccgatcaggg cttcccgggt
     1261 ggcggcgtcg aagatgaggc cggtgatgcg tccgatgcgc tccgccggaa cggagggtgt
     1321 ttccgcccgc agcggcagtg cggatatggc cgatagtatc aagagtacaa acagcttttt
     1381 catttttcct cgattccgtg tcatgtgttt gtgtttatgt tcacagcggt taacagcgga
     1441 tcgaggtaaa atatttcatc atggtggcag agtccgccac ctggttactt cccttccagt
     1501 atccaggtgg cgatctcctg aagcaccttt tccgggagat ggttgaagga gggcatggga
     1561 gcaccgccgc gtttgacctg aggggccctg gcccaggcga tcaggttctg cggattgccg
     1621 gcatagagcc ccctcacctc ggtcaggggc gggccgacaa tggtggtgtt ttccgcatga
     1681 caggcggcgc agttggcttt gaattccttc tccccagagc ttttatagac ctcccgggtc
     1741 atccccatgc gggcgcgcgt ctgggccgct ttggcatccc acatgaacgc ctcagtcttg
     1801 gccatcatcg cctggcggtg ggggttcacc gcccgttcgc ggtaatagtg gcggccatag
     1861 gccataaaga gggcggtgaa ggtcagcagg gtgagcatga ccaggtagct gcggccgaga
     1921 acggcggggg cacggcccag ctccctgtaa atgatccagg ccattactcc ggcgatggtg
     1981 gcgccggtga ggaggagcag ccaaacggcc agagagtggc catgcacggg cagggagagg
     2041 agcagggacg gcccgatgac aaactgcagc agggtgacga taaaggcgat gcgcagaaag
     2101 agtgcaatga cctctgtgcg ggtgaaatcg ggcagccgag cgaggtcata gcccttgcgg
     2161 ccgaaccagc cggcggcgaa gagggccgat atggcgaggg tggcgaggat gaaatggaaa
     2221 tagcgcagca gagcgctcgg cagcagcacg gccgagagaa agcccgccac ccgcggccag
     2281 taggcgggca ggaccatcag gttgatattg gtgatgaaga ccatggcgac ggcgagaaag
     2341 agcaccatcg ccgcggcgcc gacggtcaga tgcagggcgc gctggctgcc tccccaactc
     2401 tcccaggtgt atttgtgcag ataggtgagc agaaaggcgg cgctggccat gatgatgatc
     2461 atcagccagg caaagccgat gagaacggtc gaggagtaaa aataggtggt ataggcgacg
     2521 ttgatggcga gcaggggcgc cacgcccagg accacagcca ggctcttgct gaccgtgacc
     2581 gtggaggaaa tctcataagc gagacggtcg tattttttgc ggcggaggcc gatgatctcc
     2641 aggatcaccg ccaggctctg gccgccgagc atgaaattga cgaagaggat gtgaatgagg
     2701 aagagcagca ccagcaggtg cttgaccagc ccttcgggca ggggcagctg cagagggaga
     2761 tcatgaggca gaggctgtgg attcatcgtc atatcctgag tgagcggtta ggcaaccatc
     2821 cgttgatttt gatcggcaca gaagggggcg cggcggccgg tttgatggcc caaccaccgg
     2881 ttgtgaggcc tcctacttga gcaccggtgc gctgggcagg agggcggtct gccctccctc
     2941 ggcgcgcatg gcgtagtgct ggtttgcggc cagccaggcg ctcagggcct tgagttcggc
     3001 gtcgctgccg ggaaatggcg gcatgaagat gcggatattg tgcatggtgc tgatataggc
     3061 cgccagctgc tcctcatccc atttctgtac aggaaagagc ttctgaaagc gggtgcgcac
     3121 gctgtttacc ccgccaacgg tgtggcagcg gctgcaggtg agcatgaaga gctcccctcc
     3181 cgcctcgagc ttgttctctt cggtcacctg gtggtggcgc acccaggtgg cgcgggagag
     3241 cactccctcc tccttgaaga gaggatagtc ctgggcgcgc agcccgttgg cgtagaggta
     3301 gccctggatc acccagggtt tgcgcacaaa ttcgcgcagc cgttcaaagg tgccgagcag
     3361 ggtggccaca gctatgaaag ggatgaccag ggcggcggcg ggcagccgct tcggtttgac
     3421 caccccccag agggcggcga gggggacaat ggccgccatg ccgagcagga accagaggat
     3481 ggtctggtac cagcgggtaa agtcctgagt cgccagtgcg accggggcgt tggcctgcat
     3541 gtagaccggg atgcgctgcc agtaaaagat cgcgccggcg atggccacgg gcagccaggc
     3601 cagcacccag aggctgatcg ggcgcacagc gcggccgcgc acctccgcgg tgagcttttt
     3661 gaagaagggg atgagaaaga ggaaaaacaa cccggcgccc agaaaggcca cggcggtgcg
     3721 aaagatgagc tggggcagat aaagcgggtt aaaaaaggcg gtgaacatgc tcgattcggg
     3781 cacccactcg taggagaagg gcacccattt accggtgttc atcataaaac cgaggatgcc
     3841 ggtgatgacc gccatggtca gccatgagaa gatgctgaga aagaggccga ccccgaggtg
     3901 cagccgcttc ttctcaccgc tccagggctt ccaggtgaga aaatagatca tgataaagac
     3961 cacctcgagg aaaaagacca cccattcgga aaaccaggcc cagaaaaaga cccggatcag
     4021 gctgccgatg gcgtcgggat tgaccagggc ggtggtgaac cagatgccga ctccggtcat
     4081 cgcaccgagc gaggtggtga tgacgaaaaa gacgaaggtg attttataag ccaggtcgtc
     4141 aagtgcagac cattttttgc ggtgggcgat ccattcaagc agggtcgcca tggggatggc
     4201 tccgaccgcc atgggatggt tgatgatgac atgcacgatg gcggtgatgg cgatgagcat
     4261 gcggttgccg atgaaatcga gatggaaaat ggggaaatcc ataacgcgcg tctccgtggg
     4321 gggtacggat gcctgtttca atccctaaag tataaagaaa aagaggatta ttggcaagaa
     4381 ctaatcgctt tttttgccat ttttcagcct aattctgcaa accggaaggg ctatctacag
     4441 cgcagcggca gaaaaagtcc ctgtacaatc aagtattcaa ctcaaggaat gcactccgga
     4501 attctgctgt tatgaagtga gtactcaaac caagaggagt gattttatgg cacaaaccag
     4561 gcccggtctc ttcgccctga tgcgggcgcc ttttttctcc tccatcctcg ctcccctctt
     4621 cgccggcacc ttgctggcag tgatcgctac cggccgcttc tcccccgtcg gattcgccgt
     4681 cgtgctggtg atgggccttg ccctgcacgc ggctaccaac gtctacaatg atatttttga
     4741 taccctgcag gggaccgacc ggatcaacct ccaccgcaac gatttcagcg gggggagcgg
     4801 cttcctgctc gagcaccccc acctcctgcc ggggatgtac cgtatcgccc gggtcagcct
     4861 gatcgtcgcc gctggcgcgg cgatgcttct cctcttcttc acccgccaca cgctctggcc
     4921 ctggctggcc ggactctacc tgctcgcggc ctttttcagc aagtattaca ccgccgcacc
     4981 ggttaagctg gcctatcgcg gacttggcga gctggcggta tggttcgcct tcgggccgat
     5041 ggccatcctg gtcgccgccg tgagccaaaa caccctcttc caccccttca tcattgccgc
     5101 catgccgatc accggactga gcacctcctc catcctctgg atggggcagt tgatcgatct
     5161 gcaggccgac agcagctccg gcaagcgcgg gctggtcgcc cgcaccggca gcaggttggg
     5221 ccgctggggc tttctggtca tccatctcct tctcatcgcc aacctgatcg tcctggccct
     5281 gatcgttctg cctcagggtt ggcccctcct cgcagccctg ctcccgtacc tcctcctgcc
     5341 ccgcacctgg aaacaggttc tgcgctatca cgaccagccc ggggagctca aggccgcagc
     5401 gggatggaat gtgcagattc atctcggcat ggccgtcctg ctggtcctcg gcctgtgcgc
     5461 cgtactcctg ttgcactaat ccttcaccct gcggagagcc atgctcacct ccatacgaac
     5521 gttcctccat ccggagggtt atcacggaaa aaagcgcagg ccgccctttt tcgagggctg
     5581 gttctacaaa tgcgtcgatg cccgcggtga aaaccgcctg gcggtgatcc ccggcatcgc
     5641 tctgcaccgg gagccggaac aggaccacgc cttcatccag atcctggatg gccgcagcgg
     5701 ccgcagctat taccatcgcc tgcaggccag ccaattcagc gcctcgcctc gcagccttga
     5761 gcagtgcatc ggttccaacc gcttcagccg tgggggcatg gctctcgaga tcacaggcga
     5821 agaatggcag ctcaaggggg agctctgttt cggccctttc accccatggc cggtcacgct
     5881 gcgctcccca ggcgccatgg gctggtacgc ctgggccccc ttcatggagt gcaatcacgg
     5941 ggtagtcagc ttcgaccacg aactcggcgg gaaggtgatc ctcaacggtg aggagatcga
     6001 ctttaacggc ggccgcggct atatcgaaaa agattggggc cgttccttcc cccaagccta
     6061 catctggctg cagagcaacc atttcccggt cccggggacc agcttcatgg cctcagtcgc
     6121 catcatcccc tggctgggca gagcctttcc gggcttcatc atcgggctgc accaccgcca
     6181 ccacctctac cgcctcgcca cctatactgg cgcccttctt gaggccctcg aggtcggcga
     6241 tgcagagatc ctgctgcaga tctgcgaccg ccagcaccgc ctctccgtcc gcgccgagcg
     6301 ctccgccacc ggtctgctgc actcgcccac gccgcagggc atgcagggcc gcatcgccga
     6361 aaccctcggc ggcaccatct cgctgcggct cgaccgcatg gacaccagag agaccatcct
     6421 ggagcagacc gggctgcacg ccggcatcga cgccgccggg gatctgcctg ccctgctcgc
     6481 gctcctgcat cgtaattcct agggtccgct cccaactgcc gccacagcgc ccagccctca
     6541 caagaacaca cctcacggaa gctggcgcta tcgcccggtt caaaagagtc ggagttgccg
     6601 cccttacggt gcattcccgc aagattcccg ttgccttttt cctccggatt gcctaatttg
     6661 gcaggtcaga gtgcccattc agcagccagg aaatgcccat gcctcttttt taccgttgga
     6721 ccaccgtcat cctgccctgt cttgtcgccg ccctgctcgc cggcagcggc tgcgaaaaac
     6781 agagcaccgc tcccaaaaag agcgcaccca aataccagcc gatcaccgcg cccctcgatc
     6841 ccttcgccgt caaccagcgc cttgccaaat cggtcaattt cggcaatgcc ctcgaggccc
     6901 ccaacgaggg ggagtggggc ctcaccctcg aggcgcgcta tttccagctc ctcgacgagg
     6961 ccggcttttc cggcatccgc ctgcccgtcc gctgg
//
LOCUS       DAOPWU010000175         6973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10793_length_6973_cov_5.567361, whole genome shotgun sequence.
ACCESSION   DAOPWU010000175 DAOPWU010000000
VERSION     DAOPWU010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10793_length_6973_cov_5.567361"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PKM23_09580"
     CDS             complement(<1..712)
                     /locus_tag="PKM23_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNY91763.1"
                     /translation="MKQGLFITIVMVVAFAIALVIFQFLPDYIKAGGILVPILIMMIL
                     MLLTFIIERLFALNKAKGKGSLTAFYRKVQQEIKAGNIDAAISACDKQRGTSASVLRA
                     GLEKYKLLKASGSHLSAKETMDEMQHSIEESMMLEVPLLERNLVGISTLASIGVLVGL
                     LGTTLGMIKAFKALAKSGAPDAIALSLGISEALINTAGGLITAILGIVAFNYFSTRID
                     NFTYMIDEATYSMVTTLSE"
     gene            complement(748..1113)
                     /locus_tag="PKM23_09585"
     CDS             complement(748..1113)
                     /locus_tag="PKM23_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY91764.1"
                     /translation="MAVRVMTMNNLDIAIVSVRGSLVGGEESDELKLTVADLFEQGNR
                     KLIIDLSGATYCNSLGIGTLVGLHTMYTRGMGQIKLCEMGKGIQNLFVITKLISVFDV
                     EDSRDEAIASFRTRESVHH"
     gene            complement(1360..2691)
                     /locus_tag="PKM23_09590"
     CDS             complement(1360..2691)
                     /locus_tag="PKM23_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY91765.1"
                     /translation="MSTPSAARVLVVDDELHILKTIGICLESIGFTTILTPRPQEALE
                     VARRERFDLAFIDLRMQPYDGMEMLERLHAIDADLTVVIITAHGSIDSAVEAVKKGAY
                     HYLQKPFDLKELQITAQKAWEHHQLTSVVRDLRERVIQQPDTGEFITRNQEMLALLDL
                     AGRIADSNISVLIEGESGTGKELIAHFIHNHSAREAKPFVKINCAALPEELLESELFG
                     HIKGAFTGATRDRQGRFEIADGGTIFLDEVAEISPSIQAKLLRVIQSREFERVGESVT
                     RKVDVRIIAATNRDLDEALREGSFRDDLFYRLNAVRLKATALRQRPEDIPLLVHHFIV
                     KFAKEAPPGISIEAMKALRLCRWFGNVRELENVMERAVLLAEGGTIEPAHLPEEVRRV
                     SESPVYALTLEEVEKRHIIQVLEHAQDYDEAARILGIDPATLWRKRKKYQL"
     gene            complement(2688..4526)
                     /locus_tag="PKM23_09595"
     CDS             complement(2688..4526)
                     /locus_tag="PKM23_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91766.1"
                     /translation="MPSLRFKIGAGYYTLVAIIVVLSIFAFVSFFRVNRAITPILKKH
                     TPTAIASQRMLKALDEQIQDQILMLRGDTELAYVSYQRSRDRFLNWYQHALAADLPQP
                     EKAILDSIIYTYKAYLTNSEAFYTLCKKNSPLSRPFHMESIVPVEQRLRSQCLHLIEV
                     TQALTTSTVELARDAMNRSIVLIAVAALLSIFFAFWSNAQVSRNVIRPASALARTIRL
                     ISRGQLNQKIDITTNDEFAELSSEFNKMTERLRTYEEMDIHQLIAEKVKSETIVASID
                     EPLIVTDAGHKVVLMNLAAAQMLGSPTNNLEGKPVEKVVADRKLRRLLMASATQLRET
                     AHSDFLLKIENSGSTTYYRPRQTTIVDETGQNQGIVTLFEDVTRFKDLERLKTEFIAT
                     VSHEFRTPLTSINMSIDILSQQLLGPVSARQAELLSNAKEDCGRLTKLVRELLDLSRL
                     EAGHHQMKMVPLSLEIIVQEALGPLRLPFLEKEIRLSSELAGLPPFLGDPQQLSYVLT
                     NLLSNALRHTPRGGEVCISATAGTEELRVAVADTGAGIPAEARETIFEKFVQVRKESE
                     TTPGSVGLGLAIARQVVQAHGGRIWVESEPGRGSTFYFTLPLTRSL"
     gene            4721..5782
                     /locus_tag="PKM23_09600"
     CDS             4721..5782
                     /locus_tag="PKM23_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNY91767.1"
                     /translation="MAATIYDVAKKAGVGIGTVSRAINNSPHINVGTKARILAIANEL
                     HYQPHALAQSLARKKTNTIASVVPFFTNYFYVELLKSVQRALSRSHYDLILFSVDRMN
                     RRDRTIDRVLSERRCDGVLIISLGVMDGYAEKFIAAGLPVVLIDHMHQALDSVAIANR
                     EGAFAAASHLIGLGHRRIGMINGHLSSYPAKLRLEGFRLALEMHGLDFDDRDLVICDA
                     KAGEHGFNEAAGYAAMQRLIAQSHPLPGALFVASDVQCLGVMRAAKEAGVRIPQDLAL
                     VGFDDIEFAKFVGLTTMRQPIAAMGRMAVTRLLKRIEGRENGDFHRELEAELIVRESC
                     GSRQAFHVRTESDTVSQQV"
     gene            5818..>6973
                     /locus_tag="PKM23_09605"
     CDS             5818..>6973
                     /locus_tag="PKM23_09605"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91768.1"
                     /translation="MMNKPLQTSLTWLVVLALLLPLLAFAGTTGKIVGKVKDDKTGET
                     LPGVNVVLEGTMLGAVTDYDGDLMIINVPPGTYTMQVKMIGYQPKSVSNVKVSVDMTT
                     TINVSLSQTVVDVGEQVTVVADRPIVQKDKTSSMAAVSSDEISAMPVQEVADVLELQA
                     GLVRDGVGGIHVRGGRSGEVAYWVDGIAATDGYSGNMGVQVENSSVQELQVISGTFNA
                     EYGQAMSAVVNIVTKEGGDKLHGEFSAYTGDYLSYDDDTYWVLEPNSDEISAIRSPQE
                     GTYAKTNPLRSFNPIYNVQGSLSGPLLKDKLTFFITGRYFSNDGYLYGKRWFTPQGLK
                     GDGELVSMNPFAKFSGQGKLAYRLNGNMKLTYTLMGNQNNFRWYDSYYKYN"
BASE COUNT         1350 a         2013 c         2177 g         1433 t
ORIGIN      
        1 tctcggagag cgtggtgacc atcgagtagg tggcttcgtc gatcatgtag gtaaagttgt
       61 cgatgcgggt gctgaaatag ttgaaggcga cgatgcccag gatagcggtg ataaggccgc
      121 cggcggtgtt gatcagggct tcggagatac ccagcgacag ggcgatggca tccggggcgc
      181 ccgacttggc cagggctttg aaggccttga tcatgccaag ggtggtgccg agcagaccga
      241 cgagcacgcc gatggaggcc agggtcgaaa tacccaccag gttcctctca aggaggggca
      301 cttccagcat catcgactct tcgatcgaat gctgcatttc atccatggtc tccttggcgc
      361 tcaaatggct gccggaggcc ttgaggagct tgtacttttc caggccggcg cgcaggacgc
      421 tggcggaggt gccgcgctgt ttgtcacagg cggagatggc ggcatcgatg ttgccggctt
      481 tgatctcctg ctggaccttg cggtaaaagg cggtcaggga gcctttgcct ttggccttgt
      541 tgagggcgaa cagacgctcg atgatgaagg tcagaagcat gaggatcatc atgatcagga
      601 tgggaacgag gataccacca gccttgatgt agtcgggcag aaactggaaa atgaccagag
      661 cgatggcgaa cgccacgacc atgacgatgg tgatgaagag accttgtttc atggtgcttt
      721 ctccttactt gtgatgatga tagtggatta gtggtgaacg gattcccggg ttcggaaact
      781 ggcgatggct tcatcccggc tgtcttccac atcaaagacg ctgatgagct tggtgatgac
      841 aaagaggttc tggatgccct tgcccatttc gcacaatttg atctggccca tgccgcgcgt
      901 gtacatggta tgcaacccca cgagtgtgcc gatgcccaga ctgttgcagt aggtggcgcc
      961 gctcaggtcg atgatgagct tgcggttgcc ctgttcgaag agatccgcga ccgtcaattt
     1021 cagctcgtcg ctctcctctc cgcccaccag ggagccgcgc acgctcacaa tggcaatgtc
     1081 aagattattc atggtcatga cccggactgc cacgtcaaaa cctcctcacg gacgatcacc
     1141 cctgcataaa atcactgctg ctattatatc aattattatg ccaaaatgca aagaataatt
     1201 tttgcataaa aaacaactca tcaccagccg gtcctgcgcc cggctcccgc cgtttcattg
     1261 caaaatgcaa tcccctggtg gcaacaaggc ctggaaaacg cgcttaagcg attgaatatc
     1321 aatccgatgg tggacaattg aaaaatgcat gacggggtat cagagctggt atttcttgcg
     1381 tttgcgccag agggtggcgg gatcgatccc gaggatgcgc gccgcctcgt catagtcctg
     1441 ggcgtgctcc agcacctgaa tgatatggcg cttctccacc tcctcaaggg tgagggcata
     1501 gaccgggctc tccgagacgc gccgcacctc ctcgggcaga tgggccggct cgatcgtccc
     1561 cccctccgcc agcagcacgg cccgctccat gacgttctcc agctcgcgga cattgccgaa
     1621 ccagcggcag aggcgcaggg ccttcatcgc ctcgatagaa atcccgggcg gcgcctcctt
     1681 ggcgaacttg acgatgaaat ggtgcaccag cagggggatg tcctccggcc gctggcggag
     1741 ggcggtcgcc ttgagacgca cggcattgag gcggtagaaa agatcgtcgc ggaaggagcc
     1801 ctcgcgcagg gcctcgtcga ggtcgcggtt ggtggcggcg atgatgcgca catccacctt
     1861 gcgcgtcacg ctctccccga cccgttcgaa ctccctgctc tggatcaccc gcagcagctt
     1921 ggcctggatc gagggggaga tctccgccac ctcgtcgaga aaaatggtgc ccccgtcggc
     1981 gatttcgaag cgcccctggc ggtcgcgggt cgcgccggtg aaggccccct tgatatggcc
     2041 gaacagttcg ctttcgagca gttcctcggg gagggctgcg cagttgatct tgacgaaggg
     2101 cttggcctca cgggcgctgt ggttgtggat gaaatgggcg atcagctcct tgccggtgcc
     2161 gctctccccc tcgatcagga cgctgatgtt gctgtcggcg atgcggccgg cgaggtcgag
     2221 gagggcgagc atctcctgat tgcgggtgat gaattcgccg gtatcgggct gctggatgac
     2281 ccgctcacgc agatcgcgta ccacgctggt gagctgatga tgttcccagg ccttctgagc
     2341 ggtgatctgc agctccttga ggtcgaaggg tttttgcagg tagtgatagg cccccttttt
     2401 aaccgcctcg accgcgctgt cgatggagcc atgggcggtg ataattacca ccgtcaggtc
     2461 cgcatcgatt gcgtgcagtc gttcgagcat ctccatgccg tcatagggct gcatgcgcag
     2521 gtcgatgaaa gccagatcga acctctcccg gcgggcgacc tcgagggctt cctggggccg
     2581 cggggtgagg atggtggtaa aaccgatcga ttccaggcag atgccgatgg tcttgaggat
     2641 gtgcagttcg tcatcgacga ccaggacccg cgctgccgac ggcgtgctca taggctcctc
     2701 gtcaagggaa gtgtgaagta aaaggtgctg ccccggccgg gttcgctctc cacccagatg
     2761 cgccccccat gggcctgcac cacctgtctg gcgatggcca ggcccaggcc gacgctgccc
     2821 ggtgtggttt cgctctcttt cctgacctgg acgaactttt caaagatggt ttcgcgcgcc
     2881 tccgcgggga tgcccgcgcc ggtatcggcg accgccaccc gcagctcctc cgtccccgct
     2941 gtggcgctga tgcagacctc gcccccccgg ggggtatggc gcagggcgtt gctgagcagg
     3001 ttggtcagaa catagctcaa ctgctgggga tcgcccagaa agggcggcaa cccggccagc
     3061 tcgctggaga gacggatctc cttctcaagg aagggcaggc gcagggggcc gagggcctcc
     3121 tggacgatta tttcaaggct gagggggacc attttcatct ggtggtggcc ggcctcgagg
     3181 cgcgaaaggt cgagaagctc gcgcaccagc ttggtgagcc ggccgcagtc ctctttggcg
     3241 ttgctgagca gttcggcttg ccgggctgaa accgggccga gcagctgttg gctgagaatg
     3301 tcgatggaca tgttgatcga ggtcaatggg gtgcggaatt cgtgtgagac cgtggcgatg
     3361 aattcggtct tgaggcgttc gagatccttg aagcgggtga cgtcttcaaa gagggtgacg
     3421 atgccctgat tctggccggt ttcatcgacg atggtggtct gacgagggcg gtaataggtg
     3481 gtgctgccgc tgttttcgat cttgagcaga aagtcgctgt gtgcggtctc gcgcagctgg
     3541 gttgcgctgg ccatgagcag gcggcggagc ttgcggtcgg cgaccacctt ctcgaccggt
     3601 ttgccctcga gattgttggt cgggctgccg agcatctgtg cggcggcaag attcatgagc
     3661 acgaccttat gccccgcgtc ggtgacgatg aggggctcat cgatgctggc gacgatggtt
     3721 tcggacttga ccttttccgc aatgagctga tggatatcca tctcttcata ggtgcgcagc
     3781 cgttcggtca tcttgttgaa ttcgctcgag agttcggcga attcgtcgtt ggtggtgatg
     3841 tcgatctttt ggttgagctg gccgcggctg atgagtcgga tggtgcgcgc cagcgccgag
     3901 gccggcctga tgacgttgcg cgagacctgg gcgttggacc agaaggcgaa aaagatcgag
     3961 agcagggcgg ccacggcgat gaggacgatg ctccggttca tggcgtcccg ggccagctcc
     4021 acggtgctgg tggtcagagc ctgggtgacc tcgatgaggt ggaggcactg gcttcgcagc
     4081 cgctgttcga ccggcacgat gctctccatg tgaaaggggc gggaaagagg gctgtttttt
     4141 ttacaaaggg tataaaaggc ctcggagttg gtcagatagg ccttgtaggt gtagataatg
     4201 ctgtccagta tggccttttc aggctggggc agatcggcgg cgagggcgtg ctggtaccag
     4261 ttgaggaagc ggtcgcggct gcgctggtag ctgacgtagg cgagttcagt gtcgccgcgc
     4321 agcatcagga tctggtcctg gatctgttcg tcgagggcct tgagcatgcg ctggctggcg
     4381 atagccgtgg gggtgtgttt tttcaggatc ggcgtgatcg cccggttgac gcggaaaaag
     4441 ctgacaaagg cgaagatgct gagcacgacg atgatggcca ccagggtgta gtagccggcg
     4501 cctattttga atctcaggct gggcatgatg ggcaatatac cggtttttct cgggttaatc
     4561 aagagataac tgtgagccgc caaaaagtcc ttgacaataa gttctttatt tagtaaaatg
     4621 attgtaaata ttttggaagc gcttcctgaa gcggcccttg caggggccga gcggggaagg
     4681 gctttttagc agcgcaggga gcaaccggcc ggagagcggt ttggcagcaa ccatctatga
     4741 tgtggctaaa aaagctggcg tggggatcgg cacggtctcc cgcgccatca acaacagccc
     4801 ccacatcaat gtcggcacca aagcccgcat cctcgccatc gccaacgaac tccactatca
     4861 gccccatgcc ctcgcgcaga gtctcgcccg caaaaaaacc aacaccatcg ccagcgtcgt
     4921 cccctttttc accaactatt tctacgtcga actcctcaaa agcgtccagc gcgccctctc
     4981 ccgttcccat tacgatctca tcctcttcag cgtggaccgc atgaaccgcc gtgatcgcac
     5041 catcgaccgc gttctcagcg aacgccgctg cgacggggtt ctgatcattt ccctcggcgt
     5101 catggatggc tatgcggaga aatttatcgc cgccggtctg cctgtggtcc tcatcgacca
     5161 tatgcaccag gcccttgatt cggtcgcaat cgcgaaccgg gagggggcct ttgccgccgc
     5221 cagccatctc atcggtctgg ggcaccgccg catcggtatg atcaacggcc acctttccag
     5281 ctacccggcg aagctgcgcc tcgagggctt ccgcctggcc ctcgagatgc acgggctgga
     5341 tttcgatgac cgcgatctgg tcatctgtga cgccaaggcc ggcgagcacg gctttaacga
     5401 ggcggccggc tatgcggcga tgcagcgtct gatcgcccag agccaccccc tgcccggcgc
     5461 cctcttcgtc gccagcgacg tccagtgcct cggcgtgatg cgcgcggcca aggaggccgg
     5521 ggtgcggatc ccccaggatc tggccctggt gggctttgat gacatcgaat tcgccaagtt
     5581 cgtcggcctc accactatgc gtcagcccat cgccgcgatg gggagaatgg ccgtcacccg
     5641 cctgctcaag cgcatcgagg gcagggagaa tggcgatttc catcgtgaac tggaggccga
     5701 actcatcgtc cgcgagagct gcggctcccg ccaggccttt catgtgcgga cagaatctga
     5761 tactgtgtca caacaagtgt gaaattcaac gttcctaacc agctgcaaag gaaaatcatg
     5821 atgaacaagc cgctgcaaac ctccctgacc tggctcgtgg tccttgctct gcttcttccc
     5881 cttctggcct tcgccggcac caccggtaag atcgtcggca aggtgaagga cgacaagacc
     5941 ggcgagaccc tgcccggcgt caatgtggtg ctggaaggca ccatgctcgg cgcggtcacc
     6001 gactacgatg gcgatctgat gatcatcaac gtccccccgg gcacctacac catgcaggtc
     6061 aagatgatcg gctaccagcc caagtccgtc tccaacgtca aggtctcggt cgacatgacc
     6121 accaccatca acgtcagcct cagccagacg gtggtcgatg tcggcgaaca ggtgacggtt
     6181 gtggccgata gaccgatcgt ccagaaggac aaaacctcct cgatggctgc cgtgagctcg
     6241 gatgagatct ccgccatgcc ggtgcaggag gtggctgatg tgctcgaact tcaggcgggc
     6301 ctggtccgcg acggggtcgg cgggatccac gtccgcggcg gccgctccgg cgaagtcgcc
     6361 tactgggtcg acggcatcgc tgccaccgat ggctactccg gtaacatggg ggtccaggtc
     6421 gagaactcct cggtccagga gctccaggtc atcagcggca ccttcaacgc cgaatatggc
     6481 caggcgatgt cggctgtggt caacatcgtc accaaggagg gcggagacaa gctgcacggc
     6541 gagttctccg cctataccgg cgattacctc agctatgacg acgacaccta ctgggtgctc
     6601 gagcccaact cggatgagat ctcggccatc cgttctcccc aggagggcac ctacgccaaa
     6661 accaatcccc tgcgcagctt caacccgatc tacaatgtgc agggcagcct gagcggcccg
     6721 ctcctcaagg acaagctgac cttcttcatc accggccgct atttcagcaa tgacggttat
     6781 ctctacggca aacgctggtt cactccgcag gggctcaagg gcgatggcga actggtgtcc
     6841 atgaatccct tcgccaagtt cagcggccag ggcaaactgg cctatcgcct caacggcaac
     6901 atgaagctga cctataccct gatgggcaac cagaacaatt tcaggtggta cgacagctat
     6961 tacaaataca atc
//
LOCUS       DAOPWU010000176         6941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_10858_length_6941_cov_4.890357, whole genome shotgun sequence.
ACCESSION   DAOPWU010000176 DAOPWU010000000
VERSION     DAOPWU010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10858_length_6941_cov_4.890357"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..4120)
                     /locus_tag="PKM23_09610"
     CDS             complement(50..4120)
                     /locus_tag="PKM23_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNY91769.1"
                     /translation="MLLVFALFLAMAFAPAAEAGQVVLPSKDNVLMQTNGPGSEFDDG
                     DYWTHPDYGNSPHIFWLEIPAGVNPDFMINLQLWDPESFSNNPESATDSDEQKGNGWE
                     DTEFALYAPDGSLIVSRTYTGGDVTTSQQWVSFHALRAGDFGPGMYKITAQVAGDDEN
                     GYKIGVEDGNADKLTANGNEIKLYAQRTAFQFVGEGDLTNTFWFNVGKRDSLRLLVFD
                     LEKDGCALKYISPSGVEYAGTLTEDGMWNSNPPSSHLPLQGGSDIIVNPEAGWWQAVV
                     TTYHPSPVVRQGNQFIFWPDDLLFNGPPTIHRGIVGDRVWLDANQNGLQDADETGIAN
                     ATVRLLTGETNMAIRTTTTDLDGIFTFKDVPAGNYKLEYQLPQYFFFTAPRVGSDREV
                     DSDADPFTGRTGVFFLQANEARFNLDAGMIPKHLSNLSVTKNVEGGDLELVPGEELLF
                     TITVTNNGPHDAANVRVVDNLPAALELLSVDRHYDAGPSPLLWIETLLPAGESRTYQV
                     RARATSVLGELENCAWVSSPNKDVDLIDNSSCVIVRVQQEYPNASGIKIGDRVWRDSN
                     RDGLQDENEPGMEGITVHLLSGVDQTLVQSDLTDADGLYQFADMPAGSYMIEFVLPEG
                     YAWTIPNQDAADERDSDVDQVNGRTPALFVLNGASYAMWDAGLTPYEPPQVSNIRIGD
                     LVWQDSNRNGLQDPGEPGMEAITINLLSGADESLVMTGQTDTEGRYLFADMAPGSYIL
                     QVIQPAGFDYTLPLQGDDRTVDSDILPESGKSQAFSVLSNHSYPGVDAGFAPFQPPVV
                     SNITIGDRVWNDANRNGLQDDGETGMAGLFVNLLSGIDQSLVAKDTTDAEGLYLFKDM
                     APGAYMIEVVQPAGYIFSLLKQGVDTSVDSDIIPESGRSEAFDVVANTAYLTIDAGLA
                     AYVPPVNSHITIGDRVWNDLNKNGQQDDGEPGMAHVTVRLVTGADDAAVSATTTDDNG
                     AYLFADMAPGSYRVIFTLPAGFHFTAWNSGDDAADSDANPVNGRTDQFTVASHTAYPS
                     WDAGVVENAESDLEIAKVIEDSRSYIYRGDSLTFVITLTNHGPDDAHGITLIDNLPDG
                     LEFIEATRTQDEGPNPIIWREEVLPVGASVSYRVRMRTTEELGGMDNCVTVSSLSFDP
                     DLANNIACAQVHILVPVELSSFNARSVQGRVILNWVTQSETENLGFHVYRSESENGTY
                     VRVNNEMIQGAGTTSSVRSYQYIDERELKPSTDYFYKLVDVDYKGRLNTHGPVSAVVS
                     VPTEHVLEQNYPNPFNPETRISFSLKEAGRVTLTVFNVRGESVRTLVAGQLNAGAHMV
                     VWDGRNDYGQPLPSGMYIYTMRINNFEEKRTMMFLK"
     gene            4578..5123
                     /locus_tag="PKM23_09615"
     CDS             4578..5123
                     /locus_tag="PKM23_09615"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNY91770.1"
                     /translation="MYTIKFIEGEKIYLRPYAESDLDLVQCGKNDPLVRETLFLAFPQ
                     TLEQVRAEMNSWSTTRETVLFTICSREKDKPVGQTAFVRIDLVSRAAVFYLALYDSGE
                     WSRGYGGEATRMMIDYGFNILNLNRIQLHVSCENEHAVSAYKKAGYSIEGTLRQAMYH
                     HDRYVDFYVMGILREEYYRRG"
     gene            complement(5208..5717)
                     /locus_tag="PKM23_09620"
     CDS             complement(5208..5717)
                     /locus_tag="PKM23_09620"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNY91771.1"
                     /translation="MKKHDSNLVLGLILILIGAGLLFKRMGFFDFGWEESYPIALLLL
                     AAMAIVSVAKGDRSQAFWACFLLICGLFTFLKNYGIVDSFWSVNLWSILLLAFGLSFF
                     ALYASKPKDWGVLIPGFILTTFGGVAILDDLHVDWFRISYLFDYWPLMLIAIGIAIIF
                     STLRRRPTN"
     gene            complement(5872..6555)
                     /locus_tag="PKM23_09625"
     CDS             complement(5872..6555)
                     /locus_tag="PKM23_09625"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNY91772.1"
                     /translation="MKKEKEALPQNLSDPNLWREQRLAMVDRQIRARGVQDSSVLAAM
                     EFVPRHLFVPEGERHLAYEDHPLPIGYEQTISQPFIVAYMTEKLGIRPGDKVLEIGTG
                     SGYQAAILSRLARVVYTIEIVEPLCREAAATLGRLGFSNVEVRCGDGFVGWPEEAPFD
                     AIMLTASPESIPNPLVEQLAPGGRMILPLGGHYQELVLIQRSLEGQLSRRELIPVRFV
                     PMTGEAETR"
     gene            complement(6608..>6941)
                     /locus_tag="PKM23_09630"
     CDS             complement(6608..>6941)
                     /locus_tag="PKM23_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91773.1"
                     /translation="LVLTPFFGLLSARPWPPLFYAALLAVSGLAGALFPLLSGLHARC
                     GGSGRWGSVNGAELAGGAAGALLIGGLWVPLFGFARTLGLVAAANLAGVLAIALCARP
                     HDKENRCE"
BASE COUNT         1334 a         1924 c         2300 g         1383 t
ORIGIN      
        1 aatctacgaa atagccggat tttcaccaat acctaccgga aaattttcct tatttgagga
       61 acatcatggt gcgcttttct tcaaagttgt tgatgcgcat cgtatagatg tacatgccgc
      121 tcggcagggg ctgaccgtag tcgttgcggc cgtcccacac caccatgtgc gccccggcat
      181 tgagctgccc ggcgaccagg gtgcgcaccg actcaccgcg gacgttaaag acggtcagag
      241 tgacgcgacc cgcctccttg aggctgaagc tgatccgggt ttcgggattg aacggattgg
      301 gatagttctg ttccagaaca tgttccgtcg gaaccgagac taccgcgctg accgggccat
      361 gggtgttgag ccggcccttg taatcgacgt cgaccagctt gtagaaatag tcggtcgagg
      421 gcttgagctc ccgttcgtca atgtactggt agctccgcac cgatgaggtg gtgccggcgc
      481 cctggatcat ctcattgttc acccggacat aggttccgtt ttcgctctcg gagcggtaca
      541 cgtggaagcc caggttttcg gtttcggact gggtgaccca gttgaggatg acccgtccct
      601 ggacgctccg tgcgttgaac gaggagagct cgaccgggac cagaatgtgg acctgggcgc
      661 aggcgatgtt gttggcgagg tcgggatcga aggagaggct gctcaccgtg acgcagttgt
      721 ccattccgcc cagttcctcg gtggtgcgca ttctgacgcg atagctgacc gacgcgccga
      781 ccggcagaac ctcttcccgc cagatgatcg gattgggccc ttcgtcctga gtgcgggttg
      841 cttcgatgaa ctcgaggccg tccggcaggt tgtcgatgag ggtgatgccg tgggcgtcgt
      901 ccgggccgtg gttggtgaga gtgatgacga aggtcaggga gtcgccgcgg tagatatagc
      961 tgcggctgtc ctcgatcacc ttggcgatct cgagatcgga ttcagcgttc tcgaccacgc
     1021 ccgcatccca ggagggatag gcggtgtggc tggcgaccgt gaattgatcg gtgcggccgt
     1081 tgaccggatt ggcatcggag tcggctgcat catcgccgct gttccaggcg gtaaaatgga
     1141 atcccgccgg caaggtgaag atgacgcggt agctgcccgg tgccatgtcc gcgaagagat
     1201 aggcgccgtt gtcgtcggtg gtggtcgcac tgacagcggc atcatcagcg ccggtgacga
     1261 gacggactgt aacatgagcc atgcccggct cgccatcatc ctgctggccg ttcttgttga
     1321 gatcgttcca gacccggtcg ccgatggtga tatggctgtt aaccgggggc acataggccg
     1381 ccaggccggc atcgatggtc aggtaggcgg tattggcgac cacatcaaag gcttcactgc
     1441 ggccgctttc ggggatgatg tccgagtcga cggaggtatc gacgccctgt ttgagcaggg
     1501 agaagatgta accggccggc tggaccacct cgatcatgta ggcgccgggg gccatatcct
     1561 tgaacaggta gagaccttcg gcgtcagtgg tgtctttcgc gaccaggctc tggtcgatcc
     1621 cgctgaggag attgacgaag aggccggcca tgcccgtctc gccgtcatcc tgaaggccat
     1681 tgcggttggc atcattccag acccgatcgc cgatggtgat gttgctgacg acagggggct
     1741 ggaagggagc gaaaccggcg tcgacgcccg gatagctgtg gttggagagg acgctgaaag
     1801 cctggctctt gccgctctcc gggaggatgt ccgagtcgac ggtgcggtca tcgccctgca
     1861 agggcagggt atagtcaaag ccggcgggct ggatgacctg gaggatatag gagccggggg
     1921 ccatgtcggc gaagagatag cggccttcgg tgtcggtctg tccggtcatg accaggctct
     1981 catcggcgcc gctgagcagg ttgatggtga tcgcttccat tcctggttcg ccgggatcct
     2041 gcaagccgtt gcggttgctg tcctgccaga ccagatcgcc gatgcggatg ttgctcacct
     2101 ggggcggctc atagggggtc agtccggcgt cccacatggc atagctggcg ccgttgagga
     2161 caaagagcgc cggggtgcga ccgttgacct ggtcgacatc ggagtcgcgc tcatcggcgg
     2221 cgtcctgatt cgggatggtc caggcatagc cttccggcag cacgaattcg atcatatagg
     2281 agccggcggg catgtccgca aactgataaa ggccgtccgc atcggtgaga tcggactgga
     2341 ccagggtctg gtccacgccg ctgaggagat ggacggtgat gccctccatg ccgggttcgt
     2401 tctcgtcctg gaggccgtcg cggttgctgt cacgccagac gcggtcgccg attttgatgc
     2461 cgctggcgtt cggatactcc tgctgcacgc ggacgatgac gcagctcgag ttgtcgatca
     2521 ggtccacatc cttgttcggg ctggagaccc aggcgcagtt ctcgagttca cccaggacgc
     2581 tggtggcgcg ggcccggacc tggtaggtgc gggattcccc ggcgggcagc agggtctcga
     2641 tccagagcag cgggctgggg ccggcgtcgt agtggcgatc caccgagagc agctcgaggg
     2701 cggcgggcag attgtcgacg acacggacgt tggcggcgtc atggggtcca ttgttggtga
     2761 ccgtgatggt gaagagcagc tcctcgcccg gcaccagttc aagatccccg ccctccacgt
     2821 ttttggtgac cgagaggttg gagaggtgtt tcgggatcat gccggcgtcg agattgaagc
     2881 gggcctcatt ggcctggagg aaaaagaccc cggttctgcc ggtgaaggga tccgcatcgg
     2941 aatccacctc gcggtcgctg ccgacgcgcg gagcagtgaa gaagaaatac tggggcagct
     3001 gatattccag tttgtaattg ccggccggga catccttgaa agtgaagatg ccgtccaggt
     3061 cggtggtggt ggtgcggatc gccatgttgg tttcaccggt gagcaggcgc acggtggcat
     3121 tggcaatgcc ggtctcatca gcgtcctgca gaccgttctg attggcgtca agccagaccc
     3181 ggtcgccgac tatgccgcgg tggatggtgg gcgggccgtt gaagagcagg tcgtccggcc
     3241 agaaaatgaa ctgattgccc tgacgcacga ccggcgaggg gtgataggtg gtgaccaccg
     3301 cctgccacca gccggcttcg ggattgacga taatatcgct gccgccctgc aggggcagat
     3361 gggaggaggg gggattgctg ttccacatcc cgtcctcggt cagggtgccg gcatattcga
     3421 ctccgctcgg gctgatgtat ttgagggcgc agccgtcctt ctccagatcg aaaacgagga
     3481 ggcgaaggga gtcgcgcttg ccgacattga accagaaggt attggtcaga tcgccctcgc
     3541 cgacgaactg gaaggcagtg cgctgggcat agagcttgat ctcattgccg ttggccgtca
     3601 gcttgtccgc attgccgtcc tcgacgccga tcttgtagcc gttctcatca tctccggcca
     3661 cctgagccgt gatcttgtac atgccggggc cgaaatcgcc ggcacgcagg gcgtgaaagg
     3721 agacccactg ctggctggtc gtcacatccc cgcctgtgta ggtgcggctg acgatgaggg
     3781 agccgtcggg ggcgtagagc gcaaactccg tatcctccca gccattgccc ttctgctcat
     3841 cagagtcggt ggcgctctcc ggattgttgc tgaaggactc ggggtcccag agctgcagat
     3901 tgatcataaa atcgggattc actccggcgg ggatctccag ccagaagatg tgcggggagt
     3961 tgccatagtc tggatgggtc cagtaatccc cgtcatcgaa ttcggaaccg ggaccattgg
     4021 tctgcatcag gacgttgtcc ttgctcggca gcaccacctg tccggcttca gccgcaggtg
     4081 cgaatgccat ggcgaggaac agcgcgaaaa ccagaagcat gagcccggct ctgatgccgt
     4141 gttgcctatt catttgctcc atttcaagtc tctcctatct gtcattgtat gcgctttgct
     4201 gcttgacgca ggctgtcatg aagaagagcc gcctgcactg ctcttttcag gtaaatctga
     4261 tcaccgcctc cctgtccggc acctcctttc gttgttcaac tcgcaaaacc tatatgattt
     4321 cagttacaca tgtcatctat ctttgtcgaa aaccggggct gcagcaggct ttttagagcc
     4381 attcaagata agtataatca acaggataaa ttgtgcaacg cgcgcccttt caccaggcta
     4441 ttgctcaaaa agcatgccat gatggcgcgg cagtcctgcg gataaaaaat tggtgcaaaa
     4501 aaggcgggaa atctgtacat tttccagatg ggcgcctggc ctgtcaccga ttcctgcatc
     4561 ctgtggaggg agataccatg tacacgatca agtttataga aggggagaag atctatctgc
     4621 gcccctacgc ggagagcgac ctcgatctgg tccagtgcgg caaaaacgat cccctggtgc
     4681 gcgagaccct cttcctggcc ttcccccaga ccctggaaca ggtccgggcc gagatgaaca
     4741 gctggagcac caccagggag acggttctct tcaccatctg cagcagagag aaggacaagc
     4801 cggtggggca gaccgccttc gtgcgcatcg acctcgtcag ccgtgcagcg gtcttttacc
     4861 tggccctcta cgacagcggg gagtggtcgc ggggctacgg cggggaggcg acgaggatga
     4921 tgatcgacta cggctttaac attctgaatc ttaaccgcat ccagctccac gtcagctgcg
     4981 agaacgagca tgccgtctcc gcctataaaa aggcgggcta ctctattgaa ggcacgctgc
     5041 ggcaggcgat gtatcatcac gaccgctatg ttgatttcta tgtgatgggg attctgcgcg
     5101 aggagtatta ccgcaggggg tgaggcgagg cctcaggggc ataaaaaaag ccgcctcacg
     5161 gtgcgggaga ggcggcagct ggaaacagga gggtcggcaa gaacggacta gtttgtcggc
     5221 ctgcggcgga gggtgctgaa gatgatggcg atgccgatgg cgatcagcat gagcggccag
     5281 tagtcgaaga gatagctgat cctgaaccag tcgacatgca ggtcatccag gatggcgacg
     5341 ccgccgaaag tggtgaggat gaatccgggg atgaggacgc cccagtcctt gggcttgctg
     5401 gcatagaggg cgaaaaaact gaggccgaag gcgagcagga ggatgctcca gagattgacg
     5461 ctccagaatg aatcgacgat gccgtagttt ttcagaaaag tgaagaggcc gcagatgagc
     5521 aggaaacagg cccagaaggc ctggcttctg tctcctttgg ccaccgagac gatcgccatg
     5581 gccgccagga gcagcagggc gatcggatag ctctcctccc agccgaaatc gaaaaacccc
     5641 atgcgcttga agagcagacc ggcgccgatg aggatcagaa tgaggcccag gaccaggttt
     5701 gaatcgtgct ttttcatgag aattcccttt gtcagctgtg atacgggatg ttcctgccct
     5761 tattaacgta aatccgcagg gattgtttca ctgctctctc ttttcctctg ccgtccgctc
     5821 catttccgcg gctgcggcct cgtcgccgcc ctcacacctc acatccgact cttacctggt
     5881 ctcggcctcc ccggtcatcg gcacaaagcg caccgggatg agttcccggc gtgagagctg
     5941 cccctcaagg ctgcgctgga tgagaaccag ctcctgatag tgtcccccaa gggggaggat
     6001 catgcggccc ccgggggcaa gttgttcgac caggggattg gggatgcttt caggtgaggc
     6061 ggtgagcatg atggcgtcga agggggcctc ttcgggccag ccaacaaagc cgtcgccgca
     6121 gcgcacctcg acgttgctga agccgaggcg gcccagggtg gcggcggcct cgcggcagag
     6181 gggttcaacg atctcaatgg tatagaccac ccgggcgagg cggctgagga tggccgcctg
     6241 atagccggaa ccggtgccga tctccagcac cttgtcgccg ggcctgatgc cgagcttttc
     6301 ggtcatgtag gcgacgataa agggctggga gatggtctgc tcataaccga taggcagggg
     6361 atggtcctcg taggcgaggt ggcgttctcc ttccgggaca aagaggtggc ggggcacgaa
     6421 ttccattgcc gccagcacgc tggagtcctg cacgccgcgg gcgcggatct gccggtcgac
     6481 catggccagg cgttgctcgc gccagagatt agggtcagaa aggttctggg gaagggcttc
     6541 tttctccttt ttcatacagg tgctcccgga taaaaaaaag agggcgagga ttgcggcgag
     6601 tggtttgtta ttcacaccgg ttctccttgt catgcggccg ggcgcagagg gcgatggcga
     6661 gcactccggc cagattggcg gctgccacca gcccgagggt gcgggcgaag ccgaagaggg
     6721 gcacccagag gccgccgatg agcagggcgc ctgcggctcc gccggccagc tcggcgccgt
     6781 tgacgcttcc ccagcgcccg ctgccgccgc agcgggcgtg gaggccgctg agcaggggaa
     6841 agagcgcccc ggcaaggccg ctgaccgcga ggagtgcagc atagaagagc ggcggccagg
     6901 ggcgggcgct gagcaggccg aagaaggggg tgaggacaag g
//
LOCUS       DAOPWU010000177         6808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_11154_length_6808_cov_5.332149, whole genome shotgun sequence.
ACCESSION   DAOPWU010000177 DAOPWU010000000
VERSION     DAOPWU010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11154_length_6808_cov_5.332149"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..547)
                     /locus_tag="PKM23_09635"
     CDS             complement(203..547)
                     /locus_tag="PKM23_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY91774.1"
                     /translation="MSLQLSTEQRGNVAVVGITGDVDLYSSPQLRKEILKLVNARDAR
                     LLVDLGKVTYMDSSGLATLIEGLQHINRNNGRMAVTGLRDAVKEIFELTRLDTVFSIY
                     PDTETALEWVAS"
     gene            complement(544..1035)
                     /locus_tag="PKM23_09640"
     CDS             complement(544..1035)
                     /locus_tag="PKM23_09640"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91775.1"
                     /translation="MELERKIARLFFTIRQFKPRWLPGKSIRISINSDPVYLRVLRAA
                     MDEIAALAGFSRKECAKIILAVDEACSNVIRHAYHDVAGQPIYITCRITPIKLEIVIV
                     DLGEPADIKSIKPRPLDELRPGGLGVHIIRTVMDEVHYENLAKIGNRLIMAKFLPREE
                     GAL"
     gene            complement(1137..3842)
                     /gene="polA"
                     /locus_tag="PKM23_09645"
     CDS             complement(1137..3842)
                     /gene="polA"
                     /locus_tag="PKM23_09645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNY91776.1"
                     /translation="MPQSADKKRLFLIDGAALAYRTYFAFARNPLVNSRGEQVSVIFG
                     FSRILLNLLDQEKPDLFAVVFDSPEPTFRHEIYTEYKAQRQRMPDDMAEQLPRLEQLM
                     GALDLPFIRKPGYEADDIMGTLARAAAASGYQTVLVTGDKDLMQLVDDHTLIYYPHRA
                     GESIEWLDADGVTRKMGVPPSQIIDYLALCGDASDNIPGVPGIGPVGALALLKRFGSL
                     EALLEQVDAIESKKQRTALQENADLARLSRTLATIHRDVPLEIGIETLHLRPIPAEKA
                     AAFFREMDLPSLVDRFSSRTRAVNRYQLVGDLKSVTELAERLRSCGAFALDTETTSVD
                     PMRAELVGLSFSCAEGEAWYIPVKGPADLTAGQQPLPTGEVLKILAPLLSDPAIKKCG
                     HNAKYDLLVLARHGVEVAGLASDTMVADYLLNPSGFQHNLDAVSLEHLGLKKIPTSQL
                     LGTGRNQRTMDLVPIEEVSEYACEDADYTWRLQQVLMPKLDAFGLRPLLEEVEVPLIH
                     VLMVMERNGVALDELYLAAMSGELEQELGVIEKSIYELAGRKFNINSPKQLGEILFSE
                     LKLPSARRTKTGFSTDVGVLEELAGAHPLPQRILDYRQLAKLKSTYVDALPRLINPAT
                     GRVHTSFNQTVAATGRLSSTEPNLQNIPIRTEVGRRIRRAFIPGDEGHLILDADYSQI
                     ELRIMAHLSGDRTLRDSFTAGEDVHARTAALVFKVPQAEVTPEQRRRAKEVNFGIMYG
                     MGAYGLASRLGLSNEEAEQFIQAYFASYPGVQEYMLRTVREARQNGYVTTLLGRRRMI
                     PEIASDNRRVREFAERTAINTPIQGSAADLIKVAMIRIQKRLEEEKSAARMIMQVHDE
                     LVFEVPRADAEALREMVRIEMEGAIRLDVPIRVETGVAGNWLEAH"
     gene            4067..4408
                     /locus_tag="PKM23_09650"
     CDS             4067..4408
                     /locus_tag="PKM23_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNY91777.1"
                     /translation="MSQVPPQQLNIELGEKEAEGIYSNLAVISHSPAEFVIDFTRMVP
                     GLPKTRVYARIIMTPQHAKSLLNALKENIAKYESQFGEIKTYSEPVRESTPMGFHADQ
                     AGSPQEKQNPR"
     gene            4408..5001
                     /locus_tag="PKM23_09655"
     CDS             4408..5001
                     /locus_tag="PKM23_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91778.1"
                     /translation="MRLRAICIWALFFAAGALAATPLNRGSLSLQTGVWKPGTLDSDP
                     GKPFTPVEGTGLCWGVGLCSPHAAGFALEMNLWQWERQLQGSEAEPVASRLVNLAFDL
                     KYQLLGATAIRPFVLYGGAALYGREDPASPPPGIGSELEFAGYALNFGAGFDFILSRH
                     LGLSAAYQYLYVDMRKEIGATTRYSGPKVTLKLLYLF"
     gene            5340..>6808
                     /locus_tag="PKM23_09660"
     CDS             5340..>6808
                     /locus_tag="PKM23_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNY91779.1"
                     /translation="MTAIKNRPLSAWLVGIGIIIAAWAPGLHAQWKIMPIGDSITDGV
                     GSTGAGYRSQLYDKLQGVNFSLVGPNGAAPYNGHFTRGAKIEEFYTGGYGTGIRDIAS
                     SMNTYQPDIILIHLGTNNMNNDDAAPYSENAGISMLPTSSGKLAQLLAYASQWANGTR
                     GDFLKRIIVCKIVPRLVNGSPDPKIADFNAEIDRMFFENAPGIVVSKVTLADMNSVVP
                     LSDLGDGTHPNDTGYAKMATEFNRILRGVIAGDRSAPASISWLGADAISGSTAGLKWY
                     STGDDGTNGQANLYEMRYSTFGLDAANFTQGILVSLPRPQSAGNIETASVSGLVPGIS
                     YFFGIRAYDEMNNRGPVSIGPSVDMADTASTEYCDDFEDPLAADWDLNPAYRIDVTRY
                     ELVNSATSSGWQYLAAYKAARYAPAVRGVRAAMQWSELADDRGINASGLAMMLNGPSQ
                     QASGYLVRVRDRVVYLNEIVNGGVPTGNLATAGFPPTAP"
BASE COUNT         1274 a         2219 c         2045 g         1270 t
ORIGIN      
        1 catccgcgtc atctggacga aagcggagcc ccagcggatg cgatggccgc gaaagggcgc
       61 gaccgcgagc cagtagaggg aaagggagac gaagcggccg aagcgcgcca gctgcacgag
      121 ggcgccggcg agaggatgcc agaaccggcc ggagatgtcg gcgcgtacgc tcaggcctgc
      181 cccccggccg cgggtgataa tgtcatgagg ccacccattc gagtgcggtt tcggtatcgg
      241 gatagatgct gaagacggta tccagacggg tgagttcaaa gatctccttg accgcatccc
      301 gcagtccggt cacggccatg cgcccgttgt tgcggttgat atgctggagc ccctcgatca
      361 gggtggccag ccccgagctg tccatgtagg tcaccttgcc cagatcaacc agcagcctgg
      421 cgtcgcgggc gtttaccagc ttgaggatct ccttgcgcag ctgtggagag gagtaaagat
      481 caacatcccc ggtgatgccg acgacggcca cgttcccccg ctgctctgtg cttagctgca
      541 gactcataat gccccctcct ccctgggcag aaactttgcc atgatcaaac ggttgcctat
      601 tttggccaga ttctcatagt gcacctcatc catcaccgtg cggatgatgt gcacccccag
      661 accgcccgga cgcagctcat cgagaggccg cggtttgatc gacttgatgt ccgccggctc
      721 gcccagatcg acgatgacaa tctccagctt gatcggggtg atccggcagg ttatatagat
      781 gggttggccc gccacatcgt gataggcgtg gcggatgaca ttggaacagg cctcgtccac
      841 ggccaggatg attttggcgc actccttgcg cgaaaagcca gccagggcgg cgatctcatc
      901 catcgctgcc cgcagcaccc tgagatagac cggatcgctg ttgatcgaga tccggatgct
      961 cttgcccggc agccagcggg gcttgaactg ccggatggtg aaaaagagcc tggcgatttt
     1021 gcgttctagt tccatgcgat cttccatcct gtggctcggt tcgtttcaga ccgcgctgcc
     1081 caccctcttc ccgtccccac ccggaagcaa atttttctct gctgcggccc gggcccctaa
     1141 tgggcctcga gccagttgcc cgccactccg gtttcgaccc tgatcgggac gtcgagccgg
     1201 atcgccccct ccatttcgat gcgcaccatc tcccggagcg cctcggcatc ggcgcggggc
     1261 acctcgaaga ccagctcgtc gtgcacctgc atgatcatcc tggcggcact cttctcctcc
     1321 tcgaggcgct tctggatccg gatcatcgcc accttgatca gatcggccgc gctgccctgg
     1381 atcggggtgt tgatcgccgt gcgctcggcg aactcgcgca cccggcggtt gtcgctggcg
     1441 atctcgggga tcatccgccg ccgcccgagc agggtggtga catagccgtt ctgacgcgcc
     1501 tcgcgcaccg tgcgcagcat gtactcctgc accccgggat agctggcgaa ataggcctgg
     1561 atgaactgct ccgcctcctc gttgctcagg ccgagccgcg acgccagacc gtaggccccc
     1621 atcccgtaca tgatgccgaa gttgacctcc ttggcccgtc ggcgctgctc cggggtgacc
     1681 tccgcctgcg gcaccttgaa gaccagggcg gcggtgcggg cgtggacatc ctcgccggcg
     1741 gtgaaagagt cgcgaagggt cctgtcgccg gagagatgag ccatgatgcg cagctcgatc
     1801 tgggagtaat cggcgtcgag gatgagatgg ccctcatccc cggggatgaa ggcgcggcgg
     1861 atgcgccgcc ccacctcggt gcggatcgga atgttctgca gattgggctc ggtcgaggag
     1921 agccggcccg tagccgccac cgtctggttg aatgaggtat gaacccggcc ggtggccgga
     1981 ttgatcagcc gcggcagggc gtcgacgtag gtcgacttga gcttggcgag ctgacggtag
     2041 tcgaggatgc gctgcggcag gggatgggca cctgccagct cctcgagcac cccgacatcg
     2101 gtcgaaaaac cggtcttggt ccggcgcgcg gaagggagct tgagttcgct gaaaaggatc
     2161 tccccgagtt gtttggggga gttgatattg aatttgcggc ccgccagctc gtagatcgat
     2221 ttttcgatca cccccagctc ctgctccagc tcccctgaca tcgcagccag ataaagctcg
     2281 tccagggcga cgccgttgcg ctccatcacc atgaggacat ggatgagcgg cacctcgacc
     2341 tcctcgagca gggggcgtag cccgaaggcg tcgagcttgg gcatcagcac ctgctgcagc
     2401 cgccaggtat aatcggcatc ctcgcaggcg tattcactca cctcctcgat tggcaccagg
     2461 tccatggtgc gctggttccg gcccgtgccg agcagctgcg aggtggggat ctttttcagc
     2521 ccgaggtgct cgagcgagac cgcgtcgaga ttgtgctgaa agcccgaggg attgagcaga
     2581 taatcggcga ccatggtatc gctggccaga ccggccacct cgacgccgtg gcgcgcgagg
     2641 acgagaagat catatttggc gttgtggccg cactttttga tcgcgggatc gctcagcagc
     2701 ggggcgagga tcttcagcac ttcaccagtg ggaaggggct gctggcccgc ggtgaggtcc
     2761 gccggcccct tgaccgggat gtaccaggcc tccccctccg cgcaggaaaa agagagtccg
     2821 accagctctg cacgcatggg atcgaccgag gtggtctcgg tgtcgagggc gaaagccccg
     2881 cagctgcgga ggcgctcagc cagctccgtc acgctcttca gatcgccgac cagctggtag
     2941 cggttcacgg cgcgggtgcg gctgctgaag cggtcgacca gggagggcag gtccatctcc
     3001 cggaaaaagg cggcggcctt ttccgccggg atcggccgca aatggagggt ttcgatgccg
     3061 atctcgaggg gtacatcgcg gtggatggtg gcgagggtac gggagaggcg cgccaggtcg
     3121 gcgttctcct gcagggcggt gcgctgtttt ttcgattcga tggcatcgac ctgttcgagc
     3181 agggcctcga gcgagccgaa acgcttgagc aaagcgaggg cgcccaccgg gccgatgccg
     3241 ggcaccccgg ggatgttgtc tgaggcatct ccgcagaggg cgagataatc gatgatctgg
     3301 gagggcggga cgcccatttt tcgagtgacc ccatcggcgt cgagccattc gatactttcg
     3361 ccggcgcgat ggggataata gatcagggta tggtcgtcga ccagctgcat gagatccttg
     3421 tccccggtga cgagcacggt ctggtagccg ctggccgccg ccgccctggc cagggtgccc
     3481 atgatgtcgt cggcctcgta gcccggcttg cggatgaagg gcagatcgag ggcgcccata
     3541 agctgttcga gtcggggcag ctgttcggcc atatcatcgg gcatgcgctg ccgctgcgcc
     3601 ttgtattccg tgtagatctc gtggcggaaa gtgggctcgg gggaatcaaa gaccacggcg
     3661 aagagatcgg gtttctcctg gtcgagcagg ttgagcagga tgcggctgaa cccgaaaatg
     3721 acgctgacct gttcgccgcg cgagttgacg agaggattgc gcgcaaaggc gaaataggta
     3781 cgataggcga gggcggcgcc atcgataaga aagagacgtt tcttgtccgc ggactgcggc
     3841 ataggggatc ccggattaga agataaagag gccaagaagg attcggggcc ggctcgggcg
     3901 agaggaaacc ttttctcgga gcattcgttt cactataagt gaatgtaaat aaattgggca
     3961 tagcatgcaa cgcaaaaatt gtgttcacat ttctgttgag aataatcccc aatttggtta
     4021 tattagtgtt taatctaatt ttgcattcaa acggaggaaa aacacgatgt cgcaggtccc
     4081 cccgcagcag ctgaacatcg aattgggcga aaaggaagcc gagggcatct attccaatct
     4141 tgcggtgatc tcccattcgc cggcggaatt tgtcatcgac tttacccgca tggtgccggg
     4201 actccccaag acccgggtct acgcccgcat catcatgacc ccgcagcacg ccaaatccct
     4261 gctcaacgcc ctcaaggaga acatcgccaa gtatgagagc cagttcggtg agatcaagac
     4321 ctattccgaa cccgtccgcg aaagcacacc catgggcttc cacgccgatc aggccggatc
     4381 tccccaggaa aaacagaatc cccgctgatg cgactgcgcg caatctgcat atgggccctc
     4441 ttcttcgccg cgggggccct cgccgccacg ccgctgaacc ggggctcgct ttccctgcag
     4501 accggcgtct ggaaacccgg caccctcgat tccgacccgg gtaaaccctt cacaccggtc
     4561 gagggcaccg gactgtgctg gggcgtgggc ctctgctccc cccatgccgc cggcttcgcc
     4621 ctcgaaatga acctctggca gtgggagcgc cagctgcagg gcagcgaggc ggagccagtt
     4681 gcgagccggc tggtcaacct cgccttcgac ctcaagtacc agctcctcgg cgccaccgcc
     4741 atccgcccct tcgtgctcta cggcggcgcc gccctctacg gccgcgagga tcccgcctcg
     4801 cccccgccgg gaatcggcag cgaacttgaa ttcgccggct acgccctcaa cttcggcgcc
     4861 ggcttcgact ttatcctttc ccgccacctc ggcctctccg cggcctatca atacctctat
     4921 gtcgatatgc gcaaggagat cggcgcaacc acccgctaca gcggtcccaa ggtcaccctc
     4981 aagctcctct acctcttctg atcccggcgg ccgtccgccg gctctgttca atcctgattc
     5041 aagggtgtct acgcctgaaa caaaccggcc ctatttttgt atctctcccg gccgtccgcg
     5101 cccctctgcc ctccactgcc cggcccctgt aaggctttaa ccttgttttt ataatattta
     5161 taacaagtcg gtatcagcaa aatattctct tccaccccaa aatttcctcc cttttaccgc
     5221 caaagggtat aaaatttgcg tgcaatcagg cgacccctca ctccgtgcgg ggcagaaacc
     5281 ggtctatctg tacaattcag cccggcgcgt ccgggcagcc tatatcggga ggatgtgcat
     5341 tgacagcaat caagaatcgg ccgctcagcg cctggctggt ggggataggc atcatcatcg
     5401 cagcatgggc cccggggctc catgcccagt ggaaaatcat gcccatcggc gattcgatca
     5461 ccgacggtgt cggcagcacc ggtgcgggct accgcagcca gctctacgac aagctgcagg
     5521 gggtgaattt cagcctggtc ggccccaacg gggcagcgcc ctataacggc catttcaccc
     5581 gcggcgccaa gattgaagag ttctatacgg gcggctacgg caccggcatc agggacatcg
     5641 cctcctccat gaacacctac cagcccgaca tcatcctcat ccatctcggc accaacaata
     5701 tgaacaatga tgatgccgcc ccctactccg agaacgccgg catctcgatg ctgccgacct
     5761 cttccggcaa gctcgcccag ctgctcgcct acgcctccca gtgggccaac ggcacgcggg
     5821 gggatttcct caagcgcatc attgtttgca agatcgtccc ccggctggtg aatggttccc
     5881 ccgaccccaa gatcgccgat ttcaacgctg aaatcgaccg catgttcttc gagaacgccc
     5941 ccggcatcgt cgtcagtaag gtcacccttg ccgacatgaa cagcgtcgtg ccgctctccg
     6001 acctcggcga cggcacccat cccaacgaca ccggctatgc caaaatggcg accgaattca
     6061 accgcatcct ccgcggcgtc atcgccggcg atcgcagcgc gccggcgagc atctcctggc
     6121 tcggggcgga cgccatcagc ggcagcaccg ccggccttaa atggtattcc accggcgacg
     6181 acggcaccaa cggacaggcc aacctctacg aaatgcgcta ttccaccttc ggcctcgatg
     6241 ccgccaattt cacccagggc attctggtct ccctgccgcg gcctcagagc gcgggcaata
     6301 tcgaaaccgc cagcgtctcg ggcctggtcc ccggcatcag ctactttttc ggcatccgcg
     6361 cctatgacga gatgaacaac cgcggcccgg tctccatcgg cccctccgtg gacatggccg
     6421 acaccgccag caccgaatac tgcgacgact ttgaggatcc cctcgccgcc gactgggacc
     6481 tcaatcccgc ctaccggatc gacgtcaccc gctacgaact ggtcaacagc gccacctcat
     6541 cgggctggca gtacctggcc gcctacaagg ccgcgcgcta tgcccccgcg gtgcgcggcg
     6601 tccgcgccgc catgcagtgg tcagagctgg ccgacgaccg tggcatcaac gccagcggcc
     6661 tcgccatgat gctcaacggc ccaagtcagc aggccagcgg ctacctggtt cgcgtccgcg
     6721 atcgcgtggt ctatctcaac gagatcgtca atggcggcgt ccccaccggc aacctcgcca
     6781 ccgcgggctt tccccccacc gcgccgga
//
LOCUS       DAOPWU010000178         6773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_11215_length_6773_cov_4.604942, whole genome shotgun sequence.
ACCESSION   DAOPWU010000178 DAOPWU010000000
VERSION     DAOPWU010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11215_length_6773_cov_4.604942"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PKM23_09665"
     CDS             complement(<1..505)
                     /locus_tag="PKM23_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HNY91780.1"
                     /translation="MKSCRLTAAMVVITIGSVAAFFAGCFVSTEPSPRRIERFTREWK
                     FHPGDLQGAEEPGFVDSGWRQLDLPHDWSIEGAFSAENPATPGGGALPGGIGWYRKHF
                     SLARADSGKIIFIDFDGVYRNSEVWINGRYLGKRPYGYSSFRYELTPWLHYGERPNTL
                     AVRVDNSR"
     gene            complement(526..3372)
                     /locus_tag="PKM23_09670"
     CDS             complement(526..3372)
                     /locus_tag="PKM23_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNY91781.1"
                     /translation="MNRLHLHLLFLAGMTFSLAATGLGYEKLADGILLQLDRKSAAGT
                     ALLKIEVCGDELIRVVASPADSFSARPSLMRVPLSGPAPSWTVRDKGALIEIRTAKLR
                     VRVQKRNGAVSFHDSRGKLILAERADGRYYTPATVMEEKTQNIRQLFHSPADEAFYGL
                     GEHQNDVMNYKGHDVDLFQLNIVDVNPFLVSSRNYGILWDNTSQTRFGDIRDFRSLAD
                     LKLFDRSGAAGGLTAEYFRSADFKELFASRTEPRIEHEFIDVNDPFPDGFKENVASVR
                     YTGQIEAPESGEFKFRLYASGYIKMWLDGVLVADNYRQNWLPWTTLPRLQMVAGKRYD
                     LKIEWIHMGGYLGLKYLPPDHATPPNSLSLWSQIGDQIDYYFVHGENFDEIIHGYRTL
                     TGQAPLMPKWALGLWQSRQRYTSQEELLSVVKEFRQREIPFDNIVLDWFYWNEDQWGS
                     HVFDASRFPDPRGMVEQLHNDLHARIMISVWPKFYVGTEHYNEFAAHPGWLYMRNVEV
                     KERDWVGPGYTCSFYDPYRAEARQLYWDQINEHLFSMGFDAWWLDATEPDIHSNLSLE
                     ETRRRIGPTGLGSSSRYLNSYTLVHSGGIYENQRKAKPDQRVFILTRSVFAGQQRYAA
                     ATWSGDIVTRWYDLKAQISAGLNFNLSGVPWWTMDIGGFSVEPRWERNVKPDDLEEWR
                     EFNARWFQFGTFVPLLRVHGEFPYREMFNIAPADHPAYQAMLAYDKLRYRLLPYLYSL
                     AAKVTFEDYTLMRALVMDFGKDARVYSIGDQYMFGPALLVNPVTAYKARARSLYLPAG
                     TGWYALNSGQYSTGGRSVIAEAPLSDIPLFVREGSIIPFGPPLQYVMEKPADPIRLYV
                     YTGRDGEFDLYEDDGTSYSYEQGQFARIPITWSEKSRILTLGARKGEFPGMLRERTFE
                     IVWVSRSRPVKLDFEADPAATVHYSGTAVSVSLK"
     gene            complement(3571..4302)
                     /locus_tag="PKM23_09675"
     CDS             complement(3571..4302)
                     /locus_tag="PKM23_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91782.1"
                     /translation="MKRSFFTLFLAAGLCSAAAAAEPRLEPARFFPAPLPLIAADEAA
                     PQAASGLDEKSRGKALMLSFLLPGLGEHYAGAGTKAKIFLGTEISLWLGYAGFLTWSD
                     WRHDEMESYAARHAGVNPAGKSDTYYANIGNYDTIQQYNEAKLRQRNLNDYYRDLDAW
                     HWEWDSAANRERFDQLRISSDKARNRGTFVLGAILANHLISAIDAVWSVNRYNDSQAS
                     RVEWNLQFGDGRIQPHLLLSMQRHF"
     gene            complement(4307..5131)
                     /locus_tag="PKM23_09680"
     CDS             complement(4307..5131)
                     /locus_tag="PKM23_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91783.1"
                     /translation="MTRKTALMVALFAALVLAGTAGATDWREFMIAAPSAQIALQDTV
                     FEEVEEEYTVDARNGGKALIFSAALPGAGQLYAGSYLKALGFIAVEAAGWYLYSDFSG
                     EGNRIEDEFEAYANQNWSEDQYWNAIASLAGLSVDNREALREWEHANFSHGLHLEKDQ
                     QYYEMIGKYNQFNYGWNDVYNTTPRPTDKIIYLKEQERSAKRLYYEGRRNASNTAFKR
                     ATGSLTLVMINHLASALDAVWTVSRHNRSIAEARLLFEPRHLDQHPYSALTLRVDW"
     gene            complement(5196..>6773)
                     /locus_tag="PKM23_09685"
     CDS             complement(5196..>6773)
                     /locus_tag="PKM23_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91784.1"
                     /translation="QARRHGRDLIILDADSGERTPLVVDDGDARDPWFSADGQRLYFA
                     WDKSGIFNIYSIRPDGGDLKLWTNTSGGAFMPSLSAEGELVWSQFQYNGYKIARLRAP
                     QSLPEEYARYAPADNADRLGLTEPATPAELAALRDHDDTRLPDIKAEPYEMTYGQISF
                     LPRAMIDYKTLKLGSYFYASDVLERYSIFGGATLNPQMDLDAFAIVEMRRFKPTLFLE
                     LYSFSRHIEREIDVIEDYPEKVNAGIRFNILEADLGARINLTEGQTLRGAFVHSRYTS
                     KIGDFFFKGIDWVSPANTYYIGNQFIATWNLNAVVPGVTSDINPRMGRKAELRYSYER
                     NRFFEDFATDNSYGTLQEVYARYNYHRVELNWHEYLPAPWASRHGLSANIHAGWIDRP
                     VDSFFNFFAGGLPGLRGYPYYSIEGRKMILGRFAYRLPLLTGVQKQFLHLNGGNLYLG
                     AFFDYGNAFDEDKIDPGDFKKTAGGSLRLNGFSFYGFPTALSFEAAYGFDRIENGGQS
                     YGREWRYYLMALFDFMD"
BASE COUNT         1456 a         1880 c         2084 g         1353 t
ORIGIN      
        1 gccggctgtt atcgacacgg acagccaggg tgttgggtct ttcgccataa tgaagccagg
       61 gggtcaattc gtaacggaaa gagctgtagc catatggccg tttgcccaga tagcggccgt
      121 tgatccagac ctcgctgttg cgatagaccc cgtcaaagtc tatgaaaatg attttaccgg
      181 agtcagcccg cgccagggag aagtgtttgc gataccagcc gatgccgccg ggaagggcgc
      241 ccccgccggg tgtggccgga ttttccgcgc tgaaagcgcc ctcgatgctc cagtcatggg
      301 gcagatcgag ctgacgccag ccgctgtcaa caaatccggg ttcctcggcg ccctggagat
      361 cgccggggtg aaatttccac tcgcgggtga agcgctcgat ccggcgcggc gatggttccg
      421 tactcacaaa gcagccggcg aaaaaggcgg caacggaccc gatggtgatc acaaccattg
      481 cggcggtcag cctgcagctt ttcatggcat tcctcttcaa gttggtcact tcagagagac
      541 gctcacagct gttcccgaat aatgcactgt cgcggcaggg tcagcctcga aatcaagctt
      601 gaccggcctg ctcctgctca cccagacgat ctcaaaagtg cgttccctga gcatccccgg
      661 gaattcgccc ttgcgggcgc cgagggtcag aatcctgctt ttttccgacc aggtgatggg
      721 aatcctggca aattggccct gctcataact gtacgaggtg ccgtcatcct cataaagatc
      781 aaactcgcca tcccgtccgg tatagacata gagccggatg ggatcggccg gcttttccat
      841 aacatactgc aggggcgggc caaaggggat gatcgagcct tcacggacaa aaagaggtat
      901 gtcgctcagg ggcgcttcgg cgatgaccga gcggccgcct gtactatact gccctgagtt
      961 gagggcatac cagccggtgc ctgcaggcag atagaggctc cgcgcacggg ctttgtaagc
     1021 cgtaaccgga ttgaccagaa gcgccgggcc gaacatatac tgatcgccga ttgagtaaac
     1081 cctggcgtcc ttgccgaaat ccatgaccag ggcgcgcatg agagtatagt cctcaaaggt
     1141 cacctttgct gcaagggagt aaagataggg cagcaaacgg tagcggagtt tgtcataggc
     1201 cagcatggcc tgataggcgg gatggtccgc cggggcgatg ttgaacatct cccggtaagg
     1261 aaactcgccg tgcacgcgga gcagaggaac gaaggtgccg aactggaacc agcgcgcgtt
     1321 gaattcccgc cattcctcaa gatcgtcggg tttgacattg cgctcccagc gcggctcgac
     1381 cgaaaaaccg ccgatatcca tggtccacca gggcacaccg gagagattga agttgaggcc
     1441 ggccgaaatc tgcgctttca ggtcgtacca gcgcgtgaca atatcgccgc tccaggttgc
     1501 cgcagcatag cgctgctgtc cggcgaagac cgaacgggtc aggatgaaaa cccgctgatc
     1561 cggtttggcc ttgcgctggt tctcataaat gccgcccgaa tggaccaggg tataactgtt
     1621 cagataacgg gaagaggagc cgagcccggt cgggccgatg cgacgccgcg tctcctcgag
     1681 cgagaggttg gagtggatat ccggctcggt ggcatcgagc caccatgcgt caaatcccat
     1741 gctgaagaga tgttcgttga tctgatccca gtagagctgg cgcgcctcgg cgcgataggg
     1801 atcgtaaaaa gagcaggtat aacccgggcc gacccaatcg cgctccttga cctccacgtt
     1861 gcgcatataa agccagccgg gatgggcggc gaactcgtta taatgctcgg taccgacgta
     1921 aaacttgggc cagaccgaaa tcatgatccg ggcgtgcaga tcgttgtgga gctgctcgac
     1981 catacccctt ggatcgggga accggctggc gtcaaagaca tggcttcccc attgatcctc
     2041 gttccagtag aaccagtcca gtacaatatt atcgaagggg atctcacgct ggcggaactc
     2101 cttgaccacc gagagaagtt cttcctggct ggtgtagcgc tggcggctct gccagaggcc
     2161 gagggcccat ttgggcatga gcggagcctg gccagtgagc gtgcgatagc cgtggataat
     2221 ctcatcgaaa ttctcgccat gcacgaagta atagtcaatc tggtcgccga tctgggacca
     2281 gagtgaaagg ctgttaggag gggtggcatg atcgggaggc agatatttga gtccgagata
     2341 gccgcccata tggatccatt cgatcttgag atcgtagcgc ttgccggcga ccatctgcag
     2401 gcgggggagg gtcgtccagg gcagccagtt ctgccggtag ttgtcagcca caagcacccc
     2461 gtccagccac atcttgatgt agccggaggc ataaagccgg aacttaaact ccccgctctc
     2521 cggagcctcg atctggccgg tgtagcgcac gctggctaca ttctccttga aaccgtcagg
     2581 aaaggggtcg ttgacatcga tgaattcatg ctcgatgcgc ggttcggtgc gtgaggcgaa
     2641 tagttcctta aaatcggcac tgcggaaata ctctgctgtg aggcctcccg cagccccgct
     2701 ccggtcaaag agtttgagat cggcgagaga ccggaaatca cggatatccc cgaaccgggt
     2761 ctgggaggta ttgtcccaaa ggatgccata attcctgctc gataccagaa aaggatttac
     2821 atcaacaata ttgagctgaa agagatcgac atcatggccc ttgtaattca tcacatcatt
     2881 ctggtgctct cccaaaccgt aaaaagcttc gtccgcgggg gaatgaaaaa gctgcctgat
     2941 gttctgggtc ttttcctcca ttactgtggc cggcgtataa taacgaccgt ccgcccgttc
     3001 tgcgaggatg agtttgcccc gggagtcatg gaaggagaca gctccgttcc gtttttgcac
     3061 acggaccctg agtttggccg tcctgatttc gatcaaggct cctttgtcac gcacggtcca
     3121 ggacggggca gggccggaga gaggaacccg catcaggctc gggcgggccg aaaagctatc
     3181 ggcaggtgaa gcgaccactc ggatgagttc atcgccgcag acctcgattt tgagcaaggc
     3241 agtaccggcc gccgatttcc tgtccagctg gaggaggata ccatccgcca gtttttcata
     3301 gccgagacct gtagcggcca gggagaaggt cattccggcc aggaacagca gatgcaaatg
     3361 caaacgattc atggctcttt cctttcatcg tggaatgcat tactgcagtt caccaattat
     3421 cctctgtttt aaaatatact aacaagaaat gataaaacca ccagaattaa tgaaactatg
     3481 gttattcgat caggaagaaa cagtccggtt aggggataat cgacggagag agacccgggc
     3541 gggagagctc ccgcccggaa aatggggagg ctagaagtgc ctttgcatag agaggagcag
     3601 atggggttgg atcctgccgt ccccgaactg gaggttccac tccacccggc tggcctgact
     3661 gtcgttgtag cggttgacgc tccagacggc atcaatggcg ctgatgagat ggttcgccag
     3721 gatagcgccg agcacaaagg tgccgcggtt gcgcgcctta tccgaggaga tacgcagctg
     3781 atcaaagcgc tcccggttcg ctgcggaatc ccactcccag tgccatgcat ccaggtcccg
     3841 ataatagtcg ttgagattgc gctggcgcag cttggcctcg ttgtactgct ggatggtatc
     3901 ataattgccg atgttggcat aataggtatc gctcttgcca gcgggattca ctccggcgtg
     3961 ccgggcggcg taactctcca tctcatcgtg gcgccagtcc gaccaggtca aaaatccggc
     4021 atagccgagc cagagactga tttccgtgcc caaaaagatc ttcgccttgg tcccggcgcc
     4081 ggcatagtgc tccccgagac cgggcagaag gaaggagagc atcagggcct tgccgcgcga
     4141 tttttcatcg agtccggagg cggcctgcgg agcagcttcg tcggccgcaa tcagcggcag
     4201 cggcgcgggg aaaaaacggg cgggttccag ccgcggttcg gctgcagcgg cagcgctgca
     4261 caatcccgca gcgagaaaca gcgtgaaaaa agatctcttc atttttctac caatccacac
     4321 gaagggttag ggccgagtag gggtgctgat ccagatggcg cggctcgaag aggagacggg
     4381 cttcggcgat gctgcggttg tgccggctga ccgtccagac ggcatcgagg gcgctggcga
     4441 gatggttgat catgaccagg gtgaggctgc ctgtggcgcg cttgaaggcg gtgttgctgg
     4501 cgttgcggcg gccctcgtag tagagccttt tggccgaacg ttcctgctcc ttgagataga
     4561 tgatcttgtc cgtgggacgc ggagtggtgt tgtagacgtc gttccagccg taattgaact
     4621 ggttatattt gccgatcatc tcgtaatact gctggtcttt ctcgaggtgc aggccgtggc
     4681 tgaaattggc gtgttcccat tcgcgcaggg cctcgcgatt gtccacggag aggcccgcca
     4741 gcgaagcgat ggcgttccag tattgatcct cgctccagtt ctgattggca taggcctcga
     4801 actcatcctc gatgcggttg ccctcacccg aaaaatcgga atagaggtac cagcccgccg
     4861 cctcgacagc gatgaagcca agggccttga ggtagctgcc ggcgtagagc tgcccggcgc
     4921 cgggcagggc ggcggagaaa atcagcgcct tgcccccatt cctggcgtcg accgtgtatt
     4981 cttcttcgac ctcctcaaag accgtatcct gcagggcgat ttgcgcgctc ggggccgcga
     5041 tcatgaattc ccgccagtcg gtcgcgccgg cggttcccgc caggacaagc gcagcaaaaa
     5101 gtgcaaccat caacgccgtc ttccttgtca tacccgtctc ccgtcgtaaa tcttccagtc
     5161 gggccagcgg gaggcctgcg cctccctgcc cgccatcagt ccatgaaatc aaagagcgcc
     5221 atcagataat agcgccactc ccggccatag ctctggccgc cgttctcgat ccggtcgaat
     5281 ccataggccg cctcgaaaga gagggcggtc ggaaaaccgt aaaaggaaaa accgttcaga
     5341 cgcagcgagc cgccagctgt ctttttaaaa tcgcccggat caatcttgtc ctcatcgaag
     5401 gcgttgccat aatcgaaaaa ggcgcccagg tagagattgc cgccgttgag atgaagaaac
     5461 tgcttttgca cccccgtcag caggggcagg cggtaggcga agcggccgag gatcatcttg
     5521 cggccctcga tactgtaata cggatacccg cgcagtccgg gcaaaccgcc ggcgaaaaaa
     5581 ttgaaaaagc tgtccaccgg gcggtcgatc cagccggcgt ggatattggc tgagaggccg
     5641 tgccggctcg cccagggggc gggcagatac tcatgccagt tcagctcgac gcggtgatag
     5701 ttatagcggg cgtaaacctc ctggagggtg ccgtagctgt tgtccgtggc aaaatcctcg
     5761 aaaaagcggt tgcgctcgta agagtagcgc agctcggcct tgcgccccat gcggggattg
     5821 atgtcggagg tgacaccggg gaccaccgca ttgagattcc aggtggcgat gaactgattg
     5881 ccgatataat aggtattggc cggcgaaacc cagtcgatcc ccttgaaaaa gaaatccccg
     5941 atcttgctgg tatagcggct gtggacgaag gcgccgcgca gggtttgccc ctcggtgagg
     6001 ttgatgcgcg caccgaggtc cgcttcgagg atattaaaac ggatgccagc gttgaccttt
     6061 tccggataat cctcgatcac atcgatctcg cgctcgatat ggcgcgagaa ggaatagagc
     6121 tcgaggaaaa gggtcggctt gaagcggcgc atctcgacga tggcgaaggc gtcgagatcc
     6181 atctgcggat taagggtggc gcccccgaag atggaatagc gttcgaggac gtcgctggcg
     6241 taaaaatagc tgccgagctt gagggtttta tagtcgatca tggcccgggg caggaaggag
     6301 atctggccgt aggtcatctc gtagggctcg gccttgatat cgggcagccg ggtgtcgtca
     6361 tggtcgcgca gggccgccag ctccgcaggg gtcgccggtt cggtcaagcc cagcctgtcg
     6421 gcattgtcgg ccggcgcata gcgggcgtat tcttccggca ggctttgagg agcgcgcagg
     6481 cgggcgatct tgtagccgtt atactggaac tgcgaccaga ccagctcacc ctcagccgag
     6541 agcgagggca tgaaagcccc gccgctggta ttggtccaga gtttgagatc cccgccgtcg
     6601 ggccggatac tgtagatgtt aaagattccg ctcttgtccc aggcgaaata gaggcgctgg
     6661 ccgtcggcac tgaaccaggg atcgcgggcg tcgccgtcat cgacgacaag aggggtgcgc
     6721 tcaccgctgt cggcgtcgag gatgataagg tcgcggccgt gacggcgcgc ttg
//
LOCUS       DAOPWU010000179         6759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_11244_length_6759_cov_4.405131, whole genome shotgun sequence.
ACCESSION   DAOPWU010000179 DAOPWU010000000
VERSION     DAOPWU010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11244_length_6759_cov_4.405131"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKM23_09690"
     CDS             complement(<1..463)
                     /locus_tag="PKM23_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNY91785.1"
                     /translation="MDVFKLESPFTLQGDQPQAVEQLVEGLRRGDGYQTLLGVTGSGK
                     TFTMAHVIARINRPTLIISHNKTLAAQLYGEFRGFFPNNAVEYFISYYDYYQPEAYLP
                     HTDTYIEKDTSINDEIDRLRLRATSALLSRRDVIIVASVSCIYGLGSPEDWL"
     gene            643..2859
                     /locus_tag="PKM23_09695"
     CDS             643..2859
                     /locus_tag="PKM23_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNY91786.1"
                     /translation="MRNGLGLPGTLLLAILISFHFVIPASAQNDAETRALWVTRWDYS
                     SPADIGRIMENAASARFNTILFQVRGDGTVLYRSKIELWSSELRNGDPGWDPLQVAVD
                     YAHAHRLKLHAWINVYPGWMGIKPPRIGNQLYNAHPDWFMVDRDGQRQPLSSHYVWLS
                     PTHPEVTPYLLSLCEEIYSNYAVDGLHLDYIRYPGPAYSYDQPSLDAYRQENGGTPRG
                     DEKAWSQWRREAISRFLGRLHSAMRLHRPELMLSAAVLGNYQTGRRLYLQDSHEWLAR
                     GIVDAIFPMIYTRDDTLFRRQLLEHRYNDHNRHVYPGLYTGSVRHLSAQIDIAREAGC
                     KGLAIFSYELLFPNHSPDPGFAEVLARSWGSEAPVALQPWKMYAGDAQGPVVEQAYTI
                     PDRLFAHSKFRIAARITDPSGVFDDNTGSKGKGIYLLYDRSWPPNPANEVQMSKLKNY
                     KDWYITDKAIKADYAGLDFRFRVHAWDNHQESAGHFKRNFGYSDVWSLSILAKYQTFI
                     AKGTFGPVIESPCAITADGRGQLWVGSLNTPALTILDSRGRPASFSPSSTHSTTISGA
                     TPSGSSAPWLLRPPTSSASSPRKSPACSTASRLKTASPCRALPLISRPAGLTATAKGG
                     FSCSRRTRRAGTSSARSESRSAAAPSASITPAPISPCSGTAARSISPTRPAAGCSAGT
                     GQLRAIWRATGGSRISRRRMPATARSGAIPATRSMCLIPSAGSSPSLTVRAGCLPI"
     gene            complement(3073..3960)
                     /locus_tag="PKM23_09700"
     CDS             complement(3073..3960)
                     /locus_tag="PKM23_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNY91787.1"
                     /translation="MQKEDEILAGGGDPPVFRRAIVAVALSPSIQAVLNQSQRILEIL
                     GARPVLLHVGQDTPAARAKLQKAIAASRFAELAPELIIQPGQPAEAIAAAACQQRADL
                     IITGAARKESLLRHFIGSVAPHLARQTPCSLLMMAGASDDRSSFTRIHCEVEYDRPAH
                     FAIEAAMGIARRMHSRELVLTHSFQVEEWEGKGKTAPVNGEIRRVYHHQDTRLKRFLT
                     RYPANGLRLRTQCIHELSLASTLDFARDIAADLMVIPGPRRQTGIWDRLFGENLEQLF
                     KSLSGAILLTRRTRYRPRG"
     gene            complement(4000..4683)
                     /locus_tag="PKM23_09705"
     CDS             complement(4000..4683)
                     /locus_tag="PKM23_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91788.1"
                     /translation="MKRGWIVVAAGLAVAALRCSDPAKPEASVPEPPAMAAVSGPASV
                     NAPPQIAQKAGEILTYFNFVQTTLGDLSGSDPAVNSNIYLWEIPTGSGTIVKKIKAVR
                     RSDGSVDWTVVLNGQLADSTRYNDRQLFIGKAAEDNRSQSWTFYDLLTGAITHKISWN
                     RDKNGALQLDDTFAALNAVWHLVNARDGSGSYSFTLGGVKQFTATWRGDGSGSFIDHT
                     SSGAAESWE"
     gene            4827..>6759
                     /locus_tag="PKM23_09710"
     CDS             4827..>6759
                     /locus_tag="PKM23_09710"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNY91789.1"
                     /translation="MTSVKPVAMLLGGIAMLAQIVLLRAFMAIFWGNELLVGLILAVW
                     MATSGLGSWLGNRGVSSRPGPLLPLTLLLQAAALTAAIAALLLLPGARTILHVPAAGY
                     LTTNHLFLLSLLTIVPVAFPLGCSFTLLARAAAPASANPAALVYVWDAAGCVAGSLLF
                     TFILVRWTSPVQALLAAALLLLLMAGWRLGWRGWHPPAGLLLVMLFLHAPGIEQNRAA
                     AYWRSFAPGMDLRTRATSRHGELAVVDWHGATSLFSNGALQATLPNPVDSQADAALLM
                     NQSLPSPGEILLIGDGLGGLAPELARYPEASVTCIELDEEAGRLALNALPDSLAAAWR
                     NPRLTLRFTDGRHFLQRSDRRYALIIVDAGRPSGALANRYYTEEFFALAKSRLLPGGV
                     LALLNVPSGENYLGPELLRLNSSIYSGLRRFFTEIIVIPGGEALFLAGVHSRITADPD
                     ELARRLEEKKLALNCFYPSMFAFMLPAERISELENRLASAPPRRNLDFQPVVYFIDLL
                     LWQKMIQGGGTLLLAIERLGYARLLLIWCTILTLLLLPALLRTAAPHGRSHEAAVRRA
                     LLFAAFSVGMAATAFDVLFIMAMQNIFGNLYAAIGLALAAFMAGMAGGGWLALRRERR
                     GFLLPLLLLLLIALSALVLT"
BASE COUNT         1173 a         2278 c         1960 g         1348 t
ORIGIN      
        1 ccagccagtc ctcgggcgag cccaggccgt agatgcagga gaccgaggcg acaatgatca
       61 catcgcggcg tgacaggagc gccgaggtgg cgcgcaggcg cagccggtcg atctcgtcat
      121 tgatcgaggt atccttttcg atataggtat cggtatgggg gagataggcc tcgggctgat
      181 aatagtcata atagctgatg aaatactcga ccgcattgtt cggaaagaag ccgcggaatt
      241 cgccgtagag ctgggccgcc agggtcttgt tgtgcgagat gatcagggtc ggacggttga
      301 tgcgggcgat gacgtgggcc atggtgaagg tcttgccgct gccagtcacc ccgagcaggg
      361 tctggtagcc atccccgcgc ctcagcccct cgacaagctg ttcgaccgcc tggggctggt
      421 cgccctgaag ggtgaagggg gattcgagtt tgaagacatc catttgctga gcctcaggat
      481 aagcggattc tacgttccaa gcaattaatg tactatgaat gcgggaaata gtcaagctgg
      541 atttgtgctc agaaagcctt gaaaaaccgc aataaactgt ttgaaagttt ggctgtattt
      601 tgctaaatta gggccaatca tttgaccaga tcgggagttt gcatgcgcaa cgggttgggt
      661 cttccgggca ctcttcttct cgccatactc atctcattcc attttgtcat ccccgccagc
      721 gcccagaatg atgcggaaac ccgcgccctc tgggtgacgc ggtgggatta cagttccccg
      781 gcggacatcg gccgcatcat ggaaaatgcc gcctccgccc gcttcaacac catcctcttt
      841 caggtgcgcg gcgacggcac cgtcctttat cgctcgaaaa tcgaactgtg gtcctcggaa
      901 ctgcgcaacg gcgatcccgg ctgggatccc ctccaggtgg cggtagacta tgcccatgcc
      961 caccggctca agctgcatgc ctggatcaat gtctatcccg gctggatggg cattaaaccc
     1021 ccgcgcatcg gcaaccagct ctacaacgcc catcccgact ggttcatggt cgatcgcgac
     1081 ggacagcgcc agcccctctc ctcgcattac gtctggctct cccccaccca tccggaggtc
     1141 accccctatc tgctcagcct ctgtgaggag atatattcca actatgccgt cgacggcctc
     1201 cacctcgact atattcgcta tccgggtccg gcttactcct acgaccagcc ctcgcttgac
     1261 gcctaccgtc aggagaatgg ggggacgccg cggggggatg agaaggcctg gagccagtgg
     1321 cggcgcgagg cgatcagccg cttcctgggc aggctgcact ccgccatgcg cctgcatcga
     1381 cccgagctga tgctctccgc ggcggtgctc ggcaattatc agaccggccg ccggctctat
     1441 cttcaggaca gccatgaatg gctcgcccgc ggcatcgtcg acgccatctt cccgatgatc
     1501 tacacccgcg acgacaccct cttccgccgc cagctgctcg aacatcgcta caacgaccat
     1561 aaccgccacg tctatccggg cctctacact ggcagcgtcc gccacctctc cgcccagatc
     1621 gacatcgcgc gcgaggccgg ttgcaagggg ctggccattt tttcctacga gctgcttttc
     1681 ccgaaccact ctcccgatcc cggcttcgcc gaggtgctgg cccggagctg gggcagcgag
     1741 gctcccgtcg ccctccagcc ctggaagatg tacgcgggtg acgcccaggg cccggtcgtc
     1801 gagcaggctt ataccatccc ggaccgcctc ttcgcccatt ccaaattcag gatcgcggcc
     1861 aggatcaccg acccctcggg ggtcttcgac gacaacactg gctccaaggg caagggcatc
     1921 tatctgctct acgaccgcag ctggccgccc aatccggcca atgaagtgca gatgagcaaa
     1981 ctcaagaatt acaaggactg gtacatcacc gacaaggcga tcaaggctga ctacgccggc
     2041 cttgacttcc gcttccgcgt tcatgcctgg gacaatcatc aggagtcggc cggccacttc
     2101 aagcgcaatt tcggctacag cgacgtctgg tctctctcca tcctggccaa ataccagacc
     2161 ttcatcgcca agggaacctt cggaccggtc atcgaatccc cctgcgccat caccgccgac
     2221 ggccggggac agctctgggt gggctcgctc aacaccccgg ccctgaccat cctcgacagc
     2281 cgcggccggc cggcctcctt ctccccatcc agcacgcact cgacaacgat ctcaggagcc
     2341 accccctcgg gatcatcagc gccctggctt ttgcgccccc caacctcatc tgcgtcctca
     2401 ccgaggaaga gccccgcatg ctctaccgct tccaggctga aaacggcatc cccatgccgg
     2461 gcattgccct tgatttcccg gccagcggga ttgactgcga ccgcgaaggg cggtttttcc
     2521 tgctcgagga gaactcgacg cgctggcacg tcctcagccc gctcggaatc gagatcagcg
     2581 gcagcccctt cggcatcgat cacaccggca ccgatatcgc cgtgctcagg gacggcagcc
     2641 aggtctatat ctccgaccag accagcggcg gggtgcagtg ctggaacggg gcaattgagg
     2701 gctatctggc gcgctactgg cgggtcaagg atctcgaggc ggcggatgcc ggcgacggca
     2761 aggtcgggcg cgattccagc gacgcggtct atgtgcctca tccccagcgc ggggtcatca
     2821 ccatctttaa ccgttcgggc cggctgcttg cccatttgag cggcggaaat ccccccctca
     2881 atgcccccga agccctggcg atctcgccgt caaacgattc ccttttcgtg cttgaatcgg
     2941 ccggcagggg ccccggaacc ctctccctct gggtgcgcaa aaaatagcag gtcactcgcc
     3001 cccggcgccg tcattaaaag taaaagcccg cctccatcaa agaaacgggc ttttttgtgg
     3061 gccaaagaaa aatcaaccgc gcgggcggta gcgtgttctg cgcgtgagca ggatggcgcc
     3121 ggagaggctc ttgaagagct gttcgagatt ctcaccgaag agacggtccc agatgccggt
     3181 ctgccgccgg gggcccggaa tgaccatgag atcggcggcg atgtcgcggg cgaagtccag
     3241 ggtgctggcg agggagagtt cgtgtatgca ctgggtgcgc aagcgcaggc cattggcagg
     3301 atagcgggtg agaaaacgct tcaatcgagt gtcctgatgg tggtaaaccc gccggatctc
     3361 gccgttcaca ggtgcggttt tacctttgcc ctcccactcc tccacctgga aggagtgggt
     3421 caaaaccagc tcacggctgt gcatgcgccg ggcgatgccc atggccgcct cgatggcgaa
     3481 gtgggcgggg cggtcatact cgacctcgca gtggatgcgg gtgaaggaac tccgatcatc
     3541 cgatgctccg gccatcatca gcagggagca gggggtttga cgggcgagat gaggggcgac
     3601 cgagccgatg aaatgacgga gcaggctctc ttttctggcc gcgccggtga tgatcagatc
     3661 ggcgcgctgt tgacaggccg ccgcggcgat ggcctcggcc ggctgccccg gctgaatgat
     3721 gagttcgggc gccagttcgg caaagcggga ggcggcgatg gccttttgaa gttttgcccg
     3781 cgctgccggg gtatcctggc cgacgtgcag cagaaccggc ctggccccca ggatctcgag
     3841 gatgcgctgg gattgattga gaaccgcctg aatactgggc gaaagggcga ccgccactat
     3901 cgcccgtcga aaaacgggag ggtcgccccc tcccgccaat atctcatcct ctttttgcat
     3961 ctctgattcc gcctgatgcc tccgccgggg aggcgccgtt cactcccagc tctccgccgc
     4021 gcccgaggat gtgtgatcga taaaacttcc cgaaccgtcc ccgcgccagg tggcggtgaa
     4081 ctgcttgacg ccccccaggg tgaagctgta gctgcccgag ccgtccctgg cgttgaccag
     4141 atgccagacc gcattgaggg cagcgaaagt gtcatccagc tgcaaggcgc cgtttttgtc
     4201 ccggttccac gaaatcttgt gggtgatggc gcctgtgagc agatcgtaaa aggtccagga
     4261 ttgcgagcga ttatcctcgg ctgccttgcc gataaaaagc tggcggtcgt tgtagcgcgt
     4321 gctgtccgcg agctgcccgt tcaaaaccac cgtccagtcg acggaaccgt ccgaacgcct
     4381 gacggctttg atctttttca cgatcgtgcc gctgccggtg gggatttccc acaggtagat
     4441 attgctgttg accgccggat ctgatccgct caggtcgccc agggtcgtct gcacaaaatt
     4501 gaagtaggtg aggatctcgc ccgccttttg cgcgatctgc ggcggtgcgt tcaccgatgc
     4561 cggcccgctg acggcggcca tcgccggcgg ctcgggcacg gatgcctccg gcttggctgg
     4621 atccgaacag cgcagtgctg ccactgccaa tcccgccgcc accactatcc atcctctctt
     4681 catccctgcc tcccgtttta ctgcttagtg taaatttcaa atttaacaaa gttgtggaaa
     4741 atagcaagtc aagttttcgt ttttctttgt ttatgcgcct atttttcata aattggaaac
     4801 agtgcggcaa actcgagagg gaaataatga cttccgtcaa gcccgtcgcc atgctgctgg
     4861 gggggatcgc catgctggcg cagatcgtgc tgctgcgcgc cttcatggcc atcttctggg
     4921 gcaatgagct gctggtggga ctcatcctcg cggtctggat ggccacctcc ggactgggaa
     4981 gctggctggg caaccgggga gtctcctccc gtcccggccc actcctgccg ctcacccttc
     5041 tgctgcaggc tgccgccctg accgctgcga tcgccgctct tcttctcctg cccggagccc
     5101 gcaccatact ccatgtgccc gcggccggct acctgacgac gaatcacctc tttttactct
     5161 ccctgctcac gatagttccc gtcgcctttc ccctcggctg ttccttcacc ctgctggcgc
     5221 gcgccgccgc acccgcttcc gccaatccgg cggctctggt ctatgtctgg gacgccgccg
     5281 gctgcgtcgc cggcagcctc ctcttcacct tcattctggt gcgctggacc tcgcccgtgc
     5341 aggccctgct ggccgccgcc ctgctgctgc tccttatggc ggggtggcgt ctgggctggc
     5401 gaggatggca tccacccgct ggactcctcc tggtgatgct cttcctgcat gcgcccggca
     5461 tcgagcaaaa cagggccgcc gcctactggc gctccttcgc ccccggcatg gatctgcgca
     5521 cgcgcgccac ctccagacac ggggagctgg cggtggtcga ctggcatggc gccaccagcc
     5581 tcttcagcaa cggcgccctg caggccaccc tcccgaatcc ggtcgacagc caggccgatg
     5641 cagctctgct catgaaccag tccctgccca gccccggcga gatcctcctc atcggcgacg
     5701 gcctgggcgg cctcgccccc gaactggcgc gctatcccga ggcctccgtc acctgcatcg
     5761 aactggacga ggaggccggc aggctcgcgc tgaacgcgct ccccgactca ctggccgcgg
     5821 cctggcggaa cccccgcctg accctgcgtt tcaccgatgg ccgccatttt ctgcagcgca
     5881 gcgatcgccg ctacgccctc atcatcgttg acgccggccg gccctccggc gccctcgcca
     5941 accgctacta cactgaggag ttcttcgccc tcgccaaaag ccggctcttg cccggtgggg
     6001 tgctggcctt gctcaatgta ccctcgggcg agaactatct cggcccggag ctgctgcgcc
     6061 tcaacagctc catctatagc gggctgcggc gattttttac agagatcatc gtgatccccg
     6121 gcggagaggc cctcttcctg gccggggtgc acagccgcat tacagccgac ccggatgagc
     6181 tggcgcgccg tcttgaagag aaaaaactcg ccctcaattg tttctatccc tcgatgttcg
     6241 cctttatgct gcctgctgag cgtatctccg aactcgagaa ccggctcgcc agcgcgcccc
     6301 cccggcgcaa tctcgatttc cagccggtgg tctacttcat cgacctgctc ctctggcaaa
     6361 agatgatcca gggaggagga accctcctgc tcgccatcga gagactgggc tatgccagac
     6421 tgctgctgat ctggtgcacc atcctcaccc tcctgcttct gcctgccctc ctgcggacgg
     6481 cggctccaca cggccgcagc catgaggccg ctgtgcgccg ggccctcctc ttcgccgcct
     6541 tttccgtcgg catggccgcc accgctttcg atgtgctctt catcatggcc atgcagaaca
     6601 tcttcggcaa cctctacgcc gccatcggac ttgcccttgc cgcctttatg gccggtatgg
     6661 ccggcggggg ctggctggct ctgcgcaggg aacggagggg atttcttctc cccctcctcc
     6721 tgctccttct gattgccctg agcgcccttg tcctcaccc
//
LOCUS       DAOPWU010000180         6755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_11254_length_6755_cov_5.652388, whole genome shotgun sequence.
ACCESSION   DAOPWU010000180 DAOPWU010000000
VERSION     DAOPWU010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11254_length_6755_cov_5.652388"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PKM23_09715"
     CDS             <1..745
                     /locus_tag="PKM23_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNY91790.1"
                     /translation="LTAPRAEKEAPLTQAAALQAIAGHIGRLQQEGRLNEAALCGSAW
                     ATVEDNYMLRRLFADGLGIRTVLLNAPAPEGEDVVYKSGFTVRADKSPNRRGAELVLG
                     SSRELWQGLREGKIRTLFLLGGGAGLQLTGEQKELLDGLELLVVWDVAAGDLVRSADL
                     ALPMTSFAEEEGTFVNVQGRLQRMHAALQPPAGIKPAWRSLADLAALLQQPVTAVTAA
                     DIFNDLAGRLPAFAGLSYFKLAEVGVQLS"
     gene            755..1810
                     /gene="nuoH"
                     /locus_tag="PKM23_09720"
     CDS             755..1810
                     /gene="nuoH"
                     /locus_tag="PKM23_09720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNY91791.1"
                     /translation="MNELGFAWIDIGILLLKILVVIVVNLVILMIMEWAERRVSAFIQ
                     DRLGPNRVGPAGLFQAVADGIKFFFKEEVVPTGADKWLYTFAPLLVLVPALMTFAAIP
                     FGTTLELFGRAEPLQIVDLNIGFLYIFALVSLGVYGVVLGGWASNSKYPFLGGLRASA
                     QMISYELALGMSVIGIVMITGSLRLSTIVEYQNGLLLGFLPRWNVFLQPLAFITFLVA
                     AFAETNRLPFDLAEAEPELVGGYHTEYSSMKFAMFFMGEYIALITTSALLTTLFFGGW
                     DFPWVDEKALGIWGVLLSIAAFALKTGFFLFFFLWVRWTIPRFRFDQLMRIGWKVLIP
                     LALLNIVLTGAGLLFVH"
     gene            1916..2425
                     /locus_tag="PKM23_09725"
     CDS             1916..2425
                     /locus_tag="PKM23_09725"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNY91792.1"
                     /translation="MGVKNVGYRPARWDSSYFPGILKGMWITIKHFFSKKYTVSYPEE
                     KWPVPPGHRGAIRLNKDEQGRIKCVACEMCSTACPAKCITMKAGVAPWPDRDKYPVEF
                     RIDMLRCILCGMCQEACPEEAIELTEIYDFSAHSREALIWDKERLLRNWDITADGKYF
                     ERSKTETGM"
     gene            2498..3010
                     /locus_tag="PKM23_09730"
     CDS             2498..3010
                     /locus_tag="PKM23_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNY91793.1"
                     /translation="MIPILFFFFAGLAIASALLMVTHKNPVYCAIFLIITFFALAGLY
                     FLLGAQFLSVVQVIVYAGAIMVLFVFVIMLLNLSEEVRLPAGRRLQSGFGIVFAAILG
                     VQLLVIAAAGTRVFGRLSGDTPVSGTVERLGAALFSTYLYPFEIASVLLLTGIVGAII
                     LGSKKLPKEQ"
     gene            3024..3332
                     /gene="nuoK"
                     /locus_tag="PKM23_09735"
     CDS             3024..3332
                     /gene="nuoK"
                     /locus_tag="PKM23_09735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNY91794.1"
                     /translation="MNAIPLNFFLLVAAVLFAIGVAGVLVRRNALVILMSIELMLNAV
                     NLTFITFAAKWQALDGQLFVFFIMAVAAAEVAVGLAVIISVFRLRGTVHVDEINLLKG
                     "
     gene            3420..5363
                     /gene="nuoL"
                     /locus_tag="PKM23_09740"
     CDS             3420..5363
                     /gene="nuoL"
                     /locus_tag="PKM23_09740"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNY91795.1"
                     /translation="MFDLVWLIPLFPLLGLIINALTGWKSAGVRPGVIASAAVGLSFA
                     AAVALFFELLRLPAADRLVEVPLFKWIAAGALSTEIAFQIDPLSMVMILVVTGVSFII
                     HIYAIGYMHGDRGFNRFFIFLNLFVFAMLILVTANNFLMMFIGWEGVGLCSYLLIGFW
                     YQDDYNAYAGRKAFVVNRIGDFGFLLAIFLIYATFRTLDFTEVFSQAAAMPMGAGVLT
                     AIALLLFVGAAGKSAQLPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMVARANVIYT
                     LAPTAAAVVAIVGALTALYAATIGLTQFDIKRVLAYSTISQLGYMFIGVGVGAFAAGI
                     FHLMTHAFFKALLFMAAGSVMHAMSNQTDMRIMGGLRTKMPRTFWTMLIGTLAIAGIP
                     GFSGFFSKDEILWQAWSGAHGAPWLWLIGAVAAALTTFYMFRLIFMTFFGTLRADDKT
                     AHHLHESPGVMTWPLIILAVLSLIGGYVGIPAVLGGSNHFEHFLAPVFAASAELRGVA
                     AHGGAEGHATGHSLELLLMGIVLVLVLFSLWLAWRYYGRKSDLPARTAARLGGAYRLV
                     FNKYYVDEFYDAVIARPLHRISESALWRFIDVRIIDGSINGTAALIGAGARALSALQT
                     GVVQNYALAFVFGVIALLVYLFV"
     gene            5398..>6755
                     /locus_tag="PKM23_09745"
     CDS             5398..>6755
                     /locus_tag="PKM23_09745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNY91796.1"
                     /translation="MSDLSTMPILTILTFLPLAGALVLLFVPRQREALIRWFTLVLTL
                     AVFLLSLPLFTAFDGANPGFQFEENRSWIPSAGIGYHLGVDGISLLMVMLTAFLTPLV
                     ILSSWRSVTKKVKEYMIMFLVLETAMLGVFLSLDMVLFYLFWEGMLIPMYFIIGVWGG
                     ERRLYAAIKFFLFTMFGGVLMLVAILVLYFMHGGQSGLYTFDYLALSQMLIASKSQVW
                     LFFAFGIAFAIKVPMFPFHTWLPDAHVEAPTGGSVILAGILLKMGTYGFLRFCIPLFP
                     TAAQQYGWLIMLLAVIGIIYGALVSWVQPDLKKLVAFSSVSHLGFVMLGLFALTLQSV
                     QGGLIQMVNHGLSTGALFLLVGMIYERRHTRLIADFGGLARQMPVFVTFFLIITLSSI
                     GLPGLNGFVGEFMILLGTFEVNRLLAVLATTGVILSAVYMLTMFQRVNFGKLDKPENQ
                     EL"
BASE COUNT         1050 a         2213 c         1943 g         1549 t
ORIGIN      
        1 cctgaccgcg ccgcgcgctg aaaaagaggc ccccctcaca caggcagcag ccctgcaggc
       61 catcgccggc cacatcggca ggctgcagca ggaggggcgg ctgaatgagg ccgctctgtg
      121 cggatcggcc tgggcgacgg tcgaggacaa ctatatgctg cgccgtctct ttgccgacgg
      181 cctcggcatc cgcaccgttc tgctcaacgc ccctgccccg gagggagagg atgttgtcta
      241 caagagcggc ttcaccgttc gcgccgacaa gagccccaac cgccgcggcg ccgagctggt
      301 gctgggcagc agccgggagc tctggcaggg gctgcgagaa ggcaaaatcc ggacgctctt
      361 tctgctcggc ggcggagccg gactgcagct gaccggggag caaaaggagc tcctggacgg
      421 cctcgaactg ctggtggtct gggatgtcgc cgccggcgat ctggtgcggt cggccgatct
      481 ggccctgccc atgacctctt tcgccgagga ggagggaacc ttcgtcaacg ttcagggccg
      541 actgcagcgg atgcacgccg ccttgcagcc gcccgccggg atcaagccgg cctggcgcag
      601 cctggccgac ctggccgcgc tgctgcagca gccggtgacg gcggtcaccg cggccgacat
      661 tttcaatgac cttgcaggcc gcctcccggc cttcgccggg ctttcgtatt tcaaacttgc
      721 tgaagtgggt gttcaactga gctaaggaac cggtatgaac gaattgggat tcgcctggat
      781 cgacataggc atccttttgc tgaagatcct cgtcgtcatc gtcgtcaacc tggtcatcct
      841 catgatcatg gaatgggccg aacgccgtgt cagcgccttc atccaggacc gtctcggccc
      901 caaccgtgtc ggaccggcgg gacttttcca ggcggtggct gatggcatca agttcttctt
      961 caaggaggag gtcgtcccga ccggtgcgga caaatggctc tataccttcg cccccctgct
     1021 ggtgctggtg ccggccctga tgaccttcgc cgccattcct ttcggcacca ctcttgaact
     1081 cttcgggcgc gccgaacccc tgcagatcgt cgacctcaac atcggctttc tctacatctt
     1141 cgccctggtt tcgctcgggg tttacggcgt ggtcctcggg ggctgggcgt cgaacagcaa
     1201 atatcccttt ctgggcggcc tgcgcgcctc ggcacagatg atcagctatg agcttgccct
     1261 cggcatgtcg gtcatcggca tcgtcatgat caccggttcg ctgcgcctga gcactatcgt
     1321 cgaataccag aacggcctgc tcctcggctt tttgccgcgc tggaacgtct ttctccagcc
     1381 cctcgccttc atcacctttc tggtcgccgc cttcgccgag accaaccggc tgcccttcga
     1441 cctcgccgag gccgaacccg aactggtcgg cggttatcac accgaataca gctcgatgaa
     1501 gttcgcgatg tttttcatgg gcgagtatat cgccctcatc accacctcgg ccctgctcac
     1561 caccctcttt ttcgggggct gggatttccc ctgggtcgac gaaaaggcgc tgggcatctg
     1621 gggcgtgctg ctctcgattg ccgcctttgc cctcaagacc ggctttttcc ttttcttctt
     1681 cctctgggtg cgctggacca tcccgagatt ccggttcgac cagctcatgc gcatcggctg
     1741 gaaggtgttg atccccctgg ccctgctcaa catcgtgctc accggcgccg ggctgctgtt
     1801 cgtacactag gaggggatgc aggcggaaaa tcgtcaggcc tccggaaggg ctgtgaggag
     1861 ccgctggctc aaccgcccgc aagtcatgct gtcataatgc aatccaggga gttttatggg
     1921 tgtcaaaaac gtcggctatc gccctgcccg ctgggattct tcctacttcc cgggtatcct
     1981 caagggcatg tggatcacga taaagcattt tttctcgaaa aagtataccg tctcctatcc
     2041 cgaggagaag tggccggtgc cgccgggcca ccgcggcgcc atccgcctca acaaggacga
     2101 acaggggcgg atcaagtgcg tggcctgtga aatgtgctcg accgcctgtc ccgccaaatg
     2161 catcaccatg aaggcggggg tggccccctg gccggaccgc gacaaatacc cggtcgagtt
     2221 caggatcgac atgctgcgct gcatcctctg cggaatgtgc caggaggcct gcccggagga
     2281 ggcgatcgag ctgaccgaga tctatgattt ttctgcccac agccgcgaag cgctgatttg
     2341 ggacaaggag cgtctgctca gaaactggga cataacggcg gacggcaagt atttcgaacg
     2401 cagcaagacg gagacgggga tgtagccccg tgaccccgcc cggcatgacc ggctgcagcc
     2461 ggcaatcggg ctaaaacctg taacccggga acagagcatg attccgattc ttttcttttt
     2521 cttcgccgga ctggcgatcg catcagccct tctgatggtg acccacaaga atccggtcta
     2581 ctgcgccatt tttctcatta tcaccttctt cgccctggcc gggctctatt tccttctcgg
     2641 cgcccagttc ctcagcgtcg tccaggtgat cgtctacgcc ggcgccatca tggtcctctt
     2701 tgtctttgtc atcatgctgc tcaacctcag cgaagaggtg cggctgcccg ccggacgccg
     2761 cttgcagtcc ggcttcggca tcgttttcgc cgccatcctg ggtgtgcagc tgctggtgat
     2821 tgcagccgcc ggcacccggg tcttcggccg gctgagcggc gacacgccgg tctccggcac
     2881 ggttgagaga ctgggcgcgg cccttttcag cacctatctc tatccctttg aaatcgcctc
     2941 ggtgctgctc ctcaccggca tcgtcggcgc catcatcctc ggcagtaaaa agctgcccaa
     3001 agagcaatga ggatccgatc gctatgaacg ccatccccct gaactttttt ctgctcgttg
     3061 cggccgttct cttcgccatc ggcgtggcgg gcgtgctggt tcgccgcaac gccctggtca
     3121 ttttgatgag catcgagctg atgctcaacg cggtcaatct gaccttcatc accttcgccg
     3181 ccaaatggca ggcgctggat ggccagctgt tcgtcttttt tatcatggcc gtggccgcgg
     3241 ccgaggtggc ggtcggcctg gcggtcatca tctcggtctt tcgtctgcgc ggcacggtgc
     3301 acgtcgacga gatcaatctg ctcaagggtt gatccgatcc ggcctcccgc cgtgcaatgg
     3361 cgatggaggc gaaggagcgg agtggacccg gacaccttgc gagaactaca ggagcctaca
     3421 tgtttgatct ggtttggctg atacctctat tccccttgct ggggttgatc atcaatgccc
     3481 tgaccggctg gaagagtgcc ggcgtcaggc cgggcgtcat cgccagcgcg gctgtggggc
     3541 tctcctttgc tgcggctgtg gcccttttct ttgagctgtt gaggctgccc gccgccgatc
     3601 ggctggtgga ggtgcccctc ttcaagtgga tcgccgccgg ggctctcagc accgaaatcg
     3661 cctttcagat cgatcccctt tcgatggtga tgatcctggt cgtcaccggg gtctccttca
     3721 tcattcatat ctacgccatt ggttacatgc acggcgaccg cggctttaac cgtttcttca
     3781 tctttctcaa ccttttcgtt tttgcgatgc tgatcctggt gacagcgaat aactttttga
     3841 tgatgttcat cggctgggag ggggtcggcc tctgctccta tctcctgatc ggcttttggt
     3901 accaggacga ttataacgcc tatgccggcc gcaaggcctt tgtcgttaac cgcatcgggg
     3961 atttcggctt tctcctggcg atatttctca tctacgccac ctttcgtacc cttgatttca
     4021 ccgaggtctt tagccaggcc gcggccatgc cgatgggtgc cggggtgctc acggccatcg
     4081 ccctgctcct cttcgtcggc gccgccggca agtcagccca gctgcccctt tatgtctggc
     4141 tacccgacgc catggccggt ccgaccccgg tcagcgccct gatccatgcc gccaccatgg
     4201 tcaccgccgg ggtctatatg gtcgcccgcg ccaatgtcat ctacaccctc gccccgacgg
     4261 ctgcggcggt cgtcgccatt gtcggcgccc tcaccgccct ctatgcggcg actattggcc
     4321 tgacccagtt cgacatcaag cgggtgcttg cctattcgac catcagccag ctcggctaca
     4381 tgttcatcgg agtcggggtc ggcgccttcg ccgccggcat ctttcacctc atgacccatg
     4441 cctttttcaa ggccctgctc ttcatggcgg ccggcagtgt catgcacgcc atgtccaacc
     4501 agaccgatat gcgcatcatg ggcggcctca ggacaaagat gccgcgcacc ttctggacca
     4561 tgctcatcgg caccctggcg atcgccggca tccccggctt ttccggtttt ttcagcaagg
     4621 atgagatcct ttggcaggcc tggtccggcg cccacggagc gccctggctc tggctcatcg
     4681 gggccgtcgc cgccgccctg accacctttt acatgttccg cctcatcttt atgacctttt
     4741 tcggcaccct gcgcgccgat gacaagaccg ctcatcatct ccatgaatcc cccggtgtga
     4801 tgacctggcc cctgatcatt ctcgcagtgc tctcgctcat cggcggctat gtcggcatcc
     4861 cggcggtgct cggcggcagc aaccactttg agcatttcct cgccccggtt ttcgccgcca
     4921 gtgcagagct gcggggtgtt gcggcccacg gcggggcgga gggccatgcg accggccaca
     4981 gtcttgagct gctgctcatg ggcatcgtcc ttgtgctcgt cctcttcagc ctctggctgg
     5041 cctggcgcta ctacgggcgc aagagcgacc tgcctgcgcg cacggccgcc cggctcggcg
     5101 gggcctaccg gctggtcttc aacaagtatt atgtcgacga gttctacgat gcggtgatcg
     5161 cccgtcccct gcaccggatc tccgaatcgg cgctctggcg tttcatcgat gtccgcatca
     5221 tcgacggctc gatcaacggc accgccgccc tgatcggcgc cggggcccgg gccctcagcg
     5281 ccctccagac cggtgttgtc cagaactacg ccctggcctt tgtcttcggc gtgatcgcgc
     5341 tcctcgtcta tctctttgtc taggtgaagc agttgcccat tattccggag cctcttcatg
     5401 agtgatttga gcaccatgcc catactgacc atcctcacct ttctgcccct ggcaggcgcg
     5461 ctcgtgctgc tcttcgtgcc gcggcagagg gaagcgctga tccgctggtt caccctggtg
     5521 ttgactctgg cggtctttct gctctcgctg ccgcttttca ccgccttcga cggcgccaat
     5581 cccggcttcc agttcgagga gaaccggagc tggataccct cggccgggat cggctaccat
     5641 ctcggcgtcg acggcatcag cctgctgatg gtcatgctca ccgcctttct gaccccactg
     5701 gtgattctct ccagctggcg ttccgtgacc aaaaaggtca aggaatacat gatcatgttc
     5761 ctggtgctcg agactgccat gctcggggtc tttctgagcc tggatatggt cctcttttac
     5821 ctcttctggg aggggatgct catcccgatg tatttcatca tcggggtctg gggcggggag
     5881 cgccggctct atgcggcgat caagtttttc ctcttcacca tgttcggcgg ggtgctgatg
     5941 ctggtcgcca tcctcgtcct ctatttcatg cacggcggcc agagcggact gtacaccttc
     6001 gactatctcg ccctcagcca gatgctcatc gccagcaaga gccaggtctg gctcttcttc
     6061 gcgttcggca tcgcctttgc gatcaaggtg ccgatgttcc cctttcacac ctggctgccg
     6121 gatgcccatg tggaggcgcc aaccgggggc agcgtcatcc ttgccggcat cctcctcaag
     6181 atgggcacct acggttttct gcgcttctgc atccccctct ttccgactgc cgcacagcag
     6241 tacggctggc tgatcatgct gctggcggtg atcggcatca tctacggggc cctggtgagc
     6301 tgggtacagc ctgacctgaa aaagctggtc gccttttcct cggtcagcca tctcggtttc
     6361 gtcatgctgg ggcttttcgc cctcaccctg cagtcggtgc aggggggatt gatccagatg
     6421 gtcaatcacg gcctctccac cggcgccctc ttccttctgg tcgggatgat ctatgagcgc
     6481 cgtcacaccc gcctgatcgc cgatttcggg gggctggcgc ggcagatgcc ggtttttgtg
     6541 acctttttcc tcatcatcac cctctcctcg atcggcctgc cgggtttgaa cggttttgtc
     6601 ggcgagttca tgatcctcct cggcaccttc gaggtcaaca ggctcctggc ggtgctggcg
     6661 accaccggcg tgatcctctc ggcggtctat atgttgacca tgtttcagcg cgtcaatttc
     6721 ggcaagctcg acaaacccga gaatcaggag ctgaa
//
LOCUS       DAOPWU010000181         6688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_11394_length_6688_cov_5.323232, whole genome shotgun sequence.
ACCESSION   DAOPWU010000181 DAOPWU010000000
VERSION     DAOPWU010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11394_length_6688_cov_5.323232"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKM23_09750"
     CDS             <1..329
                     /locus_tag="PKM23_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91797.1"
                     /translation="PVHDRIHFGEVLAFRQPERERLAVHRVVGRGRRGFLLKGDNGCH
                     CDGWVTREKIIGRVTRVEHRGREVRIGLGIERILIAGLSRLRLLRPLVFPVRWLLRPV
                     YKRFFA"
     gene            326..1444
                     /locus_tag="PKM23_09755"
     CDS             326..1444
                     /locus_tag="PKM23_09755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY91798.1"
                     /translation="MTHTSSIEIQDFALWQKLEAKRALFSFDLDLTARCNLNCRHCYI
                     NLPAGDEAARARELSLEEISHIADQAIGLGALWCLLTGGEPLLRPDFAEIYMMLKRKG
                     LLISLFTNATLIRPEHIALFSKYPPRDIEVTIYGASAVTYERVTRRPGSFVYFVAGLD
                     ALFAAGIKVRLKAMALRSNLEDMEAIAAFGRARTKDFYRFDPQLHLRYDRDPVRNEEI
                     KAERLTPAEVTAMERADAERFGSMLKGCDELINEAFTHAGCNHLFHCGAGNGSCAIGY
                     DGTFRLCQSLWAPGTTANLREVPLRQAWEELVPRVRDMRSQDPQFLASCRKCAIVNLC
                     LWCPAHAWLETGRMDGATPFFCQVAHARAAAILEKTGE"
     gene            1631..2881
                     /locus_tag="PKM23_09760"
     CDS             1631..2881
                     /locus_tag="PKM23_09760"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HNY91799.1"
                     /translation="MTIRRTFALFFFIGSALLQAQTDEYEALYGRLAYHGFENLRIGA
                     AADSGLAIAFENRVYRHDLKAAGEILAIASETDSTWRRIYLIPCSRGVARGQIAVDGD
                     AYRSYMAGAMDEKAFAKTLAVGEATEQPRTSVLSWKNRSFLKVDANITIGHQIQLGQY
                     DDRVKLYGELQPGLRSQIWHGTAVQADAFVPLFDEISVFDSGARLGRLGVSQLFRLPH
                     DSYAALQGGIFMPERWGLSGEVAKFWLERRILTGLKLDRTGFFFHTEGKFYYSPMDSL
                     TYTVYAQYYLPGMDMMAGIDYSKYLLGDKGVKFSLRRTFAETDIGLFYAITDYDRFGG
                     VQIRFPLPTQRRMKPARVRLTWPNQYGMTYQATSEATRVEGILQTGLMVDSGFAIAEF
                     IRHLTPAHVISSVKGWRKGADSAY"
     gene            2917..>4527
                     /locus_tag="PKM23_09765"
     CDS             2917..>4527
                     /locus_tag="PKM23_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91800.1"
                     /translation="MKRIAKTGLVLALLVLAFALSCEQVTDPLTVSQFQTANNATLFN
                     AATGVQKSVFYVGENVFLTINDLYPNEQTDIQIVRRSDGNIIKRMLVISDENGSIVDL
                     PVWFLNGFRKPGELAGDHKFVAHIEQPGVGHDWKIFSIPFEARDQITPDPGMGAVTET
                     GEPLGHAVLSGQPVWIAGINLGAHVTYKFLLVNDRSNYTLGDVFTDVSDNGIETDRTE
                     RDGTFEPTKISNSAIPGLYDLVADREPFGIYNTGDWIGDLKHPGLVVQLPASGAHIIQ
                     DIACDAWGTHKNQFDSLEVIYGQVTAMTRPQGASEFVTVFIAPHKPVWMKGDKLFSLR
                     TVGTHEMPVQCLWNGYWGYLPVIRIHGGALATDANPIKMWPGEYDVILDVDRNNVYDP
                     GVDILDGGTAPGFVVPGKVPPVRFGASADLDFLGRFEGDPNIWGYFRDHIKTPVWGVL
                     VDSLGGFISGVPIKFSITEGSGTLSTATATTGLDGAAFTVFSGLSWGKSATVKLEARV
                     KDKDYVRHVLIFRKIPWQHNQGIIVHNQG"
     assembly_gap    4528..4627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4628..5029
                     /locus_tag="PKM23_09770"
     CDS             <4628..5029
                     /locus_tag="PKM23_09770"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HNY91801.1"
                     /translation="VRLLREKGWRPAVTVGTQDFFAIKQLKWETATAQHFGALYLVAS
                     RHDTLRGVPLHFHLGYGPDWLVARTKMLVGLFGGVSYSPHRMVTLLAEYDAECFNAGL
                     RLQPFPFLQAHLGLWKLKEVTANLAFTVHLQ"
     gene            5159..>6688
                     /locus_tag="PKM23_09775"
     CDS             5159..>6688
                     /locus_tag="PKM23_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY91802.1"
                     /translation="MTPRLRSLRKITTRQLREELLPNILRSLGLVWRASPMLFIVSTL
                     LLLVQSVLPLGILYASKLILDGVASALQTGTAAATPPALILLASLVGGLVFLQVVASS
                     IHSWLQSHQSAMVQENIMEKLHHKSVEIDFAYYENPEFFDTLHRAQHQAIYRPSQLVG
                     RLQNIIQQSLTLAGLVGLLFLFHWSIPLLLLLTLLPAFLLRLHFADVLFEWRQKSSSL
                     ERKSYHYHWLLTDKAWAKEVRALGLGRFLRRRYLAIQRRLRVEQFRINGRQTRGTLMA
                     QLLATAVAYGFYLVLIGKTLAGAITLGSLVMYFQAFQQALNALRNLFTGIGDIYEDTR
                     FVSQLYDFFNLEPVMPRTRPVQPVPSPVRRGLSVEGLAFRYPGSERWALREVSFSAAP
                     GERIALAGANGSGKSTLMKLICRLYDPAEGRILLDGVDIRDFELTGWWRNLAVMFQDF
                     EEYNLSARENIWLGDIRLPLHSPAVEQAAQRSGAAGVIEGLPKGYETMLGKWFDEGEE
                     LS"
BASE COUNT         1182 a         2163 c         1912 g         1331 t
ORIGIN      
        1 cgccggtcca cgaccgcatt catttcggcg aagtgctcgc gttccggcag ccggagcgtg
       61 agaggctggc tgtgcaccgg gtcgtcggcc gcggccgcag gggttttctg ctcaagggcg
      121 acaacggctg ccactgcgat ggctgggtga cccgggagaa gatcatcggc cgggtcacca
      181 gggtcgagca ccgcggccgg gaggtgcgca tcggtttggg gattgagcgt atcctaatcg
      241 ctggattgtc gcggctcagg ctcctgcgtc ctctggtatt tcccgtgcgc tggctgctgc
      301 ggccggttta taaaaggttt tttgcatgac ccatacttca tcgattgaga ttcaggattt
      361 cgctctttgg caaaaactcg aggccaaacg cgcccttttc agctttgacc tggatttgac
      421 cgcgcgctgc aacctcaact gccgccactg ctatatcaat ctgccggcgg gggatgaggc
      481 cgcccgggcc agggaactct ccctcgagga gatcagccat atcgccgatc aggcgatcgg
      541 actgggcgct ctctggtgtc tgctcaccgg cggggaaccg ctgctgcggc ccgatttcgc
      601 cgagatctat atgatgctca agcgcaaggg gctgctgatc tcgctcttca ccaatgccac
      661 cctgatccgg ccggagcaca tcgcgctttt ttcaaaatac cccccgcgcg acatcgaggt
      721 gaccatttac ggcgcttcgg ccgtaaccta cgaacgcgtc actcgccggc ccggctcctt
      781 tgtatatttt gtcgccggcc tggatgccct ttttgccgcg ggcatcaagg tgcggttaaa
      841 ggccatggcc ctgcgctcca atctggagga tatggaggcg atcgccgcct tcggccgggc
      901 tcgaaccaag gatttctacc gcttcgatcc ccagctccat ctgcgctacg atcgcgatcc
      961 ggtgcgcaac gaggagatca aagcggagcg gctcaccccc gcagaggtga cggccatgga
     1021 gagggcggac gctgagcgtt tcggttcgat gctgaagggc tgcgatgagc tgatcaacga
     1081 ggctttcacc catgccggct gcaaccatct ctttcactgt ggcgccggca acggcagctg
     1141 cgccatcggc tacgacggca ccttccgcct ctgccagtcg ctctgggccc cgggcaccac
     1201 ggccaatctg cgtgaggtgc cgctgcgcca ggcctgggaa gagctggtgc cgcgggtgcg
     1261 ggacatgcgc tcgcaggatc cgcagttcct ggccagctgc cgcaagtgcg ccatcgtcaa
     1321 cctctgcctc tggtgcccgg cccacgcctg gctggagacc ggccgcatgg acggggcgac
     1381 cccctttttc tgccaggtcg cccatgcccg cgcggcagcg atactagaaa aaactggcga
     1441 ataataaatt attttgttgc ttttttgtac attattaatt aatattagtt aataggggac
     1501 gtgtggggaa gagtatctca atttcacggt tatactgcct ttgttccgca tccattgctc
     1561 gccaacctga ccgtccaaca ccccttttaa ctctgctctg caccgtatca gcaactttgc
     1621 cgggacatcg atgaccattc gacgcacctt tgccctgttc tttttcatcg gcagcgcgct
     1681 ccttcaggcc cagacggatg agtacgaggc tctttacggg cgcctggcct atcacggttt
     1741 cgagaacctg cgcatcggcg cggccgccga cagcggtctg gccatcgctt tcgagaaccg
     1801 cgtctaccgc catgatctca aggcggccgg cgagatcctc gccatcgcca gcgagaccga
     1861 ttcgacctgg cggcgcatct atctcatccc ctgcagccgc ggcgtggccc gcggccagat
     1921 cgccgtcgat ggcgatgcct atcgcagcta tatggccgga gctatggatg agaaggcttt
     1981 tgccaaaact ctggcagttg gagaggcgac cgaacagccg cgcacatcgg tcctctcctg
     2041 gaaaaaccgt tctttcttaa aagtcgatgc caacatcacc atcggccatc agatccagct
     2101 cgggcagtac gatgatcgcg tcaagcttta cggcgagctg cagcccggtc tgcgcagcca
     2161 gatctggcac ggcaccgcgg tccaggccga cgcctttgtc ccgctcttcg acgagatcag
     2221 cgtctttgac agcggcgcac ggctggggcg cctgggcgtc agccagctct tccggctgcc
     2281 ccacgacagc tatgcggccc tgcagggcgg gatctttatg ccggagcgct ggggcctctc
     2341 gggcgaggtg gccaaattct ggctggagcg caggatcctg acgggcctga aactggatag
     2401 gaccggcttc tttttccata ccgagggcaa gttctattac tcgcccatgg acagcctgac
     2461 ctatacggtc tatgcccagt attatctccc cggcatggat atgatggcgg ggatcgatta
     2521 cagcaagtat ctgctcggcg ataaaggggt caaattcagc ctgcggcgga cttttgccga
     2581 aaccgatatc gggctctttt acgccatcac cgactacgac cgcttcggcg gggtgcagat
     2641 ccgcttcccg ctgcccacac agcggcggat gaaacccgcc cgggtgcgcc tgacctggcc
     2701 caaccagtac ggcatgacct atcaggccac cagcgaagcg acccgggtgg agggcatatt
     2761 gcagaccggc ttgatggtgg attcaggatt cgccatcgcc gagtttatca gacatctcac
     2821 ccctgcacat gtcatcagca gcgtgaaggg gtggcgcaag ggggctgact cagcctattg
     2881 attaccaaaa ccatatctaa cagggaggaa acaaggatga aacgaatcgc aaaaacaggc
     2941 ctggtgctgg cgctgctggt attggcgttt gcactgagct gcgaacaggt gaccgatcct
     3001 ttgaccgttt cgcagttcca gaccgccaat aacgccacgc tcttcaatgc cgccaccggc
     3061 gtgcaaaaga gcgtctttta cgtcggcgag aatgttttcc tgaccatcaa cgatctctat
     3121 cccaacgagc agaccgacat ccagatcgtg cgcaggagcg atggcaatat catcaagcgc
     3181 atgctcgtca tctcggatga aaacggctcg atcgtcgatc tgccggtgtg gttcctgaac
     3241 ggcttccgca agcccggcga gctggccggc gaccacaagt tcgtggcgca catcgagcag
     3301 cccggcgtgg ggcacgactg gaagatcttc agcattccct tcgaagcccg cgaccagatc
     3361 accccggatc ccggcatggg cgctgtgaca gaaaccggtg agccgcttgg ccatgccgtt
     3421 ctctccggac agccagtctg gatcgccggc atcaacctgg gcgcccatgt cacctacaag
     3481 ttcctgctcg tcaacgatcg cagcaattac accctcggcg atgtcttcac tgacgtcagc
     3541 gataacggca tcgagaccga ccgcaccgag cgcgacggca ccttcgagcc caccaaaatc
     3601 agcaattcgg ccattcccgg cctgtatgat ctggttgccg accgcgaacc cttcgggatc
     3661 tacaataccg gcgactggat cggcgatctc aaacacccgg gcctggtcgt tcagttgccc
     3721 gccagcggcg cccatatcat ccaggatatc gcctgcgatg cctggggcac ccacaagaac
     3781 cagttcgact ccctggaagt gatctatggt caggtcacgg cgatgacgcg gccgcagggc
     3841 gccagcgagt tcgtcaccgt ctttatcgcg ccgcacaagc cggtctggat gaaaggcgac
     3901 aagctcttca gcctgcgtac cgtcggtacc cacgagatgc cggtgcagtg cctctggaac
     3961 ggttactggg gctacctgcc ggtgatccgc atccacggcg gcgcactggc gacagatgcc
     4021 aacccgatca agatgtggcc cggcgagtat gatgtcatcc tcgacgtcga ccgcaacaat
     4081 gtctacgatc ccggcgtcga tatcctcgac ggcggcaccg ctcccggttt cgtggtcccc
     4141 ggcaaggtcc cgcctgtccg cttcggcgcc tctgctgatc tcgatttcct cggccgcttt
     4201 gaaggcgatc ccaatatctg gggttacttc cgcgatcata tcaagacccc ggtctggggc
     4261 gtgctggttg attccctcgg cggtttcatc agcggcgtgc cgatcaaatt ctcgatcacc
     4321 gaaggctccg gtactctgag caccgccacc gccaccaccg gccttgacgg cgccgccttt
     4381 accgtcttct cgggcctgag ctggggcaag agcgccacgg tcaaactcga agcgcgcgtg
     4441 aaggacaagg attatgtccg ccacgtgctg atcttccgca agatcccctg gcagcacaat
     4501 cagggcatca tcgtccacaa tcagggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnngtg cgcctgctgc gcgagaaggg ctggcgcccg gccgtgaccg tcggcaccca
     4681 ggactttttc gccatcaagc agctcaagtg ggagaccgcc accgcccagc atttcggcgc
     4741 tctctatctg gtcgcctcgc ggcacgatac cctgcgcggg gtgccgctcc acttccatct
     4801 cggttacggc ccggactggc tggtcgcccg gacgaaaatg ctggtcggtc tcttcggcgg
     4861 agtctcttat tctccccacc gcatggtgac cctgctcgcc gagtacgacg ccgagtgctt
     4921 caacgccggc ctgcgcctgc agcccttccc ctttctccag gcccatctcg gcctctggaa
     4981 gctgaaggag gtgaccgcta atctcgcttt tactgtgcat ctgcaataat ccccccctcg
     5041 cccgctcgcg ccccaagata tcgctgccac ctgcccccgc tcgcactacc cggcggaatt
     5101 ttctccccgt cctggttgtt gcctcctata gaaaccgacc ctttgaaagc cgatcctgat
     5161 gaccccacga ttgcgctccc ttcgcaagat cacaacccgg caactgcgtg aggaattact
     5221 ccccaatatt ctccgttccc tcggactggt ctggcgggcc tcgcccatgc tcttcatcgt
     5281 cagcaccctg ctgctgctgg tacagagcgt gctgcccctg ggcattctat atgcgagcaa
     5341 gctgatcctc gacggggtcg cctccgccct gcagacgggc acggccgccg ccacgccgcc
     5401 cgcgctcatc ctgctcgcct cgctggtggg gggactggtc tttttgcagg tggtcgcctc
     5461 cagcatccac tcctggctgc agagccatca gtcggccatg gtgcaggaga acatcatgga
     5521 aaagctccac cacaagtcgg tggagatcga tttcgcctat tacgagaatc cggaattttt
     5581 cgacactctg caccgggccc agcaccaggc catctaccgc ccctcccagc tggtcgggcg
     5641 gctgcagaac atcatccagc agagcctgac cctggccggg ctggtcggcc tcctcttcct
     5701 cttccactgg tcgatccccc tgctcctgct gctcaccctg ctgcctgctt ttctcctccg
     5761 cctccatttc gccgatgtgc ttttcgagtg gcggcaaaag agcagcagcc tcgagcgcaa
     5821 gagctaccat taccactggc tgctgacaga caaggcctgg gccaaggagg tgcgggcgtt
     5881 gggcctgggc cgtttcctgc gcaggcgcta cctggccatc cagcggcggc tgcgggtcga
     5941 gcagtttcgc atcaacggcc gccagacccg cggcactctt atggcccagc tcctggcgac
     6001 ggcggtggcc tatggctttt acctggtact gatcggcaaa accctggccg gggccatcac
     6061 cctcggcagc ctggtgatgt attttcaggc ctttcagcag gccctcaatg ccctgcgcaa
     6121 cctcttcacc ggtatagggg acatctacga ggatacccgc ttcgtttcac agctctacga
     6181 ctttttcaat ctcgagccgg tgatgccccg cacccggccg gtgcagccgg tgccgtcgcc
     6241 ggtccggcgc ggcctcagcg tcgaggggct cgccttccgc tatcccggca gcgaacgctg
     6301 ggccctgcgc gaggtctctt tcagcgccgc ccccggcgaa cgcatcgccc tggccggggc
     6361 caacggctcg ggtaagagca ccctcatgaa gctgatctgc cggctttacg acccggcgga
     6421 gggacggatc ctgctcgacg gggtcgacat ccgcgacttt gagctgaccg ggtggtggcg
     6481 taatctggcg gtgatgtttc aggatttcga ggagtacaat ctcagcgcgc gcgagaacat
     6541 ctggctgggg gacatccgcc tcccgctgca cagtccggct gtggagcagg cggcgcagcg
     6601 ctcgggcgca gccggcgtga tcgaggggct gcccaagggc tatgaaacca tgctcggcaa
     6661 gtggttcgat gagggggagg agttgagc
//
LOCUS       DAOPWU010000182         6688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_11395_length_6688_cov_5.286296, whole genome shotgun sequence.
ACCESSION   DAOPWU010000182 DAOPWU010000000
VERSION     DAOPWU010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11395_length_6688_cov_5.286296"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3693)
                     /locus_tag="PKM23_09780"
     CDS             complement(<1..3693)
                     /locus_tag="PKM23_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNY91803.1"
                     /translation="MKQKATFVIAVLTGVWSLAWGQPNHTQELFRYDLTTSSTPVAQL
                     DNFSGSFSSTGWRPTSANGRLKLYLADYMPYEGTLEVQVKGLSADVVTKDWIPFSIWS
                     RGRGAFYQRDGVSYPSEGSYAFIKTDTSKVSGSSVQFKLFTKSQYDPVRANHQTADVP
                     GYSYNASTTYTLRFIWRPGTIWYQVWAGGSKLVEASTPWVMQGEAFSFIFLGKNYEYS
                     SMTGVTYSNLVLKGPQKAISFADVTRSAGVAVDTTLNGQGVSWADLDNDGKEDIYASY
                     FNQANRYFAALPDSDKFSESASAAGLADTGPSFAMASADFNGDGRLDAFLANYGGSDR
                     LFINQGGSFSDQSGSWNINTATSSSINPLAFDLENDGDIDLFVANNGAANVLYINQNN
                     SSFTRVDLSSLPFGSGARAAAGDINKDGYVDIFFPRRNAAAVLLINNKAGGFTDQASA
                     YGLDVNTDPNAPTLADLDNDGWLDLLLSVASAGDAKPQVLYYRNSGSGSFSKAGTIYI
                     ECYGAIVGDVDNDGFQDLYLIKRNRYSAESSDYGSRLYRNTSSPGSISFAEYTGTGLE
                     AIFQDGRGGAMADYNSDGKIDIFGTAKGTTGSNGLNYGRNFLFRNTTASENGFLLVRV
                     LDKKKLLGYLGAKLELFEQSGVSGTRLGYREISSIQGYQSQPSRMAHFGMGSNTAGVL
                     RVTLPDGQVLTRTVNSNTLVEIDPTGGDAASFILVKGNNQTGVAGTVLNDSLTVRVYA
                     KADEQRPLAGHSVTFTVSQGDGTVNGGASASVQTDLHGLARAAFKLGRTAGAANNGVR
                     ITSLDKSGAQIINLSAPGTPAAPIEIVASANPGPAAKMVKVGGEGQSGYLNEVLATPI
                     KVRVTDEYGNIVSGYTVTFNVATGGGGFGTSGSSPMTAVTGPDGTASANWRLGMLLGV
                     QTLQVYGAFNTSTPALFSASASEPLRRLTYESGDRQSARVGTTPDAALVVRLRDYQGN
                     AIAGVAVRFAVIAGGGKINGQTAVDINTSPDGLASVKPNVGTAVGDTNNVFQATSVGA
                     AGTVTFKISATAGAATRLVETSGNGKSGKVGRILTAPFVVRVTDANANPVANHPVDFN
                     VITGGGSINGQNSARIATDKSGYASAWYRLGTATGANTVTATASGLTGSPITFTAVAE
                     AGSPALLYKVSGDNQKGTFGAPLAQPLIVALSDSFSNPIANHPVQFLVSRGNGAVNGL
                     TLATIQTNPTGQASVVFSLGDDDYINQ"
     gene            complement(3724..>6688)
                     /locus_tag="PKM23_09785"
     CDS             complement(3724..>6688)
                     /locus_tag="PKM23_09785"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY91804.1"
                     /translation="LSGYAVPNHPVTFWVRRGGGHLEGQPEVTRISDANGVVAVTATL
                     GSAYGIANNVFEAQSFQGSGVHLSGSPVRFYVSARKSLAHKMDGVSGGGASAQAGEVL
                     PFPLAVKIVDKAGQAVAGQEVLFKVVKGSGKLGVGALSSATVQSNAAGIAQISFRLGV
                     ELGSQTHIVQASANDGVSALVNSPLTMAISAPYGQPDTSASTVTITSPVTADGVAECA
                     VRVRLVDGAGNGVPGERVTLLVSGDGNRIVQPEAATDGAGETEGYVSSTVAGSKTIRI
                     YISSKERYLGVAPVVQFKAGTPQRISAVDGDFQSGYIQSALEKPVVVRLEDFYGNPVA
                     GAELIFTPAVGSGEVSPGQTIMTDQAGRAQATWILGSSVGEQKLDVRAAGTAVLRTIT
                     ATALLPQQIQLEKSGGDNQFAAPGTLFPDSLTVRVVDDHGAPIFGVGITFAVLQGSAS
                     LGTTSAVLSDRYGLARMRVAAGAEPGVVRIRALAGSGDPVDFTLSVSRSIPALITRIY
                     GDSLSDEAGKSLYPLMVQVTDLAGNPVANVPVHFETLTGGAAIVEPQPLYTDNDGQAA
                     ATLRLGTTAGVYLCQASSSHVNGSPVAFTLFATPGAAAEMVVHEGDQQPARALKTLPA
                     PIKVRLTDRFGNGVPGQTVQFAPLAGGGSVAPAVVITDGAGIAAASWTLGAGGTQTLQ
                     ARSPSLPGKEALFSALLIENLEPVIYAVDDTTVLETINLVFEVRAVDPDGGAVTLSVE
                     NLPAGATFDPVNSRLFIWRPGHDQQGRHLIRFTARDENGGVTSKTTTVRVQNLNRPPV
                     IYAYQPESFYIQAPAFKPATFSVQSSDQDGDALTCRWRLNGRLVGEGDTLTILTGTAL
                     PAQFTLTATVYDQSDSVAQAWSVQQPTGVASGRDEALLPTRSRLVQNYPNPFNPETTI
                     AWELAAPQEVEIRIYNMSGQAVRTLYAGRAAAGYHRLVWNGQSDSGETVPSGVYPCVL
                     RGEGLHQMIKLVFLK"
BASE COUNT         1183 a         2023 c         2248 g         1234 t
ORIGIN      
        1 ctggttgatg taatcatcat cgcccaggga aaagaccacc gaggcctgac ccgtgggatt
       61 ggtctggatt gtagccaggg tgaggccgtt gaccgcgccg ttgccgcggc tgaccaggaa
      121 ctgcacggga tggttggcga tgggattgct gaaggaatcg ctcagtgcga cgatgagggg
      181 ctgcgcgagg ggcgcgccga aggtcccctt ctggttgtcg cccgagacct tgtaaagcag
      241 ggcggggctt ccggcctcag cgactgccgt gaaggtgatc ggcgatccgg tcaaaccgga
      301 ggccgtcgca gtgacggtgt tggcgccggt ggcggtcccc agccggtacc aggccgaggc
      361 gtatccgctc ttgtcggtgg cgatgcgggc cgaattctgg ccgttaatcg agccgccccc
      421 ggtgatgaca ttgaaatcga cgggatggtt ggcgaccgga ttggcgttgg cgtcggtcac
      481 gcgcaccaca aagggggcgg tgaggatgcg cccaaccttg ccgctcttgc cgttgcccga
      541 ggtctcgacc agcctggtgg ccgccccggc cgtggccgag atcttgaagg tgactgtgcc
      601 ggccgcgccc accgatgtgg cctgaaaaac gttgttggtg tctcccaccg ctgtgcccac
      661 attcggcttg accgaggcca gcccatcagg ggaggtgttg atatccaccg cggtctggcc
      721 gttgatcttg ccgccgccgg cgatcacggc aaagcgcacc gcaaccccgg cgatggcgtt
      781 gccctgataa tcgcgcaggc ggacaacgag cgcggcatca ggggttgtgc ccacccgggc
      841 gctctgacgg tcgcccgatt cgtaggtcag acggcgcagg ggctccgaag ccgaggctga
      901 aaagagtgcg ggtgtgctgg tgttgaatgc cccatagacc tgcagggtct gcacgccgag
      961 gagcatgccc agacgccagt tggccgatgc cgttccgtcc gggccggtga cggcggtcat
     1021 gggtgagctg ccgctcgtgc cgaagccccc tcccccggtg gcaacgttaa aggtaaccgt
     1081 ataaccgctg acgatattgc cgtactcatc ggttaccctg accttgatcg gggtggcgag
     1141 cacctcattg aggtagccgc tctgtccctc tccccctacc ttcaccatct tcgctgcagg
     1201 gccgggattg gccgaggcga cgatttcgat gggggccgca ggggttccgg gggctgagag
     1261 attgatgatc tgggcgccgc tcttgtcgag cgaggtgatc cggacgccgt tattggccgc
     1321 ccccgcggtc ctccccagct tgaaggccgc tcgcgccagg ccatgaagat cggtctggac
     1381 ggaggccgaa gcgccgccgt tgacggtgcc gtcgccctgg gagaccgtga aggtgacgct
     1441 gtggcccgcc agagggcgct gttcatccgc cttggcgtag acccgcaccg tgagggaatc
     1501 gttgagcacc gttcccgcca ccccggtctg gttgttgccc ttgaccagga tgaaggaggc
     1561 ggcgtccccg ccggtgggat cgatctcgac cagggtattg ctgttgacgg tgcgggtgag
     1621 gacctgtcca tccggcaggg tgacgcgcag cacgcccgct gtgttggagc ccatgccgaa
     1681 atgggccatg cggctgggct ggctctgata accctggatc gaactgatct cgcgatagcc
     1741 caggcgggtg ccgctgacgc cgctctgttc aaagagctcg agcttggcgc cgaggtagcc
     1801 gagaagcttt ttcttgtcca gcacgcggac caggagaaag ccgttctcgc tggcggtcgt
     1861 gttccggaaa aggaagttgc ggccgtaatt cagaccattc gagcctgtgg tgcctttggc
     1921 cgtgccgaag atatcgatct tgccgtcgct gttgtaatcg gccatagctc cgccgcggcc
     1981 gtcctgaaag atggcctcca ggccggtgcc ggtatattcc gcgaaactga tgctgcccgg
     2041 cgaagaggtg ttgcggtaga ggcgcgagcc gtaatctgaa ctctcggcgc tgtaccggtt
     2101 gcgtttgata agataaaggt cctgaaagcc gtcattatcg acgtcaccga cgatggcgcc
     2161 atagcattcg atgtagatgg tgccggcctt ggagaaggaa ccgctgcctg agttgcggta
     2221 atagagcacc tggggcttgg cgtcaccggc gctggccacg gagaggagca ggtcgagcca
     2281 gccgtcgttg tcgagatcgg caagagtcgg cgcattcgga tcggtattga catccagccc
     2341 gtaagcggag gcctgatcgg tgaatcctcc ggccttgttg ttgatcagca gcaccgcagc
     2401 ggcattgcgg cgggggaaaa agatgtcgac atagccgtcc ttgttgatgt cgcccgcagc
     2461 ggcgcgcgcc ccggaaccga agggcaggct gctcagatcg acgcgggtga agctgctgtt
     2521 gttctgattg atatagagga cgttggccgc cccgttgttg gcgacgaaga gatcgatatc
     2581 cccgtcattc tccaggtcga aggccagggg attgatggag ctgctggtag cggtattgat
     2641 attccaggat ccgctctggt cactgaagga gccgccctga ttgatgaaga ggcggtcgga
     2701 gccgccgtaa ttggccagaa aggcatcgag acggccgtcg ccgttgaaat cggcgctcgc
     2761 catcgcaaat gaggggccgg tgtcagcaag cccggcggcc gaggccgatt cggagaactt
     2821 gtcgctgtcc ggaagcgcgg cgaagtagcg gttggcctga ttgaaatagg aggcgtagat
     2881 atcctctttc ccgtcgttgt ccagatcggc ccagctcacc ccctggccgt tgagagtggt
     2941 atcgacggct acgccggcgg atctggtcac atccgcgaac gaaatcgcct tctgcggtcc
     3001 cttcaggacc aggttgctgt aggtaacccc ggtcatggag gagtattcat agtttttgcc
     3061 cagaaagatg aaggagaaag cctcgccctg catgacccag ggggtggagg cttcgacgag
     3121 cttgctcccc cctgcccaga cctggtacca gatcgtgccg ggcctccaga tgaaacggag
     3181 ggtgtaggtc gtggaggcgt tgtaggagta tccaggcaca tcggcggtct gatggttcgc
     3241 ccgcaccgga tcgtactgcg atttggtgaa gagcttgaac tgcacgctcg atcctgagac
     3301 cttggaagta tcggttttga tgaaggcata actgccctcc gagggatagc tcaccccgtc
     3361 gcgctggtaa aaggcgccgc gcccgcgcga ccagatcgag aaggggatcc agtccttggt
     3421 gacaacatct gcggagagcc ccttgacctg cacctcgaga gtgccctcat agggcatgta
     3481 atcggccagg tagagcttga gccggccatt cgcacttgtg ggccgccatc cggtcgagga
     3541 gaaggagccg ctgaaattat cgagctgggc gaccggagtc gaggaggtgg tcagatcgta
     3601 ccggaaaagc tcctgggtat ggttcggctg accccaggcc agactccata ctcccgtcag
     3661 aactgcgatt acaaaagttg ctttttgctt caacataacc tcacccttga aaaggttaaa
     3721 ccgtcacttg agaaatacca gcttgatcat ttgatgcagg ccctcgccgc gcagcacgca
     3781 gggatagacc ccggagggca ccgtttcacc gctgtccgac tggccgttcc agaccagccg
     3841 gtgatagccg gccgcagccc tgccggcgta gagcgtccgg acggcctggc ccgacatatt
     3901 gtagattctg atctcgactt cctgaggcgc tgccagttcc catgcgatcg tggtctcggg
     3961 attgaagggg ttgggataat tctgcaccag gcggctgcgc gtgggcagca acgcctcatc
     4021 ccgaccggag gcgacgcccg taggctgctg cactgaccag gcctgcgcca ccgaatcgct
     4081 ctggtcgtat acggtcgcag tcagggtgaa ctgggccggc agggcagtgc cggttaggat
     4141 agtcagggta tccccctctc ccaccagccg cccgttgagc cgccagcggc aggtcagggc
     4201 atctccgtcc tgatcggaag actgcacgct gaaagtcgcg ggcttgaagg ccggggcctg
     4261 gatgtaaaag gactcgggct gataggcata gatcaccggg ggccggttga ggttctggac
     4321 ccggaccgtg gtggtcttgc tggtcacccc cccgttttcg tccctcgccg tgaaacggat
     4381 gagatggcgc ccctgctgat catgtcctgg ccgccagatg aagagcctgc tgttgacggg
     4441 atcgaaggtg gcgccggccg gcagattctc aacggaaaga gtgaccgctc cgccgtccgg
     4501 atcgaccgcc ctgacctcga aaaccagatt gatggtctcc agcacggtgg tgtcgtcgac
     4561 ggcgtagatg accggctcga gattctcgat caagagggct gagaagagcg cctccttgcc
     4621 gggcagggat ggggatctgg cctgcagggt ctgggtgccg cctgcgccca gggtccaact
     4681 ggccgctgcg atgcccgctc cgtcggtgat cacgactgcc ggggcgaccg agccgccgcc
     4741 ggcgaggggg gcgaactgga cggtctggcc cgggacgccg ttgccaaagc gatcggtgag
     4801 gcgcaccttg atcggggcgg gcagagtctt gagggcgcgg gcgggctgct gatcgccttc
     4861 gtggaccacc atttcggccg ctgcaccggg agtggcgaag agggtaaagg cgaccggcga
     4921 gccgttgaca tggctgctgc tggcctgaca gagatagacc ccggccgtgg tgccgaggcg
     4981 aagcgtcgcc gccgcctggc cgtcattgtc agtatagagg ggctgtggct cgacgatcgc
     5041 ggccccgcca gtcagggttt cgaaatggac agggacattg gcgacgggat tgcccgccag
     5101 gtcggtaacc tgcaccatga gcggataaag gctcttgccg gcctcgtctg agaggctgtc
     5161 gccgtaaatc cgggtaatca gcgccgggat cgaacgggat accgagagtg taaagtcgac
     5221 cgggtcgcct gaacccgcca gtgcgcggat tctgaccaca ccgggctcgg cccccgccgc
     5281 gacgcgcatt cgcgccagtc cgtaacggtc gctgagcacg gcgctggtgg tgcccaggct
     5341 ggctgaaccc tggagtacgg cgaaagtgat ccccaccccg aaaatggggg cgccatgatc
     5401 atccaccacc cgtacggtga ggctgtccgg aaaaagggtg cccggagccg cgaactggtt
     5461 atcccccccc gacttttcaa gctgtatctg ctggggtaaa agggcggtgg cggtgatggt
     5521 gcgcaggact gcagtgccag cggcgcgcac atcgagcttt tgttcgccga cgctggagcc
     5581 gaggatccag gtcgcctgcg cgcgcccggc ctgatcggtc attatcgtct gcccgggtga
     5641 aacctcgccg ctgcccacgg ccggcgtgaa gatcagctca gcgccggcga ccggattgcc
     5701 gtaaaaatcc tccagccgca ccactacagg cttttcgagg gccgactgga tgtagccgga
     5761 ctggaaatcg ccatccaccg cgctgatgcg ctggggagtg cccgccttga actgcacaac
     5821 cggggccacg ccgagatatc tctccttgct ggagatatag attctgatcg ttttgctgcc
     5881 ggcgaccgtc gagctcacat agccctcggt ctcgcccgcg ccgtccgtag ccgcctcggg
     5941 ctggacgatg cggttgccgt cgccgctgac cagcagggtg acccgctctc cgggaacacc
     6001 attccccgca ccatcgacca ggcggacgcg cacagcacat tcagcgaccc cgtcagccgt
     6061 gaccgggctg gtgatcgtca ccgtcgaggc actggtgtcg ggctgcccgt aaggggcgct
     6121 gatggccatg gtgagcggcg aattgaccag ggccgaaacc ccgtcgttgg cgctggcctg
     6181 cacgatatgg gtctggctgc ccagctccac cccgagccgg aagctgatct gggcgatgcc
     6241 ggcggcattg ctctgcaccg tggcggagga gagcgctccc acccccagct ttcctgagcc
     6301 cttgaccacc ttgaagagca cctcctggcc ggccacagcc tgccccgcct tgtcgacaat
     6361 tttcaccgcc aggggaaacg gtagcacctc gccggcctgg gcgctggcgc cgccgccgct
     6421 gacaccatcc atcttgtggg cgaggctctt gcgggcgctc acgtaaaacc tgaccgggga
     6481 gccggagaga tggactccgc tgccctgaaa ggactgggcc tcaaagacat tgttggcgat
     6541 gccataggcg gagcccaggg tggccgtgac cgccaccacc ccgttggcat cgctgatcct
     6601 ggtgacctca ggctgtcctt ccagatggcc gccgccgcgc cgcacccaga aggtcaccgg
     6661 atggttgggc acggcatagc cgctgagg
//
LOCUS       DAOPWU010000183         6603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_11576_length_6603_cov_3.704795, whole genome shotgun sequence.
ACCESSION   DAOPWU010000183 DAOPWU010000000
VERSION     DAOPWU010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11576_length_6603_cov_3.704795"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..779
                     /locus_tag="PKM23_09790"
     CDS             30..779
                     /locus_tag="PKM23_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY91805.1"
                     /translation="MNNSFSFSWPEGYRPGAEVWARIRFSSSRQLSAVQNPAGISDSA
                     GEVQDYRFDLAPVVLGLFEARPANEAIRVEWQTQAENDNLGFHLFRARAEEGPYSQVT
                     RNLIHGAGNASSRRSYEYSDATVEPGRIYYYKLADVDYRGRMTMHGPVCVTATAPVDY
                     VLEQIYPNPFNPEARINFRLKEAGRVLLSVFDLRGREVRRLMAAALPGGTHTISWNGK
                     DDNGLSMSSDTYIYKLQVNDYEISRRIEFVR"
     gene            981..2516
                     /locus_tag="PKM23_09795"
     CDS             981..2516
                     /locus_tag="PKM23_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNY91806.1"
                     /translation="MTLYPRKYPVLPFLSLCALLLAGGCAHQRSGTLTVLHTNDQHSQ
                     FAAAPATWVQKDPKPRIGGLVALENQIRKARENYKATLLLDAGDFMTGTPIAKLKVEG
                     ALGGAYVQMLNDMGYQALTLGNHEFDEGQENLMGLVRLARYDILSANLYKDDRLFAPK
                     SHAIYEIGGLRVGLIGLTLTDLFDMTARKNLEGIRVLDPAASAQKIIDEIDGRTDLII
                     LLTHMGVEADRDLAKKIRGADIIVGGHSHSRLNKPIIENGVLIVQADSKTRYLGRLTV
                     DVAGDAVVRHDYALIPCWVDSVTQPDPLLAGRIKEYQQQIDADYGRTIGQLTTDWQRN
                     SQGESNIGNYIADVMRLTAAADFAVINSGGIRKDMPAGPIKKLDIVEILPFTNTLVTF
                     TCTGEELMKIIQSNVRSAAREEPGVLQVSGLKYQYRVTPGGTIEIASAMINGNAIDPQ
                     ATYTGATIDFILFGQAERYFGFAPTGKTENTNQLLSDVVMTQIETQPAVASSIEGRMV
                     RIP"
     gene            2598..4043
                     /locus_tag="PKM23_09800"
     CDS             2598..4043
                     /locus_tag="PKM23_09800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNY91807.1"
                     /translation="MKRHASWIAVVAALLLIGWLIACAINPVTGKRQLMLLSESDEVA
                     LGQQTDAEVIATYGLYDNAALTSYVNGVGQKMTKISHRPNLAYSFKVLDTDVVNAFAV
                     PGGYIYVTRGILAYLNDEAELAGVLGHELGHVAARHTADNYTKQQLAQIGLTLGSVLS
                     EKFRQYANIASFGVSMLFLKFSRDDERQADDLGVEYSSKALYDANRMATFFETLERMH
                     EGSSSSGLPEWFSTHPNPENRIGAVRNKTREWQAKLAGNSFMVNRPQYLASIDGLVFG
                     EDPRQGYVEANTFYHPSLKFQFAVPQGWTVNNTPAQVQMVSPNQDANLLFGLQGSAKA
                     AAVANQFVTESSAAVMRSDAININTFPTQRVQSRIISQTDTLGVLSYFIQKGNDVYIF
                     HGISAPNALSTWYNTFANAVGRFNNLTDAKRINVAPKVLKVQNGKNSTTLSTLLAANG
                     VPSTDLEKFAVVNGMKLNDLVPASTKYKIAK"
     gene            complement(4124..>6603)
                     /locus_tag="PKM23_09805"
     CDS             complement(4124..>6603)
                     /locus_tag="PKM23_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNY91808.1"
                     /translation="NFSSRVQMVIQFARDEALRLGHDYIGTEHLLLGLIREGEGVAVE
                     ILHALGCDLEEIRAALEEAGRIAGDTMTVGNIPFTKRAEKILKNAYMEADHNKSDIIG
                     TEHLLLALVKEKEGLAAQILKSFGVTYEAVTEELERILQGSANAAAQSESQQQKSQTP
                     ALDHFGRDLTELARRGELDPIIGREKEIQRVAQILSRRKKNNPVLIGEPGVGKTAIAE
                     GLALRIIEKKVPRILHNKRVITLDLGAIVAGTKYRGQFEERMKAILNELVKTRDVILF
                     IDELHTIVGAGGASGSLDASNMFKPALSRGELQCIGATTLDEFRQYIEKDGALERRFQ
                     KVIVDPPSLDETFAIVKGLQSRYEAHHRVRYTEEAIRDIVKLSDRYITDRFQPDKAID
                     VLDETGARVHLANIVVPRDISDLEEQVKRVRAEKDQVVRSQNFEKAAVLRDREKQLLR
                     DLEASKKRWNDEQDSAYAKVSEEDVSEVVSMITGIPVQRVAQSESDRLLTMGTALKKR
                     IVGQDPVIDILSRAIQRTRAGLKDPNRPIGSFIFLGPTGVGKTQLAKELAAYLFESED
                     ALIRIDMSEFMEKFSVSRLTGAPPGYVGYEEGGQLTEKVRHHPYSVVLFDEIEKAHPD
                     VFNILLQILDEGHLTDSLGRKVDFRNTVLILTSNIGARQISRGGSLGFNKSDEETDYE
                     SMRERLIEEVRHVFNPEFLNRVDDIVVFRQLNRGDMLAIVDIVIAEMLGKVADRNISI
                     ELTKGAKEFIAERGFDPMNGARPLKRTIQRHVEDAIAEELLKGHFGDGSIIRERKKGD
                     GLDFIDARELAGSVEGDENQGTQALEP"
BASE COUNT         1287 a         1989 c         1987 g         1340 t
ORIGIN      
        1 attggcattg ttggtatatg tgttaacaca tgaacaacag cttcagtttt tcctggccgg
       61 aaggataccg ccccggtgcg gaggtctggg cgcgtattcg tttcagcagc agccggcagc
      121 tcagcgccgt gcagaacccg gccggcatca gtgattcagc cggagaggtt caggactatc
      181 gtttcgatct cgccccggtc gtcctcgggc tctttgaagc ccggccggcg aatgaggcga
      241 tcagagttga gtggcagacc caggcggaga acgataatct gggcttccat ctctttcgtg
      301 cccgtgcgga ggaggggcct tacagccagg tcaccagaaa tctgatccat ggcgctggga
      361 atgccagctc acgccgctcc tatgagtaca gcgacgcgac ggtggaaccg ggcaggatct
      421 actactacaa actggcggat gtggactatc gggggcgcat gaccatgcac ggtcccgtct
      481 gcgtaacagc cacagcgccg gtcgactatg tcctcgagca gatctatccc aatcccttca
      541 atcccgaagc gcggataaat ttccggctca aggaggcagg gagggtcctg ctctccgttt
      601 ttgatctgcg cggccgcgag gtgcgcaggc tgatggccgc tgccctgccg ggcggcaccc
      661 ataccatctc ctggaacggc aaggacgaca acggtctttc catgtcttcg gatacctata
      721 tctacaagct gcaggtcaac gattacgaga tatcccgccg catcgaattc gtccgctgat
      781 gcccggcccc gggctcgccc cgcagggggc acaccagaga aaccatccaa aaccgccgcc
      841 gcgagtaggc ggtttttttt atgcttcccc ccggcgcaat aaaatacttg acaaacgcgg
      901 cacaaatcgc taaaatggta taaattttgt cgggcagccc ggcatggccg ggctgcgcta
      961 cccaaactca agaggacttt gtgaccctgt acccccgaaa atacccggtt ctgccgtttc
     1021 tatccctctg cgccctcttg ctcgccggcg gctgcgcgca tcagcgcagc ggaaccctga
     1081 ccgtcctgca caccaatgac cagcacagcc agttcgccgc cgctccggcc acctgggtgc
     1141 agaaagatcc caaaccccgg atcggcggcc tcgtcgccct cgaaaaccag atccgcaagg
     1201 cccgggagaa ctacaaggcg actcttctgc tcgatgccgg cgatttcatg accggcaccc
     1261 cgatcgccaa gctcaaggtg gagggcgcac tcggcggagc ctatgtccag atgctcaacg
     1321 acatgggata ccaggccctc accctcggca accacgaatt cgacgagggg caggagaatc
     1381 tcatggggct cgtccgtctg gcgcgctatg atatcctctc cgccaatctc tacaaggatg
     1441 accgcctctt tgccccgaaa tcgcatgcca tctacgagat cggcggactg cgggtcggct
     1501 tgatcggcct caccctgacc gacctcttcg atatgaccgc caggaagaac ctcgagggta
     1561 tccgcgtcct cgatccggcc gcatccgccc agaagatcat cgacgagatc gatggcagga
     1621 ccgaccttat catcctgctc acgcatatgg gggtggaggc cgaccgcgac ctggccaaaa
     1681 agatccgcgg ggccgacatc atcgtcggcg gccacagcca ctcccggctc aacaagccga
     1741 tcatcgagaa cggggtgctc atcgtccagg ccgattccaa gacccggtac ctgggccggc
     1801 tcacggtcga tgtcgccgga gatgccgtcg tgcgccatga ctacgccctc atcccctgct
     1861 gggtggacag cgtcacccag cccgatcccc tcctcgccgg acggatcaag gagtaccagc
     1921 agcagatcga cgccgattac ggccggacca tcggccagtt gacaaccgac tggcagcgta
     1981 acagccaggg agagtccaat atcggcaatt acatcgccga tgtcatgcgt ctgacggcgg
     2041 cagctgactt cgctgtcatc aacagcgggg gcatccgcaa ggatatgccc gccggcccga
     2101 tcaaaaagct ggatattgtc gagatcctcc ccttcaccaa taccctggtc accttcacct
     2161 gcaccggtga agagctgatg aagattatcc agagcaacgt gcgctcggcg gccagggaag
     2221 agccgggtgt gctgcaggtc tcgggtttga agtatcagta ccgggtcacg ccgggcggga
     2281 ccatcgagat cgccagcgcc atgatcaacg gcaacgctat cgatccgcag gccacctaca
     2341 cgggggcgac gatcgatttc atcctcttcg ggcaggcgga gcgctatttc ggtttcgccc
     2401 cgacgggaaa aacggagaac accaatcagc tcctctccga cgtggtcatg acgcagatcg
     2461 aaacccagcc agccgtcgca tcatccatcg agggccggat ggtacgtata ccctgatcgt
     2521 ggcacgctcg gtgcggggga gcccgctgca gcgggcaagg tggctgcagg tagtaataaa
     2581 atgtacaggg aggtaaaatg aaacgccatg caagctggat tgccgtcgta gcagcactgc
     2641 tgctgatcgg ctggctgatc gcctgtgcca tcaatccggt gaccggaaag cgtcagctta
     2701 tgctgctctc cgagagcgac gaggtcgccc tcggtcagca aaccgacgcc gaggtcatcg
     2761 ccacctatgg cctctatgac aatgccgcct tgacatccta cgtcaatggc gttggtcaga
     2821 agatgaccaa gatatcccac cgcccgaatc tggcctattc gttcaaggtc ctggataccg
     2881 atgtggtcaa tgccttcgct gtgccgggcg gctacatcta tgttacgcgc ggcattcttg
     2941 cctatctcaa cgacgaggct gagctggccg gtgttctcgg ccacgagctg ggtcatgtcg
     3001 ccgcgcgcca taccgcggat aactatacca agcagcagct ggcccagatc gggctcacgc
     3061 tcggttcggt gctctcggaa aagttccggc agtacgccaa tatcgccagt ttcggcgtca
     3121 gcatgctctt tctcaaattc agccgtgatg atgagcggca ggcggacgac ctgggcgtcg
     3181 agtactcgag caaagccctc tacgacgcca ataggatggc cacttttttc gagacccttg
     3241 agcgcatgca cgaggggagc tccagcagcg gtctgccgga gtggttctct acccacccca
     3301 atcccgagaa tcgcatcggg gcggtgagga acaagaccag ggaatggcag gccaaactcg
     3361 cgggcaacag ctttatggtc aaccgtcccc aatatctggc gagcatcgac ggcctggtct
     3421 tcggcgagga tccgcgccag ggctacgttg aggccaacac cttttaccat ccctcgctca
     3481 aattccagtt tgcggtaccg cagggctgga cagtcaataa tactccggcg caggtgcaga
     3541 tggtctcccc caaccaggat gccaatctgc tcttcggcct gcagggctcc gccaaagccg
     3601 ctgccgtcgc caatcaattc gtcaccgaat cctccgccgc ggtgatgcgt tccgacgcca
     3661 tcaacatcaa cacctttccg acacagcggg tgcagagccg gatcatctcg cagactgaca
     3721 ccctgggtgt gctctcctac ttcatccaga aggggaacga cgtttatatc ttccacggga
     3781 tttcggctcc caatgctctc tccacctggt acaacacctt tgcgaacgcg gtcgggcggt
     3841 ttaacaacct gactgacgcg aagaggatca acgttgcgcc caaggtactc aaggttcaga
     3901 acggcaagaa ttcgacgacc ctttcgaccc ttctggcggc caatggcgtt ccctcgaccg
     3961 atctggaaaa gttcgcagtg gtcaacggga tgaagctcaa tgatctggtc ccggccagca
     4021 ccaaatacaa gatagccaaa tagtcccggc tgctccacgg caataaaaaa agccctcttt
     4081 cgcaagaaag agggcttttt ttatggaaag gagtggcgcg ggatcagggc tcgagcgcct
     4141 gggtgccctg gttttcgtcg ccttcgacgc tcccggcgag ttcgcgggca tcgataaaat
     4201 cgaggccgtc gcctttcttg cgctcgcgga tgatactgcc gtcgccgaaa tggcccttga
     4261 gcagttcttc ggcgatggca tcctcgacgt ggcgctgaat ggtccgtttg agcggcctgg
     4321 cgccgttcat cggatcgaag ccgcgctccg cgatgaactc cttggcccct ttggtcagct
     4381 cgatgctgat gttgcggtcc gcgactttgc cgagcatctc ggcgatgacg atgtcgacga
     4441 tggcgagcat gtcaccgcgg ttgagctgcc ggaagacgac gatgtcgtcg acgcgattaa
     4501 ggaattcagg attgaagaca tgccggacct cctcgatcag gcgctcccgc atcgattcat
     4561 aatcggtctc ctcgtcgctc ttgttgaagc cgagggagcc gcccctgctg atctggcggg
     4621 cgccgatgtt gctggtcagg atcagcacgg tgttgcggaa atcgaccttg cgcccgaggc
     4681 tgtcggtgag atggccttca tccaggatct ggagcaggat gttaaagaca tcggggtgcg
     4741 ccttttcgat ttcgtcaaag aggaccaccg aatagggatg gtggcgcacc ttttcggtga
     4801 gctgcccgcc ctcttcataa ccgacatagc ccggaggcgc gccggtgagc cgggatacgg
     4861 agaacttttc catgaactcc gacatgtcga tacggatcag ggcatcctcg ctctcgaaga
     4921 ggtaggcggc gagttccttg gcgagttggg tcttgccgac cccggtcggt cctaaaaaga
     4981 tgaaggagcc gatggggcgg tttggatcct tgagcccggc gcgggtgcgc tggatggcgc
     5041 ggctgaggat gtcgatgacc ggatcctggc cgacgatccg cttcttgagg gcggtaccca
     5101 tggtgagcag ccggtcggat tcggattggg ccacgcgttg caccggaatg ccggttatca
     5161 tcgaaaccac ttcggagaca tcctcttcac tcactttagc gtaggcggag tcctgttcgt
     5221 cgttccagcg tttcttgctc gcctcgagat cgcgcaggag ctgtttctcg cggtcgcgca
     5281 ggacggccgc cttttcgaaa ttctgggagc gcaccacctg gtccttttcg gcgcggacgc
     5341 gtttgacctg ctcttccaga tcgctgatgt cgcgcgggac gacgatattg gcaaggtgga
     5401 cgcgggcgcc ggtctcatcg aggacatcga tggccttgtc gggctgaaag cggtcggtga
     5461 tgtaacgatc cgagagcttg acgatatcgc gaatcgcctc ttcggtatag cggacgcggt
     5521 gatgcgcctc atagcggctc tgcagcccct tgacgatggc gaaagtctca tcgaggctgg
     5581 ggggatcgac gatcactttc tggaagcggc gttcgagggc gccgtctttc tcgatgtact
     5641 ggcggaactc gtcgagggtg gtggcgccga tgcactgcag ttcgccgcgc gatagggccg
     5701 gcttgaacat attggaggcg tcgagggagc cggaagcccc gcccgcgccg acgatggtat
     5761 ggagctcatc gatgaagagg atgacgtcgc gggtcttgac cagctcgttc aggatcgcct
     5821 tcatgcgttc ttcgaactgg ccgcggtact tggtgccggc gacgatcgcc ccgagatcga
     5881 gggtgatgac gcgcttgtta tgaaggatgc gcggcacctt tttttcgatg atgcgcagcg
     5941 ccagcccctc ggcaatggcg gttttgccga cgccgggttc gccgatgagg acgggattgt
     6001 tcttcttgcg gcggctgagg atctgggcga cgcgctggat ctctttttca cggccgatga
     6061 tcggatcgag ctcgccgcgg cgtgccagtt cggtcaggtc gcggccgaag tggtcgaggg
     6121 ccggagtctg ggatttctgc tgctgggact cgctctgggc tgcggcattg gccgatccct
     6181 ggaggatgcg ctcgagctcc tcggtcaccg cttcataggt cacgccgaag cttttgagga
     6241 tctgcgcggc cagtccctct ttctccttga ccagggcgag gaggaggtgt tcggtgccga
     6301 tgatgtcgga tttgttatgg tccgcctcca tataggcgtt cttgaggatt ttttcggcgc
     6361 gcttggtgaa ggggatgttg ccgacggtca tggtgtcgcc ggcgatccgt ccggcctctt
     6421 cgagggcggc gcggatctct tcgaggtcgc agccgagggc atgcaggatt tcgacggcga
     6481 cgccctcgcc ttcacggatc agcccaagca gcaggtgctc cgtgccgata tagtcatgtc
     6541 caagtcggag tgcttcatct cgtgcaaact ggatgaccat ttgtacgcga cttgaaaagt
     6601 tat
//
LOCUS       DAOPWU010000184         6515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_11775_length_6515_cov_5.913467, whole genome shotgun sequence.
ACCESSION   DAOPWU010000184 DAOPWU010000000
VERSION     DAOPWU010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11775_length_6515_cov_5.913467"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..2364
                     /locus_tag="PKM23_09810"
     CDS             160..2364
                     /locus_tag="PKM23_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNY91809.1"
                     /translation="MSGHSNSSTRKQVVIQVGSNHKIDYLPAEHAARYYGVNSFSDQV
                     MREKLPTEVYKKLRATIRKGEKLDMTIADAVAHAMKEWALAKGVTHFTHWFQPQTGST
                     AEKHAAFIEIEEDGSVIERFRGEQLVQGEPDASSFPSGGMRSTFEARGYTMWDPSSPA
                     FIMEGPRGGILCIPSVFISYTGEALDKKTPLLRSMEAINRSAQKVLALFGAQSAGRVV
                     TAIGTEQEYFLIDKAFYNLRPDLRITGRTLLGARPPKGQQLEDHYFGSIKERVLAFMQ
                     DAETELYKVGVPAKTRHNEVAPHQFEIVPIYAEANVGADRNHLIMEILKRVANRHDLA
                     LLLHEKPFAGINGSGKHNNWSISDAEGNNLLDPGKTPEENLQFLVFLTAVLRAVYFYG
                     DLLRASIASAGNDHRLGANEAPPAIMSVFLGDQLTAILDAIKAGKAARATNESIIDLG
                     IGQLPKMLRDYTDRNRTSPFAFTGNKFEFRAIGSSASVSMANTVLSAAVADSLDVLSA
                     EIEKETGKGAELKVAVLSVLRKHIKETEPIRFNGNNYSKEWEQEAARRNLPNIRNSAY
                     ALDALVAPKNIEMLVRQRTFTRTELEARYHTKLEQFIKALQIEAETLIHMTDTLVIPA
                     AIGYQERLVKTVRGLKTLQEELGGVVFASEVELLKRITELIEQCGQKTRALKAAMVRA
                     EGMEDLSKCAKFVADHIRPLMEELRKPVDELEVTIDDESWPLPRYSEMLFIL"
     gene            complement(2428..2580)
                     /locus_tag="PKM23_09815"
     CDS             complement(2428..2580)
                     /locus_tag="PKM23_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HNY91810.1"
                     /translation="MADTAYCVKCKAKTEVKDGKKVTMKNGRAALKGTCAKCGTGVYK
                     ILGKAK"
     gene            complement(2709..3134)
                     /locus_tag="PKM23_09820"
     CDS             complement(2709..3134)
                     /locus_tag="PKM23_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNY91811.1"
                     /translation="MKGALREKIYWQLPAWLWGFFIFALTSLPKLTPPSLGFELEDKV
                     YHFIVYAVLGLLIARAAVQGGREPLRSGIRRMLWIGIPLSFIDEVHQAFIPGRFCDGL
                     DALADILGLLLAAIIFVLWGSRLAEKDRSIYCNLVKCDT"
     gene            complement(3131..4672)
                     /locus_tag="PKM23_09825"
     CDS             complement(3131..4672)
                     /locus_tag="PKM23_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNY91812.1"
                     /translation="MQIIVTAAEMAALDRDCQQLLQMPSLLLMENAGRGIAAVAKTML
                     GEPEGKRVLIVSGPGNNGGDGYVVARHLANAGCRVTLWVLAARDKIRGDALTNLVIWE
                     KMGGQTLFIDAIPDAPAPLPHLIVDALLGTGAAPPLSTPFAMMATLLNELGAPILAVD
                     IPTGIDADTGAVPGPAIRARATAVMARLKRGLLFSPGREQAGEIHLIDISMPPEWAPK
                     SGTPVRRLEAADIRARLPRRPADAHKFSIGSVGVIAGSIGYTGAACLTAAAALRAGCG
                     LSCLAAAKSLLPLLTGKLPEVITWPFEDEESGCLREAALPQWLSPLAAQNALAVGPGL
                     GRHPDTGGLVRRLLREMKQPMVLDADGLNLCTDYLDLLQNYQGALILTPHPGELSRLT
                     GLTTAKILTRPVEVAAEWAVKLGQLLVLKGGPTVIAAPDGSVFVNSTGNPGMATAGAG
                     DILTGLIAGLLAQGLAPIDAALCGVWLHGAAGDRARERRGEMGMIAGDLLEALPDALR
                     GVLHP"
     gene            complement(4743..5117)
                     /gene="acpS"
                     /locus_tag="PKM23_09830"
     CDS             complement(4743..5117)
                     /gene="acpS"
                     /locus_tag="PKM23_09830"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNY91813.1"
                     /translation="MVHAIGTDIVDLDRFASAVERWGDHFLGRILTPDEIAYCRRKAS
                     GIESMAARFAAKEAFIKCLSEEEYRHFRWHDVRVENAANGRPRLVLSGSLARILPDLQ
                     ILLTLSHSRLSAVAVVILQSRP"
     gene            5294..5875
                     /locus_tag="PKM23_09835"
     CDS             5294..5875
                     /locus_tag="PKM23_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNY91814.1"
                     /translation="MDPISAQLVRDAIEGDQKAYAEIVHRYRNQIYNLILRMVRRREE
                     AEDLTQETFIKAFNALSSFNAEYAFSTWLYKIAVNNCIDHFRKKRLKTYPLDNPIPAR
                     DGELQREFPDHEAGPDAGLMEKERHLTIQEAIDSLPGKYREAILLRHAQDRSYEEIAR
                     LLGIPIGTVKVRIFRAREMLKKKLRDQRKNLFS"
     gene            complement(5885..>6515)
                     /locus_tag="PKM23_09840"
     CDS             complement(5885..>6515)
                     /locus_tag="PKM23_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HNY91815.1"
                     /translation="LSLYGLTIEAGTPLAQAVAGGRLHPCDEELEREMFLAGKRMAEA
                     AGYQHYEISNYARPGCEARHNQHYWAGAPYLGLGPAAHSFAGGERFWNGRDLEGYIDA
                     WEQDEPAVAGRERIGREEEKSEALLLGLRRRRGIDLELWRERFGEELLERGSRTIARL
                     GGLLHGHPSFSASPGAELLTLNGQELALTEAGVLLYDMVCQELCAGLCA"
BASE COUNT         1271 a         2075 c         2010 g         1159 t
ORIGIN      
        1 atcataataa acaatggttt gggggctgaa aatggcggcc gctcccgccc cgttttgctc
       61 ttcgtggcac tgcttttgca aatgggcgcc caataagccg aatcagcacc agtgcggctt
      121 ctcatccatc catcccattc tctcagaagg agcgtcacca tgtccggtca ttctaactct
      181 tccacgcgca aacaggtcgt gatccaggtc gggagcaacc acaagatcga ctacctcccc
      241 gccgagcatg ccgcccgcta ttacggcgtc aacagcttca gcgaccaggt gatgcgcgaa
      301 aagctgccca cggaggtcta caagaagctg cgcgccacca tccgcaaggg ggagaaactc
      361 gacatgacca tcgccgatgc cgtcgcccat gccatgaagg agtgggccct ggccaagggg
      421 gtgacccatt tcacccactg gttccagccc cagaccggct cgacagcgga gaagcacgcc
      481 gccttcatcg agatcgagga ggatggcagc gtcatcgagc gcttccgcgg cgaacagctg
      541 gtccagggcg aaccggacgc ttcctccttt cccagcggcg ggatgcgctc gacctttgag
      601 gcgcgcggct atacgatgtg ggatccctcg agtccggcct tcatcatgga ggggccccgc
      661 ggaggcatcc tctgcattcc ctcggtcttt atcagctata ccggcgaggc cctcgacaaa
      721 aagacccccc tgctgcgctc gatggaggcc atcaaccgct ccgcgcaaaa ggtgctcgcc
      781 ctctttggag cccagagcgc cggacgggta gtcaccgcca tcggcacgga gcaggagtat
      841 tttctcatcg acaaggcctt ttacaatctt cgtcctgatc tgcgcatcac cggccgcacc
      901 cttctcggcg cccggccgcc caaggggcag cagctcgagg accactactt tggctcgatc
      961 aaggagcggg ttctcgcctt catgcaggat gccgagaccg aactctacaa ggtcggggtc
     1021 ccggccaaga cccgccacaa cgaggtggca ccccaccagt tcgagatcgt acccatctac
     1081 gcggaagcga atgtcggggc ggaccgcaat catctcatta tggagatcct caaacgggtc
     1141 gccaaccggc atgatcttgc tctgctcctg catgaaaaac cctttgccgg catcaacggc
     1201 agtggaaaac acaacaactg gtcgatcagc gatgccgagg gcaacaacct gctcgatccc
     1261 ggcaagaccc cggaagagaa tctgcagttt ctggtctttc tgacggcggt tctgcgtgcg
     1321 gtctattttt acggcgacct gctgcgcgca tcgatcgcct cggcgggcaa cgaccatcgc
     1381 ctcggcgcca acgaggcccc accggcgatc atgtcggtct ttctcggcga tcagctcacc
     1441 gccatcctcg atgccatcaa ggccggcaag gcggccagag cgaccaatga gagtatcatc
     1501 gatctcggca tcggccagct gcccaagatg ctgcgcgatt acaccgaccg caatcgcacc
     1561 tcccccttcg ccttcaccgg caacaaattc gagttccgcg ccatcggctc ctccgcctcg
     1621 gtctccatgg ccaataccgt actcagcgcg gcggtcgccg attcccttga tgtgctgagc
     1681 gccgagattg aaaaggagac cggcaagggg gccgaactca aggtggcggt gctgtccgtt
     1741 ttgcgcaagc acatcaagga gaccgagccg atccgcttca acggcaacaa ctacagcaag
     1801 gagtgggagc aggaggcggc cagacgcaac ctgcccaaca tccgcaactc cgcctacgcc
     1861 ctcgatgccc tggtggcgcc gaagaacatc gagatgctgg tgcgtcagcg caccttcacc
     1921 cgcactgaac tcgaggcccg ttatcatacc aagctcgagc agttcatcaa ggccctccag
     1981 atcgaggccg aaaccctcat ccatatgaca gacaccctgg ttatcccggc ggccatcggc
     2041 tatcaggagc gcctggtcaa gaccgtgcgg ggactgaaga ctctgcagga ggaactggga
     2101 ggggttgttt ttgccagcga ggtcgagctg ctcaagcgca tcaccgaact catcgagcag
     2161 tgcggacaga agaccagggc attgaaggcg gcgatggtgc gcgcagaggg gatggaggat
     2221 ttgtcgaaat gcgccaagtt tgtggccgac cacatccggc cgctcatgga ggagctgcgc
     2281 aagccggtcg acgagctcga ggtgaccatc gatgacgaga gctggccgct gccgcgctat
     2341 tcggagatgc tcttcattct ctaggcgggg ggcaaaaaaa atgggcggtg agtcaactca
     2401 ccgcccatat acgcattttt atggtggtta tttggccttg ccaagtatct tgtagacgcc
     2461 ggtgccgcat ttggcgcagg tgccttttag agctgcacga ccatttttca tggtcacttt
     2521 tttgccatct ttgacctcgg tcttggcttt gcacttgaca cagtatgcag tatccgccac
     2581 gtcgagttcc tcccgttaaa ttaatcccca cggtacatta acttacgctt aaattaacgc
     2641 attcgcaggc aaaagtcaat taaaagttcg tatgcgttga aaaaagtgga ctctctccgg
     2701 ctagccccct atgtgtcgca ttttactaaa ttacaataaa ttgagcgatc cttttccgcc
     2761 aaacggctgc cccacagcac aaaaataatt gcggccagca gcaatccgag gatgtcggcg
     2821 agggcgtcca ggccgtcgca gaagcggccc gggatgaagg cctggtgcac ctcgtcaata
     2881 aaggagaggg ggataccaat ccagagcatg cgcctgatgc cggagcggag gggctcccgc
     2941 ccgccctgga cggcggcccg ggcgatgagg aggccgagca cggcatagac gatgaaatgg
     3001 tagaccttgt cttccagctc gaagccgagg gaaggcgggg tcaatttggg cagcgaggtg
     3061 agggcgaaga tgaagaaacc ccagagccag gccggcagct gccagtagat tttttcccgc
     3121 agcgcgcctt tcacggatgg aggacccccc ggagggcgtc gggcagggcc tcgagcagat
     3181 ccccggcgat catgcccatc tctccgcggc gctcccgcgc ccggtcgccc gcggcgccgt
     3241 gcagccagac cccgcagagg gcggcgtcga tgggcgcaag gccctgggcg aggagtccgg
     3301 cgatcaggcc ggtgaggata tcgccggcgc cggcggtagc catgccggga ttgccggtgc
     3361 tgttgacaaa gaccgagccg tccggcgcag cgatgacggt ggggccgccc ttgaggacga
     3421 ggagctggcc aagcttgacg gcccactcgg cggcgacctc caccggccgg gtgaggatct
     3481 tcgcggtggt cagtccggtc agccgcgaga gctcgcccgg gtggggggtg aggatcagcg
     3541 cgccctgata gttttgcagg aggtcgagat aatcggtgca gaggttgagc ccgtcggcgt
     3601 cgagcaccat gggctgcttc atctcgcgca ggaggcggcg gaccagcccg ccggtgtcgg
     3661 gatggcggcc gaggccgggt ccgacggcga gggcgttctg ggcggcgagg gggctcagcc
     3721 actgcggcag cgcagcctcg cgaaggcagc cgctttcctc gtcttcaaaa ggccaggtga
     3781 tgacttcggg taatttgcct gtgagcaggg gcagcaggct tttggcggcc gccaggcagg
     3841 agaggccaca gccggcgcgc agggcggccg cagcggtcag gcaagccgcg ccggtgtagc
     3901 cgatggaacc ggcgatgacc ccgacgctgc cgatgctgaa tttatgggca tcggccgggc
     3961 ggcggggcag tctggcgcgg atgtcggcgg cctcgaggcg gcgcaccggg gtgccgcttt
     4021 taggggccca ctccggcggc atgctgatgt cgatgaggtg gatttccccg gcctgctcgc
     4081 gccccggcga gaagagcagc ccccgtttca gccgggccat cacggccgtg gcccgggccc
     4141 tgatcgccgg ccccggcacc gccccggtat cggcgtcgat tccggtgggg atgtcgaccg
     4201 cgaggatggg cgcccccagc tcattgagca gggtggccat catcgcaaag ggggtgctca
     4261 ggggaggggc cgcgccggtg ccgagcaggg catcgacgat cagatgcggc aggggcgcgg
     4321 gcgcatcagg gatggcatcg ataaaaagag tctggccgcc catcttctcc cagatgacca
     4381 gattggtcag ggcatccccg cggatcttgt cgcgggcggc gagaacccag agggtcaccc
     4441 ggcagccggc attggccaga tggcgagcca ccacatagcc gtcgcctccg ttgttgcccg
     4501 gcccgctgac gatgagcacc cgcttgccct ccggctcgcc gagcatggtt ttggccacgg
     4561 cggcgatccc gcgcccggcg ttctccatga gcagaagtga gggcatctgc aggagctgtt
     4621 gacaatcccg atcgagtgcg gccatctcgg cggccgtgac gattatctgc atctctatct
     4681 ccggatatct ccagaatcag aacattcact gtttgaaccc tccggctcac cccgggacgc
     4741 gctcacggcc tggactgcag gatcaccacc gcgacggcgc tgaggcggct gtggctgagg
     4801 gtgagcagga tctgcaggtc gggcaggatg cgggcgaggc ttccgctcag caccagtcgg
     4861 ggcctgccgt tggccgcatt ctcgacccgc acatcgtgcc agcggaagtg gcgatactcc
     4921 tcctcgctca ggcacttgat aaaggcctcc ttggcggcga aacgggcggc catcgattcg
     4981 atgccggagg ccttgcggcg gcagtaggcg atctcgtcgg gggtgagaat gcggccgaga
     5041 aaatggtcgc cccagcgctc gactgcagag gcgaaccggt cgagatcaac gatgtcggtg
     5101 ccgatggcgt ggaccatttt tccatccctc ccagccggca gcggggcaac tgcctacgat
     5161 ccgccggaac tatttaaaaa ttaacatata tggccatcta atgcaataac ttttccttgc
     5221 ttgtcgatct tttttactgt accttattta tcaaagccca tttgattttt caccggacga
     5281 gtgcccgatc atcatggatc cgatttccgc acaacttgtt cgcgatgcca tcgaggggga
     5341 tcagaaagcc tacgccgaga tcgtccaccg ctatcgcaac cagatctaca atctcatcct
     5401 gcggatggtc cggcggcgcg aggaggcgga ggacctcacc caggagacct tcatcaaggc
     5461 cttcaacgcc ctctccagtt tcaacgccga atatgccttt agcacttggc tctacaagat
     5521 cgctgtcaac aactgcatcg accatttccg caagaaacgc ctcaagacct accccctgga
     5581 caatccgatc ccggcccggg atggcgaact tcagcgcgaa ttccccgatc acgaagccgg
     5641 ccccgacgct ggcctgatgg agaaggagcg ccatctcacc atccaggagg ccattgactc
     5701 tttgcccggg aaatatcgcg aggcgatcct gctccgccac gctcaggacc gatcctatga
     5761 agagatcgcc cgcctcctgg gcatccccat cggcaccgtc aaggtccgca tcttccgcgc
     5821 ccgtgagatg ctgaagaaaa aattgcgcga tcagcgcaaa aatctcttca gctgatcccg
     5881 cgcttcacgc gcacaagcct gcgcagagct cctgacagac catatcgtag agcagcaccc
     5941 ccgcctcggt gagggcgagc tcttgtccgt tgagggtgag cagttccgcc ccgggtgaag
     6001 cgctgaaaga ggggtggccg tgcaacaggc cgcccagcct ggcgatggtg cggctcccgc
     6061 gctcgagcag ctcctcgccg aagcgctccc gccaaagttc gaggtcaatt cccctccgcc
     6121 tgcgcagacc gagcaggagg gcctcgctct tctcctcctc cctcccgatc cgctcgcggc
     6181 cggcgacagc cggctcatcc tgctcccatg catcgatata accctcgagg tcgcggccgt
     6241 tccagaagcg ttcgccgccg gcgaaggagt gggcggccgg gccgaggccg agatagggcg
     6301 cccccgccca atagtgctgg ttatggcgcg cctcgcaccc tggccgggcg taattggaga
     6361 tctcgtaatg ctgatagccc gccgcctcgg ccatcctctt gcccgcgagg aacatctccc
     6421 gttcgagctc ttcatcgcag gggtggagcc ggccgccggc gaccgcctgc gcgagggggg
     6481 tgccggcttc gatggtgagc ccgtagaggg agaga
//
LOCUS       DAOPWU010000185         6494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_11820_length_6494_cov_4.485945, whole genome shotgun sequence.
ACCESSION   DAOPWU010000185 DAOPWU010000000
VERSION     DAOPWU010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11820_length_6494_cov_4.485945"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PKM23_09845"
     CDS             complement(<1..997)
                     /locus_tag="PKM23_09845"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNY91816.1"
                     /translation="MKKLTLLLCLFLVTGLAVVRAQAPYGVGDNIDVPYVAPGTLTLD
                     GNGNESAWNSAVEVNMIGFWDGAWGGVPFPDIEAKAKLLWSEDWLYIYAKVEDYEFFF
                     GPDTANWIGEQIMVGVDRILAGDPPGGNYADDWSGAPWNAPDKGPTVYKINDKGITLN
                     WGGWWDDPNQTWWELAPIDSGWVAGQVFVNNDDLTWGVEMGIWLPQIRTNSMVGFNIG
                     GAAATPDTTAVPWEWGEGTYAYYAWHPQPADTAVGYPSDYIAGDIQRRAQSFGTLTFV
                     GGPEPYGIADNIDVPWVAPDAIVFDGVGDEAAWTNAAAVNMIGFWDGAWGGVPFPD"
     gene            complement(1174..2016)
                     /locus_tag="PKM23_09850"
     CDS             complement(1174..2016)
                     /locus_tag="PKM23_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY91817.1"
                     /translation="MKPTARTILHISKRTLFYLFIYGAALATLAPFIWMVLTSLKDLN
                     EIFVYPPRWLPKTFHFENYARAFQAAPFGRYYLNSLVVAVAVTIGQLITCSMAAFAFA
                     RLRFRGRDALFLLFLGTMLIPGQVTMIPTFMVLHWIGWIDSYKALIVPGLASAFGTFL
                     LRQFFMTIPRELEEAAYIDGCSKWRALWHIILPLSRPALATLAIFTFMGVFNDFMWAL
                     IVLSSEEMRTVQLGLAIFRDRYLTEWDLMMAGSVTATLPILVVFFFAQKYFIKGITLS
                     GLKE"
     gene            complement(2025..2921)
                     /locus_tag="PKM23_09855"
     CDS             complement(2025..2921)
                     /locus_tag="PKM23_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY91818.1"
                     /translation="MTRRTKTASNRLQGRDGLHALLFLSPTLVIFTTFVIFPVFFSFY
                     LSFHQWNMFSSQTSFVGLANYSRMLRDPEFWTVVKNTLVYTLGTVPLNMAISLLVAFM
                     LNQKVFGKKFLRTAFFAPVIISPVAAAVIWRWMYDPNFGLVNYMLSFAGIRGPNWLNE
                     SHSAMFALILMAVWKTMGTNMVLFSAGLQGIPDTYYEAAEIDGAGAWTKFWRITVPLL
                     SPTTFFIMVMSLISSFQAFDVVYVLTSGGPLGSTKVLVFYLYEHAFKFFEMGYASAIA
                     YFLFITLIILTLLQIKFMKSRV"
     gene            complement(2940..4256)
                     /locus_tag="PKM23_09860"
     CDS             complement(2940..4256)
                     /locus_tag="PKM23_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY91819.1"
                     /translation="MKKLVLCALLLGLLLQTSCGKKEEAAGDGRTVVTFWHSFVSSTV
                     PALNELIAKFEKENPAIQIRAQYIPTGDALIQKLITSVQSKTAPDISWVHSDYLQDLV
                     EADAIYKMDDFIAGPDGLSEAEMADIYPALIQYASWRGTLYSMPMEATNLALLYNRDL
                     FRKAGLDPDRPPQDWRELHEYAKKLTVDADGDGIFEQVGFSLPVFPATGPLGGWMVWQ
                     WLPFLWQAGGELINEEQTSVLYNSEAGVAALRLWQEIYRDLKLSSYTTDYDVAFASQR
                     QAMALDGPWNLPRYATLLKHFDWGFAPLPQGPAKKATIIGGEYLTIFKQSKHPAEAWK
                     FIKWIIQPENQAFWAIKSSYLPINHSVLQVPEFQSYLAAHPAFKVFVDELEYSLASRP
                     IDFHGLEITRHVAEALEKATVGDQDPAAALNESAQKSNALLQSAAK"
     gene            complement(4283..5143)
                     /locus_tag="PKM23_09865"
     CDS             complement(4283..5143)
                     /locus_tag="PKM23_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91820.1"
                     /translation="MRKINRLTTLSLAIIAAAFIHCGDDNDDLLAPYTGSRPLQWLHV
                     TKSTTPDIQWIGGRVAAAGVNRGSRAAFDSTLIWLRTSPDNQIGSYITFGSNPDLAAI
                     SRAGGNPAATLTDSTQYTFWLADKSALTDGRPDTASNAFTFIDTSFVMNILVRGKLGG
                     ERNSQGKELVDIVITRNETMLEDSYTITWTPAEVAFRQIAIRTSSLGGFTDLLWHIVT
                     PDSLPDNIHPPVRIGVAPPGTEEAVAWPESGFIKNKVQILWMTNSKWKVNNFAPTAAG
                     YAWLRLYPFE"
     gene            complement(5265..6293)
                     /locus_tag="PKM23_09870"
     CDS             complement(5265..6293)
                     /locus_tag="PKM23_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNY91821.1"
                     /translation="MSTTITDIATKTHCSVSTVSRVLNKQAKKYRISKEKERVILQAA
                     KELNYRPNHLARGLRLKKTQTIGLVVPDISNPFFAHVTRVIQTFAYSSGYSLIVCNTD
                     ENLQTEIDQIELLRSKGVDGFIIMPVGVRYSHLQTLLEDEIPLVLLDRCFDDLQTNSV
                     IVNNYKGAFEAVEHLLGFGHSRIAIIQGLADTHTNRARLQGYRDALQKYSIPLDENLI
                     VGNDFRKQNGYIETKILLKLEHPPTALFTTSDLITLGALQAINEEGCRIPDEISLVSF
                     DDTDFAPFLNAPLTAVSQPKELMGEIAVKLLIEDLKSQGHKEKKRIVLEPQLIIRKSV
                     AHYPPVHN"
BASE COUNT         1379 a         1685 c         2081 g         1349 t
ORIGIN      
        1 tgtcggggaa gggcacgccg ccccaggcgc cgtcccagaa gccgatcatg ttgaccgctg
       61 ccgcgttggt ccatgcggcc tcgtcaccca cgccgtcgaa gacgatggcg tcgggagcga
      121 cccaggggac atcaatatta tcggcgatgc cgtagggctc cggaccaccg acaaaggtca
      181 gggtgccaaa gctctgggcg cggcgctgga tatcgccggc gatatagtcg ctgggatagc
      241 cgaccgcagt gtcagcgggc tggggatgcc aggcatagta ggcataggtg ccttcgcccc
      301 attcccatgg gacagctgtg gtgtcaggag tcgccgccgc cccgccgata ttgaagccga
      361 ccatggaatt ggtgcggatc tgcggcagcc agatgcccat ctcaacgccc caggtcagat
      421 cgtcgttgtt gacaaaaacc tggccggcaa cccagccgga atcgatgggc gccagttccc
      481 accaggtctg gttgggatca tcccaccagc cgccccagtt gagagtaatg cccttgtcgt
      541 tgatcttgta gacggtcggg cccttgtccg gggcattcca gggggcgccg ctccagtcat
      601 cagcatagtt gccgcccggg ggatcgccgg cgaggatgcg atcgaccccg accatgatct
      661 gctcgccgat ccagttggcg gtgtcaggac cgaaaaagaa ttcataatcc tcgactttgg
      721 cgtagatgta gagccagtcc tcggaccaga gcagtttggc cttggcttcg atgtcgggga
      781 agggcacgcc gccccaggcg ccgtcccaga agccgatcat gttgacttcg acagcgctgt
      841 tccaggcgga ctcattgcca ttgccatcga gcgtcagggt tccgggagcc acatagggaa
      901 catcgatgtt gtcgcccacc ccgtagggcg cctgcgcacg cacgacagcc aggccggtga
      961 ccagaaacag acagagcagg agtgtcaatt ttttcattgc taacctccca acagtttgag
     1021 cttcagtgta gccggttaat tacggatgag aaagcatgac agtgccctct tcctgcatca
     1081 cctccttgtc atcacttggt tcgctgctgc tccccaattt tcagtggtct acagagtccg
     1141 gcgcttgcgg catcacccgg tgtgccttgt ccctcactcc ttcagtcccg acagggtgat
     1201 gcccttgata aaatatttct gggcgaaaaa gaagacgacc agaatgggca aagtagcagt
     1261 caccgaaccc gccatcatca gatcccattc tgtaagataa cggtcacgaa atatggccag
     1321 ccccaactgc acggtgcgca tctcttcgga gctgagcacg atcagggccc acatgaaatc
     1381 gttgaatacc cccatgaagg taaaaattgc cagggtcgcc agggccggcc gggagagggg
     1441 aaggatgatg tgccagagag cccgccattt gctgcagccg tcaatgtagg cggcctcctc
     1501 gagctcgcgg gggatggtca tgaaaaactg ccgcaggaga aaggtgccaa aggctgaggc
     1561 cagaccgggg acgatgagcg ccttgtagct gtcgatccag ccgatccagt gcagcaccat
     1621 gaaggtgggg atcatggtca cctggccggg gatcagcatg gtgccgagaa aaagcaaaaa
     1681 gagggcatcc cggccgcgga acctcagccg ggcaaatgcg aaggcggcca tcgagcaggt
     1741 gatgagctgg ccgatggtga cagcgaccgc cacaaccagg ctgttgagat agtagcgccc
     1801 gaagggggcg gcctgaaagg cccgggcgta attctcgaaa tggaaggttt ttggcagcca
     1861 cctgggcgga tagacaaaga tttcgttgag gtccttaagt gaggtcagca ccatccagat
     1921 gaaaggcgcc agggtcgcca gggccgcccc gtagatgaag aggtagaata gggtacgctt
     1981 gctgatgtga aggatggttc tggctgtcgg tttcatgggc ggcatcagac tcgtgatttc
     2041 atgaatttga tctgcagcag ggtcaggatg atcagggtga tgaagaggaa ataggcgatc
     2101 gccgaggcgt agcccatttc aaagaacttg aaagcgtgct cgtagagata aaagaccagc
     2161 accttggtcg aacccagcgg ccctcccgag gtgagcacat agacgacatc gaaggcctgg
     2221 aaagagctga tgagggacat caccatgatg aaaaaggtgg tcggactcag caggggcaca
     2281 gtgatgcgcc agaatttggt ccacgccccc gccccgtcga tctcggcggc ctcgtagtag
     2341 gtatcgggga tgccttgcag cccggccgaa aaaagcacca tattggtccc catcgtcttc
     2401 cataccgcca tcaggatgag ggcgaacatc gccgaatggg attcgttcag ccagttgggt
     2461 cccctgatcc cggcgaagga gagcatatag ttgaccaggc cgaaattggg atcatacatc
     2521 cagcgccaga ttacggcggc ggccaccggt gagatgatga ccggggcgaa aaaggcggtg
     2581 cggagaaatt tcttgccgaa gaccttctgg ttgagcataa aggcaaccag cagggagatg
     2641 gccatattga gcggcactgt gcccagggtg tagaccaggg tgttcttcac caccgtccag
     2701 aattccggat cgcgcagcat gcgagaataa ttggcgaggc cgacaaagga ggtctgcgaa
     2761 ctgaacatat tccactgatg aaagctgaga taaaaggaga aaaaaaccgg aaagatgaca
     2821 aaggttgtga aaatgaccag cgtcggcgac aggaagagca gggcatgaag tccatccctg
     2881 ccctggaggc ggttgctggc ggtttttgtt ctccgggtca cggtcatcag tggggctctc
     2941 tatttggccg cgctctggag cagggcattc gatttttgcg ccgactcgtt caatgcggcg
     3001 gcggggtcct gatcgccgac cgtagccttt tcgagggcct cggcgacgtg acgggtgatc
     3061 tccagtccgt gaaaatctat cggccgcgaa gccaggctgt attcgagctc atcgacgaag
     3121 accttgaaag cggggtgggc cgccagatag ctctggaact cgggcacctg cagcaccgaa
     3181 tggttgatcg gcagatagct cgatttgatc gcccagaagg cctgattctc gggctgaatg
     3241 atccatttga tgaatttcca ggcttcggcg ggatgcttgc tctgtttgaa gatggtcaga
     3301 tattcgccgc cgatgatggt cgcctttttg gccggcccct gcggcagggg agcaaaaccc
     3361 cagtcgaaat gcttcaacag ggtggcataa cgcggcaggt tccaggggcc atccagggcc
     3421 atggcctgac gctgggaagc gaatgcgacg tcgtaatcgg tggtgtagga actcagcttc
     3481 agatcacggt agatctcctg ccagagccgg agggcggcga cgcctgcctc actgttgtag
     3541 aggacgctgg tctgctcctc attgatgagc tctccccccg cctgccagag gaagggcagc
     3601 cactgccaga ccatccagcc gccgaggggg ccggtggcgg gaaaaaccgg cagtgaaaag
     3661 ccaacctgtt caaagatgcc gtcgccgtcg gcgtccacgg tgagcttttt cgcatactca
     3721 tgcagctcgc gccagtcctg aggaggacgg tcgggatcaa gccccgcctt gcggaagagg
     3781 tcacggttgt agagcagggc gagattggtc gcctccatcg gcatgctgta gagggtgccg
     3841 cgccaggagg cgtactggat gagggcggga tagatgtcgg ccatctccgc ctcagagaga
     3901 ccgtcgggcc cagcgatgaa atcatccatt ttgtagatgg cgtcagcctc gaccagatcc
     3961 tgcagataat cggaatggac ccaggagatg tcgggcgcgg ttttgctctg caccgaggtg
     4021 atcagcttct ggatcagggc atcgccggtg gggatgtact gcgccctgat ctggatggcc
     4081 ggattttctt tttcgaattt ggcgatcagc tcattgagcg cgggtacagt ggaggagacg
     4141 aagctgtgcc agaaggtgac gaccgtccgg ccatcgccgg ccgcctcttc tttttttccg
     4201 caggaggtct gcaggagcag ccccaacagg agggcgcaaa ggacaagttt tttcaaggtc
     4261 gcatcctttc gtgaaggggg catcattcaa agggatagag gcgcagccag gcgtagccgg
     4321 cggccgtggg ggcaaagttg ttgactttcc atttgctgtt ggtcatccaa aggatctgga
     4381 ccttgttttt gatgaatccg ctctccggcc aggcgacagc ctcttcagtg cccgggggtg
     4441 caacgccgat cctgaccggg ggatggatgt tgtcgggcag ggaatcgggg gtgacgatgt
     4501 gccagaggag atcggtgaaa ccacccagcg agctggtccg gatggcgatc tgccggaaag
     4561 cgacctctgc cggggtccag gtgatggtat agctatcctc gagcatggtt tcattgcggg
     4621 tgatgacgat atcgacgagt tccttgccct ggctgttcct ttcgccgccg agtttgcccc
     4681 ttaccaggat gttcatcacg aaggaggtgt cgatgaaggt gaaggcgttg ctggcggtat
     4741 cggggcggcc gtctgtgagg gcgcttttgt cggcaagcca gaaggtatac tgtgtggagt
     4801 cggtcagagt ggcggcgggg tttccgccgg ccctgctgat ggcggcgaga tcgggatttg
     4861 agccgaaggt gatatatgag ccgatctggt tatcggggct ggtgcgcagc cagatcaggg
     4921 tagagtcgaa ggcggcgcgg ctgcccctgt tgacaccggc ggcggcgacg cggccgccga
     4981 tccactggat atcgggagta gtgcttttcg tgacgtgcag ccactgcagg ggtcggctgc
     5041 ccgtgtaggg tgcgaggagg tcatcgttat cgtcgccgca gtggatgaaa gcggcggcga
     5101 tgatggccag ggacagggtg gtgaggcggt tgatttttcg catgggagaa atcctcaagc
     5161 ctgggttatg aaaacgtttg cgcaaaatag tcccattcca ttcacttcat catttcatgg
     5221 tattaagcgg tcacaggtag aacatgacag cgcttggaac tggttcagtt gtggactggt
     5281 ggatagtgcg cgacggattt gcggatgatg agttgaggtt ccagtacaat gcgctttttc
     5341 tctttatggc cctggctctt gaggtcttcg atgagaagtt tgacagcgat ctcccccatc
     5401 agttccttgg gttggctgac ggcggtaaga ggggcgttaa gaaagggggc gaaatcggta
     5461 tcgtcgaagg agaccaggga gatctcatcg ggaatcctgc agccctcttc attgatcgcc
     5521 tggagtgcgc ccagggtgat aaggtcgcta gtggtgaaga gggcggtcgg cgggtgttcg
     5581 agcttgagca ggatcttggt ttcgatatag ccgttctgtt ttctaaagtc attgccaacg
     5641 atgaggttct catcgagggg aatgctgtat ttttgcaggg catcccgata gccctgcaga
     5701 cgagcccggt tggtgtgggt gtccgccagc ccctgaatga tggcgatgcg ggaatggcca
     5761 aaaccgagca gatgttcaac cgcctcgaag gcacctttgt aattatttac aattactgaa
     5821 tttgtctgca ggtcatcgaa acagcggtcg agtagcacca gcgggatttc atcctccagc
     5881 agggtctgca ggtgagaata gcgtacaccg acgggcatga taataaagcc gtcgacgccc
     5941 ttgctcctca acagctcgat ctggtcgatc tcggtctgca gattctcatc ggtgttgcag
     6001 acgatcaggc tgtagccgga actgtaggca aaggtctgga tgacccgggt gacatgggcg
     6061 aaaaaggggt tcgagatgtc ggggacgacc agaccgatgg tctgggtctt tttaaggcgc
     6121 aggcctcgcg cgagatggtt ggggcggtag ttcaattcct tggcggcctg caggatgacc
     6181 cgctccttct ccttgctgat gcggtatttt ttggcctgct tgttgagcac tcgcgagacc
     6241 gtggataccg aacagtgggt tttggtggcg atgtcggtga tggtggtgga catgagagcg
     6301 ccgtcccgtg ctgggggtgg aggaactcgc cgcaaaggat agaacaaacg tttgtgcaat
     6361 taagtatagc aaactcgccg ggcattgtca agcacttttt acaaaatctc catttttctg
     6421 ctgcctcgag ctgcacattg cggctaaatt tcacaggcat gcccccctcc cttccattgc
     6481 agagaattct ctca
//
LOCUS       DAOPWU010000186         6483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_11844_length_6483_cov_4.383012, whole genome shotgun sequence.
ACCESSION   DAOPWU010000186 DAOPWU010000000
VERSION     DAOPWU010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11844_length_6483_cov_4.383012"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /gene="pheT"
                     /locus_tag="PKM23_09875"
     CDS             <1..1434
                     /gene="pheT"
                     /locus_tag="PKM23_09875"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNY91822.1"
                     /translation="GLNSEVEAETRTLFLESAYFEPTGIRKSSKRLGISTESSKRFER
                     GADPNGTLYALDRAASLIMELAGARPAGEAVDVYPAPIAPALIDLSLDHVNRLIGTEL
                     TAAEAERILTSLEIACEHTSTGSISCMVPTFRPDLTRPVDLIEEIARVYGFDRIPFAR
                     TVILDQSQTPNPRTAFQDEVRRLLAGYGLRETLSLSLVSPQAAAPFLGEGCSQVELLN
                     PLSTEWSVFRTSLLISLLQNAAYNRNRQMPNLRFFEIGSAAWKRGEAIEEKKQIAALL
                     VGETGLPTWHSRPRRFDFYDIKGIALALLEALGLKGAVMGSAAGSFWACESAGLYLQG
                     KFLGCFGRLDDELVQSFKIKTPDVFGLQLEFDTLYTLRPQVHRFEAIPRFPLVPFDIA
                     LLLDSDTPVGDVEEAIRKTGGEHLVSVHLFDFYQGEQVPQGKKSVAFSLNFCSKERTL
                     GVEEVERAVATILAQLAGQFGAVLRQR"
     gene            1447..1740
                     /locus_tag="PKM23_09880"
     CDS             1447..1740
                     /locus_tag="PKM23_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91823.1"
                     /translation="MDKNILDILEERIQYALGLISEMRQKNFLLEQENSDLKRRLAEQ
                     NQQLDQARQQFNEQSNRAEQEMLSKYRETEERLRERVQNMLIKLDELKSFENR"
     gene            1803..2123
                     /locus_tag="PKM23_09885"
     CDS             1803..2123
                     /locus_tag="PKM23_09885"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNY91824.1"
                     /translation="MIDEKNILRVNIFGTDYPLKVNANVEYVQRIAEYVDQKMREVQT
                     AKPNRPLHQIAILAALNIADELLQQRELSKKKLSSFEDRVRILTHKFDIDIQELMEEG
                     KKGV"
     gene            2314..3876
                     /gene="rny"
                     /locus_tag="PKM23_09890"
     CDS             2314..3876
                     /gene="rny"
                     /locus_tag="PKM23_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010023075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNY91825.1"
                     /translation="MNTYLVLFIAIADAIVAFVAGWLVSRHIGRGKVIDAEKLAEKIL
                     LDAKKEAENLRKEKTLEAKDEIFKLRQNLENEIKLRRSDLQKMENKLTARELNLDRRH
                     DLLTNKEKELDEIENNLKGREERVQKREGELDELIEEQNRRLEKVSGISNEEAKRILM
                     QNLIEKSKQEAAQSIKEIKDRARQQANREAKEIIIQAIQRTAANHSTETTVSVVNLPS
                     DEMKGRIIGREGRNIRSFESATGVEVIVDDTPEAVVLSGFDPFRREIARISLERLVVD
                     GRIHPTRIEEVVEKTKQEMEEKLVEEGEKALMECGIASMHIELVKLLGKLNYRTSYGQ
                     NVLQHAVEVSYLTGLMAATLGLDGNLAKRAGLLHDIGKAIDRYTDGTHTQIGLEIAKK
                     YGEGPIVQNAIAAHHEDVEIISPISVLVQAADAVSGSRPGARRETLETYIKRLDSLEG
                     LAKSFAGVGKAYAIQAGREIRVMVEHDKIDDAMSEQLATDIAEKIQNDMEYPGQIKVT
                     VIREYRAIDYAK"
     gene            3927..4718
                     /locus_tag="PKM23_09895"
     CDS             3927..4718
                     /locus_tag="PKM23_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNY91826.1"
                     /translation="MSNKSFNILFIADIVGKPGMDVLAGLLPGLKKSHNVDFCVANGE
                     NADGGNGLIEALARQLFGLGVDVITGGNHTWGNAQFRAYLDNSQKVLRPLNYPPAAPG
                     HGSVVVTSTRQIPVAVINLQGRTFMYSIDCPFRTGHEEVARMREKAAIILIDFHAEAT
                     AEKAALAWYLDGSVSAIIGTHTHVQTADERILPRGTAFITDVGMTGPHDSVIGMEAQV
                     AIKRFTTILPEKYRIASANVRLNGALIEVDPQNGRALRISRINVP"
     gene            4735..4808
                     /locus_tag="PKM23_09900"
     tRNA            4735..4808
                     /locus_tag="PKM23_09900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4769..4771,aa:Arg,seq:ccg)
     gene            4956..6323
                     /locus_tag="PKM23_09905"
     CDS             4956..6323
                     /locus_tag="PKM23_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY91827.1"
                     /translation="MDALKTISVTIAAAELNRLYPDLDPAAEADKRMLLSRLEERYRG
                     GGDRLELSWYNRDLKITWGVKPPAPEAERLNREALEKARQKALEAALSLWRKAIEWSP
                     RDPDLHYNMGLVYFESKEYTKGLDKCIETVRLCPIYARAYFVLGSLYSKMRQFAQSER
                     FLKEGLILQPANVMALVNLGAVYSIQKNYGEAIRAFEKSIAVSPKEVRSYLGLGKVYM
                     AQGDMDNAGRCFKAVLKLDPEGKFAEIARKSLRMVTPEMPAGNAGTVVSDENFEAICA
                     EGFKAYIAGDYMSAVVFYQRYLAQRSGEADIWASLASCQIRLGQGDAAMEAIRNAIKY
                     APNKPAFYKQAGIIFDACEHDGEAGQYALKAIDLGKQDSVTLTLAGKSLLVTGKAQEG
                     LRYLADAVKANPNNLHARFYYAQALRSTGDLESARQNLEEILWSKVETPLKERARREM
                     QALAG"
BASE COUNT         1519 a         1838 c         1843 g         1283 t
ORIGIN      
        1 ggcctcaatt cggaagtgga ggcggagacg cgcaccctct tcctggagag cgcctatttt
       61 gaacccaccg gcatccgcaa atcgagcaaa cggctcggca tctcaaccga atcctccaaa
      121 cgtttcgaac gcggggccga tcctaacggg accctctatg cccttgaccg ggcagcttcc
      181 cttatcatgg aactcgccgg agccaggccg gccggtgagg ctgtcgatgt ctatccggcc
      241 ccgatcgcac ccgctctcat cgacctctcc ctggaccatg tcaatcgcct catcgggacg
      301 gaactgacgg ccgctgaggc tgagcgcatc ctcaccagcc tggaaatcgc ctgcgaacat
      361 acctccaccg gcagcatttc ctgcatggtg ccgactttca ggccggatct cacccggccg
      421 gtggacctca tcgaggagat cgccagggtc tacggatttg accgcattcc cttcgcccgc
      481 acggtgattc tcgaccagtc ccaaacgccc aatccccgca ccgcctttca ggatgaggtg
      541 cggagactcc ttgccggcta tggactgcgc gaaaccctct cgctcagtct ggtgtcgccg
      601 caggcggccg ctccctttct gggtgaagga tgcagccagg tcgaactgct caatcccctc
      661 agcacggagt ggtcggtttt tcgcaccagc ctgctcatct ccctgctcca gaacgctgcc
      721 tacaaccgca atcggcagat gcccaacctg cgcttctttg aaatcggcag cgcggcctgg
      781 aaaagggggg aggccattga agaaaagaaa cagatcgccg cgctgctggt cggggagacg
      841 gggctgccca cctggcactc ccgcccgcgc cgcttcgatt tctatgatat caaagggatc
      901 gccctcgctc tgctcgaggc tctcggattg aagggcgcag tgatgggcag cgcagccgga
      961 tccttttggg cttgcgagag cgccgggctc tacctgcagg ggaaatttct gggctgcttc
     1021 ggacgcctgg atgatgaact ggtgcaatcc ttcaagatca agactccgga tgtcttcggc
     1081 ttgcagctgg aattcgatac actctatacc ctgcgcccgc aagtgcaccg gttcgaggcc
     1141 attccccgct tcccgctggt gcccttcgat attgcgctgc ttctcgatag cgacactccg
     1201 gttggcgatg tcgaggaggc catccgcaag acgggaggag agcatcttgt aagtgtccat
     1261 cttttcgact tttatcaggg cgagcaggtg ccgcagggga aaaaaagtgt cgctttttca
     1321 ttgaattttt gttctaaaga gcgtacatta ggagtagaag aggtggagcg cgctgttgcc
     1381 accattctcg cacagttagc cgggcaattt ggagcggtct tgcggcaacg ttaggacgag
     1441 gttcgtgtgg acaagaacat actggatatt ctggaagagc gcattcagta tgcgctcggg
     1501 cttatttcgg aaatgcgaca aaagaacttt ttgttagagc aggagaacag tgatctgaaa
     1561 aggcgtcttg ccgaacagaa tcagcagctc gaccaggcgc gccagcagtt caacgagcag
     1621 agcaatcgcg ccgaacagga gatgctgtcg aaatatcggg agaccgagga gaggctgcgc
     1681 gaacgagtgc agaacatgct gatcaagctt gatgagctga aatcatttga gaatcgctag
     1741 ttatagtcgg agtttccaac acaacttaag ctgtcagatg agccttttgc tggcctactt
     1801 tcatgattga tgaaaaaaac atcctcagag tcaatatttt tggaacggat tatccgctca
     1861 aggttaatgc taatgtggaa tacgtgcagc gcatcgccga atatgtagat cagaagatgc
     1921 gcgaagtgca gacggccaag cccaatcgtc cccttcatca aatcgccatc ctggccgccc
     1981 tgaacatcgc cgatgagcta ctgcagcagc gggaactatc caaaaagaaa ctctcttcat
     2041 ttgaggatcg ggtcagaatc ctgacccaca agtttgacat cgatatccag gaactcatgg
     2101 aggaaggcaa gaaaggcgtt tgatctgtcc ggcccgcttc agggccggat acattcattc
     2161 ggtgtttttt aaaacccagc tgtcaagagt ccagttggaa attccacatt gcggagcacc
     2221 ccctgatcag agggtacttc acgggaattt cccgtctaat ttaccataga atggatccct
     2281 gacaaccctg ggttttttag ttccggaggg taaatgaata cttatttggt gctttttatt
     2341 gctatcgccg acgcgattgt agctttcgtc gccggctggc ttgtttcccg ccatatcggg
     2401 cgcggcaagg tgatcgatgc agaaaaactg gcggagaaaa ttctcctcga tgccaagaaa
     2461 gaggctgaaa atctcagaaa agaaaaaacc ctcgaagcca aggatgagat cttcaagctt
     2521 cgccagaatc tggaaaacga gatcaagctg cgtcgcagcg acctgcaaaa gatggaaaac
     2581 aagctcacgg cgcgtgagct aaatcttgac cgcaggcatg atctcctcac caataaggaa
     2641 aaagagctgg atgaaatcga aaataatctg aaggggaggg aggagcgtgt ccagaagcgg
     2701 gagggggagc ttgacgaact gatcgaggag cagaacaggc gcctggaaaa ggtgagcggc
     2761 atctccaacg aagaggccaa gcgcattctc atgcagaacc tgatcgaaaa gtccaagcag
     2821 gaggccgccc agtccatcaa ggagatcaag gaccgcgccc gtcagcaggc caaccgcgag
     2881 gccaaggaga tcattattca ggccatccag cgcactgccg ccaaccactc caccgaaacc
     2941 accgtctcgg tcgtcaatct tcccagcgac gagatgaagg gacgaatcat cggccgcgag
     3001 ggacgtaata tccgctcttt cgaaagcgcc accggggtgg aggtgattgt ggatgacacc
     3061 cccgaggcgg tcgtactgtc cgggtttgat cccttccgcc gtgaaatcgc acgcatctcc
     3121 cttgaacgac tggtcgtcga cggccgcatc catccgacgc gcatcgagga ggtggtggaa
     3181 aaaaccaaac aggagatgga ggagaagctg gtcgaggagg gcgaaaaggc cctcatggag
     3241 tgcggcatcg ccagcatgca catcgaactg gtcaaactgc tcggcaagct caattatcgc
     3301 accagctacg gccagaatgt cctccagcac gccgtcgagg tttcctatct gaccggcctg
     3361 atggcagcca ccctggggct ggatggcaac ctggccaaac gcgccggtct gctccacgac
     3421 attggcaagg cgatcgaccg ttatacggat ggcacccata cccagatagg gctcgagatc
     3481 gccaaaaagt acggagaggg gcccattgtc cagaatgcca tcgccgccca tcacgaagac
     3541 gtcgagatta tctcaccgat ctcggtgctg gtgcaggcgg ccgacgccgt cagcggctcc
     3601 agacccggtg cgcgccgtga aacccttgaa acctacatca agcgtctcga cagcctcgag
     3661 ggactggcca aatccttcgc cggcgtcggc aaggcctatg ctatccaggc tggacgggaa
     3721 atccgcgtca tggtcgaaca cgacaagatc gatgacgcca tgtccgaaca gctggcgacc
     3781 gacatcgctg aaaaaatcca gaacgatatg gaatatccag gccagatcaa ggtgaccgtg
     3841 atccgtgagt accgcgctat cgactacgcc aaataggaac aaaaagcttg tgaatgtgcc
     3901 ggaaacggcg tatattaagg ccgatcgtga gcaacaagtc ctttaacata ctctttatcg
     3961 ccgatatcgt cggcaaaccc ggcatggatg tcctcgccgg gctgctgcct ggcctgaaaa
     4021 agagccacaa tgtagatttt tgcgtcgcta acggcgagaa cgccgacgga ggcaacgggc
     4081 tcatcgaggc cctggcgcgg cagcttttcg gcctgggcgt ggatgtcatc accggcggca
     4141 accacacctg gggcaacgcc cagttccgcg cttatctcga caacagccag aaagtcctgc
     4201 ggcccctcaa ctacccgccg gcggccccgg gccacggctc ggtggtcgtg acctccacgc
     4261 gccaaatccc ggttgcggtc atcaatctgc agggcagaac cttcatgtat tcgatcgact
     4321 gccccttcag aaccggacat gaagaggtgg cgcggatgcg cgaaaaggcg gcgataattc
     4381 tgatcgactt tcacgccgag gcgaccgccg aaaaagccgc cctggcctgg tatctggatg
     4441 gctcggtcag tgcgatcatt ggcacccata cccatgttca gaccgccgac gaacgcattc
     4501 tgcccagggg aacagccttc atcaccgatg tcggcatgac cggtccgcac gattcggtga
     4561 tcggcatgga ggcccaggtg gccatcaagc gcttcacgac catattgccg gaaaagtacc
     4621 gcatcgccag cgctaatgtc cggctcaacg gcgccctgat cgaggtcgat ccccagaacg
     4681 gccgcgccct caggatcagc cggataaatg ttccctgaca tgcagataga tctggcgcca
     4741 gtagctcaat aggatagagc gttagcctcc ggagctaaag gttgtgggtt cgattcccgc
     4801 ctggcgcaca aaaaaaggcg gctcgccgga gccgcctttt ttattgattt tatagaaaat
     4861 tattcgtaaa atgcataaag tggagccgcc tgcgggcggc cacaggcgtg tgtaaaccga
     4921 gcgatcgtgc agccatgaca gataatcaag caccgttgga cgcgctgaag accatctccg
     4981 taaccatcgc cgccgcagag cttaaccggc tctatccgga tctcgatccc gctgcggagg
     5041 ccgacaaacg catgctgctc tcccgcctgg aagagcgcta tcggggggga ggcgaccgcc
     5101 tcgaactttc ctggtacaac cgcgatctca agatcacctg gggggtgaaa ccacccgctc
     5161 ccgaagctga acggctcaac cgcgaagccc tcgaaaaagc ccggcagaaa gccctcgagg
     5221 ctgccctttc cctgtggcgc aaggccattg aatggagtcc ccgcgatccc gacctgcatt
     5281 acaacatggg actggtctat tttgagtcca aggagtacac caaaggactc gataagtgca
     5341 tagaaacagt caggttatgc ccaatttatg cccgcgccta tttcgtgctg ggcagcctct
     5401 acagcaagat gcgccagttc gcccaatccg aacgttttct caaggagggg ctcatcctgc
     5461 agccggccaa tgtgatggcc ctggtcaatc tcggagcggt ttacagcatt cagaagaact
     5521 acggcgaggc gatccgcgcc tttgaaaaat cgattgccgt atctcccaag gaggttcgtt
     5581 cctatcttgg cctcggtaaa gtgtatatgg ctcaaggaga tatggataat gctggcaggt
     5641 gtttcaaggc ggtgttaaag ctggatcctg agggcaaatt cgccgagatc gcacgcaaat
     5701 ccctgcgcat ggtcaccccg gagatgcccg ccgggaatgc cggcacggtg gtgagtgatg
     5761 agaattttga ggccatctgt gccgagggtt tcaaggccta tattgctggc gactatatga
     5821 gcgctgtcgt cttttatcag cgctatcttg cgcagcggtc aggtgaggcg gacatctggg
     5881 cttcgctggc ctcgtgccag atcaggctgg gccagggcga cgccgccatg gaggccatcc
     5941 gcaacgccat caagtacgcc cccaacaagc ccgcatttta caagcaggcg ggcatcattt
     6001 tcgacgcctg tgagcatgac ggagaggcgg ggcagtatgc cctcaaggcg atcgatctgg
     6061 gcaagcagga cagcgtgacc ctgacgctgg caggcaagag cttgctggtc acgggcaagg
     6121 cccaggaggg gctgcgttat ctggcggatg cggtcaaggc caatcccaac aatctgcacg
     6181 cccgttttta ttacgcacag gctttgcgca gcaccggtga tctggaatca gcgcggcaga
     6241 atctggaaga gatactctgg agcaaggtgg agacgccgtt aaaggagcgt gcgcgcaggg
     6301 agatgcaggc cctggccggg tgaggtcaag gccgccggga tggagcttcc agggctctgg
     6361 tcatgcgttt gctggttatg cataatatta gttatgttaa tattcgcttg gtcgatagag
     6421 catttcaggc tgctgctccc ctctgtctcg caaaagtccc tctgcgcata taacagaaaa
     6481 agt
//
LOCUS       DAOPWU010000187         6448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_11938_length_6448_cov_5.371187, whole genome shotgun sequence.
ACCESSION   DAOPWU010000187 DAOPWU010000000
VERSION     DAOPWU010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11938_length_6448_cov_5.371187"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PKM23_09910"
     CDS             complement(<1..579)
                     /locus_tag="PKM23_09910"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HNY91828.1"
                     /translation="MEYQITEQSNWHRVVQFAVPAADVKPQLEQKYAEAMKSVRLEGF
                     RKGKVPPGLVKKMFGKSIESEIFNGYVRQALDRLFKENTFDYLNTPYVQDLAWDEEKG
                     LSFALHFDIRPDFQVDGYAGMQVERTLFEASDEDLEHTLADLQSRQAMIYTVEGEAQP
                     GHFIMADLQEVDRTGVPMVGHKYENEVIWLHDD"
     gene            complement(708..790)
                     /locus_tag="PKM23_09915"
     tRNA            complement(708..790)
                     /locus_tag="PKM23_09915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(754..756),aa:Leu,seq:tag)
     gene            complement(914..2935)
                     /locus_tag="PKM23_09920"
     CDS             complement(914..2935)
                     /locus_tag="PKM23_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNY91829.1"
                     /translation="MGFSWLLVSGLSAQADPGPFPRIMFQRPAGISGGAIQHFFSRFD
                     LVAHGGAGPSAYALNESIHALNPGTIILGTSRQGVWPGSFPPECFIYRPNVAELTRAA
                     RPGDREIFVTSTAGFPPASEKYRYAMIGGDEWFTYTDLTPTSFTGVSTSGDFYLTRIH
                     PVGDSIKTPIRFVGFGMLQNVTPFAHLVEGKPVWQYFVDKRFDPAKQDFSYFDGVFYD
                     AYRTFFYPDDIAGGIDLDYNRVDDFEEHGLKWVNAQWADGVKKMLTYERQKLQEVNPG
                     QYPLIALNTGSALEDYSLEVCDGMMWEGFMRFAESWEEMVRVNRVWENAHNPVYTMIE
                     DYDPEKRRAYAKNKFAYMRYGLTTALMAGAFYGRTFGDYYYISLYYDEFDSDLGLPTS
                     PPQKLASGAWARFFDKGVAISNPTGSIITVTDGELRAASGYAGPYYRFLGGQDPVTNN
                     GERFQSIRLEGTLESPPKYIKGDGILLFTQPDTIVSDIMIGNCFNNDTSPGNEKLELS
                     GAWTEIKDPSTQVFDENRNPCYSQWSDDSEDGIGYAAAYNPAQTATALFTPTINVTGY
                     YEISEWHSWAGDVANSGREASNAAVTVEVNGASRFRGHIDQRVKAGRWNRITVVHLPA
                     GRGSSVRLTNAGADGVVLADAMRFRFLGQSSDVDSEPPSAPRNVRVIRP"
     gene            complement(3334..4635)
                     /locus_tag="PKM23_09925"
     CDS             complement(3334..4635)
                     /locus_tag="PKM23_09925"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HNY91830.1"
                     /translation="MIWSVVTPPFQAPDEDIHFCRAYQLADGDLLPLTAGGTQGGCLP
                     LSVIETITRVNPGLRYHSRDQRQDPGRILHYLQRPLEPGKTRFFAFPSGAFYPPAAYL
                     PHVAAIWTGRLIGCSPLVMMYLGRWFTLLLWLLLVRAAIKITPTGRWIFMAVALMPMS
                     LFIGASFNQDAVTNGLAFFTLAWLLDLSRKPGLLTRGQLALFALLVAAFALAKPGFAL
                     FALLFFLLPRAKARSQRAYLATGGLLLAAGYAAGLVWYNLAGSDLNWNAPFAGYEAQV
                     AALLDNPLLFIRALLRSLWSYKFFYLRSHLGQLGSLDVVLPLHVLLPVLATLAVTACF
                     NKGLLLAARQKAVLALVFLGLLVQAFLALYLTASPVAGPYCHGFQGRYLISVAPMFWL
                     LWQNRLQGLGRRWRELAGPALFVLYLVVLSLATRVLFLRYY"
     gene            complement(4837..>6448)
                     /locus_tag="PKM23_09930"
     CDS             complement(4837..>6448)
                     /locus_tag="PKM23_09930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNY91831.1"
                     /translation="ASGRDDLSTLYTLCRVTVVLFGVGSLWVIYRLGDRLHDYRTGLI
                     AAFLLCLSPLHVINSHFATTDVPLTFFIMLTLWATLRLSDTRALADYILAGIAFGLTV
                     SIKIPGAVIFLAILVAHLHGVAREGHLRYRSILAHEWRNPRKMLLALLGAAAAAAAVY
                     LVFARFSHWAPRLMERIPVELWLKYYDEIAARAAAMAPKMGLVIFTGILVLAYTAKLW
                     IPQLRKLLLLVVVAVLTFFATTPYAILDFKAFAHDFLFQMVISQSSWSGMFADKAPGW
                     ITNATYLYGDFGPLLLLAAVAGLVRQLRARQIRYWILITFALVYYLYIGSWKLMFDRY
                     MVPMLPVIAIWAAWGIVSLIDFIRQRGARSGTGQQHPAGSDSAAEGKGKSSRATNAAL
                     LMTILLLLLIPGAQMLRESWDFNAYLLKTNTKKIAYDWAVTHLPKQSLVLREQYTPEV
                     ELAGYEVHLVNFTFNDSVTADYVRRHHIDYIIVTDKLWKRPVKDNGVLGERKAYAEIP
                     GYADLIYNLKPTPQNPGPEVKIYQVRKE"
BASE COUNT         1344 a         1830 c         2136 g         1138 t
ORIGIN      
        1 gtcgtcgtgc agccagatga cctcattctc atacttgtgg cccaccatcg ggacgccggt
       61 gcggtccacc tcctgcaaat cggccatgat gaaatggccg ggctgggcct ccccttcgac
      121 ggtgtagatc atggcctggc gggactggag atcagcgagg gtgtgttcaa gatcctcatc
      181 gctggcctca aaaagggtgc gttcgacctg catcccggcg tagccgtcga cctggaaatc
      241 gggacggatg tcgaaatgga gggcgaagct cagccccttc tcctcgtccc aggcgagatc
      301 ctgtacatag ggggtattga gataatcgaa agtgttctcc ttgaagaggc ggtcgagggc
      361 ctggcggaca tagccgttga aaatttccga ctcgatgctc ttgccgaaca tctttttgac
      421 cagccccggg ggaaccttgc ccttgcggaa gccttcgagg cggaccgact tcatggcctc
      481 ggcatatttt tgttcaagct gcggtttgac atcggcagcc gggacggcaa actggacgac
      541 ccggtgccaa ttggattgtt cggtgatctg gtactccacc cttgttctcc cttggtttgc
      601 aggtttcgcc gctccgggcg gcgcttcgga atataaaaaa cgccgtctac aggctggatg
      661 ccggggtccc ggaatccagg tgcatcggct gtcaatgcgt agcggcgtgc gagagggggg
      721 actcgaaccc ccaagtcttt gggacactag atcctaagtc tagcgcgtct gccagtttcg
      781 ccactctcgc ggatcgagac ggataaatat agagatttgg cccctcaaag tcaattgctt
      841 tttcccgcac aaaaaggccc ggcgggaaga gaccgccggg cgcaaaaagg aaaacaaagg
      901 ggccgcagag cgctcagggc ctgatcacgc gcacattgcg cggagcggag gggggctcgc
      961 tgtcgacatc gctgctctgg ccaagaaagc ggaagcgcat ggcatcggcc aggaccaccc
     1021 cgtccgcacc ggcattggtg aggcggaccg agctgcccct gccggcgggc agatgaacca
     1081 cggtgatccg gttccagcgt ccggccttta cgcgctgatc gatatggccc ctgaaccggg
     1141 aggccccgtt gacctcgacc gtgacggcgg cgttgctcgc ctcgcggccg ctgttggcca
     1201 cgtcgccggc ccagctgtgc cattcgctga tctcgtaata gccggtcaca ttgatagtcg
     1261 gggtgaaaag ggccgtggcc gtctgggcgg gattgtaagc cgccgcatag ccaatgccgt
     1321 cctcgctgtc gtcgctccac tgcgaatagc agggattgcg gttttcgtcg aagacctggg
     1381 tggagggatc cttgatctcg gtccaggccc cgctcaactc cagtttttcg ttgccgggtg
     1441 aggtgtcgtt gttgaagcag ttgccgatca tgatgtccga gacgatggtg tcgggttggg
     1501 tgaagaggag gatgccgtcc cccttgatgt atttgggggg cgactcgagg gtgccctcga
     1561 gccggatgct ctggaagcgt tcgccattgt tggtcactgg atcctggccg ccgagaaagc
     1621 ggtaataggg tccggcatag ccactggcag cgcgcagttc cccatcggtg acggtgatga
     1681 tgctgccggt gggattgctg atcgcgaccc ccttgtcgaa aaatcgcgcc cacgccccgc
     1741 tggcgagctt ttgcgggggc gaggtcggca ggccgaggtc gctgtcgaat tcatcataat
     1801 agagggagat gtaataatag tcaccgaagg tgcgcccgta aaaggcccct gccatgaggg
     1861 cggtggtcag gccgtagcgc atataggcga atttattctt ggcataggcg cggcgtttct
     1921 ccggatcgta atcctcgatc atcgtgtaga ccggattgtg agcgttctcc cagacgcggt
     1981 tgacccgcac catctcctcc cagctctcgg cgaagcgcat gaaaccctcc cacatcatgc
     2041 cgtcgcagac ctcgaggctg taatcctcga gggcgctgcc ggtgttgaga gcgatgaggg
     2101 ggtactggcc gggattgacc tcttgcagct tctgacgctc gtaggtgagc atctttttaa
     2161 cgccgtcggc ccactgggcg ttgacccatt tcaggccgtg ctcctcgaaa tcatcgacgc
     2221 ggttatagtc gagatcgatg ccgccggcga tgtcatccgg gtagaaaaaa gtgcgatagg
     2281 cgtcataaaa gaccccgtcg aagtagctga aatcctgctt ggccggatcg aagcgcttgt
     2341 cgacgaaata ctgccagacc ggcttgccct ccaccagatg tgcgaagggg gtgacattct
     2401 ggagcatgcc gaagccgaca aaacggatgg gagtcttgat gctgtcgccg acaggatgga
     2461 tgcgggtgag ataaaagtcg ccgctggtgc tgacccccgt aaagctggtg ggggtgagat
     2521 cggtgtaggt gaaccactca tccccgccga tcatggcata gcgatatttt tcggaagccg
     2581 gcggaaaacc ggcagtgctg gtcacgaaaa tctccctatc gccgggcctg gccgcgcggg
     2641 tgagttcggc cacattcggc cggtagatga agcactccgg cggaaaactt cccggccaga
     2701 ccccctgacg gctggtgccg agaatgatcg taccggggtt gagggcatga atgctctcgt
     2761 tgagggcgta ggcgctggga ccggcaccgc catgagcgac cagatcgaag cgggagaaaa
     2821 aatgctgaat ggcgccgccg gagatgccgg cgggccgctg aaacatgatg cgcgggaagg
     2881 ggcccggatc ggcctgcgcg ctgaggccgg aaaccagcag ccaggagaaa cccacacagc
     2941 cccaaaatcc ccgttttgcc cttgccagaa acaccatcat acctccggat tcatttcgcg
     3001 attcaacagg actaaagccc gggccggctg gccattctgc agccccaaca cctaagaaat
     3061 ggcggaaatt gggccacagc ggcggttgtc cgtcccctcc gctccccagc cccgccatcc
     3121 ctcgggagcc gacgctgcgg cgcggccggt tttgccggga gcatcctgcc ttttatttac
     3181 aatatcttac actgcctaca gccgtaccgg cgccggccgg ctcgagccgg tcatagcttc
     3241 acagccttcc ctcctgcaca ggcgccagta ggcctgaata tcgctgcaaa tatcccgcca
     3301 cgctttactt ttttcccccc atgccgtcca atgctaataa tagcgcagga acaggacccg
     3361 ggtcgcgaga gaaaggacaa caaggtagag cacaaagagg gcaggaccgg ccagttcccg
     3421 ccagcgccgc ccgagcccct gcagccgatt ctgccagagc agccagaaca tcggggcgac
     3481 ggagatcagg tagcggccct gaaaaccatg gcagtagggg ccggccaccg gagaggcggt
     3541 cagatagagg gcgaggaaag cctgcaccag caggcccaga aaaaccaggg cgagcaccgc
     3601 cttctgcctg gccgccagga gcagtccctt gttgaaacag gcggtgacgg ccagggtcgc
     3661 cagcaccggc agcagcacat ggaggggaag cacgacatcg agcgaaccga gctggccgag
     3721 atgggagcgc agatagaaga atttgtaact ccacaggctg cgcaggaggg cccggatgaa
     3781 gagcagtgga ttgtcgagaa gcgcagcgac ctgggcctca tagccggcga agggggcatt
     3841 ccagttgaga tcgctgccgg ccaggttgta ccaaaccagg ccggcggcat agccggcggc
     3901 gaggagcagc cccccggtgg ccaggtaggc gcgttgtgag cgcgccttgg cccggggaag
     3961 tagaaaaaag agcagggcga agagggcgaa ccccggcttg gcgagggcaa aagcggcgac
     4021 caggagggcg aacagcgcca gctggccgcg ggtgagcagg cccggcttgc gggagaggtc
     4081 gagcagccag gcaagggtga aaaaggcgag gccattggtg accgcatcct ggttgaatga
     4141 ggccccgatg aaaaggctca tcggcatcag ggccaccgcc ataaagatcc accgccctgt
     4201 cggtgtgatt ttaatggcgg cacggaccag cagcagccag agcagcagtg tgaaccagcg
     4261 accgagatac atcatgacca ggggcgagca gcctatgagc cggccggtcc agatcgccgc
     4321 cacatgcggg agataggcgg caggcggata aaaggccccc gagggaaagg cgaaaaacct
     4381 ggtcttgccg ggttcgaggg gccgctgcag atagtgcagg atccggccgg ggtcctggcg
     4441 ttggtcccgt gagtggtagc gcagcccggg attgacgcgg gtgatggtct cgatgacgct
     4501 gagggggagg cagcccccct gggtcccccc ggccgtcaac ggcagcagat ccccatccgc
     4561 gagctgatag gcgcggcaga aatggatatc ttcgtcgggc gcctgaaagg gcggcgtgac
     4621 caccgaccag atcatcccga aaactgttcc gaggaggaga aaaatccgca cggtggttag
     4681 agaagtaata tatgggcggc aaggaatcat caagtcggcc ttctgcctgc atggcttcat
     4741 gtggaggaat atgggagttc ctgctgcatc ggcgcaggga gaggcgggcc cgctgccggc
     4801 ggcaggggcc cgcacgaagg ttgggatgga gtgaccttat tctttccgca cctggtagat
     4861 tttgacctcg ggtccggggt tctgcggcgt gggtttgaga ttgtagatca gatcggcata
     4921 gccggggatt tcagcataag ccttgcgctc accgaggacg ccgttgtcct tgaccggacg
     4981 tttccagagc ttgtcggtga cgatgatgta atcgatgtgg tggcggcgga catagtctgc
     5041 ggtcacagaa tcgttgaagg tgaaattgac cagatggacc tcgtagccgg ccagctcgac
     5101 ctcgggtgtg tactgctcgc gcaggaccag cgactgcttg ggcaggtgtg tgaccgccca
     5161 gtcataggcg atctttttgg tattggtctt gagcagatag gcgttgaaat cccagctttc
     5221 gcgcagcatc tgcgcccccg gtatgagcag caggagaagg atcgtcatca gaagggcagc
     5281 gttggtggcc cgtgaggatt ttcccttgcc ttcggctgcg gaatctgaac cggcggggtg
     5341 ctgctgcccg gtcccggagc gggccccgcg ctgccggata aagtcgatca gactgacgat
     5401 cccccatgcc gcccagatcg cgatcaccgg cagcatcggc accatatagc ggtcgaacat
     5461 cagcttccag gagccaatat agagatagta aaccagggcg aaggtgatga gaatccagta
     5521 gcggatctgg cgcgcgcgga gctgccgcac cagtccggcc actgctgcga gtagaagcag
     5581 gggcccgaaa tccccgtaga ggtaggtggc attggtgatc cagcccgggg ccttgtccgc
     5641 aaacatgccg ctccagctcg actggctgat caccatctgg aagagaaaat cgtgtgcaaa
     5701 ggctttgaaa tcgaggatcg cgtagggggt ggtggcgaaa aacgtcagga ccgccaccac
     5761 aaccagcagc aggagcttgc gcagctgcgg gatccagagt ttggcggtat aggccagcac
     5821 gaggatgcca gtaaagatca ccaggcccat cttgggggcc atggcggcgg ccctggccgc
     5881 gatctcatcg tagtatttca gccacagctc caccgggatg cgttccatga gccggggggc
     5941 ccagtggctg aagcgggcga agacgagata gacggccgca gcggctgcag cagcgccgag
     6001 gagggcgagg agcatcttgc gcgggttgcg ccattcatgc gccagaatac tacggtagcg
     6061 gagatggccc tcccgggcca caccatgcag atgggcgacg aggatcgcca gaaagatgac
     6121 cgccccgggg atcttgatcg agaccgtcag gccgaaggcg atgccggcca gaatatagtc
     6181 ggccagcgcg cgggtatcac tgaggcgcag ggtggcccac agggtcagca tgataaaaaa
     6241 ggtcagcggg acgtccgtgg tggcgaaatg gctgttgatg acgtgcaggg gactgaggca
     6301 gagcagaaag gccgcaatca gtccggtgcg gtaatcgtgc agccgatcgc ccagccggta
     6361 gatcacccag agcgaaccga ccccgaaaag gaccaccgtg acccggcaca gggtgtagag
     6421 ggtggagagg tcatcccggc ccgaggcg
//
LOCUS       DAOPWU010000188         6425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12004_length_6425_cov_5.256358, whole genome shotgun sequence.
ACCESSION   DAOPWU010000188 DAOPWU010000000
VERSION     DAOPWU010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12004_length_6425_cov_5.256358"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PKM23_09935"
     CDS             complement(<1..1120)
                     /locus_tag="PKM23_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNY91832.1"
                     /translation="MMARKYILTLILWAAAAAAQPLSISSSVDRTSLALNQQLVLTVE
                     LTGEGANSVGRIDLPDVSEFLAMIGSGGTSQNIQIINGKMSVQKSFTYFYQTIKEGSF
                     SIPPVSVTYKGQSISSKPIPLSVSQGAATGGSGSSGSGQGVTGQTQAQPLPASEASDE
                     DLFIRALVNKRQVYQNEPVIVTFRIYTRVNVSGYALSKLPETAGFWSEEIPLPQQPQT
                     RNEVINGRKYVVADLKKMAMFPTSPGRVEIGSLGVDCDVRVQARRRSNDIFDSFFDDP
                     FFGRTVRRTVQSRPVSIEVLPFPEAGKPANFSGLAGKFSIQASIDKKEVTTNEAVTFK
                     VVVSGEGNIKTIPRPNVLLPRDFEQYEPKVTENIQRSGD"
     gene            complement(1158..1886)
                     /locus_tag="PKM23_09940"
     CDS             complement(1158..1886)
                     /locus_tag="PKM23_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY91833.1"
                     /translation="MKPIGLFLLAGALLAAGGAQAQPGRGKIVQGNKLYTEERYDDAL
                     IKYRDAQTQAPERAEAPFNIGAAEYKKNKYEEALKETQAALRSDDRLIQAKSYYNMGN
                     ALFRLNKLPEAIQAYTQSLKLDPTDEDAKYNLEFARKKLKDEAQKQQQNPQQQQQQQQ
                     QQQQQQQQDQEQQDKQEQQQEQQQQEEQQQQEQQEQKAQPRPDEISKEDAARILDALK
                     QDERNQKDSRKMKAPGRITVVKDW"
     gene            complement(1892..2929)
                     /locus_tag="PKM23_09945"
     CDS             complement(1892..2929)
                     /locus_tag="PKM23_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNY91834.1"
                     /translation="MLRFANSYLLHLLWLIPLALIFYYFAFRAKSRALQRFGSSELMK
                     KLAANASPTRQILKAALILLALLFALLALARPQIGTRIEEVKREGIDIMIAIDVSTSM
                     MAEDVQPSRLEKAKHEVESLLNRLQGDRVGLIAFSGTAFVQCPLTLDYGAAKLFLDIL
                     DPGLIPTPGTNLGQAISRALEAFDQQERKHKVLVIITDGEDHEGDVMKQAEEAERQGV
                     IIYTVGIGSPKGDPIPVMTDYGVSAGFKKDREGQVVITKLDEVTLEKIALQTNGKYFR
                     ATSGEEELRKIYDDVDKLEKKELGSMRFSQFEDRFQYLLIIAIALLAAEIFVPERRQR
                     RDFWLKRLFKW"
     gene            complement(2945..3937)
                     /locus_tag="PKM23_09950"
     CDS             complement(2945..3937)
                     /locus_tag="PKM23_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNY91835.1"
                     /translation="MFRFANPEFLFLLLILPLMLFWYLRRSLRSRSTVRYSDIALIKE
                     VGTSSRRRYRHLLFLLRLAALALMIIAFARPQAGSREEEMITEGIDIVLSMDISSSML
                     AEDFQPKNRLEAAKLVAADFIKGRSNDRIGMVVFAGQSFTQCPLTLDYGILLKFLEEI
                     HIGQIEDGTAIGMGLGTSINRLRDSKARSKVVILLTDGQNNRGELDPITAARIAKSFN
                     IRVYTIGAGSKGEALYPVDDPIFGRRYVRMPVNIDEDLLRRVAEITSGQYFRATDKTS
                     LERIYKEISAMEKTKIEVKQYTRYRELFSRYLIAALILLALEVILGNTIFRKIP"
     gene            complement(3939..4985)
                     /locus_tag="PKM23_09955"
     CDS             complement(3939..4985)
                     /locus_tag="PKM23_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91836.1"
                     /translation="MTTVLAQAQEQVTINSQVDRAKILIGDVVRYSLRVSHAPDVTVE
                     MPTPGSNLGMFELRDYQLLPPKKEEGKSILGADYLISTFDTGEFEIPALQVTYTLKGD
                     TARHTLSSEPIRILVQSLNPSEAGDIRDIKPPLVPPRDWRSILLKVGGGVLLIAAAVA
                     AWWYVRRRRQGKSILPERQAPPRPAHEIALEELKALVASDWLATGKHKAWHSELADII
                     RRYIQGRYGIDAPEMTSRQLLGALDKYGLEEKWLPGLKELLSICDMAKFAKYVPEAAE
                     TERLISTAFDFVQGTRLMWAAPEPTQPGEAEADTGAAEENPAGAPAVSAAIAKVGAQG
                     VTTAGAKTENGEGN"
     gene            complement(5123..6058)
                     /locus_tag="PKM23_09960"
     CDS             complement(5123..6058)
                     /locus_tag="PKM23_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91837.1"
                     /translation="MRKRLLLHVVVLCTIVLAGGCGSGKRPAGEAEKVREFAADLVNR
                     SLFSQAIREYESCLRDYDLDANEAANLQYAIGNLYFERLHNYADALAWYLKVKHFHPE
                     SPIVPEVNKKIVACLERLDRSADARQALDETVQLDPSRVQKSRPGAVVARIGSREITL
                     SDLDFEIDQLPPTVREQFRNRETKLQYLREYVATELLYDTARRAGLENDQQVVDGAFQ
                     ARKALMVRRLLQDKVADRINVSEGDIDLYYRAHREDYAEKNDKGKVVREKPLSEVQQQ
                     VAQDLYRDKYQRAFQELIGGMIQSEGVQFFDDQVK"
     gene            complement(6075..>6425)
                     /locus_tag="PKM23_09965"
     CDS             complement(6075..>6425)
                     /locus_tag="PKM23_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNY91838.1"
                     /translation="VVFLISDFLSSGYEKALQIANKRHDIVAISLTDPRELELPDVGF
                     IELEDAETGETWLLDTADEGTRRAFSKRAQQGRGGRQKLFRSMNVDQVEINTRASYVE
                     PLIRFFKMRAKRYR"
BASE COUNT         1119 a         1875 c         2048 g         1383 t
ORIGIN      
        1 ggtcgccgct gcgctggatg ttctcggtca ccttgggttc gtactgctcg aaatcccgcg
       61 gcaggagcac attggggcgg gggatggtct tgatgttgcc ctcgccggag acgaccacct
      121 tgaaggtaac ggcctcattg gtggtcacct ctttcttgtc gatcgaggcc tggatgctga
      181 atttgccggc gagtccgctg aaattggcgg gcttgccggc ttcggggaag gggagcacct
      241 cgatggagac cgggcgcgac tgcaccgtcc tgcgcacggt gcggccgaaa aagggatcat
      301 cgaaaaagct gtcaaaaatg tcgttgctgc gccgccgcgc ctgcacccgg acatcacagt
      361 ccaccccgag gctgccgatc tccaccctgc ccggggaggt cggaaacatc gccattttct
      421 tgaggtcggc cactacatat ttgcggccgt tgatcacctc gttgcgggtc tgcggctgct
      481 ggggcagggg gatctcctcg ctccagaagc cggcggtctc ggggagcttg ctcagggcat
      541 agccggaaac attgacccgc gtatagatcc ggaaggtgac gatcaccggc tcgttctggt
      601 aaacctggcg cttgttgacc agggcgcgga tgaagagatc ctcatccgag gcctcgcttg
      661 ccggcagggg ttgggcctga gtctgaccgg tgacgccctg gccgctccca ctgctgccgc
      721 ttccccccgt cgccgcaccc tggctgacac tcagggggat gggtttgctg ctgatggact
      781 gccccttgta ggtgacgctg accgggggaa tggaaaagct cccctccttg atggtctgat
      841 aaaaataagt gaagctcttc tgcaccgaca tcttgccgtt gataatctgg atgttttgcg
      901 aggtgccgcc ggacccgatc atggccagga attcgctgac atcgggcaga tcgatgcggc
      961 cgacgctgtt ggccccctcg ccggtgagct caacggtcag gacgagctgc tggttgagcg
     1021 ccaggctggt gcggtccacc gacgagctga tcgagagggg ctgggcggca gcggcagcag
     1081 cccagaggat caaggtcaga atatattttc ttgccatcat atgcgtcttc tctctttgca
     1141 aaagctccct ccggcgatca ccagtctttc accaccgtga tccggccggg ggccttcatc
     1201 ttgcggctat ccttctgatt ccgctcatcc tgcttcaagg catccaggat ccgggcggcg
     1261 tcttccttgg aaatctcatc cggccggggc tgcgccttct gttcctgctg ctcctgctgt
     1321 tgctgctctt cctgctgctg ctgttcctgc tgctgttcct gcttgtcctg ctgctcctga
     1381 tcttgctgct gctgctgttg ctgttgttgc tgctgctgtt gctgctgctg gggattctgc
     1441 tgctgctttt gggcctcatc cttcagcttc ttgcgcgcga attcgaggtt gtatttggcg
     1501 tcctcatcag tcggatcgag cttgagcgac tgggtatagg cctgtatggc ctcgggcagc
     1561 ttgttgaggc ggaagagggc gttgcccatg ttgtaatagc tcttggcctg gatgaggcgg
     1621 tcgtcgctcc tgagcgcggc ctgggtctcc ttgagcgcct cctcgtactt gttcttcttg
     1681 tattcggcgg cgccgatatt gaagggggct tccgctctct ccggagcctg ggtttgggca
     1741 tcgcggtatt tgatcaaggc gtcgtcatag cgctcttcag tatagagctt gttgccctgg
     1801 acgatcttgc cgcggccggg ttgggcctga gcccctcccg ctgcgagcag ggcgccggcg
     1861 agaagaaaca atccgatcgg tttcatagga atcaccactt gaaaagacgt ttcagccaga
     1921 aatcgcgccg ctgccgccgc tccgggacaa agatctctgc ggcgagcagg gcgatggcga
     1981 tgatgagcag atattgaaaa cggtcctcga actgggaaaa gcgcatcgag cccagctcct
     2041 tcttctccag cttgtcgaca tcgtcataaa ttttacgcag ctcctcctcg ccgctagtgg
     2101 cgcggaaata cttgccattg gtctggaggg cgatcttttc gagggtcacc tcgtccagtt
     2161 tggtgatcac cacctggcct tcgcggtcct tcttgaatcc cgccgagacc ccatagtcgg
     2221 tcatcaccgg gatgggatct cccttgggag agccgatgcc gacggtgtag atgatcaccc
     2281 cctgacgctc cgcctcctcg gcctgtttca tcacatcgcc ctcatggtcc tccccgtcgg
     2341 tgatgatgac cagcaccttg tgcttgcgct cctgctggtc aaaggcctcg agcgccctgg
     2401 agatggcctg gccgagattg gtgcccggcg tcgggatgag gccgggatcg aggatatcga
     2461 gaaacagctt ggccgcgccg tagtcgaggg tgagggggca ctggacaaag gcagtgccgg
     2521 aaaaggcgat gaggccgacg cggtcgccct ggaggcgatt aagcaggctc tccacctcat
     2581 gcttggcctt ctcgaggcgg ctgggctgaa catcctcggc catcatcgag gtcgagacat
     2641 cgatggcgat catgatgtcg atcccctcgc gcttgacctc ctcgatgcgg gtgccgatct
     2701 ggggccgcgc cagggcgagc agggcgaaga gcagagcgag gaggatcagc gccgccttga
     2761 ggatctgacg ggtcggactg gcgttggccg ccagcttttt catcagctcg ctgctgccga
     2821 agcgctgcag ggcccgggac ttggcgcgga aggcaaagta gtagaagatc agcgccagcg
     2881 gaatcagcca gaggagatgg agaagatatg agttagcaaa tcgcagcata gcgcattccc
     2941 ggaattaggg tattttgcgg aatatcgtgt tgcccaggat cacctcgagg gcgaggagga
     3001 tcagggcggc gatcagatag cgggagaaga gttcgcgata gcgggtgtac tgtttcacct
     3061 cgatcttggt cttttccatc gcgctgatct ctttgtaaat ccgctcaagg ctggtcttgt
     3121 cggtggcgcg gaaatactgg ccgctggtga tctccgccac gcggcggagc agatcctcat
     3181 ctatattgac cggcatgcgc acatagcgcc gtccgaagat ggggtcatcg accgggtaga
     3241 gggcctcccc cttgctgccg gcgccgatgg tgtagacccg gatgttgaag cttttggcga
     3301 tgcgcgccgc ggtgatggga tcgagctcgc cgcggttgtt ctggccgtcg gtgagcagga
     3361 taaccacttt gctccgggcc ttgctgtcgc gcaggcggtt gatgctggtg cccagcccca
     3421 tgccgatggc ggtgccgtcc tcgatctggc cgatatgaat ctcctcgagg aacttgagga
     3481 gaatgccgta atcgagggtg agcgggcact gggtgaagct ctggccggca aagaccacca
     3541 tgccgatgcg gtcattgctc cgtcccttga tgaaatcggc ggcgaccagc ttggccgctt
     3601 ccaggcggtt cttgggttga aaatcctccg ccagcatcga gctggagatg tccattgaga
     3661 ggacgatatc gatgccttcg gtgatcatct cctcctcgcg gctgccggcc tggggccggg
     3721 cgaaagcgat gatcatcagg gcgagggcgg ccaggcgcag cagaaagagg agatgacggt
     3781 agcgccgccg gctggaggtg ccgacctcct tgatgagggc gatgtccgaa tagcgcacgg
     3841 tcgagcggct gcgcaggctg cggcgcaagt accagaagag catcagcggc aggatgagca
     3901 gcaggaagag aaattcagga ttggcaaagc ggaacatatc agttgccctc cccgttttca
     3961 gtctttgctc cagccgtggt cacgccctgc gctccgactt ttgcaatggc ggccgaaacc
     4021 gccggggcgc cggccgggtt ctcctccgca gcgccggtat ctgcttcggc ttcaccgggt
     4081 tgggtcggtt ccggcgcagc ccacatcagc ctggtgccct ggacgaaatc gaaggcggtt
     4141 gagatgaggc gttcggtctc ggcggcctcc ggcacatatt tggcaaactt ggccatgtca
     4201 cagatcgaca gcagctcctt caggccgggc agccacttct cctcgaggcc gtacttgtca
     4261 agggcgccga gcagctgccg gctggtcatt tcgggggcat cgatgccgta gcgcccctgg
     4321 atatagcggc ggataatatc ggccagctcg ctgtgccagg ccttgtgctt gccggtggcg
     4381 agccagtcgc tggcgaccag ggccttgagc tcttcgaggg cgatctcgtg ggccggtctg
     4441 ggcggagcct ggcgttcggg caggatactt ttaccctggc gccggcggcg tacgtaccac
     4501 caggcggcca cagcggcggc gatgagcagc actccgcccc ccaccttgag caggatcgac
     4561 cgccagtcgc gcggcggcac caggggcggc ttgatgtcac ggatatcccc ggcctcgctc
     4621 ggattgaggc tctgcaccag aatcctgatc ggctcgctgc tgagggtgtg ccgcgcggtg
     4681 tcgcccttga gggtgtaggt gacttgcagg gccgggattt cgaattcgcc ggtatcgaaa
     4741 gtggagatga gatagtcggc gccgaggatg gacttgccct cctccttctt cggcggcagg
     4801 agctggtaat cgcgcagctc gaacatgccg aggttgctgc ccggcgtagg catctccacg
     4861 gtgacatcag gggcgtggct gacgcggaga ctgtagcgca ccacatcgcc gatgaggatc
     4921 ttggcgcggt ccacctgcga gttgatggtc acctgctcct gggcctgagc cagtaccgta
     4981 gtcaaaaggc agagaataat cagtaaacgt cgtcttggca tatcgagtga actcagttta
     5041 ttaataacag ttctcacaga ggcacaggga cacagagagc gtgctttcgc tgtgcctccg
     5101 tgccactgtg tggaacaata ttttatttca cctggtcatc gaagaactgc acgccttcac
     5161 tctggatcat accgccgatg agttcctgaa aggcccgctg atatttatcg cggtagaggt
     5221 cctgggccac ctgctgctga acctcgctga ggggcttttc gcgcacgacc ttgcccttgt
     5281 catttttttc ggcataatcc tcccgatggg cacggtaata gaggtcgata tcgccctcac
     5341 tgacgttgat cctgtccgcg accttgtcct ggaggaggcg gcgtaccatc agggccttcc
     5401 tggcctggaa ggcgccgtcg accacctgct gatcgttttc gagtcccgcc cgcctggcgg
     5461 tatcgtaaag cagctcggtg gcgacgtatt cgcgtaaata ctgcagtttg gtctcccgat
     5521 tgcggaattg ttcccgcacg gtcgggggga gctggtcgat ctcgaaatcg aggtcgctca
     5581 gagtgatctc ccggctgccg atgcgcgcca ccaccgcccc cgggcgcgat ttctgcaccc
     5641 gcgagggatc gagctgcacg gtctcgtcga gggcctgacg ggcatcggcg ctgcggtcga
     5701 gccgttccag gcaggcgacg atctttttgt tgacctcggg aacgatcggg ctttcgggat
     5761 ggaaatgctt gaccttgaga taccaggcca gggcgtcggc gtaattgtgc aggcgctcga
     5821 aatagaggtt gccgatggcg tactgcaaat tggccgcctc gttcgcatcg aggtcatagt
     5881 cgcgcaggca gctttcgtac tccctgatgg cctgggaaaa gagcgaacgg ttgaccaggt
     5941 ccgcggcgaa ttcgcgcacc ttttcagcct caccggcggg gcgcttgccc gagccgcagc
     6001 cgccagccag aacaattgtg cataaaacga caacatggag aagcagtctc ttgcgcatgc
     6061 agcacctcat cctcttaacg gtagcgcttg gcccgcatct tgaagaagcg gatcagcggc
     6121 tcgacatatg aagcccgggt gttgatctcc acctggtcca cattcatgct gcggaagagc
     6181 ttctgccggc cgcctctgcc ctgctgggcg cgcttgctga aggcccgccg cgtcccctcg
     6241 tcagcggtgt ccagcagcca ggtttcaccg gtttcggcat cctccagctc gataaacccg
     6301 acatccggca gttccaactc gcgcggatcg gtcagggaga tggcgacgat atcgtggcgc
     6361 ttgttggcga tctgcaaagc cttttcatat ccggaggaga gaaaatcgga gatgagaaag
     6421 accac
//
LOCUS       DAOPWU010000189         6378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12120_length_6378_cov_3.464653, whole genome shotgun sequence.
ACCESSION   DAOPWU010000189 DAOPWU010000000
VERSION     DAOPWU010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12120_length_6378_cov_3.464653"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PKM23_09970"
     CDS             complement(<1..587)
                     /locus_tag="PKM23_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNY91839.1"
                     /translation="MIVETFVTGPLEENCYLVRSSQGEEAALIDPGDDGEELLAELTA
                     RGVRLTRILLTHAHFDHVGAVALLQQASGAQVLLHEGEKSILAGLPLQSALFGLSAPE
                     PFQVSTWLKGGEKIAIGSLELQVLATPGHSAGGISFYGHGHLFPGDVLFRSSIGRSDL
                     PGGDGALLLHSIRTRLFSLPDETIVHPGHGEETTIG"
     gene            complement(601..1107)
                     /locus_tag="PKM23_09975"
     CDS             complement(601..1107)
                     /locus_tag="PKM23_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91840.1"
                     /translation="MRYLKLLTLALLPPLLLQAQSWSLKRTAFVTAGGRVASGRYTAV
                     AAAGIPTLSGMESASFSVGPVTAVELAEGQRPEEFALLQNYPNPFNQSTTLSWQLPQA
                     GEVRIALYTLRGEEAALLYAGRQQAGIYRLAWAGQDGRGRPLPSGVYFLRFTAGAHQQ
                     VKKLTLVR"
     gene            complement(1126..3348)
                     /locus_tag="PKM23_09980"
     CDS             complement(1126..3348)
                     /locus_tag="PKM23_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91841.1"
                     /translation="MKRRAFSLFCALFALLTLTAAGAQELPPSTAADTTAVPRTDLAL
                     QKSEAAVTSTVVAWQGFLTDNNGNPVTGILPARLSIWSEAGSGTKLWQEEHNLDVYQG
                     YFTAELGRTIELPRTVFEGSPRWIQLEVNGETLTPRKELLNVPFSLYAANAGALGQVA
                     ADQFYTRSQANSSANNEIDAAKLGGKKAVDFYDRFQLDAGYVARGSTNSVTGEMVVDG
                     SIQRSDIGFNLGAGTITAVTPGNGLSGGGSAGAVSLALNTEYATGQAYDTRFVRREES
                     AVVSSGMLRDGSVTSAKIADNSIQPADLGFALGTITSVVAGNGLTGGGVTGPVTLSLN
                     SNYQNGSAYDARFVRKGEETSITSAMIKDGEVSGADIKNFSITQEDIGFNLGDITSVQ
                     TMNGLMGGGITGDLTLQLEAAYRDGSAYDSRFIQRGQVGGITGPMILDGTIEPRDLAF
                     SAGDITSVTGASGVTTTSSGQSGDVVIRLEEGYLSGAAYDTRFISKNLAGGVTSYNIE
                     DMGVQSTDLADNIIQQRHFGNTFSMSKNYIGGAVFNVANLAGSNSWGIQGSGLTGGVR
                     GSSSTGIGVLGSGALYGVHARLEGTTSINSFALFVEGQARCTTGGWADLAEKINGSEE
                     LEAGDVVVIDPGSRYAVRRCREAGDTRVAGIISTNPTILVGTLVSGGGYPLALSGIVP
                     CKVTAANGAIQPGDLLTTSEVAGHAMKAVEIRPGTIVGKALEGLESGTGLIQVLATLH
                     "
     gene            complement(3555..4730)
                     /locus_tag="PKM23_09985"
     CDS             complement(3555..4730)
                     /locus_tag="PKM23_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNY91842.1"
                     /translation="MVNEKIDQAIAIMREQGVDLWLTFVRETTSSPDPVLDLLAGAHV
                     VWPSAFVLTASGKAVAIVGSLDMHGVRDAASRYDVKGYVDSVAGPLLELLEEEQPKRI
                     AINYSANDVMADGLSHGLYLILVDYLKGSPWGERLESSEALVAALRGRKSPAELSRIK
                     SAIRETLDIYDQVTAHVRAGMTEREVAAFITDLMEARGLTPAWSADHCPSVFTGPDTA
                     GAHAGPTGRRIEPGHIMNTDFGVRYEGYVSDLQRTWYFLRPGESEAPAEVKRGFATIH
                     GAIQAAAAALKPGVEGWTIDKVARQYIIDAGYDEFPHALGHQVGRSAHDGAGLLCPVW
                     DRYKNLPFLKVEIGQVYTLEPRLTVPGHGVVTIEEMVVVTETGCEFLSEPQRDLLVV"
     gene            complement(4845..5789)
                     /locus_tag="PKM23_09990"
     CDS             complement(4845..5789)
                     /locus_tag="PKM23_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNY91843.1"
                     /translation="MVGFIIIILFMAFIALMILRNNPATAGAANAAVSGLRRFGLPLV
                     GLLILLLLISQSVVVIQAGTAGVVKRLGAVRGEMAPGLHFILPFVDQVVLYPTVKKTY
                     EASEIPEQSQADYPDIIISALTSDGQQIKVGITARFMIQPGKAAWILQNLGTERDYVE
                     KVVKTEIRGSGRRVPTKFAAYDLYTKTSYEAQQVLFDEISPKFQENGLILDELVLRNI
                     TFTDEYARTLEEKQIALENISTERNKLEQEKMRKEQKIVAAEGDARSIEIRQAALTKN
                     PTIIQWEFVQKLAPNIQWGILPQGAVPMLNMQGFGAKQ"
     gene            complement(5824..>6378)
                     /locus_tag="PKM23_09995"
     CDS             complement(5824..>6378)
                     /locus_tag="PKM23_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91844.1"
                     /translation="MDAGEAAVASFQGMADPLVPFGSAKSPDFGFVIHGSAPITRTAQ
                     RLGITTALAGIPDMGHGVPDQAMMDSLIRFERNFLYLLISTQVGVERLGQGQPNSYGL
                     LQSYPNPFNAATTIRYLLPHESEIALAVHAVDGRRVALLEQGRRSAGEHTVHWQAGDL
                     ATGVYIVSLHADGLVLSQRVTLIK"
BASE COUNT         1097 a         1956 c         2170 g         1155 t
ORIGIN      
        1 cctatggtgg tctcctcgcc gtgcccggga tggacgatgg tctcgtccgg cagggagaag
       61 agtctggtgc ggatggagtg cagcagcaga gcaccatcac cgcccggcag atcggagcgg
      121 ccgatggaac tgcggaagag gacatccccg gggaaaagat ggccgtggcc gtaaaacgaa
      181 atcccgccgg ccgagtggcc gggggtggca aggacctgca gctcgaggct gccgatagcg
      241 attttctccc cgcccttcag ccaggtggag acctgaaagg gttcgggcgc ggagaggcca
      301 aagagggcgc tctgcagggg cagcccggcg aggatgctct tctccccctc atggagcagc
      361 acctgcgccc cgctcgcctg ctgcagcagc gcgaccgcgc caacatggtc gaaatgggcg
      421 tgggtcagca ggatgcgggt aagacgcacc ccccgcgccg tcagctccgc caggagttcc
      481 tccccgtcgt cgccgggatc gatgagggcg gcctcttctc cttgagaaga gcgcaccagg
      541 tagcagtttt cctcgagggg gccggtgaca aaggtctcga cgatcatgcc tgccggcctc
      601 ctagcgcacc agggtgagtt tcttcacctg ctgatgggcg ccggcggtga agcggaggaa
      661 atagaccccg ctcggcaggg gacgcccgcg gccatcctgc ccggcccagg cgaggcggta
      721 gatccccgcc tgctgacggc cggcgtagag cagcgccgcc tcctccccgc gcagggtgta
      781 gagggcgatg cgcacctccc cggcctgggg cagctgccag ctcagggtgg tgctctggtt
      841 gaagggattg ggataattct gcagcagggc aaactcttca ggccgctgcc cctccgccag
      901 ttccacagcg gtgaccggac cgacactgaa gctggcgctc tccatgccgc tcagggtggg
      961 aatgcccgcc gccgcaaccg cggtgtagcg gccggaggcg accctgcccc cggcggtgac
     1021 aaaggcggtg cgcttgaggc tccaggactg ggcctgcaga agcaggggcg gcagcagcgc
     1081 cagggtgagc agtttaaggt atctcatagt tctcctcagg gccttctagt gcagggtcgc
     1141 cagaacctgg atgaggccgg tgccgctctc gagcccctcg agggccttgc ccacgatcgt
     1201 ccccggcctg atctcgacgg ccttcatggc atggccggcc acctccgagg tggtgagcag
     1261 gtcgccgggc tgaatagctc cgttggcggc ggtcaccttg cagggcacga tgccgctgag
     1321 ggcgagggga tagccgccgc cgctcaccag ggtgccgacc aggatggtcg gattggtcga
     1381 gatgatcccc gccacgcggg tatctccggc ctcacggcag cgccgcaccg catagcggga
     1441 gccgggatcg atgacgacca cgtcgccagc ctccagttcc tcggagccgt tgatcttttc
     1501 ggccagatcg gcccaaccgc cggtggtgca gcgcgcctgg ccctcgacga aaagggcgaa
     1561 gctgttgatc gaggtcgtgc cctcgaggcg ggcgtggacg ccatagagag ccccggagcc
     1621 gagcacaccg atgccggtag agctggagcc gcgcaccccc ccggtcagtc cgctcccctg
     1681 aatgccccag ctgttggatc ccgcgaggtt ggcgacatta aaaaccgccc ccccgatata
     1741 attcttgctc atgctgaagg tgttgccgaa atggcgctgc tggatgatgt tgtcggcgag
     1801 gtcggtggac tggaccccca tatcctcgat attgtagctg gtcacgccgc cggcgagatt
     1861 cttgctgata aaacgggtgt cgtaggcggc cccggagaga tagccctcct cgaggcggat
     1921 gacgacgtcc ccgctctgcc cggaggaggt ggtggtgact ccgctcgcgc cggtgacgct
     1981 ggtgatgtcg ccggcggaga aagcgaggtc gcgcggctcg atggtgccgt caaggatcat
     2041 gggaccggtg atgccgccca cctggccccg ctggatgaag cggctgtcgt aggcagagcc
     2101 gtcgcgatag gccgcctcga gctgcagggt gaggtcgccg gtgatgccgc cgcccatgag
     2161 gccgttcatg gtctgcaccg aggtgatgtc gcccagattg aagccgatgt cctcctgggt
     2221 gatcgaaaaa ttcttgatgt cggcgcccga gacctcgccg tccttgatca tcgcgctggt
     2281 gatgctggtc tcctcgccct tgcgcacgaa gcgggcgtcg taggcgctgc cgttctggta
     2341 attgctgttg agggagaggg tgaccggacc ggtgaccccg ccgccggtga ggccgttgcc
     2401 ggccacgacc gaggtgatgg tgccgagggc aaagccaagg tcggcgggct ggatggagtt
     2461 gtcggcgatc ttggccgaag tgacgctgcc gtcgcgcagc atgcccgaac tcaccacagc
     2521 actctcctcg cggcggacga aacgggtgtc gtaggcctgg ccggtggcgt actcggtgtt
     2581 gagggcgagg gagacggcgc cggcgctgcc cccgccgctg aggccgttgc ctggggtgac
     2641 ggcggtgatg gtgcctgcac cgaggttgaa gccgatatct gagcgctgga tcgatccgtc
     2701 cacaaccatc tcgccggtga cactgttggt gctgccgcgc gccacatagc cggcatcgag
     2761 ctggaagcgg tcgtagaaat ccaccgcctt tttgccgccc agtttggcgg cgtcgatctc
     2821 gttattggcg gagctgttgg cctggctgcg ggtgtagaac tgatccgcgg ccacctggcc
     2881 gagggccccg gcgttggcgg catagaggct gaagggcaca ttgagcagct ccttgcgcgg
     2941 ggtcagggtc tcgccgttga cctcgagctg gatccagcgc ggactgccct cgaagacggt
     3001 gcgcgggagt tcgatcgtgc gccccagctc ggcggtgaaa taaccctgat agacgtcgag
     3061 gttgtgctcc tcctgccaca gcttggtccc gctccccgcc tccgaccaga tgctcagccg
     3121 cgccggcagg atgccggtga cgggattgcc gttgttgtcg gtgagaaagc cctgccaggc
     3181 gaccacggtc gaggtcaccg ccgcctcgct cttctgcaag gcgagatcgg tgcggggcac
     3241 ggcagtggtg tccgccgcgg tagagggcgg gagctcctgg gctccggctg cggtcagggt
     3301 gagtaatgcg aatagggcgc aaaagaggga aaaagcgcgg cgcttcatga tgccggtacc
     3361 tccagttgaa ctttccggga tgcttggcga tggatttact ttaataataa taggtgcttt
     3421 gcgccgcatt tgcagaatat tttcgccgcc gcgggtcaaa taaaaacccc ggcgggccgg
     3481 gtggcgcgcc ggggcaagtc ggagaatggc atcacaattc tgcgggtgga gcgagcccac
     3541 tgctgccgag ggtttcagac caccagcagg tcgcgctgcg gctccgagag aaattcgcag
     3601 ccggtttcgg tcaccaccac catctcctcg atggtcacca cgccgtggcc gggcacggtc
     3661 aggcgcggct ccagggtgta gacctgtccg atctcaacct tgagaaaggg gaggttcttg
     3721 tagcggtccc agaccgggca aagcagcccg gccccgtcgt gggcgctgcg gccgacctga
     3781 tggccgaggg catggggaaa ctcgtcatac cccgcgtcga tgatgtactg ccgcgccacc
     3841 ttgtcgatgg tccatccctc cactccgggc ttgagggccg cagcagccgc ctggatcgct
     3901 ccatggatag tcgcgaaacc gcgtttgacc tcggccgggg cctcgctctc gcccgggcgc
     3961 aggaaatacc aggtgcgctg caggtcagag acatagccct cgtagcgcac cccgaaatcg
     4021 gtgttcatga tgtggccggg ctcgatgcgc cgcccggtcg gcccggcgtg ggccccggcg
     4081 gtgtccggac cggtaaagac cgagggacag tggtccgccg accaggccgg ggtaaggccg
     4141 cgggcctcca ttaaatcggt gatgaaggcg gccacctccc gttcagtcat gccggcgcgg
     4201 acgtgggcgg tgacctggtc atagatgtcg agggtctcgc ggatagccga tttgatccgg
     4261 ctcagctcgg ccggggattt gcgtccgcgc agggccgcca ccagggcctc gctcgactcg
     4321 agccgctccc cccaggggct gcctttaaga tagtcgacga ggatgagata gaggccgtgc
     4381 gaaagcccgt cggccatgac gtcgttggcc gaatagttga tggcgatgcg cttcggctgc
     4441 tcctcctcga gcagctcgag cagcggtccg gccaccgaat cgacatatcc cttgacgtca
     4501 tagcggctcg ccgcatcgcg caccccgtgc atgtcgaggc tgccgacaat ggcgacggcc
     4561 ttgcccgagg cggtgaggac aaaagcggag ggccagacca catgcgcccc ggcgagcaga
     4621 tcgaggaccg gatcgggtga agaggtggtc tcgcgcacaa aggtgagcca gagatccacg
     4681 ccctgctctc tcatgatggc tatggcctgg tcgatctttt cattgaccat gtatttcgtt
     4741 ctccagatct gattggggaa tccccgtgca ggcgcgattt ccggcctcct gccccgcatc
     4801 ggggccggaa ggagcccagg cccgcagccg cgcggggatg gtgattactg tttggccccg
     4861 aatccctgca tgttgagcat tggcaccgcc ccctgcggca ggatccccca ctggatgttc
     4921 ggggccagct tttgcacaaa ctcccactgg atgatagtcg ggttcttggt cagggccgcc
     4981 tggcggatct cgatgctgcg ggcatcgccc tcggcggcga cgatcttttg ctccttgcgc
     5041 atcttttcct gttcgagctt gttgcgctcg gtgctgatgt tctccagggc gatctgcttc
     5101 tcctcgagag tgcgggcata ttcgtcggtg aaggtgatgt tgcgcagcac cagctcgtcg
     5161 aggatcaggc cgttctcctg aaacttgggt gagatttcgt caaaaaggac ctgctgggct
     5221 tcatagctgg tcttggtgta gaggtcatag gcggcgaact tggtcggcac gcgccggccc
     5281 gaaccgcgga tctcggtttt gaccaccttt tcgacatagt cgcgctccgt gcccaggttc
     5341 tgcaggatcc aggccgcctt gccgggctga atcatgaagc gggcggtgat gccgaccttg
     5401 atctgctggc cgtcggaggt gagggccgag atgatgatgt cgggatagtc ggcctggctc
     5461 tgctccggga tctccgaggc ctcataggtc tttttcactg tcgggtagag cacgacctgg
     5521 tccacaaagg gcaggataaa atgcagacct ggagccatct ctcccctgac cgcgccgagg
     5581 cgcttgacca caccggcggt gccggcctgg atcaccacca ctgactggga gatgagcagc
     5641 agcaggatca gaaggccgac aagcggcagg ccgaagcgtc ttagcccgct cacagcggcg
     5701 ttggccgccc cggcggtggc gggattgttg cgcagaatca tcagggcgat gaacgccatg
     5761 aagagtatta tgatgatgaa accgaccatg gcatacctcc tcttcaggat ggtagagaat
     5821 cacctatttg attaatgtca ctcgctgtga caatacaagg ccatcggcat gaagcgagac
     5881 gatataaaca cccgtggcca aatcacctgc ctgccaatgc acggtatgtt cgcccgcact
     5941 ccggcggcct tgttccagca gtgcaacccg gcggccgtcc acagcatgca ccgcgagtgc
     6001 gatctcgctc tcatgaggca agagatagcg gatggttgtg gcggcgttga agggattggg
     6061 atagctttgc agcagcccgt atgagttcgg ctgtccctgc ccgaggcgct cgacgccgac
     6121 ctgagtggaa atcagcaggt agagaaaatt gcgctcaaac cggatgaggc tgtccatcat
     6181 cgcctgatcg ggtaccccgt gccccatgtc ggggatgccg gcgagggcgg tggtgatgcc
     6241 gaggcgctgg gcggtgcggg tgatcggcgc gctgccatgg atgacaaagc cgaaatccgg
     6301 ggatttggcg ctcccgaatg gcaccagggg atcggccatt ccctgaaaac tggcaacagc
     6361 agcttcgccg gcatccat
//
LOCUS       DAOPWU010000190         6319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12273_length_6319_cov_5.598340, whole genome shotgun sequence.
ACCESSION   DAOPWU010000190 DAOPWU010000000
VERSION     DAOPWU010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12273_length_6319_cov_5.598340"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="PKM23_10000"
     CDS             <1..1567
                     /locus_tag="PKM23_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY91845.1"
                     /translation="EACRGFFTALRMDESRRPLRVKILPRDRVSPASAEVCGFSRDLI
                     ASTNATWLFDLPPADRGARVRFALPLYPQPQRPVTEWTLPVYPQIDELACVELAADTL
                     ASRMINAALLEAPARPLAAAPEALWRQNPVLAFRAAWRAHQGLASANGPGKAALQADC
                     TTLYRLAGAGAGQKPLQAWSLLHLGALLNQSQRSSEAIEPLLEAETLFGPMANPAGLI
                     LSRRELAASYTERTEWPMARQTLNRLLNIPPDSLTQAFTLERIAQLYEKEGRTAEAVQ
                     AWHRTAWMNLAIGRPYESAMVYQRLGQLMRETGDLERSVAYLDTFLTQAGAMASEPAL
                     ARAHFQMGLTRLARAERETALNHFLKAGDYLEMLGDHSGMARADLNIGAIYWQLGDTL
                     KARQRYFAVINQAASSGDSVMVLMSAVNLADIHLTQKNYEQAQAFFDRALAIAQQARN
                     SREQAKITYAKGLAYLKEGRLRTGYARIKEAMALGGGSVNGDPQKEQAFLDKLSLLIG
                     DIENLRGAAPPRP"
     gene            1530..2330
                     /locus_tag="PKM23_10005"
     CDS             1530..2330
                     /locus_tag="PKM23_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91846.1"
                     /translation="MKTCAALRRRGRSGPRRRMAGRSRLPASTRALAARMDKAVREYR
                     MLRAGDRLLVAVSGGADSMSLLHLLSRRLPVYAPGARLRAVYVDMGFGEGNEERCRIM
                     RAFWRQLGVSGRILRTTIGPLAHSEANHENPCFLCSRIRRKQIFATAELFDCNRIAFG
                     HHKDDLVETLLLNMVFGREISTSPPNLALHGGRYHLLRPMLFAEEELVKAYAADNAIP
                     LFAQECPSDGTSRRQTIKEWLAALEAEHPGSRENIFQSMRRVKPDYLL"
     gene            2456..3325
                     /locus_tag="PKM23_10010"
     CDS             2456..3325
                     /locus_tag="PKM23_10010"
                     /inference="COORDINATES: protein motif:HMM:NF018378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HNY91847.1"
                     /translation="MRLKLSYPFDWQGLEDFQSYDYDSAGLPRVDYGGEEGLRYNPIT
                     IAQYGLHSLQLFGREEREEYRTRALACAEWLAAQARAYDQGMYAWIYDFSLPFYGPRA
                     PWISAMAQGEAVSLLVRCHVLEHRSDFLDLAHGAIRLLDLPVSEGGTLGRLSDGSPWF
                     EEYPTEPASHVLNGHFFALLGAADWADHTDLPDAWQRCERGVASIEKNWRLWDRGYWT
                     RYDLHPTNRLASRMYQKVHVRLMRLLAFRTARPVFYKAARRWQAMLYDPWCNLLWLAV
                     KSREKIRLRGYGQ"
     gene            3322..4650
                     /locus_tag="PKM23_10015"
     CDS             3322..4650
                     /locus_tag="PKM23_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY91848.1"
                     /translation="MKKVLMLSYYFPPLGMGGTQRAAKFARYLPEFGWEPTVLTVKPI
                     SYWAEDSSLLEEMAATRIVRTGSLDPQRLLHLLTRRAGKETGAAAGREGAGTSGGTAA
                     GQEKAVTFGGAAAWLNRKLLPWFLLPDSKILWRWHATRAAARLLRSGEFSAIWSTSPP
                     HSTHLIARRLAHRFRLPWVADFRDEWAGGVVVHEPTPWHRRRQQALQGKVARAAAALI
                     GVTPGIVAGLEAAGGSGRCHLITNGFDAADLATIGEAAMNREEAHFTLCHCGSISRFS
                     NPAPLLEALIPGDARWLRLRFIGYDANGGFEEQVRRRGLEEMISWRGYLPHREALVSM
                     AAADALLLIACDAPKARFIPGKTFEYLASRKPILAISNVEETIRLLEEMPGVILCAPD
                     QPAAIRTALLRLQSEPELLRAAAERNVQHYDRRDQAGELARILDRITEER"
     gene            4657..>6319
                     /locus_tag="PKM23_10020"
     CDS             4657..>6319
                     /locus_tag="PKM23_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNY91849.1"
                     /translation="MNKRPLASLFALFACLSGLPAAAGGQADYAWPTDASRMLTSTFG
                     EYRSHRFHAGMDVKTFGQVGYKALAVRPGSVSRVEVSPWGYGRVLYLLLDTGETAVYG
                     HLQKFALAIAARVREEQQRTGRYAVSLRFEKGEIPVAQDELIGWTGQSGSGAPHLHFE
                     LRDAEESPLNPLLKGYQVKDAIPPSIRKVMVIPLNATARVDGDLEPLILTPRAAQRGR
                     MQIDRTIPLFGRVAFAVSMLDQIDGAANRMNVHSYRLLIDDREIFSARCDHYPYQLDH
                     QADLDRDYRALVREGDLFQRLFREIGNELPFYAGDKPWYGALECDPASGAANWLTGIT
                     RALGFFWDLPSGVTILERGPHTLRIEARDFNGNLSIVEGRLQSGPPPEPAADPQGVDP
                     AQASYTISAAYYDSYVRAALSASRPLRRLPLLTARYGDGHEEEIVLTRGGRNLLLGGV
                     PLLPNRPGPVRLRIASPQDEKPLHDAMLNFITVRKGRDKKVTTQDGLCELSFAEETLF
                     KPLFVRANTRNRGAEKLPLVSRIYEIEPADVPMSSFVTVLIRTEPG"
BASE COUNT         1030 a         2384 c         1864 g         1041 t
ORIGIN      
        1 ggaagcctgc cgcggctttt tcaccgccct gcgcatggac gaaagccggc ggccgctgcg
       61 ggtcaagatc ctgccgcgcg atcgggtcag ccccgccagc gcggaggtct gcggtttttc
      121 ccgggatctc atcgcctcca ccaatgccac ctggctcttc gacctgccgc ccgcggacag
      181 gggcgcccgg gtgcgcttcg ccctgccgct ctacccccag ccccagcgtc ccgtcaccga
      241 atggaccctg ccggtttatc cccagatcga cgagctcgcc tgcgtcgagc tggccgccga
      301 caccctggcc tcgcggatga tcaacgccgc actgctcgag gcccctgccc gtcccctcgc
      361 cgccgcgccc gaggccctct ggcggcagaa tccggtgctc gccttccggg ctgcctggcg
      421 cgcccatcag gggctcgcca gcgccaatgg cccgggcaaa gccgccctcc aggccgactg
      481 cacaaccctt tatcgtctgg ccggcgcggg agccggacaa aaacccctgc aggcctggtc
      541 gctcctccat ctcggtgcgc tgctcaacca gagccagcgc agcagcgagg cgattgaacc
      601 cctgcttgag gccgagaccc tcttcggccc gatggccaat ccggcgggat tgatcctcag
      661 ccggcgcgaa ctggccgcct cctacaccga gcgcacggaa tggccgatgg ccaggcagac
      721 cctcaaccgt ctgctcaaca tccccccgga cagcctcacc caggccttca ccctggaaag
      781 gatcgcccag ctctatgaga aggagggccg gaccgccgag gcggtgcagg cctggcatcg
      841 caccgcctgg atgaatctcg ccatcggccg cccctatgag tcggccatgg tctatcagcg
      901 cctcggccag ctcatgcgtg agaccggtga cctcgagcgc tcggtggcct atctcgacac
      961 ctttctcacc caggccggcg ccatggccag cgagcccgcc ctcgcccgcg cccatttcca
     1021 gatggggctg acccgcctcg cccgcgccga gcgcgagacc gccctcaacc attttctcaa
     1081 ggccggcgac tatctggaga tgctcggcga ccacagcggc atggcccgcg ccgacctcaa
     1141 tatcggcgcc atctactggc agctcggcga taccctcaag gcccgccagc gctatttcgc
     1201 ggtcatcaac caggccgcct cgagcgggga ttcggtcatg gtcctgatga gcgcggtcaa
     1261 cctcgccgat attcacctca cccagaagaa ctatgagcag gcgcaggcct ttttcgaccg
     1321 cgccctcgcc attgcccagc aggccaggaa ttcccgggaa caggccaaga tcacctacgc
     1381 caaggggctc gcctatctca aggaggggcg gctgcggacc ggctacgccc gcatcaagga
     1441 ggcgatggcc ctcggcggcg gctcggtcaa cggcgacccc cagaaggagc aggcctttct
     1501 cgacaagctc tccctgctca tcggcgacat tgaaaacctg cgcggcgctg cgccgccgcg
     1561 gccgtagcgg gcctcgccgc cgcatggccg gccgcagccg cctccccgcc tccacccgcg
     1621 ccctcgccgc gcgcatggac aaggccgtcc gcgaataccg catgctgcgg gccggcgacc
     1681 gcctcctcgt cgccgtctcc ggcggggccg actccatgtc ccttcttcac ctcctcagcc
     1741 gccgtttgcc tgtctacgcc ccgggcgccc gcctgcgcgc cgtctatgtc gacatgggtt
     1801 tcggagaggg gaacgaggag cgctgccgga tcatgcgcgc cttctggcgg cagctcgggg
     1861 tgagcggccg catcctccgc accaccatcg gcccgctcgc ccacagcgag gccaaccacg
     1921 agaatccctg cttcctctgc tcccgcatcc gccgcaaaca gatctttgcc actgcggagc
     1981 tttttgactg caacaggatc gccttcggcc accacaagga cgacctggtc gaaactcttc
     2041 tgctcaacat ggtcttcggc cgcgagatca gcaccagccc gcccaacctc gcccttcacg
     2101 gcggccgcta tcacctgctg cgccccatgc tcttcgccga agaggagctg gtcaaggcct
     2161 atgccgccga taacgccatc ccgctcttcg cgcaggagtg ccccagcgac gggacctccc
     2221 gccgccagac catcaaggag tggctcgccg ccctcgaggc cgaacatccc ggcagccgcg
     2281 agaacatctt tcagtcgatg cggcgggtca aacccgacta cctgctttag aacgccgccc
     2341 ccccgcgctt cctccttgca attcccgcta aaatttatta ccttttgacg ccggccggcg
     2401 cctgtgtccg cccgggcctg ctccaccatc attccgtgaa ggaaacaagt tctgcatgcg
     2461 gctcaagctg agctacccct ttgactggca gggattggag gatttccaat cctacgacta
     2521 tgattccgcc ggactgccgc gcgtggatta cggcggcgaa gaggggctgc gctataatcc
     2581 catcaccatc gcccagtatg ggctccattc ccttcagctc ttcggcaggg aggagaggga
     2641 ggaatatcgc acccgcgccc tcgcctgcgc ggagtggctg gcggcacagg ccagggctta
     2701 cgaccagggc atgtacgcct ggatttacga tttctcgctc cccttttacg gcccgcgggc
     2761 gccgtggatc tcggccatgg cccaggggga ggcggtctcc ctgctggtgc gctgccacgt
     2821 cctcgaacac cgcagcgatt tcctcgatct cgcccatggc gccatccgcc tgctcgacct
     2881 gccggtctcc gaggggggaa ccctcggccg gctctccgat ggctcgccct ggttcgagga
     2941 gtacccgacc gagccggcct cgcacgtctt aaacggtcac tttttcgccc tgctcggggc
     3001 cgcggactgg gccgaccata cggacctgcc cgacgcctgg cagcgctgcg aacgcggtgt
     3061 ggccagcatc gagaaaaact ggcggctctg ggaccgcggc tactggaccc gctacgatct
     3121 ccatcccacc aaccgcctcg cctcgcgcat gtaccagaag gtccatgtgc ggctgatgcg
     3181 gctgctcgcc ttccgcaccg cccgcccggt tttttacaaa gccgcacggc gctggcaggc
     3241 tatgctctac gacccctggt gcaacctgct ctggctggcg gtcaagagcc gggagaagat
     3301 ccggctgcgg gggtacgggc agtgaaaaaa gtgctgatgc tcagctatta tttccccccg
     3361 ctcggcatgg gcgggaccca gcgtgcggcc aagttcgccc gctacctgcc cgaattcggc
     3421 tgggagccga cggtcctcac cgtcaagccc atctcctact gggcggagga ctcttcgctg
     3481 ctggaggaaa tggccgctac ccgcattgtg cgcaccggtt cgctcgatcc ccagcgcctg
     3541 ctccatctgc tcacgaggcg ggcagggaaa gaaaccggtg ctgcggccgg acgcgaagga
     3601 gccggcactt cggggggaac agcagccgga caggaaaagg cggtcacttt cggcggagcg
     3661 gcggcctggc tgaaccgcaa gctgctcccc tggttcctcc tgcccgacag caagatcctc
     3721 tggcgctggc atgccacccg ggcggcggcc cggctgctgc gctcgggaga gttcagcgcg
     3781 atctggtcga cctcgccgcc ccattccacc catctcatcg ccaggcggct ggcgcaccgc
     3841 ttccgcctcc cctgggtggc tgatttccgc gacgagtggg ccggaggggt ggtcgtccac
     3901 gagcccacgc cctggcaccg ccgccgccaa caggctctgc aaggaaaagt ggcgcgcgcg
     3961 gccgcagccc tgatcggggt cacgcccggc attgtcgccg ggctcgaggc cgccggcgga
     4021 agcggccggt gccatctcat caccaacggc ttcgatgccg ccgatctggc caccatcggc
     4081 gaggccgcca tgaaccggga ggaggcgcat tttaccctct gccactgcgg ctcgatcagc
     4141 cgcttcagca atcccgcccc cctcctcgag gccctcatcc ccggggatgc ccgctggctg
     4201 cgcctccgct tcatcggcta tgacgccaac gggggattcg aagagcaggt gcgcaggcgg
     4261 gggctggagg agatgatctc ctggcggggc tatctgcccc accgcgaagc cctcgtgagc
     4321 atggccgccg ccgatgccct gctgctgatc gcctgcgacg cccccaaggc ccgcttcatc
     4381 ccgggcaaga cctttgagta tctggccagc cgcaagccca tcctggcgat cagcaacgtc
     4441 gaggagacca tccgcctgct cgaggagatg ccgggggtca tcctctgcgc gccggatcag
     4501 ccggccgcca tccgcaccgc cctgctccgc ctgcagagcg aacccgaact gctcagggcc
     4561 gcagcggagc gaaatgtaca acattacgat cgcagagatc aggccggcga actcgcccgg
     4621 attctggacc gcatcaccga ggaacgataa atccacttga acaagagacc gctagcatcc
     4681 cttttcgccc ttttcgcctg cctctccggc ctgcctgccg ctgccggcgg gcaggccgat
     4741 tatgcctggc ccaccgacgc cagccgcatg ctgacctcga cctttggcga ataccgcagc
     4801 caccgcttcc acgccggcat ggacgtcaag accttcggcc aggtgggcta caaggccctg
     4861 gcggtgcggc ccggatcggt ctcccgcgtc gaggtctcgc cctggggtta cggccgcgtc
     4921 ctctatcttc tcctcgatac cggcgagacc gcggtttacg gccatctgca gaaattcgcc
     4981 ctcgccatcg ccgcccgggt gcgcgaagag cagcagcgca ccggacgcta cgccgtcagc
     5041 ctccgcttcg aaaagggcga aatccccgtg gcgcaggatg aactgatcgg ctggaccggc
     5101 cagagcggtt ccggcgcgcc tcaccttcat tttgagctgc gcgacgccga agaatcaccc
     5161 ctcaatcccc tgctcaaggg ttatcaggtc aaggatgcca ttcccccctc gatccgcaag
     5221 gtgatggtga tcccgctcaa cgccaccgcg cgcgtcgacg gcgacctcga acccctcatt
     5281 ctcacgccca gggcggccca gcgggggcgg atgcagatcg accgcaccat cccgctcttc
     5341 ggacgggtcg ccttcgccgt cagcatgctc gaccagatcg acggcgccgc caaccgcatg
     5401 aacgtccaca gctaccgcct gctcatcgat gaccgggaga tcttttccgc ccgctgcgac
     5461 cattacccct accagctcga ccaccaggcc gacctcgacc gcgattatcg cgccctggtg
     5521 cgcgaggggg atctcttcca gcgcctcttc cgcgagatcg gcaacgaact gcccttctat
     5581 gcgggcgaca agccctggta cggggccctc gagtgcgatc ccgcctcagg cgccgccaac
     5641 tggttaaccg gtatcacccg cgccctcggc ttcttctggg acctcccctc aggcgtcacc
     5701 atcctcgaac gagggcccca caccctccgc atcgaggcgc gcgatttcaa tggcaatctc
     5761 agcatcgtcg agggccgcct ccagtccggg ccgccccctg aaccggccgc cgacccgcag
     5821 ggtgttgatc cggcacaggc ctcctatacc atctccgccg cctactatga ctcttatgtc
     5881 cgcgctgcgc tctcggcctc ccgccccctg cgccgcctcc cccttctcac tgcgcgctat
     5941 ggtgacggtc acgaggagga gatcgtcctc acccgcggcg gcaggaacct cctgctcggg
     6001 ggcgtgccgc tgctgccgaa ccgccccgga ccggtccggc tccgcatcgc gagcccgcag
     6061 gatgaaaagc ccctccatga tgccatgctc aactttatca cggtgcgcaa ggggagggac
     6121 aagaaggtca ccacccagga cggcctctgt gagctctcct tcgcggaaga aaccctcttc
     6181 aagcccctct ttgtccgcgc caacacccgc aaccgcggcg cggaaaaact tcccctggtg
     6241 agccggatct acgagatcga gccggccgat gtgccgatga gcagctttgt cacagtgctg
     6301 atccgcaccg agcccggtc
//
LOCUS       DAOPWU010000191         6290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12354_length_6290_cov_3.718043, whole genome shotgun sequence.
ACCESSION   DAOPWU010000191 DAOPWU010000000
VERSION     DAOPWU010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12354_length_6290_cov_3.718043"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKM23_10025"
     CDS             complement(<1..412)
                     /locus_tag="PKM23_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNY91850.1"
                     /translation="MENNLYDQREFLKKIGLSAAELAEWEKNGLIRSEVRIDSNIPCY
                     SEEHVVEAQQIQHLIQIGYSLPEIRKILKKIGLPGPEARKQARQATEYLTVGELAEQA
                     RINNRTIKYWEERGIITPDRRTSGGFRLYGKHYVQ"
     gene            complement(551..1477)
                     /gene="queG"
                     /locus_tag="PKM23_10030"
     CDS             complement(551..1477)
                     /gene="queG"
                     /locus_tag="PKM23_10030"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNY91851.1"
                     /translation="MDLKSVILERAFSLGFDRIGFARAEALPGHALREWLEKGCNAEM
                     AWMAARLPMRLDPRVMLPGARTVIVAAANYFTTGESSGDPRLACLARYALGDDYHHVL
                     KDRLKELLVFIRTLAPGVQARICVDAAPIWEKEWARRAGIGWRGKHSLIIAPELGSWI
                     LLGEIVTSLDLEPDQPIPDRCGSCTLCLEACPTGALIAPCTLDARRCIAYHTIESGDA
                     IPEAVRRAMGNRIFGCDICQSVCPWNLRWAVPADAPSFHPRPHWRNAPLAELVHWPEE
                     RFTAAAHRSPLQRRGYAGFIRNVEAALANLHE"
     gene            complement(1565..4105)
                     /locus_tag="PKM23_10035"
     CDS             complement(1565..4105)
                     /locus_tag="PKM23_10035"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNY91852.1"
                     /translation="MSAIGKYLSDTAAEKIRQAIDEAGGNEVFFIGYTEEDLIVHDVA
                     VAARGHKTAVPVITQLAAEADVVIHNHPSGHLTPSEADLSIAAGLDPFSVAFYIVDNQ
                     AERIYVVVEPFAKREIVPLDEESLVNLVQAGGPVSRELSGYEVRPQQEEMIRAVTEAF
                     NREKIATIEAGTGTGKTLAYLIPAIRWALQNKERVVVSTNTINLQEQLIRKDIPFLQK
                     VLPDEFTAVLVKGRGNYACLRKVNDLAGELDLQSDEEEQEELKQLIDWARGSSDGSKA
                     DLNYIPREQVWEKIGSESDTCTRSRCPFFRDCFVNKARRHAARANILVVNHHLLFADL
                     SIRYQIGSVSEAAVLPPYSRIIFDEAHHLEDVATNYFGDRVTRAGIMRILSRLHRQQK
                     QLFKGHLHTVLYKLRRKRGQIDEHLLVKIEESVTGRLVPEVAALHEATQVLMDHIFEA
                     VKGFAQENQGFEIKQRLVPAVVQSLLVDSGLSRLLQEYTSSMLELSAGLAALTGLLNK
                     LQSGADDDFASVVIEIKASSDRLAAAAAVIGEVVFKQDEESIRWIEVRPGYKGRHVVR
                     LQCSPLDIAPMMQKAVYDTYDTIVMTSATLTVENSFEFLGKRVGLNALDPGRRLELQL
                     PAPFDYERQAVVCIPTDIPDPNDRAFSAELGKLIFRAVSISNGRAFVLFTSYGLLTII
                     YRQLEDSFRKLGINPLRQGDENRHELLARFRRDKASVLFATDSFWEGVDVEGEALENV
                     IISKLPFKVPNEPVIEARWEAIERAGGNPFMEYAVPLAVLKFKQGFGRLIRRKSDRGS
                     VIVFDNRVVRKSYGKRFMRSLPPCRTVIGGQEQVFAELGSFFGRRKTR"
     gene            complement(4102..5043)
                     /locus_tag="PKM23_10040"
     CDS             complement(4102..5043)
                     /locus_tag="PKM23_10040"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HNY91853.1"
                     /translation="MFTYDQKGIQIAGAELWLDALRKVSFSFVSHGHADHLKNHLRIL
                     ATPATATFHTLRAKKAELLPLNFGESLELDGMRIRLYPAGHILGSAMIHIERDGTSLL
                     YSGDFKLRPSATCEPIDVPHADILITESTYGHPEYVVEQSRDALIEELLGFISDCRRG
                     GYTPVVMAYTLGKSQEAMKILGDRGVSVRVHPAIWQMAEVYARFGVAFDRCAPWQGAA
                     IAPEEVLIVPPHLAFTRALQFVPRRKTVFLSGWTHSGHGPGWRTDHRIPLSDHADFNE
                     LIEFVGMVRPHKIYTTHGFAGFPDHLRAAGWDAEMLA"
     gene            5249..>6290
                     /locus_tag="PKM23_10045"
     CDS             5249..>6290
                     /locus_tag="PKM23_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91854.1"
                     /translation="MQVRTNHYSFSCQAGLLAILLTLLPLAARTVRITVTPSDSSAFI
                     ADLQPLFGLPWQLAATPEAAAHFDCESSFIDQGRGFLLPLYPEIQPLCELWHYEAGRW
                     RRENLGAYSDRAAYTLFALSPRDIWLSFHPGRTLKQDLLHFDGRGWRQVPSPNTDRIR
                     TIYMLSPTSGWAGCEWGQIMHYDGREWRLVPSPTEAHVLFMVMTSDSSGFAWTKTHRK
                     ENAPLIYNGREWRRAPGDSTGLLQLLAAVPAINHGTSHWPIGILTRFNRLQRPAAIVD
                     TLLLEAPLAPKRLLFCHRSGTLSSGHLARSATVSGGGWIAHLTEKEEQDSSHVWQTIS
                     FFTLAGDKRSLVI"
BASE COUNT         1163 a         1937 c         1992 g         1198 t
ORIGIN      
        1 tctggacata atgcttgccg tagagcctga agccgccgct ggtgcggcgg tcgggggtga
       61 tgatcccccg ctcttcccag tacttgatgg tgcggttgtt gatccgggcc tgctcggcca
      121 gctcccccac agtgaggtac tcggtcgcct gcctggcctg cttccgggcc tccggcccgg
      181 gcaggccgat cttcttgagg atcttgcgga tttcgggcag ggagtagccg atctgaatga
      241 gatgttggat ctgctgcgcc tccaccacat gttcctcgct gtagcagggg atgttgctgt
      301 cgatgcgcac ctcgctccgg atcaggccgt tcttctccca ctccgccagc tccgccgcgg
      361 acaagccgat ctttttaagg aattcccgct ggtcatagag gttgttttcc atatacgttc
      421 cttagcgtat agtttattaa taaaatatca gtatacgtat acattatact gctattccaa
      481 aacaaatgca agcagaattt tcagtaacat cagaagtagt cggatggagt cgcagcgaag
      541 gaaggcaagc tcactcgtgc agattggcca gggcggcctc gacattgcgg atgaatccgg
      601 catacccgcg ccgctgcagg ggagagcggt gcgcggctgc ggtgaaacgc tcctccggcc
      661 aatggaccag ctcggccagg ggggcattcc gccagtgggg acggggatga aaggagggag
      721 catcggccgg cacagcccaa cggagattcc aggggcagac cgactggcag atatcgcagc
      781 cgaaaatgcg gttgcccatc gcccggcgca ccgcctcagg gatggcgtct ccggattcga
      841 tggtgtggta ggcgatacag cgcctggcgt cgagggtgca gggggcgatg agggcgccgg
      901 tcgggcaggc ttcaaggcag agggtgcagg agccgcagcg gtcgggaatg ggctggtccg
      961 gttcgagatc gaggctggtg acaatctcac caagcaggat ccacgacccc agttcgggtg
     1021 cgatgatcag gctgtgcttg ccgcgccagc cgatgccggc acggcgcgcc cactccttct
     1081 cccagatcgg agcggcatcg acacagatcc gggcttgcac cccgggggcc agggtgcgaa
     1141 tgaaaacaag cagctccttc agccggtctt tcagcacgtg gtgataatca tcgccgaggg
     1201 cgtagcgggc gaggcaggcg aggcgaggat cgcctgagct ctcccctgtg gtaaagtaat
     1261 tggccgccgc gacaatcacc gtgcgcgccc ccggcagcat cacacgcgga tcgaggcgca
     1321 tcggcaggcg ggcggccatc cacgccatct cagcgttgca gcccttttcc agccattccc
     1381 gaagggcatg accgggcagc gcctccgccc gggcgaatcc gatacggtca aagccgagag
     1441 agaaagcacg ttccagtatg accgatttca gatccatcgc gatctttacc ctgcctgagg
     1501 cggctttgtt ccagaagcga gagcggcgcc gtcgagtctg ccggggcgcc acactgccac
     1561 ggagtcagcg agtttttctg cgcccaaaaa agctgcccag ctcggcgaat acctgctcct
     1621 ggccgccgat caccgtccgg caggggggca gggagcgcat gaaacgcttg ccatagctct
     1681 tgcggaccac gcggttgtcg aagacgatca ccgagccgcg gtcgctcttg cgccggatga
     1741 ggcgtccgaa accctgtttg aacttgagta ccgccagggg gacggcatac tccatgaagg
     1801 ggttgccgcc ggcgcgctcg atcgcctccc agcgcgcctc gatcaccggc tcgttgggca
     1861 ccttgaaggg gagcttgctg atgatgacat tctccagggc ctcgccctcg acgtcgaccc
     1921 cctcccagaa gctgtcggtg gcgaagagca ccgaggcctt gtcgcggcga aagcgcgcca
     1981 gcagctcatg gcggttttcg tcgccctggc gcaggggatt gatgcccagc ttgcggaaag
     2041 agtcctcgag ctggcgatag atgatggtga gcaggccgta ggaggtgaag aggacaaagg
     2101 cccggccgtt ggagatggag accgcgcgga agatcagctt gcccagctcg gccgaaaaag
     2161 cccggtcgtt cggatccggg atgtcggtgg ggatgcagac cacggcctga cgctcatagt
     2221 cgaagggagc gggcagctgg agctcgagac gccggccggg atcgagggcg ttcagtccga
     2281 cgcgcttgcc gagaaattcg aaggaatttt cgacggtaag ggtcgcggag gtcatcacga
     2341 tggtatcata ggtatcgtag accgcctttt gcatcatcgg cgcgatatcc aggggcgagc
     2401 attgcaggcg gacgacatgg cgccccttgt agccgggtcg gacttcgatc cagcggatgc
     2461 tctcctcatc ctgtttgaag accacctctc cgatcactgc agctgcggcg gccagacggt
     2521 cggaggaggc cttgatctca atcaccaccg aggcgaaatc atcatcagcc ccactctgca
     2581 gcttgttcag cagtcccgtc agagcggcga gtcctgccga aagctcgagc atgctcgagg
     2641 tgtactcctg gagcagacgg ctcaagccgc tgtcgacgag caacgactgc accaccgcgg
     2701 gcaccaggcg ctgcttgatc tcgaagccct ggttctcctg ggcgaatccc ttgaccgcct
     2761 cgaagatgtg gtccatcagc acctgggtgg cctcatgcag cgcggccacc tccggcacca
     2821 gccggcccgt tacgctctcc tcgattttca caagcagatg ctcatcgatc tggccgcgtt
     2881 tgcgccgcag tttgtagagc acggtgtgga gatggccctt gaagagctgc ttctgctggc
     2941 ggtgcaggcg gctgaggatg cgcatgatgc cggcgcgggt gacgcgatcg ccgaaatagt
     3001 tggtggccac atcctcgagg tgatgggcct cgtcgaagat gatgcgggaa tagggcggca
     3061 gtaccgcggc ctcgctgacc gacccgattt ggtagcggat cgagaggtcg gcgaaaagga
     3121 gatgatggtt caccaccagg atgttggcgc gggcggcgtg gcgccgcgcc ttgttgacaa
     3181 aacagtcgcg gaagaaggga cagcggctgc gggtgcaggt gtcgctctcc gagccgatct
     3241 tctcccagac ctgctcgcgc gggatgtaat tgagatcggc cttgctgccg tcgctggagc
     3301 cgcgcgccca gtcgatcagc tgcttgagct cctcctgctc ctcctcgtcg ctctgcagat
     3361 cgagttcgcc ggcgaggtcg ttcaccttgc gcaggcaggc ataattgccc ctccccttga
     3421 ccagcaccgc ggtgaactcg tccggcagca ccttctgcag gaaaggaata tccttccgga
     3481 tcagctgctc ctgcagattg atggtgttgg tcgagaccac cacccgctcc ttgttctgca
     3541 gggcccagcg gatcgccggg atgagatagg cgagggtctt gcccgtgccg gtgcccgcct
     3601 cgatggtggc gatcttttcc cggttgaagg cctcggtcac tgcgcggatc atctcctcct
     3661 gctggggccg gacctcgtat ccggagagct cgcgcgagac cggtcctccc gcctgcacca
     3721 ggttgaccag gctctcctca tcgagaggga cgatctcccg cttggcgaag ggctccacca
     3781 ccacatagat ccgctcggcc tggttgtcga cgatatagaa agcgacgctg aagggatcaa
     3841 ggcccgcggc gatggagaga tcggcctcgg aaggggtgag atggcctgag ggatgattgt
     3901 ggatgaccac atcggcctcg gcggcgagct gggtgatgac cggcacggcc gtcttgtggc
     3961 cgcgcgccgc cacggcgaca tcgtggacga tcagatcctc ctcggtgtag ccgatgaaaa
     4021 agacctcgtt gccccccgcc tcatcgatgg cctggcggat cttttcggcc gccgtatcgg
     4081 agaggtattt gccaatggcg ctcatgccag catctccgca tcccagccgg ccgcgcgcag
     4141 atggtcaggg aagccggcga aaccgtgggt ggtatagatc ttgtgcgggc gtaccatgcc
     4201 gacaaattcg atcagctcat tgaaatcggc gtgatcgctg agcgggatgc ggtggtctgt
     4261 gcgccagccc gggccatggc cgctgtgcgt ccagccggag agaaagacgg ttttgcgccg
     4321 ggggacaaac tgcagggcgc gcgtgaaggc gaggtggggc gggacgatga gcacctcctc
     4381 gggagcgatg gcggccccct gccagggggc gcagcggtca aaggcgacac cgaagcgggc
     4441 atagacctcg gccatctgcc agatcgcggg atggacacgc acgctgacgc cgcggtcgcc
     4501 gaggatcttc atcgcctcct gggacttgcc cagggtatag gccatcacca ccggggtata
     4561 gccgccgcgg cggcagtcgc tgataaagcc gagcagctct tcgatcagcg cgtcgcggct
     4621 ttgctcgacc acatattcag gatggccgta ggtgctctcg gtgatgagga tatcggcatg
     4681 agggacatcg atcggctcgc aggtggcgct gggacgcagc ttgaaatccc cgctgtaaag
     4741 cagggaggtg ccgtcacgct cgatatgaat catcgccgaa cccaggatat ggccggccgg
     4801 atagaggcgg atgcgcatcc catccagctc cagcgactca ccaaagttga gcggcagcag
     4861 ttccgctttt ttcgcccgca gggtatgaaa ggtggccgtc gccggggtgg cgaggatacg
     4921 gaggtggttt ttcagatggt cggcgtggcc gtgcgagacg aaggaaaagc tcaccttgcg
     4981 cagggcgtcg agccaaagct ctgcgcccgc gatctgaatg cctttttgat cgtaggtaaa
     5041 catgggattt ccaatgaagg ggttgatgca aaaataggga gaaaatatga cgattccaat
     5101 cctttttcag ggattctcca gccgccatgc ctggccgtca tggctgattt agattgacaa
     5161 aatagtaaaa tttggctatt ttggggcata ctcttgcacc ccactctgct gctcacggaa
     5221 acgggctcat cgggccgggc gatcccgcat gcaggtcaga acgaatcatt actccttttc
     5281 ttgccaggca ggcttgctgg ccattctcct caccttgctt ccccttgccg ccaggaccgt
     5341 tcgtataacg gtcacccctt ccgattccag cgccttcatc gccgatctgc agcccctctt
     5401 cggcctgcca tggcagctgg ccgccacgcc cgaggctgcc gcccatttcg actgcgaatc
     5461 ctcgtttatt gatcaagggc gcggctttct tctaccctta tatcctgaga tccagccgct
     5521 ttgtgaactc tggcattacg aagcgggccg ctggcgccgg gaaaacctgg gtgcatattc
     5581 ggaccgcgcc gcttacaccc tctttgcgct ctctccccgg gatatctggc tcagctttca
     5641 ccccggacgc accctcaagc aggatctgct ccatttcgac ggccgcggct ggcgccaggt
     5701 cccgtcgccc aacacggacc gcatccgcac catctatatg ctctccccta cctccggctg
     5761 ggccggctgt gaatgggggc agatcatgca ctatgacggc cgcgaatggc ggctggtccc
     5821 ctccccgacc gaagcacacg tcctcttcat ggtcatgacc agcgacagca gcggcttcgc
     5881 ctggaccaag acccatcgca aggagaacgc tccgttaatc tataatggcc gggaatggcg
     5941 ccgggcccct ggcgattcga ccgggctgct gcagctgctg gctgctgtac ccgccatcaa
     6001 ccatggcacc agccattggc ccatcgggat cctgacgcgc ttcaaccggc tgcagcggcc
     6061 ggcggctatc gtggacaccc tgctgctcga ggccccgctt gcccccaagc gcctgctctt
     6121 ctgccatcgc agcgggaccc tctcctccgg acacctcgct cgctctgcaa cagtttccgg
     6181 cgggggctgg atcgcgcatc tcaccgaaaa ggaggaacag gatagcagcc atgtctggca
     6241 gaccatctcc tttttcaccc tcgccggcga caaacgcagc ctggtcatcc
//
LOCUS       DAOPWU010000192         6275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12393_length_6275_cov_4.128778, whole genome shotgun sequence.
ACCESSION   DAOPWU010000192 DAOPWU010000000
VERSION     DAOPWU010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12393_length_6275_cov_4.128778"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..>1086)
                     /locus_tag="PKM23_10050"
     CDS             complement(188..>1086)
                     /locus_tag="PKM23_10050"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNY91855.1"
                     /translation="GCALILSLLAGLLLTCSTPADQAYKEEIDGWHARRVANLTKPDG
                     WLSLAGRFWLEEGENRFGSDAGNPVRFPEGRAPARMGSFFLEKGVVRVRILPEVLVTA
                     ADSSRVQETIMQDDAGGSPTILRYGTLSWHVIRRSERFAIRLRDSAHENLKNFQGIER
                     YPVDPDWRLKAKFVPYEPAKKLRIVNVVGQVEEQACPGALLFTLRGVEYRLDVLDEGP
                     REPFFVIIADETSGEETYGGGRFLYVARPDSAGETIIDFNKAYNPPCSFTPYATCPLP
                     PEQNRLPIAIRAGEKRYKPKTN"
     assembly_gap    1087..1186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1187..2947
                     /locus_tag="PKM23_10055"
     CDS             <1187..2947
                     /locus_tag="PKM23_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY91856.1"
                     /translation="LMLLTPLIMRDLVDKTIPAKDLHRLLILALSLLIIPVVSGAIGV
                     SQRRLNARVGEGVICDLRMVLYANLQRMSLRFFTNTRVGELMSRLNNDVMGAQTAISS
                     TIVGIVTQLIQAVAVLAVMLSLEWRLTLISVIIMPLFILAARLLGNKLRDIARSQMEA
                     NAQMNAMMNETLNIGGALLVKLFGRRQLEVDRFGQRAAQVRDLGVKRAFTGSIFMAII
                     GLISAVGTALVYGLGGYFVILGAFTIGTIVAFGSYLHQLYGALQQLSNAPVEFATSMV
                     SFERVFEVIDLPVDIPEIEGARTMQEVKGEIRFEEVYFSYNNTDENLLSDVRRYGSMD
                     NVTGVLSGKMASKDDDDAPRSQARANALEAITFCAEPGQIVAIVGPSGAGKTTLTYLI
                     PRLYDPTSGVVRIDGHDLREVTLESLSRQIGMVTQETHLFHDTIRTNLQYAKLDAAQE
                     ELEEACRIANIHDFIREMPDGYDTIVGERGFRLSGGEKQRLALARVILKNPRILLLDE
                     ATSSLDSKSEALIQDALEKVMADRTSVVIAHRLSTILAADLILVMDRGRIVESGSHDT
                     LLAQGGLYTTLYETQFRKDD"
     assembly_gap    3042..3141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3142..3333
                     /locus_tag="PKM23_10060"
     CDS             <3142..3333
                     /locus_tag="PKM23_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNY91857.1"
                     /translation="LAATDLGLGTCWIAAFDPQAAREVLQLPESAVPVAFTPLGYPAD
                     QPGPKKRKTLEEIVKKEHW"
     gene            complement(3314..5587)
                     /locus_tag="PKM23_10065"
     CDS             complement(3314..5587)
                     /locus_tag="PKM23_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNY91858.1"
                     /translation="MTGATFGIGYELAKLFAHDGDQLVLVARSQARMTAVAEELAALG
                     AAAVVPLVKDLADPAVPEEIFQAVTEMGLEIDVLINNAGFGDSGPYIHTVWKTNAEMV
                     QVNITSLLHLTRLFLPGMAARSSGRILNVASTAAFQPGPDMALYYATKAFVLSFSEAL
                     AEECRGMGVTVTALCPGPTATEFQVRANTEGTGIGQSKRGPLRMMSAAEVAKVGYRGV
                     LTGRRVVIPGAFNRVGAWSGRHLPRRLIMKTIKQLHRKRATATLLLAALLAGLAPMAR
                     AADEPPAIAREMRGVWVATVANIDWPSRPGLPVEEQQAEAVAILDRVEAMRMNTVVLQ
                     VRPQADALYPSELEPWSCYLTGAQGQAPEPFYDPLHFWIEQAHARGIELHAWLNPYRA
                     GHPAMRSDYAENSVVRRHPEWVHALADTGYYWMDPALQGVQEHSLAVVLDLLHRYDLD
                     GIHFDDYFYPYREYNLGRDFPDEVAWQAYQAGGGKMARDDWRRDAVNRFIQRLYKEIK
                     KTKPWVKFGISPFGIYRPGYPAGYGGGFDQYAVLYADARLWFNKGWIDYYTPQIYWNI
                     ASMQTSFPVILGWWEGENTKGRHLWPGLYLRPGVAHEEMAREIIGQVMVTRGMIQQAP
                     GTIQFSMRSLMNPDSVIFRALAGGPFARAALMPAFPWLDKKAPEPPVVQAAPDSSGWR
                     ISWQPKGKETPFVYVLYLQRAGSWLPEIYPAGQHSVQLPVEAGKVTAVAVTAVDRCGN
                     ESKKKVISLEEITNAPF"
     gene            complement(5646..>6275)
                     /locus_tag="PKM23_10070"
     CDS             complement(5646..>6275)
                     /locus_tag="PKM23_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91859.1"
                     /translation="PVSRQRQKSGWTLLLSVLLACAPQSSRESFIQAAVTSRPVMEVQ
                     DLYKLLYQARCGIGHLIASRASARDYLLAEIAALPAAGAEAAAGAGENLLESCAPDGK
                     MVRLNLRPFVQRGLDPEKLLDAMLESARLTRPDTAALRADWAAAGALIRSGRLPFAKE
                     SYAQFTRENLSAGFPVMHHSAAYTAAYHPAYRVLRLDAFRRYFPAVTAL"
BASE COUNT         1120 a         1934 c         1941 g         1080 t
ORIGIN      
        1 cctgctgact acctggtcag aaccgatctg gtcacgctgt ttgatgccag cccgcccacg
       61 gccggcgtga agcccgccac gcgctactgg accgggtccg cctgggctga ccccggcacc
      121 gtgatcgacg ggaacctgct ggaaccggcg gttcctcgcc ggtaattcga ccggggccaa
      181 ggcccggtca gttcgtcttt ggcttgtagc gcttctcacc ggcgcggatg gcgatgggca
      241 gacggttctg ctccggcggc aggggacagg tcgcgtaggg ggtgaaggag caggggggat
      301 tgtaggcctt gttgaaatcg atgatggtct cacccgcgga atcgggccgg gcaacataga
      361 gaaagcggcc gccgccatag gtctcttcgc cgctggtctc gtcggcgatg atgacgaaga
      421 agggctcgcg agggccctcg tcaaggacat cgaggcggta ctccacgccc cggagggtga
      481 agagcagggc gccgggacaa gcctgctctt ccacctgccc gaccacattg acgatcctga
      541 gcttcttcgc cggctcatag gggacaaatt tcgccttgag gcgccagtcg ggatcaaccg
      601 gatagcgttc gatgccctga aagttcttca gattttcgtg cgcgctgtcg cgcaggcgaa
      661 tggcgaagcg ttcgctgcgc cggatgacat gccaggagag ggtcccgtat cgaaggatgg
      721 tgggggagcc gccggcatca tcctgcatga tcgtctcctg cacccggctg ctgtcggcgg
      781 cggtgaccag aacctcgggc agaatgcgga cgcgcacgac gcccttttcg agaaaaaagg
      841 agcccatccg ggccggagcg cgcccctcag ggaaacgcac cggattcccg gcatcgctgc
      901 caaagcggtt ctccccctcc tccagccaga aacgccccgc cagactcagc cagccgtcgg
      961 gtttggtgag attggccaca cgccgggcgt gccagccatc gatttcctct ttgtaagcct
     1021 ggtccgccgg cgtcgagcag gtgagcagca atcccgccag cagggaaaga atcagagcac
     1081 agcctcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctga tgctgctcac
     1201 cccgctgatc atgcgcgacc tcgtcgacaa gaccatcccc gccaaggatc tccaccgtct
     1261 gctcatcctg gccctgagcc tgctgattat cccggtggtt tcgggggcga tcggggtctc
     1321 gcagcggcgc ctcaatgccc gcgtgggcga gggggtgatc tgcgacctgc gcatggtgct
     1381 ttatgccaac ctgcagcgca tgtccctgcg cttcttcacc aacacccggg tcggcgagtt
     1441 gatgagccgg ttgaacaacg atgtcatggg ggcgcagacg gcgatcagca gcacgatcgt
     1501 cggcatcgtc acccagctca ttcaggccgt ggcggtgttg gcggtgatgc tctccctgga
     1561 atggcgactg accctgatca gcgtcatcat catgcccctc ttcatcctcg ccgcccggct
     1621 gcttggcaac aagctgcgcg acatcgctcg cagccagatg gaggccaatg cccagatgaa
     1681 tgcgatgatg aacgaaaccc tcaacatcgg cggcgcgctt ctggtcaagc tcttcggacg
     1741 ccgccagctc gaggttgacc gcttcggcca gcgtgcggca caggtgcgcg atctgggggt
     1801 caaacgggct ttcaccggtt cgatctttat ggccatcatc ggtctgatca gcgccgtcgg
     1861 cactgccctg gtttacggcc tgggcggcta tttcgtcatc ctcggcgcct tcaccatcgg
     1921 cacgatcgtg gccttcggct cctacctgca ccagctttac ggagcgctgc agcagctttc
     1981 caacgccccg gtcgagttcg ccacttcgat ggtcagcttt gagcgcgttt tcgaggtcat
     2041 cgatctgccg gtcgacatcc cggagatcga gggtgcccgg accatgcagg aggtcaaggg
     2101 cgagatccgc ttcgaggagg tctatttcag ctataacaat acggatgaaa acctgctcag
     2161 cgatgtgcgc cgctacggca gcatggacaa tgtgacggga gtgctctccg gcaagatggc
     2221 gagcaaggat gacgatgacg ccccgcgcag ccaggcgcgc gccaacgccc tggaggcgat
     2281 caccttctgc gcggaaccgg ggcagatcgt ggccatagta ggtcccagcg gcgccggcaa
     2341 gaccacgctc acctatctca tcccccggct gtacgatccc acctcagggg tggtccgcat
     2401 cgatggtcat gatttgcgcg aggtgacgct cgaatccctc tcccggcaga tcggcatggt
     2461 cacccaggag acccatctct ttcatgacac catccgcacc aatctgcagt acgccaagct
     2521 ggatgctgcg caggaggagc tggaggaggc ctgccggatc gccaacattc acgactttat
     2581 ccgggagatg cccgacggct acgacaccat cgtcggagag cggggcttcc ggctcagcgg
     2641 cggggagaaa cagcgcctcg ccctggcgcg ggtgattctg aagaatccgc gcatcctgct
     2701 gcttgatgaa gccaccagca gcctcgacag caaatccgag gccctgatcc aggacgccct
     2761 ggagaaggtg atggccgacc gcaccagcgt cgtcatcgcc caccggctga gcaccattct
     2821 ggcggccgat ctgatcctgg tcatggaccg cggccgcatt gtcgagagcg gcagccacga
     2881 caccctgttg gcgcagggcg ggctttacac cactctctac gagacccagt tccgcaagga
     2941 tgattaaatc aggtggaagg agaggttcag agcatggaat tacgacaggt gatggaatcc
     3001 cgctacagcg tacgcgccta caagccgaat ccggtggagc cnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nctggccgcg accgatctcg gactgggcac ctgctggatt
     3181 gccgctttcg atccccaggc cgcgcgtgag gtgctgcagc tgccggagag cgccgttccg
     3241 gtcgccttca ccccgcttgg ctatcccgct gaccagcctg ggcccaaaaa acgcaagact
     3301 ctggaagaaa tcgtcaaaaa ggagcattgg tgatctcttc cagagagatc accttctttt
     3361 tgctttcgtt gccgcagcgg tccacggcgg tgacggcgac cgcagtcacc ttgccggctt
     3421 cgaccggcag ctgcacactg tgctgtccgg ccggataaat ctccggcagc caactccccg
     3481 cgcgctggag gtagagcaca tagacgaagg gggtctcttt ccccttcggc tgccaggaga
     3541 tgcgccagcc gctgctgtcc ggagcggcct gcaccacggg aggctcaggg gcctttttgt
     3601 cgagccaggg gaaggcgggc atcagcgccg ctctggcgaa cggccccccg gccagtgccc
     3661 tgaagatgac gctgtccgga ttcatcaatg agcgcatgct gaactggatg gtccccggcg
     3721 cctgctggat catgccgcgg gtcaccatca cctggccgat gatctcgcgg gccatctcct
     3781 cgtgcgccac ccccggccgc agatagaggc caggccacaa atgacgcccc tttgtattct
     3841 ccccttccca ccatcccagg atcaccggaa agctggtctg catgctggcg atgttccagt
     3901 agatctgcgg ggtatagtaa tcgatccaac ccttgttgaa ccacagccgg gcatccgcat
     3961 agagaacagc gtactggtcg aagcctccgc cgtagcccgc aggatagccc gggcggtaga
     4021 tgccgaaggg gctgatgccg aatttgaccc acggtttggt cttttttatc tccttgtaga
     4081 gccgctggat gaagcggttg acggcgtcgc gccgccagtc gtcccgcgcc atttttcctc
     4141 cgccagcctg ataggcctgc caggccacct catcgggaaa atcccgcccc agattatact
     4201 cccggtaggg atagaaataa tcatcgaaat gaatgccgtc aagatcatag cggtggagga
     4261 gatccagcac cacagcgagt gaatgctcct gcaccccctg gagagcggga tccatccagt
     4321 aatagccggt gtcggcgagg gcgtgcaccc actccgggtg gcgccgcacg accgaattct
     4381 ccgcataatc ggagcgcatc gccggatggc cggcgcggta gggattgagc caggcgtgca
     4441 gttcgatgcc gcgggcatgg gcctgctcga tccagaagtg gaggggatca taaaagggct
     4501 cgggggcctg tccctgcgcg ccggtcagat aacaggacca gggctccagc tccgaggggt
     4561 agagtgcgtc cgcctgcggg cgcacctgca ggacgacggt gttcatgcgc atggcttcca
     4621 cccggtcgag gatcgccaca gcttcggcct gctgctcctc caccggcagg ccgggacgcg
     4681 atggccagtc aatgttggcc acagtagcca cccagacgcc gcgcatctcg cgtgcaatgg
     4741 ccggcggctc gtctgccgcc cgggccatcg gtgccagacc cgccagcagc gccgccagca
     4801 gcagagtggc ggtggcgcgc ttgcgatgca gctgtttgat ggttttcatg atcagccggc
     4861 gcggcagatg gcggccgctc caggcgccga cgcggttgaa ggcgccgggg atgaccaccc
     4921 tccggccggt cagtaccccg cggtacccga ctttagcgac ctccgcggcg ctcatcatcc
     4981 tgagcggtcc gcgcttcgac tggccgatgc ctgtgccttc ggtattggca cgcacctgaa
     5041 actccgtcgc cgtcggaccc gggcagaggg cggtgacggt cacccccatg ccccggcact
     5101 cctccgcgag ggcctcgctg aaggagagca caaaggcctt ggtggcgtaa tagagcgcca
     5161 tgtcgggtcc gggctgaaag gccgccgtgg aggcgacatt gaggatgcgc ccgctactgc
     5221 gtgcggccat cccgggcaga aagagccggg tcagatgcag caggctggtg atattgacct
     5281 gaaccatctc cgcattggtt ttccagaccg tgtgaatgta cgggcctgag tcgccgaaac
     5341 cggcattgtt gatcagcaca tcgatctcga ggcccatctc cgtcaccgcc tgaaagatct
     5401 cttccggcac ggcggggtca gccagatcct tgaccagagg gacaacggca gcggcgccca
     5461 gggcggccag ttcctcagcg accgcggtca tacgggcctg gctgcgggcg accagcacca
     5521 gctgatcccc gtcgtgggcg aagagcttgg ccagttcata gccgatgccg aaggtggcgc
     5581 cggtgatcag gagagttctt ttcttctcga tctttcttcc ttgcaggttt ggtgttgagg
     5641 ggggcctaaa gggccgtcac cgccgggaaa tagcgccgaa aggcgtcgag ccggagcacc
     5701 ctgtaggcgg gatgataggc ggcggtataa gccgcagagt ggtgcatgac gggaaatccc
     5761 gcagagagat tctcccgggt aaattgtgca tagctctcct tagcgaaggg gaggcggccg
     5821 gagcggatca gtgccccggc ggcggcccaa tcggcgcgca gggccgcggt atcgggtcgg
     5881 gtgaggcgcg cgctttccag catcgcatcg agaagttttt ccggatcgag accgcgctgc
     5941 acgaaggggc gcagattgag gcgcaccatt ttgccgtcgg gcgcacagga ttcaagcaga
     6001 ttttcgccgg cgcccgccgc agcctccgcc ccggcggcgg gaagagcagc gatctccgcc
     6061 agcagataat cccgagccga ggcgcgcgag gcgatgagat ggccgatgcc gcagcgggcc
     6121 tgatagagca gcttgtagag atcctgcacc tccatcaccg gccggctggt cacagcagcc
     6181 tggataaagc tttcccgcga agactgcggc gcgcaggcca gcagaacgga taacaacagg
     6241 gtccagccgg atttttgccg ctggcggctc acagg
//
LOCUS       DAOPWU010000193         6270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12405_length_6270_cov_4.836846, whole genome shotgun sequence.
ACCESSION   DAOPWU010000193 DAOPWU010000000
VERSION     DAOPWU010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12405_length_6270_cov_4.836846"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..691
                     /locus_tag="PKM23_10075"
     CDS             116..691
                     /locus_tag="PKM23_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY91860.1"
                     /translation="MLFDHFNLIARFYDRAIGPHEAEKLGAQLALPENGRLLDVAGGT
                     GRVLSGLASWRGSMVLCDYAMGMLRQAQAKKVALPARSAAEQMPFADGRFERVLMVDA
                     LHHVADQPQTLREMYRVLSPGGRMVIEEFDIRHWAIRIVALLEKIALMRSHFMPLSEI
                     AGILQKAGAHVEIRMDGAARAWIVAEKPPAS"
     gene            complement(786..1916)
                     /locus_tag="PKM23_10080"
     CDS             complement(786..1916)
                     /locus_tag="PKM23_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNY91861.1"
                     /translation="MKIVRVALLLFLLLLAVLVLCPRKAISEQPPRVQLALLLDTSNS
                     MDGLIDQAKSQLWKIVNEFIPARVDGRRPEIAVALYHYGSPELGERTGYVRQLTPLTA
                     DLDKVSDLLFGLTTHGGDEYCGTVIGQAVEELQWGTGTNDYRAIFIAGNEPFTQGRVD
                     FRTSCKKAIAKGILVNTIFCGDHQEGIETSWKEGADLADGSYFNIDQNARSIDPPTPF
                     DQELADLGAELNKTYVAYGQAGAAGKASQERQDLNAAKASPASLVQRAITKSTAIYSN
                     SGWDLVDAVQQKEVRLAELKKEELPEEMRSLPAAERQKYLDQKYAQRKAIQEKMDGLR
                     VKRAAFIAEEQNKASQSGEVNTLDKAMSKALRTQAEKANYTF"
     gene            complement(1953..4214)
                     /locus_tag="PKM23_10085"
     CDS             complement(1953..4214)
                     /locus_tag="PKM23_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HNY91862.1"
                     /translation="MRRLFTAFLLVLVFTGPAALLADGMIIIPRLPRPPFPPHFPPAL
                     APLEVRYHHVSVKIEEQVAVTEIDQIFYNPHPHELEGTYLFPIPRGGQLDQFSMEIDG
                     RSVEAELLDAGKARRLYEEIVRARRDPALLEYSGQGLYRMRIFPIPPRGEKHIRLRYT
                     QLLQSDNGLIEYLYPLNTEKYSAAPLHSVSVKVDITDSEPIGPIYSPSHEVEIRRRDA
                     RHAVIGYEEKSVKPDTDFQLFFGPAGGEAINGRLLTFKDGREEGSFLLMLAPRADIAA
                     GRLLPKDLVFVLDTSGSMAEKSKMTQAKKALHYCLANLAEGDRFEIVRFSTEAEALFN
                     ALRPATEASIRRADEFVEGLRPIGGTALAEALDLALQPLRGQASSGRLCMVVLITDGK
                     PTVGETSEEAILSQVKKAAGDETVRIFSFGVGSDLNTHLLDRLTAETRAASQYVLPEE
                     DIEVKVSSFFDKIALPVLVAPRLSVTGPVRLSKMAPAALPDLFKGEQLLILGRYEGSG
                     RAEITLQGRVNGRTENWIYRLAFPDRAEEHAFIPRLWASRRIGYLLDQIRLHGEEREL
                     REEVVDLARRYGIVTPYTAWLILEDEEQRHVPLARRTLQAGPEDDFREISGRMVQELY
                     QEKSGEAAVGAAQSLDALKNATGGSALAKANRYFQRGQANAATAEAGVVEQALTGQQV
                     RTIANRTFYQNGAQWIDTEAQKQPDRELVRIQFNSEAWFRLLDLEPLAPQWLSAGANL
                     RLVLAGRLYEIYE"
     gene            4421..5737
                     /locus_tag="PKM23_10090"
     CDS             4421..5737
                     /locus_tag="PKM23_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91863.1"
                     /translation="MKGKGFCIVLVVSLLCGLFRPVPAADTRTLAREAAAAIRAVENM
                     QDNAAAAAKLKEIRAKLDQIRAADPNFAELRILESKYKRLTGLFGAPPAVPSAPAVKT
                     GASAASSSSTGGGASSGTVSAAGKAEALKGWEAIVALKEDFLPRLEEVIPVHVKNIIY
                     DGEHADEALARIAVLQQEAPAVKARVVAFGAKYGRTVDEIDSKIYGLTPKDLSLSLYD
                     PQNQRPDESPGRAWEKLSNGLTNLEEAPRLEAKRILTRVLQNLDLIESFIMDTERDKR
                     YAAVEEKLQMAARFSPQDAEVREWQGKIKAMRIKSKADIEKALDGARFPAAFAGFAGP
                     GDAAGLAASALRYFTQEGGWEPNETVVKVVVAGNWVVAATNIFGEPIQWGLPIWAAAY
                     RNDSKEIARVFRLTILTQEGLGVAKRPPWTGVWTGDSFRMRTANVR"
     gene            5799..>6270
                     /locus_tag="PKM23_10095"
     CDS             5799..>6270
                     /locus_tag="PKM23_10095"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HNY91864.1"
                     /translation="MQKIKKSLQPIRLIDQSTPVLLYRSCMVLLTNLIPLYGVWRLGW
                     SSFILILLFIIEGAIVLIMDLVKRRWVTTKEKGKVLFFEFVFITFFGFFAILIFGRDE
                     AAMDLIETTRSSFKAARALPLWPVLGILLGRLLRTGEELMQAGVLHGGEGKPIYF"
BASE COUNT         1129 a         1803 c         2088 g         1250 t
ORIGIN      
        1 gcagcgcctg gccgccgcgc agaggtaatc ccactgctgt tcgaaatgaa cacggcgccc
       61 ggaatttaat ccgggcgccg ttttgcttgc tctttccggt atgaggtgaa cacgcatgtt
      121 gttcgaccat ttcaatctga tcgcccgatt ttacgaccgg gcgatcggcc cgcacgaagc
      181 ggaaaagctc ggcgcgcagc tggccctgcc cgaaaacggc cggctcctgg atgtggccgg
      241 cggcaccggg cgggtgctga gcggactggc gtcctggcgg gggagcatgg ttctgtgtga
      301 ctatgcgatg ggcatgctgc ggcaggcgca ggcgaaaaag gtcgccttgc ccgcccgctc
      361 ggctgcggag cagatgcctt tcgccgacgg ccgcttcgag cgggtgttga tggttgatgc
      421 cctccatcac gtcgccgatc agcctcaaac cctgcgcgag atgtatcgtg tgctttcccc
      481 tggcggccgt atggtcatcg aagagttcga tatccgacac tgggcgatca ggatcgtcgc
      541 tttactcgaa aagatcgccc tgatgcgaag ccattttatg ccgctgtcag agattgccgg
      601 gatcctgcag aaggcgggcg ctcatgtgga gatccgcatg gatggggcgg ccagggcctg
      661 gatcgtggcg gaaaaaccgc ccgcttcata acagccaggc cgtccgctgc ggtacaggag
      721 agttataata acaggggaat gcgcccggga gaatcccggg cgctattatt tgggggagag
      781 ccgcctcaga aggtatagtt ggctttttct gcctgggtgc gcagggcctt gctcatcgcc
      841 ttgtcgaggg tgttgacctc gccgctttgt gaggccttgt tctgttcctc ggcgatgaag
      901 gccgcccgct taacccgcag cccatccatc ttttcctgta tcgcctttct ctgcgcatat
      961 ttctgatcga gatacttttg ccgctcggcc gcggggaggc tgcgcatctc ttcaggcagt
     1021 tcctccttct tcagctccgc cagcctgacc tctttctgct gcacggcatc gacgagatcc
     1081 cagccgctgt tgctgtagat tgccgtcgat ttggtgatcg cccgctgcac cagggaggcg
     1141 ggcgaggcct tggcggcatt caggtcctgg cgctcctggc tcgccttgcc ggcggctccc
     1201 gcctggccat aagctacgta ggtcttgttc agctcggcgc ccaggtcggc caactcctgg
     1261 tcaaaggggg tcggaggatc gatgcttctg gcattctggt cgatgttgaa gtaggagcca
     1321 tcggcgaggt cggcgccctc tttccagctg gtttcgatgc cctcctggtg gtcgccgcaa
     1381 aagatggtgt tgacgaggat gcctttggcg atcgccttct tgcagctggt gcgaaagtcg
     1441 acccgtccct gggtgaaggg ttcattgccg gcgatgaaga tggcacggta gtcgttcgtg
     1501 cccgtgcccc actgcagctc ctccacagcc tgaccgatga cggtgccgca gtactcatcc
     1561 ccgccgtggg tggtgagccc gaagaggagg tcggagacct tgtcgaggtc agcggtgagg
     1621 ggcgtcagct gccggacata accggtgcgc tcgcccaatt caggggatcc atagtggtaa
     1681 agagcaacgg cgatctccgg ccgcctgccg tcgactcggg ccgggatgaa ctcgttgacg
     1741 attttccaga gctggctctt ggcctgatcg atgaggccgt ccatcgagtt gctggtgtcg
     1801 agcagcaggg cgagctgaac gcgggggggc tgctctgaaa tcgccttcct cgggcaaagg
     1861 accagaacag ccagaaggag gaggaagagc agcaaggcga cacgcacaat tttcatggtt
     1921 tctttccttt ctgtactcaa gcgcagattc cgctattcgt aaatttcata gagccggccg
     1981 gccagcacca ggcgcagatt ggcgcccgcc gagagccact gcggcgcgag gggttccaga
     2041 tcgagaagcc tgaaccaggc ctccgagttg aactggatgc gaaccagctc ccggtcgggc
     2101 tgtttttgcg cctcggtgtc gatccattgc gcaccattct gataaaaggt gcggttggcg
     2161 atggtgcgga cctgctgtcc ggtcagtgcc tgttcaacca cgccggcctc cgcggtcgcg
     2221 gcgttagcct ggccgcgctg gaaataacgg ttggctttgg ccagcgcgct gccgccggtg
     2281 gcgtttttaa gggcgtccag ggattgggcc gcgccgacgg cggcttcccc gctcttctcc
     2341 tggtagagct cctgcaccat cctgccggaa atttcgcgga aatcgtcctc cggaccggcc
     2401 tgcagcgtgc gccgggccag gggcacatgg cgctgctcct cgtcctcgag aatcagccag
     2461 gcggtatatg gggtgacgat gccgtagcgc cgggcgaggt cgacgacctc ctcgcgcagc
     2521 tcccgttcct cgccgtggag gcgaatctgg tcgagcagat agccgatgcg gcgcgaggcc
     2581 cagaggcgcg ggatgaaggc gtgctcctca gcccggtcag gaaaagcgag ccggtagatc
     2641 cagttctcgg tgcggccgtt gacgcggccc tgcagggtga tctcggcgcg cccgctgccc
     2701 tcgtagcggc cgaggatgag gagctgctcg cccttgaaga ggtcgggcag cgcagcgggg
     2761 gccattttgc tgaggcggac cggaccggtg acagagaggc gcggcgcgac cagcaccggc
     2821 agggcgatct tgtcgaagaa ggaggagacc ttgacctcga tatcctcttc ggggaggaca
     2881 tactggctgg cggcgcgggt ctccgcggtc agtcggtcga ggaggtgggt gttgaggtcg
     2941 ctgccgaccc cgaagctgaa gatgcgcact gtttcatccc cggccgcttt tttcacctgg
     3001 ctgagaatgg cctcctcgct ggtctcgcct acagtgggtt tgccatcggt gatcagtacg
     3061 accatgcaga ggcgcccgga ggaggcctga ccgcggaggg gctgcagggc cagatcgagg
     3121 gcctcggcca gggcggtgcc gccgatcggc ctcagcccct cgacgaattc atccgcccgg
     3181 cggatgctgg cctccgtggc cggcctcagc gcgttgaaga gggcctccgc ctcggtcgag
     3241 aagcggacga tctcgaagcg gtcgccctcc gcgagattcg ccaggcagta gtgcagggcc
     3301 tttttcgcct gcgtcatttt cgatttttcg gccatcgatc ccgaggtgtc gaggacaaaa
     3361 accagatcct tggggaggag gcgcccggcg gcgatgtcgg cccgcggggc cagcatgagg
     3421 aggaagctcc cctcctcgcg gccgtccttg aaggtgagca ggcggccgtt gatcgcctcg
     3481 ccgccggccg ggccgaagaa gagctggaaa tcggtatccg gcttgaccga cttttcctca
     3541 tagccgatca cagcgtgacg ggcgtcgcgg cggcggatct ccacctcatg gctgggggag
     3601 tagatcgggc cgatgggttc agagtccgtg atgtccactt taaccgaaac gctgtggagc
     3661 ggggcggccg agtatttctc ggtgttgagg ggatagaggt actcgatcag gccgttgtcg
     3721 ctctgcagca gctgggtata gcgcaggcgg atgtgttttt ctccgcgcgg aggaatggga
     3781 aaaatgcgca tccggtagag cccctggccg ctgtattcga gcagggccgg atcgcgccgg
     3841 gcgcggacga tctcctcata gaggcggcgc gccttgccgg catcgagcag ctcggcctcg
     3901 acgctgcggc cgtcgatctc catcgagaac tggtccagct gccctccgcg cgggatcgga
     3961 aagaggtagg tgccctcgag ttcatgcggg tggggattat agaaaatctg atcgatctcc
     4021 gtgaccgcca cctgctcttc gatcttcacc gagacatgat ggtagcgcac ctcgaggggc
     4081 gccagggccg ggggaaaatg gggcggaaag gggggacgcg gaaggcgggg gataatgatc
     4141 atgccgtcgg ccagcagtgc agccgggcct gtgaagacga gcaccaggag aaaggctgtg
     4201 aaaagtcgcc gcatgaccgg ctcctttcga taaaaaagag tttctaccta tccaactaca
     4261 aaaggggaga aaagttcagc cgatttttac gccgggctgt gagaatacaa aaattttctc
     4321 catttcgtca aatttgcttt taggtaacgg atagattcaa tatcttgttt taagagggta
     4381 gtggaggctt ttgcagtgaa tccaaccggt gaggtgtgtg atgaaaggga aaggcttttg
     4441 cattgtgctg gtggtgagtt tgctttgcgg tctgttccgg ccggtgccgg ctgcggatac
     4501 cagaaccctg gcccgggagg ccgctgccgc catccgggcg gtggagaaca tgcaggacaa
     4561 cgcggctgcg gcggcaaagc tgaaggagat cagggctaaa ctcgaccaga tccgcgcggc
     4621 ggaccccaat ttcgccgagc tgcgcatcct ggagagcaaa tacaagcggc tgaccggctt
     4681 gttcggcgcg ccgcctgctg tgccctcagc gcctgcggtc aaaaccggcg cgtccgcagc
     4741 ctcttcatcc tcaaccggtg gcggcgcgag ttcgggcact gtttccgcgg ctggcaaggc
     4801 ggaggcgctc aagggctggg aggcgatcgt ggccctcaag gaggatttcc ttccccgcct
     4861 tgaggaggtc atcccggtgc atgtcaagaa catcatctac gacggcgagc atgccgatga
     4921 ggccctggcg cgtatcgcag tgctgcagca agaggccccg gcggtcaagg ccagagtcgt
     4981 cgcctttggg gccaaatacg gccggacggt ggacgaaatc gacagcaaaa tttatggtct
     5041 gacgcccaag gatctctctc tcagccttta cgatccccaa aaccagcgcc ccgacgagtc
     5101 gcccggccgg gcgtgggaaa aactcagcaa cggcctgacc aatctggagg aggcccccag
     5161 gctggaggcc aagcgcatcc tcacccgggt gctgcagaac ctcgacctga tcgaatcctt
     5221 catcatggat accgagcggg acaagcgcta tgccgccgtc gaggaaaaac tgcagatggc
     5281 tgcgcgcttc agtccccagg atgccgaggt cagggagtgg cagggcaaga tcaaggcgat
     5341 gcggatcaaa tcgaaagcgg acatcgagaa ggccctggat ggggcgcgtt tcccggcggc
     5401 ttttgccgga tttgccggcc cgggggatgc ggccgggctg gcggcctcgg ccctgcgtta
     5461 tttcacccag gagggaggct gggagccgaa cgagaccgtc gtcaaggtgg tggtggcggg
     5521 caattgggtg gtggcggcga ccaatatctt cggcgagccg atccagtggg ggctgccgat
     5581 ctgggcggct gcataccgca atgacagcaa agagatcgca cgggttttcc ggttgaccat
     5641 tctgacgcaa gagggtttgg gtgtggccaa gcgtccgccc tggaccgggg tctggacggg
     5701 tgattctttc cgcatgcgga cggcgaatgt gcgctgacgc agccctgtaa aacggagggc
     5761 tgcagcggcg gaggtccata atttacaggg gaagaatagt gcagaagatc aaaaaaagcc
     5821 tgcaaccgat tcggctcatt gaccaatcca cgccggtgct tctctaccgc tcctgcatgg
     5881 tcctgctgac taacctgatc ccgctctacg gggtctggcg gctgggctgg agcagcttca
     5941 tcctgattct gctcttcatc attgaggggg cgatcgtctt aatcatggat ttggttaaac
     6001 gccgttgggt gaccaccaaa gagaagggca aggttctctt tttcgaattc gtcttcatca
     6061 ccttttttgg atttttcgcc atcctgattt ttggacgcga tgaagcagcc atggatctga
     6121 tcgagaccac acgcagctct ttcaaggcag ccagagccct gccgctctgg ccggtgctcg
     6181 gaattttatt ggggcgcctc ctgcgcaccg gcgaggagct gatgcaggcg ggcgtcttgc
     6241 acggtggtga aggcaaaccg atctatttta
//
LOCUS       DAOPWU010000194         6176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12639_length_6176_cov_4.899526, whole genome shotgun sequence.
ACCESSION   DAOPWU010000194 DAOPWU010000000
VERSION     DAOPWU010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12639_length_6176_cov_4.899526"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKM23_10100"
     CDS             <1..469
                     /locus_tag="PKM23_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY91865.1"
                     /translation="PLVYLVTSIVSVALAIPLGSLSDRIGKEKILIAGFLIYALVYFG
                     FGITRSVSAIIGLFALYGLYSAATDGIQKALVSDIIDKNKQGTGLGLYNALIGFTLLP
                     ASLIAGALYDRVNSSIPFYFGAVMALLAAVLMFFFCRSRNHPSPAGVVERRRS"
     gene            466..1878
                     /locus_tag="PKM23_10105"
     CDS             466..1878
                     /locus_tag="PKM23_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HNY91866.1"
                     /translation="MNTAVADQEKNRVALSSVIAAFFLTGFKLTTGIMTGSMGILAEA
                     AHSALDLIAAVITLFAVRFSGRPADREHTYGHGKIENLSALAETLLLLLTCGWIIYES
                     VRRLLFTEVHVEVSLWAFAVVILSIIIDFGRSRALARTAKKHNSQALEADALHFSTDI
                     WSSGVVLVGLLLVVLSKRTGIAWLTRADAAAALIVAGIVIHVSFQLGKRTVSALLDAV
                     PAELTAEIGQAIMVPGVLRAGRVRARRSGADAFADVTVMVARDTSLEQAHDIAAAVEE
                     EVHNIMPGSDVMVHLDPVESDNESMLQVIRLTAARHGLGVHGIRIYNIHGKEYMEQHI
                     EVSEDYTLEEAHAQTTRFEEALHDLFPRLAQIITHIEPVGDSSARRQPGQADPGQIRR
                     VLLALPEVREAGLDIHDLLVNSVGGEVNIAFHYALASDLPITDAHLLTERIERSLRQM
                     IPNLGRVVIHTEPRSHPERG"
     gene            2066..3373
                     /locus_tag="PKM23_10110"
     CDS             2066..3373
                     /locus_tag="PKM23_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY91867.1"
                     /translation="MQNSTPHTDLPWLVVSDAGGHIFEIPGLEMAGMRLRERRLPEAA
                     ELIPLPFGSALFQLPGRRPVGYDRSRKRFVVLEEHEGQKIFAVGAFIAPAHTQTLLAA
                     YESTPGAEPLPLFAYSAAGWRDERFFTAGVRVDADIRQDPAYFNARLIEQQAALLLKR
                     YPGNRLIGHLVENCVRRYGCPAAQNLALGRWEAPIPTSPSCNARCVGCISKQPEGCVP
                     ETQTRITFVPTPEEIAEYTVPHLDHAPRPVVSFGQGCEGEPLIQGDLLEQAIQLIRSR
                     TASGVINLNTNASRPEVIDRLCQAGLDSLRISLNSARPEPYRAYYRPAGYDWPEVVES
                     MRIARRRGRWISLNYFIFPGFTDDPEEMAALISLVKNHHIDYIQMRNLNIDPEWYGQA
                     LGLEAAAPRAIGILAWMEQVKAAAPWIRFGYFNPPREDWGAGR"
     gene            3370..4824
                     /locus_tag="PKM23_10115"
     CDS             3370..4824
                     /locus_tag="PKM23_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91868.1"
                     /translation="MTTRFRSLKWGLLVLLALLSTSLWLPQHPWIEDALLQKPAAGYS
                     VHLPAGRILFEPFLGPLLFLSRADDPLQLVLWILFWLMAATLLLPIVQARRNGAAPGR
                     ALRTGLTWLPVTFILLAAVLLIFLFSPFPSSTIEHEQKDVVLVNLHSHTYHSHDGLIS
                     PLRLMHWHERNGFDAFFLTEHNNHRASLELVEAQRTGVLPPEPLLIAGEEYSGSNHIL
                     LLGLTRNFNSKDYPDQAAVDSAHSQGGAVLLAHWFSPQRNTRPLAHYIAMGIDGFEIA
                     NQAEGIFHPEKYTTALRRAAQEHDLLLTGNPDYHGYGPACLAWNALHLPGWRRLEREE
                     RTRAIIDLLRQRDQSRLQILLYRDRMPIPAGLTWLSPLITLLDYFRSLRPVQLLAWLA
                     WTGLLLLFRRFAPRIPEQLRIPLQPRELLSIAGAGAAAVTMLFGLWMLVQHSLLGGEN
                     RIFLEYGTWLSIIGILLFIYCITLLKNLKKLNIF"
     gene            4914..5240
                     /locus_tag="PKM23_10120"
     CDS             4914..5240
                     /locus_tag="PKM23_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HNY91869.1"
                     /translation="MEITEISIRLRNESKLKAFVNVTFDNQFAVKGLKIIQSKKGVLL
                     CMPSRKSEDGMQRDIAHPITKEFRTKLEQEILAEYARVEKRAERGEIIFDNSKNEEWA
                     YVDDEI"
     gene            5511..>6176
                     /locus_tag="PKM23_10125"
     CDS             5511..>6176
                     /locus_tag="PKM23_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91870.1"
                     /translation="MPVTKFLRITGLLAIAGLVIIVPARADIERTVTRSYQISPGGWL
                     RIDADLGTIQVNSGASDELEITVLEIMNTSSEKEADRILKDLKLGFIQEGGELRVTAR
                     YNRSGLLDWGGRRLKLRFNATVPARFNLDLHTSGGSISVGDLEGEVLAKTSGGSLSFG
                     RIIGPVKAKTSGGSIKLAGGRGDADLITSGGSISIGSVEGAVAARTSGGGISLGSATG
                     RAQL"
BASE COUNT         1145 a         2180 c         1722 g         1129 t
ORIGIN      
        1 cccgctcgtc tacctggtca ccagcatcgt ctcggtcgcg ctcgccattc cgctcggatc
       61 gctctccgac cggatcggca aggagaaaat cctcatcgcg ggatttctca tctacgccct
      121 ggtctatttc ggcttcggga tcacccggag tgtaagcgcc atcatcggcc tcttcgccct
      181 gtacgggctc tattcagcgg cgaccgacgg catccagaag gccctggtct ccgacatcat
      241 cgacaaaaac aagcagggca cgggactggg tctctacaat gccctgatcg gcttcaccct
      301 gctgcccgcc agcctcatcg ccggcgccct ttacgaccgg gtcaattcga gcatcccttt
      361 ttatttcggc gcggtcatgg cgcttctcgc cgcggtgctg atgttcttct tctgccggag
      421 ccggaaccac ccctcgcccg ccggagtggt cgaaaggagg aggtcatgaa cactgccgtg
      481 gctgaccagg aaaagaaccg ggtggctctc tcctcggtca tcgccgcctt ctttctgacc
      541 ggattcaaac tcaccaccgg catcatgacc ggaagcatgg gcatcctggc cgaggctgct
      601 cattccgccc tcgatctgat cgcggcggtg atcaccctgt ttgccgtgcg cttttcgggc
      661 cgcccggccg accgcgagca cacctacggc cacggcaaga tcgaaaacct cagcgccctg
      721 gccgagaccc tgctgctgct cctcacctgc ggctggatca tctatgaatc agtcagacgc
      781 ctgctcttca cggaggtgca tgtcgaggtc agcctctggg cctttgcggt ggtcatcctc
      841 tcgatcatca tcgatttcgg ccgctcgcgc gcccttgcgc gtacagccaa aaaacacaac
      901 agccaggcgc tcgaggctga tgcgctccac ttttcgaccg atatctggtc ctcgggagtt
      961 gtgctcgttg ggctgctcct ggtggtgctc tcgaagcgaa ccggcatcgc ctggctgacc
     1021 agggccgatg ccgcagccgc cctcatcgtc gccggcatcg tgatccatgt cagctttcag
     1081 ctcggcaaga ggacggtcag cgccctgctc gatgccgtcc ctgcggagct tactgcggag
     1141 atcggtcagg cgatcatggt cccgggggtg ctgagggcgg ggcgcgtgcg cgcccggcgc
     1201 agcggagcgg acgcctttgc cgatgtcacc gtgatggtcg cacgggacac ctcgctggag
     1261 caggcccacg atatcgcagc agcggtggag gaagaggtgc acaatatcat gcccggttcc
     1321 gacgtcatgg tccacctcga tccggtggag agcgacaatg agtcgatgct tcaggtcatc
     1381 cgccttaccg ccgcacgcca cggcctgggc gtgcacggca ttcgcattta caatatccac
     1441 ggcaaggagt acatggagca gcacatcgag gtgagtgagg attacaccct cgaggaggcg
     1501 cacgcccaaa cgacccgctt cgaagaggcc ctccatgacc tcttcccgag actcgcacag
     1561 atcatcaccc acattgaacc ggttggagat tcgagcgccc gcaggcagcc gggacaggcc
     1621 gatcccgggc agatccgccg cgtcctcctc gcactgccag aggtcaggga ggccggcctc
     1681 gacatccacg acctgctggt gaacagcgtc ggcggcgagg tcaacatcgc ctttcactac
     1741 gccctcgcct ccgatctccc catcaccgac gcccacctcc tcaccgaaag gatcgagcgt
     1801 tccctccggc agatgatccc gaacctgggc agggtggtga tccacaccga gccgcgatcg
     1861 cacccggaaa ggggctgacg gggaagaggc gcccgcagcg gcgaaatccg ccgcaaattg
     1921 agcacaattg cctttgataa taataaatta ttcactaagt ttcttcagcc cggggtgaag
     1981 agatggatca ggcttcaaac agcgggtggc gctcctgaga gcgcggtgga tggcgggcgg
     2041 tgaatcttct ttcctgatgc gaattatgca gaattccaca ccccatacgg atctgccctg
     2101 gctggtggtc agcgatgccg gcggccatat cttcgagatc cccggcctgg agatggccgg
     2161 catgcgcctg cgcgaacgcc gccttcccga ggcggcggag ctcattcccc ttcccttcgg
     2221 cagcgccctc ttccagctgc cggggcgccg gcccgtgggc tatgaccgca gccgcaagcg
     2281 tttcgtcgtg ctcgaggagc atgagggaca aaagatcttc gccgtcggcg ccttcatcgc
     2341 ccccgctcat acccagaccc tgctcgctgc ctatgagagc acgcccggcg ccgagcccct
     2401 ccccctcttc gcctacagcg ccgcgggctg gcgggatgag cgctttttca ccgccggggt
     2461 gcgggtggac gccgatatcc gccaggaccc cgcctacttc aacgcccgcc tcatcgagca
     2521 gcaggccgcc ctcctgctca aacgctaccc cggcaaccgc ctcatcgggc acctggtaga
     2581 aaactgcgtc cgccgctatg gctgccccgc ggcccagaat ctcgcccttg ggcgctggga
     2641 ggcccccatc cccaccagcc cctcctgcaa tgcccgctgt gtcggctgca tctcgaagca
     2701 gccggagggc tgcgtgccgg agacccagac gcgcatcacc ttcgtgccca cccccgagga
     2761 gatcgctgaa tacaccgtac cgcacctcga ccacgccccc cgcccggtcg tcagctttgg
     2821 ccagggctgc gagggggagc ccctcatcca gggcgatctg ctcgagcagg ccatccagct
     2881 catccgctcg cgcacagcca gcggcgtcat caatctcaat accaatgcct cgcggccgga
     2941 agtgatcgac cgtctctgcc aagcaggact cgacagtctg cgcatcagcc tcaacagcgc
     3001 tcgcccggaa ccctatcgcg cctactaccg gccggccggc tacgactggc ccgaggtggt
     3061 ggagagcatg cgcatcgccc gccgccgcgg acgctggatc tcgctcaact actttatctt
     3121 tccgggattc accgatgacc cggaggagat ggccgccctg atcagcctgg tgaaaaacca
     3181 ccacatcgat tacatccaga tgcgcaatct caacatcgat ccggagtggt acggccaggc
     3241 cctcgggctt gaggcggccg cgccccgtgc catcggcatc ctcgcctgga tggaacaggt
     3301 caaggccgcc gccccgtgga tccgcttcgg ttacttcaat cccccccggg aagactgggg
     3361 ggccgggcga tgaccacccg attccgcagc ctgaagtggg gtctgctggt cctgctggcg
     3421 ctcctctcca cctccctgtg gctgccgcag cacccctgga tcgaggatgc cctcctccag
     3481 aagccggccg ccggctactc cgtccatctc ccggccgggc gcatcctctt cgaacccttt
     3541 ctcggccccc tcctctttct cagccgggcc gatgatcccc tccagctcgt gctctggatc
     3601 ctcttctggc tcatggccgc caccctcctg ctcccgatcg ttcaggcccg caggaacggc
     3661 gcggcacccg gccgggctct gcgcacgggt ttgacctggc tgccggtcac ttttatcctc
     3721 ctggctgccg tgctgctcat cttcctcttc tcccccttcc caagcagcac cattgagcat
     3781 gagcagaaag acgtggtcct cgtcaacctg cacagccaca cctatcacag ccacgacggc
     3841 ctcatctcgc cgctgcgcct gatgcactgg cacgagcgca acggctttga cgcctttttc
     3901 ctcaccgagc acaacaacca ccgcgccagc cttgaactgg tggaggcaca gaggacgggt
     3961 gtgctgcccc cggaacccct cctcatcgcc ggcgaggaat actccggcag caaccacatc
     4021 ctcctcctcg gcctgacccg caactttaat agcaaggact atcccgacca ggcggcggtc
     4081 gactccgccc atagccaggg gggcgccgtc cttctcgccc actggttctc cccccagcgc
     4141 aacacccgcc ccctcgccca ctatatcgcc atgggcatag acggctttga aatcgccaac
     4201 caggccgagg gcattttcca tcccgaaaag tacaccaccg ccctgcgccg ggccgcacag
     4261 gaacacgacc tgctcctgac cggcaacccg gactatcacg gctacggccc cgcctgcctg
     4321 gcctggaatg ccctccacct gcccggctgg cgccggctgg agcgcgaaga gcgcacccgg
     4381 gcgatcatcg atctgctgcg ccagcgcgac cagtcgcggc tgcagatcct gctctatcgc
     4441 gaccgcatgc ccatcccggc cggtctcacc tggctttcac ccctcatcac cctgctcgac
     4501 tatttccgct cgctgcgacc ggtgcagctg ctggcctggc tggcctggac agggctgctg
     4561 ctcctctttc gccgcttcgc tccgcgcatc cccgaacagc ttcgcatccc gctccaaccg
     4621 cgcgagctgc tctccatcgc cggggcgggc gccgcggcag ttaccatgct tttcggactc
     4681 tggatgctgg tccagcactc cctgctcggc ggggaaaaca ggatttttct cgagtacggc
     4741 acctggctca gcatcatagg catcctgttg tttatatact gcataaccct cctaaaaaat
     4801 ctgaaaaaat tgaacatttt ttagcgaatc catcccgcaa atatccgcca ctgttggtat
     4861 gaatagaaaa acgaataagg ttcacgggga ttaaagaggg tgtgaggcca tcaatggaga
     4921 tcactgagat cagcatcaga ctaagaaacg agagcaagct caaggccttc gtcaacgtta
     4981 catttgacaa ccagttcgcc gtcaagggtt tgaagatcat ccagagcaaa aaaggtgtgc
     5041 tcctctgcat gccttcgcgc aaatcggaag acggcatgca gcgcgacatc gcccacccca
     5101 tcaccaagga gtttcgcacc aaactggagc aggagatcct ggccgaatac gcccgtgttg
     5161 aaaaacgcgc cgaacgcggg gaaatcatct tcgacaacag caagaacgaa gaatgggcgt
     5221 acgtcgacga cgaaatctga tgcaccggtt ttcacttgct cccggcagct gctcctcatc
     5281 ctcaggattc tgattcctca catatcacgc gcgcagacac gccccggcca ggccgggcct
     5341 ctgcgcgctt tttttgtgcc tccaacagcc tttccgcttc tctttttccc tccccgaacg
     5401 cctcccgatg aaaccgcctg ccggtttctg cgtagtaatg agaagaaaga gcggggagcg
     5461 ccctgctccc gacaagagca cggcaccacc atgccaacaa gggaggcact atgcctgtca
     5521 caaaattcct gcgcatcacc gggctgctgg ccatcgcagg cctggtcatc atcgtcccgg
     5581 ccagggcgga catcgaacgc actgtcacca ggagctatca aatctctccg ggcggctggc
     5641 tccggatcga tgccgatctc ggcaccatcc aggtgaacag cggtgcgagc gacgagctgg
     5701 aaatcaccgt cctcgaaatc atgaacacct cctcggaaaa ggaggcggat cgcatcctca
     5761 aggatctgaa actcggcttt atccaggagg gcggcgaact gcgcgtcacc gcccgatata
     5821 accgcagcgg cttgctcgac tggggcggaa gacggctgaa gctccgcttc aacgctactg
     5881 tccccgcccg cttcaacctc gacctgcaca cctcgggcgg atcaatctcg gtgggggatc
     5941 tcgagggtga ggtgctcgcc aagacctccg gcggtagcct cagcttcggc aggatcatcg
     6001 ggccggtcaa ggccaaaacc tccggcggca gcatcaaatt ggcaggcggc cggggtgacg
     6061 ccgatctgat cacctcgggc ggcagcatct caatcggcag cgtagagggc gccgtcgcag
     6121 cgcgcacatc aggcggcggc atcagcctcg gctccgccac cggccgtgcc cagctc
//
LOCUS       DAOPWU010000195         6154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12696_length_6154_cov_3.975734, whole genome shotgun sequence.
ACCESSION   DAOPWU010000195 DAOPWU010000000
VERSION     DAOPWU010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12696_length_6154_cov_3.975734"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..698
                     /locus_tag="PKM23_10130"
     CDS             408..698
                     /locus_tag="PKM23_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91871.1"
                     /translation="MIYFTSGNHDFYGSSLAAVHEETSALVRDTPNLVWLIGAKPRLL
                     EERVALVGDDGWEDARHGNATGTPVELNDFYLIEELAGLSRSSHERGSCQPT"
     gene            complement(722..1639)
                     /gene="amrB"
                     /locus_tag="PKM23_10135"
     CDS             complement(722..1639)
                     /gene="amrB"
                     /locus_tag="PKM23_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNY91872.1"
                     /translation="MARLDRRGWSRSAGPSWRLAISPHDDFAYTGVLYPELLQNIKAP
                     NLFLLGVAHRAARLGIADSLVFDSFSHWRGPWKDIRISPAREELYSLLSGRCAVISDT
                     LHTLEHSLEALIPFLQYFNREITIVPVLVPAMSPERMEECGKALADAMQQVAEKHNWK
                     WGRDYAIIVSTDAVHYGNEDWGGADMAIFGCDSVGNARALALEKEIIANCLVGKISVG
                     NIRRFSSYTLKPENYKEYQWTWCGRYSVPAALYTAYYLNQAMPLEGELVGYSTSIGAE
                     PIPVNDIGMGRTAIATPCHWVGYAAIGYR"
     gene            2124..3686
                     /locus_tag="PKM23_10140"
     CDS             2124..3686
                     /locus_tag="PKM23_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HNY91873.1"
                     /translation="MTEARMQMLAAMGAYMALVIFIGLYYARRANTSSKDFFIGGRTI
                     GPWITAFSAEASDMSGWLLMGLPGVAYWFGLSEAFWTATGLAIGTWLNWLFVAKRLRT
                     YSALAGDAITIPDFLSNRFREQRKLLMSTAALFILIFFTVYAASCFVTVGKLFATLFG
                     LPYPWMMLLGALFVLTYVVIGGFLAETVSDLIQAVVMIAALGVITLSGIHAAGGLTRV
                     MENIRHIPGFFEFFGIARPLVRDGLQATGAAAEPLFGGAGTYGPLMILSTLSWGLGYF
                     GMPHVLIRFMAIRNPADLDKSRRIAVSWCLLSLGAAVAIGLTGRALYPALFLTRSGAE
                     SIFIALSRSLLPPLLAGMVMSGILAATISSSDSYLLIATSAFAKNIYQRVLRREASDR
                     EVMLVSRASILAITLIGVLIALDEKSIIFNIVSFAWAGFGATFGPVMLFSLFWKRTTR
                     NGALAGMIAGGAMVFIWKLLLRPAGGLFAIYELLPAFLTACIVIVVVSLLSQVAVPEI
                     GEEFDRAQRKEE"
     gene            3695..5491
                     /locus_tag="PKM23_10145"
     CDS             3695..5491
                     /locus_tag="PKM23_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNY91874.1"
                     /translation="MEEHIIVGFAAILFFAVSAQWLAWRLRVPSILVLLGTGLLVGPL
                     LHLIDPDRLLGDLLYPVVSLSVAIILFEGGLSLKVSELRDIGGAVTRLITTAVVITWA
                     LVAWAAYAVIGLSLTTSIIYGAILVVTGPTVIIPLLQQVRPARRVASLARWEGIVNDP
                     IGAILAVLVLEAVLVGGFPQALGTELAAIGKTLIAALLLGVVSAAALILLLKKYLIPD
                     YLDEVITLMVVVVAFTAANTIQKEAGLLVVTIFGILLANQKYVPVRHIITFKENLSLL
                     LISGLFIILAARLQFDDLAQIGWRDGVFVLFLIFIIRPLAIYLSTWRSELNWREKTFL
                     ALLAPRGIVAAAVASIFSFTLVKAGYADAQRLIPLTFFVIICTVTFYGLLAAPLARRL
                     QLAKRNPQGVLFAGAHDWAREMAIALQKEGLEVLIVDTNYANLSAARMQNLTTWHGSI
                     LAENVAEELPLFGVGKLLALTGNDEVNVLATQHFAELFGRAETYQLSCLGKKSGGQNT
                     MAMDQCGRLLFSAEASYGALGADFANGAKIKRTALTEEFGYKEFGELYGQRALPLFLI
                     TEEKKLQVFTADRPLVPRAGQLLICLVKEEAA"
     gene            5588..>6154
                     /locus_tag="PKM23_10150"
     CDS             5588..>6154
                     /locus_tag="PKM23_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91875.1"
                     /translation="MKKFMFFLLASLWLTTSLLARVGDILQTIPSPGLRPTGLAFDGK
                     HLWLADAHNDKIIKVDPRTGAVVASLESPGYHPEGLAWDGRHLWHVDAGEKLLYRIDP
                     ATGRVEQALESNSPNPRGIAWDGRDIWMIDHKSDAVLRVSPVDGMMIQTFPSPAAEPT
                     GITFDGKYLWIADRGTDRIYLVNPADGLC"
BASE COUNT         1161 a         1829 c         1761 g         1403 t
ORIGIN      
        1 ggggatcgct gaaatcccag cggcccaggt actggatgtt gggatcgttg gcgggaattt
       61 cgacggcggc gcgaaggggc aaagccccaa cctgcagcgc gaggacaaag agaaggagac
      121 gatttttcat cagatcattt cccttttgct gagtctcgat acctgggcca ggagtgaatt
      181 gaattaatgg cccatgtgca ttcaatccgg cctggatgca tgcacaatct gatttcaatt
      241 ttagacattg ctgctgtaat tgtcaagctc tttatcgact gctgttaaag agctccggcc
      301 tgttaccccc cgaaatctgg cgcccacctg acatcttgcg ccgcataccc ccgaaatctg
      361 gctctgtcgg gccatttctt cgcaagcttg cctcccggct gcccgggatg atctacttca
      421 cctcggggaa tcatgatttt tacggcagct cattggctgc tgtgcatgaa gagacaagtg
      481 cattggttcg ggacacgccc aatctggttt ggctcatcgg ggcaaaaccc cggcttcttg
      541 aggagagggt ggcactggtg ggggatgatg gctgggagga tgcccgtcat ggtaatgcaa
      601 cgggaactcc ggtagagctc aacgatttct acctgattga ggaacttgcc gggctctccc
      661 gcagcagcca cgagagagga tcctgccaac cgacataggc ttttatccgc aatgcaaggt
      721 attatcggta tccaatggcg gcgtatccaa cccagtggca tggtgtggcg atcgcggtcc
      781 ggcccatgcc gatatcatta accggaatgg gctccgctcc tatgctggtt gaataaccga
      841 ccagttctcc ttccagcggc atggcctggt tcaagtaata agccgtgtag agggcggcag
      901 gtacgctgta tctgccgcac caggtccatt ggtattcctt ataattttca ggtttgagtg
      961 tatagctgct gaaacgccgg atattcccga ctgaaatttt cccgacaaga cagttggcta
     1021 tgatctcctt ttccagtgca agagccctgg catttccgac actatcacat ccaaagattg
     1081 ccatatcggc gccaccccaa tcttcgttcc cgtaatgaac ggcatcggtg gaaacgatga
     1141 tagcataatc ccttccccac ttccagttgt gcttttcagc aacctgctgc atggcatcag
     1201 ccagagcttt cccgcattcc tccatccgct ccggactcat cgccgggacg agaactggaa
     1261 cgatggtgat ttcacggttg aagtattgca gaaagggtat cagcgcttca agcgaatgct
     1321 ccaaggtatg caaagtatcg cttatcacag cgcatcggcc agacagcagg gaatagagct
     1381 cttccctcgc cggcgatatc ctgatatcct tccacggacc tctccagtgg gaaaaagagt
     1441 cgaaaaccag tgagtctgcg attcccagcc tcgccgctct gtgggccaca cccagaagga
     1501 acaggttggg agctttgatg ttctgcaaca gctcagggta gaggaccccg gtataggcaa
     1561 aatcgtcatg agggctgatg gccaatctcc aggaggggcc tgcggatctt gaccaccctc
     1621 tccggtcgag tctggccatc aacgaatcca tctgccatgc atgccgggca aagccgacgg
     1681 tgtccgcgag cggccggatg tagaccgtcg gtctgtttgc gtactgtggg gctctgctgc
     1741 agccggctgc aagtgcaagc agcacggctg aacaaatcac aagaccatat ctcatgcgaa
     1801 aggtacctgt tctttgaaag gtgggtgaaa ggcttgtgct gctatgcggc cgcaagccat
     1861 cttcggcaat ttatacaatc agggagtgaa aagcaaggga ttccccccgt cgggatgata
     1921 agctgcagtc gcccaggcag caaccatgcc cacatgcata gaccatatcc ctgccggcgc
     1981 ggtgagtgga cctcgtcagc ggggaggaaa ggcaaggact ggtttgctgt tgatacttgt
     2041 ttttttgacg caatctatct atctttaatt tgttgttgtg gtcacgcagc aaaacgagtt
     2101 cacttttctt gaagaatgac gctatgacgg aagcgcggat gcaaatgctg gctgctatgg
     2161 gcgcttacat ggccctggtc atcttcatcg gcctctatta cgcccggcgc gccaatacca
     2221 gcagcaagga ttttttcatc ggcggccgga cgatcggacc ctggatcacc gccttcagcg
     2281 ccgaagcctc tgacatgagc ggctggcttt tgatgggcct gcccggagtg gcctactggt
     2341 tcggcctcag cgaagccttt tggacggcga ccggactcgc catcggtacc tggctgaact
     2401 ggcttttcgt ggccaaacgg ctgcgcacct actccgccct cgccggcgac gccatcacta
     2461 ttccagactt tttgagtaac cgtttccgtg aacagcgcaa actgctgatg agcacggcgg
     2521 cgctctttat cctgattttt ttcaccgtct atgcagcgag ttgttttgtc accgtgggca
     2581 agctctttgc aaccctcttc ggcctcccct atccctggat gatgctgctc ggcgctctct
     2641 ttgtcctcac ttacgtggtc atcggaggat tcctcgccga aaccgtctcc gatctcatcc
     2701 aggcggtggt gatgattgcc gccctgggag tgatcaccct gtccggaatt cacgccgcgg
     2761 gcgggctcac ccgggtgatg gagaatatcc gtcacattcc cggctttttc gagtttttcg
     2821 gcatcgcccg gccgttggtc cgcgatggcc tccaggcaac cggcgctgcg gcagagccgc
     2881 tctttggcgg agcgggaacc tatggtccgc tgatgattct ttccaccctc tcctgggggt
     2941 tgggctactt cgggatgcct cacgtcctca tccggtttat ggcgatccgc aatccggccg
     3001 atctggacaa atcacgacgc atcgccgtca gctggtgcct gctctctctc ggcgcagcgg
     3061 tagccatcgg cctgaccggc cgagcgctct atccggctct tttccttacc cggagcggcg
     3121 cggaaagcat ctttattgcg ctgagccgca gcctcctccc gcccctgctc gccggcatgg
     3181 tcatgtccgg catccttgcc gcgaccatca gctcatcgga ctcttatctg ctcatcgcga
     3241 cctcggcttt tgccaagaat atctaccagc gtgttctgag aagagaggct tcagaccggg
     3301 aggtgatgct ggtctccagg gcctccatac tggcgatcac gctcatcggt gttctcatcg
     3361 ccctggatga aaagagtatc atctttaata ttgtctcctt cgcctgggcg ggtttcggcg
     3421 ccaccttcgg cccggtcatg ctcttttccc tattctggaa gcggacgacc cggaacggcg
     3481 ccctggcggg catgatcgcc gggggagcga tggtcttcat ctggaaactc ctgttgcggc
     3541 cggccggcgg actcttcgct atctatgagc tgctgccggc ctttctcact gcctgcatcg
     3601 taatcgttgt tgtttctcta ctctcgcagg tggccgtccc tgagattggt gaggaattcg
     3661 accgggcgca acgcaaagag gagtaagcac agtcatggaa gaacatatca tcgtcggttt
     3721 cgccgccatt ctctttttcg ctgtcagcgc ccagtggctg gcgtggcgcc tcagggtgcc
     3781 ttcaattctg gtcctgctcg gcaccggcct gctggtcgga cccctgctgc atctcatcga
     3841 tcccgatcgt ctgctggggg atttgcttta tccggtcgtc tcactgtcgg tggcgatcat
     3901 cctcttcgag gggggattga gcctcaaggt ttccgagctg cgcgatatcg gcggtgcggt
     3961 gacccgcctg atcaccacgg ctgtggtgat cacctgggcg ctggtcgcct gggcagccta
     4021 cgcagtcatc ggtttgagcc tgaccacctc gatcatctac ggcgccatcc tggtggtgac
     4081 aggtccgacc gtgatcatcc ccctgctgca gcaggtgcgc cccgcgcgca gggtcgcctc
     4141 cctcgcgcgc tgggagggca tcgtcaacga ccccatcggc gccatcctcg ccgtgctagt
     4201 gctggaggcg gtgctggtcg ggggatttcc ccaggcgttg gggaccgagc tggcggccat
     4261 cggcaagacc ctgatcgccg ccctcctgct cggggtggtc agcgcggctg cactgatcct
     4321 gctgctgaaa aagtatctca tccccgatta tctcgatgag gtgatcaccc tgatggtggt
     4381 ggtcgtcgcc ttcacagctg cgaatacgat acagaaggag gccggcctgc tggtggtcac
     4441 tattttcggc atcctgctgg ctaaccagaa atatgtcccg gtcaggcaca tcatcacctt
     4501 caaggagaat ctcagcctgc tgctcatctc cgggctcttt atcatcctgg cggcgcgact
     4561 gcagttcgat gacctggcgc agatcggctg gcgcgatggg gtttttgtcc tattccttat
     4621 tttcatcata cggcccctgg cgatctatct ctcgacctgg aggagtgaac tgaactggcg
     4681 cgaaaagacc tttctcgcct tactggcgcc gcgcggcatt gtcgccgccg cagtggcctc
     4741 gatattctct ttcaccctgg tcaaggcggg ctatgccgat gcgcagcggc tcattccttt
     4801 gacttttttc gtgatcatct gcacggtcac cttttacgga ctgctggcgg cgccgctggc
     4861 gcgccggctg cagctggcga aacgcaatcc ccagggcgtg ctcttcgccg gtgcccacga
     4921 ctgggcgcgg gagatggcga tcgccctgca aaaggagggg ctggaggtac tgatagtcga
     4981 taccaactat gccaatctat cagcggcgcg gatgcagaac ctgaccacct ggcacggcag
     5041 cattctggcg gagaacgtgg ctgaagagct gcccctcttc ggggtgggca agctgctggc
     5101 cctgaccggc aatgacgagg tcaacgttct ggcgacgcag catttcgccg agttgttcgg
     5161 ccgggcggag acctaccagc tctcctgtct cggcaaaaag agcggcggcc agaacaccat
     5221 ggccatggac cagtgcgggc ggctgctctt ctcggcagag gcaagctacg gggccctcgg
     5281 cgctgatttc gccaacgggg cgaagatcaa gcgcacggcg ctgaccgagg aattcggcta
     5341 taaggaattt ggtgagttgt acggacagag agcgctgcct ctttttctga tcactgagga
     5401 gaaaaagtta caggttttca cggccgaccg tcctctggtt cccagggcag ggcagctgct
     5461 catctgtctg gtgaaggagg aagctgcatg aggggggagg actaaaactg gcccctctca
     5521 tgctgaggag ttggcgctta cttttttacg gcggggcaaa taccctaaat cagaacgagg
     5581 agcaacaatg aaaaagttca tgttcttcct cctggcgtcg ctctggctga cgaccagcct
     5641 tctcgcccgc gtgggcgaca tcctccagac catcccctct ccggggctgc ggccgaccgg
     5701 actcgccttt gacggcaaac atctctggtt ggctgatgcc cacaacgaca aaatcatcaa
     5761 ggtcgatccc cgcactggcg cagtagtggc cagcctggag tcccccggct atcatcccga
     5821 gggtctggcc tgggacggcc gccacctctg gcatgttgac gccggcgaaa aacttctcta
     5881 ccgtattgat ccggccaccg gccgggtgga gcaggctctg gaatcgaaca gtcccaatcc
     5941 ccgcggcatc gcctgggacg gccgggacat ctggatgatc gatcacaaga gcgatgctgt
     6001 gctcagggtc tcgccggtcg acggcatgat gatccagacc tttccatcgc ccgccgctga
     6061 gccaaccggc atcacctttg acggcaaata cctctggatt gccgatcgcg gcaccgaccg
     6121 aatctatctg gtcaatccag ccgacggcct ctgc
//
LOCUS       DAOPWU010000196         6149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12715_length_6149_cov_5.361503, whole genome shotgun sequence.
ACCESSION   DAOPWU010000196 DAOPWU010000000
VERSION     DAOPWU010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12715_length_6149_cov_5.361503"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1640)
                     /locus_tag="PKM23_10155"
     CDS             complement(180..1640)
                     /locus_tag="PKM23_10155"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HNY91876.1"
                     /translation="MIQYSKYDSPMSPQSFMGVLQKRGRVVLFLFLAIVGVITIAAFL
                     VPPTYRANARLMINYQLDIEKEHLLSLIQIHDKSYYERLSSEPIIMRMRSILEPVVAA
                     LGLDRRKGSAAEPTPSDRDRAIELLGTDLEVEREKDSNVLRVSYESRNPALAASIVSQ
                     VVEQYIKQRPALERDERESEYFDKQIQQVSAQIDELEKKGMEYKARERVLSVDHQSSI
                     LFETLADYDKRLTEVRTERLSKEARLKVIREQLTGGGEIVIPNTESGNAGGRYLYVNE
                     LSKSLLGLEIRKSSLEQKYTSNHPELASVLAQIDENKKKIDQAVKELIQGEEIDIKAK
                     QAEERALAGSMAQVAYNVTDLSRKEYDLGRLTIGIDDLKSVYSMLLRQREQARLAANR
                     QEYLVQVKVLEAAAVPQRPVSPNRPLWIALGLLLGLVVALGVAFFMEYFDHSVNSADD
                     VHNCLGLPILATIGDFKTPMGKQNPSRPGDEAGAEE"
     gene            complement(1672..2574)
                     /locus_tag="PKM23_10160"
     CDS             complement(1672..2574)
                     /locus_tag="PKM23_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNY91877.1"
                     /translation="MKDKWFRRRWAAAGVAMLFTVMISGCSVRPHSTAEIDGSNKVIV
                     ASAPAREAEAGKEKVQKRTKVVRKSTKAKEQQQVQPEKSAPSPQIVEAKAEPARAYVP
                     GMVYRLGFGDVIDIKFLASPEYNETVSIRPDGRISLQGVGELDALGLSPAELDSVVTA
                     AYAQLLVHPDVTVIVREFGGQKCYVAGEVDHPGSMDVAKGMTLLRAIASAGGPKKSAK
                     MGSVILIRMDGEQRAEATRIDLSYTSMKGGIENDLPVMGNDIIYVPRTFIADVNAFVS
                     QVYDVVLPPFDSWTRYFYWYRGLR"
     gene            complement(2612..>6149)
                     /locus_tag="PKM23_10165"
     CDS             complement(2612..>6149)
                     /locus_tag="PKM23_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HNY91878.1"
                     /translation="LAAALPAAEQSVTKILEIDDPLTNARTTGTQRGGKLSSEGWTTQ
                     TALDYIQYSIPTCAAGEISFNVRGIYASREVFPNLDHDRYGAEVPNSENVHYNLFSMW
                     DEDVDNSWYGVQQWHNPYKCYIHIYGYTAGDLYKWRRMKLRLNVAAFNGGYDDDPHAF
                     EDPAVGPFEWEKSHTYRHRLVWGGGKMQWYMDDVLLKTWDYSTFGAEYAPPDHRIRLG
                     SGLLSRSGGLQVPIGIVYSNFKLYRYTDATPPEVVRLDPQSSGEGAAVDADILVHFSE
                     PMNQSSAQAAFSISPAVAGSVSWVGNALYFQPNVLLQPNTTYTVQVAASAKDNAGNAL
                     SSAFSGSFTTRGLNPATVGRFESIDITLVASGLASTTNRYRDVTLKGVFTGPTRTLEI
                     NGFWDLGDVFKVRIAPTEVGTWTYRITSPVASLNATGSFQCVESGSHGFVRLNPARPY
                     TFMHDDGTPWLWRGDTSWRGFTSLFPYEGRWKEYIDLRAEQGYTAMHSIVVSYINGLG
                     FWKNEGGTCFEEGVESKNYDRLNPGYFQWIDKRIDYANSKGIVPVMFFTWAQEYVEFS
                     EAQFDKFVRYLVSRYAAKNVVWVITGEYEEIIADYGRPISEYEHWGQLIYQLDPYDHV
                     ITLHPSGRGSSAPFAPLAWHGAVMQQTPYNVRDLRRDRIYNKPVVNGEPRYFYPEEED
                     EGPNGPSRVAMWEIISNGGYYTTGFFTTYAPDKGGYDPDALPEEQKWAQYCNTFMGEI
                     PWSEMDPHHELISSGNLMAIPGRNYFAYHSTGGAVTLNLSGHLGSLPAKWLNPVTGQY
                     SAPFVVQFGGTVTLTPPFSGDWVLYIGEATNRDTVPPQVPRELGIESTTHQSLTLVWQ
                     PPVPASDGDQAARYRILRDGVELAVQSETRFSDANLKEGTEYFYEIYAIDDADNSSAG
                     AANGRFKSLADVEPPYLSRVTIAAPDTIIAWFSEPVQAASAESPQNYQILQGLPVLSA
                     RLAADRESVKLATGRHEANRVYTLIARNILDRAAIPNRMGANNVRGYHPETSYTVDLT
                     APAGYQWSWLHTGDTYYVDRTFTLLDIPAVLQNLLWLKSADSDRQISSDRLVSFTLHQ
                     PSTVYVAFDAGAASLPAWLSGWRETGLAISTSDLAALHVYEKEFPAGAVELGGNGGTS
                     SGRMYVALVKPEPEHVIGRRPAVPGRIQTMSN"
BASE COUNT         1158 a         1800 c         1905 g         1286 t
ORIGIN      
        1 caccatggac aaataggtcg cgctgcatcc gagcaggaat gccaactcat attgcttgag
       61 ttccgcttcc cagcgtttga ttttcaacat atttttcatt tccggatctc cattatcttg
      121 gtcaacagct tccgttgctt cgccatccag cgttcatctt gctgttttga caccgctgtc
      181 tactcttcgg cgcccgcttc atcaccggga cgcgatggat tttgcttgcc catcggggtc
      241 ttgaaatcac cgatcgtggc gagaatcggc agccccaggc aattatggac atcatcggca
      301 ctgttgaccg agtggtcgaa atactccatg aaaaaggcca cacccagggc gaccacgagc
      361 ccgagcagca ggccgagggc gatccagagg ggacggttgg ggctgaccgg acgctgcggc
      421 accgcggccg cttcaagcac cttcacctgc accaggtact cctggcggtt ggcggccagt
      481 ctggcctgct cgcgctgacg gagcagcatc gaatagaccg acttgagatc atcgatgccg
      541 atagtcagcc ggccgagatc gtactccttg cgcgagagat cggtgacgtt gtaggcgacc
      601 tgcgccatgc tgccggccag ggcccgctcc tccgcctgtt tggccttgat gtcgatctcc
      661 tcgccctgga tcagttcctt gaccgcctga tcgatctttt tcttgttctc atcgatctgg
      721 gccaggaccg aagccagctc gggatggttg gaggtgtatt tctgctcaag actgctcttc
      781 ctgatctcga ggccgagcag gctcttgctc aattcgttga cgtagaggta gcgtccgccc
      841 gcgttgccgc tctcggtgtt gggaatgacg atctcgccgc cgccggtgag ctgttcgcgg
      901 atcaccttga gtctggcctc tttggagaga cgctcggtgc gcacctcggt cagccgcttg
      961 tcgtaatcgg cgagggtttc gaacaggatc gaggactgat ggtcgacgga gagcaccctc
     1021 tcgcgcgcct tgtactccat ccccttcttc tccagctcgt cgatctgggc gctgacctgc
     1081 tggatctgct tgtcaaagta ttcactttcg cgctcatcac gctccagcgc aggccgctgc
     1141 ttgatatatt gttcgaccac ctggctgacg atgctggcag cgagggcggg attgcggctt
     1201 tcatagctca cccgcagaac gttggagtcc ttttcacgct cgacctcgag gtcggtgccc
     1261 agcagttcaa tggcgcgatc gcggtcagag ggggtgggct ctgcggccga acccttcctg
     1321 cggtccagcc ccagtgccgc caccaccggc tcgagaatgc tgcgcatgcg catgatgatg
     1381 ggctcggagc tgaggcgctc gtagtaggat ttgtcatgaa tctggatgag gctcagcaga
     1441 tgctcttttt caatgtcgag ctgataattg atcatcagcc gggcgttggc gcgataggtc
     1501 ggcggcacca gaaacgccgc gatggtgatc accccgacga tggcgagaaa gaggaacaga
     1561 acgactcgcc ctcgtttttg cagtacgccc atgaaacttt gcggcgacat ggggctgtca
     1621 tacttgctgt actgaatcat ggtactctcc tcgtgcttgc tgaatgatgg ctcaccgcag
     1681 tccgcggtac cagtagaaat agcgcgtcca ggaatcgaac ggcggcagaa caacgtcata
     1741 gacctgtgag acaaaggcat tgacatcggc aataaaagtg cgcggcacgt agataatgtc
     1801 gttgcccatc accggcaggt cgttttcaat gccacccttc atcgaggtgt aggagagatc
     1861 gatacgtgtg gcttcggcgc gctgttcgcc atccatgcgg atgaggatga cactgcccat
     1921 cttggcgctc tttttcgggc cgccggcaga tgcgatcgca cgcagcagag tcatgccctt
     1981 ggcgacatcc atgctccccg ggtggtccac ctcgccggcg acataacatt tctggccgcc
     2041 gaattcccgg acgatgacgg tgacatcggg gtggacgaga agctgggcat aggccgcggt
     2101 caccaccgaa tcgagttcgg ccggcgacaa cccgagggca tcgagctcgc cgacgccctg
     2161 cagggagatg cggccatccg gacggatcga gaccgtctcg ttgtattcgg ggctggccag
     2221 aaacttgatg tcgatgacgt cgccaaaacc gaggcgatag accataccgg gcacgtaggc
     2281 ccgggccggc tcagctttgg cctcgacgat ctgcggactc ggggcacttt tttcaggctg
     2341 aacctgctgc tgttctttcg ctttggtgct cttcctgacc accttggtgc gcttctgtac
     2401 cttctccttc ccggcctcag cctccctggc cggggctgat gcgacaatca ccttgttgct
     2461 gccgtcgatc tccgcggttg aatgcggacg cacgctgcag cctgatatca tcaccgtgaa
     2521 gagcatggcc acaccagcgg cggcccatct gcggcgaaac catttatctt tcatttgatc
     2581 ctcacacaat tagaaattga actgctgctt gtcagttgct cattgtctga atgcggccgg
     2641 ggacggcagg gcgcctgcca atgacatgct cgggttccgg cttgaccaga gccacataca
     2701 tgcgtcccga gcttgtaccg ccattgccgc ccagttccac ggcgccggcg gggaactcct
     2761 tctcatagac atgcagggca gcgagatcac tggtgctgat cgccaggccg gtctccctcc
     2821 agcccgaaag ccaggcggga agcgatgcgg ccccggcatc gaacgccaca tagacggtcg
     2881 agggctggtg cagggtgaag gagaccaggc ggtcgctgct gatctggcgg tcgctgtcgg
     2941 cacttttaag ccagagcagg ttctgcagga ccgccggtat atcgagcagg gtgaaagtgc
     3001 gatcgacgta ataggtgtcg ccggtatgca gccagctcca ttgatacccc gccggagcgg
     3061 tcagatcgac cgtatagctg gtctccggat gatacccgcg cacgttattg gcgcccatcc
     3121 ggttgggaat cgccgcccgg tcgagaatat tgcgggcgat cagggtgtag acccggttgg
     3181 cctcatgccg gccggtggcc agtttgaccg actcccggtc ggccgcgagg cgggcggaga
     3241 gaaccggcaa gccctgcagg atctggtagt tctgcggcga ttccgcagaa gccgcctgca
     3301 ccggctcgga gaaccaggca atgatggtgt ccggcgcggc aatcgtgacc cggctcagat
     3361 aggggggctc aacatcggcc aggctcttga aacggccgtt ggcggcccct gcactgctgt
     3421 tatccgcatc atcgatggcg tagatctcgt aaaagtactc ggtcccttcc ttgaggttgg
     3481 catcgctgaa gcgggtttcg ctctgcactg ccagctccac accatcacgc aggatgcggt
     3541 acctggcggc ctgatccccg tccgaggccg gcacgggagg ctgccagacc agggtaaggc
     3601 tctggtgggt tgtactctcg atgcccaact cccgcgggac ctgcggcggt acggtatccc
     3661 gattggtggc ttcgccaatg taaaggaccc agtcgccgct gaagggcggc gtcagggtga
     3721 cggtgccgcc gaactgcacc acaaagggag cactgtactg gccggtgacc ggattgagcc
     3781 atttggccgg caggctgcca aggtgccccg aaaggttgag ggtcaccgct ccgcctgtgc
     3841 tgtgataggc gaaatagttc cggccgggga tggccatcaa attgccggag ctgatgagct
     3901 cgtgatgagg atccatctcc gaccagggga tctcacccat gaaggtattg cagtactggg
     3961 cccacttttg ctcctcgggc agggcatcgg gatcatagcc gcccttgtcc ggggcatagg
     4021 tggtgaaaaa tccggtggtg taatacccgc cgttggagat aatctcccac atggcgaccc
     4081 ggctgggtcc gttgggtccc tcatcctcct cttcgggata aaaataacgc ggttcaccgt
     4141 tgacgaccgg cttgttgtaa atgcggtcac ggcggaggtc gcggacgttg tagggggttt
     4201 gctgcatcac cgcaccgtgc caggccaggg gcgcaaaggg agcactgctg cctcggcccg
     4261 aggggtggag ggtgatgacg tggtcatagg gatcgagctg atagatcagc tgtccccagt
     4321 gctcgtattc cgaaatcgga cggccatagt cggcaatgat ctcttcgtac tcgccggtga
     4381 tcacccagac cacattcttg gcggcatatc gcgagaccag ataacggacg aatttgtcga
     4441 actgggcctc actgaactcg acatactcct gggcccaggt gaaaaacatc accggcacga
     4501 tacccttgct gttggcataa tcgatgcgct tgtcgatcca ctgaaaatag ccggggttga
     4561 ggcggtcgta gtttttggat tcaactccct cctcgaagca ggtgccgccc tcatttttcc
     4621 agaagccgag gccgttgatg tagctcacca cgatggagtg catggcggtg tagccctgct
     4681 ctgcacgcag gtcgatatac tccttccagc gcccctcata aggaaataac gaggtgaagc
     4741 cgcgccagga ggtatccccg cgccagagcc agggcgtgcc gtcgtcatgc ataaaggtgt
     4801 agggccgggc gggattgagc cggacaaagc cgtgcgaacc gctctcgacg cactggaagg
     4861 atccggtggc attgagcgag gcaaccggac tggtgatccg gtaggtccag gttcccacct
     4921 cggtcggggc gatgcggact ttgaaaacat ccccgagatc ccaaaagccg ttgatctcca
     4981 aagtcctggt cggaccggtg aagaccccct tgagggtgac atcgcggtag cggttggtcg
     5041 tggatgcaag cccgctggcg accagggtga tgtcgatcga ttcgaagcgc cccactgtgg
     5101 ccggattgag gccgcgagtg gtgaagctgc cgctgaaggc gctgctcagg gcattgccgg
     5161 cgttgtcctt ggccgaggcg gcaacctgca ccgtgtaggt ggtgttgggc tggagcagaa
     5221 cgttgggctg gaaatagagg gcattgccca cccagctgac gctgccggcc accgccggcg
     5281 agatcgagaa ggcggcctgg gccgagctct gattcatggg ctcggaaaaa tggacgagga
     5341 tgtccgcgtc caccgccgcg ccctcgccgc ttgactgggg atccagccgc accacctcgg
     5401 gcggagtggc atcggtgtag cggtagagct tgaaattgct gtagacaata ccgatcggca
     5461 cctgcagtcc gccgctgcgc gagagcagac ccgagcccag ccggatgcga tggtcgggcg
     5521 gggcatactc tgcgccgaaa gtcgaatagt cccaggtctt gagcagcaca tcatccatat
     5581 accactgcat cttgccccct ccccagacca ggcggtgccg gtaggtgtgg cttttctccc
     5641 actcaaaggg gccgacagcg ggatcctcaa aggcatgggg atcgtcatca tagccgccgt
     5701 tgaaagccgc cacgttgagg cgcagcttca tccggcgcca cttgtagagg tccccggcgg
     5761 tatagccgta gatatggatg taacatttgt agggattgtg ccattgttgg acgccgtacc
     5821 agctgttgtc cacatcctca tcccacatgc tgaaaaggtt gtagtggaca ttttcagaat
     5881 ttgggacctc ggcaccgtac ctgtcgtgat caaggttggg aaatacttcc ctgctggcat
     5941 aaatgccccg gacgttgaat gagatctcgc cggctgcgca ggtcgggata gagtattgaa
     6001 tatagtcgag ggcggtctgg gtcgtccagc cctcgctgct caactttccg ccgcgctggg
     6061 tgccggttgt tctggcattg gtgagagggt catcgatttc caggatcttg gtcaccgact
     6121 gttcggcggc cgggagggcc gccgccagg
//
LOCUS       DAOPWU010000197         6141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12746_length_6141_cov_4.582977, whole genome shotgun sequence.
ACCESSION   DAOPWU010000197 DAOPWU010000000
VERSION     DAOPWU010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12746_length_6141_cov_4.582977"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(396..4025)
                     /locus_tag="PKM23_10170"
     CDS             complement(396..4025)
                     /locus_tag="PKM23_10170"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNY91879.1"
                     /translation="MKYYRLYAATLFLLLLVLLSGFAAAQTVRINEVMSSNNAFVADE
                     EGDFSDWLEIYNPNDTAVDLTNWGLSDKKGTPGKWLFPEVAIGPKSWMLIFADSKDRK
                     QAVAHWETVVRRGQIWRYRVGASAIPAGWIQPGYDDSGWSQGASGIGFGDNDDATTVA
                     TGNISVFSRIAFTLADSAAVTAMILDIDYDDGFVAWLNGIEIARANLGQKGVQPAWNL
                     AANPDHEANIYRGLPPDRFLIANFREALRSGRNLLAIQIHNNSTSSSDLSMIPFLSLG
                     YTAPPADAAGSDPRLVLNKTHMHTNFAISASGETLLLSNGAGAIVDSITVPALPTDLS
                     WARVPDGGNKWILAAVPTPGQANNSKEVAEKAAAAEFSKNGGFYQQPFTFTLSCPTPG
                     ATIRYTRDGSEPDSLSTLCSGPVSIGATTVIRARAFAPGLDPGPVITHTFILGQPPAP
                     LPVISLTTDPRNLWDQEYGIYVLGSSYTNQDPYYGANFWEDWERPVHIEFFEPGGEPG
                     FSQDAGTRIFGGWSRARPQKSMAFFARREYGKEEFNYRLFPELPFENYHSFILRNGGN
                     DWDRTLFADGLIHSRLQGIDLDRQAYRPCTVYLNGEYWGILNLREKVNEHYLAQHHGG
                     DPDEIDQLELDGSVLEGTNDHYIALRDFIASKDLSKAENYAWVQSQMEIENFITYQAV
                     QIYVDNRDWPGNNIKYWRPQGEGGRWRWILFDTEWGFGINAYGSGLPYAYNTLDFATS
                     PTQTPGLHGNPPWSTLLLRRLLMNQEFKRKFINRFADLMNSAYREAPTVAHIDSLQGL
                     LSGDMTRHFDKWRQQVWWSSARLWWGSMDSWYGYIRTLRDFALNRPGYMRAHILQKFN
                     IPAVVSLRLICEPAGAGSVLLNDFLTVTAMPWSGTYFSGVPVQVAAKPAPGYLFAGWG
                     GASTSSDAAIEMTITGTTTLRARFIPAGENDARIVINEINYKSADSHDSEDWVELHNS
                     GPAGADLSGWHLKDDDDAHDFIVPPNTLIESGGYLVVGRDTTKFRKLFPQKSIRLAGN
                     LSFGLSSGGDQVRLFDAGGALVDSVAFGITTPWPSAPAGQGPSLELRSPELDNSLAAS
                     WVAAAGYGTPGAQNSGYNAVNTPPAGRVPEQFGLMQNWPNPFNAETAIAFTLPAPADI
                     RLQIYALNGRLLRTLAAGRIQAGLHRLSWDGCDEAGLPAASGIYLCLLQSGEARQVRR
                     MLLLR"
     gene            4152..6011
                     /locus_tag="PKM23_10175"
     CDS             4152..6011
                     /locus_tag="PKM23_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNY91880.1"
                     /translation="MMQKAVCLLLAGALAWVMPTYSSASELPPVFSASGENSRQAGAE
                     EPLAEELRALEKSFAGRFGFMAKNLRTGEVIAWRAEEKFPTASLIKLPVMVEYFYQAA
                     AGRLDPLQKVILAPAEVRGGSGLLQLFAPGPEVRLIDAVLLMIVVSDNSATNLVIDAL
                     GSTHAEKLAAVNGRMAALGLENTRLLNRLMAWGTKTDSSESIRYGVGVSTPADMLLLL
                     EKLYHGDLADSLSSKKMIEILGAQLYDSAIPRFLPVETDNILVAHKTGGVTGVATDAG
                     LVLSEKADLAIAAFTEQGPDRRDSADNQMKTAIARAARLAWNHFTGDSALERPFLTSV
                     DWNAFPGGEWARIFLSNAPYPHPSRQEGWHYQDKYFPRDPHYIDSSAVIVIPEGFKPR
                     NGAVDLIVHFHGWNNDDLGVLERYQLPQQLAASRRNAVLVLAQGPWRAQDSGGGKMEE
                     EGGCKRMVEEILAVLRAERRIGAEDRLGKVIISAHSGGYRPAIYAVSRGGLQKEISEV
                     YLFDAFYALTEELIPWLKSDIKHRLRSVYTEHLAGENAAFRELLRTHRLRFAEAAPRD
                     ASGQQTEMADERAERTKKLSVLFEPTAECHSCVIFGRFQRWLEASSLGEIEQK"
BASE COUNT         1148 a         1744 c         2121 g         1128 t
ORIGIN      
        1 cgcaggctgg tatgtgagga gtgattattc ataatagtaa ttattactaa tataagcctt
       61 ttcgatctca ctgtcaagta aaatcggtga accggccggc tcgataccgg gtgggcacga
      121 gatggagtgg aggctgtggc ggctccgtgg cgtgagccgg aggccaccca ggccggctcg
      181 ataccgggtg ggcacgagat ggagccggcc ggccggctcc gggtgggtac gagatggagt
      241 ggaggctgtg gcggccgcgc gggcgtgagg cggaggccac ccgggctggc tggataccgg
      301 gtgggcacga gatggagccg gctggccccg ggtgggcacg agatggagcc ggccgggtgg
      361 gcgccagagt ggccggccgg gaatgagggt caagctcagc gcagcagcag catgcgccgt
      421 acctgacgcg cctccccgct ctgcagcagg cagagataga taccgctggc cgccggcagc
      481 ccggcctcat cgcagccgtc ccagctgagc cggtgcagac ccgcctggat cctgccagcc
      541 gccagggtgc gaagaaggcg tccgttcagg gcgtagattt gcaggcggat atcggccggc
      601 gccggcaggg tgaaggcgat agcggtttcg gcgttgaagg gattgggcca gttctgcatg
      661 agcccgaact gctccggcac ccggccggcc gggggagtat ttaccgcgtt atagcctgaa
      721 ttctgtgctc cgggggtgcc gtagccggca gctgccaccc agctggcggc gaggctgttg
      781 tccagctccg ggctgcgcag ctccaggctc gggccctggc cggctggtgc ggagggccag
      841 ggagtggtga taccgaaggc gaccgaatcg accagcgcgc cgccggcgtc gaagagccgg
      901 acctggtcgc cgccgctgct caggccgaag gagaggttgc cggcgaggcg gatgctcttt
      961 tgcggaaaga gtttgcggaa tttggtcgtg tcccgtccca ccaccagata gccaccgctc
     1021 tcgatcaggg tgttgggggg gacgatgaaa tcgtgggcat cgtcatcatc cttgagatgc
     1081 cagcccgaga ggtcagcacc ggcggggccg ctgttgtgca gttccaccca gtcctcgctg
     1141 tcgtggctgt cggcggattt gtaattgatc tcgttgatga cgatgcgggc gtcattttct
     1201 ccggcgggaa tgaagcgggc cctgagggtg gtggtgccgg taatcgtcat ctcgatggcc
     1261 gcatcggatg aagtggatgc cccgccccag ccggcgaaga ggtagcccgg ggctggtttg
     1321 gcggcgacct gcaccggcac acccgaaaag taggtgccgc tccagggcat ggcggttacg
     1381 gtgagaaaat cgttgaggag cacgctcccc gcccctgcgg gttcgcaaat gaggcggagg
     1441 gagacgacag ccgggatgtt gaatttctgc aggatgtggg cgcgcatgta gccaggccgg
     1501 ttgagagcga aatcacgcag ggtgcggata taaccgtacc agctgtccat cgagccccac
     1561 cacagcctgg ccgagctcca ccacacttgc tgccgccatt tgtcgaaatg acgggtcata
     1621 tcgccggaga ggaggccttg cagggaatcg atatgggcga cagtcggggc ctcgcgatag
     1681 gccgagttca tcaggtcggc gaagcggttg atgaatttcc gcttaaactc ctgattcatc
     1741 agcagccggc gcagcagcag ggtggaccag gggggattgc cgtgcaggcc gggagtctgg
     1801 gtggggctgg tggcgaagtc aagggtatta taggcatagg gcaggccgga gccataggca
     1861 ttgatgccga acccccactc ggtgtcgaag aggatccagc gccagcggcc cccttcgccc
     1921 tgcggacgcc agtacttgat gttgttgccc ggccagtcgc ggttgtcgac gtaaatctgc
     1981 accgcctggt atgtgataaa gttctcgatc tccatctggc tttgcaccca ggcgtaattc
     2041 tccgccttgg agaggtcctt gctggcgatg aaatcgcgca gggcgatata gtggtcgttg
     2101 gtgccctcga gcacactgcc gtccagttcg agctgatcga tctcgtcggg gtcgccgccg
     2161 tggtgctggg cgagataatg ttcgttcacc ttctcgcgca ggttgaggat accccaatat
     2221 tcgccgttga gatagacggt gcagggacgg taggcctggc ggtcgaggtc gatgccctgg
     2281 agccgggagt ggatgaggcc gtcggcgaag agggtgcggt cccagtcgtt gccgccgttg
     2341 cgcaggatga aggagtggta attttcaaag ggcagctcgg ggaagaggcg gtagttgaac
     2401 tcctccttgc cgtattcgcg ccgggcaaag aaggccatcg acttttgcgg gcgggcgcgg
     2461 ctccagccgc caaaaatgcg cgtgccggcg tcctggctga aacccggctc tccgcccggc
     2521 tcgaagaatt cgatatggac cggccgctcc cagtcctccc agaaattggc gccgtaatag
     2581 ggatcctgat tggtatagct ggatccgagc acatagatgc catactcctg gtcccagagg
     2641 ttgcggggat cggtggtcag cgaaatcacc ggcaggggag cgggcggctg gccgaggata
     2701 aaggtatgtg tgatgaccgg acccggatcg aggccggggg cgaaggcgcg cgcccggatg
     2761 acagtggtgg cgccgatgct gacgggtccg ctacagagcg tcgaaaggga atcgggctcg
     2821 ctgccgtcgc gcgtgtagcg gatcgtcgca cccggggtcg ggcagctcag ggtgaaggtg
     2881 aagggctgct gataaaagcc gccgtttttg gagaactcgg ccgcggcggc cttttcggcg
     2941 acctccttgc tattgttggc ttgtcccggt gtgggcacag cggcgaggat ccacttgttg
     3001 ccgccatccg gcacccgggc ccaggagagg tccgtgggca gtgcgggcac ggtgatgctg
     3061 tcgacgattg cccccgcgcc attgctgagc aggagggttt ctcccgaagc ggaaatggca
     3121 aagttggtgt gcatatgggt cttgttcagg accagcctgg ggtcgctgcc ggcggcatca
     3181 gcgggcggag cggtatagcc cagggagaga aaggggatca tggacagatc ggaggaggag
     3241 gtcgagttgt tgtggatctg gatggccagg aggttgcgcc cggaacggag tgcctcgcgg
     3301 aaattggcga ttaaaaagcg gtcggggggc aggccgcggt agatgttggc ttcgtggtcg
     3361 ggattggcgg ccaggttcca ggcgggctgc acccccttct ggccgagatt ggcgcgggcg
     3421 atctcgatgc cgttgagcca ggcgacaaaa ccgtcgtcat aatcgatatc gagaatcatc
     3481 gccgtcaccg cggccgagtc ggccagggtg aaggcgatgc ggctgaagac ggagatatta
     3541 ccagtggcga ccgtggtggc gtcgtcattg tcgccgaagc cgatgccgct tgccccctgg
     3601 ctccagccgc tgtcatcata gccgggctgg atccagccgg cggggatggc cgaggcaccg
     3661 acgcggtagc gccagatctg cccgcggcgg accacggtct cccaatgcgc caccgcctgc
     3721 ttgcggtcct tgctgtcggc gaagatgagc atccagctct tcggcccgat cgcgacctcc
     3781 gggaagagcc attttccggg cgtccccttt ttatcgctca gtccccagtt ggtgagatcg
     3841 acggcggtgt cgttcggatt atagatttcg agccagtcgg aaaaatcgcc ctcctcatcg
     3901 gcaacaaagg cgttgttgga ggacatgacc tcgttgatgc ggacggtctg ggcggccgca
     3961 aaaccggaaa gcagcacgag gaggagcaga aagagcgtgg cagcatacaa tcggtaatat
     4021 ttcatgataa ggacatcctt gctgaagagt gagcgttgac cgggtgatcg acaaaaccca
     4081 atttatgctt ttcttttaac ttttaccata ctattttaag ccgaagggat cggttgaaaa
     4141 tgaaagggga catgatgcag aaagccgttt gtttactttt ggccggggcg ctggcctggg
     4201 tcatgccaac gtattccagt gcaagcgaac tacccccggt tttttccgcc agcggagaaa
     4261 actcccggca ggccggagcg gaagagccgc tggcagaaga actgcgcgcc ctggaaaaaa
     4321 gcttcgccgg ccgttttggc tttatggcca aaaacctgcg caccggtgag gtgatcgcct
     4381 ggagggcgga ggaaaaattc ccgacggcca gcctcatcaa gctgccggtg atggtggaat
     4441 atttttatca ggccgcggcc gggcgtctcg accccctgca aaaggtgatc ctggcgcccg
     4501 cagaagtccg gggcggttcg ggcctgctgc agcttttcgc ccccgggccg gaggtgcgcc
     4561 tcatcgacgc cgtgctgctg atgatcgtgg tcagtgacaa cagcgccacc aacctggtca
     4621 tcgatgcgct cgggagcacg catgcggaga aactggccgc cgtcaatggc cgcatggccg
     4681 ccctcggact cgagaacacc cgcctgctca accgcctgat ggcctggggg accaagaccg
     4741 actcctccga gtcgatccgc tacggtgtag gggtctccac cccggccgat atgctcctac
     4801 tgctggaaaa gctttatcac ggtgatctcg ctgactcgct ctcgagcaaa aagatgatcg
     4861 aaattctcgg cgcgcagctt tacgattcgg ccatcccgcg ctttttgccc gtcgagaccg
     4921 acaatatcct ggtggcccac aagaccggcg gcgtcaccgg cgtcgccacc gacgcgggac
     4981 tggtgctctc ggaaaaagct gatctggcca tagccgcctt taccgagcag gggcccgacc
     5041 gccgcgacag tgccgacaat cagatgaaaa cggcgatcgc ccgggcggcg cggctggcct
     5101 ggaatcattt caccggggat tccgccctgg agcgcccctt tttgaccagc gtcgactgga
     5161 acgcctttcc cggcggcgaa tgggcgcgga tctttctgag caacgcaccc tatccccatc
     5221 cctcgcgcca ggagggatgg cactatcagg acaagtactt tccccgcgat ccccactata
     5281 ttgacagcag cgccgtgatc gtcatcccgg agggattcaa gccgcggaac ggggcggtgg
     5341 acctcattgt ccattttcac ggctggaaca acgacgatct cggtgtgctg gagcggtatc
     5401 agctgccgca gcagctggcc gcatcgcgcc gcaacgccgt gctggtgctg gcgcaggggc
     5461 cctggcgcgc ccaggattcg ggcggcggca agatggagga ggagggggga tgcaaacgga
     5521 tggtcgaaga gatccttgca gtgctgcggg cagaacggcg catcggcgct gaggatcggc
     5581 tcgggaaggt gatcatcagc gcccacagcg gcggctaccg cccggcgatt tatgcggtca
     5641 gtcgcggcgg gctgcagaag gagatcagcg aggtttatct cttcgatgcc ttttatgccc
     5701 tcaccgaaga gctcatcccc tggctcaagt cggacataaa gcatcgcctg cgttcggtct
     5761 ataccgaaca tctcgccggc gagaatgcgg cattccggga actgctgcgc acccaccgcc
     5821 tccgcttcgc agaagcggcc ccgcgcgacg cctccgggca gcagacggag atggccgacg
     5881 agcgcgccga gcggacaaaa aagctgagtg tactcttcga gccgaccgcc gaatgccaca
     5941 gctgcgtcat tttcggtcgt tttcagcgct ggctggaagc gagttcgctg ggagaaatcg
     6001 aacagaaata aatggcactg gtccgtgacg ggtgaggtcg aggggttgtg ggctgcggag
     6061 agataaaagg ataagcccca cccggcagcc aggccgggtg gggagactat gtttacttgg
     6121 gcagcgccgc cgccagggtc g
//
LOCUS       DAOPWU010000198         6130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12772_length_6130_cov_5.925267, whole genome shotgun sequence.
ACCESSION   DAOPWU010000198 DAOPWU010000000
VERSION     DAOPWU010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12772_length_6130_cov_5.925267"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PKM23_10180"
     CDS             complement(<1..501)
                     /locus_tag="PKM23_10180"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNY91881.1"
                     /translation="MLKMKRNLGWLLLLLLVPGLALAQTTGKISGKVLDARNGQPLPG
                     ANVIVEGTSSGAVADLNGDFFIINVAPGSYTLRAQMMGYETVRMQEVQVSVNRTSEIK
                     IRMKETVLEGEVVVVQAEKVDMKKDQTSSVRNVSSQQIDALPVENMGAVISMQAGVVN
                     GHFRGGR"
     gene            complement(746..3280)
                     /locus_tag="PKM23_10185"
     CDS             complement(746..3280)
                     /locus_tag="PKM23_10185"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91882.1"
                     /translation="MKKLLLVMVLALMPVLAFGQMINNFDAAPADTNYWNWKSNHGGQ
                     HYEVNTSAAPEKGWVRLTYVTDPKAEGAGSMLYEYSAHNTESWGGYSKIEHFNLDTTA
                     VYDFSKYDSIGFWYNIKDKQTLPGRAHIRFELYEVSDAAEGNATYSAGQCEFYYSFEY
                     VLDNDPGWAWFKLPLLDGRNEDVMDEWNGGAFNRTGWAGVTGNDKFDANKIKGYGFEI
                     SISGAGEGDFSAGSFYLDDLSLIHLAKKPIIFFNGREVANFLGGAWSWGQSAVTIEEG
                     AGVVEKTNAVKWTQGNEWGNGWSGIGWSATAPIDMSSVWESDSLSFMYKAEEGTGAIR
                     FQIEAGAAKVGSFFAPIADNQWHRHALKLSEMAPQDGTTGFDPAAVTVLGIMAEGSAI
                     AGKVVYFTNIWTGTPVIDVVAPNAPAGVAAIPQDGYNLVIWEDVPGEEGEVYNLYASP
                     KPFTAITDPGVLLIASKIAEGTQTFAHYIFSALTDKPKDYYYAVVCKDASSNESPAGI
                     SGPFNNIAKGIPTISLTPPANFVADGDVTEWVNSGIMPFVLKPSISNIAIGEFSSDDD
                     LTVTAWLAIDNDALYVAADVVDDVYSFVEGGNWYEDDGMELYIGLYNQSTVHASFKRG
                     AEPDYSFYTLTTGFMWGNGSGGDFALYPNNTPSNYAFVNFGAADYAIEFKIPLDSLVT
                     HGSVGDTRFQPLNGMRIPIEIMFHDSDVANARAGVLSFSTLNADNAWQSPANWAFTWI
                     GDQEEVTGVAVKDATTVETYALSQNYPNPFNPTTTISYTLPKAGKVAIEVYNMIGQKV
                     ATLVNANQTAGIHTVDFDGANLTSGIYFYRLTAGDFTTMRKMVLMK"
     gene            complement(3463..5286)
                     /locus_tag="PKM23_10190"
     CDS             complement(3463..5286)
                     /locus_tag="PKM23_10190"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNY91883.1"
                     /translation="MGCVRYVFLLVLLIVTNAFPVVYYGGEYRTYEGFVYGRFEASIK
                     PPRGSGTLASFFTYHDFTAATAWNEIDFEILGRYTDDVQVTSIGPGQKWRNSHQVVPF
                     VPQDGFHTYAFEWTPDYIAWFIDGVEYYRQDQTHIQQFVYPQKIMMNLWSSESSGWVG
                     PWDDRILPLFAWYDWVSYASYTPGSGNTGTGNNFTLQWKDEFTSWNQSRWTMGTHTFN
                     GNRVTFRRENVVFRDGCVVLCMTKGPEMGFNDKTPPAVLWARADQDTVTVRFSEAIDP
                     ATVTKANFAITGITIHNAILQPDQQTVKLAVSSLDPTKRNNVVALNIKDTFSPANKLT
                     AQLQQIIMGQPLSLPVRINVGGKAWRDYLPDQEWGPEVEYGYQDGAPRDRSKPIVGID
                     VLNTEEDTLYHTEMHELVFYRVRLPRGSYRVTLKVAEMTFMEPLSRLYNIYVEGRKVA
                     DRLDLAADPGPKAALELTAEAVEVTDGILDIRFEYLWGYDTDWGSLLNGLVIEPAASS
                     VGSNPARRPETMALVTSYPNPFNAETTIAYELPSAGQIRLTVYDRLGRQVEELASGPA
                     AAGPHMVRWTPTLPSGLYFVGLEHEGAAGSHRALNKLVLVR"
     gene            complement(5476..>6130)
                     /locus_tag="PKM23_10195"
     CDS             complement(5476..>6130)
                     /locus_tag="PKM23_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNY91884.1"
                     /translation="QLDLVLALQKSGKPVVVVLLQGRPRIIRPIVDGARAILLAWLPG
                     MEGGRAIADVLFGDANPSGRLPVTYPRSPNDLVLYDHLWTENTGGTNRFNPQWPFGFG
                     LSYTTFAYSDLKLDKSSAARGEVITASVTVTNTGQVAGLETVQLYLSDLVASIAPPNK
                     RLKGFRKIELAPGESKTVYFTLSGEEMSFIGLDGKPVIEPGEFRVTVADLSSNFTLK"
BASE COUNT         1193 a         1694 c         1985 g         1258 t
ORIGIN      
        1 gcggccgccg cggaaatggc cgttgacgac gccggcctgc atcgagatga cggcgcccat
       61 gttttccacc ggcagggcat cgatctgctg cgaggagaca ttgcgcacgg agctggtctg
      121 atcctttttc atgtccactt tttcggcctg gacgactacc acctcgcctt cgaggacggt
      181 ctccttcatg cggatcttga tttcactggt gcggttgacc gagacctgca cctcctgcat
      241 gcgcacggtc tcgtaaccca tcatctgggc gcgcagggtg tagctgcccg gcgcgacgtt
      301 gatgatgaag aaatcgccgt tgagatcggc aaccgcgccg gagctggtgc cctccacgat
      361 gacattcgcg cccgggaggg gctggccgtt cctggcgtcc agaacctttc cggagatctt
      421 gccggtggtc tgcgccaggg caagtcccgg caccaggagc agaagcagaa gccaacctag
      481 gttccgtttc atcttgagca tacgctttcc ttctcttata ctgcgggtta attgaggata
      541 atggcacatc ttcgggatgc cgggaatggc aaggcccgca ggctgcgccg gttacagttc
      601 tgcaaactcc gggttcttct tccgattaga gggaaaaagg gcctgcagtc tgcaggtgaa
      661 ggcgggcgat gcgggcgggc aaagccggta gtgccgaaaa tgaaagcagt cgcggacctt
      721 gcgatccgcg actgctccgg ggaagctatt tcatcagcac catcttgcgc atggtggtga
      781 aatcacccgc cgtcaggcga tagaaataga tgcccgaggt caggttggcg ccatcgaaat
      841 cgacggtatg aatgccggcg gtctgattgg cattgacgag cgtagccacc ttctggccga
      901 tcatgttgta aacctcgatc gccaccttgc ccgccttggg cagggtgtag ctgatcgtgg
      961 tggtcgggtt gaagggattg ggatagttct gcgagagagc gtaggtttca accgtggtcg
     1021 catccttgac cgcgacgccg gtgacttctt cctgatcgcc gatccaggtg aaggcccagt
     1081 tggccgggct ctgccaggcg ttgtcggcat tcagcgtgga gaaagagagc acgccggcgc
     1141 gggcattggc gacatccgaa tcgtggaaca tgatctcgat cgggatgcgc atgccattga
     1201 gcggctggaa gcgggtgtca ccgacagagc cgtgcgtcac cagggaatcg agcggaatct
     1261 tgaattcgat ggcatagtca gcggcgccaa agttgacaaa ggcgtagttg gaaggagtgt
     1321 tgttcggata aagcgcgaaa tcgccgccgc tgccattgcc ccacataaag ccggtggtca
     1381 gggtataaaa gctgtaatcg ggctcggcgc cgcgcttgaa gctggcatga accgtggact
     1441 ggttgtagag accgatgtaa agctccatgc catcgtcctc ataccagttg ccgccttcga
     1501 caaaactgta gacatcatcg accacatccg ccgccacata gagcgcgtca ttatcgatgg
     1561 ccagccaggc ggtcaccgtc agatcgtcat cgctgctgaa ttcaccgatg gcgatgttgg
     1621 agattgaggg tttgagcacg aagggcatga tgccggagtt gacccactcc gtcacatcgc
     1681 cgtcggcgac gaaattggcc gggggagtga gcgagatggt gggaatgccc ttggcgatat
     1741 tgttgaaggg accgctgatg ccggccgggc tctcattgct ggaggcatcc ttgcagacca
     1801 cggcgtagta gtaatccttg ggcttgtcgg tcagggcgga gaagatatag tgcgcaaagg
     1861 tctgggtgcc ttcagcgatc ttggaggcga tcagcagcac gccgggatcg gtgatggccg
     1921 tgaagggctt ggggctggca tagaggttgt agacctcgcc ttcctcaccc ggaacatctt
     1981 cccagatcac caggttgtag ccgtcctgag gaatcgcggc gacgccggcc ggggcattgg
     2041 gggcgacaac gtcgatcacc ggggtgccgg tccagatgtt ggtgaaatag accactttgc
     2101 cggcgatagc ggagccttcg gccatgatgc cgagcacggt gacggcggcg ggatcaaagc
     2161 cggtggtgcc gtcctggggc gccatctcgc tcagcttgag ggcgtgacga tgccactgat
     2221 tgtcagcgat gggggcaaaa aaggagccga ccttggccgc gccggcttcg atctggaagc
     2281 ggattgcccc ggtgccctct tcagccttgt acataaagga aagggagtcg ctctcccaga
     2341 ccgacgacat gtcaatggga gccgtagcgg accaaccgat gccgctccag ccattgcccc
     2401 actcgttgcc ctgggtccat ttcacggcgt tggtcttttc gacgacgccg gcgccttcct
     2461 cgatcgtcac tgcggactgg ccccagctcc aggcgccgcc gaggaaattg gcgacctcgc
     2521 ggccgttgaa aaagatgatc ggtttcttgg ccaggtggat caggctcagg tcatcgagat
     2581 agaaggaacc ggcggaaaaa tcgccttcgc cggcgccgct gatggagatc tcaaagccgt
     2641 agcccttgat cttgttggca tcgaacttgt cgttgccggt gacgccggcc caaccggtgc
     2701 ggttgaaagc cccgccgttc cactcgtcca tcacatcctc attgcggcca tcgagcagcg
     2761 gcagcttgaa ccaggcccaa cccggatcgt tgtcgagaac gtactcaaag ctgtagtaga
     2821 actcgcactg gccggcgctg taggtggcat tgccttccgc agcatcgctg acttcataga
     2881 gctcgaagcg gatgtgagcg cggccgggca gggtctgctt gtccttgatg ttgtaccaga
     2941 agccgatgga atcgtacttg gaaaaatcat agactgcagt ggtatccaga ttgaaatgct
     3001 cgatcttgga atagccaccc cagctctcgg tgttgtgggc gctgtattcg tagagcatcg
     3061 acccggcgcc ttcggctttg ggatcggtga cgtaggtgag gcggacccag cccttttcag
     3121 gggcggcgct ggtgttgacc tcatagtgct ggccgccgtg gttggatttc cagttccagt
     3181 agttggtgtc cgcgggagcg gcgtcaaaat tgttgatcat ctgtccgaat gccagcaccg
     3241 gcatcagggc gaggaccatg accaatagca gtttcttcat tgcttcttcc tccaaagtgg
     3301 tttggttggt ggttttggcc tgcgttggaa agggcggcgc agagtacggg agcacaaggc
     3361 ttcctttctg cgcgggaatc aggcgctttg gatttgagct gccaggcacc tcctttcaca
     3421 tgaatattag atgcgttctg gtaattttgg gtgcacagaa aatcagcgaa caagtacgag
     3481 tttgttcagg gcgcggtgcg agcccgcggc gccctcatgt tcaaggccaa cgaaatagag
     3541 cccgctcggc agggtgggcg tccatctcac catgtgcggg ccggccgcag ccgggccgga
     3601 ggcgagctcc tcgacctgcc tgccaaggcg gtcgtacacc gtcaggcgga tctggcctgc
     3661 actgggaagt tcgtaagcga tcgtggtctc ggcgttaaag ggattggggt agctggtcac
     3721 cagggccatc gtctccggcc ggcgcgcagg attgcttccg accgacgaag ccgccggctc
     3781 gatgaccagg ccgttgagca gggagcccca gtcggtatca tacccccaga gatactcgaa
     3841 gcggatatca aggatgccgt cggtcacctc gaccgcctcg gcagtaagtt ccagagcggc
     3901 tttcgggcca ggatcggccg ccagatcgag gcggtcggcc accttgcggc cctccacata
     3961 gatgttgtag agccggctga ggggttccat gaaggtcatc tccgccacct tgagggtgac
     4021 gcgatagctg ccccggggca ggcgcaccct gtagaaaacc agctcgtgca tctcggtatg
     4081 gtaaagggtg tcctcttcgg tgttgagaac gtcgatgccg acgatgggct tgctgcggtc
     4141 gcgcggggcg ccgtcctggt agccgtactc gacctccggc ccccactcct ggtccggcag
     4201 atagtcgcgc caggccttgc cgccgacatt gatcctgacg ggcagagaga ggggctggcc
     4261 catgatgatc tgctgcagct gcgcggtgag tttgttggcc ggggagaagg tgtccttgat
     4321 gttcaacgcc acgacgttgt tgcgtttggt gggatcgagg gacgagaccg ccagcttgac
     4381 cgtctgctgg tcgggctgaa ggatggcgtt atgaatagtg ataccggtga tggcaaagtt
     4441 ggctttggtg acagtggcgg gatcgatggc ttcgctgaag cggacggtga cggtatcctg
     4501 gtcggcgcgg gcccagagca cggcgggagg ggttttatcg ttgaaaccca tctccggccc
     4561 cttggtcatg caaagcacaa cgcagccgtc gcggaaaacc acgttctccc gccggaaggt
     4621 cacccggttg ccgttgaagg tgtgggtgcc catggtccag cgcgactggt tccagctggt
     4681 gaactcgtcc ttccactgca gggtaaaatt attgccggtg ccggtattgc cgctgcccgg
     4741 cgtatagctg gcgtagctga cccagtcata ccaggcgaag aggggcagga tgcggtcatc
     4801 ccaggggccc acccagcccg atgattcgga actccagaga ttcatcatga tcttctgggg
     4861 atagacgaac tgctggatgt gggtttggtc ctgccggtag tattcaaccc cgtcgatgaa
     4921 ccaggcgata tagtccgggg tccattcaaa agcgtaggta tgaaatccgt cctgggggac
     4981 gaaggggacg acctgatggc tgttgcgcca tttctggccc ggcccgatcg aggtcacctg
     5041 gacgtcgtcg gtgtagcggc cgaggatctc gaaatcgatc tcgttccagg cggtggcagc
     5101 ggtaaagtcg tgataggtga aaaaggaggc gagggtgccg ctgccgcggg ggggcttgat
     5161 ggaggcctcg aagcgtccgt agacgaatcc ttcgtaggtg cggtattccc caccgtaata
     5221 gacaaccggg aaggcgttgg taacgatcag taaaacaagc agaaaaacgt agcgaacgca
     5281 gcccatgcat tctccttctg ttcccggcac tccaaggccg agagaggggt atgatctggc
     5341 tccagggcgg ttacctcaac gcttaaaata gcacaatttt tattctttgt aaaggaaaaa
     5401 aataggtgga tagcgcagga tgcattttac ggttcgggcg cacaaccgga gcggtcatgc
     5461 gcccggaccc aggggctatt taagggtgaa attgctgctc aaatcggcga ccgtgacccg
     5521 gaactcccct ggttcaatca ccggtttgcc atcaagcccg ataaagctca tctcttcccc
     5581 gctcagggtg aagtagaccg tcttgctctc gcccggtgcg agctcgatct tgcggaagcc
     5641 cttaagccgc ttgttgggcg gggcgatcga ggcgaccaga tcgctgagat agagctggac
     5701 cgtctccaga ccggcaacct ggccggtgtt ggtcactgtg acgctggcgg tgatcacctc
     5761 accccgcgcc gcgctgctct tgtccagttt gagatcgctg taggcgaagg tggtgtagct
     5821 caagccgaag ccgaacggcc actggggatt gaagcgattg gtgccgccgg tgttttcagt
     5881 ccagagatga tcataaagca ccagatcgtt gggcgatcgc ggataggtga cgggcagtct
     5941 gcccgagggg ttggcgtcgc cgaagagcac atcggcgatg gcgcgccctc cctccattcc
     6001 cggcagccag gcgaggagga tggcgcgggc gccatccacg atcggacgga tgatccgcgg
     6061 ccgcccctgg agcagcacca ccacaaccgg cttgccgctt ttctgcagag ccaggaccag
     6121 gtcgagctgg
//
LOCUS       DAOPWU010000199         6126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12787_length_6126_cov_5.423159, whole genome shotgun sequence.
ACCESSION   DAOPWU010000199 DAOPWU010000000
VERSION     DAOPWU010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12787_length_6126_cov_5.423159"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..764
                     /gene="pyrH"
                     /locus_tag="PKM23_10200"
     CDS             33..764
                     /gene="pyrH"
                     /locus_tag="PKM23_10200"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNY91885.1"
                     /translation="MSDPGKSVYRRILLKLSGEALMGDSNRLGIDPQTVDQICREVAE
                     VARMGVEIGVIIGGGNIFRGLSASARGMDRVTGDYMGMLATVINALALQDYLERNGAE
                     TRVMTAIKMEQIAEPFIRRRAVTHLAHGKIVIFGAGTGNPYFTTDTAAALRAIEIGAD
                     AILKGTKVDGVYDKDPVLHADAVKYDQLTYMDVIRGRLKVMDTTAVTLCMENNLPIQV
                     FNLKEPGNLLRVIQGESIGTKVTGD"
     gene            768..1325
                     /gene="frr"
                     /locus_tag="PKM23_10205"
     CDS             768..1325
                     /gene="frr"
                     /locus_tag="PKM23_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNY91886.1"
                     /translation="MIEEIKKDVHHRMDAAVESVRSELAKLRTGKASPAILDGIVVNY
                     YGTPTPLRQAANVSAPEPRLLVIQPWDRSLLGEIEKAILKSDLGLNPANDGIVVRIPI
                     PQLTEERRVSLVKVAKRVGEEGRVAVRNVRRDGIERLKKLEKDSKVSEDDSRRAQEEV
                     QHMTDELVKKIDEILVLKEKEIMEV"
     gene            1413..2177
                     /locus_tag="PKM23_10210"
     CDS             1413..2177
                     /locus_tag="PKM23_10210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY91887.1"
                     /translation="MPVVYDRFARFYDLEYSLKEDDLPFYLDLADRFGDPVLEIGAGT
                     GRITLELARAGHEVCGIDDSARMLAIAGRKLRKLPEGAGPVELIRADMRDFGLKKKFA
                     LCIIPFRAFLHNLTQADQLATLAAIHRALRPGGYLAFDLFVPLHQVLSHRDWHEEISA
                     EELADPEQGISLTVDIDHDPARQLLSIRNTYHQADGKESSAVMRYRYIYRYEMEALLA
                     LSGFTVEAVYGGFSEEPYDYHSGVMCFVARKSGRRR"
     gene            complement(2174..2887)
                     /locus_tag="PKM23_10215"
     CDS             complement(2174..2887)
                     /locus_tag="PKM23_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNY91888.1"
                     /translation="MHEHVDPVHWERLAVSRIVPWEVAKRHPGAGEEVERQPGMNVAL
                     SRTHGAGGDSVAEALGQLIQWPVYDRQLVDFIAENARVRDRVVATLDEKKQSEMENWV
                     QTLFDRSALATDKYLKHLLKVLTSIFSHGQAIVVGRGGHFVADPRSTLRVLVTAPLPW
                     RVQAIMAKRGVDKKAAQKIVTEVDKSRAAFIKRYFHKDPDDLAAFDLVINSEQTTPDQ
                     AAQILRCALEQRTGRPVAP"
     gene            complement(2976..3401)
                     /locus_tag="PKM23_10220"
     CDS             complement(2976..3401)
                     /locus_tag="PKM23_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91889.1"
                     /translation="MIRRCAIPLFLLCAALLNTGCKNPLSEEKNKKLVHEAVFYDAPM
                     GRYVYFWNGRDLQNRYVTPGKYYFVLEVKDFQDQDYVTAVEGGKQSDAESSYYYYNEI
                     WHTNELGKIEPNPFPIMAGCTVTFILNGSAAAKLSIYQD"
     gene            complement(3422..5473)
                     /locus_tag="PKM23_10225"
     CDS             complement(3422..5473)
                     /locus_tag="PKM23_10225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNY91890.1"
                     /translation="MKKIIPLLLLSALITLPLIAADRRAMTADDLWAMKRIGDLALSP
                     DGRWIAFSQTCYDMQKNSGNSDLWIMPAGGGEPRQLTLHPQYDGAPQWLPDGSTLSFL
                     SSRSGSNQLYCLPLEGGEARPVTSLPVDVEDYLWSADGKQLLFIATVYPDAPTLEESA
                     ARVEKREESPVKARTSEQLFFRSFNHYREGMRSHLFLCNADGSGLRDLTPGEYDTPPL
                     DLGGARDFALSPDGGEVAYVRNIDPMPAAQTNNDLFLLSLKGGEPRCITTANKAVDNH
                     PLYSPDGRWIAYKAMSRPGFEADQEELMLYDRASGRSRSLTASFDSKIGEMIWAPDGS
                     SLYLTAAHQARECIFRVDVATAAVTEILHQHVNSAITISPDGRTLYFKQQSATAPDDI
                     HSLDLASGQVRQLTAVNQPLLDQLALNPAEEFWFTSFDGKKVHGMLVKPPFFDPAKKY
                     PMIYLIHGGPQGAFEDGFHYRWNPALFAAPGYVAVFVNFRGSTGYGQAWTDAVSRDWG
                     GGPYKDLMAGIDYLLKTFPFIDGERMAAAGGSYGGFMVNWIAAHTSRFKALVTHAGVF
                     DQISMYGATEELWFPEWEFGGVPYQKPELFKKWSVSSCVDNFKKFRTPTLVIHGEKDY
                     RVPYTQGLQMFTALQRMGVPSRILLYPDETHFVLKPQNARLWWQEVHGWIGRWTGSSQ
                     D"
     gene            complement(5498..>6126)
                     /locus_tag="PKM23_10230"
     CDS             complement(5498..>6126)
                     /locus_tag="PKM23_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY91891.1"
                     /translation="FIASRPEDKARNVPLDAAITLAFSEAMDQPLAGLAFALSDTGRT
                     PLPGRITWLSSAEMEFRPDSLLRPDRLYTVRMAVDSLRDRQGNPLADTLLTIRFQTLN
                     PDTLSEIAGALRDAAPGRSGPFFITARVPKGAAYEITAAQEGAWRFEKIMPGEYLIDF
                     FRDEDGDGRFSPGRPFPWQPAERYTLYPDTIRVRSRWPNEGNDLLLPE"
BASE COUNT         1112 a         1729 c         2124 g         1161 t
ORIGIN      
        1 cttttttttg aaggtcctga aggagggtgc gcatgagcga tccgggcaaa tccgtctacc
       61 gtcgcatctt gctcaagctg agcggagagg ccctcatggg cgacagcaac cggctgggca
      121 tcgatcccca gacggtcgac cagatctgcc gtgaggtggc cgaggtcgcg cgcatggggg
      181 tggagatcgg tgtcatcatc ggcggaggca acatcttccg cggactctcc gccagtgctc
      241 gcggcatgga tcgcgtcacc ggcgactata tggggatgct ggccacggtg atcaatgccc
      301 tcgccctgca ggattatctg gagcgcaacg gcgccgagac gcgggtgatg acggcgatca
      361 agatggagca gatcgccgag cccttcatcc ggcggcgggc ggtcacccat ctcgcccacg
      421 gcaagattgt catcttcggt gcgggcaccg gcaaccccta tttcaccact gatacggccg
      481 ccgccctgcg cgccatcgag atcggcgccg acgccatcct caagggcacc aaggtcgacg
      541 gggtctacga caaggatccg gtcctccacg ccgatgccgt caagtatgat cagttgactt
      601 atatggatgt gatccgcggc cggctcaagg tgatggacac cacggcggtt accctctgca
      661 tggagaacaa tttgccgatt caggtgttta atctcaagga gcccgggaat cttttacgtg
      721 tgatccaggg tgaatccatc ggaaccaaag tgacaggtga ttagcctatg atagaagaaa
      781 tcaagaaaga tgtccaccac cgcatggatg cggccgtgga gagcgtccgc agcgagctgg
      841 ccaagctgcg cacgggcaag gcctcgcccg cgatcctgga cggcatcgtc gtcaattact
      901 acggtacgcc cacgcccctg cgccaggcgg ccaacgtcag cgcgcccgag ccgcgcctgc
      961 tggtgatcca gccctgggac cgttccctgc tgggtgagat cgaaaaggcg atcctcaaat
     1021 ccgacctcgg cctcaatccg gccaatgacg gcatcgtggt gcgcatcccg atcccgcagc
     1081 tgaccgagga gcgccgcgtc agcctggtca aggtggccaa gcgtgtgggc gaagagggcc
     1141 gggtggcggt gcgcaatgtg cgccgcgatg gcatcgagcg tctgaaaaag ctggagaagg
     1201 attccaaggt ctccgaggat gactcgcgcc gcgcccagga ggaggtccag cacatgaccg
     1261 acgagctggt caaaaagatc gacgagatcc tcgtgctgaa ggaaaaggag atcatggagg
     1321 tctgagcggc ggggccgcac cggaccggtc atgcaggatt cacatgggac cgcggcagca
     1381 ccagcgccgc ggttttttta tggaggtgtg cgatgcccgt cgtctacgac cgttttgccc
     1441 gattttacga ccttgagtac agtctcaagg aggatgacct gcccttctac ctcgatttgg
     1501 ccgaccggtt cggcgatccg gtcctggaga tcggggccgg caccgggcgg atcaccctcg
     1561 agctcgcccg ggccgggcat gaggtctgcg gcatcgatga ttcggcccgc atgctcgcca
     1621 tcgccgggcg caaactgcgc aagctgccgg agggggcagg cccggtggag ctgatccggg
     1681 ccgatatgcg cgatttcggg ctcaagaaaa aatttgcact ctgcattatc ccctttcgcg
     1741 cctttctgca caatctcacc caggccgatc agctcgccac cctcgccgcc attcaccgcg
     1801 ccctgcggcc gggcggctat cttgcctttg atctcttcgt gccgctccat caggtcctca
     1861 gccaccgcga ctggcatgag gagatttccg ccgaggagct ggccgacccg gagcagggta
     1921 tatccctgac cgtggatatc gatcacgatc cagcccggca gctgctctcc atccgcaaca
     1981 cctatcacca ggcagatggg aaggagagca gcgcggtcat gcgctaccgc tacatctacc
     2041 gctatgagat ggaggccctt ctcgccctga gcggttttac cgtcgaggcg gtatatggcg
     2101 gattcagcga agagccctac gactatcaca gcggtgtgat gtgcttcgtc gcccgcaaga
     2161 gcggacgacg gcgctagggg gcgacggggc gcccggtgcg ctgctccaga gcgcagcgga
     2221 ggatttgtgc agcctggtcc ggcgtggtct gctcgctgtt gatcaccagg tcgaaggcgg
     2281 ccagatcgtc cggatccttg tggaaatagc gtttgatgaa agcggcgcgg gatttgtcca
     2341 cttcggtgac gatcttttgc gccgcttttt tatccacccc tcgtttggcc atgatcgcct
     2401 gcacccgcca gggcagtggg gcggtgacca ggacgcgcag ggttgagcgt ggatcggcga
     2461 caaagtgacc accgcggccc accacaatag cctggccatg gctaaagatg gaggtcagga
     2521 ccttgagcag gtgtttgagg tatttgtccg tggccagggc gctgcggtcg aagagggttt
     2581 gcacccagtt ctccatctcg ctctgcttct tctcatcgag ggtggccacc acccgatccc
     2641 gcacccgtgc attttccgcg atgaaatcga ccagctgccg gtcgtagacc ggccactgaa
     2701 tgagctgccc cagggcctcc gcgacgctgt cgccgccagc gccgtgggtg cgcgacaggg
     2761 ccacattcat cccgggctgc cgttccacct cttcgccggc cccgggatgg cgtttggcca
     2821 cctcccaggg gacgatgcgg ctgacggcca gccgttccca atggacggga tcgacatgtt
     2881 catgcatggc tgcctcccgt ggttaggatt caacagacct atacttgagt ataagctttc
     2941 gctggctaaa agtcaagggg tataggcgga gcggatcagt cctggtagat ggaaagtttg
     3001 gcggctgcac tgccgttgag gatgaaggtg acggtgcagc cggccataat ggggaaggga
     3061 ttgggctcga tcttgcccaa ttcgttggta tgccagattt cgttgtagta atagtaggag
     3121 gattcggcgt cgctctgttt gcccccctcc accgcagtga cataatcctg atcctggaaa
     3181 tccttgacct cgaggacaaa gtagtatttt ccgggcgtca catagcggtt ctgcaggtcg
     3241 cggccgttcc agaaatagac gtagcggccc atgggggcat cgtagaaaac ggcctcatgg
     3301 acgagctttt tgttcttttc ctccgagagg ggattcttgc agccggtgtt gagcagggcg
     3361 gcgcagagga ggaaaagagg gatggcgcat ctgcggatca tgttttcgag cctgaaaagg
     3421 ttcagtcctg cgaactcccg gtccaccgtc cgatccagcc gtgcacctct tgccaccaga
     3481 ggcgtgcgtt ttggggcttg agcacgaaat gggtctcgtc cggatagagc aggatgcgcg
     3541 aggggacgcc catgcgctgg agggcggtga acatctgcag cccctgtgta tagggcacgc
     3601 ggtagtcctt ctcgccgtgg atgaccagag tcggggtgcg gaattttttg aaattgtcca
     3661 cacaggatga aacagaccat tttttaaaaa gttccggctt ttggtagggc accccgccga
     3721 actcccattc ggggaaccag agttcttcgg tggcgccgta catgctgatc tggtcgaaga
     3781 ccccggcatg ggtgaccagg gccttgaagc ggctggtgtg ggctgcgatc cagttgacca
     3841 taaagccgcc gtagctgccg cccgccgcgg ccatgcgctc gccgtcgatg aagggaaagg
     3901 ttttgagaag ataatcgatg ccggccatga ggtccttgta gggtccgccg ccccagtccc
     3961 ggctcaccgc atcggtccag gcctggccgt agccggtcga gccgcggaaa ttgacaaaga
     4021 ccgccacata gccgggcgcg gcgaagaggg ccggattcca gcggtagtga aagccgtcct
     4081 caaaggcccc ctgcgggccg ccatggatca gatagatcat ggggtatttt ttcgcgggat
     4141 cgaaaaaggg cggcttgacg agcatgccgt ggaccttttt cccgtcaaag ctggtgaacc
     4201 agaactcctc ggcgggattg agggcgagct ggtcgaggag gggctggttg acagcggtga
     4261 gctgccgcac ttgaccgctg gcgaggtcga ggctgtggat atcgtccggc gcggttgcgg
     4321 actgctgttt gaaataaagg gtccggccgt ccggggagat ggtgatggcg ctgttgacgt
     4381 gctggtgcag gatctcggtc acggcggcgg tggccacgtc aacgcggaag atgcattcgc
     4441 gcgcctgatg ggcggcagtg aggtagaggc tgctgccgtc cggggcccag atcatctcgc
     4501 cgattttgct gtcaaaggag gcggtcaggc tgcggctccg gccgctggcg cggtcgtaaa
     4561 gcatgagctc ctcctggtcg gcctcgaaac ccgggcggct catcgccttg taggcgatcc
     4621 agcggccgtc gggcgagtag aggggatggt tgtcgaccgc cttgttggct gtggtgatgc
     4681 agcgcggctc gcctcccttg agggagagga ggaagaggtc gttgttggtc tgggctgcgg
     4741 gcatggggtc gatgttgcgc acataggcga cctcgccgcc gtcgggggag agggcgaaat
     4801 cgcgcgcccc gccaagatcg agaggcggcg tatcatattc gccgggggtg aggtcgcgga
     4861 ggccgctgcc gtcggcgttg cagaggaaga ggtggctgcg catgccctcg cggtaatggt
     4921 tgaaggagcg gaaaaagagc tgctcgctgg tgcgggcctt gaccgggctc tcttcccgct
     4981 tttcgacacg ggcggcgctc tcctccaggg tcggggcatc aggatagacc gtggcgatga
     5041 agaggagctg tttgccgtcg gctgaccaga gatagtcctc gacatcgacc ggcagggagg
     5101 tgacggggcg cgcctcgccg ccctccaggg gcaggcagta gagctggttg gagccgctgc
     5161 ggctggagag gaagctgagg gtggagccgt cggggagcca ctgcggggcg ccgtcgtact
     5221 ggggatggag ggtgagctga cggggctctc cgcccccggc cggcatgatc cagaggtcgg
     5281 agttgccgct gtttttttgc atatcatagc aggtctggct gaaggcgatc cagcggccgt
     5341 caggagagag ggcgaggtcg ccgatccgtt tcatcgccca gaggtcgtca gcagtcatcg
     5401 cgcgccggtc ggcggcgatg aggggaaggg tgatgagggc ggagaggagg agcagcggga
     5461 tgattttttt catgggaact ccggttagat gggtcgatta ttcggggagg aggaggtcat
     5521 tgccttcgtt gggccagcgc gagcgcaccc tgatggtatc cggatagagg gtatagcgtt
     5581 cggcgggctg ccaggggaag gggcggccgg gggaaaagcg gccgtcgccg tcctcgtcgc
     5641 ggaaaaagtc gatcagatac tccccgggca tgatcttttc aaagcgccag gccccttctt
     5701 gagcggcggt gatttcatat gctgctccct tggggacgcg ggcggtgatg aaaaaggggc
     5761 cggaacggcc gggcgcggcg tcgcgcaggg cgccggcgat ctccgagagg gtgtccggat
     5821 tgagggtctg gaaccggatg gtgaggaggg tgtcggcgag cgggttgccc tggcggtcgc
     5881 gcagcgagtc cacggccatg cggacagtgt agaggcggtc cgggcggagg agggaatcgg
     5941 gccggaactc catttcggcg ctgctgagcc aggtgatgcg gccgggcagt ggggttcgcc
     6001 cggtatcgct gagggcgaag gccagcccgg ccaggggttg atccatggcc tccgagaagg
     6061 cgagggtgat ggcggcgtcg agcgggacgt tgcgcgcctt gtcctcgggc cgggaggcga
     6121 tgaaat
//
LOCUS       DAOPWU010000200         6098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12862_length_6098_cov_4.858845, whole genome shotgun sequence.
ACCESSION   DAOPWU010000200 DAOPWU010000000
VERSION     DAOPWU010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12862_length_6098_cov_4.858845"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="PKM23_10235"
     CDS             <1..1773
                     /locus_tag="PKM23_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HNY91892.1"
                     /translation="DGGAPVTARGVCWSTVTGPTTADAKTSDGAGSGAFASKVTGLNA
                     GATYYLRAWATNDAGTAYGEEMTFKTLDSLVVPTVTTVAVNNIMVKSAKSGGNVTEWG
                     GSDVIARGICWNTTGDPTIADEKSENGAGIGSFTSSLSALTATTTYYVRAWATNASGT
                     GYGQVETFTTQSPAPAVLKVVAQDGSGDYTTVQAAFNDVPDFYTGMWCIFVKSGTYKE
                     KLMLAQNKTNVVLKGENVMTTILTYDDYAGKPGIGGTSNCYSTAIEADDFTAMDITFQ
                     NTVKNDGSFADQQAVALRVNGDRGAYYNCRLLGYQDTYYAWGGHGTVRTYMKDCYIEG
                     SVDFIFGRNINVFDHCTLHVNRQNGCITAPSTDATSRFGLVFMDCVITADSVGFNDVP
                     ITSFHLGRPWQGRPQCVYLRCEEPATLSPEGWTVMSTGLNPLFAEYHCFGAGSNFFGR
                     INGGIQLSDAEAAEYTLANIFSKTSSPNLGFDWMPEKPIFTDVEWSKGPESLPSVYEL
                     SQNYPNPFNPVTTINYALPKAGKVRITIYNILGAQVATLVDRQQAAGRYTVRFDGLQL
                     GSGTYFYRIDAGEFHQTKKMLLIK"
     gene            1846..3087
                     /locus_tag="PKM23_10240"
     CDS             1846..3087
                     /locus_tag="PKM23_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012764307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNY91893.1"
                     /translation="MTRLRMIFLMAALAGSTWGQGTLLPDASFQGIGAGSAPDLPSQH
                     TGGTPAPNTTFQHIGVTADLPVFYAEVKQTVTFPGSWLAASGRNFTEWRSATRQIVFD
                     HLLAEPPRASFQPVTLAEEDRGSYTARKVVFNLNGWSRVLGYLLVPKGEGPFPAVLLL
                     HDHGARFDIGKEKMVRPLQVDPARLQAAEEWVRGGYGGRFVGDELARRGYLCLAVDAL
                     NWGDRGGAGKEGQQALASNLFNLGISLAGLVAWEDLAALRFLSSLPEADTTRIAAIGH
                     SFGSYRAWQLAALSEKIRAAVCICWMAGRMELMVPGNNLTKGQSSYTTLHPGLTRLLD
                     YPDVASLACPRALLFFNGEDDHLFPQPAARAAFDKMRRIWEEQGAGDRLVTRFWPGGH
                     QFSREMQEAAFDWLDGQLGRR"
     gene            3112..5655
                     /gene="pelA"
                     /locus_tag="PKM23_10245"
     CDS             3112..5655
                     /gene="pelA"
                     /locus_tag="PKM23_10245"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HNY91894.1"
                     /translation="MTRFAYLAGVGLLLALTLLPGLSPAIAAGGEGFEEAIQPPVIPE
                     RSLSIRDFGAISDGHTLCTEAIQRTITTCAGAGGGRVIIPSGIWLTGPIRLESRIELH
                     LESGALLLFSSDHSLYPIIQAPTKGWLVASPIYGYKLEDVAVTGRGIIDGAGESWRPV
                     KKFKTTSRQWKELVDSGGIVSEKGDMWWPSREALEGEAWLEKLQKSKPKKEHTAEDYL
                     PARDYLRPYMLSFIDCKRVFFEGVTIRNSPKFALCPAWCDQLIIRDVKVNNEWWAQNG
                     DGIDISACRNVLVENCTVTAGDDGICMKSSDRSGRTGPALENVLIRNCIVYHGHGGFV
                     VGSNTDGGMRNICVENCTFIGTDAGLRFKSARGRGGVVENIRIRNIRMKDITGEAISF
                     DAFYESSQPDTLARAVTPETPVFRNITIDSLYCAGAARAIVMTGLPEMPVQNIRIRDA
                     HISATAGVQLRDVRDLALERVVVKPARGALYDFKRCSGISIDGAGFMDKSADFPSDSW
                     RSLMHHDNAWFATPDAAAVAENLLLYQCAIGGWPKNIDMARPLSPAERAALASPPADT
                     LATIDNGATWTQLTFLARLISAHPEPRFIAAFNRGLDYLFAAQYDNGGWPQFYPLHPG
                     YYSHITYNDDAMIGVLTLLHGIAANEERFRFVDAARRARAASAVERGIGCILATQLKE
                     GERRTAWCAQYDEKSLQPAPARRYELVSLSGDESVGIVRFLMRLRDPAPEIVDAVQGA
                     VDWLERVKIIGTRVDYVTDASLPGGFNKVVVSDPAAPPIWARFYLIGSNRPFFSDRDG
                     RIYYDLAEISAERRNDYAWLGYWPQELLAKEYPAWLARADVRLKLSGVK"
     gene            complement(5743..>6098)
                     /locus_tag="PKM23_10250"
     CDS             complement(5743..>6098)
                     /locus_tag="PKM23_10250"
                     /inference="COORDINATES: protein motif:HMM:NF037003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HNY91895.1"
                     /translation="EILTNREAIAVDQDSLGIQGLKYLAEDSLEVWFKPLTGGEWAAC
                     FLNRSRLPKKVLFDWKSHKIEDSFSGRAIDFSTSQWRIRDLWAHREIGTTRKPFSAEI
                     PSHDVRMLRLQPARK"
BASE COUNT         1146 a         2051 c         1780 g         1121 t
ORIGIN      
        1 gacggtggcg ccccggtgac cgcgcgcggg gtctgctgga gcacagtaac gggtccgacc
       61 accgccgatg ccaaaaccag cgacggagcc ggctcgggcg cctttgccag caaggtgacc
      121 ggcctcaatg ccggcgcaac ctactatctc cgcgcctggg ccaccaacga cgccggcacc
      181 gcctacggcg aagagatgac ctttaagacc ctcgattctc tggtggtccc gacggtgacg
      241 acggtcgcgg tcaacaacat catggtcaaa tcggccaaga gcggcggcaa tgtgacggag
      301 tggggcggaa gcgatgtcat cgcccgcggc atctgctgga ataccaccgg tgatcccacc
      361 atcgccgacg aaaagagtga gaacggcgcc ggcattggct ccttcaccag cagcctctcg
      421 gccctgactg ccaccaccac ctactatgtg cgcgcctggg cgaccaacgc cagcggcacc
      481 ggctacggcc aggttgagac cttcaccacc cagtcgcccg ctccggcggt cctcaaggtc
      541 gtcgcccagg atggctcggg cgactatacc accgtccagg ccgccttcaa cgacgtgccc
      601 gatttctaca ccgggatgtg gtgcatcttc gtcaagtccg gcacctacaa ggaaaagctc
      661 atgctcgccc agaacaagac caacgtcgtg ctcaagggcg agaatgtcat gaccactatc
      721 ctcacctacg acgattatgc cggcaagccg ggcatcggcg gcacctcgaa ctgctacagc
      781 accgccatcg aggcggatga tttcaccgcc atggatatca ccttccagaa taccgtcaag
      841 aacgatggct ccttcgctga ccagcaggct gtcgccctgc gcgtcaacgg cgaccgcggc
      901 gcctattata actgccgcct cctcggctac caggatacct attacgcctg gggcggccac
      961 ggcaccgtac gcacctacat gaaggattgc tacattgagg gctcggtcga tttcatcttc
     1021 ggccgcaata tcaacgtctt cgaccactgc accctccacg tcaaccgcca gaacggctgc
     1081 atcaccgcgc ccagcaccga tgccacctcc cgcttcggtc tggtcttcat ggactgcgtg
     1141 atcaccgccg attcggttgg tttcaatgat gtaccgatca cctccttcca cctcggacgg
     1201 ccgtggcagg ggagaccgca gtgcgtctat ctccgctgcg aagagcccgc caccctcagt
     1261 cccgaaggct ggacagtgat gagcaccggc ctcaatcccc tcttcgccga atatcactgc
     1321 ttcggcgcag ggtcgaattt cttcggacgc atcaacggcg gcatccagct cagcgatgcg
     1381 gaagcggccg agtataccct cgccaacatc ttttccaaaa ccagcagccc caacctgggc
     1441 ttcgactgga tgccggaaaa gccgatcttt acggatgttg aatggagcaa gggaccggag
     1501 agcctgccgt cggtctatga actctcgcag aactatccca atcccttcaa tccggtcacg
     1561 accatcaact acgccctgcc caaggcgggc aaggtgcgga tcaccatcta taacatcctc
     1621 ggggcgcagg tcgcgaccct ggtcgatcgc cagcaggccg ccggacgcta taccgtccgc
     1681 ttcgacggcc tccagctcgg cagcggcacc tatttctacc gcatcgatgc cggggaattc
     1741 caccagacca aaaagatgct gctcatcaag tagccgggca gcctgacaac ctgacctaag
     1801 gaaaccgggc gcagccgggt cacaacggct gcgcccggaa gtataatgac tcgtttacga
     1861 atgatctttt taatggccgc cctcgccggg agtacctggg gtcagggcac cctgttgccg
     1921 gatgcctcat ttcagggcat aggcgcaggt tccgctccag atctccccag tcagcacacc
     1981 ggtggaacac ccgcccctaa taccactttt cagcacatcg gtgtaaccgc agatcttccg
     2041 gtcttttacg ccgaggtcaa acagacggtc accttccccg gctcctggct ggcggcatca
     2101 ggcagaaatt ttacggaatg gcgcagcgcc acacggcaaa tcgtcttcga ccacctcctc
     2161 gccgagccgc cgcgggcatc cttccagccg gtaaccctcg ctgaagagga ccggggcagc
     2221 tacaccgccc gcaaggtcgt tttcaatctc aatggctgga gccgggtcct tggctatctg
     2281 ctggtgccca aaggagaggg gcccttcccg gccgtgctgc tcctccatga ccacggcgcg
     2341 agattcgaca tcggcaagga aaagatggtt cgccctctcc aggtcgatcc ggccaggctg
     2401 caggctgcgg aagagtgggt ccggggcggc tacggcgggc gcttcgtcgg cgatgaactg
     2461 gcccggcgcg gctacctctg cctggccgtc gacgccctca actggggcga ccgcggcggc
     2521 gccggcaagg agggacagca ggccctcgcc tccaacctct tcaatcttgg catctccctg
     2581 gccggcctgg tcgcctggga ggacctcgcc gccctgcgtt ttctctcctc cctgcctgaa
     2641 gcggatacga cccgcatcgc cgcgatcggc cattccttcg gctcctaccg cgcctggcag
     2701 ctggccgccc tttcggagaa gatcagggcg gcggtctgta tctgctggat ggccgggcgc
     2761 atggagctga tggtgccggg caacaacctc accaaaggcc aatcctccta caccaccctg
     2821 caccccggcc tgacccggct gctcgactat cccgacgtcg ccagcctcgc ctgcccgcgg
     2881 gccctgcttt tcttcaacgg cgaggatgac catctttttc cccagcccgc cgcccgggcc
     2941 gccttcgaca agatgcggcg catctgggag gagcagggcg ccggcgacag gctggtcacc
     3001 cgcttctggc ccggcggcca ccagttctcc cgggagatgc aggaggccgc ctttgactgg
     3061 ctcgatggcc agctcggacg ccgctagtcc caatttaaca ggagtaacac cgtgaccaga
     3121 tttgcatacc tcgccggagt ggggctcttg ctggccctga ctcttttacc aggcctcagt
     3181 cccgcgatcg cagcgggtgg agagggcttt gaagaggcca tccagcctcc cgtcatcccg
     3241 gagcgcagcc tttccatccg cgatttcggc gccataagcg acggccatac cctctgcacc
     3301 gaggccatcc agcggactat caccacctgc gccggggccg gcggtggcag ggtgatcatc
     3361 ccttcaggga tctggctcac cggcccgatc cgcctcgaaa gccgcattga actccacctc
     3421 gagagcggcg cgctgctcct tttctccagc gatcacagcc tctatcccat cattcaggcc
     3481 ccgaccaagg ggtggctggt cgcctcgccc atttatggct acaagctgga ggatgtcgcc
     3541 gtcaccggcc gcggcatcat cgacggggcg ggggagagct ggcggccggt gaagaaattc
     3601 aagaccacct cccggcagtg gaaggaactc gtcgattccg gcggaatcgt cagcgaaaag
     3661 ggggatatgt ggtggccttc acgggaagcg ctggaggggg aggcctggct cgaaaagctg
     3721 cagaagagca aaccgaaaaa ggagcacacc gcagaagatt atctcccggc gcgggattat
     3781 ctccgtccct acatgctctc tttcatcgac tgcaagcggg tgttctttga gggcgtgacg
     3841 atcaggaact cgcccaagtt tgccctctgt cccgcctggt gcgatcagct gatcatccgg
     3901 gatgttaaag tgaataatga atggtgggcg cagaacggag acggcatcga catctcggct
     3961 tgccgcaacg tgctggttga aaactgtacg gtaactgccg gagatgacgg tatttgcatg
     4021 aagtccagcg atcgctcggg gcgcacaggt ccggccctgg agaatgtcct catccgcaac
     4081 tgcatcgtct accacggcca cggcggcttt gtcgtcggca gcaacaccga cggcggcatg
     4141 cgcaatatat gcgtcgagaa ctgcaccttc atcggcaccg atgcaggcct gcgcttcaag
     4201 agcgcgcgcg gacgcggggg tgtcgtcgaa aacatccgca tccgcaatat ccgcatgaag
     4261 gatatcaccg gcgaggcgat cagctttgac gccttttacg agagcagcca gccggacacc
     4321 ctggcccgcg ctgtcacccc ggagaccccc gtcttccgca atatcaccat cgacagcctt
     4381 tactgcgccg gcgctgcccg ggccatcgtc atgaccggct tgcccgagat gcctgttcag
     4441 aacatccgca tccgcgatgc ccacatctcc gccactgccg gcgtgcagct gcgggatgtc
     4501 cgcgacctcg cccttgaacg ggtggtggtc aaaccggcgc ggggtgcgct ctacgatttc
     4561 aagcgctgca gcggcatctc catcgacggc gccggcttta tggacaagag cgccgacttc
     4621 ccttcggaca gctggcgctc cctgatgcac catgacaatg cctggtttgc caccccggat
     4681 gcggccgccg tcgcggagaa cctcctgctc taccagtgcg ccatcggcgg ctggcccaag
     4741 aacatcgata tggcccgccc cctgagtccg gcggagaggg ccgccctcgc ctcgccaccc
     4801 gccgacaccc tcgccaccat cgataacggc gccacctgga cccagctcac cttcctggca
     4861 cgcctcatct cagctcaccc tgagccgcga ttcatcgctg ccttcaaccg cgggctcgac
     4921 tatctgtttg cggcccagta cgacaacggc ggctggccgc agttctatcc actccatccc
     4981 ggctactatt cgcatatcac ctacaatgac gatgccatga tcggggtact caccctgctg
     5041 catggaatcg cagcgaatga ggagcgcttc cgtttcgtcg atgccgcgcg gcgggctcgg
     5101 gcggcctctg cagtcgagcg tggtattggc tgcattcttg caacccagct taaggaaggg
     5161 gagaggcgga cggcctggtg tgcccagtat gacgagaaga gcctgcagcc ggcccccgcg
     5221 cgcagatacg agctggtttc gctcagcggc gacgagagcg tcggcattgt ccgctttctc
     5281 atgcgcctgc gcgatcccgc gccggagatc gtcgacgcgg ttcagggtgc ggttgattgg
     5341 ctggagaggg tcaaaataat aggcacccgc gttgattacg tcacggatgc ctctttgccc
     5401 gggggcttta ataaggtggt ggtgtccgat cccgccgcac caccgatatg ggcccgcttt
     5461 tacctgatcg gctcgaaccg gccttttttc agcgatcgcg acggccggat ctattacgat
     5521 ctggccgaga tctccgccga acgccgcaac gattatgcct ggctgggtta ctggccccag
     5581 gaactgctgg caaaagagta ccccgcctgg ctggcgcggg cggatgtgcg cctgaaactg
     5641 agcggggtaa agtaatccac acctgtcggc tgctgttttt ttccccaccc tgactgagga
     5701 cagctgcagc gcctcggctg cggctgtccc cgccccggca gcctatttcc gcgccggctg
     5761 gagcctcagc atgcgcacat catgcgacgg gatttccgcc gagaagggtt tgcgggtggt
     5821 gccgatctcg cgatgcgccc agagatcacg gatccgccat tgtgatgtgc tgaaatcgat
     5881 agcccggccg gaaaagctgt cctcgatctt gtggctcttc cagtcgaaaa gaaccttttt
     5941 cggcagccgg ctgcgattga gaaagcaggc cgcccattcc ccgccggtaa ggggtttgaa
     6001 ccagacctcc aggctgtcct cggcgaggta tttgagcccc tgaatgccga gggaatcctg
     6061 gtcgacggcg atggcctcgc ggttggtgag gatctcga
//
LOCUS       DAOPWU010000201         6057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_12965_length_6057_cov_5.444518, whole genome shotgun sequence.
ACCESSION   DAOPWU010000201 DAOPWU010000000
VERSION     DAOPWU010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12965_length_6057_cov_5.444518"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..887
                     /gene="trmB"
                     /locus_tag="PKM23_10255"
     CDS             234..887
                     /gene="trmB"
                     /locus_tag="PKM23_10255"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNY91896.1"
                     /translation="MAKQKLIRFAALSSLANVRQEPDGLRGRWAADFFHNDHPLILEL
                     GCGRGDYAVALAQGHPEINVLGLDIKGPRLWVGASRALELQLGNAGFVRTAIEQIETF
                     FAPGEIAAIWITFPDPHPPAGRRKKRLTSPRFLAIYRSLLQPGGLIHLKTDDEGLYYY
                     TLLTLRDEGAELLADLPDLYTGASNPDLTGIQTAYERRHLQEGKSIKYICFRFPTAG"
     gene            complement(957..1823)
                     /locus_tag="PKM23_10260"
     CDS             complement(957..1823)
                     /locus_tag="PKM23_10260"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNY91897.1"
                     /translation="MTYKKMSYISGIIIFFSAVILLWAILWLSGQRIISSSEYRLYIK
                     FADVVGLRDRSQVFMRGYRIGWTKGVRFEPDGVVVRVDIKDRFRIPVDSKIEINTLNL
                     IGEKAITIEPGTSTEMVPIEGQLSGENKDIMIVAKTMLTNIRDKMREGELDLRIKDAS
                     NSLKTLSAVLDKVDHKVDGLNIGMYNAQIAEIGEAARALRNAGTEAAELAKESRGSVK
                     KVDETAGKLSDLSDQLTGVAAKLNSGQGSAGELLNNKVYVENLNATVVELKLLIEDLK
                     KNPGKYVNVSVF"
     gene            complement(1813..2544)
                     /locus_tag="PKM23_10265"
     CDS             complement(1813..2544)
                     /locus_tag="PKM23_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY91898.1"
                     /translation="MISVRGIHKSFDEKEVLTGIDLDIPDNSTMVILGPSGQGKTVFI
                     KTLVRLIEPDAGSISYDGEDILRMSRREWQAYQNNIAFVFQNSALFDFLDVRENLSLF
                     LRMHKKLTPFQMYAEVSRAITFVGLGDDVLDKYPEELSGGMRKRVAIARAMIKRPKYI
                     FYDEPTTGLDQNNAEKVSELILMLKKEIAATSIIVTHDIKLMRDVADRVTLLREGNIS
                     FTGTKDEISAETLDFLYETRGEYDL"
     gene            complement(2541..3332)
                     /locus_tag="PKM23_10270"
     CDS             complement(2541..3332)
                     /locus_tag="PKM23_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY91899.1"
                     /translation="MARIPSEHFFEKLGERSISFFTTVYEIAILFMQTIRALPSLWFY
                     RRQFIEQLYAFSVKTLPIAAVIAIFIGLGATVQSTYQSSELLTRAVLVNVIFKTTILE
                     LCPIVLSLVLAGKLGASLAAEIGSMKISEQIEALETMSLDPVGFLVVPRVVAGLLMLP
                     VITIFANFLAIFSSFFVSAVTSEWISAAEFGSGLEMDFKVFEMYFGNLIKPAVYGVLI
                     ALMGSYFGLKTSGGAKGVGAASTNAVVVSAVFIVIFDYYLGKLLL"
     gene            complement(3345..4169)
                     /gene="mutM"
                     /locus_tag="PKM23_10275"
     CDS             complement(3345..4169)
                     /gene="mutM"
                     /locus_tag="PKM23_10275"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNY91900.1"
                     /translation="MPELPEVETICRSLAPHITGKVLAGVEVHAPKLRRPLPTAAELR
                     AVTGQRITALERRAKYLIWHLDGGGALIIHLGMSGRLCYHHGPHLAELHTHVIFHFQE
                     GGQLHFRDPRRFGSLDLAAPGELAGLPMLCELGVEPLSSAFTAEYFRERLGATRRVVK
                     TVIMDQRVVVGVGNIYANEALFQAGIHPLRPGQSLSPAEGEALQLAIICVLGKAVERG
                     GTTLSDYRNADGEPGFFQLDLAVYGREEEPCPRCGGQIVRLRHEGRSSYLCEACQK"
     gene            complement(4302..5414)
                     /locus_tag="PKM23_10280"
     CDS             complement(4302..5414)
                     /locus_tag="PKM23_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY91901.1"
                     /translation="MHIIKKEFIQVRRDRGMLMILFVAPILQLLLLGFVISSEVHNIR
                     TVICDLDNSSTSRLLVERIRNSGYFDLAFFDTRQNHLHDYLDNGRAVVAVVIPADFST
                     RLQRGEPAGLQVLMDGQDSNTSTIALGYINGILQSEIMDQIEGKLQGSEAAAEVHLIS
                     PNLRVWYNEELKNSDYMVPGIVVFLLTMVTTLVAAMGLVREREIGTLEQLLVAPLRKH
                     ELLIGKLVPFAVLGLIEVVFALCVAKLIYDIPIAGNLGLLFIFTLVYLFTTLGLGLFI
                     SATSGTQQQAMFLTLFFLMFFMLMSGFLFPIENMPRVMQGISLLNPMRYFVLVIREIF
                     IKSATVRHLYPQGLALLIFGGVVFSFAVLRFQSRMK"
     gene            complement(5431..>6057)
                     /locus_tag="PKM23_10285"
     CDS             complement(5431..>6057)
                     /locus_tag="PKM23_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY91902.1"
                     /translation="VWYNPDLKSVNFIVPGLVAVILMLICALLTSVTVARERETGTME
                     QILVSPVRSLEIIAGKTLPYILLGLLDGIVVVVFAWLWFGVPFRGEPLWLLGYALIFI
                     YTCLSFGIFVSARVPNQQMALMMTMVSTLLPAILLSGFIFPLASMPKLLQLLSYIVPA
                     KYFLVIIRSIMLKGIAGPLLWQPALFLVLFGTLLILISVKRFKINLEG"
BASE COUNT         1308 a         1793 c         1797 g         1159 t
ORIGIN      
        1 aacaaatagg ccgacccagg ctctccacac catgcccccc gtttccccgg ggggcttttt
       61 ttttaggctt ttccgcacca gactgcaggg ggacgcgcgc aaggtgtcgg gtgggatcca
      121 gatttcgggg ggtacgcgcg caaggtattg ggtgggatcc agatctcggg gggggtaccc
      181 cgctcgtaaa atctcttgat ttgctggtga aaatttcgta aatctccccc gtcatggcca
      241 aacaaaaact catccgcttc gccgccctct ccagtctcgc caacgtccgc caggagccgg
      301 acggcctgcg cggccgctgg gctgccgatt tttttcacaa cgatcatccc ctcatcctcg
      361 agctgggctg cgggcggggc gactatgccg tcgcccttgc acagggccat ccggagatca
      421 acgttctcgg gttggacatc aagggaccgc gcctctgggt cggcgccagc cgcgccctcg
      481 aactccagct cggcaacgcc ggctttgtcc gcaccgcgat cgaacagatt gaaacatttt
      541 tcgcccccgg cgaaatcgcc gcaatctgga tcacctttcc cgatccccat cccccggcag
      601 gacggcgcaa gaaacggctc acttcgccgc gctttctcgc gatctatcgc agcctgctcc
      661 agcccggcgg cctcatccac cttaaaaccg acgatgaagg actttactac tataccctcc
      721 tcaccttgcg tgatgaaggc gccgaactgc tcgccgatct gcccgacctc tacaccggcg
      781 cctccaatcc cgatctcacc gggatccaga ccgcctacga acgccgccac cttcaagaag
      841 gcaaatccat caaatatatt tgtttccgct ttcccaccgc cgggtaaata aaaagcgcag
      901 acgatctggc gatcctctgc gctcaagggg gggcgcccgg ccaaaaccgg ggcggtctaa
      961 aagaccgaga cgtttacgta cttgccgggg tttttcttca ggtcctcgat gaggagcttg
     1021 agttcgacca cggtggcgtt gaggttctcc acatagacct tgttgttgag cagctcgccg
     1081 gcggatccct ggccgctgtt gagtttggca gcgaccccgg tcagctggtc ggagaggtcg
     1141 gagagcttgc ccgcggtctc atcaaccttt ttgacactgc cgcggctctc cttcgccagt
     1201 tcggcggctt cagtgccggc attgcgcagg gcgcgggcgg cctcgccgat ctcggcgatc
     1261 tgggcgttgt acatgccgat attgaggccg tcgaccttgt ggtccacctt gtcgagcacg
     1321 gcactaaggg ttttgaggga gttggaggcg tccttgatgc gcaggtcgag ctccccctct
     1381 ctcatcttgt cgcggatatt ggtcagcatg gtcttggcga cgatcatgat gtccttgttt
     1441 tcaccgctga gctggccctc gatcggcacc atctcagtgg atgtgcccgg ttcaatggtg
     1501 atcgcctttt ccccgatcag gttgagggtg ttgatctcaa ttttggaatc gacggggatt
     1561 ctgaagcggt ccttgatgtc gacgcgcacg accacgccat ccggctcgaa gcgcacgccc
     1621 ttggtccagc cgatgcgata gccgcgcatg aagacctgcg agcggtcgcg gaggccgacg
     1681 acgtcggcga atttgatata gaggcggtac tcgctcgacg agatgatccg ctgtccggag
     1741 agccaaagaa tggcccacaa cagaatgacg gcactgaaaa agatgataat tccggaaata
     1801 tagctcatct ttttataggt catattctcc tcgcgtctcg taaagaaaat ccagggtttc
     1861 tgcagagatc tcatccttgg tgcctgtgaa ggagatgttg ccctcccgga gcagcgtcac
     1921 ccggtcggcg acatcgcgca tgagcttgat gtcgtgggtg acgatgatcg aggtggctgc
     1981 gatctccttc ttgagcatga ggataagctc actcaccttt tcggcattgt tttggtccag
     2041 tcccgtagtg ggctcgtcat aaaagatata tttgggccgt ttgatcatgg cccgggcgat
     2101 ggccacacgc ttgcgcatgc cgccgctgag ctcctcggga tatttgtcga gcacgtcatc
     2161 ccccaggcca acgaaggtaa tcgccctgct cacctcggcg tacatctgga agggggtcag
     2221 ttttttatgc atgcgcaaaa agaggctcag gttctcgcgc acatcgagaa aatcaaagag
     2281 ggcgctgttc tggaagacga aggcgatgtt gttctgatag gcctgccact ctctccggct
     2341 catcctgagg atatcctcac cgtcatagga gatggagccc gcatcgggtt cgatcagcct
     2401 caccagggtc ttgataaaga ccgtcttgcc ctggccgctg gggccgagaa tgaccatggt
     2461 ggaattatcc gggatatcga gatcgatgcc ggtgagcact tccttttcat cgaagctttt
     2521 gtggatgccg cgcacgctga tcatagcaaa agttttccca gataataatc aaaaatgacg
     2581 atgaaaaccg cggagacgac aacggcattg gtcgaggccg ctccgacccc cttggcgccg
     2641 ccgctggttt tgagaccgaa atagctgccc atcagggcga tgagcacgcc gtagacagcg
     2701 ggcttgatca ggttgccaaa gtacatctcg aagaccttga aatccatctc caggccgctg
     2761 ccgaactccg ccgccgagat ccattccgaa gtgaccgcgg agacgaaaaa ggagctgaag
     2821 atggcgagaa aattggcgaa gatggtgatc accggcagca tgagcagccc ggcgaccacg
     2881 cgcggcacga ccagaaagcc gaccggatcg agcgacatgg tctccagagc ctcaatctgt
     2941 tcggagatct tcatgctgcc gatctcggcc gccagcgagg ccccgagctt gccggccagc
     3001 accagggaga ggacgatcgg gcagagctcg agaatggtgg tcttgaagat gacattgacc
     3061 agcactgcgc gtgtaagcag ctcggaggac tgataggtgc tctgcacggt ggctccgagt
     3121 ccgatgaaga tggcgatcac cgccgcaatc ggcagggtct tgaccgaaaa ggcatagagc
     3181 tgctcgatga actggcggcg gtagaaccag agcgagggca gggcccggat tgtctgcatg
     3241 aagaggatgg cgatctcgta caccgtggtg aaaaaggaga tcgaacgctc gcccagcttt
     3301 tcaaaaaagt gttccgaagg tattcgtgcc attttacagg accattattt ttgacatgct
     3361 tcacagaggt aggagctgcg tccctcgtgg cgcaggcgaa cgatctggcc tccgcagcgc
     3421 gggcagggct cctcttcacg gccgtagacg gccagatcga gctggaaaaa tcccggctcg
     3481 ccgtcggcat tgcgataatc gctcagggtg gtgccgccgc gctccaccgc cttgccgagc
     3541 acacagatga tcgccagctg cagggcctcc ccctccgccg ggctcaggct ctgccccggg
     3601 cgcaggggat gaatccccgc ctgaaagagc gcctcgttgg cgtagatgtt gcccaccccg
     3661 acgacgacgc gttgatccat gatgactgtc ttgaccacac gtcgggtcgc ccccaggcgc
     3721 tcacggaaat actctgcagt aaaagccgac gagaggggtt caactcccag ttcgcacagc
     3781 atgggaagcc ccgccagttc ccccggcgcg gccagatcaa ggctgccgaa acggcgcgga
     3841 tcgcggaaat ggagctgacc gccttcctgg aaatggaaaa tgacgtgggt atggagttcg
     3901 gccagatgcg gcccatggtg ataacagagc cgtccgctca tgcccagatg gatgatgagg
     3961 gccccgccgc catcgagatg ccagatcagg tacttggcgc gccgctccag ggcggtgatg
     4021 cgctgccccg tcactgcacg cagttccgcc gccgtcggaa gcggccggcg cagtttgggc
     4081 gcatgaacct ccaccccggc aaggaccttc ccggtgatgt gcggggccaa actcctgcag
     4141 atcgtctcga cctccggcag ctccggcatc tcgtcactcc atcacgcccg aagagctctc
     4201 cggacgcatc atgattcccg ccctaaatgc tagtttagca aattctatgc taaaaaccaa
     4261 tcaataattt tgcacgatcg ggaaaaaaac cggctccagg gttatttcat ccggctctgg
     4321 aaacgcagca ctgcaaagct gaaaaccacc ccgccgaaga tcagcagggc cagcccctgc
     4381 gggtagagat ggcgcaccgt cgcactcttg atgaatatct cccgtatcac cagaacaaaa
     4441 tagcgcatcg gattgagcag actgatcccc tgcatcaccc gcggcatgtt ctcgatcgga
     4501 aagagaaaac cggacatgag catgaagaac atgagaaaaa agagggtcag aaacatcgcc
     4561 tgctgctggg tgcccgaggt ggccgagatg aagagaccca gccccagagt ggtgaagagg
     4621 tagaccaggg tgaagatgaa gagcagcccc aggttcccgg cgatgggaat gtcgtagatc
     4681 agcttggcaa cgcagagggc gaagaccacc tcgatcagcc cgagcaccgc aaaaggcacc
     4741 agcttgccga tgagcaactc atgtttgcgc agcggggcga ccagcagctg ctcgagggtg
     4801 ccgatctcgc gttcgcgcac cagtcccatc gccgcgacca gggtggtgac catggtgagg
     4861 agaaagacca caatccccgg caccatataa tcggaattct tcagctcctc gttgtaccag
     4921 acgcgcaggt tgggcgagat caggtggacc tctgcggccg cctcgctgcc ctgcagcttc
     4981 ccctcgatct gatccatgat ctcgctctgc aaaatgccgt tgatatagcc cagggcgatg
     5041 gtcgaggtgt tggaatcctg gccgtccatc agcacctgca gtcccgcggg ctccccccgc
     5101 tgcagccggg tgctgaaatc ggccggtatg accaccgcca ccaccgcccg gccgttgtcc
     5161 agatagtcgt ggagatggtt ctgccgggta tcgaaaaagg cgagatcgaa atagcctgag
     5221 ttgcggatgc gctcgacgag cagccggctg gtcgaggaat tgtcgagatc gcagatcacg
     5281 gtccggatgt tgtggacctc gctggagatg acaaaaccga gcaacagcag ctgcaggatc
     5341 ggggccacaa agaggatcat cagcatgccg cggtcgcggc gcacctggat gaactccttt
     5401 ttgatgatat gcaaaatttt ccccacagcc tcaaccctcc agattgattt tgaaacgctt
     5461 gacgctgatc aggatcagca gggtgccgaa gagcaccagg aagagggcgg gctgccagag
     5521 caaggggccg gcaatcccct tgagcatgat gctgcggatg atgaccagaa agtatttggc
     5581 cgggacgata tagctgagca gctggagcag cttcggcatc gaggccaggg gaaagataaa
     5641 tcctgagagc aggatcgcag gcagcagggt ggagaccatg gtcatcatca gggccatctg
     5701 ctggttgggg acccgcgccg agacaaagat gccgaagctg aggcaggtgt agatgaagat
     5761 cagggcgtag ccgagcagcc agaggggttc gccgcggaac gggacgccga accagagcca
     5821 ggcaaaaacc accaccacaa tcccgtcgag cagcccgagc aggatatagg gcagggtctt
     5881 gccggcgatg atctccagcg agcgcaccgg cgagaccagg atctgctcca tggtgccggt
     5941 ctcgcgctcc cgcgccaccg tcaccgaggt gagcagggcg cagatcaaca tcagtatcac
     6001 cgccaccagg ccggggacga taaagttgac gctcttgaga tcggggttgt accagac
//
LOCUS       DAOPWU010000202         6044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13002_length_6044_cov_4.039740, whole genome shotgun sequence.
ACCESSION   DAOPWU010000202 DAOPWU010000000
VERSION     DAOPWU010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13002_length_6044_cov_4.039740"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2155
                     /locus_tag="PKM23_10290"
     CDS             <1..2155
                     /locus_tag="PKM23_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91903.1"
                     /translation="IGYRPAFKMVQVGQEISELRFELTTSPLPVMDMTITMPRYATTL
                     SESALPLSVVSSEKLESTAPTTVSDALQSEPGIALQRDGIWATSVSIRGLGRNSIVTL
                     IDGNRIDTATDLASGLSMFDMQEIERIEVIKGAASALYGSGAMGGVINVISRDGWYQE
                     TPYWHARLMSRYASVNNGGQGHITLNAGGSKWYGQFSGALRKADDTETPTGMLENSQY
                     RDNNLSARLSLRPLQNHELKFNYQRFYAEDVGMPGGYPVFSSSATVRYPFEKRELMSG
                     EYIASHISRHLQQLSVKLYNQKIWRDVENIPHTVTSIPASGTQPARKVNALRILPGAT
                     HDLNGLQVQGDFFPGKHHLLIAGLDAWQKAYEGYRTKETRIDILNPDGSVKKSTFRTI
                     GEAPLPDATYSSIGLYAQDEWRLLEERLILGLGGRIDQIKTENDQTLNPLYEIVDGVR
                     NNAPAGQIVLWPEESATDHSWSGNFSLLYRIREQMDLTLNLGRSFRVPSLEERYQYID
                     QGSLVKVGDPDLEPEQGGFTDLGLRIWSDRLQLQVNGFYNRMNNLVAEMPGSYENRKA
                     LLKTNIGRAELYGCDARIDFKPAQGCAIWARAAYVHGEDLRTNKPLSQIAPLNGSIGY
                     HNGCLNWLSIDLSAMMFATQERLADGEVRTPGYVYYNFYLNSRAIPLGSARGFLTVGV
                     ENITNRAFRNHLSTNRGLVAIEPGRNFTATWRMEI"
     gene            complement(2188..2391)
                     /locus_tag="PKM23_10295"
     CDS             complement(2188..2391)
                     /locus_tag="PKM23_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNY91904.1"
                     /translation="MNRSLRLALGALIGAAGGFAFYYFIGCRTGSCPITGNPFISSGY
                     GALMGVLLLFPSSPKKRASTGSE"
     gene            complement(2443..2652)
                     /locus_tag="PKM23_10300"
     CDS             complement(2443..2652)
                     /locus_tag="PKM23_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNY91905.1"
                     /translation="MPIYEYRCSDCGHRFEVFQRMGADGSDLQCPLCAAPKPEKLFSA
                     IAASSKSGSSGCASPSPGCGSGGFS"
     gene            complement(2889..3797)
                     /locus_tag="PKM23_10305"
     CDS             complement(2889..3797)
                     /locus_tag="PKM23_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNY91906.1"
                     /translation="MKGSATTPPSLLIGPAGWHYEDWKGIVYPRRPARAQHELAFLAD
                     FLDLVEVNSSFYRIPDPGSAHNWLKQSAHNPRFRFIIKLWQGFTHEPEPAIGGEMTAF
                     KRLLEPLLEKERLLTVLVQFPWSFKKSEQHLAQLERIVCALRPYPCHVEFRHNSWQDP
                     ALILLLRREKIGWVNIDQPVIGDSMGPASLATTHIAYYRFHGRNYQNWFREGAGRNQR
                     YDYLYNSDELGEWLLRIKETLPETEKTAVVFNNHFKGQAVVNSLQLLALLTEAKPQVP
                     PELAEHYPELGGFVTLPPMQGTLPLF"
     gene            complement(3937..4716)
                     /locus_tag="PKM23_10310"
     CDS             complement(3937..4716)
                     /locus_tag="PKM23_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNY91907.1"
                     /translation="MTLQPITIVIILVVLFLLSAIKVLREYERAVVFRLGRLVGSRGP
                     GIFILIPLVDRMIKVSLRTIAMDIPTQDVITKDNVSVKVNAVLYFRVMDPAKAVVQVE
                     DYLYATSQIAQTTLRSILGQAELDDLLAEREKLNRELQTIIDAQTEPWGIKVTLVEVK
                     HVDLPQEMQRAMAKQAEAERERRAKVIHANGEYQASKTLAKAAEIMAEHPQSLQLRYL
                     QTLSEIATENNSTIVFPLPIDLIKPMLETIKMVPKPEQGKK"
     gene            complement(4749..6038)
                     /locus_tag="PKM23_10315"
     CDS             complement(4749..6038)
                     /locus_tag="PKM23_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNY91908.1"
                     /translation="MLIAAIAGLAPAAAEEAEVLKITIDGDINPVSAMYIVKAIERAE
                     KEEYTCVIIQMDTPGGLLESTKSIVKEILTARVPVVMFVAPSGSGAVSAGVFITLACH
                     VAVMAEGTNIGAAHPVSAGGGADTSRVMTEKVTNYASAWIRSIAEKRGRNPEWAEMAV
                     RKSVSITEKEALEKNIIDLVVKDEAELLQALDGREVQTDDGSRVIKTRNAQVISLPMT
                     WQHRILYKISNPTIAYILLMLGVYGLFFELSNPGSIVPGVAGGIFIILAFFALQTLPL
                     NTAGLLLILFAILLFILEVKVTSYGILTIGGIVSMFLGAIMLFDEAPGFPVRVDWRVA
                     LTFALATAAFFVFALGLALKARLSRPTTGQEGLIGAHGVALTRLSNEEGEVKVQGEIW
                     QARSDQEVAKGASIEVVHVDGLVLTVRPQHSQKESSI"
BASE COUNT         1311 a         1742 c         1776 g         1215 t
ORIGIN      
        1 tatcggctat cgtcctgcct tcaagatggt gcaggttggg caggaaatca gcgagctgcg
       61 ttttgaactt accacctccc ccctgccggt gatggatatg accatcacca tgccgcgtta
      121 tgctaccacc ctgagcgaat ccgccctgcc cttgagtgtg gtgagcagcg aaaagctgga
      181 gagcacggcg ccgaccaccg tttccgacgc cctgcagagc gagccgggga tcgccctgca
      241 gcgggacggc atctgggcca ccagcgtcag catccgcggg ctcggccgca acagcatcgt
      301 caccctgatc gacggcaatc gcattgatac ggcgacggat ctcgcctccg ggctctccat
      361 gttcgacatg caggaaatcg aacgcatcga ggtgatcaag ggggctgcat cggctctcta
      421 cggttccggc gccatggggg gtgtgatcaa tgtcatcagc cgggatggct ggtaccagga
      481 gaccccttac tggcatgcgc gcctgatgag cagatacgcc agcgtcaaca acggtggtca
      541 gggtcacatc accctcaatg ccggcggcag caagtggtac ggtcagttca gcggcgcgct
      601 gcgcaaggca gacgacaccg aaacccccac tggcatgctg gaaaacagcc agtatcgcga
      661 taacaacctc tcggcgcggc tctccctgcg gcccttgcag aaccatgagc tcaagttcaa
      721 ctaccagcgc ttttatgcgg aggatgtggg catgccgggc ggatatccgg tcttctcgag
      781 cagcgctacg gtgcgttatc cctttgaaaa gcgcgagctc atgagcggcg aatatatcgc
      841 cagccatatt tcgcgccatc tccagcagct ttccgtcaag ctctacaatc agaaaatctg
      901 gcgcgacgtc gagaacatcc cgcacaccgt cacttcgatc cccgccagcg gcacgcagcc
      961 ggcgcgcaag gtcaacgccc tgcgtatact tcccggcgcc acccatgatc tgaacggcct
     1021 gcaggtgcag ggggattttt tcccgggcaa acaccacctt ctcatcgccg gcctcgacgc
     1081 ctggcaaaaa gcctatgaag gttatcgcac caaggagacc cggattgaca ttctcaatcc
     1141 ggatggctcg gtcaaaaaga gcaccttccg cacgatcggc gaagcccccc ttccggatgc
     1201 cacctacagc agcatcggcc tctatgccca ggatgaatgg cggctgctgg aggaacggtt
     1261 gatcctggga ctgggcggac gcatcgacca gatcaaaacc gagaacgatc agacccttaa
     1321 ccccctctat gaaatcgtcg acggagtgcg taacaatgcg ccagccggtc agatagtgct
     1381 ctggccggag gaatccgcca ccgaccattc atggagcggc aatttttcac tgctctatcg
     1441 catcagggaa caaatggatt tgaccctgaa tctgggccgc tccttccgcg tcccttcact
     1501 cgaagagcgc tatcagtaca ttgatcaggg cagcctggtc aaggtgggcg atcctgatct
     1561 cgagcccgag caagggggtt tcaccgacct cggcctgcgc atctggtccg accggctgca
     1621 gctgcaggtg aacggctttt ataatcgcat gaacaatctg gtagcggaga tgcccggaag
     1681 ttacgagaac cgcaaggcct tgctcaagac caacatcggc agggcggaac tgtacggttg
     1741 cgacgcgcgg atcgatttca agcccgcgca gggatgcgcc atctgggccc gtgcggccta
     1801 tgtgcacggt gaggatctgc gaacaaataa acccctgtca cagatcgccc cgctcaatgg
     1861 cagcatcggc taccacaacg gctgcctcaa ctggctgtcg atcgatctct ctgccatgat
     1921 gttcgcaacc caggaacgtc tggcggacgg cgaggtgcgg acacccggat atgtctacta
     1981 caacttttat ctcaacagcc gtgcgatacc gctgggcagc gccaggggct tcctgaccgt
     2041 cggtgtcgag aacatcacca accgcgcctt caggaaccat ctgtcgacta atcgcggact
     2101 ggtcgccatc gagccggggc gcaacttcac cgccacctgg cggatggaga tttaacaaaa
     2161 agggctgctt ttgagcagcc cttttcttta ttcactaccg gtggatgccc gctttttggg
     2221 gctggaggga aaaaggagga gcacccccat caacgcgcca tagccgctgc tgatgaaggg
     2281 attgccggtg atggggcagc tgccggtgcg gcagccgata aaataataaa atgcgaaacc
     2341 gccagcggcg ccgatcaggg caccgagggc gagacgaaga gatctgttca tattctatcc
     2401 ttacagtttt ggatgcggcc caactgaccg cgatcaggat gctcaggaaa aaccgccgct
     2461 gccgcacccg ggcgaaggag aagcacagcc ggatgagccg cttttggagg aggcggcgat
     2521 agccgaaaag agcttttcgg gttttggcgc agcacagagc gggcactgca gatccgaacc
     2581 atcagcgccc atgcgctgaa agacctcaaa gcgatgaccg caatcgctgc atctatactc
     2641 gtagattggc atctaaacta ttccttttat gcactttccc tctcctcatc gcctctatcc
     2701 cttttctctt cctccggaag agcacaagag gctgacattc aggtcggaac gcccagccgc
     2761 ggcaggacga accggaccag gatgaaccag accgcaaaca aagcaagtat gcccagactg
     2821 tccatggcgt ctccttatgt tacagacaag aattggatgc agaatgagga aaaagggttc
     2881 acgcgatttt aaaagagggg cagggtgccc tgcatgggcg ggagcgtgac aaaaccaccg
     2941 agctcgggat aatgctccgc aagctcaggg ggaacttgcg gtttggcctc tgtgagcagg
     3001 gccaacagct gcaggctgtt gactaccgcc tggcccttaa agtgattatt gaagacaacg
     3061 gcggtttttt cggtttcagg aagggtttcc ttgattctga gcagccattc gcccagctcg
     3121 tcagagttat agagatagtc gtagcgctga ttacgacctg ctccctccct gaaccagttc
     3181 tggtaattgc ggccatgaaa gcggtaatag gcgatatgag ttgtggccag gctggccggt
     3241 cccatcgagt cgccgatgac aggctggtcg atgttcaccc agccgatctt ttcccgccgc
     3301 agcagaagaa taagtgcggg atcctgccag gagttgtgac ggaattcgac atgacaggga
     3361 tagggacgga gagcgcagac gattcgttcg agctgtgcga ggtgctgctc cgatttttta
     3421 aaactccagg ggaactggac cagcacagtc aacagcctct ccttctcaag gaggggttca
     3481 agcagcctct tgaatgcggt catctccccc ccgatggccg gttccggctc gtgggtgaat
     3541 ccctgccaga gcttgataat gaaccggaat cgggggttat gagcgctctg cttgagccag
     3601 ttgtgcgcag agcccggatc gggtatgcga taaaagctgc tgttgacctc gaccagatcg
     3661 agaaagtcgg cgaggaaagc gagctcatgc tgggcccggg cggggcgtcg gggatagacg
     3721 attcccttcc aatcctcgta atgccagccc gccggcccga tgagaagaga tgggggtgtc
     3781 gtggcgctgc ctttcatgct gatttgaaaa aacaggggcg gtctgcggac cgcccctgtt
     3841 gccttcactt atgctggaaa cggcgctcag ccgcgtcaca gttcgagccg gtgtgcatct
     3901 gccacagtca gatgcacgac agctgtggtt tgcggattac ttcttgcctt gctccggttt
     3961 gggtaccatc ttgatcgttt caagcattgg cttgatcagg tcaatcggca gggggaagac
     4021 gatggtggag ttgttctccg ttgcgatttc gctcaaggtc tgcagataac gcagctgcag
     4081 ggactgggga tgttcagcca tgatctccgc cgccttggcc agggtcttgg aggcctgata
     4141 ttcgccgttg gcgtggatga ccttggcacg gcgttcgcgc tcggcctccg cctgtttggc
     4201 cattgctctc tgcatctcct ggggcaagtc gacatgcttg acctcgacga gagtgacctt
     4261 gatgccccag ggctcggtct gggcatcgat gatggtctgc agttcgcgat tcagtttttc
     4321 gcgctcggcg agaagatcgt ccaactcggc ctgaccaagg atcgaacgca gggtggtctg
     4381 ggcgatctgc gaggtggcat agagataatc ctccacctgt accaccgcct ttgcgggatc
     4441 cataacgcgg aaatagagca cggcattgac cttgacggaa acattatcct tggtgatgac
     4501 atcctgggtc gggatatcca tggcgatggt gcgcagactg accttgatca tccggtccac
     4561 cagggggatg aggatgaaga tgccggggcc gcgcgatccc accaggcggc ctaagcggaa
     4621 aaccaccgcg cgctcatatt cacgcaatac tttaatcgca ctcagcaaaa acagcacaac
     4681 aaggataatt acaatcgtaa tgggctgcag agtcatagga cctccttgca cgtaatggga
     4741 tgagttgctt aaatggacga ttccttttgc gagtgctgag gacggacagt cagcaccaga
     4801 ccatcgacat ggaccacttc gatcgatgct cctttggcca cctcctgatc ggagcgcgcc
     4861 tgccagattt ccccctgcac cttgacctcg ccctcctcat tggagaggcg ggtgagagcg
     4921 acgccgtggg cgccgatgag cccctcctga ccggtggtcg gccggctcaa gcgcgccttc
     4981 agggccaggc cgagggcgaa gacgaaaaag gcggcggttg ccagggcgaa ggtaagggca
     5041 acccgccagt ccacgcgcac ggggaagccc ggggcctcgt cgaaaagcat gatcgctccg
     5101 aggaacatgg agacaattcc accaatggtg agaattccgt agcttgtcac cttgacctcc
     5161 agaatgaaga gtagaatggc gaagaggatg agcaacagtc cggctgtatt caagggcagg
     5221 gtctgcaggg cgaagaaggc cagaataata aaaatgccgc cggctacgcc cggaacgatc
     5281 gagcccggat tggagagttc aaagaagaga ccgtagacgc cgagcatgag caggatgtag
     5341 gcaatggttg gattggagat tttgtataag atcctgtgct gccaggtcat tggtagtgaa
     5401 atgacctggg cattacgcgt cttgatcacc cggctgccat cgtcggtctg cacctcacgg
     5461 ccgtcgaggg cctgcaggag ttcagcctca tccttgacga ccagatcgat gatatttttc
     5521 tccagtgcct ccttctccgt gatcgagacg ctcttgcgca ccgccatttc ggcccactcc
     5581 ggatttctcc cccgcttctc ggcgatgctg cgaatccagg ctgaggcgta gttggtgacc
     5641 ttctcagtca tcaccctgga ggtatcggcg cctcccccgg cgctgaccgg gtgggcggcg
     5701 ccgatattgg tcccctcggc cataaccgcg acatgacagg ccagggtgat aaagaccccg
     5761 gcagagacgg cccctgatcc gctcggagcg acgaacatca ccaccggcac cctggcggtg
     5821 agaatctcct tgacgatcga cttggtcgat tcgaggagtc cacctggtgt gtccatctgg
     5881 atgatcacac aggtgtactc ctctttttcg gccctttcga tggccttgac tatatacatg
     5941 gcggagacgg gattgatatc gccatcgatg gtgattttga ggacctccgc ctcttctgca
     6001 gcggcgggcg caaggccggc aatcgcagcg atgagcatga aggc
//
LOCUS       DAOPWU010000203         6037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13029_length_6037_cov_5.452357, whole genome shotgun sequence.
ACCESSION   DAOPWU010000203 DAOPWU010000000
VERSION     DAOPWU010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13029_length_6037_cov_5.452357"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2349
                     /locus_tag="PKM23_10320"
     CDS             <1..2349
                     /locus_tag="PKM23_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HNY91909.1"
                     /translation="IEGFWDGDDIWKVRMAPTETGQWSYQISGSNAAFNRSGSFTCTA
                     SSSHGFIRPNPARPYSFMHDDGTPWLWKGDTNWRGFTSLLPYDGRWKEIVDLRAEQGY
                     TAMQSIVVSYINGLGFWKNEGGTCFVEGTDAKDYDQLNPGYFRWIDKRIDYALSKGIV
                     PVILFSWAQEYVNFSPEQWERYVRYLVARLSSRNVIWVLCGEYNEIPNDYNRPTSEFA
                     DWGQMVKRYDPYDHPITLHPTGRTSSAEFGSSAWMDVVMQQTPYFSRDIQRDRVHKKP
                     VVNGEPRYMYPDEDNAESRHGLWEIVTSGGYYTGGFFTTYAPDKGGYDPAAMPEEQRW
                     VTFLNKLMDRLPISEMEPHPEWTSAGQLLAKPGAEYLAYNRSGGPVTIDLSKVSGSLP
                     VEWLDPRAGVSQPVGKVSGGAKQTFTPPFSGDWALHIGAGLSSDEVAPNPPQALQSTA
                     RTMYSITLSWSAPAAAADGDVAASYIITRDGTAITTVTATTWTDQGLEESSSHTYAIH
                     ALDDAGNKSSSAAQLTVSTTGDTQAPQVTQVITVSGTELRVTFDEKVEAASAQTPGNY
                     VITPAVAVTAALLSAEGTQATLTTAAHNPGQSYTIKVAGIKDRARVANTMAAQTLNYK
                     FEATLQISELSPATYRLAQLAAGSAYYADRDFKLTTIPAVCQGFTWIMTENDDKERSD
                     AHWLSFKANMAVTVMIAYDNGASTRPTWLDGWSDTGAEIGTSDDSPLHLYRRDFPAGE
                     VVLGGNEGTSKTSMYLVLVKKADGTVADTTPPAVPKGLDFAG"
     gene            2390..2608
                     /locus_tag="PKM23_10325"
     CDS             2390..2608
                     /locus_tag="PKM23_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91910.1"
                     /translation="MDYTYDQLAGKEDQELFDFQLQFPPNHHNFLLAEQILEERSRRE
                     LRSVETRSLVIAIAALTIAFILFAMSVL"
     gene            3086..3640
                     /locus_tag="PKM23_10330"
     CDS             3086..3640
                     /locus_tag="PKM23_10330"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNY91911.1"
                     /translation="MPTPIGHIASGLLLFAAGSRSWRKQAGVLLALFFFALLPDIDLA
                     FGLLLAGNANAFHHQATHSIFFVVAAGWIGGQLLGQKGRHAPLLITAGLIHLALDLLA
                     KDTSAPYGAPVLWPFWNGYIIAPVRVFSDVQRSGDAASFLPSLFNRHNLQTIGIEMAV
                     FGLLWGLWSAWSYSRRGYERKASS"
     gene            3618..4577
                     /locus_tag="PKM23_10335"
     CDS             3618..4577
                     /locus_tag="PKM23_10335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015227211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY91912.1"
                     /translation="MNEKLRAEGSETPFLSVVVPIYNEVESVERLWAAIHPVMEGLGR
                     PYEVILVDDGSRDGTREILRRLASANPRLRVILFRANFGQSAAMAAGFAATRGEIVVA
                     MDGDLQNDPLDIPQLLEKMHEGYDVVSGWRKNRKDKLVLRKVPSKIANKLICSLTDVR
                     LHDTGCSLKAFRGEMLRRISLYGELHRFIPALMRIEGAEITELAVRHHPRRFGTSKYN
                     LSRTFRVVMDLTSIRLLMKHLVNPLVFFSAVALFFALLGSLSLAAILHAAAARGAELA
                     EMNILVTLTVVFWAAAAQLFFFGLIGKLIVQTGTRKTFLQTEQ"
     gene            4655..5614
                     /locus_tag="PKM23_10340"
     CDS             4655..5614
                     /locus_tag="PKM23_10340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY91913.1"
                     /translation="MQQPLTPVRAAASFSVIVLTYNNAGEIAGMHREIMAALAEWRQP
                     HELIYVDDGSTDTTWKELLRISRSETGVKAARLRSTFGEASALDAGVQIATGEVLLYF
                     TCRVRIKPADALALLKQIDAGEDVVIGVRYPRRDSGLNRIVSRTFNGLTNRMTGLHLH
                     DVNSGVLALRREVLDHVPYYGTLNAFLPVLAQRQGYRIAEGKVEQLPGRFNQSVYPKN
                     YIRRSLDLVSVIFLSKYSKKPLHFLGFIGALFALIGAGINIYLFIYRILGFGGIAGKP
                     MLLLGSILFIIGIQMVSIGLLAEMIIFTHAKEIKDYNIEEIIN"
     gene            5618..>6037
                     /locus_tag="PKM23_10345"
     CDS             5618..>6037
                     /locus_tag="PKM23_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY91914.1"
                     /translation="MKLIIQIPCFNEAETLPLTLADLPRHIAGVDEIEVLIIDDGSSD
                     ETTEVARRSGVHHIVRMTKNKGLANGFMAGLDASLRLGADIIVNTDADNQYNGADIPS
                     LVRPIIDGQAELVIGDRQTDEIHHFSWMKKRLQNLGSW"
BASE COUNT         1170 a         1953 c         1778 g         1136 t
ORIGIN      
        1 atcgagggtt tttgggacgg tgacgatatc tggaaggtgc gcatggcccc caccgaaacc
       61 gggcagtgga gctatcagat cagcggttcg aatgccgcct tcaaccgcag cggcagtttt
      121 acctgcaccg ccagtagctc gcacggtttt atccgcccca atccggcgcg gccctactct
      181 ttcatgcacg acgacggcac cccctggctc tggaagggcg ataccaactg gcgcggcttc
      241 accagcctgc tgccctacga cggacgctgg aaagagatcg tcgacctgcg cgccgagcag
      301 ggctacaccg ccatgcagtc gattgtggtc agctatatca acggcctcgg tttctggaag
      361 aatgaagggg gcacctgttt cgtcgagggg actgacgcca aggactacga tcagctcaat
      421 cccggctatt tccgctggat cgacaagcgc atcgattatg ccctgagcaa ggggatcgtt
      481 ccggtcatcc tcttcagctg ggcccaggaa tatgtcaatt tcagcccgga gcagtgggag
      541 agatatgttc gctacctggt cgcccgcctc tcctcgcgga atgtgatctg ggtgctctgc
      601 ggcgagtaca acgaaattcc caacgactat aaccgcccga ccagcgagtt tgcggattgg
      661 ggacagatgg tcaaaaggta tgatccctat gatcatccca tcaccctgca tccgaccggc
      721 cgcaccagca gcgctgagtt tggctcgtcg gcctggatgg atgtggtgat gcagcagacc
      781 ccctattttt cgcgcgacat tcagcgcgac cgggtgcaca aaaagccggt ggtcaacggc
      841 gaaccgcgct acatgtatcc ggacgaggac aacgccgaga gccgccacgg cctctgggaa
      901 atcgtcacct cgggcggcta ctacaccggc ggttttttca ccacctatgc tccggacaag
      961 gggggctacg atcccgccgc catgccggag gagcagcgct gggtcacctt cctcaacaag
     1021 ctgatggacc gtctgccgat ctccgagatg gagccccatc ccgagtggac cagcgccggt
     1081 cagctgctgg ccaagccggg tgccgagtat ctggcctaca accgcagcgg cggtccggtg
     1141 acgatcgacc tgagcaaggt cagcggctcg ctgccggtcg aatggctcga tccccgcgcc
     1201 ggggtgagcc agcccgtcgg caaggtgagc gggggagcga aacagacttt tacacccccc
     1261 ttcagcgggg attgggctct ccacatcggc gccggcctga gctccgatga ggtcgcgccg
     1321 aatcctccgc aagccctgca aagcaccgcg cggaccatgt acagcatcac cctctcctgg
     1381 agtgctccgg cagcggccgc cgacggggat gtcgccgcca gctatatcat cacccgcgac
     1441 ggtacggcga taaccaccgt cacagcgacg acctggaccg accaggggct ggaagagtcc
     1501 tcctcccaca cctatgcgat ccatgccctg gatgacgccg gcaacaagag cagctcggcg
     1561 gcccaactca cggtttccac gaccggggac acccaggcgc ctcaagtcac gcaggtgatt
     1621 accgtctcgg gcacggagct ccgcgtcacc tttgacgaaa aggtggaggc cgcatccgcc
     1681 cagaccccgg gcaactatgt catcacaccg gctgtggccg tcaccgcggc gctgctctcg
     1741 gccgagggga cgcaggcgac cctgaccacg gcggcccaca atcccggcca gagctacacc
     1801 atcaaggtcg ccgggatcaa ggaccgcgcc cgcgtcgcca ataccatggc ggcgcagacg
     1861 ctcaactaca agttcgaggc gaccctgcag atcagcgaac tcagtccggc gacctaccgg
     1921 ctggcccagc tcgccgccgg cagcgcctat tatgccgacc gcgatttcaa gctcactacc
     1981 atcccggccg tctgtcaggg tttcacctgg atcatgaccg aaaacgacga caaggagcgc
     2041 agcgacgccc actggctctc cttcaaggcc aatatggcgg tgacagtgat gatcgcctac
     2101 gacaacggcg ccagcacccg gccgacctgg ctggacggct ggagcgacac cggcgcggag
     2161 atcggcacca gtgatgattc gccgctgcat ctctatcgcc gcgatttccc ggcgggggag
     2221 gtggtgctgg gcggcaacga agggaccagc aagacctcaa tgtatctggt tctggtgaaa
     2281 aaggcggatg gcacggtcgc ggataccacg ccgcccgctg tacccaaagg tttggatttt
     2341 gcggggtagg cgccgggggc gccgctcgca gaaagaaaca ggcaggaaga tggactatac
     2401 ctatgaccaa ttagccggca aggaggatca ggagctcttt gactttcagc tccagtttcc
     2461 ccccaaccat cacaatttcc ttctcgcgga gcagatcctc gaggagcgca gccgcaggga
     2521 gctgcggtcg gtcgagacca gatccctggt tatcgccatc gcagcgctga ctattgcctt
     2581 tattctgttc gctatgtctg tgctctgagt acgcagtcaa cggggaattc tcgcatttgc
     2641 cgccggcggc tgctcatttg acagccgccg gtgcatgacc atgcagagcc ggttacaggc
     2701 ggctgttcgg acctccccgg gccaaaaacg caaaaaacag gtttgtaaat aatttattat
     2761 ttactatatt tcctcgggat gcaccgggat gcggcccgcg gaatggcact cctttgccct
     2821 ggtttttctc tctctcgggt cttgccaggg cctttttata tcccggcctg aagagcgccg
     2881 gcacaccctg aaatctttcg gcaaaagcgc cctcttgttg ctacctaata gtgcgcggaa
     2941 aggcgcgcag ccgagctgga gaaagacaca tcgctgccgg ctgtgcgcgg caaggtgcac
     3001 ggccgggatc aggaaaatgc ccgcagcaaa cggctttcaa caagggaacg gatcgggcgg
     3061 gttggatggg acgacggaaa acgagatgcc gacaccaata ggtcatatag ccagcggcct
     3121 cctgctcttc gctgccggca gtcggtcctg gcggaagcag gccggcgtgc tcctggccct
     3181 gttctttttc gccctgctgc ccgacatcga cctggccttc ggcctgcttc tggccggcaa
     3241 cgccaacgcc tttcaccatc aggccaccca ttccattttt ttcgtcgtgg ccgccggctg
     3301 gatcggcggc cagctgttgg ggcaaaaagg gcgccatgca ccgctcctga tcaccgccgg
     3361 cctgatccat ctggccctgg acctcctcgc caaggatacc agcgccccgt acggcgcccc
     3421 ggtgctgtgg cctttctgga acggctacat catcgctccg gttcgggtct tctccgatgt
     3481 gcaacgcagc ggcgatgcgg cctccttttt accgagcctg ttcaacaggc acaatctgca
     3541 gacgatcggc attgagatgg cggttttcgg gctcctttgg ggcctgtggt ccgcctggag
     3601 ttattcaaga aggggatatg aacgaaaagc ttcgagctga ggggagcgaa acgccctttc
     3661 tctccgtcgt cgtccccatc tacaacgagg tggagagcgt cgagcggctc tgggcggcta
     3721 tccatccggt gatggagggg ctggggcgtc catatgaggt gatcctggtc gatgacggca
     3781 gccgcgacgg cacccgcgag atactgcgcc ggctggcatc ggccaatccc cggctgcggg
     3841 tgatcctctt ccgcgccaat ttcggccagt ccgcggccat ggccgccggc tttgccgcca
     3901 cacgcggcga gatcgtcgtg gccatggacg gcgatctcca gaacgacccc ctcgacatcc
     3961 cgcagctgct cgaaaaaatg catgagggct atgatgtggt gagcggctgg cgcaagaacc
     4021 gcaaggacaa gctggtgctg cgcaaggtgc cgtcaaaaat cgccaacaag ctgatctgct
     4081 ctctcaccga cgtccggctg cacgataccg gctgttcgct caaggccttt cgcggcgaga
     4141 tgctccgccg catctctctt tatggggaac tgcaccgttt catcccggcc ctgatgcgca
     4201 tcgagggcgc cgagatcacc gaactggcgg tgcgccacca cccgcgccgt ttcggcacct
     4261 cgaaatacaa tctcagccgc accttccggg tggtgatgga tctgaccagc atccggctgc
     4321 tgatgaagca cctggtcaat cccctggtct ttttttcggc ggtcgccctc tttttcgccc
     4381 tgctcggttc tctcagcctc gccgccatcc tgcatgcggc agcggcgagg ggcgccgagc
     4441 tggcggaaat gaatatcctg gtcaccctga ccgtggtttt ctgggcggcg gcggcacagc
     4501 tttttttctt cggtctgatc ggcaaattga tcgtccagac cggcacacgc aagacctttc
     4561 tgcagacgga acagtaaacc ggccccctgc gggcaggcgt gaagattttt aaccgttatt
     4621 ctatcagcgg cctgatatga cagaatccca aaacttgcaa cagcccctca cccctgtacg
     4681 ggcagcggcc tccttttcgg tcatcgtcct gacctacaac aatgccgggg agatcgccgg
     4741 catgcaccgt gagatcatgg ctgcgttggc ggagtggcgg cagcctcatg aactcatcta
     4801 tgtggatgac ggcagcaccg acaccacctg gaaggagctg ctccgcatca gccgcagcga
     4861 gaccggggtc aaggcggcac ggctgcgctc gacctttggc gaggcctcgg cgctcgacgc
     4921 cggggtgcag atcgccacag gagaggtcct gctctacttc acctgccggg tgcgcatcaa
     4981 acccgccgat gccctcgccc tgctgaagca gatcgatgcc ggggaggatg tcgttatcgg
     5041 ggtgcgctac ccccgccgtg actcggggct gaaccggatc gtctcgcgca cttttaacgg
     5101 tctgaccaac cgcatgaccg gcctccacct ccacgacgtc aacagcgggg tcctggccct
     5161 gcggcgcgag gtgctcgacc acgtccccta ctacggcacc ctcaatgcct ttttgcccgt
     5221 gctggcgcag cgccagggct accgcatcgc cgagggcaag gtcgaacagc tgccgggccg
     5281 tttcaaccag tcggtctatc cgaaaaacta catccgccgc agcctcgatc tggtcagcgt
     5341 catttttctg agcaaatact cgaaaaagcc cctccacttc ctcggcttca tcggcgcgct
     5401 tttcgccctc atcggtgcag gcatcaacat ctatctgttt atctaccgga tcctcggctt
     5461 cggcggcatc gccggcaagc cgatgctgct gctcgggtcg atcctcttca tcatcggcat
     5521 ccagatggtc tccatcggcc tgctggcgga gatgatcatc ttcacccacg ccaaggagat
     5581 caaggattac aatatcgagg agatcatcaa ctagaccgtg aaactgatca ttcaaatacc
     5641 ctgcttcaat gaggcggaga ccctgcccct gacccttgcc gatctgccgc gccatatcgc
     5701 cggcgtcgat gagatcgagg tgctgatcat cgacgatggc agcagcgacg agaccacgga
     5761 ggtggcgcgg agatcgggcg tccaccatat cgtgcgcatg accaagaaca aggggctggc
     5821 caacggtttc atggccgggc tcgacgcttc gctgcgtctc ggcgccgaca tcatcgtcaa
     5881 caccgacgcc gacaaccagt acaatggcgc ggacattccg agcctggtgc gccccatcat
     5941 cgacggccag gccgagctgg tgatcggcga ccgccagacc gacgagatcc accatttttc
     6001 gtggatgaaa aagcggctgc agaacctcgg cagctgg
//
LOCUS       DAOPWU010000204         6031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13056_length_6031_cov_4.473728, whole genome shotgun sequence.
ACCESSION   DAOPWU010000204 DAOPWU010000000
VERSION     DAOPWU010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13056_length_6031_cov_4.473728"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1716
                     /locus_tag="PKM23_10350"
     CDS             178..1716
                     /locus_tag="PKM23_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNY91915.1"
                     /translation="MPTLNAHLEKVANRVPENPAMVFGSQRVDYRRFHQAVRRLAGAL
                     QALGIGRGDRVALHLPNLPHFCISYYALLHLGAVVVPLSILSKESELAAILQHSGCRG
                     IISWSGMLAPLQGALEQARECTLQLVLGEQIPRHAHSLTRLIAQSPELTSAPAAGPDD
                     LAVINYIAGGAEENLGAEFTQAALVAGATTSSEMFRITVDDRVLAVLPLFHPLGQSLV
                     MHGAFYAGATVVLQPRFQASEVVTALREHQVTFMAGVPGMFKLLAQMDPPDPPLSTLR
                     YALSYGGQLQEGVLETFEEKFHAHLLESYGFTEAGPLVTSMRINRDRKPGSVGLPMVG
                     VELQILDEEGRLLRPNQSGEIWVKSPSLMQEYYRKPDRTQQRLRDDWFFSGDVGYLDD
                     EHYLFVQERKEDIITKGGFQIFSFEVEEVLLGHPAVAEAAVVGVPDPAQGQEVKACVV
                     LKAGEQVSRDELISWCRSSLPVYKSPKIIEFVPVLPKSPTGRVLKNILRAEARSPASA
                     HGKQ"
     gene            1779..3866
                     /gene="fusA"
                     /locus_tag="PKM23_10355"
     CDS             1779..3866
                     /gene="fusA"
                     /locus_tag="PKM23_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNY91916.1"
                     /translation="MKDYGPNDIRNVGLVSHQSTGKSTLVEGMLFAAGAITRLGTIDD
                     GSTTSDYRPDEIDRKISISAALMTIDWKKNKFNLLDLPGYPDFIGEVHCALRVTDLTI
                     VLVNGVSGVEVGTDLAWEILEKNKGPRVLFINRLDKEHANFSKVVAHAQEAYGFSAIP
                     VQFPVKEGETFDAIIDLLTMKKLTFQADGSGKYKSEEIPADLQGKAEEMRNALIEKIA
                     EADDAILEKYLNTMELSPDEITQGLRAGIRNATIFPILCGSAAKNIGSAPLLDFICEY
                     GPSPSDRPAALAADNTGAEVKVATDDKAPFSGFIFKTLSEMHVGELSLIRVYSGQIKA
                     GDEVLNTSNNITEKIGQIYELNGRNRKEKGNLIAGDIGALVKLRNTHTNNTLSDRRKQ
                     VVFPAVIFPEPVVQVAVTPKSKGDEDKISSGLHTLNETDPSFTIEVNSELKQTVLSGQ
                     GEIHLAVIVNRLKERYGVEVEQKKPRIPYRETITGKSDEKYRHKKQTGGAGQFAEVWM
                     RIEPMPRGGGFEFESQIVGGSISGPFIPSIEKGVRQILEEGAIAGYRIVDVKAIVYDG
                     KEHPVDSKDIAFQIAGREVFKMCVQSAKPILLEPIYDVEIKCPEENMGDIMGDLNSRR
                     GRIMGMESTGRFQVVKAKIPLAELHGYSSTLRSLTQGRANYSRKFSHYDPMPKEVEAK
                     VIAEAQAEKAAAQ"
     gene            3944..4417
                     /locus_tag="PKM23_10360"
     CDS             3944..4417
                     /locus_tag="PKM23_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNY91917.1"
                     /translation="MKRLWAPWRMEYIQQEKPAGCIFCDKPGEERDRENLILHRGRHC
                     FVIMNFYPYNNGHLMVVPFRHTADLAGLQKEEQLEMMELLGRCTRILAEAMKSQGFNV
                     GMNLGRIAGAGVDDHLHFHIVPRWNGDTNFMPVAGNTRVLSQGLTESWELLRRFF"
     gene            complement(4414..5193)
                     /gene="mazG"
                     /locus_tag="PKM23_10365"
     CDS             complement(4414..5193)
                     /gene="mazG"
                     /locus_tag="PKM23_10365"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNY91918.1"
                     /translation="MRSFARLVEIMAQLRGEEGCPWDREQTHASLRQHLLEESYEVIE
                     TIDEGRYDDLAGELGDLLLQVVFHAQMATEAGRFDIGDVVQQINEKLIRRHPHIFGDT
                     VINNAAEQTVAWEQSKLRKEGKRSAIGGVPRELPALIRAYRIQGKAAAVGFDWREAGP
                     VWDKVREELAELEEAAASAESGRISDELGDLLFSIVNLSRFLHINPEDALRGTIDKFT
                     RRFQEVEEDFRSRGRTLDGATLEEMDLVWEEVKERERRGKA"
     gene            5377..>6031
                     /locus_tag="PKM23_10370"
     CDS             5377..>6031
                     /locus_tag="PKM23_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNY91919.1"
                     /translation="MTFRERLQESRVVIFDGAMGTQIQGMHITDEEWAGVAGCNEILN
                     FTAPDKIASIHAAYFAAGAEVVETNTFGASRIVLAEYGLEEETRAINRAAAEIARRAA
                     AAAAGAKPRFVAGSIGPGTRLISLGQTSFSALHASCLEQALGLIEGGADLLLIETCQD
                     LLQIKTALIACRDAMTRLGRDLPLAVSVTVETTGTLLVGSEISAVLAALAPFEIDILG
                     "
BASE COUNT         1205 a         1988 c         1710 g         1128 t
ORIGIN      
        1 ctcttgacat tttcattgtt tagaagtaat tttccaggat ataaaacaag gcaacgccgc
       61 gcataagggt ctcacagtga gccattcctg tccgtttttt tggcatagag ctcgcagagc
      121 ctgcatcggc ttgtattatt tatttataat attatcatat ttttataggt atatttattg
      181 ccgactctca atgcccatct agagaaagtt gcaaatagag tgccggaaaa ccccgccatg
      241 gttttcggca gccaaagagt tgattaccgc cggtttcacc aggctgtgcg ccgtctggcc
      301 ggagctttgc aggccctggg gatcggacgg ggcgatcgcg tcgcgctgca tctgcccaat
      361 ctcccccatt tctgcatcag ctattacgcc ctccttcacc tcggcgcggt ggtcgtgccg
      421 ctttccatcc tgagcaaaga atccgaactc gcggccatac ttcagcacag tggctgccgg
      481 gggatcattt cctggtccgg catgctcgca cccctgcagg gcgccctcga gcaggcccgg
      541 gagtgcaccc tgcagctcgt gctgggcgaa cagattccgc gccatgccca ctccctcacc
      601 cggttgatcg cccagtcgcc agaactgacc agcgcgcccg ccgccggccc cgatgacctg
      661 gccgtcatca actatatcgc cggcggcgcc gaggagaacc tgggcgccga gttcacccag
      721 gctgccctgg ttgccggcgc gaccaccagc tccgagatgt tccgcattac cgttgacgac
      781 cgggtgctgg cggtgcttcc cctctttcat cccctcgggc agagtctggt catgcacggg
      841 gctttttacg ccggagcgac cgtggtgctg cagccgagat tccaggccag tgaagtagta
      901 acggccctgc gcgagcatca ggtcaccttc atggcggggg ttccggggat gttcaagctc
      961 ctcgcacaga tggatcctcc cgatcctccc ctcagcaccc tgcgttacgc cctcagctac
     1021 gggggacagc tccaggaggg ggtgctggaa acctttgagg agaaatttca cgcgcatctg
     1081 ctcgagagct acggttttac cgaggccggc cccctggtca cctcgatgcg catcaatcgt
     1141 gatcgcaagc ccggatcggt cgggctgccc atggtcggcg ttgagctgca gattctcgat
     1201 gaggaggggc gtctgctccg ccccaaccag agcggcgaga tctgggtcaa gagcccgagc
     1261 ctgatgcagg agtattaccg caagccggac cggacccagc agcggcttcg cgacgactgg
     1321 tttttctccg gggatgtcgg ctatctcgac gacgagcact acctcttcgt tcaggaacgc
     1381 aaggaggata tcatcaccaa ggggggcttt cagatcttct ccttcgaagt ggaggaggtg
     1441 ctgctcggtc atccggcggt ggccgaggcc gcggtggtgg gggttcccga tcccgcacag
     1501 gggcaggagg tcaaggcctg cgtagtgctc aaggcggggg agcaggtctc gcgcgatgag
     1561 ctgatcagct ggtgccgctc ctccttgccg gtctacaaga gcccgaagat aatcgagttc
     1621 gttcccgtcc tgcccaagag cccgaccgga cgggtgctca aaaatatatt gcgcgccgag
     1681 gccaggtctc cggcctccgc tcatggcaaa caatagctca tttcatcata cactgcgatc
     1741 ccccgcttca ggcggggctt aacaaaagga gaatcatcgt gaaagattac ggccccaacg
     1801 atatccgcaa cgtcggcctc gtctcacacc aatcaacagg caagagcacc ctggttgaag
     1861 gcatgctctt cgcagccggc gccatcaccc gtctcggcac catcgacgac ggctccacca
     1921 cctccgatta ccgtcctgac gagatcgacc gcaagatctc catcagcgcg gccctgatga
     1981 ccatcgactg gaaaaagaac aaattcaatc tcctcgacct ccccggctat cccgatttca
     2041 tcggcgaggt gcactgcgcc ctgcgcgtca ccgacctgac tatcgtgctc gtgaacggcg
     2101 tctccggagt cgaagtgggc accgatctgg cctgggaaat cctcgaaaag aacaaggggc
     2161 cgcgcgttct cttcatcaac cgccttgaca aggaacatgc caatttcagc aaggtcgttg
     2221 cccatgccca ggaggcctac ggtttcagcg ccatcccggt ccagttcccg gtcaaggaag
     2281 gcgagacctt tgatgcgatc atcgatctgc tcacgatgaa aaagctgacc ttccaggctg
     2341 acggcagcgg caagtataaa agcgaggaga tccctgccga tctgcaaggc aaggccgaag
     2401 agatgcgcaa tgccctgatc gaaaagatcg ccgaggccga cgacgccatc ctggaaaagt
     2461 atctcaacac catggagctc tcgcccgatg agatcaccca ggggctgcgc gccggcatcc
     2521 gcaacgccac catcttcccg atcctctgcg gctccgccgc gaaaaatatc ggcagcgccc
     2581 ccctgctcga ctttatctgc gagtacggcc cctccccgag cgatcgcccg gcagccctcg
     2641 ccgccgacaa caccggagcc gaggtcaagg ttgccacgga tgacaaggcc cccttcagcg
     2701 gtttcatctt caagaccctc tccgagatgc acgtcggcga gctctccctc attcgcgtct
     2761 actccggcca gatcaaggcc ggcgacgagg tgctcaacac ctccaacaac atcacagaaa
     2821 aaatcggcca gatctacgaa ctcaacggcc gcaaccgcaa ggagaagggc aacctcatcg
     2881 ccggggatat cggcgccctg gtcaagctgc gcaacaccca caccaacaac accctctccg
     2941 accgccgtaa acaggtggtc tttccggcgg tgatcttccc cgagccggtg gtccaggttg
     3001 ccgtcacccc caagagcaag ggcgatgaag acaagatctc gagcggactg cacaccctca
     3061 acgagaccga tcccagcttc accatcgagg tcaattcaga gctcaagcag accgtgcttt
     3121 cgggccaggg cgagattcac cttgcggtca tcgtcaaccg cctcaaggag cgctacgggg
     3181 tcgaggtcga acagaaaaag cccagaatcc cctatcgcga gaccatcacc ggcaagtccg
     3241 atgagaagta ccgccataag aagcagaccg gcggcgccgg ccagtttgcc gaggtctgga
     3301 tgcgcatcga gcccatgccc cgcgggggcg gattcgagtt cgaaagccag atcgtcggcg
     3361 gctccatctc cggccccttc atcccctcca tcgagaaggg ggtgcgccag atcctcgaag
     3421 agggggcgat cgcgggttat cgcatcgtcg acgtcaaggc catcgtctac gacggcaagg
     3481 agcatccggt cgattcgaag gatatcgcct tccagatcgc cggccgcgag gtcttcaaga
     3541 tgtgcgtcca gtcggccaaa ccgatcctgc tcgagcctat ctatgatgtc gagattaagt
     3601 gcccggagga gaacatgggc gacatcatgg gcgacctcaa cagccgccgc ggccgcatca
     3661 tgggcatgga atcgaccggc cgcttccagg tggtcaaggc caagattccc cttgcggaac
     3721 tgcacggcta cagcagcacc ctccgctccc tgacccaggg gcgcgccaat tattcacgca
     3781 agttctcgca ctatgacccc atgcccaaag aggtagaggc caaggtcatc gccgaggcgc
     3841 aggccgaaaa ggccgccgcg cagtaagcgt gatggggtga aaccatcagg ccctcccgcg
     3901 ccggccggcg caggagggcc ttttttaatg cgaaggagag atcatgaaga ggttgtgggc
     3961 gccatggcgc atggagtata ttcagcagga aaaaccggcg ggctgcatct tttgtgacaa
     4021 acccggggag gagcgcgacc gcgagaacct catcctccac cgcggccgtc actgttttgt
     4081 catcatgaat ttctatccct acaacaacgg ccatctcatg gtcgtgccct tccgccatac
     4141 cgccgatctg gccgggctcc agaaggagga gcagctggag atgatggagc tgctcggccg
     4201 ctgcacccgc atcctggccg aggcgatgaa aagccagggc ttcaacgtcg gcatgaacct
     4261 cggccgcatc gccggagccg gggtcgatga ccacctccac ttccatatcg tgccgcgctg
     4321 gaatggcgat accaacttta tgcctgtggc cggcaacacc cgtgtgctgt cacagggact
     4381 gacggaatcc tgggaactcc tccgccgctt tttctaggcc tttcctctcc gttcccgctc
     4441 cttgacctct tcccagacca ggtccatctc ctcgagggtg gcgccgtcga gggtgcggcc
     4501 gcggctgcga aagtcttcct ccacctcctg gaagcgccgc gtgaacttgt caatggtgcc
     4561 gcgcagggca tcctcgggat tgatatggag aaagcgcgag aggttgacga tggaaaagag
     4621 cagatccccg agttcatcgc tgatgcgacc gctctcggcc gaggccgcgg cctcctcgag
     4681 ttcggcgagc tcctcgcgga ccttgtccca gaccgggccg gcctcgcgcc agtcgaatcc
     4741 caccgccgcc gccttgccct ggatgcggta ggcgcggatc agggcgggca gctcgcgggg
     4801 caccccgccg atcgccgagc gcttgccctc cttgcggagc ttggactgct cccaggccac
     4861 cgtttgttcg gcggcattgt tgatgaccgt atccccgaag atgtggggat ggcggcggat
     4921 cagcttttcg ttgatctgct ggaccacatc cccgatatcg aaacgccccg cctccgtggc
     4981 catctgggcg tgaaatacca cctggagcag gagatcaccg agttccccgg ccaggtcgtc
     5041 gtagcgcccc tcgtcgatgg tctcgatgac ctcgtaggac tcttccagca ggtgctggcg
     5101 cagggaggca tgggtctgtt cgcggtccca gggacagccc tcttctcccc gcagctgcgc
     5161 catgatctcc accagccgtg caaaactccg catatccccc ctgctttgct cctctgccat
     5221 aatctctgcg catcctttct gattaaatcc atgctccagc gaggcaaaat atagtcattt
     5281 ttccgctgtc ccgcaatctt tctccgatta ttaccttgaa ataatctcga tattttagta
     5341 gcttggtaaa aagcaaccct taagaggatg gacgagatga cctttcgcga acggctgcag
     5401 gaatcccggg tggtgatctt cgacggggcc atgggcacgc aaatccaggg catgcatata
     5461 accgacgaag agtgggcggg tgtggcgggc tgcaatgaga tcctcaactt caccgcgccg
     5521 gacaagatcg cctccatcca cgccgcctac ttcgccgccg gggcggaggt cgtcgagacc
     5581 aacaccttcg gagccagccg catcgtcctg gccgagtatg gcctcgagga agagacccgc
     5641 gccatcaacc gcgccgcagc ggagatcgcc cgccgggccg ccgcagcagc cgccggagca
     5701 aaaccccgct tcgtcgccgg ctcgatcggc cccggcaccc gcctgatttc cctcggccag
     5761 accagcttca gcgctctgca cgcctcctgc ctggaacagg ccctgggact gatcgagggc
     5821 ggcgccgacc ttctcctcat cgagacctgc caggacctgc tccagatcaa gaccgccctc
     5881 atcgcctgcc gagatgccat gacccggctc ggacgcgatc tcccactcgc cgtctcggtg
     5941 acggtcgaga ccaccggcac cctcctggtc ggctccgaaa tcagcgccgt cctggccgcc
     6001 ctcgctccct ttgaaatcga catcctcggc c
//
LOCUS       DAOPWU010000205         6016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13098_length_6016_cov_3.751216, whole genome shotgun sequence.
ACCESSION   DAOPWU010000205 DAOPWU010000000
VERSION     DAOPWU010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13098_length_6016_cov_3.751216"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKM23_10375"
     CDS             <1..287
                     /locus_tag="PKM23_10375"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY91920.1"
                     /translation="SFNGDDYFLAAAQGGMAACLEAEKKYNEAAETYLKAVDAYSSVL
                     DPSMLLNAGRCFNEAGNKEKAKKALNRIIEEHPKAAEKDDALLLIGLLNG"
     gene            388..840
                     /gene="rimP"
                     /locus_tag="PKM23_10380"
     CDS             388..840
                     /gene="rimP"
                     /locus_tag="PKM23_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNY91921.1"
                     /translation="MTQVNELKNLIEPVLAGMGIDLVDLEIHGRKGSLLLRIYADEIG
                     GISLERCASASLAVSDCLDQNGLLEGAYRLELSSPGTDRPLTTERDFQRHCGRKVKLT
                     YRDNDQVQDASGRIEEAAGGLLQLQTGTDLLTIPLGQVVLAKIIIEFK"
     gene            860..2104
                     /gene="nusA"
                     /locus_tag="PKM23_10385"
     CDS             860..2104
                     /gene="nusA"
                     /locus_tag="PKM23_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNY91922.1"
                     /translation="MKNEIVEAFSLLVKEKNIDKEVLSRILQEIFLGLLKKKYGPNGN
                     YSIFVNMEKGEIEIYQSKNVVDEVLDEVTEIALDEAQKSAPDMEVGDEYLEIIDPSTF
                     GRRLIISAKQNLNQRIKEIEKELVYEEYKNRVGEIIIGEIRQVNRDEIFMTVDNRTEV
                     VLPKSEQIKDERHHRGGSLRAVIKEVRKSNRGPEIVVSRSDDMFITRLFENEVPEIDE
                     GIIEVRGIAREAGERTKIAVYSHDKRIDAVGACVGMKGMRIQSIVKELNNEKIDIINW
                     SSEPEIFITRALSPAKPLRIIIDEEEKKVSAVLDDEQISLAIGKGGVNRRLASRLTGY
                     DIQTMREEDFRKLMDEQRDLDRSIEDVTSISDKQKAILIGGGYSTVRDVLNAGADILT
                     QLPGVGLKTAEKIIAALEAETE"
     gene            2174..4846
                     /gene="infB"
                     /locus_tag="PKM23_10390"
     CDS             2174..4846
                     /gene="infB"
                     /locus_tag="PKM23_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNY91923.1"
                     /translation="MKMRVFQVAREFNISNEALVAFLTKLGHDVRNQMSLVSDEAYAL
                     VVDKFGEKTSQVDEEYEFRRRLREKKAQEEAKKAEAQKNLERRLAVAQEMAKEMPELR
                     KRREASVVEAAAAKQERAAELAKKAEAAALAAAQPPAKGEETAPAAAVEAPAETVRTK
                     PRKKLVVIEIPPEETARRPREKERPAEETAKPAARPTKEGGAPKEMVPVARLEEMEAE
                     EGRHKKKRKRKRKKKGAETPAPAVQPAVEAEHKRKRPKKKKKITFNEAEIQETIRQTM
                     AAMEEPGKAKKKKRRTQADDEIEVEETPIIKVSEFMSVSELAKLMEAEASEIIRKCME
                     LGMMVSINQRLDMDTITLLAGEYGYEVAPLAEFGEDLLEQMEKEESEDEANLVRRPPV
                     VTIMGHVDHGKTSLLDHIRKSNIIAGEAGGITQHIGAYEVKTDDGKPITFLDTPGHEA
                     FTAMRARGAQATDIVILVVAADDNVMPQTIEAISHAKAANVPIVVAINKIDKPGANPD
                     NIRMQLSKHDVLVEQWGGKHQCVEISAKTGQNIELLLDNVLLEAEVLDLKANPERLAR
                     GVVIESALDRGKGVVATVLVQSGSLKVGDPFIAGSYSGKVRSMFDDHGRKVKQAGPSM
                     PVAVLGFAGMPQAGDSIIVLSSERDTREISQKRQQLQREHERWRSHARTLDEISRQIQ
                     KGQIRQLFVVLKADVDGSIEAITDTLINLGTDEVAVEIIHKGVGAISESDVLLASASN
                     AIIIGFNVRPTTKAREMASKENIDIRLYEVIYDIVNDIKSALEGFLAPTISEELTATV
                     EIRQVFKVPKVGNIAGCYVSSGKISRSDRVKVYRDDRLIHDGQLSSLKRFKDDVREVA
                     TGFECGLALANYDDIKVSDIIEAYRMVETKRTLE"
     gene            4862..5143
                     /locus_tag="PKM23_10395"
     CDS             4862..5143
                     /locus_tag="PKM23_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNY91924.1"
                     /translation="MLVGLLQIELFIPESGSLKGKRFLLKSIKTRIHNKFNVSVAEVD
                     ENEKWQRTTLGIALVANERKFIDQVMSEVIRLIETEDEVEIIGQQLEIV"
     gene            5147..5503
                     /gene="rbfA"
                     /locus_tag="PKM23_10400"
     CDS             5147..5503
                     /gene="rbfA"
                     /locus_tag="PKM23_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNY91925.1"
                     /translation="MNKYKRADRVAALLQEEVSNYLLFEMKNADLGFITITRVRVSDD
                     LRHARIFYSVLGEQERIDLTAKKLEQLTGPIRGEMGHRLGLRYVPEIEFSYDDSAAYA
                     AHIQEVIEKIHREEKS"
     gene            5608..>6016
                     /gene="truB"
                     /locus_tag="PKM23_10405"
     CDS             5608..>6016
                     /gene="truB"
                     /locus_tag="PKM23_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNY91926.1"
                     /translation="MKKHLRGGKAGHAGTLDPFADGVLLVCTGRETRTITGLVDAEKE
                     YQARLRLGMETDTLDISGTICARREWRLQEVAAVQEIADRFVGEIEQVPPVFSAIKLG
                     GQKMYDLARRGRQMDLPARRVSIHSIEIMEFNET"
BASE COUNT         1475 a         1761 c         1711 g         1069 t
ORIGIN      
        1 cctctttcaa cggggacgat tattttctag ccgccgccca gggcggaatg gccgcctgcc
       61 tcgaagctga aaagaaatat aacgaagccg ccgaaaccta cctcaaggcc gtcgatgctt
      121 actcttcggt gctcgatccc tccatgctgc tcaacgccgg acgctgcttc aatgaagccg
      181 gcaacaagga aaaggctaaa aaggccctca accgcatcat cgaggaacac cccaaagccg
      241 cagaaaagga tgacgccctg ctgcttattg gtcttctcaa tggatgattt tgcgaatttt
      301 cttgcatagt atgttatttt tttatatatt atctatctga agtgggccct ttctggccca
      361 cttttttgtt gtaaaagggt gtcagatatg acgcaagtta atgaattaaa aaatcttatc
      421 gagccggttc tcgccgggat gggcatcgac ctcgtcgacc tcgaaatcca tggccgcaag
      481 ggcagccttc tcctccgcat ctatgccgat gaaatcggag ggatctctct ggagcgctgc
      541 gccagcgcca gtctcgctgt gtccgattgc ctggaccaga acggcctcct cgagggcgcc
      601 tatcggcttg aactttcgtc acccggaacc gaccggccac tcaccacgga acgggatttt
      661 caacgccact gcggccgaaa agtcaagctg acctatcgcg acaatgacca ggttcaagac
      721 gccagcgggc ggattgaaga agcagcaggg ggactcctgc agctgcaaac cgggacggac
      781 cttttaacca tcccgctcgg gcaagtcgtt ttggccaaaa ttatcatcga attcaagtga
      841 tctcaattgg aaaacagcca tgaaaaatga gattgtcgaa gccttctccc tgcttgtcaa
      901 agagaaaaac attgacaagg aggtgctctc acggatcctg caggaaatct ttctcggcct
      961 cctgaaaaag aaatacggcc ccaacggtaa ctactccatt tttgtcaata tggagaaggg
     1021 ggaaatcgaa atctatcaga gcaagaatgt cgtcgatgaa gtgctcgatg aggtgaccga
     1081 gatcgccctc gatgaggccc agaaaagcgc cccggacatg gaggtcggcg atgaatacct
     1141 cgaaatcatc gatccctcca ccttcggacg gcggctgatc atctccgcca aacagaacct
     1201 caatcaacgc atcaaggaga tcgagaagga actcgtctac gaagagtaca agaaccgggt
     1261 gggcgagatc atcattggcg agatccgcca ggtcaaccgc gatgagatct ttatgacggt
     1321 cgacaaccgc accgaggtcg tcctgcccaa gagcgaacag atcaaggatg aacgccacca
     1381 ccgcggcggc agcctgcgcg cagtcataaa ggaggtgcgc aagagcaacc gcggtccgga
     1441 aatcgtcgtc agccgctccg atgacatgtt catcacccgc ctcttcgaga acgaagtgcc
     1501 cgaaatcgat gaaggcatta tcgaagtgcg cggcatcgcc cgcgaggccg gagagcgcac
     1561 caagatcgcc gtctattccc acgacaagcg catcgacgcc gtcggcgcct gcgtcggtat
     1621 gaagggcatg cgcatccagt ccatcgtcaa ggaactcaac aacgaaaaga ttgacatcat
     1681 caactggagc agcgagcccg aaatttttat cacccgcgca ctgagtccgg ccaaaccact
     1741 gcgcattatc atcgatgaag aggagaaaaa ggtcagcgcg gtccttgatg acgaacagat
     1801 ctccctggct atcggcaagg ggggcgtcaa ccgccgtctc gcctcccgcc tcaccggata
     1861 tgatatccag accatgcgcg aagaggactt ccgcaagctc atggacgaac agcgcgatct
     1921 cgatcgttct atcgaggatg tcaccagcat ctccgataag caaaaggcca tccttatcgg
     1981 cggcggctac agcaccgtgc gtgacgtcct caatgccgga gccgatattc tcactcagct
     2041 gccgggtgtg ggcctcaaaa ccgcggaaaa gatcatcgcc gcactggagg ccgagaccga
     2101 ataaggctgg cttgtcagtc gactgtttca ccagggtgat cgatatgcag aactgaggag
     2161 tgttaatttg tctgtgaaaa tgcgggtctt tcaggtagcg cgagagttca acatctcaaa
     2221 tgaggctctt gtcgcctttc tcaccaagct cggccatgat gtccgcaacc agatgagtct
     2281 ggtctcggat gaggcctatg cccttgtggt ggacaaattc ggcgaaaaaa catcgcaggt
     2341 cgatgaagag tacgaattcc gccgcagact gcgtgagaag aaggcgcagg aggaggccaa
     2401 aaaggccgaa gcccagaaaa atctcgagcg tcgtcttgcc gtcgcccagg agatggccaa
     2461 ggagatgccc gagctccgca aacggcgtga ggccagtgtg gtcgaagccg cagccgccaa
     2521 gcaggaacgg gcggccgaac tggcgaaaaa ggccgaggcg gccgctcttg ccgctgccca
     2581 accgccggcc aagggtgagg aaaccgcgcc cgcagccgcc gttgaagcac cggccgaaac
     2641 cgtccggacc aagccgcgca agaaactggt cgtcatcgag atcccgcccg aggagaccgc
     2701 acgccgtccg cgtgaaaagg aacgtcccgc cgaagaaacc gcgaaaccgg cggcaaggcc
     2761 gaccaaggag ggcggcgctc ccaaggagat ggtcccggtt gcacgtcttg aggagatgga
     2821 ggctgaagag ggacgccaca agaagaagcg caaacggaag cgcaagaaga agggagcgga
     2881 gacccccgca ccggcggttc agcccgcggt cgaagccgaa cacaagcgca aacggccgaa
     2941 gaagaagaaa aagatcacct tcaatgaagc cgagatccag gagaccatcc gccagaccat
     3001 ggcggccatg gaggagcccg gcaaggccaa gaagaagaaa cgccgcaccc aggccgacga
     3061 tgagatcgag gtcgaggaga ccccgattat caaggtcagc gaatttatgt cggtctccga
     3121 actggccaaa ctgatggagg cggaggcctc agagatcatc agaaaatgca tggaactggg
     3181 catgatggtc tcgatcaacc agcgcctcga catggatacc atcaccctcc tggccggcga
     3241 atacggctat gaggtggcgc ctctcgccga attcggcgag gacctgctcg agcaaatgga
     3301 gaaggaggag tcggaggatg aggctaatct ggtgcgccgt ccaccggttg tcaccatcat
     3361 ggggcatgtc gaccatggca aaacctcgct gctcgaccat atccgcaaga gcaacatcat
     3421 cgccggcgag gccggaggca tcacccagca cattggcgct tatgaggtca agaccgatga
     3481 tggcaagccc atcaccttcc tcgacacccc gggacacgaa gccttcaccg ccatgcgcgc
     3541 ccgcggcgcc caggccaccg atatcgtcat cctcgtcgtc gcggccgatg acaacgtcat
     3601 gccgcagacg atcgaagcga tcagccacgc caaggccgcc aacgtcccga tcgtggtcgc
     3661 tattaacaag atcgacaagc ccggcgccaa tccggacaac atccgcatgc agctttccaa
     3721 gcatgatgtc ctggtcgagc agtggggcgg caaacaccag tgcgtcgaaa tctcggccaa
     3781 gaccggccag aatatcgagc tgctgctcga taatgtgctg ctcgaagccg aagtgctcga
     3841 cctcaaggcc aatccagagc gtctggcgcg cggggtggtc attgaatcgg ccctcgatcg
     3901 cggcaagggc gtcgtcgcca ccgtcctcgt tcagtccggc tccctcaagg taggcgatcc
     3961 ctttatcgcc ggctcttaca gcggcaaggt gcgttcgatg ttcgacgacc acggccgcaa
     4021 ggtcaaacag gccggcccct caatgccggt ggctgtgctc ggctttgccg gcatgcccca
     4081 ggccggtgac tcgatcatcg tgctcagctc agaacgcgat acccgcgaga tcagccagaa
     4141 gcgtcagcag ctccagcgtg aacacgaacg ctggcgcagc cacgcccgca ccctggacga
     4201 aatctcgcgc cagatccaga agggccagat ccgccagctc tttgtcgtcc tcaaggccga
     4261 cgtggacggc tccatcgagg ccatcaccga taccctgatc aatctcggca ccgacgaagt
     4321 cgccgtcgaa atcatccaca agggcgtcgg cgccatctcc gagtcggatg tcctgctcgc
     4381 ttcggcctcc aacgccatca tcattggctt caatgtgcgg ccgaccacaa aggcgcgcga
     4441 aatggcctcg aaggagaaca tcgacatccg cctctacgaa gtcatctatg atattgtcaa
     4501 cgatatcaag tccgccctgg aaggattcct cgccccgacc atcagcgagg agctgacggc
     4561 gacggtcgag attcgccagg tcttcaaggt gcccaaggtc ggcaacatcg ccggctgtta
     4621 cgtctcctcg ggcaagatca gccgcagtga ccgggtcaag gtctatcgcg acgatcgcct
     4681 gatccatgac ggtcagctct cttccctcaa gcgcttcaag gacgatgttc gcgaagtggc
     4741 gacgggattc gagtgcggcc tcgccctggc caattacgac gatatcaagg tcagcgatat
     4801 catcgaggcc taccgcatgg ttgaaaccaa gcgcaccctg gaataggaga ggggagacgg
     4861 catgctggtc ggacttttgc agatagaact cttcatcccc gagagcggct cgctcaaggg
     4921 gaaaaggttt ctcctcaaaa gcatcaagac caggattcat aacaagttta atgtctccgt
     4981 ggctgaagtg gatgagaacg agaaatggca gcgcaccacc ctggggatcg ccctggtcgc
     5041 caacgagcgc aagtttatcg accaggtgat gtcggaggtt attaggctga tcgaaaccga
     5101 ggatgaggtt gaaatcatcg gacagcagtt ggagatcgtc tagagaatga acaagtataa
     5161 acgcgcagac cgggtagccg ccctcctcca ggaagaggtc agcaattacc tgctcttcga
     5221 gatgaaaaat gccgatctgg gcttcatcac catcacccgg gtccgggttt ccgacgatct
     5281 gcgccatgcg cgcatctttt acagcgtcct cggtgaacag gaacgcatcg atctaaccgc
     5341 aaagaaactg gagcagctca ccggccccat tcgcggcgaa atggggcacc gactcggcct
     5401 gcgctatgtg cccgagatcg agttttcgta tgatgattcc gctgcttacg cagcccatat
     5461 ccaggaagtg atcgaaaaga ttcaccggga ggaaaagtca taaccgccca tgcaaccgcg
     5521 ggttcagcac gggaacattt ttgcccggat ctcgttctca atctgcgcaa gcccatcggc
     5581 tggtcttcct ttgatgtcgt gcggctggtg aaaaagcatc tacgcggcgg caaggccggt
     5641 catgccggca cccttgatcc attcgctgat ggcgtgctgc tggtctgtac aggccgtgaa
     5701 acccgcacca tcaccggact ggtcgatgcc gaaaaggaat atcaggcacg gctgcggctg
     5761 ggcatggaga ccgacactct cgatatcagc ggcacgattt gcgcacgccg ggagtggcgc
     5821 ctgcaagagg tcgcggccgt gcaggagatc gcagaccgct tcgtcggcga gatcgaacag
     5881 gtgccgcccg ttttttcggc gatcaagctg ggcggacaga aaatgtacga tcttgcgcgc
     5941 aggggaaggc agatggacct gccggccagg cgggtgagca tccactccat tgaaataatg
     6001 gaatttaacg agacgg
//
LOCUS       DAOPWU010000206         6002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13156_length_6002_cov_4.899613, whole genome shotgun sequence.
ACCESSION   DAOPWU010000206 DAOPWU010000000
VERSION     DAOPWU010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13156_length_6002_cov_4.899613"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PKM23_10410"
     CDS             complement(<1..558)
                     /locus_tag="PKM23_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY91927.1"
                     /translation="MIELVQVNKSYSGKVKAVDNLTLTVPAGEIVGFLGPNGAGKTTT
                     LKMLTGILNPDSGSIRINGLELHREPIRVKEQIGFVPDSPDIFLRLKGIEYLRFMADL
                     YRVGGAERRQRIEEYTARFEMTEVLGNKIQSYSHGMRQKIVLIGALLHDPAVWILDEP
                     LTGLDPKSSFLLKEILRSEAGKGKTV"
     gene            803..2209
                     /locus_tag="PKM23_10415"
     CDS             803..2209
                     /locus_tag="PKM23_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91928.1"
                     /translation="MANNLDELITYLQRLLGPEYEAFARAPLEPPSIRLNPLKGARRE
                     GLLQRFKAWGVAHQPHPVNPDGMMLEEDRLPLSHTLAYFAGDFVYQGLSSQLPVLALD
                     PRPGESVLDLCAAPGSKSTQIAALMQNRGRLLLTDSSTRRLQPLMANLFSAGVMNAVL
                     LNLPGQLIGRHFPEQFDRVLVDAPCSALANLPVRAFQSYWSEAFLRKISTIQEQLLIS
                     GIKAARVGGVIVYSTCSICPEENEMVLERILRRYPVELEPVAFAGAPCCRPPLESYLG
                     RRFDPAIRQAVRIHPWPLPFEGFFVARLRKTGPLPIRPVTEAMPWLQSRRGDDPEIAL
                     LLDHLEQLWGIPAGPLRDSRYLRSLHRLWMIAPEWERLPQRAFMMAGLPLADEKTTFW
                     RPTTPAVQWLGERIGKERISFTDAELAAFFHDGRLPAPAGPLRYHVLHADGRNLAIVA
                     QSEGFYRIHLPHRFDLVL"
     gene            2264..3856
                     /locus_tag="PKM23_10420"
     CDS             2264..3856
                     /locus_tag="PKM23_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY91929.1"
                     /translation="MTDRPEKKSKKGAITRRDLLKSAASLPVIGLLGHLSWKEHKLER
                     GKKERIFEALDLEKAALPQPQLSVRPKQRVRIGLIGFGARGEQLARALGFARPEWIAS
                     VREAAALNRLDTRLQDWLNQEDLQVAITAVCEVFDQRREQAAAAATSTLRPGTPASTA
                     ALPVPALCVDYHDLVNRSDVDAVIIAAPDFQHAPMALAAIAAGKHVYVEKCMTRTEEE
                     AFALRAAMKTSPVVFQLGHQYPQNACYVRAKEIIERNILGKITLIETSSNRNTPSGAW
                     VRHLDSQGRPRPGDPRSIDWQRWLGDCPQVPFSIDRFYNWTKWWDYATGLSGQLYCHE
                     VDMVNQLLGVGIPAAVMASGGIYFHKDGREIPDIWNVVCEYPDHDLTHLYSASLANSR
                     QRGRLFMGHDAAMEVGDSLQVYVDGDSSRYQASLRQGEMSPGQTIVSFRPGMDKLDAI
                     TSASEKFYAGRGLVYTWRDGRRVDTSHLHLREWLGCIRDGGTPSCSIAKAFATTITCH
                     MATRSYREQRRVTWDKEAERIV"
     gene            3987..5027
                     /gene="nadA"
                     /locus_tag="PKM23_10425"
     CDS             3987..5027
                     /gene="nadA"
                     /locus_tag="PKM23_10425"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNY91930.1"
                     /translation="MSNSSLFDQLYKKLKNVIPVAEIRYKAELAEQINRLKKERNAVI
                     LGHNYMEPALYHSVPDYRGDSLELSRRAAEAEADIIIFCGVRFMAETAKILNPNKTVL
                     IPSPRAGCSLAESITAEDVRQLRRLYPGVPVVTYINTYADVKAETDICCTSGNAVKVV
                     ESLKSDKVIFLPDRYLAGNVARETGKTIVFPVKSPAGLARPEPGIEYAMIGWDGRCEV
                     HDKFTVEDIENVRAQFPDVVILAHPECRPEVTAAADFSGSTSAMIRYVRETRAPHYLL
                     LTECSMGDNIAAENPDRELLRLCTVRCPHMAEITLEDTLESLRQTRYVVEVPEEIRVR
                     ALRAVTRMLEIG"
     gene            5033..5968
                     /locus_tag="PKM23_10430"
     CDS             5033..5968
                     /locus_tag="PKM23_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNY91931.1"
                     /translation="MSIPGSPALILGASGFLGEAFARHLQAAGEPAACLIHRSPLPAE
                     IRPVRLYHGSLLTFPWRILEADPPRAIIHFARLSAPSAAGRRLAAQASTMANRRLLAW
                     LERLAQPPRLVLIAGTLAYGPSAEEEIYEDHALHPAGFAREYARGEAPILAAMAAGRL
                     PVQVMRPAWVYGARSWLRGFFFTPMLRQGHVPLYGEGDNRMALIHVEDAAALIDFLSR
                     EAPAGESYNLLCGAPLRQAELAETLSRISGLPIKRITRRDFPSRDRGVWESLTFSQRT
                     ASRHAGLYQRHGFRHPDAGAALASLWRSFINTPSA"
BASE COUNT         1045 a         2106 c         1755 g         1096 t
ORIGIN      
        1 gaccgtcttg cctttgccgg cttcactgcg caggatctcc ttgagcagga aggacgactt
       61 gggatcgagg ccggtgaggg gttcgtcgag gatccagacc gccggatcgt gcaaaagggc
      121 gccgatgagg acgatctttt ggcgcatgcc gtgggaataa ctctggatct tgttgcccag
      181 cacctcggtc atttcgaacc gggcggtgta ttcttcgatg cgctgccggc gctcggctcc
      241 cccgacccga tagaggtcgg ccatgaagcg gagatactcg atgcccttga ggcggaggaa
      301 aatatccggg ctgtccggga caaaaccgat ctgttccttg acccggatcg gctccctgtg
      361 cagctccagg ccgttaatac ggatcgagcc gctgtcggga ttgagaatac cggtgagcat
      421 cttgagggtg gtggttttgc cggcgccgtt ggggccgaga aaaccgacga tctcgcctgc
      481 cggaacggtg agggtgagat tatcgacggc cttgaccttg ccgctgtagg atttgttgac
      541 ctggacgagt tcaatcatct gcctgctcct tatctggatc gagatcaaat ataatcaatc
      601 ctccatttaa atacacgcaa aattccgccc ctggccagga ttctcctccc ccccggagag
      661 ggtgcagcca ctttctggcg gcgtgcagcc tctgccgccg gcctcgcgca tttttttcca
      721 gcccgcatca gcagtcttga ctccttgctc cggtttgcct atattggttt gtgcgtatga
      781 tctgcaagcg ggtgcaaaaa gcatggcaaa caacctcgac gaactgatca cctatctgca
      841 gcggctgctc gggccggagt atgaggcttt tgcccgggcc cccctcgagc ctccctcgat
      901 ccgcctcaat cccctcaagg gggcccggcg tgaggggctg ctgcagcgct tcaaggcctg
      961 gggggtagca caccagcccc atccggtcaa tcccgacggc atgatgctgg aggaggaccg
     1021 tttgcccctg agccacaccc tcgcctattt cgccggggat ttcgtctatc aggggctttc
     1081 ctctcagctg ccggtgctgg ccctcgaccc caggccgggg gagagcgtgc tcgacctctg
     1141 cgccgctccc ggctccaagt cgacccagat cgccgccctc atgcagaacc gcggccgcct
     1201 cctgctcacc gacagctcca cccgccgcct ccagcctctg atggccaacc tcttcagcgc
     1261 cggcgtgatg aacgccgtgc tgctcaacct ccccggccag ttgatcggcc gccactttcc
     1321 tgagcagttc gaccgtgtcc tcgtcgacgc cccctgctcc gccctcgcca atctgccggt
     1381 gcgcgcattt caatcctact ggtccgaggc tttcctcagg aaaatcagca ccatccagga
     1441 gcagctgctg atctccggga tcaaggcggc cagggtgggg ggcgtcatcg tctactccac
     1501 ctgctcgatc tgccctgaag agaacgagat ggtgctggag cgcatcctcc gccgttatcc
     1561 ggtcgagctc gagccggtcg cctttgccgg ggccccctgc tgccggccgc ccctcgagag
     1621 ctatctcggc cgccgcttcg atccggctat ccggcaggcg gtgcggatcc atccctggcc
     1681 cctgcccttt gagggttttt tcgtcgcgcg gctgcgcaag accggccccc tccccatccg
     1741 gccggtgacc gaggccatgc cctggctgca gagccgtcgg ggcgacgacc ccgaaatcgc
     1801 cctcctgctc gatcatctcg agcagctctg gggcatcccg gccggacccc tgcgcgactc
     1861 gcgctacctg cgctcacttc atcgcctctg gatgatcgcc ccggaatggg agcggctgcc
     1921 gcagcgcgcc tttatgatgg ccgggctgcc cctggctgat gagaaaacca ccttctggcg
     1981 gccgaccaca cccgcggtgc agtggcttgg cgagcgcatc ggcaaggaga ggatctcttt
     2041 caccgatgca gagcttgccg cattcttcca tgatgggcgc ctgcccgccc ccgccgggcc
     2101 cctgcgctat catgtcctgc acgccgatgg acgcaacctg gccatcgtcg cgcagtcgga
     2161 gggattttac cgcatccatc tgccccaccg cttcgatctg gttctctgaa gcaagcagag
     2221 ccggcgaggg gttctgatga actgaccaaa cggagaaacg agcgtgacag acagaccgga
     2281 gaaaaaatcg aagaagggag ccatcacccg cagggatctg ctcaagagcg cggcctcgct
     2341 gccggtgatc gggcttttgg gccatctgag ctggaaggag cacaaactgg agagggggaa
     2401 gaaagagcgc atcttcgagg ccctcgatct cgaaaaggca gccctcccgc agccgcagct
     2461 cagcgtgcgg ccgaaacagc gggtgcgcat cggcctcatc ggcttcgggg cgcgcggcga
     2521 gcagctggcg cgggccctgg gtttcgcacg accggaatgg atcgcctcgg tcagggaggc
     2581 agccgcactc aaccgcctcg atacccgcct gcaggactgg ctcaaccagg aggatctcca
     2641 ggtcgccatc accgccgtct gcgaggtctt tgaccagcgc cgcgagcagg ccgcggctgc
     2701 agccacttcc accctgcgac ccggaacgcc cgcctccacc gctgccctgc ccgttcccgc
     2761 cctctgcgtc gactatcatg atctcgtcaa ccgcagcgac gtcgacgccg tgatcatcgc
     2821 tgcacccgat tttcagcacg cccccatggc cctggccgcc atcgccgccg gcaagcatgt
     2881 ctacgtcgaa aaatgcatga cccgcaccga agaggaggct ttcgccctgc gcgcggcgat
     2941 gaaaaccagc cccgtcgtct ttcaactcgg ccaccagtat ccccagaacg cctgctatgt
     3001 ccgcgccaaa gagatcattg aacgcaatat cctcggcaag atcacgctca tcgagacctc
     3061 cagcaaccgc aacaccccca gcggcgcctg ggtgcgccac ctcgacagcc agggccggcc
     3121 gcgccccggc gacccccgct ccatcgactg gcagcgctgg ctgggtgact gcccgcaggt
     3181 cccgttcagc atcgaccgct tttacaactg gaccaagtgg tgggactatg ccaccggact
     3241 tagcggccag ctctactgcc atgaagtgga tatggtcaac cagctcctcg gcgtcggcat
     3301 cccggctgcg gtgatggcct ccggaggcat ctatttccac aaggacggcc gcgagatccc
     3361 cgacatctgg aacgtcgttt gcgagtatcc cgaccacgac ctgacccatc tctactccgc
     3421 cagcctggcc aacagccgcc agcgcggccg cctcttcatg ggccacgacg ccgccatgga
     3481 agtgggcgac agcctgcagg tttacgtcga cggggactca agccgctacc aggccagcct
     3541 gcgccagggg gagatgagcc ccggccagac catcgtctca ttccgcccgg gcatggacaa
     3601 gctcgatgcc atcacctcgg cgagcgaaaa attctacgcc ggccgcggcc tggtctatac
     3661 ctggcgcgac ggccgccgcg tcgacacctc ccacctccat ctcagggagt ggctgggctg
     3721 cattcgcgac ggcggcaccc cgagctgctc catcgccaag gccttcgcaa caacaatcac
     3781 ctgccacatg gccacccgct cctatcgcga acaacgccgg gtgacctggg acaaggaggc
     3841 cgaacgcatc gtctgaggcg ctgcagccgg cgccgtcagc acccttgtat ttgtagtaaa
     3901 aattggttat tttttacatt gtggaaccag aaaagctcca ctgaggttac acgctcgtat
     3961 ctgtcaaacg tgaatgagga tccaagatga gtaatagcag ccttttcgat cagctttata
     4021 aaaagctcaa gaatgtcatc cctgttgcag agatccgcta caaggcggag ctggcggagc
     4081 agatcaaccg gctcaagaag gagcgcaatg cggtcatcct cggccacaac tatatggagc
     4141 ccgccctcta ccattcggtc ccggattacc gcggggattc cctcgaactg agccggcggg
     4201 cggccgaagc cgaggccgac atcatcattt tctgcggcgt ccgcttcatg gccgaaaccg
     4261 ccaagatcct caaccccaac aagaccgtgc tcatcccctc gcccagggcg ggctgctccc
     4321 tggccgagag catcaccgcc gaagatgtcc gccagctgcg gcgcctctac cccggggttc
     4381 cggtggtcac ctacatcaac acctacgccg acgtcaaggc cgaaaccgac atctgctgca
     4441 cctcgggcaa cgccgtcaag gtggtggagt cgctcaaatc ggacaaggtg atctttctcc
     4501 ccgaccgcta tctggccggc aatgtcgccc gcgagaccgg caaaaccatc gtctttccgg
     4561 tcaagagtcc ggccggcctg gccagacccg agcccggcat cgagtatgcc atgatcggtt
     4621 gggatggccg ctgtgaggtc cacgacaaat tcacggtcga ggatatcgag aatgtccgcg
     4681 cccagttccc ggatgtggtc atcctggccc atccggagtg ccgtcccgaa gtgactgcgg
     4741 ccgccgattt ctccggcagc acctcggcga tgatccgcta tgtgcgcgag accagggccc
     4801 ctcactatct gcttctgacc gagtgcagca tgggcgacaa tatcgccgcc gagaatcccg
     4861 accgcgagct gctgcgcctg tgcacggtgc gctgtccgca catggccgag atcacccttg
     4921 aggataccct ggaatccctg cgccagaccc gctatgtggt cgaggtgcct gaggagatcc
     4981 gggtgcgcgc cctgcgcgcg gtgacccgca tgctcgagat cggttgaggc ggatgagcat
     5041 tccgggctcc cctgctctca tcctcggcgc cagcggcttt ctcggcgagg cctttgcccg
     5101 ccacctccag gcggcaggcg agcccgccgc ctgcctcatc catcgctccc ccctgccggc
     5161 ggagatcagg ccggtacggc tctatcacgg ctccctgctc acctttccct ggcggatact
     5221 ggaggcggat cccccccgcg ctatcatcca tttcgcccgt ctttcggccc cctcggcagc
     5281 ggggcggcgt ctggccgccc aggcgagcac catggccaac cgccgcctgc tcgcctggct
     5341 ggagaggctc gcgcagccgc cgcggctggt cctgatcgcc ggcaccctcg cctacggtcc
     5401 atccgccgaa gaagaaattt acgaggacca tgccctccat ccggcgggat tcgcccgcga
     5461 gtatgcgcgc ggcgaggccc ccatccttgc cgccatggca gccgggcggt tgccggtaca
     5521 ggtcatgcgt ccggcctggg tctacggcgc gcgttcgtgg ctgcgcggtt tctttttcac
     5581 cccgatgctg cgccaggggc atgtccccct ctacggcgag ggggacaacc ggatggccct
     5641 catccatgtc gaggatgcag ccgcgctcat cgattttctc agccgcgagg ccccggccgg
     5701 agagagctac aatctcctct gcggggcgcc gcttcgccag gcggaattgg cggagaccct
     5761 gagccggatc agcggtctgc cgatcaaacg cattacccgc cgcgatttcc cttcacgcga
     5821 ccgcggggtg tgggagtcgc tcaccttctc ccagcgcacc gccagccgcc atgccggcct
     5881 ctaccagcgc cacggtttcc gccatccgga tgcgggcgcc gccctcgcct cactctggcg
     5941 ctccttcatc aatactcctt ccgcctgact ttctgatcgt gcagatgctg tccggcagcc
     6001 cc
//
LOCUS       DAOPWU010000207         5961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13268_length_5961_cov_4.217237, whole genome shotgun sequence.
ACCESSION   DAOPWU010000207 DAOPWU010000000
VERSION     DAOPWU010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13268_length_5961_cov_4.217237"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKM23_10435"
     CDS             <1..463
                     /locus_tag="PKM23_10435"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNY91932.1"
                     /translation="VRAICGEYTSLYGNRVTVHTPKTPGTIPYFADFEDSLDVFGWTL
                     NEGTAQTNEWHIGTATNNVAEYRALLCSLELAREMGADHLTVHSDSELLVKQMNGLYR
                     VKNEQLLSLYLQARALCAGFSSVEIAHVPRSRNRRADQLANLALDTPDPKA"
     gene            557..1627
                     /gene="carA"
                     /locus_tag="PKM23_10440"
     CDS             557..1627
                     /gene="carA"
                     /locus_tag="PKM23_10440"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNY91933.1"
                     /translation="MESKKLKARLELEDGSVYHGFSFGAQHTTAGEVVFNTSMVGYPE
                     TLTDPSYHGQILVLTYPLIGNYGVPGSDVVDGIERNFESDRIHVHGLIVSEYSSEYSH
                     WNARRSLGEWLTQNGIPALSGIDTRALTKRLRDEGTMLGRILFFAEEQTFYDPNKTNL
                     VAEVSRREPVEYGEGDKKVILVDCGAKNNILRGLLHLNLRVRCVPWNYDFSGEEYDGV
                     MLSNGPGDPKMCVETIQIIRKVMERSRPIFGICMGNQLLALAAGADTYKLKFGHRSQN
                     QPCVEVGTKRCYITAQNHGFAVDGRTLPAGWEPWFVNANDGTNEGIRHRSRPFMSVQF
                     HPEAAPGPVDTMLLFEDFSRML"
     gene            1627..4812
                     /gene="carB"
                     /locus_tag="PKM23_10445"
     CDS             1627..4812
                     /gene="carB"
                     /locus_tag="PKM23_10445"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HNY91934.1"
                     /translation="MAEKVIILGSSALKIGEAGEFDYSGSQAIKALKEEGIKTILVNP
                     NIATIQTSEHLADEVYFLPVTPYFVEQVIKKEKPDGILLGFGGQTALNCGVELEREGI
                     LRRYGVAVLGTPVEAIMNTEDRDLFTAKLRQIDVKVPRSHAVTSVEEAERVAGEIGYP
                     VMIRIAYALGGLGSGICRSVEEVRERAHKAFSYTRQILVEEYLLGWKEIEFEIVRDQY
                     DNCLSVCNMENFDPMGIHTGESIVVAPSQTLTSSEYHKLRAIAIRTVRHLGIVGECNI
                     QYALDPFSEDYRVIEINARLSRSSALASKATGYPLAAVAAKLALGHSLIDLPNSVTRV
                     TKASFEPAIDYVVVKIPRWDLKKFRQVSKKIGSSMKSVGEIMAIGRKFEEALQKGLRM
                     LEIGFDGVVLNRPFEIGDLREELAEPTDLRIFAIPEAMEQGMDIDEIHALTHIDRWFL
                     HKIANIVAMKERLGEVGEQRCPRDIMLEAKQLGFSDTQIGRALGQGEEEIRKLRKAYG
                     LAPCVKQIDTLAAEYPAQTNYLYLTYNGEEDDVSFNARNSVMVLGSGAYRIGSSVEFD
                     WCCVNSVMTLKQLNYQTIMVNYNPETVSTDYDVCDKLYFDELSFEAVCDIYDKEKPLG
                     IILSMGGQIPNNLALRLHNAGVRVLGTSALSIDSAEDRHKFSRLLDQLHIDQPAWAEL
                     TSIERAIAFAAEVGYPVLVRPSYVLSGAAMSVASNDAELEKFLNKAARISSDHPVVIS
                     KFIENAKEIEIDAVGNEGELLVYAISEHVENAGVHSGDATIVLPPQRTFLETTRRVKK
                     IASRIAAALKINGPFNIQFVAKDNEVKVIECNLRASRSFPFVSKIFDINFIDLATRVI
                     MGHHPPRIDRSAFDLEYVGVKAPQFSYTRLSGADPTVGVEMASTGEVACLGDDFDEAF
                     LLSLLSVGYRLPIRNVLLSTGPIETKVEFLAATRKLQEMGIQFFATGGTARFMAENGI
                     PARQLHWPLSEKKPGVLDYISERKIDLVINIPKNYQEEELTNDYIIRRAAVDYAIPLF
                     TNMQLARRFVEALSRKSLKELQIKSWREYLQ"
     gene            4904..5605
                     /locus_tag="PKM23_10450"
     CDS             4904..5605
                     /locus_tag="PKM23_10450"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNY91935.1"
                     /translation="MNGMKFWMVILLAGLAVCSLWAADEPQGVRTLAVLDFENNSLKD
                     KEEMAPLCKGLADMFITELSKVRQFQLVERANLQKILSEMSLGQTGVIEESAAQEVGK
                     MLGAQNLVLGSFMLMFDGKLRIDARLVEVESGKTLMAEEATGSPDDLAKLVTRLVNRN
                     IESLNVELSGEEKKALRQADNRSFDAALLFARGLDFEEKGDLANARKCYVLALKHNPR
                     FRRARIRLEALKNTN"
     gene            5642..>5961
                     /locus_tag="PKM23_10455"
     CDS             5642..>5961
                     /locus_tag="PKM23_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91936.1"
                     /translation="MVKHPVRYGGALFVLLLVLFQSCAPAQQNLGHELIERGEYARAV
                     RPLKMAIAQDYTDVQSIRDMGIAMYHAGKLKLARGFLRLALSRAPKDMPTLYYLGMVY
                     ELEG"
BASE COUNT         1211 a         1932 c         1713 g         1105 t
ORIGIN      
        1 ggtgcgtgcc atctgtggcg aatacaccag tttgtatggc aatagagtaa cggttcatac
       61 gccgaagacc ccgggtacca ttccgtactt tgcagacttt gaagactctc tggatgtttt
      121 cggatggacg cttaatgaag ggactgccca aaccaatgaa tggcatatcg gcacggcgac
      181 caacaacgtc gccgagtacc gcgccctgct ttgcagcctc gagctggccc gggagatggg
      241 cgccgaccac ctcaccgtgc acagcgacag cgaactgctg gtcaaacaga tgaacggcct
      301 ctaccgcgtc aagaacgagc aactcctttc gctctatctc caggcccgcg ccctctgtgc
      361 aggattctct tccgtggaga tcgcccacgt tccgcgcagc cggaaccgcc gcgccgacca
      421 gctcgccaat ctggcgctcg atacccctga cccgaaagca tgagcggccc cgcggctgcg
      481 cctgcccccg gcggccctgc actgcgactt tttccttatc ccggttattc actgttcaac
      541 aggctggacc tcaacaatgg aatccaaaaa acttaaagca agactggaac tggaagacgg
      601 ctcggtctat cacggcttct ccttcggcgc ccagcacact acagccggcg aggtggtctt
      661 caacaccagt atggtcggtt atccggagac cctcactgat ccctcctatc acggccagat
      721 tctggtcctg acctaccccc tcatcggcaa ttacggcgtg cccggcagcg atgtggtcga
      781 cggcatcgaa cgcaatttcg aatcggaccg catccacgtg cacgggttga tcgtctccga
      841 gtactcttcc gaatacagcc actggaacgc ccggcggagc ctgggcgagt ggctgacgca
      901 gaacggcatt cccgccctct ccgggatcga cacccgcgcc ctgaccaagc ggctgcgcga
      961 cgagggcacc atgctcggcc gcatcctctt tttcgccgag gaacagacct tttacgatcc
     1021 caacaagacc aatctggtcg ccgaggtgag ccggcgtgag ccggtcgagt acggcgaggg
     1081 cgacaagaag gtcattctgg tcgactgcgg cgccaagaac aacattctgc gcggcctgct
     1141 tcatcttaac ctgcgcgtgc gctgcgtgcc ctggaattat gatttcagtg gcgaggagta
     1201 tgacggcgtc atgctctcca atggcccggg cgatcccaag atgtgtgttg agactatcca
     1261 gatcatccgc aaggtgatgg agcgcagccg ccccatcttc gggatttgca tgggcaacca
     1321 gctgctcgcc ctggctgcgg gcgccgacac ctacaaactc aagttcggcc accgcagcca
     1381 gaaccagccc tgcgtcgagg tggggaccaa acgctgttac attaccgcgc agaaccacgg
     1441 cttcgccgtc gacgggcgca ccctgcctgc cggctgggaa ccctggttcg tcaatgccaa
     1501 cgacggcacc aatgagggca tccggcaccg ctccaggccc ttcatgagtg tccaattcca
     1561 tcccgaagcg gccccggggc cggtcgatac catgctgttg ttcgaagact ttagcaggat
     1621 gctctaatgg ctgaaaaagt gatcattctg gggagctccg ccctcaagat cggtgaagcg
     1681 ggcgaatttg actattccgg cagccaggcg atcaaggccc tcaaggaaga gggcatcaag
     1741 accatcctgg tcaatcccaa catcgccacc atccagacct ccgagcatct cgccgacgag
     1801 gtctactttc tgccggtgac cccctatttt gtcgagcagg tgatcaagaa ggaaaagccg
     1861 gatggcatcc tgctcggttt cggcggccag accgccctca actgcggggt cgagctggag
     1921 cgggagggaa ttctgcgccg ctacggggta gctgtgctcg gtaccccggt tgaggcgatc
     1981 atgaacaccg aggaccgcga tctcttcacc gccaaactgc gccagatcga cgtcaaggtg
     2041 ccgcgcagcc acgcggtcac ctcggtcgag gaggcggagc gggtggccgg cgagatcggc
     2101 tacccggtga tgatccgcat cgcctacgcc ctcggcggcc tcggctcggg catctgccgc
     2161 agcgtcgagg aggtgcgcga gcgcgcgcac aaggccttca gctacacccg ccagatcctg
     2221 gtggaggagt atctgctcgg ctggaaggag atcgaattcg agatcgtccg cgaccaatac
     2281 gacaactgcc tttcggtctg caacatggag aatttcgatc ccatggggat ccacaccggc
     2341 gagagcatcg tcgttgcccc gagccagacc ctcaccagca gcgaatacca caaactgcgc
     2401 gcgatcgcca tccgcaccgt gcgccatctc ggcattgtcg gtgaatgcaa catccagtac
     2461 gctctggatc ccttttccga ggattatcgg gtcatcgaga tcaacgcccg gctctcgcgc
     2521 agctcagccc tcgcctccaa ggcgaccggc tatccccttg ccgcggtggc ggccaagctg
     2581 gccctcggcc acagcctcat cgatctgccc aactcagtca cccgcgtcac caaggcgagc
     2641 tttgaacccg ccatcgacta tgtggtggtc aagatcccgc gctgggatct gaaaaagttc
     2701 cgccaggttt cgaaaaaaat tggctcctcg atgaaatcgg tcggcgagat catggccatc
     2761 ggccgcaagt tcgaggaggc cctgcaaaag gggctgcgca tgctcgagat cggcttcgac
     2821 ggggtggtgc tcaaccggcc cttcgaaatc ggcgatctgc gcgaggagct ggccgaaccg
     2881 accgacctgc gcatcttcgc catccctgag gccatggagc aggggatgga tatcgacgag
     2941 atccacgcac tcacccacat cgaccgctgg ttcctccaca agatcgccaa tatcgtcgcc
     3001 atgaaagaac ggctcggcga ggtgggcgaa cagcgctgcc cgcgcgacat catgcttgag
     3061 gccaagcagc tgggcttttc cgacacccag atcggccgcg ctctcgggca gggcgaggag
     3121 gagatccgca agctccgcaa ggcctatggc ctggccccat gcgtcaagca gatcgacacc
     3181 ctcgccgctg aatacccggc ccagaccaac tatctctatc tgacctacaa cggcgaggag
     3241 gacgatgtct ccttcaacgc ccgcaactcg gtgatggtgc tgggcagcgg ggcctaccgc
     3301 atcggcagct ccgtcgaatt cgactggtgc tgtgtcaatt cggtgatgac cctcaagcag
     3361 ctcaactacc agaccatcat ggtcaactac aatcccgaga ccgtcagcac cgactatgac
     3421 gtctgcgaca agctctactt cgatgaactc agcttcgagg cggtctgcga catctacgac
     3481 aaggagaagc cgctcgggat cattctttcc atgggcggac agatcccgaa caaccttgcc
     3541 ctgcgccttc ataacgcggg ggtgcgggtg ctgggcacct cggccctgag catcgacagc
     3601 gccgaggacc gccacaagtt ctcccgcctg ctcgaccagc tccacatcga ccagccggcc
     3661 tgggccgaac tcacctccat agagcgggcg atcgccttcg ccgctgaggt cggctacccg
     3721 gtgctggtgc gcccctccta cgtcctgagc ggggcggcga tgagcgtcgc ttccaatgat
     3781 gccgaactgg aaaaattcct caacaaggcg gccaggatct cctccgacca tccggtagtc
     3841 atttccaaat tcatcgagaa cgccaaggag atcgagatcg acgccgtcgg caacgagggc
     3901 gagctgctgg tctacgccat cagcgagcat gtcgaaaacg ccggggtcca ttccggcgac
     3961 gccaccattg ttctgccgcc gcagcggacc tttctggaga ccacgcggcg ggtcaaaaag
     4021 atcgccagcc gaatcgcagc ggccctcaag atcaacggcc ccttcaacat ccagttcgtc
     4081 gccaaggaca acgaggtcaa ggtgatcgag tgcaacctgc gcgcctcgcg cagctttccc
     4141 tttgtctcca agatcttcga catcaacttt atcgacctgg cgacgcgggt gattatgggc
     4201 catcatcctc cgcgcatcga ccgctcggcc ttcgacctcg agtatgtcgg ggtcaaggct
     4261 ccgcagttct cctacacccg cctcagcggc gccgatccca ccgtaggggt ggagatggcc
     4321 tccaccggcg aggtcgcctg cctgggcgac gatttcgacg aggcctttct gctctccctg
     4381 ctttcggtcg gctaccgcct gcccatccgc aatgtcctgc tctcgaccgg tccgatcgag
     4441 accaaggtgg agtttctcgc cgccacccgc aagctgcagg agatgggcat ccagttcttc
     4501 gccaccggcg gcaccgcgcg tttcatggcg gaaaacggca ttcccgcccg gcagctgcac
     4561 tggccgctct cggagaagaa accgggtgtg ctggattaca tcagcgagcg caagatcgat
     4621 ctggtcatca atatccccaa gaattaccag gaggaggagt tgaccaacga ttatatcatc
     4681 cgccgggcgg cggtcgatta tgccatcccg ctcttcacca acatgcagct ggcgcggcgc
     4741 tttgtcgagg ccctgagccg caaatccctc aaggagctgc agatcaagag ctggcgggag
     4801 tatctgcagt agctgcggcg gccggatcga tcgactaaaa aatcacttgt gcttgtgcat
     4861 aaatttcgat aaaatccaga agatgtccta accggagtac gccatgaatg ggatgaagtt
     4921 ttggatggtg atcctgctcg ccggcctcgc cgtgtgcagc ctgtgggcgg cggatgaacc
     4981 gcagggggtg cgtaccctcg ccgtgctcga tttcgagaac aacagcctca aggacaagga
     5041 ggagatggcg cccctctgca agggcctcgc cgacatgttc atcaccgagt tgagcaaggt
     5101 ccgccagttt cagctggtcg agcgcgccaa tctgcaaaag atcctctcag agatgagcct
     5161 cggccagacc ggggtgatcg aggagtcggc cgcccaggag gtgggcaaga tgctcggggc
     5221 gcagaatctg gtcctcggct ctttcatgct catgttcgac ggcaagcttc gcatcgacgc
     5281 gcgcctggtc gaggtcgaga gcggcaagac cctgatggcc gaggaggcga ccggatctcc
     5341 cgacgatctc gccaagctgg tcacccgact ggtcaaccgc aacatcgaga gcctgaatgt
     5401 cgagctgtcg ggggaggaga aaaaggccct tcggcaggcc gacaaccgtt cctttgacgc
     5461 cgcccttctc ttcgcccgcg gcctggattt cgaggaaaaa ggcgatctgg cgaatgcccg
     5521 caagtgctat gtgcttgcgc tcaagcacaa ccccaggttc cgccgcgccc gcatccggct
     5581 cgaggccttg aagaacacca actgatccgc cagctttttc tgaacgcagt gagaggcaac
     5641 catggtcaaa catcctgttc gttacggcgg tgcgcttttt gttctgttac tcgtcctctt
     5701 ccagagctgc gccccggccc agcagaacct cggtcatgag ctgattgagc gcggggagta
     5761 tgcccgggcg gtgcgtccgc tcaagatggc gatcgcccag gactatacgg acgtccagtc
     5821 catccgcgac atgggcatcg ccatgtacca cgccggcaag ctcaagctcg ctcgcggttt
     5881 tctccgtctt gccctcagcc gtgcccccaa ggatatgccg accctctatt acctcggcat
     5941 ggtctatgaa ctcgaggggc a
//
LOCUS       DAOPWU010000208         5951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13293_length_5951_cov_4.997965, whole genome shotgun sequence.
ACCESSION   DAOPWU010000208 DAOPWU010000000
VERSION     DAOPWU010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13293_length_5951_cov_4.997965"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PKM23_10460"
     CDS             complement(<1..534)
                     /locus_tag="PKM23_10460"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNY91937.1"
                     /translation="MNELLAGIVNLHLGNFVMFGIALILIYLAIAKDYEPMLLLPIGF
                     GALLANIPFSSAIGHEGFLTVLYNFGIRTELFPVLIFIAVGAMIDFKPLLQNPFMLFF
                     GAAAQFGIFACLFVAVLMGYDLKEAASIGIIGAADGPTSIYVANKFAERLLAPISVAA
                     YSYMSLVPIIQPPVIRAL"
     gene            complement(591..965)
                     /locus_tag="PKM23_10465"
     CDS             complement(591..965)
                     /locus_tag="PKM23_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNY91938.1"
                     /translation="MKYVITINSQKYEVEVEKGKATLVEQAPLSVAGASAPAPAAAQP
                     VAVPAAAAAVQGEKLLAPMPGVVISTRVEPGARVKRGQPLLVLEAMKMENEVVSTCEG
                     VVAFLVARGTRVAADDVLAIIG"
     gene            complement(994..1344)
                     /locus_tag="PKM23_10470"
     CDS             complement(994..1344)
                     /locus_tag="PKM23_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91939.1"
                     /translation="MTIKAMLESYEAEIIILFAIVIGAIIAVKVVSAFIAGARQRAAA
                     ASAAARQEILPAGEASPDSRTTALINGYQVSGEGLSLSDVDEKTAAMILAIVAHDAGI
                     PIAELQFKSIRATS"
     gene            complement(1447..2829)
                     /locus_tag="PKM23_10475"
                     /pseudo
     CDS             complement(1447..2829)
                     /locus_tag="PKM23_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin attachment protein"
     gene            complement(3132..5027)
                     /locus_tag="PKM23_10480"
     CDS             complement(3132..5027)
                     /locus_tag="PKM23_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91940.1"
                     /translation="MIKLAVYLLAAGVCISTVYGYENTKAHPAINNAIISFGENYLAD
                     KLPDKNKWRITSDRETYGGTGVTNSGYSYATYSEGTLNLTLLQWVEHGGYSADEPELG
                     AAIRHFYDPTGHTGGRKYLTNRGTSWEWLYTLDNLLHPQTDAIEWAMGDTDKGAYNDW
                     SLKAGKKYLAFAFELSDEAEKRMCLAKAYRCLGEVLHNTADMACPPHVRNDSHAAPLG
                     YAWGELLGSPDPYEELFDPQWAADYLNGKGDPSLESFFASATSIRSINEKLAQFTNHN
                     FFTEETINGAGYQPLSPINKDGDYPQPQLQNLDYDANDFTFYRRFPSGNLVKLCRDKR
                     IFSLRGTPYVDLDCAKSQAAELVPNILCAGANIIRLFLPQFQVQIREAVPNDYIKGQV
                     VHVKSSEYPESILYSGPIDLIDANTGRSIEKLNCSTGEFIGRNPKFKNGDQLIARITL
                     GGFTIESPVYTVTGPVGSTHRIGFQVRVDGHFLNQVATPDTSYSYENDADGATFSSSG
                     DYIGGFASNTFTASYTRTVVHMQTNGTISAVFSSDKLKIISLIWTETTTTQYYNKTVN
                     CTAVNIPLSYSYSGTSIYEIKGEEAKIAITSLTVVQTAAEGLSYSLQSWSTDWNGQVY
                     ASLTKLE"
     gene            complement(5038..5454)
                     /locus_tag="PKM23_10485"
     CDS             complement(5038..5454)
                     /locus_tag="PKM23_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91941.1"
                     /translation="MKTRRIRRSRWPFGILLVSLLIICYTCTNKQSPNSPSDENNLSE
                     TPALTAFSDSLITAFSSENEDDVLALVRSEYRATCDAELNGTRNTMTAVAQALKKRQL
                     LFANALYAEYKVSIDGTEFRIAYSNCGDGHWQLIRF"
     gene            complement(5862..5933)
                     /locus_tag="PKM23_10490"
     tRNA            complement(5862..5933)
                     /locus_tag="PKM23_10490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5899..5901),aa:Ile,seq:cat)
BASE COUNT         1312 a         1560 c         1687 g         1392 t
ORIGIN      
        1 cagcgccctg atcaccgggg gctgaatgat tggcaccagg gacatgtagg aataggcggc
       61 caccgagatc ggcgccagca gccgctcggc gaacttgttg gcgacataga tggaggtcgg
      121 accgtccgca gccccgatga tgccgatcga ggcggcctcc ttgaggtcat aacccatgag
      181 cacggcgacg aaaaggcagg cgaagatgcc gaactgggcg gcggctccga aaaagagcat
      241 gaagggattc tgcagcagcg gcttaaagtc gatcatcgca ccaaccgcga tgaagatgag
      301 gacgggaaaa agctcagtgc gaatgccaaa attatagagc acagtcagaa agccctcatg
      361 gccgatcgcg gaggaaaagg ggatgttggc cagcagggcg ccaaagccga tgggaagcag
      421 cagcatcggc tcgtaatcct tggcgatggc cagatagatg agaatcaggg caatcccgaa
      481 catgacaaaa ttgcccagat gcaggttgac aatgcccgct agcagttcat tcatcactct
      541 tctccactgg aattatcgaa aggcacggag tatttgtccg ccgccaggcc tcagccaatg
      601 atggccagaa catcatccgc ggcgacccgc gtgcctctgg ccaccaggaa ggcgacgacg
      661 ccttcgcagg tggagaccac ctcgttctcc atcttcatcg cctcgaggac cagcaggggc
      721 tgccccctct tcacacgggc tccgggctcc acacgtgtac tgatgacgac gcccggcatt
      781 ggtgcgagca gcttctcccc ctgcactgct gccgctgcag ccggaactgc gacgggctgc
      841 gctgccgctg gcgccggggc tgatgcaccg gcgaccgaca gcggggcctg ctctacgagg
      901 gtcgccttgc ctttctccac ttcgacttca tatttctgac tgttgatggt gatgacatac
      961 ttcatggctt cattccgtcc tttgactccg ctatcagctg gtcgccctga tggatttgaa
     1021 ctgcagttct gcgatgggta tgcctgcgtc atgggccacg atggccagga tcatggccgc
     1081 ggtcttttcg tctacatccg acaatgacaa gccctccccc gagacctggt aaccgttgat
     1141 gagcgcggtg gtacgggagt cggggctggc ctctccagca gggaggatct cctgcctcgc
     1201 agcagccgag gcggcggctg cgcgctgccg ggcgccggcg ataaaggccg agaccacctt
     1261 gaccgcgatg atggctccga tgacaatggc aaacagaata atgatttccg cctcatagga
     1321 ctcgagcatg gccttgatcg tcatgaaggg gcctccctat atcctggaaa tggtgtaatc
     1381 gaccgtgatg gccggccagg ccggcggttc tgcggcgggc gctggggctt cctttttgac
     1441 cccctgttta cgcacggaga ctgttgcttc gcccttgaga aagataatgc ccttcggccc
     1501 gcaggccgcc gcgataaaga tattctcgtc gttcaccggc aggcccgcct cctcgagcat
     1561 ctttctcgcc gccgcgatcc cctttttggg atcgcgttcg ttaatgtcga tcaccatctc
     1621 ggtggttggc tccagaccga gctgccgggc ggcaatgcgg accacctcgg gatcgggcgc
     1681 ggctggcgtc ttgccgaaat agccgagcac catcttgcca tagccctcgg cgatcttttc
     1741 ccacttgccg aagatgacgt tgttgaaggc ctgctggaag tagaactggg aaaccggcgt
     1801 caccgaggtg ccaaagccgc cgagttcgac gacctccccc atcgccctga tcacctccgg
     1861 atagcggttc atgattttgt tgtcgcgcat catctgggtg ttggaggtga gcgctccgcc
     1921 gggcatgggc gaaaagggga tgagcggctc cacctgcttc gcctccgggg gcatgaaata
     1981 gtctttcatg cactccttga agacctcctc ggcctcacgc atgcggtcga tatcgatgcc
     2041 gagatcatag tcagtgccgc gcagggcgtg ccacatcgag atgatgtcgc tctggcaggt
     2101 gccgcccgaa accggcgcca tcgaaaggtc aagacagtcg gcgccgccgt cgagggccgc
     2161 caggtaggcc gccacgcagg ttccagccgt atcatgggag tggaagcgaa ggggtgtaga
     2221 gtcgccgagc agggcgcgcg cttgcttgat ggtctcgtaa acggttctgg gcgtcgaggt
     2281 gcccgaggca tccttaaagc agactgagtc gtaggggatg cccgccgcga ggatcttttt
     2341 cagggtgtcg acatagaaag cgggcgtatg cgcccagtcc gcccccgggg tatcgggcgg
     2401 aaggctcatc atcgtcacca ccacctcgtg tctgaggccc gcttcgtgga tgcactgtcc
     2461 tgaaaaaatg agattgttga catcattgag ggcgtcgaaa ttgcggatgg tggtcacgcc
     2521 gtgttttttg aacatctgcg cgtgcagctt gatgatatcg cgcggctggg aatcgagaca
     2581 gacgacattc acgccgcggg ccagggtttg cagattgact tcgggcccga ccgtcgtccg
     2641 gaacatgtcc atcatgtcga aggaattttc gttcgcataa aaaatggggg actggaacat
     2701 cgctccgccc cccgactcaa agtgatcgat ccccgcctcg gcagtcgcct ttacggccgg
     2761 catgaaatca tccatgaaaa cgcgcgcgcc gtaaacggac tgaaaaccat ccctgaaggc
     2821 tgtgatcatc acatcgattt ttttctttgc caccaggcag cctctccctt tgttgatctg
     2881 ccgaagagtt caggcttccg gcccagggct ggaaggtggt ataaattaac gaactataac
     2941 tataaacaca acctgaattt ttcgcagggc agttggcggg ggtattcata aatagggagg
     3001 cagcgccgtc tgtcaaggcg gccagggagc ggtggcgggc cgaaacagca cagaggggct
     3061 tttcacctga aagcccctgc ataagaacct tcgcaagttg gtctcggtga gattgagttg
     3121 cacaaatttg gtcattccag ctttgtcaaa ctcgcgtaaa cctggccgtt ccagtctgtg
     3181 ctccagctct gcagagaata gctgagtcct tctgcggcgg tttgcaccac ggtaagtgaa
     3241 gtgattgcaa ttttagcctc ttcgcccttg atttcgtaaa tggaggtgcc cgagtacgaa
     3301 taagaaaggg ggatgttgac ggcggtgcag ttgactgttt tgttataata ctgcgtggtg
     3361 gttgtttcgg tccagatgag agagattatc ttaagcttgt cactgctgaa tacagcggag
     3421 attgtgccat ttgtttgcat atggacaacg gttcgtgtat agctggccgt gaaggtattg
     3481 ctggcaaagc cgccgatata gtccccggag gaagaaaagg tagctccgtc ggcgtcgttc
     3541 tcataactgt aagacgtatc cggtgtggct acctgattga ggaaatggcc atcgacacgg
     3601 acctgaaagc caatacgatg ggtgctgcca acggggccag tcaccgtata tacaggcgat
     3661 tctatagtga atcctccgag agtaatgcgt gcgatgagct ggtcgccatt tttaaacttt
     3721 ggatttctgc caataaactc ccctgtggaa caattcagtt tttctatgga tcgcccggta
     3781 ttggcatcga tcagatcaat ggggccggaa taaaggatcg attccggata ctcactgctt
     3841 ttcacatgca cgacttgacc ttttatgtaa tcattcggca cggcctcgcg gatttgcacc
     3901 tggaattgcg gcaagaaaag ccggatgata ttcgcgcctg cgcagaggat attgggcacc
     3961 aactcggcgg cttgcgattt ggcgcaatcc aggtcaacgt agggcgtgcc tcgcagactg
     4021 aaaatgcgtt tatccctgca aagtttcacc aggttaccgc tggggaaacg acggtaaaag
     4081 gtgaaatcat ttgcatcata gtccagattt tgcagttgag gttgagggta atcgccgtct
     4141 ttgttgatcg ggctaagggg ttggtatccc gccccgttga tggtctcttc agtgaaaaaa
     4201 ttgtgattgg tgaattgggc cagtttttca ttgatggagc ggatggaagt ggcgctcgca
     4261 aaaaagcttt caagggaggg gtctcctttg ccatttaaat aatctgcggc ccattgggga
     4321 tcaaagagtt cttcgtaggg atccggactg cccagtaact ccccccatgc atagcccagg
     4381 ggtgctgcat gactgtcgtt gcggacatgg ggcgggcacg ccatgtcagc ggtattgtgc
     4441 agcacctcac ccagacagcg ataggctttg gccagacaca ttcttttctc ggcctcatcg
     4501 ctcagttcaa acgcaaaagc cagatatttc ttccctgctt tcagcgacca atcgttatag
     4561 gctcccttgt cagtgtcgcc catggcccat tcgatggcat cggtttgagg atgcagcaga
     4621 ttgtcgaggg tatataacca ttcccacgaa gtgccgcgat ttgtcagata cttgcggccg
     4681 ccggtatgtc cggtgggatc gtaaaagtga cgaatcgccg cgcccagttc gggttcatcc
     4741 gccgaatacc cgccatgctc gacccattgc agcagggtga ggttcagtgt accttcgcta
     4801 taggtggcat aagagtatcc ggagttggtt acgcctgttc cgccgtatgt ttcacgatcg
     4861 ctcgtgattc gccatttatt tttatctggc aatttatcgg ccagataatt ttcgccgaaa
     4921 gaaatgatcg cattgttgat cgccggatgc gccttggtat tttcatagcc gtatacggtc
     4981 gagatgcaga ctccggcggc gagaagatac acagctagtt ttatcatgac agcatcctta
     5041 aaatctgatt aactgccagt ggccgtcgcc acaattgctg taggcgattc ggaattctgt
     5101 gccatcgatg cttactttat actcagcata caatgcatta gcgaatagaa gctgccgttt
     5161 cttcagcgcc tgggcgactg ctgtcatcgt gttccgggtt ccgttcagct ccgcgtcgca
     5221 agtggcgcga tattccgatc tgactagagc caggacatca tcctcattct ctgagctgaa
     5281 agcggtgatc aggctgtcgg aaaaggctgt tagggcaggt gtctcgctca agttgttttc
     5341 gtcacttgga gagttgggag attgcttgtt cgtgcaggtg tagcagataa tcaggaggga
     5401 taccagcagg atgccaaaag gccatcttga gcgtcgaatg cgtctcgtct tcatgatgaa
     5461 gctccttttc atcaaaaaga aattcccgag gcaggatcgt cgagtcagag caattttgat
     5521 ttaaacaacg ctgatccatt gcatagagca tatctgcttc aaggctgtgc tgataataag
     5581 ggcccaatga gacggcttaa ttttcaattt cttccgaagc ccatcttacc tgcactgtaa
     5641 aaaggagtta aattcaattt attcgtgtga tattttctag tttttgcctc cattctcaat
     5701 acatatttca ggaaagcagg agcgagccag accttcttgg cacacttatt atgaaatgta
     5761 accttcaatg gtatcaaaat caataaaaat cggtaaattc aacgggtaat gatgaacaac
     5821 aaaaaagccc caaactgatc tatggtttga ggctttcaat gtgggcctgg taggtttcga
     5881 acctacaacc tgcggattat gagtccgttg ctctgccgtt gagctacagg cccgtgattc
     5941 ggacgatcga t
//
LOCUS       DAOPWU010000209         5940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13325_length_5940_cov_5.355140, whole genome shotgun sequence.
ACCESSION   DAOPWU010000209 DAOPWU010000000
VERSION     DAOPWU010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13325_length_5940_cov_5.355140"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PKM23_10495"
     CDS             <1..1236
                     /locus_tag="PKM23_10495"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNY91942.1"
                     /translation="LPILEQSDAVGHAVRAGYMYAGIADIAALTGDRAWIRAIDRIWE
                     NVAAKKIHLTGGIGAVGGHEGFGANYELPNLTAYNETCASIANVYWNHRLFLLHGEAR
                     YLDILERTLFNALLSGVAFDGSHFFYPNPLASTGQHERKEWFGCACCPGNITRFLASI
                     PGYLYTVNGDQLYVALYAESSAEIDLQGKALQLKQVCSYPWDGRIQLLVNPPTGGQKF
                     TLNLRIPGWAQGKPLATDLYHYLDPAPAGVKLEINGQSWPLEQKDGFARIRRTWKPGD
                     WVELLLPMPVRRVVAHDSVAADRGLVALERGPLVYCLEWPDVAGGHVHNLLLGDDAPV
                     ESAWNSTLFNGVQTLSATARSYEQDEAGKVTSSEQAVTAIPYYAWAHRGRGGMTVWLA
                     RLESAVHPFGEPRDEKKEL"
     gene            1274..2740
                     /locus_tag="PKM23_10500"
     CDS             1274..2740
                     /locus_tag="PKM23_10500"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNY91943.1"
                     /translation="MRKTILLLFALLIGAAGAQNAPDFDDYFTGGALRLELFQTGDSD
                     EEIISMGQICREPLWPGSRARLIDRFNNGRYEIKVYDIATNLLIYSSGFDCMYGEYRT
                     TTPALEGIKRTFRRAVRIPLPKRPVNFVLEKRDRNNLPAPFFILRIDPADYHINTEAV
                     KTGEIHEAHISGDPAVCADLLFIAEGYTASDKQKFNTDVDRMRDFLFTIAPYSEMKEK
                     LNLRGLFIASPESGMDEPRQGSYKGTLFNASFNAFDLDRYMLVDDGHLLRSIASQAPY
                     DALIILVNSTRYGGGGIYNDYAITTVDHAMSKRVFVHEFGHSFAGLGDEYYTSEVAYN
                     DFYPKGIEPLEPNITALLDPDHIKWAELLTPGVSLPTEYGRRALDSLMSCRRENGVAM
                     EKALQSARSKGASEKELERIKKPYLEKVKKIDAAFTAQRKKNEHLADAVGAFEGAGYS
                     AEGLYRPMIDCLMISNNRDRFCRVCEAAIARMIDHYAR"
     gene            3165..>5940
                     /locus_tag="PKM23_10505"
     CDS             3165..>5940
                     /locus_tag="PKM23_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91944.1"
                     /translation="MKKWLLISLLLTLALPYSLQAVGDAGHSLDFSGATSGNSVIVPY
                     NSVMNNINTSYTIEAWIKWEGTTLPSGSWRIVDRRNYFALYIAPNATYGAFRLWFLAY
                     PSPSGSVAVASTITISNLNAWHHVAVAVQPSGASYAAYLYVDGVQAGSATAAAFGLLN
                     PASTTYPLNIGISWANTGQFNGQIDEVRFWTVRRTQAQIRDNRGVPLDGGTAGMQLYY
                     KFDDGSGTTATESTPNHLHGTVSNPAVPNPTWQVSTAPIGFNLLDPNSGNFSWGSPVD
                     LLWSVNPVVTQVDLHRSSDGGTSWDLIAAGLANPANAIGTATDIVPMVETANALYRVT
                     RAGDASAYDTNDTPITWTGVGFVPTLLEYEAETATLSEAMFTGVDGLAFDCQFIFSAK
                     NTGGGEGSGTITVDVPAPGATYYLWARMMGEGETRNSIFVSADNGPEYVVDVPADERW
                     HWQQAMQSRSGTTPAAFALSAGSHTITIRGRERYCRVDRIALTNDPRPGFYSPEPNQW
                     LQLTRPAELDGPLSIVRNTPFEITWEALNMGPLVNLEYSLDYGRTFTLIDEDVQNTGS
                     FVWNVPDILTEEGVIRISAPLGQCPMDINWMPFYIVNPPPVVTITAPNGGETLTAKET
                     TQITWSSARFDGPVSLYLSLDGGATWSEIAAGQANTGSCTWIVPKTLSDSCLIKVKAA
                     EAEIPVDLSDALFSIVRPAGEEPSFTLIAPNGGESFEAGRVENIEWISEYVTGHAALL
                     FSADSGRTWSEFATGLPAAGSFAWTIPAISSEQCLVKVADATGGAVADLSDAPFSISA
                     VPENFALAFDGEDDLLQIAHHPSLNLSAGMTIAFWMQNHQLGQSWSRILEKGTFDEYA
                     ISFYSNTGRMAGTLRSAIPGGTRMQTLFGPSFSIIPVREWVHVAVTYDGVTACLYING
                     ILEVSRTGKAA"
BASE COUNT         1168 a         2115 c         1543 g         1114 t
ORIGIN      
        1 ctccccatcc tggaacagag tgacgcggtc ggccacgctg tgcgcgccgg ctatatgtac
       61 gccggcatcg ccgacattgc cgccctcacc ggcgaccggg cctggatcag ggccattgat
      121 cgcatctggg agaacgtcgc cgccaaaaag atccacctca ccggaggcat cggagctgtc
      181 ggcgggcacg agggcttcgg tgcaaattat gaactcccca acctgaccgc ctataacgag
      241 acctgcgcct ccatcgccaa tgtctactgg aaccaccgcc tcttcctgct ccacggggag
      301 gcccgctacc tcgacatcct cgagcgcacc ctcttcaacg ccctcctctc cggggtggcc
      361 ttcgacggca gccacttttt ctatcccaac cccctcgcct cgaccggcca gcacgagcgc
      421 aaggagtggt tcggctgcgc ctgctgcccc ggcaacatca cccgctttct cgcctccatc
      481 cccggttatc tctataccgt gaacggagac cagctctacg tcgctcttta tgccgagagc
      541 agcgccgaga tcgacctcca gggcaaagcg ctgcagctca agcaggtctg ctcctacccc
      601 tgggacgggc gcatccagct gctggtcaat cccccgacgg gcggccaaaa attcaccctc
      661 aacctgcgca tccccggatg ggcgcagggc aaaccgctcg cgaccgatct ctaccactat
      721 ctcgatccgg ccccggcggg agtcaagctg gagatcaacg gccagagctg gccgctcgaa
      781 cagaaagatg gattcgcgcg catccgccgc acctggaaac ccggcgattg ggttgagctg
      841 ctcctgccca tgccagtgcg ccgcgtcgtc gcccacgaca gcgtcgccgc cgaccgcggt
      901 ctcgtcgccc tggagcgcgg ccccctggtc tactgcctcg agtggccgga tgtggccggc
      961 ggacatgtgc ataatctcct cctcggagat gacgctccgg tggagagcgc ctggaacagc
     1021 accctgttca acggcgtcca gaccctcagc gccacggcca ggagctacga acaggatgag
     1081 gctgggaagg tcacctccag cgaacaggca gtcaccgcca tcccctacta cgcctgggcc
     1141 catcgcggcc gcggcggaat gaccgtctgg ctggcccgcc tggagagcgc agtgcatccc
     1201 ttcggcgaac cccgggacga aaaaaaagaa ctgtaaacaa acgaaatcct tgtcattcta
     1261 cggagttgat cccatgcgaa aaaccatcct cctcctcttc gcccttctga tcggtgcagc
     1321 cggcgcccag aacgcccctg atttcgatga ctatttcacc ggcggcgccc tccgcctcga
     1381 acttttccag accggtgatt ccgatgagga gatcatctcc atgggccaga tctgccgcga
     1441 gcccctctgg cccggcagca gggcccggct gatcgaccgc ttcaacaacg gccgctacga
     1501 aatcaaggtc tatgacatcg ccaccaatct cctgatctat tcgagcggat tcgattgcat
     1561 gtacggcgaa taccgcacca ccacgccggc ccttgagggg atcaagcgca ccttccgccg
     1621 cgccgtccgc atccccctgc ccaaacgccc ggtcaacttt gtcctggaaa aacgcgaccg
     1681 caacaacctg cccgctcctt tttttattct caggatcgat ccagctgact atcatatcaa
     1741 taccgaagcc gtcaaaacgg gcgaaatcca tgaggcccat atcagcggcg acccggccgt
     1801 ctgcgccgat ctgctcttca tcgccgaagg ctacaccgcc tcggacaaac aaaaattcaa
     1861 caccgatgtc gaccgcatgc gcgattttct cttcaccatc gctccctact cggagatgaa
     1921 ggaaaagctc aatctccgcg gactcttcat cgcctccccg gagagcggca tggatgaacc
     1981 ccgccagggt tcctacaaag gcaccctttt caacgcatct ttcaacgctt tcgacctgga
     2041 ccgctacatg ctcgtcgatg acggtcacct gctgcgcagc atcgccagtc aggcccccta
     2101 cgatgccctc atcatcctgg tcaacagcac ccgctacggc ggaggcggca tttacaacga
     2161 ctatgccatc acaaccgtcg atcacgccat gagcaaacgg gtctttgtcc atgaattcgg
     2221 ccactccttt gccggtctcg gcgacgaata ttacacctcg gaggtggcct ataacgattt
     2281 ctatcccaag ggcattgaac ccctcgagcc caatatcacc gccctgctcg atccggatca
     2341 catcaaatgg gcggagctgc tcacccccgg cgtgagcctg cctaccgagt atggacgcag
     2401 agccctcgac agtctgatgt cctgccgccg cgaaaacggc gtggcgatgg aaaaagcgct
     2461 gcaatcggcg aggtcaaagg gggcctccga aaaggagctg gagaggatta aaaaacccta
     2521 tctggaaaaa gtcaaaaaga tcgacgccgc gttcacagct cagcgcaaaa aaaacgaaca
     2581 cctggccgac gccgtgggag ctttcgaggg agccggttat tccgccgaag gactctaccg
     2641 gccgatgatc gactgcctga tgatcagcaa caaccgggac cgcttttgca gggtctgtga
     2701 ggctgccatc gcgcgcatga tcgaccacta cgcccgttga aattctgccg gcgggcggcc
     2761 tccccaaccc gccggtaccc ccccatccgg ggcggctctc gccacctgct catcagccgc
     2821 cccgcctctc cactcccccg accacacccg ccccctcctt cgcagtcgat cccgctttcg
     2881 ggttcacccg ggaagccggc cgagggtttt tgctattttc tgcttgattt ttaattaaat
     2941 agccttatat tatcatgtac tatccggatt ctcgccgata catgccgtcg gtcactgcac
     3001 ccttgatcgg gcaccctcag ttctgcagcc attctgtaca gcacccccct tattctttca
     3061 tttatcccct cgggctggtt cagcccgacc ggaacggacc ggcttctatc ggatgctttc
     3121 atccctcccg tgggatgcac tcaactctat gcgaaggagc ggttatgaaa aaatggctgc
     3181 tgatctctct gctgctcacc ctggctctgc cgtactccct ccaggccgtc ggtgatgcgg
     3241 ggcacagcct cgattttagc ggggcgactt ccggcaattc ggtcatcgtg ccctacaatt
     3301 ccgtgatgaa taacatcaat accagctata ccatcgaggc ctggatcaag tgggaaggca
     3361 ccaccctgcc ctccggatcg tggcgcattg tcgaccggcg caactatttc gccttgtaca
     3421 ttgcacccaa tgccacctac ggggctttca gattgtggtt cctggcctat ccctcaccct
     3481 caggcagtgt ggcggttgcg agcaccatca ccatttccaa cctcaacgcc tggcaccatg
     3541 tcgccgtggc ggtgcagccc tccggggcca gctacgcagc ctatctctat gttgacggcg
     3601 ttcaggccgg atcggccaca gccgctgcct tcggtctgct caatcccgcc agcaccacct
     3661 atcctctgaa catcggcatc tcctgggcca acaccggaca attcaacggt cagatcgatg
     3721 aagttcgatt ctggaccgtg cgccgtacac aagcccagat ccgcgataac cgcggcgtcc
     3781 ccctggatgg cggcaccgcg ggcatgcagc tctactacaa gttcgatgac ggcagcggca
     3841 ccaccgccac tgaatcaacc cccaaccacc ttcacggcac ggttagcaat ccggccgtac
     3901 ccaacccgac ctggcaggtc tccactgccc cgatcggctt caacctcctc gatcccaaca
     3961 gcggcaactt tagctgggga agcccggtcg atcttttgtg gtcggtcaat cccgtcgtca
     4021 cccaggtcga tctgcatcgc agcagcgacg gcgggacgag ctgggatttg atcgccgccg
     4081 gtttggccaa tcccgccaat gccataggca ccgccaccga catcgtcccc atggtggaga
     4141 ccgccaacgc cctctatcgc gtcaccaggg cgggcgacgc cagcgcttat gacaccaatg
     4201 acaccccgat cacctggacc ggagtcggct tcgtccccac cctgcttgaa tatgaggcgg
     4261 agaccgccac cctcagcgag gcgatgttca ccggcgttga cggtctggct tttgactgcc
     4321 agttcatctt ctccgccaag aacaccggcg gcggcgaggg cagcggcacg atcactgtgg
     4381 acgtccccgc gcccggcgcc acctactacc tctgggcacg catgatgggc gagggtgaaa
     4441 ccagaaactc gatctttgtc agcgccgata atggtccgga gtatgttgtc gatgtgcccg
     4501 ccgatgaacg ctggcattgg cagcaggcca tgcagtcgcg cagcggcaca acgccggccg
     4561 ccttcgccct ctccgccggc agccacacca tcaccattcg cggccgcgag cgctactgcc
     4621 gcgtcgaccg cattgccctc accaacgatc cgcgtcctgg cttttacagc cccgagccca
     4681 atcaatggct ccagctcacc cggcccgcgg agctggatgg accgctctcc atcgtccgca
     4741 acacgccctt cgaaattacc tgggaagccc tcaacatggg ccccctggtc aacctcgaat
     4801 actctctgga ttacggccgc accttcaccc ttatcgatga agatgtgcag aacacgggct
     4861 ccttcgtctg gaacgtaccc gatatcctca cggaggaggg tgtcatccgc atctccgcgc
     4921 ccctcggcca atgcccgatg gatatcaact ggatgccctt ctacatcgtc aatcccccgc
     4981 cggttgtgac catcaccgca cccaatggcg gggagaccct gacagccaag gagaccaccc
     5041 aaatcacctg gagcagcgcc cgcttcgacg ggccggtctc tctctatctc agcctcgatg
     5101 gcggcgccac ctggagtgaa atcgccgccg gccaggccaa taccggcagc tgcacctgga
     5161 ttgttccaaa aaccctctct gactcctgcc tgatcaaggt caaggctgcg gaggcggaga
     5221 ttccggtcga cctcagcgat gccctcttca gcatcgtccg ccccgccggt gaagaaccct
     5281 ccttcaccct gatcgcgccc aacggaggcg agagctttga ggccggccgc gtcgaaaaca
     5341 tcgagtggat ctcggaatat gtcaccggcc atgccgcact gctcttctcc gccgacagcg
     5401 gacgaacctg gagcgagttc gccaccggcc tgcctgcagc gggctctttc gcgtggacca
     5461 tccccgccat ctcctcggag cagtgcctgg tcaaggtggc cgatgccacc ggcggcgccg
     5521 tggcggatct ctccgatgcc cccttctcca tctcggctgt ccctgagaat tttgccctgg
     5581 ctttcgacgg cgaggatgat ctgctccaga tcgcccatca ccccagcctg aacctctccg
     5641 caggcatgac tatcgccttc tggatgcaaa accatcagct gggacagagc tggagccgca
     5701 tcctcgaaaa gggcaccttc gacgaatacg ccatctcctt ctacagtaac accggccgca
     5761 tggccggcac cctgcgcagc gccatccccg gcggcacgag gatgcagacc cttttcggcc
     5821 cctccttctc catcatcccc gtgcgggaat gggtccatgt cgccgtgacc tatgacggcg
     5881 tcaccgcctg cctctacatc aacggcatcc ttgaggtgag ccggaccggc aaggccgcac
//
LOCUS       DAOPWU010000210         5886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13491_length_5886_cov_4.894701, whole genome shotgun sequence.
ACCESSION   DAOPWU010000210 DAOPWU010000000
VERSION     DAOPWU010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13491_length_5886_cov_4.894701"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PKM23_10510"
     CDS             complement(<1..195)
                     /locus_tag="PKM23_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNY91945.1"
                     /translation="MSGWLTTNRPDLNFEDTSVGRMKKRIWEASPEEIDRILQEYEIP
                     SPSELGKPGTYIQNTIRHRVV"
     gene            complement(211..1395)
                     /gene="iolM"
                     /locus_tag="PKM23_10515"
     CDS             complement(211..1395)
                     /gene="iolM"
                     /locus_tag="PKM23_10515"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="scyllo-inosose 3-dehydrogenase"
                     /protein_id="HNY91946.1"
                     /translation="MKAVTLFADWKPRPGFQLGAKDIEGKLSYLGSKVWCNPRIEMVE
                     KEVPVPGPTEVLIEVKACGICGSDVHMLQSDEQGYIFYPGLTAFPCTLGHEFSGVVAE
                     AGPEAFNKRTGKRFLPGEAVCAEEMMWCAQCRPCADGLPNHCERLQEIGFSIDGAYAR
                     YIRMDARYLWSIEAFRPIYGEEKMFLLGSLVEPSSVAYNAVIVRGGGVLPGESVLIMG
                     SGPVGIAACAELRRAGAAKVIISEPSRSRREMALALGATHAIDPAAGDVPGAVLDITE
                     GMGVKIILEATGLPSVVWPDIEKIIWEGRQLNATVVLVARADDRIIMNGEVFQVRRAN
                     VVGAQGHSGHGIFPNVINSMADGMDMTPLITKKITLEETAEHIKLLQTNREQVKITVL
                     PR"
     gene            complement(1507..2520)
                     /locus_tag="PKM23_10520"
     CDS             complement(1507..2520)
                     /locus_tag="PKM23_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY91947.1"
                     /translation="MKKKINVGLVGVGRLGKMYAGFLAQRVPQARVVAYADIIPERAS
                     TAAEEYGEARWYDNHHDLNNDPEVEAVIVTATTQSHKEIIIDAAAAGKAVFSEKPLTL
                     NLADAREIERAVEKAGIFFQMGYMRRFDKGFAAAKQKIDEGVVGIPVTFRGSSRDPYR
                     PSLDYLKPENSGGQILDMAIHDIDIARWYMGEVATVYSMGTVLAYPEIKEVNDTDNVI
                     MALRFESGALGEIDISRNGVYGYDIRAEVLGSQGTVMAGYLRDTPIQVMTKAGVTHDV
                     VPYFPERFGEAYVTQLNDFLTNLSAGLEPMIKLEDGIKALQIGTAATWSLHHDQIARV
                     KDF"
     gene            complement(2547..3686)
                     /locus_tag="PKM23_10525"
     CDS             complement(2547..3686)
                     /locus_tag="PKM23_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNY91948.1"
                     /translation="MSAPAGPIFEMPVIIDRNSPVPQYFQLQTWLIGQIDGGFFKPND
                     KIPTEEELARQTGMARATIRQAIQNLVNMGYLYRKRRFGTFVLSQTAAAAGRNRIVGV
                     LVHDIRSGYAPEFLRGIGDEAARHNYSVILCNTDDLYSRAEFHASQLIRQEINGLIFM
                     PTAAPAEKNLQLIETFHQKNIPVVLFDREIPEHDHDIVTTDNFQGAYDLTAYLIRMGH
                     RRIAITLSTSFSSERSRLAGYKQAMLDHSLVVDPGLLFTLEERFIDKHYQQYARIILA
                     QRHHYTALFAGNDPIAYILYNAATEMGIAVPAELSLAGYDDLAFSNSHPMELTTVHQP
                     IYEMGQESIKLLLRRIEGEGGEPQRVVLKSYLVERRSVQALGLQS"
     gene            3908..4818
                     /locus_tag="PKM23_10530"
                     /pseudo
     CDS             3908..4818
                     /locus_tag="PKM23_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
     gene            complement(4893..>5886)
                     /locus_tag="PKM23_10535"
     CDS             complement(4893..>5886)
                     /locus_tag="PKM23_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91949.1"
                     /translation="PFVTPYGQLLEVDALLDTTAVKSAAWLQFVQKSGPFTLTAGGRF
                     DYFNLIDQGSAFSPRLALEYALDPITKLSASVGRYHQAPAYVWLVANPENRSLRHIQV
                     DQFILGLQRYLKEDVKISLEGYYKDYDRYPVSTLQPWLVMVNTGAGFGGAEEGFASFG
                     LDPLVSEGSGWARGVELFLQKRIAEVPHYALLSVSYNESKFRGLDGVLRPSSFDQRWI
                     VNFGGGYIPNNRWEFSAKFRLATGRPYTPYNADYSRSAESYNTARIAVNHSLDLRADR
                     RWSLPGVDLITYVDIQNIYNRAYRDVPRWDTFRQELDERASIGILPSIGISAEF"
BASE COUNT         1076 a         1781 c         1872 g         1157 t
ORIGIN      
        1 caccacccgg tggcggatgg tgttctggat ataggtgccg ggcttgccca gctccgaggg
       61 tgaaggaatc tcatactcct gcaggatgcg gtcgatctcc tcgggagagg cctcccagat
      121 ccgttttttc atgcggccga ccgaggtgtc ttcgaagttc aggtcgggcc ggttggtcgt
      181 cagccatcca ctcatggtac tatcctttcg ctatcttggc aacaccgtga ttttcacctg
      241 ctcgcggttg gtctgcagca gcttgatatg ctctgccgtc tcctccaggg tgatcttttt
      301 ggtgatcaat ggggtcatgt ccatgccgtc ggccatggag ttgatgacat tcgggaagat
      361 gccgtggccg gagtggccct gggctccgac gacgttggcg cgccgcacct gaaagacctc
      421 gccgttcatg atgatgcggt cgtcagcgcg cgccaccagc accacggtgg cgtttagctg
      481 tcgcccctcc cagatgatct tttcgatgtc gggccagacc acactcggca ggccggtcgc
      541 ctcgaggatg atcttgacgc ccatcccctc ggtgatgtcg agcaccgcgc cagggacgtc
      601 gcccgcggcc gggtcgatgg cgtgagtggc gccgagggcc agggccatct cccgccgcga
      661 cctggagggc tcggagatga tcaccttggc cgcccccgcc cgccgcagtt ctgcacaggc
      721 ggcgatcccg accggcccgc tgcccatgat caacacgctt tcgcccggca gcaccccgcc
      781 gccgcgcacg atgaccgcgt tgtaggcgac cgaggagggc tcgaccaggc tgccgagcag
      841 aaacatcttt tcctcgccgt agatcggccg gaaggcctcg atgctccaga gatagcgggc
      901 atccattcgg atgtatctgg cataggcgcc gtcgatcgaa aaaccgatct cctgcagccg
      961 ctcgcagtgg ttgggcaggc cgtcggcgca gggccggcac tgcgcacacc acatcatctc
     1021 ctccgcgcag accgcctcgc cgggcagaaa acgtttgcct gtccgcttgt taaaggcctc
     1081 gggaccggcc tcggccacca cgccggaaaa ctcgtggccg agggtgcagg gaaaggcagt
     1141 cagcccggga taaaagatgt agccctgctc gtccgactgc agcatgtgga cgtcgctgcc
     1201 gcagatgccg caggccttga cctcgatcag cacctccgtc ggtccgggca ccggcacctc
     1261 cttctccacc atctcgatgc gggggttgca ccagaccttg ctgccaagat agctcagctt
     1321 gccctcgata tccttggctc ccaattgaaa gcccggcctg ggtttccagt cggcaaaaag
     1381 cgtgacggct ttcatacctt ttcctttcca tgataaaacc gggcggacaa gatcaaccgg
     1441 cccgccggct ggtacaattc actgcaatgg gtgcactccg gtattatcgc gaccccggcg
     1501 ggcgccctag aaatccttca cccgggcgat ctggtcatgg tgcagcgacc aggtcgccgc
     1561 cgtgcctatc tgcagggcct tgatgccatc ctccagcttg atcatgggct cgagccccgc
     1621 gctgaggttg gttaaaaaat cattgagctg ggtgacatac gcctcgccga aacgctccgg
     1681 gaagtaggga accacatcgt gggtcacccc cgccttggtc atcacctgga tgggggtgtc
     1741 acgcaggtag ccggccatca ccgtcccttg tgagcccagc acctcggcgc ggatgtcata
     1801 gccatagacc ccgttgcggc tgatgtcgat ctcgcccagg gcgccgctct cgaagcgcag
     1861 ggccatgatg acattgtcgg tatcgttgac ctctttgatc tcgggatagg ccagcaccgt
     1921 gcccatggaa tagacggtgg cgacttcacc catgtaccag cgggcgatgt cgatgtcgtg
     1981 gatggccata tcgaggatct ggccgccgct gttctcgggt ttgagatagt cgaggctggg
     2041 ccggtaggga tcgcgcgatg agccgcggaa ggtgaccgga atgccaacga cgccttcatc
     2101 gattttctgt ttggcggccg caaagccctt gtcgaaacgg cgcatgtagc ccatctggaa
     2161 aaaaatcccc gctttttcca cggcgcgttc gatctcgcgg gcgtccgcaa ggttgagggt
     2221 gaggggcttt tcactgaaca ccgccttgcc ggccgccgcc gcatcgatga tgatctcctt
     2281 gtggctctgg gtggtggcgg tcacgatcac cgcctccacc tccggatcgt tattaaggtc
     2341 gtgatggttg tcgtaccacc tggcctcccc atactcctcc gcggccgtgg aggcacgctc
     2401 ggggatgata tccgcataag ccaccacccg cgcctgcggc acgcgctggg cgagaaatcc
     2461 ggcgtacatc tttcccaggc ggccgacgcc aaccaggccg acattgattt tctttttcat
     2521 ggcttccttc gtgaaatggt taatcgtcaa gactgcaggc cgagggcttg cacagaccgc
     2581 cgctcaacca gataggattt caataccacc cgctgcggct cacccccctc accctcgatg
     2641 cggcggagca gcagcttgat gctctcctgc cccatctcgt agatgggctg atgcactgtc
     2701 gtcagctcca tcgggtgaga gttggagaag gcgaggtcgt cgtagcccgc cagggagagc
     2761 tcggcgggta cggcaatgcc catctccgta gcggcattat agagaatata ggcgatagga
     2821 tcgttgccgg cgaagagggc ggtatagtga tggcgctgag ccagaatgat gcgcgcatac
     2881 tgctgataat gtttgtctat gaaacgctcc tcgagggtga agaggaggcc gggatcgacc
     2941 acaagactgt ggtcgagcat ggcctgcttg tagccggcga ggcgggaccg ttcgctgctg
     3001 aaggaggtgc tcagagtgat ggcgatgcgc cggtggccca tgcggataag ataggcagtc
     3061 aggtcataag ccccctgaaa attatcggtg gtaacgatgt cgtggtcatg ctccgggatc
     3121 tcgcggtcga acaggaccac cgggatgttc ttctgatgaa aggtctcgat gagctgcagg
     3181 ttcttctccg ccggcgccgc tgtgggcatg aagatcaggc cgttgatctc ctggcggatg
     3241 agctggctgg cgtggaactc ggcgcggctg tagaggtcat cggtgttgca gagaatgacg
     3301 ctgtagttat ggcgcgcggc ctcgtcaccg atgccgcgca gaaactcggg ggcgtagccg
     3361 ctgcggatgt cgtgcaccag caccccgacg atacggttcc tgcccgccgc tgccgccgtc
     3421 tgcgagagga caaaggtgcc aaaccggcgc ttgcggtaaa ggtagcccat gttgaccaga
     3481 ttctggatgg cctggcggat ggtggcgcgg gccatgccgg tctggcgggc gagctcctct
     3541 tcggtgggaa tcttgtcgtt gggcttgaaa aagcccccgt cgatctgacc gatcagccag
     3601 gtctgcagct ggaagtattg cggcacagga ctgttgcggt cgatgatcac aggcatctca
     3661 aagatggggc cggccggtgc gctcaacagt gcaggagttt cttgccccgg tcagtggggt
     3721 ttgggccaac ttcgattcaa ttgtctatac aattatacaa taaaaaggaa tttgagtcaa
     3781 ggcttttttg cccagcgctg catttttttc ttgttccctc acctggaatt ctgtatactt
     3841 gatcaatata gccggcggcg gcaggcagga cagctggagc ggaacacaca agaggggaga
     3901 gcgaacgatg aaaatagcca tcacgggggc aactggcctg gtgggcgcgg gattggtccg
     3961 ccatctgacc cggcagggcc acgagatcca tcccctggtg cggcggccgc ctctggcagc
     4021 ggcgcacgaa atcttctggg atccccagaa ggatgaaatc gatcatgctg cactggaggg
     4081 aatggatgcc gtcatccatc tcgccggtga aaatctctcc ggctactgga gcgcggccaa
     4141 gaaggaacgc atcatggcca gccgcagtcg cgggacccgc ctgctcagcc aggccctggc
     4201 cgtcctccat cgcaaaccgg ggctcctcat cagcgcctcg gcgatcggct tttacggcga
     4261 tcgcggcgcg gaggcgctgg acgaattctc ggcacgcggc gagggttttc tggctgacgt
     4321 ctgcatggag tgggagaacg cagccgaccc ggcccgcgag gcgggcatcc gcgtcctcca
     4381 cagccgcttc ggcatcatcc tcgaccctgg tggaggggcc ctggccaggc tgctgcccct
     4441 cctgcacctg ggactggggg gcaggctcgg cagcggacgt cagtactgga gctggatcac
     4501 catgcaggac gtccacagcg ccctggcctt ctgcctggat catcccgacc tcgaaggcgc
     4561 ggtcaatttc accgcccccg gcgcggtcac caatgccgaa tttacacggg ctgcagcgcg
     4621 attcctcacc gccccgcttt tttcgctgtg ccggccttcg ccctgcgcct ggtgatgggc
     4681 gagatggccc aggagatgct cctcagcagc acgcgggccg agcccaaaaa actcctcgcc
     4741 agcggattcc gcttcgatca caccgatctc cgcgccgcct tcgccgccgt tctcggcccc
     4801 aacctgccag cccgttaaca aagacagcgg cggatgcaat aaaaaagccc ggtttgcacc
     4861 gggctttttc atttcaagct gagttgagga ggctagaatt cggcgctgat gccgatcgag
     4921 ggcaggatgc cgatgctggc gcgctcgtcc agctcctgcc tgaaggtgtc ccagcgcggg
     4981 acgtcgcgat aggcccggtt gtagatgttt tgaatgtcaa cataggtgat cagatccacg
     5041 ccggggaggc tccagcgccg gtcggcgcga aggtcgagcg agtggttgac cgcgatacgg
     5101 gcggtattgt aactctccgc cgaacggctg taatcggcgt tgtagggggt ataggggcgg
     5161 ccggtggcga ggcggaactt ggcgctgaac tcccaccggt tattggggat atagccgccg
     5221 ccgaaattga caatccagcg ctggtcgaag ctgctcgggc gcagcacccc gtccagtccc
     5281 ctgaatttgg actcgttata gctgacgctg agcagggcgt agtggggcac ctcggcgatg
     5341 cgtttttgca gaaagagctc gactccgcgc gcccaaccgc tcccctcgct gaccagggga
     5401 tcgaggccga aggaggcaaa gccctcctcg gcgccgccga atcccgctcc ggtgttgacc
     5461 atcaccagcc agggctggag ggtgctgacc ggatagcggt cgtaatcctt gtaataaccc
     5521 tcgaggctga ttttgacatc ctccttgaga tagcgctgga ggccgaggat gaactggtcg
     5581 acctggatgt ggcgcaggct gcggttctcc gggttggcga ccagccagac ataggcgggg
     5641 gcctgatggt agcgcccaac actggcgctc aatttggtga tcggatccag ggcatattcg
     5701 agggcaaggc gcggcgagaa ggcactgccc tggtcgatga gattgaaata gtcaaagcga
     5761 ccgccggcgg tgagagtaaa ggggccgctc ttctggacga actgcagcca ggcggcgctc
     5821 ttgacggcgg tggtgtcgag cagggcatcc acttcgagca gctggccata gggggtgacg
     5881 aagggg
//
LOCUS       DAOPWU010000211         5860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13562_length_5860_cov_5.229630, whole genome shotgun sequence.
ACCESSION   DAOPWU010000211 DAOPWU010000000
VERSION     DAOPWU010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13562_length_5860_cov_5.229630"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..912)
                     /locus_tag="PKM23_10540"
     CDS             complement(94..912)
                     /locus_tag="PKM23_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91950.1"
                     /translation="MAFSDLDGLYELVKSRRLDSARIHSVRDLTRIHLDPHYSLIVAV
                     DSDGGIGPMQADTVFCPAYELGRFAIRVPLLEILCSGAIPLAAFDMLTMKMAGVGAEI
                     VHGIRDELKLAGYGDEFHLSGSTEDNVPTKMTGVGTTIIGVVKERDFRPGSSQPDDMV
                     VCLGLPKSAPEDTVRTDDPEIIQQSDLRVLLGIAGVHDILPVGSHGIGYEAQAMARDA
                     GLRFVADAGCRLDMKKSAGPSTCVLASCRDDAAGLLSAQINAPVILAGKMSTIN"
     gene            complement(924..1676)
                     /locus_tag="PKM23_10545"
     CDS             complement(924..1676)
                     /locus_tag="PKM23_10545"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNY91951.1"
                     /translation="MNEFFNNLDKSSIIPFYYQLQEVLENLIEQGVYKPDEKLPSENE
                     LKEWLGISRTTAQRALKHLVDRGLAKRIQGKGTFVANKTITYSLVAAFSFSTEMLGLN
                     KKVRSKLICAEEIQAHSLISKMLQTAADTRFYSIQRIRYVDNEPISLQTSYLPVVLVP
                     GLIEKNFQEESLFETIKKEYGLIIGDAQETLQAVKATEYEARHLNIREGDAVFLLERI
                     TRITTGEILEFVKTILRGDTGKFYIELSSEPK"
     gene            complement(1688..3058)
                     /locus_tag="PKM23_10550"
     CDS             complement(1688..3058)
                     /locus_tag="PKM23_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNY91952.1"
                     /translation="MARQVFFPEWLHRCRLFFELLVLPCWLLSCDTPVRVGLIGDQFG
                     AVNADSSFAVMDRAVQRLMSEQPDLLVHVGDMVESARNIRSFEDYRKNWLTATAIMDK
                     PDRPWFLTFGDHDVVPPRYEPMSSDDSREQWALSLAHLQGLPVDSLPWYGVSIKGYHF
                     IFLYSLENLHTDPRWGSIFLNSISDHQMEWLKAELESHKKAKGIIVILHHPHWYVWSN
                     WYRVHELLRRYPVRAVVAGHFHYAQDEGVLDGIRYFIMGSSGGVINNTDPNSGGAFVY
                     GLMTLSGKRAPAFLLREVYSDSVIAVPSRRSMDRIQAIQCMLDDLWQDENIVLRQGKL
                     FGKSGDRLLPLRSIGLESISNPIDLPIRISIGYDTTLIGAARWNAGPAQGDGGAELEL
                     SPGLGAGWANYSNVGKWDKSKPVWVAELLPQVTYHKPSIAFNISVRFKDDRVRQVRRT
                     IEYHIN"
     gene            3706..4980
                     /locus_tag="PKM23_10555"
     CDS             3706..4980
                     /locus_tag="PKM23_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNY91953.1"
                     /translation="MKVLNENPLVSILLPLLFLMPLLAWSQTASSLPFSVYPLYDESG
                     EPDKCGFDPEQAASLRKSLGDHWGYGYDDLLADLPVWRSSPYVTIDSIGASVQNRAIW
                     ELAITADPVPARPRRTIHIHARTHPGEVQSWWVTREIIKILLAEDDFSRSVRDSCVFC
                     IIPMYNPDGVELEYPRENANGIDIESNWNKYPLQPEVAALRKRFYELMTSGAPIEIAL
                     NMHSAYGTSRYFVYHAAAGSSDEYAMLQKQFIEGTRAHFPGGIRPWDYFVSWKTGTAT
                     QYPESWFWMNYHESVMALTYEDMNDPSAGEFDRTAHAIVHGILDYLGRSKTQVAASPL
                     PAPAAFVLEQNYPNPFNAGTTISWQLPGDGPVSVRLYDLLGRERAVLFEGIQTAGRHA
                     LRWDASGWPSGVYFIGMQAGRYRAIRQAVIAR"
     gene            complement(5056..5805)
                     /locus_tag="PKM23_10560"
     CDS             complement(5056..5805)
                     /locus_tag="PKM23_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005574067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HNY91954.1"
                     /translation="MHSAEFRFYEELNDFLPPERRKQSFLYAFAGTPAVKDAIEAQGV
                     PHTEVDLILVNGLSVGFDYHLQPGDRVAVYPVFESLDIFPVSRVRPEPLRRIRFIADV
                     HLGKLARWLRLLGFDTTYRNNLADADIIAIALELNRIILTRDLGLLKNKAVTHGYWVR
                     ASIPGEQTREVIQRFTLLRSAAPFSRCLECNGGIEAIARAEAHKSVPPQVAAACDSFY
                     RCQSCGRIYWQGSHHARLTERLKSLGLEPGA"
BASE COUNT         1278 a         1661 c         1654 g         1267 t
ORIGIN      
        1 gaggagggca tgcccatcgc atcgccggcg gcgacaccga ccagacaccc cttgatttgt
       61 ttcttgatcc tttccgactg catttattcg gcctcagtta atggtgctca tctttcccgc
      121 caggatgaca ggagcgttta tttgggcgga taaaagtcct gctgcgtcgt ctcggcagga
      181 ggcgaggacg caggtggagg ggcccgccga ctttttcata tccaaccggc agccggcatc
      241 cgcgacgaac ctcaagccgg catccctggc catggcctgc gcctcataac cgatgccatg
      301 gctgccgacc ggcaaaatat catggacgcc ggcgatcccc agcagaaccc tgagatcact
      361 ttgctggatg atctccggat cgtctgtccg gacggtgtcc tcgggtgccg acttgggcaa
      421 gcccaggcag acgaccatgt catccggctg cgaggagccc ggccggaaat ccctctcctt
      481 gaccactccg atgatggtcg tgccgacacc ggtcatcttc gtcggcacat tatcctccgt
      541 gctgcccgaa agatgaaact cgtcgccata gccggccagt ttcagctcat cgcggatgcc
      601 gtggacaatt tcagcgccga cacccgccat cttcatggtc agcatgtcaa atgcggccag
      661 gggaatcgca ccggaacaga ggatttccag cagcggcacg cgaatggcga accgtcccag
      721 ttcataggcc gggcaaaata ccgtatccgc ctgcatcggc ccgatgcccc cgtccgagtc
      781 gacggcgacg atcagggagt agtgcgggtc aaggtggatt ctggtcaaat cccgcacaga
      841 atgaattcgg gctgaatcca gccgtcggga ttttacaagc tcgtacaagc cgtccagatc
      901 agaaaaggcc atagcagctc ctgtcacttg ggttccgatg acaattcgat ataaaatttt
      961 ccggtatcac cccggagaat agtcttgacg aactcgagga tttcacctgt tgtgatcctg
     1021 gtgatccgtt ccagcagaaa gacggcatcg ccctccctga tgttcaggtg tctggcttca
     1081 tattcggtgg cctttaccgc ctgcagggtc tcctgagcat caccgatgat gaggccatac
     1141 tcctttttga tcgtctcgaa aagcgattct tcctgaaagt tcttttcgat caggcccgga
     1201 accagcacca ccggcagata ggaggtttgc agcgagatcg gttcgttgtc tacatagcgg
     1261 atgcgctgga tgctgtaaaa cctggtgtcg gcggccgtct gcagcatctt ggaaatgagg
     1321 ctgtgcgcct gaatctcctc tgcgcagatc agtttgctgc ggaccttttt attcaggccg
     1381 agcatctctg tggagaaact gaatgccgca accagactgt aggtgatggt cttgttggcg
     1441 acaaaggtgc ccttgccctg aattctcttc gccagaccgc ggtcgaccag gtgtttcaag
     1501 gcgcgctggg cggttgtacg gctgatgccc agccactcct tcaattcatt ctccgagggc
     1561 agcttttcat ccggtttata gacaccctgc tcgatgagat tttccagcac ttcctgcagc
     1621 tgatagtaaa atggaatgat gcttgatttg tccaggttat tgaaaaattc gttcatggta
     1681 taatacttca gttgatgtga tattcaatcg tccggcgtac ctgacgcact ctgtcatcct
     1741 tgaaacggac cgagatgtta aaagcaatgg acggtttgtg atacgttacc tgaggcagaa
     1801 gttcagccac ccaaaccggt ttgctcttgt cccatttgcc gacattggag taattggccc
     1861 agccggcgcc gagcccgggg ctcagttcca gctctgcacc gccatcccct tgcgccggcc
     1921 ctgcattcca gcgggcggcg ccgatcaggg tggtatcata acctatcgag atccggatgg
     1981 gaagatcgat cggattggag atcgattcca gtccgatcga ccgcagcggc agcagcctgt
     2041 caccgctttt accaaagagc ttgccctgcc gcaagacgat gttttcatcc tgccagagat
     2101 catccagcat acattgaatg gcctggatcc ggtccatgct tcttctggag ggcaccgcaa
     2161 tgacgctgtc cgagtagact tcccggagga gaaacgccgg agctcttttc ccggagaggg
     2221 tcatcagtcc atacacaaag gcgccgccgc tgttgggatc ggtgttgttg atcacgcccc
     2281 cgctggaacc catgataaag tagcggatgc catccagaac gccctcatcc tgcgcatagt
     2341 gaaaatggcc ggcaacaacc gccctgaccg gatagcggcg cagcagttca tgaacgcggt
     2401 accagttgga ccagacatac cagtgcggat ggtggaggat aacgatgatt cctttagcct
     2461 ttttatggct ctccagttca gctttcagcc attccatctg gtggtccgag atcgaattca
     2521 ggaaaatgga gccccagcgg ggatcagtgt gcaaattctc aaggctgtaa agaaaaatga
     2581 aatggtaccc cttgatggag accccgtacc agggcagcga atcgaccgga aggccctgga
     2641 gatgcgccag gctgagggcc cactgctcgc gcgaatcatc actggacatg ggttcatacc
     2701 tgggcggcac aacgtcatga tcaccaaagg tcaggaacca gggccggtcc ggtttgtcca
     2761 tgatcgcggt cgcagtcagc cagttcttgc ggtaatcctc gaaagagcgg atattgcggg
     2821 cactctccac catatccccg acatgaacca gcagatccgg ctgttcgctc atcaatcgct
     2881 gcaccgcacg gtccatcacg gcgaaggagg aatcggcatt gacagcgccg aactggtcgc
     2941 cgatcaggcc gaccctgacc ggcgtatcgc aagagaggag ccagcagggc aggaccagca
     3001 gttcaaaaaa cagacgacac ctgtgcagcc attcggggaa gaaaacttgt ctggccatct
     3061 tcatctcttg tgcatttgac tggttgtccg tacaactatg gcacagggtt cagaaaaaca
     3121 tgtattataa catgaaaaaa gttttaaatc gttgtgcaga cctcagcaag agtgggttgg
     3181 gaagggataa ccgttagaac agagccgtca gatggttgtg gagaacagat caatcaggaa
     3241 gggcaacagc cgcatggtga tattcctgat cctctcacag tataacgtaa aatttgcaaa
     3301 tgtgcaagag gaaagtgaaa ttcctgaaaa ttattcagcc ccccactctg gcgccgaccc
     3361 ggcgggaaac agacgaaacc ggggcacact gcgagcatct tgctgagcca ggcccgcttg
     3421 acttgcaacg ggttcccttc tgcacatgcg gcggcaaggt gaaaccgttt ggtcccttca
     3481 ggccggagca ggctgtcact ggttttcatt tgcattcgcg gctgtaaagt tgtactattc
     3541 cgttaccgga tcccaggccg ctctcttcag cacctccacc ctctcgacag gcgcaccgga
     3601 ggtgattctg actgccgcgc cgtcactcct cctgagaagc ggctggacga gcggccccac
     3661 agacggtcaa gtattggcaa attcctttca gcgttggagt tgttcgtgaa agtactgaat
     3721 gagaacccat tggtttcgat cctcctcccc ctcctctttc tcatgcctct tcttgcctgg
     3781 tcccaaacgg cttcctcctt gcctttttct gtttatccat tgtatgatga atccggtgag
     3841 ccggataagt gcggattcga tcctgaacaa gcggcaagcc tgcgcaagag cctgggagat
     3901 cattggggct atggctatga cgatctgctc gccgatctgc cggtctggcg gagcagcccc
     3961 tatgtgacca tcgattccat cggcgccagt gtccagaatc gcgccatctg ggagctggcc
     4021 atcaccgcgg acccggttcc ggccaggccg cgcaggacga tccatatcca tgcgcgcacc
     4081 catcccggcg aggtccagtc ctggtgggtg acccgggaga tcatcaaaat cctgctggcg
     4141 gaggatgatt tttcccgttc tgtgcgcgac tcttgcgtct tttgcatcat cccgatgtac
     4201 aacccggatg gcgtggaact ggagtatccg cgtgaaaacg ccaacggcat cgatatcgag
     4261 agcaactgga acaaatatcc tctccagcca gaggtcgccg cgctgcgaaa acgcttttat
     4321 gaattgatga cctccggtgc gcccatcgag atcgccctga acatgcattc ggcctatggg
     4381 accagccgct attttgtcta tcatgctgcg gccggatcgt cggacgaata tgccatgctg
     4441 caaaaacaat tcatcgaagg cacgcgcgcc catttccctg gcggcatccg cccctgggac
     4501 tattttgtga gctggaaaac cggcaccgcg acccagtatc ccgagagctg gttctggatg
     4561 aattatcacg agagcgtgat ggcgttgacc tacgaggata tgaacgatcc ctcggccggc
     4621 gaattcgatc gcacggccca tgcgatcgtg catggcatcc tcgattatct gggccggagc
     4681 aaaacgcagg tcgctgcctc ccccctgccg gcgccggccg cttttgttct ggagcaaaat
     4741 tatcccaatc ccttcaatgc aggcacgacc ataagctggc agctgcccgg cgatggtccg
     4801 gtcagcgtgc gtctttacga ccttctgggc cgggaaagag cggtgttatt tgaagggatt
     4861 cagacggccg gccggcatgc cctgaggtgg gatgcgtcgg gatggccttc cggcgtctat
     4921 tttattggta tgcaggcagg gaggtatcgg gcaataaggc aggcggtgat tgcgcgttaa
     4981 gaccggatga tagcgcagga tataatggca tcttcttcgg ttctgacctt gaaatagaga
     5041 cgatacacct gacgattatg cgccgggttc cagccccagg gatttgaggc gctcggtcag
     5101 ccgggcgtgg tggctgccct gccagtagat gcggccgcag gactggcatc gataaaagct
     5161 atcgcaggca gcggccacct gcggcggcac agacttgtgc gcctctgcgc gtgcaatggc
     5221 ctcgatcccg ccattgcatt cgaggcaacg gctgaaagga gcggcactgc ggaggagggt
     5281 gaagcgctga atcacctctc gcgtctgttc accgggaata gaggcccgca cccaatagcc
     5341 gtgggtgacc gccttgttct tgagaaggcc caggtcgcga gtcaggatga tgcggttcag
     5401 ctcgagcgcg atggcgatga tgtccgcatc ggcgaggtta ttgcggtagg tggtatcaaa
     5461 gcccagcagc cgcagccagc gggccagttt gccgagatgg acatcggcga tgaagcggat
     5521 gcggcgcagc ggttccgggc gtacccggct gacgggaaag atgtcgaggc tttcgaatac
     5581 cggatagaca gccacgcggt cgcccggctg gaggtgataa tcaaaaccga cggagaggcc
     5641 attgaccaga atcaggtcga cctccgtgtg cggcacaccc tgcgcctcga tggcgtcttt
     5701 caccgccggc gtcccggcaa aggcatagag aaaactctgc ttgcgccgtt cgggcggcaa
     5761 aaaatcattc aattcttcat aaaaacggaa ctcggcagaa tgcatggccg caaaattcct
     5821 ggctgcatgt ataattcaag tcaatgacga cgcctgtaaa
//
LOCUS       DAOPWU010000212         5856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13571_length_5856_cov_4.610929, whole genome shotgun sequence.
ACCESSION   DAOPWU010000212 DAOPWU010000000
VERSION     DAOPWU010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13571_length_5856_cov_4.610929"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="PKM23_10565"
     CDS             complement(<1..1697)
                     /locus_tag="PKM23_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91955.1"
                     /translation="MRRIVSLLFVSLLLLLLAVPGVAQYGKQYEGPDDPAGDKEAERT
                     GIMNGNNVLTFFRNTTEQSDCCNLGYWVSRWPNDYSGSKMHDGICCLFGARVYVENDS
                     IPVTDLNQIRTRSDLDTLFFCQGSYREYMDTSPDGNVEWGLYPVFGYFNVSEDNPNPA
                     MSNKPASWPAAGWPARGDELKWPGTWNGRFGLGIMKADLESYFVANDAQDQEYLGPED
                     RVKYYPRPGRFIGDKGPMPSVQNGLPWGGIGLRVEVRGFQWKNPEAADCIFWEYTIAN
                     VSNYDLPEMYFGYHIDMAVGGEESEPADDIAYYNQDLDMAFTWDLDGQMVGGGGVPGC
                     MGIAFLESPGVSTDGKDNDGDGLTDEQRDNVATRKIGPTEGISNIADFTAFYGSDDDG
                     DGTLSFDFKEHWDADEDQDWRDGDDTNNDGIYQKNEFPGDDVGTDGSGPFDLDYPGPD
                     ANGTECNHMPDLIEGLGSEPNFGFTDVGESDMLGLTSFRYNMNWSIYLLRNDQAEWDF
                     LTQGIFDEAQTTPTNFCEQFATGVFTLYRGRTERISMSELHAMERLHEQSAPPYDAPA
                     "
     gene            complement(1724..2782)
                     /locus_tag="PKM23_10570"
     CDS             complement(1724..2782)
                     /locus_tag="PKM23_10570"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY91956.1"
                     /translation="MRIRNILTVVAFCLLTAGLLFAQTDSRVGTTAANFLELGFGGAG
                     NAMGDAYVSMGRDLNSVYWNPAGLGYLERSQVILNFQPWILDINTTAISAAFANDRFG
                     TVALTLYQTSYGEEKVTNTDMQTGTGETFDGQDLAVNLSYGRKLAQWFSFGATAKYVT
                     SRIWHERAGAVALDLGAIVNTPFFSWTDKPGDGLNIGMSICNYGTKMAYDGIDLKETV
                     DIDPDENGNYAYIPTRFETEGWELPLVARIGLSIHPLVVGSHRVTLAVDFLHANNNSE
                     SMNVGAQYSMAIPAVGEFYLRGGYKGAFMTDSHYGLTAGGGMRLDYLRNQSMMIDYAF
                     RDVDLLGGMHTYTLSFLF"
     gene            complement(2803..5556)
                     /locus_tag="PKM23_10575"
     CDS             complement(2803..5556)
                     /locus_tag="PKM23_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91957.1"
                     /translation="MGFRKSPLLLLFLLICTTAWAQTTGKITGRVTDATKGEPLYGAN
                     VILEGTNLGAVSDFNGDFYIINVAPGRYTMLVRMVGYESARVEAQVSTNRTFDVPIRL
                     HETVIQGQEVVVTASKISIKKDQTSSIKNVSSDQIQLMPVQEVSQVIELQAGVVGGHF
                     RGGRSNEVSYLIDGMQVDETLYGEGSTIQLEKEVVQDLEVITGTFNAEYGKAMSGIVN
                     MVTKDGADQFHGSISANLGNYLPKSDGVYVGLKRGDVTRIQDYKAQIEGPIWKGKINF
                     LATVRYQHVSGFLNGIRRFNVDNYSNMAESNVAGADITPWDVFVNGVRYYSEHTGDSS
                     IVPMSESENYSFFGKLSFNLIKNVRMAATYTLEKGNGQGYSHGYKYNPDGYGKSHSRS
                     AMYSFKLNHAISRSAFHELKFSFTDNWNGYFMYKDPFDPRYLSTQYGGSVGGFSAGGQ
                     DRSHSEYFTQLMTTKYDFTWQATKHHSLKAGVQYSQQYLKNWPVPTRDVKWDSPLLQA
                     RWYDPVAQRMQFFPYQAEFVPEESISMDRYIKQPWDYSLYLQDKMEYESLVMNFGLRY
                     DYFNSNTYYPSNRRNPSNQGLYEDPNMQSTMLKAPIQTQLSPRFGLSYTLGSTAVLHF
                     SYGHFFQMPPMYSLYTNYRFIVPTNDFGTTHGNPLMEAQKTVKYEMGLWQQLLPGLGM
                     DVSVYYSDIYDLQSAVVWTTYNETRYGVYDNKDYGNTKGLELKFDYQSGPLFANLNYT
                     LQYTRGNADNPSSTYSRLAVNIDPVPKLIPMSWDQRHTLNSTLGYTMRNLSVSVTGSF
                     NSGFPYDYNPIPTSRLARQSLLPNNGKKPANYNFDLQGQYDLPLNGNMKLRFFAYVRN
                     LLDTRNEMSVYGYTGHAYESIIYPAQYLSFRSNFNTIEDQYQNPAMYSAPREVKLGVG
                     FMF"
BASE COUNT         1182 a         1523 c         1888 g         1263 t
ORIGIN      
        1 gccggtgcat catagggcgg ggcgctctgt tcatgcaggc gttccatggc atgcagctcg
       61 gacatcgaga tgcgttcagt gcggccgcgg tagagggtga agacgccggt ggcgaactgc
      121 tcacaaaagt tggtcggagt agtctgggcc tcgtcgaaaa taccctgggt cagaaagtcc
      181 cattcggcct ggtcattgcg cagcagatag atcgaccagt tcatgttgta gcggaagctg
      241 gtcaagccga gcatatccga ttcgccgacg tcggtaaagc cgaagttggg ctcgctgccc
      301 aacccctcga tgaggtccgg catatggttg cattcggtgc cgttggcatc ggggccggga
      361 tagtcgagat caaaggggcc ggaaccgtcg gtgccgacat cgtcgcccgg gaattcgttc
      421 ttctggtaga tgccgtcgtt attggtatca tcgccatcgc gccaatcctg gtcctcgtcg
      481 gcgtcccaat gttccttaaa gtcgaaggag agggtgccgt cgccatcgtc atcgctgcca
      541 tagaaggcgg tgaaatcggc gatattgctg atgccttcgg tcggcccgat cttgcgggtg
      601 gcgacgttgt cgcgctgttc atcggttaga ccgtcgccgt cattatcctt gccatcggtg
      661 gagacgccgg ggctttcgag gaaggcgatg cccatgcagc cgggcacgcc gccgccgccg
      721 accatctggc cgtcgaggtc ccaggtgaag gccatatcga gatcctggtt gtagtaggcg
      781 atgtcgtcgg caggttcgga ttcctcaccg ccgacggcca tatcgatgtg atatccaaaa
      841 tacatctcgg gcaggtcata attggagaca ttggcaatgg tatattccca gaagatgcag
      901 tcggcggcct cgggattctt ccactggaag ccgcggacct cgacgcgcaa accgatgccg
      961 ccccagggca ggccgttctg gacagagggc atgggcccct tgtcgccgat gaagcggccc
     1021 ggacggggat aatatttgac cctgtcttcc ggaccgaggt actcctggtc ctgggcatcg
     1081 ttggcgacga aatagctctc gaggtcggcc ttcatgatac cgaggccgaa gcggccgttc
     1141 caggtgccgg gccatttgag ctcgtcgccg cgcgccggcc agccggcggc gggccaggag
     1201 gcgggcttgt tgctcatggc cggattgggg ttgtcttccg agacgttaaa atatccgaaa
     1261 acaggataga gaccccactc gacattgccg tccgggctgg tgtccatata ttcgcggtag
     1321 ctgccctggc agaaaaagag ggtgtcaaga tcgctgcggg tgcggatctg attcaggtcc
     1381 gtcaccggaa tcgaatcatt ttcgacatag acccgcgctc cgaaaaggca gcagatgccg
     1441 tcgtgcatct tggagccgct gtagtcgttg ggccagcggg aaacccagta gccgagattg
     1501 cagcagtccg attgttcggt ggtgttgcgg aaaaaggtga gcacgttatt tccgttcatg
     1561 atgccggttc tttcggcttc cttatcgcca gccgggtcat cggggccctc atactgcttg
     1621 ccgtactggg ccacgcccgg cacagccagg agcagcagca agagtgatac aaaaagcaga
     1681 gatacgatcc gtctcatgcc attctctcca cgatagtgag cagttaaaag agaaaactca
     1741 aggtataggt gtgcatgccg ccgagaagat cgacatccct gaaggcgtaa tcgatcatca
     1801 tgctctggtt gcgcagatag tcgaggcgca tgccaccgcc ggcggtgagc ccatagtgcg
     1861 aatcggtcat gaaggccccc ttgtagccgc cgcgcagata aaattcaccc accgcgggga
     1921 tggccatgct gtactgggcg ccgacattca tggactcgga gttgttgttg gcatggagga
     1981 aatccacggc cagggtgacg cgatggctgc cgaccaccag gggatgaatc gagaggccga
     2041 tgcgggccac caggggcagc tcccagccct cggtttcgaa acgggtcggg atgtaggcgt
     2101 agttgccgtt ctcatccgga tcgatatcga cggtctcctt gaggtcgatg ccatcgtagg
     2161 ccatcttggt cccatagttg caaatgctca tgccgatgtt gaggccatcg ccgggcttgt
     2221 ccgtccagga gaagaagggg gtattgacaa tggcgcccag atccagggcg acggcgccgg
     2281 cgcgctcatg ccagatgcgc gaggtgacat atttggcagt ggccccgaag gagaaccact
     2341 gcgccagctt gcgcccgtaa ctgagattga ccgcaagatc ctgaccgtca aaggtctccc
     2401 cggtgccggt ctgcatgtcc gtgttggtca ccttctcctc gccataggag gtctggtaga
     2461 gggtcaaggc cacggtgccg aatcggtcat tggcgaaggc ggcgctgatc gctgtggtgt
     2521 tgatgtcgag tatccagggc tggaaattga ggatgacctg gctgcgctcc aaatagccca
     2581 ggccggccgg attccagtag accgaattga ggtcgcgccc catactcaca taggcgtccc
     2641 ccatggcatt gcccgccccg ccgaatccca gttcgagaaa attggccgct gtcgtgccga
     2701 cgcgcgaatc ggtctgggcg aataaaaggc cggcggtgag gaggcaaaac gcgacaactg
     2761 tcaaaatatt tcggatgcgc atatccattc tccgcataaa ggttagaaca tgaacccgac
     2821 gcccagcttg acctcgcgcg gggcggagta catcgccgga ttttgatact ggtcttcgat
     2881 agtgttaaag ttggaacgga acgagaggta ctgggccggg taaatgatcg actcataggc
     2941 atggcccgtg taaccgtaaa cactcatctc gttgcgggta tcgagaaggt tgcgcacgta
     3001 ggcgaagaag cgcagcttca tgttgccgtt gagaggcaga tcatactggc cctgcaggtc
     3061 aaagttgtaa ttggccggtt tcttgccatt gttgggcagc aggctctgac gcgccagacg
     3121 gctggtcggg atcggattat agtcgtaggg gaagccggaa ttaaaagagc cagtcacact
     3181 caccgaaaga ttgcgcatgg tgtagcccag ggtgctgttg agggtgtggc gctgatccca
     3241 gctcatcggg ataagcttgg gcaccggatc gatgttaaca gccaggcggc tgtaggtgga
     3301 gctgggattg tccgcattgc cgcgggtgta ctgcagggtg taattgagat tagcgaaaag
     3361 cgggccggac tgatagtcaa atttcagttc cagccccttg gtgttgccat aatccttgtt
     3421 gtcgtagacc ccgtaacgcg tctcattata ggtggtccaa accaccgcac tctgcaggtc
     3481 atagatgtcg ctgtaataga ccgagacatc catgccgagt ccgggcagca gctgctgcca
     3541 caatcccatc tcatacttga ccgtcttctg ggcttccatg aggggattgc cgtgagtggt
     3601 gccgaaatca ttggtcggca cgatgaaacg gtaattggta tagagcgaat acatcggcgg
     3661 catctggaaa aaatggccgt aactgaaatg gagcaccgcc gtgctgccga gggtgtagga
     3721 gaggccaaag cgcgggctga gctgggtctg gataggggcc ttgagcatgg tggactgcat
     3781 attgggatct tcgtagagcc cctgattgga ggggttgcgc cggttggagg gataataggt
     3841 attggagttg aaatagtcgt agcgcaagcc aaaattcatc accaggctct cgtattccat
     3901 cttgtcctgc agataaagcg agtaatccca gggctgcttg atgtagcggt ccatcgagat
     3961 cgactcttcg ggcacaaact ccgcctgata ggggaaaaac tgcatgcgct gggcgacggg
     4021 gtcgtaccag cgcgcctgca gcaggggcga gtcccatttg acatcgcgtg taggcaccgg
     4081 ccagtttttc agatactgct ggctgtactg gacgccggcc ttgaggctgt ggtgcttggt
     4141 cgcctgccag gtaaagtcat atttggtggt catcaactgg gtgaagtatt cggaatggct
     4201 gcggtcctgg ccgccggcgg agaagccccc gacgctgccg ccgtactggg tgctgaggta
     4261 gcgcggatcg aagggatcct tgtacataaa atagccgttc cagttgtcgg tgaaagaaaa
     4321 cttgagttcg tggaaggcgc tgcggcttat ggcgtggttg agcttgaagg agtacatcgc
     4381 ggagcgggag tggctcttgc catagccatc aggattgtac ttgtaaccgt ggctgtagcc
     4441 ctggccgttg cccttttcta gggtgtaggt ggcggccatg cgcacgttct tgatcaggtt
     4501 gaaagagagt ttgccgaaga aggaatagtt ctcactctcg ctcatcggca cgatcgagct
     4561 gtcgccggtg tgttccgagt agtagcggac gccgttgaca aagacgtccc agggggttat
     4621 atccgccccg gcgacattgc tctcggccat gttgctgtag ttgtcgacat taaagcggcg
     4681 gatgccattg agaaaaccgg atacatgctg gtagcgcaca gtggcgagga agttaatctt
     4741 gcccttccag atcgggccct cgatctgggc cttgtagtcc tggatgcggg tgacatcgcc
     4801 gcgcttgagg ccgacataga cgccgtcgga tttgggcaga tagttaccga ggttggcgct
     4861 gatcgagccg tggaactggt cggcgccgtc cttggtcacc atgttgacga tgccgctcat
     4921 cgccttgccg tactcggcgt tgaaggtgcc ggtgatgacc tcgaggtcct gcaccacctc
     4981 tttttcgagc tgaatggtgg agccttcacc gtagagggtt tcgtcgacct gcatgccgtc
     5041 gatgagatag gagacctcgt tggagcggcc gccgcggaag tggccgccga cgacgccggc
     5101 ctggagttcg atgacctgtg agacctcctg gacgggcatg agctggatct gatcggagga
     5161 gacattcttg atcgagctgg tctggtcctt tttaatcgag atcttggagg cggtgaccac
     5221 cacttcctgg ccctggatga cggtctcatg gaggcggatg gggacgtcga aggtgcggtt
     5281 ggtggagacc tgggcttcta cgcgggccga ctcgtagccg accatgcgca cgagcatggt
     5341 gtatcgaccc ggggccacat tgatgatgta aaagtcgccg ttgaaatcgc tgacggcgcc
     5401 gaggttggtg ccttcgagga tgacgttggc gccgtagagg ggctcaccct tggtggcgtc
     5461 cgtgacccgg ccggtgattt tgccggtggt ctgcgcccag gcggtggtgc agatgagcag
     5521 gaagagcagg agcagggggg atttgcggaa acccataacc ttacctcaca ttcaggtgaa
     5581 caaggagtgg gaactggata ttttcgcggt tgtggaatat cctccgggcg gggggcattc
     5641 cgttcggggc ggcatcggtc gcagctgcgg cggggtgcgc ggcccgcaaa aggtgcgcct
     5701 cggtgcggcg gcaaggcgcc gcggcggatg cactttgttt tttttgtata aaaacttact
     5761 ggcgataagc taaggatatt ttagtcaaaa atcaacagga aaatcgccta ttttgggcag
     5821 gttacccccg aaatccggat cccatcttga ctcctg
//
LOCUS       DAOPWU010000213         5844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13618_length_5844_cov_4.950769, whole genome shotgun sequence.
ACCESSION   DAOPWU010000213 DAOPWU010000000
VERSION     DAOPWU010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13618_length_5844_cov_4.950769"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PKM23_10580"
     CDS             <1..1237
                     /locus_tag="PKM23_10580"
                     /inference="COORDINATES: protein motif:HMM:NF036809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNY91958.1"
                     /translation="QISWYAWGWRDQYPADVRARYERDMHHLFDEVLPAEIGAVDPER
                     YYHPGSPATGYLDIPYAEGDAHYWGVWHNRHPFSDYDKNVGRFMSEYGFQSYPERATV
                     EGFSLPGDRELHSPVMLAHQRCMADERRDKEYGNRLIRHYMENDYRLPKDFNSYLYLS
                     QVLQAEGVKRAFHAHRGAMPYCMGSLYWQIDDCWPVASWSSIDYHGRWKALHYFIREA
                     CKTFLLVPVEAEGVLQVKVVSDSLSNTAAELELVVMDFSGRKGFTRTLPVAIRANSSQ
                     LCCSLPAAELLAGFERSSALLRLTLRVGERLLASDIHYFAPVKSLTLPPCTLDAGVTA
                     APGGCVITLRSSSLAKNLCLECSDPEGFFSDNYFDLLPGEGRTIHYRTRLTPAEVKAG
                     LRWQTIHDTYQEEKGKEQE"
     gene            1234..3060
                     /locus_tag="PKM23_10585"
     CDS             1234..3060
                     /locus_tag="PKM23_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HNY91959.1"
                     /translation="MKLTWIDFAIILLYMLGVTAIGLAFKKRAKEGTGSYLLGGNKLP
                     WYLLGLSNASGMFDISGTMWLVTIGFVYGLKSIWLPWLWPVFNQIFLMVFLSAWLRRS
                     NVTTGAQWIGTRFGHDRGATLSHTIVVVFALIGCLGFLAYGFIGLGKFIMIFIPWEIV
                     SPYIPFDVPAQYVPHVYGIVFTLVAVFYTILGGMTSIVLADLLQYIIMTVASIAIAVI
                     AMSALSGSTLNVPDGWMSPFFGWKLDLDWSSIIAQVNDKIASDQFGLFTIIVMLMVFK
                     GILASAAGPAPNYDMQKILATKSPKEAAKMSGFVSLILQPIRYLMIAGFVVLGLLYYD
                     RLNLMVGGKIDFEQILPSAISEFVPVGLLGLLLAGLLAAFTSTFSGTLNAAQAYIIND
                     LYLKYIRPHTTQKQTTMINYTSGLIVVVISMVLGFFAQDVNSLLQWIVSGLYGSYVAA
                     NVLKWYWWRFNSYGYFWGMLAGLIPALIFPLVFKSTLDLYYFPVLLLISIAGCYLGSW
                     LTKPTDEKTLMSFYKTVRPWGFWKPVYEKVKAADPSFERNRNFSRDAVNVLVGTIWQT
                     ALVIIPFYIVLMKKGPFFITLAIIFVTSWFLKKNWLDKLTEE"
     gene            3097..>5844
                     /locus_tag="PKM23_10590"
     CDS             3097..>5844
                     /locus_tag="PKM23_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HNY91960.1"
                     /translation="MHLVPVKEFLMRRRFALVALAFLLPADLLAQAIAPTIARRSENA
                     NRIGLYEKYELQLELGNVVYANPYNPEEIDVRALFTSPGGRQWTIYGFYDHYQNRNEW
                     KVRFAPNEAGTWSYTLMVTTPAGNGSSPAYTFEAAASAHHGWLHTAKANPHYLVHDDG
                     TPFYGVAIFWPWGIAEPGLNTLQSLGCNWVGLWNITYDTGNTLIESMSSGLGRYDQNK
                     CKRIDDILEWLEKRDMALSLSIWPHDLFCKSLSGWAQRWNDNPYKQLCDVLEIYENET
                     AWRYMEKQYRYIIARWGYSRAMGPWEIINEISGTDAWAAGRTAAADEWTRKSLTFLRA
                     HDPFDRPVTASQHGGLFWTAGYGIFDLPNMHLYETDGGTARYASNPVRSSVWAYHEAA
                     RRMWQGYAKPAILGEAGGGSNKNFGGYSPGSAEYTRMFHNALWASWSSGLAASPMWWS
                     FNTHQSDSRFQEQLKAFSLVAPGIDYALRPFQPAEVTVAGADAFAMEDGEMAFGWARE
                     EWGRDLSMRSLQIAGLPDSVYSVVYYDTWKGTAVASHIRPVVEGLLIDELPQIAASAP
                     DLAFIIRPAPTGSVPFRLGLMASPRRLYSDGESYAEVRCLIFDVEGRFCSEAGSILTF
                     NLNGLGRLVGPAVVAAEKGLAMITVQSDSAGSGEAQIMVASPGLIGDTLSISMTDLQL
                     IDDFESYDPANPVGNFWKTTTGTFAALMVEPRPGMAGGQRLRVDYGIGGGKPPYTGFI
                     YTLPAALKGAKFLRFYLQGDGSGRGLAVRINRTSSSYWQYEMALTSTEESMVELPLRG
                     FLPSDGSSPLDLTRALSLSFHILRGSGGDGSGTIALDDILFANSSTSGIAGERGAILP
                     EALQLLQNWPNPFNASTEFSFVLPRRMHPQLSVYDIQGRRVATLAVGERPAGRHRLVW
                     QA"
BASE COUNT         1067 a         1855 c         1690 g         1232 t
ORIGIN      
        1 ccagatctcc tggtatgcct ggggctggcg ggatcagtat cccgccgatg tgcgcgcccg
       61 ctatgaaagg gatatgcacc atctcttcga cgaggtgctt ccggccgaaa ttggtgctgt
      121 cgatccggaa cgctactacc atcctggcag ccctgccacc ggctatctgg atatccccta
      181 tgccgagggc gatgcccact actggggggt ctggcacaac cgccatccct tcagcgatta
      241 cgacaagaat gtcggccggt tcatgagcga atacggtttt cagtcctacc cggaacgcgc
      301 cacagtcgag ggcttttccc ttcccggcga ccgggagctg cactccccgg tcatgttggc
      361 ccaccagcgc tgcatggccg atgaacgccg ggacaaggag tacggcaacc gcctgatccg
      421 ccactatatg gagaacgact accgcctgcc gaaggatttc aacagttacc tctatctcag
      481 tcaggtgctg caggccgagg gagtaaagcg cgcctttcat gcccaccgcg gcgccatgcc
      541 ctattgcatg ggctcactct actggcagat cgatgactgc tggccggtgg cctcctggtc
      601 gagcatcgat taccacggcc gctggaaggc cctgcactac ttcatccgcg aggcctgcaa
      661 gacctttctg ctcgtccccg tcgaggcgga gggcgtgctg caggtgaagg tggtctcgga
      721 ctccttgagc aatacagccg cagagctgga attggtggtg atggatttct cgggccggaa
      781 aggctttacg cgcacgctgc cggtggccat cagggccaac agcagtcaac tctgctgcag
      841 tctgcccgcg gcggaactgc tggcagggtt tgaacgcagc agcgcactgc ttcggctgac
      901 actgagggtg ggggagaggc tgctggcctc ggacatccac tatttcgccc cggtcaaatc
      961 cctgacactg ccgccgtgca cactcgatgc cggggtgacg gcagcccctg gcggctgcgt
     1021 gatcaccctc cgcagcagct ccctggccaa aaacctctgc ctggagtgca gcgatcctga
     1081 gggctttttc tccgataact atttcgatct cctgcccggc gagggcagaa ccatccatta
     1141 ccgcacccgg ctcacacccg cagaggtgaa agccgggctg cgctggcaga caatccacga
     1201 tacttatcag gaagagaagg gaaaggagca ggaatgaagc tgacctggat cgattttgcc
     1261 atcatcctcc tttacatgct gggtgtgacc gccatcggcc tggccttcaa aaaacgcgcg
     1321 aaggagggga ccggttccta tctgctcggg ggcaacaaac tcccatggta tttgctcggc
     1381 ctctccaacg cctcgggcat gttcgacatc tccggcacca tgtggctggt gaccatcggt
     1441 ttcgtctatg gtctcaagag catctggctg ccctggctct ggccggtctt caaccagatc
     1501 ttcctcatgg ttttcctctc cgcctggctg cgccgctcca atgtcactac cggcgcgcag
     1561 tggattggca cccgcttcgg ccatgaccgc ggcgctaccc tctcgcacac catcgtggtc
     1621 gtcttcgccc tcatcggctg tctcggtttt ctcgcctatg gttttatcgg tctcggcaag
     1681 ttcatcatga tcttcatccc ctgggagatc gtttcgccct atatcccctt cgatgtaccg
     1741 gcgcagtatg tgccgcatgt ctacggcatc gttttcaccc tggtggcggt cttttacacc
     1801 atcctcggcg gcatgaccag catcgtgctg gctgacctgc tccagtacat catcatgacc
     1861 gtggcgagta tcgccatcgc ggtgattgcc atgtccgctc tcagtggctc aaccctcaat
     1921 gtgccggatg gctggatgag tcccttcttc gggtggaagc ttgaccttga ttggagcagc
     1981 atcatcgccc aggtcaatga caagatcgcc agcgaccagt tcggcctctt caccatcatc
     2041 gtcatgctca tggtcttcaa ggggatcctc gccagcgccg ccgggccggc gcccaattat
     2101 gacatgcaga agattctggc gaccaaatct cccaaggaag cggccaagat gagcggattt
     2161 gtctccctca tccttcagcc gattcgttac ctgatgatcg ccggctttgt cgtgctcggt
     2221 ctgctctact acgaccgcct caacctgatg gtgggcggca agatcgattt cgagcagatt
     2281 ctgccttcgg cgatcagcga attcgtcccg gtcggtctgc tcggtctgct gcttgccggc
     2341 ctgcttgccg cctttacctc gaccttcagc ggcaccctca acgccgccca ggcctatatc
     2401 atcaacgatc tctatctcaa atacatccgg ccccatacca cgcagaaaca aaccacgatg
     2461 atcaactata cctccggcct gatcgtggtg gtgatcagca tggtgctcgg tttcttcgcc
     2521 caggatgtca acagcctgct gcagtggatt gtttcggggc tctacggcag ttacgtcgcc
     2581 gctaacgtgc tcaaatggta ctggtggcgt tttaacagct acggctactt ctggggcatg
     2641 ctcgcgggtc ttatcccggc cctgatcttc ccgctggtct tcaagagcac cctcgacctc
     2701 tattatttcc cggttctgct cctcatctcc atcgccggct gttatctcgg ttcctggctc
     2761 accaaaccca ccgatgaaaa gaccctgatg tcgttctaca aaacagtgcg gccatgggga
     2821 ttctggaagc cggtttatga aaaggtcaag gctgcggatc cctccttcga gcgcaaccgt
     2881 aatttcagcc gcgatgcggt caatgttctg gtcggcacca tctggcagac ggcactggtg
     2941 atcatcccct tctatattgt cttgatgaaa aaggggccct ttttcatcac cctggccatc
     3001 attttcgtca ccagctggtt cctcaagaag aactggctcg ataaactgac cgaagaatag
     3061 tctacttcca tcccggggcg gagcgcccgc cccgggatgc atctcgtccc tgtcaaggag
     3121 ttcctcatgc gccgccgctt tgcccttgtc gccctcgcat ttctgcttcc tgccgacctc
     3181 ctcgcccaag ccatcgcccc caccatcgcg cgccggagtg aaaacgcgaa ccggatcggc
     3241 ctatatgaaa aatacgagct tcagctcgaa ctgggcaatg tggtctatgc caatccctac
     3301 aaccccgaag agatcgacgt gcgcgccctc ttcacctctc ccggcggcag gcagtggacc
     3361 atttacggct tttatgacca ctatcagaac cgtaatgagt ggaaggtgcg attcgccccg
     3421 aatgaggcgg gcacctggag ctataccctc atggtgacca ctccggcggg gaacggcagc
     3481 agcccggcct acaccttcga ggcggccgcc tctgcacacc acggctggct gcacaccgcg
     3541 aaggcgaatc cacattacct ggtccatgac gacggcaccc ccttttacgg cgtcgctatc
     3601 ttctggccat gggggattgc ggaaccgggc ctgaacaccc tgcagagcct gggctgcaac
     3661 tgggtgggat tgtggaacat cacctacgac accggcaata ccctgatcga gtcgatgagc
     3721 tccggactcg gacgctatga ccagaacaag tgcaagcgga tcgacgacat cctcgaatgg
     3781 ctggaaaagc gtgatatggc cctttcgctc tcgatctggc cgcatgacct tttctgcaag
     3841 agcctctccg gctgggcgca gcgctggaac gacaatccct acaagcagct ttgcgatgtg
     3901 ctcgagattt atgagaacga gaccgcctgg cgctacatgg agaagcagta ccgctatatc
     3961 atcgcccgct ggggctacag ccgcgccatg ggtccctggg agatcatcaa tgagatcagc
     4021 ggcaccgacg cctgggccgc ggggcgaact gctgcagcgg acgagtggac gcgcaagtcc
     4081 cttacttttc tgcgggcgca cgatcctttc gaccggccgg tgacggcaag ccaacacggc
     4141 ggccttttct ggacggccgg ctatggcatc ttcgatctac ccaacatgca tctgtatgag
     4201 accgacggcg gcaccgcccg gtacgccagc aacccggtgc gcagcagtgt ctgggcctat
     4261 catgaggcag cccggcggat gtggcagggt tatgccaagc cggcgattct cggcgaggcc
     4321 ggggggggat cgaacaagaa tttcggcggc tactcccccg gctcggccga gtatacccgc
     4381 atgttccaca acgccctctg ggccagctgg tcgagcggac tggcggcctc gccgatgtgg
     4441 tggtctttca acacccatca gtcggacagc cgctttcagg agcagctcaa ggccttcagc
     4501 ctggtggctc cgggcatcga ttacgccctt cgccctttcc agcccgcgga ggtgaccgtc
     4561 gccggcgccg acgcctttgc catggaggac ggagagatgg ccttcggctg ggcgcgcgag
     4621 gagtggggcc gggatctctc catgcgctct ctgcagatcg ccgggctgcc ggattcggtg
     4681 tacagtgtgg tctattatga tacctggaag ggaacggcgg tggcgagcca catccggccg
     4741 gtggtggagg ggctgctcat cgacgagctg ccacagatcg ccgcttcggc cccggatctc
     4801 gccttcatca tccgcccggc cccgaccgga agcgtgccct ttcgcctggg gttgatggct
     4861 tcgccgcgcc ggctttacag cgacggcgag agctacgccg aggtgcgctg cctgatcttc
     4921 gatgtggagg gacgcttttg cagtgaggcc ggcagcatcc ttactttcaa cctcaacggt
     4981 cttgggcgtc tggtgggccc ggccgtcgtc gcagcggaga aggggctggc catgatcacg
     5041 gtgcagtcgg attccgccgg cagcggcgag gcacagatca tggtcgcctc tcccggtctg
     5101 atcggcgata ccctctccat cagcatgact gatcttcagc tgatcgatga tttcgagtct
     5161 tatgatccgg ccaatccggt cggcaatttc tggaagacga ccaccggcac ctttgccgcc
     5221 ctcatggtgg agccacggcc gggcatggcg gggggacagc ggttgcgcgt cgactatggc
     5281 atcggcggcg gcaaaccgcc ctatacaggt tttatttata ccctgccagc agccttgaag
     5341 ggcgccaaat tcctgcgctt ctatcttcag ggagatggct cagggcgcgg actggccgtg
     5401 cgcatcaacc gcaccagcag cagctactgg cagtacgaga tggccctgac atcgacggaa
     5461 gagagcatgg tcgagctgcc gctgaggggt tttttgccca gcgacggttc cagcccgctc
     5521 gatctcactc gggcactgag cctctccttc cacatcctca gggggagtgg cggcgacggc
     5581 tccgggacga tagccctcga tgatatcctc ttcgccaaca gctccacttc gggaatcgca
     5641 ggtgagaggg gggcgatttt gcccgaggcc ctgcagctgc tgcagaactg gcccaacccc
     5701 ttcaatgctt cgaccgaatt cagttttgtg ctgccgcggc ggatgcatcc gcagctgtcg
     5761 gtttatgata tacagggcag gagggtggca accctggcgg tgggggagcg cccggccggc
     5821 cgccaccgtc tggtctggca ggcg
//
LOCUS       DAOPWU010000214         5843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13620_length_5843_cov_3.898411, whole genome shotgun sequence.
ACCESSION   DAOPWU010000214 DAOPWU010000000
VERSION     DAOPWU010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13620_length_5843_cov_3.898411"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="PKM23_10595"
     CDS             complement(<1..1648)
                     /locus_tag="PKM23_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91961.1"
                     /translation="MNRYSTIFLVLLLVLLTGSLWAQNQAAVHWSLMLPDSGKVSAVT
                     GNARGLTQTHGDSFMCRDYANGPGPDQRWYPYNFNTKKAIMWGPDTTQNENIWVQFAA
                     HPKAYNDFHADSMTIYLGAKGTDKISVNLWYDTHADFSNPVALNATPLAMKKDGDSLF
                     TFILDKAVPEADTLYVRVYAWNAGPASNSKYLYMRLCTIYGTTTANPIPASATWALTS
                     ATGQTVATAGNVTAVDETLVNLNINQYTGYNGAQRLRIISAPGKNIWPANQTDQIDSV
                     YVQFAVSPKAGSNFTVTMLSMDLGCHSIDNFKANVWYSKDPDFLNPVQVVYQTADTLH
                     NWLPRNTSNTCQTVTAAPNLLILQGETFFLRIYPWVDNNPSESSGKYLTVQNVLIGGV
                     SESAPMPSSATWALTSATSQTVATSGKVNAADQWLNKMNINQYTGYNASQRTRIYTNS
                     WPANQTTQLDTVFAQFAVWPKSGAKLTVNSVSLNIGAHSTKTMKANIWYSTDSTFANP
                     VQIPYATADTVNNYLPDKDFPMAAVSAAPNVVVESGQRFYL"
     gene            complement(1680..2513)
                     /locus_tag="PKM23_10600"
     CDS             complement(1680..2513)
                     /locus_tag="PKM23_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HNY91962.1"
                     /translation="MKPRARTSAVALAALLLMAACSLMPCRREQTPVLRLIGDSTMAD
                     KPVEETPERGWGQLLPLFFRPEVKVVNYARNGRSTKSFITQGLWAQVLADLNEGDYLF
                     IQFGHNDAKVTDTTRYAEAHTAYKANLIRFIAEARARGARPLLLTSLARRYFSDEGVL
                     QDAHGDYPGVVREVAAQYGVPLIDHHARSMKLLAELGPERSERLFMRVPPGLFKGLPE
                     GKSDNTHFMEEGAAEMVKLAIAGLQELKHPLIKYLKPGAEWPQPQPPPWPIPLETPPK
                     P"
     gene            complement(2510..4009)
                     /locus_tag="PKM23_10605"
     CDS             complement(2510..4009)
                     /locus_tag="PKM23_10605"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HNY91963.1"
                     /translation="MLHADYSDPDVIRVGEDFYLVSSSFNHVPGLPVLHSRDLLHWEL
                     IGHALPRLPAPLYDRVQHGRGVWAPSIRHHTGEFRIYYCDPDLGLFLVKSKDPAGPWS
                     PPLLVREAKGWIDPCPFWDDDGRAWLVHAWAKSRAGFNSILTLHEMSPNGTRILDEGV
                     TVFDGHAHHPTIEGPKLYKREGWYYIFAPAGGVKPGWQTVLRSRSIQGPYEDRIVLAQ
                     GGTPVNGPHQGGWIETPAGDSWFIHFQDRGAYGRILHLQPMRWQEGWPVAGNDPDGDG
                     TGEPVLVHPAPALPPFAAVIPASDEFSGPALGLQWQWQANSDSAWYSLSEAPGQLRLY
                     AVQAPDTTGSLWLQPNLLAQKFPADSFTATAVMKGFSLQPGARAGLVIFGTDYACLTL
                     GAHQEGSLLRLSICRAADSGGEERVEEEAVLPSTSVSLRVQVSSGGRCAFSYSMDDHR
                     FIPIGGLFTAAAGKWVGAKVGLFAAGTAGGQHACGYADFDRIHFTGGEQ"
     gene            complement(4081..5079)
                     /locus_tag="PKM23_10610"
     CDS             complement(4081..5079)
                     /locus_tag="PKM23_10610"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY91964.1"
                     /translation="MTKFILTAFFAAAALTQAQVGLDKIAQSTMNFQQVGLSARASGV
                     GEAFCALGRGAEAIFYNPAGVVEAGRRVDLKFYSTQWIADINYMAGALRYDLGRYGAL
                     GLSFLNVDYGDIYTTSLLDASESALYPEGYKDTGLADNVGAWSLGLTYGRAISTQFLI
                     AGNARLVSQSLGTSNLAGGLTQNDATKLVFDAGVKYYTGIKSFRFGMAIRNFSSNLKR
                     EEINEQLPLTFTMGAALDLMDFTGGDKGPHSLTLSVDFVHPNNYSERVNAGLEYLFME
                     RVALRAGCQGNNDIASWSAGFGLHTPVGGNDIYIDYSYSFIDTFDSVSRFALGIGF"
     gene            complement(5119..>5843)
                     /locus_tag="PKM23_10615"
     CDS             complement(5119..>5843)
                     /locus_tag="PKM23_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91965.1"
                     /translation="RAKWNFDHGFRIPAAPPPVESVTITGLGTGVEIRWSAPEAESRP
                     DFAGYRIMRRLSNLDTLFFEEIHASGPEDKAAEHLFVDKEVLIGAQYYYYVQSRARIA
                     ADDPLADPLSRGRIIYSSRVMQPTINWINPPHFSQNDLTRIRIVPNPYNINDPLLVKQ
                     GWTDQRGIQFYNLPGKVTIKIFTEFGDLVQTIEHDSPVSAGYEIWDMITRNQQVISSG
                     VYIVVFQKPTGETAFQKFMVVR"
BASE COUNT         1124 a         1596 c         1943 g         1180 t
ORIGIN      
        1 tgagatagaa tctctgtccg gactcgacca ctacattggg ggcggctgaa acggcggcca
       61 tcgggaaatc cttgtccggg aggtagttgt ttaccgtatc cgccgttgca taaggaatct
      121 gtacgggatt ggcgaaagtg gaatctgttg aataccagat gttggctttc atggtcttgg
      181 ttgagtgcgc accgatgttg agcgaaacag aatttacggt taatttggcg ccgctcttcg
      241 gccagaccgc gaattgcgca aacaccgtat ccagctgagt ggtctggttg gcaggccagg
      301 agttggtgta gatacgggtg cgctgtgaag cgttatagcc ggtatactgg ttgatgttca
      361 ttttgttgag ccattgatcc gctgcgttca ctttccctga agtggcgacg gtctgactgg
      421 tcgcactggt cagcgcccag gtcgctgaag agggcatcgg ggcgctttcg ctcacgccgc
      481 cgatcaggac attctggacg gtgagatatt ttcccgaaga ttctgaggga ttattgtcga
      541 cccagggata gatacgcaga aaaaaggtct cgccctgcaa gatgaggaga ttgggcgctg
      601 ccgtcaccgt ttgacaggtg ttggaggtgt tacgcggcag ccagttgtgc agggtatcgg
      661 ctgtctggta gacgacctgg acgggattga gaaaatcggg atccttcgag taccagacgt
      721 tagccttgaa attgtcaatc gaatgacagc cgagatccat ggaaagcatg gtcaccgtaa
      781 agtttgaacc ggctttgggg gagacggcga actgtacata gaccgaatcg atctggtccg
      841 tctgattggc cggccatata ttcttgcccg gcgcggagat gatgcgcagc cgctgggcgc
      901 cattgtagcc ggtatactga ttgatattga gattgaccag ggtttcgtct acggcagtga
      961 cgtttccggc cgtagcgacg gtctgaccgg tcgcactggt cagcgcccag gtcgctgatg
     1021 ccgggatcgg gttggctgtg gtcgtcccgt aaatggtgca gagacgcata tagaggtatt
     1081 tgctgttcga ggccgggccg gcgttccagg cgtagacgcg aacgtagagg gtatcggcct
     1141 ccggaaccgc cttgtcgagg ataaaggtga agagcgaatc tccatctttt ttcatcgcca
     1201 ggggggtggc attcagagcg acgggattgg aaaaatcggc atgggtatcg taccagaggt
     1261 tgacgctgat cttgtccgtg cccttggcgc ccaggtagat ggtcatcgaa tcggcgtgga
     1321 aatcgttgta ggccttggga tgcgctgcaa actgcaccca gatattctca ttctgggtcg
     1381 tgtcgggccc ccacatgatg gcctttttgg tattaaagtt ataaggatac cagcgctgat
     1441 cgggtcctgg accgttggcg taatcacggc acataaagga atcgccgtgg gtctgggtga
     1501 ggccgcgggc gttgccggta accgctgaaa ccttgccgct gtccgggagc atcaggctcc
     1561 agtgcacggc ggcctggttt tgcgcccaaa ggctgccggt gagaagtacc agcagcagga
     1621 cgaggaagat ggtagagtaa cgattcatgc tacctccttg ttttgggaca gagtgtgaat
     1681 tatggtttgg gtggtgtttc cagcggaatt ggccagggcg gcggctgtgg ctgcggccat
     1741 tccgccccag gcttgagata tttgatcagg gggtgcttca actcttgcag tccggcgatg
     1801 gccagcttga ccatctcggc cgccccctcc tccataaaat gggtgttgtc cgatttgccc
     1861 tccggcaagc ccttgaaaag gccgggcggc acgcgcatga agagtcgctc cgatcgttca
     1921 ggtcccagtt ccgcgagcag tttcatgctg cgggcgtgat ggtcgatcag gggcaccccg
     1981 tactgagccg cgacctcccg gaccacgccg ggatagtcgc cgtgagcgtc ctggagaacg
     2041 ccctcatctg aaaagtagcg ccgggcgagt gaggtgagca ggagcggccg ggcgcctctg
     2101 gcgcgcgcct cggcgatgaa ccggatcaga ttggctttgt aggcggtgtg cgcctccgcg
     2161 taacgggtgg tatctgtcac cttggcgtca ttatggccga actggatgaa gaggtaatcc
     2221 ccttcattca gatcggcgag aacctgagcc cacaatccct gggtgatgaa gcttttggtg
     2281 ctgcggccgt ttctggcgta attgaccacc ttgacctccg gccggaagaa caggggcaat
     2341 agctggcccc agccccgttc gggggtctcc tcgaccggct tgtcagccat ggtcgaatcg
     2401 ccgatcaggc gcagcacggg cgtctgctcc cggcggcagg gcatcaggct gcaggcggcc
     2461 atgagcagga gcgcagccag ggcgacggct gaagtccggg cgcggggctt cattgttccc
     2521 ctcctgtaaa atggatgcgg tcgaaatcgg cataaccgca ggcgtgttgg ccgccggctg
     2581 tcccggcggc gaaaagaccc actttggcgc cgacccattt gcccgcagcg gcggtgaaga
     2641 gtccgccgat cggaataaag cgatggtcat ccatgctgta gctaaaagca caccggccgc
     2701 cggacgaaac ctgcacccgc aagctcactg atgtggaggg cagcactgcc tcctcctcca
     2761 ccctctcctc tccacccgag tcggccgccc ggcagatgct cagccggaga agagagccct
     2821 cctgatgagc cccgagggtc aggcaggcat agtccgtgcc aaagatgacc agacctgccc
     2881 tcgccccggg ttggagtgaa aaacccttca tgacagccgt cgcggtgaat gaatcggctg
     2941 gaaatttctg cgccagcaga ttgggttgca gccacaggct gccggtggtg tccggcgcct
     3001 gtacggcata gagcctgagc tggcccgggg cctccgagag gctgtaccag gcgctgtcgc
     3061 tgttggcctg ccactgccat tgcagcccca atgccgggcc ggagaactcg tcggaggcgg
     3121 ggataacagc ggcaaacggc ggcaaggccg gcgcagggtg aaccagcacc ggctcgccgg
     3181 tgccgtcgcc gtcgggatca ttccctgcca ccggccatcc ctcctgccag cgcatcggct
     3241 ggagatggag gatgcgtcca taggccccgc gatcctgaaa gtggatgaac caggaatcgc
     3301 cggcgggggt ttcaatccag ccgccctggt gcggcccgtt gaccggggtg cccccctggg
     3361 cgaggacgat cctgtcttca taggggccct ggatcgagcg ggagcgcaga acggtctgcc
     3421 agccgggttt taccccgccg gccggggcga agatgtagta ccagccctct cttttgtaca
     3481 attttggccc ctcgatggtc ggatgatggg catggccgtc gaatacggtc accccctcat
     3541 cgaggatgcg ggtgccattc ggactcattt cgtgcagggt cagaatcgag ttaaagcctg
     3601 cgcggctttt ggcccaggca tggaccaacc aggcccggcc gtcgtcgtcc cagaacgggc
     3661 agggatcgat ccagcctttc gcttcccgca cgagcaaggg cggcgaccag gggccggcgg
     3721 gatcctttga cttgaccagg aagagcccta gatcgggatc gcaataatag atccggaact
     3781 ctccggtgtg atgacggatg ctcggcgccc atacccccct gccgtgctgt acccggtcgt
     3841 agagaggcgc gggcaggcgc ggcagggcat ggccgatcag ctcccagtgg agcaggtcac
     3901 gtgagtgcag caccggcagc cccggcacat ggttgaagct ggaggagacc aggtagaaat
     3961 cctcccccac gcggatcaca tccggatcgg agtaatcggc gtggaggatg ggattggtat
     4021 atgtcccctg cgccagggcg cccgcagtga gcaggacgag cagcagggtg agcgcctttc
     4081 tcagaatcct atccccagtg cgaaacggct gacactgtca aatgtatcga taaatgaata
     4141 tgaataatcg atatagatat cattgccccc gacgggggta tggaggccga atccggccga
     4201 ccatgaggcg atgtcgttat tgccttgaca gccggcgcgc agcgccaccc gctccatgaa
     4261 gaggtactcc agtccggcgt tgacccgttc ggaatagtta ttcggatgga cgaaatcgac
     4321 ggagagggtg aggctgtggg gtcctttatc cccgccggtg aaatccatga ggtcgagggc
     4381 cgcccccatc gtgaaggtga gaggcagctg ctcgttgatc tcctcgcgct tgagattgga
     4441 ggaaaaattg cggatggcca tgccgaaacg gaagctcttg atcccggtat agtatttgac
     4501 gccggcgtcg aagaccagtt tggtggcgtc gttttgggtc agaccgccgg cgaggttgga
     4561 ggtgcccagg gactggctga ccaggcgggc gttgccggcg ataaggaact gggtgctgat
     4621 ggcgcggcca taggtcaggc ccagcgacca ggcgccgacg ttgtccgcca gtccggtatc
     4681 cttgtagccc tcggggtaaa gggcgctctc ggaggcatca agcagagagg tggtatagat
     4741 atcgccgtaa tcgacattga gaaaggagag gccgagcgcg ccatagcggc cgaggtcata
     4801 tctgagggcg ccggccatat agttgatgtc ggcgatccac tgcgtggagt agaatttaag
     4861 atccacgcgc ctgccggctt cgaccacccc ggccggattg taaaagatcg cctccgcgcc
     4921 gcggccgagg gcgcaaaagg cctcgcccac tccgctcgcc cgcgccgaga ggccgacctg
     4981 ctggaaattc atcgtgctct gggcgatctt gtcaagcccg acctgggcct gtgtgagggc
     5041 cgcagcagcg aagaaagcgg tcagaataaa ctttgtcatg gtgtatggtt tcatgagagc
     5101 attctccttg gctccggatc accggacgac catgaatttc tgaaaggcgg tctcgccagt
     5161 gggcttttgg aagacgacga tgtaaactcc gctgctgatc acctgctggt tgcgggtgat
     5221 catatcccag atctcgtagc ccgcgctcac cgggctgtca tgctcgatgg tctggacgag
     5281 gtcgccgaac tcggtgaaga tcttgatcgt cactttgccg gggagattgt agaactggat
     5341 gccgcgctga tcggtccagc cctgcttgac caggaggggg tcattgatgt tgtagggatt
     5401 gggcacgatg cggatcctgg tcaggtcgtt ttgggaaaag tggggcggat tgatccagtt
     5461 gatcgtcggc tgcatgaccc ggctgctgta gatgatgcgg ccgcggctga ggggatcggc
     5521 gaggggatcg tcggcggcga tgcgggcgcg ggactgcaca tagtagtagt actgggcgcc
     5581 gatgaggacc tccttgtcga cgaaaagatg ctcggccgcc ttgtcctcgg gcccgctcgc
     5641 atggatctcc tcgaaaaaga gggtgtcgag attggagagc cggcgcatga tgcggtagcc
     5701 ggcgaaatcg gggcggctct cggcttcggg ggcgctccag cggatctcga ctccggtgcc
     5761 gaggccggtg atggtgaccg attcgaccgg gggcggggct gcagggatgc ggaagccgtg
     5821 gtcgaaattc cacttggcgc gcc
//
LOCUS       DAOPWU010000215         5790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13790_length_5790_cov_5.698344, whole genome shotgun sequence.
ACCESSION   DAOPWU010000215 DAOPWU010000000
VERSION     DAOPWU010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13790_length_5790_cov_5.698344"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..1576)
                     /locus_tag="PKM23_10620"
     CDS             complement(374..1576)
                     /locus_tag="PKM23_10620"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNY91966.1"
                     /translation="MESIREIYKIGHGPSSSHTMGPRKAAERFREKNSQADHFKITLF
                     GSLAATGKGHLTDLVIHNVFSGRRVEIKWEPGIFLPRHPNAMEFAAYDQAGSLTDRWV
                     AYSVGGGAVVDDASGPETVPIYPHTTMDSILKYCRDNGLKFWEYVEQVEGPEIWDFLK
                     HIWDAMQESIHRGLLSDGILPGSLKLPRKARQSRAKADQYSGPFKRMAQIFSYALAVS
                     EENAAGGVIVTAPTCGASGVLPAVLYYHKKVYKTEKEVILKALSTAGLIGSLVKANAS
                     ISGAEVGCQGEVGTACAMASAAAAQIMGGTINQIEYAAEMGLEHHLGLTCDPVEGLVQ
                     IPCIERNAFGAQRALAHNTYALMSDGRHRISFDEVVKTMYETGISLQSQYRETSLGGL
                     ALRDARPT"
     gene            1641..1904
                     /locus_tag="PKM23_10625"
     CDS             1641..1904
                     /locus_tag="PKM23_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91967.1"
                     /translation="MLRVKKIDAIIPGQAPDTRRFSHFNSTRPSPAPTWLITSGKISQ
                     HKPEKQTKFPEHATSNRPVYMLLDNFRLLFRVVLANIPAGWKR"
     gene            2411..2773
                     /locus_tag="PKM23_10630"
     CDS             2411..2773
                     /locus_tag="PKM23_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91968.1"
                     /translation="MAVVGIAIVVGFTMFHNAADAAQIDTVSQECTGLAVRAQAYYLT
                     PAYRGGGGNTFSGLTLTRVAPASANFPAGQTEDGIYEIQVQPQTVTITGTPERPRAAA
                     RVVTAVVTSSLITLTVAD"
     gene            complement(2817..5132)
                     /gene="cysS"
                     /locus_tag="PKM23_10635"
     CDS             complement(2817..5132)
                     /gene="cysS"
                     /locus_tag="PKM23_10635"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNY91969.1"
                     /translation="MNIHGNILDLIGNTPLLKINRLAPRPPVEIYVKLESCNPGGSIK
                     DRVALAMIEAAERSGELTHDKTIIEATSGNTGIGLAIVARVKGYRLTLAMSEAASEER
                     RKILKAYGAEILLTPARMGTDGAIEEVYRLVRENPAKYYNPDQFNNPASIEAHYQGTG
                     REIWEQTEGRVTHVVAALGTTGTIMGITKRLKELNPAIRTIGMEPYLGHKIQGLKNMK
                     ESYRPGIFDKHLLDRIVHIEDEEAFATARALAREESLFVGMSSGAAMAAARKIAAELN
                     EGLIVVILPDGGERYLSTKLFVERTESAFRFYNTLTRRKESFEPAEPGVVKMYTCGPT
                     VDGPPHLGNCRRFVVADLVRRYLEHKGLEVRHVVNITDLDDRTIRGAELAGQPLAEFT
                     RLHTEKFLQDMKCLGVTPATYYPKASEHVMEMSELTEKLISKGFAYEKLRSVYFDISR
                     FRNYGKLSGMDLGQIQPGMKTDLENYEKDNPRDFTLLKRTTLAELKKGIFFQTRWGNV
                     RPGWHIECAAMSMKYLGESFDIHTSDTDLIFPHHENEIAISEAATGKPFARYWLHNAL
                     VMVEGKKISGLADMPLVLQDLLDLGYSGREVRYWLLAAHYRKSLNYSVAALETARIAL
                     RKIDNFTRSLAKTHPGSHAPEAEQSVYDLRHRFEAAMDDDLNTPAALATLFDFIRSMN
                     RLIDKGQLDQSGIDRTLQALDEINRVLNIMVIGAEEKEDEEIQQLIEERETARRAQDW
                     STADRIRSQLFEKGVIVRDSPAGPRTHHTAS"
BASE COUNT         1276 a         1553 c         1578 g         1383 t
ORIGIN      
        1 atgctcatgc gagaaaaaaa gtggaatctg aaagccgtac tgcgtacact acttaatata
       61 tgatttcccg ggcaaaaatc aaaatgccat gctcaacatc tgaccggatc gcatcaatga
      121 caggaaaagg agtgccatga cgactatttg tcccgccact tgcgggaccg ataacattat
      181 cgtgccatgg gaacgctccg ccagccacaa accacccgga taaaaaaggc ccctgctcgg
      241 ttgacctgat caggggcctg gttgtttcca ggtggattaa tggcgaagga ctgggttacc
      301 tgcgattggg gttgtaccgc accggtttgc cggccacccg ttcagtcgga ttgttgatat
      361 gcatcgaaat cttttatgtc ggccttgcat ccctcaatgc cagtccgccg agggaagttt
      421 cgcggtattg gctttgaagg ctgatcccgg tttcatacat ggttttcacc acttcatcaa
      481 agctgatccg gtgacggcca tccgacatga gggcataggt gttgtgggcc agggcgcgct
      541 gtgctccgaa ggcatttctt tcaatgcagg gtatttgcac cagtccttcc accggatcac
      601 aggtcaatcc aaggtgatgt tccaatccca tttcagcggc gtattctatc tggttgatgg
      661 tgccgcccat gatttgcgcc gcggcagcgg aagccatcgc acaggccgta cccacttccc
      721 cctggcagcc aacctcagcc ccggagatgg aggcattggc ttttaccaaa ctgccgatca
      781 atccggcagt ggacaaggct ttgagaataa cctccttctc cgtcttgtac acctttttat
      841 ggtaatacag caccgcaggc aaaactccgc tcgcaccgca ggtgggagcc gtgacaatta
      901 cccccccagc tgcattttct tcggagacag ccagggcata ggagaaaatc tgcgccatac
      961 gcttaaaggg accggaatac tgatctgctt ttgccctgct ttgcctggcc ttgcggggga
     1021 gcttcaagct gccgggtaaa ataccgtccg agagcagtcc gcggtgaatg ctctcctgca
     1081 tcgcatccca gatgtgtttt aaaaaatccc aaatctccgg accttcgact tgctcaacat
     1141 attcccagaa tttcagtccg ttatcacgac agtatttcag aatggaatcc atggtggtat
     1201 gcggatagat cgggacggtt tccggcccag aggcatcatc caccacggcg cctcccccca
     1261 cactataagc cacccatcga tccgtcaatg atccggcctg gtcatatgcc gcaaattcca
     1321 tggcgttggg gtgcctgggc aaaaaaattc cgggctccca tttgatttcc accctccggc
     1381 ccgaaaaaac gttatgaatg accagatccg tcaggtgtcc cttgccagtg gcggccaggc
     1441 tgccaaataa ggtaatttta aaatggtcag cctgactgtt tttttcgcga aaccgttcag
     1501 cggccttccg tggtcccatg gtatgactgc ttgaaggccc gtgtccgatt ttgtaaatct
     1561 ctctgatcga ttccataatg ctggcagaat aattgatttt gcaaaggggg gaaaagcgaa
     1621 caacaccagg agatcaggtt atgctgaggg tcaagaaaat cgatgccatc atccctggtc
     1681 aggcgcctga tacccggcga ttttcccatt tcaacagcac ccggccatct ccggcgccca
     1741 catggttaat aaccagcgga aaaattagcc aacataaacc tgaaaagcaa acaaaattcc
     1801 cagagcatgc aaccagtaat cggccggttt acatgctttt agataatttt cgcttgcttt
     1861 tcagagttgt gttggctaat attccggcag gctggaagcg atgaggtcga cggatatggc
     1921 ccggcgcttt gagaatgggg aaatggtggc gtatcgggcg tttgacaggg gatgatttcc
     1981 tcgatttttt gggaccgccg gcaaaatcag aactcctgtc agttggccga cacccggtgc
     2041 acaccacgaa ctatattctt gagatcgagg agagcaagtg caatggatgc ggccagtatg
     2101 tggatgcatt tccggctgag gcgatggcgc attacctggc gagctgggta atgcccctcc
     2161 ggtcaagcag gttcgggccg gccggcagat gaggtcgaga tttgttgaat ggctggtcca
     2221 caggcgggtg cgctaaacgc ttgacaaagg caggatttaa actagctgct cactctgccc
     2281 agacaacatt ccttccggat cacgccgttt gcgagcgact ggcagccgga tccggtgtac
     2341 tcatgaacca ggggaatttt gaggtaagca ggagatgggg caacaacaat tattacttct
     2401 cgtcatcgcc ttggctgtcg tcggcatcgc catcgtggtc ggcttcacca tgttccataa
     2461 cgccgcggat gcggcccaga ttgatactgt aagtcaggag tgtacagggc tggcggtcag
     2521 ggcacaggcc tactacctca ccccggccta tcgcgggggc ggcggcaaca ccttctcggg
     2581 attaaccctg actcgagtag cgccagcctc ggccaatttc cctgccggtc aaaccgagga
     2641 cggcatttac gagatacaag tccagccgca aaccgtcacc atcaccggca cgccggaacg
     2701 tccccgcgct gctgcacgcg tagtgactgc ggtcgtgacg tcttcgctca tcaccctgac
     2761 cgtcgctgat taagcctctt tgcccccacc gtgctggaca aaaccttggt ttcttcttaa
     2821 ctcgcagtat ggtgggtgcg tggtccggcg ggcgaatcgc gtacaatgac tcctttttcg
     2881 aaaagttgac tccgaatacg gtccgcagtg ctccagtctt gcgctcgccg ggccgtttcc
     2941 cgctcttcga tgagctgctg gatctcctcg tcctccttct cttccgctcc aatgaccatg
     3001 atattgagaa cccggttaat ctcgtccaat gcctgtaaag tccggtcaat cccggactgg
     3061 tctaactggc ccttgtctat cagccggttc attgatcgta tgaaatcaaa gagggtggcc
     3121 agcgcggctg gagtgttgag atcgtcatcc atagccgctt caaagcggtg ccggagatcg
     3181 tagacggatt gttccgcctc cggcgcatgg cttccggggt gggttttagc cagcgagcgg
     3241 gtaaaattgt cgattttgcg cagggcgatt cgggcggtct ccagcgcggc aacggaatag
     3301 ttcagggatt tgcgataatg tgcggcgagc agccagtacc gcacttcccg gccggaatag
     3361 ccaagatcga gcaggtcttg caggaccagc ggcatgtcag ccaggcccga tattttttta
     3421 ccttccacca tcaccagggc gttgtggagc cagtagcggg cgaacggttt cccggttgcg
     3481 gcctcgctta tggcgatttc gttctcatgg tgcggaaata tcaggtcagt gtcgctggtg
     3541 tgaatatcga aggattcccc caggtacttc attgacatcg ccgcacattc gatatgccag
     3601 ccaggacgga cgttgcccca gcgcgtctgg aaaaagatcc ccttcttgag ttccgccagg
     3661 gtggtccgct tcaaaagggt aaagtcgcgc ggattgtcct tttcataatt ctccagatcc
     3721 gtcttcatgc cgggctggat ctgtccgaga tccatgccgg agagcttgcc atagttcctg
     3781 aagcgggaga tgtcaaaata gacggagcgg agtttttcgt aggcgaatcc cttgctgata
     3841 agcttctcgg tgagttccga catctccatg acatgctcgc tcgccttggg ataatacgtg
     3901 gccggagtta caccgaggca tttcatgtct tgcagaaatt tttccgtatg caagcgggtg
     3961 aattctgcca gaggctgacc cgccagctcg gcaccccgga tggtccgatc atcgagatcc
     4021 gtaatgttga caacatggcg cacctcgagc cctttgtgtt cgaggtagcg gcgcaccaga
     4081 tcggccacga cgaagcggcg gcagttgccc agatggggtg gaccatcaac ggtgggcccg
     4141 caggtgtaca ttttgacgac accgggctcg gctggctcga acgactcctt tcggcgggtg
     4201 agggtgttgt agaagcggaa ggcggattca gtccgctcca cgaagagttt ggtggagaga
     4261 tagcgctccc cgccgtcggg caggattacc acgatcagcc cctcattgag ctcggcggcg
     4321 atcttccggg ctgctgccat cgccgcgccg ctgctcatcc cgacgaaaag gctctcttca
     4381 cgggccagcg ccctggctgt cgcaaaggct tcttcatcct cgatgtggac gatccggtca
     4441 aggaggtgct tgtcgaagat accggggcgg taggactctt tcatgttctt gaggccctgg
     4501 atcttatgcc ccaggtaggg ctccatgccg atggtccgga ttgccggatt gagctccttg
     4561 agccgtttgg tgatccccat gatcgtccct gtcgtgccca gggcggcgac gacatgagtg
     4621 accctgcctt cggtctgttc ccagatctcc ctcccggtgc cctggtagtg ggcctctatg
     4681 ctggcgggat tgttgaactg gtccgggttg tagtattttg cagggttctc gcggacaagg
     4741 cgatagactt cctcaatcgc tccgtcggtg cccatccggg cgggagtcag gaggatttcc
     4801 gctccgtagg ccttgaggat cttgcgccgt tcctcactcg ccgcttcgct catcgccaga
     4861 gtgagccgat agcccttgac tcgcgcgacg atagccagac cgatccccgt gttgccgctc
     4921 gtggcctcga taattgtctt gtcatgtgtg agttcacccg aacgttccgc agcttcgatc
     4981 atcgccaggg cgacccggtc cttgatcgag ccgccgggat tgcaggattc cagtttgaca
     5041 taaatctcga ccggcggtcg aggtgcaagc cggttgatct tgagcagggg cgtgttgccg
     5101 atgaggtcga gaatgttacc gtgtatattc ataggatgaa ctctccgtat aatgtgaaaa
     5161 tatccaaccg gagtgtgaaa atcaagcaaa atcatacaaa tgagtcaagc cagcagcgcc
     5221 gtgataccgt ggcgtgcaag gggtcccgga tcaactacaa gcgaatggat gaaaattgca
     5281 gcgcctccat tcgctgcgtc cggtaggcac aagtgtaaca aataagcgaa agtggatcag
     5341 gcttttaaat gaaaatcctg gactaaaact ggactaaagg atgtcccaat atgggtaaaa
     5401 tggaggtatt ataaaaagaa aagagctctg aattttcaga gctctttctt taaaaagcct
     5461 gtatttactt cacttatctt tgcggggctg acggggctcg aacccgcgac ctctggcttg
     5521 acaggccaag aaaatacgga taaaaaatgg gctaactaat tgatttttag atcggacttt
     5581 caaatcctgg gctaaaactg ggctaaaaaa tcggtacttt caatatattg aatattctgg
     5641 tatggaaata ttgggggctt tcaatcggca tcatttgagt gtatccgaat tttatcaact
     5701 tcaattatcc atattgaacc tgctggatcg gctgattttc aggttataat aaaattgttc
     5761 agtaccgtgt ccgctctatt gggataaatt
//
LOCUS       DAOPWU010000216         5771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13852_length_5771_cov_4.503674, whole genome shotgun sequence.
ACCESSION   DAOPWU010000216 DAOPWU010000000
VERSION     DAOPWU010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13852_length_5771_cov_4.503674"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKM23_10640"
     CDS             <1..901
                     /locus_tag="PKM23_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNY91970.1"
                     /translation="TARAIVDGYVASTFQEGDEQFDVRVRLNKEDRTSLENVGDLLVK
                     SSKDIGNGQRLMVPIRNIAAIQPASGPSKINRYDRQREIRVDANTYQKTLGQVMGGIQ
                     QQTRKMAIPAGYAIGVVGQGQMQSETFANMLMALALAIIFVYIVLAAQFESFIHPFSI
                     MLALPMSLIGAVLALLAWGSSLSMMSMIGIIMLMGLVTKNGILLVDYANQLRRQGVAR
                     TQALIQAGAIRLRPILMTTFAMIFGMIPVAFALGEGSEFRAPMGQAVIGGLITSTLLT
                     LFIVPVVYSILDDFSSRKQREEK"
     gene            complement(927..1373)
                     /locus_tag="PKM23_10645"
     CDS             complement(927..1373)
                     /locus_tag="PKM23_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91971.1"
                     /translation="MKGIALFGGIALLVLAAMAGLVFLVIILARMMGGIFRRASGWEV
                     LARQFPGPEQAPAETRTGAVKVGSVYFRYGPRFGPTPQGLYLVYKSVYSYPSLLIPWN
                     ALYNPKKTLLFWRAARSYEIGRPAVTTLTLWEEVWKGLKPDLPAAG"
     gene            1520..2389
                     /locus_tag="PKM23_10650"
     CDS             1520..2389
                     /locus_tag="PKM23_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNY91972.1"
                     /translation="MTNRTFQGFSRDTVSFFHELEENNERAWFEANRERYQLEVVQPA
                     QAFVLAMNEPLHSVYPGLQADPRPNGAGSIFRIHRDTRFSRDKRPYKTNLGIFFWHGW
                     QGKRERPGYYVHLEGNSLALYCGLYEFSSEKLLNYRRAAAPDTKSGSAQACANPFFRI
                     LFKGCHALLQGFGKGFAQGAGQQRGHNSIEALPGPHAGAFVGPGQGPDMDRPDAHRSP
                     GPDDAHRRGNFFDSRTNSHTAGLPAEFRPDCDLSLQMHLLDALIPFWPFFQVGQYLPD
                     DLNGCLDLCLTLC"
     gene            complement(2465..2608)
                     /locus_tag="PKM23_10655"
                     /pseudo
     CDS             complement(2465..2608)
                     /locus_tag="PKM23_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
     gene            3145..4710
                     /locus_tag="PKM23_10660"
     CDS             3145..4710
                     /locus_tag="PKM23_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNY91973.1"
                     /translation="MRFNNRLQQLFRSEVWWVWCIAGLKLILNVAFHGRYGYFRDELY
                     YIACSDHLAWGYVDQPPLCALILAATRWLFGDSLYAIRFVPALAGAGVVVLAGSMARL
                     LGGKKFAQLLAAAAAALSPVILGNAARYYSMNAFDLFFWALLAWIVTKIIVQERPDWW
                     IWFGVAAGLGLLNKYSVGFLVAGLGAGLLLTAQRRHLLDRRFWLGALIASLLFLPHIL
                     WQVNNDFPSLEFMRRAAGEKNVALAPLEFLIGQFMQSGFAQSLLWLLGLAFFGFHPSA
                     RKVRLFAWAFVLILAVMLLTHAKVYYLTPVYMPYIAAGAVLMERVRWQGVRPIFTIAL
                     VLLSALVMPFALPVLPVDKFVAYQNALGITPKAEEHSPLEELPQYYADMFGWEEMVGQ
                     VAAIYRQLTPEEQTGCVIYVRNYGQAAAIDFFGRRYGLPPALCPHNNYWLWGRSRLNQ
                     PMTVAIIMGAHRTTEENLADLQGPGRFTQVELAATTHHSYAMPHENGRQFFICRGPQF
                     SLAQIWPGEKEFI"
     gene            complement(4711..5040)
                     /locus_tag="PKM23_10665"
     CDS             complement(4711..5040)
                     /locus_tag="PKM23_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91974.1"
                     /translation="MIGSGGVSRNFQVFDALEFLVEVTAHIPDRGKRLIHYLGWYSDK
                     ERGVLKKMEPSSKAKADGDNAEADAFLKQLPSPQQQLRGQASRQRGLIDPMVYEGSLP
                     TVQGPGR"
     gene            complement(5464..>5771)
                     /locus_tag="PKM23_10670"
     CDS             complement(5464..>5771)
                     /locus_tag="PKM23_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY91975.1"
                     /translation="LPLTTTIEARELSSSRTMLFNAAGNPVRSRTTIQYQLDGAMPVE
                     IAVFNLLGQKVATLINEMNQPGLHRVTWHTAGHPPGLYFYRMRTPKEILTKKMVVIN"
BASE COUNT         1139 a         1730 c         1576 g         1326 t
ORIGIN      
        1 caccgcgcgc gccattgtcg atggctacgt cgccagcaca tttcaggagg gcgacgaaca
       61 attcgatgtg cgtgtccgtc tgaacaagga ggaccgcacc tcactggaga atgtcggcga
      121 cctcctggtc aagagcagca aggatatcgg caacggccag cggctgatgg tgccgatccg
      181 caatatcgcg gcgattcagc ccgcttcggg gccctccaag atcaaccgtt atgaccggca
      241 acgtgagatt cgcgtcgatg ccaacaccta ccagaagact ctggggcagg tcatgggcgg
      301 gatacagcag cagacccgca agatggcgat tcccgccggc tatgccatcg gggtggtggg
      361 acaggggcag atgcagtccg agacctttgc caacatgctg atggccctgg cgctggcgat
      421 catctttgtc tatatcgtgc tcgccgccca gttcgagagc tttatccatc ccttctcgat
      481 catgctggcg ctgccgatgt cgctgatcgg tgcagtgctg gcgctgctgg cgtggggcag
      541 ttcgctctcg atgatgtcga tgatcggtat catcatgctg atgggtttgg taaccaagaa
      601 tggcattttg ctggtcgatt atgccaacca gctgcgccgg caaggggtgg cgcggacgca
      661 agcgttgatc caggccgggg cgatccggct gcggccgatc ctgatgacca catttgccat
      721 gatcttcggc atgatcccgg tggccttcgc cctcggcgag ggcagcgagt ttcgtgcccc
      781 gatgggccag gcggtgatcg gcggcttgat cacctccacc ctgctgaccc tcttcattgt
      841 gccggtggtc tattcgattc tcgacgactt cagcagcaga aagcagcgcg aagaaaagta
      901 accgcaccac ctcatgcgca gacccctcac cctgctgcgg ggagatcggg cttgagccct
      961 ttccagacct cctcccagag cgtgagggtg gtaacggccg gcctgccgat ctcataggat
     1021 cgtgcggcgc gccagaagag cagcgtcttt ttcggattgt acagggcgtt ccaggggatg
     1081 agcagagaag gatagctgta gacgctttta tagaccagat agagcccttg cggggtggga
     1141 ccgaagcgcg gtccgtagcg gaaatagacg ctgccgacct tgacagcgcc ggtgcgggtt
     1201 tcggccggcg cctgttctgg cccggggaac tgccgggcca aaacttccca accgctggcg
     1261 cggcgaaaga tcccgcccat catccgggcc aggatgataa ctagaaaaac cagtcccgcc
     1321 atggcggcca ggacgagcag ggcaattcca ccaaaaagag ctataccttt catattctca
     1381 cctcatttta attcgggatt ttccgtatct ttaatcaata gagcaggccg tgccgatccg
     1441 aaaccatacg cctgcagatg ttcaacactc cagcatccac agtagcgttt tcaaactatc
     1501 tcaaacgagc gcgaaggcca tgacaaacag aacttttcag ggtttcagcc gcgatacggt
     1561 cagttttttc cacgagctgg aggaaaacaa tgaacgcgcc tggttcgaag ccaaccggga
     1621 gcgataccag ttggaggttg tacagccggc gcaagccttt gtcctggcca tgaacgaacc
     1681 tttacactcg gtttatcccg gtctgcaggc cgatccccgg cccaacggtg ctggctcgat
     1741 ctttcgcatt caccgggata cccgcttcag ccgcgacaag cgtccctaca aaaccaacct
     1801 cggcatcttt ttctggcacg gatggcaggg caaacgcgaa cggcccggct actatgtaca
     1861 tctggaaggc aacagtctgg ctctctactg cggactttac gaattttcca gtgaaaagct
     1921 gctgaactac cgccgcgcag cggcgccgga tacaaagtca ggatctgctc aagcctgcgc
     1981 gaaccctttt ttccgcatcc tttttaaggg ctgccatgcc ctgctgcagg gctttggcaa
     2041 gggatttgcg caaggggctg gccaacagcg aggccacaat tccatagaag cactccccgg
     2101 tccacacgct ggtgctttcg ttggtcccgg tcagggccca gacatggatc gcccggatgc
     2161 ccatcgttcg cccggaccag acgatgctca ccggcgaggt aatttcttcg attcgcgaac
     2221 gaattctcat accgccggtc ttccagcgga attccgtccc gattgcgacc tctccttgca
     2281 gatgcatctc ctggacgctc tcattccatt ctggccattt ttccaggtcg gtcaatactt
     2341 gccagacgat ttgaatgggt gccttgatct ctgcctcaca ctctgctaaa accggtgcta
     2401 cttcattatc ttttatctga tccaaatcga ttctcctctc cgtacgggta ttggatttgc
     2461 cgtgctagac cttgagcagg ccgcgaaagc cgcgggtggc ataatacgac tcagcgctgt
     2521 tgtgatagac aaagacatgg ttatagcggc gatcgcagaa gagggccccg cccagcgccc
     2581 tgatggccgc cggcgtcttg attaaggcat catgcactgc tgtttccatc ccggaaatct
     2641 ctttgttctg gttgcctgga ttatccatac atccccatcc tggcccacac ttcaatatgc
     2701 atacgtttta tccgtttcaa cagtccatgc tgtctattca ttccgcatgc tgactccagc
     2761 tccacctttc attttatgta aaaagcatac aagtactggg tggtattgat ctaatagaca
     2821 caagggtcag gtctgacttt aatgctggcc gaggaaaagg cgtcaatcct ggatagcagc
     2881 gtcgttgtca tccggcccaa tcctgtcaat caacctcgtt ttcaatttcc ctgtaactgt
     2941 aagatcgagc gttcgtacaa tttaagggcg tatattatcc tgctaatctc atcgcactgc
     3001 aaacgggcct caatcctttc acgcagaact cgcatacggt gacgctcttg accacggcag
     3061 tatgcggccg ggcggccatg gaagccgcgg gctgccaatc aggtcggacg tgatctaatc
     3121 cgttatccac atggagggag aaccatgaga ttcaataatc gtcttcaaca actcttccgc
     3181 agcgaagtct ggtgggtatg gtgcattgcg gggctcaagc tgatcttgaa tgtggccttt
     3241 cacggccgtt acggctactt tcgcgatgaa ctttattaca tcgcctgcag cgaccatctc
     3301 gcctggggct atgtggatca gccgcccctg tgcgccctga ttctggccgc gacacgctgg
     3361 ctgttcgggg attccctcta cgccatccgt tttgtgcccg ccctggccgg cgcgggtgtg
     3421 gtggtgctgg ccggttcgat ggcgcggctg ctgggcggga aaaaattcgc acagctgcta
     3481 gccgctgccg cggccgccct ctcaccggtc atcctcggca acgccgcgcg ctattattca
     3541 atgaacgcct tcgatctttt tttctgggcg ctgctcgcct ggatcgtgac caaaatcatc
     3601 gtgcaggagc gtcccgactg gtggatctgg tttggggtgg ccgcaggact ggggctgctc
     3661 aacaagtatt cggtgggctt tctggtcgcc ggccttggcg ccggactgct gctcaccgcg
     3721 cagcgtcggc atctgctcga ccgccgcttc tggctgggtg cgttgatcgc ctcgctgctc
     3781 ttcctgcccc atatcctctg gcaggtgaac aacgatttcc cctcgctgga gttcatgcgc
     3841 cgcgctgcgg gtgagaaaaa tgtcgccctg gcgcccctcg aatttctcat cgggcagttc
     3901 atgcagtcgg gattcgctca atccctgctc tggctgctcg gcctggcctt cttcggcttt
     3961 catccttcag ccagaaaagt gcggctgttc gcgtgggctt ttgtgctgat cctcgccgtg
     4021 atgctcctca cccacgccaa ggtctattat ctcactcctg tttacatgcc atacatcgcc
     4081 gccggcgccg tgctgatgga gcgggttcgc tggcaagggg tgcggccgat tttcacgatc
     4141 gcactggtcc tgctcagtgc gctggttatg ccctttgctc tcccggtcct gccggtggat
     4201 aaattcgtcg cctaccagaa tgccctggga ataacaccca aagcggagga gcattcaccg
     4261 ctggaggagc tgccccagta ttatgccgac atgttcggct gggaggagat ggtcgggcag
     4321 gtcgcggcga tttaccggca gctgacgccg gaggagcaaa ccgggtgtgt gatctatgtg
     4381 cgcaattatg gccaggcggc agctatcgat ttcttcggcc gccgttatgg actgccgcct
     4441 gcgctctgtc cgcacaacaa ctactggctg tgggggcgga gccgcctcaa tcagcccatg
     4501 actgtggcca tcatcatggg cgcccaccgc acgacagagg aaaatctggc cgatctgcag
     4561 gggccaggcc gcttcacgca ggttgagctg gcggcgacca cgcaccattc ttatgccatg
     4621 ccccatgaga acggccgcca gttcttcatc tgccgcgggc cgcagttctc ccttgcccag
     4681 atctggccgg gtgagaaaga attcatttga tcaccggccc ggaccttgca ccgttggcag
     4741 gctgccctcg tagaccattg gatcgatcag gcctctctgc cgggatgctt ggccgcgtaa
     4801 ttgctgctgt ggggatggca gctgcttaag gaacgcatca gcctctgcat tatctccatc
     4861 agcctttgcc tttgacgatg gctccatttt cttgagtact ccccgctcct tgtccgaata
     4921 ccagcctaag tagtgaatca ggcgctttcc cctatccggt atgtgcgctg tcacctccac
     4981 caggaattcc agcgcatcaa acacctgaaa attgcgcgac acccctccgg aaccgatcat
     5041 ccccaggccg cggaacccgc tcgcattcac ttttcccggc ccggcggatc acctgcccat
     5101 cttcggtaac cttggtcatc cgcgccagcg aaaaggaaca gcatgaaata tacgaattca
     5161 gacactgcat gcctgcatca tctgacgcag tctgcacaga gctgttgatg ctgaatcccg
     5221 agtgcggcag caccgcatcg aacgcactac ctcctcatca atcttttcct cgcgcagtaa
     5281 aaaatcaaag ccccaatccc tccaactctc tgtacatcgc tccatatcca ccctggtgat
     5341 ccgggaaaaa atccgtcccg cttgaacaat ccatcgcttt ataaattatg ctgattggct
     5401 gtgccaaccc atcagatcgc caaactcctt tctttgcctg acgatctcga tgccatgtga
     5461 tacttagttg atgaccacca tctttttggt cagaatctcc ttcggcgtcc tcatgcggta
     5521 aaagtagagc ccgggcggat ggccggctgt atgccaggta acccgatgca atccaggctg
     5581 gttcatttca ttgatgagtg tggcgacttt ttgcccgagt agattaaaga cagcgatctc
     5641 cacaggcata gcgccgtcca actggtattg aatggttgtg cggctgcgga cgggatttcc
     5701 cgcggcattg aagagcatgg tccgggaact gctcagctcc ctggcttcga tcgtggtcgt
     5761 gaggggcagg g
//
LOCUS       DAOPWU010000217         5756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13897_length_5756_cov_4.316787, whole genome shotgun sequence.
ACCESSION   DAOPWU010000217 DAOPWU010000000
VERSION     DAOPWU010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13897_length_5756_cov_4.316787"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKM23_10675"
     CDS             <1..386
                     /locus_tag="PKM23_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNY91976.1"
                     /translation="LGEFEAAEGVIEAPIRQFGSGRMGVDHLQGKPSSTSYTRLKCYE
                     GTTLIRAFPRTGRRHQLRVHFYSIGHPIAGDSRYGDKALQAIWPRLMLHAEGIEFLSP
                     AGEMVSLHAAPPSSFMELLAGLAEA"
     gene            413..1282
                     /locus_tag="PKM23_10680"
     CDS             413..1282
                     /locus_tag="PKM23_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNY91977.1"
                     /translation="MEKLRIGTCSWKYPSWEGLVYSAPGGIDYLHEYAQRYPTVEIDQ
                     WFWSLFENKVVLPKAADVENYLRAVPVDFRFTVKAPNSVTLTHPYGKVQGEAPAANPF
                     FLSPVVMGDFLARIEPLRERIALLFFQFEYLNRQKMASQGEFQQRMLEFRRRLPADLP
                     CGLEIRNSGWLNPAFFDFLLAAELRPVLIQGYWMPPVTQVYARHREQLLRHPLLMLRL
                     MGSDRQGIERDAGGSWDRILWSKEEELPAIAAMIREILSAGVEIYVNINNHYEGSAPR
                     TIARLQALLEEAN"
     gene            1345..2178
                     /locus_tag="PKM23_10685"
     CDS             1345..2178
                     /locus_tag="PKM23_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008103550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNY91978.1"
                     /translation="MKRYHWLSFLFVITLGACDKDSVSADNKPPEKPEGWQLVWSDEF
                     SVNGLPDPLKWGYQTGGSGWGNDEKQYYYADRAENARVENGMLIIEARRDNFEGHTYT
                     SARMVTRGKGDWTYGRFEIRAKLPAGRGTWPAIWMMPTESTYGSGGWPDNGEIDIMEH
                     VGYNMGTVHASIHSKMYYHKIGTQKTATTRVNDVSLKFHNYILEWDANEIRAWVDTTR
                     YFTTPNEGKGWEYWPFDKDFYLILNIAVGGSWGGAQGIDDTIFPQQMVIDYVRVYQKE
                     Q"
     gene            2227..2778
                     /locus_tag="PKM23_10690"
     CDS             2227..2778
                     /locus_tag="PKM23_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91979.1"
                     /translation="MKSLGRCFILLILLGQTLVPIPALADKASEQKQRNADVATPDAL
                     VRAAYESISGPAGRNRNWDRMRNLWMGSARIILSSNNYEGDAIYEVMTLEGFIERVSD
                     WYKQEGFYAREIASTTQRFGNIAQVWSTFEIRRGSSTSPVVYRGINSWSMVINKGRWW
                     ISQLNYDFESSRTPIPERYLKQP"
     gene            2775..4244
                     /locus_tag="PKM23_10695"
     CDS             2775..4244
                     /locus_tag="PKM23_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNY91980.1"
                     /translation="MSDHPSYHMTPEEFRRLGYRLIDWIADYEQRVADLPVLSRAEPG
                     SIRAQLPAAPPEQGEALDRVFEDLERIILPGITHWQSPNFFAYFPANTSGPSILGDLL
                     CSGLGVQGMLWATSPACTELETQLLDWMAELLALPAEFRSAGAGGGVIQDSASGAALC
                     AIIAARERKTGLVSNESGCDGRLTAYASTEAHSSIEKAIKIAGIGRKNLRLIPVDERS
                     AMRTGELETAIRTDIAAGRLPFFVSATIGTTAANGLDPLPEIGRIASQNGLWFHVDGA
                     MSGTAALCPEFRYIQEGLEFADSYCTNPHKWMFTNFDCDCMFVRDRSALIRALSILPE
                     YLRNRATESGAVIDYRDWQIPLGRRFRSLKLWFVLRHYGREGLQYHIRRHVALAQEFA
                     GWVTAHPDFELALPPPLNLVCFRHRGGEAANQAILDGINRSGKAYLSHARLAGQLTLR
                     LCVGQTRTEAEHVKAVWQLIQQAAAAWQKTPSPESRGKQ"
     gene            4241..5119
                     /locus_tag="PKM23_10700"
     CDS             4241..5119
                     /locus_tag="PKM23_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HNY91981.1"
                     /translation="MTSVQTQGMRTFSGKGGLPMLEITNRAAKALISLYGGQVLSWQP
                     LGEEQVLFISAKSWFEAGKAIRGGVPVCWPWFGPHPSGGGLPMHGFARLQEWRLQHVT
                     STQANETSAVLELEDSPATLALWPYPFQLRLQVDVGAHLRIALTMTNRDTEEVEITAA
                     LHSYFQLGEAELVHITGLDDTFYIDTLQQERTTLQQGQIRIDREINRIYLETGSSCTI
                     YDPVLARQIVVKKEGSRSTVVWNPWQERSRAFQDLGGEEYKRFVCIEAGNVRQDRILL
                     APAASHTLATEFTVIH"
     gene            5244..5435
                     /gene="rpmB"
                     /locus_tag="PKM23_10705"
     CDS             5244..5435
                     /gene="rpmB"
                     /locus_tag="PKM23_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNY91982.1"
                     /translation="MSRKCDICGKGPLVGNNVSHAHNLTKTRWMPNIRKMRIVEKGVT
                     RNAYVCTRCLRGNKVAKAL"
     gene            complement(5486..>5756)
                     /locus_tag="PKM23_10710"
     CDS             complement(5486..>5756)
                     /locus_tag="PKM23_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY91983.1"
                     /translation="EPLNRIEGVSAGRKSGREEVLQALFSIGTVEVGEALVRAVQEER
                     EWQGIYGDFAGWVHRSKAPEETLPWDFIDNGLEKGRLLRGWGEAK"
BASE COUNT         1157 a         1954 c         1598 g         1047 t
ORIGIN      
        1 ccctgggaga gttcgaggcc gcggagggag ttatcgaggc cccgatccga cagttcggct
       61 cgggcagaat gggcgtcgac cacctgcagg gcaagccctc ctccacctcc tacacccgcc
      121 tcaagtgcta tgaaggcacc accctgatcc gggcctttcc ccgcaccggc cgccgccatc
      181 agctccgcgt ccacttttac agcatcggcc accccatcgc cggcgacagc cgctacggcg
      241 acaaggcgct ccaggccatt tggccgcgcc tgatgctcca cgccgaaggc attgaattct
      301 tgagcccggc gggagaaatg gtgagcctcc atgcggcgcc gccctcctcc ttcatggagc
      361 tgctggccgg gcttgcagag gcttaaaccg acgccacgcc acggaggtaa tcatggaaaa
      421 gctgcgcatc ggcacctgca gctggaaata cccctcctgg gagggactgg tctactccgc
      481 tccggggggt attgattatc tgcacgaata cgcccaacgc tatcccaccg tcgagatcga
      541 ccagtggttc tggtcgctct tcgaaaacaa ggtggttctg cccaaggcgg ctgatgtcga
      601 gaactacctc agggccgtgc ccgtggactt tcgctttacg gtcaaggccc ccaacagcgt
      661 caccctcacc catccctatg gcaaggtgca aggggaagca ccggccgcca atcccttctt
      721 cctctcgccg gtggtgatgg gcgactttct cgctcgcatc gaacccctgc gggagcggat
      781 cgccctgctt ttcttccagt tcgaatatct caaccgccag aagatggctt cgcaggggga
      841 gtttcagcag cgcatgctgg agttcaggcg ccggcttccc gcagatctac cctgcggact
      901 ggagatccgc aacagcggct ggctcaaccc tgcctttttc gactttctcc tcgccgctga
      961 actgcggccg gtgctcatcc agggctactg gatgcccccg gtgacccagg tctatgcccg
     1021 gcaccgggag cagctcctcc gccatcccct gctgatgctg cgcctgatgg gcagcgaccg
     1081 ccaggggatc gagcgcgacg ccggcggctc ctgggaccgt atcctatggt cgaaagagga
     1141 ggagctgccc gccatcgccg ccatgatccg ggagatcctc tccgccgggg ttgagatcta
     1201 tgtcaatatc aacaatcatt atgagggttc agcgccgcgc accatcgcga ggctgcaggc
     1261 gcttctggag gaggcaaatt agaggttgcg aaaaagcgcg aatttggcta attttacaca
     1321 tcttgaacaa cccggaagga taaaatgaaa agataccact ggttgagctt tctgttcgtt
     1381 atcaccctcg gggcctgtga caaggactcg gtctcggccg acaacaagcc accggaaaaa
     1441 cccgagggct ggcagctggt ctggtccgat gaattttccg tcaatggctt gcccgatccc
     1501 ctcaaatggg ggtaccagac cggcggcagc ggctggggaa acgacgagaa gcagtattat
     1561 tacgccgatc gcgccgagaa cgcccgggtc gaaaacggca tgctcatcat cgaggcacgc
     1621 cgggataact ttgagggcca cacctacacc tcagcgcgca tggtcaccag aggcaagggc
     1681 gactggacct atggccgctt cgaaatccgt gccaagcttc cggccggccg cggcacctgg
     1741 cccgccatct ggatgatgcc caccgaatcg acctacggca gcggcggctg gcccgacaac
     1801 ggcgagatcg atatcatgga acatgtcggc tacaacatgg ggaccgtcca tgcctccatc
     1861 cactccaaaa tgtactatca taaaatcggc acccagaaaa ccgccaccac cagggtcaat
     1921 gacgtctccc tcaagtttca caattacatc ctcgaatggg atgcgaacga aatcagggcc
     1981 tgggtcgaca ccacccgcta tttcaccacc cccaacgagg gcaagggctg ggaatactgg
     2041 cccttcgaca aggatttcta cctcatcctc aacattgccg tcggcggcag ctggggggga
     2101 gcgcagggca tcgatgacac catcttcccc cagcagatgg tcatcgacta tgtccgcgtc
     2161 taccaaaagg agcaataggc tcccctccgg gagagcctgg acacccaact gtacaaggtg
     2221 gaggctatga aatcactcgg caggtgtttt atattgctga ttcttctcgg ccagaccctg
     2281 gtgccaattc ccgccctggc ggacaaggcc agcgaacaga aacagcgaaa tgcggatgtc
     2341 gccactccgg acgccctggt gcgcgcggcc tatgagtcga tctcgggccc ggccggccgc
     2401 aatcgcaact gggaccgcat gcgcaacctc tggatgggca gcgcccggat tatcctctct
     2461 tcgaacaact atgagggaga tgctatttat gaggtcatga ccctcgaggg ctttatcgag
     2521 cgggtctccg attggtacaa gcaggaggga ttctacgcgc gcgagatcgc ctcgaccacc
     2581 cagcgcttcg gaaatattgc ccaggtctgg agcacctttg aaatccgcag gggatcctcc
     2641 accagtccgg ttgtctatcg cggtatcaac agctggtcga tggtcatcaa taagggccgc
     2701 tggtggattt cgcagctcaa ctacgacttt gaaagcagcc gaactcccat cccggagcgt
     2761 tatctcaagc agccatgagc gatcacccct cctatcacat gacgccggag gagttccgcc
     2821 ggctcggcta ccgtctcatc gactggatcg ccgactatga gcagcgggtc gccgacctgc
     2881 cggtcctgtc gcgcgccgaa cccggcagca tccgcgccca gctccccgcc gcaccgcccg
     2941 aacagggcga agccctcgac cgggtctttg aggatctcga gcggatcatc ctccccggca
     3001 taacccactg gcaatcgccc aactttttcg cctatttccc ggccaacacc tccggccctt
     3061 ccatcctcgg cgacctgctc tgctcaggcc tcggcgtcca gggcatgctc tgggccacca
     3121 gccccgcctg cactgaactg gaaacccagc tgctggactg gatggctgag ctgctcgccc
     3181 tccccgcgga attcaggtct gcgggcgccg gcggcggggt gattcaggac tccgcctcgg
     3241 gcgcagccct ctgcgccatc atcgccgccc gcgaacgcaa gaccggactg gtcagcaacg
     3301 agtcaggctg cgacggccgc ctgaccgcct acgcctcgac cgaagcccac tcctcaatcg
     3361 aaaaggcgat caaaatcgcc ggcatcggcc gcaaaaacct gcgcctcatt ccggtcgacg
     3421 agcgctccgc catgcgcacc ggggaactcg aaaccgccat ccgcactgat atcgcagccg
     3481 gccgcctccc cttctttgtg agcgccacta tcggcaccac cgccgccaac ggcctcgatc
     3541 ccctccccga aatcggccgc atcgcctccc aaaacgggct ctggtttcat gtcgacgggg
     3601 cgatgagcgg caccgccgcc ctctgccccg aattccgcta tatccaggag ggactggagt
     3661 tcgccgacag ctactgcacc aatccgcaca agtggatgtt caccaatttt gactgcgact
     3721 gcatgttcgt ccgcgaccgt agcgccctca tccgcgccct gtccattctg ccggaatatc
     3781 tgcgcaaccg ggcgaccgag tcgggcgctg tgatcgatta ccgcgactgg cagattccgc
     3841 tcggccgccg cttccgcagc ctcaagctct ggttcgtctt gcgccactac ggccgggagg
     3901 gactgcagta ccacatccgc cgccatgtcg cactggccca ggagttcgcc ggctgggtca
     3961 ccgcccatcc cgatttcgaa ctcgccctcc cgccgccgct caatctggtc tgcttccgcc
     4021 accgcggcgg tgaggccgcc aaccaggcca tcctcgatgg gatcaaccgc agcggcaagg
     4081 cctatctctc ccacgcccgc ctggcgggcc agctgaccct gcgcctctgc gtcggccaga
     4141 cccgcaccga agccgaacat gtgaaggcgg tctggcagtt gatccagcaa gcggccgcag
     4201 catggcaaaa aactccttca cccgaaagca gaggaaaaca atgacgtccg tccaaacgca
     4261 ggggatgcgc accttctcgg gcaagggtgg tctgcccatg ctggagatca caaacagagc
     4321 cgccaaagcg ctgatcagcc tttacggagg gcaggtgctc tcatggcagc ccctgggtga
     4381 agaacaggtg ctctttatca gcgcaaagag ctggttcgag gcgggcaagg ccatccgcgg
     4441 aggagtcccg gtctgctggc cctggttcgg cccccacccc tccggcggtg ggctgccgat
     4501 gcacggcttc gcccgcctcc aggaatggcg tctccagcat gtgacctcca cccaggccaa
     4561 cgaaaccagc gccgtgctgg aactcgagga ttctcccgcc accctcgccc tctggccgta
     4621 ccccttccag cttcgcctcc aggtcgacgt cggcgcccat ctccgcatcg ccctgaccat
     4681 gaccaaccgc gacacagagg aggtcgagat caccgcagcc cttcattcct atttccagct
     4741 cggggaggcg gaactggtac acatcacggg gctggacgac accttttaca tcgacaccct
     4801 ccagcaggag cgcaccaccc tgcagcaggg gcagatcagg attgaccggg agatcaaccg
     4861 catctatctc gaaaccggca gcagctgcac catttatgat ccggtgctgg cacggcagat
     4921 tgtggttaag aaggagggca gccgctcaac cgtggtctgg aatccgtggc aggagcgcag
     4981 ccgcgccttc caggacctgg gcggggagga gtacaagagg tttgtctgca tcgaggccgg
     5041 gaatgtgcgt caggatcgca tcctgctcgc acccgcggcc agccataccc tggcgacgga
     5101 attcaccgtc atccattagc ctggagtgca acgagagcag gaaaacctcc attttatgct
     5161 tgcttttttg atttattatt tctattttaa tcagcttggt aggaaaatat tcaatacagg
     5221 ataaatgagc aggagttttt ccaatgtcga gaaaatgtga tatatgcggc aagggcccgc
     5281 tagtcggcaa caatgtcagc cacgcccaca acctgaccaa gacgcgctgg atgcccaaca
     5341 tccgcaagat gcgcatcgtc gaaaagggcg tgacgcgtaa tgcctacgtc tgcacccgct
     5401 gcctccgcgg caataaagtg gccaaggccc tctaacctca gccgctgcgc caaacaactc
     5461 aagccgggtc ccgtacccgg cttttttatt tcgcctcccc ccacccgcgc agcagccgcc
     5521 ctttttccag tccgttatcg atgaaatccc agggcagcgt ctcctctggg gccttgctgc
     5581 gatggaccca gccggcaaaa tctccataga tcccctgcca ctcccgctct tcctgcaccg
     5641 cccgcaccag ggcctcgccg acctccacag tgccaatcga gaagagggcc tggagcacct
     5701 cctcacgtcc gcttttgcgg ccggcgctca ccccttcgat gcgattgaga ggctcg
//
LOCUS       DAOPWU010000218         5744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_13936_length_5744_cov_5.390754, whole genome shotgun sequence.
ACCESSION   DAOPWU010000218 DAOPWU010000000
VERSION     DAOPWU010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13936_length_5744_cov_5.390754"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="PKM23_10715"
     CDS             complement(<1..1691)
                     /locus_tag="PKM23_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNY91984.1"
                     /translation="MKVARISLFAALLALTASLTYAQPAVEVRVQNLLDQMTLEEKVG
                     QMTQITLEVVSVGEGPGIREPLMLDRKKLREIILDRGVGALFNVGTHAHSLESWNEII
                     SEIQDVAREGRLKIPVLYGIDAIHGATYTRGATLFPQSIGMASTWNPDLLRLASEITA
                     VEVRASGIPWNFNPVLDSGRQPLWPRLWETFGEDALLTTAMGVAYVKGQEGDANDISA
                     PDRVASCLKHYIGYGHPVSGKDRTPAWVPDHYLRQYILPPYIAAVAAGAHTVMVNSGD
                     INGVPVHINRYLLTDLLRGELGFKGLIDSDYADIAKLYQNHRVAADNKDAVRMAVMAG
                     IDMVMVPADFSFTDDLIALVREGKVPMSRIDEACGRILKLKYQLGLFDNPYPDKSLKA
                     RFATPEAAVINLRAAQESLVLLKNEGGILPLKKSAKILVTGPTANRLSVLNGGWTYTW
                     QGNREDLLPKDKLTILKAIAAEAGANRVSFSLGSTYDSEADIAAAVKAAKKADVVVAC
                     LGEEPYCETPGNIGDLTLPQSQLDLVRELQKSGKPVVLVLVEGRPRIIRPIVEKSA"
     gene            complement(1807..4785)
                     /locus_tag="PKM23_10720"
     CDS             complement(1807..4785)
                     /locus_tag="PKM23_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNY91985.1"
                     /translation="MNPCLRAAALLLIWFTAAAAQNFSGGFNFVLPARDSTRQQFLPA
                     FPAQPIAAGAFVGINAEGHFAVAGERIRFWGTNCVTDGAFPPSDKAPFLAARLCKMGF
                     NLVRFHHMDNPWSDHSLFESGSDTRHLNPVMLGRLDKFITELKKNGIYLNINLHVSRT
                     FNSRDGVADADSITDYGKAVSYFDRDLWPLYKEFARQLLTHRNPYTGLALVEEPAMAM
                     VEITNENSLYRWWRDGLLRPFTGGGRLTLRHSRMLDLQWLAFLQQKYATTARLRSAWL
                     PGSRDGRGGELIKDGGFEKGELARNWIMEQHETARATMALAAVQPFSGALAAKVSVTA
                     VTGTGWHIQWKQTGFTLQKDSLYTVSFAARTEVPCAISAAVMRDTSPWTGYGSGSFSL
                     SPAWQRFSFTFKAAETCANQGRLSFHLGEQTGNFWFDEISIQPATISGLLEDESLEAG
                     NIRRTEYNQSVSFSAARVSDMSAFYIRLQEDFFSEMNRFLKQELGVRVPVVGTNWNVG
                     PADILVQNQLDYIDNHAYWDHPDFPKTPWSVTDWTIANTPMVLETAGGAIAGVINATA
                     VAGKPFTISEYNHPFPNRYQSEGPLFLCSYAALADADAVMLFDNGGSDTSYETDFIDG
                     YFGIHRNTAMMALMPACAAAFRQGWIAPAQQTIAIALSPADVLTLPLNDTGGWQGPGI
                     VPRLLALQHRVRYTNFAAAAPFDAAALPAPPAPPFLSDTDELLWNPAGLFTTVTPRYL
                     AFTGLLDRFPETGMGPLRLHSASGFASCSWASLDGAPLTEARYSLFTITTRTQNSGMI
                     WEGTRTIHNSWGKAPTWIEPVRLGLTLTLRSDSIRVWPLDGSGARRGSGRKIVPASAG
                     TFNLILDQSQDQTVWYGIEQLGALSGVSDRGEAVQPAALLLKQSHPNPFRVSTQGHTE
                     ITFRLGQPGEIRLVIYDLLGRQVRELAAGPAGAGETSMRWDGRDAGARPVAQGLYFYL
                     LEYAEAGMRQHTAGKLLLLP"
     gene            complement(4782..>5744)
                     /locus_tag="PKM23_10725"
     CDS             complement(4782..>5744)
                     /locus_tag="PKM23_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNY91986.1"
                     /translation="RSQGCHDWYLSHYASLNLFTSDLGWGSLAPLAHLTYLFDPDPRA
                     DQTVRTRLRQALHAYAGALLQQQAQNGFGYLLKPGEYNWGSTSRALNRALLLIMASES
                     GTGSAWRQAALDQIHYMLGANPHNISFVTGVGTMSMKNPHHRPSWADNVPAPVPGLLS
                     GGANQYLDDTILKAAFTSSTPPALCWIDDANSYASNEIAINWNAPLVFLAGYFNPEGS
                     GSGVGDAPQAVPSRFRLGQNYPNPFNDATVIPFHLETAGEATLQIYNIRGELVDEQLL
                     GRLESGEQRHHWQARGPSGLYYYRLQVASDENLLTLTGKMVVVR"
BASE COUNT         1078 a         1642 c         1941 g         1083 t
ORIGIN      
        1 gcggattttt ccacgatcgg gcggatgatg cgcggccgcc cctccaccag caccaggacg
       61 accggcttgc cgctcttttg cagctcccgg accaggtcga gctgggattg aggcagggtc
      121 agatcgccga tgttgcccgg ggtttcgcaa tagggttcct caccgaggca ggcgaccacc
      181 acgtcggcct ttttggccgc cttgacggcg gccgcaatat cagcctcgct gtcgtaggtg
      241 ctgcccaggc tgaaactcac cctgttggcg ccggcctcgg ccgcgatcgc cttgaggatg
      301 gtgagcttgt ccttgggaag aagatcctcg cggttgccct gccaggtata ggtccatccc
      361 ccgttcagga ctgagaggcg gttggccgtt ggcccggtca ccaggatttt ggcgctcttt
      421 ttaaggggca gaattccgcc ctcattcttg agcaggacca gggattcctg tgccgcccgc
      481 agattgataa cggccgcctc aggggtggcg aagcgcgctt tcagggattt gtcgggataa
      541 ggattatcaa aaaggccgag ctggtacttg agcttgagga tgcggccgca ggcctcgtcg
      601 atgcggctca tcggcacctt gccctcccgg accagggcga tcaggtcatc ggtaaagcta
      661 aagtcggccg ggaccatgac catgtcgatg ccggccataa ccgccatgcg cacggcatcc
      721 ttgttgtcgg cggcgacgcg atggttctgg taaagcttgg caatatcggc ataatcactg
      781 tcgatcaaac ccttgaaacc cagttccccg cgcagcaggt cggtgagcag atagcggttg
      841 atgtggaccg ggacgccgtt aatgtcgcca gagttgacca tcacggtatg ggcgccggct
      901 gcaacggccg caatgtaggg cggcaggata tactggcgga ggtagtggtc gggcacccag
      961 gccggggtgc gatccttgcc ggagacggga tggccgtagc cgatatagtg tttgaggcag
     1021 gaggccaccc ggtcgggcgc ggagatatcg ttggcatccc cctcctgtcc cttgacgtag
     1081 gcgacgccca tggcggtggt gagcagggcg tcctcgccga aggtttccca gaggcgcggc
     1141 cagagcggct gccggccgga gtcgaggacg gggttgaaat tccacgggat gccggaggcg
     1201 cgcacctcga cggcggtgat ctcgctggcc aggcgcagca gatcgggatt ccaggtcgag
     1261 gccatgccga tggactgggg aaagagggtc gccccgcggg tataggtagc gccatggatg
     1321 gcatcgatgc cgtagaggac cggaattttc agccgccctt cgcgcgccac atcctgaatc
     1381 tcgctgatga tctcgttcca gctttcgagg ctgtgggcat gggtgccgac gttgaaaagg
     1441 gctcccacgc cgcggtccag aatgatttcg cgaagtttct tgcggtcgag catgaggggt
     1501 tcgcggatgc cgggaccctc cccgacgctg acgacttcga gggtgatctg agtcatttgc
     1561 ccgaccttct cttccagggt catctggtcg aggaggttct gcacccttac ctcgacggcg
     1621 ggctgggcat aggtgaggct ggccgtcagc gccagcagag cggcgaacag agagatacga
     1681 gcaactttca tgatcatatt ccttgcaatt agataccggc ctgatttttc tataatatac
     1741 atataattga gcgaaaatgc ataaattttg agctggatca caatattttg ccgttatcgg
     1801 cgcgcctcaa ggcaggagca gcagcttgcc ggcggtatgc tggcgcatcc cggcctctgc
     1861 atactcgagc agatagaaat agaggccctg cgcaaccggg cgggcgccgg cgtcgcggcc
     1921 atcccagcgc atgctggttt caccggcacc ggcagggccg gctgccagtt cgcgcacctg
     1981 acgcccgagc agatcataga tcacgaggcg gatctcgccc ggctgcccca gccggaaagt
     2041 gatctcggtg tgtccctgtg tggagactcg aaaaggattg ggatggctct gcttgagcag
     2101 cagcgcagcg ggctgcaccg cctctcccct gtcggacaca ccgctgagtg ccccgagctg
     2161 ttcgatgccg taccagaccg tctggtcctg gctctggtcg aggatcaggt tgaaggtccc
     2221 tgccgaagcg ggcacgattt tgcggccgct cccccgcctg gctccggagc catccagcgg
     2281 ccagacgcgg atcgagtcgg agcgcaaggt caaggtgagg ccaagtcgca ccggttcaat
     2341 ccaggtaggt gccttacccc agctgttgtg gatggtccgg gttccctccc aaatcatgcc
     2401 gctgttctgc gtccgggtgg tgatggtgaa cagggaatag cgcgcctcgg tcagcggcgc
     2461 cccgtcgagc gacgcccaac tgcacgaagc aaaaccgctc gcgctgtgca gacgcagcgg
     2521 ccccatcccg gtctcgggga aacggtccag cagaccggtg aaggccagat agcgcggcgt
     2581 gaccgtcgtg aagaggccgg ccggattcca gagcagctcg tcggtatcgc tcagaaaagg
     2641 cggtgccggc ggtgcaggca gcgctgccgc atcaaagggg gcagccgcgg caaagttcgt
     2701 atagcgcact cgatgctgca gggcgagcag acgcggtacg atcccggggc cctgccagcc
     2761 gccggtgtcg ttcagcggca gggtcagcac atccgcagga ctcagggcga tggcaatcgt
     2821 ctgctgcgcc ggtgcgatcc acccctgccg gaaagcggca gcacaggcag gcatcagcgc
     2881 catcatcgct gtattgcggt gaatgccgaa ataaccgtcg atgaaatcgg tttcgtagct
     2941 ggtgtcgctg ccgccgttgt cgaagagcat caccgcatcg gcatcggcca gggcggcata
     3001 gctgcacaga aagagcggcc cttcgctctg gtagcgattg gggaaggggt gattgtactc
     3061 gctgatagta aagggcttgc ccgctaccgc ggtggcattg atcacgccgg cgatcgcccc
     3121 gcctgcagtc tcgagcacca tcggggtgtt ggcgatggtc cagtccgtga cggaccaggg
     3181 ggttttggga aaatcaggat ggtcccagta ggcatgattg tcaatatagt cgagctgatt
     3241 ctggaccagg atgtcggcgg ggccgacatt ccagttggtg ccgacgacgg gcacgcgcac
     3301 acccagttcc tgcttgagaa agcggttcat ctcggagaaa aaatcctcct gcaggcggat
     3361 ataaaaagcg ctcatgtcgg agacccgcgc cgcggaaaag gagacgcttt gattatactc
     3421 ggtgcggcgg atgttgcccg cctcgagcga ttcatcctcg agcaggccgg aaatcgtggc
     3481 cggctggatt gaaatctcat cgaaccagaa gttgccggtc tgttcgccga gatgaaagga
     3541 gagcctgccc tgattggcgc aggtttccgc agccttgaag gtgaaagaga agcgctgcca
     3601 tgcgggagag agggaaaaac tgccgctgcc gtagcctgtc cagggtgagg tgtcgcgcat
     3661 caccgctgcg ctgatggcgc atgggacctc cgtccgggct gcgaaggaga cggtgtagag
     3721 cgagtctttt tgcagggtaa atccggtctg cttccactgg atgtgccagc cggtgccggt
     3781 tacggccgtg actgacacct tggccgcaag tgcaccgctg aagggctgca cggcggccag
     3841 ggccatggtg gcgcgggcag tctcgtgctg ctccatgatc cagtttcgag cgagctcgcc
     3901 cttttcaaag ccgccatcct tgatgagttc gccgccacgt ccgtcccggc ttcccggcag
     3961 ccaggcgctg cgcagccggg cagtggtggc gtatttctgc tgcagaaatg ccagccactg
     4021 caggtcgagc atgcgggagt ggcgcagagt gagccgcccg ccgccggtga agggccggag
     4081 caggccgtcg cgccaccagc ggtaaagtga gttctcgttg gtgatttcga ccatggccat
     4141 cgccggctcc tctaccagag ccaggccggt ataaggatta cgatgggtga gcagctggcg
     4201 ggcaaactct ttatagaggg gccagagatc gcggtcgaaa taactgaccg ccttgccgta
     4261 atcggtgatc gaatctgcgt cggcgactcc gtcacggctg ttgaaggtgc ggctgacgtg
     4321 caaattgata ttcagataga tgccattctt tttcagttcc gtgataaatt tgtccagtcg
     4381 ccccagcatg accggattga gatggcgggt gtcggagccg ctttcgaaga ggctgtggtc
     4441 gctccaggga ttatccatgt ggtggaagcg gacaagatta aaacccatct tgcacagccg
     4501 cgccgccagg aaaggtgcct tgtcggaggg gggaaaggcc ccatcggtga cgcagttggt
     4561 gccccagaaa cggatgcgtt ctccggccac ggcaaagtgg ccctccgcgt tgatgccgac
     4621 aaaagcgccg gcggcgatgg gctgggccgg aaaggcaggc aggaactgct gacgggtgct
     4681 gtcccgcgcc ggcaggacaa aattgaaacc ccctgaaaag ttttgtgctg ctgcagcggt
     4741 aaaccagatc aggagcagcg cggcggcgcg gaggcatgga ttcatctcac caccaccatt
     4801 ttcccggtga gggtgaggag gttctcatcc gatgccacct gcaggcggta gtaataaagg
     4861 ccgctgggcc ctcgcgcctg ccaatggtga cgctgctctc cggattcgag gcggccgagg
     4921 agctgctcgt cgaccagctc gccgcggatg ttgtagatct gcagggtcgc ctcgcccgcg
     4981 gtttcgagat ggaaggggat gacggtggcg tcattgaagg ggttgggata gttctgcccc
     5041 agccggaagc ggctgggaac cgcttgcggg gcgtccccga ccccgctgcc tgagccttca
     5101 ggattgaaat agccggccag gaagaccagg ggagcgttcc agttgatggc gatttcgttg
     5161 gaggcatagc tgttggcgtc atcgatccag cacagcgctg gcggcgtcga ggaggtgaag
     5221 gccgccttga ggatagtatc atccagatac tgattggccc cgcccgacag cagtccgggc
     5281 accggcgcgg gcacgttgtc cgcccaggag gggcgatgat ggggattttt catgctcatc
     5341 gtgccgacgc ccgtgacgaa agagatgttg tgcgggttgg ccccgagcat gtaatggatc
     5401 tgatccaggg ctgcctggcg ccaggcgctg ccagtgcccg attcgctggc catgatcagc
     5461 agcagggcgc gattgagggc gcgggaggtg gagccccagt tgtactcgcc gggtttgagc
     5521 aggtagccaa agccgttctg cgcctgctgc tgcagcagcg cgccggcata ggcgtgcagg
     5581 gcttgccgca gccgggtgcg cacggtctga tccgcgcgcg gatcgggatc gaacagatag
     5641 gtcagatggg cgaggggcgc cagtgagccc cagcccagat cagaggtgaa gagattgagc
     5701 gaggcgtaat ggctgagata ccagtcatga cagccctggc tgcg
//
LOCUS       DAOPWU010000219         5723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14007_length_5723_cov_4.400494, whole genome shotgun sequence.
ACCESSION   DAOPWU010000219 DAOPWU010000000
VERSION     DAOPWU010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14007_length_5723_cov_4.400494"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKM23_10730"
     CDS             complement(<1..683)
                     /locus_tag="PKM23_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNY91987.1"
                     /translation="MKRLLAFLVIVPALSFLISACEDEPTAPEAPAVSKAIYVLNSAA
                     TSISVIDLVENKVYNNVQTVGTWPNQLVYRNGYLYCVNSGSNNIMKFDVKTWAAKPPV
                     DLGSGKNPMNMVFYNNQFAYVACSMSNEVLRVDMTSNTVVKTIKAGVGTTGIEIANNK
                     VYASNTGYAGWGNPYLPGTVTVINAATGDSLKTITVGTNPQSIALAKDGKLHVSCTGD
                     YGATPGVVSV"
     gene            complement(698..2908)
                     /locus_tag="PKM23_10735"
     CDS             complement(698..2908)
                     /locus_tag="PKM23_10735"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNY91988.1"
                     /translation="MKKSGVIPVLFALILALPAPARPDHGQITGTVSSAETNEPLPAA
                     NVYLKNTHVGCVTDTQGGFTIDQIPAGEYILVADYVGYAEVSRVVKVTEGSSLKLQLK
                     LKPAPYKFNDPITVTATRGVSLASEVPASVNIVNAREMEKRLSVNAAEALQSIQGITI
                     KDYGGLGGVKSISLRGSSSEQVLVLLDGQRINNPQTGQVDLGQIPLEGIKQIEVVRGG
                     NSALYGADAVGGVINLITDDGSHSRPGFSGSVKMTNASFRTWSMETFGKYLYKQWQLQ
                     SSVDILSARGDFPFTDNYGLSRTRKNADVLSKDFYFKVVRNFGRIESNRKLDVSFKWY
                     DAERGAPGTIEPYYYHARQWDSNRLLNLLFTSKVFNYLNDLRIQAYHYDNWNRYENDE
                     SLTPIRSEFRAKTAGSEIQMESILNTHCSLTYGLGLRLDYMTDYEADTDRQRTNFYLF
                     LLNESTIRFERKWLKSIVFVPSLRYDKNSDYRDNLAPKTGVVINFGENFKTAVKGNIG
                     CSYRAPTFNDLYWPEDAWTKGNSALKPEHGYDWDAGLRLQIPWLNGITLESTFFQNRM
                     VDLIIWAQNDQALWMPENVDQSLIRGVENAFSFKPFHKNWQLSGNYTWLDARNLSGSS
                     LEKDKLLVYRPKHTLNLFSDFSIRAFSVNYQFQFTSRRFADKTNFWNNSLEPYTLSTL
                     GCSYNWNLDHLGLNASLQVRNLFDEEYRTIKNMPMPGREYRCAVKFTLNSPSTN"
     gene            complement(2919..4790)
                     /locus_tag="PKM23_10740"
     CDS             complement(2919..4790)
                     /locus_tag="PKM23_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91989.1"
                     /translation="MMRKSVCLWLGILIISAVTGYAQAQDSKEKEKSASEVVLTQEDF
                     VKNNCENVGDALQTITGVYINAEGEISLRDVSASKVVVIMDGQRLNTPGSTGVNIASL
                     QIQNVEKIELLRGGRSAQYGADAVGGVILITTKVKEDNQKALNLNAKATYGSYNRQIY
                     TLSESYGRKNLNSFVSYQRETWDGDFTYLDYYDVKKNLINNKQSSHNIFGKVGYNLDE
                     NQQLSTSANWYYADNGTPGMIDNLTPNARLRLDNKSYNLTYNNKKTFRDFSLKAQSYY
                     LDMETKFDNPDAFGGAVHSDHNNYALGLELSQSGSLGANFALDYGYSYRNDRIKSTDV
                     GKKHRDTNSGHVALTGHAELPAWAYLTLFEASIAARHDAPTDFRAATSPRFSASFSHQ
                     GDLSLSLMGHVAKSYKAPSFNDLYWPQDAFDVGNPDLVPEDGFNYDIGLSATYSTLSI
                     TTNYFRNDIDNLIPWEQDPALNNLWTPKNISKSNTFGIETSVNLDLLNRLISLNSEYT
                     FMKALDKGPDPSRHDRYITYRPKNKLDLTGTLRIWDIEWNAIYHYKGLRFTNAANTKW
                     LPAFHTVDTNLTYKFNFVNLQWMTIFELSNLTDESFMQVLGTAEPGRMYKISLGANF"
     gene            complement(4795..>5723)
                     /locus_tag="PKM23_10745"
     CDS             complement(4795..>5723)
                     /locus_tag="PKM23_10745"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNY91990.1"
                     /translation="SMMSPETIKKIFGYIDQFLPAPPGHIIHDGMAAGAVTDDTQQTL
                     LFADSIIARQGVDPEDIAQRLIQWGEQIRAFESMAVGPSSLRALYAIKSGKSIHEAGR
                     AGDTNGAVMRIAPVGILGFGDRDKTMDLVSRACIPTHNTNIAIAGASAIAMAIGQALK
                     GEQDVDSLIQSAIGIIEPAMQLGNIWYSASILDRANLALDIVKRTKDRAEALEKLYRV
                     IGAGVQISETVPTCLALTRLVDGDPVQGVIAAANLGGDCDTIGAITGAICGAIRGIDA
                     FPGDWVQLLSATNHIDFDWYTGKLYQTITGKE"
BASE COUNT         1209 a         1512 c         1640 g         1362 t
ORIGIN      
        1 acactgacca ctccgggggt tgcgccatag tcacccgtgc aggagacatg cagcttgcca
       61 tccttggcca gggcaatcga ctggggattg gtgccgactg tgatcgtttt cagggaatcg
      121 ccagtggccg cattgatcac cgtgacggta ccgggcaggt agggattgcc ccagccggcg
      181 tagccggtat tgcttgcata gaccttgtta ttggcaattt cgatgccggt tgtaccgacc
      241 ccggccttga tggttttgac cacggtattg ctggtcatat ccacacgcag gacttcattg
      301 gacatggagc aggccacata ggcgaactgg ttgttataaa aaaccatgtt catcgggttc
      361 ttgccgctgc cgaggtccac gggtggtttt gctgcccagg tcttgacatc gaacttcatg
      421 atgttgttgg agccggaatt gacacagtag agatagccgt tccggtaaac cagctgattg
      481 ggccaggtcc ccacggtctg cacattgtta tagaccttgt tctccaccag atcgatcacc
      541 gagatgctgg tggcggcgga attgagcaca tagatcgcct tggaaacagc cggtgcctcc
      601 ggagcggtcg gttcatcctc gcaggcggaa atcaggaatg ataacgccgg cacgatgacc
      661 agaaaagcca gtagtcgttt catagcaact ccttttgtta gttggttgac ggactgttta
      721 gcgtgaattt taccgcacaa cgatattcgc gccccggcat gggcatattt ttgatcgtcc
      781 ggtattcctc atcaaaaaga ttgcgcacct gcagggaggc attcaaaccc aggtgatcca
      841 ggttccagtt atatgaacag cccagggtcg acagggtgta aggctcgagt gagttattcc
      901 agaagttggt cttgtcggcg aaccgccggc tggtgaactg aaactgataa ttcacggaaa
      961 aggcgcggat cgaaaagtcg gagaagagat tcagggtatg ctttggccgg tagaccagga
     1021 gcttgtcctt ttccagcgag ctgccggaga ggttgcgcgc atccagccag gtgtagttgc
     1081 cgctgagctg ccagtttttg tggaagggtt tgaacgaaaa ggcattttcc accccgcgga
     1141 tcaacgactg gtccacgttc tctggcatcc acagcgcctg gtcattttgc gcccagatga
     1201 tcaggtcaac catgcggttc tggaaaaagg tcgattccag ggttatgccg ttcagccagg
     1261 ggatctgcag cctcaggccg gcatcccagt catagccgtg ttccggtttc agtgcgctgt
     1321 tgccctttgt ccaggcatct tccggccagt agaggtcatt gaaggtcggg gcgcgatagc
     1381 tgcagccgat gttgcccttg acggcagtct tgaagttttc gccgaaattg atgaccacac
     1441 ccgttttggg cgcaagattg tcccggtaat ccgaattctt gtcataacgc agcgaaggga
     1501 cgaatacgat tgacttgagc cacttccttt caaatcggat cgtgctctcg ttgagcagaa
     1561 agagataaaa attggtccgc tgccggtccg tatccgcttc ataatcggtc atatagtcca
     1621 gccggagacc aagaccatag gtcaatgaac agtgggtgtt gagaatactc tccatctgga
     1681 tttccgagcc ggcagttttg gcacggaatt ccgagcggat tggcgtcagg ctttcatcat
     1741 tttcgtatct gttccagttg tcataatgat acgcctggat gcgcaggtca ttcaggtaat
     1801 tgaacacctt gctggtgaag agcaggttga ggaggcggtt gctgtcccac tgacgggcat
     1861 gataatagta gggttcgatc gtaccgggcg ccccccgttc ggcatcgtac catttaaagc
     1921 tcacatcgag tttgcggttg gattcgatgc gcccgaaatt ccgtacgacc ttgaaataaa
     1981 aatccttcga aagcacatcc gcatttttcc gcgtccggga gaggccgtaa ttatccgtaa
     2041 acggaaaatc tcccctggcg gaaaggatgt caacggagga ttgcagctgc cactgcttgt
     2101 agagatattt gccaaaggtc tccatggacc aggtccggaa ggaggcgttg gtcatcttta
     2161 cagagccgga gaaaccgggc cggctgtggc tgccgtcatc ggtgatcaga ttgatgacgc
     2221 cgccgacggc atccgcgccg tagagcgctg aattaccgcc ccggacgacc tcgatctgct
     2281 tgatgccctc aagcgggatc tggcccagat cgacctgacc ggtctgcggg ttattgatgc
     2341 gctgtccatc gagcagcacc agcacctgct cggagctcga gccgcgcagg gagatcgatt
     2401 tcacgccacc caggccgcca tagtccttga tggtaatgcc ctggatgctt tgcagcgcct
     2461 cggccgcatt gaccgacaag cgtttctcca tctcccgggc attgacgata ttcacggagg
     2521 ccggtacttc cgaggccagg gagaccccgc gcgtcgccgt cacggtaatc ggatcattga
     2581 acttgtaagg cgccggcttt agtttgagct ggagtttgag actcgatccc tctgtgacct
     2641 tgacgactct cgatacctcc gcatagccca catagtccgc aaccaggatg tactcgcctg
     2701 cggggatctg gtcgattgta aaaccgccct gggtgtcggt cacgcagccg acatgggtgt
     2761 ttttcagata gacattggct gcaggcaagg gctcgttggt ctccgcgctg gacaccgttc
     2821 ccgtaatctg cccatgatcc ggcctcgccg gtgccggcag ggccaggatc agggcgaaga
     2881 gaacagggat tactccggat tttttcatac gtccttttct aaaaatttgc gcccaaagag
     2941 atcttgtaca tccggccagg ctcagccgtg ccgagcacct gcataaaact ttcatcggtc
     3001 aggttgctca actcaaaaat agtcatccat tgtaaattaa caaaattaaa tttataggtc
     3061 aggttcgtgt cgacggtgtg aaaagcgggc agccatttgg tattggccgc gttggtgaaa
     3121 cgcaagcctt tgtagtgata gattgcattc cattcaatat cccagatccg cagggtcccg
     3181 gtgagatcca gtttgttttt gggacgatag gtgatatagc ggtcatgccg gctgggatcc
     3241 gggcctttat ccagggcctt cataaaggtg tattcggaat tcagcgagat caggcggttc
     3301 aacagatcca gattcaccga ggtttcgatg ccgaaggtat tcgatttcga gatattcttg
     3361 ggcgtccaga ggttgttcag cgcggggtcc tgctcccatg gaatcagatt gtcgatatca
     3421 tttctgaaat agttggtggt gatggagagc gtggagtagg tggcggagag cccgatgtca
     3481 taattaaagc catcctccgg aaccagatcc ggatttccca cgtcaaaggc atcctgcggc
     3541 cagtagaggt cgttgaacga cggcgccttg tatgatttgg caacgtgccc catcagggag
     3601 agtgaaagat cgccctggtg tgaaaaagag gcgctgaatc tcggactggt cgcggcgcga
     3661 aagtccgtcg gcgcgtcatg ccgtgccgcg atcgaggctt caaacagggt cagatacgcc
     3721 cacgccggaa gctcggcatg cccggtgagg gccacatgcc cgctgttggt gtcgcgatgc
     3781 tttttaccca catccgtgct cttgatgcgg tcattgcggt aggagtagcc atagtccagg
     3841 gcaaaattgg cgcccagtga accggactgg gagagttcga gtcccagcgc gtagttatta
     3901 tgatcactgt gcacggcccc gccgaaggca tccggattgt cgaacttggt ctccatatcg
     3961 aggtaataac tctgcgcttt cagtgaaaaa tccctgaagg tcttcttgtt gttataggtc
     4021 agattatagg atttgttgtc caggcgcaaa cgggcattgg gcgtcagatt gtcgatcatg
     4081 ccgggggtgc cgttatccgc ataataccag ttcgccgatg tgctgagctg ctgattttca
     4141 tcgagattgt agcccacctt gccgaagata ttatgggagg actgcttgtt gttgatcagg
     4201 tttttcttga cgtcataata gtcgagatag gtaaaatcgc catcccaggt ctcccgctgg
     4261 tacgagacaa aggagttgag atttttacgg ccatacgatt cagacagggt atagatttgc
     4321 cggttgtatg agccgtaggt tgccttggcg ttgaggttca gggccttctg gttatcctcc
     4381 ttgaccttgg tggtgatgag aatcaccccc ccgacggcgt ccgcgccata ttgggcggaa
     4441 cgcccgccgc gcagcagctc gatcttttcc acattctgga tctgcaggga ggcgatgttc
     4501 acgcccgtcg agcccggagt gttcagccgc tgcccatcca tgatgacgac cacttttgat
     4561 gcgctgacat cccgaagcga gatctcgccc tcggcattga tgtaaacgcc tgtgatcgtc
     4621 tgcaaggcat cgccgacatt ctcgcaattg ttcttgacaa aatcttcctg cgttaaaaca
     4681 acctccgagg ccgatttttc tttctctttg ctatcctgag cctgggcata accagtgact
     4741 gccgaaatga tcaggatccc caaccagaga caaacggatt tgcgcatcat agagctactc
     4801 cttgccggtt atggtttgat agagcttccc ggtataccag tcgaaatcga tgtggttggt
     4861 tgcgctcaac agctgcaccc agtcgccggg aaaggcgtca atgcccctga tggcgccgca
     4921 gatggcgccg gtgatggcgc cgatggtatc acagtctccg cccagattgg cggcagcgat
     4981 aaccccctgc acgggatctc cgtcgacgag cctggtcagg gccaggcagg tcggaacggt
     5041 ctccgagatt tgcaccccgg cgccgatgac ccggtagagt ttttccagcg cctcagcccg
     5101 atctttcgtc cgtttgacaa tgtccagcgc cagattggcg cggtcaagaa tggaggcgct
     5161 gtaccagatg ttgcccagct gcatggccgg ttcaatgatc ccgatcgcgc tttggatcag
     5221 ggagtcgacg tcctgctcgc ctttcaaggc ctggccgatg gccatggcaa tggcggaggc
     5281 gccggcgatg gcgatattgg tgttgtgggt cgggatacag gctcggctga ccagatccat
     5341 ggttttatcc cggtctccga aacccaggat gccgaccgga gcgatgcgca tcaccgcgcc
     5401 attggtgtcg ccagcccggc ccgcttcatg gatcgatttt cccgatttta tggcatacaa
     5461 ggcccgcagc gagctggggc cgacggccat cgattcgaaa gcccggatct gctcccccca
     5521 ctggatcaga cgctgcgcaa tatcttccgg atcaaccccc tgccgggcga tgatcgagtc
     5581 agcgaacaac agggtttgct gggtatcatc tgtcaccgca ccggcggcca tcccgtcatg
     5641 aatgatatgg ccgggaggcg caggcaggaa ttggtcaata tatccgaaga ttttttttat
     5701 cgtctccggg ctcatcatcg agg
//
LOCUS       DAOPWU010000220         5673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14194_length_5673_cov_4.744571, whole genome shotgun sequence.
ACCESSION   DAOPWU010000220 DAOPWU010000000
VERSION     DAOPWU010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14194_length_5673_cov_4.744571"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKM23_10750"
     CDS             complement(<1..547)
                     /locus_tag="PKM23_10750"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY91991.1"
                     /translation="MKRSILNLLLPLGTALMVLSGSALAQGVSYKGTSAANFLKVAMG
                     AAYVAGGESDLTLAKDASSLWYNPGAISRIPKSSAVFSHSSWLVETSFINAAIALPLG
                     GFTVGMDLTYFGSGDIQETTLLKQDGTGRVVSASDLAIGAAVARNLTDRFSVGGKFKY
                     IREQLAQVSAGAFAFDIGSVFE"
     gene            complement(564..3110)
                     /locus_tag="PKM23_10755"
     CDS             complement(564..3110)
                     /locus_tag="PKM23_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY91992.1"
                     /translation="MKPRLLLFLCILPGFLLAQTTGKISGKIIDEKKEPVIGANIMID
                     GTAMGAAASVEGEYFIINIPPGTYNLKASAVGYGPKRVDKVKVEAGLTTRVNFTLQST
                     MIEMPEVVIQYTRPAVQKDLTSKMQGLDLSDIELMPTQMSIRDLFTKQAGITRDISTT
                     PVNSQPVFGQFATIPNDGLHFRGGRTNETLYLFDGITVNDGLWGGYNLDVLGQYTLQS
                     LQTLTGTFGPQYGEAMSGVILMQPVDNIATRWKAQAALLTDNLGEKSGSHNEYNVEAA
                     VSGPVPGVKNLTLASSARAYSSDGYINGYLYPNWVDSRGADKSGSPEVVPMTYRDTRM
                     AFGKAIWQVSKPLKLRLGYYAAETKQGVYDHFFKYNPYGTPRVTLNDYLGYFKLTHVF
                     SPSTFADFAISKYRRGYSSRVFSDLSLYQVRSEILTAEFSVAGENWVYFDSYYDKIEA
                     QGSFNSQVSQQHYLTSGLSLSTIKTSLQRRNPNGGDAYEDYFFKPKKLYAYVNDKMEF
                     DDIGMVINLGLRYDYIDPNRDFVEDIKSPDGKIGKVKPRQYISPRFGISYPISDAAAF
                     RFGYGHYYQYPDFYKAFQGSNREYSMYPAPNVNSVQGAIAKGDIQEEKTINYEFGVQM
                     QLAPKLSADITGFYRKTSNLIGIVIAEGYLTSGDIIKAQKYPIFDNINFATVKGVEFS
                     MTKRMSDNFTGFLNYTYSKALVSSSLVFSQAQDLARTFPADWDQSHVLSFGVTMEFPA
                     HWGYSLMGGMSSGFPYTFNVLMPNAERSPTQSYLDAMLFKEFTYSKITARLYGQVNNL
                     LNHKNVWWVYADSGQPGVDTSEITSDDYTNNPAMWGPGRRYQIGLNFNLD"
     gene            complement(3224..5515)
                     /locus_tag="PKM23_10760"
     CDS             complement(3224..5515)
                     /locus_tag="PKM23_10760"
                     /inference="COORDINATES: protein motif:HMM:NF027642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4957 domain-containing protein"
                     /protein_id="HNY91993.1"
                     /translation="MKKSALLLLILLASLASLLPAQTIVQVPAGTDGLAAAVAAATAG
                     DIIELTTNGGLYTETAKLVIDKKLTIRAASGLTQKPMLVSGAADAMIEFSTDFTLDGV
                     ILDGSLGADSTAVGITNAPNTTGYNLKVLKTDFLNFSDKAQTTGFGIFGVPSSVVDSV
                     LVQNCYFAHIMDMGISFNDPLTATGSVNLFKVDNCTFWDMNSEAIYIDAFDSKMDTKD
                     PEVIINQVTVYDCGSYNLIAHYIDYSVLTNSLVVLPELNTSYAPSKVYGTHSRVENFL
                     YFNTRDIDLSYGATDFQLINVLPQENPGLIDPENGNFSYPVTSPAALLAEDGTTIYLG
                     DERWWPKNIAEIVYLAAGENVLSTALAAAVDGQTLILTSNGGLYHESAKLIVDKKLTI
                     KAADELVQKPVISSDATDAMIEVSASLTLKGVVLDGALGGVPTTKGIVTKPQTSGYNL
                     AVLESDLLNFGNATGSTGFGIFGDPTAVVDSFLVQDCLFAHIVKMGISFNDPVIPTGS
                     VNLFKVENTTFWDINAEAIYVDGSDSDVATKDPEFFVNKVTAYDCGSYNIMAHYIDYS
                     VISNSIVVYPEPTTAYAPTRVYGTHSIVENLLYFNTRDIDLGSSATDFQLVNIAIQMD
                     PHFADPQNGDFHYALDSPAILFDDDGAPIYLGDDRWWPVTTGVNCAAPNSLPVEFSLA
                     QNTPNPFNPATTIHFSLPAAQRITLRVYNMLGEEVATLIDGALPAGLHKAQWNAAGMP
                     SGLYLYRLATENHAAVKRMMLLK"
BASE COUNT         1150 a         1414 c         1902 g         1207 t
ORIGIN      
        1 tctcgaagac gctgccgatg tcgaaggcga aagcgcctgc cgaaacctgg gcgagctgct
       61 cgcggatata tttgaatttt ccgcccaccg agaagcggtc ggtgaggttg cgcgccaccg
      121 cagcgccgat ggcgagatcg ctggcgctga cgacgcggcc ggtaccgtcc tgcttgagca
      181 gggtggtctc ctggatgtcg ccggaaccga aataggtgag gtccatgccg acggtgaagc
      241 cgccgagcgg cagggcgatc gccgcattga tgaaactggt ctccaccagc cagctggagt
      301 ggctaaagac cgcactcgat ttggggatgc ggctgatcgc cccgggattg taccaaaggc
      361 tggaggcgtc cttggccagg gtcaggtccg attctcctcc cgccacatag gcggcgccca
      421 tggccacttt gagaaagttc gccgccgacg tccccttgta gctgacgccc tgggcgagcg
      481 ccgatccgga caggaccatc agggccgtcc caagcggcaa aagcagattc aggatggatc
      541 ttttcatgca tcctctcctt ggatcaatca agattaaagt tcaggccaat ctggtagcgc
      601 cggcccgggc cccacatggc gggattattg gtatagtcat cagaggtgat ctcgctggta
      661 tccactcccg gctggccgga atcggcataa acccaccaga catttttatg gttgagcaga
      721 ttgttgacct gtccgtaaag cctggctgtg atcttggagt aggtgaactc cttgaagagc
      781 atggcatcga ggtagctctg ggtgggcgaa cgttctgcat tgggcatgag gacattaaag
      841 gtatagggga aaccgctgct catgccgccc atgagtgaat agccccagtg ggcggggaat
      901 tccatggtca cgccgaagga gaggacatgg gactgatccc agtccgccgg aaaggtgcgc
      961 gccagatcct gggcctgcga aaagaccagc gaactgctca ccagggcctt cgaataggta
     1021 tagttgagaa aaccggtgaa attatcgctc atacgcttgg tcatggagaa ctcgacaccc
     1081 ttgacagtgg cgaagttgat gttatcgaag atcggatatt tctgggcctt gataatgtcg
     1141 ccggaggtca gatagccctc ggcaatgacg atgccgatga ggttggaggt tttgcggtaa
     1201 aagccggtga tatcggccga gagcttgggg gccaactgca tctggacgcc gaactcatag
     1261 ttgatggttt tttcttcctg aatatccccc ttggcgatgg cgccctgaac cgaattgaca
     1321 ttgggggccg gatacatcga atactcgcga ttgctcccct ggaaggcctt gtagaaatcg
     1381 gggtactgat agtaatggcc gtagccgaag cggaacgccg cggcatcgga gatcgggtag
     1441 ctgatgccga aacgggggct gatgtactgg cgcggtttga ccttgccgat cttgccgtcc
     1501 ggggacttga tgtcctcgac aaaatcgcgg ttgggatcga tatagtcata gcgcaggccg
     1561 aggttgatga ccatgccgat gtcgtcgaat tccatcttgt cgttgacata ggcgtagagc
     1621 tttttcggct tgaagaaata gtcctcatag gcatcgccgc cattgggatt gcggcgctgc
     1681 agagaggttt tgatggtgga aagcgaaagt ccgctggtga ggtagtgctg ctgggagacc
     1741 tggctgttga aagagccctg cgcctcgatc ttgtcataat agctgtcgaa gtagacccag
     1801 ttttcgccgg cgactgaaaa ctcggcggtg aggatctccg accggacctg atagagggag
     1861 aggtccgaga agacccgcga gctgtagccg cggcggtact tggagatagc aaagtcggca
     1921 aaagtgctgg gcgaaaaaac atgggtcagc ttgaaatagc cgagatagtc gttgagggtg
     1981 acgcgcgggg tgccgtaggg attgtacttg aaaaagtggt catatacccc ctgtttggtc
     2041 tccgcggcat aatagccgag gcggagcttg aggggcttgc tcacctgcca gatcgccttg
     2101 ccgaaggcca tgcgggtgtc gcggtaggtc atgggcacca cttcagggga gccgctcttg
     2161 tcggcgccgc gcgaatcgac ccagttcggg tagagataac cgtttatata gccgtccgag
     2221 gagtaggcgc gtgccgagga ggccagggtg agattcttca caccaggcac ggggccgctg
     2281 accgcagcct cgacgttgta ctcattgtgg ctgcccgatt tttcacccag attatcggtc
     2341 aggagcgccg cctgggcctt ccagcgggtg gcgatgttgt cgacaggctg catgaggatg
     2401 acaccggaca tggcctcgcc atattggggg ccgaaggtgc cggtgagggt ctgcagggac
     2461 tggagggtgt actggccgag gacgtcgagg ttgtagccgc cccagaggcc gtcgttgacg
     2521 gtgatgccgt cgaagagata gagggtttca ttggtgcggc cgccgcggaa atggaggccg
     2581 tcgttgggga tggtcgcgaa ctggccgaag accggctggg agttgaccgg ggtggtgctg
     2641 atatcccggg tgatacccgc ctgtttggtg aagaggtcgc ggatggacat ctgggtaggc
     2701 atcagctcga tgtcgctgag gtccagaccc tgcatcttgg aggtcaggtc tttctggacg
     2761 gccggccggg tgtactggat caccacctcg ggcatctcga tcatggtgga ctgcagggtg
     2821 aaattgaccc tggtggtcag ccccgcctcg accttgacct tgtcgaccct tttcgggcca
     2881 tagccgacag cgctggcctt gaggttgtag gtgccgggcg gtatattgat gatgaaatac
     2941 tcgccctcga cgctggcggc tgcgcccatg gcagtaccgt cgatcatgat gttggcgccg
     3001 atgaccggtt cctttttttc atcgatgatc ttgccggaga tcttgcccgt ggtctgggcg
     3061 aggaggaatc ccggcaaaat gcagaggaat agaagcagtc tgggtttcac aggagcgcac
     3121 tcctttttcg ctaggtgtta caatcagccg tgaaccggaa gaagccgccg ggctggaagc
     3181 ggcgtataaa agcctgcacc gcttccagtc cgaacggatt gattcatttg agaagcatca
     3241 tgcgtttgac ggcggcatgg ttctccgtcg ccagccggta gagatagagg ccgctgggca
     3301 tgccggccgc attccactgc gccttgtgga ggccggcggg cagtgcaccg tcgatcaggg
     3361 tcgccacctc ctcgcccagc atgttgtaca ccctgagggt gatccgctgc gcggcgggga
     3421 ggctgaagtg gatggtcgtc gccggattaa agggattggg cgtattctga gccagggaga
     3481 actccaccgg cagactattc ggcgcggcgc agttcacgcc ggtcgtcacc ggccaccagc
     3541 ggtcatcgcc cagatagatc ggcgcgccat cgtcgtcgaa gaggatggcg ggcgaatcga
     3601 gggcataatg aaaatcgccg ttttgcggat cggcgaaatg gggatccatc tggatggcga
     3661 tgttgacaag ctggaaatcg gtcgcactgc tccccaggtc gatatcccgg gtgttgaaat
     3721 agagcaggtt ttcgacgatg gagtgggtgc cgtagaccct cgtcggggcg taggcggttg
     3781 tgggctcggg atagacgacg atggagttgg aaatgaccga ataatcgatg taatgggcca
     3841 tgatattgta gctgccgcag tcataggcgg tgactttatt gacgaagaat tccgggtcct
     3901 tggtcgccac gtcagagtcg gagccatcga cgtagatagc ctcagcgttg atatcccaga
     3961 aagtggtgtt ctccaccttg aagagattga ccgagccggt cgggattacg ggatcattga
     4021 agctgatgcc catcttgacg atatgggcga agaggcagtc ctggaccagg aagctgtcca
     4081 caaccgcagt gggatcgcca aaaatgccga agccggtcga tccggtggca ttgccaaaat
     4141 tgagcagatc gctctcaagg acggcaaggt tgtagccgga ggtctggggc ttggtaacga
     4201 tacctttggt ggtgggcaca ccgccgaggg cgccgtcgag gacaacgcct ttcagggtca
     4261 ggctggcgga gacctcgatc atggcatcgg tggcatcgga gctgatgacc ggtttttgta
     4321 ccagctcgtc tgcggccttg atggtgagct ttttatcgac gatcagcttg gccgattcat
     4381 ggtagagacc gccgttgctg gtgaggatca gggtctggcc atcgacggcc gccgccaggg
     4441 ctgtggagag gacattttcg cccgctgcga ggtagacgat ctccgcgatg ttcttcggcc
     4501 accagcgttc atcgccgagg tagatggtcg tgccatcttc ggcaagcagc gcggccggcg
     4561 aggtcaccgg gtaggaaaag ttgccgtttt caggatcgat gagtccggga ttttcctggg
     4621 gcaggacatt gatgagctgg aaatcggtgg cgccgtagct gaggtcgatg tcgcgggtgt
     4681 tgaagtagag gaaattctcg accctggagt gggtgccata aaccttggac ggggcatagc
     4741 tggtgttgag ttcggggagg acgaccaggg agttggtgag cacggagtaa tcgatgtaat
     4801 gggcgatgag gttgtagctg ccgcagtcgt agaccgtgac ctggttgatg atgacctcag
     4861 gatccttggt gtccattttg gaatcgaagg catcgatgta aatggcctcg gagttcatgt
     4921 cccagaaggt gcagttgtcg accttgaaga gattgaccga gccggtggcg gtgagtgggt
     4981 cattgaaact gatgcccata tccatgatgt gggcgaaata gcagttttgg acgaggacgg
     5041 aatcgacgac cgagctgggg acgccgaaga tgccgaagcc ggtggtttgg gccttgtcgg
     5101 agaagttgag gaaatcggtt ttgaggacct tgagattgta gccggtggtg ttcggggcat
     5161 tggtgatgcc gacggcggtg gagtcggcgc cgagcgagcc atcgaggatg acgccgtcga
     5221 gggtgaaatc agtcgagaac tcgatcatgg cgtcagcggc gccggaaacc agcatgggtt
     5281 tttgggtcag gccgctggcc gccctgatag tgagcttttt gtcgataacc agcttggcgg
     5341 tttcggtgta gaggccgcca ttggtcgtca gttcgatgat atcgccggct gtcgcggcgg
     5401 cgacagcggc agcgaggccg tccgtgcccg caggcacctg gacgatcgtc tgggcaggca
     5461 gaagggaagc cagcgaagcc agcagaatca gcagtagaag tgcacttttt ttcacgtaac
     5521 tcctcacttt ttaaggatta ttaaccgggc agccggcagc tgccctgact ttgcacgggt
     5581 gggcgcgcga ccggatggtc cgcgcgcccg ctgccttgtt atttcaggaa aaccattttt
     5641 ttaacctggt caaacgaacc cgactgcagg cgg
//
LOCUS       DAOPWU010000221         5661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14241_length_5661_cov_3.901534, whole genome shotgun sequence.
ACCESSION   DAOPWU010000221 DAOPWU010000000
VERSION     DAOPWU010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14241_length_5661_cov_3.901534"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="PKM23_10765"
     CDS             complement(<1..158)
                     /locus_tag="PKM23_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNY91994.1"
                     /translation="MLDQAFTGAEAYRVHLTFPFIAISGSNGKTTVKAMLAHILRKVG
                     RVYEFSGDS"
     gene            complement(293..1771)
                     /locus_tag="PKM23_10770"
     CDS             complement(293..1771)
                     /locus_tag="PKM23_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY91995.1"
                     /translation="MKIERANILVVDDEFGMREGIRRLFEAQGHRVETAETGRNGIDK
                     GTAEEFDIYFLDLKIGDIDGVEVLGAIKAHFPEAICVICTAYASIETAVETTRLGAYN
                     YIAKPFAPEEIQLVFDRAIERRWYILEARQLRAEQERRLLEITQEKSRIRTIINSIAD
                     GIVVLNQDEEVVLFNPQFPRLLELDKELVIGRSIREVLPEPYQELIADIFRQKDSLQA
                     LQQEFIIHPPAKLVVMANTTTIRDDQNRLIGYVSVLRDISELKQLELLKSQFVNMAAH
                     ELKAPLTAIQGFLEMVVDKTLGDEPETYDNYLRRSLERSKTLITLINDLLNISRIQAG
                     KIRREIERLDLSQQAHSAADFFANEIEKHELTLETEFADGLVIDADREEIQRLLTNLI
                     SNAIKYNRPQGSVTLRTSREKGSARLDVSDTGIGMKPEEKERLFEEFFRAKNPHTRSI
                     TGTGLGLTLVKKIVDSYAGRIEAESEYEKGTTFTLYLPLAER"
     gene            complement(1856..2293)
                     /locus_tag="PKM23_10775"
     CDS             complement(1856..2293)
                     /locus_tag="PKM23_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY91996.1"
                     /translation="MKKEDTSAMKKKIMMVDDDYDLLQIYVPVLEKAGFEVATANTAA
                     EGLELFKTFLPDLVMVDLAMEHFDSGFVLCHRIKTLPEGAQTPVVIMTAASHETGIRF
                     STQTEEERKWIQADEYLDKPIPPRELLAFIKERFFETPESQQH"
     gene            complement(2296..2685)
                     /locus_tag="PKM23_10780"
     CDS             complement(2296..2685)
                     /locus_tag="PKM23_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY91997.1"
                     /translation="MSKKILLIDDDIDIVELNRALLTMAGYEVVTAFSGAEGLEKVVA
                     ERPDLVILDVMMSTVGEGFEVARQIRKNQEIRHTPILMLTGVNREHPFNLRIGPDVEW
                     NPVDSFIEKPVQKEVLLAKVNAMLQDN"
     gene            complement(2682..4733)
                     /locus_tag="PKM23_10785"
     CDS             complement(2682..4733)
                     /locus_tag="PKM23_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNY91998.1"
                     /translation="MSVIWTIEERCKSCYSCIRECPAKAIMVVKGQAKVVEELCLGCG
                     HCVRVCTQGAKAVKSGVEAVERFLKGSHPVVAMIAPSFPAGLPGVEGEQLSGALRKAG
                     FSHVVEVAFGADLINREYYRLVNSKQTQDGREIALPLITSSCPAIYELVERTAPDLVP
                     NLAPIVSPMIAMGRVVRRCYGEEVRTVFIGPCTAKKVEMVDPEVSDAVDEVLTFAELK
                     ELWKHLDIDPAQAEPAGFDPPHAYLGRLYPVSGGLLRSAGLPFDLMNNEVVVTEGKQR
                     VLNLLDSLKNHEVKASLVDILFCEGCISGPFTDTSLNFFTRKQKIVSYTDSLRMHTNY
                     AEWKKAILQYQDVVIRRSFHPMVKEKPEPTEDEIRLILKEIKKAISSDELNCGACGYE
                     TCREYAKAVFQGFAEEEMCLPYVIEELQHTQKELQESLRDLAEAQEQLIQHEKLASIG
                     QLAAGVAHEVNNPLGSIMLYAHLLLQQLKEDDQKSKDLHFIMDEAKRCQKIVAGLLNF
                     ARQGKLNLRKVALQEILDKLVSVAFNQPLFRMTQVVTRFEPDLPPIEVDYDQIYQVFL
                     NLAVNAAEAMPEGGVLTIQAALTPDRQSMRVAFCDTGIGIPAENMNKLFTPFFTTKQI
                     GKGTGLGLAIAYGIIKMHRGSITVRSEPHQGSTFTVELPIQNTVHTNAFDFKAVQESM
                     K"
     gene            complement(4758..>5661)
                     /locus_tag="PKM23_10790"
     CDS             complement(4758..>5661)
                     /locus_tag="PKM23_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNY91999.1"
                     /translation="GADLTIIEEASELLHRVKSGGKLPLLTSCSPGWVKYIEHFYPDL
                     LGHVSSCKSPHEMEGALIKSYYAEKTGIPAKDIYVISIMPCIAKKFEAQRPELKEVFA
                     DVDAVLTTRELARMIKNAGIDFTKLNDEHFDDPMGESTGAAAIFGAAGGVMEAALRTA
                     HFFLTGKEMDNVEFTPVRGLQGVKEGEVTIAGLTIKIAAVSGLKNISAILDKIRSGEN
                     TYQFIEVMACPNGCINGGGQPINSDPDRVRKRMEAIYAIDRTLPKRASHQNEAVMRLY
                     KEYLGEPNGHKAHELLHTHYVERS"
BASE COUNT         1085 a         1514 c         1771 g         1291 t
ORIGIN      
        1 gagtcaccgg agaactcgta tacccgcccg accttgcgca ggatatgagc gagcatggcc
       61 ttgaccgtcg tcttgccatt gctgcctgaa atagcgatga atggaaaagt taaatgtaca
      121 cgatatgctt cagcgccggt gaaggcctga tcaagcataa tggtcatcct gcaaattcac
      181 cgtttgggtt agccagtgag gagcacggaa tcaggctccg agaggaccgc acaggtgcaa
      241 aaataagtaa attttagcaa agaagaaaga aaataaatcg gcagacctcc ttttatcgct
      301 cagcgagggg gagataaagg gtgaaggtgg tgcccttttc gtattcggat tccgcctcga
      361 tgcgtcctgc gtagctgtcg acgatctttt taaccagggt gagccccagt ccggtgccgg
      421 tgatgctgcg ggtatggggg ttcttggcgc ggaaaaattc ctcgaagagg cgctccttct
      481 cctccggctt catgccgatg ccggtgtcgc tgacgtccag ccgggcacta cccttttcgc
      541 ggctggtgcg cagggtgacg gagccctggg ggcggttgta cttgatggcg ttggagatca
      601 ggttggtgag gagtcgctgg atctcctcgc ggtcggcgtc gatgaccagc ccatcggcga
      661 attcggtctc aagggtcagt tcgtgttttt caatttcatt ggcgaagaaa tcggcggcgg
      721 agtgggcctg ctgggagaga tccaggcgtt cgatctcgcg gcggatcttg ccggcctgga
      781 tgcgtgagat gttgagcagg tcgttgatga gggtgatcag agtcttgctg cgctccagag
      841 agcggcggag atagttgtca taggtttcgg gttcatcacc gagggttttg tcgacgacca
      901 tctcaagaaa gccctggatg gcggtcaagg gtgccttgag ctcatgggcg gccatattga
      961 cgaactggga tttgagcagt tcgagttgtt tgagttcaga gatgtcgcga aggacggaga
     1021 cgtagccgat caagcggttc tgatcgtcgc ggatggtggt ggtgttggcc atgacgacca
     1081 gttttgccgg gggatgaatg atgaattcct gttgaagggc ctgcaggctg tccttttgcc
     1141 gaaagatgtc ggcgatcagc tcctgatagg gctccggcag cacctcgcgg atggagcggc
     1201 cgatgaccag ttccttatcg agttcgagca gccgggggaa ctgtggattg aagaggacca
     1261 cctcctcatc ctgattgagc acgacgatgc catcggcgat cgaattgatg atggtgcgga
     1321 tacgggactt ttcctgcgta atttcaagca ggcgcctctc ctgttcggcc ctgagctggc
     1381 gcgcttcgag gatataccag cgcctctcga tagcgcggtc gaagacgagc tgtatctctt
     1441 ccggggcgaa gggcttggcg atgtagttat aagcgcccag cctggtggtc tcgacggctg
     1501 tttcgatgga ggcatacgct gtgcagatga cgcagatcgc ttcagggaaa tgggccttga
     1561 tggcgccgag gacctccacc ccgtcgatat cgcctatctt gaggtcgagg aagtagatat
     1621 cgaactcttc ggcggtccct ttgtcgatcc cgttgcgtcc cgtttcagcg gtctcgaccc
     1681 tgtggccctg ggcctcaaag aggcgccgaa tgccctccct catgccgaat tcgtcgtcaa
     1741 caacgaggat atttgcgcgt tcgatcttca tgcctgccca ctgagtgaag ggttatgcgc
     1801 gaaggcagca cctgcgccgt ttttttgcat tggcgggggt gctgcctgtg tgcgttcagt
     1861 gctgctggga ctcgggggtc tcgaagaagc gctccttgat gaaggcgagc agctcgcgcg
     1921 gaggaattgg cttgtccagg tattcgtcgg cctggatcca tttgcgctcc tcctcggtct
     1981 gggtggagaa gcggatgcca gtctcatggc tggccgcggt catgatcacc accggggtct
     2041 gagccccctc gggcagggtc ttgatgcggt ggcagaggac aaaacccgag tcaaaatgct
     2101 ccatggccag atcgaccatc accaggtcgg ggagaaaggt tttgaacagc tcaagtccct
     2161 cagcggcggt attggccgtc gccacctcaa aaccggcctt ttcgagcacc ggaacgtaga
     2221 tctgcagcag atcatagtcg tcatcgacca tcatgatttt tttcttcatg gcgctggtat
     2281 cttccttttt caatgttaat tatcctgcag catggcgttg accttggcga ggaggacctc
     2341 tttctggaca ggcttttcta taaagctgtc caccggattc cactcaacgt cgggtccgat
     2401 gcgcagattg aagggatgct cccggtttac gccggtgagc atcagaatgg gggtgtggcg
     2461 gatctcctgg tttttgcgaa tctgccgcgc cacttcaaag ccttccccga ccgtgctcat
     2521 catcacgtcc aaaatgacca gatccgggcg ctccgcgacc accttttcca gcccctcggc
     2581 gccgctgaag gcggtcacta cctcatagcc ggccatggtg agaagggcgc gatttaattc
     2641 gacgatatcg atgtcatcgt ctatcaacaa gatttttttg ctcatttcat gctctcctgc
     2701 acggctttga aatcgaaagc atttgtgtgg accgtattct gaatcggcag ctcaaccgta
     2761 aatgtggagc cttgatgggg ctcgctcctg acggtgatgc tgccgcggtg cattttgata
     2821 atgccatagg cgatggccag gccgaggccg gtccccttgc cgatctgctt ggtggtgaag
     2881 aagggggtga acagcttgtt catgttctcc gccgggatgc cgatgccggt gtcgcaaaag
     2941 gcgacgcgca tcgattgccg gtccggggtg agtgcggcct ggatggtcag aacaccgccc
     3001 tcaggcatgg cttcggcggc gttgaccgcc agattgagaa aaacctggta aatctggtcg
     3061 taatcgacct cgatgggcgg caaatccggt tcgaaccggg tcaccacctg ggtcatgcgg
     3121 aaaaggggct gattgaaagc cacggagacg agcttgtcga gaatctcctg cagggccacc
     3181 ttgcgcaaat tgagcttgcc ctgacgagcg aaattgagca gcccggcgac gatcttctgg
     3241 cagcgtttgg cctcatccat gatgaagtgc agatccttgc tcttctggtc atcctccttc
     3301 aactgctgga ggaggagatg ggcgtaaagc atgatcgaac cgaggggatt gttgacttcg
     3361 tgggccacac cagctgccag ctgcccgatc gaggccagtt tctcgtgctg aatgagctgc
     3421 tcctgggctt cggccagatc gcgcagggat tcctggagtt ctttctgggt atgctgaagc
     3481 tcctcgatga cgtagggcag gcacatctcc tcttccgcaa agccctggaa gaccgccttg
     3541 gcgtattcac ggcaggtttc atagccgcag gccccgcagt tgagttcatc ggaggagatg
     3601 gctttcttga tctccttgag gatcagcctg atctcgtctt ctgtgggttc cggtttctcc
     3661 ttgaccatgg gatggaagga gcggcggatg acgacatcct ggtactgcag gatcgccttc
     3721 ttccactctg catagttggt atgcatgcgc agggaatcgg tataagagac gatcttttgc
     3781 ttgcgggtga aaaagttcag cgaggtgtcc gtgaagggcc cgctgatgca gccctcgcaa
     3841 aagaggatgt caaccaaact cgccttgacc tcatgatttt tgaggctgtc aagcagattg
     3901 aggacgcgct gtttgccctc ggtgaccacc acctcgttgt tcatcaggtc gaaggggagg
     3961 cctgcgctgc gcaagagccc tccggaaacc ggatagaggc gtccgagata agcgtggggc
     4021 ggatcgaatc cggccggttc agcctgggcg ggatcgatat cgaggtgttt ccacagttcc
     4081 ttcaactcag caaaggtgag cacctcatcc acggcatcgg agacctcagg atcgaccatc
     4141 tctacctttt tggctgtgca ggggccgatg aagaccgtcc tgacctcctc gccgtagcag
     4201 cggcgaacca cccttcccat ggcgatcatg ggactgacga tcggggcgag gttgggcacc
     4261 aggtccgggg ccgtgcgctc gaccagttca taaatggccg ggcaggagct ggtaatcagg
     4321 gggagggcga tctcacggcc gtcctgggtt tgtttggagt tgaccaggcg gtagtattcc
     4381 cgattgatca ggtctgcgcc gaaggcgact tcgaccacat ggctgaatcc ggccttgcgc
     4441 agggcgcctg agagctgctc cccctccact cccggcaagc cggccggaaa agagggggcg
     4501 atcatggcga cgacaggatg gctgcccttg agaaagcgct ctaccgcctc cacgccgctc
     4561 ttgaccgcct tggccccctg ggtgcagacg cgcacgcagt ggccgcagcc caggcaaagt
     4621 tcttcgacga ccttggcctg gcccttgacg accatgatgg ccttggcggg gcattcacgg
     4681 atgcaggaat agcagctttt acagcgttct tctatggtcc atatgacgga cataaagcca
     4741 tccttgccgg tttaaggcta ggaacgttcc acgtaatggg tgtggaggag ctcgtgggcc
     4801 ttgtggccgt tgggttcgcc gaggtactcc ttgtagaggc gcataaccgc ctcattctgg
     4861 tgcgaggcac gtttgggcag ggtgcggtcg atggcataga tggcctccat acgcttgcgc
     4921 acccggtcag gatcgctgtt gatgggctgg ccgccgccgt tgatgcagcc gttcgggcag
     4981 gccatcacct cgatgaactg gtaggtgttc tcaccgctgc ggatcttgtc gagaatggcg
     5041 ctgatgttct tcagcccgct gaccgcggcg attttaatgg tgagcccggc gatggtgacc
     5101 tcgccctcct tcaccccctg caggccgcgc accggggtga attcgacgtt gtccatctcc
     5161 ttgccggtga ggaaaaagtg ggcggtgcgc agtgcggctt ccatgactcc gccggcagcg
     5221 ccgaaaatgg ccgctgcacc ggtcgattcg cccatcggat catcaaaatg ttcatcattg
     5281 agcttggtga agtcgatacc ggcattcttg atcatgcgcg ccaattcccg cgtggtgagc
     5341 acagcgtcga catcggcaaa gacctccttc aattcggggc gctgggcctc aaattttttg
     5401 gcgatgcagg gcatgatcga gatcacatag atgtccttgg ccgggatgcc ggtcttctcc
     5461 gcgtaatagg atttgatcag ggccccttcc atctcgtgcg gggacttgca ggaggagacg
     5521 tggccgagga gatcgggata aaagtgctcg atatacttga cccagcccgg gctgcaggag
     5581 gttagcagag gcagtttgcc gccgctcttg acgcggtgga ggagttcgga ggcctcctcg
     5641 atgatggtga ggtcggcgcc g
//
LOCUS       DAOPWU010000222         5628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14344_length_5628_cov_3.594114, whole genome shotgun sequence.
ACCESSION   DAOPWU010000222 DAOPWU010000000
VERSION     DAOPWU010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14344_length_5628_cov_3.594114"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(379..1023)
                     /locus_tag="PKM23_10795"
     CDS             complement(379..1023)
                     /locus_tag="PKM23_10795"
                     /inference="COORDINATES: protein motif:HMM:NF025429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HNY92000.1"
                     /translation="MEVTSLNDIFAPFAGNGLIEWFEDLTELWHETGPIPPDDHLSPK
                     GMTQSLHYHNYWLWHQEDEARRTDLPDKDIAAAKRAIDSHNQHRNDGIEKIDLWIDNV
                     LNAAGINPGPEIEVNSETPGSMVDRLSILSLKIFHMDEQALREELAPDLQELCVIRSN
                     VLREQRQDLAHALDKLLLDLRKVNKRHKVYRQFKMYNDPRFNPAIYLNKREESR"
     gene            1346..2380
                     /locus_tag="PKM23_10800"
     CDS             1346..2380
                     /locus_tag="PKM23_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNY92001.1"
                     /translation="MPLPLETILLERLFRKCYGETPDRINPLKGDGSERRIYRMQNVY
                     RSVIGVAGDNRPENEAFYHFSSHFARYNLPVPAIYAAELENNIYIEQDLGFFTLFEWM
                     QPLREKEGFGGSVMAMYKKVLSWLPRFQITAGRTLDYSYCYQHALFGRDSMMWDLRYF
                     QHRFLAPFYSAPLDQTALGGDFTRLVSHLLEVPADSFLYRDFQSRNVMILDNEPHFID
                     YQSGRRGALQYDVASLLFDAKANIPEPEREELLDYYLQALQEVEMVDEEKFRSYFYSF
                     VLMRVMQALGAYGYLAAVKGKKSFLKSVPFALANLEILLQKAAVLEQLPTLRGVFSRL
                     IQDPALREIK"
     gene            2395..2799
                     /locus_tag="PKM23_10805"
     CDS             2395..2799
                     /locus_tag="PKM23_10805"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNY92002.1"
                     /translation="MNVKKLTIVIHSFGYHVSGIPHDSSGHGGGYVFDCRGLPNPGRD
                     PRFLRLTGEDEGVQEYLSQQPEVVLFLDHAEALIRISADNYQARGFERLMVSFGCTGG
                     QHRSVFCAVQIAGRLRAAGYAVELKHQDMPHF"
     gene            2799..3530
                     /locus_tag="PKM23_10810"
     CDS             2799..3530
                     /locus_tag="PKM23_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNY92003.1"
                     /translation="MKAMILAAGYGTRLHPLTLTLPKALVPLAGKPLLGIILERLAAA
                     GFDQIAVNAHHFSIQIRAWLELHPLPGVQIHLSEEEEILGTGGGIKRMISLLGDDKPI
                     LVHNVDVLSALPLRSFYLSHTRGRAVATLAVQARSTKRYLVFDSRGDLCGRAGVEDGE
                     AELVRAPQGRLRYLAFNGIQVIEPALFVGQAEKEFSSVNLYLEAAREQSVRGWRMDAW
                     YWRDLGKPVDLEAAEREIAAGLIRI"
     gene            complement(3537..4553)
                     /gene="add"
                     /locus_tag="PKM23_10815"
     CDS             complement(3537..4553)
                     /gene="add"
                     /locus_tag="PKM23_10815"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNY92004.1"
                     /translation="MGEELDSDFLQRMPKVELHLHLEGAIQPATAIDLMRRNGADYVP
                     ESSLDLKRLYRFEDLSQFVTALRSVSNHIQRLEDLQRIARELLDNLITQNVRYIEFDC
                     AVQKYLDLGFTLDEILAALQECAEEAADRIEARLVINLQRSHGPQKTAELVERTAALD
                     HPLVAGIGLSGDESRYPQRDYRRAFDLAREAGLHRTVHAGEALGPDSVRDALDLLHAE
                     RIDHGTRAIEDPVMVDRLREEQIPLTQCLSSNLRLNIIPDLHSHPFPLFLRQGLVVAL
                     HTDDPQVFGITLTGEYQLAHSGFSLTRDEIRRIVFNGVQGAFLPENRREELHRRITEA
                     WAAL"
     gene            complement(4636..>5628)
                     /locus_tag="PKM23_10820"
     CDS             complement(4636..>5628)
                     /locus_tag="PKM23_10820"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNY92005.1"
                     /translation="AGAARHGASTQVCDQIWEMILSFSEYSFCKAHSASFALVSFQSA
                     WLRVHYPAEFIAAVISNQGGYYSTFAYISEARRMGLEILPPDINLSRSAYSGRDRQVR
                     VGLMQLKELSREGREALLAERLRGGSFSSLANLLARLNITPSDCAILIKAGCFDAVEK
                     GRSRPQLLWQMRHHFSRRTDPALGTLSLFDLAPPETERLPQPPDYDLRDLLRMEEEIL
                     GFLLSHHPLELYREKIARIRHIAGRDLDQHVGEEVTTIGWLITAKMATTRKRELMEFL
                     SFEDTTAIYETTFFPQAYARFAHMMTRTRPYVLRGVVDEQFGAVSLIVDNVTFL"
BASE COUNT         1131 a         1529 c         1744 g         1224 t
ORIGIN      
        1 ggcatcaaca ttgcttaaag tgccgatctt gttcatatat caccagcctc cttcgaggca
       61 cgtcagcgag gacgcgcctg atatcatgta tgtcgaatgg tgcgtaatta tgctgttaaa
      121 aataaaatag atataatata cataaaaata ttaaaatatc aaataaaatc gattgttttt
      181 ccacgaaact atgcagaaat cgcagagcgg gcgaattgtt tcgtgctggt ctgggccggt
      241 gagaagcctt gtagtgaaac gacgcccgga ggcaagagtt ccccgttttg acaaaatggc
      301 tgtgactggc agacgagcgg cactatcggg atcgagtaca ggtgccgtga ggcggagggg
      361 gagcgaaagg agagcgccct agcggctctc ctcgcgcttg ttgagataga tcgccggatt
      421 gaagcgggga tcgttgtaca tcttgaactg gcggtagacc ttgtggcgct tgttgacctt
      481 gcgcaggtcg aggagcagct tgtccagggc gtgggcgaga tcctggcgtt gttcgcgcag
      541 gacgttgctc cggatgacgc acagctcctg gaggtcaggg gcgagttctt cgcgcagggc
      601 ctgctcgtcc atatgaaaga tcttcagcga gagaatggaa agccggtcga ccatgctgcc
      661 gggggtttcg gaattgactt cgatttcagg gccggggttg atgccggcgg cattgaggac
      721 gttgtcgatc cagaggtcaa tcttttcgat gccgtcattg cgatgctggt tatgggagtc
      781 gatggcgcgc ttggcggcgg cgatatcctt gtccggcagg tcagtgcggc gcgcctcatc
      841 ctcctggtgc cagagccagt agttgtgata gtggagcgat tgggtcatgc ccttgggtga
      901 gagatggtca tccgggggaa tgggaccggt ttcatgccaa agttcggtga gatcctcgaa
      961 ccattcgatc agtccgttgc ccgcaaacgg cgcaaagatg tcattaaggg atgtcacttc
     1021 cacacctcgg attttggtaa attctgcgct gctgtgcaca cccgcgaggg cgacgcccac
     1081 agccacacac ctccggatag gttccggttg tgcttaatgt aacacagatc ggtggaaaac
     1141 gcaagcaaat ttcatggccg ccttgatttc ctccgctgct tcgtgcgccg aaacgggatt
     1201 gccgggtagc gcggcagcct ggcttcggaa tgagaccggc gggttctgcc cttcagcgtt
     1261 actccgcccc cttttcaaga attcaaattg tatttttgca taaatatgtt tatgttttgt
     1321 tcaattccag ctcatcgtta accggatgcc gctgcccttg gaaaccatcc ttctcgaaag
     1381 actcttcaga aaatgttacg gcgaaacgcc ggaccgcatc aatcccctca agggggatgg
     1441 ttcggagcgt cgcatctacc gcatgcagaa tgtttatcgt tccgtgatcg gggtcgcggg
     1501 cgataaccgg ccggagaatg aagcctttta ccacttcagc agccatttcg cccgctacaa
     1561 cctgccggtg ccagcgatct atgccgccga actggagaac aacatttata tcgagcagga
     1621 tctgggcttt ttcaccctct tcgaatggat gcagccgctg cgggagaagg agggtttcgg
     1681 agggtcggtg atggcgatgt ataaaaaggt cctctcctgg ctgccccggt tccagatcac
     1741 cgccgggcgc accctcgatt acagctattg ctatcagcat gccctcttcg gccgtgattc
     1801 aatgatgtgg gatctgcgct atttccagca ccggtttctc gctcccttct acagcgcccc
     1861 actggatcag acggcgctcg gcggggattt cacccggctg gtgagccatc ttctcgaggt
     1921 gccggcggac agcttccttt acagggattt tcagtcgcgt aatgtgatga tcctcgacaa
     1981 cgagccccat ttcatcgatt accagtccgg acgccggggc gccctgcagt atgatgtcgc
     2041 ctccctcctt ttcgacgcca aggccaacat ccccgagccc gagcgggaag agcttctcga
     2101 ttactatttg caggccttgc aggaggtcga aatggtggat gaggagaagt tcaggagcta
     2161 cttttacagt tttgtcctca tgcgggtcat gcaggccctg ggggcatacg ggtatctggc
     2221 agcggtaaag ggtaagaaga gctttctcaa atcggtgccc ttcgccctgg ccaatctgga
     2281 gattctgctg caaaaggcgg ccgtgctcga acagctgccg acgctgcgcg gagttttttc
     2341 ccggttgatc caggatccgg ccctgcgcga aatcaaataa ctccggagat gctgatgaat
     2401 gtcaagaaac tgaccatcgt aatccacagc tttggctatc atgtttccgg catcccccat
     2461 gattcctccg gccatggcgg ggggtatgtc tttgactgcc gcgggctgcc caatcccggc
     2521 cgtgatcccc gttttctcag gctgaccggt gaagatgaag gagtccagga atacctatct
     2581 caacagccgg aggtggtact ttttcttgac catgcggaag ccctgatcag aatcagcgcc
     2641 gacaactatc aggcgcgtgg attcgaacgg ctcatggtca gcttcggctg cactggtgga
     2701 cagcaccgct cggtgttttg tgcggtgcag atcgccggcc gtctgcgcgc cgccggatat
     2761 gcggttgaat tgaagcatca agacatgcct catttctgat gaaagcgatg attctggcgg
     2821 cgggttatgg cacccgcctc caccctctca ccctcacact ccccaaagcc ctggtgcccc
     2881 tggcgggaaa acccctgctc gggattatac tggagcgtct ggccgcggcc ggattcgatc
     2941 aaatcgccgt caacgctcac catttttcaa tacagatcag ggcctggctt gagcttcatc
     3001 ctcttccagg cgttcagatc cacctttcag aggaagaaga gatcctcggc accggcggcg
     3061 gcatcaagcg catgatctcc ctgctcgggg atgataagcc gatcctggtc cacaatgttg
     3121 atgttctctc cgccctgccc ctgcggtcat tctacctgtc ccatacgaga ggccgggccg
     3181 tggcaaccct ggcggtgcag gcgcgctcaa ccaaaaggta tctggttttt gacagccggg
     3241 gcgatctctg cggccgggcc ggggtcgagg acggcgaggc ggagttggtg cgggcgccgc
     3301 agggcaggct ccgctatctc gcctttaacg ggatccaggt gatcgagccc gccctctttg
     3361 ttgggcaggc ggagaaggaa ttttcgagtg tcaatttgta tctcgaggcg gcgagggagc
     3421 agagcgtgcg gggatggagg atggatgcct ggtactggcg tgatctcggc aagcccgtcg
     3481 atctcgaagc ggcggagaga gagatcgctg cggggttgat ccgtatttag gggggatcag
     3541 agcgctgccc acgcctccgt gatgcgccga tgcagctctt ccctgcggtt ttcaggcaga
     3601 aaagccccct gcacgccgtt gaaaacgatg cggcggattt catcccgggt caggctgaag
     3661 ccggagtgcg cgagttgata ttcaccggtc agggtgatgc cgaaaacctg gggatcatcg
     3721 gtgtggaggg cgacaaccag tccctgacgc aggaagaggg ggaaaggatg ggagtggaga
     3781 tcggggatga tgttgagcct gagattgctg ctcaggcact gggtgagagg gatctgctcc
     3841 tccctgaggc ggtcgaccat aacgggatcc tcgatggcgc gggtgccgtg gtcgatgcgt
     3901 tcggcgtgga gcagatcgag ggcgtcgcgg acgctatcgg ggccgagagc ctcacctgcg
     3961 tgcacggtgc gatggagtcc cgcctccctg gccagatcga aggcgcggcg gtaatcccgc
     4021 tggggatagc ggctttcatc gccggagagg ccgattccgg cgaccaaggg atgatcgaga
     4081 gcggcggtgc gctcgaccag ctcggcggtc ttttggggac catgtgaccg ctgcaggttg
     4141 atgaccaaac gcgcctcgat ccggtccgca gcctcctctg cacactcctg cagggcagcg
     4201 aggatctcgt cgagggtaaa acccaggtcg aggtacttct gtaccgcgca atcgaactcg
     4261 atgtagcgaa cgttttgggt gatgaggttg tccagcagct cgcgggcgat gcgctgcagg
     4321 tcctcaagcc gctggatgtg gttcgagacc gaacgcaggg cggtgacgaa ttgcgaaagg
     4381 tcttcgaacc ggtagagccg tttgaggtcg aggctgcttt cggggacata gtcagccccg
     4441 ttgcgccgca taagatcgat ggcagtcgcc ggttgtatcg ccccttcgag gtggaggtgc
     4501 agttccacct tcggcatccg ctgtaaaaaa tcgctgtcca attcttctcc catcgttccc
     4561 ctgttcagcc ctcccggcct ttttctctct ctccggttaa tatagcgtaa aaataatgaa
     4621 atagccagta taaagtcata aaaacgtgac attatccaca atcaggctga cggccccgaa
     4681 ttgctcatcc accacccccc tgagcacgta ggggcgggtg cgggtcatca tgtgggcaaa
     4741 gcgggcgtag gcctggggga aaaaggtggt ttcgtagata gcggttgtat cctcgaaact
     4801 gaggaattcc atcagctccc ttttgcgagt ggtcgccatc ttggcggtaa tcagccagcc
     4861 gatggtggtc acctcctcgc cgacgtgctg gtcgagatcc cggccagcga tgtggcggat
     4921 gcgggcgatc ttctcccgat agagttccag tggatggtgc gagagcagga atccgaggat
     4981 ctcctcttcc atgcgcagga gatcgcgaag atcataatca ggaggctgcg gcaggcgctc
     5041 tgtctcagga ggcgccagat cgaagagcga gagggtgccg agcgccggat ccgttcttct
     5101 gctgaaatga tggcgcatct gccagagcag ctgcgggcgg ctcctgccct tttcgacggc
     5161 gtcgaagcag cccgctttga tgaggatagc gcaatccgag ggtgtaatgt tgagccgggc
     5221 gagcaagttg gcgagactgc tgaaggatcc gccgcgaagg cgttcagcga gcagggcctc
     5281 ccttccctcg cggctcaact ccttgagctg catcagtccc acccgaacct ggcgatcccg
     5341 gccgctgtag gcagaacggc tgaggttgat atcgggcggc aggatttcga gtcccatacg
     5401 ccgggcttcc gagatatagg cgaaggtcga gtagtagccc ccctggttgc tgatgacagc
     5461 ggcaatgaat tccgccggat agtgcacgcg cagccaggcg gactggaagg agaccagggc
     5521 gaaggaggcg gaatgagcct tgcagaaact gtactcggaa aagctgagaa tcatctccca
     5581 gatctggtcg cacacctgcg tcgaggcacc atgtcgggcg gcgccggc
//
LOCUS       DAOPWU010000223         5614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14393_length_5614_cov_4.370930, whole genome shotgun sequence.
ACCESSION   DAOPWU010000223 DAOPWU010000000
VERSION     DAOPWU010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14393_length_5614_cov_4.370930"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PKM23_10825"
     CDS             <1..1056
                     /locus_tag="PKM23_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HNY92006.1"
                     /translation="ELVLKDSESSIQQGIRAMQELLDSHVDLVIGELAGDRSAAMAAL
                     ASERRIPMLVPVSSDNGIAALGTGVYQMTSDLETRGAALARYAFNTLGLRTFVSLAPA
                     DDYGQAMSDAFANTIDKLGGTIIAQQWYFPGTQDVSRQFGAIRESASQFITPDAGAMA
                     AEAVDSGQNEKAEYRYTRPTRRPAATPEDEEDIPQLRSIDGFFMPAYAEDISIIAPQF
                     SLAHIKAVPLGGDDWLHGSALKSQRRYLDGAVFCAGAFADETGMEYIQFKNRFRMTTG
                     TTAGPLAIAGYDLTHLITTAIQAGNRNAAGVIDWLSRTQKRSGIANGYSFAPGVRVNR
                     DVMILQYKEGNISRLLY"
     gene            1105..1428
                     /locus_tag="PKM23_10830"
     CDS             1105..1428
                     /locus_tag="PKM23_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92007.1"
                     /translation="MDDVLKKAMMAGLGIFSTTKEKIEEFVEEMIQKGEMEQGDKAKV
                     VQETMDKVEARATEAKDWIAKQVEETWEKMKPKAQAQLDELQEKLAGLSRELEEMKAK
                     MGPKE"
     gene            1476..2240
                     /locus_tag="PKM23_10835"
     CDS             1476..2240
                     /locus_tag="PKM23_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92008.1"
                     /translation="MKKLLVVVFCMLVPFTMAFAQKTDGVHLFQNFFNDAVIAGNPYG
                     EAGLGYATYDGGSTITGGVQGGLPVGKNLELNVGVHYLNWSYDNDDADSQGGLADPYV
                     GLRYQLTPGPTRFAVGGYATLPVGSEDIGQGHLNFGAFGAVRHKLEAFTLTGTVGINF
                     YEYTDYEFDMDTFEVEESTERDNYLTLGAGIIYPTSKNLAIVGEWVLKTEMDFMMLSG
                     GVDYNMGNVRYRGALGLGLDDGAPDFQILASYLLQF"
     gene            2432..3241
                     /gene="uppP"
                     /locus_tag="PKM23_10840"
     CDS             2432..3241
                     /gene="uppP"
                     /locus_tag="PKM23_10840"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HNY92009.1"
                     /translation="MTLLNSILLGIVQGLTEFLPVSSSGHLVLFSHFLGVEDAGITFE
                     VLLHFGTILAVLVAFRRDIGQLLTTFFGLFRSGKSLSERYREQSDLRLLVYIIVATLP
                     AVVIGVIFKDAIEAAFDHPRFVAWALIFTGLLLGLTRLSSGGKKELNFVNTLVMGLAQ
                     ALAILPGVSRSGSTISFGLFSGVRGEQAARFSFLLSIPAVLGATILKVGEMASAPVGS
                     GRLGLMIIGAIVSFFTGWLAIEAMLRILRRGKLYWFAPYCLLVGILMLFVL"
     gene            3251..3997
                     /locus_tag="PKM23_10845"
     CDS             3251..3997
                     /locus_tag="PKM23_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNY92010.1"
                     /translation="MKPPTIAFTMDQSPAADVRPFARGRDLYFINAAYVRYLEGADCL
                     PLALPTLLDHSRIPALIAGIDGLLLTGGDDVYAGAYGEQVIPGQWRIDPPRTFFEIEL
                     VREALRQHKPLFGICRGCQMINVARGGSLWQDIASQAPGARQHRSPESPILTYHPAAI
                     AAGSRLHAIVGRESLTVNSSHHQAIRKLASPLQITARAEDGIIEGIELPGEDFVLGVQ
                     WHPETMDEMSSSRALLEAFLANCLERSPAR"
     gene            3994..4995
                     /gene="holA"
                     /locus_tag="PKM23_10850"
     CDS             3994..4995
                     /gene="holA"
                     /locus_tag="PKM23_10850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNY92011.1"
                     /translation="MKTAIEQLHKEWNAGRFAPLYLFYGQDDYQIDLACAALVDKAVE
                     PGSEDFNFDQLYGGESDGAAIVNIAASYPMMAERRVLLVKNLHQLAAAGIELVARYAK
                     NPLPSTCLILTAAKIDARRSVWQQLIQSCSAVEFKPLYDNQVPEWLQRRISQSGLTIT
                     TEAIRLLQGLTGSSLRQLDSEIEKIRINLGERKEIGVEDVERVVGSSRQFSVFELCDA
                     VGQHRIGAGLAILDHMLTQGEQPTGILAMLARHFLILAKLHSMRGRRLPEESLAKALK
                     IQPFFLRNYSAQAGAFTGAQLSTALGHLLEADAQLKSSYQKPALVLELLLFKLRYAL"
     gene            5120..>5614
                     /locus_tag="PKM23_10855"
     CDS             5120..>5614
                     /locus_tag="PKM23_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNY92012.1"
                     /translation="MNKENPKLSQHSDEELIKRFQEGDLYAFDLIVHRYKDVLFNFTY
                     RYLGNSQEAEDVVQETFLRIFRNRFAYRQIAKFSTWIYTIAGNLAKTELRKRKRRRQV
                     YTSDMGFDDKEFEIEDTHADTEREVDSMLTEKHIQEAIDKLPERFRKVILLVDVQELS
                     YEEVS"
BASE COUNT         1124 a         1778 c         1623 g         1089 t
ORIGIN      
        1 gaactggtgc tcaaggattc ggagagcagc atccagcagg gcatccgggc gatgcaggag
       61 ctgctcgaca gccacgtcga tctggtcatt ggcgaactcg ccggcgaccg cagcgccgcc
      121 atggccgcgc tggcctcgga acgccgcatc ccgatgctgg tgccggtctc ctcggacaac
      181 ggcatcgccg ccctcggcac cggcgtctat cagatgacca gcgacctgga aacccgcggc
      241 gccgccctgg cacgctatgc cttcaacacc ctcggcctgc gcacctttgt ctcgctcgcc
      301 cccgccgatg attatggcca ggcgatgagc gacgcctttg ccaacaccat cgacaagctg
      361 ggcggcacca tcatcgccca gcagtggtat tttccgggga cccaggatgt ctcccgccaa
      421 ttcggcgcca tccgcgagtc agccagccag ttcattacgc cggatgccgg cgccatggca
      481 gccgaagcgg tcgactccgg gcagaacgaa aaagccgaat accgctatac ccgtcccacc
      541 cgccggccgg ctgcaacacc ggaagacgag gaggacatcc cccaactgcg ctcgatcgac
      601 ggttttttta tgccggccta tgcggaggac atctccatca tcgccccgca gttcagcctc
      661 gcccatatca aggccgtgcc cctcggcggg gacgactggc tccacggctc ggccctcaag
      721 agccagcggc gctatctgga cggtgcggtt ttctgcgccg gagcctttgc ggatgagacc
      781 ggcatggaat atatccagtt caagaatcgt tttagaatga cgacagggac aactgcaggc
      841 cctctggcta ttgccggcta tgatctcacg cacctgatca ccacagccat ccaggcgggc
      901 aaccgcaatg cggccggagt gatcgactgg ctcagcagga cgcagaagcg cagcggaatc
      961 gccaacggct attccttcgc gcccggtgtc agggtgaacc gcgatgtgat gatcctccag
     1021 tacaaagagg gcaacatctc ccgcctgctc tattgagccg gaaccgctgc aggccacttt
     1081 tacaggacaa aaggaggttt tgaaatggat gatgtcttga agaaagccat gatggccggt
     1141 ttgggaattt tctcgacgac caaggaaaag atcgaagagt tcgtcgagga gatgatccag
     1201 aagggtgaaa tggagcaggg cgacaaggcc aaggttgtac aggaaaccat ggacaaggtc
     1261 gaagcccgcg ccacggaagc caaggactgg atcgccaaac aggtcgagga gacttgggag
     1321 aagatgaaac ccaaggccca ggcccagctt gacgaactcc aggaaaaact ggccggtctt
     1381 tccagagagc tggaggagat gaaggcaaag atgggtccca aggagtagcc ccttaccgta
     1441 taagcacaaa tgaacagtat caaggaggca gtacgatgaa aaagctattg gtggtggtct
     1501 tctgcatgct ggtccctttc accatggcct tcgcgcagaa aacggatggt gtgcatctgt
     1561 tccagaattt tttcaatgat gccgtgatcg ccggcaatcc ctacggcgaa gccggtttgg
     1621 gttacgccac ttatgatggc ggcagcacga tcaccggcgg cgtccagggt ggtctgccgg
     1681 tcggcaagaa cctcgaactc aacgtcggcg tccactatct caactggagc tatgacaacg
     1741 acgatgccga ttcccagggc ggcctggccg atccctacgt tggacttcgc taccagctga
     1801 ctcctggccc gacccgtttc gcggtcggcg gctatgccac gcttcccgta ggcagcgagg
     1861 acatcggcca ggggcacctg aatttcggcg cttttggggc tgtccgccac aaactcgagg
     1921 ccttcaccct gaccgggacc gttggcatca atttttacga gtatactgat tacgaattcg
     1981 acatggatac cttcgaggtg gaggagagca ccgagcgcga caactatctc accctcggcg
     2041 ccggaatcat ctacccgacg agcaagaacc tggcgatcgt cggcgaatgg gtcctcaaga
     2101 ccgagatgga ctttatgatg ctgagcggcg gcgtggatta caatatgggc aacgtccgct
     2161 accgcggcgc tctgggcctc ggcctggatg acggcgcgcc tgatttccag atcctggcca
     2221 gctatctgct ccagttctag ctttcccctc tttccgtgaa aaaggccctc cccgagggcc
     2281 tttttcgtta tatatccctt gaatctcagc ttttttttcg ctaaactata gaattgattg
     2341 acccggagat ccggtccgga tggccttgcg gggccagaca cttgccgcgg gtcgggccgg
     2401 acaatacacc ttatggatga tgggaggcgc agtgaccctg ctcaattcga ttctcctcgg
     2461 catcgtgcaa ggattgaccg agtttctgcc ggtatccagc tccggccatc tggtgctctt
     2521 cagccatttc ctcggcgtcg aggacgccgg catcaccttc gaggtcctgc tccatttcgg
     2581 caccatcctg gccgtcctgg tcgctttccg cagggatatc ggccagctgc tcaccacctt
     2641 tttcggcctc ttccgcagcg gcaagagcct gagcgagcgc tatcgcgagc agagtgatct
     2701 tcgtctgctc gtctatatca tcgtcgccac cctgcccgcg gttgtcatcg gagtgatttt
     2761 caaggatgcc atcgaggccg cctttgacca tccccgcttt gtcgcctggg ccctgatctt
     2821 caccggcctc ctgctcgggc tgacacggct cagctctggc ggaaaaaaag aactcaattt
     2881 cgtcaacaca ttggtcatgg ggctggccca ggccctggcc attctgccgg gggtctcccg
     2941 gtcgggctcg acgatcagct tcggcctctt ctccggggtg cgcggcgaac aggcggcccg
     3001 cttttccttt ctgctctcca ttccggcggt gctgggcgcc accatcctca aggtcgggga
     3061 gatggcctcc gcaccggtcg gctcgggcag gcttgggctc atgatcatcg gggcaatagt
     3121 ctcctttttc accggctggc tcgccatcga ggccatgctg cgcatcctgc gccggggcaa
     3181 gctctactgg ttcgccccct actgcctgct ggtgggtata ctcatgctct tcgttctcta
     3241 ggcggacgcc atgaagcccc cgaccatcgc cttcaccatg gaccagagcc cggccgcgga
     3301 tgtgcgccct ttcgcccgcg ggcgggacct ctatttcatc aatgcggctt atgtccgcta
     3361 tctcgagggc gctgactgtc tgccgttggc cctgccgacc ctgctcgacc atagccggat
     3421 tccggccctc atcgccggga ttgacgggct gctcctcact ggcggcgacg acgtttacgc
     3481 cggggcgtac ggcgagcaag tcattcccgg tcagtggcgc atcgatccgc cccgcacctt
     3541 tttcgaaatc gaactcgtca gagaggccct gcggcagcac aagcccctct tcggcatctg
     3601 ccgcggctgt cagatgatca acgtcgcccg ggggggctcg ctctggcagg atatcgcctc
     3661 ccaggcgccg ggagcgcggc agcaccgctc gcccgagtcg cccatcctga cctatcaccc
     3721 ggcggccatt gccgcgggca gccgcctcca cgccatcgtc gggcgggaaa gccttacggt
     3781 caacagttcc caccaccagg cgatcaggaa actcgcctct cccctgcaga tcaccgcccg
     3841 cgcggaggac ggcatcatcg agggcatcga gctgcccggc gaggattttg tgctcggtgt
     3901 gcagtggcac cccgaaacca tggatgagat gagcagcagc cgcgccctgc tcgaggcctt
     3961 tctcgccaac tgcctggaga gaagcccggc acgatgaaaa ctgcaatcga acagctgcac
     4021 aaggaatgga acgcgggccg ctttgctccg ctctacctct tttacggcca ggacgattat
     4081 cagatcgatc tcgcctgcgc ggcgcttgtg gacaaggccg tcgagcccgg tagcgaggat
     4141 ttcaactttg accagctcta cggcggcgag agcgacggcg cggccatcgt caacatcgcc
     4201 gcctcctatc cgatgatggc ggaacggcgg gtgctgctgg tgaagaatct ccatcagctc
     4261 gcagctgcag ggattgagct ggtggccagg tacgccaaaa atcccctgcc gagcacctgc
     4321 ctgatcctca cggccgccaa aatcgatgcg cgccgttcgg tctggcagca gctcatccaa
     4381 agctgcagcg ctgtggagtt caaacccctc tacgacaatc aggtgccgga gtggctgcag
     4441 cggcgcatca gccagtcggg gctcaccatc acaacagaag cgatccgcct gctgcagggc
     4501 ttgaccggca gcagcctgcg ccagctcgac agcgaaatag aaaaaatcag aatcaatctc
     4561 ggggaacgaa aggaaatcgg agtggaagac gtggagcggg tggtcggctc ctcgcgccag
     4621 ttctcggtct ttgaactctg cgatgcggtc ggccagcaca ggatcggcgc cggcctggcc
     4681 atcctggacc atatgctcac ccagggggaa cagcccaccg gcatcctcgc catgctggcg
     4741 cgccatttcc tcattctggc caagctgcac agcatgcgcg gccggcgcct ccccgaggag
     4801 agtcttgcaa aagccctgaa aatccagccc tttttcctga gaaactattc agcccaggcc
     4861 ggcgccttca ccggcgccca gttgagcacc gccctgggac atcttctcga ggccgacgcc
     4921 cagctcaaga gcagctacca gaagccggcc ctggtgctcg aactgctgct gttcaaattg
     4981 cgctatgccc tctgagatcg aatccgcctt gcgcggattg atagaggcgg ccctccgctc
     5041 cgggaaaaca tcccggctcc agcgcaggga tttggtcggg aacttttgcc gccccgtgcg
     5101 gtttcaagac tagaaccgga tgaacaaaga gaatcccaaa ctctcccaac acagcgatga
     5161 agagctcatc aagcgattcc aggagggtga tctatacgcc tttgatctga tcgtgcatcg
     5221 ctacaaggat gtgctgttca actttaccta tcgatatctt ggcaattccc aggaagccga
     5281 ggatgtggtt caggagactt tcctgcgcat ctttcgcaac cggttcgcct accggcagat
     5341 tgccaagttc tcgacctgga tctatactat cgccggcaat ctggccaaaa ccgagctgcg
     5401 caagcgcaag cggcgccggc aggtctacac ctcggacatg ggattcgacg acaaggaatt
     5461 cgagatcgag gacacccacg ccgatacgga acgcgaggtg gacagcatgc tgaccgagaa
     5521 gcatatccag gaggcaattg acaagcttcc ggagcgcttc cgcaaggtca ttctgctcgt
     5581 ggatgtccag gaactttcct atgaggaagt cagt
//
LOCUS       DAOPWU010000224         5598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14447_length_5598_cov_4.373985, whole genome shotgun sequence.
ACCESSION   DAOPWU010000224 DAOPWU010000000
VERSION     DAOPWU010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14447_length_5598_cov_4.373985"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PKM23_10860"
     CDS             complement(<1..221)
                     /locus_tag="PKM23_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY92013.1"
                     /translation="MPIKVLVVDDSALIRALLKEIIQADPELELVGQAPDAYVARDLI
                     KQLNPDVLTLDVEMPRMDGLTFLEKLMRGR"
     gene            240..1709
                     /locus_tag="PKM23_10865"
     CDS             240..1709
                     /locus_tag="PKM23_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNY92014.1"
                     /translation="MDLAHHRGMKVLLDMIFNHVAPDHPWVVDADLWEGAGYKNWFHP
                     HSGVDGSTSIEDWQDQQQLEERELNGLPDLAQENPHVYNFLLDMAKFWIVESGCDGFR
                     LDAVKHIPPAFWSRICRDLHDFAGEDFLLLGEVFAGETPYVAGYQDLGFNALFDIPLY
                     YTLNRVFAQGGTPPLLTRQTALNRQWYRTILLSPVIDNHDVARFSYWAGDHAAAKMRS
                     ALTFTLVQPGLPMLYYGNEVALEGAAATNEQTGAGQDYLNRLPMPWDRLVDSSRDLVE
                     HTRRLLHLRKQLPALAGAGLTEIYQDYGIYAFIKHRGREAVLVVLSNSGESEETAIPL
                     PKGLFTRCTRWEDQLSGLRLKARRDTLHLSLPPRANHLFRIKGRWHPDLLASLPWSCS
                     FTPRISRDMEWIDFYYQGEARSVSVAGDFNGWIAGMDSLRRDGDGLWQARLPLKKGRY
                     RYKMVIDGSRWIADPAARESELDPYGGRNSVVVVGGAVQ"
     gene            complement(1877..5389)
                     /locus_tag="PKM23_10870"
     CDS             complement(1877..5389)
                     /locus_tag="PKM23_10870"
                     /inference="COORDINATES: protein motif:HMM:NF020698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNY92015.1"
                     /translation="MHLKRFTLLYLIAVSLFTILETPSLGQPWHTQVLYHDNLDNPNS
                     MALGAMQNTDGVFISGRGWQAVASTSKLFITLPADLPWEGSMEVDVTNFDPAEQADDP
                     EIKYHCINIWSRPEADKDAYETDASWTQIRTSGHDKYNDGDGRAGFKWLTQTHGSGDD
                     SREEDYYLLNKDWDKSKTYTFRIVWTTDRAYLYLDNLLLGDYYRNRTYHPFSGRLEPF
                     RYIFLGKENLIWGYEAQVGPIYSNLTIYGPDGSTPPPPPPPPPPPDTTEVPDPSGKGF
                     TDITASSLTGGYSASGYGQGSSFGDIDKDGWMDLFVSNGTSYAPMPDLLYINEGNGTF
                     SEEAAARGTQSRGLTKAILLADFDNDADLDAFFGNVPLEAGDPAGINALYFNNGSGYF
                     NEGGAAAGLVQTPKTSNGSVALDMEGDGDLDLFVVTSGELNELYENDGSGRFTLVQRG
                     AEGSVEDTQYFGRQGATAGDVDNDGDIDIYICRRQESTSQAAPNWLFINDGSGRFTEE
                     AAARNAAINGRSHGATFADIDNDGDLDLFVVNYLLTTGNVPLLNVLINDGTGHFIDRS
                     STYNLPISGYTVAFGDMDNDADLDLLLLRNGEKDAGARPQLYLNDGDGRFSLAADSGV
                     EVAAADARSAALADVDRDGDLDIYMASAKGANFLVRNDLDNDYHWLDLLCTGPKGDLG
                     GIGSKVILYEAGRLGEAAYIIGYQEVGSQFGYMSQNPVQLHFGLGTRTSCDVRVILID
                     QQTIDIPGAAADQQLRPAPPEPARIPTMSKGSGEGQVLHPGQEFPEDFTARIQDQHGQ
                     PVVNQAVLFEVLAGGGHLRGETSLEIFSDDEGYASVHWTPGPWKGVANVLKASAWLDS
                     VEIANSPLTFTYPAADLSAALSSVDAATPIHADGSTRSDIVVTLRSSAGQPLGAGYSV
                     ELLVSGSGYVVTVADTLTDAEGRVAATLTATQPGECVVRARVKGIGLTLADSALITVL
                     PVVEPNQPPQIFTFFPSENPVYGQFYTPITFALTTVRDADGDSLRYLWRINEVVASTD
                     SQMVLYPSIEMGAVYEVTALVMDEEDTARVDWRVLVALTGVAEEAALPTVLKLQQNYP
                     NPFNPSTTIKVEVPESMEATLGIFNTRGQRVRLLHEGLLAAGVRSWNWDARDDSGLPL
                     PSGVYHAVLRRGQEVQIRRLLFMK"
BASE COUNT         1034 a         1706 c         1782 g         1076 t
ORIGIN      
        1 cggccgcgca tgagtttctc cagaaaggtc aggccgtcca ttcgcggcat ttccacatcc
       61 agggtcagca cgtcagggtt cagctgcttg atcaaatcac gggccacata ggcatccggc
      121 gcctggccga ccagctccag ctcggggtcg gcctggatga tctccttgag cagcgcccgg
      181 atcagcgccg agtcgtctac caccaacact ttgattggca tacctgccct ctagaaaaga
      241 tcgatctcgc ccaccaccgc ggcatgaagg tcttgctcga tatgatcttc aaccatgtcg
      301 caccggacca tccctgggtc gtcgatgcgg acctctggga gggggccggc tacaaaaact
      361 ggttccatcc tcacagcggc gtggacggtt ccaccagcat cgaggattgg caggatcagc
      421 agcagctcga ggaacgagaa ctcaacgggc tgcctgacct ggcccaggag aatccccatg
      481 tctataattt tcttctcgac atggccaaat tctggattgt cgagagcggc tgcgacggct
      541 tccgccttga tgcggtcaag cacatccccc ccgccttctg gtcgaggatc tgccgggatc
      601 tgcacgactt tgccggtgaa gattttcttc ttctcggcga ggttttcgcc ggcgaaaccc
      661 cctatgtggc cggctaccag gatctgggtt tcaacgccct cttcgatatc ccgctctatt
      721 acaccctcaa ccgcgtcttc gcccaggggg gcacaccgcc gctgctgacc cgccagaccg
      781 cgctcaaccg ccaatggtac cgcaccattc ttctctcccc ggtcatcgac aatcacgatg
      841 tcgcccgctt cagctactgg gcgggcgacc atgccgccgc caagatgcgt tcagccttga
      901 cctttaccct ggtccagccc ggcctgccca tgctctatta cggcaacgag gtcgcactgg
      961 agggggccgc cgcgaccaac gagcagaccg gcgccggaca ggattatctc aaccgcctgc
     1021 ccatgccctg ggatcggctg gtggactcga gccgggatct ggtggaacat acccgccgcc
     1081 tcctgcatct gcgcaaacaa ctgcccgccc tggccggagc agggctgacc gagatctatc
     1141 aggattacgg catctatgcc tttatcaagc acaggggcag ggaggccgtt ctggttgtgc
     1201 tctccaactc cggcgagtct gaagaaacgg ccatccccct gcccaaaggc ctcttcaccc
     1261 gctgtacgcg ctgggaggac cagctctccg gcctgcgcct caaggcccgc agagacaccc
     1321 tccacctctc cctgccgccg cgggcaaacc acctcttccg catcaaaggc cgatggcatc
     1381 ctgacctgct ggcttctttg ccctggtcct gctccttcac gccgcgcatt agcagggata
     1441 tggaatggat cgatttttat tatcaggggg aggcccgctc tgtcagcgta gccggggatt
     1501 ttaacggctg gattgccggc atggacagcc tgcgcaggga tggggatggc ctctggcagg
     1561 cacggctgcc cctcaaaaag ggacgctacc gctacaagat ggtcatcgac ggcagccgct
     1621 ggatcgccga tcccgccgcc cgcgaaagcg agctggatcc ctatgggggg cggaattcgg
     1681 ttgtcgtcgt cggaggggca gtgcagtgat cagaaccccg gccggcgccg gagcggccgg
     1741 tcctggttcc gctatgggcc gtccccccag gcacggcagc acaccggatg gccgcctttg
     1801 ccgcaccgcc aactggccat aaaaaaggcc gggctataag cccggccttt tttatggtgg
     1861 attgtctagg ccgcatctat ttcataaaca gcagccgccg gatttgcact tcctgccccc
     1921 ggcgcagcac cgcgtgatag accccgctcg gcaagggaag gccggagtca tcccgggcat
     1981 cccagttcca gcttctcacc cccgcagcca gtagcccctc atggagaagg cgcaccctct
     2041 gaccgcgcgt gttaaaaata ccgagggtgg cctccatgct ctccgggacc tcgaccttga
     2101 tggtcgtgga gggattgaaa ggattgggat agttctgctg cagcttcaga acagtgggca
     2161 gggctgcttc ctcggccacc ccggtgaggg cgaccagcac ccgccagtca acccgggcgg
     2221 tatcctcctc atccatgacc agggcggtga cctcataaac cgcccccatc tcgatcgagg
     2281 gatagagcac catctggctg tcggtcgagg cgacaacctc gttgatgcgc cagagatagc
     2341 gcaaagagtc cccgtcggcg tcgcggacgg tggtcagcgc aaaggtgatg ggggtataga
     2401 actggccgta gaccggattc tcgctcggga aaaaggtaaa gatctgcggc ggctggttgg
     2461 gctccaccac cggcaggacg gtaatcagcg cactgtcggc cagggtgagg ccgatgccct
     2521 tgacccgggc gcgaaccacg cactcgcccg gctgggtggc agtgagagtg gcggcgacgc
     2581 gcccctcggc atcggtcagg gtatcggcga cagtgacgac atagccgctg ccgctcacca
     2641 gcagttcgac cgaatagccg gcgccgaggg gctgcccggc actgctgcgc aaggtcacca
     2701 cgatatcaga ccgggtggat ccgtccgcat ggatgggcgt ggcggcatcg acactggaga
     2761 gggctgcact caggtcggcg gccgggtagg tgaaggtgag cggagagttg gcgatctcca
     2821 cgctgtccag ccaggcggaa gccttgagca cattggcgac ccccttccag gggccggggg
     2881 tccagtgcac gctggcatag ccctcgtcat ctgaaaagat ctcgaggctg gtctcgccgc
     2941 ggagatggcc gccgcccgcg agcacctcaa aaagcaccgc ctggttgacg acgggctggc
     3001 catgctgatc ctggatgcgg gcggtgaaat cctcggggaa ctcctgaccc ggatggagta
     3061 cctggccttc gcccgatccc ttgctcatgg tgggaatgcg tgccggctcc ggaggggcgg
     3121 gacgcagctg ctggtcagcg gccgcgccgg gaatatcgat cgtctgctgg tcaatgagga
     3181 tgacgcgcac atcgcagctg gtgcgggtgc cgagcccgaa atggagctgg accggattct
     3241 ggctcatgta gccgaactgg ctgcccacct cctggtaacc gatgatatag gcggcttcac
     3301 cgaggcggcc ggcttcgtag aggatcacct tcgaaccgat gccgcccaga tctccttttg
     3361 gtcccgtgca gaggaggtcg agccagtgat agtcgttgtc gaggtcgttg cgcacgagga
     3421 aattggcccc cttggcgctg gccatataga tgtcgagatc tccatcccgg tcgacatcgg
     3481 ccagggcggc gctgcgcgca tcggcggcgg cgacctccac cccggaatca gcggccaggc
     3541 tgaaccggcc gtctccgtca ttcaggtaga gctggggtct ggcgccggcg tccttctccc
     3601 cgttgcgcag aagcaggaga tcgaggtcgg cgtcgttgtc catatccccg aaagccacgg
     3661 tatagccgct gataggcagg ttataggtcg atgagcggtc gataaaatgg ccggtgccgt
     3721 cgttgatgag gacgttgagc aggggtacat tgcccgtggt cagaaggtag ttaacgacga
     3781 agagatcgag atcgccgtca ttgtcaatgt ccgcgaaggt ggcgccgtgg gagcggccgt
     3841 tgatcgcagc attgcgcgcc gccgcctctt cggtgaagcg gccgctgcca tcattgatga
     3901 aaagccagtt gggtgccgcc tggctggtcg actcctggcg gcggcagata tagatatcga
     3961 tatcgccgtc attgtcaaca tcgccggcgg tcgcgccctg gcgcccgaaa tactgggtat
     4021 cctcgacgct gccctcggcc ccgcgctgaa cgagggtgaa ccggccgctg ccgtcgttct
     4081 cataaagctc gtttaactcg ccggaggtga cgacgaagag gtcaaggtcg ccatccccct
     4141 ccatatccag ggccacgctg ccgttggagg ttttcggggt ctgcaccagt ccggccgcag
     4201 ctccgccctc gttgaagtag ccgctgccat tattaaagta gagggcgttg atgccggccg
     4261 gatcgccggc ctcgaggggg acattgccga aaaaggcgtc caggtccgca tcgttgtcaa
     4321 aatcggcgag caggatcgcc ttggtcagtc ccctgctttg ggtcccacgc gcggccgcct
     4381 cttcgctgaa ggtgccattg ccctcgttta tgtaaagaag atcgggcatg ggagcgtagc
     4441 tggtgccgtt gctaacaaag agatccatcc agccgtcctt gtcgatgtcg ccgaaggagc
     4501 tgccctgacc gtagcccgag gcggagtagc cgccggtgag ggaagaggcg gtgatatcag
     4561 taaagccctt gcccgaggga tcgggcactt cggtggtgtc ggggggcggc ggtggaggcg
     4621 gaggcggcgg tggggtgctg ccatccggcc cgtaaatggt caggttggag tagattggtc
     4681 cgacctgggc ctcatatccc cagatcaggt tctccttgcc gagaaagatg tagcggaagg
     4741 gttcgaggcg gccggagaag gggtgatagg tgcggtttct gtaataatcg ccgagaagga
     4801 gattgtccag gtagaggtag gcccggtccg tcgtccagac gatgcggaag gtataggttt
     4861 ttgatttatc ccagtccttg ttgagaaggt aatagtcctc ctcgcgggaa tcatcgccgg
     4921 agccgtgggt ctgggtgagc catttgaagc cggcgcgccc gtcgccgtca ttatacttgt
     4981 cgtgcccgga ggtacggatc tgggtccacg aagcatcggt ctcataggca tccttgtccg
     5041 cctccggccg ggaccagatg ttgatgcagt gatatttgat ctccgggtca tcggcctgtt
     5101 ccgccgggtc gaaattggtc acgtcgacct ccatggagcc ctcccagggc agatcggcgg
     5161 gcagggtgat gaagagcttg ctggtggagg cgactgcctg ccaccccctg cccgagatga
     5221 agaccccgtc cgtgttctgc atggcgccga gggccatgga gttgggattg tccaggttgt
     5281 cgtgataaag cacctgggtg tgccagggct gcccgagcga gggtgtctcc agaatggtaa
     5341 aaagggatac agcgatcagg tacaacagtg taaagcgttt taaatgcatg acgggatctc
     5401 ctgcgcatga tgcatggctc aaaattatcc cccacgactg cgccggtccg gggccggatt
     5461 gccgggcgcc attccggtat ctattagtac cgccgcctcg gttttttgcg taagaaagag
     5521 ttgctgttct gtgcggggga tcacacggcc acaaggtggg attcaaaccc tccgcgccca
     5581 gaattactgg gcggggag
//
LOCUS       DAOPWU010000225         5589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14477_length_5589_cov_3.838995, whole genome shotgun sequence.
ACCESSION   DAOPWU010000225 DAOPWU010000000
VERSION     DAOPWU010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14477_length_5589_cov_3.838995"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKM23_10875"
     CDS             complement(<1..373)
                     /locus_tag="PKM23_10875"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNY92016.1"
                     /translation="MKIAPKVQLICAVCCRDEESSVQACERLTQLCGPISSRTKPFAF
                     IHTRYYQKEMGDRLQKFFCAFSRPVDPMQIVGIKLATNRIEEELAWEGRRRVNLDPGS
                     IEAAELVLATTKNFGHRIYLGQ"
     gene            complement(422..1072)
                     /locus_tag="PKM23_10880"
     CDS             complement(422..1072)
                     /locus_tag="PKM23_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92017.1"
                     /translation="MRLSAWPIRIVMILLFFSLTATAQVNSSSIIEAKVYPDSSKPAV
                     TVKNLQLEFVFAGYGVYLPSDYPTVTHFPLESGERVLMENVAEITCFPMRVEWKKYLE
                     PDQRSRSVPVDAQGYRHWSDVEVEVLIKDWNGNQVKSRLFRPEISDVYLIGRTDRGDF
                     RLQLDQENGKTVRIEFEPLFIMQCSGDINHLFPNMKWKYCPLCGASLKKVTKKKGM"
     gene            complement(1239..2498)
                     /locus_tag="PKM23_10885"
     CDS             complement(1239..2498)
                     /locus_tag="PKM23_10885"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNY92018.1"
                     /translation="MEKADVFAMALEQLDSAARLLKLDANTHRVLAHAERALTVALPV
                     VMDDGHIEVFEGHRVQHSSARGPCKGGIRFHQNVTLDEVTALSMWMTWKCATVNIPYG
                     GGKGGVKVDPTKLSKDEIRHLTRRYTVAIMPIIGPHSDIPAPDVNTSAETMGWIMDTV
                     SMFRGSTVLDIVTGKSIELGGSLGRREATGRGVMFNTIELLKRLDKDPAKTRVVVQGF
                     GNVGSVSADLLQKRGCKIIAVSDVSGAYYNPAGLPISDMIRYCSTSKNRLLEGFDAPG
                     ISKVSNAELLELDTDILVPAALEKQITIENADRIKAYAIVEGANGPTTPEAERLLVSK
                     GKYVVPDILANSGGVVVSYFEWVQSIQSFFWTEEEVNKNLERVIENAFAAVWDLARRE
                     KQDLRNGAMMIAVDRVAKAVQSRGIWP"
     gene            complement(2836..3261)
                     /locus_tag="PKM23_10890"
     CDS             complement(2836..3261)
                     /locus_tag="PKM23_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNY92019.1"
                     /translation="MESNPVPIMATQRLDKWLKIARLFKTRSQATEACDERRVKVNGQ
                     VAKPAKEIRIGDTLTIRHHGGTYIELEVVQLCSKSIAAALARQLYTLHVRETTPEEQE
                     LMKLFSEAVKRVRPAYKGRPTKKERRRLEQHRRDSNPWR"
     gene            complement(3251..5239)
                     /gene="ftsH"
                     /locus_tag="PKM23_10895"
     CDS             complement(3251..5239)
                     /gene="ftsH"
                     /locus_tag="PKM23_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNY92020.1"
                     /translation="MNLLHNENRNSNRHKPRMEKKLHFSIWYVLFAIMTLFLFNDLFF
                     SSHTQEVPYSTFKELLRQSRVFEAGVSTDRIEGFYFIGDSTNLAARRARYDSLQATAS
                     RRSGFNIREIFTSAKDRELFVEKNLQSFFTRRIDDGDLVKELEAYRVAYRGVEGEDWL
                     RSLILGWILPFAILFAIWGYIFKRMNPSGGMMSIGKSKAKIYVEGQTKVTFKDVAGID
                     EALEEVSEIVEFLKNPEKFQRLGGRIPKGVLLVGPPGTGKTLLAKAVAGEAQVPFFSI
                     SGSDFVEMFVGVGAARVRDLFQQASQKAPCIIFIDELDALGKARGVNVYGGHDEREQT
                     LNQLLSEMDGFESNTGVIIMAATNRPEILDIALLRPGRFDRQIVVDRPDINGRESILR
                     VHARQVKLAREVDLKVIAARTPGLVGADLANLLNEAALIAARHNQKRVEMHDLEEAID
                     RVIAGLEKKSRVMNKREKETVAYHEAGHAIVASAVPGSDPVHRVSIIPRGVAALGYTL
                     QLPTEDRYLMTRSELIGRITVLLGGRVAEELKYGQVSTGGQNDLERATDIARSMVAEY
                     GMSDQIGPVSYGRGGRTLFLGAEIPESRVYSESLAAKIDAEIRILLEEAETRVHTILN
                     QKRQQLETLAQRLLEKELVEKNELAAILDGNGAKNDGK"
     gene            complement(5281..>5589)
                     /locus_tag="PKM23_10900"
     CDS             complement(5281..>5589)
                     /locus_tag="PKM23_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNY92021.1"
                     /translation="MNQRGQIKGCIIDGPLALDNAISAEAAALKGIESPVAGKADILL
                     CPEIESANMLAKSTTYFARMRLGHVIMGSTAPVLIPSRADSTDSKLLSIAIGKLICTR
                     "
BASE COUNT         1115 a         1556 c         1691 g         1227 t
ORIGIN      
        1 cctggccgag atagatgcgg tggccgaaat tcttggtggt tgccagcacc agctcggccg
       61 cctcgataga gcccggatcg aggttgacgc gccggcgccc ctcccaggcc agctcctcct
      121 cgatccgatt ggtggcgagt ttgatgccga caatctgcat cggatcgacg ggacgactga
      181 aggcgcagaa gaacttttgc agccggtcac ccatctcctt ctggtaatag cgggtatgga
      241 tgaaggcgaa gggtttggtg cgcgaagaga tcggcccgca aagctgcgtg agtcgctcgc
      301 aggcctgcac gctgctctcc tcgtcccggc agcagactgc gcatatcagc tgcaccttag
      361 gtgcaatttt cacctggccc atcaggatac tccgcgggga caatgatcgg ttgtgcccgg
      421 ctcacatccc cttcttcttg gtcactttct tcagcgaagc gccgcaaaga ggacaatatt
      481 tccacttcat gttgggaaag aggtggttga tgtcacccga gcactgcatg atgaagaggg
      541 gttcaaactc gatgcgaacg gttttaccat tttcctggtc cagctgcagg cgaaaatcgc
      601 cgcgatcggt ccggccaatg aggtaaacat ccgagatttc cggcctgaag aggcgcgatt
      661 tcacctggtt gccattccag tccttgatga gcacctcaac ctcgacgtcg ctccagtggc
      721 ggtatccctg ggcatcgacc ggcacgctgc ggctgcgctg gtcgggttca agatatttct
      781 tccactccac gcgcatgggg aagcaggtga tttcagccac attttccatc aacacgcgct
      841 cgccggattc gagcggaaaa tgggttaccg tcgggtaatc tgaaggcaga tagaccccat
      901 agccggcgaa aacaaactca agctgtagat tcttgacggt taccgctggt ttcgaagagt
      961 ccggatagac cttggcctcg atgatcgatg aggagttgac ctgtgccgtg gcggtgaggc
     1021 tgaagaaaag aaggatcatg acgatccgta tgggccacgc cgaaagtctc atggcggcct
     1081 cctttctgcc ccgatgctga agttatggta aatattgaac atccctccga agcggaggaa
     1141 ggaacggatt atatatccta ttaaatttat tatatttgtt ataaaatatc aagaaaaaaa
     1201 agcccccttg cgggggcttt gtctggatga ccacaagatc acggccagat accgcgggac
     1261 tggaccgcct tggccacccg gtccacggcg atcatcatcg ctccgttgcg gagatcctgt
     1321 ttttcacgcc tggcaagatc ccagacagcc gcgaaggcat tctcgatgac gcgttccagg
     1381 tttttgttga cctcctcctc agtccagaag aaggactgga tcgactgcac ccattcgaaa
     1441 taggagacga ccacgccgcc gctgttggcg aggatatcgg gaacgacgta cttgcccttg
     1501 ctgaccagca ggcgttcggc ttcgggggtt gtcggtccgt tggccccttc gacgatggcg
     1561 taagccttga tccggtcggc attttcgatc gtgatctgct tctccagcgc agcaggaacc
     1621 aggatatcgg tatcgagttc gagcagctcg gcattggaga ctttgctgat gccgggggca
     1681 tcgaagccct cgagcagccg gtttttcgaa gttgaacagt agcggatcat gtcgctgatt
     1741 ggcagaccgg ccggattgta ataggcgccg ctgacgtcgc tgacggcgat gatcttgcag
     1801 ccccttttct ggaggaggtc ggcgctgacg ctgccgacat taccaaagcc ctggacgacg
     1861 acgcgggtct tggccggatc cttgtccagc cgcttgagca gttcgatcgt gttgaacatg
     1921 acgccgcggc cggtcgcctc gcgccgcccg agtgagccgc cgagttcgat cgacttgccg
     1981 gtgacgatat cgagcaccgt gctgccgcgg aacatgctga ccgtatccat gatccagccc
     2041 atggtctcgg cgctggtatt gacatcggga gcgggaatgt cgctgtgcgg gccgatgatg
     2101 ggcatgatcg cgacggtgta gcgccgggtg aggtggcgga tctcatcctt gctcaacttg
     2161 gtcggatcga ccttgactcc gcccttgccg ccgccgtagg ggatattgac cgtggcgcat
     2221 ttccaggtca tccacatcga cagcgcggtc acctcatcga gggttacgtt ctgatgaaag
     2281 cggataccac ccttgcaggg accgcgggcg ctcgaatgct gcacgcggtg accttcgaaa
     2341 acctcgatgt gaccatcatc catgacgacc ggaagtgcga cagtcaaggc ccgctctgcg
     2401 tgggcgagaa cgcgatgcgt attggcatcg agtttgagca gccgcgcagc ggagtcgagc
     2461 tgttccagtg ccatggcgaa tacatcagct ttttccataa tacactccca tcatttaaga
     2521 cagctgtacc gcttgttggt ttagagtgaa acctcaattt acagagaaaa ataaagccct
     2581 gttccaacac ttggaagagg gctataataa aacggttaaa aggatcaggc accagatcga
     2641 gcaaatcggg caccccggca taaagccggc tttcaggatg cctgtaaaat tcggtatggg
     2701 gtactttaaa ggcataaggg gaattgataa ctgcaatagg ccatttggat gacaaccggg
     2761 atgccaggca tcctgtaaag cgctggctaa tgtaggtaat ataaaaatga ttgtcaaggg
     2821 ataaatcgcc ctccgtcacc gccagggatt gctgtcgcgg cggtgctgct cgagtctgcg
     2881 gcgttccttc ttggtgggcc tgcccttgta ggctggcctg acccgtttga ccgcctcgct
     2941 gaagagcttc atcagctcct gctcctccgg ggtggtctcg cgaacatgca gggtgtagag
     3001 ctggcgggcc agggcggcgg cgatgctttt gctgcagagc tgcacgacct ccagttcgat
     3061 ataggtgccg ccgtggtggc ggatagtcaa ggtgtccccg atgcggatct ccttggctgg
     3121 tttggcgacc tggccgttga ccttgacccg acgctcatca caggcttcag tcgcctggga
     3181 gcgggtcttg aacagacggg cgattttcag ccatttgtcc agccgctgcg tcgccataat
     3241 ggggacggga ttactttcca tcgttcttcg ctccgtttcc atccaggatg gcggccagct
     3301 catttttctc caccagctct ttttccagca gtctctgggc gagggtttca agctgctgcc
     3361 gcttctggtt gaggatggtg tggacccggg tttcagcctc ctcgaggagg attctgatct
     3421 cggcatcgat tttggccgcc aggctttcgc tgtagaccct gctctcgggg atctcggcgc
     3481 ccaggaagag cgtgcggccg ccgcgtccgt aggagaccgg accgatctgg tcgctcatcc
     3541 cgtattcggc caccatgctg cgggcgatat cggtggcgcg ctcgaggtca ttctgtcccc
     3601 cggtcgagac ctggccgtac ttgagttctt cggcaacccg gccgccgagc aggaccgtga
     3661 tcctgccgat cagttcggaa cgggtcatga gatagcggtc ctcggtcggg agctgcaggg
     3721 tgtagccaag cgcggcaact ccgcgcggga tgatcgacac gcggtggacg ggatcgctgc
     3781 ccgggacggc cgaggcgacg atggcatggc cggcttcatg ataggcgacg gtctctttct
     3841 cccgtttgtt catcacccgg ctcttcttct caaggccggc gatgacccgg tcgatggcct
     3901 cctccagatc atgcatctcc accctcttct ggttgtggcg ggcggcgatg agcgcggctt
     3961 cgttgaggag gttggcgaga tcagccccga ccaagcccgg ggttctggcg gcgatcacct
     4021 ttaaatcgac ctcgcgggcg agcttgacct gacgggcatg gaccctgagg atcgactccc
     4081 ggccgttgat atcggggcgg tcgacgacga tctggcggtc gaagcgcccg ggccggagca
     4141 gggcgatgtc gaggatttca gggcggttgg tggcagccat gatgatgacg ccggtattgg
     4201 actcaaaacc atccatttcg gagaggagct gattgagggt ctgctcgcgc tcgtcgtgcc
     4261 ccccatagac attgacgccg cgggccttgc caagggcatc gagttcatcg atgaagatga
     4321 tgcagggggc tttttgcgag gcctgctgaa agaggtcgcg gacgcgcgcg gctccgacgc
     4381 cgacgaacat ctcgacgaaa tcggagccgg atatgctgaa aaaaggaacc tgagcctctc
     4441 ctgccacggc cttggcaagc agggtcttgc cggtgcccgg gggtcctacc agaagaaccc
     4501 cttttggaat ccggccgccg aggcgctgga atttctccgg atttttaaga aactcgacga
     4561 tttcgctcac ctcttcaaga gcttcgtcga tgccggcgac atccttgaaa gtgaccttgg
     4621 tttgcccctc gacgtagatc ttggccttgc tcttgccgat ggacatcatg ccaccgctgg
     4681 gattcatgcg tttgaaaata tagccccaga tggcgaagag gatggcgaag ggcagaatcc
     4741 agcccagaat cagggagcgc agccagtcct ctccctccac cccgcggtaa gcgacgcgat
     4801 aggcttccag ttccttgacc agatcgccgt catcgatgcg ccgggtgaaa aaagactgga
     4861 gattcttctc cacgaagagt tccctgtcct tggcggaggt aaaaatctcc cggatattaa
     4921 aaccgctgcg ccgcgaagcc gtcgcctgca gagaatcata acgggcacgg cgtgcggcca
     4981 ggttggtgga atcgccgatg aaatagaatc cctcaattct gtcggtcgaa accccggctt
     5041 caaaaacccg gctctgtcgg agaagctcct tgaaggtgct gtagggtacc tcctgtgtat
     5101 gggaggagaa gaagaggtcg ttgaagagga agagagtcat gatggcaaaa agcacatacc
     5161 agatggaaaa atggagtttt ttctccattc tgggtttatg gcgattggaa ttcctgttct
     5221 cgttgtgcag caaattcatg cagtactcct gctcttgcgc cccgccctct cagccgggcc
     5281 ctatctggta caaatcagct tgccgatggc gatggagagc agtttgctgt ccgtgctgtc
     5341 ggcgcgtgag gggatcagca ccggtgcggt cgatcccata atgacgtggc ccaggcgcat
     5401 gcgggcgaaa taggtggtcg atttggccag catgttggcc gattcgatct ccggacagag
     5461 caggatatca gcctttccag ccacagggga ttcgattccc ttgagcgctg cggcttcggc
     5521 ggagatcgca ttgtcgagcg cgaggggtcc atcgatgatg cagcccttga tctggccgcg
     5581 ctggttcat
//
LOCUS       DAOPWU010000226         5584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14492_length_5584_cov_3.861096, whole genome shotgun sequence.
ACCESSION   DAOPWU010000226 DAOPWU010000000
VERSION     DAOPWU010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14492_length_5584_cov_3.861096"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKM23_10905"
     CDS             complement(<1..386)
                     /locus_tag="PKM23_10905"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610628.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNY92022.1"
                     /translation="MFTILVINPGSTSTKIALFRDENPLFTASLPHSSEELASFPTIY
                     SQFAFRRQAILKILEEKNVKIEEIDAFVGRGGLLHPVTSGTFRVGPAMLTQLEKADFG
                     EHASNLGAILAHELAAMYGRPAFIVDP"
     gene            complement(397..918)
                     /locus_tag="PKM23_10910"
     CDS             complement(397..918)
                     /locus_tag="PKM23_10910"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017327840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNY92023.1"
                     /translation="MDLAARIRSIPDFPKPGIVFKDITTLLGDGPALRETMAELRKRF
                     DGETVDAVIGIESRGFIFAAILAHELGVGMVPVRKPGKLPFTTIAESYALEYGSDSLE
                     MHVDALKPGQRVVIVDDLLATGGTVEAALRLARKLGAEVIGCAFVIELDFLGARQRLA
                     PMRLESLIHVESE"
     gene            complement(1011..2303)
                     /gene="asnS"
                     /locus_tag="PKM23_10915"
     CDS             complement(1011..2303)
                     /gene="asnS"
                     /locus_tag="PKM23_10915"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNY92024.1"
                     /translation="MPTVYIDEIAKFQGQEVTLQGWVYNKTAKGKLIFLMLRDGTGLI
                     QAVMFRDTVGDALFESTLALTQESSVRITGTVSEDKRAKGGFELQVSTLEVVHLAAEY
                     PISPKEHGIDFLMEHRHLWLRSSLQHAVLKVRHEIISACRDYFDNMNFTLIDAPIFTP
                     AAAEGTSTLFETNYFEDKAYLTQSGQLYMEAAAMAFGKVYCFGPTFRAEKSKTRRHLT
                     EFWMIEPEVAFCDLEGDMELAEGLICAIVERVLDKRREELKRLERDLSKLECIRKPFP
                     RLDYEDAAQILKEENSQFVYGDDLGAPDETIISARYDRPVMVTHYPAGVKAFYMKRDP
                     ERPDRALCVDLLAPEGYGEIIGGGQREDDLDTLLSRIHEHQLPAENYDWYLDLRKFGS
                     VPHAGFGLGLERTVTWICGLQHIRETIPFPRTISRIYP"
     gene            complement(2354..3190)
                     /locus_tag="PKM23_10920"
     CDS             complement(2354..3190)
                     /locus_tag="PKM23_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNY92025.1"
                     /translation="MFKYILLAVDGSAYTDSVLSHGIELAGRFGSRLNLLTVADVRIF
                     EWASAVGSDGFISIVPSSSYQEASRSLLEEKCDKILAKCTHLLRESGIPFSAQKSIGA
                     PVDLILEHALVADLLVLGKRGEFARWDARALGATVEAVSRLVRKPLLAVGQEFSPIRD
                     ILVGYDGSDHANRALQYAAHLAEAAGGRVGVLCVSDDSELAGYYLGLASTYLAGYKVG
                     VNTLALTGRLDEILVRHAAAEGYGMIAIGAYGHSRIRETLLGSTTDHILRQAETPLLL
                     AK"
     gene            complement(3307..4320)
                     /locus_tag="PKM23_10925"
     CDS             complement(3307..4320)
                     /locus_tag="PKM23_10925"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNY92026.1"
                     /translation="MMRRICVLGAGGWGITLANYSRRLGHAVTLWEFDPAEAVRLDRE
                     RVRPAVLPGSEIDRSILITAELERALHGAEVILQVVPSHFARGVLRQIAPLGLSREVL
                     LVNCAKGIENDTQLRISEIVRQELPDFPDENYAVLSGPSHAEEVSRGIPSAVVIASSS
                     EEVAIALQEELNSRTLRCYRSHDVVGVELGGALKNVIAIAAGICDGAGFGDNTKAALQ
                     PRGLAEIARLGRKLGANLLTFAGLSGMGDLIVTCMSRHSRNRYVGEQIGRGQTLEEFL
                     SSMVMVAEGVRTSKSAVALARQCEVEMPICEQVYQALFEHKDPRAALEELMNREAKPE
                     LWH"
     gene            complement(4335..5342)
                     /gene="purM"
                     /locus_tag="PKM23_10930"
     CDS             complement(4335..5342)
                     /gene="purM"
                     /locus_tag="PKM23_10930"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNY92027.1"
                     /translation="MSITYQAAGVNLEAAEEATHQIAALAKSTFNSQVLREIGLFAGF
                     FALDLKKYSDPVIVSSIDGVGTKLKIAFATGRHESVGQDLVNHCVNDIMTCGADPLFF
                     LDYLGVGVLDPAAATALVGGMSNACRENGCALIGGETAEMPGFYAAGEYDLAGTIIGA
                     VNRDSIIDGGKIAAGDLLVGLPSTGLHTNGYSLARKVLFEMAKIDLAAHVEALGMTWA
                     EALLQIHRSYQKPIRLVRSHPALTGISHITGGGIEGNTRRLLRDGLNLAVEWGSWSVP
                     PLFRLIQEYGGIADEEMRRVFNLGIGMVLVARADGAEELLRLLEIEGGERAQVIGKIV
                     Q"
     gene            complement(5342..>5584)
                     /locus_tag="PKM23_10935"
     CDS             complement(5342..>5584)
                     /locus_tag="PKM23_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNY92028.1"
                     /translation="GRRRVNLDPGYSEAAKLVLATTKNFGHRIYLGQGIYGDVQLFWR
                     KGRFQSNPWTYPDYLEAKSLEFFSALRQDYLKEGKH"
BASE COUNT         1066 a         1641 c         1736 g         1141 t
ORIGIN      
        1 ggatcgacga taaaggccgg acggccgtac atggcagcca attcatgggc gaggatggcg
       61 ccgagattgg aggcgtgttc gccaaagtcg gccttctcaa gctgggtcag cattgccggc
      121 cccacccgga aggtcccact ggtgactgga tggagcaggc cgccacgacc gacaaaggcg
      181 tcgatttctt caatttttac gtttttttct tcaagtattt tcagaatagc ctgacgccga
      241 aacgcgaact gactgtagat ggtgggaaaa gatgccagct cctcgctgga gtggggcagg
      301 ctcgcggtaa agagcgggtt ttcgtcccgg aagagggcga tcttggtcga ggtcgaaccg
      361 ggattgatga ccaggatggt gaacacacag cctccgttat tcagattcga cgtggatgag
      421 ggattccaga cgcatcgggg cgaggcgttg gcgtgcgccg agaaagtcca gctcgatgac
      481 aaaggcgcag ccaatcactt cggctccgag tttgcgggca agccggaggg cggcctccac
      541 cgttccgccg gtggcgagca gatcgtcgac gatcacgaca cgctgaccgg gtttgagggc
      601 atcaacgtgc atctcgaggc tgtcgctgcc gtattcgagg gcatagctct ccgcgatggt
      661 cgtgaaaggc agcttgccgg gcttgcgcac cggtaccata ccgactccga gctcgtgagc
      721 gaggatggcg gcgaaaatga acccgcgcga ctcgataccg atcacggcgt cgacggtctc
      781 cccgtcaaag cgtttgcgca gctcggccat ggtctcgcgc agggcggggc catcaccgag
      841 cagggtggtt atatccttga agacaatccc cggtttggga aaatccggta tgctgcgaat
      901 tcgggcggcg agatccatgt catttccttt cacgattgag cggtgttgct gccgacgcac
      961 catccctccc actctggtgc agaatctgtt cccttgcggg aggatctggt tcaggggtag
     1021 atgcgcgaga tggttctcgg aaacgggatg gtttcgcgga tatgctgcag tccgcagatc
     1081 caggtgacgg tgcgttcgag ccccaatccg aatccggcat ggggcaccga accgaacttg
     1141 cgcagatcga gataccagtc ataattttcg gccgggagct ggtgctcatg aatgcggctg
     1201 agcagggtgt caagatcgtc ctcgcgctgt cctccgccga tgatttcacc gtagccctcg
     1261 ggggcgagga gatcaacaca gagggcgcga tcgggccgct caggatcgcg cttcatgtaa
     1321 aaggccttga cgccggcagg atagtgagtc accataaccg gtcggtcgta tctggcggag
     1381 atgatggtct catccggggc gccgagatcg tcaccgtaga cgaactggct gttctcctcc
     1441 ttgaggatct gagctgcatc ctcgtagtcc aggcgcggaa agggtttgcg gatgcactcg
     1501 agcttgctga ggtcgcgttc gagccgcttg agctcctccc gacgtttgtc gagcacgcgc
     1561 tcgacgatgg cgcagatgag cccttcggcc aattccatat ccccttcaag atcgcaaaag
     1621 gccacttcgg gctcgatcat ccaaaactcg gtaagatggc gccgggtctt ggatttttct
     1681 gctcggaagg tgggaccgaa gcagtagacc ttgccgaagg ccatggcggc ggcttccatg
     1741 taaagctggc cgctctgggt gagataggcc ttgtcctcaa aatagttggt ctcgaaaagg
     1801 gtgctggtcc cttcggcagc agcgggtgtg aagatggggg catcgatgag agtaaaattc
     1861 atattatcga aataatcgcg acatgcactg atgatctcgt gtcttacttt aagaacggcg
     1921 tgctgcaatg acgagcgcag ccagaggtgg cggtgctcca tgagaaaatc gatcccgtgt
     1981 tccttggggc tgatcggata ttcggcggcg agatggacca cttcgagagt ggagacctgc
     2041 agctcaaagc cgcccttggc gcgcttgtcc tcgctgacgg taccagtgat ccgaaccgaa
     2101 ctttcctgtg ttagtgccag tgtgctctcg aagagggcat cgccaacggt gtccctgaac
     2161 atcaccgcct ggatcaatcc ggtgccatcg cggagcatca gaaatatcag tttccccttc
     2221 gcggtcttgt tatagaccca cccctgcagg gttacctcct gcccctggaa tttggcgatt
     2281 tcatcgatat agactgtagg catgctcttg atttcctttc cgtgcaatag tgataatgat
     2341 cgatccggat ggattatttg gccaacagaa gcggggtttc cgcttgccgg aggatatggt
     2401 ctgttgtgct gccaagcagg gtttcacgaa tccttgagtg gccataggcc ccgatggcga
     2461 tcatgccgta accctctgct gcggcatgtc tgaccagaat ctcgtccagc ctaccggtga
     2521 gggcgagggt gttcaccccg accttgtagc cggccagata ggtggaggcc agcccgagat
     2581 aatagccggc gagttcgctg tcatcgctga cgcagagcac ccccaccctg ccgccggcgg
     2641 cctcggccag atgtgccgca tactgcaggg cacggttggc gtggtcggag ccgtcataac
     2701 ccacgaggat gtcccggatg ggtgaaaact cctgacccac tgccagcagg ggcttgcgca
     2761 ccagccggct caccgcttcc acagtggcgc cgagggccct ggcatcccag cgggcgaatt
     2821 cgccccgttt tcccaacacc agcaggtccg ccaccagggc atgctcgaga atgagatcca
     2881 ccggggcgcc gatacttttt tgcgccgaga aggggatccc actctcgcgc agaaggtgcg
     2941 tgcatttggc gagaatcttg tcgcactttt cctcgagcag gctgcgggag gcctcctggt
     3001 aggaactgga gggaacgatg gagataaagc cgtcactgcc gactgccgag gcccattcga
     3061 aaatgcgcac atcggcgact gtgagcaagt tcagacggct gccgaagcgc ccggcgagtt
     3121 cgatgccgtg cgagagcact gaatcggtat aggcggagcc gtcgacagcc agcaggatat
     3181 atttaaacat cttctcttcc ctcctctcga cccctcgtct tctgctccaa ctctggcgga
     3241 ggaagcgggg atcgcctggc agcgggcggg cgcatgagag cccaatcgcc cgctattttc
     3301 acgctctcag tgccagagtt caggtttcgc ctctcggttc atcaactcct ccagggcagc
     3361 gcggggatct ttgtgttcga agagggcctg atagacctgt tcgcagattg gcatctcgac
     3421 ctcgcactgg cgggcaaggg cgacggctga tttactggtc cggactccct ccgccaccat
     3481 aaccatggag gagaggaact cctcgagggt ttggccccgg ccgatttgct cgccgacata
     3541 gcggttgcgg ctgtggcggc tcatgcaggt gacgatcaga tcgcccattc ccgaaagccc
     3601 ggcgaaggtg agcaggttgg cccccagctt cctccccagt ctggcaattt cggccagtcc
     3661 gcgcggctga agggcggcct tggtgttgtc gccaaagccg gcgccatcac agatgccggc
     3721 ggcgatggcg atgacatttt tcagggctcc gcccagctcc actccgacga cgtcgtggct
     3781 gcgatagcag cgcagggtgc ggctgttcag ctcctcctgc agggcgatcg cgacctcttc
     3841 ggagctggag gcgatgacga ctgcggaggg gattccccgg ctgacctctt cggcatggct
     3901 tggccccgag agcactgcat agttctcgtc cggaaagtcc ggcagctcct ggcgcacgat
     3961 ctcggagatg cgcagctggg tgtcattttc aatgcccttg gcgcagttga ccagcagcac
     4021 ctcgcgcgag agtcccaaag gagcaatctg gcggagcaca ccgcgcgcga aatgggaagg
     4081 tacgacctgc aggatgacct cggccccgtg cagcgcgcgt tccagttcgg cggtgatcag
     4141 gatggagcgg tcaatttcac tgccggggag cacggcaggc cgcacgcgtt cgcgatccag
     4201 ccgtaccgcc tcggccggat cgaattccca tagtgtcacc gcatgaccca ggcggcggct
     4261 gtagttggcc agggttatgc cccatccccc tgctccgaga acgcagattc ttctcatgat
     4321 ttccttcctc tccgctactg gacgatcttg ccgatgacct gcgctctctc cccgccctcg
     4381 atttccagca gcctgaggag ttcctccgct ccatcggcgc gggcgaccag caccatgccg
     4441 atgccgagat tgaagacccg gcgcatctct tcgtcggcga tcccgccgta ctcctggatg
     4501 agcctgaaga ggggcggcac gctccagctt ccccactcca ccgcaaggtt gagcccatcg
     4561 cgcagcagcc ggcgggtgtt gccctcgatg cccccgccgg taatgtggct gattccggtc
     4621 agggcggggt ggctgcgcac caggcggata ggtttttgat agctgcgatg gatctgaagc
     4681 aaggcctccg cccaggtcat gccgagcgct tcaacgtgcg cggcgagatc gatctttgcc
     4741 atttcgaaaa gcaccttgcg ggccagcgag tagccgttgg tatgcaggcc ggtcgagggc
     4801 aggccgacga gcaggtctcc tgctgcaatc ttgccgccgt cgatgatgct gtcgcggttg
     4861 accgctccga tgatggtccc ggccaggtca tactctcctg cggcgtaaaa tcccggcatt
     4921 tcggcggttt cgcctcctat cagggcgcag ccgttctccc ggcaggcatt ggacatcccc
     4981 cccaccaggg cggttgctgc ggcgggatca agcacgccga cgccgaggta atcgaggaag
     5041 aacaggggat cagccccgca ggtcatgatg tcgttgacgc agtggttgac caggtcctgg
     5101 ccgaccgact catggcgtcc ggtggcgaag gcaatcttga gtttggtgcc gaccccatcg
     5161 atgctggaaa cgatgaccgg atcgctgtat tttttcagat cgagggcaaa aaagccggcg
     5221 aaaagcccga tctccctgag cacctggctg ttaaaggtcg atttggccag agccgcaatc
     5281 tgatgggtcg cctcttccgc cgcctcgagg ttgaccccgg ccgcctgata ggtgatggac
     5341 actagtgctt tccttccttg agataatcct gtcgcaacgc actgaaaaat tccagagatt
     5401 tggcttcgag atagtcggga taggtccatg gattggactg aaatcggccc ttgcgccaga
     5461 aaagctggac atcaccgtag atgccctggc cgagatagat gcggtggccg aaattcttgg
     5521 tggttgccag caccagcttg gccgcctcgc tatagcccgg atcgaggttg acgcgccggc
     5581 gccc
//
LOCUS       DAOPWU010000227         5566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14547_length_5566_cov_4.296861, whole genome shotgun sequence.
ACCESSION   DAOPWU010000227 DAOPWU010000000
VERSION     DAOPWU010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14547_length_5566_cov_4.296861"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="PKM23_10940"
     CDS             complement(<1..1440)
                     /locus_tag="PKM23_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92029.1"
                     /translation="MHKINHPKVRLLFCALGLLGVATESWAQKAPELETALIKVNRIW
                     TGVTANGGKGSFDYRAGFFPNDFDIIGVRGQEQDLWAGAGLKLATTNWRDPIDTLHTV
                     AIYGPTNDFMPNGKVTERISSYLRYKYTEQEIDFKPVALDLFGVHDPSRFGDLTCDQI
                     VQVTTENIFGVEVKRRIMGWSQTFNDDYLILDVEFTNTGSDTLTDFYISVESNGANTY
                     RSNGTRPSPGTGENFNTVTTWQHYYGGRTGDSLRVFYEYSADDPAVPGDLMGAPVTSQ
                     NGRLINPKFVWYSILHASEKPYTDPTGDVDDFLQPKVTYMGTATKFPYNSDGDDYGDK
                     NYWALRGAYSEFFPMSGSTWPGTLHAGNTDETGSPSYAAHPAGTIQANNSKMYCTFGP
                     YLFHPGEKLHLVFASGFSGLDIRTAKEVGAKWKNGTLENPPGIPDPRTGFFPENFAFP
                     VDASEVDMRKDRWVSTGIDSVMKSAWRAKW"
     gene            complement(1579..4845)
                     /locus_tag="PKM23_10945"
     CDS             complement(1579..4845)
                     /locus_tag="PKM23_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY92030.1"
                     /translation="MVFTRSWCRRGLDIVLILLLTLAGAQAQTRSRISGTVRDAASGE
                     SLPGVNVVIEESSLGAITNLKGEYVIINVPVGTVTVRASLMGYTAQKLTGVMVSADRV
                     ARADFALRQTVLQGSEVVVRAQRDELNLEVSNTQMVVSDAQLQDATGIREVNSFLTKL
                     PGVSTDNGYLTIRGGSADQTGMLVNGLSYVNSAAGNAETSIPLSAIEQISLMSGGYNA
                     EYGNFRSGLINVSTKSGSKEAYHGTLSLSMDNSHIRRFGGPFYDSKSQPLRVYLDPAV
                     AFTGTNEAWKDDAYLRQQHPTFTGWNTAAANFNKGNPKIPASPLDYYLLAAWMFMAVP
                     DYAGLEKAGIDISAITAEQRALFEEHRREEERSDWNIDGGFGGPVPLIGRYLGGATFY
                     ISNNSQSRHYVMPVVRKTQETYTTLATLRSQPRNNLTLTFNGLHKRQIGVSPIRPAFG
                     DFPDASRSGGFMPLNNIKNIRNVGDSYAYWFDPPFFPEIDQTTLMGGMTLNHVLSKSL
                     FYELSLSAMGIRNNSNVGDNRDQTILTQFGPFPVTEMPYGKFMYGTNKIKVDSTYTYT
                     YPSYDGLPGVPFRFRRKEGDLYDNTRVGQYRAKFDLAWQAGTRHYLKAGAEYNHIDIK
                     HNLWQKWNENYYNAYEFNYHRTPSQTGIYLQDQISLESMIANLGLRYDYYYGGGGKWP
                     TGDAFASEAFTPQVFGEDSVLFDYLESGRSWIWDSWEAYDREHPGFLQKIRNFHTLSP
                     RIGISFPVSDKSKFYFNYGHFRSNPPYYTMYLYRYRYTKNGLYDMSNPNLEPPRTISY
                     ELGVAYNFYDSFVAKISGYSKDVSGQHGEVTYTNAAGTINYDAWSNNEYQDIQGFEIN
                     LSKSDGGWINGWVNFNYMLKKSGLTGRETITDVTINNDQEGLYEGQESSSLPVPKINA
                     NLTLRAPDDLGPRLFGLHPLGGWNFTLFGEWERGDYFTFNPLSELHVNDNMRWPDYAM
                     IDLKVTKMFRVAGLQTTFFVDISNLFNFKVNYMSRGWPFDSAVNDEYYYLASLRLPMY
                     DSPDYDALRATYPGYFIAGKDKIGDLRSADKPYINDPNFNFWLYGQPRDIWFGLRFAF
                     "
     gene            complement(4901..>5566)
                     /locus_tag="PKM23_10950"
     CDS             complement(4901..>5566)
                     /locus_tag="PKM23_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92031.1"
                     /translation="RVLEYAGAILPKRDGVDTRIVLDVINGTASFEGTGYKKENSIPD
                     PSLICGIIDSQSQAGGWPELFSLPAPADSDHDGMPDFWENANGLNPADPEDRNLIDAE
                     GYTMLEVYLNSLTGATTEVRGEPAAQPAGLELKQNYPNPFNQGTAISFTLPEAGEVDM
                     SIHNAAGRVVRRLARGSLAAGNHRLQWDGRDEAGRPAASGLYFCRLRFHQQQKVIKLQ
                     LLR"
BASE COUNT         1032 a         1478 c         1861 g         1195 t
ORIGIN      
        1 ccacttggcg cgccaggcgc tcttcatcac cgagtcgatg ccggtgctga cccagcggtc
       61 cttgcgcata tccacctcgc tggcatcgac cggaaaggcg aaattctcag ggaaaaagcc
      121 tgttcgcgga tcggggatgc cgggaggatt ctccagggtg ccatttttcc attttgctcc
      181 cacctccttg gccgtgcgga tatcgaggcc ggaaaaaccg ctggcgaaga cgaggtggag
      241 tttttcgccg ggatggaaga ggtaaggccc gaaggtgcag tacatcttgg aattgttggc
      301 ctggatcgtc ccggcaggat gggcggcgta gcttggactg ccggtctcgt cggtgttgcc
      361 ggcgtgcagg gtgccgggcc aggtgctgcc gctcatgggg aagaattcac tgtaagcccc
      421 gcgcagggcc caataattct tgtcgccgta atcgtcccca tcactgttgt aggggaactt
      481 ggttgccgtg cccatgtagg tcactttggg ctggagaaaa tcgtccacat cgccggtcgg
      541 gtcggtatag ggcttttcag aggcgtgcaa aatcgaatac cagacgaact tgggattgat
      601 gaggcggccg ttctgtgaag tgaccggcgc gcccatgagg tcgccgggga ccgccggatc
      661 gtcagcgctg tattcataga atacgcgcag gctgtcgccg gtgcggccgc cgtaatagtg
      721 ctgccaggtg gtgacggtgt tgaaattctc ccctgtgccg ggggagggcc gggtgccgtt
      781 ggagcggtag gtgttggcgc cgttggattc gacgctgata tagaaatcgg tgagggtatc
      841 actgccggtg ttggtgaatt cgacgtcgag gatgagatag tcgtcattga aggtctggct
      901 ccagcccatg atgcggcgtt tgacctcgac cccgaaaatg ttctcggtgg taacctggac
      961 gatctggtcg caggtgaggt cgccgaatct ggagggatcg tggacgccga agaggtcgag
     1021 ggcgaccggc ttgaaatcga tctcctgctc ggtgtatttg taacgcagat agctgctgat
     1081 gcgctcggtg accttgccgt tgggcatgaa atcgttggtc ggtccgtaga tggcgacagt
     1141 gtggagggta tcgatgggat cgcgccagtt ggtggtggcg agtttcagac cggctcccgc
     1201 ccacaggtcc tgctcctggc cgcgcacgcc gatgatatcg aaatcattgg ggaaaaagcc
     1261 ggcacggtag tcaaagctgc ccttgccgcc gttggcagtg acgccggtcc agatgcggtt
     1321 gaccttgatg agggcggttt ccagctccgg ggccttctgg gcccaggact ccgttgcgac
     1381 gccgagcaag ccgagtgcgc agaacagcag ccgtaccttg ggatgattta ttttatgcat
     1441 gcgacgctcc aatttattcg caggattaca caaaacaaaa tttctcacag tggcacggag
     1501 acgcagcaga gggcgcaggg atctacaggt ttttctcttt ttctcggcgt ctctgtgccc
     1561 ccgtgagaaa attatcgctt agaacgcaaa gcgcaggccg aaccagatat cccgcggctg
     1621 gccgtaaagc cagaaattga aattgggatc gttgatatag ggcttgtccg ccgagcgcag
     1681 gtcgccgatc ttgtccttgc cggcgatgaa atagccggga taggtggcgc gcagggcgtc
     1741 atagtccggc gaatcgtaca tcggcagccg cagcgaggcc aggtaataat attcgtcgtt
     1801 gaccgcgctg tcgaagggcc agccccggct catgtaattg accttgaaat tgaaaaggtt
     1861 gctgatgtcg acgaaaaagg tggtttggag accggcgacg cgaaacattt tggtcacctt
     1921 gaggtcgatc atggcataat ccggccagcg catgttgtcg ttgacgtgga gttcactgag
     1981 gggattgaag gtaaaataat cgccccgctc ccactcgcca aagagggtga aattccagcc
     2041 gcccagggga tgcaggccga acagccgcgg ccccagatca tccggggcgc gcaaggtgag
     2101 gttggcgttg atcttgggca ccggcaggga gctggactcc tggccttcgt agagcccctc
     2161 ctggtcgttg ttgatggtga catcggtgat ggtctcgcgg ccggtcagtc ccgacttctt
     2221 gagcatataa ttaaaattga cccagccgtt gatccagccg ccgtcgctct tgctgaggtt
     2281 gatctcgaag ccctggatgt cttggtattc gttgttggac caggcgtcgt agttgatggt
     2341 gccggcggca ttggtatagg tcacctcgcc gtgctggccg ctgacatcct tggagtagcc
     2401 cgagattttg gcgacaaagc tgtcataaaa attataagcg acgccgagct cgtaggagat
     2461 ggtccgcggc ggctcgaggt tgggattgga catgtcgtag agaccgttct tggtatagcg
     2521 ataacggtag agatacatgg tgtagtaggg cggattggag cggaagtggc catagttgaa
     2581 ataaaacttg gacttgtccg agaccggaaa ggaaatgccg atccggggac tgagggtatg
     2641 aaaattcctg atcttttgca ggaagccggg atgctcgcgg tcgtaggcct cccaggagtc
     2701 ccagatccag gagcggccgg actccagata gtcgaagagg accgaatcct cgccgaagac
     2761 ctggggggtg aaggcttcgc tggcaaaggc gtccccggtc ggccatttgc cgcctccgcc
     2821 gtaatagtaa tcatagcgaa ggccaaggtt ggcgatcatc gattccaggc tgatctgatc
     2881 ctggaggtag atgccggtct ggctcggggt gcggtgatag ttgaactcgt aggcgttgta
     2941 gtaattctcg ttccactttt gccagaggtt gtgtttgata tcgatgtggt tgtactccgc
     3001 gcccgccttg agatagtggc gggtgccggc ctgccaggcg aggtcgaatt tggcgcggta
     3061 ctggccgacg cgggtgttgt cgtagagatc gccctccttg cggcggaagc ggaagggcac
     3121 acccggcagg ccgtcgtagc tgggataggt ataagtgtag gtgctgtcca ccttgatctt
     3181 gttggtgccg tacatgaact tgccgtaggg catctcggtg accggaaagg ggccgaactg
     3241 ggtgaggatg gtctggtcgc ggttgtcgcc cacattggag ttgttcctga tgcccatggc
     3301 gctgaggctg agttcgtaaa aaaggctctt gctcagcaca tggttgaggg tcattccgcc
     3361 catcagggtg gtctggtcga tctcggggaa gaagggggga tcgaaccagt aggcatagct
     3421 gtcgccgaca ttcctgatgt ttttgatatt attcaggggc atgaatccgc cggagcggct
     3481 ggcgtcggga aaatctccaa aggccggccg gatcgggctc accccgatct ggcgcttgtg
     3541 cagaccattg aaggtgaggg tgagattatt gcgcggctgg ctgcgcagcg tggccagagt
     3601 ggtataggtc tcctgggtct tgcgcaccac cggcatgaca tagtggcggg actgggaatt
     3661 gttggagata taaaaagtgg ccccgccgag atatcggccg atcaggggca ccgggccgcc
     3721 gaagccgccg tcgatgttcc agtcgctcct ctcctcctcc ctacggtgct cctcgaagag
     3781 ggcgcgctgt tcggcggtga tggcgctgat gtcgatgccc gccttttcga gccctgcgta
     3841 atcggggacg gccataaaca tccacgccgc cagcaggtaa taatccaggg ggctagccgg
     3901 gattttcgga ttgcccttgt tgaaattggc cgctgcggta ttccagccgg tgaaagtcgg
     3961 atgctgctga cgcaaatagg cgtcatcctt ccaggcctca ttggtaccgg taaaggcgac
     4021 ggcgggatcg aggtagacgc gcaggggctg gctcttgctg tcgtagaacg gcccgccgaa
     4081 gcggcggata tgggagttgt ccatcgacag gctgagggtg ccgtgatagg cctcctttga
     4141 gccggacttg gtgctgacgt tgatgaggcc ggagcggaaa ttgccgtact cagcgttgta
     4201 gccgcccgac atcagcgaaa tctgctcgat ggcgctgagg gggatgctgg tctcggcatt
     4261 gccggccgcg ctgttgacat aggagaggcc gttgaccagc atgccggtct ggtcggccga
     4321 accgccgcgg atggtcagat agccgttatc ggtcgatacc ccgggcagtt tggtgagaaa
     4381 ggagttgacc tcgcggatgc cggtcgcgtc ctggagctgg gcgtcgctca ccaccatctg
     4441 ggtgttggag acctcgaggt tgagctcgtc gcgctgtgcg cgcacaacca cctcgctgcc
     4501 ttgcagcacg gtttggcgca gggcaaaatc ggcccgggcg acgcggtcgg cggagaccat
     4561 gaccccggtt agtttctgcg ccgtgtagcc catcagcgag gcccgcacgg tgacggtgcc
     4621 gaccgggacg ttgatgatga cgtattcgcc cttgaggttg gtgatggcgc cgaggctact
     4681 ctcctcgatg acgacattga ctccgggcag gctctccccg ctcgcggcat ccctgaccgt
     4741 tccgctgatg cggctgcgcg tctgcgcctg tgcgccggcg agggtcagca ggaggatgag
     4801 cacaatatcc agccccctcc tgcaccacga tcttgtgaat accatagcta ctccttctct
     4861 agcgctcatg tgcgagtggt cagctcctgg gcagccctgc tcagcgcaga agctgcagct
     4921 tgatcacctt ttgctgctga tggaagcgga ggcggcagaa atagagtccc gaggccgcag
     4981 gcctgcctgc ctcatcgcgg ccgtcccact gcaggcggtg gttcccggcg gccagggagc
     5041 cgcgcgccag tcggcgcacg acgcggccgg ccgcattgtg gatcgacata tccacctcgc
     5101 ccgcttccgg cagggtgaag gaaatggccg tcccctggtt gaagggattg ggataattct
     5161 gcttgagctc aaggccggcc ggctgcgcgg cgggttctcc ccgcacctca gtggtcgccc
     5221 cggtcaggct gttgagatag acctcaagca tggtatagcc ttcggcatcg atgaggttgc
     5281 ggtcctcggg atctgcggga ttgaggccgt tggcgttttc ccaaaagtca ggcatgccgt
     5341 cgtggtcgct gtcggccggg gcgggcaggc tgaaaagctc gggccagccg ccggcctggc
     5401 tctgcgagtc gatgatgccg cagatcaggg agggatcggg gatgctgttc tccttcttgt
     5461 agcccgtccc ctcaaaggaa gcggtgccgt tgatcacatc cagaacgatg cgggtgtcga
     5521 caccatccct tttgggcagg atcgccccgg cgtattcgag cacgcg
//
LOCUS       DAOPWU010000228         5561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14570_length_5561_cov_5.518344, whole genome shotgun sequence.
ACCESSION   DAOPWU010000228 DAOPWU010000000
VERSION     DAOPWU010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14570_length_5561_cov_5.518344"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKM23_10955"
     CDS             <1..247
                     /locus_tag="PKM23_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92032.1"
                     /translation="FAAEGSGELLLVAARLEGEGVLFHLREVEGRPATLSLNPAFSSG
                     RIVLCDPLGRSLEEVESISFAPFEVRFVRVITSLKRS"
     gene            249..2999
                     /locus_tag="PKM23_10960"
     CDS             249..2999
                     /locus_tag="PKM23_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNY92033.1"
                     /translation="MASSARRRLTLAAILSLSALLPAVQAAAPAGGTLPYKDPDLAVE
                     VRVRDLLGRMTLEEKLWQLFMAPGDLEHDRHKLDKGIFGLQFHTTPAGSAAAAQILRY
                     GPSGSAAAMAEMINSAQRFFVEETRLGIPIIPFDEALHGLVRDGATAFPQAIGLAATW
                     DPELIGSVAAAIARECRSRGIRQVLSPVVNVATDVRWGRVEESYGEDPLLSAEMGLAF
                     VRAFEASGIITTPKHFVANHGEGGRDSYPVHHSERLLEELYLPPFAACIGRGGSRSLM
                     ISYNSLDGRPCTANDWLLNRKLKGEWGFRGFVISDAGAVGGITDLHGVSADYEASVKS
                     AFENGLDVLFQSTIDHFELFFTRALRGSYIDSLVIDRAVARVLRAKFELGLFEDPYVD
                     PAEAGRVNGTTRHRELARQAARESIVLLKNRAQTLPLSRAIRSISVIGPEAAEARLGG
                     YSGPGIRPVSILEGIRARAGELIKVNYARGCSREETEVTVIPERWLRRSPSPEKNEAA
                     WRGAPRSPSLEAGGGEAGLVGRYWANPDFDGAPLFERFDARIDFNWSLFSPHPGLQRE
                     WFSAAWSGELVAPADGRLRIGLEGNDGFRLWIGDSLLIDNGEKGSYRRVMAPWPVRKN
                     LAYPLRIEFCERKGGGRLQLLWDYRERDEAGDGMEEAVAAARASEVAVICAGIVEGEG
                     LDRASLRLPGRQEELIRAVAATGVPVIVLLTAGSAVTMNSWLDEVEAVAALWYPGEEG
                     GHAAAEMLFGDYNPAGRLPVTFPLAEGQLPLVYHHKPTGRLDYYHDLPGEPLFPFGYG
                     LSYTRFSYKGLELASPAQDSLLVRFELRNSGPRGGDEVVQLYTRRPRSDLARPLMALK
                     KFARIHLEPGESRQVVLTLGRGELEELDQALRPVVEPGRIEIMIGASSADIRLRGGTE
                     LP"
     gene            complement(3074..3199)
                     /locus_tag="PKM23_10965"
     CDS             complement(3074..3199)
                     /locus_tag="PKM23_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92034.1"
                     /translation="MSTSAWIMMLTTWAIVAYFCIHFFIKVARTPQGKKHSVKDA"
     gene            complement(3196..4833)
                     /locus_tag="PKM23_10970"
     CDS             complement(3196..4833)
                     /locus_tag="PKM23_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNY92035.1"
                     /translation="MVQTNERWGSRVGLILAMAGNAVGLGNFLRFPAQAVQNGGGAFI
                     IPYLVSFLLMGIPLLWIEWAVGRHGGQFGHHSAPGMMERIGRGRAWKYVGVFGIFTNL
                     AVAAYYAYIESWTLGYVWYSLTGAFAGQSAEQVSHFFSAYLNIGSGHFLNISGPAILF
                     FLITISINIYILSRGLAKGVEIASKIGMPLLLVFGAFLAIRALTLNAGEAGAVNNALD
                     GLNFLWQPQFDSLTNPKVWLAAAGQIFFTLSVGMGTIQCYAAYLREKDDIALNAATAG
                     WMNEFVEVILGGSIIIPIAVAYLGLPWIQANVGFEMGFRTMPTLFQNWGPFLAAAAGV
                     AWFGLLFFAGITSSLAMGQPVMAFLQDEFNLSRKRSALTLGGILLMLSAPCLLLYEMG
                     AFGEYDYWAGTFSLVAFALLESIAFAWVFGIDKGWEEINRGADIKVPHFFKFVIKYVT
                     PLFLLAVFVGSLLKPEAGNWAAAFGSLFSGGGWPFAADSVIGTVLNVGIAEKRWFIDG
                     MPTQIFILNMTRLLLLSTFIIIGVAVYAAWRRKGAKA"
     gene            5083..>5561
                     /locus_tag="PKM23_10975"
     CDS             5083..>5561
                     /locus_tag="PKM23_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92036.1"
                     /translation="MKSSRDSVGLPLSVPPKSVADGRSVVPDELSFWPYFQPGRAAGE
                     QIFSFDFGKDIFVHINAPVPLASSKPLLLILYALPNGNTLDQTFGKRLEPGDDWHYDI
                     QHVGAQIRFLRALDPPFTPVLVLLQTRQRSWPAWNTAHPADHGMQIVNLVNSLRQIFR
                     "
BASE COUNT         1000 a         1744 c         1813 g         1004 t
ORIGIN      
        1 ctttgccgcc gaaggtagcg gtgagctgct cctggtcgcc gcccggctgg agggggaggg
       61 ggtgcttttc catttgcgtg aggttgaggg gcggccggcg accctgagcc tcaacccggc
      121 tttcagcagc gggcggatcg tgctctgcga tcccctcggc cggtccctcg aagaggtgga
      181 gagcatcagc tttgcgccct ttgaggtgcg ctttgtgcgc gtaattacat ccttgaaaag
      241 gagttgagat ggcttcatct gcacggcgcc ggctgaccct ggccgctatt ctgtccctct
      301 ccgcgctgct tccggccgtg caggcggccg cgcctgccgg gggaactctc ccctacaagg
      361 atccggatct ggctgtggag gtgcgcgtcc gggatctgct cggccgcatg accctggagg
      421 agaagctctg gcagctcttc atggctcccg gcgacctcga acacgaccgg cacaagctgg
      481 acaagggcat tttcgggctg cagtttcaca ccactccggc cgggagcgcc gccgctgcgc
      541 agattctccg ctacggcccc tcgggatcgg cggcggccat ggcggagatg atcaattcgg
      601 cccagcgctt tttcgtggag gagacccgtc tcggcatccc gatcatcccc ttcgacgagg
      661 ccctgcacgg cctggtgcgc gacggcgcca ccgcctttcc ccaggcgatc ggactggccg
      721 ccacctggga tccggagctg atcgggagcg tcgccgccgc cattgcccgg gagtgccgct
      781 cccgcggtat ccgccaggtg ctgtcgccgg tggtcaatgt cgccaccgac gtgcgctggg
      841 gccgcgtcga ggagagctac ggcgaggatc ccctgctctc tgcagaaatg ggcctcgcct
      901 ttgtgcgcgc gttcgaagcc tccggcatca tcaccacccc caagcatttc gtcgccaacc
      961 acggcgaagg cgggcgcgac agctatccgg tccatcacag cgagcggctg ctcgaggaac
     1021 tctatctgcc gccctttgcc gcctgcatcg gccggggcgg ctcgcgctcc ctgatgatct
     1081 cctacaacag cctcgatggc cgtccctgca cggccaatga ctggctgctc aaccgcaaac
     1141 tcaaggggga gtggggtttc cgcggatttg tcatctctga cgccggcgcc gtcggcggca
     1201 tcactgacct gcacggagtc agcgccgatt atgaggcctc agtcaagtcg gcctttgaaa
     1261 acggcctcga cgtcctcttc cagtcgacca tcgaccattt tgagctcttt ttcacccggg
     1321 ctctgcgcgg cagctatatc gacagcctgg tcatcgaccg ggcggtggcc cgcgtgctgc
     1381 gcgccaaatt cgagctgggg ctctttgagg atccctatgt cgatccggcc gaggccgggc
     1441 gcgtcaacgg gaccacccgc caccgggagc tggcccgcca ggcggcgagg gagagcatcg
     1501 tgctcctcaa aaaccgggcg caaaccctgc cgctctcgcg cgcgatcaga tccatttcgg
     1561 tcatcggccc ggaggcggcg gaggcccggc ttggcggcta cagcggtccg ggaatccggc
     1621 cggtctccat tctggagggg atccgcgccc gcgccgggga actcatcaag gtgaattatg
     1681 cccggggctg cagccgtgaa gagacggagg tcacggtcat cccggaacgc tggctgcgcc
     1741 gctccccatc gccggaaaaa aacgaggccg catggcgcgg agccccgcgc tcgccgtctc
     1801 tcgaggccgg cgggggggag gccggcctgg tcggccgtta ctgggccaat cccgatttcg
     1861 acggcgcacc tctattcgag cgtttcgacg cccgcattga tttcaactgg agcctctttt
     1921 ccccccatcc cgggctgcag cgggagtggt tcagtgccgc ctggtcgggg gagctggtgg
     1981 ccccggctga cggccggctg cgcatcggcc tggagggcaa tgacggcttc cggctctgga
     2041 tcggcgactc gctgctgatc gacaacgggg agaagggctc ctaccgccgg gtcatggctc
     2101 cctggccggt gcgcaagaac ctcgcctatc ctctccggat tgagttctgc gagcgcaagg
     2161 ggggcggacg gctccagctg ctgtgggatt accgggagag ggatgaagcg ggcgatggca
     2221 tggaggaggc ggtggccgct gcgcgcgcaa gcgaggtggc ggtgatctgc gccggcatcg
     2281 tcgaggggga ggggctcgac cgcgcctccc tgcgcctgcc cggccgccag gaagagctca
     2341 tccgcgcagt ggccgccacc ggcgtgccgg tcatcgtgct gctcacagcc ggcagcgccg
     2401 tgaccatgaa ctcctggctc gatgaggtgg aagcggtggc ggccctctgg tatcccggcg
     2461 aggagggggg ccatgccgcc gccgagatgc tcttcggcga ttacaatccc gccggccgcc
     2521 tgcccgtcac ttttcccctg gcagaggggc agctgcccct ggtctaccat cacaagccca
     2581 ccggacggct ggactattac catgacctgc ccggcgagcc cctcttcccc ttcggctatg
     2641 gactgagcta tacccgcttc tcctacaagg gtctggaact cgccagcccg gcgcaggact
     2701 cgctgctggt ccgcttcgag ctgcgcaaca gcggtccccg cggcggcgac gaggtggtcc
     2761 agctttatac acgacggccg cgctcagatt tggcgcggcc gctcatggcg cttaaaaagt
     2821 ttgccaggat tcatctcgag ccgggcgaga gccggcaggt agtactgacc ctgggcaggg
     2881 gcgaactgga ggaacttgat caggccttgc ggccggtggt cgagccgggc cggatcgaga
     2941 tcatgatcgg cgcctcatcg gccgacatcc ggttgcgcgg cgggacggag ctgccctgag
     3001 gcccttgccg gcgcgggata tggatgtctc tccacctgga gcacccgtcc gctggccggc
     3061 gttcgtggcc cgctcaggcg tccttgacgg agtgcttttt cccctgcggg gtgcgcgcga
     3121 ccttgatgaa aaagtggatg cagaaatagg ccacaatggc ccaggtggtc agcatcatga
     3181 tccaggcgct cgtgctcatg ctttcgcccc tttcctgcgc caggcagcgt aaacggccac
     3241 gccgatgatg atgaaggtgg acagcagcag cagccgggtc atgttgagta tgaagatctg
     3301 cgtcggcatg ccgtcaatga accagcgctt ctcggcgatg ccgacattga ggacggtgcc
     3361 gatgacactg tcggcggcga agggccagcc cccgccggaa aagaggctgc caaaggcggc
     3421 cgcccaattg cccgcctcag gcttgagcag tgaaccgaca aagaccgcga gcagaaagag
     3481 cggcgtcaca tatttgatca caaatttgaa aaagtggggc accttgatgt ccgctccccg
     3541 gttgatctcc tcccagccct tgtcgattcc gaagacccag gcgaaggcga tcgattcgag
     3601 gagggcgaag gcgaccaggg agaaggtgcc ggcccagtaa tcgtactccc cgaaagcccc
     3661 catctcatag aggaggaggc agggggccga gagcatgagc aggatgccgc caagggtcag
     3721 ggcggagcgc ttgcggctga ggttgaactc gtcctggaga aaggccatca ccggctggcc
     3781 catggccagg gaactggtga tgcccgcgaa gaagaggagg ccgaaccagg cgacaccagc
     3841 cgcagcggcg aggaagggcc cccagttctg gaagagggtc ggcatggtgc ggaagcccat
     3901 ctcaaagccg acgttggcct ggatccaggg gaggccgagg taggcgaccg cgatcgggat
     3961 gatgatcgag ccgccgagga tgacttcgac gaattcgttc atccagccgg ccgtggcggc
     4021 gttgagggcg atgtcgtcct tctcgcgcag gtaggcggca tagcactgga tggttcccat
     4081 cccgaccgag agggtgaaaa agatctgtcc tgctgccgcc agccagacct tgggattggt
     4141 aagggagtcg aactgcggct gccagagaaa gttgagccca tcgagggcgt tattgaccgc
     4201 cccggcctcc ccggcattga gggtgagggc gcggatagcc agaaaggcgc cgaagacgag
     4261 cagcaggggc ataccgattt tactggcgat ttccacccct ttggcgaggc cgcgcgagag
     4321 aatgtagatg ttgatcgaga tcgtgatcag aaaaaaaagg atggcggggc cggatatatt
     4381 gagaaaatgg ccgctgccaa tgttgagata ggcgctgaaa aagtgcgaaa cctgctccgc
     4441 gctctgtccg gcaaaggcgc cggtgaggga gtaccagaca tagcccaggg tccaggattc
     4501 gatataggca tagtaggcag ccactgcgag gttggtgaag atgccgaaga ccccgacgta
     4561 cttccacgcc cggcccctgc cgatgcgttc catcatcccc ggggctgaat gatggccgaa
     4621 ctggccgccg tggcgcccaa ccgcccattc aatccacagg agcggaatcc ccatgagcag
     4681 gaacgagacc aggtagggaa tgataaaggc gccgccgccg ttttgcacag cctgggcggg
     4741 aaagcgcaga aaattgccca gaccgaccgc attgccggcc atggccagaa tcaggcctac
     4801 ccgcgatccc catctctcat tggtttggac catccagtct cctttgtgcg gtttgtatgc
     4861 cggtgtgtga attatcggca attttaccca aatccggtcc taaaagcaag aaatttttta
     4921 gtatatttta tcagccaagc cggcgggatg gttccaggag tgttgccggc cggtgggaag
     4981 catgataaaa aaatataagc gaatattatg cacttaaata gaacggtcct gcttgccctg
     5041 cttctgaccg gccttgtcgc gagccgggcg ggagcaggaa aggtgaaatc ctctcgggac
     5101 tctgtcggcc tcccgctctc tgtgccgccc aaatccgtcg ctgacgggag gagcgttgtt
     5161 ccggacgaac tatccttttg gccttatttt caacccggcc gcgctgctgg cgagcagatc
     5221 ttcagctttg actttgggaa ggacattttt gtccacatca acgccccggt accgctggcc
     5281 tccagcaaac cgctgctgct catcctttat gccctgccga acggcaacac cctcgaccag
     5341 accttcggca agcggctcga acctggcgac gactggcact atgatattca gcatgtcggc
     5401 gctcagatcc gttttctgcg cgccctggat ccgcccttta cccccgtgct agtgctgctg
     5461 cagacccggc agcgcagctg gccggcgtgg aatacggcgc atcccgccga tcacgggatg
     5521 cagattgtca acctggtcaa cagcctgcgg cagattttcc g
//
LOCUS       DAOPWU010000229         5486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14848_length_5486_cov_4.159823, whole genome shotgun sequence.
ACCESSION   DAOPWU010000229 DAOPWU010000000
VERSION     DAOPWU010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14848_length_5486_cov_4.159823"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1604)
                     /locus_tag="PKM23_10980"
     CDS             complement(63..1604)
                     /locus_tag="PKM23_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNY92037.1"
                     /translation="MQRYTMFFAMALLVTALPAAAAPGPEAAPVRVDEPLSEGTMSDQ
                     AGDQFTLFLQRVYSLPEGARQAVADSFYQAAGNLPLIVADSAVYYLYRGGATSVALAG
                     DANGWDPSRHSFNRVSGTNLWFLRQTYEPDARLDYKFVLNNSSWILDSRNPLLCYGGY
                     GPNSELRMPEYPPAAECMTDTSVSGGTLEKGSFVSAILGNSRFLRVYLPADYDPERED
                     GYPLVLFHDGLEFLALGDVKTVLDNLIGWKRIEPMIGVFVPPVSAGQRIEEYSGATAV
                     HFERFIIEELMPHIDATYNTSRDPSKRAMMGPSLAALISAQICFHHPEVFGLSGLYSP
                     AMWPNERAVLMQLLASARPFQKCYIDAGSYEPSLIGDAETLFSHLLDNGVEARFRLWH
                     EGHSWGSWRAHVDEALEFFFPLSTGIERIGEVGTPAFQLHPAYPNPFNAGVTLEFSLP
                     QPARVALEIFNVRGELVEQLLQGGLQAGRYRHLWMAAGRPSGLYIYRLRVGADVHTGR
                     VLLLR"
     gene            1915..3198
                     /locus_tag="PKM23_10985"
     CDS             1915..3198
                     /locus_tag="PKM23_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY92038.1"
                     /translation="MKRLLLMPVLALLILLAALQEPATAQYDPERLYQHLQSVYDRND
                     GDVSGYLIDELTQFGLIFPDDARAADASWLLAKIYLDKNKRHEALAAAFKTLYLYPRS
                     LHEGECRDIIQNVLAREVVYAPKKGLLESAVYGTAVDAATLERWHAYLNLLKDLDQPR
                     LYDWSLTEARTFVTRFPGDSLIYPVLEWTGDLYQRLGKIWEAIYSYRKLEEIFPDHPQ
                     LPYALHQQALLLSERAAKHEDAVSRLTRILAHYPESDFAPTAAYTIGKIKERELKDYN
                     GAIEAYRKLVAGWPKDERAVEVLLAIADIQDRRLKQYDLALATYDEIIKDYVSDPRGA
                     RALELTGDLYAENLKDYLNAASSYARVSKLYTADEKAPDLLIKAGDLCAERLKDYRKA
                     IEYYEMVANLYPASKKVKEANKRIDKASKKITDNL"
     gene            3304..3774
                     /locus_tag="PKM23_10990"
     CDS             3304..3774
                     /locus_tag="PKM23_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HNY92039.1"
                     /translation="MKQRTDRHHAIRAMIESRPIASQNQLLEELQALGFDVTQATLSR
                     DLLLLGAMRIPDPEKGYIYALPGAAALAPPLRDTSALHACRSLAFSGHLAVVKSLPSF
                     APSIGLLLDGLNLPEVVGTVAGDDTLIVVLAEGTSHHRFRQILLERLPELRDRF"
     gene            3881..5131
                     /locus_tag="PKM23_10995"
     CDS             3881..5131
                     /locus_tag="PKM23_10995"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNY92040.1"
                     /translation="MKEKVILAYSGGLDTSVILKWLELKGYDVIAYVADVGQKEDFEA
                     VREKALKTGASKVYIEDLKEELVQEYIFAAARGNALYEGRYFLGTAIARPLIAKRQIE
                     IAAREGAHYVSHGATGKGNDQVRFELAYYALQPDIQVISPWKDAEFLAQFKGRSDMIA
                     FAEKWSIPVKASIAKSYSEDENLMHISHEAGILEDPLFRPHEEIFSRTVAPQQAPDAE
                     TLLEIEFADGYPVKVTNRSDSTVVTGALPLFTYLNRVGAANGVGRVDMVENRFVGIKS
                     RGVYETPGATILWAAHRDLEGIAMDKEVMRIRDMLSPLFSELIYNGFWFSPEMDFLLS
                     AFEKSQEKINGSVLLSIYKGNVTPIGRQSPISLYNQDLSSMDVEGGYNAMDAGGFIRI
                     HAIRLKAHNIVLKNGKPYTWRHKE"
     gene            5133..>5486
                     /locus_tag="PKM23_11000"
     CDS             5133..>5486
                     /locus_tag="PKM23_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNY92041.1"
                     /translation="MKLWQNPPAAAAPPDPLTERIEQFTAGADPILDLELLPWDGLAS
                     KAHARMLAKVGLLSQEEAAALIAGLDGILALHRRGEFTISRADEDGHTAIENHLVQRC
                     GEAGLKIHTGRSRNDQ"
BASE COUNT         1178 a         1816 c         1499 g          993 t
ORIGIN      
        1 gcaccacctt ctcctttaaa ccgatcatag tcctgtcctt gccattggtc cattgtttca
       61 gttcagcgca gcagcagcac ccggccggtg tgcacgtccg cccccacccg gagccggtaa
      121 atataaagac cgctcgggcg cccggccgcc atccagaggt gacggtaacg cccggcctgc
      181 agccccccct gcagcagttg ctcgacaagc tccccgcgca cattgaaaat ttccagggca
      241 acccgggccg gctgcggcag gctgaattcg agggtcactc cggcgttgaa gggattggga
      301 taggccgggt ggagctggaa ggccggcgtc ccgacctcgc cgattcgttc gatccccgtc
      361 gagagcggaa aaaagaattc gagggcctcg tcgacatggg cgcgccagct gccccagctg
      421 tggccctcat gccagaggcg gaacctggcc tcgacgccgt tgtcgaggag atgggagaaa
      481 agggtctccg catccccgat cagcgaaggt tcgtagctgc cggcatcgat gtagcacttt
      541 tgaaagggac gggcggaggc gagcagctgc atgagcaccg ccctctcatt cggccacatc
      601 gccggtgaat agaggccgct gaggccgaaa acctccggat gatgaaagca gatctgggcc
      661 gagatcaggg cggccagtga aggacccatc atcgcccgct tggaaggatc tcgggaggtg
      721 ttgtaggtcg catcgatatg gggcatgagc tcctcgatga tgaagcgctc aaaatggacc
      781 gccgtggccc cgctgtactc ctcgatccgc tgcccagccg ataccggggg cacaaaaacg
      841 ccaatcatcg gttcaatccg cttccagccg atgaggttgt cgagcacggt cttgacatcc
      901 cccagggcaa ggaactcgag gccgtcatga aataggacca ggggatagcc atcctcccgc
      961 tccggatcat aatccgcagg gaggtagaca cgcagaaagc ggctgttgcc gagtatggcg
     1021 ctgacaaaac tccccttttc gagggtcccg ccgctcacgc tggtatcggt catacactcc
     1081 gccgccggcg gatattcggg catacgcagc tccgaattgg ggccatagcc gccgtagcag
     1141 agcagggggt tgcgcgaatc gagaatccag ctcgagttgt tgaggacaaa cttgtagtcg
     1201 agccgggcat cgggctcgta ggtttgacgg agaaaccaga gatttgtccc ggaaacccgg
     1261 ttgaatgagt gacggctcgg atcccagccg ttggcgtcgc cggcgagggc cactgaggtg
     1321 gccccgccgc gatagagata atagacggcc gaatcggcca caatgagagg cagattcccg
     1381 gctgcctgat aaaagctgtc ggcgacagcc tggcgcgccc cctccgggag ggagtaaacc
     1441 cgctgcaaaa ataaggtgaa ctggtcgccc gcctgatcgc tcattgtacc ttcgctgaga
     1501 ggttcatcca ctcggaccgg cgccgcctca ggcccggggg ccgcggcggc cggcagtgcc
     1561 gtgacaagga gcgccatcgc gaaaaacatc gtgtatcgct gcataggctt ttcctcaaca
     1621 aaatgtagat cctgaatagt atacccaata tttgcttctt gaacaagcaa aatgatgatt
     1681 gaaattcata acatcggccg gtctgccagc gacggaaacg atcaatgtta tcgctggata
     1741 tccgcccatt ccagcgtcgg atagccccgg ccagacagaa gattttttca ttgtttagtt
     1801 gtatattttt taatatactt tatgagattc atgcagccga agcatcctca ggctgtatga
     1861 aagccttcgc actgcatccc tggcaggcat tgacctaaca cgaggaacca gcccatgaaa
     1921 cgactccttc ttatgccggt tctggcactc ctgatcctgc tcgctgccct tcaggagccg
     1981 gccacagccc aatatgatcc ggaaaggctc taccagcacc tgcagtcggt ctacgatcgc
     2041 aacgacggcg acgtctccgg ctatctcatc gacgagctca cccagttcgg tctcatcttc
     2101 ccggatgacg ccagggcggc cgacgcctcc tggctgctcg ccaagatcta cctggataaa
     2161 aacaaacggc atgaagccct cgccgccgcc ttcaagaccc tctatctcta cccccgcagc
     2221 ctccacgaag gagaatgccg ggatatcatc cagaacgtcc tcgccaggga ggtggtctac
     2281 gcccccaaaa aaggactgct cgagagtgcc gtctacggca ccgctgtgga tgcagccact
     2341 ctggaacgct ggcacgccta tctcaacctg ctcaaggatc tcgaccagcc caggctctat
     2401 gactggtccc tgaccgaggc cagaaccttc gtcacccgct tcccgggcga ttccctcatt
     2461 tatccggtcc tggaatggac cggcgatctc tatcagcggc tcggcaagat ctgggaggcc
     2521 atctacagct accgcaagct cgaagagatc tttcccgacc acccccagct cccctatgcc
     2581 ctccaccagc aggccctgct cctgagcgag cgcgctgcca agcatgagga cgcggtcagc
     2641 cgcctcaccc gcatcctcgc ccattatccg gaaagcgact tcgcgcccac cgccgcctat
     2701 accatcggca aaatcaagga gagggagctc aaggattata acggcgccat cgaggcctac
     2761 cgcaagctgg tcgcgggatg gcccaaggat gaacgcgccg tcgaggtcct cctcgccatc
     2821 gccgacatcc aggaccgccg gctgaagcag tacgatctcg cccttgccac ctatgacgaa
     2881 atcatcaagg actatgtcag cgatccccgc ggtgcgcgcg cactcgagct caccggcgac
     2941 ctctacgccg aaaacctcaa ggactatctc aacgctgcca gcagctacgc cagggtctcc
     3001 aagctctata ccgccgacga aaaggcgccc gatctgctca tcaaggcggg cgatctctgc
     3061 gcggaacggc tcaaggatta ccgcaaggcc atcgaatact atgagatggt cgccaacctt
     3121 taccctgcca gcaaaaaggt caaggaggcc aacaagcgca tcgacaaagc ctcaaaaaag
     3181 attaccgata acctctgaaa ttctgccccc tccccgcgct gcgcgcgggg agggggcttc
     3241 ccttcctgct gaaaatccca aaattattat tgcatattta tacattgttt tgtatattta
     3301 tgcatgaaac agagaaccga ccgccaccat gccatccggg cgatgatcga atcccgcccc
     3361 atcgccagcc agaaccagct cctcgaggaa ctgcaggcac tgggttttga tgtcacccag
     3421 gcgacccttt cacgcgatct cctgctcctg ggcgcaatga ggattcccga tccggaaaag
     3481 ggctatatct acgccctccc cggcgcggca gcgctagccc cgcccctgag ggacacctcc
     3541 gccctgcacg cctgccgttc tctcgccttt tccggccatc tggcggtggt caagagcctg
     3601 ccctccttcg ccccttccat cggtctgctc ctcgacggcc tcaacctgcc cgaagtggtc
     3661 ggcaccgtcg ccggcgatga cactctcatc gtcgtcctgg ccgagggaac aagccatcac
     3721 cgattcaggc agatcctgct ggagcgcctc cccgagctgc gcgaccgttt ctgatctcga
     3781 tccctaaaaa gcataaatcc tgaacagccc cggcggcgca gactcactca gcccgcacca
     3841 ccggaatctt ttcatcatca tccatcaaga aaggtctgtc atgaaagaga aagtgatcct
     3901 cgcctacagc ggcggcctcg acacctccgt gatcctcaag tggctcgagc tcaagggcta
     3961 tgacgtcatc gcctatgtcg ccgacgtcgg ccagaaggag gactttgagg ctgtccgcga
     4021 gaaagccctc aagaccggcg ccagcaaggt ctacatcgag gatctgaaag aggagctggt
     4081 gcaggagtac atctttgcgg cggcgcgcgg caacgccctt tacgaaggcc gctatttcct
     4141 cggcaccgcc atcgcccgcc ctctcatcgc caagcgtcag atcgagatcg ccgcccggga
     4201 gggagcgcat tatgtttcgc acggcgccac cggcaagggc aacgatcagg tccgcttcga
     4261 gctggcttac tacgccctcc agcccgacat tcaggtcatt tcgccctgga aggatgcgga
     4321 attcctcgcc cagttcaagg ggcgcagcga tatgatcgcc tttgccgaaa aatggtccat
     4381 cccggtcaag gcctcgatcg ccaaatccta cagcgaggac gaaaacctca tgcatatcag
     4441 ccatgaggcc ggcatcctcg aggacccgct cttccgtccc cacgaggaga ttttcagccg
     4501 gacggtcgcg cctcaacagg cccccgatgc ggagaccctg ctcgagatcg agttcgccga
     4561 cggctacccg gtcaaggtga ccaaccgcag cgacagcacg gtcgtcaccg gcgcactccc
     4621 cctcttcacc tatctcaacc gggtcggtgc ggccaacggc gtcggccgcg tcgacatggt
     4681 cgaaaaccgc ttcgtcggga tcaagtcgcg cggcgtctac gaaacccccg gcgccaccat
     4741 cctctgggcc gcccaccgtg acctcgaggg gatcgccatg gacaaggagg tgatgcgcat
     4801 ccgcgacatg ctctcccccc ttttctcgga actgatctac aacggcttct ggttctcgcc
     4861 tgaaatggat tttctcctca gcgcctttga aaagagccag gagaaaatca acggttcggt
     4921 gctgctctcc atctacaagg gcaatgtcac ccccatcggc cggcaatccc cgatctcgct
     4981 ttacaaccag gatctttcga gcatggacgt ggaagggggc tataacgcca tggatgccgg
     5041 gggctttatc cgcatccatg ctatccggct caaggcccac aacatcgtcc tcaagaacgg
     5101 caaaccctac acctggcgcc acaaggagta agatgaaact ttggcagaat cctccggcgg
     5161 ccgctgcgcc gccggatccc ctcacagaac ggatcgagca gttcaccgcc ggtgcggacc
     5221 ccatcctcga cctcgagctg ctgccctggg atggtctcgc ctccaaggcc cacgcccgca
     5281 tgctcgccaa ggtggggctg ctctcccagg aggaggccgc agccctgatt gccggcctgg
     5341 acgggatcct cgccctccac cgcaggggag aattcacgat cagccgggcc gacgaggacg
     5401 gccacaccgc catcgaaaac catctcgtgc agcgctgcgg cgaggccggc ctcaagatcc
     5461 acaccggccg cagccgcaac gatcag
//
LOCUS       DAOPWU010000230         5484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14860_length_5484_cov_3.384601, whole genome shotgun sequence.
ACCESSION   DAOPWU010000230 DAOPWU010000000
VERSION     DAOPWU010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14860_length_5484_cov_3.384601"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKM23_11005"
     CDS             <1..389
                     /locus_tag="PKM23_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNY92042.1"
                     /translation="RMEERTIKRLYYSIAEVSRITGLKPYVLRYWETEFRELRPAKNR
                     AGNRTYRKNDIRLLFVIKRLLYYEKYTIEGARQKLQYLKRSNDSQLSLTLYEAKCQDL
                     IDEIHQELRELAAILDDLHRGVAQSG"
     gene            366..443
                     /locus_tag="PKM23_11010"
     tRNA            366..443
                     /locus_tag="PKM23_11010"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:401..403,aa:Pro,seq:ggg)
     gene            complement(481..1650)
                     /locus_tag="PKM23_11015"
     CDS             complement(481..1650)
                     /locus_tag="PKM23_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92043.1"
                     /translation="MKPAEYHLTENPRLQRWCGGIGLAALLLCAVGYAANPVQFHHSW
                     LAAHFFVISIALGALIFTMILHLTGATWSVPVRRLFENLAAPMPLFFILFLPVLSGVH
                     ELYHWSHGEAVAKDPLLQLKAPYLNQVFFAVRTVIYFAVWTFLACRLRHLSLQQDEGD
                     DHSERLRRTSAAGVILFALTLTFAAFDWFMSLDAHWYSTIFGLYIFSGCVVAGLAAVI
                     LLARHLQSRGHLVETIGIQQYHDLGKLLFAFTVFWAYMAVSQYFLIWYGNIPEETIWY
                     HHRWHGSWKVVSLLLVFGHFLVPFVLLLTRWAKRNLAVLAGFSIWLLFMHWVDLQWLV
                     MPSLHKEGFVFSWMDGTALAGCAGLYLKYLGWLLRRSALAPVGDPRFEEAVDMNR"
     gene            complement(1658..2251)
                     /locus_tag="PKM23_11020"
     CDS             complement(1658..2251)
                     /locus_tag="PKM23_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNY92044.1"
                     /translation="MSKVKVYLIVVAVLLLMACQRGQYSSKPPVHLVPDMDHQPKYKP
                     QSASSFFEDGAAMRLPVSGTVPADRLHEDSLFYRGVDQSGKPVRISPLKAAPELIAAG
                     EGRFRIYCSPCHGGRGEGQGTVIKRGFIPPPLLWEQRLIAEGDGYIFDVISRGVRNMP
                     SHAHLVPASDRWAIVAWVRTLQQGGQARQAGQSTPSN"
     gene            complement(2266..2817)
                     /locus_tag="PKM23_11025"
     CDS             complement(2266..2817)
                     /locus_tag="PKM23_11025"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNY92045.1"
                     /translation="MTDLNHTEGILARYAGPTELLTAARRVAEAGYRKFDCHSPFPIH
                     GMDEAMRLKRSPLGFMVGIAAFAGAGLAILLQGWASAIDYPLIISGKPFFSYQAFMPV
                     TFGLAVLLGAFTAVFGMLALNRLPRFNHAVFHSERFSQVTRDAFFISIESSDLLFDME
                     KTPALLRETGALEVELLSEPPQD"
     gene            complement(2819..4204)
                     /gene="nrfD"
                     /locus_tag="PKM23_11030"
     CDS             complement(2819..4204)
                     /gene="nrfD"
                     /locus_tag="PKM23_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNY92046.1"
                     /translation="MIHQHEQQWIEGQPTFGGLTDQVSALVENKTPRGWYIALAVSSS
                     LTVVLLALIGYLVTRGIGVWGNNIPVAWAWDIVNFVFWIGIGHAGTLISAILFLLRQQ
                     WRTSINRFAEAMTIFAVICALIFPSIHVGRIWFIYWLFPLPNQMSMWPNFRSPLLWDV
                     FAVSTYFTVSLLFWYVGLVPDLATMRDWARTRLKKITLGILALGWRGGNRQWQHYEMA
                     YLLLAGLSTPLVLSVHSVVSTDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVTTLAII
                     VRKVFSLQNIITVRHLENMCKIMLVTGLMVGYAYGVEFFIAWYSGNIYERFTFLNRAF
                     GPYAWAYWIMISCNVLVPQLFWFKKLRTSIPVMFIAALLINVGMWFERFVIIVSSLSR
                     DFLPGAWGYYKPSWVDVGIFAGSFGLFFTLFLLFIRFLPIIAMSEVKGILPQAHAAVD
                     PSAGEHHTRRS"
     gene            complement(4252..>5484)
                     /locus_tag="PKM23_11035"
     CDS             complement(4252..>5484)
                     /locus_tag="PKM23_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNY92047.1"
                     /translation="ICFQPSALLDGRWANNAWLQELPDPVTKIAWDNPARISPALARK
                     HGLANGDLVDIKAGDREITLPVWIMPGQADESITLELGYGRTRAGRVGDGVGFDVGSL
                     RLSGGMGFYSGVSLRSAGGRHVIACAQEFDGMVERPLVREAGLKEFQGDPHFARHDSE
                     IAAQNLWKEHTYDAGFQWGMVIDLTACIGCNACTIACQSENNIPVVGKEQVAIGREMH
                     WIRLDRYFKGGEENPEMVYQPVACQHCENAPCEQVCPVAATVHDHEGLNVMAYNRCVG
                     TRYCSNNCPYKARRFNFFDYTGRMDELKKMAQNPDVTVRMRGVMEKCTYCLQRLNLAK
                     NRARLEGRPLVDGEVMTACQQACPTGAISFGNLLDDSSRVAIARAWARSYALLGELNV
                     RPRTLYAAKIRNRNPELS"
BASE COUNT         1306 a         1568 c         1618 g          992 t
ORIGIN      
        1 ccaggatgga agagagaacg atcaaacgtc tctattactc catcgccgag gtcagtcgta
       61 tcaccggtct caaaccctat gtgttaagat actgggagac cgagttccgc gaactgcggc
      121 cagccaaaaa ccgcgccggc aaccggacct accgcaagaa cgatatccga ttgctgttcg
      181 tcattaaacg ccttctttat tatgaaaagt ataccattga gggtgcgcgg cagaaacttc
      241 agtacctcaa acgcagcaac gattcacaac tgagcctcac tctctatgag gccaaatgcc
      301 aggacctgat cgatgagatc caccaggagt tgagggaact ggccgccata ttggatgatt
      361 tgcatcgggg cgtagcgcag tccggttagc gcacttgact gggggtcaag gggtcgcagg
      421 ttcgaatccc gccgccccga ccagcaaaaa aaaggtcagc agttatctgc tgaccttttt
      481 ttatcggttc atatcgaccg cctcttcaaa ccgaggatcc cccaccggag ccaatgcgct
      541 ccggcgcagg agccagccga ggtattttag atacagccca gcgcatccag ccaaggccgt
      601 cccgtccatc cacgaaaata caaatccttc cttgtgcagt gagggcatca ccagccactg
      661 cagatcaacc cagtgcatga aaagcagcca gatgctgaat ccggcaagca cagccaggtt
      721 gcgcttcgcc cagcgggtca gcagcaggac aaagggcacc agaaaatggc cgaagacgag
      781 gagtaaactg acaactttcc agctcccatg ccagcggtga tgataccata tcgtctcttc
      841 aggaatattg ccataccaga tgagaaaata ttgagaaacg gccatgtagg cccagaaaac
      901 cgtaaaggcg aagagcagtt tgccgaggtc atgatactgc tggatgccga tggtttccac
      961 aaggtggcca cggctttgca ggtgccgggc gagaagaatg accgcagcca gtccggccac
     1021 gacacatccg gaaaatatgt agagtccgaa tatggtcgag taccaatggg catcgaggga
     1081 catgaaccag tcgaaggcag caaaggtgag ggtgagggca aaaaggatga ctcccgccgc
     1141 actggtcctg cgcagccgtt cgctgtgatc gtccccctca tcttgctgca ggctgaggtg
     1201 gcgcagcctg caggcgagga aagtccagac cgcaaagtag atcactgtgc gcacggcgaa
     1261 gaagacctga ttgagatagg gcgccttgag ctgcaagagg gggtctttgg cgaccgcctc
     1321 gccgtggctc cagtggtaga gctcgtgcac acctgaaagc accggcagga agaggatgaa
     1381 gaacaggggc attggtgcag ccagattctc gaaaagacgg cgcaccggca cgctccaggt
     1441 cgcgccggta agatgcaaga tcatcgtgaa aatgagggcg ccgagggcga tggaaatcac
     1501 gaagaaatga gcggccagcc aggagtggtg aaattgaaca ggattggcgg cgtacccgac
     1561 agcgcacagc agcagggcgg ccagaccgat cccgccacac cagcgctgta gccgcgggtt
     1621 ttctgtaaga tggtattctg caggtttcat aatcccgtca gtttgaaggt gtggattgac
     1681 cagcctgacg ggcttgcccg ccctgctgca gcgtccgaac ccaggcgaca atcgcccaac
     1741 gatcggacgc cgggacgagg tgcgcgtgag aaggcatgtt gcgcacgccg cggctgatga
     1801 cgtcaaaaat atagccgtcc ccctccgcaa tcagccgctg ctcccagagc agaggaggag
     1861 gaatgaagcc ccgcttgatc accgtgccct gtccctctcc acgtccaccg tgacaaggag
     1921 aacagtagat acggaaacgt ccctcccctg cagcgatcag ctccggcgcc gccttgagcg
     1981 gcgaaatccg cacgggtttg ccactctgat ccaccccccg gtaaaaaagc gaatcctcgt
     2041 gcaaacggtc ggcggggacg gtaccgctga ctggcagacg catggcggca ccatcctcaa
     2101 agaatgaaga ggcggattga ggcttgtatt tgggctgatg atccatatcc ggcaccagat
     2161 ggacaggcgg cttgctggaa tactgcccgc gctggcaagc cattaaaagc aggactgcca
     2221 caactatcag ataaaccttc actttagaca tatagtcttc tgttcctaat cttgcggcgg
     2281 ttcggacagg agctccactt cgagggcacc ggtttcgcgc agcagggccg gggtcttttc
     2341 catatcgaag aggagatcgc ttgattcgat gctgatgaag aaggcatcac gcgtcacctg
     2401 ggaaaagcgc tcactgtgaa agaccgcatg gttgaaacgc ggcagccggt tgagcgccag
     2461 catcccgaat acagcggtga aagccccgag cagcaccgcc aatccgaatg taaccggcat
     2521 gaatgcctga tagctgaaga agggcttgcc cgagatgatc agtggataat caatggcgct
     2581 ggcccatccc tgcagcagaa tggcgagtcc agccccggca aaggcggcaa tgccgaccat
     2641 gaagcccagc ggtgaacgtt tcagacgcat cgcctcgtcc ataccgtgga tggggaaggg
     2701 agaatggcag tcgaatttgc gatatccagc ctccgccact ctgcgcgccg ccgtcagcag
     2761 ttcggtggga cccgcatatc gggccagaat cccttccgta tggtttagat cagtcatttc
     2821 agctccgcct ggtatgatgt tctcctgccg acggatccac cgctgcgtgg gcctgcggca
     2881 ggattccctt tacctccgac atggcaatga tcggcagaaa gcggatgaag agcagaaaga
     2941 gggtgaagaa gaggccgaat gagccggcaa agatgccgac atccacccac gaaggcttgt
     3001 agtagcccca ggcgcccggt aaaaaatcgc gcgagagtga gctgacgatg atgacaaaac
     3061 gctcgaacca catgccgacg ttgatcagca gcgcagcgat gaacatcacc gggattgagg
     3121 tgcgcagctt tttgaaccag aagagctgcg gcaccagtac attgcagctg atcatgatcc
     3181 agtaggccca ggcataaggg ccgaaggcgc ggttgagaaa ggtgaagcgc tcatagatat
     3241 tgccgctgta ccatgcgata aaaaactcca ccccgtaggc atatcccacc atcaaaccgg
     3301 ttaccagcat gatcttgcac atgttttcaa gatggcgcac cgtgatgatg ttctgcagcg
     3361 aaaaaacctt gcggacgatg atcgccaggg tagtgaccat agcgaagccc gaaaagatcg
     3421 ccccggcgac gaaatagggg ggaaagatgg tggtgtgcca accggggatg acgctggtgg
     3481 caaagtcggt gctgaccaca ctatgcaccg agagcaccag cggggtggag aggccggcga
     3541 gcaggaggta ggccatctcg taatgctgcc attggcggtt gccgccccgc cagcccaacg
     3601 cgaggatgcc gagcgtgatc tttttcaggc gtgtcctggc ccaatcccgc atcgtggcca
     3661 gatcaggcac cagccccacg taccagaaga gcagggagac agtgaagtag gtgctcaccg
     3721 caaagacgtc ccagagcaaa ggactgcgga aattgggcca catcgacatt tggttgggca
     3781 gagggaagag ccagtagatg aaccagatac ggccgacgtg aatgctcgga aagatcagcg
     3841 cgcagatcac tgcgaagatg gtcatcgcct cggcgaaacg gttgatcgag gtgcgccact
     3901 gctggcgcag cagaaagagg atggccgaga tcagcgtgcc ggcgtggccg atgccgatcc
     3961 agaagacaaa gttgacaatg tcccaggccc aggcgaccgg aatgttgttt ccccacaccc
     4021 cgatgcctct ggtcaccagg tagccgatca gggccagaag caccacggtc aggctgctgc
     4081 tcaccgccag ggcgatatac cagcccctcg gggttttgtt ctccaccagc gcactcacct
     4141 gatcggtgag cccgccgaaa gtgggctggc cctcaatcca ctgctgctca tgctgatgaa
     4201 tcaagctcac gcgtcaatcc attcttaggt gaataggcga cagtcctgtc ttcaagagag
     4261 ttcaggattc cggttgcgaa tcttggcggc ataaagggtg cgcggccgta cattgagctc
     4321 cccaagaagg gcgtagctgc gtgcccatgc gcgggcgatg gccacacggc tggagtcgtc
     4381 gagcagattg ccaaaggaaa tcgcgccggt cgggcaggcc tgctgacagg cggtcatcac
     4441 ctcgccgtca accagtgggc gcccctccag ccgggcccgg ttcttggcca ggttcagcct
     4501 ctgcagacaa taggtgcact tttccatcac cccgcgcatg cgcaccgtca catccgggtt
     4561 ttgcgccatt ttcttaagtt catccatccg gccggtataa tcgaaaaagt tgaaacgccg
     4621 cgctttgtag gggcagttat tcgagcagta gcgcgtccca acgcagcggt tgtaggccat
     4681 gacattgaga ccctcatggt catggacggt ggcggccaca ggacaaacct gctcacaagg
     4741 ggcattctca caatgctggc aggccaccgg ctgatagacc atttccggat tttcctctcc
     4801 gcctttaaag taacggtcga gacgaatcca gtgcatctcc cgtcctattg ccacttgctc
     4861 cttgccgacc acggggatgt tgttttcgct ctggcaggcg atcgtgcagg cgttacagcc
     4921 gatgcaggca gtgagatcaa tcaccatacc ccattggaaa ccggcatcgt aggtgtgttc
     4981 cttccagaga ttttgggccg cgatttcgct atcatgacgg gcgaaatgcg gatcgccctg
     5041 aaattccttg aggccagcct cgcgcaccaa cgggcgttca accatgccgt caaactcctg
     5101 ggcgcaggcg atgacatggc ggccgccggc agagcgcagg ctcactccgc tgtaaaagcc
     5161 catgccgccg gagaggcgca gggatccgac atcgaatcca acgccgtcgc cgacgcgacc
     5221 ggcgcgagta cggccgtaac ccagttcgag ggtgatgctc tcgtccgcct gcccgggcat
     5281 gatccagact ggcagtgtga tctcccggtc tccggctttg atatcgacca gatcgccatt
     5341 ggcgagtccg tgtttgcgcg ccagcgcagg gctgatccgc gcgggattgt cccaggcgat
     5401 cttggtgacc ggatcaggca gctcctgaag ccaggcatta ttggcccaac ggccgtccag
     5461 gagggcggag ggctggaaac aaat
//
LOCUS       DAOPWU010000231         5471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14903_length_5471_cov_4.388109, whole genome shotgun sequence.
ACCESSION   DAOPWU010000231 DAOPWU010000000
VERSION     DAOPWU010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14903_length_5471_cov_4.388109"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1426)
                     /locus_tag="PKM23_11040"
     CDS             complement(206..1426)
                     /locus_tag="PKM23_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNY92048.1"
                     /translation="MQRFLPLLLILILLLPLRAQERRDRAVFEIRRDAMLDSITAEMQ
                     KAKPAREPGKLMQLDFSTIAAPVSAAEFKSVWHLPPVLQGLSGMCWCFSTTSMLESEI
                     HRQSGRVIDISELYTVYWEHVEKAREFVRTRGRSVHGEGSEANAVFRIWKKYGCVPAA
                     AYTGLKSGVKFHDHENTLFPEIHSYLESVKTANAWNEETVVATVRAILDHWLGAPPAV
                     VTVEGVKYTPQEYLAKVVRIDPDDYVDLLSLMEKPWYQKVEFTVPDNWWHSADYYNIP
                     LDEFMAAIKSAIRKGYSIEIGGDMSEPGYSRGAAGMAVVPSWDMPSANITDEARQFRF
                     SNGTTADDHGLHLVGYVEKEGVDWYLVKDSWSSAYNSSHPGYYFFHEDYVRLKMLCCS
                     MHKDAAKEVLKRFE"
     gene            complement(1470..2645)
                     /locus_tag="PKM23_11045"
     CDS             complement(1470..2645)
                     /locus_tag="PKM23_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92049.1"
                     /translation="MEVNGIAAMVPMPIQVVIDDVGWWSGEDGSARQEPYRTGIGRNH
                     VPADYQAIADLGKKLGVRPQAATILCEWDRTNMLREVPTSTWMRENWDNRKWTGPWLE
                     EAADIIRSNARHYELTLHGIGHEYWADSHFTRAEWADRSGRMRPLDQVELHLDYYARL
                     LEQNRLGPFPASFVPTAFLHGFGPAAGEEVSMAQVLKRRGIHYINTPFSMMANAGAAQ
                     YGLFGIDDGVITVDRGEDLFDWDVIGGVPEARISGPTCGMHWPNLLHEDPARNAEVVE
                     GWVRYLSAYNSGLATMLAPDSTFFQQQLAHHAGGVVSLNGPMIDLDFAKIQPIPEQIA
                     GRQFMLKISSPFRLRFKPEKIRVLANADALEAEKIIYTLTLERLPGERAARIGLEAA"
     gene            complement(3014..5125)
                     /locus_tag="PKM23_11050"
     CDS             complement(3014..5125)
                     /locus_tag="PKM23_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNY92050.1"
                     /translation="MKHKRVTAAGRLSIVLLLILSGMALAAGTPVWWDNPKGLPNWTQ
                     TIATGTASNTGPQAQYIIAKIDVDNEYREGYSKYVWAQVEWSLVEGTGSFQTGFTDHL
                     IKWLNDDGSCPASPEEDYPDPDGYGFMKCEGDFAPQFGYANGYELSYFKISPQPACER
                     LEIRFEVGPNSRIDYRVEIQTVCINWDFGDAPDPAYPTVQKHDGARHIVTSDCYLGSL
                     IDAEENGQPDAAAAGDDLNGDDEDGVLFADPGHLTQGSPAALTVTATKAGYLNAWVDF
                     NANGSWADPGEQVFTAFAVAAGANSLNFAIPTDAALGVTFARFRFGTDQTLSFTGAAG
                     DGEVEDYQIEILPAEYDFGDASDPTFPTLLASDGARHLKGNLLLGKLIDAEPDGQQNN
                     EAEGDDDGGLDDEDGVLFDENTLITKHHASVIVTASSPGFLNGWVDFNGNGSWADPGE
                     QVFIDKTLVAGENPLTFYIPDSALTTGVASRFRFNSTGKLTPAGAADDGEVEDYIIPI
                     EVPVELSSFTARMVPGGVLLEWTTQSETENLGFHLYRAGSSDDFRQITSQLIPGAGTT
                     AAAHSYSYEDNGVESGRSYRYKLADLDHSGHLQFGAPIEVTVAAPDQFELGQNYPNPF
                     NPSTIIPFKIKQAGFVSLEIYNLKGQMVRALVSRSLQAGSHAVVWDGRDAKGNAVPSG
                     SYIYKITTAAFEQSKRMEFIK"
BASE COUNT          991 a         1617 c         1782 g         1081 t
ORIGIN      
        1 aggtctacga taccctgaaa agcatcggcg cgcagcgggg cgtagacttt ggtctatagc
       61 aacactgaac aaaaaagtaa ttctcgcttc caacccgccc ggggcggcag gccgctcccc
      121 ccaactcgct gccaacccgc ccggggcgag aggccgctcc ccccttctcg ctcccacccc
      181 ggtcccggcg gcgcggccgg aaatcctact cgaacctttt cagcacctct ttggcggcat
      241 ccttgtgcat gctgcagcag agcatcttta gccgcacata atcctcatga aagaaatagt
      301 agccggggtg gctgctgttg tacgccgaac tccacgagtc cttgaccaga taccagtcca
      361 caccctcttt ctccacatag ccgaccaggt gcagcccatg gtcatcagcg gtggtgccat
      421 tgctgaagcg gaactggcgc gcctcatcgg tgatattggc tgatggcata tcccacgatg
      481 gcacgactgc catgccggcg gcgccgcgcg agtagccggg ttcgctcatg tccccgccga
      541 tctcgatcga ataacccttg cggatggcgc tcttgatcgc cgccatgaac tcgtcgaggg
      601 ggatgttgta atagtcggcg ctgtgccacc agttgtccgg cacggtaaac tcgacctttt
      661 gataccaggg cttttccatc agcgagagca gatcgacgta atcgtccgga tcgatgcgca
      721 ccactttggc gagatactcc tgcggcgtgt atttgacccc ttcaaccgtg accaccgccg
      781 gcggtgcgcc gagccaatgg tccaggatgg cccgcaccgt ggcgacgacg gtctcttcgt
      841 tccaggcgtt ggcggtcttg acgctctcca gatagctgtg gatctccggg aagagggtgt
      901 tctcgtggtc gtgaaatttg acaccggatt tgaggccggt gtaggcggcc gcgggcacgc
      961 agccgtactt tttccagatg cggaagacgg cattggcctc cgagccctcg ccgtggacgc
     1021 tgcggccgcg cgtgcgcacg aattcacgcg ccttttcgac gtgctcccag tagaccgtgt
     1081 agagctcgga gatatcgatc acccgcccgc tctggcggtg gatctccgac tcgagcatcg
     1141 aggtcgtaga aaagcaccag cacatccccg acaggccctg caggaccggc ggcagatgcc
     1201 agacgctctt gaattccgcg gcggaaacgg gcgcagcgat ggtcgagaaa tctagctgca
     1261 tcagctttcc gggttcgcgg gccggtttcg ccttctgcat ctcggctgtg atcgagtcga
     1321 gcatggcatc ccggcgtatt tcaaaaacgg cgcggtcgcg gcgctcctgg gcgcgcaggg
     1381 ggagcagaag gatcaggata aggaggaggg gaaggaatcg ctgcatgttt tttttctcct
     1441 gtatggaccg ggtgatctgc acagacaacc tatgccgcct caaggccgat ccgggcggcc
     1501 cgttctcccg gcagccgctc gagggtgagg gtgtaaatta ttttctccgc ttccaatgca
     1561 tcggcattgg cgagcacgcg gatcttttcc ggcttgaagc gtagccggaa agggctgctg
     1621 attttgagca tgaactgccg gccggcaatc tgctccggta tgggctggat tttggcaaag
     1681 tccagatcga tcatcggccc gttgagcgag accacaccgc ccgcatggtg cgcgagctgc
     1741 tgctggaaga aggttgaatc cggggccagc atggttgcca ggccggaatt ataggccgac
     1801 agatagcgca cccagccctc caccacctcc gcattgcgcg ccggatcctc gtggagcagg
     1861 ttcggccagt gcatgccgca ggttggtccg cttatccggg cctccggaac gccgccgatg
     1921 acatcccagt caaagagatc ttccccgcgg tcgaccgtga tcaccccgtc atcgatgcca
     1981 aagaggccat actgcgccgc cccggcgttg gccatcatgc tgaacggggt gttgatataa
     2041 tggatgccgc gccgtttgag cacctgcgcc atgctcacct cctcccctgc agccggaccg
     2101 aagccgtgca gaaaggcggt ggggacaaaa gaggcgggaa aaggcccgag ccggttctgc
     2161 tcgagcagac gcgcgtaata gtcgaggtgg agttcgacct gatccagggg gcgcatgcgc
     2221 ccgctgcggt cggcccactc ggctctggtg aaatggctgt ccgcccagta ctcgtggccg
     2281 atgccgtgca gggtcagttc ataatggcgc gcattgctgc ggatgatatc ggcggcctct
     2341 tccagccacg ggccggtcca tttgcgatta tcccagttct cgcgcatcca ggtgctggtc
     2401 ggaacctcgc gcagcatgtt ggtccggtcc cattcgcaga gtatggtcgc ggcttgcggc
     2461 cgcacgccca gttttttgcc cagatcggcg atggcctggt aatcggcggg cacatgattt
     2521 ctgccgatgc cggtgcgata aggttcctgg cgggcgctgc catcctcgcc ggaccaccag
     2581 cccacatcat cgatgaccac ctggatcggc atggggacca tggccgctat tccattgact
     2641 tccatttgag cccttccttt gcaggccgcc aggcctgtca gggccagtcc cgccccccat
     2701 tgggctgtct gcctgataaa ctcacggcgc ggcatctgtt tcatacattc tccgttctgc
     2761 catcacccgc cggctcgatg gcagacgggt tgtggagagt ccagtgctgc aaacaggttg
     2821 agagggtata ccccggtata ccggctcccg gtttgagttc aaatttaacc aggcgggctg
     2881 tgaaaagcaa gcaaatacct catcgggact ggaatgaggc acccggagcg atggggtgag
     2941 aggagaaaaa aggagggggc gctgctgcga taaccctggg ggaggccgcg cagcagcgcc
     3001 ttgtgtggga ctcttatttg atgaattcca tccgcttcga ctgctcgaaa gcggcagtgg
     3061 ttattttata gatgtaggag ccgctcggta cggcattgcc cttggcgtcc cggccgtccc
     3121 agaccacggc gtgcgaaccg gcctgcaacg aacggctgac cagcgcgcgc accatctgac
     3181 ccttcaggtt atagatctcg aggctgacaa aaccggcctg tttgatcttg aaggggatga
     3241 tcgtggaggg attgaaggga ttgggatagt tctgtcccag ctcgaactgg tcgggcgccg
     3301 ccacggtgac ctcgatgggc gcgccgaact ggagatggcc gctgtgatcg agatccgcca
     3361 gcttgtagcg gtagctccgg ccggactcga ccccgttgtc ctcatagctg tagctgtggg
     3421 cggctgcagt ggtgccggcc cccgggatga gctggctggt gatctggcgg aaatcgtcag
     3481 aggatccggc gcggtagaga tggaagccga ggttctcggt ctcggactgg gtggtccatt
     3541 cgagcaggac gccccccggc accattctgg cggtgaagga ggagagttcc accggaacct
     3601 cgatcgggat gatgtaatcc tcgacctcgc cgtcatcggc ggcaccggcg ggagtgagct
     3661 tgccggtcga attgaagcgg aagcggctgg ccaccccggt ggtcagggcg ctgtcgggta
     3721 tgtaaaaggt gaggggattt tcacccgcga cgagtgtttt gtcgatgaaa acctgctcgc
     3781 cgggatcggc ccagctgccg ttgccgttga aatcgaccca gccgttgaga aaaccgggcg
     3841 aggaagcggt gacgatgacc gaggcgtgat gcttggtgat gagggtgttc tcgtcgaaga
     3901 ggacgccgtc ctcatcatcc agtccgccgt catcgtcgcc ctcggcctca ttgttctgct
     3961 ggccgtccgg ctcggcgtcg atgagcttgc cgagcaagag gttgcccttg agatggcggg
     4021 cgccgtcgct ggccagcagg gtcggaaagg tcgggtccga agcgtcgcca aagtcatact
     4081 cggccggcag gatttcaatc tgatagtcct cgacctcgcc gtcgcccgcg gcgccggtga
     4141 aggagagggt ctggtcggtg ccgaagcgga agcgggcgaa ggtaacgccc agggccgcat
     4201 cggtcgggat ggcgaaatta aggctgttcg ccccggcggc gacagcaaaa gcggtgaaga
     4261 cctgctcgcc gggatcggcc cagctgccgt tggcgttgaa atccacccag gcgttgagat
     4321 agcccgcctt ggtggcggtg acggtgaggg cggcggggct gccctgggtg aggtggccgg
     4381 gatccgcaaa gaggacacca tcttcgtcat ccccgttgag gtcgtcgccg gcggcggccg
     4441 catccggctg cccgttctcc tcggcgtcaa tcaggctgcc gagatagcag tcgctggtga
     4501 cgatgtggcg ggcgccgtcg tgtttctgca cggtcggata ggcgggatcc ggggcatccc
     4561 cgaaatccca gttgatgcag acggtctgga tctcaacccg gtagtcgatg cggctgttgg
     4621 ggccgacttc gaagcggatc tcgaggcgct cgcaggcggg ctggggcgag atcttgaagt
     4681 aggagagttc gtaaccgttg gcatagccga actggggggc aaaatcgccc tcgcatttca
     4741 taaagccgta gccgtcggga tccggatagt cctcctcggg actggcggga cagctgccgt
     4801 cgtcattgag ccacttgatg agatgatcgg tgaagccggt ctggaaactg ccggtgcctt
     4861 caacgaggct ccactccacc tgggcccaga catacttgga atagccctcg cggtactcgt
     4921 tgtcgacatc gatcttggcg atgatgtact gcgcctgggg gccggtgttg gaggccgtgc
     4981 ccgtggcgat ggtctgggtc cagttgggca gccctttcgg attgtcccac cagaccgggg
     5041 tgccggcggc caaagccatg ccgctgagga tgaggagcag gacgatgctc agcctgcccg
     5101 ctgcagtgac tctcttgtgc ttcatcagct ctcctccaaa aatatgacag gtgtcatgat
     5161 taggttactg gttcgccggc gttcagcgcc cccggcgcgt tatttctgcg cttattttct
     5221 ttatttacaa taccagtaat cagagactcc cttgacacgc aggggattac gcaaaccctg
     5281 tgccagaaaa gttcctctgg tgcccacccg ggctttcgcg ccagccggga gccgggtggg
     5341 gacgagatgg atggggggcc cgcctgcgcg ccagccggga gccgggtggg gacgagattt
     5401 gtaccaggcc gggtgggcga gacgcaaacg agcgagcggg agtggcgaga atgcggaggc
     5461 gaccgggcgc a
//
LOCUS       DAOPWU010000232         5444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_14995_length_5444_cov_6.020598, whole genome shotgun sequence.
ACCESSION   DAOPWU010000232 DAOPWU010000000
VERSION     DAOPWU010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14995_length_5444_cov_6.020598"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="PKM23_11055"
     CDS             <1..1406
                     /locus_tag="PKM23_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92051.1"
                     /translation="QQIQLSSGPEDYPVMWNSRGEIYYYSPSIDTTRFYRTNIEGGKP
                     VLVKVFSGKEPLPADVTSDLSYFVYCNTPENRGITLFDTQTLQHKTITADSGFTQARF
                     SLNSKYLAAGSKVGSDKAVHVFTTGIGGDEIKGPVKIIGSPALKYSWSWTRNDELSFG
                     QRTGFEQLQVCDPQGQPVNPLAGFQSDEKYPSWSPDDQTVVFSSDVASKDQLWLANAV
                     GGQVTQLTAIDDKAISHIWPSWSQDGSQIVYVRVYHMSDYFGQQIWAIDVPTQKHKQV
                     SDYEGRFASLVYSPDGTRLYFSYTSPAQRHIPDHSIPGVRPGNNALVELALTDKSIRI
                     LAQDSLFQFKPFGFTPDASKMLFQKSNYESQSSIWCYSAQGVQQKIISSDTSYLMING
                     ITPDSKFLHVSTWNQKSEREFLTVSIEDGTMRQDTFQKKELWPLRWSHDNQRILCKKD
                     NQITEFWLARNITAAFK"
     gene            1573..3855
                     /locus_tag="PKM23_11060"
     CDS             1573..3855
                     /locus_tag="PKM23_11060"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNY92052.1"
                     /translation="MQKTSASLAALVILTLAGLIFAQQGFQRTPDFLPVSVWYSGGIA
                     RAPMLSTITPGSREQWRRDLAQIKSLGFNTVRTWVEWAHCEPRPGEYHFENLDLLCEL
                     AREQGLRVICQMYIDSAPDWVAKRFPDGLFEAQSGDKVTPQSAPGCCTDHAGVQEAVL
                     TFFTETAKRAMKHPNFHGWDLWSEPHIINWAYINYVPNATFCYCPHTMQVFQEWLQKK
                     YSTLDALNQAWYRNFTAWSEVSAPRFGTILSYTDFIDWKNFIYEKLAHDLRLRYEAIR
                     VADPIHVITSHAAVPSLFYSPYVGAGATDDFLMSEQIDYYGTSLYPKHNHPSTHWERW
                     RFQAAVDFSRSANKRNGGFYVGELQAGKGTYGLNIGNPITPADHRIWMWSAIAKGAKA
                     INIYAYYPMSSGYESGGYGLINLDGSITERAAAAGKTASIITDNMKLLLESKPSPVQI
                     AIVYNPLAQMVGGEQRTVTTDLHQQSLLGWYRFFTERNIPVDFIHRRDLENGDLAEYK
                     LIIIPYPLMFTEKAAEGLKNYVAAGGFAVSEARMAWNDERGFAAPAIPGMGLDQVFGV
                     RESSVKMGESPAMGVCNLTHPLMQGFTTADTLHGANFAESVVPLSGGAAKVLARFADA
                     TAALAAGKYGKGETLFIGSFLGMANHIRADRANERLLWNIVRWSSIERPFTSSHDGNK
                     DAPVEIRLRLTAAGSILFLINHSEADQQMTVDLRVAKSGRYRLRDLVHDQQLDLTARG
                     RELKIGHELKARDVQVWVIE"
     gene            complement(3919..4392)
                     /locus_tag="PKM23_11065"
     CDS             complement(3919..4392)
                     /locus_tag="PKM23_11065"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNY92053.1"
                     /translation="MQIIIDGYNVLLRGRRAGAIGAPELERMREELTRKAAGYAASRD
                     IRITLVFDGQNGMGVQAAPPPGKVKVVFSRPPENADAVIKKMVQGHEQPRNIVVVTSD
                     QPLARFVQSCGCQLLSSEAWRQKMEHGRDEDLAEKHGRLNDLNIEEWLRIFGEKK"
     gene            4624..>5444
                     /locus_tag="PKM23_11070"
     CDS             4624..>5444
                     /locus_tag="PKM23_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNY92054.1"
                     /translation="MPYQGEIAALTAVVLWSSTAILFETIGKRIGSFNTNLMRLTLAA
                     LLLSLTLYFQKGMFLPVTASNDQILWLGLSGVVGLALGDAAFFKCLVVLGSRLATLLL
                     SLSPVFTTIFAWFFLGEHLSLVGLAGILTTFTGIFWVVNEKSEEHHPHVKFKGIILGI
                     LAALGQAAGVILAKYGFRTEIDALSATILRMVPAALVMWTAAILLKRLTPMKIFITDK
                     KATRFIIIASIIGPYLGVWLANVAVKYAEAGVASTLLAMVPVLLIPLVWIVHNTRP"
BASE COUNT         1183 a         1615 c         1455 g         1191 t
ORIGIN      
        1 agcagcagat acaactttcg tcgggtccag aggactatcc agtgatgtgg aatagcagag
       61 gcgagattta ctattattca ccatccatag acacaacccg gttctacagg acgaacatcg
      121 aaggtggtaa accggtgctg gtcaaggtat tctccggaaa agagccgctt cccgctgatg
      181 ttacgtcgga tttatcctat tttgtctatt gcaatacccc ggagaacagg ggtattactc
      241 tttttgatac ccaaactctc caacataaaa ctattacggc agattcgggt tttacgcaag
      301 ccagattttc actcaacagc aaatatctcg cggcaggaag caaagtcggc agcgataaag
      361 ctgtgcatgt ttttaccacc ggaattggcg gggatgaaat aaaaggacca gtgaaaatca
      421 tcggctcgcc tgcgctgaaa tacagctgga gctggacgag aaacgatgaa ttatcttttg
      481 gccaaaggac agggtttgag cagttgcagg tgtgcgatcc ccagggtcag cctgtcaatc
      541 ctctggcagg ttttcaatct gatgaaaagt acccttcctg gtctcctgat gatcagacgg
      601 ttgtctttag ctcggacgtc gcaagcaagg atcaactttg gctggctaat gcggtgggcg
      661 gtcaagttac tcaattaact gcaatcgatg ataaggcgat atcacatata tggccttcct
      721 ggtcccagga tggctcacag atcgtctacg tccgtgtata tcatatgagt gactattttg
      781 gccagcaaat atgggctata gatgtaccga cgcaaaagca caaacaggtc tcggattatg
      841 aaggcaggtt tgcctccctg gtctactcac ctgatggaac caggctctat ttctcgtaca
      901 cttctccggc gcaaaggcat attcccgatc attctattcc gggcgtaagg ccaggcaata
      961 atgccctggt cgagttggct ctgacggaca aatcaatacg gatactggct caggactctt
     1021 tgttccaatt caaacccttt ggattcacac ctgatgcaag caaaatgctc tttcagaaat
     1081 ccaactatga aagtcagagc tcgatttggt gctatagtgc gcaaggcgta caacagaaaa
     1141 taatttcatc cgatacgagc tatcttatga tcaacgggat aacacccgat tcgaagttcc
     1201 tccatgtgtc aacctggaat caaaagagcg aacgagaatt tctgactgtg agtattgaag
     1261 atggcaccat gcgacaggac acttttcaga aaaaagagct gtggcctctc agatggtctc
     1321 atgacaatca gcgtattctg tgtaaaaaag ataatcagat caccgaattt tggctggcca
     1381 ggaatatcac ggcagccttt aaatgacgat tggttttgac gcctgcgtgc cggcataaaa
     1441 ctggtgcgca ggcggatctc tccgttcctc cgccctgccc tcctcctgct aaatccttgc
     1501 gcaggtaaac aaaaattgct acattgcatc agttcaccct tacatcctct tgctcaatac
     1561 aggagaaatg ttatgcagaa aacctctgcc tccctggcgg ccttggtcat tctcaccctt
     1621 gccgggctga ttttcgccca gcagggtttt cagcgcacgc ccgatttcct gccggtctcg
     1681 gtctggtaca gcggcggcat cgcccgcgcc ccgatgctct caaccatcac ccctgggtcc
     1741 cgggagcagt ggcgccgcga tctggcgcag atcaaatccc tcggcttcaa caccgtgcgc
     1801 acctgggtcg agtgggccca ctgcgagccg cgaccggggg aataccattt cgagaacctc
     1861 gacctcctct gtgaactggc ccgggagcag gggctgcgcg tcatctgcca gatgtacatc
     1921 gactcggcgc cggattgggt ggccaagcgc ttccccgacg gcctcttcga agcccagagc
     1981 ggcgacaagg tcacgcccca gtcagccccc ggctgctgca ccgaccacgc cggggtgcag
     2041 gaagcagtgc tcaccttttt caccgaaacc gccaaaaggg cgatgaaaca tcccaacttt
     2101 cacggctggg atctctggag cgaaccccac atcatcaact gggcctatat caattatgtc
     2161 cccaacgcca ccttctgcta ctgcccccac accatgcagg ttttccagga gtggctgcag
     2221 aaaaaatatt ccactctcga tgccctcaac caggcctggt accgaaattt caccgcctgg
     2281 agcgaggtga gcgccccgcg cttcggcacc atcctcagct ataccgattt catcgactgg
     2341 aaaaatttca tctacgaaaa gctggcgcat gacctgcgcc tgcgctatga ggcgatccgg
     2401 gtggcggacc cgatccacgt catcacctcc cacgcggccg tgccctcgct cttttactcg
     2461 ccctacgtcg gcgccggggc caccgacgat tttctcatgt ccgagcagat cgattattac
     2521 ggcacctcgc tttatcccaa gcacaaccat ccctccaccc actgggagcg ctggcgcttc
     2581 caggcggcgg tcgatttctc ccgcagcgcc aacaagcgca acggcggctt ttacgtcggc
     2641 gaactgcagg cgggcaaggg cacctacggc ctcaatatcg gcaatccgat cacccccgct
     2701 gatcaccgca tctggatgtg gtcggccatc gccaaggggg ccaaggcaat caatatctat
     2761 gcctattatc cgatgtcctc gggctacgaa tcgggcggct atggcctgat caacctcgat
     2821 ggcagcatca ccgagcgggc ggccgctgcc ggcaagaccg ccagcatcat caccgataac
     2881 atgaaactgc tgctcgaatc gaaaccgtcg ccggtgcaga tcgcgatcgt ctacaatccc
     2941 ctcgcccaga tggtcggcgg cgagcagcgc accgtcacca cggacctgca tcagcagtcg
     3001 ctgctcggct ggtaccgctt tttcaccgag cgcaacatac cggtcgattt catccaccgc
     3061 cgtgacctgg agaatggcga tctggcggag tacaaactga tcatcattcc ctatcccctg
     3121 atgttcacgg aaaaggcggc ggagggtttg aagaattacg tcgccgcggg cggattcgcc
     3181 gtgagcgagg cgcggatggc ctggaatgac gagcgcggct ttgcagcgcc ggccattccc
     3241 gggatggggt tggaccaggt cttcggggtg cgcgaaagct cggttaaaat gggtgagagt
     3301 ccggccatgg gggtttgcaa cctgacccac ccgttgatgc agggattcac gaccgctgac
     3361 accctgcacg gagccaattt tgccgaatcg gtcgtgccgt tgtccggggg cgccgccaaa
     3421 gtgctggccc gcttcgctga cgctacggcc gcgctggccg ccgggaaata tggcaagggc
     3481 gagaccctct ttatcggttc gttcctcggc atggccaacc acatccgggc cgaccgtgcc
     3541 aacgaacgcc tgctctggaa tatagtgcgc tggagcagca tcgagcggcc tttcaccagt
     3601 tcgcacgacg gcaacaagga tgccccggtg gagatccgcc tgcggctgac agccgcagga
     3661 tcgatcctgt ttttgatcaa tcacagcgaa gcggatcagc agatgacggt cgacctcagg
     3721 gtggcgaaat cggggagata cagattgcgg gatctggtgc acgaccagca gctggatctt
     3781 acagcgcgcg gccgggagct gaagatcggg catgagctca aggcgcggga tgtacaggta
     3841 tgggtgattg aatagaggag ggcaggttca aggaagtggc acagcggacg aagaaatgca
     3901 tcaaatcgca tcagcggatc actttttctc accgaaaatt cgcagccact cttcgatatt
     3961 tagatcattc agccggccgt gtttttcggc cagatcctcg tcacggccgt gctccatctt
     4021 ttgccgccag gcctctgagg agagcagctg gcagccgcag gactggacga aacgcgccag
     4081 cggctggtcg gaggtgacca ccacgatatt gcgcggttgt tcgtgcccct gaaccatctt
     4141 tttgatcact gcatccgcat tttccggagg gcgggaaaat accaccttga ccttgccggg
     4201 cggaggggcg gcttgcaccc ccatcccgtt ctgaccatcg aaaaccaggg taatccggat
     4261 atccctggag gcggcatatc cggccgcctt gcgagtcaac tcctcccgca tccgctcaag
     4321 ctcgggcgcg ccgatcgcac cggcgcgccg cccgcgcagc agaacattgt agccatcgat
     4381 gataatctgc acctgcaatc catcccgttt tggtcttctt cccccattgt aacctatttg
     4441 gcgaaatatt gcaagggctt ttttcagagc gctctgctcc ccgcgtccac ctgccagcca
     4501 tcctggctaa ccagatgaaa tcagaagaaa acagctttta tatggtttca ttattgatta
     4561 tatttctgga tattctgcag atcgaaagcc ggttggggat tggtttaacg aaagggtatc
     4621 tctttgccat accaaggtga aatcgccgcc ctgaccgcgg tcgtgctgtg gagcagcacc
     4681 gcaatcctct tcgagactat cggcaaacgc atcggctcat tcaacaccaa cctgatgcgt
     4741 ctgaccctgg ccgcactgct cctcagcctg acgctctatt ttcagaaggg catgttcctg
     4801 ccggtgaccg cgagtaatga ccagatcctg tggctgggcc tgagcggagt ggtggggttg
     4861 gcactgggtg acgcggcctt tttcaagtgc ctggttgtgc tcggctcgcg tctggcgacc
     4921 ctgctgctct ccctctcccc cgtcttcacc acgatcttcg cctggttctt cctcggggag
     4981 catctcagcc ttgtgggcct ggcgggcatt ctcaccactt ttaccggcat cttttgggtg
     5041 gtcaatgaaa aaagcgagga acaccatccc catgtcaagt tcaaggggat catcctgggc
     5101 atcctggccg ccctgggtca ggccgccggt gtgattctgg ccaagtacgg ctttcgcacc
     5161 gagatcgatg ccctctcggc gaccatcctg cgcatggtcc cggccgccct ggtgatgtgg
     5221 acggccgcta tcctgctcaa aaggctgact cccatgaaga ttttcatcac ggacaaaaag
     5281 gccacccgtt tcatcatcat cgcctcgatc atcggcccct atctgggtgt ctggctggcc
     5341 aacgtcgcgg tcaagtacgc cgaggccggc gtcgcctcca ccctgctggc gatggtgccg
     5401 gtgctcctca tccccctggt ctggatcgtc cacaacaccc ggcc
//
LOCUS       DAOPWU010000233         5430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15050_length_5430_cov_4.805395, whole genome shotgun sequence.
ACCESSION   DAOPWU010000233 DAOPWU010000000
VERSION     DAOPWU010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15050_length_5430_cov_4.805395"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..502)
                     /locus_tag="PKM23_11075"
     CDS             complement(179..502)
                     /locus_tag="PKM23_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92055.1"
                     /translation="MEALIPIVLFGLVAWVIKFSSDNKLKRYMLDKGVTAETMQMMFS
                     QPVRDNTPASLKWGLVLTAVGLGAFIGLNFADGQDEYVLASMLLCGGLALIVYYIIAS
                     RFTGK"
     gene            666..1265
                     /locus_tag="PKM23_11080"
     CDS             666..1265
                     /locus_tag="PKM23_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNY92056.1"
                     /translation="MEDRDDKIVIESILAGEQSDFASLLERYQKLVVHLVARMIPRAA
                     DREDLCQEIFLRIYRSLGEFQYKSRFSTWVAAIAHNTCINYLQKKRVPLYQDLAATSA
                     EEEDEEVRRVEWVPSSGPSPLERAEASDLRITLEREIEALPLPYRTILTLFHLEEMSY
                     AEIGEITGLPEGTVKNYLFRARRFLRDRLAAHYPVEATS"
     gene            1262..1792
                     /locus_tag="PKM23_11085"
     CDS             1262..1792
                     /locus_tag="PKM23_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92057.1"
                     /translation="MTDHLRDAELQLYLDHQLADRAKLDHLRGCPLCQERLDAYEPLF
                     AALGAAPAWKPSPALPEKVMRRIRKEGVDPLHENLLHTILIIGGLIMAISLSLPYMQA
                     GEYLQEARRFKWPEFNLSWEWLRELNFLPRLAEMSSRIALPGNTILMFAGALLLVLLA
                     DQIITFARGHQPPRSR"
     gene            2191..2865
                     /locus_tag="PKM23_11090"
     CDS             2191..2865
                     /locus_tag="PKM23_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNY92058.1"
                     /translation="MIREPFSWQLPVQRPTKILCLGRNYRAHAAELNNTVPEAPMYFA
                     KVPSSLLPHQGHIRIPAGVGRVDHELELALVIGKRGANIPESKAMEHVAGYTIANDIT
                     AREMQREEQKKGKPWTLAKGMDTFCPLGPWLVPAGSVADPHNLTLELKVDGEVRQKGS
                     TADMVYGIPELIAYISRYMTLEPGDLLLTGTPEGVGPIQPGNRIEGSIDGLGTLENGV
                     VQAGSD"
     gene            2865..3917
                     /locus_tag="PKM23_11095"
     CDS             2865..3917
                     /locus_tag="PKM23_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNY92059.1"
                     /translation="MPGLFAPYGPDFGRLRTTLMGGKADRVPLMELGIDEGVMGQFLG
                     RPLQNLQDKIDFYRLAGYDYIKLAPVINMNPGAAVPQGGLRRSEATDLDRARNWGTES
                     KGVITSWEEFERFEWAEVSDSALRWFDEAEKIMPPEMRVIGQYGDIFTFTWEFMGFET
                     FSYALVEDPALVEAVFERVGSIIFALFERMAQYETVGGLFYSDDIAYFSGLMISPPTL
                     RRYLFPWMKKIGDLCRARPMPCLYHSDGRLWEVIDELIGLGVTTLHPIEPKAMEIREV
                     QAAFGPRLGLIGSVDMDLLARGEPEVIRSTVRNLIEEVGRRGGYCVGSGNSIPNYIPV
                     TNFRAMLETTWEFGQL"
     gene            3939..5420
                     /locus_tag="PKM23_11100"
     CDS             3939..5420
                     /locus_tag="PKM23_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNY92060.1"
                     /translation="MSLYQPIKRLVRHSAIYGIGHILSRSVGFFLLPLYTNLFSTGGF
                     GVAGLAMTWLTVFTLLYAYGLDAGFMRFYVLASQERERRAIFSTAMWTLSLSSLFFSL
                     LLVLFAPRLVALFFGADARTAGVDLVMIIRLLAGILFCDTAAFIPLLLLRADEKSWLY
                     VLIKTGSAMVLLAANLYCILHLKMGVEGLFAANLAASALTLLALVPLLLRSGGGHFSR
                     DWLRQLLRFGLPFLPTGLAIALLDSADRLLLERLDSVAAAGLYSAGARVGMVMGLLVA
                     AFRFAWQPYFLATSREKEAKKIFSRILTYMVAACLTLFLLMSLFIDELLRLRVGGYSF
                     FGEPFWASARVVPPIMLASLFYALYLVFEAALYLEKKSGLIALVTIAGVGVSLALNLL
                     LIPQWGPLGSAWARVIAYACMAMGLFVFAQRCYPVPWEWSRLLQLCLSAALIYALGKL
                     PLLHPAVAVRLALFAAWPLLLAAAGFFRAEEREIIRGFFRKIM"
BASE COUNT          933 a         1781 c         1578 g         1138 t
ORIGIN      
        1 tgacggggga gatttacgaa atttttacca gcaaatcaag agtttttacg agcagggtac
       61 cccccggaaa tctctttccc aataccttgc gcgtgtcccc ctgcagtctg gtgcggaaaa
      121 gcctaaaaaa aagccccccg gggaaacggg gggcatggtg tggagagcct gggcctgcct
      181 atttgccggt gaagcgcgag gcgatgatgt aatagacgat cagggcgagg ccgccgcaga
      241 gcagcatgct ggcgaggaca tattcatcct gcccatcggc gaaattgagg ccgataaagg
      301 cgcccagacc gacggcggtc agcaccagac cccacttgag cgaggcgggg gtgttgtcgc
      361 gcaccggctg gctgaacatc atctgcatgg tctctgcggt gacgcccttg tcgagcatgt
      421 agcgtttcag cttgttgtcc gagctgaatt tgatgaccca ggcgaccagt ccgaacaata
      481 cgataggaat gagcgcttcc atggttttct cctttctggt tggggggact ctttctacta
      541 catccgacag tcatggcgcg gaaatgttgc agccggcagg aaattttctt gaatatttgc
      601 taaaaaatgt gcttcttgta gaagagtgca accttttccc tcttcatctg tctaatggga
      661 ttagaatgga agaccgggat gacaaaatag tgatcgaatc gatcctcgcc ggagagcagt
      721 ccgatttcgc ttctctgctc gagcgctacc agaaactggt tgttcatctg gtggcgagga
      781 tgatacccag ggcagcggac cgcgaggatc tctgccagga gatctttctg cgcatctatc
      841 gaagcctggg cgaattccag tacaaatccc ggttttccac ctgggtggcc gccatcgccc
      901 acaacacctg catcaattat ctgcaaaaga agcgcgtgcc cctttatcag gatctggcgg
      961 cgacgagcgc ggaagaggag gacgaggagg tgcgccgggt ggaatgggtg ccctcatccg
     1021 gaccctcgcc gctcgagcgc gcggaggcga gcgatctgcg catcaccctg gagcgcgaaa
     1081 tcgaggccct ccccctgcct tatcgcacca tcctgaccct tttccatctc gaagagatga
     1141 gctacgccga gatcggcgag atcaccggcc tacccgaggg cacggtcaaa aactatcttt
     1201 ttcgtgcgcg gcgctttctg cgcgatcggc tcgccgcgca ctatcctgtg gaggccacat
     1261 catgaccgac catttgcgcg atgcagagct gcagctctat ctcgaccacc agttggcgga
     1321 cagggccaaa ctcgaccatc tccgcggctg tcccctttgc caggagcggc tcgacgccta
     1381 cgaacctctc ttcgccgctc ttggggctgc accggcctgg aaaccctccc cggccctgcc
     1441 cgagaaggtg atgcgccgga tccgaaagga gggcgtcgat cccctccatg agaacctgct
     1501 ccacaccatt cttatcatcg gcggtctgat catggccatc agcctctccc tgccctatat
     1561 gcaggccggg gagtacctgc aggaggcgcg cagatttaaa tggccggaat tcaatctctc
     1621 ctgggagtgg ctcagggagc tcaatttcct gccgcgcctc gccgagatga gcagccgcat
     1681 cgccctcccc ggcaatacga ttctgatgtt tgccggtgcg cttcttctgg tgctgctcgc
     1741 ggaccagatc atcaccttcg cccggggcca tcaaccgccc cgcagccgct gaggccaccg
     1801 ccccggccgt tgttccctcc tccccggagg ggcctccgcc ggggcttttt ttatggcccc
     1861 gggtctcccc gcgcggccca tatttttctt gcttattatt cccaggtttg atatacttga
     1921 ccaccagacc ggaaatactc accaggagga tcggagatga aggccttttc ctatctgacc
     1981 accaaagggg aaccccgtat cggcgtgcaa acccccgagg ggctgctcaa tttcacccag
     2041 ctctggggct atttcaagga gttgaagaat tttccccggg ctccggagct gccctttctc
     2101 cagctcatgg ttgagctgga ttatttttcg gaggagacct tcgccgaggt catcgccgcg
     2161 gtcagggagt atcgcagcct cgatgacctc atcatcaggg agcccttcag ctggcagctc
     2221 cccgttcagc gcccgaccaa aatcctctgc ctcggccgca actatcgcgc ccacgccgcc
     2281 gaactcaaca acaccgtccc ggaagccccg atgtacttcg ccaaggtgcc ctcgagcctg
     2341 ctgcctcatc agggccatat ccgcatcccg gccggcgtcg gccgcgtcga tcatgaactc
     2401 gaactcgccc tggtgatcgg taaacgcggc gccaatatcc ccgagagcaa ggccatggag
     2461 cacgtggccg gatataccat cgccaacgac atcaccgccc gcgagatgca gcgcgaggag
     2521 cagaagaagg gcaaaccctg gaccctggcc aaggggatgg acaccttctg ccccctcggc
     2581 ccctggctgg tcccggccgg cagcgtggcg gatcctcaca acctcaccct cgaactcaag
     2641 gtcgacggcg aggtgcgcca gaaggggagc accgccgaca tggtctacgg gatccccgag
     2701 ctgatcgcct atatctcccg ctacatgacc ctcgaacccg gcgacctcct cctgaccggc
     2761 acccccgagg gggtgggccc gatccagcct ggcaaccgca tcgagggcag catcgacggc
     2821 ctcggcaccc tggagaacgg ggtggtccag gcaggatccg actgatgccc ggtctcttcg
     2881 ctccctatgg ccccgatttc ggccggctgc gcaccaccct gatgggcgga aaagcagatc
     2941 gtgtccccct gatggaactc ggtatcgacg agggggtcat gggccagttc ctcggccgcc
     3001 ccctgcagaa tttgcaggac aagatcgatt tctatcgcct ggccggctat gactatatca
     3061 agctcgcccc cgtcatcaac atgaaccccg gagcggccgt gccgcagggg gggctgcgcc
     3121 gttccgaggc caccgacctc gaccgcgccc gcaactgggg caccgagagc aagggggtca
     3181 tcacctcgtg ggaggagttt gaacgctttg agtgggcgga ggtgagcgac tccgccttgc
     3241 gctggttcga cgaggccgag aagatcatgc cccccgagat gcgggtgatc ggacaatacg
     3301 gcgacatttt caccttcacc tgggagttca tgggcttcga gaccttcagc tacgccctgg
     3361 tcgaggatcc cgcactggtg gaggcagtct ttgagcgggt cggctcgatc attttcgccc
     3421 tctttgagcg catggcgcag tacgagaccg tcggcgggct cttttacagc gacgatatcg
     3481 cctacttttc cggcctgatg atctcgccgc ccaccctgcg ccgctacctc tttccatgga
     3541 tgaaaaagat cggcgacctc tgccgggcga ggcccatgcc ctgtctctat cacagcgacg
     3601 gcaggctctg ggaggtgatc gatgagctga tcgggctggg ggtgaccacc cttcatccga
     3661 ttgagcccaa ggcgatggag atccgcgagg tgcaggccgc tttcggaccg cgcctggggc
     3721 tgatcggcag cgtcgacatg gacctgctgg cgcgcggcga gccggaagtg atccgttcca
     3781 ccgtgcgcaa cctgatcgag gaggtggggc ggcgcggcgg ctactgcgtc ggctccggca
     3841 acagcatccc caactatatc ccggtgacca actttcgcgc gatgctcgag accacttggg
     3901 agttcggcca gctctagcac ccaaccttgc ggcggatcat gtccctctat cagcccatca
     3961 aacgcctggt caggcattcc gccatctatg gcatcggcca tatcctcagc cgttccgtcg
     4021 gtttcttcct gctgcccctc tataccaatc tcttttccac aggcggcttc ggcgtcgccg
     4081 gcctggccat gacctggctg acggtcttca ccctgctcta tgcctatggt ctggatgcgg
     4141 gctttatgcg gttttacgtc ctcgccagcc aggagcggga gcggcgcgct atcttttcca
     4201 cggcgatgtg gaccctctca ctctcgagcc tcttcttctc cctgctgctc gtcctctttg
     4261 cgccccggct ggttgccctc tttttcggcg ccgatgcccg cacggccggg gttgacctgg
     4321 tcatgatcat ccgcctcctc gccggcatcc tcttttgcga taccgccgcc tttatccccc
     4381 tgctgctgct gcgcgcggat gaaaagtcct ggctctacgt cctcatcaag accggctcgg
     4441 ccatggtgct tctcgccgcc aacctctatt gcatcctcca cctgaagatg ggggtggagg
     4501 gcctgttcgc tgccaacctt gccgcttcgg ccctgaccct gctcgccctg gtgcctcttc
     4561 tcctgcgcag cggcgggggg catttttcca gggactggct gcgccagctc ctccgcttcg
     4621 gcctcccctt cctgcccacg gggttggcca tcgccctcct cgacagcgcc gaccgcctgc
     4681 tgctcgagcg gctcgattcg gtcgcggcgg ccggccttta cagcgccggg gccagggtgg
     4741 ggatggtgat gggactgctg gtggctgcct tccgcttcgc ctggcagccc tattttcttg
     4801 ccacctcgcg ggagaaggag gccaaaaaaa tcttcagccg catcctcacc tacatggttg
     4861 ccgcctgcct gaccctcttt ctgctcatga gcctcttcat cgatgagctg ctccgtctgc
     4921 gcgtgggggg atattccttc ttcggcgagc ccttctgggc gagcgcccgg gtggtgccgc
     4981 cgatcatgct ggcctcgctc ttttacgccc tctacctggt tttcgaggcg gccctctatc
     5041 tcgagaaaaa gagcggcctc atcgccctgg tgacgatcgc cggagtggga gtgagcctgg
     5101 ccctcaatct cctgctcatc ccgcagtggg ggcccctggg ctcggcctgg gcgcgggtga
     5161 tcgcctacgc ctgcatggcc atggggctgt ttgtctttgc gcagcgatgt tatcccgtcc
     5221 cgtgggaatg gtcccgcctg ctgcagctct gtctgagcgc ggccctcatc tatgccctgg
     5281 gaaaactgcc cctgctgcat ccagcggtgg cggtccgcct ggcccttttc gccgcctggc
     5341 cgcttttgct cgcggccgcc ggctttttcc gggctgagga gcgcgaaatt atacggggtt
     5401 ttttccgcaa aattatgtaa atttgagcgc
//
LOCUS       DAOPWU010000234         5415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15102_length_5415_cov_4.281716, whole genome shotgun sequence.
ACCESSION   DAOPWU010000234 DAOPWU010000000
VERSION     DAOPWU010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15102_length_5415_cov_4.281716"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKM23_11105"
     CDS             complement(<1..622)
                     /locus_tag="PKM23_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HNY92061.1"
                     /translation="MLIQTADIVVILIYALFMGYLGYYAKRRVQNVEDYFAGGRKVPW
                     WMAAISHHMSGYSAFAFVGHATIAYMSGFSIWTFFAVPIFIAMIIGARVWAPKWVRLK
                     VITPVEYLEERFNLGTRQLFAWSGIGIKFVDEGVKLYSLSVIVHVVTGFSLELVIVAC
                     GLVTMFYLLFGGLWATMLTDFAQFIIQFSVTLLLVPLVLNAVGGWHS"
     gene            complement(672..2342)
                     /locus_tag="PKM23_11110"
     CDS             complement(672..2342)
                     /locus_tag="PKM23_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNY92062.1"
                     /translation="MYKYLLRLVIILLLCSASGRASDRSGRDPLIIFHLDFNSVSLRE
                     EYIRHWLKNAAEMGYNAVLWEIEDEVKWQICPECASPDAFSKEQFRELLEYSRGLGLE
                     PIPLLQTIGHAEYVLRHVPYQTMRENPGRYDCYCTSNPRVRHFLKSWIREYLELFGPV
                     RYFHLGGDEAYAFGSCPLCSLAVTRQGANGLYSEYIRDIAESLIRNGIRPGVWSDMVL
                     KHPATLSLIPKDFIFWDWNYWDGEGSPEEVMVWSMGRRVSGKEITAEVSREFPGIIDS
                     TGALRAFYTSDRLKSLGYEVILCSSSRSHGDGVFAGRHAVHAPNIVAAARKCVQADLL
                     GTCITSWAVRISNYETQQPWFHLAPLTCKNPHLPLRELLRLTGQALFGADSSAFFTAI
                     TGIGQPFPFGSEKTTGIMWTGMKDGRPAPPGHLRELVRAWRSAGDGARWQQTKGDIAM
                     AGENIQTGLDLLNRMILSTQGGSSIMNAWSRAGYFQLWQLYLADELVRHAEGAPLQSN
                     HEMAELAGSLRREYRAWAEEWMTPASADQNAGLIYDAIIDFFTAGGGN"
     gene            complement(2449..3618)
                     /locus_tag="PKM23_11115"
     CDS             complement(2449..3618)
                     /locus_tag="PKM23_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNY92063.1"
                     /translation="MRDAPITKKEIAATTALSFAKVNSITMELQSIGLISETGKTDSN
                     GGRPSALYQIYPDFAFAIGCELSHKKVSTIVVDLTGKIISRHSASFEIKEGKEALIAK
                     ILAGIELELEKSGFPREKFIGIGIAVAGLVNPQDGTSTPFPHLADWGNIALKEIIEKE
                     FDLYCYVENVANAAALAELNYGFNRKIDNILALNVGSGLGMGIILDGQLYKGVTGSAG
                     EFGHITVDENGPLCKCGNVGCLETLASTSAVVAKAKEMIARQVVSRLTSCCDGDPDRL
                     DFKSISEAALQGDKLAYSLLDEMGRNLGEGIVTLINLFNPGMILIGGEVCQAEELILQ
                     PILNVVQKRALEIPRKVAQIVFSRLGGHAGIIGATVPVIEYYFSILPKRVAEMTR"
     gene            complement(3911..4939)
                     /locus_tag="PKM23_11120"
     CDS             complement(3911..4939)
                     /locus_tag="PKM23_11120"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY92064.1"
                     /translation="MRNQIKNTWTLIVMLTLAVNTVIHAGDLKKVAQSGMQYLKIGLD
                     AQMVGRGEAGISSVKGLSALYWNPAGLADMRKKELSFSHNAWIADISLDALAIGYNMG
                     HLGAFSIDAVWVDYGELLGTSVGHSIEESSAQGYVDEGTFRPIDLAVGIGYARKVSNQ
                     FSIGGHVRYLYEDYGSNVIVTTSGENETVQNTIGTLSFSLGTWYQTGFKSLALSMTIQ
                     NFSPDIRYEYESFSPPLTFKMGVSMDLLDFFNDKSRSRLLFAIDAIHPRDYTERINVG
                     MEYDLLGILQLRSGYRMNYDEGELAFGGGVRYSMSDDTQLRLDMSYLTMASGRFDWPM
                     QITAGLSF"
     gene            complement(4936..>5415)
                     /locus_tag="PKM23_11125"
     CDS             complement(4936..>5415)
                     /locus_tag="PKM23_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY92065.1"
                     /translation="DDESLIVGFNYYYAVTAFDDGTQNFLHPGLSLESSRLTSTAYLG
                     ASSALKPEATAKGVSEHLRVVPNPYNIRSRNYGDPNDAQSTENNKILFVGLPAKCTIR
                     IYTVAGDLVKVIEHTNGLGSESWDLISDSHQFIVSGVYVARVESDLNAEIIKFVVIR"
BASE COUNT         1265 a         1545 c         1427 g         1178 t
ORIGIN      
        1 ttgaatgcca gccccccacc gcgttcagga ccagcggcac cagcaggagc gtcaccgaaa
       61 actggattat gaactgcgcg aaatcggtca gcatggtggc ccacaagccg ccaaacaaca
      121 gatagaacat ggtgactagt ccacaggcga cgatcaccag ctcaagagaa aagcctgtga
      181 cgacgtgtac gatcacagag agcgaataca gctttactcc ttcatcgaca aatttgattc
      241 cgatgccgct ccaggcgaag agctgccgcg tgcccagatt aaaccgctcc tcgaggtatt
      301 caacgggtgt aatgaccttc aggcggaccc atttcggcgc ccacactctg gcgccgatga
      361 tcatcgcgat aaaaataggc acggcaaaaa aggtccagat tgaaaatccg ctcatatagg
      421 cgatcgtcgc atgcccgacg aaggcaaagg cactgtagcc ggacatgtgg tgagagatgg
      481 ccgccatcca ccagggtact ttacggccgc cggcaaaata gtcttcgaca ttttgtacgc
      541 gccttttggc ataatatccc aagtacccca tgaacagggc atagatcagg ataaccacaa
      601 tgtccgctgt ctgaatgagc atcgaatagc cctttagtta gagtgcgacg aaacgaaccg
      661 gcgaagcgga actagttccc gccaccggcc gtgaaaaagt caatgatggc atcgtagatc
      721 agccctgcat tctgatcggc ggaggccggc gtcatccact cttccgccca tgctctgtat
      781 tcccgccgca gcgagcccgc caattcggcc atctcgtggt tcgactgcaa aggagcacct
      841 tcggcatgcc ggaccagttc atcggcaagg tacagctgcc ataattggaa atagcccgcc
      901 ctggaccagg cattcattat ggacgatccc ccttgtgtgg acagtatcat ccggttcagg
      961 agatccagcc ccgtctggat attttctcca gccatggcaa tatctccttt cgtttgctgc
     1021 catcgggccc cgtcaccagc cgatcgccag gcgcggacga gttcccgaag gtgtcccggc
     1081 ggagccggac ggccgtcctt cataccggtc cacatgatac cggtcgtctt ttcagagcca
     1141 aagggaaagg gctgcccgat gccggtgatc gctgtgaaaa aagcactgct gtccgccccg
     1201 aacagcgcct gcccggtaag acgcaggagt tcgcgaagcg gtaggtgcgg atttttgcag
     1261 gtcaggggtg caagatgaaa ccagggctgc tgggtttcgt aattggagat gcgcaccgcc
     1321 cagctggtga tacaggtgcc cagcagatcc gcctggacac attttcgcgc cgctgccacg
     1381 atgttggggg catgcaccgc atgcctcccg gcaaaaacgc catccccgtg ggaacgcgag
     1441 gagctgcaga ggataacctc gtatccgaga ctcttcagcc tgtcagaggt gtaaaaagcc
     1501 cgcagggcgc ctgtactgtc gatgatgccc gggaactccc ggctcacctc cgcggtaatt
     1561 tcctttccgg acaccctgcg ccccatgctc cagaccatga cctcttcggg ggagccttcg
     1621 ccgtcccagt aattccagtc ccagaagatg aaatctttcg gaatcagcga taaagtggcg
     1681 ggatgtttca ggaccatatc gctccagacc cccggccgga tgccgttccg gatcagggat
     1741 tccgcaatat ccctgatgta ttcgctatag agcccattcg cgccttgccg ggtaaccgca
     1801 agggagcaga ggggacagct gccgaaggca taggcttcat cgccgccgag atggaaatag
     1861 cgcacaggac cgaacaattc gagatattcc cggatccagc tctttaaaaa atgccggacg
     1921 cgcggattgc tcgtgcagta gcagtcgtac cgtcctggat tttcccgcat ggtttggtac
     1981 ggaacatgtc gcaggacata ttcggcatgt ccgatggtct gcaagagcgg gatgggttcc
     2041 agccccagtc cgcgcgaata ttccaggagt tccctaaact gctcctttga aaatgcgtcc
     2101 ggactggcac actccgggca gatctgccac ttgacctcat cctcgatctc ccacagcacg
     2161 gcattgtagc ccatctccgc cgcattcttg agccaatgcc ggatatattc ttcgcgcagg
     2221 gaaacggaat tgaaatcgag atgaaaaatg atcaggggat cgcgaccgct ccggtccgaa
     2281 gcccgtccgg acgcactgca caacaggagg ataataacta atcgaaggag atatttatac
     2341 atgttttctc tatccgcaaa ttcattcgtc tcttacagga agtactgaac cgcatcacca
     2401 ggctctcccg cacaccttgc gagatagaga ggccctgatc gtgggccgct accgggtcat
     2461 ctcagcgacg cgcttcggca agatggaaaa ataatattcg atcaccggca cggtagcgcc
     2521 gatgatgccg gcatggccgc ccagtcgcga aaacacgatc tgggccacct ttctcgggat
     2581 ctccagcgcg cgcttctgga cgacattcaa gatcggctgc aggatcagct cctcggcttg
     2641 acagacctcc ccgccaatga ggatcatgcc cggattgaag agattgatca gggtgacaat
     2701 tccctccccc agatttctgc ccatctcatc gaggagagaa taggcgagtt tgtcgccctg
     2761 cagggccgcc tcgctgatcg atttgaaatc gaggcgatcc ggatcgccat cgcagcagct
     2821 ggtcaggcgc gatacaacct gtctggcgat catctctttg gcttttgcga cgaccgcgct
     2881 ggtgctggca agggtttcaa ggcagccgac gttgccacac ttgcacagcg gaccattctc
     2941 atccaccgta atatggccga attcgcccgc actcccggta acccctttgt acaattgccc
     3001 gtcgagaatg atgcccatac ctaatccact gccgacattc aaggcgagga tgttgtcgat
     3061 cttgcggttg aagccatagt ttaattcggc gagcgccgcg gcatttgcga cgttttcaac
     3121 atagcagtag agatcgaatt ccttctcgat gatctccttg agggcaatat tgccccagtc
     3181 ggcgagatgg ggaaaggggg tcgaggtgcc atcctgcgga ttcaccaggc cggccaccgc
     3241 gatgccgatg ccgataaatt tctcgcgcgg gaatcccgat ttttccagct ccagttcgat
     3301 gcccgccaga atcttggcga tcaacgcctc tttcccctcc ttgatctcaa aactcgcaga
     3361 gtggcgcgaa atgatcttgc cggtcagatc aacgacgatg gtactcacct ttttgtgcga
     3421 caattcgcag ccgatggcaa acgcaaagtc gggatagatc tgatagagcg cggagggcct
     3481 tccgccgttg gaatcagttt taccggtttc ggagatcagc ccgatggact gcagttccat
     3541 ggtgatcgag ttgactttgg cgaaactcaa tgcggtggtt gcggcaatct cctttttcgt
     3601 gatcggtgca tcccgcatca atttccggat gacgatatcg atattgtgct ctcgcaaccg
     3661 ggtttgactg ccaccgtttg ctttgtcttt caacattttc atagacgcaa atactctcca
     3721 ttttcgatgc aaactgcggc aagggcccta aaccggttcc ggtatcatca tcctagggag
     3781 ggtgccgatg aatacttatt gcaaaagcaa cttgaaaaaa aagtggcttt accgggcaat
     3841 ctgctcatct ctccccatcc gggactaaag tccgcctgcg ttcagtgact ctgcacgcat
     3901 cgatccccca tcagaaactc aaaccagcgg tgatctgcat gggccagtcg aaccggcccg
     3961 atgccatcgt caaatagctc atatccagcc tgagttgggt atcatcggac atggaatagc
     4021 ggacgcctcc gccgaaggca agttcgccct catcatagtt cattcggtaa ccgctgcgca
     4081 gctgaaggat gcccagaagg tcatattcca tcccgacatt gatcctctcg gtgtagtcgc
     4141 ggggatgtat ggcatcgatg gcgaagagca agcggctgcg ggacttgtca ttgaaaaagt
     4201 caagcagatc catggaaacg cccatcttga aggtcagggg aggactgaag gactcgtact
     4261 catatctgat gtctggcgaa aagttctgaa tcgtcatcga cagcgccaga ctcttgaaac
     4321 ccgtctgata ccaggtgccc aggctgaagc tgagcgtgcc gatcgtattc tgcaccgtct
     4381 cattttcgcc gctggtggtg acgatgacat tgctgccata gtcttcataa agatagcgga
     4441 cgtggccccc gatgctgaac tgattggata ctttgcgcgc atagccaatc cccacggcca
     4501 ggtcgatggg tctgaaagtc ccttcatcga catacccctg cgcggaagac tcttcgatcg
     4561 aatggccaac cgaagtgccc agcagttcac cgtaatcgac ccaaacggca tcgatggaaa
     4621 acgcgccgag gtggcccata ttataaccaa tggcgagggc atccagggaa atatcggcta
     4681 tccaggcatt gtgactgaag gagagctctt tcttgcgcat atccgcaagt ccggccgggt
     4741 tccagtataa cgcggagagt cccttgacag aagagatgcc ggcctcaccc cttccaacca
     4801 tttgcgcgtc cagtccgatt ttcaggtact gcatgccact ctgggcgacc tttttcaaat
     4861 cgcccgcatg aatgaccgtg ttaaccgcaa gggtcagcat gactatcagc gtccaggtgt
     4921 tcttgatctg atttctcatc gtattaccac gaatttaatt atttccgcat tcaagtcgga
     4981 ctcgacacgg gcaacataca ctccactgac aatgaattga tgtgaatcgg aaatcaaatc
     5041 ccatgattcg gagcccagtc cattagtatg ttctatgacc ttgaccagat ccccggccac
     5101 ggtatagatg cgaatcgtgc atttagccgg caaccccaca aagagaatct tgttattctc
     5161 ggtgctttgg gcatcattgg gatcgccata gttgcgagaa cggatattgt agggattggg
     5221 tacgacgcgc aaatgctcgg aaacgccttt cgcagtggcc tccggtttga gcgcgcttga
     5281 ggcgcccagg taggcggttg aggtcaatct cgaactctcg agtgagagac ccgggtgcag
     5341 aaaattctgc gtaccgtcat caaaggcggt gaccgcgtaa tagtaattaa aaccgacgat
     5401 caggctttca tcatc
//
LOCUS       DAOPWU010000235         5401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15165_length_5401_cov_5.211186, whole genome shotgun sequence.
ACCESSION   DAOPWU010000235 DAOPWU010000000
VERSION     DAOPWU010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15165_length_5401_cov_5.211186"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PKM23_11130"
     CDS             complement(<1..482)
                     /locus_tag="PKM23_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY92066.1"
                     /translation="MTAAGVKEKAGALRVCIFTSVHRPYDVRVFHRQARSLTEAGHEV
                     TLLVHAPFARERRDGVEVIGLKPPSGRLMRLCSSWRFLRAALSQHAEVYHFHDLELLP
                     AGLLLRIWSRRAVIYDCHENYPEAILERAWLPAWLKPVLSRLVGWFEPALARHLDAVI
                     "
     gene            complement(482..2632)
                     /locus_tag="PKM23_11135"
     CDS             complement(482..2632)
                     /locus_tag="PKM23_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNY92067.1"
                     /translation="MYTAIAEELNLGWSQVKSTIELLDADNTVPFIARYRKEATGSLD
                     EEQIRAIQERIRYLRNLEERRQAILKSIEEQGKLTPELEAALQAADRLQTLEDLYLPY
                     KPKRRTRATMAREKGLEPLAQLILAQEILSGEPLEYAHSFVDPEKGVEDAEAALAGAR
                     DICAETISDDARVRQTVRERTLARGFLTSEARDPDNCKEYEIYKQFSEPVKTIPPHRI
                     LAINRGERENFLRAAIEAPEAELVLEIEKLYVTQPRSIFTGELKKAAADAYHRLIAPA
                     IEREIRNQLTERADEHAIRIFAQNLRALLMQPPLRGKVILGIDPGFRTGCKIAAIDRT
                     GKYLEGGTIYPHEPQRRWREAVETISEFIADYQIDIIAIGNGTASRETEKLAAEVIAE
                     CGRPVQYVIVSEAGASVYSASPVARKEFPDLEASLRGNISIARRLLDPLSELVKIDPK
                     SIGVGLYQHDVDQIHLAESLDQVVESCVNSVGVDLNTASVSLLQYVSGVNGRVAENIV
                     RCREERGKFTTREELKAVKGLGENAFVQAAGFLRLPESERFFDRTAVHPESYAAAERL
                     LQELGLAVAQVQENGRLVSQRLKQSGRTLTELAGVCGCGAETLQDIIASLEKPNRDPR
                     DEMPKPILRSDVLSIEDLSEGMVLKGTVRNVVDFGAFVDIGVKEDGLVHLSRMAKKFV
                     KHPLDIVKVGDVIEVKVITIDRERHRIGLSMVLD"
     gene            3054..3494
                     /locus_tag="PKM23_11140"
     CDS             3054..3494
                     /locus_tag="PKM23_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNY92068.1"
                     /translation="MAESGFDRDSFFKGLLIGGTLGALAALLFAPKSGKELRGDIVRK
                     SEEAVEGSKRIYHDARSRASAIITEAVEKAQALREEASEQLATARMKAEELLTTANQK
                     VEELGESAKGYIHDTREEVAKKKVKAEKADVAGGDAARKAFGKK"
     gene            3522..3917
                     /locus_tag="PKM23_11145"
     CDS             3522..3917
                     /locus_tag="PKM23_11145"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HNY92069.1"
                     /translation="MLEYSVVVIAAVMVLTWIALIVTLFYVIRLIKSVQRLTEEARPH
                     IAPISHDITIITQKTARILESVQRQTFMIEESVDNVRQISRNVKDFEANLLAKIALPA
                     VKLSQLGQGIKRGFDVVYNRFTHHRKRSL"
     gene            4078..>5401
                     /locus_tag="PKM23_11150"
     CDS             4078..>5401
                     /locus_tag="PKM23_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HNY92070.1"
                     /translation="MKRMLLFLLLIAATAIAGAADSGTLLLRFPAIHGDQIVFSYAGN
                     LYTVSAQGGTARRLTSHDGYEMFARFSPDGRQIAFTGQYDGNTEVFLIPAQGGVPERL
                     TWTATLDRDEVSDRMGPNTIVLGWTPDGKEILFRSRMREFNDFKGQLYLVPAGGGMPE
                     QLPLPRGGFSSFSPDGSRLAYNRIFREFRTWKRYRGGMADDIWIYDRNSKETRNLTNN
                     PASDIFPMWSGDRIYFLSDRDENKRMNLFVCTPEGGETRQLTFFTDYDIKFPSLGDQA
                     IVFEKGGAIYRFDLASGTASAVPVTIAEDLAASRSGLLDVSPFIANFEISPDGQRALF
                     GARGDLFTIPAKYGEIRNLTATPGIHERSSTWSPDGRWIAAISDRSGEDEIWLFSQEG
                     KSAPRQITSSGDVYKYRPIWSPDSRKLLWADKKLRLQYVDIESQKITLV"
BASE COUNT         1085 a         1769 c         1500 g         1047 t
ORIGIN      
        1 cagatcaccg catccagatg gcgggccaga gccggttcaa accagcccac caggcgcgag
       61 agcacaggct tcagccatgc gggcagccag gcgcgctcaa ggatcgcctc tggatagttc
      121 tcatgacaat cgtaaatgac ggccctgcgg ctccagatcc tgagcagcag tccggccggc
      181 agcagctcca gatcgtggaa atggtagacc tccgcatgct ggctcagagc agcgcgcaaa
      241 aaacgccagg aagagcaaag ccgcatcagc cggccggaag gcggcttcaa gccgatgacc
      301 tccactccgt cacgccgttc acgggcgaac ggggcgtgga ccagcagggt gacctcatgg
      361 cccgcctcgg tcaggctgcg cgcctgacga tgaaataccc gcacatcata ggggcggtgt
      421 acactggtga aaatacagac ccgcagcgct ccggctttct cttttacccc tgcggccgtc
      481 atcaatccag caccatgctc agaccgatgc gatggcgctc ccggtcgatg gtgatcacct
      541 tgacctcgat cacatccccg accttgacga tatccagggg atgcttgacg aactttttcg
      601 ccatgcggct gagatggaca aggccgtcct ccttgactcc gatgtcgaca aaggctccaa
      661 agtcgacaac attgcgcacg gttcccttga gcaccatccc ctcgctcaag tcctcgatgc
      721 tgagcacatc gctgcgcaag atcggcttgg gcatttcatc acgtgggtcg cggttgggtt
      781 tttccaggct ggcgatgata tcctgcaggg tctcggcgcc gcagccgcac accccggcca
      841 gctcggtcag ggtgcgcccg ctctgcttga gccgctggct caccagccga ccgttctcct
      901 gcacctgcgc cacagcgagg ccgagttcct gcagcagccg ctccgcagcc gcgtaggatt
      961 cgggatgaac cgccgtccgg tcgaaaaagc gctcgctctc gggaaggcgc agaaaacccg
     1021 ccgcctgcac aaaggcattc tcgcccagcc ccttgaccgc cttgagttcc tcgcgggttg
     1081 tgaattttcc cctctcctcg cggcagcgga cgatattctc cgccaccctg ccgttgacac
     1141 cggagacata ttgcagcaga gaaaccgagg cggtattcaa atcgaccccc acactgttga
     1201 cgcacgactc caccacctgg tcgagggatt cggccagatg gatctgatcg acatcgtgct
     1261 gataaagccc gacgccgatc gacttgggat cgatcttgac cagctcggaa agcggatcaa
     1321 gaagacggcg ggcgatggag atgttgccgc gcagcgaggc ctccagatcc ggaaactcct
     1381 ttcgcgctac cggcgaggcg gaatagaccg aggccccggc ctcgctgacg atgacatact
     1441 gaaccggccg gccgcattcg gcgatcacct cggcggccag cttttcggtc tcccggctgg
     1501 cagtgccgtt gccaatagcg atgatgtcaa tctgatagtc cgcaatgaat tcgctgatgg
     1561 tctccaccgc ctcccgccag cgccgctgcg gttcatgggg atagatggtg ccgccctcga
     1621 ggtattttcc cgtccggtcg atggcggcga tcttgcagcc ggtgcgaaaa ccgggatcga
     1681 tgccgagaat gaccttgccg cgcagggggg gctgcatcag aagcgcgcgc agattctggg
     1741 cgaagatgcg gatagcatgt tcatccgccc gctccgtgag ctggttgcga atctcccgtt
     1801 cgatcgccgg ggcgatgagg cgatgatagg catcggcggc ggctttcttc agctcgccgg
     1861 tgaagatgct gcgcggctgg gtgacataga gtttttcgat ctccagcacc agctccgcct
     1921 ccggcgcctc gatggctgcg cgcaaaaagt tctcccgctc gccccggttg atggccagga
     1981 tgcggtgggg cggtatggtc ttgaccggtt cggagaactg cttgtagatc tcgtactcct
     2041 tgcagttgtc gggatccctg gcctccgagg tgagaaatcc ccgcgccagg gtgcgctcgc
     2101 gcaccgtttg ccggacccgg gcatcatcgg aaatggtttc cgcgcagata tcgcgcgccc
     2161 ccgccagggc cgcctccgca tcctccactc ccttttcagg atccacaaag gagtgggcat
     2221 actcgagggg ctccccgctg agaatctcct gggcgagaat gagctgcgcc agcggctcca
     2281 gccccttctc tcgcgccatc gtcgcccggg tgcgccgctt cggcttgtag ggcagataaa
     2341 ggtcctccag ggtctgcagt ctgtcggccg cctgcagggc cgcctccagc tcgggcgtca
     2401 gcttgccctg ctcctcgatg ctcttgagga tggcctggcg ccgctcctca agattgcgca
     2461 aatacctgat ccgctcctgg atggcgcgta tctgctcctc atccagactc cccgtcgcct
     2521 ccttgcgata acgcgctatg aaggggacgg tattgtccgc atccagcaac tctattgtgc
     2581 tcttgacctg gctccagccc aggttcaact cttccgcaat ggcggtgtac atctgctgct
     2641 cttgcacgcc ggtattcctg atgttggttc gggatttgtt ggatagtgtg taaatatatc
     2701 ccgcgcaggg gagaaaagca agttttttac ctgttgcacg gccaatccct ccccttgtgg
     2761 aatctaatac cggccaggct ggttatagta agagcctgtc cgctcaaaaa tggtatgata
     2821 gcccccggaa tgagagtccg cggcgccagg ggtccggtgg gagccagctt tcgggggtcg
     2881 atcagaccaa aaatgggcct tgactttttg ataaattttt gctaattgta gtattaccgg
     2941 tgtgatccag cggtcatcaa gaccggaccg cacaaaccaa cccgcaattc ccaaccgtct
     3001 atatcatatt gcggccaaaa ttatctcatc atctctatca ggaggaaatt accatggctg
     3061 agagtggatt cgatcgcgac agttttttca agggcctgct catcggcgga accctgggcg
     3121 ccctggctgc cctgcttttc gcccccaagt caggtaaaga gttgcgcggc gatatcgtcc
     3181 gcaagagcga agaggcggtc gagggctcca aacgcatcta tcacgatgcc cgctcccgcg
     3241 cctccgccat catcaccgaa gcggtcgaga aggcccaggc cctgcgcgaa gaggccagtg
     3301 aacagctcgc caccgcccgc atgaaggccg aagagctcct cactaccgcc aaccagaagg
     3361 ttgaagagct gggcgaatcc gccaagggct atattcacga caccagggaa gaggtcgcaa
     3421 aaaaaaaagt gaaggctgaa aaagcggatg tcgcaggggg cgatgccgcg cgcaaggcgt
     3481 ttggcaagaa ataaccccaa ggcggaagga gtgcccctgt catgctagag tacagcgtgg
     3541 tcgtcatcgc cgccgtcatg gtcctgacct ggatcgcctt gatcgtgacg ctcttttacg
     3601 tcatccgcct gatcaaatcg gttcagcgcc tgactgagga agcacggccc catatcgcac
     3661 caatcagcca cgatatcacg atcatcaccc agaaaacagc gcgcatcctt gaatcggtcc
     3721 agcgccagac cttcatgatt gaggagagtg tggataacgt ccgccagatc tcccgcaacg
     3781 tgaaggactt tgaggcaaac ctgctcgcca agattgccct gcccgcagtc aagctctccc
     3841 agctcggtca aggcatcaag cgcggcttcg atgtggtcta caaccgcttc acccatcata
     3901 gaaagagatc tctttgagat cccgtcgcgg tcaaagccct gctgccttaa cacgccgcag
     3961 ggcttttcca ttgtgccctg cggcaagctg ccgactcgcg accaggagcg ttgcgccctc
     4021 cacccctttt ctctccattc agcttttgga tccccacgaa caagacaagg aaataccatg
     4081 aaacgaatgc tcctcttcct gctcctgatc gcagcgaccg caatcgccgg cgctgcggac
     4141 tcgggcactc ttctgctccg cttccccgcc atccacggcg atcaaattgt cttcagctat
     4201 gccggcaacc tctacaccgt ctccgcacag gggggcaccg cacgccgcct gaccagccac
     4261 gacggctatg aaatgttcgc ccgcttctcc cccgacggcc ggcagatcgc ctttacggga
     4321 caatatgacg gcaacaccga agtctttttg atccccgccc agggcggcgt tcctgagcgc
     4381 ctgacctgga ccgccactct cgatcgtgat gaggtctccg accggatggg gcccaatacc
     4441 atcgtcctgg gctggacacc cgacggcaag gagatcctct tccgctcccg catgcgcgag
     4501 ttcaacgact ttaaaggaca gctctatctg gtcccggccg gcggcggcat gccggagcag
     4561 ctccccctac cacgcggggg cttctcctcc ttctcgcccg acggcagcag gctcgcctac
     4621 aaccgcatct tccgcgaatt caggacttgg aaacgctacc gcggcggcat ggccgatgat
     4681 atctggatct acgaccgcaa cagcaaggag acccgcaacc tcaccaacaa tccggcttcc
     4741 gacatcttcc cgatgtggtc aggagaccgg atctattttc tctccgaccg ggatgaaaac
     4801 aaacggatga acctcttcgt ctgtactccc gagggtggag agacgcgcca gctcaccttt
     4861 ttcaccgatt atgacataaa atttccctcc cttggcgacc aggcgattgt cttcgaaaag
     4921 gggggcgcca tctaccgatt tgatctcgcc tccggcaccg cctccgccgt cccggtcacc
     4981 atcgccgagg acctcgccgc cagccgctcc gggcttctcg atgtcagccc cttcattgcc
     5041 aacttcgaaa tctccccgga cggccagcgc gccctcttcg gcgcccgcgg cgacctcttc
     5101 accatcccgg ccaaatacgg cgagatccgc aacctcaccg ccacacccgg cattcacgaa
     5161 cgcagcagca cctggtcgcc ggacggacgc tggatcgctg ccatctccga tcgcagcggc
     5221 gaagacgaaa tctggctctt cagccaggag ggcaagagcg ctccccgcca gattaccagc
     5281 agtggcgacg tttataaata ccggcccatc tggtcgccgg acagccgaaa actgctctgg
     5341 gccgataaaa agctgcgcct gcagtatgtc gacatcgaga gccaaaagat caccctcgtt
     5401 g
//
LOCUS       DAOPWU010000236         5399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15173_length_5399_cov_5.925524, whole genome shotgun sequence.
ACCESSION   DAOPWU010000236 DAOPWU010000000
VERSION     DAOPWU010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15173_length_5399_cov_5.925524"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2704
                     /locus_tag="PKM23_11155"
     CDS             <1..2704
                     /locus_tag="PKM23_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNY92071.1"
                     /translation="ASSYEWDNFNYTPPVVDVGALPRITFKARASLDCELVFKPIYGD
                     GSDNWLVLQLPGDNSWHDYAFDLTPLTNTLLTRIYLYLDGGTTQPKAGVVYFDDLFIG
                     DAAGAAAADGSALAAALSAAVLLYDGAVEGAGEGEYPAGARSSLQMAIAVAQAVAGRL
                     DASQSEIDQAEWDLLDACVNLEKRANIPNPGLIDPLATPRTKYLYMNLEQGCGQRLLF
                     GMHDATGYGVGWKGDDDRSDVRDVCGDYPALYSWDMNAVDRGSPQEMARFTYRIKSAF
                     ARGGVNTLCWHQYDPLGRSFYGSKVSDNVVATLLPGGAWHDFYKKRLARIARFLKGLR
                     GEDGHSIPIIFRPYHEHDSGAFWWGTNNCTTEEYNLIWQFTVTYLRDVLNVHNLIYAI
                     SPISFTTRNSYLKIYPGDDYVDILGMDFYYWYPLADRQKSFINSLHIPAQLALERGKV
                     PALTEVGLDTLLEPSWFNRYLLPPFKSDSLAMRMAYTAVWRNESVHHHFAPYPGHPSV
                     PGFLEFYNDPYTAFERDLPPMYIHPGSDQTPPSFTITPDTFFIATDTLFPLYFETDER
                     AHLGFAFSDIPYNLMSGAFLSGQGGFKHSTLLALQQGEERTFFIRAIDISGNETPEAF
                     RVTVRVDTLQRRIPWYDDRYPAGGWKSGAAPLGYGAAAGNATTTAAVKSLYFVTNFNL
                     PHPVTQLGLLLKCHDGAVVRINGRELLRYNLPEGEVTPDTPAASAVKTNQIYVFTAED
                     LQLLKNGENLLSVELHAAGLAGADLSLDARLFDATGIYLNLGATWHYFDGGEPPAFTL
                     ADYLSDVPPPPASLPRAFGLAQNYPNPFNPETAIRFHLTRPGEIRLEVYDITGRMVVR
                     LIDGRREAGEHGVVFNGAGLPSGIYLYRLTAEGKHQVKRMLLLR"
     gene            complement(2776..3012)
                     /locus_tag="PKM23_11160"
     CDS             complement(2776..3012)
                     /locus_tag="PKM23_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92072.1"
                     /translation="MEEREKQIPIWFFIGAIVLIYGALITATGFLGWVSLPPAGTRVA
                     LWELHADLWWGLLLLAIGLFYSLKYRPRRKHRNA"
     gene            complement(3683..5131)
                     /locus_tag="PKM23_11165"
                     /pseudo
     CDS             complement(3683..5131)
                     /locus_tag="PKM23_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786299.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system.; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
BASE COUNT         1124 a         1633 c         1628 g         1014 t
ORIGIN      
        1 ggcttcttcc tacgaatggg acaatttcaa ctataccccg cctgttgtgg atgtcggcgc
       61 cttgccccgc atcaccttca aggcgcgcgc cagcctcgac tgcgaactgg tcttcaagcc
      121 gatctacggc gatggcagcg acaactggct ggtcctgcag ctgccggggg acaacagctg
      181 gcatgattat gcctttgact tgactccgct caccaacacc ctgctcacaa ggatctatct
      241 ctacctcgac ggcggcacga cccagcccaa agcgggcgtg gtctatttcg acgacctttt
      301 tatcggggat gcagcgggtg ccgctgctgc cgatggttca gccctcgccg cggccctctc
      361 cgccgcggtc ctcctttatg atggcgctgt cgagggcgcg ggcgaaggcg agtaccccgc
      421 tggcgccaga agctcgctgc agatggccat cgccgttgca caggctgtcg ccggcaggct
      481 ggatgcgagc cagtccgaaa tcgatcaggc cgaatgggat ctcctcgacg cctgcgtcaa
      541 cctcgagaaa cgggccaata ttcccaatcc cggcctgatc gaccctctgg ccacgccccg
      601 gaccaaatat ctctacatga atcttgagca gggctgcggc cagaggcttc tcttcggtat
      661 gcacgacgcc accggctatg gtgtcggctg gaagggcgac gacgaccgct ccgacgtccg
      721 cgatgtctgc ggtgattatc ccgccctcta cagctgggac atgaacgctg tcgaccgcgg
      781 ctcgccccag gagatggcgc gctttaccta ccgcatcaaa tctgccttcg cccgcggtgg
      841 ggtcaacacc ctctgctggc accagtacga tcccctgggg cgcagctttt acggcagcaa
      901 ggtcagcgac aacgtcgtcg ccaccctgct tcccggcggt gcctggcacg acttttacaa
      961 aaaacgcctt gcccgcatcg cccgctttct caaggggctg cgtggagagg acggccactc
     1021 catcccgatc atttttcgcc cttaccatga gcacgacagc ggcgccttct ggtggggcac
     1081 caataactgc acaaccgaag agtacaacct gatctggcag ttcactgtca cctacctgcg
     1141 cgatgtgctc aatgtccaca acctgatcta tgcgatctca ccgatcagct tcactacgcg
     1201 caacagctat ctcaagatct atccgggcga cgattatgtc gacatcctgg ggatggattt
     1261 ctactactgg tatcccctgg ccgaccggca gaagagcttc atcaacagcc tgcacatccc
     1321 cgcccagctt gcgctggagc gcggcaaggt gcccgccctc accgaggtcg gcctggatac
     1381 cctgctggag ccctcctggt ttaaccgcta cctgctgccg cccttcaaga gcgacagcct
     1441 ggcgatgaga atggcctata cggcggtctg gcgcaacgag tcggtgcacc accattttgc
     1501 gccctatccg gggcacccca gcgtgccggg atttcttgaa ttctataatg atccctacac
     1561 cgccttcgaa agggatctgc cgccaatgta cattcacccc ggctcggacc agaccccgcc
     1621 cagcttcaca atcacgccgg acaccttttt tatcgctacc gatactctct ttcctcttta
     1681 tttcgaaacc gatgagcgcg cccatctggg ctttgccttt tccgacatcc cctataacct
     1741 catgtccggg gcttttctat ccggccaggg gggattcaag cattccaccc tgctggcgct
     1801 gcagcagggc gaggagcgca ccttttttat ccgcgccatc gacatcagcg gcaacgagac
     1861 ccccgaggcc ttccgggtga ccgtcagagt cgataccctg cagcggcgca ttccctggta
     1921 tgacgatcgt tatccggctg gcggctggaa gagcggcgca gcccccctgg gctacggcgc
     1981 cgcggccggg aacgccacca cgaccgcggc cgtaaaaagc ctttattttg tcacgaattt
     2041 caacctgcct caccctgtga cccagcttgg cctgctgctc aagtgccatg acggtgcggt
     2101 tgtacgcatc aatggccgcg aactgctgcg ctacaatctg cccgaaggag aggtcacccc
     2161 tgacaccccg gctgccagcg ctgtcaagac caatcagatt tatgttttca cggcggagga
     2221 tttgcagttg ttaaagaacg gcgaaaacct gctctcggtg gagctccacg ccgccgggct
     2281 cgccggcgct gacctctccc tcgatgcccg gctcttcgac gcgaccggta tctatctcaa
     2341 ccttggtgca acctggcact attttgacgg gggagaaccc cctgccttca ccctggccga
     2401 ctatctcagc gacgtgccgc caccgcctgc cagtcttccc cgcgctttcg ggctggccca
     2461 gaactacccc aatcctttca atcccgagac cgcaatccgc tttcatctga ccaggccggg
     2521 agaaatacgg ctggaggttt atgatatcac cggcagaatg gtggtgcggc tgatcgacgg
     2581 ccgcagggaa gccggagaac acggcgtggt tttcaacggt gcgggtttgc cgagcgggat
     2641 ctacctctac cgcctcactg cggagggaaa acaccaggtg aaacgcatgc tgctgctgcg
     2701 ctgagcggct gagcagtgaa ttaaaaagcc cggactgaaa aggatcagcc gggctttttt
     2761 atcataggtg agcggtcagg cgttgcggtg cttgcgacgg ggccggtact tgaggctgta
     2821 aaagaggccg atggccaaca gcaagagccc ccaccacaga tcggcatgca gctcccagag
     2881 ggcgacccgc gtcccggccg gtggaaggga gacccagccg agaaatccgg tcgccgtgat
     2941 cagcgctccg tagatcagga cgatcgcccc gatgaagaac cagatcggga tttgtttttc
     3001 tctctcttcc atagtgacct caccagaaaa tgatgttgat gacaatgaaa acagccatga
     3061 cgacggccgc ccagaaaagg ggcttgcggt gccatggtga ggcttcctct ttgggcaggg
     3121 gcgtcagtcc catcaccaga ttcttcagct cggcctcggg cttgggccgg gtgaaaaggc
     3181 tgaccacgat ggtgaccagc aggcagacca ccagcgacca ccaggcgctg tacatgttga
     3241 ccgccatcgg tttggccttg tcggagcggg cgatgacctt gacatggtcg ggattgaagg
     3301 ccgccgggtc gaagcggcgg gagatgaagg tcgaacggac cttgacgccg ggactgatgc
     3361 gtgccgggac ggcggagagg ccgaatttct gctgctcccg ggtatcggca atcaccacct
     3421 cgggggccag gatctggatg ggcgcggcgg tcttgcgcag cggcgcgacc tcgcgcatgt
     3481 agaggggctg atcctgcacg gtgaattccc cgccctcttc atggccgaaa aggggcacat
     3541 tgaccagatc gagggcgccg ctttcgatgc tgatgtagga gatggctccc ggtttagccg
     3601 cattggcccg ggtgaggaga acctcggctc cgtggctgag cgtcaccttg ggctggggct
     3661 gatagccgtg cggaaaggta tggacaaaag cccacatact gattgagaag ataatagcga
     3721 caagaaaacc ccagaatccg ccggcagggg tggctcgctt ccagagcatg ccgaggatca
     3781 ccgtgccgaa caggggcacg atgaagaaaa agaccagcac ctggagatac tcgagaatat
     3841 tggagaagaa aaagagggta taggcagtgg cgatggaaag cagcacgccg atcaccgagc
     3901 tccagcggcc catgttgaga tagtggcggt cgctgccctt tctgttgatg agcggtttgt
     3961 agacgtcgtg ggtccagacc gtggcgaagg cgctgacatt gccggccatc cccgacataa
     4021 agccggcgat catcgctgtc actcccaggc cgagcagccc aggaccgaga tacttgccca
     4081 tcagcagggg gaggacgtca ttgaaggtgt tgtgggtgat gccggcgcgc ggatcgcttt
     4141 cgggcaccag caccatcggc gagccgtccg aattcatcag taccgccagc ccgagcaggc
     4201 cggggatggt gacgatcagg ggcaccatca tcttcagggc cgccccgatg atggtgccgt
     4261 tctgggccga gcgcaggtcc ttggcgacaa tgacgcgctg cacctggagg aaatcggtgc
     4321 accagtaacc aaaggcgacg gcgatcccct ggccgaagag catgccgaac cagtcgatcc
     4381 ccatcgggtt gctggcaaag gagccgaggt tctgccacag actggtgaaa tcggcgtttt
     4441 gcaccgccgg gtggatcacc gccacattgc gctgcaccgc cgccatcatt ttgccccagc
     4501 cgcccgcgtg gatgatgccg aggatgggga tgagcagtga gcccagccag atcaggaaga
     4561 actgcagcac ttcgttaaag accgccgaga gcagtccgcc cagggtgacg tagacagcca
     4621 cggtgagaga agccacccag atgctgatat tcatgtccca gccaaggagg agattgagga
     4681 tcttggccat ggcgaacatg ctggcgccgc tcaccgccac ggtcatgaat gaaaaggaga
     4741 tgccggcgac ggagcgcgcg ccctcaccgt agcgcagctg cagatagccc ggcaccgagt
     4801 gggtgcgtga gatgtagtaa aagggcatca tcacaatggc gaggaagagg atagccggga
     4861 tggcgccgat gaggtagccg tgggcgccga gcatgccgta ctggtaggcc atggcactcc
     4921 agcccatggt ctccagggag ctgaggttgg cggagatgaa gctcagcccg gcgatccagg
     4981 cggtcatctt gcggccggcc atgaaaaagt cgtcgccggt gctggcgaac tttttgagat
     5041 aaaagccgat ggcgaggacg aagaaaaaat agatgatgat gatgagcaga tcgaccggcc
     5101 ggaggtgaag catgctcgcg gagccggaca tagcggcgat cagggagaga aaggccatag
     5161 gcgctctttc ggttgatggg ttgcagcctg cacaggaagg gcatcgcact ctgcccccgg
     5221 aagctggagg tggagcggtt gacgtgctgc aaatgtaacc aatggaggag acagataaaa
     5281 ggattttttt tatcccgtct gctgtaaaat tgcttgttaa aaagcgactc cgaccctata
     5341 ttgcgggaga ggaatttgat gcgctgataa ccgtagatac gggagaccag aacatgccc
//
LOCUS       DAOPWU010000237         5376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15277_length_5376_cov_3.541064, whole genome shotgun sequence.
ACCESSION   DAOPWU010000237 DAOPWU010000000
VERSION     DAOPWU010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15277_length_5376_cov_3.541064"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..561)
                     /locus_tag="PKM23_11170"
     CDS             complement(4..561)
                     /locus_tag="PKM23_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92073.1"
                     /translation="MTRSIIHIDVGDFPIAVERVLEPRLRQRPVVVAVQTATRSLVAA
                     LSREAAGQGVQRGMPLPQARKYCRDLTVLPPNEELYTRATTALLGLLGRFSPLIEPLR
                     FGHAYLDMTGSTRLFGGAVDAAARAQREIRDRLRLDAVAGVASNKLVSKVASDLLCRH
                     LPAAPLYDVHHGEEERFLSPPWCTS"
     gene            complement(575..1183)
                     /gene="lexA"
                     /locus_tag="PKM23_11175"
     CDS             complement(575..1183)
                     /gene="lexA"
                     /locus_tag="PKM23_11175"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNY92074.1"
                     /translation="MEKLSRKQEKALELIIKRAREQGYPPTLAELTEELGASSRNTAV
                     KYLRILARKGYIVWDRNKARGIQVAAEPGGEESGVPLIGSVAAGTPMLAEQNIERYVP
                     VPRFLLRAPGPHFLLRVSGESMINAGILNGDLVLVHARPQAEVGEVVVALIDSEATVK
                     RLAQREGRRFLHAENPAFADIHPAGEWSIQGKVVALLREEVL"
     gene            complement(1272..1517)
                     /locus_tag="PKM23_11180"
     CDS             complement(1272..1517)
                     /locus_tag="PKM23_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92075.1"
                     /translation="MVYDEILLEICADLAENIDSELCARLREHLQNCPNCARQFSTMR
                     TTIHLYHCLGEQEVPPAIHERLARLLNLPEGAAVHKR"
     gene            complement(1586..2539)
                     /locus_tag="PKM23_11185"
     CDS             complement(1586..2539)
                     /locus_tag="PKM23_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNY92076.1"
                     /translation="MAKDHWKQGGEPAMLRCYKIDKEKIQSCAREEAQILIYISPDDS
                     EKRYLTNEMKLDEHTLNSTLDPDELSRLEFEPEHFAMIYKRPRNYIVEDRFLFRVTSM
                     GIFFFSDRIILVASEDLPMFDDKKFSRVTSLRDVMLKMLYRSIYHFLEHLKIIDMITN
                     ELEEKINLSMQNKYLISLFSLEKSLVYYLNAITSNSILLQRLKNNSGKIAFQQEEEEL
                     LDDIMIENSQCYRQAEIFSNILSSMADARASIVSNNLNVLMKTLNIITIGIMVPTFVV
                     SAFSMNVSIPGQKLPYAFWLVLGIAFVSVMAFYFFWRYKKW"
     gene            2802..>5376
                     /locus_tag="PKM23_11190"
     CDS             2802..>5376
                     /locus_tag="PKM23_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNY92077.1"
                     /translation="MSKSNRKTAITLSLWGIFMLHAELRASQETGYRLPPREMIDLVD
                     APTTPSVSLSPDRQWLLVLDNPALPTIAELSQPELGLAGLRINPLSSGPSRISYHTGM
                     QLVSLRDNSTRPVLGLPPGARIRHAAWSPDSRRVAFTLTGESAITLWLLEIGSGEARE
                     LTGQPLSAAFYDDPFQWMPDSKRLLCRLIPMDRPEAPAAPAVPDGPVIQECGGESAPA
                     PTWQDLLRNPHDELLFDHYFSCLLALVDEEGNITRIGGPAIYRSAEPSPDGLAFLVEI
                     IHRPYSYLVPAMRFPHRVQIWDQHGVLIREMADLPLAEQVPIAFDAVPEGPRYFSWRA
                     DQPQTLCWCEAQDGGDPKRPAEIRDKVWTLAAPYGRDAELLANLSLRFRGVYWGNKHL
                     ALIQEWIWKERRVRIWAVDPAGRDRKGRLFADYSMEDRYKDPGTPLMTSTEWGTRVLR
                     LTRDDKAVYMRGEGASPEGSFPFLDERTIENGQSRRLWRCTAPYYEYVIDLASAGAES
                     FLTRREGIDEPPNFHLRRFGQESSVPVTSFPHPSPQLRGIRKELIRYERGDKVQLTAT
                     LYTPPGWERSQGPLPVLMWAYPQEYKSAAAASQVSDSPYRFIRISPLSPLFWVMRGYA
                     VVDDPAMPIVGEGDREPNDTYIEQLVASAEAIVAELKDRGVADSTRLAIGGHSYGAFM
                     TANLLSHCRLFKAGIARSGAFNRTLTPFGFQSEERTLWEAPETYLKMSPFMAAGTMDT
                     PILLIHGEADNNSGTFPMQSERYYAALKGLGKVARLVILPKESHGYRARESVLHMLWR
                     WTSGSNSISVRNEHPRSGGKGWDPFRRLRPSLLIEAPLPPLLDPSPRHPLALQPLLSC
                     KN"
BASE COUNT         1049 a         1608 c         1625 g         1094 t
ORIGIN      
        1 cctttacgat gtgcaccacg gcggggagag gaagcgttcc tcctcgccgt ggtgcacatc
       61 gtaaaggggg gcggcgggaa ggtgacggca gaggagatcg gaggcgacct tgctgaccag
      121 tttgttgctg gccaccccgg ccacggcatc gaggcgcagg cggtcgcgga tttcgcgctg
      181 ggcgcgggcg gcggcatcga ccgcgccgcc gaagaggcgg gtcgagccgg tcatgtcgag
      241 ataggcgtgg ccgaagcgca ggggttcgat gaggggcgaa aagcggccaa gcaggccgag
      301 cagggcggta gtggcgcggg tgtagagctc ctcgttgggc ggcagcacgg tcaggtcgcg
      361 gcagtatttg cgcgcctggg gcaggggcat gccgcgctgc acgccctgac cggccgcttc
      421 gcgggagagg gcggcgacca gtgagcgggt ggcggtctgg acggccacga ccaccggacg
      481 ctggcgcagg cggggttcca ggacccgttc cacggcgatg gggaaatccc cgacatcgat
      541 atggatgatg gaacgcgtca cggcatactc ctggctagag cacctcttcg cgcaacaggg
      601 cgacgacctt gccctggatg ctccactccc ccgccggatg gatatcggcg aaggcgggat
      661 tctcggcatg gaggaagcgt ctgccctcgc gctgggccag gcgtttgacg gtggcctcgc
      721 tgtcgatcag ggccaccacc acctcgccga cctcggcctg gggccgggcg tgcaccagca
      781 ccagatcgcc gttcagaatg ccggcgttga tcatgctctc gccgctcacg cgcaggagga
      841 aatgggggcc cggggcgcgc aaaagaaagc ggggcacggg cacgtagcgt tcgatgtttt
      901 gttcagccag catcggggtt ccggcggcca cggaaccgat caggggcacg ccgctctcct
      961 cccccccggg ttcggcggcg acctggatgc ctcgggcctt gttgcggtcc cagacgatat
     1021 agcccttgcg agcgaggatg cgcagatact tgacggcggt gttccgggaa gaggcgccca
     1081 actcctcggt caattccgcc agggtcgggg gatagccctg ttcccgggcg cgtttgatga
     1141 tgagttcgag cgctttttcc tgtttgcgcg acagcttttc catacccctc caaataaaaa
     1201 agggtgaaat taatttcacc cttagtatac actttttttt gcttgcagtc aagcaaaaag
     1261 tgtccatatg gtcacctttt gtgcaccgct gcgccctcag ggaggttgag cagcctggcc
     1321 agccgttcat ggatggccgg tgggacctct tgctctccga ggcagtggta gagatggatg
     1381 gtggtcctca ttgtcgagaa ctgccgggcg cagttcgggc agttctgcag gtgttcgcgc
     1441 agtcgggcac agagctcgga atcgatgttt tcggcaagat cggcgcagat ctccagcagt
     1501 atctcatcgt aaaccaaaaa aacctctaac gcatgaccag atctttttat ctattggatg
     1561 cagcagatcc taaaggatac aaaatctacc attttttgta gcgccagaag aaatagaatg
     1621 ccatcaccga gacgaaggcg atgccgagta cgagccagaa ggcgtagggc agtttttgtc
     1681 ccgggatgct gacattcatg gaaaaggcgc tcacaacgaa ggtcggcacc atgatgccaa
     1741 tggtgatgat gttgagggtc ttcatgagca cattgaggtt gttgctcacg atcgaggcgc
     1801 gcgcatcggc catgctggag aggatgttgg aaaagatctc ggcctggcgg tagcactgcg
     1861 agttttcgat cataatgtca tcgagcagct cctcctcttc ctgctggaag gcgatcttgc
     1921 ctgaattatt cttgaggcgc tggaggagga tggagttcga ggtgatggca ttgagatagt
     1981 agaccagcga cttttccagc gagaagaggc tgatcaggta cttgttctgc atggagaggt
     2041 tgatcttttc ctcgagttcg ttggtgatca tgtcgatgat cttgaggtgt tcgagaaagt
     2101 ggtagatgga acgatagagc atcttgagca tcacatcgcg caggctggtc acacgcgaga
     2161 actttttgtc gtcaaacatc ggcagatctt cgctggcaac caggatgatg cggtcgctga
     2221 aaaaaaagat gcccatcgag gtgacgcgaa agaggaagcg gtcctcgacg atgtaattgc
     2281 gcggccgctt gtaaatcatc gcaaaatgct cgggctcgaa ttcgaggcgc gagagttcat
     2341 ccggatcgag cgtggagttg agcgtgtgct catccagctt catctcgttg gtcagatatc
     2401 gcttttccga gtcgtccgga ctgatataga tgagaatctg cgcctcttca cgggcgcagc
     2461 tctggatttt ctctttatcg attttatagc agcgcagcat ggccggctct ccgccctgtt
     2521 tccagtgatc ttttgccaat gggtaagata cggatattat tttgatattg ccaacataat
     2581 attggccggt ccgatcaggg ccgaaggtaa taaaaagagt tgcaaatctg cctgtaattg
     2641 ctttatttaa attagattgc ttccattgcc ttttctttcc agctgcatca ttgctcaggc
     2701 agacccgccc ttgcaacttc ccttagctta cgtgccggcg acacaccgtc gcccagagcc
     2761 gggctcatcc cggttggatg gggcgaactg tggagggaac catgagcaaa tcaaacagaa
     2821 agacagcgat cactctctcc ttgtggggga ttttcatgct gcatgcagaa ctacgtgcat
     2881 cacaggagac gggctatcgc ctgcctcccc gcgagatgat tgatctggtc gatgcaccga
     2941 caacgccgtc ggtcagcctc tcgcctgacc gccaatggct gctggtgctg gacaatcccg
     3001 ccctgcccac cattgcagag ctctcccagc ctgagcttgg tctggccggc ctccgcatca
     3061 atccgctcag cagcggaccg agccgaatca gctatcacac cggcatgcag cttgtgtcgc
     3121 tgcgcgacaa ttcaacccga ccggtgttgg ggctcccccc cggtgcccgc atccgccatg
     3181 ccgcctggtc tcccgacagc cgcagggtgg ccttcaccct caccggcgag agcgcgatca
     3241 ccctctggct gctcgagatc ggcagcggag aggcgcgtga attgaccggc cagcccctca
     3301 gtgccgcatt ttatgacgat cccttccagt ggatgcccga cagcaaacga ctgctctgcc
     3361 ggctgatccc gatggatcga cctgaagcgc cggccgctcc ggctgtaccg gatggaccgg
     3421 taatccagga atgcggaggt gaaagcgcac cagcgccgac ctggcaggat ctgctccgga
     3481 atccgcacga cgagctgctt ttcgaccact atttcagctg ccttctggcg cttgtcgacg
     3541 aagaggggaa tatcacccgg attggcgggc cggccatcta tcgctcagcg gagccctccc
     3601 ctgacggact cgcctttttg gtcgagatca tccatcgtcc ctactcctat ctggtgccgg
     3661 cgatgcgctt cccccaccgg gtgcagatat gggatcagca tggagttctc atcagggaaa
     3721 tggccgacct tccgctcgcc gaacaggtgc cgatcgcctt cgatgccgta ccggaggggc
     3781 cgcgctattt cagctggcgc gccgatcagc cgcagacgct ctgctggtgc gaagcccagg
     3841 atggcgggga tcccaaacgt cccgcagaaa ttcgcgacaa ggtctggacc ctcgcggctc
     3901 cctacggccg cgacgccgag ctgctcgcca atctctccct gcgtttccgc ggggtctact
     3961 gggggaacaa gcacctcgcc ctgattcagg agtggatctg gaaggagcgg cgagtgcgga
     4021 tctgggctgt cgacccggct ggccgagacc gaaagggccg cctcttcgca gactattcca
     4081 tggaggaccg ctacaaagat cccggcacgc ccctcatgac ctccacggag tggggcacgc
     4141 gtgttcttcg cctcacccgc gatgataaag ccgtctacat gcgcggcgaa ggcgcctccc
     4201 ccgaagggag cttccccttc ctggatgagc gcaccatcga gaacggccag agccgtcgcc
     4261 tgtggcgctg cacagcgccc tattatgaat atgtcatcga cctggcaagc gcaggagcgg
     4321 agagcttttt aacccggcgg gaagggatcg atgaaccccc caattttcac ctgcgccgct
     4381 ttggacaaga gtcctcggtg ccagtgacct cttttcctca tccctctccc cagcttcgtg
     4441 gcatccgcaa ggagctgatc cgctacgaac ggggtgacaa ggtccaactt acagccacgc
     4501 tctacacccc acccggatgg gaacgcagcc aggggcccct gcctgtgctg atgtgggcct
     4561 atccccagga gtacaagagc gcggccgcag ccagccaggt ttccgattca ccgtaccgtt
     4621 ttatccgcat ctcccccctc agccctctgt tctgggtgat gcgcggatac gcggtggtcg
     4681 atgatccagc catgccgatt gtcggtgagg gcgaccggga gcccaacgac acctacatcg
     4741 agcagctggt tgccagtgct gaggccatcg tcgcggaact gaaggaccgc ggtgtggctg
     4801 attcgacccg tctggcgatt ggagggcaca gttatggcgc tttcatgacc gccaatctgc
     4861 tcagccactg ccgtctcttc aaggccggca tcgcccgcag cggggctttc aaccgcaccc
     4921 tcaccccctt cggctttcag agcgaggagc gcactctttg ggaggccccg gagacttatt
     4981 taaagatgtc acctttcatg gcggccggga cgatggatac ccccatcctg ctcatccacg
     5041 gcgaggcgga caacaacagc ggcaccttcc cgatgcagag cgaacgctat tatgccgcgc
     5101 tcaagggact cggcaaggtc gcccgactgg tcattctgcc aaaggagagc cacggctacc
     5161 gggcgcggga atcagtcctg cacatgctct ggagatggac cagtggctca aattccatct
     5221 cggtgagaaa tgagcatcct cgatcaggtg gcaagggttg ggatcctttc cgccgtctcc
     5281 gcccatccct cctgatcgag gcccctctgc cgccgctgct tgacccttct ccccgacacc
     5341 cccttgccct gcaacccctg ctatcatgta aaaaca
//
LOCUS       DAOPWU010000238         5354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15351_length_5354_cov_4.721079, whole genome shotgun sequence.
ACCESSION   DAOPWU010000238 DAOPWU010000000
VERSION     DAOPWU010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15351_length_5354_cov_4.721079"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PKM23_11195"
     CDS             <1..908
                     /locus_tag="PKM23_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNY92078.1"
                     /translation="VKVVWSREDDMRHGFFRPASFHRVTGLLDAGKKPVGWHHHIVAP
                     SISVQLFGEGDGRGNPDAVDGAAQLPYRMGNIHIDYVMANTAVPVTWWRSVYNSQNAF
                     VNECFMDEMAHAAGADPVQFRLDLLPADSRLRAVLQLAADKSEWGKSLPPGRGRGIAC
                     HACFGSFAAHCAEVEVDGSGRLRVTRYVCAIDCGQAVNPDGVKAQMEGGVIFALSAAL
                     RGQITIAKGGVVQGNYDTYEPLRINESPEILVHLVPSHLPPGGVGEPGVPPVAPALCN
                     AIFAATGVRVRQLPVSSTSLMRSGE"
     gene            1004..2074
                     /locus_tag="PKM23_11200"
     CDS             1004..2074
                     /locus_tag="PKM23_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNY92079.1"
                     /translation="MIWLWIGFIGFVLAMLALDLGVFHRKAHVVQMKEALGWSGVWIA
                     LGLAFSFFIYHAYNAHWFGLGTAVDAVDGLVNSGRTAAIKYLTGYVVEKSLSVDNIFV
                     IAMIFGFFAVPAIYQHRVLFWGILGALVMRGAMIGVGAALIANFHWNLYIFGGFLILT
                     GAKMLFMHGGAEDPNRNVMVRLVRRFFPVTERFHGQHFIVRAGSAASRKGVLPGDDEA
                     DDTVVGSVKKGAVLLTPLALALIVVEATDLIFAVDSIPAIFAITADPFLVFTSNVFAI
                     LGLRSLYFALAGMLDKFRYLKVALAVVLMVVGAKMLTATWLKSVLGDHFNFILLGVIL
                     SILAAGILLSLLANRRDARRAG"
     gene            complement(2081..>5354)
                     /locus_tag="PKM23_11205"
     CDS             complement(2081..>5354)
                     /locus_tag="PKM23_11205"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNY92080.1"
                     /translation="FALCAASCLFTLSTGAAAAPAAPQLLFEQINVEQGLSQSIVTNI
                     MQDRRGYLWFGTEDGLNLYDGYGFSVLRSDPDDPNSLSYNQITALYEDRTGSIWAGTF
                     NGGLNRYQLEKNRFIRFRNSLADNSSLSNDLVYAIVQDRAGQLWVGCDHGLNRMKNPH
                     AAEGSGRFDRFFHSPGDKASLRDDHVRALAVDSIGTLWAGTDQGLAMLPAAELLHDKP
                     RFTVPEMQEKEWNALAQDTIRTLMVDRSGALWIGAVHGLHCLRREPGRPPRLQSFFHT
                     PGNPRTLSNNHVYALLEDAEGNIWVGTEGGLNRFDPRSGEFAAWLNDPRNPHSLSHNE
                     IRSLFQDRSGLLWVGTYGGGVSKADARPKPFVHYRYLPEVPEGLSQPIIWSIYEDANG
                     ILWIGTHGGGLDRLERRTGRYTHYRANPADPHSLSHNIVRLVIPGRDGELWIGTHGGG
                     ICRFNPATGRFVRYLHDPQNPYSLCHNEIRALFLDSDSTLWIGTQGGGLDRLDTRKLN
                     TASPRFRHLRSDPGDSNSIASDYIRFIHKDREGCYWIGTQGGGLDRYDAAAGRFSHYR
                     STPGAQGGINSNYVFCLHEDRQGTFWIGTWGAGLIRFDRKTGIFTAYRTRDGLPSDAI
                     YGILEDGRGRLWFSTNNGIGRFDPETAECKNYSIRDGLQSNEFNGGSFFLSPSGEMFF
                     GGINGFNSFFPEQIRDNPHIPPVVITGFKKMNRQVRLDLPLSEIRHLKLSHRDYFFSF
                     EFAALDYTAPEKNRYAYKMEGLDQEWITTSADQRVAQYTTLPAGSYLFRVKGSNNDGV
                     WNEEGCSIAISIAPPFWRTWWFRLLGLLILCTIALFYFRRRLGIIRMAAELSAAHEMQ
                     MAIMPHTGPQVPGFDISGLCIPANQVGGDFFDYAWVGSDRKRFGIMIGDVSGKAMRAA
                     MTAVMANGIVHAEAAEGKSPAEALTRSNEILYPKLERHTFIALCLVALEPENRAMCFT
                     NAGLSEPLLYSDGAWRILRGSGPPYPIGVRQGHLYQDKRVRLLPGEIIILQTDGIQDA
                     MNRSKAFYGEERLLTFLRGLDCAALNAQQIRDSIIADVQAFTNGVHPFDDMTVIVIRV
                     LE"
BASE COUNT          915 a         1577 c         1813 g         1049 t
ORIGIN      
        1 ccgtcaaggt ggtctggagc cgtgaggacg acatgcgcca cggctttttc cgccctgcca
       61 gttttcacag ggtgacaggg ctgctcgacg ccgggaagaa accggtcggc tggcaccacc
      121 atatcgtcgc cccctcgatc tcggtccagc tcttcggaga gggagacggc cgcggcaatc
      181 ccgacgccgt ggacggggcg gcccagctcc cttaccggat gggaaatatt catatcgatt
      241 acgtcatggc caacaccgcg gtgccggtga cctggtggcg ttcggtctac aattcccaga
      301 atgcctttgt caatgaatgc ttcatggatg agatggccca cgccgccggc gccgacccgg
      361 tgcagttccg tctggatctg ctgccggcgg actcgcgcct gcgcgcggtg ctccagctcg
      421 ccgctgacaa gtcggagtgg ggcaaatccc tgcccccggg ccggggccgc ggtatcgcct
      481 gtcacgcctg ctttggcagc tttgccgccc actgcgccga ggtggaggtg gatggcagcg
      541 gccgcctgcg ggtgacgcgc tacgtctgcg ccatcgactg cgggcaggcg gtgaatccgg
      601 acggggtcaa ggcccagatg gagggagggg tgatctttgc cctctcggcc gcgttgcgcg
      661 gccagatcac catcgcgaag ggcggggtgg tgcagggcaa ctatgacact tatgaacccc
      721 tgcgcatcaa cgagagcccg gagattttgg tgcatctcgt ccccagccat ctgccccccg
      781 gcggagtggg ggaaccgggg gtgccgccgg tggcgccggc gctgtgcaac gccatctttg
      841 ccgcgaccgg cgtccgggtc cgtcagctcc cggtctcctc gacctcattg atgcgcagcg
      901 gggaatagaa gcaccccggc tgctgttaaa ttgaccaaaa tcggaagcaa gcgcgtggga
      961 cggagacgcc cgcgcgcttt tctataaagt ggaaggataa caaatgatct ggctctggat
     1021 tggattcatc ggcttcgttc tggccatgct cgccctcgat ctcggggttt ttcaccgcaa
     1081 ggcccatgtc gtgcagatga aggaggctct gggctggtct ggggtctgga tcgccctcgg
     1141 cctggctttc tcgttcttca tctaccatgc ctataacgcc cactggttcg gccttggcac
     1201 ggccgtggat gccgtcgacg gcctggtcaa ttccggacgc accgccgcta tcaagtatct
     1261 gaccggctat gtcgtcgaaa aatccctgag cgtcgacaac atcttcgtca tcgccatgat
     1321 tttcggtttc ttcgctgtgc cggccatcta tcagcaccgg gtgctcttct ggggcattct
     1381 cggcgccctg gtgatgcgcg gggcgatgat cggcgtcggt gcggccctga tcgccaactt
     1441 ccactggaat ctctatatct tcggagggtt tctcatcctc accggggcca agatgctttt
     1501 catgcatggc ggcgccgagg atccgaaccg caacgtcatg gtgcggctgg tgcgccgctt
     1561 tttccccgtc actgaacgtt ttcacggcca gcatttcatc gtgcgcgccg gctcggccgc
     1621 ctcgcgcaag ggggtgcttc ccggcgatga cgaggccgac gacaccgtgg tcggcagcgt
     1681 caaaaagggg gcggtgctgc tcacccccct cgccctggcc ctgatcgtgg tcgaggcgac
     1741 cgacctgatc tttgcggtgg actcgatccc cgcgattttt gccatcaccg ccgatccctt
     1801 tctggtcttc accagcaatg tctttgccat tctcggcctg cgttcgctct atttcgccct
     1861 ggccggcatg ctggacaaat tccgttatct caaggtcgct ctggccgtgg tgctgatggt
     1921 ggtgggcgcc aagatgctga ccgcgacatg gctcaagagt gtgctcggcg accatttcaa
     1981 tttcatcctg ctcggcgtga tcctctccat cctcgccgcc gggatcctgc tctccctgct
     2041 cgccaaccgc cgcgatgcgc ggcgggcggg ctgaaaggct tcactccagc accctgatca
     2101 cgatcaccgt catgtcgtcg aaagggtgaa ccccgttggt gaaggcctgg acatcggcga
     2161 taatgctgtc gcggatctgc tgcgcgttca gggcggcaca gtcgagccct ctgagaaaag
     2221 tgagcaggcg ctcctcgcca tagaaagcct tgctccggtt catcgcatcc tgaatgccgt
     2281 cggtttgcag gataatgatt tccccgggca ggagccgcac ccgcttatcc tgatagagat
     2341 ggccctggcg cacaccgatg gggtagggcg ggccgctgcc gcgcaggatc cgccatgccc
     2401 catctgagta gaggaggggt tcactcagtc ccgcattggt gaagcacatg gcccggttct
     2461 ccggctcaag ggcgaccagg cagagggcga tgaaggtgtg gcgttcgagc ttggggtaga
     2521 ggatctcgtt gctgcgcgtg agcgcctccg ccgggctctt gccctccgcc gcttcggcat
     2581 ggacgatgcc gttggccatg acagcggtca tcgccgcccg catcgctttt ccagagacat
     2641 ccccgatcat gataccgaac cgtttccggt cacttcccac ccaggcataa tcgaagaaat
     2701 ccccgccgac ctgattggcc ggaatgcaga gccccgaaat gtcgaatccg ggcacctgcg
     2761 gcccggtgtg cggcatgatc gccatctgca tttcgtgggc cgcggagagt tcggccgcca
     2821 tgcggataat gcccaggcgc cggcggaaat aaaagagggc gatggtgcag aggatgagga
     2881 ggcccaggag gcggaaccac caggtccgcc agaagggcgg agcgatggag atggcgatcg
     2941 agcacccctc ctcgttccag accccgtcgt tgttggagcc cttgacgcgg aagagatagc
     3001 tgcccgccgg cagggtggtg tactgcgcca cgcgctggtc cgccgaggtg gtgatccact
     3061 cctggtccag cccctccatt ttgtaggcat agcggttctt ctcgggcgcg gtataatcga
     3121 gggcggcgaa ctcgaaggag aaaaagtagt cccggtggga gagcttgagg tgcctgatct
     3181 cggagagggg gaggtcgagg cgcacctgcc ggttcatctt tttaaagccg gtgatgacca
     3241 ccggcgggat atggggattg tcgcggatct gctcggggaa aaagctgtta aagccgttga
     3301 tgcctccgaa gaacatttca ccactcgggc tgagaaagaa ggagccgccg ttgaattcgt
     3361 tgctctgcag gccgtcgcgg atgctgtaat ttttacactc tgcagtttcg ggatcgaagc
     3421 gcccgatgcc gttattggtg ctgaaccaga gccgtccccg gccgtcctcg aggattccgt
     3481 atatggcatc ggagggcagg ccgtccctgg tgcgataagc ggtgaagatg ccggttttac
     3541 ggtcgaatct gatcaggccg gcgccccagg tgccgatcca gaacgttccc tgccggtctt
     3601 catgcaggca gaagacatag ttgctgttga tgcctccctg agcacccggg gtgctgcgat
     3661 aatgggaaaa gcggccggcc gcggcgtcgt agcggtcgag gccgcccccc tgggtgccga
     3721 tccagtagca accctcgcgg tccttgtgga tgaagcggat gtagtcgctg gcgatggagt
     3781 tgctgtcccc gggatcggag cggaggtggc ggaagcgggg ggaggcggtg ttgagcttgc
     3841 gagtatcgag ccggtcgagt ccgccgccct gggtgccgat ccagagggtg ctgtcgctgt
     3901 cgagaaagag ggcgcggatc tcattgtgac acagggagta gggattttgg gggtcgtgca
     3961 ggtagcggac aaaacggccg gtggcaggat tgaagcggca gatgccgccg ccgtgagttc
     4021 cgatccagag ttcgccatcg cggccgggta tgaccaggcg gacgatattg tggctgaggg
     4081 aatgggggtc ggccgggttg gcgcggtaat gggtgtagcg gcccgtgcgg cgctcgagcc
     4141 ggtcgagacc gccgccatgg gtgccgatcc agaggatgcc gttggcatct tcatagatcg
     4201 accagatgat gggctggctg agcccctccg gaacctccgg cagatatcgg taatgaacga
     4261 agggcttcgg ccgggcgtcg gccttgctga ccccgccgcc gtaggtgccc acccagagca
     4321 ggccggagcg gtcctggaag agggagcgga tctcgttatg gctcagggaa tggggattgc
     4381 ggggatcgtt gagccaggcg gcgaactcgc cgctgcgcgg gtcgaagcgg ttgaggcccc
     4441 cttcagtgcc aacccagata ttaccctcgg catcctcgag cagggcgtag acgtggttgt
     4501 tgctgagggt gcggggattg ccaggtgtat ggaaaaagct ctgcaggcga ggagggcggc
     4561 ccggctcccg gcgcaggcag tgcagcccat gcactgcgcc gatccagagg gcgcccgagc
     4621 ggtcgaccat gagggtgcgg atggtgtcct gagccagggc attccactcc ttctcctgca
     4681 tctccggcac ggtaaatcgg ggtttgtcgt ggaggagctc cgcggccggg agcatggcca
     4741 gcccctggtc ggttccggcc cagagggtgc cgatgctgtc gacggcgagg gcgcgcacat
     4801 ggtcatcccg gagcgaggct ttgtcgcccg gagaatgaaa aaagcggtca aaacggcccg
     4861 atccctctgc agcgtgggga tttttcatcc ggttgaggcc gtgatcgcac cccacccata
     4921 gctggccggc gcggtcctgg acgatggcgt agacgaggtc gttgctgagc gaactgttat
     4981 cggcgaggga gttgcggaag cggatgaaac ggtttttctc cagctgatag cggttgagcc
     5041 cgccgttgaa ggtgcccgcc cagatcgagc cggtgcggtc ttcatagagg gcggtgatct
     5101 ggttgtagct gaggctgttg ggatcgtccg ggtcggagcg cagaaccgaa aaaccgtagc
     5161 cgtcatagag attgaggccg tcctcggtgc cgaaccagag ataaccccgc cggtcctgca
     5221 tgatgttggt gacgatgctt tgggagaggc cctgctcgac gttgatctgc tcaaagagca
     5281 gctgcggcgc ggcgggtgcg gcggcggccc cggtcgagag ggtgaaaagg caggatgcgg
     5341 cgcagagcgc gaaa
//
LOCUS       DAOPWU010000239         5326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15444_length_5326_cov_4.213432, whole genome shotgun sequence.
ACCESSION   DAOPWU010000239 DAOPWU010000000
VERSION     DAOPWU010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15444_length_5326_cov_4.213432"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKM23_11210"
     CDS             complement(<1..199)
                     /locus_tag="PKM23_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92081.1"
                     /translation="MNKSPLLLLLLLTSGCGIYSVKGSMAPHLKTVAIPLFDNRTAEF
                     RVAEDLTDAVINSFTRDNSLKI"
     gene            complement(192..1370)
                     /locus_tag="PKM23_11215"
     CDS             complement(192..1370)
                     /locus_tag="PKM23_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY92082.1"
                     /translation="MSTDLAPNSRWQEVQQQVGILGRSEAIRRLLETIEQVAQTDISV
                     LITGESGTGKELVARAIHARSRRRLNPLITVNCGAIPEGIIESELFGHEKGSFTGAIG
                     PRKGYFELADKGSIFLDEIGELPLTTQVKLLRVLESREFMRVGGVTSVQTDVRFIAAT
                     NKQLEEEVRRGNFREDLFYRLNAVHIRVPALRERSEDIPILVRKFAQEFVRENHIDFG
                     GFSESALAAMAHARWPGNIRELRNVVEKIIVLERGAYVEEATIRRYLNISHPMDPALP
                     VPLKRGREESERDLLLRVLLEIKSEIAQLRELLLRRQAAPYPLAPWREDLAEEYLEPA
                     QPPADGEHSSVAEMERELIQNALLKFGGSKRRAARALGLSERTLYRKIEKYGLKGTHE
                     "
     gene            complement(1378..2301)
                     /locus_tag="PKM23_11220"
     CDS             complement(1378..2301)
                     /locus_tag="PKM23_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNY92083.1"
                     /translation="MKILVTGGAGFIASQITDAYIAAGHQVVVVDNLSTGRRENLNPD
                     AVFYEMDIQDPRLIEVFQRHEIEIVNHHAAQMDVRRSVEDPIYDARNNVLGFLNILQA
                     CARTGVKRVIFSSSGGAIYGEQDYFPADEGHKTQPCSPYGITKLVGEKYLFFYSQNYG
                     IEHTILRYANVYGPRQNPHGEAGVVAIFCSRLLAGEEPVINGTGEQTRDFVFVGDVVA
                     ANLLALGLKGNDTFNIGTARESTINEVYRILNQLIGSNKPEKHGPGKEGEQFRSVIDP
                     SHALKVMGWKPRFSLDTGLRETVDFFRKAKA"
     gene            complement(2446..3717)
                     /gene="purD"
                     /locus_tag="PKM23_11225"
     CDS             complement(2446..3717)
                     /gene="purD"
                     /locus_tag="PKM23_11225"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNY92084.1"
                     /translation="MRVLIIGSGGREHALVWKVKRSALVKKVYCAPGNYGISCDALCI
                     DIPAHDIKRLLAFAVKEKIDLTIVGPEQPLVAGLVDEFEANGLAIFGPNQRAAELEGS
                     KAFAKELMARHKIPSAEFRIFTALEEAQNYVNSARMPLVIKADGLAAGKGVYVCTEKV
                     QALIALHQIMESRVFGQAGSRVVIEEFLTGEELSVLALSDGENLVYLAPAQDHKRIFD
                     GDLGANTGGMGAYAPTPFLDEETFRRIRTEIMEPTIRAMAREDRPYRGVLYAGLILTS
                     EGPRVLEYNCRFGDPETQVILPLAASDLVEAIMACRAGGLANFQWRNHAGAAAGVVIA
                     SGGYPERYTTGHPIVGLEGPLEKDVVIFHSGTKAAEGHPVTAGGRVLTVTAWAPDLEA
                     AIARAYRTVGKLTFDGAYYRRDIGARGLRKR"
     gene            complement(3726..>5326)
                     /gene="uvrB"
                     /locus_tag="PKM23_11230"
     CDS             complement(3726..>5326)
                     /gene="uvrB"
                     /locus_tag="PKM23_11230"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNY92085.1"
                     /translation="PEDWLEMLIILKTGQKIERNTILQKLVEVHYARNDTDFDRGTFR
                     VRGDVVEVIPAYEREAIRIEMFGDEIERISMVDPLTGEIRSETDTVAIYPAKHFVTSP
                     ERLKKAMKGIEDELQARLTDFRSRNKLLEAQRLEQRTRFDLEMMQEIGYCSGIENYSR
                     HLGGRNPGESPSTLIDYLPEDYLLIIDESHVTVPQIRGMFHGDRSRKETLVEFGFRLP
                     SALDNRPLNFDEFTAAINQCIFVSATPADYELEMCQGIVVEQVIRPTGLMDPEIEVRP
                     IKGQIDDLIGEINERAGRGERILVTTLTKRMAEDLTEYLAGMGIRVRYMHSEIEALER
                     VAILRDLRLAEFDVLVGINLLREGLDLPEVSLVAVLDADKEGFLRSERSLIQTAGRAA
                     RNVNGKVLFYADKITDSMRMAMEETSRRRTIQAEYNQIHNITPTSIVKSVDDVMGATR
                     VADARSEPYGKKEANVDDKEFRQAWARMSAKEKLELMSEMEKKMFAAAAALQFEEAAE
                     LRDRIDLLRLSYEEKHKIEQILAK"
BASE COUNT          908 a         1580 c         1755 g         1083 t
ORIGIN      
        1 cgatcttgag cgaattgtcc cgggtgaagg aattgatcac cgcgtcggtc aaatcctcgg
       61 cgacgcggaa ttcggcggtg cggttatcga aaagagggat ggccaccgtc ttgaggtgcg
      121 gtgccatcga gcccttgacg gagtagatgc cgcagcctga ggtgaggagc aggagaagca
      181 gaagaggact tttattcatg ggtgcccttc agaccatatt tttcgatttt gcgatagagg
      241 gtgcgctcgc tcaggcccag ggcgcgggcg gccctgcgct tgctgccgcc gaatttcagc
      301 agggcgttct ggatcagctc cctctccatt tcggccaccg aactgtgctc cccgtcggca
      361 ggaggctggg cgggctcgag atactcctcg gcgagatctt cccgccacgg ggcgagggga
      421 taaggagcgg cctggcgccg cagcagcagt tcgcggagct gggcgatctc gctcttgatc
      481 tcgagcagta cgcgcagcag gaggtcgcgc tcgctctcct cacgcccgcg cttgagggga
      541 accggcaggg ccgggtccat gggatggctg atgttcagat agcgccggat ggttgcctct
      601 tcgacgtagg ccccgcgttc caggacgatg atcttttcga ccacattgcg cagttcgcga
      661 atgttgcccg gccagcgggc gtgggccatg gcggccaggg ccgactcgct aaagccgccg
      721 aaatcgatgt ggttttcgcg gacgaattcc tgggcaaatt tccggaccag gatcgggatg
      781 tcctcgctgc gctcgcgcag cgccggcacc cggatatgga cggcattgag gcggtaaaag
      841 aggtcctcgc ggaagttgcc gcggcgcacc tcctcctcga gctgcttgtt ggtggcggcg
      901 atgaaacgca cgtcggtctg gacggaggtc actccgccga cgcgcatgaa ttcgcgcgac
      961 tcgagcacgc gcagcagctt gacctgggtg gtaaggggaa gttcaccgat ctcgtcgaga
     1021 aagatggagc ccttatcggc gagctcgaag tagcccttgc gcggcccgat cgccccggtg
     1081 aaggagccct tttcgtgtcc gaagagttcg gattcaatga tcccttcggg gatggccccg
     1141 cagttgaccg taatgagggg attcagccgc ctccggctgc gggcgtggat cgccctggct
     1201 accagttcct tgccggtgcc gctctcgccg gtgatgagca ccgagatatc ggtctgggcc
     1261 acctgctcga tggtctccag caggcggcgg atggcctcgg agcggccgag gatgccgacc
     1321 tgctgctgaa cctcctgcca gcggctgttg ggagccagat ccgtgctcat gcccgcctca
     1381 ggcctttgct ttcctgaaaa aatcgacggt ctcgcgcagt ccggtgtcga ggctgaagcg
     1441 gggcttccac cccatcacct tgagcgcgtg ggagggatcg atgacgctgc ggaactgctc
     1501 gccctccttg ccggggccgt gtttttcggg tttgttgctg ccgataagct ggttgaggat
     1561 ccggtagacc tcgttgatcg tgctctccct ggcggtgccg atgttgaagg tatcgttgcc
     1621 cttgaggcca agggcgagga gattggcggc cacgacgtcg ccgacaaaga cgaaatcccg
     1681 cgtctgctcc ccggtgccgt tgatgaccgg ctcctcgcct gccagcagcc ggctgcaaaa
     1741 aatcgccacc acgcccgcct ctccgtgcgg attctggcgc ggcccgtaaa cattggcgta
     1801 gcggaggatg gtgtgctcga tgccatagtt ctggctgtag aaaaagaggt acttctcgcc
     1861 gaccagcttg gtgatgccat aggggctgca gggctgggtc ttgtgccctt catcggccgg
     1921 aaaatagtcc tgttcgccat agatcgcccc gccggaggag gaaaagatga cccgtttgac
     1981 cccggtcctg gcgcaggcct gcaggatgtt gagaaagccg agcacattgt tgcgggcgtc
     2041 gtagatgggg tcttcgacgg agcggcgcac atccatctgg gcggcgtggt gattcacgat
     2101 ctcgatctca tggcgctgaa agacctcaat gagccggggg tcctggatat ccatctcgta
     2161 aaagaccgca tcgggattga ggttttccct cctgcccgtg ctcaggttat ccaccactac
     2221 gacctgatgg ccggcggcga tataggcatc ggtgatctgc gaggcgataa aaccggcgcc
     2281 gccggtgacg aggattttca tgctgcgctc cttaataata gggcttctgg tttcgaagaa
     2341 ggggaagggc tgcggaagcc tgacctctgg tgcggtgcgg cccactctgc ttcaggtgat
     2401 ctgctcttta gactatactc caaaagtgcg ggaatgcaga ggggattatc gcttgcgcaa
     2461 tcccctggcc ccgatgtccc gccggtaata ggcgccgtcg aaagtcagct tgcccaccgt
     2521 ccggtaggcg cgggcgatgg ccgcctccag gtccggcgcc catgccgtga cggtgaggac
     2581 gcggccgccg gcggtaaccg gatggccctc cgccgctttt gtgccggaat ggaagatgac
     2641 gacatccttc tccagtggcc cctccagtcc gacgatggga tggccggtgg tataacgttc
     2701 cggatagccc cccgaggcga tgaccacgcc ggcggcggcg ccggcatgat tccgccattg
     2761 aaagttcgcc agtccccctg cgcggcaggc catgatcgcc tcgaccagat cgctggccgc
     2821 caggggcagg atcacctggg tctcgggatc gccgaagcgg cagttgtact ccagaaccct
     2881 gggcccctca ctggtgagga tgaggccggc gtagagcaca ccccggtagg ggcggtcctc
     2941 gcgcgccatc gcccggatgg tcggttccat gatctccgta cggatgcggc gaaaggtctc
     3001 ctcatcgagg aagggggtgg gggcataggc gcccatgccg ccggtattgg cgcccagatc
     3061 gccgtcaaag atccgtttgt ggtcctgcgc cggggccaga tagaccaggt tctctccgtc
     3121 gctgagagcg agcacggaga gttcctcgcc ggtgaggaac tcttcgatga cgacgcggct
     3181 gcccgcctgg ccgaagaccc ggctctccat gatctggtgg agggcgataa gggcctgcac
     3241 cttttccgtg cagacataga cccctttgcc cgcggccagt ccgtcggcct tgatcaccag
     3301 gggcatgcgg gcgctgttga cataattctg ggcctcctcg agtgcggtga agatgcggaa
     3361 ttcggcggag ggaattttgt gccgcgccat cagctccttg gcgaaggcct tgctgccctc
     3421 gagttcggcg gcgcgctggt tggggccgaa gatggccagg ccgttggctt cgaactcgtc
     3481 gaccagtccg gcgaccaggg gttgttcggg accgacgatg gtgagatcga tcttttcctt
     3541 gacggcgaag gcaagcaggc gcttgatatc atgcgccggg atgtcgatgc agagggcatc
     3601 acaggagatg ccgtaattgc cgggcgcaca gtagaccttt ttgaccagcg cactgcgctt
     3661 caccttccag accagtgcgt gttcgcgtcc tccgctgccg atgatcagta ctcgcataac
     3721 gctccctatt tggccagaat ctgctcaatc ttgtgttttt cttcgtagct gaggcggagg
     3781 aggtcgatcc ggtcgcgcag ctccgccgcc tcttcgaact gcagggccgc cgcggcggca
     3841 aacatctttt tctccatctc gctcatcagc tcgagctttt ccttggcgct catacgggcc
     3901 caggcctggc ggaattcctt gtcatcgaca ttcgcctcct tcttgccgta gggctcggaa
     3961 cgggcgtcgg ccacgcgcgt ggcacccatg acatcatcca ccgatttgac aatcgaggtg
     4021 ggggtgatat tgtggatctg gttgtattcc gcctggatcg tgcggcgccg actggtttcc
     4081 tccatcgcca tgcgcatcga atcggtgatc ttgtcggcgt aaaaaagcac cttgccattg
     4141 acgttgcgcg cggcgcggcc ggcggtctga atgagggaac gctcggagcg gaggaatccc
     4201 tccttgtcgg cgtcgaggac ggcgaccaga gagacctcgg gcaggtcgag cccctcgcgc
     4261 agcaggttga tgccgaccag aacatcgaat tcggcgaggc gcaggtcgcg gaggatggcg
     4321 acgcgctcga gggcctcgat ctcggagtgc atgtagcgga cgcggatgcc catgccggcg
     4381 agatactcgg tcaggtcctc ggccatacgc ttggtcaggg tggtgaccag gatgcgctcc
     4441 ccgcgcccgg cgcgctcgtt gatctccccg atcaggtcgt cgatctggcc cttgatcggc
     4501 cggacctcga tctcgggatc catcagtccg gtggggcgga tcacctgctc gacgacgatg
     4561 ccctggcaca tttccagttc ataatcggcc ggggtggcgg agacgaagat gcactggttg
     4621 atcgcggcag tgaattcgtc gaaattgagg ggtcggttgt cgagggccga ggggagccgg
     4681 aagccgaatt cgaccagggt ctccttgcgc gagcggtcgc cgtggaacat gccgcggatc
     4741 tgcggcaccg tgacatgcga ttcgtcgatg atgagcagat aatcctcagg caggtaatcg
     4801 atgagggtcg aggggctctc gcccggattg cggccgccga gatggcggga gtaattctcg
     4861 atgcccgagc agtagccgat ctcctgcatc atctccagat cgaagcgggt gcgctgctca
     4921 agccgctgag cctcgagcaa tttgttgcgg ctgcggaaat cggtgaggcg ggcctgcagc
     4981 tcatcctcga tccccttcat cgccttcttg agccgttccg gagaggtgac aaaatgtttg
     5041 gccggataga tcgccaccgt gtcggtttcg ctcctgatct cgccggtgag gggatcgacc
     5101 atcgatatgc gttcgatctc atccccgaac atctcgatgc ggatcgcttc gcgctcgtag
     5161 gcggggatca cctcgacgac atccccgcgc acccgaaagg tgccgcggtc gaaatcggtg
     5221 tcgttgcggg cgtaatggac ctccaccagc ttttgcagga tggtgttgcg ctcgatcttt
     5281 tggccggtct tgaggatgat cagcatctcc agccagtctt cgggcg
//
LOCUS       DAOPWU010000240         5284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15614_length_5284_cov_4.480589, whole genome shotgun sequence.
ACCESSION   DAOPWU010000240 DAOPWU010000000
VERSION     DAOPWU010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15614_length_5284_cov_4.480589"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /gene="ribA"
                     /locus_tag="PKM23_11235"
     CDS             <1..992
                     /gene="ribA"
                     /locus_tag="PKM23_11235"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNY92086.1"
                     /translation="AFTVSVDYRYGGVTTGISAADRAATILALTDPQVKPADFAKPGH
                     IFPIAAVTGGVLRRAGHTEAVVDLARVAGAYPAGVLCEIMDEDGSMARLPALFKFARQ
                     FDLRIITVRAMIEYRRRNEILINRVASTDFPTRFGHFALHVYESKVDDHHHLALVKGD
                     VAGEEPALVRVHSQCLTGDVFGSMRCDCGEQLEYALREIEKKGRGVLLYMRQEGRGIG
                     LAKKIMAYALQDQGKDTVEANEALGYKADLRDYGIGAQILWDLGVRKIALLTNNPKKI
                     IGLKGYGLEVVERVPIEVEPNSVNGFYLETKRDKLGHLIMMDEEGKQPDVQES"
     gene            1055..1525
                     /gene="ribE"
                     /locus_tag="PKM23_11240"
     CDS             1055..1525
                     /gene="ribE"
                     /locus_tag="PKM23_11240"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNY92087.1"
                     /translation="MPHVIEGQLSASGKKFAIVVSRFNELICKKLLEGALDCIKRHGG
                     DLDQVTIAWVPGAFEIPVAAKRIARSTQYDAVICLGAVIRGATPHFEYIAAEVTKGIA
                     QAGMELEVPITYGVITPDNLEQALERAGTKAGNKGWDAAMSAIEMADLFAHLKG"
     gene            1530..3701
                     /locus_tag="PKM23_11245"
     CDS             1530..3701
                     /locus_tag="PKM23_11245"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNY92088.1"
                     /translation="MRRVVGPIPVLILLLCTLAPLRAAEWEWRTFTNASDVRQVLAAD
                     TLVWAATNGGVLSYSPVTGRFTPFVNTDGLAGNEVAAVERDRHGRIWMAASGGLINVY
                     TPSSGRWQTISDYDGQSIKDLTVLGDSLFVSLDIGISLFDAGRWEVKETYKIGRVNRT
                     LIDGRQIWAACPDGVRASNLDLPNLMAPAAWTLYTIADGLPHSEALSLAAHEGRIYTG
                     TLKGLGIFDGTSWLPGELLDNTIADLAVWGEQLAVSAGYAVLRRNGANSYSQIGNGLA
                     QVTSLSVDGSGRLWAGISQNGLAWIGPEGTAWNRLLPDGPADNKYTALTFDRNGVLWT
                     ASSSGGISRYDGSSWRVFNRKNGLLPGNDYRTLAVDNRNRVWAGSWGTGIALFTTSGD
                     SIAIDILDAQDGLAGISVNPNYVVITGLHADKNGNMWILNYQADNLQILAVVDSLGNW
                     QYFTTRDGIKSKLTTAIASDQNNRVWIGTQDRGISVLDHNDTPFNKSDDDLSQGLSTE
                     DGLNSLAIRTLACDADGVMWIGTPEGLEYWYEGALNPRYQVINDDINVVSVDVRNNKW
                     IGTSGGMSMFEADGYTWRHFSTSTSALVGDNVTSFAFNEHTGQLYIGTTNGLSRLQTP
                     FTRPAANLSRLAGYPNPFTLEGPGALFYIENLADKATVRIYTPEGRLIRHIPAVQIHG
                     SRTIWDGRNDRGELVASGIYLFLATTESGEAGSGKVAVIRP"
     gene            3895..>5284
                     /locus_tag="PKM23_11250"
     CDS             3895..>5284
                     /locus_tag="PKM23_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNY92089.1"
                     /translation="MLTFLYAKNYLLIDELKVGFTPGLNIITGETGAGKSILIGALGA
                     VLGDRLGKEVIRGGSDKAVIEAEFLVSSLPAAAAFLREKEIEEQGESLLLRREVHATG
                     KSRCFINDTPVPLPVLTELGDLLVDLHGQHEHQLLLRVANHGPYLDAFAGLESDLEAL
                     REAYQELTAATRELRAVEEQAAELARSRDYMAFQLQEIRAVAPAADEEEELLREEQIL
                     RNAELLFERTAQIFTRLYESEGSAAEQLTAAAADLAQLAAIDSRFSAMQGDCQSARIL
                     VDELAKSVQHYNQAIAFDPERLEKIRQRLAALTALRRKHGGSLADIIALGDRLERDLG
                     LVDNLEATLARLGREREAARSRLAGINAGLSGKRHGAAEDFSSRVAAALALLGMPRAQ
                     FAVAQHSTPAADEPWVELEGQRVRIGARGSDHLEFLLSANPGEEAKPLAAVASGGEIS
                     RVMLALKTLLAEA"
BASE COUNT         1009 a         1821 c         1572 g          882 t
ORIGIN      
        1 ccgcctttac ggtctcggtc gactaccgct acggcggggt gaccaccggc atctcggccg
       61 ccgaccgcgc cgccaccatt ctcgccctca ccgatccgca ggtcaaaccc gccgattttg
      121 ccaagccggg gcatatcttt cccatcgctg cggttaccgg gggggtgctg cgccgcgctg
      181 gccacaccga ggcggtggtc gacctcgccc gcgtcgccgg ggcctatccg gctggcgtcc
      241 tctgcgagat catggacgag gacggcagca tggcgcgcct gcccgccctc ttcaagttcg
      301 cccgccagtt cgacctgcgc atcatcaccg tccgcgccat gatcgaatac cgccgccgta
      361 acgagattct catcaaccgc gtcgccagca ccgattttcc gacccgcttc ggtcactttg
      421 ccctgcatgt ctacgaaagc aaggtcgacg accatcatca cctcgccctg gtcaagggtg
      481 atgtcgccgg cgaggagccg gcgctggtcc gcgtccattc gcaatgcctc accggtgacg
      541 tcttcggctc gatgcgctgc gattgcgggg aacaattgga atatgccctg cgtgaaatcg
      601 aaaagaaggg tcgtggcgtc ctcctctata tgcgccagga gggacgcggc atcggtctgg
      661 ccaaaaagat catggcctat gccctgcagg atcagggcaa ggacaccgtc gaggccaacg
      721 aggccctcgg ctacaaggcc gacctgcgcg actatggcat cggcgcccag atcctctggg
      781 acctcggggt gcgcaagatc gcgctgctga ccaacaaccc caaaaagatc atcggcctca
      841 agggctacgg cctcgaggtg gtggagcgcg ttccgatcga ggtcgagccc aacagcgtca
      901 acggctttta cctcgagacc aagcgcgaca agctcggaca tctcatcatg atggacgagg
      961 aaggcaagca accagacgtc caggaaagct gaagaggagc gctcaacgct cctgtgaaat
     1021 cccgataccc tgaccattaa cccaggtgaa tcccatgcca cacgtgattg aaggccaact
     1081 ttcggcgagc ggaaaaaagt tcgccatcgt cgtcagccgc ttcaatgaac tgatctgcaa
     1141 aaagctcctc gagggcgcgc tcgactgcat caaacgccac ggcggcgacc tcgaccaggt
     1201 caccatcgcc tgggtgcccg gggcgttcga aatcccggtc gcggccaagc gcattgcccg
     1261 cagcacacag tacgatgcgg tgatctgcct cggagcggtc atccgcgggg cgaccccgca
     1321 ctttgaatat atcgccgccg aggtgaccaa gggcatcgcc caggccggca tggagctgga
     1381 ggtgccgatc acctacggcg tcatcacccc ggacaacctc gagcaggccc tcgagcgcgc
     1441 cggcaccaag gccggcaaca agggctggga tgcagcgatg agcgccatcg agatggccga
     1501 cctttttgcc catctcaagg ggtagccgga tgagaagagt ggtgggaccg atccccgttc
     1561 ttatactgct gctttgcacc ctcgcacccc tgcgcgcggc cgagtgggag tggcgcacct
     1621 tcaccaacgc ctccgatgtg cgccaggtcc tggccgccga caccctggtc tgggccgcca
     1681 ccaacggcgg agtgctcagc tacagccctg tgaccggccg cttcaccccc tttgtcaaca
     1741 ccgacggcct tgccggcaat gaggtcgccg ccgtcgaacg cgaccggcac ggccgcatct
     1801 ggatggcggc cagcggcggc ctgattaatg tctatacccc ctcctccggg cgctggcaga
     1861 ccatctccga ttatgacggc cagagcatca aggacctgac cgtgctcggc gattccctct
     1921 tcgtctctct ggatatcggc atcagcctct tcgacgccgg ccgctgggag gtcaaggaga
     1981 cctacaagat cggccgcgtc aaccgcaccc tcatcgacgg ccggcagatc tgggcagcct
     2041 gtccggacgg cgtgcgcgcc tccaacctcg atctccccaa tctcatggcc ccggctgcct
     2101 ggaccctcta taccatcgcc gacggcctgc cccacagcga ggccctctct ctggccgccc
     2161 atgaaggccg catctatacc ggcaccctca aggggctggg catcttcgac ggcacctcct
     2221 ggctgcccgg cgaactgctc gacaacacca tcgccgacct ggcggtgtgg ggggagcagc
     2281 tggcggtatc cgccggctac gccgtcctca ggaggaacgg ggcgaacagc tacagccaga
     2341 tcggcaatgg tctcgcccag gtcacctccc tcagcgtcga cggcagcggc cgcctctggg
     2401 cggggatcag ccagaacggc ctcgcctgga tcggcccgga gggcaccgcc tggaacagac
     2461 tcctgcccga cggcccggcg gacaacaaat ataccgccct cacctttgac cggaacgggg
     2521 ttctctggac tgcctcctcc agcggcggca tctcccgcta cgacggcagc tcctggcgcg
     2581 tcttcaaccg caagaacggc ctgctccccg gcaacgacta tcgcactctc gccgtcgaca
     2641 accgcaaccg ggtttgggcc ggaagctggg gaaccggaat cgccctcttc accaccagcg
     2701 gcgacagcat cgccatcgac atcctcgatg cccaggacgg cctggccggc atctccgtca
     2761 atcccaatta tgtggtcatc accggtctgc atgcggataa aaacggcaat atgtggattc
     2821 tcaactacca ggccgacaat ctgcagatcc tcgccgtcgt cgactccctc ggcaactggc
     2881 agtatttcac cacccgcgac ggcatcaaat cgaaactgac cacggcgatc gcctccgacc
     2941 agaacaaccg ggtctggatc ggcacccagg accgcggcat cagtgtcctc gatcacaacg
     3001 ataccccctt caacaagtcg gatgacgacc tcagccaggg gctgagcacc gaagacggcc
     3061 tcaacagcct cgccatccgc accctcgcct gcgatgccga cggagtgatg tggatcggca
     3121 ccccggaagg gctggagtac tggtacgagg gcgctctcaa tccgcgctat caggtcatca
     3181 atgacgacat caatgtcgtc tccgtcgatg tgcgcaacaa caaatggatc ggcaccagcg
     3241 gcggcatgag catgttcgag gccgacggct acacctggcg ccatttctcc acttccacca
     3301 gcgccctggt cggcgataac gtcacctcct tcgccttcaa cgagcacacc ggacagctct
     3361 atatcggcac caccaacggc ctctcgcggc tgcagacccc tttcacccgg ccggcggcca
     3421 atctgagcag gctcgcggga tatcccaatc ccttcacgct ggagggcccg ggtgcgcttt
     3481 tctatatcga gaacctggcc gataaggcca ctgtacgcat ctacaccccg gaaggacgcc
     3541 ttattcggca tatccccgcc gtacagatcc atggctcgcg cacaatctgg gacggccgca
     3601 atgaccgcgg cgaactggtc gccagcggca tctatctttt cctggccacc accgagtcgg
     3661 gcgaggccgg atcgggcaaa gtggcggtca tccgcccctg agcaattcaa attaacttgt
     3721 ccgttattac aattttccaa aaatccgctt gataatatct cgccaattcc gttaatttag
     3781 agtagttaca ggagcgaacc gggttgggga ggagactatc agtcgcgccg gcggtgcgcg
     3841 gggcaaaggg ataaggcctc agatgcggcg ccggaaaagg tttttaacaa gagcatgctc
     3901 acttttctct acgcgaaaaa ctatctcctc atcgatgaac tcaaggttgg cttcacgccg
     3961 ggactgaata tcatcaccgg cgagaccggc gcgggcaaat cgatcctcat cggcgccctc
     4021 ggcgctgtgc tgggagaccg cctcggcaaa gaggtgatcc gcggcgggtc cgacaaggcg
     4081 gtgatcgagg ccgaattcct cgtctcttcg ctgccggcgg ccgcggcctt tctgcgcgag
     4141 aaggagatcg aggagcaggg cgaaagcctc ctgctgcgcc gtgaggtgca tgccaccggc
     4201 aagagccgat gcttcatcaa cgacaccccg gtacccctgc cggttctgac cgaactgggc
     4261 gatctgctcg tcgatctgca cggccagcat gagcatcagc tcctcctcag ggtcgccaac
     4321 cacggcccct atctggacgc tttcgccggg ctggagtccg atctggaggc cctcagggag
     4381 gcctatcagg aactgaccgc cgcgacgcgg gaactgcgcg ccgtggagga gcaggctgcc
     4441 gagttggccc gctcccgcga ttacatggcc tttcagctcc aggagatccg ggcggtggcc
     4501 ccggcggccg atgaagagga ggagctgctg cgcgaggagc agatcctgcg caatgccgaa
     4561 ctgctcttcg agcgcaccgc gcaaattttc acccggctct acgagtcgga gggctcggct
     4621 gccgaacagc tgaccgcagc cgcggccgat ctcgcccagc tggcagccat cgacagccgc
     4681 ttcagcgcca tgcagggcga ttgccagagc gcgcgtatcc tcgtcgacga gctggccaag
     4741 agcgtacagc actacaatca ggccatcgcc ttcgatccgg agcggctcga aaaaatccgt
     4801 cagcgtctgg cggccctgac cgccctcagg cgcaagcatg gcggcagcct ggccgacatc
     4861 atcgcccttg gcgacaggct ggagcgggat ctggggctgg tcgacaacct cgaggcgacc
     4921 ctcgcccggc tgggcaggga acgagaagcg gcgcgcagcc ggctggccgg gatcaatgcc
     4981 gggctctccg gcaagcgcca tggtgccgcg gaggatttca gcagccgggt ggccgccgcc
     5041 ctcgcgcttc tcggcatgcc gcgcgcgcag ttcgcggttg cccaacactc tacgcccgcc
     5101 gctgatgagc cctgggtcga gctggagggg cagcgggtcc gcatcggagc gcgcggctcc
     5161 gaccacctcg agttcctgct ctccgccaat ccgggtgagg aggccaagcc cctggcggcg
     5221 gtggcctcag gcggcgagat ttcgcgcgtc atgctcgccc tcaagaccct gctcgccgaa
     5281 gccg
//
LOCUS       DAOPWU010000241         5277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15647_length_5277_cov_5.373229, whole genome shotgun sequence.
ACCESSION   DAOPWU010000241 DAOPWU010000000
VERSION     DAOPWU010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15647_length_5277_cov_5.373229"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1299)
                     /locus_tag="PKM23_11255"
     CDS             complement(76..1299)
                     /locus_tag="PKM23_11255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNY92090.1"
                     /translation="MTASGDPQGLIPAAARDALILSACTEIIKQGRHADAVLQRALRT
                     DPAFTLEEKSAIATAVFDIVRFARLLGAITSGDPDAPPVLNAAALEAWRLWRGQHPPA
                     GPAEHEMLERAARFSAVRTLRESLPDWLDQLGAAGRGERWESLLAALNRPPQQVLRAN
                     TLRTTTAALLAEAARMGYPAHPVEWAPAAVVLEGHAPVFQWECFRKGWFEVQDAASQT
                     VAVFMEVKPGMRVVDGCAGHGGKTLHLAALMENRGRIIALDTVEEKLAELVRRARRAG
                     AQIIEARRITSTKIIKRLAGSADRLLLDVPCSGTGVWRRNPDAKWHLQPEELTRLQAN
                     QQQILSYYSRIVKPGGRLVYSTCSVLPAEGEEQVARFLTAAGNAFSLAAEQRLDPDTH
                     GFDGFYMAALERTSS"
     gene            complement(1296..1988)
                     /locus_tag="PKM23_11260"
     CDS             complement(1296..1988)
                     /locus_tag="PKM23_11260"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNY92091.1"
                     /translation="MTPLHEGERPIVVCPYLAREIELNGRLEDPLWQRAAAIELVDAV
                     SGRPGRFSTSVRLAWSSTCLYAAFICEDDYVWGDIAEHDGLIYDQECVEIFINPSGCP
                     HTYFEINLSPKNVVFDACILNDRTAANPDGPFTGLHCWEAAGLRTAVSIQGEVDTPGA
                     ARGWIAEYALPFRALYGASHQPPLPGDLWRVNFYRIDSPRRGEQELYAWNPVLRPTFH
                     LPWRFGSLRFGA"
     gene            complement(2040..2906)
                     /locus_tag="PKM23_11265"
     CDS             complement(2040..2906)
                     /locus_tag="PKM23_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92092.1"
                     /translation="MIARTLHSALTRLFALKRLWLVYYLSGLAFAAVVALPATSIVSS
                     YAGQTLTAERLSSFMDINFIIELFHYHGSALGAILPLLIMTAFLFWLLMLFFSGGALK
                     LMAGDHPYRPREFWDACGRYFGRFLRLLLWSLPLLALFFLLPALANLVEKLFWGKDPG
                     DNITYWSGIAKMGLRYLALMLWALFFDYARILAVQKNEKRTRRLAIAALQFTSRNLGR
                     LFALALLLALISLLGLVIYNPVADLLHAPSTGIIALLFLWQQLYMLFRAGVRLLTHGS
                     QMKLSQRLLQDY"
     gene            complement(2927..4999)
                     /locus_tag="PKM23_11270"
     CDS             complement(2927..4999)
                     /locus_tag="PKM23_11270"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNY92093.1"
                     /translation="MALPRTACRMFCLSVVLITLRTAAGQPVLFPEPKSPRIANYDID
                     VRLDTEARLLHGRQVLRWRNTASHAVEELQFHLYLNGFRNDQSTFMLESGGRHRGEKL
                     DEKGWGNIQIDSLTNAAGEELLPMTSFIQPDDGNINDKTVLRLLLPRPLPPGETITLH
                     FAFTAKLPEPPMARTGIRKEFVMAGQWFPKIGVYEEGAWNCHQFHANSEFYADFGVYN
                     VDITVPRGHIVGATGICYATVDNSDSTTTHRFHAEDVHDFAWTASSDFIVLNGKSQDV
                     EIRTLIQKDHASMGARYLKAAEIAVAWFQDQYGDYPYPNLTVVDPRRGASGAGGMEYP
                     TLITAWGAYGLPEGVRALEMVIMHEFGHNYWYHLVASNEFEEAWLDEGINTFSEMQIL
                     ADTYGEAGSMIDLWGLRISDTPIQRMSYIAQPGLDPVLQPAWKYYSGNNYASMSYNKP
                     GLMLLTLQNLVGHETMNRILRTWFERWQFKHPRSTDFIAIANEISGQNLDWFFDQALR
                     STAVLDYSVSEIGSREVKKASGFDFFPADSAAAGPSADTLAAGGSAPDTAAMGEKADP
                     DSGQKAAQEKKLYTSTVKVRRLGEFVFPVELQMIFADGDTVNEKWDGVERWHKFSVTR
                     PARLLSAAIDPENKILLDVQLTNNSRTLESSSRGINKLTARILFWSQFIMDMPGVLNL
                     FTAFSPNL"
BASE COUNT          968 a         1446 c         1891 g          972 t
ORIGIN      
        1 tatgggagca ggaggctgca cggagcggcc gcggcggcga agcgataggc tgattcggat
       61 ggcccgggcg ccagctcagg acgaggtgcg ctccagggcc gccatataaa aaccgtcgaa
      121 tccgtgggtg tccggatcga ggcgctgctc agccgcgagg gagaaggcgt tgccggcggc
      181 ggtgagaaag cgggccacct gttcttcgcc ctcggcgggc agaacactgc aggtggagta
      241 gacgaggcgg ccgcccggct tgacgatgcg gctgtagtag ctgaggatct gctgctgatt
      301 ggcctgcagc cgggtcagtt cctcaggctg caggtgccat ttggcgtccg gattgcgccg
      361 ccagacccct gtaccggagc aggggacatc gagcagaagg cggtcggccg aaccggcgag
      421 gcgtttgatg atcttggtcg aggtgatcct tctggcctcg atgatctggg cgccggcgcg
      481 gcgggcgcgg cgcaccagct ctgccagctt ttcttccacg gtatccaggg cgatgatccg
      541 gccccggttt tccatcaggg cggcgagatg gagggtcttg ccgccgtgac cggcgcagcc
      601 gtcgaccacc cgcatgccgg gcttgacctc catgaacacc gccactgtct gggaggcggc
      661 atcctgcact tcaaaccatc cctttctgaa acattcccat tgaaaaaccg gggcgtgccc
      721 ctcgagcacg accgccgccg gggcccactc caccgggtga gcggggtatc ccatgcgggc
      781 ggcctctgcg agcagggctg cggtggtggt ccggagggtg ttggcgcgca gaacctgctg
      841 cggcgggcga ttcagcgccg cgagcagact ttcccagcgc tcgccgcgcc cggccgctcc
      901 gagctgatcc agccagtcgg gaagggactc gcgcagggtg cggacggcgg agaatcgggc
      961 cgcccgctcg agcatctcgt gttcggcagg gcccgcgggc gggtgctggc cgcgccagag
     1021 cctccaggcc tccagcgcag ccgcgttgag aacgggggga gcatccggat cgccgctggt
     1081 tatggcgccg agtaagcggg caaagcggac gatgtcgaag accgccgtgg cgatagcgct
     1141 tttctcttcc agggtgaagg cgggatctgt gcggagggcg cgctgcagga cggcgtcggc
     1201 atggcggccc tgtttgatga tctcggtgca ggcggaaagg atgagggcgt cgcgggctgc
     1261 ggccggaatc agcccctggg gatcaccgct ggcagtcatg cgccgaaccg cagtgagccg
     1321 aaacgccagg ggagatggaa ggtggggcgc aggaccggat tccaggcgta gagctcctgc
     1381 tctccacggc ggggggaatc gatgcgataa aaattgacgc gccagaggtc gccgggcagg
     1441 ggcggttggt gggatgctcc gtagagggcc ctgaagggga gggcgtactc ggcgatccag
     1501 ccgcgtgcgg ctccgggtgt atccacctct ccctggatcg aaaccgcagt gcgcagccct
     1561 gccgcctccc agcagtgcag tcctgtgaag gggccgtccg ggtttgcggc ggtgcgatcg
     1621 ttgaggatgc aggcgtcgaa gacgacattt ttcgggctga ggttgatctc gaaataggta
     1681 tgggggcagc ccgagggatt gataaaaatc tcgacgcact cctggtcgta gatcagcccg
     1741 tcatgctccg caatatcccc ccagacgtaa tcgtcttcgc agataaaggc agcgtagagg
     1801 caggtgctgc tccaggccag gcggaccgag gtgctgaatc gtcccggccg gccgctgacg
     1861 gcgtccacca gttcgatggc ggctgcgcgc tgccagaggg ggtcctccag gcggccgttg
     1921 agttcgatct cgcgagctaa gtacgggcag accacgatcg gccgctcacc ttcgtgcagt
     1981 ggtgtcactc ctttcctcct ttaatgttgc cattctcttg cgcaccctct cctccgcgat
     2041 cagtaatcct ggagcagcct ttgactgagc ttcatctggc tgccgtgggt gagaaggcgc
     2101 actccggcgc ggaagagcat gtagagctgc tgccagagga agagcagggc gatgattcca
     2161 gtcgaggggg cgtggagcag gtccgccacc ggattataga tcaccagtcc gagcaggctg
     2221 atgagggcga gcagcagggc cagggcgaag aggcggccga ggttgcggct ggtgaactgg
     2281 agagcggcga tggccaggcg gcgcgtccgt ttttcgtttt tctggacggc gaggatgcgg
     2341 gcataatcga agaagagggc ccagagcatc agggcaagat agcggagtcc catcttggct
     2401 atgccgctcc agtaggtgat gttgtcgccg ggatccttgc cccagaagag tttctcgacg
     2461 aggttggcca gggcaggcag caggaagaag agggccagca gggggaggct ccagagcaag
     2521 aggcggagga agcggccgaa atagcggccg caagcatccc agaattcgcg cggccggtag
     2581 ggatgatcgc cggccatgag cttgagcgca ccgccggaaa aaaagagcat cagcagccag
     2641 aagaggaagg ctgtcattat gaggaggggc agtatggcgc cgagggcgct gccgtggtag
     2701 tggaacagtt cgatgatgaa attgatatcc ataaaactgc tgaggcgctc ggcggtgagg
     2761 gtctggccgg cgtagctgga gacgatcgag gtcgccggca gggcaacaac agcggcgaaa
     2821 gccaatccac tgagataata gacgagccag agccgcttga gcgcgaagag gcgagtcagg
     2881 gctgaatgta gtgtgcgtgc aatcatcagg cgttcctcgc gagcggttac aggttgggtg
     2941 agaaggcggt gaagaggttg agaactccag gcatgtccat gataaactgg ctccagaaga
     3001 gaatgcgcgc ggtgagtttg ttgataccgc gggaggaact ctccagggtg cggctgttgt
     3061 tggtgagctg gacgtcgagg agtatcttgt tctccggatc gattgcggcg gaaagcagac
     3121 gggcgggccg ggtgacggag aatttatgcc agcgttccac gccgtcccat ttttcgttca
     3181 ctgtatcgcc gtcggcgaag atcatctgca gctcgaccgg gaagacgaat tctccgaggc
     3241 ggcgcacctt cacggtcgag gtgtagagtt tcttttcctg ggcagccttc tggccgctgt
     3301 cagggtctgc cttttcgccc atggcagccg tatccggagc ggagccgccg gcagccaggg
     3361 tatccgcgga gggtccggcg gcggccgaat cagcggggaa gaaatcaaat ccgctggcct
     3421 tttttacctc cctgctgccg atctcgctga cgctgtaatc gagcaccgcg gtggaacgga
     3481 gggcctggtc gaagaaccag tcaagattct gaccagagat ctcgttggca atggcgataa
     3541 aatcggtgct gcgcggatgt ttgaactgcc agcgctcgaa ccaggtgcgc aggatgcggt
     3601 tcatggtctc gtggccgacc aggttttgca gggtgagcag catcaatccg ggtttgttgt
     3661 aagacatcga ggcgtagttg tttcccgaat agtatttcca ggcgggctga aggacgggat
     3721 cgaggccggg ctgggcgatg tagctcatgc gctgaatggg ggtatcgctg atgcggaggc
     3781 cccagagatc gatcatgctg ccggcctcgc cgtaggtgtc ggcgagaatc tgcatttcgc
     3841 tgaaggtgtt gatgccctcg tcgagccagg cctcctcgaa ttcattggag gcgacgaggt
     3901 ggtaccagta attgtggccg aactcgtgca tgatcaccat ctcgagggca cgaacgccct
     3961 ccgggaggcc ataggcgccc caggcggtga tcagggtcgg gtactccatg ccgcccgccc
     4021 cgctggcgcc gcggcgggga tcgacgacgg tgaggttggg atagggatag tcgccgtact
     4081 gatcctggaa ccaggcgaca gcgatctcgg ccgccttgag gtagcgcgct cccatggagg
     4141 cgtggtcttt ctggatgagg gtgcggatct ccacatcctg gcttttaccg ttgaggacga
     4201 tgaaatcgga actggccgtc caggcgaaat catggacatc ctcggcgtgg aagcggtggg
     4261 tggtggtgga gtcggaatta tccaccgtcg catagcagat gccggtggcg ccgacgatgt
     4321 ggccccgggg cacagtgatg tcgacgttgt agaccccaaa atcggcatag aactcggagt
     4381 tggcgtgaaa ctggtggcag ttccaggcgc cctcttcata gactccgatc ttggggaacc
     4441 actggccggc catgacgaac tcttttctga tgccggtgcg ggccatcggg ggctcgggca
     4501 gcttggcggt gaaggcgaaa tgaagtgtga tggtctcacc ggggggaagg ggcctgggga
     4561 gcagcagccg gagaacggtt ttatcattga tattgccgtc gtcgggctgg atgaaggagg
     4621 tcatcgggag cagctcctcc ccggctgcat tggtcaggga gtcgatctgg atattgcccc
     4681 agcctttctc atcgagcttt tcgccgcggt ggcggccgcc cgattcgagc atgaaagtcg
     4741 actggtcgtt gcggaagccg ttgagataga ggtgaaactg tagttcctcg acggcgtggc
     4801 tggcggtgtt gcgccagcgc agaacctggc ggccgtgcag aaggcgggcc tcggtgtcga
     4861 ggcggacatc gatgtcatag ttggcgatgc gcgggctttt aggctcgggg aagagcacgg
     4921 gctggccggc ggccgtcctg agcgtgatca gcacaacgga aaggcagaac atcctgcagg
     4981 cggtacgggg aagggccatt ctcgtctccg gttttatcag agtggtatat gctgggcagg
     5041 ttgagtcccg acctcttgtt ctatacgaat gaaaccgctc cgggtttcgc ccggcggccc
     5101 ggcctctgct tgtcagcgga gcaggaccag cttgcggacc agctgttctc catcgctttg
     5161 cagccggtag agataggttc cgccggcgac cgatcttccc tgattatcgc ggccatccca
     5221 gaggagccgg tggcggcccg tcggcagggt ggtctcggcg aggcgcctga ccaggcg
//
LOCUS       DAOPWU010000242         5255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15747_length_5255_cov_4.182885, whole genome shotgun sequence.
ACCESSION   DAOPWU010000242 DAOPWU010000000
VERSION     DAOPWU010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15747_length_5255_cov_4.182885"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKM23_11275"
     CDS             complement(<1..324)
                     /locus_tag="PKM23_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92094.1"
                     /translation="MAGWLLLLIFLMMTGGSWAQTMTEQSHKRGKLWENISSDGWIGS
                     LGAWDFLVSMPLGLYPGFKDYYHPVGNENKAVNTYANANFHNFRSGCWIVAKDLMTPG
                     PPPKNA"
     gene            complement(369..3206)
                     /locus_tag="PKM23_11280"
     CDS             complement(369..3206)
                     /locus_tag="PKM23_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY92095.1"
                     /translation="MNWWPFKNEVLLVLLLLQGILLSPGALHAATWGKIAGRITDGRT
                     KEPLLGANVIVTGTNLGAVTDLSGDYYIANIATGTYTLKVTMVGFKSVTIKEVQVRIN
                     RTTEVNFTLEQSVLEGEDIVIIAERPLIEKDNTSSNMILASQEIISRPTTEFMDVLTT
                     LPSINLENGELKFRGGTLDQVAFMIDGTRAQNPMNHSPYTNINLSSIAEVEVITGSFN
                     AEYGEAMSGVVNVVTKEGSDRYSLFIDGRYTPPGKKHWGDALYDLNSDLYWENSHARH
                     PEWWIENTDQWVDPSGTYGYDPGCLWTPEQAFQDYLRTHRPLTDYTKRAGYQTEISVG
                     GPIPLVKNLSFYATGKIRSQAPLMGNAYRDEGRFFDGTLKLSYQLAPSMKLVLSGFGG
                     KEESCWGMEWVDSWYTQRYGLNSRYAYYDFAGLPNNSTDGQTLTFHHVLSPKTMYEIK
                     LNRVHAKRKTWTFPEDSVGWESTEAIYDFLRATDENGYSIQGGYMNAIGYHTLGYYYR
                     YDDDNTEYNLTGFLGSTINKYWYLKTGVDLSYYNLDHFNQAKAPNRRDDRTYNPYQGA
                     LYFQNKLEFGGLIMNAGLRFDFYNPNDYVYTNIFDPLNAEAKKTKIFTQLSPRLGISH
                     PIDERTVLHFSYGHFFQRSSFGDYGEGNVSGDQQGSLTTFIIDGSNFPWVLGNREVRP
                     LKTIAYEVGLERNFADQFVVDITGYYKDIRNTIRTTTIESPFGVYTTNGNGNYADVRG
                     VELSLRKLPLVGRFGAVWGYANFTHQIGIYGSSGDPDVIRGDGSVRYPASGDVIAHNN
                     PRVKAGFSYQTPDGSSFIGKILQDLTMSVNFEAIFPNEKIRSDYFLYDGVRYMRPADK
                     HTSLRVKKEISMPGIGLRVNPYVEVHNLFNEKWLNLATFENASYDDQARFASSGFSYI
                     PAFDMTGAPILAAAQYRNLPRSIMFGATFEF"
     gene            complement(3251..4201)
                     /locus_tag="PKM23_11285"
     CDS             complement(3251..4201)
                     /locus_tag="PKM23_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNY92096.1"
                     /translation="MKAAILHRPGKFVIEERPIPSITPDQVLVKTTACGVCTGEVEMW
                     EGKNPGIKFPIFIGHEASGIIEKVGANVREYQAGDHVAAWIEGQGYADYFAVHFGSLY
                     RLRPETPMELALGEPIACAVNGVRKADVQFNQSVCLVGCGFMGLIMLQVFRARGAGMI
                     IAVDTREEILQLAQQLGAAYTFNPRTADVAQAVRDLTGGKGVDIGVEAAGRQETLDLT
                     AALVRMEGKLEVFGFHQGGPRQVDWGYWNWMAFQIVNGHSRSAHLYIEGMNIGLELME
                     AKKLVMKPLVTHRFPLFEINEAFRTAAGKSPGFIKGVIVF"
     gene            complement(4333..>5255)
                     /locus_tag="PKM23_11290"
     CDS             complement(4333..>5255)
                     /locus_tag="PKM23_11290"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY92097.1"
                     /translation="IEANAKLLWSNDWLYVYAKVEDYEFFFGPDTANWIGEQIMVGID
                     GPMYGDPAGGNYADDWSGAPWNAPDKGPTVYKINDKGITSNWGGWWDDPNQTWWEVLP
                     VDTSWVVGQVFVNNEDLTWGVEMKIYVPQIALGTRIGFNIGGAAATPDTTAVPWEWGE
                     GTYAYYAWHPQPGDTAVGYPSDYIAGDIQRRAKCFGMLTFKQSITGVNDYELERGSLP
                     EMFTLTQNYPNPFNPSTTLTYRLQKDARVCIDVYNVLGKKVGNLVERRQAAGTYSVKW
                     DAGDLASGIYFFQLRVNDQLISSRKAMLLK"
BASE COUNT         1051 a         1452 c         1623 g         1129 t
ORIGIN      
        1 cgcattcttg ggaggcggcc ccggggtcat caaatccttg gcgacgatcc agcatccgct
       61 gcggaagttg tgaaaattgg cgttggcgta ggtgttgacc gccttgttct cattgccgac
      121 ggggtgataa tagtccttga atcccggata gaggccgagg ggcatggaga ccagaaaatc
      181 ccaggccccg aggctgccaa tccagccgtc actgctgatg ttctcccata atttgccgcg
      241 tttgtgggat tgttcggtca tggtctgcgc ccagctgccg ccagtcatca tcaggaagat
      301 gagcagtagc agccatccgg ccatgctttg gataacatgc gctttcttca tagcatctcc
      361 atggcaaact agaattcgaa tgtcgcaccg aacatgattg agcgcggcag attgcggtac
      421 tgcgccgcag ccaggatggg cgccccggtc atgtcgaacg cgggaatata gctgaatccg
      481 ctcgaggcga acctggcctg atcatcatag gaggcgttct caaaggtcgc cagattgagc
      541 catttctcat tgaaaaggtt gtgcacctcg acgtagggat tgacgcgcag gcctatgccg
      601 ggcatggaaa tctccttctt gacgcggagc gaggtgtgct tgtcggccgg gcgcatgtag
      661 cgcacgccgt cgtaaagaaa ataatccgac ctgatcttct cattgggaaa gatcgcctca
      721 aagttgaccg acatggtgag atcctgcagg atcttgccga tgaacgatga gccgtccggg
      781 gtctggtaag aaaagcccgc cttcacgcgc ggattgttgt gagcgatgac gtccccgctg
      841 gcgggatagc gcacgctgcc gtcgccgcgg atgacatcag gatcaccgct gctgccgtaa
      901 ataccgatct gatgggtaaa attggcataa ccccaaaccg ccccgaagcg cccgaccaat
      961 ggcagtttgc gcaaggagag ctcgacgccc ctgacatctg catagttgcc gttgccgttg
     1021 gtcgtataga cgccgaatgg cgattcgatc gtggtcgtgc ggatggtatt gcggatatcc
     1081 ttgtaatagc cggtgatatc caccacgaac tggtcggcaa agttacgctc gaggccgacc
     1141 tcataggcga tggtcttgag tggccgcacc tcgcggttgc ccagcaccca gggaaagttg
     1201 gaaccgtcaa tgatgaaggt ggtcagactc ccctgctggt cgcccgaaac attgccctcg
     1261 ccatagtcgc cgaaggagct gcgctggaag aaatgcccat aggagaaatg gagaaccgtg
     1321 cgctcgtcga tgggatgcga gatgcccagg cgtggactga gctgggtgaa gattttagtc
     1381 tttttcgctt cggcattgag gggatcaaag atattggtat acacatagtc attgggattg
     1441 tagaaatcga agcgcaggcc ggcgttcatg atcaggccgc cgaattccag cttgttctgg
     1501 aaatagaggg ctccctgata gggattatag gtccggtcat cccgccggtt gggggccttg
     1561 gcctggttga aatggtcgag gttgtagtag gagaggtcga cgccggtctt gagataccag
     1621 tatttgttga tggtgctgcc gagaaaaccg gtgaggttgt attcggtgtt gtcgtcgtcg
     1681 tagcggtaat agtagccgag ggtgtgatag ccgatggcat tcatatatcc gccctggatg
     1741 gaatagccat tctcatctgt ggcgcgcaaa aagtcataga tggcctcggt tgactcccac
     1801 cccaccgagt cctcaggaaa ggtccaggtc ttgcgtttgg catggacgcg gttgagcttg
     1861 atctcgtaca tggttttggg actgaggaca tgatgaaaag tgagggtttg gccgtcggtg
     1921 ctgttgttcg gcagcccggc gaaatcgtaa taggcatagc gcgaattcag tccgtagcgc
     1981 tgggtgtacc aggaatccac ccactccatg ccccaacagc tctcctcttt gccgccgaaa
     2041 ccggagagga ccagtttcat tgagggggcc agctgatagc tcagcttgag ggtgccgtcg
     2101 aagaagcgac cctcgtcgcg gtaggcattg cccatgaggg gggcctggga gcggatcttg
     2161 ccggtggcgt aaaagctgag gtttttaacg agggggatgg gaccacccac gctgatctcg
     2221 gtctggtagc cagcccgctt ggtatagtcg gtgaggggcc gatgggtgcg gagataatcc
     2281 tggaaggcct gctccggggt ccagaggcag ccgggatcat atccgtaggt gccagaggga
     2341 tcgacccact ggtcggtatt ctcgatccac cattcgggat ggcgggcgtg gctgttctcc
     2401 cagtaaagat ctgagttcag gtcatagagg gcatcccccc agtgcttctt gccggggggg
     2461 gtgtagcggc cgtcgatgaa gagggagtag cggtccgatc cctccttggt gacgacgttg
     2521 accacgcctg acatcgcctc gccatactcg gcattgaatg agccggtaat aacctcaacc
     2581 tcggcaatgg agctgaggtt gatgttggtg taggggctgt ggttcatcgg attctgcgcc
     2641 cgcgtgccat cgatcataaa ggccacctgg tccagggtgc cgccgcggaa cttgagctcg
     2701 ccgttctcga ggttgatgct gggcagcgtg gtcaggacgt ccatgaattc cgtggtcggg
     2761 cgggagatga tctcctggct ggcgaggatc atgttggagg aggtattgtc cttttcgatg
     2821 agggggcgtt cggcaatgat gacgatgtcc tcaccctcca ggaccgactg ctcgagggtg
     2881 aaattgacct cggtggtgcg gttgatgcgc acctggacct ccttgatggt cacgctcttg
     2941 aagcccacca tcgtcacctt gagggtgtag gtcccggtgg cgatgttggc gatgtagtaa
     3001 tccccggaga ggtcggtgac cgctccgagg ttcgtcccgg tgacaatgac gttggcccca
     3061 agcaggggtt ccttcgtcct gccatcagta atcctgccgg caatcttgcc ccaggtcgca
     3121 gcatggagtg cgccgggact gagaagaatc ccttgcagca acaggagaac aagcaggacc
     3181 tcgttcttga acggccacca gttcattcta cgtcctgttc tcatagccaa acctccacgg
     3241 atgaaaacgg tcaaaaaaca atgaccccct tgataaagcc cgggctcttt ccggcagcgg
     3301 tccggaaggc ctcattgatc tcgaacagag ggaagcggtg ggtgaccagg ggtttcatca
     3361 ccagcttttt cgcttccatc aattccagcc caatgttcat gccctcgatg tagagatggg
     3421 cgctgcggga atggccgttg acgatctgga aggccatcca gttccagtag ccccaatcca
     3481 cctgccgggg accaccctgg tgaaaaccga agacctcgag cttgccctcc atgcgcacca
     3541 gggcggcggt caggtcgagg gtctcctggc ggcccgccgc ttcgacgccg atatcgacgc
     3601 ccttgccgcc ggtaaggtcc cggacagcct gggccacatc ggcggtgcgc ggattgaagg
     3661 tgtaggcggc gccgagctgc tgcgccagct ggaggatctc ctcgcgcgta tcgacggcaa
     3721 tgatcatccc cgcgccgcgc gcacggaaga cctgcaacat gatcaagccc ataaagccgc
     3781 agccgaccag gcagacgctc tggttgaact gcacatccgc cttgcgcaca ccgttgacgg
     3841 cgcaggcgat tggctcgccc agggccagtt ccatcggggt ttcgggcctg aggcggtaga
     3901 gggagccgaa atgaaccgca aaataatcgg catagccctg gccttcgatc caggccgcca
     3961 catgatcgcc cgcctgatat tcccggacat tggcgccaac cttttcgatg atgcctgagg
     4021 cttcgtggcc gatgaagatc ggaaatttga tgccgggatt cttgccctcc cacatctcca
     4081 cctctcccgt gcagaccccg caggcggtgg ttttcaccag cacctggtcg ggcgtgatgg
     4141 agggaatcgg ccgttcctcg atgacgaact tgccggggcg gtgcagtatc gcagctttca
     4201 tggcggtcac ctggctatta tgaatgcttt gttcagtgtc gtatcccgag gacacccggc
     4261 cgcgagccgg tcggttctgc ggccggggag tccgctcggg agtatttcaa cttgtttcgg
     4321 aaaatccagc cattatttga gcagcatggc cttgcgcgac gagatcaact gatcattaac
     4381 ccgcagctgg aagaaataga tgccgctggc cagatcgccg gcgtcccatt tcaccgaata
     4441 ggtgccggcc gcctgcctgc gttcgaccag attgcccacc tttttcccca gcacattata
     4501 aacatcgatg cagacccggg catccttctg gagccggtag gtcagcgtgg tcgacgggtt
     4561 gaaggggttg ggatagttct gggtcagggt gaacatttcg ggcaggcttc cgcgctcgag
     4621 ctcatagtca ttgacgccgg tgatgctctg tttgaaggtc agcatgccga agcacttggc
     4681 gcggcgctgg atatccccgg cgatatagtc gctgggataa ccgacggcgg tgtcgccggg
     4741 ctggggatgc caggcgtagt aggcgtaggt gccttcgccc cattcccacg gtacggccgt
     4801 ggtatcggga gtcgctgccg ccccgccaat attaaagccg atgcgggtgc cgagagcgat
     4861 ctgcgggacg taaatcttca tctcgacgcc ccaggtaaga tcctcattgt tgacaaagac
     4921 ctggccgaca acccagctgg tatccaccgg cagcacctcc caccaggtct gattgggatc
     4981 atcccaccag ccgccccagt tcgaggtaat gcccttgtca ttgatcttgt agacggtcgg
     5041 gcccttgtcc ggggcgttcc agggggcgcc gctccagtca tcggcatagt taccgcccgc
     5101 gggatcgcca tacatcggtc catcgatgcc gaccatgatc tgttcaccga tccagttggc
     5161 agtatccggg ccgaaaaaga attcataatc ctcgaccttg gcgtagacgt agagccagtc
     5221 attcgaccag agcagtttgg cgttggcctc gatgc
//
LOCUS       DAOPWU010000243         5244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15793_length_5244_cov_4.583157, whole genome shotgun sequence.
ACCESSION   DAOPWU010000243 DAOPWU010000000
VERSION     DAOPWU010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15793_length_5244_cov_4.583157"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PKM23_11295"
     CDS             complement(<1..1272)
                     /locus_tag="PKM23_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNY92098.1"
                     /translation="MALIFMVGGAAAQSAGVQAEQASAPALTQGSGQAAQVVGGAAPG
                     MAPGSAPEAPGAVSPAPQGELALFGRHFFAAAQEPAGAGSAGDDYTLGAGDRLAIHLG
                     GKAQEHFETIVTADGKIYLPTAGVLSVQGMTMAQARQLLDLRLRRLYSGYTLDLVLLA
                     PKMVRVAVTGDVKQPGNYTLSALNSVLDAVSIAQGPTEHGSLRDIQVYRGENLAASID
                     LYSHLLKPGQSADVLLQSGDKIFVPPGRLRVEITGEVFRPAIYELAPGRPEDLGGLIA
                     LAGGFTGLAFRSKIEHSRMLPDGSRRISYYNLAADSAAGPELADGDRICVYSVKEQTP
                     RQTVTINGEVNRPGSYEWEAGMRVSDLILKAGSLTRSAWLLKAEVAEVDPNRPPRLRE
                     IPLERLMQGRGDSLDLVLGADDQLFIRRIPDW"
     assembly_gap    1478..1577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1781..>5244)
                     /locus_tag="PKM23_11300"
     CDS             complement(1781..>5244)
                     /locus_tag="PKM23_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY92099.1"
                     /translation="SPTELRVAFSEALEKKSAETPANYTISPAVNVTGALLQSDGKSV
                     LLTTAAHAAGAAYTLTVKGVKDLGIPRNSLAAGSTLTYTAPGGAADSDAEPPTITLAR
                     LTSATSLSVYFSEPLDEASAVALGNYFISNSVTVKSAALTAAGNVVQLTTSPHSAGQN
                     YILTVNNVCDRSARKNPLLPNSYYAYAYDPGDQIGPMITLVNAKDADQLEILFNEPLD
                     EASAETVANYTIQGDIQVLAAELDPSGQIVYLRTSLHSPDRLYLLNVRNVCDASAHKN
                     PVAGGTAFGYVFKPVDHIGPMISRVVVKDATHLQVIFSEELERTSAENRDNYRISDGV
                     VVMNAALDLEGKSVELETTPHLSGRVYILVINRVTDGSTVANEILPNSSYAYVHGNDD
                     LAAGPTIVRAAARSATQLVIEFSKPLEKNSAEKTANYQLNRGAAVTAALLNADNTSVT
                     LTTTPHEAGKVYILTVNDVSDATVYRRTVPANSAYSYLYEGSDTVGPVVSLVQVNSTT
                     SLDVMFNEQVEKSGAENLANYTISGNVTVLAARLDGSQRIVHLTTTAHEPQKLYVLRI
                     SAIRDASAEANPIAPNTSYSYLYEPADALPPTIAMVRVVDLQHLEVSFSEEVEAATAG
                     SLSSYALSSGKVLSVKAGGSGHQYILEVEPLTSGRILLLMVNGVRDGAGNTIAANSSY
                     AFSFGQIAAEPVPVVTGVTALSETQLQATFSVPLDKVSAERTANYQIQGVTVLSAKLD
                     AAKNKVWLTTSPHALNRIYVVLFSNIGRSDRPDLIVEANSPCFYMVSKSAAAAPGVLR
                     IVAAGEMLVEVWFNQPVERYSAEARRSYHISDDVAVLGAELDAGLNKVTLTTSRHRAA
                     QAYTLTVSGVSGAESGAVMSGTVLAYTFVPSLQVALAGSAEANLSFVAVGSPYYVDRN
                     YVITSAPEDLIHARMIMTANSDKGSSSTGFMQVTVSQATVVYVAYDASAQSVPAWLSS
                     RYTKTGLSLGVSESGEKLNLWAAYFTAGNITLGGNNAAGARGAKCMYVVLLQEPAFAR
                     TLSSGELEGLKEMSTQRPTTITLLPNYPNPFNPRTSIRFELPYDKEVRLVVYDILGRH
                     VRTLYESTASAGVHALIWDGVNDDQIPVAAGVYIYRLEVWENAQRNGVTYRNNYVTIT
                     RKMTYLK"
BASE COUNT          858 a         1485 c         1838 g          963 t
ORIGIN      
        1 ccagtcgggg atgcggcgga tgaaaagctg gtcgtcggcg ccgagcacga ggtcaagcga
       61 atccccgcgc ccctgcatca gccgttcgag cgggatctcc cggaggcgcg gcggccggtt
      121 gggatcgacc tcagccacct cggccttgag cagccaggcg ctgcgcgtga ggctgccggc
      181 cttgaggatg aggtcgctga cgcgcatgcc ggcctcccac tcatagctgc cggggcggtt
      241 cacctccccg ttgatggtga cagtctggcg cggggtctgc tccttgaccg aatagacgca
      301 aatccggtcc ccgtcggcga gttcgggtcc ggcagcggag tcagccgcga gattgtaata
      361 gctgatccgg cgcgagccgt ccggcagcat gcggctgtgt tcgatcttgc tgcgaaaggc
      421 gaggccggta aaaccgccgg cgagcgcgat caggccgccg aggtcctccg gtctgccggg
      481 cgccagttca tagatggcgg gacggaagac ttcaccggtg atctcgacgc gcagccggcc
      541 tggggggacg aagatcttgt cgccgctctg gaggagcaca tcggcggatt ggccgggctt
      601 gaggagatgg ctgtagaggt cgatgctggc ggccagattc tcgccgcggt agacctggat
      661 atcgcgcagg gagccgtgtt cggtcgggcc ctgggcgatc gagaccgcat cgaggacgct
      721 gttgagggcg gagagggtat agttgccggg ctgcttgaca tcgccggtca cggccacgcg
      781 caccatcttg ggggcgagca gcaccaggtc gagggtatag ccgctgtaga ggcggcgcag
      841 acggaggtcg agcagctggc gcgcctgggc catggtcatg ccctggacgc tgagcacgcc
      901 ggcggtggga agatagatct tgccgtcggc ggtgacgatg gtctcaaaat gctcctgggc
      961 cttgccgccg agatggatgg cgagacggtc gccggcgccg agggtgtaat catcccccgc
     1021 gctgccggct ccggccggct cctgggcggc ggcgaaaaaa tggcggccga agagggccag
     1081 ctccccctga ggcgcggggc tgactgctcc gggggcctcc ggagcgctgc cgggcgccat
     1141 gcccggggca gcaccgccaa cgacttgcgc ggcctggccc gatccctgcg tgagggccgg
     1201 ggcggaggct tgctcggcct ggacgccggc ggactgggcc gctgcgcctc ccaccataaa
     1261 gatcaaggcc atggccactg ctgctaccca acaggctgta tgcgttttca ccgggttgct
     1321 ccccattctt tcagtctgta tgaccacacc cacgccccgc gcagtggaca tcccgcgccg
     1381 atggtgtcaa atttttggac agaatatgta aaatatagtc aaaattagcc ctcaagtcaa
     1441 caataatcac tactttcaaa gtaacaccgc tgcggagnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnagc accctgtgat gaggatcgct ttttgaatga ataaccgggt
     1621 cggagcgagg ggggaatagt aacaccgctg cggagagcac cctgtgatga ggatcgcttt
     1681 ttgaatgaat aaccgggtcg gagcgagggg caaaaaaaaa gcgccgtgtg ctccctcccg
     1741 atgcacccgg cgcttccgtg ctggcaagct ggtcattgct ttatttcaga taggtcatct
     1801 ttcgcgtgat ggtgacgtag ttattgcgat aggtgacgcc gttgcgctgc gcattctccc
     1861 aaacttccag ccgatagata tagacccctg ccgcaaccgg gatctggtcg tcatttaccc
     1921 cgtcccagat cagggcgtgg accccggccg aggcggtgga ctcgtagagg gtgcggacat
     1981 ggcggccgag aatgtcgtag accaccaggc gcacctcctt gtcatagggc agctcgaaac
     2041 ggatcgaggt gcgcggattg aagggattgg gatagttggg cagcagggtg atggtggtcg
     2101 gccgctgcgt gctcatctcc ttcaatccct ccagttcgcc gctgctgaga gtgcgggcaa
     2161 aggccggctc ctgcagcagc accacataca tacatttggc cccgcgcgcg ccggcggcgt
     2221 tgttgccgcc cagggtgatg ttgccggcgg tgaaataggc tgcccagagg ttgagttttt
     2281 cgccgctctc ggagacaccc aggctgagcc cggttttggt atagcgcgag gagagccagg
     2341 ccggcaccga ctgggcgctg gcatcatagg cgacatagac caccgtggcc tggctcaccg
     2401 tcacctgcat gaacccggtg gagctgctgc ccttgtcgct gttggcggtc atgatcatcc
     2461 tggcgtggat gagatcctcg ggggccgagg tgatgacata gttgcggtcg acgtagtatg
     2521 gggagcccac cgcgacgaag gagagattcg cctccgcact gccggccagc gcgacctgca
     2581 gcgaagggac gaaggtgtag gccagcaccg tcccgctcat caccgcgccg ctctccgcac
     2641 cgctgacccc ggagacggtc agggtgtagg cctgcgcggc gcgatggcgc gaggtggtga
     2701 gggtgacctt gttgaggccg gcatcgagtt cggccccgag caccgccaca tcatcggaga
     2761 tatgatagct gcggcgggct tcggcgctgt agcgctccac cggctgattg aaccagacct
     2821 cgaccagcat ctcgccggcc gcgacaatgc gcagcactcc gggggcggca gccgcggatt
     2881 tcgacaccat gtagaaacag gggctgttgg cctcgacgat caggtcgggc cggtcgctgc
     2941 ggccgatgtt gctgaacaag accacataga tgcggttgag ggcatggggg gaggtggtca
     3001 gccagacctt gtttttggcg gcgtcgagct tggccgagag caccgtcacc ccctggatct
     3061 gatagttggc cgtcctttcc gccgagacct tgtcgagcgg cacgctgaag gtggcctgga
     3121 gctgggtctc gctcagggcc gtcactccgg tcaccaccgg cacaggctcg gcggcgatct
     3181 ggccgaagct gaaggcatag gagctgttgg cggcgatggt gttgcccgcc ccgtcgcgca
     3241 ccccgttgac catgagcagc aggatgcgtc cgctcgtcag gggttcgacc tcgaggatgt
     3301 actgatggcc tgaaccgccc gccttgacgc tgagaacctt gccgctgctc agggcgtagc
     3361 tggaaaggct gcccgccgtg gccgcctcga cctcctcact gaagctgacc tcgagatgct
     3421 ggaggtcgac cacccgcacc atggcgatgg tgggcgggag ggcgtccgcc ggctcgtaga
     3481 gataggagta gctggtgttg ggggcgatcg gattggcctc cgcgctggca tcccggatcg
     3541 cgctgatgcg cagcacatag agcttttgcg gctcatgggc ggtggtggtg agatggacga
     3601 tacgctggga gccgtccagc cgggctgcga gcaccgtgac gttgccgctg atcgtatagt
     3661 tggcgaggtt ctccgctccg ctcttctcca cctgctcgtt gaacatgaca tcgaggctgg
     3721 tggtgctgtt gacctgaacc agcgagacca ccgggccgac cgtgtcgctg ccctcgtaga
     3781 ggtagctgta ggcgctgttc gccggcacgg tgcgccggta gacggtggcg tcgctgacat
     3841 cattgaccgt aaggatatag accttgcccg cctcatgggg agtggtagtc agggtcacag
     3901 aagtgttgtc cgcattgaga agggcggcgg tgacggccgc gccgcggttg agctggtaat
     3961 tggccgtctt ttcggcgctg ttcttttcga ggggcttgct gaactcgatc accagctggg
     4021 tagcgctgcg ggcggcggcg cgcacaatgg tcgggccggc ggcgagatca tcgttgccgt
     4081 gaacgtaggc ataggagctg ttgggcagga tctcgttggc gaccgtcgaa ccatccgtca
     4141 cccggttgat gaccaagatg tagacgcggc cggagagatg gggggtcgtc tccagctcca
     4201 cgcttttgcc ctccaggtcg agggctgcgt tcatcaccac cacgccgtcg ctgatgcgat
     4261 agttgtcgcg gttctccgcg ctcgtgcgct ccagctcctc gctgaaaatg acctggagat
     4321 gggtggcgtc cttgaccacc acccggctga tcatcgggcc gatgtggtcg accggcttga
     4381 agacgtagcc gaaggcggtg ccgccggcga ccggattttt gtgcgcgctg gcgtcgcaga
     4441 cattgcggac attgagcagg tagaggcggt cggggctgtg caggctcgtg cgcagataga
     4501 cgatctggcc ggaaggatcg agttcggcgg cgaggacctg gatgtcgccc tggatcgtat
     4561 agttggcgac ggtctcggcg ctggcctcgt cgagcggctc attgaagagg atctcgagct
     4621 ggtccgcatc tttggcattg accagggtga tcatcgggcc gatctggtcg cccgggtcat
     4681 aggcataggc gtaatagctg ttggggagga ggggattttt gcgcgccgaa cggtcgcaga
     4741 cgttgttgac ggtgaggata tagttctggc cggcgctgtg ggggctggtg gtgagctgga
     4801 cgacattgcc cgccgcggtg agggcggccg acttgacggt gaccgaattg ctgatgaaat
     4861 agttgccgag agcgacggcc gaggcctcat cgagaggctc gctgaaatag accgagaggc
     4921 tggtcgccga ggtgagccgc gccagggtga tggtgggcgg ctcggcatcg ctgtcggcgg
     4981 ccccgccggg ggcggtgtag gtgagggtgc tgccggcggc caggctgttg cgggggatgc
     5041 ccagatcctt gacccccttg acggtgaggg tgtaggccgc gccggcggca tgggcggcgg
     5101 tggtcaggag cacgctcttg ccgtcgctct ggaggagggc gccggtgaca ttcaccgccg
     5161 gggagatggt atagttggcg ggggtctcgg cgctcttttt ctcgagggct tcgctaaagg
     5221 cgacgcgcag ctcggtgggg ctga
//
LOCUS       DAOPWU010000244         5232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15843_length_5232_cov_4.855128, whole genome shotgun sequence.
ACCESSION   DAOPWU010000244 DAOPWU010000000
VERSION     DAOPWU010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15843_length_5232_cov_4.855128"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2227)
                     /locus_tag="PKM23_11305"
     CDS             complement(<1..2227)
                     /locus_tag="PKM23_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92100.1"
                     /translation="MKVRLLMMLTLSTLLAQAGAQTTHGSYQNRARNIHAGNLIRVTF
                     YNHGMVGGIKGDNSLIYAGEWPINSGHIQMGNASMYVSSALRTFAGIDSVGDSTYTVI
                     TPCVFCQGWDPNIFSHDSLGVFLGFEPLPGYYDLANKEADPYHAIAMSHQAYTWPSIW
                     PDKMDDATKPGWAGSWNGYFGRDQKNSDEESFYAMDDWSFKKKVDGLPLPRPIASESQ
                     RGGIGLRQNVRGLQWSNPDAQDCIFWIYEIKNIGELYHTKTIFGLNVGASIGATISDN
                     TDYDDDCATFYRELDLTVNYDWDNIGTAGYSPVPWVGYAFLESPGNARDGIDNDGDGI
                     NGGGHLLTTEDFLRSYMVGDPVVLIDYSSDEYTRTVGSMPAEGVTIRQNGTTYIKKPG
                     APLMEIERNGIDDNLNGFIDESDGAVAPDSMAYYLYIRDAKYNNQDYLAKDYLTGEGL
                     TNLLIDERRDDGIDNDGDWDIKTDDMGMDGKSGSGDPGEGDGLPTAGIGDLPGEPNVD
                     HTDVDESDQIGLTSFVFYEYGNITYSNDAQMWDESAPGYFDGHLENVDADYIFSCGYF
                     PLNPGQQEFFSVAMVYGDDQQDILRNKDIVQKIYNSNYNFAVAPEKPKLRAVAGDRKV
                     TLFWDSRAEESVDRYLHEYDFEGYKIYRATDPGFKDAGSISDGYGYTRYVKPLAIYDK
                     VDSVFGFYQNTFGTGVQFNLGNETGLVHVFVDSPLVNGRRYFYAVNAYDRGSIEKNIA
                     PS"
     gene            complement(2251..3324)
                     /locus_tag="PKM23_11310"
     CDS             complement(2251..3324)
                     /locus_tag="PKM23_11310"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY92101.1"
                     /translation="MNKRVKLLQQRTSAGNLLLAAAVLLFLLAGTAPLLAQKVSGVGT
                     SSAAFLKIGVGARALGMGEAVATQAEDATAMFWNPAGLAKIERMQILLNHYDYLADMS
                     FEFAGIAIPVRNVGSFGLFFSYLGMPDIERTTITYPDGNGEMVSASSFAAGLSYARSL
                     TDRFAIGGSAKLIHESIWHSDASGLAMDVGIQYRTFFKNLKIGMSISNFGSDMQIEGR
                     DMLVQHDIDQTSDGNNGSINAQLSTDQFPLPILFRVGLSANLAEDFFGIQEHDFIVAV
                     DAIHPSDNKEYLNVGAEYTFKQLLSLRGGYRQLFLADTQGGLTLGMGLHARVLRTEVM
                     FDYAMIDYGLLDRQNKFSLILAL"
     gene            complement(3345..>5232)
                     /locus_tag="PKM23_11315"
     CDS             complement(3345..>5232)
                     /locus_tag="PKM23_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY92102.1"
                     /translation="GFQSPVGDTVHTFAAADNQMVPLGWYKGYSSQAKFFLDASSKLK
                     LSYGLYYNYDRSYGVGWNYKYVPDAGKFYNTRSQTHIFSAKYIASKSTFIDFKANYYD
                     KAHKNFLYEDPYDPRYMPTESAMIEQYVYGRRAEDNISVISRVNDFTYYGNSTDIGKN
                     DVEYYNFSLDLTSQINKRHLVKIGAATTLNDLRNDWFQLQFSDVTFRPIVPDESSPFH
                     VKYGVKPKEYSAYIQDKIEFNELIINIGLRYDRFDPDGRVLADPMDPQIYSPFKMDHI
                     YRNYTPSTPDSELVKYTVAEREGFWWKKAQAKSQLSPRFGLSFPITDKGVIHFSYGWF
                     FQNPQLSYLYDNPNFWITGAGASNLVGNADLNAERTVMYELGLQQQLSENLYLHLTGF
                     YRDIRDWLGTGEPIDTYQGMTYYMYVNKDHAAAKGVTLSASYRLSKLAINLDYTYMTA
                     KGTSSDPQDAYYDALAKEAPRVQMIDLNWDQRQALNTVISYANRGWSTSLVSTINSGL
                     PYTPSFARGEVSGSGTFTGLRENSERKPLTYNLDLRVGRLVNIGTFRAQIFCNITNLL
                     DTRNATGIYSDTGQPDYTLQGINQVDRVGMPDVEISNVAEYFTRPGNYSSPRFIQFGL
                     RLSK"
BASE COUNT         1064 a         1358 c         1677 g         1133 t
ORIGIN      
        1 ccgagggggc gatgttcttt tcgatgctgc cccggtcgta ggcgttcacg gcatagaaat
       61 agcgccggcc gttgacgagc ggggaatcga caaagacatg caccagcccg gtctcgttgc
      121 cgaggttgaa ctggacgccg gtgccgaagg tgttctgata aaagccgaag acgctgtcga
      181 ccttgtcgta aatggccagg ggcttgacgt agcgggtgta gccgtagcca tcgctgatcg
      241 agccggcatc cttgaagccc ggatcggtgg cgcggtagat cttgtagccc tcaaagtcgt
      301 actcatgcag gtagcggtcc acgctctcct ccgcacggct gtcccagaag agggtgacct
      361 tgcggtcgcc ggcgacggcg cgcagtttgg gtttttccgg ggcgacggcg aagttatagt
      421 tggaattata gatcttttgc acgatatcct tgttgcgcag aatatcctgc tgatcatcac
      481 cataaaccat ggcgaccgag aagaactctt gctggccggg attgaggggg aagtagccgc
      541 aggagaagat atagtcggca tcgacgttct cgagatgacc gtcgaaatag ccgggggccg
      601 actcgtccca catctgggcg tcattgctgt aggtgatgtt gccgtattca taaaagacaa
      661 agctggtgag gccgatctgg tccgattcgt cgacgtcggt gtggtcgacg ttgggctcgc
      721 cgggcagatc gccgatgccg gctgtgggca ggccgtcccc ttcgccgggg tcgccggatc
      781 cgctcttgcc gtccatgccc atgtcatcgg tcttgatatc ccagtctccg tcgttgtcga
      841 tgccgtcatc gcggcgttcg tcgatgagca ggttggtcag cccctcgccg gtgagataat
      901 ccttggcgag atagtcctgg ttattgtact tggcgtcgcg gatgtagagg taataggcca
      961 ttgaatcggg ggccacagcg ccgtcggact cgtcgatgaa gccgttgaga ttatcgtcga
     1021 tgccattgcg ctcgatctcc atcaggggcg cgcccggctt tttgatatag gtcgtgccgt
     1081 tttggcgaat ggtcaccccc tcggccggca tggagccgac agtgcgggta tattcgtcac
     1141 tgctatagtc gatgaggacg accggatccc cgaccatata gctgcgcaga aagtcctcgg
     1201 tggtgaggag gtggccgccg ccgttgatgc cgtctccatc attgtcgatg ccgtccctgg
     1261 cattgcccgg gctttcgaga aaggcgtagc ccacccaggg cacagggctg taaccggcag
     1321 tgccgatatt atcccagtca tagttgacgg tcagatcgag ttcgcgataa aaggtggcgc
     1381 agtcgtcgtc ataatcggtg ttgtcgctga tggtcgcgcc gatggaggcg ccgacgttga
     1441 ggccgaagat ggtcttggtg tggtagagtt caccgatatt cttgatctca tagatccaga
     1501 agatgcagtc ctgggcatcg ggattggacc actgcaggcc gcggacgttc tggcgcaggc
     1561 cgatgccgcc gcgctgtgac tccgaggcga tcggacgcgg caggggcagg ccgtcgacct
     1621 tcttcttgaa gctccagtca tccatggcat aaaaggactc ttcatcggag ttcttctggt
     1681 cgcggccgaa atagccgttc cagctgccgg cccagccggg tttggtggcg tcatccatct
     1741 tgtccggcca tatcgagggc caggtgtagg cctggtggct catggcgatg gcgtgatagg
     1801 gatcggcctc cttgttggcc aggtcataat agccgggcag gggctcgaag ccgagaaaga
     1861 cgcccagaga atcgtgcgag aaaatattgg ggtcccaccc ctggcagaag acgcaggggg
     1921 tgatcaccgt ataggtggag tcgccgaccg aatcgatgcc ggcgaaggtg cgcagcgcgg
     1981 agctgacgta catggaggcg ttgcccatct ggatatgacc ggagttaatg ggccattcgc
     2041 cggcatagat caggctgttg tcgcccttga tcccgccgac catgccgtgg ttgtaaaagg
     2101 tgacgcgaat caggttgccc gcgtgaatgt tgcgggcacg gttctgataa ctgccgtgcg
     2161 tggtctgcgc tcccgcctgg gccagaagtg tggagagcgt cagcatcatc aaaagacgta
     2221 ctttcatgga aattctcctt ccggctctgc ttacagcgct aaaatgagcg agaacttgtt
     2281 ttgcctgtcg aggaggccat aatcgatcat ggcataatcg aacataactt cggtgcggag
     2341 caccctggcg tgcagcccca tgccgagggt gaggccgccc tgggtgtcgg cgagaaaaag
     2401 ctgccggtag ccgccgcgca gcgacaagag ctgcttgaag gtatactcgg cgccgacatt
     2461 gagatactcc ttgttgtcac tgggatggat ggcatccacg gcgacgatga aatcatgctc
     2521 ctgaatcccg aagaaatcct cggccagatt ggcggagagg ccgacccgga agaggatcgg
     2581 cagggggaac tgatcggtcg agagctgggc gttgatgctg ccattgttgc catcgctggt
     2641 ctggtcgatg tcgtgctgga ccagcatgtc gcggccttcg atctgcatgt ccgaaccgaa
     2701 attggagatc gacatgccga ttttgaggtt cttgaaaaag gtgcggtact ggatgccgac
     2761 atccatggcc agcccgctgg catcgctgtg ccagattgat tcgtggatga gcttggccga
     2821 gccgccgatg gcaaaccggt cggtcaggct gcgggcatag ctgagtccgg cggcgaaaga
     2881 gctggcggag accatttcgc cgttgccgtc cggataggtg atggtggtgc gctcgatatc
     2941 gggcatgccg agataggaga aaaatagacc gaaggaaccc acattgcgca cggggatggc
     3001 gatgccggcg aattcgaagg acatatccgc cagatagtcg tagtggttga gcaggatttg
     3061 catgcgctcg atcttggcca gtccggcggg attccagaac atcgcagtag catcttcggc
     3121 ctgggtggcg acggcttcgc ccatgcccag ggcgcgggca ccgaccccga ttttcagaaa
     3181 ggcggccgag ctggtgccca ctccgctcac cttctgggcg aggagcgggg ccgtcccggc
     3241 gagcaggaac agcagtacag cagcagccag caacagattc ccggcgcttg ttctctgctg
     3301 cagaagtttt actcttttat tcatcatgac catcctcttt ttgcttactt gctcagtcgc
     3361 aacccgaatt gaataaaacg cggcgaggag taattgccgg gcctggtgaa atactcagcg
     3421 acgttggaga tctcgacatc cggcatcccc acgcggtcga cctgattgat cccctgcaag
     3481 gtgtaatcgg gctggccggt gtcagaatag atgccggtgg cgttgcgggt gtcgagcaga
     3541 ttggtgatgt tgcaaaaaat ctgggcccgg aaggtgccga tattgaccaa gcggccgacg
     3601 cgaagatcga gattataggt gaggggcttg cgctcgctgt tctcgcgcag gcccgtaaag
     3661 gtgccggaac ccgaaacctc accgcgcgca aaagaggggg tgtagggcag gcccgaatta
     3721 atcgtgctga ccagactggt cgaccagccg cggttggcgt agctgatcac cgtattgagg
     3781 gcctgacgct gatcccagtt gaggtcgatc atctggacgc gcggtgcctc cttggcgagg
     3841 gcgtcataat aagcgtcctg tggatccgaa gaggtcccct tggcggtcat gtaggtataa
     3901 tcgagattga tcgccagctt gctgagacgg tagctggccg aaagcgtgac ccccttggcc
     3961 gccgcatgat ccttgttgac atacatgtaa taggtcatgc cctgataggt gtcaatgggc
     4021 tccccggtcc ccagccaatc gcggatatca cgataaaagc cggtgagatg gagatagaga
     4081 ttctccgaga gctgctgctg gagtccgagt tcgtacatca ccgtgcgctc ggcattgaga
     4141 tcggcattgc cgaccagatt gctggcgccg gctccggtga tccaaaaatt gggattgtcg
     4201 tagagatagc tgagctgggg attctggaaa aaccagccgt agctgaaatg gatgaccccc
     4261 ttgtcggtga tcggaaaact gagcccgaag cgcggcgaga gctgcgactt ggcctgggcc
     4321 tttttccacc aaaagccctc ccgttcggcg acagtatact tgaccagctc gctgtcgggc
     4381 gtggaggggg tatagttccg gtaaatatgg tccatcttga agggggagta gatctgggga
     4441 tccatcggat cggccaggac gcgcccgtcc ggatcgaagc ggtcatagcg caaaccgata
     4501 ttgatgatca attcattgaa ctcgatcttg tcctggatgt aggccgaata ctccttgggc
     4561 ttgacgccat acttgacatg gaatggcgag ctctcatccg ggacgatcgg acggaaggtg
     4621 acgtcggaga actggagctg gaaccagtcg ttgcgcagat cattcaaggt ggtcgcggca
     4681 ccaatcttga cgagatggcg cttgttgatc tggctggtca gatcgaggct gaaattgtaa
     4741 tattcgacat cgttcttgcc gatatcggtc gaattgccat aataggtgaa atcattgacg
     4801 cgggagatga ccgagatgtt gtcctccgcc ctgcggccgt aaacatactg ctcaatcatc
     4861 gccgattcag tcggcatgta gcgaggatcg tacgggtcct catagagaaa attcttgtgg
     4921 gccttgtcgt aatagttggc cttgaaatcg ataaaggtgc tcttggaggc gatatacttg
     4981 gccgaaaaga tatgggtctg actgcgggta ttgtagaact tgccggcgtc cggcacatat
     5041 ttgtagttcc agccgacccc gtagctgcgg tcatagttgt agtagaggcc gtaggagagc
     5101 ttgagcttgc tcgaagcatc gaggaagaat ttggcctggg aggaatagcc cttgtaccag
     5161 cccaggggca ccatctggtt gtcggccgcg gcgaaggtgt ggacggtatc gccgaccggg
     5221 gactggaagc cc
//
LOCUS       DAOPWU010000245         5223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15879_length_5223_cov_4.426858, whole genome shotgun sequence.
ACCESSION   DAOPWU010000245 DAOPWU010000000
VERSION     DAOPWU010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15879_length_5223_cov_4.426858"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PKM23_11320"
     CDS             complement(<1..835)
                     /locus_tag="PKM23_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNY92103.1"
                     /translation="MITFRDPSTRKMLLLLTALVLIVLLCAIGGIAQMLIIALLLAYI
                     LDPLVIRMEARGISRTAAATIILLTMTALIVIGIITVVPALKAQMVGMSSGESSAKTD
                     LALANLQEVVRTKMGWLGFGDIDLKVKLAEFKASFGKRIVDFIVSDFVGIVLALVTIP
                     FLMFFLIKDALTLKKGLIRIVPNRYFEFSLDLIHKMDQQLGNYLRGQFIDAVSIGTLA
                     TIGLGALGVPYFFLLGPFSGLANLIPYVGPFAGGIPSMIIAVMENGDLSAGGNVALLF
                     LG"
     gene            complement(832..1368)
                     /locus_tag="PKM23_11325"
     CDS             complement(832..1368)
                     /locus_tag="PKM23_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNY92104.1"
                     /translation="MKGLFVKKKAAHPEQDDAELLKVLKALPVFENLDRRELAAVLRI
                     LHEREYQPDEVVFREEEPGMGMYIIQKGRVMIRSEKGNLLLAELGDGAFFGEISLLDA
                     SPRSATVIAKEKSRIFGFFQPDLYGLIERNPTAGVKIVLGLSRLVCERLRQTNKRAYD
                     FNEALQQMRQAPKQEKPA"
     gene            complement(1499..2977)
                     /locus_tag="PKM23_11330"
     CDS             complement(1499..2977)
                     /locus_tag="PKM23_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNY92105.1"
                     /translation="MANRDFADLLQSQIVEDLGRLYDPAWNRRMLMDADYSEAMRPQV
                     PCALLELLSHQNFLDMKFALDPRFRFDTARAIYKAMLRFIAFQNRSSYVVQPLPVDHF
                     QALLSAPGEAILQWQPVADALEPTAAPEGYIVYTRREEGGFDNGVRVDEPRLVVQGIE
                     PGVLYSFKVTACNAGGESFPSETLALCRMADDAPPVLVVNAFDRLSAPAWFEGGDLSG
                     FADFIDAGVADRLSIGYTGRQTDLVTASPFRTNDAPGHGASQAAWEARPIAGNSFDYP
                     ALHGRSIRAAGRSFCASSDEALAAGQVDIRAFRIVDLIFGEEKRTSWPTPAGDSLRGP
                     RYEAFTPGLCAAIDTLLSRRGGLLLSGAYIGSDPCAGRTPEDPIVQFIEKKLHYRWTA
                     GHASSDGRLFSTDERFWPKGAQFGFNTLPDEHIYAVEAPDAIDPVNGGRTILRYAENQ
                     FSAAVTYKKEYAVAALGFPLEAVINAGERDRLMAALLTFLTK"
     gene            complement(2910..4517)
                     /locus_tag="PKM23_11335"
     CDS             complement(2910..4517)
                     /locus_tag="PKM23_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92106.1"
                     /translation="MKSRTLLTAAALGAALVLACAPRVAVRKPAYDYSKMDKESARIH
                     KKVEALIDECFTGREPLPLGPGVRILSLLHEPERKTLAVTFNRNFSYPPFRPGNVRAT
                     YARLRELLGGKYREYSITLWSQGVPIEALIPNAFRNGSGQWDLRRMAAQNPRPEAVIR
                     RISRPLQPSQGLLNRTIDVRPSHGWYYNSTIDRWEWQRPRLFQTVEDLLPFSFTTFYL
                     IPMLENAGAVVFTPRERDIQTREVVVDNDAPGSGYRESGQGWRSGEGAGFAPGHPPYQ
                     VNHNPFLQGSHRLCAADSAGGSLAQWVPEIPADGDYAVYISWRHGPEQVSDALYTVHH
                     AGGATRFLVNQQIGGPTWIYLGTFRFLQGSRPEGGSVELSSRSATPGRLISADAVRFG
                     GGMGNILRNGSVSGKPRFAEGSKYYLQYAGLPDTLIYNLRRQGRLPRRLQQPQRVSQL
                     SLRRPLRAEEKPAGARARHPLRSHPCISHRCGHHPQRHHHRHPEHLQLSLRGFDLQLP
                     RRGLAHGQPRFRRSPPEPDCRGSGPAL"
     gene            4690..>5223
                     /locus_tag="PKM23_11340"
     CDS             4690..>5223
                     /locus_tag="PKM23_11340"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNY92107.1"
                     /translation="MHLQKMEENFLELIRRSSAFLPRDVEFILADARGREEPDSRARL
                     ALDMVALDIGLARQRSAPVCQDTGTITFYIRTPVGVDQMQIRLAIEAAVAEATARGYL
                     RQNSVDSLTGRNSGNNLGPGTPVCHFEQWAGEEIDVRLILKGGGCENTSAQFMLPATF
                     DGVRYNRDLEGAFACIMQ"
BASE COUNT          965 a         1531 c         1787 g          940 t
ORIGIN      
        1 ggccgaggaa gaggagggcg acgttgcccc ccgccgacag gtcgccgttc tccatcactg
       61 cgatgatcat cgagggaatc ccgccagcga aggggccgac gtagggaatg aggttggcca
      121 gccccgagaa ggggccgagc aggaaaaagt aggggacgcc caatgcgccg agaccgatgg
      181 tcgccagggt gccgatcgag accgcatcga tgaactgccc gcgcaaataa ttgcccagct
      241 gctggtccat tttatggatg agatcgaggc tgaactcgaa atagcggttg ggcacaatgc
      301 ggatcaatcc ctttttcagg gtcagggcat ccttgatgag aaagaacatc aggaagggga
      361 tggtgaccag ggcgaggacg atgccgacga aatcactgac gataaagtcg acgatccttt
      421 tgccgaaaga ggccttgaac tcggccagct tgaccttgag gtcgatatcc ccgaaaccga
      481 gccagcccat cttggtgcgc acaacctcct ggagattggc aagggcgagg tcggttttgg
      541 cggagctttc gccgctgctc attcccacca tctgtgcctt gagggccggg accacggtga
      601 tgatgccgat gacgatgagg gcggtcatgg tcaacaggat aatggtggcc gcggctgtgc
      661 gcgagatccc gcgcgcctcc atacggatga ccagggggtc gaggatatag gcgaggagga
      721 gggcgatgat cagcatctgg gcgatgccgc cgatggcgca gaggagaacg atcagcacca
      781 gggcggtgag caggagcagc atcttgcggg tgctgggatc gcgaaaggtg atcatgccgg
      841 cttctcctgc ttgggggcct gccgcatctg ctggagggct tcgttgaaat cataggcgcg
      901 tttgttggtc tggcgcagcc gctcgcagac cagacgggag aggccgagga cgatcttgac
      961 tccggcggtg ggattgcgct cgatcaggcc gtagagatcg ggctggaaaa agccgaagat
     1021 gcggctcttc tccttggcga tcacggtcgc ggagcgcggc gaggcgtcca gcagggagat
     1081 ctcgccgaaa aaggccccgt cgccgagctc ggccagcagc aaattgccct tttcggagcg
     1141 gatcatcacc cgccctttct ggatgatgta catccccatg cccggctcct cctcgcggaa
     1201 gacgacctcg tcgggctgat actctctttc atggagaatc ctcaacaccg cagccagctc
     1261 gcggcggtcg aggttttcaa agaccggcaa ggccttgagc accttgagca gttcagcatc
     1321 gtcctgttcg gggtgtgctg cttttttttt gacgaacaag cctttcatgg gctttccatc
     1381 ctcgcgcagt tagatggtcc ggaatctggt tcagggtcga caagcggggc tgtgtgggct
     1441 ccccgttccc ctcctggtag aatataatgc aaaatgacta aaaaatgaaa tctttttgtt
     1501 attttgtcaa aaatgtcagc agtgcggcca taagccggtc gcgctctccg gcattgatga
     1561 cggcctcgag cggaaagccc agtgcggcca cggcatactc ttttttgtag gtgacggcgg
     1621 cgctgaactg gttctccgca tagcggagga tggtgcggcc gccgttgacc ggatcgatcg
     1681 catccggggc ctcgaccgca tagatgtgct cgtccggcag ggtattgaag ccaaattgag
     1741 cgcccttggg ccagaagcgc tcgtcggtgc tgaagaggcg tccgtcgctg ctggcgtggc
     1801 cggctgtcca gcggtaatgg agtttcttct cgataaactg cacgatcgga tcctccggcg
     1861 tccggccggc gcaggggtcg ctgccgatgt aggctccgga gagcaggagc ccgcccctgc
     1921 ggctgagcag ggtgtcaatc gccgcgcaca gtcccggagt gaaagcctcg taacggggcc
     1981 cgcgcaggga gtcgccggca ggggtcggcc aggaggtgcg cttctcctcg ccgaagatca
     2041 agtcgacaat cctgaaggcg cggatatcta cttgcccggc ggccagagcc tcgtcactgc
     2101 tggcacagaa tgagcggccg gcggcgcgga tggagcggcc atgcagggcg gggtaatcga
     2161 agctgttgcc ggcgatcggc cgcgcctccc aggcggcctg gctggcgcca tggccggggg
     2221 catcattggt gcggaagggc gacgccgtca ccaggtcggt ctgccgcccg gtgtagccga
     2281 tgctgaggcg gtcggccacc ccggcgtcga tgaaatcggc gaatccggag aggtcgccgc
     2341 cctcaaacca ggccggggcc gagaggcggt cgaaggcgtt gaccaccagc acggggggag
     2401 cgtcatcggc catgcggcag agggccaggg tctcgctcgg aaaactctcc ccgcccgcat
     2461 tgcaggcggt caccttgaag ctgtagagca cgccgggctc gatcccctgc accaccagcc
     2521 ggggctcatc gacgcggacg ccgttgtcaa atccgccctc ctcgcggcgg gtgtagacga
     2581 tatagccctc cggcgcggcc gtgggctcga gggcatcagc gaccggctgc cattgcagga
     2641 tggcctcgcc cggggcgctg agcaaggcct ggaagtggtc gaccggcagg ggctggacga
     2701 catagctgct ccggttctga aaggcgatga atctgagcat cgccttgtag atcgcccgcg
     2761 cggtgtcgaa gcggaagcgg ggatcgaggg cgaacttcat gtcgagaaaa ttctgatggg
     2821 agagcagttc gagcagggcg cagggcacct gggggcgcat cgcctcgctg taatcggcgt
     2881 ccatgagcat gcgccggttc caggcgggat catagagccg gcccagatcc tcgacaatct
     2941 ggctctggag gagatcggcg aaatcgcggt tggccatgcg cgagaccccg tcggggaagc
     3001 tgaaggtcga atccgcggag ggataactgt agatgctcag ggtgccgatg gtggtgtcgc
     3061 tgcgggtgat gcccgcatcg gtgtgaaatg caagggtgag atcgaagggg atgccgagcc
     3121 cgggctccag ccggtttttc ttcggcccga agggggcgcc gtagagatag ttgagatact
     3181 cgctgcggct gttgtaatcg tcgcggtaat cgtccctgcc gccgtaggtt gtagatcagg
     3241 gtgtcgggca gccccgcata ctggagataa tatttgctcc cctcggcgaa gcgcggcttg
     3301 ccgctgactg acccgttgcg caaaatgttg cccatgcccc cgccgaaacg caccgcatcg
     3361 gcgctgatca gccggccagg ggtcgcgctc cggctggaga gctcgacgct gccgccctcg
     3421 ggccggcttc cctgcaggaa gcggaaggtg ccgaggtaga tccaggtcgg accgccgatc
     3481 tgctgattga cgagaaaacg ggtcgcaccc cccgcatgat ggacggtgta gagggcgtcg
     3541 ctgacctgct ccgggccatg gcgccaggag atgtagaccg catagtcgcc atcggccggg
     3601 atctcgggta cccactgcgc gaggctgcct cccgcactgt cggcggcgca gaggcggtgg
     3661 ctgccctgga ggaagggatt gtggttgacc tggtaggggg gatggccggg tgcgaacccc
     3721 gcaccctcgc cgctgcgcca gccctggccg ctctcgcgat agcccgaccc gggcgcgtcg
     3781 ttgtcgacca ccacctcacg ggtctggatg tcgcgttcgc gcggggtaaa gaccaccgcc
     3841 ccggcgttct caagcatggg gatgagataa aaggtggtga aggagaaggg gagcagatcc
     3901 tccacggtct ggaagaggcg cggacgctgc cattcccagc ggtcgatcgt gctgttgtag
     3961 taccagccat ggctggggcg cacatcgatg gtgcggttga gcagcccctg cgagggctgg
     4021 agagggcggc tgatgcggcg gatcaccgcc tcggggcgcg gattctgcgc ggccatgcgc
     4081 ctgaggtccc actgcccgct gccattccga aaagcgttgg gaatgagggc ctcgatcggc
     4141 acgccctggc tccagagggt gatgctgtac tcccggtatt tgccgccgag cagctcgcgg
     4201 aggcgggcgt aggtcgccct gacgttcccg ggccggaagg ggggatagga gaagttgcgg
     4261 ttgaaggtga ccgcgagggt tttccgctcg ggttcgtgga gcagggagag gatgcgcacg
     4321 ccgggaccaa ggggaagggg ctccctgccg gtgaagcatt catcgatcag cgcctcgacc
     4381 tttttgtgga tgcgggcgct ctccttgtcc atcttgctgt aatcgtaggc cggcttgcgg
     4441 accgccaccc ggggggcgca ggcgagcacc agggcggcgc ccagagcggc agcagtgagg
     4501 agggttctgc tcttcatcca tgctcctgat ttttaatgct ctaatgatac agaaaattgt
     4561 cctctcattc aagggaaaag tgcggctggt ggtgcgcctt ttacagctgc gggggagcgc
     4621 tgaataatcc cgctgtaaaa atggttaaga aaggtaaaag tcctgttcaa cgccgtccct
     4681 ggaggctcta tgcatctgca aaaaatggaa gaaaactttc tcgaactgat ccggcggagt
     4741 tcggcctttt tgccgcgcga tgtcgagttc atcctggccg atgcgcgcgg tcgcgaggag
     4801 cccgactccc gcgcccgcct cgcccttgac atggtcgccc tggatatcgg cctcgcccgc
     4861 cagcgctcgg cccccgtctg ccaggacacc ggcacgatca ccttttacat ccgcaccccg
     4921 gtcggcgtcg accagatgca gatccgcctc gccatcgagg ccgcggttgc agaggcaacc
     4981 gcccggggat acctgcgcca gaattcagtc gacagcctca ccggccgcaa cagcggcaac
     5041 aacctcggtc cgggcacacc ggtctgccac tttgaacagt gggccggaga ggagatcgat
     5101 gtccgcctta tcctcaaggg cggcggctgc gaaaacacca gcgcccagtt catgctcccc
     5161 gccaccttcg acggggtgcg ctataaccgg gacctcgagg gggcctttgc ctgcatcatg
     5221 cag
//
LOCUS       DAOPWU010000246         5220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15892_length_5220_cov_4.837948, whole genome shotgun sequence.
ACCESSION   DAOPWU010000246 DAOPWU010000000
VERSION     DAOPWU010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15892_length_5220_cov_4.837948"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PKM23_11345"
     CDS             complement(<1..1273)
                     /locus_tag="PKM23_11345"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNY92108.1"
                     /translation="MKAIAKYYGLLFLVALFVLAAPAEPQPLHSWEVPLRSSGRLPFH
                     LDACQFDAGDGRTRLELAYAIELAPLPGEDSLHLSIDLSLEGGQSRLAEVHEQKHFRA
                     KADTPLVYLDLKSFVLPPDTVALRMTVRDHASGRSGTAETLLPLRRFGAELSLSDLFF
                     ISSIHRPGSGDMQPFLRGGLIMLPNPSRLFGMSPSSEKAWFYFEVNHLALPAEGKGSY
                     NLRYTVSDLSGQAVRVEERPGLTIVQANSSRVESIALEGMQPGAYRLDLFLTEPGTGS
                     SAAASRYFTVSGDASGVELALPMTPADQARYLEQLSFIATRAELDLFKSLDESGKRQF
                     LIRFWQSKDPVPETPGNEYMVDYFARLAESQQRFKGGLKSDMARIYLRYGAPLDIERQ
                     TSNAGYSKPVEIWNYALNGSTQFIFVDRNNDG"
     gene            complement(1376..2674)
                     /locus_tag="PKM23_11350"
     CDS             complement(1376..2674)
                     /locus_tag="PKM23_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012776961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HNY92109.1"
                     /translation="MGAEAIRHILGRHKSGESLGLYSVCSAHRAVLHAAALQALSDDS
                     ILLVESTSNQVDQFGGYSGMKPLEFVAYVRGIAAETGLEEERIIFGGDHLGPNVWQFE
                     SAAVAMAKAHDQVVAYREAGYLKFHLDATYSCAGDPEAPLPAGLIAERTAELCQAVEV
                     GIDAGGARPLYVIGSDVPLPGGATMPGEALTVTPVAEVEEIITLTRAAFMQRGLGDAW
                     ERVIAVVVQPGVEFDHSLVHDYRPARAEALSRFIETEERLVYEAHSTDYQTGSALAEL
                     VRDHFAILKVGPWLTFAFREAVFALEMIETEWLGRRKGLQLSRLGATIEEEMAAAPKH
                     WQKHYRGTAEEQAVARRYSLSDRIRYYWPRPAVQESLGRLLANLQAGPVPAGLLSQFM
                     PEQYRAVRAGEIAAEPAAFIEHKIRQVLAAYVHATGVRRL"
     gene            complement(2664..3605)
                     /locus_tag="PKM23_11355"
     CDS             complement(2664..3605)
                     /locus_tag="PKM23_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNY92110.1"
                     /translation="MKSKRFDAIIVGEINIDLVLWRVPMPEYEKEQLAEEMRFTMGSS
                     SAITAHNLAALGARVAFVGKAGDDIFGHYMTEQLRAAGVDTSHIIMDPTLKTGATIVL
                     ANPPRKALLTYLGAMRDLVLEDIDWDFIASGHHLHLGCFFLQTGIRGEVWKLFSRARE
                     LGLTTSLDTNWDPDEEWGEDLQRALEHTDLFLPNDDEAMRIAGTKNLKEAMEHLGDKV
                     TTLVVKRGGEGAIARKGGHEVAVPPRQVKVVETTGAGDSFNAGFIFQYLQKKPLLDCL
                     EYGNACGALAVTELGGTGAFRDPAAVQTNLRKIMYGG"
     gene            complement(3615..4685)
                     /locus_tag="PKM23_11360"
     CDS             complement(3615..4685)
                     /locus_tag="PKM23_11360"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNY92111.1"
                     /translation="MELDRIQDGHTYREIVRQPAIWREALAVLAGQKREMGDFIAAHR
                     ERLWTFTGCGTSYYLAQSGAALFEKVTGLRTRAVPASEILIYPELVFNPRDQHLLVAF
                     SRSGTTTETLRAVRKAKAELKLPVLPISCDMASPLSREGDFHLTFPFPAERSVVMTGS
                     FTTMLMAILYLALQSGDHAARLAMLEKIPASCEELIGSSESRIAAIADRREADFVFLA
                     QGPYLGLANEAALKMKEMAISSSVGFHALEFRHGPMSVVTDKTMIVLLMSGPGRQLEL
                     QVAADMKKLGAEVVLMHGADRKIDASYIDHLFGVPARFGDLFNPFLFMPLLQLFGYYH
                     ARFKGIHPDRPKNLTAVVTLND"
     gene            complement(4701..>5220)
                     /locus_tag="PKM23_11365"
     CDS             complement(4701..>5220)
                     /locus_tag="PKM23_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HNY92112.1"
                     /translation="RIARAAAKLSGEGDVIILDSGSTTMAIARLLKVRKGITIITNAI
                     NIASELAASDNEIILTGGTLREKSFSLVGPLSEGSIARLTADKLFLGVDGISFECGYT
                     TPNLLEADINRLMLRAATQVIVTADSSKFGRKSLGVIAGLSAGHKIITDDHIAAGDLQ
                     RLRDLGIEVITV"
BASE COUNT         1063 a         1630 c         1563 g          964 t
ORIGIN      
        1 ggccgtcatt gttgcggtcc acaaagatga actgcgtgct gccattcagg gcgtaattcc
       61 agatctcgac cggcttgcta tatcccgcat tggaggtctg acgctcgata tccagcggag
      121 cgccgtagcg aagatatatt ctcgccatat cggactttaa tccgcccttg aaccgctgct
      181 gggattcggc cagacgggcg aaataatcga ccatgtactc gttcccgggg gtttccggga
      241 ccggatcctt cgactgccag aaacggatga gaaactgccg cttgcccgac tcatccagcg
      301 acttgaacaa atccagctca gcccgggtgg cgatgaagct cagctgctcc agatagcgcg
      361 cctgatccgc cggcgtcatg ggcagggcga gttccactcc gctcgcatcg cctgaaaccg
      421 tgaaatagcg gctggcggcc gccgatgagc ctgtgcccgg ttcggttaga aacaaatcga
      481 ggcgataggc cccgggctgc atgccctcga gggcaatgct ctccacccgt gagctgttgg
      541 cctgcacgat ggtcagtccc ggccgttcct ccaccctcac agcctgaccg gaaagatccg
      601 agaccgtgta gcgcaggttg taggagccct tgccctccgc cggcagggcg agatggttga
      661 cctcgaaata gaaccaggct ttttcgctgg atggactcat accgaaaaga cgagagggat
      721 ttgggagcat gatgaggccg ccgcgtaaaa agggctgcat atccccgctc ccgggtcggt
      781 gtatcgagga gatgaagaag agatcgctga gggagagttc cgcaccgaag cggcgcagcg
      841 gcagcagggt ttccgccgtt ccggagcggc cgctggcatg atcccgcacc gtcatccgca
      901 gtgcgacggt atcaggcggc agcacgaagc tcttgagatc cagatagacg aggggagtgt
      961 cggccttcgc cctgaaatgc ttctgctcat gcacctcggc gagtcggctc tgcccgccct
     1021 ccagggagag atcgatggac agatgcaggc tatcctctcc cggcaacggc gcaagttcga
     1081 tcgcataagc gagctccagc cgggtgcggc cgtcccctgc atcgaactgg caggcatcga
     1141 gatgaaatgg cagccgtccg ctgctccgca gtggtacctc ccacgaatgc aagggctgcg
     1201 gctcggccgg agcggccaaa acaaaaagtg caaccagaaa gagcaggccg taatattttg
     1261 caatcgcttt catggttccg ccgggatctc ctcgtcttcg caagctgtta caggctggcg
     1321 cagaggccgg gatcagccat ggtctgcggt tttccaaaat cagctccttt tcaactcata
     1381 gacgtctcac cccggttgcg tggacataag cagcgagcac ctgcctgatt ttgtgttcga
     1441 tgaaggccgc cggctcggcg gcgatttcac cggcacgcac agcccggtac tgctcgggca
     1501 tgaactggct gagcagcccc gccgggacag gcccggcctg gagattggcc agcaggcgtc
     1561 ccaggctctc ctgtacggcg ggcctgggcc agtaatagcg gatgcggtcg ctcaggctgt
     1621 agcggcgcgc cacagcctgc tcctccgccg tgccgcggta atgcttttgc cagtgcttgg
     1681 gagcagcggc catctcctct tcgatcgtag cgcccagacg ggacagctgc aggcccttgc
     1741 ggcggccgag ccattcggtc tcgatcatct ccagggcgaa cacagcctcg cggaaggcga
     1801 aggtcagcca tggcccgacc ttgagaatgg caaaatggtc gcgaaccagt tcagccaggg
     1861 cagatccggt ctgataatcg gtcgagtggg cttcatagac cagccgctcc tctgtttcga
     1921 tgaagcggct cagagcctcc gcccgcgccg gccgataatc atggacaagg ctgtggtcaa
     1981 attcgactcc gggctggacc accacggcga tcaccctctc ccaggcgtca ccaaggccgc
     2041 gctgcataaa ggctgcgcgg gtcaaggtaa tgatctcctc tacctccgcc accggtgtga
     2101 cagtcagggc ttccccgggc atggtcgccc ccccgggcag aggcacatcg gagccgatga
     2161 cgtagagcgg cctggccccg ccggcgtcaa tcccaacctc cacagcctgg caaagctccg
     2221 ccgtccgttc agcaatgagc ccggccggca gcggggcctc cggatccccc gcgcagctgt
     2281 aggttgcgtc gagatgaaac ttgaggtatc ccgcctcgcg ataggcgacg acctggtcgt
     2341 gcgccttggc catggccacg gcggcggatt cgaactgcca gacattcggc ccgagatgat
     2401 cgccgccgaa aatgatccgc tcctcctcca gtcccgtctc cgcggcaatc ccgcgcacat
     2461 aggcgacaaa ctccaggggt ttcatccctg aataaccgcc gaactggtcg acctggttgg
     2521 aggtcgactc caccagcaga atggaatcgt cactcagagc ctgaagagcg gctgcgtgca
     2581 gcaccgcccg atgcgcggaa cagaccgaat agaggcccag ggattcgccc gatttgtggc
     2641 gcccgaggat atgccggatg gcctcagccc ccatacatga tcttcctgag attggtctgc
     2701 accgcggccg gatcacgaaa agccccggtg ccgcccagct ctgtcaccgc cagggcgccg
     2761 caggcattgc cgtactccag gcaatcgagc aagggcttct tctgcaaata ttgaaagatg
     2821 aaaccggcgt tgaagctgtc tccggcgccg gtcgtctcca ccaccttgac ctggcggggc
     2881 gggacagcca cctcatggcc gccctttctg gcaatggccc cctcccctcc cctcttgacg
     2941 accagggtcg tgaccttgtc gccaaggtgc tccatcgcct ccttgagatt cttcgtgccg
     3001 gcaatgcgca tcgcctcgtc atcattgggc agaaagaggt cggtgtgctc aagggcgcgc
     3061 tgcagatcct caccccactc ttcatcagga tcccagttgg tatcgaggga ggtcgtcagg
     3121 cccagctccc tggcacggct gaagagcttc cagacctccc cccggatgcc ggtctggaga
     3181 aaaaagcagc cgagatggag atgatgccct gaagcgatga aatcccaatc gatatcctcc
     3241 aggacgaggt cgcgcatggc gccgagatag gtgagcagtg ccttgcgcgg aggattggca
     3301 aggacgatgg tggccccggt cttcagggtc ggatccatga tgatatggct ggtatcgacg
     3361 ccagcggccc ggagctgctc ggtcatgtaa tggccgaaga tgtcatcccc agccttgccc
     3421 acaaaggcaa ccctggcccc cagggcggcc agattatgcg ccgtgatggc ggaagaactg
     3481 cccatggtga agcgcatttc ctcagccagc tgctccttct catactcggg catcggcacc
     3541 cgccacaaga ccagatcaat attgatctcc ccgacgatga tggcatcaaa tcttttactc
     3601 ttcaaatcca ctccttaatc gttcaaggtt acaactgcag tcagattttt aggccggtcg
     3661 ggatgaatgc ccttgaaacg ggcgtgatag tagccgaaga gttggagaag gggcatgaag
     3721 agaaaggggt tgaagagatc accgaaccgc gcgggcactc cgaagaggtg atcgatgtag
     3781 ctggcatcta tcttgcggtc ggcgccgtgc ataaggacca cttcggcgcc gagcttcttc
     3841 atatcggcgg caacctgcag ctcgagctgg cggccgggtc cggacatcaa caggacgatc
     3901 atggtcttgt cggtgacgac cgacatcggg ccgtggcgga actcgagggc atgaaaaccc
     3961 actgaggaag agatcgccat ctccttcatc ttcagggcgg cctcgttggc caaacccagg
     4021 tagggcccct gcgccaggaa gacaaaatcc gcctcacgcc ggtccgcgat cgccgcaatc
     4081 cgggattcgc tgctgccgat cagctcttca caactcgccg ggatcttttc gagcatggcc
     4141 agcctggcgg catgatctcc ggactgaagg gcaaggtaga ggatggccat caacatggtt
     4201 gtgaaggagc cggtcatcac cacactgcgc tcggccggaa aggggaaggt gagatggaaa
     4261 tctccctccc gagagagggg cgaggccatg tcgcaggaaa tcggcagcac cggcagcttc
     4321 agttcagcct tggccttgcg cacagcgcgc agggtttctg tggtcgtgcc cgaacgggaa
     4381 aaagccacca ggaggtgctg atcacgcgga ttgaaaacca gctccggata gatgaggatc
     4441 tccgaagccg gcacagcacg cgtgcgcagc ccggtcacct tttcaaagag tgcggctccc
     4501 gattgcgcca gatagtagga ggtgccgcag ccggtaaagg tccagagccg ctcccgatgc
     4561 gccgcaataa aatcgcccat ctcccgtttc tggccggcta aaacagccaa cgcctcgcgc
     4621 caaatggccg gttggcggac gatttcccgg taggtgtggc catcctgaat ccggtcaagc
     4681 tccatattgc cacttccctt tcagactgtg ataacctcta tgcccagatc cctcaggcgc
     4741 tgcagatcac cggccgcaat atgatcatcg gtgatgatct tgtgtccggc cgacagccct
     4801 gcgatcaccc ccaggctctt gcgcccgaac ttggacgaat cagcggtgac gatgacctgg
     4861 gtcgcagccc gcagcatcag ccggttgatg tccgcctcca gcagattcgg tgtggtataa
     4921 ccgcactcaa aactgatgcc gtcgacgccg agaaagagct tgtcggcggt cagcctcgca
     4981 atggaaccct ccgagagcgg ccccaccagg gagaaagact tttcccgcag tgtgccgccg
     5041 gtgaggatga tttcattatc gctcgcagcc agctccgaag cgatattgat cgcattggtg
     5101 atgatggtga tccccttgcg caccttaaga agacgggcaa tcgccatggt cgtcgatccg
     5161 gaatcgagga tgatgacatc accctccccg ctgagcttgg ccgccgcccg ggcgatccgc
//
LOCUS       DAOPWU010000247         5216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15914_length_5216_cov_4.293354, whole genome shotgun sequence.
ACCESSION   DAOPWU010000247 DAOPWU010000000
VERSION     DAOPWU010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15914_length_5216_cov_4.293354"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PKM23_11370"
     CDS             complement(<1..809)
                     /locus_tag="PKM23_11370"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNY92113.1"
                     /translation="MSKLWEEYLIQEDLIGHSPAIYQIRRSIPILAKGDQPVLITGEP
                     GTEKLLIAKLVIDNSARKKAPVFITDAAKLNKTFDQEIQSLLQQQEHSSDDGLRGTLI
                     VQNLEYLDRESQARLMSFAQGGYLQLQPHAEPMAAEFRIISTATSKLQEYAKDGIFSS
                     ELYLTLSAMTVKLPALRERKQDIVAFFEFYLDYFCRKIGRPVPPMPFEIFHQILKYSW
                     PGNVKEMENAVRSVVVSSPEGMLLMEALPFVDEAEKFQRVELQSLNRAVAQL"
     gene            complement(1087..1458)
                     /locus_tag="PKM23_11375"
     CDS             complement(1087..1458)
                     /locus_tag="PKM23_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92114.1"
                     /translation="MKHFIALLAMITFLFFLSLSVNIAQDEALRLNRMEMESMLNLLD
                     HSAVKGSDIDGLADLCAKLRAGARQVALFPDSSRTVVLKVTPREAKICLGMIENATIQ
                     ARNAEMVQRIRIKLLHYASSV"
     gene            complement(1639..1932)
                     /locus_tag="PKM23_11380"
     CDS             complement(1639..1932)
                     /locus_tag="PKM23_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92115.1"
                     /translation="MRPTFWSEPDLMRKARSQGWRLLAGSDPLPFKGEERSFGRYGCM
                     ITGEMDPEEPILSLKRLLVEQGIEPTIWGQRRGTWEFAKRQLAIMYEKKRREG"
     gene            complement(1899..2438)
                     /locus_tag="PKM23_11385"
     CDS             complement(1899..2438)
                     /locus_tag="PKM23_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92116.1"
                     /translation="MSEKSLIIDAHIHLYPQADPAQALAFSLMNMDAARPEKEAAKFW
                     LLTERSDCSAFSQLQESVRIGLYHIIPTREQEALRIQLGERIVLFIIAGQQVVSADGL
                     EIGTLASSLRLPDRELETSVLIDRALEAGSLVTINWAPGKWSGRRKEIVRSLLSSCRG
                     GAFSSGTAPCAPPSGPNPT"
     gene            complement(2523..3677)
                     /locus_tag="PKM23_11390"
     CDS             complement(2523..3677)
                     /locus_tag="PKM23_11390"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNY92117.1"
                     /translation="MMCPELLQAFQRRFGSTPRIFRAPGRVNLIGEHTDYNDGYVFPM
                     ALDRYTAVAVAPRSDRMVRVYSASMEEEITFPLDNPRRRGHWSDYIAGVASQFEMRKV
                     RLPGADLLLFSDVPVGSGLSSSAALEISSALAFLSLTDDQLAGEELARLGQSAENQFV
                     GMNCGIMDQFIAVHGARDHALFLDCRSLEFQLVPLPSDHCRVVVCNTMVKHELGASEY
                     NTRRRECEEGVHHMQKQWSTVKALRDLTLEQFAHVEEELDPIVRKRCRHVISENERVL
                     DSIRSLRRGDLHHFGELMNSSHDSLRDDYQVSCEELDLMVGIARGIPGCLGARMTGGG
                     FGGCTVNLVETDAVDLFCREVGALYRNRTGLAPAIYISVPSQGAHEWNSQ"
     gene            complement(3757..3975)
                     /locus_tag="PKM23_11395"
     CDS             complement(3757..3975)
                     /locus_tag="PKM23_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92118.1"
                     /translation="MSSSKMYSIWFFVGVMLTILGLIIVSMGVYYIFSPETHTRLASL
                     NPSLWWGAIMLTAGLLFLIPSWIEHRKG"
     gene            complement(4028..>5216)
                     /locus_tag="PKM23_11400"
     CDS             complement(4028..>5216)
                     /locus_tag="PKM23_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/galactose cotransporter"
                     /protein_id="HNY92119.1"
                     /translation="GCYIMLGGLMSAIFTEIVQFFLIWFGLFLVAILGVVEMGGINQV
                     LTGLPDAMSKLWSTTGSAANNAMGVTWAYIVFGLGWVLSFGYWTTDFLVVQRAFSSKD
                     LRSARMTPIIASFFKMALPFIIVIAGLVAYKLTQMAGADFKLLAHPVTGEPWNDTALP
                     LLIARYYPQGLVGLGITALLAGFMAGQAGNVSAFNTVWTYDIYRVLINKNASDAHLVW
                     MGRITTLVGILLSVATAYLAMQFNTIMDYIQAIFSWVNAPLFATMLLGMFWKRTSPTG
                     AFWGLILGMVTSFTIFLGFKMDFLSTGMVNLITFSNAPVSEISDMSRNLSQAAWAWSV
                     CAIATVLISLFTKPKPEAELVGLVKGFTTIEHEGKIAWYSKPEYIAVIAVVVFVILNI
                     YFW"
BASE COUNT         1113 a         1373 c         1693 g         1037 t
ORIGIN      
        1 agctgcgcga cggcgcgatt gagactctgg agctcgacac gctggaactt ttccgcctca
       61 tcgacaaagg gcagggcttc catcagcagc atgccctcgg gcgaggagac cacgacgctg
      121 cggacggcgt tctccatctc cttgacattc cccggccagg agtatttgag gatctggtga
      181 aagatttcga agggcatggg gggaaccggg cggccaatct tgcggcagaa atagtcgaga
      241 taaaactcga aaaaggcgac gatatcctgc ttgcgttcgc gcagggcggg aagtttaacg
      301 gtcatggcgc tgagggtgag gtagagctca gagctgaaaa tcccatcctt ggcatattcc
      361 tgcagcttgg aggtggcggt ggaaatgatg cggaattcgg cggccatggg ttcggcgtgc
      421 ggctggagct gaaggtatcc cccctgggcg aagctcatca gccgcgcctg ggattcacgg
      481 tcgagatact ccaggttctg gacgatgagc gtgccgcgca gaccatcgtc ggaggaatgc
      541 tcctgctgct gcaggaggga ctggatttcc tggtcgaaag tcttgttgag tttggcggcg
      601 tcagtgatga agaccggcgc ttttttgcgg gcgctgttgt cgatgacgag tttggcgatc
      661 agcagttttt cggtgccggg ttcgccggtg atcagaacgg gctgatctcc cttggcgaga
      721 atcggtattg agcggcggat ctgatagatg gccggagaat gtccaatcaa atcctcctgg
      781 atgaggtact cctcccagag tttgctcata gggtgcttcc cttaaatatg ccgacgcata
      841 agatgccaat atctattcag aacagtgtgt tagccgctca gatgttgtcg taggaaaggt
      901 tgggtggaga gtgagtgtca caagagtatt acacttctat ccctattaat attatctctt
      961 ttacggccaa aaagcaagga aaaaataaca aaataaaata attttcggga taaaaaaaac
     1021 cggcctgtca gaatggagat gtgacaagcc ggtccctgtg aggagacaca gttcaaaccg
     1081 aatatttcag accgacgagg cgtagtgcag gagtttgatc cggatgcgtt ggaccatttc
     1141 ggcgttgcgg gcctggatgg ttgcattctc gatcatgccc aggcagattt ttgcctccct
     1201 gggggtcacc ttgagcacta cagtccgaga ggaatccgga aagagggcta cctgccgggc
     1261 gcccgcgcgc agttttgcgc agagatcggc cagaccgtcg atatcggagc ccttgacagc
     1321 ggaatggtcg agcagattga gcatgctttc catttccatc cggttgagcc gcagggcctc
     1381 atcctgagcg atgttgacgc tgaggctcag gaaaaagaga aaggtgatca tggccagcag
     1441 ggcgataaag tgtttcatcc agcctcctgt cggaactaca aatttcttgc agcgacactt
     1501 tgcctattgc aagcagcatg ccaggaatgc gaggcagttt tgtgcggggt tctccctgac
     1561 gcggcggctg agtggggttt ccgcgccaca gctccggcgc cgggtgtggc gcactctata
     1621 cactccccgg agtggtgttc atccctccct gcgcttcttt tcatacatga tcgcgagctg
     1681 cctcttggca aactcccagg tgccgcgtct ttggccccag atggtgggtt cgatcccctg
     1741 ctccaccaaa agccgtttca gcgagaggat gggttcttcg gggtccatct ccccggtgat
     1801 catgcacccg tagcgtccga aagagcgttc ctctcccttg aaggggagcg ggtcagaacc
     1861 cgccagcaga cgccatccct ggcttcgggc cttgcgcatc aagtcgggtt cggaccagaa
     1921 ggtggggcgc atggcgctgt ccccgatgaa aaggccccgc cgcggcagct gctcagaagg
     1981 gaacgcacaa tctccttgcg gcgtccggac cattttcccg gcgcccagtt gatcgtgacc
     2041 aggctgccgg cctcgagggc cctgtcgatg agaacggagg tctccaactc gcggtcggga
     2101 agtctgagac tagaggcgag ggtgccgatc tcgagcccat cggcgctcac cacctgttgt
     2161 ccggcgatga tgaaaaggac gatgcgttcg ccgagttgga tgcgcagggc ctcctgctcc
     2221 ctggtcggga tgatgtgata gaggccgatg cggacgctct cctgcagctg gctgaatgcg
     2281 gagcagtcgg agcgctcggt caggagccag aatttggcgg cctctttctc cggcctggcc
     2341 gcatccatat tcatcaggct gaaggcgagt gcctgggccg gatcggcctg gggatagagg
     2401 tgaatatggg catcaataat cagggatttc tcagacatgg ctcctcacaa tggcgaggcg
     2461 gggccgatgg agtccgggtg aagagtcggg cagggccgcc tttcgcacgc taaactgatg
     2521 gtttactggc tgttccattc gtgggccccc tgtgagggca ccgagatgta gatggcggga
     2581 gcgaggccgg ttctgttccg atagagcgct ccgacctccc ggcagaagag gtctaccgca
     2641 tcggtttcga ccagattgac ggtgcagccg ccgaatcctc ccccggtcat gcgggcgcct
     2701 aggcagccgg ggattcccct ggcgatcccg accatgagat cgagttcctc gcagctgacc
     2761 tgataatcgt cacgcaggct atcatgggag ctgttcatca gttcgccgaa gtggtgcaga
     2821 tcccctctgc gcaggctgcg gatcgagtcg aggacgcgct cattctcgct gatgacgtga
     2881 cggcagcgtt tgcgcacgat ggggtcgagt tcctcctcaa catgagcgaa ctgttcgagg
     2941 gtgagatcgc gcagggcttt gacggtggac cactgttttt gcatatggtg gaccccctcc
     3001 tcgcactccc ggcgtcgggt attgtattct gaggctccaa gttcatgctt gaccatggtg
     3061 ttgcagacca cgacgcggca gtgatcggag ggcaggggga cgagctggaa ttcgagggag
     3121 cggcagtcaa gaaagagggc atgatcgcgg gcgccatgga ccgcgatgaa ctggtccatg
     3181 atgccgcaat tcatgccgac gaactgattt tcagccgact ggcccaggcg ggccagttct
     3241 tctcctgcaa gctgatcgtc ggtcaggctc aggaaggcga gcgcggagga gatctccagg
     3301 gcggccgaag agctgaggcc ggaacccacc ggcacatcgc tgaagagcag aaggtccgcg
     3361 ccgggcaggc gcacctttct catttcgaat tggctcgcga caccggcgat atagtcgctc
     3421 cagtggccgc ggcgccgggg attgtcgagg ggaaaggtga tctcctcctc catgctggcc
     3481 gaatagaccc ggaccatgcg gtcgctgcgc ggcgcgaccg ctacggcggt gtagcggtcg
     3541 agggccatag gaaagacgta gccgtcgtta tagtcggtat gctcgccgat gaggttgacc
     3601 cgtccgggcg cccgaaaaat gcggggcgtg gagccgaacc gccgctggaa ggcttgcaac
     3661 aattcagggc acatcatgcg caggtaatcc tttatgaaag aacagacgag gcgaagcgga
     3721 taggcgctgc gccccgtctg tccggtgcac gtgtgatcag cctttccggt gctcgatcca
     3781 ggaggggata aggaagagca gtccggcggt caacatgatg gcgccccacc acagggaggg
     3841 attcagactg gcgaggcggg tatgtgtttc cggagaaaag atgtaataga cacccatgga
     3901 gacgataatc agccccagga tcgtgagcat gacccccacg aaaaaccaga tggaatacat
     3961 cttgctgctg gacataggca ctccttgtca agggtgggcc gcccgtgagg gcagcggtga
     4021 acaggattta ccagaagtaa atgttgagga tcacgaaaac cacaaccgcg atgacggcga
     4081 tgtactcggg tttgctgtac caggcgattt tgccttcgtg ttcgatggtg gtaaaacctt
     4141 tcaccaggcc gaccaattcc gcctccggct tgggtttggt gaaaagggaa atgagaaccg
     4201 tggcgatggc gcacaccgac caggcccagg cggcctggga gaggttgcgg gacatgtcgg
     4261 agatttcgct gactggcgcg ttgctgaagg tgatgaggtt gaccatcccc gtggacagaa
     4321 agtccatttt gaagcccagg aagatggtaa atgaagtgac catgccaaga ataagccccc
     4381 agaaagcgcc ggttggggag gtgcgcttcc agaacatgcc gagaagcatg gtggcaaaga
     4441 ggggcgcatt gacccagctg aagatggcct ggatatagtc catgatggtg ttgaactgca
     4501 tggccaggta ggcagtggcg acggagagaa ggatgccgac cagggtggtg atgcgtccca
     4561 tccagaccag gtgggcgtcg ctggcgttct tgttgatgag gacgcggtag atgtcatagg
     4621 tccagacggt gttgaaagcg gagacattgc cggcctgccc ggccataaag ccggcgagga
     4681 gggcggtgat gccgagaccg accagtccct gcggataata gcgggcgatg agcagcggca
     4741 gggccgtgtc attccagggc tccccggtca caggatgagc cagtagtttg aaatcggctc
     4801 ctgccatctg ggtgagcttg taggcgacga ggccggcgat gacaataata aaaggcaggg
     4861 ccattttgaa aaagctcgcg atgatcgggg tcatgcgcgc cgagcgcaaa tcttttgagg
     4921 agaaggcgcg ctgcaccacc agaaagtcgg tggtccagta gccgaaggag agcacccatc
     4981 ccagtccgaa gacaatgtag gcccaggtca ctcccattgc attattggcg gccgaaccgg
     5041 tggtggacca cagcttgctc atggcatcgg gcaggccggt gagaacctga ttgatcccgc
     5101 ccatctcgac gacgccgagg atggcgacca ggaagaggcc gaaccagatc aggaagaact
     5161 ggacgatttc ggtgaagatg gcggacatca gccccccgag catgatgtag cagccg
//
LOCUS       DAOPWU010000248         5208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15946_length_5208_cov_4.008733, whole genome shotgun sequence.
ACCESSION   DAOPWU010000248 DAOPWU010000000
VERSION     DAOPWU010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15946_length_5208_cov_4.008733"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKM23_11405"
     CDS             <1..275
                     /locus_tag="PKM23_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92120.1"
                     /translation="AKPSPEQFTQSIIDKFTRELSLNEGQIEALREQLQQLRTRYDSL
                     RVQNATCSAQIREQFRAEFSKVLTPEQQIRFVEFNKREDEKHWGKK"
     gene            328..1692
                     /locus_tag="PKM23_11410"
     CDS             328..1692
                     /locus_tag="PKM23_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNY92121.1"
                     /translation="MKKWILAAAGIVLLLLIWFLTCSDKKAPAAMTTLPQTDKVMRGD
                     LRVEVSASGVVEPINKVEIKSKASGQIVEMQVEESDPVLRGDLIARLDQLDTRNSYNT
                     AKADLEVAEANVKQKQSDFDRKAELYEKGLLSAADFDNAKLALVEAKAQVVRSRINLD
                     NADIKLKETIVRSPIDGVVLTKDVEVGQIIASGISSVSGGTLIATVANMQRVYVKADV
                     DEVDIGVIQPGMPSTVVADAYPDKVFNGKVIRIAAQGKVVSNVTTFEVTIEVENPESK
                     LKAGMNTSVDILVADKRDVLLVPNEALMSRKELQQELAKLKLLTDPTAKANSVAQGTP
                     MAGGPDRRGGGMRREGGGRPGATGSATAAGADPGKGQPQIEHIGGGEETRRGVILQKG
                     QEYEIRLVKTGVSSLDHTEVLAGLAENDQVVYTFYSRATEASTQMRERMTAMQSQRSG
                     FRSN"
     gene            1724..2956
                     /locus_tag="PKM23_11415"
     CDS             1724..2956
                     /locus_tag="PKM23_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY92122.1"
                     /translation="MKKKLFSEFSESLKIALSSLAANKMRAILTMLGIIIGVAAVIAM
                     VGVGSGAQKAIADRIQAMGSNLLFVQPGSSRQGHIHFGSGSMITLMPEDVDALRENSR
                     YIAAVLPEFNRRAQAQFGNKNWNTSIVGTVPEYCDVRNVKVTSGRMITRDEVENTERV
                     AVIGTEVQSNLFGSASPVNQTIRIAQDNFVVVGVFEKKGQQGWQNQDDQIVIPYTTAQ
                     RRVFGAEYLTGITVKVIDQAYVQDALIEVEKILRRQHRLNRDQDNDFSIRNQSDIIST
                     MQETNKTFNFLLAAIAGVSLIVGGIGIMNIMLVSVTERTREIGIRKAIGARRRDIMMQ
                     FLVESIMLSLAGGTIGILVGVLASFLLATLAHWNTLISPASILLSFGFSTAVGLFFGL
                     YPARKASMLDPIIALRYE"
     gene            3143..4675
                     /locus_tag="PKM23_11420"
     CDS             3143..4675
                     /locus_tag="PKM23_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNY92123.1"
                     /translation="MKYLSKSASSLLFIVPALLLFSAGFLQIAHAQKAELYEVLFGEK
                     DKQLKSYKNSQADLLSPGHYARAVEAYAKAKEEFKKGKDTGEVRKRLAEVEAAFQQID
                     NTLWSGKGLFDEVLKAREDALNARAPEFALEDFNAAEELLRSAGSAQERADQDLTRER
                     SIKARAKFRETELNAIKKSIMTPAHEALNAALKAKADKSAPKSYNQARELLTLAEEIL
                     NSNRYAVSDATAKAEEAIYQARHAVKITELLQSRKFSQEELVLLYETGLTQVAKELGY
                     DAAFDQPIAEIINTMATSVRSLQEENKQLESDLQEKQELLDKAQQAIEAERAKAKKQI
                     EALAARSETDIALAQQKESALQDELAMKEAELLKKQQKEARIAKIRRMFLPEEAKVLI
                     EENHLIIRLMGLTFPIGKAVIQPEYFGLLSRVQRGIREYPDAKIVIEGHTDSSGDERY
                     NERLSTKRAEAVRAYILSNMVLAADQIDALGYGESRPVANNDTEEGRAQNRRIDVLIA
                     ID"
     gene            4779..>5208
                     /locus_tag="PKM23_11425"
     CDS             4779..>5208
                     /locus_tag="PKM23_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92124.1"
                     /translation="MKRGILVLVSFTLVILLNSAFAADKIVPQIRQGDKALLFTINGL
                     GSFGVNGANAGLLVEENSGDVESSALYGLGFKTFISNRIALRVGLCYMGASVTTETDD
                     GDEVESQSMLGLQPAIEYHLFQSEAVSIYTGGMISFSSYSA"
BASE COUNT         1182 a         1558 c         1498 g          970 t
ORIGIN      
        1 gcgccaagcc ctctcccgag cagttcacgc aaagcatcat cgacaagttc acccgagaac
       61 tctctctgaa cgaaggacag atcgaagcct tgcgcgagca gcttcaacag ctgcgtacgc
      121 gctatgattc cctgcgcgtg caaaacgcta cctgctcggc gcagatcagg gagcagttcc
      181 gcgcggagtt cagcaaggtt ctcacccccg agcagcagat ccggtttgtg gagttcaata
      241 aacgcgaaga tgaaaaacat tggggcaaga aataaggccc gatcatccat gaatagttca
      301 cctaaaacaa gtggagccct taactaaatg aagaaatgga ttttggcagc agccggaatc
      361 gtactgctgc tgctcatctg gttcctgacc tgcagcgaca agaaggcgcc ggcggccatg
      421 actaccctgc ctcagaccga caaggtgatg cgcggcgatc tgcgggtgga ggtctcggcc
      481 agcggagtgg tcgaaccgat caacaaggtg gagatcaaga gcaaggccag cggccagatc
      541 gttgaaatgc aggttgagga gtcagatcca gtcctccggg gtgatttgat cgcccgcctg
      601 gatcagctcg acacgcgcaa ctcctacaat acggcaaaag ccgatctcga ggtcgccgag
      661 gccaatgtca agcagaaaca gagcgatttt gaccgcaagg ccgagctgta tgaaaagggg
      721 ctcctctccg cagccgattt cgacaacgcc aagctcgccc tggtcgaagc caaggcgcag
      781 gtggtgcgca gccggatcaa cctcgacaat gccgacatca agctcaagga gaccatcgtc
      841 cgctccccca ttgacggcgt tgtactgacc aaggatgtcg aggttggaca gattatcgcc
      901 tccggtatct catcggtctc cggcggcacc ctgattgcca cggtggcgaa catgcagcgg
      961 gtttacgtca aggccgatgt tgacgaggtc gatatcgggg tcattcagcc cggcatgccc
     1021 tccacggtag ttgcagatgc ctatccggac aaggtcttca acggcaaggt gatccgcatc
     1081 gccgcccagg gcaaggttgt ctccaatgtc accacttttg aggtcaccat cgaggtcgag
     1141 aatcccgaat cgaagctcaa ggccggcatg aacacctcgg tggatattct tgttgccgat
     1201 aaaagggatg tgctgctggt gccaaacgag gccctgatgt cccgcaagga gcttcagcag
     1261 gaactcgcca agctcaagct gctcaccgat ccgacggcca aggcgaacag cgtggcacag
     1321 ggcactccga tggcgggcgg cccggatcgc cgcggtggcg gcatgcgcag ggagggcggc
     1381 ggccggccgg gcgcaacggg ttcagcgacc gctgccgggg ctgatcccgg caaaggccag
     1441 cctcagatag agcatatcgg gggcggcgag gaaacgcgcc gtggtgtcat cctccagaaa
     1501 gggcaggagt atgaaatccg cctggtcaag accggcgtca gcagtcttga ccacactgag
     1561 gtgcttgcag gtctggctga gaatgaccag gtggtctaca ctttctattc ccgggcgacc
     1621 gaagccagca cccagatgcg tgaacggatg accgcgatgc agagtcagcg cagcggcttt
     1681 cgcagtaatt aacggggcaa gcccttcaca gcgaggaatt ccagtgaaga aaaagctgtt
     1741 ttcagagttc agcgagagcc tcaagatcgc tctcagctcc ctggcggcca acaagatgcg
     1801 tgccattctg accatgctcg gcatcatcat cggcgtggcc gcggtcatcg caatggtcgg
     1861 ggtcggctcc ggcgcgcaaa aggctattgc cgaccgcatc caggccatgg gctccaatct
     1921 cctcttcgtc cagcccggct catcgcggca gggacacatc cacttcggct ccggcagcat
     1981 gatcaccctc atgcctgagg atgtcgatgc cctgcgggag aacagccgct acatcgcagc
     2041 cgtacttccg gaattcaacc gccgggccca ggcccagttc ggcaacaaga actggaacac
     2101 ctcgatcgtc ggcaccgtgc ctgagtactg cgatgtacgc aacgtcaagg tgaccagcgg
     2161 acgtatgatc accagggatg aagtcgaaaa taccgagcgg gttgccgtga ttggcaccga
     2221 agtgcagtcc aacctcttcg gcagcgcctc gcccgtcaac cagaccatcc ggatcgccca
     2281 ggacaatttc gtcgtcgtcg gtgtctttga gaaaaagggg cagcagggtt ggcagaatca
     2341 ggatgaccag attgtcatcc cctacaccac ggcccagcgc cgcgttttcg gcgctgaata
     2401 tctcaccggc atcacggtga aggtcatcga tcaggcctat gttcaggatg ccctcatcga
     2461 ggtcgaaaag atcctgcgcc gccagcaccg gctcaaccgg gatcaggaca atgatttttc
     2521 gatccgcaac cagtcggaca tcatcagcac catgcaggaa accaacaaga cctttaattt
     2581 tctcttggcg gcgatcgccg gggtctcgtt gatcgtcggc ggcatcggca tcatgaatat
     2641 catgctggtt tcggtcacgg agcggaccag ggagatcggc attcgcaagg cgatcggcgc
     2701 acgccggcgc gacatcatga tgcagtttct ggtcgagtcc attatgctga gcctcgccgg
     2761 cggcacaatc ggcatcctcg tcggggtgct ggcctccttc ctgctcgcca cactcgctca
     2821 ctggaacacc ctgatctctc cggcttccat tctgctcagc tttggttttt ccactgcggt
     2881 cggcctcttt ttcggactct acccggcgcg caaggcctca atgctcgatc ccatcatcgc
     2941 attacgctac gaataagcac tctcccgccg gttcctgccg gcgggtgttc tcttctccca
     3001 ccgttctgct gtatcggccc gctccatatt attcctgacg ctttatggcc attctgcttg
     3061 cttttcttgc catcccgccg tatatttcag cagcccttcg gcccgcaggg gtcagtcatt
     3121 ccaaccaccc gaggaaaatt atatgaaata tttgtccaaa tccgcctcct ccctcctgtt
     3181 cattgtgccg gccctgcttc tcttctccgc cggatttctg cagatcgccc atgcccagaa
     3241 ggcagagctc tacgaggtcc tcttcgggga gaaagacaag cagctcaagt cctacaagaa
     3301 cagccaggcc gatctgctca gccccggcca ttacgcacgt gcggtcgaag cctatgccaa
     3361 ggccaaggag gagtttaaaa agggcaagga taccggcgag gtgcggaagc ggctggctga
     3421 ggtcgaggcc gccttccagc agatcgacaa taccctctgg tccgggaagg ggctttttga
     3481 tgaggtgctc aaggcccgcg aggatgccct caacgcccgc gccccggaat tcgccctcga
     3541 ggatttcaac gccgcagagg agctgctgcg ctctgccggc tcggcgcagg agagggcgga
     3601 tcaggatctc acccgcgaaa ggagcatcaa ggccagggcg aaattcaggg agacggagct
     3661 caatgccatc aaaaagagca tcatgacacc ggcccacgag gctctcaacg ccgccctcaa
     3721 ggccaaggcg gataaatcgg cgcccaagag ctataaccag gcccgcgagc tgctcaccct
     3781 cgccgaggag atactcaaca gcaaccgcta cgcggtctcc gacgccaccg ccaaggctga
     3841 ggaggccatc taccaggccc gccatgccgt caagatcaca gaactgctgc agtcgagaaa
     3901 attctcccag gaggagctgg tgctcctcta tgagaccggc ctgacccagg tagcaaagga
     3961 actcggctat gacgcagcct tcgaccagcc catcgccgag atcatcaata ccatggccac
     4021 ttcagtgcgg agtttgcagg aggaaaacaa gcagctcgag agcgacctgc aggaaaagca
     4081 ggaactgctg gataaggcgc agcaggcgat cgaggctgag cgagcgaagg ccaaaaaaca
     4141 gattgaggcc ctcgcggcca ggagcgagac cgatatcgcg ctggcccagc aaaaggagag
     4201 cgctctgcag gatgaactgg ccatgaagga ggctgagctg ctaaaaaagc agcagaagga
     4261 ggccaggata gccaagatcc gcaggatgtt cctgccggag gaggccaagg ttttgatcga
     4321 ggagaaccat ctcatcatcc ggctcatggg ccttactttt ccaatcggca aggcggtcat
     4381 tcagccggaa tatttcggtc tgctctcccg cgtccagcgc ggcatccgcg aatatcccga
     4441 tgccaagatt gtcatcgaag gtcatactga ctcgtccggg gatgaacgct acaacgagcg
     4501 tctctcgacc aaacgggcgg aggcggtgcg cgcctatatc ctctccaaca tggttttggc
     4561 ggcggaccag atcgatgcct tgggatacgg cgagagccgt ccggtggcca ataacgatac
     4621 cgaggagggg cgcgcccaga atcgcaggat tgatgtcctg atcgccatcg actagatcaa
     4681 acaaggataa acccggacgc gatgccggta ccatcgcgaa tacttattat cgcagcagtg
     4741 accatgcaag tcaaccatct gagaacaagg aggaagtgat gaaaagaggg attctggttc
     4801 tggtcagttt taccctggta atcctgctga actcggcttt cgcggcggac aagatcgtcc
     4861 cccagatcag gcagggtgac aaggcgctgc ttttcaccat caacggactg ggctccttcg
     4921 gggtcaatgg ggcgaacgcg ggcttgctcg tcgaggaaaa cagcggcgat gtcgagagtt
     4981 cggctctcta cggcctcggt tttaaaacct tcatctccaa ccggatcgcc ttgcgcgtgg
     5041 gactgtgcta catgggcgcc tcggtgacca ccgaaaccga tgacggggat gaagtggaga
     5101 gccaatccat gctcggcctg cagccggcga tcgaatacca tctttttcag tcggaagccg
     5161 tctccattta caccggcggc atgatctcat tcagctccta ctccgcca
//
LOCUS       DAOPWU010000249         5205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_15963_length_5205_cov_6.289126, whole genome shotgun sequence.
ACCESSION   DAOPWU010000249 DAOPWU010000000
VERSION     DAOPWU010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15963_length_5205_cov_6.289126"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PKM23_11430"
     CDS             <1..1209
                     /locus_tag="PKM23_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY92125.1"
                     /translation="PDESTQAAARPAAEPDLSLAEMGFEYAAGAGEKAAPVEEELLDL
                     PPEIVPLEAVAQAEAGNVESNLPERNIEPPLPEPGLELRDDEDDLTSDDLDFADILET
                     GGGLEDPFALREEGRRFPAAPMEEAVVLPAGESEREDFSPPQEDADPALRDIFEEFLE
                     IDEEAGLISESGELENEIIEQILQNEIASRMNWQVGEQEGAGPDLAGQWQLEEAAGEV
                     SPGEEEVKDQHRLSFDELIDLLSAEKNGGKEWPEPGLEAAERARSSEGGGSAAAPAHA
                     DDPDRDRAYANALSRFLNEEYRQSIEICSEILARDGGDTQVRVLLGQSYFRANAFRES
                     AREFQRILKIDPRNKEAHEKLGVIYANQGAFPRAIAHWEYLLQLAPERRDIFESIQRA
                     KQFINQTESS"
     gene            1397..2059
                     /locus_tag="PKM23_11435"
     CDS             1397..2059
                     /locus_tag="PKM23_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY92126.1"
                     /translation="MEGIQLSVEKTGTKNSVSIIKVGGYIDTTTSSELEHALSTLLKA
                     GSYNIIIDLGNVDYVSSAGWGIFISEIKGIREKGGDLKLVRMIPEVYEVFELLEFHYI
                     LKACDSIDEAIKDFERTLGPVTEGSKVAEVDMEAAHSRGGGERRESAPRPESGRADNL
                     EEKIRKIIGQDPEIGTIQLIRTLNTPEHGNIRLGWFAMRKLLKKSGLDSKKGRALLSS
                     GR"
     gene            2162..3154
                     /gene="obgE"
                     /locus_tag="PKM23_11440"
     CDS             2162..3154
                     /gene="obgE"
                     /locus_tag="PKM23_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNY92127.1"
                     /translation="MFIDHAKIRVQAGSGGAGCVAFRREKYVPKGGPNGGDGGHGGDV
                     IAIADRHIHTLLDFKYQTLFKAERGQHGMGSNMTGKSGQPLLIRMPIGTTIRNAESGE
                     LIADLTKEGEEAVIARGGRGGRGNSRFVTPTNRTPREWDPGELGEELELTLELKLIAD
                     VGLVGLPNAGKSTLLSRISAARPKIADYPFTTLIPNLGIVRVSDAQSFVVADIPGLIE
                     GAHEGKGLGIQFLRHIERTRILAILLDCSSAEMEADYKTLINELKSYSPDLLRKKRLL
                     IYTKKDLVQGRIKPLKARLRGDAPEITISAVRGDGLEELIRLIWDTLQQDDQAD"
     gene            3176..4267
                     /gene="dprA"
                     /locus_tag="PKM23_11445"
     CDS             3176..4267
                     /gene="dprA"
                     /locus_tag="PKM23_11445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNY92128.1"
                     /translation="MESATPELLSLWNTPGIGPQRLRSLIERFGSGRAVYAASTRQLC
                     EVEGIDQATAAKIKANRDLEGATRELERTRKAGVTVLSLWDAAYPEMLRPLYDLPVIL
                     FVQGDLEALSARAVAVVGTRVPSEYGQIVARKLTRELVQRGLVIVSGLARGIDTIAHH
                     EAVTQGGRTVAVLGSGLDQIYPGENRRLARMITEHGALISEYLLGTQPDAPHFPRRNR
                     IIAGLAAATLVVEAGEGSGALITADDALEYNREVMAVPGNITNPRAYGCNHLIQQGAK
                     LVQGVEDILVEIGMAEEPPQSRQLELPGVGISADEEKLLGVLSQEPRHVDEIAQVLGR
                     PVSTVLAQLLELELKSLVNQLPGKKFVRA"
     gene            4341..4424
                     /locus_tag="PKM23_11450"
     tRNA            4341..4424
                     /locus_tag="PKM23_11450"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4375..4377,aa:Ser,seq:tga)
     gene            4461..4549
                     /locus_tag="PKM23_11455"
     tRNA            4461..4549
                     /locus_tag="PKM23_11455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4495..4497,aa:Ser,seq:gct)
     gene            4615..>5205
                     /locus_tag="PKM23_11460"
     CDS             4615..>5205
                     /locus_tag="PKM23_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNY92129.1"
                     /translation="MAKTRVRFAPSPTGFVHIGSLRTALYNYLYARNQGGDFILRIED
                     TDRERFVEGAVENLISVLEWAGLRPDEGPRWGGACGPYFQSERTETYRHYAQELLKKG
                     YAYYSFDTPADIEAMRARAKAAGNPYAKYNFANRMEMRNSLSLPPAEVEEAIADGTPY
                     AIRLLVPEGRKFEVEDEVRGHVVFDSKEVDDQVLLKS"
BASE COUNT         1073 a         1488 c         1673 g          971 t
ORIGIN      
        1 cctgatgaat ccactcaggc ggccgccaga ccggccgccg agcccgatct ttcccttgcg
       61 gagatgggtt ttgaatatgc cgccggcgct ggtgaaaagg cggcccccgt cgaggaagag
      121 ctgctggatc tgcccccgga gatcgttccc ctcgaggcgg ttgcacaagc tgaggccggt
      181 aacgtggaga gcaatctgcc cgaaaggaat atcgagcccc ccctgccgga gccggggctg
      241 gagctgcgtg atgatgaaga tgatctcacc agcgacgatc tcgattttgc ggatattctc
      301 gagaccgggg gcgggcttga ggatcccttc gcgcttcgcg aggagggcag gagattcccg
      361 gccgcgccga tggaagaggc ggtcgtcctt cccgccggcg agtcggagag agaggatttc
      421 agcccaccac aggaagatgc cgatccggcg ctgcgcgaca tctttgagga gtttctcgag
      481 atcgatgaag aggccggcct gatttccgaa agtggcgagc tggagaacga gatcatcgag
      541 cagatattgc agaacgagat cgcctcgcgg atgaactggc aggtcgggga gcaggagggc
      601 gccgggccgg acctggcggg gcaatggcag ctggaggagg cggccggcga ggttagtcct
      661 ggcgaggagg aggtaaagga ccagcatcgc ctctcttttg acgagctcat cgatctgctc
      721 agcgccgaga agaacggcgg gaaggagtgg ccggagccgg gcctcgaggc ggcggaacgg
      781 gcgaggagtt cggagggggg aggaagtgcc gcggcgccgg cccatgctga tgatcccgac
      841 cgcgaccgcg cctacgccaa tgccctcagc cgttttctga acgaggagta caggcaatcg
      901 atcgaaatct gctcggagat tctggcgcga gacggcggcg atacccaggt gcgcgtgctg
      961 ctggggcaat cctatttccg cgccaacgct ttccgtgaat cggcgcggga gtttcagcgc
     1021 attctcaaga tcgatccacg caacaaggag gcgcatgaga aactgggggt gatttacgcc
     1081 aaccaggggg cgtttccgcg ggccattgcc cattgggaat atctgttgca gctcgcgccg
     1141 gaacgccgcg acatttttga aagcatccag agagccaaac agttcatcaa ccagaccgag
     1201 tcatcctgac cctgggcgcc cctgacttgc tggatatttt tgttgactat tagaggcata
     1261 aaggctatat ttgtctgctg gaagaactcg tccggacggg gtatctggtg ggatggcgtg
     1321 ctttcgactc gacattgcgg ttacagtctt gcgcccagtt cctgcaatcg cgaaataatc
     1381 ttgatggaga ttcaaaatgg aaggcatcca attgtcagtg gagaagacgg gaactaaaaa
     1441 cagtgtatcg atcatcaagg ttggcggata tattgacacc accacatcat cggaactgga
     1501 acacgccctg agcactcttc tcaaggcggg atcatacaat atcatcatcg acctgggcaa
     1561 tgtcgattac gtcagcagcg cgggctgggg catcttcatc agcgagatca aggggatccg
     1621 cgaaaagggc ggtgatctca aactggtgcg catgatccct gaagtctatg aggttttcga
     1681 gctgcttgag ttccactata tcctcaaggc ctgcgacagc attgacgagg ctataaagga
     1741 tttcgagcgc accctgggtc cagtcaccga ggggagcaag gtcgccgaag tggatatgga
     1801 agcggcgcac tcccggggcg gcggcgagcg ccgcgagagc gctccccggc cggagagcgg
     1861 cagggccgac aatctggaag agaagattcg caagatcatc ggacaggatc ccgagatcgg
     1921 caccatccag ctcatcagga ccctgaatac gccggagcat ggcaatatcc gtctcggctg
     1981 gtttgcgatg cgcaagctct tgaaaaaaag cggtctggac agcaaaaaag gtcgggccct
     2041 tctcagctcc ggacgctgac gcgcgcctgc gcggcaccaa ttcccgggtg gcggcacggc
     2101 ctctgcataa gccaagaccc cacccctttc ccacttgttc atctttcccc gatcttttcc
     2161 tatgttcata gaccatgcca agatacgtgt gcaagctggc tccggcgggg ccggctgtgt
     2221 cgctttccgc agagaaaagt atgtccccaa aggggggccc aacggcggcg acggcgggca
     2281 tggcggcgat gtcatcgcca tcgccgaccg gcatattcat accctgctcg atttcaaata
     2341 tcagacgctc ttcaaggccg agcgcggcca gcacggcatg ggctccaata tgaccggcaa
     2401 gtccgggcag cccctgctga tccgtatgcc catcggcacc accatccgga atgccgagag
     2461 cggggagctg attgccgacc tgaccaaaga gggggaggag gcggtcatcg cccgcggcgg
     2521 gcgcggcggc cgcggcaatt cccgtttcgt caccccgacc aaccgcaccc cgcgtgaatg
     2581 ggatccgggg gagttggggg aagaactcga actgactttg gagctcaagc tgattgcgga
     2641 tgtggggctg gttggcctgc ccaacgcagg caagtcgacc ctgctttcgc gcatctccgc
     2701 cgctcgcccg aaaattgctg actatccctt caccaccctc atccccaatc tgggcatcgt
     2761 ccgggtcagc gacgcccaga gcttcgttgt ggcggacatc cccggcctga tcgaaggcgc
     2821 ccatgagggc aaggggctgg gcatccagtt ccttcgccat attgaacgca ccaggatact
     2881 ggccatcctg ctcgattgca gctcggcgga gatggaagcg gattataaaa ccctcatcaa
     2941 cgaactgaaa tcctacagcc cggatctcct cagaaagaaa cgcctgctga tctacaccaa
     3001 aaaggatctt gtccagggca ggatcaagcc cctcaaggcc cgtctgcgcg gggacgcccc
     3061 cgagatcacc atctccgccg tgcgcgggga tggcctggag gagctgatcc gtctgatctg
     3121 ggataccttg cagcaggacg accaagcgga ttgacgcctt ccaacaggag gtgctatgga
     3181 atctgctacc cccgaattac tcagtttatg gaataccccg gggattggac cgcagcggct
     3241 gcgcagcctg atcgagcgct tcggcagcgg ccgggcggtc tatgccgcct ccacccggca
     3301 gttgtgcgag gttgagggca tagaccaggc gacggccgcc aagatcaagg ccaatagaga
     3361 tctggagggg gcaacccggg agctggaacg gacccgcaag gcaggtgtga ccgtcctttc
     3421 cctctgggat gcggcctatc cggagatgct ccgtcccctc tacgatttgc cggtgattct
     3481 gttcgtccag ggcgatctgg aggcgctctc ggccagggcg gtggcggtgg tcggtacgcg
     3541 cgttcccagc gagtacgggc agattgtcgc ccgcaagctg acgcgggagc tggtccagcg
     3601 tggcctggtc attgtcagcg gcctggcccg cggcatcgat accatcgccc accatgaggc
     3661 ggttacgcag ggcgggcgga ctgttgcagt gctcggttca ggtctcgatc agatctatcc
     3721 cggcgagaac cgccggttgg cgcggatgat cacggaacac ggcgccctga tctcggagta
     3781 tctcctcggc acccagccgg atgcccccca ttttccccgc cgcaaccgga tcatcgccgg
     3841 cctcgctgca gccaccctgg tggttgaggc cggcgagggc agcggggcgc tgatcaccgc
     3901 ggatgacgcc ctggagtaca accgtgaggt tatggcggtt ccgggcaata tcaccaatcc
     3961 gcgggcgtat gggtgcaacc acctcatcca gcagggggcc aaactggtgc agggggtgga
     4021 ggatatcctt gtggagatcg gcatggcgga ggagccgccg cagagccggc agctcgagct
     4081 gccgggggtg gggatttcgg ctgacgagga aaagctttta ggggttctca gccaggagcc
     4141 ccggcatgtg gatgagatcg ctcaggtgct gggcaggccg gtttccacag ttctggccca
     4201 gcttctcgag ctggagttga agagtctcgt caaccagctg ccggggaaga aatttgtgcg
     4261 cgcctgaggg caaacgaatg aggttctatg caaataatgt ttgactatct ttttaattat
     4321 ttgtaaattg acatctgccg ggagggatag acctaacagg caaggcagcg gtcttgaaaa
     4381 ccgccgggcg caagcccttg ggggttcgaa tccccctccc tccgcttcat gaggtcgatt
     4441 cccggcgaag acatattttc ggagaggtgc cggagtggct gaacggggcg gcctgctaag
     4501 ccgttgtagg gggtaacccc tacccagggt tcgaatccct gcctctccgc aacaaaaaat
     4561 ggcgaaattc gccatttttt tgtgagagtc aacgaaccag cagaaggatt ggttgtggcc
     4621 aagacaagag tgcgattcgc cccgagtccg acgggatttg ttcatatcgg cagtctgcgc
     4681 acagcgctct acaactatct ctacgcgcgc aatcagggcg gtgatttcat tctgcgcatc
     4741 gaggatacgg accgcgaacg gtttgtggag ggggcggtcg agaatctgat ttcagtgctc
     4801 gagtgggcgg ggttacgccc agacgagggc ccgcgctggg gcggagcctg tggcccctat
     4861 tttcagtccg agcgcacaga gacctaccgc cattatgccc aggagctgct caagaaaggg
     4921 tatgcctact actcctttga tacgccggcg gatatcgaag cgatgcgcgc gcgcgccaag
     4981 gcagcgggga atccctatgc caagtacaat ttcgccaacc ggatggagat gcgcaacagt
     5041 ttgagcctcc cgcccgcaga ggtcgaggag gcgatcgcgg acggcacccc ctacgccatc
     5101 cgcctgctcg ttccggaggg gcgcaagttc gaggttgagg atgaggttcg cggccatgtg
     5161 gtttttgaca gcaaggaggt ggatgaccag gtgctgctca agtcg
//
LOCUS       DAOPWU010000250         5180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16074_length_5180_cov_5.081366, whole genome shotgun sequence.
ACCESSION   DAOPWU010000250 DAOPWU010000000
VERSION     DAOPWU010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16074_length_5180_cov_5.081366"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PKM23_11465"
     CDS             complement(<1..1168)
                     /locus_tag="PKM23_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNY92130.1"
                     /translation="MEKGVAIEKAEGKLGVMLVGLGAVATTVVAGVELVRKGLAKPIG
                     SLTQMSTIRLGKRTDNRFPKIKDFVPLADLDDIVFAAWDIFADSAYDAALAAGVLTTE
                     DIEKIRPELQAIKPMKAVFNDYYVKRLHGTHVKSGKNKMDLAEQLKQDIAAFKKEHHL
                     SRVVVVWCASTEIYQEHDPVYDSVEALEKAMREDHIAIPPSMIYAYAAITSGIPFANG
                     APNITIDIPAMIELAKRHNVPVCGKDFKSGQTFMKTLIAPGLKAKQIGVSGWFSTNIL
                     GNRDGEVLDDPESFKSKEVSKLSVLDTIFQPEHNPDLYANVYHKVRINYYPPRGDNKE
                     SWDNIDIFGWLGYPMQIKVNFLCRDSILAAPIALDLALLLDLAQRSHMKGIQEWL"
     gene            complement(1202..1855)
                     /locus_tag="PKM23_11470"
     CDS             complement(1202..1855)
                     /locus_tag="PKM23_11470"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNY92131.1"
                     /translation="MKIELIPPAVKNGFKRMLQPLIGGLVRFRLNPNWFTTLSFFVVI
                     LSAVAFARGSLRWGAFLLLLGGLLDMVDGAVARASNRVTRFGALYDSVLDRYAEIAVF
                     FGIAYYFVHKIGEGSEHANIISMVVFLAMAGSLMVSYVRARAEGLDFDCRVGFLQRPE
                     RLILISLGALLNDSWLMVALMVIAFFANLTAVQRLVHIWRSEKSTRRNTLPPDIPHE"
     gene            complement(1861..2610)
                     /gene="rsmI"
                     /locus_tag="PKM23_11475"
     CDS             complement(1861..2610)
                     /gene="rsmI"
                     /locus_tag="PKM23_11475"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNY92132.1"
                     /translation="MSEAGYPAAQEPERSQEAEAGTLYLVSTPIGHLRDISLRALDIL
                     SQVDLIAAEDTRTSRVLLQHYRIQTPLTSYHDHNEERVAPALVGRLREGKSLALITDA
                     GTPGISDPGFYLVRAVLQAGLRVVAIPGATAFIPALVASGLPADRFVFEGFLPHKKGR
                     QTRLKLLQDEARTIIFYESPQRLERLLRELQVHLGDRPAAIAREISKKFEEIRRGTLS
                     ELIAGAEQMVKKGEFVLIVAGRPRNAKEASE"
     gene            complement(2737..4182)
                     /locus_tag="PKM23_11480"
     CDS             complement(2737..4182)
                     /locus_tag="PKM23_11480"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNY92133.1"
                     /translation="MAGPADSAHEATYHHLWRGPALTGRELFTLEGHRIEIVESGSAN
                     PDAGPDFLAALLRIDGELVRGDVEIHTRARDWYLHRHHKDPRYNRVILHAVVHPSPAG
                     FTARRQDGTAIPTLLLDAAVLPAEPVPTIVVSAPPSRCPLADLPAGRLLALLDQAGME
                     RLEMHAARFLEMREKAGWEEILHAGLFDALGYGKNQLPFRRLAALLPAAVLHAWTAGM
                     DEEEAVIGAEALLFGAAGLLPPPALPDGEEPAPGEDPETPGGHLARLREYWRRGGGEL
                     HLDPMPAEAWQFFRLRPCNFPTRRVAAAARLLTRFRRRGLLHELIAVVRAARFDPGGA
                     ILALNELAVVEVEGYWASHHDLEARRPAPRARRRVRLVGRGRSREIAVNVFLPGLLAF
                     ARESEDGRLSAAVRALYIASPRLPENDLTRRMRKKLFGGSGGGAAPGLQGAGAVRQQG
                     LIQLAQMRCRRNECEHCLALSPPEEKRQPRP"
     gene            complement(4198..4467)
                     /locus_tag="PKM23_11485"
     CDS             complement(4198..4467)
                     /locus_tag="PKM23_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92134.1"
                     /translation="MRGLLGAVADKAGAIKRSVKYHIDLIALRREVERLFSELGGRTY
                     ELLSRDPEAAIGRDPEILEWMKQLQEYDARLHARKNEHGEEAPGE"
     gene            complement(4527..>5180)
                     /locus_tag="PKM23_11490"
     CDS             complement(4527..>5180)
                     /locus_tag="PKM23_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNY92135.1"
                     /translation="KPELTAPGRIIGSSFSSEADPLSSYSVFASSNGSYPNAFILPDG
                     VHGLSLGTSMAAPHVSGAIALLMEKYPDLSASAARQVLIRTARSISGTAKANQWGYGK
                     LNAAALVSVNPDSLPEEEEGTLFLPYPNPFIISTTIPYPVPSADGNEKEPEILVYNIL
                     GERVPARLFRQSASFGRGAVIWYGRTIWEKRVAAGVYFVVFVYENRRVVRKICLLQG"
BASE COUNT          977 a         1570 c         1685 g          948 t
ORIGIN      
        1 agagccactc ctggatcccc ttcatatgcg agcgctgggc cagatcgagc aggagggcca
       61 gatcaagagc gatcggcgcg gccagaatgc tgtcgcggca gaggaaattg accttgatct
      121 gcatgggata gccgagccag ccgaagatat cgatgttgtc ccagctctcc ttgttgtcac
      181 cgcgcggggg gtaatagttg atgcgcacct tgtgatagac attggcatag agatccggat
      241 tgtgctcggg ctggaaaatg gtgtccagca ccgaaagctt ggaaacttcc ttgctcttga
      301 aggattcggg atcgtcgagc acctcgccgt cgcggttgcc gaggatgttg gtggagaacc
      361 agccggagac accgatctgc ttggccttga ggccgggggc gatcagggtc ttcatgaagg
      421 tctggccgga cttgaaatcc ttgccgcaga cgggcacgtt gtggcgcttg gccagttcga
      481 tcatggcggg gatgtcgatg gtgatgttgg gtgcgccgtt ggcgaagggt attccggaag
      541 tgatggccgc ataggcgtag atcatgctcg gggggatggc gatgtgatcc tcacgcatcg
      601 ccttctccag ggcctcgacc gaatcgtaga ccggatcgtg ctcctgatag atctcggtgc
      661 tggcgcacca gaccacgacc acgcgggaga gatggtgctc ctttttaaaa gcggcgatat
      721 cctgcttgag ctgctcggcc agatccatct tgttcttgcc gctcttgacg tgggtgccgt
      781 gcaggcgttt gacataataa tcattgaaaa ccgccttcat cggcttgatg gcttgcagct
      841 cgggacggat cttttcaata tcctcggtgg tgagtacgcc ggccgcaagc gctgcatcat
      901 aggcgctgtc cgcaaaaata tcccaggccg caaaaacgat atcatccaga tcggcgaggg
      961 ggacaaaatc cttgatcttg ggaaagcggt tgtcagtgcg tttgccgagg cggatggtgc
     1021 tcatctgggt gagcgacccg atcggtttgg ccagcccctt gcgcacgagc tcgacaccgg
     1081 ctaccaccgt agtcgccacg gcgcccaaac cgaccagcat gacgcccagt ttcccctccg
     1141 ctttctctat cgctactcct ttttccatga ccttgtcact ccttcatctt ttaaggtttg
     1201 cctattcatg aggaatatcg gggggcagag tgtttctcct ggtgctcttt tccgatctcc
     1261 agatatgaac aagcctttga actgccgtaa ggttggcaaa aaaggcaatc accatcaggg
     1321 ctaccatcag ccagctgtca ttgagtagag cccccaggct gatgaggatg agacgttccg
     1381 gacgctgcag aaatccgacc cggcagtcaa agtcgagccc ctcggcgcgc gcacggacat
     1441 agctgaccat cagggagccg gccatggcga gaaatactac catgctgatg atattggcgt
     1501 gttcactccc ctcgccgatc ttgtggacaa aataataggc gatcccgaaa aaaaccgcga
     1561 tctcggcata gcggtccagc accgaatcgt agagcgcccc gaaacgggtg acgcgattgg
     1621 aggcccgggc caccgccccg tcaaccatat ccagcaggcc gccgagcaga agcaaaaagg
     1681 cgccccagcg cagggagccg cgggcgaagg cgacggccga gagaatgacg acaaaaaagg
     1741 agagggtggt gaaccagttg ggattgagac ggaaacgtac cagcccgccg atgaggggct
     1801 gaagcatgcg cttgaaaccg ttcttgacag caggagggat cagttcgatt ttcatggtct
     1861 ttactcactc gcttccttgg cattgcgtgg acggccggcc acgatcagaa caaattcacc
     1921 ttttttgacc atctgctccg cgccggcgat cagttcggag agggtgccgc gccggatctc
     1981 ctcgaacttt ttgctgatct cccgggcgat ggccgccggg cggtcgccga ggtggacctg
     2041 gagttcgcgg aggaggcgtt cgagccgctg cggggactcg taaaagatga tggttcgcgc
     2101 ctcatcctgg agcagtttga gccgcgtctg cctgcccttc ttgtggggga gaaagccctc
     2161 aaagacgaag cggtcagcgg gcaggcccga agcgaccagg gccggaatga aagcggtggc
     2221 gccggggatg gcgacgaccc tgaggccggc ctgcagcacg gcgcgcacga ggtaaaagcc
     2281 gggatccgag atgcccggtg tgccggcatc ggtgatcagg gccaggctct tcccctcccg
     2341 cagccggccc accaatgcgg gggccacccg ctcctcgtta tggtcgtgat agctggtgag
     2401 gggcgtttga atccggtaat gctgcaggag aaccctgctg gtgcgtgtat cctcggcagc
     2461 gatgaggtcg acctggctga ggatgtcgag ggcgcgcagg ctgatgtcgc gcaaatggcc
     2521 gatcggcgtg ctgaccaggt agagtgtgcc ggcttccgcc tcctggctcc gctccggttc
     2581 ctgcgctgcg ggatatcctg cctcgctcat caccttgcct tccggccggc ttccggcccg
     2641 actgtcggaa aacgtttact tgaaaacctg ttaatttact tattaattgt ccaaaagtca
     2701 ataaaaagga taccttgacg ccgaagccgg ggcgaatcac ggccggggct gccgcttttc
     2761 ctccggtggg gagagagcca ggcagtgctc acactcattg cggcggcagc gcatctgcgc
     2821 cagctggatc aatccctgct gccggacggc ccccgccccc tgaagccccg gtgcagcccc
     2881 gccgccgctc ccgccgaaga gctttttgcg catccggcgg gtgaggtcgt tctccggcag
     2941 gcgcggactg gcgatataga gcgcccgcac ggccgccgag agccggccgt cctcgctctc
     3001 gcgcgcaaac gccagcagcc ccggcagaaa gacattgacg gcgatctcgc ggctgcgtcc
     3061 tcgccccacc agccgcaccc gccgtctggc ccggggcgcc ggccgccggg cctccagatc
     3121 gtgatggctg gcccagtagc cctcgacctc gaccacggcc agctcattga gggcgaggat
     3181 ggctccgccc ggatcaaagc gcgcagcacg aacgacggcg atcagctcat gcaaaagccc
     3241 ccgccggcgg aaacgggtga gcagcctcgc cgccgcggcc acccggcggg tgggaaaatt
     3301 acaggggcgc agccggaaaa actgccaggc ttcggccggc atcggatcga ggtggagctc
     3361 ccccccgccc cggcgccagt attcccgcag cctggccagg tggccgccgg gagtctccgg
     3421 atcctcaccg ggcgcgggct cttcaccatc gggaagcgcg ggcggcggca gcagtccggc
     3481 ggcgccaaag agcagtgcct cggcgccaat aacggcctct tcttcatcca tcccggccgt
     3541 ccaggcatga agcacggcgg ccggcagaag agcagccagc cgtcggaagg ggagctggtt
     3601 tttgccgtag ccgagggcgt cgaacagtcc agcatggagg atctcctccc accccgcctt
     3661 ctcccgcatc tccaggaagc gggccgcatg catttccagg cgctccatgc ccgcctgatc
     3721 gaggagggcg aggaggcggc ctgcggggag gtcggcgagg ggacagcggg aggggggagc
     3781 agacacaact atggtgggca caggctctgc ggggaggaca gcggcgtcga ggaggagggt
     3841 ggggatggcg gtcccgtcct ggcggcgggc ggtgaatccg gccggagagg ggtggacgac
     3901 ggcatggagg atgacgcggt tgtagcgggg atctttgtgg tggcggtgaa gataccagtc
     3961 gcgcgcgcgg gtatgaatct cgacatcgcc gcggacgagt tcgccgtcaa tgcggagcag
     4021 ggcggccaaa aagtcgggac cggcatcggg attggcgctg ccgctctcga ctatttcaat
     4081 cctgtgcccc tccagggtga agagctcgcg ccccgtcagt gcgggacccc gccagagatg
     4141 atgataggtt gcctcgtgcg ccgaatctgc cggtccggcc atggcgcctc ctgcaaatca
     4201 ctccccgggc gcctcctccc cgtgctcgtt cttcctggcg tgcaggcggg catcatactc
     4261 ctgcagctgt ttcatccatt ccaggatttc gggatcccgg ccgatcgcgg cttcgggatc
     4321 acgggagagc agctcgtagg tcctgccgcc cagttcggaa aagagccgct cgacctcccg
     4381 ccgcagggcg atgaggtcga tgtgatattt gacgctcctt tttatcgcgc cggccttgtc
     4441 cgcaaccgcg cccaggagtc ctcgcactcc acccctcctt ttcccggaaa ttgatgccat
     4501 catccgctac ctttcagccc cgccgcttac ccctgcagca gacaaatctt gcgcacaacg
     4561 cgccggttct catagacaaa gacaacaaaa tagacccctg cagccaccct tttttcccag
     4621 atcgtccggc cgtaccagat cacagcgccc cgcccgaagg aggcggactg ccggaacagc
     4681 cgggccggga cgcgctcgcc gagaatattg taaacgagga tctcgggctc cttttcgttc
     4741 ccgtccgcag agggcacagg ataggggatg gtcgtcgaga taataaaggg attgggataa
     4801 ggcaggaaga gggtgccctc ctcctcctcg ggtagcgagt cggggttcac gctcaccaag
     4861 gccgcggcgt tgagcttgcc atagccccac tggttggcct tggccgtgcc ggagatcgag
     4921 cgggcggtgc ggatcagcac ctgacgggcg gctgaggccg aaagatcggg atacttttcc
     4981 atgagcaggg cgatggcgcc gctcacatgc ggcgcggcca tgctggtgcc gagcgaaagc
     5041 ccgtgcaccc cgtcgggcag gatgaaggcg ttggggtagc ttccgttgga agaggcgaag
     5101 acgctgtagc tggagagggg atcggcctcg ctggagaagg agctgccgat gatccggcct
     5161 ggtgcggtca gctcgggctt
//
LOCUS       DAOPWU010000251         5175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16097_length_5175_cov_5.544336, whole genome shotgun sequence.
ACCESSION   DAOPWU010000251 DAOPWU010000000
VERSION     DAOPWU010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16097_length_5175_cov_5.544336"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..2322
                     /locus_tag="PKM23_11495"
     CDS             121..2322
                     /locus_tag="PKM23_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNY92136.1"
                     /translation="MSILIFGKQHTMIHFCHMKICPAGRLLHKGFLLATILFPGMNYA
                     AAEPGLLPRSQKVIHTTDRLLPEERTRIACIGNSVTYGIGVAGRDTCAYPAVLQCMLG
                     EQYTVGNFGRSGATLLKKGFRPYYRTEEFISALSFRPDIAIIHLGLNDTDPRAWPDFN
                     DEFIGDYLWLIDTLRSVGAKEFYIAKLSPIFNGHRRFKAGTRDWYWQIQERIEEVARL
                     SGATLIDFYTPLKNRPDLLPDNLHPDKRGAELLAKTVFSALSGDYGGLQLPEAWSDGM
                     VLQYDRKIRLRGTADRNETVQVCITGKSYSDVASATGEWEVSLDPLKAGGPYELVAMT
                     AKDTVIIRDILAGEVWLCSGQSNMEWPTGLSAGWEGMHESVSDGNLRIINFRRRQLGW
                     DVPWDSLTLAQINNLEYLKEGRWMAVSAETVEEFSAVAYYFGRKLREELRVPVGLIEM
                     TVGGSPAEAWIERKTLELHPQLVDLLYDYRKSEFREPWVRDVMTATLQLTDNPRQRHP
                     FEPAYLFEAGIARLAGFPIRGFIWYQGESNANFSELYQVMLPELVKNWRACWADSTLP
                     FYFAQLSSLNRPSWPEFRDVQRRLASRIPYSGMVVTTDVGHPTDVHPRDKKSVGDRFA
                     FLALKETYRKDSFRKSPEPFEAVRKGDKVIVSFRDAGKMRTSDGLPVREIEVSLSGEL
                     YEPASATIHGDTIIIASAGAVSVRYGWKPFSEGNLTGESGLPVPTFKLKIH"
     gene            complement(2673..3026)
                     /locus_tag="PKM23_11500"
     CDS             complement(2673..3026)
                     /locus_tag="PKM23_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNY92137.1"
                     /translation="MNGLYWKHLDALLESAEIIIDRPKGSPHPRYTFMIYPLDYGYLQ
                     GTASSDGAEIDVWLGSLPDRKCDAILCTADTVKRDVEVKLLVGCTEVEKATILDFHNS
                     SSLMSAILVRRTQAA"
     gene            complement(3310..4563)
                     /locus_tag="PKM23_11505"
     CDS             complement(3310..4563)
                     /locus_tag="PKM23_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNY92138.1"
                     /translation="MREKLYVLAGLLMLVIVPLKAQTIEGGRGGFTSTIDKEFTIAPG
                     GNLVMEIEGGDIRIVSGEKATVQVHETLTMKVFTRAEAEEIVKKLEASYSQSGSTLTI
                     RNEGTGHSLREHAYEITLPQKFNLDLRTAGGDLEISKVDGRLQLATSGGDIVLSTLAG
                     TIDARTSGGDLSLTNISGRLNAATSGGDVVAESIYAEGNLRTSGGDISAQNTTQRLDI
                     ATSGGDIVLKDIAGSFSAATSGGDVSLTQFSGSQGSLKTSGGDLVVNKSSGQLDLATS
                     GGDIDCQEIDKELNAATSGGDIEVRNLRAAATLRSMGGDISVAMTLQDFRIPHSLTAE
                     TTGGEIRVTLPAKLPATLEAEIRLRRRAGQDERNDIYSDFPLTKLPPDESSDRILRSK
                     GEINGGGDRIYLKTTGGDITITKGQ"
     gene            complement(4565..>5175)
                     /locus_tag="PKM23_11510"
     CDS             complement(4565..>5175)
                     /locus_tag="PKM23_11510"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNY92139.1"
                     /translation="LQALLTGFQPVVAAGHTVHPELLNVQRVRYDPASGQFEITYNTV
                     NDVRLQGTGNQPQVQLMLARAMVDSDNPALRLHAVKAARTIAAQQQSLDDELLQAIDY
                     LLSKEQNQGVRLMAIRVLRALPLTETIKRTLVQIVLHDENPALRMEAFDGLAAHAGGR
                     EIEPYLQTIAKDSSSSLRVKARTLRQTIDAGPVRSAELSQEN"
BASE COUNT         1054 a         1430 c         1506 g         1185 t
ORIGIN      
        1 aagatttgat catgagtgaa cttctggaca cctacaaagt ggccacggga ttgttcgacc
       61 tggtttttgc cagagacaga ttccctcgat ttctttgcca ccagtagcat caaatttcaa
      121 ttgtcaatac ttatcttcgg aaagcagcat acaatgattc atttttgcca catgaaaatt
      181 tgtcccgcag gccggctttt gcataaagga tttctccttg cgacgatcct tttcccgggc
      241 atgaactatg ccgcagcaga acccggattg ttgcccagat cgcaaaaggt catccataca
      301 acggaccgat tgctgcctga ggagagaacc aggattgcct gcattggcaa ctcggtaacc
      361 tacggaatcg gtgtggcggg cagggacaca tgcgcctatc ccgcagttct tcagtgcatg
      421 ctcggtgagc agtatacggt aggcaacttt ggccggagcg gcgcaacgct gttgaagaaa
      481 ggattcaggc cctactaccg gacggaagag ttcatctccg ctctatcatt cagacccgat
      541 atcgctatta ttcacctggg tctgaatgat accgatccac gggcctggcc ggactttaat
      601 gatgagttca tcggcgatta cctctggctg atagatacgc tcaggtcagt tggtgcaaag
      661 gagttctaca ttgcgaaatt atcaccgatt ttcaatggcc acagacggtt caaggccgga
      721 acccgggact ggtactggca gatacaggaa agaatagagg aggtcgcccg cctcagcggc
      781 gccaccctga tagattttta tacgccgctg aaaaaccgcc ctgacctgct gcctgataat
      841 ctgcacccgg acaaaagggg cgccgaactg cttgcaaaaa cggtcttttc ggcccttagc
      901 ggcgactatg gcggcctgca gctgccggaa gcatggagtg acggaatggt gctgcagtat
      961 gaccgcaaaa tcagactgcg aggcactgcg gacaggaatg aaaccgtgca ggtctgcatt
     1021 accggcaaaa gctactcgga tgtggcttcc gcaactggag aatgggaggt ctctcttgat
     1081 ccgttgaaag ctggaggtcc atatgaactt gttgccatga cggcaaagga taccgtaatc
     1141 atcagagata ttctggccgg cgaagtctgg ctctgctcgg gccagtccaa catggaatgg
     1201 cccacaggcc tttcagcagg atgggaaggg atgcatgaat cggtttctga cggaaatctc
     1261 aggataataa atttcaggcg caggcagctg gggtgggatg tgccgtggga ttcacttacg
     1321 cttgcacaga tcaataacct ggagtactta aaggaaggcc gctggatggc ggtctcagct
     1381 gagacggtcg aagaattttc cgctgtggcc tattacttcg gccggaagct cagggaagag
     1441 ctgcgagtac cggtcgggct cattgagatg acggtgggag gctccccggc cgaagcatgg
     1501 atagaaagga agactctcga gctgcatcca caactggttg acctgcttta tgactaccgc
     1561 aagagtgagt tcagggagcc ctgggttcgc gatgtcatga ccgcgaccct ccagctgacg
     1621 gataatccaa gacaacgcca cccttttgag cctgcttacc tctttgaggc gggcattgcg
     1681 aggcttgcag gttttccgat cagaggattc atctggtacc agggagaatc aaatgcaaat
     1741 ttctctgagc tctaccaggt tatgttacct gaactggtta aaaactggcg ggcttgctgg
     1801 gcggatagta ccctgccttt ttacttcgcc cagctttcaa gtctgaatcg accttcatgg
     1861 cccgagtttc gtgacgtaca gcgccggctt gcttcacgga taccttactc aggtatggtg
     1921 gtgacaacgg atgtgggtca cccgaccgat gtccatcccc gtgacaaaaa aagcgtggga
     1981 gatcgatttg ccttcctggc tctgaaggag acctaccgca aggattcatt caggaaaagc
     2041 cctgagccat ttgaggccgt cagaaagggt gacaaggtca tcgtatcttt cagagatgcc
     2101 ggtaaaatga ggacatccga cggcttgccc gtgagggaga tcgaagtttc cctttcgggt
     2161 gaattatatg agcccgcctc tgcaacaatt cacggtgaca cgataatcat cgcatcggcc
     2221 ggtgcagtat cggtaagata cggatggaag cccttctcgg agggaaatct gaccggggaa
     2281 tcggggcttc ctgttccaac gtttaaacta aaaatccact gatcagtggg gtgcattgat
     2341 gcaaggctga tacgattatg cagatcaacg aaatccttgt cctccaccac agccaccacg
     2401 acgtccgcta cacccacacc cagtcgctcg tctaggagct gcagcgcgag ttcatcgacc
     2461 aggccctcga tctcctcgaa tctgcctcgg gctgggacga atgctccgct cccaaattga
     2521 ccatcgaggt gaccgcttag gtagagaaat ggctcgaaac cgccgagcgc gcgatgatgc
     2581 gcttcatcga atcggcgttc taccacctcg agttcgaccc cgcgacaggc cggcgtgctc
     2641 tcttttgcgg cgaagcgccc cgggtgcttg cctcaggctg cttgtgtccg tcgcaccaga
     2701 atagcggaca tcaggctgct tgaattatga aaatcaagga tggttgcttt ttccacctct
     2761 gtgcatccca ccagcagctt gacctcaaca tcccttttca ccgtatcagc agtgcagagg
     2821 atggcgtcac atttccggtc cggcagtgaa cccaaccaga cgtcgatttc agcgccatca
     2881 ctgctggcgg tcccctggag atatccataa tcgagggggt agatcatgaa ggtgtagcgc
     2941 ggatggggcg aacccttcgg ccggtcgatg atgatctccg ctgactcgag gagggcatcg
     3001 aggtgtttcc agtacaatcc attcaagcgt tggttccgtc cttttttaag agcgcaaggg
     3061 tgctaatctc gtgaggttcc cggtctttac gcccctctct gacgattgtt ctcaacgccg
     3121 gagtgctcct cttgcctgaa tatcgctttt acttgatttg tggtcaagcc ttttttagtg
     3181 cagatgcccc ggcatgctca ataatagtgg cgatacaaac cttctccggt gcggggcggc
     3241 agccgccagg ccgatgcagg ccggacggct gccgcaatgt gcaatccctc gttgttgatg
     3301 gcgcttgctc tattgacctt tggtgatggt gatatcgcct ccggtcgtct tgagataaat
     3361 gcggtctccg ccgccgttga tttctccctt gcttcgcaga atccggtcgc tgctctcatc
     3421 gggcggcagc ttggtcagcg gaaagtcact gtagatatcg ttgcgctcat cctggccggc
     3481 gcggcgccgc aaccggatct cggcctccag ggttgccggc agtttggcgg gcagggtgac
     3541 gcggatctcg ccgccggtgg tttcggctgt caggctgtgg gggatgcgga aatcctgcag
     3601 ggtcatggcc accgagatgt cgccgcccat gctgcgcagc gtggcggcgg cgcggaggtt
     3661 gcggacctcg atgtcaccac cgctggtggc ggcgttcaac tccttgtcga tctcctgaca
     3721 atcgatatcc ccgcccgagg tggcgaggtc gagttgtccg gaggatttgt tcaccaccag
     3781 gtctcctccc gaggtcttta acgagccttg tgaaccgctg aattgggtca agctgacatc
     3841 cccgccagag gtggccgcgc tgaatgagcc ggcgatatcc ttaaggacga tatcgccgcc
     3901 cgaggtggct atgtcgagac gctgggtggt attttgcgcc gagatatcgc cgccggaggt
     3961 ccggaggttg ccctcggcgt aaatggattc agccacgaca tcgccgcccg aggtcgccgc
     4021 gttgagccgc ccggagatat ttgtaaggct cagatctcca ccggaggtcc tggcgtcgat
     4081 tgtcccggcc agggtcgaca gcactatatc gccgccgctg gtggccagct gcagccggcc
     4141 atccaccttg ctgatttcga ggtcgccgcc cgccgtccgg agatccagat tgaacttttg
     4201 cggcagagtg atctcatagg cgtgctcccg gaggctgtgg ccggtgccct cattgcggat
     4261 ggtcagggtc gatcccgact ggctgtagga ggcctcgagt tttttcacga tctcttccgc
     4321 ctcggcgcgg gtgaaaacct tcatggtgag ggtttcgtga acctggactg tcgccttttc
     4381 ccctgagacg atgcggatat cgccaccctc gatctccatg acgaggttac cgccgggggc
     4441 gatggtgaac tccttgtcga tggtgctggt aaagcctccc cgtccgccct cgatggtctg
     4501 cgccttcagc ggcacgatga cgagcatgag gagaccggca agtacataga gtttctctcg
     4561 catatcaatt ctcctgactg agttccgccg agcgcaccgg cccggcgtcg atggtttgcc
     4621 gcagcgttct ggctttgacg cgcagagagc tgctgctgtc tttggcaatg gtttgcaggt
     4681 agggttcgat ctcccggccg cccgcatgcg ctgcgaggcc gtcgaaggcc tccatgcgca
     4741 gggccggatt ttcatcgtga agcacgatct gcaccagcgt ccttttgatg gtttcggtga
     4801 gcggcaaggc gcgcagcacg cgaatcgcca tcaggcgcac cccctggttt tgctccttgc
     4861 tgagcaggta atcgatggcc tgaagcagtt cgtcgtcaag actttgctgc tgggcggcga
     4921 tcgttctggc cgccttgact gcgtgcaacc gcagcgccgg attgtcgctg tcgaccatcg
     4981 cccgggccag catcagctgg acttgcggct gatttcccgt gccttgtaac cggacgtcgt
     5041 tgacggtatt gtaggtgatc tcgaattgtc cgctggccgg atcgtagcgc acacgctgaa
     5101 cattcaacaa ttccggatgc acggtgtggc cggctgcgac tacgggttga aagccggtca
     5161 gcagcgcctg caggg
//
LOCUS       DAOPWU010000252         5174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16100_length_5174_cov_4.848994, whole genome shotgun sequence.
ACCESSION   DAOPWU010000252 DAOPWU010000000
VERSION     DAOPWU010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16100_length_5174_cov_4.848994"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKM23_11515"
     CDS             <1..249
                     /locus_tag="PKM23_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNY92140.1"
                     /translation="LKELAMSHQVLCITHLPQIAGMADHHLLVEKGGDEHQTRTTIRP
                     LSQEERVAQVAALIGGDVVTQAHLQSASELIAAAAGGS"
     gene            388..1419
                     /locus_tag="PKM23_11520"
     CDS             388..1419
                     /locus_tag="PKM23_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY92141.1"
                     /translation="MNSQSRSVWAVSIALVVIFVGLFLVCAQRRGTVAGGDDLSSNDE
                     EFRQELLQLLDLTEGEGGAEGEAAQTDEADLALADELAQGSDEESAENADAALTDDEL
                     LALLGAENETGDEAAALGQQEPPPAAGTQLESEPVTTTATMGLTPEMFNKMRTDISRL
                     ESALERSSATVDSLQSIIANRNARLQELESRKATPRIVIPDLSKTAKKPARAAASTAA
                     AGGSAFEAAYERARAKFEQRSYQAAIDEFSALLAQYPDHQLADNCQYWIGECYFGMKE
                     YQKAVVEFQKVFSYSETDKHDDAQLMVGLSYVRSNQAELAQKAFGTFLDNYAGSEYTS
                     VAKRYYRNI"
     gene            1434..4871
                     /locus_tag="PKM23_11525"
     CDS             1434..4871
                     /locus_tag="PKM23_11525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNY92142.1"
                     /translation="MSRFVHLHNHSHYSLLDGACRIEDLVERAEKQGMPAIALTDHGC
                     MHGVISFYKKATKAKVKPLIGSEVYVAPGSRLEKQPDPGTGGTAFHLVLLCKDMTGYR
                     NLMKLVSAGYLEGFYYKPRIDRALLREHHEGLIALSACLKGEVSQRLLRENEERSRAA
                     IAEYHDIFGEDFYLEVQNHGIREEETARSAIFGFSQEMGIKVVATNDIHYLEKGHAEA
                     HDILLCLQTGKDFEDATRMRYNTRELYFKSEAEMAEAFPDHPEALLNTLEVAEKCDLH
                     LDSRTFHLPVFQLPPEHAGKSLDDYLAEVARAGLRERYPAVTPELEKRLEYELEMIKR
                     MGYAGYFLITADFISYARSQDIPVGPGRGSAAGSLVAYVLRITNLDPIRYDLIFERFL
                     NPERISMPDIDIDFCYERREEVIRYVKEKYGYSNVCQIITFGTMAARAVIRDVGRVLK
                     INYSEVDRIAKLVPEQLKIKLKEALQNVAELRQISEKDETGRRLIEHALVLEGLARHA
                     STHAAGVVITPEELTNYVPLYKTKEGDITTQYDMKSLEEVGLLKMDFLGLRTLTVIQK
                     AIKALAARGIRIDIDAIPLDDPKVYEVFARGETIGLFQFESSGMQEYLRKLKPTCLED
                     LIAMNALYRPGPMDYINDFIRGKRDAASISYLHPLLRPLLEETYGIAVYQEQVIKMAT
                     EVGGFSLGNADLLRRAMGKKDLEIMARERVHFTDGANQRGVPNKVADEIYDQMAKFAG
                     YGFNKSHAACYSLVAYQTGFLKVHYPAEFMAANLSSEMSSTARVVVLLEECRRMGLEV
                     LAPDVNESFADFIVSEGRIRFGLGAVKNVGRGAIEAIVAGREKKGRFATLVDLVCAID
                     LQAVNKKVLESLIQAGAMDSLAGTRAQKFEGLEEAINYAQAINRSAALGQFSFFDGGE
                     EKGEESESLRPPLPLVADWPEGDALNREKAMLGFYLTGHPLDRFREDIRSLSTVPLDQ
                     AGECKDNSKVRLCGLITEVKTLLDRKQRPMAFFKVEDFTGSIEGLTFAEAYDKNRAAI
                     QVDQIIMALGRISTREGDAPKLVVEEVIPLEEARKRFTRSLFLSLDPGSADEELLAGL
                     KQTLSEFTGSVPLFLRIKGSDDGDYFLRSRSITVTPSLALLDRLRAQVGRENVWVGA"
     gene            4873..>5174
                     /locus_tag="PKM23_11530"
     CDS             4873..>5174
                     /locus_tag="PKM23_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92143.1"
                     /translation="MSPAKKASDAEICRRLAGLVRAETLGLEEADWRAFCARMERTLC
                     APAPAGGGLLIHIDGAARGNPGPAAIGVILAESGGRTVREHGARIGETTNNVAEYRA"
BASE COUNT         1037 a         1680 c         1569 g          888 t
ORIGIN      
        1 ctcaaggaac tggccatgag ccatcaggtg ctctgcatca cccatctgcc gcagatcgcc
       61 ggcatggccg atcatcattt actggtcgaa aagggggggg acgaacatca gacgcgcacg
      121 acgattcgtc ccctttcgca ggaagaacgc gtggcccagg tagccgccct catcggcggc
      181 gatgtggtca cccaggccca tctgcagagc gccagcgagc ttatcgcggc cgctgccggc
      241 gggtcctgac cacccgcagg ggcgcggtgg tgaagcagca cgggttgggg tgtgattcgg
      301 agcaactaca gaacgcagcg ctattgagcc gagagatcaa gcaaccggtc taccttaact
      361 gtcaggcatc ctagcaacga ggttcacatg aacagtcaat cacgttccgt ctgggcggtt
      421 tcgatcgccc tggtcgtcat cttcgtcggg cttttcctgg tctgtgcgca gcgccggggt
      481 actgtcgcgg ggggtgatga cctttccagc aacgacgagg agtttcgtca ggaactgctg
      541 cagcttctcg acctcaccga gggtgagggt ggggcagagg gagaggctgc gcaaaccgat
      601 gaagcagatc tcgccctggc cgacgagctt gcgcagggca gcgatgaaga gtcggccgag
      661 aacgctgacg ccgccctcac tgatgacgag ctgctcgccc ttctcggtgc ggagaatgag
      721 accggcgacg aagccgccgc gctcggacag caggaacccc cgcccgccgc gggaacgcag
      781 ctggagagcg agccggtgac cacaacggcc accatggggc tgacgccgga aatgtttaac
      841 aagatgcgca ccgacatcag ccgcctcgag agcgcgctcg agcgcagcag cgccacggtg
      901 gacagcctgc agagcatcat cgccaatcgc aatgccaggc tgcaggaact ggagagccgc
      961 aaggcgaccc cccgcatcgt catcccggat ctgtcgaaaa ccgccaaaaa gcccgcccgc
     1021 gccgccgctt cgactgctgc cgcgggaggc agcgccttcg aggccgccta cgagcgcgcg
     1081 cgcgccaaat tcgagcagcg cagctaccag gctgcgatcg atgaattctc cgcactgctc
     1141 gcccagtatc ccgaccacca gctcgccgac aactgccagt actggatcgg cgaatgttac
     1201 ttcggtatga aagagtacca gaaggcagtg gtcgaatttc agaaggtctt ctcctactcc
     1261 gaaaccgaca agcatgacga cgcccagctc atggtcgggc tctcctatgt ccgctcgaac
     1321 caggcggagc tggcacaaaa ggccttcggc actttcctcg acaactatgc tgggagcgaa
     1381 tatacttcgg tcgccaagcg ctattatcgc aacatttaag caagagttac cgcgtgagcc
     1441 ggtttgtcca tctccataac cactcccact acagccttct cgacggggcc tgccgcatcg
     1501 aggatctcgt cgagagggcg gaaaaacagg gcatgccggc gatcgctctc accgaccatg
     1561 gctgcatgca tggggtcatc agcttttaca aaaaggccac caaggcaaag gtcaagcccc
     1621 tgatcggctc cgaggtctat gtcgccccgg gcagccgtct ggagaagcag cccgatcccg
     1681 gcacgggagg caccgctttc catctcgtcc tgctctgcaa ggacatgacc ggttaccgca
     1741 acctcatgaa gctggtctcg gccggctatc tggaaggttt ctactacaag ccccgcatcg
     1801 accgcgccct gctgcgcgaa catcatgaag gattgatcgc cctcagcgcc tgcctcaagg
     1861 gagaggtcag ccagcggctg ctgcgcgaga acgaggagcg cagccgggcc gccatcgccg
     1921 aataccacga catcttcggc gaagatttct acctcgaagt ccagaatcac ggcatccgcg
     1981 aagaagagac cgcccgcagc gccatctttg gcttttctca agagatgggc atcaaggtgg
     2041 tggcgaccaa cgatatccac tatcttgaaa aggggcacgc cgaggcccat gacatcctcc
     2101 tttgtctgca gacgggcaag gatttcgagg atgccacccg catgcgctac aacacccggg
     2161 agctctattt caagagcgag gcggagatgg ccgaggcttt cccggaccat cccgaggccc
     2221 tgctcaacac cctggaggtg gcggaaaagt gcgacctcca ccttgattcc cggacctttc
     2281 acctccccgt tttccagctg ccgcccgagc acgccggcaa gagtctcgac gactatctcg
     2341 ccgaggtggc ccgggccggc ctgcgcgagc gctatccggc ggtgacgccg gagctggaga
     2401 agcggctgga gtatgaactc gagatgatca agcgcatggg ctatgcgggt tattttctca
     2461 tcaccgccga tttcatcagc tatgcccgca gccaggacat cccggtgggg cccggccgcg
     2521 gctcagccgc cggcagcctg gtcgcctacg tgctcaggat caccaatctc gatccgatcc
     2581 gctacgacct catcttcgaa cgctttctca atcccgaacg catctcgatg cccgacatcg
     2641 acatcgactt ttgctacgag cgccgcgaag aggtgatccg ctacgtcaag gaaaagtacg
     2701 gctacagcaa cgtctgccag atcattacct tcgggaccat ggcggcgcgg gccgtcatcc
     2761 gcgatgtcgg gagggtcctc aagatcaact attccgaggt cgaccgcatc gccaagctgg
     2821 tgccggaaca gctcaagatc aagctcaagg aggccctgca gaatgtcgcc gagctgcggc
     2881 agatctccga gaaggatgag accggccgac gcctcatcga gcatgccctc gtcctcgagg
     2941 gactggcgcg gcacgcctcc acccacgccg ccggcgtggt catcaccccg gaagagctga
     3001 ccaactacgt gccgctctac aagaccaagg agggcgacat caccacccag tacgacatga
     3061 agtcgctgga ggaggtgggt ctgctcaaga tggattttct cggcctgcgc actttgacgg
     3121 tgatccagaa ggcgatcaag gccctggccg cccggggcat ccggatcgac atcgacgcca
     3181 tcccgctcga tgatcccaag gtctacgaag tcttcgcccg cggcgagacc atcggcctct
     3241 tccagtttga aagttcgggc atgcaggaat atctgcgcaa gctcaagccc acctgtctgg
     3301 aagacctcat cgccatgaat gccctctacc gtccggggcc catggattac atcaacgatt
     3361 tcatccgcgg caagcgcgat gctgcctcga tcagctatct ccatcccctg ctcaggcccc
     3421 tgctcgaaga gacctatggc attgcggtct atcaggagca ggtgatcaag atggcgacgg
     3481 aggtgggggg tttttcgctc ggcaacgccg acctgctccg ccgcgccatg ggcaagaaag
     3541 atctggaaat catggccagg gagcgggttc atttcaccga cggggccaac cagcgcggcg
     3601 tgccgaacaa ggtggccgat gaaatctacg accagatggc caagttcgcc ggctacggct
     3661 tcaacaagtc ccatgccgcc tgctactccc tggtcgccta tcagacaggc tttctcaaag
     3721 tccactaccc ggccgaattc atggcggcga acctgagcag tgaaatgagc agcaccgcgc
     3781 gggtggtggt cctgctcgag gaatgccgcc gcatgggtct ggaggtgctg gccccggatg
     3841 tcaacgaaag tttcgccgat ttcatcgtca gcgaagggcg catccgtttc ggcctcgggg
     3901 cggtcaagaa tgtcggccgc ggggccattg aggccatcgt cgccggccgt gagaagaagg
     3961 ggcgcttcgc gaccctggtc gatctggtct gcgccattga cctgcaggcg gtgaacaaga
     4021 aggtcctgga aagtctcatc caggccgggg cgatggattc cctggccgga acccgggcgc
     4081 agaaattcga gggcctcgag gaggccatca attacgccca ggcgatcaac cgcagcgcgg
     4141 ccctcggcca gttctccttt tttgacggcg gcgaggaaaa gggcgaggag agtgagagct
     4201 tgcggccccc cctccccctg gtggccgact ggcccgaggg cgatgccctc aaccgcgaaa
     4261 aggcgatgct cggtttttat ctcaccggcc accccctcga ccgctttcgc gaggatatcc
     4321 gttcgctttc caccgtcccc ctcgatcagg ccggggagtg caaggacaac agcaaggtcc
     4381 gcctctgcgg gctgatcacc gaagtcaaga ccctccttga ccgcaagcag cggccgatgg
     4441 cctttttcaa ggtcgaggat ttcaccggca gcatcgaggg acttaccttc gccgaggcct
     4501 acgacaagaa tcgcgccgcc atccaggtcg atcagatcat catggccctc ggccggatca
     4561 gcacccgcga gggcgacgcc cccaagctcg tcgtcgagga ggtcatcccc ctcgaggagg
     4621 cgcgcaagcg cttcacccgc agcctctttc tctctctcga ccccggttcg gccgatgagg
     4681 agctgctggc cgggctcaag cagaccctca gcgaattcac cggaagcgtg cccctctttc
     4741 tgcgcatcaa ggggtcggat gacggcgact atttcctgcg ctcccgctcg atcacggtca
     4801 caccgagtct ggcgctgctc gatcgcctgc gcgcgcaggt ggggcgggag aatgtctggg
     4861 tgggggcctg agatgagccc ggcgaaaaag gccagcgatg cggagatctg ccggcgcctg
     4921 gccggactgg tgcgggccga gactctgggc cttgaggagg cggattggcg tgctttttgc
     4981 gcgcgcatgg agcgcaccct ttgcgctccg gctccggccg gcggcgggct gctcatccac
     5041 atcgacggcg ccgcccgcgg caaccccggc ccggcggcca tcggggtgat ccttgctgaa
     5101 tccggcggcc gcaccgtgcg cgaacacggc gcccgtatcg gcgaaaccac caacaacgtc
     5161 gccgagtacc gcgc
//
LOCUS       DAOPWU010000253         5167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16134_length_5167_cov_3.843505, whole genome shotgun sequence.
ACCESSION   DAOPWU010000253 DAOPWU010000000
VERSION     DAOPWU010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16134_length_5167_cov_3.843505"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKM23_11535"
     CDS             <1..239
                     /locus_tag="PKM23_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92144.1"
                     /translation="QAYYEPLSMIPAPAATEAAAGEFLLRSGTGISIDPAFESSGEVA
                     DMFNAFLKKHYGKIRVSEQQNQGDANVIWARHDT"
     gene            338..832
                     /locus_tag="PKM23_11540"
     CDS             338..832
                     /locus_tag="PKM23_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNY92145.1"
                     /translation="MQIIFPSQKCDRGTVAVPCVKIADSPRYRWRGMHLNVSRHFFPK
                     EFIFKMLDAMAVHKLNTFHWHLTDDQGWRIEIEKYPELATVAAWRDETLIGYGSETPW
                     VSDGTRYGGCCTQQDVREVVEYARKLHINVLPEIEMPGHAVAALQAFPQLFCNGGEPP
                     PFNR"
     gene            complement(822..1031)
                     /locus_tag="PKM23_11545"
     CDS             complement(822..1031)
                     /locus_tag="PKM23_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92146.1"
                     /translation="MRLEHLQPFVTALQDEFYTDDEMLAESVQRHSSFNIIHRETMFK
                     VDVFVPRPRPFLQSQLASVPLPFTC"
     gene            complement(1165..1386)
                     /locus_tag="PKM23_11550"
     CDS             complement(1165..1386)
                     /locus_tag="PKM23_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92147.1"
                     /translation="MEALQIQLWRQASPTRKMKMPVQLNKSAQILALAGLRMRTPQAS
                     EAEIHCRLAGLSLGEESARKVYGENNHAK"
     gene            complement(1579..2772)
                     /gene="nhaA"
                     /locus_tag="PKM23_11555"
     CDS             complement(1579..2772)
                     /gene="nhaA"
                     /locus_tag="PKM23_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNY92148.1"
                     /translation="MTSATDSFIRRFLKQEAAGGILLVLFAVLAIAAANSPLYALYRR
                     LLEMPLEVQVGSLEIAKPLLLWVNDGLMAIFFLLVGLELKREFLQGELSSRKKVVLPG
                     LGALGGMLIPAAIYIIFNARDPIALQGWAIPAATDIAFALGVLALLGSRVPVGLKIFL
                     TSLAIFDDIGAIIIIAFFYTSKISFAALIVTGACLLVLFVLNRRGIAQKSIYLTIGVI
                     MWIALLKSGVHATLAGILLAIFIPIKSTAPSESSPLQSLEHDLHGAVAFIIMPLFAFC
                     NSGIRFSGMGWEQVAHPIPLGIALGLFFGKQLGVFVFCWLGIKWGVAELPTRATWLSF
                     YGTAILCGIGFTMSLFIGSLAFEETGANLLIDERLGILAGSLASGLYGYWILRLATKP
                     AAPSS"
     gene            complement(2871..>3173)
                     /locus_tag="PKM23_11560"
     CDS             complement(2871..>3173)
                     /locus_tag="PKM23_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNY92149.1"
                     /translation="GLFIVPVDHPFVALSTYLLLKQACGQNPGAIIKPVYNGRPGHPV
                     VVPIALSEAILAAEPGASLRDLLRGAGTELIRVEAGDAGILKNINEPGDRLIITDT"
     assembly_gap    3174..3273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3355..4806)
                     /locus_tag="PKM23_11565"
     CDS             complement(3355..4806)
                     /locus_tag="PKM23_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNY92150.1"
                     /translation="MSQIIKELHPEIIRKTAARCKRLGITIPTFRQLRQPELPASFME
                     RLQPVGLWDINPLNLYRITWKNDIRTGLFGGVNYLEIPPALSGVKARIIGLVGQYFPT
                     GAHKVGAAFGCLVPRLVSGEFDPEVHKAVWPSTGNYCRGGAFDCALLGCTAVAILPEE
                     MSRERFDWLREIGAEVIATPGCESNVKEIYDKCHELRRDPLNVILNQFDEFGNPIFHY
                     AVTGPAFDEVLADLNGALRPAAFISATGSAGTIATGDYLKKQHPGLKVVASEALQCPT
                     LLVNGFGGHRIEGIGDKHVPWVHNVRNTDAVAAIDDEDCMRLFRLFNEPAGQEYLAET
                     GLDPNLIQSLPLLGISSIANLLAAIKSAKYWELGGDEVILTIFTDSAEMYRSRLTELA
                     AACGAYRRDDALRDHAGPLAHQSVDYFRELTYTDRKAIHNLKYYTWVEQQGKSSEELN
                     AQWDPEYWRALFEDEVDYFDELIVEFNGEIANS"
     gene            complement(4815..>5167)
                     /locus_tag="PKM23_11570"
     CDS             complement(4815..>5167)
                     /locus_tag="PKM23_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HNY92151.1"
                     /translation="PYQVMQERTALLKLGDREGLKALEQTCLANNARFKNWECDEAKM
                     KLTKGGSALYMHCLPADISGVSCERGEVAREVFEKYRLETYREAGCKPFVIAAMIAMA
                     RIPDLPTQLARMNA"
BASE COUNT         1105 a         1407 c         1525 g         1030 t
ORIGIN      
        1 atcaggcata ttatgaaccc ctatccatga tcccggctcc tgctgcaaca gaagcggcgg
       61 caggggaatt cctgctcagg tccggaacag ggatttcaat agaccctgcc ttcgaatcct
      121 ccggcgaggt tgccgatatg ttcaacgctt ttctaaaaaa gcattatggc aaaatcaggg
      181 tttcagaaca gcagaatcaa ggagacgcca acgtaatctg ggccaggcat gacacctgat
      241 tgaaaaccct gaggcttaca ggcttgcagt aaccggggag ggtataatca ttgacgcccg
      301 cggcgatgcc ggacttctct atgctttcca gaccctgatg cagatcatct ttccatcgca
      361 aaagtgtgac agggggactg tggctgtccc gtgtgtgaaa attgcggatt caccccggta
      421 caggtggcgt ggcatgcacc tgaatgtctc gcgacatttc ttcccgaagg agttcatctt
      481 taagatgctt gacgcgatgg ctgtgcataa gctcaacacc ttccactggc atctgaccga
      541 tgaccaggga tggaggattg agattgagaa gtatcccgag ctggccactg tggctgcatg
      601 gagggatgag acgctgatcg gctacggcag cgagacgcca tgggtatctg acggcacaag
      661 atacggaggc tgttgcactc agcaggatgt gcgtgaggtg gtggaatatg cccgcaagct
      721 gcacatcaac gtattgccgg agatagagat gccaggtcat gccgtggcag cattgcaggc
      781 ttttccgcag ctcttctgca acggaggtga acctcctccc ttcaacaggt gaagggtagt
      841 ggaacgctgg ccagctgcga ttgtaaaaac gggcgcgggc gcggcacaaa aacatccacc
      901 ttgaacatcg tttcacgatg gatgatgttg aaactggaat gccgttgaac tgattccgca
      961 agcatctcat cgtctgtgta gaattcatct tgcaatgccg tgacaaacgg ttgcagatgc
     1021 tccagccgca tgtcggctac gatatctcaa tcttgcgtgg ttcgtaccat accgtaaagc
     1081 gctctggcta gcgaccctcc gataagatag gaaattccaa gcgattcaag aaccctggtt
     1141 acattgaacg tgacttcaat cggttcattt tgcatggttg ttttccccat acactttgcg
     1201 tgcggattct tctcccaatg ataggccagc caacctgcaa tggatttcag cttcactggc
     1261 ctggggagtt cgcatgcgca gacccgctaa cgcgagtatt tgtgccgatt tgttgagctg
     1321 caccggcatc ttcattttgc gagtcgggct ggcctgccgc cacagttgaa tttgcagagc
     1381 ttccattttt ggacaagttc agggaataag gcgctcattg cttgcagccc ttcataaagg
     1441 ccagaacagt ggtgatacga ttgtgctcct tgacgcgttt tcagtaattt tgtagcaaac
     1501 gccacacccg tcattcgtca gcttgacacc gttcgaagta tagcgtgtgt gctcctgcgc
     1561 ccgcttgggg tgaaagtctc agcttgatgg tgcagcgggt ttggtcgcca gacgcagaat
     1621 ccagtagcca tacaggccgg aggccaggga acccgccaaa atgcccaggc gctcgtcgat
     1681 gagcaggttt gcgccggttt cctcaaatgc aagggagccg atgaacaagc tcatggtaaa
     1741 accgattccg cacagaatgg ctgtgccata aaaggagagc caggttgccc gggttggcag
     1801 ctcggccact ccccacttga tccccagcca gcaaaagaca aagacgccca attgtttgcc
     1861 gaaaaacaaa ccgagggcaa tcccgagcgg aatgggatga gccacctgtt cccagcccat
     1921 gccggagaaa cggatgccgg aattgcagaa agcgaataag ggcataatga taaaggccac
     1981 tgcgccatgc agatcgtgct cgaggctttg cagcggagat gattcagagg gcgcggtgga
     2041 tttaatgggg atgaaaatgg ccagcaggat gcccgccagc gtggcgtgca cgcccgactt
     2101 gagcagcgcg atccacataa tgacaccgat ggtcaagtaa atgcttttct gtgcaatgcc
     2161 gcgacggttg aggacaaaaa gaacaagcag gcacgcgccg gtgactatga gggcggcaaa
     2221 ggagatcttg ctggtataaa agaaagcgat gatgataatg gcgccgatat catcaaagat
     2281 ggccagggag gtgaggaaaa tcttcagtcc gactggcacc cgggagccca gcagggcaag
     2341 cacgcccagg gcaaaggcga tgtcggtggc agccgggatg gcccagccct gcagggcaat
     2401 gggatcccgg gcattgaaaa taatatagat ggccgcaggg atgagcatgc cgccgagagc
     2461 gcccaaaccc ggcaggacga cctttttcct gcttgacaac tcgccctgca aaaattcgcg
     2521 tttcagctcc aggcccacaa gaaggaagaa gatggccatg aggccgtcgt tgacccaaag
     2581 cagcagtggc ttggcgattt ccagacttcc cacctgcacc tcaagcggca tctccagcag
     2641 ccggcggtag agggcataga gcggcgaatt ggcggcagcg atggccagca cggcaaagag
     2701 caccaacaag atgccgccgg ccgcttcctg cttgagaaac cttcgtataa aggaatcagt
     2761 tgcgcttgtc ataatggctc ccgttgtctg catttcgagg cgtgccaaag gcttttcctc
     2821 cggcggatat ttaccccccg gcaaaaaaaa atcactcagt accagtcctg tcaggtatca
     2881 gttattatta accggtcacc cggctcattg atgtttttca ggatgcctgc atccccggcc
     2941 tcgacccgga ttagctctgt gccggcacca cgcaaaagat cgcgcaggga ggcgccgggc
     3001 tctgctgcca aaatcgcctc agagagagcg atgggaacaa cgacgggatg gcccggccgg
     3061 ccattgtata caggcttgat gatcgcaccg ggattttgcc cacaggcctg cttgagcagg
     3121 aggtaggtcg aaagggcgac gaaaggatgg tcgaccggta cgatgaatag cccnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntccaggc gcagttttgg ggtgccgatg
     3301 cggcggccgc tcccggccgc caggatcact gcgccgatgg cgccggggct ttcctcagga
     3361 attggcgatc tctccattga actccacaat cagctcgtcg aaataatcca cctcatcctc
     3421 gaacagcgcc cgccagtact ccggatccca ctgggcgttg agctcctcgc tgctcttgcc
     3481 ctgctgctcc acccaggtat agtatttgag attatggatc gccttgcggt cagtataggt
     3541 caactccctg aaatagtcga ccgactgatg agccagggga ccggcgtggt cgcgcagggc
     3601 gtcatcgcgt cggtaggccc cgcacgcggc ggccagttcg gtcagcctcg agcggtacat
     3661 ttcggcggaa tcggtaaaaa tggtcaggat gacttcatcc ccgccgagct cccagtattt
     3721 ggcgctcttg atggcggcaa ggagattggc gatggaggag atgccgagca ggggcaggct
     3781 ctggatgagg ttggggtcaa ggccggtttc ggcgaggtat tcctgtcccg ccggctcgtt
     3841 gaagaggcgg aacaggcgca tgcagtcctc gtcgtcgatg gcggcgacgg cgtcggtgtt
     3901 gcggacgttg tgcacccagg gcacatgctt gtcgccgatt ccctcgatgc ggtggccgcc
     3961 gaagccgttc acgagcaggg tggggcactg cagggcctcg ctcgccacca ccttgagccc
     4021 ggggtgctgc ttcttgagat aatccccggt cgcgatggtg ccggccgatc ccgtcgccga
     4081 aataaaggcg gccgggcgga gggctccatt caggtccgcg agcacctcgt caaaagccgg
     4141 tccggtgacg gcgtaatgga agatcggatt gccaaattca tcgaactggt taaggatgac
     4201 gttgagggga tcgcggcgca gttcgtggca cttgtcatag atctccttga cgttcgactc
     4261 gcagcccggg gtggcaatca cttcggcgcc gatctcgcgc agccagtcaa agcgctcccg
     4321 cgacatctcc tcggggagga tggccaccgc ggtgcagccg agcagggcgc agtcaaaagc
     4381 cccgccgcgg cagtaattgc cggtcgaggg ccagaccgcc ttgtgtactt ccgggtcgaa
     4441 ttcgccgctg accaggcgcg gcaccagaca gccaaaggcc gcgccgacct tgtgcgcccc
     4501 ggtcggaaaa tactggccca ccagtccgat gatgcgcgcc ttcaccccgc tcagggccgg
     4561 gggaatctcc agataattga ctccgccgaa gagtccggtg cggatatcgt tcttccaggt
     4621 gatgcggtaa aggttgagag gattgatatc ccagagacca accggctgca gccgctccat
     4681 aaagctggct ggcagctccg gctggcggag ctgccgaaag gtggggatgg tgatgccgag
     4741 ccgcttgcag cgcgcggcgg ttttacggat gatctcgggg tgcagctctt tgatgatttg
     4801 cgacatgaca gctcctaggc gttcattcgg gccagttggg tgggcaggtc agggatcctg
     4861 gccatggcga tcatcgcggc gatgacgaag ggcttacagc ccgcctcccg gtaggtctcg
     4921 agccggtact tctcaaagac ctcgcgggcg acttcgccgc gctcgcagct caccccggag
     4981 atgtccgccg gcaggcagtg catatagagg gcgctgccgc ccttcgttag cttcattttc
     5041 gcctcgtcac actcccagtt tttaaagcgc gcgttattgg ccagacaggt ctgttccagc
     5101 gccttgagcc cctcgcggtc gcctagcttg agcagggcgg tgcgttcctg catcacctgg
     5161 taggggg
//
LOCUS       DAOPWU010000254         5166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16137_length_5166_cov_4.953042, whole genome shotgun sequence.
ACCESSION   DAOPWU010000254 DAOPWU010000000
VERSION     DAOPWU010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16137_length_5166_cov_4.953042"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1325)
                     /locus_tag="PKM23_11575"
     CDS             complement(207..1325)
                     /locus_tag="PKM23_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92152.1"
                     /translation="MHNGRGSAGFFLAMVLFLLAGPLAAQQLCDYGAEGLPAAAHLYD
                     PGVFIGRPSGGPWIDGESVARDDSDGEGIQFSFNGREVTCFLSRDGVMAGWIDFDGGE
                     QGGVDFSQAADNIIPPQPVRAGYNSFSFAWPEGYRPGQEVWARFRFSSSRQPNAVQSP
                     AGLSDAAGEVQDYRFDLTPIALGSFEAHPENAAVRLEWQTQAENDNLGFHLFRACSEE
                     GPYSQITRNMVRGAGSSTFRRSYEYSDSTVEPGKSYYYKLADVDYRGRMTMHGPVRVS
                     ATAPVDYVLEQIYPNPFNPETRINFRLKEAGKVLLSIFDLRGRQVRRLMAAALPGGTH
                     TVSWNGKDDNGLNLSSDTYIYKLQVDDYEISRRIKFLR"
     gene            complement(1577..2791)
                     /locus_tag="PKM23_11580"
     CDS             complement(1577..2791)
                     /locus_tag="PKM23_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNY92153.1"
                     /translation="MQAVMMVAGKSTRTYPLTLTRPKPLLPVANRPLIYHSLDQMAGV
                     FDEVILIVGYRQEMIRALLGDEYRGIHLIYQEQKQQLGTGHAILQAKPHIRGRFVACN
                     GDDLFAREDFLKLLEHPYAALVKPVADPSLYGVAVVDAHYRLIQMVEKPKTFLGNLAN
                     IGCYIFEPDIFADLEKVELSERGEIEIIGAIMATVQRSTFSIVPITGFWLPTGFAWDL
                     LKHQEFIMAGMSSSRIEGRIEAGAVLNGAVQVGAGTVIRSGSYIEGPVAIGRNCDIGP
                     NCYVRRFTAIGDNCRIGHAVEIKNSIIMPGGQIAHLSYVGDSVVGENCNLGAGTITAN
                     VRHDGRVVTSQVKGKKVESGRQKLGAILADGVHTGIHTSLYPGAKLWPQMTTLPGETV
                     RGDLLPEGSGWD"
     gene            3081..3248
                     /locus_tag="PKM23_11585"
     CDS             3081..3248
                     /locus_tag="PKM23_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92154.1"
                     /translation="MKCKVGQLIPLSDYGREGAPIMYKAWVCSNPECGYNLRIDNGEV
                     SRGREIRESSK"
     gene            3332..4288
                     /locus_tag="PKM23_11590"
     CDS             3332..4288
                     /locus_tag="PKM23_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92155.1"
                     /translation="MQKKSSAQEKQPFFQRYKIHIAATGFAVLIWFLVVSNDIYDHEI
                     RVPIDIPAVQSHYIITSELPTEARLRVRGQGMALLALRLFREGRLELNMEWGPGERVL
                     HPRAQDVILSGGAHAISVLQLLEPQEIPVVIEELASRILPIGNRMIVKPMAGYAVVGD
                     IAIDPSEVVARGPNSLIKKLTAIPTVERTFDKVKSPFTGEILLSSPDPRRIMLQPGRA
                     TFRVDIQKLMEKRIERIPVSVRNLPPGFRAMVLPATLSLVAEGGVSLIAPLTEKEISA
                     YIDYARQPETEGQNAPAYIVPLPGVRYRDISPKRFKVILERE"
     gene            4285..>5166
                     /gene="tsaD"
                     /locus_tag="PKM23_11595"
     CDS             4285..>5166
                     /gene="tsaD"
                     /locus_tag="PKM23_11595"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNY92156.1"
                     /translation="MMRVLGIETSCDETSAAVVDESGICSNIIATQKVHEAYGGVVPE
                     FASRAHMRQLSGIVRLALQQAGTSLAGIDALAVTCGPGLAGSLLVGLSLCKGIALTLG
                     KPFIGVNHIEGHILATAAQLHKIDYPYICLVASGGHTLLVLVQEPRRYEIVGRTMDDA
                     AGEAFDKVAKVLGLGYPGGPAVEASARGGNPDAIAFPRALSESTNLNFSFSGLKTAVL
                     YYAQSLDPVEGAARLPDIAASFQMAVIDTLFIKSRRALEACGCRTLVLAGGVMRNGPL
                     RKIFAARCGELGIALAIP"
BASE COUNT         1037 a         1599 c         1499 g         1031 t
ORIGIN      
        1 cgcccggcgc gttcgaggcc gaacggaccc cctgccatgc ggccgggccg gacgtcatca
       61 aaaattttat accattttac tgatttgtgc cgcgtttgtc aagcattttt atgctgcggg
      121 attcaccata aaaaaaccgc cccttgcgga ggcggttttg gacagcttgt tcggcgtacc
      181 ccgcgagcgg ctgcggcggg cggtcgtcag cgcagaaatt tgatccggcg cgagatctca
      241 taatcatcga cctgaagctt gtagatatag gtatccgagg acaagttgag gccgttgtcg
      301 tccttgccat tccaggagac ggtatgagtg ccacccggca gggcggcggc catcagcctg
      361 cgcacctgcc ggccgcgcag atcaaagatc gataacagca ccttgcccgc ctccttgagg
      421 cggaaattga tccgcgtctc gggattgaag ggattgggat agatctgctc gaggacataa
      481 tcgaccggcg ctgtggccga gactctcacg ggaccatgca tggtcatgcg cccgcgataa
      541 tccacatccg ccagcttgta gtagtagctc ttgcccggct cgacagtcga gtcgctgtac
      601 tcataggaac ggcgaaaagt cgagctgcca gcccccctta ccatgtttct ggtgatctgg
      661 ctgtagggtc cctcctccga gcacgcgcgg aagagatgaa agcccagatt gtcattttca
      721 gcctgggttt gccactcgag cctcaccgcc gcattctccg gatgggcctc gaaggagccc
      781 agggcgatcg gggtgagatc aaaacgataa tcctgaacct ctccggccgc atcgctcaag
      841 ccggccgggc tctgaaccgc attgggctgc cgactgctgc tgaagcggaa acgagcccaa
      901 acctcctggc cgggacggta cccctccggc caggcaaagc tgaagctgtt gtaaccggcc
      961 cgcaccggct ggggtgggat gatattgtcg gcagcctgcg aaaagtcaac cccgccctgc
     1021 tccccgccgt caaaatcgat ccatcccgcc atcacgccat cccggctgag gaaacaggtc
     1081 acctcgcgtc cgttaaagct gaactggatc ccctccccgt cggaatcatc ccgggcgacg
     1141 gactcaccgt caatccaggg accgccggag ggacgtccga tgaagacacc cggatcgtag
     1201 aggtgagccg cggcgggaag accctccgca ccgtaatcgc agagctgctg ggctgccagc
     1261 ggcccggcca gcagaaaaag caccatcgcc aggaaaaacc cggcagagcc tctcccatta
     1321 tgcatcccga cctcccgcct tgtggcgagc aatacccact gaatcacatt tttgtggcat
     1381 gacggcctca tcactcgccc tcacaaccca tatcagatta ttgttattat tagttttata
     1441 ttttttttca tcctcattca cctgatgatt aatcaaacag catgccacaa ggcaaaaaaa
     1501 aaggctgttc acaatgaaca gccttgatta tgccgacaag aatggaggcg gaggaaaaat
     1561 catgcgccgg tgaggatcaa tcccacccgc tcccctccgg aaggaggtcg ccccgcacgg
     1621 tctcgccggg cagggtggtc atctgcggcc agagcttggc gcccggatag aggctggtgt
     1681 ggatgccggt gtggactccg tcggcgagga tggcgcccag cttttgccgg ccgctctcca
     1741 cctttttacc cttgacctgg gaggttacga cacggccgtc atgacggaca ttggcggtga
     1801 tggtgccggc gccgaggttg cagttctcgc ccacgaccga atcgccgaca tagctgagat
     1861 gggcgatctg gccgccgggc atgatgatgc tgtttttgat ctcgaccgcg tggccgatgc
     1921 ggcagttgtc gccgatggcg gtgaacctgc gcacatagca gttggggcca atgtcgcagt
     1981 tgcggccgat ggccaccggg ccttcgatat agctgccgct gcggattacc gtgcccgcgc
     2041 cgacctggac cgcaccgtta aggaccgccc ccgcctcgat ccgcccctcg atccggctcg
     2101 agctcatgcc ggccattata aactcctgat gcttgagcag atcccaggcg aagccggtcg
     2161 gcagccagaa gccggtgatc gggacaatgc tgaaggtgct gcgctggacg gtcgccataa
     2221 tcgccccgat aatctcgatc tcgccgcgct cactcagctc cactttctcc agatcggcaa
     2281 atatgtcggg ctcgaagatg tagcagccga tgttggccag attgccgaga aaggttttgg
     2341 gcttctcgac catctgaatc agccgatagt gcgcgtccac caccgcgact ccgtagagag
     2401 agggatcggc caccggcttg accagggcgg cataggggtg ctccagcagt ttcaaaaaat
     2461 cctcgcgggc gaagaggtca tcaccgttgc aggcgacaaa acggccgcgg atatggggct
     2521 tggcctgcaa aatggcatga ccggtgccca gctgctgctt ctgctcctga tagatcaggt
     2581 ggatgcctcg atactcgtcg ccgagcaggg cgcggatcat ctcctggcgg tagccgacga
     2641 tcaggatgac ctcgtcaaag actccggcca tctggtcgag gctgtgatag atcaggggac
     2701 ggttggcgac cggaagcagc ggcttgggcc gagtcagggt cagggggtag gtgcgggtgc
     2761 tcttgcccgc caccatcatc actgcttgca tcagtttgct ctcctggaac cgttggacgt
     2821 tattatggag ctaatttaag caaaggaagc cttttatgaa agcatttttc aaaaaaatgg
     2881 aactgcaggg tgcagaattg taaaaaattg ttgattttca ttgccttata tcgtatattt
     2941 cacaccgtga acccttccct gcacaacagg gtcgtggtat gtctggaaac cggcaaaccc
     3001 cactggcgcc acctaatcaa tcataactgt gggatctatg aggtgaggta agggcaatgg
     3061 aaaattcctt tcccagttgt ttgaagtgta aagttggaca gttgattcct ttatcggatt
     3121 atggccgcga aggcgccccg atcatgtaca aggcctgggt gtgctcgaat ccggagtgcg
     3181 ggtacaacct ccgcatcgat aacggcgagg tcagccgcgg cagagagatc agagagtcga
     3241 gcaaatagct cacccttcac agtgaagtga attatcaggg ggaccaccgg tcgcccctct
     3301 tttttttggt gttcattttc ttataaacaa gatgcagaaa aagagttctg cgcaggaaaa
     3361 acagcccttc tttcagcgct ataaaatcca cattgccgcc accggcttcg ccgtcttgat
     3421 ctggttcctc gtcgtttcaa acgacatcta cgaccacgag atcagggtac ccatcgatat
     3481 tccggcggtt caatcccatt acatcatcac cagtgagctt ccgaccgagg cccgcctcag
     3541 ggtgcggggg cagggcatgg ccttgctggc cctgcggcta ttccgcgaag ggcggctgga
     3601 gctgaacatg gaatggggtc cgggcgagcg tgtgcttcat ccccgcgccc aggatgtcat
     3661 cctcagcggc ggcgcccatg ccatatcggt gctgcagctg ctcgagcccc aggagatccc
     3721 cgtggtcatc gaggagctgg cctcgcgtat cctgcccatc ggaaaccgga tgatagtcaa
     3781 gccgatggcg ggttatgccg tcgtcggcga tatagccatc gatccttctg aggtcgtggc
     3841 ccgggggccc aacagcctga tcaaaaagct cacggccatc cccaccgtcg aacgcacctt
     3901 tgacaaggtc aagagcccct tcaccggcga gatcctgctg agctccccgg atcccaggag
     3961 aatcatgctc cagcctggcc gggccacctt cagggtcgat attcagaaac tgatggagaa
     4021 acgcatcgag cgcattccgg tgagcgtacg caacctgccg cccggatttc gcgccatggt
     4081 gctgccggcc accctcagcc tggtcgccga gggcggtgtc agcctgatcg cccctctgac
     4141 cgaaaaggag atctccgcct atatcgatta cgcccgccag cccgagaccg agggccagaa
     4201 tgcccccgcc tacattgtcc ccctccctgg cgtacgctat cgcgatatct cccccaagcg
     4261 cttcaaagtc attctggaac gcgaatgatg cgcgtccttg gcattgagac ctcctgtgat
     4321 gagaccagtg cggccgtcgt cgacgaaagc ggcatttgct ccaacatcat cgcgacccag
     4381 aaggtccacg aggcctatgg cggggtcgtg cccgagtttg cctcccgggc ccatatgcgc
     4441 caactctccg gcatcgtccg gctcgccctg cagcaggccg ggaccagcct ggccgggatc
     4501 gacgccctgg ccgtgacctg cggcccggga ttggcgggtt cacttctggt cggcctctcc
     4561 ctctgcaagg gcatcgccct taccctaggc aaacccttca tcggcgtcaa ccacatcgag
     4621 gggcacatac tggcgactgc ggcccagctg cacaagatcg actatcccta catctgtctg
     4681 gtcgcctccg ggggtcacac cctgctcgtg ctggtgcagg agccgcgccg gtacgagatt
     4741 gtcggccgca cgatggatga cgccgcgggg gaggccttcg acaaggtcgc caaggtcctc
     4801 ggtcttggct atcccggcgg accggcggtg gaggcaagtg cgcgcggtgg caatccggat
     4861 gccatcgcct ttccccgcgc cttgtcagag agcaccaacc tcaatttcag cttcagcggc
     4921 ctcaagacgg cggtgctcta ttacgcccag tccctcgacc ccgtggaggg ggcggcccgc
     4981 ctgccggata tcgccgccag ctttcagatg gcggtcatcg acaccctttt catcaagagc
     5041 cggcgcgccc tcgaagcctg cggctgccgc accctggttt tggccggtgg tgtgatgcgc
     5101 aacggcccgc tgcgcaagat cttcgccgcc cgctgcggcg aactgggcat cgcgctggcc
     5161 atcccg
//
LOCUS       DAOPWU010000255         5165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16146_length_5165_cov_4.052055, whole genome shotgun sequence.
ACCESSION   DAOPWU010000255 DAOPWU010000000
VERSION     DAOPWU010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16146_length_5165_cov_4.052055"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2027)
                     /gene="hypF"
                     /locus_tag="PKM23_11600"
     CDS             complement(<1..2027)
                     /gene="hypF"
                     /locus_tag="PKM23_11600"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNY92157.1"
                     /translation="MNEERNRIERRRIRLEGIVQGVGFRPFTLRIARECGLTGFVRNE
                     SGGVLIEAEGSSFVLDQFLARLLSEHPPYARVVRHSVSPLPLGGEADFTIQFSTKGPA
                     EASFISPDLAVCPDCLAEMNNPAERRCRYPFLNCTRCGPRYSIVRSLPYDRSRTSMAD
                     FVMCPACRAEYDDPGDRRFHAQPTACPNCGPRLWLAGSGGEELSVPDPIAAVWRLLQE
                     GRIVAVRGIGGFHLCADARNGEAVETLRRRKGRGRKPFALMARDLATIRCWCEVNTEE
                     QALLKSTPRPIMLLRMAEPDESSAGGPERPQPLPWGVAPGQKYHGFMLPYTPLHHLLM
                     AGPLPVLIMTSGNLSEEPIAIGNEEAVARLGGVADAFLLHDREILQRSDDSIARVAAG
                     KTMVIRRSRGYVPDPILLREALPEPVLAVGGELKNTIALGRGSELFLSQHIGDLDNPL
                     ALDFFRHAIGHLQRLLRLEPQLLAHDLHPDYLSTQWALAQPAAKRFAVQHHHAHLASV
                     LAEHRLEGPCIGIILDGTGYGSDNTIWGGELLLGDASSFTREAWLEPLPLPGGAAAAR
                     EPWRMALSYLHAAYGRECLDLDLEVVHYRPREELELLLEAIEKGINSPLTSSCGRLFD
                     AISAIFGLCFINTFEAEAAMAVEMAAGPESGPRPPLEELLGPDFQPRGPLRL"
     gene            complement(2020..2718)
                     /gene="hypB"
                     /locus_tag="PKM23_11605"
     CDS             complement(2020..2718)
                     /gene="hypB"
                     /locus_tag="PKM23_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNY92158.1"
                     /translation="MSEIIPIGEKVLSDNDRLAAEIRARLARLNLPSLNLVSSSGSGK
                     TALLEKTLAHFGGRLRIALIAGDVQTDNDARRLAAAGGALVRPIVTGGACHLDARMVS
                     RVLAEMELEGCGLLIIENVGNLVCPSSYDLGEDKKVVLLSTAEGDDKPLKYPAIFRRA
                     ALLVITKTDLLGLSDFDLERAARHARMINPEIEILHLSSRSGEGMADWYAWLERLTRR
                     PAAAVPDAGGNPLE"
     gene            complement(2715..3062)
                     /gene="hypA"
                     /locus_tag="PKM23_11610"
     CDS             complement(2715..3062)
                     /gene="hypA"
                     /locus_tag="PKM23_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNY92159.1"
                     /translation="MHEISVAAALMRRVLAEKEARKLERISQIVLQIGPMSGIMPDAL
                     QFAYDSLREGTQLEGTELVIHSAELTTCCRHCLHLFDSRLMHTRCPRCGSEELQIMGG
                     DEFFIQYLVTEKV"
     gene            3322..>5165
                     /locus_tag="PKM23_11615"
     CDS             3322..>5165
                     /locus_tag="PKM23_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92160.1"
                     /translation="MSQTCTPDIVVSGYAETLSGTTMGYESPLPWLREALICRAGDGT
                     SSMAWRSEPLILPAGTRRVRLVWLAGLGCNLGEYSFTLALADRPLAEFTTKDEAEWQI
                     QGAGGAILAFRTIGSDRHRDRFGLMTLDLPAAGLKNGQALGLRITGSAAGSSGWVMTF
                     TGPIRQEFFLLPAGVLMRDGTQPVAVELLYLGDKGEAVIRAEGQPDHKLLLLFGRNSC
                     PLFYPAVHKETPRSVSVEIDGRSLTRTFVQKPVRPRTIYLVQHSHTDIGYTRPQAEIL
                     AEHLRYIDYALDFCDQTDHLPDDARFRWTCETSWAVREYLRSRPKAQIERLLRRVREG
                     RIEVTAQLLNWSELPDENALLHALEPLREFQALGLPVCTAMQNDVNGFAWCLTDYLNA
                     LGIRYVTSGINDARALKPFAVPTAFWWESPAGKRVLAFRADHYMTGNMLGLFSGDEGN
                     FVDSLTIYLNGLEERGYPHDALSLQFSGYFTDNAPPSTMASSAIEAWNNKYLWPRLRS
                     ATAREFLDHIAARAGDSPPVLRAAWPDWWTDGFGSAQRETAAGREAQAGMIANQGLFA
                     IARLAGIELPASVPGRIAGVMDQLNFWHEHTMGAAESISDPLAANSQE"
BASE COUNT          884 a         1672 c         1724 g          885 t
ORIGIN      
        1 agccgcagcg ggccgcgggg ctgaaagtcg ggcccgagga gttcttccag aggcgggcga
       61 gggccgctct ccggcccggc ggccatctcc acggccatag ccgcttccgc ctcgaaggtg
      121 ttgatgaaac agagtccgaa gatcgcggag atggcatcga agagacggcc gcagctcgag
      181 gtgagggggg aattgatgcc cttttcgatg gcctcgagga gcagctccag ctcttcccgc
      241 ggccggtaat ggaccacctc gaggtcgagg tcgaggcatt cacgtccata agcggcatga
      301 agatagctga gggccatgcg ccagggttcg cgggcggctg cggccccgcc cggcaggggt
      361 agcggctcga gccaggcctc gcgggtgaac gaggaggcgt cgccgaggag caactctccg
      421 ccccagatgg tgttgtcgct gccatagccc gtcccgtcga ggatgatgcc gatgcaaggc
      481 ccttcgagtc ggtgttcggc caggaccgag gccagatggg catggtggtg ctgcacggcg
      541 aatcgctttg cggccggctg tgcgagcgcc cattgggtcg agaggtaatc ggggtgaagg
      601 tcgtgggcga ggagctgcgg ctcgaggcgc agcagccgct ggaggtggcc gatggcgtgg
      661 cggaaaaagt ccagggccag gggattgtct agatcgccga tgtgctggct gagaaagagc
      721 tcgctgcccc gcccgagggc gatggtgttc ttgagctcgc ctccgaccgc gaggacgggc
      781 tcgggcaggg cttcccgcag caggatgggg tcgggaacat agccgcggct gcggcgtatg
      841 accatggttt tgccggcagc cacacgggcg atggagtcgt cgctgcgctg caggatctcg
      901 cggtcatgga gcagaaaggc gtcggcgacc ccacccaggc gcgcgaccgc ctcttcattg
      961 ccgatggcga tgggctcctc gctgaggttg ccgctggtca tgatcagcac cggcaggggg
     1021 ccggccatga gcagatgatg gaggggggtg taggggagca tgaagccgtg atatttttgg
     1081 ccgggtgcga ccccccaggg caggggctgc ggccgctccg ggccgccggc gctgctttcg
     1141 tccggctccg ccatcctcag gagcatgatc ggccgcggtg tgcttttgag cagggcctgc
     1201 tcttcagtgt tcacctcgca ccagcatcgg atagtggcga ggtcgcgggc catgagggcg
     1261 aagggcttgc ggccgcgccc cttgcgccgg cgcagggtct ccactgcctc cccgttccgc
     1321 gcatcggcgc aaaggtgaaa gccgccgatc ccgcgcaccg ccacaatccg gccctcctgc
     1381 agcaggcgcc agaccgcagc gatgggatcg ggaaccgaaa gttcttcgcc tcctgaaccg
     1441 gccagccaga ggcgcgggcc gcagttcggg caggcggtgg gctgggcgtg gaagcggcgg
     1501 tcgccgggat catcgtattc ggcccggcag gcgggacaca tcacaaagtc ggccatcgag
     1561 gtgcggctgc ggtcgtaggg gaggctgcgc acgatgctgt agcgcgggcc gcagcgggtg
     1621 cagttgagaa aggggtaacg gcagcggcgc tccgccggat tgttcatctc cgccaggcag
     1681 tctgggcaga ccgccagatc gggggagatg aaggaggctt ctgcggggcc ctttgtgctg
     1741 aactggatcg tgaaatccgc ctctcccccg aggggaagag ggctcacgct gtggcgcacc
     1801 acccgggcgt aggggggatg ctcactcagc agccgggcga gaaactgatc gagcacgaag
     1861 gagctgcctt cagcctcgat caggaccccg ccgctttcat tgcggacaaa gccggtgagg
     1921 ccgcactccc gggcaatgcg cagggtgaag ggcctgaatc ccaccccctg aacgatgccc
     1981 tcgaggcgga tgcggcggcg ttcgatcctg ttcctttcct cattcaagcg gattgccccc
     2041 cgcatcggga accgcggcag cggggcgcct cgtgagccgc tccagccagg cgtaccagtc
     2101 ggccatcccc tcgccgctgc ggctggaaag atggaggatt tcgatttccg gattgatcat
     2161 gcgggcatgg cgtgcggccc gctcgaggtc aaaatcggaa aggccgagca ggtcggtctt
     2221 ggtgatcacg agcagggcgg cgcggcggaa gatggccggg tatttgaggg gcttgtcgtc
     2281 cccctcggcc gtgctcagca gcaccacctt tttgtcctcg ccgagatcat aactggaagg
     2341 gcagaccagg ttgccgacat tctcgatgat gagcaggccg cagccctcca gttccatctc
     2401 cgccagcacc cggctcacca tgcgggcgtc gaggtggcag gcgccgccgg tgacgatggg
     2461 ccggaccagc gctccgccgg cggcggccag acgccgggcg tcgttgtcgg tctggacatc
     2521 cccggcgatg agggcgatgc gcaggcggcc gccgaaatgg gccagggtct tttccagcag
     2581 ggcggtcttg cccgatccgg acgaactgac cagattgagg gaggggaggt tcagacgggc
     2641 cagccgggcg cggatttcag ccgccaggcg gtcgttatcg gatagcacct tttcgccgat
     2701 ggggatgatc tcgctcatac cttctccgtg accagatact ggatgaaaaa ttcatccccg
     2761 cccatgattt gcagctcctc cgagccgcag cggggacaac gggtgtgcat caggcggctg
     2821 tcgaagaggt gcaggcagtg gcggcagcag gtggtcagtt ccgcgctgtg gatgaccagc
     2881 tcggtcccct ccagctgcgt gccctcgcgc agtgagtcgt aggcgaactg cagcgcgtcc
     2941 ggcatgatcc cgctcatcgg accgatttgc aggacgatct ggctgatgcg ttcgagcttc
     3001 cgcgcctcct tttcagccag aaccctgcgc atcaaagcgg ctgcaaccga gatctcgtgc
     3061 atggccttcc cctttcactg gaaattacaa agagtatgac aggaaagcaa gcgggaattg
     3121 cgctttgagt gtacaaccct gatcgggccg gggaaaggcc ttgcaattat tgaaaaaatg
     3181 gtgcaaattg gcagcacagg gtggaacggc cgcccggctg cctcgtccgg tgccgtgcgc
     3241 cgcactgtcc ggccaaatca cggagagaag atgctcctct tgtgcttctt gatattcttc
     3301 ctttcaggag cgagttcagc catgtcccaa acttgtaccc ctgacatagt cgtcagcggc
     3361 tatgcggaaa ccctgagcgg cacgaccatg ggctatgaat cccccctccc ctggctgcgc
     3421 gaggctctga tctgccgcgc cggtgacggc acctcctcca tggcctggcg gagtgagccc
     3481 ctcattctgc cggcgggaac gaggcgggtg cgcctggtct ggctcgcggg cctgggctgc
     3541 aacctgggcg agtactcctt caccctcgca cttgcagatc gtcccctcgc cgaattcacc
     3601 acaaaggacg aggcggagtg gcagatccaa ggcgctggag gggcgatcct tgcctttcgc
     3661 accatcggca gcgaccggca ccgggatcgt ttcggactga tgaccctcga tctgcccgcg
     3721 gccggcctga agaatggcca agccctcggg ctgcgcatca ccggctccgc cgccggcagc
     3781 agcggctggg tgatgacctt caccggcccg atcaggcagg aattctttct cctcccggcc
     3841 ggggtgctga tgcgcgacgg aacccagccg gtcgcagtcg agctgctcta tctcggcgac
     3901 aagggggagg ctgtcatccg ggctgaaggc cagcccgacc acaagctgct cctgcttttc
     3961 gggcgcaaca gctgcccgct cttttatccc gccgtccata aggagacccc ccgctcggtg
     4021 agcgtggaaa tcgacggccg ctccctcacc cgcaccttcg tgcagaaacc ggtgcggccg
     4081 cggacgatct atctggtcca gcacagccat accgacatcg gctatacccg cccccaggcc
     4141 gagatcctcg ccgaacacct ccgctatatc gattacgccc tcgatttctg cgaccagacc
     4201 gatcacctgc cggatgacgc ccgcttccgc tggacctgcg agacttcctg ggcggtgcgc
     4261 gagtatctca ggagccgccc caaagcccag atcgagcggc tgctcagacg cgtgcgggag
     4321 gggcgcatcg aggtcaccgc tcagcttctc aactggtcgg aactacccga cgagaacgcc
     4381 ctgctccatg ccctcgagcc cctgcgcgaa tttcaggcgc tgggactgcc ggtctgcaca
     4441 gccatgcaga acgacgtcaa cggcttcgcc tggtgcctga ccgattacct gaacgcgctc
     4501 ggcatccgtt atgtcacctc gggcatcaac gacgcccgcg ccctcaaacc ctttgcggtt
     4561 cccaccgcct tctggtggga gtcgccggcg gggaagagag tgctcgcctt ccgcgccgac
     4621 cattacatga ccggcaatat gctcggcctg ttctccggag acgaagggaa ttttgtcgat
     4681 tccctcacca tctatctgaa cggcctcgag gaacggggct atccccacga tgccctctct
     4741 ctccagttca gcggctactt cactgacaat gctccgccat cgaccatggc ctcgtcggcc
     4801 atcgaggcct ggaacaacaa gtatttgtgg ccgcgcctgc gcagcgccac cgcccgcgag
     4861 tttctcgacc acatcgccgc ccgcgcaggc gattccccgc cggtgctgcg cgccgcctgg
     4921 ccggactggt ggaccgacgg cttcggctcc gcccagcgcg aaaccgcagc cgggcgggag
     4981 gcccaggccg ggatgatcgc caatcagggg ctgttcgcca tcgcccggct ggcgggcatc
     5041 gaactgccgg cttccgtgcc cggccgcatc gcaggggtga tggatcagct taatttctgg
     5101 cacgaacaca ccatgggcgc cgccgagagc atctccgatc ccctcgccgc caacagccag
     5161 gaaca
//
LOCUS       DAOPWU010000256         5149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16213_length_5149_cov_3.894189, whole genome shotgun sequence.
ACCESSION   DAOPWU010000256 DAOPWU010000000
VERSION     DAOPWU010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16213_length_5149_cov_3.894189"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="PKM23_11620"
     CDS             complement(<1..1916)
                     /locus_tag="PKM23_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HNY92161.1"
                     /translation="MLTSRTLDEKMLILLRQGKSFFHIGAAGHEAAQIGAGLALIPGV
                     DWAFPYYRDLAFMLSWGTTPLEILLNFLAKADDPSSAGRQMPQHYGHKALRIVSQSSP
                     TGTQFLQAVGVAMGAHKSGAREVVYVSSGEGTTSQGDFYEAINWATRDRLPVLFFIQD
                     NAYAISVPRAQQGAGSSLYEMAMGYKSMHRHEVNGLDLTEVNKTVRHCAELARSGKGP
                     SLVVAHTVRLFSHSSSDDQRKYRAPEDLQESEAADPLISLAHLLLERRFATPEQLETL
                     QQEVKIEIDEAARVAEEAAEPDPATLLDHLYAAPVTAAPEPEQPAPGPPVVIVDALNH
                     ALAEEMDADERMLIYGQDVADAKGGVFTVTKGLSTRFGSERVFNSPLAESSIIGTAIG
                     LALYGFKPVVEIQFGDYIWTAMMQLRNEVATMHWRSAGNFSCPMVIRVPVGGYIHGGL
                     CHSQNIEGFFAHLPGLHICYPSTAGDAKGLLKEAIHQQDPVLFLEHKGLYRQSYAMSP
                     EPGPDYRLPFGKARLVREGRHLTVITWGMLVHKAMQAAQSLATEGIEAEIIDLRTINP
                     LDMEVILHSLAKTGRALVAHEDTLTAGFGAEIAALIADAAFEYLDAPVRRLAAADSPV
                     PYNSGLEAVMLPQP"
     gene            complement(2044..3438)
                     /gene="lpdA"
                     /locus_tag="PKM23_11625"
     CDS             complement(2044..3438)
                     /gene="lpdA"
                     /locus_tag="PKM23_11625"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNY92162.1"
                     /translation="MEKFDLAVIGSGPGGYVAAIRAAQLGLNTVVIEKAELGGVCLNW
                     GCIPTKALLNSATLLNHIKHADGFGIVTEGVSVDYAKVIKHSRSVAARLSKGIEFLFR
                     KNKITTIVGTARLTAPGTIKVIGADKNEQTIEAKNLLIATGARARAIPGVAFDGERII
                     SSREAMVLPERPASLVIIGAGAIGVEFAYFFASLGTKVSLVEMMPHILPIEDAEAVQV
                     VEKAFKKMGIDMFTSTRVDKVEKSGTGVRIHTSGKEESKILEGEKALIAIGVQGNIEN
                     LGLEELGVKTEKGHIVVDKKSYRSSVNGIYAIGDVIGPPWLAHVASAEGIAAVEGIAG
                     HPAVPVDYENVPGCTYCQPQVASVGLTEEKAREKGLEIKVGRFPIRANGKSMAMGESE
                     GFAKLIFDARYGELVGAHIVGAEATELLAELNLGKTLETTADEIMHTMHAHPTISEII
                     KEAAEEAYGHAIHI"
     gene            complement(3479..4666)
                     /locus_tag="PKM23_11630"
     CDS             complement(3479..4666)
                     /locus_tag="PKM23_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNY92163.1"
                     /translation="MKPVTIKPDVYWIGALHPDLRLFDIIMPTKNGTTYNSYLVKGEQ
                     VAIIDTVKDKFSPAFIERIAELTDFNRIAYIVVQHNEMDHSGSLAALLEKAPQAKVIC
                     SKPAARYVQNILNRPVQIRSVENGEVIDLGGKSLQFFPSPFIHWPDTMMTYLPEKRLL
                     FTCDLFGAHFCDSHLFNDQITRDTWPDFEHYFRSIMRPFKKSVRNALKKVDELDFDII
                     APSHGPILRSGLDKYLQAYQKWSEPLPQNDPRQLLIYYASAYGNTEKMAAEIARGATA
                     AGVTVKVYDTMEISLLDHLDRIEAADAIAIGSPTINGDAVKPVWEFLAGLATLDLKGK
                     IGASFGSMGWSGEAVSFIDQRLANLKLRVIQPGIQATLVPGPEELQKCHEFGAALAQA
                     MLT"
     gene            complement(4736..4939)
                     /locus_tag="PKM23_11635"
     CDS             complement(4736..4939)
                     /locus_tag="PKM23_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92164.1"
                     /translation="MEELKLYLVYHLGINPCWNLRVARSPEEALTSCFHHSDTVGIND
                     MDAGSCRVEEIRLEGYKIRIEKA"
     gene            complement(4957..>5149)
                     /locus_tag="PKM23_11640"
     CDS             complement(4957..>5149)
                     /locus_tag="PKM23_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNY92165.1"
                     /translation="IATSSMVTEMVKGKTLDEALQISNKAVAQSLGGLPKIKMHCSVL
                     AEDALKAAIDDYRKKKKEK"
BASE COUNT          974 a         1376 c         1663 g         1136 t
ORIGIN      
        1 tcgggttggg ggagcatgac ggcctcaagg ccgctgttgt agggcacagg agagtcggca
       61 gcagccaggc gccggaccgg agcgtcgaga tactcaaagg cggcgtcggc aatgagggcg
      121 gcgatttcag ccccgaatcc cgccgtcagg gtatcctcgt gggcgacgag ggcgcggccg
      181 gttttggcga gtgaatggag aataacctcc atgtcgaggg gatttatggt gcgcaggtcg
      241 atgatctcgg cctcgatgcc ttcggtggcg aggctttggg cggcctgcat ggccttgtgc
      301 actagcatgc cccaggtgat cacggtgagg tggcgtccct cacggacgag ccgggctttt
      361 ccgaagggga ggcggtagtc ggggccgggt tcaggcgaca tggcgtagct ctggcggtag
      421 agtcccttgt gttcaagaaa gaggaccgga tcctgctggt gtatggcctc cttgagcagt
      481 cccttggcat caccggccgt gctgggatag cagatatgga ggccggggag atgggcgaaa
      541 aagccctcaa tgttctgact gtggcagagt ccgccgtgga tgtatccgcc gaccggcacg
      601 cggatgacca ttgggcagga aaagttcccg gccgagcgcc agtgcatggt cgccacctca
      661 ttgcgcagct gcatcatggc ggtccagata tagtcgccga actggatttc gacgaccggc
      721 ttgaagccgt aaagggcgag gccgatggcg gttccgatga tgctggactc cgccaggggt
      781 gagttgaaga cccgctcgga tccgaagcgg gtggagagcc ccttggtgac tgtaaaaacg
      841 ccgcccttgg catcagcgac atcctgtcca tagatgagca tgcgctcatc ggcgtccatc
      901 tcctcggcca gggcatgatt gagggcatcc acgatgacca ccggggggcc tggcgcgggc
      961 tgttccggtt cgggagcagc agtgacgggt gcagcataaa gatgatcgag cagggtggcg
     1021 ggatcgggtt cagccgcctc ctccgctacg cgagcggcct catcgatctc gatcttgacc
     1081 tcctgctgca gggtctcgag ctgctcgggc gtggcaaagc ggcgttccag caacagatgg
     1141 gccaggctaa taaggggatc cgcggcctcc gattcctgaa gatcctccgg ggcgcggtat
     1201 ttgcgctggt catccgagga tgagtgggag aagagacgga ctgtatgggc gaccaccagg
     1261 ctcggtccct tgccgctgcg ggcgagttcg gcgcagtggc gtacggtctt gttcacctcg
     1321 gtcaaatcca ggccgttcac ctcatgacga tgcatgcttt tataccccat cgccatttcg
     1381 tacagggagc tgcccgctcc ctgctgggcg cgcggcacag agatggcgta agcattatcc
     1441 tggatgaaaa agagcaccgg cagcctgtcc cgggttgccc agttgatggc ctcgtagaaa
     1501 tccccctggc tggtcgtgcc ctccccggag gagacataga ccacttcccg ggcgccgctc
     1561 ttgtgtgccc ccatggccac gccgacggcc tggaggaact gggtaccagt gggactggat
     1621 tgtgagacga tgcgaagggc tttatggccg tagtgctggg gcatctgccg tccggcggag
     1681 gagggatcat cggctttggc gagaaaattg agcaggatct ccaggggtgt cgtaccccag
     1741 gagagcatga aagcgaggtc gcggtagtaa gggaaggccc agtcgacccc cgggatcaag
     1801 gcgaggccgg cgccgatctg ggcggcctcg tggccggcgg cgccgatatg gaaaaaactc
     1861 ttcccctgcc gcaaaaggat gagcattttt tcatccaggg tgcggctggt gagcatcagg
     1921 cgatagctct tcaggagatc cggtttagga aaagaagggc catggctcac aaaacaagct
     1981 tcctttcacg acgtacaagt cgctccggcg ccgccggggc caccggagtg gtgcgacggt
     2041 caactagatg tggatggcat ggccataggc ctcttcagcg gcttccttga taatttccga
     2101 tatggtcggg tgggcgtgca tggtatgcat gatctcatcg gctgtggtct caagggtttt
     2161 gccgaggttc aactcagcca ggagttcggt ggcttcggcg ccgacgatat gggcgccgac
     2221 caattcgccg tagcgggcat cgaagatgag cttggcgaag ccctcgcttt cgcccatggc
     2281 catggacttg ccgttggccc ggattggaaa gcggccgacc ttgatctcga gccctttctc
     2341 gcgcgctttc tcctcggtga gccccacgct ggccacttgc ggctggcagt aggtgcagcc
     2401 cggaacgttt tcataatcta ccggaactgc gggatggccg gcgatgcctt cgacggcggc
     2461 gatgccctca gccgaggcga cgtgagcgag ccagggcggt ccaatgacgt caccgatggc
     2521 atagatgccg ttcaccgagc tccggtagct ctttttatcg accacaatgt ggcccttttc
     2581 ggtcttgacc ccgagctcct ccaggccaag attttcgata ttgccttgta ctccgatcgc
     2641 gatgagggcc ttctccccct ccaggatctt gctctcttcc ttgccggagg tatggatcct
     2701 gacccctgtg ccactctttt ccactttatc aacgcgggtg ctggtgaaca tgtcgatgcc
     2761 catcttttta aaggcctttt cgaccacctg aacggcctcg gcatcttcga tcgggaggat
     2821 atggggcatc atctcgacga gactgacctt ggtgcccaga gaggcgaaga aataggcaaa
     2881 ctcaactcca atggcgccgg cgccgatgat gaccaggctt gcggggcgtt ctggcagtac
     2941 catggcctcg cggctgctga tgatgcgctc accgtcaaag gcgacgcctg ggattgcgcg
     3001 ggcgcgggct ccggtggcga tgaggaggtt cttcgcctca atggtctgct cgttcttgtc
     3061 cgcgccgatc accttgatag tgccaggggc ggtcagacgg gcagtgccaa cgatggttgt
     3121 gatcttgttc ttgcggaaaa ggaactcgat ccccttggag aggcgtgctg caacactgcg
     3181 actgtgcttt attactttgg cgtaatcgac cgacactcct tcggttacga tcccgaatcc
     3241 atcagcatgt ttgatatgat tgagcagggt ggcgctgttg agaagggcct tggtcggaat
     3301 gcagccccag ttaaggcaga cgccgcccag ttcagccttt tcgatgacca ctgtgttgag
     3361 gcccagttgg gcagcccgaa tggcggcgac gtagccgccg ggccctgaac cgatgactgc
     3421 gagatcgaat ttttccatat tgggatcctg actaatggag cggttctggt tcaagaaatc
     3481 acgtgagcat ggcctgggcg agagcagccc cgaactcatg acatttttgc agttcttccg
     3541 gtccagggac aagggtggcc tggatgccgg gctggatcac cctgagcttg agattggcga
     3601 ggcgctggtc gatgaaggaa acggcctctc cgctccaacc catggagccg aagctggcac
     3661 cgattttacc tttgaggtcg agggtggcga ggccggcgag aaactcccag accggtttta
     3721 cagcatcgcc gttgatggtg gggctgccga tggcgatggc atcggcagcc tcgatgcggt
     3781 cgaggtgatc gagaaggctg atctccatgg tatcgtagac cttgacggtg acgccggcgg
     3841 cagtggcgcc gcgggcgatc tccgccgcca ttttttcggt attgccataa gcgctggcgt
     3901 aataaatgag gagctggcgg ggatcgttct gcggcagggg ttcagaccac ttttgatagg
     3961 cctggaggta tttgtccagt ccgctgcgca ggatcggccc gtggctgggg gcgatgatgt
     4021 cgaaatcgag ttcgtcgacc tttttaaggg cattgcgcac cgacttttta aagggccgca
     4081 tgatgctgcg gaaatagtgt tcaaaatcgg gccaggtgtc gcgggtgatc tggtcattga
     4141 agaggtggct gtcacaaaag tgggcgccga aaaggtcgca ggtgaagaga aggcgcttct
     4201 ccggcaggta ggtcatcatg gtgtcgggcc agtggatgaa gggggaggga aaaaattgca
     4261 ggcttttacc gccgagatcg atcacctcgc cgttttctac cgatctgatt tgcacagggc
     4321 ggttgaggat gttctggacg tagcgggcgg cgggtttcga gcagattacc ttggcctgag
     4381 gagccttttc cagaagtgcc gccaggctgc cggaatggtc catttcgtta tgctggacaa
     4441 cgatataggc aatgcggtta aagtcggtca gttcggcgat acgttcgata aaggctggac
     4501 tgaacttgtc cttgactgta tcgatgatcg cgacttgttc tcccttgacc aaatagctgt
     4561 tgtaggtcgt cccgtttttg gtcggcatga tgatatcaaa gaggcgcagg tcgggatgga
     4621 gtgcgcctat ccagtaaaca tcaggcttga tcgtaaccgg cttcatctga aaacctcctt
     4681 tataccttag ctatcaactc aaaaggagcg cataatattt catcgcaagt gcgttctagg
     4741 ctttttcaat ccttatcttg tagccttcca gcctgatctc ctcgacgcgg cagctgcctg
     4801 cgtccatgtc gttgatcccg acggtatcgg agtgatgaaa gcaggaagtg agcgcctctt
     4861 cgggagagcg ggcgacgcga agattccagc agggattgat tcccaggtgg tagaccagat
     4921 agagtttgag ttcttccatc ctgcagctcc tttttattat ttctcttttt tcttcttgcg
     4981 ataatcgtca atggccgcct tgagggcgtc ctcggcgagg acgctgcagt gcattttgat
     5041 cttggggaga cctccgaggg actgggccac cgccttgttg gaaatttgca gggcttcatc
     5101 gagggtcttg cccttgacca tttcggtcac catcgagctg gttgcgatg
//
LOCUS       DAOPWU010000257         5140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16248_length_5140_cov_4.467257, whole genome shotgun sequence.
ACCESSION   DAOPWU010000257 DAOPWU010000000
VERSION     DAOPWU010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16248_length_5140_cov_4.467257"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKM23_11645"
     CDS             <1..254
                     /locus_tag="PKM23_11645"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNY92166.1"
                     /translation="ASYTTQSLTLKPGETLFFYSDGLVEARNLEGHFWGEERLRACLA
                     AMTHGSADAIGEKLVNRLQAFVGDAPTHDDITMIILQKL"
     gene            complement(302..1903)
                     /locus_tag="PKM23_11650"
     CDS             complement(302..1903)
                     /locus_tag="PKM23_11650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNY92167.1"
                     /translation="MVRKIVLMLLAAGALGAAELESRIVRVTVYPGAALVEREADLNL
                     KAGANTFTLAGLSAVLVDESVHARLISGRGLAIEEIKVERWFLSRADEGEARKVETAI
                     LDLERQIMLLEGELKSLAAKEKFLHSIQASNPAGQTAAFRPDAAAWNTTLTYLGSQLN
                     KLYEEMTALELKRRGLKEEKEALDKQLAELQSVKPREDKSIELTLRADGGGTGRLALT
                     YLVSEVSWWPTYEIRALPGEGRMELLYSGQVRQKSGEEWNEVELALSTAQPARGATVP
                     ELYPWELRLWEPSRAALQKAGGRAENAVMAAAYDKVEEEMAPPAPAGIESRSTSLLFA
                     IPGKRTVASGDEPARVLISRSDYAAQMSYITIPKLSSFVYLQGKIVNEGLAPLLAGQA
                     MIYVDGDYVGKTGIKPLAPGESADLSLGIDEGIKIKRELVKKYERSKGTLSKKQEMEY
                     DYRIVVENFKKQAVKVKVVDQLPRPLQEEIELGDVKLSPEPGEWDKEKQQMSWPMEIK
                     PGGKSEITLHFTVSYTRNGRVIGLD"
     gene            2194..3312
                     /locus_tag="PKM23_11655"
     CDS             2194..3312
                     /locus_tag="PKM23_11655"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNY92168.1"
                     /translation="MPDPRFAALARTIVRHSLRLQPGERFLIEATDIPPEMVIALIRE
                     ARSAEAVPLLNLKQNLVLREFYRLSDAAGLEEMGRLEASQMAGVQAYAGMRGSHNSAE
                     FSDVPEEQMRLYQKLWWQPVHQAIRIPGTRWVVLRWPHPAMAQLAQQSTEAFEDFFFS
                     VCTLNYGNVAAAMAPLVQLMAATDRVHIEGPGTDLHFSIRGMPVIPCAGEYNLPDGEV
                     YTAPVKESVEGRIRFTARSIYRGVVHEGIELEIEEGRITAARSDKSELLNQVLDTDPG
                     ARFIGEFALGVNPHIRRPLLDILFDEKIAGSFHFTPGSCLDNTPNGNQSVIHWDMVCI
                     QTPEFGGGSIRFDNRLIRQDGRFIVPELYPLNPENLLV"
     gene            3328..4410
                     /locus_tag="PKM23_11660"
     CDS             3328..4410
                     /locus_tag="PKM23_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92169.1"
                     /translation="MKNDPVAAARKAIAEKEYAQAIELLLPAAAGEKMNEEALLLQAR
                     CDLALHHHAAAGKIYSFMLQQGAPFSPAARAEAALILGQPQTSLALLAPLATGDLTGE
                     AALIAAVSAYCCGRISDCMRYLARFAAAGEEWDEEDPVELVIEHALERSEYHDLEQIY
                     LDAQESAGKPGPQPRNRWFAINIPVYELYTASRPDKRLKRAAALVRVLAPGEPFSPEG
                     ATERLRGILQDFAGSEEDARFGLESLKHLEAGSWAELARMIMALQLEHLRQFAGSLGL
                     EGERIATGALQQLIPLLPLRPAMGLMLLYAIADSEDRMLQQMVQNIEEEVLAALIQVA
                     FQAFYLEMERIRLLDLPPPPLEPDLP"
     gene            4507..>5140
                     /locus_tag="PKM23_11665"
     CDS             4507..>5140
                     /locus_tag="PKM23_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNY92170.1"
                     /translation="MLSPFHNESFTDFSKPENRQKQQEALKLVASQSAREYPLFIGGR
                     DVTTGRTFKSMNPSHTSQVVGTFHKAGQAEVDLAMKAALEAFEKWRWVAPKERAIILL
                     KAAAVMRRRKLEVNAWMITEIGKSWPEADADTSEAIDFLEFYAREALRWSEVKGATPF
                     PNEFPELNYIPLGVGAIIPPWNFPMAILTGMTAAAIVTGNTVLLKPASDTP"
BASE COUNT          952 a         1758 c         1497 g          933 t
ORIGIN      
        1 atgccagtta tacgacccaa agcctcaccc tcaaaccggg cgagaccctc tttttctact
       61 cggatggatt ggtggaagcc cgcaatctgg aggggcactt ctggggagag gaacgattgc
      121 gcgcctgcct ggccgctatg acccacggca gcgccgacgc catcggtgaa aagctggtca
      181 accgcttgca ggcctttgtc ggtgacgccc cgacccacga cgacatcacc atgattattc
      241 tgcaaaaact ctaggctgcg ccggataggg ccggcgcagc cgtcaatcca tcgcctgtgg
      301 ttcaatccag tccgatcacc cggccgttgc gggtatagga gacggtaaag tggagggtga
      361 tctcgctttt gccgcccggt ttgatctcca ttggccagct catctgctgc ttttccttgt
      421 cccactcccc cggctcaggt gaaagtttga catcgccgag ttcgatctcc tcctgcagcg
      481 gccgcggcag ctgatccacc actttcacct tcaccgcctg cttcttgaaa ttctcgacca
      541 caatccggta atcgtactcc atctcctgct tcttgctcag ggtccccttg ctgcgctcgt
      601 actttttcac cagctcccgt ttgatcttga tgccctcatc gatgccgagt gagagatcgg
      661 cgctctcgcc gggggcgagg ggcttgatgc cggtcttgcc gacataatcg ccatcgacgt
      721 agatcatcgc ctgtccggcc aggaggggag ccagcccctc attgacaatt ttgccctgca
      781 gatagacgaa ggaggagagc ttggggatgg tgatatagct catctgggcg gcgtagtcgc
      841 tgcgggagat caggacccgg gccggttcgt ctccgctggc gaccgtgcgt ttgccgggga
      901 tggcgaagag cagcgaagtc gaccggctct cgatcccggc cggggcaggg ggcgccatct
      961 cctcctcgac cttgtcataa gctgcggcca tgaccgcatt ttccgcccgg ccgcccgcct
     1021 tttgcagggc ggcgcgtgaa ggctcccaca ggcgcagctc ccaggggtag agctcgggca
     1081 cggtcgcgcc gcgggcgggc tgggcagtcg agagcgccag ctcgacctca ttccactcct
     1141 cgccgctctt ctggcgcacc tgtccggaat agagcagctc catccggcct tcacccggca
     1201 aggcgcggat ttcgtaggtg ggccaccacg agacctcact gaccagatag gtgagggcga
     1261 gccggccggt cccgccgcca tccgcgcgca gggtcagctc gatcgacttg tcctcgcgcg
     1321 gtttgaccga ctggagttcc gcgagctgct tgtccagagc ctccttttcc tccttcagcc
     1381 cgcgccgctt cagttcgagt gcggtcatct cctcgtaaag cttgttcaac tggctgccga
     1441 ggtaggtgag ggtggtattc caggcggcgg cgtccggcct gaaggcggcc gtctgccccg
     1501 caggattgga ggcctggatg gagtgcagga acttttcctt cgcggcgaga ctcttgagct
     1561 ctccctcaag cagcatgatc tggcgctcca gatcgagaat ggccgtctcc accttgcggg
     1621 cctcgccctc atcggctcgg gagaggaacc agcgctcgac cttgatctcc tcaatggcca
     1681 ggccgcggcc ggagatcagc cgggcatgca cagactcgtc caccagcacc gccgacagac
     1741 cggcgagggt gaaagtattc gcgcccgcct tcagattcag atcggcttcc cgctccacca
     1801 gggccgcgcc gggatagacg gtcacccgga cgatccggct ttccagttcg gccgcgccga
     1861 gggcgccggc cgccagcagc atcaatacga tttttctgac catgacaagg ctccgaaggt
     1921 gaaatgaaga tcccgatacc gccacccggg gaggccgtcc atgcggcccc accccttgta
     1981 aggtataaaa agacgcgaca aatatcaaga ccaaagtgaa gtggggaacg cggcgcaaga
     2041 tgccgggtgg taaccagatt tcggggggaa cgcagcgcaa gatgccgggt ggaagcagat
     2101 ttcgggggag ctccgcaccg gctgttgaca atggcccgtc atttgcgtat atttaacgga
     2161 aattcacccg ccatcgccca ttacggaaaa aacatgccgg atcctcgttt tgccgcactc
     2221 gcccgcacca tcgtccgcca ctccctccgg ctccagccgg gtgagcgctt cctcatcgag
     2281 gcgaccgaca tccccccgga gatggtgatc gccctcatcc gcgaggcgcg gagcgcggag
     2341 gcggttcccc tgctcaatct caagcagaac ctggtgctgc gggaattcta ccgtctcagc
     2401 gacgccgccg ggctggagga gatggggcgg ctggaggcct cccagatggc cggggtgcag
     2461 gcctacgccg gtatgcgcgg ctcccataac agcgccgagt tctccgatgt cccggaggaa
     2521 cagatgcgcc tctatcagaa gctgtggtgg cagccggtgc atcaggccat ccgcatcccc
     2581 gggacccgct gggtggtgct gcgctggccc catcccgcca tggcacagct ggcccagcag
     2641 agcaccgagg cctttgagga tttcttcttt tcggtctgta ccctcaatta cggcaacgtg
     2701 gccgcggcca tggcccctct ggtgcagctg atggcggcga ccgaccgcgt ccacatcgaa
     2761 ggaccgggga ccgacctgca cttttccatc cgggggatgc cggtcatccc ctgcgccggc
     2821 gagtacaatc tgcccgacgg tgaggtctat accgcccctg tcaaagaatc ggtcgagggg
     2881 cgcatccgct tcaccgcccg cagcatctat cgcggtgtgg tgcacgaggg cattgaactc
     2941 gaaatcgagg aggggcgcat caccgccgcc cgttcggaca agagcgaact gctcaaccag
     3001 gtgctcgaca ccgaccccgg cgcccgcttc atcggcgagt tcgccctcgg ggtcaatccc
     3061 catatcagaa ggccgctgct cgatatcctc ttcgatgaaa aaatcgccgg ctcgtttcat
     3121 ttcacgccgg gaagctgcct ggataacacc cccaatggca accagtccgt catccattgg
     3181 gatatggtct gcatccagac ccctgaattc ggcggtggtt cgatccgctt cgacaaccgt
     3241 ctcatccgcc aggacggccg ctttatcgtg ccggagcttt acccccttaa tccggaaaat
     3301 ttgctcgtgt aaagaggagc aggcaccatg aaaaatgatc ccgtcgccgc agcgcgcaag
     3361 gccatagcgg aaaaggagta cgcccaagcg atcgaactgc tgctgccggc ggccgccggg
     3421 gaaaaaatga acgaggaggc cttgctcctg caggcccgct gcgatctggc cctgcaccac
     3481 cacgccgccg ccggcaaaat ctacagcttt atgctccagc agggagcgcc cttctcaccg
     3541 gccgcccgcg ccgaggccgc cctcatcctt ggccagcccc agaccagcct ggccctgctc
     3601 gcccccctcg ccacgggcga cctcacgggc gaagcagccc tgatcgctgc cgtcagcgcc
     3661 tactgctgcg gccgcatctc tgattgcatg cgctacctcg cccgcttcgc cgcagccggc
     3721 gaggaatggg acgaggagga tcccgtcgag ctggtgatcg agcacgccct cgaacgcagc
     3781 gagtaccacg atcttgaaca gatctatctc gacgctcagg agagcgccgg caagcccggc
     3841 ccccagccgc gcaaccgctg gttcgcgatc aacatccccg tctatgagct ctataccgcc
     3901 tccagacccg ataaaaggct gaaacgagcc gccgccctgg tccgcgtcct ggcccccggc
     3961 gagcccttct ccccggaggg ggcaacggag cggctgcggg gcatcctgca ggattttgcc
     4021 ggcagcgagg aggacgcccg cttcggcctc gaaagcctca agcacctcga ggccggcagc
     4081 tgggccgaac tggcccgcat gatcatggcc ctgcagctcg aacacctgcg ccagttcgcc
     4141 ggcagcctcg gactggaggg cgagcgcatc gccaccggcg cgctccagca gctcatcccc
     4201 ctcctccccc tgcggcccgc gatgggactg atgctccttt acgccatcgc cgacagcgag
     4261 gaccgcatgc ttcagcagat ggtccagaac atcgaggagg aggtgctcgc cgccctcatc
     4321 caggtcgcct tccaggcctt ctacctggag atggaacgca tccgtctcct cgacctcccc
     4381 cctcctccac ttgagccaga tttgccttga ctttcaagct ttaaagacct aaaataaggc
     4441 gtgaaaacgt gcagcgggcc ccaccctccc gctgcgcccg tctgacccca ttccaggagg
     4501 agccttatgc tttccccttt tcacaatgag tctttcactg atttcagcaa gccggaaaac
     4561 cggcaaaaac agcaggaagc cctgaaactg gtcgccagcc agtccgcccg ggagtatccc
     4621 ctcttcatcg gcggccggga tgtgaccacc ggcagaacct tcaagtcgat gaacccctcc
     4681 cacaccagcc aggtggtggg gacctttcac aaggccggac aggccgaggt cgatctcgcc
     4741 atgaaggccg ccctcgaggc ctttgaaaaa tggcgctggg tcgcccccaa ggagcgggcc
     4801 atcatcctgc tcaaggcggc ggcggtgatg cgacggcgca agctcgaggt caacgcctgg
     4861 atgatcactg aaatcggcaa aagctggccc gaagccgacg ccgacacctc cgaagcaatt
     4921 gatttcctcg aattctacgc ccgcgaagcc ctgcgctgga gcgaggtcaa gggcgccact
     4981 ccatttccca acgagttccc cgaactcaac tatatccctc ttggcgtcgg cgccatcatc
     5041 ccgccctgga attttccgat ggccattctc accggcatga cggccgcggc catcgtcacc
     5101 ggcaataccg tgctgctcaa gccggcctcc gacaccccct
//
LOCUS       DAOPWU010000258         5133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16276_length_5133_cov_4.329854, whole genome shotgun sequence.
ACCESSION   DAOPWU010000258 DAOPWU010000000
VERSION     DAOPWU010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16276_length_5133_cov_4.329854"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PKM23_11670"
     CDS             <1..1134
                     /locus_tag="PKM23_11670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNY92171.1"
                     /translation="QISRRGFLRDAAAAGLLLGAASLPASAPAQDKEKKEEPAIRHRT
                     LGKTGLEMTILGYGAMRTSDPAVIRQAIDMGINNIDTARGYMDGHNEEIVAKAVGELR
                     KQVVITTKIRVGTKERMSADLDASLAALNTDYIDILLLHGLKRDEEVLNEEWIGMLTE
                     AKKAGKVRFAGFSTHNNMAGTLRAAVKGGFFDVVLTAYNFKSEPDLTKAIAEAAKAGI
                     GIIAMKTQAGGYTDAGMGGLNPHQAALKWVLEDQNVACAIPAMVTFDQLNENFRVMGS
                     HFGWMDRKTLHRYGRVIDDRLCRFCNRCAEQCTHGVAISDIQRCLMYAEGYHDQSLAL
                     SSYTALGREADLSACRSCSGCTVHCPNGVNIAANLQRAMQLFA"
     gene            1144..2019
                     /locus_tag="PKM23_11675"
     CDS             1144..2019
                     /locus_tag="PKM23_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92172.1"
                     /translation="MKRILFLLLALCALCAAQEMPLTDRLPLPASGSGWRFDIEPQSY
                     LPDNLFEYINGEAELYNDYHFAGMATAAYIRGDDMNMTYSVDISDMGTPLDAFGLYSR
                     HRSPELEYAAIGEEATVSELNIRFFKGRYFVQINAGSFEEAVREEMLRTANAIAETLP
                     VAPMPRELALLPQENQLPHSLVYYTRGMLGQSLFPAGLMAKFVVASDTVGAFLVLTSS
                     AGESLPAFQGFSASLKERGSLLAESPGRIEVETPYQGRILALHHLHWIVGVMGYKERE
                     TAERLAAGLIANLGE"
     gene            2019..3257
                     /locus_tag="PKM23_11680"
     CDS             2019..3257
                     /locus_tag="PKM23_11680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY92173.1"
                     /translation="MKVLIVNTLWGGHPGGIDRYISQIIHMLHRAGERTAMVYGRRRG
                     EPMERNLPLEGEEALPGLERAPEEKNNRVAERLLTLAAGMQPDVVFVQDIAHAPALSA
                     LCRSFPVVSMFHDYRPICLKDTRRDYFARRLCEHPLGWRCLLKGHFIRRPASGSHLPR
                     LASLQEAQRLLNAYQGCHHALTASEFVRSAYLLNGFAPHRIATLPLFTPENGFDPAGS
                     YPREKILLFVGRLTDRYKGADLLLEVMERCRAEIRLHIVGGGRYTARIKSLCAKKELG
                     HRVKLMGWMEGEALQRAYRQAMAVVMPSLWAEPFGLVGLEAMANGTPVVAFDVGGIPQ
                     WLRENVTGFLVPWLDTAAMAERIDRLAGNPVLVRQLGLAGHHHVRSEFSESAYLEGLM
                     SAFRLAISRFHEERGGSLPS"
     gene            complement(3243..4763)
                     /locus_tag="PKM23_11685"
     CDS             complement(3243..4763)
                     /locus_tag="PKM23_11685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNY92174.1"
                     /translation="MNPAPEQPDTDAGGELFFRSTLAGRVTGAALWIAGFYFLAAFLF
                     LAWRHIPWPFELEWMEGGMLDHVHRLLEGGRLYGPPALDFTPFSYPPLYFHLAVLLGR
                     LFGPGLALLRGISTLALLLTVFIIGQWTARESGSRHAGLLAGTLYLASYPVSGFWFGL
                     ARVDSLFLLLLLAGLCLLRCSRTLAGGLGAAILLALAFFCKQTALIITLPVMVWLILF
                     GEGPRRWLVPAAWLILLGGGLWTLQRASDGWLFYYLFYLPGQHVPVWWRVHHFWIDYF
                     FKPFPIATLAAGLSLFLGPGRLHGPRLFWLAAAAGLIGGPWLASVPSGAFHNVAMPAH
                     AALAILFALAVQRWFARAVRPLLPWAAALLQLLLLLYNPCHHLPAPADRAAGEALVGR
                     IAAVEGEVWFPSHGYLSRMAGKSGCAHRCALDDVLRGKDEPGRRRLVDEIDTALARRR
                     FEVIITNDDWLAREIKGGYGEGETIFTRPCLFWPLTGWQTRPEVWYQQRGDDSGGE"
     gene            complement(4760..>5133)
                     /locus_tag="PKM23_11690"
     CDS             complement(4760..>5133)
                     /locus_tag="PKM23_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92175.1"
                     /translation="RPHRAVGLWLREHARPGEKLVVNEIGAIGWYSNLPVIDMIGLTN
                     KQIPGFWKSGDFAGYADFILAQRPVWIALNDRQEPGDEGMDPVHRALYAHMQADGSFQ
                     LVRTFPLRRDKNLLLFRRNGP"
BASE COUNT         1004 a         1700 c         1594 g          835 t
ORIGIN      
        1 cagatcagcc gcagaggttt tctcagggat gccgcggccg cagggctgct cctcggcgcc
       61 gccagcttgc cggcctctgc cccggcccag gacaaggaga agaaagagga accagccatc
      121 cgtcatcgca ccctcggcaa aaccggcctg gagatgacca tcctcggcta tggggccatg
      181 cgcacctccg atcccgccgt catccggcag gccatcgata tgggcatcaa caacatcgat
      241 accgcacgcg gctatatgga cggccacaac gaggagatcg tcgccaaggc cgtcggcgaa
      301 ctgcgcaagc aggtggtcat caccaccaag atccgcgtcg gcaccaaaga acggatgagc
      361 gccgacctcg acgccagcct cgccgccctc aataccgatt atatcgatat cctgctcctg
      421 cacggtctca aacgcgacga ggaggtgctg aacgaggagt ggatcggcat gctgaccgag
      481 gccaaaaaag cgggcaaggt gcgcttcgcc ggcttttcga cccacaacaa catggccgga
      541 accctgcgtg cggccgtcaa gggcggcttt ttcgatgtcg tcctcaccgc ctacaatttc
      601 aagagcgaac ccgatctcac caaggcgatc gccgaggctg ccaaggccgg catcggcatc
      661 atcgccatga aaacccaggc cggcggctat accgacgccg gcatgggcgg ccttaatccc
      721 caccaggccg ccctcaagtg ggtgctcgag gaccagaacg tcgcctgcgc tatcccggcg
      781 atggtcacct tcgaccagct caacgaaaat ttccgggtga tgggctccca ctttggctgg
      841 atggatcgta aaaccctcca ccgctacggc cgcgtgatcg acgaccgcct ctgccgcttc
      901 tgcaaccgct gcgcggaaca gtgcacgcat ggcgtcgcca tcagcgatat ccagcgctgc
      961 ctgatgtacg cggagggcta ccacgaccaa tccctcgccc tctcctccta caccgccctg
     1021 ggccgggaag ccgatctcag cgcctgccgg agctgctccg gctgcaccgt gcattgccca
     1081 aacggggtca acatcgccgc caatctgcag cgcgccatgc agcttttcgc ctgaggcaac
     1141 gtgatgaaaa gaatactttt tctcctcctc gctctctgcg cgctctgcgc tgcccaagag
     1201 atgcccctga ccgaccgcct gcccctgcct gcgagcggaa gcggttggcg ctttgacatt
     1261 gagccgcaga gctacctgcc ggacaatctc ttcgagtata tcaacggcga ggccgagctg
     1321 tacaacgact atcattttgc cggaatggcc accgccgcct atatccgcgg ggatgatatg
     1381 aacatgacct actcggtcga tatcagcgac atgggcaccc ccctggacgc attcggcctc
     1441 tacagccgcc atcgttcgcc ggagctcgag tacgctgcca tcggagagga ggccacggtc
     1501 tcggaactga acatccgctt cttcaagggg cgctatttcg tccagatcaa cgccggctcc
     1561 ttcgaagagg cggtgcggga ggagatgttg cgcacggcga acgccatcgc cgaaaccctg
     1621 cctgtggcgc caatgccgcg cgaactcgct ctccttccgc aggaaaacca gctgccccat
     1681 tccctggtct actacacccg gggcatgctc ggccagtccc tcttcccggc agggttgatg
     1741 gcaaaattcg ttgtggcgtc tgataccgtg ggagcctttc tcgtcctcac ctcctccgcc
     1801 ggcgagtcac tgccggcttt ccagggcttt tccgccagtc tgaaggagcg gggcagcctg
     1861 ctcgccgaaa gcccgggccg gatcgaggtg gagacgcctt accagggtcg cattctggcg
     1921 ctgcaccatc tgcactggat tgtaggggta atggggtata aggagagaga gaccgcggag
     1981 aggcttgcgg ccggactgat cgccaacctg ggcgagtgat gaaggtattg atcgtcaaca
     2041 ccctctgggg tggtcatccg ggcggcatcg accgctacat atcccaaatc atccacatgc
     2101 tccaccgcgc cggcgagcgt acggcgatgg tctatggccg ccgccggggc gagcccatgg
     2161 agcggaacct ccccctcgag ggcgaagaag ccttgccggg gctggaaagg gctccggagg
     2221 aaaaaaataa ccgggttgcc gaaaggctgc ttacgctggc cgccggaatg cagcccgatg
     2281 tcgtctttgt gcaggacatc gcccacgcac ccgccctctc cgccctgtgc cgctctttcc
     2341 ccgtcgtgag catgttccat gattaccgac ccatctgcct caaggatacc cgccgcgact
     2401 atttcgccag acgcctctgt gaacacccgc ttggctggcg ctgcctgctc aagggccact
     2461 ttatccgtcg gcctgcctcc ggctcacacc tgccccgtct ggccagcctg caggaggcgc
     2521 agcggctcct caatgcctac cagggctgcc atcatgctct caccgccagc gaattcgtcc
     2581 gcagcgccta tctgctcaac ggcttcgccc cccaccgcat cgccaccctg cctctcttca
     2641 ccccggagaa cggttttgat ccggccggct cctatccccg ggaaaagatc ctccttttcg
     2701 tcggccgcct taccgaccgc tacaaggggg cggacctgct cctcgaggtg atggagcgct
     2761 gccgggccga aatccgcctc catatcgtcg gcggcggccg ctacacggcg cggatcaaat
     2821 ccctgtgcgc caaaaaggag cttggccacc gggtcaagtt gatgggctgg atggaggggg
     2881 aggcattgca gcgggcctac cgtcaggcga tggcggtggt gatgccctcg ctctgggcgg
     2941 aacccttcgg gctggtgggg ctggaggcga tggccaacgg caccccggtc gtcgctttcg
     3001 atgtgggggg catcccgcag tggctgcggg agaatgtcac cggatttctt gtcccctggc
     3061 tggacacagc cgcgatggcg gaacgcatcg accggctggc gggaaatccg gtcctggtcc
     3121 ggcaactggg cctcgccgga caccatcatg tgcgcagcga gttttccgag tccgcctatc
     3181 tggagggcct catgagcgcc ttcaggctgg ctatctcccg cttccacgag gagagaggcg
     3241 ggtcactccc ctcctgaatc atccccccgc tgttggtacc atacctctgg ccgcgtctgc
     3301 cagccggtca agggccagaa cagacacggc cgggtaaaga ttgtttcgcc ctctccatac
     3361 ccccccttaa tctcccgagc aagccagtcg tcgttggtga tgatcacctc aaatcgccgc
     3421 cttgccaggg cggtgtcgat ctcgtcaacc agcctcctcc gtccgggttc atcctttccg
     3481 cgcaaaacat catccagggc gcagcggtgc gcacagccgc tcttgccggc catgcggctg
     3541 agataaccgt ggctggggaa ccagacctcc ccctccactg cggcgatgcg gcccacgagc
     3601 gcctcaccgg ccgcccggtc tgcgggggcg ggcagatgat ggcatggatt gtaaagcaga
     3661 agcaggagtt ggagcagggc ggccgcccag ggcagcagag gccgcacagc ccgggcgaac
     3721 cagcgctgaa cggccagagc gaagagtatg gccagagcag cgtgagccgg catggcgacg
     3781 ttgtgaaagg ccccggaggg gacgctcgcc agccagggac cgccgattag ccctgccgct
     3841 gcagccagcc agaagagccg gggcccgtgt aaccggccgg gcccgaggaa aagcgagagc
     3901 cccgcagcaa gggtggcgat gggaaagggc ttgaagaaat agtcgatcca gaaatggtgg
     3961 acccgccacc agaccggcac atgctggccg ggcagataga agaggtagta gaagagccag
     4021 ccatcgcttg ccctttgcag ggtccagagg ccgccgccca ggaggatgag ccaggcggcg
     4081 gggacaagcc atcgccgcgg cccctcgccg aagagaatca gccaaaccat caccggcagg
     4141 gtgatgatca gggcggtctg cttgcagaaa aaagctaggg cgagcaggat ggccgcgccg
     4201 agccccccgg cgagggtgcg gctgcacctc agcaggcaga ggccggccag cagaagcagc
     4261 agaaagagag aatcgacccg cgccaggcca aaccagaaac cgctgaccgg ataactggcc
     4321 agataaagcg tccccgccaa aagcccggcg tgacgggatc cgctctcccg ggcggtccac
     4381 tgcccgatga tgaacacagt gagcaggagg gcgagggtcg atatgccccg cagcagggcc
     4441 aggccggggc cgaagaggcg gccgaggagg acagcgagat ggaaatagag cggcggatag
     4501 ctgaaggggg tgaaatcgag agcgggcggg ccgtagagcc tgccgccctc gagcaggcgg
     4561 tggacatgat cgagcatccc cccctccatc cactccagct cgaagggcca ggggatatga
     4621 cgccaggcca gaaagagaaa tgcagcaagg aaataaaagc cggcgatcca gagggcggcg
     4681 ccggtcaccc ttcctgcaag ggtgctccgg aaaaacaact ctcctcccgc gtcggtatcc
     4741 ggctgttccg gcgcagggtt catggcccgt tccgcctgaa gaggagcaga tttttgtccc
     4801 tgcgcagggg aaaggtgcga acgagctgaa aagagccatc cgcctgcatg tgcgcgtaga
     4861 gggcgcgatg gacaggatcc atcccctcgt cgccgggttc ctgccggtcg ttgagcgcga
     4921 tccagaccgg cctctgcgcc aggataaaat cggcatagcc ggcgaaatcc ccgctcttcc
     4981 agaagcccgg gatctgcttg ttggtgagcc cgatcatgtc gatgacgggc agattggagt
     5041 accagccgat ggcgccgatc tcattgacca ccagtttctc ccccggccgg gcgtgctcgc
     5101 ggagccagag tccgaccgcg cggtggggcc ggc
//
LOCUS       DAOPWU010000259         5113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16363_length_5113_cov_4.682879, whole genome shotgun sequence.
ACCESSION   DAOPWU010000259 DAOPWU010000000
VERSION     DAOPWU010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16363_length_5113_cov_4.682879"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1015
                     /locus_tag="PKM23_11695"
     CDS             29..1015
                     /locus_tag="PKM23_11695"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92176.1"
                     /translation="MKRVIIGIHGLGNKPAADVLHRWWRRSLYEGFRAIGHPRRRLPF
                     ETVYWADILHRIPYDPAIRDRGDARYLEEPYRPSPGGPVPSSRPVRKKILDLVEKQIM
                     RLDPDEGSSLLWKQLNDLVLRRFFVELEAYYTNSLEIAPGAALAYRDIVRTRLANKLR
                     EHSSKEILLIAHSMGSIIAYDVLSSAPPGVQVHTLITIGSPLGMPLIMHNIRLEQKLA
                     PGGKLAAPAPVTHNWYNLADLADKVALNYQLADDFVPNPGGVGVTDIEVFNDYAIDGD
                     PNPHKAYGYLRTPELARLCFNFISAGESPAAIRRNDALFKAWEWGSRLFSPS"
     gene            complement(1021..1899)
                     /gene="argB"
                     /locus_tag="PKM23_11700"
     CDS             complement(1021..1899)
                     /gene="argB"
                     /locus_tag="PKM23_11700"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNY92177.1"
                     /translation="MQKYIEKARVLMEAMPYIQAFRGKTMIVKFGGSIMESETAMREV
                     LQDVVFMKLIGIRPVLIHGGGPAISAGMKARGIPPHFVSGLRVTDSETMELVEDILAH
                     KINAQIVTLLNDLQGEAVGVSGRDHQMIKVDKLMPEVEGEKMDIGRVGRVRYINTDLL
                     REHLNRGRIPVIAPIGVGDDGLSYNINGDLAAGEIASSLGAWKLVYMTDTPGILREQG
                     DEQTLISTINRDQAEELISKGVISGGMIPKVQSCLAALQFGVNKGHIIDGRIRHSLLL
                     EIFTPKGIGTEILKNS"
     gene            complement(1915..3123)
                     /gene="argJ"
                     /locus_tag="PKM23_11705"
     CDS             complement(1915..3123)
                     /gene="argJ"
                     /locus_tag="PKM23_11705"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNY92178.1"
                     /translation="MQFLDNPSITDPRGFTAAAARAGFKRKRRDLCLIVSEVPARCSA
                     KFTTNRVKAAPVLAGIESLRRSGGMVQALVINSGNANAGTGEQGLRDVRDTLAWTSEK
                     SSIPADLILASSTGVIGRYLDMAKMRKAIERAAARLSIAGGAACAEAIMTTDTVEKAF
                     TLRLEIGGAAVTLSGITKGSGMIMPNMATTLAFVTCDAAIDHSLLDGLFTAAVEESYN
                     MITVDGHTSTNDTALIMANGLAGNAEIMPGTAEAELFGEALKTLMLAMARAVVRDGEG
                     ATKFVIIRVGQAASTTAARTIAFAIANSPLVKTALFGQDPNWGRILSAAGMAGPAFDP
                     DRSDLMINHTYIFSQGNPVMLSRREMMALMAPKEIDIELNLFTGGHSAVVYTSDLSYD
                     YVKINAEYHT"
     gene            complement(3196..4212)
                     /gene="argC"
                     /locus_tag="PKM23_11710"
     CDS             complement(3196..4212)
                     /gene="argC"
                     /locus_tag="PKM23_11710"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNY92179.1"
                     /translation="MTRVSIVGASGYTGLELVRILLRHPAVELAHLAVREPAGQRFSD
                     LFPALRGLCDLPLSGLEVEALAQGSDLIFFGLPHTSSMEWVPRFMQRGIKLIDLSADY
                     RLDRADLYRTAYGCEHRQPEALEQFVYGLPEFNRSRIAASAAVANPGCFPTSVELALL
                     PALRRGLIETASIIVDSKTGISGGGRSPKPAFHYPEANENLAAYKVGRHQHEPEMEQE
                     LAKMAGGPVDILFVPHLAPMTRGIFSTIYARLRETADTEEVLALYNEYYRGEPFIRLL
                     PAGEAPQTRHVSGSNFCDIGMAVVGSTLILMSAIDNLVKGASGQAVQDMNIMLGLDET
                     LGLL"
     gene            complement(4205..>5113)
                     /locus_tag="PKM23_11715"
     CDS             complement(4205..>5113)
                     /locus_tag="PKM23_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNY92180.1"
                     /translation="LEALSGDEGLEGGDQVLTALRLYSKERASACTAALQGIIAALEA
                     LIAAQGAVLLPGYTHTRKAMPSSVGMWAGACRDAFAEDLDLLGHAAGLIDQNPLGTGA
                     GYGVPLPLDREMTTAELGFARVLYNPIHAQNSRGKYEGILLHALAVALADINRMASDL
                     ILFTIPGFDYFTLPGVFMTGSSIMPQKKNPDVLELLRARYHEVAACEAQLRAATVNLI
                     SGYHRDLQSTKEALMRGLGTALETLEMAALVVQNLRVNEEACRSAMTDELYATADAYE
                     LVQQGVPFREAYRIIAARFRQEQKND"
BASE COUNT          896 a         1577 c         1669 g          971 t
ORIGIN      
        1 ctatctgaaa agcaaacaga aggaatgaat gaaacgcgtc atcatcggca ttcacggact
       61 gggcaacaag cccgcggcag atgtgctgca ccgctggtgg aggaggagcc tttacgaggg
      121 atttcgcgcc atcggccatc ccagacgccg gctgcccttc gaaacagtct actgggcgga
      181 cattctccat cgcataccct atgatcccgc catccgcgac cgtggggacg cgcgctatct
      241 cgaggagccc taccggccct cgccgggtgg gcctgttccc tcctcccggc cggtgcgcaa
      301 gaagatcctc gatctcgtcg agaaacaaat catgcgtctg gatcccgatg agggatcctc
      361 gctgctctgg aagcagctca acgacctggt gctgcgccgt ttcttcgtcg agctggaggc
      421 ctattacacc aacagcctcg agatcgcccc gggtgctgcg ctggcctatc gggatatcgt
      481 gcggacgcgg ctggcgaaca aactccgcga acacagcagc aaggagatcc ttctcatcgc
      541 ccactccatg ggctcgatca tcgcctacga cgtgctcagc agcgccccgc caggggtgca
      601 ggttcatacc ctgatcacca tcggttctcc cctgggcatg cccctgatca tgcacaatat
      661 ccgcctcgag cagaaactgg ccccgggggg aaagctcgct gcaccggcgc ccgtcaccca
      721 taactggtac aatctcgccg atcttgccga caaggtggcc ctcaactacc agcttgccga
      781 cgatttcgtc cccaatcccg gtggggtggg ggtcaccgac atcgaggtct ttaacgacta
      841 tgccatcgat ggcgatccca atccccacaa ggcctacggc tatctgcgca cccctgagct
      901 ggcgcgcctc tgtttcaatt tcatcagcgc cggggagagt cccgccgcca tccgccgcaa
      961 tgatgccctc ttcaaggcct gggagtgggg gagccgcctt ttcagcccct cctgagtcgc
     1021 ctagctgttt tttagaatct ccgtgccgat gcccttgggg gtaaagattt ccagcaggag
     1081 ggagtggcgg attctgccgt cgatgatgtg gcccttgttg accccgaact gcagggcggc
     1141 caggcaggac tgcaccttgg ggatcatgcc gccactgatg acgcccttgc tgatcagctc
     1201 ctcggcctgg tcacggttga tggtgctgat cagggtctgc tcatccccct gctcgcgcag
     1261 aatgccgggg gtgtcggtca tgtagaccag cttccaggcc ccgagcgagg aggcgatctc
     1321 gcccgccgcc aggtcgccgt tgatattgta gctcaggccg tcatccccga ccccgatcgg
     1381 ggcgattacc ggtatcctgc cccggttgag gtgctcccgc agcagatccg tgttgatata
     1441 acgcacccgg ccgacccggc cgatgtccat cttttcgccc tccacctcgg gcatcagctt
     1501 gtcgaccttg atcatctggt ggtcgcggcc cgagaccccg acagcttccc cctgcaaatc
     1561 gttgagcagg gtgacgatct gggcgttgat cttgtgggcg aggatatcct ccaccagctc
     1621 catggtctcg ctgtcagtga cgcgcaggcc gctgacgaaa tgcgggggga tgccccgcgc
     1681 tttcatgccg gcgctgattg ccggcccgcc gccgtggatg agcacggggc ggatgccgat
     1741 cagcttcata aaaaccacat cctgaagaac ctcccgcata gcggtctcgc tctccatgat
     1801 actccccccg aacttgacga tcatggtctt gccgcggaag gcctgaatgt agggcatggc
     1861 ttccatcaag acccgtgctt tttcgatata cttttgcatg gtgacccctg tccttcaggt
     1921 gtggtactct gcgttgatct tgacatagtc gtaggagaga tccgaggtgt agaccacggc
     1981 ggagtggccg cccgtgaaga gattcagttc gatgtcgatc tccttcgggg ccatcagggc
     2041 catcatctca cggcgggaga gcatcaccgg gttgccctgg gagaagatat aggtgtggtt
     2101 gatcatgaga tcgctgcggt ccggatcaaa ggcgggcccg gccatgcccg cggccgaaag
     2161 gatgcggccc cagttggggt cctgaccaaa gagggcggtc ttgaccaggg gtgagttggc
     2221 gatcgcgaag gcgatggtcc tggccgcggt cgtgctcgcg gcctggccga cccggatgat
     2281 gacgaatttg gtcgccccct cgccgtcgcg caccaccgcc ctggccatgg ccagcatcag
     2341 ggtcttgaga gcctcgccga acagctccgc ttccgctgtg ccgggcatga tttccgcatt
     2401 gccagccagg ccgttggcca tgatcagggc ggtgtcgttg gtgctggtgt ggccgtcgac
     2461 ggtgatcatg ttgtagctct cctccaccgc cgcggtgaag agaccgtcga gcaggctgtg
     2521 gtcgatggcc gcgtcgcagg tgacgaaagc aagggtggtg gccatgttgg gcatgatcat
     2581 cccggagccc ttggtgatgc cgctcagggt caccgccgcg ccgccgatct cgaggcgcag
     2641 ggtgaaggcc ttttcgacgg tatcggtggt catgatcgcc tcggcgcagg cagcgccgcc
     2701 ggcgatgctg aggcgggcgg ccgcccgctc gatcgccttg cgcattttgg ccatgtccag
     2761 atagcggccg atcaccccgg tgctggaggc gaggatgagg tcggcgggga tggagctctt
     2821 ttcggacgtc caggcgaggg tgtcgcggac atcgcgcagc ccctgctcgc cggtgccggc
     2881 gttggcattg ccggaattga tcaccagggc ctggaccatt ccccccgacc tgcgcaggga
     2941 ttcaatgccg gcgaggacgg gtgcggcttt gacccggttg gtggtgaatt tggccgaaca
     3001 gcgggcgggc acctcgctga cgatcaggca gaggtcacgg cgtttgcgtt taaagccggc
     3061 gcgggcggca gcggcggtga agcccctggg gtcggtgatg gagggattgt ccagaaactg
     3121 cattgcattt ttccttttct tgagatgtgg tctccccggc gcggactcgc acgctgggct
     3181 cctgaggctt ccaggctaga gcagccccag ggtttcatcc agccccagca tgatgttcat
     3241 atcctgcacc gcctggcccg aggccccctt gaccagattg tcgatggcgc tcatcaggat
     3301 cagggtactt cccaccacgg ccatgccgat gtcgcagaaa ttggagccgc taacatgacg
     3361 ggtttggggc gcctcgccgg cggggagaag ccggataaag ggctcaccgc gatagtactc
     3421 gttatagagg gctaacacct cttcggtatc cgccgtttcg cgcaatcgcg catagatggt
     3481 gctgaagatg ccgcgagtca tcggcgccag atgagggacg aagaggatat cgaccgggcc
     3541 gccggccatt ttggccagct cctgctccat ttcgggctca tgctggtgcc tgcccacctt
     3601 gtatgcagcc aggttttcat tggcctcggg gtaatgaaag gcgggtttgg ggctcctgcc
     3661 cccgcccgag atcccggtct tgctgtcgac gatgatcgat gcggtctcga tcaaaccccg
     3721 ccgcagggcg ggcagcaggg ccagctcgac gctggtcggg aagcagcccg gattggcgac
     3781 ggcggcggag gcggcgatgc gggagcggtt gaactcgggc aaaccgtaga caaactgctc
     3841 cagggcttca ggttggcggt gttcgcagcc gtaggctgtg cggtaaagat cggcccggtc
     3901 gagccggtag tcggcgctga gatcgatcag cttgattccg cgctgcatga aacggggcac
     3961 ccattccatg ctgctggtat ggggcagccc gaaaaaaatg aggtcgctgc cctgggcgag
     4021 agcctccacc tcaagtccgc tcaggggcag gtcgcagagg ccgcgcaggg cgggaaagag
     4081 gtcggagaag cgctgcccgg cgggctcgcg cacggccagg tgggccagct caacggcggg
     4141 atggcgaagg aggatccgga ccagttccaa cccggtatag ccggaggcgc cgacgatcga
     4201 aaccctagtc atttttctgc tcctgccgga accgggcggc gatgatccgg taggcctcac
     4261 gaaagggcac accctgctgg accagttcat aggcgtctgc ggtggcgtaa agttcatcgg
     4321 tcatggcgga gcggcaggcc tcctcgttga ccctgaggtt ttgcaccacc agggcggcca
     4381 tctcgagagt ttcgagagcc gtgcccagcc cgcgcatgag cgcctccttg gtggactgaa
     4441 ggtcgcggtg gtaaccggag atcagattga cggtcgcggc gcggagctgg gcctcgcagg
     4501 cggcgacctc gtggtagcgg gcgcgcagca gctcgagcac atccggattc tttttttgcg
     4561 gcatgatcga ggagccggtc ataaagaccc cgggcagcgt aaagtaatcg aatcccggta
     4621 tggtgaagag gatgaggtct gaggccatgc ggttgatatc ggccaaagcc accgccaggg
     4681 catgcagcag gatcccttca tacttgccgc ggctgttctg ggcgtgaatg ggattgtaca
     4741 acaccctggc aaagcccagc tccgcggtcg tcatctcccg gtcgagggga agcgggacgc
     4801 cgtagcccgc cccggtgccg aggggattct ggtcgatcaa cccggcggcg tggccgagca
     4861 ggtcaagatc ctcggcgaaa gcgtcgcgac aggccccggc ccacatcccc acgctcgagg
     4921 gcatggcctt gcgggtgtgg gtataaccgg gcaaaagcac agcgccctga gcggcgatga
     4981 gggcctcgag ggcggcgatg attccctgca aggcggccgt gcaggccgag gcccgctcct
     5041 tgctgtagag gcgcaatgcg gtgaggacct gatcgccgcc ctcgaggccc tcatcgccgc
     5101 tcagggcctc gag
//
LOCUS       DAOPWU010000260         5081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16503_length_5081_cov_4.728412, whole genome shotgun sequence.
ACCESSION   DAOPWU010000260 DAOPWU010000000
VERSION     DAOPWU010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16503_length_5081_cov_4.728412"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKM23_11720"
     CDS             complement(<1..605)
                     /locus_tag="PKM23_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HNY92181.1"
                     /translation="MQVIADVVAALDSFLWGLPLILLLFGTHLFLTIRLRFPQRHIFK
                     AIKLSFSRDAGAEGDVSQFGALTTALAATIGTGNIVGVATAVTLGGPGAVLWLWLTGV
                     FGISTKYAEGLLAVKYRIKTPSGDMAGGPMYVLERGLGWRWMGILFAAFTAVAAFGIG
                     CMVQANSISALALSAFQIPGWVTGLLLTILTAVVILGGIKS"
     gene            complement(783..2996)
                     /locus_tag="PKM23_11725"
     CDS             complement(783..2996)
                     /locus_tag="PKM23_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNY92182.1"
                     /translation="MIISARISAPDIVDVQLQDSTAHIDTADFYFTPDLQIYRVEQRK
                     GVFRLHCAPLDLTQTRFLHLRESRAELDPGLLLDRYYSNKTMGCSWDEDSTTFRLFSP
                     RARRVTLTLYPAPEAEAGAGHELSRDQDGVWELTLPGHYFGQAYTYQVDGPAGATEQF
                     DYSKPICDPYARAVVTANEYLHRGRTLILDSGRYDWEGDHPLGISLQEMIIYECHVRD
                     LTAHSSSGCPAELAGSYKGAIQPGLAGGIDYIRALGVNAIELLPVHEFGNIELPFNVD
                     AGGTRNTWNPYSRNHWGYMTSFFFAPESYYASGAAMTPGAMTGGDGRQVDELKDLVKA
                     FHKAGIAVILDVVYNHVSQYDQNCFKLADKKYYFRLDAEQNYIATSGCGNDFKTERPM
                     AQRLILESVRYWMEEYHIDGFRFDLAAMIDWETVDLITREARKINPEVVLIAEPWGGG
                     KYDPAGFSQHDWAAWNDQFRNGIKGQNPENGQSYIFGRYLGDLNREAVKRFIRGTLAA
                     EGGLFQKSSHAVNYLGSHDDHTYGDFVRIGSGGVLPDQVISDPLANARLTTEQLKISK
                     LGALILFTSQGAVMIEEGHEFARSKVIARSEAPDSMAGRIDHNSYNKDNETNWLNYSH
                     AALNQELIDYYKGLIRLRKRYAAFRSTPSGAIHFSDGDNPFALSFQIRRADNPDRRDF
                     LVLLNGHREEMSRFILPEGQWRILVNGEKAGPDPEERRVEGELVLPSTTGMVLVR"
     gene            complement(3149..4627)
                     /locus_tag="PKM23_11730"
     CDS             complement(3149..4627)
                     /locus_tag="PKM23_11730"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNY92183.1"
                     /translation="MDLQLYLDDLGEKKVTGSPAGIDITGIAYDPLRIQPGFIYVAIN
                     IYTQLDKIEIPDGHPYVQEAIARGAVCVVLQNDMELPSGVVKVVVPDSRYALGILANR
                     FYGRPSEQLKLVGVTGTNGKTTTTHVIESIFIENHKVGLIGTLYYKLNGEIRKSKDTT
                     PEPPDLQAILREMADLDFSYCFMETSSHGIEFHRLQGCRFRVALFTNLTQDHLDYHKT
                     MENYLNAKLKLFRWLGEEDFAVINIDDPYGGRFVEATRANVLTYGIYSPADIMARDIH
                     YDINGTRYTLVTPKGEIEIKTRLLGRFNVYNALAGVGTAVAEGIGLEQIKRGLERPIR
                     VAGRMEMVDRGQPFTVVVDYAHTPDGMENVLGLARGLNPRKLITVFGCGGDRDKEKRP
                     IMGGVAARFSDEIILTADNPRNEDPLQILAEIRKGCDSASVTEIVDRRSAIQHVIAMA
                     GPGDIVMILGKGHETTQTLKEHTVPFNDREEAEKALDALLQK"
     gene            complement(4694..>5081)
                     /locus_tag="PKM23_11735"
     CDS             complement(4694..>5081)
                     /locus_tag="PKM23_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNY92184.1"
                     /translation="TLASSLAQTRTAYQRRLAEAEELHRIVSRVEAPVILAGDFNATP
                     YSPLYRRFSDELQNSFATAGWGLGTTYGHYSLQGSVSKYLRFLIFDFLRIDQVFASRH
                     FRILEARVMPLSISDHRPQLVRLVLY"
BASE COUNT          957 a         1427 c         1722 g          975 t
ORIGIN      
        1 atgctcttga tgcccccgag gatcaccacc gcggtgagga tggtgagaag aaggccggtc
       61 acccagccgg ggatctggaa ggcgctcagg gcgagggcgg agatcgagtt ggcctgcacc
      121 atgcagccga taccaaaggc ggcaacggcg gtgaatgcgg caaagaggat gcccatccag
      181 cgccagccca gtccgcgttc gagcacatac atcggccccc ctgccatatc cccgctcggg
      241 gtcttgatgc ggtatttgac cgccagcagc ccctcggcgt acttggtgct gatgccgaaa
      301 acccccgtca gccagagcca caacaccgcc ccgggcccgc caagggtgac cgcggtcgcc
      361 accccgacga tattgccggt gccgatggtc gcggctaggg ctgtggtcag ggcgccgaac
      421 tggctgacat ccccctccgc gcctgcatcc cgggagaagg agagtttgat tgccttgaaa
      481 atgtggcgct gggggaagcg cagccggatg gtgagaaaaa gatgggtgcc gaaaaggagg
      541 agtatcaacg gcaggcccca gagaaaactg tccagagcgg cgacgacatc ggctatcacc
      601 tgcatgggta cctcacggat ggagttagga taggctgcat gctctgtact ttcttctgcg
      661 cgcccaggcc agccgagcgc agcacgcggt cacccctggc cgggaagcct cccggcggcg
      721 gaagcagcga tggcaacaat gaagcagcct gaaccgccgg gatgagggct ttcacacagc
      781 cgtcaccgga ccaggaccat gccggtggtc gaggggagga ccagctcgcc ctcaacccgc
      841 cgttcctcgg gatcggggcc ggccttctcg ccgttgacca ggatgcgcca ctggccctcg
      901 ggcaggatga aacgcgacat ctcctcgcgg tggccgttga gaagaacaag gaaatcgcgg
      961 cggtcgggat tatccgcccg cctgatctga aaagagaggg cgaagggatt gtcgccgtcg
     1021 ctgaaatgga tcgccccgga cggagtgctg cgaaaggccg catagcgctt gcgcagcctg
     1081 atcagcccct tgtaataatc gatcagctcc tggttgaggg ccgcgtggct gtaattgagc
     1141 cagttggtct cgttgtcctt gttgtagctg ttgtgatcga tgcggccggc catcgaatcc
     1201 ggggcctcgc tgcgggcgat caccttgctg cgggcgaatt cgtgcccctc ctcgatcatc
     1261 accgcgccct ggctcgtgaa aaggatgagg gcgccgagct tgctgatctt gagctgctca
     1321 gtggtcaacc gggcgttggc gaggggatcg gagatgacct ggtcgggcag aacgcccccc
     1381 gagccgatgc ggacgaaatc cccgtaggtg tggtcgtcgt gggaaccgag gtagttaacc
     1441 gcatggctgc tcttctggaa gagccccccc tccgccgcga gcgtgccgcg gatgaagcgc
     1501 ttgacggcct cacggttgag gtcccccaga tagcggccga agatgtagct ctgaccgttc
     1561 tccggattct gccccttgat gccgttgcgg aactggtcgt tccaggccgc ccagtcgtgc
     1621 tgcgaaaagc cggccgggtc gtatttaccg ccgccccagg gttcggcgat gagcacgacc
     1681 tcggggttga tcttgcgcgc ctcgcgggtg atgagatcga ccgtttccca gtcgatcatg
     1741 gccgccagat cgaaacggaa gccgtcgata tgatactcct ccatccagta gcggacgctc
     1801 tcgaggatta gccgctgcgc catcgggcgc tcggtcttga aatcattgcc acagccgctg
     1861 gtcgcgatat agttctgctc cgcatcgagg cggaaataat actttttatc cgccagttta
     1921 aagcagttct gatcgtattg ggagacatgg ttatagacca cgtcgaggat gacggcgatg
     1981 ccggccttgt gaaaggcctt gaccagatcc ttgagttcgt cgacctggcg gccatccccg
     2041 ccggtcatgg ccccgggggt catggctgcg ccgctggcgt aataggactc gggagcgaag
     2101 aaaaaggagg tcatgtagcc ccagtggttg cggctgtagg gattccaggt gttgcgggtg
     2161 cccccggcat cgacattaaa gggaagctcg atattgccga attcatggac cggcaggagt
     2221 tcgatggcgt tgacacccag cgcgcggata taatcgatgc cgccggccag gccgggctgg
     2281 atcgccccct tgtagctgcc ggccagttcc gccggacagc cagaactcga atgggcggtc
     2341 agatcgcgga catggcactc gtaaataatc atctcctgca gggaaatgcc caggggatgg
     2401 tccccctccc agtcgtagcg gccgctgtcg aggatgaggg tgcggccgcg atggaggtac
     2461 tcgttggcgg tgaccacggc ccgcgcatag ggatcgcaaa tgggcttgct gtaatcgaac
     2521 tgctcggttg cccccgcggg gccgtcaacc tggtaggtgt aggcctggcc gaagtaatgg
     2581 ccgggcaggg tgagctccca gaccccgtcc tgatcgcgac tgagctcgtg gccggcaccc
     2641 gcctccgcct ccggggccgg atagagcgtc agggtgaccc ggcgggcgcg cggcgaaaaa
     2701 aggcggaagg tggtcgaatc ctcgtcccag ctgcagccca tggtcttgtt cgagtagtag
     2761 cggtcgagga gaaggccggg atccagctcc gccctgcttt cgcggaggtg gagaaagcgg
     2821 gtctgggtca gatcgagggg agcacagtga agacggaaga cgcccttgcg ctgttcgacg
     2881 cggtagattt gcagatcggg ggtgaaatag aaatcggcgg tatcgatgtg ggcggttgaa
     2941 tcctgcagct gcacatcgac gatgtccggg gctgatatcc tggcggagat gatcataggc
     3001 gatcttttat ggcatcctga tatgagagta acgcccacgc agaggaggac gagggtgctc
     3061 aggaacagca cctccaccct gacgcccttg cacaggcttt tgcatgcagt gctcatggga
     3121 gtcatccttc gttttgggat gaggatgatt atttttgcag cagcgcatcg agcgcctttt
     3181 cagcttcttc acggtcgttg aaggggacgg tgtgctcctt gagggtctgg gtggtctcat
     3241 ggcccttgcc gaggatcatg acaatgtcgc ccgggccggc catggcgatg acatgctgaa
     3301 tggcggagcg gcggtcgaca atctcggtaa cgctggccga gtcgcagccc ttgcggatct
     3361 cggcgagaat ctggagggga tcctcattgc gcggattatc cgccgtgagg atgatttcgt
     3421 cgctgaagcg ggcggcgaca ccccccatga tcggccgctt ctccttgtcg cggtcgccgc
     3481 cgcagccgaa aacggtgatg agcttgcgcg gattgagccc gcgcgccagg ccgagcacat
     3541 tctccatgcc gtcgggggtg tgggcgtaat cgacgaccac ggtgaagggc tggccccggt
     3601 cgaccatctc catgcggccg gcgacgcgga tcgggcgttc cagcccgcgc ttgatctgct
     3661 cgaggccgat gccctcggcg acagccgtgc cgaccccagc cagggcgttg tagacgttga
     3721 agcggccgag cagtcgggtt ttgatttcga tctccccctt cggggtcacc agggtatagc
     3781 gggtgccgtt gatatcatag tggatgtccc tggccatgat gtcggccggg gagtagatgc
     3841 cgtaggtgag gacattggcg cgggtggcct cgacgaagcg gccgccgtag ggatcatcga
     3901 tgttgatgac cgcgaaatcc tcctcgccga gccagcggaa gagcttgagc ttggcgttga
     3961 gatagttctc catggtcttg tgataatcga gatgatcctg ggtgaggttg gtgaagaggg
     4021 cgacccggaa gcggcagccc tgaaggcggt ggaactcgat gccgtgcgag gaggtctcca
     4081 tgaagcagta cgaaaaatcc aggtccgcca tctcgcggag gatggcctga aggtccggcg
     4141 gctcgggggt ggtatccttg gatttgcgaa tttcgccgtt gagcttgtag tagagggtgc
     4201 caatgaggcc gaccttgtgg ttctcgataa agatggattc gatgacatgg gtcgtggtgg
     4261 tcttgccgtt ggtgcccgtc accccgacca gcttgagctg ctcggaggga cggccgtaaa
     4321 agcggttggc gaggatcccg agggcgtagc gggagtcggg caccaccacc ttgacgacgc
     4381 cggaggggag ttccatgtcg ttttgcagca cgacgcagac tgcgcccctg gcaatggctt
     4441 cctggacata gggatgtccg tcggggatct cgattttgtc cagctgggtg tagatattga
     4501 tggccacgta gatgaatccg ggctgtatgc gcaggggatc gtaggcaata cccgtgatgt
     4561 cgattcctgc cggcgagccc gtcacctttt tttcgcccag gtcgtccaga tagagctgca
     4621 aatccatgtt catctcctcg cgagaaagtg ggtggataat agtgcggaaa tgtagctaaa
     4681 aattaggttc aagtcaatac aaaacaaggc gcacgagctg cggccggtgg tccgaaatcg
     4741 agagcggcat cacccttgct tccaggatgc ggaaatggcg gctggcgaag acctgatcga
     4801 tgcgcaggaa atcgaagatc aggaagcgca ggtatttcga gacactgccc tgcaggctgt
     4861 agtggccgta ggtggtgccc agcccccagc ctgcggtcgc gaagctgttc tgcagttcgt
     4921 cgctgaaacg gcggtagagg ggactgtagg gggtggcgtt gaaatccccg gcgagaatga
     4981 cgggcgcctc gacgcggctg acgatgcggt gcagctcctc cgcttccgcc aggcgccgct
     5041 gataggcggt gcgtgtttgg gccagggaac tggcgagggt g
//
LOCUS       DAOPWU010000261         5070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16557_length_5070_cov_4.756929, whole genome shotgun sequence.
ACCESSION   DAOPWU010000261 DAOPWU010000000
VERSION     DAOPWU010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16557_length_5070_cov_4.756929"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PKM23_11740"
     CDS             <1..952
                     /locus_tag="PKM23_11740"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNY92185.1"
                     /translation="LTEQNYREARAAAEHLIGLTEGMLKKYPERIRLAATPAEIRQNE
                     RDGVLSACVGIENGFALGHDLTALQAFRARGVRYVTLCHTRDNDICDSSTDPGADSWG
                     GLSPFGRQVIAEMNRLGILVDLSHASDEAFFQALAASKTPIFASHSCARALCNHPRNL
                     SDEMLKALAATGGVMQMCILSEYVKAIPANARREAAQDSLDAIYGTWESLREPVARKA
                     KREAWNEVDRLYPRTLATVADVCDHIDHVVKLVGIDYIGIGTDFDGGGGVNGCNEVSQ
                     MCNITAELLRRGYSREDLAKIWGGNFLRVFTRALEAAEAE"
     gene            952..2274
                     /locus_tag="PKM23_11745"
     CDS             952..2274
                     /locus_tag="PKM23_11745"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNY92186.1"
                     /translation="MKKIDPNLLAIAFTEHDLCTRCGTCAGACPVGAITLDENFFPVL
                     SADACTECGLCARVCPGGRVNFLDLTELTFGHRNPATRFDGHVVATYVGYAGDKQLRR
                     GGAGGGVITALMWDLLQRGLVDGCIVSRIKPQQPWLGEVFIARSREELRASQQSKYII
                     IPVNEILAAVRDTPGRFAFAGLPCQVHGLRLLCAESPALKEKIRVVIGLFCASSLEPY
                     VAREMLTCRGIKPDQIRGFEFRGGAWPGKIRATLRDGRIKQLHYSNFKDGAINYLTYL
                     FSPPRCQTCIDGAAEFADISVSDAWTRDEFGNYCFESHSKLLARTPRGVEVMKEAIAS
                     GALVASDVTANRAYQTHKLHTQKKGLNSPLRVGRLRGAGKIAPLYDRPAPEASAREVL
                     AERMESAIMALGRVRSIRLPLFKFLTSRYGILFVKIRQWRKSRKYRRK"
     gene            2300..4144
                     /locus_tag="PKM23_11750"
     CDS             2300..4144
                     /locus_tag="PKM23_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNY92187.1"
                     /translation="MKSPLRRLIPVALWLLLFSPLSPPAAGQTGQEEGQPLKELRFEG
                     NDHFSDGELREQLFLVARTGLARLQFWRKGPVYRESTVQRDLRGLLRFYQREGFIHAR
                     IEAASSFISPSAVRLTYQINEGTRVAIASAQVRLADSSRAVVEVWEKARVKMITQSGS
                     GYSDAAVQNDMRSLNTLFANQGYAYAETRVQPRLEDQDRIVQLLYEVDPGPRCLFGPV
                     TILGDSIISPRTIEKQIALRPGRPYSQKNLEKSQRQIYQLGVFQYVTVKAMLDSMENP
                     LLPVVIQVKAAPPWTIKTGIGYGMEERLRLLVDIRKRSFLGGARRLDLYLKHSSLEPY
                     SIDLRITQFGFPAPQTSLLLNPFFLRQQEPGFTVDRTGVNLAHQQRFATYTDGSLRYT
                     LEQDYLKVSALTREQTLDSSRIALYHKSYFTLAMARDNSLPLFYPRKGLYTAATVTWA
                     GVGFQSDFHFLRLLGEVRHYQRVGRTSVLATRIKAGSLRRLGPDRFTPIEERFFTGGA
                     SSVRGWGHSEIGPKNSAGLPIGGNSLLELGVELRREWAGPISGALFFDAGNVWPRLHG
                     HNLGDLCTAAGAGLRYRTPIGPIRLDFGWPLNRSGKGVQFHLSVGQAF"
     gene            4154..>5070
                     /locus_tag="PKM23_11755"
     CDS             4154..>5070
                     /locus_tag="PKM23_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92188.1"
                     /translation="MRRFLKITAILLMLALAAGLVALGILYQSGDLHRRAREAVVKLA
                     TGAFDGEIEFGRISGSLLQTLQIEDLRLFRGADTLLQVDTLRCRWRPISLLWGHLTLH
                     RVEFIGTRIAVHQAGDGSWNYERYLRNAGEPSGREWTLQLKSLRLRDTAVRVQPAGDS
                     PAPVVLRIPNLAAGARLSPAGWRIGLEEAEVSLPSYGIRITRVQAKAAGNERGLVLDL
                     SRLETTASRIKGSLEAAFGPGAPVTITLHADSLAAADIAPLTGWSPPFARMGIDIRAA
                     GYRDSIAVEVKAGAPEGNAELKGWLKPAEA"
BASE COUNT          896 a         1824 c         1465 g          885 t
ORIGIN      
        1 cctgaccgaa cagaactacc gagaagcccg cgccgctgcc gaacatctga tcgggctgac
       61 cgagggcatg ctgaaaaaat accccgaacg catccgcctt gccgccaccc ctgccgagat
      121 ccgccagaac gagcgcgacg gcgtactcag cgcttgcgtc ggcatcgaaa acggcttcgc
      181 cctcggccac gacctgaccg ccctgcaggc cttccgcgcg cgcggggtgc gctacgtcac
      241 cctctgccac acccgcgaca acgatatctg cgactcctct accgatccgg gcgccgatag
      301 ctggggagga ctcagtccct tcggacgcca ggtcatcgcc gagatgaacc ggctcggcat
      361 tctggtcgac ctctcgcacg cctccgacga ggcctttttt caggccctcg cggcatcgaa
      421 aaccccgatc ttcgccagcc attcctgcgc ccgcgccctc tgcaaccatc cccgcaacct
      481 cagcgatgag atgctcaagg ccctcgccgc cacgggaggg gtgatgcaga tgtgcatcct
      541 cagcgaatat gtcaaggcca tccccgccaa cgcccgccgc gaggcggcgc aggattccct
      601 cgacgccatc tatggaacct gggaatccct cagggaaccg gttgctcgca aggccaagcg
      661 cgaggcctgg aacgaggtcg accgcttata tccgcgcacc cttgcgacgg tcgctgatgt
      721 ctgcgaccat atcgaccatg tcgtcaagct ggtggggatt gactatatcg gcatcggcac
      781 cgactttgac ggcggcgggg gggtgaatgg ttgcaacgag gtctcgcaaa tgtgcaatat
      841 cactgccgag ctgctgcggc gcggctacag cagggaagac ctggccaaaa tatggggggg
      901 caacttttta cgcgtcttta cccgcgccct ggaggccgct gaagcagagt aatgaaaaaa
      961 atcgatccca acctcctcgc catcgccttc accgagcacg acctctgcac ccgctgcggc
     1021 acctgcgccg gggcctgccc ggtcggagcg atcaccctgg atgaaaactt tttcccggtg
     1081 ctcagtgccg acgcctgcac cgagtgcgga ctctgcgccc gcgtctgccc cggcggcagg
     1141 gtcaacttcc tcgacctcac cgaactcact ttcggccacc gcaatcctgc gacccgcttc
     1201 gacggccatg tcgtggccac ctacgtcggt tatgccggcg ataaacagct gcgccgcggc
     1261 ggcgccggcg gcggggtgat caccgccctg atgtgggacc tgctccagcg cggcctcgtc
     1321 gacggctgca tcgtctcccg catcaaaccg cagcagccct ggctcggcga ggtgtttatt
     1381 gcgcgctccc gggaggagct gcgcgccagc cagcagtcca agtatatcat cattccggtc
     1441 aacgaaatcc tcgctgcggt gcgcgacacc ccgggccggt tcgcctttgc cgggctgccc
     1501 tgccaggtgc acggcttgcg cctgctctgc gccgaatccc ccgccctcaa ggagaagatc
     1561 cgggtggtca tcggcctatt ttgcgcctcc tccctcgagc cctatgtcgc acgcgaaatg
     1621 ctgacctgcc gcggcatcaa acccgatcag atccgcggct tcgagttccg cggcggggcc
     1681 tggccaggga agatccgcgc caccctgcgc gacggacgga tcaaacagct ccactattcc
     1741 aatttcaagg acggggcgat caactacctc acctatctct tcagcccgcc gcgctgccag
     1801 acctgcatcg acggcgccgc cgagttcgcc gatatttcgg tcagcgacgc ctggacacgg
     1861 gacgagttcg gcaactactg cttcgaatcc cattccaagc tgctggcacg caccccgcgt
     1921 ggggtcgagg tgatgaaaga ggccatcgcc tcaggagccc tggtggccag cgatgtgacc
     1981 gccaaccgcg cctaccagac ccacaagctg cacacccaga agaaggggct gaactcaccc
     2041 ctgcgggtgg gccgcctgcg cggggccggc aagattgccc ccctctacga ccggcccgcg
     2101 cccgaggctt ccgcccgcga ggttctcgcc gaacggatgg agtctgccat catggccctg
     2161 ggacgcgtgc gctccatccg cctccccctc ttcaagtttc tcacatcgcg ctacggcatc
     2221 ctctttgtaa agatcaggca atggagaaaa agccggaaat accgccgcaa atagcggccc
     2281 atgaccgggg aggccagctg tgaaatcccc tctccgccgg ctcattccgg tggccctctg
     2341 gttattgctc ttctcccccc tctcccctcc cgcggccggg cagaccggcc aggaggaggg
     2401 gcagcccctc aaggaactcc gcttcgaggg caatgaccac ttttcggatg gcgaactgcg
     2461 cgaacagctc ttcctggtcg cacgcaccgg cctggcccgc ctccagttct ggcgcaaggg
     2521 cccggtttac cgcgaaagca cggtgcagcg ggacctgcgc gggctgctgc gtttttatca
     2581 aagggagggg ttcatccatg cccgcatcga ggccgccagc tcgttcattt ccccctccgc
     2641 cgtgcgcctc acctaccaga tcaatgaggg gacacgggtc gccatcgcct ccgcccaggt
     2701 ccgcctggcc gattcgagcc gggcagtggt ggaggtctgg gagaaggcga gggtgaagat
     2761 gatcacccag agcggctcgg gctatagcga cgcggccgtc cagaacgata tgcggagcct
     2821 caacaccctc ttcgccaacc agggctacgc ctatgccgag acacgggtgc agccccggct
     2881 ggaggatcag gaccgcatcg tccagctcct ttatgaggtc gatcccggcc cccgctgcct
     2941 cttcggcccg gtcaccatcc ttggagacag catcatcagc ccgcgcacca ttgaaaaaca
     3001 gatcgccctc cgacccggtc ggccctacag ccagaaaaac cttgaaaaaa gccaacgcca
     3061 gatctaccag ctcggggtat ttcagtatgt caccgtcaag gcgatgctcg attccatgga
     3121 aaatcccctc ctccccgtcg tcattcaggt caaggccgcc ccgccctgga ccatcaagac
     3181 cggcatcggc tacggcatgg aggagcggct gcgcctgctg gttgatatcc gcaagcgcag
     3241 cttcctcggc ggcgcccgca ggctggacct ctatctgaag cacagcagcc tcgaacccta
     3301 ttcaatcgat ctcagaatca cccagtttgg ctttccggct ccccagacca gcctgctcct
     3361 caaccccttc ttcctgcgtc agcaggagcc aggcttcacc gtcgaccgca cgggagtcaa
     3421 cctcgcccat caacagcgct tcgccaccta taccgacggc tctctgcgct ataccctcga
     3481 gcaggattat ctcaaggtga gcgccctgac ccgggagcag accctcgaca gcagccgcat
     3541 cgccctctat cacaaatcct atttcaccct tgccatggcg cgggacaact ccctccccct
     3601 cttctatccg cgcaaggggc tctataccgc cgccaccgtc acctgggccg gagttggatt
     3661 ccagagcgat ttccattttc tcaggctgct cggggaggtg cggcactatc agcgtgtggg
     3721 ccgcaccagc gtcctggcga cgcggataaa ggctggttcg ctgcgccggc tcgggccgga
     3781 ccgcttcacc cccatcgagg agcgtttttt caccggcggc gcctcctcgg tgcgaggctg
     3841 gggccacagc gagatcggtc ccaagaacag cgccggcctt cccatcgggg gcaacagcct
     3901 gcttgaactg ggggtcgaac tgcgccggga gtgggccggg cccatctccg gcgccctctt
     3961 tttcgatgcg ggcaatgtct ggccgcggct gcacggccac aacctcgggg atctctgcac
     4021 cgccgcaggc gccgggctgc gctatcgcac gcccatcgga ccgatccggc tcgatttcgg
     4081 ttggcccctc aatcgcagcg gcaagggcgt ccagttccat ctcagcgtcg gtcaggcctt
     4141 ttaggaaaat gtcatgcgcc gtttcctcaa aataaccgca atcctgctca tgctggccct
     4201 ggccgccggc ctcgttgccc tgggcatcct ctatcagagc ggcgacctcc accgccgcgc
     4261 ccgggaggcc gttgttaaac tggccaccgg ggcgttcgac ggtgaaatcg agttcggccg
     4321 catcagcggc tccctgctgc aaaccctgca gatcgaggat ctccgcctct tcaggggggc
     4381 cgataccctc ctgcaggtcg acaccctgcg ctgccgctgg cgccccatca gcctgctctg
     4441 gggtcacctc accctgcacc gcgtcgagtt cattggcacg cgcatcgcag tgcatcaagc
     4501 gggcgacggg agctggaact acgagcgcta tctcaggaat gcgggcgagc cctccggccg
     4561 ggagtggacc ctgcagctca aatccctccg cctccgcgac accgccgtgc gggtgcagcc
     4621 cgccggcgac agccccgccc cggttgtcct ccgcatcccg aatctcgccg ccggcgcccg
     4681 cctctcgccc gcaggttggc ggattgggct cgaagaggcg gaggtctctc tcccctctta
     4741 cggtatcagg atcacccgag tgcaggctaa agctgcgggc aacgagcgcg gccttgtttt
     4801 ggacctctcg cgccttgaaa ccaccgcctc ccgcatcaag ggcagccttg aggccgcatt
     4861 cggacccggc gcccccgtga ccatcaccct gcacgctgat tctctcgccg ccgctgatat
     4921 cgccccactg accggatggt cacccccctt cgccaggatg gggattgaca tacgcgctgc
     4981 gggctatcgc gattccatcg ctgtcgaagt gaaggccggt gctcccgagg ggaacgcgga
     5041 gttgaagggc tggttgaaac cagccgaagc
//
LOCUS       DAOPWU010000262         5062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16600_length_5062_cov_4.599960, whole genome shotgun sequence.
ACCESSION   DAOPWU010000262 DAOPWU010000000
VERSION     DAOPWU010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16600_length_5062_cov_4.599960"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PKM23_11760"
     CDS             <1..206
                     /locus_tag="PKM23_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HNY92189.1"
                     /translation="PLDAATDAVVLGTQVNAVVLVVRAGKTKIGKAKEKMEIFNTVPV
                     KLVGAVLNSSDTQLVQNYSYYHY"
     gene            227..1177
                     /locus_tag="PKM23_11765"
     CDS             227..1177
                     /locus_tag="PKM23_11765"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNY92190.1"
                     /translation="MHLLLTGGAGFIGSHFSRKLLDEGHEVDCLDNFNTYYDPALKRE
                     NVQPFLDHPSYRLIEGDIVDWPAVENLFAERSYDSVVHLAARAGVRPSIREPLLYEQV
                     NVLGTMHILEAARRHGVGRIVSASSSSVYGSNSKVPFSESDPVDHPISPYAATKKAGE
                     LMAYTWHHLYGLSISCMRFFTVYGPRQRPDMAIHKFARLICSGQPVPVFGDGRSRRDY
                     TYIDDIIEGLYAALLRCEGYHIYNLGESNTIELLELIHLLEEGLGRKAVLEFHPDQPG
                     DVPITYADISLARRELDYRPSTPIERGIGLFAEWYKSRHK"
     gene            1252..2709
                     /locus_tag="PKM23_11770"
     CDS             1252..2709
                     /locus_tag="PKM23_11770"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNY92191.1"
                     /translation="MVSEKERFLGNVMRVADVLTLLLTFPLAYFVDEFIRTVAELNVK
                     AYAVSPTFSGFLYFASNYWMMIIGFPIIWAGIFSLYGIYRNYRTRSFRKYTWLLFVST
                     VWATIASGSLIFILKLELASRLYFSVFVILAFALLLLERKLLLLFLDRIHSRGYNREN
                     LLIVGTGKRARDFIRAIKLHSNWGLHIVGLIDDEHGMYGKEVDGYRVIGRIQDIPFIV
                     KRLVIDRVIFVVPRLWLHRIEEAILSCEELGISTSIALDLFNLHIARTRQTDFNGFPL
                     LEFETFHAKEWQLFIKRIMDLVISSVTLILLSPLLLLVAVLIKATSRGPVLFKQTRIG
                     LNGRKFTLYKFRSMVVDAEARKMELMHRNEMDGPVFKIKRDPRITSIGYIIRKTSIDE
                     LPQFINVLMGDMSVVGPRPPLVGEVEAYKVWQRRRLSLKPGITCIWQVSGRNKIGFDK
                     WMKMDLEYIDNWSLWLDIKILFKTMFVVLTGYGAE"
     gene            2723..3943
                     /locus_tag="PKM23_11775"
     CDS             2723..3943
                     /locus_tag="PKM23_11775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY92192.1"
                     /translation="MINYTLPEVGKKLRILMVAPQPWFQPRGTPFSVLHRIKALSLLG
                     HSVDLVTYPIGQDVPMPGLRILRAASVPGIKKVKIGPSGAKIALDAVLYRRARQQAAR
                     GGYDLLHTHEEAGFFGVNLARRYGMPHLYDMHSSLPQQLHNFRFSRSRLLVGAFERLE
                     ARTIHQAAAVITICPELQNYVEEKFPGKTSLLIENVADNALLFPPDEANAARLRQQYE
                     LGSSRIILYYGTLEAYQGIPLLIESAAQLFAGGGMDDVRFLLVGGSPEQVEACKALTR
                     QHGVAERFRFTGFVQPQLIPAFIDLAEVLVSPRLSGTNSPLKIYSYLRSGRVIVATRH
                     ITHTQILNDEVAILADVTAEGFADGMRRALVERPESAARIAAAAALAERAYSYTEYLR
                     RVQWIVAHAAGQPN"
     gene            4030..>5062
                     /gene="asnB"
                     /locus_tag="PKM23_11780"
     CDS             4030..>5062
                     /gene="asnB"
                     /locus_tag="PKM23_11780"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNY92193.1"
                     /translation="MCGICGIYNRESGAAVAGADIKAMCDAIIHRGPDDEGQYIHGPV
                     GLGMRRLAIIDLATGRQPIFNEEGDAVIVLNGEIYNHLELKRDLEKRGHRFRTRSDTE
                     AILHAYEEYGENCPSHLNGMFAFAIWDARRQRLFIARDRIGKKPLYYHFDGRRLVFAS
                     EIKAILRLREIPREVDPRALDAYLTYEYIPAPLSIFKGIRKLPQGHWLSLDREGLRIR
                     RYWQLHYRASAASEADLAAEFLDLLRDAVRIRLMSEVPLGAFLSGGLDSSAVVAMMSQ
                     TAEKGVKSYSIGFDNATYNELPWARMVARHFGTEHHEEIITPDAVGLTERIVRQLDEP
                     LGDFSVFPTW"
BASE COUNT          907 a         1652 c         1494 g         1009 t
ORIGIN      
        1 cgccgctgga tgcggcgacc gacgccgtcg tgctcggcac ccaggtgaac gccgtcgtgc
       61 tggtggtgcg agcgggcaag accaaaatcg gcaaggccaa ggagaagatg gagatcttta
      121 ataccgtccc tgtcaaactc gtcggcgctg tcctcaacag ctccgacacc cagctggtgc
      181 agaactacag ctattaccat tattaagcga aaggccggac gcgaccatgc atcttctcct
      241 gaccggcggt gccggtttca tcggttccca tttttcccgc aagctgctcg atgaggggca
      301 tgaggtcgac tgtctggaca atttcaacac ctattacgac cccgccctca aacgcgagaa
      361 cgttcagccc tttctcgatc atcccagcta ccggctcatc gagggcgaca tcgtcgactg
      421 gccggcagtg gagaatctct tcgccgagcg gagctatgac agcgtggtcc atctcgccgc
      481 gcgcgccggg gtgcggcctt cgatccggga gcccctgctc tacgagcagg tgaatgtgct
      541 cgggacgatg cacatccttg aggccgcccg ccggcatgga gtgggcagga tcgtctccgc
      601 ctcctcctcg tcggtctacg gcagcaacag caaggtcccc ttttccgaga gcgatccggt
      661 cgatcatccg atctcgccct atgcagcgac caaaaaggcg ggggagctga tggcctacac
      721 ctggcaccat ctctacggcc tttcgatcag ctgcatgcgt tttttcacgg tctacggccc
      781 gcgccagcgg cccgacatgg cgatccacaa attcgcccgg ctcatctgct cggggcagcc
      841 ggtgccggtt ttcggtgacg gccgttcgcg ccgcgactat acctatatcg acgacatcat
      901 tgagggactg tacgccgccc tgctccgctg cgagggctat catatctata acctgggcga
      961 atccaacacc atcgagctgc tcgagctcat ccatctgctc gaagaggggc tcgggcgcaa
     1021 ggccgtgctc gagttccatc ccgaccagcc cggggatgtg cccatcacct atgccgacat
     1081 cagcctggcg cgcagggagc tcgattaccg cccctcgacc ccgattgagc gcggcatcgg
     1141 cctgtttgct gaatggtaca agagccgaca taaatagtgc atccattgcc cggttgtgcg
     1201 gtcagactgc cctgcaggcg ctgacctgtg aattttgatg aagtgagtgg tatggtctct
     1261 gagaaggaaa gattcctggg gaatgtgatg cgggtggcgg atgtcctgac cctgctcttg
     1321 acctttcccc tcgcctattt cgtcgacgag ttcatccgga cggtcgccga actcaacgtc
     1381 aaggcctacg cggtctcccc gaccttttcg ggattcctct atttcgcctc caactactgg
     1441 atgatgatca tcggcttccc catcatctgg gcggggattt tctcccttta cggcatttac
     1501 cgcaattatc gcacccgctc cttccgcaag tatacctggc ttctcttcgt ctcgacggtc
     1561 tgggcgacca tcgcgtccgg aagcctgatc ttcatcctca agctcgaact ggccagccgc
     1621 ctctattttt cagtctttgt cattctcgcc tttgccctcc tgctgctaga gcgcaagctc
     1681 ctgctgcttt tcctcgaccg catccacagc cgcggctaca accgggagaa cctgctcatt
     1741 gtcggcaccg gcaagcgggc gcgcgatttc atccgcgcta tcaagctgca ttccaactgg
     1801 gggctgcaca tcgtcggcct catcgacgac gagcacggca tgtacggcaa ggaggtcgac
     1861 ggctaccgtg tgatcggccg catccaggac atcccattca ttgtcaagcg gctggtgatc
     1921 gaccgggtga tctttgtcgt gccgcggctc tggctccacc gcatcgaaga ggcgatcctc
     1981 tcctgcgagg agctgggcat ctccacctcg atcgccctcg atctcttcaa cctgcacatc
     2041 gcccgcaccc ggcagaccga tttcaacggt tttcccctgc tcgagttcga gacctttcat
     2101 gccaaggagt ggcagctctt catcaagcgg atcatggatc tggtgatctc ctccgtgacg
     2161 ctgatactgc tctcgcctct gctgctgctg gtcgccgtgc tcatcaaggc cacctcgcgg
     2221 ggaccggtac tcttcaagca gactcgcatc ggcctcaacg ggcgcaaatt caccctttat
     2281 aaattccgtt cgatggtggt cgatgccgag gcgcggaaaa tggagctcat gcaccgcaac
     2341 gagatggacg gcccggtctt caagatcaag cgtgatccgc gcatcacctc gatcggatat
     2401 atcatccgca agaccagcat cgacgagctg ccccagttta tcaatgtcct gatgggcgac
     2461 atgagcgtgg tcggtccgcg gccgcctctg gtcggtgagg tcgaggccta caaggtctgg
     2521 cagcggcgcc gcctgagcct caagccgggc atcacctgca tctggcaggt gagcggccgc
     2581 aacaagattg gattcgacaa atggatgaag atggatctgg aatatatcga taactggtcg
     2641 ctctggctgg atatcaagat tctgttcaag acgatgttcg tcgtcctgac cggttacggg
     2701 gctgagtgag gagagtgagg agatgatcaa ctataccctg ccggaggtgg gcaagaagct
     2761 gcgcattctg atggtggcgc cccaaccctg gtttcagcct cgcggcactc ctttcagtgt
     2821 cctgcaccgg atcaaggccc tgagcctgct cggccacagt gtcgatctgg tcacctatcc
     2881 catcggccag gatgtgccga tgcccggtct gcgcatcctc cgcgccgcct ccgtgcccgg
     2941 catcaaaaag gtcaagatcg gccccagcgg ggccaagatc gccctcgatg cggtgctgta
     3001 ccgacgggcc cggcagcagg ctgcccgagg cggctatgac ctgcttcaca cccacgagga
     3061 ggcgggcttt ttcggggtca atctggcgcg ccgttacggg atgccgcatc tctatgatat
     3121 gcactcatcg ctgccccagc agctgcacaa tttccgcttt tcccgctcgc gccttctggt
     3181 cggggccttc gagcgcctcg aggcgcggac catccaccag gccgcggcgg tgatcaccat
     3241 ctgccccgag ctgcagaact acgtggaaga aaaatttccg ggcaaaacca gcctgctcat
     3301 cgagaacgtg gctgacaatg ctttgctctt cccccccgat gaggccaatg ccgcccggct
     3361 gcggcagcag tacgagttgg gctccagccg catcatcctc tactatggca ccctcgaggc
     3421 ctatcagggc atccccctgc tcattgagag cgccgcgcag ctgttcgctg gcggggggat
     3481 ggacgatgtc cgctttctgc ttgtgggcgg gagcccggag caggtggagg cctgcaaggc
     3541 cctgacgcgg cagcacgggg tggcggagcg cttccgtttc accggctttg tccagcccca
     3601 gctcatcccc gcctttatcg atctggcgga agtgctggtc tcgccgcggc tgagcggcac
     3661 caattctccc ctcaagatct actcctacct gcgatcaggt cgggtgatcg tcgccacgcg
     3721 ccacatcacc cacacccaga tcctcaatga tgaggtcgcc attctggctg atgtcactgc
     3781 cgagggtttt gccgacggga tgcgccgggc gctggtggag cggccagagt cggcggcccg
     3841 gatcgctgcg gcggcggccc tggccgagag ggcgtacagc tatacggagt atctgcgccg
     3901 cgtgcagtgg atcgtggccc atgcggcggg gcagcccaat tgagcggcct cagggagcgg
     3961 gtgcagagcg tacggcgccg gtgctgtaac cggctgcccg ggcagcggac ggggcggggg
     4021 tagacgggta tgtgcggaat ctgcggcatc tacaaccggg agagcggagc agcggtggcc
     4081 ggggccgata taaaggccat gtgcgacgcc atcatccacc gcggccccga tgatgagggg
     4141 cagtacatcc acggccccgt cggcctgggc atgcgccgcc tcgccatcat cgatctcgcc
     4201 accggccgcc agccgatctt caatgaggag ggcgatgccg tcatcgtcct caatggcgag
     4261 atctataacc accttgaact caagcgtgat ctcgaaaagc gcggccaccg tttccgcacc
     4321 cgcagcgaca ccgaggccat cctccacgcc tatgaggagt acggcgagaa ctgtccttcg
     4381 cacctcaacg gcatgttcgc cttcgccatc tgggatgcca ggcgtcagcg cctcttcatc
     4441 gcccgcgacc gcatcggcaa aaagcccctc tactaccatt tcgacggcag gcggctggtc
     4501 ttcgcctccg agatcaaggc gattctgcgt ttgcgggaga tcccgcgcga ggtcgatccc
     4561 cgcgcgctcg acgcttatct gacctatgaa tacatccccg cgcccctcag catcttcaag
     4621 gggatccgca agctgcccca gggccactgg ctcagcctgg accgggaggg gctgcgcatc
     4681 cggcgctact ggcagctgca ctatcgcgcc agcgccgcct ccgaggccga tctggcggcc
     4741 gagtttctcg atctcctgcg cgacgccgtc cgcatccggc tgatgagcga ggtgcccctc
     4801 ggggcttttc tcagcggcgg tctcgattcg agcgcggtgg tggcgatgat gagccagacc
     4861 gccgagaagg gggtgaaaag ctattcgatc ggctttgaca atgccaccta caacgagctg
     4921 ccctgggccc gcatggtggc gcgccacttc ggcaccgagc accacgagga gatcatcacc
     4981 ccggacgccg tggggctgac cgaacgcatt gtccgccagc tcgacgagcc gctcggggat
     5041 ttcagcgtct tcccgacctg ga
//
LOCUS       DAOPWU010000263         5016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16786_length_5016_cov_4.404152, whole genome shotgun sequence.
ACCESSION   DAOPWU010000263 DAOPWU010000000
VERSION     DAOPWU010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16786_length_5016_cov_4.404152"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKM23_11785"
     CDS             complement(<1..265)
                     /locus_tag="PKM23_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNY92194.1"
                     /translation="MPDASSPFITLTFLPAGRRTTVPAGTSILDAAQALGLAITSLCG
                     GAGLCGQCRILLRRGTLPVTHNDLENLTPEELKEGYRLSCSALP"
     gene            complement(376..1224)
                     /locus_tag="PKM23_11790"
     CDS             complement(376..1224)
                     /locus_tag="PKM23_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNY92195.1"
                     /translation="MSASIHIDLHIHSNYSDGLLRPAEIVAYAGKVGLSAIAITDHDD
                     LSAFPEARAAGEKLGLEVIPGIELSAIYAGCETHILGYLYDVTNAPLIAFAEHMRANR
                     IERAREILQRLRHLGMPISFDMVQMRAGQGALGRPHIADIMVEEGYVFSFNEAFQKFL
                     GENKPGYVPKMKLTAEEAIALIHDSGGLAFLAHPGTGTTIEAIEALIEMGLDGLETIH
                     PRHAPEMVDYYRGLARRSGLLETGGSDCHGARRGEMMIGTQSVPREYLEEMRRHLALT
                     NFSESA"
     gene            complement(1255..2259)
                     /gene="tatC"
                     /locus_tag="PKM23_11795"
     CDS             complement(1255..2259)
                     /gene="tatC"
                     /locus_tag="PKM23_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNY92196.1"
                     /translation="MTIDPAQNTYPYEGDSAPKRRRRKAKPAAGESPAQPAAAEAVSA
                     DAGEAVSPDAAPPPADAPSSNSGGTPPPPDDGAGQGPEPAAQDREKRMPFLDHLEELR
                     WRIIWSVLAVIAAAVGCYFFIDEIIAILVHPAPKDIKLIFLSPTEAFMTYIKVAAYAG
                     LVVSLPFVAWQFWRFVMPGLYEKERRAVGPIVVFTVLCFLVGALFAYFLIIPFGLKFL
                     LSYQTDFLVANITIGKYLGFVVTLLLVFGLVFELPVLAYFLSLIGVLTPQFLRSKRRY
                     GILILFIVAAVLTPPDAFSQMMLAIPLLILYEISILVSAAVQRKRKRREAEREREWAG
                     "
     gene            2465..3112
                     /locus_tag="PKM23_11800"
     CDS             2465..3112
                     /locus_tag="PKM23_11800"
                     /inference="COORDINATES: protein motif:HMM:NF019145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92197.1"
                     /translation="MRNLIFVAVPLLLSCAHSIDPASLRLASRQGIEITRSDRLTTPA
                     TVKGALADLGKVKKDKRAETLLLFLKENSDTFKIQAPELELKLLRSDEDELGFTHYRY
                     ARLIREVPVYGDELILHVNNKSQLYMVNGQYHPTQLPESKPALTAEKAGAIALAKGLA
                     HRMESIEQTTLVYYPASEELRLAWHVILRGGLAKWDYFIDAADGRILFDQDRKRS"
     gene            3285..>5016
                     /locus_tag="PKM23_11805"
     CDS             3285..>5016
                     /locus_tag="PKM23_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNY92198.1"
                     /translation="MKWYRYMFMAMLVAFVLPGQAAEQKGKEHQKTRIMNGGPVMARD
                     LRSAGPVWKNPTRLSASKSSYGTLASAVRSEHSLLAAASGRGLKVRELELSPDGTIAF
                     ISGELGRLDQLKSAVIPGVTMQSSVQTTQALRDLQLAQSWLETFAPALKLSQPAAELS
                     LSRYGTDELGKRHLRLQQSWQGLPVWANELYVHVDATDQVYAVNGRYAPTPAGIDPAA
                     ARIDGAAAVEQSRAHLAAEGRLEAIPAALARLTRYSQPVSQKAIWIDRLGTPRLVWQV
                     DINASVRDWYTVMVDAVSGEVLHKYSNTMSEGSVSASGTDLGGASRSFRAYEQSGTYY
                     MLSDLNELSGGVDNLPDNPTGGLWVIDLQNTDATSTSQFYHVTSSSRTSWGDRSAVSA
                     IYNLNKVYDYYKNTHGRRAIDGNASSIITVVNVTEDGRSMDNAYWNGTAIFWGNGADY
                     FTPLAGALDVAAHELTHGVTEYSANLVYQDQPGALNEAMSDFFGCMVDREDWLMGEDI
                     MRSGTGTALRDVSNPHNSQVLSQLPQTMDEYQYTSEDYGGVHTNCGVPLYAMYLFTGS
                     VGKEKAEKIVY"
BASE COUNT         1024 a         1544 c         1616 g          832 t
ORIGIN      
        1 cgggcagggc cgaacaggag aggcggtagc cctccttgag ctcctccggg gtgagatttt
       61 ccagatcgtt gtgggtgacg ggaagcgtcc cgcgccggag cagaatccgg cactggccgc
      121 agaggccggc tccgccgcag agtgaggtga tggcgaggcc gagcgcctgg gcggcgtcaa
      181 ggatggaggt gccggccggc acagtggtcc ggcgtccggc tgggagaaag gtcagggtga
      241 taaaagggga tgaggcgtcg ggcattgggg ctccgcaggt cgaggaagca ctgctacggc
      301 ggcatcaggc cgatgggaag gcgggggctg cggccgcaag gcggtcgagg ctcctgccgc
      361 acccgcccga cccggtcagg ccgattcgga gaaattggtc agggccaggt ggcgccgcat
      421 ctcctcaaga tattcgcggg ggaccgactg ggtgccgatc atcatctcgc cgcggcgggc
      481 gccgtggcag tcggagccgc cggtctcgag caggccgctg cgccgggcga ggccgcggta
      541 ataatcgacc atctccgggg catggcgcgg gtggatggtc tcaaggccgt cgaggcccat
      601 ctcgatgagg gcttcgatgg cctcgatggt ggtcccggtg ccgggatggg cgagaaaggc
      661 gagccccccg ctgtcgtgga tgagggcgat cgcctcctcc gccgtcagct tcatcttggg
      721 cacatagccc ggcttgttct cgccgagaaa cttttgaaag gcctcgttaa agctgaagac
      781 atagccctcc tcgaccatga tatcggcgat atggggccgg ccgagggccc cctggccggc
      841 gcgcatctgc accatgtcga agctgatggg cataccgagg tggcgcaggc gctggaggat
      901 ctcgcgggcg cgctcgatgc ggttggcgcg catgtgttcg gcgaaggcga tgaggggggc
      961 gttggtgaca tcgtaaaggt agccgaggat atgggtctcg cagcctgcgt aaatggcgct
     1021 gagctcgatg ccggggatca cctccagccc cagtttttcg ccggcggcac gggcttcggg
     1081 aaaggccgag aggtcgtcat ggtcagtgat ggcgatggcg gagaggccga ccttgccggc
     1141 ataggcgacg atctccgcgg ggcgcagcag tccgtccgaa tagttggaat gaatatgcag
     1201 atcgatatgg atagaggcgg acaccaggat tcctttccgg ggctgcggac gggatcaacc
     1261 cgcccactcc ctctcccgct cggcctcgcg gcgcttgcgt tttcgttgta cggcagccga
     1321 gaccagaatg ctgatctcgt aaaggatcag cagcggaatg gccagcatca tctggctgaa
     1381 ggcatccggc ggggtcagga ccgccgcgac tatgaagagg ataaggatgc cgtagcgccg
     1441 cttgctgcgc aggaactgcg gcgtcagcac gccgatgagc gagagaaaat aggccagcac
     1501 cggcagctca aaaacgaggc cgaagacgag cagcagggtg acgacaaaac cgagatattt
     1561 gccgatggta atattggcga ccagaaaatc ggtctggtag ctgagcagaa atttcaggcc
     1621 gaaggggatg atgagaaaat aggcgaagag ggccccgacc aggaagcaga ggaccgtgaa
     1681 gaccacgatg ggtccgactg cccggcgctc cttctcatag agtccaggca tgacgaagcg
     1741 ccagaactgc caggcgacaa agggcaggga gaccaccaga ccggcgtagg cggccacctt
     1801 gatataggtc atgaaggcct cagtcggcga gaggaagatc agtttgatgt ccttcggcgc
     1861 cggatggacg aggatagcga tgatctcgtc gatgaaaaaa tagcagccca cggcggcagc
     1921 gatgacggcc aggacgctcc agatgatgcg ccagcgcagc tcctcaaggt gatcgaggaa
     1981 gggcatacgc ttctcgcggt cctgtgcggc cggctctggg ccctggccgg cgccgtcgtc
     2041 cggaggcggc ggcgtaccgc cggagttcga ggacggggca tcggcaggcg gaggggcggc
     2101 gtcaggggag acggcctcgc cagcgtccgc ggagacagcc tccgcagcag ccggctgggc
     2161 gggactttcc ccggcagcgg gtttggcctt gcgacgcctg cgtttggggg cggaatcacc
     2221 ttcgtaggga taggtatttt gggcgggatc gatcgtcaag ggcagctctg cttttgtgat
     2281 tgggccaaga tgcaagatat caattcctga ggaaaatagc aaggcgaatt tggggggcca
     2341 ccggccgcgc gccgaaggat ggggccgctt gccctgaagg ccgggcgcag cagggcgatg
     2401 gaatagccgc cggaaaaaat ctgttgcaga attatacatc atttacatcg ggagaatact
     2461 ctccatgcga aacctcatct ttgtggcggt gccgctatta cttagctgcg cccacagcat
     2521 agatcccgcc agcctccgcc tcgcctcgcg ccagggcatc gaaatcaccc gcagcgaccg
     2581 gctcaccacc cccgctacgg tcaaaggcgc cctcgccgac ctcggcaagg tgaaaaagga
     2641 taaacgggcc gagaccctgc tgctcttcct caaggagaac agcgatactt tcaagatcca
     2701 ggcgcccgag ctggagctga agctcctgcg cagcgatgag gacgagctgg gcttcaccca
     2761 ttaccgctac gcccgcctca tcagggaggt gccggtctac ggtgatgaac tcatcctcca
     2821 tgtcaacaac aaatcccagc tctatatggt caacgggcag taccatccca cccagctccc
     2881 ggaaagcaag ccggccctca ccgccgaaaa ggccggagcc attgccctcg caaaggggct
     2941 cgcccaccgt atggagagca ttgagcaaac cactctggtc tattatccgg ccagtgagga
     3001 gctccgcctc gcctggcacg tcatcctgcg cggcggactg gcgaaatggg attatttcat
     3061 cgacgccgct gacggccgca tcctcttcga tcaggaccgc aaaagaagct gaccctccag
     3121 ccggctggaa aacagcagaa tacgtcatta ccggtcatcc ggtgtggtga tggttcattc
     3181 cacgcaataa ccccgaactt gcattgttaa aggatttaag gaaacacctc cgaccgtatg
     3241 gcgaggcggc agcatttgac agcaataata taaggagaca ggcaatgaaa tggtatcgtt
     3301 acatgtttat ggccatgttg gtcgcctttg ttttacccgg ccaggccgct gaacaaaagg
     3361 ggaaggagca ccagaaaacg cggatcatga acggggggcc ggtcatggcc cgggacctcc
     3421 gcagcgccgg tccggtctgg aagaatccga cccggctctc agcctccaaa agcagctacg
     3481 gcaccctggc cagcgctgtc cgctctgagc actccctgct cgctgctgca agcggccgcg
     3541 gcctcaaggt gcgcgagctg gagctgtccc ctgacggcac gatcgccttt atcagcggcg
     3601 aactcggccg tctggaccag ctcaaatcgg ccgtcattcc cggcgtgacc atgcagtcat
     3661 cggtgcagac gacccaggcc ttgcgcgacc ttcagctggc ccagagctgg ctcgaaacct
     3721 tcgcgccggc cctgaagctc agccagcccg ccgctgaact ctcgctcagc cgctacggaa
     3781 ccgatgagct cggcaagcgc cacctccgct tgcagcagtc ctggcagggt ctgccggtgt
     3841 gggccaatga actctacgtc catgtggatg ccaccgacca ggtctacgcc gtcaacggcc
     3901 gctacgcccc gaccccggcc ggcatcgatc cggcggcagc ccgaatcgac ggcgccgctg
     3961 cggtcgaaca gagccgcgcc cacctcgcgg cggaaggccg gctcgaggcg atccccgccg
     4021 ccctcgccag gctgacgcgc tacagccagc cggtcagcca aaaggcgatc tggatcgacc
     4081 ggctgggcac cccccgcctg gtctggcagg tcgacatcaa cgccagcgtc cgcgactggt
     4141 atacggtcat ggtggacgcc gtcagcggtg aggtgctgca caaatacagc aacaccatga
     4201 gcgaaggctc cgtcagcgcc tccggcaccg atctcggcgg tgccagccgc tccttccgcg
     4261 cctatgaaca gagcggcacc tattatatgc tcagcgacct caacgagctc tccggcggtg
     4321 tggacaacct cccggacaat cccaccgggg ggctctgggt catcgacctc caaaacaccg
     4381 acgcgacctc aacctcccag ttctatcacg tcacctcctc ctcgcgcacg agctggggcg
     4441 accgcagcgc ggtctcggcg atctataatc tcaacaaggt ctacgactat tacaagaaca
     4501 cccatggccg ccgggccatc gacggcaacg cttccagcat catcaccgtg gtcaacgtca
     4561 cagaggacgg caggtcgatg gacaatgcct actggaacgg tacggctatc ttttggggca
     4621 acggcgccga ttatttcacc cccctcgccg gggccctgga cgtggccgcg cacgagctga
     4681 cccacggcgt caccgaatac tcggccaacc tggtttatca ggatcagccc ggcgccctca
     4741 acgaggcgat gtccgatttc ttcggctgca tggtcgaccg tgaggactgg ctgatgggcg
     4801 aggatatcat gcggtcgggg acgggcacag ctctgcgcga cgtctccaat ccccataact
     4861 cccaggtgct cagccagctg ccgcagacca tggatgagta tcaatatacc agcgaagatt
     4921 acggcggcgt gcataccaac tgcggcgtcc ccctttacgc catgtatctg tttaccggca
     4981 gcgtcggcaa ggaaaaggcg gaaaagatcg tttacc
//
LOCUS       DAOPWU010000264         4980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16957_length_4980_cov_5.473096, whole genome shotgun sequence.
ACCESSION   DAOPWU010000264 DAOPWU010000000
VERSION     DAOPWU010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16957_length_4980_cov_5.473096"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3190)
                     /locus_tag="PKM23_11810"
     CDS             complement(<1..3190)
                     /locus_tag="PKM23_11810"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNY92199.1"
                     /translation="MKNNRVVRGIGLSALIVMLIMSMPLQAQPLRMSASAAVTQAPFG
                     PPPFSNDWQRVSIKIDTSQFDVDEEVLDAILANVQTFRICTEMSDYRDTGAIDSLRIG
                     SRFAASYNEGTEGWSAAGDGTLSWSSAGGVGGGFLQISDWGTGDYHWAVAPPSWAGDW
                     SALKGSYITFYYKTDYPDYGAVIEIGSGENTQLVLSADKMTVPLGTSTIARVALPAAA
                     ISDVVITLTSSEPSCLQVPKAVTISRGTSETEFSVAMGTSAATGCSAVISASAEGYQT
                     SRLTMNVGKSADAGKYATLTGRVTDAITKAGIPGAVVWIAGRSAETDSAGGYTIEDIP
                     TSVIAANFSAEPLSGKAPLLVQFNDLSSVGSYALGVTAAGYMGTESILAFAEGEDKYI
                     EISLTPVIKPGEMRLILNWGGQPLDLDLHLHTPVIDGRSHEIYWDNKGHLTSAPYVYL
                     DVDHQEGYGPETITIGELQPGTYRCYVENYSTIPAISSARAQVQIYTSDGLLQTIAIP
                     TSGTGLFWYVCDIDGATGAVTVHNQIQAGTPATTLAAANAKIPAGHTIIGSGGILSWS
                     WDFDNDLFIDSYTQNPQYTFNKPGLYTVTLRVSDGSREYVVRKENYITVLPPVITDVS
                     WRKQGAPSGEDLYAVWAVDTLQAWAGGSGGTLLYTANGGATWTTRNVAAQFTLRDLFF
                     LNHKSGWAVGNDSKQNAVILKSEDGGLSWTRVLSSNSARLYSLHMINGTTGWISGHEG
                     KIETTGDGGATWAQQFTGLAAGLQAIYFLNAEKGVAAGENGVILRTGDGGANWQLMTS
                     GVSARINDIWFADENLGWAVCEDGKVLTSNNGGATWSAKQVSTLALRAIHFNSGYHGY
                     IAGDGGRIFKTYDGGDSWKEDNSTTAQTLNDLFIAHPACAWAVGAGGTILKLQRGSLY
                     TGAVTEMSAAATGTNSIRLSWRNPDDDFAGTVIVRSTTGYPASATDGVVIYNGTSSLV
                     TDTPLAANTTYYYSAFAYNSAGSFSPPSSSARASARTEEGFNLFGYSVTIGSIDAGAF
                     PTVKSFVSVVDSVTLEPIAELTADNFGVREDGT"
     gene            complement(3288..4112)
                     /locus_tag="PKM23_11815"
     CDS             complement(3288..4112)
                     /locus_tag="PKM23_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HNY92200.1"
                     /translation="MFAAKRVIPLLAVGLLLALAGACEKPLSMAPDYSGVSEATATFA
                     LSKATLLETGISRLVISVTGEGMKAVTVDTAITAEGQFRTDLKISPGKNRLFRVSAYQ
                     DTLLVLAGRDSLNLKEGQKVTLHMKLDFQVPALTLTPIDTTLARNSTFTVYVQAHHVD
                     SLCTIGTKLLFDPARLQVVELGREDDFLKKNGGAVTQLQFSRDNTAGEVKLLLGVFPA
                     GKSVSGEGKIARIVFQAVGSSTAEIDLSLNGVDLGLYDQHAVLMPAVALGSRITIQ"
     gene            complement(4173..>4980)
                     /locus_tag="PKM23_11820"
     CDS             complement(4173..>4980)
                     /locus_tag="PKM23_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNY92201.1"
                     /translation="TRRLLFALLIMPLLAAAQVFDTTPPRIAHQPVRLGRVGKVLPII
                     ANVSDNGGVKSVRITIQHDGQEIQHQMNQVKSESAIPVVVQTAAEGVVVNSLPGGSGK
                     SLARLGAGEQLEVTLVRAPYYRIRTAAGVVGYVHGDEVEAVESGASYRVILPAQLTAG
                     GRLAYKIAAVDDFGNESQTDLIPVRLITDEELARLQSQKPGQTPSRPVESAESAARPG
                     EPNKSIKSIFSRPVFWIATAAAGGGLYYFLSSGDEESSDKKASVGLVVGW"
BASE COUNT          912 a         1474 c         1617 g          977 t
ORIGIN      
        1 tggtgccatc ctcgcgcacc ccgaaattat cggcggtgag ttcggcgatc ggctccaggg
       61 tgaccgaatc gacgacgctg acgaaggatt tgaccgtcgg gaaggcgccg gcgtcgatgc
      121 tgccgatggt gaccgaatag ccgaagaggt taaagccctc ttcggtcctg gctgaggccc
      181 gggcggaaga ggagggcggg gagaagcttc cggcgctatt gtaggcgaag gcgctgtaat
      241 aataggtggt gttggcagcc agcggggtat cggtgacgag gctgctggtg ccgttgtaga
      301 tgacgacgcc gtcggtggcg ctggcgggat agccggttgt actgcgaacg atgacggtgc
      361 cggcgaaatc atcgtcggga ttccgccagg agaggcggat ggagttggtg ccggtcgccg
      421 cggcgctcat ctccgtgacc gcgccggtgt agagcgagcc gcgctggagt ttgaggatgg
      481 tgccgccggc gccgaccgcc caggcgcagg ccggatgggc tataaagagg tcattgaggg
      541 tctgggcggt ggtgctgttg tcctctttcc aggagtcgcc cccgtcatag gtcttgaaga
      601 tgcggccgcc gtcgccggcg atatagccgt ggtagccgga gttgaaatgg atggcgcgca
      661 gggccagggt gctcacctgt tttgcgctcc aggtggcgcc gccgttgttg ctggtcagca
      721 ccttgccgtc ctcgcagacg gcccagccga ggttttcgtc ggcaaaccag atgtcattga
      781 tgcgggcgct caccccgctg gtcatcagct gccagttggc gccgccatcg ccggtgcgca
      841 gaatgacgcc attctcgcct gccgcaacac ctttttcagc attgagaaaa tagatggcct
      901 gcagtccggc ggcgaggccg gtgaactgct gcgcccaggt ggcgccgccg tcgccggtgg
      961 tttcgatctt gccttcatgg ccgctgatcc agccggttgt gccgttgatc atgtgcaagc
     1021 tgtaaagccg ggccgaattg ctgctcagga cgcgggtcca ggagaggccg ccgtcttcgc
     1081 tcttcaggat gacggcgttc tgtttgctat cgttgccgac cgcccacccg ctcttgtggt
     1141 tgagaaaaaa gaggtcgcgc agggtgaact gtgcggccac attgcgggtt gtccaggtgg
     1201 cccccccgtt ggcggtatac agcagcgtgc cgccgcttcc gcccgcccag gcctgcaggg
     1261 tgtccaccgc ccagacggca tagagatcct cgccggaggg tgcgccctgc ttgcgccagg
     1321 agacatcggt gatcacaggg ggcaggacgg tgatatagtt ctctttccgg accacatatt
     1381 cccggctgcc gtccgagacc cgcagggtga cggtgtagag ccccggtttg ttgaaggtat
     1441 actgcgggtt ctgtgtatag gaatcgatga agagatcgtt atcaaagtcc cagctccagg
     1501 agagaatacc tccgctgccg atgatcgtat ggccggccgg gatcttggcg ttggcggcgg
     1561 ccagtgtggt cgcgggcgtg cccgcctgga tttgattgtg caccgtcacc gccccggtgg
     1621 cgccatcgat gtcgcagaca taccagaaga gaccggtgcc gctggtgggg atggcgatgg
     1681 tttgcagcag tccgtccgag gtgtagatct gcacctgggc cctggcgctg ctgatggcgg
     1741 gtatggtcga ataattctcg acatagcagc gataggtgcc cggctgcagt tccccgatgg
     1801 tgatggtctc cggaccatag ccctcctgat gatcgacatc gagatagaca tagggcgctg
     1861 aggtgagatg ccccttgttg tcccaataga tctcatggct gcggccgtcg atgaccggtg
     1921 tgtgcagatg gaggtccaga tccaggggct gtccgcccca gttgagaatc agacgcattt
     1981 cgccgggctt gatgaccggg gtcagggaga tttcgatata cttgtcctcg ccctcggcga
     2041 aggcgagaat tgactccgtg cccatatagc ccgcagctgt caccccaagg gcatagctgc
     2101 cgacgctgga gaggtcgttg aactgcacaa ggaggggagc cttgccggag aggggctcgg
     2161 ccgaaaagtt ggcggcaatg acgctggtcg ggatatcctc gatggtgtag ccgccggcgg
     2221 aatcggtctc cgcgctccgg cccgcgatcc agaccaccgc gccgggaatc cccgccttgg
     2281 tgatcgcatc agtgacgcgc ccggtcaggg tggcgtattt gccggcatcg gccgatttgc
     2341 ccacattcat ggtcaggcgg ctggtctgat aaccctccgc actggccgag atcaccgccg
     2401 agcagccggt ggcagcggag gtgcccattg cgaccgaaaa ttcggtctcg ctcgtgcccc
     2461 tgctgatggt caccgccttg ggcacctgca ggcaggaggg ttcggaagaa gtcagggtga
     2521 tgaccacatc gctgatggcg gcggccggga gggcgaccct ggcgatggtg ctcgtgccca
     2581 ggggcaccgt catcttgtcc gcgctcagga cgagttgggt gttctccccc gagccgattt
     2641 ctatgaccgc gccatagtcg ggataatcgg tcttgtaata aaaagtgatg taggagccct
     2701 tgagggcgga ccaatcgccc gcccaggagg ggggcgccac cgcccaatgg taatcgccgg
     2761 tgccccagtc gctgatctgg aggaagccgc cgccgacccc gccggccgaa ctccagctca
     2821 gggtcccgtc acccgcagcg ctccagccct cggtcccctc gttgtaggag gctgcaaaac
     2881 gagaaccgat gcggagactg tcgatggcgc cggtgtcgcg gtaatcgctc atctcggtac
     2941 agatgcggaa ggtctgcaca ttggccagaa tggcgtcaag cacctcctcg tcaacatcga
     3001 actggctggt gtcgatcttg atggagacgc gctgccagtc gttggaaaac ggcggcggac
     3061 cgaagggggc ctgagtcaca gccgctgaag cgctcattct gaggggctgc gcctgcagcg
     3121 gcatgctcat gatgagcatt acgatcagag ctgaaagccc gatgccccga accacacgat
     3181 tatttttcat attcaactct tcagttaatg cgacgattca tatcggtctc cccggcgagg
     3241 gcccgtccat ccgggccgca cgggagggat tagcggtcta ttctccgtca ctgaatggtg
     3301 atacggctgc cgagcgccac ggccggcatc agcacggcat gctggtcgta gaggccgaga
     3361 tcgaccccgt tgaggctgag atcgatctcg gcggtcgaac tgcccaccgc ctgaaagaca
     3421 atgcgggcga tcttgccctc tccactcacg gatttgcctg ccggaaagac gcccaacagc
     3481 agcttgacct ctccagctgt attatcccgg ctgaactgca gctgggtgac cgctcccccg
     3541 tttttcttga gaaaatcatc ctcacggccg agttcgacca cttgcagcct ggcaggatca
     3601 aaaagcaact tggtgccgat ggtgcaaagg ctgtcgacat ggtgggcctg gacgtaaacg
     3661 gtgaaggtgc tgttcctggc cagggtggta tcgatcgggg tgagggtgag ggcgggaacc
     3721 tgaaaatcga gcttcatatg aagggtcacc ttctgcccct ctttcagatt gaggctgtcc
     3781 ctgccggcga gaacaagcag ggtatcctga taggcggaga cccggaagag gcgattcttg
     3841 ccgggggaaa ttttgagatc ggtcctgaac tgaccctcag cggtgatcgc agtatcgacc
     3901 gtgaccgcct tcatgccctc tccggtgacg gagatgacca gccggctgat gccggtttca
     3961 agcagggtcg ccttgctgag ggcaaaggta gcggtggctt cgctcacccc ggaatagtcc
     4021 ggcgccatcg acaggggttt ttcacacgcg ccggccaggg ccagcagcag gccgacggct
     4081 aggagaggaa tcactctttt ggctgcaaac atgctctgac tcctatggat aaaatagatc
     4141 atattcttcg ctgcctgtta tgcacggtca gttcaccagc caacgaccag cccgaccgag
     4201 gccttcttgt ccgaactctc ttcatctccg ctgctgagaa aataatagag gcctccgccg
     4261 gccgccgcag tggcgatcca gaaaaccggt cgtgaaaaaa tcgatttgat cgacttgttc
     4321 ggctccccgg gccgggctgc agactctgca ctttcgacag gcctggaggg cgtctggccc
     4381 ggtttctgac tctgcaaacg ggccagttcc tcatcggtga tcaggcggac cgggatcaga
     4441 tcggtctggc tctcattgcc aaaatcatcc accgcggcaa ttttataggc cagccgcccc
     4501 cccgccgtca gctgcgccgg caggatcacc cgataggagg cgcctgattc aacagcctcg
     4561 acctcatcgc catggacgta gccgaccacc cccgcagcgg tgcggatgcg gtagtagggc
     4621 gcccgcacca gggtcacctc cagctgctcg ccagcaccga gccgggcgag actcttgccc
     4681 gaacctccgg gcaatgagtt gaccacaacc ccctcagcag ccgtctggac cacaacaggg
     4741 attgcactct cgctcttgac ctggttcatc tgatgctgaa tctcctgccc gtcatgctgg
     4801 atggtgatac gcacgctctt gacccccccg ttgtcgctga cattggcgat gatcggcagc
     4861 accttgccca cccggcccag acggaccggt tgatgggcga tccggggcgg ggtggtgtcg
     4921 aatacctggg cggcggcgag caggggcata attaaaagcg cgaaaagcag gcgccgcgtt
//
LOCUS       DAOPWU010000265         4975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_16974_length_4975_cov_4.926829, whole genome shotgun sequence.
ACCESSION   DAOPWU010000265 DAOPWU010000000
VERSION     DAOPWU010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16974_length_4975_cov_4.926829"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..831)
                     /locus_tag="PKM23_11825"
     CDS             complement(25..831)
                     /locus_tag="PKM23_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY92202.1"
                     /translation="MTGLAVQEGQAGEISNADGAHPRVSVVMPCLNEEEGIAASIRAV
                     KEVFQQERIDGEIVVSDNGSTDDSRRIALSEGCRVVDQPERGYGAAYMKGLSEARGEF
                     IIMADADHTYDFNAIPDFLRLLQEGNDFVLGSRFKGKIHPGAMPWARRYIGNPVLSTM
                     TRLLFRTRLSDIHCGMRGLRRSALADLGLKMPGMEFATEMVVSVLRNGLRVAETPVDY
                     FPRKGASKLNPLHDAWRHVIFMIRNGPSWLWCVPAFAGLAAVVILILVWI"
     gene            complement(828..1814)
                     /locus_tag="PKM23_11830"
     CDS             complement(828..1814)
                     /locus_tag="PKM23_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNY92203.1"
                     /translation="MKAVEFWRFVNRPDRPVILLIVTSTSGATPGKTGFRMAADAEGG
                     LVGSIGGGKLEYDLVEEARALLRMTSPAPLLRHLHLVEEGTPEANGMLCGGEQTVLLY
                     PVRDEDRPVIDEIISALSSGASGLWSVTGTGMHWQEEGPASARAEQEGPAGLFYQERI
                     FPRETLFIIGGGHVSLALSRAMSLLDWRLVLLDDRPEVDTMKNNHWADEKIIAPFAEV
                     ARHVPPGGRSWVVIMTPSHRADEEVLRRLVAHPFRYLGMMASPAKAAEILSRLRAEGV
                     EEALLRRVFTPVGLPISSHTPAEIAVSIAAQLIQIRNQTPTHEQETTGSKGL"
     gene            complement(1860..4133)
                     /locus_tag="PKM23_11835"
     CDS             complement(1860..4133)
                     /locus_tag="PKM23_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNY92204.1"
                     /translation="MSSSPFDAHLHVRGESQFIDDRLVPAGTLHAAVLTSPVARGRLL
                     ALDGTAALAAPGVRSLLTARDIPGENQIGGILPDEVLLAGAELDFIGQPVALIAADSA
                     AEARSAVSLIRLEYEEEPPLLDAREAARRGEFIQPPRVFTLGDVESAWSRCDVILEGR
                     ADSGGQEHLYLETQGALAWPEEQGGIKLISATQSPTTVQRTVARVLGLPMHKIEVDVL
                     RLGGAFGGKEDQATAWAAMAALAAFHLHRPIKLVLRRHEDMRWTGKRHPYSSDYKIGL
                     TRAGKILAWEVTYYQNAGAAADLSPAILDRTLFHATNCYVIPNLRATGLCCRTNLPPN
                     TAFRGFGGPQAMFVLEAAIDQAAQRLGLPAAQIQAANLLDEGYEFPCGQRAHNAQARR
                     CWQELEQRCRPADRRQAIDRFNAGHEELKKGMAIMPICFGISFTNTMLNQANALVHIY
                     NDGSVGVSTGAVEMGQGVHEKLRAIAARCLGIRIERIRIESTNTTRNANTSATAASTG
                     ADMNGHAVRIACEVLLARLRPFIARLAGLDQEGLVTIRREQIQAGERLLPYTWEKLIA
                     AAWAARISLSAHGHYATPGLHFDRVINKGEAFAYHVYGAALVEATIDTLRGTCRFDAV
                     RIVHDAGCSLNPGIDRGQVEGALLQGLGWMTLEEVLHDDKGRLLTDSMTTYKVPDLFF
                     APGEVELHWLERSEGGNGPFRSKAIGEPPLMYGIGGWFALLDALRAARPGLELPYISP
                     MTHERLLMALHTGETAG"
     gene            complement(4130..>4975)
                     /locus_tag="PKM23_11840"
     CDS             complement(4130..>4975)
                     /locus_tag="PKM23_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNY92205.1"
                     /translation="QLAALSQSRHTAITGRPVVVAGGTDLYVQKPARLESAPVELLSE
                     RPDLRGIEIQGSWCRVGGGVTVEELRLASELRAFLPDLPDFLRLVSSTQIRNRATVAG
                     NLVNASPIGDLTILLLALEAEVDLHDGAALRTLPLRRFYLGYKKLARREEELVTAVRF
                     PLLQPGDHFNFEKVSRREHLDIASVNSACLLRMVGGVVEEIHLSAGGVAPIPLCLEKC
                     SSYLRGRELEEATLAEALRVAGDEIAPISDVRGSAEYKRLLLRRLLRAHLTPFLPERR
                     QGRRP"
BASE COUNT          863 a         1532 c         1766 g          814 t
ORIGIN      
        1 cacaggctgg agaaaaaata caccctaaat ccagacaaga atcagaatca cgacggcggc
       61 caagccggcg aacgcgggaa cgcaccagag ccaggacggg ccattgcgaa tcatgaaaat
      121 gacatggcgc caggcatcgt gaagaggatt gagcttggat gcgcccttgc gcgggaaata
      181 atcgaccgga gtctccgcaa cccgcagccc gtttctcagg acagagacca ccatctcggt
      241 ggcgaattcc atacccggca tcttcagccc gagatccgcc agggcgctgc ggcgcaggcc
      301 gcgcatgcca cagtggatgt cggagaggcg ggtgcggaag aggaggcggg tcatcgtcga
      361 aagcaccggg ttgccgatgt agcggcgggc ccagggcatt gctcccggat ggattttccc
      421 cttgaagcgg gaacccagga caaaatcatt accctcctgc agcagcctca aaaagtcagg
      481 gatggcgtta aagtcatagg tatggtcggc gtccgccatg atgatgaact cgccgcgcgc
      541 ctcgctgaga cctttcatgt aggcggcgcc atagcctctc tccggttgat ccaccacccg
      601 gcagccctct gaaagcgcga tccggcgcga gtcgtccgtg gagccattat cgctgaccac
      661 gatttcgccg tcgatccttt cctgctggaa aacctctttg acggcgcgga tgctggcggc
      721 gataccctcc tcctcattga gacagggcat gacgaccgac acccggggat gtgcgccgtc
      781 cgcgttactg atctcccccg cctgaccttc ctgtactgcc aatcctgtca taagcccttg
      841 cttcctgtcg tttcctgttc gtgtgttggc gtctgattgc gaatctggat gagctgggcg
      901 gcgatgctga ctgcgatctc ggccggtgta tggctggaga tcggcagccc caccggggtg
      961 aatacccgcc gcagcagggc ctcctccacc ccctccgcgc gcaggcggga gaggatctcg
     1021 gccgccttgg ccgggctggc catcatgccg agatagcgga aaggatgggc gaccagcctg
     1081 cgcagcacct cctcgtcggc ccggtgcgag ggggtcatga tcaccaccca ggagcggccg
     1141 ccgggcggga catgacgcgc cacctcagca aagggggcga tgatcttttc gtccgcccag
     1201 tgattattct tcatggtgtc gacctcagga cggtcgtcga ggaggacaag gcgccagtcg
     1261 agcaggctca tcgcccggga gagggcgagg ctgacgtggc cgccgccgat gatgaagagg
     1321 gtctcccggg ggaagatgcg ctcctgatag aagagcccgg ccggcccctc ctgctctgcg
     1381 cgggcggaag cggggccctc ctcctgccag tgcatcccgg tgccggtgac cgaccaaagg
     1441 ccggaggcgc ccgaagagag ggcggagatg atttcgtcga tgaccgggcg gtcctcatcc
     1501 cgcaccggat agagcagaac agtctgttcg ccgccgcaga gcatgccgtt ggcctcggga
     1561 gtgccctctt ccaccaggtg cagatgcctc aagagcgggg ccgggctcgt cattctgagc
     1621 agggctcgcg cctcctcgac caggtcatac tccagcttgc cgccgccgat ggagccgacg
     1681 agccccccct ccgcatccgc cgccatcctg aagccggtct tgccaggggt ggcgccgctg
     1741 gtactggtga cgatgagcag gatgacgggt cgatcaggtc tattcacaaa acgccagaat
     1801 tcaacggcct tcatgcgatt cttccttcac aggggtgcag catgatctgc aagcgggatt
     1861 cagccggctg tctcgccggt atgcagggcc atgagcagac gctcatgggt catgggagag
     1921 atatagggca gctcgaggcc ggggcgggcg gcgcgcaggg catcgagcag ggcgaaccag
     1981 ccgccgatgc cgtacatgag ggggggttcg ccgatggcct tgctgcggaa gggcccgttt
     2041 cccccttcag agcgttccag ccagtgcagc tcaacctcgc cgggggcgaa aaagaggtcc
     2101 gggaccttgt aggtggtcat cgagtcggtg agtagccgcc ccttgtcgtc atggagcacc
     2161 tcctcgaggg tcatccagcc cagcccctgc aggagagcgc cctccacctg gccgcggtcg
     2221 atccctggat tgagggagca gccggcatcg tggacgatgc ggacagcgtc gaagcggcag
     2281 gtgccgcgca gggtatcgat ggtcgcttcg acgagagcgg cgccataaac atgataggcg
     2341 aaggcctctc ccttgttgat gacgcggtcg aagtgcaggc ccggcgttgc gtaatggcca
     2401 tgagcgctca ggctgatgcg cgccgcccag gcggcggcga tgagcttttc ccaggtgtaa
     2461 gggagcagcc gctccccggc ctggatctgt tcgcggcgga tggtcaccag cccctcctga
     2521 tccagcccgg cgaggcgggc gataaagggg cgcaggcgcg ccagcagaac ctcgcaggcg
     2581 atccgcaccg catgaccgtt catgtcggcg ccggtgctcg cggctgtggc cgaggtgttg
     2641 gcgttgcggg tggtattggt gctctcgatg cggatgcgtt cgatccggat gcccaggcag
     2701 cgggcggcga tagcgcgcag tttttcgtgc accccctgcc ccatctcaac cgcgccggtg
     2761 ctgacgccga ccgagccgtc gttgtagata tggaccaggg cgttggcctg gttgagcatg
     2821 gtgttggtga aggaaatgcc gaagcagatc ggcatgatcg ccattccttt tttgagctcc
     2881 tcgtggccgg cgttgaagcg gtcgatggcc tggcgcctat cagcgggacg gcagcgctgc
     2941 tccagttcct gccagcagcg ccgggcctgg gcgttgtgag cacgctggcc acaggggaat
     3001 tcatagccct catcgagcag gttggccgcc tggatctgcg cggcggggag gccgagccgc
     3061 tgcgccgcct gatcgatggc ggcctcaagg acgaacatcg cctggggccc gccgaagccg
     3121 cggaaggcgg tgttgggcgg gaggttagtg cggcagcaga ggcccgtggc gcgcagattg
     3181 ggaatgacgt aacagttggt ggcgtgaaag agggtgcggt cgaggatggc cggggagagg
     3241 tcggcggccg cgccggcgtt ctggtaatag gtaacctccc aggcgaggat cttgcccgcg
     3301 cgggtgaggc cgatcttgta atccgaggag tagggatggc gcttgccggt ccagcgcata
     3361 tcctcgtggc ggcgcagcac cagcttgatc gggcggtgga ggtggaaggc ggcgagggcg
     3421 gccattgcgg cccaggcggt ggcctgatcc tccttgccgc cgaaagcccc gccgaggcga
     3481 agcacatcga cctcgatttt gtgcatgggc aggccgagca cgcgcgccac ggtgcgctgc
     3541 accgtggttg gcgactgggt ggccgagatc agcttgatgc ccccctgctc ctccggccag
     3601 gccagggctc cctgggtctc gagataaagg tgttcctgtc cgccgctgtc ggcgcggccc
     3661 tcgaggatga catcgcaccg gctccaggcg ctctcaacgt cgccgagggt gaaaacgcgg
     3721 gggggctgga tgaactcgcc gcgccgggcc gcctcgcgcg catcgagcag ggggggctcc
     3781 tcctcgtatt caaggcggat gagagacacg gcgctgcgcg cctcggcggc ggagtcggcc
     3841 gcaatcagcg cgacaggctg gccgatgaaa tcgagttcag cgccagccag cagcacctca
     3901 tcggggagga tgccgccgat ctggttctcg ccggggatat cccgggcggt gaggaggctg
     3961 cgcacgcccg gggcggccag ggcggccgtg ccgtcgaggg cgagcaggcg gccgcgggcg
     4021 accggtgagg tcaggaccgc ggcgtgcagg gtgccggcgg gcacgaggcg gtcgtcgatg
     4081 aactgggact cgccgcggac gtggagatgg gcgtcaaagg gagaggaact catggcctcc
     4141 tcccctgccg gcgctccggc aggaatgggg tgagatgggc ccgcagcagc cggcgcagca
     4201 gcagccgctt gtactccgcc gaaccgcgca cgtcgctgat cggagcgatc tcgtccccgg
     4261 ccacgcgcaa ggcttccgcg agggtcgcct cctccagctc gcgcccgcgc aggtaggagc
     4321 tgcacttttc caggcagagg gggatggggg ctacgccccc ggcggagaga tggatctcct
     4381 caaccacacc tccaaccatg cgcagaaggc aggccgagtt gacgctggcg atgtcgaggt
     4441 gctcgcggcg gctcaccttt tcgaaattga aatgatcccc gggctggagc aggggaaagc
     4501 gcaccgccgt gaccagctcc tcctcgcggc gcgccagctt tttatagccg agatagaagc
     4561 gacgcaaggg gagggtgcgg agggcggcgc cgtcgtgaag atcgacctcg gcctcgagag
     4621 caaggaggag aatggtgaga tccccgatgg gtgaggcgtt gacgagattg ccggcgacgg
     4681 tggctcgatt gcggatctgg gtcgatgaga ccagccggag aaaatcgggc agatcgggaa
     4741 ggaaagcgcg gagttcggac gcaaggcgca gttcctcgac ggtgacgccg ccgccgaccc
     4801 ggcaccagct gccctgaatt tcgatcccgc gcagatcggg ccgctcgctg agcagctcca
     4861 ccggggccga ttcgagccgc gcgggctttt gcacgtagag gtcggtgccg cctgcgacca
     4921 cgacgggccg gccggtgatg gcggtgtgac gggattggct gagggctgcc agctg
//
LOCUS       DAOPWU010000266         4961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_17032_length_4961_cov_3.581329, whole genome shotgun sequence.
ACCESSION   DAOPWU010000266 DAOPWU010000000
VERSION     DAOPWU010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17032_length_4961_cov_3.581329"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="PKM23_11845"
     CDS             <1..1499
                     /locus_tag="PKM23_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNY92206.1"
                     /translation="FRMDFSVPPSTQDEKVDERGNKIVRAIWNKPQSLVTSTLRFTAG
                     NSTELKPLRTSAPFPLVQIGRVEEAYLKSTGQAPSDNEEIARLSAQLTAGSKTEFDAV
                     KRILTYVVDHLHYVLAPPSYDALYSLRTGKGNCQNYSHLSAALMRAVGIPCRIVNGVT
                     LKEPYDVELTNGTLTLRMAQGRHSWIEVWFPDLGWVPFDPQQTALYVSNRFIRIEFGL
                     DNEETCNDGLIRWSQVQGAEGNPQFEETINYVLASDRVNLRAEKQNYGPQKLLFFPAV
                     EAAFIPVAARSSLPPAPKPRLTEQKIMRRIAYSQPFSQGNTEFPQNTDFLSARGPARL
                     AESGEMEMRKNFMVETAEYVTTQGNQYAQTFILEKPLKLTRIGLALHKFGGSGQLWLE
                     ILRDDGEGKPGEYIATSRYLALEEIKYAPGYDWVDFDFGADELLLPPGRYWMALGFTG
                     SPIINWFFSYGKPVGPEDGTRYKTLFDETWSRSLAYEFNYRIIGLTGE"
     gene            1551..3161
                     /locus_tag="PKM23_11850"
     CDS             1551..3161
                     /locus_tag="PKM23_11850"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNY92207.1"
                     /translation="MTEKYILTHDLGTSSNKAVLFTLYGRLVAEARHDYSVHYPQPSY
                     AEQDPFDWLHAVYDSTHKAMEIAGISPDQIAGITFSCQMQTLVAVDAQGMPVMPAISW
                     LDTRGREILYDRLWTRPRIIGYQPLRLLRFLRVTGGAPGHTGKDPIAKIIWLKEKRPD
                     LYAKTAKFLDVKDFVIYHLTGTWCKSVDLAVVWWLLDTRKNRNQWDEKLCRLAGIDPE
                     LLPPALPSAAIVGTLQEEAARLMGLKPGIPIINGSGDIPAAAVGSGALHEGELSIRLG
                     TSGGIAGHFSRRKIDLAHYAGCIGSTYPEKYYLALAHQETIGICLEWLANRILYHKQR
                     LSEETLFEDVYQLLDHLAAQAPPGAAGLIFTPWMFGERCPIDDHNVRAGLFNLSLQHG
                     REHLIRAVLEGIAFNLRWGLEVLEKLYQPVDRLHIIGGGAKSDIWCQIIADITNRTIC
                     QADNPQQANARGAAMLASLSLGHIKSFEAISEHIVIRQRYTPDPKNRQLYDHLFREFK
                     KLYRQNKSWYARMNGETGWALTNRGVQP"
     gene            3207..4244
                     /locus_tag="PKM23_11855"
     CDS             3207..4244
                     /locus_tag="PKM23_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY92208.1"
                     /translation="MSQRATGSILIVDDEAIIRDSLQQWLEIEGFRVFSAEDGERALQ
                     LCRFERLDVGVFDIRMPGMDGITLLNRTRSCRPEMDVIMMTAFASIEDAVRCISEGAH
                     DYIIKPFPPEKLSKSIRHLLESRKLHASQQDLISQQGELRRFIAACTPYLALGAATAV
                     LSGAPWAEQTGGGEAALEPSLLKELIDRALEISRPAEQTPSSNLRSVVETALLLAETP
                     PEGRARLSPAPPATEMSAGIPFVPAILALQLLFEHLLRHGHPDPIINLEAEKSAPLRP
                     VLRIGLAAPMSVKDRAQLLEQQEGGASDSSRLRLAATLLRILGVAVTVTPRREEGETI
                     LLIFPSAEVNR"
     gene            4251..>4961
                     /locus_tag="PKM23_11860"
     CDS             4251..>4961
                     /locus_tag="PKM23_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNY92209.1"
                     /translation="MTNEVKILIVDDEELLREGCRRLLEMSGYTVRTAGEADSGLRLA
                     AADAFEIAFVDFRMPGMSGIDFIRRMQEISPATDVVMMTGYASIEMAVEAMKNGARDY
                     VAKPFEAEQILEIVRKLLQNRQTPTAPAGAGFSFDFNGRPLQIIGGGGRMQELFALIR
                     KVAPTESTVLVQGESGTGKELVAKAIHALSPRKDKAFFAMDCGSLVESLFESELFGHV
                     KGSFTGATATKHGAFELAH"
BASE COUNT         1017 a         1695 c         1316 g          933 t
ORIGIN      
        1 ccttccgcat ggatttctcg gtaccgccct ccacccagga cgaaaaggtg gacgaacgcg
       61 gcaacaagat tgtccgcgcc atttggaaca aaccccagtc cctggtgaca tctaccctgc
      121 gcttcactgc aggcaacagc accgagctca agcccctgcg caccagcgcc ccttttcccc
      181 tcgtccagat tggcagggtg gaggaggctt atctcaaatc caccggacag gcgccctccg
      241 acaatgagga gatcgcccgc ctgtccgccc aactcaccgc cggcagcaag accgaatttg
      301 atgccgtcaa gcgcatcctc acctacgtcg tcgatcatct ccactacgtc ctggccccgc
      361 ccagttatga cgccctctat tccctgcgca caggcaaggg caactgccag aactattccc
      421 atctcagcgc cgccttgatg cgcgccgtcg gcataccctg ccgcatcgtc aacggcgtca
      481 ccctcaagga accctacgac gtcgaactga ccaacggcac cctcaccctg cgcatggccc
      541 agggtcgcca ttcctggatc gaggtctggt ttcccgatct gggctgggta ccatttgatc
      601 cgcagcagac tgccctctat gtcagcaacc gcttcattcg catcgagttc ggcctcgaca
      661 acgaggagac ctgcaacgac ggcctcattc gctggtccca ggtccagggc gctgagggca
      721 atccccaatt cgaggagacc atcaattatg tcctcgcctc ggaccgtgtc aacctgcgcg
      781 cggagaagca gaactacggt ccccagaagc tgctcttctt cccggccgtc gaagctgctt
      841 tcatcccggt ggccgcccgt tcgtccctgc ctccagctcc caaacccagg ctcactgaac
      901 agaagatcat gcgccgcatc gcctacagcc agcccttcag tcagggcaac actgaatttc
      961 cccagaatac cgactttctc tccgcacgcg gtccagcgcg cctggctgag agcggggaga
     1021 tggagatgcg caagaacttt atggtagaaa ccgcggagta tgtcaccacc cagggcaacc
     1081 agtatgccca gactttcatc ctcgaaaaac ccctcaagct cacccgcatc ggcctcgctt
     1141 tgcacaaatt cggcggttcg ggtcagcttt ggctcgagat tctccgtgat gacggcgaag
     1201 gcaagcccgg ggaatatatc gccaccagcc gctacctcgc ccttgaggag atcaaatacg
     1261 ccccgggtta cgactgggtc gattttgact ttggcgcaga cgagctgctg ctgcctccgg
     1321 gccgctactg gatggccctc ggcttcaccg gcagccctat catcaactgg tttttttcgt
     1381 acggcaagcc tgtggggccg gaagatggca cccgctataa aacccttttc gatgaaacct
     1441 ggagtcgaag cctcgcctat gaatttaatt accgcatcat cggtttgact ggggagtagg
     1501 accggtgaag cttatccctc attccacact gaaggcatct tggacgatga atgactgaaa
     1561 aatacatcct cacccatgac ctaggaacga gttccaacaa agccgttctc ttcactctct
     1621 acgggcgtct ggtcgccgag gcccggcacg attactcggt tcactaccct caacccagct
     1681 atgcagagca ggatcccttc gattggctcc atgccgtcta cgactccacc cataaggcaa
     1741 tggaaattgc cgggatctct cctgatcaga tcgccggcat cactttctcc tgccaaatgc
     1801 agaccttggt ggccgtcgat gcacagggca tgcccgtcat gcccgcgatt tcctggctcg
     1861 acacccgggg ccgcgaaatc ctgtatgacc ggctctggac cagaccgcgc atcatcggct
     1921 atcaacccct ccgcctgctg cgctttctgc gcgtcaccgg gggggcgccc ggccacaccg
     1981 gcaaggaccc gatcgccaag atcatctggc ttaaggagaa gaggcccgat ctctacgcca
     2041 aaaccgccaa gttcctcgat gtcaaggatt tcgtcatcta ccatctcacc ggcacctggt
     2101 gcaaatcggt cgatctggcc gtggtctggt ggctcctcga cacccgtaaa aacaggaacc
     2161 agtgggacga aaaactctgc cggttggctg gtatcgaccc tgagctgctg ccgcccgctc
     2221 tccccagcgc cgccatcgtc ggcaccctgc aggaagaggc cgcccgcctt atgggtctga
     2281 aaccggggat cccgatcatc aacggttccg gggatatccc cgccgccgcg gtcggctccg
     2341 gagccctcca tgaaggggaa ctcagcatcc gcctcggcac cagcggcggc atcgccggcc
     2401 atttcagccg ccgcaagatc gacctcgccc actacgctgg ctgcatcggc agcacctatc
     2461 cggaaaagta ttatctcgcc ctcgcccatc aggagacgat cggcatctgt ctcgaatggc
     2521 tggccaaccg catcctctat cacaaacagc gactctccga agagaccctt ttcgaagatg
     2581 tttaccagct cctcgaccac ctcgccgctc aggccccccc gggcgcagcc ggcctcatct
     2641 tcaccccctg gatgttcgga gaacgctgcc cgatcgacga ccacaacgtc cgcgccggcc
     2701 tcttcaacct cagcctccag cacggacgcg aacacctcat ccgcgccgtc ctggagggta
     2761 tcgccttcaa cctgcgctgg ggcctcgagg tcctggaaaa gctctatcaa cccgtcgacc
     2821 ggctccacat catcggcggc ggggccaaga gcgatatctg gtgccagatc atcgccgata
     2881 tcaccaaccg cacgatctgc caggccgaca atccccagca ggccaacgcc cgcggcgccg
     2941 ccatgctcgc cagcctttcc ctcggccata tcaagagctt cgaagcgatc agcgaacaca
     3001 tcgtcatccg ccagcgctat acaccggatc caaaaaaccg ccagctctac gaccatctct
     3061 tcagggagtt caaaaagctc taccggcaga acaagagctg gtacgcccgc atgaatggcg
     3121 aaaccggatg ggcactcaca aaccgcggcg tgcagcccta gccatgcccc tttttatacc
     3181 ggcgtccttt acggggagaa tctgagatgt cccagcgggc cacaggctcc attctgatcg
     3241 ttgatgatga ggccatcatc cgcgattcat tgcagcaatg gcttgaaatc gagggcttcc
     3301 gggtcttcag cgctgaggac ggcgagcggg cgttacaact ttgccgtttc gaacgccttg
     3361 atgtcggggt attcgacatc cgcatgcccg gaatggatgg catcaccctg ctcaaccgga
     3421 cccgcagctg ccgcccggag atggatgtca tcatgatgac cgccttcgcc agcatcgagg
     3481 atgccgtacg ctgcatcagt gagggggccc atgactatat catcaaaccc tttccgcctg
     3541 aaaaactcag caaatccatc cgtcatcttt tggaatcccg caagcttcac gcctcccaac
     3601 aagacctgat cagccagcaa ggggagctca gacgttttat cgcagcctgc accccctatc
     3661 tcgccctcgg ggcggctaca gccgtcctct ccggagcccc ctgggctgag caaaccggcg
     3721 gcggggaggc cgcgctggaa ccgtccctgc tcaaggagct gatcgatcgg gctctggaaa
     3781 tcagccgtcc ggcagagcaa accccctcca gcaacctgcg cagcgtggtc gagaccgccc
     3841 tgctgctggc tgagaccccg ccggagggcc gcgcccgact cagccccgca ccccccgcca
     3901 cagagatgag cgctggtatt ccctttgttc cggccatcct cgcccttcaa ctgctcttcg
     3961 agcaccttct ccggcacgga caccccgacc ccattatcaa ccttgaggcg gaaaagagcg
     4021 caccactacg ccctgtcctg cgaattggac tggccgcacc catgtccgtc aaagatcggg
     4081 cgcagctgct ggagcagcag gagggaggcg cctccgattc gagccgactc cggctcgctg
     4141 caaccctgct gcggattctg ggcgtagccg tgacggtaac tccgcgccgg gaggagggcg
     4201 aaaccatcct cctcatcttc ccctccgctg aggttaacag gtagatcgcg atgacaaacg
     4261 aagtaaaaat cctcattgtc gacgatgaag agctcttgcg tgaaggctgc cgccgcttgc
     4321 ttgaaatgtc cggctacacg gtgaggacgg cgggggaggc cgattcgggg ctgagattgg
     4381 cggccgcaga tgctttcgag atcgctttcg tcgattttcg catgccgggg atgagcggga
     4441 tcgatttcat ccgccgcatg caggagatca gcccggcgac cgacgtagta atgatgaccg
     4501 gttatgcgag catcgagatg gccgtggagg cgatgaagaa cggcgctcgc gattatgtcg
     4561 ccaaaccctt cgaggccgag cagatcctcg agattgtccg caagcttttg cagaaccgtc
     4621 agacccccac tgcgcccgcc ggcgcaggct tcagctttga tttcaatggc cggccgctgc
     4681 agattatcgg cggaggcggc cgcatgcagg aacttttcgc cctcatccgc aaggtcgctc
     4741 cgaccgagag caccgtcctg gtccaggggg agagcggcac cggcaaggaa ctggtcgcca
     4801 aggccatcca cgctctcagt ccccgcaaag acaaggcctt ttttgcaatg gattgcggtt
     4861 cgctggtcga atccctcttt gagagcgagc ttttcggaca cgtcaagggc tccttcacag
     4921 gagccaccgc caccaagcat ggcgccttcg aactcgctca c
//
LOCUS       DAOPWU010000267         4884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_17407_length_4884_cov_4.635742, whole genome shotgun sequence.
ACCESSION   DAOPWU010000267 DAOPWU010000000
VERSION     DAOPWU010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17407_length_4884_cov_4.635742"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1061
                     /locus_tag="PKM23_11865"
     CDS             222..1061
                     /locus_tag="PKM23_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003551420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNY92210.1"
                     /translation="MDSLKNCVALVTGASRGVGKGVALGLGEARATVYVTGRTVIPAT
                     GPMDLPGTIGETADEVSRLGGMGIPVRCDHTDEHQVEELFARIDTEQKKLDVLVNCVW
                     GGYERMFDNNEFTWPYPFWMQPLWRWDAMFNAGLRAHYVASTLAAKRMVAQRSGLIIN
                     ISFWAAQKRIGNVAYGVSKAATDKMTADMAEELEPHGVAVICLYPGLVRTESVMRAAE
                     FLDLSNSESPQFIGRAVAALASDPDVIKRTGTVCVAAALAKEYGFADIDGKQPVPLSI
                     KDV"
     gene            complement(1127..3202)
                     /locus_tag="PKM23_11870"
     CDS             complement(1127..3202)
                     /locus_tag="PKM23_11870"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNY92211.1"
                     /translation="MARPFHHPVFSGLLAVCVALSASAAAVGGGSVGTFTLGGTTGVY
                     VATVDSLLAYNQPSHEKSSDYLWASDQVVAISLNGTTIAATGSGVTIDGTRATITKGG
                     TYSLSGALADGQIVVSAGANDIVRLILNGVTLANSTNAPINIQSADKAIVILAENSVN
                     TLTDPSVYVFPDAITDEPNAALYSKTDLTIAGAGSLVVNANFNDGITSKDGLVIAGGT
                     ITVKSVDDGIRGKDYLIIRDGTLTVDAKGDGFKADNDEDADRGYIWISGGAIRITAGD
                     DAMMAETDLLISDGTITATTGGGSTYAPTSTTDPKGLVATLFNVIDGGAITINAADDA
                     LHSNGHLVINSGTLVLSSGDDGIHADSTLGINGGEITISKCYEGIESKMVAINDGRIH
                     LVSSDDGVNGAGGKDGSGKPGWPGGPPMASGNYFLYLNGGYIVINANGDGIDVNGTIT
                     MTGGTVIVHGPTNDGNGALDYDRSFTMTGGLLVAAGSAGMAQAPGSSSTQNSILVNFS
                     SRNTGKLFHVAGPSGENLLTFLPAKSWQSVVFSSPKLVRGANYVVHYGGSASGTAADG
                     LYSDGSYTPGTTYAKFTISSVVTKISRSTGLEEGESTPVNFTLFNAYPNPFNPRTTVR
                     YQLGQTVPIRLSIFNLLGEEVACLTDGSEEAGLHERVWDAAGLAGGLYLVRLTAGEER
                     ATQRIMLVK"
     gene            3461..4054
                     /locus_tag="PKM23_11875"
     CDS             3461..4054
                     /locus_tag="PKM23_11875"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNY92212.1"
                     /translation="MQHMTGWPVQTDQELIDRFQQGDESTFDELVRRHEQRIRALCGC
                     YLSDPQDVQDAAQETFIRAFRGLPSFRPEARFSTWLFRIAIHTSLNQVRSRRRRGWMQ
                     AFSRLPGMEDQIVTSRMSAPTPLEEVERAEQQRLIRAALDSLPEEQRTAVILHRFHGL
                     KYQEIADATGSSLAAVEARIHRAKGRLAVLLADYMKE"
     gene            4147..4599
                     /locus_tag="PKM23_11880"
     CDS             4147..4599
                     /locus_tag="PKM23_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNY92213.1"
                     /translation="MRRAMTCKEIEKRLSAHLDGELEMQSSHAVQEHIAGCPACRRAA
                     ARLQQVYALLPEVEIPAPDPFMATRVRAALAGKRRFRPVHRLLLPASVAAGLVLGMIL
                     GRDLYTRWSGSSTVTMENLEVRMLQKVPGSSLTASYLDLSNPEGNSYE"
     gene            4592..>4884
                     /locus_tag="PKM23_11885"
     CDS             4592..>4884
                     /locus_tag="PKM23_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92214.1"
                     /translation="MNKWLVALLVFSLAVNLAVVGTLIYFTQRPEMAHRGAGGPVAPP
                     GAPQGMPPRPGDEGVWPGGRTEPNPKMEKLRLAYHEQLQPLHGSMLQLQQDLIR"
BASE COUNT          989 a         1377 c         1524 g          994 t
ORIGIN      
        1 aaggaagcag ataaagaaac gtatgtgatc gtccgttaaa gatggttttg atgatgcaac
       61 cggcattggc tttcccccaa aaaggcggcc caacaagcga ctgcatgcga atgcacaggc
      121 aagttttttc ttgaagcaag gttttatact tggagtagaa tgtataccct aaattcgtcg
      181 gcagcgtgcg agctgtaggc gttcggcacg aaaggaaaaa tatggatagt ttgaaaaact
      241 gtgttgctct tgtgacgggt gcaagcaggg gagtcggaaa gggtgtggcg ctcggattgg
      301 gagaagccag ggcaacagtt tatgtaaccg ggcgaaccgt aataccggca accgggccga
      361 tggacttacc cggcaccatc ggggagacgg ccgatgaggt ctcccgcctg ggaggtatgg
      421 gcatccccgt tcgctgcgat cataccgatg aacatcaggt tgaggaactg ttcgcccgga
      481 ttgacacgga gcaaaagaag ctcgacgtgc tggtcaactg cgtctggggt ggttatgagc
      541 gcatgtttga caacaatgaa tttacctggc cctatccttt ctggatgcag cctctctggc
      601 gatgggacgc gatgttcaac gccggcctgc gcgcgcacta tgtggccagc acattggccg
      661 ccaaacgcat ggttgcacaa agatcgggac tgatcatcaa tatctccttc tgggctgccc
      721 agaaaagaat cggcaacgta gcttacggag tgtccaaagc tgcaacggac aagatgaccg
      781 cagacatggc cgaggaattg gaaccacacg gtgttgcggt gatttgcctt tatcccggac
      841 tggtcaggac cgaaagcgtc atgcgtgcgg ctgaatttct cgacttgagc aattccgaat
      901 cgccgcagtt catcggccgt gccgttgcag ccctcgcctc cgatcccgac gtaatcaagc
      961 gcaccggcac ggtctgtgtg gctgcggccc ttgcgaaaga atacggattc gctgatatcg
     1021 acggcaaaca gccggttccc ttgtccatca aggatgtatg aaccaggccg aaccagggag
     1081 tccaggcgga gggttccgcc ttccctgacc gggcgtggca gaatccctac ttgaccagca
     1141 tgatcctctg cgtcgcccgc tcctcccccg cggtcagccg caccagatag aggccaccgg
     1201 ccaagccggc cgcgtcccaa acccgctcgt gaagccccgc ctcctcgctc ccatccgtca
     1261 ggcacgcaac ctcctcacct aaaagattaa agatcgagag tcgaattggg acggtctgac
     1321 ccagctggta gcggaccgtg gtgcgcggat tgaagggatt cggataggcg ttgaagaggg
     1381 tgaaattcac tggagtggac tccccctcct ccaggccggt cgaacgggaa attttggtca
     1441 ccaccgagct gatggtgaat ttggcgtagg tggtgcccgg ggtatagctg ccatcgctgt
     1501 agaggccgtc cgcggcagtt ccggaggcgc tgccgccgta atggacgaca tagttcgccc
     1561 cgcgcacgag ttttggcgag gagaagacca cagactgcca gctcttggcg gggagaaagg
     1621 tgaggaggtt ctctccggag gggccggcga catgaaagag cttgccggta ttgcgcgaag
     1681 aaaaattgac cagaatggag ttctgcgtcg agctgctgcc gggggcctgg gccattccgg
     1741 cgctgcccgc ggcgacgagc aagccgccgg tcatggtgaa gctgcggtcg tagtccagcg
     1801 cgccgttgcc gtcattggtg gggccgtgga cgatgacggt gccgccggtc atggtgatcg
     1861 tgccattgac atcgatgccg tcgccgttgg cattgatgac gatgtaaccg ccgttgagat
     1921 agagaaaata gttgccggag gccatcggcg gtccccccgg ccagcccggt ttgccggagc
     1981 catccttgcc gccagcgccg ttgacgccgt cgtcgctgga gacgagatgg atgcgtccgt
     2041 cattgatggc gaccatcttg ctctcgatgc cttcataaca tttactgatg gtgatctctc
     2101 cgccgttgat gcccagggtg gaatcggcat gaataccatc gtctccgctg gagagaacca
     2161 gtgtgccgct gttgatgacc aggtgcccgt tggaatggag ggcatcatcc gctgcgttga
     2221 tagtgatggc gccgccatcg ataacgttga agagggttgc caccagcccc ttgggatcgg
     2281 ttgtggaggt cggggcatag gtactgccgc cgcctgttgt ggcggtgatg gtgccatcgc
     2341 tgatgagcag gtcggtttcg gccatcatgg catcatcgcc ggcggtgatc cggatcgcac
     2401 cgccgctgat ccagatatag ccgcggtcgg catcctcgtc attgtcagcc ttgaaaccgt
     2461 ccccctttgc atccaccgtg agggtcccat ccctgataat gagatagtcc ttgccgcgga
     2521 tgccgtcatc cacggatttg acggtgatcg ttccgccggc gatgaccagg ccatccttgc
     2581 tggtgatgcc atcgttgaaa ttggcgttga ccaccagcga gcccgcaccg gcgatagtca
     2641 ggtcggtttt gctgtagagg gcggcattgg gttcgtccgt gatcgcatcg ggaaaaacgt
     2701 aaacggaggg atctgtgagg gtattgacgc tattttccgc cagaatgacg atggccttgt
     2761 ccgcgctctg gatgttgatc ggggcgttgg tggaattggc gagggtcacc ccgttgagga
     2821 tgaggcggac gatatcgttc gcccctgcac tgaccacgat ctggccgtca gcgagggcgc
     2881 cgctcaggct gtaggtgccg cctttcgtga tcgtggcccg ggtgccgtcg atggtgacgc
     2941 cgctgcctgt ggccgctatc gttgtcccgt tcaggctgat cgcgacgacc tgatcgctcg
     3001 cccagagata atcggagctt ttctcgtggg agggttggtt ataggccagc agcgagtcca
     3061 ctgtagcgac atagacgccc gtcgtgccgc ccagggtaaa agtgccaacg cttccgccgc
     3121 ccaccgcagc tgcagaagcg gacagggcca cacacacggc caggaggccg gaaaagaccg
     3181 ggtgatggaa tggtcgagcc attttctctc ctctttttcc ttttgaacac ctcaggagga
     3241 gaaaacttgc gtggaaaaat cactattttc acaggcctgc cggcacggcg ggtgaggtgt
     3301 tcctgccatc tcagtctttc gggattattt catccagcag cagtacggca cctcatcgtt
     3361 cacggcggga aaggtgctcc accaaagagt atgcaataaa atggcggttg tggtgtttaa
     3421 ataagaaaca agccggaaac agcgtatgtc aaatcagcca atgcagcaca tgacaggatg
     3481 gccggtgcaa acggaccagg aattgatcga ccgttttcag cagggcgatg aaagcacatt
     3541 tgacgagctc gtgcgccgtc atgaacagcg catccgcgcc ctgtgcggct gctatctctc
     3601 cgatccgcag gacgttcagg atgccgcgca ggagaccttt atccgtgcct ttcgcggtct
     3661 gccctctttc cggccggaag cccgcttttc cacctggttg ttccgcatcg ccatccatac
     3721 cagtctgaat caggtgcgca gccggcgccg gcggggctgg atgcaagctt tctcccggct
     3781 gcccggcatg gaggatcaga tcgtgaccag caggatgagc gctcctacac ccctggagga
     3841 ggtcgagcgg gcagaacagc agcgcctgat ccgcgccgcc ctcgattccc tgccggaaga
     3901 gcagcgtaca gcggttatcc tccatcgctt tcatggtctg aaataccagg agatcgccga
     3961 cgccaccggt agcagcctcg ccgcggtaga agcgcgcatc caccgcgcca aaggcaggct
     4021 ggccgtgctg cttgcggact atatgaagga gtgagcctcc ggacgggagc ggccgcgaaa
     4081 atgcccttac tgtggcaaga attttcgggt tgcggtgttg aattgatgag aagagcaggt
     4141 ctatttatga gaagagccat gacgtgcaag gagatcgaaa aacgcctctc cgcccatctc
     4201 gatggagagc tggagatgca gtccagccac gcggtgcaag agcatatcgc cggctgcccg
     4261 gcgtgccggc gtgccgctgc gcggctgcag caggtctatg ccctgctgcc ggaggtggaa
     4321 atcccggcgc cggatccttt catggccacg cgggtgcgcg cggccctggc cggcaagcgc
     4381 agattccggc cagttcaccg cctgctgctt ccggcctctg tagctgccgg acttgtcctg
     4441 ggaatgattt taggcaggga tctctacacg cgttggtccg gcagcagcac tgtgaccatg
     4501 gagaacctcg aggttcgcat gctgcaaaag gttcccggca gctccctgac cgccagttac
     4561 ctggatctct caaatcctga agggaattcc tatgaataaa tggctagtcg cactgctcgt
     4621 attttccctc gccgtcaatc tggctgtggt gggcacgctg atctatttca cacagcgacc
     4681 ggagatggcg cacagaggcg ccggggggcc tgtcgcaccg ccgggggcgc cgcagggcat
     4741 gccgccccga ccgggtgatg agggggtatg gccgggaggg cggactgagc cgaatccgaa
     4801 gatggaaaaa ctgcgattgg cctatcatga acagctgcag cccttgcatg gcagcatgct
     4861 gcaactccaa caggatctca tccg
//
LOCUS       DAOPWU010000268         4870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_17479_length_4870_cov_3.860021, whole genome shotgun sequence.
ACCESSION   DAOPWU010000268 DAOPWU010000000
VERSION     DAOPWU010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17479_length_4870_cov_3.860021"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PKM23_11890"
     CDS             <1..1005
                     /locus_tag="PKM23_11890"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNY92215.1"
                     /translation="ELAAIQTPQDWAVLKIGAAIPGESRTAARLIQPRMAIITNVGEA
                     HLDHYEGIEAIADAKLELLNALSPSGIAILNRDNEYTRLMGLKLAGKVLYYGLSPVSD
                     YFADSIQHLGPEGTAFTIHSRGGEKVSIRMALYSLGDVYNALAAYAAAAELGIPPGII
                     KNSLETGFTLPEGRGRLHSFPGLHILDDTHDATPQAFYKSTKSLINFRRHAKRLIFVM
                     GKMTEIGGQTRVMHTMMGHYLAGMPIDVAVLVGEYASAAASAIAAATGRRKRVVTCTT
                     VEEACTWLRQNLKAGDVVLVEGGETNDMAAIVRDLVRFGSESGGRSISTNPRINSIVQ
                     "
     gene            1064..1360
                     /locus_tag="PKM23_11895"
     CDS             1064..1360
                     /locus_tag="PKM23_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HNY92216.1"
                     /translation="MASSRERDVSSVCISDILSDVMAKVPRDAIWITNQAHENVIAIA
                     FFKELTAVIFTNGAVPAMDAIDKANIKKIALYSCDLNAFDIAGRLYTLGLRGSY"
     gene            1391..2152
                     /locus_tag="PKM23_11900"
     CDS             1391..2152
                     /locus_tag="PKM23_11900"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HNY92217.1"
                     /translation="MHTVLSPCSELTMGPRDIVQTAIAKGLDFIAITDHNSGENVTAV
                     QGAAQGTPLQVFPGIEVSTREEIHMITLFPSLERLFGFQKWLYGQLQEGENDPTLWGP
                     QIVVDAQENIVGENNRLLGLPIRQSYDQVVREAVAREGIIYPAHIDRRANSMVRTLGF
                     LPADLPFRVVELSRRANLADAVERYSSSGLQLITASDAHDIDQLGSARSWFKMAGTTF
                     PELLQACRGENGRCIAVLQPDEAGRSGAPCAAAAH"
     gene            2171..2719
                     /locus_tag="PKM23_11905"
     CDS             2171..2719
                     /locus_tag="PKM23_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY92218.1"
                     /translation="MRELSLHILDIVTNSIEAEASRVILWLEESVEKNLFSITVRDNG
                     RGMSQEMIDAVIDPFVTTRTTRPVGMGLPLLRQAATSCGGELRIKSRIGKGTTLTASF
                     LHNSLNRSPLGGIAETVVNLIIGAPDVHFLYGHRTDAGCFTFDSYWIYARMAELDCSQ
                     YMLVEPAQKWIMERLQSIASTA"
     gene            2846..3211
                     /locus_tag="PKM23_11910"
     CDS             2846..3211
                     /locus_tag="PKM23_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNY92219.1"
                     /translation="MKSLSDLRAIKEKAKKEMAAREQSAKYRIVIAMGTCGIAAGARA
                     VMSAILDEINKRNVADVVVTQTGCLGFCDQEPLVQVVQTDKGVTTTYGKINPEMARKI
                     IDEHVLGDHIVSSHVFSHE"
     gene            3277..3753
                     /gene="nuoE"
                     /locus_tag="PKM23_11915"
     CDS             3277..3753
                     /gene="nuoE"
                     /locus_tag="PKM23_11915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNY92220.1"
                     /translation="MTDYTAVDRIIKSYETTKTPLIYILKDVQKAYGYLSEDILTRIA
                     KKVDIPLSEIYGVATFYSLFTTKPKGKHIVRCCNNAPCYVKDSKEVLAQIKSYLGIET
                     GETTPDGLFTLEFTSCLGLCAVAPVMMIDSEVYGNLTPEKAIAILKDYKVKGSSNA"
     gene            3746..>4870
                     /locus_tag="PKM23_11920"
     CDS             3746..>4870
                     /locus_tag="PKM23_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92221.1"
                     /translation="MHRIHILVAMDAHTISQGARSVKDALVKEVALAGLADDVRVVET
                     GSLGIYDKGVVLVIFPDAVYYVGVKMEDVSEIIAEHLLKGRIVERLHYLDIPSAEGEM
                     GEKSARICKQERVVLRNAGVIDPESIEEYIAHDGYEALGKALTEMTPEAVMEEVKKSG
                     MQGRGGAFFPTGLKWSFAAKEKSEEKYIICNADEGEPGTFKDRLILEGDPHAVIEGMI
                     LAGFAVGAHQGYVYIRGEYHMSIARVKRAIEQARAHGLLGKSIFGTPFSFDLEIREGA
                     GAYICGEETALIESIEGKRGEPRNKPPFPPSVGLWGKPTIVNNVETLANVPQIIRKGA
                     EWYKGIGTAKSPGTKVFTMVGNINNPGLIEVPMGITLREII"
BASE COUNT         1097 a         1528 c         1315 g          930 t
ORIGIN      
        1 gaacttgccg ccatccagac cccacaggat tgggccgtgc tgaaaatcgg cgccgccatc
       61 cctggtgaaa gccgcaccgc cgcacgcttg attcaacccc gcatggcgat cataaccaac
      121 gtcggcgaag cccacctcga ccactatgaa ggcatcgagg ccatcgccga cgccaagctc
      181 gaactcctca acgccctctc acccagcggc atcgccatcc taaaccgcga caatgagtat
      241 acccgcctca tgggactcaa actggccgga aaagtgctct attacggcct gagtccggtc
      301 tcggactatt tcgccgattc gatccagcat ctcggaccgg agggcactgc cttcaccatt
      361 cacagccgag gcggggaaaa ggtgagcatc aggatggccc tctactccct cggagatgtc
      421 tataacgccc tcgctgccta cgccgcagcc gccgagcttg gcataccacc cggcatcatc
      481 aaaaacagcc tcgagaccgg ttttaccctt ccagaaggcc gcggccgtct gcactccttc
      541 cccggcctgc acatcctcga tgatacccac gacgctacac cccaggcgtt ctataaatcg
      601 accaaatccc tgatcaactt ccgccgccac gctaaacggc tcatcttcgt catgggcaag
      661 atgaccgaaa tcggcggcca gacccgggtg atgcacacca tgatgggcca ctacctcgcc
      721 ggcatgccca tcgacgtcgc cgtgctggtc ggtgaatacg ccagcgctgc cgcctccgcc
      781 atcgccgccg ctacaggccg gcgaaaacgg gtcgtcacct gcacaacggt cgaggaggcg
      841 tgcacctggc tgcggcagaa cctcaaagcc ggggacgtcg tcctcgtgga aggaggggag
      901 accaacgaca tggctgccat tgttcgcgac cttgtccgct tcggcagtga atcagggggg
      961 cgctcaatat ccactaatcc acgtatcaat tcgatagtac agtaaagacg aaaccatgga
     1021 atttacaaaa atacttgaag aaataccttc aaactgcctt acaatggcta gttccagaga
     1081 acgagatgta tcatcggtct gcatctcgga catcctcagc gatgtcatgg ccaaggtgcc
     1141 tcgggacgca atctggatca ccaatcaggc ccatgaaaat gtcatcgcta tcgccttttt
     1201 caaggagctg acggcagtca ttttcaccaa cggcgccgtg cccgccatgg atgcgatcga
     1261 caaggccaat atcaaaaaga tcgccctgta cagctgcgac ctgaacgcct tcgatatcgc
     1321 gggccgactc tataccctgg gtctgcgcgg gagctactga atgctgcgct ggtattgcgg
     1381 agacctccat atgcataccg tgctgtcacc ctgctcggaa ttgaccatgg ggccccggga
     1441 catcgttcag accgccatcg cgaagggact cgatttcatc gcgatcaccg atcataattc
     1501 gggggaaaac gtcaccgctg tgcagggagc cgcccagggc actcctctgc aggtctttcc
     1561 aggcatcgag gtttccactc gggaagagat ccatatgatc actctcttcc cctccctgga
     1621 acgactgttc ggctttcaga aatggctcta cggtcagctg caggaggggg agaacgaccc
     1681 cactttgtgg ggaccgcaga tcgtcgtcga tgcgcaggaa aacatcgttg gggaaaacaa
     1741 tcgactgctc ggtctgccca tacggcagtc ctatgatcag gtcgtgcgcg aggcggtcgc
     1801 ccgggagggg atcatctatc ccgcccatat cgaccggcgc gccaacagca tggtgcgcac
     1861 cctcggcttt ctgcctgccg atctgccctt ccgggtcgtt gaactctctc gccgcgccaa
     1921 cctggccgac gcggtggagc gttacagcag cagcggtctg cagctgatca ccgcctcaga
     1981 cgcccatgat atcgatcaac tcggctctgc ccggagctgg ttcaaaatgg ccggaaccac
     2041 tttccccgag ctgctccagg cctgccgggg ggagaacggc cgctgcatcg ctgtgctcca
     2101 gcccgacgaa gccggacgca gcggggcgcc ctgcgcagcc gccgcgcact gaccaggaaa
     2161 aaggacgact gtgcgcgaac tctcactgca tattctggat attgtcacca attcgatcga
     2221 ggcggaagcc agtcgcgtca tcctctggct cgaggaatca gtggagaaaa atcttttttc
     2281 catcaccgtg cgcgacaacg gacgcggcat gagccaggag atgatcgatg cggtcatcga
     2341 tcccttcgtc acgaccagga ccacccgtcc ggtcggaatg ggtctgcccc tgctcaggca
     2401 ggcggcgacc agctgcgggg gagagctgcg catcaaatcc aggatcggca agggcactac
     2461 cttgaccgct tcgtttctgc acaacagcct gaaccgttcc cccctcggcg gcatcgccga
     2521 gaccgtggtc aatctcatca tcggtgcgcc tgatgtccat tttctctacg gccatcgcac
     2581 cgacgcaggc tgctttacct ttgattcata ctggatttac gcccggatgg cggaactgga
     2641 ttgttcccaa tacatgctgg ttgagccggc gcaaaaatgg atcatggagc ggctgcagtc
     2701 gatcgcttcg accgcctgat ccacccagtg atcccgcgcc ggcccgatgc cggagtttta
     2761 gaagctctac ttaatcaaca gatccacatc ggcgaaggct ttttccaggt ccgccggtga
     2821 aacacaactg caggaggaat accgcgtgaa aagtttgtca gatctccgcg ccatcaagga
     2881 aaaagccaag aaggaaatgg ccgcccgcga acaatcggcc aaataccgca tcgtcatcgc
     2941 catggggacc tgcggtatcg ctgccggcgc ccgcgctgtc atgtccgcta tcctcgatga
     3001 aataaacaag agaaacgtcg ccgacgtcgt cgtcacccag accggctgcc tcggtttctg
     3061 tgatcaggaa cccctcgtac aggtggtgca gaccgacaag ggcgtcacca ccacttacgg
     3121 caagatcaat ccggaaatgg cccgcaaaat aatcgatgag catgtcctgg gagatcacat
     3181 cgtctccagc catgtcttct cgcatgaata atggccggca actccactcc gagttgaacc
     3241 gttgtcccta acttgccgcg ttttatggag gaacccatga cggattacac tgcagtagat
     3301 agaattatca aatcctatga aacgaccaaa acgccgctca tctatatcct caaggatgtg
     3361 caaaaggcct atggttatct ctccgaggac attctcaccc gcatcgccaa aaaggtcgat
     3421 atccccctca gtgaaatcta tggcgtcgcc actttctact ctcttttcac caccaagccc
     3481 aagggcaaac acatcgtgcg ctgctgcaac aatgccccct gctatgtcaa ggattccaag
     3541 gaggttctcg cccagatcaa gagctatctc ggcatcgaga ccggcgagac caccccggac
     3601 ggactcttca ccctcgagtt taccagctgc ctcgggctct gtgctgtcgc cccggtcatg
     3661 atgatcgaca gtgaggttta tggcaatctc actcccgaga aagcgatcgc gatattgaaa
     3721 gactacaaag tgaaaggatc ctccaatgca tagaatccat attctggtcg caatggatgc
     3781 ccataccata tcccagggtg cccgctcggt caaagacgcc ctggtcaagg aagtagccct
     3841 ggccggactg gccgatgatg tgcgcgtggt cgaaacgggc agcctcggca tctacgacaa
     3901 gggcgttgtg ctggttattt ttcctgatgc cgtctactac gtcggcgtca agatggagga
     3961 tgtctccgag atcatcgccg agcatctgct caagggccgc attgtcgaac gcctgcacta
     4021 tctcgatatt ccgtcggctg agggcgagat gggggaaaag tcggcgcgca tttgtaaaca
     4081 ggagcgcgtg gtgctcagaa acgccggcgt gatcgatcct gaaagcatcg aggaatacat
     4141 cgcccatgac ggctacgagg ccctcggcaa ggccctcacg gagatgaccc cggaagcggt
     4201 catggaggag gtgaaaaagt cggggatgca gggccgcggc ggcgccttct tcccgaccgg
     4261 cctcaaatgg agctttgcgg ccaaggaaaa atcggaagaa aaatatatca tttgcaacgc
     4321 cgatgagggc gagcccggca ccttcaagga ccgcctcatc cttgaaggcg atccccacgc
     4381 cgtcatcgaa gggatgatcc tggccggctt tgccgtcggg gcccatcagg gctacgtcta
     4441 catccgcgga gaatatcaca tgtccatcgc ccgggtcaag cgcgccatcg aacaggcccg
     4501 cgcccacggc ctgctcggca agagcatttt cggcaccccc ttctccttcg atctcgagat
     4561 ccgcgaaggg gccggggcct atatctgcgg cgaggaaacc gctttgatcg aatcgatcga
     4621 aggcaagcgc ggggagccgc gcaacaagcc gcccttcccg ccctccgtag gcctctgggg
     4681 aaagccgacg attgtcaaca atgtcgagac cctcgccaat gtgccccaga tcatccgcaa
     4741 gggggcggag tggtacaagg gcatcggcac ggccaagagt cccggcacca aggtctttac
     4801 gatggtcggc aatattaaca atcccggcct catcgaggtg ccgatgggca tcaccctgcg
     4861 tgaaatcatc
//
LOCUS       DAOPWU010000269         4833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_17624_length_4833_cov_4.912097, whole genome shotgun sequence.
ACCESSION   DAOPWU010000269 DAOPWU010000000
VERSION     DAOPWU010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17624_length_4833_cov_4.912097"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /gene="hisD"
                     /locus_tag="PKM23_11925"
     CDS             complement(<1..820)
                     /gene="hisD"
                     /locus_tag="PKM23_11925"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNY92222.1"
                     /translation="MISIYRNGAVEEFIARYESRRTQTAGDVEARVAGIIADVRERGD
                     EAVLEYARRFDGAVLTPATLAVPAGEMERAHTECDGALLEVIREAIGNIMAFHRKSVP
                     RSWLDWEGDGVVLGQRAIPLERVGLYVPGGRAAYPSSLIMAAAPAIAAGVSEIVVVTP
                     CGADLQVNPVILATAFALGITRLFRVGGSQAIAALAFGTESIPRVDKIAGPGNIYVAT
                     AKKLLFGQCGIDMVAGPSEVLIVADESAEPGFVAADLLAQAEHDPLASSLLVTDS"
     gene            complement(865..1761)
                     /gene="hisG"
                     /locus_tag="PKM23_11930"
     CDS             complement(865..1761)
                     /gene="hisG"
                     /locus_tag="PKM23_11930"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011746130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNY92223.1"
                     /translation="MVDWNGLDERVLRIGLPKGSLQEATFRLLAKAGYHFSVSERSYF
                     PSIDDEEIEAILIRAQEIARYVQEGVFDVGLTGKDWIMECRADVVEVAELTYAKLTMR
                     PVRWVLAVPADSPVQSVRDLQGKRIATELVNMTRDYLERHQVQAEVEFSWGATEVKAP
                     ALVDAIVEVTETGSSLRANNLRIIETLLESRTMLIANRKSWADPAKREKIENFNMMLQ
                     GAIRAEGKVGLKMNAPAARLEELMKELPAMKNPTISPLHGSEWVAVDTIIEEQSVRKL
                     IPRLKKAGAQDIIEYPLNKVIY"
     gene            complement(1959..2639)
                     /locus_tag="PKM23_11935"
     CDS             complement(1959..2639)
                     /locus_tag="PKM23_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNY92224.1"
                     /translation="MKLLLFDIDGTLVHSGGAGLRAMTRAFTDLYAIPDALAGVSLSG
                     RTDSSILQDALAIFALPHSPEIEEEYKQRYFELLAEEMPLPGAGKRLMPGIPALLEAL
                     SGRPDVHLGLLTGNWRTSGYLKLADFGLDRYFPFGAFADDSGVRDQLLPFAVARFRDL
                     SGLSPRPEEVYVIGDTPYDIQCARPHGAVAVAVAAAAHSRSHLAACNPDHLFTDLEDT
                     EAALRVLG"
     gene            complement(2639..3778)
                     /locus_tag="PKM23_11940"
     CDS             complement(2639..3778)
                     /locus_tag="PKM23_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNY92225.1"
                     /translation="MSRKEFIHKTGKGIIAAGAASAASPLLAGCQKSSRSSSGGFRME
                     IKSKRLDLRHAWTIARNTSTYKEYAFVKLEKNGIYGLGEAAHNVRYGESLESIIAALE
                     KARPILENCDPWKFVDVNAAVQAVCEGQTAAKAAIDIALMDWISKSLGIPLYQFWGLD
                     PQKAPQTTFSIGIDTMEMVKQKVEEAAPYPLLKIKMGKDNDEEILAAVRSVTKKTLRV
                     DANEGWKEKELALRKVEWLAANGVDIVEQPMPSHMLEETAWVKERASIPILADESVKT
                     SADIPRLAEAFDGINIKVDKAGGLQESLRMIWMARSLGLKIMLGCMVSSSLSITAAAH
                     LSPLTDYPDLDGNLLLARDPFTGVTVRDGWLILPDGPGLGVSGEF"
     gene            3977..>4833
                     /locus_tag="PKM23_11945"
     CDS             3977..>4833
                     /locus_tag="PKM23_11945"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNY92226.1"
                     /translation="MSGQGSTVDPQLRRVLRDDLRRGGYAAQIRRENAEMKQFYLSDE
                     QRLRIRSMGTVRRFFAVTFWLLRSLLLHMSSFRRLMLILALLVIYSPRCEWHGTSFQA
                     SSDISLSGVLLLLFIIMLEVKDKMLARSELQAGRIVQKALIPNQPPAIPGWSIWLYYH
                     PANEVGGDLLDYLPINSHRHGFALGDISDKGLGAALLMAKLQATLRALAPECSDPADL
                     GQKMNRIYIRDTMANRFASMIYFEIASNESNIRFINAGHQPPLLVQGDQVSEWPKGGP
                     ALGIQPNAS"
BASE COUNT          886 a         1507 c         1532 g          908 t
ORIGIN      
        1 ccgaatcggt gaccagcagg ctggaggcga gggggtcatg ctccgcctgg gcgagcagat
       61 cggcggcgac aaaaccgggc tcggcgctct cgtcggctac gatcaggacc tcgctcgggc
      121 ccgccaccat gtcgatgccg cattggccga agagcagctt tttcgccgtc gccacataga
      181 tgttgccggg gccggcgatc ttgtccaccc gcggaatgct ctccgtgccg aaggcgaggg
      241 cggcgatggc ctggctgccc ccgacgcgaa agaggcgggt gatgcccaga gcaaaggcgg
      301 tggcgaggat gaccggattg acctgcaggt cggccccgca gggggtgacg acgacgatct
      361 cgctcacccc ggctgcaatg gccggggccg ccgccatgat cagggagctg ggataggcgg
      421 cgcgcccgcc cggcacgtag aggccgaccc gctcgagagg gatcgcccgc tggccgagca
      481 ctacgccatc cccctcccag tcgagccagg agcgggggac ggatttgcga tgaaaggcca
      541 tgatattgcc gatcgcctcg cgaatcacct ccagcagagc gccgtcgcac tccgtatggg
      601 cgcgttccat ctccccggcc gggacggcaa gggtggcggg agtcagaacg gcgccgtcga
      661 agcggcgggc atattcgagc acagcctcat ccccccgctc ccgcacatcc gcgatgatcc
      721 cggcgacgcg cgcctccaca tctccggccg tctgcgtccg ccgcgattcg tagcgggcga
      781 tgaactcctc caccgctccg tttctgtaga tgctgatcat gaaatgctcc attctttcag
      841 gtgcaccgcc tgcgcgggac cttgtcaata gatgaccttg ttgaggggat actcgataat
      901 gtcctgcgcg ccggcttttt tcagcctggg gatgagcttg cgcaccgact gctcctcgat
      961 gatggtatca accgccaccc attccgagcc gtggaggggg gagatggtcg gattcttcat
     1021 ggcgggcagc tctttcatca gctcctccag ccgcgcggcg ggggcgttca tcttgaggcc
     1081 gaccttgccc tcggcgcgga tcgccccctg gagcatcatg ttgaaattct cgatcttttc
     1141 ccgtttggca gggtcagccc agctcttgcg gttggcgatg agcatggtgc gggattcgag
     1201 cagggtctcg atgatgcgca gattgttggc gcgcagggag ctgccggtct cggtcacctc
     1261 aacgatggca tcgaccaggg ccggggcctt gacctcggtc gccccccagc tgaactcgac
     1321 ctcggcctgg acctggtggc gctccagata atccctggtc atgttgacca gctccgtggc
     1381 gatgcgcttg ccctgcaggt cccgaacgct ctggaccggg gaatccgccg gaaccgccag
     1441 cacccagcgc accggccgca tcgtcagctt ggcgtaggtc agctcggcca cctcgaccac
     1501 atcggcccgg cactccatga tccagtcctt gccggtcagg ccgacatcga aaaccccctc
     1561 ctgcacgtag cgggcgatct cctgggcgcg aatgaggatg gcctcgatct cctcatcatc
     1621 gatggagggg aaataggagc gttcgctgac cgaaaaatgg tagccggcct tggcgaggag
     1681 gcggaaagtg gcctcctgca ggctgccctt gggcaggccg atccgcagca cccgttcatc
     1741 caacccattc cagtccacca taatatctcc tttcgcacag gggtaaaaaa aaagcccgct
     1801 gaacaggtca gcgggcaaag tgaaaacacc attccaaacc ggttcgctat gagagggggg
     1861 cctccgcatg atggtgggaa tggtggtgat gtttttgaat ggtattgttc ataatctaat
     1921 gtagcgaaat cgccgtttaa atgcaacagg aaaaacggtc agcccagaac ccggagcgcg
     1981 gcctccgtat cttccagatc agtgaagaga tgatcgggat tacaggcggc gaggtggctc
     2041 cttgagtgag cggcggcggc cacagccacg gcgaccgccc cgtgcggccg ggcgcactgg
     2101 atgtcgtagg gggtgtcccc gatgacatag acctcctccg gccggggcga aaggccgctc
     2161 agatcgcgga aacgcgccac ggcgaagggg aggagctggt cgcgcacccc ggaatcatcg
     2221 gcaaaggcgc caaaagggaa atagcggtcc agtccgaaat cggccagttt gagatagccg
     2281 ctggtgcgcc agttgccggt gagcaggccg agatggacat ccgggcgccc gcttaaggcc
     2341 tcgagcagag ccggaatgcc gggcatgagc cgcttgcctg ctcccggcag gggcatctcc
     2401 tctgccagca gctcgaaata gcgctgtttg tactcctcct cgatctccgg agaatggggc
     2461 agggcgaaga tggccagggc atcctggagg atcgagctgt cggtgcgtcc ggagaggctg
     2521 accccggcca gggcgtcggg gatggcgtag agatcggtga aggcgcgggt catggcgcgc
     2581 aatccggctc cgccggagtg gacgagggtg ccatcgatat cgaagagcag cagcttcact
     2641 agaactcccc gctcacaccc agccccggtc cgtcgggaag gatcagccag ccgtccctga
     2701 ccgtcacccc ggtgaagggg tccctggcga ggaggaggtt gccatcgaga tcgggataat
     2761 cggtcagggg cgaaaggtgg gcggccgcag tgatcgagag cgagctcgaa accatgcagc
     2821 cgagcatgat cttgagcccg agggagcggg ccatccagat catgcgcagg gattcctgga
     2881 ggccgccggc cttgtccacc ttgatgttga tgccatcaaa ggcctcggcc agcctgggga
     2941 tatcggcgct ggtcttgacc gattcgtcgg cgaggatggg gatggaggcg cgttccttga
     3001 cccaggcggt ctcctcgagc atatggctcg gcatgggctg ctcgacgatg tcgacgccgt
     3061 tggccgccag ccactccacc ttgcgcaggg cgagttcttt ctctttccac ccctcgttgg
     3121 catcgacgcg cagggtcttt ttggtgacgc tgcgcaccgc cgcgaggatc tcctcatcgt
     3181 tgtccttgcc catcttgatc ttgaggaggg gatagggagc ggcctcctcc accttttgct
     3241 tgaccatctc catggtatcg atgccgatcg aaaatgtggt ctgaggagcc ttttgggggt
     3301 cgaggcccca gaactgatag agcgggatgc cgagggattt gctgatccag tccatgagag
     3361 cgatatcgat ggccgccttg gccgctgtct gtccctcgca gaccgcctgg acagcggcgt
     3421 tgacgtcgac gaacttccag ggatcgcagt tctcgaggat cggccttgcc ttttcgaggg
     3481 cggcgatgat tgactcgagg gattcgccgt agcggacgtt gtgggcagcc tcgccaagac
     3541 cgtaaattcc gtttttttca agctttacga aggcatattc cttgtaggtg ctggtgttgc
     3601 gggcgatggt ccaggcgtgg cggagatcaa ggcgcttgct cttgatctcc atcctgaagc
     3661 cgccgctgct cgacctgctg ctcttctggc acccggccag caggggtgaa gccgccgatg
     3721 cggctccggc cgcgatgatc cccttgccgg ttttatggat gaactctttg cgtgacatgg
     3781 acattctctt cctcctccga gtggttattc gcttctaaaa atacgccttt cgccgcaaaa
     3841 aggcaaacga aatgcgcatg cctgccggag ccgaccggcc gcaaagaacg ccggcgggcg
     3901 gctcacctcc agataagatg ttactttttc gcaaaaaatg cgtaaaatcg ggtgtacacc
     3961 gagccgggag agtgccatgt caggacaagg gtcaactgtg gatccgcagc tgcgcagggt
     4021 gctgcgtgac gatctgcgcc gcgggggcta tgccgcccag atccggcgtg aaaacgccga
     4081 gatgaaacag ttttatctaa gcgacgaaca gcgcctgcgg atccgttcca tgggtacggt
     4141 gcgccgcttt ttcgccgtga ctttctggct gctcaggagc ctgctgctgc atatgagcag
     4201 cttccgcagg ctcatgctca tcctcgccct gctcgtcatc tactccccgc gctgcgaatg
     4261 gcacggcacc tcattccagg ccagcagcga catcagcctt tccggggtgc tgctcctgct
     4321 cttcatcatc atgctcgagg tcaaggacaa gatgctggcg cgcagcgaac tgcaggcggg
     4381 gcgcatcgtc cagaaggccc tcatcccgaa ccagccgccg gccatcccgg gctggtcgat
     4441 ctggctctat taccacccgg ccaatgaggt agggggcgac ctgctcgact atctgccgat
     4501 caacagccat cgccacggct tcgccctcgg cgacatctcg gacaaggggc tgggggcggc
     4561 cctgctcatg gccaagctcc aggccaccct gcgcgccctg gcgccggaat gctccgatcc
     4621 agccgatctc ggccagaaga tgaaccgcat ctacattcgc gacaccatgg ccaaccgctt
     4681 cgcctcgatg atctatttcg aaatcgcttc gaatgagtcg aatatccgct tcatcaacgc
     4741 cggccatcag ccccccctgc tcgtccaggg cgatcaggtc agcgaatggc ccaagggcgg
     4801 ccccgccctg gggattcagc ccaatgccag tta
//
LOCUS       DAOPWU010000270         4821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_17685_length_4821_cov_5.096517, whole genome shotgun sequence.
ACCESSION   DAOPWU010000270 DAOPWU010000000
VERSION     DAOPWU010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17685_length_4821_cov_5.096517"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2221)
                     /locus_tag="PKM23_11950"
     CDS             complement(<1..2221)
                     /locus_tag="PKM23_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HNY92227.1"
                     /translation="MLNGKYQQLYNRLLKHIPSSRMFHDDLRTLALGTDASFYRLLPK
                     LAIRANDEIEICAILRECTALQLPLTFRAAGTSLSGQSISDSVLVVIDQGWKKITIEG
                     EGGELVRLQPGVLGCAANRALLPLGKKIGPDPASIDAAMIGGIAANNASGMCCGTAQN
                     SYRTLAGLRLSLADGTLFDGLDPEAVARFRRDGADLLNQLLELRRKLLGNAALTARVR
                     EKFRMKNTCGYSLNALADYEDPVDILQHLMIGSEGTLGFLAEIGYHTVPEYRDKGSSL
                     ILFPDIETACRAVAILKSAPVAAVEIMDRAALRSVEDKKGMPDYLAGLPERAASLLVE
                     TRAATPDLLQSQIASIHAALAALPVLRPLSFTLDPAEYSKLWNIRKGLFPAVGAMRPK
                     GTTVIIEDVAFPVPRLAEATLDLQGLFRKHGYDDTIIFGHALEGNLHFVFNQNFNIPA
                     EVKRYQNFMDEVCHMVVEKYDGALKAEHGTGRNMAPYVGLEWGEEAVEIMREIKRIFD
                     PAGILNPGVILNADPEIHLKNLKPLPEAHELIDKCIECGFCEPHCVANQLTLSARQRI
                     IVYREISRLEAAGEEPHRLAQLRDAYDYFGDQTCATDGLCALACPVDIDTGKLIKELR
                     REKASPRAKRVAGALARNMTGVTAGARIGLCIVHIFHLLLGPALLGGVARGLRRLSGN
                     RIPLWTPQMPGPGRRIATAGPSVQAALRRGEAPKVVYFPSCITRSMGPARGDSAGEDL
                     "
     gene            2528..3145
                     /locus_tag="PKM23_11955"
     CDS             2528..3145
                     /locus_tag="PKM23_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNY92228.1"
                     /translation="MELVKAIEQRRTVRRFSSAPVAAESLKELARRAGLAPSINNSQP
                     WKFIAVTRAEVIREMAAAVHQRVSQLFAGAEKENILRTVDHFSTVFEHAPAVLFVAAR
                     PYQAIADEIGSGETGHDALNAMRRHPDLQSVGAAVQNILLSATELGLGACWLSGLMVA
                     RPELEKVLGLEQPWELVTAIAIGTPESTPVPKQHPPVEQIFTLID"
     gene            3290..4210
                     /locus_tag="PKM23_11960"
     CDS             3290..4210
                     /locus_tag="PKM23_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNY92229.1"
                     /translation="MADFQNITGLIFNIMRFSVHDGPGIRTTVFVKGCPLQCAWCHNP
                     ESISPQPEMMLRTDICLMCGACIAACPVGAIENTAAGLVTHREKCIACGICAQVCPVE
                     ARVRVGREMTVSEVMLEILADRVFYDQSGGGVTFSGGEPTRQPDFLHALLTAARHEGL
                     HTIVDTCGYAPWSVFERIAPLTSRFLFDIKIVDPEKHRRWTGVSNRRILDNLTRLDAR
                     GCAISLRLPIIPGVNDSPEDIKGLVELLQRLHHTKRLHLLPFHGGGAEKYHRLGRAFH
                     LEQAVPPDHAAIERIAQDLRNHIEEVHIGG"
     gene            4258..>4821
                     /locus_tag="PKM23_11965"
     CDS             4258..>4821
                     /locus_tag="PKM23_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNY92230.1"
                     /translation="MMTERVRLLREKSLQAVPTLSTERAEIVTRIYRQAGLLSAPMLR
                     ARVFAAIMQEKEICINDGELIVGERGPAPKATSTFPELCCHSLQDLEILDSREKVFFR
                     VDEGTRRLYREEFIPFWRGRSMREMILASMSEEWKQAYEAGIFTEFMEQRGPGHTVLD
                     DKIYNKGMNDFIADIETRLAELDFVNDA"
BASE COUNT          949 a         1464 c         1514 g          894 t
ORIGIN      
        1 ggaggtcctc tccggcgctg tccccccgtg cgggacccat ggaacgggtg atgcaggagg
       61 gaaagtagac caccttcggc gcctcccccc tgcgcagggc ggcctgaacc gaggggccgg
      121 cggtggcgat tcgtcgccca ggtccgggca tctggggcgt ccagaggggg atacggttgc
      181 cggagagccg gcgcagtccg cgcgcgactc cgcccaacag tgcagggccg agaagcagat
      241 ggaaaatgtg gacgatgcac agtccgatgc gggcgcccgc agtgacgccc gtcatgttcc
      301 gggccagggc accggccacc cgtttggccc gcggggaggc cttttcccgg cgcagttcct
      361 tgatcagctt gccggtgtcg atgtcgacgg gacaggcgag ggcgcagaga ccgtcggtgg
      421 cacaggtctg atccccgaaa tagtcgtagg cgtctctgag ctgtgccagg cggtgcggct
      481 cctcgcccgc cgcctccagc cgggagattt cgcgataaac gatgatgcgc tgcctcgccg
      541 acagggtgag ctggttggcc acgcagtggg gctcgcaaaa gccgcattca atgcacttgt
      601 caatgagttc gtgcgcctcg ggcaggggtt tgaggttctt gagatggatc tccggatcgg
      661 cgttgaggat gaccccggga ttgagaattc ccgccggatc gaaaatccgc ttgatttcgc
      721 gcatgatctc caccgcctct tcgccccact cgagtcccac atagggggcc atgttgcggc
      781 cggtgccgtg ttcggccttg agcgccccgt cgtatttctc caccaccatg tggcagacct
      841 catccataaa gttctggtag cgtttcacct cggcggggat attgaaattc tggttgaaga
      901 cgaaatggag gttgccctcc agggcgtggc cgaagatgat ggtatcgtca tagccgtgtt
      961 tcctgaacag tccctgaaga tcgagggtgg cctcggccag acgcggcacc ggaaaggcga
     1021 catcctcgat gatgaccgtg gtgcctttgg ggcgcatggc gccgacggcg gggaagagcc
     1081 ccttgcggat attccagagt ttgctgtact ccgccggatc gagggtaaag ctgaggggcc
     1141 tgagcacggg gagggcggcc agggcggcat gaatcgaggc gatctggctc tgcagcagat
     1201 cgggcgtggc ggcgcgggtc tcaaccagca gggaggcggc cctctccgga agcccggcca
     1261 gatagtcggg catccccttt ttatcctcga ccgaacgcaa ggcggcgcgg tccatgatct
     1321 ccacagccgc gaccggtgcg ctcttgagga tcgcgaccgc ccggcaggcg gtttcgatgt
     1381 ccgggaagag gatcaggctg gagcccttgt cccggtattc cggcacggta tgatagccga
     1441 tctcagcgag gaagcccagg gtcccttccg acccgatcat gaggtgctgg aggatatcga
     1501 ccgggtcctc gtaatcggcg agggcattga ggctgtagcc gcaggtgttt ttcatcctga
     1561 atttttcccg cacccgggcg gtcaaagccg catttcccaa aagcttccgc cgcagctcga
     1621 ggagctggtt gagcagatca gcgccgtcgc ggcgaaaacg ggcgaccgct tcaggatcga
     1681 ggccgtcgaa gagggtgcca tcagcgaggc tgaggcgcaa gccggcgagt gtgcgataac
     1741 tgttctgggc ggtgccgcag cacatgccgc tggcgttgtt ggcggcgatg ccgccgatca
     1801 tggcggcgtc gatcgaggcg ggatcgggac cgattttttt gccgagaggc aggagagctc
     1861 tgttggcagc acagccgagc accccgggct gcaagcgcac aagctcaccc ccctccccct
     1921 cgatggtgat ctttttccac ccctgatcaa tgaccaccag gacggaatcg gagatggact
     1981 gcccggagag gctggtgccg gcggcgcgga aggtgagcgg cagctgcaag gcagtgcatt
     2041 cgcgcaggat ggcgcagatc tcaatttcat cattggcgcg gatagccagc ttgggcagta
     2101 gccggtaaaa gctggcgtcg gtgccgaggg ccagggtgcg cagatcgtcg tgaaacatgc
     2161 ggctggaggg aatgtgcttt aaaagccggt tataaagctg ttgatacttg ccgttcagca
     2221 tgaaagacct cgctggcgtt agtaggagcg cagcaggaaa gagagcagga ttaagaaccc
     2281 aaggttttgc cctcgcaggc catccgaatc accaaaagcg aggataatca ccaggatgag
     2341 ggcgcccttg accgtacccg taatatagga gcggtttatg caagattcaa gagaaaaatc
     2401 agcccatgct caccccgtta gcaatcgagg ggaaagcagc acgccggaag ctgcgccccg
     2461 cctcccggcg cgggaattaa tcgccgcccg gtggcattat ataggtgagt caatctcagg
     2521 aggaaacatg gaacttgtca aggccattga gcaaagaaga actgtacgcc gattctccag
     2581 cgcgcctgtt gcggcggaga gtctcaagga gttggcccgc cgggccggac tcgctcccag
     2641 catcaacaac agtcagccgt ggaaattcat cgccgtcacc cgcgcggagg tcatccggga
     2701 gatggccgcc gccgtccatc agcgcgtgag ccagctcttc gccggggcgg agaaggagaa
     2761 cattcttcgc acggttgacc atttttcgac cgtcttcgag cacgcgcccg ccgtcctctt
     2821 cgtcgcagcg cggccctacc aggctatcgc cgatgaaatc ggcagcggcg aaaccgggca
     2881 tgacgccctc aacgcgatgc gccgtcatcc tgatctgcag agcgtcggtg cggcagtgca
     2941 gaacatcctg ctctctgcca ctgagttggg gctcggtgcg tgctggctct ccggcctgat
     3001 ggtggcgcgg cccgagctgg agaaagtgct cgggctggag cagccctggg agctggttac
     3061 cgcaatagcc ataggcactc ccgaaagcac gcccgttccc aaacagcatc cccccgtcga
     3121 acagatcttc accctcatcg actgagcggc tttgctggcc ccggccggaa gggagaaaga
     3181 gccccttccg gccgcgcggc gttgaatact ggagggcttt ggaaatatct cttgcgcgat
     3241 tcgcaaggcg gagctatatt atagccgcga acccagcgaa atggagagga tggccgattt
     3301 ccagaatatt acgggcttga tctttaacat catgcgcttt tcggtgcatg acgggccggg
     3361 catccgcacc acagtttttg tcaagggatg ccccctgcag tgcgcctggt gtcacaatcc
     3421 cgaatcaatt tcaccgcagc cggagatgat gctgcgcacg gacatttgtc tgatgtgcgg
     3481 cgcctgcatt gccgcctgtc cggtgggggc gattgaaaac accgcagccg ggctggtcac
     3541 tcaccgggaa aaatgcatcg cctgcggaat ttgcgcccag gtctgccccg tcgaggcccg
     3601 cgtgcgcgtc ggccgtgaga tgactgtatc agaggtgatg ctggagattc tcgccgaccg
     3661 cgtcttttac gaccagtccg gagggggcgt cactttctcc ggcggcgagc caacccgcca
     3721 gcccgatttc ctgcacgccc ttctcaccgc cgcccggcat gagggactgc ataccatcgt
     3781 cgacacctgc ggttatgccc cctggagcgt ctttgaacgc atcgcccccc tgaccagccg
     3841 attccttttt gacatcaaga tcgtcgatcc ggaaaaacac cgtcgctgga ccggcgtctc
     3901 caatcgccgc atccttgaca atctgacccg acttgacgcc aggggctgtg ccatcagcct
     3961 gcgcctgccc atcatcccgg gtgtcaacga cagcccggag gatatcaagg ggctggtcga
     4021 gctactccag cgattgcacc ataccaagag gctgcatctc ctccccttcc acggaggtgg
     4081 agcggaaaaa tatcaccgtc tcggacgggc tttccatctc gagcaggccg ttccgcccga
     4141 tcatgcagcg atcgaaagaa ttgcgcaaga cctgcgcaac catatcgaag aggtccacat
     4201 cgggggctaa catgaacccc gaagccgtat cgccgcctac taatacaggg agctgccatg
     4261 atgaccgaac gcgtgcgcct tttacgcgag aaaagcctgc aagccgttcc caccctttcc
     4321 accgaacgcg ccgaaattgt cacacgcatc taccgtcaag ccggcctcct ctccgctccg
     4381 atgctgcgcg cccgcgtttt tgccgccatc atgcaggaaa aggaaatctg tatcaacgac
     4441 ggcgagctca tcgtcggcga acgcggcccc gcgcccaaag ccacctccac ctttccggaa
     4501 ctctgctgcc actccctcca ggacctggag atcctcgaca gccgcgaaaa ggtctttttc
     4561 agggtcgatg aggggacccg ccgcctctat cgggaggagt tcattccctt ctggaggggg
     4621 cgaagcatgc gggagatgat cctcgccagt atgagtgagg agtggaagca ggcttatgag
     4681 gcgggcatct tcaccgagtt catggagcag cgcggacccg gccacaccgt gctcgacgac
     4741 aagatctaca acaaggggat gaacgatttt atcgctgata tcgagacccg gctggctgag
     4801 ctcgacttcg tcaatgatgc c
//
LOCUS       DAOPWU010000271         4818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_17699_length_4818_cov_4.606760, whole genome shotgun sequence.
ACCESSION   DAOPWU010000271 DAOPWU010000000
VERSION     DAOPWU010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17699_length_4818_cov_4.606760"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            463..3420
                     /locus_tag="PKM23_11970"
     CDS             463..3420
                     /locus_tag="PKM23_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92231.1"
                     /translation="MAKIYQILAILLLAWSALLAQADLFDIGTDQNPPGISWRSLESP
                     HFQIIFPQEIAADAQRIASLLERVYPADCKTLGIQPGPLPLIMHSRTTESNGFVTLAP
                     RRSEWFSTPPQVGLTGPVGWYPLLAIHEYRHVVQFQALKQGFNRLLYWSLGEYGWLAG
                     AGISVPAWFFEGDAVVMETALTRSGRGRQPEFLMPIRTMLLSGERYSYYKMNLGSYKN
                     WIANPYEYGFYLNTWLRRQHGAELWPAVLKSTAKLSFIPYRFSWSLKRRTGMGLMKSH
                     AAMLAGLEAYYRTSRPQPGTAPAGNAAVRSNPGGNEMVGGGNAAVRRNQGGNEMVGGG
                     NEAVRRWDRDNDGFWTIYQAPRPAADGSLIVLKIGLADQTAFIRLHPDGREETLLHPN
                     PVDGSPHSIAGETLVWSEKIYDPRWSRQDFRTIKSLDLASGTRRTLVARGRHFAPALS
                     PDGANIAAILFEADNRCHLVLLSAQNGSEMRRFENPANDFLMTPTWSPDGREIVCSKL
                     CGSGRALTLISLDDGRMTDLIPPGDWEVTTLFHGGRHILFSSNDGEVMQIFAVERQSG
                     RLFRVTDRPFGAFYPALSGDGCRLFFGDYDLDGYHVAESTLDSSRWIPLDSLPVMENP
                     WFEPVTAQEAGPALLDNLAPQSWPVRPFTAWNDRPRIHSWQVYADDPSRTLGGSLFAQ
                     NLLSTLQASAGFDFDRDENAGAISADLTWARLYPLLSAGVTSGHRASTYLDENDKTLR
                     YTWKEKSARATILLPLNLSRNGYTTALSLQGAAQLTWISGISDDNAFERWENQSGWFR
                     TMGYGLEFYRYRKSGADIFPVLGQTLRMGYDHTPWQSHYRGERFLLRANLYLPGLVRR
                     HGLALRGDYERQRPESYRFSGDIRFPRGYSYRYQDRIGRLAVDYTLPLAYPDQHLWAL
                     LYVQRVKSLFFHDYGWSSLAGRRTFYRSSGVELSADVNFFSLPVILDVGVRLAWRWRE
                     KNWRTEATLNLPL"
     gene            3683..>4818
                     /locus_tag="PKM23_11975"
     CDS             3683..>4818
                     /locus_tag="PKM23_11975"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNY92232.1"
                     /translation="MKKWFALFSLLMLTLLLTPIAQAEWSNGKPADLVLGQTDFVSRV
                     SGTDASHFNGPNGVAIDPVSRKLFVVDRTNHRILRFGVADGLTSGAAAEAVLGQSDFA
                     GKSSGADAAKFNNPIGICFDAEGRLWVGDFSNNRVLRFDAAADKPSGAPADGVLGQPD
                     FVSKAAATTRAGMSGPVAPFIDHNGTLWVAQFNNHRVTRYDHAAAKANGADADGVLGQ
                     PDFTTSSSGLSASKMNNVNSLYVDSSGTLWVSEFTNRRVLRFDNAAAKTDGAEADGVL
                     GQPDFITNTATTTQSGMNSVRFVTGDAQGTLYVVEEPNQRVLVYSNAQTKANGGEADY
                     VLGQSDFVSKLNPIPPTEASFNIPRAAFYDDVAKTLWVTDFSNNR"
BASE COUNT          944 a         1655 c         1327 g          892 t
ORIGIN      
        1 tactggcggg gtaacaaatg acgtacgcac cgattccaac ggcgaggccc acttcgacca
       61 cgatcccaga agtggcaaga tcttcgtgga cgggaaaaaa gtctttgagg ggtggattga
      121 aggccgtgtc gttgtctatg tataatagca agattttacc gtccggaaag ctagtgatat
      181 ttctctggtg acaggttatc aacgatgccg cggttgaaga ctatattggg cgacaggcaa
      241 gcttacagcg cccccgcgat actgaatcga accattggat aataccttgg gaaatcgccc
      301 gagatctgga ttttaagcag ttgcgctggc ccgttggatg cccgaaaatc tcaaggagca
      361 agatggtcct gtaaacccgc cgcccagaag cagatcataa aattcctttt ctatcggcca
      421 tgaattgtgt atctttcagc agcactcatc cggaggcaat tcatggccaa aatctatcaa
      481 atcctcgcca tccttctgct ggcctggtcc gccctcctcg cccaggccga tctctttgat
      541 atcggcaccg accagaatcc gcccggcatc tcctggcgct cccttgagag cccccacttt
      601 cagatcatct tcccgcagga aatcgccgcc gatgcccagc gcatcgccag cctccttgaa
      661 cgcgtctatc cggcggactg caaaaccctc ggcattcagc ccgggcccct cccgctgatc
      721 atgcacagcc gcaccaccga gagcaacggc tttgtcaccc tcgccccgcg ccgcagcgag
      781 tggttcagca ctccacccca ggtcggcctt accggcccgg tcggttggta tcccctgctg
      841 gccatccacg aataccgcca cgtcgtccag tttcaggcgc tgaaacaggg ctttaaccgt
      901 ctgctctact ggtcgctcgg cgagtacggc tggctcgccg gggccggcat ctcggtcccg
      961 gcctggttct tcgagggcga cgccgtggtc atggagaccg cccttacccg cagcggccgc
     1021 ggccgccaac ccgaattcct catgcccatc cgcaccatgc tcctctccgg cgaacgctac
     1081 tcctattaca agatgaacct gggctcctac aaaaactgga tcgccaatcc ctacgaatac
     1141 ggtttttatc tcaacacctg gctgcgccgg cagcacggcg cggaactctg gccagcggtg
     1201 ctcaaaagca cggccaaact ctctttcatc ccctaccgtt tctcctggtc cctgaaacgc
     1261 cgcaccggca tggggctgat gaaaagccac gcggccatgc tggccgggct ggaggcttat
     1321 taccggacca gccggccgca gccgggaaca gcaccggcag ggaacgcggc agtaaggagt
     1381 aatccgggtg ggaacgagat ggtggggggc gggaacgcgg cagtgcggag gaaccagggt
     1441 gggaacgaga tggtgggggg cgggaacgag gcagtgcggc gctgggaccg ggacaatgac
     1501 ggcttctgga ccatttacca ggccccgcgc ccggccgcgg acggatcgct gatcgtcctc
     1561 aagatcggcc tcgccgacca gacggccttc atccgcctcc atcccgacgg ccgggaagag
     1621 acgctcctcc accccaatcc ggtcgacggc agcccgcact ccatcgccgg agagaccctg
     1681 gtctggtccg aaaagatcta tgacccgcgc tggagccgcc aggatttccg caccatcaaa
     1741 agcctggatc tcgccagcgg cacccggcgg accctggtcg cgaggggccg ccacttcgcc
     1801 cccgccctct cgccggacgg cgcaaacatc gccgccatcc tctttgaagc ggacaaccgc
     1861 tgccatctgg tcctgctctc tgcgcaaaac ggcagcgaaa tgcgccgttt cgagaatccg
     1921 gccaacgatt tcctcatgac cccaacctgg tcgcccgacg gcagggagat cgtctgcagc
     1981 aaactgtgcg gaagcggccg ggccctgact ctcatctccc tggatgacgg ccgcatgact
     2041 gacctcatcc cccccggcga ttgggaggtc accacccttt ttcatggcgg ccgccacatc
     2101 ctcttcagca gcaatgacgg ggaggtcatg cagatcttcg ccgtggagcg gcaaagcggc
     2161 cggctcttcc gcgtcaccga ccgccccttc ggggcctttt atcccgccct ctccggtgac
     2221 ggctgccggc tcttcttcgg cgattacgat ctcgatggct accatgtcgc ggaatcaacc
     2281 ctggattcat cccgctggat tcccctcgac agcctcccgg tgatggagaa cccctggttc
     2341 gagccggtca ccgcccagga ggccggcccc gccctcctcg ataaccttgc cccgcaaagc
     2401 tggccggtgc gccccttcac cgcctggaat gaccggccgc gcatccacag ctggcaggtc
     2461 tatgccgacg acccttcccg caccctgggc ggcagcctct tcgcccaaaa tctgctcagc
     2521 accctgcagg cctcggccgg attcgatttc gaccgggatg agaacgcggg cgccatcagc
     2581 gccgatctca cctgggcgcg gctctatccc ctgctcagcg cgggcgttac cagcggccat
     2641 cgcgcctcca cttatctcga cgagaatgac aaaacgcttc gctatacctg gaaggaaaag
     2701 agcgcccgcg ccaccatcct cctgccgctc aacctcagcc gcaacggcta tacgaccgct
     2761 ctttccctgc aaggcgccgc ccagctgacc tggatcagcg gaatcagcga cgacaacgcc
     2821 tttgagcgct gggaaaacca aagcggatgg ttcagaacca tgggctacgg cctcgagttc
     2881 taccgctatc gcaagtccgg cgccgacatc tttccagtcc tgggccaaac cctgcgcatg
     2941 ggctatgatc ataccccctg gcagagccat taccggggag agcgcttcct cctcagagcc
     3001 aacctctatc tgcccggcct ggtccggcgc catggtctgg cgctgcgcgg cgattatgaa
     3061 cggcagcggc cggagagcta ccgcttcagc ggggatatcc gctttccgcg cggctacagc
     3121 tatcgctatc aggaccgcat cgggcgcctc gccgtcgatt acaccctgcc cctcgcctat
     3181 cccgaccagc acctctgggc cctgctctac gtgcagcgtg tgaagagtct cttcttccat
     3241 gattacggct ggtccagcct tgcgggccgc cgcacttttt accggtcgag cggggtcgaa
     3301 ctgagcgccg atgtcaactt tttctccctg ccggtcatcc tcgatgtcgg ggtgcgtctg
     3361 gcctggcgct ggcgggagaa aaactggcgc actgaagcga ccctgaacct gccgttgtga
     3421 tttgctcccc gaaagagtgt acaacgcatc tgtcatgatt tcagcctgat tgacgagaaa
     3481 aggattgcat atattggggc cgttctttac attaacaaaa cctgcaaacg gttacatctc
     3541 cgttcaccgg tgaccgatta tcggctatcg ggcacccacc cttgaggttc cttatcttca
     3601 tgtaatagca aaaccaaaca catgtggcct ttgtgcagcc ggccgccccg acaccatcac
     3661 ctgatcccgg aaggagcagc tcatgaaaaa atggttcgcc ctcttcagcc ttctgatgtt
     3721 gacactcttg ttgacgccca ttgcacaagc cgaatggagc aacggcaagc ccgccgacct
     3781 cgtcctcggc caaaccgatt ttgtcagccg ggtgagcggc accgatgcca gccatttcaa
     3841 tggtcccaac ggcgtggcca tcgatccggt cagccgcaag ctcttcgtgg tcgaccgcac
     3901 caaccatcgc atcctccgtt tcggtgtggc agatggcctg accagcggcg cggctgccga
     3961 agcggtgctc ggccagagcg atttcgccgg caagagcagt ggagccgatg cggccaaatt
     4021 caacaatccc atcggcatct gcttcgacgc cgaagggcgg ctctgggtgg gggatttctc
     4081 caacaaccgc gtgctgcgtt tcgacgccgc cgcggacaag ccgagcgggg cgccggccga
     4141 cggcgtgctc ggtcagccgg actttgtctc caaagcggca gcaaccaccc gggccggcat
     4201 gtccggaccg gtcgccccct ttatcgatca caacggcacc ctctgggtcg cccaattcaa
     4261 caaccaccgc gtcacccgct acgatcacgc agcggccaag gccaacggtg cggatgcgga
     4321 cggcgtgctc ggccagcccg atttcaccac aagcagcagc ggcctttcgg cctcgaaaat
     4381 gaacaacgtc aacagcctct acgtcgatag cagcggcacc ctctgggtga gcgaattcac
     4441 caaccgccgc gtgctgcgtt tcgacaacgc cgccgccaaa acggacggcg ccgaggcgga
     4501 tggcgtgctc ggccagcccg atttcatcac caatacggcg acgaccactc aaagcggcat
     4561 gaacagcgtc cgctttgtca ccggcgacgc ccagggcacc ctctatgtcg tcgaggagcc
     4621 caaccagcgc gtgctggttt acagcaacgc ccagaccaaa gccaacggcg gcgaggccga
     4681 ctatgtgctc ggccagagtg atttcgtctc caagctcaat cccattccgc ccaccgaggc
     4741 ctccttcaac atccccaggg ctgcctttta cgatgacgtc gccaaaaccc tctgggtgac
     4801 ggatttttcc aacaaccg
//
LOCUS       DAOPWU010000272         4800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_17807_length_4800_cov_3.841517, whole genome shotgun sequence.
ACCESSION   DAOPWU010000272 DAOPWU010000000
VERSION     DAOPWU010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17807_length_4800_cov_3.841517"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1094)
                     /locus_tag="PKM23_11980"
     CDS             complement(162..1094)
                     /locus_tag="PKM23_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNY92233.1"
                     /translation="MKKFFSIGGVVLFAGALTLLSLAWINPERPRIVSCSSTMTEIVY
                     ELGGGEQMAGATSFCFFPEQVVEDKKSGRVAVVGDFIHIDYKAVEALKPDVILTDTNM
                     QRKHADSLRARGFKVLHFEPDHLEDVLSCIQEIGKAIGREENAAAMVQHMRQEFTTIR
                     QVSEKLPKTRVYMEINHMGPWTFGSASPLQDLIEIAGGTNVFGDTTAGVFMTNNAEVV
                     KRNPDIILSPIWLEAESGGWKGITPLYEIYSRPGYSETSAVKRSRVLYYDSALMKHFG
                     PRQVLATQKLAYLLHPENFPNPAETIPWELGWIK"
     gene            complement(1123..2040)
                     /locus_tag="PKM23_11985"
     CDS             complement(1123..2040)
                     /locus_tag="PKM23_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92234.1"
                     /translation="MENPVAAILLSALSGLSLALIGIAFRIGQSRNIFPLHIATSIGL
                     CGALFFGIQMDWGLLREIPLFIYILALLNAGGQILAMELTKVSLKRGPLSPVWCALNL
                     NFLVVIIYSAIAFHERITFYPMLALLFGILSVVAAANIGNTGSGDGNRKMTVRDKVVY
                     GFILFVILIANSVVFLTIKDLSTRLVPGKTITYLAAYLPNIYFILYLLMAVLCGAVVA
                     LQKNKVSRGFDLVKVGLLAGCGSIAGLFLLSLCARYQAALVFTVNGAVTILGGTLASV
                     FFFGEARSRAWYLTIGAAIAAVVLANFAR"
     gene            complement(2055..3041)
                     /gene="add"
                     /locus_tag="PKM23_11990"
     CDS             complement(2055..3041)
                     /gene="add"
                     /locus_tag="PKM23_11990"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNY92235.1"
                     /translation="MPKIELHLHLEGAFTLEFLLAQIQAIAPDQAPGSLAELQQRFIL
                     RDFDHFIETWIWKNRFFRSAQDFERSVYQTLASLSRQNVVHVEAFFSPWDFAASGAAA
                     PEIIRATIAGKLAAERDFGISCFLIADLNRDLGHETAGERLNDVMPHREHIVAIGLGG
                     SEARFPAAPFAGVFEEARQAGFHCVAHAGEAAGPDSIRAALDLLHAERIGHGITAIRD
                     PELMEELRRRQIPLEICPTSNIMTRVVEDYEHHPVRPFWQQGLLVTIHSDDPTMFNST
                     ITNEYQILHDRLGFELSDIRQLTLNAVHASFLCSEEKKRIIQSIGRFWQSVS"
     gene            complement(3122..4327)
                     /locus_tag="PKM23_11995"
     CDS             complement(3122..4327)
                     /locus_tag="PKM23_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNY92236.1"
                     /translation="MEYQVQDITLAPLGQTKIDWVAKWMKVVNQMAERFASDGVFNGI
                     DIAICIHLEAKTAYLAQTLHRLGARVWITSSNPLSTKDEVCAALAKSGVHVFARHGAS
                     PEEYHSYIRAITAAKPHVIVDDGGDICEYLHAHPEFAVNLRGICEETTTGVNRAKKLD
                     QAGALRYPVIGINDALSKYLFDNRYGTGQSTWTAITHLTNLSVTGKVVVIIGYGWVGR
                     GLAIRANGLGAEVIVTEINPWKAMEARMDGFRVMPIADAAGLGDYFITATGERSVLRF
                     EHMVKMKDGAMMANAGHFDFEIDVPELEEHIPAKKLVREEIEEYVLPNQHRIYLLAQG
                     GIINIAGGLGHPVEILDLSFGLQLTSVHYVLSTPGLKAQFYPVPQSIDETIIRARMEA
                     DGIAIDTPV"
     gene            complement(4393..>4800)
                     /locus_tag="PKM23_12000"
     CDS             complement(4393..>4800)
                     /locus_tag="PKM23_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92237.1"
                     /translation="ALAKDGKLHVSCTGDYGAPPGVVSVIDPATDTVVHTVNIGGAPG
                     NIALAPDAKLGFLSVWGAGALVYNTSTYAVVNGPANMWLGKGGSGILVDTDDYVWLSV
                     WDDDQVVKANAGGTILATYDVGDSPASLAQKID"
BASE COUNT         1057 a         1412 c         1352 g          979 t
ORIGIN      
        1 cctgataggc gcgctggatc gcaccctcgc ccagccggcg ctgcagttcc tcaagatctg
       61 actcaaacga actcatctca gcctctctct cgcctgacag gatactcggc aaagtcgatt
      121 gcactcaggc cttaccggtc aagaataatt atcgacgggg gctatttaat ccagcccaac
      181 tcccagggga tcgtctccgc gggattgggg aaattttccg gatgcagcag ataggccagc
      241 ttttgggttg ccagcacctg gcgcgggcca aaatgcttca tcagggccga atcgtagtat
      301 aaaacccggc tgcgcttgac ggcactggtt tcactgtatc cgggccggct atagatttca
      361 tacaggggag taatgccttt ccagcctccc gattccgcct ccagccagat cggtgagagg
      421 ataatgtcgg gattccgttt gacgacctcg gcgttattgg tcatgaacac gccggcggtg
      481 gtatcgccaa agacattggt gcccccggca atctcgatca ggtcctgcag cggagaagca
      541 ctgccgaagg tccacggccc catgtgattg atctccatat agacccgcgt tttaggcagt
      601 ttttcactca cctggcggat cgtcgtaaat tcctggcgca tatgctgcac catggcagcc
      661 gcattctctt cccggccgat ggctttgccg atctcctgta tgcaggagag cacgtcttcc
      721 aggtgatccg gctcaaaatg gagcaccttg aagccgcgcg cgcgcaggga atcggcatgc
      781 ttgcgctgca tgttggtgtc ggtgaggatg acatccggtt tcagcgcctc caccgcttta
      841 tagtcaatat ggataaaatc acccacgacc gctaccctgc cgcttttttt atcctcgacc
      901 acctgctccg ggaaaaaaca aaaactggtc gcaccggcca tctgttctcc cccgcccaac
      961 tcgtagacaa tctcggtcat ggtggatgaa cagctgacaa ttctgggccg ctccggattg
     1021 atccaggcga gagagagcag cgtcagcgca ccggcaaaga ggacgacgcc gccaatcgaa
     1081 aagaactttt tcataggcac cttcaatcaa agtgaattgt tattaccggg caaaattggc
     1141 gaggaccacc gccgcaatcg ccgcaccaat ggtcagatac caggcgcggc tgcgcgcctc
     1201 gccgaaaaag aagaccgagg ccagagtccc gccgagaatg gtgaccgccc cgttcaccgt
     1261 gaagaccagg gccgcctggt aacgggcgca aaggctgagc aggaacaggc ctgcaatcga
     1321 tccgcagccg gccagcagcc ccaccttcac caggtcaaat ccacggctga ccttgttctt
     1381 ctgcaaggcc accacagcgc cgcagagcac cgccatcaac agatatagga tgaaatagat
     1441 attgggcagg taggccgcca gataggttat tgtcttgccg ggcaccaggc gcgtactcaa
     1501 atccttgata gtcagaaaga cgacgctgtt ggcgatcagg atcacaaaga ggatgaaacc
     1561 gtaaaccacc ttgtcccgca cggtcatttt gcgattcccg tcaccactgc cggtattacc
     1621 gatattggcg gcggccacca cgctcaagat gccgaacagc agggccagca tcgggtagaa
     1681 ggtaatccgt tcatggaagg cgatggccga atagatgatc acgacgagga aattgaggtt
     1741 caaggcgcac caaaccggag agagcggccc tcttttcagg ctgaccttgg tcaactccat
     1801 ggccaggatc tggccgccgg cgttgagcag ggccagaatg taaatgaata acgggatctc
     1861 cctgagcaat ccccaatcca tctgaattcc gaaaaagagc gccccgcaga ggccgatgct
     1921 ggtggcgatg tggaggggaa aaatgttcct cgactgcccg atgcgaaagg cgatgccgat
     1981 cagggcaagg ctcaatcccg aaagggcgga taaaagaatg gccgcaactg gattttccat
     2041 tttatgcttc ctgattaaga gaccgactgc caaaaacgcc cgatcgactg gatgattcgc
     2101 ttcttttctt ctgaacacag gaacgaggca tggactgcat tgagggtgag ctgccggata
     2161 tcactcagct caaatccaag ccggtcatgc aggatctggt actcgttggt gatggtgctg
     2221 ttgaacatgg tcggatcatc ggaatggatg gtcaccagca gtccttgctg ccagaatggc
     2281 ctgaccggat ggtgctcata gtcctccacc acccgggtca tgatattgct ggtggggcat
     2341 atttccagag gaatctgccg cctgcgcagt tcctccatca gttccggatc ccggatggcc
     2401 gtaatgccat ggccgattct ttcggcgtga agcagatcga gggccgcgcg gatggaatca
     2461 gggccggcgg cctccccggc atgagccacg cagtgaaagc cggcttgcct ggcctcctca
     2521 aaaaccccgg caaagggcgc cgccggaaag cgagcctcgc ttccccccag cccgatggcg
     2581 acgatatgct cgcgatgcgg catcacatca ttcagccgct cgcccgcggt ctcatgcccg
     2641 aggtcccggt tcagatctgc gatcagaaaa cagctgatgc caaaatcccg ttcggccgcc
     2701 agcttgcccg caatggtggc cctgatgatc tccggcgccg cagcaccact ggcggcaaaa
     2761 tcccagggtg aaaaaaaagc ctccacatgg acgacatttt gccgcgacag gctggccagg
     2821 gtctggtaga ccgatcgttc gaaatcctgt gcggaacgga aaaaccggtt tttccagatc
     2881 caggtttcaa taaaatgatc aaaatcgcgc aggataaagc gctgctgcag ttcagccagc
     2941 gaacccggtg cctggtccgg agcaatggcc tggatctgcg ccagcaggaa ttccagggta
     3001 aaggctcctt ccaggtgcag gtgcagctcg atcttgggca tgcgcgaaag gatatcagcg
     3061 ttcttcatcg taccgggtca gacagttgat tggaaccggt gccagccacc gccggccttg
     3121 attacacagg tgtgtcaatg gcgatgccgt ccgcttccat ccgggcgcgg ataatggtct
     3181 cgtcgatgct ctgcggcacc ggataaaact gcgctttcag accgggagtg gagagcacat
     3241 aatgaaccga cgtcagctgc aagccaaagc tgagatcgag gatttccacc ggatggccca
     3301 gtccgccggc gatgttgatg atgccgccct gggcgagcag atagatacga tgctggttgg
     3361 gcagcacgta ttcctcgatc tcttcccgca ccagtttctt ggctggtata tgctcttcaa
     3421 gctccggcac atcgatctcg aaatcaaaat gaccggcatt ggccatcatg gcgccgtcct
     3481 tcattttgac catgtgttcg aagcgcagga cggaacgctc gccggtggcg gtgataaaat
     3541 aatctcccag tccggccgca tccgcgatcg gcatcacccg gaacccatcc atgcgtgctt
     3601 ccatggcttt ccagggattg atctcggtga cgattacttc agcgccgagt ccgttggcgc
     3661 ggatggccag tccccggccg acccagccgt aaccgatgat aaccaccacc ttgccggtga
     3721 cgctgagatt ggtcagatgg gtgatcgcag tccaggtcga ttgcccggtg ccatagcggt
     3781 tgtcgaagag atattttgac aaggcgtcgt taatgccgat caccgggtag cgcagggccc
     3841 cggcctggtc cagctttttg gccctgttca cgccggtcgt ggtctcttcg cagatgccgc
     3901 gcaggttaac ggcaaattcc ggatgggcat gcaggtattc gcaaatgtct cccccgtcat
     3961 cgacgatgac atgcggtttc gccgcggtga tcgcccggat gtaggagtga tactcctccg
     4021 gcgatgcgcc atgccgggca aagacatgaa ctccgctttt cgccagcgca gcgcagacct
     4081 catccttggt ggagagcgga ttgctgctgg tgatccacac cctggcccca aggcgatgca
     4141 gcgtttgcgc cagataggcg gttttggcct ccagatgaat acagatcgcg atatcgatgc
     4201 cgttaaaaac cccgtcggag gcaaaccttt cagccatctg gtttaccacc ttcatccatt
     4261 tggcgaccca gtcgattttg gtctgaccca ggggagccag ggtgatgtcc tgaacctgat
     4321 actccattgt gcaaactcct ctgcccgaat gaataaaaag gggatagact cctatcccct
     4381 tttgcgtgcc gtttaatcga ttttttgagc cagagaggcc ggactgtcac ccacatcgta
     4441 ggtggcgaga atcgtcccgc cggcgttggc cttcaccacc tggtcatcgt cccagacgct
     4501 gagccagaca tagtcatcgg tgtccaccag aattccggaa ccgcccttgc ccagccacat
     4561 attggccggc ccgttgacca ccgcataggt ggaggtattg tagaccagcg ctccggcgcc
     4621 ccagacggag aggaaaccaa gcttggcgtc cggcgccagc gcgatattgc caggtgcgcc
     4681 gccgatattc acggtgtgga cgaccgtatc ggtggcggga tcgatcacac tgaccactcc
     4741 ggggggtgcg ccatagtcac ccgtgcagga gacatgcagc ttgccatcct tggccagggc
//
LOCUS       DAOPWU010000273         4788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_17865_length_4788_cov_4.234101, whole genome shotgun sequence.
ACCESSION   DAOPWU010000273 DAOPWU010000000
VERSION     DAOPWU010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17865_length_4788_cov_4.234101"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1153)
                     /locus_tag="PKM23_12005"
     CDS             complement(14..1153)
                     /locus_tag="PKM23_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92238.1"
                     /translation="MPFPRLEPGRIRVFPLAGRMSKTVIRETAVDPEQAPPAAPEVAS
                     QAREAAARILSARARGASVILAFGAHLIKNGAAPLVIRMMERGWITHLATNGAGAIHD
                     WEFAYQGASEEDVRANVAQGRFGTWDETGRWLNLAVQVNILRGMGYGEAIGAMIAEDG
                     LLLPEVPILERELQENLRENHPLAPARAELLTTMRSFGLEGGRLAIDHPCKEYSIFGE
                     AFRLRLPVTVHCGIGYDIIYNHPLANGAALGRASHQDFQIFAQSVSGLEDGLFLSIGS
                     AIMAPQIFEKALSAVNNLRLQERQPILTRHLIVINDLQESHWDWSQREPPKNSPDYYL
                     RFLKSFHRMGGSVRYIAADNRLLLHHIYVALRASHPQPGAQGAHR"
     gene            1287..4130
                     /locus_tag="PKM23_12010"
     CDS             1287..4130
                     /locus_tag="PKM23_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNY92239.1"
                     /translation="MLPLRFRYIRFDEPLLNPAEWSGREALLLFPGEAGRHAAGRLLQ
                     GNWELTGLRLMTLEEFKEQLFVAPFQLLKEEKRTLAFFTSLDAPARDHFRISSYFQSI
                     ELANHFFSLWEEAGEAGLEMDAVAGDLAAAGAELLSWQEKSLRQLLHIRDLYHDTISR
                     RGFSDRIFIRREENLDYSLCSSFREIVLVNPFRLTRLEKSILHLLAERDHQITLISQM
                     PSKIMDEEEVEPRPFTAADLGPAAPGDITLHKCCNEFALYARFIEAAAQNRITHAVDV
                     SLQPSAFHHLLSPARFRLPASIPMSETSLYHFFATLAALLEELVWESRAGRLLLPLPA
                     LLEAAHSPGFYLPLLESADPEQRAAQRERLITLLHRLQEQDYLYLDLQGRFFNSAQRE
                     RRDLEPLARPVLRLIDRLLQVRDPAGLVRLIADPEGVPVLRIVPDKERACTTLIELFY
                     QSLADFSAIGESGIVEEWPALLPGEGPTTAARGILRLFLDYIKSARVRYEWNVDRQNQ
                     IEFTNFTEAQDLTFERLALLQVVEGQLPRTRAIPWLLTEQQRALLGLETADDTRLREK
                     YAFFRLALSTPELHLFTIENIEKNTQPSSFIEELALTFPENVRIISHPDIEYRDFGRQ
                     FFREGGEENLPLPVRGREDFFTLPFDGAADFPAGEWRLSFYAWEQVKSNPFEYALRNV
                     GRVPAWPAQWQTEWTHKLLGKVAQEVFDLCWHHFNQDALPFSRFDRIFSRYGEKAIAS
                     LFADQSDLYFKLPKNHGLAYFREFVLPLIRSSCDYFYQQLHERFHLDEDNPRVYPEKE
                     FTSARETSPRLLLAAAESQLPLDLLLTGRADLRIEYGEPTSAFLIDYKTGTDYKDEQL
                     WFYELFYYLIDDPAMAGRVRSCFFQILERRFDKLNKNTKKLSKTEFLTKLRGDIASTL
                     LEVCTLGYRPARRRSWGDKNVDDIIRMEIYHPR"
     gene            4192..>4788
                     /locus_tag="PKM23_12015"
     CDS             4192..>4788
                     /locus_tag="PKM23_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNY92240.1"
                     /translation="MGGFFGIVSQNDCVNDLFYGTDYHSHLGTRRGGMAVAQPEGFKK
                     SIHSIETAQFRSKFADDLAKFKGTMGIGVISDYEDQPLLIASHLGNYAIVTVGAVANL
                     AELAARELKSRHGHFTEMNLNAINPTELVASLINQEESFAAGMRKAQELIEGSCSILI
                     LTAAGIYAARDRLGRTPVLIGRRPDATAVAIESSAFPNL"
BASE COUNT          914 a         1534 c         1399 g          941 t
ORIGIN      
        1 agcagagggg agactatcga tgggcccctt gcgctcccgg ctgtggatga cttgctctca
       61 gtgcgacata gatatggtga agcaggagcc ggttgtcagc tgcgatgtag cgcacgcttc
      121 cgcccatgcg gtgaaaggat ttgaggaagc gcaaataata atccggagaa tttttaggcg
      181 gctctcgctg cgaccagtcc caatggctct cctggagatc gttgatcacg atgaggtgcc
      241 gggtgaggat gggctggcgc tcctgcaggc gcaggttgtt gaccgcggag agggcctttt
      301 cgaaaatctg cggggccatg atcgccgaac cgatggagag gaaaagacca tcctccagtc
      361 cggagacgga ttgggcaaag atctggaaat cctggtggct ggcacggccc agggcggcac
      421 cgttggcgag agggtgattg tagatgatgt cgtaaccgat gccgcagtgt acggtgaccg
      481 gcagccgcag gcggaaggcc tctccaaaga tcgagtactc cttgcaggga tggtcgatgg
      541 cgagccggcc gccctcaagg ccgaatgagc gcatggtggt gagcagctcg gcacgtgcgg
      601 gcgcgagggg atggttttca cgcaggttct cctgcagctc cctctcgagg atgggcacct
      661 caggcaaaag caggccgtct tcggcgatca tcgctccgat ggcctcacca tagcccatgc
      721 cgcgcagaat gttgacctgc accgccagat tgagccagcg tccggtctca tcccaggtgc
      781 cgaaccgccc ctgggcgaca ttggcgcgaa catcttcctc cgaagccccc tgataggcga
      841 attcccagtc gtggatggct ccggcaccgt tggtggcgag atgggtgatc cagcccctct
      901 ccatcatccg gatcaccagc ggggcggcgc cgtttttaat caggtgggct ccgaaggcca
      961 ggatcacact ggcgccgcgg gcgcgcgctg aaaggatgcg cgcggcggcc tcgcgggcct
     1021 ggctggcgac ctcgggagcg gcgggcggcg cctgctccgg gtctacggcg gtctcgcgga
     1081 tgacggtctt gctcatgcgc ccggcaaggg ggaagacgcg aatccgcccg ggttcaagtc
     1141 gggggaatgg catacggact cctggtttga acggcataaa gtaagggttt tcatcgatta
     1201 tgcaaagacg tttttttatt gcgtcgatac ttttatgtgc gtaaattgac accgtccctt
     1261 gcgcccatta agaggtctga cgccgcatgc ttcccctccg tttccgttat atccgcttcg
     1321 atgaacccct tctgaacccg gcagagtgga gcggccgtga ggccctgctg ctctttccgg
     1381 gtgaggccgg ccgccatgcc gccgggcgcc tgctgcaggg gaattgggag ctgaccggcc
     1441 tgcgcctgat gacgctggag gagttcaagg agcagctctt tgtcgccccc tttcagctgc
     1501 tcaaggagga gaagcggacc ctcgcttttt tcaccagcct cgacgccccc gcccgggacc
     1561 atttccgcat ctcctcctat ttccagagca tagaactcgc caaccacttc ttctcgctct
     1621 gggaggaggc cggcgaggcc gggctggaaa tggacgcggt cgccggagat ctcgccgccg
     1681 ccggggccga gctgctctcc tggcaggaga agagcctgag gcagctgctc cacatccgcg
     1741 acctctatca cgatacgatc agccggcggg gctttagcga ccgcatattt atcaggcgcg
     1801 aggagaatct cgactactcc ctctgctcct cattcaggga gatcgtcctg gtcaaccctt
     1861 tcaggctcac ccgtctggaa aagtcgattc tccatctgct tgcggagagg gatcaccaga
     1921 tcaccctcat cagccagatg ccgtcgaaaa tcatggatga ggaggaggtt gagccgcggc
     1981 ccttcactgc tgccgatttg gggccggccg cccccgggga catcaccctg cacaagtgct
     2041 gcaacgagtt cgccctctac gcccgcttca tcgaggccgc cgcacagaac cgcatcaccc
     2101 acgcggtgga tgtctcgctg cagccctccg cctttcacca tctgctctcg ccggccagat
     2161 tccgcctgcc ggcttcaatt cccatgagtg agacctccct ctaccacttt tttgccaccc
     2221 tcgcagccct gcttgaggaa ctggtctggg agagccgtgc cggccgcctg ctgctgccgc
     2281 tgcccgcact gctcgaggcg gcgcattcgc cgggattcta tctgcctttg ctcgagagcg
     2341 ctgatccgga acagcgggca gcccaaaggg agaggctcat caccctgctg cacaggctgc
     2401 aggagcagga ctatctctac ctcgacctgc agggccgttt tttcaattcc gcgcagcggg
     2461 agcggcgcga tctcgagccc ctggcccggc cggtgctgcg gctgatcgac aggctgctgc
     2521 aggtccggga tccggccggc ctggtccggc tcatcgccga tccggaaggg gtgcctgttc
     2581 tccgcatcgt cccggataaa gagcgggcct gcacaaccct catcgaactc ttttatcaaa
     2641 gcctcgccga tttcagcgcc attggtgaat cgggtattgt cgaggaatgg cctgccctgc
     2701 tcccgggcga agggccgact accgctgcac gcggcatcct tcgcctcttc ctcgactata
     2761 tcaagtccgc gcgcgtgcgc tacgaatgga acgtcgaccg ccagaaccag atcgagttca
     2821 ccaactttac cgaggcgcag gatctcacct tcgaacggct ggcgctcctg caggtggtgg
     2881 aggggcagct gccccgcaca cgcgccatcc cctggctgct caccgaacag cagcgcgccc
     2941 ttctgggcct cgagaccgcc gacgacaccc ggctgcgtga aaagtatgcc ttcttcaggc
     3001 tcgccctctc aacgcccgaa ctccacctct tcaccatcga aaatatcgag aaaaacaccc
     3061 agcccagttc cttcatcgag gagctggctt tgacctttcc cgaaaacgtg aggatcatca
     3121 gccatcccga catcgagtac cgggattttg gacggcagtt tttccgggaa gggggggagg
     3181 agaaccttcc gttgccggtc cgcggccggg aggacttttt taccctcccc ttcgatggcg
     3241 cggccgattt cccggccgga gagtggcgcc tctccttcta cgcctgggag caagtgaaat
     3301 ccaatccctt cgagtacgcc ctgcgcaacg tcggccgggt gccggcctgg ccggcgcaat
     3361 ggcaaaccga gtggacccac aagcttctcg gcaaggtggc ccaggaggtt ttcgatctct
     3421 gctggcatca cttcaaccag gatgccctgc ccttcagcag atttgaccgc atcttttccc
     3481 gctacggcga gaaggcgatc gcctccctct tcgccgatca atcggatctc tatttcaagc
     3541 tgcccaaaaa ccacggtctt gcctatttcc gcgaatttgt cctgccgctc atccgcagct
     3601 cgtgcgacta tttctatcag cagctgcatg agcgcttcca cctcgatgag gacaatccgc
     3661 gcgtctatcc ggaaaaggag ttcaccagcg ccagggagac cagccccaga ctcctccttg
     3721 ccgccgcgga gagccagctc ccgctggatc tcctgctgac cggccgcgcc gacctgcgca
     3781 tcgaatacgg cgaaccgaca tcggcctttc tcatcgacta caagaccggc acggactata
     3841 aggacgaaca gctctggttt tatgaactat tttattacct cattgacgat ccggccatgg
     3901 cagggcgggt gcgctcctgt ttcttccaga tcctcgagcg gaggttcgac aaactcaaca
     3961 agaacaccaa aaaactcagc aagaccgagt tcctgaccaa actccgcggc gatatcgcca
     4021 gcactttgct cgaggtctgc accctgggct atcggcccgc ccgcagacgc agctgggggg
     4081 acaagaatgt ggatgatatc atccgcatgg agatctatca tccccgctga acggtggggg
     4141 gagcaggctg acacaaactg aattccaagc agagaggagc cgaagcagca gatgggcggt
     4201 tttttcggga ttgtatcaca gaacgactgc gtcaacgatc ttttttacgg caccgactat
     4261 cacagccatc tcggcacccg gcgcggaggc atggcggtgg cccagccgga gggctttaag
     4321 aaatcgatcc acagcatcga gactgcgcag ttccgctcca aattcgccga tgacctggcc
     4381 aaattcaagg gcacgatggg catcggcgtc atcagcgatt acgaggacca gcccctgctg
     4441 atcgcctccc acctcggcaa ttacgccatt gtcactgtcg gcgcagtcgc caatctggcg
     4501 gaactcgccg cgcgcgagct caagagccgg cacggccatt ttaccgagat gaatctcaac
     4561 gctatcaatc caaccgaact ggtggcctcc ctgatcaacc aggaagagtc cttcgccgcg
     4621 ggtatgcgca aggcccagga actcatcgag ggctcctgtt ctatccttat tttaaccgcg
     4681 gcggggatct atgctgctcg cgaccgcctc gggcgcaccc cggtgcttat cggccgccgc
     4741 cccgacgcca cagccgtagc tattgaaagc agcgcttttc ccaatctc
//
LOCUS       DAOPWU010000274         4769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_17978_length_4769_cov_4.226347, whole genome shotgun sequence.
ACCESSION   DAOPWU010000274 DAOPWU010000000
VERSION     DAOPWU010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17978_length_4769_cov_4.226347"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKM23_12020"
     CDS             <1..347
                     /locus_tag="PKM23_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92241.1"
                     /translation="MKLSHLVEDKIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVW
                     ALEAYGAAYTLQEILTVKSDDVRGRSKAYEAIVKGESIPDPGLPESFNVLIRELMGLG
                     LDVELISRPAKK"
     gene            485..4765
                     /gene="rpoC"
                     /locus_tag="PKM23_12025"
     CDS             485..4765
                     /gene="rpoC"
                     /locus_tag="PKM23_12025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNY92242.1"
                     /translation="MFAQEHPTQDANFQAIYIGLASPDKILERSHGEVTKPETINYRS
                     FKPEKDGLFCEKIFGPVRDWECHCGKYKRIRYKGIVCDRCGVEVTMKSVRRERMGHIT
                     LAVPVVHIWYWKSLPSKIGYILGMSIKELEKVIYYESYVVVRPGKSGLSAKELITEDD
                     YLDLVERMRQEETDLPEEQRFYAEIGGTAIHALLRHVHIDALSHELRYMARNETSIQR
                     KAEALKRLKVVEAFRRSNRDRENRPDWMVMSVIPVVPPELRPLVPLEGGRFATSDLND
                     LYRRVINRNNRLKKLLEIKAPEVILRNEKRMLQEAVDSLFDNGRKSSAVRSDGNRALK
                     SLSDMLRGKQGRFRQNLLGKRIDYSGRSVIVVGPELHLHECGLPKEMALELFKPFVIR
                     KLVERGLVKTVKSAKKYVERRGSEVWDILEEIIDDHPVMLNRAPTLHRLGIQAFQPVL
                     IEGKAIQIHPLVCAAFNADFDGDQMAVHVPLSFYAQIETKILMLSSHNILSPAHGRPL
                     AIPSQDIVLGIYYLTKRRTGVQGEGMTFASTDEVLIAYNDQRIALHAPIKVRFNGEMI
                     ETTVGRVIFNDILPEDLKFVNVLLTKKAIEDLVARAYNKLGNHLTAQLLDNLKDIGFQ
                     FAMRSGTTVGLDDVLVPPEKEELLGQAFKNVEAILKRYERGIITDGERYNQIIDIWTH
                     TTSAVAEKMFERLRQDRQGFNPIFMMADSGARGSKEQIRQLAGMRGLMAKPQKKMTGS
                     MGEIIENPITANFREGLSVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVAQDVI
                     ITQDDCGTILGLRVADIKEGEEVIESMQDRIIGRVAAEDIFDPESGDVIVEAGALINE
                     EIAKKISDAGPESVMIRSVLTCEAPRGVCAQCYGRNLATGKMVCIGEAVGVMAAQSIG
                     EPGTQLTLRTFHIGGTASRIAAQSQVAVKHEGIIAWDNLKTVEREDGEVVCIGRNGRI
                     NVQDDDNRNMERLTVPYGATVIVAQGDRVAKGTVIFKWDPYTASILSTTSGKVEFRDL
                     LENVTYREEMDETTGMRQRVVIETRTRNLSPTINILDEKGKVTSAYVIPTRANLVVRD
                     GDMINAGNALVKIPREIAKTRDITGGLPRVAELFEARRPKEPAVVSEIDGIVEFGEMK
                     RGVRKIIVRSEDGHEEKVYMIGYGKHVLVHAGDFVHAGEKLSEGSVVPHDILAIMGAN
                     KVQEYLVNEIQEVYRLQGVRINDKHIEVIVRQMLQKVKIEDPGDTFYLEGDQVDRLGF
                     LAENERIRNMVIVTERGESKFQFEEMVEADEFRKAVAKLEAAEKTVPQARSGQPATFQ
                     PLLLGITKASLTTESFISAASFQETTRVLTDASVEGKVDELLGLKENVVMGHLIPAGT
                     GINLYKELKVEGPEIDLPAPPERPAPPPREEGEEDDDALLELDELENE"
BASE COUNT         1000 a         1601 c         1375 g          793 t
ORIGIN      
        1 tgatgaagct cagccatctg gttgaggaca agatccacgc ccgctccatc ggcccctatt
       61 cgctgatcac ccagcagccc ctgggcggca aggcgcagtt cggcggccag cgcttcggtg
      121 aaatggaagt ctgggcgctc gaagcctacg gcgcagccta taccctgcag gagatcctca
      181 ccgtcaagag cgatgacgtg cgcggccgtt ccaaggccta cgaagccatc gtcaagggcg
      241 agagcatccc cgatccgggc ctgccggaat ccttcaacgt cttgattcgt gaactgatgg
      301 gcctcggcct ggatgtggaa ctgatcagca gaccagcgaa gaaataatca gcccggatgg
      361 gttgtcccct caagatatca gaggtgcggc ggatgcaagg gagaccggtc atccgccccg
      421 cctctttatg tgttttcctt aaccaggctc gtgcacagag tttggaggtg accatttgaa
      481 ttacatgttc gctcaggaac atcccacaca ggatgccaat tttcaggcca tctacatcgg
      541 tctcgcttcg ccggacaaga tcctcgaacg ctcccatggc gaggtgacca aaccggagac
      601 catcaattat cgctccttca agcccgagaa ggacggcctc ttttgtgaaa agattttcgg
      661 cccggtgcgc gactgggaat gccactgcgg caaatacaag cgcatccgct acaagggcat
      721 cgtctgcgac cgctgcggcg tcgaggtgac gatgaagagc gtccgccgcg agcgcatggg
      781 ccacatcacc ctggccgtgc ccgtggtgca tatctggtac tggaaatccc tcccctccaa
      841 gatcggctac atcctcggca tgagcatcaa ggagctggag aaggtgatct attacgagtc
      901 ctatgtggtg gtgcgcccgg gcaagagcgg actctccgcc aaggaactga tcaccgagga
      961 tgactatctc gacctggtcg agcgcatgcg ccaggaggag accgacctgc ccgaggagca
     1021 gcgtttttac gctgaaatcg gcggcacggc catccatgcc ctcctccgcc atgtccatat
     1081 cgacgccctc tcccacgagc ttcgctatat ggcgcgcaac gaaacctcga tccagcgcaa
     1141 ggccgaagcc ctcaaacgcc tcaaggtggt ggaggccttc cgccgctcca accgcgaccg
     1201 cgaaaaccgc ccggactgga tggtgatgtc ggtcattccg gtggtgccgc ccgaactgcg
     1261 ccccctggtc cccctcgagg gcggacgctt cgccacctcg gacctcaacg atctctaccg
     1321 ccgcgtcatc aaccgcaaca accgcctcaa gaagctgctt gagatcaagg ctcccgaggt
     1381 gatcctgcgc aacgagaagc ggatgctcca ggaggcggtc gactccctct tcgacaacgg
     1441 ccgcaagtcc tcggccgtgc gcagcgacgg caaccgcgcc ctcaagagcc tctccgacat
     1501 gctgcgcggc aagcagggtc gtttccgcca gaacctgctc ggcaagcgca tcgactattc
     1561 cggccgctcg gtcatcgtag tcggccccga actccacctc catgaatgcg gcctgcccaa
     1621 ggagatggcc ctcgagctct tcaagccctt cgtcatccgc aagctggttg aacgcggcct
     1681 ggtcaagacc gtcaagagcg ccaagaaata tgtcgagcgc cgcggctccg aggtctggga
     1741 tatcctcgag gagatcatcg acgatcatcc cgtcatgctc aaccgcgccc cgaccctcca
     1801 tcgcctcggc atccaggcct tccagccagt gctcatcgag ggcaaggcga tccagattca
     1861 tcccctggtc tgcgccgcct tcaacgccga ctttgacggc gaccagatgg ccgtgcatgt
     1921 gccgctctcg ttctatgccc agatcgagac caagatcctc atgctctcga gccacaacat
     1981 cctctccccg gcccacggcc ggccgctggc aatccccagc caggatatcg tgctcggcat
     2041 ctattacctc accaagcgcc gcaccggcgt tcagggtgag gggatgacct tcgcttcgac
     2101 cgatgaagtg ctgatcgcct acaacgacca gcgcatcgcc ctgcacgccc cgatcaaggt
     2161 gcgcttcaac ggcgagatga tcgagactac cgtcggccgc gtcatcttca acgacatcct
     2221 ccccgaggat ttaaagttcg tcaatgtcct tctcaccaaa aaggcgatag aggacctggt
     2281 ggcgcgcgcc tacaacaaac tcggcaacca cctcaccgcc cagctccttg acaacctcaa
     2341 ggacatcggc ttccagttcg ccatgaggtc gggcaccacc gtcggcctcg atgacgtgct
     2401 ggtaccgccc gaaaaggaag agctgctcgg gcaggccttc aagaacgtcg aggccatcct
     2461 caagcgctat gagcgcggca tcatcactga cggcgagcgc tacaaccaga tcatcgacat
     2521 ctggacccac accaccagcg ccgtggccga aaagatgttc gagcgcctgc gccaggatcg
     2581 ccagggcttc aatcccatct tcatgatggc cgattcgggc gcgcgcggct ccaaggagca
     2641 gattcgccag ctcgccggca tgcgcggcct gatggccaag ccccagaaga aaatgaccgg
     2701 ctccatgggc gaaatcatcg agaatccgat cacggccaac ttccgcgaag gcctctcggt
     2761 gctcgagtac ttcatctcca cccacggcgc ccgcaagggt ctggccgata ccgccctcaa
     2821 gaccgctgac gccggctacc tcacccgccg tctggtcgat gtcgcccagg acgtcatcat
     2881 cacccaggat gactgcggca ccatcctcgg cctgcgcgtc gccgacatca aggaaggcga
     2941 agaggtgatc gagtcgatgc aggaccgaat catcggacgc gtcgccgccg aggatatttt
     3001 cgatcccgag tcaggcgacg tcatcgtcga ggccggcgcc ctcatcaacg aagagatcgc
     3061 caaaaagatc agcgacgccg gccccgagtc ggtgatgatc cgctcggtcc tcacctgcga
     3121 agccccgcgc ggggtttgcg cccagtgcta cggccgcaac ctggctacgg gcaagatggt
     3181 ttgcatcggc gaggccgtgg gtgtgatggc ggcccagtcg atcggcgaac cgggcaccca
     3241 gctcaccctc cgcacctttc acatcggtgg aaccgcttcg cgcatcgccg cccagtccca
     3301 ggtcgcggtc aagcatgagg gtatcatcgc ctgggataac ctcaagaccg tcgaacgcga
     3361 ggacggcgag gtggtctgca tcgggcgcaa cggccgcatc aatgtgcagg atgacgacaa
     3421 ccgcaacatg gagcggctca ccgtccccta cggcgcgacc gtcatcgtcg ctcagggcga
     3481 ccgcgtggcc aagggcacgg tcatcttcaa atgggatccc tacaccgcca gcatcctcag
     3541 caccacctcg ggcaaggtcg agttccgcga cctgcttgag aacgtcacct accgcgagga
     3601 gatggacgag actacgggca tgcgccagcg ggtggtcatc gagacccgca cccgcaacct
     3661 cagccccacc atcaacatcc tggatgaaaa gggcaaggtc accagcgcct acgtcatccc
     3721 gacccgggcc aacctggtgg tgcgcgacgg cgacatgatc aatgccggca acgccctggt
     3781 caagatcccg cgcgagatcg ccaagacccg cgacatcacc ggtggtctgc cccgcgtcgc
     3841 cgagctcttc gaggcccggc ggcccaagga gccggccgtg gtcagcgaaa tcgacggcat
     3901 tgttgaattc ggcgagatga agcgcggcgt gcgcaagatc atcgtccgct ccgaggacgg
     3961 ccatgaggaa aaggtctaca tgatcggcta cggcaagcac gtgctggtcc acgccggcga
     4021 tttcgtccac gcgggcgaaa agctctctga aggctcggtt gtcccccacg acatcctcgc
     4081 catcatgggc gccaacaagg tccaggaata cctggtcaac gaaattcagg aggtctaccg
     4141 cctgcagggc gtgcgcatca acgacaagca catcgaggtc atcgtgcgcc agatgctgca
     4201 gaaagtcaag atcgaggatc cgggcgacac cttttacctc gagggtgacc aggtcgaccg
     4261 tctcggcttc ctcgccgaga acgaacgcat ccgcaatatg gtgatcgtca ccgagcgcgg
     4321 cgaatccaag ttccagttcg aggagatggt cgaggccgac gagttccgca aggcggtggc
     4381 caaactcgaa gccgccgaga aaaccgtgcc gcaagcgcgt tcgggccagc cggccacctt
     4441 ccagcccctg ctgctcggca tcaccaaggc ctcgctgacc accgagagct tcatctcggc
     4501 ggcttcgttc caggagacca cgcgcgttct caccgacgcc tcggtcgagg gcaaggtcga
     4561 tgagctgctc ggcctcaagg agaatgtggt catgggccac ctcatcccgg ccgggaccgg
     4621 catcaacctc tacaaggagc tcaaggtcga gggaccggag atcgatctgc cggcgccacc
     4681 ggagcggccc gctcccccgc cgcgcgagga gggggaggag gatgacgacg ccctgctcga
     4741 gctcgatgaa ctggaaaatg aatagaaat
//
LOCUS       DAOPWU010000275         4760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_18028_length_4760_cov_3.827205, whole genome shotgun sequence.
ACCESSION   DAOPWU010000275 DAOPWU010000000
VERSION     DAOPWU010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18028_length_4760_cov_3.827205"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1590
                     /locus_tag="PKM23_12030"
     CDS             70..1590
                     /locus_tag="PKM23_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNY92243.1"
                     /translation="MKKIFLALLLFSSLTRAAIYVSPDGSDLNPGTLEKPLKTIPKAV
                     IKAAGGDTIFLRAGIHELTANVTIGDTKSGTEIKRSCLWGYPGERPLIDCSKEALGSR
                     AFTLKASWWHFRDFDLKGAGDNGLQITSVTGAYNLIENCTFFECRDSGIQLSAGAHHN
                     RIINCDSYHNADPPDYGDADGFAPKLDVGSDNYFYGCRAWGNCDDGWDGYMRGATNVT
                     TILENCWTWGNGWLKDGSDPGSQANGNGFKMGGGDNSNRDMLMHHFILTHCLAFNCKA
                     KGFDQNNNVGSMTLYNCTGYGNGTANYRITKPLAAGQTLTVKNCLSFNGGAELGSFAV
                     QEANSWLAPFVVTAADFVGVDYALAAAPRKADGSLPDIPFMHLALGSDLIDGGVGLGL
                     PFLGAAPDLGAFESDYRSMVTSLEAAPEGFRLEQNYPNPFNPATTIAYALPQPERVSL
                     RIYNLQGQEVASLVEGWHPAGEHRVVWRPQGLAAGIYIARLQAGIFNATRRLVLAK"
     gene            complement(1662..2369)
                     /locus_tag="PKM23_12035"
     CDS             complement(1662..2369)
                     /locus_tag="PKM23_12035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNY92244.1"
                     /translation="MDEQVVSMPRIGDKAPAFKAVTTQGEINFPEQYAGSWVILFSHP
                     ADFTPVCTSEFMTFATMEKQFNEANCKLVGLSVDGLYSHIAWLRTIKEKIEYKGMKNV
                     EVTFPLIEDITMEVAKKYGMIQPGEATTKAVRAVFFIDPKGIIRTIIYYPLSLGRNFD
                     ELYRVVIGLQTADAFSVALPADWRPGDDVIVPTAGSCGVAKERMENKKDMTCYDWFFC
                     TKKLAKELVLSTILKKK"
     gene            complement(2496..>4431)
                     /gene="katG"
                     /locus_tag="PKM23_12040"
     CDS             complement(2496..>4431)
                     /gene="katG"
                     /locus_tag="PKM23_12040"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNY92245.1"
                     /translation="RMAWHSAGTYRVSDGRGGASDGTQRFAPLNSWPDNANLDKARRL
                     LWPIKQKYGNKISWADLMILAGNCALESMGFKTFGFGGGREDIWQPEEETYWGSEREW
                     LATSDKPGSRYSGDRDLENPLAAVQMGLIYVNPEGPDGNPDPVASGRDVRVTFARMAM
                     NDEETVALVAGGHTFGKAHGAGDPKLIGPEPEAAPLETQGLGWINKLGTGKGVHTTTS
                     GIEGAWKPKPTTWDNGYFDMLFGYEWELVKSPAGAWQWLAKDVKPEHMIPDAHDPSKK
                     HRPMMTTADLSLRFDPIYEPISRRFHKDPQAFADAFARAWFKLTHRDMGPKARYLGPE
                     VPQEDLIWQDPVPAVDHPLIDAADIAGLKAKILASGLSVSELVSTAWASASTFRGSDK
                     RGGANGARIRFAPQKDWAVNQPAQLARVLAALEAIQKEFNSAQKDGKKVSLADLIVLG
                     GCAAVEAAAKAGGHAVEVPFTPGRMDASREQTDVDSFTVLEPKVDGFRNYHPKAYAHC
                     AEHLLVDKAQLLNLSAPEMTVLIGGLRVLGANADGSRHGLFTTRAGVLSNDFFVNLLD
                     MGTEWKSLSEAEGLYEGVDRKSGQRKWSGTRVDLVLGANSQLRAIAEVYAQSDSKEKF
                     VRDFVAAWNKVMNLDRFDLA"
     assembly_gap    4432..4441
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4442..4680)
                     /locus_tag="PKM23_12045"
                     /pseudo
     CDS             complement(4442..4680)
                     /locus_tag="PKM23_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666164.1"
                     /note="has catalase and peroxidase activities;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
BASE COUNT          873 a         1463 c         1468 g          946 t
ORIGIN      
        1 ccgggaaaat ggctaataat acctatccat ctgaccagag ccattcacct tccaggacag
       61 agcagcaaaa tgaaaaagat ttttctcgca cttcttctct tctcctccct cacccgtgcc
      121 gccatttatg tctccccgga cggcagcgat ctcaatccgg gcacgctgga gaagcccctc
      181 aagaccatcc ccaaggcggt catcaaggcc gcggggggtg acaccatctt tctccgcgcc
      241 ggcatccatg agctgaccgc caatgtcacc atcggcgaca ccaagagcgg cacggaaatc
      301 aaaagatcct gcctgtgggg ctatccgggc gaacgtccgc tgatcgactg ctcgaaagag
      361 gccctcggca gccgggcctt taccctcaag gcgagctggt ggcatttccg ggatttcgat
      421 ctcaagggcg ccggagacaa cggcctgcag atcaccagcg tcacaggcgc gtacaacctg
      481 atcgaaaatt gcactttttt cgaatgccgc gactccggca tccaactctc cgccggcgcc
      541 caccacaacc gcatcatcaa ctgcgactct taccataacg ccgacccgcc cgactatggc
      601 gacgccgacg gcttcgcccc gaaactcgac gtcggcagcg ataactattt ttatggctgc
      661 cgcgcctggg gcaactgtga cgatggctgg gacggctata tgcgcggcgc caccaatgtt
      721 accaccatcc tcgagaactg ctggacctgg ggcaacggct ggctcaagga cggcagcgat
      781 ccaggctccc aggccaatgg caacggcttc aagatgggcg ggggcgacaa cagcaaccgc
      841 gacatgctca tgcaccattt catcctcacc cactgcctcg cctttaactg caaggccaag
      901 ggcttcgacc agaataacaa cgtcggctcg atgacccttt acaactgcac cgggtatggc
      961 aatgggaccg ctaattaccg catcaccaaa cccctggcgg ccggtcagac cctgacggtc
     1021 aagaactgcc tctccttcaa cggtggcgcc gagttgggca gcttcgccgt ccaggaggcc
     1081 aacagctggc tcgcgccttt tgtggtcacg gcggcggatt ttgtcggcgt cgattatgcc
     1141 ctggccgccg caccgcgcaa ggccgacggc tccctgccgg atatcccctt catgcatctc
     1201 gctctcggca gcgatctgat tgacggcggc gtcggtctcg ggctaccctt tctcggcgcc
     1261 gctcccgatc ttggggcctt tgagtccgat taccgatcca tggtaaccag cctcgaagca
     1321 gcgcccgaag gattccggct ggagcagaat tatcccaatc ccttcaatcc ggcgacgacg
     1381 atcgcttacg ccctgccgca gccggagcgg gtcagtctgc gcatctataa cttgcagggg
     1441 caggaggtcg ccagcctcgt cgaaggctgg catccggcgg gcgaacacag ggtggtctgg
     1501 cggccgcagg gcctggccgc cggcatctac atcgcccggc tgcaggcggg gatctttaat
     1561 gcaacccggc ggctggtgct ggccaaataa ggaaacccgg aaaacaaaaa gcccggaata
     1621 ccactggtat cccgggcctt tttataagca gtgatgagta actacttctt cttcaggatg
     1681 gtgctcagca ccagctcctt ggccagcttt ttggtgcaga agaaccagtc gtaacaggtc
     1741 atatcttttt tgttctccat gcgctccttg gcaacgccgc aggagccggc ggtcgggacg
     1801 atgacatcgt cgccgggacg ccagtcagcc ggcagagcga ccgaaaaagc atcggcggtc
     1861 tgcaggccaa tgacaacgcg atagagctcg tcaaaattgc ggccgagact gagcgggtag
     1921 tagatgatgg tgcggatgat gcctttgggg tcgatgaaga agacggcacg cacagccttg
     1981 gttgtggctt cgcccggctg aatcatgccg tactttttgg ccacttccat ggtgatatcc
     2041 tcgatcagcg ggaaggtcac ctcgacgttt ttcatgccct tgtactcgat cttttccttg
     2101 atggtgcgca gccaggcgat gtggctgtag agtccgtcca ccgagagacc gaccagcttg
     2161 cagttggcct cgttgaactg tttctccatg gttgcaaagg tcatgaactc ggaggtgcaa
     2221 accggcgtaa agtcggcggg atggctgaag agaatcaccc aactgccggc gtactgctcc
     2281 ggaaaattga tctcgccctg ggtggtgacg gccttgaaag cgggggcctt gtcgccaatg
     2341 cggggcatgg aaacgacttg ttcgtccatc tggttttcct ttcatgattg tgaagtgaag
     2401 gtggctggct gccgggacta ccggcagctg ttattaaatc aattcactct gctgaaaatc
     2461 gcgcatcaat ccggccgcag cgtcgccggc tgcactcaag cgaggtcgaa gcggtccaga
     2521 ttcatcacct tgttccaggc cgcgacgaaa tcgcgcacga atttttcctt gctgtcggac
     2581 tgggcataga cctcggcgat ggcgcgcagc tgcgagttgg cgccgaggac caggtccacc
     2641 cgggtgccgc tccatttgcg ctggccgctc ttgcggtcaa cgccctcata gaggccttcc
     2701 gcttcggaga gagacttcca ctccgtaccc atgtcgagca ggttgacaaa aaagtcgttg
     2761 ctgagcacac cagcccgcgt ggtgaagagg ccgtgcctgg agccgtccgc attggcgccc
     2821 aggacgcgca ggccgccgat gaggacggtc atctccgggg cgctcaggtt caggagctgg
     2881 gccttgtcga cgagcagatg ctccgcgcag tgcgcatagg ccttgggatg gtaattgcgg
     2941 aaaccgtcca ccttcggttc cagcaccgta aaggagtcca catcggtctg ctcccgggag
     3001 gcgtccatgc ggccgggcgt gaagggcacc tcgacggcgt ggccgccggc tttggccgcg
     3061 gcttccacgg cggcgcagcc gcccagcaca atcagatcgg cgagggagac ctttttgccg
     3121 tctttctgcg cactgttgaa ctctttctga atggcttcaa gcgcggcgag caccctggcc
     3181 agctgcgccg gctggttgac cgcccaatcc ttctgcggcg cgaagcggat gcgggcgccg
     3241 ttggcgccgc cgcgcttgtc ggaaccgcgg aaggtggagg ccgaggccca ggcggtggag
     3301 accagctcgg acacgctgag accggaggcg agaattttcg ccttgaggcc ggcgatgtcg
     3361 gccgcgtcga tcaatgggtg atcaacagcg gggaccgggt cctgccagat cagatcctct
     3421 tgcggcacct cggggccgag gtagcgggcc ttgggtccca tatcgcggtg ggtcagtttg
     3481 aaccaggcgc gggcgaaggc gtcagcgaag gcctgcggat ccttgtggaa acggcgcgag
     3541 atcggctcat agatcgggtc gaagcgcagg gaaaggtcgg cggtggtcat catcgggcgg
     3601 tgcttcttgc tcggatcgtg ggcatccggg atcatgtgtt ccggcttgac atccttggcc
     3661 agccactgcc aggcgccggc cgggctcttg accagctccc attcatagcc aaaaagcata
     3721 tcgaaatagc cgttgtccca ggtagtgggt tttggtttcc aggcgccttc gatgccgctg
     3781 gtggtggtgt ggaccccttt gccggtgccg agtttgttga tccagcccag gccctgcgtc
     3841 tccagggggg cggcttccgg ctcgggcccg atcaatttcg ggtcgccggc gccgtgggcc
     3901 ttgccgaagg tgtggccgcc ggcgacgaga gcgacggtct cctcgtcatt catggccata
     3961 cgcgcgaagg taacacgcac atcccggccc gaggcgacgg gatcgggatt gccgtccggc
     4021 ccctccgggt tgacatagat caatcccatc tgcacggcgg caaggggatt ctccaggtcg
     4081 cggtcgccgc tgtagcggct gccgggcttg tcgctggtcg ccagccactc cctctccgag
     4141 ccccaatagg tctcttcttc cggttgccag atatcctcgc ggccgccgcc aaagccgaag
     4201 gtcttgaagc ccatcgattc gagggcgcag ttgccggcca ggatcatgag gtccgcccag
     4261 gagattttgt tgccatactt ttgcttgatg ggccagagca ggcggcgcgc cttgtcgagg
     4321 ttggcgttgt cgggccagct gttgagggga gcgaagcgct gggtgccatc cgaggctccg
     4381 ccgcgaccat cgctaacgcg ataagtgccc gcactgtgcc aggccattcg annnnnnnnn
     4441 ntggccgtag tccgcgggcc accagtcctg ggatgtagtc atgagggcga cgatgtcctt
     4501 cttcaaggca tccaggtcga gcttcttgaa ttcttcggcg tagttgaagt ccgcacccat
     4561 gggatcactc ttgggcgaat tctgccggag tatctggaga ttcaactgat cgggccacca
     4621 gtcaccggtc gtcattgcgg atgccgccgt gtgtcgactg ggcgctgcgg tggtgtccat
     4681 gacgggacat ttgcccggtt gggcgccgcc ttcggacgcc ggtgccttgt cggcatcctg
     4741 cgccagcgcc gtggtgccat
//
LOCUS       DAOPWU010000276         4759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_18032_length_4759_cov_5.428571, whole genome shotgun sequence.
ACCESSION   DAOPWU010000276 DAOPWU010000000
VERSION     DAOPWU010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18032_length_4759_cov_5.428571"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKM23_12050"
     CDS             complement(<1..257)
                     /locus_tag="PKM23_12050"
                     /inference="COORDINATES: protein motif:HMM:NF015604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HNY92246.1"
                     /translation="MTRISALIILILVTIAAVRAEDGSRLWLRYDRIDDPVLLTACQK
                     NLTGWFIEGDSPVLRAAAGELERGLSGLLGRAIPEARSARR"
     gene            529..798
                     /locus_tag="PKM23_12055"
     CDS             529..798
                     /locus_tag="PKM23_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92247.1"
                     /translation="MRKEWLQKMVTAAEVEAAHMVKDDRLGPDPVPFGFKNERWRAML
                     AQMEEGDELWEFRSPVESWEHLAGRAGIALVRQGEIINFIVTMMN"
     gene            844..1419
                     /locus_tag="PKM23_12060"
     CDS             844..1419
                     /locus_tag="PKM23_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNY92248.1"
                     /translation="MDDAIAFIREADKLKNIVRKTRNFSNERYENDAEHSWHICLMAI
                     TLQPWANEAVDIARVLKMLILHDLGEIFCGDTIVYAKNDDHKRRELEAAHDFFAMLGP
                     LRQEEYNTLVNEFEARATPEARYANAIDRAEPILQNLHHKGETWNKNHISFDRVVALN
                     AALVSEGSRALWEYILRSLEEMRAAHLFSQK"
     gene            1547..2017
                     /locus_tag="PKM23_12065"
     CDS             1547..2017
                     /locus_tag="PKM23_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92249.1"
                     /translation="MDENTCCPEFDPAPWDDKLFEWSDKQFIKDKVRTLFFMSLNFGS
                     VMKRLDARVTAAGGTFAGGLCLSDHTSRWNMDVYLAVDREIPGAENTTLSGKFLTRVY
                     EGPFQDTGKWCADFSAKAGEQGLEVKKWFMWYTTCPKCAKKYGKNYTVIVGQVE"
     gene            2082..2921
                     /locus_tag="PKM23_12070"
     CDS             2082..2921
                     /locus_tag="PKM23_12070"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HNY92250.1"
                     /translation="MRRTRICRLLSWMLVAVIISAGPGCSPALKKGGPTAIDPRPKVI
                     LGIFGGMGPEATASLYQLIVERTPATRDQEHIPTLIYSLPTVPDRTASIFSGDPSILP
                     YLAEGVTRLEKSGASYIVIPCNTAHYFYDHMQGAVKIPIINMIREAAEEVAVKYPGVK
                     KVGLLATTGTISTGIYEKEFAARGIEMIKPSASILENYVMKAVYLIKAHGDKKQCEEW
                     LYLAGKELEKSGAEVVVLGCTEIPLAFNPERSGVPVVSATQVLADRAIARFQQLSIFQ
                     PVQ"
     gene            complement(2977..>4759)
                     /locus_tag="PKM23_12075"
     CDS             complement(2977..>4759)
                     /locus_tag="PKM23_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNY92251.1"
                     /translation="GGDTLRMRFSNEFSSAPVTLQRVLIAASAGGGVIDPATERPLLF
                     QGGNTVTIPPGLAATSDPFPFPLQPRMELAVTIHFGSTPSGLTGHPGSRTTSYLLAGD
                     QTGRADFAGAVRTDHWYIIHTIDVQAPAPAAAVAVLGNSITDGRGSGTNKQNRWPDEL
                     ARRLQENPETQHVAVLNQGIGGNCVLGTCLGPSALSRFNRDVLEQSGVRWLVILEGIN
                     DIGNGGSGVGERLIDAFSQMIRSAHSRGIYVYGATLLPMSGNSYYSPIHEAERQKVNQ
                     WIRHGGLFDGVIDFDLALRNPADTLSLLPEADTGDHLHPNETGHRLMGEAVDLSLFTG
                     REPYVSKERSESRFHEPECATVGGNWRIGTDSQASGGVYVMVEAGMQSLSAAPAGGEN
                     WIQLPFTVDSSATFSLFARLNCPSADDDSYWIKMDGGGFQMFNGLGTNGWEWKKLGDY
                     ALTAGAHTLVIGYREDGAKLDKICLSNAGFAPTGVGEEAENSCTTTGAQAPAEIPTGC
                     TLGQNYPNPLNPATLIRYQLPGRTGVRMEVWDAAGRRVSTLIDRVQESGEHTIRFDGS
                     GLASGVYFYRLTTSTGFSQSRRMLLLR"
BASE COUNT          980 a         1444 c         1470 g          865 t
ORIGIN      
        1 tggcgtctgg cggaacgcgc ctccgggatg gcccggccca ggagcccgct caagccgcgc
       61 tccagctcac cggcggcagc ccgcagcacg ggcgagtcgc cctcgatgaa ccagccggtg
      121 agattttttt gacaggcggt taggagaacc ggatcgtcga tccggtcata gcgcagccag
      181 agccggctgc catcctcagc gcgcacggct gcaattgtca ccaaaatgag tatgatcagg
      241 gcggaaatgc gggtcatgtt attcatcaca ggcagcctcg gcataaatac aactgaatcc
      301 tttccaaatt acccaatcgc gcatccaaaa tcaaggagtt ttgcggcagt gcagcccccg
      361 aaatcaggct cctgcctggt gccgcgtccc ccctcttcat gccggccgca gccaatctgc
      421 catgctccac cgcagcgcag ggattgtttt gtcttgcatg aaacctctct tttttgtaac
      481 gtacataaag agtatcaacg agaaattaac ccttcgtcga ggtggaatgt gagaaaagaa
      541 tggctgcaaa agatggtgac ggccgccgag gtggaggccg cacacatggt caaggacgac
      601 cggttggggc ccgatcctgt gccctttggc ttcaagaacg agcgctggcg cgccatgctg
      661 gcgcagatgg aggaggggga cgaattatgg gagttccgca gcccggtgga gagctgggaa
      721 cacctcgccg gacgcgccgg gatcgccctg gtccgccagg gtgagatcat caattttatt
      781 gtcaccatga tgaactaggc cgtcctgcgc ggtatgcggc aagcgaagga gagtgtgacc
      841 cggatggatg atgcaatcgc ctttatcagg gaggcggaca aactcaagaa tatcgtccgc
      901 aagacgcgaa atttttccaa cgagcgctac gaaaacgatg ccgagcattc ctggcacatc
      961 tgcctcatgg ccatcaccct ccagccctgg gccaacgagg cggtcgacat cgccagggtg
     1021 ctgaaaatgc tcatcctcca cgatctgggg gagatctttt gcggagacac catcgtctac
     1081 gccaaaaacg atgaccacaa gcgcagagag ctggaggcgg cgcatgattt cttcgccatg
     1141 ctgggtccgc tccgccagga ggagtataac acccttgtga acgaattcga ggcccgcgca
     1201 acccccgagg cccgttacgc aaatgccatc gaccgtgcgg agccgatcct gcagaatttg
     1261 catcacaaag gggaaacctg gaacaaaaac cacatctcct tcgaccgggt ggtggcgctg
     1321 aatgctgcgc tggtctccga ggggtccaga gccctctggg aatacatcct ccgcagcctc
     1381 gaagagatga gagcggcgca tctcttcagc caaaaatagc agagttcccc ggtcagctgc
     1441 cagcgcaaag ccggcggaat gccgcgcctg caccgcaagg aaacagggcc tcttgctgtc
     1501 aaaagaccgc aactttttca gtgattactg acttatggag gaagccatgg atgagaatac
     1561 ctgctgcccg gagttcgatc cggcgccctg ggatgacaag ctgttcgaat ggtcggacaa
     1621 gcaattcatc aaggacaagg tccgcaccct ctttttcatg tcgctcaatt tcggctccgt
     1681 catgaagcgg ctggatgcca gggtgacggc tgcgggcggc accttcgccg gcgggctctg
     1741 cctttccgat cacacctccc gttggaacat ggacgtctat ctcgccgtgg atcgcgagat
     1801 tccgggtgcg gaaaacacca ccctgagcgg gaaatttctg accagggtct acgaaggacc
     1861 cttccaggat accggaaaat ggtgcgcgga tttcagcgca aaggctggag agcaggggtt
     1921 ggaggtcaag aaatggttta tgtggtatac tacctgcccg aaatgcgcaa agaaatacgg
     1981 caaaaactac accgtcatcg tcggccaggt ggagtaatcc tgcaaggggc cggccccggc
     2041 ggaccaacag aggctctttc gttcaatcaa ggagaggaaa aatgaggcgt acgagaatct
     2101 gcaggctgct gtcctggatg cttgtggcgg tcatcatcag tgcggggccc ggctgttccc
     2161 cggctctgaa aaagggcggc ccaaccgcca tcgatccccg gccgaaggtc atcctcggca
     2221 ttttcggcgg catggggccc gaagccacgg ccagcctcta tcagctcatc gtcgagcgca
     2281 ccccggcgac cagggaccag gagcacatcc ccaccctcat ctacagcctg ccgacggtgc
     2341 ccgaccgcac cgcctccatc ttcagcggcg acccctccat cctcccctat ctggccgaag
     2401 gggtgacccg actggaaaaa tccggggcct cgtatatcgt catcccctgc aacaccgccc
     2461 actatttcta tgatcacatg cagggtgcgg tgaaaatccc catcatcaac atgatccgcg
     2521 aggctgcaga ggaggtggcg gtcaaatatc ccggggtcaa aaaggtggga ctgctggcga
     2581 cgacggggac catctccacc ggcatctatg agaaggaatt cgccgcgcgc ggcatcgaga
     2641 tgatcaaacc ctccgccagc atcctggaaa actatgtcat gaaagcggtc tacctgatca
     2701 aggcgcacgg cgacaaaaaa cagtgcgaag agtggcttta cctggccggc aaggagctcg
     2761 agaagagcgg ggccgaggtg gtggtgctcg gctgcaccga gatccctctc gccttcaatc
     2821 ccgagcgctc cggcgtgccg gtggtgagcg ccacccaggt gctggcggac cgggccatcg
     2881 cccgctttca gcagctctcc attttccagc cggtgcaata gccggggcag ccggcaaggc
     2941 cgctgagcac aggccggccc cgcccagcag gcgccctcac ctgagcagca gcatgcgtct
     3001 gctctggctg aaacccgttg aggtggtgag ccggtagaaa tagacgccgc tggcaaggcc
     3061 cgagccgtca aagcggatgg tgtgttcgcc gctctcctga acgcgatcga tcagcgtgct
     3121 gacccgtctg ccggcggcat cccatacctc catgcgcacg ccggtccgtc cgggcagctg
     3181 atagcggata agagtggcgg ggttgagggg attcggatag ttctgcccca aggtgcagcc
     3241 ggtcgggatc tcggcggggg cttgtgcgcc ggtcgtggta cagctgtttt cagcttcctc
     3301 ccccaccccc gtcggcgcaa aaccggcgtt ggagagacag atcttgtcga gtttggcgcc
     3361 gtcctcgcga tagccgatga cgagggtgtg cgccccggcg gtgagggcat agtcgccgag
     3421 ctttttccac tcccatccgt tcgtccccaa tccattgaac atctggaatc ccccgccatc
     3481 catcttgatc cagtaggaat catcatcggc gctcggacag ttgagccggg cgaagagaga
     3541 aaaggttgcg gagctgtcga ccgtaaaggg gagctggatc caattttcgc cgccggccgg
     3601 ggcggcgctc aggctctgca ttcccgcctc aaccatcaca tagactcccc ccgaggcctg
     3661 ggaatcggtg ccgatccgcc agtttccacc cactgtcgcg cactcgggct catggaaacg
     3721 cgactcgctc cgttctttcg agacataggg ttccctgccg gtgaaaaggc tcagatccac
     3781 agcctcgccc atcaggcggt ggccggtctc attgggatga agatgatccc cggtatccgc
     3841 ctccggaagg aggctgaggg tgtcggccgg attgcgcagg gcaaggtcga aatcgatgac
     3901 cccgtcaaaa agcccgccat gccggatcca ctgattgacc ttttgtctct ccgcctcgtg
     3961 gatgggcgaa tagtaggagt taccagacat ggggagcagg gtggctccat agacatagat
     4021 gccgcgggag tgggcgctgc ggatcatctg ggaaaaggca tcgatgaggc gctcgccgac
     4081 cccgctgcct ccgttgccga tatcgttgat gccctcgagg atgaccagcc agcgcactcc
     4141 gctctgttcc agcacatcgc ggttgaagcg gctcaaagcg gagggcccca ggcaggtccc
     4201 gagcacgcag ttgccgccga tcccctgatt gagaacggcg acgtgctgcg tctccggatt
     4261 ctcctggagc cgccgggcca gttcatccgg ccagcggttc tgtttgttgg tgcccgatcc
     4321 gcgtccgtcg gtgatggaat tgcccagcac ggccaccgct gccgcgggag cgggggcctg
     4381 gacgtcgatg gtatggatga tgtaccaatg gtcggtccgc acagcgcccg cgaaatcggc
     4441 cctgccggtc tggtcgccgg cgagcagata ggaggtggtc ctggagccgg gatggccggt
     4501 caggccggag ggggtgctgc cgaaatggat ggtgacggcg agttccatcc gcggctgcag
     4561 gggaaatggg aagggatccg aggtggccgc caagcccggc gggatggtca ccgtgttccc
     4621 gccctgaaag agcagcggcc gctctgtggc tggatcgatc accccgcctc ccgccgaggc
     4681 ggcgatcaga accctttgca gggttaccgg ggcggagctg aactcattgc tgaaacgcat
     4741 gcgcagggtg tcgccgccg
//
LOCUS       DAOPWU010000277         4720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_18234_length_4720_cov_5.127974, whole genome shotgun sequence.
ACCESSION   DAOPWU010000277 DAOPWU010000000
VERSION     DAOPWU010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18234_length_4720_cov_5.127974"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PKM23_12080"
     CDS             complement(<1..550)
                     /locus_tag="PKM23_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNY92252.1"
                     /translation="MKYDIKDIDLAEGGKKRIEWAEHDMPVLRQVRERFEKEKPLAGK
                     KLSACLHVTAETANLMRTLKAGGADLVLCASNPLSTQDDVAAALVKEYGIAVFAIKGE
                     DDKVYYDHIHAAIEHGPVITMDDGADLVSNIHFDYPEVARSVIGSMEETTTGVIRLRA
                     MAKDGALKFPVIAVNDATTKNLF"
     gene            complement(647..1783)
                     /gene="metK"
                     /locus_tag="PKM23_12085"
     CDS             complement(647..1783)
                     /gene="metK"
                     /locus_tag="PKM23_12085"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNY92253.1"
                     /translation="MPEVLFTSESVTEGHPDKVADQISDAVLDAVFKDDPRGRVACET
                     YVTTGLCLVGGEITTETYVDIPAIARETIREIGYTNADFGFDYKTCAVITTIDQQSTD
                     IAMGVDREGAGDQGMMFGFAIDETPELMPLPIVLAHRLTRRLAEVRKQGVLPYLRPDG
                     KSQVTVNYVDGKPAYVETVVISSQHDPDVSQKQIHEDVIARVIEPILPKEMIPAHGVI
                     YHVNPTGRFVIGGPQGDTGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSASYAAR
                     YIAKNVVAAGLARKCEIQLAYAIGVAEPVSVMVDAFGTGSMPEEEIVKLIRKNFILTP
                     RGIIEMLDLRRPIYKATASYGHFGRDDQDFPWERTDKAAVLARG"
     gene            complement(1882..3690)
                     /gene="ptsP"
                     /locus_tag="PKM23_12090"
     CDS             complement(1882..3690)
                     /gene="ptsP"
                     /locus_tag="PKM23_12090"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNY92254.1"
                     /translation="MHAESAASQEMRIKGLAVSQGIAIGPAFPFGSGIQDVRPHEVAA
                     AEVEAEVQRLTQAIENAKSELTRIGEETQERLGERSAEIFEVHILILDDPEIRGAAIR
                     RIREERLNAEWLFLQVVEKYEQSFSHFKDEYMRARATDLQDVKRRVIRQLQGAAKSPG
                     DGLPGPAVVFAADLMPSETIHLRRDYVLGFATELGSRTSHAAILARSLKVPSVVGLGP
                     VCQYLVPGTPVILDGYSGQLILNPAPETLERYEKTRSLYEALSHKLARSRTLPARTKD
                     GKDIELSANIEFFEEVQPAIADGAIGVGLYRTEYLFLMRDELPTEEEQYAEYVRIIDA
                     LHRQPIIFRTFDLGGDKAQRHFHVHQEANPFLGYRGVRLYSEREEVFRMQLRAILRAS
                     VRGRVRILIPMISSVTEMRFSRGVLESVKEELRAAAIPFDEEIPVGAMIEVPSAAVSA
                     DLIARECDFLSIGTNDLIQYTLAVDRSNRYVDQMYRAFSPSVLRLIRDTIHHGHAQGV
                     WVGMCGEMASDPLATMALIGMGLDEFSVSPVSLLVIKDIIRRVHFDECENLAERVLSY
                     STADEVENYLSSVFKRKFRDLIIHDPNHPLVKREQN"
     gene            complement(3712..3981)
                     /locus_tag="PKM23_12095"
     CDS             complement(3712..3981)
                     /locus_tag="PKM23_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNY92255.1"
                     /translation="MIEKKFRIKNKLGIHARPAGQLVKVATRFKSAIYISRNGNEVNA
                     KSIMGVLMLEAGHGSEVIIRINGEDEFSALEAIEELIDKRQFDEE"
     gene            complement(4031..>4720)
                     /locus_tag="PKM23_12100"
     CDS             complement(4031..>4720)
                     /locus_tag="PKM23_12100"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HNY92256.1"
                     /translation="FGFSAIPFIKRLFKTQRQREECLRRHLDFFNSHPYYASWCLGAV
                     AKLEEEAKRKKWDDSRPIEIFKERLIGPLGAIGDRLFWDGIKPAAAGAGIALALTAGW
                     IAIPVFLLLYNIPHLAVRIYGLREGYRQGFDIVSGLSVRRYQRWFDVMMMAGGVIAGL
                     VLAAAVRWSWLQGAGRMDPLHAESFVPPALFAGALLLTWVALHFRISRHWTIAGAVLL
                     GIIISLAAAAL"
BASE COUNT          895 a         1479 c         1446 g          900 t
ORIGIN      
        1 cgaagaggtt cttggtggtg gcgtcgttga ccgctatgac cgggaatttc agggccccgt
       61 ccttggccat ggcgcgcagg cggatgacgc cggtggtggt ctcctccatg ctgccgatca
      121 ccgacctggc tacctcggga taatcaaagt ggatattgga gaccagatcg gcgccgtcat
      181 ccatggtgat gaccgggcca tgctcgatgg ccgcgtggat atggtcatag taaaccttgt
      241 catcctcgcc cttgatggcg aagacggcga tgccgtactc cttgaccagg gctgcggcga
      301 cgtcatcctg ggtcgagagg gggttggagg cgcatagcac cagatccgcc ccgcccgcct
      361 tgagggtgcg catcagattg gcggtctcgg cggtgacatg caaacaggcc gagagtttct
      421 tccccgccag gggcttttcc ttctcgaacc gctccctcac ctggcgcagc accggcatgt
      481 catgctcggc ccactcaatg cgcttcttcc cgccctcggc gagatcaata tccttgatat
      541 cgtatttcac aactcctcca ttttccgggg tatgagacat aaagcccacc ggtccccagg
      601 ggccgatggg ctgcctgtac agggtgaatg gaaatgaggg ctggactcag ccccgcgcca
      661 acacggcggc cttgtccgtg cgctcccagg ggaaatcctg gtcatcgcgg ccgaaatggc
      721 cgtaggaggc ggtcgccttg tagatcggac ggcgcagatc gagcatctcg atgatgccgc
      781 gcggcgtcag aatgaaattc ttgcggatca gcttgacgat ctcctcctca ggcatggaac
      841 cggtcccgaa ggcatcgacc atcaccgaga ccggctcagc cacaccgatg gcataggcga
      901 gctggatctc gcactttctc gccagacccg cagccaccac gttcttggcg atgtagcgcg
      961 ccgcatagga ggcggaacgg tcgaccttgg tgggatcctt gcccgaaaag gccccgccgc
     1021 cgtgacgggc ataaccgccg taggtgtcga cgatgatctt gcggccggtc agaccggtgt
     1081 cgccctgcgg gccgccgatg acaaatcgcc cggtcgggtt gacgtggtaa atcacgccgt
     1141 gagcggggat catctctttg ggcaggatgg gctcaatcac ccgggcgatg acgtcttcat
     1201 gaatctgctt ctggctgaca tcggggtcat gctggctgga gatgaccacg gtctcgacat
     1261 aggccggctt gccgtcgacg taattgaccg tgacctggct cttgccgtcg gggcgcagat
     1321 aaggcagcac cccttgcttg cgcacctcgg ccagcctgcg cgtcaaacgg tgggccagca
     1381 cgatgggcag gggcatcagc tccggggtct cgtcaatagc gaaaccgaac atcatcccct
     1441 ggtcaccggc gccctcgcgg tcgacgccca tggcgatgtc ggtcgactgc tgatcgatag
     1501 tggtgatgac cgcacaggtc ttgtaatcga aaccgaaatc ggcattggtg tagccgatct
     1561 cacggatggt ctcgcgtgcg atcgcgggaa tgtcgacata ggtttccgtt gtgatctcgc
     1621 cgcccaccag gcaaagcccg gtggtgacat aggtctcgca ggcgacacgc ccgcggggat
     1681 catccttgaa tactgcatcc agcactgcat cggatatctg atcggccacc ttgtccggat
     1741 ggccctctgt aaccgactcc gaagtgaata gaacttccgg catgaactga ctctccttgc
     1801 tatatcaatg gatatgggat taattactct gtattatgca acataccgtc acaactgcgg
     1861 ctttgcggat gacaaaaccg ctcaattctg ctcccgcttg accagtggat ggttgggatc
     1921 gtggatgatc aaatcgcgga acttgcgttt gaaaaccgag ctcaaataat tctcgacctc
     1981 gtcggccgta ctgtagctca gcacccgctc ggccagattc tcgcactcgt cgaaatggac
     2041 ccggcggatg atatccttga tcaccagcag cgagaccggg ctgacgctga attcatcgag
     2101 ccccatgccg atcagggcca tggtggccag gggatcgctc gccatctcgc cgcacatgcc
     2161 gacccacacc ccctgggcat ggccgtggtg gatggtgtcg cggatcagcc tcagcaccga
     2221 cgggctgaag gcgcggtaca tctggtcgac gtagcggttg ctgcggtcga ccgcgagggt
     2281 gtactgaatg aggtcgttgg tgccgatgct gagaaaatcg cactcccgcg caatgaggtc
     2341 cgccgagacc gcggcggagg gaacctcgat catcgccccg accgggatct cctcgtcaaa
     2401 ggggatggct gcggcgcgca gctcttcctt gaccgactcg agtaccccac ggctgaagcg
     2461 catctccgtg acgctcgaga tcatcgggat gaggatccgc acccggccgc gcacgctggc
     2521 gcgcaggatc gcccgcagct gcatgcgaaa gacctcctcg cgctcactgt aaaggcgcac
     2581 cccgcgatag ccgagaaagg ggttggcctc ctggtggacg tggaaatggc gctgggcctt
     2641 gtcgcccccg aggtcaaagg tgcggaagat gatcggctgg cgatggagag cgtcgataat
     2701 ccgcacatac tccgcgtact gctcctcctc ggtcggcagt tcgtcgcgca tcagaaagag
     2761 atattcggtg cggtagagcc cgacgccgat cgccccgtcg gcgatagccg gctgaacctc
     2821 ctcgaaaaac tcgatgttgg cggagagctc gatgtccttg ccatccttgg tccgggccgg
     2881 cagggtgcgg ctgcgcgcca gcttgtgcga gagcgcctcg taaaggctgc gcgtcttttc
     2941 atagcgctcc agggtctcgg gagcaggatt gaggatcagc tgcccggaat agccgtcgag
     3001 gatgaccggg gtgcccggga ccagatactg gcagaccggg ccgaggccga ccacggaggg
     3061 gaccttgagc gagcgcgcca ggatggcggc gtgggaggtg cggctgccca gttcagtggc
     3121 gaagccgagc acataatccc gccgcagatg aatggtctcg gagggcatca gatcggcggc
     3181 gaagaccacc gccggacccg gcaggccgtc gcccggcgac ttggccgccc cctgcagctg
     3241 gcggatgacc cggcgcttga catcctgcag atcggtcgcg cgcgcgcgca tgtactcatc
     3301 cttaaagtgg ctgaaactct gctcgtattt ttccaccacc tgcagaaaaa gccactcggc
     3361 gttcaaccgc tcctcgcgaa tgcggcggat agccgccccg cggatctcgg gatcgtcgag
     3421 gatgaggatg tgcacctcaa aaatctccgc gctgcgctcc ccgagccgct cctgggtctc
     3481 ctcgccgatc cgggtcaact cgctcttggc gttttcgatc gcctgggtga gcctctgcac
     3541 ctcggcctcg acctccgcgg cggccacctc atggggccgg acatcctgga tgccgctgcc
     3601 aaaggggaag gccggaccga tggcgatccc ctgcgagacc gccagcccct tgatcctcat
     3661 ctcctgtgat gctgcactct cagcatgcat agtctgcatc catcgtaagc gctactcttc
     3721 gtcgaactgg cgcttgtcga tcagctcctc gatcgcttcc agcgccgaga attcgtcctc
     3781 gccgttgatg cggatgatca cctcgctgcc gtggcccgct tcaagcatca ggacacccat
     3841 gatgctcttg gcgttgactt cgttgccgtt cctggagata tagatcgccg acttgaagcg
     3901 ggtggccacc ttgaccagtt gtcctgctgg ccgggcgtgg atgcccagct tgttcttgat
     3961 cctgaatttt ttttcgatca tctggttctt gctcttgatc agtaataatc agccgctgca
     4021 cccccgcgcc ctagagcgcg gcagccgcca gcgaaatgat aatgccgaga aggaccgccc
     4081 ccgcgatggt ccagtgtcgg gaaatgcgaa aatggagcgc cacccaggtc agcagcagcg
     4141 ctccggcaaa gagcgccggc gggacgaaac tttccgcatg gagcggatcc atccgccccg
     4201 cgccctgcag ccacgaccag cgtactgcag cggccaggac gagtccggcg atgaccccgc
     4261 cagccatcat catgacatca aaccagcgct gataacggcg caccgagagg cctgagacga
     4321 tgtcgaatcc ctgacgataa ccctcgcgca ggccgtagat gcgcacggcg agatgaggaa
     4381 tgttgtaaag gagcagaaaa accgggatag cgatccagcc cgcggtcagg gccagggcaa
     4441 tccccgcccc cgccgcggcc ggcttgatgc cgtcccagaa gaggcggtcg ccgatggccc
     4501 cgagcggccc gatcagacgc tccttgaaga tctcgatcgg ccggctgtca tcccattttt
     4561 tgcgcttggc ctcctcctcg agtttggcca ccgcaccgag acaccagctc gcgtaatagg
     4621 gatggctgtt gaaaaaatcg agatggcggc gcaggcactc ctcgcgctgc ctctgcgtct
     4681 tgaacaaccg cttgatgaag gggatcgctg aaaaaccgaa
//
LOCUS       DAOPWU010000278         4685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_18398_length_4685_cov_4.872138, whole genome shotgun sequence.
ACCESSION   DAOPWU010000278 DAOPWU010000000
VERSION     DAOPWU010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18398_length_4685_cov_4.872138"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKM23_12105"
     CDS             complement(<1..484)
                     /locus_tag="PKM23_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92257.1"
                     /translation="MHKLLTRLLLAAMLLPVLPAMSQDNSGRTSCLFRLDYHYGEINP
                     WLFYERPLAKNVGLSAVFQMSSQGYAQIDLGPAFHIGNLALIPMAGVEFVEAGNKGAR
                     VGHICPEFFVFYNSPQWTFESQNLYFVKVLDETYSNRYYYRHYLGHRLYGDSFIGPQV
                     E"
     gene            complement(599..1243)
                     /locus_tag="PKM23_12110"
     CDS             complement(599..1243)
                     /locus_tag="PKM23_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HNY92258.1"
                     /translation="MKIKTLVPILVLIGIAGCSTLYFAGMEKIGIQKREIMVDRVKAA
                     RDTQKEAKEQFVNALEQFKSVVAVQGGDLEKKYNQLSATLERSEAEAEAVRSRIKSVE
                     EVSTALFAEWKKEIAQYNSETLRRSSQQKYDAAEEKYAQLMKAMKKAESRMEPALVPL
                     RDQVLYLKHNLNAKAIAGLSDEVVTVETRVDKLVADIEAAVAEADEFISQLQEE"
     gene            complement(1365..1988)
                     /gene="leuD"
                     /locus_tag="PKM23_12115"
     CDS             complement(1365..1988)
                     /gene="leuD"
                     /locus_tag="PKM23_12115"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNY92259.1"
                     /translation="MEKFTTLTSPYIALPVANIDTDQIIPARFLKVTDKKGLGENLFY
                     DWRYDETGAPLPDFILNRPEGRRARILTAGDNFGCGSSREHAPWALAGYGFRAVISSS
                     FADIFRNNALKNGILPLIVPVDLLAELLGSADEGSGAPLTIDLAAQTLLLPGGRSVSF
                     AIDPFNKSCLLEGVDSLGYLLQHQAQIAAWEANHPPRISTRVKEANM"
     gene            complement(2078..3487)
                     /gene="leuC"
                     /locus_tag="PKM23_12120"
     CDS             complement(2078..3487)
                     /gene="leuC"
                     /locus_tag="PKM23_12120"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNY92260.1"
                     /translation="MSDTPPKTLFAKIWDDHVVSQEPGAPALLYIDLHLVHEVTSPQA
                     FQGLRERGLGVRRPGQTVATVDHSIPTLDPSLPWSDPLAAAQVRRLKENCRDFGISLY
                     GPDHPARGIVHVIGPEMGLTQPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQ
                     TLLQNRPRTMEVVVDGILGAGVTAKDVILALIARIGVGGGTGCVIEYRGSAIRGLDME
                     GRMTLCNMSIEAGARAGMVAPDEVTFDYLAGRPFAPSGEEWEHQLARWRALASDPGAR
                     FDETVELDAAALEPMITFGTNPAMGIAIDAVIPDPAAAEGGDRETLAKALRYMGLAPG
                     RPLLGQKIEAVFIGSCTNGRISDLRAAAGLLRGRHVAPGVRTLVVPGSQAVKRQAEAE
                     GLDRIFTTAGAEWRESGCSMCIAMNGDQIASGEYAVSTSNRNFEGRQGKGGRTLLASP
                     LTAAASAIRGCVADPRELL"
     gene            complement(3480..>4685)
                     /locus_tag="PKM23_12125"
     CDS             complement(3480..>4685)
                     /locus_tag="PKM23_12125"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNY92261.1"
                     /translation="QYHTGKISATQLKELEDRGCPGCGSCSGMFTANSMNCLMEALGM
                     ALPGNGTILAVDPRREELIRRSGAAVMALLKRDLKPLDILTPRAFHNALVLDLAMGGS
                     TNTILHTLAIAHEAGVRIRLEEINTLAERVPYLCKVSPASRTVHMEDVDRAGGISAIL
                     RELARIPGLVDETAVTITGRCLGENIAGADIRDEEVIRPIGNPHAVRGGLALLFGNLA
                     PRGAAVKIGAVAPGMWRHQGPAQVFDSEEAAIAAINGGRIKPGDVVVIRYCGPKGGPG
                     MPEMLSPTSAIMGMGLGESVALITDGRFSGGTRGACIGHVSPEAAAGGPIAALRDGDP
                     ILIDLHRRTLDTLLDEAEIGRRLAELPPFQPRYRRGWLARYTRLVTSAETGAVLEYNQ
                     PEGRETDDE"
BASE COUNT          800 a         1423 c         1591 g          871 t
ORIGIN      
        1 cctccacctg aggtccgatg aaagagtctc cgtaaagacg atggccgaga tagtggcggt
       61 aataatagcg gttcgaatag gtctcgtcca gaaccttgac aaaatagagg ttctggcttt
      121 caaaggtcca ttgcgggctg ttgtaaaaga cgaaaaattc cgggcagata tggccgacac
      181 gggcgccttt gtttcccgcc tcgacaaatt cgacgccggc catggggatg agcgcgagat
      241 tgccgatgtg aaaggcgggg cccagatcga tctgggcgta gccctgactc gacatctgaa
      301 agaccgcgga gaggccgacg tttttagcga gcggcctttc ataaaaaagc cagggattga
      361 tctcgccata atgatagtcg aggcggaaga gacagctggt gcgcccgctg ttatcctggc
      421 tcattgcagg taaaaccgga agcagcatcg ccgccagaag gagtctggtc aataatttgt
      481 gcatggttgt tcccaggttt ggttttgcag tcaaatcaag gcgccgccgg gatcggggtc
      541 gccaatgcct gtgcggtcag agcggtatgg ccttgaacgg ggcctgatca agcgggggct
      601 actcctcctg cagctggctg atgaattcgt cggcctcggc gacggccgcc tcgatatcgg
      661 cgaccagttt atccacccgg gtctcgaccg taaccacctc atcgctcaat ccggcgatcg
      721 ctttggcgtt gagattgtgc ttcagataga gcacctgatc gcgcagcggc accagggccg
      781 gctccatgcg cgattcggcc tttttcatcg ctttcatgag ctgtgcgtat ttttcctcgg
      841 cggcgtcata tttttgctgg ctggagcggc gcagggtctc gctgttgtac tgggcgatct
      901 cctttttcca ttcagcaaag agggcggtcg agacctcctc gaccgatttg atgcggctgc
      961 gcaccgcttc agcctcggcc tcgctgcgct caagggtggc gctcagctgg ttgtactttt
     1021 tctccagatc gccgccttgc acggcgacga cgctcttgaa ctgctcgagg gcgttgacga
     1081 actgctcctt ggcttccttc tgggtgtccc gcgccgcctt gacgcggtcc accatgatct
     1141 cgcgtttctg gatgccgatc ttttccatgc cagcgaagta gagggtgctg caccccgcga
     1201 taccgatcag tacgaggatg gggacgaggg tcttgatttt catgggatct cctggcaaat
     1261 ggtcattctg ttgacagctt aatataccaa aagaatgacc attgtcaaaa cagaaatatc
     1321 gggggcgccg ccggagcggt gaccaaccgc gcgccggccg gcccttacat gtttgcctct
     1381 ttgacccgtg tcgagatgcg cggaggatgg ttcgcctccc aggcggcgat ctgcgcctgg
     1441 tgctggagca ggtagccgag tgaatcgacc ccctcgagca ggcagctttt gttgaagggg
     1501 tcgatggcaa agctgacgct gcggccgccg gggagcagca gggtctgggc ggcgagatcg
     1561 atggtgaggg gagcgccgct cccttcgtct gcgctgccga gaagctccgc caggagatcc
     1621 accggcacaa tcaggggaag gatgccattc ttgagggcgt tgttgcgaaa gatgtcggcg
     1681 aaactgctgc tgatgacggc gcggaagccg taaccggcca gagcccaggg ggcgtgttcg
     1741 cggctcgaac cgcagccaaa gttgtcaccg gcggtgagga tgcgggcgcg ccgcccttcc
     1801 gggcggttga ggatgaaatc gggcaggggc gctccggttt cgtcgtagcg ccaatcataa
     1861 aagaggttct cgcccagccc cttcttgtcg gtcaccttga gaaagcgcgc ggggatgatc
     1921 tggtcggtat cgatattcgc gaccggcagg gcgatgtagg ggctggtcag ggtggtgaat
     1981 ttttccatgg cactatctcc ggaagcaggt tcaaggcgcg cgggcgggta gatgctgccg
     2041 ctcgaacgcg ccgggttggg gagcgccgcg gcggcgctca gagcagctcg cgcggatcgg
     2101 caacgcagcc gcggatggcg ctggcggcgg cggtgagggg gctggccagg agggtccgcc
     2161 cgcccttgcc ctgccgcccc tcaaaattgc ggttgctggt gctgacggcg tactctccgg
     2221 aggcgatctg atctccgttc atggcgatgc acatgctgca gcccgattcg cgccattcgg
     2281 ctccggcggt cgtgaaaatt cggtccagcc cctcggcctc ggcctggcgc ttgaccgcct
     2341 gcgagcccgg cactaccagg gtgcgaaccc ccggggcgac gtgacggccc ctgagcaggc
     2401 cggctgcagc gcgcaggtcg ctgatgcgtc cgttggtgca ggagccgatg aaaacggcct
     2461 cgatcttttg gccgaggagc ggccggcctg gcgccagccc catatagcgc agggccttgg
     2521 cgagggtctc acgatctcca ccctcggctg cggcgggatc ggggatgact gcgtcgatgg
     2581 cgatccccat ggccggattg gtgccgaagg tgatcatcgg ctcgagggcg gctgcatcga
     2641 gctcgacggt ctcatcgaaa cgagcgccgg gatcgctggc cagcgcccgc cagcgggcca
     2701 gctggtgctc ccactcctcg cccgacgggg cgaagggccg gcccgccaga tagtcgaaag
     2761 tgacctcatc gggggcgacc atgccggcgc gggcaccggc ttcaatgctc atgttgcaca
     2821 gggtcatccg cccctccatg tcgaggccgc ggatagcgct gccgcgatat tcaatgacac
     2881 agccggtccc gcccccgacc ccgatccggg cgatgagggc gaggatgaca tccttggccg
     2941 tcacccctgc gcccaggatg ccatcgacca ccacttccat ggtgcgcggc cggttctgga
     3001 gcagggtctg ggtcgccagc acatgctcga cctcgctggt gccgatgccg aaagcgaggg
     3061 ccccgaaggc gccgtgggtg gcggtgtggc tgtccccgca gacgatggtc atccccggct
     3121 gggtgagccc catctccggg ccgatgacat gcacaatccc gcgcgccgga tggtcggggc
     3181 cgtagaggga gatgccgaaa tcacgacagt tctccttgag gcggcggact tgcgccgccg
     3241 ccaggggatc gctccagggc agtgagggat cgagggtcgg gatggagtgg tccaccgtcg
     3301 cgacggtctg cccgggccgg cgcacgccga ggccgcgctc gcgcagcccc tgaaaggcct
     3361 ggggcgaggt cacttcatga acgagatgga ggtcgatgta gagcagggcc ggagcgccgg
     3421 gctcctggct cacgacgtgg tcatcccaaa ttttggcaaa gagggttttc gggggggtgt
     3481 cactcatcat ctgtttctct tccttcgggt tggttgtatt ccagaacagc gccggtttcg
     3541 gcggaggtga ccagccgggt atagcgcgcc agccagccgc ggcggtagcg aggctgaaag
     3601 ggcggcagct cagcgaggcg ccgtccgatc tcggcctcat ccagcagggt gtcgagggtg
     3661 cggcggtgca agtcgatgag gatgggatcg ccatcgcgca gggcggcgat ggggccgccg
     3721 gccgccgctt cggggctgac gtggccgatg caggccccgc gcgtgcctcc ggaaaagcgc
     3781 ccgtcggtga tcagggccac actctcgccc agccccatcc ccatgatcgc cgaggttggt
     3841 gagagcatct cgggcatgcc cggtcccccc ttggggccgc agtagcggat caccaccaca
     3901 tccccaggct tgatgcggcc gccattgatc gcggcgatgg ccgcctcctc gctatcaaag
     3961 acctgcgccg ggccctggtg gcgccacatc cccggggcca ccgctccgat cttgaccgcc
     4021 gccccgcgcg gggcgagatt tccgaacagg agagccaggc cgccgcggac ggcatggggg
     4081 ttcccgatcg gccgaattac ctcctcatcg cggatatcgg cgccggcgat gttctcacca
     4141 agacaccggc cggtgatggt gacagccgtt tcatccacca gcccggggat gcgggccagt
     4201 tccctgagaa tggcggagat gcctccagcg cgatcgacgt cctccatatg caccgtccgt
     4261 gaagccggcg agaccttgca gaggtagggc acccgttccg ccagggtatt gatctcctcc
     4321 agacggatgc gcaccccggc ttcatgcgcg atagccaggg tgtgaaggat ggtattggtc
     4381 gagccgccca tggccagatc gaggacgagg gcgttgtgaa aggcccgcgg cgtgaggata
     4441 tccaggggct tgagatcgcg cttgagcagc gccatcaccg ccgcgccgct gcggcggatc
     4501 agctcctccc ggcggggatc gacggcgagg atggtgccgt tgcccggcag ggccataccg
     4561 agggcttcca tcaaacagtt catgctgttg gcggtgaaca tccccgagca actgccgcag
     4621 ccgggacagc cgcggtcctc aagttccttg agttgggtgg cggagatttt tccggtgtga
     4681 tactg
//
LOCUS       DAOPWU010000279         4639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_18648_length_4639_cov_4.502618, whole genome shotgun sequence.
ACCESSION   DAOPWU010000279 DAOPWU010000000
VERSION     DAOPWU010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18648_length_4639_cov_4.502618"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="PKM23_12130"
     CDS             <1..1431
                     /locus_tag="PKM23_12130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNY92262.1"
                     /translation="DGVGYGDYLEQLTFLLFLKMADEYSRPPHNRKLPVPQEYNWESL
                     KERSGADLEAHYTKLLSELSRAKGVLGQIFTKSQNKIQDPAKLFKLIDLIDREEWSAM
                     GADVKGRIYEGLLEKNAEDTKSGAGQYFTPRALIRAMVACIRPQPMKTIADPACGTGG
                     FFLAAYDFIAAPENYSLSRDQKEFLKHKAFSGNEIVAGTRRLALMNLFLHNIGDFDSE
                     TFISPADALVADTGVRVDYVLANPPFGKKSSMTFTNDEGEQESDDLTYNRQDFWVTTS
                     NKQLNFVQHIRTMLKSDGRAAVVVPDNVLFEGGAGETVRKKLMATTNLHTILRLPTGV
                     FYKQGVKANVIFFDNKPAAKEPWTREVWIYDFRTNIHFTLKKNPLRFEDLADFIACYC
                     PENLAKRVETWHAETNPEGRWRRFSYDEITARDKTSLDITWIKDQSLTDLDNLPDPDV
                     LAAEIIENLEAGLESFREIYNGLSKQ"
     gene            complement(1658..4465)
                     /locus_tag="PKM23_12135"
     CDS             complement(1658..4465)
                     /locus_tag="PKM23_12135"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNY92263.1"
                     /translation="MPWFDEKSRRFRAVIAVLFTVLFSISALHFYYMIGSPTDENWFT
                     NTPAPFYVIRDLPATPLEVKTGGRFAVKPERVADSLLTGDILLPAGKKTSLQAFSDSL
                     QRLRALDTLLTLTVYRPAFNQVYRYQVASRLQPDTLLRLLPPTVAVFSVARGGASDRA
                     GMKPGDLILSINNKTFKDMFEADSIMRSGRTGKSTVYEVLRRNETLRLDVTLARLGIP
                     FTQLIFMLTGCAYMALGLFLGLSRPQIKAARLLALGFTLLGYVLMLLNTGRDSAPGFL
                     VLVRTYPLPALSFLSIALLMHSTYYFPRPSPLLLARRWPMILPYAAALICSLLVYIRD
                     SGRLFNLLALVLIVISILPLYLFRKQVNPEHKRLSRILRLAMFTTLLLTIALGWYVNR
                     YATPMSAGIIALPLLLIPAAYLYTIGRYRLFDLNLRIRRNIRYSILLTLWILALTIIG
                     ITLFWRLPALPIQLPHITLTGSSIEVVDTPQTQQEQAALVKGLLMVLAVIAVLVLWKI
                     GKLGARFLAKQFHRDQYDYRRASQLLNEVMSRQADLGELAEEVVERVNDLMHLRQIGV
                     LFFRESHEIACGQAAGLPEERWNEFMEEAPALAAKLAAWLPDEERFTIDYLPEKLKKR
                     FFEFGFRYLMPILTQERLRGLFLLGEKNSETPFYQEDYQFLRSISQPVSVAIENALLY
                     EELAQRERLRHEMEIARRIQLASLPQETPHMPGLEIAGLSIPALEVGGDYYEYLNGSS
                     ECLTIVVGDVSGKGISAALYMSKVQGIIRSLHSFHLGPRELCVHLNRLLGHDLEKNYF
                     VTALAAEIRTVERRLVLTRAGHLPLYHYRTRTRSVEAITPRGIGFALREEEHFAQELE
                     EIQVGYERGDIFLFATDGVTECRNPAGEEFGEANLLRLLAGHAQMAAAQLCGALLQEL
                     RHFSGTALQADDLTMVVVRAV"
BASE COUNT          991 a         1300 c         1503 g          845 t
ORIGIN      
        1 gacggcgtcg gctatggcga ctacctcgag cagctcacct ttctcctctt tttgaaaatg
       61 gccgacgagt acagccgtcc gccccacaat cgcaaactcc ctgtgccaca ggaatacaac
      121 tgggagagcc tcaaggagag aagcggcgcc gacctcgagg cgcattacac gaaactgctg
      181 tccgaactct cccgcgccaa aggcgtcctc ggccagatct ttaccaagag ccagaacaag
      241 attcaggatc cagccaagct cttcaagttg atcgatctga tcgaccgtga ggagtggagc
      301 gccatgggcg cggatgtcaa agggcggatt tacgaggggc tgctggagaa gaacgccgag
      361 gataccaaaa gcggcgcggg ccagtacttt actccccgtg cgttgatcag ggccatggtc
      421 gcctgcatcc ggccgcagcc gatgaagacc atcgccgacc ccgcctgcgg caccggcgga
      481 ttcttcctcg ccgcctatga tttcatcgcc gcgccggaga attacagcct gagccgggat
      541 cagaaagagt tcctcaagca caaggccttc tccggcaacg agatcgtcgc cggcacccgc
      601 cgcctggcgc tgatgaacct cttcctccac aacatcggcg atttcgacag cgagaccttt
      661 atctcgccgg ccgacgccct ggtggccgat accggcgtgc gcgtcgatta tgtgctggcc
      721 aatccgccct tcggcaaaaa gagcagcatg accttcacca acgacgaggg cgagcaggag
      781 agcgacgacc tcacctacaa ccgccaggat ttctgggtca ccaccagcaa caagcagctc
      841 aatttcgtcc agcacatccg caccatgctc aaatccgacg gccgcgccgc cgtggtggtg
      901 cccgacaacg tactcttcga gggcggtgct ggcgagaccg tgcgcaaaaa gctcatggcc
      961 acaaccaacc tgcacaccat cctgcgcctg cccaccgggg tcttttacaa gcagggcgtc
     1021 aaggccaatg tgatcttttt cgacaacaaa cctgcagcca aagagccctg gacgcgggag
     1081 gtgtggattt acgatttcag gaccaatatc catttcaccc tgaaaaaaaa tcccctgcgt
     1141 ttcgaagatc tggctgattt catcgcctgc tattgtccgg agaacctcgc caaacgtgtg
     1201 gagacctggc acgccgaaac caatccggag gggcgctggc gccggttcag ctatgacgag
     1261 atcacagcgc gcgacaagac cagcctggat atcacctgga tcaaggacca gagtctgacc
     1321 gatctggata atctgccgga tccggatgta ctggcggcgg agatcatcga gaatctggag
     1381 gcggggctgg agagttttcg ggagatctat aacgggttgt cgaagcagta gtataggcct
     1441 tgcggggatc aatcgtggct gaccgggaat cctcagcgct atatttgagg tccttccgtg
     1501 cagtgagatc atagtctgcc tggctgcgga ctgaatgcat cccgccggcg tacccgcccg
     1561 tccgccgggg gaaaagacga gggcggctca gggctgacgg actcgtacag ttttgaccat
     1621 cgccacggcc agatcgtcga cctggagggc ggcaggctca gaccgccctg accaccacca
     1681 tggtcaagtc gtcggcctgc agggcggtgc cggaaaaatg gcgcagctcc tgcaggagcg
     1741 cgccgcagag ctgcgctgca gccatttggg catggccggc caggagccgc aggagattgg
     1801 cctcgccgaa ctcttctccg gcggggttgc ggcattcggt gacgccatcg gtggcgaaga
     1861 ggaagatgtc ccccctctca tatccgacct ggatctcctc cagttcctgc gcgaaatgct
     1921 cctcctcgcg cagggcgaag ccgattccgc gcggggtgat ggcctcgacg ctgcgggttc
     1981 gggtgcggta gtgatagagt ggtaaatggc cggcgcgggt cagaaccagc cggcgttcga
     2041 cggtgcgaat ctcggcggcc agggcggtga cgaaataatt cttctccaga tcgtggccca
     2101 ggagccggtt gaggtgtacg cagagttcac gcggtccgag atggaagctg tgcagggagc
     2161 ggatgatgcc ctgcaccttg gacatataga gcgcggcgga gatgcctttg ccgctgacat
     2221 cgccgacgac gatggtgagg cattcagagg aaccattcag gtattcataa tagtcgccgc
     2281 ccacttccag ggccggtatc gagaggccgg cgatctccag gcccggcatg tgcggggtct
     2341 cctggggaag cgaggccagc tggatgcgcc gggcgatctc catttcgtgg cgaagccttt
     2401 cgcgctgggc gagttcttcg tagagcaggg cgttctcgat cgcgaccgag accggctggc
     2461 tgatggagcg gagaaactga tagtcctcct gataaaaggg cgtctcggaa tttttctcgc
     2521 cgagcaggaa gagaccgcgc aggcgctcct gggtcaggat gggcatcaga tagcggaagc
     2581 cgaactcgaa gaatcttttt ttcagcttct ccggcagata atcgatggtg aaccgctcct
     2641 cgtccggcag ccaggccgcc agctttgcgg ccagggccgg ggcttcttcc atgaactcgt
     2701 tccatctctc ctcgggcagg cccgcggcct ggccacaggc gatctcgtgc gattcgcgaa
     2761 aaaagagcac gccgatctgg cgcagatgca tgagatcgtt gacccgttcg acaacctcct
     2821 ccgccagttc cccaaggtcc gcctgcctgc tcatcacctc gttgaggagc tgtgaggcgc
     2881 gcctgtaatc atattgatcg cgatggaact gtttggccag gaaacgggcg ccgagtttgc
     2941 cgatcttcca gagcaccaat accgcgatca ctgccagcac catgagcaac cctttgacca
     3001 gtgcagcctg ctcctgctgg gtctgggggg tgtcgaccac ctcgatggag gagccggtca
     3061 gggtgatgtg cggcagctgg atgggcaggg ctggcaggcg ccagaaaagg gtgatgccga
     3121 tgatggtcag cgcgaggatc cacagggtca gcaggattga gtagcggata tttctgcgga
     3181 tgcgcaggtt gagatcgaaa agccggtagc gcccgatggt gtagaggtag gctgccggga
     3241 tgagcagcag ggggagggcg atgatgcccg ccgacatcgg cgtagcatag cggttgacat
     3301 accagccgag agcgatggtc aggagtaaag ttgtgaacat agccagccgg aggattcggg
     3361 agaggcgttt gtgttccgga ttgacctgct tgcggaagag atacaggggc agaatggaga
     3421 ttacgatcaa gacgagggcg agcaggttga aaagacggcc actatcgcgg atgtagacca
     3481 gcaggctgca gatcagggcg gcggcgtagg gcaggatcat gggccagcgc ctggccagca
     3541 gcagggggct gggacggggg aaatagtagg tgctgtgcat caacagcgcg atggagagga
     3601 aggagagagc gggcagaggg taagtgcgga ccaggaccag gaagcccggc gccgaatctc
     3661 gtcctgtatt cagcagcatg agtacgtaac cgagcaaggt gaaacccagc gccagcagcc
     3721 gggccgcctt gatctgcggt cgcgagagtc cgagaaagag gcccagggcc atgtaggcgc
     3781 agccggttag cataaagatc agctgggtga aggggatgcc gaggcgggcg agggtgacgt
     3841 cgagccggag ggtttcattt cggcgcagca cctcgtagac ggtgctcttg ccggtcctgc
     3901 cggagcgcat gatgctatcg gcctcgaaca tatccttgaa ggtcttgttg ttgatggaga
     3961 ggatgagatc gcccggcttc attccggccc ggtccgaggc cccgcccctg gcgacactga
     4021 aaacggccac tgtgggcggc agcagccgga ggagggtgtc cggctgcaga cggcttgcca
     4081 cctgatagcg gtagacctgg ttgaaggccg gccggtagac ggtgagggtg agcagggtat
     4141 cgagggcgcg caggcgctgg agcgagtcag agaaagcttg aaggctggtc ttctttccgg
     4201 cggggaggag gatatcgccg gtgagcaggc tgtcggccac gcgttccggt ttcacagcaa
     4261 agcggccgcc ggtcttgact tcaagcgggg tggcagggag atcccggatg acgtaaaaag
     4321 gggcaggcgt gttggtgaac cagttttcgt cggtcgggga gccgatcatg taataaaaat
     4381 ggagtgcgct gatgctgaag agcactgtaa agagcacggc gatgaccgcg cgaaaacgcc
     4441 gggatttttc atcaaaccag ggcatcggca catcctcact gtgtcagggg acttgcacta
     4501 ctccgtttta cgtctcccat aatataatgt ttccggagca gatcggacag acatttcttt
     4561 cagggactac tggccgatgt cacaagctga ggcccgatat ccagcccctt atgtaaaaac
     4621 actcaattac cataatatc
//
LOCUS       DAOPWU010000280         4598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_18853_length_4598_cov_4.201409, whole genome shotgun sequence.
ACCESSION   DAOPWU010000280 DAOPWU010000000
VERSION     DAOPWU010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18853_length_4598_cov_4.201409"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="PKM23_12140"
     CDS             complement(<1..1267)
                     /locus_tag="PKM23_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNY92264.1"
                     /translation="MNAFFSAQGCYLAGALLALLVGGKRKAVGWVAFGAAAFGTLFIL
                     TAVLGVFREGPVSSGLPILQVPGLGAALIYQIDYLSALFLAIIAVVSLLVTLYAQRFM
                     QIAHYRAGSLRYFYSILLFFFAAVLNVVVVADLFFFFVFWEIMTLTSYLMVIFRQEER
                     VRLRAGYKYFLITHIATIFLFIGGILLYRLSGSFSFSFLKMAMAEMQQSQPGLLHFVL
                     GCFFLGFATKAGILPMGDWLPDAYPAAPAPASAAFAGSMTKLGIYGLVRVFCGLLPLS
                     SHTQIWGLIIALFGAGSILVGTMTALVQEDAKRLLSFHVIGQMGYMFLAIGTGLYFLR
                     TQPVIGMIGLGAGIFHLINHVSYKSCLFFNAGAVFYKTGTRDINKVGGLARVLPVTAA
                     TAIIASLSIAGIPPFSGFASKWLIYQAALR"
     gene            complement(1308..2762)
                     /locus_tag="PKM23_12145"
     CDS             complement(1308..2762)
                     /locus_tag="PKM23_12145"
                     /inference="COORDINATES: protein motif:HMM:NF005097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HNY92265.1"
                     /translation="MPSVILLLILPLFGAVLCALLPARHADKCAVGATGGMLLSLLWL
                     LLARPDGNWQSLPLTWRWLQGSFREGLFGFLLDPLALLLLLVVVVLGFLVVLYATAYL
                     GAGNREHADVTGKSRHHAWLLLFITAMIGVALSPNLLQLYLFWEMTTLCSWALISHYR
                     NPESLRAGFKALLMTFSGGLFFALGLVLLYVHTGSFAFDAHSDLAPRMRLWVFLSFAV
                     AAWAKSAQFPFFTWLPDAMAAPTTVSMYLHAAAMVKAGVFLMVRLALANPGLPWTAGL
                     VVAVLAVVTMLVALWLFFFQDDLKKLLAYSTIAHLSYVFLGVGVAIMGSRLGAYAGAM
                     HIMNHSVGKGLLFLSVGVLSYTAGTRRISELAGVARHSPLAAIAFMVGMLAILGVPPF
                     SGFWSKFYLMVAALKLGGAAGWLLLIPFALEIAVAFAWFLHIGHKVFFGPASGHAAKA
                     GALPGVMAAALIILMLLTLLAPWVAMQLMGAMGL"
     gene            complement(2821..3342)
                     /locus_tag="PKM23_12150"
     CDS             complement(2821..3342)
                     /locus_tag="PKM23_12150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNY92266.1"
                     /translation="MSGEINRGDVEEPRTGSCPLRPISGRVVVLGIGNPLCGDDDLGC
                     ETARLLQAGGFDHALVCEEVPENFTAEVKALQPREIILVDAVDFFAAPGDIISLRREQ
                     LRSDRFNTHKPALGLLMHYLEAETGAEVTLIGIQPKNRALHAPISPEVKESITHLAML
                     LAGLTLPASPIPV"
     gene            complement(3339..4178)
                     /locus_tag="PKM23_12155"
     CDS             complement(3339..4178)
                     /locus_tag="PKM23_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNY92267.1"
                     /translation="MDSEPMENIYLPDLAVIDRIVDETHDIKTFTLQFASRDLRKNFR
                     FKSGQFVEASIFGAGEAPFGFASNPNHPEHFDITVRAAGVVTRAMHALKPGDYMGIRG
                     PLGNHFPLEEVKGQDILIIAGGIGLPPLKSLIDPMLDCRGDFGQFIILYGARTPADRV
                     YKPLLQEWSERGDITLLQTVDTPDAAWSGPVGVVTTLFEKIKLAVDRTVAFTCGPPVM
                     IRFVIQELLAMGFAEERIISTLERYMKCGVGKCGHCAIGHKYICVDGPVFSWRQIKRL
                     PER"
     gene            complement(4168..>4598)
                     /locus_tag="PKM23_12160"
     CDS             complement(4168..>4598)
                     /locus_tag="PKM23_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNY92268.1"
                     /translation="QMVPTAYLAKACIQITNNRVREELWEEMGSECFSCGGCTHVCPC
                     CTCFDVVDCPEGAKSGTRVRCWDSCQYSGFTREASGHNPRARAKERVKRRFYHKLSYA
                     YIQQDGHPGCVGCGRCITVCEAFGLLDISAVVKRLRRDGQ"
BASE COUNT         1033 a         1339 c         1518 g          708 t
ORIGIN      
        1 cgcgcagggc cgcctgatag atcagccatt tactggcaaa gccgctgaaa ggcggtatgc
       61 cggcgatcga gagcgaggcg atgatggctg tcgccgccgt gaccggcagc accctcgcca
      121 ggcccccgac cttgttgata tcccgcgtgc ccgtcttgta gaaaaccgca ccggcattga
      181 aaaaaagaca tgatttgtag ctgacatgat tgatcaggtg aaaaatgccc gctcccagcc
      241 cgatcatacc gatgacgggc tgcgtccgca ggaaatagag cccggtgccg atggcgagaa
      301 acatatagcc catctggccg atgacgtgaa aggagagcaa ccgcttggca tcctcctgca
      361 ccagggcggt catggttccg accaggatgg aacccgcgcc gaaaagggcg atgatgaggc
      421 cccagatctg ggtgtggctg gagaggggca gcaaaccgca aaagacgcgg acgaggccgt
      481 aaatgcccag cttggtcatc gagccggcga aagcggcgct ggccggggca ggagccgctg
      541 gataggcatc cgggagccag tcgcccatgg gcaggatgcc cgccttggtg gcgaatccga
      601 ggaagaagca gcccaggacg aaatgaagca gtcccggctg gctctgctgc atttccgcca
      661 tcgccatttt gagaaaggag aaagaaaaag agccggaaag acgatagagg agaatgccgc
      721 caatgaagag aaatattgtg gcgatatggg tgatgagaaa atacttgtac cccgcccgca
      781 gccgtacgcg ctcttcttgt ctgaaaatga ccatgagata ggatgtgaga gtcatgatct
      841 cccaaaagac gaagaagaaa aagagatcag ccacgacgac cacattgagc accgccgcga
      901 aaaagaagag caggatgctg taaaaataac gcaggctgcc cgcgcgataa tgggcgatct
      961 gcatgaatct ttgggcatag agggtgacca gaaggctgac gacagcgatg atggcgagga
     1021 agagggcgct gagatagtcg atctggtaga tcagggccgc acccagtccg gggacctgca
     1081 gaatcggcag cccgctcgag accggaccct cccggaatac cccgaggacg gcggtcagga
     1141 tgaagagggt gccgaacgca gccgccccga aggccaccca gccgaccgcc ttgcgttttc
     1201 cgcccacaag cagggcgagg agcgcaccag ccagataaca tccctgcgcg gagaagaatg
     1261 cattcatctt acagatcctc gcgttggatt acggcagagc cttcctttta caagcccatc
     1321 gcccccatca gctgcatggc aacccagggg gccagcaggg tgaggagcat caggatgatc
     1381 agggcagccg ccataacgcc gggaagtgcg cccgctttgg cggcatgacc tgaggcaggt
     1441 ccgaaaaaga ccttgtggcc gatatgcaga aaccaggcaa aggccacggc aatttcaagg
     1501 gcaaagggga tcagcagcag ccagccggcg gccccgccca gcttgagtgc tgctaccatg
     1561 agatagaact tgctccagaa acccgaaaag ggcggcacgc ccaggatcgc gagcatcccg
     1621 accatgaagg cgatggcggc caatggggag tggcgcgcca ccccggccag ctcagagatc
     1681 cggcgcgttc cagcggtgta gctgagcact ccgacgctca gaaaaagcag tcccttgccg
     1741 acgctgtgat tcatgatgtg catcgcccct gcataggcgc ccagcctgct gcccatgatc
     1801 gccaccccta cgccgagaaa aacgtaggag agatgggcga tggtcgaata ggcgagcagc
     1861 ttcttgaggt catcctgaaa aaagaagagc cagagcgcca ccagcatggt caccaccgcc
     1921 aggacggcga cgacgagtcc cgccgtccag ggcaggccgg gattagccag ggccaggcgc
     1981 accatgagaa acacccccgc cttgaccatg gctgcggcgt gcagatacat gctcaccgtg
     2041 gtcggagcgg ccatggcatc cggcagccag gtgaaaaagg ggaattgggc cgatttggcc
     2101 caggcggcga cggcaaacga gagaaagacc cacagacgca tgcgcggggc cagatcggaa
     2161 tgggcatcga aggcgaagct gccggtgtgg acatagagaa ggaccagccc gagggcgaaa
     2221 aagagaccgc ccgagaaggt catcagcagg gccttgaaac cagcccgcag cgattccgga
     2281 ttgcgataat gcgaaatcag cgcccagctg cagagggtgg tcatctccca gaagagatag
     2341 agctgcagca gattgggaga gagggcgacg ccgatcatcg ctgtgataaa gagcagcagc
     2401 caggcgtggt ggcgggattt gcccgtgacg tcggcgtgct cccggttgcc cgcgcccagg
     2461 taggcggtgg catagagcac gaccagaaaa ccgaggacga ccaccaccag cagcaggagc
     2521 agggcaagtg ggtcaaggag aaagccaaag agtccttcgc ggaaagagcc ctgcagccat
     2581 cgccaggtga ggggcaggct ctgccagttc ccatccggtc tggccagcag cagccagagg
     2641 aggctgagca gcatacctcc cgtcgcgccg accgcgcatt tgtcggcatg gcgcgcgggc
     2701 aggagcgcgc aaagcacggc tccgaagaga ggcaggatca acaagagaat cacgctgggc
     2761 atcgtgcttt tccttttagc tgataagccg gccccctgcg cacagggggc cggccgcgca
     2821 tcataccggt atcggactgg ccggaagggt caggccggcg aggagcatgg ccagatgggt
     2881 gatgctctcc ttcacctccg ggctgatcgg ggcgtgcagg gcccggtttt tcggctggat
     2941 gccgatgagc gtcacttccg cgccggtttc agcctcgaga tagtgcatga gcaggccgag
     3001 ggccggcttg tgggtgttga aacggtcgct gcgcagctgc tcccggcgca gggagatgat
     3061 atccccaggc gccgcgaaga aatcgacggc atcgacaagg atgatctcgc gcggctgcag
     3121 ggccttgacc tccgcggtaa aattctcggg cacctcctcg cagaccaggg catggtcgaa
     3181 gcctccggcc tgcaagaggc gggcggtttc gcagccgagg tcgtcgtcac cgcaaagggg
     3241 attgccgatg ccaaggacaa ccactctccc agagatcgga cgcaggggac aactcccggt
     3301 gcgaggctct tccacatccc ccctgttgat ctccccgctc atcgctcggg cagccgtttg
     3361 atctggcgcc aggagaagac cggcccgtcc acgcagatat acttgtggcc gatggcgcag
     3421 tggccgcatt tgcccacccc gcacttcatg tagcgctcga gggtggagat gatgcgttcc
     3481 tcggcgaagc ccatggccag cagctcctga atgacgaaac ggatcatcac gggcggaccg
     3541 caggtgaagg ccaccgtgcg gtcaaccgcc agcttgatct tctcgaagag ggtggtcacc
     3601 acccccaccg gaccgctcca ggcggcatca ggggtgtcca cggtctggag cagggtgatg
     3661 tcgccgcgtt cactccactc ctgcagcagg ggtttgtaca cccggtcggc cggcgtgcgc
     3721 gcgccgtaga ggatgatgaa ctggccgaaa tcgccccggc agtcgagcat cgggtcgatc
     3781 agggatttga ggggcggaag cccgatgccg ccggcgatga tgaggatatc ctggcccttg
     3841 acctcctcga gggggaaatg gttgccaagg gggccgcgga tgcccatgta atcgccaggc
     3901 ttgagggcgt gcatggcccg ggtcaccacc cccgcagccc gaaccgtgat atcgaaatgt
     3961 tcgggatggt tgggattgga ggcgaagccg aagggggcct cgccggcgcc gaagatggag
     4021 gcctcgacga actgaccgga tttgaagcgg aaattcttgc gcagatcgcg cgaagcgaat
     4081 tgcagggtga aggtcttgat gtcatgggtc tcatcgacga tgcggtcgat gacggcgagg
     4141 tcgggcagat agatgttttc cataggctca ctgtccatcc cggcgcagcc gcttgaccac
     4201 cgcgctgata tcgaggagcc cgaaggcctc gcagacggtg atgcagcggc cgcagccgac
     4261 gcaaccaggg tgaccatcct gctggatata ggcgtagctc agtttgtgat agaaccggcg
     4321 tttgacccgt tccttggcgc gggcgcgcgg attatgcccg gaggcctcgc gggtaaagcc
     4381 ggagtactgg caggaatccc agcagcggac gcgggtcccg ctttttgctc cctcagggca
     4441 gtcgacgaca tcgaagcagg tgcagcaggg acagacgtgg gtgcaccccc cgcagctgaa
     4501 gcattcggag cccatctcct cccagagctc ctcgcgcacg cggttgttgg tgatctggat
     4561 gcaggccttg gcgagatagg cggtcgggac catctgcg
//
LOCUS       DAOPWU010000281         4597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_18859_length_4597_cov_3.738441, whole genome shotgun sequence.
ACCESSION   DAOPWU010000281 DAOPWU010000000
VERSION     DAOPWU010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18859_length_4597_cov_3.738441"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKM23_12165"
     CDS             complement(<1..355)
                     /locus_tag="PKM23_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY92269.1"
                     /translation="MLALKLAIRNLLGAGLRTWLTAGVLSIAFVVMVFYQGLLDGWNL
                     HARTTTREWEIGGGQYWHPAYDRFDPFSLQEAHAPLSHQALELAAAGRIVPELITQAT
                     AYPDGRMVNLLIRGIP"
     gene            complement(342..1121)
                     /locus_tag="PKM23_12170"
     CDS             complement(342..1121)
                     /locus_tag="PKM23_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY92270.1"
                     /translation="MENQKLIDIRNLVKRFPIGDGSFTALRGITLAFAEGEFAGLVGP
                     SGSGKTTLLNIIGSLDTPSEGSVTVLGREVTHLSHREAAALRNHHIGFIFQTYNLLPV
                     FTVYDNVEFPLLLQKGGAAGRKKAVMKALEWVGLADKAQSRPAQLSGGECQRVSIARA
                     MVKRPQLVLADEPSANLDADNSHHILRTMKRLNRELGTTFIFATHDEKVMHYLDRIIG
                     LEDGRVARDERMESAAEDDHPPASGEIGGSELPGEEDFARP"
     gene            complement(1133..2221)
                     /locus_tag="PKM23_12175"
     CDS             complement(1133..2221)
                     /locus_tag="PKM23_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92271.1"
                     /translation="MGQASLWGLYSSGARYPLGLGGRCLPQLNGQRTFSGRTVDLEAS
                     LHLRAGLAADPFDAAESDGDIKAYRLWVRFSAPQFEIRLGLQKINFGSALLLRPLMWF
                     DAVDPRDPLQLTDGAWGLLGRYYFLNNATLWLWLLRPADQPRSWELTPSHAGFPEWGG
                     RIQYPLPGGEIGLSGHLRTTDGRALAGALLPDGAARTQQEGLVEIRESRIGLDAKWDW
                     LAGWWFEGAWIHSHRSLGPYTDETLFTIGADYTFPVGNGLHLIGEHLWLATGAKGPGL
                     AGARGLAGLSLSYPLGLLDQLSAILYVDRGEEALYRFASWRRQYDHLAVNLMAFWNPE
                     SQLLPGSSNDGGAAFAGRGGQIIFVYNH"
     gene            2613..2954
                     /locus_tag="PKM23_12180"
     CDS             2613..2954
                     /locus_tag="PKM23_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92272.1"
                     /translation="MKDTTKAVMRTFFFLGGAYLFYTAFMFKPEPNEKWFLAFCGFML
                     LLSSCRATWAGIIMIVLGMSFCGLEYYNLSQPGIGINPDLGGYIWGLISMIVGGVFFT
                     FAPSKKKKDDQ"
     gene            complement(3002..>4597)
                     /locus_tag="PKM23_12185"
     CDS             complement(3002..>4597)
                     /locus_tag="PKM23_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNY92273.1"
                     /translation="FQEMISIRSERISHFSIIMRLWQAIHDRETPGEEPLSDSFYADL
                     EHILAGMSGKSAVYASGELPEFMHMSGNEAAVARSNELDTLASHTLAWIGRYAHGLQP
                     EVIERRRENRTRILARLGGSEEDWGDYRWHLRHLLRDSATIGSLIELTDEERGAVDRA
                     RASRLPFGVTPYYASLMDLEAHRRDDHAVRAQVLPPPFYVDTMREHDNDRAEYFDFMR
                     ETDTSPVDLITRRYPHIAIFKPYNTCSQICVYCQRNWEINDVLFPEALALPEKIDRAI
                     GWLSDHPAITEVLVTGGDPLVMPDHVLAGILDRLAAISHIERIRIGSRIVVALPQRMT
                     EALADLLARHHLPGRREVALITHFEHPYEVTPEAMEAVQKVRRRGISVYNQAVFTMEN
                     SRRFELVALRRQLRLIGVDAYYTFCAKGKDETRDYRVPIARLQQEVKEEARLMPGLVR
                     TDEPVYNVPRLGKNYLRAEQNHALVTILPDGSRLYEFHPWEKNLTLAPTYLHKDIPIL
                     DYLRELERRGEEMEEYRSIWYYF"
BASE COUNT          846 a         1317 c         1550 g          884 t
ORIGIN      
        1 gggggatgcc gcgaatgagc agattgacca tgcggccgtc gggataagcc gtggcctggg
       61 tgatcaactc cgggacgatg cgaccggcgg cggccagctc cagggcctgg tggctgaggg
      121 gggcatgggc ctcctgcagt gaaaagggat cgaaacggtc gtaggcggga tgccagtact
      181 gcccaccgcc gatctcccac tccctggtgg tggtgcgcgc atggagattc cagccgtcga
      241 gcagtccctg gtaaaagacc atcaccacaa aggcgatgga gaggactccg gcggtcagcc
      301 aggtgcgcag gccggcgccg agaagatttc tgatggccag tttaagggcg agcaaaatct
      361 tcctccccgg ggagttcaga ccccccgatt tcgccggagg cgggcgggtg gtcatcctcc
      421 gctgcgctct ccatccgttc gtcgcgggcc acccggccgt cctcgaggcc gatgatgcgg
      481 tcgaggtaat gcatcacctt ttcgtcgtgg gtggcaaaga tgaaggtcgt gcccagctcg
      541 cggttgagcc tcttcatcgt ccgcaggatg tgatgtgagt tgtcggcatc caggttggcg
      601 gagggctcgt cggcgagaac gagttggggc cgtttgacca tggcgcgggc gatggagacg
      661 cgctggcact ccccgccgga gagctgggcg ggtctggatt gggccttgtc cgccagtccg
      721 acccactcca gggccttcat taccgccttt ttacggccgg ccgcgccccc cttctgcagc
      781 aggaggggga actcgacgtt gtcgtaaacg gtaaaaaccg gcagcaggtt gtaggtctgg
      841 aagatgaagc cgatgtggtg gttgcgcagg gcggccgcct cccggtggct gaggtgggtg
      901 acctcacggc ccagcacggt cacggaacct tcgctggggg tgtcgagcga gccgatgatg
      961 ttgagcaggg tggtcttgcc ggagccgctc ggtccgacca ggccggcgaa ctcgccctct
     1021 gcgaaggcca gggtgatgcc ccgcagggcg gtgaagctgc catcgccgat gggaaagcgt
     1081 ttgaccaggt tgcggatgtc gatgagtttt tggttttcca tagtccgctt cattaatggt
     1141 tatagacgaa gatgatttgt ccccccctgc cggcaaaggc ggcgccgccg tcattggatg
     1201 acccgggcag gagctgactt tccggattcc agaaggccat cagattgacg gccagatggt
     1261 cgtactgccg ccgccagctg gcgaagcggt agagcgcctc ctccccccgg tccacataga
     1321 ggattgccga gagctgatcg aggaggccga gcggatagga gagcgagaga ccggccaggc
     1381 cgcgggcgcc ggcgaggccg ggacccttcg cgccggtggc gagccagaga tgttcaccga
     1441 tcaggtgcag accgttgccg acggggaagg tataatcagc cccgatggtg aacagggttt
     1501 cgtcggtgta ggggccgagg gagcggtggc tgtggatcca ggcgccctcg aaccaccatc
     1561 ccgccagcca gtcccatttc gcgtccaggc cgatgcggct ttcgcggatc tccacaagcc
     1621 cttcttgctg tgttcgggcc gcaccgtcgg ggaggagtgc gccggccagg gcgcggccgt
     1681 cggtggtgcg cagatgaccg gaaaggccga tctccccgcc gggcaggggg tactgaatcc
     1741 gtccgcccca ttcgggaaaa ccggcatggg agggggtcag ctcccagctg cggggctgat
     1801 ctgctgggcg gagcagccag agccaaagtg tggcgttgtt gagaaaatag tagcgtccga
     1861 gcagtcccca ggcgccgtcg gtgagctgca ggggatcgcg cggatcaacc gcatcgaacc
     1921 acatcagggg gcgcagcagg agcgccgagc cgaagttgat cttttgcagg ccgaggcgga
     1981 tttcgaactg gggcgcagaa aagcgcaccc agaggcgata ggccttgatg tcgccatcgc
     2041 tttcggcagc atcaaaggga tcggcggcga gaccggcccg aagatgaagc gaggcctcca
     2101 ggtcaaccgt cctgccgcta aaggtgcgct ggccgttgag ctgggggagg cagcggccgc
     2161 ccagtccaag gggatagcgg gcgccgctgc tgtacagtcc ccacagggag gcttgaccca
     2221 caacacggag tccgtcctcg gcggggagaa gaggacagag aggaagcaat attattgtaa
     2281 aaatcaaata tttatatttc attatgcgcc gtttcagagc ggaagaggcc gcatgcagcg
     2341 ggcggaaatt atccttgccc gcggttgatg aggcatgtat attgaagata aggcatttga
     2401 ttctctaatg caatgaggat ttgtggcctg ccggcgccgg ctggccggga aacaaaacgg
     2461 cgagacctcc atggcagccc ggtccgcacc tctgactggc cgcctgctgc agcgggggga
     2521 gccggtgctt caaggcgtgg ttgaaaattc gggcacacgc gtccgccaac cgtttacgtc
     2581 gaaaaggtcc gatagcgctg gaggagggga ttatgaaaga tactaccaag gcggttatgc
     2641 gcactttttt ctttcttgga ggcgcttatc ttttctacac agcttttatg ttcaaacctg
     2701 agccaaatga aaaatggttc cttgcttttt gtggtttcat gctgcttctt tcatcttgtc
     2761 gtgccacttg ggccgggatt atcatgatag tacttggaat gagcttttgc ggcctggaat
     2821 actacaatct ttcccagccg gggatcggca tcaatcccga tctcgggggc tatatctggg
     2881 gtttgatctc tatgatcgtc ggtggcgttt tttttacatt cgcaccctcc aaaaagaaaa
     2941 aagacgatca gtaggggcct gaggccgcgg aggaggctcc gcggtgcccc accctccctt
     3001 cctaaaaata gtaccagatg ctgcggtact cctccatctc ttcccctctc cgctccagct
     3061 cccgcaaata atcgagaata gggatatcct tgtgcagata ggtcggtgcc agggtcagat
     3121 ttttctccca gggatgaaat tcataaagcc ggctgccgtc cggcaggatg gtcaccaggg
     3181 cgtggttctg ttctgcgcgc aggtaattct tgcccaggcg cggcacgttg tagaccggct
     3241 cgtcggtgcg caccagcccg ggcatgagcc tggcctcctc cttgacctcc tgctggagcc
     3301 gcgcgatcgg gacgcggtag tcgcgggtct cgtccttgcc cttggcgcaa aaggtgtaat
     3361 aggcgtctac cccgatcagg cgcagctggc ggcgcagcgc caccagctcg aagcggcggc
     3421 tgttctccat ggtgaagacc gcctggttgt agaccgatat tccccgccgc cgcacctttt
     3481 gcaccgcctc catcgcctcg ggggtcacct cgtagggatg ttcgaaatgg gtgatcaggg
     3541 ccacctcacg gcggccggga aggtgatggc gcgccagcag gtcggccagg gcttcggtca
     3601 tgcgctgcgg cagggcgacg acgatgcgcg aaccgatgcg gatgcgctcg atgtgactga
     3661 tggcggcgag gcggtcgagg atgccggcca gcacatggtc gggcatgacc agtgggtcgc
     3721 ccccggtgac cagaacctcg gtgatggcgg ggtgatcgct cagccagcca atcgcccggt
     3781 cgatcttttc cggcagggcg agggcctccg ggaagaggac gtcgttgatc tcccagttgc
     3841 gctggcagta gacgcagatc tggctgcagg tgttgtaggg cttgaagata gcgatatggg
     3901 gatagcgccg cgtgatcaga tcgaccgggg aggtgtcggt ctcacgcata aaatcgaaat
     3961 attcggcgcg gtcgttgtcg tgctcgcgca tggtatcgac atagaagggt gggggaagca
     4021 cctgggcgcg gacggcatgg tcatcccggc ggtgcgcctc gaggtccatg agggaggcat
     4081 agtagggggt caccccgaag ggcaggcggg acgccctggc gcggtcgacc gcacccctct
     4141 cctcgtcggt cagttcgatg aggctgccga tggttgcgct gtcgcgcagg agatggcgca
     4201 ggtgccagcg gtaatccccc caatcctcct cactcccgcc caggcgggcg aggatgcgcg
     4261 tgcgattctc ccggcgtctt tcgatcacct ccggctgcag cccatgggca tagcggccga
     4321 tccaggcgag ggtatgggag gcgagcgtgt ccagctcgtt cgagcgggcg acggctgctt
     4381 cgttcccgct catgtgcatg aattccggca gctcaccgga ggcatagacc gcgcttttac
     4441 cggacatccc cgccaggatg tgctccagat cggcataaaa actatcgctc aggggctctt
     4501 ccccgggcgt ctcccggtcg tgaatggcct gccaaagccg catgatgatg ctgaaatggc
     4561 tgatccgctc gctgcgaatc gagatcatct cctgaaa
//
LOCUS       DAOPWU010000282         4565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19017_length_4565_cov_5.009313, whole genome shotgun sequence.
ACCESSION   DAOPWU010000282 DAOPWU010000000
VERSION     DAOPWU010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19017_length_4565_cov_5.009313"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PKM23_12190"
     CDS             <1..754
                     /locus_tag="PKM23_12190"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNY92274.1"
                     /translation="ALCGAEAAATPAALPEGGPALIFLAVPDDAIAAAAAQVAAAAKA
                     GPGTAVVHCSGSLPAAILAPLRPHTRRLASMHPLQSFAGRRQEWRRWRGIHIALEGEE
                     QTVVLLEEIARRLQSRPFRLTAGAKIDYHIACVLLSNCLVGLYDAADELLAGVELPAE
                     EKRAMTEPLLRNTIANVIASGPGAALTGPVSRGDTGTITAHLRALAAERPGLLELYRT
                     LSLRLVKLAEQEGRLTLLQSEQLRKLLNHPDE"
     gene            759..2000
                     /gene="dgt"
                     /locus_tag="PKM23_12195"
     CDS             759..2000
                     /gene="dgt"
                     /locus_tag="PKM23_12195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HNY92275.1"
                     /translation="MPLNLRKRSDLEKIEETILAPWAARSTRFAPTRLHAEPEHAYRT
                     AFQRDRDRIIHSRAFRRLKHKRQVFLTQSGDHYRTRITHTLEVSQLSRTMARTLGVNE
                     ELAEAIALGHDLGHTPFGHIGEVVLHDILSGTDTLDGTLPARDGGGFKHNYQSLRVVE
                     VLEKKYAWDGLNLTAAVREGILKHTRLRRQAINYPGFNLEGLHYELDNATTIEGQIVA
                     VCDEIAQRTHDLEDGIRAGFVSLERVRELPIIREIDRRAGLKLDDRFIYRNTLIRSLV
                     NFLVTDVMEATLGRIDTFMGLTGRSSHFDTLLVWFSDRVNPLQEELDRFIASEIIRIA
                     SEERSDDQAREVIRTLFRSYFLQPGLLPDYRLAPVLTGEGKELRKDSSFLRAIADHIA
                     GMTDNYAELENRRISEARHER"
     gene            1990..2835
                     /locus_tag="PKM23_12200"
     CDS             1990..2835
                     /locus_tag="PKM23_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY92276.1"
                     /translation="MSVDLQLQDICFRYTGIGASDRMILDHITLNLGGAPGCTAIVGP
                     SGSGKTTLIQHFTGLLRPSSGTVRVEGEDIWARGYDHTLLRRRIGLVFQFPEGQLFEE
                     SVARDVAFGPLNLGWSEERIDAAVEKALRQVRLDPVRFRDRSPFNLSEGEKRRVAIAG
                     VLAMEPEMVVLDEPTAGLDPRGLRSVEELIEGLIDQGTAVVLITHNMDFVNDLAGRVL
                     LMIGGRIAYDGPPAALFAEPTLLQAGSLEEPLFLRERAARGAHWPAHFRQAGSLRALL
                     RIVRK"
     gene            3075..4145
                     /locus_tag="PKM23_12205"
     CDS             3075..4145
                     /locus_tag="PKM23_12205"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY92277.1"
                     /translation="MNYLDELQLSDLQQEVEQQRPDLSFFETYYPKLSRSPEIQKIYS
                     LIDKIARSNATVLIQGETGTGKELIAGAIQAQSLRSREPYVTVNCAALPEHLLESELF
                     GHERGAFTGAVSSHVGKFEQADGGTLFLDEIGDMHPSTQAKILRVLQEQAFVRVGGQR
                     TINVDVRIIAATNRDLWEEMESGRFRTDLYYRLNVVTLHVPPLRERSEDILILADYFR
                     HKFAAEIKKQVGDFDDETRLLLANHSWPGNIRELRNLIERAVLVVNPNHTVTAKDLAM
                     TGKDYFAAGGRDRRRDEKRGPSLFTLNLEELERTAITIALQRSRWVQKEAAALLGISP
                     RALNYKITQYKISHASWRKNSA"
     gene            4241..>4565
                     /locus_tag="PKM23_12210"
     CDS             4241..>4565
                     /locus_tag="PKM23_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNY92278.1"
                     /translation="MNWKFLVFVTLLGAALAAAGDNSISITWSRNTEPDLLGYRLHFG
                     TRPGAYTASRDLGDVTTYTLTGLAADTTYYIALTALDHWGNESGYSNEVEAAPEGGGG
                     GGGNPA"
BASE COUNT          863 a         1589 c         1336 g          777 t
ORIGIN      
        1 agcgctgtgc ggagcagaag cagccgccac cccggccgcc ctgccggagg gtgggcccgc
       61 gctcattttc ctcgccgtcc ctgacgacgc catcgccgcg gccgcagctc aagtggctgc
      121 ggccgcgaaa gcgggacccg ggaccgcagt cgtccactgc tcgggctcgc tcccggccgc
      181 catcctggcg cccctgcggc ctcacacccg ccggctcgcc tccatgcacc ccctgcagag
      241 cttcgccggc cgcaggcagg agtggcggcg ctggcgcggc atccacatcg ccctcgaggg
      301 cgaggagcag accgtggtgc tgctcgagga gatcgcgcgg cggctgcaga gccgcccttt
      361 tcgcctcacc gccggggcca aaatagacta tcacatcgcc tgcgtcctgc tctccaactg
      421 cctggtcgga ctctatgatg cagccgacga actgctcgca ggggtggagc tgccggcgga
      481 agagaagcgg gcgatgaccg aacccctgct gcggaatacc atcgccaacg tcatcgcctc
      541 cggcccgggt gcggccctga ccggaccggt ctcgcgcggc gacaccggca ccatcactgc
      601 ccacctccgc gccctggcgg cggagcggcc cggcctcctc gaactctacc gcaccctctc
      661 cctccgcctg gtgaaattgg cggaacaaga gggccggctg acgctgttac aaagtgaaca
      721 gctccgcaaa ctgctcaatc atcccgacga atgacgccat gcccttaaac ctgcgcaaac
      781 gaagcgatct tgaaaagatc gaagagacca ttctcgcccc ctgggcggcc cggagcaccc
      841 gcttcgctcc gacccgcctc catgcggagc cggaacacgc ctatcgcacc gcctttcagc
      901 gtgaccgcga ccgcatcatt cactccaggg ccttccggcg cctcaagcac aagcgtcagg
      961 tcttcctcac ccagtcgggc gaccattacc gcacccgcat cacccacacc ctggaggtct
     1021 cacagctctc gcgcaccatg gcgcggaccc tgggggtcaa cgaggaactt gcggaggcga
     1081 tcgccctcgg ccatgatctt ggccacaccc ccttcggcca tatcggcgag gtggtcctcc
     1141 acgacattct cagcgggacc gacaccctcg atggaaccct tccggcccgg gatggcgggg
     1201 gattcaagca caactatcag agcctccgcg ttgtcgaggt ccttgaaaaa aaatatgcct
     1261 gggacggcct caacctcacc gccgctgtac gcgagggcat tctcaaacac acccgcctgc
     1321 gccgccaggc gatcaactat cccggtttca acctcgaagg gctgcattac gaactcgaca
     1381 atgccaccac catcgagggg cagatcgtcg cggtctgcga cgagatcgcc cagcgcaccc
     1441 acgatctcga ggacggtatc cgcgccggat tcgtcagcct cgaacgtgtc cgcgaactgc
     1501 cgatcattcg cgaaatcgac cgccgcgccg gcctgaagct ggacgatcgc ttcatctacc
     1561 gcaacactct catccgcagc ctggtcaatt ttctcgtcac cgacgtgatg gaagccaccc
     1621 tcggacgcat cgacactttt atgggcctca ccgggcggag cagccatttc gataccctgc
     1681 tggtctggtt cagcgaccgg gtcaatcccc tgcaggagga actcgaccgc ttcattgcca
     1741 gcgagatcat ccgcatcgcc tcggaggagc gcagcgatga ccaggcgcgc gaagtcatcc
     1801 gcaccctctt cagaagctac tttcttcagc ccggtctcct ccctgactat cgcctcgccc
     1861 cggtgctcac gggggagggc aaggaactgc gtaaagacag ttccttcctg cgcgccatcg
     1921 ccgaccatat cgccggcatg accgacaatt acgccgaact ggagaaccgg cgcatcagcg
     1981 aggcccgcca tgagcgttga cctccagctg caggatatct gcttccgcta caccggcatc
     2041 ggcgcctctg accgcatgat cctcgaccac atcaccctca atctcggcgg cgcgcccggc
     2101 tgcaccgcca tcgtcgggcc gagcggatcg ggcaagacca ccctcatcca gcatttcacc
     2161 gggctgctgc ggccgtcctc cggcacggtg cgggtggagg gagaggatat ctgggccagg
     2221 ggctatgacc acaccctgct gcgccgccgc atcggcctgg ttttccagtt ccccgaggga
     2281 cagcttttcg aggagagcgt cgcccgcgac gtcgccttcg gcccgctcaa cctgggctgg
     2341 agcgaagagc gtatagatgc cgcggtcgaa aaggcgctgc ggcaggtccg cctcgatccg
     2401 gtgcgcttcc gggatcgctc ccccttcaat ctcagcgagg gtgagaagcg gcgggtggcc
     2461 attgccgggg tgctcgccat ggaacccgag atggttgtgc tggatgagcc cacggccggg
     2521 ctcgatcccc gcggcctgcg cagcgtcgag gaactgatcg aggggctcat cgatcagggc
     2581 accgcggtgg tgctcatcac ccacaacatg gatttcgtca atgacctggc cgggcgggtc
     2641 ctgttgatga tcggcggccg catcgcttat gacggccccc cggcggcgct cttcgccgaa
     2701 ccgaccctgc tgcaggcggg gagtctggag gagcccctct ttctccggga gcgggcggcc
     2761 cgcggcgcgc actggccggc tcatttccgc caggccggca gcctgcgcgc cctgctcagg
     2821 attgtgcgaa aataatacaa ccggcttgaa tctgaacaac cgccgcggtg cccggcaccc
     2881 ctgcatcccg tatggccgcg ggtgagggcc tacgccgtta cggccgatcc gcctaagtct
     2941 ttgtcagaaa agtatttaat aaatcctaaa ttatgccgtg gttgtgacct gagtcaatcg
     3001 ccatggggag gtcatggcgt agtatttgca ggtgctgtcc aggagtgggg agaaatgtgc
     3061 cgggaggtag catcatgaat tatctggacg agcttcagtt gtcagacttg cagcaggaag
     3121 tagagcagca aagacccgac ctttccttct ttgagaccta ctaccccaag ctgagccgca
     3181 gtccggaaat ccagaaaatc tacagtctca tcgacaagat cgcccgcagc aacgccacgg
     3241 tgctgatcca gggtgagacc ggaacgggca aggagctgat tgcgggtgcg attcaggccc
     3301 agagcctgcg cagccgtgag ccctatgtga cggtgaactg cgccgccctg ccggagcatc
     3361 tgctcgagag cgagctcttc ggccatgaac gcggggcctt caccggagcg gtttcgagcc
     3421 atgtcggcaa gttcgaacag gccgacggcg gcaccctttt tctggatgaa atcggagaca
     3481 tgcatccctc cacccaggcg aagatcctgc gggttctaca ggaacaggcc tttgtccggg
     3541 tgggaggaca gcgcacgatc aatgttgatg tccgtatcat cgccgccacc aacagggatc
     3601 tgtgggagga gatggaatca ggaaggtttc gcaccgacct ctattaccgc ctcaatgtgg
     3661 tcactctgca cgtgcccccg ctgcgggaac gcagcgagga tatcctcatc ctggccgact
     3721 atttccgtca caaattcgca gcggagatca agaaacaggt gggcgatttc gacgatgaaa
     3781 cccggctgct tctggccaac cactcctggc cggggaatat ccgcgaactg cgcaacctga
     3841 tcgagcgggc cgtgctggtg gtcaatccca accacaccgt cacggccaag gatctggcga
     3901 tgaccggcaa ggattatttc gccgccggcg gacgcgaccg gcgccgcgac gagaagcgcg
     3961 gccccagcct cttcaccctc aaccttgagg agctggaacg cacggccatc actatcgctc
     4021 tgcagcgcag ccgctgggtg cagaaggagg ccgccgcact gctcggcatc tcgccgcgcg
     4081 ccctgaacta caaaataacc cagtacaaaa tatcgcacgc gagctggcgc aagaattcgg
     4141 cctgaccctg ccggctttta ccccgcccgc tcaacgccaa tccggcggag cccccgctgc
     4201 tccgctctca tccttgtatc ataacggcat gaccatccct atgaactgga aattccttgt
     4261 ttttgtcacc cttctgggcg ccgcgctggc cgctgcaggg gacaactcga tctcgatcac
     4321 ctggtcccgc aacaccgagc cggacctgct cggctaccgc ctccatttcg gcacccgccc
     4381 cggcgcctac acggcttccc gggatctagg cgacgtcacc acctacaccc tcaccggact
     4441 ggccgccgat accacctact atatcgccct caccgccctc gatcactggg gcaacgagag
     4501 cggctattcc aatgaggtcg aggccgcccc cgaaggcggc ggcggagggg gcggcaaccc
     4561 ggccg
//
LOCUS       DAOPWU010000283         4563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19026_length_4563_cov_4.479148, whole genome shotgun sequence.
ACCESSION   DAOPWU010000283 DAOPWU010000000
VERSION     DAOPWU010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19026_length_4563_cov_4.479148"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PKM23_12215"
     CDS             <1..890
                     /locus_tag="PKM23_12215"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNY92279.1"
                     /translation="MIRNRHLFQLKLKPEVVDATFSVEKFRKDVDKATAGMKMPKGVT
                     TQTEQIDAMAAEWIIPEQPVEGKVLLYIHGGGFISGSCTTHRMHVAKFAMGCQLKSLL
                     FDYRLAPEHPFPAAVEDCVAAYRWLSGQGYAAQDIIVGGESAGGTLTLSLLLALKEQG
                     IPLPKAAFAISPVTDLRCLADSFTYNAKNDIAPMGSWNVWTQLYIADNDPTLPLLSPQ
                     FGDYAGLPPLHICVGTHEIHLDDCVNVAQKAKAQGVEVTLSKWPRMVHAFPILSPLFP
                     EAKQALAEICEFVKNHTQQ"
     gene            954..>1316
                     /locus_tag="PKM23_12220"
     CDS             954..>1316
                     /locus_tag="PKM23_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNY92280.1"
                     /translation="MAKNDLKTRVNEASVTEFLNSVSDEQRRNDCLEILAVMKKITKE
                     EPKMWGASIVGFGSYHYKGKGGREGDWMLTGFSPRKQNLVLYLMGGFDDHAELLSKLG
                     KFTTGVGCLYIKKLEDVDR"
     assembly_gap    1317..1416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1417..2363)
                     /locus_tag="PKM23_12225"
     CDS             complement(<1417..2363)
                     /locus_tag="PKM23_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HNY92281.1"
                     /translation="MNNLKIATAQFEHKSGDKRCNLAVIASLAAKAADEGSQVIAFHE
                     CSITGYTFARRLSKGQMLELAEFIPDGESIQTLAGIAAKNDIVVLAGLFEKDEHDSLF
                     KAYVCVDKNGLVARYRKLHPFINPWLTPGDRYCVFEIHGWKCGILICYDNNIIENVRA
                     TKLLGAEIIFMPHVTMCTPSTRPGAGFVDPKLWENRENDPTSLRLEFDGMKGRAWLMK
                     WLPARAYDNAVYAVFANPIGMDDDQLKNGCSMIIDPFGDVIAECRSFDDSIATAVVTR
                     EKLTQAGGFRYLQARRPELYREIIGQSHNSEQKVIWLEG"
     gene            complement(2392..2952)
                     /locus_tag="PKM23_12230"
     CDS             complement(2392..2952)
                     /locus_tag="PKM23_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNY92282.1"
                     /translation="MDKEIITAVALGIGLSASTGFRVFVPLLIAGAAARFGILPMNES
                     FGWLSSNAGLGSLGVATVVEIAAYYIPFVDNLLDHISTPLSIGAGTLISASVLPIDNE
                     IMKWLSSLVLGGGSAALVQGATAFLRLGSSGTTGGAANFLLASGENIAAIVTPIFTIL
                     LPLVMAILILLTFWIAYRLIVRRRNV"
     gene            complement(2996..3979)
                     /locus_tag="PKM23_12235"
     CDS             complement(2996..3979)
                     /locus_tag="PKM23_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNY92283.1"
                     /translation="MKVLFIGGTGNISTSVSKLAVARGIELFLLNRGERGIEIPGAKI
                     LRGDINDAGVTAALLRDHHWDVVVDWIAYVESEVQRDIDLFRGRTRQYIFISSASAYQ
                     KPLLHPVITESTPLRNPYWEYSRNKIACEELLNRRYREEGFPMTIVRPSHTYDTIIPA
                     LIGSGGEYTLIDRMVRGEKVIVHGDGTTVWTLTHAEDFALGFAGLMGHQQAIGHAFHI
                     TSDESLTWNQVFQTLGEAVGVEPRIVHIPSDFICSQEPSLTGNLLGDKAYSVLFDNSK
                     IKRFVPEYRAVIPFNQGIRRTLAWFAADPARQVVNPQTNAVMDRILQAYER"
     gene            complement(3984..>4563)
                     /locus_tag="PKM23_12240"
     CDS             complement(3984..>4563)
                     /locus_tag="PKM23_12240"
                     /inference="COORDINATES: protein motif:HMM:NF023367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HNY92284.1"
                     /translation="SKGSHPRTITRKPGAEEKQTQKLLALMQMCYVGAPMIYYGDEVG
                     MWGANDPCCRKPMVWADLSYQPERVLPSGDLRADPQTVEVDRDLFQTYQKLIALRKSR
                     PVLSSGGFRTVVVDEARQLYGFERFDDASRLLVVLNNSRDEQQTLVGCETAGWRDLLT
                     GEAFTVNGDRLVSLTVPPRWGRVLDPIPISGE"
BASE COUNT          948 a         1269 c         1326 g          920 t
ORIGIN      
        1 ggatgatcag aaacagacac ctgtttcaac tcaagctgaa gcctgaagtg gtcgacgcca
       61 ctttttccgt ggaaaaattc agaaaggatg tggacaaggc gacagccggc atgaagatgc
      121 ccaaaggtgt cacaactcaa acagagcaaa tagatgccat ggcggcagag tggatcattc
      181 ccgagcagcc ggttgagggc aaggtgctcc tttatatcca tggcggcggc tttatttccg
      241 gttcatgcac aacccaccgc atgcatgtcg ccaaattcgc gatgggatgt cagctcaagt
      301 cactgctgtt cgattaccgg ctcgccccgg aacatccctt cccggcagct gtcgaggatt
      361 gtgttgcggc ctacagatgg ctttcagggc agggatatgc cgctcaggac attattgtcg
      421 gcggcgaatc cgccggcggc acactgacgc tttcactcct gttggcatta aaggaacagg
      481 ggatcccttt gccgaaagcg gcctttgcca tttcgccggt caccgacctg aggtgcctgg
      541 ccgattcctt cacatataat gcaaagaatg atatcgcccc catgggctcc tggaatgtgt
      601 ggacacaact ttacatcgcc gataatgatc ccactctgcc cctcttgtcg ccccagtttg
      661 gggattatgc cgggctcccc ccgcttcaca tctgtgtcgg cacgcacgag attcatctcg
      721 acgattgcgt gaacgtggcc caaaaagcca aggcgcaggg agtggaggtg acattgagca
      781 aatggccgcg catggtccac gcctttccca tcttatcccc gctgtttccg gaggcaaaac
      841 aggcgctggc agagatttgc gaatttgtga aaaatcacac tcaacaatga gcacatgctg
      901 taatggatgt aaaaagatct gttattcgtt ttctatgcaa aaagggaggt tatatggcaa
      961 agaatgatct gaaaaccagg gtgaacgagg ccagtgtgac ggaattcctc aacagcgtca
     1021 gcgacgagca aaggcggaat gactgccttg agattcttgc cgtcatgaaa aaaatcacca
     1081 aagaggagcc gaagatgtgg ggggcgagca ttgtcggctt tggcagctat cattacaaag
     1141 gaaaaggcgg gcgcgagggc gactggatgc tgaccggttt ctcgccgcgc aagcaaaatc
     1201 tggtcctcta tctgatggga ggttttgacg accatgccga actgctcagc aaactcggca
     1261 agttcaccac cggggtggga tgtctctata tcaaaaagct ggaggatgtg gacaggnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntccc cctcgagcca gatcaccttt
     1441 tgttcagagt tgtggctctg gccgatgatc tcccgatata actcgggtcg tctcgcctgc
     1501 agataacgaa atccgcccgc ctgggtgagt ttctcccgcg taaccaccgc cgtcgcaatg
     1561 ctgtcgtcaa aagagcggca ctctgcgatc acatccccga agggatcgat gatcatggag
     1621 cagccgtttt taagctggtc gtcatccatg ccgatcgggt tggcaaagac ggcataaacc
     1681 gcattgtcgt aagctctcgc cggcagccat ttcatcagcc aggccctgcc tttcatgcca
     1741 tcaaattcaa gtcgcaggga ggtcggatcg ttttcgcggt tttcccacag tttgggatct
     1801 acgaatccgg cccccggcct ggtggagggt gtgcacatcg tcacgtgcgg catgaaaatg
     1861 atctccgccc ccaacagttt tgtcgcccgg acattctcga tgatattgtt gtcgtaacag
     1921 atgaggatgc cgcatttcca gccgtgaatc tcaaaaacac aatagcggtc tcccggggtc
     1981 agccagggat tgatgaaggg atgcagtttc ctgtacctgg ccaccagccc gtttttgtca
     2041 acgcagacat aggccttgaa caaagagtca tgctcatcct tctcgaacag gccggccagc
     2101 acgacgatgt cgtttttggc ggcaatcccg gcaagggtct gaatgctctc gccatcaggg
     2161 ataaattccg ccaactccag catctgcccc ttcgacagcc ttctggcaaa ggtgtagccg
     2221 gtgatggaac attcatgaaa ggcgatcacc tgcgagccct catccgctgc ctttgcggcc
     2281 agggaggcga tgaccgcgag gttgcagcgc ttgtcgccgc ttttatgctc aaactgcgct
     2341 gtggctatct tgaggttatt catatcgggc tcttgtcgat gagacatcat ttcagacgtt
     2401 tctgcgccgt acgatcagcc gatatgcgat ccagaaggtc agcaaaatga ggatggccat
     2461 gacaagcggc aggaggatag tgaagatcgg cgtcacgatg gcagcgatgt tctcccccga
     2521 ggcgagcaga aaattggccg cgcccccggt ggtgccgctt gatcccagcc gcagaaatgc
     2581 ggtggccccc tgcaccaggg cggccgagcc gccgccaagg acgagactgc tgagccattt
     2641 catgatctcg ttatcgatcg gcagcaccga ggcggagatc agggtgccgg cgccgatgga
     2701 gaggggcgtg gagatgtggt cgagcagatt gtcgacgaag gggatgtaat aggccgcgat
     2761 ttccacgacg gtggccacgc cgaggctgcc aagcccggca ttgcttgaga gccagccaaa
     2821 gctctcgttc atgggcagaa ttccgaaacg ggcggcggca ccggcaatga gcagggggac
     2881 gaaaacccgg aagccggtgc tggcgctcag accgatgccc agggccaccg cggtgatgat
     2941 ctctttatcc atttttaata ttcctgatgt gaatcgaatc gcaccggcag gcgcgctacc
     3001 tttcgtaagc ctgcagaata cggtccatca ccgcattggt ctggggattg accacctgcc
     3061 gcgccgggtc ggccgcaaac caggccaggg tccgccggat gccctggttg aaggggatca
     3121 ccgcccggta ttccggcacg aagcgtttga tcttgctgtt gtcaaagagg acgctgtagg
     3181 ccttgtcccc gagcaggttg ccggtcagcg agggctcctg gctgcagatg aaatccgagg
     3241 ggatatgcac gatgcgcggc tcgaccccga cggcctcgcc cagggtctgg aagacctggt
     3301 tccaggtcag gctctcgtcc gaggtgatgt gaaaggcgtg gccgatcgcc tgctgatgcc
     3361 ccatcagccc ggcgaatccc agcgcgaaat cctcggcatg ggtcagggtc cagacggtag
     3421 tgccgtcgcc atgcacgatc accttttccc cccgcaccat gcggtcgatg agggtgtact
     3481 cgccgccgct gccgatgagt gccgggatga tggtgtcgta ggtgtgcgag gggcggacga
     3541 tggtcatggg aaagccctcc tcgcggtagc ggcggttgag gagctcctcg caggcgatct
     3601 tgttgcgcga gtattcccag tagggattgc gcaggggcgt cgactcggtg atgaccggat
     3661 ggagcagggg cttctggtag gctgaggcgg aactgatgaa aatatactgc cgggttcgtc
     3721 cgcggaagag atcgatgtcg cgctgcacct cactctcgac gtaggcgatc cagtcgacaa
     3781 ccacatccca gtgatgatcg cgcagcagag ccgcggtcac accggcatca ttgatgtcgc
     3841 cccgcagtat cttcgcgcca ggaatctcga tcccgcgctc gccgcggttg agcagaaaaa
     3901 gctcaatgcc gcgggcgacg gccagcttgc tgaccgaggt gctgatgttg ccggtgccgc
     3961 cgatgaacag gaccttcatg atatcattct ccggaaatgg gtatcggatc gagcactctt
     4021 ccccagcgcg gcgggaccgt cagtgaaacc aggcggtccc cgttgaccgt aaaggcttcg
     4081 cccgtcagca aatcccgcca gcccgccgtt tcacacccca ccagggtctg ctgttcatcc
     4141 cggctgttgt tgagtacgac cagcagacgc gacgcatcgt caaagcgctc aaagccgtag
     4201 agctggcggg cctcgtcgac cacaaccgtg cggaagcccc cgctgctcaa caccggtcgg
     4261 gatttgcgca gggcaatcaa tttttgatag gtttggaaca agtcgcgatc gacctctact
     4321 gtctggggat cggcgcgcag gtcgccggag gggaggactc tctcgggctg gtaggacaga
     4381 tccgcccaga ccatcggttt gcggcagcag gggtcattgg ccccccacat ccccacctcg
     4441 tctccgtagt agatcatcgg ggcgccaaca tagcacatct gcatcagggc gagcagcttc
     4501 tgggtctgct tctcctccgc cccgggcttg cgcgtaatgg tgcgcggatg gctgcccttg
     4561 ctg
//
LOCUS       DAOPWU010000284         4559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19058_length_4559_cov_4.749778, whole genome shotgun sequence.
ACCESSION   DAOPWU010000284 DAOPWU010000000
VERSION     DAOPWU010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19058_length_4559_cov_4.749778"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKM23_12245"
     CDS             <1..964
                     /locus_tag="PKM23_12245"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HNY92285.1"
                     /translation="FDVSTAWQAMTKAVFLRDGEEAPPVRAGYHELLKYQYIPFEQDT
                     STAWWVWGPVSTTQEYCLADWTLAQVAQKLGKSDAAAELLLRSGWYKNLFDPRLQLVR
                     PRLKDGSWLEPFDPLATEGSGSWAGSGGPGFVEGNAWQYAWFAPHDVAGLIGLFGGPA
                     PFSRKLQECFENGQFTINNEPDLAYPWLFTWLPGEESRAGRITAGLMANQFSTAPDGL
                     PGNDDCGTISAWFVFAALGFYPACPAAPEYRLSLPLFAEAELKLDRRYWPGESFIIRS
                     RGGDDRIELNGIPLAGCGLRHDQITAGGELCFDAMQEDDASRDQ"
     gene            1123..2802
                     /locus_tag="PKM23_12250"
     CDS             1123..2802
                     /locus_tag="PKM23_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNY92286.1"
                     /translation="MNRRKFIGTAAAGGLGAAFGAGRLFSAPLVLTADRTGVSTAQGE
                     LIFKPHFVQKGIGPHLLDWAYTSDENWDATYSNITAGREGVQVSDLYGVKKFGIDVRW
                     NVEGFGYIYITADNGGEFYQLPAPGKSSELNLNFELAKSRVLRSRRRLKQLQEGGWKP
                     SAELLGLVAISESHYEEAVKKAGDAALCASHAQPALLYAMWAGEKMELERAEYAIARR
                     GFRQGFYLGCDARGYFQMDTEIFMERFCDAFNYATITHYLKSGYFEDFEPEEGNKQFA
                     LRTELLKVLRRRNITVEGRPLFWFYHSTTPDWLRRKSFDQVMKYVEEHTRAVVSHYGD
                     GMYAWEVVNEFHDWANEIEVTPEQAVEITRLACEVARDTNPGVHRLINNCCPYAEYVQ
                     LKKWGEREARHHQRTPHQFMRDLVSAGVDFTITGQQLYFPYRDLQDTILLIERLEQFG
                     RPVQLTEVGASSGPNRNSVMTDRLGFPEEPYIWHRPWDEELQADWLEGLYTLAYSKPW
                     IEAVNWYDFLDPFAFIKNGGLLRSPQGEKKAAYERLINLQNKWRKLPQHHE"
     gene            2821..4365
                     /locus_tag="PKM23_12255"
     CDS             2821..4365
                     /locus_tag="PKM23_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNY92287.1"
                     /translation="MAHGKLIFKPWLVQRGRGPHLDPFVYATDANGDTFHSDIRVTRE
                     GIEISDTEGEERFALNLRWNVEGYGYLFMSVDPGPEFYRLPASGQRTLNLNFELAASR
                     TRRNQQRLEKFIAAGFQPSRELEALLVLASEYLEDAGRLQADGERCARASQESLKYGL
                     HASDLLEIEKARFDISARSSRPPFLMGCDGRGFFQMDESLFLDLFSSLFNYATITHYL
                     IGDVHNFEPEEGKKQYAERERLLQALRRRGITVEGRPLLWLHKWVTPEWLIHKSYDGV
                     KKYLENHVRAVVGHYGDEISVWEVVNELHDWANELELNHDQTIELTRLACDVARDANP
                     KVRLLINNCCPFAQYVQMGKWCDKEARYPQRTPHQFVRQLIEAGVDFDIAGVQMYFTK
                     QLLADCVLMIERYQGLGKCVHLTEVGSPSAGMTMEFADQEKIPWSAQPYEWRRHWDEE
                     LQADWLEAVFTVANSKPWIEAANWYDFVDPYGYLKSGGLLRSPQGEKKAAYDRFLRLK
                     QQWQVQ"
BASE COUNT          954 a         1350 c         1336 g          919 t
ORIGIN      
        1 ttttgacgta tccaccgcct ggcaggcgat gaccaaagcg gtatttctcc gtgatgggga
       61 ggaggcgccg ccggtacgcg ccggttatca tgaactcctg aaatatcagt acattccctt
      121 cgaacaggat acctcgaccg cctggtgggt ctggggaccg gtatccacga cgcaggagta
      181 ctgcctggcc gactggaccc tggcgcaggt ggcccaaaaa ctgggcaaat ccgacgcagc
      241 cgctgaactg ctgctccgtt ccggttggta taaaaatctc tttgatcccc ggcttcagct
      301 ggtgcggccg cgcctgaagg atggctcatg gctggagccc ttcgatcccc tggccacgga
      361 gggttcgggc tcatgggcgg gctcaggcgg ccccggcttc gtcgaaggca acgcctggca
      421 gtacgcctgg ttcgcacccc acgacgtcgc agggctgatc ggactcttcg gcgggccggc
      481 tcccttttcg cgcaaactgc aggagtgctt tgagaacggc cagttcacca tcaacaacga
      541 acccgacctc gcctatccat ggctcttcac ctggctgccg ggagaggaga gccgcgccgg
      601 ccgcatcacc gctggcctca tggcgaacca attcagcact gccccggacg gcctgcccgg
      661 caatgacgac tgcggcacca tatcggcctg gttcgttttc gccgccctcg gcttttaccc
      721 ggcttgtccc gccgctccgg agtaccgact cagcctgccg ctctttgcag aggccgagct
      781 gaaactcgac cggcgctact ggccggggga atctttcatc atccgcagca ggggcgggga
      841 cgaccggatc gagctcaacg gcattccttt agccggttgt ggcctccgcc acgaccagat
      901 cacggcagga ggggaactct gcttcgatgc catgcaagag gacgatgcgt cacgtgatca
      961 gtagacccgc catcctgccg cttctcctgc tcagaggata acagctggtc ggatagtgat
     1021 tcgtgatcag acagactgga atggagagca gaccgaccct ggcgccagta gtctgaaata
     1081 ccaggggcat gcagttcagg caatgaatta aggagtgagt atatgaacag acgaaaattc
     1141 atcggaactg ctgctgcggg cggtctcggg gccgcattcg gcgctggcag gcttttttcc
     1201 gcacccctgg tgctgacggc ggatcgcacc ggcgtctcga ctgcgcaggg cgaactgatc
     1261 ttcaaacccc atttcgtgca gaaggggata gggccgcatc tcctcgactg ggcgtatacc
     1321 agcgatgaaa actgggatgc aacctactcg aatatcactg ccggcaggga gggcgtccaa
     1381 gtatcagacc tctatggcgt gaagaaattc ggcatcgatg tccgctggaa cgtggaagga
     1441 ttcggctata tctatatcac ggccgacaac ggcggtgagt tctaccagct gcctgctccc
     1501 gggaagagca gcgagttgaa ccttaatttc gagcttgcca aaagcagggt tctgcgcagc
     1561 cgcaggcggc tgaagcagct gcaggagggg ggatggaaac cttcggccga gctgctgggc
     1621 ctggtcgcta tttccgaatc gcactacgaa gaggccgtta aaaaagcagg cgatgccgct
     1681 ctctgcgctt cacacgccca gcccgccctg ctttatgcga tgtgggcggg tgaaaaaatg
     1741 gagctcgaga gggcggaata tgccatcgcc cggcgtggat tccgccaagg attttatctc
     1801 ggctgcgatg cccggggcta tttccagatg gatacggaaa ttttcatgga gcgcttttgc
     1861 gatgctttta attatgccac catcacccac tatcttaaaa gtggctactt tgaggatttc
     1921 gagcccgagg agggcaacaa gcagttcgcc ctccgcactg agctcctcaa ggtgctgcgc
     1981 cggcgcaata ttaccgtcga aggccgtccc cttttctggt tttaccactc cacgacacca
     2041 gactggctgc ggcgcaaatc cttcgatcag gtcatgaaat atgtcgagga gcacacccgg
     2101 gcggttgtca gccactacgg cgacgggatg tacgcctggg aagtggtcaa tgaatttcac
     2161 gactgggcga atgaaattga ggtcacacct gagcaggccg tcgagatcac ccggctcgcc
     2221 tgtgaagtgg ccagggatac caatcccggg gttcatcgcc tcatcaacaa ctgctgcccc
     2281 tatgcggagt atgtccagct gaaaaagtgg ggtgagcggg aggcccgcca tcaccagcgc
     2341 accccgcacc agttcatgcg tgatctcgtc agcgcggggg tcgatttcac catcaccggc
     2401 cagcagctct acttcccata tcgcgatctc caggatacca tcctgttgat tgaaaggctc
     2461 gagcagttcg gccggcccgt gcagctgacc gaggtcggcg cctcctcggg cccaaaccgt
     2521 aattcggtga tgaccgaccg gctcggtttc cccgaggagc cctacatctg gcaccgcccc
     2581 tgggatgaag aactgcaggc tgattggctc gaggggctct acaccctcgc ctacagcaag
     2641 ccctggatcg aggccgtaaa ctggtacgat tttctcgatc ccttcgcctt catcaagaat
     2701 ggaggactgc tgcgctcgcc ccagggcgag aaaaaggccg cctatgagcg gctgatcaat
     2761 ttgcaaaaca agtggcgcaa attgcctcaa caccacgaat agccataaag gatacaacag
     2821 atggcccacg gcaagctgat tttcaaaccc tggctcgtac aacgagggcg cggccctcat
     2881 ctcgatccct ttgtttatgc caccgatgcc aatggtgaca cctttcactc cgatatcagg
     2941 gtcacccgcg aggggatcga aatttcggac accgaggggg aggagcgctt tgccctcaat
     3001 ctgcgctgga atgtggaggg gtatggatac cttttcatgt cggtagaccc cggccctgaa
     3061 ttctaccgcc tgcctgcatc cggacaacgc accctgaatc tgaattttga actggccgcg
     3121 agcagaacaa ggcgcaatca gcagcgtctg gagaaattca tcgctgctgg ctttcagccc
     3181 tcccgtgaac tcgaggccct gctcgtgctc gcttctgaat atcttgaaga tgccgggcgg
     3241 ctgcaagccg atggagaacg atgcgcccgg gccagccagg aatcccttaa atacgggctc
     3301 cacgccagcg acctgctcga gatagaaaag gcccgttttg atatttccgc tcgttcgagc
     3361 aggccaccct tcctgatggg ctgcgacggc cgcggctttt tccagatgga tgagtctctt
     3421 tttctcgatc tcttttcctc gctgttcaat tatgccacca tcacccacta tctcatcggt
     3481 gatgttcata atttcgagcc ggaggagggg aaaaagcaat atgcggagcg cgaacggctc
     3541 ttgcaagccc tgcgccggcg cggcatcacc gtggaggggc ggcccctgct ctggctgcac
     3601 aagtgggtca cccccgagtg gctgatccat aagagctatg acggagtgaa gaaatacctg
     3661 gagaatcatg tgcgtgccgt ggtcggccat tacggggacg aaatctcggt ctgggaggtg
     3721 gtgaatgagc tgcatgactg ggccaacgaa ctggagttga accacgacca gaccatcgaa
     3781 ctcacacgcc tggcctgcga tgtcgcgcgc gatgccaatc cgaaggtgcg gctgctcatc
     3841 aacaactgct gcccctttgc ccagtatgta caaatgggca agtggtgcga caaggaggcc
     3901 agatatcccc agcgcacccc tcatcagttc gtcagacagc tcatcgaggc gggggtggat
     3961 tttgatatcg ccggagtgca gatgtacttc accaaacagc tgttggctga ctgcgtgctg
     4021 atgatcgagc gctatcaggg gctgggcaag tgcgtccatc tcaccgaggt cggatcgccg
     4081 tcggcaggaa tgactatgga attcgccgac caggagaaga tcccatggtc tgcccagccc
     4141 tatgaatggc gccgccactg ggacgaagaa ctgcaggccg actggctgga agcggtcttt
     4201 actgtcgcca acagcaagcc ctggatcgaa gccgccaact ggtacgattt tgtcgatccc
     4261 tacggttacc tcaagagcgg cggattgttg cgctctccgc agggggagaa aaaggcggct
     4321 tacgaccggt tcttgcggtt gaaacagcag tggcaggtgc agtgaatgat caacgagagg
     4381 aaatgatggc tccactatca agacgcgatt ttatcaaggc cggcgccctg actacggctg
     4441 ccgccctcac tgccgatttc ggcaccggcg cccctctttt tgccgcgagc gcgcaggaaa
     4501 tgcgcttcca gccctggcct catgcctgga tgccggattt cggccatgtc tatgccgcc
//
LOCUS       DAOPWU010000285         4557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19073_length_4557_cov_4.446912, whole genome shotgun sequence.
ACCESSION   DAOPWU010000285 DAOPWU010000000
VERSION     DAOPWU010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19073_length_4557_cov_4.446912"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /gene="dxs"
                     /locus_tag="PKM23_12260"
     CDS             complement(<1..1774)
                     /gene="dxs"
                     /locus_tag="PKM23_12260"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNY92288.1"
                     /translation="MDEQLQRINSPEDIKKLDIPQLTQLAREIRDYIIQTTSETGGHL
                     APSLGVVELTLALHHVLNTPQDKIVWDVGHQAYAHKIITGRREAFHTIRQDGGISGFP
                     KPAESPYDAFGVGHASTSISAAFGIACARDLAEEDFRVVAVIGDGALTGGLAYEGLNN
                     AGASGKDIIVIVNDNSMSISSNVGAMSKYLTNLISNPLYNKIKSDIWDFTGKFERMGP
                     RIRVAARRMEESLKGFIMPGVLFERLGFRYFGPIDGHNFAEMLHTMHEIKELRGPILL
                     HILTKKGRGYRPAEENAPVFHGLGKFDKVTGVPVKSGSVPSYTEVFGRAMCDLAPMQP
                     RMVGITAAMSLGTGLHLFGEKYPQRFFDVGIAEGHAVTFAAGMAAQGARPVVAVYSTF
                     LQRAYDHLIHDVALQKLPVVLAIDRAGIVGDDGPTHHGIFDIAYLRTVPDLVVMAPRD
                     EEELRHMLFTALNYDQGPVAIRYPRGAGEGVELTSGFDLLPIGRSETLDQGEEIALLA
                     VGHLARKASAAARFLREQEGIAVEVVNARFIKPLDTGMLDDLAARFKVIITLEEGTLA
                     GGFGSAVAEYLADHHTGKAALMRCG"
     gene            complement(1850..2071)
                     /gene="xseB"
                     /locus_tag="PKM23_12265"
     CDS             complement(1850..2071)
                     /gene="xseB"
                     /locus_tag="PKM23_12265"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNY92289.1"
                     /translation="MPPKKFEAAIARLEEIAALLEEGSAPLEESLRLYEESAELLAFC
                     IAQLEEAEKKINLLVKTSEGFQVEETDLD"
     gene            complement(2103..3356)
                     /gene="xseA"
                     /locus_tag="PKM23_12270"
     CDS             complement(2103..3356)
                     /gene="xseA"
                     /locus_tag="PKM23_12270"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNY92290.1"
                     /translation="MEMWNTAPASFSVAEFTRRIKTLLETELEPVWLHGEISNFVHHS
                     SGHMYFSLKDESAQISCVMWRSRNQGLILTPSDGMQVLCYGQVTLYEKRGNYQLDVMK
                     MIPAGVGQLQLALERLKARLHAEGLFDEEHKQPLPLFPRRIGIVTSPTGAAIRDLVQV
                     ARRRMPSIELVLRPALVQGEGAAEDIAAAIAELNEYGDVDVLIVGRGGGSLEDLWAFN
                     EEVVARAIYDSRIPVVSAVGHEIDFTITDFVADLRAPTPSAAAELVVPDAGELRSRLT
                     GTVKFLRHALQRRIAHERLRLAGFRSSYGLRRPIDLVRQRRQRLDELAHLCSISLLTR
                     LRRERLRAVNAAQRCRDLAPASVLERGYALCWREGDEAFLRSAEGLQPGETIMVQLAA
                     DRLASEIVGIHPGLTVVDDRKRPAK"
     gene            complement(3596..3874)
                     /locus_tag="PKM23_12275"
     CDS             complement(3596..3874)
                     /locus_tag="PKM23_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNY92291.1"
                     /translation="MTKAEIVDIVAEGTGLTKTETQAVIEGFLATVGYALRQGDRVDL
                     RGFGNFKVVDRDTRSTFNPVTKEPVVIPAHRAPLFKPSKELKKFINKK"
     gene            complement(4249..>4557)
                     /locus_tag="PKM23_12280"
     CDS             complement(4249..>4557)
                     /locus_tag="PKM23_12280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY92292.1"
                     /translation="CMALPSYAEVFPLSLLEGYAAGLPVVSTRVGGIPDFVQEGENGL
                     LVEPGDQEGLAAALTRLLTETPLRCRMAEANRRVARERYDIDICAQRVREIYQELLRQ
                     "
BASE COUNT          859 a         1402 c         1403 g          893 t
ORIGIN      
        1 ggccacagcg catcagtgct gcctttccgg tatggtgatc agcgagatac tcagccaccg
       61 cgctgccgaa tccgcccgcg agggtgccct cctccagggt gatgatgacc ttgaacctgg
      121 cggccagatc atcgagcatc ccggtatcaa gcggcttgat gaagcgggca ttgacgacct
      181 ccacagcgat cccctcctgc tcgcgcagga agcgggcggc ggcgctggcc ttgcgggcga
      241 gatggccgac ggcaagcagg gcgatctcct cgccctggtc gagggtttcg ctgcggccga
      301 tggggagaag atcaaagccg ctggtgagtt ccactccctc gccggctccc cggggatagc
      361 ggatggccac cgggccctgg tcgtaattca gggctgtgaa aagcatgtgg cgcagctcct
      421 cctcatcccg gggcgccatc accaccaggt cgggcaccgt gcgcaggtag gcgatgtcga
      481 agatgccgtg gtgggtgggt ccgtcatccc cgacgatgcc ggcgcggtcg atggcgagca
      541 ccaccggcag tttttgcagg gcgacatcgt ggatgagatg gtcataggcg cgctgcagaa
      601 atgtggaata gactgccact accggccggg cgccctgcgc agccatcccc gcggcaaagg
      661 tcacggcgtg cccctcggcg atcccgacgt cgaaaaagcg ctgcggatac ttctcaccga
      721 agaggtgcag tccggtgccc agggacatcg cggcggtgat gccgaccatc ctgggctgca
      781 tgggggcgag atcgcacatc gcgcggccga aaacctcggt atagctcggc acactgccgc
      841 tcttgactgg cacccctgtc accttgtcga atttgcccag gccgtgaaag accggtgcgt
      901 tctcctccgc aggacggtag cccctcccct tcttggtgag gatgtggagc aggatcggac
      961 cgcgcagctc cttgatctcg tgcatggtat ggagcatctc ggcgaaattg tgaccatcga
     1021 tcgggccgaa atagcggaag ccgagccgct cgaaaaggac gcccggcatg ataaaaccct
     1081 tgaggctctc ctccatccgg cgcgcggcga cacgaatgcg cggccccatg cgctcgaatt
     1141 tgccggtgaa atcccagatg tccgatttga tcttgttgta gaggggattg gagatgagat
     1201 tggtgagata cttggacatg gcgccgacgt tgctcgagat cgacatgctg ttgtcattga
     1261 cgatgacgat gatgtccttt cccgaagccc cggcgttgtt gagcccctca taggccaaac
     1321 ccccggtaag ggcgccgtcg ccgataacag cgaccacccg gaaatcctcc tccgccagat
     1381 cacgcgcaca ggcgatgcca aaggctgcag agatggaggt gctggcatgg ccgacaccga
     1441 aagcatcgta gggactctcg gccggcttgg gaaaaccgct gatcccgcca tcctggcgga
     1501 tggtgtgaaa ggcctcgcgc cggccggtga taatcttgtg ggcgtaggcc tgatggccga
     1561 catcccagac aatcttgtcc tgcggggtat tgaggacatg atgcagggcg agggtgagtt
     1621 cgaccacccc gagcgacggg gccagatggc ctccggtctc gctggtggtc tggataatat
     1681 aatcgcggat ctcgcgcgcg agctgggtca attggggaat atcgagcttc ttgatatcct
     1741 ctggcgaatt gatgcgttgc agctgttcat ccatatacta cctttgcagc atcctttgat
     1801 gacatcttta ccggttgggc cattggctat aacaacccgt aatagcaatc taatccagat
     1861 cggtctcctc gacctgaaaa ccctccgagg tcttgacgag cagattgatc ttcttctccg
     1921 cctcctccag ctgggcgatg caaaaggcca gcagttcagc gctctcttcg tagagccgca
     1981 gggactcctc cagcggggcg ctgccctctt ccaggagggc ggcgatctct tccagacgcg
     2041 caatggccgc ttcgaatttt ttggggggca tgcttcactc cttccgtcag gtggggatca
     2101 gatcattttg ccgggcgctt gcggtcgtcc actacggtca ggccgggatg gatgccgaca
     2161 atttcactgg ccagccggtc agccgcgagc tggaccatga tggtctcgcc cggctggagt
     2221 ccttccgcgc tgcgcagaaa ggcctcatcg ccctcgcgcc agcacagggc gtagcctcgc
     2281 tcgagcacgg aggcgggcgc caggtcgcgg cagcgctggg cggcattgac cgcgcgcagc
     2341 cgctcacggc gaaggcgggt gagcagtgag atcgagcaga gatgggcgag ctcgtccaga
     2401 cgctggcggc gctggcggac caggtcgatg ggccggcgca ggccgtagct ggagcggaag
     2461 cccgccagcc tcagccgttc atgggcgatc cggcgctgga gggcgtggcg gaggaacttg
     2521 acagtgcccg tcagccggct gcgcagctcg cccgcatccg ggaccaccag ctccgctgca
     2581 gccgaggggg tcggggcgcg caggtcggcg acgaaatcgg tgatggtgaa atcgatctca
     2641 tgacccaccg ccgataccac tggaatgcgc gagtcgtaga tggcccgggc gaccacctcc
     2701 tcgttgaagg cccagaggtc ctcgaggctg cctccccccc gtccgacgat gaggacatcc
     2761 acatcgccat attcgttcaa ctcagcgatc gccgcggcga tatcctccgc agccccttcg
     2821 ccctgcacca gggccggcct cagcaccagc tcgattgagg gcatgcgcct gcgggcgacc
     2881 tggaccagat cgcggatcgc cgctccggtc ggactggtga caatgccgat gcgccgggga
     2941 aagagcggca gcggctgctt gtgctcctcg tcgaaaagcc cctccgcgtg gaggcgggcc
     3001 ttgagcctct ccagggcaag ctgcagctgt ccgacccccg ccgggatcat cttcatcaca
     3061 tcgagctgat aattgccgcg cttctcgtag agcgtcacct ggccgtagca gagcacctgc
     3121 atgccatcgc tcggggtgag aatgaggccc tggttgcggc tgcgccacat cacacaggag
     3181 atctgggcgg actcatcctt gagcgaaaaa tacatgtggc ccgagctgtg gtggacgaaa
     3241 ttggagattt cgccgtgcag ccagaccggc tccagctcgg tttcgagcaa ggttttgatc
     3301 cggcgcgtga attccgcgac cgaaaagctg gcaggggctg tgttccacat ttccatggcg
     3361 tctatccggg tggttccggc cgtgcagatt gcgcccgccg gtgaaagtaa aagggccaag
     3421 atgcaactct cagccctata taaagattag cgtaatttct tcttgtgacg atctttgcgc
     3481 aatctttttt tacgtttatg cgtggcaatc ttggctcttt ttctcttttt tccgcttggc
     3541 aaatcgatca cctcctatcc ttgcgcagcc tcgatcggcc gcgtgttctt gcagatcatt
     3601 tcttattgat gaatttcttc aactctttcg agggtttgaa cagcggcgcg cgatgggcgg
     3661 ggatcaccac cggctccttc gtgaccgggt tgaatgtgga gcgcgtatcg cggtcgacga
     3721 ccttgaaatt gccgaagccg cgcaggtcaa cccgatcgcc ctgcctgaga gcatagccga
     3781 cggtggcgag aaatccttcg atcactgcct gggtttcggt cttggtcagc cctgtaccct
     3841 cagcgacgat atcgacaatc tctgctttgg tcataatggc atcctttcac acagacggga
     3901 tcagtgacag tggctgcaat catggctggc gcatgttcca caggaacggc ccgatgatga
     3961 agaacgcgag acctctccgc tttttccctt gctggtaaaa ccgcaggccg aaatgagacg
     4021 ctccacctcc ctgctcaggc agtttggaca actgggctgc tgcgctgcag agactgagga
     4081 gaagagctcc tcgaactggt ctccgcaatc ccggcattta aattcataaa gcggcatcgt
     4141 caggatggcc tcgtcggcac tatccgggga gatggattca tcataaagta taccaatttt
     4201 atgtcaaaaa agcaagataa aatctgtgaa acgcgccgcg ctgccggcct actgcctgag
     4261 cagctcctgg tagatctccc tcacccgctg cgcacagata tcgatgtcat agcgctcccg
     4321 tgccactctc cggttggcct ccgccatcct gcagcgcagg ggcgtctccg tcagcagccg
     4381 ggtcagcgcc gccgccagtc cctcctggtc ccccggctcg acgagcaggc cgttctcccc
     4441 ctcttgtaca aaatcaggga tcccccccac ccgggtcgag accaccggca gcccggcggc
     4501 atagccctcg agcagggaga gcggaaagac ctcggcgtag gagggcaggg ccatgca
//
LOCUS       DAOPWU010000286         4542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19163_length_4542_cov_4.533987, whole genome shotgun sequence.
ACCESSION   DAOPWU010000286 DAOPWU010000000
VERSION     DAOPWU010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19163_length_4542_cov_4.533987"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="PKM23_12285"
     CDS             <1..1004
                     /locus_tag="PKM23_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNY92293.1"
                     /translation="FRKYESLSIQYSRGCPFQCDFCNITAMLGRRPRTKRAAQIIAEL
                     DKIYRLGWRRNVFFVDDNFISNKRQLKEEILPALIAWRRGRAGCSYLTEASINLADDE
                     ELMGMMSAAGFNSVFVGIETPDEASLAECNKGQNVHRNLVECVQAMQRHGLQVMGGFI
                     VGFDNDTESIFDRQIEFIQKSGVVTAMVGMLQAIPGTPLFARLHAEGRITHEMTGDNA
                     DGTTNIIPAMGLDKLKTGYARLMAELYSPENFYARIRTCLRLYHPPKAPVRLHAEELY
                     AFLRSIWKLGTLSAERNHYWRLFFWTLFHRPRSLALAVTFTIYGFHFRTVNALNGIR"
     gene            1308..3893
                     /locus_tag="PKM23_12290"
     CDS             1308..3893
                     /locus_tag="PKM23_12290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNY92294.1"
                     /translation="MPETDLNNGGTGNMIAGVDFDNDGKTEIYLVNDNWNDTPTEVIP
                     RIYKLEKEGSEWVVVWKAVAPIEKQNTWPFLTHGDLDKDGKHEIYWGVVNNLSGTTGL
                     NPYRILVYESKGDGSDVMGVDDGVGGYLPNAKWTISAADNDNIRPIQACIADPDNDGV
                     DELLFADRAGRTGGVFCGIVSVDKIPDNGDGTEVWTMETSGLDFTLKAGTTQNKWDVV
                     AIGANVYSFCEVEVTKFTWDGFGWGYTKLSPLLGGAPVQSTQVVDLNGDGVKEMIVAV
                     YDWGNDAAKGIYLVQEDGDTLKNTELVNVGKWWPSGSRGLWGGDSGDIDGDGKLDFVY
                     GSRASTPNAGIFHLAYKGGDIADPASYTFNMIDSLYAEGGIWTVVKLANVDSDPQLEV
                     LYTSSTDAGAFPELGTKPIVVLDYVGGGAPVFDDLVVATEVKLNGEAPTGLLFKPGRI
                     LDNGRTIWFCGVDGTNKVTWVFRSVDGGQTFTHNATALVERAAQMDAWDANTAVIAQA
                     DGKIYRTTDGGVNWTEVYSYTISPIAPGWFDGLRVINGTSAIAFGDMEPDGNMHFVRS
                     DDMGATWNEIFGIDYKTAAYSYYTWGVAGCNIGETMWVSATPTTYVGSFVFRTTDGGA
                     NWDSFEIPSDIIATYPRAIAFATAEKGLIADRRGNVVASVDGGATWYKTNKPDTSADS
                     WCNGVVHIPGTEMIMGMDDLGVYVTSDLGDTWSRLNTPQDITDAGIYYTAGVFLNQNF
                     GYVFGDKGTILRFANQLTGVADRPRESMPDNFQLSQNYPNPFNPTTTISYTLPARQNV
                     SLAIYNLKGELVKSLVDGPMSEGSHAVVWDATNTLGQKVTSGIYIYLLRSDNRQIARQ
                     MVLVK"
     gene            complement(4122..>4542)
                     /locus_tag="PKM23_12295"
     CDS             complement(4122..>4542)
                     /locus_tag="PKM23_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92295.1"
                     /translation="ILHFGPEVSFSGSYIDFSDSKSYSVNKSKEFNTYSLPINFRFGA
                     AIDLLNNDQHRLTTAIDMVHPNNNLEQYNMGLEYGFNQMFFLRGGYQIEADEGGLGLG
                     LGVQTPVSGQLGLVFDYSYADLGILTTSHRFSLSVKY"
BASE COUNT          963 a         1318 c         1335 g          926 t
ORIGIN      
        1 atttccgcaa gtatgaatcc ctcagcatcc agtattcgcg cggctgcccc tttcagtgcg
       61 atttctgcaa tatcaccgcc atgctcggcc gccggccgcg caccaagcgc gccgcgcaaa
      121 tcatcgccga actggacaag atctaccggc tgggctggcg gcgcaacgtc ttttttgtcg
      181 atgacaactt tatctccaac aagcgccagc tcaaggagga gatcctgccc gcccttatcg
      241 cctggcgccg gggcagggcc ggttgcagct atctcaccga ggcctcgatc aacctggctg
      301 acgacgagga gctgatgggg atgatgtccg cggccggatt caactcggtc ttcgtcggca
      361 tcgagacccc ggacgaggcc agcctggcgg agtgcaacaa ggggcagaat gtccatcgca
      421 acctggtgga gtgcgtgcag gccatgcagc gccacggtct gcaggtcatg ggtggattca
      481 tcgtcggctt tgacaacgac accgagtcga ttttcgatcg ccagatcgaa ttcatccaga
      541 agagcggggt ggtgacggcg atggtgggga tgcttcaggc catccccggc actcctctct
      601 ttgcacgcct tcatgccgag ggcaggatca cccacgagat gaccggggac aatgccgacg
      661 gcacgaccaa tatcatcccg gccatggggc tggataaact gaaaacggga tacgcccggc
      721 tgatggctga actctacagc ccggaaaatt tctatgcccg catccggacc tgcctgcgtc
      781 tctaccatcc ccccaaagcc ccggtccgcc tgcacgctga ggagctctat gccttcttgc
      841 gctcgatctg gaagctcggc accctcagcg cggagcgaaa ccattactgg cgcctctttt
      901 tctggactct tttccaccgc ccgcgcagcc tggccctggc ggtcaccttc acgatctatg
      961 gttttcattt ccggacggtc aatgccctga atgggatacg ctgagcagcc ggggggcgaa
     1021 aaatggctct tttttgactt tttccttgat tttaaatgat gttaaaggta tattgcctta
     1081 cgtctttatg ccactggaaa cccacatagc agaaaatctt tatccaacct gtgattacca
     1141 gaaaaacagt cagggtttac aaaccaaccc aacatcccac taacaacaag gaggttacca
     1201 tgaggagcag gaccgttact atgttgttga tgtttgcggt tttggtgttt tttgcggttg
     1261 ggagcagctt cgcccagggg cagttcgtcc gggctggcga gatccctgtg cctgaaacgg
     1321 acctcaacaa cggcggcacg ggcaacatga tcgccggtgt ggatttcgac aacgacggca
     1381 agaccgaaat ttatctggtc aatgacaact ggaatgatac cccgaccgag gtgatccccc
     1441 gcatctacaa gctggaaaaa gagggcagcg aatgggtcgt ggtctggaag gctgttgccc
     1501 cgatcgagaa gcagaacacc tggcccttcc tgacccacgg ggatctggac aaggacggca
     1561 agcacgaaat ctactggggc gtggtaaaca atctctccgg tacaactggg cttaatcctt
     1621 accggattct ggtttatgag tccaagggcg atggcagcga tgtcatgggc gtcgatgacg
     1681 gcgtcggcgg ctacctgccc aatgccaagt ggaccatctc cgcggccgac aacgacaata
     1741 tccgcccgat ccaggcctgc atcgccgatc cggacaacga cggcgttgac gagttgctct
     1801 tcgcagaccg tgccggccgc accggcggcg tcttctgcgg catcgtctcg gtcgacaaga
     1861 ttcccgataa cggcgacggc accgaggtat ggaccatgga gaccagcggc ctcgatttta
     1921 ccctcaaggc cggcacgacc cagaataaat gggatgtggt tgccatcggc gccaacgtct
     1981 acagcttttg cgaagtcgag gtcaccaagt tcacctggga cggtttcggc tggggttata
     2041 ccaaactctc cccgctgctc ggcggtgcac cggtgcagtc gacccaggtg gtcgatctca
     2101 acggcgacgg cgtcaaggag atgatcgtcg cggtctacga ctggggcaac gatgccgcca
     2161 agggcatcta tctggttcag gaagacggcg acacgctcaa gaacaccgag ctggtcaatg
     2221 tgggcaaatg gtggccgagc ggctcgcgcg gcctttgggg cggtgattca ggcgatattg
     2281 acggcgacgg caagctcgat ttcgtttacg gctcgcgcgc ttcgaccccc aatgccggca
     2341 tcttccatct cgcctacaag ggcggcgaca tcgccgatcc ggccagctat accttcaaca
     2401 tgatcgattc cctctatgcc gagggcggca tctggaccgt ggtcaagctg gccaatgtcg
     2461 acagcgatcc ccagctcgag gtgctctata cctccagcac cgacgccggg gccttcccgg
     2521 aactgggcac caagccgatc gtcgtgctcg actatgtcgg cggcggcgcg ccagtctttg
     2581 atgatctggt tgtagcgacc gaggtcaagc tgaacggcga agcgccgacc gggctgctct
     2641 tcaagccggg gcggattctg gataacggcc ggacgatctg gttctgtgga gtggatggca
     2701 ccaataaggt gacctgggta ttccgctcgg ttgatggcgg ccagaccttc acccacaacg
     2761 ccactgctct tgtcgaaaga gccgcgcaga tggatgcctg ggatgccaac accgccgtta
     2821 ttgcccaggc cgacggcaag atctatcgca ccaccgacgg cggcgtcaac tggaccgagg
     2881 tctattcata cacgatcagc ccgatcgcac cggggtggtt tgacggcctc cgggtgatca
     2941 acgggaccag cgccatcgcc tttggcgaca tggagccgga cggcaacatg cactttgtgc
     3001 gttcggatga catgggggcg acctggaacg aaattttcgg catcgattac aagacggcgg
     3061 cctattccta ctacacatgg ggcgtggccg gctgcaacat cggtgagacc atgtgggtga
     3121 gcgccacgcc gaccacttat gtcggttcgt ttgtattccg cacgaccgac ggcggcgcca
     3181 actgggacag ctttgagatc cccagcgaca tcatcgccac ctacccccgc gccatcgcct
     3241 tcgcgaccgc ggagaagggc ctgatcgccg accgccgcgg caacgtggtg gccagcgtgg
     3301 atggcggcgc cacctggtac aaaaccaaca aaccggatac ttccgcggat agctggtgca
     3361 acggtgtggt gcacattcct ggcacggaga tgattatggg catggatgac ctgggcgtct
     3421 atgtcacatc tgatctggga gacacctgga gccgtctgaa caccccgcag gatatcaccg
     3481 atgcgggcat ctactacacg gcgggtgttt tcctgaatca gaattttggc tatgtctttg
     3541 gggacaaggg cacaattctg cgctttgcca atcagctgac cggtgtcgcc gatcgtcctc
     3601 gcgagagtat gccggataat ttccagctaa gccagaatta tcccaatccc ttcaatccga
     3661 ccacgaccat cagctacacc ctgcccgcgc ggcagaatgt gtcactggcc atttacaatc
     3721 tcaagggcga gctggtgaag agcctggtgg atggtccgat gagcgagggc agccacgcgg
     3781 tggtttggga tgcaaccaat acgcttggcc agaaggtgac gagcggcatc tacatctatc
     3841 tgctgcgctc cgacaaccgt cagatcgcgc gccagatggt gctggtcaag taaattaacc
     3901 ctgttttcaa aaacctggcc ggattatccg gccaggtttt tttatggcca taggcaaagt
     3961 tggcgtcgag cgggcgcagg atataaaaga gcccccggct gaatctccag ccgggggctc
     4021 ttttatgccg ggatgaaggc gcagtgcaag ctttacgccc gaaagtgacc tgtaataaaa
     4081 aagcctcctg caaggaggct tttttataaa tcaagcggag cctagtattt caccgagagc
     4141 gaaaagcggt gcgaggtggt gaggatgccc aggtccgcat aggagtaatc gaagaccagg
     4201 ccgagctggc cgctcaccgg cgtctgcacg cccaggccga ggccaagtcc gccctcatcc
     4261 gcttcgatct ggtagcctcc gcgcaggaag aacatctggt taaagccgta ctccagcccc
     4321 atgttgtact gctcgaggtt gttgttggga tggaccatat cgatggctgt ggtcaggcgg
     4381 tgctgatcgt tgttgagcag atcgatggcc gctccgaagc ggaaattgat cggcagcgaa
     4441 taggtattga actccttgct cttgttgacg ctgtaggatt tggagtcgct gaaatcgata
     4501 tagctgccgc tgaaactcac ctccgggcca aagtggagga tg
//
LOCUS       DAOPWU010000287         4495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19418_length_4495_cov_4.395045, whole genome shotgun sequence.
ACCESSION   DAOPWU010000287 DAOPWU010000000
VERSION     DAOPWU010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19418_length_4495_cov_4.395045"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKM23_12300"
     CDS             <1..552
                     /locus_tag="PKM23_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92296.1"
                     /translation="IMAPADMAAAAWEFFRQEKIGQAVAMFEALRQDQAQSFEAFTGL
                     GWCYAHPLVDSLEKAAGYFSGSLILQAINADACAGRGFVRLALNQYTQAIADFEKVLT
                     INPSYNFAYNPRVNAASVRLGLAEAHFYLQNFSAAQGQIDRLASGNGLQPGQPSTWSV
                     EGKSYGSYPEALLAWIEKLKAQG"
     gene            complement(684..2528)
                     /gene="typA"
                     /locus_tag="PKM23_12305"
     CDS             complement(684..2528)
                     /gene="typA"
                     /locus_tag="PKM23_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNY92297.1"
                     /translation="MKRTASNPLIRNIAIIAHVDHGKTTLVDQMFRQSGLFRDNQQVE
                     ERIMDNMDLERERGITIAAKNCSVSWEGIKINILDTPGHADFGGEVERALKMVDGAIL
                     LVDSSEGPLPQTRFVLKKALEENLKIIVVINKIDRKDARPQQVLNEIFDLFIDLDASE
                     EQIEFPVLYAIGRQGIVQSSLTEPGSDLRPLFAAILEYIPPPCYDPEEPFQMLVSDLN
                     YSDYLGRLAIGRVFNGSAHNNDALICITEDKGEIPLRVTRIQVYDGITLRETDLAQPG
                     DIINLAGIEEVHIGDTICTAQAPKPLKRITVDEPTISMLFTINTSPLSGREGRHVQSG
                     KLRERLMKEVLRNVSIRVEAGESPDRFIVKGRGEFQMAILIEQMRREEFELCVGRPSV
                     IFRTIHGVRHEPIEHLFIDCEEQFLGIVTEKLSRRKARMLNLVNHGTGRVRVEFSIPS
                     RSLIGYRNEFLTDTKGTGIMNFLLDGYEPYRGDFVSRLTGSIVSDRSGETVPYALGNL
                     EPRGILFVGPNEPTYEGHIIGEHNRENDIDVNPTKQKKLTNMRSSTKDEAVQLTPFTP
                     LTLERAIEFIKDDEMVEVTPKSIRLRKAILTAQQRKDYERQLSMAESA"
     gene            complement(2754..>4495)
                     /locus_tag="PKM23_12310"
     CDS             complement(2754..>4495)
                     /locus_tag="PKM23_12310"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92298.1"
                     /translation="ALNSLQIIYIPNPETVDSMLIQVVAKVPPEYTPPEEAFIVTPLE
                     KHHLDTPTVITKGYGYHYEKMLKPAAFIKVYMEGDANPGYSCARAAVVYILRKSSEAR
                     FTTGKLVHQGLWWEAGDRPNTWSEIQTIPATFGPRDVEITCVITDKESSSRLALLRAE
                     AGPRVVEKSIDDPNFGDEVLVETLLLKAVPGEVTEVKVTVSSPEDKGDSIFWSGMVLS
                     TSCGEVDMGDAMEHGFPTLFSHDGARHTYRPGYFLGAAIDTEPDGHPGDKADGDDTIG
                     IDDEDGVEFKSKFYQGQAGQMEITASAPGYLNVWFDWNGDGTWSQDGEHAVVDLALST
                     GVNPLSIDVPMTGLEELFSFCRFRFSSVPGLLASGPAPDGEVEDYLVPVYTPVEMSSF
                     FAAAMDGAVVLQWETQSETSNLGFNVYRATEEGGIFSQINLRMIQGAGSSASAHRYRY
                     VDETAEAGKVYYYQIVDIAADGQMQWHGPVMVEVAKPTANRLDQNSPNPFNATTRISY
                     SIKQPGQVVLAIYNLQGQRVRTLVAKPHETGSYSAIWDGRDDAGHDLPTGAYLYLLKM
                     PDAELSQRMTLIK"
BASE COUNT          801 a         1289 c         1455 g          950 t
ORIGIN      
        1 attatggctc ctgctgacat ggctgcagct gcctgggagt ttttccggca ggagaagatc
       61 ggccaggcgg tggccatgtt cgaggccctc cgccaggacc aggcgcagtc cttcgaagcc
      121 ttcaccgggc tgggctggtg ctacgcccat ccgctcgtgg acagcctcga aaaggccgcc
      181 ggctatttca gcggctccct gatcctgcag gcgatcaacg ccgacgcctg cgccgggcgc
      241 ggctttgtga ggcttgccct caatcagtac acgcaagcca tcgccgattt tgaaaaggtc
      301 cttaccatca atccctcgta taactttgcc tacaaccccc gggtcaatgc ggcttcggtg
      361 cggctgggac tggcggaggc ccatttttac ctgcagaact tttctgccgc ccaggggcag
      421 atcgatcggc tcgcctctgg caacgggctg cagcccggac agccctccac ctggagcgtg
      481 gagggcaagt cctatggcag ctatcccgaa gccctgctcg cctggataga aaaactcaaa
      541 gcccagggat gagccctcgt cttgaggaat gaaaaaagcc ctgacggtca tgccgtcagg
      601 gcttttttcg tttgtcccgc aggggtggcg gtcacgaccg gggcagcgcc gcgcggctat
      661 ggcctccaac ggcgccaaaa cgatcaggcg gactcggcca tgctgagctg gcgctcatag
      721 tccttgcgct gctgggccgt gaggatggcc ttgcgcagcc ggatcgattt cggggtcacc
      781 tcgaccatct cgtcatcctt gataaattct atcgcccgtt caagggtcag gggtgtgaag
      841 ggtgtgagct ggaccgcttc atccttggtc gagctgcgca tgttggtcag ctttttctgt
      901 ttggtgggat tgacgtcgat gtcgttttcg cggttgtgct cgccgatgat gtggccctca
      961 taggtgggct cgttggggcc gacgaagagg atgccgcgcg gctcgaggtt gccgagggcg
     1021 tagggtacgg tttcgcccga gcggtcggag acgatcgaac cggtcagacg tgagacgaaa
     1081 tccccgcggt agggctcata gccgtcgagg aggaaattca tgatgccggt gcccttggta
     1141 tcggtcagga actcgttgcg gtagccgatg agggagcgcg aagggatgga aaactcgacc
     1201 ctgacccggc cggtgccgtg gttgaccagg ttgagcatgc gcgccttgcg ccgcgagagc
     1261 ttttcggtga cgatcccgag gaactgctcc tcgcagtcga tgaacagatg ttcgatcggt
     1321 tcatggcgga cgccgtggat ggtcctgaaa atcacgctcg gccggccaac gcagagttcg
     1381 aactcctccc gccgcatctg ttcgatgagg atggccatct ggaactcccc gcgccccttg
     1441 acgataaagc ggtcggggct ctcgccggcc tcaacccgga tcgagacatt gcgcagcacc
     1501 tccttcatca agcgctcgcg cagcttgccg gactgcacat gacgcccctc gcgtccggag
     1561 aggggggagg tgttgatggt gaagagcatc gagatggtcg gctcgtcgac tgtgatgcgc
     1621 ttgaggggct tgggcgcctg ggctgtgcag atggtgtcac cgatgtgaac ctcttcgatc
     1681 ccggccaggt tgatgatgtc gccgggctgt gccaggtcgg tctcgcgcag ggtgatgccg
     1741 tcatagacct gtatccgggt gacgcgcagg gggatttcac ccttgtcctc ggtgatgcag
     1801 atcagggcgt cattgttatg cgcgctgccg ttgaagaccc ggcctatggc caggcggccg
     1861 agatagtcgg agtagttgag gtcagagacc agcatctgga agggctcctc cggatcgtag
     1921 caaggaggtg gaatatactc gaggatggcg gcgaagaggg gacggaggtc gcttcccggc
     1981 tccgtgaggg agctctggac aatgccctgg cggccgatgg cataaaggac cgggaattcg
     2041 atctgctcct cggaggcatc gagatcgata aagaggtcaa aaatctcgtt gaggacctgc
     2101 tgcggccggg catccttgcg gtcgatcttg ttgatcacca cgatgatctt gagattttct
     2161 tcgagggcct tcttgaggac aaagcgagtc tgcggcaggg gcccctcgga ggaatccacc
     2221 aggaggatgg cgccgtcgac catcttgagg gcgcgttcga cctcgccccc gaaatcggcg
     2281 tggcccgggg tgtcgagaat gttgatcttg atcccctccc agctgaccga gcagtttttc
     2341 gccgcaatcg tgatgccgcg ttcgcgctcg agatccatgt tatccatgat gcgctcctcg
     2401 acctgctggt tatcgcggaa gagcccgctc tgtcgaaaca tctgatcgac gagggtggtt
     2461 ttgccgtgat cgacatgggc gatgatggcg atattgcgga tcaggggatt ggatgcggtc
     2521 cttttcatgc tttccttctt ttgctgggtt acgatcgggg cggatacggc gcgtgccggc
     2581 cggagttttg aatagcttaa aatatacgca catattttgc tatttactac ggaaaaaatg
     2641 gcccttttgc cgtccggctc cactcctgct gtctcgacag ggcataaaaa agggcggacc
     2701 ctcgtgggga tccgcccttg tccaacagaa aaccggctgc ccggccaacg ggatcacttg
     2761 atcagggtca tcctctggct cagctcggca tcaggcattt taagcaggta gagataggcc
     2821 ccggtgggca gatcgtgccc ggcatcatcg cgcccatccc agatcgccga gtagctgccg
     2881 gtctcgtgcg gcttggcgac cagggtgcgc acgcgctgac cctgcagatt gtagatcgcc
     2941 agcaccacct gtccgggctg cttgatggag taggaaatgc gcgtcgtggc attgaatgga
     3001 ttgggtgaat tttgatcgag gcggttggct gtcggcttgg ctacttcgac catgaccggt
     3061 ccatgccact gcatctgtcc gtcagcggcg atgtcgacga tctggtaata gtagaccttg
     3121 cccgcctccg cggtctcatc cacatagcgg tagcggtgcg cgctcgcact gctgcccgcc
     3181 ccctggatca tccggaggtt gatctgggaa aagatgcccc cctcctcggt ggcgcggtag
     3241 acgttgaagc ccaggttgct ggtctcggac tgggtctccc actgcagcac gacggcgcca
     3301 tccattgccg ccgcgaagaa ggaactcatc tcgaccgggg tgtagaccgg caccagatag
     3361 tcttccacct cgccgtccgg cgcggggccg gaggccagca ggccgggcac cgagctgaag
     3421 cggaagcggc aaaaagaaaa gagttcctcc agcccggtca tcggcacatc gatgctaaga
     3481 ggatttactc ctgtactgag ggcgagatcg accacggcgt gttcaccatc ctggctccag
     3541 gtgccgtcgc cgttccagtc aaaccagaca ttgagatagc ctggtgccga tgcggtgatt
     3601 tccatttgtc cggcctggcc ctgatagaat ttgcttttga attcgacccc gtcttcatcg
     3661 tcgatgccga tggtgtcatc cccgtcggcc ttgtcgccgg ggtggccgtc cggttcggtg
     3721 tcgatggcgg cgccgagaaa atagcccggt ctataggtat ggcgcgcccc gtcatggctg
     3781 aacagggtgg ggaagccgtg ctccatcgca tcgcccatat cgacttcgcc gcagctggtc
     3841 gagagcacca tgccactcca gaagatcgag tcccctttat cctcgggcga ggagaccgtc
     3901 accttgacct cggtgacttc accgggcacc gccttgagga ggagggtctc caccagaacc
     3961 tcgtcgccga agttgggatc gtcgatactc ttttcgacga cgcgggggcc ggcctcggcg
     4021 cgcaggaggg cgagccggct ggagctctcc ttgtcggtga tcacgcaggt aatctcgacg
     4081 tcgcgcggcc cgaaggtggc cgggatggtc tggatttcac tccaggtgtt ggggcgatcg
     4141 ccggcctccc accacagccc ctgatggacg agcttgccgg tggtgaagcg ggcttcagag
     4201 cttttgcgca gaatgtagac gaccgcggcg cgggcgcagc tgtagccggg gttggcatcc
     4261 ccctccatgt agaccttgat gaaggcggcg ggcttgagca ttttctcata gtgatagcca
     4321 tagcctttag tgatgaccgt cggggtgtcg agatggtgct tctccagcgg cgtgacgata
     4381 aaggcctcct ccgggggggt gtactcgggc ggcaccttgg cgacgacctg gatcagcatg
     4441 ctgtcgacgg tttcaggatt ggggatgtag atgatctgga gactgttcaa ggctg
//
LOCUS       DAOPWU010000288         4483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19489_length_4483_cov_5.713415, whole genome shotgun sequence.
ACCESSION   DAOPWU010000288 DAOPWU010000000
VERSION     DAOPWU010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19489_length_4483_cov_5.713415"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..336
                     /locus_tag="PKM23_12315"
     CDS             85..336
                     /locus_tag="PKM23_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNY92299.1"
                     /translation="MCIAAPGRVIRLYQREGLEMGEVDFGGIAREVCLAHVPGVRAGD
                     HVMVHAGFALEIIDESEVARFQQLWREVLTGGRAGEESP"
     gene            333..1460
                     /gene="hypD"
                     /locus_tag="PKM23_12320"
     CDS             333..1460
                     /gene="hypD"
                     /locus_tag="PKM23_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNY92300.1"
                     /translation="MNLAPFRDPAIAARLIEETHRVQSRPWVIMETCGGQTHAILRLG
                     LDQLLPRGLELVHGPGCPVCVTPAPLIDHAIALAARPEVILATFGDMMRVPGTASTLF
                     AARAAGGDVRMVYSALDALELAHTNPDREVVFFAIGFETTAPGSAQAVMRARSQGVAN
                     FSLLSAHVLVPAAIRYLLEDPGNRVQGYLAAGHACAVLGGQPYEELAARFDVPIVITG
                     FEAIDLLRGILRVVQMLEAGEARVENAYERVVQNEGNLQAQAAIRNVFMSRGREWRGI
                     GLIPASGLTLRPEYSAWNAQLKFGPPAATSTEPGVCISGLVLQGLKKPPECPAFGREC
                     TPGHPLGAPMVSAEGACAAYFSYQRMPEGRDDKSPGSEGAA"
     gene            1457..2470
                     /gene="hypE"
                     /locus_tag="PKM23_12325"
     CDS             1457..2470
                     /gene="hypE"
                     /locus_tag="PKM23_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011769605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNY92301.1"
                     /translation="MNNERIQLGHGSGGRMMNELIHDLFRRRFSNPQLNALDDGAILP
                     EPGGRLAFATDSFVVDPLFFPGGNIGDLAVNGTINDLCMCGSRPLWLSAGFIIEEGLA
                     CSDLEIIVGAMAAAAAAAQVRIVTGDTKVVERGKADRLFINTSGIGVISHNPAPGSCR
                     IEPGDRLILSGTLGDHGIAVLSQRSHLGFSTPLQSDTAPLHELTARLLEACGGGVHAM
                     RDPTRGGLAALCNEFARSAGVEIRLDETALPVRPEVAAACELLGLDPLYIANEGKLVA
                     AVAADAAERALGALKSHPLGREAAVIGEVRKGEAGLVAMRTILGGWRVVDLPAGELLP
                     RIC"
     gene            complement(2575..4041)
                     /locus_tag="PKM23_12330"
     CDS             complement(2575..4041)
                     /locus_tag="PKM23_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNY92302.1"
                     /translation="MISKEFFKSSLVYSVVGALPLAASVLLLPFYTNLLSTADFGRLA
                     LYISFSFLIQILVSFGLDSYITVQYIEFKFDPALLRENMSAITTSFLVISAVFTLLSL
                     FTGPVLFSLFFRDGSLSFYPWGFLSVLTAVFHGAFKLYTNLLIYQQRPGRYFWVNIIN
                     FLLVIGISLGGLYLFPRSLVGPMWGRLLSALALAAFSIHYLLRSYGVARGRAFIKGMV
                     SYCSPLVIYSLLFWVLSYVDRFVINHFMRAEDVAVYDFAVKCTLLIEFFQTGLVSAIY
                     PKVFGIWKEQKVPESSVQVNRYFNGFSATTLLVLPLFLMVVPALVPLVVHEEAYYAGF
                     KFLPLLSLGFIGRTLFYMFLAPFYYFRKTRLLPRVLFFSSLLQLLLSVWFVKRWGLAG
                     AVWINFIMKWVVAGFAFNECRKIFKFKFNRIKLIWLPLVYTLTFILAGRAAGPDDALL
                     VYFGEALVSLALVVFVYRRELLLLLEQWRRKNRLSTAG"
     gene            complement(4181..>4483)
                     /locus_tag="PKM23_12335"
     CDS             complement(4181..>4483)
                     /locus_tag="PKM23_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92303.1"
                     /translation="VEVYNQGVRYAGDKRYDQAEKAFLRVIKEGGNTELAGLARTNVT
                     KIRIARQAAWYNEAFDLMRANAFDKAVPLLKKVIRDNSDPQTTRAAENALSQIEAL"
BASE COUNT          898 a         1364 c         1473 g          748 t
ORIGIN      
        1 ctcggtcagg cggcggtcgc agccgcgcag ctgcgcaaga gctgaagccc ttgctgaatc
       61 gaatcatcca gagaggaaaa acctatgtgc atcgccgccc ccggcagggt gatacgccta
      121 tatcagcgtg aaggcctcga gatgggagag gtcgatttcg gggggatcgc cagggaggtc
      181 tgccttgccc atgtgccggg tgtgcgcgcg ggggaccacg tcatggtgca cgctggcttt
      241 gccctcgaga tcatcgatga gagcgaggtg gcccgcttcc agcagctctg gcgggaggtg
      301 ctcacgggcg gccgggctgg agaggagagc ccgtgaacct cgcccccttt cgcgatcccg
      361 ccatcgccgc ccggctcatt gaggagactc accgcgtgca gagccggccc tgggtcatca
      421 tggagacctg cggcggccag acccacgcca tcctccgcct cggcctcgac cagctgctgc
      481 cgcgcgggct ggagctggtg cacggaccgg gctgcccggt ctgcgtgacc ccggcgcccc
      541 ttatcgatca tgcgatcgcg ctggctgcgc ggccggaggt gatcctggcc acctttgggg
      601 acatgatgcg cgtgcccgga acagcctcca ccctcttcgc ggcgcgcgct gcgggcgggg
      661 atgtgcgcat ggtctactcc gccctggacg ccctggagct ggcgcatacc aatccggatc
      721 gcgaagtggt ctttttcgcc atcggcttcg agaccaccgc cccgggcagc gcccaggccg
      781 tcatgcgcgc ccgcagccag ggcgtcgcca atttctcgct gctctccgcc catgttctgg
      841 tccccgccgc catccgctat ctgctcgagg atccgggcaa ccgggtgcag ggctatctcg
      901 ccgcgggcca cgcctgcgcc gtcctgggcg ggcagcccta tgaagaactc gccgcccgat
      961 ttgatgtgcc catcgtcatt accggctttg aggccatcga tctgctgcgc ggcattttgc
     1021 gggtggtgca gatgctggag gcgggcgagg cccgggtcga gaatgcctac gaacgcgtgg
     1081 tgcagaatga gggcaatctc caggcgcagg ctgccatccg gaacgtcttc atgagccggg
     1141 gccgcgagtg gcgcggcatc ggcctcattc cggccagcgg cctcaccctg aggccggagt
     1201 acagcgcctg gaatgcgcag ttaaaattcg gaccaccggc ggcgaccagc accgagccgg
     1261 gagtttgcat cagcgggctg gtcctgcagg gactgaaaaa acctccggaa tgcccggcct
     1321 tcggccgcga gtgcaccccc ggccaccccc tcggcgcccc gatggtctcg gcagaggggg
     1381 cctgcgccgc ctacttttct tatcagcgca tgccggaagg aagggatgac aaaagccctg
     1441 gatcggaagg tgcagcatga acaatgagag aatccagctc ggccacggca gcgggggccg
     1501 gatgatgaac gagctcatcc acgacctctt caggcgccgg ttttccaatc cccagctcaa
     1561 tgccctcgat gacggcgcca tcctgcctga gcctggcggc cgcctcgcct tcgccactga
     1621 cagctttgtc gtcgatcccc tcttctttcc gggcggcaac atcggcgatc tcgcggtcaa
     1681 cggcaccatc aacgatctct gcatgtgcgg cagccgcccc ctttggctca gtgccggttt
     1741 tatcatcgag gaagggctgg cctgcagtga cctcgagatc atcgtcggcg ccatggctgc
     1801 ggcggcagcc gcggcgcagg tgcggattgt gacgggggat accaaggtag tcgaacgcgg
     1861 caaggccgac aggctcttca tcaacaccag cggcatcggg gtgatcagcc ataatcccgc
     1921 gccgggctcg tgccggatcg agccgggcga ccggctcatc ctcagcggca ccctcggcga
     1981 ccacggcatc gccgtcctct cgcagcgcag ccatctcggc ttttcaacac ccctgcagag
     2041 tgacaccgcc cccctccacg aactgacggc ccgcctgctc gaggcctgcg gcggcggagt
     2101 gcacgccatg cgcgatccga cccgcggcgg attggccgcc ctctgcaatg agttcgcccg
     2161 cagcgcgggc gtggagatca ggctggatga gacggccctg ccggtgcggc ccgaggtggc
     2221 ggccgcctgc gagctgctcg gcctcgatcc cctttacatc gccaatgaag gcaagctggt
     2281 ggccgccgtg gccgccgatg cagcggagag agccctcggg gcgctgaaaa gccatcccct
     2341 cggccgtgag gccgccgtga tcggcgaggt gcgaaagggg gaagcgggac tggtggcgat
     2401 gaggacgata ctggggggat ggcgcgtggt cgacctgcca gccggagagc tgctgccaag
     2461 gatctgctga gccctccgcc ggggatcaag ctgtccgatt tgccgcacgg agagggagct
     2521 gccctggagg tgatgatgtt gttgagccgg caggcgtggg taaacgcgcc cgcttcagcc
     2581 ggccgtggag agccggtttt tcctccgcca ctgctccagg aggaggagca gctcgcgccg
     2641 gtagacaaac acaaccaggg cgaggctgac cagggcttcc ccgaaataaa ccagcagggc
     2701 atcgtccgga ccggccgccc ggccggcgag gatgaaagtc aatgtataga ccaggggcag
     2761 ccagatcagc ttgatccggt tgaatttgaa tttaaagatc ttccggcatt cattgaatgc
     2821 gaatcccgcc accacccact tcatgataaa attgatccag actgcacccg caagtcccca
     2881 cctcttcaca aaccacaccg aaagcagcag ctgcagcagg gatgagaaaa acagcacccg
     2941 cggcaggagc ctggtcttgc ggaagtagta gaagggagcg aggaacatgt aaaaaagcgt
     3001 gcgcccgatg aagccgaggc taagcagggg aagaaattta aagccggcat aataggcctc
     3061 ttcgtgaact acgaggggga ccagcgccgg caccaccatc agaaaaagag gcagcaccag
     3121 cagtgtggtg gcggagaagc cgttgaaata acggttgacc tgaaccgagc tttccgggac
     3181 cttctgctcc ttccagatgc cgaagacctt gggatagatg gcgctgacta gtccggtctg
     3241 aaaaaactcg atgagcaggg tgcatttgac cgcaaaatca tagaccgcca catcctcggc
     3301 gcgcatgaaa tggttgatga caaagcggtc gacataggaa aggacccaaa agagcagcga
     3361 gtagatgacc aggggcgaac agtaggagac catccccttg ataaaagccc tcccccgcgc
     3421 caccccgtag ctcctcagca gataatgaat tgaaaaggca gccagggcca gggcggagag
     3481 cagccgcccc cacatcggac cgaccaggga gcgggggaag agatatagcc cgcccaggga
     3541 aatgccgatg acgaggagaa aattgatgat gttgacccag aaatagcggc cggggcgctg
     3601 ctgatagatg agaagattgg tatagagctt gaaggcgccg tgaaagaccg ctgtcagcac
     3661 cgacagaaaa ccccagggat aaaagctgag cgacccgtcc cggaagaaga ggctgaaaag
     3721 caccggaccg gtgaaaagcg agagcagggt gaagactgca ctgatgacca gaaaggaggt
     3781 cgtgatggcg ctcatgttct cgcgcaggag agcgggatcg aacttgaatt caatatactg
     3841 gaccgtgata taggagtcta gtccgaaact gaccaggatc tggatgagga aggagaagct
     3901 gatatagagg gccaggcggc cgaaatcggc tgtgctgagc agattggtgt aaaaggggag
     3961 cagcaagacg ctggcggcca ggggcagcgc gccgacgact gaatagacca gtgaggattt
     4021 gaagaactct tttgagatca tcgctctggc ggttccggat gagggggatt aacggctgcc
     4081 tgcacgggat cggattcagg gaggcgcctg tttccgccgt caggttcggc tgctgcggaa
     4141 tttccaggca cggccgctgc gcgggggctg ccgtgcacaa tcaaagggcc tcgatctggc
     4201 tcagcgcatt ttcggcggcg cgtgtggtct ggggatccga gttgtccctg atcacctttt
     4261 tgagcagggg caccgccttg tcgaaagcgt tggcgcgcat gaggtcaaag gcctcgttgt
     4321 accaggcggc ctggcgggcg atgcgaatct tggtgacgtt ggttctggcg aggccggcga
     4381 gttcagtatt gcccccctcc ttgatcaccc gcaagaaggc cttttccgcc tgatcgtagc
     4441 gcttgtcccc ggcgtagcgg accccctgat tgtagacctc gac
//
LOCUS       DAOPWU010000289         4480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19510_length_4480_cov_3.516384, whole genome shotgun sequence.
ACCESSION   DAOPWU010000289 DAOPWU010000000
VERSION     DAOPWU010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19510_length_4480_cov_3.516384"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1923)
                     /locus_tag="PKM23_12340"
     CDS             complement(<1..1923)
                     /locus_tag="PKM23_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNY92304.1"
                     /translation="MAFVNGPINRPILTSVIFLVIIVLGVVSFSRLSIDLMPEITYPT
                     ISVVSDYGNVGPQEMEELITRPLEETLAAVQGVEEITSTSSEGRSVVRCSFAWGTDLD
                     VASNDIRDRLDRALGRLPEDMERPMIRKFDISATPIMFMGVTSDMHPLELRQLVENQV
                     KYRVERVPGVAAVNIWGGLYREIHVDLRADQLKALGLSTNAIINALRSENRTIPAGLF
                     KKGNLEIQIRTQGEYVNLEEIRNTVITTRQGTPIRISDVANVEDSWQEVRSLNRLNGK
                     AGLRMAINKQSGSNTVEVADAVNKEIERINLDLPQIKITPNFDSSVFIKQSINSVANS
                     TLFGGLLAIVILLLFLRNLSSTSIIATAIPISIVATFGLMYFNGFTLNSITFGGLALG
                     IGMLVDNAIVVLENIYRYRENGLKAIEAARKGAGEVSGAIIASTLTTIVVFFPVIFIR
                     GMSGIMYQQMAYVVVFSLLCSLLVALTLVPMLSSRFLRYHPVQHGKKISRIEKIYTVS
                     QELFEKLERAYSGLLRKALGRRKLVIWLALGLFAFSVLLVRFIGVELMPQTDEGEVNV
                     SLEAEIGSRLEVVDRIALQVEEIVRREVPEMTTMMARVGEGNSADIRLSLVPKDQRKR
                     SSEEVANALRKATAEIP"
     gene            complement(1948..3129)
                     /locus_tag="PKM23_12345"
     CDS             complement(1948..3129)
                     /locus_tag="PKM23_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNY92305.1"
                     /translation="MNKRRILFLLAALFLLFLLWRIFTLIFSGGEKAKQGGGQLPVAV
                     EADTVRHEAIEEIRQFTGSVYPIYQYVLAPKVSGRLMTIRKRIGDWVRAGEVVAVIDD
                     AEYQQALREAEANLKIAEASLIEAESQALLASQELERVRLLKEKGIAAQAEFDVASSN
                     EVARKSRLELARAQVEQRQAALKSAQIRLDYTRLNASEPGFIGERFVDEGALLTVNSA
                     VVSVVGIDRVIVRTTIVERDYGHIQVGQAARVYVDTWPGRGLEGTVARIAPVLDEAAR
                     VAKMEVEVENGERLLKPGMFARAMVTLRSSARAQTVPSKAIVNREGASGLFLVSADKK
                     SARYVPVEIGIVSGERTEILSPRLEGRVITLGQHLLQEGSLIILPGAGNGAPGTSPAG
                     N"
     gene            3435..>4480
                     /locus_tag="PKM23_12350"
     CDS             3435..>4480
                     /locus_tag="PKM23_12350"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNY92306.1"
                     /translation="MRAFRLTLLLFFLLLQAFGADSALALDPGKLITQYDIRNYTAKD
                     GLPMNTVKKVFQDSRGYIWVGTQEGLARFDGVRFRIYDKSTSPGLRSSFIRDIAEDHA
                     GHLWVATNGGGISRLEGDDFVSYGAAEGLNHDVVNTLLVARDGSLVIGTENGISRFAD
                     GRLTTWIFTDNVKANNIQALIQDSTGVLYVGRYGALLTRIAWADFTAASGTPQSGVTY
                     CRVPLPARRTEISPFEESFTALCTTRAGDLLAGNTNGNVGWVAPAAGGALAPFWSPDS
                     PRDQITVRALLEDRDGLVWICTEGRGIVRWHQGESSRLDLEQGLATSGDTFLTIMEDR
                     EGSIWIGGDGLLRIR"
BASE COUNT          827 a         1367 c         1393 g          893 t
ORIGIN      
        1 cggaatctcc gcagtagcct tgcgcagggc gttggccacc tcctcgctgc tgcgcttgcg
       61 ctggtctttc gggaccaggc tgagacggat gtcggcgcta ttgccttccc ccacccgcgc
      121 catcatcgtg gtcatctccg gcacctcgcg gcgcacgatc tcctccacct gcagggcgat
      181 ccggtccacc acctccaggc gtgaaccgat ttcggcctcg aggctgacgt tcacctcacc
      241 ctcatcggtc tgcggcatca gctccacgcc gatgaaacgg accagcagca cggaaaaggc
      301 aaagagcccc agggcgagcc agatcaccag cttgcgccgg ccaagggcct tcctcagcag
      361 cccggaatag gcgcgctcca gcttttcaaa gagttcctgg ctcaccgtat agatcttctc
      421 gatgcggctg atctttttgc cgtgctgcac cgggtggtag cggaggaaac gtgaagagag
      481 catcggcacc agggtcagcg ccaccagcag ggagcagagc agggaaaaga ccacaacata
      541 ggccatctgc tgatacatga tgccggacat gccgcggatg aagattaccg gaaaaaagac
      601 gacgatggtg gtcagggtgc tggcgatgat ggcgccgctg acctctccgg cccccttgcg
      661 cgcagcctcg atcgctttga ggccgttttc ccggtagcga tagatatttt cgagcacaac
      721 gatagcgttg tcgaccagca ttccgatgcc gagggcgagc ccgccgaagg tgatgctgtt
      781 gagggtaaag ccgttgaaat acatcagccc gaaggtggcc acgatcgaga tcgggatggc
      841 cgtggcgatg atcgaggtgc tggagaggtt gcgcaggaag aggagcagga tgacgatcgc
      901 cagcaggccg ccgaagagag tgctgttggc gaccgagttg atcgactgct tgataaagac
      961 cgacgagtca aaattggggg tgatcttgat ctgcggcaga tcgaggttga tgcgttcaat
     1021 ctccttgttg acagcatccg caacctcaac cgtattggag cccgactgtt tgttgatggc
     1081 catgcgcagg cccgccttgc cgttgaggcg gttgagggag cgcacctcct gccaggaatc
     1141 ctcgacgttc gccacatcgc tgatccggat cggtgtgccc tggcgggtgg tgatgacggt
     1201 gttgcggatt tcctcgaggt tgacgtattc cccctgggtg cggatctgga tctcgaggtt
     1261 gcccttcttg aagagaccgg ccgggatggt ccggttctcg ctgcgcaggg cattgatgat
     1321 cgcattggtc gacagcccga gggccttgag ctgatccgcc ctgaggtcaa cgtggatctc
     1381 gcggtagagg ccgccccaga tgttgacggc ggccacaccc gggacgcgct cgacacggta
     1441 cttgacctgg ttttccacca gctggcgcag ctcgagggga tgcatgtcgc tggtgacgcc
     1501 catgaacatg atcggagtag cggagatatc aaacttgcgg atcatcggcc gttccatatc
     1561 ctcgggcagc cgcccgaggg cgcggtcgag acggtcgcgg atgtcgttgg aggcgacatc
     1621 caggtcggtg ccccaggcaa aggagcagcg cacgacactg cgcccctcgc tcgaggttga
     1681 ggtgatctcc tccaccccct gcacggctgc cagggtttcc tccaggggac gggtgatcag
     1741 ttcctccatc tcctgggggc cgacattgcc gtaatcgctc accaccgaga tggtcggata
     1801 ggtgatctcg ggcatgagat caatactgag gcgcgaaaag gagaccactc caaggacgat
     1861 gatgaccaga aagatgacgc tggtgagaat gggccggttg atgggaccgt tgacgaaagc
     1921 catgcgtgct gctcctgcca ggttggttta gttgcctgcc ggtgaggttc cgggcgctcc
     1981 gtttcccgct ccgggcagaa tgatcaggct gccctcctgc agcaggtgct gcccgagggt
     2041 gatcacccgc ccctccagcc tgggggagag gatctcggta cgttcaccgc tgacgatgcc
     2101 gatctcgacg ggaacatacc gcgcgctttt cttgtcggcg gagacgagga agagcccgct
     2161 cgccccctcg cggttgacga tcgccttgct cggcacggtc tgggcccggg cgctgctcct
     2221 cagggtgacc atggcgcggg cgaacatccc cggtttgagc aggcgttcgc cgttttccac
     2281 ctccacctcc attttggcca cgcgcgcggc ctcgtccaga accggtgcga tgcgcgccac
     2341 tgtgccctcc agtccgcgtc caggccaggt gtcgacatag accctggcgg cctggccgac
     2401 ctgaatgtgg ccatagtcgc gttcgacgat ggtggtgcgc acgatcaccc ggtcgatgcc
     2461 gaccaccgag accaccgctg aattgaccgt gagcagagcc ccttcgtcga cgaaacgttc
     2521 accgatgaaa ccgggttcgc tggcgttcag acgggtataa tcgagccgga tctgcgccga
     2581 cttgagggcg gcctggcgct gctccacctg ggcccgcgcc agctcgaggc gcgatttgcg
     2641 cgcgacctcg ttgctgctgg cgacatcgaa ttcggcctgg gcggcgatcc ccttctcttt
     2701 cagcagccgc accctctcga gctcctgtga agcgagcagc gcctggcttt cggcctcgat
     2761 cagggaggcc tcggcgatct tgaggttggc ctccgcctcg cgcagggctt gctggtattc
     2821 ggcgtcgtcg atgacagcga ccacctcacc ggcccgcacc cagtccccga tgcgtttgcg
     2881 gatggtcatc agccgtcccg aaaccttggg ggcgagtaca tattgataga tgggatagac
     2941 cgaaccggtg aactggcgga tctcctcgat cgcttcatgg cggacggtgt cggcctcaac
     3001 ggcgaccggc aattgcccgc cgccctgttt ggctttttcc ccaccgctga agatgagggt
     3061 gaaaatccgc cagaggagaa aaaggagaaa gagggctgca aggaggaaga ggatgcgtct
     3121 cttgttcata atctttcttt cgttggaatc tgcttgtgag ctgtataaaa aagacgcaca
     3181 taggcgtccc ttgttccgga aattatatct gtaattatac aaaaattatc ttttattggc
     3241 aagaattccc tcgcggggac gatcgccccc gccgcgatca gccatataaa aagtgttttg
     3301 aaaaatcctc catattctta tcttttagca tgcagatcgg gggatcccgc gcatgcctcc
     3361 ggcatgcccc cctgaccacc cgggtcgccg ggtgtgatct ccgctgagag aatggattcg
     3421 ctttgtgggg gcctatgcgc gcgttcagac tcaccctcct cctctttttc ctcctgctgc
     3481 aggccttcgg cgccgattca gcactggccc tcgatccggg caagctcatc acccagtacg
     3541 acatcaggaa ctacacggcc aaagatggcc tgccgatgaa tacggtcaag aaggtctttc
     3601 aggattcccg cggctacatc tgggtcggca cccaggaggg actggcgcgg ttcgacgggg
     3661 tgcgcttcag gatctacgac aagagcacgt cccccgggct gcgttcctcc ttcatcaggg
     3721 atatcgccga ggatcacgcc gggcatctct gggtcgccac caacggcggc ggcatcagcc
     3781 gcctcgaggg tgatgatttt gtcagctatg gcgccgccga gggcctgaac catgatgtcg
     3841 tcaacaccct gctcgtcgcc cgcgatggca gcctggtcat cggcaccgag aacggcattt
     3901 cccgctttgc ggacggacgg ctgacgacct ggatattcac agacaatgtg aaggccaata
     3961 atatccaggc cctgatccag gacagcaccg gtgtcctcta tgtgggacgc tatggtgctc
     4021 tgctgacccg catcgcctgg gctgatttca ccgcggcctc cggcactcca cagagtggtg
     4081 tcacctattg ccgcgtcccg ctcccggcac gtcgcaccga gatttctcct tttgaggaat
     4141 ctttcacggc cttgtgcacc acccgcgcgg gcgaccttct cgccggcaac accaatggaa
     4201 atgtcggatg ggtcgcaccg gcggccggcg gggctctcgc ccctttctgg tcgcccgact
     4261 cccctcgcga tcagataaca gtgcgggccc tgctcgagga ccgcgatggc ctcgtctgga
     4321 tctgcacgga ggggcggggg atcgtgcgct ggcatcaggg agagagcagc cggctcgatt
     4381 tggaacaggg tctggccacg agcggagaca ccttcttgac gatcatggag gaccgggagg
     4441 gatcgatctg gatcggcggc gacggtctgc tcaggatccg
//
LOCUS       DAOPWU010000290         4471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19554_length_4471_cov_4.645607, whole genome shotgun sequence.
ACCESSION   DAOPWU010000290 DAOPWU010000000
VERSION     DAOPWU010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19554_length_4471_cov_4.645607"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /gene="accC"
                     /locus_tag="PKM23_12355"
     CDS             complement(<1..1232)
                     /gene="accC"
                     /locus_tag="PKM23_12355"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNY92307.1"
                     /translation="MFKKILIANRGEIALRIIRACKELGIATVAVYSEADANSLHIRF
                     ADEAVCIGPAPSPKSYLNIPHLISAAEITNAEAIHPGYGFLAENAHFADICTSCGIVF
                     IGPTPEVISLMGDKARAKETMRSAGVPVIPGSDGTVHSVKAAAEVAARIGYPVIIKAV
                     AGGGGRGMRVVRFAHELANAYNTAQSEARAAFSNADLYIEKYFESPRHIEVQLLGDVY
                     GNVVALGERECSVQRKHQKLIEESPSPVVTPRLRREMSAAAVKGAKRVHYSSAGTIEF
                     LLDNRGHFYFMEMNTRIQVEHPVTEMVFGLDLVKEQIRLAAGARLRADLRFWKMRGHA
                     LECRINAEDPAHGFRPSPGTITSLHVPGGMGVRVDTHAYAQYLLPPNDGSLIAKLIVH
                     APTPEEAIIRMQRALDEF"
     gene            complement(1276..1758)
                     /gene="accB"
                     /locus_tag="PKM23_12360"
     CDS             complement(1276..1758)
                     /gene="accB"
                     /locus_tag="PKM23_12360"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNY92308.1"
                     /translation="MRAREIRELVKIVEESQIGELEITYWWGRKVRISKGIVGTGSGR
                     STGIEIVTAASQPAVTPLPHHTPAAMTPAPADNPGNLVEIKAPMVGTFYRAPAPEADP
                     YIREGDAVEPGKVLCIIEAMKLMNEIEAECSGRIVRILAQNGQPVEYNQPLFLVEPAS
                     "
     gene            complement(1786..2346)
                     /gene="efp"
                     /locus_tag="PKM23_12365"
     CDS             complement(1786..2346)
                     /gene="efp"
                     /locus_tag="PKM23_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNY92309.1"
                     /translation="MATTADFRTGYVIELEGELCAVLEFQHFKMGRGGSYVRTRLRNL
                     KTGRILEKTFRGGDKVDEAVLERRPMQYLYREGDSLLLMDNETYEQHSVPAAMLGSSV
                     GFLKESATVTVLLHRENPIGVEMPNFVELVVTSTEPGVRGDTVSGATKKAVLETGGTV
                     LVPLFIEEGEKLRIDTRTESYVERVR"
     gene            complement(2388..3896)
                     /gene="gatB"
                     /locus_tag="PKM23_12370"
     CDS             complement(2388..3896)
                     /gene="gatB"
                     /locus_tag="PKM23_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNY92310.1"
                     /translation="MNGTGASSRSKPGLVSAPFEVVIGLEVHIQLQTRSKIFCGCSVQ
                     FGAEANSQVCPVCLGLPGALPVLNEKVLASAARLALATGCRINPVSLFARKNYFYPDL
                     PKGYQISQFDQPFASDGGMTVTSEGDAQRAIRIRRIHLEDDAGRSIHAEAFVAEDETL
                     LDLNRCGVPLLELVTEPDLRSPAEAADFMQRLRQLVRYLGISSGNMEEGSLRCDANIS
                     LRPAGSDGLGVKTELKNMNSFRHVERALAFEIARQGALLAAGGRIEQQTLLWDPARGM
                     AEPMRSKEEAHDYRYFPEPDLMPVAISGEWVEELRRQMPELPEARKARYEREFGLPAY
                     DAGLLTGDPGVADYFERLAARIEDKKLASNWVMGEVMRASNARGCSVLELKMTPEHLA
                     VLLDSLSRGRIHQQAARRIFAEGLENGLSPAEAIEREAGAQIQDTDQLRMVVREALTS
                     HPDETEAWLGGKDKLAGFFTGVILQSLQGRADPRAIMALLQEERKALLEKRG"
     gene            complement(3893..>4471)
                     /locus_tag="PKM23_12375"
     CDS             complement(3893..>4471)
                     /locus_tag="PKM23_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNY92311.1"
                     /translation="PVSLPHSGYAIAAYYIIADAEASANLARFDGARYGRRSPAAAAL
                     EEMYTLSRSEGFGVEVKRRIMLGTFVLSSGYYEAYYGKAQKVRTLLQRDFAEAFRTCD
                     LLLTPVCPTTAFRIGEKAGDPLAMYLSDIFTVSVNMAGLPALALPCGRDSAGLPIGAQ
                     LIGPAFSEPLLLAAGHHLEKGHAPARAFAEAS"
BASE COUNT          794 a         1448 c         1387 g          842 t
ORIGIN      
        1 atgaactcat ccagcgcgcg ctgcatccga atgatcgcct cctccggggt cggggcgtgg
       61 acgatcagct tggcgatcag ggagccatca ttgggcggca gcagatactg ggcataggcg
      121 tgggtgtcga cgcgcacccc catcccgccc ggtacgtgca ggctggtgat cgtgcccggc
      181 gaagggcgga agccgtgggc cggatcttct gcattgatcc ggcactcgag ggcatggccg
      241 cgcatcttcc agaagcgcag atcggcgcgc agccgggccc cggcggcgag gcggatctgc
      301 tccttgacga gatctagccc gaaaaccatc tccgtgaccg gatgttcgac ctggatgcgg
      361 gtgttcatct ccatgaaata aaaatggcca cggttgtcga gcagaaactc gatcgtcccg
      421 gcgctgctgt aatgaacccg tttggccccc ttgacagcgg ccgcgctcat ctcgcggcga
      481 agccgcgggg tgaccacggg cgaggggctc tcctcgatca gcttctgatg cttgcgctgc
      541 accgagcatt cacgctcgcc cagggcgacg acattgccgt agacatcacc aagaagctgc
      601 acctcgatat ggcgaggcga ctcgaaatac ttttcgatat agagatcggc attgctgaag
      661 gcggccctgg cctcgctctg ggcggtatta taggcattgg ccagctcgtg ggcgaagcgc
      721 accacgcgca tgccgcgccc tccgccgccc gccaccgcct tgatgatgac cggatagccg
      781 atccgcgccg ccacctccgc tgccgccttg acgctgtgaa cggtgccgtc agagccggga
      841 attaccggca cccctgcgct gcgcatggtc tccttggcgc gggccttgtc ccccatcagg
      901 gagatcacct ccggggtcgg accgatgaaa acaattccgc aggaggtgca aatgtccgca
      961 aaatgggcat tctcggccag aaaaccgtag cccgggtgga tggcctcggc gttggtgatc
     1021 tcggcggcgc tgatgagatg gggaatgttc agatagcttt tggggctggg ggcgggaccg
     1081 atgcagaccg cctcgtccgc aaagcggatg tgcaggctgt tggcatccgc ctcgctgtat
     1141 accgccaccg tggcgatgcc aagttccttg caggcgcgga tgatgcgcag ggcgatctca
     1201 ccgcggttgg ctatgaggat tttcttgaac aaatagttcc ctgattaatg gtcagaaccg
     1261 gtcagacatc cccggtcagg aagcaggttc caccagaaat agaggctgat tgtattccac
     1321 aggctggccg ttctgggcca gtatcctgac gattctgccg ctgcactccg cctcgatctc
     1381 gttcatcagt ttcatcgcct cgatgatgca gagcaccttg ccgggctcga ccgcgtcgcc
     1441 ctcgcgtatg tagggatcag cctccggtgc gggcgcgcga taaaaagtcc ccaccatggg
     1501 cgccttgatc tcgaccaggt tgcccggatt atccgccggc gctggagtca tcgcggcagg
     1561 ggtgtgatgg ggcagcgggg ttaccgctgg ctgcgaagcc gcggtcacga tctcgatgcc
     1621 agtacttcgt ccgctgcccg tcccaacgat ccccttgctg atgcggacct tgcgccccca
     1681 ccagtaggtg atttccagct cgccgatctg gctctcttcc actattttaa ccagttccct
     1741 gatctctctg gcacgcatgc atactcctct tggttggtcg cggggctatc gcacccgttc
     1801 gacatagctc tccgtgcggg tatcgatcct gagcttctcg ccctcttcga taaagagggg
     1861 aacaagcacg gtgccgccgg tctcgaggac cgctttcttg gtcgcgccgg agacggtatc
     1921 cccgcgcacc ccgggctccg tcgaggtcac caccagctcg acgaaattgg gcatttcgac
     1981 gccgatggga ttttcgcgat ggagcaggac ggtcaccgtc gcgctctcct tcaaaaaacc
     2041 gacgctggag cccagcatcg ccgccggcac gctgtgctgc tcataggtct cgttgtccat
     2101 gagcagcagg ctgtcacctt cacgataaag atactgcatg ggccgccgct cgagcacggc
     2161 ctcatccacc ttgtcgccgc cgcggaaggt cttttcgagg atgcgtccgg tcttgaggtt
     2221 gcgcagtcgg gtgcggacat aggagccgcc gcgtcccatc ttgaagtgct ggaactcgag
     2281 cactgcgcag agttcaccct ccagttcgat cacatatccg gtcctgaaat cagctgttgt
     2341 agccatattt ttgtcgttca actccattca gaattcaatt caactgctca acccctcttt
     2401 tccagcaggg ccttgcgctc ttcctgcagc agggccatga tagcgcgggg atcggcgcgc
     2461 ccctgcaggc tctgcaggat caccccggtg aaaaagccgg ccagcttgtc ctttcccccc
     2521 agccaggcct cggtttcgtc gggatggctg gtcagggcct cccgtaccac catgcgcagc
     2581 tggtcagtgt cttggatctg ggcccccgcc tctcgttcga tcgcctcggc cggggaaagc
     2641 ccgttctcaa gcccctccgc gaagattctg cgcgccgcct gttgatggat ccggccccgg
     2701 ctcagcgaat ccaggagcac agcgaggtgt tccggggtca ttttgagctc caggactgag
     2761 cagccgcgcg cattgctggc acgcatcacc tcgcccatga cccagttgct ggccagtttt
     2821 ttatcctcga tgcgggcggc gagacgctcg aaatagtcgg ccacccccgg atctccggtc
     2881 agaagcccgg cgtcataagc cggcaggccg aactcacgct catagcgcgc cttgcgcgcc
     2941 tcggggagct cgggcatctg ccgtcgcagc tcctcgaccc actccccgct gatagccacc
     3001 ggcatgaggt cgggttcggg aaaataacgg taatcatggg cctcctcctt gctgcgcatg
     3061 ggctcggcca tgccccgcgc cgggtcccag agcagggtct gctgctcgat ccggcccccg
     3121 gcggccagca acgccccctg ccttgcgatc tcgaaggcga gggcgcgctc gacgtggcgg
     3181 aaggagttca tgttcttgag ctcggtcttg acccccaaac cgtcacttcc ggcgggccgg
     3241 agggagatat tggcatcgca gcgcaggctg ccctcctcca tattgccgct gctgatgcca
     3301 aggtagcgca cgagctggcg cagccgctgc ataaaatcgg cagcctcggc gggtgagcgc
     3361 agatcgggtt ccgtcaccag ctcgagcagg gggacgccgc agcggttgag atcgagcagg
     3421 gtttcatcct cggccacaaa ggcctcggca tggatcgacc ggcctgcatc atcttcaaga
     3481 tggatgcgcc ggatgcgaat agcccgctgc gcatcgccct cgcttgtcac ggtcatgccc
     3541 ccatcggagg caaagggctg atcaaactgg gagatctggt agcccttggg cagatccgga
     3601 taaaaatagt tcttgcgggc gaagagcgaa accgggttga tccggcagcc ggtggccagg
     3661 gcgagcctcg cggccgaggc aagcaccttt tcgttcagca ccggcagcgc tcccggcagc
     3721 cccaggcaga ccggacagac ctggctgttg gcctccgctc cgaactggac gctgcagccg
     3781 caaaagatct tgctcctggt ctgcagctgg atgtgcacct ccagcccgat caccacctca
     3841 aagggggcgc tgaccagacc gggcttgctg cggctgctcg ctcccgtccc gttcatgagg
     3901 cctctgcaaa ggcgcgggcc ggggcgtgac ccttttcgag gtggtggccg gcagccagca
     3961 gcagcggctc cgaaaatgcg gggccgatga gctgggcccc gatcgggagc ccggcgctgt
     4021 cgcggccgca gggcaaggcc agggccggca gccccgccat gttgaccgag acggtgaaga
     4081 tatcggagag atacatggca aggggatcgc ccgccttttc gccgatccgg aatgccgtgg
     4141 tcgggcaaac cggcgtcagc agcagatcgc aggtccgaaa ggcctcggca aaatcgcgct
     4201 gcagcagggt acgcaccttc tgggccttgc cgtaataagc ctcataatag ccgctcgaga
     4261 gcacaaaagt cccgagcatg atacgccgct tgacctccac gccaaaaccc tcgctgcgtg
     4321 acagggtgta catctcctcc aacgccgccg cggccgggct gcggcggcca tagcgagccc
     4381 cgtcgaaacg cgccagattg gcagaggcct cggcatcggc gatgatgtaa taggcggcga
     4441 tggcatagcc gctatgcggc agcgagaccg g
//
LOCUS       DAOPWU010000291         4469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19569_length_4469_cov_5.009968, whole genome shotgun sequence.
ACCESSION   DAOPWU010000291 DAOPWU010000000
VERSION     DAOPWU010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19569_length_4469_cov_5.009968"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PKM23_12380"
     CDS             <1..657
                     /locus_tag="PKM23_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY92312.1"
                     /translation="ERCHTCPEKVQCAFELDLEKNKNLKELYLDNEHYDGYFRDRCVF
                     RPGIDIEDTMNVLVRYDNQVTMAYSLNAFNAWEGYYVIFNGTKGRLEHKAEERVYLFG
                     DGTVPGALKGDGTYIRIYPLREPAYEVEVWTGEGGHGGGDAVMLADLFSPVRQADKYL
                     RAADQRSGAWSCLTGIAANRSIADGRPVAIADLADDIGYPDYPAMPGHEEPLPMPLKA
                     "
     gene            complement(647..2053)
                     /locus_tag="PKM23_12385"
     CDS             complement(647..2053)
                     /locus_tag="PKM23_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNY92313.1"
                     /translation="MNRKRLFPLLVVLALISTAGAQELTGSWQGALEVMGAKLRLVFN
                     FEQSDSGWTALLDSPDQGAKGIPCGQVRTPADSLIVEVPVVHGSYLALYSPDSLRYDG
                     VWRQGGLELPLQLVKSDQPVQMNRPQEPRPPFPYRSEEVTIPNKRAGLTLAGTLTLPE
                     GEGRHPAVILITGSGAQNRDEAIMGHRPFWVIADHLSRSGFAVLRCDDRGTGASTGDI
                     ASATSLDFAGDVAAQVDALVRRPDIDKKRIILAGHSEGGLIAPMVAAKNKKIAGIILL
                     AGPGVTGEKIMLAQGELIGRAAGQDEATVKQNRRIQEILFAIMKEGGEGLEEKLRAAM
                     AAQRSEMSASEQAAMTDQLINMQIKQLASPWFRFFLTHDPAPVLRKVKCPVLALNGEL
                     DLQVPADENLAAIASALHAGGNKRFETHKLYGLNHLFQTAKTGGVDEYSRIEETFSPR
                     ALELMTNWLARDFYTLMP"
     gene            complement(2147..>4469)
                     /locus_tag="PKM23_12390"
     CDS             complement(2147..>4469)
                     /locus_tag="PKM23_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92314.1"
                     /translation="DPAISESNSLRNNNIAAPPAGVFWRDFLLNHADEGYPLLRDQLK
                     GCKALFDGTRGKPEAKATALGRLNTWMEQTLAFDSKEERPHQPVRIYRVHMGRCGEWS
                     DLRNAAARAALIPCTSVASYSTDHVWNEFWDGQWYHWDNETNYPLMYYLSWGKVFGTV
                     FEIRSDGALTSVTNRYTRGICYIDLYVTDSAGRPVDGAEVNLYTKDPTSGELWWDMYA
                     LTDDKGQCTFVVGKDRSYYAAITSAIGSHPARAGSVVEIVANAVSGTDYSKKIKMTQT
                     KPAPKWTPAAPPADSGEDYYLEAEFSAPAALLNGAIRFDDVRLGSQLYQFDYDGSIDF
                     YFADSANYAAASAGSPFATVGLLPDSLEGTGWLSLPAGQPWHAVFGNEQNNRNVAQLS
                     ATVRLFARAAAINQQTLACSAGWNWIAFNVAPASTAVAELLAPLGSNGLRIRSAQGEA
                     VNEAGKGWQGSLQNLEAGKGYLLQLGSAQEFSVAAPLLPVEQPIPLEKGWYWIAYLAH
                     AGLPVAEALAPVADKLYQIKGQQHSAMYVEGRWIGDLERLEPGRMYKINMTAPAALSW
                     PNPQPVLARAAAAAEEVMTGTAGNMVVIARITSGHSFWKAIVVDDQGNTRGCGVPIPG
                     TGLYYFTIVGDVEKENLSVDLRVSPTGWQTRCVQKITFRDNATLGSLTAPLHLSDSAV
                     TFRLGQNYPNPFNSGTSLNYSLAEPGEARLVIYNALGRQVRVLLDGAQPAGEGTAAWD
                     GRDEYGRPAAAGIYFVQLSSGGSRQSMKMLLLH"
BASE COUNT          757 a         1332 c         1535 g          845 t
ORIGIN      
        1 gaacgctgcc atacctgccc cgaaaaggtc cagtgcgcct ttgaactcga cctcgagaaa
       61 aacaaaaacc tcaaggagct ctatctcgac aacgagcact acgatggcta tttccgtgac
      121 cgctgcgtct ttaggcccgg catcgacatc gaggacacca tgaacgtgct cgtgcgctac
      181 gacaaccagg tcaccatggc ctactccctc aacgccttca acgcctggga gggctattat
      241 gtgatcttta acgggacgaa agggcggctg gaacacaagg cggaggagag ggtctatctg
      301 ttcggggatg gcacggtgcc cggcgcgctc aagggggatg gcacctacat ccgtatctat
      361 cccctgcgcg agcccgctta tgaagttgag gtctggacgg gcgagggcgg ccacggcggc
      421 ggcgacgcgg tgatgctggc cgatctcttt tcgcctgtcc gtcaggccga caaatacctg
      481 cgcgcggccg accagcgcag cggggcctgg tcatgcctca ccggcatcgc cgccaatcgc
      541 tccatcgccg acggtcggcc ggtcgccatc gccgatctgg ccgatgatat tggctatccc
      601 gattatcccg ccatgccggg ccatgaggag cccctgccga tgcccctcaa ggcataagag
      661 tataaaaatc cctggccagc cagttggtca tcagctcaag cgcgcgcggt gaaaaagtct
      721 cctcgatgcg gctgtattcg tccacaccgc cggtctttgc ggtctggaag agatggttca
      781 ggccgtagag cttgtgggtc tcgaagcgtt tgttgccgcc ggcgtggagg gccgaggcaa
      841 tggcggcgag gttttcgtcc gccgggacct gcagatccag ttcgccgttg agggccagca
      901 ccgggcattt gaccttgcgc agcaccggcg cggggtcgtg ggtcaggaag aagcgaaacc
      961 agggcgaggc cagctgcttg atctgcatat tgatcaactg gtcggtcatg gcggcctgct
     1021 cggatgcgct catttcactg cgctgggcgg ccatggccgc gcgcagtttc tcttcgagtc
     1081 cctctcctcc ctccttcatg atcgcaaaga gaatctcctg aatacggcga ttctgcttca
     1141 cagtcgcttc atcctgacca gcggcgcggc cgatgagttc gccctgggcg agcatgatct
     1201 tttccccggt cacgccgggc ccggcgagaa ggatgatgcc ggcgatcttt ttgttctttg
     1261 cggcaaccat cggggcgatc agcccgccct cgctgtggcc ggcgaggata atgcgctttt
     1321 tgtcgatatc ggggcgccgg acgagggcgt cgacctgggc ggccacatcg ccggcaaaat
     1381 ccaggctggt ggcggacgcg atgtctccgg tcgaggcgcc cgtgccgcgg tcatcgcagc
     1441 gcagcaccgc aaagccgctg cggctgaggt ggtcggcgat gacccagaag ggccggtggc
     1501 ccatgatcgc ctcatcgcgg ttttgcgcgc ccgaaccggt gatgaggatg acagccggat
     1561 gacgcccctc cccttccggc agggtcaggg tgccggcaag ggtgaggcct gcacgcttgt
     1621 tggggatggt cacctcttcg ctgcggtaag ggaagggtgg cctgggctcc tgggggcggt
     1681 tcatctggac gggctggtcg cttttgacaa gctggagggg taattcgagt cccccctgcc
     1741 gccagacgcc atcgtagcgg aggctgtcgg ggctgtagag cgcaagataa gagccgtgga
     1801 cgaccgggac ctcgacgatg agagagtcgg cgggggtgcg gacctgaccg caggggatgc
     1861 ccttggcgcc ctggtccggg ctgtcgagca gggccgtcca gccgctgtca ctttgctcaa
     1921 agttgaagac gaggcggagt ttggcgccca tgacttccag ggcgccctgc cagctgccgg
     1981 tcagctcctg ggcgccggcg gtggagatga gtgccaggac caccaggagg gggaaaagcc
     2041 gttttctatt catctgattc tccggtttat gttgccaaga atacgctttc ccggagaaaa
     2101 tagcaagcaa aatttgctcc tgtccaccgg gagatggccg ccggcctcaa tgcagcagga
     2161 gcattttcat gctctggcgc gatcccccgg aagagagctg gacaaagtag atgcccgctg
     2221 cagcggggcg gccgtactca tccctgccgt cccaggcggc tgtgccctcg cccgccggct
     2281 gggctccatc caggagcacc cgcacctggc ggcccagggc gttgtagatc accagccggg
     2341 cctctcccgg ttcggcgagg ctgtagttca ggctcgtccc gctgttgaag ggattgggat
     2401 agttttgtcc gagccggaag gtgaccgcac tgtccgacag gtggagaggc gccgtcaggc
     2461 tgccgagggt ggcgttgtcc ctgaaggtga tcttttgtac gcatctggtc tgccaaccgg
     2521 tcgggctgac gcgcagatcg accgagaggt tttctttctc cacgtcgccg acgatggtga
     2581 agtagtagag gccggtcccg gggatgggca cgccgcagcc gcgcgtgttg ccctgatcgt
     2641 cgacgacgat cgccttccag aaggagtggc cgctggtgat gcgagcgatc acgaccatgt
     2701 taccggcggt tccagtcatg acctcctcgg ctgcggcggc ggccctggcg aggacgggct
     2761 ggggattggg ccagctcagg gcggccgggg cggtcatatt gatcttgtac atgcggccgg
     2821 gttcgaggcg ctcgaggtcg ccgatccatc tcccctccac atacatggcc gagtgctgct
     2881 gacccttgat ctgatagagc ttgtccgcga ccggggcgag ggcctcggcc accggcagcc
     2941 cggcgtgggc gagataggcg atccagtacc atcccttctc gagcgggatc ggctgctcaa
     3001 ccgggaggag cggcgccgcc acggagaact cctgcgccga accgagctgg agcagatagc
     3061 ccttgccagc ctccagattc tgcaggctgc cctgccagcc cttgcccgct tcattcaccg
     3121 cctcgccctg tgctgatctg atcctgagcc cgttgctgcc gaggggggcg agcagttcgg
     3181 ccaccgccgt cgaggcgggg gcgacgttaa aagcgatcca gttccagccg gcgctgcagg
     3241 ccagggtctg ctgattgatg gccgcggcgc gggcgaagag cctcactgtg gccgagagct
     3301 gtgccacatt gcggttgttc tgttcgttgc cgaaaacggc gtgccatggc tgccccgcgg
     3361 gcagcgagag ccagccggtt ccttcgagcg agtcgggcag cagcccgaca gtggcgaagg
     3421 ggctgccggc agaggcggct gcataattgg ccgagtcggc gaaatagaaa tcgatgctgc
     3481 cgtcgtaatc gaactggtag agctgggagc cgaggcggac gtcatcgaag cggatggcgc
     3541 cgttgaggag cgcagccggg gcgctgaact cggcctcgag ataatagtcc tcgccgctgt
     3601 cggccggggg tgctgcagga gtccatttcg gagccggttt ggtctgggtc atcttgatct
     3661 ttttgctgta atcggtgccg ctgaccgcgt tggcgacgat ctcaaccacg ctgcccgcgc
     3721 gggcgggatg gctgccgatc gccgaggtga tcgcggcata atagctgcgg tccttgccga
     3781 ccacgaaggt gcactggccc ttgtcatcgg tgagggcata catatcccac cacagctccc
     3841 cgctggtggg atctttggtg tagaggttga cttcggcgcc gtcgaccggc cggccggcgc
     3901 tgtcggtgac atagagatcg atatagcaga tgccgcgggt atagcggttg gtaaccgagg
     3961 tcagggcgcc gtcgctgcgg atctcaaaga ccgtgccgaa aaccttgccc cagctcaggt
     4021 agtacatgag agggtaattg gtctcgttat cccagtggta ccactggccg tcccagaatt
     4081 cattccagac atggtcggtg gagtaggagg cgacgctggt gcaggggatg agggcggcgc
     4141 gggcggcggc gttgcgcaga tcggaccact cgccgcagcg ccccatatgg acgcgataga
     4201 tgcgcaccgg ctggtggggg cgctcctcct tcgagtcgaa ggcgagggtc tgttccatcc
     4261 aggtgttgag gcgcccgagg gcagtggctt tggcctcggg tttgccgcgg gtgccgtcga
     4321 agagggcctt gcagcccttg agctgatcgc ggagaagggg atagccctcg tcggcgtggt
     4381 tgaggagaaa atcgcgccag aaaaccccgg cgggcggggc cgcaatgttg ttgttgcgca
     4441 gggagttgct ctcggagata gcggggtcg
//
LOCUS       DAOPWU010000292         4457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19655_length_4457_cov_5.247842, whole genome shotgun sequence.
ACCESSION   DAOPWU010000292 DAOPWU010000000
VERSION     DAOPWU010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19655_length_4457_cov_5.247842"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1056)
                     /locus_tag="PKM23_12395"
     CDS             complement(112..1056)
                     /locus_tag="PKM23_12395"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNY92315.1"
                     /translation="MDIFIQAADIVAPVFIIVFIGLALRRAGMIDAHFNALSSRLVFM
                     VTLPALLFAKISSTRIQELIDGRQILFAYLFIFISFAIAWLAGLRLAKSGRDRGAFIQ
                     GAFRGNFAILGFAMLNNAYGPGVLAPAAVVLAAIMPPYNILAVLALSLTQKRERDISG
                     RAILKQILTNPLILAAAGAVPFSLFQIRLPQMLLTSIDHLSSLTLPLALIGIGGSLSF
                     KGVREDFRLAFHATLLKIVLLPILVIAAAVHAGFHGPELGVLFFFFASPTAIASYAMA
                     EAMGSNSRLAGHIILLTTMGSIITISTGIIILKSMGVF"
     gene            complement(1077..2468)
                     /locus_tag="PKM23_12400"
     CDS             complement(1077..2468)
                     /locus_tag="PKM23_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY92316.1"
                     /translation="MPERALQRAALAVAMLTSFVTPFMGSAVNIALPGIGAELGMDAV
                     MLTWIASAYILAAVVFLLPFGRLADIHGRKKVFSIGILFFLLGSLAAALSRSGGMLIA
                     ARVIQGLGGGLVFGTATAILISVYPPERRGRVIGWNIASVYIGLSLGPPAGGWMVGHF
                     GWRSIFWLNSLLCAVSLGLTLRYLRREWKEAAGEKFDAPGAAAYALSIVALMYGLSSL
                     PARRGYAALAAAAAALFLFIRREGRTRFPLLEVRLLRGNRVFAFSNLAALISYSATFA
                     VGFLLSLYLQYVKGFSPQQAGLVMISQPVMQALFSPVAGRLSDRIEPRLIASAGMALT
                     AAGLALLFFLGPTTGVAYISLSLVVLGIGFGLFSSPNTNAIMGSVERRHYSVASATLA
                     TMRMLGQMFSMGMTALVIALFVGHRPIGAENQGGFLGGVHLLLGGFALLSLAGIFASL
                     ARGRVHKGVGAAD"
     gene            complement(2468..3049)
                     /locus_tag="PKM23_12405"
     CDS             complement(2468..3049)
                     /locus_tag="PKM23_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNY92317.1"
                     /translation="MSESMRVPEAEVDPQFPERWSPRAFSPRPVAEADLLSLFEAARW
                     APSAANSQPWFFLAAVTPEERARFLALLNPGNQVWAREAPVLLFVLTRRRRASGRPME
                     WGAFDAGAAWMSLALQARKLGLFAHAMGGFDREGIYAALNIPVEEYLALAAVAVGYYG
                     DPALLSEEDQQREHPSLRKPLRELFHYGALGEE"
     gene            3445..3873
                     /locus_tag="PKM23_12410"
     CDS             3445..3873
                     /locus_tag="PKM23_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNY92318.1"
                     /translation="MLLARRDRDNLVRNPWFDEAFTGWPFWSRGEEDSMVWSPRVDVR
                     ENENEVIVKADLPGMDKKDLKIAVENNLLSIKGERKAEFEEKDADCYRSERLFGTFQR
                     TFSLPSRIKADAIRAEYKDGVLTVVLPKVEEVKPRQIEIS"
     gene            complement(4001..4435)
                     /locus_tag="PKM23_12415"
     CDS             complement(4001..4435)
                     /locus_tag="PKM23_12415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNY92319.1"
                     /translation="MFCIFCEIAAKRIPAQIVYEDGEVIAFMDLMPITKGHVLVVPKV
                     HAENLYELTPAAAAAMIPAAQKIAAALRASGLPCEGLNLQMANGKVAGQSVFHAHLHI
                     IPRNAGDGFGLRFPPGYGREADKEELTATAERIRTALAAKPA"
BASE COUNT          913 a         1310 c         1526 g          708 t
ORIGIN      
        1 aagtatgtcg agatcctcgg cggcctcatc ctcatcggca tcggccttaa aatcctcctc
       61 gaacatctcc agctgcttcc ctgatccttt ccataaaccc ctgcttgcgg attaaaagac
      121 ccccatcgac tttaggataa tgatgccggt cgagatcgtg atgatgctcc ccatcgtggt
      181 caggaggatg atgtggccgg ccaggcggct gttgctgccc atggcctcgg ccatggcata
      241 actggcgatg gcggtgggcg aggcgaagaa aaagaagagc acgcccagtt ccggaccgtg
      301 aaagcccgca tggaccgcgg cggcgatgac gaggatgggc aggaggacga tcttgagcag
      361 ggtggcgtga aaagcgagcc ggaaatcctc ccgcaccccc ttgaagctca gggatccgcc
      421 gatgccgatg agggcgaggg gcagggtgag ggaggagaga tggtcgatgc tggtcagaag
      481 catctgcggc agccggatct ggaagaggga gaaggggacg gccccggcgg cggcgaggat
      541 gaggggattg gtgaggatct gtttaaggat cgcccggccg ctgatgtcgc gttccctctt
      601 ctgggtcagc gagagcgcca gtacggcgag gatgttatag gggggcataa tcgcggccag
      661 gaccacggcc gccggagcga gcacccccgg cccgtaggcg ttgttgagca tggcgaagcc
      721 aaggatggcg aaattgccgc gaaaggcccc ctggataaag gccccgcggt cgcgcccgct
      781 tttcgccagg cgcaggccgg ccagccaggc gatggcgaag gagatgaaga tgaagaggta
      841 ggcgaaaagg atctggcgcc cgtcgatcag ctcctggatg cgcgtggagg agattttggc
      901 gaagagcagg gcgggcaggg tgaccatgaa gaccagccgg gaggagaggg cgttgaaatg
      961 ggcgtcgatc atgccggctc ggcgcagggc caggccgatg aagacgatga tgaagaccgg
     1021 ggcgacaatg tccgccgcct gaataaagat gtccattaca gctgctcctg aatccgttag
     1081 tctgctgcac ccacgccctt gtgcacccgg ccccgcgcca aggaggcgaa gatgccggcc
     1141 agggagagga gggcgaaccc gcccagcagc agatgcacgc ccccgagaaa gcccccctgg
     1201 ttctcagcac cgatcggccg gtggccgacg aagagggcga tgaccagagc ggtcataccc
     1261 atgctgaaca tctggccgag catgcgcatg gtggcgaggg tggcggaggc gacgctgtag
     1321 tggcgccgtt ccaccgagcc catgatggcg ttggtattgg gggaagagaa gaggccgaag
     1381 ccgatgccca gcaccaccag gctgaggctg atataggcca ccccggtggt ggggccaagg
     1441 aagaagagca gcgccagtcc cgccgccgtc agggccattc cggccgaggc gatcagacgc
     1501 ggctcgatgc ggtcggagag gcggccggcc accggcgaaa agagggcctg catcaccggc
     1561 tgggagatca tcaccagccc cgcctgctgg ggagagaatc ccttgacgta ctgcagatag
     1621 aggctgagca gaaaaccgac cgcgaaagtg gcgctgtagc tgatgagggc ggcgagattg
     1681 gaaaaggcga agacccggtt gccgcgcagc agacgcacct ccaggagggg aaagcgggtg
     1741 cgtccctccc ggcggatgaa gagaaagagg gcggccgcgg cggcggcgag ggcggcatag
     1801 ccgcgtcgcg ccggcaggct ggagaggccg tacatcaggg cgacaatgct cagggcgtag
     1861 gcggcggcgc cgggggcgtc aaatttttct ccggccgcct ccttccattc ccggcgcaga
     1921 taacggaggg tcagcccgag cgaaacggcg cagagaaggc tgttgagcca gaagatgctg
     1981 cgccagccga agtggccgac catccagccg ccggcgggcg ggccgaggga gaggccgatg
     2041 taaaccgagg cgatgttcca gccgatcacc cggccgcgcc gctccggggg atagaccgag
     2101 atcaggatgg ccgtggcggt gccgaaaacc agtccgcccc ccagcccctg gatgacgcgg
     2161 gcggcgatga gcatgccgcc gctgcgggag agggccgcgg ccagggagcc gaggaggaag
     2221 aaaaggatgc cgatgctgaa gacctttttg cggccatgaa tgtccgcgag ccgcccgaag
     2281 gggaggagaa agaccaccgc tgccaggata taggcggagg cgatccaggt cagcatcacc
     2341 gcatccatcc ccagctccgc gccgattccg ggcagggcga tgttgactgc agaacccatg
     2401 aagggggtga cgaaagaggt gagcatcgcg accgccaggg ccgcgcgctg cagggcgcgc
     2461 tccggcatca ctcctccccc agtgcgccat aatgaaagag ctcccggagc ggcttgcgca
     2521 gcgagggatg ctcgcgctgc tgatcctcct cgctgaggag ggcgggatcc ccgtagtatc
     2581 ccaccgccac cgcggccagg gcgagatact cctcgaccgg gatgttgagg gcggcataga
     2641 tcccctcgcg gtcaaagccc cccatggcgt gggcgaaaag gccaagcttg cgcgcctgca
     2701 gggcgagcga catccaggcc gccccggcat cgaaggcgcc ccactccatg ggacgtccgc
     2761 tggcgcggcg ccgccgcgtc agcacaaaga gcagcaccgg ggcctcgcgg gcccagacct
     2821 ggttgccggg attgagcagc gcgagaaaac gggcgcgctc ctccggggtc accgccgcca
     2881 gaaagaacca gggctgggag ttggcggccg agggggccca gcgcgccgcc tcgaagagcg
     2941 aaagcagatc ggcctccgcc accggccgcg gggaaaaagc ccgcggcgac caacgttcag
     3001 ggaactgggg atcgacctcc gcctcaggca cacgcatcga ttcagacatg ctcttctccg
     3061 cttcaggttc tccggccgct cgcactctgc tcagggtccg cgcctcactc tctgcgcgct
     3121 ggcgaatcgc cccgcccggt tttgaccgtc aatataccat tttcgcatct ataactcaat
     3181 gctttttatt ggaccggccg cattctgccg ggccgcccgc ctgattagtc tgccaatttg
     3241 acaaatacga cctcaggaac cgacaagatg acagatgttc tgccgctatg gcacaaccgt
     3301 tctcttttgg cgtggtatgg cacaaaacta atataaacag tacttaatga tataaataag
     3361 aatctggcac gctctttgta atgtaccggt gcgtaagcgt cacagctcaa ataggcaagt
     3421 ctaatcatac aaaaggaggt tcatatgttg ctggcaagac gtgatcgtga taatctggtg
     3481 cgcaatccct ggttcgatga ggcctttacg ggttggccct tctggagccg cggggaagag
     3541 gattcgatgg tctggtcgcc gcgtgtcgat gtgcgcgaga acgaaaatga ggtcatcgtc
     3601 aaggccgatc ttcccggcat ggacaagaaa gatctcaaga tcgcggtgga gaacaatctg
     3661 ctctccatca agggcgagcg caaggccgag ttcgaagaga aagatgccga ctgctatcgc
     3721 tccgagcggc tcttcggcac ttttcagcgc actttcagcc tgcccagccg gatcaaggcc
     3781 gatgccatcc gggccgagta caaggatggt gttctgaccg tcgtcctgcc caaggtcgaa
     3841 gaggtcaaac cgcgccagat cgaaatcagc tgaaaccagt ctgaaacgga aaagccccgc
     3901 tcccgcaagg gagtggggct ttttttatat cggagcggcg gtggcgcctg ccggcgggca
     3961 tgacaaccgg ttttccaggc tgccgcagcc ggcgccggcc tcaggcgggt ttggcggcca
     4021 gggccgtgcg gatacgctcc gcagtggcgg tgagctcctc cttgtcggcc tcccggccgt
     4081 agccgggggg gaatctcaga ccgaatccgt ccccggcgtt gcgggggatg atgtggagat
     4141 gggcgtgaaa gaccgactgt cccgcgacct tgccgttggc catttgcaga ttcagcccct
     4201 cgcaggggag gccggaggcg cgcagggcgg cggcgatctt ttgcgccgcg gggatcatcg
     4261 ccgcggcggc cgccggcgtc agctcataga ggttttcggc gtggactttg gggacgacca
     4321 gcacatgtcc tttggtgatg ggcatcaggt ccatgaaggc gatgacctcc ccgtcttcat
     4381 agacgatctg ggccgggatc cgtttggcgg cgatttcgca gaatatgcag aacatgtttc
     4441 ttcctcgctt tcagtag
//
LOCUS       DAOPWU010000293         4415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_19929_length_4415_cov_4.211468, whole genome shotgun sequence.
ACCESSION   DAOPWU010000293 DAOPWU010000000
VERSION     DAOPWU010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19929_length_4415_cov_4.211468"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKM23_12420"
     CDS             <1..283
                     /locus_tag="PKM23_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNY92320.1"
                     /translation="RAFGHFKIQLVDFKLEYGVIDGVVHLIDEITGGSFRLWPYAKEN
                     PDLSLPNLLSELDPAGRLDKDTYRMGGATDKVLAKFQQIAAITARFREI"
     gene            286..2610
                     /locus_tag="PKM23_12425"
     CDS             286..2610
                     /locus_tag="PKM23_12425"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNY92321.1"
                     /translation="MTGCRPLVLLLLTILLFPPLPAGALNPADSPAPCFSGAKAFANV
                     EELCKPEYGGRFTGHPGATRAAEWIGRQFENWGLVPAGDDRGWLQFYPMIVTEQLERA
                     KLTLKNGAFGPVDYQEGNDFTVYMNSGSGKVTAELLFAGFGISEPAMGWDDYAGVDAA
                     GKIVLIQRGLPADGQDWSFANERDYKMRTAAAHGAVGLLMLERDDWPVRGGTIHEEGY
                     QPDLPALNVSKKLVRDLFHGTFKDPDLILRDLPKKPNSLVLDRTLQLQVRVRRIEPGR
                     GENVVALLPGSDPLLREEYLVIGGHMDHCGVGRDGHIYAGADDNASGTAVVMELARLF
                     AARPERPKRSLLFIAFGGEEQGLRGSRYFAHNPPVPARQIAAMINFDMEGSGDGGARL
                     GGRNYFPGLVRSMVGSWSDSVLAKTGFGRGWGGSGSDHFHFIEQGISAFYFSSSGSHP
                     FYHQFEDQPRTLNTASLQSVGDRAEALIMALANQAEPLTLDNAGRGRFFLRHGDQLDL
                     CGCTAALKGDLAGAVAVAHDAGIHFTTLTLDGGEEGKAELFSRLDSLEQWIAAHPQHL
                     ARYENGGTIDRAAGQGKLAVGIRLKGTRALRGEAAVMRQLGRLGLNILTIDSPEDPLW
                     AEIGNGKAGKALLRAAETASMLLHVDLADTSLAGALLTAWKGRAVWSLDLAAAAVMPG
                     AVARRLADGKTMALLRCRPEDDPGGLLELAAQLPSDRIHISVLGSPESRELKRMEGFV
                     STLHSLAAAKMGREESYKQMEKWCGRNIRRILQP"
     gene            2731..3945
                     /locus_tag="PKM23_12430"
     CDS             2731..3945
                     /locus_tag="PKM23_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNY92322.1"
                     /translation="MSDSLALIKGSEFARHWRLRADIAYLNHGSFGACPKAVLEVQDS
                     FRWKLESSPWRFQVIEMAGHHAAARTALAQFLHASPDDIVFVPNATHGVNTVLRSLSF
                     RRGDELLTTNQDYFACRNAMEFAARRDGARLVVAKLPFPLEFPEQIIDAFLAAVTPRT
                     RLALIDHVTSPTGLVMPVQELVYELGRRNIITLVDGAHAPGMVPLNLHEIDADYFTGN
                     CHKWLCAPKGAAFLHVRRDLQEQIHPLAISHVAGEVAPPLNPFQHEFFWAGTRDTSAY
                     LAVASALKFMGHLVEGGWPEVMRRNRQLAVDARQLLCEALHIKPPCPDEMVGAMAALP
                     IPPAAALPDPLPPYFVDPLQEELFQRWQIDVPVVYFPRPPERLLRISAQIYNSLSQYE
                     RLAEVLRDRAGR"
     gene            complement(3923..>4415)
                     /locus_tag="PKM23_12435"
     CDS             complement(3923..>4415)
                     /locus_tag="PKM23_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNY92323.1"
                     /translation="ELVMKTLYLLRHGKADRPAGMLSDKERPLLKHGVEETAKICHKM
                     AKKGMFPEAIIASTAVRALQTAETAAQELGYESERLEKAEVIYGAEADELGLFLRHQS
                     DARSSLMLVGHNPGLEELAGMLGNEDAGHLPTGGVIVIRLAIESWRETAPGCGRITAR
                     LYP"
BASE COUNT          761 a         1461 c         1344 g          849 t
ORIGIN      
        1 gcgggccttt ggccatttca aaatccagct ggtcgatttc aagctcgaat acggagtgat
       61 cgacggcgtg gtccatctca tcgacgagat caccggcggt tccttccgcc tctggcccta
      121 tgccaaggag aatcccgatc tcagcctgcc caatctgctc tcagaactcg atccggcggg
      181 ccggctggac aaggacacct accgaatggg cggggccacc gacaaggtgt tggccaaatt
      241 ccagcagatt gccgccatta ctgcccgttt cagggagatc tgaccatgac cggctgccgc
      301 cccctcgtgc tccttctgct caccattttg ctctttccac cccttcctgc cggggccttg
      361 aatcccgcgg attccccggc gccgtgtttc tccggcgcca aagccttcgc caatgtcgag
      421 gagctgtgta aaccggaata cggggggcgc ttcaccggcc atcccggtgc cacccgggcg
      481 gccgagtgga tcggaaggca gtttgagaac tgggggctgg tcccggcggg ggatgaccgc
      541 ggctggctgc agttctatcc gatgatagtc accgaacagc tggagcgggc gaaactgacg
      601 ctcaaaaacg gggccttcgg cccggtcgac tatcaggagg gcaacgattt caccgtctac
      661 atgaactcgg gttcgggcaa ggtgacggcg gagctcctct tcgccgggtt tggcatcagc
      721 gaaccggcca tggggtggga cgactatgcc ggcgtcgatg ccgcgggcaa gattgtgctg
      781 atccagcgcg gcctgcccgc tgacgggcag gattggagct tcgccaacga gcgggattac
      841 aagatgcgca ccgctgccgc ccacggtgct gtggggctcc tgatgctcga gcgcgatgat
      901 tggcctgtgc gcggaggcac catccatgaa gagggttatc agcccgatct gcccgcgctc
      961 aatgtctcaa aaaagctggt ccgggacctc tttcacggca ccttcaagga tcccgacctc
     1021 atcctgcgcg atctgcctaa aaagcccaac tccctggttc ttgaccgcac cctgcagctg
     1081 caggtgcggg tgcggcggat cgaacccggg cggggcgaga atgtcgtcgc cctgctcccc
     1141 ggttctgatc cccttttgcg cgaggagtat ctggtcatcg gcggccacat ggatcactgc
     1201 ggcgtcggcc gggacggcca catctatgcc ggcgctgatg acaacgcctc gggcacagcc
     1261 gtggtcatgg aactggcgcg ccttttcgcc gcccgtcccg aacgtccgaa acgatcgctg
     1321 ctctttattg ccttcggggg agaggaacag ggattgcgcg gctccagata tttcgcccat
     1381 aatccgccgg tgccggcccg gcaaatcgct gcgatgatca attttgacat ggaggggagc
     1441 ggtgacggcg gggcgcgcct gggcggcagg aactatttcc cggggttggt ccggtcgatg
     1501 gtaggctcgt ggagcgattc cgtgctcgcc aaaaccggct ttggccgcgg ctggggcggg
     1561 tccggttccg accatttcca tttcatcgag caggggatat ccgcctttta tttcagctcc
     1621 tcaggcagcc atccctttta ccaccagttt gaggatcagc cccgcaccct caatacggcc
     1681 tccctgcaaa gcgtcggcga ccgcgcggag gccctgatca tggccctggc gaaccaggcc
     1741 gaacccctca ctcttgacaa tgccggccgc gggcgcttct tccttcgcca tggcgaccag
     1801 ctcgatctat gcggatgcac tgcagccctg aagggcgatc tggccggagc ggtggctgtc
     1861 gctcatgacg ccggcatcca tttcaccacc ctgactctcg acggagggga ggagggaaag
     1921 gcggagcttt tcagccgcct cgacagcctc gaacagtgga tcgccgccca cccgcagcat
     1981 ctcgcccgct atgaaaacgg cggcaccatc gaccgtgccg ccggccaggg caagctggcc
     2041 gtcggcatcc gcctgaaagg caccagggct ctgcgcggcg aggctgcggt catgcgccag
     2101 cttggccggc tcggcctcaa cattttgacc atcgattcgc ccgaggatcc gctgtgggcc
     2161 gagatcggga acgggaaggc cggtaaggca ctcctgcgcg ccgctgaaac cgcctccatg
     2221 ctcctgcatg tcgatctcgc cgataccagc ctcgccgggg ccctcctcac agcctggaaa
     2281 ggccgggcgg tctggtcgct tgaccttgcc gcagccgcag tcatgcccgg tgctgtcgcc
     2341 aggcggctgg cggacggaaa gacgatggcg ctcctgcgct gccggccgga agatgatccc
     2401 ggcgggctgc tcgaactggc cgcgcaactg ccctccgacc ggattcatat ctcggttctc
     2461 ggcagtccgg aatcccggga gctgaaaagg atggagggtt ttgtcagcac cctgcactcc
     2521 ctggcggccg ccaaaatggg cagggaggag agctataaac agatggaaaa atggtgcggc
     2581 aggaacatca ggcgaatcct ccagccctga ccggctattc aggagagtgg gggagatcat
     2641 ggtcgggagc cggagccgaa cagagtaaac cagcatgtgc cagagcctaa cacggccggt
     2701 ggggaccggg agcaggaagg aaaatgagga atgagtgaca gcctggcctt gatcaaggga
     2761 tccgagtttg cacgccactg gcgtctgcgc gcggatatcg cctatctcaa tcatggctcc
     2821 ttcggcgcct gccccaaggc ggtgcttgag gtgcaggaca gtttccgctg gaagctcgaa
     2881 tccagtccct ggcgtttcca ggtcattgag atggccggtc atcatgccgc cgcccgcacc
     2941 gccctggcgc agttccttca cgcctccccg gacgatatcg tcttcgtccc caatgcgact
     3001 cacggcgtca ataccgtttt gcgctcgctc tccttcagga ggggggatga gctgctgacc
     3061 accaatcagg actattttgc ctgccgcaac gccatggaat tcgccgcgcg ccgcgatggc
     3121 gcgcgcctgg tggtggcgaa actgcctttc cctctcgaat ttcccgaaca gatcatcgat
     3181 gcttttctcg cggccgtgac cccgcggacg cggctggccc tcatcgatca tgtcaccagt
     3241 ccgaccggcc tggtcatgcc ggtccaggag ctggtttacg aactcggccg ccgcaatatc
     3301 atcaccctgg ttgacggcgc ccacgccccc ggcatggtgc cgctcaatct gcacgagatc
     3361 gatgccgatt acttcaccgg gaactgccac aagtggctct gcgcccccaa gggcgcggcc
     3421 tttctgcatg tccgccgcga cctgcaggag cagattcatc ccctcgccat cagccatgtc
     3481 gccggggagg tcgcccctcc cctcaatccc ttccagcatg aattcttttg ggcgggtacg
     3541 cgcgatacca gtgcctacct cgctgtcgcc tccgcgctca aattcatggg tcatctggtc
     3601 gaagggggat ggccggaggt catgcgccgc aaccgccagc tcgccgtgga cgcccggcag
     3661 cttctctgcg aggccctgca catcaagcct ccctgtccgg acgagatggt cggcgccatg
     3721 gctgcactgc ccataccccc cgcagcggcc ctcccggatc ccctgccgcc ctattttgtc
     3781 gatcccctgc aggaggaact cttccagaga tggcagatcg acgtcccggt tgtctatttt
     3841 ccccgcccgc cggagcgtct gctgcgcatc tcggcgcaga tctacaacag cctttcccaa
     3901 tatgagcgcc tcgccgaggt gctcagggat agagccgggc ggtgatcctg ccgcagccgg
     3961 gggccgtttc acgccagctt tcaatggcga ggcggatgac gatcacccct ccggtcggga
     4021 ggtgtccggc atcttcgttc cccagcatcc cggccaactc ctcaagtccc ggattatgac
     4081 ccaccagcat cagcgacgag cgcgcgtcgc tctgatgacg caaaaagagg cccagctcgt
     4141 cggcctcggc gccatagatc acctctgctt tttcgagccg ctcggactcg taccccagct
     4201 cctgcgccgc tgtttccgct gtctgcaggg cccgcactgc tgtgctggcg atgatcgcct
     4261 cgggaaacat tccctttttg gccattttat ggcagatttt ggctgtctct tcgacgccat
     4321 gtttgagaag gggccgctcc ttgtccgaga gcatgccggc cggccggtcg gccttgccgt
     4381 gccgcagcag atagagggtt ttcatgacca gctcc
//
LOCUS       DAOPWU010000294         4396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_20044_length_4396_cov_5.903939, whole genome shotgun sequence.
ACCESSION   DAOPWU010000294 DAOPWU010000000
VERSION     DAOPWU010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20044_length_4396_cov_5.903939"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKM23_12440"
     CDS             <1..419
                     /locus_tag="PKM23_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92324.1"
                     /translation="LARFESGEPAITSAGYGRGRAILIGSYIGLAYHRSKLAANGELI
                     ASLVEMAAPLDRPHLTSGRDIRIDLMRNGEGEVMLILQNLRHNEAEAMVALPEPVAGP
                     LREQFSGERLLFTAQGGGVQAAVHFAPNEVKVYCGR"
     gene            481..1692
                     /locus_tag="PKM23_12445"
     CDS             481..1692
                     /locus_tag="PKM23_12445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY92325.1"
                     /translation="MMDGALYAFGLSFASQQTVLPVFVQKIGGGNVAIGLIPVLWMLS
                     FNLPQILIASHAQRQRHKKRLLLKTAMGQRLPWLLLAVLAFFILPRVTPLAGLMLFFI
                     IYASAGIGGSINLPVWFDIFAKMTPISLRGRLFAARMILGGVLGIGAGIAVRHILDWL
                     PYPGNFGLLFLLTFAAMLVSYFFLTILKEPRSARSRTAAPLLREYLGALPSIIREERN
                     YRNFLIADGALYLAWMANGFFTVHALSRFDLPASAAGTFTAIMMASNIAGNLFFGWLA
                     DHFGQRINLILSAGMMTLSCALAIFAPGPELYCLVFVGSALAIALVQISRLPLIAELC
                     SAEQRPTFVALANLISSPFIIAGVAGGWLADRCGYEFLFACSGLFALFSMGWYASVVR
                     EPRGTAHERVF"
     gene            1707..3074
                     /locus_tag="PKM23_12450"
     CDS             1707..3074
                     /locus_tag="PKM23_12450"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNY92326.1"
                     /translation="MTRTFLLFTLLSCAVPLLAAQLQEAPAPLPPDPRFKSDILLIVA
                     HPDDETAIGSWLARAVYDLHKRVAILYLNRGEGGGNSFGNEQSSAMGAIREIEVRRAA
                     AAFGIDLLWFLDGQDTPGQDLMHSLTNWGHGENLERIVRLIRLTRPEMIITWLPHYGA
                     GENHGDHQASGILAVEAFDMAGDPTRFPAQIVPPRERADINNFTEGLQPWQPKKLYFF
                     SDVSHPLPSTGPAFNVHEISPSQKVPYYELAARLHLPHLTQGDVAETALAAQESGDYS
                     AFAEWISGFKLIFGKAVVPCAPSGDLFEGIKEGPLPFVQVRGYQPHPNAGIALELGGL
                     FAFYRDFWRAHEIEAIEILLKPEVMINAGGYLHIPLLLINNTSESVQVELRAQIPEGW
                     RNVAGEALYTLPAGAVRPVQTFFFAPERTNPSGETILWNAKVGERTAGSVSMQVFLAE
                     WTLPQ"
     gene            3108..>4396
                     /locus_tag="PKM23_12455"
     CDS             3108..>4396
                     /locus_tag="PKM23_12455"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNY92327.1"
                     /translation="MKRLRLNSALRWWLCLLLPAAVAACSCGCGARERGEEDYARLVN
                     PFVGTAGGGNTFPGAVRPWGMVSTSPHTAPSAPSGYYHGQPFNYGFGMVHLSGTGCAD
                     LGSVIVAVSRGEIKTRPEEYRSAFSEEQAEPAYYSCLLQEPAVRVRTSATERCGIFEF
                     VPLQDGEINIQIDAGRNLSRLGGGAIRLTRAGGIEGFNIGGGFCGENNRHQIFFSGEV
                     NLQPADAGVWRGEELLTANEANTEEEAVGGWLRIALRAGQPLMLRLGISYVSIANARE
                     NLRVEIPHWDFERVRRDGREAWQEQLSAVHIREGSEKERIKFYTALYHSLIHPGIISD
                     VNGEYPLMGRSGTGCNRERARYSVFSLWDTYRTLHPLLTLLYPSRQSEIIKTMLDMYQ
                     ESGFLPKWELAGNETYMMVGDGAVPVIADSYIKGIED"
BASE COUNT          786 a         1376 c         1275 g          959 t
ORIGIN      
        1 tcctcgcccg cttcgaaagc ggcgaacccg ccatcacctc agccggttac ggccgcggca
       61 gagcaatcct catcggttcc tacatcggcc tcgcttatca tcgcagcaag ctggccgcca
      121 atggagagct gatcgccagt ctggtcgaga tggccgctcc gctggaccgt ccacacctga
      181 caagcggccg tgacatccgc atcgacctga tgcggaatgg cgaaggcgag gtcatgctca
      241 tcctccagaa tctgcgccac aacgaggccg aggctatggt ggcacttccg gagccggtgg
      301 ccgggccgct ccgcgagcag ttctccggtg aacggctgct tttcacggcg caaggcgggg
      361 gtgtacaggc cgccgtccat ttcgcgccca atgaggtcaa agtctattgt ggccgctgaa
      421 cgggactctt tttttgcggg atccggcggc cgggttctga ggcataactt tttcgccaac
      481 atgatggacg gagccctcta tgctttcggt ttgagcttcg cctcgcaaca gactgtactg
      541 ccggtctttg tgcagaaaat cggcggcggt aatgtcgcca tcggcctcat cccggttctc
      601 tggatgctca gcttcaatct gccgcagata ctcatcgcca gccatgcaca gcgccaacgc
      661 cacaagaagc gactgctgct caagactgca atgggacagc gcttgccgtg gctcctgctg
      721 gctgtgctgg ccttttttat ccttcccagg gtcactcccc tggccggtct gatgcttttt
      781 tttattattt atgcctccgc agggatcggc ggcagcatca atctgccggt ctggttcgat
      841 atcttcgcca agatgacccc catctccctg cgcggccggc tcttcgcagc gcgcatgatt
      901 ctgggtggag tgctcggcat cggtgccggt atagcggtca ggcatatcct cgattggctg
      961 ccctatccgg gcaatttcgg tctgctcttt ttgctcacct ttgcggcgat gctggtctcc
     1021 tatttctttc tgaccatttt gaaggaaccg cgatctgcaa ggagcagaac tgccgcgcct
     1081 ctgctgcgcg aatacctcgg cgccctgccc agtatcatcc gggaggagcg caactatcgc
     1141 aactttctga tcgccgacgg cgccctttat cttgcctgga tggccaacgg ctttttcacc
     1201 gtgcatgccc tgtcccgttt cgatcttccc gcctccgcgg ccgggacctt taccgcgatc
     1261 atgatggcca gcaatatcgc cggcaatctc tttttcggct ggctggcgga ccatttcggc
     1321 cagcgtatca atctcatcct ctccgcggga atgatgacac tctcctgtgc gctggcaata
     1381 tttgcacccg gcccggaact ctactgtctg gtcttcgtcg gctcggccct ggccattgca
     1441 ctggtgcaga tctcgcggct gcccctcatc gctgaactct gcagtgctga acagcggccg
     1501 accttcgtcg ctctggccaa tctgatctcc tcccccttta tcatagccgg ggtggcggga
     1561 ggatggctgg ccgatcgctg cggatatgaa ttcctcttcg cctgcagcgg tctcttcgcc
     1621 ctcttttcga tgggctggta tgccagcgtc gtcagggagc cgagaggaac cgcgcacgaa
     1681 cgcgtctttt agtcggggaa aggaaaatga cccgaacttt tctgcttttt actctattgt
     1741 catgcgccgt accgcttctg gcagcccagc ttcaggaggc cccggctcca ctgcctcccg
     1801 atccccgctt caagagcgat atcctgctca tcgtcgccca tccggatgac gagaccgcca
     1861 ttggcagctg gctggccagg gcggtttatg atctccacaa gcgggtggcc atcctctatc
     1921 tcaaccgcgg cgaaggcggc ggcaactcgt tcgggaacga acagtcttca gccatgggtg
     1981 ccattcgcga aatcgaggtg cgccgcgcgg ctgcggcctt cggcatcgac ctgctctggt
     2041 ttctggatgg ccaggatact cccggccagg atctcatgca ctccctcact aactggggtc
     2101 acggcgaaaa cctcgaacgg attgtacgcc tgatccgtct cacccgtccc gaaatgatta
     2161 tcacctggct gccccactac ggcgcgggcg agaaccatgg cgaccaccag gcttcaggga
     2221 tcctcgccgt cgaggccttc gacatggccg gcgatcccac ccgtttcccc gcccagatcg
     2281 tgccgccgcg cgagcgcgca gatatcaaca attttacaga gggactgcag ccctggcaac
     2341 cgaaaaagct ctatttcttc tccgacgttt cacatcccct gccctcgacc ggccccgctt
     2401 tcaatgtgca cgagatctcc ccctcacaaa aggtgcccta ttatgagctg gcggcccgcc
     2461 tccatctgcc acacctcacc cagggggacg tcgccgagac cgccctggcc gcacaagagt
     2521 ccggcgatta ctctgccttc gctgagtgga tctccggttt caaactgatc ttcggcaagg
     2581 cggttgtgcc ctgcgcgccc tccggggatc tctttgaagg gatcaaagag ggtccgcttc
     2641 ccttcgtgca ggtgcggggc taccagccgc atcccaatgc gggtatcgcc ctcgagttgg
     2701 gcggcctctt cgcattttac cgggatttct ggcgggcgca tgagatcgaa gcgatagaaa
     2761 tcctcctcaa gccggaggtg atgatcaatg cgggcggcta tctgcacatc cccctgctgc
     2821 tcatcaataa taccagcgag tccgtgcagg tggagctgag ggcccaaatc ccggagggtt
     2881 ggcggaatgt ggccggagag gccctctata ccctgccggc gggtgctgta cggccggtgc
     2941 agaccttttt ctttgcaccg gagcggacca atcccagcgg cgaaaccatc ctctggaacg
     3001 ccaaggtggg ggagagaaca gccggctcgg tctccatgca ggtttttctt gctgaatgga
     3061 ccctgccgca gtgacaggtg tatagtcgat caaaaaatga ggttatgttg aaaagattga
     3121 ggttgaattc agcgctgcgt tggtggcttt gtctgctcct gccagcagca gtcgccgcct
     3181 gcagttgtgg ctgtggcgcc cgggagagag gcgaagagga ttatgcacgg ctggtcaacc
     3241 ctttcgtcgg cacggcgggc gggggcaata cctttcccgg cgcggtgcgc ccctggggca
     3301 tggtttcaac cagtcctcac accgcaccat ccgccccctc gggctattat catggccagc
     3361 ctttcaacta tggcttcggc atggtccatc tcagcggcac cggctgtgcc gatctcggca
     3421 gcgtcatcgt cgcggtctcg cgcggtgaaa tcaaaactcg tcctgaagag tacagatccg
     3481 ccttttctga ggagcaagcc gagcccgcgt attacagctg tcttttgcag gaaccggcgg
     3541 tccgcgtccg gacgagcgcg accgagcgct gcggcatctt tgagttcgtt cccctgcagg
     3601 atggcgagat caatatccag attgatgcgg gccgcaatct cagccgcctc ggcggtggag
     3661 cgatccggct cacccgggcc ggaggaattg agggttttaa tattggcggg ggcttttgcg
     3721 gagagaacaa ccgtcatcag attttttttt caggtgaggt gaacctgcag cccgccgacg
     3781 cgggcgtttg gcggggagag gaactcctca cagcgaacga ggcaaataca gaggaggagg
     3841 ccgtcggcgg ctggctgcgc attgcccttc gggcggggca gcccctcatg ctcaggctgg
     3901 gcatctccta tgtcagcatc gcaaacgcac gtgaaaacct gcgcgtggag atcccgcact
     3961 gggattttga acgggtgcgc agggatgggc gcgaggcctg gcaagagcag ctgtccgcag
     4021 tgcacatccg cgaggggagt gagaaggagc ggatcaaatt ctataccgcc ctttatcaca
     4081 gcctgatcca tcccggcatc atcagcgatg tcaacgggga gtatcctctc atgggccgca
     4141 gcggcaccgg ctgcaaccgg gagcgtgccc gctacagcgt tttttccctc tgggatacct
     4201 atcgtaccct ccatcccctg ctcaccctgc tctacccgag tcgtcaatcg gagatcatta
     4261 agaccatgct cgatatgtac caggaatcag gctttctgcc aaagtgggag ctcgccggca
     4321 atgagaccta tatgatggtg ggcgacgggg cggtgccggt catcgccgat tcctacatca
     4381 aggggatcga ggattt
//
LOCUS       DAOPWU010000295         4396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_20045_length_4396_cov_5.163326, whole genome shotgun sequence.
ACCESSION   DAOPWU010000295 DAOPWU010000000
VERSION     DAOPWU010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20045_length_4396_cov_5.163326"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKM23_12460"
     CDS             complement(<1..687)
                     /locus_tag="PKM23_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNY92328.1"
                     /translation="MLDLFKKFQHGLARTKSGLVEGVQRIINQARGIDEEVLEELEEL
                     LILSDLGVATTETVIENLRQRSRESGRIEPGEVLELLKQELIAQLGPGGQAAAGPEEP
                     VHQPTVISVVGVNGAGKTTTIGKLAHLHASRGRKVLLAAADTFRAAAAEQLEIWKDRA
                     GVEIIPSQSGADPASVAFDSLKAAMARHVDYLFIDTAGRLHTKENLMAELEKIHKVLA
                     RQCPGAPHEVL"
     gene            complement(733..1311)
                     /locus_tag="PKM23_12465"
     CDS             complement(733..1311)
                     /locus_tag="PKM23_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNY92329.1"
                     /translation="MKLGLMEIIKQDKYILGLPNLLSFSRLLLLPLICYAITLEPAAG
                     GGLALVLLALSFVTDFFDGYVARRRNQFSELGRILDPIMDKLNVAAIMLFLTAYRDLP
                     CWYVAIVIGRDLAILVLSLWVIKTKRHVPQSNLIGKVALVVFLIVIVTYILDLKPYNY
                     IAMAISVALVPITLIRYILVYARNGESSGSTS"
     gene            complement(1320..2492)
                     /locus_tag="PKM23_12470"
     CDS             complement(1320..2492)
                     /locus_tag="PKM23_12470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNY92330.1"
                     /translation="MKPAYDVIVIGGGPAGSMAARYAAGRGASVALFEKDREVGVPVR
                     CAEGVSHAGLAAVVENIPSRWIAQKITGAVLHAPSGAEVPLHTDQIGYILHRKIFDYD
                     LAQMAAAAGVAIFTRAHVTGLIMEQGRTAGVVVDHLGERRRIEARLVIAADGVESRAA
                     RWAGLATRTALADMESCAQATIGGYQGDPEMLHFFFSRSMAPGGYLWIFPKGNGMANV
                     GLGISASAGGERSPHDYLEAFLARFFPGASILGRVCGGVPCSNTLKELAADGLMVVGD
                     AAHQANPLTGGGIVTGMIAGRIAGETAAEAVATGRCDRETLRRYPKAWLAGEGGKQAQ
                     FYRLRKFVFSLGDEDYDALARSVLRIPVEKRTILSIMKSALLHKPSLLLDAVKLFT"
     gene            complement(2476..2658)
                     /locus_tag="PKM23_12475"
     CDS             complement(2476..2658)
                     /locus_tag="PKM23_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY92331.1"
                     /translation="MKHNQDRCDFCGVCVGVCPHDAIELREAELIVDDERCTRCGQCV
                     QACPIHALEVNDEAGV"
     gene            complement(2667..3653)
                     /locus_tag="PKM23_12480"
     CDS             complement(2667..3653)
                     /locus_tag="PKM23_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNY92332.1"
                     /translation="MTPTPPARSRLMHGLLKRLDYLFVLRPTLFYPVWTVSLAGLWSA
                     ERAAAGQPWYALHWPRQGFALEGAIAIGLLTLVMGACFLLNQITDVESDRLNNKLYLV
                     ASGAVSLRAAWSETALLLAAGLGGLLWLSRPLALVALLAFLVTGWAYSCAPLYGKDRP
                     WAGLFINLGGSGLIYIFGWLMAAPASGRMFLSGLPYIAGIGAVYLLTTIPDRPGDAAA
                     HKITLAVHWGEERIYGAALAAGTLGVLAALPVREGVALTATLLTWLFFYTAWRRRSVD
                     SALRANKFATLILSFLICWRLPVYLLLLVLVFYFSKWYYRMRFQVHYPSLLT"
     gene            complement(3669..>4396)
                     /locus_tag="PKM23_12485"
     CDS             complement(3669..>4396)
                     /locus_tag="PKM23_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNY92333.1"
                     /translation="IHDGVVESHRFAPLTPRHACIFEFVYFSRPDSRIFNENVDRVRR
                     RLGKNLALESPAEADIVISVPDSSNTAAVGYSRRTGIKFELGLIRNHYVGRTFIHPQQ
                     DMRDFNVRIKFNPVKGVLQDRRVVVIEDSIVRGTTLRQLVSLIRQAGAREVHVRVSSP
                     PIISPCYYGMDFPSTSELIAAHKSVEEIRAWLQCDSLAYLSLEGLLKSVAHDDEGYCA
                     ACFTGEYPIPVEDNYSERVHEPL"
BASE COUNT          862 a         1368 c         1430 g          736 t
ORIGIN      
        1 caggacttca tggggagctc cgggacactg gcgggcgagg accttgtgga ttttttcgag
       61 ctcggccatg aggttctcct tggtgtgcag gcgtcccgcc gtatcgatga agagatagtc
      121 gacgtgcctg gccatggcgg ccttgaggga atcaaaagcc accgaggccg gatccgcgcc
      181 gctctgggag gggatgattt cgaccccggc ccggtctttc cagatctcca gttgttcggc
      241 ggcggcggcg cgaaaggtat cagcagcggc gagcagcacc ttgcggccgc ggctggcatg
      301 gagatgggcc agcttgccga tagtcgtggt cttgccggcg ccgttgaccc cgaccacgct
      361 tatcacggtc ggctggtgca ccggttcttc cggcccggct gcggcctgcc cgccgggacc
      421 gagctgcgca atcagctcct gcttgagcag ctcgaggacc tcgccgggct cgatgcggcc
      481 gctctcacgg ctgcgctggc gcaggttttc gatcaccgtc tcggtcgtgg ccactccgag
      541 atcgctgaga atgagcagct cttccagctc ctcgagcacc tcctcatcga tcccgcgcgc
      601 ctgattgata atgcgctgga cgccctcgac cagacccgat ttggtccgcg ccaggccatg
      661 ttgaaacttc ttgaaaaggt ctaacactct cgcctcaccc ttgcaaaggt cagggctgcg
      721 cctgtgcagc cctcatgatg tgcttccgct gctttcacca ttccgggcat aaaccaggat
      781 gtagcggatg agcgtgatgg gcaccaaagc caccgagatg gccatggcga tgtaattata
      841 tggtttcagg tccagaatat aggtgacgat gacaatgaga aaaaccacca gggccacctt
      901 gccgatcaga ttcgactgcg gaacatggcg cttggttttg atcacccaga ggctgaggac
      961 caggatggcc aggtccctgc cgatgacgat ggcgacatac cagcagggca gatcgcgata
     1021 ggcagtcaga aagagcatga tcgcggcgac gttgagcttg tccatgatcg gatcaagtat
     1081 gcgtcccagt tcagagaact gattgcgccg ccgcgccaca tagccgtcga aaaaatcggt
     1141 gacgaacgag agcgcgagga gaaccagggc cagcccgccg ccggcggcgg gctcgagggt
     1201 aatcgcgtag cagatcaggg gcagaagcag cagccgcgag aaagagagca aattgggaag
     1261 gcccagaata tatttgtctt gtttgatgat ctccatcaga cccagcttca tggtgtcctc
     1321 taggtaaata gtttgaccgc gtcgaggagg agcgaaggct tgtgcaacaa agccgatttc
     1381 atgatcgaca gaatggtgcg tttttcgacg ggaatccgga gcacgctcct ggccagggcg
     1441 tcataatcct catccccgag gctgaagaca aatttgcgca gccggtagaa ctgcgcctgt
     1501 tttcccccct caccggccag ccaggccttc gggtagcggc gcagcgtctc ccggtcgcag
     1561 cggccggtgg cgacggcctc cgcggcggtc tcgccggcga tgcgtccggc gatcatgccg
     1621 gtgacgatgc cgcccccggt gaggggattg gcctgatggg cggcatcgcc gacgaccatg
     1681 aggccgtcgg cagcgagttc cttgagggta ttcgaacagg gtaccccgcc gcagacgcgc
     1741 cccaggatcg aggcgccggg aaaaaatctc gcaagaaagg cctcgagata atcatgcgga
     1801 gaacgctctc ccccggcgct ggcggagatg ccaagtccga cattggccat gccgttgccc
     1861 ttggggaaga tccagagata tccgcccggc gccatcgaac gggaaaaaaa gaagtggagc
     1921 atctccgggt cgccctgata gccgccgatg gtggcctggg cgcagctctc catatcggcc
     1981 agggcggtgc gggtggcaag tccggcccag cgcgcggcgc gtgactcaac cccgtcggcg
     2041 gcgatgacca gcctggcctc gatccgccgc cgttcgccga gatggtccac caccacaccc
     2101 gccgtgcggc cctgctccat gatcaatcca gtgacatggg cccgggtgaa gatggccacc
     2161 ccggcggccg cggccatctg cgccaggtca taatcgaaga tcttgcggtg caggatgtag
     2221 ccgatctggt ctgtatgcag cggcacctcg gctcccgagg gggcgtggag caccgcgccg
     2281 gtgatctttt gggcgatcca gcgggacggg atattctcca ccactgcggc caggccggcg
     2341 tgggagaccc cttcggcgca gcgcaccggc acgccgacct cgcggtcctt ctcgaagagg
     2401 gcgacggagg ccccgcggcc ggccgcatag cgcgccgcca tcgatccggc cggcccgccg
     2461 ccgatgacga tgacgtcata cgccggcttc atcgttcacc tccagggcat gaatcgggca
     2521 ggcctgcaca cactggccgc agcgggtgca gcgttcatca tcgacgatga gctccgcctc
     2581 gcgcagttca atggcatcgt gcgggcagac cccgacgcaa accccgcaga aatcgcagcg
     2641 gtcctgattg tgtttcattt tgttggtcat gtcaacagac tgggataatg gacctgaaac
     2701 cgcatccggt aataccattt gctgaaataa aagaccagca ccagcaggag cagatagacc
     2761 ggcaggcgcc agcagatcag gaacgaaagg atcagtgtgg caaacttgtt ggcgcgcagg
     2821 gccgagtcca cgctccggcg ccgccaggcg gtgtaaaaaa aaagccaggt cagcagggtc
     2881 gccgtcaggg ccaccccctc ccgaaccggc agggcagcca gcacccccag ggtcccggct
     2941 gccagggctg caccgtaaat gcgctcttcg ccccaatgga ccgccagggt gatcttgtgt
     3001 gccgccgcgt ctccgggccg gtcggggatc gtggtgagca gatagaccgc gccgatgccg
     3061 gcgatatagg gcaggccgga gaggaacatc cgccctgaag cgggggcggc catcagccag
     3121 ccgaaaatat agatcaggcc cgacccgccg agattgataa aaaggccggc ccaggggcgg
     3181 tccttgccgt agagcggcgc gcagctgtag gcccagcccg tgaccagaaa ggcgagcagg
     3241 gcgaccaggg ccagggggcg gctcagccac agcagcccgc ccaggccggc ggcgagcagg
     3301 agcgcggtct cgctccaggc ggcgcgcagg ctcaccgccc cgctggcgac cagatagagc
     3361 ttgttgttga ggcggtcgct ctcgacatcc gtgatctggt tgaggagaaa gcaggccccc
     3421 atcaccaggg tgagcagacc gatcgctatc gccccctcca gggcgaagcc ctggcgcggc
     3481 caatggagcg cataccaggg ctggccggca gccgctcgtt ccgcgctcca cagtccggcc
     3541 agagaaaccg tccagaccgg atagaagagg gtcggccgca gcacaaagag ataatcaagg
     3601 cgcttgagga gtccatgcat gaggcggctg cgcgccggcg gggttggcgt catgccgtcg
     3661 ttttcctctc agagcggctc atgcaccctc tcgctgtaat tatcctcgac agggatggga
     3721 tattcgccgg tgaagcaggc cgcacagtag ccctcgtcgt cgtgcgccac cgatttcagc
     3781 agcccctcca gcgaaagata ggcgaggctg tcgcactgca gccaggcgcg gatctcctca
     3841 acgctcttgt gggcggcgat gagctcgctg gtgctgggaa aatccatacc gtaatagcag
     3901 ggagagatga tcggcggcga gctgacgcgc acgtgcacct cgcgggcgcc ggcctggcga
     3961 atgagcgaaa ccagctggcg cagggttgtg ccgcgcacga tcgagtcctc gatcaccacc
     4021 acccgccggt cctgcagcac ccccttgaca ggattgaatt tgatgcgcac attaaagtcg
     4081 cgcatatcct gctggggatg gatgaaggtc cgtccgacat agtggttgcg gatgagcccc
     4141 agctcgaatt tgatcccggt gcggcgcgaa tagcccaccg ccgcggtgtt gctcgaatcg
     4201 ggcacagaga tgacgatgtc cgcctccgcc gggctctcca gggccagatt cttgcccagc
     4261 ctgcggcgta cccggtcgac gttctcgttg aagatgcggc tgtcggggcg ggagaaatag
     4321 acgaactcaa agatacaggc atggcgcggc gtcagcggag cgaagcggtg cgattcgacc
     4381 accccgtcat ggatct
//
LOCUS       DAOPWU010000296         4360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_20272_length_4360_cov_5.693844, whole genome shotgun sequence.
ACCESSION   DAOPWU010000296 DAOPWU010000000
VERSION     DAOPWU010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20272_length_4360_cov_5.693844"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /locus_tag="PKM23_12490"
     CDS             complement(<1..1774)
                     /locus_tag="PKM23_12490"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNY92334.1"
                     /translation="MSLAEYKSYWHRLRAMSPAEAAARLRGNLAGRWSRRLDQRPAPP
                     LAALLAHPPLAPEALLASARARRLLSFELELPPRPGVWFAARWPEAHAALLDSAARAA
                     GRRFTLFAAELSFTGPIDWHYDPVAQRSMPLSHWTAIPYWRPGYCPGVKEIWELNRCQ
                     HFVTLGQVWRLAGEEEYARALLEQWQGWLDGNPAGRGINWTSMLELGLRLISWSWALA
                     LVKNSPLLSAEFYARLLVSVHQQAAHISGHLSRGSSANNHLLGEALGLVYAGTCFPEL
                     KGAAAWRKQGFHLFWSELVRQVHPDGVIKEQSTGYMRYLYDYGTLAITAAAAAGEGVP
                     PAVLQRMAGMAEFTAALIDEAGRLPQIGDGDDGQALLLDPAAGHAAPSPWRDLLGSAA
                     LQFGRADFKALSAGFSPTLFWLHGAAAEGAFARIPTPAGAPPSLRLFPEGGYAVLRSE
                     AESGRQIGLLDAGPLGLDAMAAHGHADALSFWLTAGGEPLLIDSGTYQYRGEESWRRY
                     FRSTAAHNTVRVAGRDQSQILGPFQWGRRAQARLLASSAAEASLAIAGEHDGYRSLGV
                     IHRRRVSLEEGLWVVEDELLGRGRA"
     gene            complement(1777..2970)
                     /gene="dinB"
                     /locus_tag="PKM23_12495"
     CDS             complement(1777..2970)
                     /gene="dinB"
                     /locus_tag="PKM23_12495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNY92335.1"
                     /translation="MSRTILHIDMDAFFAAVEQNDHPEWRGKPVVVGSDPQGGRGRGV
                     VSTCSYEARRFGIHSAMPISRAWKLNPEAIYVRPRGRRYAGVSRQVMNLLSSFSPDIE
                     QLSIDEAFLDITSTQKLFGGTEAMAVRIKAEIRAQTNLTASIGVAPNKFVAKIASDLR
                     KPDGLVIVPEGGVEAFLAPLEISRLWGCGPKTVPVMQAMGIFTIGDLAARSQKELVDR
                     FGQTGLHFWRLARGLDERPVSDAHTARSMSHETTFMTDCGDEEEMRKTLLGLCDDLAY
                     DMRRHQVRGRTITLKIRLADFSTFTRSHSLERATDSSAELYQQALQLFSSFRREGQRV
                     RLLGVALSQLQQGEGQMELFREGDGRSEKVDSIMDDVRKKFGAGAISRASLLHKRSDS
                     QWIRE"
     gene            complement(2990..3409)
                     /locus_tag="PKM23_12500"
     CDS             complement(2990..3409)
                     /locus_tag="PKM23_12500"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNY92336.1"
                     /translation="MRIYFAASISGGRRYLPACTRMVEFLKDLGHTVLTEHIVAPDVL
                     SGEMNFTAQQIYERDTAWLESCDRVVAEVSNPSLGVGYEICYALHFGKPVIALHEEGL
                     FISRMITGISQPGITVTAYRDEAGWRESLIRFLEDAR"
     gene            complement(3414..4256)
                     /locus_tag="PKM23_12505"
     CDS             complement(3414..4256)
                     /locus_tag="PKM23_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY92337.1"
                     /translation="MPEEAKAALKEFYQQVGEKYPEEEEVYRTLRGMLRREFILGRLA
                     GLKGTFLDVGCNRGSYLESWQGGPRFGLDISLGALRQSPAPLAGRLVQGDAERLDCFR
                     PGSFDHLLCSEVLEHCLHPGMIFTGIALVLKPGGQALLTTPNYHGRRPEHLPLGILEE
                     YGVECSCGGSYFHTAYRPGELEAMARAAGLEVAESGTLEKEVKYAAKLPAALLLFVRW
                     INRFLRWPRLGRANEAFFHTFTLACHRFCAATRLERLLLPLVKEGVRSYIVMRKPCTQ
                     MEPR"
BASE COUNT          779 a         1375 c         1513 g          693 t
ORIGIN      
        1 ccgcccttcc cctgcccagc agctcatcct cgaccaccca gagtccctcc tccaagctca
       61 cccggcggcg gtggatgacg ccgagactcc ggtagccgtc gtgttcaccg gcgatggcga
      121 ggctggcctc cgccgcgctg gacgccagca ggcgggcctg cgcccgccgg ccccactgaa
      181 aggggccgag gatctgcgat tgatcgcggc ccgccacccg cacggtgttg tgcgcggcgg
      241 tggagcgaaa gtagcggcgc cagctctcct cgccgcgata ttgataggtg ccgctgtcga
      301 tgagcagggg ctcgccgccg gcggtgagcc aaaagctcag ggcatcggcg tggccgtgcg
      361 ccgccatggc atccaggccg agcggaccgg catcgagcag gccaatctgg cggccgctct
      421 ccgcttcact gcgcagcacc gcatagcctc cctcaggaaa gaggcgcagg gacggcgggg
      481 cgccggcggg ggtgggaatg cgggcgaaag cgccttcagc cgccgcgcca tgcagccaga
      541 agagggtggg cgaaaagccg gcggaaaggg ccttgaaatc agcacgcccg aactgcaggg
      601 cggccgaacc gagcagatcg cgccagggtg agggagcggc atgcccggca gcgggatcga
      661 gaagcagggc ctggccgtca tccccgtccc cgatctgcgg caaacgcccc gcctcatcaa
      721 tgagggcggc ggtgaattca gccatccctg ccatccgctg cagcacggcc gggggaacgc
      781 cttctccggc tgctgcagca gctgttatcg caagcgttcc ataatcatat agataacgca
      841 tataaccggt gctctgttcc ttgatcaccc cgtcgggatg cacctggcgc accagctcgc
      901 tccagaagag gtggaagccc tgcttccgcc aggccgcagc ccccttcaac tccggaaagc
      961 aggtgccggc gtagacaagc ccgagcgcct cgccgaggag gtggttgttg gcagaagatc
     1021 cgcgcgagag gtggccggag atatgagcgg cctgctggtg gaccgaaacg agcaggcggg
     1081 cgtagaattc ggcgctgagg aggggcgaat ttttaaccag ggccagcgcc cagctccacg
     1141 agatcagccg caggccgagt tcaagcatgc tggtccagtt gatgcccctt ccggccggat
     1201 tgccgtcgag ccagccctgc cactgctcga ggagggcgcg ggcgtactcc tcctcccctg
     1261 ccagccgcca gacctggccg agggtgacga agtgctggca gcggttgagt tcccagatct
     1321 ccttgacccc ggggcagtaa ccggggcgcc agtaggggat ggccgtccag tggctgaggg
     1381 gcatggagcg ttgggcaacg ggatcgtaat gccagtcgat cggcccggtg aaggagagtt
     1441 ccgcagcgaa gagggtgaag cggcggccgg ccgctctggc ggcgctgtcg agcagggcgg
     1501 catgagcctc aggccagcgc gcagcgaacc acaccccggg gcggggcggc agctccagct
     1561 cgaaggagag gaggcgccgc gcgcgggcgg aggcgagcag cgcctcagga gcgaggggcg
     1621 ggtgggcgag caaggcggcc aggggcggcg ccgggcgttg atccagacgg cgcgaccagc
     1681 gcccagcaag gttgccgcgc agacgggccg ccgcctcggc ggggctcatc gcccgcaggc
     1741 gatgccagta gctcttgtac tcggccaggg acatccctac tccctgatcc actgcgaatc
     1801 actgcgcttg tggagcaggc tggcgcggct gatcgccccg gcgccgaact ttttgcgcac
     1861 atcgtccata atgctgtcca ccttctcact gcgcccgtcc ccctcccgga aaagctccat
     1921 ctgcccctcg ccctgctgaa gctgggagag ggcgaccccg agcagccgca cccgctggcc
     1981 ttcccgcctg aaggaggaaa aaagctgcag ggcctgctgg taaagctccg cgctgctgtc
     2041 ggtcgcccgt tcgagggagt gggagcgggt gaaggtgctg aaatcggcga ggcggatctt
     2101 gagggtgata gtgcggccgc gtacctggtg gcggcgcata tcgtaggcga gatcgtcgca
     2161 gaggccgagg agggttttgc gcatctcctc ctcgtcgccg cagtcggtca tgaaggtggt
     2221 ctcgtggctc atcgaacggg cggtgtgggc gtcggagacc gggcgctcgt cgaggccgcg
     2281 cgccagccgc caaaaatgga gcccggtctg gccgaaacgg tcgaccagct ccttctgcga
     2341 gcgggcggcg aggtcgccga tcgtgaagat gcccatcgcc tgcatcaccg gcacggtttt
     2401 gggaccgcag ccccagagcc ggctgatctc gaggggggcg agaaaggcct cgactccgcc
     2461 ctccgggacg atgaccagcc cgtccggctt gcgcaggtcg gaggcgatct tggcgacgaa
     2521 tttgttgggg gcgacgccga tcgatgcggt caggttggtc tgggcgcgga tctccgcctt
     2581 gatgcgcaca gccatggcct cggtcccgcc gaagagtttt tgtgtggagg tgatgtcgag
     2641 aaaggcctca tcgatgctga gctgttcgat atcgggactg aaactggaaa ggaggttcat
     2701 cacctggcgc gagaccccgg cgtagcggcg gccgcgcggc cggacataga tggcctcggg
     2761 attgagcttc caggcccggg agatgggcat ggcggagtgg atgccgaagc gccgtgcctc
     2821 atagctgcaa gtggagacca cgccgcggcc ccggcccccc tggggatcgg agcccaccac
     2881 caccggcttg ccgcgccact cgggatggtc gttttgctcc acggctgcga aaaaggcatc
     2941 catatcgata tgaaggattg ttctggacat ggcagctccc cctgctcggt caacgagcat
     3001 cctccaggaa acggatgagg gactcccgcc agcccgcctc gtcccggtag gcggtgacag
     3061 tgatgccagg ttggctgatg ccggtgatca tgcgcgagat gaaaagcccc tcttcatgga
     3121 gggcgatgac cggcttgccg aaatggaggg cgtagcagat ctcatagccc acccccaggg
     3181 agggattgga gacctcggcc accacccggt cgcacgactc gagccaggca gtgtcgcgtt
     3241 catagatctg ctgcgcggtg aagttcatct ccccgctcaa cacatccggg gcgacgatgt
     3301 gttcggtcag aaccgtatgg ccgaggtcct tgagaaactc caccatgcgg gtgcaggcgg
     3361 gcagatagcg tcttccgccc gagatcgacg cggcaaagta gatgcgcatg gcttcagcgg
     3421 ggctccattt gggtgcaggg tttacgcatg acaatatagc tgcgcacccc ctctttgacc
     3481 agggggagga gcagccgttc cagcctcgtt gccgcgcaga agcggtggca ggcgagggtg
     3541 aaggtatgga aaaaggcctc gttggcgcgg ccgagccgag gccagcgcaa aaaacggttg
     3601 atccagcgca cgaaaaggag aagggcggcc ggcagcttgg cggcatattt gacctccttt
     3661 tcgagtgtgc ccgattcagc cacctcgagt ccggccgcgc gggccatggc ctcgagctcc
     3721 cccggccggt aggcggtatg aaaatagctc ccgccgcagc tgcactctac gccatactcc
     3781 tcgaggatgc cgagggggag atgttccggc cggcggccgt gatagttggg cgtggtcagc
     3841 agggcctgcc cgccgggttt cagaacgagg gcgatgccgg taaaaatcat gccgggatga
     3901 aggcagtgct cgagcacctc gctgcagagc agatggtcga acgagccggg gcggaagcag
     3961 tcgaggcgtt cggcgtcgcc ctggacgagg cggccggcga ggggagccgg gctctggcgc
     4021 agggctccga ggctgatgtc caggccgaag cggggcccgc cctgccagct ctccaggtag
     4081 ctgccgcgat tgcagccgac gtcaagaaaa gtgcctttca aacctgccag acggccgagg
     4141 atgaactcgc gccggagcat gccacgcagg gtgcggtaga cctcctcctc ctcaggatat
     4201 ttctcgccga cctgctgata aaactccttc agcgcggcct tggcctcttc gggcatccaa
     4261 tcctctcgtt tttctccatg ccatcccggc gtcggggtgc gtacagcgct caaatttaca
     4321 taattttgcg gaaaaaaccc cgtataattt cgcgctcctc
//
LOCUS       DAOPWU010000297         4357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_20287_length_4357_cov_4.428405, whole genome shotgun sequence.
ACCESSION   DAOPWU010000297 DAOPWU010000000
VERSION     DAOPWU010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20287_length_4357_cov_4.428405"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKM23_12510"
     CDS             complement(<1..336)
                     /locus_tag="PKM23_12510"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNY92338.1"
                     /translation="MSQNFHALIPEIKSRIKEECVRETPQDNETLIGLPYPYVISGKG
                     KQEALYYWDTYFINLGLLRMRMIDYARHNVENLIFLLRKFGYVPASNAREMLTHSQPP
                     FLPWMVRDIY"
     gene            complement(637..1458)
                     /locus_tag="PKM23_12515"
     CDS             complement(637..1458)
                     /locus_tag="PKM23_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNY92339.1"
                     /translation="MLIGFTVEGYQDWHPAAITAAMRRLGVNFIEFNLRVVDELERVL
                     PQLRGMRTAFHLPITDEDGWDLASTERQEKIDQLIANLQSHHHQLGLTHFIAHPPESA
                     PDSPAWQAGLESLFAATARLPRPTCFENVITTSPEQIRDFLGEAKRRLGDLYAGMCFD
                     AAHFMISGHDPVEQFIAFSDEIGSVHLSDCIGREDSHLPFGSGGGLPIKKLLRTMRRS
                     GYAGTITLEIRPTAGGDLYAFIESYMLLLRYFAPGRYLAARLRLFAMRHILRRLK"
     gene            complement(1551..1970)
                     /locus_tag="PKM23_12520"
     CDS             complement(1551..1970)
                     /locus_tag="PKM23_12520"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNY92340.1"
                     /translation="MKISIRSTKYSDLEIGLLLLLFGLIGMAISHFLPGVAERLPRCL
                     FRAWTGLPCPSCGATHAGIALSRAQLWSALRFNPLFTLLYIGMAVTAANALAGLIFGR
                     NLAVTWSCTARPLLLKALFSALLLNWAYLLGQRFAAM"
     gene            2106..3032
                     /locus_tag="PKM23_12525"
     CDS             2106..3032
                     /locus_tag="PKM23_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNY92341.1"
                     /translation="MRRWYEQAGIALLAGILLAAGGVTAQQIETRTVVTGGAWLGVYL
                     GDAGEESGWDESEGAIVREVVKDGPADKAGLRENDIIIKLQDRTVRDADDVTRDIRKM
                     KPGDEVKLLVLRDKKKLTLTAKLQERKRVREESLPPAVDEEAKKEFERQRDLRPLQGL
                     WMQKNRRMGVQLQELDSDLAEYFQTGEGVGVLVTGVEKESPAAAAGLKSGDVITAVNG
                     KKVRKAGDVVKALEENESGEQEVAFLRKGSARSVIVKLPERPERNFRIQLEGSGWPFS
                     RETMEELREDLEELRQELDQLKIKLNIQVDEK"
     gene            3253..4311
                     /gene="asd"
                     /locus_tag="PKM23_12530"
     CDS             3253..4311
                     /gene="asd"
                     /locus_tag="PKM23_12530"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNY92342.1"
                     /translation="MDKIRVAVLGATGMVGQYFLHLLHDHPFFQVAAVCASDARIGQL
                     LGQVRPLHPAGIAPELAEMRFTSMDADAIAALEVQAAFSGLPAEIARTIEPALAGCGI
                     HIFSNASAFRMDPAVPILIPEINHHHLALLPRQQHKGRIVTNANCTSTGLALSLAPLR
                     ELSIRRLIVASYQAVSGAGYPGVPALDISGNLIPWIEGEEGKVRQECNKIFGCIGAGG
                     LEPAGWEVHAHCVRVPTLVGHLVSTHIELNREVTAAEVEECYSSFQTPVEVAGLPSAP
                     VRPVLLTHEAGRPQPARDIEAGEPGRTSGMAVVVGRLEVSGSVIRQVNLANNLVRGAA
                     GGSVLNAELALRAGLFLH"
BASE COUNT          893 a         1194 c         1446 g          824 t
ORIGIN      
        1 gtagatgtcg cgcaccatcc agggaagaaa gggcggctgg gagtgagtga gcatttcgcg
       61 ggcgttggaa gcgggtacgt agccgaattt gcgcagaaga aagatgagat tctcgacatt
      121 gtggcgcgcg tagtcgatca tgcgcatgcg cagcaatccc agattgatga aataggtgtc
      181 ccaataatag agggcttcct gtttgccttt gccggagatg acataagggt agggcagtcc
      241 gatcagggtt tcgttgtcct gaggggtctc gcgcacgcac tcttccttga tgcgcgattt
      301 gatttctggg atcaatgcat gaaaattctg actcattccc aacctcactt ttttgagtgc
      361 ttttgctcta aagatgctgc cggcggagcg ccgcagcagg cttgcggtgc taatgcacca
      421 ggatgccgtt cacgtgatgg cacaggatcc tgaaggaata ggatgcaggt gaagcgaagg
      481 gatttggggg tgtgtttggt ctcagtcatt ttgctgtgtg ggatcggctc aaagagagtg
      541 gataagcctc taattgaaag taacgccgtg gatggataaa agcaagcaat atttccggga
      601 taagcgcaat atcatcaaga tataatatcg gccggtctat ttcagccggc gcaggatgtg
      661 ccgcatggca aagaggcgca gccgggcagc gaggtagcgg ccgggcgcga agtagcgcag
      721 caggagcata tagctttcga taaaagcgta gaggtcgccc cccgcagtgg ggcggatctc
      781 cagggtgatg gtgccggcat acccgctgcg gcgcatggtc cgcagcagct ttttgatcgg
      841 caggccgccc ccactgccga aggggaggtg gctgtcctcc cggccgatgc agtcgctgag
      901 atgaacggag ccgatctcgt cgctgaaggc gatgaactgc tccaccggat catggccgct
      961 gatcatgaaa tgggcggcgt cgaaacacat gccggcataa aggtcgccca ggcgtctttt
     1021 agcttcgcca aggaagtcgc ggatctgttc cggagaggtc gtgatcacat tttcgaaaca
     1081 ggtgggccga ggcagccgcg cggtcgcggc gaagagactt tccagaccgg cctgccaggc
     1141 cggggaatcg ggagccgatt cgggtggatg ggcaatgaag tgggtcaggc cgagctggtg
     1201 atggtggctt tgcaggttgg cgatgagctg atcgatcttc tcctgcctct ccgtgctggc
     1261 gagatcccag ccgtcctcgt ccgtgatcgg cagatgaaag gcggtgcgca tcccccgcag
     1321 ctgggggaga acgcgttcca gctcgtcgac gacacgcagg ttgaattcaa taaagttgac
     1381 ccccaagcgg cgcatggccg cagtgatcgc cgccgggtgc cagtcctgat agccttctac
     1441 agtgaagccg atgagcacgg ttctctctcg aaaagtgttc ctgcgccagt ctttcttaca
     1501 aaaccccatt tggcggttgt ttgttcccgt gttgacgcag cgccggaccc tcacatcgca
     1561 gcgaagcgtt gtccgagcag ataggcccag ttgagcaaca gtgcggagaa gagtgccttg
     1621 aggagaaggg ggcgggcggt gcagctccag gtcacagcca gattgcggcc gaaaataagc
     1681 ccggcgaggg cattggcggc ggtgacggcc atgccaatat acaagagggt gaagagggga
     1741 ttaaagcgca gggcactcca gagctgggca cggctgagtg cgatgccggc atgggtcgct
     1801 ccgcaggaag ggcagggcaa gcccgtccag gcgcggaaga gacagcgcgg cagcctttct
     1861 gcaacccccg gcaggaagtg gctgatcgcc atccctatca gaccgaacag gaggagaaga
     1921 agcccgatct cgagatcgga atattttgtg gaacgaattg aaatcttcat gcgtatcaaa
     1981 agcaaaagga ttgacaggcg gagttgtcgg tgccggcccg agtgcaaatc cgccggcagt
     2041 gaaaaaagat acactgccgc gccgtcaaaa acaagataat tattgggtta ttcaatggag
     2101 gacatatgag acgatggtat gaacaggcgg gaattgccct gctggcagga attctgctgg
     2161 ccgcaggtgg tgttaccgcc cagcagatcg aaaccaggac agtcgtcacg ggaggcgcct
     2221 ggctgggtgt ctacctcggt gacgccgggg aggagagcgg ctgggatgaa agtgagggcg
     2281 cgatcgtgcg ggaggtggta aaggatggac cagccgacaa ggcagggctg cgcgagaacg
     2341 acatcatcat caagctgcag gaccgcaccg tgcgcgacgc tgacgatgtg acccgggaca
     2401 tccgcaagat gaaaccgggc gatgaggtga agctgctcgt cctgcgcgac aagaaaaagc
     2461 tcaccctcac cgccaaactg caggagcgca agcgcgtgcg cgaagagagc ctgcctccag
     2521 cagttgatga agaggccaag aaggagttcg aacggcagcg ggatcttcgc cctctgcagg
     2581 ggctgtggat gcagaagaac cgtcgcatgg gggtgcagct gcaggaactg gacagcgacc
     2641 tcgccgagta ttttcagacc ggagagggtg tgggtgtgct ggtgaccggg gtggagaagg
     2701 agagccccgc ggccgcggcc ggactcaaat cgggtgatgt catcactgcc gtgaacggta
     2761 agaaggtgcg caaggccggg gacgtggtca aagccctgga ggagaacgag agcggtgaac
     2821 aggaggtcgc tttcctgcgc aaggggagtg cccggtccgt gatcgtcaag ctgcccgagc
     2881 gcccggagcg gaatttccgc atccagctgg agggcagcgg ctggccattc agccgggaga
     2941 cgatggagga actgcgcgag gatcttgagg agctgcggca ggagctcgac caactcaaga
     3001 tcaagctcaa catccaggtg gatgaaaaat agcggaagcc ccggggctgc cgctgcatca
     3061 aggccggagc tctctccggc ctttgttgtt tccaggcggt ccggcgctga gctgttgcat
     3121 ccgcccttca gcaccggttg cgatgacccg gcttcagcgg cggctgcttc atgcgggtgg
     3181 gcggagtatt ccgcccttgt ttttctcgct ccctttattt acatttaatg aaaaatcctt
     3241 tcggaaagag ccatggacaa gatccgcgta gctgttctcg gcgccaccgg catggtgggc
     3301 cagtattttc ttcatctcct ccatgaccac ccctttttcc aggtggcggc ggtctgcgcc
     3361 agtgatgccc gcatcggcca gctccttggg caggtcaggc cccttcaccc ggcggggatc
     3421 gcgccggagt tggcggaaat gcgttttacc tccatggacg ccgacgccat cgccgccctc
     3481 gaggtccagg ccgccttttc aggactgccg gccgagatcg cccgcacgat cgagccggcc
     3541 ctggccgggt gcggcatcca tattttttcc aatgccagcg cctttcgcat ggatccggct
     3601 gtgcccattc tgattccgga gatcaaccac caccatctcg ccctcctccc ccggcagcag
     3661 cacaagggga ggatcgtgac caacgccaac tgcacgagca cgggactggc gctctccctg
     3721 gctcccctgc gggaactctc catccgccgg ctcatcgtcg ccagctatca ggcggtttcc
     3781 ggggccggct atcccggcgt gccggcgctg gacatctccg gcaacctcat tccctggatt
     3841 gaaggcgagg agggcaaggt gcgccaggag tgcaacaaaa ttttcggctg tatcggggcc
     3901 gggggcctcg aaccggccgg ctgggaggtt catgcccatt gtgtgcgtgt gcccaccctg
     3961 gtgggccatc tggtctctac ccatattgaa ctcaaccgcg aggtgacggc ggcggaggtg
     4021 gaggagtgtt acagttcctt ccaaacgcct gtggaggtgg cgggcctgcc cagcgcgcct
     4081 gtcaggccgg tgctgctcac gcacgaagcg ggccgcccac agccggcgcg ggatatcgag
     4141 gccggggagc ccgggcgcac gagcggcatg gctgtggtcg tgggtcgcct cgaggtcagc
     4201 ggttccgtca tccgtcaggt caacctggcc aacaatctgg tccgcggcgc tgccggcggc
     4261 tcggtgctca acgccgaact ggccctgcgc gcaggccttt ttttgcacta atgcttatcg
     4321 atcgggattg atcatgatcg ttatgaaaat cggcggc
//
LOCUS       DAOPWU010000298         4345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_20351_length_4345_cov_3.357809, whole genome shotgun sequence.
ACCESSION   DAOPWU010000298 DAOPWU010000000
VERSION     DAOPWU010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20351_length_4345_cov_3.357809"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1254)
                     /locus_tag="PKM23_12535"
     CDS             complement(64..1254)
                     /locus_tag="PKM23_12535"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNY92343.1"
                     /translation="MTEKLYQQDSYLQEMEARVVKRGEREGRPALVLDRTVFYPTSGG
                     QMHDTGRLNDVAVVDVQIAEEEIWHLLEQPLASEQLHGRIDWPRRFDFMQQHTGFHLL
                     AGAFFQGFGIRTLAAHLGEEESTIEIDAPEVTTAMIEEVETLANRIIWEDRPVGSRLV
                     SRAEAEKLQLRKAPQVEGEIRLIDIAGFDLDPCGGTHVSSTGQVGVVKIISRERIRQG
                     TRCTFVAGGRAWRLIQNFSQILDELTRLLTTDRGSLFTVVSKLQEENRSLRKTLQQKN
                     QLLAEQALEALCTAAGEAPIISHIFTESDLELVRSLAANAVRKRAGIYLFAGASARAA
                     LVFAASAADLDLRPAFTAAMQIVEGRGGGEAAFLQGSGSRVDKIGEALRTARELLEKP
                     DKKD"
     gene            complement(1251..2486)
                     /locus_tag="PKM23_12540"
     CDS             complement(1251..2486)
                     /locus_tag="PKM23_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNY92344.1"
                     /translation="MMSIREKLAALQRTTPTAPSPPQERAPLRGLEEIGLECTETLHG
                     PLWIRHKRFTAGQILPYRLEDFLQLEPRQFLLIGKNHALQGVHPGKLLFIDTETTGLA
                     GGAGTYAFLVGIAICSEEAVEVRQYFLVDLDAEKALYSSLASEFGGREAIASYNGKSY
                     DLPLIRSRCVMNRINFDGLDLPHLDLLHAVRRLYKGFASYTLGEVEGRLLHIRREEDI
                     PGALIPGLYFEAVRSGDLTTLKPVFQHNVMDLLSLIGITAAAAGRFDAHEGLPARDLL
                     AVIRTLTDLQFYGDAERIGCTALCTPAEEGWTSLARHRAGLHKRRGGYAEAAEIWKEW
                     IEQAPDFSFEPYEELAKYHEHRAGDIAAALDILARAEGRLEIARALHDHYVYREWEAS
                     LRHRKERLLRKQQLQRREA"
     gene            complement(2494..>4345)
                     /locus_tag="PKM23_12545"
     CDS             complement(2494..>4345)
                     /locus_tag="PKM23_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNY92345.1"
                     /translation="RTSGHIVVTNPDMLHTGILPHHSIWVKLFENLRYIVIDEIHNYR
                     GVFGSHFANVLRRLKRICAFYGSQPRFICCSATIANPGDFARYLTGEPLCLVDDNGAP
                     RGRKHFIFYNPPLVNRELGIRRSCVLEAQKVAGKFLGHDVQTIVFARSRMRVEILLTY
                     LKEFMRRIKKPEHLVRGYRGGYLPLERREIEHGLRSGEILGVVSTNALELGIDIGQLQ
                     VCIMAGYPGTIASAWQQAGRAGRRSEAALAVLVASSSPLDQFIIHHPDYLFQQSPEAG
                     IINPDNLIVLLSHIKCAAFELPFAFDERFGSRWDGGEGVDSTQEILAYLEENRVVHKA
                     AERWHWMAEVYPAEAISLRSAAEENVVIIDKTTPQERVIGEIDLFAAPLMVHDEAIYM
                     HGSRQYHVDKLDWERRKAYVREVAVDHYTDAQLKSDLRVMDILEEKSIGSHLAGYGEV
                     SVTSVATMYKKIKFGTHENIGWGKINLPESTMHTMAFWYAFPETIHEELGIPQQEFSD
                     GLKAAANVLGHIAPLFIMSDPGDISVLPMMRATLWHKPSLFIWERYPGGVGHSAKLFQ
                     IYEEVARAGLELVQGCGCQNGCPSCSGPALEVGESGKLHAVKVLQAMIAW"
BASE COUNT          789 a         1278 c         1383 g          895 t
ORIGIN      
        1 agctgagcta tagccccatc ttttctattg tggcatttaa tttataaaaa aaatccctca
       61 ttgtcaatct tttttatcag gtttttccaa cagctcccgg gcggtccgca aggcctcgcc
      121 gattttgtcc acccggctgc cgctgccctg caggaaggcg gcctctcccc caccccgtcc
      181 ctcgacgatt tgcatggcgg cagtaaatgc cggcctgaga tcgaggtccg ctgcgctggc
      241 cgcaaaaacc agagcggcgc gcgcagaggc tcccgcgaag aggtaaatcc ccgcacgttt
      301 gcgcacagcg ttggctgcga gtgaacgcac cagctccaga tcgctttctg tgaagatgtg
      361 gctgatgatg ggggcctctc ccgccgcagt gcacagagct tccagggcct gctcggccag
      421 gagctggttc ttttgctgca gggtcttgcg caggctgcgg ttttcctcct gcagtttcga
      481 cacgacggtg aacaggctgc cgcgatcggt ggtcaacagg cgggtcagct catccaggat
      541 ctgactaaag ttctggatga ggcgccaggc gcgccccccg gccacaaagg tacagcgcgt
      601 gccctggcgg atgcgttcgc ggctgatgat tttaaccacc cccacctgac cggtgctgct
      661 gacatgggtg ccgccgcagg ggtcgaggtc aaaaccggcg atgtcgatga ggcggatttc
      721 cccctccacc tggggggcct tgcgcagctg cagtttttcc gcctccgccc ggctgacgag
      781 gcggctgccc accggccggt cctcccagat gatgcggttg gccagggttt cgacctcctc
      841 gatcatggcc gtggtcacct ctggggcgtc gatctcgata gtggattctt cctcgcccag
      901 gtgagcggcc agggtgcgga tgccgaatcc ctgaaaaaag gccccggcca ggagatgaaa
      961 gccggtgtgc tgctgcataa agtcgaaacg ccgcggccag tcgatacggc cgtgaagctg
     1021 ctcggacgca agtggctgct caagcagatg ccagatctcc tcctctgcga tctgcacatc
     1081 caccaccgcc acatcgttga gccggccggt atcgtgcatc tggcctccgg aggtgggata
     1141 aaagaccgtg cgatccagga ccagcgccgg ccgcccctcc cgctcccccc ttttgaccac
     1201 tctagcctcc atctcctgca gatagctgtc ttgctgatag agtttttcag tcacgcctct
     1261 ctcctttgaa gctgttgttt gcgcagcagc ctttccttgc ggtgcctcag gctggcttcc
     1321 cactctcgat agacatagtg gtcgtgcagc gcccgcgcaa tttccagcct tccttcagcg
     1381 cgggcgagga tgtcgagcgc agcggcgatg tcgccggccc ggtgttcgtg gtatttggcg
     1441 agttcttcgt agggctcaaa actgaaatcc ggagcctgtt cgatccactc tttccagatt
     1501 tccgccgctt cggcatagcc gccccggcgt ttgtgcagtc cggcccgatg ccgggccagg
     1561 gaggtccatc cctcctctgc aggggtgcag agggctgtgc agccgatgcg ttcggcgtcg
     1621 ccgtagaact gcagatccgt gagagtgcgg attacagcga gcagatccct tgccggcagc
     1681 ccctcgtggg cgtcaaagcg cccggctgcc gctgcggtga tgccgatcag gctgagcaga
     1741 tccatgacgt tgtgttgaaa aaccggcttc agggtggtca gatcgccgct gcgcacggcc
     1801 tcgaaataga gccccggaat gagggcgccg gggatatcct cctcccggcg gatatgaagg
     1861 agccggccct ccacctcgcc gagggtgtag gaggcaaatc ccttgtaaag gcgtctgacc
     1921 gcatggagaa gatcgagatg gggcaggtcc aggccgtcga aattgatccg gttcatcacg
     1981 cagcggctgc ggatgagggg gagatcgtag cttttgccgt tatagctcgc gatcgcctcc
     2041 ctcccgccga actcgctggc gagactgctg taaagggctt tttcggcatc gagatcgacg
     2101 agaaagtact gccggacctc caccgcctcc tctgagcaga tggcaatccc gaccagaaaa
     2161 gcataggtgc cggcgcctcc ggcgaggccg gtggtttcgg tatcgatgaa gagcagcttg
     2221 ccgggatgga ccccttgcag cgcatggttt ttgccgatca ggaggaactg ccttggttcg
     2281 agctggagga aatcctcaag ccggtagggg agaatctgtc cggcggtgaa gcgcttgtgt
     2341 ctaatccaga gtgggccatg gagggtttcc gtgcactcca gtccgatctc ctcgagtccc
     2401 cgcagaggag ctctctcctg cggcggcgaa ggcgcggtgg gtgtggtccg ctgcagagcg
     2461 gccagttttt ccctgatcga catcatgggt ttttcaccat gcgatcatgg cctgcagcac
     2521 cttcacggcg tggagtttgc cgctctcgcc cacctccaga gcgggtcccg agcaggaggg
     2581 gcagccgttc tggcagccgc agccttgcac cagttccagt ccggcgcggg ccacctcctc
     2641 ataaatctga aagagtttgg cgctgtggcc gacgccgccg gggtagcgct cccagatgaa
     2701 gagggatggc ttgtgccaga gggtggcgcg catcatgggc aggaccgata tatccccggg
     2761 atcgctcatg atgaagagcg gggcgatatg gccgagcaca ttggcagcgg ctttgaggcc
     2821 gtcgctgaac tcctgctggg ggatgccgag ctcttcgtgt atggtctccg ggaaggcata
     2881 ccagaaggcc atagtgtgca tggtcgattc gggcaggttg atcttgcccc agccgatatt
     2941 ctcgtgggtg ccgaatttga tctttttgta catggtcgca acgctggtga ccgagacctc
     3001 gccgtagccg gccagatggc tgccgatgct cttctcctcg aggatatcca tcaccctgag
     3061 gtcgctttta agttgggcat cggtgtagtg atcgactgca acctcacgga cataggcctt
     3121 gcgccgctcc cagtcgagtt tgtcgacgtg gtactgccgg ctgccgtgca tatagatggc
     3181 ctcgtcatgg accatgagag gggcggcgaa gagatcgatt tcaccgatga cccgctcctg
     3241 gggggtggtt ttgtcgatga tgaccacatt ctcctcggcg gcactgcgca ggctgatcgc
     3301 ctcggccggg tagacctcgg ccatccagtg ccagcgttca gcggccttgt ggacgacgcg
     3361 gttttcctcg agataggcga ggatctcctg ggtggaatcg acgccctctc caccatccca
     3421 gcggcttccg aagcgctcgt cgaaggcgaa ggggagctcg aaggcggcgc atttgatatg
     3481 gctgagcagg acgatgaggt tgtccggatt gatgatgccg gcctccgggc tctgctggaa
     3541 gagataatcg gggtggtgta tgatgaactg gtccagaggt gaactcgagg cgacgagcac
     3601 cgccagggct gcctcgctgc gccggcctgc acgccccgcc tgctgccagg cgctggcgat
     3661 ggtgccggga taaccggcca tgatgcagac ctggagttgg ccgatatcga tgccgagctc
     3721 cagggcgttg gtgctcacta ctccgaggat ctccccgctg cgcagaccat gctcgatctc
     3781 gcggcgctcc aggggcagat agccccccct gtagccgcgt accagatgct ccggtttctt
     3841 gatgcgtcgc atgaactctt tgagataagt cagcaagatt tcgacgcgca tgcggctgcg
     3901 ggcgaaaacg atggtttgca catcatggcc gaggaattta ccggcgacct tttgcgcctc
     3961 caggacgcag gaacgacgga tgcccaactc ccgattgacg agggggggat tgtaaaagat
     4021 gaaatgcttg cgcccgcgcg gcgccccgtt gtcgtcgacc agacagaggg gttcgccggt
     4081 gagataacgg gcgaaatctc ccggattggc gatcgtggct gagcagcaga tgaaccgggg
     4141 ttgagagccg taaaaagcac agatgcgctt gaggcggcgc agtacattgg cgaagtgact
     4201 gccgaacacc ccgcgataat tatgaatttc gtcgatgaca atgtaacgga ggttttcgaa
     4261 gagcttgacc cagatggagt gatgaggcaa aatgccggta tggagcatgt cgggattggt
     4321 gacgacgatg tggccggagg tacgg
//
LOCUS       DAOPWU010000299         4341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_20367_length_4341_cov_4.804946, whole genome shotgun sequence.
ACCESSION   DAOPWU010000299 DAOPWU010000000
VERSION     DAOPWU010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20367_length_4341_cov_4.804946"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PKM23_12550"
     CDS             complement(<1..1120)
                     /locus_tag="PKM23_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNY92346.1"
                     /translation="MINNIIFNFDSTLIKTEGVELILQRALQRLDPKERSGRLEELGK
                     ISCLADIGETSVAEALHQCFGLTGVNRGDVEAVAAGIMEEVNPRVIESIQALQEAGKR
                     IFILSTGFDELVRPVTRALHVDDDHVFTNQLIYDFRGEVVGFNERNPLFLSAGKGFLV
                     EQLKNDGRLPGGTTVVGRSASDLAIRKNNVAQLFVYYLNTRIHEEIRRQADFVIERFD
                     QLLPLFFSEEEFSNERMQVYYQQNGAVEEEVKPNVLLLENIHDAAVQQMKRAGLEPRL
                     LKSALSIDELCSDAADVHVLGIRSQTRVTARAIACMENLMVIGAFCIGTNQVDLEAAA
                     AAGIPVFNAPYSNTRSVAELVVGEIIMLMRRIPEKSQAA"
     gene            complement(1222..1911)
                     /locus_tag="PKM23_12555"
     CDS             complement(1222..1911)
                     /locus_tag="PKM23_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNY92347.1"
                     /translation="MLRAIIFDFDGVVVDSEPIHLEAIKAALGDLGAELTPELYAQRY
                     LAYDDLRGFALFFADRGKELAEEELRAVVARKGAIYMEMIQAGKANFFPGVAELIREL
                     ARSTPLAIATGSLRHEVRALLESGGLLSCFRVVVSAEDVSRGKPDPESYLLALAELNR
                     VTGAVPAIAPGEALVIEDSRQGIASARRAGMKALAVSTSYPARELAEADLILPALAGL
                     QFSTLAELFPG"
     gene            complement(1920..2264)
                     /gene="hisI"
                     /locus_tag="PKM23_12560"
                     /pseudo
     CDS             complement(1920..2264)
                     /gene="hisI"
                     /locus_tag="PKM23_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            complement(2289..3047)
                     /gene="hisF"
                     /locus_tag="PKM23_12565"
     CDS             complement(2289..3047)
                     /gene="hisF"
                     /locus_tag="PKM23_12565"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNY92348.1"
                     /translation="MLAKRIIPCLDVKEGRVVKGIQFLQLRDAGDPVAQARYYDEQGA
                     DELVFLDITASSEKRNILLDVVARTAEQIFMPLSVGGGVRSLADAQMLLQRGADKVAV
                     NTAAIARPGLVSELAERFGSQAVVVAIDARRDPALGWRVYSHGGRIPLELETLAWAHR
                     VESLGAGEILLTSMDCDGTGEGYDLELTGAVSRALTIPVIASGGCGTPEHIHDVFALA
                     GADAALAASIFHYRRNSIPEVKRYLAGRQIPVRL"
     gene            complement(3051..3782)
                     /gene="hisA"
                     /locus_tag="PKM23_12570"
     CDS             complement(3051..3782)
                     /gene="hisA"
                     /locus_tag="PKM23_12570"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNY92349.1"
                     /translation="MLIIPAIDLMEGRCVRLKQGVQATRKIYADDPAAVAAQWEEEGA
                     TWIHVVDLDGAFGRTGANGRAITAILRRTGCAVELGGGIRTLEDIEAWLELGVARVIL
                     GTAAVLRPELAGEAVHHFGAGRIVVGVDARGERVAIRGWEEESAWQLADFVAAMETAG
                     VRRIIYTDIARDGEGSGPNLARLEAIARSTGMGIIASGGISRPEHLAALAGLALANIE
                     GAILGTALYEQELILADLIRICQGE"
     gene            complement(3793..>4341)
                     /gene="hisH"
                     /locus_tag="PKM23_12575"
     CDS             complement(3793..>4341)
                     /gene="hisH"
                     /locus_tag="PKM23_12575"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNY92350.1"
                     /translation="ALEHLGTEARVSSSSAEIIMADKLILPGVGAFGKALEAIDGLGL
                     REAICDFVASGRPFLGICLGMQLLFETSEESPGAAGLGLLPGRVRRFGPGLKVPHLGW
                     NALEQRQPSPLWKGIPPGSYFYFAHSYFVEPAEPEVIAGVTGYGGPVPAAIGSGMIHA
                     VQFHPEKSQQHGLQLLRNFITL"
BASE COUNT          797 a         1427 c         1357 g          760 t
ORIGIN      
        1 gggcggcctg gctcttctcc gggatgcgcc gcatcagcat gatgatctcc cccaccacca
       61 gctccgcgac gctgcgggta ttgctgtagg gtgcattgaa caccggaatg ccggcggcag
      121 ccgccgcttc gagatcgacc tgattggtgc cgatacaaaa ggccccgatc accatgagat
      181 tttccatgca ggcgatcgcc ctggcggtca ccctggtctg cgagcggatg cccagtacgt
      241 ggacatcggc cgcatccgaa cagagctcgt caatgctcag ggcgctcttg agcaggcgcg
      301 gctccagccc cgcccgcttc atctgctgca ccgctgcatc atggatgttt tccagcagca
      361 gcacattcgg cttgacctcc tcctccaccg ccccgttctg ctggtaataa acctgcatcc
      421 tttcgttgga gaactcctcc tctgagaaaa aaagcggcag gagctgatcg aagcgttcaa
      481 tgacgaaatc ggcctgacgg cggatctctt catggatccg ggtgttgagg taatagacga
      541 agagctgggc gacgttgttc ttgcggatcg ccaggtcgga agcgctgcgg ccgaccacag
      601 tagtacctcc gggcaggcgg ccgtcatttt taagctgctc gaccagaaag cccttgccgg
      661 cgctgaggaa gaggggattg cgctcgttga aaccgacgac ctccccacgg aaatcgtaga
      721 tgagctggtt ggtgaagacg tggtcgtcat ccacatgcag ggcccgcgtc accgggcgta
      781 ccagctcgtc gaaaccggtg ctgaggataa agatgcgctt gccggcctcc tggagggcct
      841 ggatgctctc gatgacccgg ggattgacct cttccatgat tcccgccgcc accgcctcga
      901 catccccccg gttgacgccg gtcaggccga agcactgatg caaggcctca gccacggagg
      961 tttcgccgat atctgcgagg caggagatct tgcccagctc ttcgagccgc ccgctgcgct
     1021 cttttggatc gagccgctgc agggcccgct ggaggatcag ttccactccc tcggttttga
     1081 tcagggtgga gtcaaaattg aaaataatgt tgttgatcat gctcttgtcg cgatgattgt
     1141 gcagggaaaa attttgctcg atatgaatat acaagttagt ctttcgggac ttcccaaaca
     1201 agtggaaaaa tggcctcgcg atcagcccgg gaaaagctcc gccagggtgg agaattgcag
     1261 ccctgccagt gcaggcagga tcaggtcggc ttctgccagc tcacgggccg gataggaggt
     1321 gctcactgcc agggccttca tgccggcgcg gcgtgcgctg gcaatcccct gcctcgaatc
     1381 ctcgatcacc agggcctccc ccggcgcgat ggccggaacc gcccccgtca cccggttgag
     1441 ctccgccagg gcgaggaggt agctctcggg atcgggcttg ccccggctca catcctccgc
     1501 cgagacgacc acgcggaagc aggagagtaa tccgccggac tcgagcaggg cgcgcacctc
     1561 atggcgcagg gagccggtgg cgatcgcgag gggcgtggat cgggccaatt cccggatcaa
     1621 ttccgcaact cccgggaaaa agttggcctt gcccgcctga atcatctcca tataaatcgc
     1681 ccccttgcgc gccacaaccg cccgcagttc ctcttccgcg agctccttgc cccggtcggc
     1741 gaagaagaga gcgaatccgc gcagatcatc ataagcgagg tagcgctgag cgtagagttc
     1801 cggggtgagt tcagcgccca aatcgccgag ggcggccttg atcgcctcga gatggatggg
     1861 ctcggaatcg accaccacgc cgtcgaaatc gaaaatgatc gccctcagca tgcctcctcc
     1921 tccagcttcc aggagaagca gctgcgtttg ccggtatggc aggtcgggcc gcagggttcg
     1981 gccatgacca gaatggcatc gcggtcgcag tcggcgtaaa gggcgcgcac gagaagaaaa
     2041 ttccccgaac tctccccctt ggtccagagg gtctggcgcg aacggctcca gaaggtgacc
     2101 ttgccgctct cgagggtgat gcggaggctt tcggcattca tatagccgag catgagcaca
     2161 tcatgcgcgg cggcgtcctg aatgatggcc gggaccagcc ccttctcatc gaatctgacg
     2221 caggcggtga attcggcggg agcgaggttt tccgtttctg acatggggct ctccttgcgg
     2281 gttattattc agagccggac cggaatctgc cgtccggcca ggtaacgctt gacttcagga
     2341 atcgaattgc ggcggtagtg gaagattgag gccgccaggg cggcatccgc cccggcgagg
     2401 gcgaagacgt catggatatg ctccggagta ccacagccgc ccgaggcgat gacggggatg
     2461 gtgagggccc ggctgaccgc cccggtgagt tcgagatcat agccctcgcc ggtgccgtcg
     2521 cagtccatcg aggtgagcag gatctccccg gccccgaggg attcgacccg gtgggcccag
     2581 gcgagggtct ccagctccag ggggatgcgc ccgccatggg aatagacccg ccagcccagc
     2641 gccggatcgc ggcgggcgtc gatggccacc accaccgcct ggctgccgaa acgctcggcc
     2701 agttcgctga ccagcccggg gcgggcaatc gccgctgtgt tgacggccac cttgtcggcc
     2761 ccccgctgca gcagcatctg ggcgtcggcg aggctgcgca cccctccgcc gacggaaagg
     2821 ggcatgaaga tctgctccgc cgtccgcgcc accacatcga ggaggatatt ccgcttttcg
     2881 gacgaggccg tgatatcgag aaaaaccagc tcgtcggccc cctgctcgtc gtaatagcgc
     2941 gcctgggcga cgggatctcc ggcatcccgc agctgcagga actggatgcc ctttacgacc
     3001 cgcccctcct tgacatcgag gcagggaatg atgcgtttgg ccagcatgtt ctactcccct
     3061 tgacagatcc ggatcaggtc agccaggatc agctcttgtt cgtagagggc ggtgccgagg
     3121 atggccccct cgatgttggc cagtgcaagc ccggcgaggg ccgccaggtg ttccggccgg
     3181 gagatgccgc cggaggcgat gatgcccatg cccgtcgagc gggcgatggc ctccagccgg
     3241 gcgagattgg ggccgctccc ctcgccgtcc cgggcgatat cggtatagat gatgcgccgg
     3301 accccggccg tctccatggc ggcgacgaaa tccgccagct gccaggccga ctcctcctcc
     3361 caccccctga tggcgacccg ctcgccccgg gcgtcgactc cgacgacaat tcgaccggcc
     3421 ccgaaatggt gcacagcctc gccggccagc tcgggccgca gcaccgcggc agtgcccaga
     3481 atcacccgcg ccacccccag ctccagccag gcctcgatat cctccagggt gcggatgccc
     3541 ccacccaatt cgacggcgca gccggtgcgc ctgaggatgg cagtgatggc gcggccgttg
     3601 gcgccggtac ggccgaaggc cccgtcgaga tcgacgacat gaatccaggt ggccccctcc
     3661 tcttcccact gcgctgccac cgcggcggga tcatcggcat agatcttccg ggtcgcctgc
     3721 accccctgct tgaggcgcac acagcgcccc tccatcaggt cgatggcggg tatgataagc
     3781 attttcttcc cttcacaagg tgataaagtt gcgcaaaagc tgcagtccgt gctgctggga
     3841 tttttccgga tgaaactgca cggcatggat catccccgac ccgatcgccg ccggcacggg
     3901 gccaccgtag ccggtgaccc cggcgatcac ctccggttcc gccggctcca caaaatagga
     3961 atgggcgaaa tagaaatagc tgcccggagg gatccccttc cacaggggtg agggctgtct
     4021 ctgttcgaga gcgttccagc cgagatgggg caccttgagc cccggcccga agcggcgcac
     4081 ccgcccgggc agcagcccca ggccggccgc ccccgggctc tcctcgctgg tttcgaaaag
     4141 cagctgcatc cccaggcaga tccccagaaa ggggcggccg ctcgcgacaa agtcgcagat
     4201 cgcctcgcgc aagccgagcc cgtcgatggc ttcgagggct ttgccgaaag ccccgacgcc
     4261 cggcaggatg agcttgtccg ccattataat ctctgcagat gaagagctca cccgcgcctc
     4321 ggtccccagg tgctcgaggg c
//
LOCUS       DAOPWU010000300         4312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_20545_length_4312_cov_5.238196, whole genome shotgun sequence.
ACCESSION   DAOPWU010000300 DAOPWU010000000
VERSION     DAOPWU010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20545_length_4312_cov_5.238196"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKM23_12580"
     CDS             complement(<1..548)
                     /locus_tag="PKM23_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNY92351.1"
                     /translation="MKVTINNKECWANPGETILTVAQREGIQIPTLCYLKGFTPTGSC
                     RVCVVEVEGQRNLIPSCAYPVTEGMVVLTNSPRVRRSRKTIVELLIANHPQDCLTCVR
                     NGNCELQDLAAQYGVRDVRYAGEIRGGKIDVASPSIERDPEKCILCGRCVRVCHEVQN
                     VGAIDFVRRGFKTAVAPAMDGSL"
     gene            complement(565..2562)
                     /locus_tag="PKM23_12585"
     CDS             complement(565..2562)
                     /locus_tag="PKM23_12585"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNY92352.1"
                     /translation="MNSYAQIFANCWHSSEKPCVHYIECINEGQKCHQSAESKKQLAA
                     FKSEILLQDHKEPVILIGMGTCGLANGANRVHAAVLSELERLNLEIKVVPTGCVGYCS
                     KEVIVDIKIPGRSRICYCQVKPEDVPMLLQKTLIDKEVVEEKLLGIYGSTNGETGWKE
                     LPLLHQVPFFAKQKKLVLENCGVIDPESIDQYLARGGYVGLSRAINKMTPAEVVKEIL
                     KSGLRGRGGGGFPTGKKWEFALNQPSEQKYIVCNADEGDPGAFMDRAVMESDPHRVIE
                     GMIIGAYAIGASEGYIYCRAEYPLAIKRLEKTIADAKAHGLLGQNILGSGFNFNLKIK
                     KGAGAFVCGEETALLHSIEGKRGMPRPRPPFPAVSGLFGKPTVINNVETFANVPNIMR
                     DGADKYAAIGTEGSKGTKIFALSGKITNTGLVEVPMGVTLREVVYDIGAGCPNGKKFK
                     AVQIGGPSGGALPEQVIDTQIDYENLKKVGAMMGSGGLVVMDETTCMVDVAKYFMTFI
                     QSESCGKCIPCREGTKRLLEILERLTQSYRSEKSPDEALLRFQGMVYLERLSEVIQNT
                     SLCGLGQTASNPVMSTLRYFRDEYEAHLYDRKCPSGHCRELLTYHIDTDKCTGCTVCA
                     RKCPQAAILGEKGKAHYIIDEKCIRCDQCRANCKFDAIFVE"
     gene            complement(2573..3034)
                     /gene="nuoE"
                     /locus_tag="PKM23_12590"
     CDS             complement(2573..3034)
                     /gene="nuoE"
                     /locus_tag="PKM23_12590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015849787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNY92353.1"
                     /translation="MEIQEVYAKYPQNDPSILIPILQDIQEVCGYLSEDDLEQVAQHT
                     RLPVSRVFGVATFYNQFRLRPLGKHVIRVCRGTACHVKGSFDILTTLENELGIKAGQT
                     TKDLQFSIETVACIGACSIAPVITIDDEFHGGLTVKAVQKLIAALKKTNNE"
     gene            complement(3064..3438)
                     /locus_tag="PKM23_12595"
     CDS             complement(3064..3438)
                     /locus_tag="PKM23_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY92354.1"
                     /translation="MNCLIVANPQKSITRELEGFLRTNAIATLLVEDPVQAIEQVQRQ
                     HFDLIVLDARAHGLKIDQAIRLLKGCDPSVRIIVRTDENTRNLEGRVRREQVFYYHVD
                     SLGMHDLQLAITAALGIETLKH"
     gene            complement(3681..>4312)
                     /locus_tag="PKM23_12600"
     CDS             complement(3681..>4312)
                     /locus_tag="PKM23_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNY92355.1"
                     /translation="ISLNVQAKILRAIQEREIRRIGSAQTIPVDVRVIAATNIDLNAA
                     VNTGQFREDLFYRLSVIPIHLPPLRERPEDIDTLVDHFIQKHNKRRRQNPIDKVAGPV
                     RDLLLHYPWPGNIRELENTIERAAVIEESTELTLQSLPPHLQLYGEKAGEAAASELLS
                     LAEVEKEHIRKIMQNEENNISRAARILGIDRKTLYDKIRRYGLSYSGSS"
BASE COUNT          838 a         1132 c         1327 g         1015 t
ORIGIN      
        1 agactgccat ccatggccgg ggcgacagcg gtcttgaagc cgcggcggac gaaatcgatc
       61 gctccgacgt tctgaacctc atggcagacg cgcacgcagc ggccgcagag gatgcatttc
      121 tcgggatcac gttcgattga aggactggcg acgtcgatct tgcccccacg gatttcgcct
      181 gcgtagcgca catcgcggac gccatactgc gccgccagat cctgcagttc gcagttgccg
      241 ttgcgcacgc aggtgaggca gtcctgggga tggttggcga tgagcagctc gacgatggtc
      301 ttgcgggaac ggcgcacgcg gggtgaattg gtcagaacca ccatcccctc tgtcaccgga
      361 taggcacagg agggaatgag gttgcgctgg ccttcaacct cgaccacaca gacccggcag
      421 gagccggtcg gagtgaagcc cttgagatag cagagggtcg gaatctggat gccctcgcgc
      481 tgtgcgaccg tgagaatggt ttcgcccggg ttggcccagc actctttatt attaatcgtt
      541 accttcatgt tggtcctctt ggttttactc tacaaagatc gcatcgaatt tgcaattggc
      601 gcggcactga tcgcagcgga tgcacttctc gtcaatgatg taatgggcct tgcccttctc
      661 gcccagaatg gcggcctggg gacacttgcg tgcacagact gtgcagcccg tgcatttgtc
      721 ggtgtcgata tggtaggtca ggagctcgcg gcagtggccg gaggggcatt tgcgatcgta
      781 gagatgggct tcatactcat cgcggaaata acgcagagtg ctcatgaccg ggttagaggc
      841 ggtctgcccc aatccgcaga gggaggtatt ctggatcacc tcggagaggc gttccaggta
      901 gaccataccc tggaaacgca gcagggcctc gtccggggat ttttcgctgc ggtagctttg
      961 ggtcagccgt tcgaggatct cgagcagacg tttggtgccc tcacggcagg gaatgcactt
     1021 gccgcaggat tcggactgaa tgaaggtcat gaaatacttg gcgacgtcga ccatgcaggt
     1081 cgtctcgtcc atgaccacca gtccgccgga gcccatcatg gcgccgacct ttttgaggtt
     1141 ttcatagtcg atctgggtat cgatgacctg ttccggcagg gcgccgccgg agggtccgcc
     1201 gatctgcacc gccttgaatt ttttaccgtt ggggcagccg gcgccgatat catagaccac
     1261 ctcgcgcagg gtcacgccca tgggaacctc caccaggccg gtattggtga tcttgccgga
     1321 gagggcgaaa atcttggtac ccttggaacc ctcggtgccg atggcggcat atttgtcggc
     1381 gccatcgcgc atgatattgg gcacattggc gaaggtctcg acgttgttga tcaccgtcgg
     1441 cttgccgaaa agaccggaga ccgccgggaa gggcgggcgt ggccgcggca tgccgcgttt
     1501 accttcaatg gagtggagca gggcggtctc ttcgccgcag acgaaagcgc cggctccctt
     1561 cttgatcttg agattgaagt tgaagccgct gccgagaata ttctggccga gcagtccatg
     1621 ggccttggcg tcggcgatgg ttttctccag ccgcttgatg gcgagggggt attcggcacg
     1681 gcagtagata tagccctcgc tggccccgat ggcgtaggcc ccgatgatca taccctcgat
     1741 gacgcggtgg ggatcgcttt ccatcacagc gcggtccata aaggcgcccg gatcgccctc
     1801 gtcggcgttg caaacgatgt atttttgttc ggagggctgg ttgagggcga attcccactt
     1861 ctttccggtc gggaagccgc cgccgccgcg tccgcgcaga cccgatttga ggatctcctt
     1921 gaccacttca gccggggtca ttttgttgat ggcgcgggag aggccgacgt agccgccgcg
     1981 ggcgagatac tggtcaatgc tctcgggatc gatgacgccg cagttttcaa gtaccagttt
     2041 cttctgcttg gcgaaaaagg gcacctgatg gagaaggggc agctctttcc agccggtttc
     2101 gccgttggtg gaaccgtaga tgccgaggag cttttcctca accacctctt tgtcgatcag
     2161 ggtcttctgc agcagcatgg ggacatcttc cggcttgacc tggcagtaac agatgcggga
     2221 gcggcccggg atcttgatat caacgatcac ctctttggag cagtagccga cgcagccggt
     2281 gggcaccacc ttgatctcga ggttgagcct ttccagttcg ctcaggaccg ctgcatgcac
     2341 cctgttggcg ccgttggcaa gaccgcaggt gcccatgcca atgagaataa caggttcctt
     2401 gtggtcctgg agcaggattt cggacttgaa ggcggcaagc tgtttcttgc tctccgcgga
     2461 ctgatggcat ttttgccctt cgttgatgca ttcgatgtag tggacgcagg gtttttcact
     2521 gctgtgccag cagttggcaa atatctgggc atatgaattc attacggcct ctttattcat
     2581 tgttggtctt tttcagagca gcgatcagct tttgcacagc cttgacggtc aatccgccgt
     2641 ggaattcgtc atcgatggta atcaccggag cgatgctgca ggcgccgatg caggcgaccg
     2701 tttcgatgga aaactggagg tctttggtgg tctggccggc tttgatgccc aattcgtttt
     2761 cgagcgtggt caggatgtcg aacgagccct tgacgtggca ggcggtgccg cggcagacgc
     2821 ggataacgtg cttgcccagg gggcgcagcc tgaactggtt gtaaaaggtc gccacaccga
     2881 agacgcggct gaccggcagc cgggtgtgct gtgcgacttg ctccagatcg tcctcggaga
     2941 gatagccgca cacctcctga atatcctgca ggatcggaat caggatcgag ggatcatttt
     3001 ggggatactt tgcgtagacc tcttgaattt ccatgctgtc ctcttgcaat atgactttgt
     3061 tgattagtgt ttgagcgtct cgatgcccag cgcggcggtg atggcgagct ggagatcgtg
     3121 catgcctagt gaatccacat gataataaaa aacctgttct ctgcgcaccc tcccctcgag
     3181 gttgcgcgtg ttctcatccg tgcgcacgat aatgcgtaca gagggatcgc atcccttaag
     3241 cagccggatg gcctggtcga ttttcaggcc atgggcccgg gcgtccagga cgatcagatc
     3301 gaagtgctgc cgctgcacct gttcaatggc ctggacggga tcctcgacca gaagcgtggc
     3361 gatggcattg gtgcgaagaa aaccttccag ttcccgggtg atactttttt gggggttggc
     3421 cacaatgaga cagttcatag catcaaacca tataatactt cttttatctg aatatcttca
     3481 aatagcgtgc cagaggcgcc aattcgacct gctgatgttt atctttgatt attttgaaat
     3541 tatactgccc gatcacgtct gaaaatgagc cgtattctgc attctctcat ttatgagaga
     3601 atattctgtg gtgaaactcc acaccagcgc gtcgtagtaa agagggcaaa tccaggaata
     3661 aagaataaag cgtgagcagt tcagctggat ccagaatagg agaggccgta gcggcggatt
     3721 ttgtcgtaga gggtcttgcg gtcgatgccg agaattcttg cggcccggct gatattgttc
     3781 tcctcgtttt gcatgatttt gcggatatgc tccttttcga cctctgcgag ggaaagaagc
     3841 tcggaggcgg ctgcctcacc ggccttttcg ccgtagagct ggagatgcgg gggcaggctc
     3901 tgcaaggtga gttccgtgct ctcctcaatg acggcggctc gttcgatcgt gttctccagc
     3961 tcgcggatat tgccgggcca gggatagtgc agcaaaagat cgcggacggg accagcgact
     4021 ttatcgattg gattctgccg gcggcgctta ttgtgttttt ggatgaagtg atcaactaaa
     4081 gtatcgatat cttcgggtct ttcgcgcaac ggaggaagat gaattggaat gacgctcagc
     4141 cggtagaata ggtcctcgcg aaactgtccg gtgttcacag cggcgttgag atcgatattg
     4201 gtcgcggcga tgacgcgcac atccaccggg atggtctgtg cggagccaat tctgcggatc
     4261 tcgcgttcct ggatggcgcg caggatcttg gcctggacgt tgagggagat gt
//
LOCUS       DAOPWU010000301         4303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_20610_length_4303_cov_3.374058, whole genome shotgun sequence.
ACCESSION   DAOPWU010000301 DAOPWU010000000
VERSION     DAOPWU010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20610_length_4303_cov_3.374058"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /locus_tag="PKM23_12605"
     CDS             <1..1739
                     /locus_tag="PKM23_12605"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNY92356.1"
                     /translation="PGWLEEVLAHALEDFEKACTRWRELYRTALAQAKAQDKVIRDAT
                     RPQAERIRAERLRREAESQLKLLTEIENVVQSDFYVYRYFASEGFLPGYSFPRLPLSA
                     YVPGRRLKQHDEFLSRPRFLAISEFGPRAFIYHEGSRYDINRVLLTMQGDVLETAAAK
                     LCEHCGYLHPIQAGVGPDLCENCHNPLDLPLESLLRLQNVSTRRRDRITSDEEERMRL
                     GYELLTAVRFQDQDGRQAYIQGEIVADGKPLARVKYGHTATIWRINLGWKRRKNRAQS
                     GFLLDIDKGYWEKNENIPADEDEDLTNRVQRVRPFVEDRRNSLILQMMDDLDIGQMAS
                     LQAALKSAIQIEYQLEDQELAVEPLPDTDERKFILLYEAAEGGAGVLRRLLTDPGAFG
                     AVAARALELCHFDPATGQDLLKAPRAKENCEAACYDCLMSYGNQPDHLLLDRKQIRDL
                     LMLMAGAEVKLSPLAASREQHLRNLASLAGSELERQWLRLLQELNLRLPSHAQYLFEA
                     CHTRPDFYYQEHYAAVYIDGPHHEYPERQQRDHEQETALADRGITVIRFGHADDWRKI
                     LGQYPYIFGSMA"
     gene            1736..>4303
                     /locus_tag="PKM23_12610"
     CDS             1736..>4303
                     /locus_tag="PKM23_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNY92357.1"
                     /translation="MSYAVGALVKARGREWVVLPSSDDALLIVRPLGGADDEITGILT
                     ALENVQPAQFDLPDPSQLGDYRSCHLLRDALRIGFRSSAGPFRSFGKIAVEPRPYQLT
                     PLLMALKLDPVRLLIADDVGIGKTIEACLVARELLDRGEIERIAVLCPPQLAEQWQNE
                     LEEKFHIQAELVLTHTAGRLERECRPGESLFEHYPFTVVSTDFIKSDRRRDDFLRSCP
                     GFVIVDEAHTCAFASEHNRGQHQRHQLIQGLSRNPSRHLVLVTATPHSGKEDAFRSLL
                     GLLDPGFRDLPENLAGAENESHRRRLAQHFIQRQRGNIKAFLGEETRFPERALLEPEP
                     TYKLSEPYRQFFGQVIAYARETVAEPEKMTFRQRVAWWSALALLRALASSPAAAAATL
                     RNRAAAADTETIQEADEIGRRTVLDLVVEEAAESIDVTPGSDIGELAGDEQKNRDRLL
                     RMARTAESLQGAGDNKLQGAIQLLRQTLADGYHPIIFCRFIPTVHYLAAELHSALPKE
                     IEIAAITGDIPPAERETRIAQLGKAEKRILVCTDALSEGINLQEHFDAVMHYDLSWNP
                     TRHEQREGRVDRFGQPREVVRVITYYGIDNQIDGIVLDVLLRKHRQIRNSLGISIPVP
                     ARSDQLIEAIFEGLLLRAHPAGGDQLLLPGFEEYLKPQRDTIARQYDELYKREQVSRT
                     LFAQHTIKVEEVHDELRQVRAAIGGMSDVAGFTQSAVRALGGTVSENSCCTFDLRETP
                     PALRDIIGETAFKARFGLPVPEGVRYLNRTHPFVDSLASWLLDTALDPIETGVARRCG
                     VIRTSRVQRRTALILARCRYHLITIRDGREKTLLAEEAQILGFAGAPDEAVWLEKEAV
                     "
BASE COUNT          836 a         1449 c         1231 g          787 t
ORIGIN      
        1 atcccgggtg gctggaagag gtgctcgccc acgctctcga ggatttcgaa aaagcctgca
       61 cgcgttggcg cgagctctac cgcacagccc tcgcccaggc caaagcccag gacaaggtca
      121 tccgcgatgc gaccaggccg caggcggagc gcatccgcgc cgagcgcctg cgccgcgagg
      181 cggaatcgca gctcaagctt ctcaccgaaa tcgaaaacgt cgtgcaatcc gatttctacg
      241 tctatcgcta tttcgccagt gagggatttc tgccgggtta cagttttccg cgccttcccc
      301 tctcggccta tgtgcccggc cgccgcctca agcagcacga tgaatttctc tcccggcccc
      361 gcttcctcgc catctccgag ttcggtccgc gcgctttcat ctatcatgaa ggctcacgct
      421 acgatatcaa ccgcgtcctt ctcaccatgc agggcgatgt gctggagacc gctgccgcca
      481 aactgtgcga gcactgcggc taccttcatc ccattcaggc tggggtcggc cctgatctct
      541 gcgagaactg ccataatccc ctggacctgc cgctcgaatc gctcctgcgc ctgcaaaatg
      601 tctccacccg ccggcgcgac cgcatcacct ctgatgaaga ggagcggatg cggctgggct
      661 atgaactgct gaccgcggtg cgtttccagg accaggatgg ccggcaggct tatattcagg
      721 gcgaaatcgt cgcggacggc aagccgctgg cgagagtcaa atatggtcat acggccacca
      781 tctggcgcat caatctgggc tggaagcggc gcaaaaatcg cgcccagtcc ggctttctcc
      841 tcgatatcga caaaggctat tgggagaaaa acgaaaacat cccggcggat gaggatgaag
      901 atctcacaaa ccgggtccag cgggtgcggc cttttgtgga agaccggcgc aatagcttga
      961 ttttacagat gatggatgat cttgacatcg gtcaaatggc ctctctgcaa gccgccctga
     1021 aatcagccat ccagattgaa taccagctgg aggaccagga actggcggtc gagcctttgc
     1081 ccgataccga tgagcgcaaa ttcatactgc tatacgaagc cgccgagggc ggcgccggcg
     1141 tgctgcggcg tctgctcacc gatcccgggg catttggggc ggtcgcagcc agagccctgg
     1201 aattatgcca ctttgatccg gcgacggggc aggatctgct caaagcaccg cgcgccaaag
     1261 agaactgcga agcggcctgc tacgactgcc tgatgagcta tggcaatcag ccggatcacc
     1321 tcctcctcga tcgcaagcag atccgcgacc tgctcatgct aatggccggg gccgaagtga
     1381 aactgtctcc gctcgccgcc agccgcgaac agcatctgcg caatctggca agcctggccg
     1441 gctcggagct ggaacgccaa tggctgcggc tcctccagga gctcaacttg cgcctgcctt
     1501 cccatgccca atacctgttc gaagcgtgcc acacccgccc cgacttctat tatcaggagc
     1561 actatgcggc cgtctatatc gatggccccc atcatgagta cccggagcga cagcagcgcg
     1621 accatgaaca ggagacggcc ctggccgacc gcggcatcac cgtgatccgt ttcggccatg
     1681 ccgacgattg gcggaaaatt ctcggacaat atccctatat tttcgggagt atggcatgag
     1741 ctacgccgtc ggcgcattgg tcaaagcgcg cggccgggaa tgggtggttc tgcccagctc
     1801 ggatgacgcc ctgctcatag tgcggcccct cggcggcgcc gacgatgaga tcaccggcat
     1861 tctcacggcc ctggaaaatg tccagcccgc ccaattcgat ctgcccgatc cttcccagct
     1921 cggcgattac cgctcctgcc acctgctccg ggacgccctg cgcatcggct tccgctccag
     1981 cgccggcccg ttccgctcct ttggcaagat cgccgtcgag ccgcgtccct atcagctcac
     2041 gcccctgctt atggccctca aactcgatcc cgtgcgcctg ctcattgccg atgatgtcgg
     2101 catcggcaag accatcgagg cctgcctcgt cgcccgcgag ctgctcgatc gcggtgaaat
     2161 cgaacgcatt gccgtcctct gcccgccgca gctggccgaa cagtggcaga acgaactgga
     2221 agagaaattc catattcagg ccgagctggt gctgacccat acagccggcc ggctggagag
     2281 agagtgccgc ccgggcgaat ccctctttga gcattatccc tttaccgtgg tctcgaccga
     2341 tttcatcaaa tccgatcgcc gccgcgatga tttcctgcgc agctgccccg ggtttgtcat
     2401 cgtcgatgag gcccacacct gcgcctttgc cagcgagcat aatcgcggcc agcaccagcg
     2461 ccatcaactg atccagggtc tcagcaggaa tccatcccgc catctggttc tggtcaccgc
     2521 cacgcctcac agcggcaagg aggatgcctt ccgctccctg ctgggattgc tggatcccgg
     2581 ttttcgtgat cttccggaga atttggcagg agcggaaaat gaatcccatc gccgccggct
     2641 tgcgcagcat tttatccagc gccagcgcgg caacatcaag gcctttttgg gcgaggaaac
     2701 ccgcttcccg gaacgcgccc tgctcgagcc cgaaccgacc tacaagcttt ccgaacccta
     2761 ccggcagttc ttcggccagg tgatcgccta tgcccgtgaa accgtagctg aacctgaaaa
     2821 aatgaccttt cgccagcgcg tcgcctggtg gtcggccctg gccctgctgc gcgcccttgc
     2881 ctccagcccg gcggcagcag cagctaccct gcgcaaccgc gccgccgcag cggataccga
     2941 aaccatccag gaggcggatg agatcggccg ccgcacggtt ctcgatctgg tggtcgaaga
     3001 ggccgccgaa tccatcgacg tcactccagg cagcgacatc ggcgaactgg ccggggacga
     3061 gcagaagaac cgcgaccgcc tcctgcgcat ggcgcgaaca gccgagagcc tgcaaggagc
     3121 cggggacaac aagctccagg gcgccattca gctgctcagg cagaccctgg ctgacggcta
     3181 ccatcccatc atcttctgcc gcttcatccc caccgtccat tatctggctg cagagctgca
     3241 cagcgccctg cccaaagaga tcgagatcgc ggccatcacc ggcgacatcc cgccggcgga
     3301 gcgtgagacc cgcatcgccc aactgggtaa agcggaaaaa cgcatcctgg tttgtaccga
     3361 cgccctcagc gagggcatca acttgcagga acattttgac gcggtgatgc actacgatct
     3421 ctcctggaac cccacccgcc acgaacaacg cgaaggccgg gtggaccgct tcggccagcc
     3481 gcgcgaggtg gtgagggtga tcacctatta cggcatcgac aaccagatcg acggtatcgt
     3541 cctcgatgtg ctgctgcgca agcaccggca gatccgcaac tccctgggca tctcgatccc
     3601 ggtgccagcc cgttccgacc agctcatcga ggccattttt gagggactct tgctgcgcgc
     3661 ccacccggct ggcggcgatc agcttttact gcccggtttt gaggagtacc tcaagcccca
     3721 acgcgacact atcgcccgtc aatacgacga gctctacaag cgcgaacagg tctcgcggac
     3781 gctcttcgcc cagcatacca tcaaggtcga agaggtgcat gacgagctgc gccaggtgcg
     3841 cgcggctata ggcggcatga gcgacgtggc cggattcacc caatcggctg tccgtgctct
     3901 gggcgggacg gtctctgaaa acagctgttg tacattcgac ctgcgcgaaa ccccaccggc
     3961 cttgcgcgac atcatcggcg aaaccgcttt caaggcccgc tttggactgc cggtcccgga
     4021 gggcgtgcgc tatctcaacc gcacccatcc cttcgtcgac agcctggcct cctggctgct
     4081 cgacaccgcc ctggatccca ttgaaacggg tgtggccagg cgctgcgggg tcatccgcac
     4141 cagccgggtg cagcggcgca cggcactgat cctggcccgc tgccgttatc atctgatcac
     4201 gattagggat ggccgggaaa agaccctgct ggctgaggag gcgcagattc tcggctttgc
     4261 cggcgccccc gatgaggcgg tttggctcga aaaagaggcg gtc
//
LOCUS       DAOPWU010000302         4262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_20883_length_4262_cov_4.463275, whole genome shotgun sequence.
ACCESSION   DAOPWU010000302 DAOPWU010000000
VERSION     DAOPWU010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20883_length_4262_cov_4.463275"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="PKM23_12615"
     CDS             complement(<1..1517)
                     /locus_tag="PKM23_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92358.1"
                     /translation="MKPWMLVVIAGFACALFAQEPGWPPRSSGEFFYRYEARSGDPIA
                     ENPGEWSSSTTPPGVPFLESGTLGMEVGMNRYWSLHPDLLGVLDHARGWTMEVRLRVI
                     AQSGRSGAIAMAVDDEPGQPGVLSGLQIHSDSTQFCFNGAVVDTRSNSDGFHTFRLAE
                     EANSRTVHAWRDGRYLGSASYPYYMISLSHFLLVGSWSGRIAGTAEIDYIRWEPSGAY
                     APAAALLVISESDGSTQVSEKGPGSDSFAVVLGHAPAADVTVMIAPGFGSSRAEEADL
                     GAGSGGAITLTFTPANWNIPQNVIVTAVDDTLREWFHTSVIGCRTASSDPDFDGLEEL
                     VTLFVMDNDEVIRLVESGEGTEVREEGSTTDFYRFRFGQLPDQTVQVALHFDPRRLRV
                     NGGTVSPQVITLSPAEAQNPLPIEVAAFDNTDRDLLLHARIRHLLTSADPLFNGAAVP
                     DLAVRIHENDTQHLQPDSFTIPLVNFDQADRQVVVHRPPGQYLGHPTTVLLEDSTT"
     gene            complement(1665..3053)
                     /locus_tag="PKM23_12620"
     CDS             complement(1665..3053)
                     /locus_tag="PKM23_12620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNY92359.1"
                     /translation="MTGPAGKIKARYHEEGGYRAVMDMALPLIFSTGAWTVQTFIDRV
                     FLTWYSPEALAAAAPAGMANFTLFSLLIGIASYVNTFVAQYHGAGRPERIGRSVWQGI
                     YFSLVALLIAVVIWPLADDLFRFFGHAPEVQEMEVVYFRILAVGAPAVVISNAISGLF
                     SGLGKTWIIFWVNFAATLINMVLDYLLIFGKMGLPGMGIAGAAWATVAAGFFPALVFA
                     GIMILPRYARTYGTLRSWRFDRALFRRLLRFGTPNGLQVFLEVLAFTIFLVIAGKIGT
                     IELAATTLAFNINNLAFAPMLGMMTAVSALVGQSLGRERPELAEKLTWSALHICGAFY
                     VTLGLAYAAVPQLFLWPFLRNAQSAEFLEISRVGVNLLRFVAVYSIFDAMNMIFSAAI
                     KGAGDTRYVAVTTIRLSWLVLVLPSIVWWFFARGSIYGLWVFITLYVIGLGLFFLRRF
                     LHGPWRTMRVID"
     gene            complement(3050..>4262)
                     /locus_tag="PKM23_12625"
     CDS             complement(3050..>4262)
                     /locus_tag="PKM23_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNY92360.1"
                     /translation="AKRELTIQLVRILFPGAGLLVLSSWCLGILNSHRKFFLSYAAPI
                     GWNAVMIAALVIWGPRQQQFDLAPTIAWASVAGSGLQLLVQLPMVLRLAKHLRIRIDL
                     KMAEVRTVVKNFFPVFFGRGVIQISSYVDELLASLLPTGAFAALSYAQTLYLLPVSLF
                     GMSVSAAELPAMSSVVGDEKEISGYLQKRLNSGLRRIAFFIIPSAIAFLALGDVVVAA
                     IYQTGRFGHREVIHVWGVLAGATVGLMAQALGRLYSSTYYALRDTRTPLRFAVVRVLF
                     TCTMGYLFALRLPGLIGLDPRWGVACLTVSSSLAAWVEFILLRHTLNRRIGRTGLQGG
                     RTFRLFLSAALAAAAAWGMRSLLPPLHPILLAALLLSLYGGLYVGSTLLLRAAEIAPV
                     LARLQRRRHTP"
BASE COUNT          905 a         1253 c         1453 g          651 t
ORIGIN      
        1 gtggtgctgt cctcgagcag caccgtggtg ggatggccca ggtactgccc gggcggccgg
       61 tggacgacca cctggcggtc agcctgatca aaatttacca gtggaatggt aaagctgtcc
      121 ggctggaggt gctgggtatc gttctcgtgg atccgcaccg ccagatcggg gacagcggcg
      181 ccgttgaaga gggggtcggc gctggtcaaa agatgacgga tgcgcgcgtg cagcagcagg
      241 tcccggtcgg tgttatcaaa ggcggctacc tcgatgggga ggggattctg tgcctccgcc
      301 ggtgagaggg tgatgacctg gggagaaacc gtgccgccgt tgacccgcag ccgccgcgga
      361 tcgaaatgga gggccacctg caccgtctgg tcgggcagct gaccgaagcg gaaacggtag
      421 aaatcggtgg tgctcccctc ttcccggacc tccgtaccct cgccgctctc caccagccgg
      481 atcacctcat cgttatccat cacaaacagg gtcaccagct cctcgaggcc gtcgaaatcg
      541 ggatcgctgc tcgccgtccg gcagccgatg acgctggtgt gaaaccactc ccgcagggtg
      601 tcatcgacgg cggtgacgat gacattttgc ggtatgttcc agttcgccgg cgtaaaggtg
      661 agagtaatgg cgccgccgga gccggcgcca aggtcggcct cctcagcccg gctggagccg
      721 aagccgggtg cgatcatgac ggtgacatcg gctgcagggg cgtgcccgag gacgacggca
      781 aagctgtcgc tgcccggtcc cttttcgctc acctgtgtgg agccatcgct ctcgctgatg
      841 accagcagcg ctgcggccgg ggcataagcg ccggaaggct cccagcggat ataatcgatc
      901 tcggccgtgc cggcgatgcg tccgctccag gagccgacga ggaggaaatg gctgaggctg
      961 atcatataat agggatagct ggccgatccc agatagcggc cgtcgcgcca ggcgtggacc
     1021 gtgcggctgt ttgcctcttc ggcgaggcga aaggtgtgaa aaccgtcgct gttggagcgg
     1081 gtgtcgacga ccgcgccgtt gaagcagaat tgggtgctgt cgctgtgaat ctgcaggccg
     1141 ctgagcacgc cgggctggcc cggttcgtca tccacagcca tcgcgatggc gccgctgcgg
     1201 ccgctctgcg cgatcacacg cagccgcacc tccatggtcc aacccctggc gtggtcgagc
     1261 acccccagca gatccgggtg cagcgaccag tagcggttca tccccacctc catgccgagg
     1321 gtgccggatt ccagaaaggg cacccccgga ggcgtcgtcg aggaagacca ctcgccggga
     1381 ttctcggcga tgggatcgcc cgagcgggct tcatagcggt agaagaactc cccggaagag
     1441 cggggtggcc atccgggttc ttgtgcaaag agggcgcagg caaaaccggc gataacgaca
     1501 agcatccagg gcttcatgtt cgacctcatg gcatgactgg ttttttttgt aataaaaatt
     1561 actaaaaatt ggcgggaaaa caaggacttt tttggcagga agcgccggaa ggcagcttta
     1621 ttacaacccg gaggggtgag aaaatgggga gaagcttgta ttccctaatc gataacccgc
     1681 atcgtccgcc agggaccgtg cagaaaacgg cgcagaaaaa agagccccag gccgatcaca
     1741 tagagggtga tgaaaaccca cagcccgtag atcgaccccc tggcgaagaa ccaccagact
     1801 atggagggca ggaccagcac cagccagctg agccggatgg tcgtgaccgc cacgtagcgg
     1861 gtgtcgcctg cccccttgat cgcggcggag aaaatcatgt tcatggcgtc gaagatgctg
     1921 taaaccgcga cgaagcgcag cagattgacg ccgacccggc tgatctcgag aaattcggcc
     1981 gactgggcgt tgcggagaaa aggccataaa aagagctgcg gcaccgctgc ataggcgagg
     2041 ccgagagtga cataaaaggc cccgcagatg tgcagggcgg accaggtcag cttttcggcg
     2101 agctcgggcc gctcccggcc gagtgattgg ccgacgaggg cgctgaccgc agtcatcatg
     2161 cccagcatcg gagcgaaggc gagattgttg atattgaaag ccagggtcgt tgctgccagc
     2221 tcgatcgtgc cgatcttgcc cgcgatgacc agaaatatgg tgaaggccag gacttcgaga
     2281 aaaacctgca agccgttcgg ggtgccgaag cgcaggagac ggcggaagag ggcgcggtcg
     2341 aagcgccagc tgcgcagggt gccataggtg cgcgcataac ggggcaggat cataatcccc
     2401 gcaaagacga gcgccgggaa gaaacccgcc gccaccgtcg cccaggccgc gccggcaata
     2461 cccattcctg gcagtcccat tttgccgaag atgagcagat agtccagcac catgttgatg
     2521 agcgtggcgg caaaattgac ccagaagatg atccaggtct tgcccaggcc gctgaaaagg
     2581 cccgagatcg cgttcgaaat caccaccgcc ggggcgccta ccgcgaggat gcggaagtag
     2641 accacctcca tctcctgcac ctccggcgca tggccgaaga accggaagag atcgtcggcg
     2701 agcggccaga tcacgacggc gataaggagg gcgacaaggc tgaaataaat cccctgccag
     2761 accgagcggc cgatgcgttc ggggcggccc gcgccatgat actgggcgac gaaggtattg
     2821 acatagctgg cgatgccgat gagcaggctg aagagggtga aattggccat gcccgcgggg
     2881 gcggcggcgg ccagggcctc gggggaatac caggtcagaa agacgcggtc gatgaaggtc
     2941 tgcaccgtcc aggctccggt gctgaagatc aggggcaggg ccatgtccat caccgcccgg
     3001 tagcctccct cctcatggta gcgcgccttt atttttcccg ccgggcctgt cacggagtat
     3061 ggcgcctgcg ctgcagacgc gccagcaccg gggcgatctc cgcagcccgg agcagcaggg
     3121 tgctgccgac atagagcccg ccatagaggg agaggagcag tgctgccagc aggatcggat
     3181 ggagcggcgg cagcaggctg cgcatacccc aggcggcggc ggccgccagg gctgcgctga
     3241 ggaaaagccg aaatgtgcgg ccgccctgca gtccggtgcg gccgatgcgg cggttaaggg
     3301 tgtggcgcag gagaataaat tccacccagg cggccaggct cgaggagacg gtcagacagg
     3361 cgacccccca gcgtggatcc aggccgatca gcccgggcag gcgcagggcg aagagataac
     3421 ccatggtgca ggtgaacagg acgcgcacga cggcgaagcg caggggggtg cgggtatcgc
     3481 gcagggcata ataggtggag gagtagaggc ggccgagggc ctgggccatc agtccgactg
     3541 tggctccggc gaggaccccc cagacatgga tcacctcccg gtggccgaaa cgtccggtct
     3601 gataaatagc cgcgaccacg acatcaccaa gagcgagaaa ggcgatcgcc gaggggataa
     3661 taaaaaaggc gatacggcgc aggccgctgt tcagccgctt ctgcagatag cccgagatct
     3721 ccttttcgtc gcccaccaca ctcgacatcg ccggcagttc agccgctgag accgacatcc
     3781 cgaagaggct gaccggcagg aggtagaggg tctgggcgta gctcagggcg gcaaaggcgc
     3841 cggtgggcag caggctggcc aggagttcat cgacatagct gctgatctgg atcacaccgc
     3901 ggccgaaaaa aaccggaaaa aaattcttca ccaccgtgcg cacctcggcc atcttgaggt
     3961 cgatgcggat gcgcagatgc ttggccaggc gcagcaccat gggcagctgc accagcagct
     4021 gcaggccgct gccggccacg gaggcccagg cgatggtcgg ggccaggtcg aactgctgct
     4081 ggcgcggccc ccagatcacc agtgcggcga tcatcaccgc attccacccg atgggcgcag
     4141 cgtaggagag gaagaatttg cggtggctgt tgaggatgcc caggcaccag gaggagagaa
     4201 cgagcaggcc tgcgcccggg aagaggatgc gcaccagctg gatggtcaat tcgcgcttgg
     4261 ct
//
LOCUS       DAOPWU010000303         4243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21021_length_4243_cov_5.369389, whole genome shotgun sequence.
ACCESSION   DAOPWU010000303 DAOPWU010000000
VERSION     DAOPWU010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21021_length_4243_cov_5.369389"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PKM23_12630"
     CDS             complement(<1..1041)
                     /locus_tag="PKM23_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNY92361.1"
                     /translation="MQDNHQEELNDLMKVRRAKLARLQEMQVQPFAYSFVRDHSAREV
                     LDRFEELEEKSVAVAGRIMALRSMGKAAFAHIADSSGRIQIYLRRDQVGDQAYEVFDL
                     TDIGDIVGVTGTVHKTRTGEITIFAQKFELLTKSLRPMPVVKERVEEGQTVTYDAFAD
                     TELRYRQRYLDLMVNREVRQAFVTRSRIITAVRRYLDGRGYLEVETPVLQPLYGGAAA
                     RPFVTHHNALDMTLYLRIADELYLKRLIVGGFDGVYEISKDFRNEGIDRSHNPEFTMM
                     ELYVAYEDYTFMLNLVEEMVGAIAREVCGDAKISYQGQELDFTAPWPRVRMFEALQRY
                     TGMDLYGKSRDEL"
     gene            complement(1158..2270)
                     /gene="alr"
                     /locus_tag="PKM23_12635"
     CDS             complement(1158..2270)
                     /gene="alr"
                     /locus_tag="PKM23_12635"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNY92362.1"
                     /translation="MRPTWAEIDLGAIAFNLQGIRRQVAPAGIMAVVKADAYGHGALQ
                     VSRVALQEGASHLAVAVIDEAIALRKAGIQAPLLVFGGFSHEDARLFIDWELDATIFD
                     RQGLALLSQAARKRNRPVRVHVKVDTGMGRLGVDWREAASFVAEVQGAEGVELTGLYT
                     HFATSDALDKSYANLQLLRYKSLIDELEGKGVRIPLKHTANSGAILDMPGSWFDLVRP
                     GIMMYGHYPSAETTKSVGIHPAMSFKTTVIQAKRVERGQSVSYGRTFIADRPMTIATL
                     PVGYADGYNRLLSNRGEVLIRGRRFPVAGRVCMDLIMAAAGAEEQIRAGDEVVLFGRQ
                     GDEEISVASICTLLGTIPYEVTCWVSKRVPRVYMHG"
     gene            complement(2346..3456)
                     /gene="prfB"
                     /locus_tag="PKM23_12640"
     CDS             complement(join(2346..3395,3397..3456))
                     /gene="prfB"
                     /locus_tag="PKM23_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705954.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNY92363.1"
                     /translation="MNLDIKSTLKDHVERIAALRGPFDIETKENEKAILEARITQPGF
                     WDDNETAQQVMRDIAERRNWIDAWQKLAQRAADLRDLYELAESESDAGVLKDLETDCI
                     ELEKSVADLEFRKMLGGPGDDKSAILTIHPGAGGTESQDWAQMLMRMYLRWIEREGYE
                     SDILDLQDGDEAGIKSVSIEVKGSYAYGHLKAEAGVHRLVRISPFDANKRRHTSFASV
                     FVYPEVDSSIEVEVDEKDLRIDTYRSSGAGGQHVNKTSSAIRITHIPTGIVVSCQNER
                     SQHRNKDSAMKILMSRLYQLKLEEQQAKLAKLESTKRDIAWGSQIRSYVFHPYNMVKD
                     HRTDHETSNIQAVMDGDLNEFIRAYLMEFGLAGGK"
     gene            3773..>4243
                     /locus_tag="PKM23_12645"
     CDS             3773..>4243
                     /locus_tag="PKM23_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92364.1"
                     /translation="MISSAGTAQEYTAGLLAGGTWQGPGLELQATASRFAQGFPFILR
                     LAAGRYSLDPGDPAAARRIFINDNTNGVPSKDGHFWSLRLDFLYPVQWFSWKRLYFYG
                     GPRRSYFTGHFEYVGGNEDFEVTSGSWGVGAGLEKHYAINRNLDLLLTAGIDYHA"
BASE COUNT          777 a         1254 c         1316 g          896 t
ORIGIN      
        1 gagctcgtcg cggctcttgc cgtaaagatc catccctgta tagcgctgca gggcttcgaa
       61 catgcgcacg cgcggccagg gggcggtgaa atcgagctcc tggccctggt aggaaatttt
      121 ggcatccccg cagacttccc gggcgatggc tccgaccatc tcttcgacca ggttcaacat
      181 gaaggtgtaa tcctcatagg cgacgtagag ctccatcatg gtgaactcgg ggttgtggga
      241 gcggtcgatg ccctcgttgc ggaaatcctt gctgatctca tagaccccgt cgaagccgcc
      301 gacgatgagg cgcttgagat agagctcatc ggcgatgcgc agatagaggg tcatgtcgag
      361 ggcgttgtga tgggtcacga agggccgcgc cgccgcgccg ccgtagaggg gctgcagcac
      421 cggggtttcc acctcgagat agcccctgcc gtcgagatag cggcgcacgg cggtgataat
      481 gcgcgagcgg gtgacaaaag cctggcgcac ctcgcggttg accatgaggt cgaggtagcg
      541 ctggcggtag cgcagctcgg tgtcggcgaa ggcatcatag gtcaccgtct gcccctcctc
      601 gacgcgctcc ttgaccaccg gcatcgggcg cagactcttg gtgagcagct cgaacttttg
      661 ggcgaagatg gtgatctcgc cggtgcgggt cttgtggacc gtgccggtga caccgacgat
      721 gtcaccgata tcggtcagat caaagacctc gtaggcctgg tcaccgacct ggtcgcggcg
      781 gaggtagatc tggatgcgcc ccgagctgtc agcgatgtgg gcaaaggcag ccttgcccat
      841 gctgcgcaaa gccatgatcc gcccggcgac agcgacgctt ttctcctcga gttcctcgaa
      901 gcggtcgagg acctcgcggg cggagtggtc gcgaacaaaa gagtaggcga agggctggac
      961 ctgcatctcc tgcagccggg cgagcttggc ccggcgtacc ttcatcaaat cattcagttc
     1021 ttcctgatgg ttgtcttgca tagcacctca ttgattgtgc agaaatttaa cctgaaaata
     1081 tctcatacag cgtcacggag aggagcgcga atccgcaaag ccggtcagtc ctggtggatt
     1141 ctgcggcagc gatgacatca gccgtgcatg tagacccgcg ggacgcgttt ggagacccag
     1201 caggtcacct cataggggat ggtgccgagc agtgtgcaaa tcgaggcgac gctgatctct
     1261 tcatcaccct ggcggccgaa aaggacgacc tcatcgcccg ccctgatctg ctcctcggcg
     1321 cctgcggcgg ccatgatcag atccatgcag acccgcccgg ccaccggaaa gcgccggccc
     1381 cggatcagca cctcaccgcg gttgctgagc agacggttgt aaccgtcggc gtagccgacc
     1441 ggcagggtgg cgatggtcat cggccggtcg gcgatgaagg tgcggccgta gctgacgctc
     1501 tgtccgcgct cgacccgttt ggcctggatg acggtggtct tgaaactcat cgccggatga
     1561 atcccgacgc tcttggtggt ttctgctgag ggatagtggc cgtacatcat gatcccggga
     1621 cgcactagat cgaaccagct ccccggcatg tccagaatcg cgccgctgtt ggctgtatgc
     1681 ttgagaggaa tgcgtacccc cttgccctcg agctcgtcga tcaggctttt ataacgcagg
     1741 agctggaggt tggcatagct tttgtcaagg gcatcggagg tggcgaaatg ggtgtagagg
     1801 ccggtgagtt ccaccccctc ggcgccctgc acctctgcga caaaggaagc ggcttcgcgc
     1861 cagtccactc cgagccgacc catgccggta tcgaccttga cgtggacgcg caccggccgg
     1921 ttgcgcttgc gggcggcctg gctgagcagg gccagcccct gccggtcgaa aatggtcgca
     1981 tcgagctccc agtcgatgaa gagccgggca tcctcatggc tgaatccccc gaagaccagc
     2041 aggggggcct gaatgccggc cttgcgcagg gcgatcgctt catcgatgac ggcgacggcc
     2101 aggtggctcg ctccctcttg gagggcgacc cggctgacct gcagggcgcc atggccatag
     2161 gcatccgcct tgaccaccgc catgatgccc gccggggcca cctgcctgcg gatgccctgc
     2221 agattgaaag ctatcgcccc gagatcgatc tccgcccatg tcggccgcat tagatctcct
     2281 ccgcgaaggg cccgttcata accgtctcat gatcgccggt aaggagtgct attctcgaag
     2341 acatcctatt tgccccccgc caggccgaat tccatcaaat aggcgcggat gaactcgttg
     2401 aggtcgccat ccataaccgc ctggatgttg ctggtctcat ggtcggtgcg atggtccttg
     2461 accatattgt agggatgaaa aacataggag cggatctggc tgccccaggc gatatcgcgc
     2521 ttggtgctct cgagcttggc cagtttggcc tgctgctcct cgagcttgag ctggtagagc
     2581 cgggacatga ggatcttcat cgccgaatcc ttgttgcggt gctgggagcg ctcgttctgg
     2641 caggagacca cgatgcccgt cgggatgtgg gtgatgcgga tggccgagct ggtcttgttg
     2701 acgtgctggc ctccagcccc gctcgagcgg taggtgtcga tgcgcaggtc cttctcatcc
     2761 acctccactt ctattgagct gtcgacctcg ggatagacaa agaccgaggc gaaggaggtg
     2821 tggcggcgct tgttggcgtc aaagggggag atgcgcacca ggcggtggac tccggcctcg
     2881 gccttgaggt ggccgtaggc gtaggacccc ttgacctcaa tggagacgct cttgatgccg
     2941 gcttcatcgc cgtcctggag gtcgaggata tccgattcgt agccctcgcg ctcgatccag
     3001 cgcagataca tgcgcatcag catctgggcc cagtcctggg attcagtgcc gccggccccg
     3061 gggtggatgg tgaggatggc gctcttgtcg tcgccgggtc cgcccagcat cttgcggaac
     3121 tcgaggtcgg caacgctctt ttccagctct atgcagtcgg tctcgagatc cttgagcacg
     3181 cccgcatcgg attcactctc ggcgagctca taaagatcgc gcaggtcggc cgctctttgc
     3241 gccagtttct gccaggcatc gatccagttg cgccgctcgg cgatgtcgcg catcacctgc
     3301 tgggcggttt cgttatcatc ccagaagccg ggctgggtta ttctggcctc gagtatggcc
     3361 ttttcgtttt ccttggtttc gatgtcaaag ggacctccgc agggcggcga tgcgttccac
     3421 gtggtccttg agcgtgctct ttatgtccag attcataggg ccttctgctt gcagtttgca
     3481 tttttaaagt aataattaat gtacgacata cccggcaaag aatcaagatt ttaaaagcgc
     3541 cacagggtat gccccgaaat ctggctcccc tgcgacacct gcgccgcgca accacccacg
     3601 actgcagctc ccgcgctgtt tcgccgccgg atccgcattc aatcctcaat tttgtgacga
     3661 accggattgc agattcacgg cagatgatct atcttaaggt tgtaatttaa cagctcatat
     3721 tacggagtac gggatgatgg gacgaataaa attaatcctg gcagcaatta tcttgatctc
     3781 ttctgctggt acagcgcagg agtatacggc cggtctcctt gccggcggca cctggcaggg
     3841 gcctgggctg gagctgcagg caacggcatc ccggttcgcc cagggattcc catttatcct
     3901 gcgcctggcc gccggacgct acagcctcga tccgggtgat cctgccgctg cacgcaggat
     3961 attcatcaat gacaacacca atggcgtgcc cagcaaggat ggccatttct ggagcctgcg
     4021 gctcgatttc ctctatccgg tgcagtggtt cagttggaaa cgtttgtatt tctacggcgg
     4081 gccgcggcgt tcctacttta cggggcattt tgaatacgtc ggcggcaacg aggattttga
     4141 ggtcacctcc ggcagctggg gggttggtgc gggacttgaa aagcattatg ccatcaaccg
     4201 caatctcgac ctgcttttga cggccgggat tgactaccac gcc
//
LOCUS       DAOPWU010000304         4238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21055_length_4238_cov_3.488645, whole genome shotgun sequence.
ACCESSION   DAOPWU010000304 DAOPWU010000000
VERSION     DAOPWU010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21055_length_4238_cov_3.488645"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2710
                     /locus_tag="PKM23_12650"
     CDS             <1..>2710
                     /locus_tag="PKM23_12650"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNY92365.1"
                     /translation="QGIYSLVTGIYGPPPNRSGDGYVYILIHDIKDNYDPDSGKRVYI
                     AGYFSPTDQTNGTYSNRKDLINVDCYPQNPSHAGALATVAHEFQHLIHNGQDADEDEG
                     GLWVNEGASEYSEVLCGYSLRMPTYYLLHPERSLTQFDASDENLWDYQKVALWTYYLA
                     EKFGPELIGAIVKDPKNSLEGVRSALIKRGITLSFEDIFSNFAVANYADNPALGENSY
                     YSYNKIRLPSLPASTPHSVYPLKDQNRTLPNYSMGYYRFTGEDSTAVLHLQGKPGAEF
                     RARIYAPGATPSVQSVALDATNTGQFPLNNIGVSAEADVLTAISLGSSNAFTYSVTSD
                     LTDRAMPTIFTGPQELIPTGTSTTITWTTDERSTSLLEYGLSSAYGSSLADTALVTSH
                     LVTLTGLQPNTTYHYRVGSIDAYGNGPRYSADFQFRTASGSDNTIAVVEQSHSYAYSG
                     RSMARTQDGTLHLLYHQVSGSRRFVYHKSTADNGATWSDPRLVDESLYHSGMPAVAAD
                     HLGRLHVAWHARAGSAEDKLKIYYSRSDDNGLSWSAPVCIGSHDQNDLLYAAIAADAE
                     SNPHVVWNIALYDDDYAGDIYYAGSGDGGRTWNAQTMISSGAGDRRCHVPVIDITPAG
                     EIWVAWTDGLFDQESRKIWHTRSTDHVTWSSPAAVTASGVLYDRYPSLVIDDRSVVHL
                     AYTDNYTPGDIRIMYIRYDGGSWSEPIPVAQSTTGNVSAPSLTCDSLGVLYLVYRDDQ
                     GSATLGRYASAPAETADGPALAKPSASDSDIFFALCSKGGWLSGGNISSDDVANEYPE
                     TPRRVQPGALDAIWMRTVTTTDNRIQFLHLQTEPETTRQPLGISVISPEADAGGVSYF
                     KQAFFVQVDFNQRVISDSITAATFRVTSAAQGPLVGFITYDQSQRRL"
     assembly_gap    2711..2720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2721..3801
                     /locus_tag="PKM23_12655"
     CDS             <2721..3801
                     /locus_tag="PKM23_12655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92366.1"
                     /translation="LFQVNVNLLPEDLITVWLSGSIPQEGGVGLDGNGNGIAEGSPAD
                     DFSWSFRTGAPDLQAPAFTIGIAQNPVLNRYMDIYLFPSEVLLRNPAVEIGGSIFPAL
                     RVPGEAFIYKADYRLDQSGVMQIKATGEDLAGNVGQGERLFTAQFMLAESGGMLSSPD
                     GRFSLEVGERSLPHDLYLTLVPEKGVGAEADPEWVIGPERLSLARPVQLRVAAPAQEE
                     KRYFIEQKRPDGSWKPLPGAVEEGVVKASAQSLGTFRLASVTQVIPRQFGLHQNYPNP
                     FRRGVETTTFHLDLPLQREVEVAVYNLLGERIRLLTRGTLAAGVHTLHWDGRDENRNA
                     VASGVYFYRCVFPGGSFTRKMLILQ"
     gene            3848..>4238
                     /locus_tag="PKM23_12660"
     CDS             3848..>4238
                     /locus_tag="PKM23_12660"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNY92367.1"
                     /translation="MNTSSPVTGSIATGGRILLAALVGALLWSAGCGGDNGTNPVNYP
                     KPRIVDIPQVSELSASQATISWTTDVDATSAVLFGTQSGSYTRKDSSNALKSAAHKRT
                     LTSLQSNTLYYYIVRSRSEGGAVASEEG"
BASE COUNT          810 a         1486 c         1176 g          756 t
ORIGIN      
        1 tcagggcatc tacagcctgg tcaccgggat ctatggcccg ccgccgaacc gctccggcga
       61 tggctatgtc tatattctca ttcacgatat caaggataac tacgacccgg acagcggcaa
      121 acgggtctat atcgccggct atttcagccc caccgatcag accaacggca cctacagcaa
      181 ccgcaaggat ctcattaacg tcgactgcta cccccagaat ccctcccatg ccggcgccct
      241 ggcgaccgtc gctcatgagt ttcagcatct catccataac ggccaggatg ccgatgagga
      301 cgaaggcggc ctctgggtga acgagggcgc ttccgagtac agcgaggtgc tgtgcggata
      361 cagcctgcgc atgccgacct actatcttct ccatcccgag cgctcgctca cccagttcga
      421 tgccagcgac gaaaatctct gggactatca gaaagtcgcc ctctggacct attatctggc
      481 ggagaaattc ggcccggagc tgatcggcgc cattgtcaag gatcccaaga acagtctgga
      541 gggggtgcgc agcgccctga tcaagcgcgg catcaccctc agttttgagg atatttttag
      601 caatttcgcc gtcgccaact atgcggataa tcccgctttg ggcgagaaca gctactactc
      661 ttataacaaa atccgcctgc ccagcctgcc agcctcgact ccgcactccg tctatcccct
      721 gaaagaccag aatcgaaccc tccccaacta ttcgatgggc tattaccgct tcaccggcga
      781 ggactcgacc gctgtgcttc atctgcaggg caagcccggc gcggagttcc gggcccggat
      841 ctatgcaccg ggcgccaccc cctccgtgca aagcgtcgcc ctggatgcga ccaatacagg
      901 gcagtttccc ctcaacaata tcggcgtctc cgctgaggcc gatgtgctca ccgccatcag
      961 cctcggctcc agcaatgcct ttacctactc ggtgacaagc gacttgaccg accgggccat
     1021 gccaacgatc ttcaccggac cccaggagct gatccccacc ggaacctcga caaccatcac
     1081 ctggaccacc gatgaacgct ccaccagtct gttagagtac ggactgagca gcgcttatgg
     1141 cagctccctg gccgataccg ccctggtcac ctcgcacctc gtcaccctga ccgggctcca
     1201 acccaacacc acctaccact accgcgtcgg ctccatcgac gcctatggca acggaccccg
     1261 ttacagcgcc gactttcagt ttcgcacggc ctccggcagc gataatacca tcgcggtcgt
     1321 cgagcagagc cactcctatg cctattcagg ccggagcatg gcgcgcaccc aggacggcac
     1381 gctccatctg ctctaccatc aggtctcggg aagcaggcgc tttgtttacc acaagagcac
     1441 cgccgataac ggcgccacct ggtccgatcc ccgcctcgtc gatgagagcc tctaccacag
     1501 cggcatgccc gccgtcgctg ccgatcacct cggccgcctc cacgtcgcct ggcacgcccg
     1561 cgccggttcg gccgaggaca agctgaaaat ctactacagc cgttcggacg ataatggcct
     1621 gagctggtcg gcgcccgtct gcatcggctc ccacgaccag aacgacctcc tctatgccgc
     1681 gatagcggcc gatgcggaga gcaatcccca tgtggtctgg aatatcgccc tttatgacga
     1741 cgactatgcc ggcgacatct attacgccgg ctcgggggac ggcggacgga cctggaacgc
     1801 ccagacgatg atcagcagcg gcgcgggcga ccggcgctgc cacgtccccg tcatcgatat
     1861 cacccccgcg ggcgaaatct gggtggcctg gaccgacggc ctttttgacc aggagagccg
     1921 caagatctgg cacacccgca gtacggacca tgtcacctgg agcagcccgg ccgctgtcac
     1981 cgcctcgggc gtgctctatg accgctatcc ctccctggtg atcgacgacc gctcggtcgt
     2041 ccacctcgcc tacaccgaca actacacacc gggcgatatc cgcatcatgt atatccgcta
     2101 cgacggcggg agctggtctg agcccatccc ggtggcccag tcgaccaccg gcaatgtcag
     2161 cgcccccagt ctgacctgcg attccctggg ggtcctctat ctcgtctatc gcgacgatca
     2221 gggatcggcg accctcggcc gctatgcctc cgctcccgcc gagacagccg atggccccgc
     2281 gctcgccaaa ccctccgcct cggacagcga catctttttc gccctgtgta gcaagggtgg
     2341 atggctctcg ggcggcaaca tcagcagtga tgatgtggcc aacgagtatc cggagacgcc
     2401 gcggcgggtc cagcctgggg cgctggatgc catctggatg cgcacggtca cgaccaccga
     2461 caaccgcatc cagttcctcc acctgcagac cgaacccgag actacccgcc agcccctggg
     2521 gatcagcgtc atctctcccg aggcggacgc cgggggagtc tcctacttca agcaggcctt
     2581 tttcgtccag gttgatttca atcaacgggt gatcagcgac tcgatcacgg cggccacctt
     2641 cagggtgacc agcgctgccc agggccccct cgtcggtttt atcacctacg atcagagcca
     2701 gcggcgtctc nnnnnnnnnn cctctttcag gtcaatgtca atctgctccc cgaggacctc
     2761 atcacggtct ggctgagcgg cagcatcccc caggaggggg gcgtcgggct ggacggcaac
     2821 ggcaacggca tcgctgaggg gtcgcccgcc gatgatttca gctggagctt ccggaccggg
     2881 gccccggatc tgcaggcgcc ggccttcacc atcggcatcg cccagaatcc cgtgctcaac
     2941 cgctacatgg acatctatct tttcccatct gaggtgctgc tcaggaatcc cgccgtcgag
     3001 atcggcggca gcatcttccc cgccctgcgc gtacccggcg aggcctttat ctacaaggcc
     3061 gattaccggc ttgatcagag cggggtcatg caaatcaagg ccaccggtga ggatctggcc
     3121 ggaaatgtgg gccagggcga gcggcttttc accgcccagt tcatgctcgc cgaaagcggg
     3181 ggcatgctct cctcacctga tggccgcttt tcgcttgagg ttggggagag gagcctcccc
     3241 cacgacctct atctgacgct ggtgcccgaa aagggcgtgg gggcggaggc cgatcccgag
     3301 tgggttattg gcccggagcg gctgagcctg gcgcgccccg tgcagctgcg cgtcgccgcg
     3361 ccagcgcagg aggagaagcg ttatttcatc gagcagaagc ggccggatgg atcatggaag
     3421 cctttgcccg gcgccgttga ggagggcgtg gtcaaagcct cggcacagag cctgggtacc
     3481 ttccgcctgg cctccgtcac tcaggtcatc ccccggcagt tcggcctcca tcagaactat
     3541 cccaacccct tccgccgcgg cgtggagacg accactttcc atctcgatct gcccctgcag
     3601 cgggaggtcg aggtcgccgt ctacaacctc ctcggagaac gcatccgcct cctgacccgg
     3661 ggtactctcg cggcgggtgt gcataccctg cactgggacg gccgcgatga aaaccggaac
     3721 gccgtggcca gcggggtcta tttctaccgc tgtgtttttc ccggcggctc cttcacccga
     3781 aaaatgctca tcctccagta gaagggagcg atgaatgcaa cccgcatcca gaaaagacgg
     3841 aaagcgcatg aataccagtt cccctgttac cggatccata gccaccggcg gccgcatcct
     3901 gctcgccgcc ctcgtcggcg ccctgctctg gagcgccggc tgcggcggcg acaacggcac
     3961 caatccggtc aactatccca agccgaggat cgtggatatc ccacaagtca gcgagctctc
     4021 ggccagccag gcgacgatca gctggaccac cgatgtcgat gccacctcgg cggtgctctt
     4081 tggcacccag agcggctcct acacccgcaa ggacagcagc aatgccctca aatccgccgc
     4141 ccacaaacgc actctcacct cgctgcagtc caataccctc tactactaca tcgtccgctc
     4201 gagatcggag ggaggcgcgg tcgcttccga ggagggca
//
LOCUS       DAOPWU010000305         4235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21072_length_4235_cov_4.983971, whole genome shotgun sequence.
ACCESSION   DAOPWU010000305 DAOPWU010000000
VERSION     DAOPWU010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21072_length_4235_cov_4.983971"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..595
                     /locus_tag="PKM23_12665"
     CDS             134..595
                     /locus_tag="PKM23_12665"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNY92368.1"
                     /translation="MYGIDGAVLAWFFIGFFLILTELVMPGFIVIFFGVGAWATCLLL
                     ELGLIHSFNAQLVVFLIASGASLLLFRKQGKTLFDGKKSGKLGPFESLDDYVGKRAVV
                     VEAIKPGDPNGRVEFNGMLWSAESAETIPAGTPVEIIKRENLTLVVRSIAS"
     gene            686..1636
                     /locus_tag="PKM23_12670"
     CDS             686..1636
                     /locus_tag="PKM23_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HNY92369.1"
                     /translation="MLQAETLILIGLIILAIIILSKSAKVVPQKTVYIVERLGKYSGT
                     LEAGFHILIPFIDRIRYIHSLKENAIDVPEQSCITKDNIQVDIDGILYFVIVDPVKAS
                     YGINDYAFASIQLAQTTMRSEVGKIDLDKTFEEREKINSEIVAAVDKASMPWGLKVTR
                     YEIKNIVPPPSIKDAMEKQMRAEREKRALIAESEGDKQAKINRAEGDKQEAIAKSEGE
                     KMKRINEAEGRAQEIERVAEATAKGIREIAAAIAAPGGSQAVNLRIAEQYLGEFGKLA
                     TKSNTMIIPANLTDVAGTVATVARIFSETNKGMNEPPKPA"
     gene            1730..3343
                     /locus_tag="PKM23_12675"
     CDS             1730..3343
                     /locus_tag="PKM23_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNY92370.1"
                     /translation="MRVSKKKNGLTLQAYAGTTGVLLAMNASAAKRKGLLGFAIEREG
                     PAPGRVRWLQGSLRFPGESQDVNAAVDSNLGPIQKFRWSDYSVYANTPYRYKIYGVYG
                     RPGKLRYLDGPEVELRSESLLEGRHQIIFNRAAAASQAYARRFGNANPDLPDQAEARA
                     WLSRGLYERLQSFFDQALDASWALDVAVYEIELPETVAALARAMERGVHVRIIYHGRS
                     EDPQTALNEQTLEPLPGHIKTRRITSAIFHHKFCVLSRLQGDGSRAPAALLTGSTNFT
                     ANAVYRQANVLHIMEDPDIAARYLELFDRLFNGMAPGDTRKYINNTNPAMAGAAPQVL
                     FSPRSHLTDLQEVAGIIRRARRDLVFCTAFNLLDEIEEALLGGEDDDVIRYGLQNTKS
                     RITGTHRHARFVTPAFLASGLEGFLKESYAGQEGNILIHLKTIVTDFSTASPTVITGS
                     NNFSRSASASNDENLLIISGETAAADIYVTEMMRLYDHYRFRYNIKTRSGGGTPGRLV
                     LAADDHWTDRYYAPQSLEYYERVRFCAPE"
     gene            complement(3356..4057)
                     /gene="radC"
                     /locus_tag="PKM23_12680"
     CDS             complement(3356..4057)
                     /gene="radC"
                     /locus_tag="PKM23_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNY92371.1"
                     /translation="MAEYPNRITDWPEEERPRERLLRHGPDALSEAELLAIILRVGAG
                     KTTAVDLARRLISEFDGLRGLDAAPVASIRQIKGMGDAKACQVKAALELAKRFSQQKW
                     NRQEVRVQNSGEVYHYLHLRLRDLSREVFQVLFLTTRHDIVADKILFEGSLTESVVSP
                     REIITEALHHAAAALILVHNHPSGDPHPSIEDKKVTDKIIQACRPFDLAVLDHLIIGR
                     DSYYSFADQGLIAAG"
BASE COUNT          810 a         1463 c         1256 g          706 t
ORIGIN      
        1 cccgccggcc gcatccgcgt tttacggctg ataaaaggct tgcttttatt ccgcgccggc
       61 gctaacttac aattatccat ccatcggcgg caccagccgc cccgagcgag aaccgatccg
      121 gaaggagagt gtaatgtacg gaatcgacgg cgccgtcctg gcgtggtttt tcatcggttt
      181 ttttctcatc ctcaccgaac tggtgatgcc gggctttatc gtcatttttt tcggcgtcgg
      241 cgcctgggcg acctgcctcc tgctcgagct cgggcttatc cactccttca acgcacagct
      301 agtcgtcttt ctgatcgcct ccggggcctc cctcctcctc ttccgcaagc agggcaaaac
      361 cctcttcgac ggcaagaaat cgggcaagct cggccccttt gaatccctgg acgattacgt
      421 cggcaagcgc gctgtagtgg tcgaggcgat caagccgggc gatcccaacg gcagggtgga
      481 gttcaacggc atgctgtgga gcgccgaatc cgcggaaacg atccccgccg gcacacccgt
      541 cgagatcatc aaacgtgaaa atctgaccct ggtggtaaga tccatagcct cataaccccg
      601 cccggcgccc ggatggaagc ggcgctatga gccccgcagg cgcccgtgcc gctggccgtt
      661 caatcctctt cgaaaggagc attccatgct gcaagccgaa accctgatcc tcatcggact
      721 catcatcctg gcaatcatca tcctctcgaa atcggccaag gtggtgccgc aaaagaccgt
      781 ctacatcgtc gagcgcctcg gcaagtacag cggcaccctc gaggccggct ttcatatcct
      841 tatccctttc atcgaccgca tccgctacat ccattccctc aaggagaacg ccatcgacgt
      901 cccggagcag tcctgtatca ccaaggataa cattcaggtc gacatcgacg gcattctcta
      961 ttttgtcatc gtcgacccgg tcaaggcttc gtacggcatc aacgactatg ccttcgcctc
     1021 gatccagctc gcgcagacca ccatgcgttc cgaggtcggc aaaatcgatc tcgacaagac
     1081 ctttgaggag cgggaaaaga tcaattcgga gatcgttgcc gcggtcgaca aggcctcgat
     1141 gccctggggg ctgaaggtga cccgctacga gatcaagaac atcgtcccgc cgccgagcat
     1201 caaggacgcg atggagaagc agatgcgcgc cgagcgcgaa aagcgcgccc tcatcgccga
     1261 gtcggagggc gacaagcagg ccaagatcaa ccgcgccgag ggcgacaagc aggaggcgat
     1321 cgccaaatcg gaaggcgaaa agatgaagcg catcaacgag gccgaaggcc gcgcccagga
     1381 gatcgagcgc gtcgccgagg ccaccgccaa ggggatccgc gagatcgccg cggccattgc
     1441 cgccccgggc ggcagccagg cggtcaacct gcgcatcgcc gagcagtacc tgggcgaatt
     1501 cggcaagctg gcgaccaaga gcaacaccat gatcatcccc gccaacctga ccgatgtggc
     1561 gggcacggtg gcgacggtgg cgcggatctt cagcgaaacc aacaagggga tgaacgagcc
     1621 gcccaagccg gcctaggcga tcctcgtcag gggcgccggc tcagccccgg gcccggagct
     1681 gccgggagac taacaagtca agggcgggcg ccaaggaagg gaggcggatg tgcgcgtcag
     1741 caaaaagaaa aacggattga ccctgcaggc ctacgccggc accaccgggg tgctgctggc
     1801 gatgaatgcc tcggcggcca agcgcaaggg actgctcgga ttcgccatcg agcgggaggg
     1861 cccggctccc ggccgggtgc gctggcttca gggcagcctg cgttttcccg gcgaaagcca
     1921 ggacgtcaac gccgcggtcg acagcaacct cggccccatc cagaaattcc gctggtccga
     1981 ctattcggtc tacgccaaca ccccctaccg ctataaaata tacggcgtct acggccggcc
     2041 gggcaagctg cgctacctcg acggccccga ggtcgagctg cgcagcgagt cgctgctgga
     2101 ggggcggcac cagatcatct tcaaccgtgc ggcggcggcc agccaggcct acgcccgccg
     2161 ctttggcaat gccaatcctg atcttccgga tcaggccgag gcgcgcgcgt ggctctcgcg
     2221 cggcctctat gagcggctgc agtccttttt cgaccaggcc ctcgacgcca gctgggccct
     2281 cgacgtcgcc gtctacgaga tcgagctgcc cgagaccgtc gccgccctgg cgcgggccat
     2341 ggagcgggga gtgcacgtgc gcatcatcta tcacggccgc agcgaggatc cccagaccgc
     2401 cctcaacgag cagactctcg aacccctgcc cggccacatc aagacccgcc gcatcacctc
     2461 cgccatcttt catcacaaat tctgcgtgct cagccgcctc cagggcgacg gcagccgcgc
     2521 tccggccgcc ctgctcaccg gctcgaccaa tttcaccgcc aacgccgtct accgccaggc
     2581 caacgtcctc cacatcatgg aggaccccga tatcgccgcc cgctacctcg agcttttcga
     2641 ccggctcttc aacggcatgg cccccggcga cacccgcaaa tacatcaaca acaccaatcc
     2701 cgccatggcg ggggcagccc cgcaggtgct cttcagcccg cgctcgcatc tcaccgacct
     2761 ccaggaggtg gccggaatca tccggagggc caggcgcgac ctcgtcttct gcaccgcctt
     2821 caacctcctc gacgagatcg aggaggccct tctcggcgga gaggatgatg acgtcatccg
     2881 ctacggcctg cagaacacca aatcgcgcat caccggcacc caccgccatg cccgcttcgt
     2941 cacccccgcc tttctcgcca gcggactgga gggctttctc aaggaatcct acgccggcca
     3001 ggagggcaac atcctcatcc acctcaagac catcgtcacc gatttcagca ccgccagccc
     3061 caccgtcatc accggctcga acaacttttc gcgcagcgct tcggccagca acgacgagaa
     3121 cctgctcatc atcagcggcg agaccgcggc ggccgacatc tatgtgaccg agatgatgcg
     3181 cctctacgat cactaccgct tccgctacaa catcaagacc cgcagcggcg gcggcacgcc
     3241 cggccggctg gtgctggccg cggacgatca ctggaccgac cgctattacg ccccgcagag
     3301 cctggagtac tatgagcggg tgcgcttctg cgcgccggag tagaccggcg ccgccctatc
     3361 ccgccgcgat cagcccctgg tccgcaaagc tgtagtacga atcccggccg atgatgagat
     3421 gatcgagcac ggcgaggtcg aagggccggc aggcctggat gatcttgtcg gtcaccttct
     3481 tgtcctcgat ggagggatgg ggatccccgc tggggtggtt gtggacgagg ataagggccg
     3541 cggcggcatg gtgcagggcc tcggtgatga tctcccgcgg cgaaaccaca ctctcggtca
     3601 gagagccctc gaagaggatc ttgtcggcga cgatgtcgtg gcgggtggtg aggaagagga
     3661 cctggaagac ctcgcgcgag aggtcgcgca gacgcagatg cagatagtga tagacctccc
     3721 cggagttctg cacccgcacc tcctgacggt tccacttttg ctggctgaag cgcttggcca
     3781 gctcgagggc ggccttgacc tggcaggcct tggcgtcgcc catgcccttg atctggcgga
     3841 tggaggcgac cggggcggcg tcgaggccgc gcaggccgtc gaactcgctg atgagccgac
     3901 gcgccagatc gacggcggtg gttttgcctg cgccgacgcg gaggatgatg gcgagcagct
     3961 ccgcttcgga gagggcgtcg ggcccgtggc gcagcagccg ttcgcgcggc cgctcctcct
     4021 cgggccagtc ggtgatgcgg ttgggatatt cagccatggc tttctccctc atgttcgctg
     4081 cagccctggt ttccgcctcc tcccaccggc ttctcgctcc caccagattc tcgcgccaac
     4141 cggctccctc tcgctcccac ccaggctctc acacgccccc gccggctttt cccctacccg
     4201 atgatagcga ttttgccaag gcggcgcaag caaaa
//
LOCUS       DAOPWU010000306         4226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21133_length_4226_cov_3.302086, whole genome shotgun sequence.
ACCESSION   DAOPWU010000306 DAOPWU010000000
VERSION     DAOPWU010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21133_length_4226_cov_3.302086"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKM23_12685"
     CDS             complement(<1..240)
                     /locus_tag="PKM23_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92372.1"
                     /translation="MDGKPGRQQLCISCGSYLHCCRNCRFYDPLAYHACREPQAEWVK
                     EKESGNFCDYFSPADQPTGAGSKAEEARRKLDALFG"
     gene            complement(361..984)
                     /locus_tag="PKM23_12690"
     CDS             complement(361..984)
                     /locus_tag="PKM23_12690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNY92373.1"
                     /translation="MNKDFYQTIAGAAESALKIKGSRFLGFAAPAAGREEAEATLAAL
                     RKRFFDATHHCYAWRLGSGTGENSRFSDDGEPSGTAGKPILQVINGRDLTNLILVVVR
                     YFGGTKLGTGGLVRAYSDAAVLTLDQTVIRQVWWTRSLEFQLGYDHLSTVMKLIDRHE
                     GVIEGTEYGVEVRMKVAFRLREAEPFSAAARDMTHGQIVFYSVEGKS"
     gene            complement(1017..2174)
                     /locus_tag="PKM23_12695"
     CDS             complement(1017..2174)
                     /locus_tag="PKM23_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNY92374.1"
                     /translation="MDYYLTEMQATLQQLAREFAAGTIAPAAAEYDRSAEFPWPIVRE
                     MARMDFFRLWIPEEYEGMGGGITEMALVTEEFSRACGGIALALAGTALGTFPLLIAGT
                     DEQKRRHLPVLAAGEGLAAFCLTEPEAGSDAGAIQTTARLEGDRYILNGTKHFITNGG
                     VSRYYTVIATTDPSRGARGATAFLVEEGTPGLIFGKKEEKMGIRASATCEVVFQDCAI
                     PRQNLLGKEGMGFIIAMKTLDRSRPGVAAQAVGIAQGALDHAVRYARTRQQFGQPISA
                     FQGVQFMLADMATQVEAARALVYATARMIDAGAKDYGRESAMCKVLAADTAMRVTTDA
                     VQIFGGYGYMKDYPVEKYMRDAKITQIYEGTNQIQRGVIARHVIKESASGL"
     gene            complement(2174..2941)
                     /gene="rnc"
                     /locus_tag="PKM23_12700"
     CDS             complement(2174..2941)
                     /gene="rnc"
                     /locus_tag="PKM23_12700"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNY92375.1"
                     /translation="MQWLKVPWSKLLKTRREVKTPQLEELMRVINYRFHDQSLLIQAL
                     KHRSYLVHTGEDRLQSNERLELLGDSVLGLVVTENLYTEFPDEEEGVLTNYKSLLVNR
                     VNLSRVGHEFGLGRYLLLNDSEERAGGRSRDSILSDAVEALIGAMYLDGGLEPARKMI
                     HKHIVTGLSALLEETQYKNFKSLLLEFCQRENKTGPVYMVENEEGPDHNKVFTVAVFI
                     NGSKHGTGTGTSKKVAEQHAAEQALALLYQKQEKTKG"
     gene            complement(3032..>4226)
                     /gene="fabF"
                     /locus_tag="PKM23_12705"
     CDS             complement(3032..>4226)
                     /gene="fabF"
                     /locus_tag="PKM23_12705"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNY92376.1"
                     /translation="GHTPAQMFDNLVAGKSGISRFEAFDSSAYDTRIAGEIRDLVVTD
                     YIEPKEARRMDRFTQFGVIAAQKCVEDAGIDFGQCDRDRFGVVLGSGIGGMQVFQKEC
                     QVLFERGPARVSPLLIPMLIPDICPGLVSIRHGLKGVNYTTVSACASAAHAIGLAFTH
                     IRLGEADGILAGGSEAPINPIGIAGFNAMHAMSTRNDEPERASRPFDLHRDGFIMGEG
                     GGVLLLEELEHARRRGARIYAELGGYGFSADANHITAPDPSGDGALRSMQQALRSANL
                     PPVSVDYINAHGTSTPLNDKTETLAIKTLFGDHAWRLSISSSKSMLGHLLGASGAAEA
                     IATIMSIQRQIVHPTANLETPDPLCDLDYTPNRARERRVEVALSNSFGFGGHNVTLLL
                     KRFAG"
BASE COUNT          772 a         1252 c         1389 g          813 t
ORIGIN      
        1 gccaaagagg gcatccaatt tgcggcgggc ctcctccgct ttgctccctg cacctgtcgg
       61 ctggtcggcc gggctgaaat aatcgcaaaa gttgcctgac tccttttcct tgacccactc
      121 cgcctggggc tcacggcagg cgtgataggc aaggggatca taaaaacggc agttacggca
      181 gcagtgcagg tagctgccgc agcttatgca gagctgctgc cggccgggtt tgccatccat
      241 aacattttcg cttttgcaat ggtagcagat catgtacact cctcgttcaa gctgcttcac
      301 accacggcgc gaccggcggc gcagccgccg tcggctggag gcagatcatt cgcggcggct
      361 ttagctcttg ccctcgacag aataaaagac gatctgtccg tgggtcatat ccctcgccgc
      421 cgcgctaaag ggctccgcct cccgcagtct gaaggccacc ttcattcgca cctccacccc
      481 gtactccgtt ccctcaatca ccccctcatg gcggtcgatc agcttcatca cggtgctgag
      541 gtggtcgtac cccagctgga actcgagcga gcgggtccac cagacctgcc ggatgacggt
      601 ctgatcgagg gtcaggacgg cggcatcgct gtaggcgcgg accagtccgc cggtgccgag
      661 tttggtgccg ccgaaatagc gcacaacgac gaggatgaga ttggtgagat cgcgtccgtt
      721 gatgacctgg aggatgggct tgccggctgt gcctgagggt tcgccgtcat cggagaagcg
      781 gctgttctcc ccggtgccgc tgcccaggcg ccaggcatag cagtggtggg tggcatcgaa
      841 aaaacgtttg cgcagagcgg cgagagtggc ctccgcctcc tcacgcccgg ccgcgggcgc
      901 ggcaaagccg agaaagcggg agcccttgat cttgagcgcg gattcggccg cgcccgcgat
      961 agtttgatag aaatccttat tcatgatcgg atggccgggc ggggaaagag cccgccctag
     1021 agcccgctgg cggattcctt gatgacatgg cgcgcaatca cgccgcgctg gatctggttg
     1081 gtgccctcat agatctgggt gattttggcg tcgcgcatgt atttttcgac cggataatcc
     1141 ttcatatagc cgtagcctcc gaagatctgc acggcgtcgg tggtgacgcg catggcggtg
     1201 tcggcagcca gcaccttgca catcgccgat tcgcgcccgt agtctttcgc accggcatcg
     1261 atcatgcgcg cggtggcgta gaccagtgcg cgggcggctt cgacctgcgt ggccatgtcg
     1321 gcaagcatga actggacccc ctgaaaggcg gagatgggct ggccgaactg ctggcgggtg
     1381 cgggcatagc gcacggcgtg atcaagggcg ccctgggcga tgccgaccgc ctgggcggcc
     1441 acgccgggac gggagcggtc gagggtcttc atggcgatga tgaatcccat cccctccttg
     1501 ccgagcagat tctgacgggg gatggcgcag tcctgaaaga ccacctcgca ggtggccgag
     1561 gcgcggatgc ccatcttctc ctctttcttg ccgaagatca atcccggcgt cccctcctcg
     1621 acgaggaagg cggtggcgcc gcgggccccg cgcgagggat cggtggtggc gatgacggtg
     1681 taatagcggg ataccccccc gttggtgata aaatgcttgg tgccgttgag gatgtagcgg
     1741 tcgccctcga gccgggcggt ggtctggatc gcccccgcgt ccgatcctgc ctccggttcg
     1801 gtcagacaga aagcggccag cccctccccg gcagccagca cgggaaggtg cctgcgcttc
     1861 tgttcatcgg taccggcaat caggagggga aaggtgccga gggccgtgcc ggccagggcc
     1921 agggcgatgc cgccgcaggc tcgggagaac tcttcggtga ccagggccat ctctgtgatg
     1981 ccgcctccca tcccttcata ctcctccgga atccagaggc ggaaaaagtc catgcgcgcc
     2041 atctcgcgga cgatgggcca ggggaactcg gcactccggt catattccgc tgccgccggg
     2101 gcgatggtgc cggcagcgaa ttcacgggcc agctgctgca gggtggcctg catctcggtc
     2161 agataatagt ccatcatcct ttggttttct cctgcttctg ataaagaaga gccagggcct
     2221 gctcagcggc gtgctgctcg gccacctttt tggaagtgcc ggtgccggtg ccatgcttgc
     2281 tgccgttgat aaaaaccgca acggtgaaaa ccttgttatg atccggcccc tcctcgtttt
     2341 cgaccatata gaccgggccg gtcttgtttt cgcgctggca aaattccagc agcaggctct
     2401 tgaaattctt gtattgggtc tcctccagca aggccgaaag cccggtcacg atgtgcttgt
     2461 gaatcatctt gcgggctggc tcgaggccgc cgtccagata catcgccccg atcagggcct
     2521 cgaccgcatc agagaggatg gaatcccgcg aacgcccgcc ggcgcgctcc tccgaatcat
     2581 tgagcaggag atagcggccc aggccgaatt catgcccgac acggctgagg ttgacccggt
     2641 tgacgagcag ggacttgtaa ttggtcagca ccccttcctc ctcgtccggg aactcggtgt
     2701 agaggttttc ggtaaccacc aggccgagga ccgagtcccc gagcagctcg aggcgctcat
     2761 tggactgcaa gcggtcttcg ccggtgtgga cgagatagga acgatgcttg agggcctgga
     2821 tcaggaggga ctgatcgtgg aagcggtaat tgatgacgcg catcagctct tcgagctgcg
     2881 gggtcttgac ttcgcggcgt gttttgagaa gcttggacca ggggaccttc aaccactgca
     2941 tgggatctcc cacatttgcc agttctgcag agttgacgcc gggctcatgc ggcgtaaggt
     3001 ttgagtggag caaaaagtat agaccggtct gctatccggc gaatcttttc aagagcagtg
     3061 tgacattgtg accgccaaag ccgaacgagt tggagagggc cacctccacc cggcgctccc
     3121 tggcccggtt gggggtatag tcgagatcgc aaagaggatc cggggtttcg agattggcgg
     3181 tcgggtggac gatttggcgc tgaatggaca tgatggtagc gatggcctcg gccgcgcctg
     3241 aggcgccaag gagatggccc agcatcgact tggaggagct gatcgagagg cgccaggcat
     3301 gatcgccgaa aagagtcttg atggccaggg tctcggtctt gtcgttgagg ggcgtggagg
     3361 tgccgtgggc gttgatgtaa tcgacggaca cggggggaag attggcactg cgcagggcct
     3421 gctgcatcga gcgcaaggcc ccgtcgccgg agggatccgg cgcggtgatg tggttggcat
     3481 cggcgctgaa gccatagccg cccagctctg cataaattct cgccccgcgt ctgcgggcat
     3541 gttcgagttc ctcgagcaaa aggacgcccc ccccctctcc catgataaaa ccgtcgcggt
     3601 ggagatcgaa gggccggctg gcgcgctcag gttcgtcatt gcgggtggac atggcgtgca
     3661 tggcattgaa gccggctata cctatcggat tgatcggggc ttcggagccg ccggcaagga
     3721 tgccgtcggc ttcgccgagg cggatatggg tgaaggccag cccgatggcg tgggcggccg
     3781 aggcgcaggc tgaaaccgtg gtgtagttga cccccttgag gccgtgacgg atggagacca
     3841 gcccggggca gatgtccggg atcagcatcg ggatgaggag cgggctgacg cgggcggggc
     3901 cgcgctcgaa gagaacctga cactcctttt gaaagacctg catgccgccg atgccggagc
     3961 cgagaacgac cccgaaacgg tcgcggtcgc actgcccgaa atcgatgcct gcatcctcaa
     4021 cgcacttttg cgccgcgatc accccgaact gggtgaagcg gtccatgcgg cgcgcctcct
     4081 tgggctcaat atagtcggtc accacgagat ccctgatctc gcctgctatc cgggtatcat
     4141 aggcggagga atcaaaggcc tcaaaacggg aaatgccgct ctttccagca accagattgt
     4201 cgaacatctg cgcaggggtg tgcccg
//
LOCUS       DAOPWU010000307         4216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21203_length_4216_cov_4.261716, whole genome shotgun sequence.
ACCESSION   DAOPWU010000307 DAOPWU010000000
VERSION     DAOPWU010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21203_length_4216_cov_4.261716"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PKM23_12710"
     CDS             complement(<1..903)
                     /locus_tag="PKM23_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNY92377.1"
                     /translation="MSAAKKLVESISRRHLLRDYIAIIIGAFIMASGIAIFLVDAKVV
                     PGGVSGLAMAFHYLSGDKIPVGMMMWVFNIPLYIWGVRELGKQFGARTFVGFTANSFF
                     IDLLRGQLPLFSGLRLQEHPAIVALRQNDFLFLILIGSVLLGVGLGIIFKFRGSTAGS
                     DVLAAVGQKRWGLKPGMVFMIVDSLVITFAGLVIHYRHLAVDRPALTLTLYAFFLLFV
                     SSHLVDVIIDGFDYARSVMIISSRPEEVSRHIVQDMGRGATAVPARGVYTNETREVLY
                     TVVSPKEIGTLIEVVKEIDPRAFVI"
     gene            1027..1995
                     /locus_tag="PKM23_12715"
     CDS             1027..1995
                     /locus_tag="PKM23_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HNY92378.1"
                     /translation="MIAIRMNSTGDSSVLAPEEAALPQPGAGEVRVKLAAAGVNFIDI
                     YHRTGLYKVPLPYTPGQEGAGTVDAIGPGVEGFQHGDRVAWAMHAGGYAEYAVVPAWK
                     LVQLPAEISFGQGAAVMLQGMTAHYLSHDTFVLQPGVHALVHAAAGGTGQLLVQMARL
                     CGAQVAAVVSTPEKGEIARGCGAGEVLYSGDPEWPQQARAWSGGRGVDVVYDSVGAAT
                     FEGSLAALRVRGMLVLFGQASGPVPPFDPGRLAAAGSLYLTRPVLASYAASAEEVQRR
                     SGDLFAWMGEERLQVVIAREFALADAAAAQDFLHSRRALGKVLLVP"
     gene            2262..>4216
                     /locus_tag="PKM23_12720"
     CDS             2262..>4216
                     /locus_tag="PKM23_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNY92379.1"
                     /translation="MRWKRWWAAVSVLLMAAAGTARSAWLEDVPVLLRQPDGREVQCY
                     ITGDEYFNWVHDGLGFPVLKDSGSGWYIYGERAGGGLAATNHRVGEVDPVRAGLVPGI
                     PLVPEELEQLRRDFQAPALRKAPGLAGEAAPRTGMMQNLVVFIRCSDDPEFTGPISTY
                     TSSFNSSEPGFNSVYNYFREISYQQLGVESHFYPPSGSTIVSYRDSHPRAYYKPVADD
                     PLGYADATERGAREQALIRNALEAVKGQIPAGLNLDGDGDGLIDNMVFIVRGSPSAWN
                     TLLWPHAWALYQYAVSINGKRAYSYSFTLESFVGNGSTHVLVHEMLHTIGFPDLYHYS
                     YDGLNPVGVWDVMASPTAPPQHPGAWCKHKYGGWIPELPLLTENGTYSLQPLAIAQGC
                     ACRIPSPADPAGGEFFVVEYRRRSGSLFDSALPADGLLVYRINQSDPLCYGNGAQPDE
                     VYIFRPDGTPSFDGNLWSAPFSADAGRTLFNQDSNPAPLLADGAPGGIDLFNVGPAGS
                     AITFSLALLPPEAPLRMTPAEGEVDVPLNVVFSWQASAGAHSYHLQLAADPGFTRLLA
                     DQEEIPLLYWPVNELESRTVYYWRIRALSSGGAGEWSGAWRFTTLEANDLPVELLSFS
                     ATARAGGIELEWATATETENYGFEIERR"
BASE COUNT          734 a         1277 c         1418 g          787 t
ORIGIN      
        1 gatgacaaaa gcccggggat cgatctcctt gaccacttcg atgagggtgc cgatttcctt
       61 ggggctaacg acagtgtaga gcacctcgcg cgtctcgttg gtatagaccc cgcgggcggg
      121 gacggccgtg gcgccccggc ccatatcctg gacgatatgg cgggaaacct cctccggccg
      181 ggaggagatg atcatcaccg aacgggcata gtcaaagccg tcgatgatca catccaccag
      241 gtggctggag acgaaaagga ggaaaaaggc ataaagggtc agggtcaggg caggccggtc
      301 gacagcgaga tggcggtagt gaatgaccag accggcgaag gtaatcacca gggaatcgac
      361 gatcatgaag accatgccgg gcttgaggcc ccagcgcttt tggccgacgg cggcgaggac
      421 gtcagagccc gcggtgctgc cgcggaattt gaagatgatg cccaggccga ccccgagcag
      481 tacactgccg atgaggataa ggaagagaaa gtcgttctgg cgcagggcga cgatggccgg
      541 gtgttcctga aggcgcaggc ctgaaaacag ggggagctgt ccgcgcagca gatcgatgaa
      601 aaaagagttg gcggtaaagc cgacgaaggt gcgggcgccg aactgcttgc ccagttcccg
      661 cacaccccag atatagagag ggatattgaa gacccacatc atcatgccga ccgggatctt
      721 gtctcccgag aggtagtgga aggccatggc gaggccgctc actccgccgg ggaccacctt
      781 ggcatcgacg agaaagatgg cgatgccgga ggccatgatg aaggcgccga tgatgatggc
      841 gatataatca cgcaggaggt gcctgcggga gatcgattcg acgagttttt ttgctgcgga
      901 cattggtgct ttcctgatgg ttgggattcg aggggaatgt ttgatattgc acaagaccaa
      961 atttaccatt attttaggat ataatccagc agatttttgc cctgtggaga gcatggagga
     1021 gaaaaaatga tcgccattcg catgaacagc accggggatt catcggtgct ggcgcccgaa
     1081 gaggctgccc tgccccagcc cggtgcgggc gaggtgcggg taaaactggc cgctgcgggg
     1141 gtgaatttca ttgatattta ccatcgcacc ggcctgtaca aggtgcccct gccctacacg
     1201 ccggggcagg agggggctgg cacggtcgat gccatcggcc ccggggtcga gggttttcag
     1261 catggcgacc gggtcgcctg ggcgatgcac gccggcggct atgccgaata tgccgtcgtg
     1321 cccgcctgga agctggtgca gctgccggcg gaaatctctt tcgggcaggg ggccgccgtc
     1381 atgctgcagg ggatgaccgc ccactatctc agccacgaca cctttgtgct gcagcccggg
     1441 gtgcacgccc tggtgcatgc cgccgccggc ggtaccgggc agctgctggt gcagatggcg
     1501 aggctgtgcg gggcgcaggt ggcggcggtg gtctcgacgc cggaaaaggg agagatcgcc
     1561 cgcggctgcg gagccgggga ggttttatat tccggcgatc cggagtggcc gcagcaggcc
     1621 cgcgcctgga gcggggggcg cggggtggat gtggtctacg attcggtggg ggcggcgacc
     1681 ttcgagggca gcctggccgc cctgcgtgtg cgcgggatgc tggtgctctt cggccaggcg
     1741 agcgggccgg tgcccccctt tgatcccggc cgtcttgccg ctgcggggtc gctatacctt
     1801 acgcgtccgg tgctggccag ctacgccgcg tccgcagagg aggtgcagcg gcgcagcggt
     1861 gatctcttcg cctggatggg ggaggagcgc ctgcaggtgg tcattgcgcg tgaattcgcc
     1921 ctcgctgacg cggctgcggc ccaggatttt ctccactccc gccgggccct cggcaaggtg
     1981 ctgctcgtgc cctgagtttt cctctccttt ccctcacttt tggcatgccc gttgcatggt
     2041 acggcagtgc cgggcggcgc attccggcct gaagagagca ttcagacgag taaaatcaat
     2101 gacttggatc tctttcaggc agccgggatg cggcgctgcc gggctgcgga tggggcataa
     2161 tggcaacaca tcggccctcc ggccggtcgt tatgcagggt aaatgggtag ccgaggccac
     2221 accgggtgag gatgcagtgg cggagagggt ggacgggagt gatgcgatgg aagagatggt
     2281 gggcggccgt ctccgtgttg ctgatggcag cagcggggac ggcccggtcg gcctggctgg
     2341 aggatgtgcc tgtacttctg cggcagccgg acggacgcga ggtccagtgc tacatcaccg
     2401 gggacgaata ttttaactgg gtgcacgacg gcctcggctt tccggttctc aaggattccg
     2461 gcagcggctg gtatatctac ggggagcgtg cgggcggcgg gctggccgcg acgaatcacc
     2521 gcgtcgggga ggttgacccg gtgcgtgcgg ggctggtgcc gggcatcccc ctggtgccgg
     2581 aagagctgga acagctgcgg cgcgattttc aggcccctgc cctgcgcaag gcgcccggcc
     2641 tcgccggcga ggctgcgccc cgcaccggca tgatgcagaa tctggtcgtc ttcatccgct
     2701 gcagtgatga tccagaattc accggtccga tttccaccta cacctcctcg ttcaacagct
     2761 ccgagcccgg gttcaactcg gtctacaact atttccgcga aatctcctat cagcagctcg
     2821 gggtcgaatc ccatttctat cccccctccg gcagcacgat cgtttcctat cgggacagcc
     2881 atccgcgcgc ctattacaag cccgtggccg atgatcccct cggctatgcg gatgcaacgg
     2941 agcgcggtgc gcgcgagcag gccctgatca ggaacgccct cgaggcggtc aaggggcaga
     3001 tcccggccgg gctgaatttg gatggagacg gagacggcct catcgacaac atggttttca
     3061 tcgtccgggg cagcccctcg gcctggaaca ctttgctctg gccgcacgcc tgggccctct
     3121 accagtacgc ggtgagcatc aacggcaagc gcgcctattc ctacagtttc accctcgaga
     3181 gctttgtcgg caacggcagc acccatgtac tcgtccatga gatgctgcac accatcggtt
     3241 ttcccgacct ctaccactac agctatgacg gcctcaatcc ggtgggggtg tgggatgtga
     3301 tggccagtcc gacggccccg ccccagcacc ccggcgcctg gtgcaagcac aagtatggcg
     3361 gctggatccc cgaactcccc cttttgacgg aaaacggcac ctactccctg cagccactgg
     3421 ctattgcgca gggctgcgcc tgccgcatcc cctccccggc ggatccggcg ggcggcgagt
     3481 tttttgtcgt ggagtaccgg cgccgatccg gaagtctctt cgattcggcc ctgcccgccg
     3541 acggtttgct cgtctaccgc atcaatcaga gcgatcccct ttgctatggc aacggggcgc
     3601 agcctgatga agtttatatt ttccgcccgg acggcactcc ctcctttgac ggcaatctct
     3661 ggagcgcccc cttcagtgcg gacgccgggc gcaccctttt caatcaggac agcaatccgg
     3721 cccccctgct tgcggatggc gcgcccgggg gcatcgatct gtttaatgtg ggtccggccg
     3781 gctccgccat caccttttcc cttgccctcc tgcctcccga ggcgcccctg cggatgaccc
     3841 cggccgaggg cgaggtggat gtccccctga atgtggtctt ttcctggcag gcgtcggcgg
     3901 gggctcattc ctatcatctg cagctggcgg ccgaccccgg tttcacgcgc ctgctcgccg
     3961 atcaggagga gatccccctg ctttactggc cggtgaacga gctggagtcc cgaacggtct
     4021 attactggcg catccgcgcc ctcagctccg gcggcgcagg ggagtggtcc ggcgcctggc
     4081 gcttcaccac cctcgaggcc aacgacctgc cggttgagct gctctccttt tccgccacag
     4141 cccgggcggg ggggatcgag ctggagtggg cgaccgcgac cgagaccgag aattacggct
     4201 ttgagatcga aaggcg
//
LOCUS       DAOPWU010000308         4208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21271_length_4208_cov_5.056826, whole genome shotgun sequence.
ACCESSION   DAOPWU010000308 DAOPWU010000000
VERSION     DAOPWU010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21271_length_4208_cov_5.056826"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3245
                     /locus_tag="PKM23_12725"
     CDS             <1..3245
                     /locus_tag="PKM23_12725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY92380.1"
                     /translation="ALLEDRDGLVWICTEGRGIVRCHQGESSRLDSEQGLPTSGDSFS
                     TIMEDREGSIWIGGDGLLRIRDNTFTCWGRPEKLPGDYGHSVCADPAGTVCAGFKSGG
                     VALIRDHKFDTIYPCRNLLPNHLTVVLPAREGGFWVGYTNGTVFRITSSSEPIQYTSR
                     TFWGNFALRVLLEDSRGRLWRGMSGLLSCREEGGWWDHWFDRLMRQNSEITAMIELAE
                     DDFLFGTYGDGLRRLTRGRFHQEGIPPELQRDGITLLWRDQDRVLWIGSDNHGLYRFA
                     DGRYASYTEEDGLFAGRIFAILEDDSSNFWCSSNKGVFRVAKSALADFSAGRIAKIPC
                     RVYSQLDGMREAECSGRRQPAGWKSPDGRLWFTSLAGFVSVDPNHLPHNTVPPPVYIE
                     SLRTGDSLYACGAGALRLAARERDLHISYTGLSFAIPERVHFRTRLEGYDKEWRDAGD
                     RREVSYTNLPHGSFTFRVSACNNDGVWNEAGAAQLFVIPPFWYETWWAWLVYVSLAAG
                     ALAWIAQRYYRRLFLRQQLSLQREHTARLEELNEAKSRFFAGISHEFRTPLTLIEAPL
                     EELAGQKGDGKSALYAMMLRNTRRLQGLVEQLLDLARMQSGKMTLQAQPVAVFPLLKM
                     IVASFESLARRKRIKLSLEPLPANLPADPPLTLWLDEEKIEKVLVNLLDNALKYTPGG
                     GKVTVILEGPVQWEKRRCLRISVADSGPGIPEEALPHLFDYFYRYRDENRNSGQGTGI
                     GLALSREIIRLHRGEIEVESSPGRGSCFSVLLPLGREHLRPEEIAGEKLLSEFTPLPA
                     IAEAAFNGEPDLPLPEAEPETPDSRKTLLIVEDNADMRDLLRRLLGSDYRLREAREGN
                     EGLRMALRTPPDLILSDVMMPGMDGYELLRALGADSRTSHIPVLLLTARGGGEERITG
                     LQHGAADYLTKPFDRRELHLRIRNLLDLQERVRESLRQRLTALGSAWPGEAVIAPADE
                     KFMQRAVVLIQEHGGESAFAAQALAQRLGVSRAHLNRKLVALTGMKTSHFIRAIRLQR
                     AAEMLRQRAGSVSEIAYAVGFNHLSYFAACFREQYGCSPSEYNEKQPVKS"
     assembly_gap    3418..3427
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3494..>4208
                     /locus_tag="PKM23_12730"
     CDS             3494..>4208
                     /locus_tag="PKM23_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92381.1"
                     /translation="MKRWHAVLLCCSFLAFLPACEKDSTGPDDVELAQSQETALDLME
                     ATAWEQDASMAKIKYKGKALPFFKREVLTGDIVHYSFRVQVGPGAYDFIGIHRVVKER
                     KVGQPIKTAKNLFLQHGDSKDFTGMFLNGVRTPNVADDFGIAIFLARNDVDVWGIDQN
                     WALVPASVTDFTFMKDWGIENQADNLKLAMEIAQFARKYSGCGSNALNLSGYSSGVLT
                     GYALLNKEAKLAKNKRVVAG"
BASE COUNT          803 a         1281 c         1288 g          826 t
ORIGIN      
        1 gggctctgct cgaggaccgc gacggcctcg tctggatctg cacggagggg cgggggatcg
       61 tacgctgtca tcagggagag agcagcaggc tagattcgga acaggggctg cccacgagtg
      121 gagattcttt ctcgacgatc atggaggacc gcgagggatc gatctggatc ggcggcgacg
      181 gtctgctcag gatccgggat aacaccttca cctgctgggg ccgccctgaa aagctgcctg
      241 gtgattacgg ccattcggtc tgcgcggatc ccgccggcac ggtttgtgcg ggattcaagt
      301 cgggcggcgt ggcgctgata cgcgatcaca aatttgatac tatttatccc tgcaggaatc
      361 tgctgcccaa tcatctgacc gtggtgctcc cggcccgcga ggggggcttc tgggtcggat
      421 acaccaatgg gactgtcttc aggatcacct cttccagtga acccattcag tatacgtcca
      481 gaactttttg gggcaatttt gccctgcgtg tcctgctcga agacagccgc gggcgcctct
      541 ggcgcgggat gagtggcctg ctcagctgcc gcgaggaggg aggctggtgg gaccactggt
      601 tcgaccggct gatgcgccag aattccgaaa tcacggcaat gatcgaattg gcggaggacg
      661 attttctctt cggcacatac ggcgacggac tgcgccgcct cacccgcggg cgcttccacc
      721 aggagggcat cccccccgag ctgcagcgcg acggcatcac cctcctctgg cgcgatcagg
      781 atagagttct ctggatcggc tcggacaatc acggcctcta ccgctttgcg gatgggcgtt
      841 atgccagcta caccgaagag gatgggcttt tcgccggccg catcttcgcc atcctcgagg
      901 acgacagctc aaatttctgg tgcagcagca acaagggggt cttcagggta gctaaaagtg
      961 cccttgcgga tttttcggcg ggccggattg cgaagatccc ctgccgggtc tatagccagc
     1021 tcgatggcat gcgcgaggcg gagtgcagcg ggcggaggca accagcggga tggaaaagcc
     1081 ccgacggccg cctctggttc acctccctcg ccggctttgt ttcggtcgat cccaaccacc
     1141 tgccgcacaa cacagtccca ccccccgttt acatcgagag cctgcggacc ggcgactcgc
     1201 tttatgcctg cggcgccggg gctctgcggc tggccgcccg cgagcgcgat ctccatatca
     1261 gctacaccgg tttgagcttc gccattcccg aacgggtgca ctttcgcacc cggcttgagg
     1321 gctatgacaa ggagtggcgc gacgccggcg accgccgcga ggtctcttat accaatcttc
     1381 cgcacgggag tttcaccttc cgggtgtcgg cctgcaataa cgacggcgtc tggaacgaag
     1441 cgggagccgc ccagctcttc gtcattcccc ccttctggta cgaaacctgg tgggcctggc
     1501 tggtctatgt cagcctcgct gcgggcgccc tggcgtggat cgcacagcgg tattaccgcc
     1561 gtctcttcct caggcagcag ctctccctgc agagggagca cactgccaga ctggaagaac
     1621 tgaacgaggc caaatcccgc tttttcgccg gtatctccca cgaattccgc accccgctca
     1681 ccctgatcga ggcccctctc gaggagctgg ccgggcaaaa gggcgacggc aaaagcgcac
     1741 tctacgccat gatgctgcgc aacacccggc ggctgcaggg gctggtcgag cagctgctgg
     1801 acctggcgcg gatgcagtcg ggcaagatga ccctgcaggc gcagccggtc gcggtttttc
     1861 ccctcctgaa aatgatcgtc gcttcctttg aatccctggc ccgccgcaag cggatcaagc
     1921 tctcgctgga accgctgccc gccaatctcc cggcggaccc gcccctgacc ctctggctcg
     1981 acgaggagaa gatcgagaag gtgctcgtca atctcctcga taacgccctg aaatacactc
     2041 ccggcggcgg caaggttacg gtaatcctgg aggggcccgt acagtgggag aaacgccgct
     2101 gcctgaggat ttcggtcgct gacagcgggc cgggcattcc ggaggaggcc ctgccccatc
     2161 tcttcgatta tttttatcgc taccgggacg agaacagaaa ctccgggcag ggaaccggca
     2221 tcggacttgc cctcagccgt gagatcatca ggctgcaccg cggcgagatc gaggtcgaga
     2281 gttcaccggg ccggggcagc tgtttcagcg ttctcctgcc cctcggcagg gaacacctgc
     2341 ggccggagga gatcgccgga gaaaagcttc tctccgagtt taccccgctg ccagccatag
     2401 ccgaggccgc tttcaatggt gagccggacc ttcccctgcc ggaggctgag cccgagacgc
     2461 cggacagccg caagaccctt ctcatcgtcg aggacaacgc cgatatgcgc gacctgctgc
     2521 gccgcttgct gggcagcgat taccggctca gggaggccag ggagggaaac gagggcttgc
     2581 gcatggcgct gcgcacgccg ccggacctca tcctcagcga tgtcatgatg cccgggatgg
     2641 atggctacga gctgctgcgc gccctgggcg cggacagccg gaccagccat atacccgtgc
     2701 tgctgttgac cgcccgcgga ggaggggagg agagaatcac gggcttgcag catggtgcag
     2761 ccgactatct caccaagccc ttcgaccgca gggaactgca tctgcgcatc cgcaacctgc
     2821 tcgatttgca ggagcgggtc cgggagagct tgcggcagcg gctcactgcc ctcggcagcg
     2881 cctggccggg agaggctgtg atcgccccgg cggacgaaaa attcatgcag cgggcggttg
     2941 tgttgattca ggagcacggc ggagagtccg cctttgcggc ccaggccctg gcgcagcggc
     3001 tgggggtcag ccgggcccac ctcaaccgca agctcgttgc ccttaccggc atgaagacca
     3061 gccacttcat ccgtgccatc cggctgcagc gtgccgccga aatgctgcgc cagcgcgccg
     3121 gctcggtcag cgagatcgcc tacgccgtgg gattcaacca cctctcctac ttcgccgcct
     3181 gcttcaggga gcagtatggc tgctcgcctt ctgaatacaa cgaaaaacag ccggtaaaat
     3241 cctgataagg gcttttttat cgcatttctg ttaaagctga tcatttccgt gttagctata
     3301 ataataccag tttactatta ttggtaaacg atgggggaga aactacgggg aaatccgcag
     3361 tacggttggg atgcgcaggg ggggcatccc aacacccttt tctttccgct gtgtgttnnn
     3421 nnnnnnngca tcccaacctt cttttcaatt tgtcttgttc atccaacaca tcgttacaag
     3481 agaggggggc gctatgaaaa ggtggcatgc ggtccttctg tgctgcagtt ttctggcctt
     3541 tttaccggcc tgcgagaagg attcgactgg acctgatgat gtcgagttgg ctcaaagcca
     3601 ggagacagcc ctggatctga tggaggcgac ggcctgggag caagacgcct cgatggcgaa
     3661 gattaaatac aaaggcaagg ccctgccttt ctttaaacgc gaggtgctga ccggagacat
     3721 tgtccattac tctttccggg tccaggtcgg cccaggcgcc tatgatttca tcggcatcca
     3781 ccgtgtcgtc aaggagagaa aggttggtca gccgatcaag accgctaaaa acctcttcct
     3841 gcagcatggc gattccaagg atttcaccgg catgtttctc aacggggtgc gcactcctaa
     3901 tgtggcggac gatttcggca tcgccatttt tcttgcccgc aacgatgtcg atgtctgggg
     3961 cattgaccag aactgggctc tggtgcccgc aagtgtgacc gatttcacct tcatgaagga
     4021 ttggggcatc gagaatcagg cggacaatct taaattggcc atggagatcg cccagtttgc
     4081 gcgcaaatac tccggctgcg gcagcaatgc cctcaatctt tcgggctaca gcagcggcgt
     4141 actgaccggc tacgccctgc tgaacaagga agctaaactg gccaagaata aacgggtggt
     4201 ggcaggct
//
LOCUS       DAOPWU010000309         4196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21354_length_4196_cov_5.200435, whole genome shotgun sequence.
ACCESSION   DAOPWU010000309 DAOPWU010000000
VERSION     DAOPWU010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21354_length_4196_cov_5.200435"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2081
                     /locus_tag="PKM23_12735"
     CDS             <1..2081
                     /locus_tag="PKM23_12735"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNY92382.1"
                     /translation="EQIWGLEIYRDLARRDETALWQHIPKDASGIVHFFGDLHGLKNL
                     AQPKRVELLPYTVSDLSTGRPEPDNPFAPGRTGHFKGGLDGKAGVTSDLTMDFTINPD
                     FGQVEADPSEVNLTAFETFYEEKRPFFIEGKNIFDYRLVMGDGDLSMDQLFYSRRIGR
                     APQREIETEDDEYLRSPANTSILGAFKLSGKTRSGLSVGVLDAITQKEEAEIRRGEAS
                     QREAVEPLSNYFVSRLQKDYSAGATSVGLMMTGTHRRISIDQLNFLNRAAYTGGIDLR
                     HAWNKQNWLVDFRSAFSHIRGHKEALLEAQTSSARYFQRPDADHVQVDSSRTTLSGTG
                     GSLSFGKVGGGHWRFLVMGIWRTPGLELNDLGYMRQADQLIQVFWAQYRTLKPYGKLR
                     ELYANVNQWQFWNFDGEFIGRGANCNVNLTFTNSWHAGGGISHETRWLGLSALRGGPA
                     IYYPASTNIWYNLSSDDRKAVSFFGRGQNYHNEDGITRSNYYSIGLEARPSAAFSLSL
                     SPSFSYGTEDLQYVDTIETGRGPRYIMGRIDQKNLAMVVRFDYCLTPNLSVQYYGQPF
                     IAAGNYTHFKSVTRPRASVHTDQFKTFSAEEIEYDGENDQYAIDEDSDGQEDFRFDKP
                     DFNFRQFRSNLVLRWEYRAGSTLFLVWSQGRSNSIEEGRFSYRHDMRELFNVYPDNVF
                     LVKCNYWFSL"
     gene            2152..2823
                     /locus_tag="PKM23_12740"
     CDS             2152..2823
                     /locus_tag="PKM23_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92383.1"
                     /translation="MKRMLFFSSTAGLLLAAVVTASGAVQLGISQSDDKALAFRSTPG
                     IMLGNSLAWHPSARISLITELNLHYAQIALKEQRVWHYPGMLSLYDLTAKALYFEIPA
                     LLGYTLPLRGSWVQFYAGPSLHLCLSGAVQRTRIRVIADEQTWGGDNPLPDYDVSFIE
                     DPGPDLALMNSAVGLNLGLWTRWSEWLIGFRYTCSRLHELDAVRLNSPFHTFSLMISF
                     QPTGD"
     gene            complement(2855..3025)
                     /locus_tag="PKM23_12745"
     CDS             complement(2855..3025)
                     /locus_tag="PKM23_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA binding domain-containing
                     protein"
                     /protein_id="HNY92384.1"
                     /translation="MAGEEKKSFLLRIPPKLWAELEKWAADELRSVNGQIEFILRQAV
                     ARRKHNGDKEPS"
     gene            complement(3028..4068)
                     /locus_tag="PKM23_12750"
     CDS             complement(3028..4068)
                     /locus_tag="PKM23_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNY92385.1"
                     /translation="MSTEAVKPASIEKEVGAINGWPMLFINLIILFSGPAMIVWASVT
                     GAQGRLGAGAIVSGVLFGAFLIILGIIMLSGHIALQPNQARVLILFGAYKGTIRKSGF
                     YWANPFYSRTRAKIPVQSAGAPSHGSGKGAAVTTAVNATTGGILYQPLSAKISLRAHN
                     FNSDKLKVNDRRGNPIEIAAVVVWRVQDTAQAAFDVEDYESYVQVQAESAIRSIASLY
                     DYDHGEEHEVTLRNGGDEVAAELTKELQARLHKAGVVVDEARITHLAYAPEIAQAMLR
                     RQQAEAVIAARQKIVHGAVSMVEMALQDLAEKEVVKLDEERKAAMVSNLLVVLCSETA
                     STPVINTGTLYS"
BASE COUNT          843 a         1244 c         1176 g          933 t
ORIGIN      
        1 cggaacagat ctgggggctg gagatctatc gcgaccttgc gagaagggat gaaaccgcac
       61 tctggcagca tatccccaag gacgcctcag gaatagtcca cttttttggt gatctgcacg
      121 ggctgaagaa tctcgcccag cccaaacgcg ttgaactgct cccctacacc gtcagcgatc
      181 tgagtacggg ccggccggaa ccggacaatc cgtttgcgcc cggccgcacc ggccacttca
      241 agggcggtct ggacggcaag gcgggcgtca cctcggacct gaccatggat ttcaccatca
      301 atcccgattt cggccaggtc gaggccgatc cctcggaggt caacctcacc gcctttgaaa
      361 ccttttatga ggaaaagcgc cccttcttca tcgagggcaa gaatattttc gattaccgct
      421 tggtcatggg cgacggcgat ctttccatgg accagctttt ttactcccgc cgcatcggac
      481 gtgcgccgca gcgggaaata gagacggaag atgatgaata cctgcgctcg cccgccaaca
      541 cctcgatcct gggcgccttc aaactcagcg gcaagaccag aagcggcctc tcggtcggcg
      601 tgctcgatgc cattacccaa aaagaggagg cagagatccg ccggggagag gcctcgcagc
      661 gggaggcggt tgagcctctg agcaactatt ttgtcagccg cctgcaaaag gattacagcg
      721 ccggggccac ctcagtcggg ctgatgatga cgggcaccca ccggcgcatc agtatcgacc
      781 agctcaactt tctcaatcgc gccgcctata ccgggggcat cgatctgcgc catgcctgga
      841 acaagcagaa ctggctggtc gatttccgta gcgccttcag ccatattcgc ggccacaagg
      901 aagccctgct cgaagcgcag acctcctcgg cgcgctattt tcagcgtccc gatgccgatc
      961 acgtccaagt ggattcctcg cgaaccacgc tttctggaac cggaggctcc ttaagtttcg
     1021 gcaaggtcgg cggcggccat tggcgattcc tcgttatggg catctggcgt acaccagggc
     1081 tcgaattgaa tgatctgggt tatatgcgtc aggccgatca gctcatccag gttttctggg
     1141 cgcagtatcg caccctgaaa ccgtacggaa aactgcgcga gttatatgcc aatgtcaatc
     1201 aatggcagtt ctggaatttt gatggtgaat tcatcggacg gggcgcaaac tgcaatgtga
     1261 atctgacctt caccaacagc tggcatgccg ggggtggcat cagccatgag accaggtggc
     1321 ttggcctctc tgccctgcgc gggggaccgg cgatctatta tccggcgagc acaaatatct
     1381 ggtacaatct ctcctcggac gaccgcaaag cggtaagctt tttcggccgc gggcagaatt
     1441 atcacaatga ggacggcatc acccgttcga actactattc tatcggtctg gaagcgcgtc
     1501 cctcagcggc ctttagtctc tcgctctccc cttcattctc ttatggcacg gaggatctgc
     1561 agtacgtcga taccatcgag accggccgcg ggccgcgcta catcatgggc cgcatcgatc
     1621 agaaaaacct ggccatggtg gtgcgcttcg actattgcct gacccccaat ctctcggttc
     1681 aatattacgg acagcccttt atcgccgcgg gaaattacac ccatttcaag tccgttaccc
     1741 ggcctcgtgc gagcgtgcac actgatcagt ttaaaacttt cagcgctgag gaaatagagt
     1801 acgacgggga gaatgatcaa tatgccatcg acgaggactc ggatggccag gaggatttcc
     1861 gtttcgacaa acccgatttt aatttccgcc agttccgttc gaatctggtg ctgaggtggg
     1921 agtaccgggc cggttcgacc ctttttctgg tctggtcgca ggggcgcagc aattccatcg
     1981 aggagggccg tttctcatat cgccacgata tgcgcgagct gttcaatgtc tatccggaca
     2041 acgtctttct ggtcaagtgc aactactggt tttcgttatg atcttgccat ccgcagctca
     2101 gactaggctg cggatggcca caccctgcat ggcgcaggga aggggggaag aatgaaaagg
     2161 atgctttttt tctcttctac agccgggctg ctgctcgcag cggtggtcac ggcttctggt
     2221 gccgtacaac tgggcatttc acagtcggac gataaagccc tcgctttccg ctccaccccc
     2281 ggcatcatgc tgggcaattc cctggcctgg catccatccg ccagaatatc cctgatcacc
     2341 gaactgaacc tccattatgc ccaaatcgca ctcaaggagc agcgggtctg gcactatccc
     2401 ggcatgctct ctctttatga tctgacggcg aaggcactgt atttcgagat tccagctctc
     2461 ctgggataca ccctcccgct gcgcggctct tgggtgcagt tctatgccgg cccctccctg
     2521 catctgtgcc tttccggagc ggttcagcgc accaggatca gggtcatagc tgacgagcag
     2581 acctggggtg gagataaccc tttacccgac tatgatgtat cgttcatcga ggatcccggc
     2641 cctgaccttg cattgatgaa ttctgcggtc ggcctgaatc tgggactctg gacgcgctgg
     2701 tcagaatggc tcatcggatt tcgctacacc tgcagccggc tccatgagct ggatgcggtc
     2761 aggctcaaca gcccctttca taccttcagt ctgatgatct ccttccagcc gacgggagat
     2821 tgaagatctg tacgccgaac ctctccgcga gaattcagga tggttcttta tcgccgttgt
     2881 gcttgcgccg cgccaccgct tgccgcagga tgaattcgat ctgtccattg acgctgcgca
     2941 gttcatcggc ggcccatttc tccagctccg cccacaattt cggagggatg cgcagcagga
     3001 acgatttttt ctcttctccg gccatgatca actgtagagc gtgccggtgt tgatgaccgg
     3061 ggtggaggcc gtttcgctgc acaggacgac gagcagattg ctgaccatcg ccgccttgcg
     3121 ctcctcatcc agcttcacca cttccttttc cgccagatcc tgcagcgcca tctccaccat
     3181 gctcaccgcg ccgtggacga ttttctgccg cgccgcgatg accgcctccg cctgctggcg
     3241 gcgcagcatc gcctgggcga tctcgggggc ataggcgaga tgagtgatgc gggcttcgtc
     3301 gaccactacc ccggccttgt gcagccgcgc ctgcagctcc ttggtcaatt ctgctgccac
     3361 ctcgtcgccg ccgttgcgca gggtgacttc atgttcctca ccatgatcat agtcgtagag
     3421 actggcaatg ctgcggatcg ccgactcggc ttgcacctgc acatagcttt cataatcctc
     3481 gacatcaaac gccgcctggg cggtatcctg cacgcgccac accaccactg cggcgatttc
     3541 gatggggttg ccgcgccggt cgttgacctt gagcttgtcg ctgttgaaat tgtgggcgcg
     3601 cagggaaatc ttggcgctca ggggctgata gagaatgccg ccggtggtgg cattgactgc
     3661 cgtggtcacg gctgcaccct tgcctgaccc gtgtgatgga gctcccgcgg actgtaccgg
     3721 tatcttggcg cgggtgcgcg aataaaaggg attggcccaa tagaagccgc tcttgcggat
     3781 cgtccccttg taagcgccga agaggatgag cacacgggcc tggttgggct gcagtgcgat
     3841 gtggccgctg agcatgatga tacccaggat gatgagaaaa gcaccaaaga gcacgccgct
     3901 cacgatggcg ccggcgccga gccgcccctg cgcgccggtg acggaggccc agacgatcat
     3961 agccgggccg gaaaagagga tgatcagatt gatgaagagc atcggccagc cattgatggc
     4021 gcctacttct ttttcaatgc tcgcgggttt gacggcttcg gtggacatag acacctccat
     4081 tatatgatat aatagtgata tcaaaaagct ataagaaata tatcgaatcc cggcatgatt
     4141 gtcaagggat tttttaggac tgctgcagcg gaattgttcg ggaagcgcat tttttt
//
LOCUS       DAOPWU010000310         4189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21397_length_4189_cov_4.237784, whole genome shotgun sequence.
ACCESSION   DAOPWU010000310 DAOPWU010000000
VERSION     DAOPWU010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21397_length_4189_cov_4.237784"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKM23_12755"
     CDS             <1..663
                     /locus_tag="PKM23_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNY92386.1"
                     /translation="WCDKLGLLVWQDMPSGDQSISPDQVDLVRTPASAAQYYLELGRM
                     VANLFNHPSIVIWVPFNEGWGQFDSGKVVEFIRGLDSTRLVNQASGWTDRGGGDVVDW
                     HRYPGPDSPKPEPLRAAVLGEFGGLGLKSAGHMWTEENWGYQKMEDAAGLQKRYEQLW
                     TRVWELQETAGLSAAVYTQTTDVETEANGLMTYDREIIKQDIARAPLLHSGRLPMLPL
                     AR"
     gene            695..2656
                     /locus_tag="PKM23_12760"
     CDS             695..2656
                     /locus_tag="PKM23_12760"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HNY92387.1"
                     /translation="MKYSGFVLSVLLFLAGTAFAGVLALDSPDKNITISVDIAENIRY
                     AVAWRGQEVIRPSEISLLLDRERHLGDQPRLQKSRRAAIDRMLEPVVRQKSARIRDHY
                     NEMVLDFKGGYSLIFRAYDNGVAWRWVTRLPGRVKVLYEQAVFDFAADHTLYFGEEPS
                     FYSHQERHYLPVRLKEVTSARQAVAPVLIDGGEGPRVLISEADLCDYPGLWFTGSDQR
                     PTSLVGRFPHFCLTDTVTSDRDVKPLTRAPWIAETAGTRSYPWRLLVIAGRDADIVSN
                     SLVYQLSQPEKIADPSWIKPGKVAWDWWNALNIYGVDFKAGLNTATYKHYIDFAAEYG
                     IEYVILDEGWYQLGDVLSVHPEINMDELLAHARERNVGIILWVTWKSLLDKLEPALDR
                     FAQWGVKGIKVDFMQRDDQWMVTYYWRIAEEAARRHLLVDFHGAYKPAGLERAWPNVL
                     TREGVKGLENVKWSREVTPEHDVTLAFTRMAVGPMDYTPGAMVNATPDQFQPVFEMPM
                     SMSTRCHQMAMYVVYESPLQMLADSPSRYRREEECTRFIAGVPAVWDESLVLEARIAD
                     YLLLARRSGGIWYAGAMTDGTGRELVLDLSFLPVGSYTMTLFSDGPNAGRAAMDYTST
                     TRLVTAGERIPLKLAPGGGAAARFEPAVE"
     gene            2762..>4189
                     /locus_tag="PKM23_12765"
     CDS             2762..>4189
                     /locus_tag="PKM23_12765"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNY92388.1"
                     /translation="MKKLIQLFLLLSMASLFLLCAKEEAPYVVKVAKQVIPSVVFEKK
                     FRSSPEFRENVIYNIDVLKEFIDDNYVDNLLFEAEGFARGLERDSVVTARLFIEKGRI
                     LTRPKGPLYAKIVPAEITVTDEEVRADYESSRFEYKIADILFKNKARADSVHALLQKG
                     ADFADLARKYSQDGRSRESGGEVPKYLRWGTMGYAADAALRSMEPGEITPPLVTLFGI
                     HIIKVIDKRPWAAPPFEDVHPRIRNQIKAKKLNQLTEAYSISLYDKYNFRVSSGVLPE
                     VFAHYRTEKGVGRLDLAAMPKKLQKARVAEWKGGSWDVRKLASQLATAPSPLLFPWNS
                     VDDVEDFVRKSAVAELMLAEAEALGLDDSPAFKSEYAALRNQIVRTRCREILAPQIRT
                     LTEEEVQAFYEEQRERLGEVSLAEVRPTLEGQLLLNKNRELRKQAAKQLRGKYPIFYN
                     EAELRRIVLSLGSGGDAVAHQKEEAD"
BASE COUNT          818 a         1310 c         1246 g          815 t
ORIGIN      
        1 tggtgcgaca agctcggcct gctcgtctgg caggatatgc ccagcggcga ccagtccatc
       61 agccccgacc aggttgattt agtgcgtact ccggcttcgg ctgcccagta ctatctcgag
      121 ctggggcgca tggtcgccaa cctcttcaac catcccagta ttgtcatttg ggtccccttc
      181 aatgaggggt gggggcaatt cgactcgggc aaggtggtcg agttcatccg cggccttgat
      241 tccacccggc tggtgaacca ggctagcggc tggacggacc ggggcggcgg cgatgtcgtc
      301 gactggcacc gctatccggg gcctgactcc cccaaacccg agcccctgcg cgccgctgtc
      361 ctcggtgaat tcggcggcct tgggctcaag agcgccggac atatgtggac cgaggaaaac
      421 tggggctatc aaaagatgga agatgcagcc ggtctgcaga aacgttatga gcagctctgg
      481 accagggtct gggagctgca ggagacggcc ggcctcagcg ccgccgtcta tacccagacc
      541 accgatgtcg agaccgaggc taacggcctc atgacctatg accgggagat catcaagcag
      601 gatatcgcgc gggcgccgct gctccacagc ggccggctgc ccatgctgcc gcttgcccga
      661 tgattcccct cattccgtgg gaggatgtga acctatgaag tattcggggt ttgtcctgtc
      721 ggtcctgctg tttttggcgg ggaccgcgtt tgcgggggtg ctggcgctgg attcccccga
      781 taaaaacatc accatcagcg tcgatatcgc cgaaaacatc cgctatgcgg tggcctggcg
      841 cggccaggag gtgatccgcc cctccgaaat ttcactgctc ctcgaccggg aacgccacct
      901 tggcgaccag ccgcgcctgc aaaagagccg gcgcgctgcc atcgatcgca tgcttgaacc
      961 ggttgtccgt caaaagagcg cccgcatccg cgaccactat aacgaaatgg tgcttgactt
     1021 caagggcggc tacagcctca tcttccgagc ctacgacaac ggcgtggcct ggcgctgggt
     1081 gacccgtttg cccggaaggg tcaaggtgct ctatgagcag gccgtctttg atttcgccgc
     1141 agaccacacc ctctatttcg gtgaggagcc cagtttttac agccatcagg aacgccatta
     1201 tctcccggtc cgtctcaagg aggttaccag cgcccgtcag gcggtcgccc cggtgctgat
     1261 cgatggcggc gagggcccca gggtgctgat cagcgaggcg gatctctgcg attatccggg
     1321 gctctggttc accggcagcg accagcggcc gacctccctg gtcggccgct ttccccattt
     1381 ctgcctgacc gacacggtca cctcggaccg cgatgtcaag cccctcaccc gcgccccctg
     1441 gatcgccgag accgccggca cgcgcagcta tccctggcgc ctgctggtca tcgccgggcg
     1501 cgatgccgat atcgtcagca acagtctggt ctatcagctc tcgcagccgg aaaagatcgc
     1561 cgatccttcc tggatcaagc ccggcaaggt ggcctgggac tggtggaacg ctctcaacat
     1621 ttacggcgtc gatttcaagg ccggcctcaa caccgccacc tacaagcact atatcgattt
     1681 cgccgctgag tatggcatcg aatatgtcat tctcgatgag gggtggtatc agctcggtga
     1741 tgtgctctcc gtccatcccg agatcaatat ggatgagctg ctggcccacg cccgcgaacg
     1801 caatgtgggc atcatcctct gggtcacctg gaaaagcctg cttgacaagc tggagccggc
     1861 cctggaccgc tttgcccaat ggggcgtcaa gggcatcaag gtcgatttca tgcagcggga
     1921 tgaccagtgg atggtcacct attactggcg catcgccgaa gaggcggccc gccgccacct
     1981 gctggtcgat tttcacggcg cctacaagcc ggcggggctg gaacgcgcct ggcccaatgt
     2041 gctcacccgc gagggggtta agggtctgga gaatgtgaaa tggagcaggg aggtgacccc
     2101 tgagcacgat gtcaccctgg ccttcactcg catggcagtc ggacctatgg attacacccc
     2161 cggggccatg gtcaacgcca cgccggatca gttccagcct gttttcgaaa tgccgatgag
     2221 catgtcgacc cgctgccatc agatggcgat gtacgtcgtc tatgaaagcc cgctgcagat
     2281 gctcgccgac tcgccgagcc gctaccgccg cgaggaagag tgcacgcgtt ttattgccgg
     2341 cgtgcccgcg gtctgggacg aaagcctcgt gctggaggcc cgcatcgccg attatctcct
     2401 gctcgcccgg cgcagcggcg ggatctggta cgcgggtgcg atgaccgacg gaaccggacg
     2461 cgagcttgtc ctcgacctct ccttcctgcc ggtggggagc tataccatga ccctgttcag
     2521 cgacggcccc aatgccggac gcgccgccat ggattacacc agcaccaccc ggctggtcac
     2581 ggccggcgag cggattccgc ttaagctcgc acctggaggg ggggcggcgg cgcgttttga
     2641 gccggccgtg gagtaatgca aatcaccgcg gcaccgccct ggtggcaata attactgttt
     2701 ttattcatat aatttttgta tctttaaggt caatactacc ccaaaacctg cgagtgtgtg
     2761 catgaaaaaa ctgatccaac ttttcctcct gctctccatg gcctccctgt ttctcctctg
     2821 tgccaaagaa gaggcaccct atgtagtcaa ggtggccaaa caggtcatcc cctcggtcgt
     2881 ctttgagaag aaatttcgca gcagccccga gttccgcgaa aatgtcatct ataatatcga
     2941 cgtactgaag gagttcatcg acgataatta tgtcgacaat cttctcttcg aggcggaggg
     3001 ctttgcgcgc ggcctcgagc gggacagcgt ggtgacggcc cgcctcttca tcgaaaaggg
     3061 gcgcattcta acccggccca agggacctct gtatgccaaa atagtccccg ccgagatcac
     3121 ggtgacggat gaggaggtcc gggctgatta tgaatcctcc cgtttcgaat acaagatcgc
     3181 cgatatcctt ttcaagaaca aggcccgggc ggacagtgtc cacgccctcc tgcaaaaggg
     3241 ggctgatttt gccgatctgg cccggaaata ttcccaggat ggccgctcgc gtgagtccgg
     3301 cggcgaggtg cccaaatatc tgcgctgggg aaccatgggc tatgcagccg atgccgcctt
     3361 gcgcagcatg gagcccggtg aaatcacccc gcccctggtc accctcttcg gcattcatat
     3421 catcaaggtc atcgacaagc gcccctgggc ggctccaccc ttcgaggatg ttcatccccg
     3481 catccgcaat cagatcaagg ccaaaaagct caaccagctc actgaagcct attcgataag
     3541 cctctacgac aagtacaatt tcagagtctc ctccggcgtg ctgcccgaag tcttcgctca
     3601 ctaccggacc gagaaaggag tgggccgtct ggatctcgcc gccatgccca aaaagctgca
     3661 aaaggccagg gtggcggaat ggaagggggg aagctgggat gtgcgcaagc tggcttcgca
     3721 gctcgccacc gccccgtcgc ccctgctctt tccctggaac tcggtggacg atgtcgaaga
     3781 tttcgtccgc aagtcggcgg ttgccgaact gatgctcgcg gaggctgaag cgcttggact
     3841 cgacgactcc ccggccttca aatcggaata cgcggcactg cgcaaccaga tcgtgcgcac
     3901 ccgttgccgc gagattctcg ccccacagat tcgcaccctc acggaggagg aggtccaggc
     3961 cttctatgag gaacagcggg agaggctcgg cgaggtcagc ttggccgagg tgcggccgac
     4021 cctggaaggt cagctcctgc tcaataaaaa ccgcgagctg cgcaaacagg cggccaagca
     4081 gttgcgcggc aaatacccca ttttctacaa cgaggcggag ctgcgccgca tcgtcctttc
     4141 gctgggctcc gggggagatg cggtcgcaca ccagaaggaa gaggctgat
//
LOCUS       DAOPWU010000311         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21456_length_4180_cov_4.089697, whole genome shotgun sequence.
ACCESSION   DAOPWU010000311 DAOPWU010000000
VERSION     DAOPWU010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21456_length_4180_cov_4.089697"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PKM23_12770"
     CDS             complement(<1..537)
                     /locus_tag="PKM23_12770"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HNY92389.1"
                     /translation="MREKGWRNDGRAKVTGGAKFTGDLKFADMLHAVPVYADYIHALL
                     TGINTAEAEKSPGVVRVLTYRDVPGANRFGQIIRDFCIFADDRIRCHGDVVAVVVAET
                     RAQALAAAALVRVEAEPLPALLDPEKAMQPDAILIHESHGSNIINTHRVRSGDTEKGF
                     AAADLILEKTYTTPFVEHA"
     gene            complement(534..1934)
                     /locus_tag="PKM23_12775"
     CDS             complement(534..1934)
                     /locus_tag="PKM23_12775"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNY92390.1"
                     /translation="MKISIQNGTLVLDDRLKQADILIDGERIAAIAPRSTLPPADLTV
                     DAASSLVFPGFIDLHTHLDDVIGGRYLADTWHSGSQIAVQNGITSLCSFITQGSDESL
                     GTAIARAQKKAAGQSFCDYTWHLTPTRFDDAGWREIEAAIGAGFITFKFYTTYREAGL
                     YSSYTELEERIGRLHSLGGRVLVHCEDDAILQRARQGETAADRDTRWPESACAAVDWS
                     DPRSHARVRPAEAEVTAIQRLVEIARRTGARLHIVHVSTPEGVAAIRQAENHRITCET
                     CPHYLFLDESWLGRPDGRRWICAPPLRAEESRRKLAEMVRAGGADCMATDHCAFTRAD
                     KESGGGDIRRTPGGVAGIGALVPLACKLFEGNDAEANLPAISRLLSAHPAQILGLYPR
                     KGCLQAGADADLVVLRSGGPERSIRSTLADAFETYPGMSTTLSVRALFRRGWLIVENG
                     EILEQDIPGGTWLCQK"
     gene            2486..>2878
                     /locus_tag="PKM23_12780"
     CDS             2486..>2878
                     /locus_tag="PKM23_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92391.1"
                     /translation="MNKSFLFFLLICLVPFIVTGQNMLANAGFEDGDADANGIPDGWI
                     GYAQTGASMQLYNEAATAHSGQYWAKCTSTNGGYYLLYQNTFPAAEGDVWEFSSFIKD
                     ISPAYPGAVFAALKISAKNAAGGTFKAWE"
     assembly_gap    2879..2978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2979..>4180
                     /locus_tag="PKM23_12785"
     CDS             <2979..>4180
                     /locus_tag="PKM23_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92392.1"
                     /translation="ANGIPDGWIGYAQTGASMQLMNDAATAFSGSWWAKCTSTNGGYY
                     LLYQQTFPATKGEVWKFSSFIKDLSPAYPGADYVALKISAKNANGGTFQAYEVFQTGL
                     TSYWKDYSNTQTMPEGTHFIQAVIVVHGADGAPEATYGFDDVRLELVHEMTNNPGFED
                     GDSNGDQIPDGWLGGGTAGKTHMETIKDSTAHSGQFWTKVNVDSGDGYYVLYQAGFPA
                     NPGEIWSLRSFIKNISPDSAGDFAFLKISAKNANGSNVKVCEVRQAGVSRQWKDYHNT
                     QTMPLGTTLIQPVLVVKKSAIDSAAFSLVASYAFDDVRVAKLGVADIMAPTEPGAVSV
                     ENGAKPNTNSVTWMDVAGETGETYALYASQSPITKVTDAGVEVLATAIAKGTQKYEHS
                     LRNPYTDSL"
BASE COUNT          846 a         1184 c         1202 g          848 t
ORIGIN      
        1 ggcgtgctcg acgaacgggg tggtataggt tttctccagg atcagatcgg ctgcggcaaa
       61 ccccttttca gtatccccgc tgcggacgcg gtgggtgttg atgatgttgc tgccgtggct
      121 ctcgtggatg aggatcgcgt ccggctgcat cgccttttct ggatcgagca gggcgggcag
      181 gggctcggcc tcaacgcgca ccagcgccgc ggcggccagg gcctgcgccc gggtctcggc
      241 gacgaccaca gcgacgacat cgccgtggca gcggatgcgg tcgtcggcaa aaatgcagaa
      301 gtcgcggatg atctggccga agcggtttgc gccgggcaca tcccggtagg tcagcacccg
      361 gaccactccg ggactctttt ccgcctccgc cgtgttgatt ccggtcagca aggcatggat
      421 gtagtcggca tagaccggca cggcatggag catgtcggcg aatttcagat cgccggtgaa
      481 tttggcccct ccggtcacct tggcgcggcc gtcgttgcgc cagccctttt ccctcatttc
      541 tgacacagcc atgtacctcc gggtatatcc tgctcaagaa tctccccgtt ctccacgatg
      601 agccagccgc gccgaaagag cgcccggacc gacagggtgg tgctcatacc gggataggtt
      661 tcaaaggcat cggccagggt ggagcggatg gaccgctccg gtccgccgct ccggagcacg
      721 accagatcgg catcggcgcc ggcctgaagg caccccttgc gcggatagag cccaaggatc
      781 tgcgcgggat gggccgagag caggcggctg atggccggca gattcgcttc cgcatcattt
      841 ccctcaaaaa gtttgcacgc cagggggacc agggcgccga tgccggctac accacccggg
      901 gtgcggcgga tatcgccgcc tccggactcc ttgtccgccc gggtgaaagc gcagtggtcg
      961 gtggccatgc agtcggcccc accggcgcgg accatctccg ccagcttccg tctcgattcc
     1021 tccgcacgca gcggcggcgc gcaaatccag cgccggccat ccggccgccc cagccagctc
     1081 tcgtcgagga aaagatagtg cgggcaggtt tcgcaggtga tccggtgatt ctccgcctgc
     1141 cgtatcgcgg ccaccccttc cggagtagag acgtgaacga tatggagccg tgcgcctgtg
     1201 cgccgggcga tctcgaccag ccgctgaatg gcggtcacct ccgcctcggc cgggcggacc
     1261 cgggcgtggc tgcgcggatc gctccagtca accgctgcgc acgcgctctc cggccagcga
     1321 gtgtccctgt ccgctgccgt ctccccctgc cgcgcccgct gcaggatggc gtcgtcctcg
     1381 cagtggacga ggacacggcc gccgaggctg tgcagccggc cgatgcgctc ctccagctcc
     1441 gtgtaggagg aatagagccc ggcctcgcgg taggtggtat agaacttgaa ggtgataaag
     1501 cccgcgccga tggccgcctc gatctcccgc cagccggcgt cgtcgaatcg ggtcggtgtc
     1561 aggtgccagg tgtaatcgca aaagctctgg ccagccgcct ttttttgagc ccgcgcgatg
     1621 gcagtgccca gcgattcatc gctcccttgt gtgatgaagg agcagaggga ggtgatgccg
     1681 ttctgcacgg cgatttggga tccgctgtgc caggtgtcgg cgaggtatct cccgccgatg
     1741 acgtcatcga gatgggtgtg gaggtcgata aagccgggga agaccaggct gcttgcggca
     1801 tcgaccgtca ggtcggccgg cggcagtgtg ctgcgagggg caatggcggc gatccgctcc
     1861 ccatcgatga gaatgtccgc ttgtttgagg cgatcgtcaa gaaccagagt gccgttttga
     1921 atggagattt tcatcggttt tgccgggccg gtttattgat gtccatgatg atgtaatata
     1981 gctttttacg gggaggagcg caagagcttt gtccagcatg ctgccccttt ctgcgctttt
     2041 ccggacagct ggcgttagcc tgacttattc ataaaatgtt ttcaaccact cttgtccaaa
     2101 ataggaaata aaatcatata gcaaaaaaac aagtctccgt agtttttttt tatcaggaaa
     2161 gaaaaacaaa caacggagga tccctccatt atgaaaatat atcaaataat tcttgacttt
     2221 tataacattt ttctgtaata atattacaac taaaataata agatgccctt aattctgcaa
     2281 ataactgact tattattata tttataataa atttgacgcg tagttcaaca acccagcagg
     2341 aggtgaactt ttttcaaagg aggtgcacgc aaggttgtat cagctccatg ggggtgtctg
     2401 gcgtgaatgc gtcggacctg taaccggcag atatccaaat tcaattcatt gacccattgt
     2461 acaattaaca cctgaaggag gccagatgaa caagtcgttt ctgtttttct tgctgatctg
     2521 tctcgtgcct ttcatcgtga cgggacaaaa catgctggcg aatgccggct ttgaggacgg
     2581 cgatgcagat gccaacggca ttccggatgg ctggatcggt tatgcacaaa ccggtgcaag
     2641 catgcagctg tataatgaag ctgccacagc ccactcggga cagtactggg caaaatgtac
     2701 aagcaccaac ggggggtatt atctcctcta tcagaatacc tttcctgcgg ctgaaggaga
     2761 tgtgtgggaa ttcagctcct tcatcaagga catttcccct gcttaccccg gggccgtctt
     2821 tgctgcgctg aaaatttcgg ccaagaacgc cgccggcggc accttcaagg cctgggagnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc caacggcatt ccggacggct
     3001 ggattggcta cgcccagact ggcgccagca tgcagctcat gaatgatgcc gccaccgcct
     3061 tcagcggctc ctggtgggcg aaatgcacga gcacaaacgg cggctattat ctcctctatc
     3121 agcaaacctt cccggccacc aagggcgagg tgtggaaatt cagctctttc atcaaggacc
     3181 tttcgccggc ctatccgggt gcggactatg tcgcgctcaa aatatcggcc aagaacgcca
     3241 acggcggcac cttccaggcc tatgaagtat tccagaccgg gctgaccagc tattggaaag
     3301 attattctaa cacccagacc atgcccgaag gcacacattt tatccaggcg gtcatcgtcg
     3361 tccacggcgc tgacggcgca cccgaggcca cctatggctt tgacgacgtg aggctggagc
     3421 tggtccacga gatgacgaac aacccgggat tcgaggatgg cgattcgaat ggcgatcaga
     3481 tcccggacgg ctggctcggc ggcggcacag ccggcaagac ccatatggaa acgatcaagg
     3541 attccacagc tcacagcggg cagttctgga ccaaggtcaa tgtcgacagc ggtgacggct
     3601 attatgtgct ctaccaggcc ggatttcccg ccaatcccgg cgagatctgg agtctgagat
     3661 cttttatcaa aaacatctca ccggattctg ccggcgattt tgctttcctc aagatttctg
     3721 caaaaaatgc caatggttcg aatgtgaagg tttgcgaagt tcgccaggcc ggcgtctcgc
     3781 ggcagtggaa ggactatcac aatacccaga ccatgcccct gggcacgacg ctgattcagc
     3841 ccgtgctggt ggtcaagaaa tccgccatag acagcgccgc tttctcgctc gttgcttcct
     3901 atgcatttga cgatgtgcgg gtggcgaaac tgggcgttgc cgacatcatg gccccgacgg
     3961 agcccggtgc ggtctcggtt gaaaacggcg ccaagccgaa caccaactcg gttacctgga
     4021 tggatgtggc tggtgaaacg ggcgagacct atgccctgta tgccagccag agcccgatca
     4081 ccaaggtgac cgatgcgggc gttgaagttc tggccactgc gattgccaaa ggcacgcaaa
     4141 aatatgagca ctccttgcgc aatccctaca ccgactcgct
//
LOCUS       DAOPWU010000312         4179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21462_length_4179_cov_4.258002, whole genome shotgun sequence.
ACCESSION   DAOPWU010000312 DAOPWU010000000
VERSION     DAOPWU010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21462_length_4179_cov_4.258002"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKM23_12790"
     CDS             complement(<1..604)
                     /locus_tag="PKM23_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92393.1"
                     /translation="MKPAILILGAGIYQAPLIRKARERGCEALVASIPGDFPGIALAD
                     QFYAVDIREVGNILALAQASHAAAILTTGADAGLPAIGAVVDTLGLRGPSRKIAETVS
                     SKSAFRAFLQAHGHPHPPFVCCPDGTEAWRFYSSVKRKCVFKPDDASGSRGVTILEPG
                     LPAERVAAAYEHARLHARNGLVCAEAFITGRETGGDAFFFD"
     gene            complement(601..1785)
                     /locus_tag="PKM23_12795"
     CDS             complement(601..1785)
                     /locus_tag="PKM23_12795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNY92394.1"
                     /translation="MIRAVLDRIRAFLQRTESIALLAFISTYLIGFIYPVFIHAERMY
                     FLKYFPPMNRLGADLSYTLEAATQAGRTPPYSPLAKVIFQLMNVVPWPLVYHLFTLLV
                     VGMVIVACYWIIPRLARYSSNVVITLIFLAILGSYGFHFELERGQWNMVVMVLVFASL
                     LAFRRGHANWSILLFTLAAQLKLYPFIFILIFLVRPLLSRKNLIHMAKLLGLNLVLFF
                     ILGAGALMRYSNMVVRYANSPEIWRGNHSIAAFSELVNTYFFSAPWLFPLLALVYLIL
                     LALVLAVTWRKGLAFDNKYLFFILVIGSLIIPSVSADYTLPALALALLYFLLDTRPEV
                     RWRKGLLLLALSAAVFLTHYSYVHMSGVFQFLRTNKLPLIYVIALLVAILSVVDNRRI
                     PS"
     gene            complement(1951..2859)
                     /locus_tag="PKM23_12800"
     CDS             complement(1951..2859)
                     /locus_tag="PKM23_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNY92395.1"
                     /translation="MSHQDASVKSILFALLANLGIAITKTVAAVLTGSGAMLAEAVHS
                     YADCGNQGLLFWGLKAAKRKRDPEHPLGYGKEIYFWSFIVALILFSMGGLFSIYEGVH
                     KLNSHEGLKSPMIAIAVLSVSILFEAASLTGCIRQINKLRHKVSLWIWAKKSRQSELV
                     VVLGEDTAALAGLGFALLAIILAVVTGNPVFDAIGSIGIGVLLIIISFSLGAKVKSLL
                     IGQSTDEETHARIEGMLSAMPEIERILNLITLQLGPQIMVAVKARMKPVDTVDELIKN
                     INRCEVVLRKANADIQWVFFEPDFRE"
     gene            complement(2959..3843)
                     /locus_tag="PKM23_12805"
     CDS             complement(2959..3843)
                     /locus_tag="PKM23_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNY92396.1"
                     /translation="MDHHHSHAQDSNASIRAAFFLNLIFSLIEVAGGFLTNSLAILSD
                     ALHDLGDSFSIGFSWFMESRARRPGHGSYSYGFRRLSLLAALVNAIVLLVGSVFILSR
                     AIPRLFAPEPAHPGGMLALAILGILVNGAAVLRMRNGKSLNEQMIVWHLLEDVLGWVA
                     VLVVSVALLISDLHILDPLLSMLITCYVLYNVVRNLHKTLAIFLQAAPPELDLAALDH
                     ALGAIEGVLSTHHTHAWSLDGEHNVLTTHLVVPATASRAHLIRVKKAALRLVEHADLE
                     HTTIEIEFEDENCRMRPD"
     gene            complement(3932..>4179)
                     /locus_tag="PKM23_12810"
     CDS             complement(3932..>4179)
                     /locus_tag="PKM23_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HNY92397.1"
                     /translation="ALTETGCNALTEVNWYTDRILKPLRRHPDLCRIAWILVWRNAYP
                     GHFYAPYPGHPSSQDFINFKNDELILFEDEMEPMYRL"
BASE COUNT          994 a         1140 c         1289 g          756 t
ORIGIN      
        1 catcgaaaaa aaaggcatcc ccgcccgttt ctctcccggt tatgaaggct tccgcacaga
       61 ccaggccgtt cctggcatgc agccgagcat gttcataagc cgccgcaacc ctctccgccg
      121 gcagccccgg ttcgaggatg gtgaccccgc gcgaacccga ggcatcatca ggcttgaaaa
      181 cgcattttcg tttcactgag gaataaaaac gccatgcctc tgtaccatcc gggcagcaga
      241 cgaaaggtgg gtggggatga ccatgggcct gcaaaaaggc gcgaaatgcg ctcttggagg
      301 agaccgtttc cgcgatcttg cgcgagggcc cgcgcagccc gagggtatcg acgactgcac
      361 cgatcgccgg cagaccggca tcggctccgg ttgtgaggat cgccgcggca tggctggcct
      421 gcgccagggc gaggatgttc cccacctccc ggatgtcgac tgcgtaaaac tgatcggcga
      481 gagcgatgcc tggaaaatcg ccgggaatgc ttgcgaccag ggcctcgcaa cccctctccc
      541 gggccttgcg gatcaacggc gcctggtaga tacctgcgcc caatataaga atcgcgggct
      601 tcaagagggg atgcgccggt tatcgaccac cgacaggatg gcgaccagga gggcgatgac
      661 gtaaatcagc ggcagcttgt tcgtccgcaa gaactgaaac accccggaca tgtggacata
      721 gctgtaatgg gttaaaaaga ccgctgcact gagggcaagc agcaagagcc ccttccgcca
      781 tcgcacctca ggacgggtgt cgagaagaaa gtagagcagg gccagcgcaa gcgccggcag
      841 ggtgtaatcg gcgctgacgc tcgggatgat cagggaaccg atgaccagaa taaaaaacag
      901 gtatttattg tcaaaggcca gtcccttgcg ccaggttacg gccaggacca gcgccagcaa
      961 aatcagataa acgagcgcca ataaaggaaa gagccatggg gcggagaaga aataggtgtt
     1021 gaccagctct gaaaaggcag cgatggaatg attcccgcgc cagatctccg gactgttggc
     1081 atagcgtacg accatattgg aataccgcat cagggcgcca gcgccgagga tgaaaaagag
     1141 caccagattg agtcccaaca gcttggccat gtgaatcagg tttttgcggg agaggagcgg
     1201 ccgaaccagg aaaatgagga taaaaatgaa tggatataat ttgagctgtg ccgccagggt
     1261 gaagaggaga atggaccagt tcgcatgccc cctcctgaaa gccagcaggg aggcgaaaac
     1321 caggaccatc accaccatgt tccattgccc gcgctcgagt tcaaaatgaa aaccatacga
     1381 tcccaaaatg gccagaaaga taagcgttat cacaacattg ctgctgtagc gggcaagtcg
     1441 ggggatgatc cagtaacagg cgacgattac catgcccacg accagcagcg tgaagaggtg
     1501 atagaccaag ggccagggaa cgacattcat taattgaaaa ataaccttgg caagagggga
     1561 ataggggggc gttcgccccg cctgagtggc cgcctcgagg gtgtaggaga ggtcagctcc
     1621 cagacggttc atggggggga aatatttgag gaagtacatg cgctcggcat ggatgaagac
     1681 cggatagata aagccgatga gataggtcga tataaaagcg agcagcgcga tgctttcggt
     1741 tctttgcaga aacgcccgga tcctgtccag aacggcacgg atcatcctct cctccctccc
     1801 ttggtgcgca ctctccgcga tgagacagct cgcaggttcc gttaagattc tccacctgga
     1861 ttcccggcca gagccgggcg acaggagaat gacatgtctg caatccggaa aaagagccga
     1921 cagatccggc catcccgcag cgccggccgg ctattccctg aaatcaggct caaaaaatac
     1981 ccactgaata tccgcatttg cctttctcag caccacttcg caccggttga tatttttaat
     2041 cagctcatcc acggtatcga ccggcttcat cctggctttc accgccacca tgatctgcgg
     2101 gcccagctgc agggtgatca gattgaggat gcgctcgatt tccggcatgg cggagagcat
     2161 cccttcgatg cgggcgtggg tttcctcatc ggtgctctgc ccgatcagca ggctcttgac
     2221 cttcgctccg agcgaaaagg agatgataat gagcaaaacg ccgatgccga tgctgccgat
     2281 ggcgtcgaag acgggattgc cggtgacgac agcaagaatg atcgcgagca gggcgaagcc
     2341 caggcccgcc agggcggcgg tgtcttcgcc caggaccacc accagctcgc tctgccggct
     2401 ctttttagcc caaatccaca ggctgacctt gtgccgcagc ttgttgatct gtctgatgca
     2461 gccggtgagc gaggcggcct cgaagaggat gctcacggaa aggaccgcga tggcgatcat
     2521 cggactcttc agtccttcgt gagagttgag cttgtgcact ccctcataga tcgaaaagag
     2581 gccgcccatg ctgaagagaa tgagggcgac gatgaaggac cagaagtaga tctccttgcc
     2641 gtagccgagg ggatgttccg ggtcgcgctt tcgcttcgcc gccttgaggc cccagaagag
     2701 cagcccctgg ttgccgcaat cggcgtagga gtggaccgct tcggcaagca tggcccccga
     2761 gccggtgagc acggcggcga cggttttggt gatggcaatg ccgaggttgg ccagcaaggc
     2821 aaaaaggatt gatttgaccg aggcgtcttg atgtgacatt tgggatttat ccgcattaaa
     2881 gtgggcaggc tgattgcggc ggcaatatag ccaacccggc cgtgaaattc aagtatattc
     2941 tcgcagcacc cggtcgacct aatccggccg catccggcag ttttcgtcct cgaattcaat
     3001 ctcgatggtg gtatgttcga ggtctgcgtg ctcgaccagc cttagtgcgg ctttcttgac
     3061 gcgaatgagg tgcgcacggg aggccgtggc aggaacaacg aggtgggtcg tgagcacatt
     3121 atgctccccg tccagcgacc aggcatgggt atgatgggtc gagagcaccc cctcgatggc
     3181 gccgagcgca tgatcgagtg ccgccagatc gagttcgggg ggcgccgcct gcagaaagat
     3241 cgccagggtt ttgtggaggt tgcgtaccac attgtagagc acataacagg tgatgagcat
     3301 ggagagcagt gggtcgagga tatggaggtc ggagatgagc agcgcaacgc tgacgaccag
     3361 gacggcgacc cagccaagca catcctcgag caggtgccag acgatcatct gttcattgag
     3421 ggacttgcca ttgcgcatgc ggagcacggc tgcgccattg acgaggattc ccagaatggc
     3481 aagggcgagc ataccgccgg gatgggcggg ctccggcgcg aacaggcggg ggatagcgcg
     3541 ggagagaatg aaaacagagc ccacgagcag tacgatggcg ttcaccagtg cggccagcag
     3601 cgacaaacgc ctgaatccat aggaatagga gccatggccg ggcctgcgcg cacggctctc
     3661 catgaaccat gaaaagccga tcgagaagga gtcacccaga tcatgcaggg catccgaaag
     3721 gatcgccagg ctgttggtga ggaatccccc ggccacctcg atcagggaaa aaatcaggtt
     3781 aaggaaaaac gccgctctta tactggcgtt agagtcctgc gcgtgtgaat gatggtggtc
     3841 catgctcgtt cctgaatgga gatccgcctg cgggccggga aactcgccca attcatcccc
     3901 ggatcgactc gatccccctc ccctgcttcc tctacaacct gtacatcggc tccatttcat
     3961 cctcgaagag aatgagctcg tcgtttttaa agttgatgaa atcctgactg gagggatggc
     4021 cgggataggg cgcatagaaa tgaccgggat aggcattgcg ccagaccagg atccaggcaa
     4081 tccggcacaa atccggatgg cggcggagcg gcttgaggat gcggtcggtg taccagttga
     4141 cctcggtgag ggcgttgcag ccggtctcgg tcagggccg
//
LOCUS       DAOPWU010000313         4159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21585_length_4159_cov_4.564084, whole genome shotgun sequence.
ACCESSION   DAOPWU010000313 DAOPWU010000000
VERSION     DAOPWU010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21585_length_4159_cov_4.564084"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..3284
                     /locus_tag="PKM23_12815"
     CDS             294..3284
                     /locus_tag="PKM23_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY92398.1"
                     /translation="MKCLNKLVHFISILLLIFFIASDLHAAPGGKISGRVTDRATRQP
                     LPGANVMIKDTRLGAVTDPDGYYFIINVPPGTYRLQASLMGYATVVQEDLDVNLNQTT
                     NADFTLPQQAIEGETVVIKASRPAIQRDISSSQVIVSEEAIQSRPLENLEQILATEAG
                     INLTAGTDGTGLVVRGGGLEETDIVIDGLSTRNERNQQPLTTISLTAIKEIEVLTGGF
                     NAEYGDIRSGMISVITKEGSLDRYSVNADLRVSPPARKHFGASPFSVDGPFWRTYAGK
                     DAFTGVTQKMVDDGQYPFTFVGWNEVARQFAADPDPGNDVTPQEALEIWKWQHRIRKY
                     ADKPDYIGDVSISGPIPKTGIAFLASQRYEDLQLIYPFSRNNSIGSTTLLKLTTNLTP
                     KMKLSFNNQYMMIKGVAGSIYEGSTGVITGSREGNQYAQNAFFWRYMWNDASFNPVDI
                     QQYRGGLSLTHVLSPQTYYDARIEYTNYRIVQEPIGLRDTTGVKQIGGKWYDEAPWGY
                     VGSALGSITEKYDILGDFLMSGGGRGQDHSRYWGVRLSADMVSQIDRHNEIKTGIELD
                     YTTFKERREINHGQTTQPYTEAPENWWYYDQSPIKLGAYIQDKLEYMGMIANIGVRMD
                     YMQPGTDPYNLDPQFIFSQLPYNMLAFQENGNSFSNFATGDKSYKLYLSPRLGISHPV
                     TSSSKIFFNYGHFYQPPVTDYLYTVRPRTSGAELPNLGAEWPRTVAYEIGIEKSFAQS
                     LLVRFMGYYKDVTNQLSRQDIVAIDEASTVSTWANNSYADIRGLELRLEKRLGQWWHG
                     YVTAEYMVRSTGFTGLRYIYEDRQLAREQRENTNQQRGYPVPSLTADLTFKTPIDFGP
                     LFMGIRPLGNWQLNLLQGWSDGGKELLNPDALLSEQRWVESIDWWNTDLMLEKRFDFN
                     RKRVGFFMQVRNVFDYKGFVSPFYWNKYVDSLHLPWEKGDQKGNDKLGEGPSSDKPYI
                     DVGWNDWSHYINPRDIFFGVRIQF"
     gene            3316..>4159
                     /locus_tag="PKM23_12820"
     CDS             3316..>4159
                     /locus_tag="PKM23_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92399.1"
                     /translation="MKLNIEKIRFCWRIGLFMLLLLPVAYTNGFGQTRTHDLGDFKLR
                     IEADETINTNDVKPTGEWPQDHFRYAQIVFHNSGHVVGKWIDSTGVDHIKEDFFWPVS
                     FNRDAPYGLYEYRRSKPPEVWVYANGELQLSSRRFNGTVDPTLPADIMIESHYKSYPG
                     FDVVSRSYSFTNPEHDDYVIFRTRYLCTFDWDQDPEPDTNTDQVMENVYFFVGYSFQT
                     AEGTYITYSRWYEEGKDDWATFETYTPVLAAGGRDLNVSYGWDGDHPDLNEFEAGGQE
                     FDDTG"
BASE COUNT          944 a         1201 c         1146 g          868 t
ORIGIN      
        1 gtcagacctc caatgcggga tatagcaagc cagtcgagat ctggaactac gctctgaatg
       61 gcagcacgca gttcatcttt gtggaccgca acaatgacgg ccactgggtt ctcgtccatt
      121 ccaatcatcc agatgaattt tcaaaccatg agtgggagaa gcagattcaa tagccaatga
      181 atggaggtga tgacattcct gcctgagtgt tgttagtgag agtggggcaa tcagccgtgc
      241 cccccgccag tgggcggagg ggagcgatct attcaaaaga aggagtcaca gacatgaaat
      301 gccttaacaa gttggtccat ttcatttcca tccttctcct gatctttttc atcgcgagcg
      361 atttgcacgc cgccccggga ggcaagatca gtggccgggt gacggaccgg gcaaccaggc
      421 agccgttgcc tggcgccaac gtgatgataa aagatacacg tttgggagcg gtgacggatc
      481 cggacgggta ctacttcatc atcaatgtgc cgcccggaac ctaccggttg caagcttccc
      541 tgatgggata tgccacagtg gttcaggagg atctcgacgt caatctcaac cagacgacga
      601 acgccgattt caccttgccg caacaggcca ttgagggtga gacggtggtc atcaaggcca
      661 gccggccggc tatccagcgc gacatctctt cgagccaggt catcgtctcc gaggaggcga
      721 ttcagagccg tcccctcgag aatctggagc agatccttgc aaccgaagcc ggcatcaatc
      781 tgaccgccgg cacggacggc accggtctgg tcgtcagggg tggcggactg gaggagaccg
      841 acatcgtcat cgacggacta tcgacgcgca acgagcgcaa tcagcagccc ctgaccacca
      901 tcagcctgac ggcgatcaag gagatcgagg tgttgacagg cggtttcaat gcggaatacg
      961 gcgacatccg ctccggcatg atcagcgtta tcactaagga gggcagcctc gaccgctatt
     1021 ctgtgaatgc cgatctgcgc gtcagtcccc ccgcccgcaa gcatttcggc gccagtccct
     1081 tcagcgtcga tgggcctttc tggcgcacct atgccgggaa ggacgccttc accggtgtga
     1141 cccagaagat ggtcgatgac ggccagtatc ccttcacctt tgtcggctgg aacgaagtgg
     1201 cgcggcagtt cgctgcggat ccggatcccg gtaatgatgt gacgcctcaa gaggcgctgg
     1261 agatctggaa gtggcagcat cgcatccgca agtacgccga caaacccgat tacatcggtg
     1321 atgtgtcgat cagcgggccg atcccgaaaa caggcatcgc ttttctggcc tcccagcgct
     1381 atgaggatct gcagctgatc tatcctttca gccgaaacaa ctccatcggg agcaccaccc
     1441 tgctgaaact gaccacgaat ctcaccccga agatgaaact ctctttcaac aatcagtaca
     1501 tgatgatcaa gggggttgcc ggaagcatct atgagggatc gaccggcgtg atcaccggtt
     1561 cccgcgaagg caaccagtat gcgcagaatg cctttttctg gcgctacatg tggaatgacg
     1621 cctctttcaa tcccgtcgat atccagcagt accgcggcgg attgagcctt acccatgttc
     1681 tgagccccca gacctattac gatgcgcgca tcgagtatac caattaccgc atcgttcagg
     1741 agccgatcgg cctgcgcgac accacgggtg tcaagcagat tggcggcaag tggtacgatg
     1801 aggcgccgtg gggctatgtg ggcagcgccc tcggttcaat cacagaaaag tacgacatcc
     1861 tgggagattt cctcatgtcg ggcggcggcc gcggtcagga ccactcccgt tactggggag
     1921 tgcgtctcag tgcggacatg gtttcgcaga tcgaccgcca caatgagatc aagacaggca
     1981 tcgagctgga ttacaccacc ttcaaggaga ggcgtgaaat caatcatggc cagaccacgc
     2041 agccctatac ggaagcgccc gaaaactggt ggtactacga tcaatccccg atcaagctcg
     2101 gcgcctacat ccaggacaag ctggaatata tgggcatgat cgccaatatc ggcgtgcgca
     2161 tggattacat gcagcccggc accgatccct acaatctcga tccccagttc atcttcagcc
     2221 agctgcccta caacatgctg gctttccagg agaacggcaa cagctttagc aattttgcta
     2281 ccggggacaa gtcctacaag ctttacctca gccccaggct cggcatctcc cacccggtga
     2341 cctcgtcgag caagatcttt ttcaattacg gccattttta ccagccgccg gtgaccgatt
     2401 atctctatac cgtccggccg cgcaccagcg gcgcagaact ccccaatctg ggcgccgaat
     2461 ggccgcgtac ggtcgcctac gagatcggca tcgagaagag tttcgcccag tccctgctgg
     2521 tgcgcttcat gggctactac aaggatgtca ccaaccagct cagccgtcag gacatcgtgg
     2581 ccatcgacga agccagcacg gtctcaacct gggccaacaa cagctatgcc gatatccgcg
     2641 gactggagct ccggctcgaa aagcggctgg gacagtggtg gcacggttat gtcacggctg
     2701 aatacatggt gcgcagcacg ggtttcaccg gcctgcgcta catctatgag gatcggcagc
     2761 tggcccggga gcagcgcgag aataccaatc agcagcgcgg ctatccggtg ccgtcactta
     2821 cagccgactt gaccttcaag acaccgatcg atttcggtcc gctcttcatg ggcatccgtc
     2881 cgctcggcaa ctggcagttg aacctcctcc agggctggtc ggatggcggc aaggagctgc
     2941 tcaatcccga tgccctgctg agcgaacagc gctgggtgga atcaatcgac tggtggaaca
     3001 ccgacctgat gctggagaag cgtttcgatt tcaatcgcaa acgcgtcggt ttcttcatgc
     3061 aggtgcgtaa cgtgttcgat tacaagggat ttgtcagccc cttctactgg aataaatatg
     3121 tggattccct ccacttgccc tgggaaaagg gtgatcagaa agggaacgac aaactgggcg
     3181 agggtcccag ctccgacaag ccctacatcg atgtgggctg gaatgactgg tcgcattaca
     3241 taaatcccag ggatatcttc ttcggcgtca ggattcagtt ctagccgcct gatcctaatt
     3301 aggaggtcaa agctcatgaa gcttaacata gaaaagatcc ggttctgttg gcgcataggg
     3361 ctgttcatgc tcttgctgct gccggtggcc tacaccaacg gattcggcca gacccgtacg
     3421 catgacttgg gcgatttcaa attgcgcatc gaagcggacg agaccatcaa taccaatgat
     3481 gtcaaaccaa caggcgagtg gcctcaggat catttccgtt atgcccagat cgtgtttcat
     3541 aacagcggtc atgtcgtcgg caaatggatt gattcgacgg gcgtcgatca tatcaaggag
     3601 gactttttct ggccagtctc tttcaaccgg gatgccccct atggtcttta cgaataccgg
     3661 cgcagcaagc cacccgaagt ctgggtttac gccaatggcg agttgcagct ctcctcgcgg
     3721 cggtttaacg gcactgtgga tcccactctg cccgcggata tcatgatcga gtcgcactac
     3781 aagtcctacc ccggtttcga tgtcgtctcg cgctcctact cgttcactaa tcccgaacat
     3841 gacgattatg tcatcttccg cacccgctac ctctgcacct tcgactggga tcaggatcct
     3901 gaacccgata ccaacactga ccaggtgatg gagaatgtct actttttcgt cggctacagt
     3961 tttcagaccg ccgaaggcac ctacataacc tattcccgat ggtatgagga aggcaaggat
     4021 gattgggcca ccttcgaaac ctatacgccg gttctggcgg cgggcggcag ggatctgaat
     4081 gtctcttacg gctgggacgg cgatcatccg gatctcaatg agtttgaagc gggcggtcag
     4141 gagttcgacg ataccggcg
//
LOCUS       DAOPWU010000314         4145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21683_length_4145_cov_4.711980, whole genome shotgun sequence.
ACCESSION   DAOPWU010000314 DAOPWU010000000
VERSION     DAOPWU010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21683_length_4145_cov_4.711980"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1023)
                     /locus_tag="PKM23_12825"
     CDS             complement(91..1023)
                     /locus_tag="PKM23_12825"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNY92400.1"
                     /translation="MPLQQKHLLGLEGVSAADITAILETARTFREILERPIPKVPTLR
                     GQTVVNLFFEPSTRTRISFELAEKRLSADSVNFTTSSSSVKKGETLKDTIRNIAAMKI
                     DMVVVRHQSAGVPLFLTRCVDAAIINAGDGAHEHPTQALLDMFTLREKFGRLEGLKVA
                     IIGDISHSRVAGSNIRGLVTMGAEVLLCGPRTLMPREVERFGVRVTHDIEEAIAWADV
                     LNVLRIQLERQEAGLFPSLREYHAEFGVTLPRLERAKKEITIMHPGPINRGVELDSDV
                     ADADYSVILDQVTNGVAVRMAVLYLLSGNEESVG"
     gene            complement(1024..1581)
                     /gene="pyrR"
                     /locus_tag="PKM23_12830"
     CDS             complement(1024..1581)
                     /gene="pyrR"
                     /locus_tag="PKM23_12830"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNY92401.1"
                     /translation="MDVKVKAVVIDKEGLKRTISRLAFEIIERNHGVDKIAIVGVRTR
                     GAFVAERIVKRIEELEGKRVPYGVLDITLYRDDFRKRLRQPVVQVTDIPFEIDDIPVV
                     IVDDVLFTGRTSRAAMDALMDFGRPAAIQLAVLVDRGHRELPIHADYVGKSIPTSIGE
                     EVQVRLKESDGEDCVLLVESPKEAQ"
     gene            complement(1703..2944)
                     /gene="lysA"
                     /locus_tag="PKM23_12835"
     CDS             complement(1703..2944)
                     /gene="lysA"
                     /locus_tag="PKM23_12835"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNY92402.1"
                     /translation="MIPYSYRDNRLFCEETDLAEVARQYGTPAYVYSKAGITGNYARI
                     DAAFAAAPHLVCYALKANANPALLKMMAELGAGADVVSGGELAQALQAGFPAQRIVFA
                     SVGKTDAEIRFAIRSGILALNVESREELEVTARLAAEMGMTAPIALRINPDIDIEGHP
                     YLTTGKSANKFGIALEEARAAYLWAAEQNSLKIVGVHCHIGSMIKKSDPYHRMAETLA
                     AFVADLGKAGIHFEHVDLGGGLGVDYTKVLGEQGSPWFMEPEEMAAQVIPVLQPLGCE
                     ILLEPGRSIVGPNGVLLSQVLYRKETKGKKFLVVDAAMSDLIRPSLYGAYHAILPLQA
                     GGAELETVDVVGAICESGDFLAKDRELERLQRGDWLAVMTAGAYGYTLTSNYNARPRP
                     VEVLIDGATHSVIRDREPSLF"
     gene            complement(3008..3973)
                     /gene="trpS"
                     /locus_tag="PKM23_12840"
     CDS             complement(3008..3973)
                     /gene="trpS"
                     /locus_tag="PKM23_12840"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNY92403.1"
                     /translation="MRVLSGIQPSGNLHLGNYFAMMKRMIDYQEHHTLFCFVVNLHAL
                     TSVFEAQKLRENTFAACCDFLALGIDPEKSYFYVQSDVPEVTELTWLLSNVTPMGLLE
                     RGHSYKDKLAKGIPANHGLFAYPVLMAADILLYGADIVPVGKDQKQHLEMTRDIAIRF
                     NQTYGEVFVVPEPDIPDSVAMVPGLDGQKMSKSYGNTIDIFSPENVMKKRVMSIVTDA
                     TPVEAPKNPDTCNLFAIYSLFLDEAGRKELRARYLAPGLKYGEVKKELAEVIWNYFAP
                     YRQRRDELMKDPEGVHRIMQRGAEKARAVAAHYLEAARHATGIDY"
BASE COUNT          760 a         1245 c         1307 g          833 t
ORIGIN      
        1 cctggcgctt cccggaggcc agatcgagca cctcgccgcc ctgaaggagg attttggtgg
       61 ctttttgcag gttttccatc gattttcctt tcatcccacc gactcttcat taccgctgag
      121 cagatagagc actgccatgc gcaccgccac gccgttggtc acctggtcga ggatgaccga
      181 atagtcggcg tcggccacgt cgctgtcgag ctcgaccccg cggttgatcg gaccggggtg
      241 catgatggtg atctccttct tcgcccgttc cagcctcggc agggtcaccc cgaattcggc
      301 atgatactcg cgcagcgagg ggaacaatcc cgcctcctgg cgttcgagct ggatgcgcag
      361 cacattgagc acatcggccc aggcgatcgc ctcctcgatg tcgtgggtga cccggacgcc
      421 aaagcgctcc acctcgcgcg gcatcagggt cctggggccg caaagcagca cctccgcccc
      481 catggtcacc agaccgcgga tgttggagcc ggcgacgcgg ctgtgcgaga tgtcgccgat
      541 gatcgccacc ttgagcccct ccagccggcc gaatttttcc cgcagggtga acatgtcgag
      601 cagggcctgg gtggggtgct catgggcgcc gtcgccggcg ttgatgatcg cggcatcgac
      661 gcagcgggtg agaaagagag gcactcccgc ggactggtgg cgcaccacta ccatgtcgat
      721 cttcatcgcg gcgatgttgc ggatggtatc cttgagggtc tccccttttt tgaccgaact
      781 ggaggaggtg gtaaaattga ccgaatcggc ggagaggcgc ttctccgcga gctcgaagga
      841 gatgcgcgtg cgcgtcgagg gctcgaaaaa gagattgacc accgtctgcc cgcgcagggt
      901 cgggaccttg gggatggggc gttcgaggat ctcgcggaag gtccgcgccg tctccaggat
      961 ggcggttata tccgcggcgc tgaccccctc cagtccaagc aggtgttttt gttgcagcgg
     1021 catctattgg gcctccttgg gggattcaac cagcagcacg caatcctcgc catcgctctc
     1081 cttcagccgc acctgcactt cctcgccgat ggaggtgggg atgctcttgc cgacatagtc
     1141 ggcatggatc ggcagctcgc ggtggccgcg gtcaaccagc acggcgagct ggatcgccgc
     1201 cggccggccg aaatccatca gggcgtccat cgccgcccgt gaggtccgcc ctgtaaagag
     1261 cacatcgtcg acgatgacca ccgggatatc gtcgatttcg aaggggatgt cggtgacctg
     1321 gaccaccggc tggcgcagcc gcttgcggaa atcgtcgcga taaagggtga tgtcgagcac
     1381 cccgtatggg acccgttttc cctccagctc ctcgatacgc ttgacgatgc gctcggcgac
     1441 aaaagccccg cgcgtgcgca ccccgacgat ggcgattttg tccacgccgt ggttgcgctc
     1501 gatgatctca aaggcgaggc gcgagatggt ccgtttcagc ccctccttgt cgatgaccac
     1561 tgccttgact tttacgtcca tttttcaccc gtattctggt tcaaaccata agaattactc
     1621 tcaccaaacc tccaagacac aaagaaataa ctagttatgc ttctctttgt gactttgtgc
     1681 ctccgtgaga ggttaacttt agtcagaaca gcgacggctc gcggtcgcgg atgaccgaat
     1741 gtgttgcgcc gtcgatgagc acctccaccg gccgcggccg cgcgttgtaa ttgctggtca
     1801 gggtatagcc gtaggccccg gcggtcatca ccgccagcca atccccgcgc tgaagccgct
     1861 ccagctcgcg atccttggcg agaaaatcgc ccgattcaca gatcgccccg accacatcga
     1921 cggtctccag ctcggccccg ccggcctgga ggggcaggat ggcgtggtag gcgccgtata
     1981 gcgaaggccg gatgaggtcg ctcatcgccg catcaaccac caggaatttc ttgcccttgg
     2041 tctccttgcg gtagagcacc tgcgagagca gcaccccgtt ggggccgacg atggagcggc
     2101 ctggttcaag caggatctcg cagcccaggg gctgaagcac cggaatcacc tgtgccgcca
     2161 tctcctccgg ctccatgaac cagggcgagc cctgctcacc cagcactttg gtataatcga
     2221 cgccgagtcc gccgccgagg tcgacgtgtt caaagtggat gccggccttg cccagatcgg
     2281 caacaaacgc ggccagggtc tcggccatgc ggtggtaagg atcgctcttc ttgatcatcg
     2341 agccgatgtg gcagtgcacc ccgacgattt tcagggagtt ctgctccgct gcccagaggt
     2401 aggcggcgcg cgcctcctcc agggcgatgc cgaacttgtt ggcgctcttg ccagtggtca
     2461 gatagggatg gccctcgatg tcgatatcgg gattgatgcg cagggcgatc ggggcggtca
     2521 tgcccatctc cgcggcgagg cgggcggtca cctccagctc ctcgcgcgac tcgacgttga
     2581 gggcgaggat gccggaacgg atggcgaaac ggatctcggc gtcggtcttg ccgacgctgg
     2641 cgaagacgat gcgctgcgcc gggaatcccg cttgcagggc ctgggcgagc tcgccgcccg
     2701 agaccacgtc ggctccggcc cccagctccg ccatcatctt gagcagggcg ggattggcgt
     2761 tggccttgag ggcgtagcag accaggtggg gggcggcggc gaaggccgcg tcgatgcggg
     2821 cgtaattgcc ggtgatgccc gccttgctgt agacataggc cggtgtgccg tactggcgag
     2881 ccacctcggc cagatcggtc tcctcacaga acaaacggtt gtcgcggtag gaatagggga
     2941 tcatggcagg cccttcctca ggttatctca aaaaaaagag cgatggatct cctccatcgc
     3001 tcacggttca ataatcgatg ccggtggcgt gccgggccgc ctcgagatag tgcgcagcca
     3061 cggcgcgggc tttttctgcg cctctttgca taatgcggtg aaccccctcc ggatctttca
     3121 tcagctcatc gcggcgctgg cggtaggggg cgaaatagtt ccagatcacc tcggccagct
     3181 cctttttcac ctcgccgtat ttgagtcccg gcgcgaggta gcgggcgcgc agctccttcc
     3241 ggccggcctc atcgagaaag agcgaataga tggcgaagag gttgcaggtg tccggattct
     3301 tgggcgcctc aaccggggtg gcgtctgtga cgatcgacat caccctcttt ttcatgacat
     3361 tctccggaga aaagatgtcg atggtgttgc cgtaggattt ggacatcttc tggccgtcga
     3421 ggccgggcac catcgccacc gaatcgggaa tgtccggttc gggcacgaca aagacctcgc
     3481 cgtaggtctg gttgaagcga atggcgatgt cgcgcgtcat ctcgaggtgc tgcttctggt
     3541 ccttgccgac cggaacgatg tccgctccgt agaggaggat gtcggcggcc atcagcaccg
     3601 ggtaggcgaa gaggccgtgg ttggccggga tgcccttggc cagcttgtct ttataggagt
     3661 ggccgcgctc gagcagtccc atgggggtga cgttggagag cagccaggtc agctcggtca
     3721 cctcaggcac atcggactgg acatagaaat aggatttttc cggatcgatg ccgagggcga
     3781 ggaaatcgca gcaggccgca aaggtatttt cgcgcagttt ttgcgcttcg aaaaccgagg
     3841 tcagggcgtg gagattgacg acgaaacaaa agagcgtatg atgctcctga tagtcgatca
     3901 tccgcttcat catggcgaaa tagttgccga gatggaggtt gccggagggt tgtatgccgg
     3961 agaggacgcg caaggtcgtt ttccttgatg ggttgaggac tttataattc tctaaaaata
     4021 cagttttttt tgcagccaat caagagaaaa gaggggcagg gtacccccga aatctgggtc
     4081 ccacctgacg cctggcacag cgtaccccaa tcccaccgta cgatttccag catgttctcg
     4141 gcctg
//
LOCUS       DAOPWU010000315         4132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21772_length_4132_cov_5.898455, whole genome shotgun sequence.
ACCESSION   DAOPWU010000315 DAOPWU010000000
VERSION     DAOPWU010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21772_length_4132_cov_5.898455"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            594..800
                     /locus_tag="PKM23_12845"
     CDS             594..800
                     /locus_tag="PKM23_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92404.1"
                     /translation="MDRTKTAKKHWDKPQLIVIGRGKPEESVLAGCKSHAQPHGTLAT
                     ATKTNCNKLDGSCGSCQNNGGGIS"
     gene            819..2837
                     /locus_tag="PKM23_12850"
     CDS             819..2837
                     /locus_tag="PKM23_12850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY92405.1"
                     /translation="MARPYTAYDPTRKTPRLPRDGSIDLTYRCNFQCRHCWVRVNGSD
                     GDRPQELSLDEIAAIANDARKMGCRAWTISGGEPMLRPDFPEILDLLTRAPFSYSINS
                     NGSLITPAIAKLMTRRGNKMIALYGADAAVQDAVTRQPGSFEATLRGMQYLKEAGAGF
                     TVQIIPMRSNYHQFEKMVALARQLSPSWRIGAAWLFLTADRLAGRNAIIKKERLPAET
                     VNIIDPAIPACGPPPSCARSVPGMNQPPEHQLFAACVNDTQQFHIDPWGGMSFCSTIK
                     DPAARFSLREHTFEKIWEVDLPAYAGRTLATAEYHENCGRCALKNYCHWCGAYAWLET
                     GRTSAPVRYLCEIARERRKFDEEWRSRNRRCFNLAGLELQVDSDLPFREDGFAHKFND
                     FYIQSAGEDALRIRHHYALPEPSLWAGGELVYDKTPWQIYRKGDLWIYLGIGRKKKHL
                     DINTVAVFNSAHTIADIYHHDDRMVRQARFCSLTALPSDHIMLAHPLADRQAMVMHSS
                     GLVLHGQGYLFAGQSGAGKSTVIKLLRDYGYVLCDDRVVVRKWPEGFRLHGTWSHGEI
                     PVTSNLQAPLKAILLLKKARRCKIEPILDPKEIMHALLIRVARPVQTAEWLKKVLDLL
                     GALAGEVPVYRLSFTRDARIVEAMETLTGPLQALKTAASIPPVETRHD"
     gene            2830..4023
                     /locus_tag="PKM23_12855"
     CDS             2830..4023
                     /locus_tag="PKM23_12855"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY92406.1"
                     /translation="MTSPAASAPAQGYLAEQPALRWNGARPLLTVFDIELTERCNNNC
                     IHCCINLPERDRSARKRELSTEAVEELLRQGAELGALTVRFSGGEPLLRDDFTELYLY
                     ARRLGLRVMLFTNARLITPELAGLFARIPPREPVEITVYGMSADSCAAVTRSRGAFAE
                     FRRGVDLLIRHRIPFFLKGTMLPETRSELEQLLSWSQSIPGMKELPSFVLFLELRHRR
                     DSEARNRQIEKLRLSPREAIEVLARDPDVYKADLHRLCRYVAGKPTDLLFTCNAGNQI
                     CIDAYGQIQYCLALRHPGTVVNSSIYPLEFALREFFPQLRQMRATNPDFINRCARCML
                     RGLCEQCPAKSWIEHGVLDQPVEYCCEAAHEQARYLGVLTGDEKGWEVADWGERLRRI
                     NVGEG"
BASE COUNT          844 a         1326 c         1158 g          804 t
ORIGIN      
        1 atttaaaata gtacttgaca tgacgatcat gaatgactat ctttcagtgt gcaataattg
       61 aacaacttgg cttttttagc gacacagttc aagttcaggg tattcgcaat cagcaccgtt
      121 gacattcagg catcctcgat ttctgcatct tttgcccgct tcgtacccgc aactttcagt
      181 gaattcattc agttgtactg ttcagatttt tgcatcacca attccgcaag cgccaggcct
      241 cttttccggc taccagctac cctgagccgt aactgaatcc gggcctgtcg ccggcggagt
      301 tgcacaggca ggggatataa agggcggcgg agcggtggcc aagctgggtt attatgagct
      361 gtacggcttt tatgtcacat cccacagaac cggtgtcccg caaagcggtc aggtcttatt
      421 caagtctgac cattctcttc tcttaactcc atatttatca attttaacag atctttccag
      481 ttagtggcat aattcttgaa cgtacacttc cggctatgga tccggctcgg ccgggcggcc
      541 gttatctcag gcagtgggaa atgagatcca ccaattcact ttaaggaggc agcatggaca
      601 gaactaaaac cgccaaaaaa cattgggaca aaccgcagct gatcgtgatc ggacgcggca
      661 agccggaaga atcggtgctg gcgggctgca agagccacgc ccagccccac ggcaccctgg
      721 cgacagcgac taaaaccaac tgcaacaaat tggatggatc ctgtggctcc tgccaaaaca
      781 atggcggcgg gatctcctga cccttgaccg gaaataaaat ggcccgcccc tacactgctt
      841 acgacccgac cagaaaaacg ccgcgattgc cccgggatgg ctccatcgac ctgacctatc
      901 gctgcaattt ccagtgccgt cactgctggg tgcgtgttaa cggctccgac ggcgacaggc
      961 cgcaggaatt atccctggat gaaatcgccg ccatcgccaa cgatgcgcgt aaaatgggct
     1021 gccgcgcctg gaccatctca ggcggcgagc ccatgctgcg ccccgatttc cccgagatcc
     1081 ttgatctgct cacgcgcgcg cccttttcct acagcatcaa cagcaacggc tcgctgatca
     1141 ctccggctat cgccaaactg atgacccgcc gcggcaacaa gatgatcgcc ctttacgggg
     1201 ccgacgccgc ggtccaggac gcagtaaccc gccagccggg atcgttcgag gcgaccctgc
     1261 gaggtatgca gtacctcaag gaagccggcg ccggattcac ggtgcagatc atccccatgc
     1321 ggtccaatta ccaccagttc gaaaaaatgg tcgcactggc caggcagctc agcccctcct
     1381 ggcgtatcgg cgcggcctgg cttttcctca ccgccgatcg gctggctggt cgcaacgcca
     1441 taatcaaaaa agagcgcctg cccgccgaaa ccgtcaacat catcgacccg gccattccgg
     1501 cctgcggtcc cccgccctcc tgcgcccgtt ctgtgcctgg catgaatcaa ccgcctgaac
     1561 accagctgtt cgcggcgtgc gtaaacgaca cccagcagtt tcacatcgac ccctggggcg
     1621 ggatgagctt ttgctccacc atcaaggacc cggcggcgcg tttcagtcta cgcgaacaca
     1681 cttttgaaaa gatctgggag gttgatctgc ccgcctacgc cgggcggacc ctcgccaccg
     1741 ccgagtatca cgaaaactgc ggccgctgcg ctctgaaaaa ctactgtcac tggtgcggtg
     1801 cctatgcctg gctggagacc ggcaggactt ccgccccggt tcgctatctt tgcgagatcg
     1861 cgcgcgaacg gcgtaaattc gacgaggaat ggcgcagccg caaccgccgc tgtttcaacc
     1921 tggcggggct ggagctgcag gtcgattccg atctcccctt ccgggaagat ggctttgcgc
     1981 acaaattcaa cgacttttac atccagtccg ccggcgagga tgccctgcgc atccgccacc
     2041 actatgccct gcccgagccg tcgctctggg cgggaggcga gctggtctac gacaagacgc
     2101 cctggcaaat ttaccgcaag ggcgacctgt ggatctatct cggcatcggc cgtaaaaaga
     2161 aacacctgga tatcaacacc gtggccgtct ttaactctgc tcacaccata gctgacatct
     2221 accaccacga cgaccgtatg gtaaggcagg cgcgcttttg ttcgctcacc gcgctgccct
     2281 ccgatcatat catgcttgca cacccgctcg ccgaccgcca ggcgatggtg atgcacagct
     2341 cgggactggt ccttcacggt cagggctatc ttttcgccgg gcagtccggg gcgggaaaat
     2401 cgaccgtcat caagctgctg cgcgactatg gctatgtgct gtgcgacgac cgggtagtgg
     2461 tgcggaaatg gccggaggga ttcaggctgc acggcacctg gagccacggc gagatcccgg
     2521 tgacttccaa tcttcaggcg cccttgaagg cgattctgct gcttaaaaag gcgcgccgct
     2581 gcaagatcga gccgatcctc gaccccaaag agatcatgca cgcgctttta attcgggtgg
     2641 cgcggccggt gcagaccgct gagtggctga aaaaagttct ggacctgctg ggcgccctgg
     2701 ccggcgaggt ccctgtctac cggctctcct tcaccaggga cgcgcggatc gtcgaagcca
     2761 tggaaaccct gaccgggccg ctgcaggccc ttaaaaccgc agcctccatt ccgccggtcg
     2821 aaacccgtca tgactagtcc cgctgcaagc gctccggcgc agggctatct ggcggagcag
     2881 cccgcgctgc gctggaatgg ggcccggccg ctgctcaccg tctttgatat cgaactaacc
     2941 gaacgctgca acaacaactg catccactgc tgcatcaacc tgccggaacg cgaccgcagc
     3001 gcccgcaagc gggagctatc cacggaggcc gtcgaggaac tgctcaggca gggggcggag
     3061 ctgggcgcac tgaccgtgcg cttttccggc ggcgagcccc tgctgcgtga tgatttcacc
     3121 gaactctatc tttacgcgcg ccggctggga ctgcgcgtca tgctcttcac caacgcacgg
     3181 ctgatcaccc cggaactggc cggcctcttc gcccgcatcc cgccgcgcga acccgtcgaa
     3241 ataaccgttt acgggatgtc tgccgattcc tgcgcagcgg tcacccgcag ccgcggcgcc
     3301 tttgccgaat tcaggcgcgg cgtcgatctg ctcatccgcc accggatccc ctttttcctc
     3361 aagggcacga tgctgcctga gaccagatcc gaactggagc agcttctcag ctggtcacag
     3421 agcatcccgg gtatgaaaga gctgccctcc tttgtgctct ttctcgagct gcgccatcgc
     3481 cgcgattcgg aggcgcgcaa ccggcagatt gaaaagctgc ggctctcccc ccgggaagcc
     3541 atcgaggttc tggccagaga ccccgatgta tacaaggccg atctgcaccg tctctgccga
     3601 tatgttgccg gcaaacccac cgacctgctc tttacctgca acgccggcaa ccagatctgt
     3661 atcgacgcct acggccaaat ccagtactgc ctggccctgc gccatcccgg cacggtcgtc
     3721 aacagcagca tctatccgct ggaattcgcc ctgcgcgaat ttttcccgca gctgcggcaa
     3781 atgcgagcga ccaatccgga ttttatcaac cgctgcgccc gctgcatgct gcgcggccta
     3841 tgcgaacagt gcccggccaa gtcgtggatc gaacatggcg ttctcgatca accagtggaa
     3901 tactgctgcg aggcggcgca cgaacaggcg cgctatctgg gcgtactgac aggggatgaa
     3961 aaaggctggg aagtggcgga ttggggggaa cggctgcgcc gtattaacgt gggagagggc
     4021 tgagcgttgg acggggattg aactgcgggg accggcgggg aactcactcc cgcccgggga
     4081 tgggctggtt gaacaattcg gcataggggc cgccggcgcg ccacaactcc tg
//
LOCUS       DAOPWU010000316         4122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_21834_length_4122_cov_5.569953, whole genome shotgun sequence.
ACCESSION   DAOPWU010000316 DAOPWU010000000
VERSION     DAOPWU010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21834_length_4122_cov_5.569953"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1382)
                     /locus_tag="PKM23_12860"
     CDS             complement(69..1382)
                     /locus_tag="PKM23_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017169564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNY92407.1"
                     /translation="MIPILHGLLGLLFVTLLAFLFSTHKKAIDWKLVLIGIALQFAFA
                     FLVLKTAAGRAVFAWLSGLFVALFSFAADGAQFVFGPLGRGSGEGSLGVIFAFQVLPT
                     IIFFAAFMAVLYHLGIMQWIVKGMAWVMQRFMRTSGAETLDVAANTFLGQTEAPLVIR
                     PYLSTLTESELYTIMASGMAHISGGVLGAYMQMLGIAMAKAQGVPVDQSQLFFAGHLL
                     AASIMAFPATMVVAKMLVPETGEPMTRGTVRLKVEKNSGNVIEAAAGGAGDGLRLALN
                     VGGMLIAFIALIALINALLGFIGSSTGLNSFLMMHFNKPLNLELLFGLVFQFIAWIIG
                     VPWKEALDVGSLMGIKLAVNEFVAYLKMSDVIAAGQLSTKSIVIATYALCGFANFASV
                     AIQIGGLSPLMENRRSDIARLGLKSVLGGTIATWMTAAIAGILTH"
     gene            complement(1506..3803)
                     /locus_tag="PKM23_12865"
     CDS             complement(1506..3803)
                     /locus_tag="PKM23_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92408.1"
                     /translation="MPANRTPSLLELETRALQLAGHLHSDASLESKRSRFFSFFLSAA
                     GFGDLASLRSYFLQFSPFLLPLAQGVTGCSRDPEELHRWLDVAVRFKSFIPDDAHDID
                     AAERTIRLAAILHALYAADHQLAARLLGLDGLDETVGALSRLDAARLVAGQAPSPLRE
                     ELLAGLQAWVAQTFAVSAHELRVLLIDESTLTDPGGSPVGVILPLHAEARERPSDAEE
                     DHALINNQATFGRQALYWTLQDGLLAARAHMSPGTARRFYQVHYSLPEKTAEVSGSSL
                     GLAAALLAWVTSRNRHYRTEVVRLAASAAVTGGISPNGKVTAVDSPTLAAKIRAAFFS
                     PAERLFLPAANIAEAWPLLSTLEQRYPRRRLLLQPIETLQQALQDRNLIADRKPGAPR
                     RALAALRRTRHKSFIAGIAAAAALGLLLATVPALQWWRDRQPALAELSEKEIIFKNQS
                     GERLWSHPLGFTLPTGDRDFTRIVQLLIDDLDDDGRREVFFGINVSAFPEECGILHSF
                     DSRGRALWPPLKLGKPMETRSGTRIRDHFYFSPILSVRIRAGQPKMLLFSIGSIHDFA
                     SRLVLIDSQGRVRGSYWNSGHIPVCAVHDLNGDGRMEIIAGLYGNEDGQARLAVFDTD
                     EMQGASPQSKPAYTLEEMAPARHLRLFRFPPSPFWEPGAYRDVVGRIESFGENLRADI
                     ANSGMVKESNESILNFRMYGYLFSTTLEPIALNDPPDRFLARFRDIFGRDFAPADRAR
                     LEVIDEWDGTAWHTRRIGPASSPPQ"
     gene            complement(3805..>4122)
                     /locus_tag="PKM23_12870"
     CDS             complement(3805..>4122)
                     /locus_tag="PKM23_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92409.1"
                     /translation="EKMEITTPRGVYDLSRTTSLSAAGDDKQQLILPGPPRAEIDLTI
                     DRQPQGIRYIVTRSGGASTPEADELDIVGITSKRILPVKTHKGVALLLVEPDEEMLKI
                     HIY"
BASE COUNT          808 a         1128 c         1468 g          718 t
ORIGIN      
        1 tgatatcccc ggagacgaac cagcggtagc ggggtgtttc attcttggtc atggatcctc
       61 cctcgttgtc aatgggtcag gatgccggcg atggcggcgg tcatccaggt ggcgatggtg
      121 ccgcccagca ccgatttgag gccgagccgg gcgatgtcgc tgcggcggtt ctccatcagg
      181 gggctgaggc cgccgatctg gatggcgacc gaggcgaaat tggcaaagcc gcacagggca
      241 taggtggcga tgacgattga tttggtcgag agctggccgg cggcgatgac atcggacatc
      301 ttgagatagg cgacgaattc attgaccgcc agtttgatcc ccatcaggct gccgacatcg
      361 agggcctctt tccaggggac gccgatgatc caggcgatga actggaagac gaggccgaag
      421 agcagctcga ggttgagggg cttgttaaag tgcatcataa gaaaactgtt gagtccggtg
      481 gagctgccga tgaagccgag cagggcgttg atgagggcga tcagggcgat gaaggcgatg
      541 agcatgccgc cgacgttgag ggcaagccgc agcccgtcgc cggctccgcc tgcagccgct
      601 tcgatgacgt tgccgctgtt cttctccacc ttgaggcgta ccgtgcccct ggtcatcggt
      661 tccccggtct cggggacgag catcttggcc accaccatgg tggcgggaaa ggccataatc
      721 gaggcggcga gcaggtggcc ggcgaagaaa agctgggact ggtcgaccgg caccccttgc
      781 gccttggcca tggcaatgcc gagcatctgc atgtaggccc ccagcacccc tccggagatg
      841 tgggccatgc cgctggccat gatggtatag agctccgact cggtcagggt cgaaagatag
      901 gggcggatca ccaggggcgc ctcggtctgg cctaaaaagg tattggcggc cacatcgagg
      961 gtctcggccc cgctggtgcg catgaagcgc tgcatcaccc aggccatgcc cttgacgatc
     1021 cactgcatga tgccgaggtg gtagaggacg gccataaagg cggcgaagaa gatgatcgtc
     1081 ggcagcacct gaaaggcaaa gatcacccca aggctgccct cgccgctgcc cctgccgagg
     1141 ggcccgaaga cgaattgggc gccgtcggcc gcaaagctga agagggcgac gaagaggccg
     1201 ctgagccagg cgaaaacagc ccggccggct gcggtcttga gcaccagaaa ggcgaaggcg
     1261 aattgcaggg cgatgccgat caggaccagc ttccagtcga tggctttttt atgggtggaa
     1321 aagagaaagg cgaggagggt gacgaagagc aggcccaaca ggccgtgcag gatcgggatc
     1381 atggcgatgg cgctccttgg ctggcggtta agggattcgc atgcgccaca atatagaaaa
     1441 tattttgcag ggatgcaaaa agttttcccg ggatcgggca ggaagtatgg ctggaacggc
     1501 cggaattact gcgggggtga agaggctgga ccgatgcggc gggtgtgcca ggccgtgccg
     1561 tcccactcat cgatgacctc gagccgggcg cggtcggctg gagcaaagtc gcggccaaaa
     1621 atatcgcgga agcgggcaag gaaacggtcg gggggatcgt tgagggcgat cggttccagg
     1681 gtggtggaaa ataaatagcc gtacattcta aagttcagaa tgctctcatt cgattccttg
     1741 accatgcccg aattggcgat atcggcgcgc agattttcac cgaaggactc gatacgcccc
     1801 acaacatcac ggtaggcgcc gggttcccag aaaggggagg ggggaaagcg gaagaggcgg
     1861 aggtggcggg cgggtgccat ctcctccagg gtgtatgccg gcttgctttg cggcgaggcc
     1921 ccctgcatct catcggtatc gaatacagcc agccgggcct gtccatcctc attgccgtag
     1981 agtccggcga tgatctccat gcggccatcg ccgttgaggt cgtggaccgc gcagaccggt
     2041 atatggcctg aattccagta ggagccgcgc acccggccct ggctgtcgat cagcaccagt
     2101 ctggaggcga aatcgtggat ggagccgatg ctgaagagga gcattttagg ctgtccggcc
     2161 cgaattcgca cgctgaggat cgggctgaag tagaaatggt ccctgattct cgttccgctc
     2221 ctggtctcca tgggcttgcc cagtttgagg ggcggccaaa gggctcgtcc ccggctgtca
     2281 aagctgtgca ggattccgca ctcttcagga aaggcagaaa cattgatgcc gaagaagacc
     2341 tcacgccggc cgtcgtcgtc cagatcatcg atgagcagct gcacgatccg ggtgaaatct
     2401 cgatcgccgg tgggcagggt gaatcccagc gggtgcgacc agagccgctc tccggactgg
     2461 ttcttgaaga tgatctcctt ttcagagagt tcggcgaggg ccggctggcg gtcgcgccac
     2521 cactgcaggg ccggcacagt ggcaaggagc agccccaatg ccgcggcggc ggcgatgccg
     2581 gcgatgaagg atttatgccg ggtacggcgc agggcggcca gggcgcgccg gggcgcaccc
     2641 ggtttgcgat ccgcgatcag gttgcggtcc tggagggcct gctgcagggt ctcaatcggc
     2701 tgcaggagca gtcgacggcg gggatagcgc tgctcgagcg ttgacaatag gggccaggcc
     2761 tcggctatat tggccgccgg caggaagagg cgctcggccg gggagaaaaa ggcggcgcgg
     2821 atcttggcgg ccagagtggg gctgtcgacg gcggtgactt tgccgtttgg gctgataccg
     2881 ccggtgaccg ctgcggaggc ggcgaggcgc acgacctcgg tgcggtagtg gcggttgcgg
     2941 ctggtgaccc aggccagcag ggcagccgcc agaccgaggg aggaaccgct cacctccgcc
     3001 gttttttccg gcaaggaata atgcacctga taaaaacgcc gtgctgtgcc cggggacatg
     3061 tgggcccgcg ctgccagcag gccatcctgg agggtccagt acagggcctg gcgtccaaag
     3121 gtggcctgat tgttgatcag ggcatgatcc tcttcggcgt cggaggggcg ttcgcgagcc
     3181 tcggcatgca ggggcaggat gacgcccacg ggcgagccgc cgggatcggt gagggtgctc
     3241 tcgtcgatga gcaggaccct gagttcgtgg gcggaaacgg caaaagtctg cgccacccag
     3301 gcctgcagcc ctgccagcag ttcttccctg agcggtgagg gggcctggcc ggccaccagg
     3361 cgggcggcat ccagtcgcga aagcgctccg accgtctcat ccaggccatc gaggccgagc
     3421 agacgggcgg caagctgatg gtcggcggcg taaagggcgt gcaggatggc ggccaggcgg
     3481 atggtgcgtt ctgcggcgtc gatgtcatgg gcatcatccg gaatgaacga tttgaagcgg
     3541 accgccacat caagccagcg gtgcagctcc tcggggtcgc ggctgcagcc ggtcacaccc
     3601 tgcgccaaag gcaggaggaa agggctgaac tgcaggaaat aggagcgcag ggaagccaga
     3661 tctccaaatc cggcggcgga gaggaaaaat gagaagaaac ggctgcgttt gctctcgagg
     3721 gaagcatcgc tgtgcaggtg tccggcgagc tggagcgcac gggtctcgag ctcgagcagg
     3781 gagggggtgc ggtttgcggg catgtcagta gatatgaatt ttcagcatct cttcgtccgg
     3841 ctcgaccagc agcagggcaa cgcccttgtg ggtcttgacc gggagaatgc gtttgctggt
     3901 gatgccgacg atatccagtt catctgcttc aggtgtggat gctccacctg accgggtgac
     3961 gatatagcgg atgccctgcg gctggcggtc gatggtcaga tctatctccg cccggggcgg
     4021 gccgggcaga atcagctgct gcttatcgtc tccggcagca gataaagagg tggttcggga
     4081 gaggtcatag acgccgcgcg gcgtggtgat ctccattttt tc
//
LOCUS       DAOPWU010000317         4096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_22017_length_4096_cov_4.552586, whole genome shotgun sequence.
ACCESSION   DAOPWU010000317 DAOPWU010000000
VERSION     DAOPWU010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22017_length_4096_cov_4.552586"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="PKM23_12875"
     CDS             <1..1561
                     /locus_tag="PKM23_12875"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNY92410.1"
                     /translation="SPHSALILALIAGLMITYLLSQGIDITLRGEKPFTWMPISAGLA
                     PLFAGMMPVQLAIWNHLFWWSHLLMILGFLVIIPGSKHLHLLSGPVNLFFRSREPVGT
                     LQPIDFEKSEQFGAATITDYPWKNISDLFACIDCGRCQDLCPAYATGKPLSPKVVMMS
                     QRTYALAESRALLAGQAPSVSIVPAALSEDEIWSCTTCGACMSACPVLNEHIPAIVDL
                     RRSRVMMESTFPQELQGAFRGLETNANPWNAGSSTRMEWAEGLEIPLMAEIGEAEYLW
                     YVGCAGAFDDRARAISRSLALILRHAGIRFGVLGLEEKCCGDPARRTGNEYLFQSLAM
                     ENIQILQQYKFRYLLTSCPHGFHVFHDEYRRLGAEFEVVHHSELIRQLLVEGRLRLKE
                     GGSGRITWHDSCYLGRYHQIYAAPRQVLTTLNGTPPLEMARHHERSFCCGGGGGGMFM
                     EERLGSRISHSRIAEAVAAKAQIVASGCPFCLTMLDDAIREKGLAEQMRTGDLAQLVA
                     ERLAGDYPHPV"
     gene            complement(1630..>4096)
                     /locus_tag="PKM23_12880"
     CDS             complement(1630..>4096)
                     /locus_tag="PKM23_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNY92411.1"
                     /translation="VNLPSIVNVPDLSRPEDDPDFYMRVELDRANDTGERIIVTYTIT
                     PGTAAYPADYSGPDASVRTVPIPLNATNRGLAPVLTIDDNIYEASETFTVTIISARTE
                     NSNLPIAVLDNSATGTITDNDPRPTVSIVNDGADEGDSPENDVPDENRGRVRVELSNP
                     ADRDVLVDYYTSDGTALAADNDYQPASGTLVIPAGETMKRIRIIYVADNKVEPDEHFF
                     VHLSSAAFGPDNGLEIIDSRARGRILNDDFTADIEVAAKWVREADGGPKLSTVTAGEV
                     YRFVIQIFNRGDADAEDVVIEDVLPAGLTFQYADHGGSFDGSKVTWTLSSILRNQEKR
                     VRVYALVDADVPEGTEITNSGCRVAGLIPDLNNENDCAEVTVLVLQPDVDQVANINSD
                     INVGQAPLLARFTSMKPLTSYKWWLDLDKRSDWDHPSYLYRHVVPPAGPGKNYDITLE
                     GPVETEEFEDMMRVYGPGGYGHLQVVSGSPTTSAGSWSNAVDGDLYWYDGTAEVAADA
                     SGAAWAIFEFTDQSVRKVNQVRMMTDTGIDNLKKQATEFKVLVSTDGVTFTEVLAATR
                     NNNVNTPALLHDDWMSWSFEPIDARFVKLMVTAPLAYTYAAIGEFEVWFEAKLADPGQ
                     STLTVSGETVSMTIKDALGSPITGLTHHDIIVYSFNTDSFYGDPRFAMGTGLTEDSAN
                     PGTYTCSIQNKGVVEASVNGVIIPKPVAAQAAFQADAVPVAESAAAALQALPTEFALE
                     QNYPNPFNPETTIRYQLPEGVQVTLKVYDLRGRELETLVNQFQPAGFHSASWRTLNHA
                     SGVYFYHLTAGSFKAIRSMTLVR"
BASE COUNT          735 a         1208 c         1320 g          833 t
ORIGIN      
        1 ctccccccac agcgccctca tcctcgccct gattgcgggc ctgatgatca cctatctgct
       61 cagccagggg atagatatca cgctgcgcgg ggagaagccc ttcacctgga tgccgatatc
      121 ggcgggtctg gcgcccctct tcgccgggat gatgccagta cagcttgcaa tctggaatca
      181 tcttttctgg tggtcccatc tcctcatgat cctgggattt ctggtcatca tccccggcag
      241 caaacatctg catctcctct ccggcccggt caatctcttt ttccgcagcc gcgaacccgt
      301 cggtaccctg cagcccatcg actttgaaaa gagcgagcag tttggggcgg ccacgatcac
      361 cgattatccc tggaagaaca tcagcgatct ttttgcctgc atcgactgcg gccgctgtca
      421 ggatctctgc ccggcctacg ccaccggcaa gcccctctcg cccaaagtgg tgatgatgag
      481 ccagcgcacc tacgccctgg ccgagtccag ggccctgctc gccggccagg cgccctccgt
      541 gtcgatcgtg cccgctgccc tcagcgagga tgagatctgg tcctgcacga cctgtggggc
      601 ctgcatgtcg gcttgcccgg tgctcaacga gcacattccc gccattgtcg atctgcgccg
      661 aagccgggtg atgatggaga gcacctttcc ccaggagctc cagggcgcct tccgcggcct
      721 cgagaccaac gccaacccct ggaacgccgg cagcagcact cgcatggagt gggcggaggg
      781 actggaaata ccgcttatgg ccgagatcgg cgaggcggaa tacctctggt atgtcggctg
      841 cgccggggcc tttgatgacc gcgcccgggc gatcagccgc agcctcgccc tcattctccg
      901 ccacgcgggc atccgcttcg gcgtgctcgg cctcgaggag aaatgctgcg gagacccggc
      961 aaggcgcacc ggcaatgaat acctttttca aagtctggcc atggagaata tccagatcct
     1021 gcagcagtac aaattcaggt atctgcttac ctcctgcccc catggctttc atgtctttca
     1081 cgacgagtac cgccgtttgg gtgctgagtt cgaggtcgtc catcacagcg aactgatccg
     1141 tcagctgctg gtggagggga ggctccggct aaaggagggc gggtccggcc gcatcacctg
     1201 gcatgattcc tgctatctgg gccgctatca tcagatctat gccgcgccgc gccaggttct
     1261 gaccactctc aacggaacgc cccccctgga gatggcccgc catcacgaac ggagcttttg
     1321 ctgcggcggc ggaggaggtg gcatgtttat ggaggagcgc ctcggcagcc gcatcagcca
     1381 cagccgcatt gcggaggccg tcgcagccaa agcacaaatt gtcgccagcg gctgtccctt
     1441 ctgcctgacc atgctcgatg atgccattcg cgaaaaaggg ctcgcagagc agatgcggac
     1501 gggcgatctc gcccaactgg tggcggaaag gctcgccggg gattacccgc atcctgtatg
     1561 acttttcccc gaatgtgaca aaaaaaaggg ctgcctttgg gcagcccttt tttttaatgg
     1621 cgtctacgtc tagcgcacca gggtcatact tctgatcgcc ttgaaggagc cggcggtgag
     1681 gtggtaaaag tagacgccgc tggcgtggtt gagggtccgc caggaggccg aatggaaacc
     1741 ggcgggctgg aactggttga ccagagtctc cagttctcgg ccgcgcaggt catagacctt
     1801 gagcgtgacc tgcacccctt ccgggagctg atagcggatg gtggtctccg gattgaaggg
     1861 gttgggatag ttctgctcga gggcgaactc tgtgggcaga gcctggagtg cagccgcggc
     1921 gctttcggcg accggaacgg catcggcctg gaaagcggcc tgggcggcga ccggtttcgg
     1981 gatgatcacg ccgttgaccg aagcctcgac cacgcccttg ttctgaatcg agcaggtgta
     2041 ggtgcccgga ttggcgctat cttcagtcag cccggttccc atcgcgaagc ggggatcgcc
     2101 gtaaaagctg tcggtgttga agctgtagac gatgatgtca tgatgggtca agccggtgat
     2161 cgggctgccc agggcatcct tgatagtcat ggagacggtc tcgccgctga cggtcagggt
     2221 cgactgcccc ggatcggcca gtttggcctc gaaccagacc tcaaactcgc cgatggcggc
     2281 gtaggtgtac gccagggggg ccgtgaccat cagcttgacg aaacgggcat cgatcggctc
     2341 gaagctccag ctcatccagt catcgtggag cagggcaggg gtgttgacat tgttgttcct
     2401 ggtggcggcg agcacctcgg tgaaggtgac gccatcggtg gagaccagca ccttgaactc
     2461 cgtcgcctgc ttcttgagat tgtcgatgcc ggtatcggtc atcatccgca cctgattgac
     2521 cttgcggacg gactggtcgg tgaactcgaa gatggcccag gcggcgccgg aggcatcggc
     2581 ggccacttcg gccgtgccgt cgtaccagta aagatcgccg tcgactgcgt tgctccagga
     2641 gccggcgctg gtggtcggcg aaccgctcac cacctgcaaa tggccatagc cgcccggacc
     2701 gtagacgcgc atcatgtcct cgaactcctc ggtctcgacc gggccttcca gggtgatgtc
     2761 atagttcttg ccgggaccgg cagggggcac gacatgacgg tagaggtagg agggatggtc
     2821 ccaatccgag cgcttgtcga gatccagcca ccacttgtag ctggtcaggg gcttcatcga
     2881 ggtgaagcgc gccagcagag gggcctggcc gacgttgatg tcggaattga tgttggcgac
     2941 ctgatcgaca tcgggctgga gaaccagtac ggtgacctcg gcgcagtcat tttcattgtt
     3001 gaggtcggga atcagccccg cgaccctgca gccgctgttg gtgatttcgg ttccttccgg
     3061 cacatcggca tcgacgaggg cgtagacgcg aacccgcttc tcctggttgc ggaggatgga
     3121 gctcagggtc caggtgacct tgctgccgtc aaagctgccg ccgtggtcgg catactggaa
     3181 ggttagcccg gcggggagca catcctcgat gaccacatcc tcggcatcgg catcgccgcg
     3241 gttgaaaatc tggatgacga agcggtagac ctcaccggcc gtgactgtgg aaagcttggg
     3301 gccgccgtca gcctcgcgca cccatttggc cgccacttcg atgtcggctg tgaaatcgtc
     3361 gttgagaatg cggccgcggg cgcggctgtc gataatttcc agtccattat cagggccgaa
     3421 ggcggcgctg ctgagatgga cgaaaaaatg ctcatccggt tcgaccttgt tgtcggctac
     3481 ataaatgatg cggatccgtt tcatggtttc gccggcggga atgaccagag tgccactggc
     3541 gggttgatag tcattatccg cagccagggc agtgccatcg gaggtatagt aatccaccag
     3601 gacatccctg tcggctggat tggagagctc gacccggacg cggccgcgat tttcgtcggg
     3661 cacgtcgttt tcggggctgt cgccctcatc cgcaccgtcg ttgacgattg aaaccgtggg
     3721 ccggggatcg ttatcggtga tggttcccgt ggcgctgttg tcgagcacag cgatgggcaa
     3781 attgctgttt tcggtccggg cgcttatgat ggtgacggtg aaggtctcgc tggcctcata
     3841 gatgttgtca tcgatggtga ggacaggcgc cagaccgcgg ttggtggcgt tgaggggtat
     3901 cggcacggtg cgtacgctcg catcggggcc ggaatagtcg gccggatagg cggcggtgcc
     3961 gggggtgatg gtgtaagtga cgatgatgcg ctcgccggta tcattggccc ggtcgagctc
     4021 gacccgcatg tagaaatcgg ggtcatcctc cgggcggctg agatccggaa cattgacgat
     4081 cgagggcaga ttgacc
//
LOCUS       DAOPWU010000318         4095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_22020_length_4095_cov_3.829703, whole genome shotgun sequence.
ACCESSION   DAOPWU010000318 DAOPWU010000000
VERSION     DAOPWU010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22020_length_4095_cov_3.829703"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="PKM23_12885"
     CDS             complement(<1..1207)
                     /locus_tag="PKM23_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNY92412.1"
                     /translation="MAEADSGGTTIRSDTLRLSLGYRLRPRLFAWATAPGKSIRLHSR
                     VMENPVSAPLSYQWQADPGNPAAVNLLTPGDSTTLVTINCDGLPGEYYFNLTAVTPAG
                     DTARARTFITLDQSGIRPFRIETDHAAWIDGAILYEITPCSFVQDGRLADITAKIPEL
                     AELGVTALWLQPTFGTLDFGGMGYGITDYFSVRSDLGDEADLRHLVATAHAQGLRVLL
                     DMVPNHSHIRHPYALDAVRFGTCSHYYDFYQRSAEVLPEVPYSQYYNLHAQGFVYYFW
                     DELPNLDYDNPEVRRWMTEICTYWVREFDIDGYRFDAVWGVNARRPDYTRELRLALKE
                     IKPELLMLAEDKAPSARVFDGRFDLAFDWSADDGWVSQWSWQVLYHDYWQDQYVTIFN
                     SWQPGRSLRL"
     gene            complement(1471..2619)
                     /locus_tag="PKM23_12890"
     CDS             complement(1471..2619)
                     /locus_tag="PKM23_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNY92413.1"
                     /translation="MTSKERLLITMKGGVADHVPVSPDISVMVPDRLDGRPFYDIHLD
                     GREHFGWTSATHGEVYVKAVQYFGCDGFYMYGGLKQIKPDSRPEFESRISDAPQGKLV
                     RRICHTPLGDLTEEELFFADEPPWVGVKPLKELSTDFKKLKWMMGESGWKWEPAYRDK
                     EVIGDYGVYMGMVPVFQDWFFRNRQGGFEQMFVDYMMEPALVEEIHEFWMEWALANVR
                     AMISAEADVIMLGGSSASLSVSSPKLFRQFELPFIQKASVICREAGIVSHLHVCGRSN
                     ELVEIVAEESDLASIEPLEEPPSGNVNLKEIKRRFGKTMCLKGNINTTHFMLHATPEQ
                     IEEKCKRLIDDAGEGGGFILSTGDQCGRDTPEANLFKMVEVATTYGKY"
     gene            complement(2621..>4095)
                     /locus_tag="PKM23_12895"
     CDS             complement(2621..>4095)
                     /locus_tag="PKM23_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNY92414.1"
                     /translation="GVKEAGRAGYEVTRDGVVVPADIGTGRFSVNARWYVEGFGYLYL
                     SADNAGEFYSVREGGRFNLNHEFARSRVMRNRAVLQRYEKEGCRFTAEVRHLAGLSEE
                     LLADAAQKITSGETAARLSDRSLYYALQAGELIELEKARYEVARQARKEAFYFGCETR
                     QFIWAKHDEFIKRFAELFNFATVTHYIWDSWYEHFEPHEGEYNWGIKDDIVDWLSGNG
                     ITIQGRPLFWFHPIVTPDWLKEKNFEELKSYVVRHAGNLVKHYGDRVQQWEVVNEYHD
                     WANIHNHTPEQTTEIVRLACDATRAANPRVVRILNNCAPWAEYAAWGRMARQKELSSR
                     PLRSPQRFIQDLVEAGVEFDVLGIQVYFPRRDLSDIMRMIERFEKFGKPIYITELGTT
                     SGPTADLVATGKMSLPSEPFEWHRPWDEELQADWLEQVYSLYYSRPLIKAVNWYDFAD
                     FRTHILNGGLVREDGSPKRSFMRLQKLLSTWDRLPNTQRK"
BASE COUNT          849 a         1141 c         1244 g          861 t
ORIGIN      
        1 gaaggcgcag gctgcgtccc ggctgccagg aattgaaaat ggtgacatat tggtcctgcc
       61 aatagtcgtg atagaggacc tgccacgacc actgtgaaac ccagccatcg tccgcgctcc
      121 agtcgaaagc caggtcaaaa cgcccgtcga agacgcgagc ggatggggct ttatcttccg
      181 cgagcatcag cagttccggc ttgatctcct tcagggccag acgcagttcg cgggtgtaat
      241 cggggcggcg ggcgttgacg ccccagaccg cgtcaaaacg gtagccgtcg atgtcgaact
      301 ccctgaccca gtaggtgcag atttccgtca tccagcgccg gacttctgga ttgtcgtaat
      361 caaggttggg cagttcatcc cagaaatagt agacaaaacc ctgggcgtgg aggttgtaat
      421 actgcgagta gggcacctcg ggaaggactt ccgcgctgcg ctgatagaaa tcgtaataat
      481 gcgagcaggt gccaaagcgt accgcatcaa gggcgtatgg atgcctgata tgggagtggt
      541 tggggaccat gtcgaggagc acgcgcaagc cttgcgcgtg ggcagtggcg acgagatggc
      601 ggagatctgc ctcatcgccc aaatcactgc gcactgaaaa atagtcggtg atgccatagc
      661 ccatgccgcc gaaatcgagg gtgccaaagg tcggctgcag ccaaagcgcc gtcacaccta
      721 actccgccaa ttcaggtatc tttgcggtga tgtccgccag tcgaccgtcc tgcacaaaag
      781 agcagggggt gatctcgtag aggatcgccc cgtcaatcca cgcggcatgg tcggtttcga
      841 tccggaaggg ccggatgccg ctctgatcga gggtgatgaa ggtgcgggca cgggcggtat
      901 ccccggcagg cgtgaccgca gtcagattga aatagtactc tcccgggagg ccgtcgcagt
      961 tgatggtgac cagggtagtg ctgtcgccgg gtgtgagcag gttcactgca gcggggttgc
     1021 cgggatccgc ctgccactga tagctgaggg gggctgaaac gggattttcc atcacacgac
     1081 tgtggagccg gatgcttttt cccggggcgg tggcccaggc gaagaggcgc ggtcgcagcc
     1141 tgtagcccag gctgagtctc agggtatcgg agcggatggt ggtcccgccg ctgtctgctt
     1201 cggccacgag cgtattgaca ccttcagtga gtgtgacagt tacagaaaaa gtgccctccg
     1261 cagggctgac agcgaaggga atccgttctc tgttgagaga aagaataccc cggaccggag
     1321 caaagcctgt gaatgaaccc ctgatgacct gactctgccc ccagactgta gcatcatttt
     1381 tgtgcaggat aatttcggca tgcccattga ggtgaagcag gccgagcaaa acgatgagaa
     1441 ctgcgggtgc cttcattgca tctcccggct tcagtacttg ccataggtcg tggcgacttc
     1501 caccatcttg aagagattgg cctcgggggt atcacggccg cactggtcgc cggtggagag
     1561 aatgaatcct cccccctctc cggcgtcatc gatgaggcgt ttgcatttct cctcgatctg
     1621 ttccggcgtg gcgtgcagca taaagtgtgt ggtattgata ttgcctttaa ggcacattgt
     1681 tttcccgaag cggcgtttga tctccttaag gtttacatta cccgaaggag gctcttcgag
     1741 gggctcgatg gaggcgaggt cggactcctc ggccacgatc tccaccagct cattggagcg
     1801 gccgcagaca tgcagatggg agacgatccc cgcctccctg cagatgacgg aggctttctg
     1861 gataaagggg agctcgaatt ggcggaagag tttgggtgag ctgacgctga ggctggcgct
     1921 ggagccgccg agcatgatga catcggcctc ggcgctgatc atggcgcgta cattggcgag
     1981 ggcccactcc atccagaact cgtggatctc ctcgaccagc gcgggttcca tcatatagtc
     2041 gacgaacatc tgctcaaaac cgccttgccg gttgcggaag aaccagtcct gaaagactgg
     2101 caccatgccc atataaacgc cataatcgcc aatcacctcc ttgtcacggt aggccggttc
     2161 ccacttccag ccgctctcac ccatcatcca tttcagtttt ttaaagtcgg tagatagttc
     2221 cttgaggggt ttgaccccca cccagggtgg ttcatcggca aaaaagagct cttcctcggt
     2281 gagatcgccg aggggggtat ggcagatgcg gcgcaccagc ttgccctgcg gagcgtcgga
     2341 gatgcggctt tcaaactcgg ggcggctatc gggcttgatc tgcttgagtc cgccatacat
     2401 gtaaaaacca tcgcagccga aatactgcac agccttgacg taaacctcgc cgtgggtcgc
     2461 tgaagtccag ccgaagtgtt cacgtccatc gagatggatg tcataaaagg gtcgtccgtc
     2521 gaggcggtcg ggaaccatca cggagatatc cgggctgacc ggcacatggt ccgccactcc
     2581 gcccttcatg gtgatgagga gtctctcttt agaggtcatt ttatttcctt tgtgtgttgg
     2641 gcagccggtc ccatgtgctg aggagcttct ggaggcgcat gaatgaccgt ttcggagagc
     2701 cgtcctcccg gaccagtccg ccgttgagaa tatgggtgcg aaaatcagca aaatcgtacc
     2761 agttgaccgc cttgatcaac ggacggctgt agtaaaggga gtagacttgt tccagccaat
     2821 cggcctggag ctcctcgtcc catggccggt gccattcaaa cggttcggag ggcaggctca
     2881 tcttgccggt tgccaccaga tcggcggtag gcccgctggt ggtgcccagt tcggtgatat
     2941 agatgggctt gccgaacttt tcaaaacgct cgatcatgcg catgatatcg gagaggtcgc
     3001 ggcgcggaaa atagacctgg atgccaagaa cgtcgaattc gaccccggcc tcgaccagat
     3061 cctggatgaa acgctgcgga gagcgcagag gacgactgga gagttctttc tgccgcgcca
     3121 tccggcccca ggcggcatat tcagcccagg gcgcacaatt gttgaggatg cgcacgacgc
     3181 ggggattagc cgccctggtg gcatcacagg cgaggcgaac gatttccgtg gtctgttcgg
     3241 gcgtatggtt gtggatgttg gcccagtcgt ggtattcgtt gacgacctcc cactgctgca
     3301 ccctgtcgcc gtaatgtttg accaggttgc cggcatggcg caccacatag ctcttcagct
     3361 cctcaaaatt cttctccttc aaccaatcag gtgtgacgat gggatgaaac cagaaaagcg
     3421 gccggccctg gatggtgatg ccattcccgc tcagccagtc gacgatatcg tctttaatcc
     3481 cccagttata ctccccctca tgaggttcga aatgctcgta ccagctgtcc cagatatagt
     3541 gcgtgactgt ggcgaagttg aagagttcgg caaagcgttt gatgaattca tcatgtttgg
     3601 cccagatgaa ctgccgggtt tcgcagccga aataaaaagc ctccttgcgg gcttggcggg
     3661 cgacctcgta ccgggctttc tccagttcga tcaattcgcc ggcctgcagg gcatagtaga
     3721 gggagcgatc tgagagcctt gcagctgttt ccccactggt gatcttttgg gcggcatcgg
     3781 ccagcagctc ctcgctaagg ccggcgaggt gacggacctc tgcggtaaag cggcagccct
     3841 ccttctcata gcgctggagg actgcacggt tgcgcatgac ccggctgcgg gcaaactcat
     3901 gattgagatt gaaacggccg ccctcccgga ctgaataaaa ctctcctgca ttgtcggcgc
     3961 tgaggtagag gtagccaaag ccctcgacgt accagcgcgc attgacagag aaacggccgg
     4021 tcccgatatc cgccgggacg acgacaccat cgcgggtgac ctcgtagccg gctcggcccg
     4081 cctccttgac gcctt
//
LOCUS       DAOPWU010000319         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_22159_length_4077_cov_3.852810, whole genome shotgun sequence.
ACCESSION   DAOPWU010000319 DAOPWU010000000
VERSION     DAOPWU010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22159_length_4077_cov_3.852810"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2231
                     /gene="ppdK"
                     /locus_tag="PKM23_12900"
     CDS             <1..2231
                     /gene="ppdK"
                     /locus_tag="PKM23_12900"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNY92415.1"
                     /translation="IRHQLEHIMAKLKKAKGYDSDTDMTAADLKKLVEEFKAKIKEVL
                     GKSFPDDARKQLWGGINAVFQSWNGRRAISYRRIEGIPDEWGTAVNVQAMVFGNMGDT
                     SATGVAFTRNPATGENKFYGEWLINAQGEDVVAGIRTPAPLNEATKNEQNKHMASLQK
                     AMAKTYTQLDKIRLGLEGHFKDMLDIEFTIQEGKLWMLQCRVGKRTGTAALNMAMDML
                     KEKMIDEKTAVMRLAPAQLDELLHPIVDPAAEKKAAPLAKGLPAGPGGATGQIVFTAA
                     DAVAWAKAGKKVLLVREETNPEDVEGMRAAEGILTGRGGMTSHAALVARGWGKCCIVG
                     CSALHIETAKKILKIDGKEYKEGDWFTLNGTRGHVYPGQLAMMSASENPRFLKFMKLV
                     DKNRRMKVRTNADTPEDAKKALEFGAQGIGLFRTEHMFYGKNSEKPLFALRKMIISKT
                     EAERRKAVDELFPYVKKSIKETMEVMNGLPVTIRLLDPPLHEFVPQEDNKRTELAKAL
                     GIDKAEFVKRVEALHETNPMMGHRGVRLGVTFPEITEMQIRAILEATVELVKAGIKVL
                     PEIMVPVVCHERELVDQRAIVEQVYKEVCAKYKVKAIQYMYGTMIEIPRAALQAGKIA
                     GAAEFFSFGTNDLTQMGFGFSRDDIGGFVPDYVEKRILPADPFNILDQEGIGELIEIG
                     IERGRKARPGLKVGICGEHGGEPASVEFCHRAGMDYVSCSPYRVPIARLAAAQATIRE
                     KK"
     gene            2315..2971
                     /locus_tag="PKM23_12905"
     CDS             2315..2971
                     /locus_tag="PKM23_12905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF018583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HNY92416.1"
                     /translation="MSTQLRIFCDFDGTIAQRDVGNLVFGHFGSESHWWGLVELWQQG
                     RLEGREMWRRQAEVMRLTETELHEFLAPLAIDTSFGMLAGFARQEGIPVTIVSDGMDA
                     YIAPILAAHGFGDLPLRTNRMVLTPEGAVRVEFPWSGQGCGQCANCKGLHVRRERLPG
                     ETTVYIGDGPSDICGAREADIVFAKKQLLAWCRENSHPHYPFTSFADIHDHLQGLLKR
                     "
     gene            3060..>4077
                     /locus_tag="PKM23_12910"
     CDS             3060..>4077
                     /locus_tag="PKM23_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNY92417.1"
                     /translation="MEIQFFGGARSVTGTMHVLIVRGKKILLDCGLYQGKRDEAFERN
                     RQLPFDAASIDAMVLSHAHIDHSGNIPQLVKLGFTGNIVCTHATRDLCSIMLQDSGYI
                     QEKDAEYVNKRRARKGQRPVEPLYTARDAVKAMNQFFSVGYERKVQIADGVSVTFHDA
                     GHILGAAISALDINDDGKSIRLVFSGDLGRPGQPILRDPAILREADILFTESTYGGRF
                     HDDFATLNQKLAQVVNTTVNRGGKIIVPAFSVGRTQEIVYALHQLTLAGEIPELPIFV
                     DSPLSLNATEVFRAHPECYDEETADFLESTDDVFGWNRLRYIRTAEESKKINEIKSPC
                     IIISA"
BASE COUNT          847 a         1331 c         1182 g          717 t
ORIGIN      
        1 gcatccgcca tcagctcgag cacatcatgg ccaaactgaa aaaggccaag ggctatgact
       61 ccgataccga catgaccgcc gcagacctca agaaactggt cgaggagttc aaggccaaga
      121 tcaaagaggt gctgggcaag tcctttcccg atgatgcccg caagcagctc tggggcggca
      181 tcaacgcggt cttccagtcc tggaacggcc gccgcgctat ctcttaccgc cgcatcgagg
      241 gcattcccga tgaatggggc accgccgtca acgtccaggc catggtcttc ggcaacatgg
      301 gcgacacctc cgccaccggc gtggccttca cccgcaaccc ggccaccggc gagaacaagt
      361 tctacggcga gtggctgatc aacgcccagg gcgaggacgt cgtcgccggc atccgcaccc
      421 cggcgccttt gaacgaggcg accaagaacg agcagaacaa gcatatggcc tcccttcaga
      481 aagccatggc caagacctac acccagcttg acaagatccg cctcggactc gagggtcatt
      541 tcaaggacat gctcgacatc gagttcacca ttcaggaagg caagctctgg atgctgcagt
      601 gccgcgtcgg caagcgcacc ggcaccgcgg ccctcaacat ggccatggat atgctgaaag
      661 agaagatgat cgatgaaaag accgcggtca tgcgcctcgc gcccgcccag ctcgacgaac
      721 tcctccatcc catcgtcgat cccgccgccg agaaaaaggc cgccccgctc gccaagggtc
      781 tgcctgcggg tcccggcggc gccaccggcc agatcgtctt caccgcggcg gatgccgtgg
      841 cctgggccaa agcaggcaag aaggtcctgc tcgtccgcga agagaccaat cctgaagacg
      901 tcgaaggcat gcgcgcggcc gagggcatcc tcaccggacg cggcggcatg acctcccacg
      961 ccgctctcgt cgcccgcggc tggggcaagt gctgcatcgt cggctgctcc gctctccata
     1021 tcgagaccgc caaaaaaatc ctcaagatcg acggcaagga gtacaaggaa ggcgactggt
     1081 tcaccctcaa cggcacgcgc ggccatgtct accccggcca gctggcgatg atgagcgctt
     1141 ccgaaaatcc ccgcttcctc aaattcatga agctggtgga caagaaccgg cgcatgaagg
     1201 tgcgtaccaa cgcggatacc cccgaggacg ccaaaaaggc cctcgagttc ggcgcccagg
     1261 gcatcggcct cttccgcacc gagcacatgt tctacggcaa gaattcggag aaacccctct
     1321 ttgccctgcg caagatgatc atctccaaga ccgaggccga gcgccgcaag gcggtcgatg
     1381 agctcttccc ctatgtcaag aagagcatca aggagaccat ggaggtcatg aacggcctgc
     1441 ccgtcaccat ccgcctcctc gatcccccgc tccatgaatt cgtgccccag gaggacaaca
     1501 agcgcactga gctggccaag gcgctgggta tcgacaaggc cgagtttgtc aagcgcgtcg
     1561 aggccctgca cgaaaccaac ccgatgatgg gccaccgcgg cgtccgcctc ggtgtcacct
     1621 tcccggagat caccgagatg cagatccgcg ccatcctcga ggcgaccgtg gagctggtca
     1681 aggccggcat caaggtgctg cccgagatca tggttccggt ggtctgccat gaacgcgagc
     1741 tggtcgacca gcgggcgatc gtggaacagg tctacaagga ggtctgcgcc aaatacaagg
     1801 tcaaggccat ccagtacatg tacggcacca tgatcgagat tccacgcgcg gccctgcagg
     1861 ccggcaagat cgccggcgca gccgagttct tctccttcgg caccaacgac ctgacccaga
     1921 tgggctttgg cttctcccgc gacgacatcg gcggcttcgt ccccgattac gtcgagaaga
     1981 gaatcctgcc ggccgatcct ttcaatatcc tcgaccagga gggcatcggc gagctgatcg
     2041 agatcggcat tgagcgcggg cgcaaggcgc gtccgggcct caaggtcggc atctgcggcg
     2101 aacacggcgg cgagcccgct tcggtggagt tctgccaccg cgcgggcatg gattatgtct
     2161 cctgctcgcc ctatcgcgtc cccatcgccc gtctggctgc ggcccaggcg acgatccgcg
     2221 aaaagaagta aatcatttgt aaaccgtcac cagccccggg gctccgaaag ccccggggtt
     2281 ttttatcgtc cctcggtgag agctaccctt aaccatgtcc acacaactgc gcatattttg
     2341 tgattttgac ggcaccatcg cccaacgcga tgtcggcaac ctcgtcttcg gccactttgg
     2401 cagcgagagc cactggtggg ggctggtcga actctggcag cagggccggc tcgaggggcg
     2461 ggagatgtgg cgccgtcagg ccgaggtgat gcgcctgacc gagacggagc tgcacgagtt
     2521 tctcgcccct ctggccatcg atacctcctt cggcatgctg gccggctttg cccggcaaga
     2581 gggaataccc gtcaccatcg tcagcgacgg catggatgcc tatatcgccc ccatcctggc
     2641 tgcgcacgga ttcggcgatc ttcccctgcg caccaaccgg atggtgctga cgcccgaggg
     2701 cgctgtccgg gtcgaattcc cctggtccgg ccagggctgc ggacagtgcg ccaactgcaa
     2761 ggggcttcac gtgcgccggg agcggctgcc gggagagacc accgtctaca tcggcgacgg
     2821 cccttccgat atctgcggcg cccgcgaggc cgacatcgtc tttgcaaaaa agcagctcct
     2881 ggcctggtgc agggagaaca gccatccgca ttaccccttc acctctttcg ccgacatcca
     2941 tgaccatttg caggggctgc tcaagcggta aagggaaccg ctgcggcgat ttcttccccc
     3001 aaaaagcatt gacttggacg gggtgatttt gtaagtttcc ctgaggaaag gagattcaag
     3061 tggagataca gttttttggc ggcgcgcgtt cagtgaccgg aaccatgcat gtattgattg
     3121 tacgggggaa aaaaatttta ctggactgcg gactttatca ggggaagcgc gacgaggcct
     3181 ttgagcgcaa ccgccagctc cccttcgatg ccgcgagcat agatgccatg gtgctctccc
     3241 acgcccatat cgaccacagc ggcaacatcc cccagctggt caagctcggg ttcaccggga
     3301 atattgtctg cacccatgcc acccgcgatc tctgcagcat catgctgcag gattcgggat
     3361 acatccagga gaaggacgcc gagtacgtca acaagcggcg ggctcgcaag gggcagaggc
     3421 cggtggagcc cctttacacg gcgcgtgacg ccgtcaaggc gatgaaccag ttcttcagcg
     3481 tcgggtacga acgcaaggtg caaatcgccg acggggtgtc ggtcaccttt catgacgcag
     3541 ggcatatcct cggcgcagcc atctccgccc tggacatcaa tgatgacggc aagtccattc
     3601 gtctggtctt cagcggcgat ctggggcggc cgggccagcc catcctgcgt gacccagcca
     3661 tcctccgtga agcggacatc ctcttcaccg agagcaccta tggcggccgt ttccatgatg
     3721 attttgccac tctgaaccag aagctggcgc aggtggtcaa cactaccgtc aaccgcggcg
     3781 gcaagatcat cgtcccggcc ttttctgtgg gccgcacaca ggagatcgtc tacgccctgc
     3841 atcagctcac cctggcgggt gaaatcccgg agctgccgat cttcgtcgat tctccactct
     3901 cccttaatgc caccgaggtc tttcgcgccc atcccgaatg ctatgacgaa gagacggcgg
     3961 actttctcga gagcaccgac gacgtctttg gctggaatcg tctccgctac atccgtaccg
     4021 ctgaagagtc caaaaagatc aacgagatca aatccccctg tatcatcatc tcggcct
//
LOCUS       DAOPWU010000320         4052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_22332_length_4052_cov_3.987491, whole genome shotgun sequence.
ACCESSION   DAOPWU010000320 DAOPWU010000000
VERSION     DAOPWU010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22332_length_4052_cov_3.987491"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKM23_12915"
     CDS             complement(<1..238)
                     /locus_tag="PKM23_12915"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNY92418.1"
                     /translation="MGTSRTLKAGAALLLLISFTGVGISQTPVVKKRIAVFSFEDKTN
                     HKNRWWSGQSVGEGMADMLITELVKSGRYQVIERQ"
     gene            complement(280..1506)
                     /locus_tag="PKM23_12920"
     CDS             complement(280..1506)
                     /locus_tag="PKM23_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92419.1"
                     /translation="MNRKILLIIGACLLCWGPALPQTAPGSTQVEAVGLGAITYNDIP
                     AARDRALDDAKRKAVEYVLGTVIDAETRVENYQVMEDRILSWTRGYVRTYRILHEGKS
                     GDDIYEVKVLATVDQASMTRDVEAVQNLIQSMGNPRVMFIIDEQNIGATQDRYHYFQV
                     DVTVSETTLMNKFLEKSFQVVDPAVVRQNRERDAVVAVINGDPKAAAAIGATLEAEVV
                     VTGKAMAKVATGINLGGMKSCQASLTARVIDADVGTVIATASKTAAYPHIDELVGGSK
                     AIEKAANQLGDELIAKILDKWQSRVYSANTVKLLVTGINSYNEAANFRGNLGFAARGI
                     KSVNQRNLAASTAEYDVQIQGNADQLARELDGRELKGYTLSVQGVTANKITIRLAHSV
                     KTSAEMEEEPDSSAVE"
     gene            complement(1628..2782)
                     /locus_tag="PKM23_12925"
     CDS             complement(1628..2782)
                     /locus_tag="PKM23_12925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNY92420.1"
                     /translation="MKARLLFYRGAALLLLLLAAGCGPKAIKEASVQDTPEKHFGRGM
                     YELERGNMTSARTEFERAIALNPDYGEAFSGLALISATEGDYKKAHELADKALDKNGK
                     SLESLVVKGRIFAMERKGDDWVEKAAKQFDKALAINPSSAKALFYKGEAYKEGYQFGE
                     AAGLYSKVIALKGDYAAQADQAWERMQKIQRAAPGSKEGARIALIDEIDRADVAVLFM
                     DELKLMEILKQKRAPEYNTGFKAPEDPTAIQTSVTSRSAEVTDIDNHWAKSWISEIIA
                     ARGMELFPDRTFRPDEKITRVDYAMLLQNILIMATGDESLATRYIGQPSRFPDVNPTH
                     YAYNAVCLMVDRGIMKADKMTGAFDLKSHVSGADALLIIRDFQNAMRLAF"
     gene            complement(2816..3739)
                     /locus_tag="PKM23_12930"
     CDS             complement(2816..3739)
                     /locus_tag="PKM23_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92421.1"
                     /translation="MRIARLLSVTVMLIALVAMPCLAQTVTQQVGTAGSIDWSNQILR
                     STGVGSPNPNMPETAQRAGALEAAKRVALRNLLETVKGMSLDSETTVRNFMVENDVIV
                     TRVSGILRNFTVVDTKYMSTGDIEVTIEMPISGALADALLPQPSGASLGPVTPASAQA
                     VCPTCGQPWPAGRPLPSGGTTPAHSAAPAPQGNSGAYTGLVIDAKGLNLRPAMSPKIL
                     DESGEEIYGSKNVSREWAVQIGMVGYDKDVNRARSDERVTDNPLVVKAVKVSGDNKAD
                     VVVAAASAAAIRSAAASQNFLDHCKVMFVVD"
BASE COUNT          759 a         1172 c         1241 g          880 t
ORIGIN      
        1 cctggcgttc gatgacctgg tagcgcccgc tcttgaccag ttcggtgatg agcatgtcgg
       61 ccatcccttc accgacggat tggccgctcc accagcggtt cttgtggttg gtcttgtcct
      121 caaagctgaa aaccgcgatt cgtttcttca cgaccggggt ctgggagatg ccaaccccgg
      181 tgaatgaaat gagcagcagg agagcagctc ctgccttcaa tgttctggat gtacccatac
      241 agccctcctg tttcccttgc tgccgacggc cactactccc tattccaccg cagaggaatc
      301 cggttcttct tccatctccg cgctggtctt gacggaatgg gcgaggcgga tggtgatctt
      361 gttggcggtg acgccctgta ccgagagggt atagcccttg agttcccggc cgtccagctc
      421 acgggcgagc tggtctgcgt tgccctggat ctgcacatca tactcagcgg tggaggcggc
      481 caggttgcgc tggtttaccg acttgatgcc acgggccgcg aagccgaggt tgccgcgaaa
      541 attggcagcc tcattgtaac tgttgatccc ggtcaccagc agtttgacgg tattggcgct
      601 gtagacccgg ctctgccatt tgtcgagtat cttcgcgatc aactcgtcac cgagctggtt
      661 agcggctttt tcgatcgcct tgctccctcc caccagttca tcgatatggg gataggcggc
      721 ggtcttgctg gcagtagcga tgacggtgcc gacatcggca tcgatgaccc gggcggtgag
      781 gctggcctgg caggatttca tgccgccgag attgatgccg gtggccacct tggccatggc
      841 cttgcccgtg accacaacct cagcctcgag ggtggcgccg atggcagcag cggcttttgg
      901 atcgccatta atgacggcga cgaccgcatc ccgctcacgg ttctgtcgca cgaccgcggg
      961 atcgaccacc tgaaaactct tttcaaggaa tttgttcatc agggtggttt cagagaccgt
     1021 gacatcaacc tgaaagtagt gatagcgatc ctgggttgcg ccgatgttct gctcgtcgat
     1081 gatgaacatc accctgggat tgcccatgct ctgaatgagg ttctggaccg cctccacatc
     1141 cctggtcatg ctggcctgat ccaccgtggc gagcaccttc acttcgtaga tgtcatcgcc
     1201 gctcttgccc tcgtgcagga tcctgtaggt gcgcacatag ccccgcgtcc aggagaggat
     1261 gcggtcctcc atcacctggt aattctcgac gcgggtctcg gcatcgatga ctgtgcccag
     1321 gacgtattcc acggccttgc gcttggcgtc gtcgagggcg cgatcccgtg cagccgggat
     1381 gtcattgtag gtgatggccc caagtccgac cgcctcgacc tgtgtggagc cgggtgccgt
     1441 ctgtggaagt gcaggacccc agcagaggag gcaggcaccg ataataagaa ggatttttct
     1501 gttcaaggag tactcctcac tgctgtgggt gagcgccgca gccgggccgc tgccctatga
     1561 gcaaggtgag cgggcaggaa cagggccgct caccttgctc tgaaggtttc aggaaaggca
     1621 tgcccggcta gaaggccagg cgcatggcgt tctggaaatc ccggatgatc agcagggcat
     1681 cagcaccgga gacatgagac ttgagatcga aggcccctgt catcttgtcc gccttcatta
     1741 tgccacggtc taccatcagg cagacggcat tgtaggcata gtgggtggga ttgacatcgg
     1801 ggaagcggga aggctggccg atgtacctgg tcgccagact ttcgtccccg gtggccatga
     1861 tcagaatatt ttgcaggagc atggcataat ccacgcgggt gatcttttcg tccgggcgga
     1921 aggtgcggtc gggaaagagc tccatgccgc gggcggcgat gatttcgctg atccagcttt
     1981 tggcccagtg gttgtcaata tccgtcacct cagcggaacg ggaggtgacg ctggtctgta
     2041 tggcagtggg atcctccggg gccttgaagc cggtgttgta ctcgggtgcc cgtttctgct
     2101 tcagaatctc cataagcttc aattcatcca taaagagcac ggcgacatcg gcgcggtcga
     2161 tttcgtcgat gagggcgatt ctggcaccct ccttgctgcc cggtgcggcg cgctggatct
     2221 tttgcatccg ttcccaggcc tggtccgcct gggcggcata gtcgcccttg agggcgatca
     2281 ccttgctgta gaggccggct gcttcgccga actggtaccc ctccttgtag gcttcaccct
     2341 tgtagaagag ggccttggct gaggagggat tgatggcgag ggccttgtcg aactgtttcg
     2401 ccgccttttc aacccagtcg tcgcccttgc gttccatggc gaagatgcgg ccctttacga
     2461 ccagagattc caacgacttg ccgttcttgt cgagcgcctt gtcggcgagc tcatgggcct
     2521 ttttataatc cccctcggtg gcggaaatca aggccagccc ggagaaggct tcgccgtagt
     2581 caggattgag ggcgatggcg cgctcgaatt ccgtccgggc tgaggtcatg ttgccccgct
     2641 caagttcata catcccgcgg ccgaaatgct tctcgggggt atcctggacg gaggcctcct
     2701 tgatggcttt gggaccgcat ccagcggcga gaaggagcag aagaagggct gcgccccggt
     2761 aaaagagcaa tcttgctttc atcatattct ccacattcaa tgggctcagg ctgatctagt
     2821 cgacgacaaa catcaccttg cagtgatcga ggaaattctg cgaggcggct gcgctgcgga
     2881 tggcagcggc cgaggcagcg gcgacgacga catcggcctt gttgtctccg gagaccttga
     2941 cagccttgac gacgagggga ttatcggtca ccctttcgtc gctgcgggcg cggttgacat
     3001 ccttgtcata gccgaccatg ccgatctgaa ccgcccattc ccggctgacg tttttggagc
     3061 cgtaaatctc ctcccccgat tcgtcgagga tcttggggga catggcgggg cgaagattca
     3121 gccccttggc gtcgataacc agaccggtat aggccccgct gttcccctgg ggagccgggg
     3181 cggcgctgtg cgcgggggtg gtcccgccgg agggcagagg ccgtccggct ggccagggct
     3241 ggccgcaggt cggacaaaca gcctgggcag aggctggggt gaccggaccg aggctcgcac
     3301 ctgagggctg cggcagcagg gcatcagcaa gcgcgccgga aatgggcatt tcgatggtca
     3361 cttcgatgtc gcctgtcgac atgtacttgg tatcgacgac agtgaaattg cgcagtatgc
     3421 ccgatacacg ggtgacgatg acatcgttct caaccatgaa attgcgcacc gtggtttccg
     3481 agtcaaggct catccccttg accgtctcga gcagattgcg caaggcgaca cgtttcgccg
     3541 cctcgagggc gcctgcgcgc tgggccgttt caggcatgtt cggattggga gaaccaacgc
     3601 cggtggagcg caggatctga ttggaccaat caatgctccc ggccgtgccg acctgttggg
     3661 tgacggtctg ggcgaggcag ggcatcgcaa ccagggcgat tagcatgacg gtcaccgaca
     3721 gcaaacgagc gattctcatt acatcctcct cgacgattcg ggttatgaat acagcatagt
     3781 ttacagcgct gcggtatctt gtcgggtgat agcggtcaca aaaggctgga ttacaccatc
     3841 caccaccact gccggactca gccggattcc accccaaaga aagagaggcc gaaactgatc
     3901 cggcctcgca atagttgata taacttaaca aagaaacaag agattgtcaa gcccaaattg
     3961 caggagggta cccccgaaat ctgggtccca cctgactcct ggcacagcgt accccaatcc
     4021 caccgtacga tttccagcat gttctcggcc tg
//
LOCUS       DAOPWU010000321         4044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_22389_length_4044_cov_4.314866, whole genome shotgun sequence.
ACCESSION   DAOPWU010000321 DAOPWU010000000
VERSION     DAOPWU010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22389_length_4044_cov_4.314866"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PKM23_12935"
     CDS             <1..898
                     /locus_tag="PKM23_12935"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNY92422.1"
                     /translation="RQAAAASTPGQARVVLAAAGVDPRQPDLKVLGSALDRGLEALFG
                     RPAPRVWASLFSPRDVVGLKVNCLAGRGISTSPILVELVVDRLRTAGVNPGQIIIWDR
                     LNADLKRGGFPIITEAGRVRCFGNDVAGYSRRLFESGSVCSRLSNLVAETCTALINLP
                     VLKDHGIVGLSAAMKNWFGAIDNPNKYHDHCGDPFVAETCSLPLLRDKIRLTICDAIT
                     AQYEGGPPCMPQWCWPLQRLILATDMVALDTVAWQIIEDKRRQAGMASLREAGREPAY
                     IRTAAGLGLGIADPVRIEIVKE"
     gene            972..2087
                     /gene="amrS"
                     /locus_tag="PKM23_12940"
     CDS             972..2087
                     /gene="amrS"
                     /locus_tag="PKM23_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNY92423.1"
                     /translation="MITRRCFLASAACAAPLLSVAGRSVFPASVTVEKGGTVEARYYE
                     KLPNRKIRCNLCPRSCVIDDLERGYCGVRENRGGTYYTLVYGHPCSLYVDPIEKKPLF
                     HFLPASRALSLGTAGCNVFCKFCQNWEISQSRPEQLEAMEIPPERMVEMARRENCPAI
                     AYTYNEPVVFAEYMHDIALSARRQGIRTVMISNGYIQKKPMLDLCRSLSAVKIDLKAF
                     SERFYKELVSGELRPVLDTLVLLRGEQIWFEIVYLVIPGENDSRSELTSLCRWIAAEL
                     GMDVPIHFTRFYPQYRMTNLPPTPVKSLQEARKIGLEAGLNFVYTGNLPGDEGENTWC
                     PACHALLIRRLGYTVVENRIKSSLCPECKTTIPGVWK"
     gene            2167..3624
                     /gene="amrB"
                     /locus_tag="PKM23_12945"
     CDS             2167..3624
                     /gene="amrB"
                     /locus_tag="PKM23_12945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNY92424.1"
                     /translation="MAALLFLYSPPCKASAGEIRKAAVAGQFYPVEAEKLHAELARYL
                     EKAPVSSVAGEILGISVPHAGYAYSARTAASAYKAVSGRSYDLIIILAPSHQDYFTGA
                     TIYPGEAYETPLGRIRIDHDAAVRLNRTSSLFRLSGLGHGSREHSLEVQLPYVQYLFP
                     GTPLLPIMVGECDWNSCQKMGKALASLSAGRRVLIIASTDLYHGYSYQECLASDAATL
                     AAFTRMDPQALHAGLASERYRACGGAAVVIMEVAVLARGRCAATILARTNSGDVTGDK
                     SGYIVGYASGLYYLQEKAMNKKQFSPLPIDAQIELLRMARESIKHYLKHGVVPEFRSD
                     IPILQEKRGVFVTITKNGSLRGCIGMHEADKPLYQAVPDRAVAAAFEDPRFMPLQLEE
                     LERIKIKVSVYLTNVYEAESLDDFVMGKHGIILIKGSRGATYLPEVPVEAGWKSKEEE
                     MVSLCQKAGLPPDGWREGAQILLYETQVFDDSLLR"
     gene            complement(3740..>4044)
                     /locus_tag="PKM23_12950"
     CDS             complement(3740..>4044)
                     /locus_tag="PKM23_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92425.1"
                     /translation="ALRRLELILNVLNEITPHFQGGMDPIFPRLKTEELEMLPGTLDR
                     LRPIKRNMAGEGRKNARRGEDEDEQAHQCHGGEQQPVITFFFSMHGYENNPWFMEQ"
BASE COUNT          823 a         1271 c         1103 g          847 t
ORIGIN      
        1 ccgccaggcc gctgcagcct caaccccggg ccaggcccgc gtcgttttgg ccgccgccgg
       61 ggtcgacccg cgccagcccg acctcaaggt cctcggcagc gccctggacc gcggcctcga
      121 agcccttttc ggccggccag ccccccgggt ctgggccagc ctcttctctc cccgcgatgt
      181 ggtcgggctt aaggtcaact gcctcgctgg acgcggcatc tcgaccagcc ccattctggt
      241 ggagctggtg gtcgaccgcc tgcggacggc cggcgtgaac ccgggtcaaa tcattatctg
      301 ggaccgcctt aacgctgatc tcaagcgcgg aggtttcccc atcatcaccg aggccggacg
      361 cgtgcgctgt tttggcaacg atgtcgcggg ctattcgcgc cgcctcttcg aatcaggatc
      421 ggtgtgcagc cgcctctcca atctcgttgc tgaaacctgc accgccctga tcaatctgcc
      481 ggtgttaaaa gaccacggca tcgtcggcct cagcgctgcc atgaagaact ggttcggtgc
      541 cattgacaat cccaataaat accacgatca ctgcggcgat ccctttgtgg ctgaaacctg
      601 cagcctgccc cttctccgcg ataaaatccg gctgacgata tgcgatgcca tcacggccca
      661 gtacgaaggt ggaccgccct gtatgccgca gtggtgctgg cctctccagc gcttgatcct
      721 ggcgacggat atggtcgccc tcgacaccgt cgcatggcag atcatcgagg ataagcgccg
      781 acaggcaggc atggcctcac tgcgcgaagc cggccgggaa cccgcctaca tccggacggc
      841 tgcgggcttg gggctcggca tcgctgaccc cgtgcggatc gagattgtaa aggaatgaag
      901 gggagagacc tctttccggc gctgctccca atcctgtacc agaccgggag agaggcgcga
      961 cggaggaaat aatgatcaca cgcagatgct ttctcgcttc ggccgcctgc gccgcccctc
     1021 ttctgtcagt tgctggccga tccgtatttc ccgcttccgt cactgtggaa aaaggtggga
     1081 cagtcgaggc gcgctattac gaaaaattac ccaaccgcaa gatccgctgc aacctctgcc
     1141 cccgcagctg cgtcatcgac gatctcgaac gtggttattg cggggtgagg gagaaccgcg
     1201 ggggcaccta ttataccctg gtctacggac atccctgctc actctatgtc gatcccatcg
     1261 aaaaaaaacc gctctttcat tttctccccg cgagccgagc cctctccctg ggcacagccg
     1321 gctgtaatgt cttttgcaag ttctgccaga attgggaaat ctcgcagtcc cgcccggaac
     1381 aactggaggc catggaaatc ccgcctgagc gcatggtgga gatggcgcgg cgggagaact
     1441 gccccgccat tgcctacacc tacaacgagc cagtcgtctt tgccgagtat atgcacgata
     1501 tcgccttgag cgcgcggcgc cagggcatcc gtacagtgat gatttccaac ggctatatcc
     1561 agaaaaagcc tatgcttgac ctctgcagga gcctctccgc cgtcaagatc gatctcaagg
     1621 ccttcagcga gcgcttttat aaggaactgg tctccggaga actgcgtccg gtgctcgaca
     1681 ccctcgttct gctgcgcggg gaacaaatct ggttcgaaat cgtatacctc gtcatcccgg
     1741 gtgagaatga cagccgttcc gagctgacct ccctgtgcag gtggatcgct gcagaactgg
     1801 ggatggatgt gcccatccac ttcacccgtt tttatcccca gtaccggatg accaatctgc
     1861 cgccgacacc ggtcaaaagc ctgcaggaag cgcgcaagat cggccttgag gcggggctga
     1921 atttcgtcta tacggggaat cttcccggtg atgagggaga aaatacctgg tgccccgcct
     1981 gccatgccct gctcattcgc cggctcggct acacggtcgt cgaaaaccgg atcaaatcct
     2041 ccctatgccc ggagtgcaag accacgatcc ctggagtctg gaaatgataa tcacgtctgt
     2101 tgaatggtct taaatgcaga aatcggaggg atgtgcgtga tccttaaagc accacttcag
     2161 gtactgttgg ccgctttgct ttttttatat tcgcccccat gcaaagcatc agccggagag
     2221 atccggaaag cagccgttgc cgggcagttt tatcccgttg aggcggaaaa gctgcacgcc
     2281 gaacttgcac gctacctgga aaaggccccg gtctcctctg ttgcgggtga aatactcggc
     2341 atcagcgtcc cccatgccgg ctatgcctat tcagcccgca cggctgcctc cgcctacaag
     2401 gcggtctccg gccggtccta cgacctcatc atcattctcg caccgagcca ccaggactat
     2461 ttcaccggcg ccaccatcta tccaggcgag gcctacgaaa ccccgctcgg ccgcatcaga
     2521 atcgatcatg acgccgccgt gcggctcaat cgcaccagca gccttttccg tctctccggg
     2581 ctagggcatg gctcgcgaga acacagcctc gaggttcaac tcccctacgt gcagtacctt
     2641 ttccccggaa ccccgctgtt gcccatcatg gtcggagaat gcgactggaa ctcctgtcaa
     2701 aagatgggaa aggccctcgc aagcctctcg gcagggcgcc gggtactcat catcgccagc
     2761 accgacctct atcacggcta ttcctatcag gagtgcctcg caagcgacgc agccaccctc
     2821 gctgccttta cccgcatgga cccgcaggcc ctccatgccg gacttgcctc cgaacggtat
     2881 cgcgcctgcg ggggcgctgc cgtcgtcatc atggaggtcg ccgtactagc acgtggacgc
     2941 tgtgccgcca ccatcctcgc ccgcaccaac tccggcgacg taaccggcga taagagcggc
     3001 tatattgtgg gttacgcctc gggactctat tatctgcagg agaaagcgat gaataaaaag
     3061 caattttcac ctctgcccat cgatgcacag atcgaattat tgcgcatggc cagggaaagc
     3121 atcaagcatt acttgaagca tggtgttgtt ccggaattcc gcagcgacat ccccatcctc
     3181 caggagaaga ggggggtttt tgtaaccatc actaagaacg gatcgctgcg cggctgtatt
     3241 ggcatgcacg aagccgacaa gccgctctat caggcggtcc ccgaccgtgc agtggccgcc
     3301 gcttttgaag atccccgctt tatgcccctg cagctggagg aactcgagag gatcaaaatc
     3361 aaggtctcgg tctatctgac caatgtttac gaggccgaaa gtctcgatga tttcgtcatg
     3421 ggcaaacatg gcatcattct catcaagggc agtcggggag caacctatct gcccgaggtg
     3481 cctgttgagg ccggttggaa gagcaaggag gaggaaatgg tcagtctctg ccagaaagcg
     3541 ggcctgcccc cggatggctg gcgggagggc gcacagatcc ttctctacga gacccaggtc
     3601 ttcgatgaca gtttgctccg ttaaccggct gcccctctca gaagggtatt gctcgcccgt
     3661 gaaagctgca cctcaaagcg ctgatatttc ccttgatttg aagcttagaa ttagttaatt
     3721 ttcaaaaatt taatcattct cactgttcca tgaaccaggg attgttctca tacccatgca
     3781 tggagaaaaa aaaggttatc actggctgct gctcgccgcc gtggcattga tgggcttgct
     3841 catcttcgtc ctcaccgcgc cgcgcatttt ttctcccttc ccccgccata tttcgcttaa
     3901 tcgggcggag gcgatcaaga gtgccgggca gcatctccag ctcttcggtt ttcagccggg
     3961 ggaatatcgg gtccattcca ccctggaagt gcggcgtgat ctcattcaat acattgagaa
     4021 tcagttcaag cctgcggagc gcga
//
LOCUS       DAOPWU010000322         4023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_22545_length_4023_cov_4.262349, whole genome shotgun sequence.
ACCESSION   DAOPWU010000322 DAOPWU010000000
VERSION     DAOPWU010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22545_length_4023_cov_4.262349"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..2424)
                     /locus_tag="PKM23_12955"
     CDS             complement(184..2424)
                     /locus_tag="PKM23_12955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY92426.1"
                     /translation="MHSTRNLLLLLPLFCSLFFSGCAGSRKQEDEVDAFLRSSAAYYI
                     KKGSDAIEAGRYLEAVPHFRQAIALTPFDPVAHNDLGVAFYHLGQLDSALVYYQAAIR
                     LRPDYIRAISNLSKTYADLGDKNYALAAAERIIELAPSATAGYLLQAEIYDKHEQYDE
                     AIQATLTALAIDSTQQDLRNNLGVLYFHSGRLDLAIDCYQQVIDMDSTYAVAYFNLGN
                     VLARKCLLEEAQHQYRRALHFRPEMTSAANNHGLVHLFQGRLEEAGSDFYRALASDST
                     APAALYNLSIVQMRLDSLERALASIDRAIAHKPAVANFYLQEGSVLQRLGREEEALIA
                     MQKAVALDSTLSAGYNSLGNLIVSEHPDLATEAYEKAAANYDEYMQRRYGRSTRIMEK
                     GYFDLMATCKDRWQIRTDHAMIYNNLGKSYLRLGSYEQAGKAFTRAPELQPDLWEPAE
                     NLAVVLLAQNREKEARAWLAAGRLNRARAALLADSLDAAENMLSEAGQLQPGLQGSHE
                     VLARLYERRGETAKAESTLRNGVRLYPADAGVHQAYGRFLSRQGRFSEAREQLLKAVA
                     LDPKRDEVHYQLSTIYRTLGETQKADEEVARSHHVLGEGYEEAGFFDRAMEEYQLAAA
                     ADPARMEYVASQGIIHLKKGLYVDAERLFRAVLEKEEKNPRALYGMGVLQGERKQHEQ
                     AVAWLQSAIAVHPDNGQFHQALAVNYYFLGRLDSARAEALAAQKLGVTLHENFLKAID
                     LPTPLH"
     gene            complement(2491..3360)
                     /locus_tag="PKM23_12960"
     CDS             complement(2491..3360)
                     /locus_tag="PKM23_12960"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNY92427.1"
                     /translation="MLKGIWISLRPKQWIKNIIVFAGLFFAEDFLVLSKILDTVAAFI
                     LFCMASSSGYLVNDVIDRRKDALHPRKRLRPIASGAVAPRLAVTLALLLMAAGLVGAW
                     LLNHEFALILAVYLAFTLSYSLFLKRVIILDVLIIASGFVLRAIGGTIAARESVSSWL
                     IICTIFLSLFLALTKRRSEVKTLGEKAAEVRTTLALYSVELLDQMINIVTAACLMAYA
                     LYTLDAGTVDKFATRNLAFTLPFVIYGLFRYLYLVLHLNIGETPETVLTHDRPILICI
                     LAYILTVASILYF"
     gene            complement(3454..>4023)
                     /locus_tag="PKM23_12965"
     CDS             complement(3454..>4023)
                     /locus_tag="PKM23_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92428.1"
                     /translation="PLQVLSYTPADTLVVLELDTPCNFDVVAIDPDGDSLRYQWAFNG
                     MPVGSQQSLKVTANPAFPARSRVTVRIYTLRSSETLSWTLDIHTGVEAEVSEPEAFVL
                     GQNFPNPFNPVTHIPFRAGRNAEVSVVIYNASGQLVRRLHDGFTAGGWHQVIWDARDE
                     AGQPVPSGTYYCRMNSGPFQQIKKLLLLK"
BASE COUNT          806 a         1123 c         1339 g          755 t
ORIGIN      
        1 atagaccctt acgcggggca cgtcacagcg tttctgcagt ctggccagga gcgtacgggc
       61 gctctgctcc agccctgaat accccggtgc ggtccccggg ccgcaccccc cccctccggc
      121 acccggaaat catccatttt ttacaccagc tgacgcagcc gtatggccgc ctcctcccgc
      181 acctcaatgc aagggtgttg gcagatcgat cgccttgaga aaattttcat gcagggtcac
      241 acccagtttt tgtgcggcca gggcctcggc gcgggcggaa tccagccggc cgaggaagta
      301 atagttgaca gccagggcct gatggaactg gccgttgtcc ggatggacag cgatggcgga
      361 ttgcagccag gccaccgcct gttcatgctg cttgcgttcg ccctgcagca cccccatgcc
      421 gtatagagcc cggggattct tttcctcctt ctcgaggacg gcgcgaaaga gacgctcggc
      481 atccacatat agcccctttt tcagatggat aatcccctga ctggcgacat actccatccg
      541 ggcgggatcg gccgcggcgg cgagctggta ttcctccatg gcacggtcga aaaagcccgc
      601 ctcctcatag ccctcaccga gaacatgatg gctgcgggcc acctcctcgt cggccttctg
      661 ggtctcgccc agggtacggt agatggtcga gagctggtaa tggacctcgt cccgtttggg
      721 atcgagggcg acggccttga gcagctgctc tctggcctcg ctaaagcgtc cctggcggct
      781 gaggaagcgg ccgtaggcct ggtggactcc cgcatccgcg ggatagagtc tgactccgtt
      841 cctgagggtg ctctccgcct tggcagtttc gccccggcgt tcgtagagcc gggcgagcac
      901 ctcatggctg ccctgcaatc cgggctgcag ctgacccgcc tcgctgagca tgttctcggc
      961 ggcatcgagg gaatcggcga gcagggcggc acgggcgcgg ttgagccttc cggccgccag
     1021 ccaggcacgg gcctctttct cccggttttg tgcgagcagg accacagcca ggttttcggc
     1081 gggttcccag agatcgggct gcagctccgg ggccctggtg aaggccttgc ccgcttgctc
     1141 gtagctgccg agtcgcagat aggatttgcc cagattattg tagatcatgg catgatcggt
     1201 gcgaatctgc cagcgatcct tgcaggtagc catcaggtca aaatagccct tttccatgat
     1261 ccgggtggag cggccgtacc gcctttgcat gtactcgtca tagttggccg ccgccttttc
     1321 ataggcttcg gtggcgaggt cggggtgctc cgaaacgatg aggttgccga ggctgttata
     1381 gccggcggag agggttgaat cgagcgcgac cgccttttgc atggcgatga gggcctcctc
     1441 ctcccgtccg aggcgctgga ggacgctgcc ctcctgaagg taaaagttgg cgacggcggg
     1501 cttgtgcgcg atcgcccgat cgatcgaggc gagggcccgt tcaagggagt cgagacgcat
     1561 ctgtacgatc gagaggttgt agagtgcggc tggggcagtg gaatcggagg ccagcgcccg
     1621 gtaaaagtcg cttcccgcct cctccagacg gccctggaag agatgcacga gaccgtgatt
     1681 attggccgcg gaggtcatct ccggcctgaa atgcagggcc cggcggtatt ggtgctgggc
     1741 ctcctcgagc aggcatttgc gcgccaggac gttgccgaga ttaaaatagg cgacggcata
     1801 ggttgaatcc atgtcgatca cctgctggta gcagtcgatg gccaggtcga gccggccgct
     1861 gtggaaatag agcacaccca gattattgcg caggtcctgc tgggtggaat cgatggccag
     1921 ggcggtgagg gtggcctgga tggcttcatc gtactgctca tgtttgtcat agatctccgc
     1981 ttgcagcaaa tagcccgcag tggcggaagg agccagctca atgatacgct cggctgcggc
     2041 gagggcgtaa ttcttgtccc cgagatcggc ataggtcttg gagagattac tgatcgcccg
     2101 gatgtaatcg ggccgcaggc ggatggccgc ctggtagtaa accagggcgg aatcgagctg
     2161 gccgaggtga taaaaggcca cgccaaggtc gttgtgcgcc accggatcaa agggagtgag
     2221 cgcaatcgcc tgccggaaat ggggcaccgc ctcgagatag cgccccgcct cgatggcatc
     2281 cgagcccttt ttgatataat aggcggcgga actgcgcaga aaggcatcca cctcatcctc
     2341 ttgcttgcgg ctgcccgcgc agccggagaa aaaaagggag caaaagaggg gcaacaacag
     2401 cagcaggttc ctggtcgaat gcatagggat gcctcctgtc agggagaata aaatggttgt
     2461 tgacgaggcc ggtgggagaa aggggatcta tcagaaatag aggatcgagg ccaccgtcag
     2521 aatataggcg aggatacaga tcaggatcgg ccggtcatgg gtcaaaaccg tctcgggggt
     2581 ctcgccgatg ttgagatgca ggaccagata gaggtagcgg aagaggccgt agatgacgaa
     2641 cgggagtgtg aaggccagat tgcgggtggc gaatttgtcg acagtgcccg cgtcgagggt
     2701 gtaaagggca taggccatca gacaggccgc ggtgacgatg ttgatcatct ggtcgagcag
     2761 ttcgacgctg tagagggcga gagtcgtgcg gacctcggcc gccttttccc ccagggtctt
     2821 cacttcgctg cggcgtttgg tcagggcgag aaagagggag aggaagatcg tgcagatgat
     2881 cagccacgat gagacgctct cccgggctgc gatcgttccg ccgatggcgc gcagcacgaa
     2941 tcccgaggcg atgatcagca catccagaat gatcacgcgc ttgagaaaga gcgaatagga
     3001 gagggtgaaa gccaggtaaa cggccaggat cagggcgaac tcatggttga gcagccaggc
     3061 tccaaccagc cccgcggcca tgagcaagag cgccagggtg acggccaggc gcggtgcgac
     3121 ggcgccggaa gcgatggggc gcagccgttt gcggggatgg agggcatcct tcctgcggtc
     3181 gatgacgtcg ttgacgagat aaccgctgct ggaggccata cagaagagga taaaggcggc
     3241 gacggtatcg aggatcttgc tgaggacgag aaaatcttcg gcgaaaaaaa gaccggcgaa
     3301 gacgatgata tttttaatcc actgtttggg gcgcagcgaa atccagatac ccttcagcat
     3361 atctcctcca tcccttcccc gagcgcgctg cagccgccgg ggccatacct tgcagcaggc
     3421 cccgggacgg tgcatggagg aaagatagca cttttatttt aacaataaaa gcttttttat
     3481 ctgctggaag gggccgctgt tcatgcggca gtaataggtg ccggagggca cgggctggcc
     3541 ggcctcgtcg cgggcgtccc agatcacctg gtgccagccg ccagccgtaa agccatcatg
     3601 gagccggcgc acgagctgac ctgaggcgtt gtagatgacg acactgacct cagcgttccg
     3661 gccggcccgg aaggggatgt gggtgaccgg attgaaggga ttgggaaagt tctgaccgag
     3721 gacgaaggcc tcgggttcgc tcacctcggc ctccacaccc gtgtggatgt caagggtcca
     3781 ggagagggtt tcgctcgaac gcagggtata aatgcgcacc gtgacccggc tgcgcgccgg
     3841 aaaggcggga ttggccgtaa ccttgaggct ttgttggctt ccgaccggca tgccgttgaa
     3901 cgcccactgg tagcgcaaag agtcgccatc cgggtcgatc gccacaacat caaagttgca
     3961 gggcgtgtcg agctccagga ccaccagggt atcggccggg gtgtagctga gaacctgcag
     4021 ggg
//
LOCUS       DAOPWU010000323         3943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_23208_length_3943_cov_4.962449, whole genome shotgun sequence.
ACCESSION   DAOPWU010000323 DAOPWU010000000
VERSION     DAOPWU010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23208_length_3943_cov_4.962449"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="PKM23_12970"
     CDS             <1..139
                     /locus_tag="PKM23_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018117610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNY92429.1"
                     /translation="TPFCITVDTETLEHETVTVRDRDTMQQERVTIAELTAFLHKKIN
                     G"
     gene            153..968
                     /gene="pyrF"
                     /locus_tag="PKM23_12975"
     CDS             153..968
                     /gene="pyrF"
                     /locus_tag="PKM23_12975"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNY92430.1"
                     /translation="MRFADRLDRVLEEKESLLCVGLDTVLEKIPASLRGEPDPLFAFN
                     RAIIDATLPYAAAYKVNTAFYEAYGLAGWRALEETFRYLPADVIRIADAKRGDIGNTA
                     MMYARGLFAGLGADAVTVNPLLGGDSVMPFLEEESLGVFFLCLTSNPGSRDFQHIQTG
                     EGRLYEVIAHRVRSWNEKGNCGLVVGATHPEQLGAIRTLAPELPLLIPGIGAQGGELE
                     ASVRAGTDKAGRGALYNSSRAILYASAGEDFREAAASAARETRDGLQRAGKRS"
     gene            965..1813
                     /locus_tag="PKM23_12980"
     CDS             965..1813
                     /locus_tag="PKM23_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNY92431.1"
                     /translation="MSPRLHSASAALLLLLLLTGCGQKEDKGNPTGPETPAAVPSNLP
                     AVADSLKWLMVWNDEFNGKSLDGAKWSHVSWVPAGEDQPRRDGFWRPDAAVLNGDGQL
                     MMLTYYDPVKKRYIDGAIHTLGKYEKLYGFFEARIQLQKQPGHWSAFWLFNAQVHTVG
                     DQGRDGTEVDIMEKISPKDDAVYHTLHWDGYEAAHQSVYKLPVIPGLREGWHTYAVWW
                     SESGYRFYIDGTETWRTSAGGVCRNPLHILLSDEVGTWAGSISSAALPDLWLVDFVRV
                     YDLVKK"
     gene            1866..2438
                     /gene="pyrE"
                     /locus_tag="PKM23_12985"
     CDS             1866..2438
                     /gene="pyrE"
                     /locus_tag="PKM23_12985"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNY92432.1"
                     /translation="MTNEEALTIFRESGALLEGHFRLTSGLHSPNYFQCARVLQYPRH
                     AELFCRLIADRFRSEGIEVVISPAIGGIVVGQEVARLLGCRAIFAEREEGMMTLRRGF
                     EIKAGERCLAVEDVVTTGGSVKEVIALAQSAGARVMGAAFIVDRSSGRAQFDVPFFAA
                     LSMEVVTHQPEACPLCAQGIPVVKPGSRKI"
     gene            complement(2520..3383)
                     /locus_tag="PKM23_12990"
     CDS             complement(2520..3383)
                     /locus_tag="PKM23_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNY92433.1"
                     /translation="MTKKKKKKPAETRTKESIEKYLEEIGGFSPLPPEEEIALARRIR
                     KGDEEALDKLVKANLRFVISVAKEYQGQGLPLQDLISEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRVVRLPLNRVGAINKVGRALEALEKEYGREPSMDEIAT
                     RMDMSSFEVADVLKTSARHLSLDEPFKEDEGNSLLDVLESDRYEPPDGTLMRSSLREE
                     IEKVLSTLKPREAEIVKLYFGLDGDRPLTLEEIGEYFTLTRERVRQIKEKALRRLRHR
                     SRLEPLRKYLG"
     gene            complement(3470..>3943)
                     /locus_tag="PKM23_12995"
     CDS             complement(3470..>3943)
                     /locus_tag="PKM23_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNY92434.1"
                     /translation="RLFDSISVCLSKGLGAPVGSVLAGSADFIDRAHRFRKMWGGGMR
                     QAGIIAAAGLYALDHHIDRLKVDHLHARALAHSFLEFPEIEVDLAATQTNIVIVELRK
                     TGLKAHDLVVKLKEKGVLCLATAPTRVRLVTHLDIHDEDTKSACRIIEEILQHSL"
BASE COUNT          738 a         1165 c         1254 g          786 t
ORIGIN      
        1 cacccccttc tgcatcaccg ttgacaccga gaccctcgag cacgagaccg tcaccgtacg
       61 cgaccgcgac accatgcagc aggagcgggt gacgatcgcc gagctgaccg cgttcctgca
      121 caaaaagatc aacggctaga aaggacgaga gcatgcgttt tgctgatcgg ctggaccggg
      181 tgctcgagga gaaggagagc ctgctctgtg tggggctgga tacagtgctc gaaaagattc
      241 cggcctccct gcgcggagag ccagatcccc tcttcgcctt caaccgggcg atcatcgatg
      301 ccacgcttcc ctatgccgcg gcgtacaagg tcaataccgc cttttatgag gcctacgggc
      361 tggcgggatg gcgcgccctc gaggagacgt ttcgctatct gcccgccgat gtgatcagga
      421 tcgccgacgc caagcgcggc gacattggca acaccgccat gatgtacgct cgcggactct
      481 ttgcggggct gggagccgat gcggtgacgg tcaatcccct gctcggcggc gactcggtca
      541 tgccctttct tgaggaggag tccctcgggg tcttttttct ctgtctgacc tccaaccccg
      601 gcagccggga ttttcagcac atccagaccg gcgagggtcg cctctacgag gtgatcgccc
      661 accgggtacg gagctggaac gaaaagggca actgcggcct ggtggtcggc gccacccatc
      721 cggagcagct gggggcgatc cgcaccctgg cccctgagct gcccctgctc atccccggca
      781 tcggcgccca gggcggagaa ctggaggcct cggtgcgggc gggcaccgac aaggcgggcc
      841 ggggcgccct ctataactcg agccgggcca tcctttacgc ctcggccgga gaggatttcc
      901 gcgaggcggc cgccagcgcg gcccgcgaga ccagggatgg actgcaacgg gcgggaaaga
      961 ggtcatgagc cctcggctcc acagcgcctc cgccgcgctg ctgcttcttc tcctcctgac
     1021 cggatgcggc cagaaggaag acaaaggcaa tccgaccggg ccggagaccc cggccgcggt
     1081 gccctccaat ctgccggccg tcgccgactc gctcaaatgg ctgatggtct ggaacgacga
     1141 attcaacggc aagagcctgg atggggccaa atggagccat gtctcctggg tgcccgccgg
     1201 cgaggatcag ccgcgccggg acggcttctg gcgccccgat gccgcggtgc tcaacggaga
     1261 tggccagctg atgatgctca cctactacga cccggtcaag aagcgttaca tcgacggggc
     1321 catccacacc ctgggcaagt acgaaaagct ctatggtttt tttgaagccc gcatccagct
     1381 gcagaaacaa cccggccact ggtccgcctt ctggctcttc aacgctcaag tccataccgt
     1441 gggcgaccag ggccgggatg gcaccgaggt cgatatcatg gaaaagatct cgcccaagga
     1501 cgatgcggtc taccacaccc tgcattggga cggctatgag gctgcgcacc agtcggtcta
     1561 caagctgccg gtgatccccg gcctgcgcga gggctggcat acctacgcgg tctggtggtc
     1621 ggagagcggc taccgttttt atatcgatgg cacagagacc tggcgcacca gcgcgggcgg
     1681 ggtgtgcaga aatcccctcc acatcctgct ctccgatgag gttggcacct gggcgggaag
     1741 catcagttca gccgcgctgc cggatttgtg gctggtcgat ttcgtgcgag tttatgatct
     1801 ggtcaaaaaa tagcatgcgg aataagccat cccctggcgt gagaacgagg agagagaaga
     1861 tcacgatgac caacgaagag gcattgacga tattcaggga aagcggcgcc cttctggagg
     1921 gccattttcg cctcacttct gggctgcaca gccccaacta tttccagtgc gctcgggtgc
     1981 tgcagtatcc ccggcacgcc gagctttttt gccggctgat cgccgaccgc ttccgcagtg
     2041 agggcatcga ggtggtgatc tctccggcca tcggcggcat tgtcgtcggc caggaggtgg
     2101 cgcgcctgct cggatgccgg gcgatctttg ccgaacgcga agaggggatg atgaccctgc
     2161 gccgcggctt tgagatcaag gcgggggaga gatgcctggc cgtcgaggat gtggtcacca
     2221 ccggcggctc ggtcaaggag gtgatcgccc tggcccagtc agccggcgcc cgggtgatgg
     2281 gggcggcttt tatcgtcgac cgcagctccg gcagggcgca gttcgacgtc ccctttttcg
     2341 ccgccctcag catggaggtg gttacccatc agcccgaggc ctgtcccctt tgcgcacaag
     2401 gcatcccggt ggtcaagccg ggcagccgca agatctagat cttttcacaa cctcttcgac
     2461 ctctctcccc cgggagaggc ataaaaaaag ccctgtctga tgacagggct ttggtttttt
     2521 cagccgaggt atttgcgcag cggctccaat cgcgagcggt gtcgcaggcg gcgcagggcc
     2581 ttctccttga tttgccggac gcgttcgcgc gtcagggtga aatactcacc gatctcttcc
     2641 agagtcagag gacggtcgcc atccagccca aaatagagtt tgactatttc cgcttcgcgc
     2701 ggtttgaggg tggagaggac cttttcgatc tcttcgcgga gagaggaacg catcagggtt
     2761 ccatcgggcg gctcatagcg gtcgctttcg agtacgtcaa ggaggctgtt gccctcgtct
     2821 tccttgaagg gttcgtcgag ggagaggtgg cgcgcggagg tcttgaggac atccgccact
     2881 tcaaaggaac tcatgtccat gcgggtggcg atctcatcca tgctgggttc gcggccgtac
     2941 tccttttcga gggcttcgag cgcgcggccc accttgttga tcgcccccac gcggttgagg
     3001 gggagacgca ccacacggga ttgttcagcc agtgcctgca ggatcgattg acgaatccac
     3061 cagacagcgt aggagatgaa cttgaagcct ctggtttcat cgaaacgctg ggcagccttg
     3121 atgagaccga gattgccttc gctgatcaga tcctggaggg gcaatccctg gccctggtat
     3181 tccttggcga cgctgatgac aaagcgcaga ttggccttga cgagcttgtc cagagcttct
     3241 tcgtcaccct tgcgaatacg ccgggccagt gcgatctctt cttccggggg aaggggagag
     3301 aatccgccaa tctcctccag atacttttca atcgactcct tggtcctggt ttcggctggt
     3361 tttttctttt tctttttggt cattaactgc acttgcgttg gcataggtgg aaacgggcct
     3421 tgtaaatggt cggttagggt agagggtaag gttgattaag gtgctgctgt tacagagagt
     3481 gctgcaggat ctcctcgatg atccggcaag cggacttggt gtcctcgtca tggatgtcga
     3541 gatgagtgac caggcgcacg cgggtgggcg cggtggcgag acagagcacg cccttttctt
     3601 tcagtttaac aaccaaatca tgcgctttta atccagtttt tctcagttcc acaataacta
     3661 tattggtttg cgtagctgcg aggtcgacct caatttcggg gaattcgaga aaactgtggg
     3721 cgagggcgcg ggcgtggaga tgatcgacct tcagtcgatc gatatggtgg tcaagggcgt
     3781 aaaggccggc ggcggcgatg atgccggcct ggcgcatgcc gccgccccac attttgcgga
     3841 agcggtgggc cctgtcgata aaatcggccg atccggccag caccgagccg accggggcgc
     3901 cgagcccctt ggagaggcag accgagatcg aatcgaagag gcg
//
LOCUS       DAOPWU010000324         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_23460_length_3913_cov_4.231467, whole genome shotgun sequence.
ACCESSION   DAOPWU010000324 DAOPWU010000000
VERSION     DAOPWU010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23460_length_3913_cov_4.231467"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKM23_13000"
     CDS             complement(<1..714)
                     /locus_tag="PKM23_13000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY92435.1"
                     /translation="MLHNTATDKFSKLYWDLENRRPLTPGEGCPLDQWLTGRTEARFA
                     IHPAGFLVFLPPDQLEGVDEYRAGDPYAAEEGFVRWPAFQHRRLATTLKLLEKALASV
                     SGTPAILDVACGEGIITAEIKKQFGSAEVCGIDYSISAITTAASLHSGVEFAVGDARR
                     LPYCPGRFDAVVLNNIWEHLPDPLNLLEGVKRILKPGGYAIISTPSRYRFRNMVRVVL
                     GLPVEFMSKEHVTEYTVGQV"
     gene            complement(739..1806)
                     /locus_tag="PKM23_13005"
     CDS             complement(739..1806)
                     /locus_tag="PKM23_13005"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNY92436.1"
                     /translation="MNRVPRDVLPDYLHRLLCTSRRILYWALPPLIFWIILRSVDLPR
                     LGQLASGGHPALIILGLSLLVPVVMLGALRWHLLLHHYGCGRCTYRKSIREYWQGLAV
                     GLLAPGTLGFDAYRILLTGERSGYYLRCAFIILIEKAGALFSCALLVAGLFPLLGPNR
                     LPEGIARVVDALYLLLLAGGAGTAALLLLRRRRWAGAIAAAVNARLEAMTRRLNALAH
                     GQPARGEEAREEGSQRTGAALIWSAFDAEVALPAAGLSLAIHLVSALQSQILFLAYGH
                     QISFIVNLFIVPLLFLLYALPISFGGLGIREGAFILAYSAFGVPAETALVISLTGLAG
                     NLISYGIGALLFYPGGGTRRL"
     gene            complement(1803..3089)
                     /locus_tag="PKM23_13010"
     CDS             complement(1803..3089)
                     /locus_tag="PKM23_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNY92437.1"
                     /translation="MSLITLTTSLLGLLADSGRSSSDLAGTMQNRVRKLMMAAATVPR
                     HREAMQRTGYDPQHDFSGIADLSRLPVMTKEELKAAPENFLQEDYAHRLAGCFSDRTS
                     GSTGMPLTVCRSKREREIQVAKWMRLLLLSGYCPTDKVFSFTSPGRLDEGRSMLQQLG
                     LLRRHAVDYTLPPEVLTDELLRCRPAVVYGVRTSLLMVADELAKRSSQAPAVKLLLAG
                     GEVIDASTRRRCREAFGVEITETYGTVEMGVMAWQQKGREGLSLISDCTLFEFLDRKG
                     DPAAPGQWARIIVTDLHGWLMPFIRYDQGDLATYKIVEDERGESVQVIDRIIGRQDDI
                     ATLPDGRSLTYLDFYETMDNYAGIRQFRIRQQAPDRFLVELAAEAAYYRAIENELLNK
                     LARLSPLPLAFEIRLAERIPPDSSGKMRMLVQDFKT"
     gene            complement(3235..>3913)
                     /locus_tag="PKM23_13015"
     CDS             complement(3235..>3913)
                     /locus_tag="PKM23_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92438.1"
                     /translation="HYRKNNLPAPKLHLLIPGAEKIGKADRAFLADTFRPVRYCEFYG
                     ANETNLIAARFDEFYQPDYRAVFFSLADIVPQSEDGLVSGSMLVTSLVNEAQPILKME
                     LGDRVLVRNYDRLTRLGAAIVEIEGRNNDYLILPDGEKISGATLYIALEKLPFMRQFR
                     VVQDSAAACTVLLKIADLDAAKRLQIEELLQNLFMERIRYRIEYVEEIPIDPNGKTKI
                     IISRMKP"
BASE COUNT          801 a         1145 c         1209 g          758 t
ORIGIN      
        1 tacctgtccc accgtgtact cggtcacatg ctccttcgac atgaattcta ccggcaggcc
       61 caggaccacc ctgaccatat tgcggaaacg gtagcggctg ggcgtggaga tgatcgcata
      121 tccccccggt ttcaggatcc gcttcacccc ctccagcaga ttaaggggat cggggagatg
      181 ttcccagata ttgttgagga ccaccgcgtc gaagcgccct gggcaatagg gcagccggcg
      241 tgcatcacct acggcaaact cgaccccgct gtggagactg gcggctgtgg tgatcgctga
      301 gatggaatag tcgatgccgc atacctctgc gctgccaaac tgcttcttga tctcggctgt
      361 gataatcccc tcaccgcagg ccacatccag aatcgccggg gtaccagaga ctgaagccag
      421 ggccttctcc agcagtttca gggtggtagc aagccttcga tgctggaatg ccggccatct
      481 gacgaatccc tcttcggctg catagggatc tcctgcgcga tactcgtcaa cgccctccag
      541 ttgatcaggc ggcagaaaga ccagaaagcc ggcaggatgg atggcgaatc tcgcctccgt
      601 ccgcccggtc agccattggt cgagtggaca gccctcgccg ggcgtcaggg ggcgtcgatt
      661 ttctagatcc cagtaaagtt ttgaaaattt gtctgttgct gtgttgtgca gcatctttcc
      721 gctccaatgc gacaaagctc acaaccggcg ggttccgccg cccggataga agaggagggc
      781 gccgatcccg tagctgatca ggttgcccgc cagtccggtg agactgatga ccagggcggt
      841 ttccgccggc actccgaagg cgctgtaggc aaggatgaag gccccctcgc ggatgcccag
      901 tccgccgaaa gagatcggca gtgcgtaaag caggaagagc aaggggacga tgaaaaggtt
      961 gacgatgaaa gatatctggt ggccgtaggc cagaaaaagg atctgggact gcagggcgga
     1021 caccagatgg atggccagcg agagtcccgc cgccggaagg gcgacctccg catcaaacgc
     1081 ggaccagatc agggccgccc cagtgcgctg gctgccctcc tcccgggcct cttccccgcg
     1141 agccggctgg ccgtgtgcga gggcattgag tcggcgggtc atcgcttcga ggcgggcgtt
     1201 gaccgctgct gcaatcgcgc cggcccatct tcgccgccgg agaaggagca gggccgcagt
     1261 accggctccc ccagcgagca ggaggaggta gagggcatcg accacacggg cgataccctc
     1321 gggcagcctg ttcggaccca gcagcggaaa gagtcccgcg accagaagcg cacaggaaaa
     1381 gagcgctccc gctttctcaa tcaggataat gaaagcgcag cggagatagt atccgctgcg
     1441 ctctcctgtg agcagaatgc gataagcgtc gaatccgagg gtgccagggg cgagcagacc
     1501 cacggccagt ccctgccagt actccctgat gctcttccga taggtgcagc ggccgcagcc
     1561 gtaatggtgc agcagcagat gccagcgcag cgcacccaac atgaccacag gaacaaggag
     1621 gctgagcccg agaatgatca gggccggatg gccgccggaa gccagctgcc ccagccgggg
     1681 cagatcgaca ctcctgagga ttatccagaa tatcagcgga ggaagagccc agtagaggat
     1741 tcgccggctg gtacacagca ggcggtggag gtagtctgga agcacatcgc gcggcacccg
     1801 gttcatgtct taaaatcctg cacgagcatg cgcatcttgc cgctggaatc cggagggatc
     1861 cgctccgcaa gccggatttc gaaagcgagt gggagggggg agagtcttgc cagcttgttc
     1921 aataactcgt tctcgatggc gcgataataa gccgcttcgg ccgccagctc gacaagaaag
     1981 cggtcggggg cctgctgccg gatacggaac tgccggatcc ctgcatagtt gtccatggtc
     2041 tcgtaaaaat ccagataggt caggctgcgt ccatccggaa gggtggcgat gtcatcctgc
     2101 cggccgatga tccggtcgat cacctgcacg ctttctcctc tctcgtcctc cacgatttta
     2161 taggtggcca ggtcgccctg atcataacgg atgaagggca tcagccagcc gtgcaggtcg
     2221 gtgacgatga tgcgcgccca ctggcccggg gctgccggat cgcccttgcg gtcgagaaac
     2281 tcgaagaggg tgcagtcact gatcaggctg agcccctccc ggcccttttg ctgccaggcc
     2341 atcaccccca tctcaacggt tccataggtc tctgtgattt ccaccccgaa ggcttcgcgg
     2401 cagcgacgcc tggtcgatgc gtcgatcacc tcaccgccgg caaggagcag cttgactgca
     2461 ggggcctgcg agctgcgttt ggccagttca tctgcgacca tgagcagact ggtgcgcacg
     2521 ccatagacca ccgccggtcg gcagcgcagc agctcatcgg tgagaacctc cgggggcagg
     2581 gtgtagtcga ctgcatggcg tcgaagcaga ccaagctgct ggagcatgct gcgcccttca
     2641 tcaagccggc ccggcgaggt gaaagagaag accttgtcgg tcggacagta gccgctcagc
     2701 aggaggaggc gcatccattt ggccacctgg atctcgcgct ctcgttttga ccggcagacg
     2761 gtcagaggca tccctgtcga gcctgatgtc cggtccgaaa aacagccggc cagccggtgt
     2821 gcgtagtcct cctgcagaaa attttccggt gcggccttga gctcctcttt ggtcatcacc
     2881 ggcaggcggg aaagatcagc gattccggaa aagtcgtgct ggggatcgta gccggtgcgc
     2941 tgcatggcct cgcggtggcg cggcacggtg gcggccgcca tcatcagctt gcggactcga
     3001 ttctgcatgg ttccggcgag gtcggaagag gagcggccgg agtcagcgag caaccccagc
     3061 agggatgtcg tgagagtgat caggctcaag gcgtggtgtt tcctggcatc ccgcaataat
     3121 ttaatctctg cgggttggtc aatatttgtg tacgcgtgtt catcagaccc ggcttgattc
     3181 ccgcgacccc ttcggcaatt cttttgctga tggtagaata attccgggtg cgagtcatgg
     3241 tttcatccgt gagatgatga ttttggtttt gccgttggga tcgatgggaa tctcctccac
     3301 atactcgatc cggtatcgta tcctctccat gaaaaggttc tgcagcaact cttcaatctg
     3361 caagcgtttc gccgcatcaa gatctgcgat tttaagcaac actgtgcatg cagcggccga
     3421 atcctgtacg accctgaact gccgcataaa gggcaatttt tcgagagcga tgtacaaggt
     3481 cgcgcctgaa attttctctc cgtccgggag gatgaggtaa tcattattcc ggccttcgat
     3541 ttcaacgata gccgcaccca gccgggtcag ccggtcatag ttgcgcacca gcacccggtc
     3601 gccgagctcc attttgagga tgggttgggc ttcattgacc agcgaggtga ccagcatgga
     3661 gccgcttacc agaccgtcct cgctctgcgg gacgatatcg gcaagcgaga aaaagaccgc
     3721 ccggtaatcc ggctggtaga actcatcgaa gcgtgcggcg atcaggtttg tttcatttgc
     3781 gccgtaaaat tcgcagtagc ggacgggacg aaaggtatcg gccagaaaag cccggtcggc
     3841 ttttccaatc ttttccgccc ccggaatgag caggtgcagt ttcggggcgg gcagattgtt
     3901 cttcctgtaa tgc
//
LOCUS       DAOPWU010000325         3903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_23534_length_3903_cov_4.813410, whole genome shotgun sequence.
ACCESSION   DAOPWU010000325 DAOPWU010000000
VERSION     DAOPWU010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23534_length_3903_cov_4.813410"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKM23_13020"
     CDS             complement(<1..540)
                     /locus_tag="PKM23_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92439.1"
                     /translation="MRFSSKLMVSLAFVLSFLLLLGAQVTYAQNEKMSMDEYRAQLKD
                     WQDREVAAKKAADECANAVAGMKTEMTAAQAEAGQMWGEILTTVGTDQAGYDAYKAKL
                     TALEAKLDGLLALTPEELFKKRDELTAAEAELAELKKDKLSLLTEMQNALAGLEGKIA
                     QLKNKMPKALYDMYTVLPGD"
     gene            complement(585..863)
                     /locus_tag="PKM23_13025"
     CDS             complement(585..863)
                     /locus_tag="PKM23_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92440.1"
                     /translation="MKSTSRWIPRIAAVCLLLFAFMLVAGCHNHPNEKELKALEEAKQ
                     AALSAETKAADCAKEKASLEQQLTQQKQKVESLKQEKAAVEKRLSAWQ"
     gene            complement(1014..2804)
                     /gene="aspS"
                     /locus_tag="PKM23_13030"
     CDS             complement(1014..2804)
                     /gene="aspS"
                     /locus_tag="PKM23_13030"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNY92441.1"
                     /translation="MKNKRTHTCGDLRPAHLGHTVTLMGWVDRRRDHGSLIFLDLRDR
                     YGITQVRIDPDSPGYETAKQLRGEYVIAVTGVVEPRPEGMVNARLATGAVEVAAVEIE
                     LLNVARTPPFMISGESIANEDLRLKYRYLDLRRPEMQRSMLIRHQSYQIVRSYFAGKG
                     FVEIETPILMRSTPEGARDYLVPSRVWKGRFYALPQSPQQYKQLLMVAGYDRYFQIVR
                     CFRDEDLRADRQPEFTQIDVEMSFVDEEDIFAMVEELMAELFRSTLGIELPMPIQRIP
                     YDEALARYGSDKPDLRFGLEIREISALVADAEFKVFRETIAAGGVVAGLCAPGCGGYS
                     RKQIDELTEWVKSMGGAGLVAIKLKEGDWESSLSKFFSEGQRHSIMQAMGATGDDLLL
                     LVADEREKALLLLGDLRLKIAAQEKLIPENLFKLAWVVDFPLFEYSPDDQRYVARHHP
                     FTSPKPGDEELMLSDPARARARAYDLVLNGMEVAGGSIRIHRSDLQSRMFEALGIGAV
                     EAQEKFGFLLEAFAYGAPPHGGIAFGFDRLVMILAGRQSIREVIAFPKTASAMSLMDG
                     SPSAVNPAQLQELGLLLERQASEAGPEPGK"
     gene            complement(2953..3357)
                     /locus_tag="PKM23_13035"
     CDS             complement(2953..3357)
                     /locus_tag="PKM23_13035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNY92442.1"
                     /translation="MDKTFILEIVTPVKVVCKEEVLHVKAPGVGGYFGVLYNHAPFLT
                     ALRIGVIEAKTPTGMHYYATSGGFCEVMENTMKILVETAEPAVEIDIERAKQARDRAL
                     ARLEKKSPDIDVPRAQGALARAINRLEVAEMR"
     gene            complement(3406..>3903)
                     /locus_tag="PKM23_13040"
     CDS             complement(3406..>3903)
                     /locus_tag="PKM23_13040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948371.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNY92443.1"
                     /translation="VPADDLTDPAPATTFSHLDATTVLSRQLAEMSIYPAVDPLDSTS
                     RILDPRIVGEEHYSVARSVQQILQKYKDLQDIIAILGMEELSEEDRITVNRARRLQRF
                     LSQPFAVAEAFTGFPGRYVKLEDTIRGFKEIVEGKHDDLPERAFWMVGTIEEAVENAK
                     KIREA"
BASE COUNT          775 a         1174 c         1117 g          837 t
ORIGIN      
        1 gtcaccggga agaacggtgt acatatcata gagcgccttc ggcatcttgt tcttcagctg
       61 ggcgatcttg ccttccagac ctgccaaggc attctgcatc tcggtgagca gggagagttt
      121 gtcttttttc agctcggcga gctcggcttc ggcagccgtg agctcatcgc gctttttgaa
      181 caactcttcc ggggtgagag ccagcaggcc gtcgagtttg gcttcaaggg ccgtcagttt
      241 ggctttatag gcatcgtacc cggcctgatc ggtgccgacc gtcgtgagaa tctcgcccca
      301 catctggccg gcttcggcct gagcagcggt catctcggtt ttcatacctg caacagcatt
      361 tgcacattca tccgccgcct ttttggcagc gacttcgcga tcttgccagt ctttcagttg
      421 agcacggtat tcatccatgc tcattttctc attctgcgca taggtcacct gcgcgcccaa
      481 aaggagcaag aaggaaagca caaatgctaa agagaccatg agcttgctag aaaatctcat
      541 tagcttccct ccactgtttt gtgaatgtat catggactgt agaattattg ccaagcggaa
      601 aggcgtttct caaccgcggc tttctcttgc ttcaagctct caactttctg cttctgctgc
      661 gtcagctgct gctccagact ggccttctct ttggcgcagt ccgcagcctt ggtttccgct
      721 gacaaagccg cctgcttggc ctcttccagc gccttcagct ccttctcatt ggggtggtta
      781 tgacatccag ccaccagcat aaaagcgaaa agcagcagac aaacagcggc gattctggga
      841 atccagcgtg atgtgctctt catgcaatac acctcctttc aatccattgc ttcaagccct
      901 tgtaggtgct tgtcgaataa agatatgtga aaaatacaca tctcacccca ccgtaagaac
      961 ttttctgtgc tcattttaac aataatttgg ctaattgtca aggtaaaaga tatttacttg
     1021 cccggttcag gaccggcttc ggaggcttgc cgctcgagca gcagccccag ctcctgcagc
     1081 tgcgccggat tgaccgccga gggcgagcca tccatcaacg acatggcgct ggctgttttt
     1141 ggaaaggcga tgacctcgcg gatcgactgc cggccggcca ggatcatcac caggcggtca
     1201 aaaccaaagg cgatgccgcc atggggcggg gcgccgtaag cgaaggcctc gagcaggaag
     1261 ccgaatttct cctgcgcctc gacagcgccg atgcccaggg cctcgaacat tctcgactgg
     1321 agatcactgc ggtggatgcg gatgctgccc cccgccacct ccatgccgtt gaggaccaga
     1381 tcgtaggcgc gggcccgggc ccgggccgga tcgctgagca tcaactcctc atccccgggt
     1441 ttgggcgagg tgaaaggatg atggcgcgcg acataacgct ggtcatccgg actgtactcg
     1501 aagagcggaa aatcgaccac ccaggccagt ttgaagagat tttcgggaat gagcttttcc
     1561 tgggcggcga tcttgaggcg cagatcaccg aggagcagca gggctttctc gcgctcgtcc
     1621 gcgaccagaa gaagaaggtc gtcgccggtc gcgcccatcg cctgcatgat gctgtggcgc
     1681 tggccttcgc tgaaaaattt gctcagtgag ctctcccagt cgccctcctt gagcttgatc
     1741 gccaccaggc cggccccgcc catggatttt acccattcgg tcagctcatc gatctgcttg
     1801 cgcgagtagc cgccgcagcc cggcgcacag aggccggcca ccacaccacc agcggcgatc
     1861 gtctcacgaa agaccttgaa ctcggcatca gcgaccaggg cggagatctc cctgatctcc
     1921 agcccgaaac gcaggtccgg cttgtcactg ccgtagcggg cgagagcctc atcatagggg
     1981 atgcgctgaa tcggcatggg cagttcgatg cccagcgtgc tgcggaaaag ctcagccatc
     2041 agctcttcga ccatggcgaa aatgtcttcc tcgtcgacga aggacatctc gacatcgatc
     2101 tgggtgaatt caggctgccg gtcggcgcgc agatcctcat ccctgaaaca gcggacgatc
     2161 tggaaatagc ggtcataacc ggccaccatg agcagctgtt tgtactgctg cggcgattgc
     2221 gggagggcgt aaaatcgacc cttccagacc cggctcggca ccagataatc gcgcgcccct
     2281 tccggcgtgc ttctcatcag gatcggggtc tcgatctcga caaagccctt gccggcaaaa
     2341 tagctgcgca cgatctgata gctctggtgg cggatgagca tgctgcgctg catctcgggc
     2401 cggcgcagat cgagataacg atacttgagg cgaagatcct cgttggcgat gctctcgccg
     2461 ctgatcatga agggcggggt ccgggcgaca ttgagcagct cgatctccac tgcggctacc
     2521 tcgactgccc cggtcgccag ccgggcattg accatcccct ccgggcgggg ttcaaccaca
     2581 ccggtgaccg cgatgacgta ttcaccgcgc agctgcttgg cggtctcata accgggacta
     2641 tccggatcga tgcggacctg ggtgatgccg taccggtcgc gcagatcaag aaagatcagt
     2701 gaaccatgat cgcggcgccg gtccacccat cccatcaggg ttaccgtatg gcccagatgg
     2761 gccggccgca gatcgccaca ggtatgtgtt cgtttatttt tcatatcagg atagatctcc
     2821 ataatcggtt gcactctgaa tgtgatcatg acccaaaaaa gaaaacggca cccatgccgg
     2881 gcgccgttct gaacgggaat catttcctgc agagcgggca cagcatgcgg ttgcgcccgc
     2941 ttcccgagaa gctcagcgca tctccgcaac ctccagccgg ttgatagcgc gcgccagggc
     3001 accctgggcg cgcggcacgt cgatgtccgg ggattttttc tccagccggg ccagggcccg
     3061 atcgcgcgcc tgcttggcgc gttcgatgtc gatctctaca gccggctcag cggtctcaac
     3121 gagtatcttc atcgtgtttt ccatcacctc gcaaaaaccg ccgctggtgg cgtaataatg
     3181 catccctgtg ggggttttcg cttcgatgac gccgatcctc agggcggtca gaaagggggc
     3241 gtgattgtag agcacgccaa agtacccgcc gacaccgggg gctttgacgt gcagcacctc
     3301 ttccttgcag accaccttga ccggggtcac gatctccagt atgaaagtct tgtccataag
     3361 ttcttgtctt gaacccgata attgggatga tgcccggcag cgggcttagg cctcgcgaat
     3421 cttcttggcg ttctcgacgg cctcttcaat ggtccccacc atccagaagg ctctctccgg
     3481 cagatcatcg tgcttgccct cgacgatctc cttgaagccc cggatggtat cttccagctt
     3541 gacataacgg cccggaaaac cggtgaaggc ctcggcgact gcaaagggct gcgagaggaa
     3601 gcgctgcagc cggcgggcgc ggttgacggt gatgcggtcc tcttccgaga gctcttccat
     3661 gccgaggatg gcgatgatat cctgcagatc cttgtatttc tgcagaatct gctgcacgga
     3721 gcgggcgacg ctgtagtgct cctccccgac gatgcgcggg tcaaggatgc gcgaggtcga
     3781 atccagggga tccacagccg ggtagatgct catctccgcc agctggcgcg agagtacggt
     3841 ggtggcatcc agatggctga aggtggtcgc cggagccgga tcagtgaggt catcggccgg
     3901 cac
//
LOCUS       DAOPWU010000326         3882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_23715_length_3882_cov_4.754115, whole genome shotgun sequence.
ACCESSION   DAOPWU010000326 DAOPWU010000000
VERSION     DAOPWU010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23715_length_3882_cov_4.754115"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKM23_13045"
     CDS             <1..412
                     /locus_tag="PKM23_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNY92444.1"
                     /translation="RQKQQTQRVFNIVMGAIAGISLLVGGIGIMNIMLASVMERTREI
                     GVRRAVGARQRDILGQFLVEAMVLSFAGGLLGVLLGFILTKVIALYAGWRTLVSLSAI
                     ILAFTVSAAVGIVFGIYPARQAARLDPIVSLRYE"
     gene            445..2073
                     /locus_tag="PKM23_13050"
     CDS             445..2073
                     /locus_tag="PKM23_13050"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNY92445.1"
                     /translation="MVQIRFFPVHVLISIALLTAGGRGWCQESSAPALGDSSSVAAPE
                     HPALQAAGAEKATLTLDEAIKIALEQSYEMKSLRLSLNQAAQNLIAAKGRFKTNADLS
                     LEAPNWNETVSEIQVPGALPVFNTTGYTRFLGTLDINQPLPTDGAFTLRGQSYHRDVS
                     TYLQEQDEDVSRSEIYNSISLRFKQPLFAINQLKLGLKNANLSYERTFQRFRRSELDI
                     VYSVTRSFFDYYQATRRHEITSENLNQQQELYDLAAKKYAAGLIPEVEALQMEVDLAE
                     SRAALLSAEGARQRSEDNFKQLIGLQLSDAVAVRTDFELEKVKVDLERATALAMKYRT
                     ELREGQIDIEMARLDVKQADARSEMRGDIAAFYDVTGVSNSSLPYKSPWDRLWDSSIE
                     DMDRRPHNRGVTFTLTVPLWDWGVNHAEVQSARAALSTSELSLEEQKKTIQREVRDVV
                     MRLREAENRVEVLKKNQEVAQRAFDITLERFNNGDITSQDLALDRNRLTTAKTSYLEA
                     YVDFKLAVADLQRKTMWDFVLDRSLLDTAPEAGK"
     gene            2176..3486
                     /locus_tag="PKM23_13055"
     CDS             2176..3486
                     /locus_tag="PKM23_13055"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNY92446.1"
                     /translation="MKLTVIGSGYVGLVAGACFAEAGNQVICMDVDEKKIAGLRKGLI
                     PIYEPGLEGLVERNHKLERLSFTTSIKDAVEQSDILFIAVGTPPEEDGSADLSHVLAV
                     ARSIGKLMNGYKIVVNKSTVPVGTADRVREAIAAETDLPFDVVSNPEFLKEGAAIDDF
                     MYPDRVIIGTRNPKVGEIMRELYQPFVRTGNPILVMDEHSAEVTKYAANSLLATKISF
                     INEIANLCDRVGADVEMVRRGIGSDKRIGHSFIFPGMGYGGSCFPKDVKAIIHTGKQY
                     GCRLKVLEAVEAANEEQKTILLPKIREHFQGKLAGLTLALWGLSFKPRTDDMREAPAI
                     PLINALDAEGVKLQAHDPAALHEARRLGLDKKVRLFENNYEALQGADGLVIVTEWLDY
                     REPDFERIRKALNTPVIFDGRNIYNPVKIRDLGFSYYAIGRPQA"
     gene            complement(3554..>3882)
                     /locus_tag="PKM23_13060"
     CDS             complement(3554..>3882)
                     /locus_tag="PKM23_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HNY92447.1"
                     /translation="PTAVLKSAAKMDHVRTGGTLLNQKFTPSLLKGEEGIDHLSHLVR
                     GYFTLGGHHIQFNVVDAATLRHAQAHPEEHRDLIVRVAGYSDYFLDLSKALQDEIIAR
                     TEHQGF"
BASE COUNT          786 a         1187 c         1155 g          754 t
ORIGIN      
        1 gcgtcaaaag cagcagaccc agcgcgtctt caacatcgtc atgggggcca ttgccggtat
       61 ctccctgctg gtggggggga tcggcatcat gaacatcatg ctcgcttcgg tgatggaacg
      121 gacccgggag atcggggtgc ggcgcgcggt cggggcccgg cagcgcgaca tcctcggcca
      181 gtttctggtc gaggccatgg tgctcagttt cgccgggggg ctgcttggag tgctgcttgg
      241 cttcattctg accaaggtta tcgccctcta tgcaggctgg cgcaccctgg tcagcttaag
      301 cgccatcatt ctggctttca ccgtctcggc cgccgtcggc atcgtattcg gcatttatcc
      361 agcccggcag gcggccaggc tcgatccgat cgtttccctg cgttatgaat agcggcttgc
      421 cgcgtgctaa ttcttgagga gcatatggtt cagattcgct ttttccccgt acatgtcctg
      481 atctccatcg cgcttctgac cgccggcggg cggggttggt gccaggagag ttctgcgccg
      541 gcgcttggcg actcttcgtc cgtcgctgcg ccggagcatc ccgctctcca ggctgcagga
      601 gctgaaaaag ccaccctcac cctcgacgag gcgattaaaa tcgccctcga gcagagttat
      661 gagatgaaat ccctgcgcct gagtctgaat caggcggcgc agaatctgat cgccgccaag
      721 gggcgcttca agaccaacgc cgatctcagc ctggaggcgc cgaactggaa cgagaccgtc
      781 tccgaaatcc aggtgccggg tgccctgccg gtctttaata ccaccggcta cacccgcttc
      841 ctcggcaccc tcgatatcaa ccagcccctg ccgaccgacg gcgccttcac cctgcgcggc
      901 caaagctacc accgcgatgt ctcgacctat ctccaggagc aggatgagga cgtctcgcgc
      961 agcgaaatct acaactcgat cagcctccgc ttcaaacagc ccctcttcgc gatcaatcag
     1021 ctcaaacttg gattgaagaa cgccaacctc agctacgagc gcacctttca gcgctttcgg
     1081 cgcagcgagc tggatatcgt ctacagcgtc acccgctcct tctttgatta ttatcaggcg
     1141 acccgacggc atgagatcac cagtgaaaac ctcaaccagc agcaggaact ctacgacctg
     1201 gcggcaaaaa aatatgcggc cggcctcatc cccgaggtcg aggccctgca gatggaggtc
     1261 gatctggcgg aaagccgggc cgccctgctc tcggctgaag gagcccggca gcgcagcgag
     1321 gacaatttca agcagctgat cggactccag ctctccgatg ctgtggccgt gcgcactgat
     1381 ttcgagctcg aaaaggtcaa ggttgatctg gagcgagcta cggcactggc gatgaaatac
     1441 cgcaccgaac tgcgcgaggg gcagatcgat atcgaaatgg cccggctcga cgtcaagcag
     1501 gccgatgcgc gcagcgaaat gcgcggcgac atcgccgcct tttacgacgt caccggggtg
     1561 agcaattcca gtctgcccta taaatcaccc tgggaccggc tgtgggattc gagtatcgag
     1621 gatatggacc gccgtccgca caaccgcggc gtcaccttca ccctgaccgt gccactctgg
     1681 gattgggggg tcaaccacgc cgaggtgcag tcggcgaggg cggccctttc gaccagcgaa
     1741 ctgagcctgg aggagcagaa gaaaaccatc cagcgcgaag tgcgcgacgt ggtcatgcga
     1801 ttgcgtgagg ccgaaaaccg cgtcgaggtg ctgaaaaaga accaggaggt ggcccagcgc
     1861 gccttcgaca tcaccctcga gcgcttcaac aatggcgata tcaccagcca ggacctggcc
     1921 ctcgaccgca accgactgac cacggccaag acctcctatc tcgaggccta tgtcgatttc
     1981 aaactcgcgg tagcagacct ccagcgcaag accatgtggg attttgtcct tgaccgcagc
     2041 ctgctggata cggcgccgga agcggggaaa taagttctga ccgcatttcc ctctctgcaa
     2101 accggccctg ctgcgtgtag aataggatga ttaaagaagc gttttcttcg cggacattaa
     2161 cttgatggga cgggtatgaa actgacagtc atcggttccg ggtatgtggg tttggtggcg
     2221 ggggcgtgct ttgctgaagc cggcaatcag gtcatctgca tggatgtgga cgaaaaaaag
     2281 atcgccggcc tccgcaaggg attgatcccg atctatgaac ccggtcttga gggactggtc
     2341 gaacgcaatc acaaactgga acggctctct ttcaccacct cgataaagga tgcggtcgag
     2401 cagtcggata tcctcttcat cgcggtgggc actccacccg aggaggacgg ctcggcggat
     2461 ctcagccacg ttctggcggt cgcccgctcc atcggcaagc tcatgaacgg ctacaagatc
     2521 gtcgtcaaca agagcacggt tccggtgggc acggcggaca gggtgcgcga ggccatcgcc
     2581 gcggagaccg atctcccctt cgacgtcgtc tccaatccgg agtttctcaa ggagggggct
     2641 gccatcgatg atttcatgta tcctgaccgg gtcatcatcg gtacccgcaa tcccaaggtg
     2701 ggggagatca tgcgcgaact ctaccagccc tttgtccgca ccggcaatcc gatcctggtc
     2761 atggatgagc acagcgcgga ggtgaccaaa tacgccgcca attccctgct ggcgaccaag
     2821 atctccttca tcaacgagat cgccaacctc tgtgaccgcg tcggcgccga tgtggagatg
     2881 gtgcgccgcg gcattggatc ggacaagcgc atcggccact ctttcatttt tcccggcatg
     2941 ggttacggcg gctcctgctt tcccaaggat gtcaaggcga tcatccatac cggcaagcag
     3001 tatggctgcc gcctcaaggt cctggaagcg gtcgaggccg ccaatgagga acagaagacg
     3061 attctgctgc ccaagatccg ggagcatttt cagggcaagc tggccggcct caccctcgcc
     3121 ctctgggggc tcagcttcaa gccgcgcacc gacgatatgc gcgaagcccc ggccatcccc
     3181 ctgatcaacg cccttgacgc cgaaggggtc aagcttcagg ctcacgatcc cgccgccctc
     3241 catgaggccc ggcggttggg cctcgacaag aaagtcaggc tgttcgaaaa caactatgaa
     3301 gccctgcagg gcgctgacgg tctggtgatt gtcactgaat ggctcgatta ccgggaacct
     3361 gatttcgagc gcattcgcaa ggccctcaat actccggtca tcttcgacgg ccgcaatatc
     3421 tacaatccgg tcaagatccg cgatctcggt ttcagctatt atgccatcgg ccgcccgcaa
     3481 gcctgaccca gccagcacgc agacaaaaaa gcctcccggg gaattccccg ggaggctttt
     3541 ttattggagt tggctaaaag ccctgatgtt ccgtgcgggc gatgatttca tcctgcaggg
     3601 ccttgctgag gtcgaggaaa tagtcgctgt agccggcgac gcggacgatg agatcgcggt
     3661 gctcttcggg gtgagcctgg gcgtggcgga gggtggcggc gtccaccaca ttgaactgga
     3721 tatgatggcc gccgagggtg aaatagccgc gcacaaggtg gctgaggtgg tcgattcctt
     3781 cctccccctt gagcagggag ggggtgaact tttggttgag cagggtgccg ccggtgcgga
     3841 cgtgatccat cttggcggcc gatttcagca ccgccgtcgg cc
//
LOCUS       DAOPWU010000327         3871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_23791_length_3871_cov_4.301101, whole genome shotgun sequence.
ACCESSION   DAOPWU010000327 DAOPWU010000000
VERSION     DAOPWU010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23791_length_3871_cov_4.301101"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKM23_13065"
     CDS             complement(<1..392)
                     /locus_tag="PKM23_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92448.1"
                     /translation="MAVGLKRERHSFSKIKSIADIPDLLDIQLKSFRDFLQADVPPDQ
                     RRDVGLQSVFKSVFPIIDSRENFVLDFIEYYIDPPKYSVEECQERGMSYSVALKAKLR
                     LAIKDEYNPNDPLANSKEKFERISQKAAA"
     gene            complement(472..891)
                     /gene="rplL"
                     /locus_tag="PKM23_13070"
     CDS             complement(472..891)
                     /gene="rplL"
                     /locus_tag="PKM23_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNY92449.1"
                     /translation="MPEKADVAVAQDAKVEEILTAIEQMSVLQLSKLVKAVEERFGVT
                     AAAPMAMPAGMMMGGAAAAAPVVEEKTEFDVVLKAAGANKINVIKVIRTITNLGLKEA
                     KDLVDGAPSKVKEAVTKDEANDIKAKLEEAGAEVELK"
     gene            complement(925..1443)
                     /gene="rplJ"
                     /locus_tag="PKM23_13075"
     CDS             complement(925..1443)
                     /gene="rplJ"
                     /locus_tag="PKM23_13075"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNY92450.1"
                     /translation="MPQANKVAKLEEINASLEKAKSVFLTDFSGLTVEEVTRLRKEFR
                     KANVKYLVVKNTLARISAKENGYEALVPFLQGPTGMAIAMDDPVAPVRIIFDFKKDKE
                     KPSIKAAFLEGELLDQKSAEEVRNIPTREVLLAQVASAFAAPLSGFVGGLKAVISNFA
                     NVLNQVQEKKEA"
     gene            complement(1482..2183)
                     /gene="rplA"
                     /locus_tag="PKM23_13080"
     CDS             complement(1482..2183)
                     /gene="rplA"
                     /locus_tag="PKM23_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNY92451.1"
                     /translation="MMKHSKRYSEALQQLDRRNSYPLTEAVVKVKELATARFDESIEL
                     SVRLGVDPRKADQMVRGTVILPHGTGKKVRVCVLTKGAKEKEAQEAGADVVGSDELIT
                     RIQGGWLEFDVVIATPDIMSQVGKLGKILGTRGLMPNPKSGTVTFEVGQAVRDAKAGK
                     IAFRVDKYGIVNAAVGKKSFEPGQLKENVQAFMEMINRLKPTSAKGVYIKSVTLSSTM
                     SPGIRLDKSQIMDAV"
     gene            complement(2227..2649)
                     /gene="rplK"
                     /locus_tag="PKM23_13085"
     CDS             complement(2227..2649)
                     /gene="rplK"
                     /locus_tag="PKM23_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNY92452.1"
                     /translation="MAKKVVGSIKLQIPAGAANPSPPVGPALGQHSVNIMEFCKQFNA
                     RTQEKAGLIIPVIITVYADRSFSFITKTPPAAILLKKAAGLDKGSGEPNRTKVGQVTR
                     DQVREIAELKMQDLNASTVETAMSMVEGTARSMGITVV"
     gene            complement(2659..3198)
                     /gene="nusG"
                     /locus_tag="PKM23_13090"
     CDS             complement(2659..3198)
                     /gene="nusG"
                     /locus_tag="PKM23_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNY92453.1"
                     /translation="MEQEELRWYAIHVLSGHENKIKAYLENEIKHSNLYEKIDKILVP
                     SEEVTEMREGKKKVRTKIIFPGYMLVHMIIDKETRHLILNTPGVTNFVGSKNEPTPLQ
                     DDEADRILGRSEGAVKDRSRIPFHLGDPIKVVDGPFTDFTGFVEEVNEEKNKVKVMVS
                     IFGRATPVELDFLQIELEQ"
     gene            complement(3282..3464)
                     /gene="secE"
                     /locus_tag="PKM23_13095"
     CDS             complement(3282..3464)
                     /gene="secE"
                     /locus_tag="PKM23_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNY92454.1"
                     /translation="MFKKLANFVREVKTEMAKVSWPSKGQLKGQTLVVILVSLFFAVF
                     IFAIDHLLSRLLSLLY"
     gene            complement(3546..3619)
                     /locus_tag="PKM23_13100"
     tRNA            complement(3546..3619)
                     /locus_tag="PKM23_13100"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3583..3585),aa:Trp,seq:cca)
     gene            complement(3628..3777)
                     /gene="rpmG"
                     /locus_tag="PKM23_13105"
     CDS             complement(3628..3777)
                     /gene="rpmG"
                     /locus_tag="PKM23_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNY92455.1"
                     /translation="MRDLVILECSECKRRNYSTDKNKRKQPGRIEYKKYCRWCKKRTA
                     HKETR"
BASE COUNT          702 a         1081 c         1137 g          951 t
ORIGIN      
        1 gccgcggcct tttgcgagat ccgttcgaat ttctccttgg aattggccag ggggtcattg
       61 gggttgtact cgtccttgat agccaggcgg agtttggcct tgagagccac cgagtaggac
      121 atcccccgct cctgacactc ctcaacgctg tacttgggag ggtcgatgta gtactcgata
      181 aagtcgagga cgaaattttc ccggctgtcg atgatcggaa agacgctctt gaaaaccgac
      241 tgcaggccga catcccgccg ctgatcgggc ggtacatcgg cctggagaaa atccctgaat
      301 gacttgagct ggatgtccag cagatcgggg atatcggcga tggacttgat cttcgagaag
      361 gagtgtcgtt ctctttttaa gccaactgcc aaaggagaac tccttttgat gggtgacggg
      421 taagatcggg caagaaaaac cgggcgcaac gggcccggtt ttcagcttga actacttgag
      481 ttccacttcc gcgccggctt cttccagctt ggccttgatg tcgttggctt cgtccttggt
      541 cacggcttcc ttgaccttgc tcggagcgcc gtcgaccagg tccttcgcct ctttcaggcc
      601 gaggttggtg atggtgcgga tgaccttgat gacgttgatc ttgttggcgc cggcggcctt
      661 gaggacgaca tcaaactcgg tcttctcctc gacaaccgga gcggcggcgg cggcaccacc
      721 catcatcatg ccggcaggca tggccatcgg ggcggcggcg gtgacgccga aacgctcttc
      781 gacggctttg accagcttgg agagctggag gacactcatc tgctcgatgg ccgtgaggat
      841 ttcttcaacc ttggcatcct gagcaactgc gacatctgct ttttcgggca tttctacctc
      901 ccgttagagt actatatagg tggattaagc ttcttttttc tcttggacct gatttaacac
      961 gttggcgaaa ttggagatga ccgccttgag gccgccgacg aaaccggaga gcggggcggc
     1021 gaaggccgag gcgacctggg ccagcagcac ctcgcgggtc gggatgttgc gcacctcttc
     1081 ggccgatttc tggtcgagca gttcgccctc gaggaaggcg gccttgatcg agggtttttc
     1141 cttgtccttc ttgaaatcga agatgatgcg caccggcgcg accgggtcat ccatggcgat
     1201 ggccatgccg gtcggcccct gcaggaaggg gaccagggcc tcatagccgt tctccttggc
     1261 cgagatgcgc gcgagggtgt ttttcacgac cagatatttc acgttcgcct tgcggaactc
     1321 cttgcgcagg cgggtgacct cttcgacggt caagccggaa aaatcggtga gaaaaacgct
     1381 cttggccttt tcgaggctgg cgttgatctc ttccagtttg gcaactttgt tggcttgtgg
     1441 catggaggat atcctttact tcgagataac gtttatcggg ctcagaccgc gtccatgatc
     1501 tggcttttgt ccaggcggat gcccgggctc atggtgctcg aaagggtcac gctcttgatg
     1561 tagacgccct tggcagaggt gggtttgaga cggttgatca tctccatgaa ggcctggacg
     1621 ttctccttga gctggccggg ttcgaaggac tttttgccga ccgccgcgtt gacgatgccg
     1681 tacttgtcga cgcggaaggc gatcttgccg gccttggcat ccctgaccgc ctggccgacc
     1741 tcgaaggtga ccgtgccgct cttgggattg ggcatcaggc cgcgggtgcc gaggatcttg
     1801 ccgagcttgc cgacctgact catgatatcg ggggtggcga tgaccacgtc gaactcgagc
     1861 cagccgccct ggatgcgggt gatgagctcg tcggatccga cgacatcggc gccggcctcc
     1921 tgggcctctt tctccttcgc gcctttggtc agcacgcaaa cgcgcacctt tttgccagtg
     1981 ccgtggggca gaatcacggt gccgcgcacc atctgatccg ccttgcgcgg atcgacgcca
     2041 aggcggacac tcagctcgat cgattcatcg aaccgggccg tggcgagctc tttcaccttg
     2101 accaccgctt cggtcagggg gtaggagttc cggcggtcca gctgctgcaa cgcctcgcta
     2161 taacgcttgc tatgtttcat tattgctcca aaacacttcg aatcattaat tgttcgcgga
     2221 cttggctcaa acgacggtga tgcccatgct gcgagcagtc ccttcgacca tgctcatggc
     2281 ggtctcgacc gtgctggcgt tgagatcctg catcttgagt tcggcgattt cgcgcacctg
     2341 gtccctggtc acctggccca ccttggtgcg gttgggttca ccggagccct tgtccaggcc
     2401 tgccgccttt ttgagcagga tggccgccgg gggggtcttg gtgataaagg agaaggagcg
     2461 gtcggcgtag accgtgatga tcaccgggat gatcaggccc gctttttcct gggtgcgggc
     2521 attgaactgc ttgcaaaact ccatgatgtt gacgctgtgc tgacccagcg cagggcccac
     2581 cgggggtgat ggattggccg cgccggcagg aatctgcagt ttgatgctgc caactacttt
     2641 ttttgccatg agattacctt attgttctaa ttcgatttgg aggaaatcca gttcaaccgg
     2701 tgtggcgcgc ccgaagatgc tcaccatcac cttgaccttg tttttctctt cattgacctc
     2761 ttcgacaaag ccggtgaaat cggtgaaggg gccgtcgacc accttgatcg gatcgccaag
     2821 atggaagggg atgcgcgaac gatccttgac cgccccctcg ctgcggccga gaatgcggtc
     2881 cgcctcgtca tcctgcaggg gtgtgggctc gttcttgctg ccgacgaaat tggtcacccc
     2941 gggggtgttg aggatgagat ggcgggtctc cttgtcgatg atcatgtgca ccagcatgta
     3001 gccggggaag atgatcttgg tgcgcacctt cttcttcccc tcgcgcatct cggtgacctc
     3061 ttcggacgga acaagaatct tgtcgatctt ttcatagagg ttggaatgct tgatctcgtt
     3121 ctccagatag gccttgatct tgttctcatg gcccgagagg acgtgaatcg cataccatct
     3181 taattcttct tgttccactt gttccacttg ttcaatcctt tatgcccgta aaggggaagc
     3241 gcagtccacc acccgcccgc tccggcccgt gccggtatgg gctagtagag cagactcaac
     3301 agccgcgaga gcagatgatc gatcgcaaag ataaaaaccg caaagaagag cgagaccagg
     3361 atgaccacca gggtctgccc tttcagctga cccttgctgg gccagctgac cttggccatc
     3421 tcggttttga cttcgcggac aaaattggct agctttttga acatggcctc ggctctgttt
     3481 aggttgccag tgctacttca gggagcgccc ggccgggcgc tccctggttt ttttatcctc
     3541 tattgcaggt ctggagggac tcgaaccccc aaccgtcgga tttggagtcc ggtgctctac
     3601 catattgagc tacagaccta agccaagcta acgggtctct ttatgagccg tacgcttttt
     3661 acaccacctg caatattttt tatactcgat acgtcccggc tgcttgcgct tgttcttgtc
     3721 cgtggagtag tttcggcgct tgcattcgga acattccaga ataacgagat ctctcatgat
     3781 tgaacctgtt agctattaag gttgaaatcc agctacttga tgatcttggt caccacgccg
     3841 gcgccgacgg tgcggccgcc ctcgcggatg g
//
LOCUS       DAOPWU010000328         3855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_23906_length_3855_cov_4.887895, whole genome shotgun sequence.
ACCESSION   DAOPWU010000328 DAOPWU010000000
VERSION     DAOPWU010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23906_length_3855_cov_4.887895"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PKM23_13110"
     CDS             <1..325
                     /locus_tag="PKM23_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92456.1"
                     /translation="IRLQKGLSGLLLHALDQAGAAGSVRLSVEPDAEGGLAMRLVFSG
                     SAPGWSEPLPSQPPWRSAHLRLALAYAIIAELGGGFRMRRLHDGSTEIVILLAAEGKL
                     LSRPV"
     gene            386..1414
                     /locus_tag="PKM23_13115"
     CDS             386..1414
                     /locus_tag="PKM23_13115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNY92457.1"
                     /translation="MKERVQKFIVDRQLRLRHSFGDVLERTLGESLKSGQQLHRRLQA
                     LGLGDAAEALAEAVRHGRSAGGEKIFPSPLLALHWLAEPLVEGERIEGATIVLTDVTR
                     EKRQQHQQELGGRMEEVSTFAHKLASRLNNPLAAVLNGIGCLVLEEEPGPEWSRVRAE
                     LAELQEQVYAISRVTQALESFSREEGNSGKLVQLEAVLEKAVEVIQLLTAPRGVQISV
                     SAGPVSSLIYANDILLEQCLLHLLRNAVEASPEGGEVIIRCGSSDGMAAVSISDSGAG
                     IAAAEMKRIYEPFYSTKSTDHLGVGLPISYTIAARYKGFLELESVPGSGTTARLLFPV
                     AKNMVKKG"
     gene            1421..2914
                     /locus_tag="PKM23_13120"
     CDS             1421..2914
                     /locus_tag="PKM23_13120"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY92458.1"
                     /translation="MNDKKTSDRSILVIDDERTVLDSLVKMLKKSQYSCITATNAEDG
                     IRLFARERPLLVLSDLYMPNHKDGIQVLEEVRRIDPDAVVILYTAYSHIPDVVEATKK
                     GAFDYIQKVETHHDILLPIERGLKFARMQRENAYLRSRLDLTDDTLFYGAIGGSEVMR
                     VLFEKAKRVAATNASVMIYGETGTGKEIIARGIHYHSQRRDESFVPVAVGALPDNLLE
                     GELFGHVKGAFTGANIDKPGLFETADKGTLFLDEMSEVSFDLQHKLLRVLQDHKVRRL
                     GSVKEREIDVRIISATNCDPDVLVKEKRLREDLFYRLQVIRLSIPPLRERREDIPLLV
                     YHFLRQYRQFGPVEVETISSDALLALQQHNWPGNVRQLQNVMEHMVALARKSELDLDD
                     LPEEILPHQKKMFVQVTEDMDFKQAKAAIIKQWEKQYLENLLDKYKTIAKVAKVAGLN
                     RKTIYRLIEAHGIIWPRNSVAAAENGEEEPPDDLEEEEEELLDSEEE"
     gene            3345..3734
                     /locus_tag="PKM23_13125"
     CDS             3345..3734
                     /locus_tag="PKM23_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY92459.1"
                     /translation="MGRTIVVATSDRNLEETLRAQLATDYRVECVRRGADAILQMLEK
                     EIDLLLVDIDMSGQVGTEILPVIRRLRPRLPVILISDDFTYRIRKTAAEQGVTFQSIK
                     PRSSDEALEIAAVMEKIIAHKEGLPVN"
BASE COUNT          794 a         1139 c         1184 g          738 t
ORIGIN      
        1 catccgtctg cagaaagggc tctccggcct gctcctgcac gccctggacc aggccggcgc
       61 ggccggctcc gttcgtctgt ccgtggagcc cgatgcggag ggcggtctgg cgatgcggct
      121 ggtcttcagc ggatcggcgc cgggatggag cgaaccgctc ccctcccagc cgccctggcg
      181 gtcggcccat ctccgcctcg ccctcgctta tgcgatcatt gccgagctgg ggggaggctt
      241 caggatgcgc cggcttcatg acggatcaac ggaaatcgtc attctgctcg ccgcggaggg
      301 caagctgctc tcccggcccg tctgaaggcg ccgccgcagc ccttgctcat gctcccatgc
      361 catgcatcct taagcagagg tttgagtgaa agaaagagta caaaaattta ttgtcgaccg
      421 ccagctgcgc cttcgtcaca gtttcgggga tgtgctggag cggaccctcg gtgagagcct
      481 caagagcggg cagcagctcc atcgccgtct gcaggccctc ggtctgggcg acgcggccga
      541 agccctggcc gaagcggtcc ggcatggccg gagcgccggc ggtgaaaaga tcttcccctc
      601 gccgctgctg gccctgcatt ggctggccga gcccctggtc gagggggaac ggatcgaggg
      661 ggcgaccatc gtgctgacgg atgtcacccg ggagaagcgg cagcagcacc agcaggagtt
      721 gggcggccgg atggaggagg tctcgacctt cgcccacaag ctcgcctccc ggctcaacaa
      781 tccccttgca gctgtcctca acgggatcgg ttgtctggtt ctggaggagg agcccggccc
      841 ggagtggtcc agggtgcgag ccgagctggc cgagctgcag gagcaggtct acgccatctc
      901 ccgagtcacg caagccctgg aatccttcag ccgtgaggag ggaaacagcg gcaagctggt
      961 tcagctcgag gcggtgcttg aaaaggctgt ggaggtcatc cagctgctca ccgctccgcg
     1021 tggggtgcag atcagtgtaa gcgccggtcc tgtctcctca ctcatctatg ccaatgacat
     1081 cctcctcgag cagtgtctgc tccatctgct gcgcaacgcc gtcgaggcct cgccggaggg
     1141 gggagaggtg atcatccgct gcgggagcag tgacggcatg gccgcggtat ccattagcga
     1201 cagtggtgcg ggcattgcgg ccgccgagat gaagaggatt tatgaaccct tttattcgac
     1261 caaatccaca gaccatctgg gtgtgggttt gccgatcagt tacaccatcg ccgcccgcta
     1321 caagggtttc cttgaactgg agagtgtgcc cggcagcggc acgacggcac gtctgctgtt
     1381 tcccgtcgcc aagaacatgg tcaagaaagg atagcacgga atgaatgaca agaagaccag
     1441 tgaccgttcc attctggtca tcgatgatga acgcactgtt ctcgattcac tggtcaagat
     1501 gctcaagaag agccagtaca gctgcattac agccaccaat gccgaagacg gcattcgcct
     1561 tttcgcgcgg gagcgtcccc tgctggttct ttcggacctc tatatgccca atcacaagga
     1621 tggaatccag gtcctggagg aggtgcgccg catcgatccg gatgcggtgg tgatcctcta
     1681 caccgcttac agccacatcc ccgatgtggt cgaggcgacc aagaaggggg cctttgatta
     1741 tatccagaag gttgagaccc accatgacat cctgctgccg atcgaacgcg gcttaaagtt
     1801 tgcgcgcatg cagcgtgaga acgcctatct gcgcagccgt ctcgatctga ctgacgacac
     1861 ccttttttac ggggccatcg ggggcagcga ggtcatgcgc gttcttttcg agaaggccaa
     1921 gcgggtggcg gcgaccaacg cctcagtgat gatctacggc gagaccggca ccggcaagga
     1981 gatcatcgcc cggggcattc attatcacag ccagcgcagg gatgagtcct ttgtgccggt
     2041 cgctgtcggc gccctgcccg acaatctgct cgagggcgag ctgttcggcc atgtcaaggg
     2101 ggccttcacc ggggccaaca tcgacaagcc cgggcttttc gagaccgctg acaaggggac
     2161 gctctttctt gacgagatgt cggaggtgag ctttgacctg cagcacaagc tgctgcgggt
     2221 actgcaggac cacaaggtgc gccggctggg gagcgtcaag gagcgcgaga tcgatgttcg
     2281 catcatcagc gccaccaact gcgatccaga tgtgctggtc aaggagaagc ggctgcggga
     2341 ggatctcttt taccgcctgc aggtcatccg gctgtcgatt ccccccctgc gcgaacgccg
     2401 cgaggacatc cccctgctgg tctaccattt tctgcgccag taccgccagt tcggtccggt
     2461 cgaggtggag acaatttcga gtgatgcact ccttgccctg cagcagcaca actggcctgg
     2521 caatgtgcgc cagctgcaga acgtcatgga gcacatggtc gccctggcgc gcaagagcga
     2581 actcgacctg gatgacctgc cggaggagat tctgccccat cagaaaaaga tgttcgttca
     2641 ggttaccgag gatatggatt tcaagcaggc caaggcagcc atcatcaagc agtgggaaaa
     2701 gcagtacctt gagaatctgc ttgacaaata caagaccatc gccaaggtgg ccaaggtggc
     2761 cgggctgaac cgcaagacca tctaccggct catcgaggcg cacggcatca tctggccgcg
     2821 caacagcgtc gccgccgcgg agaacgggga ggaggagccg cccgatgatc tcgaggaaga
     2881 ggaggaggag ctcctcgaca gcgaggagga gtaatcctgc catgattgac cccgccccga
     2941 agggcggggt tttgtattta ttgtaacaaa gcagatacgc ttacgcccgg gtttgtccat
     3001 tttaagcaac gattagaagt ataataaaat cagtcaaata aaagatagtg tccgtctccg
     3061 gcccctgctc aggtgacatt tctgtggaca gggcggcccc gatcgtctgc ccctccggtt
     3121 acatcctcgt aattgcttaa taaataatac cgaaaaagag cgccgttttt tgaaattctg
     3181 gcacaaaagt tgttcgtttg tctgctgaaa tcagcggcag ccctgcagag gtctgcctcc
     3241 ctgggggtaa agggaggcgg cctctggcag ggagctgcaa cataaaggtg tgggtaacct
     3301 actaggcgaa aggcgcaacg aggattatgc aaacaacgcg caaattgggc agaacgatcg
     3361 tcgtcgccac gagcgatcgg aatctggaag agacgctgcg cgcacaactg gcgacagact
     3421 accgtgtcga atgtgtccgg cgcggtgcgg atgccatcct gcagatgctg gagaaggaga
     3481 ttgatctcct gctggtggac atcgacatga gcggacaggt cggcacggag attctccccg
     3541 tgatccgccg gctgcgcccg cgcctgccgg ttatcctcat ctccgatgat tttacctatc
     3601 gcatccgcaa gaccgcagcc gaacaggggg tgactttcca gagcatcaag ccgcgctcga
     3661 gcgatgaagc cctggaaatc gcagcggtga tggaaaaaat catcgcccac aaagagggcc
     3721 tccccgttaa ctagaatagc gtgacttccg ggttgacggc tgtgcacctg cacccttgtc
     3781 ctcctttcgt tcagccgctc agccgcagcg tattcctcca ccggccactc atctgccgcc
     3841 ggttcccgaa taccg
//
LOCUS       DAOPWU010000329         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_23983_length_3848_cov_5.581070, whole genome shotgun sequence.
ACCESSION   DAOPWU010000329 DAOPWU010000000
VERSION     DAOPWU010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23983_length_3848_cov_5.581070"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..878)
                     /locus_tag="PKM23_13130"
     CDS             complement(54..878)
                     /locus_tag="PKM23_13130"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNY92460.1"
                     /translation="MSDAPKKVIFEVLRYNPGVDARPRMQSYEVPAPKGLTVLDGLLW
                     IKENLDPTLSWRSSCRMGVCGSCAMFINGKPRLACANQVAHLGGGKVVVKPLPNYDII
                     RDLVPDLEPFFAKHAAIKPFLIRKNVEELENPSGEFFQSREELINYIQFAYCIKCGAC
                     LAACPTVATDASFLGPQALAQAWRYTSDSRDEGAAERGKEVFTAHGAFRCHFAGACSD
                     VCPKGVDPAFAIQLMKQFMFREKLGGCKEKHGTEVAPPVENWTPREGIPKPPDHSV"
     gene            complement(875..1240)
                     /locus_tag="PKM23_13135"
     CDS             complement(875..1240)
                     /locus_tag="PKM23_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92461.1"
                     /translation="MRESTLWFWHIIAGAVILVLLGLHMTVMHLDDIIGLYADTTAWE
                     TVIARSQQGFFMVTYILLLGAALYHGLYGFRSILFELSLGKGCQKAINWLFLIGGLLL
                     FIYGAYVAIAVYSMKEVVS"
     gene            complement(1348..1794)
                     /locus_tag="PKM23_13140"
     CDS             complement(1348..1794)
                     /locus_tag="PKM23_13140"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92462.1"
                     /translation="MAETAKHYKNRLGLWGWLGGGRYGLERYAYALHRITGMGILLYF
                     VLHIFVTGSRLGGRESWTKWMGGFESGIFEVGEYLVFIAVAYHALNGIRLALTELGLL
                     MGKPTRPLFPYRSAVLKQRPVLILVMVLAAVLIIAGGLDFFLLGAE"
     gene            complement(1816..3534)
                     /locus_tag="PKM23_13145"
     CDS             complement(1816..3534)
                     /locus_tag="PKM23_13145"
                     /EC_number="1.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013336910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HNY92463.1"
                     /translation="MSKVLTHDLVIFGAGLAGLRAAIEASRQTQGKIDIAVVTKNQMM
                     RAHSVAAEGGTAAVMRTEDGDSLELHAWDTVKGSDFLADQDVVDRFVNAMPGEILQLD
                     HWGIPWTRREDGRIAQRPFGGHSYPRAVLAADKTGFFELQTLYDTLLKYNNTTHYNEF
                     FVTAILTSEGQFRGLAAMNMTTGEFTLIRGKALIICTGGGGTLYGFTTYSQTVTGDGM
                     ALAYRAGLPLEDMEFLQFHPTGLVPSGILITEACRGEGGYLRNSKGERFMEKYAPNKM
                     ELAPRDLISRSEMTEIEEGRGFKGPGGLDYIHLDLTHLGADRINEALPLIREVCMEFN
                     DLEPIDEPIPCRPVAHYSMGGVECDINGATRIKGIWTAGEAACSSLHGANRLGSNSTG
                     ECLVWGQITGAEAARYCATLSEPPALARELLDEEEKRVFGDILGRSGPENLYDLRKEL
                     RATMDKYVGVYRTGPELEKALEIIQGLKKRFANITIPDKSRTYNTNLVHALELQNLID
                     LAEVTVTGALTRTESRGGHARRDFPVRDDDKWLKHTLAFHTPSGPRLDYKSVTITKWK
                     PVERKY"
BASE COUNT          677 a         1186 c         1261 g          724 t
ORIGIN      
        1 tcacaccggg cccctgaatg cgggaaaaat ctgcgcgggc gggagcaaac ccctcaaacc
       61 gaatggtccg gcggtttggg gatgccctcg cgcggggtcc agttctccac cggcggcgcc
      121 acctcggtgc cgtgcttctc cttgcagccg cccagctttt ccctgaacat gaactgcttc
      181 atgagctgga tggcgaaggc gggatcgacc cccttggggc agacatccga gcacgctccg
      241 gcgaaatggc agcggaaggc cccgtgcgcg gtaaagacct ccttgccgcg ttcggccgcc
      301 ccctcgtcgc ggctgtccga ggtgtaacgc caggcctggg ccagggcctg cggaccgaga
      361 aagctcgcgt cggtcgccac ggtcggacag gcggccaggc aggccccgca cttgatgcag
      421 taggcgaact ggatatagtt gatcagttcc tccctgctct gaaaaaactc cccgctcgga
      481 ttctccagct cctcgacatt cttgcggatc aggaagggct tgatcgccgc atgtttggcg
      541 aaaaagggct cgaggtccgg caccaggtcg cggatgatat catagttggg caggggcttg
      601 accaccacct tgccgccgcc gaggtgggcc acctgattgg cgcaggccag gcgcggcttg
      661 ccgttgatga acatcgcgca ggagccgcac acccccatcc ggcacgagga gcgccagctc
      721 agggtggggt cgaggttctc cttgatccag agcaggccgt ccagcaccgt cagccccttg
      781 ggcgccggca cctcgtagct ctgcatgcgc ggcctggcgt ccacgcccgg attataacgc
      841 agcacttcaa agatcacttt tttcggggcg tcgctcatga gaccacctcc ttcatcgaat
      901 agacggcgat ggccacatag gcgccgtaaa tgaagagcag cagtccgccg atgagaaaga
      961 gccagttgat cgccttctgg cagcccttgc ccagactgag ttcgaaaagg atgctgcgga
     1021 agccgtagag gccgtggtag agggcggcgc cgagcagcag gatataggtg accatgaaaa
     1081 agccctgctg gctgcgcgcg atcaccgtct cccaggcggt ggtgtccgca tagaggccga
     1141 tgatatcatc caggtgcatg accgtcatgt ggaggccgag cagcaccagg atcaccgccc
     1201 cggcgatgat gtgccagaac cataaagtgg attcacgcat tcactgttct cctttatccg
     1261 gaaaaatctt ccattctgtt cccggggaaa gccgcctccg ggccggttgc cgcaaacggc
     1321 agcgcccggc cgggcggatt ccgcctccta ttcagccccg agcaagaaga aatcgaggcc
     1381 gccggcgatg atcaggaccg ccgccagcac catcaccaga atcagcaccg ggcgctgttt
     1441 gagcaccgcg ctgcggtagg gaaagagcgg ccgcgtcggc ttgcccatca gcagccccag
     1501 ctctgtcagc gcgaggcgga tgccgttgag ggcgtgatag gcgacggcga tgaagaccag
     1561 gtactcgccc acctcaaaga tcccgctctc aaagccgccc atccatttgg tccacgattc
     1621 ccggccgccg aggcgggagc cggtgacgaa gatatggagg acgaaataga gcagtatccc
     1681 catcccggtg atgcggtgca gggcgtaggc ataacgctcc aggccgtagc gcccgccgcc
     1741 gagccatccc cagaggccga gcctgttttt ataatgtttt gcggtttctg ccataggtca
     1801 atcctcatga aggtcttagt atttgcgttc caccggcttc catttggtga tggtcacgga
     1861 cttgtagtcg agcctcgggc cggagggggt atggaaagcc agggtatgct tgagccactt
     1921 gtcgtcgtcg cgcaccggga aatcgcgccg ggcgtggccg ccgcgggact cggtgcgggt
     1981 cagcgcgccg gtcaccgtta cctcggccag gtcgatgagg ttctgcagct ccagggcgtg
     2041 aacgaggttg gtattgtagg tgcggctctt gtccggaatg gtgatgttgg cgaagcgctt
     2101 cttcagcccc tggatgatct ccagggcctt ttccagctcc ggaccggtgc ggtagacgcc
     2161 gacatacttg tccatggtgg cgcgcagctc cttgcgcagg tcgtagaggt tttcgggtcc
     2221 gctgcggccg aggatatcgc caaagacccg cttctcctcc tcgtcgagca gttcgcgggc
     2281 gagggccggc ggttcgctca gggtggcgca gtatctggcg gcttcggccc cggtgatctg
     2341 gccccagacc aggcattcgc cggtcgagtt ggagccgagg cggttggccc cgtgcaggct
     2401 ggagcaggcg gcttcgccgg cggtccagat ccccttgatg cgggtggcgc cgttgatgtc
     2461 gcactcgacc ccgcccatcg aatagtgggc gaccggccgg caggggatgg gctcatcaat
     2521 gggctcgagg tcattgaact ccatgcagac ctcgcggatc agcggcaggg cctcgttgat
     2581 gcggtcggct ccgaggtggg tgaggtcgag gtggatgtaa tccaggccgc ccgggccctt
     2641 gaatccgcgc ccctcctcga tctcggtcat ctccgagcgc gagatcaggt cgcgcggggc
     2701 cagctccatc ttgttcgggg cgtatttctc cataaagcgc tcgccctttg agtttctcag
     2761 atagccgccc tcgccgcggc aggcctcggt gatgaggatg ccggagggga ccagcccggt
     2821 gggatggaac tggaggaact ccatgtcctc gaggggcagg ccggcgcggt aggccagggc
     2881 catgccgtcg ccggtgaccg tctgcgagta ggtggtgaaa ccgtagaggg tgccgccgcc
     2941 gccggtgcag atgatcaggg ccttgccgcg gatgagggtg aactcgccgg tggtcatgtt
     3001 catcgccgcc aggccgcgga actggccttc ggaggtgaga atggcggtga cgaagaactc
     3061 gttgtagtgg gtggtgttgt tgtacttgag cagggtgtca tagagggttt gcagctcgaa
     3121 aaagccggtc ttgtcggcgg cgaggaccgc gcggggatag ctgtggccgc cgaaggggcg
     3181 ctgggcgatg cggccgtcct cgcggcgggt ccagggaatg ccccagtggt cgagctggag
     3241 gatttcgccg ggcatggcgt tgacaaagcg gtccaccaca tcctgatcgg cgagaaagtc
     3301 gctccccttg acggtatccc aggcgtgcag ctcgaggctg tcgccatcct cggtgcgcat
     3361 caccgcggcg gtgccgccct cggccgcgac cgagtgggcg cgcatcatct ggttcttggt
     3421 gaccaccgca atgtcgatct tgccctgggt ctggcgtgag gcctcgatgg ccgcgcgcag
     3481 cccggccagt cccgctccga aaatgacgag atcatgggtc aaaacttttg acatgcatct
     3541 cctcctctgt tagagctgtg ctgaaatggg aatatagtag tgcggaatga aagatcggcg
     3601 ggttgaagcg atatgaaagc ggctgcttca tgaaggctgg accctaagta gtatgaacca
     3661 aggactggtt caaattagga ttctatttgg attatgtcaa gggaaatctg cagccttttt
     3721 cccgccccca cccgggttcg acgcaccagg tcctccaccc ctcgctctcg cccccacttg
     3781 caccggcctg ctccgccccc cctctcgtcc ctacccgcct ccctctcgcg cccaccctgg
     3841 cccgccag
//
LOCUS       DAOPWU010000330         3808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_24322_length_3808_cov_5.445244, whole genome shotgun sequence.
ACCESSION   DAOPWU010000330 DAOPWU010000000
VERSION     DAOPWU010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24322_length_3808_cov_5.445244"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..601)
                     /gene="tsf"
                     /locus_tag="PKM23_13150"
     CDS             complement(8..601)
                     /gene="tsf"
                     /locus_tag="PKM23_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNY92464.1"
                     /translation="MSITASLVNDLRNKTGAGMMDCKKALEQTGGDIEKAIEYLRKRG
                     VQQAEKKVGRSANQGIIQSYIHPGSRLGVLVEINCETDFVARTPDFQEFAKDIAMQIA
                     AANPLVVDRSELDQQKIDKELEIYRDQAREQGKPEAILDKIAQGKLEKYYQEVCLLEQ
                     SFVKDPNKTIRDLLNEKIAKLGENMILRRFVRFQLGE"
     gene            complement(620..1417)
                     /gene="rpsB"
                     /locus_tag="PKM23_13155"
     CDS             complement(620..1417)
                     /gene="rpsB"
                     /locus_tag="PKM23_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNY92465.1"
                     /translation="MAEVTIQDLLLAGSHFGHLTRRWNPKMKKFIFMERNGIHIIDLK
                     KTIVCLNEAREAMRKIIAEGGTVLLVGTKKQAKDIIKLEADRCGMPYVTERWLGGTLT
                     NFVTVKKSVKRLKNLEKKATDGTYDKVTKKEILTIEREKEKLDKVLGGIRDMNHLPTA
                     LFIVDSKKETIAVQEAKRLNITTFALNDTNADPDFVDYPIPCNDDAFKSISLLTRAIA
                     DAIIEGKSGRIDVQMASESEDNEAEMAYKKEHTKESAESFSSDAVQQ"
     gene            complement(1520..1912)
                     /gene="rpsI"
                     /locus_tag="PKM23_13160"
     CDS             complement(1520..1912)
                     /gene="rpsI"
                     /locus_tag="PKM23_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNY92466.1"
                     /translation="MKGTYYNATGRRKNAIARVWLAPGSGAIEVNEKNLIDYFKRDTL
                     RMIIEQPLEVTGSQGKWDIKAHVEGGGLTGQAGALLLGIARALVKANEELRPMLRKNG
                     FLTRDPRMVERKKYGRPGARKRFQFSKR"
     gene            complement(1948..2376)
                     /gene="rplM"
                     /locus_tag="PKM23_13165"
     CDS             complement(1948..2376)
                     /gene="rplM"
                     /locus_tag="PKM23_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNY92467.1"
                     /translation="MKTYTPKPSDIEQKWYVIDANGQVLGRLACKVAEILRGKNKPIW
                     APHMDCGDFVVIINADKVKLTGRKMEQKKYTHFTGYPGGLRQTPIARMMEKKPEFVVR
                     HAVRGMLPSNTLGRHMLRKLKVYASPEHPHAAQQPEPITL"
     gene            complement(2537..3805)
                     /locus_tag="PKM23_13170"
     CDS             complement(2537..3805)
                     /locus_tag="PKM23_13170"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNY92468.1"
                     /translation="MLLQGGEVLDLASGKRQALDLLILDGRIARTGADLLSDYEGELL
                     DLRGQVIVPGLLDMHVHLREPGREDEETIESGCAAAMAGGITAVCAMPNTEPACDSQE
                     VVRFLKKRAEEQLVEVFPIAAVSKKREGKEITEMAELVRAGAVAFSDDGSPVRSAAVM
                     RRALEYSSMYGVPVIDHCEEPDLAAGGQMNEGWMSTRLGLAGNPDIAEAVMIARDLAL
                     AEFTGGHLHIAHISTALGVELVRRAKETGVKVTCEVTPHHLAFTDEALEHYDTNLKMN
                     PPLRSRADVEALMAGLRDGTIDVIASDHAPHSIEEKEVEFAAAPNGILGLETMLGIIL
                     EKVVAAGVLPLHQALEKMILAPRRVLHLPLPLIKEGVPANLSFFAPEANWSVVAKRLK
                     SLSRNMPYAGWNLPGRVCGVINRGLLWTAE"
BASE COUNT          648 a         1148 c         1134 g          878 t
ORIGIN      
        1 ccgcaggcta ttcgcccagc tggaaccgga cgaaccgtcg caggatcatg ttctcgccca
       61 gtttggcgat cttttcgttg agcagatccc tgatggtctt gttcggatcc ttgacaaagc
      121 tctgttccag caggcagacc tcctggtaat acttttccag tttgccctgg gcgatcttgt
      181 ccaggatggc ctcgggttta ccctgctcgc gcgcctgatc ccggtagatc tcgagttcct
      241 tgtcgatctt ctgctggtcc agctcactgc ggtcgacaac caggggattg gccgccgcaa
      301 tctgcatggc gatatccttg gcgaactcct ggaaatccgg ggtgcgagcg acaaaatcgg
      361 tctcgcagtt gatctcgacg agaacgccca gccggctgcc cggatgaata taggactgga
      421 tgatcccctg attggcgctg cggccgacct tcttctcggc ctgctgcacg ccgcgcttgc
      481 gcagatactc gatcgccttc tcgatgtcgc cgccggtttg ctccagggcc tttttacaat
      541 ccatcatgcc tgcgccggtc ttgttgcgca ggtcatttac taacgaagcg gttatggaca
      601 ttatcgtact cctgactgat cactgttgaa cggcgtcgct ggaaaaactc tcggcactct
      661 cttttgtatg ctctttcttg taggccatct ccgcctcgtt gtcctcgctc tcggaggcca
      721 tctggacatc gatgcgcccc gatttgccct cgatgatggc atcggcgatg gcgcgggtga
      781 gcaggctgat cgacttgaaa gcgtcatcgt tgcacgggat cggataatcg acaaagtcgg
      841 gatccgcgtt ggtgtcgttg agggcgaagg tggtgatgtt cagccgcttg gcctcttgca
      901 ccgcgatcgt ctcctttttg gaatcgacga tgaaaagagc ggtgggcaga tggttcatat
      961 cccggatgcc gccaagcacc ttatccagtt tttccttttc gcgctcgatg gtgaggatct
     1021 ctttcttggt caccttgtcg taggtgccgt cggtcgcctt cttctccaga ttcttcaggc
     1081 gcttgacgct cttcttgacc gtgacgaagt tggtcagggt gccgccgagc cagcgttcgg
     1141 tgacataggg catgccgcag cggtcggctt caagcttgat gatgtccttg gcctgttttt
     1201 tggtgccgac cagcagcacc gtgccgccct cggcgatgat cttgcgcatc gcttcgcggg
     1261 cctcgttcag gcagacaatt gtctttttga gatcgatgat gtggatgccg ttgcgttcca
     1321 taaagatgaa tttcttcatc ttgggattcc agcggcgggt cagatggccg aaatggcttc
     1381 cggccagcag cagatcttga atggtgactt cagccatgat aactcctgat cttggggttg
     1441 aaccgccatc ctccagccca gagatataac cttgacaggc accccttgtt catgggagga
     1501 tgtgtgtgat gagcctggtt taacgtttcg agaactggaa gcgcttgcgg gcgccgggac
     1561 ggccgtactt tttgcgctcg accatgcgcg ggtcgcgggt caggaacccg ttcttgcgca
     1621 gcatcggacg cagctcttcg ttggccttga ccagggcgcg ggcgatgccc agcagcaggg
     1681 cgccggcctg accggtgagc ccgccgcctt cgacgtgcgc cttgatgtcc catttgccct
     1741 ggctgccggt cacttcaagc ggctgctcga tgatcattct gagcgtgtcg cgcttgaaat
     1801 agtcgatgag gttcttctca ttgacctcga tggcgccgct gccaggtgcc agccagacgc
     1861 gcgcgattgc gttcttgcgg cggccggtgg cattgtagta cgtacctttc atttgctctc
     1921 ctttggatct gctcaagcag ggatcgatta cagggtgatg ggttcaggct gctgggcggc
     1981 gtgaggatgc tccggcgaag cgtaaacctt cagcttcctg agcatgtgac gccccagggt
     2041 gttgctcggc agcatgccgc gcacggcatg acgcacaaca aactccggct ttttctccat
     2101 catgcgcgcg ataggcgtct gacgcaaccc gccgggataa ccggtgaaat gggtgtactt
     2161 cttctgctcc atcttgcggc cggtgagctt caccttgtcg gcgttgatga tcacgacaaa
     2221 atcgccacaa tccatgtgcg gagcccaaat gggcttgttc ttgccgcgca gaatctctgc
     2281 gaccttgcag gccagacggc ccaaaacctg cccgttcgca tcgatcacat accatttctg
     2341 ctcgatgtcg ctgggtttcg gggtatacgt tttcaagata tttctccttt attttgcctt
     2401 aagccgagaa atataggcaa attttgcaaa ttatgcaaga tttttctaag gggatggctt
     2461 gttttttaag gaagatatgt caaaatgagg gccggaggcc cgatcagggc ctcgcatcac
     2521 caacggtcat gtgcgctcac tctgcggtcc agagcagccc gcggttgatc accccgcaga
     2581 ccctacccgg caggttccag cccgcgtagg gcatgttgcg ggagagggat ttgagccgtt
     2641 tcgccaccac actccagttc gcctccggag cgaaaaagct caggttggcc gggaccccct
     2701 ccttgatcag aggcagaggc aggtggagca cccggcgtgg agccaggatc atcttctcca
     2761 gggcctggtg cagcggcagt acacccgctg ccaccacttt ttccaggatg ataccgagca
     2821 tggtctccag tccgaggatg ccgttcggcg cagccgcaaa ctcgacctcc ttctcctcga
     2881 tgctgtgcgg cgcatggtcg gaggcgatga cgtcgatggt gccgtcgcgc agcccggcca
     2941 tgagggcctc cacatcggca cggctgcgca gggggggatt catcttgagg ttggtgtcat
     3001 agtgctcgag ggcctcatcg gtgaaggcga gatggtgcgg ggtcacctcg caggtcacct
     3061 tgacccccgt ttctttggcg cggcgcacca gttcgacccc cagggccgtc gagatgtggg
     3121 cgatatggag atgcccgccg gtgaactcgg ccagggcgag gtcgcgggcg atcatcaccg
     3181 cctcggcgat gtccggatta cccgccagac caaggcgggt cgacatccag ccctcgttca
     3241 tctggccgcc ggcggccaga tcgggctcct cgcagtggtc gatcaccggc acgccgtaca
     3301 tgctcgaata ctcgagcgcc cgccgcatca ccgctgcgct cctgaccgga ctgccgtcat
     3361 ccgagaaggc caccgcaccg gctcgcacca gctcggccat ctcggtgatc tccttgccct
     3421 cgcgcttttt gctcaccgct gcaatgggga acacctcgac cagctgctcc tcggcgcgct
     3481 ttttcaggaa acgcaccacc tcctggctgt cgcaggcggg ttcggtgttg ggcatggcgc
     3541 agaccgcggt gatcccgccc gccatggccg ccgcgcagcc gctctcgatc gtctcctcgt
     3601 cctcgcggcc gggttcgcgc aaatggacgt gcatgtcgag cagtccgggc acgattacct
     3661 gaccgcgcag atcgagcagc tcgccctcat agtcggagag cagatcggcg ccggtgcggg
     3721 cgatgcggcc gtcgagtatc agcagatcca gggcctggcg cttcccggag gccagatcga
     3781 gcacctcgcc gccctgaagg aggatttt
//
LOCUS       DAOPWU010000331         3792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_24465_length_3792_cov_2.962269, whole genome shotgun sequence.
ACCESSION   DAOPWU010000331 DAOPWU010000000
VERSION     DAOPWU010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24465_length_3792_cov_2.962269"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(549..2084)
                     /locus_tag="PKM23_13175"
     CDS             complement(549..2084)
                     /locus_tag="PKM23_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNY92469.1"
                     /translation="MLAQILSAAVLGIDAFIVKVEAHLEGGPPYFATVGLPNGAVRES
                     RERVQSAVKNSGFEFPIKHIVINLAPADVKKEGAAFDLPIAMGILAAAGHIRRDNLPE
                     YLILGELSLDGGLRPIRGALPIALSVVQNRLKGIVLPRENASEAAMARDLEIIPVNTL
                     KEAVQFFNRELPIVPFYIDLQEVFANRREYSHDFQDVKGQEHVKRALEVAAAGGHNII
                     MIGPPGSGKTMLAKRLPSILPDLTLAEALETTKIHSVAGLLHPDEALITTRPFRSPHH
                     TISDVGLIGGGNVPKPGEVSLAHHGVLFLDELPEFRKNVIEVMRQPIEEGQVTISRAL
                     TSLTYPAEFMLATAMNPCPCGFYSDPEHNCGCTGQQIKQYLSRISGPLMDRIDIHIEV
                     PAVKYRDLTARPSGEPSAAIRQRVAGARKVQIERFSHYPHLFCNARMDSRELHKYCII
                     DEAGERLLKTAIHKLGLSARAYDRILKVSRTIADLAGSEAIQPLHLSEAIQYRSLDRL
                     QFG"
     gene            2458..>3792
                     /locus_tag="PKM23_13180"
     CDS             2458..>3792
                     /locus_tag="PKM23_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNY92470.1"
                     /translation="MKKYGILLVVALMVCSLFVAPASAQYQAQGWKGGFGIGAIAGNT
                     DLEKSFSDAKLGPQARAFIRYGLGSGLLQGELGINAGVLKGEGYKTHLVPVDFRMLIS
                     PLKLESFNPYLYAGIGSLWYKFKETPAVIAFDDTGDELTQVLPFGLGLQFKVGEKTAL
                     EISGGYNLTMKDNMEYVVAGEDTKDGYYSLLLGLTYGGSEDGDTDKDGLSNKLEKELG
                     TNKKLADSDGDGISDGDEYLKFKTNPLVADSDGDGLSDYDELYKYRTSPVEADTDGDG
                     LTDAQEVQKYLTDPTKADSDGDGLSDSAEINTYKTDPMKADSDGDGLSDGDEVNKYKC
                     NPLLADSDKDGLTDGDEVKKYKTSPMKPDTDGDGLGDYQEVTQYKTDPTKIDTDEGLV
                     ADGIEVNRGTNPLDPNDDLEKEEQIKRAVGTEIILEGIVFDTGSAKIRPSSKV"
BASE COUNT          865 a         1023 c         1099 g          805 t
ORIGIN      
        1 acaatataca tggcggagac gggattgata tcgccgtcga tggtaatttt gagcacctcc
       61 gtctcttctg cagcggcggg cgcaaggccg gcaatcgcag cgatgagcat gaaggcaagt
      121 atgcgcgata ttttcatagg ctttttcctg gttatagcgg aaagggttcc tgatgcaatg
      181 tagccaatgg cggtgagaaa agcaatgctt ttcggccggc ataagacgga cgcagagtgt
      241 aatcagggct gcaaaatggc gcacatttcg cttgactttt gcgcccaatt gtgcaatatt
      301 ggagtaggtg caactcagaa cattgccctg tatgatggta cccggttttt ttgaaccaac
      361 accttcaaaa tgggagctca gctcttaagc gtgtattaca ggcctccgct cttcttcgga
      421 agagcctgcg tggaagtaaa aaatgcttag gcggccccca ccgtaatgaa cgcggttcat
      481 tacagcctgg tactgcgtac agggtttttt attcccctcc ctcctttcct gtccgcctct
      541 cccctttatc aaccgaactg cagcctgtcc aggctgcgat actgtatggc ctcgctcaaa
      601 tggagcggct gaatagcctc gctgccggcc agatcggcga tggtgcgtga gaccttgaga
      661 atgcggtcat aggctcgagc ggagagcccg agtttatgta ttgccgtctt gagcagcctc
      721 tctcccgcct cgtcgatgat gcagtattta tgcagctcgc gggaatccat gcgggcattg
      781 cagaagaggt gcggatagtg gctgaagcgc tcgatctgga cctttctggc cccggctacg
      841 cgctgacgga tggccgcgga gggctcgcct gagggtctgg cggtcagatc gcgatatttg
      901 accgccggaa cctcgatatg gatatcgatg cggtccatca gggggccgga gatgcgcgag
      961 aggtactgct tgatctgctg accggtacaa ccgcagttgt gctccggatc gctataaaag
     1021 ccgcaggggc aggggttcat cgcggtggca agcatgaact ccgcaggata agtgaggctg
     1081 gtgagcgccc ttgaaatggt cacctgcccc tcctcgatgg gctggcgcat cacctcgatg
     1141 acgttcttgc ggaattccgg caattcgtcg agaaagagca cgccatggtg ggcgaggctg
     1201 acctcacccg gtttcggaac attgcctccc ccgatcaggc cgacgtcgct gatggtgtga
     1261 tggggcgaac ggaacgggcg ggtagtgatt agggcctcgt cggggtgcaa gaggccggcc
     1321 acggagtgaa ttttggtcgt ctccagggct tcggccaggg tcagatccgg caggatcgaa
     1381 ggcagccttt tggcgagcat ggttttcccg gatcccggcg gaccgatcat gatgatgttg
     1441 tgtcccccgg cagcggccac ctcaagggcg cgtttgacat gctcctgccc tttcacatcc
     1501 tgaaagtcgt ggctgtattc gcgtcggttg gcaaagactt cctgcagatc aatgtaaaaa
     1561 ggcacaatcg gaagttcgcg attgaagaac tgcacggcct ccttgagggt attcaccggg
     1621 ataatctcga gatcgcgcgc catggccgcc tcgctggcat tttctctcgg caggacgatc
     1681 cccttgaggc ggttctggac gacggagagg gcgatgggca gggcaccccg gatgggccgc
     1741 aggccgccat cgaggctgag ttcgccgaga atcaggtact cgggtaaatt atcgcggcga
     1801 atatgtcccg cggcggcgag gatgcccata gcgatgggca ggtcgaaggc agcgccctcc
     1861 ttcttgacat cggcgggcgc cagattgatg acgatatgct tgatcggaaa ttcaaaacca
     1921 gaattcttga ccgcggactg gacccgttcc cgggattcac ggacagcgcc attggggagc
     1981 ccgaccgtcg cgaaataggg tgggcccccc tccaggtgcg cttccacctt gacgataaaa
     2041 gcgtcgatac ccagaaccgc tgcacttagt atctgcgcga gcatgctgcg actcctgcca
     2101 gtggctgcct ctcccctgct gcgctgccac ctccctccta ttacctaatt taatcgaaac
     2161 cggcaataag tcaagcattt tcctcccccg gtgaaacgct cagggctccc attccattgt
     2221 gaaacaggct tccctgaagc cgccctttta ctctttggga aaggagagca aaatgggccg
     2281 gagtagagag attcctgcag accgtcatgg ttcatcctcc ttggtggttg aagaaggggg
     2341 attgggatat ttcaaacccc gaaaaagtgg gctatttaag ggtatatagg gaatttacaa
     2401 aagataatat tggtttaaaa gagtaaatca acttataaac ccaaggagga aacgagaatg
     2461 aaaaagtacg gcattctgct ggtggtagca ctgatggttt gcagcctgtt cgttgcaccc
     2521 gcttctgcac aatatcaggc ccagggttgg aagggtggtt tcggcatcgg cgcaatcgcc
     2581 ggcaataccg atcttgaaaa atccttttcg gatgccaagc ttggtcccca ggcgcgcgct
     2641 ttcatccgct acggtcttgg ctccggcctt ctgcaaggcg agctgggcat caatgcaggt
     2701 gtcctcaagg gagagggcta caaaacccat ctggtcccgg ttgatttccg catgttgatc
     2761 agccctctca aactggaatc cttcaatcct tacctctatg ccggcatcgg ctccctgtgg
     2821 tacaagttta aggaaacccc tgcggtgatc gcttttgacg acaccggaga tgagttgacc
     2881 caggttctcc ccttcgggct cggcctgcag ttcaaggtgg gcgaaaagac tgccctggaa
     2941 atcagcggcg gctataacct taccatgaag gataacatgg aatatgtagt tgccggcgag
     3001 gacaccaagg atggctatta cagccttctt cttggtctga cctacggcgg ctccgaagac
     3061 ggcgataccg acaaagacgg cctgagcaac aagctcgaaa aggagctcgg tactaacaag
     3121 aagctggccg acagcgatgg cgatggcatc agcgacggcg acgagtacct gaagttcaag
     3181 accaatccgc tcgtggctga tagcgatggc gatggcctca gcgactatga tgagctttac
     3241 aagtatcgca cctccccggt tgaagctgat accgatggcg acggcctgac cgacgcccag
     3301 gaagtgcaga agtacctgac cgatccgacc aaagcggaca gcgacggcga cggcctcagc
     3361 gacagcgccg agatcaatac ctacaagacc gatcccatga aggctgacag cgatggcgac
     3421 ggcctcagcg atggcgatga ggtcaataaa tacaagtgca atcctctcct tgctgattcc
     3481 gacaaggacg gattaacgga tggcgatgag gtcaaaaagt acaaaacctc cccgatgaaa
     3541 ccggatacgg atggcgatgg cctgggcgac taccaggagg tgacgcagta caagaccgac
     3601 ccgaccaaaa ttgataccga tgagggtctg gtcgctgatg gcatcgaagt caatcgcggc
     3661 accaatcccc ttgaccccaa tgatgacctt gaaaaagagg aacagatcaa gcgcgccgtc
     3721 ggcaccgaaa tcatcctcga aggaatcgtc ttcgatactg gcagtgccaa aatccgtccc
     3781 tcctccaagg tg
//
LOCUS       DAOPWU010000332         3769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_24664_length_3769_cov_5.676898, whole genome shotgun sequence.
ACCESSION   DAOPWU010000332 DAOPWU010000000
VERSION     DAOPWU010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24664_length_3769_cov_5.676898"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKM23_13185"
     CDS             <1..281
                     /locus_tag="PKM23_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNY92471.1"
                     /translation="FSSRARAQLLAWRTVTGAGYEIAPTPIVEHNGMVLMPTTAGTVY
                     AIDRKSGEVAWAHKLSEALITGICPAGKRRIVVTALDGTVAAIVWQKR"
     gene            complement(328..1113)
                     /locus_tag="PKM23_13190"
     CDS             complement(328..1113)
                     /locus_tag="PKM23_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNY92472.1"
                     /translation="MKIPLYQVDAFTTKIFAGNPAAVCILEEWLEEPLLQSIAAENNL
                     SETAFLVERDGECEIRWFTPATEVALCGHATLASAYVEFFCRAWPAQSIRFETRQAGP
                     LTVTRRGELLEMNFPARPPQASETPPVLGEILGVKPKQVFTSAEDLLVVVEDETAVLQ
                     ARPDFTALLNFKARGFILTAPGSRCDFVSRFFCPAVGVPEDPVTGSAHCVLIPYWSAM
                     LGKKALHARQLSKRGGELWCRNEGERVTISGRAALYLEGTIVI"
     gene            complement(1110..1847)
                     /locus_tag="PKM23_13195"
     CDS             complement(1110..1847)
                     /locus_tag="PKM23_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNY92473.1"
                     /translation="MNKILDRIREELQQAADEKTRESGLRFFKEAVRLYGVKAAAVEA
                     IGKRYFGELKGMGKEEVLQLCEGLWQSGCMEESFIACQWAYTRHKEFVPDDFATLERW
                     VHTYLSNWASCDTLCNHTIGAFIQSYPEHLADLKRWARTPNRWVRRASAVTLIIPARQ
                     GQFLPDILEIAGILLMDEDDMVQKGYGWMLKAASQAHQQEIFEFVMARKAVMPRTALR
                     YAIEKMPKELKEKAMAREPERRKRAGK"
     gene            complement(1858..>3769)
                     /locus_tag="PKM23_13200"
     CDS             complement(1858..>3769)
                     /locus_tag="PKM23_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92474.1"
                     /translation="NRAWPLVIVMHGYGGNHLEYSGGLQSNPDYFELHILGRARGGWY
                     YDLSEADILDAVDYVRAHWRIDERRIHLNGTSMGGGGTFKLASRYPDRWASGRPICGF
                     GQDQIVRNSLHVPLYSTHSQDDPTVPVLLSRTPLQALLAAGGQAVIDETNGLQHAAWN
                     YHEGNARALNWMTAQVRPDFREVRQIDFTARDRSSCSAYWLKAAEWGSLPGPARFRAV
                     AGRDNQLYLTLDNLSALLIRTRESPLDPLQPLRLSVNGGVPITLSAPLPDSILLRKIP
                     TGWSAAAAGSDTTAFALHTPGGVHNLYQREPLLIVYGTGGSASARQAMAAAALAASKS
                     VHPTWVGDQGDIKDGVPSHHILYGRLKTKPDTAVTAADLERHNLVLIGRAEENQLVQR
                     MAGELPVHFGAEILCSDGLRLPGKGSIMGLYYYNPLVPARLVYWVAAQDPAAYRPGTM
                     ILQLQEETPLGCDLLVLQDDPNRLVKVRHFDSRWRWLTAPEVGEPISATQNSFGQLAA
                     RTAEAMRIAAAADFAIQMVQAPPEQPAGVAGLTTWADFAALYQEAPIGIVQLTGDEIR
                     ALQQALEQSRSPFRFYPQSEGGVIPAKSYRVAMALSFNSMRQLAALLQRVPESLQLSD
                     LTLGQAMQRMLF"
BASE COUNT          671 a         1154 c         1253 g          691 t
ORIGIN      
        1 ctttttcgag ccgcgcgcgg gcgcagctgc tggcgtggcg gacggtcacc ggcgccggct
       61 atgagatcgc gccgaccccg atcgttgagc acaatggcat ggtgttgatg ccgacgacgg
      121 cgggtacggt ttacgccatc gacaggaaga gtggggaggt ggcctgggcc cacaagctct
      181 ccgaggccct gatcaccggc atctgtccgg ctgggaaaag gcggattgtg gtgactgcgc
      241 tcgacggcac ggttgcggcg atcgtctggc aaaagcggtg agcgatcagg agatccgctg
      301 cagttcccga cctgtccatt ggctgtatta aatcactatg gtgccctcga ggtaaagggc
      361 ggcccggccg gagatggtga cgcgctcgcc ctcattccgg caccagagtt cgccgccccg
      421 ttttgagagc tgccgggcgt ggagcgcttt cttgcccagc atcgcggacc agtaggggat
      481 gagcacgcag tgggccgagc cggtcaccgg gtcctcgggc acgccgaccg ccgggcagaa
      541 aaagcgggag acaaaatcac agcgggaacc cggggcggtg aggataaacc cgcgcgcttt
      601 gaaattcaac agggcagtaa agtcgggccg ggcctgcagg acggcggtct cgtcctccac
      661 cacgaccagc agatcctccg cggaggtgaa gacctgtttc ggtttgaccc cgagaatctc
      721 tccgagaacc gggggcgttt ccgaggcctg cggaggccgc gcgggaaagt tcatctccaa
      781 aagctccccg cgcctggtta ccgtcaatgg gccggcttgc cgggtttcga agcggatgct
      841 ctgcgcaggc caggcgcggc aaaaaaactc cacataggcg ctggccaggg tggcgtggcc
      901 gcagagcgcg acctccgtgg cgggggtgaa ccagcggatc tcgcactccc cgtccctctc
      961 caccaggaag gcggtttcgg agaggttgtt ctccgccgcg atcgactgca gcagcggttc
     1021 ctccagccac tcctccagga tgcagactgc ggccggatta ccggcaaaga tttttgttgt
     1081 aaaggcatcg acctgataaa ggggtatttt cattttcctg ctcttttcct gcgctccggc
     1141 tctctggcca tcgccttttc cttcaattcc ttcggcatct tttcaatcgc ataacgcagg
     1201 gcggtgcgcg gcattaccgc cttgcgcgcc atgacaaatt caaaaatctc ctgctgatgg
     1261 gcctggctgg ccgccttgag catccagccg tagcccttct gcaccatgtc gtcctcatcc
     1321 ataagcagta taccggcgat ctccaggata tccggaagaa actggccctg tctggccggg
     1381 atgatcaggg tgacggccga ggcgcgccgc acccagcggt tgggggtgcg ggcccagcgc
     1441 ttgagatcag ccagatgctc cgggtagctc tggatgaaag caccgatggt atggttgcaa
     1501 agggtgtcgc aggaggccca gttgctcaga taggtgtgga cccacctctc cagtgttgca
     1561 aaatcatccg ggacaaattc cttgtgccgg gtgtaggccc actggcaggc gataaagctt
     1621 tcttccatgc agcccgattg ccacagacct tcgcagagct gcaagacctc ctccttgccc
     1681 atgcccttca gttccccgaa atagcgcttg ccgatggcct cgacggcggc ggctttgacg
     1741 ccatagagac ggaccgcctc cttgaagaac cgcaggccgc tctcccgggt cttttcgtcc
     1801 gctgcctgct gcagttcctc cctgatcctg tcgagaatct tgttcatctt ttctccctta
     1861 aaagagcatc cgctgcatcg cctggccaag ggtcagatcg ctcagctgca gcgattccgg
     1921 cacccgctgc agcagagcgg ccagctgccg cattgaatta aaactgaggg ccatggccac
     1981 ccggtaagat ttggccggaa tgaccccacc ttcactctgc ggataaaagc ggaagggcga
     2041 tcggctctgc tccagggcct gctgcagggc ccggatttca tcgccggtca gctgcacgat
     2101 gccgatcggg gcttcctgat agagcgccgc aaaatccgcc caggtggtca ggccggcaac
     2161 accggcaggc tgctccggag gggcctggac catctggatg gcaaaatcag cagcggcggc
     2221 gatgcgcatc gcctcggcgg tgcgggcggc gagctgcccg aagctgttct gagttgctga
     2281 aatcggctcg cccacttccg gagcggtcag ccagcgccag cggctgtcaa agtgacgcac
     2341 cttgaccagg cggttgggat catcctgaag caccagcagg tcgcatccga gcggagtctc
     2401 ttcctgcagc tgcaaaatca ttgtccccgg ccggtaggcg gcggggtctt gcgccgccac
     2461 ccaatagaca agccgcgccg ggacaagggg attgtagtaa tagagcccca tgatgctgcc
     2521 tttacccggc aggcgcaggc catcggagca gagaatctct gcgccgaagt gcaccggcag
     2581 ctccccggcc atgcgctgca ccagctggtt ctcctccgcc cgtccgatca ataccagatt
     2641 atgcctctcc agatcggcgg cggtcaccgc ggtatcaggt ttggttttga gcctcccgta
     2701 gaggatgtga tggctgggca caccgtcctt gatatcgccc tgatcgccca cccaggtggg
     2761 atggacgctc ttgctggcgg cgagagcggc agcggccatg gcctggcgtg cgctcgcgct
     2821 gcccccggtg ccgtagacga tcagcagggg ttcgcgctgg tatagattgt ggacgccgcc
     2881 gggggtgtgg agggcgaagg cggtggtgtc ggatccggcc gcagcagcgg accagcccgt
     2941 cgggatcttc cggagcagga tcgagtcggg caagggggcg gagagggtga tcgggacccc
     3001 tccgttgacg gagagcctca gcggctgcag gggatcgagg ggcgactccc tggtgcggat
     3061 gagcagggcg ctgagattgt ccagggtgag atagagctgg ttgtcgcggc cggcgacggc
     3121 ccggaagcgg gccggccccg gcagagagcc ccactccgct gccttgagcc agtaggcgct
     3181 gcaggaagat cggtctctgg cggtaaagtc gatctgccgg acctcccgga aatcgggtcg
     3241 cacctgggcg gtcatccagt tcagggcgcg tgcattgccc tcgtgatagt tccaggcggc
     3301 gtgctgcagg ccgttggtct cgtcgatgac cgcctgccct ccggccgcca ggagcgcctg
     3361 cagcggagtt cgcgagagca ggaccggcac ggtgggatca tcctggctgt gggttgaata
     3421 gaggggcaca tggaggctgt tgcgcacgat ctggtcctgg ccgaagccgc agatcggccg
     3481 gccggaggcc cagcggtcgg gatagcggga ggccaatttg aaggtgccgc ccccgcccat
     3541 gctggtgccg ttgagatgga tccgccgctc gtcgatgcgc cagtgagcgc gcacatagtc
     3601 cacggcatcg aggatatcgg cctcggagag gtcatagtac cagccgccgc gggcgcggcc
     3661 gaggatgtgc agctcgaaat agtcgggatt gctctgcagg ccgccggaat attcaagatg
     3721 gttgccaccg tagccgtgca tgacgatgac caggggccag gcgcggttg
//
LOCUS       DAOPWU010000333         3747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_24871_length_3747_cov_3.785482, whole genome shotgun sequence.
ACCESSION   DAOPWU010000333 DAOPWU010000000
VERSION     DAOPWU010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24871_length_3747_cov_3.785482"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKM23_13205"
     CDS             complement(<1..439)
                     /locus_tag="PKM23_13205"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNY92475.1"
                     /translation="MLNLILLAAALAFSGGEELPVDRQATPETRALYLNLKKLSGKGV
                     LFGQQDALAYGVGRAHPDSDYCDVRDVCGSYPAVYGWDIGNISASANVDKVHFNKIKR
                     LIKEGYARGGIITVSWHDGNPATGGGVNDTRPSGARLLPGGDAH"
     gene            756..1967
                     /locus_tag="PKM23_13210"
     CDS             756..1967
                     /locus_tag="PKM23_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNY92476.1"
                     /translation="MNTCYQKISSRAKELENEIVEFLLALVATPSHSGVEEKVIQVIR
                     QEMIKAGFDEVRIDGLGSIIGRIGSGKRLIAFDAHIDTVYPGDPAQWNFDPFTPFVRE
                     GKVWGRGSVDQKGGMAAMVYAGRMIREMGLNDKFTILFTGTVMEEDCDGLCWQYLINE
                     EKIRPELVVITEPTNMNIYRGHRGRMEMEVQVKGVSCHGSAPERGDNAIYKAARVALE
                     IEKLNVRLRSDPFLGKGSVTVTEARSSSPSLCAVPDGAALHLDRRLTQGETRESAVAE
                     VHDAAVRAGVPDAQVSVLTYAEPAYTGKIYPTEKYYPTWALPENSPWLQQAAAAYEGL
                     FSRIPLIDKWTFSTNGVAIAGMNNIPCLGLGPGNEVHAHAANEACPVEHLSGAAAFYA
                     ALVAVLNEKAG"
     gene            1969..>2725
                     /locus_tag="PKM23_13215"
     CDS             1969..>2725
                     /locus_tag="PKM23_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNY92477.1"
                     /translation="MSSVFEYQCCECGRCYARDEVTYLCPACSASWKPGMPLKGVLRA
                     EFDYAGIRRRLHPRRIDWRLWNAVEERYFPLCAVGNTPFTRIDRFGTLAGFANLWAKN
                     DGLNPSGSLKDRASFLVAADALRTRARTLVAASTGNAASSLASVCAAAGLKCIIFVPA
                     AAPKAKLVQILMHGATLLPVRGTYDDAFRLSLEFTAKYGGLNRNTAYHPLTIEGKKTA
                     GLEIFQQNRCRVPEVILVPAGDGVIISGVHKAFH"
     assembly_gap    2726..2735
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2736..3220
                     /locus_tag="PKM23_13220"
     CDS             <2736..3220
                     /locus_tag="PKM23_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNY92478.1"
                     /translation="AFYDLRASGLIDKLPRLIAVQAESSDAIHHFIQTGHYRNAEEPH
                     TIADSISVAAPSNAIMARQAVLESGGFSLTVSDEEILAGQKKLAETTGIFAEPAAAAA
                     AAGMLKLKGDARIDPQARIVLLITGHGLKDPGASLGRLQLPQAVEPDLGQVEALLNGE
                     "
     gene            complement(3296..>3747)
                     /locus_tag="PKM23_13225"
     CDS             complement(3296..>3747)
                     /locus_tag="PKM23_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92479.1"
                     /translation="PDFYDLLKLLNDGKVEYLVVSGYAVMYYSEPRFTKDLDIWINPV
                     PENARAVWRALAEYGAPLEKVSLDDFCNLEMIYQIGLAPNRIDILMDVAGVDFAIAWQ
                     RRKVTQYGDVPMNMISREDLIAAKLASGREQDLLDAKGLQKDRPTVD"
BASE COUNT          773 a         1155 c         1104 g          705 t
ORIGIN      
        1 cgtgggcgtc tccgcccggg agcagccggg cgcctgaagg ccgggtgtcg ttgaccccgc
       61 caccggtggc cgggttgcca tcatgccagc tgacggtgat gatcccgccg cgggcatagc
      121 cctcttttat cagccgcttg atcttgttga aatggacctt gtcaacattg gccgaggcgc
      181 tgatattgcc gatatcccag ccgtagaccg cggggtagga gccgcagaca tccctgacgt
      241 cgcagtaatc gctatcggga tgggcgcgtc cgaccccgta ggccagggca tcctgctggc
      301 cgaaaaggac gcccttgccg gagagctttt tcagattgag atagagggcc cgggtctccg
      361 gcgtggcctg gcggtcgact ggcagctcct ctccgcccga gaaggccagg gcggcagcaa
      421 gcagtatcag gtttaacatg gcactctccc gaaatggggg caagtttctt ttaatataat
      481 cagaataacc atgaatatca tcattttaat gcgcagttgt gcataaacgc catgcacaag
      541 cctgagcagt cattttacac agccgacttt cactacgaag caccaccgcc ataatatttc
      601 cgggcagcaa aacggataaa tctcttgctc gccaatccgt aaaaggctat attgaatcca
      661 ggtcatcgaa tgcggggtct ccagggactc cgttttctgg tgtcaagggc agatctgtat
      721 ggtgcatccc caaaaggccg caggagaagc aaaagatgaa tacatgttat caaaaaatca
      781 gctccagagc caaggagctg gaaaatgaga tcgtggagtt tctgctggcg ttggttgcca
      841 ctccctccca ttcgggtgtg gaggaaaagg tcatccaggt catccggcag gagatgataa
      901 aggccggctt cgacgaggtg cgcattgacg gcctcggcag catcatcggc cgcatcggca
      961 gcggaaagcg gctcatcgcc ttcgacgccc atatcgacac cgtctatccc ggggatccgg
     1021 cgcagtggaa ctttgatccc ttcaccccct ttgtccggga gggcaaggtc tggggccgcg
     1081 gcagcgtcga tcaaaagggc ggcatggccg cgatggtata tgccggcagg atgatccggg
     1141 agatgggact gaatgacaag tttaccatcc tcttcaccgg cacggtgatg gaggaggact
     1201 gtgacggcct ctgctggcaa tatctgatca atgaagaaaa gatccggccc gagctggtgg
     1261 tgatcaccga accgaccaac atgaatatct atcgcggcca ccgcggccgc atggagatgg
     1321 aggtgcaggt caagggcgtg agctgccacg gctccgcccc cgagcgcggc gacaatgcca
     1381 tctacaaggc tgcccgcgtc gccctcgaga ttgaaaagct caacgtgcgg ctgcgcagtg
     1441 atccctttct cggcaaaggc agcgtcactg tcaccgaagc gcgctcatcc tctccgtccc
     1501 tctgcgccgt acccgatggc gcggccctcc acctcgaccg ccggctcacc cagggcgaga
     1561 ccagggagag cgcggtcgcg gaggtccatg atgccgccgt gcgggccggc gtgccggatg
     1621 cacaggttag cgtgctgacc tacgccgaac cggcctatac cggaaaaatc taccccactg
     1681 aaaaatacta tcccacctgg gcgctcccgg agaactcgcc ctggctgcag caggcagctg
     1741 cggcttacga ggggcttttc agcaggattc cgctgatcga caagtggact ttcagcacca
     1801 acggcgtcgc aatcgccggg atgaacaaca tcccctgcct cgggctcgga ccgggcaatg
     1861 aggtccatgc ccatgccgcc aacgaggcct gccccgtcga gcacctcagc ggcgccgcgg
     1921 cgttctacgc tgccctggtg gcggtgctca acgaaaaggc cggatgaggt gagttccgtc
     1981 tttgagtacc aatgctgcga gtgcggccgc tgctacgccc gtgacgaggt gacctacctt
     2041 tgcccggcct gctcggcttc ctggaagccg ggcatgcccc tcaaaggtgt gctgcgcgcc
     2101 gagttcgact atgccgggat ccggcgcagg ctgcatccgc gccggattga ctggcggctc
     2161 tggaatgcgg tcgaggagag gtatttcccc ctctgcgcgg tcggaaatac ccccttcacc
     2221 cgcatcgacc gcttcggcac gctggccggg ttcgcaaacc tctgggccaa gaacgacggc
     2281 ctcaacccca gcggctcgct caaggaccgc gcctcctttc tggtcgccgc cgatgccctg
     2341 cgcacccgcg cccgcaccct cgtcgccgcc tcgaccggca acgccgcctc ctccctggcc
     2401 tccgtctgcg cggcggcggg cctgaagtgc atcatcttcg tcccggcggc ggctcccaaa
     2461 gcaaagctgg tccagatcct gatgcacggc gccaccctcc tccccgtcag gggcacctac
     2521 gacgacgcct tccgcctctc actcgaattc acagcaaaat acggcggcct gaaccgcaac
     2581 accgcctatc atcccctcac catcgagggc aaaaaaaccg ccgggctgga gatctttcag
     2641 cagaaccgct gccgtgtgcc ggaggtgatc ctcgtgcctg cgggcgacgg ggtgatcatc
     2701 agcggcgtgc acaaggcgtt ccatgnnnnn nnnnnaggcc ttttacgatc tgagggcgtc
     2761 ggggctgatc gacaaactgc cgcggctgat tgcggtgcag gcggaatctt cggacgccat
     2821 ccatcatttc attcagaccg gccattaccg gaatgccgaa gagccgcaca ccatcgccga
     2881 ttcgatctcg gtcgcggccc cgagcaatgc catcatggca cgccaggcgg tgctggagtc
     2941 gggggggttt tctttgacag tttcagatga ggagattctg gcaggtcaga aaaagctggc
     3001 ggagacgacg gggattttcg ccgagccggc ggcggccgca gcggcggcag gaatgctcaa
     3061 gctgaagggg gacgcacgca tcgatccgca ggcccggatc gtgctgctca tcaccgggca
     3121 cggtctgaag gatcccggcg cgtcactggg ccggctgcag ctcccacagg ccgttgagcc
     3181 ggatctgggt caggttgaag cactgctcaa tggtgaataa tttagccagg agtgcgaagg
     3241 aggaacgaac gaaagagaaa aaaggtggag ggtgagaaat cctgtctgac tcgagttaat
     3301 cgactgtcgg ccgatctttt tgcagaccct ttgcatccag caggtcctgc tccctgccgg
     3361 aagccaattt ggccgcgatc agatcctctc tgctgatcat gttcatgggg acatccccgt
     3421 actgcgttac tttccgccgc tgccaggcga tggcaaaatc aacacccgcc acatccatga
     3481 gaatatcaat ccgattgggc gccagaccga tctggtaaat catctcgaga ttgcagaaat
     3541 catccagcga aaccttttca aggggtgcgc catactcagc caaagccctc caaaccgccc
     3601 tggcattttc cgggacaggg ttgatccaga tatccaggtc tttggtaaag cgcggttcgg
     3661 aataatacat caccgcgtat ccgctaacca caagatactc aaccttgccg tcgttcaata
     3721 gtttcaacag atcgtaaaag tctgggt
//
LOCUS       DAOPWU010000334         3747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_24873_length_3747_cov_3.355634, whole genome shotgun sequence.
ACCESSION   DAOPWU010000334 DAOPWU010000000
VERSION     DAOPWU010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24873_length_3747_cov_3.355634"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKM23_13230"
     CDS             complement(<1..208)
                     /locus_tag="PKM23_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92480.1"
                     /translation="MRYYSAFTRKRAHLLGRLWLAIAVFLLAGAALASAQDNKGIGFH
                     GAPFLRVSSVARAVGMGEAYSVPGA"
     gene            complement(272..3190)
                     /locus_tag="PKM23_13235"
     CDS             complement(272..3190)
                     /locus_tag="PKM23_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92481.1"
                     /translation="MKNGSLFQRHVASAIALLLLLPLSLMAQVTSGDFTDGDPLFTKT
                     IEGPGTMLSDIGTLEPLYASSGEDSTLDYRLSGLEWSYPYRESITAAEDIDSRTLNGQ
                     NLYLITDGLGRRVFEYNANTAVETWSFPPPQSATNPTDEKYLNKPVDAFIYRDNATGY
                     FKVVITDQDRNRVITVDKETSKIDWKYGDPLYREGSGFNQLRQPEDAEKIPNTNEYII
                     ADKGNNRVIIVDGNTNNVIWELGSDVLKSPMDIQYIDGSDHILIADGGNHRVILVDRA
                     SKSILWQFGRTGIADSGAVGLKLPTDADLIPSSQNVIIADAGNERIIEVNPSGQIVWQ
                     YHRRLKGLRDADRIEEGRTLAVFENYPYRIAYTEAFVVSGKYDLGELHASVFDSLFWT
                     ADTVAGVTSAWFQLRTAESDFALDGATWYGPTGKESYYTFSGCALNQVHTGGRWYQFR
                     VLLRTSDPLQTPIIRQVVVKHHYYQVNQPNTYFYTPIITEEAGKLISQWNRLTFRTIL
                     PKEVEKRAAVDIEVQILNGKNSEVLERFTASKLNESNEITLSSLASLRGIQSIALVAK
                     LSTGNSSMTPIMDNWRVTWDAIPTANSSIAFTDKNANAKEYYRATTTVPSSENLVDSL
                     YILLRDPDLEPFKQTHRVTVHALGTRDSVNVDLKLLTLGGFFSAKAIPILINSAAVKG
                     NNIMEVQDRDYLVVGYQDSLTALDTASDTIQVVKNSAGAMTIENARKVELTKAWFGDT
                     LYIRIKNETDHNLDPDLAETLHISVFDNVTQDKEDVILTELPSPTTGRFNTGEFFSQT
                     GLVVNRNNNGVRGNGQIETQPGHTVAAEYIDNLTLTRYVLIPVESDTSARDSIYIFYG
                     RRPDGVEIGPNPYSPRRDRKFRMRVGFSTDSLAVTSIEIFNLAGEKVRTLLAGRDINF
                     VTENNVSLNSSDMWWDMRNESGQEVASGTYWAKVNAELRAATTLSRQVNFIRKFVIVR
                     "
     gene            complement(3357..>3747)
                     /locus_tag="PKM23_13240"
     CDS             complement(3357..>3747)
                     /locus_tag="PKM23_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNY92482.1"
                     /translation="RVAIIAAVEKVNHDSMPCTVDAAALAQMGARGQIKGAIIDGPLA
                     VDNAVCRKACEVKGISSEVGGEADILIMPDIEAANVFYKTMTYLGGSRTAGIIVGAQC
                     PVILTSRADSEETKFYSIALAMATGAR"
BASE COUNT          753 a          960 c         1179 g          855 t
ORIGIN      
        1 cagcgccggg gacggagtaa gcctccccca tgccgactgc acgtgccacc gagctgacgc
       61 gcaggaaggg tgcgccatga aaaccgattc ccttgttatc ctgcgcggat gcgagagcgg
      121 cgcctgcgag caggaacacc gcaatcgcga gccagaggcg gccgagaaga tgcgcccgtt
      181 tgcgtgtaaa ggctgaatag tatctcattt tagctccatt gccaagtcga tgaatagaat
      241 gaaccgccat atttagggca agcgcattgt tctagcgtac gatgacaaac ttgcggatga
      301 aattgacctg ccgtgaaagc gttgtggcag cacgcagttc cgcattgact ttggcccagt
      361 aggtcccgct tgcgacctcc tgtccgctct cgttgcgcat atcccaccac atgtcactgc
      421 tgttcagact gacattgttt tcagtgacga aattgatatc tcttcccgcc agcagggtgc
      481 gcactttttc gcctgccaga ttgaagattt cgatagaggt gaccgcaagc gaatcggtgc
      541 tgaagccgac acgcatcctg aacttgcggt cccggcgcgg cgagtaggga ttgggtccga
      601 tctccacacc gtcgggtctg cggccgtaaa aaatgtagat gctgtcgcgt gcgctggtgt
      661 cactctcaac cgggatgaga acgtatcggg tcagggtcag attgtcgatg tattccgctg
      721 ctacggtgtg gccgggctgg gtctcgatct gaccgttgcc gcgaacgccg ttattgttgc
      781 ggttgacgac caggccggtc tgggagaaga actctccggt attaaaacgg ccggttgtgg
      841 gcgagggaag ctcggtcagg atcacatctt ccttgtcctg ggtgacattg tcgaacaccg
      901 agatgtgcaa ggtttccgcg aggtcgggat cgaggttatg atcggtttcg tttttaatgc
      961 ggatgtagag ggtgtctccg aaccatgcct tggtgagttc aaccttgcgg gcgttttcta
     1021 tggtcatcgc cccagccgag tttttaacca cctggatggt gtcgctggcg gtatcgagtg
     1081 cggtgagcga gtcctggtag ccgaccacga ggtaatcgcg gtcctggact tccatgatat
     1141 tgttgccctt gaccgctgca gagttgatca ggatagggat ggctttggct gagaaaaagc
     1201 ccccgagcgt caacagcttg agatcgacgt tgaccgaatc gcgggtgccg agggcgtgga
     1261 cggtgacacg gtgggtctgc ttgaagggtt cgagatccgg atcccgcagc aggatataaa
     1321 gactgtcgac caggttctcg ctggaaggca ccgtggtcgt ggcgcggtag tactccttcg
     1381 cattggcatt tttatccgta aaggcaatgg aggagttggc cgtcgggatg gcatcccagg
     1441 tgaccctcca attgtccatg atcggagtca tcgaggagtt gccagttgag agtttggcga
     1501 cgagggcaat ggactggatg cccctgagcg aggccagcga ggagagggtg atctcgttgg
     1561 actcgttaag tttgctcgcc gtgaagcgtt cgagcacctc cgagtttttg ccattaagga
     1621 tttggacctc gatgtcgacc gcggcccgct tttccacctc tttgggcaga atggtcctga
     1681 aggtgagccg gttccactgc gagatcagct tcccggcctc ctcggtgata atcggggtat
     1741 agaaataggt gttgggctga ttcacctgat aataatgatg tttcaccaca acctgcctga
     1801 tgatcggggt ctggagggga tcgctggtcc tgagcaggac gcggaactgg taccagcgtc
     1861 cgccggtgtg gacctggttg agggcgcagc ccgaaaaggt gtaatagctt tccttgccag
     1921 tcgggccgta ccaggtggcg ccgtcgagtg caaaatcgga ctctgctgtc cgcagctgga
     1981 accaggcgga ggtgacgccg gcgacggtgt cggctgtcca gaagagggaa tcgaagacag
     2041 aagcgtgcaa ttcgccgagg tcgtattttc cggagaccac aaaggcttcg gtgtaggcga
     2101 tgcggtaggg ataattctca aagacggcga gggtgcgtcc ctcctcgatc cggtcggcgt
     2161 cgcgaagccc tttcaggcgg cggtggtact gccagacgat ctgaccggag gggttgacct
     2221 cgataatgcg ttcattgccg gcatctgcga tgatgacatt ctgagaggag ggaatcagat
     2281 cggcatcggt cggaagtttc agtccgacag cgccgctgtc ggcgatgccg gtgcgtccga
     2341 actgccagag gatcgatttg gaggcgcgat cgacgaggat gacgcggtgg ttgccaccgt
     2401 cggcgatgag gatgtgatcg ctgccatcga tatactggat gtccatcggt gatttgagca
     2461 catccgagcc caattcccag atgacattgt tggtgttgcc gtcaacaatg atgacgcgat
     2521 tattgccctt gtcggcgatg atatactcat tggtgttggg gattttttcg gcatcctctg
     2581 gctgacgcag ctgattgaag ccgcttcctt cccggtagag gggatccccg tatttccagt
     2641 cgattttgct ggtctccttg tccaccgtga tgacccggtt gcggtcctgg tcggtgatca
     2701 ccaccttgaa atagccggtg gcgttgtccc gatagataaa ggcatcgacc ggcttgttga
     2761 ggtatttctc gtcggtcgga ttggtggcgc tttgcggagg tggaaaggac caggtctcga
     2821 cggcggtatt ggcgttgtat tcgaaaaccc gcctgcccaa accatcggtg atgagataga
     2881 gattctggcc attaagggtg cggctgtcga tgtcctcggc agcggtgatc gattcgcgat
     2941 agggatagct ccactccagg ccggagaggc gataatcgag tgtcgaatcc tcgccggatg
     3001 aggcatagag gggttccaat gtgccgatat cgctcagcat ggtgcccgga ccctcgatgg
     3061 ttttggtgaa aaggggatcg ccatccgtaa aatcgccgct tgtaacctgt gccataagtg
     3121 aaagaggcag cagcaggagg agggcgatgg ccgacgcgac atggcgttga aagagagagc
     3181 catttttcat gattcattcc gtccttttaa tccgaaatga tgcggttgat aattaattat
     3241 ttactggagc agtcaaaaaa agtacgtctg gtagagcggg ctcgcaagcc catatgccac
     3301 tgctgcggct ggtggtcaga ccggcggcgg gagaaccatc cggcgccggc ggatgatcat
     3361 cttgcacccg tggccatggc cagagcgatg gaatagaatt tggtctcctc gctgtccgcc
     3421 cggctggtca ggatgaccgg gcactgcgcg ccgacgatga tcccggccgt tctggaacct
     3481 ccgagatagg tcatggtttt gtaaaataca ttggcagcct caatatccgg catgatcagg
     3541 atatcggcct cacctcctac ctcgctcgag atccccttga cttcgcaggc cttccggcag
     3601 acggcattat cgacggccag ggggccgtca ataatggcac ccttgatttg gccgcgagca
     3661 cccatctgag cgagagcggc ggcatccacc gtgcagggca tggagtcgtg attgaccttt
     3721 tccaccgccg cgataatcgc cacccgg
//
LOCUS       DAOPWU010000335         3647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_25820_length_3647_cov_3.818486, whole genome shotgun sequence.
ACCESSION   DAOPWU010000335 DAOPWU010000000
VERSION     DAOPWU010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25820_length_3647_cov_3.818486"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..>930
                     /locus_tag="PKM23_13245"
     CDS             143..>930
                     /locus_tag="PKM23_13245"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNY92483.1"
                     /translation="MCKFLRTLLPLALLHPIALQAQIWDKSFGVTLQTGLVKFLGDTP
                     DRAAVGNATGLGLKYGLSRHLMLQGELGYGSFKPSKEGSRWQKDPEVMYRTFLFPLTV
                     GARITPSRNGAVKPYLTVGTGILFWDLRYLSGEKLTFWSDHYWRWGDRVSGWRRNGLL
                     YEGLGLELYPHPALSIDLQARFSSLLHLRRDNVGQDDINSQYLQAMATLTWYISYQRD
                     RDRDGIVDRFDADPLHAEDLDGFQDLDGAPDPDNDNDGVPDVRDP"
     assembly_gap    931..940
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <941..1768
                     /locus_tag="PKM23_13250"
     CDS             <941..1768
                     /locus_tag="PKM23_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNY92484.1"
                     /translation="PAPLIPEDLDGFQDDDGIPDPDNDLDGIPDAGDLCINAPEDFDH
                     FEDDDGCPDPDNDQDGIEDAIDACPGKAEDHDGFEDRDGCPDIDNDGDLIVDLVDKCP
                     DQPETVNGYLDEDGCPDSDLDGDGIPDELDRCPRDPETRNGFEDEDGCPDDKQLAPAA
                     KSGAALILQGVNFASGKAELTAESLPVLDEVANSLLLEPAAVVEIRGHTDNVGKAAAN
                     QLLSERRAESVRRYLISKGVSGERITAIGFGQRYPIADNKTPAGRAQNRRIEFVRIK"
     gene            complement(1856..2497)
                     /locus_tag="PKM23_13255"
     CDS             complement(1856..2497)
                     /locus_tag="PKM23_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92485.1"
                     /translation="MKTTTLTLSLGLLLAITPGCDSSINKSIYVQDGERRDSGINTVN
                     GTITIGNDCVIRGDCRTVNGAIRVGQNSIVRELQVVNGEIELGRKSRSKNDLMTVNGT
                     ITLQPGSLVNGEVSTINGGIRMDNATVEQDLTLFNGNIELVNASVVKGDIIIKDSKGN
                     RDRNQVYTIRLGEGSVVEGDIFVQEDEVKVKVVLGKDASVKGEVTNAEVSREL"
     gene            complement(2573..>3647)
                     /locus_tag="PKM23_13260"
     CDS             complement(2573..>3647)
                     /locus_tag="PKM23_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNY92486.1"
                     /translation="AMKQDGYDPSLIHGCHNRTVVIDAHLGERENKQVHVVEAFGLRN
                     IHLYEDEEWIDVRTAVGDLTDRFLCLNEVYRDGFMIPKRLIGENIIHLPTIKTHIFTT
                     TTGAMKNAFGGLLNERRHWTHPVIHETLVDLLRIQQKIHTGIFAVMDGTFAGDGPGPR
                     CMTPHVKDLLLASSDMVAIDAISAKLMGFDPLSIKYIRLAHEAGLGCGDPREIEIVGD
                     EEAGRENWHFVGPFKKMTFASRMQHRIYWGSLKKPIEWSLKTVLAPWSYIASVTYHDS
                     FWYPLFARKKMAAALSSHWGRLFRNYETLQATERGFETIGTESAPFKRTGWRAAVRSI
                     PILFKSIKEAPEFIQRRKRRKKK"
BASE COUNT          688 a         1089 c         1102 g          758 t
ORIGIN      
        1 cttgtaaagt tatcggcttc cggagaaccg agctgccacc gggagggagt atttacgcat
       61 tcgtctgttt tttttgtact atgatgtaca gcaacgatgc aatccttctt cgtgactcca
      121 cctactagtt tcggggctcg gcatgtgcaa atttcttcgg acactcctcc cgcttgctct
      181 tttacatccc atcgccctgc aggcgcagat ctgggataaa agcttcggcg tcaccctcca
      241 gactggtctg gtcaaatttc tcggtgacac gcccgatcgc gccgcggtgg gcaatgccac
      301 agggctgggc ctcaaatacg gcctcagccg ccacctcatg ctgcagggag aactgggcta
      361 cggcagcttc aaaccgagca aggaaggatc ccggtggcag aaagatcccg aagtgatgta
      421 ccgcaccttt ctcttcccac tgaccgttgg ggcacggatc actccgagca ggaacggcgc
      481 ggtcaaaccc tacctcacgg tcggcaccgg catcctcttc tgggacctgc gttacctgag
      541 cggagaaaag ctgaccttct ggagcgatca ttactggcgc tggggtgacc gcgtcagcgg
      601 atggcgcagg aatggcctcc tctacgaggg gctcggcctc gaactctatc ctcatccggc
      661 tctatccatc gacctgcagg cgcgcttcag ttctcttctc cacctccgcc gcgacaacgt
      721 cggccaggat gacatcaatt cccagtacct gcaggcgatg gccaccctca cctggtacat
      781 cagttatcag cgggaccgcg accgggacgg catcgtcgac cgctttgacg ctgatcccct
      841 gcacgccgag gatctggacg gctttcagga cctcgacggc gcacctgatc ccgataacga
      901 caatgacggg gttcccgatg tgagggatcc nnnnnnnnnn ccggcccccc tgattccgga
      961 ggatctcgac ggcttccagg atgacgacgg cattcccgat cctgacaacg acctggatgg
     1021 catccccgat gccggggacc tctgcatcaa tgcccccgag gatttcgacc atttcgaaga
     1081 tgatgatggc tgccccgacc ccgacaacga tcaggacggc atcgaggacg ccatcgacgc
     1141 ctgccccggc aaggccgagg accatgatgg ctttgaggac cgggacggct gccccgatat
     1201 cgataatgac ggtgatctca tcgtcgatct ggttgacaag tgccccgacc agcccgagac
     1261 cgtcaatggc tatttggatg aggatggctg ccccgattcc gatctggacg gggacggcat
     1321 ccccgacgag ctcgaccggt gtccgcgcga ccccgagacc aggaacggct ttgaggatga
     1381 ggacggctgc ccggacgaca aacagctcgc ccccgccgca aagagcggtg cggccctgat
     1441 tctgcagggg gtgaatttcg cctcgggcaa ggcggagctg accgccgaat ccctgccggt
     1501 gctcgacgag gtcgccaact ctctgctcct cgaacccgct gctgtcgtcg aaatccgcgg
     1561 ccatactgat aacgtcggca aggcggccgc caaccaactg ctatcggagc gccgggccga
     1621 atcggttcgc cgctacctga tcagcaaggg ggtgagcggt gaacgcatca ccgcaatcgg
     1681 cttcggccag cgttacccca tcgcggacaa taaaaccccc gcggggcgcg cgcaaaaccg
     1741 ccgcatcgag ttcgtcagga tcaaataggg aggatccaac gacaagaaaa aagcctgccc
     1801 cgctgtgccg gggcaggctt ttttttatgc tttccatata aaagcggcgg cgggatcaga
     1861 gctcccgact gacctcggcg ttggtgactt ctcccttgac actggcatcc ttgccgagca
     1921 ccaccttgac cttgacctca tcctcctgca cgaagatatc cccttcgact acggagccct
     1981 cgcccagccg gatggtgtac acctgattcc ggtcgcggtt gcctttcgaa tccttgatga
     2041 tgatgtcacc cttgaccaca gaggcgttga cgagttcgat attgccgttg aagagggtca
     2101 aatcctgttc gacggtggca ttgtccatgc ggatgccccc gttgatggtg gagacctccc
     2161 cgttgaccag gctgccgggc tggagggtga tggtgccgtt cacggtcatg aggtcattct
     2221 tggacctcga tttccggccc agttcgatct cgccgttgac cacctggagt tcgcggacaa
     2281 tggaattctg tccgacacga atggcgccgt tgacggtgcg gcagtccccg cggatcacgc
     2341 agtcattgcc gatggtgatg gtgccgttga ctgtattgat gccgctgtcg cggcgttcac
     2401 cgtcctgaac gtagatgctc ttgttgatgg acgaatcgca tcccggcgtg atcgccagga
     2461 gcaggcccag gctgagagtc agagtggtcg ttttcatcac gtagccctca caataaatgg
     2521 tgacagtgtg tggcttgcct aatcctgttt acgtgacgaa ttcccggggg tttcactttt
     2581 ttttacgtct tttcctgcgc tggatgaact cgggagcctc cttgatgctc ttgaagagga
     2641 tgggaatcga gcgcacagcg gcgcgccagc cggtgcgttt gaatggggcc gattccgtcc
     2701 cgatagtctc gaagcccctt tcagtggcct gcagggtctc atagttgcgg aaaagccggc
     2761 cccagtgcga gctcagggcg gccgccatct ttttcctggc gaataagggg taccagaagc
     2821 tgtcgtgata ggtcaccgag gcgatatagg accagggggc cagcacggtc ttgaggctcc
     2881 attcgatggg ctttttcagg gagccccagt agatgcgatg ctgcatgcgc gaggcgaagg
     2941 tcatcttttt aaaggggccg acgaagtgcc agttttcgcg gcccgcctcc tcgtccccga
     3001 cgatctcgat ctcgcgcgga tcgccgcagc ccagcccggc ttcatgcgcg aggcggatat
     3061 acttgatcga gaggggatcg aagcccatca gtttggctga aatggcgtcg atggcgacca
     3121 tatccgaact ggcgagaagg agatccttga cgtggggggt catgcagcgc gggccggggc
     3181 cgtccccggc gaaggtgcca tccatcactg cgaaaatccc ggtgtggatt ttttgctgga
     3241 tgcgcagcag gtcgacgagg gtttcgtgga tgaccggatg ggtccagtga cgccgctcgt
     3301 taagaagccc cccgaaggcg ttcttcatcg ccccggtcgt ggtggtgaag atatgggtct
     3361 tgatggtggg gaggtggatg atgttctcgc cgatgagccg tttggggatc atgaagccgt
     3421 cccggtagac ctcattcagg cagaggaacc ggtcggtcag atcgccgact gcggtgcgga
     3481 cgtcgatcca ctcctcgtcc tcgtagaggt ggatgttgcg caggccgaag gcctcgacca
     3541 catggacctg tttgttctcg cgctcaccga ggtgggcgtc gatgaccacg gtgcggttgt
     3601 ggcaaccgtg gatcagggac gggtcgtagc cgtcctgctt catggcg
//
LOCUS       DAOPWU010000336         3645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_25836_length_3645_cov_4.623120, whole genome shotgun sequence.
ACCESSION   DAOPWU010000336 DAOPWU010000000
VERSION     DAOPWU010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25836_length_3645_cov_4.623120"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PKM23_13265"
     CDS             complement(<1..979)
                     /locus_tag="PKM23_13265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNY92487.1"
                     /translation="MNRIPGDEIKSVLVSRLRFMGDVILTTPLLAALRRGFPGVHIGY
                     LVEEPYHALLEHHPDVDELYAFKRGSDRGQLDLVRALRRRKWDASIDLFGNPRSALLL
                     YMSGARWRIGGDFRGRRHLYTHRIPDHGERMTAIAYHLRYLAPLGIEGGGDPPRIVVT
                     EAEKEEARRYLQSKGYDPGRPVIGLHPGATWPAKRWFPERFAALAARLLNEKRQVLFT
                     MGPGEEEIVAGVLGLLPAGLPPPEILPLRRLAALLSQLALFVSNDCGPLHLAPAVGTR
                     TVGIFGPGEPDIWFPYAAAAGHRLAYHSLDCSHCHRDHCSSMACMRAISV"
     gene            complement(990..1805)
                     /locus_tag="PKM23_13270"
     CDS             complement(990..1805)
                     /locus_tag="PKM23_13270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY92488.1"
                     /translation="MAADGDTAVRYALYRFRSLEPGAADLADVRNLLLVTGRTEAEVA
                     PASLNGEYAFAVTALDRNANEGPPSNIVVVAPPPQPLLAWPLPHRDDVGDSVTLRWQD
                     LNLVSHYEVQLGLDSSFAAPLVLHHPAVRDTFLVVAGLRPGLTYYWHVRAANPAGAGL
                     FSTTGLFRCKESQTTAVAELATAPALFELQQNFPNPFNPVTLISYQLPGDGWVRLTVH
                     DLLGRTVAELVNGQRGAGRHVVSFNGADLANGTYLYRLHYEGRILNRRMTLLK"
     gene            complement(2103..2987)
                     /locus_tag="PKM23_13275"
                     /pseudo
     CDS             complement(2103..2987)
                     /locus_tag="PKM23_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
     gene            complement(3105..>3645)
                     /locus_tag="PKM23_13280"
     CDS             complement(3105..>3645)
                     /locus_tag="PKM23_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY92489.1"
                     /translation="VAKNTLPEVTQWVKVDWYGWRLVEWKLSDPGVVGAWLATNNQLD
                     GSKLSIDSIQLMKTADSAVSGAIFVDNLVVVTRTSVPTTVEMPAETLPARLALEQNYP
                     NPFNPVTTIAFDLPAAAQIRLAVYDLLGREVAVLADGLQPAGRHRIAVDARSWGSGTY
                     LYRLTRDGQALTRKMLLIK"
BASE COUNT          694 a         1085 c         1241 g          625 t
ORIGIN      
        1 ccaccgagat cgctcgcatg caggccatgg agctgcagtg atcgcgatgg cagtggctgc
       61 agtcgaggga gtgataggcg aggcggtggc ccgcggccgc agcatagggg aaccagatat
      121 cgggctcgcc cgggccgaag attcccaccg tcctggtccc caccgccggt gcgagatgga
      181 gcggtccgca gtcattgctg acaaagaggg cgagctggct gagcagtgcg gccaggcggc
      241 gcaggggcag tatttccgga ggcggcagcc cggccggcag caggccgagc acacctgcga
      301 cgatctcctc ctccccgggc cccatggtga agagaacctg ccgcttctcg ttgagcaggc
      361 gggcggcgag ggcggcaaag cgctcgggaa accagcgttt tgccggccag gttgcgccgg
      421 gatgcagccc gatgaccggc cggccgggat cgtatccctt ggactgcaga tagcggcgcg
      481 cttcctcttt ctccgcctcg gtcaccacga tgcggggcgg atcccccccg ccttcgatcc
      541 ccaggggggc gagatagcgc agatggtagg cgatggcggt catgcgctcc ccgtggtccg
      601 ggatgcgatg ggtgtagaga tggcggcggc cgcgaaaatc gccaccgatg cgccagcgcg
      661 ccccactcat atagagtaaa agggcggaac ggggattgcc gaagagatcg atcgacgcat
      721 cccatttccg gcgccgcagg gcgcgcacca ggtcaagctg gccgcggtcg ctgccgcgct
      781 tgaaggcgta gagctcatcc acatcgggat gatgctcgag cagggcatga tagggttcct
      841 cgaccagata gccgatgtga acccccggga accctctgcg cagggcggcg agcaggggcg
      901 tggtcagaat gacatcgccc atgaagcgca gccgcgacac cagcacggac ttgatctcgt
      961 cgcccgggat cctgttcatg atcggtccct catttcaaca gcgtcatcct gcgattgagt
     1021 atccggccct cataatggag gcgataaaga taggtgccat tggccagatc cgcgccattg
     1081 aaagagacca catgccggcc ggctcctcgc tggccgttca ccagttcggc cacagtacgt
     1141 ccgagcagat cgtgcaccgt caggcgcacc cagccatcac ccggcagctg gtaggaaatc
     1201 agggtgaccg gattgaaggg attgggaaaa ttttgctgca gttcaaagag agcaggggcg
     1261 gttgccaact ccgccacggc cgtggtctgg ctctccttgc agcggaaaag cccggttgtt
     1321 gagaagagtc cagcgcccgc cggattggcg gcccgcacat gccagtaata ggtcaggccc
     1381 ggccgcagcc cggcaaccac caaaaaggta tccctgacgg ccggatggtg caggacgagt
     1441 ggcgcggcga aagaagaatc gaggccgagc tgcacctcat aatggctgac cagattcaga
     1501 tcctgccagc gcagggtcac gctgtcgccg acgtcgtcgc ggtgcggcag cggccaggcg
     1561 agcagcggct ggggcggcgg tgcgacgacg acgatgttgc ttggcgggcc ctcattggcg
     1621 ttgcggtcga gtgcggtgac cgcaaatgca tactcgccat tcagggaggc cggcgccacc
     1681 tccgcttcgg ttctgccggt gaccaggagc aggttacgca catctgccag gtcggctgct
     1741 cccggttcga gcgatctgaa gcggtagagg gcgtagcgca ccgccgtatc tccatcagcg
     1801 gccatggcgg gcgggtccca atggattttg gccggctttc ccgcctccca ctggtcaaaa
     1861 cagagattga agggggaatt ggggggcagc actcccttcc aggccatcac gggaggcagg
     1921 gcgggaaaag cgtagagatc cctgcgcagg ctgtcgccaa gacctttggt attgttttgc
     1981 acaaagcggg cggaatagaa gacattgccg ccgatgctgg tgatggcgcg attgaagcgc
     2041 acctgatcgc agacctcgga ggccggccac tgcccggagg gaaaggccgc agtggccgtc
     2101 atcttgtaaa tgccctggcc gatgtaaaga tggatgccct ccatcaccga ggcccaccac
     2161 ggcgccagcg ccccgtaggc ggcgatgcgc aggccaaagg accagtagat ctgcggggtg
     2221 aggtaatcga tgcttttgtc ccgaatccag gccaggggat cgcagtagat ggagtaatag
     2281 gattccatgc cggccgtgcc ggcatcgctg ttcttgcgga tgccgaaggg actgatgccc
     2341 agcttgacgt gcggtttgac ggcgatcagg ctgtcgtgca gggtgcggac aaagaggttg
     2401 atattgtccc gccgccagtc gccgcgctcg gtaaagccgc ggggataggc cgcaaaactg
     2461 gcatcatcct cgttggtgat tccctcgtag ggatagaaat agtcatcaaa gtgaacgccg
     2521 tcgaggtcgt agcgccgcac cacgtcaagg atgacggaga ggacatactc ccgcacctgg
     2581 ggcagaccgg ggtcaagata gcggtagttc cccttgttca ggatccacgc gggatgggtg
     2641 ttcatgacat ggttggcagc caacgggtag gcattgacgc tgcgctgcgc ccggtaggga
     2701 ttgaaccagg cgtgcacctc catcccgcgc cggtgcgcct cggcgagcag aaaggcaagg
     2761 ggatcataaa agggcgacgg tgcgcggccc tgggcgccgg tgagatggta ggaccatggc
     2821 tcaagggatg attgataaag ggcatcacac tccggccgga cctgaaagat gacaccattc
     2881 atccccgccg ctttcaggtc atcgagcagg cgcagcagct cggttttttg cgcttcgctg
     2941 ccattggttt tgggccagtc gatgcgggtg acggtggcga tccaggcggc gcgaaactcc
     3001 cgtttcgggg gagctccgcg gcggcagcac cgcggagcag cccgagaaaa accagtacaa
     3061 gaaaaccgat tctgcgcatc gctctttccc tggccttggc gtctttattt gatgagcagc
     3121 atcttgcggg tcagggcctg gccgtcgcgg gtgaggcgat agagataggt gcccgaaccc
     3181 caggaccggg catccaccgc gatgcggtgg cgaccggcgg gctgcaggcc gtcggcgagc
     3241 acggccactt cgcgcccgag cagatcatag accgccagcc ggatctgcgc cgccgccggc
     3301 aggtcgaagg cgatcgtcgt caccggattg aaggggttgg gatagttctg ctccagggcc
     3361 aggcgggccg gcagggtttc ggccggcatt tcaactgttg ttggtacaga ggtcctggtc
     3421 acgacgacga ggttgtcaac gaagatggcg ccgctgacgg ccgagtcagc ggtcttcatc
     3481 agctggatgc tgtcgatgct cagcttgctg ccgtcgagct ggttgttggt cgccagccag
     3541 gcgccgacca cgccgggatc gctcagtttc cattccacca gtcgccagcc gtaccagtcg
     3601 accttgaccc actgcgtcac ctccggcagg gtgtttttgg cgacc
//
LOCUS       DAOPWU010000337         3615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_26138_length_3615_cov_4.410674, whole genome shotgun sequence.
ACCESSION   DAOPWU010000337 DAOPWU010000000
VERSION     DAOPWU010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26138_length_3615_cov_4.410674"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1225
                     /locus_tag="PKM23_13285"
     CDS             311..1225
                     /locus_tag="PKM23_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNY92490.1"
                     /translation="MTRVEIKMARPLLVLLLLVILVMQGCLTTAKKVLPLNNRPPRIA
                     LVLGGGSSRGFAHVGVIRVLEQEKIPIDMIVGTSVGSLIGAIYAANPNSFELEWLAFE
                     LEKDDIFDFSILSSKTGPVKGNKLEKFVTEHVKIRHIEEMKIPYYAMAVDLNTGESVV
                     LEKGPVELAVRASASIPGIFTPLNINDRILVDGGVNGSIAPETAREKGADIVIAVNIG
                     KDITNYETGNIVQITLQAIDIMGKRIDEYKNRQADILIEPEVGNVGTMDFSKKKELMV
                     AGIEAGQKAIPAIKAAIEKWYTTPVAQR"
     gene            complement(1256..1879)
                     /locus_tag="PKM23_13290"
     CDS             complement(1256..1879)
                     /locus_tag="PKM23_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNY92491.1"
                     /translation="MQENYVITIGRQFGSGGHQIGRRLAERLGISFYDKELIRIASEQ
                     SGLKEEFFEQVDEKKRFSLFGGLLGSRGIASEEYNANFYLNNESLFRIQAEVIREIAG
                     KESALFVGRCADYILQNHPRAFHIFVCADLAERIRRVAERHSLTAGKAEELIAKTDKQ
                     RAAYYNYFTSKNWGAVASYHLCINSSFLGIEETVELIRQLTGKKFAL"
     gene            1971..2954
                     /locus_tag="PKM23_13295"
     CDS             1971..2954
                     /locus_tag="PKM23_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNY92492.1"
                     /translation="MADNNTITAVPGIRVGHATDAEGLTGCTVVLCDGEGAVGGVDQR
                     GGAPGTRETDLLRPMHLVQRVNAVLLAGGSAFGLAAADGVVRFLSEKGVGYKSGAVRV
                     PIVPAAILFDLGLGSAEARPGAEMGYTAALAASGHAVPEGNFGAGAGCSAGKLLGPGR
                     ATKTGIGSVAVRLGGGLVVGALMAVNPFGDVVAEDGNIIAGTRPLRGKGFAGSLKLLN
                     SLTGRLALRAAGATVIGVVATNARLNKEECNKMAQMAQDGLARAIRPAHTLFDGDTLF
                     ALATGKVRTSATLAGAYAAEMAAQAIVRAAECAVAAGGLPAGRDLLAGARA"
     gene            complement(3008..3478)
                     /locus_tag="PKM23_13300"
     CDS             complement(3008..3478)
                     /locus_tag="PKM23_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92493.1"
                     /translation="MEWIITMLLLAGMALFGYYYIRRFRRSEGGSEGKLMLVAVRRRE
                     VGMKRSLELSREEHEETTALLDRQWRIIHGRWIGLGAHYLQGFASDAAGIPAPGDQDW
                     RLFQLYGLNDYAMFRRCVAVLEEESNNLLRHHLEIRLIPGESMADVGGKVKRLI"
BASE COUNT          688 a         1077 c         1141 g          709 t
ORIGIN      
        1 gcgattattt cagttcggcc gaccagccga cgggtgcagg gagcaaagcg gaggaggccc
       61 gccgcaaatt ggatgccctc tttggcccca aaaagagctg agggcggaaa ggccactgct
      121 gtctgcagcc gtttggcaag gcgtgtgaag agccgctcgc cttggtgtgc cgctccggtt
      181 gcacagagcc gcttccgtca tgccgctttt ttgcttgaat ttggctcccc gattggctat
      241 tttacctcaa ttaatgagca gtgagggcgt caggtcagcc ctctggttaa aactaaggga
      301 gcagctcgag atgacaagag tggaaatcaa gatggcgcgg cccctgctgg tgctcctcct
      361 gctggtgatc ctggttatgc agggctgtct caccaccgcg aaaaaggtcc tgcccctgaa
      421 taaccggcca cccaggatcg ccctggttct tggtggaggc tccagccggg gattcgccca
      481 tgtcggggtc atccgcgtgc tcgagcagga aaaaatcccg atcgacatga tcgtcggcac
      541 ctcggtcggc agcctgatcg gcgccatcta cgccgccaat cccaacagct ttgagctgga
      601 gtggctcgcc tttgaactgg agaaggatga catcttcgac ttttccattc tctcttccaa
      661 gaccggaccg gtcaaaggca acaaactgga aaaatttgtc actgaacacg tcaagattcg
      721 ccacatcgag gagatgaaga tcccctacta cgccatggcc gtcgatctca acaccggcga
      781 gtcggtggtg ctcgaaaagg ggccggttga gctggcggtg cgcgcctcgg cctccatccc
      841 cggcatcttt actcccctga acatcaacga ccggatcctg gtggatgggg gggtcaacgg
      901 cagcattgcg cccgagaccg cgcgcgaaaa gggggccgac atcgtcattg cggtcaatat
      961 tggcaaggat attaccaatt acgagaccgg gaacatcgtc cagatcaccc tgcaggcgat
     1021 cgacatcatg ggcaagcgga tcgacgagta taaaaaccgg caggccgata ttctcatcga
     1081 gccggaagtg ggcaatgtcg ggacgatgga tttttccaaa aagaaggagc tgatggtcgc
     1141 gggcatcgag gcgggtcaga aggcgattcc ggcgatcaag gcggcgattg aaaagtggta
     1201 caccaccccg gttgcacaga ggtaatcccg gcgcggggcg gcttccctgc tttatttaca
     1261 gggcgaactt ttttcctgtg agctggcgga tcaactcgac tgtctcttca atgccgagaa
     1321 aagaggagtt gatgcagagg tggtaggagg ccaccgcccc ccagtttttg ctggtgaaat
     1381 agttgtaata ggcggcgcgc tgtttgtcgg ttttcgcaat gagctcttcc gccttgcccg
     1441 ccgtcaggct gtggcgttcg gcgacgcgcc tgatccgttc cgccagatcg gcgcagacga
     1501 agatatggaa ggcgcggggg tggttctgca gaatgtaatc ggcgcagcga ccgacgaaga
     1561 gggcggactc tttgcctgcg atctcgcgga tgacctcggc ctggatcctg aagaggcttt
     1621 cattgttgag gtagaaattg gcgttatact cctccgaggc aataccccgc gaacccaaca
     1681 gtcccccgaa gaggctgaac cgcttcttct catccacctg ttcgaagaac tcctccttga
     1741 gaccgctctg ctccgaagcg atgcggatga gctccttgtc gtaaaaggag atcccgagcc
     1801 gctcggccag tctgcgcccg atctgatggc cgccgctgcc aaactgccgc ccgatagtga
     1861 tgacatagtt ttcctgcatg aaaactctcc aggttgggag gctccgcttc cccgccggcg
     1921 ccctgacggc ttagtatagt aaagtctgtt ttttaacgca aggattattt atggctgaca
     1981 ataacaccat caccgctgta cccggcatcc gggtcgggca tgccaccgat gccgagggat
     2041 tgaccggttg cactgtggtg ctgtgcgatg gcgaaggggc ggtcggcggg gtggaccagc
     2101 ggggcggggc cccgggtaca cgcgagaccg acctgctgcg gccgatgcac ctggtgcagc
     2161 gggttaatgc agtgctgctg gccgggggca gcgctttcgg gctggccgca gctgacgggg
     2221 tggtgcgctt cctgagcgaa aagggggtgg gctataaaag cggggcggtg cgtgtgccga
     2281 ttgtgccggc cgccatcctc ttcgacctcg gtctgggcag cgctgaagcc cggcccggcg
     2341 cggagatggg ctacaccgct gctctggctg cttccggcca cgccgtcccg gagggaaatt
     2401 tcggcgcggg cgccggctgc agcgcaggca agctgctcgg ccccggtcgc gcgaccaaaa
     2461 ccggcatcgg cagcgtggca gtcaggttgg gaggcggcct ggtggttggg gcgctgatgg
     2521 ccgttaatcc cttcggagat gtcgtggcgg aggatggaaa catcatcgcg ggcacgcggc
     2581 ctctgcgcgg caagggcttc gccggttccc tgaaattgct gaacagcctt accggacgcc
     2641 tggccctgcg ggccgccggc gccacggtga tcggcgtggt ggcgaccaac gcccggctca
     2701 acaaggagga gtgcaacaag atggcgcaga tggcccagga cggcctggcc cgcgccatcc
     2761 gtcccgctca caccctcttc gacggggata ccctcttcgc cctcgccacc ggcaaggtgc
     2821 gcaccagcgc cactctggcc ggagcttatg ccgctgaaat ggcggcgcag gccattgtgc
     2881 gggcggccga atgcgccgtc gccgccggcg gactgcctgc cggcagggat ctcctcgccg
     2941 gcgccagggc ctgacgcggc tccggctgga tcgctctccc ctcggtacgg actcacggcg
     3001 ccctgcgtca gatcagccgc ttcaccttgc cgccgacatc ggccatcgac tcgccgggga
     3061 tgaggcggat ctcaagatgg tggcgcagca ggttgttgct ctcctcctcg aggaccgcga
     3121 cgcagcggcg gaacatggcg taatcattaa gaccgtagag ctgaaagagc cgccaatcct
     3181 ggtcgccggg ggcaggtatg ccggctgcat cgctcgcgaa accctgcaga tagtgggcgc
     3241 cgagacctat ccagcgtccg tggatgatgc gccactgcct gtcgagcagg gcggtggttt
     3301 cctcgtgttc ctctctgctc agctcgagac ttcgcttcat gcccacttcc cgccggcgca
     3361 cggcgaccag cataagcttg ccctcgctgc ccccctcact tctcctgaat ctccggatat
     3421 agtaatagcc gaaaagggcc atcccggcca gcagcagcat ggttatgatc cattccatca
     3481 caggctcctc aactttgggc gctttgcatg gggtaaaata gccgattaga ttctcaagtg
     3541 caagcgaatt tttggtaact cttgtaaagt tatcggcttc cggagaaccg agctgccacc
     3601 gggagggagt attta
//
LOCUS       DAOPWU010000338         3598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_26317_length_3598_cov_3.686988, whole genome shotgun sequence.
ACCESSION   DAOPWU010000338 DAOPWU010000000
VERSION     DAOPWU010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26317_length_3598_cov_3.686988"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1370
                     /gene="murF"
                     /locus_tag="PKM23_13305"
     CDS             42..1370
                     /gene="murF"
                     /locus_tag="PKM23_13305"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNY92494.1"
                     /translation="MQRPLSGVSTDSRTAQPGDLFVALAGERFDGHNFVLPRAAAGVS
                     VSMVSAAWFAQHAGETAAANLIVVPETLRSFQDAAAAYRATFSIPAVAVTGSVGKTTT
                     KEAIYAVLSRRFRVLRNAKSFNNHVGVPLTLFALRDEHQILLTELGTNHFGELDRLGA
                     LVKPEMAMITNIGHAHLEFFHDLAGVARAKFEIFNHCRPEGVAFYNADDPLLRGRHYP
                     LVRTLSFGIDHPADLTAEVVGCDAHARYTLRIQGQEVTLPVSGRHNVHNALAAAAVGM
                     EFGLGLDEILKGLAALPEVDKRMALSSSGGVLLMNDTYNANPNSCAAALRTLADISVQ
                     GSSRRIAVLGDMLELGGYSIQEHRKLADLAAGFGLDQLFLFGRETQATLERAEELRLV
                     VHHFSDKDELVQALTAILQPGDVILFKGSRGMLMETLVEGVHHHLATNFK"
     gene            1382..2467
                     /gene="mraY"
                     /locus_tag="PKM23_13310"
     CDS             1382..2467
                     /gene="mraY"
                     /locus_tag="PKM23_13310"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNY92495.1"
                     /translation="MLYYLLAPLKDSHIFFNLFRYISFRAAFAALLALALSLIIGPAL
                     IRKLREKQIGEEIRSDGPQTHLGKRGTPSMGGLLILVSTLVPTLLLAKIAETGVWVVM
                     IVTLWLGMLGLLDDYLKIVKKMPKGLIGRYKLVGQIVIGLFVGLVVYYLPAVDAERSS
                     TTIPFLKDFELEFGIFYIFMVAFVITAMSNGSNLTDGLDGLLIGLSAIGALAFGLIAY
                     VTGRVDFSDYLNIIYLPEAGELAIFCAALFGASLGFLWYNAYPAQVFMGDTGALALGG
                     AIGAVAVLVKKELLLLLICAIFVIESLSVIIQVTHFKRTGKRIFRMAPIHHHFELSGW
                     AEPKVVVRFWIVGIILALLTLTTFKIR"
     gene            2464..>3598
                     /locus_tag="PKM23_13315"
     CDS             2464..>3598
                     /locus_tag="PKM23_13315"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNY92496.1"
                     /translation="MIPAEKKMDWARADFALLACILLLIILGAATIYSASSYRMEMKF
                     GNSEYYFTKQLVRAFIGLLLMFFIAKRDYRKWLADRWILFGVSLFLLAVLLVRIPGIT
                     PRNGAYSWINLYFFMFQPSDFARYALILLLAGQLHLWRDELDDINRYAILFGLTLAIA
                     GPIALQHDMGTAALTLLIAFTMFILAEVRISYSIIAGLSTLSLALLYISVNPYMIKRL
                     TDHLHSTGGEMHPHLKQSLIALAQGGLIGQGIGGSHQKYLFLPEAHNDFIFAMIGEEY
                     GLIGTVGVLLLFLVVVHRGLTIARQAPDLAGRYLAGGITACYASYAFINAGVVVGLLP
                     TTGIPMPFLSYGGTSLVTHLCALGLLLNISAQGSPAFAASPGWR"
BASE COUNT          612 a         1168 c         1009 g          809 t
ORIGIN      
        1 gtgatcaggg cttgcagcgt ggccgttctg cctaggcgct gctgcagcgc ccgctctccg
       61 gagtctccac ggactcgcgc actgcgcagc ccggcgacct ctttgtcgcc ctcgccggtg
      121 aacggttcga cggccacaat ttcgtcctgc cccgcgctgc tgcgggtgtc tcggtctcga
      181 tggtctcggc cgcctggttt gcgcaacacg ccggagagac cgctgcagcc aacctcatcg
      241 ttgtgccgga gaccctgcgt tcctttcagg atgcggccgc cgcctatcgc gccacctttt
      301 cgatccccgc ggtcgcggtc accggcagcg tcggcaagac cacgaccaag gaggcgattt
      361 acgctgtgct ctcgcgccgg ttccgggtcc tgcgcaacgc caaatccttc aacaatcacg
      421 tcggcgttcc gctcaccctc ttcgcgctgc gtgatgagca tcagatcctg ctcacggagc
      481 tgggcaccaa ccacttcggt gagcttgacc gcctcggggc cctggtcaaa ccggagatgg
      541 ccatgatcac caatatcggc cacgcccatc ttgaattctt tcatgatctc gccggagtgg
      601 cccgggccaa gttcgagatt ttcaaccact gccgtcccga gggcgtggcc ttttacaatg
      661 ccgatgaccc gcttttgcgc gggcgccact acccactggt ccgcaccctc tcctttggca
      721 tcgaccatcc ggctgatctc accgccgagg tcgtcggctg cgatgcccat gcccgctata
      781 ccctgcgtat ccaggggcaa gaggtgactc tgccggtcag cgggcgccac aatgtccaca
      841 acgcccttgc agcggcggca gtcggaatgg agttcggact cggcctggat gagatcctca
      901 aagggctggc cgctctgccg gaggtggaca agcgcatggc cctgagctcc agcggcgggg
      961 tgctgctgat gaacgatacc tataatgcca atcccaacag ctgcgcagcg gccctcagaa
     1021 ctttggcgga tatctccgtg cagggcagta gccggcgcat cgctgtgctg ggggacatgc
     1081 tcgaactcgg cggttactcc atccaggaac accgcaaact ggccgatctc gccgccggct
     1141 tcggcctgga tcaacttttt ctcttcggcc gcgaaaccca ggccacgctc gaacgggctg
     1201 aagaactgcg gctggttgtc caccatttca gcgacaagga tgaactggtg caggccttga
     1261 ctgcgatcct tcagcccggg gatgtcattc tcttcaaggg atcgcgcggc atgctgatgg
     1321 aaaccctggt ggagggggtc caccaccatt tggcgacgaa ttttaaatag cggaactgac
     1381 catgctctac tatttgctcg cgcccctgaa agacagtcat atcttcttca accttttccg
     1441 ctacatcagt ttccgggcgg cctttgccgc gctgctggcc ctggccctga gcctgatcat
     1501 cgggcccgcc ctgatccgga aactgcgcga gaagcagatc ggcgaggaga tccgcagtga
     1561 cgggccgcag acccatctcg gcaagcgcgg caccccgagc atgggagggc tgctcattct
     1621 ggtgagcacc ctggttccga ccctgctgct cgccaagatc gccgagaccg gggtctgggt
     1681 ggtgatgatc gtgaccctct ggctgggcat gctgggtctg ctcgacgact atcttaaaat
     1741 cgtcaaaaag atgcccaaag gactgatcgg gcgctacaaa ctggtcggcc agattgtcat
     1801 cggcctcttc gtcgggttgg tggtttatta cctgcccgcg gtcgatgccg agcgctcgag
     1861 caccaccatc ccctttctca aggatttcga gcttgaattt ggcatctttt acatcttcat
     1921 ggtcgccttt gtgatcacgg cgatgtccaa cggctccaac ctgaccgatg gcctggatgg
     1981 cctgctcatc ggcctgagcg ccatcggagc cctcgcattc ggcctcatcg cctacgtcac
     2041 cggccgtgtc gatttcagcg actatctcaa catcatctat ctgccggagg cgggcgaact
     2101 ggcgattttt tgtgcggctc tcttcggcgc ctccctgggt ttcctctggt acaatgccta
     2161 tccggcccag gtcttcatgg gcgacaccgg cgctctggcc ctcggtgggg ctatcggcgc
     2221 tgtggcggtg ctggtcaaaa aggagctcct cctcctgctg atctgcgcca tcttcgtcat
     2281 cgagagcctg tcggtgatca tccaggtcac ccatttcaaa cgcaccggca agcgcatttt
     2341 ccggatggcg cccattcacc accacttcga actcagcgga tgggccgaac ccaaggtggt
     2401 cgtgcggttc tggattgtgg gtattattct ggcgcttcta accctgacga catttaaaat
     2461 aagatgattc ctgcagaaaa gaaaatggac tgggcgcgcg cggactttgc cctgctcgct
     2521 tgcatactcc tgctcatcat cctcggagcg gcgacgatct attccgccag ctcctatcgc
     2581 atggagatga agttcggcaa cagcgagtac tatttcacca aacagctggt tcgcgccttc
     2641 attggtctgc tgctgatgtt tttcatcgcc aaacgcgact accgcaagtg gctggctgac
     2701 cggtggattt tattcggtgt ctccctgttt ctgctcgccg tcctcctggt gcgcatcccc
     2761 ggcatcaccc cgcgcaacgg ggcctacagc tggatcaatc tctatttctt catgttccag
     2821 ccttcggact tcgcccgtta tgccctcatc ctgcttctgg ccgggcagct ccatctctgg
     2881 cgggacgaac tggatgacat caaccgctat gccatccttt tcggactgac cctggcgatc
     2941 gccggcccga tcgccttgca gcacgatatg ggcaccgcgg cgctgaccct gctcatcgcc
     3001 tttacaatgt tcattcttgc ggaggtcagg ataagctatt ccattattgc cggcttgagc
     3061 accctcagcc tggctttgct ttatatcagt gtcaatccct acatgatcaa acggctcacc
     3121 gaccacctcc acagcaccgg cggcgagatg catccccatc tcaagcagtc gctcatcgcc
     3181 ctggcgcagg ggggactgat cggacagggt atcggcggga gccatcaaaa gtatctcttc
     3241 ctgcccgagg cgcacaacga tttcattttt gccatgatcg gggaggagta cggactgatc
     3301 ggcacggtgg gcgtgctgct gctctttctg gtggtcgtcc accgtggatt gaccatcgcc
     3361 cggcaggcac cggatctggc cggtcgctat ctggccggtg ggatcaccgc ctgctatgcc
     3421 agttatgcct tcatcaatgc cggcgtggtg gtcggccttc tgcccaccac cggcattccg
     3481 atgccttttc tcagctatgg cggcacctcg ctggtgactc atctctgcgc cctcgggctc
     3541 ctgctcaata tctctgcgca gggcagtccc gcttttgccg ccagtccggg gtggcgcg
//
LOCUS       DAOPWU010000339         3586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_26435_length_3586_cov_4.199943, whole genome shotgun sequence.
ACCESSION   DAOPWU010000339 DAOPWU010000000
VERSION     DAOPWU010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26435_length_3586_cov_4.199943"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PKM23_13320"
     CDS             complement(<1..907)
                     /locus_tag="PKM23_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNY92497.1"
                     /translation="MAKMKALVFKRVGCIELEEIPIPEVREHDDVIVKVAACGICGSD
                     VKIMEGKHAYREGVVLGHEFCGTVVEVGKSVTAVKPGDRVAVDNNPRCGVCDFCRMGL
                     SSQCEIIKEKTLGIFQNGGYAEYCRAPESVCFKLPDVLDNMVATQVETLGTVLNGMNT
                     VQMQPWDAVLILGFGPIGYLFTGMAKNIAAQVAVSEIDPFRRGVARQLGVPVYDPDQT
                     DLVQMAKERTRGNGFDIVIDAVGTQLENALKYVAPGGKVLAFGMDSSVKATITPYEIT
                     RRAVRILGTYIGQNTMLPAIKILQEK"
     gene            complement(1102..2013)
                     /locus_tag="PKM23_13325"
     CDS             complement(1102..2013)
                     /locus_tag="PKM23_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNY92498.1"
                     /translation="MKKAILSLALLGLCIGCSQDKSAPAVALVMKTLNNPFFMDMQLG
                     AEEAAQRLQLPLIVQAAERELDVEKQMQIIENLIQRKVAAICLTPAGSREVIPAILKA
                     NRAGIPVLILDTRVDSTALAEAGGKVATFIGSDNFDGGRIAGERIIEKLGGIGRVAIL
                     EGIPGHETGDSRLRGFHAAIDKAPGIRIVASQTANWERDQGYNVFQNILQSHPDVQAV
                     FGCNDMMALGAVEAIAAAGRSADILVIGFDAITDAREAIAAGRMEASIAQNPREMGRL
                     AIENAARFMRGEQIPAYIPVPIELVRK"
     gene            complement(2029..2976)
                     /locus_tag="PKM23_13330"
     CDS             complement(2029..2976)
                     /locus_tag="PKM23_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HNY92499.1"
                     /translation="MRVKSLFLQHGRQLTTLFVLILLSAALWALSPHFMSLSNWLNIA
                     QQTAINAIIAAGMTFVIISAGIDLSVGSILALSGVVMASALQAGAPVPLALLLCLVVG
                     AQCGSANGLLIARGKLPPFIATLGMMSVARGAALIFSDGRPVSGFAPGFRAIANGEFL
                     AIPLPVWIMAAVYLIAHLVLSRTRLGRYTYAIGGNEEATLLSGVNVKLHKTLVYSLAG
                     LLSALAAILLTARLNSAQPIAGMNYELDAIAATVIGGTSLLGGEGQIFGTLIGAFIMG
                     VLRNGLNLLGVSSFVQQTVIGAVIIVAVLLDMMMKKRRS"
     gene            complement(2977..>3586)
                     /locus_tag="PKM23_13335"
     CDS             complement(2977..>3586)
                     /locus_tag="PKM23_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY92500.1"
                     /translation="ELARALFGADPVDGGRIFIKGKPVRIGSPRDAIHQGIGYLTEDR
                     KGQGLVLNLAVRENICLASLARFSRLGWVSRTRESNAASGFVDKLRIRTPGLGQQVRY
                     LSGGNQQKVVMSKWLCSESRILLFDEPTRGIDVGAKVEIYQLMNKLAADGAAILMISS
                     ELAEIIGMSDRVLVMCQGRIAGELSAAEATQQKILNLALGGN"
BASE COUNT          680 a         1103 c         1127 g          676 t
ORIGIN      
        1 ttttctcctg caggatcttg atcgccggca gcatggtatt ctggccgata taggtgccga
       61 ggatcctgac cgcccggcgg gtgatttcgt aaggggtgat ggtcgccttg acgctcgagt
      121 ccatgccgaa ggcgagaacc ttgccgccgg gggcgacata tttcagggcg ttctcgagct
      181 gggtgccgac ggcatcgatg acgatatcga agccgttgcc gcgagtccgc tctttggcca
      241 tctgcaccag atcagtctgg tcagggtcat agaccggcac accgagctgc cgggcgacgc
      301 cgcggcggaa ggggtcgatc tcgctgacgg ccacctgggc ggcgatgttt ttcgccatac
      361 cggtgaaaag atagccgatc ggtccgaaac cgaggatgag caccgcatcc cagggctgca
      421 tctgcacggt gttcatgccg ttgagcacag tgccgagggt ctcgacctgg gtggcaacca
      481 tattatccag aacatcagga agcttgaaac agacactctc cggagcgcgg cagtactcgg
      541 catagccccc gttctgaaaa atacccaggg tcttctcctt gatgatttcg cactggctgc
      601 tcagccccat gcggcagaaa tcgcacaccc cgcagcgggg gttgttgtcg acagcgacgc
      661 ggtcgccggg cttgactgca gtcaccgact tgcccacctc caccaccgtg ccgcagaatt
      721 catggccgag gacgaccccc tccctgtagg cgtgcttgcc ttccatgatc ttgacatccg
      781 agccgcagat gccgcaggcc gccaccttga caatgacatc gtcgtgctcc cggacctccg
      841 ggatggggat ctcctcaagt tcgatgcagc ccaccctctt gaaaaccagc gctttcatct
      901 ttgccatctt tttctcgctt tccttgaaga taatatgagt attcgcgcca cgagcgtaaa
      961 acatcactct caccaagaca ccaagccaca aagaaatata tatttagatc ttctttgcgc
     1021 ctttgcggct ttgcgagaga catttcctgt cttttctttg cgtctctgcg tctcagcggg
     1081 aggccgctgc tatgacttat tctactttct caccagctcg atcgggaccg ggatgtaggc
     1141 cggaatttgc tcgccccgca tgaagcgggc ggcgttttca atcgccagcc ggcccatctc
     1201 gcgcggattc tgggcgatcg aggcctccat gcggccggcg gcgatggcct cacgggcatc
     1261 cgtgatggcg tcaaagccga tcacaaggat atccgcgctg cgccccgccg cagcgatcgc
     1321 ctctaccgcc cccagggcca tcatgtcgtt gcagccaaag accgcctgga catcgggatg
     1381 ggactggaga atattctgaa agacattgta gccctgatcc cgttcccagt tggcggtctg
     1441 ggaggcgacg atgcggatcc ccggcgcctt gtcgatcgcg gcatggaagc cgcgcaggcg
     1501 agaatcgccg gtctcatggc cggggatgcc ctcgaggatg gctacccggc cgattccgcc
     1561 cagtttttcg atgatgcgct cgccggcgat gcggccgccg tcaaaattgt cggagccgat
     1621 gaaggtggcc acttttccac cggcctcggc cagggccgtc gaatccacgc gcgtatccag
     1681 gatcagcacc gggatgccgg cccggttggc cttgagaatg gccggaatca cctcgcgcga
     1741 accggcgggg gtgaggcaga tcgccgcgac cttgcgctgg atgagattct cgatgatctg
     1801 catctgcttt tcgacatcca gctcgcgttc agccgcctgg acgatgagcg gcagctgcag
     1861 gcgttgagcc gcctcttcgg ccccaagctg catatccatg aagaaggggt tgttgagggt
     1921 cttcatgacc agggccaccg ccggggcgga cttgtcctgg ctgcagccga tgcaaagccc
     1981 gaggagggcg agtgacagga ttgctttttt catggtcttc tcctgatttc aggatcgtct
     2041 ttttttcatc atcatgtcga ggagaacagc gacgatgatc accgccccga tcaccgtctg
     2101 ctggacaaac gaggagacgc cgagaagatt gagtccgttg cgcaaaaccc ccatgataaa
     2161 ggccccgatc agggtgccga agatctgccc ctcacccccg agcaggctgg tgccgccgat
     2221 cacagtggcg gcgatggcgt ccagctcata attcatcccg gcgatgggct gcgccgaatt
     2281 gaggcgggcg gtgagcagga tggccgccag ggcgctgagc agcccggcga ggctgtagac
     2341 cagggttttg tgcagtttga cgttcacgcc cgaaagcagg gtcgcctcct cgttgccgcc
     2401 gatggcatag gtgtagcgtc ccaaccgggt ccggctgagc accagatggg cgatgagata
     2461 gaccgccgcc atgatccaga ccgggagggg gatggcgaga aactccccgt tggcgatcgc
     2521 cctgaagccg ggcgcaaagc ccgagaccgg ccggccgtca ctgaagatga gggcggcgcc
     2581 gcgcgcgacg ctcatcatgc cgagggtggc gatgaaaggt ggcaatttgc cgcgggcgat
     2641 gagaagaccg ttggcgctcc cgcactgggc gccaaccacc aggcagagga ggagggccag
     2701 cgggaccggc gcccccgctt gcagagcact ggccatgacc acccccgaga gggcaaggat
     2761 ggagcccacc gagaggtcga tgccggcgct gatgatgaca aaagtcatac ccgcagcgat
     2821 gatcgcattg atggccgtct gctgggcgat gttgagccag ttcgagagcg acatgaaatg
     2881 gggggagagc gcccagaggg ctgccgagag caggatgagg acgaacaggg tggtgagttg
     2941 gcgtccgtgc tgcaggaaaa ggctttttac acgcactcag ttgcctccca aagcgaggtt
     3001 caaaatcttt tgctgggtag cctcggcggc ggagagctcc ccggcgatgc gcccctggca
     3061 catcaccagc acccggtcgc tcatgccgat gatttccgcc agctcggagg agatcatcag
     3121 aatggcggcg ccgtcggccg caagcttgtt catgagctgg tagatctcca ccttggcccc
     3181 gacatcgatg ccgcgggtgg gctcgtcgaa aaggaggatt cgcgactcgg agcagagcca
     3241 cttgctcatc accactttct gctggttgcc cccgctcagg taacggacct gctgccccag
     3301 gccgggggtg cggatgcgca gcttgtcgac gaaaccggag gccgcgttgc tctcccgcgt
     3361 ccggctcacc caacccaggc gcgagaacct ggccagactg gccaggcaga tattctcccg
     3421 gaccgcgaga ttgaggacca gtccctggcc cttgcggtcc tcggtgagat aaccgatccc
     3481 ctggtggatg gcgtcacggg gcgagccgat gcggaccggc ttgcccttga tgaagatgcg
     3541 gccgccgtcg acgggatcag ccccgaacag ggcccgcgcc agttcc
//
LOCUS       DAOPWU010000340         3569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_26606_length_3569_cov_3.541833, whole genome shotgun sequence.
ACCESSION   DAOPWU010000340 DAOPWU010000000
VERSION     DAOPWU010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26606_length_3569_cov_3.541833"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..545)
                     /locus_tag="PKM23_13340"
     CDS             complement(141..545)
                     /locus_tag="PKM23_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92501.1"
                     /translation="MQADSAWETFARTVHAGKKIAVTLVDSAGVTGRLLAVDPQSLRI
                     EPAGTTKVIPAARIVRIRYAGLRTRNTLYGMLAGLAVGAIATVVIDQNSAHPSSVGEA
                     AGMGGFLLGLPIGAIAGALIPVGPPLYERPGE"
     gene            complement(765..2099)
                     /locus_tag="PKM23_13345"
     CDS             complement(765..2099)
                     /locus_tag="PKM23_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNY92502.1"
                     /translation="MKWMIAMALMIAFSGEAAEQVVGYYPSWIRYNTPATKIRYQDLT
                     TIAHAFIWPKADGSLDMYSDLLYPEMNTRAHAAGVKVIVSIGGWGQCDGYPPMAASAA
                     TRKTFIDNVIAFMQKHGYDGIDLDWEYPSGTAQRKNFTLLAQEFRAAFDAVNPAWTIS
                     FVVPSGSYSGNNFDYTALRGLISWIGCMTYDIHGAWTAHAGHNSPLHAPANEPEGSID
                     TAVGYLLGMGIPREKLLIGVPFYGRQFAASRLYGSASGGEEITYAKLMTAVKPDWTRQ
                     WDDLAKVPYYQDGARTLFITYDDTASVRYKCDYVRDNKLGGLIIWALGQDNLGNTQPL
                     CQTVGDHLTRNTAVVATAEEARPEGYDLLSCYPNPCNQMVTVRYYLAKAGEAVLHLYD
                     VTGREIRLVEVGRREAGWYELPLSGEGLASGVYLLRMTATGHSATGKFTLVR"
     gene            complement(2154..>3569)
                     /locus_tag="PKM23_13350"
     CDS             complement(2154..>3569)
                     /locus_tag="PKM23_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNY92503.1"
                     /translation="NPVAKVPHRDRLMRLEAPVNGTPVEVYKAEHRITGWQNGWGAAG
                     GTLMLTQQERMRRWLYTWLLDVDKGTARLWFDLNEDDRYGSPGSPLLRPLPDGGWVLR
                     QKGSGIYFRGSGATQQGDRPFLDLRDMKTGVVERLFRCDPERYDYFVAFSGDESRFIM
                     SSESAVDVPNYYLASLGKKITAPKGEAARILSRVTITRFEDPAPQLRRIEKRIVRYER
                     KDGVPLSFQLYLPPGYQEGTRLPTVLYAYPLEFSDPATAGQVSGSTRRFNRISGASHL
                     FFLLAGYAVLDNTAMPMIGDPETTYDTFVPQLVADAEAAVAKAVEIGVADPERIGVIG
                     HSHGALMVANLLAHTDLFSAGIARSGSFNKTNQPFGFQSERRSLFEARDAYIQLSPTF
                     FADKIKEPILVIHGDADANPGTLTAQSEVFFEAVRGSGGTTRLVLLPYEDHGYRARES
                     IEHVLWEQLEWFDRYVKNEGR"
BASE COUNT          665 a         1037 c         1185 g          682 t
ORIGIN      
        1 aggggaatta cgactcttga tttaatagta atctagccaa gttttcatcg aaataacaaa
       61 aggttaaatc cgggaaaccg tcaaaaacct cgtaaatcag cagaatcatt tatcaacggc
      121 aaagttcagt tcgctattgg ttactccccc ggcctttcat aaagcggcgg gcccaccgga
      181 atcagagcgc cggcgatggc cccgatgggc agtccaagca ggaatccgcc cattcccgcc
      241 gcctcgccga cgctggaggg atgggcggag ttctggtcga tgaccacggt ggcgatcgcc
      301 ccgaccgcca gtcccgccag catgccataa agcgtgttgc gtgtgcgcag gccggcatag
      361 cggattctga cgatgcgggc ggctgggatg actttcgttg tgccggccgg ttctatacgg
      421 agcgattgcg gatcgacggc gagcagcctg ccggtcacgc cggccgaatc caccagagtg
      481 acagcaatct ttttacccgc gtggaccgtg cgggcgaagg tttcccaggc gctatcagcc
      541 tgcatcagcg ctgaagctcc cgccaccggc ggctgcgcct gcagctgcgc attcccaaac
      601 gtctgcgccg ttgattcgga agcggaggtt tgtgcggccg cgatggaggc gtgcgcaaga
      661 ataagtacgg ccgccatcgc tatgcgagag gcaagcttgt ccagggacat ggtagtcctc
      721 cataaaaatc gaaagttgct ccagattgag gcggaggcgc ggccctagcg cacgagggtg
      781 aacttgccgg ttgcgctgtg cccggttgcg gtcatccgca gcagatagac gccagaggcg
      841 agtccttccc ccgagagcgg gagttcgtac cagcccgcct cccgccggcc aacctcgacg
      901 aggcggatct cccgcccggt cacatcatag aggtgcagta cggcctcccc cgcctttgca
      961 agatagtagc gcacagtgac catctgattg cagggattgg gatagcagct gagcaggtcg
     1021 tatccttccg gccgggcctc ctccgctgtt gccaccactg cagtattacg ggtgagatgg
     1081 tcgccgacgg tctggcagag tggctgcgtg ttgccaagat tatcctgacc cagagcccag
     1141 atgatcagtc cgcccagttt gttgtcgcgc acataatcgc atttatatcg caccgaggcg
     1201 gtgtcgtcgt aggtgataaa gagggtgcgc gccccatcct gataataagg cacttttgcg
     1261 agatcgtccc actggcgggt ccagtccggt ttaacggcag tcatgagttt ggcgtaggtg
     1321 atctcctcac cgccgctggc ggaaccgtag aggcggctgg cggcgaactg gcggccgtaa
     1381 aaggggacgc cgatgagcag cttttccctg gggataccca ttccgagcag gtagccgacc
     1441 gcggtatcga tcgaaccctc gggttcattg gccggggcat ggagggggga gttgtggccg
     1501 gcatgggcgg tccaggcccc gtggatgtcg taggtcatac agccgatcca gctaatcagg
     1561 ccgcgcaggg cggtataatc aaagttgttt ccgctatagc tgccgctcgg cacgacaaaa
     1621 ctgatcgtcc aggcagggtt gaccgcgtcg aaggccgcgc ggaactcttg cgccagcagg
     1681 gtgaaattct tgcgctgcgc cgtgccgctg gggtactccc agtccagatc gatgccgtca
     1741 tagccgtgct tttgcatgaa ggcgatcaca ttatcgatga aggttttacg cgtggccgca
     1801 gatgccgcca tcgggggata gccgtcgcac tgcccccagc caccgatgga gacgatcacc
     1861 ttgaccccgg ccgcatgagc ccgggtgttc atttcgggat agaggaggtc gctgtacata
     1921 tcgaggctgc cgtccgcttt aggccagatg aaggcgtggg cgatggtggt gaggtcctga
     1981 taacgaattt ttgtcgccgg agtgttgtaa cggatccacg aagggtagta gccgaccacc
     2041 tgctcggctg cctctccgct gaaggcgatc atcagggcca tggcaatcat ccatttcatc
     2101 aggttttcct tatcctcttt ttatcggtca tcagtacgag cgccagcctt ccgctatctc
     2161 ccctcattct ttacatagcg gtcgaaccat tccagctgct cccagaggac atgctcgatg
     2221 ctctcgcggg cccggtagcc atggtcctca taggggagca gcaccagccg ggtcgtgccg
     2281 ccggatccgc gcacagcctc gaaaaagacc tcggactgtg ccgtcagggt gcccggattg
     2341 gcgtcggcat cgccgtggat gacgaggatg ggctccttga tcttatctgc gaaaaaggtg
     2401 ggcgagagct ggatgtaggc gtcgcgggcc tcaaagaggg agcggcgctc ggactggaag
     2461 ccgaagggct ggttggtctt gttgaaggag ccgctgcggg cgatgccggc gctgaagaga
     2521 tcggtgtggg ccaggaggtt ggccaccatc agggcgccat ggctgtggcc gatgaccccg
     2581 atacgctcgg gatcggccac gccgatctcc accgccttgg ccactgcggc ctcggcatcc
     2641 gccaccagct gcggaacaaa ggtgtcgtag gtggtctcgg gatctccgat catgggcatg
     2701 gcggtgttgt cgagcaccgc atagccggcc aggaggaaga agaggtggga ggcgccgctg
     2761 atgcggttga agcggcgggt cgagccgctc acctgtcccg cggtggcggg atcggagaac
     2821 tcgaggggat aggcgtaaag gacggtgggc agccgcgtcc cctcctgata accggggggc
     2881 agatagagct ggaagctgag gggcacgccg tccttgcgct catagcggac gatccgcttt
     2941 tcgatccggc gcagctgcgg cgccggatcc tcaaatcggg tgatggtaac ccggctgagg
     3001 atgcgtgccg cctcgccttt gggcgccgtg atttttttgc ccagggaggc caggtagtag
     3061 ttgggcacat cgacagccga ttcggagctc atgatgaagc gggactcatc cccggaaaag
     3121 gcgacgaaat agtcatagcg ttcggggtcg cagcggaaga ggcgctcgac cacacccgtc
     3181 ttcatatcgc gcaggtcgag gaagggccgg tcgccctgct gggtggcgcc gctgcccctg
     3241 aaatagatcc cgctgccctt ctggcgcagc acccagccgc catcgggcag ggggcggagc
     3301 aggggggagc ccggcgaacc gtagcggtca tcctcgttga ggtcgaacca gaggcgcgcc
     3361 gtgcctttat cgacgtcgag cagccaggtg tagagccagc ggcgcatgcg ctcctgttgg
     3421 gtgagcatga gggtgccgcc ggcggcgccc cagccgttct gccagccggt gatgcggtgc
     3481 tcggccttgt agacctcgac cggggttccg ttaacggggg cctccaggcg catgagccgg
     3541 tcgcggtgag ggaccttggc gacggggtt
//
LOCUS       DAOPWU010000341         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_26677_length_3562_cov_4.431993, whole genome shotgun sequence.
ACCESSION   DAOPWU010000341 DAOPWU010000000
VERSION     DAOPWU010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26677_length_3562_cov_4.431993"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /gene="serC"
                     /locus_tag="PKM23_13355"
     CDS             <1..802
                     /gene="serC"
                     /locus_tag="PKM23_13355"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNY92504.1"
                     /translation="DYVVTGEWAKRALKEANIVAKGRVAASSEERNFSYLPRAESWDL
                     DPAAAFVHVTSNNTIFGTQYRTFPALKDKFLICDMSSDILSHRIDVSQFAMIYAGAQK
                     NLGPAGVTLVILRNDLAATAREGLPTMLSYATHIKNNSLYNTPPCFPIYIMQLTLQWV
                     KEIGGLAAIEKINRDKAELLYSTIDESGGYYRGTVEPESRSWMNVTIRMASEELEAAF
                     IAEAKAQGLGGLKGHRSVGGIRVSMYNAMPLAGIEKLTAFMKAFKASH"
     gene            complement(870..1709)
                     /locus_tag="PKM23_13360"
     CDS             complement(870..1709)
                     /locus_tag="PKM23_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92505.1"
                     /translation="MKKIVLAVVLCSAVLAFAQNGTTLRYNYETGKAYNYAVTSNTVV
                     TQDIGGREVVTEMGQEAQFTIQPQSRAENGDYTCWISFPALKAKIKNFQMDTTLVMTA
                     LLNKRAEVVQTETGKVLSSTMVDTVAGMDMLMQQLGVEPGVLFKRIQVKLPQDAVAVG
                     GSWSESEPDSLLQGGINIAITPNTTYTLLGTEERAGLPCLKIAFKGTIAMSGRGSQMG
                     ADIALEGEGNQEGVIYFSQPQGILVSAESSADQNSTISITGPATMTIPQVTTVKSSLT
                     LLP"
     gene            complement(1726..3312)
                     /locus_tag="PKM23_13365"
     CDS             complement(1726..3312)
                     /locus_tag="PKM23_13365"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNY92506.1"
                     /translation="MDKNGWLVFLLVLALGALSTGAAGDLPSRAVASIRAEEMRVQLD
                     FLAADKLLGRNTPGAGLDTAAAFIARTFQMCGLTPVDGSYFHEFSLHKIFLGDTNSCR
                     ITGIDGQLRDYLIKKEFMPFEMTADKNCSGEILFAGYGITDPESGYDDYAHIDARGKI
                     VFVLKRGPRQNDPASPFWIHKEVSLTRVDEKIRNAIAHGAAGILLATDPLHNRLLMPR
                     GFPWPNLYKGFPPDAVPVTVGGMEAEKIPAIQVGEEAMVQLFGSIDALRAEQAAIDSA
                     MAPHSRILAGCRADIRTSTRHSVQHACNVVGLLEGRDPRLKEEVVIIGAHYDHIGCSK
                     SGTAGQDSIYNGADDNASGTVGVLAAARALTTAGKRPGRSILFITFAGEEKGLFGSNQ
                     YVKEPLFPLPATAAMLNMDMIGRNHPDSLTITGHLSSHELEKALHKANSRVGFLLDLS
                     REGAMRGSSDHAPFARKQIPVLFFHSGLHADYHKVSDESDKCDITKMARVAELCSRVA
                     WLIADREEKPLFTPPAKPVK"
BASE COUNT          712 a         1055 c         1049 g          746 t
ORIGIN      
        1 cgactatgtc gtcaccggcg aatgggccaa acgggcgctc aaggaggcca atatcgtcgc
       61 caaaggccgc gtggccgcct cctctgagga gaggaacttt tcctacctgc ccagggcgga
      121 gagttgggat ctcgatcccg ctgccgcttt tgtccacgtc acctcgaaca acaccatctt
      181 cggcacccag taccgcacct tcccggcgct caaggacaaa tttctcatct gcgacatgtc
      241 ttcggacatt ctctcacacc gcatcgacgt ctcccaattt gcgatgatct atgcaggcgc
      301 gcagaaaaac cttggcccgg ccggcgtcac cctggtgatt ctccgcaatg accttgctgc
      361 tactgcgcgg gaaggactgc cgaccatgct ctcctatgcc acccacatca agaacaactc
      421 gctctacaat accccgccct gctttccgat ttacatcatg cagctcaccc tgcagtgggt
      481 gaaggaaatc ggcgggttgg ccgcgattga aaaaatcaac cgcgacaagg ccgagctgct
      541 ctacagcacc attgacgaga gcggcggata ttaccgcggc acggtcgaac ctgaatcgcg
      601 ctcctggatg aacgtgacca tccgcatggc cagcgaggaa ctggaagcgg ccttcatcgc
      661 cgaggccaag gcccagggcc tgggcgggct caagggtcac cgttcagtgg gcggcatccg
      721 cgtctcgatg tacaacgcca tgcccctggc cggcatcgaa aagctgaccg ctttcatgaa
      781 ggccttcaaa gccagccatt aggccgcgaa tgatctaaaa aaaaggcgct gagatgctca
      841 gcgccttttt ttgttgaccc aaagcccgct cagggcaata gagtgaggct cgatttgacg
      901 gtagtgacct gcggaatggt catggtggcg gggccggtga tggagatggt cgagttctgg
      961 tccgcgctgc tctcggcaga gacgagaatc ccctggggct gggagaaata gatcaccccc
     1021 tcctggttcc cctcaccctc aagggcgata tcggccccca tctggctgcc gcgaccgctc
     1081 atcgcgatag tgcccttgaa ggctatcttg aggcagggaa ggccggcgcg ctcctcggtg
     1141 ccgaggaggg tgtaggtggt gttgggggtg atggcgatat tgataccgcc ctgcaaaagg
     1201 gaatccggtt cactctcgct ccagctccca ccgaccgcga ccgcatcctg cggcagtttg
     1261 acctgaatgc gcttgaagag gacgccgggt tcgaccccga gctgctgcat gagcatatcc
     1321 atccctgcaa cagtgtcgac catggtggag gagagcacct tgcctgtctc tgtctggaca
     1381 acttccgcac gcttgttaag aagtgcggtc atgaccaggg tggtgtccat ctggaaattc
     1441 ttgattttgg ccttaagggc cggaaaggag atccagcagg tatagtcgcc attttccgcc
     1501 ctggattggg gctggatggt gaattgggcc tcctgtccca tctcggtgac gacctcgcgc
     1561 ccgccaatat cctgggtgac gacggtgttc gaggtgacgg catagttgta ggccttgcct
     1621 gtctcatagt tataacgcag ggtggtcccg ttttgagcga aagcgagaac agcgctgcag
     1681 agcacaacgg ccagcacaat ctttttcatc cattcctcct gttgcttatt ttaccggctt
     1741 ggccggcggg gtaaataacg gtttctcttc tcgatcagcg atcagccagg cgacgcggct
     1801 gcacagctcc gcgacccggg ccatcttggt gatatcgcac ttgtccgact catcactcac
     1861 cttgtggtag tcggcgtgca gtccggaatg gaaaaagagc accggaatct gtttgcgcgc
     1921 aaagggggcg tggtcgctgc tgccccgcat ggccccctcc ctgctcagat cgagcaggaa
     1981 tccgaccctg ctgtttgcct tgtgcagggc tttttcgagc tcatgactgg agagatgccc
     2041 ggtgatggtg agggaatcgg gatggttgcg gccgatcata tccatattga gcatggcggc
     2101 cgtggcaggg agcgggaaga gaggctcctt gacatattga ttcgatccga aaagcccctt
     2161 ctcctcgcct gcgaaggtta tgaaaaggat ggagcgtccg ggtctcttgc cggctgtagt
     2221 gagcgccctg gcggcggcca gaactccgac agtgcccgaa gcgttgtcgt cagcgccgtt
     2281 gtagatgctg tcctgaccgg cggtgccact cttgctgcaa ccgatatgat cataatgggc
     2341 cccgatgatg accacctcct ctttgaggcg ggggtccctc ccctcgagca ggccgaccac
     2401 attgcaggca tgctggacgg agtggcgcgt cgaggtccgg atatccgctc ggcagccagc
     2461 cagaatgcgg gaatgcgggg ccatcgcgct gtcgatcgcc gcctgttcgg cccggagggc
     2521 gtcaatgctg ccgaaaagct ggaccatcgc ctcctcgccg acctgaatgg cggggatctt
     2581 ttcagcctcc attccgccca ctgtcaccgg gaccgcgtcc ggagggaagc ccttgtagag
     2641 attaggccag ggaaagccgc gcggcatcag caggcggttg tggaggggat cggtcgccag
     2701 cagaatgccg gcggccccgt gtgcgatggc attcctgatc ttttcatcga cccgggtgag
     2761 gctgacctcc ttgtggatcc agaaagggct ggcgggatcg ttctggcgcg gccctcgctt
     2821 gaggacgaag acgatcttgc ccctggcatc gatgtgggca tagtcatcgt atccggattc
     2881 cggatcggtg atgccgtagc cggcaaagag gatctcaccg ctgcagtttt tgtcagcggt
     2941 catctcgaag ggcatgaact ccttcttgat gaggtaatcg cgcagctgac cgtcgatgcc
     3001 ggtgatgcgg cagctgttgg tatccccgag gaagatcttg tgcaggctga attcatgaaa
     3061 atagctgccg tccaccgggg tgagaccgca catctgaaaa gtgcgggcga tgaaagccgc
     3121 cgcggtgtcg aggccggctc cgggggtatt gcgcccgagc agcttgtcgg cagcgagaaa
     3181 atcgagctgt actcgcatct cctcagcccg gatagaggct actgcccttg agggcagatc
     3241 gcccgccgcg cctgtagata gagcccccag tgccagcacg agcaaaaaaa ccagccaacc
     3301 gtttttatcc atcccgtcga ttccgttcct aatactgcga gaactccgaa gtattgctca
     3361 ctaaagcgtt aatatacata tccctcttgg caattgcaag cctcttgctc ggaaagggtg
     3421 ccccccgaaa tctggctccc acctgccatc ttgtgccgcg caccccccgc aaactggttc
     3481 cccatcttcc actgagacca gcgaaattct tgtcattctc actcaatata gctaattaca
     3541 gtgagaatga caagaatttc gc
//
LOCUS       DAOPWU010000342         3553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_26779_length_3553_cov_4.352773, whole genome shotgun sequence.
ACCESSION   DAOPWU010000342 DAOPWU010000000
VERSION     DAOPWU010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26779_length_3553_cov_4.352773"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="PKM23_13370"
     CDS             complement(<1..1552)
                     /locus_tag="PKM23_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY92507.1"
                     /translation="MTLHEEEVIGKAYDSRLMRRLLAYLHPYRWMVVLAVLILLLGSF
                     AELLLPYLTKVAIDRYIANKELAGLARLALLYFTVLLVQMSFIFFQTYLTQKIGQKVM
                     YDMRNQIFAHLQRLPLSFFDKNPVGRLVTRATNDVETLNSMLSSGVVTIFGDIFVLVG
                     IIAMMLIFNWQLALLSFTVLPLIFAASFWFRVHVRESFRKVRLRIARINSYLQENIMG
                     MSTVQIFNREKKNFRRFDELNNDYLQAYLETIYYYSVFHPLVELISALALAIILWYGG
                     IRVISGALTIGVVVAFVQYAERFFQPIRDLSEKYNLMQAAMASSERIFKLLDEPEQRL
                     LPENPAEMPPPCGEIEFRDVSFGYNENDHVLRNVSFKINCGEKVAIVGATGAGKTTLI
                     NLISRLYEPTSGRILLNRIDTALYPLDNLRRRVRMVLQDVFIFSGTVEYNIRLGDQSI
                     PMERVIQAAKDVRAHEFIEKLPQGYQTELSERGSNLSVGQKQLLSFARALVFDPEVLI
                     MDEATSSVD"
     gene            complement(1561..3393)
                     /locus_tag="PKM23_13375"
     CDS             complement(1561..3393)
                     /locus_tag="PKM23_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY92508.1"
                     /translation="MAPTIMPPTGKPLRFILHYLEQHKGTLMLGLLLTLLTSGFQLAS
                     PMVLRYAIEYAEWRLGSAGAALPAWILALTEGHTPLLILGLFALAMVVISLFQGIFRY
                     HLRMQLIGMSRRVEYALRNDYLAHLQRLSAGFFQRHKTGDLMARATNDMEAVRSMVGP
                     GIMYFATTFVTAIISIALMATLSWRTTLWALLLMPLVAIIVGRQVGQIEKLFDRIQEQ
                     FSTLTAKVQENISGIRVVKSYVQEGHEIDDFRAISREYIRRNLKLVLVRSGMWSSIDF
                     LLGLTTLAALFAGGREVMAGRLSIGGLVAFLSYLAMLAWPMIAVGWTLNMWKEGLAST
                     ERILAIFREKPEVADGPETDYSITELQGEIEFRSLSFAYSEGAPQVLKEISLHIPAGT
                     TVAIVGATGSGKSTLVNLIPRLYQAGKEGLLLDGHPIETIPLDLLRRSIGMVPQETFL
                     FSETLQENIAFGEEEIRMAEVEEAAETSQIRSDFDQFPEGFTTMVGERGITLSGGQKQ
                     RTAISRAIIRRPKILILDDALSAVDTYTEEEVLKRLRRIMAERTTILVSHRISTVRDA
                     DLIVVLREGRIIEQGQHDELLARKGAYWQLYQRQMLEESLAEIN"
BASE COUNT          739 a          974 c         1170 g          670 t
ORIGIN      
        1 tgtcgaccga ggaggtggcc tcgtccatga tcagcacctc aggatcgaag accagggcgc
       61 gggcgaagga gaggagctgc ttctggccca ctgaaaggtt gctgccgcgc tcgctgagtt
      121 cggtctgata gccctgcggc agcttttcga tgaactcatg cgcgcgcaca tctttcgcgg
      181 cctgaatgac ccgctccatc gggatcgact ggtcgccgag gcggatgttg tactcgaccg
      241 tcccggaaaa gataaagaca tcctggagca ccatgcgcac gcgccggcgc agattgtcga
      301 gggggtagag ggccgtatcg atacggttga gcaggatccg gccggaggtg ggctcataga
      361 gccgcgagat caggttgatc agggtggttt tgcccgcacc ggtggcgccg acgatggcca
      421 ccttttcgcc gcagttgatt ttgaaactca cattgcgcag gacgtgatcg ttttcgttgt
      481 agccaaaaga gacatcgcgg aattcgatct cgccgcaggg gggaggcatc tcggccggat
      541 tctccggcag caggcgctgc tccggttcat ccagcagctt gaagatgcgc tcgcttgaag
      601 ccatggcggc ctgcatgaga ttgtatttct ccgagagatc gcggatgggc tggaaaaaac
      661 gctcggcata ctggacaaaa gcgaccacca cgccgatggt caacgccccg ctgatcaccc
      721 tgatgccccc gtaccagagg ataatggcaa gagcgagggc gctgatcaat tcgaccaggg
      781 ggtgaaagac cgaataatag tagatggtct cgagataggc ctgcaggtag tcgttgttga
      841 gctcatcgaa acggcgaaag ttcttcttct cgcggttgaa gatctgcacc gtcgacatcc
      901 ccatgatgtt ctcctggaga tagctgttga tgcgcgcaat gcgcagcctc accttgcgga
      961 aggattcgcg cacatggacg cgaaaccaga agctggcggc gaagatcagg ggcagcaccg
     1021 tgaacgagag cagcgccagc tgccagttga agatcagcat catggcgatg atgcccacca
     1081 gcacaaagat gtcgccgaag atggtgacaa ccccgctgga aagcatgcta ttaagggtct
     1141 cgacgtcgtt ggtggcgcgg gtcaccagcc gtccgaccgg attcttgtca aagaaggaga
     1201 gggggaggcg ctgcaggtgc gcgaagatct ggttgcgcat gtcgtacatc accttttggc
     1261 cgatcttttg ggtcagatag gtctggaaaa agatgaagct catttgcacc agcagcacgg
     1321 tgaaatagag gagagccaga cgggccaggc cggccagctc cttgttggcg atgtagcggt
     1381 cgatggctac cttggtcagg taggggagga gcagttccgc aaacgagccg aggagcaaga
     1441 tcaggacagc gagcaccacc atccagcggt agggatggag ataggcgagc agccgccgca
     1501 tcagacggct gtcgtaggcc ttgccgatca cctcttcttc atgcagtgtc acggagcacc
     1561 tcagttgatc tcggccagcg actcttccag catctggcgc tggtacagct gccagtaggc
     1621 gcccttgcgc gctaaaagtt cgtcatgctg gccctgttcg ataatcctcc cctcgcgcag
     1681 aaccacgatc agatcggcgt cccgtacggt cgagatgcgg tgcgagacca ggattgtggt
     1741 ccgctcggcc atgatgcgcc gcagccgttt cagtacctcc tcctcggtgt aggtatcgac
     1801 cgcggagagg gcatcgtcga ggatgagaat tttgggacgg cggatgatgg cgcgtgagat
     1861 ggccgtgcgc tgtttctgcc cgccggagag ggtgatgccg cgctccccga ccatggtggt
     1921 aaagccctca gggaactggt caaagtcgct gcggatctgt gaggtctcgg ccgcctcctc
     1981 gacctccgcc atgcgaatct cctcctcccc gaaggcgatg ttctcctgca gggtctccga
     2041 aaagagaaag gtctcctgcg gcaccatgcc gatactgcgg cggagcagat cgagggggat
     2101 ggtctcgatg ggatggccat ccagcaggag gccctccttt ccggcctggt agagcctggg
     2161 gatgagattg accagggtgg atttacccga tccggtggcg ccgacgatgg cgaccgttgt
     2221 gcctgcgggg atgtgcaggg agatctcctt gaggacctgc ggcgccccct cggaataggc
     2281 gaaggagagg ctgcggaact cgatctcgcc ctgcagctcc gtgatactat agtcggtttc
     2341 gggaccatcc gcgacctccg gcttttccct gaagatcgcc aggatgcgct cggtggaggc
     2401 gagcccctct ttccacatgt tgagagtcca gcctacggcg atcatcggcc aggccagcat
     2461 ggcgaggtag gagagaaagg cgaccaggcc gccgatgctg agtcggccgg ccatgacctc
     2521 ccgcccgccg gcgaagaggg ccgccagggt ggtcagtccg aggagaaaat cgatgctcga
     2581 ccacatcccg gaacgcacca ggaccagctt gaggttgcgg cggatatatt cgcggctgat
     2641 ggcgcggaaa tcatcgatct catgcccctc ctggacgtag gatttgacca cccggatgcc
     2701 ggagatgttc tcctgcacct tggctgtgag ggtggaaaac tgctcctgga tgcggtcgaa
     2761 gagtttttcg atttgtccca cctggcggcc gacgatgatg gccaccaggg gcatgaggag
     2821 cagagcccag agggtcgtcc gccaagagag ggtggccatc agcgcgatcg agatgatggc
     2881 ggtgacgaag gtggtggcga agtacatgat cccagggccc accatggagc gcaccgcctc
     2941 catgtcgttg gtcgcccggg ccataagatc cccggtcttg tggcgctgga aaaagcccgc
     3001 cgagaggcgc tggagatggg cgagatagtc gttgcggagg gcatattcga cccgccgcga
     3061 catgccgatc agctgcatgc gcagatggta gcggaagatg ccctggaaaa ggctaatcac
     3121 aaccatggca agggcaaaga ggccgaggat gagcaagggg gtatggccct cggtgagagc
     3181 gaggatccag gccggcagcg cggcgccggc gctgcccaga cgccattccg catactcgat
     3241 ggcatagcgc agtaccatgg gtgaggccag ctggaagcca ctggtcagca gggtgagcag
     3301 cagacccagc atcagagtcc ccttgtgctg ttccaggtag tggagaatga agcgcagtgg
     3361 tttcccggtg ggcggcatta tggttggagc catcattttc cggtcagggt taggggaaga
     3421 cagcgcccga cggctgtaac atcccggccg ggcctgcgta tgattagtcg gattttgctc
     3481 actcaatata gctaattaca gtgagaatga caagaatttc gctggtctca gtggaagatg
     3541 gggaaccagt ttg
//
LOCUS       DAOPWU010000343         3545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_26865_length_3545_cov_3.285100, whole genome shotgun sequence.
ACCESSION   DAOPWU010000343 DAOPWU010000000
VERSION     DAOPWU010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26865_length_3545_cov_3.285100"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /gene="pruA"
                     /locus_tag="PKM23_13380"
     CDS             <1..1354
                     /gene="pruA"
                     /locus_tag="PKM23_13380"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNY92509.1"
                     /translation="FHKAGQAEVDLAMKAALEAFEKWRWVAPKERAIILLKAAAVMRR
                     RKLEVNAWMITEIGKSWPEADADTSEAIDFLEFYAREALRWSEVKGATPFPNEYPELN
                     YIPLGVGAIIPPWNFPMAILTGMTAAAIVTGNTVLLKPASDTPCIGYLLVEIMREAGL
                     PAGVLNFIPGSGGEIGDYIVTHPKTRFISFTGSKEVGLRIINLASQVSPGQIWVKRVV
                     AEMGGKDTIVVDADADLQEAATAIAASAFGFQGQKCSACSRAIVVEEVYDRIIALLTE
                     RVKKLTVGPPEDPSTYMGPVANQASEEKILGYIEIGKKDGRLICGGAKAPGEGYYIQP
                     TIFADIQPGTPLDQEEIFGPVLAVIKAKNFEEAIRIANSTEFGLTGAVWTRDRYKIEE
                     AKKKFHVGNLYVNRKCTGAMVGAHPFGGFNMSGTDSKAGGMDYLGLFMQAKSVSERLI
                     "
     gene            1531..2475
                     /locus_tag="PKM23_13385"
     CDS             1531..2475
                     /locus_tag="PKM23_13385"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNY92510.1"
                     /translation="MQKEPLYTIRTLALKSGVSMHTIRAWEKRYQALKPRRTPGNRRL
                     YTGEDLARLQLLRQGVADGHTISLLAGLPTPDLWRLVHGRSSIPAEPETMEHSHAVEA
                     LLENSWRAVQNLDDRALQKNLHQAAALLSQPVLLEQLLAPLLQRIGHGWSEGRLRILH
                     EHMATIVIRDFLTTLRLACTADPEAPVIIIATPAGQLHEMGALFVALTAAAQGWQIQY
                     LGTSLPADELAAAAHRLDARIIALSIIQPSDDPQLPLQLEILRNLAGSETILIAGGGA
                     AALFAPLLDRLSIHLINGIAQLREFLARERGLGQQPQP"
     gene            complement(2536..>3545)
                     /locus_tag="PKM23_13390"
     CDS             complement(2536..>3545)
                     /locus_tag="PKM23_13390"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HNY92511.1"
                     /translation="QPARLQVIHNAADPAAAAVDIYVNDALLLDNFAFRAATPYIDVP
                     SNTELRIGVAPGTSTSSAQALATFPVTLEPGKTYVVMASGVLNPAEFAVNPGGKNTGF
                     NLYINAAGRESAPAGEVMLSAFHGATDAPTVDVRVVGLVDPLVPALAFGDFSGYLSVA
                     PASYTLQITPAGVPGTVVASYAADLSGLGSGAAVVFASGFLALDQNKNGAAFGLFAAL
                     PNGTVIALPAPTLRKSAAALTGVEDGVVRVEGFSVQQNYPNPFNPVTRINFYLPGSEV
                     VQMQVFDVAGRQVSSMQMGELSAGAHSFSFDASGLPSGRYFYRIQAGAYSATKSMVLM
                     K"
BASE COUNT          729 a         1187 c         1013 g          616 t
ORIGIN      
        1 ctttcacaag gccggacagg ccgaggtcga tctcgccatg aaggccgccc tcgaagcctt
       61 tgaaaagtgg cgctgggtcg cacccaagga gcgcgccatc atcctgctca aggcggcggc
      121 ggtgatgcgc cggcgcaagc tcgaggtcaa cgcctggatg atcaccgaga tcggcaaaag
      181 ctggcccgag gccgacgccg acacctccga ggcgatcgat tttctcgaat tctacgcccg
      241 cgaggccctg cgctggagcg aggtcaaggg tgccactccc tttcccaacg agtaccccga
      301 actcaactac attcccctcg gcgtcggcgc catcatcccg ccctggaatt ttccgatggc
      361 catcctcacc ggcatgaccg ctgcggccat cgtcaccggc aataccgtgc tgctcaagcc
      421 cgcttccgac accccctgca tcggctatct gctcgtcgag atcatgcgcg aggcggggct
      481 gccggcgggc gtcctcaact tcatccccgg cagcggcggc gagatcggcg actatatcgt
      541 cacccatccc aaaacccgct tcatctcctt caccggctcc aaggaggtcg gtctgcgcat
      601 catcaacctg gcctcgcagg tttccccggg ccagatctgg gtcaaacgtg tggtcgccga
      661 gatgggcgga aaggacacca ttgtcgtcga tgccgacgcc gacctgcaag aagcggctac
      721 tgccatcgcc gcctccgcct tcggcttcca ggggcagaaa tgctcggcct gctcccgcgc
      781 catcgtggtc gaggaggtct atgacaggat catcgccctc ctcaccgagc gggtcaaaaa
      841 gctgaccgtc ggcccacccg aggatccctc cacctacatg ggacccgtcg cgaaccaggc
      901 ctcggaagaa aagatcctcg gctatatcga gatcggcaag aaggatggcc gcctcatctg
      961 cggcggggcc aaggccccgg gcgagggtta ttacatccag cctaccatat tcgccgacat
     1021 ccagccgggc acgcccctgg accaggagga gatctttggg ccggtgctgg cagtgatcaa
     1081 ggcgaagaat ttcgaggagg ccatccgcat cgccaacagc accgaattcg gcctcaccgg
     1141 cgcagtctgg acccgcgacc gctacaagat cgaggaggcc aagaaaaaat tccacgtcgg
     1201 caacctctac gtcaaccgca aatgcaccgg cgccatggtc ggagcccatc ccttcggcgg
     1261 ctttaacatg agcggcaccg actccaaggc cggcggcatg gactatctgg ggctgttcat
     1321 gcaggccaaa tcggtctcgg aacggctgat ctgatcgtct ctctctgcac ctcgccctgg
     1381 tcgggcggta taaccatgaa aaccagcaag gcgtgccctc cccggcacgc ctttttttgg
     1441 ggaagaggga gcaggccgac agcccaccgt gtgcatgcag catttcgcct tatttattga
     1501 caaattgtag acaaattcct atattttttc atgcaaaaag agcccctcta taccatccgc
     1561 accctggcgt tgaaaagtgg tgtgagcatg cataccatcc gcgcctggga aaagcgctat
     1621 caggccctga agccccgacg cactcccggc aaccgccgtc tctacaccgg agaggacctc
     1681 gcgcgtttgc agctcctccg gcagggggtc gccgacgggc acaccatcag cctgctcgcc
     1741 ggcctgccca cgcccgatct gtggcggctg gtgcacggcc gcagcagcat cccggcggag
     1801 cccgagacca tggagcactc gcatgccgtc gaggctctgc tcgagaacag ctggcgggcg
     1861 gtgcagaacc tcgacgaccg cgcactacag aaaaacctgc accaggcggc tgctctgctc
     1921 agtcagccgg tcctgctcga gcaactgctc gcccccctgc tccagcgcat aggccacggc
     1981 tggagcgaag gacggctgcg catcctgcat gaacacatgg ccaccatcgt catccgcgat
     2041 tttctcacca ccctgcggct cgcctgtacg gccgatccag aggctcccgt aatcatcatc
     2101 gccacccccg ccggtcagct ccacgaaatg ggcgccctct tcgtcgccct gaccgctgct
     2161 gctcagggct ggcaaatcca gtacctcggc accagcctcc ccgccgatga gctggctgca
     2221 gcagcgcaca gacttgatgc gcgcatcatc gccctcagca tcatccagcc ctcagacgat
     2281 ccgcagcttc ccctccagct ggagattctt cgtaatctgg ccggcagtga gacgatactg
     2341 atcgccggcg gcggcgctgc cgccctcttc gctcccctgc tcgaccgcct ctccatccat
     2401 cttatcaacg ggatcgccca gctccgcgag tttctcgccc gtgagcgagg cctcggccag
     2461 caaccgcagc cctgactcca caataaaaaa gccccgattc cttgcggtat cggggctttt
     2521 tttaaaatct gctgcttact tcatcaacac catcgatttg gtcgcgctgt aggctccggc
     2581 ctgaatgcgg tagaaataac gcccggaggg cagcccgctg gcgtcgaaac tgaatgagtg
     2641 agcgccggcg gagagctcgc ccatttgcat cgacgagacc tgacgtcctg cgacatcgaa
     2701 gacctgcatc tgcaccacct cactgccggg gagatagaaa ttgatgcggg tgacgggatt
     2761 gaagggattg ggatagttct gctggacaga aaatccctca acacggacaa ccccatcctc
     2821 gacacccgtc agagcggccg ccgacttgcg cagagtgggt gccggcagcg caattaccgt
     2881 gccattgggc agggcggcga aaagaccgaa agcggcaccg ttcttgttct ggtccagcgc
     2941 gagaaaaccg gaagcgaaga ctaccgctgc gccgctgccc agcccgctca aatcagcggc
     3001 gtaggaggca accacggttc caggcacacc ggccggggtg atctgaagag tgtagcttgc
     3061 cggagcgacc gaaaggtatc cgctgaaatc accgaaagcc agtgcaggga ccagaggatc
     3121 aacaagtccg accaccctca catctaccgt cggtgcatca gtagctccgt ggaaagcgct
     3181 cagcatcacc tcgcccgcgg gagcggactc gcgtccggcc gcattgatat agagattaaa
     3241 gccggtattc ttgccgcctg gattaaccgc aaattcggcc ggattgagca caccggaggc
     3301 catcacaacg taggtcttgc ccggttcaag ggtcaccgga aaggtggcca gagcctgagc
     3361 agaagaggtg ctggttccgg gggccacacc aatgcgcagt tcagtattcg agggaacatc
     3421 gatgtagggc gtcgctgcgc ggaaggcaaa attgtccagc agcagcgcat cgtttacata
     3481 gatgtccacg gctgccgcag caggatcggc cgcgttatgg atgacctgga gacgcgccgg
     3541 ctgtg
//
LOCUS       DAOPWU010000344         3544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_26874_length_3544_cov_4.404987, whole genome shotgun sequence.
ACCESSION   DAOPWU010000344 DAOPWU010000000
VERSION     DAOPWU010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26874_length_3544_cov_4.404987"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKM23_13395"
     CDS             <1..424
                     /locus_tag="PKM23_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNY92512.1"
                     /translation="VAAVIQKLFFPEWPEYFSFCFAAGISLAGVILGTYLTKPTEKKY
                     LENFYKKTRPFGFWGPIRTILPAQVLAKVNAENRRDIIATFFAVPWQIVLFLTMMMIV
                     MQRWDNFAWLLAALVGLSVGLYFTWFRHLSTEVKVDEK"
     gene            421..1677
                     /locus_tag="PKM23_13400"
     CDS             421..1677
                     /locus_tag="PKM23_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY92513.1"
                     /translation="MSPNAHKRGRLAVAALLLLMVLGLLAGCAPDRENRILVWHSMRP
                     IESDILRQALAEFALRHPEYVFDELFYAPETARTNFIISALGGSGPALFWGANDNIGP
                     LVELGVIKPLEEVYSKAFLDSFLTTPLPANTWMNGHLWQIADRIGNHLCLVYNKKLVP
                     RPPQTMNELIDFGKRYMAAHKGQQNYYPLVWNYTEPFFAVPFIGGYGGWVMDEKFQPT
                     LDQPAVVKAGKLIWELARVHKIIPLECDYEIANALFKDGYAAMIINGSWSWATYIENG
                     IDIGIARIPKIDETGLWPTPAVSPLGYSINVNLRGKKLEIARELLAWLTSAPLQLRFA
                     EVSGAIPSRVEAFQAPEVTANPLVSASLDQLLAGRIMPPVTEMRWIWDAMRPSYQGIF
                     TGRVTPEQAARDMQALALKLIKENRE"
     gene            1679..2572
                     /locus_tag="PKM23_13405"
     CDS             1679..2572
                     /locus_tag="PKM23_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY92514.1"
                     /translation="MAESAGQVKVNRLAYFYTMPAFFLMGLMIVYPFIYNVVISFSNM
                     NLSHFRDWHITGLGNYLQVVSGSSFWYFFFKTVLWTVLNVVFHVGIGVGLALLLNKDI
                     KGKSIFRTLLILPWAVPQYITALTWRGMFNSEYGAVNLLLDRALGLTIPWLTTEWGAF
                     AACLITNIWLGFPFMMIIALGGLQSIPDELYEAAEIDGSSWWHKLHHITIPLLRPVMV
                     PAITLGVIWTFNNFNVVWLVSNGGEPSDKTHILVSWIYKSAFTYFRMGYAAAFSMVLF
                     GILLLFSWNFIKKTRATEAVY"
     gene            2760..>3544
                     /locus_tag="PKM23_13410"
     CDS             2760..>3544
                     /locus_tag="PKM23_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY92515.1"
                     /translation="MRKPRDLSTTGKIAAYAVLILFTLFAIWPVLQVVSISLRPGDRL
                     LSKSLAIIPEDATLHSYVRLFTAEPFLRWLGNSVIVSGIVTLVGSLLAAFAGYAFSRY
                     KFRGRDTAMIGIITTQMFPVTMLLLPLFILLIKLKAYDNYLGLIIAYSATALPFTIWQ
                     MKGFYDTIPFSLEEAASIDGCTPFEVFWRIVMPLAAPALVINALFTFMTAWSEYLVAA
                     VIIQDKTMFTLPLGLKMFQSNMEVAWGLYSAGAVMVSLPVVLL"
BASE COUNT          656 a         1125 c          952 g          811 t
ORIGIN      
        1 ggttgccgcc gtcattcaga agctcttctt cccggaatgg ccggaatact tctctttctg
       61 cttcgctgcc gggatttccc tggccggagt catcctcggc acctatctga ccaaaccgac
      121 cgagaagaag tatctggaga atttttataa aaagacccgt cccttcggct tctggggccc
      181 catccgtact atcctgccag cgcaggtgct ggccaaggtc aatgccgaga accggcgcga
      241 catcatcgcc accttttttg ccgtgccctg gcagatcgtc ctttttctca ccatgatgat
      301 gatcgtcatg cagcgttggg acaatttcgc ctggctgctg gcggcgctgg tgggcctttc
      361 agtggggctc tattttacct ggttccgtca tctctccacc gaagtgaaag tggatgagaa
      421 atgagtccga atgcacacaa gagagggcgg ctggctgtgg ccgccctgct gctcctgatg
      481 gtgttggggc tgcttgccgg ctgcgcaccc gaccgcgaaa accgcatcct ggtctggcac
      541 tccatgcgtc cgatcgagag cgatatcctg cgccaggccc tggccgagtt cgctctccgt
      601 catccggaat atgtgtttga cgagctcttt tacgccccgg agaccgcccg caccaatttc
      661 atcatctccg ccctgggcgg cagcggtccg gccctcttct ggggagccaa cgacaatatc
      721 ggccccctgg tggaactcgg ggtgatcaag ccgctggagg aggtttattc aaaggccttt
      781 ctcgacagtt ttctcaccac acccctgccg gccaacacct ggatgaacgg ccatctctgg
      841 cagatcgccg accgcatcgg caaccacctc tgcctggtct acaataaaaa gctggtcccc
      901 aggccgccgc agaccatgaa cgaactgatc gactttggca agcgttacat ggccgcgcat
      961 aaagggcagc agaactatta tcccctggtc tggaactata ccgagccctt tttcgcagtc
     1021 cccttcatcg gcggttacgg gggctgggtg atggacgaga aattccagcc caccctcgat
     1081 cagccggcgg tggtcaaggc cggaaagctg atctgggagc tggcccgggt gcacaagatc
     1141 atccccctgg agtgcgacta tgagatcgcc aacgcccttt tcaaggacgg ttatgcggcg
     1201 atgatcatca acggcagctg gtcctgggcc acctatatcg agaacggcat tgatatcggc
     1261 atcgcccgca tccccaaaat cgacgagacc gggctgtggc cgacgccggc ggtttctccc
     1321 ctcggctact ccatcaatgt caacctgcgg gggaaaaagc tggaaatcgc acgcgaactg
     1381 ctcgcctggc tcacctcggc gcccctgcag ctccgctttg cagaggtttc gggggccatt
     1441 ccctcgcgcg tcgaagcctt tcaggccccg gaggtcaccg ccaatcccct ggtcagcgcc
     1501 tcgctcgacc agctgctggc cgggcggatt atgcccccgg tcacggagat gcgctggatc
     1561 tgggatgcaa tgcggccgag ctaccagggg atcttcaccg gccgggtcac gccggagcag
     1621 gcggccaggg atatgcaggc tttggctttg aaactgatca aggagaatcg ggagtaatat
     1681 ggcggagagc gccgggcaag tcaaggtgaa ccggctggct tatttttaca ccatgccggc
     1741 ctttttcctc atggggttga tgatcgtcta tcccttcatc tacaacgtcg tcatctcctt
     1801 ttcgaacatg aatctgagcc attttcgcga ctggcacatc accggactgg gcaactatct
     1861 ccaggtggtg agcggctcct ccttctggta tttctttttc aagaccgtgc tgtggacggt
     1921 gctcaacgtc gtcttccatg tcggcatcgg ggtcgggctc gccctgctcc tcaacaagga
     1981 catcaagggt aaatcgatct tccgcaccct gctcattctg ccctgggcgg tgccgcagta
     2041 catcactgca ctcacctggc gcggcatgtt caactccgag tacggtgcgg tcaatctcct
     2101 ccttgaccgc gccctgggcc tgaccatccc ctggctgacc accgaatggg gggccttcgc
     2161 cgcctgcctg atcaccaata tctggctcgg ctttcccttc atgatgatca tcgccctcgg
     2221 cggcctgcag agcatccccg acgaactcta tgaggcagcc gaaatcgacg gctcctcctg
     2281 gtggcacaag cttcaccaca tcaccatccc cctgctccgg ccggtgatgg tgccggccat
     2341 caccttgggg gtgatctgga ccttcaacaa tttcaatgtg gtctggctgg tctccaacgg
     2401 cggcgagccc tcggacaaga cccatatcct ggtctcctgg atctacaaga gcgcctttac
     2461 ctatttccgt atgggttatg ctgccgcctt ttcgatggtg ctcttcggga ttctgcttct
     2521 cttcagctgg aatttcatca aaaagacccg cgccaccgag gcggtgtact agttctctgg
     2581 ctaaatgaga gaaagaaaca agcaaaagcc atctctcacg gaggcacgga ggcacaaaga
     2641 agagcataag aatttatttc tttgtggctt ggtggcttgg tgagagtatt gttttctcta
     2701 cgttctctgc gtctcggcgc gagattgatt gtaatacgaa atatatttaa ggatacatga
     2761 tgcggaaacc gcgagacctc tcgacgacag gtaaaatagc cgcctacgcc gtgttgatcc
     2821 ttttcaccct cttcgccatc tggccggtgc tccaggtggt gtcgatatcc ctgcggcccg
     2881 gcgaccggct gttgagcaaa tccctggcga tcatccccga ggacgccacc ctgcactcct
     2941 atgtccgtct cttcaccgcc gagcccttcc tgcgctggct gggcaacagc gtgatcgtct
     3001 ccggcatcgt caccctggtg ggctcgcttc tggccgcctt tgccggctat gccttttcgc
     3061 gctacaagtt ccgcggccgc gacaccgcca tgatcggcat tattaccaca cagatgtttc
     3121 ccgtcaccat gctgctgctg cccctcttca tcctgctcat caagctcaag gcctacgaca
     3181 actatctcgg cctcatcatc gcctactccg ccacggcgct gcctttcacg atctggcaga
     3241 tgaagggctt ttacgacacc attcccttca gcctggaaga ggcggcgagc attgacggct
     3301 gcaccccctt cgaggttttt tggcgcatcg tcatgcccct ggctgcgccc gccctggtca
     3361 tcaacgccct cttcaccttc atgaccgcct ggagcgaata tctggtcgcc gcggtcatca
     3421 tccaggacaa gaccatgttc accctgccgc tcggcttgaa gatgttccag tccaatatgg
     3481 aggtggcctg gggcctctac tccgccggag cggtgatggt gagcctcccc gtggtcctgc
     3541 tttt
//
LOCUS       DAOPWU010000345         3530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_27013_length_3530_cov_5.300432, whole genome shotgun sequence.
ACCESSION   DAOPWU010000345 DAOPWU010000000
VERSION     DAOPWU010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27013_length_3530_cov_5.300432"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2463
                     /locus_tag="PKM23_13415"
     CDS             <1..2463
                     /locus_tag="PKM23_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY92516.1"
                     /translation="ARKYFHGDDMPDVSSTASYWLRPFFDDAVCWTGTTSGAWDTYTQ
                     SKYLEFEGWNAVSQQLCTDNDPNNDLTPLAAQRVFEYYTRKKQINDQPDYEIDGGFGG
                     PVPLVSKMLGNLRFFASYRGQKSLLIWPSSRPDYKDYDARVILNSDVSDAIKLRISGL
                     SGNISTMAGNRAVESTYKQWPYELAGTSLGTGGYELFNMFSDWTYGLAERKHNSLSAK
                     MTHMLSKSTYYELSAEYFHRNYDTHSPSMRDTSVTTEIIPGYFSTATPYGVFYGIDGI
                     VSGINEGNQNALARDKSNVSATTFKIDLTSQANFNNLVKAGAEFTYNDLDLDYGYIRM
                     QSGGMQYDNQIKMREFPIRAAFYLQDKLETKGFILNAGLRLDYSNSRTDWWDLDAFNP
                     YFYSYKYNENIDLATTGAKGQWQLSPRLGISHPITEKSKLYFNYGHFKQMPTYESQYR
                     FDRSTDNTLVRFGDPNLTLAKTIAYELGYDHELLDGQLLLQLAAFYRDISDQQNTTSY
                     RPAYGTAYTVTTSTAYSDIRGFELTLRKSAGRWFYGFLNYTYQATSNGAFGQNYVFED
                     PKSQQDYDENTVNIYQTRSVPSPYARANFTFATPRSYGPTLFNHAIFGDLLANILLRW
                     NQGGWTTYNPNNAGTTSFNNNTNNKIQNNVQYLDYYDATLRLSKSIQISKINLQVFAD
                     INNLFNTRRLTNTGDQNYRKSLHLPKSDAYNNIPGDDRFGDFREPGVEWQPMENGVDF
                     NSIPSSNRAWYYDKESNAYWYYMDDATIPVVQDRWAKVDQAKVDQAIEDKAYINMPNP
                     STYWFLNPRTITFGFRIAFDID"
     gene            2498..>3530
                     /locus_tag="PKM23_13420"
     CDS             2498..>3530
                     /locus_tag="PKM23_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92517.1"
                     /translation="MNNKKIKIQWLLVIGMILLAGSLHNAFAQDLEWIRVGRTQCFFM
                     DFGNECELYPFTETNFLAWPAQYGDNQYATRAKAVWMGATDFYDPVEKKTKSVKVIGS
                     GPRYDADNQAAMIFSTSIKLVGREEPPKVVVDNQSGSSNSLYDVLDEVDPNLPSDRMV
                     VIKYNTSMGVSVVKKVLGFVQQNHDSYFIHDYVFKNTGIYNSKGDVNPQTLTNFWVYF
                     SYRYAMNGVCSSGYSSAWGDFGMEWGSSTVAHDFGPPYRSRPDSLRGFYAFYSPIKGS
                     YGTRNAVTALGYDYDWGCPKQTGGGPNLDGLLGAAKYAGAVTLLASKGPGALFNVDDY
                     NQPATVNYFN"
BASE COUNT          858 a         1089 c          843 g          740 t
ORIGIN      
        1 gcacggaaat attttcacgg cgatgatatg cccgatgtca gctcgacggc atcgtactgg
       61 ttaagaccgt ttttcgatga tgcggtctgc tggaccggca caaccagcgg agcctgggat
      121 acttacaccc aatccaagta tcttgaattc gagggctgga acgccgtttc gcagcaactc
      181 tgcaccgaca acgacccaaa caacgatctc acgccgctcg cggcccaacg ggttttcgag
      241 tactacacgc gcaagaagca gatcaacgat cagccggatt atgaaatcga cgggggtttt
      301 ggcggaccag tacccctcgt ctccaagatg ctgggcaacc tgcgcttttt cgcctcctat
      361 cgtggacaga aaagcctgct gatctggccc agttcccggc ccgattacaa ggactatgac
      421 gcccgcgtta tcctcaactc cgacgtcagc gacgccatca agctgcgcat ctcaggcctt
      481 tccggcaata tcagcaccat ggccggcaac cgcgccgtcg aatcgaccta caaacagtgg
      541 ccttacgagc tcgccggcac ctcgctcggc accggcggtt atgagctctt caacatgttc
      601 agcgactgga cctacggtct ggccgaacgc aagcacaact ccctctccgc caagatgacc
      661 cacatgctga gcaaatcgac ctattatgaa ctctccgccg agtatttcca tcgcaactac
      721 gatacccact caccctctat gcgcgatacc tcggtgacca ccgagatcat tcccggctac
      781 ttttccaccg ccactcccta cggggtcttc tatggcatcg atggcattgt cagcggcatc
      841 aacgaaggca accagaacgc cctggccagg gacaaatcca atgtgagtgc cactaccttc
      901 aagatcgatt tgaccagtca ggccaatttc aacaacctgg tcaaggccgg cgctgaattc
      961 acctataacg acctcgacct ggactatggc tatatcagaa tgcaaagcgg cgggatgcag
     1021 tacgacaacc agatcaagat gcgcgaattc ccgatccggg ccgcctttta cctgcaggac
     1081 aaactggaaa ccaaaggatt tatcctcaat gcgggtctgc gcctggatta cagcaactcc
     1141 cgcacagact ggtgggatct ggatgccttc aacccctatt tctactccta caaatacaac
     1201 gaaaatatcg atctggccac caccggagcg aaggggcagt ggcagctcag cccgcgcctc
     1261 ggcatctccc atcccatcac cgaaaagtca aagctctatt tcaactatgg ccatttcaag
     1321 cagatgccga cctatgaatc ccagtaccgt tttgaccgct cgaccgacaa caccctggtc
     1381 cgtttcggcg atccaaacct gaccctggcc aagaccatcg cctacgagct tggctacgat
     1441 catgaactgc tggatggaca gctgctgctg cagcttgcgg ctttctatcg cgacatcagc
     1501 gatcagcaaa acaccacctc ctaccgcccg gcctatggga cggcttacac cgtcacgacc
     1561 agcacggcct attcggacat tcgcggattc gagctgaccc tgcgcaagtc cgccggacgc
     1621 tggttctacg gtttcctcaa ttacacctat caggcgacct cgaacggggc ttttggccag
     1681 aattatgtat tcgaagaccc caagtcacaa caggattacg acgaaaatac agtcaacatt
     1741 taccagaccc gctccgtgcc ctctccctat gcgcgggcca actttacttt cgccacaccg
     1801 cgcagctatg gaccgacgct cttcaatcat gcgatctttg gcgatctgct ggccaacatc
     1861 ttgctgcgct ggaaccaggg cggctggacg acctataacc ccaacaatgc cggcacgacc
     1921 agctttaaca acaacaccaa taacaagatc cagaacaacg tgcagtacct ggattactac
     1981 gatgccactc tgcgcctgag caagtccatt cagattagta aaatcaatct gcaggttttt
     2041 gccgacatca acaatctctt caacaccagg cgtctcacca acactggtga ccagaactat
     2101 cgcaagtccc tccacctgcc caaaagcgat gcctacaaca atattccggg tgacgacaga
     2161 ttcggcgatt tccgtgaacc gggcgtcgaa tggcagccca tggagaacgg cgtggatttt
     2221 aattccatcc cttcctccaa ccgcgcctgg tactatgaca aagagagcaa tgcctattgg
     2281 tattacatgg atgatgccac catcccggtt gtccaggaca ggtgggcgaa ggtcgaccag
     2341 gccaaggttg accaggccat tgaggataaa gcctacatca atatgcccaa tccctcaacc
     2401 tattggttcc tgaatccgcg aacgatcacc tttggcttcc gcatcgcatt tgatatcgat
     2461 tgatgtccgg caaccaacct aaagaatggg actcaagatg aataacaaga aaataaaaat
     2521 tcagtggctg ctggtgattg gcatgatcct gttggccggc agcttgcaca atgccttcgc
     2581 tcaggatctg gaatggattc gcgtggggcg cacacaatgc tttttcatgg actttggcaa
     2641 cgagtgcgaa ttgtatccct tcacagagac caattttctg gcctggcccg cccagtacgg
     2701 tgacaaccag tatgccaccc gggccaaggc cgtctggatg ggcgcaaccg acttttacga
     2761 tccggtcgaa aagaagacca aatcggtcaa ggtcatcggt tccggaccac gctatgatgc
     2821 ggataatcag gcggcaatga tcttttcgac cagcatcaag ctggtcggcc gggaagagcc
     2881 ccccaaggtg gttgtcgaca accagagcgg ttcctccaac agcctatatg atgttctcga
     2941 tgaggtcgat ccgaacctgc cgagcgaccg catggtggtc atcaaataca atacttccat
     3001 gggagtttca gtggtaaaaa aggttctcgg ctttgtccag cagaaccacg acagctattt
     3061 catccatgac tatgtcttca agaataccgg catctataac agcaaaggcg atgtcaatcc
     3121 ccagacgctc accaatttct gggtctattt cagctaccgc tacgctatga acggcgtctg
     3181 ctccagcggc tacagttcgg catggggcga ttttggcatg gagtggggct caagcacagt
     3241 ggctcatgac ttcggccccc cctaccgttc ccgtccggac agtctgcgcg gattctacgc
     3301 cttttacagc ccgatcaagg ggagctatgg cacccgtaat gccgtcaccg ccctgggcta
     3361 tgattacgac tggggctgcc cgaaacagac cggcggcggg cccaacctcg atggcctcct
     3421 gggggccgcc aaatatgccg gcgctgtgac tctgctcgcc tccaagggcc ccggcgctct
     3481 cttcaatgtc gacgactaca accagcccgc cacggtcaac tatttcaacg
//
LOCUS       DAOPWU010000346         3525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_27073_length_3525_cov_5.205764, whole genome shotgun sequence.
ACCESSION   DAOPWU010000346 DAOPWU010000000
VERSION     DAOPWU010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27073_length_3525_cov_5.205764"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKM23_13425"
     CDS             <1..555
                     /locus_tag="PKM23_13425"
                     /inference="COORDINATES: protein motif:HMM:NF037860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92518.1"
                     /translation="HMQGVMVRGHSLPNPRAAAVEPIVGEAIGRIAADLGYDAGPMNF
                     DAIIAGDSVYLLEIGLRNGGNGIVDLIYHGMGVDLLEMALDHALGEPLRTGWNGVQRP
                     VSSYVFGSGRPGRLRSIASLHALREMVPAVFDVVMARKPGEEVGAFTHNANLLGYLLL
                     DGGSAEYEILIAELEKHLHIEIEP"
     gene            552..1505
                     /locus_tag="PKM23_13430"
     CDS             552..1505
                     /locus_tag="PKM23_13430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY92519.1"
                     /translation="MKSIRYSIVIPCYSSGAWLAELAARIGQVMAPHSPFELILVNDH
                     SPDVQTWAAIEELAERYEFVRGIDLLYRVGQFKATLCGLELAVGNHVITMDDDLQHPP
                     EELVKLIEAMRDHPEMDCIMGVPASRGQSRLRHLGTLLMQTILGLFYGKPSGIVTTSF
                     RIMPAAFARTIVLYRMSFPQLGPLIVRLTHKIHNVPVEHRPRPLGSSGYGLFKLIKET
                     YRSIIHVTLLPLRIFSIAGIITAALAFFYGVYSFILWLAGGVGVPGFTSLILAIMFFG
                     GMLLVEVGILGEYIGRIILELTGMPRYAIYRQVGVRDEQDH"
     gene            1489..2013
                     /locus_tag="PKM23_13435"
     CDS             1489..2013
                     /locus_tag="PKM23_13435"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNY92520.1"
                     /translation="MNKTTEGRQLYLRRLEITDLERTWVWLHRREIYTKIGVKVPFSR
                     EEQLIWFEKLQQDPVKIVFAVCRLKDDAHIGNVSLDQIDSRHHNARMSIFIGDESLQG
                     QGCGSEAVQLLLEYAFSTLQLHKVWCKTDAGFPAVIRFYERLGFRQEGVLREHEFKEG
                     AFVDKVLLARINNP"
     gene            2118..3254
                     /gene="rffA"
                     /locus_tag="PKM23_13440"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             2118..3254
                     /gene="rffA"
                     /locus_tag="PKM23_13440"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNY92521.1"
                     /translation="MQDHPGIPFNKPFLSGRELDYIGQAVRNGRLSGNGPFTQKCQLF
                     FRERWRFNTSLLTTSCTDALEMAAILLDIQPGDEVIVPSFTFVSTANAFVLRGATIIF
                     ADSLAENPNLDVSALEALITPRTRAIVPVHYGGMACDMDPLIALARRHHIHIVEDAAQ
                     AINASYKGKALGSCGDLAAFSFHETKNIIAGEGGMLVVNNPEFAERAEIIWEKGTNRA
                     AFFRGEADKYHWVDIGSSFLPSELTAAFLWAQLENLEKIQARRLGQWNRYRNRLSAWA
                     QDQGVQIPQPSLHSSGNGHLFYLVFNTLQRRTQLIDMLCRHRISALFHYQSLHRSPFY
                     HNRHDGRSLPQAERYSDGLVRLPLFYELGDDKIDYICDLIVSQG"
     gene            complement(3243..>3525)
                     /locus_tag="PKM23_13445"
     CDS             complement(3243..>3525)
                     /locus_tag="PKM23_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92522.1"
                     /translation="VLGLPVEFMSKEHVTEYTVGQVLEQLRYAGMEAELFDEPLRGPA
                     GGLVRFIAFKMAVPIVRAMLQLIGSHHSPEETVFYLAHKQGWPAEGSSL"
BASE COUNT          722 a         1074 c          980 g          749 t
ORIGIN      
        1 cacatgcagg gtgtgatggt aaggggacat tccctgccga atccccgggc ggctgccgtc
       61 gagcccatcg tgggtgaagc gatcggccgt atcgccgccg atctcggcta tgacgctggt
      121 ccgatgaact ttgatgccat catcgcaggg gattccgttt atttgctgga aatcggtctc
      181 cgcaacggag gcaatgggat tgtagattta atatatcatg gcatgggtgt ggacctgctg
      241 gagatggcac tggaccatgc ccttggggag ccgctccgaa ccggctggaa tggagtgcag
      301 cgaccggtga gcagctatgt tttcggttcg ggccgtccgg gccggctgag atcgatcgcc
      361 tccctgcatg ccctgcggga aatggtgcct gctgtcttcg acgtggtgat ggccaggaaa
      421 ccgggcgagg aggtcggcgc cttcacgcac aacgccaacc tgctcggcta cctgctgctg
      481 gacggcggct cggctgaata tgagattcta atcgcggagc tggagaagca tctgcacatc
      541 gagatcgaac catgaaatca atccgctatt cgatcgtcat cccctgctac tcctccgggg
      601 cctggctggc tgaactggca gcccggatcg gacaggtgat ggcgccccat tcgccctttg
      661 aattaatcct ggtcaacgac cacagccccg atgtgcaaac ctgggcggca attgaggaac
      721 tggcggaaag gtacgaattt gtccgcggaa tcgatctcct ctatcgtgtc ggtcagttca
      781 aggcgaccct gtgcggcctg gaactggctg tcggaaacca cgtcataacc atggatgatg
      841 acctgcagca ccccccggag gaactcgtca agctgatcga ggcgatgcga gaccatccgg
      901 agatggactg catcatgggg gtgccggcct cccgagggca aagccggctg cgccatctcg
      961 gcaccctgct gatgcagacc atcctcggcc ttttttacgg caagccctcc ggcatcgtca
     1021 cgacgagttt ccgaattatg ccggccgcct tcgcccgcac gatcgtcctc tatcgtatga
     1081 gctttcccca actgggtccc ctgatcgtcc gactgaccca caaaatccat aatgtgccgg
     1141 tcgagcaccg cccacggccc ctcggcagca gcggctacgg actcttcaag ttgatcaagg
     1201 agacttatcg cagcatcatc catgtcacgc tgctgccact gcgcattttt tccatcgccg
     1261 gcatcatcac ggctgcactc gcttttttct acggagtcta ctccttcata ctttggctgg
     1321 ccggcggcgt cggcgttccg ggattcactt cgctgattct ggcgatcatg ttcttcggcg
     1381 gcatgctgct ggtcgaggtc gggatcctcg gggagtacat cggccgcatc attctggagc
     1441 taaccggcat gcccaggtat gcaatctacc gtcaggtcgg ggtgagggat gaacaagacc
     1501 actgaaggcc gacagctcta tctgcgcaga ctggagatca ctgatctgga acgtacctgg
     1561 gtctggctgc accgcaggga aatatatacc aagatcggtg tcaaggtccc cttctccagg
     1621 gaggagcagc tgatctggtt cgaaaaattg cagcaggatc ccgtcaagat tgtctttgcc
     1681 gtctgccgtc tgaaggatga cgcccatatc ggcaatgtct cgcttgacca gatcgacagc
     1741 cgccatcaca acgccaggat gagcatcttc atcggagatg agagcctgca gggccagggc
     1801 tgtggctcag aggctgtcca gcttttactg gagtatgcct tctcaaccct ccaactgcac
     1861 aaggtctggt gcaaaaccga cgcaggtttc cccgcagtga tccgttttta tgaaaggctg
     1921 ggtttcaggc aggaaggagt gctcagggag catgaattca aggagggcgc ctttgtcgac
     1981 aaggtgctgc tggcccggat caacaacccc tgagccgcag ggatccgccc gccgattttt
     2041 ccgcgattta cagccttatt gcctacttgt atatataccc aagagattca tttagttaac
     2101 actaaaggct gacaagcgtg caggatcatc ccggcatccc tttcaacaag ccctttctct
     2161 caggcaggga gctcgactat atcgggcagg ccgtgcggaa cggccggctc tcgggcaacg
     2221 gccctttcac acaaaaatgc cagctcttct tcagggagag atggcgtttc aacacaagcc
     2281 tgctgaccac ctcctgtacc gatgctttgg agatggcggc gattctgctc gatattcaac
     2341 cgggcgacga agtgatcgtg ccctcgttca cctttgtctc cacggccaac gcctttgtct
     2401 tgcggggcgc caccattatc tttgccgaca gtctggctga aaatccaaac ctggacgtca
     2461 gcgccctcga agccctgatc actcccagga cgcgcgcgat cgtgccggtg cactatggcg
     2521 gcatggcctg cgatatggat cccctcatcg cactggcccg ccgccatcac atccatatcg
     2581 tcgaggacgc agcccaggcg atcaatgcct cctacaaagg caaagccctg ggctcctgtg
     2641 gagatctggc ggcattttct ttccacgaga ccaaaaacat catcgccgga gagggcggca
     2701 tgctggtcgt caacaatccc gagtttgcag agcgggcgga gatcatctgg gaaaagggga
     2761 ccaaccgtgc agctttcttc cggggtgagg ctgataaata ccactgggtg gatatcggtt
     2821 cctctttcct gccgtcggag ctgactgccg cttttctttg ggcacagctc gaaaacctgg
     2881 agaagattca ggccagacgc ctcggacagt ggaaccggta caggaacagg ctctcagcct
     2941 gggcgcagga tcagggagtg cagattcccc aaccgtccct ccattccagc ggcaatggcc
     3001 atctcttcta tctggtattc aacacactcc aacgccgcac ccagctgatt gacatgctgt
     3061 gcagacaccg gatttcggca ctctttcact atcaaagcct gcacaggagc cccttctacc
     3121 acaaccgcca tgacgggcgt tccctgcccc aggcggaacg ctattccgac ggtctcgtgc
     3181 gcctgccgct cttttacgag ctcggcgatg acaagatcga ctatatctgc gacctcatcg
     3241 tttcacaagg atgatccctc cgccggccag ccctgcttgt gcgcaagata aaaaaccgtc
     3301 tcctcgggac tgtgatgcga tccgatgagc tgcagcatgg cgcggactat cggcaccgcc
     3361 atcttgaagg cgatgaaacg caccaggccg ccggccggac cccgcagcgg ctcatcgaaa
     3421 agttccgcct ccatcccggc gtaacgcagc tgctccagca cctgtcccac tgtgtactcg
     3481 gtcacatgct ccttcgacat gaattctacc ggcaggccca ggacc
//
LOCUS       DAOPWU010000347         3517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_27155_length_3517_cov_4.908723, whole genome shotgun sequence.
ACCESSION   DAOPWU010000347 DAOPWU010000000
VERSION     DAOPWU010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27155_length_3517_cov_4.908723"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3479)
                     /gene="sprA"
                     /locus_tag="PKM23_13450"
     CDS             complement(<1..3479)
                     /gene="sprA"
                     /locus_tag="PKM23_13450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNY92523.1"
                     /translation="MVIFVSLVGQVWADDAQPAPYIGMRPPAPVSPLVPIFEEQNYRG
                     LNLQGFTSPPLKRQVAGIRRESKVDSTGRNINFYENYDKIPVSLPNFLSLQRYAEMRR
                     QIGLGNLWQRNAIAKLGETASGGGGGALRIDIPVEIKSKAFQKIFGSGTVGLDVTGDI
                     SIKGGLRHEKHSEVKTALNQSPDTNFKMEQTQRFKVQGHIGDKVTIGVDQDSDRTFDF
                     ENSLKLKYQGYDDEIIQTIEAGNIALSLPGTRYVTFGGQSSGLFGIKTAMRMGNLSLT
                     AIASQEKGEKKQLSLKGGASEGVKEIQDYDYLEGYYYFIDDYYRKQFPVRGPNGEFLV
                     DPSRRISRIEVYKSRYEYSRTSGESVISGWAWVPDAADSSTLVITEGDTTFQSSENYR
                     GPFIRQEKTEYYFDPELGYLRMNQPLDDSEVLAVAFRDTSGYTRGSIDFDPDTDTVVQ
                     LRLLKPMSPRPSNKTWDLAWRNVYMIGPKNMSDDGFELRILFKPASGDPQETIAVDGK
                     TVTYLQVFGLDRVNKNGDPTPDNIIDRNDNILRLGQGELWLPSLRPFDPGEAEFQAKL
                     PREKRSPAIYDTTVQQVINGQSKFYFEVKSKTRSAEYSLGMNVIENSEEVILNGRRLT
                     RGTDYIIEYFTGSLKMLNQEALQPSANVEISYESNQIFQIDKKTVMGARAEYGLWDDS
                     FIGATLLYLNESTLDQKVRVGKGPMRNLVWDVNTAMTLKPFFLTRAANLLPFVDSRDP
                     TTIKFEGELAQVIPNPNTRNNDDTGDHDGVAYIDDFEAAKRITPLGITRKSWRPAAPP
                     IGKVDPRLIYPTLEARGTMKWYEPYGQVSIKEIWPNRDINANVAQTVNVLIMDFEPAD
                     SLADITQSWGGLQKGLSSGYYDQSESKFLEIWVKGTQGTMHIDIGQISEDYIPNSKYD
                     SEDKMRNGFRNNLLDEGEDIGVDGMANSDPRAIGGGGDFWDINGNGIRDFGEPYSNDD
                     WAYSSGSADYDKINGTEGNENDTGGRVPDSEDMNGNYALDVNNEYFSYSFSLERDHPD
                     TVYIAGASINKETGEDNGWRLYRIPLDADAPTLTKQGSPDITRIEYIRVWVDGFKSSG
                     LHRVWLAELDLVGSEWKELGVADALNPENYQSVADSVVTVSVVNTHDNPDYIAPPGVS
                     GETDRITGVIARE"
BASE COUNT          611 a          989 c         1144 g          773 t
ORIGIN      
        1 tgttcgcgcg cgatgacgcc ggtgatacgg tcggtctcgc ccgaaacccc cgggggagcg
       61 atatagtcgg ggttgtcgtg ggtgttgacg accgagaccg tcacgaccga gtcggcgacc
      121 gactgatagt tctcagggtt cagggcgtca gcgacgccga gctctttcca ctcgctgccg
      181 accagatcca gttcggccaa ccagacgcgg tgtaaaccgg aactcttgaa accgtccacc
      241 cacacccgga tatactcgat gcgggtgatg tcgggcgaac cctgcttggt cagggtcggc
      301 gcgtcggcat ccagggggat gcgatagagg cgccagccgt tgtcttcgcc ggtctccttg
      361 ttgatggagg cgccggcgat atagactgtg tcggggtgat cgcgctcgag cgagaaggaa
      421 taggagaaat actcgttatt gacatcgagg gcgtagttgc cgttcatgtc ctcgctgtcg
      481 ggaacgcggc cgccggtatc gttctcgttg ccctcagtgc cgttgatctt gtcataatcg
      541 gcgcttcccg agctgtaggc ccagtcgtca ttgctgtagg gttcgccgaa atcccggatg
      601 ccgttgccgt tgatgtccca gaaatcccct ccacctccga ttgcccgcgg atcggagttg
      661 gccatgccgt cgacgccgat atcctcccct tcatcgagga ggttgttgcg aaaaccgttg
      721 cgcatcttgt cttcgctgtc gtacttgctg ttggggatat agtcctcaga gatctggccg
      781 atatcgatat gcatggtgcc ctgggtgccc ttgacccaga tctcgagaaa cttggattcg
      841 gactgatcat agtagccgct cgaaagcccc ttttgcaggc cgccccagga ttgggtgatg
      901 tcggcgaggc tgtcggcggg ctcaaagtcc atgatcagca cattgaccgt ctgggcgacg
      961 ttggcgttga tgtcccggtt cggccagatc tccttgatcg atacctgacc gtagggctcg
     1021 taccatttca tcgtgccgcg tgcctcgagg gtcggataga tcagccgggg atcaaccttg
     1081 ccgatcggcg gcgcagcggg ccgccagctt ttacgggtga tgcccagggg cgtgatacgc
     1141 ttggccgcct caaagtcgtc gatgtaggcg accccgtcgt gatcgccggt gtcgtcgttg
     1201 ttgcgggtgt tgggattcgg gatcacctgc gccagctcgc cttcgaactt gatggtggtc
     1261 gggtcgcggg aatcgacgaa gggaagcaga ttggcggcac gggtcaggaa gaagggcttg
     1321 agcgtcatcg ccgtgttgac gtcccagacc agattgcgca tcggtccctt gcccacgcgc
     1381 accttctggt cgagggtgct ctcgttgaga tagagcaagg tggcgccgat gaagctgtca
     1441 tcccagaggc cgtactcggc gcgcgccccc atgacggtct ttttgtcgat ctggaagatc
     1501 tggttgctct cgtagctgat ctcgacattg gccgagggct gcaaggcctc ctggttgagc
     1561 atcttgagac tgccggtgaa gtactcgatg atatagtcgg tgccgcgcgt gagcctgcgg
     1621 ccgttgagga tgacctcttc cgagttctca atgacgttca tgccgaggga atactcggcg
     1681 ctgcgggtct tggatttgac ctcgaaatag aacttgctct ggccgttgat gacctgctgc
     1741 acggtggtgt cgtatatagc gggagagcgc ttctccctgg ggagtttggc ctgaaattcg
     1801 gcctccccgg ggtcgaaagg ccgcaggctg ggcagccaga gttcgccctg gccgagcctg
     1861 aggatgttgt cgttgcggtc gatgatattg tcgggggtgg gatcgccgtt cttgttgacg
     1921 cgatcgagcc cgaagacctg cagataggtc acggtcttgc catccaccgc gatggtctcc
     1981 tggggatcgc cggaggccgg cttgaagagg atgcgcagct caaagccgtc atccgacatg
     2041 ttcttggggc cgatcatgta gacattgcgc caggccagat cccaggtctt gttgctcggc
     2101 ctgggactca tcggcttgag caaacgcagt tgcacgactg tatcggtgtc cggatcgaaa
     2161 tcgatgctgc cgcgggtgta gcccgaggtg tcgcgaaagg ccacggccag gacctcggaa
     2221 tcgtccaggg gctgattcat gcgcagataa cccagctccg gatcgaaata gtattcggtt
     2281 ttttcctggc ggatgaaggg accgcggtaa ttctccgaac tctggaaggt ggtgtccccc
     2341 tcggtgatga ccagggtgct cgaatcggcc gcgtcaggca cccaggccca gccgctgatg
     2401 acggactcgc cgctggtgcg gctgtactcg tagcgggatt tgtagacctc gatgcgggat
     2461 atgcggcgcg agggatcgac aagaaactcg ccgttgggcc cgcgcaccgg aaactgcttg
     2521 cggtaataat cgtcaatgaa gtagtagtag ccttcgagat agtcgtaatc ctggatctcc
     2581 ttgaccccct cggaggcgcc gcccttcagg gagagctgct tcttttcacc cttctcctgg
     2641 ctggcgatgg cggtgaggga gagattgccc atccgcatcg cggtcttgat gccgaagagg
     2701 ccggagctct ggccgccgaa ggtgacgtaa cgggtgccgg gcagggagag ggcgatgttg
     2761 ccggcctcaa tggtctggat gatctcgtcg tcatagccct gatacttgag cttgaggctg
     2821 ttctcgaaat caaaggtgcg gtcggaatcc tggtcgaccc caatggtcac cttgtcgccg
     2881 atgtgcccct gcaccttgaa gcgctgggtc tgctccatct tgaaattggt gtcgggactc
     2941 tggttgaggg cggtcttgac ctcgctgtgc ttctcatggc gcagcccccc cttgatgctg
     3001 atatcgccgg tgacgtcgag accaaccgtg ccgctgccga agatcttctg gaaggccttg
     3061 ctcttgatct ccaccgggat gtcgatgcgc agcgctccgc cgccgcctcc gctcgcggtc
     3121 tcgcccagct tggcaatggc gttgcgctgc cagaggttgc ccaggccgat ctgacgacgc
     3181 atctcggcgt agcgctgcag gctgaggaaa ttcggcaggg aaaccgggat cttgtcgtag
     3241 ttttcgtaga aattgatgtt gcggccagtg ctgtcgacct tgctctcgcg gcggatgccg
     3301 gcgacctgcc gcttcagggg cggcgaagtg aaaccctgca ggttgagacc gcggtagttc
     3361 tgctcctcga agatgggcac aaggggggag acgggcgcag gcggacgcat gccgatatag
     3421 ggcgcaggct gggcatcatc tgcccatact tgtcccacca ggctgacgaa aatgaccatc
     3481 gaaagcacca ccgcaggcgg cagggtgacc atcgatg
//
LOCUS       DAOPWU010000348         3505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_27283_length_3505_cov_4.705217, whole genome shotgun sequence.
ACCESSION   DAOPWU010000348 DAOPWU010000000
VERSION     DAOPWU010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27283_length_3505_cov_4.705217"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1094)
                     /locus_tag="PKM23_13455"
     CDS             complement(<1..>1094)
                     /locus_tag="PKM23_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007103736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNY92524.1"
                     /translation="PWGSDWYELQPWEKATGRDIWFNIQRRRYGGDLAGILEKLDYIQ
                     ELGVTALYLNPVFASPSLHKYDGATYHHIDPNFGPDPAGDRAIIAGENPVDPAAWQWT
                     AADRMMLELIREVHRRGMRIIFDGVFNHMGINSFAFQDLIAHGQASPYRDWFTITSWT
                     KPSRHAPFSYAGWANVVELPELREDENGIVAGPREYIFASTRRWMDPDGDGDPGDGID
                     GWRLDVAFCVAHPFWQAWRTHVKTINPEAYLTAEVVDKIKVLQPYLRGDEFDAVMNYN
                     FAFSCIEFFVAEKTRISVAEFDRRLAELRAAFPAGTAFVMQNLLDSHDTMRLGSLIVN
                     RDRGSIRDWGKFFDLSKGSHPNTATRKPGL"
     assembly_gap    1095..1194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1257..3458)
                     /locus_tag="PKM23_13460"
     CDS             complement(1257..3458)
                     /locus_tag="PKM23_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY92525.1"
                     /translation="MTLIPKAAVRIRPQRSAALMLLGLLLLPALLSAQATLRGRVTDQ
                     ENGQPLPGANIQVTGTLRGAVADANGRYLVTQVPAGIYTVRVSMMGYRSRTIDKVSLT
                     EGRETPLDVKLRPTPIEMDPVIISAGKTQQSLDQAPVSLSVVSSLEIKQRSATNIQEA
                     LESAPGVHFVGSQINIRGSTGYTFGAGNKVLLLLDGVPVYASDTGEFNWDMLPPLDIE
                     QIEVVKGAGSTLWGASALGGVVNVLTKDPDPEGRLLFSYTAARYDKPLYEEWQWTDPN
                     RLHYTREDLSYSRRIGRFGGRLSLGRFMTTGYTQIGDAVKYNLTGKFDYQWESGVKWT
                     VYSAYSHISRGFFVQWKGPNDPYEVDPSNLGNHAKTNQLNSYTKLVVPFSPTFAMNFR
                     ASLVRTLMGNQFGPSGNFNPAYGEGLEVQADWIPQFNHTVTAGVQFQHDAGSTKYFGS
                     HKGYFIGPFLQDEWKIRPNLRSTLGFRYDRYQLVGGLKEDLFSPRIGVNWQPWSKTSI
                     RASAGSGFRAATIIERFLELAIMNFKIVANPALKAESSWAYDLGVRQYINENWNLDLS
                     LFDNEYWELIEAHMDLIRGQVQFRNIPRARIRGIEATTNFSRPFTFRSWPVTPSLQLT
                     ATAMDHEELEYHDPLPYRPKLIASGKAGLRIARAQIQVEYRHASRIEAVKIYPINDRV
                     PMKFWDVRLSYDWRGLSFKLGALNVGQYNYAPMESNLMPMRTYTVGLQGEF"
BASE COUNT          603 a          978 c         1174 g          650 t
ORIGIN      
        1 tccaatcctg gtttgcgcgt ggcggtgttc ggatggctgc ccttgctgag gtcaaaaaat
       61 ttgccccagt cgcggatcga gccgcggtca cggttgacga tgagcgagcc gaggcgcatg
      121 gtgtcgtggc tgtcgagcag attctgcatg acaaaggcgg tgcccgccgg aaaagcggcg
      181 cgcagctcgg ccaaacggcg gtcgaattcg gccacgctga tgcgcgtttt ttcagccacg
      241 aagaactcga tgcaggaaaa ggcgaaatta tagttcatca ccgcatcgaa ctcgtcgccg
      301 cgcaggtaag gctggagcac cttgatcttg tcgacgacct cggcggtgag ataggcctcg
      361 ggattgatcg tcttgacgtg ggtgcgccag gcctgccaga aaggatgggc gacgcaaaag
      421 gcgacatcca gacgccagcc gtcgatgccg tcgcccggat cgccgtcccc gtcggggtcc
      481 atccagcgcc gtgtggacgc aaagatgtac tcccgcgggc ccgcgacgat gccattttca
      541 tcctcgcgca gctcgggcag ctcgacaaca ttggcccagc ccgcataact gaagggggcg
      601 tggcgggaag gttttgtcca cgaggtgatg gtgaaccagt cgcggtaggg cgaggcctgg
      661 ccgtgggcga tgagatcctg aaaggcgaag ctgttgatcc ccatgtggtt aaaaaccccg
      721 tcaaagatga tgcgcatgcc gcggcggtgg acctcgcgga tgagctcgag catcatccgg
      781 tcagcggccg tccactgcca ggcggcggga tcgacgggat tctctccagc gatgatcgcc
      841 cggtccccgg cgggatcggg cccgaaattg ggatcgatgt ggtgataggt ggccccgtca
      901 tatttgtgca gcgaggggga ggcgaaaacc ggattgaggt agagggcggt gacgcccagt
      961 tcctgaatat aatcaagttt ttccaggatg ccggcgaggt cgccgccgta gcgccgccgc
     1021 tggatattga accagatgtc gcggccggtg gccttttccc agggctgcag ctcgtaccag
     1081 tcggagcccc agggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntggttt
     1201 catggcgtca cctgttatgt tggctgatcg cctgcattgg gcacaagacc ggttggctaa
     1261 aattctccct gcagccccac ggtataggtt cgcattggca tcagattgct ctccatcggg
     1321 gcatagttgt actgcccgac gttgagggcg ccgagcttga agctcaggcc gcgccagtca
     1381 tagctcaacc gcacatccca gaatttcatc ggcacccggt cattgatcgg gtagatcttg
     1441 accgcctcga tgcggctggc gtggcggtat tcgacctgga tctgggccct ggcgatgcgc
     1501 agaccggcct tgccggaggc gatcaacttc ggccggtagg gcagcggatc gtggtactcc
     1561 agctcctcat ggtccatcgc cgtggcggtg agctgcaggc tcggggtgac cggccagctg
     1621 cggaaggtga aaggccggct gaaattggtg gttgcctcga tgccgcggat gcgcgcccgc
     1681 gggatgttgc gaaactgcac ctggccgcgg atgaggtcca tgtgggcctc gatcagctcc
     1741 cagtactcgt tgtcgaagag ggagaggtcg aggttccagt tctcgttgat gtactgccgc
     1801 accccgaggt cataagccca ggagctctcc gccttgaggg cgggattggc gacgatcttg
     1861 aagttcatga tcgccagctc gagaaagcgc tcgatgatgg tggcggcgcg gaagccgctg
     1921 cccgccgagg cgcggatgct ggttttgctc cagggctgcc agttgacgcc gatgcgcggc
     1981 gagaagaggt cctccttgag gccgccgacc agctggtagc ggtcatagcg gaagcccagg
     2041 gtcgagcgca gattgggccg gatcttccac tcatcctgga gaaaggggcc gatgaaatag
     2101 cccttgtggg agccgaagta tttggttgag ccggcgtcgt gctggaactg caccccggca
     2161 gtgacggtgt ggttgaactg cggaatccag tctgcctgca cctcgagccc ctcgccgtag
     2221 gcgggattga aattaccgct cgggccgaac tggttgccca tcagggtgcg caccagcgag
     2281 gcgcggaaat tcatcgcaaa ggtcggcgaa aaggggacga ctagcttggt gtagctgttg
     2341 agctgattgg ttttggcatg gttgccgagg ttgctgggat cgacctcata gggatcattc
     2401 ggtcctttcc actgcacgaa aaagccgcgg ctgatgtggc tgtaggcgct gtagacggtc
     2461 cacttgacgc cgctctccca ctgatagtcg aacttgccgg tgaggttgta cttgaccgca
     2521 tccccgatct gggtgtagcc ggtggtcatg aagcggccga gggagaggcg cccgccgaaa
     2581 cggccgatgc ggcggctgta actgaggtcc tcgcgggtat agtgcaaccg gttgggatcg
     2641 gtccactgcc actcctcata gaggggcttg tcgtatctgg cggcagtgta ggaaaagagc
     2701 aggcggccct ccggatcggg atccttggtg aggacgttga ccacaccacc gagggccgag
     2761 gccccccaga gggtggagcc ggcccccttg accacctcga tctgttcgat gtccaggggc
     2821 gggagcatgt cccagttgaa ttcgccggtg tcgctggcgt agaccggcac gccgtcgagc
     2881 aggagcagca ccttgttgcc ggctccgaag gtgtagccgg ttgagccgcg gatattgatc
     2941 tggctgccga caaaatggac cccgggagcg gactcgagcg cctcctggat attggtggcg
     3001 ctgcgctgct tgatttcgag cgaggagacc accgagagcg agaccggggc ctggtcgagc
     3061 gactgctggg tcttgccggc ggagatgatc accgggtcca tctcgatcgg ggtcggccgc
     3121 agcttgacat cgaggggggt ctcgcggccc tccgtcaggc tgaccttgtc aatggtgcgg
     3181 ctgcggtagc ccatcatcga tacccgcacc gtgtagatgc cggcgggcac ctgggtgacc
     3241 agatagcggc cgttggcgtc ggcgaccgcg ccgcgcaggg tgcccgtcac ctggatgttg
     3301 gctcccggca ggggctggcc gttctcctgg tcggtgaccc ggccgcgcag ggtggcctgg
     3361 gctgagagca gggcggggag cagaagcagg ccaagcagca tgagagccgc agacctctgc
     3421 gggcggatac ggacagccgc tttagggatc agcgtcatca ctccctcccc tctttcccgg
     3481 ccgcactcca gaccgcctgt tccgc
//
LOCUS       DAOPWU010000349         3504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_27298_length_3504_cov_5.270803, whole genome shotgun sequence.
ACCESSION   DAOPWU010000349 DAOPWU010000000
VERSION     DAOPWU010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27298_length_3504_cov_5.270803"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..3504
                     /locus_tag="PKM23_13465"
     CDS             142..3504
                     /locus_tag="PKM23_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4957 domain-containing protein"
                     /protein_id="HNY92526.1"
                     /translation="MLITVSSLFAQTIHQVPAGSDVISTAYESAAPGDIIELTTDGGE
                     YIEGNDLVIIKDKPVTIRAASGLTTKPRWSASGGWALLQTADDLTLEGIYVDGTVAGG
                     AREVGIRGAEPEWYALQLGYTLKLLNCDFVNFDYAVYGEDSNQLDSLIIDGCTFSHII
                     KQAIQFSDGTIAPGQVKHFFCTNSTFWDIGGYAIYLQSSAIDANPQAEFVVDHVTIHD
                     AANGNIYPRDIDGAVIKNTIITNSGGSPGSVCIIAGLNSRISNLLYNGLDQVSLEAGA
                     VESQLTSVWAGVDPLYASAVSGNFTLMTGSPAIGAGDDGKSLGDPRWWPENNSEYVFV
                     FAGENTLADAVASAVAGQTIMLVSDGGLYDNTHKMVIDKELTIRSADGLKQRPLLTSG
                     ASDAIFEIGAGFSLEGVILDGALGADSTAIGITNAPNTTGYNLKVLKTDFLNFSDKGQ
                     TTGFGIFGVPSSVVDTVLIQDCYFAHILDMGISFNDPLTATGSVNVFKVDNCTFWDMN
                     SEAIYIDAFDSKMETKDPEVTINKVTVYDCGSYNLIAHYIDYSVLTNSMVVLPELNTS
                     YAPTKVYGTNSKVENFLYFNTRDIDLSYGATDFQLVNIVPQGDPGLVDPENGDFSYTV
                     HSPAVFFLEDGDFLRLGDDRWWPGINLPANIHWGTKRNSLKGLTITWKNLNQADSIRW
                     GYTEAFEQGAFPGFRYNNYQLGDQPEYLFDYTFPGLKPRSTIYYSFKFNELWGKRMNF
                     TTACDTADTRFTFIVGADCQAGTEEFQQISSMAAAEKADFYINVGDIIDNSLYPGLWE
                     KYYFWGREYLEKTLTYYTHGNHLYYDTESGNAVMNQLVLPGNEKWYSFKQGNTLFICL
                     LSEASLSTQWTWLKNLLAKNTATWTVVYFHAPFFTSGGHAGEMDSAIPYWWKTFDDYG
                     VDVILNGHDHTYTRSKPINLNVSDSSAVAEYGSAPGQGRLQMLVGSFGAATYSEQSGW
                     WVGKTKSTVNYVKFGVDGDRMHFESIDLNGVIIDSLTLDARGTRYDRPINTGMERDAS
                     GLPVEFAVEQNYPNPFNPATKIAYSLPVTGKVALKIYNLLGREVLTLVDENKQAGRHE
                     VLFDASTLPSGLYYYRLQSGSFDQVKKMVFLK"
BASE COUNT          768 a         1145 c          861 g          730 t
ORIGIN      
        1 aaatagcaaa cctcctcccc agccctccct gcgcggtcag gtctgccagg gagggctata
       61 gaggtacaat catcacccct ccattccatt ctaaacatga ggctgctaat gaaaaaagtg
      121 tatctgtggc ttgcggggat tttgttaatc acagtttcat ctcttttcgc ccaaaccatt
      181 caccaggtgc ctgccggctc ggatgtcatc tctaccgcct atgaatcggc agcccccggc
      241 gatatcatcg agctgaccac cgacggcggc gagtacatcg aaggcaacga tctggtgatc
      301 atcaaggata aacccgtcac catccgcgcc gcatccgggt taaccaccaa gccccgctgg
      361 tccgcttccg gcggatgggc cctgctgcag acggccgacg acctcaccct cgaaggcatc
      421 tacgtcgacg gcacggtcgc cggcggcgcc cgtgaggtcg gcatccgcgg cgccgaaccc
      481 gagtggtatg ccctgcagct gggctatact ctcaagctcc tcaactgcga tttcgtcaat
      541 ttcgactacg ccgtctacgg cgaggattcc aatcaactgg acagccttat catcgatggc
      601 tgcacatttt cgcatatcat caagcaggcc atccagttca gcgacggcac catcgctccg
      661 ggccaggtca aacatttctt ctgcaccaat tccacctttt gggatatcgg cggctatgcc
      721 atctatctgc agtcgtccgc catcgacgcc aatccacaag ctgaatttgt cgtcgaccat
      781 gtgaccatcc atgatgcggc caatggtaat atctaccccc gcgatatcga tggggcggtc
      841 atcaaaaata cgatcataac caattcgggg ggctcgccgg gttcggtctg catcatcgcc
      901 ggcctgaact cccgcatcag caatctcctt tacaacggac tggaccaggt tagcctcgag
      961 gccggggcgg tggagagcca gctgaccagt gtctgggccg gggtcgatcc cctttacgcc
     1021 agcgctgtca gcggcaattt caccctcatg accggatcgc ccgccatcgg cgccggagat
     1081 gacggcaaaa gcctcggcga tccgcgctgg tggccggaga acaactccga gtacgtcttc
     1141 gtcttcgccg gcgaaaacac ccttgccgat gccgttgcgt cggctgtcgc cggccagacc
     1201 atcatgctgg tgtcggatgg cggtctctat gacaacaccc ataaaatggt gatcgacaag
     1261 gaactcacca tccgctccgc tgatggattg aaacagcggc cgctgctgac ctcgggcgct
     1321 tctgacgcca tcttcgagat cggcgccggc ttttccctcg aaggcgtcat cctcgatggc
     1381 gcgctcggcg ccgactccac tgccatcggc atcaccaatg ccccgaacac caccggctac
     1441 aacctcaagg tcctcaaaac cgatttcctc aacttttccg acaaaggtca gaccaccggc
     1501 ttcggcatct tcggcgtccc cagctcggtc gtcgataccg tcctcatcca ggactgctac
     1561 ttcgcccaca tcctcgatat gggcatcagc ttcaatgacc ccctcaccgc caccggctcg
     1621 gtcaatgtct tcaaggttga caactgcacc ttttgggaca tgaactccga agccatctac
     1681 atcgatgcct tcgattccaa aatggaaacc aaggatcccg aggtcaccat caacaaggtc
     1741 acggtctacg actgcggcag ctacaatctc atcgcccact atatcgatta ctccgtgctc
     1801 accaactcca tggtcgtcct ccccgaactc aacaccagct acgccccgac caaggtctac
     1861 ggcaccaact cgaaggtcga gaatttcctc tacttcaaca cccgcgacat cgacctcagc
     1921 tacggcgcca ccgatttcca gctcgtcaac atcgtcccgc agggtgaccc cggactcgtg
     1981 gacccggaga atggcgactt ttcctacacc gtccactccc cggccgtctt cttcctcgag
     2041 gatggtgatt tcctccgcct tggcgatgac cgctggtggc cgggtatcaa cctgcccgcc
     2101 aatatccatt ggggcaccaa acgtaactcc ctcaagggat tgaccatcac ctggaaaaat
     2161 ctcaatcagg ccgactccat ccgctggggc tacaccgagg cctttgagca gggcgccttt
     2221 ccgggttttc gctataacaa ttaccagctg ggcgatcagc ccgaatacct ctttgactat
     2281 acctttcccg gtctcaagcc gcgctccacg atttattatt cattcaaatt caatgaatta
     2341 tggggcaaac gcatgaactt caccaccgcc tgcgacacgg ccgacaccag gttcaccttc
     2401 atcgtcggcg ccgactgcca ggcgggcaca gaagaattcc agcaaatttc ctcgatggcc
     2461 gccgccgaaa aggccgattt ctacatcaac gtcggcgata tcatcgataa ctccctctat
     2521 cccggattgt gggaaaaata ctacttctgg gggagggaat atttggaaaa gaccctgacc
     2581 tattacaccc acggcaacca cctctactac gacaccgagt cgggaaatgc ggtgatgaac
     2641 cagctggtcc tgccgggcaa tgaaaaatgg tactcgttca agcaggggaa tacccttttc
     2701 atctgcctct tgtcggaggc ctccctctcg acgcagtgga cctggctcaa gaatctgctc
     2761 gccaagaata ccgccacctg gacggtggtc tatttccacg cccccttctt caccagcggc
     2821 ggacatgccg gcgagatgga ctcggcgatt ccctactggt ggaaaacctt tgacgattac
     2881 ggcgttgatg tcatcctcaa tggccacgat catacctata cccgatccaa gccgatcaac
     2941 ctgaacgtct ccgattcctc ggcggtcgca gaatacggca gcgcccccgg ccagggccgt
     3001 ctgcagatgc tggtcggcag tttcggcgcg gctacctact ccgaacaatc cggctggtgg
     3061 gtaggcaaaa ccaaatcgac agtcaactat gtcaagtttg gggtggacgg cgaccggatg
     3121 cacttcgaga gcatcgacct gaacggtgtc atcatcgaca gcctgaccct cgatgccagg
     3181 ggaacccgct atgatcgccc catcaacacc gggatggagc gcgatgcctc gggcttgccc
     3241 gtggagtttg ccgtagagca gaactatccc aatccgttca atccagcgac caaaatcgcc
     3301 tacagcctgc cggtcaccgg caaggttgct ctcaagattt acaacctgct cggccgtgag
     3361 gtgctgaccc tggtcgatga gaacaagcag gccggcaggc atgaggttct cttcgatgcc
     3421 tcaaccctgc ccagcggcct ctactattac cgcctgcagt cgggttcgtt tgaccaggtt
     3481 aaaaaaatgg ttttcctgaa ataa
//
LOCUS       DAOPWU010000350         3501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_27330_length_3501_cov_4.466338, whole genome shotgun sequence.
ACCESSION   DAOPWU010000350 DAOPWU010000000
VERSION     DAOPWU010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27330_length_3501_cov_4.466338"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1149)
                     /locus_tag="PKM23_13470"
     CDS             complement(130..1149)
                     /locus_tag="PKM23_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNY92527.1"
                     /translation="MQKIAALAGVSIATVSRALNKSGPVKEATRARILQLAGEYQYTP
                     NPIARSLSTQKTDTIGLILPEMADEFFMDIIRGIDEEAYRANRFLLVSSFHTQRDIAA
                     TLLEFMSSGRVDGVILMAPEGKNGLVETLRRSRRPLVLLNPPGNAQEFVYFKINNFQG
                     AYAIVEHLFGHGYHRIGMITGPNGNIDADERLNGCLEAGRQHDLPIAPSFIMPGDFSA
                     RSGYYAFMRLMSQMEKPEAVFAANDMMAMGAYQAARSLNLRIPQDIALAGFDDIFLSQ
                     LLTPRLTTVHVPIVELGSKAVRYLLQMINGEVDPSAPYHEELTAGLVIGGSCGCESVA
                     AAVPL"
     gene            complement(1251..2060)
                     /locus_tag="PKM23_13475"
     CDS             complement(1251..2060)
                     /locus_tag="PKM23_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY92528.1"
                     /translation="MARIGLHTLMLLGLAVTLLPFWWMVMASFMPGGEASTFPPRFWP
                     SRWSTEHYATLWERLQIGRFFLNSLILAGGVTLVSLLVNSLAGYAFAKFAFRGRKAIF
                     SLLLTSLVIPGQVTMLPVFLMLKQMGLLNSYFGIIIPGMASIFGIFLIRQYLQGVPDS
                     LIEAARIDGAGELRIYWTIILPLAKPILVTLALFTFMGTWNDFLWPLIVLNRQEMYTL
                     PVAIANLMGEHAPDPELMMAGAVVTILPILLLFLALQKYYIQGILMGGLKE"
     gene            complement(2156..3037)
                     /locus_tag="PKM23_13480"
     CDS             complement(2156..3037)
                     /locus_tag="PKM23_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY92529.1"
                     /translation="MKHKWLIPWFFLAPALGTLLLFFFLPVLASLLMSFSDFDIYALG
                     NLSRARWNGLENYGRLLHDPLFWKALGNTVYFVLAGGPLTILAALLAAIGLNSRQLML
                     RDWFRLGYFTPVITSLVAVAVVWRYLYHPRFGLFNYLLSFAGVGPVNWLGDPVWAMPA
                     LILLAVWKNFGYYMMIFLAGLQTIPDYLYEAARLDGAGWWGQFRHVTLPQLAPTTFFV
                     TLMTIIGYFQFFAEPYVMTQGGPVNSTLSVVFYLYQQGFRWWRMGYASSIAFMLFFII
                     FLLAMLQMAARRRQEAA"
     gene            complement(3048..>3501)
                     /locus_tag="PKM23_13485"
     CDS             complement(3048..>3501)
                     /locus_tag="PKM23_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY92530.1"
                     /translation="KRMPGLSGRWSTAPLPAGRNRASVAGGASLVLFEKCRHPEAAWK
                     FIRYLSEVETQQAFFSLTRDLPAVRAAWHTPEIENDRETGAFYRQLETAMPTPRIAEW
                     EQVAVKMQEHLEQVIYRRMSLEEAVVKLDRDVDRILEKRRWLLARDLL"
BASE COUNT          726 a         1003 c         1198 g          574 t
ORIGIN      
        1 tcccggcgcc ggcggccggg gaagaaaaac gcggcgggcc ggagaaccgg cccgtcacag
       61 gggggggaac cggcgccgat gaaagaaagt gcggtggcgc tttgtgctcc acctcccgcg
      121 tcgacaggct cagagcggta cggcggcagc caccgattcg cagccgcagg agcccccgat
      181 caccagtccc gccgtcagct cctcatgata gggcgccgac gggtcgacct cgccgttaat
      241 catctgcagc agatagcgga ccgccttgct gccgagttcg acgatgggca cgtgcacggt
      301 ggtcagacgc ggggtgagca gctggctgag aaaaatgtca tcgaagccgg ccagggcgat
      361 atcctggggg atacgcagat tgaggctgcg ggccgcctgg taggcgccca tggccatcat
      421 atcgttggcg gcaaagaccg cctccggctt ttccatctgg ctcatcaggc gcataaaggc
      481 gtaatacccg gaacgggcgc tgaaatcgcc gggcatgatg aaggaggggg cgatgggcag
      541 gtcatgctgc cgccccgcct ccaggcagcc gttcaggcgc tcgtcggcgt cgatgttgcc
      601 attggggccg gtgatcatgc cgatgcggtg atagccgtgg ccgaagaggt gttcgacgat
      661 ggcgtaggct ccctgaaagt tgttgatctt gaagtagacg aactcctgcg cattgccggg
      721 gggattgagc aggacgaggg ggcggcggct gcggcgcagg gtctcgacaa gcccgttctt
      781 cccctccggt gccattagga tgaccccgtc cacccggccg ctgctcataa attcgagcag
      841 ggtggcggcg atatcgcgct gggtatggaa gctggagacg agcaggaagc ggttcgcccg
      901 gtaggcctct tcatcgatgc cgcgaatgat gtccataaag aattcatcgg ccatctcggg
      961 caggatcagt ccgatggtat cggtcttttg tgtcgagagc gagcgggcga tgggattggg
     1021 ggtgtactgg tattcgccgg ccagctgaag gatgcgcgca cgggtcgcct ccttcacggg
     1081 accgcttttg ttgagggcgc gcgatacggt ggcgatggag accccggcca gcgcggcgat
     1141 tttttgcatg ttgaccacgg accgcctcaa gacttggcat gtaaagaagg gaaacgatta
     1201 cagtccataa atttactggt ttcggcatcc ttttgcaaga gaataatcgc ctactccttg
     1261 aggcccccca tgagaatccc ctggatgtag tatttctgca gggcgagaaa gagcagaagg
     1321 atcgggagaa tcgtcaccac cgccccggcc atcatcagct ccggatcggg ggcgtgctcg
     1381 cccataagat tggcgatcgc cactggcagg gtgtacatct cctggcggtt gaggacgatc
     1441 aggggccaga gaaaatcgtt ccaggtcccc ataaaagtga agagggccag ggtgaccaga
     1501 atcggtttgg ccagaggcag gatgatagtc cagtagatgc gcagctcccc ggccccgtcg
     1561 atgcgcgccg cctcgatcag gctgtcgggc accccctgga gatactggcg gatgagaaag
     1621 atgccgaaaa tgctcgccat gcccggaatg atgatgccga aatagctgtt gagcagtccc
     1681 atctgcttga gcatgagaaa gaccggcagc atggtcacct ggccgggaat gaccagcgag
     1741 gtgaggagca ggctaaagat cgccttgcgc ccccggaagg caaatttggc gaaggcatag
     1801 ccggccaggg agttgacgag caggctgacg agcgtcaccc ccccggcgag gatgaggctg
     1861 ttgaggaaga agcggccgat ctggagccgc tcccagaggg tggcatagtg ctccgtactc
     1921 cagcgtgaag gccagaagcg cggcgggaag gtgctcgcct cccccccggg catgaaagag
     1981 gccatcacca tccaccagaa gggcagcagg gtgaccgcca gaccgagcag catgagggtg
     2041 tgcaggccga tccgggccat caggcggccg tggcgccggc gaaaggcggt gagtcccccc
     2101 tcccccgtcg ctgcgccccg tttggtgcgc ggcgcgattc catcattccg ggcgctcatg
     2161 ccgcctcctg acgcctgcgc gcggccatct ggagcatggc gagcagaaag atgatgaaaa
     2221 agagcataaa agcgatcgag gaggcgtagc ccatccgcca ccagcggaag ccctgctggt
     2281 agagataaaa gaccacgctc agggtgctgt tgaccggtcc gccctgggtc atgacgtagg
     2341 gctcggcgaa gaactggaaa taaccgatga tggtcatcag ggtgacgaaa aaggtggtcg
     2401 gggccagctg cggcagggtg acatggcgga actgccccca ccagccggcg ccgtcgaggc
     2461 gcgctgcctc gtagagatag tccggaatgg tctgcagccc ggcgaggaag atcatcatgt
     2521 aatagccgaa atttttccag accgccagca ggatcagggc gggcatggcc cagaccggat
     2581 caccgagcca gttgaccgga ccaaccccgg cgaaggagag cagatagtta aagagcccga
     2641 agcggggatg gtagagatag cgccagacca ctgcgacggc gaccagcgaa gtgatcaccg
     2701 gggtgaaata gccaaggcgg aaccagtccc tcagcatgag ctggcggctg ttgaggccga
     2761 tggcggccag cagggcggca aggatggtga ggggcccgcc ggcgaggacg aaataaaccg
     2821 tattgccgag ggccttccag aagaggggat cgtgcagcag gcggccgtaa ttttcgaggc
     2881 cgttccagcg cgcccgcgag aggttgccca aagcgtagat gtcaaaatcg ctgaagctca
     2941 tcagcaggga ggccaggacg ggcaggaaga aaaagagcag gagcgtcccg agcgccggag
     3001 cgaggaaaaa ccagggtata agccacttgt gcttcatcca tcccccctca gagcagatca
     3061 cgcgcaagca gccagcgccg tttttcgagg atgcgatcga cgtcgcggtc cagtttgacg
     3121 acggcctcct ccaggctcat ccttctgtag atgacctgtt cgaggtgctc ctgcatcttg
     3181 accgccacct gctcccattc ggcgatccgg ggcgtgggca tggcggtctc cagctggcgg
     3241 taaaaggcgc cggtctcgcg atcgttttcg atctccgggg tgtgccaggc cgcccgcacg
     3301 gccggcagat cccgggtcag gctgaaaaag gcctgctgtg tctccacctc ggagagatag
     3361 cggatgaact tccaggctgc ttcggggtgg cggcattttt caaagaggac caggctggcg
     3421 ccccccgcca ccgaggcgcg gttgcggccg gcgggcagcg gggcggtgct ccagcgcccg
     3481 gaaaggccgg gcatccgttt g
//
LOCUS       DAOPWU010000351         3498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_27367_length_3498_cov_3.328493, whole genome shotgun sequence.
ACCESSION   DAOPWU010000351 DAOPWU010000000
VERSION     DAOPWU010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27367_length_3498_cov_3.328493"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PKM23_13490"
     CDS             <1..990
                     /locus_tag="PKM23_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNY92531.1"
                     /translation="PGVAGALHMDRMPIAGFIRTSSADHLVTDSAAASSAMACGIKTN
                     NGRISRTPNGDMPLSIMAAAKARGMATGLVATSSVTHATPAGFGAHTMSRKEQPLIAE
                     HLLLNGIDVMLGGGYSFFIPKSETGSRRADERNLLDLARTKNYAVIRTREELMAARGP
                     RVLGLFADEGMQTMRPEPTIAEMTARAIDLLKESKKGFIVMIEGSQIDWANHDHNAEN
                     SVRQTLDFDMAVAEALKFAQKDKHTLVVVTADHETGGLSINGGNVDGSEVELHWSTDG
                     HSGATVPLYAYGPGAKLLSGFHENTDIPKAFSRLLKIENFPAIIEKAPAEPGQ"
     gene            complement(1047..1520)
                     /locus_tag="PKM23_13495"
     CDS             complement(1047..1520)
                     /locus_tag="PKM23_13495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNY92532.1"
                     /translation="MKFLSIADDVKLGEGVKIFNFVNLYGCEIGDHTKIGTFVEIQKG
                     ATVGKHCKISSHTFICEGVHIEDNCFIGHNVTFINDKFPRATNPDGSLQTEADWACVP
                     TYVRTGASVGSSSTILCGVTIGENAIIGAGAVVTKDVPANTIVAGCPARVLRKME"
     assembly_gap    1585..1684
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1685..2588)
                     /locus_tag="PKM23_13500"
     CDS             complement(<1685..2588)
                     /locus_tag="PKM23_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY92533.1"
                     /translation="MINVGVIGVGYWGPNIIRNFDGCSQTQMAIASDLKPERLSFIQK
                     KHPALKTTADYREIINDPAIDAVAICTPVFSHYELAKEALQAGKHVFLEKPMTSTSQQ
                     AEELVNLAAQKKLTLMLDHTFIYTGAVQLIKKLVDAGEIGDLYYFDSVRINLGLFQHD
                     VNVIWDLAPHDISIMDHLLQLKPESVVATGADHVGAGHEDVAYLTVYFPNNIIAHIHA
                     NWLSPVKIRQTLIAGTKKMIVWDDNLPNDKIKIYDSGITVNRTKAEIYDTLIQYRTGD
                     MWAPKVPLTEALSLEVNHFAECIQS"
     gene            complement(2689..>3498)
                     /locus_tag="PKM23_13505"
     CDS             complement(2689..>3498)
                     /locus_tag="PKM23_13505"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNY92534.1"
                     /translation="GGQALDPHLQVQPMRRLCIGIGEAMLYLSSAPEFNYLNPLLAWY
                     GEVTNGPVGGNILGSVHIAAMPVDNLTLHADLLIDDIQLEKSGPGDLEPAEYGLIAGL
                     RWADPLHLQGGDLFLEYTRITNRTFNGEGGPWERWLHRNEPIAHFLGNDFERWLAGFS
                     WWPQPALRAACIIDLRRRGEGRIDKPFDRPWLDTPLGEEYSEPFPTGVVEENERCTLE
                     LSWQPRQAVRAHGRIGYCRISNLNHSPGLDRSEWMGSLGLEVDVLRKFSME"
BASE COUNT          658 a         1029 c         1046 g          665 t
ORIGIN      
        1 ccgggggtcg ccggcgcact ccatatggac cgtatgccca tcgccggctt tatccgcacg
       61 agctcggcgg accacctcgt caccgattct gcagccgcca gcagcgccat ggcctgcggc
      121 atcaaaacca acaacggccg catctcccgc acaccgaacg gggatatgcc cctgagcatc
      181 atggccgccg ccaaagccag agggatggcc accgggctgg tcgctacctc ctcggtcacc
      241 cacgccacac cggccggctt cggcgcccac accatgagcc gcaaggagca gcccctcatt
      301 gctgagcacc tgcttctcaa cgggatcgat gtcatgctgg gcgggggata ctccttcttc
      361 atcccgaaga gcgaaaccgg cagccgccgg gcggacgagc gcaacctcct cgacctcgcc
      421 cgcaccaaaa attatgcggt catccgcacc cgcgaggaac tgatggccgc cagggggccg
      481 agggtgctcg gcctcttcgc cgatgagggg atgcagacca tgcgtcccga gccgaccatc
      541 gccgagatga ctgcgcgggc catcgatttg ctcaaggaga gcaaaaaggg ctttatcgtt
      601 atgatcgagg gcagtcagat cgactgggcg aaccacgacc acaacgcgga gaactcggtc
      661 cgccagaccc tcgactttga catggcggtc gcggaagccc tcaaattcgc gcaaaaggac
      721 aaacacaccc tggtcgtggt caccgccgac catgagaccg gcggcctctc catcaacggc
      781 ggcaatgtcg acggcagcga ggtcgaactc cactggagca ccgacggcca ctccggcgcc
      841 accgtccccc tctacgccta tggtcccggg gcgaagctgc tctccggatt ccatgaaaac
      901 accgacatcc ccaaggcttt ctcccgcttg ctcaagatcg aaaactttcc ggcaatcatc
      961 gaaaaagccc ccgccgagcc cggccagtag agaattaaaa aagggagatg gatttatttc
     1021 catctccctt tttaatatct gcccggctat tccatcttcc tcagcaccct cgccgggcag
     1081 ccggcgacga tggtgttggc cggcacatct ttggtcacca ccgctccggc gccgatgatg
     1141 gcgttctcgc cgatggtcac cccgcagagg atagtcgagc tcgagccgac cgaagccccc
     1201 gtacggacgt aggtgggcac gcaggcccag tccgcctcgg tctgcaggga gccgtccgga
     1261 ttggtcgcgc gcgggaattt gtcgttgatg aaggtgacgt tgtggccgat gaagcagttg
     1321 tcctcgatgt gcaccccctc gcagatgaag gtatggctcg agatcttgca gtgcttgccc
     1381 accgtggccc ctttctggat ctcgacgaag gtgccgatct tggtatggtc gccgatctcg
     1441 cagccgtaga ggttgacgaa attgaagatc ttgacccctt cgccgagttt gacatcgtcg
     1501 gcaatgctga gaaatttcat gttctgctcc aggagtaggt tattttatac aggttcacaa
     1561 aaaacagtgt caggttttac gccannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnncgctct ggatgcattc ggcgaaatgg ttcacctcca ggctcagcgc ttcagtgagc
     1741 ggcaccttgg gggcccacat gtcgccagtg cggtactgga tcagggtgtc atagatctcc
     1801 gccttggtgc ggttgacggt gatgcccgaa tcgtagatct tgatcttgtc gttggggaga
     1861 ttgtcatccc agacgatcat ttttttggtc cccgcgatca gggtctggcg gatcttgacc
     1921 ggcgagagcc agttggcgtg gatgtgggcg atgatgttgt tggggaaata gacggtgaga
     1981 taggcgacat cctcgtggcc tgcgccgacg tggtcggcgc cggtggcgac cacactctcg
     2041 ggtttgagct ggaggaggtg atccatgatc gaaatgtcgt gcggggccag atcccagatc
     2101 acattgacgt cgtgctgaaa gaggccgagg ttgatgcgca ccgaatcaaa ataataaaga
     2161 tccccgatct cgccggcatc gaccagtttt ttaatcagct gcaccgcgcc ggtgtaaatg
     2221 aaggtatgat cgagcatcag ggtgagcttt ttctgcgccg ccaggttgac cagttcctcg
     2281 gcctgctgcg aggtggaggt catcggcttt tcgagaaaga cgtgcttgcc ggcctgcagg
     2341 gcctctttgg ccagctcata gtgggaaaag accggggtgc agatcgccac cgcatcgatg
     2401 gcgggatcgt tgatgatctc gcgataatcg gccgtggttt taagagcagg gtgcttcttc
     2461 tggatgaagg agagccgctc cggcttgagg tccgaggcga tggccatttg ggtctgactg
     2521 cagccatcga aattgcggat gatattgggt ccccagtagc cgacgccgat gacaccgaca
     2581 ttgatcataa cacctccgat gagtaatgat gctttttatt gatctgccgg gattggcccg
     2641 gccactgtaa cactctcccc ctgcttcccc tgatggctcg aagcaaaact attccatgct
     2701 gaatttgcgc agcacatcca cctccagccc caggctgccc atccactcgc tgcggtccag
     2761 ccccggggaa tggttcaggt tggaaatccg gcaatagccg atacggccgt gggcgcgcac
     2821 ggcctggcgc ggctgccagc tcagctccag agtgcagcgt tcgttctctt cgaccacgcc
     2881 ggtgggaaag ggctcgctgt actcctcccc gaggggggta tcgagccagg gacggtcgaa
     2941 gggcttgtcg atgcgcccct cgccgcgccg gcgcagatcg atgatgcagg cggcgcgcag
     3001 ggccggctgc ggccaccagg agaaaccggc cagccagcgt tcgaaatcgt tgccgaggaa
     3061 atgggcgatg ggctcgttgc gatgcagcca ccgctcccag ggcccgccct cgccgttgaa
     3121 ggtgcgattg gtgatgcgcg tgtactcgag aaagaggtcg ccgccctgaa ggtgcagggg
     3181 atcggcccag cgcaatccgg cgatcaggcc gtattcggcg ggttccaggt ccccgggacc
     3241 gctcttctcg agctggatgt cgtcaatgag gaggtccgca tggagggtca ggttgtccac
     3301 cggcatggcg gcgatgtgta cgctgccaag gatgttgccg ccgaccggcc cgttggtgac
     3361 ctcgccgtac caggccagaa ggggattgag atagttgaat tccggggcgc tgctcaagta
     3421 gagcatcgcc tcgccgatgc cgatgcagag ccgccgcatg ggctgcacct ggaggtgggg
     3481 gtccagagcc tgtccccc
//
LOCUS       DAOPWU010000352         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_27621_length_3475_cov_4.216082, whole genome shotgun sequence.
ACCESSION   DAOPWU010000352 DAOPWU010000000
VERSION     DAOPWU010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27621_length_3475_cov_4.216082"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..2284)
                     /gene="fusA"
                     /locus_tag="PKM23_13510"
     CDS             complement(200..2284)
                     /gene="fusA"
                     /locus_tag="PKM23_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNY92535.1"
                     /translation="MSTPPSLDKTRNIGIMAHIDAGKTTTTERILYYTGKVHRMGEVD
                     DGAATMDWMEQERERGITITSAAITCSWKNHRINIIDTPGHVDFTAEVERSLRVLDGA
                     VAIFDAAGGVEPQSETVWRQADKYHIPRIAYVNKMDRIGADFGMVLDSMRERLHTRPV
                     PIQLPLGAEDRFTGLIDLITMKAVHYDEATLGVTWEEREIPAALDEQARSMRRELIEA
                     VADFDDEVMEKYLGNEEITSAEIQRALRAGTLKDAIVPVLCGASVKNRGVQHLLDAVV
                     SYLPSPLDIPPVEGVNPYTERIEKRSADPDEPFTALAFKIMTDPFVGKLTYFRVYSGR
                     IAAGETALNSGTEKKERLSRILLMSSNKRDDVQGVAAGDIAAAVGLRNTRTGDTLCDP
                     KHPLMLESMNFPIPVISIAIEPRSKADQERMSAALTALADEDPTFQYSVDAETGQMII
                     SGMGELHLEILVDRLLREFKVQANIGRPQVAYRETITEPVTSEIEFVKQTGGKGQYAH
                     VVVEFEPGEQGKIFSFTNLASPLEVPNQFVRPVEQGIKEAMVSGVIAGYPIIDIKATL
                     TGGSFHDVDSTELAFKIAGSMALQDAARRGKAILMEPVMELEVVTPEEYFGSILSDLA
                     ARRARIANHHKRRDAQVISAQAPLAEMFGYATALRNTSQGRAIFTMQFSSYQKVTEEV
                     GKKLLQKMGLMV"
     gene            complement(2513..2983)
                     /gene="rpsG"
                     /locus_tag="PKM23_13515"
     CDS             complement(2513..2983)
                     /gene="rpsG"
                     /locus_tag="PKM23_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNY92536.1"
                     /translation="MSRRKSPPKRTVLPDPKFNSVLVTKFINGLLRGGKRSVAETIFY
                     DAIDIIAAKTSADGLPVFEKAMHNVKPMLEVRSRRVGGATYQVPVDIRGDRQQALAIR
                     WIIGYAKNRSEKTMSEKLAFELMAAAKNEGNSIKKREDTHKMAEANRAFAHFRW"
     gene            complement(3023..3394)
                     /gene="rpsL"
                     /locus_tag="PKM23_13520"
     CDS             complement(3023..3394)
                     /gene="rpsL"
                     /locus_tag="PKM23_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNY92537.1"
                     /translation="MPTINQLVRTGRESLTKREKAPALNKSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLSNQIEVTAYIPGEGHNLQEHSIVMIRGGRVKDLPGVRYHIVRGVL
                     DTSGVQDRKQGRSKYGAKRGK"
BASE COUNT          619 a          990 c         1083 g          783 t
ORIGIN      
        1 atcgtgccga tgttcacatg cggcttggtc cgctcgaact tttgctttgc catttctgtt
       61 tctccttgac ttcgctattt tttaaaacca tttctcacag aggcacggag acacaaagaa
      121 aaacgtgctc acagaattct aatcgcctta tctcgtttct atctctctct gtggctctgt
      181 gccaccgtga gagcgtcttt tataccatca gccccatttt ttgcaggagc tttttgccca
      241 cctcttcggt gaccttctgg tagctggaaa actgcatcgt aaagatcgcc ctgccctggg
      301 aggtgttgcg cagggcggtg gcgtagccga acatctcggc cagaggcgcc tgggcgctga
      361 tcacctgggc atcgcggcgc ttgtgatggt tggcgatccg ggcccgccgc gcggccagat
      421 cgctcaggat cgaaccaaag tactcttccg gggtgacgac ctcgagctcc atcaccggct
      481 ccatcaggat cgccttgccg cgccgggcgg cgtcctggag ggccatcgaa ccggcgatct
      541 tgaaagccag ttcggtcgag tcgacatcgt gaaaggaacc gcccgtcagg gtcgccttga
      601 tgtcgatgat cggataaccg gcaatgaccc cgctcaccat cgcctccttg atcccctgtt
      661 ccaccggccg cacgaactgg ttcggcacct ccagagggct ggcgagattg gtgaaagaaa
      721 agatcttgcc ctgctcgccg ggctcgaact cgacgaccac atgcgcgtac tgccccttgc
      781 cgccggtctg cttgacgaac tcgatctcgc tggtcaccgg ctcggtgatg gtctcgcgat
      841 aggcgacctg cggcctgccg atattggcct ggaccttgaa ctcgcgcagc agccggtcga
      901 ccaggatctc gaggtgaagc tcgcccatcc ccgaaatgat catctgcccg gtctcggcgt
      961 cgacgctgta ttgaaaggtc ggatcctcgt cggccagggc ggtcagggcg gcgctcatgc
     1021 gctcctgatc ggccttggac ctgggctcga tggcgatcga gatcaccggg atcggaaaat
     1081 tcatgctctc cagcatcagg ggatgcttgg ggtcgcagag ggtgtcgccg gtgcgggtgt
     1141 tgcgcagccc gaccgcggcg gcgatatcgc ccgcggcgac gccctggacg tcgtcgcgct
     1201 tgttggacga catcagcagg atcctgctca gcctctcctt cttctcggtg ccgctgttga
     1261 gggcggtctc gcccgccgcg atgcggcccg agtagacccg gaagtaggtg agcttgccga
     1321 cgaaggggtc ggtcatgatc ttgaaggcca gcgccgtgaa gggctcgtcg ggatccgcgg
     1381 agcgcttttc gatccgttcg gtataggggt tgaccccttc gaccggcggg atatcgagcg
     1441 ggctgggcag gtagctgacc actgcatcga gcaggtgctg gacgccgcgg ttcttgaccg
     1501 aggccccgca caggaccggg acgatggcat ccttgagggt gccggcgcgc agggcgcgct
     1561 ggatctcggc ggaggtgatc tcctcgttgc cgaggtactt ctccatgacc tcgtcgtcaa
     1621 agtcggcgac ggcctcgatc agttcgcggc gcatcgagcg cgcctgttca tcgagggcgg
     1681 cgggaatctc gcgctcctcc caggtgactc cgagggtggc ttcgtcgtaa tggaccgcct
     1741 tcatcgtgat cagatcgatg aggccggtga agcgatcctc ggcgccgaga ggaagctgga
     1801 tcggaacggg ccgggtgtga agccgttcgc gcatcgaatc caggaccatg ccaaagtcgg
     1861 cgccgatgcg gtccatcttg ttgacgtagg cgatgcgggg gatgtggtat ttgtccgcct
     1921 gccgccagac cgtctccgac tggggctcga cgccgccggc ggcatcgaag atggcgactg
     1981 cgccatcgag tactctcaaa gaacgttcga cttcggccgt gaaatcgacg tggccgggtg
     2041 tgtcgatgat attgatacgg tggttcttcc agctgcaggt gatggcggcc gaggtgatgg
     2101 tgattcctct ttcgcgttcc tgctccatcc agtccatggt ggcggcgccg tcgtcaacct
     2161 cgcccatccg gtgcaccttg ccggtgtagt agagaatccg ctcggtggtg gtggtcttac
     2221 cggcatcgat atgagccatg atgccgatat tgcgggtctt gtccagagag ggaggggttg
     2281 acataaaaaa gccacttacc ttaaaagagg cgcctcctag attaagattc ggagcgcggg
     2341 tgcgtgggca tcagaaagtt ggcgcgccac gcatcctctt cacggtcaag tggtatcgaa
     2401 gagtgagctg tttgtgcgac gccaagttct ggcacccact ttccgggccg gctatcttta
     2461 agccttgctg gtccggagtt agaatgcaga actggccaaa accagaacct gtttaccagc
     2521 ggaaatgggc gaaggccctg ttggcctcgg ccattttatg ggtatcttcg cgtttcttga
     2581 tcgaattgcc ttcgttcttg gccgcggcca tcagctcgaa ggcgagcttt tcggacatgg
     2641 tcttttcgga acgattcttg gcatagccga tgatccagcg gatggccagg gcctgctggc
     2701 gatccccgcg gatgtcgacc ggcacctgat aggtggcgcc gccgacgcgg cgggagcgca
     2761 cttcgagcat cggcttgacg ttgtgcatcg ccttttcaaa gaccggcagc ccatccgcgc
     2821 tagtcttggc ggcgataata tcgatggcat cgtaaaaaat ggtctccgcc acgctgcgtt
     2881 tgccgccgcg gagcaggcca ttgatgaact tggtgaccag tacgctgttg aatttgggat
     2941 caggcagtac ggtacgtttc ggcggtgatt ttcttcttga catgaaaggc tctcaaaata
     3001 atgggatgat cgggacggcg gcttacttgc cgcgcttggc gccgtatttg gaacggccct
     3061 gcttgcggtc ctgaacacca ctggtatcca aaacgccgcg gacgatatga tagcggacgc
     3121 ccggcaaatc cttgacgcgg ccgccgcgga tcatgacaat cgaatgctcc tggagattat
     3181 ggccctctcc gggtatatag gcagtgacct cgatctggtt gctgaggcgc acgcgcgcca
     3241 ccttgcgcaa cgcagagttg ggcttcttgg gagtcgtggt atagacgcgg gtgcagacgc
     3301 cgcgacgttg cggtgatttg ttgagcgccg gcgctttttc acgtttggtc agggactcgc
     3361 ggccggtgcg caccaattga ttgatggttg gcactctacc tccagttttt ggctaagcct
     3421 tttaatataa ggtattattt gataaatttc aagaaattta tttaaatatc gtacg
//
LOCUS       DAOPWU010000353         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_27868_length_3451_cov_3.516490, whole genome shotgun sequence.
ACCESSION   DAOPWU010000353 DAOPWU010000000
VERSION     DAOPWU010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27868_length_3451_cov_3.516490"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PKM23_13525"
     CDS             complement(<1..886)
                     /locus_tag="PKM23_13525"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNY92538.1"
                     /translation="MRRLGRFWKPVMVTLVFLLGFAAFLHSGMVPGDTPERVLRALYY
                     ALSLFFLGGLDIGLPGTPSSPVTILLWLLYFLAPVLTAGYVYDFIQKKLLGRIPRGLK
                     GHTILCGLGRNGHLIYELIRETLPRGHKLVVIEKSETNPYLAAVRKDPHAWLIQNDFS
                     RLPVLESARIKRARRVIFSTNLDLDNLNALMELQSHEPAVPGQSVHCHLGDLEMLDNL
                     QQTLFREEVYKRVHVFNGYQCATRQLYQMLTTGPFLGKEGTVFILFGYGRFAHMLFSH
                     ICSDPSRSGADEIIIVTQK"
     gene            complement(900..>3451)
                     /locus_tag="PKM23_13530"
     CDS             complement(900..>3451)
                     /locus_tag="PKM23_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HNY92539.1"
                     /translation="SMVKAKPLAVVAGDALVDWFEASSVRKPQTESGSDALHWASQPR
                     IERWVQPGGSLLVADFLRAAGTRAVASPVARGLRQIPCSRIIHSYADVELFPLGSGGK
                     ERDEKSYRVQAFNGYCGPSRGKPAQLPIRGDKAVAELVVLDDNNNGFRQRPEVWPLAV
                     TRKKEAVVILKTCSPVAQGPLWDHLSAHFADRLITVVKADDLRLEGVHISRRLSWERT
                     VKDLMWQYQNHPSFSALRQSAFLIVRFGVEGAVLLCNREPECKGYLYYDPGIGEESFR
                     QLYKGAMPGMGSAFTAGLAICVSEAREEEIRRGIQHGLLCAREVWKKGFGPDARRFAY
                     ARDAFAPGPLRKTAIEEVRIPDFLCEEGSRSESWSLLHDLATRGLEQAACFYVEHGWD
                     TALDRVPAARYRDLYSIDRSEIESYRSIHNLIHEYLNSEKKSKPLAVAVFGAPGSGKS
                     FGIGEIAESVAPGRIEKIEFNLSQFTAVQDLIAAFHKIRDIALGGRIPLVFFDEFDSE
                     FGGANGWLRFFLSPIQDGSFREGEAIHPLGKAIFVFAGGTHPTFAEFAAGGKKAGSDN
                     CAAFSAAEKAAKLPDFISRLRGFINIKGPNPAGAEDPYYIVRRALLWRFLISSNAKHL
                     LGSDKKLAINRSVLRALLRVPCYKHGVRSMIALLDMSMLANRRSFEPAALPSRTQLNL
                     HVDADAFYDLVLQDTLLSSQIEKMAKAIHERYRMDQAGRKSASDPAMQPWENLKSDLI
                     RSNLDAAADIPIKLEALGYTFVPERGDIKPIEFDPVEVERLALMEHERWNRERRTAGW
                     TLGERNADAHTTPYLVPWEQLPEDVKEWDREAVRAIPRALADAGFRVEKIK"
BASE COUNT          662 a          988 c         1120 g          681 t
ORIGIN      
        1 ttttttgggt gacgatgatg atttcgtcgg ccccgcttct gctgggatcg ctgcagatat
       61 gggagaagag catgtgggcg aagcggccgt aaccgaagag gatgaagacc gtcccctcct
      121 tgccaagaaa agggccggtc gtcagcatct ggtagagctg ccgggtggcg cactggtaac
      181 cgttgaagac atggaccctt ttgtagacct cctcccggaa gagggtctgc tgcagattgt
      241 cgagcatctc cagatcgccg agatggcagt gcacggattg gccggggact gccggctcat
      301 gcgactgcag ctccatcaga gcattgagat tgtccagatc caggttggtg gaaaaaataa
      361 cccgtcgggc tcttttgata cgcgcagatt ccagcaccgg caggcgcgaa aagtcattct
      421 ggatgagcca ggcatgggga tccttgcgga cggctgcgag gtagggattg gtctcgcttt
      481 tttcaatgac gaccagcttg tgtcccctgg ggagggtctc cctgatcagt tcataaatca
      541 gatggccgtt gcgcccgagt ccgcaaagga tggtgtgccc cttgagtccg cgggggatgc
      601 ggccgaggag ttttttctgg ataaaatcat agacatagcc ggccgtgagc acgggtgcaa
      661 gaaagtagag aagccagagc aggatcgtga caggggagga gggagtaccg ggcagaccga
      721 tatccaggcc gccgagaaaa aagagtgaga gggcatagta aagagccctg agcacgcgtt
      781 cgggggtatc tcccgggacc atgccgctgt gaagaaaggc agcgaagccg aggagaaaga
      841 ccagggtgac catgactggc ttccagaaac gtcccagacg gcgcatcgca ctctcccggt
      901 tattttatct tttcgaccct gaatccggca tcagccaggg ctctggggat ggcccgcacg
      961 gcctcgcggt cccactcctt gacatcctcg ggcagctgct cccagggcac caggtagggt
     1021 gtggtatggg cgtcggcatt gcgctcgccc agggtccatc cggcagtgcg gcgctcccgg
     1081 ttccaacgct catgttccat gagggcgagg cgttcaacct ccaccgggtc gaattcgatg
     1141 ggcttgatat cgccgcgctc cggcacaaag gtataaccga gcgcctccaa cttgatcggg
     1201 atatctgcag ccgcatcgag attggatctg atcaggtcgg atttgagatt ttcccagggc
     1261 tgcatggccg gatccgaggc ggatttgcgt cccgcctgat ccatccgata tcgttcgtgg
     1321 atggccttgg ccattttttc aatctggctg ctcagcaggg tatcctgcag gacgagatcg
     1381 taaaaggcat cagcatcgac atgcaggttc agctgcgtcc tggagggcag ggcggctggc
     1441 tcgaaggacc tgcggttggc cagcatgctc atgtcgagga gggcgatcat ggagcggacg
     1501 ccgtgtttgt agcagggcac ccgcagcagg gcgcgcagca cactgcggtt gatggcgagc
     1561 tttttgtcgg atccgagcag gtgtttggcg ttgctgctga tgaggaatcg ccagagcagc
     1621 gcccggcgga cgatgtaata gggatcttcc gctccggccg gattggggcc tttgatgttg
     1681 atgaaaccgc gcagccggct gatgaaatcg ggcagcttgg cggccttttc cgccgcgcta
     1741 aaggccgcac agttgtcgct cccggccttt ttgccccctg cggcaaattc ggcgaaggtg
     1801 ggatgggtgc cgccggcgaa gacgaagatg gctttgccga ggggatgaat ggcctcgccc
     1861 tcacgaaagg agccgtcctg gatggggctg aggaagaacc tgagccagcc gttggcgccc
     1921 ccgaattcgg agtcgaattc gtcgaagaaa accagaggga tgcggccgcc cagggcgata
     1981 tccctgattt tgtgaaaggc ggcgatcaga tcctgcaccg cggtgaattg ggagagattg
     2041 aactcgatct tttcaatccg gccgggtgcg acgctctcgg cgatctcgcc gatgccgaag
     2101 gacttgccgg agccgggcgc gccgaagact gccacagcga ggggtttgct cttcttttcc
     2161 gaattcaggt attcatggat gagattgtga atgctgcgat agctctcgat ctcggagcgg
     2221 tcgatgctgt aaaggtcccg gtatcgcgcc gccggcaccc ggtcgagcgc ggtgtcccag
     2281 ccatgttcca cataaaaaca ggccgcctgt tcgagcccgc gcgtggccag atcgtggagc
     2341 agcgaccagg attcggaccg cgaaccttcc tcgcagagaa aatcggggat ccggacctct
     2401 tcaatggccg tctttctgag cggaccggga gcgaaggcgt cgcgggcata ggcgaagcgg
     2461 cgcgcatcgg ggccgaagcc ctttttccaa acctcgcgtg cgcacaagag tccgtgctga
     2521 attccccggc ggatctcctc ctccctggcc tcactgacgc agatggccag gccggcggtg
     2581 aaggcgctgc ccatgcccgg catcgccccc ttgtagagct ggcggaaact ctcctcgccg
     2641 atgcctggat cgtaatacag gtagcccttg cactctggct cccggttaca gaggagcaca
     2701 gcgccctcca caccgaagcg gacgatcaga aaggcgcttt gcctgagggc actgaaagag
     2761 gggtgattct ggtactgcca catcagatcc ttgactgtcc gctcccagga gagcctccgt
     2821 gagatatgga ctccctcgag tctcagatca tccgccttga cgacggtgat cagcctgtcg
     2881 gcgaaatggg cgctgagatg atcccagagc gggccctggg cgaccggcga gcaggtcttg
     2941 aggatgacga ccgcctcttt cttcctggta acggcgagcg gccaaacctc gggccgttgc
     3001 ctgaagccgt tgttgttgtc atccaggacg accagctcag cgacggcctt gtcccccctg
     3061 atgggcagtt gggccggttt accccggctg gggccgcagt agccgttgaa ggcctggaca
     3121 cggtagcttt tttcatcgcg ctccttgccg ccgctgccga gcggaaacag ttcgacgtcg
     3181 gcgtaggagt ggataatcct gctgcagggg atctgcctca ggccgcgcgc caccggactg
     3241 gcgacggcgc gagtgcccgc cgcgcgcaga aagtcggcca ccagcaggga tccacccggc
     3301 tgcacccacc gctcgattcg gggctgactt gcccagtgca gcgcgtcgga tccgctttcg
     3361 gtctggggtt ttctgacact gctggcttca aaccagtcga ccagggcatc gccggcgact
     3421 acggccagcg gttttgcttt caccatagac t
//
LOCUS       DAOPWU010000354         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_27904_length_3448_cov_5.090186, whole genome shotgun sequence.
ACCESSION   DAOPWU010000354 DAOPWU010000000
VERSION     DAOPWU010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27904_length_3448_cov_5.090186"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PKM23_13535"
     CDS             <1..575
                     /locus_tag="PKM23_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY92540.1"
                     /translation="PISPSTYMFAFRRWYEEQNIFVLLNMGSRAENASVNLPLTSLNL
                     DSTKTWYLTELLSGEVLSVKPGSLENLNVAMDKYNARIYLLADTVTVTGIRNAPHAAR
                     PESMALQQNYPNPFNPSTTIRFTLTWSGHVRLQIYNIKGEIVATLVDEQRKAGEYEVP
                     FNGAHLASGLYFCRLESAGLTAVRKMMVTK"
     gene            601..1629
                     /locus_tag="PKM23_13540"
     CDS             601..1629
                     /locus_tag="PKM23_13540"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNY92541.1"
                     /translation="MKILVIGGSGFLSSVVVAILLERGHQVTTFTRGRSGERPATTAN
                     LIRLTGDRHDPAALQNALLAGPFDAVYDFVAYEPAESRLAAKTFHGRVGRFIHCSTIS
                     VYLVSDQVICPITEEQDKAPLMPFWERNPFGMTYGINKRACEEILWQAHDERQFPVSM
                     LRPTFISGPHDPAKRDFFWIERINDGGPLLVPGHGEFLFQSVYVEDAARAFTDLLEAE
                     NSIGKAYNVAAEEILSLNDYLHLLARLMGRDIRFVHLDQELFDRLPFSRHPGGDVFPF
                     NTRRDATFSLEEISRDLGYHTTPIAQWLATTIDWFTTHYNGHSTGYEARAQELAVIQK
                     IDEDRSPL"
     gene            1812..2318
                     /locus_tag="PKM23_13545"
     CDS             1812..2318
                     /locus_tag="PKM23_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNY92542.1"
                     /translation="MGKVLVLFDSETGNTRRMAGYVAEGARSIEGTEVRLLSVDDASR
                     EDVLWCDGIAVGSPTNMGVLSWKMKRFWDREMWDLWSKIDGRIGCAFASSGGWGGGAE
                     ITCQSILTVLINFGYLVFGAPDYVADRFTLHYGAVAAGEPRHEREIAACRKLGERLAY
                     WVALYSKK"
     gene            2330..3214
                     /locus_tag="PKM23_13550"
     CDS             2330..3214
                     /locus_tag="PKM23_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNY92543.1"
                     /translation="MREKILLDTDIGMDVDDAVCLAYLLAHPECDLLGITTVTGEAVE
                     RAKLASVICRAAGREIPIFPGAEDPLIVEQKQKCAPQAAALVRWDHQTNFPKGEAVEF
                     MRKTIRAHPGEVILLAIGPLTNLGLLFAVDPEIPALLKGVVMMGGYFFHRLPEVSPVE
                     WNASGDPHASAIVYRARVPLHRSVGLDVTRQVILTAEEVRRRFKSELLRPVLDFAEIW
                     FAGYYPSITFHDPLAAATIFDERLCTFRQGSITIELEDAANAGYTHWRDGGEENPHEV
                     AESVDAEAYFRHFFSICG"
     gene            3261..>3448
                     /locus_tag="PKM23_13555"
     CDS             3261..>3448
                     /locus_tag="PKM23_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92544.1"
                     /translation="MLKHRIMLTAAIVALTLLVSCIDRENKQGRVTLTFWHSFVSSTL
                     PALDDLISRFESEYPAIH"
BASE COUNT          708 a         1064 c          957 g          719 t
ORIGIN      
        1 tgcccatctc gccatccact tacatgtttg ctttcagacg ctggtatgag gagcagaaca
       61 tctttgtgct tctcaatatg ggcagccgcg ccgaaaacgc atcggtgaac ctgccgctca
      121 ccagcctgaa cctcgattcc accaaaacct ggtatctcac cgaactcctc agtggcgagg
      181 tcctctccgt caaaccaggc agcctggaga acctcaacgt ggccatggat aaatacaatg
      241 ccagaattta tctcctggcg gacaccgtga cggtgaccgg catccggaac gccccgcacg
      301 ctgcccggcc ggaatccatg gcactgcagc agaactatcc caatcccttc aatccctcca
      361 ctaccatccg cttcacactc acctggagcg gtcatgtccg gctgcagatc tataatatca
      421 agggtgagat cgtcgccacc ctcgtcgatg aacagcgcaa agcgggtgag tatgaagtgc
      481 cctttaatgg cgcccatctc gccagcggcc tctacttctg ccggctcgag tcggccggac
      541 tcacagcggt gcgcaagatg atggtgacca aataggcccc aggcggagag ggaaaccagg
      601 atgaaaatcc tcgtcatcgg cggcagcgga ttccttagca gcgtcgtagt agcgatcctg
      661 cttgaaagag gccatcaggt cactactttc actcgtggac gctccgggga aaggcccgcc
      721 acaaccgcga acctcatccg cctgaccggg gatcgccatg atccggccgc attgcaaaat
      781 gccctgctcg ccggcccctt cgatgcggtc tatgattttg tcgcctatga gccggctgaa
      841 tcccggctcg ctgccaaaac ctttcacggc agggtcggcc gctttatcca ctgcagcacg
      901 atctcggtct atctggtctc agaccaggtc atctgcccga tcacagagga acaggacaag
      961 gcccccctga tgcccttttg ggaacgcaat cccttcggca tgacctatgg catcaataaa
     1021 cgagcctgtg aggagatcct ttggcaggcc catgatgaac gacaatttcc cgtcagcatg
     1081 cttcgcccca ctttcatttc cggtccgcac gatcccgcca aacgggactt tttctggatc
     1141 gaacgcatta acgacggcgg gcctctgctc gtgcccggcc atggtgaatt cctctttcag
     1201 tcggtctatg tggaggatgc cgcccgcgct ttcaccgatc tcctcgaggc cgagaattca
     1261 atcggcaagg cttataatgt ggcggccgaa gagatcctct cgctcaatga ttatctgcac
     1321 ctgctcgcca ggctgatggg acgggatatc cgtttcgttc accttgacca ggagctcttc
     1381 gaccggctcc cctttagccg ccatcccggg ggtgatgtgt tccccttcaa cacccgccgg
     1441 gatgccacct tcagcctgga agagatcagc agggatctgg gctaccatac aacgccgata
     1501 gcgcagtggc tcgccactac gatagattgg ttcaccactc actacaacgg ccactccaca
     1561 ggctatgaag cccgggcgca ggagcttgca gtcattcaaa agatagacga ggaccggtcg
     1621 ccattataag acacaaggcc atcccggggt tcgccgccgc tcttgcaggt caggagcggc
     1681 gaagaggaga ggctggatag caactgggcc tcgaaactga actcgcagag gcatagccat
     1741 cctgccctct ccacactggt atctcgggat ttggcggaaa gagcagcttg gtaaatcatg
     1801 gagcggatga gatgggcaag gttctagtac tttttgacag cgaaaccggc aatacccgca
     1861 ggatggccgg atatgtggcc gagggcgcgc gcagcatcga gggcacggag gtgcggctgc
     1921 tctccgtgga tgatgcgagc agggaggatg tactctggtg cgacgggatc gccgtcggca
     1981 gcccgaccaa catgggtgtg ctctcgtgga agatgaagcg gttctgggac cgtgaaatgt
     2041 gggatctctg gagcaagatt gatggccgga tcggctgcgc tttcgcctcc tcaggcggct
     2101 ggggcggcgg cgccgagatc acctgccaat ccattttgac tgttctgatc aatttcggct
     2161 atcttgtttt cggcgcgccc gattatgtgg ctgaccgctt taccctgcat tacggcgctg
     2221 tcgccgccgg cgagccgcgc cacgagcgtg agatcgccgc ctgccggaag cttggcgaac
     2281 gccttgccta ctgggtcgcc ctctattcca aaaaatgaaa ggtcttgcca tgcgagaaaa
     2341 aattctgctc gacaccgata tcggtatgga cgtcgatgat gccgtctgcc tggcctacct
     2401 tctggctcac ccggagtgcg atctgctcgg catcaccaca gtgaccggcg aggctgtcga
     2461 acgggcaaag ctcgccagcg tgatttgccg tgcggcgggt cgagaaatac cgatctttcc
     2521 gggagcagaa gatccgctca tcgttgaaca gaagcaaaag tgcgcgcctc aggcggctgc
     2581 tctggtgcgc tgggatcatc aaacgaactt tccgaagggg gaggcggtcg agttcatgcg
     2641 caaaaccatc cgcgctcatc ccggcgaggt catcctgctc gccatcggcc ccctcaccaa
     2701 cctcggcctt ctattcgccg tcgacccgga gattcccgcc ctcctcaagg gagtggtgat
     2761 gatgggcggt tacttctttc atcgcctgcc ggaggtcagt ccggtggaat ggaatgcctc
     2821 gggcgacccc catgcctcag cgattgttta ccgcgcccgc gttcccctgc atcgctccgt
     2881 gggcctggat gtaacccggc aagtgattct gactgcggaa gaggtgcgcc ggcggttcaa
     2941 atcggaactg ctcaggcccg tgctcgactt tgccgagatc tggttcgccg gctattatcc
     3001 ctccatcacc tttcatgatc ccctggcggc cgccacaatt ttcgatgaac ggctctgcac
     3061 tttccggcag ggctctatta ctatcgaact tgaggatgca gctaatgctg gctataccca
     3121 ctggcgcgac ggtggggagg agaaccccca cgaggtggct gagagcgtcg acgccgaggc
     3181 ctatttccgc catttcttct cgatctgtgg ctgaaccagg ccgaatagta cggcggatcc
     3241 acttcaatca tggagccaac atgctcaaac accgaatcat gctcaccgcc gccatcgtgg
     3301 cactgaccct gctggtctca tgcatcgaca gggaaaataa acagggacgc gtcacgctca
     3361 ccttctggca cagttttgtc tcttcaaccc tcccggcgct ggatgatttg atcagccgat
     3421 tcgaaagtga gtaccccgcc atccacat
//
LOCUS       DAOPWU010000355         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_27952_length_3444_cov_4.702272, whole genome shotgun sequence.
ACCESSION   DAOPWU010000355 DAOPWU010000000
VERSION     DAOPWU010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27952_length_3444_cov_4.702272"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            514..714
                     /locus_tag="PKM23_13560"
                     /pseudo
     CDS             514..714
                     /locus_tag="PKM23_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
     gene            1231..2142
                     /locus_tag="PKM23_13565"
     CDS             1231..2142
                     /locus_tag="PKM23_13565"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNY92545.1"
                     /translation="MSCLKLVLKAGRRVRKAVIPAAGFGTRLFPATKAVKKELFPIID
                     GKGRAKPVILAIVEEALAAGVEQVGIIVQSQDRELFEEIFCTPPPIENFNKLSREDQQ
                     YCNYLMEIGNRITFLTQDTQEGFGHAVYCAHDWVGDEPFLLLLGDHLYASEVEKSCSR
                     QLLDLYERVGVSVVGAKINAGDEVGHFGCMTGVWKEPNSSLSITEFAEKPTPEYARQH
                     LHMEGMGEDEFLTVFGQYVLDPKIFAYLEENIRLNLRERGEFQLTSCLDRLRKEEGFV
                     GYVVKGRRFDIGNPAAYRQSVIDFAGA"
     gene            2295..2780
                     /locus_tag="PKM23_13570"
     CDS             2295..2780
                     /locus_tag="PKM23_13570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNY92546.1"
                     /translation="MILSKSCDHAIRATLYVAMHPDRGYVPIREIAAGLGISFHFLTK
                     ILQVLTQKNIMTSFKGPNGGIALARPAEEIALKEIVLAIDGEGLFRGCMVGLDHCDDD
                     HPCPLHEQWSALRGDLDELFSRTSVAHLAGRARAEGLRLTDLLPGELTKQNAGPDSSR
                     R"
     gene            2819..>3444
                     /locus_tag="PKM23_13575"
     CDS             2819..>3444
                     /locus_tag="PKM23_13575"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY92547.1"
                     /translation="MTRIIERIKKELADPQSVRRMVQWGFALLTIWIGIKFILFVHQA
                     GGSGEITITRPPGVEAFLPISALISLKFWLLTGIFNTIHPASLVLLLVIMATALLLKK
                     GFCGWVCPFGLLSEYLAAIHTWIFGRPRRLPRWLDYPLRSLKYLLLGFFAYAIFWEMG
                     VRELGAFINTPYNKVADVKMYLFFANASAFTLKVLAALLEGADVPTLR"
BASE COUNT          667 a         1122 c         1002 g          653 t
ORIGIN      
        1 cgggcaaacg gagagaaaaa agcctcacac ggggtcacgg agagcacaga gaaaaagata
       61 gtctatgtga acctataagt tattatttat cttttctttg tgtctctgtg cctttatgag
      121 agttcgcttt ttcttttttc tctctcttta tgcccggcaa gatatttttc tttgtttatt
      181 ttaccgctca acgatcactc actttcggga gaaaaattga tgaagctatt tgtgccgggg
      241 cggatctgcc tcttcgggga acacagtgac tgggcgggag ggtatcggcg gatcaacgca
      301 gccatcgaaa agggctacgc catcatcgcc ggcacaaacc aggggctgca cgccaacatc
      361 aagccccatc cctccaaatt catcttcagg gccaccctcg gcgacggcac ccggcaggga
      421 ccctacgagg tgccgatgga gcgcgaagcc ctgctcgccg aggccgaaaa gggcggcttt
      481 ttcagctatg ccgccggggt tgcctaccag atcatgacca agcaccgggt gcgcgggctc
      541 gagatcgaca actttcgcac cgacctgccg gtcaagaagg ggctctcctc cagcgcagct
      601 gtctgcgtgc tggtcgcccg cgccttcaac cgcgtctatg acctcaagct gactatccgc
      661 ggcgagatgg agctggccta tttcggcgag atcaccaccc cctcgcgctg cgggcgcctc
      721 gatcagggct gcgcctacgg caaccgcccc atcctcatgg tcttcgacgg cgaccgcatg
      781 gatgtcgagg agatctcggt gccgggcgac ctccacttca tcatcgtcga tctccacgcc
      841 agcaaggaca cccgcgagat cctcaaccag ctcaaccact gctacccctt cgccgaaaac
      901 gaggagcagc gccaggtgca ggagtacctc ggccccatca accagcgcat cgtcgcctcc
      961 gcccaggaag ccctggctgc cggcgacgcg gccgcgctcg gcgcacacat gaccgaggcc
     1021 caggccgaat tcgaccgcca tctccagccc gcctgtccca gccagctcac cgccccggtg
     1081 ctgcaccggc tgctggcctg ggagaaactg aaaccctacg tctacggcgg caagggggtc
     1141 ggttcgcagg gggacggctc cgcccagttc atctgccgcg acgaggagag ccaggccaaa
     1201 gcggtcgagc tcatcgagaa ggagatgggc atgtcctgcc tcaagctggt cctcaaggcg
     1261 ggccggcggg tgcgcaaggc ggtcatcccg gcagcgggct tcggcacgcg cctctttccg
     1321 gcgaccaagg cggtcaagaa ggaactcttc ccgatcatcg atggcaaggg gcgcgccaag
     1381 ccggtgatcc tcgccatcgt cgaggaggcc ctcgccgcgg gggtggaaca ggtcggcatc
     1441 attgtgcaga gccaggaccg cgaactcttc gaggagatct tttgcacccc gccgccgatc
     1501 gagaatttca acaagctctc gcgcgaggac cagcagtact gcaactattt gatggagatc
     1561 ggcaaccgca tcaccttcct gacccaggac acccaggagg gcttcggcca tgccgtctac
     1621 tgcgcccatg actgggtcgg cgacgagccc ttcctgctgc tgctcggcga ccacctctac
     1681 gcctcggagg tggagaaaag ctgctcgcgc cagctcctcg acctctacga gcgggtcggg
     1741 gtcagcgtgg tcggggccaa gatcaatgcc ggcgatgagg tgggccattt cggctgcatg
     1801 accggggtgt ggaaggagcc caactcctcc ctttccatca ccgagttcgc cgaaaagccg
     1861 acgcccgaat acgcccgcca gcacctccac atggagggga tgggcgagga tgaatttctc
     1921 accgtcttcg gccagtatgt actggatccc aaaatcttcg cctatctcga ggagaacatc
     1981 cgcctcaacc tgcgcgagcg cggcgagttc cagctcacct cctgcctcga ccggctgcgc
     2041 aaggaggagg ggttcgtcgg ctatgtggtc aaggggcggc gcttcgacat cggcaaccct
     2101 gccgcctacc gccagtcggt gatcgatttc gccggtgcat agagggggga ttgctgcggc
     2161 cggactaact ctcgcgccca cccgggggtt gctgccggat cggggcaaca tctccaaaaa
     2221 atccttgcat tccccataaa aatggattat atttaagaca taagagtctt ttaactcaat
     2281 tagcgagtcc gcccatgatc ctctccaaga gctgcgacca cgccatccgc gccacccttt
     2341 acgtcgccat gcatcccgac cggggatatg tgccgatcag ggagatcgcg gcgggcctgg
     2401 ggatctcgtt tcatttcctc accaagatcc ttcaggtgct gacgcagaag aacatcatga
     2461 cctccttcaa ggggcccaac ggcgggatcg ctttggcgcg gccggcggag gaaatcgccc
     2521 tcaaggagat cgtcctggcc atcgatggcg agggcctctt ccggggctgc atggtcgggc
     2581 tcgaccactg cgacgacgat catccctgtc ccctgcatga gcagtggagc gctctgcgcg
     2641 gcgatctgga cgagcttttc agccgcacat cggtcgccca cctcgccgga agggcgcgcg
     2701 cggagggctt gcgtctgacc gacctgctgc cgggagagct gacaaaacaa aacgcaggcc
     2761 cggattcgag ccggcgttag cagagagaac aaggacaggt atcgatcgca ttatgaagat
     2821 gacaaggata atcgaacgga ttaaaaagga gctggccgat ccgcagtcgg tccggcgcat
     2881 ggtccagtgg ggctttgccc tgctgacgat ctggatcggg atcaaattca tcctcttcgt
     2941 ccatcaggcc ggcggcagcg gcgagatcac catcacccgc ccccccggag tcgaggcctt
     3001 tctgccgatc agcgccctga tcagcctcaa gttctggctc ctgaccggga ttttcaacac
     3061 catccacccg gccagtctgg tgctgctgct ggtcatcatg gccaccgccc tgctgctcaa
     3121 gaagggcttt tgcggctggg tctgcccctt cgggctgctc tccgaatatc tggccgcaat
     3181 ccacacctgg atcttcggcc ggccgcggcg tctgccccgc tggctcgact atcccctgcg
     3241 cagcctgaaa tacctgctgc tcggcttttt cgcctacgcc atcttttggg agatgggggt
     3301 gcgcgagctc ggggccttta tcaacacccc ctacaacaag gtcgccgacg tcaagatgta
     3361 cctctttttc gccaatgctt cagccttcac cctcaaggtg ctcgccgccc tgctcgaagg
     3421 tgccgacgtg cccaccctgc gcga
//
LOCUS       DAOPWU010000356         3436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_28034_length_3436_cov_3.156463, whole genome shotgun sequence.
ACCESSION   DAOPWU010000356 DAOPWU010000000
VERSION     DAOPWU010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28034_length_3436_cov_3.156463"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            605..1345
                     /locus_tag="PKM23_13580"
     CDS             605..1345
                     /locus_tag="PKM23_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNY92548.1"
                     /translation="MKAAILTDLTKCVGCEACALACKEINDLPRDSANHMLTAYTWSA
                     MEHRNGVNIRRQCMHCEEPACASACPVGALSKTPEGAVVYDGRKCIGCRYCMIACPFG
                     IPKYQWDKPIPLIQKCIFCYEKALKAGEQPACTAACPTGATIFGERQALIAEAQQRIS
                     STPEKYQPHIFGLKEAGGTSVLYTSAVPFAELGFPANLIEESYPNLTWKVLNKIPGIV
                     TTGTAFLAGLWWIVNRRIKLQEKPDETL"
     gene            1401..2957
                     /gene="hybB"
                     /locus_tag="PKM23_13585"
     CDS             1401..2957
                     /gene="hybB"
                     /locus_tag="PKM23_13585"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HNY92549.1"
                     /translation="MKLKLPKPSFWLITLAVIWAAGIISVVYRFYNGLGAATNLSDQF
                     PWGIWIGFDILIGVALAAGGFAVCATIYIFNLKEYRPIGRPTVLTAFLGYVLVIVALL
                     VDLGKPYNVWHVITMWNHHSVMFEVGWCVMLYTTVLFLEFLPAFFERFRMVFWQDLFH
                     RILIPLVIAGVILSTLHQSSLGSLYLIVPTKLHPIWYSGMLPVFFFISALAGGLAMVI
                     FESYLSARAFGKGIEHRLLAQISRVIAVVLMIYLVLRLQNMVERGAFKHVFDGSSEGI
                     FFLIEIILGYITPMMLFFNARIRESKAGLFFGAVLTLFGLVMNRLNVAITGMARSAGV
                     EYLPSWMEVAVTLSVIAAGLFIFRLAAKYLPVFPREEKTGVHQPPLIEAGRSGKLAMG
                     LLAGLLILVLAGFGYSLAVTRPNQSADAPQAAQAIAAESLEFPAPIEFVQSEESPGQV
                     IFNHESHVDTSAPNCKNCHAGLFSIRASKAGAAAQTRAVGHDQCGTCHNGEKAFNYEE
                     SCDGCHMSTE"
     gene            3017..>3436
                     /locus_tag="PKM23_13590"
     CDS             3017..>3436
                     /locus_tag="PKM23_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92550.1"
                     /translation="MKEIIPKYSGQANRVWPEGESACIWMQAGLVAFKLCDRAFACAG
                     CPFDELMRSGAARPAEEGSSAGEGDRQRVTGLGPESQESPLPSLHFDPATWYGAGFWY
                     MRPRGSRLFEIGLNENGTIFLPPVREVILPRRGASLTT"
BASE COUNT          666 a         1039 c          926 g          805 t
ORIGIN      
        1 taacggccgg gtacccccaa atctggattc cacctgacac ctggcggcgc gcccctccct
       61 ggatacggct tccacccgac gcccggggcc gcttgcccca gtttcctcct gaccatttcc
      121 agcatgcttc agctcacccc gataaatgtt tatatattaa acataacgcg caaacaattc
      181 gagacagtga tgctgtttta ttaaacagtc attattaatt gtaatttagt gaaatgtaat
      241 caaaaccgct cacaattgct ttacattgtt gatttattaa tcaattgata gtaatatttg
      301 ttcacattat gtccataatg ttctgtttca attaattgat aatacatgct ttaatgcact
      361 actgaaagaa cagcttccct ttggcatgca agttgcagaa gcgaggccga gcgccgatct
      421 ggcgcccgca aaccatccgg agtcttccgg aaaacgatac agacaaaccg aggaatccag
      481 tatgactgta ctgcttttgc tcgcgctggc cctggctttc atcctggccg attatgtggt
      541 tcaactgcgc caggccaaga agaaatccta aaaaatctgc gcataaccca acaggaggat
      601 gcggatgaag gccgctattc taaccgattt aaccaaatgt gtggggtgcg aagcctgcgc
      661 cctggcatgc aaggagatca acgatctgcc cagggattca gccaaccaca tgctgaccgc
      721 ctacacctgg agtgcaatgg agcaccgcaa cggtgtgaac atccgccgtc aatgcatgca
      781 ctgcgaggag cccgcctgcg cctccgcctg tccggtcggc gcgctctcca agacgcctga
      841 gggcgcagtg gtctacgacg gccgcaagtg catcggctgc cgctactgca tgatcgcctg
      901 ccctttcggc attccaaaat accagtggga caagccgatt cctctcatcc agaagtgcat
      961 tttctgttat gaaaaggccc tcaaggccgg agagcagccc gcctgcacgg cagcctgccc
     1021 gaccggtgcg accatcttcg gtgagcgtca ggccctgatc gcggaggccc aacagcgcat
     1081 ctcctccact cctgagaaat accagcccca catctttggc ctcaaggagg caggcggaac
     1141 ctcggttttg tacacctctg ccgttccctt tgccgagctg ggattccccg ccaacctcat
     1201 cgaggagtcc taccccaatt tgacctggaa ggtgctcaac aagattcccg gcattgtgac
     1261 gaccggcacg gcttttctgg ccggactgtg gtggatcgtg aacaggagaa tcaagctgca
     1321 ggaaaagcct gatgaaacgc tctagggcgg cccgttgccg gaccggtctc tcatgaactt
     1381 gttcacctac tgaggaacct atgaaattaa aacttcccaa accaagtttc tggcttatca
     1441 ccctggctgt catctgggcg gcagggatta tctctgtcgt ctaccgtttt tataacgggc
     1501 tgggtgctgc caccaatctg tcggaccagt ttccctgggg catctggatc ggttttgaca
     1561 tcctgatcgg ggttgcgctc gctgcgggcg gatttgccgt ttgcgcgacc atctatatct
     1621 tcaacctcaa ggagtaccgg ccgatcggcc gtcccaccgt tctgaccgct tttctgggct
     1681 atgtcttggt aatcgtcgcc ctgctcgtcg atctgggcaa gccctacaac gtctggcatg
     1741 tcatcacgat gtggaatcac cattcggtca tgtttgaggt gggctggtgt gtgatgctct
     1801 acaccacggt tctttttctc gaatttctgc ccgccttttt tgagcgtttc cgcatggtct
     1861 tctggcagga tctttttcac cgcatcctca tccctctggt catcgccggg gtgattcttt
     1921 cgaccctgca tcagtcctct ttgggttcgc tttatctgat cgtcccgacc aagctgcatc
     1981 ccatctggta ttcggggatg ctgccggtct ttttcttcat cagcgccctg gcgggcggtc
     2041 tggcgatggt tatcttcgag tcctatctga gcgcgcgcgc tttcggcaag gggatcgagc
     2101 accgtttgct ggcgcagatc agccgcgtga tcgcagtcgt gcttatgatc tacctggtgc
     2161 tgcggctgca gaatatggtc gagcgcggtg cattcaagca tgtttttgac ggctcgagtg
     2221 aggggatctt ttttctcatc gaaatcattc tcggctacat cacgccgatg atgctctttt
     2281 tcaacgccag gatccgcgaa agcaaggccg gtctcttttt cggcgcagtg ctgaccctgt
     2341 tcggtctggt gatgaaccgg ttgaacgtgg cgatcacagg catggcgcgc tccgccggcg
     2401 tggagtatct gccgagctgg atggaggttg cggttaccct ttccgtcatt gccgccggcc
     2461 tcttcatctt ccgtctcgcg gctaaatacc tgccggtttt cccgcgcgag gagaaaaccg
     2521 gcgtgcacca gcctcccctc attgaggccg gccgctcggg caagctggcg atgggcctgc
     2581 tcgccggact gttgatcctt gttctggccg gtttcggtta ctccctggct gtgaccaggc
     2641 ccaaccagtc cgcggatgcc ccccaggccg ctcaggccat cgctgcagag agtctggaat
     2701 tccccgcacc gatcgagttc gtccaatcgg aggagagccc cggacaggtc atcttcaacc
     2761 acgagtcgca tgtggatacc agtgcgccca actgcaagaa ctgccacgcc ggtctctttt
     2821 caatccgcgc ctccaaggct ggtgccgccg cccaaacccg ggcggtcggc catgatcagt
     2881 gcggcacctg ccacaatggc gaaaaggcat tcaactatga agagagctgc gacggctgtc
     2941 acatgagtac ggagtaatgg gccgggcggg gaaccacctc ggccatcctg ctctccactt
     3001 ccgatccgtg gaaccgatga aagaaatcat tccgaaatat tccggccagg ccaaccgggt
     3061 ctggccggag ggcgaatccg cctgcatctg gatgcaggcg ggtttggtcg ccttcaagct
     3121 ctgcgaccgc gccttcgcct gtgccggctg tcccttcgac gagctgatgc gcagcggtgc
     3181 ggcccgtccc gcggaagagg ggagctctgc cggggagggc gatcggcagc gcgtcaccgg
     3241 attggggcct gaaagccagg agtcccccct cccatctctc catttcgatc ccgccacctg
     3301 gtacggggcc ggtttctggt atatgcgtcc acggggcagc agactttttg aaatcggact
     3361 gaacgagaac gggaccatct ttcttccgcc cgtgcgggag gtcattctgc cccgccgggg
     3421 cgcctccctc accacc
//
LOCUS       DAOPWU010000357         3435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_28044_length_3435_cov_3.870710, whole genome shotgun sequence.
ACCESSION   DAOPWU010000357 DAOPWU010000000
VERSION     DAOPWU010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28044_length_3435_cov_3.870710"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PKM23_13595"
     CDS             complement(<1..659)
                     /locus_tag="PKM23_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92551.1"
                     /translation="MRHSFLLGLAGLLPAIFAPVLLAADLIRIQPVTDKILVLYFSEG
                     HIDYFGIGQERSRGNRVYYSRLDTAAAAVLSNYIISSPDDIRYQTGQGPIAIGRKAKG
                     ESFNDLYAAPMPRVLMAHWIYLELPFSMNQGGTYTVKLNNLASNVNAYTFLFDAFSLR
                     SPAIHLNQIGMVPGAPKYAYLSQWMGSFDTPDHPAGALNLDRYYYAPFHLVRSSDGAI
                     V"
     gene            complement(718..888)
                     /locus_tag="PKM23_13600"
     CDS             complement(718..888)
                     /locus_tag="PKM23_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92552.1"
                     /translation="MHGWDQTVNNSGNTRVGHNTPVKNLYMAGAWSRPGHGYGAVIPS
                     GVECFAEIVKNR"
     gene            complement(962..1375)
                     /locus_tag="PKM23_13605"
     CDS             complement(962..1375)
                     /locus_tag="PKM23_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HNY92553.1"
                     /translation="MRGPYLYDYAIVRIVPRVEREEFVNVGVILSCPQKKYLEARIEL
                     DEGRLVALVPGLDLELLKSQLAIYPLICRGGEEAGPIGRLSQRERFNWLIAPKSTIIQ
                     TSPVHTGWCEHPDLVLEHLLNTMVRPADYRNAARG"
     gene            complement(1353..2129)
                     /locus_tag="PKM23_13610"
     CDS             complement(1353..2129)
                     /locus_tag="PKM23_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HNY92554.1"
                     /translation="MRRVEATRYVTPLKEGGSLPAIVEADDDGLYVLKFRGAGQGPKA
                     LIAELVAGEIARAAGLPVPEIVFVHLEAELARTEPDPEIHDLIRMSAGLNLALDYLPG
                     SIMFSPVLHPVSETLASEIVWLDAFVTNIDRTPRNTNMLMWHKQLYLIDHGASLYFHH
                     SWYHYLERTSDPFPLIKDHVLLPSAGALRQAGEKLKAAIGPDVVEEVVGMIPDDWFDG
                     HSPFSSPGETRKAYIVYLKKRLEESESFVEEAMRARSVLV"
     gene            complement(2147..2578)
                     /locus_tag="PKM23_13615"
     CDS             complement(2147..2578)
                     /locus_tag="PKM23_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNY92555.1"
                     /translation="MYRISIRTGRRCDIVDITPSIRTYLREIGAESGTVTVFCPHTTA
                     GLTVNENADPDVRRDLNAFLNRAIPREGDYAHSEGNSDAHIKSTLTNSAQMLIVEGGE
                     VMLGTWQGVFFMEYDGPRNREFWLQFTGERTAAPRREGEGR"
BASE COUNT          786 a          982 c         1002 g          665 t
ORIGIN      
        1 aaaacgatcg caccatccga gctccgcacc aggtgaaagg gggcgtaata atagcgatcg
       61 agattgaggg cgccagcggg atggtcaggc gtgtcgaagg agcccatcca ctgcgagaga
      121 taagcgtatt tgggcgcccc cgggaccatc ccgatctggt tgaggtgaat tgcgggcgag
      181 cgcagggaaa aggcgtcgaa gaggaaggta taggcattga cattcgaggc gaggttgttc
      241 agcttgaccg tgtaggtgcc gccctgattc atgctgaagg gcagctcgag gtagatccag
      301 tgggccatca gcaccctggg catcggcgcg gcgtagagat cgttgaagga ttcgcccttg
      361 gccttgcgtc cgatagcgat cggtccctgg ccggtctgat agcggatatc gtccggactg
      421 gagatgatat agttggagag aaccgcggcg gcagcggtgt ccagccgcga gtaatagacg
      481 cgattgccgc ggctccgctc ctggccgatg ccgaaataat cgatgtgccc ttcggaaaaa
      541 tagagcacca ggattttgtc cgtcaccggc tggatgcgaa tcagatcggc ggccaaaagc
      601 accggtgcaa aaatggccgg cagcaggccg gccaggccaa gcagaaaact gtgcctcatt
      661 ttgagaactc cagaatggta aaagggcgga aacaccggcc tccgcaagcg gttgatttca
      721 ccggtttttg acgatctccg caaaacactc cacgccgctg ggaataacgg cgccatagcc
      781 gtgacccgga cgcgaccatg cccccgccat atagaggttt ttgaccggcg tattgtggcc
      841 gacgcgggta ttccccgaat tgttgacggt ctgatcccaa ccgtgcatgt tgatgaaagt
      901 tacactactg atagaacagg tataccggtc gcaaaggagc ttgcctgact tgcggggccg
      961 atcaccccct ggcggcattg cggtaatctg ctggcctgac catggtgttg agcagatgct
     1021 ccagcaccag gtcagggtgc tcgcaccagc ccgtgtgcac cggcgaggtc tggatgatgg
     1081 tgcttttggg cgcgatgagc cagttgaagc gttcgcgctg tgacagcctg ccgatgggtc
     1141 ccgcctcctc tccgccacgg cagatcaggg gatagatcgc caactggctc ttcagcagct
     1201 cgagatccag gcctggaacg agcgcgacga gacggccttc atccagctcg atgcgcgcct
     1261 cgagatactt tttttgcggg caggagagaa tcaccccgac attgacgaac tcttcgcgct
     1321 cgacccgggg gacgatgcgc actatcgcgt aatcatacaa gtacggaccg cgcacgcatc
     1381 gcctcctcga caaaggattc cgattcctcc agccgtttct tgagatagac gatataggcc
     1441 tttcgggtct ctcctggaga cgaaaaaggc gagtggccgt cgaaccagtc atccggaatc
     1501 atccccacca cctcctcgac cacatcaggc ccgatggccg cttttaactt ttccccggcc
     1561 tgccgcagcg caccagccga cggcagcagc acatggtctt tgatgagggg aaagggatcg
     1621 ctggttctct ccagataatg gtaccaggag tgatgaaaat agagggaggc accatggtcg
     1681 atgaggtaga gctgcttgtg ccacatcagc atgttggtat tgcgcggtgt gcggtcgatg
     1741 ttggtgacaa aggcatccag ccagacgatc tcggaagcca gggtttcgga gaccgggtgc
     1801 agcaccggac tgaacatgat ggagcccggc aggtaatcga gggcgagatt gagtcctgca
     1861 ctcatgcgga tgagatcatg gatctccggg tcgggttcgg tgcgcgccag ctccgcctcc
     1921 agatggacaa agacgatctc gggcacaggc aatccggcgg cgcgcgcgat ctctccggcg
     1981 accagctcgg cgatcagggc cttcgggccc tgtcccgcgc cacggaactt taatacatac
     2041 aggccgtcat catcggcctc aacgatggcc ggcagtgagc cgccctcctt caatggcgtc
     2101 acgtagcgtg tagcttccac ccttctgatc gtcaatgctt ctcctttcat ctcccttcgc
     2161 cctctcgccg cggcgctgcg gtgcgctcac cggtgaactg aagccagaac tcacggttgc
     2221 gcggcccgtc gtactccatg aagaaaaccc cctgccaggt gccgagcatc acctctcccc
     2281 cctccacgat cagcatctgc gcggagttgg tgagggtgct cttgatgtgg gcatccgagt
     2341 tgccctcgct gtgggcatag tccccctccc ggggtatggc ccggttgaga aaggcgttga
     2401 gatcgcggcg cacatcggga tcggcatttt cgttgacggt gaggccggcc gtggtatggg
     2461 gacaaaaaac cgtcaccgtc ccgctctccg cgccgatttc gcgcaaatag gtgcgaatgg
     2521 acggggtgat atcgacgatg tcgcagcggc ggccggtgcg aatggatatt ctatacatgc
     2581 ctgcgttctt tcacaagggt cacaatgcgg gaaaagaaag tacctgatag ggaaaatagc
     2641 caaccggtcc cttaaaaaca agcggaaatt gagaaatcag caagcgggat aggtgcagga
     2701 agagagtaat ccctcagtcc gctgcctgac cctgatcaat cttcgcggct gccaggagtg
     2761 cgagccggat ctccggcacg ccagggccgc gggttcgagc cccgtcggcc cggccagcat
     2821 aagtaaagta aaatcaaaag agagtcctgg atttacagaa cgaatgagat tgaatagggg
     2881 aatatcaggt tatcaactgc agcaattgct ttcggcatgg tcaataccca ttacaggtgg
     2941 ctaaccgtca caaagacttc gtccgcccgc cgcatgccca gcagcagcgc ccgcttctga
     3001 tcgcaagagg gacagcagca gcgctgacgg caggaaaacg ccacgaaaaa ctccttacca
     3061 caatccggac agcgaacccg taccaatccc tcccgcaggt ctccgcactt tacatacttg
     3121 tctatgacgg tgcgaatggc cgaccgccaa tagccctatt tcggctggaa ccgttcctcg
     3181 taaacccact cgaactcgtc aaaatggtca cgcaccactc tataaaaggg cgaagcctcg
     3241 ggattgcgag gacggtacat ttttacccca ggagcacaca cgccattccc gggacaacta
     3301 aagcttaggg tatagcctat gctataaaat tactgaaaac gcctcaagga acaaaatcac
     3361 cggcgcctca tgcactgaga ccggtactct ataaagggct tgaccataaa tcaatagtaa
     3421 gctatattta aagaa
//
LOCUS       DAOPWU010000358         3416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_28227_length_3416_cov_4.504612, whole genome shotgun sequence.
ACCESSION   DAOPWU010000358 DAOPWU010000000
VERSION     DAOPWU010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28227_length_3416_cov_4.504612"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..466
                     /locus_tag="PKM23_13620"
     CDS             212..466
                     /locus_tag="PKM23_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY92556.1"
                     /translation="MAEPIGLERAKIVVREDECKGCGLCIEACPEQVLFVHKHLNRLG
                     YHPTGYKGEGCTGCGICFYQCPEPGAITVYKKGYVGEEVV"
     gene            468..1517
                     /locus_tag="PKM23_13625"
     CDS             468..1517
                     /locus_tag="PKM23_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNY92557.1"
                     /translation="MAKQLIKGNEAVVKGAILAGCRFYFGYPITPASEVAEAAAYYLP
                     KVGGTFIQAESEVAAINMVYGAASADVRAMTASSSPGISLKMEGLSYCAGAELPFVLV
                     DIMRAGPGLGNIAPEQGDYNQIIKGGGHGNYKVVCLAPNSAQEMCDLTILAFDLADKY
                     RIPAVLLADGFIGQMEEPVEFPPARTSLPDKPWAIHSDADDEKRLITSINLEPELLEK
                     HNLHLQEKYAEITRNEIRFEDYQTADAELVIIAYGVVSRIVQTTIDDMRAAGRKVGMM
                     RPISLWPFPSAHIVELGDQVKQFLVVELSDGQMVDDVRLALNGKRPVHLHNRMGGMVP
                     SPEEVQERALKILEG"
     gene            1521..2954
                     /locus_tag="PKM23_13630"
     CDS             1521..2954
                     /locus_tag="PKM23_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNY92558.1"
                     /translation="MAEKVLAKAESFFDIYDRNPSPSKDVTHYCPGCGHGVLHKLIAE
                     AIDDFGIRERTVLFSPVGCSVFAYYYFRTGNIQCAHGRAPAAATGFKRTHPESIVISY
                     QGDGDLAAIGTAEIIQAANRGENFTVFFVNNAIYGMTGGQLAPTTLVGQKTTTSPYGR
                     NPANEGYPIKVAEMISTLDAPVYVERVAITDAKNTARVRRAVRKGLQNQIDNKGFSLI
                     EVLSVCPSNWKMDPIQSKQWLSDNMIQQFPLGIFRDRTGEVPAAENKRHIFHVDGLRE
                     ALELPVETESTIKVAAPAPEFQDPRIKLAGFGGQGVLMLGLMLAEAGMHAGYHVSWIP
                     SYGPEMRGGTANCHVNLSHRRIGSPTVSRPTLLVAMNLPSLERFENEVVPGGLIIYDT
                     AMVTRAPKRTDVKALGIPASTIADELGNTRGANMVILGAYIGYTSILPKEAIFAAMPS
                     LIKRKNLIPLNEQAVEKGMEFVRNLRG"
     gene            2964..>3416
                     /locus_tag="PKM23_13635"
     CDS             2964..>3416
                     /locus_tag="PKM23_13635"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNY92559.1"
                     /translation="MTISQSNPGHPSLKQRIFSDLLPFVAKPGRYIGNEFNMIRKDPA
                     AAEVRVALVFPDVYEVGMSYLGHPILYHILNQQPHIYAERVFAPWLDMAAQMREKGVE
                     LFSLETFSPLRDFDLIGFTLQYELNYTTNIDLIDLAGLPLLAADRQEGP"
BASE COUNT          677 a         1081 c         1038 g          620 t
ORIGIN      
        1 ccaaggccgg gtgggaacga gggggggact gcgctagcgg ccggggccgg gtgggcgcga
       61 gggggggact gcgcggatgc cggggcgccg gagagggtgg cgagaatctc cttgcaagtt
      121 agcctaaaaa tggttaaaat gaggcgcgaa aatgggcgct gcggacggtg ttccacgccc
      181 cagcgcgggt cataacccag gaggtttaag tatggcggaa cccatcggac tcgagcgtgc
      241 caaaatcgta gttcgtgaag atgagtgcaa aggctgcggc ctctgcatag aagcctgccc
      301 ggaacaagtt ctgttcgtcc acaaacatct caatcgtctc ggttaccacc ccaccggcta
      361 caagggtgag ggctgcaccg gctgcggcat ctgtttctac cagtgccccg agcccggcgc
      421 catcacagtc tacaagaagg gttatgtcgg agaggaggtg gtctgagatg gcgaaacagc
      481 tgatcaaggg gaatgaagcc gtcgtcaagg gggccatcct ggccggctgc cggttttatt
      541 tcggatatcc gatcacccct gcctccgagg tggccgaagc ggctgcctac tatctgccca
      601 aggtcggggg gaccttcatc caggctgaaa gcgaagtggc ggcgatcaac atggtttacg
      661 gtgctgcctc ggccgatgtg cgcgccatga ccgcctcctc ctcgcccggc atcagcctga
      721 agatggaggg gctctcctac tgcgcgggcg ccgagctgcc ctttgtgctg gtcgatatca
      781 tgcgcgccgg ccccggactg ggcaacatcg ccccggaaca gggggattac aaccagatca
      841 tcaagggcgg tggccatggc aattataaag tggtctgcct ggcgcccaac agcgcccagg
      901 agatgtgcga tctcaccata ctcgccttcg acctggccga caagtaccgc atcccggccg
      961 tcctcctcgc cgacggcttt atcggacaga tggaggagcc ggtggagttt ccgcccgcgc
     1021 gcacctcgct gccggacaag ccctgggcta tccacagcga tgcggacgac gaaaagcgtc
     1081 tcatcacctc catcaacctc gaacccgaac tgctcgaaaa gcacaacctc cacctgcagg
     1141 aaaagtacgc cgagatcacc cgcaacgaga tccgcttcga ggattatcag accgcggatg
     1201 ccgagctggt catcatcgcc tatggcgtgg tctcccgcat cgttcagact actatcgatg
     1261 atatgcgcgc cgcaggccgg aaggtgggca tgatgcgtcc catctcgctc tggcccttcc
     1321 cgagcgccca catcgtcgaa ctgggcgatc aggtcaaaca attcctggtg gttgaactct
     1381 ccgatggaca gatggtggat gacgtgcgcc tggccctcaa cggcaagcgg ccggtgcacc
     1441 tccacaaccg gatggggggg atggtcccct cgccggaaga ggtccaggag cgggccctga
     1501 aaattctgga gggttgagcg atggctgaaa aagttctagc aaaagcggag agttttttcg
     1561 atatctacga ccgcaatccc agcccgagca aggatgtgac ccattactgc ccgggctgtg
     1621 gtcacggtgt gctgcacaag ctgatcgccg aggcgatcga tgatttcggc attcgcgaac
     1681 gtaccgtcct cttcagtccg gtgggctgca gcgtcttcgc ctactactat ttccgcaccg
     1741 gcaatatcca gtgcgcccac ggccgcgcgc ccgcggcggc gaccggcttc aagcgcaccc
     1801 atcccgagag catcgtcatc agctatcagg gtgacggcga tctggcagcc atcggcacgg
     1861 ccgagatcat ccaggcggcc aaccgcggcg agaatttcac ggtctttttc gtcaacaacg
     1921 ccatttacgg catgaccggc gggcagctcg ccccgaccac cctggtcggg caaaagacca
     1981 ccacttcgcc ctacggccgc aatccggcca atgagggcta tccgatcaag gtggccgaga
     2041 tgatctccac cctcgacgcc ccggtctatg tcgagcgggt ggcgatcacc gacgccaaga
     2101 atacggcccg ggtgcgccgc gctgtgcgca aggggctgca gaaccagatc gacaacaagg
     2161 gtttttccct gatcgaggtg ctctcggtct gcccctcgaa ctggaagatg gacccgatcc
     2221 agtccaagca gtggctgagc gacaacatga tccagcagtt cccgctcggg atcttccgcg
     2281 accgcaccgg ggaggtcccg gcggcggaga acaagcgcca catctttcat gtggacggcc
     2341 tgcgcgaggc cctcgagctt cccgtcgaga cggagagtac gatcaaggtg gccgcgccgg
     2401 cccccgagtt tcaggatccc cgcatcaagc tcgcgggatt cggcggccag ggcgtgctca
     2461 tgctcggact catgctggcc gaagcgggca tgcacgccgg ctaccacgtc tcctggatcc
     2521 ccagctacgg cccggagatg cgcggcggca ccgccaactg ccatgtcaac ctttcccacc
     2581 ggcgcatcgg ttcgccgacg gtgtcgcggc cgaccctgct ggtggcgatg aatctgccct
     2641 cgctcgagcg tttcgagaac gaggtggtgc cgggcgggct gatcatctac gacaccgcca
     2701 tggtcacccg ggcgcccaaa cgcaccgacg tcaaggcgct gggcattccg gcttcgacca
     2761 tcgcggacga actcggcaac acccgcggcg ccaatatggt catcctgggg gcctatattg
     2821 gctatacctc gattctgccc aaggaggcca ttttcgccgc catgccctcg ctgatcaaac
     2881 ggaagaacct cattccgctc aacgagcagg cggtcgaaaa ggggatggaa tttgtgcgca
     2941 acctgcgggg ttaggacaag ggcgtgacca tttcgcagtc caatcccggc catccgtccc
     3001 tcaaacagcg catattcagc gatttgctgc cgttcgtggc caaacccggc cgctacatcg
     3061 gcaatgaatt caacatgatc cgcaaggacc cggcggcggc ggaggtccgc gtcgccctgg
     3121 tctttccgga tgtttacgag gtcggcatgt cctatctggg ccatccgatc ctctaccaca
     3181 ttctcaatca gcagccccac atctatgccg agcgcgtctt tgcgccctgg ctggacatgg
     3241 ctgcacagat gcgggaaaag ggagtggagc ttttttcact cgagaccttt tcaccgctgc
     3301 gggattttga tctcatcggt tttacgctcc agtatgagct caattataca acgaacatcg
     3361 atctcatcga tctggccggc ctgcccctgc tggcagccga ccggcaggag gggccc
//
LOCUS       DAOPWU010000359         3411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_28282_length_3411_cov_4.640346, whole genome shotgun sequence.
ACCESSION   DAOPWU010000359 DAOPWU010000000
VERSION     DAOPWU010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28282_length_3411_cov_4.640346"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PKM23_13640"
     CDS             <1..1166
                     /locus_tag="PKM23_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92560.1"
                     /translation="VQWLIVPDESQAIPAFARRYNLSCSTCHAPIPKLKPYGADFAGD
                     GFVIVENEKERDYVTAGDEMLWLNKTFPIGIRFDAYAVTNSEGDVKSDLQSPWGLKLM
                     SGGALAKNIGYYFYFFMSERGEVAGIEDAYVHFNNIFGIPLDVLVGQFQTSDPLMKRE
                     LRLTFEDYMIYKRKVGHSNANLTYDRGIMLLYGLEQTSTDFAFTLTNGNGKGLANEES
                     GAFDVDANKNIGVRLLQGITDKISIGGYYYFGEETMHDIGGERKNKVTYIGPDVNVTL
                     GPVEVTAQYLQRKDTNPWFAEAEAETETSGIVLEAVISPQLDRSRYYFTALYNSIDQE
                     QGIGTLSTNSSLYETATLGATYLVTRNLRLMAEFTRNLEMDANRMLVGLVSGF"
     gene            complement(1266..2033)
                     /locus_tag="PKM23_13645"
     CDS             complement(1266..2033)
                     /locus_tag="PKM23_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNY92561.1"
                     /translation="MEQLEGKICVEAALRARQRRIHLLVLRNSLHAETSAPLIELAQA
                     RQIPVKLVSPDELDAMAHGHTHGGVLALCSPRPHLAIDQLFKHLNSCGFPLFLLLLEG
                     IDDSQNLGFVLRTAEACGVDAVLLKKHLWDFDGAAVSRASSGAFERLPLCVFDQAESL
                     LPRISSLGVRIYGCIANAQRTIYQVDLTRSVLIALGGEKRGLSAAVRGYCDRFIKIPM
                     LSAAGSLSLSHASAILLAEVMRQRLSRDETFQDPNPA"
     gene            complement(2036..2935)
                     /locus_tag="PKM23_13650"
     CDS             complement(2036..2935)
                     /locus_tag="PKM23_13650"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HNY92562.1"
                     /translation="MTIPHKIAGLGELLWDIYDDRRHAGGAPANFALHARRCGADALL
                     LSRVGNDQAGRDLKALLARNGVDVSAIQTSPDKPTGSVRVHLGPAGAPRFECSRDTAF
                     DQLECDQVWRTLLPSLDALLYGVLAQREETSRNAIRACLHHADKALLLFDLNLRGWND
                     STRDIVLNSLSLCDIVKMNETELAILQQALDAGKMDRQDFLLSLLHSYNIKMAAVTLG
                     SRGCWLLTPDQNLHHPGFRVRAVDTTGCGDAFAAGLIWSILNKASLEEMAEFANRLGA
                     FVATRQGAMPEWSQDDLAALGEA"
     gene            complement(2932..>3411)
                     /locus_tag="PKM23_13655"
     CDS             complement(2932..>3411)
                     /locus_tag="PKM23_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNY92563.1"
                     /translation="SIHNELNEVVEETIPKSQRVTDAQTAFLLLDMLTGVVEKGGTGS
                     AVRSYGFYRPCAGKTGTSNDYRDAWFVGFTPELAVAVWVGYDDNRSMRDSRGKGITGS
                     RAALPIWALFMKNAFASAQITNFQVPDRITFATIDPRTGSGRMPGGPSLTVALQAEE"
BASE COUNT          740 a          939 c         1058 g          674 t
ORIGIN      
        1 gggtgcagtg gctgatcgta ccggatgaga gccaggccat ccccgcgttt gcgcggcgct
       61 acaacctttc ctgctccacc tgtcacgccc ccatccccaa gctgaaaccc tacggtgctg
      121 attttgccgg tgacggcttt gtcatcgtcg aaaacgagaa agaacgcgac tatgtaaccg
      181 ccggggatga gatgctgtgg ctgaacaaga ccttcccgat cggtatccgc tttgacgcct
      241 atgccgtgac caattctgag ggcgatgtca agagtgattt gcaatccccc tggggcttga
      301 aactgatgtc cggcggggcc ctggctaaaa atattggcta ctatttttat ttcttcatga
      361 gcgaacgcgg cgaagtggcg gggatcgagg atgcttatgt tcatttcaac aatatcttcg
      421 gcatccccct agatgtcctg gttggccagt ttcagaccag cgatccactg atgaagcgcg
      481 aactgcggtt gacttttgaa gattacatga tttacaaacg caaggtcggt cactccaacg
      541 ccaatctcac ttacgatcgg ggcatcatgc tgctctatgg tcttgaacag accagcaccg
      601 atttcgcgtt taccctgacc aatggcaacg gcaagggtct ggccaacgaa gagagcggcg
      661 ccttcgatgt cgatgcaaac aagaacatcg gcgtgcgctt gctccagggc attacagaca
      721 agatcagcat cggcggctat tattatttcg gtgaagagac catgcatgac atcgggggtg
      781 agagaaaaaa caaggttact tacatcggcc cagatgtcaa cgtcactctc ggcccggtcg
      841 aagtcactgc acaatacctg cagcgcaagg acaccaatcc atggttcgct gaagccgaag
      901 cggaaaccga gaccagcggc atcgtgctgg aggcggtgat ctcgccgcag cttgatcgat
      961 cgcgttatta ttttacggcc ctttacaaca gcatagacca ggaacagggt atcggcaccc
     1021 tgagcacgaa cagctccctt tacgaaaccg ccacacttgg cgcgacctat cttgtaacac
     1081 gcaatctgcg gctgatggcc gagttcaccc ggaatctgga aatggatgcc aaccggatgc
     1141 tggtcggcct ggtgagcgga ttctagctgc gaagcggatt tggcgcagtc caaatcaggt
     1201 atagatcccg gcgtggcagt gccggcgctg ctacagcgta aaaggaggag aaatcctcct
     1261 tttttttatg ccggattggg atcctggaag gtttcgtcgc gggagagccg ctgacgcatc
     1321 acctcggcca gaaggatggc cgaagcgtgg ctcagggaga gtgaaccggc tgcggagagc
     1381 atggggattt taataaaacg gtcgcagtag ccgcgcaccg ctgcggaaag gccgcgtttc
     1441 tcgcccccca gggcgatgag caccgaccgg gtgagatcga cctgatagat cgtgcgctgg
     1501 gcgttggcga tgcagccgta aatccgcacc ccgagagacg atatccgggg cagaagggat
     1561 tcggcctggt cgaagacaca gagagggagg cgttcaaaag cccctgaaga ggcacgggaa
     1621 acggcagcgc cgtcaaagtc ccagaggtgc tttttcagca gcaccgcatc gacgccgcag
     1681 gcttcagcgg tgcgcaggac aaagccgagg ttctggctgt catcgatccc ctccagaagc
     1741 agaagaaaga ggggaaatcc gcagctgttg aggtgtttga agagctggtc aatggcgaga
     1801 tggggacgag gcgagcagag ggcgaggacg ccgccatggg tgtgaccatg ggccatggca
     1861 tcgagctcat ccggactgac cagtttgacc gggatctgcc gggcttgtgc cagttcaatc
     1921 aggggtgcgc tggtctccgc atgcagggaa ttgcgcagaa ccagaaggtg tattctgcgc
     1981 tggcgggcac gcagggccgc ttcaacgcat atttttcctt ccagctgttc catcctcagg
     2041 cctccccgag agcggcgagg tcatcctggc tccactccgg catggcgccc tgccgggtag
     2101 cgacaaatgc ccccaacctg ttggcaaatt cagccatctc ttcaagtgat gctttattca
     2161 aaatgctcca gatcaggccg gcagcaaagg catctccgca gccggtagtg tccactgcgc
     2221 gcacccgaaa tccgggatgg tgaaggtttt gatcaggtgt gagcagccaa catccccgac
     2281 ttcccagggt gacggcagcc attttgatat tgtaggagtg caggaggctg agcaaaaagt
     2341 cctgcctgtc catcttgccg gcatcaaggg cctgctggag gatggcgagt tccgtctcat
     2401 tcatcttgac gatatcgcac aggctgaggg aattcagaac gatgtcacgc gtggagtcat
     2461 tccagccgcg cagattgaga tcgaagagaa ggagagcctt gtccgcatgg tgcaggcagg
     2521 cgcggatggc gttacgcgag gtttcctcgc gctgggcgag gactccatag agcagggcat
     2581 cgaggctggg taaaagcgtg cgccagacct ggtcgcattc cagctgatcg aaggcagtat
     2641 cgcggctgca ctcgaagcgt ggcgccccgg ccggaccgag gtggacacgc acactgcctg
     2701 tgggcttgtc cggactggtc tggatggcgg agacgtcgac gccgtttctg gccaggaggg
     2761 ccttgaggtc gcggccggcc tggtcattcc ccacacggct gagcaggaga gcgtcggctc
     2821 cgcagcggcg ggcatgcaaa gcaaaattgg ccggcgctcc accggcatga cgacgatcat
     2881 cgtaaatatc ccaaagcagc tcgcctaatc cggcgatttt gtgtgggatg gtcattcttc
     2941 agcctgcagg gctactgtca gagaggggcc gccaggcatg cgaccggaac ctgtgcgcgg
     3001 atcgatagtg gcaaaggtga tgcggtcggg gacctgaaag ttggtgatct gtgcgctggc
     3061 gaaggcgttt ttcataaaca gggcccagat aggcagagca gccctgctgc cggtgatccc
     3121 cttgccgcgg ctgtcgcgca tggaacggtt gtcgtcgtaa cccacccaga cggcgacggc
     3181 gagctccggc gtaaagccga caaaccaggc gtcgcggtag tcattggagg tgccggtctt
     3241 gccggcgcag gggcgataga aaccatagct gcgcacggcg ctgccggttc cccccttctc
     3301 gacgacgccg gtgagcatgt cgagcaggag gaaagcagtc tgggcatcgg tcacgcgctg
     3361 gctcttcgga atggtttcct ccaccacttc attcagctcg ttatgtatgg a
//
LOCUS       DAOPWU010000360         3374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_28705_length_3374_cov_3.471829, whole genome shotgun sequence.
ACCESSION   DAOPWU010000360 DAOPWU010000000
VERSION     DAOPWU010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28705_length_3374_cov_3.471829"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2667
                     /locus_tag="PKM23_13660"
     CDS             <1..2667
                     /locus_tag="PKM23_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNY92564.1"
                     /translation="WQTISFFTLAGDKRSLVIRLPLQRHSPALFQTTHLTSSWYVAEH
                     GLAIADFNGDDTEDIFAVATGAANHLYLLNLLQGGQMETESAAAAGLAGVAVLPTGIV
                     NYDEGASAADVDNDGDQDILVTSLYGHNLLYRQIRRGRYREESEFAGIDNCLARTCSA
                     VWADANSDGAIDLYLSNEDSTNRLYLNNGAGIFRDVTCEAGLLIRRGGGGSAFGDIDD
                     DGDPDLFVPRYGLANLLFRNEGAPRPGNVPLFRECGQEAGVAGIDTLARSTSASWGDV
                     DNDGDLDLFVTNLVHSNQLLLNDGRGRFEETTAARGVTSRDLSQTALLFDADNDGDLD
                     LLTGSRALNRFHANKGKGYFYEATEEAGITRLTNAGGMAAFDLGEDGDLDVYLGQGEQ
                     HSALYDNHSNNRHWLEVKLRGDRGNRDAIGARLWLYRDGHLGEPAHLLGMREINGGSG
                     YNSMNSRIAHFGLPDDGRFALRVRFPGGRIREITGISGGRLLEVQESEGWSRQRALFA
                     KWAKRLRANPEPRLPLTLIALFSSLLILFEFYLLRYKKWPVHTVWLMVLLPALLLLLL
                     LIVLVAMPVWLHYGLSLALPAIAWALAFGLYTSRQDTNDHAIADFEKLYLAVSAFFHG
                     EWASRKLNRIEFFVQNLEEGRKPAPEVAEAIAGAINEYFTMIVPELRRILELASGSRI
                     PKSLHTSLEVAMLNLTAALSELKVELHLGKSAARQNALRCSQILQMEMQSLRKEVGRH
                     FSCRVDQLLEESLRQNRRPGVDYHLTCTTSEPLWARIPGAALSQILENLLDNAHAAIS
                     ASPNKEITIALEGNADQIRIRVSDSGPGVRTDIARRLFHERVSSKSSPGGFGLYHARE
                     VLTKFAGTVRLLEQEEGTGARFEITLKRIEHE"
     gene            2660..>3374
                     /locus_tag="PKM23_13665"
     CDS             2660..>3374
                     /locus_tag="PKM23_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNY92565.1"
                     /translation="MSDRLRLLLIDNDAQFAADFALLGRESFVIKHVISGEEGVEALK
                     HEEPDAVLLDLRMGEGWDGLQTLQRIREEHPDLPVVMVTDHAGVETAVQAMKLGALHY
                     TSKKPNMGTLYTVIQRELRQANLRRLYLEENSKHYGKLIGDSPEMHRVYRQIARIAAA
                     PSHILIQGECGTGKEVAAHEIHARSSRAGQPFVAVDSGAIAPGLFESELFGHEKGSFT
                     SADSLHRGRIEMAGEGTLFL"
BASE COUNT          650 a         1167 c          951 g          606 t
ORIGIN      
        1 tggcagacca tctccttttt caccctcgcc ggcgacaaac gcagcctggt catccgtttg
       61 cctctgcagc gccactctcc tgctcttttt cagacaaccc atcttaccag cagctggtac
      121 gtggccgaac acggcctggc gatcgctgat ttcaacgggg atgacacgga agatattttt
      181 gccgtggcga caggggccgc caatcatctc tatctgctca acctgctcca gggcggccag
      241 atggagaccg agagtgccgc agccgcaggc ctggccggcg tggccgtcct gccgaccgga
      301 atcgtcaact atgacgaagg ggcttcggcg gccgacgtcg ataatgacgg ggatcaggat
      361 atcctcgtca cctccctcta cggccataat ctcctctacc gccagatccg ccgcggccgc
      421 taccgggaag aatcggaatt cgccggcatc gacaactgcc tggcgcgcac ctgctccgct
      481 gtctgggccg acgccaacag cgatggggcc atcgatctct atctctccaa cgaggattcg
      541 accaaccggc tctatctcaa caacggtgcc ggcatcttcc gcgatgtcac ctgcgaggcc
      601 ggactgctca tccgccgcgg cggcggcggt tccgccttcg gcgatatcga tgacgacggc
      661 gatcccgatc tcttcgtccc gcgctacggc ctggccaacc tcctcttccg caacgaaggc
      721 gcaccccgtc ccggcaacgt cccccttttc cgggaatgcg gacaggaggc gggggtcgcc
      781 ggcatcgaca ccctcgcccg ctccaccagc gccagctggg gcgacgtcga caacgacggc
      841 gacctcgacc tctttgtcac caatctggtt cactccaacc agctccttct caacgacggc
      901 cgcggccgct tcgaggagac cacagcggca cggggagtaa ccagccgcga tctcagccaa
      961 accgcactcc tttttgatgc ggacaacgat ggcgacctcg acctcctgac cggcagccgc
     1021 gcgctcaacc gcttccatgc caacaaaggt aagggttatt tttacgaggc cacagaggag
     1081 gcgggcatta ctcgcctgac caatgcaggc ggcatggcgg ccttcgacct gggtgaggac
     1141 ggcgaccttg atgtctacct cggtcaaggg gagcagcaca gcgccctgta cgacaaccac
     1201 tcgaataacc gccactggct cgaggtcaaa ctccgcggcg accgcggcaa ccgtgacgcc
     1261 atcggcgccc gcctctggct ctaccgcgac ggccacctcg gcgaaccggc gcatctgctc
     1321 ggcatgcgcg agatcaacgg cggcagcggc tacaactcga tgaacagccg catcgcccat
     1381 ttcggcctgc ccgacgacgg ccggttcgcc ctgagggtgc gctttcccgg cggccggatc
     1441 cgcgaaatta ccggcatcag cggcggtcgc ctgctcgagg tgcaggagtc agagggctgg
     1501 tcacggcagc gcgccctgtt cgcgaaatgg gcaaagcgcc tccgcgccaa tcccgagccc
     1561 aggctgccgc tcactctcat cgcccttttc tcctctctgc tgatcctctt cgagttctac
     1621 ttgctcaggt acaaaaaatg gcccgtgcat accgtatggc tgatggtgct gctccccgcc
     1681 ctgctcctcc ttctgctgct cattgtgctc gttgccatgc cggtatggct gcactacggt
     1741 ttgagtcttg cactgcccgc catcgcctgg gccctcgctt tcgggctcta cacctcccgc
     1801 caggatacca atgaccatgc gatcgcggat ttcgagaaac tctaccttgc ggtttccgcg
     1861 ttctttcacg gcgaatgggc gtcgcgcaaa ctcaaccgca tcgagttctt tgttcagaac
     1921 ctggaggaag ggcggaagcc tgcccccgag gtggccgagg ccattgcagg cgccatcaat
     1981 gaatacttta ctatgattgt gcccgagctg agaaggatcc tcgaacttgc ctccgggagc
     2041 cggatcccca aatctcttca cacctccctc gaagtggcga tgctcaacct caccgccgct
     2101 ctcagcgaac tcaaggtcga actccatctc ggcaaaagcg cagcccggca gaacgccctg
     2161 cgctgcagcc agattctgca gatggaaatg caaagtctgc gcaaggaggt cggccgccat
     2221 ttttcgtgcc gggtagacca gctcctcgag gagagcctgc ggcagaaccg ccgtcccggc
     2281 gtggattatc acctgacctg tacaaccagc gagcccctct gggcgcgcat ccccggcgcc
     2341 gccctttcgc aaatcctgga aaatctcctc gacaacgccc acgccgccat cagtgcctcg
     2401 cccaacaagg aaatcaccat cgccctggag ggcaatgccg atcagatccg catccgcgtc
     2461 agcgacagcg gccccggtgt gcgcactgac atcgccaggc gcctctttca tgagcgggtg
     2521 agcagcaaat ccagccccgg cggcttcggc ctatatcatg cacgggaagt tctgaccaaa
     2581 ttcgccggca ccgtccgcct cctcgagcag gaggagggca caggcgcccg cttcgaaatc
     2641 accctcaaga ggatcgaaca tgagtgaccg gctgcgtctt ctgctgatcg ataatgatgc
     2701 ccagtttgcc gctgatttcg ccctgctcgg cagggagagc tttgtcatca agcacgtaat
     2761 aagcggcgag gagggagttg aagcgctgaa gcatgaggaa cccgatgctg tcctgctcga
     2821 cctgcgcatg ggcgaggggt gggatggact gcagaccctg cagaggatac gggaagagca
     2881 tcccgacctg ccggtagtga tggtcaccga tcatgccggt gtggagaccg ccgtacaggc
     2941 gatgaagttg ggcgccctcc attacacctc caagaaaccc aatatgggta ccctctacac
     3001 ggtcatccag cgcgaactgc gccaggcgaa tctgcgccga ctctacctgg aggagaacag
     3061 caaacattat ggcaaactga tcggcgactc ccccgagatg caccgggtct accgacagat
     3121 cgcccgcatc gccgccgctc cctcccacat cctcattcag ggcgaatgcg gcaccggcaa
     3181 ggaggtagcg gcgcacgaga ttcacgcccg cagcagccgc gccggacagc ccttcgtcgc
     3241 cgtcgacagc ggggccatcg cgccgggcct cttcgagagt gaactcttcg gccatgaaaa
     3301 gggctccttc accagcgccg attcccttca ccgcggccgc atcgaaatgg ccggcgaggg
     3361 caccctcttc ctcg
//
LOCUS       DAOPWU010000361         3353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_28951_length_3353_cov_4.233475, whole genome shotgun sequence.
ACCESSION   DAOPWU010000361 DAOPWU010000000
VERSION     DAOPWU010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28951_length_3353_cov_4.233475"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1432)
                     /locus_tag="PKM23_13670"
     CDS             complement(113..1432)
                     /locus_tag="PKM23_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY92566.1"
                     /translation="MEQTRRNFLRLAALGTAGATMGMSARSYSRIAGANERINFAIIG
                     LHSRGYAHLDGIRFAPNTHISHVCDVDRHELEKFVLVVEQSTGRSTVGVRDFRTILDD
                     KSVDAVTIATPEHWQAPMAILALKAGKHVYVEKPGSHNPHEGELLVQVQQRYGKLVQL
                     GNQQRSSPHSREIIQKIHDGLIGTPYFGKAWYTNTRQSIGKGREVPVPEWLDWELWQG
                     PAPRRPYKDNIHPYNWHWFWHWGTGETLNNGTHEVDLCRWALQLGYPERISAAGGRYH
                     YRDDWEFYDTLVANFEYKDKLITWECLSCQGKQPYGRGRGVTIHGTAGTVLLDRDGYE
                     LYDLNDKKMAEFKIHSENTTLDLQSRDAMTNGHFVNFIDGIRLGTPLNSAISLCNPSV
                     AMLQIANIAWYVKKTLELDPQNGHIRTEGRARSMWRRKYEKGWEPVV"
     gene            complement(1538..>3353)
                     /locus_tag="PKM23_13675"
     CDS             complement(1538..>3353)
                     /locus_tag="PKM23_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNY92567.1"
                     /translation="GREVIQLNPDVARRYFNPKKVVVPNLYPETFSRTRTENTFRIFC
                     LGGSTTAGFPFEFQVPFPARLKNLLASQYPRRRFEVINLGLSAVNSFTVLDFIPEVLE
                     QKPDLILLYMGHNEFYGAYGSASAVTAGRSGALIRFYLGLQRLRLVQMVRAAVIAATP
                     AGQQDPENSTLMEQIAAGGSIAFRSAQYKLTLENYRRNLELILRKCRRAGVPVIAGTL
                     VSNLKDQPPLGSISRGESDPALSHKIGELAAGCDGLLEQEEFIRALVVAHELIALDSL
                     NASHWYRAGRIQLALGDSLAALESLTEAKDRDPVRFRASEDFNHVLTAVARADGAGLA
                     DIRQLFALASPGGIPGAELICDHLHPNPGGYLLMARGFAREIGKSSLVQTRPAGDGIP
                     ENPVAVTDLDWDIGLLRVYKLKHRWPFADRKVDWSLYKPHGETAAAGVALDYLFNHHN
                     WVQAHYAMADTLSRRGEHEKARGEYQAVLACYPEQPQPLIKMAQSYEADAEWGEAEQA
                     LQSALQLTEKKGMIYYRLALVQSRQKKLAAAIQNIQHAIISPELSPEQRTAAKYDLAL
                     FFIAVKRMEYAGEVLADILGEYPGYGPALDLKRKYFDN"
BASE COUNT          638 a          964 c         1057 g          694 t
ORIGIN      
        1 gggcacgaaa agtggtctta cgagcagaca tgaccatatc ctccaggtat aatacacact
       61 ccggggcacg ccagcggctc cccggcaggg attatacctc atctacggaa aatcagacca
      121 caggttccca tcctttttcg tattttcgcc gccacatcga acgcgccctg ccttccgtgc
      181 ggatatggcc gttttgggga tcgagctcga gggtcttctt gacataccag gcgatattgg
      241 cgatctgcag catggcgacg ctcgggttgc agagggagat ggccgaattc aggggggtac
      301 ccaggcggat gccgtcgata aagttgacaa agtggccgtt ggtcattgcg tcccggctct
      361 ggagatccag ggtggtgttt tcagagtgga ttttgaactc ggccatcttt ttgtcattga
      421 gatcatagag ctcgtagccg tcgcggtcga ggagtaccgt cccggccgtg ccatggatgg
      481 tgacgccgcg gccgcggcca tagggctgct tgccctggca gctcaggcac tcccaggtga
      541 tcagcttgtc cttgtattca aagttggcca ccagggtatc gtaaaactcc cagtcatccc
      601 gatagtgata acgtccccct gcggccgaga tgcgttccgg atagccgagc tggagggccc
      661 agcggcagag atcgacctca tgagtgccgt tgttgagggt ttccccggtg ccccaatgcc
      721 agaaccagtg ccagttgtag ggatggatat tgtccttgta gggccggcgc ggggcaggcc
      781 cctgccaaag ctcccaatcc agccattcgg gcaccggcac ctccctgccc ttgccgatcg
      841 actgccgggt gttggtatac caggccttgc cgaagtaggg ggttccgatc aggccgtcat
      901 gaatcttctg gatgatctcg cggctgtgcg ggctggagcg ctgctggttg cccaactgca
      961 ccagtttccc gtacctctgc tggacctgca ccaaaagctc accctcatgg ggattatggc
     1021 tgccgggttt ttccacatag acatgtttgc ccgccttgag ggccaggatg gccatcggcg
     1081 cctgccaatg ctccggagtg gcgatggtga cggcatcgac tgacttgtca tccaggatag
     1141 tgcggaaatc cctgaccccg accgtgcttc tgccggtgga ctgctccacg accaggacga
     1201 atttctcaag ctcgtggcgg tcgacgtcgc agacatggct gatgtgtgtg ttcggagcga
     1261 agcggatgcc gtccagatgg gcataaccac ggctgtgcag cccgatgatg gcgaaattaa
     1321 tgcgttcatt ggctccggcg atgcggctgt agctccgggc ggacatcccc atggtggcgc
     1381 cggcggtgcc aagagcggcc agcctgagaa aatttctgcg ggtctgttcc atatcacctc
     1441 tattcgctga tgaattgact cccgggctgg caagtcgttt aatccctggc ctgagcagac
     1501 cgggcaacag gccccggctg cagcctgatg ggcacagtca gttgtcgaaa tatttccgtt
     1561 ttaaatccag ggcaggcccg tagcccggat actccccaag aatgtccgcg agcacctccc
     1621 cggcatactc catcctcttc acggcgataa aaaagagggc aagatcatat ttggctgctg
     1681 tacgctgttc cggacttagt tccggggaga tgatggcatg ctggatgttc tggatcgccg
     1741 cggccagctt tttctgccgt gactggacca gcgccagcct gtaataaatc atccccttct
     1801 tctccgtcag ctgcagggct gactgcaagg cttgttcagc ctccccccac tccgcatcgg
     1861 cctcataact ttgcgccatc ttgatgaggg gttggggctg ttccgggtag caggcgagca
     1921 ctgcctgata ctcacctcgt gccttttcat gctccccgcg gcgggaaagg gtatcggcca
     1981 tggcataatg cgcctgcacc cagttgtggt gattgaagag ataatcgagg gcgacgccgg
     2041 cggctgcggt ttcaccgtgg ggtttgtaga gggaccaatc gaccttgcgg tcggcgaagg
     2101 gccagcggtg ctttagcttg taaacacgca gcaggccgat gtcccagtca agatcggtca
     2161 cggcgacggg attttccggg attccgtccc cagccggccg ggtctggacg aggctgctct
     2221 tgccgatctc gcgggcgaaa ccccgcgcca taagcaggta tcctcccgga ttgggatgga
     2281 gatgatcgca aatcagctcc gcaccaggga tcccgccagg cgaggccagg gcaaagagct
     2341 gccggatatc ggcgagcccg gcgccgtccg ccctggcgac tgcggtgaga acatggttga
     2401 aatcctcgct ggcgcgaaaa cgaacgggat cccgatcctt tgcctcggtc aggctctcca
     2461 aagctgcgag ggaatcgccg agggcgagct ggatcctgcc ggcgcggtac cagtgactgg
     2521 cgttgaggga atccagggcg atgagttcgt gggccaccac cagggctcga atgaactctt
     2581 cctgctcaag gagaccgtcg cagcctgcgg ccagctctcc gattttatgg ctgagggcgg
     2641 gatcgctttc accgcggctg atgctgccca ggggcggctg gtccttgaga ttggagacca
     2701 gggtgccggc gatgacgggc acccccgcac ggcggcattt gcgcaggatg agttcgagat
     2761 tgcgacgata gttttccagg gttaatttgt attgggccga gcggaaggca atgctccctc
     2821 cggcggcaat ttgctccatc agggtgctgt tttccggatc ctgctgcccg gctggcgtgg
     2881 cggcgatcac agccgcgcgc accatttgca ccagcctcag ccgctgcagc cccagataga
     2941 agcggatgag ggcgccgctg cggccggccg tgacggccga ggcgctgcca taggcgccat
     3001 agaactcatt atggcccatg tagagcagga tgaggtcggg cttttgctcg agcacctcag
     3061 ggatgaaatc gagcacagtg aaactgttga ccgccgaaag accgagattg atgacctcga
     3121 agcggcgccg gggatactgg ctggcgagca ggttcttgag ccgggcggga aaagggacct
     3181 ggaactcgaa gggaaaaccg gcggtggtgg agccgcccag acagaagata cggaaggtgt
     3241 tctccgtccg ggtgcgggaa aaagtctcgg gataaaggtt gggcaccacc acctttttgg
     3301 gattgaaata gcgtctggct acatcgggat tgagctgaat gacctccctc ccc
//
LOCUS       DAOPWU010000362         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_29168_length_3336_cov_4.201158, whole genome shotgun sequence.
ACCESSION   DAOPWU010000362 DAOPWU010000000
VERSION     DAOPWU010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29168_length_3336_cov_4.201158"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2233
                     /locus_tag="PKM23_13680"
     CDS             <1..2233
                     /locus_tag="PKM23_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNY92568.1"
                     /translation="EVTANLVRNQLGIAGVYLSWALIHGTIGFPVIVLPFLLIALGVF
                     KLLGRETAQLRRWSWFTFLLALFISLGFAIYHYVIRAPLAQAYEYSGFIGVLLATLLR
                     KLIGIYGAVIVLVVLTVINIVTFTSISLREIHANLSAGIARLGVRIGQNIREMRGVKS
                     KPEAAVKRFRAADSKGPIVARPLPIDLAEEEEIAPPPVKVALPPRPAPVRPAAQEWEG
                     EEELTEEEEGELVPSLKPSGPYTFPPLSLLQLPEEAIEPASEEELRANARMLEEKLAD
                     FDIQARVTEIHPGPIITRYEMELAPGIKVSRITGLADDLAMAMRAKRIRIVAPIPGKN
                     AIGVEIPNEKPQMVYLREILASSEFQSARSVLSIGLGKTISGAPYVTDLASMPHLLVA
                     GSTGSGKSVCLNTIIASFLYKAHPAQVQLVLIDPKRLELSTYSRLHPYHLSYVEGVRE
                     KVATSAKSAIAVLKSAEQEMERRYDMLATAGVRNIEEYNARAAEGRIHTESGAVASAL
                     HYLVIVVDELADLMLTSSASKDVEEPIARLTQMSRAVGIHLIVATQRPSVDVITGVIK
                     ANFPARMAFQVASKVDSRTILDMNGAEKLLGKGDMLFLPPGSAEPIRIHNAYIGLEEI
                     ESVIAHIHTQPPIVKSPLPACIDDEMGGGSDERGPGNGRRDPLFSEAMRIVVRTGQGS
                     VSILQRRLKVGYSRAARLIDELEMAGVVGPFEGSKAREVLLRPEDLENLPGDSENDDE
                     EEY"
     gene            2309..2941
                     /locus_tag="PKM23_13685"
     CDS             2309..2941
                     /locus_tag="PKM23_13685"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNY92569.1"
                     /translation="MKRLFTLLIIFGLAASLSAQEAADLLGKIQKRYEAMKSVCADFT
                     QTFNWELAGERQVFEGKICTRNGVQFRIETGDQLIVTDGKTVWTMPHGNKQVIIDDAS
                     ENGDTNPFLKSFLKNFVDNYASELLGSESIAGAPCHHIRLRAKSGDEFTQEVEIWADQ
                     KSLLMNRIRQVDLNGNSSTYEVRNIDLNARLTDATFRPEIPAGYEVVDMR"
     gene            2941..>3336
                     /locus_tag="PKM23_13690"
     CDS             2941..>3336
                     /locus_tag="PKM23_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNY92570.1"
                     /translation="MPRSLTRTLLLAVLLAPAAFAQQPAQSEIPHAAQYFLGSQDQVL
                     MAVNVWGFVHKPGQYMVPYETDLISLLSFAGGPREEARIKSIKLVRPAAGESKTEVIE
                     IDVKAYLDKGRIEEIPILRPGDTVVVSGTS"
BASE COUNT          624 a         1157 c          975 g          580 t
ORIGIN      
        1 ggaggtcact gccaatctgg tccggaatca gctcggcatc gccggcgtct atctctcctg
       61 ggccctcatc cacggcacga tcggctttcc cgttatcgtg ctgccctttc tcctcatcgc
      121 cctcggggtc ttcaagctcc tcggccggga gaccgcgcag ctgcgccgct ggagctggtt
      181 cacctttctc ctcgcccttt tcatctcgct cggcttcgcc atttatcact atgtcatccg
      241 tgcccccctc gcccaggcct atgaatattc gggctttatc ggggtgctgc tggcaaccct
      301 gctgcgcaag ctgatcggga tctacggggc ggtcatcgtc ctggtcgtgc tgacggtgat
      361 caatatcgtc accttcacct cgatctccct gcgcgagatc catgccaatc tctccgccgg
      421 catcgcccgg ctcggggtgc gcatcggcca gaacatccgc gagatgcgcg gggtcaagag
      481 caagccggag gcggcggtga agcgcttccg cgccgctgat tccaaaggcc cgatcgtcgc
      541 acggcctctg cccatcgatc tcgccgagga ggaggagatt gcgccaccgc cggtcaaggt
      601 agcgctgccc ccgcggccgg cgccggtgcg cccggcagcc caagagtggg agggggagga
      661 ggagctgaca gaggaggaag agggcgagct ggtgccttcc ctcaagccga gcggtcccta
      721 tacctttccg cccctgagcc tgctccaact gcccgaggag gcgattgaac cggcctccga
      781 ggaggagctg cgcgccaacg ccagaatgct cgaggaaaag ctcgccgatt tcgacatcca
      841 ggcacgggtc accgagatcc atcccggccc gatcatcacc cgctatgaga tggaactggc
      901 gcccggcatc aaggtcagcc gcatcaccgg actggccgat gacctcgcca tggcgatgcg
      961 cgccaaacgc atccgcatcg tcgcccccat ccccggcaag aacgccatcg gcgtcgagat
     1021 ccccaatgaa aagccgcaga tggtctatct gcgcgagatc cttgcctcca gcgagttcca
     1081 gtccgccaga tcggtgctca gcatcggcct gggcaagacc atctccggcg cgccctacgt
     1141 caccgatctg gcctcgatgc cccatctcct cgtcgccggc tcgaccggct ccggcaagag
     1201 cgtctgcctg aataccatca tcgccagctt tctctacaag gcccacccgg cccaggtcca
     1261 gctcgtcctc atcgacccca agcgcctcga gctctccacc tacagccgcc tccatcccta
     1321 ccacctctcc tatgtggagg gggtgcgcga gaaggtggct accagcgcca agagcgccat
     1381 cgcggtgctc aagtcggccg agcaggagat ggagcggcgc tacgacatgc tcgccaccgc
     1441 cggagtgcgc aatatcgagg agtacaacgc gcgcgcggcc gaggggcgca tccataccga
     1501 gagcggcgct gtcgcctcgg ccctgcacta tctggtcatc gtcgtcgacg aactcgccga
     1561 cctcatgctc acctccagcg cctccaagga tgtggaggag ccgatcgccc gcctcaccca
     1621 gatgtcccgc gccgtcggca tccatctcat cgtcgccacc cagcgtccct cggtggacgt
     1681 catcaccggg gtgatcaagg ccaatttccc ggcgcgcatg gctttccagg tggccagcaa
     1741 ggtcgactcc cgcaccatcc ttgacatgaa cggggcggaa aagctcctcg gcaagggcga
     1801 tatgcttttc ctgccgcccg gcagcgccga gcccatccgc atccacaacg cctatatcgg
     1861 actggaagag atcgagagcg tcatcgccca tatccatact cagcccccca tcgtcaagtc
     1921 gcccctgccg gcctgcatcg acgacgagat gggcggaggc agcgatgagc gcggcccggg
     1981 caacggccgg cgcgatcccc tcttcagcga ggcgatgcgc atcgtcgtcc gcaccggcca
     2041 gggctcggtc tccattctgc agcgccgcct caaggtcggc tactcgcgcg cggcccggct
     2101 catcgacgaa ctcgaaatgg ccggtgtggt cggccccttc gagggcagca aggcccgcga
     2161 ggtcctgctg cggccggagg acctcgaaaa cctgcccggc gacagcgaaa atgacgatga
     2221 ggaagagtat tgaccaccgg gcgggaacac cgcgcccgct ctgacgttca agatggggat
     2281 gtaacgtcac atttataaag gattcgccat gaaacgactt ttcaccctcc tgatcatctt
     2341 cggcctggcc gcttctctct ccgcccagga ggccgccgac ctcctcggca aaatccagaa
     2401 gcgctatgag gccatgaaga gcgtctgcgc cgatttcacc cagaccttca actgggagct
     2461 ggccggcgag cgccaggttt tcgagggcaa gatctgtacc cgcaacggcg tccagttcag
     2521 gatcgagacc ggcgaccagc tcatcgtcac cgacggcaag accgtctgga ccatgccgca
     2581 cggcaacaag caggtgatca tcgatgacgc ctccgagaac ggcgatacca atccctttct
     2641 caagtccttt ctcaagaatt tcgtcgataa ttatgccagc gagctcctcg gcagcgagag
     2701 catagccggc gcgccctgcc atcacatccg cctcagggcc aagagcggcg acgagttcac
     2761 ccaggaggtc gagatctggg ctgatcagaa gagtttgctc atgaaccgga tcaggcaggt
     2821 tgatctcaac ggcaacagct ccacctatga ggtgcgcaac atcgatctca acgcccggtt
     2881 gaccgacgcc actttccggc cggagatccc cgccggatac gaagtcgtgg acatgcgctg
     2941 atgcctcgat ccctgacgcg gacactcctg ctggccgtgc tcctggcacc ggcagccttt
     3001 gcccagcagc ctgcccagtc cgagatcccg cacgcagcgc agtattttct cggcagtcag
     3061 gatcaggtgc tgatggcggt caacgtctgg ggctttgtgc acaagccggg acagtacatg
     3121 gtcccctatg agaccgattt gatctccctg ctctccttcg ccggcgggcc gcgcgaggag
     3181 gcgcgcatca aaagcatcaa actggtgcgt ccggccgccg gcgagagcaa gacggaggtc
     3241 atcgaaatcg acgtcaaggc ctatctggac aagggacgga tcgaggagat tcccatcctg
     3301 cgcccgggcg atacggtggt ggtctcgggc acctcg
//
LOCUS       DAOPWU010000363         3328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_29262_length_3328_cov_3.219371, whole genome shotgun sequence.
ACCESSION   DAOPWU010000363 DAOPWU010000000
VERSION     DAOPWU010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29262_length_3328_cov_3.219371"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1635
                     /locus_tag="PKM23_13695"
     CDS             259..1635
                     /locus_tag="PKM23_13695"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNY92571.1"
                     /translation="MNKKSNPKKIQIINGNIILPAAVFSGNLEIEEGRITYIGPGCRM
                     TNPDCQINAIGRYILPGFIDIHTNGIAGFDLTNGVYDLESGRFRNDEATYIKGLEWAL
                     YRYAKTGVTRAILTSLAAPLGQLKRIFSYVNKYKSEGAQTPWKDILGGLFVEGTFMKL
                     VSFRGAHNPGYFKKPSVELFDDLQKMSGGMIKVVNVVPEWDQSALQLIRYLAARNVIC
                     AAGHTGATGRQYELAIQNGLRLAVHFLNGPTGSSSKSLDGGGAVETVLRSDSMFVELI
                     VDGYHVDKAYVLDTIKRKNYDRVIAITDSMFAAELPGLKQFKIFNITGKVAENGEYLQ
                     IADREDALFGSKLVMVEAFNNLLNWLTAPTGGIWNVLHEPLDFNQALVKVSSMCSDNP
                     ARVLGIYNSQDNTGSIEIGKRADLLIGEIARVGEKYRFNINQVMVNGMTIDEFTGQGI
                     TEPVAVLA"
     gene            1752..2456
                     /locus_tag="PKM23_13700"
     CDS             1752..2456
                     /locus_tag="PKM23_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010272187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNY92572.1"
                     /translation="MGQAAAAGVADKIRALAARQDAIRMVFAAAPSQNEFLDALIKTE
                     GLPWAKITAFHMDEYIGLDPAAPQRFGQFLRARLFDRVPFGRIHYLNPSTERVAQECE
                     RYTALFTAAPIDIICMGIGENGHIAFNDPPVADFNDPRIVKVVDLDPVCRQQQVNDGC
                     FASLNEVPKQAITLTVPALAAGKSLFIIVPGKTKSKAVHDTLLGEIGTHCPASILRNH
                     PDAELYLDADSAAALA"
     gene            2495..>3328
                     /locus_tag="PKM23_13705"
     CDS             2495..>3328
                     /locus_tag="PKM23_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNY92573.1"
                     /translation="MRNNTVKLLSTLLTLMFVHLAAGQPPASQLNLMPVPEKVELLAG
                     KYRLDAGFTVRIAGAQSGRLVKGASRILDRLTGRTGLFLANPTSLLDASAPAAGMLLT
                     AGRQGELAPLQDESYDLVITPDGIKMTAETDIGIIRGLETVLQLLDADENGYFFPAVR
                     VHDKPRFPWRGLLIDAGRHFIPADIIRRNLDAMAAVKMNVLHWHLTEDQGFRIESKAF
                     PKLHQLGSDGLYYTQAEIAGIVAYAGDRGIRVMPEFDLPGHATSWFVGYPELASAPGP
                     YT"
BASE COUNT          753 a          957 c          924 g          694 t
ORIGIN      
        1 cccccttcgc cctctataca ggactcgaac tgctggcctc gttcatcgtc ttttacggcg
       61 aaggctttat cagcaagcat accccggaag aaaaaaagcg catcgacgga ctctttgcgc
      121 gtctgaaaaa atagccgggg gagagaaata taaaaaagcc ggaaataagc gaggccgaat
      181 ctattcacgc cgggctctca ccggggattc gggttcatat cgctgggatg attggtcttt
      241 ctgataacgg ggcggtcaat gaacaaaaag tctaatccaa aaaagattca gatcatcaac
      301 ggtaacatca tattgcctgc ggcggttttc agcggcaatc tggaaataga ggagggccgc
      361 atcacctata tcgggcccgg ctgccgcatg actaatccgg attgtcagat caatgcgata
      421 gggcgataca tcctgccggg ttttatcgac atccatacca acggcattgc cggctttgat
      481 ttgaccaacg gcgtctatga tctcgaatca ggacgttttc gcaatgatga agcgacctat
      541 atcaaaggcc tcgagtgggc attgtaccgc tatgccaaga ccggcgtcac ccgcgccata
      601 ttgaccagcc tcgcggcgcc tctcgggcaa ttgaagagaa ttttcagcta tgtgaacaaa
      661 tacaagagcg agggagcgca aacaccctgg aaggacatcc tgggcggtct gttcgtcgaa
      721 ggcacattta tgaagcttgt cagttttcgc ggcgcccata atccggggta ctttaaaaaa
      781 ccctctgtcg agctattcga tgacctgcag aaaatgagcg gagggatgat caaggtggtc
      841 aatgttgtcc cggagtggga tcaatcggcc ctgcagctga tccgctatct ggccgcccgg
      901 aatgtcatct gcgccgcagg ccataccggc gccaccggcc ggcaatatga attggccatt
      961 cagaacggtc tgcggctggc ggtccacttt ttaaacggac ccaccggttc ttccagcaag
     1021 tcactcgatg gcggcggagc cgtagagacg gtcctgcgtt cggacagcat gttcgtggaa
     1081 ctgatcgtcg atggatacca tgtcgacaag gcctatgtgc tggatacgat caaacgcaag
     1141 aactatgacc gggtcatcgc catcacggac agcatgttcg ctgcggaact gccgggattg
     1201 aaacagttca agatcttcaa catcaccggc aaagtcgccg agaacggtga atacctccag
     1261 attgccgacc gggaggatgc cctgttcggc agcaagctgg tgatggtcga ggctttcaat
     1321 aatctgctca actggcttac ggcccccacc ggcggcatct ggaatgttct gcatgagccg
     1381 ctcgatttta atcaggcact ggtcaaggtc tcttcgatgt gcagcgacaa tcccgccagg
     1441 gtcctcggaa tttacaattc ccaggataat accggcagca tcgagatcgg caagcgggcc
     1501 gaccttctca tcggcgagat cgcccgggtg ggagaaaaat accgtttcaa catcaatcag
     1561 gtgatggtga acggcatgac catagacgag ttcaccggcc agggtatcac ggaaccagtg
     1621 gccgtgctgg cctagccaga tccataacgg cgcttattga ctgtgaggtt tgcagagtga
     1681 ctccgaatcc ggaagtgcga aaatatcaga tcgaccgttt gagaatcacc attttcccgg
     1741 agagaagtgc gatggggcag gcggccgccg ccggggtggc tgacaagatc cgcgcgctgg
     1801 ctgcccggca ggatgccatc cggatggtct ttgcggcagc gccatcccag aatgagttcc
     1861 tggatgccct gatcaagacc gagggcctcc cctgggcaaa aatcaccgcc tttcacatgg
     1921 atgaatatat cggactcgat cccgctgccc cgcagcgttt cggccagttc ctcagggcca
     1981 gactgttcga ccgcgtgcca ttcggccgta ttcactacct gaacccatcc acggaaaggg
     2041 tcgcgcaaga gtgcgagcgg tatactgcac tttttaccgc tgcacccatt gacatcatct
     2101 gcatgggcat cggcgaaaat ggccacattg cctttaacga ccccccggtg gccgatttca
     2161 atgatccccg gatagtcaag gtggtcgatc tggacccggt ctgccgccag cagcaggtca
     2221 atgatggctg ctttgcttcc ttgaatgagg tgcccaaaca agccataacc ctcacagtcc
     2281 cggcgctggc cgcgggcaaa agcctgttca tcatcgtacc gggaaaaacc aagagcaagg
     2341 ccgtgcatga caccctgctc ggcgaaatcg gcacccattg tccggcctcg atcctgcgaa
     2401 atcatcccga cgcggaactc tacctggatg cagactccgc tgccgcactg gcctgatcga
     2461 gttgttttac tctatgaaac gacgaggaac aaccatgcga aacaatactg taaagctttt
     2521 atccactctc ctgaccctta tgttcgtcca tctggcggcc ggtcagcctc cagccagtca
     2581 actcaatctc atgccggtcc cggaaaaggt cgagctcctt gcgggcaagt accggctgga
     2641 tgccggtttc acggtccgga ttgccggtgc acagagcggg cggctggtaa aaggggcatc
     2701 ccggatcctc gaccggctga ccggacggac cgggttgttc ctcgcgaacc ccacttcctt
     2761 gcttgatgcg agtgcaccgg ctgccggcat gctccttacg gccgggcggc aaggtgaact
     2821 tgccccgctg caagatgaat cgtatgatct ggtcatcacc ccggacggca tcaagatgac
     2881 cgcggagacg gatattggca tcatccgggg gctggaaacc gttctgcagc tgcttgacgc
     2941 tgatgaaaac ggctatttct ttccggccgt gcgggtccac gacaaaccca ggtttccctg
     3001 gcgcggcctg ctcatcgatg ccggccgtca tttcattccg gcggacatca tcagacgcaa
     3061 tctggacgcc atggcggcgg tcaagatgaa tgtgctgcac tggcatctta cggaggatca
     3121 gggattccgg atcgaaagca aggcctttcc aaagctgcac cagctcggat ctgacggtct
     3181 ttattacacc caggcggaga tcgccggtat cgtcgcctat gccggtgacc ggggcattcg
     3241 ggtaatgccc gaattcgacc tccccggcca tgccaccagc tggtttgtcg gctatccgga
     3301 actggcgagc gctccgggcc cctatacc
//
LOCUS       DAOPWU010000364         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_29303_length_3325_cov_5.256269, whole genome shotgun sequence.
ACCESSION   DAOPWU010000364 DAOPWU010000000
VERSION     DAOPWU010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29303_length_3325_cov_5.256269"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2124)
                     /locus_tag="PKM23_13710"
     CDS             complement(<1..2124)
                     /locus_tag="PKM23_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92574.1"
                     /translation="MKFLTFCTTMLTAALAAAQGPATAWYQAVTVPPSYAYHLSGRSD
                     RVHGYVVKVDDPAGLRTPGLILLASGIDAGEGTVEVNGKVYPLPALLGAANRQSDKSE
                     KNAEGKEGWYSFSSGDDIIGKVVIPLEASLLKAGLNDVTFFKAEGRDGFEVIDARIEA
                     VDAVAPQVVGQTYHILARGKPSSVGDFDYVYRYNGEKKRLEREIPEWRRRGKVIFYRA
                     GIDWDHLDRMFEMFREARVNDVAAHIPADSTTAEYQRVKAFIDRCHAGGIRVTAFNSL
                     GSISYRDLLMNPGLEKWISRDEYGTLRWRGEKGGNYAADLQNADYRKNALLKHAAVQM
                     DAGADELYYDWAIGGTGDVLEFFAEVRQLAKEKGINISIFGNCKGNILADEVCDETKS
                     EGTTEAGVWDGKWVHNVAQARFYYASGFGVKPYESKYEGADPGVPNPGAYDVREGMKM
                     GWRKPIAEASAFQSHFAIAEAGDKMLDGWVHKNNALAMEVWGQISRYMSFLADHQELY
                     TTVSCVSKLGIVAPPHIPSFEVSLTRASLYNALVEMNIMYDVLLLHRLTPAMLAPYKA
                     IIIPNITWMEESQLDAIRTYKQGGGKVYTIGCTPEVRGAADRYSHPEIFGRLGEENAR
                     TELARELQQLEGDPLVTLTDAPYVAANLVRKNGTNRFILHLVNYDKPLRNVRVRLDLT
                     GFSEKIDRKKIHCLSPDLEASIPVQA"
     gene            complement(2139..>3325)
                     /locus_tag="PKM23_13715"
     CDS             complement(2139..>3325)
                     /locus_tag="PKM23_13715"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HNY92575.1"
                     /translation="EYLDSAPRKNEEGVKVTNSPALKFLAAASHGKPVIIYATEITPP
                     IFPNPTDQALSAMAQINIAEAVANHCIFREKRLTPEGATRTYAFLAENEPVLLTCQLR
                     SNIAILASLNQYLADELSFAFISSRVLADRGIAHDLIVEKDLLDGTLQRYDLVVVPYI
                     PLLSLEKQQALVQYARSGGRVLVLGESGRKDQYGLEQTQNHFLKALRQKSWPTQYREK
                     KVGRGVLAFMPLPIPAGRYLVPAKAKSEFTTFGPSMADLFADIPEAYTRGRFNPQLLP
                     LLTSAVERIETLLKHRMSRLTTPAPFVEITTMLPETQGHMLLHLVNYNVTIDGLITPA
                     ENVGVQLLLPPGKQAVRVSCSGTLAERQPLAFKTDPKNPRLITFTVPRVEVYGMAVVE
                     LN"
BASE COUNT          601 a          966 c         1079 g          679 t
ORIGIN      
        1 cgcctgaacc gggatggacg cctccagatc cggagaaagg cagtggatct ttttccggtc
       61 gatcttttcc gagaaaccgg tcagatcgag cctgacgcgc acattccgca gtggtttgtc
      121 atagttgacg agatggagga tgaagcggtt ggtgccgttc ttgcgcacga gattggccgc
      181 gacatacggc gcatcggtca gagtgacaag gggatcgcct tcaagctgct gcagctctct
      241 ggccaattcc gtacgggcat tttcctcgcc aagccggcca aagatctcgg gatgggaata
      301 gcggtccgcc gctccgcgca cctcgggggt gcagccgatg gtatagacct ttccgcctcc
      361 ctgcttgtag gtccggatcg catccagctg gctctcctcc atccaggtga tattgggaat
      421 gatgatcgcc ttgtaggggg ccagcatggc gggggtgagg cggtgcagca gcagaacgtc
      481 gtacataatg ttcatctcga ccagggcgtt gtagaggctc gcgcgggtga gagagacctc
      541 gaagctggga atgtgcggcg gagcaacgat accgagtttg ctgacgcaag aaaccgtggt
      601 gtagagttcc tgatgatcgg ccagaaagga catgtaacgg ctgatctggc cccagacctc
      661 catggcgagg gcgttattct tgtgcaccca gccgtcgagc atcttgtcgc cggcctcggc
      721 aatggcgaaa tgggattgaa aggcggaggc ctcggcgatg ggcttgcgcc agcccatttt
      781 catgccctcg cgcacatcgt acgccccggg gttgggcacc ccgggatcag cgccttcata
      841 tttgctttcg tagggtttga ccccaaaccc cgaggcataa taaaagcgcg cctgggcgac
      901 attgtgaacc cacttgccat cccagacccc cgcttcggta gtgccttcgc tcttggtctc
      961 gtcgcagacc tcgtcggcga ggatgttgcc cttgcagttg ccgaagatgg agatattgat
     1021 gcccttttcc ttcgccagct ggcgcacctc ggcgaaaaat tcgagcacat ccccggtgcc
     1081 gccgatggcc cagtcatagt agagttcatc ggccccggca tccatctgca cggcagcgtg
     1141 cttgagcaga gcatttttgc gataatccgc attctgcagg tcagcggcgt agtttccccc
     1201 cttctcgccg cgccagcgca gggtcccgta ctcatcgcgg ctgatccatt tctccagccc
     1261 gggattcatg aggaggtcgc ggtagctgat gctgccgagc gagttaaagg ccgtcacccg
     1321 gatgccgccg gcgtggcagc ggtcgatgaa ggccttgaca cgctgatatt ccgcggtagt
     1381 ggagtcggcc gggatatggg ccgcaacatc attgacccgc gcttcgcgga acatctcgaa
     1441 catgcggtcg aggtggtccc agtcgatccc ggcgcggtag aaaataacct tgccgcgccg
     1501 ccgccactcc gggatctccc gctcgagccg cttcttctcg ccattgtagc ggtagacata
     1561 atcgaaatcc ccgaccgaag agggcttgcc gcgcgccaga atatgatagg tctgcccgac
     1621 gacttgcgga gcgaccgcat cgaccgcctc gatgcgagcg tcgatgacct caaagccatc
     1681 ccggccctct gccttgaaaa aggtgacgtc gttgagcccc gccttaagca gtgatgcttc
     1741 gagggggatc accaccttgc cgatgatatc gtcgccgctg ctgaaggagt accacccctc
     1801 cttgccctcg gcgttcttct cgctcttgtc cgattgccgg ttggctgcgc cgagcagggc
     1861 gggaagggga tagaccttgc cgttgacctc gacggtgcct tcgccggcat cgatgccgct
     1921 ggcgagcaga atcagaccgg gggtgcgcag tccggccgga tcatcgacct tgaccacata
     1981 gccgtggacg cggtcgctgc gcccggagag atgataggca taggagggcg gcaccgtgac
     2041 cgcctggtac caggcggttg ccgggccctg ggccgccgcc agtgcggccg tcagcatcgt
     2101 tgtacaaaaa gtcagaaatt tcatgcactc ctccgggttc agttcagttc caccaccgcc
     2161 ataccataga cctcaaccct gggtacggtg aaggtgatca gtcgcggatt tttcgggtcg
     2221 gtcttgaagg cgagcggctg gcgttccgcc agggttcccg aacagctcac ccgtacggcc
     2281 tgcttgcccg gcggcagcag cagctgcacc ccgacattct cggcgggggt aatgagtccg
     2341 tcgatggtca cgttatagtt gaccaggtgg agcagcatgt gtccttgcgt ctccggcagc
     2401 atggtggtga tctcgacgaa gggggccggc gtggtcagcc ggctcatgcg gtgcttgagc
     2461 agggtttcga tccgctccac agcggaggtc aggagcggca gcagctgcgg attgaaacgg
     2521 ccgcgggtgt aggcctcggg gatatcggcg aagagatcgg ccatggaggg gccgaaggtg
     2581 gtgaactcgg acttggcctt ggccggcacc aggtagcggc ctgccggaat gggcaagggc
     2641 ataaaggcga gcacgcccct gcctaccttc ttctcgcggt actgcgttgg ccagcttttt
     2701 tgcctgagtg ctttcagaaa atggttttgt gtttgctcca gtccatactg atccttgcgg
     2761 ccgctttcgc ccagcaccag cacgcggccg ccgctccggg catactgcac cagggcctgc
     2821 tgcttttcaa ggctgagcag ggggatatag ggaaccacga ccagatcgta gcgctgcagt
     2881 gtgccatcga ggaggtcctt ttcgacgatg aggtcatggg cgatgccgcg atccgccagg
     2941 acccgggagg agataaaggc gaaagagagc tcgtcggcga ggtactggtt gagcgaggcg
     3001 aggatggcga tattgctgcg cagctggcag gtgagcagca cgggctcatt ctctgccagg
     3061 aaggcatagg tgcgggtggc cccttcaggg gtgagccgtt tctcgcgaaa gatgcagtgg
     3121 ttggcgaccg cttcggcgat gttgatctgg gccatggcgc tcagggcctg gtctgtggga
     3181 ttgggaaaga tcggcggcgt gatctcggtc gcatagatga tcaccggctt gccatgcgag
     3241 gcggcggcga ggaatttgag cgccgggctg ttggtgacct tgacgccttc ctcgttttta
     3301 cgcggggcgc tgtcgagata ctcct
//
LOCUS       DAOPWU010000365         3317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_29409_length_3317_cov_4.123850, whole genome shotgun sequence.
ACCESSION   DAOPWU010000365 DAOPWU010000000
VERSION     DAOPWU010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29409_length_3317_cov_4.123850"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PKM23_13720"
     CDS             <1..785
                     /locus_tag="PKM23_13720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY92576.1"
                     /translation="SGRFWHPLAGALVQLARFGRFVSLSLYWLVVAPFRGHRIRWGSA
                     FVQMMRMGVGSLPVVGTISFFTGLILAMQSAQQLEPFGAVIYVADLVGVAITRELGPL
                     LTAILVAGRCGSAVTAEIGSMRVSEELDALSTMALNPFAFLVVPRTLAMMIMLPCLTV
                     MSDFLGILGGAILAVTTLKIPFIAYYNETVTALVMADFLTGLFKSLVFGFIIVMIGAY
                     QGFNVEGGAEGVGKSTTASVVSSIFLIILADLIFTALFYSTF"
     gene            complement(782..>3317)
                     /locus_tag="PKM23_13725"
     CDS             complement(782..>3317)
                     /locus_tag="PKM23_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92577.1"
                     /translation="LLDACGDNVWWHIKRNYHWPQEWSTITIKRRHIDFAWGPAADRN
                     LRRMDKLEFMISSSSGGRGTVWIDDLSFQALPPEQPPTPAEITGAARRRDLGFFESPD
                     SFFNALAREEPAGLYPRYCRGEQSFWTVVGVNNARQEALINSDGMIEVDKGGFSIEPF
                     LAVEDSLYSWHQARSAASLAGGCLPLPRVDWEGPPLRLTVEAFADGPERDDPAAAGRG
                     CLYLTYTLTNPASRPSDAQLYLALRPFQVNPPWQFLNWPGGTAPIRKIEFRGDRAVVN
                     DEKIIRFSTPPEAFGAAAFAEGDISSYIGKGLLPEGQTVTDDRTLASGAARYSWRLQP
                     GASRQVSLIIPFGAHDPGAAAADIPRLRKDVEAFWRGKISTVSIHLPASAQEVMETLQ
                     ANLAWILINRDSAGIQPGSRSYERSWIRDGALTSAALLRFNIRREVRDFLDWYSRHLY
                     PSGKVPCVVDRRGADPVPENDSNGEYLFAMRQYFLFSADTAFIRARYPAIRAAAAWLD
                     SLTARRMTSRYLPVGEDSSDAFYGLVPESISHEGYSAKPMHSYWDNFFTLRGYNDAVE
                     LARLLGQTADEKWLRRSRDRFRENLLASLERAIRYKKIDYLPGCVELGDFDPTSTAIA
                     LYPGNLADLLPQPQLNNTFDRYYDFFTRRRDGLIHWRDYTPYEVRTIGAFVRLGQPER
                     AHALLDFFMQDRRPPGWRHWAEVVWPDPKTPRFIGDMPHTWVGSDFINSVRTMFLYEE
                     EHRDALVIGAGLRREWISEGEGVRVEGLPSYYGPVSYHYIGKGNGCRIEISGGLRLPP
                     GGIEVVHHQAGRNLKVTVNGRSWREFDASAVRLRSLPAVIEVSTGD"
BASE COUNT          517 a         1006 c         1170 g          624 t
ORIGIN      
        1 tctccggccg gttctggcat cccctcgccg gcgccctcgt gcagctggcg cgcttcggcc
       61 gcttcgtctc gctttccctc tactggctcg tggtcgcgcc ctttcgcggc caccgcatcc
      121 gctggggctc cgctttcgtc caaatgatgc ggatgggggt cggctccctt cccgtcgtcg
      181 gcaccatctc tttcttcacc ggcctcattc tggccatgca gtcggcccag cagctcgagc
      241 ccttcggggc ggtcatctat gtggccgacc tggtcggggt ggcgatcacg cgcgaactgg
      301 gcccgcttct caccgctatt ctcgtcgccg ggcgctgcgg ttcggctgtc accgccgaga
      361 tcggcagcat gagggtctcc gaggaactcg acgcgctttc gacgatggcc ctcaatccgt
      421 tcgcctttct ggtggtgccc cgcaccctgg ccatgatgat catgctcccc tgcctgaccg
      481 tgatgagcga ttttctcggc atcctcgggg gggcgattct ggccgtgacc accctcaaga
      541 tccccttcat cgcctactac aacgagaccg taaccgccct ggtgatggcc gatttcctca
      601 ccggactctt caagagcctg gtcttcggct tcatcatcgt catgatcggc gcctaccagg
      661 gtttcaatgt cgaaggcggg gccgaggggg tcggcaagtc gaccacagcc tcggtcgtga
      721 gctcgatttt tttgatcatc ctcgccgacc tcatcttcac cgctcttttt tattcgacct
      781 tttaatcccc cgtgctcacc tcgatcacag ccggcaagct gcgcagccgg acagcgctgg
      841 cgtcgaattc cctccagctg cggccgttga ccgtcacctt tagattccgg cccgcctgat
      901 ggtggaccac ctcgatcccc ccaggcggga gccgcagccc gcccgatatc tcgatgcggc
      961 agccattccc cttcccgatg taatggtagc tgaccgggcc atagtagctc ggcagcccct
     1021 ccacgcgcac gccctctcct tctgaaatcc attcgcggcg caggcccgcc ccgatcacca
     1081 gggcatcgcg atgctcctct tcgtagagga acatcgtccg caccgaattg atgaaatcgg
     1141 agccgaccca ggtgtggggc atgtcgccga tgaagcgggg ggttttgggg tcgggccaga
     1201 ccacctccgc ccagtggcgc cagccgggcg ggcggcggtc ctgcatgaag aaatcgagca
     1261 gggcgtgggc gcgttcgggc tggccgagac ggacgaaggc tccgatggtg cgcacctcat
     1321 agggggtgta gtcgcgccag tggatgagtc cgtcgcggcg ccgggtgaaa aagtcataat
     1381 agcggtcgaa ggtattgttg agctgcggct gcggcaggag atcggcgaga ttgccggggt
     1441 aaagggcgat ggcggtcgag gtggggtcaa agtcaccgag ttcgacgcag ccgggcaggt
     1501 agtcgatctt tttgtagcgt atggcgcgct cgagggaagc gagcagattt tctcgaaagc
     1561 ggtcccgcga gcggcgcagc cacttctcgt ccgcggtttg gccgaggagg cgggcgagtt
     1621 cgacggcgtc gttgtaaccg cgcagggtga aaaagttgtc ccagtaggag tgcatgggct
     1681 tggcggagta gccctcatgc gagatcgact cgggcacgag gccgtaaaag gcgtcactcg
     1741 agtcctctcc gaccggcagg tagcggctgg tcatgcgccg ggcggtgagg gagtcgagcc
     1801 aggccgcggc ggcgcggatg gcgggatagc gagcgcggat aaaggcagta tcggcgctga
     1861 agagaaaata ctggcgcatg gcgaagaggt attcgccgtt gctgtcattt tcgggcacgg
     1921 ggtcggcccc gcggcggtcg accacgcagg gcaccttgcc gctggggtag aggtggcgcg
     1981 aataccagtc gaggaaatcc ctgacctcgc ggcggatgtt gaagcggagc agggcggcgg
     2041 aggtgagggc gccgtcgcgg atccaggagc gttcatagga gcgcgaaccg ggctggatgc
     2101 cggcgctgtc ccggttgatg aggatccagg cgagattggc ctggagggtc tccattacct
     2161 cctgcgcgga ggccggcagg tggatggaga cggtgctgat tttaccgcgc cagaaagcct
     2221 ccacatcttt gcgcaggcgg gggatgtcgg cagccgccgc gccggggtcg tgggcgccga
     2281 aggggatgat caggctgacc tgccggcttg cgcccggctg caggcgccag gagtagcgcg
     2341 ctgccccgga ggccagggtg cggtcgtcgg tgactgtctg gccctcgggc agcaagcctt
     2401 tgcctatata gctgctgata tccccttcgg cgaaggcggc cgctccaaag gcctccgggg
     2461 gcgtgctgaa gcggatgatt ttttcgtcgt tgaccacggc gcggtcgccg cggaattcga
     2521 tttttcttat cggggcagtg cctcccggcc agttgaggaa ctgccagggg ggattgacct
     2581 ggaaggggcg cagggcgagg tagagttgtg catcgctggg ccgtgacgcg gggttggtga
     2641 gggtgtaggt cagatagagg cagccgcggc cggcggcggc ggggtcgtcc cgttccgggc
     2701 cgtcggcgaa ggcctcgacg gtgaggcgca gcgggggccc ctcccagtcg acgcggggga
     2761 ggggcaggca gccgccggcc aggctggcgg cgctgcgggc ctggtgccag ctgtagaggc
     2821 tgtcctccac ggccagaaac ggttcgatcg aaaagccccc cttgtcgacc tcgatcatac
     2881 cgtcgctgtt gatgagggcc tcctggcggg cgttgttgac cccgacgacg gtccagaagg
     2941 actgctcccc ccggcagtag cggggataga gcccggccgg ctcttcgcgg gccagggcgt
     3001 taaaaaagga gtccgggctc tcgaaaaagc caagatcgcg gcgccgggcg gcgccggtga
     3061 tctcggcggg ggtcggtggc tgctccgggg gcagggcctg gaaggagagg tcgtcgatcc
     3121 agacggttcc gcggccgccg gaggaggagg agatcatgaa ttcgagtttg tccatcctgc
     3181 gcaggttgcg gtcggctgcg ggtccccagg cgaagtcgat gtggcgccgt ttgatcgtga
     3241 tcgtgctcca ctcctgcggc cagtgatagt tgcgtttgat gtgccaccag acgttgtcac
     3301 cacaggcgtc gaggagc
//
LOCUS       DAOPWU010000366         3306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_29534_length_3306_cov_4.063365, whole genome shotgun sequence.
ACCESSION   DAOPWU010000366 DAOPWU010000000
VERSION     DAOPWU010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29534_length_3306_cov_4.063365"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKM23_13730"
     CDS             <1..365
                     /locus_tag="PKM23_13730"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNY92578.1"
                     /translation="AEIGRLAPRQLDMVYECTGDAEALRQGIRLLKPGGRLVIVGIPE
                     ENEIVLPIHELRRREIDILNIRRQAGATQKAVDLLESGALRMADMATHHFPLVEAAAA
                     FKLTAARADGVIKAMITL"
     gene            441..1775
                     /locus_tag="PKM23_13735"
     CDS             441..1775
                     /locus_tag="PKM23_13735"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HNY92579.1"
                     /translation="MKPLPRLLLSALFLAGAFSPALPAATEGTPHDYTVVKNLEWVKV
                     LNKSLTMDIYTPSTGRSSYPVLVIYHGGGWLINTNAIMDSMAVYMVRHAETVVCNVNF
                     RLLGDLGNTVTMNQIIEDALGAIAWIKEHISAYQGDATRLIVTGDSSGGHMAAMVLLA
                     SDHLESDGFKGTTLGFCPTWLPPSKTAEELAASHALDVQGAILSYGAFDIYAACRSGL
                     ETSSNIFWALAGKTARRIFGVSYSILANPEMYKAVSPAYIIPKASARPLPPQLCLVGS
                     KDTTTPPATVKAYVDSTMKAGQPIEYWEYAGKPHAFLDATPNSWLGTNFTGDAPAALD
                     KMIAWMSVNRLTAVGSKMQRPESDFRLDQNYPNPFNASTSISFYLDEADQIRLTVHDL
                     QGRRICTLADGFQSAGPKLFRFDAAGLAGGIYFCRMESGRGRSRTMRMLLLK"
     gene            complement(1886..2917)
                     /locus_tag="PKM23_13740"
     CDS             complement(1886..2917)
                     /locus_tag="PKM23_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92580.1"
                     /translation="MKYVNRILLFLSLFVLYIIAKEFLQIYTMLHAVHPALAIGFSLL
                     ALAAFIYFVGIPLFRIISMPVSYGPVYDPGQEERLIAKRIKRFKKNKYLIRSGFDVAS
                     LQNDRASYDRVIAEMDKAAEQQRRKDIPQLFYATAIVQNGFLDALLILSAAINHIKEL
                     FILYNGRVSNRDLIVILKKIYFAMAIGGSEAAEYATEEIFSRFATDGMKSIPFVSKVS
                     SSLVDGYINAVMLTRISYITENYCRKTVIASDKELLPSSEFIFDSARTITEDIRSKIY
                     EAFKKRTKGRIAKALEPVTGFFQKTFDFVTPQEIDPENHYGLEMPGGGTEPPVSYGFW
                     KLFESLRKS"
     gene            complement(3033..>3306)
                     /locus_tag="PKM23_13745"
     CDS             complement(3033..>3306)
                     /locus_tag="PKM23_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNY92581.1"
                     /translation="APRTNPYLGTQESYGGGKPFSEETARLIDAEVQKIISDCHEEAK
                     RLLREHRAELDALAAALVARETLDEQEILAATGLPPAPPLETGKLP"
BASE COUNT          668 a          967 c         1003 g          668 t
ORIGIN      
        1 aggcggagat cggccggctt gcgcccaggc agctcgatat ggtctacgag tgcacagggg
       61 atgctgaggc gctccgccag ggcatccgtc tgctgaaacc gggaggccgc ctggtcatcg
      121 tcggcattcc ggaggagaac gagatcgtcc tccccatcca tgagctgcgg cgccgggaga
      181 tcgacatcct caacatccgc cgacaggccg gcgccacaca aaaggcggtc gatctgctcg
      241 aaagcggagc gctcaggatg gccgatatgg ccacccacca ctttccactc gtggaggccg
      301 cggcggcctt caagctcacc gccgcccgtg ccgatggcgt catcaaggcg atgatcacgt
      361 tgtagatcag gacagagctt ctgcaaagcc tcactccacc ttccaaaggc cggttcacct
      421 aacgattcca ggagatcccg atgaaacctc tccctcgttt gctcctttcc gcgttgtttc
      481 tggcgggcgc cttctcccct gccctgccgg ctgcaaccga aggcacgcca cacgattata
      541 ccgtcgtcaa gaacttggaa tgggtcaagg tgctcaataa aagcctgacc atggacattt
      601 acacgccatc caccggcagg tcctcctacc ccgtgctggt catctaccac ggcggcggct
      661 ggctgatcaa taccaacgcc atcatggaca gcatggcggt ctatatggtg cgccacgcag
      721 aaacagtggt ctgcaacgtc aattttcgac tgctcggcga tctcggcaac actgtcacca
      781 tgaatcagat catcgaggac gcactggggg ccatcgcctg gatcaaggag cacatcagcg
      841 cctatcaggg ggatgcgacc aggctcattg tcaccgggga cagctcgggc gggcatatgg
      901 cggccatggt cctgctcgcc agcgaccacc tcgaatcgga tggctttaag gggaccacgc
      961 tgggattttg tccaacctgg ctgccgccca gcaaaaccgc cgaggaactg gccgcgagcc
     1021 atgctctgga tgtccagggt gctatcctca gttatggcgc ctttgacatt tacgccgcct
     1081 gcaggagcgg tttggaaaca tccagcaaca tcttctgggc gctggccgga aaaacagcgc
     1141 ggcggatctt cggcgtctcc tacagcattc tggcaaaccc cgaaatgtac aaggcggtct
     1201 cgcccgctta tatcatcccc aaggccagtg cacgtccgct gccgccgcag ctatgtctcg
     1261 tcggctccaa agacacgacc acgccgccgg ccacggtcaa ggcctatgtc gattcgacga
     1321 tgaaggccgg ccagccgatc gaatactggg aatacgccgg caagccgcac gcctttctcg
     1381 atgccacgcc caactcctgg ctgggcacaa atttcaccgg ggacgccccg gctgccctgg
     1441 acaagatgat cgcctggatg tccgtcaatc gacttactgc agtcggaagc aaaatgcaaa
     1501 ggcctgaatc ggactttcgg ctcgaccaga actatcccaa ccccttcaac gcctcaacca
     1561 gcatctcttt ttaccttgat gaagcggacc agatcaggct gacggttcat gaccttcaag
     1621 gccggaggat ttgcaccctg gccgacggat tccagtccgc tggccccaag ctcttccgtt
     1681 ttgacgccgc agggctggcc ggcggaatct atttctgccg catggagagc ggccgcggca
     1741 ggagtcggac gatgcgcatg ctgctgctca agtgagtaca gcaccgcctc gcctggaaac
     1801 cgctcactga cggttatact ggtgtcatct ttatgccggt catagggcaa aggcgggaat
     1861 caggagcgca atggtggtat cctgctcagc tcttcctcag gctctcgaac agcttccaga
     1921 agccataact gaccggcggc tccgtgccgc ctccgggcat ctcgaggcca tagtggtttt
     1981 cgggatcgat ctcctggggg gtgacgaaat cgaaggtttt ctgaaaaaag cccgtaaccg
     2041 gctccaaagc cttggcgatg cgccccttgg tgcgcttttt aaaggcttcg tagattttgg
     2101 agcggatgtc ctcggtgatg gtgcgcgccg aatcgaagat gaattccgag ctcggcagca
     2161 gttccttgtc ggaggcgatc acggttttgc ggcagtaatt ctcggtgatg taggagatgc
     2221 gggtgagcat gacggcgttg atatagccgt cgaccagcga ggaggagacc ttgctgacga
     2281 aggggatcga tttcatgccg tcagtggcga agcgggaaaa gatctcctcg gtggcgtatt
     2341 cggccgcctc cgagccgccg atggccatgg caaagtagat ctttttgagg atgacaatga
     2401 gatcgcggtt ggagacccgg ccgttgtaga ggatgaagag ttccttgatg tggttgatgg
     2461 cggccgagag gataagcagg gcgtcgagaa agccgttctg gacgatggcc gtggcgtaaa
     2521 agagctgcgg gatatccttg cgccgctgct gttcagccgc cttgtccatc tccgcgatga
     2581 cccggtcata gctggcgcga tcgttctgca gggaggccac atcgaatcca ctgcggatga
     2641 gatacttgtt ctttttaaaa cgcttgattc gtttggcgat cagccgctcc tcctggccgg
     2701 gatcgtaaac cggtccatag ctgacgggca tgctgatgat cctgaagagg gggatgccga
     2761 cgaaatagat aaaagccgca agtgccagca gtgaaaatcc gatcgccagg gcgggatgaa
     2821 ccgcatgcag catggtatag atctgcagaa attccttggc aatgatataa aggacgaaaa
     2881 gggagaggaa aagaaggatc cggtttacat atttcatgtt cagctcccgg gattcagcag
     2941 cggcggggag acgcctgcgc ggcggccgct gctgcagatc ctgacttatg cctggccgta
     3001 ccaggcgttg atgtgttgat ctccggccgg tttcagggca acttgccggt ttcgaggggc
     3061 ggcgccggcg gcagcccggt ggcggcgagg atctcctgct cgtcgagggt ctccctggcg
     3121 accagggcgg cggcgagggc gtcgagttct gcgcggtgct ccctgagcag ccttttggcc
     3181 tcttcatggc agtcgctgat gatcttttgc acctcggcgt cgatcaggcg cgcggtctct
     3241 tcgctgaagg gcttgccgcc gccatagctc tcctgggtgc cgagataggg attggtgcgc
     3301 ggagcg
//
LOCUS       DAOPWU010000367         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_29738_length_3288_cov_4.312713, whole genome shotgun sequence.
ACCESSION   DAOPWU010000367 DAOPWU010000000
VERSION     DAOPWU010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29738_length_3288_cov_4.312713"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1813
                     /locus_tag="PKM23_13750"
     CDS             <1..1813
                     /locus_tag="PKM23_13750"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HNY92582.1"
                     /translation="MKLPSIRQMAEELKRIIIRFPLVMASAAAGTIAALVLIEQQGPT
                     KPTIWFPILLTSLVGFFLLLGLAMLAEKRRWPPALRIASQGGGVLLLLLYGTSLPLLP
                     EGEPAYHIVRFLLLMAAVVIFALTIPFAGTKEENGFWHYGRILVVRFLTAAFYAAVLQ
                     ISLSLALLALNQLFDITIPGERYGELWALLVGLFGTSFFLAGMPGELNALEGRNDYPK
                     GLRIFAQNILSAVVLVYLVILYAYLAKIILAWSWPRGWVSGLIIGFSATGLAALLLLW
                     PIRSQEENRWGHLVWRWFFIVLIPLVVMLFLAVWRRISEYGLTVSRCLVVAVALFLAL
                     LILYFLISKAKSIRVIPGLFGIIALLLSFGPWGVFSLSEQSQKGRLETLLSRNGLLEG
                     GTIRQAAGAVPDQDIVQISSILSYLNEMHGYGAIQSWFREPLKEEKEGRPVRLRPEEV
                     TKKLGVEYMRGARSGFGSERTLRARKQSYFDIAGYDRMVTGLFTGEAGELYGLEDAGV
                     TCTVSKGLKQLTLVVKEGEGAGESLTYELLPRVESLYAEWGLTNPEAIPLGELAIDAE
                     GEHLKARLYLRHIQLEQKEEGLEIRNLSMDLLYALKP"
     gene            complement(1845..3191)
                     /locus_tag="PKM23_13755"
     CDS             complement(1845..3191)
                     /locus_tag="PKM23_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNY92583.1"
                     /translation="MFMGDDYIDRIPEDLDGDAAALVERFINQWSRGTTFRSLDGRDR
                     SELLTRMVQIVHAMANPEDYRAIAGRILEACLETVPADSAVLLVHNAQGKLAVTMTRG
                     GEASDALLKEHGGIIQKVLRSRERFILEQEGKLATGPGARQRCFFPVVSSRNLGGILF
                     LDGVLLTEEREVPAAAQIWSGMLGLLLSHSQLHHSKELLNGYLRNMQEKVIWFDKVAG
                     NGRIAASAGHELNNLLSVLAGNLELAKSWLQAGEEPGRILERLGLLQDVVASAAQISQ
                     GLINPEPQEKQMQRCSMNNLVCETVELLKPLVTRRGARFDLQPGAELPDVLVDATQIR
                     QVVRNLLLNAVEARSDVQIWIRLSHDPEAQRIKLAIRDNGPSIPAARIPALFSPLQAG
                     QEKGLSLLICKEIIERHGGSLRFESEEGAGSEYTISLPQYGAETQLCWRKKINRSE"
BASE COUNT          568 a         1023 c         1012 g          685 t
ORIGIN      
        1 catgaaactc ccctcaatcc ggcagatggc tgaagaactc aagcgcatca ttatccgttt
       61 tcccctggtc atggcctccg cagcagccgg caccatcgcc gccctggttt tgatcgaaca
      121 gcaggggcca acaaagccga cgatctggtt cccgatcctg ctcaccagcc tggtcggctt
      181 tttcctcctg ctggggctgg ccatgctcgc cgaaaagcgc cgctggccgc ccgccctgcg
      241 catcgcctcc cagggcggcg gcgtcctgct gctcctgctc tatggcacct cactgccgct
      301 gctccctgaa ggcgaacccg cctatcacat cgtgcgcttt cttttactca tggctgcggt
      361 ggtgattttc gccttgacga tcccctttgc gggtaccaaa gaggaaaacg gcttttggca
      421 ctatggccgg atcctcgtgg tccgtttcct tactgccgct ttttatgccg cggtgctgca
      481 aatcagcctc tccctggccc tcctcgccct caaccagctc ttcgacatca ccatccccgg
      541 agagcgctat ggtgaactct gggccctcct cgtcggactt ttcggcacct ctttcttcct
      601 agccggcatg cccggggagc tcaacgcact tgaagggaga aatgactatc ccaagggact
      661 ccggatcttt gcgcagaaca tcctctcagc ggtggtgctg gtctacctgg tgattcttta
      721 cgcctacctg gccaagatca ttttagcctg gtcctggccg cggggctggg tgagcggact
      781 gatcatcggc ttttccgcga ccggtcttgc cgccctgctc ctgctctggc cgatccgcag
      841 ccaggaggag aaccgctggg gccacctggt ctggcgctgg ttcttcatcg ttctcattcc
      901 cctggtggtc atgctcttcc ttgccgtctg gcgcaggatc tccgaatatg gcctgaccgt
      961 cagccgctgc ctggtggtgg ccgtcgccct cttcctggca ctgctcatcc tctatttcct
     1021 catcagcaag gccaaaagca tccgggtgat cccggggctg tttggcatca tcgccctgct
     1081 gctcagtttc ggcccctggg gggtcttctc cctctccgaa cagagccaga aggggcgtct
     1141 tgaaaccctg ctcagccgca acggcctgct cgagggcggg actatccgcc aggccgccgg
     1201 ggcggtgccg gaccaggaca ttgtgcagat cagctcgatc ctctcctatc tgaacgagat
     1261 gcacggctat ggcgccatcc aatcctggtt cagggagccg ctgaaggaag aaaaagaggg
     1321 caggccggtc cgcctcagac cggaggaggt gaccaaaaag ctgggtgtgg aatatatgcg
     1381 aggcgcgcgc agcggttttg gctccgagcg caccctgcgc gccaggaagc agagctattt
     1441 tgatatcgcc ggctatgacc gcatggtgac cgggcttttt accggcgagg ctggcgagct
     1501 gtatggactg gaggatgcgg gggtgacctg tacggtctcc aaggggctga agcagttgac
     1561 cctggtggtt aaggagggtg aaggcgccgg ggaaagcctc acctacgagc tgctgcccag
     1621 ggtggagagt ctttatgcgg aatggggcct taccaatccg gaggcgatcc ccctgggcga
     1681 gttggctatc gatgccgagg gagagcatct caaggccagg ctttacctgc gccacatcca
     1741 gctggagcaa aaggaggagg ggctggagat caggaatctg tcgatggacc tgctctacgc
     1801 cctcaagccc tgaagcttca gtgcagggtg cggcagaggt tgtttcactc gctccggttg
     1861 atctttttgc gccagcagag ctgggtttcc gccccgtact ggggcaggct gatggtgtac
     1921 tcgctgccgg cgccttcctc gctttcgaag cgcagggagc cgccgtggcg ttcgatgatc
     1981 tccttgcaga tgagcaggct cagtcccttc tcctggccgg cttgcaaggg cgaaaacagg
     2041 gccggaatcc gggcggccgg aatggagggc ccgttatcgc gtatggcgag cttgatgcgc
     2101 tgcgcctcag ggtcgtggct gaggcggatc cagatctgca cgtcgctgcg cgcctcgacg
     2161 gcgttgagca gcaggttgcg cacaacctgg cggatctggg tggcatcgac gagcacgtcg
     2221 ggcagttcag ccccaggctg gagatcgaaa cgggcgccgc gccgggtgac cagaggcttg
     2281 agcagctcga cagtttcgca gaccagattg ttcatggaac agcgctgcat ctgcttctcc
     2341 tggggttcag ggttgatgag tccctggctg atctgggcgg cgctggctac aacatcctgg
     2401 agcaggccaa ggcgttccag gatccgcccg ggctcctcac ctgcctgcag ccagctcttc
     2461 gccagttcca gattgcccgc cagcacgctg agcagattgt tcagctcatg accggctgag
     2521 gcggcgatcc ggccgttgcc ggccaccttg tcaaaccaga tcaccttttc ctgcatgttg
     2581 cgcagatagc cgttgagcag ttccttgctg tgatgcagct gggaatggct gagcagcagg
     2641 ccgagcattc cgctccagat ctgggctgcg gcgggcacct cccgctcttc ggtgagaagg
     2701 acgccgtcga ggaaaagaat gccgccgaga ttcctgctgc tcaccaccgg aaaaaagcag
     2761 cgctgccgcg ctccgggacc ggtagcaagc ttcccttcct gctccaaaat gaacctctcc
     2821 cggctgcgca ggaccttctg aatgatcccg ccgtgttcct tcagcagggc atcggaggct
     2881 tcgcccccgc gggtcatggt cacggcgagc ttgccctggg cgttgtgtac gagcaggacg
     2941 gcgctgtcgg cgggcacagt ctcgagacag gcctcgagaa tgcggccggc gatggcgcga
     3001 taatcctccg ggttggccat ggcatgaaca atctgcacca tccgcgtcag cagctcgctg
     3061 cgatcccgac catcgagaga gcggaaggta gtaccgcggc tccactggtt gatgaatcgc
     3121 tcgacgaggg ccgcggcgtc cccgtcgagg tcttccggaa tccggtcaat atagtcatct
     3181 cccatgaaca tggtggctcc tgtgcaaaca tgaaagggat gaggctgaga gcgggcagaa
     3241 ccggcctcgg ttctacccca tccatgccac aaatattgca tttgttgg
//
LOCUS       DAOPWU010000368         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_29763_length_3286_cov_5.670690, whole genome shotgun sequence.
ACCESSION   DAOPWU010000368 DAOPWU010000000
VERSION     DAOPWU010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29763_length_3286_cov_5.670690"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2639
                     /gene="sprA"
                     /locus_tag="PKM23_13760"
     CDS             <1..2639
                     /gene="sprA"
                     /locus_tag="PKM23_13760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNY92584.1"
                     /translation="VENLLPGDTGAIQKVFYEKQNYIHYNTLKMYTYGYDPSGTHITD
                     DSSSVEFFLQFGADKNNYYEVRQRVYDGWDKRNDIEIDLIELSAIKLIADYYDSSATR
                     KGYYLDIGGGKSYFIKGIPALTNVKMLVAGVRNLDPHDPFTGQVYMNELRLSNVKKDK
                     GMAYRARVEMNWAGLLNFNGELEKQDADFHNVATQYGEGNNSLSGSFSTSMALDKLLP
                     AKLGLSIPVSYNYGRSESTPKYKPGTDVEVTDALPDSILESIRTISQRQGYSVSLGMN
                     SRSKNFVVKNILNRLRASYSESRDRGSNSTMKSTSNRTQNGDLSWDVAFSPNNYIRPF
                     KWLGDARWLIKLADMKLFYTPQSFNSRISGTRTNSLSLTRTGVETDNGTFAVNSSIGG
                     SMKVFESLSLDLNRTYANDLYDIPPDSLRMMLDRGQLGYLTNMNQNFKIGYNPKIFNW
                     LTNNFTYSAGFRYGFNRQQKVQSRNAQLAKTLGVNGSLDLENLWASIYKPAGAVKGGK
                     RPTPAARRPAPGKEGTAGEEQKKEKEKEKEKGDGSGFSVMSLFSKFFGFFEPFSITYN
                     QRENLTTYGISRMPGMSFMLGLQDSLGVPIESAVGASGSSSSVNRNTSSTNENVHISS
                     GFSITRNIKVSLNYDNSASLNRSTTTTGQRSNSRLIYGEDIDMPFPQWTLRISGLEKL
                     PLINKYVQTMSLDHGYSGQFDQTYNMENGKEVITKEDRNSKFSPLAGMSLSFKNGMTM
                     SMRYTSGENNSLSSGYSVGGTRTTTNDLSVSAQYRKQSNFRIPIPVWPFKNMRLKNSV
                     NLQVTISSKSNVTRKSRAGGEYEITAETSQWLLKPDLQYSFSERVNGGMFLELGKTHN
                     KLIGDTSYYEFGLNANISIRGR"
     gene            2771..>3286
                     /locus_tag="PKM23_13765"
     CDS             2771..>3286
                     /locus_tag="PKM23_13765"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNY92585.1"
                     /translation="MKLSRLVIGVALISAGLALSCSAQNPPPFDGARAYTVLQKQCDF
                     GPRVPGSAGHRSCRDYLVTALRGYAAQVSTQEFIHTFGQPPQSARATNIIANFQPEKG
                     ERILLCAHWDSRPWADQDPDPANHSKPILGANDGASGVAVLLEITRLIRQKPPRCGVD
                     IVLFDAEDQGLE"
BASE COUNT          755 a         1058 c          891 g          582 t
ORIGIN      
        1 acgtcgagaa ccttctgccc ggcgacaccg gggcgattca aaaggtcttt tacgaaaagc
       61 agaattatat ccattacaat accctcaaga tgtacaccta tggctacgat ccctccggca
      121 cccatatcac cgacgactct tcctcggtcg agttctttct ccagttcggc gccgacaaga
      181 acaactatta cgaggtcagg cagcgggtgt atgacggctg ggacaagcgc aatgacatcg
      241 agatcgacct gatcgaacta tcggcgatca agctgattgc cgattactac gactcgtcgg
      301 caacccgcaa gggttattat ctcgatatcg gcgggggcaa gagctatttt atcaagggca
      361 tcccggcgct gaccaacgtc aagatgctcg tcgcgggtgt gcgcaacctc gatccccatg
      421 atcccttcac cggccaggtc tacatgaacg agctgcgcct ctccaacgtc aaaaaagaca
      481 agggcatggc ctaccgcgcc cgcgtcgaga tgaactgggc cggcctgctc aatttcaacg
      541 gcgaactcga gaaacaggac gctgattttc acaatgtggc cacccagtac ggcgagggca
      601 acaacagcct cagcggcagc ttcagcacca gcatggctct ggacaagctg ctgccggcca
      661 agctgggtct ctccattccg gtcagttaca attacggccg cagcgaatcg acgcccaagt
      721 acaagccggg caccgatgtt gaggtgaccg atgccctgcc cgattcgatc ctcgagtcca
      781 tcaggaccat cagtcagcgc cagggctaca gcgtcagcct cggcatgaat tcgcgctcca
      841 agaatttcgt cgtcaagaat atcctcaacc gcctccgtgc cagctacagc gaatcgcgcg
      901 accgcggcag caactcgacc atgaagtcga cttccaatcg cacccagaac ggcgatctga
      961 gctgggatgt tgccttcagc ccgaataact atatccgccc cttcaagtgg ctcggcgacg
     1021 ccagatggct gatcaaactg gccgatatga agctctttta caccccgcag agcttcaaca
     1081 gccgcatctc cgggacgcgc accaacagcc tctcgctcac ccgcaccggt gtggaaaccg
     1141 acaacggcac ctttgcggtc aactcctcga tcggcggctc gatgaaggtc ttcgagtccc
     1201 tctccctcga cctcaaccgg acctatgcca acgacctcta cgacattccg ccggactctt
     1261 tgcgcatgat gctggaccgc ggccagctcg gctacctgac caatatgaac cagaatttca
     1321 agatcggcta taatcccaag atctttaact ggctgaccaa caatttcacc tacagcgccg
     1381 gcttccgcta cggattcaac cgtcagcaga aggtgcagtc ccgtaacgcc cagcttgcca
     1441 agaccctggg cgtgaacggt tcgctcgatc tcgagaatct ctgggcctca atctacaagc
     1501 cggccggggc cgtcaagggt ggcaagaggc cgacgcccgc ggcgcgcaga cccgcgccgg
     1561 gaaaagaggg gaccgccggc gaggagcaga aaaaagagaa ggaaaaggag aaggagaaag
     1621 gggatggctc cggcttttcg gtgatgagcc tgttcagcaa gttcttcggc tttttcgaac
     1681 ccttcagtat cacctataac cagcgtgaga acctcaccac ctacggcatc agccggatgc
     1741 cggggatgag cttcatgctc gggctgcagg acagcctggg cgtgccgatc gagagtgcgg
     1801 tgggcgcctc cggcagcagc agcagcgtca accgcaacac ctcgagcacc aatgaaaatg
     1861 tccatatctc ttcgggattt tccataactc gcaatatcaa ggtgagcctg aactacgaca
     1921 attcggcctc tctcaaccgc agcaccacga cgaccggcca gcgttcgaac agccggctca
     1981 tctacgggga ggatattgac atgcccttcc cgcagtggac cctgcgcatc agcggtctgg
     2041 aaaaactgcc cctgatcaac aaatacgtgc agaccatgag cctggaccat ggctattccg
     2101 gccagttcga ccagacctac aacatggaga acggcaagga ggtcatcacc aaggaggatc
     2161 gcaattccaa gttttcgccc ctggcgggca tgagtctgag cttcaagaac ggtatgacca
     2221 tgtcgatgcg ctacacctcc ggcgagaaca actccctctc ttcgggatac agcgtgggcg
     2281 ggaccagaac cacgaccaac gatctctcgg tctcagccca gtaccgcaag cagagcaatt
     2341 tccgcattcc catcccggtc tggcccttca agaacatgcg gctgaaaaac agcgtcaatc
     2401 ttcaggtcac gatctcgtcc aagagcaatg tcacgcgcaa gagccgcgcg ggcggggagt
     2461 atgaaattac cgccgaaacc agccaatggc tcctcaaacc ggacctccag tacagttttt
     2521 ctgagcgcgt caatggcggc atgttcctcg agctgggcaa gacccacaac aagctgatcg
     2581 gggacacctc ctactatgaa ttcgggctca atgccaatat atcgatccgc ggccgttagg
     2641 aaaacactgt cccgcgcctg gaggcggagc gctcacaccg caagagaggc cggtgacagc
     2701 cgggcgcagc ggtccggccg cctggatggg cggcctcggc ggctccggcc gggctgagag
     2761 aggagataga atgaaattat ccagacttgt catcggcgtc gccctgatca gcgccggact
     2821 ggcgctcagc tgcagcgccc agaatccgcc cccgttcgac ggggccaggg cctacaccgt
     2881 cctccagaag cagtgcgatt tcgggccgcg ggtgccgggc agcgccggcc accgcagctg
     2941 ccgcgactat ctggtcaccg ccctgcgcgg ctatgccgcc caggtgtcca cccaggagtt
     3001 cattcacacc tttggccagc ccccccaatc ggccagagcc accaatatca tcgccaattt
     3061 ccagccggaa aagggtgaac ggatcctgct ctgcgcccac tgggacagcc ggccctgggc
     3121 ggaccaggac ccggatccgg ccaaccacag caagcccatc ctcggcgcca acgacggcgc
     3181 atccggtgtg gccgtcctgc tggaaatcac ccgcctgatt cgtcaaaagc cgccccgctg
     3241 cggggtcgac atcgtccttt ttgatgcgga ggatcagggg ctggag
//
LOCUS       DAOPWU010000369         3282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_29816_length_3282_cov_4.454602, whole genome shotgun sequence.
ACCESSION   DAOPWU010000369 DAOPWU010000000
VERSION     DAOPWU010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29816_length_3282_cov_4.454602"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..2282)
                     /locus_tag="PKM23_13770"
     CDS             complement(129..2282)
                     /locus_tag="PKM23_13770"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNY92586.1"
                     /translation="MMNYSSNRKNQQRRDQSRLNLYYRVLATIVIVVVLRLVYIQIIV
                     REKYAVLAEKQYLQEVRLDPVRGTIYDRRMRPLAINVPTVSIGVYPQRISNPAATARK
                     LSRILGVSSSTLQAKMRRDRKFGYLVRREARHTGLLVEALGLKGISIIKENGRKYPMG
                     EVGSQILGFMDADGHGISGIEKKLEPLLFGVPGHAVKHKTAVGNQYSFYHPDYPVHQA
                     EPGRHVVLTIDAGIQSIAHRELRRTLQESNADSGVVVVMDPKTGEVLAMASEPGFNPN
                     AYGKFAPSSYRLRAITDQFEAGSTFKLVTMAGLLNEGIYTPQDKIFCENGVWKVMGET
                     IHDVHKYGSLSIHDVIVKSSNIGMAKAIKDQDKGIVYRYARDFGFGTRTGIGLEGEVN
                     GQLRDMAEWSGFTPYAMAFGHEIAVTPIQMANMFCTIANGGILLTPYLVKEIQDAEGH
                     VQERFRPRAVRRVIKVETANILKEMMADVVGEGTARKAQLEGLTVCGKTGTARMVRSG
                     GGGYIKGQYVANFGGFFPKENPDVAIYVMIDNPKGSYMGGDVAAPCFRRIAQQIIYQR
                     GVDVEAEEGKDLDLKFVQEDRRIVPNFVGFNRSAAVQMAGDLKIELAITGRHGVIVEQ
                     HPRAGAEISIPATVKLVCKEGPSDEVRHVVPRVTGLPIRNALNVLAAEGITAVVNGSG
                     KVVSQQPPPGRRTNPGDQVLLVCESSVDLRKLLIF"
     gene            complement(2323..2679)
                     /locus_tag="PKM23_13775"
     CDS             complement(2323..2679)
                     /locus_tag="PKM23_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92587.1"
                     /translation="MRLQSKRYQVRPHKSDYLRVREQVIAVLAIIAVLVFLKVWQKVN
                     VDHQIRRNGKLEQELLVLQGENALLEVRIDGLRSMQRMGELAESRLKLVPVPTIVLRE
                     KGVVAKLVDKLEDWQR"
     gene            complement(2691..>3282)
                     /gene="rsmH"
                     /locus_tag="PKM23_13780"
     CDS             complement(2691..>3282)
                     /gene="rsmH"
                     /locus_tag="PKM23_13780"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNY92588.1"
                     /translation="EKGFMFSADGPLAMQMDPQSGLSAETVVNEFDEGQLGEIFRTLG
                     EERAWRRIARAVVRARSRGRIIRTGQLADIIRAAVPGPHTIKSCARVFQSLRIFVNDE
                     LRNLELFLPQALSALRPGGRLGVISFHSLEDRIVKEFIRNEADPCTCPPKLPFCVCGA
                     VARLRPVGRLVVPSAAEVAANPSARSARLRVAEKIV"
BASE COUNT          640 a         1069 c          931 g          642 t
ORIGIN      
        1 gcgccaggca gttggcgtgg ttctccagcc gcaccgggcg ctgcagcagc gcctcttctc
       61 cgaaccactc gcccccagtc cagtgcagaa tatgagccgg tgtcagttct gaataggcca
      121 ttatcttctc aaaaaatgag cagttttctc agatcgaccg aggattcgca caccagcagc
      181 acctggtcgc ccggattggt ccgccttccg ggaggcggct gctgcgagac caccttgccg
      241 ctgccgttga caacagcggt gatgccctcc gcagcgagaa cattgagggc gttgcgaata
      301 ggcagtccgg tcacgcgcgg cacaacatgc cgcacctcat cggaggggcc ctccttgcag
      361 accagcttga cggttgcggg aatcgaaatc tccgccccag cccgcggatg ctgctccaca
      421 atcacgccat gacgcccggt gatggccagc tcgatcttga gatcaccggc catctgtacg
      481 gccgcggacc ggttgaaccc gacaaaattc gggacgatcc ggcggtcctc ctgaacaaat
      541 ttaagatcca aatccttgcc ctcctccgcc tccacatcaa ccccgcgctg atagatgatc
      601 tgctgcgcaa tccgcctgaa acagggcgca gcaacatccc cgcccatata gcttcccttc
      661 ggattatcga tcatcacata gatcgccaca tcgggattct ccttggggaa aaatccgccg
      721 aagttggcca catactgccc cttgatgtaa ccgccgccgc cgctgcgcac catgcgcgcc
      781 gtcccggtct tgccgcaaac cgtcagtccc tcgagttggg ccttcctggc cgtcccctct
      841 cccaccacat cggccatcat ctccttgagt atgttcgcgg tctccacctt gatcacccgg
      901 cggaccgccc tgggccggaa cctctcctgc acgtgcccct cagcatcctg aatctccttg
      961 accagatagg gggtgagcag aatcccgccg ttggcgatgg tgcagaacat gttggccatc
     1021 tggatcgggg tcaccgcaat ctcgtggccg aaggccattg cgtagggggt gaaaccggac
     1081 cactcggcca tatcgcgaag ctgcccgttg acctccccct ccagcccgat gccggtgcgg
     1141 gtgccgaagc cgaaatcacg cgcataccgg tagacaatcc ccttgtcctg gtccttgatc
     1201 gccttggcca tcccgatgtt agaggatttg acgatgacat catgaatgga aaggcttcca
     1261 tacttgtgaa cgtcgtgaat ggtctctccc atcaccttcc agaccccgtt ctcgcaaaaa
     1321 atcttgtcct gcggggtata gatgccctca ttgaggagac cggccatggt gaccagcttg
     1381 aaggtcgaac cggcctcgaa ctggtcggtg atggcgcgga ggcggtagga agagggcgcg
     1441 aacttgccgt aggcgttggg attgaatccc ggctccgaag ccatggccag cacctcgccg
     1501 gtcttgggat ccatgaccac gaccacgccg ctgtcggcgt tgctctcctg cagggtccgg
     1561 cgcagctcgc gatgagcgat gctctggatg ccggcgtcga tggtgagcac aacatgtctg
     1621 cccggctcag cctgatggac cgggtagtcg ggatggtaaa aggagtactg attgccaaca
     1681 gcggtcttgt gtttcacggc atggccgggt acgccaaaga gcagaggctc gagctttttc
     1741 tcgatgccgc tgatgccgtg gccgtcagca tccataaagc cgaggatctg gctgccgacc
     1801 tcgcccatgg gatacttgcg gccgttctcc ttgatgatgc tgatgccctt caatccaagg
     1861 gcctcgacca gcagtccggt atgccgtgct tcgcggcgca ccagatagcc gaacttgcgg
     1921 tcccggcgca ttttggcctg cagggtcgag gaggagacgc cgaggatgcg gctcagcttg
     1981 cgtgcggtgg ccgcgggatt gctgatccgc tgcggataga cgccgatcga aaccgtgggc
     2041 acattgatcg ccagcggccg catacggcgg tcgtagatgg tgccccgcac cgggtcaagc
     2101 ctgacctcct gcagatattg tttctcagcc agcacggcgt atttctcccg gacgatgatt
     2161 tggatgtaga ccagcctcaa caccacaacg atgacgatgg tggcgagcac ccggtagtag
     2221 agattcagac gcgactgatc cctgcgctgc tgattttttc gattggagga gtagttcatc
     2281 atctgtcact gtttttacgg ttccaggacc cgttgcggaa ggtcagcgct gccaatcttc
     2341 cagtttatcc accagcttgg ccaccacccc tttctcccga aggacgatgg tcggcaccgg
     2401 caccagcttg agccggcttt ccgccagctc gcccatccgc tgcatcgagc gcagtccgtc
     2461 gatcctgacc tcgagcaggg cgttctcgcc ctggaggaca agaagctcct gctccagttt
     2521 gccgttgcgc cggatctgat gatcgacatt gaccttttgc cagaccttga ggaaaaccag
     2581 cacggcgatg atggccagga ccgcgatcac ctgttcgcgt acgcgcagat agtcgctctt
     2641 gtgcggacgg acctgatatc tcttgctctg cagacgcatg gattgctcac ctagacgatt
     2701 ttttccgcca cccgcagccg cgccgaacgc gcgctcggat tggccgcaac ctccgcggcc
     2761 gaaggcacga ccagcctgcc aaccggccga agacgcgcca ccgccccgca gacacagaac
     2821 ggcagcttcg gcggacaggt gcagggatca gcctcattgc ggatgaactc cttgacgatc
     2881 cgatcttcca gagagtggaa gctgataacc ccgagacgac cgccgggccg caaggccgac
     2941 agggcctgcg gcaaaaagag ctcaagattc ctgagctcgt cattgacaaa gatacgcagg
     3001 ctttgaaaaa cccgcgcaca acttttgatc gtatgcggac ccggcacagc ggcgcgaatg
     3061 atgtcggcga gctggccggt gcggataatc ctgccccggc tgcgcgcgcg caccacagca
     3121 cgcgcaatcc gccgccaggc gcgctcctca cccagggtgc ggaagatctc gcccagctgc
     3181 ccctcgtcaa actcgttgac gaccgtctcg gcgctgagac cgctctgcgg atccatctgc
     3241 atcgcaagcg gtccatccgc tgaaaacata aaacccttct cc
//
LOCUS       DAOPWU010000370         3244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_30308_length_3244_cov_5.301035, whole genome shotgun sequence.
ACCESSION   DAOPWU010000370 DAOPWU010000000
VERSION     DAOPWU010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30308_length_3244_cov_5.301035"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKM23_13785"
     CDS             <1..366
                     /locus_tag="PKM23_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin"
                     /protein_id="HNY92589.1"
                     /translation="LHSGIFYAKTINPTNLKRQAGKSLEEIRIVPNPYSIRARDLQFG
                     DSGPDRIMFYNIPGVCTIKIFTERGDLIETIEHTNGSGDEAWNSITSSAQTVVSGLYI
                     VVFETPDGQKAIRKFVVIR"
     gene            413..1423
                     /locus_tag="PKM23_13790"
     CDS             413..1423
                     /locus_tag="PKM23_13790"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY92590.1"
                     /translation="MKRMIIFVVAVLFVLGALSTDGLAQKKLAQTGCQFLSVGTDARA
                     TGMGEAFTTVEGAATALFYNPAGMAGELKNVNASFTQMTWIADIEYLAISLAGRPFRG
                     QYGTLGVSAMAIDYGELDGTIVSESEEQGYLDTGKFGPSAWTVGLGYAIDLTDRFSVG
                     GQAKYVHQDLGSAVIPLNNEPPYITDKKDYTQNVFAFDFGTLYKTGFKSLAFGMCVRN
                     FSREVKFEREGFQLPLTFKVGFSMDVLDFFPELALNHNLLLSVDAVHPRDYPEYLNIG
                     AEYKLLDLFALRAGYITGQDDYGFTAGFGVDTNFGVGIDYSYAPMDVFNNIHRFTCRL
                     GL"
     gene            1462..2685
                     /locus_tag="PKM23_13795"
     CDS             1462..2685
                     /locus_tag="PKM23_13795"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNY92591.1"
                     /translation="MAKRLILFQVIILAAALMIVLAGCDKDEYPPSLYDPDYVSKGAA
                     VISSVNPSALALSGVTEIVINGTNFSPVKENNLVFFNAEKGTVLEATESMLKVRAPIY
                     ASDSVALRIAVQGAVLFSNIWPYKLEPAQEPLVKFTTAEVPYAVATDAEGNVYTSMVL
                     SNAGAGVKKIAAGSDKFVDFAPKGGETYYSAMKIGPGNTLYGVRNVKGIFTYKAGDAK
                     PAVFASSGLGNIIDIDFDANKNVWGVGNNTDVYRVKPDKSVAKFAFKANLRAVRVYNN
                     YLYVAGDIDKVVKVWRFPINGDELGAVEEVFNFTDKVGANFAIYCITFSADGYMYIGT
                     DAPAAIYLLTPGGALEALYPGQFVPKVISFAWGLGTDLFMTREVTIEAEKVKYQQLIF
                     RINTQKEGAPQFGIL"
     gene            2877..>3244
                     /locus_tag="PKM23_13800"
     CDS             2877..>3244
                     /locus_tag="PKM23_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92592.1"
                     /translation="MKKVLTVLAVLVFMFAVSSFAGWQYVGPFPADTTALTGWPYSAS
                     THGLAVDPEGKVWVHQYYAFTRDSVLVPISTRPSKHEYVRCIYVYNRDGSPASFSPIK
                     FITVDGVVDTVGGFTDTSVSP"
BASE COUNT          684 a         1002 c          878 g          680 t
ORIGIN      
        1 ctgcacagcg gcatctttta cgccaagact atcaacccca ccaacctcaa gcgtcaggcg
       61 ggcaagagcc tggaggagat ccgcatcgtc cccaatccct acagcatccg cgcccgggat
      121 ctgcagttcg gcgacagcgg tccggaccgg atcatgttct acaacatccc tggggtttgc
      181 acgatcaaga tcttcacgga gcggggggac ctgattgaaa ccatcgaaca taccaacggc
      241 agcggcgacg aggcctggaa ctccatcacc tcctccgccc agaccgtcgt cagcgggctc
      301 tacatcgtcg tttttgaaac ccctgatggc caaaaagcca tccgcaaatt tgtcgtcatc
      361 cggtaaaggc caaggctgtt gagacgaaat caagataaag gagtaatgga ttatgaagcg
      421 tatgatcatc tttgtcgtag ccgttctttt cgtgctcggc gccttgtcca cagatggcct
      481 ggcacagaaa aagctcgccc agaccggatg ccagttcctc agcgtcggca ctgacgcccg
      541 ggcgaccggt atgggagagg cgtttacgac ggtggaaggt gcggcgaccg ccctcttcta
      601 caatcccgcc ggcatggcgg gtgagctcaa gaatgtcaat gcctctttca cccagatgac
      661 ttggatcgcc gacatcgagt acctcgccat ctcgctggcc ggccgtccct tccggggcca
      721 gtacggcacc ttaggcgtca gcgccatggc catcgattat ggtgagctgg acggcacgat
      781 cgtgtcggag agcgaagagc agggctatct cgataccggc aaattcggcc cgagcgcctg
      841 gacagtcggc ctcggttatg ccatcgacct caccgaccgt ttctcggtcg gcggccaggc
      901 caaatatgtc catcaggatc tgggcagcgc tgtcatcccc ctcaacaacg agccgcccta
      961 catcaccgac aagaaggatt acacccagaa cgtcttcgcc ttcgacttcg gcaccctcta
     1021 caagaccggc ttcaagagcc tggccttcgg catgtgcgtg cgcaactttt cacgcgaggt
     1081 caagttcgaa cgcgagggat tccagcttcc cctgaccttc aaggtgggct tttcgatgga
     1141 cgtcctcgac ttcttccccg agctcgccct taatcataac ctgctccttt cggtggatgc
     1201 agtccatccc cgcgactatc ccgagtatct caacatcggt gccgagtaca agctgctgga
     1261 tctcttcgcc ctgcgcgccg gctacatcac ggggcaggac gactatggct tcacagccgg
     1321 cttcggcgtc gacaccaact ttggcgtggg catcgattac tcctacgccc cgatggatgt
     1381 tttcaacaac atccaccgct tcacctgcag actcggcctg taattcactg aacttgtatc
     1441 cttaaattga ggacgtccca tatggcaaag cgattgatct tatttcaggt gatcatcctg
     1501 gcggctgccc tgatgatcgt actggcaggg tgcgacaagg atgaatatcc gcccagtctc
     1561 tacgatccgg actatgtgag caagggggcg gcggtgatca gctccgtcaa tccctccgcc
     1621 ctggccctct ccggggtgac ggagatcgtc atcaacggca ccaatttttc tcccgtgaag
     1681 gaaaacaacc tggtcttctt caacgcggag aaggggaccg tgctcgaggc cacggagagc
     1741 atgctcaagg tgcgggcgcc gatctatgcc tcggattccg tggccctgcg catcgcagtt
     1801 cagggcgccg tgctcttcag caacatctgg ccttataaac tggagccggc ccaggaaccc
     1861 ctggtcaaat tcaccaccgc cgaggtgccc tacgccgtcg ccaccgatgc cgaaggcaat
     1921 gtctacacct cgatggtgct cagcaatgcc ggcgccggcg tcaaaaagat cgccgccggc
     1981 agcgacaagt ttgtcgattt tgcgcccaag ggcggcgaga cctactattc ggcgatgaag
     2041 atcgggccgg gcaacaccct ctacggcgtc cgcaacgtca agggtatttt cacctacaag
     2101 gcgggtgatg ccaaaccggc ggttttcgct tcttccggcc tgggcaacat catcgacatc
     2161 gatttcgacg ccaacaagaa tgtctggggc gtcggcaaca acaccgatgt ctaccgcgtc
     2221 aagccggaca aatcggtcgc caagttcgct ttcaaggcca acctgcgggc ggtccgcgtc
     2281 tacaacaact atctctatgt ggccggcgat atcgacaagg tcgtcaaggt ctggcgcttc
     2341 cccatcaacg gtgatgagtt gggcgcggtc gaagaggtgt tcaattttac cgacaaggtc
     2401 ggcgccaatt tcgccatcta ctgcatcacc ttctcggcgg acggctatat gtatatcggc
     2461 accgatgccc cggcggctat ttacctgctc actccgggcg gagcccttga ggccctctat
     2521 cccggccagt ttgtccccaa ggtcatcagc tttgcctggg ggctcggcac cgatctcttc
     2581 atgacccgtg aagtgaccat cgaggctgaa aaggtcaagt accagcagct catcttccgc
     2641 atcaacaccc agaaagaggg cgcgccgcag ttcggaattc tctagattga atcaacccgg
     2701 cctcgcccac ccgaatcagg ggaggtgtgg ggccggaaca gaccacgcaa tcggttttgc
     2761 acgacaacag atgcagcgca aagagtgaaa atcgaaagga ggtggggcaa agggcattga
     2821 caaccgcttc agaagagtaa attctcgttt ttaaaccaat tcatttggag gacaacatga
     2881 agaaagttct aacagttctg gcagttctgg tgtttatgtt tgctgtcagt tccttcgctg
     2941 gttggcagta tgtcggtcct ttccccgccg acacgactgc tctgacgggc tggccctaca
     3001 gcgcctcgac ccacggcctc gccgtcgatc ccgagggcaa ggtttgggtg catcaatact
     3061 atgccttcac tcgtgacagc gtgctggttc cgatcagcac ccgtccgagc aagcatgagt
     3121 atgtgcgctg catctatgtg tacaatcgcg acggctcccc ggcctccttt tcgccgatca
     3181 agttcatcac cgtggatggc gtggtcgata ccgtgggcgg cttcaccgac accagcgtct
     3241 cccc
//
LOCUS       DAOPWU010000371         3222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_30604_length_3222_cov_2.834228, whole genome shotgun sequence.
ACCESSION   DAOPWU010000371 DAOPWU010000000
VERSION     DAOPWU010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30604_length_3222_cov_2.834228"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKM23_13805"
     CDS             <1..470
                     /locus_tag="PKM23_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92593.1"
                     /translation="ADLWDGENRERAAHTVMLGMTGVGLAGGYLLSQREHYTRGDAYV
                     LRMEGILGAQIMMPIAAAIADKKEKSYTVGAMTGGVLGVGIGNWLLKDEDFDFNEGAL
                     ISCGQIAGGLLAGGLTYLIDTNENFDGLVYHSAIALGSLAGHAFLYHTFKLRR"
     gene            complement(589..2307)
                     /locus_tag="PKM23_13810"
     CDS             complement(589..2307)
                     /locus_tag="PKM23_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNY92594.1"
                     /translation="MFLPATRQEMNELGWEACDVILVTGDSYIDSPFNGAAQIGKWLH
                     HHGFRVGIIAQPSVENPEEITRLGEPGLFWGVSGGCVDSMVANYTALKKRRKGDDFSP
                     GGLNNRRPDRAVIVYTNLIRRSFKATRPIVLGGIEASLRRIAHFDYWSDSIRRSILFD
                     AKADALIYGMGERAVLELAQALAQGGDFREIRGLCYIAHAPRPDYLTLPDFETVAADQ
                     EAFTGMFRLFYDNNDPVTARGLNQQHGDRWLVQNPPALHLSEAELDQAAELPFEREQH
                     PWHQQFGAVRALDTIRFSINTHRGCYGECHFCAIAVHQGRTVLSRSEASILREAEELT
                     RHPAFRGIISDAGGPTANMYGFECGRKKSRGSCQFKACIGAQVCSTLRPDHRRQTELL
                     KKLRRLPGVKKVFVASGLRYDLILEDRTGGEAYLRELTAHHVSGQLKVAPEHTEPHVL
                     NLMNKPAAASLLEFKRRFDRLSAEAGKNQFLTYYLIAAYPGCTDLDMQAMQRFVSSEL
                     RITPEQVQIFTPTPSTWASVMYYTEKNPFTGERLFVEKNGAAKERQKQRIVGGVARKK
                     TGRKGG"
     gene            2538..>3222
                     /locus_tag="PKM23_13815"
     CDS             2538..>3222
                     /locus_tag="PKM23_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/glucose cotransporter"
                     /protein_id="HNY92595.1"
                     /translation="MSVIAGLDWVIIFAYFVIILGLAWWVIRRQKQTTDGYFLAGRSL
                     GWFIVGASIFASNIGSEHLVGLAGSGATDGVAMAHYELHAWCLLVLGWVMVPFYMRSK
                     VYTMPEFLERRFSPSARTFLSVVSLVAYVLTKIAVGIFAGGVVFSVLLPELNWFGLDS
                     FWIGSVLVILITGLYTILGGMKAVAYTEALQTVVLVIGSALVTYFGLKALGGWGELRV
                     IAGSEMFNLW"
BASE COUNT          556 a          934 c         1038 g          694 t
ORIGIN      
        1 tcgcggattt atgggacggg gagaaccgcg aacgggcggc tcatacggtc atgctcggga
       61 tgacgggagt gggactggcc gggggatacc tgctttcaca gcgcgagcac tatacccgcg
      121 gcgacgccta tgtgctgagg atggagggga ttcttggtgc gcagatcatg atgcccatcg
      181 ctgcggccat cgccgacaag aaagaaaagt cctatacggt cggcgccatg accggcgggg
      241 tcctgggtgt cgggatcggc aactggctgc tgaaggacga ggattttgat ttcaacgaag
      301 gggcgctgat ctcctgcggc cagatcgccg gcggattact ggcggggggt ctgacctatc
      361 tgatcgatac caacgaaaat tttgacggcc tggtctacca ttcggccatc gctctgggca
      421 gcctcgccgg ccacgctttt ctctaccata cgttcaagct gagacggtga ggcggcgcgc
      481 cagcgctgac gatccggccc atgcgtgtgt tatagtttcc gcgtcggctc cccgggaagg
      541 cggctgcacg ccggcaggga gccatgcgcc tgtgacactt ttccggcctc aaccaccctt
      601 cctgcccgtt tttttccggg ccactccccc cacaatccgc tgtttctgcc gctctttggc
      661 tgctccgttc ttttcgacga agagcctctc gccggtgaag ggatttttct ccgtataata
      721 catgacgctg gcccaggtgg atggcgtcgg cgtaaagatc tgcacctgct cgggggtgat
      781 ccgcagctcg ctgctgacaa agcgctgcat cgcctgcatg tcgaggtcgg tgcagccggg
      841 ataggccgcg atcaggtaat aggtcaggaa ctggtttttg cccgcctcgg ccgagagccg
      901 gtcgaagcgc cgtttgaact ccagcagcga ggcggccgcc ggtttgttca tcaggttgag
      961 cacgtgcggt tcggtatgct ccggggccac cttgagctgt ccggagacat ggtgagccgt
     1021 caactcccgc agataggctt cacctccggt ccggtcttcc aaaatcagat catagcgcag
     1081 tcccgaagcg acaaagacct ttttaacgcc cggcagccgg cgcaattttt tgagcagttc
     1141 ggtctggcgc cggtgatcgg gcctgagggt ggagcagacc tgtgcgccga tgcaggcttt
     1201 gaactggcaa ctccccctgc tttttttgcg gccgcattcg aaaccataca tattggccgt
     1261 cggcccgccg gcatcgctga tgatgccgcg gaaggcgggg tggcgggtca gttcctccgc
     1321 ctccctgagg attgaggctt cgctgcggga gaggacggtt cgcccctggt ggacggcgat
     1381 ggcgcaaaaa tggcattcgc cgtaacagcc gcggtgggtg ttgatggaaa agcggatcgt
     1441 atcgagagcg cgcaccgctc cgaactgctg atgccaggga tgctgttcgc gctcaaaggg
     1501 gagttcagcg gcctggtcca gttcggcttc gctgaggtgg agggctggcg gattctgcac
     1561 cagccagcgg tcgccgtgct gctgattgag gccgcgggcc gtcacgggat cgttgttgtc
     1621 gtaaaataga cggaacatcc cggtaaaggc ctcctggtcg gcggcgacgg tctcaaagtc
     1681 cggcagggtg agatagtccg gacggggcgc atgggcgata tagcagagcc cgcggatttc
     1741 ccggaaatca ccgccctggg ccagtgcctg cgcgagctcc aggacggccc gttcgcccat
     1801 gccgtagatg agggcgtcgg ccttggcgtc gaagaggatg gaacggcgga tggaatcgga
     1861 ccaatagtca aagtgggcaa tgcggcgcag gctggcttcg atgccgccga ggacgatcgg
     1921 ccgggtggct ttgaacgagc gccggatcag gttggtataa acgatcacgg cccggtcggg
     1981 gcggcggttg ttgagcccgc ccggggaaaa atcgtcgccc ttccgccgct tcttgagggc
     2041 ggtatagttg gcgaccatcg aatcgacaca gcctccgctg acgccccaga agagcccggg
     2101 ctcgccgagg cgggtgatct cctccgggtt ctcgaccgat ggctgggcga tgatgccgac
     2161 gcggaagccg tgatggtgga gccatttgcc gatctgcgcg gcgccgttga aggggctgtc
     2221 gatgtaactg tcaccggtga cgaggataac gtcgcacgcc tcccagccca gctcgttcat
     2281 ctcctgccgt gttgcgggta aaaacatggt ttcctctcct ggctgccgtc caatatagtg
     2341 aaatcggcga cagaatgcat gcaaaattat cgctttacct gccgccattt tcgctgagat
     2401 tatttttgca ttgaacacaa ttaattgcta tatttcccga aacgtgaaga gaacttcgcc
     2461 gggccccgcg ggggcgatcc aggagatcag gtgacaaaac catagcaagc ccctaatcaa
     2521 cgagagagga gcagatcatg tcagttattg cgggtctgga ttgggtcatc attttcgcct
     2581 actttgttat tattctcggt ctggcctggt gggtgatccg gcgccagaag cagacaacgg
     2641 acggctattt tctcgccggg cgctcgctgg gatggttcat cgtcggcgcc tcgattttcg
     2701 cctccaacat cggctccgag catctggtcg gcctggccgg ctccggcgcc accgacggtg
     2761 tggctatggc ccattatgag ctgcacgcct ggtgtctgct ggtgctgggg tgggtgatgg
     2821 tgccctttta tatgcgctcc aaggtctaca ccatgcccga gtttctcgag cggcgcttct
     2881 cgccgtcggc ccggaccttc ctctcggtcg tctccctggt cgcctatgta ctgaccaaga
     2941 ttgcggtcgg catcttcgcc ggcggtgtgg tctttagcgt gctcctgcct gagttgaact
     3001 ggttcggcct cgacagcttc tggatcggat cggtgctggt catcctgatc accgggctct
     3061 ataccatcct gggcggcatg aaagccgtcg cctataccga agccctgcag acggttgtcc
     3121 tcgtcatcgg ctcggccctg gtgacctact tcggtctcaa ggccctcggc ggctggggcg
     3181 agctccgtgt catcgccggt tccgagatgt tcaacctctg ga
//
LOCUS       DAOPWU010000372         3219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_30639_length_3219_cov_3.905183, whole genome shotgun sequence.
ACCESSION   DAOPWU010000372 DAOPWU010000000
VERSION     DAOPWU010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30639_length_3219_cov_3.905183"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKM23_13820"
     CDS             complement(<1..267)
                     /locus_tag="PKM23_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY92596.1"
                     /translation="MNDIIIACEHLCVAYQDKLALENITFNIHHGQFWGILGPNGSGK
                     TTLLRTMLGLIEPLSGTVSLFGRAPRQLGALRDKIGYVPQYAQID"
     gene            complement(275..1156)
                     /locus_tag="PKM23_13825"
     CDS             complement(275..1156)
                     /locus_tag="PKM23_13825"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNY92597.1"
                     /translation="MKRIALLLLAAFITVAGCGKSPQSQGKVIVAASIPPLADFVRQV
                     GQNKVEVVNIVAGAANPHTFEITPETIRRASRARMLVLNGLGLEFWAPKLVDAIGRNS
                     LKVVDTSQGIQPIKDEEHDHDHGGTNPHIWLSPPLAIHQVKAIRDALVEIDPGNASHY
                     RRSSQAYIDSLLALDNEITLEIAGWLQRSFICFHPSWNYFADNYKLNQAAVIEKRSGF
                     EPTPREIVEIIELVKKLDISTIFAERQFPTQSSETIARECGAKVISLDPLGEESPAFS
                     YLSLMRLNVAKMAQAMK"
     gene            complement(1187..1975)
                     /locus_tag="PKM23_13830"
     CDS             complement(1187..1975)
                     /locus_tag="PKM23_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNY92598.1"
                     /translation="MKLAQQTILITGASSGIGAELARQLADRGCRLLLTARRGALLDE
                     LIAGLPGGSQRHCAFSCDLSCPEEVESLCRTILGEHAQLDGMILNAGVGGGFNAAAID
                     VAGLRYQFEVNFWGAVGFIARLSPLLLERRKGFIAVTSSLAGYRGLPAAAAYSASKAA
                     LTRFIESMRIDCRRSGVHFAVISPGFVRTPMTERGEGLRPFMIDVPRAAAIIIRGLER
                     RRYEIRFPWLMVLLVRFGLILPEGLYMKLLAGRRKPQPTPGAGS"
     gene            2237..>3219
                     /locus_tag="PKM23_13835"
     CDS             2237..>3219
                     /locus_tag="PKM23_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92599.1"
                     /translation="MSGRKLATGLLIWLITVSAVQAQVIIQAQVLQRDLRLFFLSDLN
                     LTGRGRATNEVFSISIINSAAIQQICCLHMDLSKQEGGRSQLLASGTTNPFTLAAGRT
                     IRITNVNLFSQAQQFSLDDYAIEDSGEEVQSRILATGKLPTGLYRFTFRIRPCSGSGF
                     SPSETYIEFDVSNPTSLDLIGPGEPADRALPGLSPTLFPLFRWNSNLSRFKLTVAEKL
                     AGVHDAASPAEIIQDRIRFERLFTVDPARSGGIAPDGSEYISGTSYPYPSAGAWPLEE
                     GKTYYWRITGVAPSSGAEAEFPSEIWAFRTDAGAMHSLEIRAGMSEMLASLR"
BASE COUNT          639 a          927 c         1005 g          648 t
ORIGIN      
        1 atcgatctgg gcgtactgcg gaacgtagcc gattttgtca cgcagcgcgc cgagctgacg
       61 cggggcacgg ccgaagaggc tcacggtgcc gctcaggggc tcgatgaggc cgagcatggt
      121 gcgcagcaag gtggtcttgc ccgaaccatt gggtccgagg ataccccaga actggccgtg
      181 gtggatatta aaggtgatgt tttcgagcgc gagcttgtcc tgataggcca cgcagaggtg
      241 ctcgcaggca atgatgatgt cgttcatgca gggctcactt catggcctgg gccatttttg
      301 cgacattcag gcgcatgagg ctgaggtagg aaaaggcggg gctctcctcc ccaaggggat
      361 cgaggctgat cactttggcg ccgcactccc tggcgatggt ctcgctgctc tgggttggga
      421 actgccgctc ggcgaagatg gtggagatat ccagtttttt aaccagctcg atgatctcga
      481 cgatttcacg cggagtcggc tcaaagccgg agcgtttttc aattacggcc gcctggttga
      541 gcttgtagtt gtcggcgaag taattccagg agggatgaaa gcagatgaat gacctttgca
      601 gccagccggc aatctcgagg gtgatttcgt tgtcaagggc aagcagggag tcgatatagg
      661 cctggctgct acggcggtag tgtgatgcat tgccgggatc gatttcgacc agggcatcgc
      721 ggatggcttt tacctgatgg atggccagtg ggggtgaaag ccagatgtgg ggattggttc
      781 cgccatggtc atgatcatgc tcctcgtctt taatgggctg gatgccttga ctggtatcga
      841 ccaccttgag ggagttcctg ccgatggcat ccaccagctt gggggcccag aactccagtc
      901 ccaggccgtt gaggacgagc atgcgggcac ggctggcccg gcggatggtt tcaggggtaa
      961 tctcaaaggt gtgcgggttg gccgcaccgg cgacgatgtt caccacttcc accttattct
     1021 gacctacctg ccggacgaaa tcggcgaggg gcgggatgct ggcggcgacg atcaccttgc
     1081 cctggctctg tggagattta ccgcagcccg cgacagtgat gaaggcggcg agcagcagga
     1141 gtgcgattct tttcataaaa gaggtatcca ttttgaagtt tcagggtcag ctcccggccc
     1201 ccggggttgg ctgcggcttg cgcctgccag cgagcagctt catgtaaagg ccctcaggga
     1261 ggatgagacc gaagcggacg agcaggacca tgagccaggg aaaacggatt tcataacgac
     1321 gcctctcaag accccggatg atgatcgcgg cggcccgggg tacgtctatc atgaagggcc
     1381 gcaagccctc gccccgctcg gtcatggggg tgcgtacaaa gccgggcgag atgacggcga
     1441 aatggacgcc gctcctccgg caatcgatgc gcatgctctc gatgaagcgg gtcagggccg
     1501 ctttggaggc tgaataggca gccgcggcgg gcagaccacg atagccggcg aggctggagg
     1561 tgacggcgat gaagcccttc cgccgctcca gcagcagggg cgagagccgg gcgatgaacc
     1621 cgaccgcacc ccagaaattg acttcaaact gatagcgaag cccggctaca tcgatggcgg
     1681 cggcgttaaa gcctccgccc acgccggcat tgaggatcat cccgtccagt tgcgcgtgct
     1741 cccccagaat ggtccggcac agggattcga cctcttccgg acaggagaga tcgcaagaga
     1801 aggcgcaatg gcgctgcgag cctcccggca gcccggcgat cagctcatcg aggagggcac
     1861 cgcgccgggc ggtcagcagc agacggcagc ctcggtctgc gagctggcgg gccagttcgg
     1921 ccccgattcc ggaggaggcc ccggtgatga ggatggtctg ttgagccagt ttcatgggta
     1981 tgggtgatat taaataaaag tgggccgccg ggtgttgatt aatgtatgca tttagacctg
     2041 aaaatgcaag accattccat gccgacggaa gctgctgtca cccgctgaga cactattcca
     2101 tctatgtaca acccggcatg accaggccgg aggacatgtt tataacacat taattaataa
     2161 cagctcgtct cttcttttcg atggcattca atttgcaata attatgcgat gggtattttt
     2221 atcctcagga ggaaccatgt ctggacgaaa actggccaca ggcttgctga tctggctgat
     2281 caccgtttcg gcggtgcagg cccaggtgat catccaggcc caggtcctgc agcgggatct
     2341 tcgcctcttt ttcctcagcg acctgaacct caccggacgc ggcagggcga ccaatgaggt
     2401 tttcagcatc tccatcatca attcggccgc aatccagcag atctgctgcc tgcacatgga
     2461 tctcagcaaa caggagggcg gccggtcgca gctcctggcg agcgggacga ccaatccctt
     2521 caccctcgcc gccggccgga ccatccgcat caccaacgtc aacctcttct cgcaggccca
     2581 gcagttctcg ctcgatgact atgccatcga ggacagcggt gaggaggtgc agtccagaat
     2641 cctcgccacc ggcaagctcc ccacggggct ttaccgcttt actttccgga tcagaccctg
     2701 ttccggcagt ggtttttccc cctcggagac ctatatcgag ttcgatgtct ccaatccgac
     2761 cagccttgat ctgattggtc cgggagaacc cgccgaccgc gcactgccgg gtctctcccc
     2821 gacgctcttc cccctctttc gctggaactc caatctcagc cgttttaaac tgactgttgc
     2881 tgaaaagttg gccggggtgc acgatgcggc cagtcccgct gaaatcattc aggaccgcat
     2941 ccgtttcgag cgcctcttca ccgtcgaccc cgccaggagc ggaggcatcg cgcccgacgg
     3001 atccgagtat atctccggca caagctatcc ctatccctcc gccggggcct ggcccctcga
     3061 ggagggcaag acctattact ggcggattac gggagtggcg cccagctcgg gggccgaggc
     3121 cgagtttccg agtgagatct gggctttcag gacggatgcc ggcgcaatgc acagcctgga
     3181 gatccgtgcc ggtatgagcg aaatgctcgc cagcctgcg
//
LOCUS       DAOPWU010000373         3219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_30647_length_3219_cov_3.127370, whole genome shotgun sequence.
ACCESSION   DAOPWU010000373 DAOPWU010000000
VERSION     DAOPWU010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30647_length_3219_cov_3.127370"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PKM23_13840"
     CDS             <1..461
                     /locus_tag="PKM23_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNY92600.1"
                     /translation="QMVIVDIAPKSYYHPFFERVLDFMQALDLGQFMTRAAIEEAFVE
                     NIPNPAVRLFILKNLQRTEEGFRWRINVQSLRDNLGEIFSAISSGRRFEKPTLFVRGG
                     RSDYILDEDEPAIRALFPAAELITIPEGSHWLHVDAEEALCRHLREYLQC"
     gene            487..1932
                     /locus_tag="PKM23_13845"
     CDS             487..1932
                     /locus_tag="PKM23_13845"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNY92601.1"
                     /translation="MSRKGEWFTIASLALALACAQAGRVQREEYRTMIDQGEFTRAGL
                     LIAERLKEPGLSPAERAGLEFEIERMARIRKDFVRNEAEVVEFIKIYIPDVDSARLAA
                     WEAEKSLEMMRIDGEKRYFKYAARNLFRINKACREIWRAAHPLDAGAAGGPGSRALDE
                     NIARILAQATPAQPLVEPVRMRIEQTLTVNADAVPAGETIRCWLPFPREIPGRQTDIR
                     LIGTTPEQHHLADTSRLQRTICFEQSAVAGTPARFKVTYEYTSLGSFTSIDPEKVTPA
                     AAEGPLQPWLREEPPHIVFTPELRALAAEVAGGETNPYRIAQKLYAWVEENIPWASAR
                     EYSTIRNLPMYAWENRHGDCGIQHLTFITLCRISGIPARWQSGWEFKPPTDSMHDWSM
                     IWFAPYGWVPADVTYGRRDAEDPVQHWFYLSGMDSYRLIFNDDVSRPFDPPKEHFRSE
                     TLDSQRGELEWRGGNLYFDQWDWELKWEVLP"
     gene            complement(1978..>2174)
                     /locus_tag="PKM23_13850"
     CDS             complement(1978..>2174)
                     /locus_tag="PKM23_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNY92602.1"
                     /translation="PLTIRCRILEQKRRFVFVESELLNPAGEICTRGKTRYFTFEREN
                     APEHLRFPDKEAFFEQEETL"
     assembly_gap    2175..2184
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2185..2473)
                     /locus_tag="PKM23_13855"
     CDS             complement(<2185..2473)
                     /locus_tag="PKM23_13855"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNY92603.1"
                     /translation="MRKIRNPFSTSPGYHCFGCCPTNPIGLQMEFWLDGGDLVAIWQP
                     KPEYQGWGNILHGGIQTTLLDEIGGWIILVALEKAGVTAQMELAFLKPVSIL"
     gene            2634..>3219
                     /locus_tag="PKM23_13860"
     CDS             2634..>3219
                     /locus_tag="PKM23_13860"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNY92604.1"
                     /translation="MAEPLVANPLQPNRLMSLDFFRGLTMFLLIGESTRLYEILRGAA
                     FNGTFLGFIGWQLEHHDWNGLHFWDLVQPFFMFIVGVAIPFSVAKRRTAGAGWPQLYR
                     HALRRSLLLLILGIGLYCISAGRLNFELWNVLAQLSFTYLVAFLLMRYPAAVQIGASL
                     GLILLSEALYRLWAVPGFDQPFVPDRNFGSWVDML"
BASE COUNT          583 a         1010 c          968 g          648 t
ORIGIN      
        1 aacagatggt gatcgtggac attgccccca aatcctatta ccatcccttc ttcgaacggg
       61 tgctcgattt tatgcaggct ctggatctgg gacagttcat gacgcgcgca gcgatcgagg
      121 aggcctttgt cgagaacatc cccaatccgg cagtgcggct tttcattctc aaaaatctgc
      181 agcgcacgga ggagggcttt cgctggcgga tcaatgtaca aagcctgcgg gataatctcg
      241 gagagatttt ttccgccatc agctccggcc gccggtttga aaagcccacc ctttttgtcc
      301 gcggcggccg ttcagattat atcctggacg aggacgaacc ggccatccgc gccttgttcc
      361 cggcggcgga gttgatcacc atcccggagg gctcgcattg gctgcacgtc gatgccgagg
      421 aggccctgtg ccgccatttg cgcgaatatt tgcagtgttg aaattctatc aggaggaggg
      481 cagacgatgt cgagaaaagg agaatggttt actatcgcca gcctcgcact ggcgttggcc
      541 tgtgcccagg ccggccgggt gcagcgggag gagtatcgca ccatgatcga tcagggagaa
      601 ttcacccggg ccggccttct gattgcggag cggctcaagg agccgggtct ctccccggcg
      661 gaacgcgccg gcctcgaatt cgagatcgaa cgcatggcgc gtatccgtaa ggactttgtc
      721 aggaacgaag ccgaagtggt ggagtttatc aagatttaca ttcctgatgt cgacagcgcc
      781 cggctggcgg cctgggaggc ggaaaaatcc ctcgagatga tgcgcatcga cggcgagaag
      841 cgctatttca aatatgccgc ccgcaatctt ttccgcatta acaaggcgtg ccgggagatc
      901 tggcgggcgg cccatcccct cgatgccggt gcagccggcg ggccgggcag ccgggcgctc
      961 gacgaaaaca tcgcgcgcat tctcgcccag gcgacacccg cgcagcccct ggtcgagccg
     1021 gtgcgaatgc gcatcgagca gaccctgacc gtgaatgcgg acgccgtccc ggccggagag
     1081 accattcgct gctggctccc ctttccgcgc gagatccccg gccgccagac cgatatccgc
     1141 ctgatcggca caacgccgga acagcatcat ctcgccgaca cctcccggct gcagcggacg
     1201 atctgtttcg agcagtccgc ggtcgccggc accccggcgc gctttaaggt cacttatgag
     1261 tacaccagcc tcggctcctt caccagcatc gacccggaga aggtgacgcc cgcggcggcg
     1321 gagggcccgc tgcagccctg gctgcgcgaa gagccgcccc acatcgtctt caccccggag
     1381 ctgcgcgccc tggcggcgga agtggccggc ggcgagacca atccctaccg cattgcgcaa
     1441 aaactctacg cctgggtgga ggagaatatc ccctgggcct cggcgcggga gtactccacc
     1501 atccgcaatc tgcccatgta cgcctgggag aaccgccatg gcgactgcgg catccagcac
     1561 ctcaccttta tcaccctctg ccgcatcagc ggcattccgg cgcgctggca gtccggctgg
     1621 gaattcaagc cccccaccga cagcatgcac gactggagca tgatctggtt cgccccctat
     1681 ggctgggtgc cggccgatgt cacctacggc cggcgcgatg ccgaagaccc ggtccagcac
     1741 tggttctatc tcagcggcat ggacagctac cggctgatct tcaacgacga cgtcagccgg
     1801 cccttcgatc cgcccaagga acatttccgt tccgagaccc tcgattcgca gcgcggcgag
     1861 ttggagtggc gcggcggcaa cctctatttc gatcaatggg attgggagtt aaagtgggag
     1921 gtgcttccgt agcgcagctt tgacgcaaaa gaggtcccgg ctgccgggac ctccctgtta
     1981 cagcgtctct tcctgctcga aaaaggcctc tttgtccgga aaacgcaggt gttcaggggc
     2041 gttttcgcgc tcaaaggtga agtagcgcgt ctttccccgg gtgcagatct cgccggccgg
     2101 attgagcagt tcgctctcca cgaagacgaa gcggcgcttc tgttcgagaa tgcggcagcg
     2161 gatggtcagc gggcnnnnnn nnnnccagga tcgagaccgg cttgaggaaa gccagctcca
     2221 tctgtgcggt cacacccgcc ttttccaggg cgaccaggat gatccagccg ccaatctcat
     2281 cgagaagggt ggtctggatg ccgccgtgga ggatgttgcc ccagccctga tactccggtt
     2341 tcggctgcca gatggcgacc agatcgccgc catccagcca gaactccatc tgcaggccga
     2401 tgggattggt cgggcagcag ccgaagcagt ggtagccggg tgaggtcgag aagggattgc
     2461 gaatcttgcg catgggggct cctgttttca taaagtaaca agtttgcagc tccaaagcaa
     2521 tcttttttgg ccggagtgcc ccgatcttcc gctccctccg gatgaggctg cccgatctgg
     2581 tgtccaacct cgccgtgctg taaaagcatc cagccggaaa gaaaggagaa cccatggctg
     2641 aacccctggt tgcgaatcct ctgcagccca atcgcctgat gtcgctggat ttcttccgcg
     2701 gcttgaccat gtttctttta atcggggaat ccacccggct ttacgagatc ctgcgcgggg
     2761 ctgctttcaa cggcaccttt ctcggcttta tcggctggca gcttgagcat cacgactgga
     2821 acggcctcca tttctgggac ctggtgcagc ccttttttat gttcatcgtc ggcgtggcga
     2881 tacctttttc ggtcgccaag cgccggaccg ccggcgcggg ctggccgcag ttgtaccgtc
     2941 acgccctgcg gcgctcgcta ctgctgctga ttctcggcat cggcctctac tgcatcagcg
     3001 ccggccgcct gaactttgag ctgtggaatg tcctggcgca gctctccttc acctatctgg
     3061 tggcctttct gctgatgcgc taccccgctg cggttcagat cggtgccagc ctgggcttga
     3121 tccttctgag cgaagccctc tatcgactgt gggcggtgcc gggattcgac cagccctttg
     3181 tgccggaccg caatttcggc tcctgggtcg acatgctgc
//
LOCUS       DAOPWU010000374         3214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_30710_length_3214_cov_2.994619, whole genome shotgun sequence.
ACCESSION   DAOPWU010000374 DAOPWU010000000
VERSION     DAOPWU010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30710_length_3214_cov_2.994619"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PKM23_13865"
     CDS             complement(<1..1002)
                     /locus_tag="PKM23_13865"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNY92605.1"
                     /translation="MRFSILVPLFFAFAGAAAAQQSLPECVRIALARNPGLKAAESDA
                     GIAAADVVQAKAARLPALDFSGSYRRQSTVPELNFDSPLFQQFFPAGGVKLGALDNSD
                     LRLTVSQPIFSGFRLSQSRTAAAALAEASRGELARQRNELIFRVESAYAALLRAQKGA
                     EIARSAREQIAAHLRDVEVMVEQGLARRDERLRVEVKASEAELVLLQAENGVALAEAA
                     LENLLATPLPAGEMYAEMGPGRLEPGDLASSLALALANRPELERLARAADAAAAGKKI
                     ARGGRLPNVAGFASYGYGKPGLDMIRNEWMNYWLVGVGAEWNLWNWGRTGAKVEQAEL
                     "
     gene            complement(1028..1639)
                     /locus_tag="PKM23_13870"
     CDS             complement(1028..1639)
                     /locus_tag="PKM23_13870"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNY92606.1"
                     /translation="MRDRIKQATAEVFGERGYDGARMQEIADRAGANKAMIYYYFASK
                     EALFTAIFSENFAALFQLFNSILQVEAVDPKVVIPQLVHLHLDFLSRNPELPRMIIRE
                     IQSGNPVVEKLVQSSFSRFGSQLQTLREEMAVAIREKRLREVDPLQTVWNIVALNIFI
                     FIVRPILSAAFPDEFADAGRMLAEREKAIVDLMLYGLVPRQEA"
     gene            2032..2214
                     /locus_tag="PKM23_13875"
     CDS             2032..2214
                     /locus_tag="PKM23_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92607.1"
                     /translation="MGNQPIYFWQLITSALGLFLVIHAMIARDFLFFNGDERLMRISK
                     FFLGLALSLLPFFDRF"
     gene            complement(2284..>3214)
                     /locus_tag="PKM23_13880"
     CDS             complement(2284..>3214)
                     /locus_tag="PKM23_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNY92608.1"
                     /translation="GGDRAGGYAFAKQQLLRPAADINPVPELAEMEARVVASANRLGI
                     GPMGFGGRLTLGACKIGSLNRLPASFFVSIAYMCWAFRRRGMMLDREGGVQSWLYQEE
                     GEFAAEPAAPAEMNFDPARVRRLQTPLDEAAVRALQVGDIVLLKGTIFTGRDALHKYL
                     HQGGELELLRGGVIYHCGPVVIGTRGQYQVMAAGPTSSIREEPYAAEIIAKYNLKAII
                     GKGGMGERTRQALQEHGAVYLHAIGGAAQIYARCVEEVAGLHLEELGGPEAVWELRVK
                     DFPVVVTIDAHGQSLHREVLADSAARLKAVLQE"
BASE COUNT          591 a         1075 c          925 g          623 t
ORIGIN      
        1 cagttcggcc tgctccacct tcgcgccggt cctgccccag ttccagaggt tccattcggc
       61 gccgactccc accagccagt agttcatcca ttcattgcgg atcatgtcaa gcccgggctt
      121 gccgtaaccg tagctggcaa agccggcgac attgggcagc cgccctccgc gggcgatttt
      181 ttttcccgcc gcggcggcat cggccgcccg cgccagccgc tccagctcgg gccggttcgc
      241 cagcgccagc gccagagaac tcgcgagatc cccgggctcc aggcggccgg gtcccatctc
      301 cgcatacatc tcgccggccg gcagaggcgt ggcgaggagg ttttcaaggg cggcctccgc
      361 cagtgcaacg ccattctcgg cctggagcag gaccagctca gcttcggagg ccttgacctc
      421 gacgcgcagg cgctcatcgc gccgcgccag gccctgctcg accatgacct cgacatcgcg
      481 cagatgggcg gcgatctgct cccgggcgct gcgggcgatc tccgccccct tctgcgcccg
      541 cagcaaagct gcataggccg attcaacccg gaaaatcaac tcgttgcgct ggcgcgcaag
      601 ctcgccccgg ctcgcttcag ccagggctgc cgccgccgtc cgcgactgac tcagacggaa
      661 accggaaaag atcggctggc tcaccgtcag acgcagatcc gaattgtcca gcgcccccag
      721 cttgaccccg ccggccggaa aaaactgctg gaaaagaggg gaatcgaaat tcaattccgg
      781 cactgtgctc tgccgccggt agctcccgct gaaatcgaga gcgggcagcc tcgccgcttt
      841 cgcctggacg acatcggcgg cggcgatccc cgcgtcgctt tcagccgctt tcaggcccgg
      901 gttgcgggcg agggcgatgc gcacacattc cggcaggctc tgctgagccg ccgccgcccc
      961 ggcaaacgca aaaaagaggg ggacaaggat ggagaaacgc atgataactc ctgatcggga
     1021 tgacatttca cgcctcctgg cgcggaacaa gaccgtaaag catgaggtcg acaatggctt
     1081 tctcccgctc cgccagcatc ctgcccgcat cggcgaactc gtccggaaag gccgcggaga
     1141 ggatcggtcg aacgatgaaa atgaaaatat tgagcgccac gatattccag accgtctgca
     1201 gaggatcgac ctcacgaagc cgcttttccc ggattgccac cgccatctcc tctctcaggg
     1261 tctgcagctg gctgccgaag cggctgaaac tgctctgcac cagcttctcg accaccggat
     1321 tgccgctctg gatctcgcgg atgatcatcc ggggcagctc gggattgcgg ctgaggaaat
     1381 cgagatgaag atggaccagc tgcgggatca ccactttcgg atcgaccgcc tcaacctgga
     1441 gaatggaatt gaagagctga aaaagagcgg caaagttttc ggaaaagatg gcggtgaaga
     1501 gcgcctcctt ggaggcaaaa tagtaataga tcatcgcctt gttggcgccg gcgcggtcgg
     1561 cgatctcctg catcctcgct ccgtcatatc cccgctcacc aaagacctcc gcggtcgcct
     1621 gcttgatcct gtcgcgcata ttctcccgat cgctcatctc ttttcctctg cgcctctccg
     1681 ttcccgccgt ggaacgtgcg gaacaaccgg cgatcgcccg gctccgggag ggattatttt
     1741 tccctgcttt tcatattaac caaatggttt aaccaaccgg ttagttctat atactacatc
     1801 ttgtggagtt tgtcaagttt attctgcgca taacggcaat atttgccgac ctgaaaaaaa
     1861 agtcgctact tgtgaagaaa acatttgact ttatgcagca tggcgtctat atttactcaa
     1921 gctgtttctg acgctacatc tccccctcta attgtgctca cttcgccgcc gtccccccca
     1981 cggcagtgga gttctcaggt tccatctcag gcatcgtcag gagaagcagc catgggaaac
     2041 caacccatct acttttggca gctgatcacc agcgccttgg ggctttttct ggtcatccac
     2101 gccatgatcg ccagggattt tctcttcttc aacggtgatg aaaggcttat gcggatctcc
     2161 aaattcttcc tcggcctggc gctctcgctt ctgccctttt tcgaccggtt ctgaagagct
     2221 gccaaaacgc aaaaagcgcc cggaatgtcc ccctccgggc gcttttttta tcccctgacc
     2281 cctctactcc tgcagcaccg ccttgagacg ggccgccgaa tctgccagca cctcgcggtg
     2341 gagggactgg ccatgggcgt cgatggtcac cacgacgggg aaatccttca cgcgcagttc
     2401 ccagaccgcc tccggcccgc cgagctcctc gaggtggagg ccggcgacct cctcgacgca
     2461 gcgggcgtag atctgcgccg ctccgccgat ggcatggaga tagaccgctc cgtgttcctg
     2521 cagggcctgg cgcgtcctct cccccatccc gcccttgccg atgatcgcct tgaggttgta
     2581 tttcgcgata atctcggcgg catagggctc ttcccggatc gagctggtcg gccccgccgc
     2641 catcacctgg tactgacccc gtgttccgat caccaccggc ccgcagtggt agatgacgcc
     2701 gccgcggagg agttccagct cgccgccctg atgcagatat ttgtggagtg cgtcccgccc
     2761 ggtgaagatc gtgcctttga gcagcacgat gtcgccgacc tgcagggcgc gcaccgccgc
     2821 ttcatcaagg ggggtttgca gccggcgcac ccgggccggg tcgaagttca tctccgcggg
     2881 ggcggcgggt tcagcagcga attcgccctc ctcctgatag agccatgatt gcactccccc
     2941 ctcgcggtcc agcatcatgc cccggcgccg gaaggcccag cacatgtagg cgatgctgac
     3001 aaagaaagag gccggcaggc ggttgaggct gccgatcttg caggcgccga gggtcagccg
     3061 gccgccgaaa cccatcgggc cgatgccgag gcggttggcg gaggccacca cgcgcgcctc
     3121 catttcggcc agctccggca ccggattgat atcagcggcc gggcgcagga gctgctgttt
     3181 ggcgaaggca tagcctccag cgcggtcacc tccg
//
LOCUS       DAOPWU010000375         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_31198_length_3178_cov_4.027858, whole genome shotgun sequence.
ACCESSION   DAOPWU010000375 DAOPWU010000000
VERSION     DAOPWU010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31198_length_3178_cov_4.027858"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PKM23_13885"
     CDS             <1..305
                     /locus_tag="PKM23_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92609.1"
                     /translation="LVWLLLIDLVFAGLIATYSPMLAAVFFLRAVVLGALMVVMAVTL
                     AAICFPDLKRAQRRYIYLLVAAVILSALFYRYHPWILIALLLLPAQRLLRMRYYRT"
     gene            377..1072
                     /locus_tag="PKM23_13890"
     CDS             377..1072
                     /locus_tag="PKM23_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY92610.1"
                     /translation="MAYDDCVILSELSLDLERGSLIGLIGPNGAGKTTLMLALSGQFR
                     PRRGAIRFDGMDIYERNLAFKYRIGYVHEAPFFYPHLTAEEFLQFIARVKRVPSAELD
                     LRVNRLLAQVCLNEERSKLTSELSQGMKKKLAIAAALIGAPDLLFLDEALNGVDVESA
                     YQIKEGLREFTARGGTVLLSTHVLEVIEKLCDRYVVMKKGRIIDNVRAADWKETGRSS
                     LEEYIIERLRRLD"
     gene            complement(1404..2105)
                     /locus_tag="PKM23_13895"
     CDS             complement(1404..2105)
                     /locus_tag="PKM23_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92611.1"
                     /translation="MKRTLLLTLFILTLGWTLAAFAGQQDFVLHNQTGLTIDQFYCSP
                     TTTQEWEEDVLGVDVLEDEASAEIRFSRNENSCNWDLMIVDEEGDRIVWSNVNLCEAV
                     RITLYYANGQPTAQIEKAEAEEQDEEEAEDESTEGGAQDFILVNRTGLTIDQFFCSPS
                     KSNNWEEDVLGMDVLEDEASAGITFSREEVSCKWDLMIVDEEGDKIVWSNIDLCKTSR
                     ITLFYDNGQPTAAAE"
     gene            complement(2871..>3178)
                     /locus_tag="PKM23_13900"
     CDS             complement(2871..>3178)
                     /locus_tag="PKM23_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92612.1"
                     /translation="LLALTAQLLVMAVLWIFPFFERRIDLVRETRTYEIILPLRSEKI
                     EAISSDLAASGVTVISRKVVKGKDHLSVSIEAYGAEESFAKLTGKLIQDPDVHEFKC"
BASE COUNT          650 a          884 c          886 g          758 t
ORIGIN      
        1 cactggtctg gctgctcctc atcgatctgg tttttgccgg gctgatcgca acgtactcgc
       61 caatgttggc tgcggtcttt ttcctcagag cggtggtgct cggtgcgctc atggtggtga
      121 tggcggtaac gttggcggct atttgcttcc cggacctgaa acgggcgcag cggcggtata
      181 tctacctgtt ggtggctgca gtcattctct ctgctctgtt ttatcgttac catccctgga
      241 tcctcattgc tttgctcctt cttccggcgc agcgcctgct gcggatgcgc tactatcgaa
      301 cgtgaacctt ctgaacgaaa ggatgtgact ggtggagtcc tccgggcaaa acaggctgca
      361 tgttgacgcc atccaagtgg cctatgatga ttgtgtgatt ctgtcggagc tgtcgctgga
      421 tctggagcgc ggttcgctga tcggcctgat cggtccaaac ggcgccggca aaaccacgct
      481 gatgctggcg ctttcgggtc aattccggcc gcgccgcggc gccattcgct ttgacggcat
      541 ggatatttat gagcggaacc tggcctttaa atatcgcatc gggtatgtgc atgaggcgcc
      601 ctttttctac ccgcatctta ccgccgagga attcctccaa tttatcgccc gggtgaaacg
      661 ggtgccgtcc gcggagttgg acctccgagt caaccggctg ctggcacagg tttgcctgaa
      721 tgaggagcgc agcaagctga ccagcgaatt gtcgcagggg atgaagaaga aactggcgat
      781 cgccgccgct ctcatcggcg cgcccgacct gctctttctc gatgaggcac tcaacggcgt
      841 ggatgtggag agcgcctatc agatcaagga gggcttgcgc gagtttaccg ctcgcggcgg
      901 caccgtcctg ttatcgaccc acgttctgga ggtgatcgag aagctctgcg accggtatgt
      961 ggtgatgaaa aagggaagga tcatcgacaa tgtcagggct gccgactgga aggaaacggg
     1021 ccggtccagc ctggaggagt atattatcga gcggttgcgc cggctggact agcaatcctt
     1081 gccctgcaag cagtcatgcc ggaatgtgta gcaggcgggt atctgatgca atctggcaat
     1141 atagcgctaa aaaattcata cttatgactt gatgcacctt cgccagagag cccctgttat
     1201 tttttttgca gagacataat ggccggcatg cacgggcggg caggtgggga cgagaaaacg
     1261 cgacctggca atgtcgtgtc cgtacagcag cacccgggct ggcaaaggcc gtgcacaaaa
     1321 ccctggcagg gccatcaatt cctgttgcca tctgtgcacg gccacctggc ttgccgtgct
     1381 gccagcccgc agtggaaacc ggtttattcc gccgcagccg tgggttggcc gttgtcgtaa
     1441 aaaagcgtga tccgggaggt cttgcagaga tcaatattgg accagacaat cttgtcgcct
     1501 tcctcatcaa cgatcatcag atcccatttg caggaaacct cttcgcgtga aaatgttatg
     1561 ccggcactcg cttcgtcctc cagtacatcc atgcccagta catcctcctc ccagttattg
     1621 cttttactgg gtgagcaaaa gaactggtcg atagtcaggc cggttctgtt gacgaggatg
     1681 aaatcctgcg cgccgccttc agtggactca tcctccgctt cctcctcatc ctgctcttca
     1741 gcttccgcct tttcgatttg ggctgtaggc tgtccgttcg cataatagag ggtaattctc
     1801 acagcttcac aaagattcac attagaccag acaatcctgt cgccttcttc atcgacaatc
     1861 atgaggtccc agttacagct gttttcgttg cgggaaaatc tgatttcagc gctggcttca
     1921 tcttccagaa catcaacacc cagtacatcc tcttcccact cttgcgtggt cgtgggcgag
     1981 cagtagaact ggtcgatggt caggccggtt tgattgtgca ggacgaagtc ctgttgaccg
     2041 gcaaaagcgg ccaaagtcca gccaagggtc aggatgaaca gggtgagcaa caaggtgcgt
     2101 ttcatgagtt ctcctttcgg gatcggggaa attgttcact tttaatatct gacaggatca
     2161 tcctggcggg atgaaggatt ggagtgaatt ggaaacgatt gctattggag tcgaagatgc
     2221 gatagaatca cccgtcgtca cgtgccggtt acacaaccgg ttcctcctgt gcagaaccag
     2281 gctgtcatcc ggcgcccaca tcactgcctg ccggtttgaa aattcctgct tggcacctga
     2341 aaatcaggca atttatttaa agattaacct ggatctcaac aggattattc cctgcatggg
     2401 cgaggcatag agttgtaaaa cgaagcagat gggaacgaga ggaaacgaag cagatggaaa
     2461 ggagaggaat cttgcgccca cccggcggcc ctcaccatca ggcgccgacc aggcagaccg
     2521 caactctggc gcccatccgg cggccaacac aatctggcgc caacccggca ggccccccaa
     2581 tctggcgcca acccgcttgc ttctattggg gcttcggttc ttttgccgcg cggtcacgca
     2641 cctcccgtgg atagtccgaa aaggccaatt tgtcatccag ttctcccctt tcagcccgtt
     2701 gccgtaattc ctcagcatcc gcctcggttt cggggatcca tattctgacc tctactccat
     2761 cgaccgtgtg ttcgatcaca gttctcatct gcacgcttcc ctttcctgtt ttttgggcga
     2821 ggttatcatg ctggcctcca tcgtctcatg gtaggatcca acgggaccct tcagcatttg
     2881 aattcgtgta catcggggtc ctggatcagc ttgccggtga gtttggcaaa actctcctcc
     2941 gcgccgtaag cctcgatgct gaccgagaga tggtccttgc ccttgaccac cttgcggctg
     3001 atcaccgtca ccccgcttgc ggccaggtcg ctggaaatcg cctcgatttt ttcggagcgc
     3061 aggggcagga tgatttcgta ggtgcgggtt tcgcgcacca ggtcgatgcg gcgttcgaaa
     3121 aaggggaaaa tccacagcac ggccatgacc agcagctggg cggtcaaagc gaggagca
//
LOCUS       DAOPWU010000376         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_31212_length_3177_cov_4.607944, whole genome shotgun sequence.
ACCESSION   DAOPWU010000376 DAOPWU010000000
VERSION     DAOPWU010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31212_length_3177_cov_4.607944"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PKM23_13905"
     CDS             <1..1299
                     /locus_tag="PKM23_13905"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNY92613.1"
                     /translation="ASIPVILRGDDMIGTAQTGTGKTAAFVLPILHRLLSGPRHQSRA
                     LILTPTRELAEQIHQAVNSLSRGTGLRSATIYGGVPPAAQIKALRSGVEILVACPGRL
                     LDHLDQRHADFSHIEFLVLDEADRMLDMGFMPSVRKILQRVPERRQTLLFSATFPEEI
                     ETLARQSMRRPERIALGLSAPVKTVSHALYPVEQHLKLPLLLALLQQTEAGSVLVFTR
                     TRHRAQRVARQIGQHGYRVTSLHSDRTQSQRQAALQGFRRGDFQIMVATDIAARGLDV
                     ESISHVINFDMPDTADAYIHRIGRTGRAERSGDAYTMTTSEDNEMVRTLEKIMGKPLP
                     RQTLQNFDYQQAAPPKRPDEQRPQTRSSMPRRSTASQRPAPQRSGVRSNHPRPAGQAQ
                     RSRAPRAQARSISQQQAEQEASTSVIARWGRTLRSRRRTL"
     gene            1373..1531
                     /locus_tag="PKM23_13910"
     CDS             1373..1531
                     /locus_tag="PKM23_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92614.1"
                     /translation="MKNRWKIMGIAMILIMVIAISCFMLTGGDCNRSCDLFKEVEEER
                     DDFQVVVR"
     gene            1506..>3177
                     /locus_tag="PKM23_13915"
     CDS             1506..>3177
                     /locus_tag="PKM23_13915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY92615.1"
                     /translation="MISKLWCGKTIFSGRCSPSVLLLCASFCILTIPAAALSAKVSLP
                     KAKEKWICREGELFTLYSAAAAKQAGEISLGLAQYHAVLAGQSGGTAIPSPVPTFIYV
                     FKDERGLAPYQCFPLQQPSGSGGYFLQREDAQYIAVSAREEVTRTIYHEYAHAIFATA
                     WPGAPLWLQEGLAEYFSTFRSDGQHVYIGEPLKEHARTLRASVCIPFAELMELDADAS
                     LYRDSDKNPIFYAQAWIVTHFLFHGLKEGGAAAIDRYITLLGSGESNARSFAEAFHIS
                     LADLDAAVKNYAEQPALGFTRLAARQVAIGDDPPFRAVPYEEILYRLGDLLAHHGQSQ
                     LVAAEAHFRQAAKVKSTYALAPAGLGYAALAREDETTALGHFQQALALDSTSAWIYYQ
                     YGRCLLAAGRKQEAPQKQESMARARDALLRTLALDSRFIEAQAQLGEAYMHLGMQPQG
                     QTALEKALAGMPSRMDVARNLLQYYVLAENSARQTELLTLIGQRGGAKAAEAARESIL
                     RTQIDRVSRLINDKEYARALALAEKLRKQTDDPALKSLIDEARQAAAQG"
BASE COUNT          599 a         1094 c          922 g          562 t
ORIGIN      
        1 gcctccatcc ctgtgattct gaggggggac gatatgatcg gaaccgccca gaccggcacc
       61 ggtaaaaccg cagcctttgt gctgcccatt cttcaccgtc tgctttcagg gccacgccat
      121 cagagccgcg ccctgatcct gaccccgacg cgcgagttgg ccgaacagat ccatcaggct
      181 gtgaacagcc tgagcagggg taccgggctc cgcagcgcca ccatttacgg cggcgtgccg
      241 ccggcagcgc agatcaaggc cctgcgcagc ggcgttgaga tcctcgtcgc ctgtcccggc
      301 cgtctgctcg accatctcga ccagcgtcat gccgatttct cccacatcga atttctcgtc
      361 ctcgatgagg cggatcggat gctggacatg ggctttatgc cctcggtgcg caagatcctg
      421 cagcgggtgc cggagcggcg ccagactctg cttttctccg ccacctttcc cgaggagatc
      481 gaaaccctgg ccaggcagtc gatgcgccgg ccggaacgta tcgccctcgg gctgagcgcg
      541 ccggtgaaaa ccgtcagcca cgccctttat ccggtcgagc agcacctcaa gctgcccctg
      601 ctgctggcgc tgcttcaaca gaccgaggcc ggctcggttc tggtcttcac ccgcacccgc
      661 catcgggccc aacgcgtcgc ccgccagatc gggcagcacg gctaccgggt gaccagcctg
      721 cacagcgacc gcacccagag ccagcgccag gccgccctgc agggcttccg ccgcggcgat
      781 ttccagatca tggtcgccac cgacatcgcc gcccgcggcc tggatgtgga gagcatctcc
      841 catgtcatca actttgatat gcccgacacc gccgatgcct acattcaccg catcggccgc
      901 accggccgtg cagagcgcag cggggatgcc tacaccatga ccacgtccga ggacaacgag
      961 atggtcagaa ccctggagaa gatcatgggc aaaccgctgc cgcgccagac cctgcagaat
     1021 ttcgattacc agcaggcggc accgccaaaa cgccctgacg aacagcggcc gcaaacgcgc
     1081 agcagcatgc cccggcgttc aacggcgtcc caacggcctg caccacagcg gtcaggggtg
     1141 cgcagcaacc acccgcggcc ggctggacag gcgcagcgtt ccagggcacc gagagcgcag
     1201 gcgaggagta tctctcagca gcaggccgag caggaggcga gcacttccgt catcgcccgt
     1261 tggggccgta cactgcgttc ccgccgccgc accctgtaaa gaaaatgttt tgaattgggg
     1321 atgattattg cgatatttta ttgataagta tcggaaataa cgaatcatcc ccatgaaaaa
     1381 ccgctggaaa atcatgggaa tcgcgatgat cctgatcatg gtcatcgcga tttcctgttt
     1441 tatgctgacg ggcggggatt gcaatcgtag ctgcgatctt ttcaaggaag tagaagagga
     1501 gcgcgatgat ttccaagttg tggtgcggta aaaccatctt ctccggccgg tgctcaccct
     1561 ctgttctgct cctctgcgct tcattttgca tcctgaccat ccctgctgca gccctttccg
     1621 ccaaggtgtc cctgcccaaa gccaaagaga aatggatctg ccgggagggc gaacttttca
     1681 ccctctacag cgcggccgcg gccaaacagg ccggcgaaat cagtctcgga ctggcccagt
     1741 atcatgccgt gctcgccggc cagagcggcg gcaccgccat cccctcgccg gtgccaacct
     1801 ttatctatgt cttcaaggac gagcgcggcc tcgcccccta tcaatgtttc ccccttcagc
     1861 agccctccgg cagcggcggt tattttctcc agcgcgagga tgcccaatac atcgccgtca
     1921 gcgcccggga agaggtgacc cgcaccatct atcatgaata cgcccatgcc atcttcgcca
     1981 ccgcctggcc gggagcgccc ctctggctgc aggaggggct ggcggagtat ttcagcactt
     2041 tccgctccga cggacagcat gtctacatcg gcgagcctct caaggagcat gcccgcaccc
     2101 tgcgcgccag tgtctgcatc ccctttgccg agctcatgga gctggatgcc gatgcctcgc
     2161 tctaccggga ttccgacaag aaccccattt tttacgccca ggcctggatt gtgacccact
     2221 tcctcttcca tggcctgaag gaggggggcg cggcggcgat cgaccgctac atcaccctgc
     2281 ttggcagcgg ggagtccaat gcccgcagct tcgccgaagc ctttcatatt tctctggccg
     2341 atctcgatgc ggcggtcaag aactacgccg agcagcctgc gctggggttt acccgtctcg
     2401 ccgcccggca ggtggccatt ggcgatgatc ctcctttccg ggccgtgccc tatgaggaga
     2461 tcctctaccg actgggggat ctgctggcgc accatggcca gagccaactc gtagcggcgg
     2521 aggcccactt ccgccaggct gccaaagtga aatcaacata tgcgctcgcc cccgccggat
     2581 tgggttatgc cgccctggcc agggaggatg agaccaccgc ccttggccat tttcagcagg
     2641 ccctggcact ggactcgacc tcggcctgga tctattatca atacggccgc tgcctcctcg
     2701 ccgccggccg caagcaggag gccccgcaga aacaggagag catggcgcga gcgcgcgatg
     2761 ccctgctgcg tacgctcgcc cttgacagcc gcttcatcga ggctcaggcg cagctcggcg
     2821 aagcctacat gcatctgggc atgcagccgc aggggcagac cgcccttgaa aaagccctcg
     2881 ccggcatgcc ttcacgcatg gatgtcgccc gcaatctgct ccagtactat gtcctggcgg
     2941 agaattccgc ccggcagacc gaactgctga ccctcatcgg ccagcgcggc ggggccaaag
     3001 cggctgaggc cgcccgcgag tcgattctgc gcacgcagat cgaccgggtc tcccggctga
     3061 tcaacgacaa ggaatacgcc cgggccctcg ccctggcgga gaagctgcgc aagcagaccg
     3121 acgaccctgc gctcaaaagc ctgatcgacg aggcccggca agcggcggca caggggc
//
LOCUS       DAOPWU010000377         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_31213_length_3177_cov_3.713004, whole genome shotgun sequence.
ACCESSION   DAOPWU010000377 DAOPWU010000000
VERSION     DAOPWU010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31213_length_3177_cov_3.713004"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>882)
                     /locus_tag="PKM23_13920"
     CDS             complement(<1..>882)
                     /locus_tag="PKM23_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92616.1"
                     /translation="DAVRSLLTTTARDLGPQGWDIYYGAGRLDLARALAPGLAAEVRI
                     DSPWLDQGFQQSPIEIRGSAWTPSLQSYTLEWGMGDNPSAWSPITPPRSSRVIDGLLG
                     LWEDFPAADTSYTIRLRIQNSNGSVEQSQVRIFIDRTAPLISNIETISMYDGGSPSCL
                     LQFVTDDLCEGEILFRPAGSEAPWQEAPMSYRSYQPRLHLTRDLGAQRLDLQIRVDNP
                     AGLRTTSDSLLRVDLSAPPLNTMRYTRLEESLPHGHLLASSADFNGDSRPEIVLATAD
                     EQGRLFTALYTREASGFR"
     assembly_gap    883..892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<893..2133)
                     /locus_tag="PKM23_13925"
     CDS             complement(<893..2133)
                     /locus_tag="PKM23_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNY92617.1"
                     /translation="MVLLAFLTAAGVAAAPPAARAQSQTLLVKMSLPLPAAAFDDAGA
                     LRKEGAAALSALAARPEITAAAPFLPRILSFGRDGGDAALPERAELAKWLILTLADTA
                     DGDAIAGELQHHAEVSWSGRPRCFHLDWIPDDPLFSRQYGLQRISAPTAWESERGSRG
                     VIVGVIDTGIDYDHPDLRENLWINHGEDLNRDGRADSLDLNGLDDDGNGWIDDLQGYD
                     FTDAPNYPDGGDYRGRDADPMDEMGHGTAVAGIIAAVAGNDLGIAGAAPGCRVMNLRA
                     FTAGGNGEEDDVAAAILYAIENGAAVINMSWGDVFVSRLLDDVLHYAAALNTVLVASA
                     GNSATDQIHYPSAFANTISVGATDASDQLAGFSNYGPSIDLVAPGVNILTAARGAQYD
                     SSLSGTSFSAPFVSAAAALLLS"
     gene            complement(2169..3056)
                     /locus_tag="PKM23_13930"
     CDS             complement(2169..3056)
                     /locus_tag="PKM23_13930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY92618.1"
                     /translation="MVISVVIVTYNNHAEIGACLDALHLASASFTTHLFLIDNASTDG
                     TGELLEEYRSRCASHFHRFLVIRNGQNRGFTAAVNQGLLHCRGDFFLLLNPDVVIPEG
                     ALAVLLRQFERDPAIGVVAPQLRFPDGRIQPSCRRFPRRRDVLLEVSGLVHLARRLGF
                     KDWKMTDFDHRHSRFVDQPQGAFLLARQELLDRLGGLDERYFMFFSDVEWCERVIASG
                     WKIWFCSDTFVRHHKGASVYRERPRMLVTSTRSFIDYLSAHDRSRCDRFATAVVALIL
                     KLTLQVRLLAEKARETLTP"
BASE COUNT          565 a          902 c         1184 g          516 t
ORIGIN      
        1 cggaatcccg aggcctcccg ggtatagagg gcggtgaaga gcctgccctg ttcgtcggca
       61 gtggcgagga cgatctcggg ccgggagtcg ccgttgaaat cggcgctgct ggcgagcaga
      121 tggccgtggg ggaggctctc ttcaaggcgg gtataacgca tggtgttgag tggtggggcg
      181 ctgagatcga cgcgaagcag gctgtcggag gtggtgcgca gcccggcggg attgtcgacg
      241 cggatctgca ggtcaagcct ctgcgccccg agatcgcggg tgagatggag gcgcggctgg
      301 tagctgcggt aactcatcgg cgcctcctgc cagggggctt cactgcccgc gggacggaag
      361 aggatttctc cctcacagag gtcatcggtc acgaactgaa gcaggcagga agggctgccg
      421 ccgtcgtaca tggagatggt ctcgatattg gagatcagcg gggcggtgcg gtcgatgaag
      481 atgcggacct gggactgctc gacggagccg ttgctgttct ggatgcgcag gcggatggtg
      541 tagctggtgt cggccgccgg aaaatcctcc cagaggccga gcaggccatc gatgacccgg
      601 ctggaacggg gcggggtgat cggggaccag gctgaagggt tgtcccccat cccccactcc
      661 agggtgtagc tctgcagtga gggggtccag gcggagccgc ggatttcgat cgggctctgc
      721 tggaagccct ggtcgagcca gggggaatcg atgcggacct cggcggcgag gccgggggcg
      781 agagcgcggg cgagatcgag ccggccggcc ccgtagtaaa tgtcccagcc ctgggggccg
      841 aggtcgcggg cggtggtggt caggagactg cgcaccgcat cgnnnnnnnn nngagagcag
      901 cagggcagct gcggcggata cgaagggagc cgaaaaggag gtgccgctga gggaagagtc
      961 gtactgcgct ccgcgtgcgg cagtgaggat atttacgccg ggggcgacga ggtcgatcga
     1021 ggggccatag ttggaaaagc cggcgagttg gtccgaggcg tcggtggcac cgaccgagat
     1081 ggtgttggcg aaggccgagg ggtagtgaat ctggtcggtg gcgctgttgc cggcggaggc
     1141 gaccaggacg gtattcaggg ccgcggcgta gtggaggacg tcatcgagga ggcgggagac
     1201 gaagacatcg ccccagctca tattgatcac cgccgcaccg ttttcgatgg cgtagaggat
     1261 ggcggcagcg acgtcgtcct cctcgccgtt gccgccggcg gtgaaggcgc gcaggttcat
     1321 cacccggcag ccgggggcgg cgccggcgat gccgaggtca ttgccggcga cggcggcgat
     1381 gatgccggct acggccgtgc cgtgccccat ctcatccatc gggtcggcat cgcggccgcg
     1441 atagtcgccg ccgtccggat agttgggggc gtcggtgaaa tcgtagccct gcaggtcatc
     1501 gatccagccg ttgccgtcgt cgtccaggcc gttgaggtca agggaatcgg cccggccatc
     1561 ccggttgaga tcctcgccgt ggttgatcca gagattctcc ctgagatcgg ggtgatcata
     1621 gtcgatgccg gtatcgataa caccgacgat gaccccgcgg ctgccgcgct cgctctccca
     1681 ggccgtcggg gcggagatcc tctgcaggcc gtactgccgg ctgaaaaggg gatcgtcggg
     1741 gatccagtcg agatggaagc agcgcggccg gccgctccag ctcacctccg catggtgctg
     1801 cagttctccg gcgatggcat cgccatcggc cgtgtccgcc agggtgagga taagccattt
     1861 tgccagttcg gcgcgctccg ggagggcggc atccccgcca tcgcggccaa aggatagaat
     1921 gcgcggcagg aacggtgccg cggcagtgat ctccggccgt gcggcgaggg cgctcaaggc
     1981 ggcggcgccc tccttgcgca gggcgcctgc gtcgtcgaat gcagcggccg gcaggggcaa
     2041 gctcatcttg accagaaggg tttgtgactg cgcccgggcc gccgggggtg cggctgcaac
     2101 tccggcggcg gtcaggaacg ccaggaggac caatccagcc ctccttccta tacgtggaag
     2161 cagcagtttc atggagtgag tgtttccctg gccttttcgg cgagcagacg cacttgcagc
     2221 gtcagtttca ggatcagcgc gacgacggcg gtggcgaagc ggtcgcagcg ggagcggtcg
     2281 tgggcgctca ggtagtcaat aaaagagcgc gttgaagtga ccagcatacg cggccgctcg
     2341 cgataaacgc tggccccctt gtgatggcgg acaaaggtat cgctgcagaa ccagatcttc
     2401 cagcccgagg cgatgacccg ctcgcaccat tcgacatcgc tgaagaacat aaagtagcgc
     2461 tcatccaggc cgccgaggcg gtcgaggagc tcctgccggg ccagcaaaaa ggccccctgg
     2521 ggctggtcga cgaagcggct gtggcgatgg tcaaaatcgg tcatcttcca gtccttgaag
     2581 cccagccgtc gggcgagatg gaccaatcca ctcacctcga ggagcacatc acgccggcgg
     2641 ggaaaacgcc ggcaggaggg ctggatgcgg ccgtcgggaa aacggagctg gggcgcaacc
     2701 accccgatgg cgggatcgcg ctcgaactgg cgcagcagca cagccagtgc gccctccggg
     2761 atgaccacat cgggattgag aaggaggaag aaatcgccgc ggcagtgtag aagcccctgg
     2821 ttgacggccg ccgtgaagcc gcggttttgc ccgttccgga tgacgagaaa ccggtgaaag
     2881 tgtgaggcgc agcggctgcg gtactcctcc agcagctcac cggtgccgtc ggtgctggca
     2941 ttgtcgatga ggaaaaggtg cgtggtgaaa gatgccgagg caaggtggag cgcgtcgagg
     3001 caggcgccga tttctgcgtg gttattatag gttacgatca caacagagat gaccacggct
     3061 gtccatttcc accggtgaga gcgaacaagg gctgcagcgc ttccgcgggc ggaggcgatg
     3121 ccgtcccggg ggcgctgcag tgcggaggct acttgctttc atcctccagg cgtttgc
//
LOCUS       DAOPWU010000378         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_31240_length_3175_cov_2.792949, whole genome shotgun sequence.
ACCESSION   DAOPWU010000378 DAOPWU010000000
VERSION     DAOPWU010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31240_length_3175_cov_2.792949"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PKM23_13935"
     CDS             complement(<1..431)
                     /locus_tag="PKM23_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92619.1"
                     /translation="MDQNLILGLFYRHRRVLGTWSRAVLRMLVFSALFLMPTMAQAVV
                     NLKITKLADEGTVASGATITYRIQYTVASTTENAINAVITDVLPVELASSASDVTLEG
                     DDFVTSAIYTAATRTARFVFQSPLAASTTGTNTTRAIVTFK"
     gene            complement(928..>3175)
                     /locus_tag="PKM23_13940"
     CDS             complement(928..>3175)
                     /locus_tag="PKM23_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY92620.1"
                     /translation="RSAYANSIGAAWFHSIHSNATGSGGASDVNTALLLWPQLADYSE
                     RLPVGHKRMSDIMIDQLSRGMRIKSIGSRGDCYFYNEGICPRNSVTKYTLMPAELSEA
                     GYHTNMAQNMRQMSAAFNKLEAYTFYWSILKYHGLPLPVNPVLTGIVYDLERNVPINA
                     ATVTAGEKSYTTDSFETTFYKYTTDPEALRNGVFLLDGLPSGTLKVMVSAPDYYSDTL
                     QVALSDTFFTFLDPKLIWKRPPAVKSSSPAQGYERQPAWGNISITFNRPLNRASFESA
                     FKIEPAVSGQFVWGNNDQQMIFKPDTLDFETDYQVTIAATGVDRYGHPLDGNGDRVGG
                     DEFVLNFRTGPRDMAAPRITAVYPPTNTAAVDARPILNITFDEVIDAGSIQPALFTLQ
                     KNGTTTPIPHTVVHNVVRSRSILSFAPSEDLEPGQLYSLLIAPGLRDTLGNEESGIKT
                     VRLKTGAAFWRTRAIDAFENGVENWWQPSASGTTTGTIADSTRSYGSSLVLNPLTSST
                     LSFAIDYGWNTGSPAWLLRDYLFGGPAKAVTFDKSSYLQVYLFGDNSGTLFRFAVDDR
                     VPVAAAENHEVSPWYKVNWYGWRLISWEMNNEAPGSWLGDGRLDGTLAFDSIQLGYEP
                     GSQTSGTLYFDDLRVAEYSAVGVVQAGADVPARFALEQNYPNPFNPSTTILYDLPEGM
                     HEVSVRIYDLLGRPVRALVETRQSAGHYSVQWDGRDDAGRLAASGVYLFRIATPLFNA
                     VKRMVYIK"
BASE COUNT          703 a          796 c         1014 g          662 t
ORIGIN      
        1 tttttgaaag tgacgattgc gcgcgtggtg ttcgttccgg tcgtgctggc cgccaggggg
       61 gattgaaaga caaaccgggc ggtgcgcgtc gcagcggtgt atatggctga ggtgacaaaa
      121 tcatcgcctt cgagggtcac atcgctggca gaagaggcca attcgaccgg tagaacgtcg
      181 gtgatgaccg cgttgatggc attttcggtc gtgctggcga ctgtgtactg gatccggtag
      241 gtgatggtgg ctccagaggc gactgtgcct tcatcggcca gcttggtgat cttgagattg
      301 accacggcct gtgccatagt cggcatgagg aaaagcgctg aaaaaaccag catcctcaaa
      361 acagcgcggg accaggtgcc aagaacacgc cggtgtcggt aaaagaggcc gagtatcagg
      421 ttttggtcca taatccagaa ctccttattt ttctgagctg caacaacgaa tacccaagca
      481 gaaggcatgg catcaacaca cgagaaagag acagcacgac acggcatgat gctctttacg
      541 cggctggatt gccctgcaga gacattaaac cgatgagtat gcgtccgcct caaggcagta
      601 caatcaaacc actgccatgg ttaaaaggcg caataccggt tcaggtactt tgattggatt
      661 ggataaaaaa ggcaaaactc cccaggaacc acaacccgac ccatcgtaca aaaacagatc
      721 actataccaa tatgatgcgg ctgcagcaaa agatgcagag gccaagaggc agttagaata
      781 tacatttttg gcgaattata gtcaagtaaa attatttttt tccttttttt tgtcactttt
      841 ttaactgtat taaatataaa acggcggcaa gcgctgtaaa atgcctgccg ccgtgcaaga
      901 aatgaacagc ttgataaaat cggagtgcta cttgatatag accatccgtt ttacagcatt
      961 gaagagcggg gttgcgatgc ggaagaggta gacaccgctt gcagccagcc ggcctgcatc
     1021 atcgcgtccg tcccactgga ctgaatagtg cccggcggac tggcgggttt cgaccagggc
     1081 gcgcaccggc cggccgagca gatcataaat gcgcaccgaa acctcgtgca tgccctccgg
     1141 aagatcgtag aggatggtcg tcgaagggtt gaagggattg ggatagttct gctccagggc
     1201 gaacctggcc ggcacatccg cccccgcctg cacaactcca acggctgaat attcagcgac
     1261 gcgcagatcg tcgaagtaga gggttcccga ggtctggctg ccgggctcgt agccgagctg
     1321 gatgctgtcg aaagcgaggg tgccgtcaag ccggccgtcg ccgagccagc tgccgggggc
     1381 ttcgttgttc atctcccagc tgatcaggcg ccagccatac cagttgacct tgtaccaggg
     1441 gctgacctca tggttctcgg ccgctgcaac gggaacgcgg tcgtcgacgg caaagcggaa
     1501 aagggtgccg ctgttatcgc cgaagaggta aacctgcagg tagctgctct tgtcaaaggt
     1561 gaccgccttg gcaggtccgc cgaaaagata gtcgcgcagc agccaggcgg gtgagccagt
     1621 gttccagcca taatcaatgg cgaaagagag ggtgcttgat gtcagtggat tgaggaccag
     1681 tgaagaaccg taggaacggg tgctgtccgc aatggtcccc gtggtcgtgc cgctcgccga
     1741 gggctgccac cagttctcca cgccgttttc gaaggcatcg atggcgcggg tgcgccagaa
     1801 ggcggcacca gttttgaggc ggacggtctt gatgccgctc tcctcgttgc caagggtgtc
     1861 gcgcagcccg ggggcgatga gcagggagta gagctgaccg ggttcaaggt cctcggaggg
     1921 cgcaaaggag aggatgctgc gtgagcggac gacattgtgt acgacggtgt gcgggatcgg
     1981 cgtggtcgtg ccattcttct gcagggtgaa gagagcgggc tggatggagc cggcgtcgat
     2041 cacctcatca aaggtgatgt tgaggattgg ccgggcgtcc actgctgctg tattggtcgg
     2101 gggatagacc gcggtgatac gcggcgcggc catatcgcgg gggccggtgc gaaaattgag
     2161 cacgaactca tcccccccca ccctgtcgcc attgccgtcc aggggatggc cgtagcgatc
     2221 gacgccagtg gcggcgattg tgacctggta atcggtctcg aaatcgaggg tgtcgggctt
     2281 gaagatcatc tgttgatcgt tattgcccca gacgaactgg ccgctcacag ccggttcgat
     2341 tttgaatgcc gattcgaagc tggcccggtt cagcggccgg ttgaaggtga tgctgatgtt
     2401 tccccaggcg ggctggcgtt catagccctg cgcgggtgaa gagcttttca ccgccggcgg
     2461 ccttttccag atcagtttgg gatcgaggaa agtgaaaaag gtatcggata gtgccacctg
     2521 cagggtatcc gaataatagt cgggagcgct gaccatgacc ttgagggtgc cggagggcag
     2581 gccgtcgaga aggaagaccc cattgcgcag ggcctcggga tccgtggtgt atttgtagaa
     2641 ggtggtctca aagctgtcgg tggtgtagct cttctcccct gccgtcaccg tagcagcgtt
     2701 gatgggcaca ttgcgctcca gatcgtagac gatgccggtg aggacgggat tgaccggcaa
     2761 gggaaggccg tggtatttga ggatcgacca gtagaaagta taggcctcga gcttgttaaa
     2821 ggcagcgctc atctggcgca tgttctgggc catattggta tggtagccgg cctcgctgag
     2881 ttcggcgggc atgagcgtat atttcgttac tgaattgcga gggcagatac cctcattgta
     2941 aaagtaacag tcccctcgtg agccgatgct tttaatgcgc atgccgcggc tgagctgatc
     3001 gatcatgata tctgacatgc gtttatggcc gacgggaagc cgctcactgt aatcagcgag
     3061 ctgtggccag aggagcagag ctgtgttgac atccgaggct ccgccggagc cggtggcatt
     3121 gctgtgaatg gagtgaaacc aggccgcgcc gatggaattg gcataggctg aacgg
//
LOCUS       DAOPWU010000379         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_31631_length_3149_cov_3.324822, whole genome shotgun sequence.
ACCESSION   DAOPWU010000379 DAOPWU010000000
VERSION     DAOPWU010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31631_length_3149_cov_3.324822"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /gene="rplT"
                     /locus_tag="PKM23_13945"
     CDS             complement(<1..306)
                     /gene="rplT"
                     /locus_tag="PKM23_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNY92621.1"
                     /translation="MPRSHSPVVTRHRRNKYLKAAKGFYGGKNSLYRSARETVEKGMT
                     YNYRDRRARRREFRSLWIVRINAAAHMFGMNYSSLINGLKQKDIDINRKMLAELAVHD
                     "
     gene            complement(341..535)
                     /gene="rpmI"
                     /locus_tag="PKM23_13950"
     CDS             complement(341..535)
                     /gene="rpmI"
                     /locus_tag="PKM23_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNY92622.1"
                     /translation="MPKMKSNRAAKKRFSLTGTGKVRRNKNYARHILTKKSAKRKRSL
                     RQNAIATPADEPRIKRMLSA"
     gene            complement(569..1096)
                     /gene="infC"
                     /locus_tag="PKM23_13955"
     CDS             complement(569..1096)
                     /gene="infC"
                     /locus_tag="PKM23_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNY92623.1"
                     /translation="MRGGQAIKKKLTRVNELIDAPSVRLVDENGEQVGVVSRSDALRR
                     AESAGLDLVEVSPNSAPPVCKIMDFGKFKYELSKKEKETRKKQHVISTKEIRMRPKIE
                     EHDFEFKIKHARKFLLEGDRVRASVQFKGREMIHQEFGRKILERFIEGLQDIAKTEKD
                     PVMEGGQLVVFFVKK"
     gene            complement(1101..3008)
                     /gene="thrS"
                     /locus_tag="PKM23_13960"
     CDS             complement(1101..3008)
                     /gene="thrS"
                     /locus_tag="PKM23_13960"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNY92624.1"
                     /translation="MITLTFPDGRSREYPEGTKASELLSELGGRIAREAIAAKLDAEV
                     LDLSRPLTRSGRILFLTADSPEGMDVFWHSAAHIMAHAVKQLYPEAQFAFGPPVEEGF
                     YYDIDVKESFTPEHLQRIEAKMAEIVAADHPFIREEMDKEEALRLFRERKEGYKLQQI
                     ERLGEPPSIYRDGDFIDLCRGPHLPSTGYVRYFKLMSVAGAYWLGDEKNPMLQRIYGV
                     AYPKKSQLDDYLLRIEEAKKRDHRRLGRELDLYSVNDEIGPGLILWHPKGGFIRYKVE
                     EFWRQEHLKNGYEFVYTPHIARLDLWNTSGHLGFFKENMYAPTEVDEQLYQIKPMNCP
                     FHLQIFRSQTRSYRDLPIRWAELGTVYRYERSGVLHGLMRVRGFTQDDAHHFCRPDQL
                     DAEIDMLLDFTLFILRSFGFSEFEIYLSTRPEHYVGTPENWERATDALRAGLEKAALN
                     YQIDPGEGVFYGPKIDIKIRDSIGRTWQCSTIQVDFNEPERFDLTYVGQDGKEHRPIM
                     IHRALLGSLERFFGVMIEHYAGAFPLWLAPTQVRVLPITENQHDYARKIMDQLTAAGI
                     RAELDDRSEKVGYKIREAETQKIPVMLIVGQKEAEQNQVSVRKHREGDCGLAEIPAVI
                     SEIQEKVLAKA"
BASE COUNT          617 a          846 c          928 g          758 t
ORIGIN      
        1 gtcatgcaca gccaattcag cgagcatctt gcggttgata tcgatatctt tctgtttaag
       61 cccgttgatc agggaggagt agttcatccc gaacatgtgg gcggcagcgt tgatacgaac
      121 aatccacaaa ctacgaaact cgcgtcttct ggccctacgg tcacggtaat tgtaggtcat
      181 gcccttctca accgtttcgc gggcggaacg gtagaggctg tttttgccgc cgtaaaagcc
      241 cttggcggcc ttcaagtatt tatttcgacg gtgacgcgtg acgaccggcg aatgtgatct
      301 tggcatactt cctccagcag gaacagtaaa ggataatggg ttaggcgctc agcatacgct
      361 tgatcctggg ctcatccgcg ggggtggcga tcgcgttttg cctcaggctg cgtttgcgtt
      421 ttgccgactt tttggtcagg atgtggcgcg cgtaattctt gttgcgccgc accttgcccg
      481 ttcccgtgag gctaaagcgt ttcttagcgg cacggttgct cttcattttg ggcatagaat
      541 gctcacctct tttggttgtg ttggctgttt atttcttgac gaaaaagacc acgagctggc
      601 cgccttccat gacgggatct ttttcggttt tggcgatatc ctggagtcct tcgatgaagc
      661 gttccaggat ttttcgacca aactcctgat gaatcatttc gcgtcccttg aactggaccg
      721 aagcacggac gcggtccccc tcgaggagga atttgcgggc gtgcttgatc ttgaattcaa
      781 agtcgtgctc ttcaatcttg ggccgcatgc ggatctcttt ggtggagatg acgtgctgtt
      841 tcttgcgcgt ctccttctcc tttttggaaa gctcgtactt gaacttgccg aaatccataa
      901 ttttacagac cggcggagct gaattgggag agacctcgac cagatccagc cctgcgctct
      961 ccgccctccg cagtgcatca ctgcggctca ccacaccgac ctgttcgccg ttttcgtcga
     1021 ccagtcggac ggaaggagca tcgattagct cattgacgcg agtgagtttt ttcttaatag
     1081 cctgtcctcc tctaatgatt ctacgctttg gcgagaactt tttcctgaat ttccgaaatc
     1141 acagccggga tttcagccaa tccgcaatcc ccttcgcgat gtttacggac ggagacctgg
     1201 ttttgttccg cttctttttg tccgacgatc agcatcacgg ggattttctg ggtttctgct
     1261 tcgcgtattt tataaccaac cttttcgctg cgatcatcca gttcggcgcg gatgccggca
     1321 gcggtgagtt ggtccatgat ttttctcgca tagtcgtgct gattctccgt gatcggcagc
     1381 acgcgcacct gggttggcgc cagccaaagt ggaaaagccc cggcatagtg ctcgatcatc
     1441 actccgaaga agcgttcgag tgagccgagc agggcgcggt gaatcatgat cgggcggtgc
     1501 tccttgccgt cctggccgac gtaggtgagg tcgaagcgtt cgggttcgtt gaaatccacc
     1561 tggatggtgc tgcactgcca ggtgcggccg atggaatccc tgattttgat atcgatcttg
     1621 gggccgtaaa agaccccttc gcccggatcg atctggtaat tgagggccgc tttttccagc
     1681 ccggcgcgca gggcatcggt ggcgcgctcc cagttctccg gcgtaccgac atagtgttcg
     1741 ggccgggtgg agagatagat ctcgaactcc gaaaagccga aggagcgcag gatgaagagg
     1801 gtgaaatcga gcagcatgtc gatttcagcg tcgagctgat cgggacggca aaagtgatgg
     1861 gcatcgtcct gggtgaagcc gcgtacgcgc atcagtccgt gcaaaactcc cgagcgctcg
     1921 tagcggtaga ccgtacccag ttccgcccaa cggatgggca ggtcgcggta actgcgggtc
     1981 tggctgcgga agatctgcag atggaaaggg cagttcatgg gcttgatctg gtagagctgc
     2041 tcgtccacct cggtcggagc gtacatattc tccttgaaaa agccgagatg gccgctggtg
     2101 ttccagagat cgaggcgtgc gatatggggc gtataaacaa actcgtagcc gtttttaaga
     2161 tgttcctggc gccaaaactc ctccaccttg tagcggataa agcctccttt gggatgccag
     2221 aggatgagcc ccgggccgat ctcgtcattg acactgtaga gatcgagctc acggccgaga
     2281 cggcggtggt cgcgcttttt ggcttcctcg atgcgcagga gatagtcgtc gagctgggac
     2341 tttttcgggt aggccacccc gtagatgcgc tgcagcatcg gattcttctc gtccccgagc
     2401 caataggccc cggccacgct catcagcttg aaataacgga catagccggt ggagggcaga
     2461 tggggtccgc ggcagaggtc gataaaatcg ccatcgcgat agatgctcgg gggttcgccg
     2521 aggcgctcga tctgctgaag cttgtagccc tccttgcgtt cgcggaagag gcgcagggcc
     2581 tcctccttgt ccatctcctc acggatgaag gggtggtcag ccgcaacgat ctcggccatc
     2641 tttgcctcga tgcgctgcag atgctcgggt gtaaagctct ccttgacgtc gatatcgtag
     2701 taaaagccct cctcgaccgg ggggccgaag gcgaactggg cctccgggta gagctgcttc
     2761 accgcatggg ccatgatatg ggcagcgctg tgccaaaaga catccatccc ctcgggagag
     2821 tcggcggtaa ggaacaggat gcgcccgctc ctggtcaggg gcctggagag atcaagcact
     2881 tcggcgtcga gtttggcagc aatcgcctca cgggcgatcc ggccgcccag ttcggagagc
     2941 agctcgctcg ctttggtgcc ttcggggtac tcccgtgaac gtccatcggg aaatgtcaga
     3001 gtgatcatgg ccaaacggtg accctttccg aaaagtgatc tcccaaaaca agaaaccgcc
     3061 catggaaggc ggcatctgta agaggaggta atagcgcttg gtactcaaaa gtgggcggta
     3121 ctggatttga accagtgacc ccttgcatg
//
LOCUS       DAOPWU010000380         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_31716_length_3143_cov_3.748381, whole genome shotgun sequence.
ACCESSION   DAOPWU010000380 DAOPWU010000000
VERSION     DAOPWU010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31716_length_3143_cov_3.748381"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..2886)
                     /locus_tag="PKM23_13965"
     CDS             complement(55..2886)
                     /locus_tag="PKM23_13965"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNY92625.1"
                     /translation="MADPLLFSGELPYRQRCEKILALLGVNPKAGLTAAEARQRLEKY
                     GPNELPQEPPVPKWKKFFAQFRDPLTLLLLVATVISFIAWVIERDSVIPFEAITILIV
                     VILNGVLGYLQEERAEQAIAALAAMSAATARVLRDGAQQTLPARELVPGDILLIEEGD
                     TIPADARVLESVALRVAEAALTGESAPVSKDSAHMEDETGIGDQLNMLFSGTAVTSGR
                     GRAVVVGTGSHTEIGKIAGTLQETAEVSTPLQRELAGVGRMLGIAVIAIAILISATIL
                     IVENLRTWADLVDVLLLAVSLAVAAVPEGLTAITTIVLSLGMERMAKRNVIVRKLNAV
                     ETLGSTTVICSDKTGTLTRNEMTVRKVVTASGEASLTGAGYSPAGRLEQGGEPLAGGP
                     LRNEVRYALRAAHLANNARLVHNSEEQWTIQGDPTEGALLVAARKAGWNELELSERFP
                     RVHEVPFSSERKLMSTAHKDTEKEERFMVVSKGAPDVLLSRCSHERVGEEARELTPER
                     RAAILAAVDKLAHEALRTLGVAFRTMRMDEVTGEMNYDEYENQFVFLGVVGMIDPPRP
                     EAAEAVRVAREAGVRSVMITGDHPATAEAIARELGISGENGRVVTGAELQKMSDDELE
                     GVVLKASVYARVAPDHKLRIVRALKAHGEIVAMTGDGVNDAPALKSADIGLAMGITGT
                     DVSKGAADMVLTDDNFASIVAAVEEGRAIFANIQKFLRYLLSSNIGEVFVMFFGVILA
                     GLIGLHPEAGSAVVAPLLAVQILWINLLTDAAPALALGVDPADHRLMKRPPRDPRTRV
                     ITSDMWLGILLVGAVMGIGTLLVVDYALPGGLIPGSGGVPYAQTMAFTTLVFFQLFNV
                     FNARSGYESAFKKLFSNRWIWRAILLSVLLQVMVIYIPFLQKAFGTVGLGLKDWGVCI
                     LAAGSVVVVREISKLLWPRPERKAAPQGA"
BASE COUNT          550 a          975 c         1050 g          568 t
ORIGIN      
        1 tcggttgatc ggatgcttga cgtttactgg ttgaaaccct gatgccactt cgcctcaagc
       61 cccctgcggc gcagctttac gctccggcct gggccagagc agtttggaga tctccctgac
      121 caccaccacg ctgccggcgg ccagaataca gacgccccaa tccttcaggc ccagccccac
      181 tgtgccgaag gctttctgca ggaaggggat gtagatcacc atcacctgca gcaggaccga
      241 gagcagaatc gcccgccaga tccagcggtt gctgaagagc tttttgaagg cgctttcata
      301 gcccgagcgc gcattgaaga cgttgaagag ctgaaagaag accagggtag tgaaggccat
      361 ggtctgggca tagggcacac ccccgctgcc ggggatcagt ccgccgggca gggcgtaatc
      421 gaccaccagc agggtgccaa tccccatcac cgcgccgacg aggaggatgc ccagccacat
      481 gtcgctggtg atgacgcgcg tgcgcggatc ccgaggcggc cgtttcatga gccggtggtc
      541 ggccggatca acgcccaggg cgagggcggg cgcagcatcg gtaagcaggt tgatccagag
      601 gatctgcacc gccagcaggg gggcgaccac ggccgagccg gcctcgggat gcagcccgat
      661 caggccggcc aggatcactc cgaaaaacat gacaaagacc tcgccgatgt ttgaggagag
      721 cagatagcgg aggaatttct ggatgttggc gaagatcgcc cgtccctcct cgacagcggc
      781 aacgatgctg gcaaaattgt cgtctgtcaa caccatgtcg gccgccccct tggagacatc
      841 ggtgccggtg atgcccatgg ccagaccgat gtcagcgctc ttgagggccg gggcatcgtt
      901 gacaccatcg ccggtcatgg cgacgatctc gccgtgcgcc ttgagggcgc ggacgatgcg
      961 cagcttgtga tccggcgcca cccgggcata aaccgatgcc ttcagcacta ccccttccag
     1021 ctcatcgtca ctcatttttt gcagctcggc gccggtgacc acgcggccgt tttcgccgct
     1081 gatgcccagc tcccgggcga tcgcctcggc cgtggccgga tggtcgccgg tgatcatcac
     1141 cgaacgcacc ccggcctcgc gtgccacgcg cacggcctcg gccgcctccg gccggggcgg
     1201 atcgatcatg ccgactacgc cgagaaagac gaactgattc tcatattcat cgtagttcat
     1261 ctctccggtc acctcatcca tgcgcatggt gcggaaggcg acacccaggg tgcgcagggc
     1321 ctcatgggcc agcttgtcga ccgcagcgag gatggccgcc cgccgctcgg gagtcaactc
     1381 ccgtgcctct tcacccacac gctcatgcga gcagcgcgag agcagcacat cgggtgcccc
     1441 cttgctcacc accatgaagc gctcttcctt ctcggtgtcc ttgtgggccg tgctcatcag
     1501 cttgcgctcg ctgctgaaag gcacttcatg gacgcgcggg aagcgctcgg agagctcgag
     1561 ctcattccag cccgccttgc gcgcagccac gagcagggct ccctcggtcg gatcgccctg
     1621 gatggtccac tgctcctcgc tgttgtgcac caggcgggca ttgttggcca ggtgggcggc
     1681 gcgcagggcg tagcgcactt cgttgcgcag ggggcctccg gcgaggggtt cgcctccctg
     1741 ctcgagccgg ccggccgggg agtagcccgc gccggtgagc gaggcctcgc cgctggcggt
     1801 gacgaccttg cgcacggtca tctcgttgcg ggtcagggtg ccagtcttgt ccgagcagat
     1861 caccgtggtc gaacccaggg tttcgacagc attgagcttg cgcacgatga cgttacgttt
     1921 ggccatgcgc tccatgccga gggagaggac gatggtggtg atggcggtca gcccttcagg
     1981 gacggcggct accgccagcg agacggcaag gagcaggaca tcgacgaggt cggcccaggt
     2041 gcgcagattc tcgacgatga ggatggtagc gctgatgagg atggcgatgg cgatgacggc
     2101 gatgccgagc atgcgtccca ccccggccag ctcgcgctgg aggggggtgc tgacctcggc
     2161 ggtctcctgg agggtgccgg cgatcttgcc gatctcggtg tggctgccgg tgccgacgac
     2221 cacggcgcgg ccgcggccgc tggtgacggc ggtgccgctg aagagcatgt tgagctggtc
     2281 gccgatgccg gtttcatcct ccatgtgggc gctgtccttg ctgaccgggg cgctctcgcc
     2341 ggtcagggcc gcctcggcga cgcgcagggc caccgattca aggacgcggg cgtcggcggg
     2401 gatggtgtcg ccctcctcga tgagaaggat gtcgccggga accagttcac gggcggggag
     2461 ggtctgctgg gcgccgtcgc gcaggacgcg ggcggtcgcc gccgacatcg ccgccagggc
     2521 ggcgatcgcc tgctcggcgc gctcctcctg aagatagccg agcacgccgt tgaggatgac
     2581 cacgatcagg atggtgatcg cctcgaaggg gatgaccgaa tcgcgttcga tcacccaggc
     2641 gatgaaggag atcacggtcg cgaccagcag caggagggtg aggggatccc tgaactgggc
     2701 gaagaatttt ttccatttgg ggacaggcgg ctcctggggg agttcgttgg ggccgtattt
     2761 ttccaaacgc tgccgggcct cggctgcggt gaggccggct ttggggttga cgccgagaag
     2821 ggcgaggatt ttttcacagc gctgccggta gggcagttca ccgctgaaaa ggaggggatc
     2881 agccattaag ggtaccggtt ggtgaggaag gcaggaggcg agcggtccct gcctgccgga
     2941 ttatatgatt tgtctaatat agtttacttt gggcaaataa tcaaaggaga tttttttacc
     3001 agggtacccc ctgattctgg gtaccacttg accgctgtgc gccgtcccct gcagaaatct
     3061 ggttcgcatc cggcaccttg taccgcgtac cctgtaaaaa tctcgttccc atccaacacc
     3121 ttgtgccacg taccctgtaa aaa
//
LOCUS       DAOPWU010000381         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_31729_length_3142_cov_3.308066, whole genome shotgun sequence.
ACCESSION   DAOPWU010000381 DAOPWU010000000
VERSION     DAOPWU010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31729_length_3142_cov_3.308066"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="PKM23_13970"
     CDS             <1..121
                     /locus_tag="PKM23_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HNY92626.1"
                     /translation="VSPKEIGTLIEVVKEIDPRAFVIVNNVHEVLGEGFRGRI"
     gene            185..1780
                     /locus_tag="PKM23_13975"
     CDS             185..1780
                     /locus_tag="PKM23_13975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY92627.1"
                     /translation="MRVLLLNPPFLPRYSRSHRVPAVAKSGNLFYPHWLALAAAVLKQ
                     RGHTIDLFDCPAEGVELKLLLTLAVRFDPALIVIDSSTPSWYADCETAQALRETLRHS
                     RICMTGHHATFFWRQTFERASAVDYVALGEYEETVADLADALDQGGDLNTIPGLATRA
                     HLREGRPPLRPLNQDLDRLPMVSPIYKEYLEPELYSFNLSAHPFVQIMAGRGCPSNCF
                     FCAYPQISQGGSYRHRTAENIVKEMEWIQKEWKEIRQINFEDDNFGADTAFARRLGEV
                     VRVHKVDLPIFANISASLDPGSLESLRAAGLHNCSVGFDTIRYSLLEKMGKIQTDRSI
                     HDFMAATRRLGILVHGCFLVGFPGESRGSMREAFKWACRIRPDSAQFYPVMPYPGTGA
                     WSFYTEKGYLATASFRDWLTRAGAPRCVLNLPGLTPREIDDFCDHAFVRFHLRPRYLI
                     QKLWQAVQSPREGLRSLRAARLFARYLRSKREQRPEYLPVQRLGRSETWLIMPVIPEG
                     RMQRIESTSGANPPDAGHSDRKR"
     gene            complement(1733..>3142)
                     /locus_tag="PKM23_13980"
     CDS             complement(1733..>3142)
                     /locus_tag="PKM23_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNY92628.1"
                     /translation="GAQEGASGSEKGGVQPVGRVDAEGRAQDAGRPEDAGSGAADFLL
                     AGRTLTLPMFVATLVSTWYGGILGTGEFSYTYGVASWVMFGAPYYIFALIFAFFLAGR
                     VRATGLVTIPDKLGRHYGQPAALLGALLVFLLVTPAPYLLMLGVLAQLIFGVPLPLAL
                     ILTALASLVYLAKGGFRSNVAVNLAEFVLMFTGFILIVGFAFFRFGGLSFLKPSLPAV
                     NLTWHGGNSPQYILVWYFIGLWTLVDPGFHQRCAAARDSATARRGILVSILFWMLFDF
                     LTTTTGLYARAVLPGLENPIFAYPLLAEQLLPAAVKGLFFIGLLATVMSTLSSYAFLG
                     GMTIGNDIAGRILRMRGKEARGAAERWSRIGLLLASIYGIVLALTLPSVVRIWYAVGS
                     ACIPGLLLPLMASYWPGIALRPPFALASMLGGWLLAMVWMVWGLAHGGDYWWGIEPMY
                     PGLLLSFAIGMAGIRRVRP"
BASE COUNT          619 a         1097 c          944 g          482 t
ORIGIN      
        1 cgtcagcccc aaggagatcg gcaccctcat cgaagtggtc aaggagatcg atccccgggc
       61 ttttgtcatc gtcaacaatg tccatgaggt gctcggcgag ggctttcgcg gccgcatcta
      121 ggcggaccgg aggtgcaagc cggggccctc aaccacagga acggataatt tcagcgggaa
      181 tggtatgcgc gtactcctcc tcaatccgcc ctttctaccc cgctacagcc gcagccatcg
      241 cgtgccagcc gtggccaaat cgggcaatct cttctacccc cactggctgg ccctcgccgc
      301 ggccgtgctg aaacaacgcg gccataccat cgatctcttc gactgccccg ccgagggagt
      361 ggaactgaag ctcctcctca ccctggcggt ccggttcgat cccgccctga tcgtgatcga
      421 ctcctccacc ccgagctggt acgccgactg cgagaccgcc caggccctca gagagacgct
      481 gcgccacagc cgcatctgca tgaccggcca ccacgccacc tttttctggc ggcagacctt
      541 tgagcgcgcc tcggccgtcg actatgtcgc ccttggtgaa tatgaagaga ccgtggccga
      601 cctcgctgat gccctcgacc agggcggcga cctcaacacc atccccggcc tggccacccg
      661 cgcccatctc cgggagggcc gccctcccct gcgccccctc aaccaggacc tcgatcgcct
      721 gcccatggtc tcgcccatct acaaggagta cctcgagccc gaactctatt ccttcaacct
      781 ctccgcccac cccttcgtcc agatcatggc cggccgcggc tgcccctcca actgcttctt
      841 ctgcgcctat ccccagatct cccagggcgg cagctaccgc catcgcaccg ccgaaaatat
      901 cgtcaaggag atggagtgga ttcagaagga gtggaaggag atcagacaga tcaatttcga
      961 ggacgataat ttcggagccg atacggcctt cgcccgccgc ctcggcgagg tcgtccgcgt
     1021 ccacaaggtc gacctcccca tcttcgccaa catcagcgcc tccctcgacc cgggcagcct
     1081 tgaatccctc agggccgccg ggctgcacaa ctgttccgtc ggcttcgaca ccatacgcta
     1141 ctccctgctc gaaaagatgg gcaagatcca gaccgaccgg agcatccatg atttcatggc
     1201 ggcgacgcgc cgcctcggca tcctcgttca cggctgcttc ctggtcggct ttcccggcga
     1261 gagccgcggc agcatgcgcg aggccttcaa gtgggcctgc cggatcaggc cggacagcgc
     1321 ccaattctat ccggtcatgc cttacccggg caccggggcc tggagctttt acacggagaa
     1381 aggctatctg gcgaccgcca gcttcaggga ctggctgacc cgcgccggtg caccgcgctg
     1441 cgtcctcaat ctgccgggtc tgacgccccg ggagatcgac gatttctgcg accacgcctt
     1501 cgtgcgcttc cacctccgcc cccgctacct catccagaag ctctggcagg ccgtgcaaag
     1561 cccgcgcgag gggctgcgct ccctgcgcgc cgcccgcctc ttcgcccgct atctgcgcag
     1621 caagcgggag cagcgacccg agtacctgcc ggtgcagcgt ttgggcaggt cggagacctg
     1681 gctcataatg cccgtcatcc ccgaagggcg catgcagcgc atcgaaagca cctcaggggc
     1741 gaacccgccg gatgccggcc attccgatcg caaaagataa cagcagcccc ggatacatcg
     1801 gctcgatccc ccaccagtaa tctcccccat gagccagccc ccataccatc cagaccatcg
     1861 ccagcagcca tccccccagc atcgaggcca gggcgaatgg cggccgcagg gcgatcccgg
     1921 gccagtagct ggccatcagc ggcagcagca gtccggggat gcaggcgctg ccgaccgcat
     1981 accagatccg caccaccgag ggcaaagtca gggcgaggac gattccgtaa atcgaggcga
     2041 ggagcaaacc gatccggctc cagcgctcgg ccgccccgcg cgcctccttc ccccgcatgc
     2101 gcaggatcct gccggcgatg tcgttgccga tggtcatgcc cccgagaaag gcgtagctgc
     2161 tcagggtcga catcacggta gcgagcagtc cgatgaaaaa gagtcccttg accgccgccg
     2221 gcagcagctg ctcggccagg agtggatagg cgaagatcgg gttctccagt ccgggcagca
     2281 cggcgcgggc gtaaaggccg gttgtggtgg tgagaaaatc gaagagcatc cagaagagga
     2341 tggagacgag gatgccgcgc cgggcggtgg cgctgtcccg ggcggcggcg cagcgctggt
     2401 gaaagccggg atcgaccaga gtccagaggc cgatgaaata ccagaccagg atatattggg
     2461 gtgaattgcc gccgtgccag gtcagattga ccgccggcag gctgggtttg aggaaggaga
     2521 ggccgccgaa gcggaagaag gcaaagccga cgatgaggat aaaaccggta aacatgagca
     2581 cgaattcggc cagattgacc gccacattgg agcgaaaccc ccccttggcg agatagacca
     2641 ggctcgccag agcggtgagg atgagagcca ggggcagggg cactccgaaa atgagctgcg
     2701 ccagcacccc gagcatgagc agataggggg ccggagtgac cagcagaaag accagcaggg
     2761 cgccaagcag ggccgccggc tggccgtaat ggcggccgag cttgtccggg atggtgacca
     2821 gcccggtggc gcgcacccgg ccggcgagaa aaaaagcaaa gatcaaagca aagatgtaat
     2881 agggcgcccc gaacatcacc cagctggcga cgccgtaggt gtaggagaac tcgccggtgc
     2941 cgaggatgcc cccgtaccag gtagaaacca gggtggcgac aaacatcggc agggtgaggg
     3001 tgcgcccggc gaggagaaaa tcggcggccc cgcttcccgc atcctcaggc ctgcctgcat
     3061 cttgcgccct cccctctgcg tccacgcgcc cgaccggctg cacaccccct ttttcgcttc
     3121 cactcgctcc ctcctgcgct cc
//
LOCUS       DAOPWU010000382         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_31936_length_3127_cov_4.789714, whole genome shotgun sequence.
ACCESSION   DAOPWU010000382 DAOPWU010000000
VERSION     DAOPWU010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31936_length_3127_cov_4.789714"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="PKM23_13985"
     CDS             complement(<1..1912)
                     /locus_tag="PKM23_13985"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNY92629.1"
                     /translation="MPCFRRIALIGLVMAAAATAASLSIPFEKYQLENGLEVILHRDL
                     SDPIVAVAIQYHVGSNREVRGRTGFAHLFEHMMFQESQHVGQDQFFKKIQGAGGELNG
                     GTGNDGTVYYQVVPRNALEMVLWLESDRMGWLLSTVTQEAFENQQGVVQNEKRQNYDN
                     RPYGQTNYVIGKLLYPPDHPYNWTVIGEMADLRSATLADVHDFYRKWYGPNNATLVVA
                     GDFDPAQARSWIERYFGEIPSRGEVNPLPPMPVQLEASRRVWYEDSFIKSPELTLVFP
                     TMQRFTREGHALQFLAEFLAGSKKSPLYQIVVEEEKLAPAVRAWQSSQEVAGAFYIRI
                     RAFPEVSLARVEEAVTRAFARFEERGFTGRDLQRRKAGMETAWYNRVSSVLGKSMQLA
                     NYNEYAGSPGYIATDLEQARSITGEEVWQAYRTFIKGRPSVLTAFVPRGRSDLAAAGS
                     VCFEIAEDQPAAAPLPGAEQKAPMRVERIPSRIDRAREPEPGPDPVIHSPRVWRKELK
                     NGMQLFGIEQHELPLVQASLTIRGGALLDGEGKSGTANLLAASLMEGTRTRTPVELEE
                     AIEEVGASISISASAENITLNATALAEHWPAVAELIAEILLEPRWDETEFARLRQETL
                     EEIQRSQSSPAAI"
     gene            2161..2640
                     /locus_tag="PKM23_13990"
     CDS             2161..2640
                     /locus_tag="PKM23_13990"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92630.1"
                     /translation="MAELSGSYNITLDTNRRLALPTKLYYSQEDELRDRVKITRGIAK
                     CLTGYYRTEWKALVSSLNKLNIDEKSKQIMKRHIIGAAADCELDKQRRLVLPENLVAY
                     AELPENGEVLVVGCGEVFEIWNPALYQADAVASEETIQEIFGRISLSASQASSPEPD"
     gene            2659..>3127
                     /gene="mraW"
                     /locus_tag="PKM23_13995"
     CDS             2659..>3127
                     /gene="mraW"
                     /locus_tag="PKM23_13995"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNY92631.1"
                     /translation="MEGYHQSVLVREVLELLRAEEARLVYDVTAGDGGHMEAFLQVMP
                     SAGRISGIDRDPQALQRLRQRLASYADRIQVVRGNFRDLQTLLAQSDQGHVDAILADL
                     GISTLQITVPEKGFMFSADGPLAMQMDPQSGLSAETVVNEFDEEQLGEIFRTLG"
BASE COUNT          607 a          883 c          980 g          657 t
ORIGIN      
        1 cgatggcggc agggctggat tggctgcgct gaatctcttc cagggtctcc tgacgcagac
       61 gggcgaactc ggtctcatcc cagcgcggtt cgagcaggat ctccgcgatc agctccgcca
      121 cggccggcca gtgctcggcc agggcggtgg cgttgagagt gatgttctcg gcggaggcgc
      181 tgatcgatat cgatgctccc acctcctcaa tcgcctcctc cagctccacc ggggttctgg
      241 tccgtgtccc ctccatcaga gaggcggcga ggagattggc tgtgccgctt ttgccctccc
      301 cgtccagcag agcgcccccc ctgatcgtca gcgaggcctg caccagaggc agctcgtgct
      361 gctcgatgcc gaagagctgc attccgttct ttaattcctt gcgccagacc cgcggggagt
      421 gaatgacggg atcagggccg ggttcgggct cgcgggcgcg gtcgattctg ctcgggatcc
      481 tctccaccct catgggcgcc ttttgctccg caccgggaag gggcgcagca gcgggctgat
      541 cctccgcaat ttcaaagcac accgagcccg cagccgccag atcgctgcgg ccgcggggta
      601 cgaaggcggt gagtactgaa ggccttccct tgatgaaggt gcggtaggcc tgccagacct
      661 cctccccggt gatcgaccgg gcttgttcca gatcggtggc gatatagccg ggcgatccgg
      721 cgtattcatt ataattggcc aactgcatcg atttaccgag aacacttgag acgcggttgt
      781 accaggcagt ctccatgccc gccttgcggc gctgcagatc ccggccggta aaaccccgct
      841 cctcgaagcg ggcgaatgcg cgagtgaccg cctcctcaac ccgcgcgagg gagacttcag
      901 gaaaagcgcg gatacgaatg taaaaggcgc cggcgacctc ctgactgctc tgccaggctc
      961 gcaccgcagg ggcgagcttt tcctcctcca ccacgatctg gtaaaggggg gattttttgc
     1021 tgccggccag aaattcggcc aggaactgga gggcgtgccc ctcccgggtg aaacgctgca
     1081 tggtcgggaa gaccagggtc agttccgggg atttgataaa gctgtcctca taccagaccc
     1141 ggcggctcgc ctcgagctgg accggcattg gggggagggg atttacctcc ccgcgggagg
     1201 gaatctcgcc gaaatacctc tctatccacg atcgggcttg cgcgggatca aagtcgccgg
     1261 cgaccaccag ggtggcgtta ttggggccgt accatttccg gtaaaagtca tgcacatcgg
     1321 ccagggtcgc gctgcgcaga tccgccatct cgccgatcac cgtccagtta tagggatgat
     1381 cggggggata aaggagcttg ccgatgacgt agttggtctg cccgtagggg cggttgtcgt
     1441 aattctgccg tttttcattc tggaccacac cctgctggtt ctcaaaggcc tcctgcgtga
     1501 ctgtgctcag cagccagccc atgcgatctg attccagcca caggaccatc tccagggcat
     1561 tgcgcggcac cacctgataa tagaccgtgc cgtcgttgcc ggtgccgccg ttgagctcgc
     1621 ctccggcccc ctgtatcttt ttgaaaaact ggtcctggcc cacgtgctgg gactcctgga
     1681 acatcatgtg ctcgaagagg tgggcgaaac cggtgcgtcc gcgcacctcg cggttggagc
     1741 cgacgtggta ctggatggcg acggccacta tgggatcgga gagatcccgg tggaggatga
     1801 cctccaggcc attctcgagc tgatactttt caaaggggat ggagagggag gccgcggtgg
     1861 cggcagcggc cataacaagg ccgatcaggg ctatacggcg gaagcacggc atgggtgatc
     1921 tccctgcgtc atgggtatgc ggcagtggga ctctggtcga ttataaacgg gattggtcat
     1981 cagcggcctg gcgggacgtt tctctgaggc acaaaaaaac aatataaaat cgcttttttt
     2041 acttgatttt taatagctaa taagctatct tatggtgaag aatggagatt tttagaaata
     2101 attggattct attggatttt tttggtgtta aatcctctct ttatcggggt cctctgaatc
     2161 atggccgaac tctcaggctc atataacatc accctggata ccaaccggcg gctggcgctg
     2221 ccgaccaagc tttactattc ccaggaagat gaactgcgtg accgggtcaa gatcacccgt
     2281 gggatcgcca agtgcctgac cggctactac cggaccgaat ggaaagccct ggtctcctcc
     2341 ctcaataaat taaatattga tgagaaatcc aagcaaatta tgaagcgcca tatcatcggc
     2401 gcggctgcgg attgtgaact ggacaagcag cggcggctgg tcctcccgga aaatctggtc
     2461 gcctatgcgg agcttcccga gaacggcgag gtcctggtag taggctgcgg tgaggtgttc
     2521 gaaatctgga atccggccct ctatcaggca gatgccgtgg ccagtgagga gaccatccag
     2581 gagatattcg gacgtatttc gctcagcgca agtcaggcct catccccgga gccggactga
     2641 gcggcggggg ggagagcagt ggaaggatat catcagtcgg tactggtccg ggaggttctg
     2701 gagctgctca gggccgagga ggcccggctg gtgtatgatg tgacggccgg cgacggtggg
     2761 cacatggagg ctttcctcca ggtgatgccg tccgcgggac ggatttcagg catcgacagg
     2821 gatccccagg ccttgcagag gctgcggcag cggctggcca gttatgcaga tcgtatccag
     2881 gtagtgcggg gtaattttcg cgatctacag actcttctgg cgcaatctga tcagggtcat
     2941 gtagatgcca ttctcgccga cttgggtatt tccaccctgc aaatcaccgt tccggagaag
     3001 ggttttatgt tttcagcgga tggaccgctt gcgatgcaga tggatccaca gagcggtctc
     3061 agcgccgaga cggtcgtcaa cgagtttgac gaggagcagt tgggcgagat cttccgcacc
     3121 ctgggtg
//
LOCUS       DAOPWU010000383         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_31938_length_3127_cov_4.289388, whole genome shotgun sequence.
ACCESSION   DAOPWU010000383 DAOPWU010000000
VERSION     DAOPWU010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31938_length_3127_cov_4.289388"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PKM23_14000"
     CDS             complement(<1..460)
                     /locus_tag="PKM23_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92632.1"
                     /translation="MKHSGFILFLSIALILYTWMNWYLIRRGLQALPGPSGLRSAFTI
                     LIILLAAAYPLGRLCERSLNTGLGAFLVHIGAYYTAVLIFALLALIIIDLVRLADHFF
                     SFLPAAWHEARSSARTLLLAGVAALTLILTLAGAINARDPRVRHLDIAIDK"
     gene            627..2111
                     /gene="dacB"
                     /locus_tag="PKM23_14005"
     CDS             627..2111
                     /gene="dacB"
                     /locus_tag="PKM23_14005"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNY92633.1"
                     /translation="MNPKILALLCLLLCTAAVLAGPPLDDSQEALIKIRNDLEYIFSD
                     PSFSAAHWGVAVQSTLTGEYFYLRNENKAFMPASNMKLFTTAAGLLGLGPDFRYVTRL
                     LAHGLISAEGVLEGDLIIRGCGDPSLSGRWENGKITAVFERWADSLQARKITAITGKV
                     IGDGRYFSDGRIGAGWAWDDLTDYYSAQISALTFNDNCVDLIFMPGDSLGAAARYRLE
                     PNTAYVQIVNRVTTGSRSKLGFWRDPGSNKIICEGTISQGSKERRDWITVENPAKYTA
                     TVLREILLSRGIRVYEEAFEIDELPGYLPVEYKTVQLASHTSPPLSEIVHKINKDSNN
                     LFAELLLRTVGRELEGSGSAEAGEKAAKQLFEPAGITPQQFYAADGSGLSRQNLVSPM
                     SIILLLRHMREQPAGQAYYDSLPVAGVDGTLKGRMRGTAAKGNVRAKTGLIGRVRALS
                     GYVTTRDNELLVFSMIVNNYNVPTGTANHLQDLVCERLANFTRK"
     gene            2172..>3127
                     /locus_tag="PKM23_14010"
     CDS             2172..>3127
                     /locus_tag="PKM23_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNY92634.1"
                     /translation="MMGSGSRHIFRWFESAPLRVKLILTIGLLFIGLMSVLSFVVIQQ
                     GKSILNERLAETCTMVMQHVSTAIKDDLLLYYRTDMDANSSSLQLGHIRESILSVYNE
                     NIAGLAYVGVIDRSGIIIAHTNTERLYRKLTSADSSWMLALESTMVRERGANIEYLHP
                     MYTRRESAGDRRIFLGITVLGFDKDIIMRPIQQVTRAILSATALVIIISIVIIFIIAR
                     RMTAQIELLGGGLRQVTGGNLHIQIPVLATDELGRLTMEFNAMIVQLREKLQMQKFVS
                     KLTLQMIRSTANGDLRSATAGQKRVITVLFSDIRSFSAMTERL"
BASE COUNT          627 a          945 c          943 g          612 t
ORIGIN      
        1 gtttgtcgat ggcgatgtcg aggtggcgca cgcggggatc gcgggcgttg atggcaccgg
       61 ccagggtcag gatgagggtg agggcggcga cgccggccag gagcagggtg cgcgcgctgc
      121 tgcgggcctc atgccaggcc gccggcagaa aggagaagaa atggtcggcg agccggacga
      181 gatcgatgat gatgagggcg agcagtgcga aaatcagcac ggccgtataa taggcgccga
      241 tgtggacgag gaaagcgccc aagccggtat tgaggctgcg ttcgcagagc cggcccaggg
      301 ggtaggccgc tgcgagcagg atgatgagga tggtgaaggc gctgcgcagc cccgatgggc
      361 ccggcagggc ctgaaggccg cgccggatga ggtaccagtt catccaggtg tagaggatga
      421 gggctatact gagaaagagg ataaatccgc tgtgtttcat cagtgttgag gctctcggtt
      481 aaattaattt ccgcaaattt acacattttt gcatataaaa gaaagagaat cctcctctct
      541 tctgacacgg ccataaaagg cttgattctt tggtgaattt tctctaattt cagtaaattg
      601 attttcttat cctgggtaga tccaacatga atccgaaaat cctggcgctt ctctgtctgc
      661 tcctctgcac tgctgccgtc ctcgccggtc cgcccctcga tgactcgcag gaggccctga
      721 tcaagatccg caacgacctg gagtacatct tttcagatcc ctcgttcagt gcggcccact
      781 ggggcgtcgc ggtgcagtcc accctgacgg gtgaatattt ctatttgcgc aacgaaaaca
      841 aggctttcat gcccgcctcg aacatgaagc ttttcaccac cgccgccggt ctgctcggtc
      901 tggggcccga tttccgctac gtcacccggc tgctggcgca cgggctgatt tcggcggagg
      961 gggtgctgga gggggatctg atcatcaggg gctgtggcga tccctcgctg agcgggcgct
     1021 gggagaatgg caaaatcact gcggtctttg agcgctgggc ggacagcctg caggcgcgca
     1081 aaatcaccgc catcaccggc aaggtcatcg gcgacggccg ctacttcagc gacggccgca
     1141 tcggagccgg ctgggcctgg gatgacctca ccgattacta ctccgcccag atcagcgccc
     1201 tgaccttcaa tgacaactgc gttgacctca tcttcatgcc tggcgacagc ctcggcgcag
     1261 ccgcccgcta ccgtcttgag cccaataccg cctatgtgca gattgtcaac cgggtgacga
     1321 cgggctcgcg cagcaagctt ggtttctggc gcgatccggg cagcaacaag attatctgcg
     1381 agggcacgat cagtcagggc agcaaggagc ggcgcgactg gattacagtg gaaaatccgg
     1441 ccaagtatac ggccacggtg ctgcgcgaga ttctgctcag ccgcggaatc cgcgtctatg
     1501 aggaggcctt tgaaatcgac gagttgccgg gctacctccc ggttgaatac aagaccgtgc
     1561 agctggcctc gcacacctcg ccgcccctga gcgagatcgt ccacaagatc aacaaggaca
     1621 gcaacaacct cttcgccgag cttcttctgc gcacggtggg ccgcgagctc gagggcagcg
     1681 gcagcgccga ggccggcgaa aaggcggcca agcagctctt tgagcccgcc gggatcacgc
     1741 cgcagcagtt ctacgcggcc gatggttccg ggctttcccg ccagaacctg gtctcgccga
     1801 tgagcatcat tttgctgctg cgtcatatgc gcgagcaacc ggccggccag gcttattacg
     1861 acagcctgcc ggtggccggc gtcgacggca ccttgaaggg gcgcatgcgc ggaacagcgg
     1921 ccaagggcaa tgtaagggcc aagaccggtc tgatcggacg cgtacgcgcc ctttccggct
     1981 atgtgaccac ccgtgacaac gagctgctgg tcttttccat gatcgtcaac aactataacg
     2041 tgccgaccgg cacggccaac cacctgcagg atctggtttg cgagcgcctc gccaatttca
     2101 cccgcaaatg accggcgctg ccgcctgcac cgcctggaca cgacagcctg aatgaccctt
     2161 gggcgagtag gatgatgggt tctggatcgc gacatatctt ccgctggttc gagtcagccc
     2221 ccctgcgggt caagctgatc ctgaccatcg gactgctttt catcggtctg atgtcggtgt
     2281 tgagttttgt cgtcatccag cagggcaaga gcatcctcaa cgaacgtctc gccgagacct
     2341 gcaccatggt catgcagcac gtctccacgg cgatcaagga tgacctgctc ctctattacc
     2401 gcaccgacat ggacgccaac agcagttcgc tccagctcgg ccacatccgg gagtcgatcc
     2461 tctcggtcta caacgagaac attgccggcc tcgcttacgt cggtgtgatc gaccgctcgg
     2521 gcatcatcat cgcccacacc aacaccgaac ggctctatcg caagctcacc tcggcggaca
     2581 gcagctggat gctcgccctc gagtcgacca tggtgcggga gcgcggtgcc aacatcgagt
     2641 atctgcatcc catgtacacc cgccgcgaga gcgccggcga ccggcggatc tttctcggca
     2701 tcacggtgct gggatttgac aaagatatta tcatgcgtcc gatccagcag gtcacccggg
     2761 cgattctttc agccacagcc ctggtgatca tcatctcgat cgtgatcatt tttatcatcg
     2821 cgcggcggat gaccgcccag atcgagctgc tcggcggggg gctgcgccag gtcaccggcg
     2881 gcaacctcca tatccagatc ccggtcctgg ccactgacga gctcggccgg ttgaccatgg
     2941 agttcaatgc catgatagtg cagctgcgtg aaaagctgca gatgcagaaa ttcgtctcaa
     3001 aattgaccct gcagatgatc cgctccaccg ccaacgggga ccttcgttct gccacagcgg
     3061 ggcagaagcg ggtgatcacg gtcctttttt ccgatatccg cagcttttcg gcgatgaccg
     3121 agcggct
//
LOCUS       DAOPWU010000384         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_32211_length_3110_cov_5.318494, whole genome shotgun sequence.
ACCESSION   DAOPWU010000384 DAOPWU010000000
VERSION     DAOPWU010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32211_length_3110_cov_5.318494"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3110)
                     /locus_tag="PKM23_14015"
     CDS             complement(<1..>3110)
                     /locus_tag="PKM23_14015"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92635.1"
                     /translation="AAQTSAALQEAAEVMVKAEESYARAIDRGLEEWAAQQVLNTRME
                     EALRPEKMVPMFLRGFRSLQEAKAGRPALAKLASAPTVESGISGQILVDGEAPAYAVT
                     VVAFDANGYYRGDSDDHFDSSTGSYSILSLPPGEYYLLTVSDRYVDEIYNNLTLPLDA
                     RETWRLAEKVTVTENQMTAGIDFDLQKGAELSGIIMREDGMPVQGMTLSFVFTLPNSP
                     VAVFTVETTTDDGFFILHVPLVGEYKLSVKVVDETSQQTWYPNQAAWALGETVVLSGY
                     EASVSDLIFTLAPWPAGSKPGSISGIYRWSDTDQLISIATAFLFHAEDSSLADFKLGL
                     LGSYFFPEVKPGEYILYLDDQLGNLIGGANYLGQFYSGARTPGAATRITVGEGEEVVL
                     NDIVLEPGGTISGTIKSAEGKPLNDIWVLAIDASLMELRLEPWLNNLHLFIGKADARG
                     AYAIAGVPTGHYLVRTISDTLINQNLLNLIKITSGAHAGEVVDAWYGGAANLFSAGSA
                     TPVEVTAPLTTAGIDFTLEKGKWIRGRVTDSINGAGKSFFRLFALDDSTALPYLSLGM
                     LIHDNQMDDQGYYRLGPLPAGTYKILAMAPFTGFNNYLSEMYGGARSFASAEVVNVGG
                     GDLSGLDIKVDRGAIIQGFIDLPQEGGGTVRAGADILDGFPVMVYEAASGKLASMDFV
                     QFNGGYRIDRLLPGTYKILALPAVAPYAATWFGGGGAFASAAEVTVGYGETLVCDILI
                     EKGGGAIAGSILDNGSGAPLSQVMVIAYDATGHPAGLSMSDMDMATGRLISQTGQFTI
                     PGLRAGDYYLRTFALSTALGLTGQFTGLLDLFSPGSGEEIDYMGLLFGSGLGDLSGLF
                     DLNMTVYGDQWYRTAPAQIEINLDGLVLNMLAYGTPSGYDEAMLPIFLPLPLAEKIPA
                     SALAVTVADGATVSGMEFRLSEAGLGDVISDVEEKPAALPARFTVHANYPNPFNPSTT
                     LGFELPAASHLRVEIYNALGHRVRLLHEGTLPAGAHQLIWDGRSDAGEAAPTGLYFAR
                     FQSGND"
BASE COUNT          610 a          932 c         1009 g          559 t
ORIGIN      
        1 atcattcccg gactggaagc gggcgaagta gaggcccgtt ggagccgcct cacccgcatc
       61 gctgcggccg tcccagatga gctggtgcgc tccggccggg agggtgccct catggagcag
      121 gcgcacgcga tgccccaggg cgttgtagat ctcgaccctg agatggctcg ccgccggcag
      181 ctcgaaaccg agcgttgtcg agggattgaa agggttggga tagttggcat ggaccgtgaa
      241 gcgagcgggc agcgcggccg gcttctcctc cacatccgaa atcacatccc cgagtcccgc
      301 ttcgctcagc ctgaattcca tgcccgatac ggtggcgccg tcggccacag tgaccgccag
      361 tgcggaggcc ggtatctttt cggccagggg taggggcaga aagatgggca gcatggcctc
      421 atcatatccg ctcggggtgc cgtaggcgag catgttgagg accaaaccat ccagattgat
      481 ctcgatctgc gccggggctg tccggtacca ctggtcgcca tagacggtca tgttgagatc
      541 gaaaaggccg ctcagatcgc cgagtccgga gccgaagagg aggcccatgt aatcgatctc
      601 ctcgccgctg ccgggtgaga agaggtcgag caggcccgtg aactggccgg tgaggccgag
      661 ggcggtactg agagcgaaag tgcgcaggta atagtcgccc gcgcgcagtc cggggatggt
      721 gaactgcccg gtttggctga tcagacgccc ggtggccata tccatgtcac tcatgctcag
      781 tccggccgga tgaccggtgg cgtcgtaggc gatgaccatg acctgcgaga gaggggcgcc
      841 ggaaccgttg tcaagtatgg agccggcgat ggcgccgcca cccttttcga tgaggatgtc
      901 gcagaccagg gtctcgccgt aaccgacggt aacctcggcc gcactggcaa aggcgccccc
      961 gccgccgaac caggtcgccg catagggggc cacggccggc agggcaagga tcttgtaggt
     1021 gccgggcagc agccggtcga tccggtagcc gccgttgaac tggacaaaat ccatgctcgc
     1081 cagcttgccg ctcgccgcct catagaccat caccggaaag ccgtccagaa tatccgcgcc
     1141 ggcgcggacg gtaccgccgc cttcctgcgg gagatcgatg aagccctgga tgatggcgcc
     1201 gcggtcgacc ttgatgtcca aaccgctcaa atcacccccg cctacattga cgacctcggc
     1261 gctggcgaaa ctgcgcgctc cgccgtacat ctcgctgaga tagttattaa agcccgtgaa
     1321 gggggccatg gcgaggatct tgtaggtgcc ggcgggcaga gggccgagcc tgtaatagcc
     1381 ttgatcgtcc atctgattgt catggatgag catgccgagc gagagatagg gcagggcggt
     1441 gctgtcatcc agagcaaaga ggcggaaaaa gctcttgccg gcgccattga tgctgtcggt
     1501 cacccggccg cggatccatt tgcccttttc aagggtgaaa tcgatgccgg cggttgttaa
     1561 gggggcggtg acctccaccg gggtggcgga acccgcgctg aagaggttgg ccgcgccgcc
     1621 ataccaggcg tccaccacct cgccagcgtg ggcacccgag gtgatcttga tcaggttgag
     1681 caggttctga ttgatcaggg tgtccgaaat ggtgcggacg agataatgcc ccgtcggcac
     1741 gccggcgatc gcataggccc ccctggcgtc agccttgccg atgaaaaggt ggagattgtt
     1801 cagccagggc tccagcctga gttccatcag ggaggcatcg atggcgagca cccagatgtc
     1861 attgaggggt ttgccctccg ccgatttgat cgtgccggag atggtgccgc cgggctcgag
     1921 cacgatgtcg ttgaggacga cctcctcccc ttcaccgacc gtgatccggg tcgctgcgcc
     1981 cggggtccgg gcacccgaat agaactggcc gaggtaattg gccccgccga tgagattgcc
     2041 gagctgatca tcgagataga ggatgtattc accgggtttg acctcgggga agaaatagct
     2101 gcccagcaga cccagcttga aatccgccag cgaggaatcc tcagcgtgga acagaaaggc
     2161 ggtggcgatg ctgatgagct ggtcggtgtc actccagcgg tagatccccg aaatggatcc
     2221 cggcttgctg ccggccggcc agggcgccag ggtgaaaatg aggtcgctga ccgaggcctc
     2281 atagccggaa aggacgacgg tctcgcccag ggcccaggcc gcctggttcg gataccaggt
     2341 ctgctgcgaa gtctcatcaa ccacctttac agagagcttg tattcaccca cgaggggcac
     2401 atggagtatg aaaaaaccat catctgtggt ggtctcgacg gtgaagaccg cgaccgggga
     2461 attcggcagg gtgaagacga atgagagggt catgccctgg acgggcatgc cgtcctcacg
     2521 catgatgatg cccgaaagct cagcaccctt ttgcagatca aaatcgatgc ccgcagtcat
     2581 ctggttctct gtcactgtga ccttttcggc caggcgccag gtctcccggg catcgagggg
     2641 cagggtcagg ttattgtaaa tttcatcgac atagcggtcg ctcacggtca gaaggtaata
     2701 ctcacccggc ggcaggctca ggatcgaata actgccggtg gaggaatcga aatggtcatc
     2761 gctgtccccc cggtaatagc cgttggcatc aaaggcgacc accgtgacag catacgcggg
     2821 ggcctcgcca tccacaagaa tctgtcctga aattccggat tcaaccgtcg gcgcagacgc
     2881 gagtttggcc agggcaggcc ggccggcttt cgcctcctga aggctgcgga agccgcgcag
     2941 aaacatcggg accatctttt ccggcctcaa ggcttcctcc atgcgagtgt tcagcacctg
     3001 ttgtgcggcc cattcctcca gcccgcgatc gatggcgcgc gcataggact cttcagcctt
     3061 gaccatcacc tccgccgcct cctgcagcgc tgccgaggtc tgcgccgcct
//
LOCUS       DAOPWU010000385         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_32372_length_3098_cov_4.480776, whole genome shotgun sequence.
ACCESSION   DAOPWU010000385 DAOPWU010000000
VERSION     DAOPWU010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32372_length_3098_cov_4.480776"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..2299
                     /locus_tag="PKM23_14020"
     CDS             146..2299
                     /locus_tag="PKM23_14020"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNY92636.1"
                     /translation="MEEVYQKVFEGLVRRIRRYNQSMDVALLRKAFEFSYEAHRNQLR
                     KSGHKYFEHPLEVAKILTGLKMDYETIAAAILHDVAEDTEYNIARVESEFGHSIASLV
                     DGVTKISDIKLLEFEERQAENFRKMLLSMVKDIRVIIIKFADRLHNMRTLEFLPEKKR
                     RRIALETREVYAPLAHRLGLDKIKWELEDLALKFLEPEIYSDLVKRVNATRRERELII
                     SHVTKPVREALVEARIHARFEGRPKHFYSIYNKIVKRGIVFEEIYDLHAIRIIVEKVE
                     ECYHTLGIVHSLYTPIHERFKDYIAMPKSNGYQSLHTTVIGPDGKKVEIQIRTESMHR
                     TAEDGIAAHWRYKEGRLQEDELDKHMGWLRQVLEAVDEEEVETSSFMEHLKVNLFQDE
                     VFVFTPRGDLYRLPVHSTPVDFAFAVHTDIGLHCLAAKVNGRIVPLSHQLVSGDTVEI
                     ITSANQRPNEGWLSFATSAKAKGRIKKWLRDTQFESSARLGEEMLTRALSKYSIKFKD
                     VNLADLASKFSQRDAQQLLAALGRGEIKLDAVLHKIIPEDKPVTNNESLFKRFIERAR
                     KSSKGVSVAGMDNIMISFGKCCNPVPGDPITGIVTRGRGIVVHTNSCNNLLRLMQEPE
                     RIIDVAWDVDKENRFLAGLYILCERRSRFLADLSEAVSSSDGIITSANMNADISLVNC
                     TVSIEVYDLDHLNRIIAKIKKVAGVISVSRWSEPK"
     gene            2458..2793
                     /locus_tag="PKM23_14025"
     CDS             2458..2793
                     /locus_tag="PKM23_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNY92637.1"
                     /translation="MADEALKSTITKKDVAKRTAKIVGEKIYLTEKVVDGVFQALREF
                     MSEANPEVRIEIRDFGVFEVKKTKPKPKARNPKTGEIIYVPARRKTHFKAGKLLKEVL
                     KQPLPLESK"
     gene            2827..>3098
                     /locus_tag="PKM23_14030"
     CDS             2827..>3098
                     /locus_tag="PKM23_14030"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvX/YqgF family protein"
                     /protein_id="HNY92638.1"
                     /translation="MADLTPIPVEQLPQGRIVGIDYGERRIGLAVSDPLQTIAFPLVT
                     LFRKGQAFPVEQIIASVQEQAAVALVVSLPLHMDGSLGDKAQAAEA"
BASE COUNT          739 a          925 c          819 g          615 t
ORIGIN      
        1 atcgccaagc agcgcaacgg tcccaccgac agcgtgaact gttactttta caaggaatat
       61 ctccttttcg ccggcaagga atcccggcag cctgaaatcc agaccatttc accgactcct
      121 tactgacatc caggcgcggc agagcatgga agaagtctac caaaaggtat tcgaaggact
      181 cgtacgccgc atccggcgct acaatcagtc catggatgtg gcgctgctgc gcaaggcctt
      241 cgagttcagc tatgaggccc accgcaatca gctgcgcaag tcaggccaca agtacttcga
      301 acaccccctc gaggtcgcca agatcctcac cggtctgaag atggattatg agaccattgc
      361 cgcggccatc ctccatgacg tcgccgaaga caccgaatac aatatcgccc gcgtcgagag
      421 cgagttcggc cacagcatcg ccagcctggt cgacggggtc accaagatct ccgatatcaa
      481 gctgctcgag ttcgaggagc ggcaggcgga gaattttcgc aagatgctcc tctccatggt
      541 caaggacatc cgggtgatca tcatcaagtt cgccgaccgt ttgcacaaca tgcgcaccct
      601 cgagttcctt cccgagaaaa agcggcgccg catcgccctc gaaacccgcg aggtctatgc
      661 ccccctggcc caccgtctcg gcctcgacaa gatcaaatgg gagctggaag accttgccct
      721 caagtttctc gagccggaga tttacagcga tctcgtcaag cgggtcaacg caacccggcg
      781 ggaacgcgag ctcatcatca gccacgtcac caagccggtg cgtgaagccc tggtcgaggc
      841 cagaatccac gcccgcttcg agggccggcc gaaacacttt tacagcatct acaacaagat
      901 tgtcaagcgc ggcatcgtct ttgaggagat ctacgacctc cacgccatcc gcatcattgt
      961 cgaaaaggtg gaggagtgct accacaccct cggcatcgtc cattccctat acaccccgat
     1021 ccatgagcgt ttcaaagact atatcgcgat gcccaaaagc aacggctatc aatcccttca
     1081 taccaccgtc atcggcccgg atggcaaaaa agtcgagatt cagatccgca ccgagtcgat
     1141 gcaccgcacc gccgaagacg gcatcgccgc ccactggcgt tacaaggagg ggcgcctgca
     1201 ggaggatgag ctcgacaagc acatgggatg gctgcgtcag gtgctcgagg cggtggacga
     1261 ggaggaggtc gaaaccagct ccttcatgga gcatctcaag gtcaatctct tccaggatga
     1321 ggtctttgtc ttcaccccgc gcggggacct ttaccgcctg ccggttcatt ccacgccggt
     1381 cgactttgcc tttgcagttc atacagacat cgggctgcac tgcctcgccg ccaaggtcaa
     1441 cggccgcatc gtccccctga gccatcagct ggtcagcggt gatacagtgg agatcatcac
     1501 ctctgccaac cagcggccca acgagggctg gctcagcttc gccaccagcg ccaaggccaa
     1561 ggggcgcatc aagaaatggc tgcgcgatac ccagtttgag agcagcgccc ggctggggga
     1621 ggagatgctg acccgcgccc tcagcaagta ctcgatcaag ttcaaggatg tcaatctggc
     1681 tgatctggct tcgaaattca gccagcggga cgcccagcag ctcctcgccg ccctgggccg
     1741 cggagagatc aagctcgatg ccgtattgca caagatcatc ccggaagaca agccggtcac
     1801 caataacgaa tcccttttca aacgcttcat cgagcgggcg cgcaagtcga gcaagggggt
     1861 gagcgtggcc gggatggaca acatcatgat cagcttcggc aagtgctgca atcccgtgcc
     1921 gggggatccg atcacaggga tcgtcacccg cggacgcggg atcgtcgtgc acaccaacag
     1981 ctgcaacaac ctgctgcgtc tgatgcagga gcccgagcgc atcatcgatg tcgcctggga
     2041 tgtcgacaag gaaaatcgct ttcttgccgg gctctacatc ctttgcgagc gccgcagccg
     2101 ctttctcgcc gacctctcgg aggcggtctc cagttccgac ggcattatca ccagcgccaa
     2161 tatgaatgcc gatatctccc tggttaactg caccgtcagt attgaagtat atgatcttga
     2221 tcatcttaac aggattattg ctaaaatcaa aaaggtggcc ggagtgatct ccgtcagccg
     2281 ctggtcagag cctaagtgaa tgaaaaaaat gcattttttt tgtaatttta tgaccttcca
     2341 tgcatttttt tgttgtcttt tatctaaaaa atagttattt atggttatta attatagtgt
     2401 tatactgtgg acgacaataa ctatacctat ttcaaaaccc aatcttagga gctggccatg
     2461 gctgatgagg ctttgaaatc cacgatcacg aagaaagatg tcgccaagcg gactgcgaag
     2521 atcgtaggag aaaaaatata tctgaccgaa aaagtggtcg acggcgtttt tcaggccctg
     2581 cgggaattca tgtccgaggc caatcctgaa gtgcgcatcg agattcgtga cttcggcgtc
     2641 ttcgaagtga aaaagaccaa gcccaaaccc aaggcccgca accccaaaac cggtgaaatc
     2701 atctatgttc ccgctcgccg caagacccat ttcaaggcgg gcaaactgct caaagaggtc
     2761 ctgaaacagc cgctgcccct ggagagcaaa taacccaatc tcgcccgcgc gagatcgcag
     2821 tccggcatgg cagatctaac cccgattccc gttgagcagc tgcctcaggg acgcattgtc
     2881 gggatcgact atggcgagag gcgcattggc ctggcggtca gcgatccgtt gcaaaccata
     2941 gcctttcccc tggtcacttt gttccgcaaa gggcaggctt ttcccgttga acaaatcatc
     3001 gcttccgtgc aggagcaggc ggctgtggcg ctggtcgtaa gcctgcccct gcacatggat
     3061 ggcagcctgg gcgacaaggc gcaagccgca gaggcatt
//
LOCUS       DAOPWU010000386         3096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_32394_length_3096_cov_7.145676, whole genome shotgun sequence.
ACCESSION   DAOPWU010000386 DAOPWU010000000
VERSION     DAOPWU010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32394_length_3096_cov_7.145676"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKM23_14035"
     CDS             complement(<1..502)
                     /locus_tag="PKM23_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92639.1"
                     /translation="MADNCRWYSIGDITLQVESDLAFSEQTFSDKLELFRIASPGPDV
                     VQIRHHFGLPDMTGWDFSREKYRREPWAIYQTDAGWIYLGILPQGFEEKLWKVVVFNP
                     DHAQADIWHPDESAFLQGGATSLTLFPSDQILVARLLAGRSGCYLHSAGAIINGAGML
                     FVGHSEA"
     gene            complement(502..2772)
                     /locus_tag="PKM23_14040"
     CDS             complement(502..2772)
                     /locus_tag="PKM23_14040"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNY92640.1"
                     /translation="MPPEENAPAREWEFRDYLDLALRRKTVIIATFILVFLATAVYQL
                     TRTPVYSAYTSFIIDESATNTGVGSERYNPYAYWMREGKPIEYYQAIMGSQVYMDKML
                     VRLEADDDLKYAPGVTAEEINDAAGGISLSMDENSRLMTIASRAYSPIVAFKVAHIAT
                     EVFRERNQEIDVESAQDVVRFIDKQRQEAQDRLEEAERSLQQYSGAGQVVFIGEDGGM
                     MSKVSQVESILEQAETERRLAESNLASFNMQLEGAGKQGTGLQSAESFPEVAALRQRL
                     SNFEDQRNALAVSAGADSLNAAELDRKIESIKGELRRAILAHSTDSMPAEDRRTDELR
                     TMLTNRLVAEQINLSSSRNRERFYNNLLEEYRRQSPERLEKTMELARLRRTQAVHQNL
                     LNYLVERHEEAKIKAATGAGGIRIVNPASIPEKPNSRNIPRSLLIGAMLGLGLGFGLA
                     MAQEYLDQTVRSRQDLERLTGIQVIGQIPMGFKLNGKNGQKLPRPNIITSFLKTRLKK
                     GKASDAAEQQYYPLINKYDPHAPFAEAFRSLRTDLQFYSVDKPIKKLLITSSIPGEGK
                     TLVTANLAMAYAELGHRVVIVDADIRKPKQPTVFGVERKPGFTDYFMDLVPYDKIIRH
                     LPVRDLDLIPSGTVPPNPGEVLNSQKMADLIERLEKEYDYVLVDAPPVLSISDAKALS
                     RIIENMLLVFRFARTEKHYVREAVANLLNIKASIIGFVFNGIEVGNGKGYYKYYYYSE
                     YREKFDAPRVDKVQVN"
BASE COUNT          621 a          899 c          929 g          647 t
ORIGIN      
        1 ccgcctcgga gtggccgaca aagagcatcc ccgccccgtt gatgatcgcc ccggccgaat
       61 gcagataaca gccgctgcgg ccggccagca gccgcgccac caggatctga tccgagggaa
      121 acagggtcag cgaagtcgcg ccgccctgaa ggaaagccga ttcatcggga tgccagatat
      181 ccgcttgcgc atggtccggg ttgaaaacta ccaccttcca caacttttcc tcgaatccct
      241 gcggcagaat gccgagatag atccacccgg catcggtctg atagatcgcc cagggctcgc
      301 ggcggtactt ttcgcggctg aaatcccagc cggtcatatc cggcaggccg aaatgatgcc
      361 ggatctgcac cacatccggg cccggggagg ctatgcgaaa gagctcaagc ttgtcgctga
      421 aagtctgctc tgaaaaagcc aggtcgctct cgacctgcag cgtaatatct ccgatcgaat
      481 accagcggca gttatccgcc atcagttcac ctgcaccttg tcgacgcggg gcgcatcgaa
      541 cttttcccgg tactcggaat agtaatagta cttgtaatag cccttgccgt tgccgacctc
      601 gatgccgtta aagacaaagc cgatgatcga ggccttgatg ttgagcagat tggccaccgc
      661 ctcgcgcaca tagtgctttt cggtccgggc gaagcggaag accagcagca tgttctcgat
      721 gatccgcgac agcgccttgg cgtcgctgat gctcagcacc ggcggggcgt ccaccagcac
      781 ataatcgtac tctttttcca gccgttcgat caggtcggcc attttctggc tgttgagcac
      841 ttcgccggga ttgggcggca ctgtgccgga gggaatcaga tcgagatcgc gcaccggcag
      901 atggcggatg atcttgtcat agggcaccag gtccatgaaa tagtcggtaa agccgggctt
      961 gcgctccacg ccaaagaccg tcggctgctt gggtttgcgg atgtccgcgt ccacgatcac
     1021 caccctgtgg cccagttccg cataggccat ggccaggttg gcggtcacca gggtcttgcc
     1081 ttcgcccggg atcgagctgg tgatgagcag ctttttgatc ggcttgtcga cgctgtaaaa
     1141 ctgcaggtcg gtgcgcagcg agcggaaagc ctcggcgaag ggggcatggg gatcgtattt
     1201 gttgatcagc ggatagtact gctgctcggc ggcatccgac gccttcccct tcttcagccg
     1261 ggttttcagg aatgacgtga tgatgttcgg cctgggcagc ttttggccgt ttttcccgtt
     1321 cagtttaaag cccatcggga tctgcccgat cacctggatg ccggtgagcc gctcgaggtc
     1381 ctgccggctg cgcacggtct ggtcgagata ctcctgggcc atggccaggc cgaagcccag
     1441 gccgaggccg agcatggcgc cgatcagcag cgagcgcggg atattgcgcg aattcggttt
     1501 ttcagggatg gaggccggat tgacgatgcg gatgccgccc gcgccggtgg ccgccttgat
     1561 cttggcctct tcgtggcgct cgaccagata gttgagcagg ttctgatgca ccgcctgggt
     1621 gcggcgcagt cgcgccagtt ccatggtctt ttcgagccgc tccgggctct ggcggcggta
     1681 ctcttcgagc agattgttgt aaaatctctc ccggttgcgg ctggagctga ggttgatctg
     1741 ctcggcgacc agccggttgg tcagcatggt gcgcagctcg tcggtcctgc gatcttcggc
     1801 cggcatcgaa tcggtagagt gcgcgaggat ggcgcggcgc agttcgccct tgatcgactc
     1861 gatcttgcgg tccagttccg ccgcattcag gctgtcagcc ccggcggaaa cagccagggc
     1921 attgcgctgg tcctcaaagt tcgagagccg ctggcgcagg gcggccacct ccggaaagga
     1981 ttcggcggac tgcagcccgg ttccctgttt gcccgcgcct tccagctgca tattgaagga
     2041 ggcgagattg ctctcggcga ggcggcgctc ggtttcggcc tgttccagga tggactccac
     2101 ctgagagacc ttgctcatca tgccgccgtc ttcgccgatg aaaaccacct ggccggcgcc
     2161 gctgtactgc tgcagcgacc gttccgcctc ctcgaggcgg tcctgggcct cctggcgctg
     2221 cttgtcgata aagcgcacca catcctgggc gctctcgaca tcgatctcct gattgcgctc
     2281 acgaaagacc tcggtggcga tatgggccac tttaaaggcc acaatcggag agtaggcccg
     2341 ggatgcaatc gtcatcagcc gcgaattttc gtccattgag agcgagatcc cgccggccgc
     2401 gtcattgatc tcctccgcgg tcacacccgg agcgtatttc agatcatcat cggcctcaag
     2461 cctgacgagc attttatcca tatagacctg gctgcccatg atcgcctgat aatactcgat
     2521 cggcttgccc tcccgcatcc agtaggcgta gggattatag cgctcggaac cgacgccggt
     2581 attggtggcg ctttcatcga tgataaaaga ggtataggcg ctgtagaccg gcgtgcgggt
     2641 cagttgatag accgccgtag ccagaaagac cagtatgaac gtggcaatga tgaccgtttt
     2701 ccgccgcaga gcaaggtcga gatagtcgcg aaactcccac tctcttgccg gagcattctc
     2761 ttcaggaggc atggcttgtc cttagaaaca cttgtctgta atgcatgaaa tgatcgaatg
     2821 gtggcacaga acaacaatgc gccggaaggg tgggagattg ctcacagaac gcggagagag
     2881 gagaagcagc tgtccagaaa gtgactgatg agcaatgcga taaagcagac agaggatgca
     2941 gagacagagg atgcagagat gagggagaga aaaccgccac agagccccga tgccccccat
     3001 tagcctgatt aactatgacc ccacccgata gtttttgcgc ttataaatta agtgataaaa
     3061 atcaataatg caacactttt attccagctg taacga
//
LOCUS       DAOPWU010000387         3095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_32414_length_3095_cov_4.784539, whole genome shotgun sequence.
ACCESSION   DAOPWU010000387 DAOPWU010000000
VERSION     DAOPWU010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32414_length_3095_cov_4.784539"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKM23_14045"
     CDS             <1..193
                     /locus_tag="PKM23_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92641.1"
                     /translation="ALMRCGLPDTFLSQGTRDFMLHQAGLSTQAILATIQSVRAGLEN
                     GRSGRIRNLFRFNRHVKTT"
     gene            190..1086
                     /locus_tag="PKM23_14050"
     CDS             190..1086
                     /locus_tag="PKM23_14050"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNY92642.1"
                     /translation="MIIGIAGNKNKPRVRETILHFCARLDELGVKQLFDRELKEFLGL
                     GPECRCLPLVELGRQCDIMAAFGGDGTLLATASDIGASGTPILGVNLGGLGFLAEVPL
                     EEMDETIDDLVAGRFALIERMLLKVTIEQSGGRKSFHALNDVVIEKGSVPRLILVEAS
                     VDGTFLNTYRCDGVIIATPTGSTAYSLSAGGPLLMPTMEAIIVTPICPHSLTVRPIVL
                     SESCRLELGLVNHSHVAQVNIDGRNRLSLNPDDRVYVERGEYRIKWVDTGKRDFFNVL
                     RTKLNWGIDLTAIKSAGHPPEA"
     gene            1119..>3095
                     /gene="mutS"
                     /locus_tag="PKM23_14055"
     CDS             1119..>3095
                     /gene="mutS"
                     /locus_tag="PKM23_14055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNY92643.1"
                     /translation="MTKEQIKTPLMEQYMAIKSKHKDAVLFFRMGDFYEMFFDDARTG
                     AAVLGITLTSRAHGKSADVPLAGFPHHALDTYLAKMIKAGYRVAICEQTENPKFAKTI
                     VKREVLEVVTPGTTLVDDLLETKKNNYLLGLFVHDQRCGTAVVDVSTGEFRVGEIGFE
                     GLAEFILTLAPAEILLSAALQPAVEELLRGSPWITLTRREEWMFHHDYAVETLNDHFG
                     TLSLKGFGIEDLPLGISAAGGVLAYLRENQKEKLIHINRLARAADEERMIIDSSSRRN
                     LELVLSLHSARKHGTLLGTIDQTRTPMGGRMLVQWISYPLLNLAAIGERHDAVEELSR
                     DHAGRSAMIETLKQSGDLERLLARFATGRANARDAIAVLHSLRTVEELTLHLQPMSAS
                     MLAGACKQLNPLPELHDEIERALVQDPPLSVTDGGFIRSGYNSRLDELREIAFSGKDW
                     IARLQQQERERTGISSLKVNYTKVFGYFIEVTNPNLSRVPPYYIRKQTLVNAERFITP
                     ELKEYEEKVLGAEEKIVDLEYELFDQLRRRILADTIALQENARIIAQMDCLSCLAQVA
                     VDYGYVRPVMNEGDGLIIRDGRHPVIERLLPAGESFVVNDTRMETTREQLLIITGPNM
                     AGKSTYLRQVGLIAIMAQMGSFVPAASAEIGLVDR"
BASE COUNT          614 a         1026 c          897 g          558 t
ORIGIN      
        1 agcactgatg cgctgtggcc tgcccgacac ctttctcagc cagggcaccc gcgatttcat
       61 gctccatcag gcgggcctga gcacacaggc gatcctcgcc accatccaat ccgtacgggc
      121 cggcctcgag aacggccgct ccggcagaat tcgcaatctg ttccgcttca acaggcatgt
      181 gaaaaccaca tgatcatcgg gatcgccggc aacaagaaca aaccccgcgt ccgcgagacc
      241 atcctccact tctgcgcccg cctggatgaa ctcggggtta agcagctttt cgaccgggaa
      301 ctgaaggagt tcctcgggct gggacctgag tgccgctgcc tgcctctggt ggaactcggc
      361 cgccagtgcg acatcatggc cgccttcggg ggggacggca ccctgctcgc caccgcaagc
      421 gacatcggcg ccagcggaac ccccatcctc ggcgtcaatc tcgggggact gggctttctc
      481 gccgaggtgc ccctggagga gatggatgaa accatcgacg acctggtggc cggacgcttt
      541 gccctcatcg agcgcatgct cctcaaggtg actatcgagc agagcggcgg gcgtaaaagc
      601 tttcatgccc tcaacgatgt cgtcatcgaa aaggggagcg tgccccggct catcctggtc
      661 gaggccagcg tcgatggcac ctttctcaac acctaccgct gcgacggggt gatcatcgcc
      721 acaccgaccg gttcgacggc ctattccctt tcggccggcg ggcccctgct catgccgacc
      781 atggaggcga tcatcgtcac cccgatttgt ccccactccc tgactgtccg ccccatcgtc
      841 ctctcggaat catgccggct cgagctcggc ctggtcaacc attcccatgt cgcccaggtc
      901 aacatcgacg ggcgcaaccg cctcagcctc aatcccgacg accgggtcta cgtcgagcgc
      961 ggggagtacc ggatcaaatg ggtggatacc ggcaagcgcg actttttcaa cgtcttgcgc
     1021 accaagctca actgggggat cgatctgacc gcgatcaagt ccgccggaca ccctccagaa
     1081 gcctgagcgc aaaagagcac tttacgaaga gagcgacatt gaccaaagaa cagatcaaaa
     1141 cccccctgat ggagcagtac atggccatca agagcaagca caaggatgcc gtgctctttt
     1201 ttcgtatggg ggatttttac gagatgtttt ttgacgatgc caggaccggc gccgccgtgc
     1261 tcggcatcac cctcacttcg cgcgcgcacg gcaagtccgc cgacgtgcct ctcgccggct
     1321 ttccccatca cgccctcgac acctatctgg ctaaaatgat caaggcgggc tatcgcgtcg
     1381 ccatctgcga gcagaccgaa aacccaaagt tcgccaagac catcgtcaaa cgcgaggtgc
     1441 tcgaggtggt gacgccgggc accaccctcg tcgacgacct gctcgaaacc aagaagaaca
     1501 actatctgct cgggctgttc gtgcatgacc agcgctgcgg cacggccgtg gtcgatgtct
     1561 ccacagggga attcagggtg ggtgaaatcg gcttcgaggg tctggctgag tttatcctca
     1621 ccctcgctcc ggccgagatc ctgctcagtg cagcgctcca gccggccgtg gaggagctgc
     1681 tcaggggtag tccctggatc acactcaccc ggcgcgagga gtggatgttc catcatgatt
     1741 atgcggtcga gaccctcaac gaccacttcg gcaccctttc cctgaagggc ttcggcatcg
     1801 aggacttgcc gctcgggatc tcggccgccg gcggcgttct cgcctatctg cgcgagaacc
     1861 aaaaggagaa gctgatccat atcaaccggc tcgcgcgcgc cgccgatgag gaacgcatga
     1921 tcatcgacag cagttcgcgg cgcaatctcg agctggtgct ctccctccat tcggcgcgca
     1981 agcacgggac cctgctcggg acgatcgacc agaccaggac ccccatgggc gggcgcatgc
     2041 tcgtgcagtg gatcagctac cccctcctga acctcgccgc gatcggcgag cgccatgatg
     2101 ccgtggagga gttgagccgg gatcatgcgg ggcgcagcgc catgatcgaa accctcaagc
     2161 agagcggcga cctcgagcgc ctgctggccc gtttcgccac cggccgcgcc aatgcccggg
     2221 acgccattgc agtgctgcat tccctgcgta cggtcgagga actcaccctc catctgcagc
     2281 cgatgagtgc cagcatgctc gccggggcgt gcaagcagct caatcccctg cccgagctgc
     2341 acgacgagat cgagcgcgcc ctcgttcagg atccacccct gtcggtgacc gacggcggtt
     2401 ttatccgcag cggctataat tccagactcg atgaactgcg tgagatcgcc ttcagcggca
     2461 aggactggat cgcccgcctg cagcagcagg agagggagcg caccggcatc tcctcgctca
     2521 aggtcaatta caccaaggtc tttggctatt tcatcgaggt gaccaacccc aatctgtcgc
     2581 gggtgccgcc atattatatc cgcaagcaga ccctggtcaa cgccgagcgc tttatcactc
     2641 cggagctcaa ggagtacgag gagaaggtcc tcggcgccga ggaaaagatc gtcgatctgg
     2701 agtacgagct ttttgatcag ctgcgccggc gcatcctagc tgataccatc gcgctgcagg
     2761 aaaacgcccg catcatcgcc cagatggact gcctgagctg cctcgcacag gttgcggtcg
     2821 attatggcta tgtccgcccg gtcatgaatg aaggcgatgg gctgatcatc cgggatggac
     2881 gccacccggt tatcgagcgc ctgctcccgg cgggcgaatc cttcgtcgtc aacgacaccc
     2941 gcatggagac aaccagggag cagctgctga taatcaccgg acctaacatg gcagggaaat
     3001 cgacctatct gcgccaggtc ggactgatcg ccatcatggc ccagatgggc tctttcgtgc
     3061 cagccgcctc ggccgagatc gggctggtcg accgc
//
LOCUS       DAOPWU010000388         3095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_32418_length_3095_cov_3.618750, whole genome shotgun sequence.
ACCESSION   DAOPWU010000388 DAOPWU010000000
VERSION     DAOPWU010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32418_length_3095_cov_3.618750"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PKM23_14060"
     CDS             <1..284
                     /locus_tag="PKM23_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNY92644.1"
                     /translation="SSKVTLDKVAKTMIENPELEVEIQGHTDNVGKQASNIKLSQARA
                     ESVAKYLVGKGISASRMTTKGIGPDRPVASNDTAEGRQLNRRITFTRTK"
     gene            complement(358..1029)
                     /locus_tag="PKM23_14065"
     CDS             complement(358..1029)
                     /locus_tag="PKM23_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92645.1"
                     /translation="MSWLDYAALNLGYREWGNPVAVLQALDKEFNLPFLCANVLYKDS
                     ATTPFKPYAIRELEATPGRSKMPFKKLTIAMVGLTDNLLAQLFVNRPGEPELLYRDPV
                     EAAGELMPELRGKADLVILLYYGKYQKLTALLEAVPGFDVAVFGGEHYLVASQKSPNN
                     PVRIVSTPSMGKYAGVLTLQLDKKRKIVSSSDRQVPLKEDMNEVARFNELAAEYEKEA
                     SLTKP"
     gene            complement(1407..>3095)
                     /locus_tag="PKM23_14070"
     CDS             complement(1407..>3095)
                     /locus_tag="PKM23_14070"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY92646.1"
                     /translation="GAGEITGLRYNPAGTGLIEKSQLAFNVHNWIDDTKQGGIAAALP
                     TKIGVFSADFSYFDEGEIVELDENFQQLGGSSSSNDLLLTLGYANALRIGSNRLALGA
                     AFKLLHQDLIGERSTAYGADAGLLFHASWLGLSAGVQNIGITKISFNEQSNSLPLTYR
                     GGAALFLPLSNDFDLNLAADAALPADEEMRYYLGGEFIISDLIALRGGYKLHDVEASR
                     WAAGVGLNMPAEWLGRSRIRFDYAYAPIDEFDEASHRFSVLVRFNETGERMNAMGALD
                     RSALQEELDAAERSRRAAREAEERARAMEDRARKLEEELAERLARVKQIAAESEGKIE
                     VEPKSREKILVSMRINFDFDQARIRPDAFSTLHQVADILNTYPEARVQLSGHTDYIGT
                     DEYNIRLSQRRIDSVMVFLINKEKVGRGRFFMPVGYGESRPIATNETPEGRFRNRRVE
                     FLLYTMDVVPEMPEGTAIKAVEVIDDRTVRIICNGKMGFTTDMLDTPDRLVVDFPKGF
                     LLNDIASYELNRGPFLRARLGYHGEGFSRVVFDLKNAIDAEVTAQDNYIIITVK"
BASE COUNT          672 a          862 c          916 g          645 t
ORIGIN      
        1 cctcctccaa ggtgactttg gacaaggtcg cgaagaccat gatagaaaat cccgagctcg
       61 aggttgagat ccagggccat accgacaacg tcggcaagca ggcctcgaac ataaagctct
      121 cccaggctcg cgctgaatcg gtggccaagt atctggtggg caagggtatc tccgcgtccc
      181 gcatgaccac caagggcatc ggccccgatc gtccggttgc cagcaacgac accgctgaag
      241 ggcgtcagct caaccgccgt ataaccttca ccagaaccaa ataagcttcc ttttacaaaa
      301 aaagcgtcca tctctgagag atggacgctt ttttttgcct ctaaatcatt ccgccgatta
      361 cggcttggtg aggctcgcct ccttttcgta ctctgcagcc aactcattga aacgggccac
      421 ttcgttcata tcctctttca agggcacctg acggtcgctc gaggagacga ttttgcgttt
      481 cttatccagc tgcagggtaa gaacgcctgc gtattttccc atcgatggag tgctgacgat
      541 gcgaacggga ttattggggc ttttctggct ggcgacgaga tagtgctctc cgccgaatac
      601 agcaacatcg aaacccggga cggcctcgag cagggcggtc aatttctggt acttgccgta
      661 gtagaggaga atgaccagat cggccttccc cctgagctct ggcataagct cccccgctgc
      721 ctcgaccgga tcccggtaga gcagctccgg ctctcccggc cggttgacaa agagctgggc
      781 gaggagattg tccgtcaaac ccaccatggc gatggtaagt tttttaaagg gcatcttgct
      841 gcggccgggc gtggcttcca gttccctgat agcatacggt ttgaaaggag tggttgccga
      901 atccttgtaa aggacgttgg cgcagagaaa gggcagattg aactccttgt caagcgcctg
      961 cagcactgca acgggattgc cccattcgcg atagccaaga ttgagcgccg cataatcgag
     1021 ccagctcatg cccttgacga ggtagcggtt ctgcagctgg ctgaaggtgt tattgccgcg
     1081 tgcgaagcca ccggcatcga caagcagggt aaaatcagcc gtggaacgat cattttcgag
     1141 aaaaccggcc cgccgggcca gtccgccgta gcgtgcagac tttcagccac agggggtcag
     1201 gtagccgatc aaatcattcg aataatagat attcaggctc gctcctgccc cctcctgcgc
     1261 aaacgtgcag gttgaaaagg ccagaagcgc ggcaaaaagc gtagtagcat tgcgaaaaaa
     1321 gtgcatttta cgattccatt ctgattgcaa gcgtgtgctc tcaggttggg gcgacggatg
     1381 atccgcctcc ccagccctga aggaagttat ttaacggtga taatgatata gttgtcctgc
     1441 gcagtcacct ccgcatcgat cgcattttta agatcaaaga cgacgcggga gaaaccttcg
     1501 ccatgatagc caaggcgggc gcgcaggaag gggccacggt taagctcata gctggcgata
     1561 tcattgagca aaaagccctt gggaaaatca accacaaggc ggtccggagt atcgagcata
     1621 tcggtggtga aacccatctt gccattgcag atgatgcgca cagtgcggtc atcgatgacc
     1681 tcaacggcct tgatggcggt cccttccggc atttccggca caacatccat ggtgtagaga
     1741 aggaattcga cgcggcggtt gcggaagcgt ccttccgggg tttcgttggt ggcgatcggc
     1801 cggctttcgc catagcccac gggcatgaag aagcgtccgc gtccgacctt ctccttgttg
     1861 atcaggaaga ccatgacgct gtcgatgcgt cgctgcgaga ggcgaatatt gtattcatca
     1921 gtgccgatgt agtcggtatg gccggagagc tgaaccctgg cctcgggata ggtgttgagg
     1981 atatcggcca cctggtgaag agtgctaaag gcgtcggggc ggattcgcgc ctgatcgaaa
     2041 tcgaagttga tgcgcatgct gacgaggatc ttctcgcggg atttgggttc gacctcgatc
     2101 ttgccttccg attcggcagc gatctgcttg acccgggcaa gccgttccgc aagctcctcc
     2161 tccagctttc tggcgcgatc ctccatggca cgggcgcgct cctccgcttc cctggcggcg
     2221 cggcgggatc tctccgcagc atcaagctcc tcctgcagag cgctgcggtc cagggcgccc
     2281 atggcattca tcctctctcc ggtctcgttg aagcgcacca ggacggagaa gcggtgcgag
     2341 gcctcgtcga actcgtcgat gggggcatag gcataatcga agcggatacg ggatcggccc
     2401 agccattcag cgggcatgtt gaggccaacg ccggcggccc agcgcgaagc ctcgacatca
     2461 tgcagcttgt atcccccgcg cagggcaatc agatcactaa taatgaactc acctccgaga
     2521 taatagcgca tctcctcatc ggcaggaagg gctgcatctg cagcgagatt gagatcaaaa
     2581 tcattcgaga gaggcaaaaa gagggcggca ccgccgcgat aggtcaatgg cagcgagttc
     2641 gactgttcgt tgaaggagat cttggtgatg ccgatattct gcaccccggc ggacaggccc
     2701 aaccagctgg cgtggaagag caggccggca tcggctccgt aggcggtgct gcgctcaccg
     2761 atgaggtcct ggtgaagcag cttgaaggcg gcgccgaggg cgaggcggtt tgatccgatc
     2821 ctgagagcgt tggcgtagcc gagggtgaga aggagatcat tactgctgct gctcccgccc
     2881 agctgctgga aattctcgtc cagttcaacg atttcgccct cgtcgaaata gctgaaatcg
     2941 gccgaaaaga cgccgatttt ggtgggaagg gcggcggcga tgccgccctg tttggtatca
     3001 tcgatccagt tgtggacatt aaaagcgagc tggctcttct cgatcagacc ggttcccgcc
     3061 ggattgtagc gcaggccggt gatctcgcca gcgcc
//
LOCUS       DAOPWU010000389         3089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_32500_length_3089_cov_3.710613, whole genome shotgun sequence.
ACCESSION   DAOPWU010000389 DAOPWU010000000
VERSION     DAOPWU010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32500_length_3089_cov_3.710613"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="PKM23_14075"
     CDS             complement(<1..2004)
                     /locus_tag="PKM23_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92647.1"
                     /translation="MRKRRWVLHILLLILALTILLPRAWRWTRMDDKAKQFLLARMQP
                     VLGDAFAIDRVSISFANIHLLGLYLPPGEQPFSVRIEDLRIGINPIRMFRYGMDPRAF
                     SQDIMIKKPVVLIRTAPGALTRDSWPQGEKIRLYLHNLQRLDFLKMASIQEGAVALER
                     DGREPFPLITGLKGWLINENPDTLLLRLSGHPLGARQSLMTLEGRFGLEEGGLDTIQT
                     TFSRLPLSRLLTPGEDREWLIHSGEAEADISLALRPDREYQPNLSGWIRVANLAASWP
                     ERHLRCDHSGITLRLQEGRVEVERSALWINQTSMSLTGAITGPANPHLQLTLSAPEID
                     AGSFRDTLPEELRDLTGQVALNVDLEGPLASPQLRFGLLSTALHLRDELFEEVSLNAN
                     WTGDSLQVQSLRGRWRGLDIEAVGALSGIGDSTSLEGRLNAAGELLPLLPPAYTGSLR
                     QARLSLAAALSGPAAAPDAQGSLSLSLANHTGGAMTSESRFLLRDRRFQINPAGGPDE
                     GIRGVIDWSGEEPRFALNLPDARSWLEMLWPAPAGAMRQRGLDFSLHLNHLGGHYALQ
                     ARLLHSRPGRPVEELFALSSRLERLDGGWQGIGTTTLWPDQTRAVRGHFSLAADSMRV
                     SLRDWTMGEWFSGILQIGRSGSLSGSCFLQDFPVELLGKGGAET"
     gene            complement(2094..2837)
                     /locus_tag="PKM23_14080"
     CDS             complement(2094..2837)
                     /locus_tag="PKM23_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNY92648.1"
                     /translation="MIDQERVNRIKARGNVPRHVAVIMDGNGRWAKGRGLPRTEGHKE
                     GIESVRAAVEAAGALGIKALTLYTFSSENWARPRSEVAALMSLLVRTVRREAADLDRN
                     NVRLTVIGDLEQLPPTPRMGFKATIARLQKNTGLILNLALSYSGRQEIVLAARNIAQE
                     AAAGRLDPDTIDETCFAGHLQTAPIGDPDLLIRTSGEMRLSNFLLWQLAYSELYVTPI
                     LWPDFRHDQFLDAIENYQMRDRRFGKVTG"
BASE COUNT          555 a          879 c         1126 g          529 t
ORIGIN      
        1 ggtttcggcg ccgcctttgc cgagcagctc gaccggaaag tcctgaagaa agcatgagcc
       61 ggagaggctt ccgctgcggc cgatctgaag gatgccggaa aaccattcgc ccatggtcca
      121 atcgcggaga gagactcgca tgctgtctgc ggcgaggctg aagtggccgc gtaccgccct
      181 ggtctgatcg ggccaaaggg tggtggtgcc gatcccctgc cagccgccgt ccaggcgctc
      241 gaggcgggag gagagtgcga agagctcttc caccggccgg ccgggacggc tgtgcagcag
      301 ccgcgcctga agggcgtaat ggccgccgag atggttgagg tggagggaaa aatcgaggcc
      361 gcgctggcgc atggcgccgg cgggcgcggg ccagagcatt tccagccagg agcgggcgtc
      421 gggcaggtta agggcaaagc ggggctcctc cccgctccag tcgatgacgc ccctgatccc
      481 ctcatcgggt ccgccggcgg gattgatctg gaagcggcgg tcgcggagga ggaagcggga
      541 ttcagaggtc atggcccctc ccgtgtggtt ggcgaggctg agggagaggc tcccctgcgc
      601 gtccggagcg gcggccgggc ctgagagggc ggccgcaagc gagagccggg cctggcgcag
      661 gctgcctgta taggcgggag gcagaagagg cagaagctcg cccgcggcgt tgaggcggcc
      721 ctcgaggctg gtcgagtcgc cgattccgct cagggcgccg acggcctcga tgtcgagtcc
      781 gcgccagcgc ccgcgcaggg actgcacctg caggctgtcg cccgtccagt ttgcgttcag
      841 gctgacctcc tcgaagagtt catctcgcag gtgcagggcg gtcgagagca gcccgaaacg
      901 cagctgcggc gaagcaagtg gaccttcgag gtcgacgttg agggcgactt gcccggtcag
      961 atcgcgcagc tcttccggca gtgtgtcacg aaagctcccg gcatcgatct ccggcgccga
     1021 cagggtgagc tggaggtgtg gattggccgg gccggtgatg gcgccggtca ggctcatgga
     1081 ggtctggttg atccagagcg ccgagcgctc cacctccacc ctcccctcct gcaggcgcag
     1141 ggtgatgccc gaatgatcgc agcgcagatg gcgttccggc caggaggcgg ccaggttggc
     1201 gacccggatc cagccggaga gattcggctg gtactcccgg tcagggcgca gggcgagcga
     1261 gatgtcggcc tcggcctcgc cgctgtggat cagccattcg cggtcttcgc cgggggtcag
     1321 gagccgcgag agggggaggc gcgagaaggt ggtctggatg gtgtccagcc ccccctcttc
     1381 gaggccgaag cggccctcga gggtcatcaa gctctggcgg gcgccgaggg gatggccgct
     1441 gaggcgcagc agcagcgtat cgggattttc attgataagc cagcccttga ggccggtgat
     1501 gaggggaaag ggctcccggc cgtcgcgttc gagcgcgacc gcgccctcct ggatggaagc
     1561 catcttgaga aaatcgagcc tttgcagatt gtggaggtag aggcggatct tttcaccctg
     1621 gggccaggag tcgcgggtca gcgcgccggg cgcggtccgg atgaggacga ccggcttctt
     1681 gatcatgata tcctgggaga aggcccgggg gtccatgcca tagcggaaca tgcgaatggg
     1741 attgatgccg atgcgcaggt cctcgatgcg gaccgagaag ggttgttcgc cgggcgggag
     1801 atagaggccg agaagatgga tattggcgaa gctgatgctc acccggtcga tggcaaaggc
     1861 gtcgccgagg acgggctgca ttctggcgag caggaactgc ttggccttgt cgtccattct
     1921 ggtccagcgc caggcgcggg gcagcaggat ggtgagggcg aggatgagga ggagtatgtg
     1981 cagtacccag cgtcgcttgc gcacggattg tatgctttca acgggttagc gtgaagagag
     2041 ccatcgggaa tcacattggg gagcagggcg cgcgggcagg tgaagcggtc ggattatccg
     2101 gtgaccttgc cgaagcgccg atcgcgcatc tgatagttct cgatggcgtc caggaactga
     2161 tcatggcgaa aatccggcca caggatcggg gtgacgtaca actcggaata ggccagctgc
     2221 cagagcaaaa agttgcttag gcgcatctcg ccgctggtgc ggatgaggag atcgggatcg
     2281 ccgatggggg cggtctgaag atggccggcg aagcaggttt catcgatggt gtcggggtcg
     2341 agccgtccgg cggcggcctc ctgtgcgata ttccgggcgg cgaggacgat ctcctgccgg
     2401 ccgctgtaac tgagggcgag gttgaggatg aggccggtgt tcttctgcag gcgggcaatg
     2461 gtcgccttga aacccatacg cggggtgggc ggcagctgtt cgaggtcgcc gataacagtg
     2521 aggcggacat tgttgcggtc gaggtcggcg gcctcgcggc ggacggtccg gaccagcaga
     2581 gacatcagcg cggccacctc gctgcgcggc ctcgcccagt tctccgaaga aaaggtatag
     2641 agggtgaggg ccttgatccc gagcgccccg gccgcctcga cggcggcgcg caccgattcg
     2701 atcccctcct tgtggccttc cgtgcggggc agtcccctgc ctttggccca gcggccattg
     2761 ccatccatga ttaccgcgac atgccggggc acattgccgc gcgccttgat gcggttgact
     2821 cgttcctgat cgatcacaat tcgctcatca ttcgtctgaa tacctgggag agaagggcca
     2881 tgcggccatt tttgcaggtg cagccgcaag tgaacataat aaacacaata ttcagaacat
     2941 attcaaggta aaaaaaagcc ccacagacag gtgtggggct ccgctcccgc aaaagggaaa
     3001 actatttgcg catctcttca gccttgttga agaattccgc gcccttggtc gcttcgccga
     3061 cgttgacata agcgacgccg aggttgtaa
//
LOCUS       DAOPWU010000390         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_32511_length_3088_cov_4.460930, whole genome shotgun sequence.
ACCESSION   DAOPWU010000390 DAOPWU010000000
VERSION     DAOPWU010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32511_length_3088_cov_4.460930"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PKM23_14085"
     CDS             <1..265
                     /locus_tag="PKM23_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92649.1"
                     /translation="EGYSRRISWRLGGYYSRLPFTNADGENVHETFLSFGCGLPFSVT
                     AGQVDIAFEVGRRGDVSRFMYEDTIFRLSGSLTGGERWFQRRY"
     gene            290..733
                     /gene="rpiB"
                     /locus_tag="PKM23_14090"
     CDS             290..733
                     /gene="rpiB"
                     /locus_tag="PKM23_14090"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNY92650.1"
                     /translation="MAIALGSDHAGYALKQALRRWLDEQSIEYTDFGCFEESPSVDYA
                     DFSFRVASAVSSGVCSRGVVICGTGIGAMITANKVGNIRAALCCSDYMAEMARRHNDA
                     NIIAFGGRTTTPELAVSMLRIFLDTPFEGGRHFGRVEKIHHLTGR"
     gene            758..2050
                     /gene="glyA"
                     /locus_tag="PKM23_14095"
     CDS             758..2050
                     /gene="glyA"
                     /locus_tag="PKM23_14095"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNY92651.1"
                     /translation="MSIIRTQDPAVYAAIQKEIHRQNDKLELIASENFVSPAVLEAVG
                     SPLTNKYAEGYPGKRYYGGCEFVDEAENLARDRAKQLFGADHANVQPHSGSQANLAAF
                     FSLVNYGDKVMGMDLAHGGHLTHGSPVNFSGRFFRMVHYGVGRDGFLDMDEIESLARK
                     EKPAMIIAGASAYSRAIDYAAFRRIADAVGAKLVADIAHPAGVIAAGLIPSPVPHCHL
                     TTSTTHKTLRGPRGGLILVGVDGENDLGITTPKGQLKKWSEIVDSQIFPGFQGGPLMH
                     TIAAKAVAFGEALQPAFKSYQEQVVRNARTLAAALIELGHKIVSGGTDTHLLLIDLSD
                     RGLTGKEVERVLDEAGITVNKNMVPFDRQSPFQTSGIRIGTAALTTRGMKEEEMREVA
                     AMIDTVILHLHDEKKLDRVRAGVRELTGRYPLYEALRV"
     gene            2247..2693
                     /gene="nrdR"
                     /locus_tag="PKM23_14100"
     CDS             2247..2693
                     /gene="nrdR"
                     /locus_tag="PKM23_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNY92652.1"
                     /translation="MRCPFCSYHDSKVIDSRTRDGGRTIRRRRECLKCQRRFTTREQL
                     DEIPLWVIKTDNRREEFDRQKLVRSLQVACIKRPISMATIEQITSKIEYDLRDRGVEE
                     VESTEIGEAVMMALKELDEIAYLRFASVYRNFQVKEEFIQQLNLLK"
     gene            2808..>3088
                     /locus_tag="PKM23_14105"
     CDS             2808..>3088
                     /locus_tag="PKM23_14105"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNY92653.1"
                     /translation="MERNLGLFKRGSFKGGVHPAENKRWTEAKAVEKLPIPPEVIIPV
                     QQHIGAPSRPVVAVGDKVRAGDPISAASGFVSVPAHASISGTVAAIEERP"
BASE COUNT          598 a         1073 c          866 g          551 t
ORIGIN      
        1 ggagggctac agccgccgca tctcctggcg cctcggcggc tattacagcc ggcttccctt
       61 caccaatgcg gacggcgaga atgtgcacga gacctttctc tccttcggct gcggcctgcc
      121 cttttcggtg accgccggcc aggtcgacat cgccttcgag gtgggccggc gcggcgacgt
      181 cagccgcttc atgtacgagg ataccatctt ccggctgagc gggagtttga ccggcggcga
      241 gcgctggttc cagagacgct attaaccttt ctcgaacggg gtccccacca tggccattgc
      301 cctcggatca gaccatgccg gttacgccct caagcaggcg ctgcgccggt ggcttgacga
      361 gcagagcatc gaatacaccg atttcggctg ctttgaagag tccccctctg tggactatgc
      421 cgatttcagc ttccgggtcg cctcggcggt gagcagcggg gtctgcagcc gcggggtggt
      481 catctgcggc accggcatcg gggccatgat caccgccaac aaggtgggca atatccgcgc
      541 cgccctctgc tgcagcgact atatggccga gatggcgcgc cgccataacg atgccaacat
      601 catcgccttc ggcggccgca ccaccacccc ggaactggcc gtcagcatgc tgcgcatctt
      661 cctcgacacc ccctttgaag gcggccgcca tttcggccgc gttgaaaaaa tccatcatct
      721 caccggacgt tgatcccatc cgccacaagg agtgaccatg tcgatcatca gaacccagga
      781 tccggcggtc tatgcggcca tccagaaaga aatccaccgt cagaacgaca aactggaact
      841 gatcgcttca gagaatttcg tcagtcctgc tgtactggag gcggtgggat caccactgac
      901 caacaagtac gccgagggct atccgggcaa gcgctattac ggcggctgtg aattcgtcga
      961 cgaggccgag aatctcgccc gcgaccgcgc caagcagctc ttcggagcgg accacgccaa
     1021 cgtccagccc cactccggct cgcaggccaa cctcgccgcc tttttcagcc tggtcaatta
     1081 cggcgacaag gtgatgggta tggatctcgc ccacggcggc catctcaccc acggcagccc
     1141 ggtcaatttc tccggccgtt ttttcaggat ggtccattac ggagtcggcc gcgacggctt
     1201 tctcgacatg gacgagatcg aatccctggc ccgcaaggaa aaaccggcga tgatcatcgc
     1261 cggggcgagc gcctactccc gcgccatcga ctatgccgct ttccgccgca tcgccgatgc
     1321 ggtcggggcc aaactggtgg ccgatatcgc ccaccccgcc ggggtcatcg ccgccgggct
     1381 catccccagt cccgttcccc actgccacct caccaccagc accacccaca agaccctgcg
     1441 cggcccgcgc ggcggcctga tcctggtcgg cgtggacggc gagaacgatc tcggcatcac
     1501 cacccccaag ggccagctca aaaagtggtc cgagatcgtc gacagccaga tctttcccgg
     1561 ctttcagggc ggcccgctca tgcacaccat cgccgccaag gcggtggcct tcggcgaggc
     1621 cctccagccc gccttcaaaa gttaccagga gcaggtggtc cgcaacgccc gcaccctggc
     1681 cgccgccctc atcgagctcg gtcacaagat cgtctccggc ggcaccgaca ctcatctcct
     1741 gctcatcgac ctcagcgacc gcggcctcac cggcaaggag gttgagcgcg tcctcgacga
     1801 ggccggcatc accgtcaaca agaacatggt ccccttcgac cggcagagcc ccttccagac
     1861 cagcggtatc cgcatcggca ccgccgccct caccacccgc ggcatgaaag aggaggagat
     1921 gcgcgaggtc gcggccatga tcgatacggt cattctccac ctgcacgatg aaaagaagct
     1981 cgaccgggtc cgcgccggag tccgcgagct gaccggccgc taccccctct acgaggccct
     2041 gcgggtataa ggcattcctg ctgtgctccc gcccggaagg gcgggagcgc tccatcgccg
     2101 cgcccccgcc tctcctcgct gacccgttca ccagcgctcc cgcagcggct tttcccgcat
     2161 tccagaaatt ccttgaattc ctgcaaacag ttgcttatat tcttgccatt gcaagtcctg
     2221 atttatgacc tcaccgggat gtcggcatga gatgcccttt ctgcagctac catgacagca
     2281 aagtgatcga ctcgcgtacg cgcgacggcg gccgcactat ccgccgcagg cgggagtgtc
     2341 tcaaatgcca gcgccgtttc accacccgcg agcagctgga tgagatcccc ctctgggtga
     2401 tcaagaccga taaccggcgc gaggaattcg accggcaaaa gctcgtccgc agcctgcagg
     2461 tggcctgcat caaacgcccg atctccatgg ccaccatcga acagatcacc tccaaaatcg
     2521 aatatgacct gcgcgatcgc ggcgtcgaag aggtcgaatc gaccgagatc ggcgaagcgg
     2581 tgatgatggc cctcaaggag ctggacgaga tcgcctatct ccgttttgcc tcggtctaca
     2641 gaaattttca ggtcaaggaa gaattcatcc agcagctcaa tctgctgaaa tagatcccgg
     2701 gcgcccgcag cgctgttgtt ctggccacgg ccaactcctc tttgcaccct ccccggtgcg
     2761 aatggaactc tgcccgcgaa cgctgtttgc cggtactctt gccaaccatg gagcggaatt
     2821 tgggtctctt caaacgaggt tctttcaagg gaggtgttca tccggcggag aataaacgct
     2881 ggaccgaagc caaagcggta gaaaaactgc ccattccacc agaagtcatc atccccgtac
     2941 aacagcatat cggcgccccc tcacggcccg tggttgcggt cggtgacaag gtgcgcgccg
     3001 gcgatcccat cagcgcggcc tccggatttg tctccgtgcc ggcccatgcc agcatcagcg
     3061 gcaccgtggc cgccatcgag gagcggcc
//
LOCUS       DAOPWU010000391         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_32734_length_3073_cov_4.118953, whole genome shotgun sequence.
ACCESSION   DAOPWU010000391 DAOPWU010000000
VERSION     DAOPWU010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32734_length_3073_cov_4.118953"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PKM23_14110"
     CDS             complement(<1..408)
                     /locus_tag="PKM23_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92654.1"
                     /translation="MKTKLFTTVLAAAVLLLFTFAQAQTTTPPVQHGSNFVDENGDGY
                     NDNAPDADGDGIPNGQDPDYVKPADGSGAKQAKGQGKGQSPNQGQGARTGAKGFIDEN
                     GDGINDNAPDADGDGVPNGQDPDYVKPGDGTGQS"
     gene            736..2154
                     /locus_tag="PKM23_14115"
     CDS             736..2154
                     /locus_tag="PKM23_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY92655.1"
                     /translation="MNPTKSAISRRSFLMGSAAAATALTMAPGDLLADEKKKSPSDKL
                     NIACIGAGGKGSSDIVDAATDNIVAICDVDDEKFADLFNHCKENRPDALPMLEKAKRY
                     KDYRIMLEENARLIDAVTVSTPDHTHAPAAMMALKMKKHVWVQKPLTHTVHEARMLAE
                     EAARQGVTTQMGNQGHASEDARLMNEWIWDGAIGEVREVHVWTNRPIWPQGVEAPATI
                     PSVPPSLDWNLWLGPAAWRPYHPDLCHFVWRGWLDFGTGAIGDMGAHILDHPYWALNL
                     GRPRFIHASSTEIKSTSYPLAEIITYYFPERSGHPEAESYGIKPKIGFPPVKMVWYDG
                     GLTPPRFMELEAGRKLGSGKGGAIFFGDKGIWMCGEYGASPRLIPETRMQEYKRPEKK
                     IPRVKSHMQDWIESCKAGKKSFTDFSYSGPLTETMLLGCVAVRCQEANMLLEYDAVNG
                     KFVNYPEADALLTKSYREGWTL"
     gene            2185..>3073
                     /locus_tag="PKM23_14120"
     CDS             2185..>3073
                     /locus_tag="PKM23_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY92656.1"
                     /translation="MNEEEKSKSPGRAIDRRDFLQGLAGVPVIGAFALAALKQESFRR
                     ASRARQAAATAGGEAPAELRIALLGVGAQGSVLLNSMLKIPGLRFQAVCDIWPEYNLK
                     RAGNLLKKYGHEAALYHDYREMLDKEKGLDAVVIATPDFWHADHTIACLQAGLHVYCE
                     KEMSNTLEGARRMVQAARESGKLLQIGHQRRSNPRYIFCRDTLLQELQLIGRPVTAFG
                     QWNRSAAPDLGWPKKYEIPADQLRRYGYDSMAQFCNWRWYKKLGGGPIVDLGSHQIDI
                     FSWFLGANPKSVQASGGTDY"
BASE COUNT          640 a          933 c          900 g          600 t
ORIGIN      
        1 gctctgtcct gtgccatcgc ccggcttgac atagtcggga tcctggccgt tggggacgcc
       61 gtcgccatca gcatcagggg cgttgtcatt gatgccgtcg ccgttttcat cgataaagcc
      121 cttggcgccg gtgcgggcgc cctggccctg attgggcgac tggcccttgc cctgaccctt
      181 ggcttgtttg gcaccgctgc cgtcggccgg tttgacatag tcgggatcct ggccattggg
      241 aatgccatcg ccgtcggcat ccggggcgtt gtcattgtag ccgtcgccgt tctcgtcgac
      301 gaaattgctg ccgtgctgaa cggggggcgt ggtggtctgt gcctgggcga aggtgaaaag
      361 cagcagcacg gccgctgcca gtacggtggt gaagagtttc gttttcatga tgacctccgt
      421 gatacggtaa tgagcgatta taatgaattg tgtcgaacgt tagttgttca tcacaccggg
      481 cttttgcaat tgctgtgcca aaaatgaagt aaaattgtaa aataaaataa aacaaatatt
      541 aaagatgcca taccagtacg acgcctgcgg gagagggcct gtcatctcga cgatttggtc
      601 gaaccccacg accatccggt cgttcttccc cggtgcaggt ctgtgcaaaa tcgcccatca
      661 tttcccttga ttatcaccca aaaaattgct aatcttgttg aaacgtttcc tttagcgtag
      721 gagagcatgg agcatatgaa tccgaccaag agcgctattt cgcgccgcag ttttctgatg
      781 ggaagcgcgg ccgccgccac cgccctgacc atggcccccg gggaccttct tgccgatgaa
      841 aagaaaaagt ctcccagcga caagctcaac atcgcctgca tcggcgccgg cggcaaaggg
      901 agttcggata tcgtcgatgc cgccaccgac aatattgtgg ccatttgcga cgtcgacgac
      961 gaaaaattcg ccgatctctt caaccattgc aaggagaacc gtccggatgc cctgccgatg
     1021 ctggaaaagg ccaaacgcta caaggactac cggatcatgc tcgaagaaaa cgccaggctc
     1081 atcgacgccg tgaccgtgag cacgccggat catacccatg ccccggcggc gatgatggcc
     1141 ctcaagatga agaagcatgt ctgggtgcaa aaaccgctca cccataccgt gcatgaggcg
     1201 agaatgctgg ccgaagaggc ggcgcggcag ggggtcacaa cccagatggg caaccagggt
     1261 cacgcctccg aggatgcccg gctgatgaac gaatggatct gggacggcgc catcggcgag
     1321 gtgcgcgagg tgcatgtctg gaccaaccgt ccgatctggc cgcagggtgt cgaggccccg
     1381 gcgaccatcc cctcggtccc gccctccctg gactggaacc tctggctggg tccggcggca
     1441 tggcgcccct accatcccga cctctgtcat ttcgtctggc gcggctggct cgatttcggt
     1501 accggtgcca tcggcgatat gggcgcccac atcctcgacc atccctactg ggcgctcaat
     1561 cttggccgtc cccgcttcat tcatgcctcc tcgaccgaaa tcaaatcgac ctcctatcct
     1621 ctcgccgaga tcatcaccta ttatttcccg gagcgttccg gacatcccga ggcggaaagc
     1681 tacggcatca agccaaaaat cggtttcccg ccggtcaaga tggtctggta tgatggcgga
     1741 ttgacaccgc cgcgcttcat ggagctggag gccgggcgca aactcggttc gggcaagggc
     1801 ggggcgatct ttttcgggga caagggcatt tggatgtgcg gggagtacgg cgccagcccg
     1861 cggcttatcc ccgagacccg catgcaggag tacaagcgcc cggagaaaaa gatcccgcgc
     1921 gtcaagagcc atatgcagga ctggattgag agctgcaagg ccggaaagaa atccttcacc
     1981 gatttcagct attccggccc cctcaccgag accatgctgc tcggctgcgt ggcggtgcgc
     2041 tgccaggagg ccaatatgct cctcgagtat gacgccgtca acggcaaatt cgtcaattat
     2101 cccgaagccg atgcccttct caccaagagc tatcgtgaag ggtggacgct ctaacctgac
     2161 cgcagtgacc gacggacgaa attcatgaac gaagaagaaa aaagcaaatc accaggccgt
     2221 gcgatcgacc ggcgggactt cctgcagggg ctggccgggg ttccggtcat cggggctttc
     2281 gccctggcgg cgctgaaaca ggagtccttc cgcagagcgt ccagagcgag gcaggcagcc
     2341 gctacggcag ggggagaagc gcccgccgaa ctccgcatcg ccctgctcgg cgtcggcgcc
     2401 cagggcagcg ttttgctcaa ttccatgctc aagatccccg gactgcgttt tcaggcggtt
     2461 tgcgatatct ggcccgaata caacctcaag cgcgccggca acttgctcaa aaaatatggc
     2521 catgaggcgg cgctctatca cgattatcgc gaaatgctcg acaaggagaa agggctcgat
     2581 gcggtggtca tcgccacgcc cgatttctgg cacgctgacc ataccatcgc ctgcctccag
     2641 gccggtctgc atgtctactg cgaaaaggag atgtccaaca ccctcgaggg ggcccggcgt
     2701 atggtgcagg ccgcccgcga gagcggcaag ctcctccaga tcggccatca gcgccgcagc
     2761 aatccccgct acatcttttg ccgtgataca ctgctgcaag aactgcaatt gatcggccgg
     2821 ccggtcaccg ccttcgggca gtggaaccgc tcggctgcgc cggatctggg ctggccgaag
     2881 aaatatgaga tccctgcaga ccagctccgc cgctacggct atgactcgat ggcgcagttc
     2941 tgcaactggc gctggtacaa aaagctgggc ggaggaccga tcgtcgatct cggctcccac
     3001 cagatcgata tcttttcctg gttcctcggc gccaatccca aatcggtcca ggccagcggt
     3061 ggcaccgatt act
//
LOCUS       DAOPWU010000392         3065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_32847_length_3065_cov_3.892359, whole genome shotgun sequence.
ACCESSION   DAOPWU010000392 DAOPWU010000000
VERSION     DAOPWU010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32847_length_3065_cov_3.892359"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKM23_14125"
     CDS             complement(<1..279)
                     /locus_tag="PKM23_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92657.1"
                     /translation="MTTHSVHLPRPIQLPGRRLLTSFHLRGWHLFVLLVMCEAILTWF
                     YQSQVMTRDLYHSLLKEQLEVQRIDAIFDLLKRISVWGYVTIPLFIGFR"
     gene            complement(344..2701)
                     /locus_tag="PKM23_14130"
     CDS             complement(344..2701)
                     /locus_tag="PKM23_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNY92658.1"
                     /translation="MRIRSRFPLVRQYDQLDCGPAALLSVLRFYRGDTTLVHARQLCR
                     TDIQGTTMLDMVRAARAMGFKAQGARGSFEQLAKEKLPCIAHVILPDGMSHFLVIYKI
                     EGERLLAGDPGKGRCKLERREFEKMWREHAVILLEPDGAPHTAAQPANTPNNALLHEE
                     SRSWLRWIGGYLRSQQSWLQQILFTGLLYTAAGLLTALFVQTLIDRLIPAKDLDNLLF
                     IASALLLLLALRAGIGYLRDRLLVTANKRLSLAVTGDFLLHLFRLPKSFFDTRRTGDI
                     TSRIDDSLRIHRTCLLLMQSLLLDLMLVAGSLLFMFWFSSLLGALTLAMLPFYTAVLW
                     RQASRLKKQQREVMKSHAGVEAAYIDSIQGMPEIMSFSAAPAFTRLNQSLFGLFQDHI
                     ARRGLMQAGLTLAVGLLGAFISVTLLALGARQVIAGELLLGQFMAAYSLLSYILPAVT
                     ALVAGYVEFQGAEVAAQRLMDLLLVEAEEENQRPQNTGEARTEAPDALQTADGPVTGR
                     RLPSGKTDRIHPAVQGHTAEHRSGAPLPPFHIRGLSFAWPKSPPLLQEVTMTIPPGRI
                     TGLWGPSGAGKSTLVQLLQRKYTPDAGAILMEDTPINQLNLAGLRSRIGVVPQQIKIF
                     NATLAENLLLGRTAGSLQEVIDRLDELGLASLPGRFGHGLLTLLGEEGRKLSGGETQL
                     LALARALYGRPQLLIIDEGFSAIDVELEQTLSAITAAWARRHAVLLITHNLDSLRRSD
                     YVYLLRGGRIEEEGVPEELIDAGGLFQQLVLIKSRAACARVGSEA"
     gene            complement(2768..>3065)
                     /locus_tag="PKM23_14135"
     CDS             complement(2768..>3065)
                     /locus_tag="PKM23_14135"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNY92659.1"
                     /translation="LIRKHLADPQFCVKRLALMLRISTSHLRKIVHQLSHTSPQKLIE
                     EMRMRLAVQLLEQQFKIHTIARTVGYNSDRAFRAAFRRWQGSSPIQYKKMASAQ"
BASE COUNT          561 a          916 c         1065 g          523 t
ORIGIN      
        1 gcgaaatccg ataaagagag gaatcgtcac atatccccag acgctgatcc ttttcaggag
       61 atcaaagatc gcatcgatgc gctgcacctc gagctgctcc ttgagcagcg agtggtagag
      121 atcccgcgtc atcacctggg actgataaaa ccaggtcagg atggcttcac acattaccag
      181 cagcacaaag aggtgccatc cccgcaggtg gaaagacgtc agcagccggc ggccgggcag
      241 ctgtatgggc cgcggcaggt gcactgaatg ggtcgtcatg cttcgctcct cgcgctgcgc
      301 ggccggtcag gccggttcac gccttgatgt aaggatcggc tcgtcatgcc tcgctcccga
      361 cgcgggcgca ggccgcccgg ctcttgatca gcaccagctg ctggaatagc ccgcccgcat
      421 cgatcagctc ctccggcacc ccctcctcct cgatccgtcc tccgcgcaaa agatagacgt
      481 aatcgctgcg ccgcagcgag tcgaggttgt gggtgatcag cagcacggcg tggcggcggg
      541 cccaggcggc cgtgatggcg gagagcgtct gctccagctc gacatcgatg gcgctgaatc
      601 cctcgtcgat gatgagcagc tgcggccgcc cgtagagcgc ccgcgccagg gcgagcagct
      661 gggtctcgcc gcccgagagt ttgcggccct cctcaccaag cagggttaaa aggccgtggc
      721 caaaacgccc gggcagcgac gcaagaccca gttcatccag acgatctatc acctcctgca
      781 gcgaaccggc cgtgcgcccg agcaggaggt tctccgccag ggtggcgttg aagatcttga
      841 tctgctgcgg caccacgccg atgcggctgc gcaggccggc caggttgagt tggttgatcg
      901 gcgtatcctc catcaagatg gcgcccgcgt cgggagtgta cttgcgctgg aggagctgca
      961 ccagggtgct cttgccggcg ccgctcggcc cccagagtcc ggtgatgcgg ccgggcggga
     1021 tggtcatggt gacctcctgc agcaggggag gcgatttggg ccaggcgaag gagaggccgc
     1081 ggatatgaaa cggggggaga ggcgcgccgg agcggtgctc tgcagtgtga ccttgcacgg
     1141 cgggatgaat acgatccgtt tttccggatg gcagcctgcg gccggtgaca ggcccatccg
     1201 ctgtctgcag ggcatcgggc gcctccgtcc gtgcctctcc cgtattttgt ggtctctgat
     1261 tttcctcctc cgcctcgacc agcagaagat ccatcagccg ctgcgccgcc acctccgcgc
     1321 cctggaactc gacatagccg gccaccagcg ccgtgacggc gggcaggata tagctgagca
     1381 gggagtaggc ggccatgaac tggccgagca gcagctctcc cgcgatcacc tgccgggcgc
     1441 cgagggcgag cagcgtcacc gaaataaagg ccccgagcag gcctacggcc agggtcagtc
     1501 ccgcctgcat cagtccgcgg cgcgcgatgt ggtcctgaaa gaggccgaag aggctctggt
     1561 tgaggcgggt gaaggccggg gcggcgctga agctcatgat ctccggcatg ccctggatgc
     1621 tgtcgatgta ggcggcctcg acgccggcgt ggctcttcat cacctcgcgc tgctgttttt
     1681 tcaggcggct ggcctggcgc cagagcacgg cggtgtaaaa ggggagcatg gccagggtga
     1741 gggcgccgag cagggaggag aaccagaaca tgaagaggag ggagccggcg accagcatga
     1801 ggtcgagaag gaggctctgc atcagcagca ggcaggtgcg gtggatgcgc agactgtcgt
     1861 cgatgcggga ggtgatgtcg ccggtgcggc gggtgtcgaa aaaggatttg ggcaggcgga
     1921 agaggtggag gaggaaatcg ccggtcaccg ccaggctgag gcgtttgttg gcggtgacga
     1981 ggaggcggtc gcggaggtag ccgatgccgg cgcggagggc caatagcagc aaaagtgccg
     2041 aggcgatgaa gaggaggttg tcaagatcct tcgccgggat gagccggtcg atgagggtct
     2101 ggacgaagag agcggtgagc aagcccgcgg ccgtgtagag caggccggtg aagaggatct
     2161 gctgcagcca ggactgctgg ctgcgcagat agccgccgat ccagcgcagc caggagcggc
     2221 tctcttcgtg gagcagggcg ttgttgggtg tgtttgccgg ctgcgccgcc gtgtgcggcg
     2281 cgccgtccgg ttcgagcagg atgacggcat gttcccgcca cattttttca aactccctgc
     2341 gctcgagttt gcagcggccc ttgccggggt cgccggcgag cagccgctcc ccctcaattt
     2401 tatagatgac gagaaagtgg ctcatgccat ccggcaggat gacatgggcg atacagggca
     2461 gcttttcctt cgccagctgc tcgaacgagc cgcgggcgcc ctgcgccttg aagcccatcg
     2521 cccgggcggc ccggaccata tccagcatgg tcgtgccctg aatgtcggtc cggcagagct
     2581 gccgcgcatg gacgagagtc gtgtcgccgc ggtaaaagcg cagcacggaa aggagggctg
     2641 cgggtccgca gtcgagctgg tcgtactggc gaacgagggg aaatctggac cggattctca
     2701 tctcgcctcc ggttgatcac gcgatgtgct gcgccgccaa cagcaagggc ggcggagtct
     2761 caacctctca ttgggcgctc gccattttct tgtactggat cggcgaactt ccctgccacc
     2821 ggcgaaaggc cgcacggaaa gcccggtcgg aattatagcc gacagtgcgg gcgatagtgt
     2881 ggatcttgaa ctgctgctcc agcaactgga cagcgagcct catccgcatc tcctcgatca
     2941 gcttttgcgg cgaggtgtgc gagagctggt gcacgatttt gcgcagatgc gacgtcgaga
     3001 tccgcagcat gagcgccagc cgcttgacgc agaactgcgg atccgccaga tgcttcctga
     3061 tcaaa
//
LOCUS       DAOPWU010000393         3059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_32945_length_3059_cov_4.322237, whole genome shotgun sequence.
ACCESSION   DAOPWU010000393 DAOPWU010000000
VERSION     DAOPWU010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32945_length_3059_cov_4.322237"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PKM23_14140"
     CDS             complement(<1..1080)
                     /locus_tag="PKM23_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92660.1"
                     /translation="MFIYGILAASRRSLLARLRTWATLLLPVVAFLVITALGGLPAHL
                     DRLPDADQILVFFKEPFVLFCLICLGWFISRMQAAPLRWEEFKYFNSLPLGHRQLYYH
                     FLLSDYKTSLWAPHSISAVYAGLVVVAPWPYLLRLATACFLIHLLALLFSHALHLLWM
                     TRPSERRNPVYRAESLITVVTIVLFAFLALAGLLFPQLISGANFWPLVIGSAIAAMVS
                     VHANTHLFGVWLRTNNLFRAPMLAQATYRQRSSRWLQPLRDRNPWLWSHLLLVQRRRQ
                     KGLVVLTMTFVFLSYLASMNNRIWSDRSGVLLAIALSYAALFSYQVTRQMGTEGESVP
                     RLYALPVETRSWLASMTVPALVWLLL"
     gene            complement(1173..1382)
                     /locus_tag="PKM23_14145"
     CDS             complement(1173..1382)
                     /locus_tag="PKM23_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92661.1"
                     /translation="MKRYVALLINHIHKENNILFPMADRVLDSQLDAEIAEKFEKLEE
                     EEIGEGKHEEYHRLLKQLKESYLAD"
     gene            complement(1428..1850)
                     /locus_tag="PKM23_14150"
     CDS             complement(1428..1850)
                     /locus_tag="PKM23_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HNY92662.1"
                     /translation="MEPQLLSDPQQYPTEEIIFSHIGKSKVLWIAFFDHLRTEHPDIT
                     PEWRYYNDGKSWLMKAEAKKKTVFWLSIVEGGFRVTVYVNARQAPEVMAAEIPDEFKA
                     QYQQGIKAGKLTGWTILFQNEKDLQDIRALIALKKRLK"
     gene            complement(1937..2500)
                     /locus_tag="PKM23_14155"
     CDS             complement(1937..2500)
                     /locus_tag="PKM23_14155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY92663.1"
                     /translation="MFTTEEVTAAHWPKVEELFGERGACGGCWCQAWRIEKGEKWEEV
                     KGAVAKERFRRGIEAGTILGILAFEGGTPVGWCTFGPRRSFPLLDRTRAFQCEDADEV
                     WSIPCFYVPRPQRNRGIAHALLAAALAALKARGARIAEGYPAKPNRDGAYISAFAWTG
                     TDSLFEKAGFVMAGDPARSKRRVRKRL"
     gene            complement(2520..>3059)
                     /locus_tag="PKM23_14160"
     CDS             complement(2520..>3059)
                     /locus_tag="PKM23_14160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92664.1"
                     /translation="GAPFTAAPAVALIDPLGRQSTLAGAIPVEGGWQLAIPTDGLTAG
                     RYYLAVEGSVRDFDGSAIPLSCRTGFIVTGGTAVECPQAGAVPSTPELLRSYPNPFNS
                     STMIRYSLAAPAEVRLSIHDLQGREVAVLRRGREAAGSYSVRWQGADGAGRPLPSGLY
                     FAVLEAGGQRRVCKMVMMK"
BASE COUNT          650 a          913 c          948 g          548 t
ORIGIN      
        1 gaggagcagc cagaccaggg cgggaaccgt catagaggcg agccaggagc gcgtctcgac
       61 cggcagggca tacaggcgag gtaccgattc gccctcggtt cccatctgac gcgtgacctg
      121 atatgaaaac agcgcggcat agctcaaagc gatcgccagc agcacaccgg atcggtcact
      181 ccagatgcgg ttattcatcg aggctagata ggagaggaag acaaaggtca ttgtcagcac
      241 aaccagacct ttttgccgcc ggcgttgtac taaaagcaga tggctccaca accagggatt
      301 gcgatcgcga agcggctgca gccagcggga ggagcgctga cgataggtcg cctgtgccag
      361 catcggagcg cggaacaggt tgttggtgcg cagccacact ccgaacagat gggtatttgc
      421 atgtacggaa accatcgccg cgatcgcgct tccgatgacc agaggccaaa agttcgcccc
      481 tgaaatcagc tgcggaaaaa gcagaccggc cagcgccaga aaggcaaaca atacgatcgt
      541 cacaacagtg atcagggatt ccgcccggta gacaggattt cgcctttcag acggacgggt
      601 catccacagc agatggaggg catgggaaaa caacagggcg agcagatgaa tgagaaaaca
      661 agcagtggcc agacgcaaca ggtaaggcca gggcgccacg acaacaagcc ctgcgtatac
      721 agcggagata gagtgaggcg cccagagcga ggtcttgtag tcggacaata aaaaatgata
      781 gtagagttgc cggtggccca gcggcaggct gttgaaatat ttaaactctt cccagcgcag
      841 cggcgcggct tgcatccggc tgatgaacca gcccagacag atcaggcaaa agaggacaaa
      901 aggctccttg aaaaagacga gtatctgatc cgcgtcgggc aggcggtcaa gatgggcagg
      961 gagaccgccg agggctgtga tgacaaggaa agcgactacc ggaagcaaaa gcgtggccca
     1021 ggtccgcaac ctggccagca gggatcgccg gctggcggcg agaattccat agatgaacaa
     1081 aaaaatatct ccaatcaagg tgacaagtaa agagttaaat gacgggggcg aggatccggc
     1141 gggcgccaga gagccgggcc cgccggaccg gttcaatctg cgagatagct ctctttcagc
     1201 tgcttgagca ggcggtgata ttcttcgtgc ttgccctctc cgatctcctc ctcctccagc
     1261 ttttcgaact tttcggcaat ctccgcatcg agctgcgaat ccagcacccg gtcggccatc
     1321 ggaaagagaa tattgttctc cttgtgaatg tggttgatca acagcgccac gtatcgtttc
     1381 atctcctctg cgatggcatt accccatgat cgaacttgtt tgcatcccta tttcagcctt
     1441 tttttaagcg caatcaaagc ccggatgtcc tgcaagtctt tttcattctg gaacaggatc
     1501 gtccaaccgg tgagcttgcc agctttgatg ccctgctggt actgcgcttt gaactcatcc
     1561 ggaatctccg ctgccatcac ctccggcgcc tgtctggcgt tgacatagac cgtgacccga
     1621 aagcccccct cgacaatcga cagccagaag acggtctttt tcttcgcctc cgctttcatc
     1681 agccagctct tgccatcgtt ataatagcgc cactccgggg tgatgtcggg gtgctccgta
     1741 cggaggtgat caaagaaggc gatccacagg accttggact tgccgatgtg ggaaaagatg
     1801 atctcctcgg tgggatactg ctgcggatcg gacagaagct gtggctccat gttttttctc
     1861 cagggttgat gcggcgttga tcgcctccgg ccgggcaggc tgccggcatc ggggtgcatt
     1921 gtgatactgt aatattttac agccgcttgc gcacccggcg cttgcttctg gccggatcgc
     1981 cggccatgac aaagccggct ttttcaaaga gggaatcggt gccagtccag gcaaaggcgg
     2041 agatgtaggc gccgtcgcgg ttgggtttgg ccggatagcc ctcggcaatg cgggccccgc
     2101 gcgccttcag cgccgccagg gcggcggcga gcagggcatg ggcgatgccg cggttgcgct
     2161 gcggccgcgg cacgtaaaaa cagggaatcg accagacctc atccgcatcc tcgcactgga
     2221 aggcgcgggt gcggtcgagc agggggaagg agcgccgcgg cccgaaggtg caccagccga
     2281 ccggcgtgcc gccctcaaag gcgagaatgc caaggatggt ccccgcttcg atgccgcggc
     2341 ggaaccgctc tttggcgacc gcgcccttga cctcctccca cttttcgccc ttttcgatgc
     2401 gccaggcctg gcaccagcag cccccgcagg ccccgcgctc gccgaacagc tcctccacct
     2461 tgggccagtg cgccgcggtg acctcttcgg ttgtgaacat gaccgtcctc atcctccgct
     2521 tatttcatca tcaccatctt gcacacccgg cgctgtccgc cggcctcgag caccgcgaaa
     2581 tagaggccgc tcgggagcgg cctcccggcg ccgtcggcgc cctgccagcg tacactgtac
     2641 gaacccgccg cttcacggcc gcggcgcagc acggcgactt cgcgcccctg cagatcatgg
     2701 atggagaggc gaacctccgc gggggccgcg aggctgtagc ggatcatggt cgaggagttg
     2761 aaggggttgg ggtagctgcg cagcagctcc ggtgtggagg gaactgctcc tgcctgcggg
     2821 cactcgacgg cggttccgcc ggtgacgata aagccggtgc ggcagctgag ggggatcgcg
     2881 ctgccatcga aatccctgac gctgccttcc accgcgaggt aataacggcc ggcggtcaat
     2941 ccatccgtgg ggatggcgag ctgccagccc ccctcgacag ggatcgcgcc cgccaaagtg
     3001 ctctgccggc cgaggggatc gatcagggcc acggccggcg ctgcggtaaa gggcgcgcc
//
LOCUS       DAOPWU010000394         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_32962_length_3058_cov_4.904762, whole genome shotgun sequence.
ACCESSION   DAOPWU010000394 DAOPWU010000000
VERSION     DAOPWU010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32962_length_3058_cov_4.904762"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /gene="ygeW"
                     /locus_tag="PKM23_14165"
     CDS             complement(<1..916)
                     /gene="ygeW"
                     /locus_tag="PKM23_14165"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HNY92665.1"
                     /translation="MDLIESNLLALKQLEFDSYNQDFLLTWEKSRDEIEALLLTAEIF
                     AEMHRLKKPFRLFDSGLAISIFRDNSTRTRFSFASAVNALGLGLAELDEVKSQIAHGE
                     TVRETATMIAFLAEAIGIRDDMFLGEGNRYMREVSAALREGFEAGVLHQHPAVINLQC
                     DIDHPTQTAADLLHLKKHFGSLEALRGKKIAMTWAFSPSYGKPLSVPQGIIGLLTRFG
                     MEVTLAHPPGYDLIPEVVELARENSQSSGGRFHQAESMEEAFAGADIVYPKSWAPYKV
                     MEERTALLKQGNREGLKALEQTCLANNAR"
     gene            complement(1134..1598)
                     /locus_tag="PKM23_14170"
     CDS             complement(1134..1598)
                     /locus_tag="PKM23_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNY92666.1"
                     /translation="MKLTVNGVTHEIDAGLAEMRLLDWLREELGLTGAKAGCEIGACG
                     ACTVLVDGRPVKSCTRRLRDVAGKSVLTIEGLTPLDGSLHPLQQAFLDAGAVQCGFCT
                     PGMILAAHALLLRNPAPARAEIRQALQGNLCRCTGYQQIIDAVELAAKSYQK"
     gene            complement(1595..>3058)
                     /locus_tag="PKM23_14175"
     CDS             complement(1595..>3058)
                     /locus_tag="PKM23_14175"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNY92667.1"
                     /translation="YKRHPYKVDYKMGLTREGRITAVDCRIIADGGAYCSVTPWVTWR
                     STVQCCGPYVVENVHCDTFGVYTNNPVTGAMRGFGSPQMNFVIESMIEEAAEKLGLTG
                     IALRRMNLVRQGSTTITGQKLEGHTVAMEQVLDKVLEEIDYQNKLARCSHGRGNEEEL
                     YGIGLAMSYRGVSLGAEGVDFCAAIVNVQADGSVLLEAGVHENGQGAEAAMSLILARE
                     LGIDVDKIRYRRSSTSVIPDSGTTVASRGTIMGGGAVALAAQELKQKIAVFAAPELAC
                     APEEISFTSNQIIGRGGRSLPFAEVIRGMFQKQQFPYALGVYRAPQVSWDEETGQGKA
                     YFTWVYGCQAVELTVNRKSGKVRLLNAVACHDVGKAINNTMLLGQYYGGMAMGTGYAL
                     HEDLHIDGGRIGHTNLNRYRIPRSTDLCEMRAIVVENHDPVSPTGAKSIGEPSNEIMA
                     PAIANAIHHATGVRYYHLPIRIAGTDVPEEAQKRGQL"
BASE COUNT          509 a          963 c          947 g          639 t
ORIGIN      
        1 agcgcgcgtt attggccaga caggtctgtt ccagcgcctt gagcccctcg cggttgcctt
       61 gtttgagcag cgcggtgcgc tcttccatga ctttgtacgg ggcccaggat tttggataga
      121 cgatgtcagc gcctgcaaag gcctcttcca tgctctccgc ctggtgaaaa cggccgccgc
      181 ttgactggct gttttcgcgg gcgagttcga ccacctcggg gatgagatca tatcccggcg
      241 gatgggcgag ggtcacctcc attccgaagc gggtgagcag gccgatgatg ccctgcggca
      301 ccgagagggg cttgccgtag ctgggggaga aggcccaggt catggcgatt tttttgccgc
      361 gcagggcctc gagcgaaccg aaatgctttt ttaaatggag gagatcggcc gcggtctggg
      421 tgggatggtc gatgtcgcac tggaggttga tgaccgcggg atgttgatgg aggacccccg
      481 cttcgaagcc ctcccggagg gcggcgctga cctcgcgcat atacctgttg ccttcgccga
      541 ggaacatgtc gtcgcggatg ccgatcgctt cggcgaggaa ggcgatcatc gtggcggtct
      601 cgcgcacggt ttcgccgtgg gcgatctggg acttgacctc gtcgagttcg gcgagtccga
      661 gtccgagggc gttgacggcg gaggcgaagc tgaagcgggt gcgggtcgag ttgtcacgga
      721 agatggagat cgccagtccg gagtcgaaga ggcgaaaggg tttcttcagc cggtgcatct
      781 cggcaaaaat ttccgccgtg agcaggaggg cctcgatctc gtcgcggctc ttttcccagg
      841 tgaggagaaa gtcctggtta taggaatcga actccagttg tttgagcgcc agcaggttgc
      901 tttcgatcag atccattttc ctcctctttg cggtttatca agtcatttca ctgtggcaat
      961 gaatcacaaa aattatctct cacggagcca cggaggcaca aagagaggac atcatcagcg
     1021 caaagcgctt ttaaacagtg cctctttgct ctctcttttc tttgtgtcgc cgtgccttgg
     1081 tgagagatgg ctttttcttg tttctctctg cgtctctgta cctctgcgcg agattatttc
     1141 tgatagcttt tcgccgccag ctcgacagcg tcaatgatct gctggtagcc cgtgcagcgg
     1201 cagagattgc cctgcagggc ctggcggatc tcggcgcgcg ccggcgcagg attccgcagc
     1261 agcagcgcat gcgccgccag aatcattccc ggcgtgcaga atccgcactg caccgccccg
     1321 gcgtcgagaa aggcctgctg caggggatgc agcgagccgt ccagcggcgt cagtccctcg
     1381 atcgtcagga ccgattttcc cgccacatcc ctgagccgcc gcgtgcagga cttgaccggc
     1441 cggccgtcga ccagcaccgt gcaagccccg caggcgccga tttcacagcc cgccttggct
     1501 ccagtcagtc cgagttcctc gcgcagccag tcgagcagcc tcatttcggc aagccctgcg
     1561 tcgatctcgt gggtcacacc gttcaccgtc agcttcatag ctgacctcgc ttctgcgctt
     1621 cttcgggcac atccgttccg gcaattcgga tcggcagatg gtaatagcgg acgcccgtgg
     1681 cgtgatggat cgcgttggcg atcgccgggg ccatgatctc gttggacggc tcgccgatgc
     1741 tcttggcgcc cgtaggcgag accggatcgt gattctcgac cacaatcgcc cgcatctcgc
     1801 acaggtccgt cgaccggggt atccggtagc ggttcagatt ggtatggccg atccggcccc
     1861 cgtcgatgtg gagatcctcg tgcagggcat agccggtgcc catcgccatg cccccgtaat
     1921 attgcccgag cagcatggtg ttgttgatcg ccttgccgac atcgtggcag gccacggcat
     1981 tgagcaaccg caccttgccg ctcttgcggt ttaccgtcaa ctcgacggcc tggcagccgt
     2041 agacccaggt gaaataggcc ttgccctggc ccgtctcctc gtcccagctc acctgcggag
     2101 cgcggtagac cccgagggca tagggaaact gctgcttctg gaacatcccc ctgatcacct
     2161 ccgcaaaggg cagggagcgg cctcccctcc caatgatctg gttggaggtg aacgagatct
     2221 cctcgggggc gcaggccagc tccggggcgg cgaaaacggc gattttttgc ttcagctcct
     2281 gcgccgccag agcgacagct cctccgccca tgattgtgcc gcgtgaggcg acggtggtgc
     2341 cgctgtccgg gatcaccgaa gtggaggagc gccggtagcg gatcttgtcc acgtcgatcc
     2401 ccagctcccg tgccaggatc agcgacatcg ccgcctcggc gccctggccg ttttcgtgca
     2461 cccccgcctc gagcaggacc gatccgtccg cctggacgtt gacgatggcg gcgcagaaat
     2521 cgactccctc ggccccgaga ctgaccccgc ggtagctcat cgccaggccg atgccgtaga
     2581 gctcctcctc attcccgcgc ccatggctgc agcgggcgag cttgttctgg taatcgatct
     2641 cctccagaac cttgtccagc acctgctcca tcgcgaccgt gtgcccctcg agcttctggc
     2701 cggtgatggt ggtgcttccc tgtcgcacca ggttcatgcg gcgcagcgcg atccccgtca
     2761 ggcccaactt ttccgccgcc tcctcgatca tcgattcgat gacgaaattc atctgcggcg
     2821 agccaaatcc ccgcatcgcc ccggtgaccg gattgttggt atagaccccg aaggtgtcgc
     2881 agtggacgtt ctcgactaca taggggccgc agcactgcac ggtcgagcgc caggtcaccc
     2941 agggggtgac cgaacagtag gccccgccgt cggcgatgat gcggcagtcg actgccgtga
     3001 tccgcccctc ccgggtcagc cccatcttgt agtcgacctt gtagggatgg cgtttgta
//
LOCUS       DAOPWU010000395         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_33034_length_3054_cov_3.937646, whole genome shotgun sequence.
ACCESSION   DAOPWU010000395 DAOPWU010000000
VERSION     DAOPWU010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33034_length_3054_cov_3.937646"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKM23_14180"
     CDS             complement(<1..182)
                     /locus_tag="PKM23_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92668.1"
                     /translation="MENRTRTLFKRLQAGRLIALLSPARPEDCLAAWEALAPLGITLE
                     IALRSPAAAPGIALLL"
     gene            424..1917
                     /gene="malQ"
                     /locus_tag="PKM23_14185"
     CDS             424..1917
                     /gene="malQ"
                     /locus_tag="PKM23_14185"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNY92669.1"
                     /translation="MELTRGSGILMHVTSFPSPHGIGDLGQEAYSFIDRLAESRQKYW
                     QLLPLNPTDPAYGNSPYLSSSAFALNSLLISPERLIEEGLLTPADFEGLTPPAAGPID
                     YPAVSNYKRGLFARAFARWRTSPDPGFERFCADQAAWLEDFALFIALKGHFPGQMWSE
                     WPVDLRDRHPEALARMRTELAEAIHFEKFLQFVVQRQWFDLKRHCASRGITIIGDLPI
                     YVDFGSSDVWSHPDRFKLDENREPYVVSGVPPDYFSATGQLWGNPIYDWDALAKDGFA
                     WWLNRMRQNLRLFDIVRIDHFRGLIAYWEVPAEEETAINGRWVTVPTYPFFERLVAEI
                     PNFNVIAEDLGVITDDVKEALHRFGLPGMKVLLFAFSGNPAENPYMPWRYEPNCIVYT
                     GTHDNNTTIGWWRLDATEEEKRHLQEYLGRAVDESSVHWELIRVAMSSPARVAIIPLQ
                     DLLGVGERGRMNVPGTNTGNWEWNLGGDRMDPGCFARLAELTIQNNR"
     gene            1973..2782
                     /locus_tag="PKM23_14190"
     CDS             1973..2782
                     /locus_tag="PKM23_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNY92670.1"
                     /translation="MTWFTSLDPILQALLATCFTWLLTALGAAMVFFFKSIDRRVLNS
                     MLGFAAGVMIAASFWSLLAPALEMAEGGPLPAWVPAVLGFLLGGAFLWLVDHILPHLH
                     IGLPDTEAEGIKTHWRRSILLVLAITLHNFPEGLAVGVAFGAVGADLPSASLAGAVAL
                     ALGIGIQNFPEGAAVSVPLRREGLSRTKSFWYGQASGIVEPIAGVLGAASVLLIKPIL
                     PYALAFAAGAMIYVVVEELIPEAQQESDTHAATIGAMLGFAVMMTLDVALG"
     gene            complement(2848..>3054)
                     /locus_tag="PKM23_14195"
     CDS             complement(2848..>3054)
                     /locus_tag="PKM23_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92671.1"
                     /translation="RYELPLPGRVVLRVINVNGREVARLVEGEQRAGIHEVNFRAENN
                     ASGLYWAELQTSEKRVHIKMMLVR"
BASE COUNT          546 a         1040 c          885 g          583 t
ORIGIN      
        1 tcaaggagga gggctatgcc cggcgcagcc gccggggagc gcagggcgat ctcgagggtg
       61 atgccgaggg gagcaagggc ttcccaggcc gccaggcaat cctccggccg ggccggcgaa
      121 agcagtgcga tcagccgccc cgcctgcagg cgcttaaaaa gggtgcgtgt cctgttttcc
      181 atctgttcct cacatgttcc gcatttgcca atggttaaac ataccaaaac agatggaaaa
      241 tcaaaacact ttttttcagt gaacgcggcg ccaggtggga accagatttg ggtgagcggc
      301 gccaggtggg agccagatta gggcgagcgg cgccaggccg ggtgattttc agcttgattg
      361 ctatgcaaaa ttcccctata ctatacggcg aaaaagcagg cgacaatcct atcaggagaa
      421 gagatggaac tcactcgcgg tagtggaata ttgatgcatg tgacctcctt tccctcgcct
      481 cacggtatcg gcgatctggg gcaggaagcc tactccttta tcgaccgtct ggccgaatcc
      541 agacagaaat actggcagct ccttcccctc aaccccaccg atccggccta cggcaactcc
      601 ccctatctca gcagcagcgc cttcgccctc aactccctgc tcatcagccc cgagcggctg
      661 atcgaggaag ggctgctcac cccggccgat tttgagggtt tgaccccgcc ggcggcggga
      721 ccgatcgact atccggcggt ctcgaactat aaacggggac tcttcgccag ggccttcgcc
      781 cgctggcgca cctcccctga ccccggcttc gagcgctttt gcgccgatca ggccgcctgg
      841 ctcgaggatt tcgccctctt catcgccctc aagggccatt tcccgggcca gatgtggagc
      901 gaatggccgg tagatttgcg cgaccgccat cccgaggccc tcgccaggat gcgcacagag
      961 ctggccgagg ccatccactt tgaaaaattt ctccagttcg tcgtgcaaag acagtggttc
     1021 gatctcaaac gtcactgcgc cagccgcggc atcaccatca tcggcgacct gcccatctat
     1081 gtcgatttcg gcagcagcga cgtctggtcc catcccgacc gcttcaaact cgacgaaaac
     1141 cgcgagcctt atgtggtctc cggcgtgccc cccgactatt tcagcgccac cggccagctc
     1201 tggggcaatc cgatctacga ctgggatgcc ctggccaagg acggcttcgc gtggtggctc
     1261 aaccgcatgc gccagaacct gcgcctcttc gatatcgtac gcatcgacca cttccgcggc
     1321 ctcatcgcct actgggaagt gccggccgaa gaggaaactg ccatcaacgg gcgctgggtg
     1381 accgtcccca cctacccctt cttcgaacgc ctcgtcgccg agatccccaa cttcaacgtc
     1441 atcgccgagg acctcggcgt gatcaccgac gatgtcaagg aggccctgca tcgcttcggc
     1501 ctgccgggaa tgaaagtgct cctcttcgcc ttcagcggca accccgccga gaatccctac
     1561 atgccctggc gctatgagcc caactgcatc gtctacaccg gcacccacga caacaacacc
     1621 accatcggct ggtggcgtct tgacgccact gaggaagaga agcgccacct ccaggaatac
     1681 ctcggccgcg ccgtcgacga atccagcgtg cactgggagc tgatccgcgt ggcgatgagt
     1741 tcgccggccc gggtggcgat catccccctc caggacctgc tcggcgttgg cgagcgcggg
     1801 cggatgaatg tgcccgggac caatacaggc aactgggagt ggaatctggg aggcgacaga
     1861 atggatcccg gctgcttcgc acgcctcgcc gaactgacca ttcaaaacaa ccgctgaggg
     1921 agcgcatgag cctgcgcctc ccgcgctgta tccactccct acggaggaga acatgacctg
     1981 gttcaccagc ctcgatccga tcctgcaggc tttgctggcg acctgtttca cctggctgct
     2041 caccgccctc ggcgccgcca tggtcttttt tttcaagagc atcgaccgcc gcgtactcaa
     2101 cagcatgctt ggcttcgccg cgggagttat gatcgccgcc agcttctggt cgctgctcgc
     2161 cccggccctc gagatggctg aaggtgggcc gctgcccgcc tgggtgccgg cagtgctcgg
     2221 cttccttctc ggtggagcct tcctttggct ggtcgaccac attctccccc atcttcatat
     2281 cggtctgcct gatacggagg cggaggggat caagacccac tggcggcgca gcatcctgct
     2341 cgtgctggcc atcaccctgc ataactttcc cgagggtctg gctgtcggag tggccttcgg
     2401 ggcggtcggc gccgatctgc cttccgcctc gctcgccggg gcagtcgccc tcgccctcgg
     2461 catcggcatc cagaacttcc ccgagggggc ggcggtttcg gtcccccttc gccgtgaagg
     2521 gctcagccgt accaaaagct tctggtacgg ccaggcctcg ggcatcgtcg aacccatcgc
     2581 cggtgtgctc ggggcggcct ccgtcctgct catcaagccg atcctgccct acgccctcgc
     2641 cttcgccgcg ggcgcgatga tctatgtggt ggtggaggag ttgattccgg aagcccagca
     2701 ggagagcgac acccacgccg cgaccatcgg cgcgatgctg ggatttgcgg tgatgatgac
     2761 cctggatgtg gccctgggat aagactcaat taaggaaagc cccggcgcgc acaccaggct
     2821 cagccggggc agttggcttg ctggcgctca gcgcaccagc atcatcttga tgtgcacccg
     2881 cttttcgctg gtctgcagtt ccgcccagta gaggccgctg gcgttgttct ccgccctgaa
     2941 attcacctca tggatgccgg cgcgctgttc cccctccacc agccgcgcta cctcccgccc
     3001 gttgacattg atcacccgca gcaccacccg gccaggcagc ggcagttcgt agcg
//
LOCUS       DAOPWU010000396         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_33064_length_3052_cov_4.147147, whole genome shotgun sequence.
ACCESSION   DAOPWU010000396 DAOPWU010000000
VERSION     DAOPWU010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33064_length_3052_cov_4.147147"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PKM23_14200"
     CDS             complement(<1..538)
                     /locus_tag="PKM23_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY92672.1"
                     /translation="MRSRIYAILRKEFIHILRDPRSLVIVFLLPILMVLLYGHAITFD
                     IRDIRLGLLDQDRTPAARELVEGLVSSNYFRLTGVLESREEIGEAFLHRRMVAVLIIP
                     QGFAAALGSQPQSEVQVIIDGSNANTATVAMNYLKAYFVTRSLQLNAQVMRTPIDLQP
                     RVWYNPDLKSVNFIVPGLV"
     gene            complement(535..1308)
                     /locus_tag="PKM23_14205"
     CDS             complement(535..1308)
                     /locus_tag="PKM23_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY92673.1"
                     /translation="MDNPYSVTVEELSRRFGDFVAVDRVTFSVRDGEIFGFLGANGAG
                     KTTVIRMLCGLLLPTSGKARVAGHDIYTESGRIKEKIGYMSQKFSLYDDLTVAQNLEF
                     YGGIYGLGRAELGRAITAAIATTDLGAHLGKLTRDIPAGWKQRLALSCAILHRPKLLF
                     LDEPTGGVDPISRRAFWGLIYDLAEQGTTVFVTTHYMDEAEYCNRLSIMHAGRLIAIG
                     SPGELKARYHKRSIEELFIDLVRGGNPGQPAEEEEAAKQ"
     gene            complement(1317..2234)
                     /locus_tag="PKM23_14210"
     CDS             complement(1317..2234)
                     /locus_tag="PKM23_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY92674.1"
                     /translation="MVYDIRISGLCKSFGAVTALDGLDLTVEEGEMLGLIGPDGAGKT
                     TALRILCGLLLAGGGSVRVAGLDPGRETRRVREFLGYMPQRFSLYPDLTVAENLRFFA
                     DLFRVPKAEREERMARLLDFSRLGPFTGRLSARLSGGMKQKLALSCTLIHTPKILLLD
                     EPTTGVDPVSRREFWEILGSLKREGVTILVTTPYMDEAARCDRVAFIHRGTLLTVAPP
                     AEIPKLYRKGLIEVRSGDNLAAARALRASGRFESVQVFGDRIHLSAPEDSAELERSVR
                     TILDQAGIHIDTVEPVEAGIEDVFVELLK"
     gene            complement(2246..>3052)
                     /locus_tag="PKM23_14215"
     CDS             complement(2246..>3052)
                     /locus_tag="PKM23_14215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNY92675.1"
                     /translation="TGSGTLEATEVLVSAKSGGTLTALLVSEGDAVTAGQLIAAIDSE
                     KVFLQKRQALAALEELRLNRLNAGRLARTARENLDNLAKKSERIGALLDEGSATQQQY
                     DDLETGRKAAEAQYENARTSLAALDAREEQLLAQIGLVESQLRDARVTAPISGVVINK
                     YLEAGEVARPLAPVATIADLSRMHIRIYLKESGIARIRLNGGAELSVTAWPEKKFPGR
                     IAWISDKAEFTPKNIQTKEARSDLVYAVKVEVANPEGILKIGMPADVTLL"
BASE COUNT          534 a          962 c         1007 g          549 t
ORIGIN      
        1 ccaccaggcc ggggacgata aagttgacgc tcttgagatc ggggttgtac cagacccgcg
       61 gctgcaggtc gatcggcgtc cgcatcacct gtgcattgag ctggagggag cgggtgacga
      121 agtaggcctt gaggtaattc atcgccaccg tggccgtgtt ggcgttggag ccgtcgatga
      181 tcacctgcac ctcactctgc ggctggctgc ccagggcggc ggcgaaaccc tgcgggatga
      241 tcagcaccgc gaccatacgg cggtggagaa acgcctcccc gatctcctcg cggctctcca
      301 gcaccccggt cagcctgaaa tagttcgagc tgaccagccc ctcgaccagc tcgcgcgcgg
      361 ccggagtacg gtcctggtcg agcagcccca gccggatatc gcgaatgtcg aaggtgatgg
      421 catggccgta aagcagcacc atcaggatcg ggagcaggaa gacgatcacc aggctgcgcg
      481 gatcgcgcag gatgtggatg aactccttgc gcaggatggc atagattctc gatctcattg
      541 ctttgcagcc tcctcttcct ccgccggctg tccggggttc ccgcccctca ccagatcgat
      601 gaagagttcc tcgatgctcc gcttgtggta gcgcgccttg agctcgccgg gcgagccaat
      661 cgcgatcaga cggccggcgt gcatgatcga aaggcgattg cagtattcgg cctcatccat
      721 gtagtgggtg gtgacaaaaa ccgtggtccc ctgctcggcc agatcgtaga tcagtcccca
      781 gaaagcgcgg cgcgagatcg gatcgacccc gccggtaggc tcgtcgagga agaggagttt
      841 gggccggtgg aggatggcgc aggagagggc caggcgctgc ttccagccgg cgggaatatc
      901 ccgggtgagc ttgccaagat gggccccgag atcagtggtg gcaatggcgg cggtgatcgc
      961 ccggccgagt tccgcccggc ccagaccgta gatgccgccg tagaactcga ggttctgggc
     1021 cacggtgagg tcatcatata gcgaaaattt ctgcgacatg tagccgatct tttccttgat
     1081 gcggccgctc tcggtgtaaa tatcatggcc ggccaccctc gctttaccgg aggtcggcag
     1141 gagcaggccg cagagcatgc gaatgaccgt ggtcttgccc gccccgttgg cgccgagaaa
     1201 gccaaagatt tcgccgtcgc gcacggaaaa agtgacccgg tcgacggcga caaaatcacc
     1261 gaagcggcgg ctgagctctt ccactgttac tgaataaggg ttatccatag aaccgtctat
     1321 ttcaagagtt cgacaaagac atcctcgatg ccggcctcga ccggctcaac tgtatcgatg
     1381 tgaatcccgg cttggtccag aatagtgcgg acggagcgct ccagctccgc cgaatcctcc
     1441 ggcgcgctca gatggatgcg gtcgccgaag acctgcacac tctcaaagcg gccgctggcg
     1501 cgcagggctc gggccgccgc caggttatcc ccgctgcgca cctcgatcag ccccttgcga
     1561 tagagttttg gaatctccgc cgggggtgcg accgtgagca gagtgccccg gtggataaag
     1621 gcgacgcggt cgcagcgcgc cgcctcgtcc atgtaggggg tggtgaccag gatggtcacc
     1681 ccttcgcgct tgaggctgcc gaggatctcc cagaattccc ggcgcgagac cggatccacc
     1741 ccggtggtcg gctcatccag cagcaggatc ttcggggtgt ggatcagggt gcaggagagg
     1801 gcgagctttt gcttcatacc gcccgagagc cgggcgctga ggcggccggt gaaggggccg
     1861 agccggctga aatcgagcag ccgcgccatg cgctcttcgc gctccgcttt cggcacccgg
     1921 aagagatctg cgaaaaaacg caggttttcc gccacggtga ggtccgggta gagggagaag
     1981 cgctggggca tatagccgag aaactcacgc acccggcgcg tctcccggcc gggatccaga
     2041 ccggcgacgc ggacactgcc ccccccggcg aggagcagac cgcagaggat gcgcagggct
     2101 gtagtcttgc ccgccccgtc gggaccgatc agaccgagca tctccccctc ttcgacggtg
     2161 aggtcgagtc cgtcaagggc tgtcaccgcc ccgaaggact tgcacaggcc gctgatgcgg
     2221 atatcgtaga ccacactccc ctccctcaca gcagggtgac atcggccggc atgccgatct
     2281 tgaggatgcc ctccggattg gccacctcca ccttgaccgc gtagaccaga tcgctgcgcg
     2341 cctctttggt ctggatattt ttaggggtaa actcggcctt gtccgagatc caggcaatgc
     2401 ggccggggaa tttcttctcg ggccaggcgg tgacggagag ttccgccccg ccgttgagac
     2461 gaatgcgcgc gatcccgctc tccttgaggt agatgcggat atgcatgcgg cttagatcgg
     2521 cgatggtcgc caccggggcc agggggcgcg ccacctcgcc ggcctcgaga tatttattga
     2581 tcaccacccc gctgatcggg gccgtcacac gcgcatcgcg cagctggctt tcgaccagcc
     2641 cgatctgggc cagcagctgc tcctcgcggg catccagggc ggccagggag gtgcgggcgt
     2701 tctcgtactg cgcctcggcg gccttgcgcc cggtctcgag gtcgtcatac tgctgctggg
     2761 tcgcgctgcc ctcatcgagc agcgccccga tgcgctcgct ctttttggcg aggttgtcga
     2821 ggttctcgcg ggcggtgcgg gcgagccggc cggcattgag gcggttgagc ctgagttctt
     2881 ccagggcggc cagggcctgc ctcttctgta aaaagacctt ttcgctgtcg atggcggcaa
     2941 tgagctgccc ggccgtgacc gcatcgccct cgctgaccag cagggcggtg agggtgccgc
     3001 ccgacttggc gctgaccagc acctccgtcg cctcgagggt gccggagccg gt
//
LOCUS       DAOPWU010000397         3048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_33121_length_3048_cov_4.484464, whole genome shotgun sequence.
ACCESSION   DAOPWU010000397 DAOPWU010000000
VERSION     DAOPWU010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33121_length_3048_cov_4.484464"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1038)
                     /gene="asd"
                     /locus_tag="PKM23_14220"
     CDS             complement(145..1038)
                     /gene="asd"
                     /locus_tag="PKM23_14220"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008204566.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNY92676.1"
                     /translation="MSGRLYFYRRGSDRREEEIILGGGLMRWAYRSRIGRALTSFLFN
                     RRWPSRLAGWYADSRWSRRQIAPVIRNLGIDTAEFADPVGSYPTFNAFFTRRLKPGAR
                     PFSPEDEQVASPADGRVLVYPQLRETTLLPVKGKSFTLDTLLGMPAAGYAGGSLAIVR
                     LCPADYHRFHFPCDARVIEQRRIHGHYHSVNSLVLAMGIGVFDQNERQITRMENSRLG
                     EFALIEVGAFGVGSIVQTFTGDEVRKGEEKGYFQYGGSTIVLLFRQGQVAFCTDLIAH
                     SAEGIETFLKAGQLLARMTAT"
     gene            1276..2160
                     /gene="pdxS"
                     /locus_tag="PKM23_14225"
     CDS             1276..2160
                     /gene="pdxS"
                     /locus_tag="PKM23_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNY92677.1"
                     /translation="MQTIENMRVKIGLAEMLKGGVIMDVTTAEQAKIAEEAGAVAVMA
                     LERIPADIRAHGGVARMSDPTVIKSIQTAVSIPVMAKCRIGHFAEAQVLEALGVDFID
                     ESEVLTPADEEFHVDKWKFKVPFVCGCRNLGEALRRIAEGAAMIRTKGEAGSGNIVEA
                     VRHMRAVQGGIRRLQGLGDEELVTEAKNLGAPVELVREVKKLGKLPVPNFSAGGVATP
                     ADASLMMQLGAETVFVGSGIFKSSDPVKRARAIVSATTHYLDFDLIARESEGLGEAMP
                     GLEISSIPENERMAGRGW"
     gene            2161..2742
                     /gene="pdxT"
                     /locus_tag="PKM23_14230"
     CDS             2161..2742
                     /gene="pdxT"
                     /locus_tag="PKM23_14230"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNY92678.1"
                     /translation="MRIGVLAIQGDYGKHRQMLESLGQEAPLVRTERELKECDGLIVP
                     GGESTTLTILLQKHGLWDPLQAFGRRKAIFGTCAGLILLSREVRDHKLASLNLIDLEV
                     ERNAYGRQIDSFIDRVRLDLRGTPGSMEGVFIRAPRIIACGMEVTPLGWHREEVVAAE
                     NEHIMVSTFHPELTESTLLHRHFLTKVSRFLHD"
     gene            complement(2797..>3048)
                     /locus_tag="PKM23_14235"
     CDS             complement(2797..>3048)
                     /locus_tag="PKM23_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92679.1"
                     /translation="DHEAVQGYTQLNKEHLLVAVCKALGVDMHAHHHHEAGANLTKMK
                     KELKELKKKRDQAISAEDKTTLKRTRTRMRRLKKRLSKS"
BASE COUNT          589 a          897 c          953 g          609 t
ORIGIN      
        1 ttgagtgccg ggttccgcgg cgcctggatt gcagcccgcc ttgcgaatga taacagcatg
       61 gatataaaaa gtattcttat tcgccgggaa ttgcaagcac tatttttgcc ggatccggct
      121 ggtgtgagga tgccacgttt ccgttcaggt ggcagtcatg cgcgccagaa gctgccccgc
      181 tttaagaaag gtctcgatcc cctccgcgct gtgggcgatc aggtctgtgc aaaaagcgac
      241 ctggccttgc ctgaagagca ggacgatggt tgatcccccg tattggaaat aacccttctc
      301 ctcccccttg cgcacctcat cccctgtgaa ggtctggacg atgctgccca caccgaaggc
      361 cccgacctcg atcagggcga actcgcccag ccggctgttt tccatccggg tgatctgccg
      421 ctcgttctgg tcaaagacgc cgatccccat cgccagcacc agggagttga ccgagtgata
      481 atggccgtgg atgcggcgct gttcgatgac ccgcgcgtca cagggaaagt gaaagcggtg
      541 atagtcggcg gggcagaggc ggacgatggc caggctgccc ccggcatagc cggcggccgg
      601 catcccgagc agggtatcga gggtgaagga ttttcccttg acgggaagga gggtcgtctc
      661 ccgcagctgc ggatagacca gcacgcgccc atccgccggt gaggcgactt gttcatcctc
      721 ggggctgaag ggcctcgccc cgggtttgag acggcgggtg aaaaaggcat tgaaggtggg
      781 atagctgccg acgggatcgg cgaattcggc ggtgtcgata cccagattgc gaatgacggg
      841 ggcgatctgg cgccttgacc agcgcgaatc ggcataccag ccggccaggc ggctcggcca
      901 gcggcggttg aagagaaagc tggtcagcgc ccggccgatc ctgctgcggt aggcccatcg
      961 catcaatccc ccgccaagta tgatctcctc ttccctgcgg tcgctgccgc gacgataaaa
     1021 atagagccgc ccggacatga tggatttatc cttcctgctg cccctcgccg tgtatggcgg
     1081 ctccgggcat cttcttagtc ttcacaattg cgaggatgat cctacgaaga tagccatttt
     1141 tcagggattc agcaagagaa aaggctgaaa acctcaccac cgattttttc gctctgaaaa
     1201 gagcagttga taaattgcaa aaaaatacat aactttaata ttggaatcca aagggtaaaa
     1261 atgaggtata tcgttatgca aaccatagag aatatgcgag ttaagattgg cctggccgag
     1321 atgctcaagg gcggggtgat catggatgtg accaccgcgg agcaggccaa aatcgccgag
     1381 gaggccggcg ccgtcgcggt tatggcgctt gagcgcatcc ccgccgatat ccgcgcccat
     1441 ggcggcgtcg cacgcatgtc cgacccgacc gtgatcaagt cgatccagac ggcggtctcg
     1501 atcccggtga tggccaagtg ccgcatcggc catttcgccg aggcccaggt gctcgaggcc
     1561 ctcggtgtcg atttcatcga cgagagcgag gtgctcaccc cggccgacga ggagttccac
     1621 gtcgacaagt ggaaattcaa ggtgcccttt gtctgcggct gccgcaatct gggtgaggcc
     1681 ctgcgccgca tcgccgaggg ggcggccatg atccgcacca agggcgaagc gggttcgggc
     1741 aacatcgtcg aagcggtgcg ccacatgcgc gcggtgcaag gcggcatccg ccgtctgcag
     1801 ggtctgggcg atgaggaact ggtgacagag gcaaagaacc tcggcgcccc ggtcgagctg
     1861 gtgcgggagg ttaaaaagct gggcaaactg cccgtcccca acttttccgc cggcggcgtc
     1921 gccaccccgg ccgatgcctc gctaatgatg cagctcggcg ccgagaccgt ctttgtcggc
     1981 agcggcatct tcaagtccag tgatccggtc aagcgcgccc gggccatcgt cagcgccacc
     2041 acccattacc tcgatttcga cctcatcgcc agagagtcgg agggcctcgg tgaggcgatg
     2101 ccgggtctgg agatctcctc catccccgaa aacgagcgaa tggcggggag aggctggtag
     2161 atgcgcatcg gcgtcctggc gatccagggg gactatggca aacaccggca gatgctcgaa
     2221 tccctggggc aggaggcgcc cctggtgcgc acggagaggg aactgaagga gtgcgacggc
     2281 ctgatcgtgc cgggcggaga gtcgaccacc ctgaccatac tgctgcagaa gcacggcctc
     2341 tgggatcccc tccaggcatt cggccggcgc aaggccattt tcggcacctg cgccgggctg
     2401 atcctgctct cccgtgaggt ccgcgaccac aagctggcca gcctcaatct gatcgatctg
     2461 gaagtggagc gcaacgctta cggccggcag atcgactctt tcatcgatag ggtccgcctc
     2521 gacctgcgcg ggacgccggg cagcatggaa ggtgtcttca tccgcgcccc gaggataatc
     2581 gcctgcggca tggaggtcac tcccctgggc tggcatcgcg aggaggtggt ggctgcggag
     2641 aacgagcata tcatggtcag cacctttcat ccggaactga ccgagagcac ccttcttcac
     2701 cgtcattttc tcaccaaggt cagccgtttc ctgcacgatt gacggcggta ggtaaaagaa
     2761 aagcccgggt ctgctgcggc agcccgggct tttcttttag cttttgctga ggcgtttttt
     2821 cagccgcctc atcctggtac gggttcgttt gagggtggtt ttgtcctcgg ctgaaatcgc
     2881 ctgatcgcgt tttttcttca attctttcag ttcttttttc attttcgtaa ggttggcgcc
     2941 ggcctcgtga tggtggtggg catgcatatc cacccccagg gccttgcaca cggctaccag
     3001 caggtgttcc ttgttgagct gggtgtagcc ctggaccgct tcatgatc
//
LOCUS       DAOPWU010000398         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_33363_length_3033_cov_5.744795, whole genome shotgun sequence.
ACCESSION   DAOPWU010000398 DAOPWU010000000
VERSION     DAOPWU010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33363_length_3033_cov_5.744795"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKM23_14240"
     CDS             complement(<1..181)
                     /locus_tag="PKM23_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92680.1"
                     /translation="MMKTTAAKIIFALLFCAGAAALFRPAGGDMARRRVVKYWFVAGV
                     KDNIPLCVQLFNAAQE"
     gene            complement(266..2890)
                     /gene="topA"
                     /locus_tag="PKM23_14245"
     CDS             complement(266..2890)
                     /gene="topA"
                     /locus_tag="PKM23_14245"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016971417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNY92681.1"
                     /translation="MPKILVIVESPAKAKTINKYIGGDYIVKSSVGHIRDLPPSAAQA
                     GEAVREEGGAKLSPGERKQRNLVRVMGIDPEGGWKAHYVILPGKTRVLRELKQAAAAA
                     EAIYLATDLDREGEAIAWHLREAIGGDPARYRRVTFSEITRSALQKAFGAPGQINMDR
                     VNAQQARRFLDRVVGYMVSPLLWQKIARGLSAGRVQSVAVRLIVEREREIRAFVPEEY
                     WELWADLEGKNPVRAQVVKRQGKTFKPVNKIETDLAMAELEKAVYEITERKDAPTLSR
                     PYPPFITTTMQQAASVRLGFGVKKTMLLAQRLYEAGHITYMRTDSFHLAPEALSAARH
                     LIQAEYGAKYLPEKPNFYASKAGAQEAHEAIRPTRAEAHPGSIPGLERDAERLYDLIW
                     RQFIASQMKPAEFDTTAITIAAGDYELRARGRVMRFDGWMRALPPAKREDETEMPDLR
                     VGDLLKLIRLDPSQHFTKPPARYSEASLVRELEKRGIGRPSTYASIITTIQERGYVKL
                     ISRRFYALKIGEIVTGRLIESFDDLMDYGFTAAMEEKLDAIAAGEVEWKKILDAFYGH
                     FKAQLEAAAGSMRRNEPVATDIPCAACGRPMVIRTAATGVFLSCSGYELPPKERCKST
                     VNLIPGDEAAPAKSVKESLEGEEEGDAEELLARRRCDKCGAAMDSWLLDARRKLYVCG
                     SSPDCDGWVIEEGTFKLKGYDGPSLVCDKCGAEMQLKSGRFGKYFGCSRYPECKNTRK
                     LLRGGQAAPPKSDPVPMPELKCKKSEAHFLLRDGAAGLFLAAHTFPKSRETASPKVAD
                     LARHRAELDQKFHYLADAPQSDPDGNSFEIRWKRKEGKQYLVGVTDAGEPSGWVAYFE
                     EGEWRSKREKVSAPRRRR"
BASE COUNT          487 a          935 c         1000 g          611 t
ORIGIN      
        1 tctcctgggc cgcattgaag agctggacgc agagggggat gttgtccttg acccccgcca
       61 caaaccagta ctttaccacc ctgcgccttg ccatgtcacc gccagcgggt cggaaaaggg
      121 ctgctgcacc ggcacagaac aaaagggcaa aaatgatttt ggcggctgtg gtcttcatca
      181 tgttttcggt ataagtaaaa aacaacctaa cccattattc ggatagatgc aagaaattta
      241 tcgccgccgg cggggcggtt tcctgctagc gccgccttct cggggcgctc actttttccc
      301 gcttgctccg ccactctccc tcctcgaaat aggccaccca gcccgagggc tcacccgcgt
      361 cggtcacccc gacgagatac tgtttgccct ccttgcgctt ccagcggatc tcgaagctgt
      421 ttccgtccgg gtcgctctgc ggggcatcgg cgagatagtg gaacttttga tcgagttcgg
      481 cgcggtggcg cgccaggtcg gccactttgg gtgacgcggt ctcacgcgat tttggaaaag
      541 tgtgggcggc gagaaagagc cccgcagccc cgtcgcgcag caggaaatgg gcctccgatt
      601 ttttgcattt aagttccggc atcggtacgg gatcactctt gggcggggcc gcctgccccc
      661 cgcgcagcag tttgcgggtg ttcttgcact cgggatagcg gctgcagccg aaatacttgc
      721 cgaagcggcc gctcttgagc tgcatctccg ccccgcactt gtcgcagacc agactcgggc
      781 cgtcatagcc cttaagcttg aaggtcccct cctcgatgac ccagccgtcg cagtcggggc
      841 tgctgccgca gacgtagagt ttgcggcggg cgtctagcag ccaactgtcc atcgccgctc
      901 cgcacttgtc gcagcggcgg cgggcgagca gttcctcggc atcgccctcc tcctcccctt
      961 ccagggactc cttcaccgat ttggccgggg cggcctcgtc gccggggatg aggttaacgg
     1021 tgctcttgca gcgctccttt ggcggcagct cgtagccgga gcagctcaga aagaccccgg
     1081 tcgcggcggt gcggatcacc atcggccggc cgcaggcggc gcaggggata tcggtcgcca
     1141 ccggctcgtt gcggcgcatg ctgccggccg cagcctccag ctgggccttg aagtggccgt
     1201 aaaaggcatc gaggattttt ttccattcca cctcaccggc ggcgatggcg tcaagctttt
     1261 cctccatggc cgcggtgaag ccataatcca tgaggtcgtc aaaactttcg atgagccggc
     1321 cggtgacgat ttcaccaatc ttgagggcgt agaagcgccg gctgatgagt ttgacgtagc
     1381 cgcgctcctg gatggtggtg atgattgagg cgtaggtgga ggggcgcccg atgccgcgct
     1441 tctccagttc gcgcaccagg ctggcctcgc tgtagcgggc cgggggcttg gtgaaatgct
     1501 gggaaggatc gaggcggatc agcttgagca ggtcgcccac cctgaggtcg ggcatctcgg
     1561 tctcgtcctc ccttttggcc gggggcaggg cacgcatcca gccgtcgaag cgcatcaccc
     1621 ggccgcgggc gcgcagttca tagtcgccgg ccgcgatggt gatggcggtg gtgtcgaatt
     1681 cggccggttt catctgggaa gcgatgaact ggcgccagat caggtcgtag aggcgctcag
     1741 cgtcgcgctc gagtccgggg atggagccgg gatgggcctc agcgcgggtc ggacggatgg
     1801 cctcgtgagc ctcctgggcc cccgccttgc tggcgtagaa attgggcttc tcgggcaggt
     1861 attttgctcc atactcggcc tggatcagat ggcgggcggc ggagagggcc tcaggggcga
     1921 gatgaaacga gtcggtgcgc atgtaggtga tgtggccagc ctcatagagg cgttgggcca
     1981 ggagcatggt ctttttgacc ccgaagccga ggcggacgct ggcggcctgc tgcatcgttg
     2041 tggtgatgaa ggggggatag ggacgggaga gggtcggggc gtccttgcgc tcggtgatct
     2101 cgtagaccgc cttttcgagt tcggccatag caagatcggt ttcgatcttg ttgaccggct
     2161 tgaaggtttt accctggcgc ttgaccacct gggcgcgcac cggattcttg ccctcgaggt
     2221 cagcccagag ctcccagtac tcctcgggga cgaaggcgcg gatctcacgc tcgcgctcga
     2281 cgatgaggcg cacggccacc gactggaccc gcccggccga gagcccgcgg gcgatctttt
     2341 gccagagcag gggagaaacc atatagccga ccacgcgatc gaggaagcgc cgcgcctgct
     2401 gggcgttgac ccggtccatg ttaatctgcc cgggcgcgcc gaaggccttt tgcagggcgc
     2461 tcctggtgat ctcactgaag gtcacccggc ggtagcgcgc cggatcgccg ccaatcgcct
     2521 cgcgcaggtg ccaggcgatc gcctctccct cgcggtcgag gtcggtggcg agatagatgg
     2581 cttcggccgc ggcggccgcc tgcttgagct cgcgcagcac ccgggttttt cccggcagga
     2641 tcacataatg ggccttccag ccgccctcgg gatcgatgcc catcacccgt accaggttgc
     2701 gctgcttacg ctcaccgggg ctcagttttg cgcctccctc ctccctgacc gcctcgccag
     2761 cctgggcggc gctcgggggc agatcgcgga tatggccgac ggaggatttg acgatgtaat
     2821 cgccgccgat atatttgttg atggttttcg ccttggcagg cgattcgacg atgacgagga
     2881 ttttaggcat gacgggtaga gtttcctttc aggctgaata ataatccctg accatcgctt
     2941 ccatcaccgc gctggtgcgc gcgccgctct ccccgctcga gaggggttcc gcctcgccac
     3001 gcagggcggc gacgatgttc tcgatgagga act
//
LOCUS       DAOPWU010000399         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_33525_length_3025_cov_4.872054, whole genome shotgun sequence.
ACCESSION   DAOPWU010000399 DAOPWU010000000
VERSION     DAOPWU010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33525_length_3025_cov_4.872054"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PKM23_14250"
     CDS             complement(<1..493)
                     /locus_tag="PKM23_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNY92682.1"
                     /translation="MKPCPKMQLAINLYHAGELDDGARLEVERHLERCPACREAAEQI
                     GEIARLAAEAPGLEDDRAAASARRSRLMAALTPGLPARQTTPLPARPMPLQPLYAALA
                     AMLLVAFGFLLGRFGGRQPAPDPTLQALLTGFQPVVAAGHTVQPELVNVQRVRYDPAS
                     GQFE"
     gene            complement(533..1102)
                     /locus_tag="PKM23_14255"
     CDS             complement(533..1102)
                     /locus_tag="PKM23_14255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNY92683.1"
                     /translation="MDPLESEWIIRARSGDLPAFGELVNRHDGYIMRLIRSMAPQEEA
                     EDLWQETFIKAFAGLTAFREESSFRTWLTRIAIRQCLDHRRQERWKRLVPLRAAPSGE
                     AESLLELPSPDPLPDQRAVRQEMWRHLQRALDNLPGGQRAAFVLKYVQGCTIHEVAEI
                     LGKAEGTIKSDLFRAMQRIRAQMQQLYAP"
     gene            1448..2296
                     /locus_tag="PKM23_14260"
     CDS             1448..2296
                     /locus_tag="PKM23_14260"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY92684.1"
                     /translation="MQPKSEAAAGKREGIDRQRAESFNRNAALYDAHRPGYPDDLYLE
                     LQRLTGVNENSVVIEIGAGSGIATEQMYHHWHATIYALEPGAALLAQAQKRVGHIPAI
                     HLVHSSFEDYTTAVPCDLIAAATSFHWVEPAVKFARIGKLLNPGGFLAAWWSNFSRND
                     TPVFDAIRAIYAQYHPEPELNDDLRGLQGQRVAERRSEFEQAPGLSFHAHREFHCTRS
                     FTADAYIGLLRTFSTNACHPEEHMALFYERIAALIHAQGNRLDVPFMVNLELARKDVS
                     PQRSGE"
     gene            2434..>3025
                     /locus_tag="PKM23_14265"
     CDS             2434..>3025
                     /locus_tag="PKM23_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNY92685.1"
                     /translation="MKLLVFGATGKTGRALLEQGLAQGHAVTAFVRNPAALEAQAGLT
                     IISGDVTDAAAVSRAVAGQEAVLSALGPRGGNYGVLPGGVQIIIAAMKQAGVKRLIHV
                     SSYGVGDSLAQMGWVSRKIVVPFFLRKALDEKEIEEGIIRASDLDWIIVRPGGLEDGA
                     RTGVYRCITDPLTKVGQPRIARADVADFILKNLVDEH"
BASE COUNT          599 a          860 c          969 g          597 t
ORIGIN      
        1 tctcgaattg tccgctggcc ggatcgtagc gcacacgctg aacattcacg agttccggct
       61 gtacggtgtg gccggctgcg accacgggtt gaaaaccggt cagcagcgcc tgcaacgtgg
      121 gatcgggagc gggctggcgg ccgccgaagc gtcccagcag aaagccgaag gccaccagca
      181 gcatcgctgc gagggcggcg taaagcggct gcaacggcat cggcctggcc gggagcggcg
      241 ttgtctgcct ggccggcagc cccggcgtaa gggcagccat cagccggctg cgccgggccg
      301 aggctgccgc ccggtcatcc tcgagaccgg gggcctcagc cgccagccgg gcgatctcgc
      361 cgatctgttc cgcagcctcg cggcaggcgg gacagcgctc gagatggcgc tcgacttcaa
      421 gccgggcgcc gtcatccagc tcgccggcat gatagaggtt gatggcgagc tgcattttgg
      481 ggcaaggttt catggctttt cccgatactg ttcttttgtg cgacgcaggc gcttacggcg
      541 catagagctg ctgcatctgc gccctgatgc gttgcatcgc gcggaagagg tcgcttttga
      601 tggtgccttc ggccttgccg agtatctccg caacttcatg aatggtgcat ccctgcacat
      661 acttgaggac aaaggccgcg cgttgaccgc cggggagatt atccagggcg cgctgcaggt
      721 ggcgccacat ctcctggcgc accgcccgct gatccggcag gggatcggga ctgggcagct
      781 cgaggaggct ctccgcttcc ccggaggggg cggcccgcag tggaaccagc cgtttccagc
      841 gctcctggcg gcggtgatcg agacactggc ggatagctat gcgggtcagc caggtccgga
      901 agctgctctc ctcgcgaaag gccgtcagcc cggcaaaggc cttgatgaag gtctcctgcc
      961 agagatcttc agcctcctcc tgcggcgcca tggaacggat gagccgcatg atgtagccat
     1021 catggcggtt taccagttca ccgaaggccg gcagatcgcc tgatcgggcg cgtatgatcc
     1081 attcgctttc gagagggtcc atgggattcc ctgattgtga cggtcaatgg attagacgca
     1141 gcgaacggga aaaaagttta cagggaaagg gagaaaaggc agaaaattgc cagaaaaagt
     1201 gaacgccccg caggatctac tcggagaggt gacagccggc tgcgtttgat gccgttgtta
     1261 tgctctgcga gctcttcaat ctctttactg aggatctctt cccggccaag tgcaattact
     1321 gaccttcgtg atgatttttg tctctgccct cgcgaacaat tctgttgaaa ttcaggatac
     1381 tcttgagtag attacttgat aaatcagtaa ttccataacg attgcattca gccccagagg
     1441 agcgctcgtg cagcccaaat ctgaagccgc agccggaaaa cgagagggaa ttgaccgcca
     1501 gcgggcggag tctttcaacc gcaatgcagc cctctatgac gcccaccggc cgggctatcc
     1561 ggacgatctc tatctcgaac tgcagcggct gacaggggtg aatgagaact cggtggtcat
     1621 cgagatcggc gccggcagcg gcatcgccac cgaacaaatg taccaccatt ggcatgcgac
     1681 catctatgcc ctcgaacccg gagcggcgct gttggcccag gcccaaaaac gggttggtca
     1741 catccccgcc atccatctgg tccacagttc gtttgaggat tatacgacgg cggttccttg
     1801 cgatctgatc gccgcggcga cctcgtttca ttgggtggaa ccggcggtca agttcgccag
     1861 gatcggcaag ctgctcaatc caggcggttt tttggcggcc tggtggagca atttttcgcg
     1921 gaatgatacg cctgtttttg atgccatccg cgcaatctac gcacaatatc acccggaacc
     1981 cgagctgaat gatgatcttc gcggcctcca ggggcaaaga gttgcggaga gaaggagcga
     2041 atttgagcaa gcgccaggcc tgagtttcca tgcccacagg gagtttcatt gtacgcgatc
     2101 tttcaccgcg gacgcttata tcgggctatt gcgcaccttc tcaaccaatg cctgccatcc
     2161 cgaggagcat atggcgttat tctacgagcg tatcgcagca ctcatccatg cccagggcaa
     2221 ccgtctcgat gtgcccttca tggtcaatct cgagctcgcc cggaaggatg tatcgcctca
     2281 gcgcagcggc gaataactct ccggcacgaa gagagaatag cgtagccgct acccctgccc
     2341 aaagtggtcg cgaaaggaaa aattgctggt aagggccgct tgcagccagg cgctgatgca
     2401 gggggggggt acattaaaaa ggagttaatc aggatgaagc ttttagtgtt cggtgctacc
     2461 ggcaaaacag ggcgtgcctt gctggaacag ggtctggccc agggccatgc ggtgacggcg
     2521 ttcgtgcgca acccggcggc gctggaggcg caggccggac tcacgataat ttcgggtgat
     2581 gtgaccgatg cggcggctgt cagccgtgct gtggccggcc aggaggcagt tctctcggcg
     2641 ctgggaccgc gcggtggcaa ctatggtgtg ctgccgggag gcgtgcagat catcatcgcg
     2701 gcgatgaagc aggcaggggt gaagcggctg attcatgttt cctcctacgg cgttggcgac
     2761 agtctggcgc aaatggggtg ggtgtcgcgc aagatcgttg tgcccttttt tttgcgcaag
     2821 gcgctggacg agaaggaaat cgaggagggg atcattcgcg cgagcgacct cgactggatc
     2881 atcgtccggc cgggtggact ggaggacggg gcaaggaccg gcgtctaccg ctgcatcacc
     2941 gacccgctga cgaaggtcgg ccagccgcgc attgcacgcg cggatgtggc ggattttatc
     3001 ctgaagaacc tggtggatga acact
//
LOCUS       DAOPWU010000400         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_34241_length_2981_cov_4.284689, whole genome shotgun sequence.
ACCESSION   DAOPWU010000400 DAOPWU010000000
VERSION     DAOPWU010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34241_length_2981_cov_4.284689"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2074)
                     /locus_tag="PKM23_14270"
     CDS             complement(<1..2074)
                     /locus_tag="PKM23_14270"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HNY92686.1"
                     /translation="MRKLLLSICILFTYNQAFSAASLQVINPSVPLYQFVTLSLDAGR
                     DVPNPFISITLSADISSPDGRLFHAEGFYNGGRSWLLRFMPDRTGSWSFSWQFGGESG
                     SGSFTCVSKQNSKLHGHIRIDANNSHKLRFEDGTPLHWIGGKYIDFDDPFYKTPEHAS
                     VPERMPKSQYLPLVHAYLQNIAAMGLNGVVLKLRVLPLNYDLQSMDLDFFASADQIMQ
                     WCMELGINVQLNFFDTWGKRKQGADISISNPPPSDLLLEPHDASTFVTKTRFFIRYAI
                     ARYAAYPNLLWELWNEAERMGASAQTASTLYATYFEEYDPYGIPISASEIHTAAYPLQ
                     VTSFHAGFKCAPSEWNWTHTRTDDPTLYKKWVNFGQYGYDYGRPILWNELYPYDGVDD
                     GGQYATNTAAHDWFRATFWGNLTAGCIGTSEFCWAPIDQVPNRVTEYHSHFAAFLSYL
                     KDFNALDPADSEVEATIGTATMCRKRGKEYVIYHFTQGRGSQTTLNVKLPPGYYYYQF
                     YDPKNGETASPRSMVNRSSEGWQSFATPLFNQDIVLYLIESSYYGVVTPVELAHLSGA
                     RDAAGVRLEWSTASETNNYGFEVARAGSGDGPFITAAFIPGHGTTAAAQDYNWVDTEA
                     GEGTLWYQLIQIDADGLRHAWPALRIAAVAPAAPVISTYPNPGRGMVRLRFHLDQPDR
                     VRLTIYNTL"
     gene            complement(2248..2661)
                     /gene="trxA"
                     /locus_tag="PKM23_14275"
     CDS             complement(2248..2661)
                     /gene="trxA"
                     /locus_tag="PKM23_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNY92687.1"
                     /translation="MATTTMSIDNFQNTIENNAIVLIDFWAGWCGPCKMFGPVFEKAS
                     EKHPDITFAKVDTEAQGDLASLFGIQSIPTLAIFRDGILLYREAGALPEAGLEELIGQ
                     VRKLDMDKVRADIENQKAEEAVDEHNCEGCAGCGH"
     gene            complement(2747..>2981)
                     /locus_tag="PKM23_14280"
     CDS             complement(2747..>2981)
                     /locus_tag="PKM23_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNY92688.1"
                     /translation="KPNPNYFLEVAHRLNVAPSSCLMVGNDYRDDMAAVAAGMETYMV
                     LDYALNSHLARHTPTYTGSLADLHDFLLQIRNS"
BASE COUNT          601 a          744 c         1034 g          602 t
ORIGIN      
        1 gcagggtgtt gtagatggtc aggcgcacgc ggtccggctg gtcaagatgg aaacggaggc
       61 gcaccatgcc gcggcccgga ttgggatagg tcgagatcac aggcgcggcc ggggcgacgg
      121 cggcgatgcg cagggcgggc caggcgtggc ggaggccatc ggcatcgatc tggatgagct
      181 gataccagag cgtgccctcc cccgcctcgg tgtcgaccca gttgtagtcc tgggcggcgg
      241 cggtcgtgcc gtgaccaggg atgaaggcgg cggtaataaa ggggccgtcg ccgctgccgg
      301 cgcgggcgac ctcgaaaccg tagttgttgg tctccgaggc tgtgctccac tccagccgca
      361 ccccggcggc atcgcgcgcg ccggagagat gagcgagttc gaccggggtc accacgccat
      421 aatagctgct ctcgatgaga tagaggacga tgtcctggtt gaagagcgga gtggcgaagg
      481 attgccagcc ttcgctgctg cggttgacca tgctgcgcgg gctggcggtc tcgccgtttt
      541 tggggtcgta gaactggtaa tagtaatagc ccggcgggag cttgacgttc agggtggtct
      601 ggctgcccct gccctgggtg aagtggtaga tgacgtattc cttgcctcgc ttccggcaca
      661 tcgtcgcggt gccgatggtg gcctcgacct cgctgtcggc aggatcgagg gcgttgaaat
      721 ccttgagata ggagaggaaa gcggcgaaat ggctgtgata ttcggtcacc cggttgggaa
      781 cctggtcgat gggcgcccag caaaactcgc tggtgccgat gcagccggcg gtgaggttgc
      841 cccagaaggt ggcgcggaac cagtcgtggg cggcggtgtt ggtggcgtac tggccgccat
      901 cgtcgacgcc gtcgtagggg taaagctcat tccagaggat gggacggcca tagtcatagc
      961 cgtactggcc aaaattcacc cattttttat agagcgtggg atcatcggtg cgggtatggg
     1021 tccagttcca ctctgaaggt gcgcatttga atcctgcatg aaaagaggtg acctgcagcg
     1081 gataggcggc ggtgtggatc tccgaggcgc tgatgggaat accgtaggga tcatactctt
     1141 cgaaataggt ggcgtaaaga gtggaagcgg tctgggcgga ggcgcccatg cgctcggcct
     1201 cgttccagag ctcccagagc aggttgggat aggcggcgta gcgcgcgatg gcgtaacgga
     1261 tgaaaaagcg ggtcttcgtc acaaacgtgg acgcatcgtg cggctcgagg agcaggtccg
     1321 agggcggggg attgctgatg gagatgtcag cgccctgctt gcgtttgccc caggtatcga
     1381 aaaagttgag ctggacgttg atgcccagct ccatgcacca ctgcatgatc tggtcggcgc
     1441 tggcgaaaaa atcgaggtcc atgctctgca ggtcatagtt gaggggcagg acgcgcagct
     1501 tgaggaccac gccgttgagc cccatggcgg cgatattctg caaataggcg tggaccaggg
     1561 ggaggtactg ggatttgggc atgcgttcgg ggacgctggc atgttccgga gtcttgtaaa
     1621 agggatcgtc aaagtcgata tacttgccgc cgatccagtg caggggggtg ccatcctcga
     1681 agcggagttt gtgcgaattg ttcgcatcga tacggatatg gccatgcagt ttgctgttct
     1741 gttttgatac acaagtgaac gaacccgagc ccgattcgcc gccgaattgc caggaaaagc
     1801 tccagctgcc ggtacggtcg ggcatgaaac ggagcagcca gctcctgccg ccgttgtaaa
     1861 agccttcggc atggaagagg cgcccgtccg gcgaactgat atctgcagat aaggttatgc
     1921 tgataaaggg gttggggaca tcccggcccg catccaggga aagggttacg aactgataaa
     1981 ggggcacgct gggattgatg acctggaggc tcgcggcgga gaaggcttga ttataagtaa
     2041 aaaggataca tatagatagc agaagttttc tcatggttgc ctcactgctg ttgcgatgag
     2101 cggatcgggc aggagttgtg ctcccggatg gaagtggcgg caaaaatgtt gcaaaaatcg
     2161 tgctatcgat cacatttttc ccagagcaaa aaaagggcgg gcagccggct gcccgccctt
     2221 ttcaggggat ttgcgatcga ggatggatca gtggccgcag ccggcgcagc cttcgcaatt
     2281 gtgttcgtca accgcctctt cggccttctg gttttcgatg tcggcgcgca ccttgtccat
     2341 atcgagcttg cgcacctggc cgatcagctc ctccagcccg gcctcgggca gggccccggc
     2401 ttcgcgatag agcagaatgc cgtcgcggaa gatggccagg gtggggatgg actggatgcc
     2461 gaagagcgaa gcgaggtcgc cctgggcctc ggtgtccacc ttggcgaagg tgatatcagg
     2521 atgcttctcg gaggcctttt caaagaccgg gccgaacatc ttgcaggggc cgcaccagcc
     2581 ggcccagaaa tcgatcagga cgatcgcatt gttttcgatg gtgttctgaa aattatcgat
     2641 ggacatggtg gtcgtagcca tggtaactcc ttatgatggg gatgctaaaa attggacggc
     2701 gattatgcct ttcaagagag ttggatgcag gcggcaccaa aaggattcag ctgtttctga
     2761 tctgcaagag aaaatcatga agatcggcga gcgagccggt gtaggtaggg gtgtgccggg
     2821 cgaggtggga gttgagggcg taatccagga ccatgtaggt ctccatgccc gcagcgaccg
     2881 cggccatgtc gtcccgataa tcgttgccca ccatcaggca gctcgagggg gccacgttga
     2941 ggcggtgggc gacctcgaga aaataattcg gattgggttt g
//
LOCUS       DAOPWU010000401         2973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_34373_length_2973_cov_3.577450, whole genome shotgun sequence.
ACCESSION   DAOPWU010000401 DAOPWU010000000
VERSION     DAOPWU010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34373_length_2973_cov_3.577450"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKM23_14285"
     CDS             complement(<1..223)
                     /locus_tag="PKM23_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92689.1"
                     /translation="MKRIIALSLLAALLAASGCKDKSPTGPDPADKATKSAKRGIAFN
                     LASPADFDALSKGVSWWYNWAITPSTSAPA"
     gene            complement(228..536)
                     /locus_tag="PKM23_14290"
     CDS             complement(228..536)
                     /locus_tag="PKM23_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNY92690.1"
                     /translation="MKEPDPIEIPIDGALDLHTFAPRDVKELVPDYLTACRERGILEV
                     RIIHGKGIGALRETVHAMLRRLPWVLHFQLAGDGRGGWGATIATLAPLNNSDMIDDKE
                     "
     gene            complement(546..896)
                     /locus_tag="PKM23_14295"
     CDS             complement(546..896)
                     /locus_tag="PKM23_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNY92691.1"
                     /translation="MEWLDGIAGAIVVCDTQGIILYLNRQAEINFAGEGGAALIGSNL
                     LDCHPEPSRSKLRELLAEQKTNVYTIEKRGIKKLIWQKPWYRDGVFAGLVEFSLEIPR
                     EMPHFVRSAAAEPV"
     gene            1321..1935
                     /locus_tag="PKM23_14300"
     CDS             1321..1935
                     /locus_tag="PKM23_14300"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP family protein"
                     /protein_id="HNY92692.1"
                     /translation="MKKIITAAVLIALLIILGRGLRNNSGYDAEPASRMTANENAAPS
                     TVQPAATEGGGSGSSLNGEEADLLLTLHNRARAAAGTRPLKWSEELARYAQEWADHLA
                     ASATFDHRPESGRWKQLYGENLYMGTAAYYGLERAVDAWVEEKSRYNGGPISEANVAA
                     AGHYTQVVWKNTRAMGCGKALRDGQLIVVCNYDPAGNVEGERPW"
     gene            complement(2020..2472)
                     /locus_tag="PKM23_14305"
     CDS             complement(2020..2472)
                     /locus_tag="PKM23_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92693.1"
                     /translation="MIAYRNSQTGWLIILLLAAGFIYTLSTLSLDAVTTGQYIVLLVL
                     IVAGVLFYKLNVVVERGVIVVSFGQGMISKSIRARDLAGCCVVKVPWYYGFGIRLTPS
                     GWLYNVSGSNAVELTYRSGKKFIIGSNEADVLCETIQDEISKYATPQS"
BASE COUNT          608 a          846 c          925 g          594 t
ORIGIN      
        1 ccgccggggc gctggtgctc ggggtgatcg cccagttata ccaccagctc acgcccttcg
       61 agagggcgtc aaaatcggca ggcgaggcga ggttgaaggc gatgccgcgc ttggcgctct
      121 tggttgcctt atctgccgga tcgggtccgg tcggtgattt gtccttgcag ccgcttgccg
      181 caagcagggc ggcgagcagg ctcagggcga tgatccgctt catgctctta ctccttgtcg
      241 tcgatcatgt cagagttgtt caacggggcc agcgtggcga tggtcgcgcc ccagccgccg
      301 cgtccatcgc cggcgagctg aaaatggagc acccagggca gccgcctcag catggcatgg
      361 acggtttcgc gcagcgcccc gatccccttg ccgtggatga tgcgcacctc aaggatcccc
      421 cgttcgcggc aggcggtgag atagtcgggg acgagttcct tgacgtcgcg gggcgcaaag
      481 gtgtgcagat cgagcgcccc gtcgatgggg atttcaatgg gatcgggttc tttcaaggcc
      541 tctcctcaaa ccggctcagc tgcggcgctg cggacaaaat ggggcatctc ccgggggatc
      601 tcaagcgaaa attcgaccag ccccgcaaat acgccgtcgc ggtaccaggg cttctgccag
      661 atcagtttct tgatgccccg tttctcgatg gtgtagacat tggttttttg ttcggcgagc
      721 agctcgcgga gcttgctgcg cgagggctcg ggatggcagt cgagcaggtt gctgccgatc
      781 agagccgcgc ccccctcgcc ggcaaagttg atctccgcct gccggttcag atagaggatg
      841 attccctggg tgtcgcagac cacgatcgcc ccggcgattc cgtcaagcca ttccatcgct
      901 ttctttcacc tccctttgat acggttaaga tgcactggac tctaaggcta atattaggca
      961 ttcggcgggt gaaagtcaat ggatttgggg gaaggggagg ttatccccgc aatctggtcc
     1021 cacccggcag cttgcgccgc gttcccctcc gaaaatggag gtagccagtt gtctcattgt
     1081 ttgcccccgc tcatcgatgg tcgcgtcgga tggcacccgc ttttggcgct agcccgggct
     1141 ggctgcgcac agtccgcaga aaatgattgc attaaaagac tgaaaagagt aacatgggag
     1201 gagaatggcg tgcccgggag gagggatggt ccacacgcga gcctatcgcc tgcgccatcc
     1261 tgcgctctgt ctccgccctc ccggcctttc aagtctgatc attaactgga agaggacgta
     1321 atgaagaaaa tcatcacggc ggcggtttta atcgcacttt tgatcatcct gggcagggga
     1381 ttgcggaata actccgggta cgacgctgag ccggcttcca ggatgacggc gaacgagaac
     1441 gctgctccat ccactgtcca gcccgcggca acggagggtg gcgggagcgg ctcctcactc
     1501 aatggtgagg aggccgatct cctcctgacc ctgcacaacc gggcgcgggc cgcggcaggc
     1561 acccggccct tgaaatggtc ggaagagctg gcgcgctatg cgcaggagtg ggccgaccac
     1621 ctcgccgcct ccgccacctt tgatcaccgt ccggagagcg gccgctggaa gcagctttac
     1681 ggcgagaatc tctatatggg gacggcggct tattacgggc tggagcgggc ggtggatgcc
     1741 tgggtcgagg agaagagccg ctacaacggc ggtcccatca gcgaagccaa tgttgcggcg
     1801 gccgggcact atacccaagt ggtctggaaa aacacccggg cgatgggttg cggcaaagcc
     1861 ctgcgcgacg gccagctcat cgtcgtctgc aactatgatc ccgccggcaa tgtggaaggc
     1921 gagcggccct ggtagcgagt cgtcctgatc agctcggcgg agagccgggc gccctgagat
     1981 cgctgcgcca tggcccggtg aggggcgcgc aagtcgggat cagctctgcg gggtggcgta
     2041 tttgctgatc tcatcctgaa tggtctcgca gagcacatcc gcctcgttgc tgccgatgat
     2101 gaatttttta cccgatctgt aggtgagctc gacggcgttt gaaccggaga cattatagag
     2161 ccagcctgag ggcgtcagcc ggatgccaaa gccgtaatac cacggcacct tgaccacgca
     2221 gcagccggcg aggtcgcgcg cccggatgct tttgctgatc atcccctggc cgaacgagac
     2281 cacgataacc ccgcgctcga caacgacatt gagcttgtaa aagagcacgc cggcgacgat
     2341 gaggaccagc agcacgatat actgcccggt ggtgacggca tcgagcgaga gggtggagag
     2401 ggtgtagatg aatcccgccg ccagcagcaa aatgatcagc cagccggtct gcgagttgcg
     2461 ataggcgatc atgatgatcc tccttcaggt catcgggttc aggtcaggat tttgaatgtg
     2521 cggcatccct ataatctaat atacccaatt catggatctg tttcaacctg aaaacggaac
     2581 aggatacccc cgaaatctgg tccccaccga caccttgtgc cggatatatt aaaatcgggt
     2641 cagcctggat cagggcctca ggacagagtt cgccctcatc gaggagctga cggacatggg
     2701 cgtgaatgcg cgaatccctg gtctggataa aacgctcgac gtaatcccgg taatccctga
     2761 tcaaccgttt gcgaaaatcc aacacatcca taagtgagcc tttgcctgga gagattcact
     2821 gcctgtgcaa gaatgtatat ggggatctct atgctgagtt ggagtgatta gctggcaggc
     2881 tctgaaaccc atcaccacgt tgcttgaagg taaattttgc aggatattgg tcaaagtcaa
     2941 gggaaacagg gagacaagac aataaaggag ctt
//
LOCUS       DAOPWU010000402         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_34566_length_2962_cov_3.303062, whole genome shotgun sequence.
ACCESSION   DAOPWU010000402 DAOPWU010000000
VERSION     DAOPWU010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34566_length_2962_cov_3.303062"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /gene="rsxC"
                     /locus_tag="PKM23_14310"
     CDS             <1..1020
                     /gene="rsxC"
                     /locus_tag="PKM23_14310"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNY92694.1"
                     /translation="EEAWNPDIALQEDYLALERAEMISRIRAAGLAGMGGAAFPTHVK
                     LSPPADKPVDTVLLNGAECEPYLTSDHRLMLEKPAEILLGLQIIVKLLGAKQAAIGIE
                     NNKPDAVKLLRKKASELNLPYEIIPLKVKYPQGAEKQLIRATTGRVVPAGGLPMEAGC
                     VVQNVGTAAAVYDAVAWRRPLVERIVTVTGKGIKEPKNLLVRIGTPYSFVLEHCGGLT
                     EEAGKLIMGGPMMGLAQSSLEVPVIKGTSGLLVLDAREGKKHEERVCISCAHCVDACP
                     MGLVPKQIVALVRNGRIAEAGENHALDCIECGSCAYVCPAHINLVHWLRYGKSEIIRS
                     RKKAG"
     gene            1196..2233
                     /locus_tag="PKM23_14315"
     CDS             1196..2233
                     /locus_tag="PKM23_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNY92695.1"
                     /translation="MERSFYVSSSPHITDRESIPRIMYTVTLSLLPAAGYSVWLFGLR
                     ALVIMLLSMAACVATEALVQKLMKRPVTVSDGSALLTGLLLSFNVSAQVPLWLPVVGS
                     VFAIAVGKQVFGGLGYNPMNPALLGRAFLLASWPTHMTVFSGAPPAGGTVSGIDVITS
                     ATPLNVLKQAREVLANAPAYPVEKVNQAHEAVGQLYDSIDKIFLGQIGGCIGETSALL
                     LILGGLFLLYKRYIGWEIPVSYIGSVALLAWIFGGTEGAFSGNILFHIFSGGLMLGAF
                     YMATDMVTSPITSRGRLIFGLGCGLITMVIRLVGGYPEGVSYSILLMNLLVPVIDRSI
                     RPKVFGGGAKS"
     gene            2230..2802
                     /locus_tag="PKM23_14320"
     CDS             2230..2802
                     /locus_tag="PKM23_14320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNY92696.1"
                     /translation="MKEIVRFGLILMIVSVVAATALAAVYGVTNPRILAQKEAQLQAA
                     LAAALPGSDPASLKTVRENGEVLYYAAFAPGDTSKPMGYAFLAMGAGYSSTIETIVGV
                     DTLGRILGMKVLYQSETPGLGTKLQEVKYGDKEPWFPRQYLGKPAANVAVDKDGGEIR
                     SITGATISSRAVTRAINEGYARLRTKLGTN"
     gene            2810..>2962
                     /locus_tag="PKM23_14325"
     CDS             2810..>2962
                     /locus_tag="PKM23_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNY92697.1"
                     /translation="MAFISDLTRGIYRENPVLKQALGLCPTLAVSSSVSNGIGMGLAA
                     TFVLIGS"
BASE COUNT          540 a         1008 c          868 g          546 t
ORIGIN      
        1 gaggaggcct ggaatcccga catcgccttg caggaggatt acctcgccct cgagcgcgcc
       61 gagatgatca gccgcatccg cgccgccggg ctggccggca tgggcggggc cgcctttccg
      121 acccacgtca agctctcgcc gccggccgac aagccggtcg ataccgtcct gctcaacggg
      181 gccgagtgcg agccctatct gacctccgac caccgcctga tgctggaaaa gcccgccgaa
      241 atcctcctcg gcctgcagat catcgtcaaa ctgctcggcg ccaaacaggc cgcgatcggc
      301 atcgagaaca acaaacccga cgccgtcaag ctgctgagga aaaaggcgtc cgagctcaat
      361 ctcccctacg aaatcatccc gctcaaggtc aaataccccc agggcgccga aaagcagctc
      421 atccgggcga ccacagggcg cgtcgtcccc gccggcggat tgcccatgga ggccggctgc
      481 gtggtgcaga atgtcggcac cgccgctgcg gtctatgacg cagtggcctg gcggcgcccc
      541 ctggtggaga ggatcgtcac ggtcactggc aagggcatca aggagcccaa aaacctcctc
      601 gtccgcatcg gcacccccta cagctttgtg ctcgagcact gcggcggcct cacggaagag
      661 gcgggcaagc tcatcatggg cgggccgatg atggggctgg cccagtcctc cctcgaggtg
      721 ccggtgatca agggcacctc cggcctgctt gtcctcgacg ccagggaggg gaagaagcac
      781 gaggagcggg tctgtatctc ctgcgcccac tgcgtcgacg cctgtccgat gggactggtg
      841 ccgaagcaga ttgtcgccct ggtgcgcaac ggccgcatcg ccgaggccgg cgaaaaccat
      901 gccctcgact gcatcgagtg cggttcctgc gcctatgtct gcccggcaca catcaatctg
      961 gtccactggc tgcgctatgg caagagcgaa atcatccgca gccgcaaaaa agcggggtga
     1021 tccctttccc gcagtgagcc tgtaaatttc gcgccaagac gcaaagagaa taaagaaaat
     1081 tttctcacga agacacgaag gcaccaagag aagcataata gtatttttcc tttgtggctt
     1141 tgtggcttgg tgagacaatg ggtttctaaa aaatacctaa tcaacggatt cctcaatgga
     1201 acgctccttc tacgtcagca gttcgcccca catcaccgac cgggagtcga tcccgcggat
     1261 catgtacacc gtcacgctct cgctgttgcc cgccgccggc tattcggtct ggctcttcgg
     1321 cctgcgcgcc ctggtgatca tgctgctgag catggccgcc tgtgtcgcca ccgaggcgct
     1381 cgtgcaaaag ctgatgaaac ggccggtcac ggtcagcgac ggcagcgccc tgctcaccgg
     1441 actcctgctc tccttcaatg tctcggcgca ggtgcccctc tggctgccgg tggtcggctc
     1501 ggtgtttgcg attgcggtcg gcaaacaggt cttcggcggg cttggctaca acccgatgaa
     1561 cccggccctg ctcgggcgtg cctttctgct cgcctcctgg ccgacccaca tgaccgtctt
     1621 cagcggcgcg cccccggcgg gcggcacggt ctccggcatc gacgtcatca ccagcgccac
     1681 ccccctcaac gtcctcaagc aggcccgcga ggtgctcgcc aacgctccgg cctatccggt
     1741 cgaaaaggtc aaccaggccc acgaggcggt cggccagctc tatgacagca tcgacaagat
     1801 ctttctcggc cagatcggcg gctgcatcgg cgagacctcc gcgctgttgc tcattctcgg
     1861 cggcctcttc ctgctctaca agcgctatat cggctgggag attccggtca gctatatcgg
     1921 ctcggtcgcc ctgctcgcct ggatcttcgg cggcaccgag ggggccttca gcggcaatat
     1981 cctctttcac atcttttccg gcggcctcat gctcggggcc ttttacatgg ccaccgacat
     2041 ggtcacctcg ccgatcacct ccaggggccg cctcatcttc ggcctcggct gcggcctcat
     2101 caccatggtc atccgcctgg tcggcggcta tccggagggg gtctcctact cgattctgct
     2161 tatgaacctg ctcgtcccgg tgatcgaccg ttccatccgg ccgaaagtct tcggcggagg
     2221 tgcgaagtca tgaaagagat cgtccgtttc ggcctcatcc tgatgatcgt ttcggtcgtc
     2281 gccgccaccg ccctggcagc agtttacggc gtcaccaatc cccgcatcct cgcccagaag
     2341 gaggcccagc tccaggcggc cctggcggcc gccctgcccg gcagcgatcc ggccagcctc
     2401 aagaccgtgc gcgagaatgg cgaggtgctt tactatgccg ccttcgcccc gggcgatacc
     2461 agcaagccca tgggctacgc ctttctcgcc atgggggcgg gctactccag caccatcgaa
     2521 accatcgtcg gcgtcgacac cctgggccgc attctcggta tgaaggtgct ctaccagagc
     2581 gagacccccg gcctcggcac caagctgcag gaggtcaaat acggcgacaa ggaaccctgg
     2641 ttcccgcgcc agtatctcgg caagccggcg gcaaacgtgg ccgtcgacaa ggacgggggg
     2701 gagatccgat ccatcaccgg ggcgaccatc tcgtcgcggg ctgtgacccg cgccatcaat
     2761 gaaggctatg caaggctgcg caccaaactg ggtacaaact gaggctttta tggcatttat
     2821 cagcgattta acccgcggca tttaccggga gaatcccgta ctcaaacagg ccctgggatt
     2881 gtgcccgacc ctggcggtct ccagctcggt ttccaacggc atcggcatgg ggttggcagc
     2941 cacgtttgtt ctcataggct cc
//
LOCUS       DAOPWU010000403         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_34582_length_2961_cov_4.457330, whole genome shotgun sequence.
ACCESSION   DAOPWU010000403 DAOPWU010000000
VERSION     DAOPWU010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34582_length_2961_cov_4.457330"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..781
                     /locus_tag="PKM23_14330"
     CDS             137..781
                     /locus_tag="PKM23_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNY92698.1"
                     /translation="MQTVIDIVLLLVLLSDLYLLSLTRLGASIRVMAFQGVLLALLPL
                     LSAGGILSLHGLIIAVSSLAIKAILIPRFLSHAAREVHTGLAIDSHIPYGAALLTGMG
                     MVLVSFWVARKFALPVTVTSRLWIPVSLAGVMIGLLMLIIRKKAISQVIGYLVLENGI
                     YILGLSLAAQMPFLVELGVLLDVFAGVFIMGIIMNHIKNYFDDLDVDNLTILKD"
     gene            784..2229
                     /locus_tag="PKM23_14335"
     CDS             784..2229
                     /locus_tag="PKM23_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006008034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNY92699.1"
                     /translation="MIAAVIVLPIFFSAAAWLVRNSRLRRRLWLICSLIHVGMVASLA
                     LQPAQPILGGWLAVDALGLLFLGIVSLLFFFSAAYGQGWLAQAHTEVVKGVRDPERIF
                     IVCILLFLSTMSLVVLSHHLGLLWVAVEATTLASAPLIFFHRTVHSLEATWKYLLICS
                     VGIALALFGTFFLGAAASAGGREGSLLLDGLLRLGPQLDVRLTRIAFIFLLVGYGTKM
                     GLAPMHTWLPDAHSEAPSVVSSLLSGVLLNCAFLAILRAYQICQAAGDAAFARNILLI
                     FGLVSMLLAAVFILNQPDYKRLLAYSSVEHMGILAFGIGLGPAGYYFALFHALNHSLT
                     KSGLFLTAGNILSLFKTKAIGEVKGLGASHPWVGWAWVTGFLAISGTPLFGLFISEFN
                     LLGVAVLGNRLIVAALYLFLLTAVFIGMAAAFFPMVQGRPQQGAREKLPAALQLLPLL
                     LFLLTFLLGVHIPAGLDELLRLGAAALGGGQ"
     gene            2226..>2961
                     /locus_tag="PKM23_14340"
     CDS             2226..>2961
                     /locus_tag="PKM23_14340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNY92700.1"
                     /translation="MNRRAFVPFHNGEAVDWSTLAALPFNSFRDAVLTAAAGGQRLAS
                     FFGLRQGRGKLRLIAVLADDQNGVLYGLRTQVEASFPALTPDCPQAHLYEREIFEQFG
                     VQPEGHPWLKPVRFEPTAGSEVITPPEIGTADFFAMHGDEIHVVAVGPVHAGVIEPGH
                     FRFQCHGEKVYHLEISLGYQHRGIEKALAGGPYSRTLYQMEAAAGDTTIGHALAYATA
                     LEALNGTRVPARAQAIRGIALELERLA"
BASE COUNT          406 a         1005 c          886 g          664 t
ORIGIN      
        1 cgttcttttc atcatagcca ccgccatcgg catcgttgaa tcggtgatgg cgcgtctgcg
       61 gctggggcag gtgccgcaga tgctgatggg ggccggcgcc ctcggcctgc tcagttacat
      121 cattctggga taacgcatgc aaaccgtgat tgatatcgtc ctgctgctgg tgctcctgag
      181 tgatctttac ctcctcagcc tgacccgtct gggggcctcc atccgggtga tggcctttca
      241 gggggtgctg ctagccctgc tgcccctgct ttcagccggg gggatcctct ccctgcacgg
      301 gctgatcatc gctgtgagca gcctggcgat caaggccatc ctcatccccc gctttctctc
      361 ccacgccgcc cgcgaggtgc acaccggcct ggcgatcgac tcccatatcc cttacggtgc
      421 ggccctgctc accggcatgg ggatggtgct ggtctccttc tgggtggcgc gcaagttcgc
      481 cctgcctgtg acggtcacct cgcggctgtg gattccggtc tccctggctg gggtgatgat
      541 cggcctgctc atgctcatca tccgcaaaaa ggcgatctcg caggtgatcg gctatctggt
      601 gctggagaat ggcatctata tcctcggtct ctccctggcg gcccaaatgc ccttcctggt
      661 cgagctcggg gtgctgctcg acgtcttcgc cggggtattc atcatgggca tcatcatgaa
      721 tcacatcaaa aactatttcg atgatctcga tgtggacaat ctgaccattc ttaaggatta
      781 gcagtgatcg cggcggtaat cgtacttccc attttcttca gcgcggcggc ctggctggtc
      841 cggaacagcc gtctgcgccg gcgcctgtgg ctgatctgct ccctgatcca tgtcgggatg
      901 gtggccagcc tggcgctgca gcccgcgcag ccgatcctgg gcggctggct ggcggtcgat
      961 gccctcggcc tgctcttcct cggcatcgtc tcgctcctct ttttcttcag cgccgcctac
     1021 gggcagggct ggctcgcgca ggcgcacacg gaggtggtga agggagtccg ggatccggag
     1081 cggatcttca tcgtctgcat actgctcttt ctcagcacca tgtccctggt tgtgctctcg
     1141 caccatctcg gcctgttgtg ggtggccgtc gaggcgacca ccctggccag cgcgccgctg
     1201 atctttttcc accgcaccgt ccattccctc gaggccacct ggaaatacct gctcatctgc
     1261 tcggttggca tcgccctcgc cctcttcggc actttctttc tcggagccgc cgcctcggcc
     1321 ggcggacggg agggatccct gctgctcgac ggactgctgc gcctggggcc gcagctggat
     1381 gtccgcctga cgcgcatcgc cttcatcttt cttctggtcg gctacggcac caagatgggc
     1441 ctcgccccga tgcacacctg gctgcccgac gcccacagcg aggccccctc ggtggtctcc
     1501 tcgctcctct ccggtgtgct gctcaactgc gcctttctcg ccatcctgcg cgcctatcag
     1561 atctgccagg ccgcgggcga cgccgccttt gcccgcaaca tcctgcttat cttcggactg
     1621 gtgtcgatgc tgctggcggc ggtttttatt ctcaaccagc ctgactacaa gcgcctgctc
     1681 gcctattcga gcgtcgagca tatgggcatt ctcgccttcg gcatcggact ggggccggcg
     1741 ggttattatt tcgccctctt tcacgccctc aaccattccc tcaccaaaag cgggctcttt
     1801 ctgaccgccg gcaacatcct ctcccttttc aagaccaagg cgatcggcga ggtgaaggga
     1861 ctgggtgcaa gccatccctg ggtcggctgg gcctgggtga ccggttttct cgccatttcc
     1921 gggacgcccc tgttcggcct cttcatcagc gagttcaacc tgctcggggt tgcggtgctc
     1981 gggaaccgcc tcatcgttgc cgcgctctac ctcttcctgc tcacggccgt tttcatcggc
     2041 atggccgcgg cctttttccc gatggtgcag ggccggcctc agcagggagc ccgggaaaaa
     2101 ctgccggccg ccctgcagct gctgcccctc ctgctcttcc tgctcacttt tctcctcggc
     2161 gtgcacatcc ccgccgggct ggacgaactg ctcaggctgg gcgccgccgc cctgggaggc
     2221 ggacaatgaa ccggcgtgcg ttcgtcccct tccacaacgg cgaggcggtc gactggtcca
     2281 ccctggccgc tctgcccttt aacagttttc gcgatgccgt gctgaccgcg gctgccggcg
     2341 ggcagcggct ggcatcgttt ttcggtctgc gtcagggccg cggcaagctc cgcctcatcg
     2401 ctgtgctcgc cgacgatcag aacggggtgc tctatggact gcgcacgcag gtggaggcct
     2461 cctttcccgc cttgacgcct gattgtcccc aggcccatct ctatgagcgc gagattttcg
     2521 agcaattcgg cgtgcagccg gaggggcatc cctggctcaa accggtgcga tttgagccga
     2581 ccgctggatc ggaagtcata acgccccctg agatcggtac ggctgatttt tttgccatgc
     2641 acggggatga aatccatgtg gtggcggtcg gcccggttca tgccggggtg atcgagccgg
     2701 gccatttccg ctttcagtgc cacggcgaga aggtctatca tctcgagatc tccctgggct
     2761 atcagcaccg cggcatcgaa aaggcgctgg ccggagggcc gtattcgcgc accctttatc
     2821 agatggaggc ggcggccggc gacaccacca tcggccatgc cctggcctat gcgacggccc
     2881 tggaagctct caacggcacg cgggtgccgg ctcgggcgca ggccatccgc ggcatcgccc
     2941 tcgagctgga gcggctggcc a
//
LOCUS       DAOPWU010000404         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_34676_length_2955_cov_4.268276, whole genome shotgun sequence.
ACCESSION   DAOPWU010000404 DAOPWU010000000
VERSION     DAOPWU010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34676_length_2955_cov_4.268276"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKM23_14345"
     CDS             complement(<1..658)
                     /locus_tag="PKM23_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92701.1"
                     /translation="MKRLMICAAALALAANSFAQSRGIPAVEPLTAWQVADVATDYRP
                     APPAPGIAPAAGLVKGSTADYVLLDQITIADYVMPGERWAVAFDPARRSLRLHTPAAA
                     ARLNEAAIAAIERAPDWLEQDLRLTFTLLTPEFQTLWANGILEAEEPCIDEVAFAVAH
                     SSPQYLMSGYGSPGLFLENARSIYANDAVLDYVEVVDHGTSAGDPDYYTTTRYKTLRS
                     G"
     gene            complement(758..2785)
                     /locus_tag="PKM23_14350"
     CDS             complement(758..2785)
                     /locus_tag="PKM23_14350"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNY92702.1"
                     /translation="MKKILLVILLLAASAPAQERYTADWKSLDARPTPQWYRDAKFGI
                     FIHWGLYSVPAFTCKGGYAEWYWNGIADTSTFLHKWHHRTYGDDFQYQDFAPLFKAEL
                     YRPDKWAELFERAGARYVVLTSKHHDGFCLWPNTQSWNWNSVDVGPHRDLLGDLTTAV
                     RARGLRMGFYYSLLEWFNPLYRSDIHRYVDEHMLPQLKEAVSLYQPSIIFSDGEWDHD
                     SKVLRSEEFLAWLYNDSPAPRDVVVNDRWGGETRFNHGGYFSTEYEAATSERSAAFQA
                     RGWEECRGMGRSFGFNRNEELEDYQTAESLIHMLVEIVSRGGNLLLNIGPAADGTIPV
                     IMQERLLQMGEWLRVNGEAIYGSRLNRTTGEGDSVRFTRSGDGRRLYAICLRHPGAQL
                     RLRTVEAMPGSAVRLLGLDRDLDWRQEEGELVVNLPAGTIHRMPRDHAWVFRIQARPY
                     VERPLIQAASFISIDKPLRVTLAAEPADAQIRYTLDGAEPTPASPLCTAPLRIDRSGL
                     LRVRAFRDGYAPSLTVEQQISVLSSSENGLTCRYYEGEWEQLPDFDALKPVRELRAWD
                     FSFAPVIKREENFSVVFDGFLRIDRPGLYTFYTRSDDGSRLWIDGRLVVDNDGLHGAR
                     EAQGKIELAPGRHALRVGFLEKGGQELLQVSFEGPELLKQPLPPSLLYYKR"
BASE COUNT          531 a          857 c         1035 g          532 t
ORIGIN      
        1 tgccgctgcg cagggtcttg tagcgggtgg tggtataata atcggggtcg ccggccgagg
       61 tgccgtggtc gaccacctcg acataatcca gcacggcgtc gttggcgtag atcgagcggg
      121 cattctcgag gaaaagcccg ggcgagccgt agccggacat caggtactgg ggtgaggagt
      181 gggcgacggc gaaggcgact tcatcgatgc agggctcctc cgcctcgagg atgccgttgg
      241 cccagagggt ctgaaattcc ggggtgagca gggtaaaagt gaggcgcaga tcctgctcca
      301 gccagtccgg ggcgcgctca atggcggcaa tggctgcctc gttcaaccgc gccgcggcag
      361 ccggcgtgtg cagcctcagg ctgcggcggg cggggtcaaa ggcaaccgcc cagcgctcac
      421 ccggcatgac atagtcagcg atggtgattt ggtcgagcag cacatagtcg gctgtgctgc
      481 ccttgacaag tcctgccgcg ggggcgattc cgggcgcagg gggagccggc cggtagtcgg
      541 tggcgacatc ggccacctgc caggcggtga ggggctcgac tgcggggatg ccgcggctct
      601 gggcgaagct gttcgccgcc agggcgaggg cggcggcgca tatcatcaaa cgtttcatat
      661 ctctcctgtc actgaagggg catctgcgga gtgcggggca gcatgcggtt cgtcgtcatc
      721 acggccggac ggcttcggcg ctataggcga tgatctccta ccttttgtaa taaagcagcg
      781 agggcggcag gggctgtttc aacagctccg gcccctcgaa ggagacctgc agcagttcct
      841 ggcctccctt ctcgagaaag cccacgcgca gggcatggcg gccgggcgcc agttcgatct
      901 tgccctgggc ctcgcgggcg ccgtgcaggc cgtcgttgtc gacgacgaga cggccgtcaa
      961 tccagaggcg cgagccgtcg tcggagcggg tgtaaaaggt gtagagcccg gggcggtcga
     1021 tgcggagaaa accgtcaaag acaacggaga agttctcctc gcgcttgatg acgggggcga
     1081 aagagaaatc ccaggcccga agttcgcgca ccggcttgag cgcgtcgaaa tcgggtaact
     1141 gctcccactc cccctcgtaa tagcggcagg tgaggccgtt ctcgctgctg ctgaggacac
     1201 tgatctgctg ctccacggtc aggctggggg catagccgtc gcggaaggcg cgcacgcgca
     1261 gcaggccgct gcggtcgatg cgcaggggtg cagtgcagag ggggctcgcc ggggtcggct
     1321 ctgcaccatc cagggtgtag cggatttgcg catcggccgg ttcggcggcg agggtgacgc
     1381 gcaggggctt gtcgatgctg ataaaagagg cggcctgaat gagcggccgt tcgacgtagg
     1441 ggcgggcctg gatccggaaa acccaggcat gatcgcgggg catgcgatgg atcgtcccgg
     1501 ccggcagatt gacgaccagc tcgccctcct cctgccgcca atccagatcg cggtcgagcc
     1561 ccagcagccg caccgcgctg ccgggcatgg cctcgacggt gcgcaggcgc agctgcgcgc
     1621 cgggatggcg caggcaaatg gcatagagcc ggcggccgtc gccgctgcgg gtaaagcgca
     1681 ccgaatcccc ctcgccggtg gtgcggttga ggcggctgcc atagatggct tcgccgttga
     1741 ccctgagcca ttcgcccatc tgcagcagcc gctcctgcat gatcaccgga atagtgccgt
     1801 cggcggccgg gccgatgttg agcaggagat tgccgccgcg gctgacgatc tcgaccagca
     1861 tgtggatgag ggattccgcg gtctgatagt cctccaactc ctcgttgcgg ttaaaaccga
     1921 aggagcggcc catgccgcgg cactcctccc agccacgcgc ctggaaagca gccgagcgct
     1981 cgctggtcgc cgcctcatat tccgtcgaga aataaccgcc gtggttgaaa cgggtctcgc
     2041 cgccccagcg gtcattgacc accacgtccc tgggggccgg ggagtcgttg tagagccagg
     2101 cgagaaactc ctcgctgcgc agcaccttgc tgtcgtgatc ccactccccg tcggagaaga
     2161 tgatggaggg ctgatagagg ctgacagcct ccttgagctg gggcagcatg tgctcgtcga
     2221 cgtagcggtg gatatcactg cggtagaggg ggttgaacca ttcgagcagg gagtaataaa
     2281 agcccatgcg cagtccgcgc gcgcgtacgg ccgtggtgag atcgccgagc agatcgcggt
     2341 ggggtccgac atcgacgctg ttccagttcc agctctgggt gttcggccac aggcaaaagc
     2401 cgtcgtggtg cttggaggtg aggaccacat agcgggcgcc ggcgcgttca aagagctcgg
     2461 cccacttgtc cgggcggtag agttcggcct tgaagagggg ggcgaaatcc tggtactgaa
     2521 agtcatcccc gtaggtgcgg tggtgccatt tgtgcaggaa ggtggaggtg tcggcgatac
     2581 cgttccagta ccattcggca tagcccccct tgcaggtgaa ggccggaacc gaatagagcc
     2641 cccagtgtat aaagatgccg aatttggcgt cgcggtacca ctgcggtgtc ggccgggcgt
     2701 cgagggattt ccagtcggcg gtgtagcgct cctgggccgg ggcgctggcg gcgagcagga
     2761 ggataacgag gagaatcttt ttcattctca cctcaaaaat attgcgtaca gattatcact
     2821 catacaaaaa gcgatctctc acggaggcac acaaagagga gctatcttat tatttctttg
     2881 tggcttggtg gcttggtgaa agctccgttt tctctgcgtt ctctgtgcca ccgtgtgaga
     2941 attttccatg cgtgc
//
LOCUS       DAOPWU010000405         2937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_34943_length_2937_cov_4.332061, whole genome shotgun sequence.
ACCESSION   DAOPWU010000405 DAOPWU010000000
VERSION     DAOPWU010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34943_length_2937_cov_4.332061"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2937
                     /locus_tag="PKM23_14355"
     CDS             <1..>2937
                     /locus_tag="PKM23_14355"
                     /inference="COORDINATES: protein motif:HMM:NF020698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY92703.1"
                     /translation="DDYINQVTVSGQYLGTEIPTYPSPLIFYATTAAGDADSILYVSG
                     NYQVGGINQPLPEPFKVKVTDAFGVPVSGHPVLFQAITPGTHFGGAAQVIRNTDASGI
                     ASAVGSIGSNFGNENYSFEARAEFNSMRLNGSPVQFTASGRRTTATKMVYLHGSGLAG
                     TVGRFLADSLSLRTLNASDKPVANQPVNFEVYSGSALLNGESNSLAAVSDVHGIARVA
                     LKLGSTPGVIKIRATADDGRVALANSPVDFEVTAHIGQPDGIRSQLTVTTPVIADGKA
                     AATIIVTLHDDQGNPVPGKTVALYTSGLDVHVKQPALATDSGGQTQGSITSTRAGALK
                     VWSMVENSIIPQDSAEVVFVPGPPASPIPFGTGQIALRGTGLPSPVGLYLYDANDNPV
                     PGIWVTFRVKSGGGSISQVQPVATGPDGKAQVNWILGSRIGEQYVAAVVPELGGREIE
                     FYAIANPPNPNSMTIVRGNRQIGLVNTALADSFVVAMTDSNAAPAEGLPVTFFKSKGQ
                     GDFLSPNPVTTDKRGYAAVLFKPGVVAGEYTVTAYHPSGLTGEFQFIVEERATVYLSK
                     VKDAKPGGRPNEEMTLQALVSDAYGKPLSGESLQWEILEGGGTLTTAAQVQSDALGQV
                     SVTWRLGLKGRQSVKLSPVTKAGGALSFSSQAVNAAPELTVPSDPSVLAGTPITFSIS
                     AFDADGDAITYGARSLPSGAAFDSTGKRSFTWTPTRAQAAGSPYMVTFIASDAFQAAD
                     TARIRITVTTLNTAPSIDSFEPGDTTLTRAFGQFIPFQIFASDAENDPLTYHWSVNGI
                     FGGDQTSFMWYPDKEIFPEECVVTVRVSDNKATRELNWHVHLITSAVELNSLTASAQK
                     GAVLLSWRTASESNHLGFNVLRSSLAEGPYETINAALISPAAEGRYSYLDRGARAGMK
                     YYYKIQDVDRSGLLTLHGPVAAEVALPERVDLAQNYPNPFNPVTTINFELPAAAEVEL
                     AIYDLQG"
BASE COUNT          541 a          989 c          873 g          534 t
ORIGIN      
        1 gatgattaca tcaaccaggt gacggtatcg ggacagtatc tcggcaccga gatcccgact
       61 tatccctcac ccctgatctt ttacgccacc acggccgcgg gagatgctga ttccatcctt
      121 tatgtcagtg gcaactacca ggtgggcgga atcaaccagc ctctgcctga gcccttcaag
      181 gtgaaggtga ccgatgcgtt cggcgtgccg gtctcaggcc atccggtgct ctttcaggcc
      241 atcaccccgg gcactcactt cggcggggct gcgcaggtca tccggaacac ggatgcctcc
      301 ggcatcgcca gtgctgtggg cagcatcggt tcaaatttcg gcaatgagaa ttacagcttc
      361 gaggcgcgcg ccgaattcaa ttcgatgcgg ctgaacggtt cccctgttca gttcaccgcc
      421 tcgggccggc gcacgactgc gaccaagatg gtctatctgc atggcagcgg cctggccggc
      481 acagtgggcc gttttcttgc ggactctctg agcctgcgca ccctcaacgc ctccgataaa
      541 ccggtggcca accagccggt taactttgag gtgtacagcg gcagcgcgct tctcaacggc
      601 gagtccaaca gcctggcggc cgtcagcgat gttcatggca tcgcccgcgt ggcgctcaag
      661 ctgggcagca cccccggcgt catcaaaatc cgcgccacgg ccgatgacgg acgggtcgcc
      721 ctcgccaatt caccggtcga tttcgaggtc accgcccata tcgggcagcc cgacggcatc
      781 cgttcccagt tgacagtgac cacgccggtg atcgccgacg gcaaggcggc ggccaccatc
      841 atcgtcaccc tgcacgacga ccagggcaat ccggtcccgg gcaagacggt cgccctttat
      901 acctcgggcc tggatgtaca tgtcaagcag ccggccctgg ccaccgacag cggcggtcag
      961 acccagggga gcatcacctc gacccgtgcg ggcgctctca aggtctggag catggtggag
     1021 aacagcatta ttccgcagga ctccgccgag gtcgtgtttg tgcccggtcc gcccgccagc
     1081 ccgatcccct tcggtactgg tcagatcgcc ctgcgcggca cgggactgcc ctcgccggtc
     1141 ggcctttatc tctatgacgc caacgacaat cccgttcccg gcatctgggt gaccttccgc
     1201 gtcaagtcgg gcggcggatc catatcacag gtacagccgg tggctacggg ccccgatggc
     1261 aaagcccagg ttaactggat tctcggcagc aggatcggcg agcagtatgt cgccgctgtg
     1321 gtcccggaac tgggcggcag ggagattgaa ttttacgcca tcgccaaccc ccccaatccc
     1381 aacagcatga ccatcgtacg cggcaaccgt cagatcggcc tggtcaacac cgcgcttgct
     1441 gactcgttcg ttgtggccat gaccgattcc aacgccgccc cggccgaagg actgccggtg
     1501 accttcttca agagcaaggg ccagggggat ttcctcagcc ccaacccggt caccaccgac
     1561 aagcgcggct atgccgcggt gctcttcaag ccgggtgttg tcgccggcga atacacggtg
     1621 accgcctatc atccctccgg gctgaccggg gaattccagt tcattgtcga ggagcgggcg
     1681 accgtctacc tgagcaaggt caaggatgcc aaacccggcg ggaggcccaa tgaggagatg
     1741 actcttcagg ccctggtcag cgatgcctac ggcaagcccc tctccggcga atcgcttcag
     1801 tgggagatcc tcgagggcgg cggcaccctg accacggcgg cgcaggtgca gagcgacgcc
     1861 ctgggacagg tgagcgtgac ctggaggctc ggcctcaagg gccgccagag cgtcaagctc
     1921 tcgccggtca ccaaggcggg cggagccctt tccttcagca gccaggcggt caatgctgcg
     1981 ccggaactga ccgtgccctc cgatccctcg gtcctggcgg ggactccgat caccttcagc
     2041 atctccgcct tcgatgccga cggcgatgcc atcacttatg gcgcgcgcag tctgccctcc
     2101 ggagccgcct tcgactcgac aggcaagcgc agcttcacct ggacgccgac gcgcgcccag
     2161 gccgcgggca gcccctacat ggtgaccttc atcgccagtg atgccttcca ggcggcggac
     2221 accgcccgca tccgcatcac cgtcacaacc ctgaacaccg ctccgagcat cgattccttt
     2281 gaaccggggg ataccaccct gacccgtgcc ttcggccagt tcatcccctt ccagattttt
     2341 gcgagtgatg ccgagaacga tcccctgacc tatcactggt cggtcaacgg catcttcggc
     2401 ggcgatcaga ccagtttcat gtggtatccg gacaaggaga ttttcccgga agagtgcgtt
     2461 gtgaccgtcc gcgtatcgga caacaaggcg acgcgcgaac tgaactggca tgtccatctc
     2521 atcaccagtg cggtggagct caactccctc actgcctccg cccagaaggg ggccgtactg
     2581 ctgagctggc ggaccgcctc cgagagtaat catctcggct tcaacgtgct gcgcagcagc
     2641 ctcgccgagg gcccctatga gaccatcaac gcggccctga tttcgcctgc tgcggaggga
     2701 cgctacagct atctcgaccg cggcgcccgg gcggggatga agtattatta caagattcag
     2761 gatgtggatc gcagcgggtt gttgaccctg cacggcccgg tcgcggcgga agtggccctt
     2821 ccggaacggg tggatctggc gcagaactat cccaatccct ttaatccggt gacgaccatc
     2881 aactttgagc tgccggctgc ggctgaggtg gaactcgcca tctacgatct gcaggga
//
LOCUS       DAOPWU010000406         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_35024_length_2932_cov_4.401807, whole genome shotgun sequence.
ACCESSION   DAOPWU010000406 DAOPWU010000000
VERSION     DAOPWU010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35024_length_2932_cov_4.401807"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKM23_14360"
     CDS             <1..226
                     /locus_tag="PKM23_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92704.1"
                     /translation="AGSRIPAVHPEASFAPRFLFIFPHHPLPSLVTPTFLSTPSPQGE
                     TFKQHCPRGFVHPAPSLQKKIFTKQLLKKG"
     gene            439..1719
                     /locus_tag="PKM23_14365"
     CDS             439..1719
                     /locus_tag="PKM23_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNY92705.1"
                     /translation="MIVHARDAQRGVEKDVKDRHSTRIGRTLSLLALLLMLASPSFAG
                     GLLVSWDPNTESDLSGYRIYYGTEPSVYSEMVDVGNVTSYTIENLVEGETYFMVVTAY
                     DLNGNESLPSAEVAATVTAAEIYITLLDEGIQIRWSALAGATLYRLYASDTPWFTLQS
                     PVAELTATSYTDVVNYRTVPFSRYYVVQAYAGASPMHSFDRVGVYSRGLRVGQNLVSM
                     PLLPADNSVGGVLGTQLTGAANAGQADKVLYWNGTDYEIAWLVEGTGTTFDGTWMTQA
                     GDQASPMKIDPDHSFWVLLRSSSPDTVLTFTGKVSKEANRVINLVQGANFIGTCYPVP
                     VTLAQSELAADGVVAGGSSSGVSDKIMRWLGNRYEVAWLVGGTGTGWDGTWLTESGFA
                     ASTIQFNPGVGYVLWIKGNNASKVWTYPNPRPNL"
     gene            1745..2821
                     /locus_tag="PKM23_14370"
     CDS             1745..2821
                     /locus_tag="PKM23_14370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92706.1"
                     /translation="MKRQIFAILLSVLMLGALAFSAAAGTFEAMSTTGILDADGTTFL
                     QGNVMLFSGDLVQLVWAGPNGVAEDPDSLGNATGDDAILDSTFIGYGFPFNPDEGKFS
                     VIWSDDLITLGNVVYLRAWNSPSVVPGEPVHFGSSELFTLPSSDMNSHDFRTFQVTQA
                     MHTPVELASFTASSFPGMIQVNWVTQSETNNLGFHLYRALSPHGEKELITEKMIEGAM
                     NSQSRHEYTFTDYRIDDQVVYYYWLADIALDGQITFNGPRTAVAVAKPTEYLLAQNYP
                     NPFNPSTSISYTLKDNGQVKLAIFNIRGQLIRSLVDQSQMAGQYSMEWDGRDANGSVV
                     PTGTYFYSLEINGFKAVRRMTMAK"
BASE COUNT          597 a          926 c          811 g          598 t
ORIGIN      
        1 cgccggttcc cgaataccgg ccgtccaccc ggaagcttcc tttgcgcccc gtttcctgtt
       61 catcttcccc catcatccgc ttccatccct ggttactccg acttttctct ctaccccctc
      121 accgcagggt gagacattca agcaacactg cccccgcggt tttgtccatc cagcgccctc
      181 cctgcagaaa aaaatattta ctaaacaatt attaaaaaag ggatgagcgc gcagcttaaa
      241 tctattatat aaagatttta cggctccatc aagccgctgc agaatttatt catcagcggc
      301 cctccgggat tggcacgaca tatgcctaag gggttgtgaa tggcggcatt tcatgtcaca
      361 gccggagggt cggggctgcc ggcaggggcc gcctgaaaaa accgaagtag gccttgaaag
      421 aaggagagga gcagattcat gatcgtacat gcgagagatg cccaaagagg cgttgagaag
      481 gatgtgaaag accggcattc tacccgcatt gggcgcacac tcagcctgct ggcgctgttg
      541 ttgatgcttg ccagcccaag ttttgccggc ggactgctgg tcagttggga tccgaatacg
      601 gaatccgatc tgtccggata ccgcatctac tacggcaccg aaccctccgt ctattcggag
      661 atggtcgatg tcggcaatgt cacctcctac accatcgaga atctggtgga gggcgagacc
      721 tacttcatgg tcgtcacggc ctatgatttg aacggcaacg aaagccttcc ctccgcggag
      781 gtcgccgcca cggtgaccgc agcggagatc tacatcacgc tcctcgatga gggcatccag
      841 atccgctgga gcgccctggc gggtgcgacc ctttaccggc tctacgcctc cgacaccccc
      901 tggttcacgc tgcagagtcc ggtcgccgag ctgaccgcaa cctcttatac cgacgtggtc
      961 aactaccgga ccgtgccctt cagccgctac tacgttgtgc aggcctatgc cggggccagt
     1021 ccgatgcatt ccttcgaccg ggtcggggtc tacagccggg gattgcgggt gggccagaac
     1081 ctggtctcga tgcccctctt gcccgcggac aacagcgttg gcggcgtgct cggcacccag
     1141 ctcacaggag cggccaatgc cggccaggcc gacaaggtcc tctactggaa cggcacagac
     1201 tatgagatcg cctggctggt ggagggaacc ggcaccacat ttgacggcac ctggatgacc
     1261 caggccggcg atcaggcctc cccgatgaag atcgatcccg accactcttt ctgggtgctg
     1321 ctgcgcagct ccagtcccga taccgtgctg accttcaccg gcaaggtgag caaggaggcc
     1381 aaccgggtga tcaatctggt gcagggcgcc aactttatcg gcacctgcta tccggttccg
     1441 gtcaccctgg cccagagcga gctcgccgcc gatggtgtcg tcgccggcgg ttcctccagc
     1501 ggtgtgtcgg acaagatcat gcgctggctt ggcaaccgct acgaggtggc ctggctggtg
     1561 ggtggaaccg gaaccggctg ggacggcacc tggctgaccg aaagcggatt cgccgcctcg
     1621 accattcaat tcaatcccgg tgtcggatat gttctgtgga tcaagggcaa caatgcgtcc
     1681 aaggtctgga cctaccccaa tccgcggcct aatctttaat acatctgtct gaaggagaaa
     1741 tgagatgaaa cgccaaattt ttgccatcct gctttccgtc ctcatgctgg gggcgctggc
     1801 tttctccgct gcggcgggca ctttcgaagc catgtccacc accggcatcc tcgatgccga
     1861 cggcaccacc tttctgcagg gcaacgtcat gctcttcagc ggcgacctcg tccagctggt
     1921 ctgggccggg cccaacggtg tcgctgaaga tccggactca ctcggcaatg ccaccgggga
     1981 tgatgccata ctcgacagca ccttcatcgg ctacggtttt cccttcaacc ccgatgaggg
     2041 caagttcagt gtcatctgga gtgatgatct gatcaccctc ggcaatgtcg tctatctgcg
     2101 cgcctggaac agtccgtctg tggtccccgg cgagcccgtg cactttggca gctcggagct
     2161 cttcaccctg ccttcgagcg acatgaacag ccacgacttc cgcacctttc aggtgactca
     2221 ggccatgcac accccggtcg agctcgcctc gttcaccgcc tcctcgtttc ccggcatgat
     2281 ccaggtgaac tgggtgaccc agtcggagac caacaacctc ggtttccatc tctaccgcgc
     2341 gctgtcgccc catggggaaa aggagctcat caccgaaaag atgatcgagg gcgcgatgaa
     2401 ctcgcagagc cgccacgagt acaccttcac cgattatcgc atcgacgacc aggtggtcta
     2461 ttactattgg ctggcggata tcgcgctgga cggccagatc accttcaacg gaccgcgaac
     2521 ggctgtggcc gtcgccaagc cgaccgagta tctgctggcg cagaactatc ccaacccctt
     2581 caatccttca accagcatca gctataccct gaaggataac ggccaagtca agctggcgat
     2641 ctttaacatc cgcggccagc ttattcgttc gctggtggac cagtcccaga tggccggaca
     2701 gtacagcatg gagtgggacg gccgcgacgc caacggcagc gtggtgccga ccggaaccta
     2761 cttttattct ctcgagatca acggcttcaa ggcggtgcgc cgtatgacca tggccaagta
     2821 gcagcggcat gaatccgcgc aggcctgcaa atcagactca aggagatatg atgaagctgc
     2881 acgctctgaa cataaactcg ctgcggggag tgatcctgct cctcctgatc ct
//
LOCUS       DAOPWU010000407         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_35230_length_2918_cov_4.241006, whole genome shotgun sequence.
ACCESSION   DAOPWU010000407 DAOPWU010000000
VERSION     DAOPWU010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35230_length_2918_cov_4.241006"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PKM23_14375"
     CDS             complement(<1..655)
                     /locus_tag="PKM23_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92707.1"
                     /translation="MKLFLKMMVAVLAVLLLSGLAFSQVKFDFENAAEGTQNWGIWWG
                     NAGTALAQAADPSGRSTGVLKLSCTAATGDGKAAIGVDPLPLGWTAADVQGAKFFTID
                     VYVPEGFPDSAVVKLWAQIAEGSWQWTDYKWSIAGEGNHFKAGQWVTLHYPVSAMMEA
                     YANFQPWLKIKGGVEVYFPSDKSSSPTWAGDVLFDNFALAGVEPVVVTDFTANNGNFG
                     "
     gene            complement(816..1823)
                     /locus_tag="PKM23_14380"
     CDS             complement(816..1823)
                     /locus_tag="PKM23_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNY92708.1"
                     /translation="MTTIKNIAKAAHCSAATVSKALNGRPDVAEATRQRILAIAREHD
                     FHPNALGKALKKKITESVGVIFCREQLPLSLNPFYSRVLEGIEAELAVSNHSLVLHLL
                     PEGHTTAIPKMIRERQVDGLILIGVLQQAFIDQLASLEIATVLVDPKITSNTFSQVLI
                     DNEHGAFQAVQFLLDHGHTRIGFISGDLDRLSFRQRLDGYCKALEFARLPLCEELVRC
                     GGLEKGYDHLSALLDLPEPPSAIFAANDINAIYGYKAVTDHGLSVPDDISIIGFDDIE
                     LGKMISPPLSTIRVYKEQMGSIAVRTLFKVLNHEIVQPVTTLVPTRLIERESVAARVS
                     A"
     gene            complement(2172..>2918)
                     /locus_tag="PKM23_14385"
     CDS             complement(2172..>2918)
                     /locus_tag="PKM23_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNY92709.1"
                     /translation="GPSVQAALRRGEAPKVVYFPSCITRSMGPARGDSAGEDLPHLTE
                     RLLHKAGFEIIYPDRMDELCCGMAFASKGFREAGDAKAAELNAALWRASAEGKYPVLA
                     DMSPCLYRMKETLDERLRLYEPIAFTLEHLAPRLDFHPEPETVMIHAVCSAKKMDLEG
                     PFFTLARMCAARVVATEGNCCGFAGDRGFTYPELNAHGLRHLHEQVPEGCAEGFSTSR
                     TCEIGLSTHSGIPFKSILYLVDRCTTPKPL"
BASE COUNT          599 a          785 c          929 g          605 t
ORIGIN      
        1 tgccgaaatt gccattattg gcagtgaaat cggtcaccac gacgggctcg acacccgcca
       61 gggcgaaatt atcgaagagg acatctccgg cccaggtcgg acttgaactt ttatcactcg
      121 ggaaataaac ttctacgccg cccttgatct tgagccaggg ctggaaattg gcataggctt
      181 ccatcatcgc cgaaacggga tagtggaggg tgacccactg gccggccttg aaatggttgc
      241 cctcgccggc gatggaccat ttatagtcgg tccactgcca ggagccttct gcaatctggg
      301 cccagagctt gacgacggcg gaatcgggaa aaccctcggg gacatagaca tcgatggtga
      361 agaatttggc accctgcaca tcagcggcgg tccagcccag gggcagggga tcgacgccga
      421 tggcggcctt gccgtcgcca gtggcagcgg tgcacgagag cttcaggacg ccggtgctgc
      481 gtccggaggg atcggcagcc tgcgccagag cggtgccggc attgccccac cagatgcccc
      541 agttttgcgt gccttctgcg gcgttctcaa aatcaaattt gacctgagaa aaagccagtc
      601 cgctgagcag caaaaccgcg agcactgcca ccatcatttt caaaaacagt ttcatgcttg
      661 cctcctgata cgttttggat ggatgaaacc gactgtagta aaatggactg cttcgttgca
      721 tcaacacact ctctgcaacc gaggctcacc tccttccttt tcgtgcaata gaatcccgga
      781 acttgtgtgg ccctgccggc ggagcggtgg tctgctcacg ccgatacgcg tgcggccacg
      841 gattcccgtt cgatcagccg ggtcgggacg agggtggtga ccggctgcac tatttcatgg
      901 ttcagcacct tgaagagggt gcgcactgcg atggaaccca tctgctcctt gtaaacgcgg
      961 atggtggaga ggggcggcga gatcatcttg cccagctcga tgtcatcgaa gccgatgatg
     1021 gagatatcgt caggaacgct gaggccgtgg tcggtgaccg ccttgtagcc ataaatggca
     1081 ttgatgtcgt tggcagcgaa gatggcactc gggggttcgg gcaggtcgag gagggcgctg
     1141 agatggtcat agcccttctc caggccgccg cagcgcacca gctcctcgca gagagggagt
     1201 cgggcgaatt cgagggcttt gcaataaccg tcaaggcgct ggcggaagct gagccggtcg
     1261 agatcgccgg agataaaacc gatgcgggta tggccatgat caagaaggaa ctggacggcc
     1321 tgaaaggcgc cgtgttcgtt gtcgatcagc acctgggaaa aggtgttgct ggtgatcttg
     1381 gggtcgacca gaacagtggc gatctcgagt gaagcgagct ggtcgataaa cgcctgctgc
     1441 agcaccccga tgagaatgag gccgtcgacc tgacgctcac ggatcatctt gggaatggcg
     1501 gtggtgtgtc cctcggggag aagatggagg acgaggctgt ggttgctgac ggcgagttcg
     1561 gcctcgatgc cctcaagcac gcgtgaataa aaaggattga gggagagggg cagttgttcg
     1621 cggcagaaga tgaccccgac gctttcggtg atttttttct tgagggcctt gccgagggcg
     1681 ttgggatgaa aatcgtgttc cctggcgatg gcgaggatgc gctggcgggt ggcctcggcg
     1741 acatcggggc ggccgttgag ggccttggag acggtggccg ccgagcagtg ggcggccttg
     1801 gcgatgtttt ttatggtagt catcaccctc tggcgaaagg gggaaggggc gggagacggt
     1861 ggattatttc gaaatcgttt tcgttatcct aaaaaaaagt agcacttttt ttgctttcag
     1921 tcaagcattt tttcgaaatt attaaatatt ttcgaagatt gggggcgggc agtgagacca
     1981 gaaagttttt tattgttgtt aattatgttt ttacgatctt cgcaaggaat aacgatttcg
     2041 aaatcgtttt cagcggaacg gggcgcttcc ctcccgctcc atcgaaaatg gcccatgtta
     2101 catcccggat gcagattaat gctcgccagc agcaggccgc tggcgcaccg ggatccgccc
     2161 cggcaacacg ctcagagcgg tttgggcgtg gtgcagcggt cgaccagata aagtatcgac
     2221 ttgaagggga tgccgctgtg ggtggagaga ccgatctcgc aggtccggct ggtcgaaaag
     2281 ccctctgcac acccctcggg cacctgttca tgcagatggc gcagaccgtg ggcattgagt
     2341 tcgggatagg tgaaaccacg atcgccggcg aagccgcagc agttgccctc tgtcgccacc
     2401 actctcgccg cgcacatgcg cgccaatgtg aaaaagggac cctccagatc catctttttg
     2461 gcactgcaga cggcgtggat catcaccgtc tccggttcgg gatggaaatc caggcgcggt
     2521 gccagatgct caagggtgaa ggcgataggt tcatagagcc tcagccgctc gtcgagagtc
     2581 tccttcatcc ggtagaggca ggggctcatg tcggcgagca ccggatattt tccctctgcg
     2641 ctcgcccgcc acagggcggc gttgagctcc gcggccttgg catctcccgc ctccctgaag
     2701 cccttgctgg cgaaggccat gccgcagcag agttcgtcca tccggtcggg atagatgatc
     2761 tcaaaacccg ccttgtgcag cagccgttcg gtcaaatggg ggaggtcctc tccggcgctg
     2821 tccccccgtg cgggacccat tgagcgggtg atacaggagg gaaagtagac caccttgggc
     2881 gcctcccccc tgcgcagcgc agcctggacc gaggggcc
//
LOCUS       DAOPWU010000408         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_35396_length_2908_cov_4.410095, whole genome shotgun sequence.
ACCESSION   DAOPWU010000408 DAOPWU010000000
VERSION     DAOPWU010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35396_length_2908_cov_4.410095"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..1258
                     /locus_tag="PKM23_14390"
     CDS             479..1258
                     /locus_tag="PKM23_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92710.1"
                     /translation="MKHYFLCLLPLSLLFSFSLLAQPVDHRRLIYSHPLTSAAPGYGE
                     AIYNTDGEFDAVKGWQAVHTTSQLQIHLAKPLPAEGTLAIKVTNFNPAEQWVDDLKLH
                     IVNLYSRLYTNNKEIFDTDGSWIQIRTGSGYSTMPGRAGFKVLAAARGIGTRDEVTCV
                     EDYLWKTSRIYEFRITWTRGRVYLFLDNNLMAELSFLGQIEPFKYILIGKDNLIWGYC
                     AQPGPWYFDLRIYEKGEAVVEDRVPPAAPAQVTVRPLAEVR"
     gene            complement(1271..1417)
                     /locus_tag="PKM23_14395"
     CDS             complement(1271..1417)
                     /locus_tag="PKM23_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92711.1"
                     /translation="MQVGETTITWDDRINQVANMYGELLIDYAHYLFEERFIVGFGGS
                     RNCC"
     gene            1835..>2908
                     /locus_tag="PKM23_14400"
     CDS             1835..>2908
                     /locus_tag="PKM23_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNY92712.1"
                     /translation="MKVQTTCPFCGCGCGMLLEVEKGVVLAAAPQRDHPVSRGTLCIK
                     GWNGHQLIHHPERLTQPLLRINDCLKPVSWNKALSYAAQRITELQQAHGRDCVGVVGS
                     LKTVNEASYLLGKLARTVLHTPHLDAPLRFDHAPSLRVLREQSGFANAAAALADIEKA
                     GAILVIGADAKAQNARAGSLLLQAAKRGVPVIQVDPREQEHSRFFRLHLRPRPGTDLA
                     LINALSHILLAEGWQAEGLAGLAKLRDDGLERFTPAYAESVGGIAQDDLRSAAGLIAQ
                     ASSLLILFGTGITQQANGTANVRALWNLALLTGSIGREGGGLLPLLTANNSQGAVDTG
                     LMSEWGPGQRDLQETAVRRAIEAA"
BASE COUNT          599 a          875 c          822 g          612 t
ORIGIN      
        1 cggcgaaccc tgtcgatgga tggattcaga acagccccgc gcacacaaac cggaaacggc
       61 cgaaatgggc gtagacaagt cccacacggc acgatgttga ttcccctggt cgtgctgaga
      121 agagttgcga acaaaatcag ttggctaggt actgcggata aaactgagga agctgcggtg
      181 ctaaggatgc cgttgcgagg gtgtgatcac ttgtggtgct cttgatcata tgaacaaata
      241 taaggtaata atattccaaa ttaaagtaat atttttaaat aatcgtcata aagaacgaca
      301 tttattttaa tatttacaat gcactttaca ttcctaaaac cctggcctgc tatttgcttt
      361 caaggttgta atcggcgccc cggccgggca gccacctgaa taagataaat aaatacaata
      421 ctcacgcccc cgtcgtcagc acacctgtgg cggtgaggcc acactatgtg aggggttcat
      481 gaaacactat ttcctctgtt tgctgccgtt atccctgctc ttttcatttt cgctgctggc
      541 ccagccagtg gaccaccggc gcctcatcta cagccatccc ctgacctcgg ccgctccggg
      601 ctacggggag gctatctaca acaccgacgg agagttcgac gcggtcaagg gctggcaggc
      661 tgtccatacc accagtcagc tgcaaatcca cctcgccaaa cccctgcccg cggaggggac
      721 gttggccatc aaggtgacca acttcaatcc ggccgagcag tgggtggatg acctcaagct
      781 gcacatcgtc aatctctatt cccggcttta caccaacaac aaggagatct ttgataccga
      841 cggctcctgg atccagatcc gcaccggcag cggctactcc accatgccgg gccgggctgg
      901 tttcaaggtg ctggctgcag cgcgcggcat cggcacccgg gatgaggtca cctgtgttga
      961 ggattacctt tggaagacaa gccgcatcta cgagttccgc atcacctgga cgagggggcg
     1021 tgtctacctc tttctggaca acaacctgat ggcggagctc tcttttttgg gacagatcga
     1081 gcccttcaag tacattctga tcggcaagga taatctgatc tggggctatt gtgcgcagcc
     1141 gggaccctgg tatttcgatt tgcgtatcta cgaaaagggg gaagcagtgg tggaggatcg
     1201 ggtgccaccc gccgcgccgg cccaagtcac tgtccggccg ctggcggagg tccgatagga
     1261 gggaggaggc tcagcagcag ttgcggctgc cgccgaatcc gacgatgaag cgttcttcga
     1321 agagataatg ggcgtagtcg atgagaagct cgccgtacat attggcgacc tgattgatgc
     1381 ggtcatccca ggtgatggtg gtttcgccga cctgcatctg atttgcggtg tggggttcga
     1441 tatgggcagc ctcgaccagg ctgagcttgg ggccgcctca gccaaagccg gcgatgacga
     1501 cctgatagag acgcggtccc ttttccgcag ccttgaggag ggctttttct gcacgttcgg
     1561 ttaatttgat cattcccgcc tggttctcca tattattctt cacgggccgt gtaccggcca
     1621 cctgcattcg ttatgcattc agattatcaa attttcgtca aataagcaag ccttttcgtc
     1681 gctccctgat gctgtgtccg acgaagtgcg gcgcggatgc atccgtaaaa aaattaaaaa
     1741 gtacttgata tagtctcgct gattatttat actaaactat gcgcgcccat ctggtttgga
     1801 tcgacctggc gaatctctaa agtgggagta gcaggtgaaa gttcaaacga cgtgtccctt
     1861 ttgcggatgt ggctgtggaa tgctcctgga ggtggaaaaa ggcgtggtac tggccgccgc
     1921 gccgcagcga gaccaccctg tcagccgggg aaccctctgt atcaaggggt ggaacggcca
     1981 ccagctcatc catcaccccg aacgcctcac ccaacccctt cttcgcatca acgactgcct
     2041 caaacccgtc agctggaaca aagccctctc ttatgccgcc caaaggatca ccgaactgca
     2101 gcaggcccat ggccgggact gtgttggagt agtcggttcc ctcaagacgg tcaatgaggc
     2161 ctcctatctt ctcggcaagc tggcgcggac ggtgctgcac accccccacc tcgatgcgcc
     2221 gcttcgtttt gaccatgcgc cctccctgcg cgtcctgcgc gagcagagcg gctttgccaa
     2281 tgcggccgcc gcccttgccg acatcgagaa ggcgggggcg atcctggtca tcggcgccga
     2341 tgccaaggcg cagaacgccc gcgcgggctc cctgctcctc caggccgcca aacggggtgt
     2401 cccggtcatt caggtcgacc cccgcgagca ggagcacagc cgttttttcc gcctccatct
     2461 tcggccccgg cccggcaccg acctcgccct gatcaacgcc ctcagccata tcctgctcgc
     2521 agaaggctgg caggcagaag gccttgccgg gctggccaag ctgcgcgatg acggccttga
     2581 gcgcttcacc cccgcctatg ccgagagcgt cggtggcatc gcgcaggatg atctccgctc
     2641 cgccgccggt ctcatcgccc aggcttcttc cctgctcatc ctcttcggca ccggaatcac
     2701 ccagcaggcc aacggtacgg ccaacgtccg ggccctgtgg aatctcgccc tgctcaccgg
     2761 cagtatcggc cgcgagggcg gggggctgct gccgcttttg actgccaaca acagccaggg
     2821 agcggtcgat accggcctga tgagcgaatg gggaccgggc cagcgcgatc tgcaggagac
     2881 ggcggtccgc cgggccattg aggcggcc
//
LOCUS       DAOPWU010000409         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_35727_length_2889_cov_4.145730, whole genome shotgun sequence.
ACCESSION   DAOPWU010000409 DAOPWU010000000
VERSION     DAOPWU010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35727_length_2889_cov_4.145730"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PKM23_14405"
     CDS             complement(<1..613)
                     /locus_tag="PKM23_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNY92713.1"
                     /translation="MKKDIIINISIGESRVAILEDGRLAELYLEKPENERMVGDIYYG
                     KVAKVIKGMQAAFIDIGLNQDAFLHFSDVSENLASFNQIFPKEAPAPTRGGGNNSNNS
                     NNSNSGQRQHHHGRDYNEPQLRTGQPILVQVTKEPISNKGARVTTEISLPGRFLVLIP
                     YDDMIGVSRKITNRRERARLKQLGRAIKPQGFGLVIRTVSEGKD"
     gene            complement(628..>2889)
                     /locus_tag="PKM23_14410"
     CDS             complement(628..>2889)
                     /locus_tag="PKM23_14410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNY92714.1"
                     /translation="ADRQEGPLIVGGGPSVFNPEPVAEFFDLLVIGDGEEVLLELTAA
                     VARARREGQSRGALLRACAQIPGVYIPQLYHPHLNNAGAFAGLETLQTEAPARIRSRI
                     VSGLDRSHYSHRPLVPVIATTHDRVSLEIARGCSRGCRFCNAGMIYRPVRQRSVDDLV
                     EQAVESIRNTGYDEVSLVSLSTSDYEELGPLMERLQAGLGERMVNISFPSLRPEKFTP
                     EVARFARGVRKSGLTLAPEAGSERLREVINKTTTSDDLLRAVDLAFREGWKVVKLYFM
                     IGQPTEEDEDLQGMADLIRQVVALARRRSGIRINVSVSPFVPKPGTPFQWVAQDSPEE
                     TRRKLAFLRDRVRDKLVKLSWRDAEVAQIEGVLARGDRRLGRVILRAWQSGATLEGWS
                     ENFHHETWLRALAEEGLQPEQFTGGYPLDAPLPWEHIDKGVTARFLQDEYRRALGQEV
                     TPDCRDGQCNRCGLMAQPVCQEILRGSKNIRSGSGRQESAGPATAGPEEAGTPPDPGR
                     EAGAVAHPPAQNTEEGRWVRLHYQRGEEVRWISHLDFIHVFERALRRADWPLVYSEGF
                     NPHPRIAYGPPLPTGQISRAEYLDLMVRGNETGAHVAALAAQLPAGILIREARLLPAK
                     PRALADEIVRSVCRVTWPTMAEPEDLKARIEALMGSSEAWVTRRKEKEPPRRIDVRPF
                     IGGLTRQEGALQIVSHIVHGKTVRMEEIIRLLFPDEPGRAQLATIEREHLWMEKEGRL
                     ITPLEAVEGGIRV"
BASE COUNT          477 a          931 c          945 g          536 t
ORIGIN      
        1 catccttgcc ctcggagacg gtgcggatga ccagtccgaa gccctgcggt ttgatggcgc
       61 ggccgagctg tttgaggcgg gcgcgctcgc ggcggttggt gatcttgcgc gaaaccccga
      121 tcatgtcgtc gtaggggata agcaccagaa aacgcccggg cagggaaatt tcggtggtga
      181 cgcgcgcccc cttgttcgag atcggctcct tggttacctg caccaggatg ggctggccgg
      241 tgcgcagctg cggttcgtta taatcgcggc cgtgatggtg ctggcgctgg ccgctattgc
      301 tgttattgct gttattactg ttattaccgc cgccccgcgt gggggcagga gcctccttcg
      361 ggaaaatctg gttgaaggag gcgagattct cgctgacgtc ggaaaaatgg agaaaggcgt
      421 cctggttgag gccgatgtcg ataaaggcgg cctgcatccc cttgatcacc ttggcgacct
      481 tgccgtaata gatgtcgccg accatgcgtt cattttcggg tttttcgaga tagagctccg
      541 ccagccgccc gtcttcaagg atcgcgacgc gcgactcgcc gatggagatg ttgatgataa
      601 tgtctttttt catgatgcct catttcttta cactctgatg ccgccctcca ccgcctcgag
      661 gggagtgatg agtctcccct ccttttccat ccagaggtgc tcccgttcga tggtggccag
      721 ctgcgcgcgg ccgggctcgt cggggaagag caggcgaata atttcttcca tacgcacggt
      781 cttgccgtga acgatatggc ttacgatctg cagtgcgccc tcctgccggg tcaggccgcc
      841 gatgaagggg cggacgtcga tgcggcgggg gggctctttc tcttttctgc gcgtcaccca
      901 ggcctcgctg ctccccataa gggcttcgat gcgcgctttt aaatcttcag gttccgccat
      961 ggtgggccag gtcacccgac acacggagcg aacgatctca tcagccaggg cgcgcggttt
     1021 ggccggcagc agccgggcct cccggatgag gatcccggcc ggcagctggg ccgccagggc
     1081 ggcgacatgc gcgccggtct cattccccct gaccatcaga tcgagatact cggcccggct
     1141 gatctgcccc gtgggcagcg gcggtccgta ggcgatgcgg gggtgcggat tgaacccctc
     1201 actgtagacc aggggccagt cagcccggcg cagcgcccgt tcgaaaacat gaatgaaatc
     1261 gaggtgcgaa atccagcgca cctcctcccc tcgctgataa tgcagccgca cccagcgccc
     1321 ctcctcagtg ttctgcgcag gggggtgggc gaccgcaccg gcctcccgcc ctggatcagg
     1381 cggcgttccg gcctcttccg gcccggccgt cgccggccca gccgactcct ggcggccgct
     1441 gccggagcgg atattcttgc tcccccgcag aatctcctgg caaacgggct gggccatgag
     1501 gccacagcgg ttgcactggc cgtcgcggca gtcgggcgtc acctcctgcc cgagtgcgcg
     1561 gcggtactca tcctgcaaaa atctggcggt cactcccttg tcgatatgct cccagggcag
     1621 gggcgcgtcg aggggatagc ccccggtgaa ctgttccggc tgcagcccct cttcagcgag
     1681 agcgcgcagc caggtttcgt gatgaaaatt ctccgaccat ccctccaggg tggcgccgga
     1741 ctgccaggcg cgcaggatga cccgcccgag ccggcggtcg ccgcgagcga gcaccccctc
     1801 gatctgggcc acctcggcgt cccgccagct cagcttgacc agtttgtcgc gaacgcggtc
     1861 gcgcagaaag gcgagcttgc gccgggtctc ctcggggctg tcctgggcga cccactgaaa
     1921 gggcgtgccc ggcttgggga cgaagggcga gaccgaaaca ttgatgcgga tgccgcttcg
     1981 ccgccgtgcc agggcgacga cctgcctgat gagatcggcc atcccctgca ggtcttcatc
     2041 ctcttcggtc ggctggccga tcatgaaata gagcttgacc accttccagc cttcgcggaa
     2101 ggccagatcg acggcccgga gcagatcgtc ggaggtggtg gttttgttga taacctcgcg
     2161 cagccgttcg ctccccgcct cgggggcgag ggtcaaaccc gacttgcgca cccccctggc
     2221 aaaacgggcc acctcggggg tgaatttctc cgggcgcagc gaggggaagg agatattgac
     2281 catcctctcc ccgagcccgg cctgaagcct ctccatgagc ggccccagct cctcataatc
     2341 cgaggtcgag agcgaaacca gtgaaacctc atcgtagccg gtattgcgga tgctctcgac
     2401 cgcctgctcg accagatcgt cgacactgcg ctgccggacc ggacggtaga tcatgccggc
     2461 gttgcaaaag cgacagccgc gcgaacagcc gcgggcgatc tccagggaga cgcgatcatg
     2521 ggttgtggcg atgaccggca ccagcggccg gtgtgaatag tggctgcgat cgagtccgga
     2581 gacgatgcgg ctgcggatgc gcgccggcgc ctccgtctgc agggtttcca gtccggcaaa
     2641 agcgccggca ttgttcaggt gcggatggta gagctgcgga atgtataccc cgggaatctg
     2701 cgcgcaggcg cgcaggagtg ccccccgtga ttgaccctcg cgccgggccc tggccacggc
     2761 tgccgtgagc tcgagcagga cctcctcgcc atcgccgatg accaggaggt cgaaaaactc
     2821 ggcgacgggt tcgggattga aaaccgaggg gccgccgccc acgatgaggg gcccctcctg
     2881 ccggtcggc
//
LOCUS       DAOPWU010000410         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_35729_length_2889_cov_3.518349, whole genome shotgun sequence.
ACCESSION   DAOPWU010000410 DAOPWU010000000
VERSION     DAOPWU010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35729_length_2889_cov_3.518349"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKM23_14415"
     CDS             complement(<1..630)
                     /locus_tag="PKM23_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNY92715.1"
                     /translation="MESTLLIDHATIISPGNPDRVLHDHALLIESGAIKAVAPSEALR
                     AGSSITQETAVTPGSLLGARIIDAAGKILMPGLINAHTHCYSTFARGLTKTAPAESFE
                     AVLENLWWRLDKALTLEDCYWSALSVLLEAVRSGTTTLIDHHASPRAIRGSLEAVARA
                     FRATGLRGCLCYEVSDRDGAAAARAGLEENTAFIRTCRERCDPRIAAMFG"
     gene            complement(640..2796)
                     /locus_tag="PKM23_14420"
     CDS             complement(640..2796)
                     /locus_tag="PKM23_14420"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNY92716.1"
                     /translation="MPRPAAPAPNGKQVAVVGAGPAGLAAAAYLARLGYAVTVFDQES
                     QPGGTCNWIPEHRLPRDVVQSDIEWLLSLGLIELKLGTKVSHVPALLSQGYQGVVVAT
                     GLMKPFVMGIPGEEQALAGLTYLKNPGAHPLSGKVAVIGGGATAVDCAVTAKLQGASA
                     VELICLERWDEMPLTTAEREELQQHGIELLGRTRLTAIRGTGQNLGLELEKVTLAPGA
                     KFSVPGLRKVEKTASQRWDFNHVIVAIGARSELAKVESAQIVWAGDAHTGPATVVQAS
                     ASGKNAAQELHASLSGEPRPVIAKHTKSRTPLPGYSMLPVPLDCDFFGRPLRSPFLLS
                     AAPPSDGYDQMRKAYEAGWAGGVMKTAFDGVPIHIPARYMHAFDEKTYGNCDNVSGHP
                     LDRVCREVEALVREFPDRLTLASTGGPVTGGDDADRAAWQSNTRKLESAGAMGIEYSL
                     SCPQGGDGTRGDMVSQDAELTAKIIGWVLEAGDAEIPKLFKLTAAVTAIGPIAAAIEA
                     VFRRHPGKKAGITLANSFPAMAFRKAPGRRWEEGVVIGLSGEGVLPISNYTLARVAGM
                     GLAVSGNGGPMDYNAAANFLALGARSVQFCTIVMKYGYGIVGELHSGLSFLLQERGLR
                     SVAELIGSALPDPITPFEALSSRKLIPQVDAGLCEHCGNCTRCPYQAIALDRKRVPTF
                     DAACCVGCSLCAQKCFSGAITMRERSEAEAAALQEK"
BASE COUNT          436 a          942 c         1044 g          467 t
ORIGIN      
        1 cccgaacatc gccgcgatgc ggggatcgca gcgctcccgg caggtgcgga taaaggcggt
       61 gttctcctcc agccccgcgc gggccgcagc ggcgccgtcg cggtcggaaa cttcgtagca
      121 gaggcagccg cgcaggccgg ttgcacggaa agcccgggcg acggcctcga gcgagccgcg
      181 gatcgcgcgg ggactggcgt gatgatcgat cagggtggtg gtgccgctgc ggactgcctc
      241 cagcaggaca gagagggcgc tccagtagca gtcctcgagg gtgagggcct tgtcgagccg
      301 ccaccagaga ttctccagca ccgcctcgaa cgactccgcc ggcgcggttt tggtcaaccc
      361 gcgcgcaaag gtgctgtagc agtgggtgtg ggcgttgatc aggccgggca tgagaatttt
      421 tccggctgcg tcgatgatcc gggcgccgag caggctgccg ggagtgactg ccgtctcctg
      481 agtgatggaa cttccggcgc ggagtgcttc ggagggtgcg acggccttta tcgctcccga
      541 ctcgatgagg agggcgtggt cgtggagcac cctgtccgga ttgccaggtg agataattgt
      601 ggcgtgatcg atgagcaggg tggattccat tttacctcct tatttttcct ggagtgcggc
      661 cgcttccgcc tcgctgcgtt cgcgcatggt gatcgccccg ctgaagcact tttgcgcgca
      721 cagcgaacag ccgacgcagc acgcagcatc gaatgtcgga acgcgcttgc gatcgagagc
      781 gatggcctga taggggcagc gcgtgcagtt gccgcagtgc tcacagaggc cggcatcgac
      841 ctgcggaatc aacttgcggc tgctcagggc ctcgaaagga gtgatgggat cgggcagggc
      901 ggagccgatc agctccgcca ccgagcgcaa gccgcgctcc tgcaggagaa agctgagccc
      961 ggaatggagt tcgccgacga tcccatagcc gtatttcatc acgatggtgc agaactgcac
     1021 gctgcgggcg ccgagggcga gaaagttggc cgccgcgttg tagtccatcg ggccgccgtt
     1081 acccgagacc gccaggccca tgccggcgac gcgggcgagg gtgtaattgc tgatcggcag
     1141 gacgccttca ccggagaggc cgatgacgac gccctcctcc cagcgccggc ccggggcctt
     1201 gcggaaggcc atggccggaa aactgttggc cagggtgatg cctgccttct tgccgggatg
     1261 gcggcggaaa accgcctcga tggcggcagc gatcgggccg atggcggtga cggcggcggt
     1321 gagcttgaag agcttgggga tctcggcatc gcccgcctcc agaacccagc cgatgatttt
     1381 ggcggtcagc tctgcgtcct gtgagaccat atcgccgcgg gtgccgtcgc cgccctgggg
     1441 gcaggagagg ctgtattcga tgcccatcgc cccggccgat tcgagcttgc gggtgttgga
     1501 ctgccatgcc gcccggtcgg cgtcgtcgcc gccggtcacc ggtccgccgg ttgaggccag
     1561 ggtgaggcgg tctggaaact cccggaccag ggcttcgacc tcgcggcaga cccggtcgag
     1621 gggatggccg gagacgttgt cgcagttgcc ataggtcttc tcgtcgaacg cgtgcatgta
     1681 gcgcgcgggg atgtggatcg gcaccccgtc gaacgcggtc ttcatgacgc cgccggccca
     1741 gccggcctcg taggccttgc gcatctggtc gtagccgtcc gagggcggcg cggccgacag
     1801 caagaagggc gagcggagcg ggcgaccgaa gaaatcgcag tccaggggca cgggcagcat
     1861 cgaatagccg ggcaggggag tccggctctt ggtgtgcttg gcgatcaccg gccgcggctc
     1921 cccagagagg gaggcatgca gctcctgcgc ggcattcttg cccgaggccg aggcctggac
     1981 gacggtggcc gggccggtgt gcgcgtcgcc ggcccagacg atctgcgcgc tctcgacctt
     2041 ggccagctcg ctccgggcgc cgatggcgac gatgacgtgg ttgaagtccc agcgctggga
     2101 cgcggtcttc tcgaccttgc gcagccccgg cacgctgaac ttggccccgg gcgcgagggt
     2161 caccttctcg agctccagcc cgaggttctg gcccgtgccc cggatcgcgg tcaggcgggt
     2221 gcggccgagc agctcgatac cgtgctgctg cagctcctcc cgctcggccg tcgtcagggg
     2281 catctcgtcc cagcgctcca ggcagatcag ctcgaccgcg ctcgcgccct ggagcttcgc
     2341 ggtcaccgcg cagtccaccg cggtcgcgcc gccgccgatc accgcgacct tgcccgacaa
     2401 ggggtgggcc ccggggttct tgaggtaggt cagcccggcc agggcctgct cctcgcccgg
     2461 gatccccatg acgaagggct tcatcagccc cgtggcgacc acgacgccct ggtacccctg
     2521 gctcagcagc gcgggcacat gagaaacttt cgtcccgagc ttgagctcga tgaggccgag
     2581 gctcagcagc cactcgatgt cgctctggac cacgtcccgg ggcagccggt gctcggggat
     2641 ccagttgcag gtcccacccg gctggctctc ctgatcgaag acggtcaccg cgtagccgag
     2701 ccgggcgagg tacgccgcgg cggccagccc cgcggggccg gccccgacga ccgcgacctg
     2761 cttgccgttg ggcgcgggtg cggccggccg cggcatgacg ccgagggcct tggccttggc
     2821 gatgagggtc gcctgcaccg aggggatctc cacggcccga tcgaaggtct tgtagacgca
     2881 ggccgcctt
//
LOCUS       DAOPWU010000411         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_35798_length_2885_cov_5.097173, whole genome shotgun sequence.
ACCESSION   DAOPWU010000411 DAOPWU010000000
VERSION     DAOPWU010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35798_length_2885_cov_5.097173"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PKM23_14425"
     CDS             complement(<1..1034)
                     /locus_tag="PKM23_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY92717.1"
                     /translation="MKKIFSFVLTMALLVALMSTMGCQTKEVTSAKVYISQNNWDKAI
                     EQLEQAVQIYPNDAEARYLLGEGYGNKGLWEKMNEMFNQSTALAPTFATQIKNTREKY
                     WVNVFNQGVAKVNAKEGETADLAGAAKFFSDAVRIDPSHIESYKNLAYAHLRSNNLPA
                     AIKAYQDYLAINPKEVSILNEVTRLYLENKEYVKAQETANTVLGVDAANIDAISNLAM
                     AYDLDGQNDKAILTYETALAKNPEDQDLLFNLARLNYLNKDYDKAIAMFQKVISLNPE
                     DYDANLNVGNAYLTMADDLRKSLVEKENVKQTVSEEDVNKLKEFYRAAIPFLEKAISI
                     KPENSAVYYNL"
     gene            complement(1101..2147)
                     /gene="mtnA"
                     /locus_tag="PKM23_14430"
     CDS             complement(1101..2147)
                     /gene="mtnA"
                     /locus_tag="PKM23_14430"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNY92718.1"
                     /translation="MDELQGISWHDGRVRILDQTRLPEELVALEISDYQGIIRAITGM
                     NVRGAPAIGIAAAYGIVLAVWSLEEADRPAFLQRVNQASEALARSRPTARNLFWALER
                     MRAALMKLLSRPLREIKLALLEEAQRLHADDIERCRAIGRFGAALLPQTTSIMTHCNA
                     GAFATGGFGTALGVIRTAVSQGKKVQVYACETRPLLQGSRITAWELAEEEIPVTVLCD
                     SMAAYAMARGLVQMVIVGADRIARNGDAANKIGTYGLAILARHHRIPFYVAAPLSTFD
                     LEIRSGDHITIEQRAAAEVELCGGMRAVPEGVPVLNPSFDVTPAKLIDAFITEKGIIR
                     PPFEESLTSLQVKA"
     gene            complement(2152..>2885)
                     /locus_tag="PKM23_14435"
     CDS             complement(2152..>2885)
                     /locus_tag="PKM23_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92719.1"
                     /translation="PDNAAHFHPIYPWPERIHVCGDTRYDQVSQRALETGKIEFLLES
                     GRFTRERCLVIGSSWPSDERVILPAVVEALARDPGFRVIIAPHETTEEHLRGIESHLA
                     GSGISFLRLGEFSPASPDFQVLLIDRIGLLANLYALGGLAFVGGGFGPGVHSVLEPAA
                     HGCAVCFGPHHVNSLEAAALLRRGGGVLIDSTAAMDRALQQFLSGGEEMRRIGGEAGA
                     LVRENLGASGRVVEAILRHLKSIKE"
BASE COUNT          513 a          825 c          941 g          606 t
ORIGIN      
        1 aggttgtaat agaccgcgga gttttccggc ttgatgctga tggctttttc cagaaagggg
       61 atggccgcgc ggtagaactc tttcagcttg ttgacatctt cctcgctgac agtctgcttg
      121 acgttctcct tctcgaccag gctcttgcgc aggtcgtcgg ccatggtcag ataggcattg
      181 ccaacgttga ggttggcatc gtaatcctcg ggattaaggc tgatgacctt ctggaacatg
      241 gcgatggcct tgtcatagtc cttgttgaga tagttcaggc gggccaggtt gaagagcagg
      301 tcctgatcct cgggattttt ggccagggca gtttcgtagg tcaggatcgc cttgtcgttc
      361 tgaccgtcga ggtcgtaggc catggcgaga ttggagatgg catcgatgtt ggcggcgtcg
      421 acgccgagca cagtgttggc ggtctcctgg gccttgacgt actccttgtt ctccaggtag
      481 aggcgggtca cctcattgag gatcgagacc tctttgggat tgatggcaag atagtcctga
      541 taggccttga tcgcggcggg aaggttgttg ctgcgcagat gggcgtaggc caggttcttg
      601 taggactcga tgtggctcgg atcgatgcgg acggcgtcgc tgaaaaactt ggcggcgccc
      661 gcaagatcgg cagtctcgcc ctctttggcg ttcaccttgg cgacgccctg attgaagaca
      721 ttcacccagt atttttcgcg ggtatttttg atctgcgtgg cgaaggtcgg agccagcgcg
      781 gtggactggt tgaacatctc gttcatcttt tcccagaggc ccttgttgcc atagccctca
      841 ccgaggaggt aacgcgcctc ggcgtcattg ggatagatct ggacagcctg ttcgagctgt
      901 tcgatggcct tgtcccagtt attctggctg atgtagacct tggccgaggt gacttccttg
      961 gtctggcagc ccatggtcga catcaaggcg accagcaggg ccatggtaag gacaaatgag
     1021 aagatctttt tcattgtttt cgctcccaag agtttggttt tggatttgat tcaataattt
     1081 aaaataaaaa atatttctaa tcaagctttt acttgcaaag aggtcaaact ttcctcaaat
     1141 ggcggcctga tgatcccctt ttctgtgata aaggcgtcga tgagcttggc gggggtgaca
     1201 tcaaaagagg ggttgagcac cggcacgccc tcggggactg ctcgcatgcc cccgcacagc
     1261 tccacctcgg cggcggcgcg ctgttcgatg gtgatgtgat cgccgctgcg gatctccagg
     1321 tcgaaggtgg agaggggggc ggccacataa aaggggatgc ggtgatggcg ggcgaggatg
     1381 gccagtccat aggtgccgat cttgttggcg gcgtcgccat tgcgggcgat gcggtcggcc
     1441 ccgacgatga ccatctgcac cagtccacgg gccatggcat aggcggccat gctgtcgcaa
     1501 agcaccgtca ccgggatctc ctcctcggcc agctcccagg cggtgatgcg gctgccctgc
     1561 agcaggggcc gggtctcgca ggcgtagacc tggactttct tgccctggct cacagccgtg
     1621 cggatcactc cgagcgccgt cccgaagccg ccggtcgcaa aggccccggc gttgcagtgg
     1681 gtcatgatcg atgtggtctg gggtagcagg gctgcgccga agcggccgat cgcccggcag
     1741 cgctcgatat cgtcggcgtg aagcctttgc gcctcctcga ggagggcgag tttgatctcg
     1801 cgcagggggc ggctgagcag cttcatgagg gcggcgcgca tccgttccag ggcccagaag
     1861 agattgcgcg ctgtcggccg gctgcgcgcc agggcctcac tcgcctggtt gacgcgctgc
     1921 agaaaggcgg gccggtcagc ctcctccagg gaccagaccg ccaggacaat accataggcg
     1981 gcggcgatgc cgatggcggg ggcgccgcgc acgttcatcc ccgtgatcgc gcggatgatg
     2041 ccctgataat ccgagatctc cagcgccacc agctcctccg ggagcctggt ctggtcgaga
     2101 atacgcaccc ggccgtcgtg ccaggagatg ccctgcagct catccattgc tttattcctt
     2161 tatgctcttc aggtgacgga ggatggcctc cacgacccgg ccgctggcgc cgagattctc
     2221 gcgcaccaga gcgccggcct ccccgccgat tctgcgcatc tcctcccccc cgctcaaaaa
     2281 ctgctgcaag gctctgtcca tggcagccgt ggagtcgatc aggactcccc cgccgcgccg
     2341 gagcagggcc gccgcttcaa gtgaattgac gtgatgcggc ccgaagcaga cggcgcagcc
     2401 atgggccgcc ggttcgagga cgctgtgcac tccgggccca aagcccccgc cgacgaaagc
     2461 caggccgccc agggcgtaga gattggcgag caacccgatg cggtcgatga gcagcacctg
     2521 aaagtcaggg ctggcggggc tgaattcgcc cagtcttaga aagctgattc cggacccggc
     2581 gaggtggctc tcaatcccgc gcagatgctc ttcggtggtc tcgtggggcg cgatgatgac
     2641 cctgaagccg ggatcccggg ccaaagcctc gaccaccgcg gggaggatca ccctctcatc
     2701 agaaggccag gagctgccga tcaccaggca gcgctcgcgg gtgaagcggc cgctttccag
     2761 gagaaactcg atcttgccgg tctcgagggc gcgttggctg acctggtcgt aacgggtgtc
     2821 tccgcaaaca tggatgcgct cgggccaggg atagataggg tggaaatgag ctgcgttatc
     2881 ggggg
//
LOCUS       DAOPWU010000412         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_36072_length_2870_cov_5.173712, whole genome shotgun sequence.
ACCESSION   DAOPWU010000412 DAOPWU010000000
VERSION     DAOPWU010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36072_length_2870_cov_5.173712"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2242)
                     /locus_tag="PKM23_14440"
     CDS             complement(<1..2242)
                     /locus_tag="PKM23_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNY92720.1"
                     /translation="MKRLFFSSLVSVMFVILFSGFSGLSAQSLAEIEAGRVALPNGWK
                     LTPVGKKVPLGDLPLNLTLSASQRWLAVTNNGQSDQSIQLYDIRKNSMADSVAVGKAW
                     LGLAFSDDEKFLYASGGNDNWIVKFALAGGRLKAVDTLVLGKPWPALISVAGIALDDS
                     RHLLYAVTRENNSLYTVDTGSGQVLARYPLGGEAYTCLLSPDRSRLYISCWGCNQVVL
                     FDAVTQQLDGQVPVGDNPNDLCLSRNGEWLFVANANDNTVSVINTRLRKVVETLNTAL
                     FPDAPSGSTANSLALSGDDRSLYVANADNNCLAVFDVEEPGTSISRGFIPTGWYPTCV
                     RAAGGKLYIANGKGLSSLANPRGPNPAGKRADVGYQQGSRQKEQYIGGLFRGVLSILA
                     EPDDALLGVYSRAVYTNTPYTKNRETSSEGEAGNPIPMRVGDPSPIRYVFYVIKENRT
                     YDQILGDLPEGNGDTTLVLFGARITPNHHALAREFVLLDNFYVNGEVSADGHNWSLGA
                     YATDYLEKSWPTTYGGRGGFYTAEGRREIANNRDGFIWDFCKRYGVSYRTYGEFADDY
                     KPNIPVLEGHLCPYFTCWDQSVRDTTRFYQWKRDFDSLYAAGSVPQLNTIRFINDHTE
                     GLGVGKPTPFAHVADNDLTIGLFVDYLSHHPIWNQSAIFIVEDDAQNGPDHVDAHRTV
                     ALVAGGFVKSAFVDHTLYSTSSMLRTIELILGLPPMSQYDAAATPMWRCFAQTTGHAP
                     YAVRPIN"
     gene            2574..>2870
                     /locus_tag="PKM23_14445"
     CDS             2574..>2870
                     /locus_tag="PKM23_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92721.1"
                     /translation="MKKNLSFSDSRAWSTDLVRLGWLFLLAILFLIMFSGAGYGQASI
                     SGSAWMDQNKDGLRGESERAFSGVRVQLFRRTSGAAYEVLTSLRTQSDGSFLFLV"
BASE COUNT          589 a          786 c          907 g          588 t
ORIGIN      
        1 cattgatcgg ccgcacggca taaggggcgt ggcctgtggt ctgggcgaaa cagcgccaca
       61 tcggcgtcgc cgcggcatca tactggctca tcggcggcag gccgaggatg agctcgatgg
      121 tgcgcagcat cgaggaggtg gaataaagag tgtggtcaac aaaagcgctc ttgacgaatc
      181 ctccggccac gagggcgacc gtgcggtggg catcgacatg gtcaggaccg ttctgggcat
      241 catcctcgac aataaaaatg gcgctctggt tccagatcgg atggtggctg agatagtcga
      301 cgaagaggcc gatggtcagg tcattgtcgg cgacatgagc gaagggagtg ggtttgccga
      361 cgccgagccc ctcggtatgg tcattgatga agcggatggt gttgagctgc ggaacgctgc
      421 cggcggcata gagggaatca aagtcccttt tccactgata gaagcgggtg gtgtcgcgca
      481 cgctctggtc ccagcaggtg aaatagggac agaggtggcc ttcaagaacc gggatgttgg
      541 gcttgtagtc atcggcgaac tcgccgtagg tgcgatagct gaccccataa cgcttgcaga
      601 aatcccagat aaaaccatcg cggttattgg cgatttcccg cctcccttcg gcggtgtaaa
      661 agccgccgcg gccgccataa gtcgtaggcc agctcttctc gagatagtcg gtggcgtagg
      721 cccccaggct ccagttgtgg ccgtcggcgc tcacctcgcc gttgacataa aaattgtcga
      781 ggaggacaaa ttcacgcgcc agggcgtgat ggttaggggt gatccttgca ccgaagagga
      841 ccagagtggt gtcgccattg ccctcgggca gatcgcccag tatctggtca taggtccgat
      901 tttccttgat cacgtaaaaa acataacgga tgggcgaggg atcgccgacg cgcatgggga
      961 tgggattgcc cgcctccccc tcactgctgg tttctctgtt cttggtgtag ggcgtattgg
     1021 tgtagacggc ccgcgaatag accccgagca gcgcgtcatc aggctccgca aggatgctga
     1081 gtacaccgcg gaagaggccg ccgatgtact gctccttttg ccggctgccc tgctgataac
     1141 cgacatcggc gcgcttgcct gcgggattgg ggccccgggg attggccaga gaggagaggc
     1201 ccttgccgtt ggcaatataa agcttgcccc cggccgcgcg cacacaagtc gggtaccagc
     1261 cggtggggat aaagcccctg ctgatgcttg tgccgggctc ctcgacatca aagaccgcca
     1321 ggcagttgtt gtccgcattg gcgacataga ggctcctgtc gtccccggag agggcgaggc
     1381 tgttggcggt cgatccgctc ggggcgtcgg ggaagagggc ggtgttgagg gtctccacca
     1441 ccttgcgcag ccgcgtgttg atgaccgaga cggtattgtc attggcattg gctacaaaaa
     1501 gccactcgcc gttcctgctc aggcagaggt cgttgggatt gtcgccgaca ggaacctggc
     1561 cgtcgagctg ctgggtgacg gcatcgaaga gcaccacctg gttgcacccc cagcaggata
     1621 tatagagcct gctgcggtcc ggcgagagca ggcaggtgta cgcctcgcct ccgaggggat
     1681 agcgggcgag cacttgaccg ctcccggtat ccacggtgta aagggaattg ttctctctgg
     1741 tcacggcata aaggagatgg cgcgagtcat cgagggcgat accggccaca gagatcaggg
     1801 ccggccaggg cttgcctagc acgagggtat ccaccgcctt gagcctgccg cccgcaagcg
     1861 cgaactttac tatccagttg tcgttgccgc cggaggcgta gaggaatttt tcatcgtccg
     1921 aaaaggccag gcccaaccag gccttgccga cagcgacaga gtcggccatg ctgttcttgc
     1981 ggatgtcata gagctgaatc gactggtcgc tctggccgtt gttggtgacc gccagccagc
     2041 gctgcgaggc cgagagggtg agattgaggg gcagatcacc gagggggacc tttttaccga
     2101 ccggcgtcag cttccagcca ttgggcaggg caacccgccc ggcttcgatt tccgccaggc
     2161 tctgcgctga taaccccgaa aagccgctga aaaggataac aaacataacg gagacaagcg
     2221 aagagaagaa aagccttttc attggatcgc tcctcatcct gtggatgccg ctctgtccgg
     2281 ccgagctggt tacggccgga ccgggattga tttatgtcta ttttatcact cttctgaata
     2341 aaaaacaagt attaccttgc tgccgaaaag ccgcaattct ggcacacctt ttgactaaga
     2401 gagtatgaaa tcaggccgcg cacgcctggc ggctgattaa taatttattt aataacagtt
     2461 agttgaaata tcctgaacct cgcagatacg atccgattat gtggcgccgc ttcctcatca
     2521 gggatattcg atgtggcatg aatggggcat tgagcaatcc gaggagaaag attgtgaaaa
     2581 agaacctgag cttttctgat tcccgcgcct ggagcaccga tctcgtccgg ttgggctggc
     2641 tctttctgct ggcgatcctt ttcctgatca tgttcagtgg agcgggctac ggccaggcct
     2701 ccatcagcgg cagcgcctgg atggaccaga acaaggacgg cctccggggc gaaagcgaac
     2761 gcgccttctc cggggtgcgc gtgcagcttt tccggcgtac aagcggggcc gcctatgaag
     2821 tcctcacctc cctgcgcacc cagagtgatg ggtcattcct ctttctggtc
//
LOCUS       DAOPWU010000413         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_36243_length_2861_cov_4.582680, whole genome shotgun sequence.
ACCESSION   DAOPWU010000413 DAOPWU010000000
VERSION     DAOPWU010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36243_length_2861_cov_4.582680"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PKM23_14450"
     CDS             <1..838
                     /locus_tag="PKM23_14450"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HNY92722.1"
                     /translation="HFNALMDVDKHYRRKEYLLFPLLEKKGITGPPTVMWGKHDEARG
                     MLKAALEALHTLTGADQASLTGAIDLLLHPAADAIEEMIYKEEEILFPMCLDTLTEAE
                     WAAILAGSADIGYCLYAPTAVWTPSEEAAPASARPEEAGPSGRITLPTGSFSVEELTA
                     VLDNLPFDITFVDAEDTVRYYSAGSERIFERNLAVIGRKVQLCHPPSSMHIVQKILDD
                     FRAGRQNRAPFWITMGDKFIHIEYFALRDAAGKYLGTLEVTQDLTAKKKLSGNQRLLS
                     YS"
     gene            931..1440
                     /locus_tag="PKM23_14455"
     CDS             931..1440
                     /locus_tag="PKM23_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNY92723.1"
                     /translation="MELTITPETRLSALLEAYPQLEEKLLSMSPQFAKLRNPVLRATI
                     AKIATLRQVAEVGGLPLAEVINTLRAAVGQMCGNYGGPAESDDRPAWFAADKITRRLD
                     ARPLLAAGEHPLTQVVSESQQLQPGEIYELTTPFLPAPLIDAVKKKGFAAWSLQEQAD
                     LYRSYFCKE"
     gene            1491..2255
                     /locus_tag="PKM23_14460"
     CDS             1491..2255
                     /locus_tag="PKM23_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY92724.1"
                     /translation="MNSDNHEAAEFYRGLAASYDGMTRFAERLPGERALLAEWRTRHG
                     FTRPLDAACGTGLHAVALAQLGLEVTGADLSPEMLAGARAHAAAEGVEVTWVEAAMER
                     LPAHVQGPFDALFCLGNSLPHLLDEEALAAALAGFHQLLVPGGLLLLQLVNYEAVLAG
                     QRRILALNRDGEREFIRFYDFGESLLGFNILEIDWSQQPPRHRLQTTPLRPWRHAEVE
                     RAVEEAGFGNIDIFGDLRRGEYATAKSPNLVVTAIR"
     gene            complement(2349..2852)
                     /locus_tag="PKM23_14465"
     CDS             complement(2349..2852)
                     /locus_tag="PKM23_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HNY92725.1"
                     /translation="MLVAAYLVSVVAANLVIAAFGPRATAIVAFLLIGLDLTSRDALH
                     EAWRKRSLLPKMTLLIFAGSLISWLINREAAAVAVASFAAFAAAGAVDALSYHLLRDR
                     LWLVKVNGSNIPSALIDSLLFPTLAFGAVMPAIIAGQFLAKVAGGFFWSLVLHHLFRR
                     PQTAPGA"
BASE COUNT          523 a          943 c          935 g          460 t
ORIGIN      
        1 ccatttcaac gccctgatgg atgtggacaa gcactaccgc cgcaaggagt acctcctctt
       61 tccgctgctg gagaagaagg ggatcaccgg tccgccgacg gtgatgtggg gcaagcacga
      121 cgaggcgcgc gggatgctca aggccgccct cgaggccctg cacaccctga ccggggccga
      181 ccaggcctcc ctcaccggcg ccatcgacct gctgctgcat cccgccgccg acgccatcga
      241 ggagatgatt tacaaggagg aggagatcct cttcccgatg tgcctggaca ccctcaccga
      301 agcggagtgg gcggccatcc tcgccggctc agccgatatc ggctactgtc tctatgcccc
      361 gaccgcggtc tggaccccct cggaagaggc cgcccccgct tcggcgcggc ccgaagaggc
      421 cggcccctcc ggccgcatca cgctcccgac cggcagcttc agcgtcgagg agctcaccgc
      481 ggtgctcgat aatcttcctt tcgatatcac ctttgtcgat gcggaggaca cagtgcgcta
      541 ttactccgcg ggcagcgagc gcatctttga gcgcaacctc gccgtcatcg gccgcaaggt
      601 gcagctctgc catcccccct cctccatgca tattgtccag aagattctcg acgactttcg
      661 cgccggccgg cagaaccgcg cccccttctg gatcacgatg ggcgacaaat tcatccacat
      721 cgagtacttc gccctgcgcg acgccgcagg aaagtacctc ggcaccctcg aggtgaccca
      781 ggatctcacc gcaaagaaaa agctgagcgg caatcagcgc ctgctttcct acagctgaca
      841 gagcgcgggc cggtgcacgc cggtccgccg gattcgacag atgagagggc ggccgttctg
      901 ccgccccttt ttttaaaaaa ggagaaaatc atggagttga ccatcacccc cgagaccagg
      961 ctgagcgccc tgctcgaggc ctatccccag ctcgaagaga agctgctttc gatgtcgccc
     1021 cagttcgcca agctgcgcaa tccggtcctg cgcgccacca tcgccaaaat cgccaccctg
     1081 cgccaggtgg cggaggtcgg cggtctgcct ctggccgaag tgatcaacac cctgcgcgcg
     1141 gcggtcggcc agatgtgcgg caattatggc gggcccgccg aatctgacga tcgtcccgcc
     1201 tggttcgcag cggacaagat cacccggcgt ctggacgccc gaccgctgct ggcggccggc
     1261 gagcatcccc tgacgcaggt ggtgagcgag agtcagcagc tgcagccggg cgaaatctac
     1321 gaactcacca ccccctttct gcccgcaccg ctgatcgatg cggtcaaaaa gaagggtttt
     1381 gccgcctgga gcctgcagga gcaggccgat ctctaccgca gttatttctg caaggagtaa
     1441 taagggaaaa ccgaacgaaa gggcggccgc gatggccgga gggaaaaacg atgaattccg
     1501 acaaccatga agcggcggag ttttaccgcg gcctggccgc cagctacgac ggcatgaccc
     1561 gtttcgccga gcggctgccc ggcgagcggg cgctgctggc cgaatggcgg acgcgccacg
     1621 gcttcacccg tcccctcgat gcggcctgcg ggaccgggct gcatgcggtc gccctggcgc
     1681 agctgggcct cgaggtgacc ggggccgatc tctcgccgga gatgctcgcc ggggcgcgcg
     1741 ctcatgccgc ggccgagggg gtcgaggtca cctgggtgga agcggcgatg gagcggctgc
     1801 ccgcgcatgt gcaggggccc ttcgatgccc tcttctgcct gggcaactct ctgccccacc
     1861 tcctggatga ggaggcgctg gcggccgccc tggcgggatt tcaccagctg ctcgtccccg
     1921 gcggcctgct gctgcttcag ctggtcaact acgaggcggt cctggccggg cagcgccgca
     1981 tcctcgccct caaccgcgac ggcgagcgtg agttcatccg cttttacgat ttcggcgaat
     2041 ccctgctggg cttcaacatc ctcgagattg actggtccca gcagccaccc cgccaccgcc
     2101 tgcagaccac tcccctccgc ccctggcgcc acgccgaggt cgagcgggcg gtagaagaag
     2161 ccggatttgg caacatcgac atttttggcg atctgcgccg gggagagtat gcgacggcga
     2221 aatcaccaaa tctggtggtg acggcgatca gatagcgccg gggctgccag cagggcgcat
     2281 ccgggatgaa aaaagccggt gcagataaat tctgcaccgg cttttttttc ggatctgcct
     2341 cgcgcggatc aggcgcccgg ggcggtctgc ggcctgcgga aaagatggtg gaggacgagg
     2401 gaccagaaaa agcccccggc caccttggcg aggaactgtc ctgcgatgat ggcgggcatg
     2461 accgcgccga aggccagggt cgggaagagc aggctgtcga tgagggcgct ggggatgttg
     2521 ctgccgttga ccttgaccag ccagaggcgg tcgcgcagga ggtggtagct gagggcgtcg
     2581 acagccccgg ccgcagcaaa agcggcaaat gaggcgacgg caacggcggc ggcctcccgg
     2641 ttgatgagcc aggagatgag gctgccggcg aagatgagca gggtcatctt gggcagcagc
     2701 gagcgcttgc gccaggcctc gtgcagggcg tcgcggctgg tcagatcgag gccgatgagc
     2761 agaaaggcga cgatggccgt ggcccggggt ccaaaggcgg cgatgaccag attggccgcc
     2821 accacgctga ccaggtaggc cgcgaccagc aggctgtttt t
//
LOCUS       DAOPWU010000414         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_36284_length_2859_cov_4.683310, whole genome shotgun sequence.
ACCESSION   DAOPWU010000414 DAOPWU010000000
VERSION     DAOPWU010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36284_length_2859_cov_4.683310"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PKM23_14470"
     CDS             <1..719
                     /locus_tag="PKM23_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNY92726.1"
                     /translation="RPLHYGSGLRRHTRLLLATDIHLGTLVHNSRMQELVSLMQAQNP
                     DLILFGGDLFDEDILSLSRQNMAEVLRQLQPPHGVYAIMGNHEHISGSKEAVRYMEES
                     GITVLRDRAVRVAEAFWLIGREDRQAVFMGSGRQPLAEIMAGIDRREPLILLDHQPFH
                     LEEAVKEGIDLQLSGHTHHGQFFPFNLITGAMYEMSWGYLRKGSSHFYVSCGAGTWGP
                     PVRLGNRPEIVVIDLTFNKK"
     gene            736..1677
                     /locus_tag="PKM23_14475"
     CDS             736..1677
                     /locus_tag="PKM23_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY92727.1"
                     /translation="MSDLYQAYRSCTLCPRECRVDRTSGRLGICGESAELRIASIEAH
                     FGEEPPISGTHGSGTLFFSGCTLLCSFCQNYQISCFHIGDRTTVEKAADQVADLVQHR
                     GIHNLNLVTPDHFLPHAIDLIQRLRTRGIALPVVFNSSGFEKVELLRQTEEYADIYLP
                     DFKYSDAELAHALSHCRSYPLVALDAIAEMVKQKGFLDHFSEGRAIASRGVLVRHLVL
                     PGQTANSIEALSMLFAEFGPELPLSLMSQYRPARMVKQAELNRPLRPEEFSRVYDHLL
                     ELGFKNCFVQHMEGSPEEEDPFLPDFNRERPFKGNLR"
     gene            complement(1723..>2859)
                     /locus_tag="PKM23_14480"
     CDS             complement(1723..>2859)
                     /locus_tag="PKM23_14480"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY92728.1"
                     /translation="TALTDARPAVEALKSGAYDYLLKPFELDELKLVVAKALETHRLK
                     LEVARLKEQQRRRAPESGLYGESAAIQEVLNLVKIIAETPRTSVLLEGESGTGKELVA
                     DAIHAASARAEKPMIKINCAAIPENLLESELFGYEKGAFTDAKGTKRGLFELAHGGTI
                     FLDEISSMKISLQPKLLRVLESSTFRRIGGTSDITIDVRIIAATNQNLEACVRDGLFR
                     EDLLYRLKVMVIMLPALREHPEDILPMAKLFIEQNNREFNRSIKGISAEAAEMMLRYT
                     WPGNVRELKNVLERAVILCKREEITPELLHLEPVEMPAAAPAPPAARTPGTVNADGET
                     LAEIERQHVLRTLEKYHNNKSRTAKALNISRSTLREKLKEYGLE"
BASE COUNT          497 a          944 c          857 g          561 t
ORIGIN      
        1 atcgtcctct ccactacggg agcggtctac gaaggcatac gcgcctgctc ctcgccaccg
       61 acatccacct tggtaccctg gtccataact cgcgcatgca ggagctggtc agcctcatgc
      121 aggcgcaaaa tcccgatctc atcctcttcg ggggcgacct tttcgacgag gacatcctct
      181 cgctctcgcg gcagaacatg gccgaggtgc tccgtcagct ccagccgccc cacggcgtct
      241 atgccatcat gggcaaccat gaacacatca gcggcagcaa ggaggccgtc cgctacatgg
      301 aggagtccgg gattaccgtg ctgcgtgacc gcgcggtgcg ggtggcggag gccttctggc
      361 tcatcggccg ggaggaccga caagccgtct ttatgggctc cgggcggcaa cccctggcgg
      421 aaatcatggc ggggatcgac cgccgcgagc ccctcatcct gctcgatcat cagcccttcc
      481 atctcgagga ggctgtgaaa gagggcatcg atctgcagct ctctggccat acccatcacg
      541 gccagttctt ccccttcaac ctgatcaccg gggcgatgta cgagatgagc tggggctatc
      601 tgcgcaaggg cagcagccac ttttatgtct catgcggcgc cggcacctgg ggaccgccgg
      661 tccggctcgg caaccgcccc gagatcgtcg tcatcgacct caccttcaac aaaaagtgat
      721 tgggcaggcg tccgcatgag cgatctctat caggcctaca ggtcatgcac cctctgcccg
      781 cgcgagtgcc gggtcgaccg gacgtccggc aggctgggga tctgcgggga atcggccgaa
      841 ctgcgcatcg cctccatcga agcccatttc ggtgaagagc ccccgatcag cggcacccac
      901 ggttcgggca ccctcttttt cagcggctgc accctgctct gcagtttttg ccagaattac
      961 cagatctcct gcttccacat cggcgaccgg acgacagtgg aaaaggcggc cgatcaggtc
     1021 gccgatctgg tacagcaccg ggggatccac aacctcaatc tggtcacgcc ggaccatttc
     1081 ctgccgcacg ccatcgacct catccagcgc ctgcgcaccc ggggaatcgc cctccccgtc
     1141 gttttcaaca gctcgggatt tgaaaaggtg gagctcctgc gccagacgga ggagtatgcc
     1201 gacatctatc tgccggattt caaatacagc gacgcggagc tggcgcacgc gctctcgcac
     1261 tgccgctcct atcccctggt cgcactcgat gccattgcgg agatggtgaa acagaaggga
     1321 tttcttgacc acttttccga gggacgggcg attgcgagcc ggggcgtgct ggtccgccac
     1381 ctcgtcctgc ccggccagac cgccaactcg atcgaggccc tctcgatgct ctttgcagaa
     1441 ttcggcccgg aacttcccct gagcctgatg tcgcagtacc ggcccgcccg tatggtcaaa
     1501 caggcggagc tcaatcgccc cctccggccc gaggagttca gtcgcgtcta cgaccacctc
     1561 ctcgaactcg gctttaaaaa ctgcttcgtt cagcacatgg agggctcccc cgaggaggag
     1621 gatccctttc ttccagattt caaccgcgaa cgccccttca agggcaatct gcgctgatcc
     1681 agcccccgcc ggccggccgc cccaagcccc tccgcctgcc gctcactcca acccgtactc
     1741 cttgagcttc tcccgaagtg tcgagcgcga gatattgagg gccttggcgg tcctggactt
     1801 gttgttgtgg tatttctcca gggtgcgcag aacatgctgg cgctcgatct ccgccagggt
     1861 ctccccatcg gcattgaccg tgccgggtgt gcgtgctgcc gggggagccg gcgccgccgc
     1921 cggcatctcg accggctcga gatgaaggag ttcgggcgtg atttcctccc gcttgcagag
     1981 aatcaccgcg cgttcgagga cgttcttgag ctcgcgcaca ttgcccggcc aggtatagcg
     2041 cagcatcatt tccgccgcct cggccgaaat acccttgatc gaccggttga attcgcggtt
     2101 gttctgctcg atgaagagct tggccatcgg caggatatcc tccggatgct cgcgcagggc
     2161 gggcaacatg atgaccatca ccttgagccg gtagagcaga tcctcgcgga agagtccgtc
     2221 gcgtacacag gcttccagat tctggttggt ggcggcgatg atgcgcacat cgatggtgat
     2281 gtcggaggtg ccgccgatgc gccggaaggt gctgctttcc agcacccgca ggagtttggg
     2341 ctgcagggag atcttcatgc tcgaaatctc gtccagaaag atggtgccgc cgtgggccag
     2401 ctcaaagagg ccgcgcttgg tccccttggc gtcggtgaaa gccccctttt catagccgaa
     2461 aagttcgctc tccagcaggt tctccgggat ggcggcgcag ttgatcttga tcatcggctt
     2521 ctcggcgcgg gcgctggccg catggatggc atcggcaacc agctccttgc cggtgccgct
     2581 ctccccctcc agcagtaccg aggtgcgcgg ggtctcggcg atgatcttga ccagattgag
     2641 cacctcctgg atggcggcgc tctcaccata gaggcccgat tccggcgccc ggcggcgctg
     2701 ctgctccttg aggcgggcga cctccagctt gaggcgatgg gtctcgaggg ccttggccac
     2761 caccagtttg agttcgtcca gctcgaaggg tttgagcaga taatcatagg cccccgattt
     2821 gagagcctcc actgcgggac gggcgtcggt gagggcggt
//
LOCUS       DAOPWU010000415         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_36711_length_2834_cov_4.267362, whole genome shotgun sequence.
ACCESSION   DAOPWU010000415 DAOPWU010000000
VERSION     DAOPWU010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36711_length_2834_cov_4.267362"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKM23_14485"
     CDS             <1..578
                     /locus_tag="PKM23_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNY92729.1"
                     /translation="FRALLEKYGRLSCIQLDAHSDLREEYEGSPLSHASVMARIREMT
                     PHALQLGIRAQCIEEAQAIAAHNWPVCTMHDYRKRRFDVDAALAALPDPVFVTVDLDA
                     FDLSVIHSTGTPEPGGFTWDEGLELLEKIFMRKNVVGFDLVELSYDEADRNSAFCAAR
                     LAYKMLGFKLAAGVARGLLTWPEKPAGPLFK"
     gene            complement(705..1640)
                     /locus_tag="PKM23_14490"
     CDS             complement(705..1640)
                     /locus_tag="PKM23_14490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNY92730.1"
                     /translation="MDKKPVLILFIFILLAIARPGVTQEERPGAWMDYVAPTYTILPN
                     LTYATASNMELKLDLYLPLERSKPVPVVVYFHGGGWVEGMKERNTLYLLPWLSMGWAA
                     VNVEYRLARHAPAPAAVEDGRCALRWLALHAGEYSLDPNRIVLTGHSAGGHLALITAL
                     LPEGSPFDRQCPSAESHRWVDGVEPPVKAAAVINWYGISDVNDVLEGINAKHYTIEWF
                     GSLPNREELARQLSPLTHVAAGAPAVLTIHGDADKVVPYTQAQRLHAALDKAGVPNRL
                     VTVSGGGHGGFSRQDLLGCYAAIREFLRKNGIVQE"
     gene            complement(1640..2815)
                     /locus_tag="PKM23_14495"
     CDS             complement(1640..2815)
                     /locus_tag="PKM23_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNY92731.1"
                     /translation="MQNGLFLPLLFFWAAGLVFTASASAAERPRARDLGIVVGALEPG
                     PLNAITDVPGVRVGQTTLLRGDSVRTGVTVVLPHGGNLYESRVPAAIAVGNGYGKLTG
                     STQVAELGELESPVALTNTLAVWGVAEGLARWMLGQPGMEKVRSINPVVAETNDGWAL
                     NAIRSFPVSFADLTAALESASTGAVAEGAVGAGTGTVAFEWKGGIGTSSRLIPDKPWH
                     VGVLVQSNFGRWQDLTIAGVPVGRALGRSGVHPHTNKGADGSIVVVVATDAPVSDRNL
                     QRLAARALLGIARTGGFASNGSGDYVIAFSTHPALRRTPAGPQPEPAAGPEVGNSQMN
                     PLFEAAVDATVEAIYNSLLRATTQSGAYATVEALPIDEVKAILARHGIGSAAGKEKK"
BASE COUNT          497 a          952 c          912 g          473 t
ORIGIN      
        1 tcttcagggc cctgctcgaa aagtacggcc gcctctcctg catccagctc gacgcccatt
       61 ccgacctgcg cgaagagtac gagggcagcc cgctcagcca cgccagcgtc atggcacgca
      121 ttcgcgagat gaccccccat gccctgcagc tcggcatccg cgcccagtgc atcgaggagg
      181 cgcaggccat cgccgcccac aactggccgg tgtgcaccat gcacgactac cgcaagcggc
      241 gctttgacgt cgacgccgcc ctggcggcct tgcccgatcc ggtcttcgtc actgtggacc
      301 tcgacgcctt cgatctgagc gtgatccaca gcaccggcac acccgagccg gggggcttta
      361 cctgggatga ggggctggaa ctgctggaaa agattttcat gcggaaaaat gtggtgggat
      421 tcgacctcgt cgagctctcc tatgacgagg cggaccgcaa ttcggccttt tgtgccgccc
      481 gtctggccta caagatgctc ggcttcaagc tcgccgccgg ggtggcgcgc gggcttctga
      541 cctggccgga gaagccggcc ggccccctct tcaaatagca gatctcgcca gggacggccg
      601 ctgacggcgg ccgtctgtac ttcctcccca tccggctacc tctctatccc cttcacctgc
      661 agaaacagcc ggccactgat gcagtcgttg atgactttcc ggcgctactc ctgtacgatc
      721 ccgttcttgc gcagaaattc gcggatggcc gcatagcagc cgagcaggtc ctgccggcta
      781 aagccgccgt gcccgccgcc gctgaccgtc accaaccggt tgggcacgcc cgccttgtcg
      841 agggcggcgt gcaggcgctg cgcctgggtg taaggcacca ccttgtcggc atcgccgtgg
      901 atagtgagca cagccggggc tccggccgcc acatgggtca ggggcgagag ctggcgagcc
      961 aactcctcgc ggttgggcag gctgccgaac cattcgatcg tatagtgttt ggcgttgatc
     1021 ccctcgagca catcgttgac atcactgatg ccgtaccagt tgatcaccgc ggcggccttg
     1081 accggcggct caaccccgtc cacccagcgg tggctctccg ccgaggggca ctgccggtcg
     1141 aagggggagc cctcgggcag cagggcggtg atcagcgcga gatgtccgcc cgccgaatgg
     1201 ccggttaaga caatgcggtt gggatcaagg gaatattcgc cggcatgcag cgccagccag
     1261 cgcagggcgc agcggccgtc ctcgaccgca gccggggcgg gcgcatggcg cgccagccgg
     1321 tattcgacgt tgaccgccgc ccaacccatc gagagccagg gcaagaggta aagcgtattg
     1381 cgctccttca tcccctcgac ccagccgccg ccgtgaaagt agaccaccac tggcaccggc
     1441 ttgctccgct ccagcggcag atagaggtca agcttgagct ccatgttgct ggcggtggca
     1501 taggtgaggt tggggaggat agtgtaggtt ggggcgacat aatccatcca ggcgccgggg
     1561 cgctcctcct gggttacgcc cgggcgggcg atcgccagca ggatgaagat gaagaggatc
     1621 aaaacaggct tcttgtccat tatttcttct cctttccggc agcgctgccg atcccgtggc
     1681 gggccaggat cgccttgacc tcgtcgatcg gcagggcttc gacagtggca taggctcccg
     1741 actgggtggt ggcgcgcagc aaagagttgt agatcgcctc aacggtggca tcaacggccg
     1801 cttcaaaaag gggattcatc tggctgttgc cgacttccgg cccggctgca ggctccggct
     1861 gcggacccgc aggagtgcgg cggagggcgg gatgagtcga gaaggcgatg acataatcgc
     1921 ccgatccgtt ggaggcgaag ccgccggtgc gggcgatgcc gagcagggcg cgggcggcca
     1981 ggcgctgcaa attgcggtcg gagacaggag catcggtggc aaccacaacg acaatcgagc
     2041 cgtccgctcc cttattggtg tggggatgaa ccccgctgcg cccgagtgcg cggcccaccg
     2101 gcaccccggc gatagtcaga tcctgccagc gcccgaaatt ggactgtacc agcactccaa
     2161 catgccatgg cttgtccgga attaggcgcg aactggtgcc gatgccgccc ttccactcaa
     2221 aggccactgt gccggttccg gccccgaccg cgccctcggc caccgcccct gtcgaggcgc
     2281 tctccagggc agcggtcaga tccgcgaaag agactggaaa ggagcggatc gcattgagcg
     2341 cccagccatc gttggtctcg gcgaccaccg gattgatcga gcgcaccttc tccattccgg
     2401 gctggccgag catccagcgg gcgagcccct ccgccacccc ccagaccgcg agggtattgg
     2461 tcagggcgac cgggctttcc agttcgccga gctcggcgac ctgtgtcgat ccggtcagct
     2521 tgccgtagcc gttgccgacc gctatcgccg cgggcacacg gctctcgtag agattgccac
     2581 catgcggcag gaccactgtc accccggtgc gcaccgaatc cccgcgcagg agggtggtct
     2641 ggccaacccg caccccgggc acatcggtga tggcattgag ggggccgggc tcaagcgcgc
     2701 cgacgacaat gcccagatcg cgcgcccgcg gccgctccgc cgcacttgcg gaggcggtga
     2761 aaaccagacc ggcagcccag aaaaagagca gcggcagaaa cagaccattt tgcattcccg
     2821 atctcccgtt gttc
//
LOCUS       DAOPWU010000416         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_36784_length_2831_cov_3.641571, whole genome shotgun sequence.
ACCESSION   DAOPWU010000416 DAOPWU010000000
VERSION     DAOPWU010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36784_length_2831_cov_3.641571"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKM23_14500"
     CDS             <1..692
                     /locus_tag="PKM23_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92732.1"
                     /translation="TPEEVAHTVVMEIQGFATGYEVITALDSTVMDPSYRAGVLRQPL
                     IAEMQRLSDEKQTPSVALGQLGPPELSKLLYEAWLLRTKFRSMKKVLSADPALISHEL
                     ENYLLHHPIRHTIVSIGIPILLADGTTLLRGPQIKVPAGRVKDELNVTPETIDAWARK
                     GWVDLRPANMAKWQERIEAMFDSCKNFFNVDASDISRGPCPAETLRIGEMVGWVFNND
                     PAIQGFRVKSF"
     gene            714..1451
                     /locus_tag="PKM23_14505"
     CDS             714..1451
                     /locus_tag="PKM23_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY92733.1"
                     /translation="MGSAPAENPDPRRVQDSPDSSGENERILITLPVFNEALHLGQLI
                     TDLRKVTDLDILVIDDGSEDDTPAVLQRVGASCLRHNRNYGKGEALRSALKAARAWGY
                     HWIITMDGDGQHDPAALPVFIRTIRSHQADFYIGWRQGRGGIMPPLRQISNGISSVLL
                     SLFAGVRFHDAQCGFRAYRTAIPGLDACREQGFQYESELLLRLGRLDLRVDEIPIATR
                     YIGSSSRIRYLRDTLAFLRLLWRSLWW"
     gene            1546..1620
                     /locus_tag="PKM23_14510"
     tRNA            1546..1620
                     /locus_tag="PKM23_14510"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1581..1583,aa:Pro,seq:cgg)
     gene            1758..>2831
                     /locus_tag="PKM23_14515"
     CDS             1758..>2831
                     /locus_tag="PKM23_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNY92734.1"
                     /translation="MSSDYTFTEIEHKWQARWRESGLYRTDLNETAKKLYCLVMFLYP
                     SGDRLHIGHWYNYAPTDTWARFKRMQGFNVFEPMGYDAFGLPAENYAIKKGVHPAVST
                     AENIANIRKQLEAMGAMYDWDHEVNTSHPDYYRWTQWLFLKMYEHGLAFRKQQAANWC
                     PGCKTVLANEQVIDGHCERCESEVLSKDLKQWFFKTTAYSERLLEGHRTIDWPEKTLL
                     MQKNWIGKSVGATIDFKLEGREENIQVFTTRPDTLWGVTYMVLAPEHPLVERLTTPEH
                     KGAVAEYVLQARKQREIDRLSTEKEKNGVFIGSYCTNPVNQERVPIWIADYVLVTYGT
                     GAVMAVPGHDQRDPQRPPRGLAGH"
BASE COUNT          613 a          821 c          789 g          608 t
ORIGIN      
        1 tgacaccgga ggaggtcgcc cacacggttg ttatggaaat tcagggtttt gccaccggat
       61 atgaagtcat cacagccctg gattcgaccg tcatggatcc cagttatcgg gctggagtgc
      121 tgcgccaacc ccttatcgca gagatgcagc ggctcagcga cgaaaaacag acccccagtg
      181 tggcgcttgg gcagcttgga ccgccagaac tctccaagct gttgtatgaa gcctggcttc
      241 tgcgcaccaa gtttcgcagc atgaaaaagg tgctctctgc ggatcccgct ctgatcagcc
      301 atgaactgga gaattacctg ctgcatcatc ccattcgtca taccatcgtc tccatcggca
      361 tccccatcct gcttgcggac ggcacgacac ttctgcgcgg cccccaaatc aaggtacccg
      421 cgggccgtgt caaggatgaa ttgaatgtaa cccctgagac aatagatgca tgggctagaa
      481 agggttgggt ggatctgcgt ccggcaaaca tggccaaatg gcaagaacgg atcgaggcga
      541 tgttcgattc ctgcaagaac ttttttaatg tggatgccag cgatatcagc cgtggaccct
      601 gcccggcgga aacccttcgc atcggtgaga tggtggggtg ggtctttaac aatgatcccg
      661 ctatccaggg attccgggtc aagtccttct gaccagaatg agcggcaacg ggcatgggaa
      721 gcgcacctgc agagaatcct gaccctcgca gagttcagga tagccccgat tcatcaggcg
      781 agaatgagcg gatcctcatc accctccccg tcttcaacga agcgctgcac ctcgggcagc
      841 tcatcaccga tttgcggaag gtgacagacc tggatatact ggtcatcgat gacggttcgg
      901 aagatgatac gccagctgtg ctgcagagag tgggcgcgtc atgccttcgc cacaaccgca
      961 actacggcaa gggggaggcg ctgcgctccg ccctgaaggc cgcgcgggcg tggggttatc
     1021 actggatcat caccatggat ggggatggcc agcacgatcc cgccgctctc ccggtattta
     1081 tccgcactat acggtcgcac caggctgatt tttatatcgg ctggcggcag gggagaggcg
     1141 gcatcatgcc ccccctgcga cagatcagca acggcatcag ctcggttttg ctctcccttt
     1201 tcgccggtgt ccgttttcat gacgcacagt gcggcttccg cgcctatcgt accgccattc
     1261 ccggccttga cgcctgccgc gaacagggtt ttcagtatga gagcgaactc ttgctgcgcc
     1321 ttggccgcct cgatctgcgc gtcgacgaga tccccatcgc tactcgttat atcggcagtt
     1381 cgagccgtat ccgctatctt cgtgataccc tcgctttcct gcgcctgctc tggcgttcgc
     1441 tgtggtggta gaatgctagt gcatgccgtg gcggcaccgt ccgtgtattt agacaaatat
     1501 ttgcttgtaa tcatcgctta attgttgtat atttttatcc attttcgggg cgtagcgcag
     1561 tttggctagc gcgcctggtt cgggaccagg aggtcggtgg ttcaaatcca ctcgccccga
     1621 ccctaaagcc catccggttt catctggatg ggctttttag ttggcatgaa ttttccatcg
     1681 taattattga ttttataata aatattacct atatttcagg attaaaaaca tttgcaccca
     1741 ttggcaggaa tatgcacatg agctccgact atacctttac cgagatcgaa cacaagtggc
     1801 aggcccgctg gcgtgagagc ggcctttacc ggacggatct gaacgaaacc gcgaaaaaac
     1861 tctactgcct ggtgatgttt ctttacccct cgggcgaccg gctccacatt ggccactggt
     1921 acaactacgc cccgaccgat acctgggccc gtttcaagcg catgcagggt ttcaacgtct
     1981 tcgagcccat gggttatgac gctttcggcc tgccggccga gaattatgcc attaaaaagg
     2041 gcgttcatcc cgctgtcagc acagccgaaa atatcgccaa tatccgcaag cagctcgagg
     2101 ccatgggggc aatgtacgac tgggatcatg aggtcaacac cagccatccc gattactacc
     2161 gctggacgca gtggctcttc ctcaagatgt atgaacacgg ccttgccttc cgcaagcagc
     2221 aggcggccaa ctggtgtccc ggctgcaaga ccgttctcgc caatgaacag gtgatcgacg
     2281 gtcactgcga acgctgcgag agcgaagtgc tgagcaagga tctcaaacag tggtttttca
     2341 agaccaccgc ctacagcgaa cggcttctgg aggggcaccg gaccatcgac tggccggaga
     2401 aaaccctgct gatgcagaaa aactggatcg gcaagagcgt tggcgcgacc attgatttca
     2461 agctcgaggg gagggaggag aatatccagg tcttcacgac ccggccggac accctctggg
     2521 gcgttaccta catggtgctg gcgccggagc accccctcgt cgagcgcctg accacgccgg
     2581 aacacaaggg cgcggtcgcc gaatatgtcc tgcaggcgcg caaacagcgc gagatcgacc
     2641 gcctttcgac cgaaaaggag aagaacgggg ttttcatcgg cagttactgc accaatccgg
     2701 tcaatcagga acgggtgccg atctggatcg ccgattacgt actggtcact tacggcaccg
     2761 gtgcggtcat ggcagtaccc ggccatgatc agcgcgatcc gcaacgcccg ccacgaggtc
     2821 tggctggcca c
//
LOCUS       DAOPWU010000417         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_37571_length_2786_cov_4.008422, whole genome shotgun sequence.
ACCESSION   DAOPWU010000417 DAOPWU010000000
VERSION     DAOPWU010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37571_length_2786_cov_4.008422"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="PKM23_14520"
     CDS             complement(<1..1522)
                     /locus_tag="PKM23_14520"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNY92735.1"
                     /translation="MKHQYLLFAALALLTLAMTARAGEEILPPLTSPAVVQTNGPQGR
                     LSHGDWWSNSAGGNQPHLFYLYVPLAVPDNFTIRVELYDPECFQTGSELDELKGSAWD
                     ETRFRLLAPDGSTVVADVTWPAAASTSERWNSFAEFTPGQYGRGVFLIQATTAEDDEN
                     TYRLRIVESDPDNTPGSGDEMHLAIGRSALQMLARATATLHLYAPPGSEEMLLANFGM
                     DNNEAVVYLSPTGQILAGTVSGDGVWNNSTSAQLPPPGGDLITAPQPGWWSLQFTAAA
                     DNQFSLYGPQPFLMSEAPAAPRLSALLQDGRTQVRKGEHVTYSATIRNQGSAAAQTCT
                     LAVALSPGLVITQPGEGTITPTGGWLWTTGLLPAGAERTLNFTVFISSESTSPVAATA
                     LASGQDLLFQRLSSAPAPDIDQLVVAGSIAGLIWQDLNHDGKLDSGEPGISGIKIDLY
                     SPTLEILASDTSDGTGAYAFPSLPIGTYQVRPDAAFLPDGWDATTTVQGEWLTITDL"
     gene            complement(1672..>2786)
                     /locus_tag="PKM23_14525"
     CDS             complement(1672..>2786)
                     /locus_tag="PKM23_14525"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92736.1"
                     /translation="GMYFSLPRTGGDILTDSEVDTSSGRTPIFTLANQSNSIGWNAGY
                     MSSPLVVPEIKPAANLVLNVAVSNDSAHVGEYVDFTIDVTNRGPDASLSPHLVMQLAP
                     NLIFVSASPAPVDPDNNPLEWSLETLVMSEMRRVTVRTLVVDDGEATGIIYLLTTTEE
                     TDQTDNSYQYVLRDGLPVELTEFIAHFNGRGVLVKWVTASETENFGFQLFRAEEEQGR
                     YERINPAIIEGAGTSSSEHSYEYLDTEVIAGTTYYYKLMDVDYSGQMAWHGPVTAVAN
                     QPAAFALSQNYPNPFNSQTRIRFTIGQPAHAEMTIFNQLGQKVRSLFAQEWPAGQHEV
                     IWDGTDEYNLPVASGLYIYLFRSGDYTEKKIMNLIK"
BASE COUNT          479 a          782 c          991 g          534 t
ORIGIN      
        1 ccaggtcggt gatggtcagc cactcgccct gaacggtggt ggtggcatcc cagccgtcgg
       61 gcagaaaagc ggcatccggc cgcacctgat aggtgccgat gggaagagag gggaaggcgt
      121 aggcgccggt tccgtcgctg gtgtcggagg cgaggatctc gagggtcggg ctgtagagat
      181 cgatcttgat gccggagatg ccgggttcgc cgctgtcaag cttgccgtca tggttgaggt
      241 cctgccagat cagtccggcg atggagcccg caaccacaag ctggtcgatg tcgggagccg
      301 gcgcgctgga caaccgctgg aagaggaggt cctggccgga ggcgagggcg gtggccgcga
      361 ccgggctggt gctttcgctg ctgataaaga cggtgaagtt cagggtgcgc tcggcgccgg
      421 ccggcagcag accggtcgtc cagagccagc cgcctgtggg ggtgatggtg ccttcaccgg
      481 gctgggtgat gaccaggccg ggcgagaggg ccaccgccag tgtgcaggtt tgcgctgcag
      541 cgctgccctg gttgcggatc gtcgccgagt aggtgacatg ttcgcccttg cgcacctggg
      601 tgcgcccgtc ctggaggagg gcgctgaggc gcggagcagc cggggcttcg ctcataagga
      661 agggctgggg gccgtaaaga gagaactggt tgtccgcggc agcggtgaac tggagtgacc
      721 accatcccgg ctgcggtgcg gtgatcagat ctccgcccgg agggggcagc tgcgcgctgg
      781 tgctgttatt ccagaccccg tcacccgaga ccgtgccggc caggatctgc ccggtcgggg
      841 agagatagac caccgcttcg ttgttatcca tgccgaaatt ggccagcagc atctcttcgc
      901 tgccgggggg ggcatagagg tggagggtgg ccgtcgccct ggcgagcatc tgcagggcgc
      961 tgcgcccgat ggcgaggtgc atctcatcgc cgctgccggg ggtgttgtcc ggatcgctct
     1021 cgacgatgcg gagacggtag gtgttttcgt catcctcggc ggtggtagcc tggatcagga
     1081 aaacgccgcg gccgtactgg ccgggggtga attcggcaaa gctgttccag cgctccgagg
     1141 tcgaggcggc tgcaggccag gtgacgtcgg ccaccacggt cgaaccgtcc ggcgccagca
     1201 gccggaagcg ggtttcgtcc caggcgctgc ccttgagttc atcgagctcg ctgccggtct
     1261 gaaaacactc cggatcgtag agttccaccc ggatcgtaaa gttatccggc accgccaggg
     1321 gcacataaag atagaagaga tggggctggt tccctccggc gctgttgctc caccagtcgc
     1381 cgtgactcag acggccctgg gggccgttgg tttgcaccac ggccggagag gtgagcgggg
     1441 gcaggatctc ttccccggcc ctggcggtca tcgccagggt gaggagcgcg agggctgcga
     1501 acaacagata ctggtgtttc attttatacc tcccgagtcg cctgacatct gtccggcgcg
     1561 ccgctgcacc cgaggaggta agggcaaagg gtgtctgatg gtgcagcggt tgcagctgtg
     1621 caatgcacgt gcagcgggcc gcaagggagc accctcccgg cccctgcatc cctatttgat
     1681 caggttcatg atctttttct cggtatagtc gcccgagcgg aagaggtaga tgtagaggcc
     1741 gctggcgacg ggcagattgt attcatcggt gccatcccag atcacttcat gctgcccggc
     1801 gggccactcc tgtgcgaaga ggctccgtac cttctgtccg agctggttga agatggtcat
     1861 ctcggcatgg gcaggctgcc cgatcgtgaa gcggatgcga gtctggctgt tgaagggatt
     1921 gggataattc tgcgaaagcg caaaggccgc cggctggttc gcgaccgcgg tgacggggcc
     1981 gtgccaggcc atctgaccgc tgtaatcgac gtccatgagc ttgtagtagt aggtggtgcc
     2041 ggcgatgacc tcggtgtcga gatattcgta gctgtgttcg gaggaggagg tgcccgcgcc
     2101 ctcgatgatg gccggattta tcctttcata ccggccctgc tcctcttcgg cgcggaagag
     2161 ctggaagccg aaattttcag tctccgaggc ggtgacccat ttgaccagga cgccgcggcc
     2221 gttaaaatgc gcaataaatt cggtgagttc gaccggcagt ccatcccgga ggacgtattg
     2281 atagctattg tcggtctggt cggtctcttc ggtggtggtg agcagataga tgatgcctgt
     2341 ggcttccccg tcgtcaacga ccagggtgcg caccgtcacc cggcgcatct cgctcatcac
     2401 cagggtctcg aggctccact ccaggggatt attgtccgga tcgacgggag caggggaggc
     2461 gcttacgaag atgaggttgg gcgccagctg catgaccaga tgcggcgaga gcgaggcgtc
     2521 gggcccgcgg ttggtgacat cgatggtgaa atcgacgtac tccccgacat gggctgaatc
     2581 gttgctgacg gcgacattga ggacgagatt ggccgccggc ttgatctccg gcaccaccag
     2641 aggtgaactc atgtaacccg cgttccagcc gatggaattg gactgattgg ccagggtaaa
     2701 aatgggcgtc cggccgctgc tggtatcgac ttcggaatcg gtcaggatgt cgccgcccgt
     2761 ccggggcaga ctgaaataca tccccg
//
LOCUS       DAOPWU010000418         2782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_37635_length_2782_cov_4.972864, whole genome shotgun sequence.
ACCESSION   DAOPWU010000418 DAOPWU010000000
VERSION     DAOPWU010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37635_length_2782_cov_4.972864"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..446
                     /locus_tag="PKM23_14530"
     CDS             261..446
                     /locus_tag="PKM23_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92737.1"
                     /translation="MTIKEMAIKTIQNLPDDANWDDIQERIAFIAGVRKGLRELDEGK
                     GIGHDRIKEEYAEWLAN"
     gene            797..931
                     /locus_tag="PKM23_14535"
     CDS             797..931
                     /locus_tag="PKM23_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92738.1"
                     /translation="MDLIVGNRIGQLFGYQLPAGFHLLTAFPNQQQIVTMRQKMKCTG
                     "
     gene            1000..1857
                     /locus_tag="PKM23_14540"
     CDS             1000..1857
                     /locus_tag="PKM23_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92739.1"
                     /translation="MQIVKIADANRFVTADSLTISLCNVRCFSIADADSFKRAFAVMV
                     VRDAEKILGDRELLAEVASRADSLILVHLWEERKVFGKRSLNELWLANGWGWYEEEPS
                     GRYAKFYRRAAGQAEKGNRGIHDQTLFRKPRPAIAGMWLSLGLGRGEYKDKSPDWDDH
                     LMLDLGLHLRKKKLVLSGGIQGIYLSEASSAGASYLLAGRSFYGEGAEMVVSMGGSIN
                     QWSYNTESSRGIIRSSSYPGLQAKVQMIGHFPQALGFALDLDANLNKEKSYYAFSLHL
                     ILGGWKYAR"
     gene            1901..>2782
                     /locus_tag="PKM23_14545"
     CDS             1901..>2782
                     /locus_tag="PKM23_14545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY92740.1"
                     /translation="MKQLKHNIPLFLWQRFLNDFLLIAPILIPFYQYNRLGAFAFYLS
                     QATYALTVFVMEVPSGYLADVIGRRKTLIAGALLYPLGLTIYALGSSLPMFLAGEFVL
                     AVGHSMRSGSDSAMLYDTLKKLTREGEYAGIEGRGNQFARIGTAIASVSGGLLATVSM
                     RLPFWFNILIGLAIIPLAATMTEPERKKASGQNPLQNILHIAAESMKNPDIRPFILLL
                     GLVGSVSVISLWAYFLYYQAVGIPLALFGVLFAAFQFSGALGARFSAAAAAKAGTLHL
                     LMVSLLIAPILLLVGLLR"
BASE COUNT          603 a          761 c          740 g          678 t
ORIGIN      
        1 cagcacaacc ggggtgggaa cgagattttt gctggccggt ggtgagtgcc cgctgacggg
       61 cagtatcgta tcatcattga cttcagtatg ctgaattcgt acattacata atagtaggcc
      121 tggcatagtc ataaatcttt gcgatttact gtcgcgtctc gatggaatca ctcaaatgac
      181 aagcttttgc catggagaag tattcctcca tctgttaatg gatctttctt agctgcttgc
      241 tcgattttga ggtgtgaagt atgacaataa aagaaatggc cattaaaacc atccaaaatt
      301 tgccggacga tgcaaattgg gatgacattc aggaacgcat agcctttata gctggagtgc
      361 gtaaaggttt acgtgagctc gacgagggaa aaggcatcgg tcatgatcgg ataaaagagg
      421 aatatgcgga gtggcttgca aactgatctg gtctccctca gcccggatcg atctgcaaga
      481 attgatttac ttcatcgccg agtagaatcc aggctctgct cgacgatttg gcgataaact
      541 gtttcagact gtggaaagat tgagtatctt tccggaatca ggacggatcg tacctgagtt
      601 tggcgatcca ctcattcggg agattatccg caaaccttgc agaattgtct acagactcga
      661 tcctgaaaaa ctaatcgtag aaatagtcag gatctggcac agcgccaggg gaatacctca
      721 gatctgaagg tctcaccaat tcccccttat ttagggtgcg gtccctgtta tcggcgatat
      781 cgaccaggac ggcaatttgg atttgatcgt gggcaaccgc atcggtcagc tcttcggcta
      841 tcagctgccg gcgggatttc acctgctgac ggcttttccc aatcagcagc agattgtcac
      901 catgcgccag aaaatgaaat gtaccggatg aataatgtac tttactccct ttttctcact
      961 gctttttgcc tctgtccgtt cactttcgcg gccgataaag tgcaaattgt taaaattgcg
     1021 gacgccaacc gatttgtcac tgctgacagc ctgaccattt ccctctgcaa tgtcagatgc
     1081 ttttccatcg ccgatgccga ctccttcaaa cgggcattcg ccgtgatggt ggttcgagat
     1141 gcagaaaaaa tactggggga ccgggagctg ctcgccgagg tggcgagccg ggccgattca
     1201 ctgatcctgg ttcacttgtg ggaagagaga aaggttttcg gcaaaaggtc gcttaacgag
     1261 ctctggctgg ccaacggctg gggctggtat gaagaggagc catctggcag gtatgccaaa
     1321 ttctaccgga gggccgccgg tcaggctgaa aagggaaatc gcgggatcca tgaccagacc
     1381 cttttccgga aaccccgacc ggccattgcc ggcatgtggc tcagtctcgg cctcggaagg
     1441 ggagagtata aagacaagag ccctgactgg gatgaccacc tcatgctcga tctcgggctt
     1501 catctcagga agaagaagct ggtcctctcc ggcggaatcc aggggattta cctgtctgag
     1561 gcctcctcag caggggcgag ctatcttctg gccgggagat cattttacgg agagggcgct
     1621 gaaatggtgg tctccatggg cggcagcatc aatcagtgga gctataatac ggagagcagc
     1681 cggggcatca tccggtccag ctcctatccg ggcttgcagg ccaaggtgca gatgattgga
     1741 catttcccgc aggcgctggg cttcgcgctg gatctcgatg ccaacctcaa caaggagaaa
     1801 agctattatg ccttttctct ccatctgatc ctgggcggat ggaagtacgc ccgttaggta
     1861 atcctgttca cctgtacgct gcagggatga gaaccgagac atgaagcagc tcaaacacaa
     1921 tatccccctc tttctgtggc agcggttttt aaatgatttc ctcctgatcg cgccgatcct
     1981 gattcccttt taccagtaca accggctcgg cgccttcgcc ttttatctct cccaggccac
     2041 atatgccctg accgtttttg tcatggaggt tccctccggc tacctggccg acgtcatcgg
     2101 ccggcgcaaa acgctgatcg ccggcgccct cctctatccc cttggcttga cgatctatgc
     2161 cctcggttcg tctctgccca tgttcctggc cggagagttc gtgctggccg tcggccacag
     2221 catgcgctcc ggaagtgatt cggccatgct ctacgacacc ctgaaaaaat tgaccaggga
     2281 aggggaatac gccggcatag aagggagggg caaccagttc gcccgtatcg gcaccgccat
     2341 cgcctccgtc agcggcggtc tgctggccac cgtctccatg cgcctgcctt tctggttcaa
     2401 catcctcatc ggactggcga tcattcctct ggccgcaacc atgaccgagc cggagcggaa
     2461 aaaagccagc gggcaaaatc ccctgcaaaa tatcctgcac attgcggccg agtcgatgaa
     2521 aaatcccgat atccggccgt tcatccttct ccttgggctg gtcggatcgg tcagtgtcat
     2581 ctccctgtgg gcttattttc tctattatca ggccgtcggc attcccctgg cccttttcgg
     2641 cgttctcttc gccgcctttc agttttcagg cgccctcggc gcccggtttt cagctgcagc
     2701 ggcggccaaa gccggaacac tgcaccttct catggtgtcc ctgctcatcg cgcccattct
     2761 tctgctggtt ggactgctgc gc
//
LOCUS       DAOPWU010000419         2749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_38315_length_2749_cov_3.506682, whole genome shotgun sequence.
ACCESSION   DAOPWU010000419 DAOPWU010000000
VERSION     DAOPWU010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38315_length_2749_cov_3.506682"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /locus_tag="PKM23_14550"
     CDS             <1..1934
                     /locus_tag="PKM23_14550"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HNY92741.1"
                     /translation="ESDRDRLVRMGREGIPVAERLFGRGYRLRPNFIQPYNCRNVLIE
                     GVRIKGSPMWEIHPVLCTNVTVSGVHIDSHGPNNDGCNPESCRDVLIENCYFDTGDDC
                     IAIKSGRNEDGRRLNVPSENIIVRGCTMKDGLGAVVIGSEISGSCRNVFTEDCVMDSP
                     NLDRALRIKTNSFRGGVVENIMMRNVRIGEVKQAVLLVDFMYQEGDGGLFTPVVRNLR
                     LENITSRKSRHALHLVGYARSPISDITITDCRFDGVERGSVLEHVTGLRMERVSINGE
                     PADTPATWAEKMAGSIMKRSPVIYDEWDYVTGTVLKGFEELWRETGDARYFAYIKSTV
                     DSVVSPFGAIADYKPLEFNIDEVREGTVLLFLARETGDLRYKVAADQLRQQLRDQPRT
                     KAGGFWHKKRYPWQMWLDGLYMGSPFYAEYAARYGEPAALADVVRQFTLLEEKARDTR
                     TGLFYHGWDESREQSWADPQSGCSANFWGRGLGWYAMALVDVLDYLPAGHSGRDSLVQ
                     ILNRLAPAIAGAQDPATGLWWQVLDQGGREGNYLESSASAMFVYTLAKGVRLGCLDPA
                     WMGAAEKGFKGMIDHFIKHNPDGTISLTDVCVTAGLGYGRDGSYDYYVHQTEARDDDG
                     KGLGPFILAGVEMELRRGR"
     gene            1955..>2749
                     /locus_tag="PKM23_14555"
     CDS             1955..>2749
                     /locus_tag="PKM23_14555"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HNY92742.1"
                     /translation="MRKICCSLLLAALLLAACTSEVEKKLRQEYPGKVLLTIKNPLAN
                     DRPDEVVAIPLDLIRAKAADFNPRAVVLAAKGRELPVQLCDDNGDGAPDHLLANLDAG
                     AGERSRLILRYAPEGVKERIYTPRAHAELSAKFGGEWVQRKYVSGTFHKVDFLRVPPE
                     HTDHSEYIRYEGPGWESDRVGFRFYLDWRNATDIFGKRSRELVLHRVGLEDFENYHHM
                     SDWGMDILKVGPALGIGTVAWWDGASANRVAVTDSIHCRVAAAGPLL"
BASE COUNT          523 a          897 c          843 g          486 t
ORIGIN      
        1 cggaatccga ccgcgaccgc ctcgtgcgca tgggacgcga ggggattccc gtcgctgaac
       61 gcctctttgg aagaggctat cgcctccggc ccaatttcat ccagccctat aactgccgca
      121 atgttctcat cgagggcgtg cgcatcaagg gctcgccgat gtgggagatc catccggtgc
      181 tctgcaccaa cgtcacggtg agcggcgtcc atatcgacag ccacggcccc aacaacgacg
      241 gctgcaatcc ggaaagctgc cgggacgtcc tgatcgagaa ctgctatttc gacaccggcg
      301 atgactgcat cgccatcaaa tccggacgca acgaggacgg ccgccgcctc aacgtgccga
      361 gcgaaaatat catcgtgcgc ggctgcacca tgaaggacgg cctcggcgct gtggtcatcg
      421 gcagcgagat ctcgggcagc tgccgcaacg tctttacgga agattgcgtg atggacagcc
      481 ccaatctcga tcgcgccctg cgcatcaaga ccaactcttt ccgcggcgga gttgtcgaga
      541 atatcatgat gcgcaatgtg cgcatcggcg aggtgaaaca ggcggtgctg ctggtcgatt
      601 ttatgtacca ggagggcgac ggcgggctct tcaccccggt ggttcgcaac cttcgcctcg
      661 agaatatcac cagccggaaa agccggcacg ccctgcatct ggtcggctat gcgcgctcgc
      721 cgatcagcga tatcacgatc acggattgcc gtttcgacgg cgtggagcgg ggcagcgtcc
      781 tggagcacgt caccgggctc aggatggagc gcgtttcgat caacggcgag cctgcggata
      841 ccccggcgac ctgggccgaa aaaatggccg gctcgatcat gaagcgcagc cctgtcatct
      901 atgatgaatg ggattatgtg accggcacgg tgctcaaggg ttttgaagag ctctggcgcg
      961 aaaccggcga tgcccgctat tttgcctaca tcaaatccac cgtcgactcg gtggtgagcc
     1021 ccttcggcgc catcgccgat tacaagccgc tggaattcaa catcgacgag gtgcgcgagg
     1081 gcacggtgct cctctttctc gcccgggaga ccggcgatct gcgctacaag gtggcggccg
     1141 atcagctgcg tcagcagctc cgcgaccagc cgcgcaccaa agcgggcgga ttctggcata
     1201 aaaagcgcta cccctggcag atgtggctcg acggccttta catgggcagc cccttttatg
     1261 ccgaatatgc agcccgctac ggcgagcccg cggctctggc tgacgttgtc cgtcagttca
     1321 ccctgctcga ggagaaagcg cgcgataccc gcaccggcct gttctaccac ggctgggacg
     1381 agagccgcga acagagctgg gccgatccgc agagcggctg cagtgccaac ttttggggac
     1441 gcggcctcgg ctggtacgcc atggcgctcg tcgacgtcct tgactacctg cccgccggcc
     1501 acagcggccg cgattccctg gtgcagatcc tcaaccgcct tgctcccgcg atcgccggtg
     1561 cccaggatcc ggccaccggc ctctggtggc aggtgctcga ccagggcgga cgcgaaggca
     1621 actatctcga gtcctcggcc tcggccatgt tcgtctatac cctggccaag ggcgtccggc
     1681 tcggctgtct cgatcccgcc tggatggggg ccgctgaaaa aggattcaag gggatgatcg
     1741 atcactttat caaacacaac cccgacggca ccatctcact gacggatgtc tgcgtcaccg
     1801 ctgggctcgg ctacggacgc gacggttcct acgactatta cgtgcaccag accgaggcgc
     1861 gggacgacga cggcaagggg ctcggcccct tcattctggc cggcgtggag atggagctgc
     1921 gccggggccg ctaacgcacg acaaggagcg taaaatgcgc aagatctgct gttctctgct
     1981 cctggcggcc ctgcttctgg ccgcctgcac ctcagaggtg gaaaaaaagc tgcggcagga
     2041 gtatccgggg aaggtgctcc tcaccatcaa aaaccccctc gcgaacgacc gtcccgatga
     2101 agtcgtggcg attccgctcg acctgatcag ggccaaagcg gccgatttca atccccgcgc
     2161 cgtggtcctg gccgccaagg gccgcgaact gcccgtccag ctttgcgatg acaacggcga
     2221 cggcgcgccc gatcacctcc tcgccaacct cgatgccgga gccggggaac ggagccggct
     2281 catcctccgc tatgcccccg aaggtgtaaa ggaacgcatt tacaccccgc gcgcccacgc
     2341 cgagctttcg gccaaattcg gcggcgaatg ggtgcagcgc aagtacgtca gcggcacctt
     2401 ccacaaggtt gactttttgc gcgtaccccc ggaacacacc gaccactcgg agtacatccg
     2461 ctatgaaggc ccgggctggg aatcggaccg ggtgggcttt cgcttttacc tcgactggcg
     2521 caacgccacc gatatctttg gcaaaaggag ccgcgagctg gttttgcacc gggtggggct
     2581 ggaggatttt gagaactatc accacatgtc cgattggggc atggatattc tcaaagtcgg
     2641 tcccgccctc ggcatcggca cggtcgcctg gtgggacggt gcaagcgcca accgcgtcgc
     2701 cgtcaccgac agcatccact gccgcgtcgc cgcggccggg cccctgctc
//
LOCUS       DAOPWU010000420         2740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_38509_length_2740_cov_4.153445, whole genome shotgun sequence.
ACCESSION   DAOPWU010000420 DAOPWU010000000
VERSION     DAOPWU010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38509_length_2740_cov_4.153445"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..1720
                     /locus_tag="PKM23_14560"
     CDS             458..1720
                     /locus_tag="PKM23_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HNY92743.1"
                     /translation="MQISGLKYGAQLLELVEFPYAPFLTADATKEEIQNLLNAHKKLV
                     VKPFFSGGVGKKGKAGLVRIVDNVHDALLAKRDLYFATHHYGSKTVVANGVTYEAFIP
                     SEIEVYFSITASTLHRKPIFTISPWGGVDIEALPESKKKVTWIDPFIGLKSFDVTNAL
                     MDTGCPEAFISPLVVNLPKLWGLFDGYGLTTLELNPIRMARIDNRIVPVACDLKAAFD
                     QDNPAWKRLGLPSTIFQSDITPFEAEINQLRTYQGQSDVLELNPTGTIIPFMFGGGAN
                     SAATETLGSSAIFASDFGGNPPYEKMYDIARIVFKNWLSRANVLLIIGGKANNTDIYV
                     TFKGIFDALRDYVAAHGPRPVYVVVGRGGPNLIKGMFYAKDILDTLKLPYKMFGYDTS
                     MIQVLEYAKQIDQWWADKGKKEYEKKKV"
     gene            1724..>2740
                     /locus_tag="PKM23_14565"
     CDS             1724..>2740
                     /locus_tag="PKM23_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNY92744.1"
                     /translation="MRQQKTKPFPYFIGIHSLEELVTKDSRVCVINILGNESRKVTPV
                     SHEYSGGNIVAGVQYGRKGSLETRAGNIPVYSSIRDVIQSGIFFDIGVIYLPPAAVGQ
                     SVAELVTYNEALKRIVIVTEKVPTRDSRNIRFVCQEAGVDVIGANCLGVANVWDHVRV
                     GGALAGDHPEEALRQGSVAIHSNSGNFTTTIAEYLRTAGFGLSTAVSSGKDVYIHFAL
                     PEFLYAAQNDPRTKAIALYVEPGGYYERQALDWIKERRFGFNKPIVVCVTGRWKKDIQ
                     RSCGHAGALAGSGDDAISKEQWFDDYFGVPAYNPDHPRVSKRGVRVSSIQHFPEAMKA
                     VFEAI"
BASE COUNT          599 a          876 c          716 g          549 t
ORIGIN      
        1 gcaccgagcg catcggcgac aaccgcatca tcaacccctg actcccccct tttttacctt
       61 caaatctggc tcccacccga tttaattaaa tgataataat gggcttaagg caacataagg
      121 cctgtaataa taaatatggc cgcctatctg gccagtgcac aggccgcaag tttgctggaa
      181 atagcaggga gggattgagg ggcttggcgc caggtctcag gtggggacaa gatttcgagc
      241 ggggtacgcg gcaccaggcg tcaggtggga gccagatttc gggggtaccc tgcccttttt
      301 taccttgctt ctcagccggg gattatgtaa actatccgct gaaaaccaac tctcacagag
      361 gccaaagaga gagaagaata gaggccttga catgccctgc tgtgatcttt ccctctgtgt
      421 gaatccaatc ttatcccatt agaatcaggc agaagacatg cagatttccg gactcaagta
      481 tggcgcgcag ctgctggagc tggtggaatt tccctacgcc cccttcctca ctgccgatgc
      541 caccaaggag gaaatccaga acctcctcaa tgcccataaa aaactggtcg tcaaaccctt
      601 cttcagcggc ggcgtcggca aaaagggcaa ggctggtctg gtccgcatcg tcgacaatgt
      661 tcacgatgcc ctcctggcca agcgcgacct ctattttgcc acccaccatt atggctccaa
      721 gaccgttgtg gccaatggtg tcacctatga ggcctttatc ccctctgaaa tcgaggtcta
      781 tttcagcatt accgcctcga ccctgcaccg caaaccgatc ttcaccatca gtccctgggg
      841 cggcgtggat atcgaagccc tgcccgagag caaaaagaaa gtcacctgga tcgatccctt
      901 catcggtctc aaatccttcg atgtcaccaa tgccctcatg gacaccggct gcccggaggc
      961 cttcatctcg cccctggtgg tcaatctgcc caagctctgg ggactgttcg acggctatgg
     1021 cctgaccacc ctggagctca acccgatccg catggcccgg atcgacaacc gcatcgtgcc
     1081 ggtggcctgc gatctcaagg ccgccttcga ccaggacaat ccggcctgga aacgcctcgg
     1141 gctgcctagc accatcttcc agtccgatat cacccccttc gaggcggaga tcaaccagct
     1201 ccgcacctat caggggcaga gcgacgtgct cgagctcaac cccaccggca cgatcatccc
     1261 tttcatgttc ggcggtggag ccaactcggc ggctacggag accctcggca gctctgccat
     1321 tttcgcctct gatttcggcg gtaatccccc ctatgaaaaa atgtacgata tcgcccgcat
     1381 cgtctttaaa aactggctca gccgcgccaa cgtgctgctc atcatcggcg gcaaggccaa
     1441 caacaccgac atctatgtga ccttcaaagg tatttttgat gccctgcgcg actacgtcgc
     1501 cgcccacggt ccccggccgg tctatgtcgt ggtcggccgc ggcggcccca atctgatcaa
     1561 ggggatgttt tacgccaagg atatcctcga caccctcaaa ctcccctaca agatgttcgg
     1621 ctacgacacc tcgatgattc aggtgctgga gtacgccaaa cagatcgacc aatggtgggc
     1681 ggataagggc aaaaaagaat acgaaaagaa gaaggtgtga gcgatgagac agcagaagac
     1741 gaaaccattt ccctatttta tcggcattca ctctcttgag gagctggtca ccaaggattc
     1801 acgggtctgc gtgatcaata ttctcggcaa cgagagccgc aaggtcactc cggtctcgca
     1861 tgaatacagc ggcggcaata ttgtcgccgg cgtccagtac ggccgcaagg gttccctcga
     1921 gaccagggcc ggcaatatcc cggtctacag cagtatccgc gatgtgatcc agagcggcat
     1981 cttttttgat atcggcgtca tctatctgcc ccccgccgcg gtcggccagt cagtggcgga
     2041 gctggtcacc tataacgagg ctctcaaacg catcgtcatc gtcaccgaaa aggtgccgac
     2101 acgcgattcg cgcaacatcc gcttcgtctg ccaggaagca ggcgtcgacg tcatcggggc
     2161 caactgcctc ggcgtcgcca acgtctggga ccatgtccgc gtcggcggcg ccctcgccgg
     2221 cgaccatccc gaggaggccc tgcgccaggg ctcggtggcc atccactcca attcgggcaa
     2281 ctttaccacc accatcgccg aatatctgcg caccgccggc tttggactca gcaccgccgt
     2341 cagctcgggc aaggacgtct acatccactt cgccctgcct gaattcctct acgccgccca
     2401 aaacgacccg cgcaccaagg ccatcgccct ttatgtcgag ccgggcggct attacgaaag
     2461 acaggccctg gactggatca aggagcgccg cttcggcttt aacaaaccca tcgtcgtctg
     2521 cgtcaccggc cgctggaaaa aggatatcca gcgctcttgc ggccatgccg gggccctcgc
     2581 cggctcgggg gatgacgcca tctccaagga gcagtggttc gatgactatt tcggtgtacc
     2641 cgcctacaac cccgaccatc ccagggtgag caaacgcggg gtgcgggtct cctcgatcca
     2701 gcacttcccc gaggcgatga aggccgtctt cgaggcgatc
//
LOCUS       DAOPWU010000421         2714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_39017_length_2714_cov_3.652125, whole genome shotgun sequence.
ACCESSION   DAOPWU010000421 DAOPWU010000000
VERSION     DAOPWU010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39017_length_2714_cov_3.652125"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /gene="rplU"
                     /locus_tag="PKM23_14570"
     CDS             <1..261
                     /gene="rplU"
                     /locus_tag="PKM23_14570"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNY92745.1"
                     /translation="QINAPRLAGEAGSTVEFEKIILLSGDSGDVKVGKPYVEGAKVQA
                     TIVGHGQDKKVTIFKKIKSEGYTLFKGHRQQHTTLKIDAIIG"
     gene            302..553
                     /gene="rpmA"
                     /locus_tag="PKM23_14575"
     CDS             302..553
                     /gene="rpmA"
                     /locus_tag="PKM23_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNY92746.1"
                     /translation="MAHKKGVGSSRNGRDSNPQMLGVKRFDGQVVPAGSILVRQRGTR
                     IHPGLNVGIGSDDTLFALKTGKVKFTTHGKSKKMVNIIL"
     gene            656..>2714
                     /locus_tag="PKM23_14580"
     CDS             656..>2714
                     /locus_tag="PKM23_14580"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNY92747.1"
                     /translation="MKMVLRWGIIPAFFFSLLACHVPALAQTPRLCINEYLASNSKLN
                     ADPDFNEYGDWIELYNPGDVAVPLVGWYLTDNLSRPDKWRITANVSIPARGWLVIWAD
                     DRNTGLHANYKLSADGEEIGLYDYALKPVDTISFGPQTTNVSCGRYPDGAGEWRYFST
                     PTPGAANWNQPFEGVVSDPLFSMPGGFYSAPQTIVLSCRDGDASIHYTLDGSVPTEGS
                     AAYLQPIVIDRTTVLRARAFRAGSLPSRVITNTCFIGESATLPVVSLSTNPENLWDDR
                     IGIYVVGTNGVTGYCMSTPCNWNQDWERPATLEYYDEAGQQQFRLDAGIKIGGGCTRK
                     YPQKPLAVYARSIYGSSKIYYPLFDDRGAEVYNNLVLSNGGQDWYRTFFRDGMMQNLI
                     KGRMDIDWVAYRPALLFINGKYWGIHSLREKHNEHYLEATHGIDPDRVDILSDNASVH
                     EGSNTLYKQLIEYVGSRDLAVREHFDYVSSQIDVDEYINYLIAEIYFANIDWPAGNIK
                     YWRAWGEGNRWRWILFDTDLGFGAHSDGQYDDNFLAAVTSPVATSYANPPWSTLLIRK
                     MLENEGFRDRFIQRFAGHIQTTFAPERVVHKIDSLQALIAPEIPRHKQRWPDAFNHGA
                     TWEGQVETMRTFARKRPEHMMAHIAARFALSGTAALSVRSNEPAWGRVEIEGVAMPDS
                     LTLL"
BASE COUNT          547 a          920 c          745 g          502 t
ORIGIN      
        1 cagatcaacg cccccaggct ggccggtgag gccggttcta ccgtcgaatt cgaaaagatc
       61 atcctgctga gcggcgacag cggtgacgtc aaggtcggca agccctacgt cgagggcgcc
      121 aaggttcagg cgaccatcgt cggccacggc caggataaaa aggtcaccat ctttaagaaa
      181 atcaaaagcg aaggctacac cctcttcaag ggccaccgcc agcagcacac cactttgaaa
      241 attgatgcaa ttatcggtta gatagcgggc cgccgcccgc tcttttcagg aggattttca
      301 tatggcgcat aaaaaaggcg ttggctcttc gcgcaacggc cgcgacagca acccgcagat
      361 gctcggcgtc aagaggtttg acggccaggt ggtcccggct ggaagcattc tcgtgcgcca
      421 gcgcggcacc cgcattcatc ccggcctcaa tgtgggcatc ggttcggacg ataccctttt
      481 cgctctcaaa acgggcaagg tcaaattcac cacccacggc aaaagcaaaa agatggtcaa
      541 tatcatcctt tagctccggc cgggtttgca gcattttgag cgcgatgaaa gtcgcgcttt
      601 ttttttatat ctaacgaagc gggggggagg cttccaggat aataggaggt gaatgatgaa
      661 aatggttctg cgatggggga ttattccggc gtttttcttc tccttactgg cctgccacgt
      721 tcccgccctg gcgcagacgc ccaggctctg catcaacgag tatctcgcgt ccaactcaaa
      781 actcaacgcc gaccccgatt tcaatgagta tggcgactgg atcgagctct acaacccagg
      841 ggacgtcgcc gtcccgctcg tcggctggta tctgaccgat aacctctccc ggcccgacaa
      901 gtggcggatc accgccaacg tctccatccc tgccaggggc tggctggtca tctgggccga
      961 tgaccgcaac accggcctgc acgccaatta caagctctcc gcggacggcg aggagatcgg
     1021 actttacgat tatgccctca agccggtcga taccatcagc ttcggccccc agaccaccaa
     1081 cgtctcctgc ggccgctatc ccgacggcgc cggcgagtgg cgctatttca gcacccccac
     1141 cccgggtgcg gccaactgga accagccctt cgagggtgtc gtctccgacc cccttttctc
     1201 catgcccggc ggtttttaca gcgcccccca gaccatcgtc ctctcctgca gggatggcga
     1261 cgcgtccatc cattacaccc tcgacggctc cgtgcccacc gaaggctccg ccgcctatct
     1321 ccagcccatc gtcatcgacc gcaccaccgt cctgcgcgcc cgggccttcc gcgccggcag
     1381 cctgcccagt cgggtgatca ccaatacctg ttttatcggc gaatccgcca ccctgccggt
     1441 ggtctccctc tcgaccaatc cggagaacct gtgggatgac cggattggca tctatgtggt
     1501 cggcaccaac ggcgtcaccg gctactgcat gagcaccccc tgcaactgga accaggactg
     1561 ggagcggccc gccaccctgg agtattacga tgaggcgggc cagcagcagt tccgcctcga
     1621 tgccggcatc aagatcggcg gcggctgcac ccgcaagtac ccccagaagc ccctggcggt
     1681 ctatgctcgc agcatctacg gcagcagcaa gatctattat cccctcttcg acgaccgcgg
     1741 cgccgaggtc tacaacaatc tcgtcctctc caacggcggc caggactggt accgcacctt
     1801 tttccgcgac ggcatgatgc agaacctcat caagggccgt atggatatcg actgggtcgc
     1861 ctaccggccg gccctgctct tcatcaacgg caaatactgg ggcatccact ccctgcgcga
     1921 aaagcacaac gagcactatc tggaggcgac gcacggcatc gaccccgacc gggtcgacat
     1981 cctgtccgac aacgcctccg tgcacgaggg atcgaacacc ctctacaagc agctgatcga
     2041 gtacgtcggt tcccgggacc tggcggtcag ggagcatttt gactatgtca gcagccagat
     2101 cgacgtcgac gagtatatca actacctcat cgccgagatc tatttcgcca acatcgactg
     2161 gccggccggc aacatcaagt actggcgtgc ctggggcgag ggcaaccgct ggcgctggat
     2221 cctcttcgac accgacctgg gtttcggggc ccactcggac gggcagtatg acgacaattt
     2281 cctcgctgcg gtcacttcgc cggttgctac cagctatgcc aatcccccct ggtcaaccct
     2341 gctgatccgc aagatgctcg agaacgaggg cttccgcgac cgcttcatac agcgcttcgc
     2401 cggccacatt cagaccacct ttgcgccgga gagggtggtc cacaaaatcg acagcctcca
     2461 ggccctgatt gcgcccgaga ttccgcgcca caagcagaga tggccggatg ccttcaacca
     2521 cggcgccacc tgggaaggcc aggtcgagac catgcgtact tttgcgcgca agcgtccgga
     2581 gcacatgatg gcccacatcg ccgccaggtt cgccctcagc ggcaccgccg cgctctcggt
     2641 gcgctccaac gagcccgcct gggggcgggt ggagatcgag ggggtggcca tgcccgacag
     2701 cctcaccctg ctct
//
LOCUS       DAOPWU010000422         2710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_39117_length_2710_cov_3.812806, whole genome shotgun sequence.
ACCESSION   DAOPWU010000422 DAOPWU010000000
VERSION     DAOPWU010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39117_length_2710_cov_3.812806"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PKM23_14585"
     CDS             <1..1031
                     /locus_tag="PKM23_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNY92748.1"
                     /translation="MLKEEVDEEDIAEVVSKWTGIPVVRMLESEKQKLLRMEERLHER
                     VIGQDEAVEAVSDAIRRSRAGLQDENRPIGSFIFLGSTGVGKTELARALAEFLFDDER
                     AMVRVDMSEYMERHAVSRLIGAPPGYVGYDEGGQLTEAVRRRPYAVVLLDEIEKAHPE
                     VFNVLLQVLDEGRLTDNQGRTVNFKNTIVIMTSNLGAQQIMERSQEINEKNRKKIHEE
                     IREEVLGLLKSTLRPEFLNRIDDIIVFHALRREEIKLIARLQLKHLQRLLERQDLSLE
                     VTPEVEEYLAEHGYDPAFGARPLKRLIQREMADALAIRLLEGDFPAGSMVQVIVKDKK
                     LDFKRKSV"
     gene            1110..1622
                     /locus_tag="PKM23_14590"
     CDS             1110..1622
                     /locus_tag="PKM23_14590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY92749.1"
                     /translation="MKQWFGLLTVLLVAVLMTGCGEKLTEDQLRANASDFESREQWKE
                     AADMYARLVKAYPKSKRADETLYRLGVLYANNLKDYQKSVDAYRQLISKYPKSNYVIQ
                     SSFMIGYRYANDIKDFDNAKKYYQEFLKKWPAHELASSVKWELDHLGKDISELELQLG
                     NPAPDAAEQK"
     gene            1739..2683
                     /locus_tag="PKM23_14595"
     CDS             1739..2683
                     /locus_tag="PKM23_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92750.1"
                     /translation="MRIIRIAAAIAIFFSSLGISSLQAQGEDEGIRVSASAQPNEVPL
                     NRTAVVTIRLEWAGDLDRYEITRFDNPLVENLNIIETASANQVQVVGGLQVARQEYQY
                     TVKPDGLGMAYVDGIIIRYADRETGKEYRLASSRLELKVVDPLPEPGSYLWLVILLSI
                     LLSSLIAIYLVRRIRARRAEAERLARSQAASEVSLEQKYGERLREEIDLRQPDLDVNA
                     AFGRLAGVLRRYLAAKYSLPLISATTSEVRGLMAEAGADERVIADTLAVLESADIAKF
                     SGAAQMRSELERAYTILEGMLGTDTAPRGGAESAKRDD"
BASE COUNT          585 a          757 c          815 g          553 t
ORIGIN      
        1 ggatgctcaa ggaggaggtc gatgaggagg acatcgccga ggtggtctcc aaatggaccg
       61 gcatcccggt cgtgcgcatg ctcgagagtg aaaagcaaaa gctgctgcgc atggaggagc
      121 gcctgcatga gcgggtcatc ggccaggatg aagcggtgga ggcggtctcc gatgccatcc
      181 gccgcagccg ggcgggactg caggacgaga accggcccat cggctccttc atctttctcg
      241 gctcgaccgg cgtcggcaag accgagctgg cgcgggcgct ggccgagttt ctcttcgacg
      301 atgagcgcgc catggtgcgc gtcgacatgt ccgagtatat ggaacgccac gcggtttcgc
      361 ggctgatcgg tgcgcctccg ggctatgtcg gttatgacga aggggggcag ctcaccgagg
      421 cggtgcggcg gcggccgtat gcggtggtgc tcctcgacga gatcgaaaag gcccatcccg
      481 aggtctttaa cgtcctgctc caggtgctgg atgaggggcg tctcaccgac aaccagggcc
      541 ggacggtcaa tttcaagaat accatcgtca tcatgacctc caatctcgga gcgcagcaga
      601 tcatggagcg ctcgcaggag atcaatgaga agaaccgcaa aaagatccac gaggagatcc
      661 gcgaggaggt gctgggtctg ctcaagagca cgctgcgccc ggaatttctg aaccgcattg
      721 acgacatcat cgtcttccat gccctgcggc gcgaggagat caagctgatc gcccggctgc
      781 agctgaagca cctgcagcgg ctgctggagc gtcaggatct ctctctggag gtgacgccgg
      841 aagtggagga gtatctggcg gagcacggct atgatccggc gttcggcgca cggcccctga
      901 aacggctcat ccagcgcgag atggccgatg ccctggccat ccgcctgctc gagggcgatt
      961 ttccggcagg aagcatggtg caggtgatcg taaaggataa aaaacttgat tttaagcgca
     1021 aaagtgttta agttggaaaa ttgtggaact tatatttacg aggcacgttt accacacatt
     1081 cacgaatgaa ttccagcgga ggagatgcga tgaaacagtg gtttggattg ttgaccgttt
     1141 tacttgtcgc tgttttgatg accggctgcg gcgaaaaact gacggaagac cagctgcgcg
     1201 ccaatgcctc ggattttgag agccgcgaac agtggaaaga ggcagccgat atgtatgccc
     1261 gtctggtcaa ggcctacccc aagtcaaaac gcgctgatga aaccctctac cggctcggcg
     1321 tgctctatgc gaacaacctc aaggactatc aaaagtccgt tgatgcctac aggcagttga
     1381 tcagcaaata ccccaagagc aattatgtca tccaatccag tttcatgatc ggttatcgtt
     1441 atgccaatga catcaaggat tttgataatg ccaaaaaata ttatcaggag tttttgaaga
     1501 aatggcccgc acatgaattg gcctcttcgg tgaaatggga actggaccat ttgggcaagg
     1561 acatcagcga gctcgagctt cagcttggca atcccgcccc ggatgctgct gaacaaaaat
     1621 aacgcaacct gatatttctc tctgtgccgc gccctgcggc acagagaaat tcgccgatcg
     1681 aatggccaca ccacctcttc agccctttta tcggtttttt gttgcctgga ggagttgaat
     1741 gcgcataatc cgtatcgccg cggccatagc cattttcttt tcttcgctcg ggatcagctc
     1801 cctgcaggcg cagggggagg atgagggcat tcgggtctcc gcttcggccc aacccaatga
     1861 ggtgcccctg aaccgcacgg cggttgtcac catccgcctg gagtgggcag gtgatctcga
     1921 ccgctatgaa atcacccgct ttgacaatcc tctggtcgag aacctcaaca ttatcgaaac
     1981 tgccagtgcc aaccaggttc aggtcgtcgg cggcctgcaa gtcgcccggc aggagtatca
     2041 gtacaccgtc aagcccgacg gtctcggcat ggcctatgtc gacggcatca tcatccgcta
     2101 cgccgaccgc gagaccggca aggagtaccg tctcgccagc agccggctgg agctcaaggt
     2161 ggtcgatcct ctgcccgagc cgggttctta tctctggctg gttattctgc tctccatact
     2221 gctctcctcg ctgatcgcca tctatctggt gcggcggatc cgtgccaggc gcgccgaggc
     2281 tgagcgtctc gcccgcagtc aggcggccag tgaagtgagc cttgaacaga aatacggtga
     2341 acggctgagg gaagagatcg atcttcgcca gcccgatctc gatgtcaatg ccgccttcgg
     2401 ccgtcttgct ggggtgctgc gccgttatct ggccgcaaaa tacagcctgc cgctgatcag
     2461 cgcgaccacc tccgaagtgc gcgggttaat ggccgaggcc ggtgccgatg agcgtgtgat
     2521 cgccgatact ctcgccgtcc tggagagcgc ggatatcgcc aagttttccg gcgccgcgca
     2581 gatgcgcagc gaactcgagc gcgcctacac gattctggag gggatgctcg gtacagatac
     2641 agccccgcgc ggaggcgcgg agagcgcaaa gagagatgac tagtcaaatt tgaaatttgc
     2701 tgtattcttt
//
LOCUS       DAOPWU010000423         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_39243_length_2704_cov_5.479049, whole genome shotgun sequence.
ACCESSION   DAOPWU010000423 DAOPWU010000000
VERSION     DAOPWU010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39243_length_2704_cov_5.479049"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..2518)
                     /locus_tag="PKM23_14600"
     CDS             complement(179..2518)
                     /locus_tag="PKM23_14600"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNY92751.1"
                     /translation="MKRMIVLTSLILSASAGAQAQTATLEATLELRTIDGVTVPYQNG
                     IPVPAFEKQQRTMIPLSGPWRKERFYANDALTLSSRAAAMPALLNEAKGREGRDFDDS
                     AWAVKSLPAVENQMMSYEAQPEFYEDGVWYRRTFEVPDSLQGRYIKLIFYAVNYVADI
                     WVNGQYLGWHEGGYTPFAFDVTPELEYGKTNTIALRVDNPAWGTRKDIVPFYRVDWFN
                     YTGVIHDLYLEASDPIHVVRSEVVPQTTAGEILVRTVVSNQGEAERSANVQIEIFRAD
                     VTAANQMSEQAAELTGAPAAFSGSAEASLTLPGYGSRVWQTSLHIADPALWTPKTPNL
                     YILRATVRRDGLIIDRFHTQFGIRTVVVDGNKVRLNHKPVFFTGVARHEDHPLYGRSI
                     PVAEIFKDLQGIKKNINCQFLRTGHYPNHLATYLFADRLGLTIMEEIPVWWYDDPEPW
                     LIQNEQRHIHTQMMREMIFRDYNRPSILLWSTCNECRDVDNRKIFIEGVNNELDTLYP
                     DGRLVSQSAAADRPSTRYPGTDASQRVCDVMGWTMYFGIFHGGTPGPGTAAFLDSVAS
                     QLPGKPALCTEYGYWSTPTGSSESTQLKVMRETFTSLEARGAVNATGSVREQGMLMAA
                     TWWCVYDWYSSSQGAQGVKEFQTMGLYHMDRATKKQVTPVLIQRYLPYTIHNGTTVGV
                     EEEPGAAALPEKMHLAANYPNPFNSRTVIRYELSQAAEVRLDILNLRGEVVETLVTGR
                     QPAGSHEAVWQSRAHASGLYLVRLQGAGGMQLRKMTLLK"
BASE COUNT          437 a          758 c          993 g          516 t
ORIGIN      
        1 gaggtgctgg gcgatggtgc ggttgaaatc aaagcggccc ggagcgggcg ccgaagaggt
       61 gtcatatcgg atgggcaaaa tagttacctc gtcataaaaa gcaaggcccg aatttcgggc
      121 ctcgatgatg cctggacccc gctgccggcc gtgtgccggc gcaggggaga ggctgtggtt
      181 acttgagcag ggtcatcttg cgcagctgca tcccgccggc gccctgcagg cggaccagat
      241 agagcccgct ggcgtgggcg cggctctgcc agaccgcctc gtggcttccc gccggctggc
      301 ggcccgtcac cagggtctcg accacctcgc cgcgcaggtt gaggatgtcg aggcgcacct
      361 ccgcagcctg gctcagctcg tagcgaatga ccgtccggct gttgaaggga ttgggatagt
      421 tggccgccag gtgcatcttt tccggcaggg ccgccgctcc gggctcctcc tccacgccaa
      481 cggtggtacc gttgtgaatg gtgtagggca gatagcgctg gatcagcacc ggggtcacct
      541 gctttttggt ggcgcggtcc atgtgataga gccccatggt ctgaaactcc ttgaccccct
      601 gggcgccctg cgagctggag taccagtcgt aaacgcacca ccaggtggcg gccatgagca
      661 tgccctgttc gcgcaccgac ccggtggcgt tgaccgcgcc gcgcgcctcg agggaggtga
      721 aggtctcgcg catcaccttg agctgggtgg actcggagct gccggtgggg gtggaccagt
      781 agccgtactc ggtgcagagg gccggcttgc cgggcagctg tgaggcgacg ctgtcgagaa
      841 aggcggcggt gccggggccc ggggtgccgc cgtgaaagat gccgaaatac atggtccagc
      901 ccatcacgtc gcagacgcgc tgggaggcgt cggtgccggg gtagcgggtg ctgggccggt
      961 cggccgcggc cgactggctg acaaggcggc cgtcggggta gagggtgtcg agttcattat
     1021 tgacgccctc gatgaagatc ttgcggttgt ccacatcccg gcattcgttg caggtgctcc
     1081 agagcaggat gctcgggcgg ttgtaatcgc ggaagatcat ctcgcgcatc atctgggtgt
     1141 ggatatggcg ctgctcgttc tggatcagcc agggctcggg gtcgtcgtac caccagaccg
     1201 gaatctcctc catgatggtg aggccgaggc ggtcggcgaa aagataggtg gcgaggtgat
     1261 tgggatagtg gccggtgcgc aggaactggc agttgatgtt ctttttgatc ccctgcaggt
     1321 ccttaaagat ctccgcgacc ggtatgctgc ggccgtagag ggggtgatcc tcgtggcggg
     1381 cgacgccggt gaaaaagacc ggtttgtggt tgaggcgcac cttgttgccg tcgacgacca
     1441 cggtgcggat gccgaactgg gtgtggaacc ggtcgatgat gaggccgtcg cggcgcacgg
     1501 tggcgcgcag gatgtagagg ttgggggttt tgggcgtcca cagggcggga tcggcgatgt
     1561 gcaggctggt ctgccagacc cggctgccat agccggggag ggtgaggctt gcctccgcgc
     1621 tgccggagaa ggcggccggg gcgccggtca gctcggcggc ctgctcgctc atctggttgg
     1681 cggccgtgac atccgcccgg aagatttcga tctgcacgtt cgcgctgcgc tcagcctcgc
     1741 cctggttact gaccacggtg cgcacgagga tttccccggc ggtcgtctgc ggcaccacct
     1801 cgctgcgcac cacatgtatc ggatcgctcg cttcgaggta gaggtcgtgg atcaccccgg
     1861 tatagttgaa ccagtccacc ctgtaaaaag ggacgatgtc cttgcgcgtg ccccaggccg
     1921 ggttgtcgac gcggagggcg atggtgttgg ttttgccgta ctccagctcc ggcgtgacat
     1981 cgaaggcgaa gggggtatag ccgccctcgt gccagccgag gtactggccg ttgacccaga
     2041 tgtcggcgac atagttgaca gcgtaaaaga tcagcttgat gtagcggcct tgcaggctgt
     2101 ccgggacctc gaaggtgcgg cggtaccaga ccccgtcctc gtagaattcg ggttgggctt
     2161 catagctcat catctggttt tcgaccgccg gcaggctctt caccgcccag gcgctgtcgt
     2221 cgaaatcgcg gccctcgcgg cctttggcct cattgaggag ggcgggcatg gcggcggcgc
     2281 ggctgctcag ggtgagggcg tcgttggcgt aaaagcgctc cttgcgccag gggccgctca
     2341 gggggatcat ggtgcgctgc tgtttctcaa aagccggcac ggggatgccg ttctggtatg
     2401 ggacggtgac gccgtcgatg gtgcgcagct cgagggtggc ctcgagggtg gcggtctggg
     2461 cctgcgcgcc cgcgctggcg gaaaggatca ggctggtcag gacgatcatt cgtttcatgg
     2521 atgggccctt tgcagtgtat gatggcggtg cgaggatgac agcgataata taagcgattt
     2581 ggaggagaag aaaaaggaaa atgcgcgggg aggtgccggg ctggacttaa atctcgtccc
     2641 catcgaggaa gacggccggg tgggcaccag agggtcgggg caggcgggcc ccccttcaat
     2701 ctcg
//
LOCUS       DAOPWU010000424         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_39301_length_2701_cov_5.473167, whole genome shotgun sequence.
ACCESSION   DAOPWU010000424 DAOPWU010000000
VERSION     DAOPWU010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39301_length_2701_cov_5.473167"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PKM23_14605"
     CDS             complement(<1..731)
                     /locus_tag="PKM23_14605"
                     /inference="COORDINATES: protein motif:HMM:NF028300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM35 domain-containing protein"
                     /protein_id="HNY92752.1"
                     /translation="MSRRILLVLILGIFTAVGAQTDSLVIQEQELGLCTWNGTIVTSS
                     GSISGWTGPGFIDVENGIGKAVSWEIYVQAEGTFLFTWRCAFGGTATNLRDARLLIDG
                     NSAVDSLLFPYTTTWSNWAEITPVPVHLSAGDHKIRLEALCSGGLANLDYLKILGSGL
                     SAWECSPQYTLTVGSNDTSRGSVSYGPVQALYDKGTMITLRARARPGFFFQSWTGDEP
                     GADSLHSFAIRSDVTAVARFLPEAV"
     gene            complement(786..>2701)
                     /locus_tag="PKM23_14610"
     CDS             complement(786..>2701)
                     /locus_tag="PKM23_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HNY92753.1"
                     /translation="RSARRHGTVVAGTPASGKVIASLGLEQKLAGLGEEGYLIVSTLY
                     RGKRITAIAASHDHGVLYGVFHFLRLLQTGQEITGLEIKSRPRIGVRLLNHWDNLDRT
                     VERGYAGRSLWDWEELPDRLDPRYIEYARANASIGINGTVLTNVNANSQVLTDGYLRK
                     VAALADLFRPYGIRVYLTARFSAPVEIGGLVTADPLAPAVIAWWQAKAEEIYRLIPDF
                     GGFLVKANSEGQPGPQNYGRSHADGANLLADALAPHSGIVMWRAFVYDNNVPDDRAKQ
                     AWNEFVPLDGRFRENALIQVKNGPIDFQPREPFHPLFGAMPRTAMMPEFQITQEYLGA
                     SVHLVYLAPLYEECLRSDTHAAGEGSTVARVVDGSLGGHTLTAMAGVSNIGDVRNWTG
                     HPFGQANWYAFGRLAWDPGLSSATIAGEWVRMTFTRDEEAAHTISGMMMASREIAVNY
                     MTPLGLHHIMYYGHHYGPGPWVDSGRADWTSVYYHRADSAGIGFERTPAGSNALEQYR
                     PPLRELYGRVESCPEELLLWFHHLPWEHRMKSGRILWEELCYRYDAGVRSVGRLRSEW
                     ASLQEHIDAERFSEVSTLLAKQERDARIWRDACTLYFQTFSGKPFPADFDPPQHDLQW
                     YKAHTYEDIPGIE"
BASE COUNT          497 a          831 c          905 g          468 t
ORIGIN      
        1 aacaccgcct ccggtaaaaa gcgcgccacc gcggtgacgt cgctcctgat cgcgaaggaa
       61 tgcaggctgt cggccccggg ctcgtcgccc gtccagctct ggaaaaagaa gccgggccgg
      121 gcccgggcgc gcagggtgat catcgtcccc ttgtcgtaaa gggcctgcac cgggccatag
      181 ctcaccgagc cgcgcgaggt atcgttactg ccgaccgtga gggtgtactg cggggagcac
      241 tcccaggcgc tgaggccgct gccgaggatc ttgagatagt cgagattggc gaggccgccg
      301 ctgcagagcg cctccaggcg gatcttgtgg tcgccggcgg agagatggac cggaaccggt
      361 gtgatctccg cccagttcga ccaggtggtg gtatagggaa aaagcagcga gtcgacggcc
      421 gaattaccgt caatgaggag ccgggcatcg cgcagattgg tcgcggtccc gccaaaggca
      481 cagcgccagg tgaaaagaaa ggtgccttcg gcctggacgt agatttccca gctcaccgcc
      541 ttgccgatgc cgttctcgac atcgataaag cctgggccgg tccagccgct gatgctcccc
      601 gacgaggtga cgatggtccc gttccaggtg cacaaaccca gctcctgctc ctgaatgacc
      661 agggaatcgg tttgggcccc gacggcggta aaaattccaa ggatcaggac cagcaaaatc
      721 cttctgctca tgactgctct ccgcgcctgt tcagccggcg gttcgggtga caacaggccc
      781 cggtttcact cgatgccggg gatatcctca taggtgtgcg ccttgtacca ctgcagatcg
      841 tgctgcggcg ggtcaaaatc cgccgggaag ggtttaccgg aaaaggtctg aaaatagagg
      901 gtgcaggcgt cgcgccagat gcgggcgtcc cgctcctgtt tggcaagcag agtgctgacc
      961 tcggaaaagc gttcggcatc gatgtgctcc tgaagactcg cccactccga ccgcaggcgg
     1021 ccgaccgacc tgacgccggc gtcatagcga tagcaaagtt cttcccagag tatccgcccc
     1081 gatttcatgc ggtgttccca ggggagatgg tgaaaccaga gcagcagctc ctcggggcag
     1141 ctctcgacgc ggccgtagag ctcacgcagc ggcgggcggt actgctcgag ggcgttgctg
     1201 cccgcgggag tgcgctcgaa gccgatcccg gcgctgtcgg cgcgatggta ataaaccgag
     1261 gtccagtccg ccctgccgct gtcgacccag gggcccgggc cgtagtggtg gccgtagtac
     1321 atgatatgat gcagccccag gggtgtcata taattaaccg cgatctcgcg cgaggccatc
     1381 atcatgccgc tgatggtgtg ggcggcctcc tcgtcccggg tgaaggtcat cctcacccat
     1441 tccccggcga tcgtcgccga ggagaggccg gggtcccagg cgagccgccc gaaggcgtac
     1501 cagttggcct gaccgaaggg atggccggtc cagttgcgta catccccgat gttagagact
     1561 cccgccatag ccgtgagggt gtgaccaccg agtgaaccat cgaccacccg cgccacggtc
     1621 gagccctcgc cggcggcatg ggtgtccgag cgcaaacact cctcataaag cggcgccagg
     1681 tagaccagat ggacggaggc gccaagatac tcctgggtga tctggaattc gggcatcatc
     1741 gccgtgcggg gcatcgcccc gaagagggga tgaaagggtt cgcgcggctg gaaatcgatc
     1801 ggcccgttct tgacctggat gagggcattt tcacggaagc ggccgtcgag ggggacgaac
     1861 tcattccagg cctgtttggc gcggtcgtcg gggacgttgt tgtcatagac gaaggcccgc
     1921 cacatcacga tcccgctgtg gggcgccagg gcatcggcga gcaggttggc gccgtcggcg
     1981 tggctgcggc cgtaattctg gggacccggc tgcccctcgg agttggcctt gacgagaaaa
     2041 ccgccgaaat cgggaatcag ccggtagatc tcctccgcct tcgcctgcca ccaggcgatg
     2101 acagccggcg cgaggggatc ggcggtcacc agcccgccga tctcgaccgg ggcgctgaat
     2161 cgcgcggtca agtagacccg gattccatag ggacggaaaa gatccgccag cgcggccacc
     2221 ttgcgcagat agccatcggt cagcacctgc gagttggcat tgacgttggt caggactgtc
     2281 ccgttgatcc cgatcgaggc gttggcccgg gcgtattcga tgtagcgcgg atcgaggcgg
     2341 tcgggcagct cctcccaatc ccagagggag cggcccgcat agccgcgttc gacggtgcgg
     2401 tcgaggttat cccagtggtt gaggagtctc accccgatcc tgggccggct tttaatctcc
     2461 aggccggtga tctcctgccc cgtctgcaga aggcgaagga aatgaaagac gccatagagt
     2521 acgccgtggt cgtggctggc ggcgatggcg gtgatccttt ttcccctgta aagggtgctg
     2581 acgatcagat agccttcctc gccaaggccg gcgagcttct gctccagccc gagggaggcg
     2641 atcaccttgc cggatgccgg cgtgccggcc acgaccgtgc catggcgtct ggcggaacgc
     2701 g
//
LOCUS       DAOPWU010000425         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_39396_length_2696_cov_4.299886, whole genome shotgun sequence.
ACCESSION   DAOPWU010000425 DAOPWU010000000
VERSION     DAOPWU010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39396_length_2696_cov_4.299886"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="PKM23_14615"
     CDS             <1..1604
                     /locus_tag="PKM23_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HNY92754.1"
                     /translation="VDAMRGAKMYEISVRSIIAPNAVQTPLMLKSREGFYINLFEAGL
                     VNYPAMNLLIDQRTFSLTAALVPDALGNMAYLQTPEKSPWRTVIVSRDAREILASRII
                     LNLNEPCALTETEWIRPQKYIGIWWGMHAGTASWNMADTSNLKLVSTDWNALKPNGRH
                     GATTAETKRYLDFAARHGFDGVLVEGWNIGWEDWFGNWKEEVFDFVTPYPDFDVEALQ
                     HYAEERGVRLIMHHETSAAVTNYERRMQEAFAFMKRHGYHTVKTGYVGRIIPRGEHHD
                     GQWMVNHYLRVAREAARHEIMIDAHEPVRPTGLHRTWPNWLACEAGRGNEYNAWSSGN
                     PPDHETILPFTRLMGGPMDYTPGIFQIRMEYYKPGSTFQVHTTLAKQLALYVTLYSPL
                     QMAADLIENYDRFPDAFQFIKDVAVDWEESRIIAAEPGDYLTIARKAKDSPNWFVGAI
                     TDEQARSATVALDFLDPGTTYIATLYLDSKEAHWQNNPMAYRIQKGLVTAKSVLKMAL
                     APGGGAAVSIMPAAASEMKALRKLK"
     gene            1626..2264
                     /locus_tag="PKM23_14620"
     CDS             1626..2264
                     /locus_tag="PKM23_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92755.1"
                     /translation="MRFGAAAASALIILLAIFRPVRAQEAAAPTGGTGEGREALEQAR
                     LLFYASVEQKKQIGPAIAAFEELAGNYPAWAGRAKTYIGALVALQGKHAFLPHDKWRL
                     ANRGLAIMDEGVAMAPEDVESLFIHSSTCYFLPFFFKRGEDAQAKFRELVRLLPEKHR
                     EFDEPMLANVVQFIAERARLDENEKAALERLRSELTLPGTGPGGGAHEEAGS"
     gene            2261..>2696
                     /locus_tag="PKM23_14625"
     CDS             2261..>2696
                     /locus_tag="PKM23_14625"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNY92756.1"
                     /translation="MSHLEYLLFNLLVAAGPVALSFEPKVRFLRRWPAALLAALVMLA
                     PFALWDSLVTGRHWWFNPRFTAGTFIGALPAGEWLFFITVPFSSLFVWEVLRFYRPRG
                     HADSGRRPPLWLWAALPLAGLLLWLGKEYTALVIAAFALTAWA"
BASE COUNT          493 a          902 c          804 g          497 t
ORIGIN      
        1 aggtcgacgc catgcgcggg gccaaaatgt acgaaatctc ggtgcgctct atcatcgccc
       61 ccaacgccgt gcaaaccccc ctgatgctca agagccgtga gggattttat atcaaccttt
      121 tcgaggcggg cctggtcaac tatccggcga tgaatctgct catcgaccag cgcacctttt
      181 cgctgacggc agcgctggtg cccgatgccc tgggcaacat ggcctacctg cagaccccgg
      241 aaaagagccc ctggcgcact gtcattgtca gccgcgacgc ccgtgagatc ctcgcctcgc
      301 gaatcatcct caacctgaac gaaccctgcg ccctgaccga aactgaatgg atcaggccgc
      361 agaaatacat cggcatctgg tggggcatgc acgccggcac cgcctcctgg aatatggccg
      421 acaccagcaa tctcaagctc gtctccaccg actggaacgc cctcaagccc aacggccgcc
      481 acggcgccac cacggccgag accaaacgct acctcgattt cgccgcccgg catggctttg
      541 acggcgtgct ggtggagggc tggaacatcg gctgggagga ctggttcggc aactggaaag
      601 aggaggtctt tgatttcgtc accccctatc ccgatttcga cgtcgaggcg ctgcagcact
      661 acgccgagga gcgcggggtg cgtttgatca tgcatcacga gacctcggcg gcagtgacca
      721 actacgagcg gcggatgcag gaggcctttg cctttatgaa aaggcacggc taccacaccg
      781 tcaagaccgg ctatgtcggc cgtatcatcc cgcgcggtga gcaccacgac ggccagtgga
      841 tggtcaacca ttatctgcgc gtcgcccggg aggcggcgcg ccatgagatc atgatcgatg
      901 cccacgagcc ggtgcggccg accgggctgc accgcacctg gccgaactgg ctggcctgcg
      961 aggcggggcg cggcaatgag tacaacgcct ggagcagcgg caatccccct gaccacgaga
     1021 ccatcctccc cttcaccagg ctcatgggcg gtccgatgga ttacaccccc ggcatttttc
     1081 agatcaggat ggagtattac aagccgggca gcacttttca ggttcacacc accctggcca
     1141 agcagctcgc cctctacgtc accctctaca gccccctgca gatggcggct gacctgatcg
     1201 agaattacga ccgcttcccc gacgcctttc agtttatcaa ggatgtggcg gttgactggg
     1261 aggagagccg catcatcgcc gcggagccgg gcgactatct gaccatcgcc cgcaaggcga
     1321 aagattcgcc gaactggttc gtcggcgcca tcactgatga gcaggcccgc tctgcgaccg
     1381 tcgccctcga ttttctcgat cccgggacca cttacatagc aaccctctat ctcgacagca
     1441 aggaggccca ctggcagaat aaccccatgg cctaccggat tcaaaagggg ctggtgaccg
     1501 caaaatcggt gctcaaaatg gctctggccc ccggcggcgg ggcggcggtg agcatcatgc
     1561 cggcagccgc ctccgagatg aaagcactca gaaaactgaa atagcagtgg aaggaaaatc
     1621 cccccatgcg ctttggagcg gcggcagcgt ccgcactcat cattctcctg gccattttta
     1681 gaccggtgcg cgctcaggag gctgcagcgc caaccggtgg aacaggggag gggcgggagg
     1741 ctcttgagca agcgcgcctg ctcttttacg ccagcgtgga acagaagaaa cagatcgggc
     1801 ccgccatcgc cgcctttgag gagctcgccg ggaactatcc cgcgtgggcg ggacgggcga
     1861 agacctacat cggcgccctg gtcgctctgc agggcaagca tgcctttctg ccccacgaca
     1921 agtggcgact ggccaaccgt ggactcgcga tcatggatga gggggtcgcc atggctcccg
     1981 aggatgtcga gtccctcttc atccacagct cgacctgcta ttttctcccc tttttcttca
     2041 agcgcggtga ggatgcccag gccaagttcc gcgagctggt gcgcctgctg ccggagaagc
     2101 accgcgagtt cgacgaaccc atgctcgcca atgtggtaca gttcatcgcc gagcgcgccc
     2161 gcctcgatga gaacgaaaaa gcggctctgg agcgcctccg gtcggagttg acgcttcccg
     2221 gtaccgggcc tggcggcgga gcgcacgagg aggccggctc ttgagccatc tggagtatct
     2281 ccttttcaac ctgctggtcg ccgccggccc tgtggcattg agcttcgaac cgaaagtcag
     2341 gtttctgcgc cgctggcccg ccgccctgct cgccgccctt gtcatgctgg cgcctttcgc
     2401 cctctgggat tccctggtca ccggccgcca ctggtggttc aatccccgct tcaccgccgg
     2461 gaccttcatc ggagcccttc cggccgggga gtggctcttt ttcatcacgg ttcccttctc
     2521 ctctctcttc gtctgggagg tgctgcgctt ctaccgcccg cgcgggcatg cggattcggg
     2581 gcggcgtccc ccgctctggc tctgggcagc cctgcccctc gccggcctgc tgctctggct
     2641 gggcaaggag tacaccgccc tggtgatcgc tgcctttgcc ttgacggcct gggccg
//
LOCUS       DAOPWU010000426         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_39558_length_2688_cov_4.006077, whole genome shotgun sequence.
ACCESSION   DAOPWU010000426 DAOPWU010000000
VERSION     DAOPWU010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39558_length_2688_cov_4.006077"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKM23_14630"
     CDS             <1..371
                     /locus_tag="PKM23_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92757.1"
                     /translation="EMILDPGIAPIASDMNGDLRRLVEAMTLIHAEEELAGVNMSLGL
                     SNFTQMLPSKKADGSPVKGPLESAFLTIAMPLGLNMVIGSVNRKYALLEEDHPAMQCV
                     REALTLEGFDVIMRVMAYYS"
     gene            complement(399..1886)
                     /locus_tag="PKM23_14635"
     CDS             complement(399..1886)
                     /locus_tag="PKM23_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNY92758.1"
                     /translation="MLKKISAVAWLVLFSLTPLSAQEENIRFLINQFGDEDPFVNGEA
                     ILALAAAGEQTVDSLLPILAKGDDNARRCAAIALHKIAPRGKQAIPALTGALNDSSSH
                     VRWCSALALAKFGADAADAAAELGKLFHDPDNNVRWAAYIALSAIDRVKLNQPPTVAE
                     VTGRIEKMAPKFMSTLHVPGVAIALIRESKTIWTGYFGLADASRGIKVDASTMFEVCS
                     MSKPVFAFLALKMVEEGVLDLDRPLYDYLPEEFIADDPDFGRMVTARRALAHCSGMPN
                     WRKGGEERIGPLPIYFRPGSRFGYSGEGIYYLQRVVERLCGEPLQELAQKQLFSKLGL
                     RSTSYIWREELDGQFSAGHDSAGQCLEASRYHHANASYTLRSTAEEYARLLTAIVRPA
                     QQENFLLSEEMRKEMLSHQIRVDSREVMDRPGRHLGLAGFRGLGWAVDSTITHDVAQH
                     TGSNQSGFRCYAQLDVEDGSGMVILTNALNGDELYRRLIAAIGDF"
     gene            2079..2417
                     /locus_tag="PKM23_14640"
     CDS             2079..2417
                     /locus_tag="PKM23_14640"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNY92759.1"
                     /translation="MPKMVAACGLDCFGCECREATLSGDMAKKADIAVRWSQQYSAAL
                     TAADINCVGCTEEGAHFGWCEQCPIRTCAVSKNYKTCAECVEFPCDKSRLILDAVPDA
                     KANIESLRAH"
     gene            2498..>2688
                     /locus_tag="PKM23_14645"
     CDS             2498..>2688
                     /locus_tag="PKM23_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92760.1"
                     /translation="MNDFDQAIRKIGMKNLVAGVFLCLVCGAGFAIKKESISAAVVPT
                     LLGIVLLIGGAISLAMKNS"
BASE COUNT          545 a          783 c          808 g          552 t
ORIGIN      
        1 cagaaatgat tctggatcca ggcattgcgc cgattgccag cgacatgaac ggggatctgc
       61 gccgtctggt cgaggctatg accctgatcc atgcggaaga ggagctggct ggcgtgaata
      121 tgtcgctcgg actgagcaat ttcacccaga tgctgccgtc taaaaaagcc gatggttcgc
      181 cggtcaaggg ccccctcgag agcgcctttc tgaccattgc gatgccgctg ggattgaata
      241 tggtcatcgg ttcggtcaac cggaaatacg ccttattgga ggaggaccat ccggccatgc
      301 aatgcgtgcg agaggccctg accctcgagg gcttcgatgt catcatgcgg gtgatggcct
      361 actattcgtg accggcggac ccgcaggcag cgagccagtt agaaatcgcc gatggccgcg
      421 atgaggcggc gataaagctc atccccgtta agggcgttgg tcaagatcac catgcccgag
      481 ccatcctcga catcgagctg tgcgtagcag cgaaaaccgc tctgattgga gccggtgtgc
      541 tgggcgacat catgggtgat ggtcgagtcg accgcccagc ccaggccgcg aaagccggcc
      601 agcccgagat gccggcctgg gcggtccatc acctcccggc tgtcgacgcg gatctgatgt
      661 gagagcatct cttttctcat ctcctccgag agaaggaaat tttcctgctg agcgggtctg
      721 acaatagccg tcagcagcct ggcatactcc tcagccgtgc tgcgcagggt gtaggaggcg
      781 ttggcatggt ggtagcggct cgcctcgagg cactgcccgg cgctgtcatg gccggctgag
      841 aattgtccat ccagctcctc acgccagata taacttgtcg aacgcagtcc cagcttgctg
      901 aacagctgct tttgtgccag ctcctgcagc ggctcgccgc acagccgctc gaccaccctc
      961 tgcagatagt aaatgccttc ccctgaatag ccaaacctgg atcccggccg gaaatagatg
     1021 ggcagcgggc cgattctctc ttctccaccc ttgcgccagt tgggcatgcc gctgcagtgg
     1081 gcgagagccc gcctcgctgt caccatgcgg ccgaaatcgg gatcatccgc gatgaactcc
     1141 tccggcagat agtcatataa aggtctgtcc agatccagca ccccctcctc gaccatcttg
     1201 agcgccagaa aggcaaaaac cggcttgctc atcgagcaga cttcgaacat ggtggaggcg
     1261 tcaaccttga tcccgcggct ggcatccgcc aggccgaaat agccggtcca gattgtcttg
     1321 ctctcccgga tgagcgcgat cgccaccccc ggtacatgca gtgtgctcat gaacttgggg
     1381 gccattttct caatccggcc ggtcacctcc gcgacggttg gcggctggtt cagcttgacc
     1441 cggtcgattg cggagagggc aatgtaggcg gcccagcgga cattgttgtc gggatcatgg
     1501 aagagcttgc ccagttccgc agcagcgtcg gcggcgtcag ctccaaattt tgccagggcg
     1561 agggcggaac accagcgcac atggctggag ctgtcgttca aggcaccggt caaggccggg
     1621 atggcctgtt tgcccctggg agcgatcttg tggagcgcga tggcggcgca gcggcgggcg
     1681 ttatcatccc ctttagccag gatcggcagc agagagtcca ccgtctgctc accggcagct
     1741 gccagggcca ggatggcctc tccgttcaca aagggatcct cgtcgccgaa ctggttgatc
     1801 aggaaacgga tattctcttc ctgcgctgaa agcggggtga gggagaagag cactagccat
     1861 gcgacagccg atattttttt taacatatca agcctctctg tgccagtaag ggttacgcag
     1921 cgcaatcatc aagggctggc gtcgaccggg accctgcttt ctccttgaca ttggcagcct
     1981 gattgtttat actaatctga ggcttccggc ggcagccctg ccatggctgc tgcggccggg
     2041 atggctcatg aacctgaaac aaagtcagga gtctgataat gcccaaaatg gttgctgcct
     2101 gcggactgga ttgcttcggt tgcgagtgcc gtgaagcgac cctctccggg gatatggcga
     2161 aaaaggcgga tatcgccgtg cgctggtcac agcagtacag cgccgctctg accgccgccg
     2221 acatcaactg cgtcggctgc acggaggagg gtgctcactt tggctggtgt gaacagtgcc
     2281 ccatccggac atgcgccgtt tcgaagaact acaagacctg cgcggaatgc gtcgaattcc
     2341 cctgcgataa aagccggctc atcctcgacg cggttcctga cgccaaagcc aatatcgaat
     2401 ccctgcgggc gcactaattc agtggcaaaa gcggcttgca gccctgctga aggcagaatg
     2461 atagggagat agtctccgat atttcaggga gaagatcatg aacgattttg atcaggccat
     2521 caggaaaatc ggtatgaaga atctcgtcgc cggcgtattt ctgtgcctgg tttgcggtgc
     2581 cggctttgcc atcaaaaagg agtcgatctc cgcagccgtt gtgcccacat tgctcggaat
     2641 tgtactcctg atcggcggcg cgatttctct ggcgatgaag aactccag
//
LOCUS       DAOPWU010000427         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_39755_length_2678_cov_4.899733, whole genome shotgun sequence.
ACCESSION   DAOPWU010000427 DAOPWU010000000
VERSION     DAOPWU010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39755_length_2678_cov_4.899733"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2678
                     /locus_tag="PKM23_14650"
     CDS             <1..>2678
                     /locus_tag="PKM23_14650"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNY92761.1"
                     /translation="DDNWGNIRKLPRPGEAPRSGGYGIYYHFDYVGGPRNYKWLNTNQ
                     VARIWEQMHLAYAAGARRIWIVNVGDIKPMELPTEFFLDYAWNPEAWPAERIPEYTRQ
                     WAARQFGAEHAAEIAALLEGYTRFNSRRKPELLEPATFSLNNYGEWERVLSDYNQLAE
                     RARRLNELLPPAQRDAFYQLVLHPVTACANLYELYYTVALNRVYASQGRAATNAMAER
                     AEKLFARDAEISAYYNHELAGGKWSHMMDQTHIGYTYWQQPDSNAMPEVKRRSVPAGA
                     EMGVAIEGSAQWWPDSQAEAVLPELDPYIRKSTHLEIFNRGRSPFTYSIQPGRNWLRI
                     SDASSAVEDEKRITVTVDWGQVPTGRHRVPITVSGPGARRVVVTLPLFKPDEPETLLA
                     GRFLESNGCVAIEAEHYTRAVNSPALYWKNIPELGRTLSGMTPFPVTAPAAAPGGESP
                     RLEYLLHLFSVGTVTVKALLSPTLDYHHAGGLRYAISFNDEAPRIVDMHEGMNWDKWV
                     SDNIVVKSTTHTISRPGLQVLRFWAVDPGVVLQRLVVETGEPGGSYLGPPESFATRTL
                     TSVQNPILGGFYPDPSLCRVGEFYYLVTSSFAYFPGIPIFKSRDLINWQQIGHVLDRP
                     EQLDLEGHGVSRGLFAPTIRHHDGLFYVVCTLVDRGGNFVVTAPAAEGPWSNPVWLPE
                     VDGIDPSLFFDEGGQAWLVYNSVAPQGKPLYEGHRTIRLRGFDCARLRVTGEEKILVN
                     GGVDLSRKPVWIEAPHLFHREGWYYLIAAEGGTSENHSEVVFRSRQVDGPYLPWQKNP
                     ILTQRHLNPGRPEPVTSAGHADFVETPAGDWWAVFLGCRPYPPVEQNYYNTGRETFLA
                     PVRWSEGWPVINPDHDLIQPTYPAPLPVSPAWSPFS"
BASE COUNT          484 a          926 c          787 g          481 t
ORIGIN      
        1 cgacgataac tggggcaaca tccgcaagct gccgcggccg ggcgaggccc cgcgcagcgg
       61 cggctatgga atttattacc acttcgacta tgtcggcggc ccgcgcaact acaaatggct
      121 caacaccaat caggtagcgc gcatctggga acagatgcat ctcgcctacg cagcgggggc
      181 gcggcgtatc tggatcgtca acgtcgggga tatcaaaccg atggagctgc cgaccgagtt
      241 cttcctcgat tacgcctgga atccggaggc ctggccggcc gagcgcattc cggaatacac
      301 ccggcagtgg gccgcgcgcc agttcggcgc agaacacgcc gccgaaatcg ccgccctcct
      361 cgagggctat acccgcttca acagccggcg caaacccgaa ctgctcgaac cggccacctt
      421 cagcctcaac aattacggcg agtgggagag ggtgctctcc gattataatc agctcgcgga
      481 gagggcccgg cgcctcaacg aactcctccc ccctgctcag cgcgacgcct tttatcagct
      541 tgtcctccac cccgtcaccg cctgcgccaa tctctacgag ctctattaca ccgtcgcctt
      601 gaaccgggtc tacgccagcc agggccgcgc cgcgaccaat gcgatggcgg aaagggccga
      661 aaagctcttc gcccgcgatg ccgagatcag cgcctattac aaccacgagc tggccggggg
      721 caagtggagc cacatgatgg accaaaccca catcggctac acctactggc agcagccgga
      781 ctctaacgcc atgcccgagg tgaaacggag gagtgtgccg gccggcgccg agatgggggt
      841 ggccatcgaa ggttccgccc agtggtggcc ggattcacag gcggaggcag tacttcccga
      901 actcgatccc tatatcagga aatcgactca ccttgagatc ttcaaccggg gccggtctcc
      961 cttcacctac tccatccagc cgggccggaa ttggctgcgc atcagcgacg ccagctcggc
     1021 tgtggaggat gaaaaacgca tcaccgtgac ggtggattgg gggcaggttc caaccgggcg
     1081 ccaccgggtg cccatcaccg tcagcggtcc cggcgcccgc cgcgtcgtgg tcaccctccc
     1141 gctcttcaag cctgacgaac cggaaaccct cctcgccgga cgtttcctcg agtccaacgg
     1201 atgtgtcgcc atcgaggcag agcactatac ccgcgccgtg aacagcccgg cactctactg
     1261 gaaaaacatc cccgagctgg gccgcaccct ctccggaatg accccctttc ccgtcaccgc
     1321 cccagccgca gcgccgggtg gcgagagtcc gcgcctcgag tacctgctcc atctcttcag
     1381 cgtcggcact gtgaccgtca aggccctgct ctcccccacc ctcgattatc accacgccgg
     1441 cgggctgcgt tacgccatct ccttcaacga cgaggccccc cgcatcgtcg acatgcacga
     1501 gggcatgaac tgggacaagt gggtgagcga caacattgtg gtcaaatcca cgactcacac
     1561 catctcccgg ccggggctgc aggtgctcag gttctgggcc gtcgatcccg gggtggtgct
     1621 gcagcggctg gtggtcgaga ccggcgagcc cgggggctcc tatctggggc cgcccgagag
     1681 ctttgcgacc cggaccctga ccagtgttca gaatccgatc ctcggcggct tttatcccga
     1741 tccgagcctc tgccgggttg gtgaattcta ctatctggtc acttcttctt ttgcctactt
     1801 tcccggcatc ccgatcttca aaagccggga cctgatcaac tggcagcaga tcggccatgt
     1861 cctggatcgt ccggagcagc tcgatctgga ggggcacggg gtttcgcgcg gtctcttcgc
     1921 cccgaccatt cgccatcacg acggcctctt ttatgtggtc tgcaccctgg tcgaccgcgg
     1981 cggtaatttc gtcgtcaccg ctccggcggc ggagggcccc tggagcaacc cggtctggct
     2041 gccggaggtg gacggcatcg atccctctct ctttttcgat gaaggggggc aggcctggct
     2101 tgtctacaac agcgtggcgc cgcagggcaa acccctctat gaaggccatc gcaccatccg
     2161 cttgcgcggc tttgactgcg ccaggctcag ggtcaccggc gaggaaaaaa tcctcgtcaa
     2221 tggcggggtt gatttgagcc ggaaaccggt ctggatcgag gcgccccacc tcttccacag
     2281 ggagggctgg tattatctca tcgccgccga gggggggaca agtgaaaacc acagcgaggt
     2341 ggtcttccgc agccggcagg tcgacgggcc ctatctgccc tggcagaaaa atccgatcct
     2401 gacccagcgc cacctcaatc ccggccggcc cgaaccggtc acttccgccg gtcatgccga
     2461 ttttgtcgag accccggcgg gcgactggtg ggcggtcttt ctcggctgcc gcccttatcc
     2521 cccggtggag caaaattact acaacaccgg ccgcgagacc ttcctggcgc cggtgcgctg
     2581 gagcgagggc tggccggtca tcaatccgga tcatgatctg atccagccga cttatcccgc
     2641 ccccctgccc gtttcgccgg cctggtctcc cttctccg
//
LOCUS       DAOPWU010000428         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_39945_length_2669_cov_4.052793, whole genome shotgun sequence.
ACCESSION   DAOPWU010000428 DAOPWU010000000
VERSION     DAOPWU010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39945_length_2669_cov_4.052793"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2410)
                     /gene="priA"
                     /locus_tag="PKM23_14655"
     CDS             complement(<1..2410)
                     /gene="priA"
                     /locus_tag="PKM23_14655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNY92762.1"
                     /translation="MQPENETKIFAEVVFPVPVDHAYTYSIPEKFRGDLLPGMRVLAE
                     FGARKSTGFVVGVNADAGRSGLKAIEEVLDPVPLFTPEVLDLARWIAHYYLCGWGEVL
                     KAALPSGIHKHSVKVVRLTHERPLELAALLEKRAPRQSQIVRQLAEKNPLPVRELTSR
                     ADAGSIDASLAKLRQAGFIRYELMLPRPRVGQKFENFVRTAEGWNEESLFTLATELRK
                     SAPRQAALLDYLCQHAGEEFGRGDLAREARVPPDVVSALETRGVLLRSRIVVQRDYYG
                     ELEVAPPPQLVLNPGQREALRIIGGELERREFATALIHGVTGSGKTQVYIEAIARVLA
                     EGGSAIVLVPEIALTPQMVRRFRAHFGRLVAVFHSRMSPGERYDSWRATWEGKHRIVI
                     GPRSAIFAPLADVRLIVVDEEHEPSYKQNDLTPRYHARDVAVMRAKLNRAVVVLGSAT
                     PALESYYNALSGKYRLISMPNRIDDVPMPQIAFVDMRSEPKIIGRTDPVILSRYLRQK
                     IDEKLSRGEQIILFQNRRGFATLFKCSSCGYVAECENCSISLTYHLRGRLLKCHYCGF
                     VRKAPEVCPKCGGLDLFFRGIGTQRIEEELKELFPGIKAVRMDLDTTRGRHAHDRILS
                     AFGRGEYQILLGTQMVAKGLDFPRVTLVGVISADSELHFPDFRAGERTFQLLTQVAGR
                     AGRKERLGEVVIQTFTPEHSALLFVRHHDYVNFYAAEVKEREPLGYPPFSRMTHILFK
                     GEEEAAVLRTANQFAACLRPDEAYRALGPAPSPLARLEGFYRYQMLLMSSKEGDAGGS
                     LTRAV"
     gene            2348..>2669
                     /locus_tag="PKM23_14660"
     CDS             2348..>2669
                     /locus_tag="PKM23_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92763.1"
                     /translation="MVDGDGKDYLGEYFGFVFGLQDDEGFCLSEQELRLGRRRTLSSP
                     ASPDQGKLAIQGLKIKKYSGEKRPLYLHEKSLANQPNFLYLMRYAPPQGGARQRWKNE
                     GEKHE"
BASE COUNT          492 a          794 c          932 g          451 t
ORIGIN      
        1 ggacggccct ggtcaggctt cctccggcat cgccctcctt gctgctcatc agcagcatct
       61 gatagcggta gaacccctcg agccgggcca ggggcgaggg cgccggtccg agcgcgcggt
      121 aggcctcatc cggccgcagg caggcggcga actggtttgc cgtgcgcagc acggcagctt
      181 cctcctcccc cttgaagagg atatgggtca tgcggctgaa tgggggatag ccgaggggtt
      241 cgcgctcttt aacttcggcg gcgtaaaagt tgacatagtc gtgatggcgg acgaagagga
      301 gggcggagtg ctccggggtg aaggtctgga tgaccacctc gccgaggcgc tccttgcggc
      361 cggcgcggcc ggccacctgg gtgaggagct ggaaggtgcg ctcgccggcg cgaaaatcgg
      421 gaaaatggag ctcggaatcg gccgagatca cccccaccag ggtgacgcgg ggaaagtcga
      481 gccccttggc caccatctgg gtgccgagga ggatctggta ttccccgcgg ccgaaggcgc
      541 tgaggatgcg gtcgtgggca tggcggccgc gggtggtgtc gagatccatg cgcaccgcct
      601 tgatcccggg gaaaagctcc ttgagttcct cctcgatgcg ctgggtgccg atgccgcgga
      661 aaaagagatc caggccgccg cacttcgggc agacctccgg ggccttgcgg acaaagccgc
      721 agtagtggca tttgagcagc cggccgcgga ggtgataggt gagcgagatc gaacagttct
      781 cgcattcagc gacgtagccg cagctgctgc acttgaagag agtggcgaat ccgcgccggt
      841 tctgaaaaag aatgatctgc tcgccgcgcg acagcttttc gtcgatcttc tggcgcaggt
      901 agcgggagag gatgaccgga tcggtccggc cgatgatttt gggctcgctg cgcatgtcga
      961 caaaagcgat ctggggcatg gggacgtcgt cgatgcggtt gggcatcgag atcaaccggt
     1021 acttgccgga gagggcgttg taatagctct cgagggcggg ggtggccgag cccagcacga
     1081 ccaccgcccg gttgagtttg gcgcgcatca ccgccacatc gcgggcgtgg tagcgcgggg
     1141 tgaggtcatt ctgtttgtag gagggttcgt gctcctcgtc gaccacgatg aggcggacat
     1201 cggcgagggg ggcgaaaatg gccgagcgcg gaccgatgac gatgcggtgc ttgccctccc
     1261 aggtggcgcg ccaggagtca tagcgctcgc ccggagacat ccggctgtga aagaccgcca
     1321 ccagccggcc gaaatgggcg cggaagcgcc gcaccatctg cggggtgagg gcgatttcgg
     1381 gcaccagcac gatggccgat ccgccctcgg cgaggacgcg ggcgatggcc tcgatataga
     1441 cctgggtctt gccgctgccg gtcacgccgt ggatgagggc ggtggcgaac tcccggcgct
     1501 cgagctcgcc cccgatgatg cgcagggcct cccgctggcc ggggttgagc accagctgcg
     1561 gcggcggagc gacctcgagc tcgccgtaat agtcgcgctg gaccacaatg cggctgcgca
     1621 gcagaacgcc gcgcgtctcc agggcggaga ccacatccgg cgggacgcgc gcctcgcgcg
     1681 ccagatcccc gcggccgaac tcctctcccg catgctggca gaggtaatcg agcagggcgg
     1741 cctggcgcgg agcactcttg cgcagctccg tggccagggt gaaaaggctc tcctcgttcc
     1801 agccctcagc agtccggaca aagttctcga atttctgccc gacccggggc cgcggcagca
     1861 tcaactcgta gcggataaag ccggcctggc gcagtttggc cagactggcg tcgatgctgc
     1921 cggcatcggc gcggctggtg agctcccgga ccggcagggg gttcttctca gcaagctgac
     1981 ggacaatctg cgactggcgc ggcgcccgct tctcaagcag tgcggccagc tccagaggcc
     2041 tctcatgggt taggcgcacc accttgaccg agtgcttgtg gatgcccgag ggcagggcgg
     2101 ccttgagcac ctccccccaa ccgcagagat agtagtgggc aatccagcgc gccagatcga
     2161 gcacctcggg ggtgaagaga gggaccggat cgagtacctc ctcgatcgcc ttcagcccgc
     2221 tgcggcctgc gtcagcgttc actccgacga caaagccggt cgatttgcgg gcgccgaact
     2281 ccgccaacac gcgcatcccc ggcaggagat cgccccggaa tttctccggg atgctgtagg
     2341 tgtaggcatg gtcgacgggg acgggaaaga ctacctcggc gaatattttg gtttcgtttt
     2401 cgggctgcaa gatgatgaag gcttttgttt gagtgaacag gaactgcggc tggggcggcg
     2461 acggacactc tcctcgcccg cctctcccga tcaaggcaaa ttagctattc aggggcttaa
     2521 aatcaagaag tattcagggg aaaaaaggcc gctctatctc catgaaaaaa gccttgcgaa
     2581 tcagcctaat tttttgtatc taatgcggta cgctccgccg cagggcgggg ccaggcagcg
     2641 atggaagaat gaaggagaaa agcatgaaa
//
LOCUS       DAOPWU010000429         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_40135_length_2661_cov_3.186109, whole genome shotgun sequence.
ACCESSION   DAOPWU010000429 DAOPWU010000000
VERSION     DAOPWU010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40135_length_2661_cov_3.186109"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="PKM23_14665"
     CDS             complement(<1..1790)
                     /locus_tag="PKM23_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNY92764.1"
                     /translation="MKNFIACILFALAGPVLAQAPWLNPELPAGERARRLVREMTLEE
                     KISQMQHSASAISRLGIPACNWWNEALHGVARAGIATVFPQAIGLGATWDEALVGRIA
                     AVIGDEARAKHHEFVRQGKRGIYQGLTFWSPNINIFRDPRWGRGQETYGEDPFLTGRL
                     GVAFVKGLQGDDPRYFKAIATPKHYAVHSGPEPERHGFDAIAGERDLRETYLPAFEMC
                     VREGGAQSIMCAYNRYEGEPCCSSTLLMTSILRREWGFDGYIVSDCGAISDILYGHKM
                     VPGMAAAAARAVKSGCDLSCGEEYGALAEAVREGLIAETEIDTAVERLFTARFRLGEF
                     DPPERVPYARIPYTINDRPEHDRLALEAACASLVLLKNEGNALPWPKTLKKIAVIGPN
                     ADNLEVLLGNYNGTPSHPVTPLQGIRDKLGAQASVTYARGCILAEGMAAGEKRSAAAL
                     LEEALQAARTAEAVVLCLGISPRLEGEEMEVQVPGFHGGDRTSLDLPAPQQELMERLA
                     ALDKPTVLVLLNGSALVVNGANARIPAILEAWYSGQRAGTAIADALFGDYNPGGRLPV
                     TFYKSAADLPPFHEYTMAGRTYRYFTGEPL"
     gene            1980..2444
                     /locus_tag="PKM23_14670"
     CDS             1980..2444
                     /locus_tag="PKM23_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92765.1"
                     /translation="MKYPLFLMALAALLIAGCDWIDEVIDPEKGLVEMTVSKLKAAPG
                     DEITITLFNRGEKTVYLEGCNQFYYSTKSDTGWVDHTMVVCVWEGYEVPVPAGSAHTQ
                     KFVVPMMAGTLRFYAPVYRGCKPDLPISQAECVTMQRYYSPDVVVYPPRVPR"
BASE COUNT          497 a          771 c          882 g          511 t
ORIGIN      
        1 tagaggggct cgccggtgaa ataacgatag gtacgcccgg ccatggtgta ctcgtggaag
       61 ggggggagat cggcggctga cttgtaaaag gtgacgggca ggcggccgcc gggattgtaa
      121 tccccgaaaa gggcgtcggc gatggcagtg ccggcgcgtt gtccggaata ccacgcctcg
      181 aggatggccg ggatcctcgc attcgcccca ttgaccacca gtgcgctgcc gttgaggagc
      241 accagcaccg tcggcttgtc aagggcggcg aggcgttcca tcagctcctg ctggggggcg
      301 ggcagatcca ggctggtgcg gtcgccgccg tgaaatcccg gcacctgcac ctccatctcc
      361 tcgccctcga ggcggggcga gatgccgagg cagagcacca ccgcctcggc cgtcctggcg
      421 gcctgcagcg cctcctccag cagagccgcg gcggagcgct tttcgccagc ggccatgccc
      481 tcggccagaa tgcaccccct ggcataagtt acactggctt gcgccccgag cttgtcgcgg
      541 atcccctgca ggggggtgac cggatgggag ggggtgccat tgtagttgcc aagcagcacc
      601 tcgaggttgt cggcattggg gccgatgacg gcgatctttt tcagggtttt gggccagggc
      661 agggcgttgc cttcgttctt gagcagcacc agggaggcgc aggcggcctc gagggcgagc
      721 cggtcatgtt ccgggcgatc gttgatggta tagggtatcc gggcgtaggg gacgcgctcc
      781 gggggatcga attccccgag ccggaagcgg gcggtgaaga ggcgttcgac agcagtgtcg
      841 atctccgttt cggcgatcag cccctcgcgc accgcctcgg ccagggcgcc gtactcctcg
      901 ccgcagctga ggtcgcagcc ggatttgacg gcgcgggccg ctgcggccgc catccccggc
      961 accattttgt ggccgtagag gatatcagag atggccccgc agtcggagac gatatagccg
     1021 tcgaaacccc actcccggcg caggatagag gtcatcagca gggtgctgct gcagcagggt
     1081 tcgccctcgt agcggttata ggcgcacatg atcgattggg ctcctccctc gcgtacgcac
     1141 atctcaaagg cggggaggta ggtttcgcgc aggtcgcgtt cgccggcgat ggcgtcgaag
     1201 ccgtggcgct ccggctcggg accgctgtgc acggcgtagt gtttgggagt ggcgatcgcc
     1261 ttgaagtagc gcggatcgtc gccctggagg cccttgacaa aggcgacgcc gaggcggccg
     1321 gtgaggaagg gatcctcgcc gtaggtctcc tggccgcggc cccagcgcgg atcgcggaag
     1381 atgttgatat tcggcgacca gaaggtgagc ccctggtaga tgccgcgctt gccctggcgg
     1441 acgaattcgt ggtgcttggc gcgagcctcg tcgccgatca ccgcggcgat gcggccaaca
     1501 agcgcttcat cccaggtggc cccgagcccg atagcctggg gaaagactgt ggcgatgccg
     1561 gcccgggcga ccccgtgcaa ggcctcgttc caccagttgc aggcggggat gccgaggcgg
     1621 gagatggctg aggcgctgtg ctgcatctgg gagatctttt cttccagggt catctcgcgg
     1681 accagccggc gggcgcgctc cccggcgggc aactcgggat tcaaccaggg ggcctgcgcc
     1741 aggacggggc cggcgagggc aaacaaaatg caggcgatga agtttttcat ttttcctcgc
     1801 aatttgattt tcaggacagg tagaaaaaag tacggaagat tatcatatat cacaagcaag
     1861 gaacagattg gtgccgttta cgtataatat attgacttgt atccctctgg cggttatatt
     1921 tctgcagctt aaattttaac gctgccggca atagccgggc ggcagtgcag gagtggccta
     1981 tgaaatatcc tttgttcctg atggctttag cagccctgct gatcgcaggc tgcgactgga
     2041 tcgatgaggt tatcgatccg gaaaaagggc tggtcgagat gacagtgagc aagctcaaag
     2101 cggcaccggg cgatgagatc accatcaccc ttttcaaccg tggtgagaaa acggtctatc
     2161 tggaagggtg caatcaattc tattacagca ccaaaagcga taccggctgg gtggatcata
     2221 ccatggtagt atgtgtttgg gaaggttatg aagtgccggt cccggccggg agcgctcaca
     2281 ctcaaaagtt cgtcgttccc atgatggccg gtacactccg cttctatgcg ccggtctatc
     2341 gcggctgcaa accggatctg cccatcagtc aggcggaatg cgtcaccatg caaaggtatt
     2401 attcaccaga tgtggtggtc tatcctcccc gcgtaccgag atgaaaaaag ggtgcaaggc
     2461 gccggcagga gagcacaatc ctgcagaata ggtaattcac ttttatgctc aacgtgcggg
     2521 agttcatcgg ccgcacttcc gatcagcggc cgggatcacc tcacatccgc gggatgatgc
     2581 cggctccctc ttctccaata tcgatcagat gccatccggc ggcatccccg ctgttgacca
     2641 ccggtatgcc ccttcccacc g
//
LOCUS       DAOPWU010000430         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_40167_length_2659_cov_4.519201, whole genome shotgun sequence.
ACCESSION   DAOPWU010000430 DAOPWU010000000
VERSION     DAOPWU010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40167_length_2659_cov_4.519201"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1202)
                     /gene="selD"
                     /locus_tag="PKM23_14675"
     CDS             complement(180..1202)
                     /gene="selD"
                     /locus_tag="PKM23_14675"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018977138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1173..1175),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNY92766.1"
                     /translation="MTETVKCAGUASKLAPSELAAALEKLPKPNDPRVLVGYNTADDA
                     GVIRLSDELALVQTVDFFTPIVDDPFTYGQISAANSLSDVYAMGGTPFSALNIVGFPK
                     DIFPMEVLAAILQGGQEKAAEAGVPILGGHTVSDQELKYGLAVTGLIHPDRILTNAEA
                     RPGDLLVLTKPIGTGTITTALKAGKGTPEMEAEVCGAMAALNRAAAEVCQRVGVHAVT
                     DITGFGLLGHALEMAEASGVGLVLRFDAVPFFAAAIATTAAGFVPGGTRANLLHVQPC
                     IDFLERITPEQRLLLSDPETSGGLLIAVAEEKAFELVRAGRDEGVRMAVIGEVIADHA
                     GRIEVV"
     gene            complement(1245..2282)
                     /locus_tag="PKM23_14680"
     CDS             complement(1245..2282)
                     /locus_tag="PKM23_14680"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HNY92767.1"
                     /translation="MAKDYSRRDFVRIGALGAAAAPVLAAQTTAGMPQTDVKSADAVH
                     RPVILCSRGEAWGRKVLEPAWQTWLEKRSMLDAVERGANVVELDPEDQSVGYGGWPNE
                     EGVVELDACVMAGPFHQCGAVAGLQNIKTPSSVARLVMERTDHILLVGAGAKQFALRF
                     GFKEEELLTEASRQGWLKWKAGLSKEDDWLEPGDKKIFRPTGTINVLGVDDRGDLFGI
                     TTTSGLAFKIPGRVGDSPIIGAGLYLDNEIGAAGATGRGEEVIRTCGSFYTVSKMAQG
                     LSPQAACEAACRRIIEVNGGKADFNVKFIAVNKAGETGCAQLKGSEGTHLSCISAQGL
                     QAQQGTIQMAK"
     gene            complement(2377..>2659)
                     /locus_tag="PKM23_14685"
     CDS             complement(2377..>2659)
                     /locus_tag="PKM23_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92768.1"
                     /translation="PIFKKFQEKLALLHNGGIQIFGVSSEFLCRIPLNLVSPDIMRHV
                     PQSLHSISRILAAWVMVNHATFLNMLFSPVDHYSLFIYIRVGPRLRGAA"
BASE COUNT          509 a          814 c          807 g          529 t
ORIGIN      
        1 tgaatatcgg ataaaaccaa aataattcaa agaacatttt tggacatacg aaaatgtcca
       61 aaattaaaac ttgactttca caagagtaac ttcgtgtctt ggcgagagcc ttgatttctc
      121 tttgccttgg tgagagtgtt gtcgtctctc agcgcctctg cgtgaggccg attttatccc
      181 tacaccacct cgatccggcc ggcatggtcg gcgatcacct ccccgatcac cgccattcgc
      241 actccctcat cacgcccggc gcgcaccagc tcgaaggcct tctcctccgc cacggcgatc
      301 agcagacctc ccgaggtctc gggatcgctg agcagcagcc gctgttcggg ggtgatgcgc
      361 tcgaggaaat cgatgcaggg ctgcacgtgg agcagattgg cgcgggtgcc gccagggaca
      421 aatcctgcgg cggtggtggc gatggcggcc gcgaaaaacg gcacggcgtc gaagcgcagc
      481 accagaccaa cgccactcgc ctcggccatc tcgagggcat ggccgagcag cccgaatccg
      541 gtgatatcgg tgaccgcgtg cacgccaacg cgctggcaga cctccgcggc cgcgcggttg
      601 agggccgcca tcgcgccgca gacctccgcc tccatctccg gcgtcccctt gcccgccttg
      661 agggcggtgg tgatcgtgcc ggtgccgatc ggcttggtca ggacgagcag gtcgcccggc
      721 cgcgcctcgg cgttggtgag aatgcggtcg ggatggatca gcccggtcac cgccaggccg
      781 tacttgagct cctggtcgct gacggtgtgg ccgccgagga tgggcacccc cgcctcggcc
      841 gccttctcct gtccaccctg caggatggcg gcgagcacct ccatcggaaa aatatccttg
      901 ggaaagccga caatgttgag ggcgctgaag ggggtccccc ccatggcgta gacatcgctg
      961 agcgagttgg ccgccgagat ctggccgtag gtgaaggggt cgtccacgat cggggtgaag
     1021 aaatcgacgg tctgcaccag ggccagctca tcggagaggc ggatgacacc ggcgtcgtcg
     1081 gccgtgttat agccgacgag aacgcgggga tcgttgggtt ttggcagctt ttccaaagct
     1141 gcggccaatt cacttggcgc gagtttggac gctcagccgg cgcatttgac cgtttcggtc
     1201 agccgccgat gggggtattc gttcactttc ttctgctccg ctgactattt cgccatctgt
     1261 atcgtgccct gctgcgcctg caagccctgc gccgatatac aggagagatg ggtgccttca
     1321 ctgcccttga gctgggcgca gccggtctcc cccgccttgt tgaccgcgat gaatttgaca
     1381 ttgaaatcgg ccttgccgcc gttgacctcg atgatgcgcc ggcaggccgc ctcgcaggcc
     1441 gcctgggggg agaggccctg ggccatcttg ctgaccgtat aaaagctgcc gcaggtgcgg
     1501 atcacctcct cgccgcggcc ggtggccccg gcggcgccga tctcgttgtc cagatagagt
     1561 ccggccccga tgatcggcga atccccgacc cggccgggga tcttgaaggc caggccgctg
     1621 gtggtggtga tgccaaagag gtcgccgcgg tcgtcgactc cgagcacatt gatggtcccg
     1681 gtcggacgga aaatcttctt atcacccggc tccagccagt catcctcctt cgagaggccg
     1741 gccttccact tgagccatcc ctgccgggaa gcttcggtca acagttcctc ttctttaaag
     1801 ccgaacctga gggcgaattg tttcgccccc gcgccgacga ggaggatgtg atcggtgcgt
     1861 tccatcacca ggcgtgccac cgagctgggg gttttgatat tctgcagtcc cgccacggcg
     1921 ccgcactggt gaaagggacc ggccatgacg caggcatcga gttcgaccac cccttcctcg
     1981 ttgggccagc cgccatagcc gaccgactgg tcttccggat cgagctcgac cacattggcc
     2041 cccctctcga ccgcgtcgag catgctgcgc ttttccagcc aggtctgcca ggccggctcg
     2101 aggaccttgc ggccccaggc ctcgccgcgg ctgcagagga tgaccgggcg gtggactgcg
     2161 tctgcggatt ttacatccgt ctgcggcata ccggccgtcg tctgggcggc cagcactggc
     2221 gcggcggcgg cgccgagcgc gccgatgcgg acaaaatccc ggcgggaata gtctttggcc
     2281 atcacaagct ccatgctgtg tacatagctg ccgtggctct ttgatttaaa aattaacata
     2341 cgaattaaaa cgggcaaaat caacaccgta aaaaattcaa gctgcccccc gcaatctggg
     2401 tcccacccgg atgtaaataa ataaggaata gtggtctacc ggcgaaaaga gcatatttaa
     2461 aaaggttgcg tggttgacca taacccaggc agcgagtatg cgggaaatac tatggaggga
     2521 ttggggtacg tggcgcataa tgtcaggtga aaccagattt agggggatcc tgcacaaaaa
     2581 ttcgcttgat accccaaaaa tctggatccc accgttatgt aataaagcaa gcttttcttg
     2641 gaatttttta aaaattgga
//
LOCUS       DAOPWU010000431         2654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_40265_length_2654_cov_4.150058, whole genome shotgun sequence.
ACCESSION   DAOPWU010000431 DAOPWU010000000
VERSION     DAOPWU010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40265_length_2654_cov_4.150058"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..630
                     /locus_tag="PKM23_14690"
     CDS             163..630
                     /locus_tag="PKM23_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92769.1"
                     /translation="MHKKGHIAPDLQNLQLNQVEALDRETTGGKGLAYYETALISRPA
                     VFHNHISGRVGNYVENYDVRITFHENEIAGSCPCQRSRKICKHIIALLYSWVNDGGEF
                     LDVEDVLTEVRAMKKEQLLVVIENIIRQNPEYAELFLKRQPPDWDEIETEIGG"
     gene            802..>2654
                     /locus_tag="PKM23_14695"
     CDS             802..>2654
                     /locus_tag="PKM23_14695"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNY92770.1"
                     /translation="MSPDLQHRVNFWIKVFSKYGRDQVIIHDTDHMNVIYDVVDLTEG
                     TGTRRSRFGRVDAARKEIRAALQRLNSITIPSDTLKLTLRERQIYSLWSFSDDPRRFA
                     KAAENIHLQYGVRELFTRGLQRSGAYLDTMQAIFRSHGLPEDICYLPHVESAFNPRAY
                     SKVGAAGIWQFTRSTGRLYLRIDYAIDERYDPYLATHAAARLMRHNHEQLGSWPLAIT
                     AYNHGTGGVARAKKQVDSDDLAEIIDEYDGRRFGFASKNFYAEFLAARELGKNHRSYF
                     GEVELEKPADHQIFELPSYITLDALARKFKLNKDLIAELNPALRRPILKSARRIPKGY
                     KLRLPLQPGVDPASLYAQLPAEEKYDEQVSDRYYVVEKGDNLGRIAREFNTTVETLMD
                     LNDISNPRRLRVGQLLELPGSARLAGGAGAAPSTQPPVLIPPAAVATPAAERDELPGS
                     SAAEVATAVPADTLPRADLLTLAREHAGRDGTLYGPAAPDAEGQQPPAPSNWRFEVDF
                     TEPAGSTITVQDQETIGHLSEWLGITPQRLRALNALGSGARLTVGRKITVVFSATTPV
                     EFHRRRLEFHRALQEDFFSNFRVDSTSTYTVKQGDAIWALCNGLFDAPFWLL"
BASE COUNT          512 a          905 c          750 g          487 t
ORIGIN      
        1 ctccccgtgg tcctgctttt cctcttcctg agccgctggc tcatctccgg cctcaccctc
       61 ggcagtgtca agggctgagc acgccgcaga gctttttccc ttgatattct gaccatagtt
      121 tcgtatcatg aaacgcgggg gaggaagctg aggagttgag tcatgcacaa aaagggacac
      181 atcgctcccg atttgcagaa tttgcagctg aaccaggtcg aggccctcga ccgtgagacc
      241 accggtggca agggactggc ctattatgag accgccctga tctcccgtcc cgccgtcttt
      301 cacaaccaca tctccgggcg cgtcggcaat tacgtggaaa attatgatgt ccgcatcacc
      361 ttccacgaga acgaaatcgc cggctcctgc ccctgccagc gctcccgcaa gatctgcaag
      421 catatcatcg ccctgctcta cagctgggtt aatgacggcg gggagtttct cgacgtcgag
      481 gatgttctca ccgaggtgcg cgccatgaaa aaggagcagc tcctcgtggt catcgagaac
      541 atcatccgcc agaatccgga gtatgccgaa ctctttctca agaggcagcc gcccgactgg
      601 gatgagatcg agaccgagat cggcggatag gagggggaca acgggcaggg tggacggagg
      661 gagtagggga gtttgcgcgc attcaggaca ggaagaaatc acaagatggt gcagtcaaga
      721 attggcaggt ttttttacac agcagcagcg ttgatgcttc tggggggttt tcagggggct
      781 ttcgcggatg atctttttcc cgtgtcaccg gatcttcagc accgggtcaa cttctggatc
      841 aaggttttca gcaaatacgg ccgggaccag gtgatcatcc atgataccga tcacatgaat
      901 gtcatctacg atgtagtcga cctcaccgag ggcacaggga cgcggcgctc ccgtttcggc
      961 cgggtggacg ccgcccgcaa ggagatccgc gccgccctcc agcggctgaa cagcatcacc
     1021 attccgagcg ataccctcaa gctgaccctg cgcgagcgcc agatctacag cctctggagc
     1081 ttcagcgatg atccacgccg tttcgccaag gcggctgaaa acatccatct gcagtacggc
     1141 gtgcgtgagc tcttcacccg ggggctgcag cgctccggcg cctatctgga caccatgcag
     1201 gcgatcttcc gctctcacgg cctgcctgag gatatctgct acctgcctca cgtcgaatcc
     1261 gccttcaatc cccgcgccta ctccaaggtg ggcgccgctg gcatctggca gttcacccgt
     1321 tcgaccggcc gcctttacct ccgcatcgat tacgccatcg acgaacgcta tgatccctat
     1381 ctggccacac atgccgcggc ccgcctgatg cgccacaacc acgagcagtt gggcagctgg
     1441 ccgttggcaa tcaccgcgta caatcacggc accggcgggg ttgcccgggc gaaaaagcag
     1501 gtcgacagcg atgatctggc cgaaatcatc gacgaatatg acggccggcg ctttggcttc
     1561 gcctccaaga atttttacgc cgaattcctc gccgcccgcg agctgggcaa gaaccaccgc
     1621 agctatttcg gcgaggtgga gctggaaaag ccggctgatc accagatctt cgagctgccc
     1681 agctacatca ccctcgacgc cctcgcccgc aagttcaagc tcaacaagga tctcatcgcc
     1741 gaactcaatc ccgccctgcg ccgcccgatc ctcaaatcgg cccgccgcat ccccaagggc
     1801 tacaagctgc gcctgccgct gcagccgggc gtcgatccgg ccagccttta tgcccaactc
     1861 cccgctgaag aaaagtacga cgaacaggtg tcggaccgct actatgtagt cgaaaagggg
     1921 gacaatctgg gccgcatcgc ccgcgagttc aacaccacgg tcgagaccct gatggacctt
     1981 aacgacatca gcaatccgcg ccgcctgcgc gtcggccagc tgctcgagct gcccggttcg
     2041 gcccgcctcg cgggtggggc cggcgccgct ccctccaccc agccgcccgt gctcattccg
     2101 ccggctgcgg tcgccacccc ggccgccgaa cgcgacgagc tgcccggcag cagtgcggcc
     2161 gaagtcgcga ccgcggtccc tgccgacact ctgccccggg ccgatctgct caccctggcc
     2221 cgcgagcatg ccggccggga tggcacgctc tacggacccg cggccccgga tgccgagggg
     2281 cagcagcccc cggcccccag caactggcgc ttcgaggtcg atttcacaga accggccggc
     2341 agcaccatca cggttcagga tcaggagacc atcggccatc tctccgaatg gctgggcatc
     2401 acgccgcagc ggctgcgcgc cctcaacgct ctcggctcgg gcgccaggct gacggtcggc
     2461 agaaagatca cggtggtttt ttccgccacc accccggtcg agttccaccg tcgccgcctc
     2521 gagttccacc gcgccctcca ggaggatttc ttttccaatt tccgagtcga cagcacctcc
     2581 acctataccg tcaagcaggg cgatgcgatc tgggccctct gcaacggcct cttcgatgct
     2641 cccttctggc tcct
//
LOCUS       DAOPWU010000432         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_40371_length_2649_cov_3.973400, whole genome shotgun sequence.
ACCESSION   DAOPWU010000432 DAOPWU010000000
VERSION     DAOPWU010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40371_length_2649_cov_3.973400"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /gene="coaE"
                     /locus_tag="PKM23_14700"
     CDS             complement(<1..529)
                     /gene="coaE"
                     /locus_tag="PKM23_14700"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNY92771.1"
                     /translation="MLRVGLTGVYGCGKSLAARTLHEEHAIPVIDMDLAGRAAAGRSS
                     VLRRLRTAFGPGIFDALGRLDRRALGRIVFADPGERRKLNAIVHPAMLAIVERQMRRC
                     AGGSAPYLIVDSALIFELGFEKKLDLVVAVHLPFDLILERAMARDGLSRTEVLERFAS
                     QLPQEEKCRRADYLLD"
     gene            complement(540..>2649)
                     /locus_tag="PKM23_14705"
     CDS             complement(540..>2649)
                     /locus_tag="PKM23_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNY92772.1"
                     /translation="RQLYTLPESAIPRSSGDTDGDGLPELLAGYGFSTWLYEAGGSEP
                     FRMNLVRKWQGDSRVQYWGSRLSDLDGDGRSELIMRVVRSGDSSSDLFEVWRSSGGGE
                     FETMAVLANPSAGDNFNGVPRCETGDFDGDGRIELLFGDSDGDIYLYEYDGSAFTPTW
                     QDRLPLLDAIDFIAAGDFDGDGENEFAAGSHSDPNLNTEHSYDSRHWCYRIYDTTGNN
                     TYLPAAEWRFFGYESARDFESGVSCGDADGDGRDELFIAAFPDLYVASFTPERGYELT
                     FHAAPIRTNAVMVADSDGDGLMEFWASDGERVRPRVLAESLTGPAMPVGVAARPLNAG
                     TVALSWNPVPGAQDYLLFRGLSADSLKLLQQTLLPVFTDTLVRAGEFYYYAVASRDTT
                     RLPAVSLRSEVVSARPGEQPALLSAAMAGARSVRLRFSRPMNSALLQDIRHYTIAPEV
                     GHPATALPLASGAEVLLSLEAPFTQSGFYSVGVRGLEDSDGLPVDTLRSQSEFEVAFA
                     AGAPYLTAVRAIASGVVELEFSEPMQKASLEEIANYDPGAGLHVAWAQAVAPENRCVR
                     LHLTGSVAWGAVGKPLTLRVSGLQSASGAAIKPGHGDTIQLLFAAADLDEVHTFPNPY
                     RLWIDPEGITFANLTPEAEIRIMTTEGRTLRILYEENGDGGLVWDVRDDQGRLVPAGI
                     YLYRVQASGMVRLGKLAVVR"
BASE COUNT          466 a          848 c          913 g          422 t
ORIGIN      
        1 tgtcgagaag gtaatcagcg cgccggcatt tttcctcctg cggcagctgg gaggcgaatc
       61 gttcaaggac ctcggtgcgg ctgaggccgt cgcgggccat ggcccgttcg aggatgaggt
      121 cgaatggcaa atggaccgcc accaccagat ccagcttttt ttcaaagccg agttcgaaga
      181 tgagggcgga atcgacgatc aggtatggtg cgctgccccc ggcgcagcgg cgcatctgcc
      241 tctcgacgat ggcgagcatg gccggatgga cgatggcgtt gagcttacgc ctctccccgg
      301 ggtcagcaaa gacgatgcgg ccgagggcgc ggcggtccag cctgcccagg gcgtcaaata
      361 tgccgggtcc gaaggcggtg cgcagccgcc gcagcacgct gctcctgccg gcggcggcgc
      421 ggccggccag gtccatatcg atgacgggga tggcgtgctc ctcatgcagg gtacgcgcgg
      481 ccagcgactt gccgcagccg tagacccccg tcaatccgac gcgcagcaca gtctccctcc
      541 taccggacca cggccagttt gccgagcctg accatgcctg aggcctggac gcggtagaga
      601 tagatcccgg ccggcaccag ccggccctga tcgtcgcgga catcccagac cagccccccg
      661 tcgccgttct cctcatacag gatgcgcagg gtgcggccct cggtggtcat gatgcggatt
      721 tccgcctcgg gggtgagatt ggcgaaggtg atcccctccg ggtcgatcca cagccggtag
      781 ggattgggaa aggtgtggac ctcgtcgagg tccgccgccg cgaagaggag ctggatggtg
      841 tccccgtggc ccggtttgat cgcggctccg ctcgcactct gcaggccgct caccctgagg
      901 gtgaggggct tgcccaccgc gccccaggcg acgctgccgg tgagatggag gcgcacgcag
      961 cgattctccg gcgcgaccgc ctgcgcccag gcgacatgca ggccggcgcc cggatcatag
     1021 ttggcgatct cctcgaggga agccttttgc atcggttcgc tgaattcaag ttcgaccacg
     1081 ccgctggcga tggcccgcac ggcggtcaga tagggggcgc cggcggcgaa agcgacctcg
     1141 aattcactct ggctgcggag ggtatcgacg gggaggccgt cgctgtcctc gaggccgcga
     1201 accccgacgc tgtaaaaacc ggattgggta aagggggcct caaggctgag cagcacctca
     1261 gcccccgagg ccaggggcag ggctgtggcc ggatggccga cctccggagc gatggtgtag
     1321 tggcggatat cctgcagcag ggcggaattc atcggccggg agaagcgcag gcgcacggag
     1381 cgtgcgccgg ccatcgcggc ggagagcagg gcgggctgct cacccggccg cgcagagacc
     1441 acctccgaac gcaggctcac ggccgggagg cgcgtggtgt ccctgctggc gaccgcatag
     1501 taatagaact cacccgcgcg gaccagggta tcggtgaaga ccggcagcag ggtctgctgc
     1561 aagagcttca gactgtcggc cgagaggccg cgaaagagca ggtaatcctg cgcccccggg
     1621 accggattcc acgacagcgc gactgtaccg gcattcaggg gcctggcagc cacccccacc
     1681 ggcatggccg gcccggtcag actctccgcc agcacacggg ggcggaccct ctccccgtcg
     1741 ctcgcccaga attccatcag gccgtcgcca tcgctgtcgg ccaccatcac cgcattggtc
     1801 cggatgggag cggcgtggaa ggtcagttca tagccccgct ccggggtaaa gctggcgacg
     1861 taaagatcgg gaaaagcggc gatgaagagt tcgtcgcggc cgtcgccgtc ggcatcgccg
     1921 caggagaccc cgctctcgaa atccctggcc gattcgtagc cgaagaagcg ccattccgcg
     1981 gcgggaaggt aggtgttgtt cccggtcgtg tcatagatgc ggtagcacca gtggcgggag
     2041 tcgtagctgt gctcggtatt gaggttggga tcagagtgcg agccggcggc gaattcgttt
     2101 tcgccgtcgc cgtcgaaatc cccggcggcg atgaaatcga tggcatcaag caggggcagc
     2161 cgatcctgcc aggttggggt gaaggcgctg ccgtcatatt cataaagata gatgtccccg
     2221 tcgctgtcgc cgaagaggag ttcaatgcgt ccgtcgccgt cgaaatcgcc ggtttcgcag
     2281 cgcggcaccc cattaaaatt gtcccctgcg ctgggattgg ccagcacggc catggtctcg
     2341 aactcgccgc ccccgctgct gcgccagacc tcgaatagat cagaggagct gtcaccggag
     2401 cggaccacac gcatgatcag ctcgctgcgg ccgtcgccat cgaggtccga cagccggctg
     2461 ccccagtact gcacccggct gtcgccctgc catttgcgca ccaggttcat gcggaagggt
     2521 tcggagccgc ccgcctcata gagccaggtg gaaaaaccgt agcccgccag cagttcagga
     2581 aggccgtcgc cgtcggtatc gccgctgctg cgcggaatgg cggactcggg cagggtataa
     2641 agctggcgg
//
LOCUS       DAOPWU010000433         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_40573_length_2640_cov_3.617408, whole genome shotgun sequence.
ACCESSION   DAOPWU010000433 DAOPWU010000000
VERSION     DAOPWU010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40573_length_2640_cov_3.617408"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKM23_14710"
     CDS             complement(<1..312)
                     /locus_tag="PKM23_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY92773.1"
                     /translation="MNPVSILIVDDEESVRYSLFNWFREEGYEVGIAKDAFEALAALD
                     KSPWDIMLLDVRMPGMSGIELQRRVREFHKEMIIIIMTAFASVDTAVQSLKEGAYDYV
                     VK"
     gene            complement(358..2001)
                     /locus_tag="PKM23_14715"
     CDS             complement(358..2001)
                     /locus_tag="PKM23_14715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY92774.1"
                     /translation="MRKLFQSLSFRIFILMMVIVTCGFGLFARRSVQTHSRELLESTT
                     VSAIRVSDIIQGAMRYGMLINRIEDIHQIINTLAADSGIAAIRLFDKNGTVISSSRRE
                     EVGSRLEISAKVCAECHAEGAAVSDLPLARRQRVLQDSAGLRILGVVNPIANEPDCYT
                     AGCHAHTPEERVLGVLDVQWSLTGVDAQLRSDRRQLILFGLLLILGCGLSAGWFIDRF
                     VRRRVQGLIRGTREIAAGHLDYRIEADEEDELGQLAAAFNHMSADLGAARRELTDWSL
                     HLEEKVDTKSRELAEAESRLARMERLASLGQLSATVAHEINNPLAGALNYSYLALRLL
                     QDDSLTQASRERLQEYLTIIKNEVGRSGDIINNMLLFARQSGGHFAEEHLHGLIASSL
                     KLVHHHMVLKGISCHEELNLEDDRLTCDAGQIRQALVALFVNAIEAMESGGVLTVCTS
                     AYPESAAIGIEVVDTGGGIPTDILPHIFDPFFSTKKEGKGVGLGLAVTFGIIQRHQGQ
                     IEVDSSVQEGTTFRILLPREPAVDKGERETSKAPATDSW"
     gene            complement(2202..>2640)
                     /locus_tag="PKM23_14720"
     CDS             complement(2202..>2640)
                     /locus_tag="PKM23_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92775.1"
                     /translation="LPPVREVILPRRGASLTTSQPTMWLITSDGTLGLTAPCDGVVRS
                     VNPGVLEELSQKAELPIQSFFMEIGVISSRRAVKGRLRGDKAAACLRAQYLEILQCLT
                     GGLGDESPEKESLHGKAPLTPGALSGLLGSKRYFNTVAPFYAL"
BASE COUNT          585 a          753 c          780 g          522 t
ORIGIN      
        1 cttgacgaca tagtcgtagg ccccctcctt aaggctctgc acagcagtgt ccactgaggc
       61 aaaggcggtc atgatgatga tgatcatctc tttgtggaac tcacgcactc gtcgctgcag
      121 ctcaatgcca ctcatccccg gcatgcgcac gtcgagcagc atgatgtccc atggcgattt
      181 gtccagcgct gccagggctt caaaggcatc cttggcgatg cctacctcat agccctcttc
      241 gcggaaccag ttgaacaggg aataccgtac ggattcctcg tcatcgacga tcaatatgga
      301 tacaggattc atatctcctc ggttgagtgt gcagcgatga aaataaaagc aagaaaatca
      361 ccagctatct gtagccggag ctttggaggt ttcacgctca cctttatcca cggcgggttc
      421 gcgtggcagg agaatgcgaa aagtcgtgcc ctcttgcacc gatgaatcga cttcgatctg
      481 gccctgatgg cgctgaatga tgccaaaagt caccgccagc cccagaccga ctcccttgcc
      541 ctcttttttg gtagagaaaa aaggatcaaa gatgtgcggc aggatatcgg tggggatgcc
      601 tcccccggta tccactacct cgatgccgat agccgcactc tccggatacg cactggtaca
      661 gacggtcaat acgccgccgc tctccatcgc ctcgatggcg ttgacgaaca gggcgaccaa
      721 agcctggcgg atctgcccgg catcacaggt caaacggtca tcctccagat tcagctcctc
      781 atggcatgag atccctttca gcaccatgtg atgatggacc agcttcaggc tggaggcgat
      841 cagaccgtgc aggtgttctt cagcgaaatg accgcccgac tgacgcgcaa agaggagcat
      901 gttgttgatg atatctcccg agcggccgac ctcgttttta atgatcgtca gatactcctg
      961 cagccgctcc cgggacgcct gtgtaaggga gtcgtcctgc agcagccgca aggcgaggta
     1021 ggagtaattc agggcgccgg cgaggggatt gttgatttca tgggcaacag tggcggagag
     1081 ttggcccagt gaggccagcc tctccatccg tgccaggcgg gattcggctt cagcgagctc
     1141 gcggcttttt gtgtcgacct tttcctcgag gtgcagcgac caatcggtca gctctcttct
     1201 ggccgccccc agatccgcgc tcatatggtt gaaggcggcg gcgagctgtc ccagttcatc
     1261 ttcttcatct gcctcgatgc gatagtcaag atgccccgca gcgatttcac gcgtgcccct
     1321 gatcaacccc tggacgcggc ggcgcacaaa acggtcgatg aaccagccgg cggagagccc
     1381 gcaccccagg atgagcagca gcccgaacag gatcaactgc cggcggtcag atcggagttg
     1441 ggcatcgact cctgtcaggg accactgcac atccagcacc cccagcaccc gttcctcagg
     1501 agtgtgggca tggcagcccg cggtgtagca gtcgggttcg ttggcgatgg gattgacaac
     1561 tcccaggata cgaaggcctg cactgtcctg cagtacacgc tggcgcctgg ccaatggcaa
     1621 atcggagacg gccgcaccct cagcatgaca ttcggcgcac accttcgcag atatctccag
     1681 gcggctgccg acctcctcac gccgcgaaga ggagataacc gtaccgttct tgtcgaagag
     1741 gcggatggct gcaattccgg aatccgcagc gagggtgttg atgatctgat gaatatcctc
     1801 gatgcggttg atcagcatac cgtaccgcat cgcgccctga atgatatcgc taaccctgat
     1861 ggcgctgacc gtggtgctct cgaggagctc ccggctgtgg gtttgcaccg aacggcgcgc
     1921 gaagagcccg aagccgcagg tgacaatgac catcatcagg atgaatatgc ggaaactgag
     1981 cgactggaaa agttttctca tttggcagca gggatgggat attccgacct gggcaattta
     2041 attaaattaa agatttattt gcagaggcgc aacattgatt gtaaaggaat tcgccggaat
     2101 gcagtaaaat gcaacaatcc atgttgaatt aatagacaaa cactggcagg gtggaaaaga
     2161 gtaggcaggc agcgggggtg gaatcaggtc cgggagaggc gtcagagcgc ataaaaggga
     2221 gctacagtgt tgaaatagcg cttgctgccg agaagtccgg acagggcacc gggggtgagg
     2281 ggagccttgc cgtggagcga ctccttttca ggggattcgt cgccgagacc gccagtcaga
     2341 cactgcagga tctcaaggta ctgcgctcgc agacaggccg cagccttgtc ccccctcaac
     2401 ctccccttga cagcgcggcg gctggagatc accccgatct ccatgaaaaa ggactggatg
     2461 ggcagctctg ctttctgcga cagctcctcg agcacgccgg gattgacaga gcgcaccacg
     2521 ccgtcacagg gtgcagtcag tccgagggtg ccatcactgg tgatgagcca catggtcggc
     2581 tgggaggtgg tgagggaggc gccccggcgg ggcagaatga cctcccgcac gggcggaaga
//
LOCUS       DAOPWU010000434         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_40591_length_2639_cov_4.184598, whole genome shotgun sequence.
ACCESSION   DAOPWU010000434 DAOPWU010000000
VERSION     DAOPWU010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40591_length_2639_cov_4.184598"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1074)
                     /locus_tag="PKM23_14725"
     CDS             complement(226..1074)
                     /locus_tag="PKM23_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92776.1"
                     /translation="MMRIALGILAGLVVLIVLALIFGQWSFNRMVRQELTAFLPARLP
                     EGEMVTPGRIAHLPEPVQRWLVRSGVVGREEIHLVHLRQRGEMLNKPGGPWLPVDSEQ
                     YFRTAEPGFIWIADVRMMPLLHLAGRDMYQDGRGRMVIKLASLLPVVNASGAETDQGT
                     MLRWLAETVWFPSAALSPHITWEAVDSLSARATMSYGGVTAAGLFTFTPEGDFSSFIA
                     DRYYYRKEGSTLEPWQVKVEEGGYGELGGIRMPLRCSVTWRLKEGNFTWYKLVIESAE
                     YDQAVR"
     gene            complement(1071..2006)
                     /locus_tag="PKM23_14730"
     CDS             complement(1071..2006)
                     /locus_tag="PKM23_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003396938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNY92777.1"
                     /translation="MEKKSKIYIAGHKGMVGSALVRNLEGKGHRNLLVRSLEELDLLD
                     QAAVFNFMAAEKPEYVFIAAAKVGGILANNTFRAQFIYENLQIQANLIHAAHINRVKK
                     LLFLGSSCIYPKLAPQPIREEHLLTGLLEPTNEPYAIAKIAGIKMCEAYRAQYGSNFF
                     AVMPTNLYGPNDNFDLQTSHVLPALIRRFHEAKAGSQPFVTIWGTGSPRREFLHVDDL
                     ADACVFLMNKYDRDEILNIGVGEDLTIRELAELIRDVVGYSGEIRYDTGKPDGTPRKL
                     LDVSRLRATGWRPKIGLKEGIQRTYAWYLDNKAEK"
     gene            complement(2018..>2639)
                     /locus_tag="PKM23_14735"
     CDS             complement(2018..>2639)
                     /locus_tag="PKM23_14735"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNY92778.1"
                     /translation="PQSETTPFRPRSPYAAAKLYAYWMTVNYREAYNLFACNGILFNH
                     ESPRRGETFVTRKITRAVARIAHGLEETVFLGNLAAQRDWGYAPEYVDGMWRMLQQPE
                     PGDYVLATGVMHTVREFAALAFREIGVELEWQGEGREEKGIDPKTGRVRVAVDPNYFR
                     PAEVDLLVGDASKARTVLGWEPKTTLPELVHLMVQADLEKVKRRGY"
BASE COUNT          472 a          794 c          892 g          481 t
ORIGIN      
        1 taatttaata caagacagct ccttccagcg gtttcggagt gcaggggaga aagtgccgga
       61 gcgtgggtac ctgtggcagg tcggatgggc gcgagagctc aatgtgaact caggccgggt
      121 cggcatgaga ggggaagtgg ggtcggtacg agggctcagg cgcgagagga gccgggtcgg
      181 cgcgagagct aaagtgcgac caaaggccgg gtgggcggga gaatgctaac gcaccgcctg
      241 gtcgtactcc gccgactcga tgaccagctt gtaccaggtg aaattcccct ccttgagccg
      301 ccaggtcacc gagcagcgca gcggcatgcg gatgccgccc agctcgccat agcccccctc
      361 ctccaccttg acctgccagg gctcgagggt cgatccctcc ttgcggtagt aataacggtc
      421 ggcgatgaag ctgctgaaat ccccctccgg ggtgaaggtg aacagccctg cagcggtgac
      481 gccgccataa ctcatggtcg cccgggccga gagggaatcc actgcctccc aggtgatatg
      541 cgggctgagt gctgccgagg gaaaccagac ggtctcggcc agccagcgca gcatggtgcc
      601 ctggtcggtc tcggcgccgc tggcgttgac cacaggcagc agcgaggcga gcttgatgac
      661 catgcggccg cggccgtcct ggtacatgtc gcggccggct aggtgcagaa ggggcatcat
      721 tctgacatcg gcgatccaga tgaatccggg ctcggcggtg cgaaaatact gctccgagtc
      781 caccggcagc cagggcccgc cgggcttgtt gagcatctca ccgcgctggc gcaaatgcac
      841 caggtggatc tcctcgcggc cgacaactcc gctgcgcacc agccagcgct gcaccggctc
      901 cggcagatgg gcgatccggc cgggggtgac catctcccct tccggcaggc gcgcgggcag
      961 aaaggcggtc agctcctgac gcaccatgcg gttgaaactc cactgcccga aaatcagggc
     1021 cagaacgatc agaacaacca atcccgccag aatgccgagg gcaatgcgca tcatttctcc
     1081 gctttgttat ccagatacca ggcgtaggtc cgctgaatcc cctctttcag cccgatcttc
     1141 gggcgccaac cagtcgcgcg cagccggctg acatccagca gtttgcgcgg ggtgccgtcc
     1201 ggcttgccgg tgtcgtagcg gatttcaccg ctgtagccga cgacgtcccg gatcagctct
     1261 gcgagctcgc ggatggtgag atcctccccg acgccgatgt tgaggatttc gtcgcggtca
     1321 tatttattca tgagaaaaac gcaggcatcg gcgagatcgt cgacatggag gaactcgcgg
     1381 cggggcgagc cggtgcccca tatggtcaca aaaggttggc tgccggcctt cgcctcatga
     1441 aaacggcgga tgagggcggg caggacgtgc gaggtctgca ggtcgaaatt gtcgttgggg
     1501 ccgtaaagat tggtcggcat gaccgcgaag aaattgctgc catactgcgc gcggtaggcc
     1561 tcacacatct tgatgccggc gatcttggcg atggcgtagg gctcgttggt cggctcgagc
     1621 agtccggtga gaagatgctc ctcgcggatg ggctgcggcg cgagcttggg atagatgcag
     1681 gagctgccga ggaagaggag ctttttaacc ctgttgatat gggcggcgtg gatgaggttg
     1741 gcctggatct gcagattctc gtagatgaac tgggcgcgga aggtgttatt ggcaaggatc
     1801 cctcccacct tggcggcggc gataaaaaca tactcgggct tctccgcagc cataaagttg
     1861 aaaaccgccg cctggtcgag gaggtcgagt tcctcgaggc tgcgcacgag gagattgcga
     1921 tggcccttgc cctcgaggtt gcgcaccagg gccgatccga ccatgccctt gtggccggcg
     1981 atatagattt tactcttctt ctccattgct ctcccgttca gtatcccctg cgtttcactt
     2041 tctccaggtc ggcctggacc atgagatgaa ccagctcggg cagggtcgtc ttcggctccc
     2101 agcccagcac ggtgcgcgcc ttgctggcgt cgccgacgag gaggtcaacc tcggccgggc
     2161 ggaaatagtt gggatcgacc gccacccgca cccggccggt tttggggtcg atgcccttct
     2221 cctcgcgccc ctcgccctgc cattccagct ccaccccgat ctcgcggaag gcgagcgcgg
     2281 cgaattcgcg cacggtgtgc atcaccccgg tggcgaggac gtaatccccg ggctcgggct
     2341 gctgcagcat ccgccacatc ccatcgacgt actcgggggc gtagccccag tcgcgctgcg
     2401 cggcgaggtt gccgagaaag acggtctcct ccaggccatg ggcgatgcgg gcgaccgcgc
     2461 gggtgatctt gcgggtgacg aaggtctcgc cgcggcgggg cgactcgtgg ttgaagagga
     2521 tgccgttgca ggcgaagagg ttgtaggcct cgcggtagtt caccgtcatc cagtaggcgt
     2581 agagcttggc ggcggcgtag gggctcctgg ggcggaaggg ggtcgtctcg ctctgcggg
//
LOCUS       DAOPWU010000435         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_40672_length_2635_cov_5.200000, whole genome shotgun sequence.
ACCESSION   DAOPWU010000435 DAOPWU010000000
VERSION     DAOPWU010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40672_length_2635_cov_5.200000"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKM23_14740"
     CDS             complement(<1..509)
                     /locus_tag="PKM23_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNY92779.1"
                     /translation="MAIDLQKMRNIGISAHIDSGKTTLTERILYYTQKIHAIHEVRGK
                     DGVGATMDSMDLERERGITISSASTNVTWKKSMINIIDTPGHVDFTIEVERSLRVLDG
                     AVLVLCAVGGVQSQTLTVDRQMTRYKVPRLAFINKCDRMGADPERIVEQLRDKLGHNA
                     VLMQLPIGL"
     gene            complement(680..>2635)
                     /locus_tag="PKM23_14745"
     CDS             complement(680..>2635)
                     /locus_tag="PKM23_14745"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNY92780.1"
                     /translation="LVDSARVFEFNDYAWSPNNKWIVYTRNEEEMMPRLYLYSVATAT
                     TAAISDGWFDCANPVFSSDDRYLFFVSDRDFNPLYSQIEWNHIYRDMSRIYMLTLAKD
                     TPSPFQPKSDEVQIGGRTTTGSASKSDKKEAKKENSFSIATSRIDLEGLAQRIIGLPV
                     KPANYGSLASVEDKLYYIRRSSRDEKPILLFYDLKELKETELGQIDGYEISADLKKMI
                     VRQGSTYAIIDLPSAKIEIKEKLNLTGMEVNLDRKAEWQQIYNESWRQMRDFFYAPNM
                     HGVDWRAVRDKYAPLLPYVEHRADLSYLIGEMIGELGAGHTYVGGGDLPKPRRIKLGL
                     LGARLSKDPRSGYFRIDRILKGENWQAKTRSPLTEIGVDAREGDYILAINGQSTAEMP
                     NLYAALAGKAGRQVQLTLNSTPNTAGSREVTVVPIDNEAELYSYSWVQANIARVNEAT
                     GGQVGYIHIPDMGVNGLNEFVKYYYPQLQKKALIIDDRGNGGGNVSPMIIERLRREIA
                     MFTFARNTVASVDPGGMQWGPKICLIDEFSASDGDLFPYRFRQMKLGKLVGKRTWGGV
                     VGIRGSLPFLDGGSLNKPEFSRFGLDGREWIIEGTGVEPDVWVDNDPALEFNGTDQQL
                     DKAIELILEELKNWQNTVPPVPPYPVR"
BASE COUNT          471 a          696 c          874 g          594 t
ORIGIN      
        1 tcgaggccga tcggaagctg catcagtact gcgttatggc cgagcttgtc gcgcagctgt
       61 tcgacgatgc gttccggatc agcgcccatg cgatcgcact tgttgatgaa ggcgaggcgg
      121 gggactttgt agcgggtcat ctgccggtcg acggtgaggg tttgggattg cacgccgccc
      181 acggcgcaga ggacgagcac agcgccgtcg aggacgcgca gagagcgttc cacctcgatg
      241 gtgaaatcga cgtgtcccgg ggtatcgatg atattgatca tcgatttctt ccaggtcaca
      301 ttggtggagg cggaggagat ggtgatgccg cgttcgcgct cgagatccat cgagtccatg
      361 gtggcgccta cgccgtcctt gccgcggact tcgtggatgg cgtggatttt ttgggtgtag
      421 tagaggatcc gttcggtcag cgttgtcttg cccgagtcga tgtgggcgct gatgccgata
      481 ttgcgcattt tttgcagatc gatagccata ttcaactctg agtttatgat gaaattcgcg
      541 aaaaacaaag agttaatttt acgaattttt catcatattc ccaacaaaaa aatgaccgat
      601 ttccgccccg gcctgagaat cgcatgctgt ccgaaaaggg cctccttgcg gctgccccgc
      661 cgcaaggagg cggaaccgct cagcgcactg gatagggggg gacaggcgga acggtgttct
      721 gccagttttt cagctcctcg aggatcagtt cgatcgcctt gtcgagctgc tgatcggtgc
      781 cgttgaattc gagggcggga tcgttgtcga cccagacatc cggctccacg ccggtgcctt
      841 cgatgatcca ctcccggccg tcgaggccga agcgggagaa ttcaggcttg ttgagcgagc
      901 cgccgtcgag gaagggcagg gagccgcgga taccaaccac gccgccccag gtgcgcttgc
      961 cgacgagctt gcccagcttc atctgcctga agcggtaggg gaagaggtca ccgtcggagg
     1021 cggagaactc gtcgatcagg cagatcttcg ggccccactg catgccgccg ggatcgaccg
     1081 aggccacagt gttgcgggcg aaggtgaaca tggcgatctc gcggcggagc cgttcgatga
     1141 tcatgggcga gacgttgccg ccgccgttgc cacgatcgtc gatgatcagg gcttttttct
     1201 gcagctgggg gtagtagtat tttacgaact cattgaggcc gtttacgccc atgtcgggga
     1261 tatggatata gccgacctgg ccgccggtcg cctcgtttac tcgggcgatg ttggcctgga
     1321 cccaggagta gctatagagc tccgcctcat tgtcgatcgg tacgactgtg acctcgcggc
     1381 tgccggcggt attgggggtg ctgttgaggg tcagctgcac ctgcctgccg gccttgccgg
     1441 cgagggcggc atagaggttg ggcatctccg cggtggattg cccgttgatg gcgaggatat
     1501 agtcgccttc gcgagcgtcg accccgatct cggtgagggg ggagcgggtt ttggcctgcc
     1561 agttctcccc tttgaggatc ctgtcgatgc ggaagtagcc gctgcgggga tccttgctga
     1621 ggcgggcgcc gagcagcccc aatttgatgc gccggggttt gggcaggtcg ccgccgccga
     1681 cgtaggtgtg gccggcgccg agttccccga tcatctcgcc gatgaggtag gagaggtccg
     1741 cgcggtgctc cacatagggg agcagggggg catatttatc ccggacggcg cgccagtcca
     1801 ccccgtgcat gttgggggcg tagaaaaagt cgcgcatctg ccgccagctc tcgttgtaga
     1861 tctgctgcca ttccgccttg cggtccagat tcacctccat gccggtgagg ttgagctttt
     1921 ccttgatctc gatcttggcg gagggcagat cgatgatggc gtaggtggag ccctgccgga
     1981 cgatcatctt tttcagatcg gcggagatct cataaccgtc gatctgaccc agctcggtct
     2041 ccttgagctc cttcaagtca tagaaaagca ggatgggctt ctcgtccctg ctgctgcgac
     2101 gtatatagta gagtttgtcc tcgacggagg cgaggctgcc atagttggcg ggcttgactg
     2161 gcaggccgat gatgcgttga gccagtccct caagatcgat ccgggaggtg gcgatggaga
     2221 atgaattctc cttcttggct tcttttttat cgcttttact ggcggaccca gtcgtggtcc
     2281 ggccgccgat ctgcacctcg tcgcttttcg gctggaacgg ggagggggta tctttggcca
     2341 gagtcagcat atagatgcgc gacatgtcgc gatagatgtg gttccattcg atttgactgt
     2401 agaggggatt aaaatcgcga tcggagacga aaaagaggta gcggtcatcg ctgctgaaga
     2461 ccgggttggc gcagtcgaac cagccgtcgc tgatagcggc agtggttgcg gtggccactg
     2521 agtagaggta gaggcgcggc atcatctcct cttcgttccg ggtgtagacg atccatttgt
     2581 tgttgggcga ccaggcatag tcgttgaatt caaagacccg ggcgctgtca acgag
//
LOCUS       DAOPWU010000436         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_40970_length_2621_cov_3.545986, whole genome shotgun sequence.
ACCESSION   DAOPWU010000436 DAOPWU010000000
VERSION     DAOPWU010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40970_length_2621_cov_3.545986"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /gene="nrfD"
                     /locus_tag="PKM23_14750"
     CDS             <1..330
                     /gene="nrfD"
                     /locus_tag="PKM23_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNY92781.1"
                     /translation="EMLARADNLFLAIELFILALFFIGLLSSSEVQVKSAMLLLTGPY
                     AAVFWVFVILTGIIIPLLIQNLSVSHHIKHTPIAPIMVIIGGFILRFIFVQAGQYSHY
                     LNAHFIK"
     gene            355..894
                     /locus_tag="PKM23_14755"
     CDS             355..894
                     /locus_tag="PKM23_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNY92782.1"
                     /translation="MTVHSSDDFAPKPYWNPYLAGIGLGLVLLASFVIMGRGLGASGA
                     FSSAVAWGVQRVSPEHANSNPFYAEYLGDGTTNPLKDWLVFEVIGVFIGGFISAKLAH
                     RVKGKLEKGPRISNRGRLLYAFLGGGMMAIGAKLARGCTSGQALTGGALLNLGSWAFM
                     ISVFIGGYALAYIMRRQWL"
     gene            891..1490
                     /locus_tag="PKM23_14760"
     CDS             891..1490
                     /locus_tag="PKM23_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNY92783.1"
                     /translation="MSAPFFKYGLFGSEASLIVAVFIGIGFGFFLERAGFGSARKLAA
                     QFYFTDMTVLKVMFTAIVTAMVGVYYLSVFGVLDLSLVYLTPTYLAPQIAGGLILGFG
                     FVVGGYCPGTACVSASTGRYDGWIYVGGLFAGILFFGEVFHWVEKFYASTPMGQVTLP
                     QLLHMPYGLLVFLVCVMAVGAFAAAEWGEKKMAAKKEGK"
     gene            1487..2152
                     /locus_tag="PKM23_14765"
     CDS             1487..2152
                     /locus_tag="PKM23_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNY92784.1"
                     /translation="MTMKILNSLKRQSLNTKLALALLLLGFLAIFAGDPYGNAKTEID
                     AKELALIVDKQVDHIAAEELADWIIQNRADYRLVDLRTEEEFTTYHIPTAENIPLAGL
                     IDGGLQRNEKIVLYSEGGIHSAQAWFLLKAKGYKAVYMLFGGLEEWKDRVLFPAVPEN
                     PTPEQLAAFAKIKEVSKYFGGTPQSGAGSVSAQPKTEMPKLSLPAGGAAPKPQAKKKK
                     EGC"
     gene            2178..2453
                     /locus_tag="PKM23_14770"
     CDS             2178..2453
                     /locus_tag="PKM23_14770"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HNY92785.1"
                     /translation="MPVLYKTDIASDADLQVCFISIRAEADLVVFESSSAWEATESHI
                     WCYTDIRSEANRVVYVTDSPFEADLLVFRTDIQSDAGWQNSGKSGLL"
     gene            2494..>2621
                     /locus_tag="PKM23_14775"
     CDS             2494..>2621
                     /locus_tag="PKM23_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNY92786.1"
                     /translation="MKILSILGTRPEAIKMAPVIKELEKHPQEVESLICVTGQHRQ"
BASE COUNT          548 a          733 c          720 g          620 t
ORIGIN      
        1 gagatgctgg cacgggcgga caatctcttc ctggccatcg aactcttcat cctggccctt
       61 ttcttcatcg gcctgctcag ttcgtcagaa gtgcaggtca aatccgccat gctgctgctc
      121 acgggaccat atgctgcggt gttttgggta ttcgtcattc ttacgggaat tatcatcccc
      181 ctgctgatcc agaacctttc ggtcagccat cacatcaaac atacaccgat tgcaccaata
      241 atggtcatca tcggcggttt catcctgcgg tttattttcg tgcaagcagg acagtacagc
      301 cactatctca atgcccattt tatcaaataa tccacttcaa cggaggaatg atcgatgaca
      361 gtgcattcat ctgatgactt tgctcccaag ccctactgga atccctattt ggcgggaata
      421 gggctggggc tggtgctgct ggcttcattc gtaataatgg gaaggggcct cggggcttcg
      481 ggggctttct cctctgcagt agcctgggga gtgcaaaggg tctcgccaga gcatgccaac
      541 tccaatccct tttacgccga atacctggga gatggcacca ccaatcctct caaggactgg
      601 ctggttttcg aggtgatcgg ggttttcatc ggcggcttta tctcggccaa actggcccat
      661 cgcgtcaagg ggaaacttga aaaggggccg cgcatcagca accgcggccg gctgctctat
      721 gcatttttag gcgggggaat gatggcgatc ggcgccaaac tggcgcgcgg ctgcaccagc
      781 ggccaggccc tcaccggcgg cgctttgctc aatctgggca gctgggcttt tatgatctcc
      841 gtcttcatcg gcggctacgc ccttgcttac atcatgagga gacaatggct atgagcgcac
      901 cctttttcaa atacgggctg ttcggcagcg aggcgagtct gatagtcgcc gtatttattg
      961 gaatcggctt tggctttttt ctcgagcgcg ccggttttgg cagcgcgcgc aaactggcgg
     1021 cccagttcta tttcaccgat atgaccgtgc ttaaggtgat gttcaccgct attgtcaccg
     1081 ccatggtcgg tgtctattat ctctctgtct tcggtgtgct ggatctctcc ctggtctatc
     1141 tcaccccgac ctatctggcg cctcagatcg caggggggct gatcctcggc tttggcttcg
     1201 tcgtcggcgg atactgccca ggcactgcat gtgtctcagc ctcgaccggc cgctatgacg
     1261 gctggatcta tgtcggcggg cttttcgcag gcatcctctt ttttggcgag gtcttccatt
     1321 gggttgagaa gttttacgcc tcgacaccca tgggccaggt aaccctgccg caattgctcc
     1381 atatgcccta cgggcttttg gttttcctgg tttgcgtcat ggccgtgggt gcttttgccg
     1441 ctgccgaatg gggggagaaa aagatggccg cgaagaagga gggcaaatga cgatgaaaat
     1501 ccttaattcc cttaaaagac aaagtctgaa taccaaactt gcgctagcac ttcttctcct
     1561 cggctttctc gccattttcg ccggggatcc ctatggcaat gccaaaacag aaatcgatgc
     1621 caaagagctg gcactgatcg tcgacaaaca ggtcgatcat atcgcggccg aagaactggc
     1681 cgactggatc atccaaaata gggcggacta tcgcctggtc gatctgcgca cggaggagga
     1741 gttcacgacg taccatatcc cgaccgcgga gaatatcccc cttgccggcc tcatcgacgg
     1801 tggactgcag cgtaatgaaa agatcgtgct ctattcggag ggcggcatcc attccgccca
     1861 ggcctggttc ttgttgaagg cgaagggcta caaagcggtt tacatgctct tcggcggatt
     1921 ggaggaatgg aaggataggg tgcttttccc ggcggtgccg gagaatccaa cccctgaaca
     1981 gctggccgct tttgctaaaa tcaaagaagt aagcaagtat ttcggcggca ctccccagag
     2041 cggcgcagga tctgtgagcg cccagccgaa gacagagatg cccaagctga gcctgcccgc
     2101 tggcggcgct gctcccaaac cacaggcaaa aaagaaaaaa gagggctgct gatttaattc
     2161 aatcatggag aaaagtcatg cccgttctct acaaaaccga cattgcctcg gatgccgacc
     2221 ttcaagtctg tttcatcagc atccgtgccg aggctgatct ggtcgtcttc gaatcctcca
     2281 gcgcctggga ggccacagag tctcacatct ggtgctacac ggatattcgc agcgaagcga
     2341 accgggtggt ttacgtcacc gattctcctt tcgaggccga tctgctggtc ttccggaccg
     2401 atatccagtc cgatgccggc tggcagaaca gcggcaagtc cggcctcctg tgatgccgca
     2461 gcttgtgctc atcactattt gtcaggtaac acagtgaaaa tcctctcgat cctcggtacg
     2521 cggccggagg cgatcaagat ggccccggtg atcaaggaac tggaaaaaca tccccaggaa
     2581 gtggagagcc tgatctgcgt caccggccag caccggcaga t
//
LOCUS       DAOPWU010000437         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_41273_length_2608_cov_3.372895, whole genome shotgun sequence.
ACCESSION   DAOPWU010000437 DAOPWU010000000
VERSION     DAOPWU010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41273_length_2608_cov_3.372895"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKM23_14780"
     CDS             complement(<1..778)
                     /locus_tag="PKM23_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY92787.1"
                     /translation="MISSPRKAKSTSGFSRNLLLTGLTSFLTDTSVKMVYSVMPMFLL
                     SIGASKTSLSLIEGIAESTAALVKTLSGFWSDRIGRNKPFMLVGYGITALIIPLYALV
                     VSPVQVLLLRFIERFGKGVRTAPRDSLIAGSVINGETGKGFGLQKAMDNSGAIVGPLA
                     AFALLYLFPADYRRLFFLAGIPSLLGILVILFGIREAKKGSDQLFKKFRFRDFPGRFY
                     LFLAIIFIFTLGNSTDALLMVKANEVGVKVAFIPLVYLVTS"
     gene            complement(876..1577)
                     /locus_tag="PKM23_14785"
     CDS             complement(876..1577)
                     /locus_tag="PKM23_14785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY92788.1"
                     /translation="MEIPDNTIEAFARNFHREARLYGFSDADCLRFVNAILDLIMKND
                     VANTHKFSTLHWSREGEQRYARLPITMEQLCIREYDHIIDPPFFERWLEDEYGRYFLL
                     SRLSLHRTDFFDLVNDPTNIIGVITLPEGLPIGAVAFLKHDILSKKAELRKLIGNPEY
                     RGKGYAKTATRLWIEYGLFGLGLKKIYLNTINTNIRNIRLNQELGFTIEGILHNEVMI
                     DGVYKDVLRMSLWLE"
     gene            1741..2496
                     /locus_tag="PKM23_14790"
     CDS             1741..2496
                     /locus_tag="PKM23_14790"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNY92789.1"
                     /translation="MQNEEQSGQGVRVGLVRAFSKKGLGSRTQAREWILAGRVKVNGR
                     VEKFIYAWVDLEKDQITLDDQPIGRVEEKLYYLFHKPAGFLTARVDQESRATVYDLLP
                     GFATWVFPAGRLDMESEGALFLTNDGPMASWLVSPGSGIEKCYRVRINRPLQEGDRKT
                     LEEGILIRNYRTLPARLRILPDVKPGHWAEIIIHEGKNRQVRRMFAACGYEVVRLIRT
                     RIGPLELGDLPAGAWRPLTPAELEALRTLQAGR"
BASE COUNT          549 a          678 c          843 g          538 t
ORIGIN      
        1 tgctggtgac caggtagacg agcgggatga aggcgacttt cacccccacc tcattggcct
       61 tgaccatgag cagggcgtcg gtggaattgc cgagggtgaa gatgaagatt atagccagga
      121 agaggtaaaa ccgcccgggg aaatcccgga agcggaactt tttaaagagc tgatcgctgc
      181 cctttttcgc ctcgcggatg ccgaaaagga tcaccaggat gcccagcagg gaggggatcc
      241 cggccaggaa gaagaggcgc ctgtaatcgg ccgggaacag gtagaggagt gcaaaggcgg
      301 ccagggggcc gacgatggcg ccgctgttat ccatcgcctt ttgcaggccg aatcccttgc
      361 cggtctcgcc gttgatgacc gagccggcaa tgaggctgtc gcgcggtgca gtgcggaccc
      421 ccttgccgaa cctctcgatg aagcgcagca gcaggacctg cacgggggag acgaccaggg
      481 cgtagagagg gatgatgagg gcagtgatgc cgtagcctac cagcatgaag ggcttgtttc
      541 tgccgatgcg gtcgctccag aaaccggaga gggtcttgac gagggcggcg gtgctctccg
      601 cgatgccctc gatcagcgag aggctggtct tggaggcgcc gatggagagc agaaacatcg
      661 gcatgaccga atagaccatc ttcaccgagg tgtcggtgag aaagctggtc agccctgtta
      721 agagcagatt gcggctgaat ccggaggtgg atttggcttt tcgaggcgat gagatcattc
      781 tgtcggagac tccattatca tcggcggttt tatcagccag cgcacgggcg ccggtccccc
      841 attttgcacg cgctgcggct gtacggaata tttccttatt ccagccacag gctcatcctg
      901 agcacatcct tgtagacccc gtcgatcatg acctcgttgt ggaggatgcc ctcgatggtg
      961 aagccgagct cctgattgag gcggatgttg cggatattgg tattgatggt gttgaggtag
     1021 atttttttca gaccgaggcc gaagaggccg tattcgatcc agaggcgggt ggcggttttg
     1081 gcgtagccct tgccgcgata ttcgggattg ccgatgagct tgcggagttc agcctttttg
     1141 gagaggatat catgcttgag aaaggcgacg gctccgatcg ggagcccctc gggcagggtg
     1201 ataaccccga tgatattggt ggggtcattg accagatcga aaaaatcggt ccggtgcagg
     1261 ctcagccggg agaggaggaa atagcggccg tattcgtcct cgagccatct ttcgaaaaag
     1321 ggggggtcga tgatatggtc atattcacgg atgcagagct gttccatggt gatgggaagg
     1381 cgggcgtagc gctgctcacc ctcccgtgac cagtgcaggg tgctgaattt gtgggtgttg
     1441 gccacgtcat tcttcatgat cagatcgagg atggcgttga cgaagcgcag gcagtccgca
     1501 tcgctgaatc cgtacaggcg tgcttcacgg tggaaattgc gcgcaaaggc ttcgatggtg
     1561 ttgtcgggga tttccatacg gcgaaggagg ctcctataag atccgtaact gccatatgaa
     1621 tttatgaagc ctgaacgaat taaaaaagct atatttccag ctgaaccatt ttatccttgc
     1681 cccatctctg caaatcacct atattttacc agccttgtga actgatgaat ggaccagatc
     1741 atgcagaatg aagaacagag cggccagggg gtgagggtag gactcgtccg ggccttcagc
     1801 aaaaaggggc tgggttcccg cacccaggcg cgggaatgga tcctcgcggg ccgggtcaag
     1861 gtcaacggcc gggtcgaaaa attcatctac gcctgggtcg acctggaaaa ggaccagatc
     1921 accctggacg atcagcccat cggccgggtg gaggaaaagc tctattatct ttttcacaaa
     1981 ccggccggat ttctcactgc ccgcgtcgat caggagagcc gggcgacggt ctatgacctc
     2041 ctgcccggtt tcgccacctg ggtctttccc gccggccggc tcgacatgga gagcgagggg
     2101 gccctcttcc tgaccaacga cggcccgatg gcgagctggc tggtctcccc cggcagcggg
     2161 atcgaaaagt gctaccgggt gcgtatcaac cgccccttgc aggaaggcga ccggaagacc
     2221 ctggaggagg gcattctgat caggaattac cgcaccctgc cggcgcgcct gcgcattctg
     2281 cccgatgtaa aaccgggcca ttgggcggag atcatcattc atgagggcaa aaaccggcag
     2341 gtgcggagga tgttcgctgc ctgcggttat gaggtggtgc gtctcattcg cacccgcatc
     2401 ggcccgcttg agctcggcga tctgcctgcc ggcgcctggc gccccctcac tcctgccgaa
     2461 ctggaggcct tgcgcaccct gcaggccggg agataaaaaa agccggaggc tcgcaagcct
     2521 ccggcttttt gctgcatggt gcggacgggg ggttattttc gattaggaaa attttccggt
     2581 tggttgctga cttggaaatg aagagatg
//
LOCUS       DAOPWU010000438         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_41984_length_2580_cov_3.541386, whole genome shotgun sequence.
ACCESSION   DAOPWU010000438 DAOPWU010000000
VERSION     DAOPWU010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41984_length_2580_cov_3.541386"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1313)
                     /locus_tag="PKM23_14795"
     CDS             complement(54..1313)
                     /locus_tag="PKM23_14795"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HNY92790.1"
                     /translation="MKVHNTVCSLLLALIAGVAVLGCSESGGPVEPPPSTIAYTPPKD
                     STFVVINSNRVVEGYLTPSKWYSYSWEDIDYTSVTHIVYSFLRPTSSSDPALTYGDLE
                     YEKAAYSLYPGLADKTHLGYGDRVIARAHAANVKVLIGVAGRQGQNARDLAVIFAIEK
                     YRKQFISNLVKLCEQRNYDGVNLDYEYPASSEDGLGITDFAQELRVAFVKSAKLSKRE
                     MFITMAVPTGDWAGKYFDFARLAKCCNWFSPMTYEFATSWTTRFGFNSPIYTDAGTGT
                     STAIVTTVDYLRNTRRVNPLQIVIGVPFFGWVFSNYTGLGNYKTNGNGADKSYTGLYN
                     QYIQGMPDNGFVYQWNDVCKQPYLVNTASRQMITYDDDKAVIEKANYIKANGLKGAMI
                     WELGRGFISGAVDPNPLLTALGNHLLH"
     gene            complement(1469..2389)
                     /locus_tag="PKM23_14800"
     CDS             complement(1469..2389)
                     /locus_tag="PKM23_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNY92791.1"
                     /translation="MKTRYPLHGLTAAAHTPFTADGALNLAAVEKQCEHLLRNNVTQV
                     FIGGSTGESHSVGTDERRQLAERWAEVIKGTPMRLIVHVGSNCLRDARALAEQAEELG
                     VLAIAALAPGYFRPNTLDRLIACCAGIAAGAPSTPFYFYDIPALTNVNFAMDEFLERG
                     EAAIPTLAGIKFTNSDLMAYQLTLRAGGGGFDVPWGVDEFMLGALALGAKGAVGSSFN
                     FAAPVYQRLIRAFEAGDLAAAREEQWKSVQLIKALASVGYLGAAKALMGWLGVEVGPA
                     RLPNGNPSAEQLKALRAGLERMGFFEWIRG"
     gene            complement(2386..>2580)
                     /locus_tag="PKM23_14805"
     CDS             complement(2386..>2580)
                     /locus_tag="PKM23_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92792.1"
                     /translation="AHFPDPEYSEWYGYLHRDGRVSMPLKGNHWKGFFHLPRMLWYCW
                     KLLEEIPTVPEEDITHRERR"
BASE COUNT          539 a          755 c          746 g          540 t
ORIGIN      
        1 atgcttcatg ctccgttatt tgatttaaat cctgacttgc tcctccccgg gcattagtgc
       61 agcaggtggt ttcccaacgc ggtcagtaag ggattgggat ccacggcgcc gctgataaag
      121 cctctgccca gttcccagat catggctccc ttgagcccgt tggctttgat atagttggcc
      181 ttttcgatca ctgccttgtc atcatcatag gtgatcattt gccgggaggc cgtgttgacc
      241 agatagggct gtttgcagac atcgttccat tgatagacga atccgttatc tggcatccct
      301 tgtatatact gattgtacag gcctgtgtag ctcttgtccg cgccattgcc attggtcttg
      361 taattgccaa gcccggtata attgctgaag acccagccaa aaaacggcac gccgatcacg
      421 atctgcagcg gattcaccct cctggtattc cttagatagt caacagtcgt aacaatggcg
      481 gtcgaggttc ctgtgccggc atcggtataa atcgggctgt taaagccaaa ccgggtggtc
      541 caggaggtgg caaattcata ggtcatcggg gaaaaccagt tgcagcactt tgccagcctg
      601 gcaaagtcga aatacttgcc cgcccaatcg ccggtgggga ccgccatggt gatgaacatt
      661 tcccgtttgg ataatttggc ggattttaca aaggccaccc ggagttcctg ggcaaaatcg
      721 gtaataccca atccatcctc ggaagaagca ggatattcat aatcgagatt gacgccgtca
      781 taatttcgct gctcgcaaag cttgaccagg ttgctgataa actgcttgcg atacttttct
      841 atggcaaaaa ttaccgccag gtctctggca ttctggccct gcctgcctgc cacgccgatc
      901 agaaccttga cattggccgc atgtgctctg gcaatcaccc gatcgccata gccaagatgg
      961 gttttatccg caagaccagg atacagacta taggccgctt tttcatactc gaggtcgcca
     1021 taggtgagcg cagggtcgga tgatgaggtg ggccgcaaaa aagaatagac aatatgggtg
     1081 acactggtat aatcaatgtc ctcccaggaa tagctgtacc atttggaagg ggttaaatag
     1141 ccctccacca ccctgttact gttgatgacg acaaaagtgg aatctttcgg cggggtgtag
     1201 gcgatggtgg aaggaggggg ctcaacaggc ccacccgatt cggagcagcc taaaaccgca
     1261 acaccggcga tcagggcgag cagcaaagag caaacagtat tgtgtacctt catccgtcct
     1321 cctttctgtc aatgtgagag aagaacctca gcctgtcagt tttccggggc gggagattgc
     1381 ctgaaccgaa tctggattgc aagcccgatt caccccgccg gagagatgcg ccggcatcat
     1441 gccctggcgg ctttgcgaag cagcggcgtc agccgcggat ccactcaaaa aaccccatcc
     1501 tctccagccc agcgcgcagg gccttgagct gctccgcgga gggattgcca ttgggcaggc
     1561 gggcggggcc gacctccaca ccgagccagc ccatcagggc cttggccgcg cccaggtagc
     1621 cgaccgaggc gagcgctttg atcagctgca ccgatttcca ctgctcctcc cgcgccgcgg
     1681 cgaggtcgcc ggcctcgaag gcccggatca gccgctgata gaccggcgcg gcaaagttga
     1741 acgaactgcc caccgcccct ttcgccccca gcgccagcgc cccgagcatg aattcatcca
     1801 cgccccacgg cacatcgaat ccgcccccgc ccgcgcgcag ggtgagctgg taagccatca
     1861 ggtcgctgtt cgtgaatttt attccggcca gggtcggaat cgcggcctcc cccctctcca
     1921 ggaactcatc catggcaaaa ttcacattgg tcaatgcagg aatatcgtaa aagtagaacg
     1981 gagtagacgg cgcgccggca gcaatcccgg cgcagcaggc aatgaggcgg tcgagcgtgt
     2041 ttggacgaaa atagcccggc gccagggcgg cgatggccag gacgccgagt tcttcggcct
     2101 gttcagccag ggcgcgcgcg tcgcgcaggc agttcgagcc gacatgcacg atcaggcgca
     2161 tgggggtgcc cttgatgacc tccgcccagc gttcggcgag ctgccggcgc tcgtccgtgc
     2221 cgaccgaatg gctctcgccg gtgctgccgc cgatgaagac ctgggtgacg ttgttgcgca
     2281 gcaggtgctc gcactgcttc tcgacagccg ccaggttcag cgcgccgtct gccgtgaagg
     2341 gggtgtgcgc ggcggcggtc aggccgtgca gggggtatcg ggttttcatc ttctctccct
     2401 atgggtaata tcctcctccg gcacggttgg aatctcctcc agcagcttcc agcagtacca
     2461 gagcatgcgc ggcagatgga aaaagccctt ccagtggttg cccttgagcg gcatggagac
     2521 gcgcccgtcg cggtggaggt agccgtacca ctcgctgtat tcggggtcgg gaaaatgggc
//
LOCUS       DAOPWU010000439         2568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_42293_length_2568_cov_3.989256, whole genome shotgun sequence.
ACCESSION   DAOPWU010000439 DAOPWU010000000
VERSION     DAOPWU010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42293_length_2568_cov_3.989256"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1072
                     /locus_tag="PKM23_14810"
     CDS             161..1072
                     /locus_tag="PKM23_14810"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY92793.1"
                     /translation="MKLKTNILGTLSLLMLLQAAGARGQWESGFAVLQIGAGARAAAM
                     GEAFAAVAGDASASFWNPAGAAGVNSFHAHLAHNEWIQEVSHDAAAVLFPLKPFHFGL
                     HAVVVSVPGIEQRLYPTEDPLSTFSAHDVVLGATFARRLGEDLALGLNLRYLNEKIHT
                     ESASGFSIDLGAHYRTPLRGMTAGLVVQHLGATRAMAKEEVTLPRSLRAGLAYTLPLG
                     SEEAPWLVSAEYVSIRHQSGHLHVGAEIRPLEMLALRAGYQTGYASRDFSAGFGVHTG
                     RFWIDYAFVPFQEELGRAHRFSLSIGS"
     gene            1165..2283
                     /locus_tag="PKM23_14815"
     CDS             1165..2283
                     /locus_tag="PKM23_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HNY92794.1"
                     /translation="MAKYKIALLPGDGIGKDVMDAAQIVLDKLGLDAEYTQGDIGWEF
                     WCREGDPLPKRTTELLRQTDCALFGAITSKPKEEAEAELAPELKGKGFVYASPIVRLR
                     QEFNLRTNLRPCKAYKGNPLNFRDDIDIVVFRENTEDLYAGVEFHPLPAEVMETLVRN
                     NKKMKRFESVPLEDIAVSLRINTRQGCRNIIRDSFEYAKKYGYKTVTVVEKPNVVRET
                     SGLMVREARKIAAEYPGIELWEANIDAMCMWLIKNPQDYGVLVTSNLFGDIISDLCAQ
                     LIGGLGFSSSGNIGDNYAVFEPTHGSAPKYAGQYKVNPMAMLFTVKLMLDWLGESRMA
                     ERLEKACAGVIAEGVVRTYDMGGTNTSLDVASAVAARL"
     gene            2380..>2568
                     /locus_tag="PKM23_14820"
     CDS             2380..>2568
                     /locus_tag="PKM23_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92795.1"
                     /translation="MNKNILRFLLLASIGLVIGIAFTRIGHNEAYPQENIRSELDRFV
                     EVFAYVKRYYVEPVESDKL"
BASE COUNT          506 a          748 c          801 g          513 t
ORIGIN      
        1 agctcacctt cggcctcgga tgcaatctgc agatctacgg caagaagatg gcgcttaact
       61 acgcctatgt cgcaccgggg gaggggccgc gcgccgatca tatcttttcg tggcatgcga
      121 gcctgtaggg atgaatgata ataaccggac gggaaggacg atgaaactta aaaccaatat
      181 tctggggacg ctttcgctgc tgatgctcct gcaggccgcg ggggcgaggg ggcagtggga
      241 gagcggtttt gcggtgctcc agatcggcgc cggcgcccgg gctgcggcca tgggcgaggc
      301 ctttgccgcc gtggcggggg atgcctccgc ctctttctgg aatcctgcgg gagcagccgg
      361 ggtgaactcc ttccacgccc atcttgccca caatgagtgg attcaggagg tgagccacga
      421 tgcggcggca gtgctctttc cgctcaagcc cttccatttt ggcctgcatg ccgtcgtcgt
      481 ctcggtgccg ggcatcgagc agcgtctcta cccgaccgag gatcccctca gcaccttttc
      541 ggcccatgat gttgtcctgg gggccacttt cgcccgcagg ctgggtgagg atctggccct
      601 cgggctcaac ctgcgctatc tcaacgaaaa gatccacacc gagagcgcca gcggcttcag
      661 catcgacctg ggcgcgcact accggacacc gctgcggggt atgaccgccg ggctggtggt
      721 gcagcacctc ggggcgaccc gggccatggc caaagaggag gtcaccctgc cgcggagcct
      781 gcgcgcaggc cttgcctaca cccttcccct cggcagcgag gaggccccct ggctggtgag
      841 cgccgaatat gtctccatcc gccaccagag cggtcatctg catgtcggcg cggagatcag
      901 gccgctggag atgctggcgc tgcgggcggg ctatcagact ggctatgcat cgcgcgactt
      961 tagtgccggc tttggcgtgc ataccggccg cttctggatc gactatgcct ttgttccctt
     1021 tcaggaggag ctggggcggg cgcaccgttt ttcattatcc atcggctcct gatttttttt
     1081 cgcttgcttc ctcatggaaa attaagtaaa ctataatttt agaatactct tcaaatacca
     1141 acctgaagaa agaggacgag aacgatggcg aagtacaaaa tagcgctgct gccgggtgat
     1201 ggcatcggca aggatgtcat ggatgccgct cagatcgtat tggacaaact ggggctggac
     1261 gcggaatata ctcagggcga catcggctgg gagttttggt gccgggaggg cgatcccctg
     1321 cccaagcgga cgaccgaact gctcaggcag accgactgcg ctctcttcgg cgccatcacc
     1381 tccaagccca aggaagaggc cgaggctgag ctggctcccg agctgaaggg caagggtttc
     1441 gtctacgcca gtccgatcgt gcggctgcgg caggagttca acctgcgcac caatctgcgt
     1501 ccctgcaagg cctacaaggg caatcccctc aatttccgcg atgatatcga catcgtcgtt
     1561 tttcgcgaaa acaccgagga tctctacgcc ggcgtcgaat ttcatcccct gcctgcggag
     1621 gtgatggaga ccctggtccg gaacaacaag aagatgaagc gcttcgagtc ggtgcccctc
     1681 gaggatatcg cagtttcgct gcgcatcaac acccgtcagg gctgccgcaa catcatccgc
     1741 gactctttcg agtatgccaa aaagtacggc tacaagaccg tgacggttgt ggagaagccc
     1801 aatgtggtgc gtgagaccag cggcctgatg gtgcgcgagg cgcgcaagat cgccgcggaa
     1861 taccccggca tcgagctctg ggaggcgaac atcgacgcca tgtgcatgtg gctgatcaag
     1921 aatccccagg attacggggt gctggtcacc agcaatctct tcggcgatat catctccgat
     1981 ctctgcgccc agctgatcgg cgggctgggc ttcagctcca gcggcaacat cggcgataat
     2041 tatgcggttt ttgagccgac gcacggttca gcgcccaaat acgccgggca gtacaaggtc
     2101 aatcccatgg cgatgctttt cacggtcaag ttgatgctcg attggctcgg ggagagccgg
     2161 atggcggaac ggcttgagaa ggcctgcgcg ggggtgatcg ccgaaggggt ggtgcgtacc
     2221 tacgacatgg gcggcacgaa cacaagcctg gatgtggcca gcgccgtcgc cgcccggctc
     2281 tgatccggat cccgggagga gcgctgcgga cttttcagcc ggccttctcc cctgatgggc
     2341 agggtggaac atggttcagc atgagaagaa atgagcaaaa tgaataaaaa catactccgt
     2401 tttttactcc tggcgagcat cggcctggtg atcggcatcg cctttacgcg catcggccac
     2461 aacgaggcct atccgcagga gaatattcgt tcggaactcg atcgttttgt tgaggtcttc
     2521 gcctacgtca agcgctacta cgtcgagccg gtggagagcg acaagctg
//
LOCUS       DAOPWU010000440         2563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_42413_length_2563_cov_5.187799, whole genome shotgun sequence.
ACCESSION   DAOPWU010000440 DAOPWU010000000
VERSION     DAOPWU010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42413_length_2563_cov_5.187799"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..388)
                     /locus_tag="PKM23_14825"
                     /pseudo
     CDS             complement(140..388)
                     /locus_tag="PKM23_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            complement(397..1062)
                     /gene="eutL"
                     /locus_tag="PKM23_14830"
     CDS             complement(397..1062)
                     /gene="eutL"
                     /locus_tag="PKM23_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HNY92796.1"
                     /translation="MAILDPIKARPLAVRLISSVDAGLHAELKLKPEQRSLGLITADI
                     DDALYVSIDEATKKAEVEVVYAKSFYAGAAHASGPLSGEIIAMLAGPNPGEVQAGIQA
                     ALDYLENEAIWYSANEANSLTFFPHLIARTGSYLSGVAGITPGEPLSYLIAPPLEATY
                     ALDQAMKAANVRLCLYFAPPSETNFSGGLLTGTQAACKAACDAFQETVLDIARNPIYF
                     MLG"
     gene            complement(1093..1983)
                     /gene="eutC"
                     /locus_tag="PKM23_14835"
     CDS             complement(1093..1983)
                     /gene="eutC"
                     /locus_tag="PKM23_14835"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HNY92797.1"
                     /translation="MDQKQIEAIVNAVLQEMNSPAAAPRPLQSAGAAPAPAPSTAGEG
                     PLPDLAGEEVKRWIGIGQPNNAKALGDLRAATNARVAVGRTGTRSRTLPYLRFLADHS
                     LSRDTVFKEIPEEWTTRTGLFIVQTQVSDKDEYLTRPDLGRMLSEEGRRLISEKCIHQ
                     PQVQIVLSDGLSTDAMTANYDEILPPLVKGFESAGLKVGTPFFVKYGRVKVEDEIGEH
                     LQADVVVLLIGERPGLGQSESMSAYMVYRPAVATTVESDRTVISNIHKLGTPPVEASA
                     VIVDTVKKMIAQKTSGINLK"
     gene            complement(2002..>2563)
                     /gene="eutB"
                     /locus_tag="PKM23_14840"
     CDS             complement(2002..>2563)
                     /gene="eutB"
                     /locus_tag="PKM23_14840"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006229882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HNY92798.1"
                     /translation="AYEVGKKYCRIAGSSMLYFETGQGSALSAGAHNGADQVTLEARN
                     YGLARRYRPFLVNTVVGFIGPEYLYDGRQITRAGLEDHFMGKLHGIPMGADACYTNHA
                     NTDQNALENLTVLLAAAGINYVMGVPLGDDIMLNYQTNAYHDTATIRATLGLRPAPEF
                     EAWLEKMGIMKNGMLTEKAGDASIFF"
BASE COUNT          443 a          706 c          902 g          512 t
ORIGIN      
        1 gtttgggttt ttcaggttgc ggggttttag ccggcggctg gccgccccgg ccctgaccgc
       61 tcccgccctt gcccgagccc tttctctggc ccatgccctt gcgcttcttg ctgatcaact
      121 cctcgaccag aaagtcggtg tctccatcag ggcgcgggat gacatgggcc gagatcagtt
      181 cgccgaagcg gatggccgcg gcggagccgg cgtcgacagc cgcctggcag gcggcgaggt
      241 caccctcaca gacgatggtc accagggccc gcccagggtt gtgctgtttg atcaggcgca
      301 cgcgggcggc cttgaccatg gcatcagctg actcgatggc gcagatcagc cctctggttt
      361 caataaagcc gtaggagagt ccagccataa aaagcctcag cccagcatga agtagatggg
      421 attgcgcgcg atatcgagta cggtctcctg gaaggcatcg caggcggctt tgcaggcggc
      481 ctgggtgccg gtgagcagac ctccggaaaa gttggtctcg gagggcggcg caaaatagag
      541 gcagaggcgc acgttggcgg ccttcatcgc ctggtcgagg gcgtaggtgg cctcgagcgg
      601 cggtgcgatc agataggaga ggggctcacc gggagtgatg ccggccacac ccgagagata
      661 actgccggtg cgggcgatga ggtgcgggaa gaaggtcagg ctgttggcct cgttggctga
      721 ataccaaatc gcttcgtttt cgagatagtc gagggcggcc tggatgccgg cctggacctc
      781 gccgggattg gggccggcca gcatggcgat gatctcgccg gagaggggcc cggaggcgtg
      841 cgcagcgccg gcgtagaagg atttggcata gaccacttcg acttcggcct ttttggtcgc
      901 ctcatcgatg ctgacgtaga gggcatcatc gatatcggcg gtgataaggc caaggctgcg
      961 ctgttcgggc ttgagtttga gctcggcatg gagtcctgca tccaccgagg agatcaggcg
     1021 gacggcgagg ggccgggctt tgatgggatc gaggattgcc ataatttctg tcctgtcgct
     1081 agagtataca cattatttca ggttgatgcc actggttttt tgggcgatca ttttcttgac
     1141 ggtatcgacg atcacggccg aagcctcgac cgggggggta ccgagcttgt gaatgttcga
     1201 gatcacggtc cggtccgact cgacggtggt ggcgaccgcg gggcggtaga ccatgtaggc
     1261 gctcatgctc tcagactggc cgaggccggg gcgctcgccg atgaggagga ccaccacgtc
     1321 ggcctgaagg tgctcgccga tctcatcctc caccttgaca cggccgtatt tcacaaagaa
     1381 gggggtcccc accttcaggc cggccgactc aaagcccttg accagggggg gaaggatttc
     1441 atcatagttg gcggtcatgg cgtccgtgga aaggccgtcg gagagaacga tctgcacctg
     1501 gggttggtgg atgcattttt cgctgatgag cctgcgcccc tcctccgaga gcatgcggcc
     1561 gagatcgggc cgggtcaggt attcgtcttt gtcggagacc tgggtctgca cgatgaacag
     1621 gccggtgcgt gtggtccact cctcggggat ctccttgaag acggtgtcgc gcgacaggga
     1681 gtgatcggcg aggaagcgca gataaggcag ggtacggctg cgggtgccgg tgcggccgac
     1741 ggcgacgcgg gcattggtgg cggcgcgcag atcgcccagg gccttggcgt tgttgggctg
     1801 cccgatcccg atccaacgct tgacctcctc cccggccagg tcgggcaagg ggccctcgcc
     1861 ggctgtggag ggggcgggcg caggcgctgc gcctgcactc tggagaggac ggggggcggc
     1921 cgccggcgag ttcatctcct gcagcaccgc gttgacaatg gcttcaatct gtttttgatc
     1981 catgattatc ggtctccaga tctagaaaaa tatcgacgca tcgccggcct tttcggtcag
     2041 cataccattt ttcatgatgc ccatcttttc gagccaggct tcaaattccg gggcgggacg
     2101 cagtcccagg gtggcgcgga tggtggcagt gtcgtgatag gcgttggtct gatagttgag
     2161 catgatgtca tcgccgaggg gaacgcccat gacgtaattg atacctgctg cggcgaggag
     2221 gacggtcagg ttctcgagtg cgttctggtc ggtgttggca tggttggtgt agcaggcatc
     2281 ggcccccatc ggaatgccgt ggagtttgcc catgaaatga tcctcgagtc cggcgcgggt
     2341 gatctggcgg ccgtcataga ggtactcggg cccgatgaag ccgaccacgg tgttgaccag
     2401 gaaggggcga tagcgccggg ccaggccgta attgcgcgcc tcgagggtga cctgatcggc
     2461 gccgttatgg gccccggcgg agagggccga accctggccg gtttcgaagt aaagcatgct
     2521 cgagccggcg atgcggcagt atttcttgcc cacctcatag gcc
//
LOCUS       DAOPWU010000441         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_42728_length_2550_cov_3.721443, whole genome shotgun sequence.
ACCESSION   DAOPWU010000441 DAOPWU010000000
VERSION     DAOPWU010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42728_length_2550_cov_3.721443"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PKM23_14845"
     CDS             <1..721
                     /locus_tag="PKM23_14845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY92799.1"
                     /translation="WGYMYGNTPHHQVFLELGRSLSAASGYEYGNWAMGLLYGTNGDA
                     DDYFYGASREAHPCFSVTIEVGTSFHPPEEEMSQLIAENRPASLYIAYIADKLAGNPW
                     LIVPADSAGSALTTAVAAADEPEQQPKAFALLRSYPNPFNAATTISFTLTEAAAIRLT
                     LLDALGRPLRTLLQGFYQAGEHRLRLDAGGLPSGLYFCRLESSVSFAQVDAAAAGGRG
                     APASTQAGPRQSALLRILLLR"
     gene            784..>2550
                     /gene="mutL"
                     /locus_tag="PKM23_14850"
     CDS             784..>2550
                     /gene="mutL"
                     /locus_tag="PKM23_14850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNY92800.1"
                     /translation="MQKKSHIINVLPENLINKIAAGEVIDRPASVVKELVENAIDAGA
                     TWITLIIKEGGAQLIQVNDNGSGMSEEDAILSLQRHATSKIRTYADIEKILTLGFRGE
                     ALASIAAVSRLELRTVPKGAIEGTAIFVEGGVVQKVEGSGGNPGTSIAVKNLFFNTPA
                     RRKFLRAESTEYRSILAMMNHFTLAFPEIGFHVVNDDAEVYRLAPAALQERLTQVLGA
                     RVRNNLVRVEDKTTLMTISGYIGNYDAMRKSRNDQYLFLNRRFINDRTLSYAVTQAFG
                     ETIPKGHYPLFILFIDIDPERVDVNVHPTKNEVKFADSRLVFDLLRSAVKRALRTDHI
                     IPEIQQFYPPRWGAGGAPAGPSVSRGDIVKPDDQGSFDWESGEPFFPPPGLFPGGYPG
                     GAPWTIPSGSAPAGEADTGGADFGGFSQGGSSAGGTGAGEPAGEPAGEPAGEASGETA
                     TGGGSPAGGPFPIQNSGVSGPRPRETATVWQVQNKYIVSQIKSGLVVIDQHVAHERIL
                     YERAKKNITRTEGASQQLLFPQTLELSAQDYQVAVEIAPWLEKIGFLIKLFGGRTVVV
                     EGVPTGMRIGAENAILMNIIDQY"
BASE COUNT          475 a          913 c          756 g          406 t
ORIGIN      
        1 ctggggctat atgtacggca acacccccca tcaccaggtc tttctcgagc tcggccgcag
       61 cctcagtgcc gccagcggct acgaatacgg caactgggcg atgggtctgc tctacggcac
      121 caacggcgac gccgacgact atttttacgg cgccagccgg gaggcccacc cctgcttcag
      181 cgtcaccatc gaggtcggca cctcctttca tccgcccgag gaggagatga gccagctcat
      241 cgccgagaac cgccccgcca gcctctacat cgcatatatc gcggacaagc tggccggaaa
      301 tccctggctg atcgtcccgg ccgacagcgc cggatccgcg ctaaccacag ccgtcgccgc
      361 ggctgatgag ccggagcagc agccaaaggc cttcgccctg ctgcgcagct atcccaatcc
      421 cttcaacgcc gccaccacta tcagctttac gctgacggag gccgcagcca tccgtctcac
      481 cctcctcgac gccctcggac ggccgctgcg cacgctgctg cagggctttt accaggccgg
      541 cgagcaccgg ctccggctcg acgcaggcgg actgccgagc gggctctatt tctgccggct
      601 cgagagcagc gtttccttcg ctcaggttga tgcggcggca gccggcggga ggggcgcccc
      661 ggcctccacg caggccggtc cgcgccagtc cgccctgctc aggatcctcc tgctccgcta
      721 aattcccctt gaaaaagtgc atgaaattcg ctacactcta ttctaaacgg agcaagggag
      781 cgcatgcaga aaaaatccca catcatcaac gtcctgcccg agaacctgat caacaagatc
      841 gccgccgggg aggtcatcga ccgcccggcc tcggtggtca aggagctggt cgagaacgcc
      901 atcgacgccg gggcgacctg gatcaccctg atcatcaagg aggggggcgc ccagctcatc
      961 caggtcaacg acaacggcag cggcatgagc gaggaggatg ccatcctctc cctgcagcgc
     1021 cacgccacct ccaagatccg cacctatgcg gacatcgaaa agatcctcac cctcggcttt
     1081 cgcggcgagg cgctggccag catcgccgcg gtctcgcgcc tcgagctgcg caccgtgccg
     1141 aaaggggcga tcgaggggac ggccatcttc gtcgagggcg gcgtggtgca aaaggtggag
     1201 ggctccggcg gcaatcccgg cacctcgatc gcggtcaaga atctcttttt caacaccccg
     1261 gcgcggcgca aattcctgcg cgcggagagt accgagtacc gctcgatcct ggcgatgatg
     1321 aaccatttta ccctcgcctt tcccgagatc ggctttcacg tcgtcaacga cgatgccgag
     1381 gtctatcgtc tcgccccggc ggcgctgcaa gagcggctga cccaggtgct cggggcgcgc
     1441 gtgcgcaaca acctggtccg ggtcgaggac aagaccaccc tgatgaccat cagcggctat
     1501 atcggcaact atgacgccat gcgcaagtcg cgcaacgacc agtacctctt cctcaaccgc
     1561 cgcttcatca acgaccgcac cctcagctac gcggtcactc aggccttcgg cgagaccatc
     1621 cccaaggggc actatcccct ctttatcctc ttcatcgaca tcgaccccga gcgcgtcgac
     1681 gtcaacgtcc atccgaccaa gaacgaggtc aagttcgccg attcgcgact ggtcttcgac
     1741 cttttgcgca gcgcggtcaa gcgcgccctg cgcaccgacc acatcattcc ggagatccag
     1801 cagttctacc ccccacgctg gggggccggc ggcgcgcccg cgggcccatc cgtcagccgt
     1861 ggcgatatcg tcaagcctga cgaccagggc tccttcgact gggagagcgg cgagccgttc
     1921 tttcccccgc ccgggctctt tccgggcggc tatcccggcg gcgcgccctg gacaataccc
     1981 tccggctccg cgcccgccgg tgaggccgat acgggcggag cggattttgg cggattcagt
     2041 caaggcggtt cgagcgccgg cggaacgggc gcaggcgagc ctgcagggga gcctgcaggc
     2101 gagcctgccg gtgaggcctc cggtgaaacc gccaccggcg gcggcagccc ggccggcggc
     2161 ccctttccga tccagaacag cggcgtctcc gggccgcggc cgcgcgagac cgccacagtc
     2221 tggcaggtgc agaacaaata catcgtctcg cagatcaaga gtggcctggt ggtgatcgac
     2281 cagcacgtcg cccacgaacg cattctctac gaacgcgcca aaaagaacat cacccggact
     2341 gaaggggcct cccagcagct gctcttcccc cagaccctcg aactctccgc ccaggactat
     2401 caggtggccg tcgagatcgc cccctggctg gaaaagatcg gctttctgat caagctcttc
     2461 ggcgggcgca ccgtggtcgt cgagggggtg cccacgggga tgcgcatcgg cgcggagaac
     2521 gccatcctga tgaacatcat cgaccagtac
//
LOCUS       DAOPWU010000442         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_42888_length_2543_cov_3.485129, whole genome shotgun sequence.
ACCESSION   DAOPWU010000442 DAOPWU010000000
VERSION     DAOPWU010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42888_length_2543_cov_3.485129"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKM23_14855"
     CDS             <1..189
                     /locus_tag="PKM23_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92801.1"
                     /translation="AMKAKITARDFRKGNAGKAETTLTIVYSADLRILLWIAMLFIKI
                     GTKIMNCRAVLTEIKKEG"
     gene            253..504
                     /locus_tag="PKM23_14860"
     CDS             253..504
                     /locus_tag="PKM23_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92802.1"
                     /translation="MKQTYIKLGERWVKGKKCPCGKMFTEEPLHHYDYAYAPEVCPKC
                     GTGRKKFLDGAILVERGETRYIFGIKKNDHWKLIEFKEF"
     gene            504..1448
                     /locus_tag="PKM23_14865"
     CDS             504..1448
                     /locus_tag="PKM23_14865"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNY92803.1"
                     /translation="MPSYICTVCGNTVTYPAGDQALHEFRRYECAHCYTIIHSRRFKK
                     PKPGATVMFGPAGPGIGDYIFKNFVMEQFSRLYPETDIINVMGGSNVAAQIGADLIFW
                     ADNAGIKNPAPPGAINYIITNEVLGFVRDGFWPRLWFELQEYELPRGVDLRNSVVVNL
                     RNIPRCDPKNVTDLEADNLYKIMVHLKARGEIDRIILVGNDAPLTMSWLPEFAIDLRN
                     QLTLPEIAWLCRGAMFTVGKDSGILHIAAAAGGYVIGWGYRDSHWRPLAPEGRVTAIM
                     EQPSYKYKLAEAVDITVNHRMICTAQYLPENLPKWCRG"
     gene            1628..1813
                     /locus_tag="PKM23_14870"
     CDS             1628..1813
                     /locus_tag="PKM23_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92804.1"
                     /translation="MPEQPKREREQAYDVEGIAGILRQLVSMQTKATIELRHDGSGKD
                     IFTFYFTKINAADIALK"
     gene            1918..>2543
                     /locus_tag="PKM23_14875"
     CDS             1918..>2543
                     /locus_tag="PKM23_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92805.1"
                     /translation="MPDMLKRVIEYAIPQIKAVKATDEEWELIKSFMVDPEGFAPEDV
                     KTFKFHLAHNFVDRDGERFTIGTLNQFAKTIVGKEFLIAHEWGPPGEGKFYGAELSRV
                     SIDEAAAIASDHPDQEGLRKQLEKIAGIDGGIAFLDVKMYMLADSPWARKIGAGINTH
                     VSIGFSAGRAVEVMDGETVLWREYKYQGGAKRTEALEGSLVFLGSQYGA"
BASE COUNT          629 a          643 c          727 g          544 t
ORIGIN      
        1 gccatgaaag caaaaattac cgctcgcgat ttcaggaaag gtaatgcagg caaagcagaa
       61 acaactttga cgatcgtgta ttctgccgac cttcggatat tgctttggat cgcgatgctt
      121 tttattaaaa tcgggacaaa gatcatgaat tgcagggccg tcctgactga aattaaaaag
      181 gagggctgat gcttaattta tgcataggta ccggctggtg cgcccataaa gaggggcaca
      241 acagggatta acatgaaaca gacttatata aaacttggcg agcgatgggt aaagggcaaa
      301 aaatgcccct gcgggaagat gtttactgaa gaacccctgc atcattatga ctatgcatac
      361 gctccagagg tatgtccgaa atgtggcacg ggcaggaaaa agtttctgga tggcgccatc
      421 cttgttgagc gcggtgagac gcgttacatt tttggtatca agaaaaatga tcattggaaa
      481 ctcatagagt tcaaggaatt ctgatgccct cctacatctg cacggtatgc ggcaacacgg
      541 tcacctatcc ggcaggggac caggccctgc acgagttcag gcgctacgag tgcgcgcact
      601 gctacaccat catccattcc cggcgcttca aaaagccaaa gccaggcgct acggtaatgt
      661 ttggccctgc cggcccgggg ataggggact atattttcaa gaattttgta atggagcagt
      721 tttcccgcct ctaccctgag accgatatca tcaatgtcat gggtggctcc aatgtggccg
      781 cacagatcgg cgctgacctc atcttctggg ccgacaacgc tggcatcaag aaccccgctc
      841 ccccaggggc gatcaattat atcatcacca atgaggtgct gggctttgtc agggacggat
      901 tttggccgcg gctttggttt gagctccagg agtacgagct gcccagaggg gtggacctgc
      961 gcaactctgt ggttgtgaac ttgcgcaata tcccccgctg tgatccgaaa aacgtcacag
     1021 accttgaagc ggataatctc tacaagatta tggtccacct taaggcccgc ggggagatcg
     1081 accggatcat cctggtgggg aatgacgctc ccctgaccat gagctggctt cctgagtttg
     1141 ccattgacct cagaaaccag ctcaccctcc cggaaatcgc ctggctctgc aggggcgcga
     1201 tgttcaccgt cggcaaggac tccgggatcc tgcatatcgc agcggcggcc ggcggctatg
     1261 tgatcggctg gggataccgc gacagccact ggcggccgct agctccggaa gggcgggtaa
     1321 cagcgataat ggagcaaccg agctacaagt acaagctggc tgaggccgtg gacataaccg
     1381 taaaccatag aatgatttgc acggcacagt atttgcccga gaacctacca aagtggtgca
     1441 ggggataaat tgagggcttg tgatatgcct tttgcggtct gcgataaatg cggaaaacaa
     1501 ttctgttatc cagtgggtga caaatccgtt cacaattgcg tcagaactgg ctctgtgcct
     1561 gaatggcaag agactcagac gcagcagccg aaatattttc aggacgaatc cgttgaggtg
     1621 aagaaggatg ccggagcagc cgaagaggga gagggaacag gcgtacgacg tcgaagggat
     1681 cgccgggata cttcggcagc tggtatccat gcagacgaaa gcgacgattg agcttcgcca
     1741 cgacggcagc gggaaagaca ttttcacttt ttattttacc aagatcaacg ctgccgatat
     1801 cgccctgaag taatttgaca tatttttagc gcggtaacca aacaaacggg ccgctgcaac
     1861 catgggcatt ctcctgcctc ctggttgtgg cggccctttc tattttaggg acaaacgatg
     1921 cctgatatgc tgaaacgagt gatcgagtac gctatcccgc agatcaaggc ggtaaaggcc
     1981 accgatgagg aatgggagct gatcaagagt ttcatggtcg atcctgaagg gtttgctccg
     2041 gaagatgtca agaccttcaa gtttcatctg gctcacaact ttgtcgaccg ggacggcgag
     2101 cgcttcacca tcggcaccct caatcagttt gccaagacca ttgtgggcaa ggagttcctg
     2161 atcgcccatg agtgggggcc tcccggtgag ggcaagttct atggcgcaga gctctccaga
     2221 gtctccatcg acgaggccgc ggccatcgct tctgaccacc cggaccagga aggcctcaga
     2281 aagcagctcg agaaaatcgc cggcatcgat ggcggtattg ccttccttga tgtcaagatg
     2341 tacatgctgg ctgacagccc ttgggcgcgc aagatcggcg ccggcatcaa cacacatgtg
     2401 tcaattggat tttccgctgg acgcgccgtc gaggtcatgg acggagagac ggtcctgtgg
     2461 cgggaataca agtaccaagg cggagccaag cggaccgagg ccctggaagg atcgctggtg
     2521 tttttgggca gtcagtacgg cgc
//
LOCUS       DAOPWU010000443         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_42894_length_2543_cov_3.030547, whole genome shotgun sequence.
ACCESSION   DAOPWU010000443 DAOPWU010000000
VERSION     DAOPWU010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42894_length_2543_cov_3.030547"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="trkA"
                     /locus_tag="PKM23_14880"
     CDS             complement(<1..937)
                     /gene="trkA"
                     /locus_tag="PKM23_14880"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNY92806.1"
                     /translation="MKVIVIGAGLVGTYICEYLSSEDHDVTVIEKDPAVCRKATDTLD
                     AMVIQGDGASPALLVKAGLHKTDMMVAVSTDDEVNILACLAAAKAGVKIKIARLRNPE
                     FSQEGTLLGKQELGIDFIIHPERETARELVWLTRRSAATDVIEFEEGKVQLIGVRLDA
                     SSPVLNKTLQQIDRENPDLLFRAVAIFRANKTIIPGGEDHVTRGDQLFFITKTELVPQ
                     LLEVLGKSDERIEKVMILGGGRVGRIVAAELEKDKECQVKLVESNREKSQRIAEELQR
                     TLIIVGDGTDLDLLAVEGIMEMDSYIAVTSDEETNI"
     gene            1280..1624
                     /locus_tag="PKM23_14885"
     CDS             1280..1624
                     /locus_tag="PKM23_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY92807.1"
                     /translation="MSSFSVRSQLCAGILIIQAGGYLNREGGEAISQAAALPAFAAVN
                     RVVLQLAGVEAANSVGIAHLLEMQERLMARRGGLALVGASPELTRTFSIMGLYTHAFK
                     AESIREALALLS"
     gene            1694..>2543
                     /locus_tag="PKM23_14890"
     CDS             1694..>2543
                     /locus_tag="PKM23_14890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNY92808.1"
                     /translation="MDRHLKKFFELALDLFCIADAGKGRFIEVNPAFSRSLGWTREQL
                     LAQPFWELIHPDDHQKTAAEVEHMRQGVPTRSFENRFRSVRGDYRHLVWNVFPEPASD
                     LLFAAARDVTEKKLNERELRKLAAKLQQKILQLHELIDTGIDIIRIDQSESLIQLALR
                     RVTALTDATRGLVLVRKPDGATEQIAFPDYAGAGGEESVDIIQSGFECLGHQYTFMAI
                     GKENRHNSARFETVDKMLLHLVSQQVRTALENRFYLNEMIEKQRLLREVELASVIQKT
                     ILPTELP"
BASE COUNT          470 a          816 c          742 g          515 t
ORIGIN      
        1 ggatgttggt ttcctcgtcg ctggtgacgg cgatgtagct gtccatctcc atgattccct
       61 cgacggcgag gaggtcgaga tcggtgccgt cgccgacgat gatcagggtg cgctgcagct
      121 cctcggcaat gcgctggctt ttctcgcgat tggattccac cagcttgacc tggcactcct
      181 tgtccttctc cagctcggcg gccactatac ggccgacccg cccgcccccg aggatcatca
      241 ccttctcgat gcgctcatcg ctcttgccca gaacctcgag cagctgcggc accagctcgg
      301 tcttggtgat aaaaaagagc tgatccccgc gtgtgacgtg gtcctcgccg ccgggaatga
      361 tggtcttgtt ggcgcggaaa atggccacgg cgcggaagag gaggtcgggg ttctcccggt
      421 cgatctgctg cagggtcttg ttcagcaccg gggaggaggc gtccagccgc accccgatca
      481 gctgcacctt gccctcttcg aattcgatga catcggtggc ggctgagcgc ctggtcagcc
      541 agaccagctc gcgcgcggtt tcgcgctcag ggtggatgat aaaatcgatg cccagctcct
      601 gcttgccgag cagggtcccc tcctggctga attcaggatt gcgcagccgg gcaatcttga
      661 tcttgacccc ggccttggcg gcggccaggc aggcgagtat gttgacctca tcgtcggtgc
      721 ttaccgccac catcatgtcg gttttatgca gccccgcctt gaccagcagc gcgggtgagg
      781 ccccgtcccc ctggatcacc atggcatcga gggtgtcggt cgccttgcgg cagaccgccg
      841 ggtccttctc gataactgtg acatcgtggt cttccgaact gagatattcg cagatgtagg
      901 tgccaacaag gccggcgccg atgacgatca ctttcatgct gtcgggattt tctccttgct
      961 tccggattcc gcctccgcca ctccggggta tctgcggatt cggttttctt attccgcggc
     1021 ctaatctaat cttaatttaa ggaaaatgca agctgaatgt gaccgggcag gcaccttcgg
     1081 ctggggtgcg aacctgaaga ggcagtgctg gttctggaat accccgcccg ccgcggggaa
     1141 gggagtgccg tattattcgg cgcgcctgct ttttccggcg gggggagaaa taattgcgcc
     1201 cgccttccgg ttaaactcct ataaggcact tgccaagcca caatatttcc ccgaccaaag
     1261 gatggtgaac acgacacgca tgtcctcatt ctccgtcaga agccagctct gcgccgggat
     1321 cctcatcatt caggcgggag ggtacctcaa ccgcgaaggt ggtgaggcga tttcccaagc
     1381 tgcggcgctt cccgcttttg ctgcggtcaa ccgggtggtt ctgcagctgg ccggtgtgga
     1441 ggccgccaac agcgtcggca tcgcccacct gctcgaaatg caggagcgcc tcatggcgag
     1501 gcgggggggg ctcgccctgg tgggagccag tcccgagctg acccggacct tcagcatcat
     1561 ggggctctat acccatgcct tcaaggcgga gagcatccgg gaagcccttg cactgctctc
     1621 ctgagcacgc cacaattccc tctcccgctg ctgcgcatgc gacgcggcga ccccggacct
     1681 gaaacgggca cttatggacc gacacctgaa aaaattcttc gaactggccc tcgatctttt
     1741 ttgcatcgcc gatgccggta aggggcgctt catcgaggtc aaccccgcct tttcgcgatc
     1801 cctgggctgg acacgagagc agctgctcgc gcagcccttc tgggaactca tccaccccga
     1861 tgatcatcaa aagaccgccg ctgaggtcga gcacatgcgg cagggagtgc ccacccgctc
     1921 cttcgagaac cgctttcgca gcgtccgggg cgactaccgt cacctggtct ggaatgtctt
     1981 tccggaacct gccagcgatc tgctcttcgc cgctgcccgc gacgtgaccg agaaaaagct
     2041 caatgaaaga gagctgcgca agctggcggc caaattgcag cagaagatcc tccagctgca
     2101 cgaactcatc gacaccggca tcgacatcat ccgcatcgac cagagcgaat ccctcatcca
     2161 gcttgccctg cgccgggtca cggccctgac cgatgccacc cgcggactgg tcctggtcag
     2221 gaaaccggac ggtgcgaccg agcagatcgc tttcccggac tatgccggag ccggcgggga
     2281 ggagagcgtg gatatcattc aatccggttt cgaatgcctg gggcaccagt acacctttat
     2341 ggccatcggc aaggagaacc gccacaacag cgcccgcttc gagaccgtgg acaagatgct
     2401 cctccatttg gtcagccagc aggtgcgcac cgccctcgag aaccgctttt acctcaacga
     2461 gatgatcgaa aagcagcgcc tcctgcgcga ggtcgaactt gcctctgtta tccagaagac
     2521 cattctcccc accgaacttc cgg
//
LOCUS       DAOPWU010000444         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_43084_length_2535_cov_4.317339, whole genome shotgun sequence.
ACCESSION   DAOPWU010000444 DAOPWU010000000
VERSION     DAOPWU010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43084_length_2535_cov_4.317339"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKM23_14895"
     CDS             complement(<1..638)
                     /locus_tag="PKM23_14895"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNY92809.1"
                     /translation="MKKTAAAGIILLMAGLMLCQKPGGKGHLMIIGGGDKPMDAMQAF
                     VDHCQGKPVVIITSASSDLDYGQFTAGQLRQCGAAQIALRHIVEPEVANSDSITALID
                     AAGGVFFTGGDQDRLMNRLGGTRTEEALRRLYYERGGIIGGTSAGAAVMSEVMITGNE
                     LVNTDSTRNFFCIQKGNVETKRGFGFVRNAVIDQHFLMRKRHNRLIAVVLEHP"
     gene            complement(644..1753)
                     /locus_tag="PKM23_14900"
     CDS             complement(644..1753)
                     /locus_tag="PKM23_14900"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNY92810.1"
                     /translation="MDEQKLVTLFLELVRIDAVSLQERPVADFIKERLAALGLEAEED
                     DAGRKLNGSAGNLIVRLPGEDKNRAPLLLLAHMDTVHPTAGVKPALADGVLRSDGSTI
                     LGVDNRAGVTLILALIDELIERRLRHRTLEIVFTVGEELGMLGAMALDFSRLEAREGF
                     VFDCTAVPGGYVASTPTAYDFKIRITGKPAHSAVAPEKGINALTMATHVMSGFPVGRI
                     SDHSVANFGTIHGGTADNVVPDQVTLTGEFRSFSEEEISAMRELLTSLCSAAGERWGG
                     SCEPSFMLSFQGFQFGPEMPLVQRLHGAFARAGVAPQPLVYYGGSDANVFNQHGIKII
                     NTGIGANAPHSTDEHIRPADMLKGYEILSHLIEAE"
     gene            complement(1811..>2535)
                     /locus_tag="PKM23_14905"
     CDS             complement(1811..>2535)
                     /locus_tag="PKM23_14905"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNY92811.1"
                     /translation="GASEATIVRFAQLLGYRGYKELKETLSSSLKAQLSPTARYHLAV
                     AETGKSPDVLKLASRNVISNIHETIRSIDTHIFAAAVDSIIAADQIYCIGLELSSHLA
                     QLMTFLLRQYSYNAQHLSLDYFRYEEQVAFMNPRDLVIAFSFSPYSRETVDAIALAKK
                     RAVPSILFTDKKSSPARTWATHCLPIKTDNIMFSNSLGAVVTVLNAIITELNFRDKER
                     TLAALRIIEESIRDDRYFVAQT"
BASE COUNT          461 a          718 c          871 g          485 t
ORIGIN      
        1 gggtgttcga gcaccacggc gatgaggcgg ttgtggcgct tgcgcatgag gaagtgctgg
       61 tcgataacgg cgttcctgac aaagccgaat ccccgcttgg tctcgacgtt gcctttctgg
      121 atgcagaaaa aattgcgcgt cgaatcggta ttgaccagct cattgccggt gatcatcacc
      181 tcgctcatca ccgccgcgcc ggcgctggtg ccgccgatga taccgccccg ttcataatag
      241 aggcgcctga gggcctcttc agtgcgggtg ccgccgagcc ggttcatcag ccggtcctgg
      301 tcgccgccgg tgaaaaagac tccgccggcc gcatcgatga gagcggtgat gctgtccgag
      361 ttggcaacct ccggttcgac aatgtggcgg agagcgatct gcgccgcgcc gcactgcctg
      421 agctggccgg cggtgaattg gccgtagtcg aggtccgaac tcgccgaggt gatgatcacg
      481 accggcttgc cctgacaatg gtcgacgaag gcctgcatgg catccatcgg cttgtcgccg
      541 ccgccgatga tcatgaggtg gccttttccc ccgggcttct ggcagagcat gagcccggcc
      601 attaaaagaa tgatccccgc tgccgccgtt tttttcatcc ttgtcactcc gcttcgatca
      661 gatggctgag aatttcgtat cccttgagca tgtcggccgg acggatatgc tcatcggtgg
      721 aatggggcgc attggcgccg atgccggtat tgatgatctt gatgccgtgc tggttgaaga
      781 cgttggcgtc gctgccgccg tagtagacca ggggctgcgg cgccacgccg gcgcgcgcaa
      841 aggcgccatg aagccgctgc accaggggca tctccgggcc gaactgaaag ccctggaagg
      901 agagcatgaa agagggttcg caggagccgc cccagcgctc cccggcggcg ctgcacaggg
      961 aggtcagcag ctcgcgcatg gccgagatct cctcctcgct gaaagagcgg aactcgccgg
     1021 tcagggtcac ctggtcgggg acgacattat ccgccgtgcc gccgtggatg gtgccgaaat
     1081 tggcgacgga gtggtcgctg atccggccga ccgggaagcc gctcatcaca tgcgtcgcca
     1141 tggtgagggc gttgatcccc ttctccgggg cgaccgccga gtgggccggc ttgccggtga
     1201 tgcggatctt gaaatcatag gctgtgggcg tggaggcgac atagccgccg ggcacagccg
     1261 tgcagtcaaa gacgaaaccc tcccgcgcct cgaggcggga aaaatcgagg gccatggcgc
     1321 cgagcatgcc cagctcctcg ccgacggtga agacgatctc cagagtccgg tgccggagcc
     1381 gccgttcgat cagctcatcg atgagggcga ggatgagggt cacgccggcg cggttgtcca
     1441 ccccgagaat cgtgctgccg tccgaacgca gcacgccgtc ggcgagggcg ggcttgactc
     1501 ccgccgtggg atgcaccgta tccatatggg ccagcaggag caggggcgcg cggtttttat
     1561 cctcgcccgg cagccggact atcaggttgc cggcgctgcc gttgagtttg cggccggcgt
     1621 cgtcctcctc ggcctcgagg ccgagggcgg caagcctctc cttgatgaaa tcggcgacgg
     1681 gccgctcctg cagcgagacc gcatcgatcc ggaccagttc gagaaatagc gtgaccagct
     1741 tttgttcgtc catataccct cgtattttga aaaaacgggt acgaacagcg ggaattgaaa
     1801 gggcctggcc tcaggtctgc gctacaaaat agcggtcatc gcggatgctc tcttcgatga
     1861 tccggagcgc ggccagggtg cgctccttgt ccctgaaatt gagttcggtg atgatggcat
     1921 tgaggacagt gacgaccgcc cccagtgaat tgctgaacat gatattgtcg gtcttgatcg
     1981 gcaggcagtg ggtggcccag gtgcgggcgg gcgaactctt cttgtcggtg aagagaatgc
     2041 tgggaacggc gcgctttttg gccagggcga tggcatcgac ggtctcccgc gaatagggag
     2101 agaagctgaa ggcgatcacc aggtcccgcg gattcataaa ggcgacctgc tcttcgtagc
     2161 ggaaatagtc gagggagagg tgctgggcgt tatagctgta ttggcgcagg agaaaggtca
     2221 tcagctgcgc gagatgggat gagagttcca ggccgatgca gtagatctga tcggcggcga
     2281 tgatgctgtc gaccgcggcg gcgaagatgt gggtgtcgat ggagcggatg gtctcatgaa
     2341 tattggagat gacgttccgg gaggcgagtt tgagcacgtc cggagacttg cccgtttccg
     2401 ccacggccag gtgatagcgc gctgtggggg aaagctgggc cttgaggctg ctggagaggg
     2461 tctccttgag ctccttgtag ccgcgataac caaggagctg ggcgaagcgg acgatggtgg
     2521 cctcgctggc cccgg
//
LOCUS       DAOPWU010000445         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_43341_length_2524_cov_3.507898, whole genome shotgun sequence.
ACCESSION   DAOPWU010000445 DAOPWU010000000
VERSION     DAOPWU010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43341_length_2524_cov_3.507898"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PKM23_14910"
     CDS             complement(<1..1185)
                     /locus_tag="PKM23_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNY92812.1"
                     /translation="MKKIVFLILLLLPVALFAAEKRAFRIADLYALKSISDIQISPDS
                     KRVCFVVGSQDLAKSERNSDLWLMNLDGSGLRQLTFSPQGDYHPRWSPDGKTIAFLSG
                     RDQGGQIWLLPADGGEARRLTDLAVSPGDPEWLPDGGGFIFTTMVYPECGGDDDCSKK
                     ILETMEEGPVQAHLADRLFYRHWTWWRDGRVIHTLKYTLADSQLTDLTPGAIDQPADA
                     GAGGHAGFSLSPDGRELAIALHPDRNDYETTDKDVWIKSLVKGDSLNLTEDNTAYDGE
                     PVWSPDGRYIAYITQKVPVYEADRKRLAIYDRQNGIHRILTEPFDYWVNAFQWASDSR
                     SLYFTADVRGHVPLYQVDLQKGAIRQLLDLRHITGFRVAPDGRSVLCTRTSVGEPVEL
                     WRA"
     gene            complement(1294..2463)
                     /locus_tag="PKM23_14915"
     CDS             complement(1294..2463)
                     /locus_tag="PKM23_14915"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNY92813.1"
                     /translation="MAKLRVGVIFGGRSGEHEVSLVTATSIIEALDKNKYEIIPIGIT
                     KQGEWVAGENALAELKNHRPGLPALLSPDAGSHQLLMQKGASGLLPVEKLDVVFPALH
                     GPFGEDGTLQGLLELMDIPYVGAGVLGSAMAMDKVVQKHICRQQGIPTIDFLWFRDID
                     WRAEGGSEPFPALPDQLANRSREEIVGSLIDALGLPLFVKPPNLGSSLGISKAHDRRE
                     LFNAIEEALQYDRKVLVEKAVAQPRELELSILGNENPRVSVAGEVFPGNEFYDYDAKY
                     VDDNSGLEIPARLTPGQLRALQETAIRAYVATECEGMARADFLMEKETGRFYLSELNT
                     IPGFTRISMYPKMWEASGLPYSALLDELIELALRRQERRRTLRTSYEPKRDWYKG"
BASE COUNT          449 a          728 c          869 g          478 t
ORIGIN      
        1 ggcgcgccag agctcgaccg gctcgccgac gctggtgcgg gtgcagagca cgctcctgcc
       61 atccggggcg acccggaaac cggtgatgtg gcgaagatcg aggagctgac ggatcgcccc
      121 cttttgcagg tcgacctgat aaagaggaac atggccgcgc acatcggcgg tgaaatagag
      181 gctgcggctg tcggaggccc actgaaaagc gttgacccaa taatcgaacg gctcggtgag
      241 gatgcgatga atcccgtttt ggcggtcata aatggccaga cgtttgcggt ccgcttcata
      301 gaccggcacc ttctgcgtaa tgtaggcgat atagcggccg tcgggcgacc agaccggctc
      361 gccatcatag gcggtattat cctccgtgag gttgaggctg tcccctttga cgagggattt
      421 gatccagaca tccttgtcgg tggtctcgta atcgttgcgg tccggatgga gggcgatggc
      481 gagctcccgg ccgtccgggg agagggaaaa accggcatgg ccgccggcgc cggcatcggc
      541 gggctggtcg atcgcgcccg gagtgaggtc ggtgagctgg ctgtcggcca gggtgtattt
      601 aagggtgtgg atgacgcggc cgtcccgcca ccaggtccag tggcggtaga agaggcggtc
      661 ggcgaggtgg gcctgcaccg gcccctcctc catggtctcg agaatcttct tgctgcagtc
      721 atcgtcgccg ccgcactcgg gatagaccat ggtggtgaaa ataaagccgc cgccgtcagg
      781 cagccactcc ggatctcccg gggagacggc cagatcagtg aggcggcgcg cctcgccccc
      841 gtcggccggg agcagccaga tctgcccccc ctggtcgcgg ccggagagga aggcgatggt
      901 tttaccgtcc ggcgaccagc gcggatggta atcgccctgc gggctgaagg tgagctgccg
      961 caatccgctg ccgtcgaggt tcatcagcca gaggtcgctg ttgcgctccg acttggcgag
     1021 atcctggctg ccgacgacaa agcacactct tttgctgtcc ggcgagatct ggatgtcact
     1081 gatcgatttg agggcgtaga gatcggcgat ccggaaggcg cgtttctcgg ctgcgaacag
     1141 ggcgacgggg agtaacagca ggattaagaa tacaatcttt ttcatcggcg aatcctcatc
     1201 tggatcggag tggttttgcc tgaagccgcc atgcccgcgg ctggtgcgcc ggccgcacac
     1261 agacgcgtcc gggttaggtc cggcgaaaag ttgttatccc ttgtaccagt cccgcttcgg
     1321 ttcatagctg gtgcgcagcg tccggcggcg ctcctgccgg cgcagggcca gctcgatcag
     1381 ctcatcgagc agggcgctgt aggggagccc cgaggcctcc cacatcttgg gatacatgct
     1441 gatgcgggtg aagccgggga tggtgttgag ttcgctgaga taaaagcgtc cggtttcctt
     1501 ttccatgagg aaatcggcgc gggccatgcc ctcgcactcg gtggcgacat aggcgcggat
     1561 ggcggtctcc tgcagggcgc gcagctgccc gggggtgagc cgggcgggaa tctccaggcc
     1621 gctgttgtcg tcgacgtact tggcgtcgta atcgtagaat tcattgccgg gaaagacctc
     1681 accggcgacc gagacgcgcg gattctcgtt gccgaggatg ctgagctcga gttcgcgcgg
     1741 ctgggctaca gctttttcga ccagcacctt gcggtcatac tgcagggcct cctcgatggc
     1801 gttgaagagt tcgcggcgat cgtgggcctt gctgatgcca aggctggagc cgagattggg
     1861 cggcttgacg aaaaggggca ggccgagggc gtcgatgagc gagccgacaa tctcctcgcg
     1921 cgagcggttg gcgagctggt cgggcagggc cgggaagggt tcgctgcccc cctccgcgcg
     1981 ccagtcgatg tcgcggaacc agaggaaatc gatggtgggg atgccctgct ggcggcagat
     2041 gtgcttctgg acgaccttgt ccatggccat ggccgagccg agcactccgg cgccgacgta
     2101 ggggatatcc atgagctcga gcagtccctg gagggtgccg tcctcgccga aggggccgtg
     2161 cagggcggga aagaccacat ccagcttttc aaccggcagc aggccggagg cgcccttttg
     2221 catcagcagc tgatggctgc cggcgtcggg cgacagcagt gcgggcaggc cggggcgatg
     2281 gttcttgagc tctgcaagcg cattttcacc cgcaacccac tccccctgct tggtgatgcc
     2341 gatgggaatg atctcgtact tgtttttgtc cagcgcctcg atgatcgagg tggcggtgac
     2401 cagggagacc tcatgttcac ccgaccggcc gccaaagatg acccccactc gcagtttggc
     2461 cacgctctac ctccttgata agtcattgta aattattcca aaagggtgaa aaaagcaagg
     2521 gaat
//
LOCUS       DAOPWU010000446         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_43636_length_2511_cov_4.303746, whole genome shotgun sequence.
ACCESSION   DAOPWU010000446 DAOPWU010000000
VERSION     DAOPWU010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43636_length_2511_cov_4.303746"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..396
                     /locus_tag="PKM23_14920"
     CDS             61..396
                     /locus_tag="PKM23_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92814.1"
                     /translation="MQQTVMFVILGFLGFLVSTSALAQCLLKTIGMLHKKVKHPGFFA
                     GWSRQAADRPLATGIYYAVLTSSSAKRIIKMVLMQKAAGGFILGLANSRDAAVPSNYS
                     GLVSSGCSA"
     gene            complement(360..818)
                     /locus_tag="PKM23_14925"
     CDS             complement(360..818)
                     /locus_tag="PKM23_14925"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNY92815.1"
                     /translation="MRYFCQVQLERRHDYAVLLFAPAEDEAFTKKIAAMRKTQTGGHA
                     DETETAMMLSHRPELVHLDQANTQSGADQARLAELPFLFTGIWWYARFPNHYAGDGAP
                     AQAELGRLLIEHEVEQLVKMIASVKADAKVLPFQQEFYNQAEQPLETRPE"
     gene            complement(713..1249)
                     /locus_tag="PKM23_14930"
     CDS             complement(713..1249)
                     /locus_tag="PKM23_14930"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNY92816.1"
                     /translation="MIYPRMLPLLLLAVWLPAAGQKVPVLPVQYEELTSPQFVQAVKS
                     SQATCIIPIGIMEKHGPHLPLGTDLIDARKIALTAAAREYAVVFPPYYFGQINEAKHQ
                     PGTVAYSHELVWNILQETCEELARNGMKRSFWSTATAATTICCVIFVRCSWRDGMITR
                     YYFLRRPRMRPLQKRSPR"
     gene            complement(1323..1631)
                     /locus_tag="PKM23_14935"
     CDS             complement(1323..1631)
                     /locus_tag="PKM23_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNY92817.1"
                     /translation="MSSIFQSIHKLVAKIPPGRVATYGQIAAMLGVPGGARTVGWAMH
                     GLPEGSEVPWHRVVGAGGLVRLPEFEGKAVQVILLRQEDVEVDGRGRIDLERFQWTAE
                     "
     gene            1715..1891
                     /locus_tag="PKM23_14940"
     CDS             1715..1891
                     /locus_tag="PKM23_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92818.1"
                     /translation="MSASSQKTGGTMFMIAGALFLIIAIFGIKIIFFTLGLSLLLIGA
                     IVLSKAKSSPKNKG"
     gene            1969..>2511
                     /locus_tag="PKM23_14945"
     CDS             1969..>2511
                     /locus_tag="PKM23_14945"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNY92819.1"
                     /translation="MQQPFLLGVNYWPRRKAMYWWSDFDAGEVREEFALIREIGLEVV
                     RLFLTWEDWQPVPDVVDPGALNHFEEVCEIAAGLGLRLNVTFFTGHMSGPSWTPSWML
                     HPGQPMPPRIWQVVSGGRTVDCGYRNPFTDPLVLRAEELQLRTVVSRFRQHPAIEVWN
                     LGNEPDIFAWPADAAGGKEWT"
BASE COUNT          505 a          750 c          692 g          564 t
ORIGIN      
        1 tcggtttgac ctactagtgc ctgcggcgcg gccgcggact tttatttgga tgggggcctt
       61 atgcagcaaa cagtaatgtt cgtcatcctt ggctttctcg gcttcctggt cagcacatcc
      121 gccctcgccc agtgtctgct caagaccatc gggatgctgc acaagaaggt gaagcacccc
      181 ggattttttg ccggctggtc aaggcaagca gcggatcgtc ctctcgccac cggcatttat
      241 tacgcggtgc tgacgtcttc ttcggctaaa aggatcatca agatggtgtt gatgcaaaag
      301 gctgccggcg gttttatcct ggggcttgcc aattcacgag atgcggccgt accctcaaac
      361 tattccggcc tggtctcgag cggttgctcc gcctgattat aaaattcttg ctgaaacgga
      421 aggacttttg catcagcctt gacactggcg atcattttaa ccagctgttc cacctcgtgc
      481 tcgatgagca gcctgccaag ttcggcctgc gcaggagcgc catcgccggc ataatgattg
      541 ggaaaacggg cataccacca gatgccggtg aagaggaaag gcagctccgc caaacgcgcc
      601 tgatcggcgc cggattgcgt gttggcctga tccagatgca ccagctccgg ccggtgcgag
      661 agcatcatgg ccgtctctgt ctcatcagca tgcccaccgg tctgcgtttt cctcatcgcg
      721 gcgatctttt ttgtaaaggc ctcatcctcg gccggcgcaa aaagtaatac cgcgtaatca
      781 tgccgtctct ccagctgcac ctgacaaaaa taacgcagca gatagttgtt gctgccgtgg
      841 ccgttgacca gaatgatctt ttcatgccgt tgcgcgccaa ttcctcacag gtttcctgca
      901 ggatattcca gacgagttca tgactgtagg ccaccgtgcc gggctgatgt ttggcctcgt
      961 tgatctggcc aaagtaatag gggggaaaaa caacggcata ttcgcgggcc gctgccgtca
     1021 gggcaatctt tctcgcatcg atgagatcgg ttccgagcgg cagatgcgga ccgtgttttt
     1081 ccatgatacc gatcggaatg atgcaggtgg cttgactgga tttgaccgcc tgcacgaact
     1141 gcggcgaggt aagctcctca tattgcaccg ggagaaccgg cactttttgc cccgctgccg
     1201 ggagccacac cgccaatagc agtaatggta acattctggg atagatcaca gatacctcct
     1261 ttagctggtt tctccgacac agggttaatt agccgttgcc cgagcgctgc ggatcctctc
     1321 tttcattccg ctgtccattg aaatctctcc agatcgatcc gaccgcggcc gtccacttcg
     1381 acgtcctcct gacgcagcag tatcacttgc accgctttgc cttcgaactc gggcagacgg
     1441 acgagtcccc cggcaccgac cacacggtgc cagggcacct cgctgccctc cggcaagcca
     1501 tgcatcgccc agccgacggt gcgtgcgccg cccggcaccc ccagcatcgc cgctatctgc
     1561 ccataggtgg ccactctgcc cggaggaatt ttcgccacca gcttgtgtat gctttgaaaa
     1621 atgctcgaca tgtgttaaat ttaattaact aatgatcaga tgcaaggata atatctgtaa
     1681 cggaagtttc tcacaagaaa tcgggagtca acccatgtcc gcttccagtc aaaaaaccgg
     1741 cggtacaatg ttcatgatcg ccggagctct tttcctgatt atcgccattt tcgggatcaa
     1801 aataatcttt ttcacccttg gcctgagctt gctccttatc ggcgccattg ttctcagtaa
     1861 agcgaagagc agcccaaaga ataagggata aacgaagtca atccgcaggc gaggagtgcc
     1921 atctcgcctg cgccccatgg tagtagaaaa acagagaggg cgtgagccat gcagcagccg
     1981 tttcttttgg gtgtcaacta ctggccgcgg cgcaaggcca tgtactggtg gtcggatttc
     2041 gatgccgggg aggtccggga agaatttgcc ctcatccgcg agatcggcct cgaggtggtg
     2101 cgcctttttc ttacatggga ggactggcag ccggtccccg atgtcgtcga tcccggggcg
     2161 ctcaaccatt tcgaagaggt ctgcgaaatc gccgccggac tgggtctgcg cctcaatgtg
     2221 acctttttca ccggccacat gagcggtccc tcctggaccc cctcctggat gctgcacccg
     2281 ggccagccga tgccgccccg catctggcag gtggtcagcg gcgggcgcac cgtcgactgc
     2341 ggctaccgca accccttcac cgacccgctg gtcctgcgcg cagaggaact gcagctgcgc
     2401 accgtggtct cacgcttccg gcagcacccg gccatcgagg tctggaatct tggcaatgag
     2461 ccggacatct tcgcctggcc ggccgatgcc gccgggggga aggagtggac g
//
LOCUS       DAOPWU010000447         2508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_43719_length_2508_cov_3.759071, whole genome shotgun sequence.
ACCESSION   DAOPWU010000447 DAOPWU010000000
VERSION     DAOPWU010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43719_length_2508_cov_3.759071"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2052
                     /locus_tag="PKM23_14950"
     CDS             <1..2052
                     /locus_tag="PKM23_14950"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNY92820.1"
                     /translation="NLDETDRQVVIDRQPGQYLGHPTTVLLEDSTTMLTVYPKGHGSG
                     EIVYKRSFDGGVTWSRALPTPASWATSREVPTLFRMTDPGGVERLILFSGLYPARRAW
                     SADNGQSWSELEMAGDWGGIVVMGGAIRLKDGRYMALFHDDGRFFKGANKTNGLFIVF
                     KTLSSDGGLTWSHPEPIIECDWADPCEPGIFRSPDGQQILVLLRENRRKFNSFYITSD
                     DEGESWSPMVELPAALTGDRHTGQYTRDGRLFVSFRDMAKGSPTWGDWVGWLGTWEDI
                     LRGREGEYRILLKDNTYSADTAYPGVERLPSGMMVVTTYGHWATGEQPYIVSTRFMPE
                     ELGRSHPPAPVGPLKIVVAGDGAAGGGMEEGQPLKLYADHLAADLPAAGVAAEVLNRG
                     AAGLTSDAALQGFQQQVLDEKPDLVILHFGSEDAAVEVGATPARLVPRVPLERFTANL
                     TAMVQQLKARGIRPLLMTSHPLCWGDSSLARFGKPMPGSPYLVDDPFGLNTVLSRYTA
                     AVREVAIREEVQLVDIYGRFISHANVKGQELTDLVPDGLHANAAGQRRMAAAIYEVLG
                     IDVPEEPSGVEGGMESGSSGEEEAQPHKFGIGGYPNPFNPATELVVELPEPGPIRVEV
                     FDRQGRRVKLLAEGFFGKGVHRMAWDARDAEELPVAAGVYWARLSAGAHRDAHKMVVL
                     R"
     gene            complement(2056..>2508)
                     /locus_tag="PKM23_14955"
     CDS             complement(2056..>2508)
                     /locus_tag="PKM23_14955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY92821.1"
                     /translation="ETTDTLILTGVLKHFKVVRDLCKESSIVYGDRNLLEQLIRNLEI
                     NAAHAMKMGGKLEVRTRLSEDQSDCVLTIGDDGCGIPEEALEKIFQPFYTTKSEGQGT
                     GLGLPIVKDIVEKHNGTIRIESQVDVGTRVHIGLPVARGVTFTQGLAI"
BASE COUNT          435 a          791 c          838 g          444 t
ORIGIN      
        1 aatctcgacg agaccgaccg ccaggtggtc atcgaccgtc agcccgggca gtacctgggc
       61 catcccacca cggtgctgct cgaggacagc accaccatgt tgacggttta tcccaaaggc
      121 catggcagcg gagagattgt ctacaagcgc agcttcgacg gcggggtgac ctggagcagg
      181 gccctgccga cgccggcgag ctgggccacc tcccgggaag tgccgaccct gttccgtatg
      241 accgatccag ggggcgtgga gcgcctgatc ctcttctccg gtctctatcc ggcgcggcgc
      301 gcctggtcgg cggacaacgg ccagagctgg agcgagctgg agatggccgg ggattggggg
      361 ggcattgtcg tgatgggcgg cgccatccgc ctcaaggatg ggcgttatat ggccctcttt
      421 cacgatgacg gccgtttttt caagggtgcc aacaagacca acggcctctt catcgtcttc
      481 aaaaccctct cctcagacgg cgggctgacc tggagtcatc ccgagcccat cattgaatgc
      541 gactgggcgg atccctgtga gccgggcatc ttccgctcgc cggacgggca gcagattctg
      601 gtacttctgc gcgaaaaccg ccgcaagttc aactctttct atatcaccag cgatgacgag
      661 ggcgaaagct ggtcgccgat ggtcgagctg cccgccgccc tcaccggcga ccgccatacc
      721 gggcagtaca cccgcgacgg ccgtctcttc gtctccttcc gcgacatggc caagggcagc
      781 ccgacctggg gcgactgggt cggctggctc ggcacctggg aggacattct ccggggacgc
      841 gagggggagt accgcatcct gctcaaggac aacacctatt cggccgacac cgcctatccc
      901 ggggtggaac ggcttccctc gggcatgatg gtggtcacca cttacggaca ctgggccacg
      961 ggcgagcagc cctatatcgt cagcacccgt ttcatgccgg aggaactcgg gcggagtcat
     1021 ccccctgcgc cggtgggacc cctgaagatc gtcgtcgcgg gtgacggggc cgccggcggg
     1081 ggcatggaag aggggcagcc gctcaaactc tatgccgatc acctcgccgc ggacctgccg
     1141 gcggcgggcg tcgcagccga ggtgctcaat cgcggagccg ccggactcac cagcgatgcc
     1201 gccctgcagg gttttcagca gcaggtcctg gatgaaaagc cggatctggt catcctccat
     1261 ttcggcagcg aggatgccgc tgtggaggtg ggcgccaccc ccgcccgcct tgtcccgcgc
     1321 gtgccgctgg aacgtttcac tgccaatctc accgccatgg tgcagcagct caaggccagg
     1381 gggatccgcc cgctgctcat gaccagccac cccctctgct ggggcgattc cagtctggcc
     1441 cgtttcggca agcccatgcc gggctcaccc tatctggtcg atgatccatt cggcctcaac
     1501 accgtgctga gccgctacac agcggcggtg cgcgaggtcg cgatccgtga ggaggtgcag
     1561 ctggtggata tctacggccg cttcatcagc catgccaacg tcaagggaca ggagctgacc
     1621 gatctggtgc cggacggcct gcacgccaac gcggccggcc agcgccgcat ggccgcagcg
     1681 atctatgagg tgctggggat cgacgtgccg gaagagccga gcggcgttga ggggggcatg
     1741 gagagcggca gcagcgggga ggaggaggcc caacctcaca aattcggcat cggcggttat
     1801 cccaatccct tcaatccggc gaccgagctg gtcgtcgagc tgccggagcc ggggccgatc
     1861 agggtggagg tttttgaccg gcagggccgg cgggtcaagc tgctggcgga gggttttttc
     1921 ggcaaggggg tgcacagaat ggcatgggat gcgcgggatg cggaggaact gccggtggcg
     1981 gcgggggtat actgggcgcg gctctccgcc ggcgcccacc gggatgcgca caaaatggtg
     2041 gtgctgcgtt agggatcaga tcgccaggcc ctgggtgaaa gtgacacccc gggcgacggg
     2101 cagaccgatg tggaccctgg tcccgacatc cacctggctt tcgatgcgga tcgtcccgtt
     2161 gtgcttctcg acaatatcct tcacgatagg cagtccgagg ccggtgccct gtccctcgga
     2221 tttggtggtg taaaaaggct gaaagatctt ttccagggcc tcttcgggga tgccgcagcc
     2281 gtcatccccg atggtgagca cgcaatcgga ctggtcttcc gacaaacggg tgcgcacctc
     2341 cagctttccg cccatcttca tggcatgagc ggcattgatc tccagattac ggatgagctg
     2401 ctcgagaaga ttgcggtcgc cgtagacgat gctgctctcc ttgcagaggt cgcgcaccac
     2461 cttgaaatgc ttgagcacac cggtcaggat cagggtgtcg gtggtctc
//
LOCUS       DAOPWU010000448         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_43742_length_2507_cov_4.134992, whole genome shotgun sequence.
ACCESSION   DAOPWU010000448 DAOPWU010000000
VERSION     DAOPWU010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43742_length_2507_cov_4.134992"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKM23_14960"
     CDS             complement(<1..788)
                     /locus_tag="PKM23_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNY92822.1"
                     /translation="MRALDWIVLIAFLAFIALYGLWRGRHSNTASNYLLAGRSLRWYT
                     VLFSIMATQASAITFLSTPGQAYADGMRFIQFYLGLPLAMVVLSITVVPLFHRLKVYT
                     AYEYLEQRFDGRVRTLAAALFLIQRGLAAGLTIYAPALIFATILGWDIFWMNLVFGGV
                     VVFYTASGGTRAVSHTQTVQVLIIFTGMAVACAMLLHLLPAQVSLLDATRLAGKLGKL
                     ESIDPSFNLHSRYTLWSGLIGGFFLQLSYFGTDQSQVQRYLTGKS"
     gene            complement(788..955)
                     /locus_tag="PKM23_14965"
     CDS             complement(788..955)
                     /locus_tag="PKM23_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92823.1"
                     /translation="MSVQLKDPHHEPDQVDNAGGNKPLLFRAWRSWYILVIAELALII
                     LLLAWITGVFH"
     gene            complement(952..>2507)
                     /locus_tag="PKM23_14970"
     CDS             complement(952..>2507)
                     /locus_tag="PKM23_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92824.1"
                     /translation="PAAPAAALPPLLRAWEQLQKLADGPYVSQKRKELIAAIRACSGL
                     WLEAAADRPVAAAGDTLRLALQVVNRSPASWTLEKITFSWGHGDTLLQTALADNQPFE
                     KNTTLILPATLPVTQPFWLQQPAGRGLYAITEENLIGEPESPPSGTVTFHLRWRGQVI
                     PMTIPLLYRWTDPVEGDLYRPFVIGPPVAVHSRENLLLFTDDASRPLRLQLRSLTGAV
                     SGEVSLALPAGWSCTPQSIPFRLTARDENLELEFQLRPGKGALDGSVTALAGIGGRSW
                     SQDLITISYPHIPIQTLFPPAQVRLLRLPLPAAKPRIGYIMGAGDEIGDCLTAAGYPL
                     RMLTDDDLEKGNFAGLEVIIAGVRAYNTRPALAAAQPRLLRFVEAGGTLIVQYNTPFR
                     LVTGQLGPWPLRLSRDRVSDEQAPVTLLQPDHPLLTRPFRITPADFSDWVQERGLNFP
                     DQWDERYQTLIASHDPGEKDKSSGMLYTRCGKGIYIHSSYAWFRQLPAGVPGAWRLFI
                     NMIQAGAAP"
BASE COUNT          457 a          701 c          942 g          407 t
ORIGIN      
        1 gacttgccgg tgagatagcg ctgcacctgg gactggtcgg tgccaaagta ggagagctgc
       61 aggaagaatc ctccgatcag ccctgaccag agggtgtagc ggctgtgcag attgaaagag
      121 ggatcgatgc tctcgagctt gccgagcttg ccggccagcc gggttgcgtc gagcaggctg
      181 acctgcgcgg gcaggaggtg gaggagcatg gcgcaggcca cggccatgcc cgtgaagatg
      241 atcagcacct gcacagtctg ggtatggctg accgcgcggg tgccgccgct ggcggtataa
      301 aacacgacca cgccgccgaa gaccaggttc atccagaaga tatcccagcc gaggatggtg
      361 gcgaagatga gggcgggtgc atagatggtc agcccggcgg ccagccccct ctggatgaga
      421 aagagggcgg cggcgagggt gcggacacgg ccgtcgaagc gctgctcgag gtactcgtag
      481 gcggtgtaga ccttgaggcg atgaaagaga ggcaccacag tgattgagag cacgaccatg
      541 gccaggggca ggccgagata gaactggatg aagcgcatgc cgtcggcgta ggcctggccc
      601 ggcgtcgaaa gaaaggtgat ggcgctggcc tgggtggcca tgatcgaaaa gagcacggtg
      661 taccagcgca ggctgcggcc ggcgagaaga tagttgctcg cggtattgct gtgccttccc
      721 cgccacagcc catagagggc gatgaaggcg agaaaggcga tgaggacgat ccagtcgagg
      781 gcgcgcatca gtgaaagacc cccgtgatcc aggccagcag gaggatgatc agcgccagtt
      841 cggcgatgac gaggatgtac cagctccgcc atgccctgaa aaggagcggt ttgttcccac
      901 cggcgttatc cacctggtcg ggctcgtggt gtggatcttt caactggacg ctcacggggc
      961 tgctccggcc tggatcatgt tgatgaagag ccgccaggcg cccggcacgc cggccggaag
     1021 ctgccggaac caggcgtagc tgctgtggat atagatcccc ttgccgcagc gggtgtagag
     1081 catgccgctg cttttatcct tttccccggg atcgtgcgag gcgataaggg tctggtagcg
     1141 ttcgtcccac tggtccggga agttgaggcc gcgctcctgc acccagtccg aaaaatcggc
     1201 gggcgtgatc cggaaggggc gggtgagcag gggatggtcc ggctgcagca gggtcaccgg
     1261 ggcctgctca tcgctgacgc ggtcgcgcga gagccgcagc ggccagggac cgagctggcc
     1321 ggtcaccaga cgaaaggggg tattgtactg gacgatcagg gtgccgcccg cctcgacgaa
     1381 ccggagcagc cggggctgag cggcggccag ggcgggtcgg gtgttgtaag cgcgcacccc
     1441 ggcgatgatc acctccaggc cggcgaaatt gcccttttcc aggtcgtcgt cggtgagcat
     1501 gcgcaggggg taacccgcgg ccgttagaca atcgccgatc tcgtcccctg cccccatgat
     1561 atagccgatg cggggtttgg ccgccggcag gggcaggcgc agcagcctga cctgcgccgg
     1621 gggaaagagg gtctggatcg ggatgtgggg atagctgatg gtgatcagat cctggctcca
     1681 gctccggccg ccaattcccg cgagggcggt gacgctgccg tccagtgcgc cctttccggg
     1741 gcggagctgg aattccagct cgaggttttc atccctcgcg gtcagccgga aggggatgct
     1801 ttgaggtgtg cagctccagc ctgcgggcag tgcaagactc acctcgccgc tcacggcgcc
     1861 ggtcagactg cgcagctgga ggcggagcgg ccgggaggcg tcatcggtga agaggagcag
     1921 attctccctg ctgtggacgg ccaccggcgg cccgatgaca aaggggcggt agagatcgcc
     1981 ctcgaccgga tcggtccagc ggtagagcag gggaatggtc atgggaatga cctgaccgcg
     2041 ccaacgcaga tggaaagtga cggtgccgga cggaggggac tcgggttcgc cgatgaggtt
     2101 ttcttccgtg atggcataga ggccgcggcc ggccggctgc tgcagccaga agggctgggt
     2161 gacgggcagg gtggcgggca ggatgagggt ggtatttttc tcgaagggct ggttgtccgc
     2221 cagggcggtc tgcagcaggg tgtcgccgtg gccccaggag aaggtgatct tttccagggt
     2281 ccaggaggcg gggctgcgat tgaccacctg gagcgcgagg cggagcgtat cgccggcggc
     2341 agcgaccggc cggtcggctg ccgcttccag ccagagtccg ctgcaggcgc ggatggctgc
     2401 aatgagttct ttccgctttt gactcacata ggggccatcc gcaagttttt gcagctgttc
     2461 ccaggcgcgc agcaggggcg gcagggccgc agccggagcg gccggag
//
LOCUS       DAOPWU010000449         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_44697_length_2471_cov_2.997517, whole genome shotgun sequence.
ACCESSION   DAOPWU010000449 DAOPWU010000000
VERSION     DAOPWU010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44697_length_2471_cov_2.997517"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..60)
                     /locus_tag="PKM23_14975"
     rRNA            complement(<1..60)
                     /locus_tag="PKM23_14975"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(404..1690)
                     /gene="tyrS"
                     /locus_tag="PKM23_14980"
     CDS             complement(404..1690)
                     /gene="tyrS"
                     /locus_tag="PKM23_14980"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNY92825.1"
                     /translation="MNHGEAYQELSRRGFIQQVTEPEPLQELLAKGPVTFYVGIDPTA
                     DSLHVGHLMAVMGMMQMQKFGHRPIAIVGGGTAMVGDPSGKSEMRKMLTREAIAANGE
                     KIKIQLSRYLDFGAGKAIMVDNYDWLGELKYIEFLRDIGRHFSVNRMLTYEAYKIRME
                     KGLSFLEFNYQLLQAYDFLMLYDRHGCRLQMGGDDQWANILAGTDLIRRVRSVDAYGL
                     TFPLLTTATGAKMGKTESGAVWLDPDKTTPHEYFQYWVNSDDRDVARFLAFFTPLPMA
                     EIEPVSRLEGAELNAAKVVLAFEATALTHGLEAAQSAQAAAAAAFGGREIPAGLLPSS
                     RVGRGSVARDAAAIPASGIGRAALAAGVKLADLLVEHGLLSSKGEVKRLIQGGGLYLG
                     EERVEDPGRVVTEADAREGAIVVRLGKKRYHRFVLD"
     gene            complement(1711..2181)
                     /gene="smpB"
                     /locus_tag="PKM23_14985"
     CDS             complement(1711..2181)
                     /gene="smpB"
                     /locus_tag="PKM23_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNY92826.1"
                     /translation="MAKKEKSLQQMVNRRAYHDYEIVEKFEAGIALQGTEVKSVRAGK
                     VSLKDSYARVIDGELWMVNLHINEYDHGTIWNHDPARNRKLLLHRREIKRLTGKTEEQ
                     GMTLVPLSLYFKNGKVKVELGLGRGRKSYDKRAEIAKRDVNRDIERELKERQRN"
     gene            complement(2192..>2471)
                     /locus_tag="PKM23_14990"
     CDS             complement(2192..>2471)
                     /locus_tag="PKM23_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92827.1"
                     /translation="GLRMGSPPEGWMKNSVVAAAEHTLCGEGGCQRQFPAGDQVNIMP
                     MRLMLQVKKIICCLCSTATGIMGKRRKVGARFQGVDWFCLLVFMLKNT"
BASE COUNT          448 a          791 c          657 g          575 t
ORIGIN      
        1 ttgcatgtgt taggcacgcc gccagcgttc gtcctgagcc aggatcaaac tcttcattgt
       61 aatatatttc attttcaatt aacgagatta atctggcctt gaattgacga tagagccttg
      121 cacgctattt tcaaagaaca atttcgctgc tatttcggtg tttcgttcgc agccgtataa
      181 agtacgaaac ttttatcttc cagtcaagta ctttcttgct tttttagaac ttttttttgc
      241 gggctcgttt ttggcaaatt tgccgcacaa aatgggacat aatataaggc tttttatcga
      301 gaatgcaagc tttttttgcc aaaacagcct tttttccgct ctttttttgg gaaccaagcg
      361 ggctcctccc gcctccgcaa gcccagagcc gccaccattc cccttaatcc agaacgaaac
      421 ggtgataacg cttcttgccc agccgcacca cgatcgcccc ctcccgggca tcggcctccg
      481 tcaccacccg gccgggatcc tccacccgct cctcgcccag atagagtccg cccccctgga
      541 tcagccgctt cacttctccc ttcgaggaga gcagcccgtg ctccaccagc aggtcggcca
      601 gcttcacccc ggccgccagc gccgcccggc cgatcccgct cgccgggatg gcggcggcat
      661 cgcgcgccac actgccccgg cccaccctgc tcgagggcag cagcccggcg gggatctcac
      721 ggccgccaaa agccgcagcc gcagcagcct gcgcggactg cgccgcctcc aggccgtggg
      781 tcagcgccgt ggcctcgaag gcgagcacca ccttggccgc attcagctcc gccccctcca
      841 gccggctcac cggttcgatc tccgccatgg gcagcggcgt gaagaaggcg agaaagcggg
      901 cgacatcgcg gtcgtcgctg ttgacccagt actgaaaata ttcatgcggc gtggtcttgt
      961 ccgggtcgag ccagaccgcc cccgactcgg tcttgcccat cttggcgccg gtcgccgtgg
     1021 tcagcagcgg gaaggtgagg ccgtaggcgt cgacactccg cacccggcgg atcaggtccg
     1081 ttcccgccag gatgttggcc cactgatcgt cgccgcccat ttgcagccgg cagccgtggc
     1141 ggtcataaag catcaaaaaa tcataggcct gcagcagctg gtagttgaac tcgagaaagg
     1201 agagtccctt ttccatgcgg atcttgtagg cctcataggt gagcatgcgg ttgacgctga
     1261 agtggcggcc gatgtcgcgc aaaaattcga tatatttgag ttcgcccagc cagtcgtaat
     1321 tgtcgaccat gatcgccttg cccgcaccga aatcgagata gcggctgagc tgtattttga
     1381 tcttttcgcc gttggcagcg atcgcttcgc gcgtgagcat cttgcgcatc tcgctcttgc
     1441 cgctcggatc cccgaccatg gccgtgccgc cgccgacgat ggcgatgggg cggtggccga
     1501 acttttgcat ctgcatcatc cccatcaccg ccatgagatg gccaacgtgg aggctgtcgg
     1561 cggtggggtc gatgccgaca taaaaagtca ccggcccttt ggcgagcagc tcctgcaggg
     1621 gttccggctc ggtgacctgc tggatgaatc cgcgccggct cagttcctga tatgcttctc
     1681 cgtggttcac aaattgttct ccgctctggt tcagttccgc tgccgctctt tcagttcgcg
     1741 ttcgatgtcg cggttgacgt cacgcttggc gatttcagcc cttttgtcgt aactcttgcg
     1801 cccgcgcccg agccccagct ccaccttgac cttgccgttc ttgaaataga ggctgagggg
     1861 gaccagggtc atcccctgct cctcggtctt gccggtcagc cgcttgatct cgcggcggtg
     1921 gagcagcagc ttgcggttgc gcgccgggtc gtggttccag atcgtgccgt ggtcatactc
     1981 gttgatgtgg aggttgacca tccacagctc gccgtcgatg actcgggcgt agctgtcctt
     2041 caagctcacc ttgccggcgc ggaccgattt gacctcggtg ccctgcaggg cgatcccggc
     2101 ctcgaatttc tcgacgatct catagtcgtg ataggcccgg cggttgacca tctgctggag
     2161 gcttttttct ttttttgcca tagactatat attaggtatt tttcagcata aaaacaagca
     2221 aacaaaacca gtctaccccc tgaaatctgg ctcccacctt gcgtcgcttg cccataatcc
     2281 ctgtcgctgt actgcataaa cagcagataa tttttttcac ttgtaacatt agccgcatcg
     2341 gcattatatt cacctggtcg cccgcaggga attgacgctg gcatccgccc tccccgcaca
     2401 gagtatgctc agccgcggcc acaaccgaat ttttcatcca accctccggc ggagagccca
     2461 tgcgcaatcc c
//
LOCUS       DAOPWU010000450         2469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_44743_length_2469_cov_4.932891, whole genome shotgun sequence.
ACCESSION   DAOPWU010000450 DAOPWU010000000
VERSION     DAOPWU010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44743_length_2469_cov_4.932891"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1031
                     /locus_tag="PKM23_14995"
     CDS             156..1031
                     /locus_tag="PKM23_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92828.1"
                     /translation="MSLISRWKGLNHAARIVTGLAALVILLEFLFDIVEITIGHIMLW
                     TNDKRPKIGRLWIEEERDQTGRQQASTHIDSVRLQPAYERYIHSLDDLAAYVAFKSGF
                     DLSREEFLALYRSLPGQDAGRLVEPEILSGLAEAGSWASVKLERREDKLQLNFFDPYG
                     QPLHTTQIVMLSAAELMQDSRLEDNPVYAGRTIPAPLFVTALESLPQNLRLQVINDPQ
                     KWQEWRQRLIAAAVAPRIVHGSVTIALEIVQAGGVSVQEMEASELAVEYLINALNELS
                     PDTHYSSPNKEDEHE"
     gene            1024..>2469
                     /locus_tag="PKM23_15000"
     CDS             1024..>2469
                     /locus_tag="PKM23_15000"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNY92829.1"
                     /translation="MNKRSRRILWGVLLLSFILLLVAIYFSMLTCPRVPDDLNDIALS
                     NPSVIYGCEDEVVKLLADRVVISIDEMPPAVQQAFISLEDDSFYRHHGISKLHLMRAL
                     AENLVHLGGKGGGSSITQQLAKNLFFSFERDWARKFKEMFVAFQIEQQFTKEEILEAY
                     INQINFGSGVYGIELASQTYFAKHASELTLAESAILAGIPRWPARYNPSTNPGVAKER
                     QSFVLRRMVDEGFITEPERTVAWAESIKVDRVNRLQGSADYFVEEIKSRTADKLTPDA
                     VNFGGLRIYATVDSRLQLEATRAVAEGLARLDESLGLDPYNKASWEERINYPQAALVA
                     VDVRTGAVRAMVGGRDFRRAPFNRATANNRHAGSAFKPFIYFTALEKKLITPATVKVD
                     EEVAFPSGSNTWSPDNIDFNYLGPVTMKYALLRSINTISAKLIYELSPEAVTAAAQRL
                     GITSMLEPNLSLALGAAGVSPLEMASAFATIA"
BASE COUNT          490 a          798 c          650 g          531 t
ORIGIN      
        1 tctgctgctg gccatcttcg gcaggcggca tccctcgcct ctggatgatt atacccgcct
       61 ggatcaccgc cgccgcatta tcggcgcctt ggtcttgatc ctctttgtgc tctctttcac
      121 ccccgtcccc tttaaaattt agccggagct gccgaatgtc gctcatcagc cgctggaagg
      181 gactcaacca cgccgccagg atcgtcaccg ggcttgcggc cttggtcatc ttgcttgaat
      241 ttttatttga tattgtcgag ataaccattg gacatatcat gctttggacg aacgataaac
      301 ggccaaagat cggccggctc tggatcgagg aggagcgcga ccagactggt cgtcagcagg
      361 cctccaccca tattgattcc gtccgtcttc agcccgccta cgaacgctat atccactccc
      421 tggatgatct ggctgcctat gtggccttca agtcgggatt tgacctcagc cgcgaggagt
      481 ttctcgccct ctatcgctct cttccgggcc aggatgccgg tcggctggtg gaaccggaga
      541 tcctctccgg gctggcggaa gcgggttcat gggccagcgt caaactggaa aggagggaag
      601 acaagcttca gctgaatttt tttgatccct atggccagcc tcttcacacc acccagatcg
      661 tgatgctctc cgcggctgaa ctcatgcagg attcccgtct cgaggacaac cctgtctatg
      721 cgggccgaac catccccgcg cccctttttg tcaccgcact ggagagcctg ccccagaatc
      781 tgcgcctgca ggtgatcaat gacccgcaga agtggcaaga gtggcgccaa aggctgatcg
      841 ctgcagcagt ggcgcctcgc attgttcacg gctccgtgac cattgctctc gaaatcgttc
      901 aggctggcgg cgtctccgtg caggagatgg aggccagcga acttgccgtg gagtacctca
      961 ttaacgccct caacgaactc tctcccgata ctcattacag cagccccaac aaggaggacg
     1021 agcatgaata agagatcccg ccgcatcctc tggggcgttc tattattgtc gttcatcctc
     1081 cttctggtcg cgatctattt ctcaatgctc acttgtccgc gcgttcctga tgacctcaac
     1141 gacatcgccc tcagcaatcc ctccgtcatc tacggctgtg aggatgaagt ggtcaagctg
     1201 cttgctgacc gggtcgtgat ctcaattgat gaaatgccgc cggcggtcca gcaggccttc
     1261 atctctcttg aagacgacag cttttatcgt caccatggca ttagcaagct gcatctgatg
     1321 cgggctctcg ctgaaaatct cgtccatctg gggggaaagg gcggaggcag ctcgatcacc
     1381 cagcagctgg ccaaaaacct ttttttctcc tttgagcgcg attgggcccg gaaattcaag
     1441 gagatgttcg tcgcctttca gatcgaacag cagttcacca aggaggagat cctcgaggcc
     1501 tacatcaatc agatcaattt cggctccggc gtctacggca ttgagctggc cagccagacc
     1561 tatttcgcca aacacgccag cgagctcacc ctcgccgaat cggccatact cgccggcatt
     1621 ccgcgttggc cggcgcgcta caaccccagc accaatcccg gcgtggccaa ggagcggcag
     1681 tccttcgtgc tgcgccgcat ggtcgatgag ggctttatca ctgaaccgga acggacggtc
     1741 gcatgggccg aatcgattaa agtcgaccgc gtcaaccgcc tgcagggcag cgctgactat
     1801 ttcgtcgagg agattaaaag ccgcacggcc gacaagctca cgcccgatgc agtcaatttc
     1861 ggcggactgc gcatctacgc aaccgtagat tcccgtctgc agctcgaagc gacccgtgca
     1921 gtcgcagaag gactcgctcg tctggacgag tctctgggac tggatcccta taacaaggcc
     1981 tcctgggaag agcggatcaa ctatccccag gccgccctgg tcgccgtcga tgtccgcacc
     2041 ggcgccgtca gggcgatggt ggggggaagg gacttccgcc gcgcaccatt taaccgcgcc
     2101 accgccaata accgccacgc cggctccgct ttcaaaccct tcatctattt tactgccctg
     2161 gagaaaaagc tgatcactcc cgcgaccgtc aaggtcgatg aggaggtcgc cttcccgagc
     2221 ggcagcaata cctggtcgcc ggacaatatc gattttaatt acctcgggcc ggtgaccatg
     2281 aaatatgccc tgctgcgctc catcaatacc atctccgcca agctcatcta tgaactctcc
     2341 cccgaggccg tcaccgctgc cgcccaacgt ctgggcatca ccagcatgct ggagcccaac
     2401 ctttccctcg ctctcggcgc cgccggggtc tctcccctcg agatggccag cgccttcgcg
     2461 accatcgcc
//
LOCUS       DAOPWU010000451         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_44768_length_2468_cov_4.563199, whole genome shotgun sequence.
ACCESSION   DAOPWU010000451 DAOPWU010000000
VERSION     DAOPWU010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44768_length_2468_cov_4.563199"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKM23_15005"
     CDS             complement(<1..466)
                     /locus_tag="PKM23_15005"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HNY92830.1"
                     /translation="MLFSARLLAVDLVSPDGRLALDFYLDASGAPCYRLTLDSQPVIL
                     ESRMGITIRDNPGLFDGFKVLGFERSTTDESWEPVWGEERRIRSHYNELEVTLERVEP
                     EKRRMIIRFRLFDDGLGFRYEFPRQPGLGYFIVSGEKTQFNLTGDHLAWWIPG"
     gene            complement(569..1414)
                     /locus_tag="PKM23_15010"
     CDS             complement(569..1414)
                     /locus_tag="PKM23_15010"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNY92831.1"
                     /translation="MRRSMIALTATLLTALLAISDPVQAQFNMPRPSPKASVTQTIGL
                     TDVSISYSRPGVKNRTIWGELVPFGKVWRLGANEATIITLGDDVTIEGQPLARGSYSL
                     HAIPTSEEWTIIFNKVAEQWGSYNYKQEEDALRVTVKPEAGPFVERMLFTFDDITDES
                     ALVTLAWEKLRVSFKVGVNTQGKVINSAKATLSPRALFFAAQYAYQNNVELDQAARWL
                     DASIALEENYQNLSFKAGLLAKAGNRKEAVKLGEKALALGKAAQRIPPDLAAFEKQLL
                     EWKKR"
     gene            complement(1505..>2468)
                     /locus_tag="PKM23_15015"
     CDS             complement(1505..>2468)
                     /locus_tag="PKM23_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HNY92832.1"
                     /translation="PSGDMAGGPMYVLERGLGWRWMGILFAVFTAVAAFGIGCMVQAN
                     SISALALSAFQIPGWVTGLLLAILTAVVILGGIKSIARVCGVLVPFMALTYILGCLYI
                     LVLHYQTLPATIALIVRSAFNGHAAVGGFAGATLMAALRFGVARGLFSNESGLGSAPI
                     VAAAAQTRNPVRQALVSSTGTFWDTVVVCLMTGLVLVNSGDWTGGANGAELTKSAFSH
                     IPVIGPAVLTIGLLTFVFSTILGWSYYGEKAMEYLAGERAVLPYRILWCIAVYFGSVF
                     SLQFVWNFADLANGLMAIPNLIALLALSGVLVRETRTYLWDGEL"
BASE COUNT          515 a          714 c          803 g          436 t
ORIGIN      
        1 ccccggggat ccaccaggcg agatgatcgc cggtgagatt gaactgggtt ttttcgccgc
       61 tgacgatgaa atagcccagg ccgggctggc gcgggaattc atagcgaaag ccgagaccgt
      121 cgtcgaaaag gcggaaacgg atgatcatcc gccgcttctc cggctcaacg cgctccaggg
      181 tcacctccag ctcgttgtag tggctgcgga tgcgccgctc ttcgccccag accggctccc
      241 agctctcgtc ggtggtggag cgttcgaaac cgagcacctt gaagccgtca aagagtccgg
      301 gattgtccct gatggtgatg cccatcctgc tctccaggat gaccggctgg ctgtcgaggg
      361 tgaggcggta gcagggcgcg cccgacgcgt cgagataaaa gtcgagcgcc agccggccgt
      421 caggcgagac cagatcgacg gccaaaagcc gggcagagaa cagcatcagc ataaggaatc
      481 cgcttttttt catctccgca agacctccgg gttaatccgc aaggccaggc aaagcagctt
      541 cgcctggcct gcgggtggag catgttgctt atctcttctt ccactccagc aactgcttct
      601 caaaagcagc cagatccgga gggatgcgtt gtgcggcctt gccgagtgca agggccttct
      661 cgcccagttt gaccgcctct ttccggttgc cggctttggc gagcagcccc gccttgaagc
      721 tcaggttctg atagttctct tccagggcga tggaagcatc cagccacctg gcagcctgat
      781 cgagctcgac attgttctga taggcatact gagccgcgaa aaaaagagcg cgcgggctca
      841 gggtggcctt ggctgaattg atcaccttgc cttgcgtgtt cacacccacc ttgaaggaga
      901 cgcgcagctt ctcccacgcc agcgtgacca gggctgactc gtcggtgata tcatcaaagg
      961 tgaagagcat gcgctcgacg aagggaccgg cctcgggttt gactgtcact cgcagggcat
     1021 cctcttcctg cttgtagtta taggagcccc actgttccgc aaccttgttg aagatgatgg
     1081 tccactcttc ggaggtggga atggcatgca ggctgtagct ccccctggcc aggggttgac
     1141 cctcgatggt cacgtcatcg cccagggtga tgatcgtcgc ctcattggcg ccgagccgcc
     1201 agaccttgcc gaaaggcacc agttcccccc agatggtgcg gttcttgaca ccgggacggc
     1261 tgtaggagat cgatacatca gtcaggccga tggtctgggt gacgcttgcc ttggggctcg
     1321 gccgcggcat attgaactgc gcctgaacag gatcggagat ggccagcaga gctgtcaaca
     1381 gcgtggccgt cagtgctatc atgcttcttc tcattgctgc ctccatgatt aaggatacat
     1441 aactgcaggt cgacatcgct taaaagataa caccggattc cggataaaaa ttgcacaatg
     1501 caatctacaa ctcgccatcc cagagatagg tccgcgtctc gcgcaccagc accccgctca
     1561 gggcgaggag ggcgatgaga ttggggatgg ccatgagtcc gttggcgaga tcggcgaaat
     1621 tccagacgaa ctggagggaa aagaccgagc caaaatagac ggcgatgcac cagagaatgc
     1681 ggtagggcag caccgcccgt tcgccggcga ggtattccat cgccttttcg ccgtaatagg
     1741 accaaccgag gatggtcgaa aaaacgaagg tcaacagtcc gatggtgagg acggccggtc
     1801 cgatgaccgg gatgtgggag aaggcggatt tggtcagttc ggcgccgttg gcgcccccgg
     1861 tccagtctcc ggaattgacc agcaccaggc cggtcatgag gcagaccacg acggtatccc
     1921 agaaggtgcc ggtggaggag accagggcct ggcgcaccgg attgcgggtc tgggcggcgg
     1981 cggcgacgat cggggccgag cccaggcccg actcgttgga aaagaggccg cgcgcaaccc
     2041 cgaagcggag ggccgccatc agggtggctc cggcgaagcc gccgactgcg gcatggccgt
     2101 tgaaagccga gcggacgata agggcgatag tcgccggcag ggtctgatag tggaggacga
     2161 ggatgtagag gcagccgagg atgtaggtga gggccatgaa ggggacgagg acgccgcaga
     2221 cacgggcgat gctcttgatg cccccgagga tcaccaccgc ggtgaggatg gcgaggagaa
     2281 ggccggtcac ccagccgggg atctgaaagg cgctcagggc gagggcggag atcgagttgg
     2341 cctgcaccat gcagccgatg ccgaaggcag cgacggcggt gaagacggca aagaggatgc
     2401 ccatccagcg ccagcccagg ccgcgttcga gtacatacat cggcccccct gccatatccc
     2461 cgctcggg
//
LOCUS       DAOPWU010000452         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_44891_length_2463_cov_4.312708, whole genome shotgun sequence.
ACCESSION   DAOPWU010000452 DAOPWU010000000
VERSION     DAOPWU010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44891_length_2463_cov_4.312708"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKM23_15020"
     CDS             <1..333
                     /locus_tag="PKM23_15020"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNY92833.1"
                     /translation="GFLIKLFGGRTVVVEGVPSGMRIGAEDRILLSIIDQYREYAATE
                     TDMRERVAKSFACRSAIMAGERLSVEAMNALIDQLFATENPYFCPHGRPVMITITVDE
                     LDRRFDRK"
     gene            320..1279
                     /gene="miaA"
                     /locus_tag="PKM23_15025"
     CDS             320..1279
                     /gene="miaA"
                     /locus_tag="PKM23_15025"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNY92834.1"
                     /translation="MTANRVPILVGPTAAGKSAVALALAARLGGEIVSADSRQIYRHM
                     DIGTAKPSAAELDRVRHHFIGICNPDEEYSAGSYGRAARACIDGLFARGIQPVVAGGS
                     GFYIRALVDGLFAPQASDPAVKQKWRLRIAAEGAAAIHEELAKVDPASAARLHPNDIQ
                     RVIRALEVQEITGRPISDFQSGEEEPASFLPLFIGLVRPREALYRRIDLRVEQMLREG
                     LLEEIRRLTAMGYGLHNNALLTVGYQEILECPELLQPGADLSAAMLLIQQHTRQYAKR
                     QMTWFRRDERITWIDVEERSEEEIAGEIVNRWETYGRETERRD"
     gene            1298..1801
                     /locus_tag="PKM23_15030"
     CDS             1298..1801
                     /locus_tag="PKM23_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNY92835.1"
                     /translation="MSGKQRSEEKIVTEEQLKRRLQRERRAGRLIVWTNGVFDILHAG
                     HVTYLERAAALGDVLVVGLNTDASVQLNKGPLRPIVPERERARLLAALACVDYVILYG
                     DQSPVELIGRLKPDIYAKGGDYTVETINQPERRLMEGLGGRIELLPGVPGLSTTHIIE
                     RILKAYT"
BASE COUNT          474 a          837 c          767 g          385 t
ORIGIN      
        1 ggctttctga tcaagctctt cggcgggcgc accgtggtcg tcgagggggt gccctcgggc
       61 atgcgcatcg gggcggagga ccgcatcctg ctgagcatca tcgaccagta ccgcgagtat
      121 gccgccaccg agaccgacat gcgcgagcgg gtggccaaaa gcttcgcctg ccgctcggcg
      181 atcatggccg gcgagcggct ctcggtcgag gcgatgaacg ccctcatcga tcagctcttc
      241 gccaccgaga acccctattt ctgcccgcat ggcagaccgg tgatgatcac catcaccgtg
      301 gacgaactgg accgcagatt tgaccgcaaa tagagtccct atcctggtcg gcccgacggc
      361 ggccggcaag agcgccgtgg ccctggccct ggcggccagg ctcggcggcg agatcgtctc
      421 ggccgactcg cgccagatct accgccacat ggacatcggc accgccaagc cctccgcggc
      481 ggagctggat cgcgtccgcc accattttat cggcatctgc aaccccgatg aggagtacag
      541 cgccggcagc tacggccgcg ccgcgcgcgc gtgcatcgac ggcctcttcg cgcggggcat
      601 ccagccggta gtggccggcg gctcgggctt ttacatccgc gccctggtcg acggcctctt
      661 cgccccgcag gcctcggacc cggcggtcaa gcagaaatgg cgcctccgca tcgccgccga
      721 gggcgccgcg gccatccatg aagagctggc gaaggtggat ccggccagcg cggcgcggct
      781 ccatcccaac gacatccagc gcgtcatccg cgccctcgag gtccaggaga tcaccggccg
      841 gcccatctcc gactttcaga gcggcgagga ggagccggca tcgttcctcc ccctcttcat
      901 cggactggtc cgtccgcgcg aggctctcta ccggcgcatc gacctccgtg tcgagcagat
      961 gctcagggag ggactgctgg aggagatccg gcggctgacg gcgatgggat acggcctgca
     1021 caacaacgcc ctgctcacgg tgggttatca ggagatcctc gagtgcccgg agctgctcca
     1081 gccgggcgcg gacctctcgg cggcgatgct gctcatccag cagcacaccc gccagtacgc
     1141 caagcggcag atgacctggt tccgccgtga cgagcgcatc acctggatcg acgtcgagga
     1201 gcggagcgaa gaggagatcg caggagagat cgtcaaccgg tgggaaacat acggccggga
     1261 aacagagcgc agggattaat caacaggcgg gaaaaccatg agcgggaaac agcgcagcga
     1321 ggaaaagatc gtcaccgagg agcagctcaa gcggcggctg cagcgggagc gtcgggcggg
     1381 ccggctcatc gtctggacca acggggtgtt cgacatcctg cacgccggcc acgtcaccta
     1441 tctggagcgt gccgctgcgc tcggcgatgt gctggtggtc ggcctcaaca ccgacgcctc
     1501 ggtgcagctc aacaaggggc cgctgcggcc gatcgtgccg gagcgggagc gcgcccgcct
     1561 gctggcggcc ctcgcctgcg tcgattatgt gatcctctat ggcgaccagt cgccggtgga
     1621 actcatcggg cggctcaagc cggacatcta cgccaagggg ggggattaca ccgtcgagac
     1681 catcaaccag cccgagcggc ggctgatgga ggggttgggc ggccgcatcg agctgctgcc
     1741 cggcgtgccc gggctttcga ccacccatat catcgagcgc atcctcaagg cctacacctg
     1801 acccggagcg gagtaaagag cgctccggtt caagccggcc ggccaatggc cctcgcgcat
     1861 ccgcaacgaa aaagaacaaa aacaggcgat ccggtcgcca gccccaacat caagccgcca
     1921 catatggtgc acgccggcgc gcaaaagcgg caaaaatcgc tattttgaaa atatttcttg
     1981 acctgatatc aatttatgag tatcattcaa cacgcctgtg ggtgaaagag cactcagcag
     2041 cgcagagccg gcagggcatt tttccagctc gcccggccgc agcaccaggg gagggggtca
     2101 ctctctcttc tctccatatt cacctgcatc cgcaagaccg gaccagcaga cacgccgaat
     2161 cacaacggat tccacgctca aatcattcag tcagaggtta tccttcagca cgggaatacg
     2221 ccccggtaaa acaaacccgc atggccgtct gattttcagg ccgtcccggc cggctgcccc
     2281 ggccggccgc gcgcccgcac cgcaggcgcc atgaaggttt cttttatctt tccacctgtt
     2341 ggcctccccg ctgtccggta cacctcaccc agccttgatg aagcgccttt gccccgcgca
     2401 aggcgcgagc atggcatcaa tcgccccaaa agcgaattgc atcggcagcg cactcccatc
     2461 aag
//
LOCUS       DAOPWU010000453         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_45068_length_2457_cov_2.812656, whole genome shotgun sequence.
ACCESSION   DAOPWU010000453 DAOPWU010000000
VERSION     DAOPWU010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45068_length_2457_cov_2.812656"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PKM23_15035"
     CDS             <1..957
                     /locus_tag="PKM23_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92836.1"
                     /translation="FLAQRGDTVVGRCNAFVNHNHNQRWGDKVGFFGQFESIDDQEVT
                     NDLLDAAAAWLKSQGMTAMRGPQNLLVNEATPGLLVQGFETRPVIYYQYNKPCYEKLL
                     NDYGMKVVKRVKSWEVPVDRPMEEKLERVGKKVIEHYGVTIETWDQRPLAERKQEMLE
                     VYNDAWNDNWGYVPFTREEFFRNVDDMQLIMEKGLFLFVYVKGELAAFFGGVPNICER
                     LVPLSWCRRCELLRAGKMLLTRNRIKGFRLGYMGVKQKYRRLGLDGVMLWKQKIYSQQ
                     KGYEYCDFGWVLEDNVLVIRVGESMQDSRLSKVYAVMEKELA"
     gene            978..>2457
                     /locus_tag="PKM23_15040"
     CDS             978..>2457
                     /locus_tag="PKM23_15040"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNY92837.1"
                     /translation="MSYIPLHLHSNYSLCRGANTIEEICAAAEAHSFSHLALTDTNGL
                     YGLAFFLDLARQHHLQPLVGAHLESGEEEAVVLARSLEGYHSLCRLITAMHTRERPVL
                     AGLLQPPDAEIVILTPCIPLLSHLTAAGPRENLYAEVIPHAGRERLLHFARSREIPVA
                     ASCAAWFLGEEDYAVHRLLRAIGLNTTLEKIPAGEAASPEARLRSPREMEALFPDAPD
                     ALANTIAIARQCTFDLDFGRFIFPSFTGPGGEDARSWLRQEVLAGARWRYGEITPAVQ
                     SRMEFELKLINDKGFAPYFLVVADAVRQAPRTCGRGSAASSLVSYCLGITHVDPIRYD
                     LFFERFLNPGRSDPPDIDVDFPWDERDDVLNYLFRTWGQGCVAMIANHNSFKVRSAVR
                     EIAKVYGIPDSEIGAVTKKMSGYWQPASVEAMISSHPVFKGTELHPPWPEIIALAERI
                     RGFPRHLSLHCGGVVIAPDGLERYVPLQPAKKVLHLSGAIQDG"
BASE COUNT          450 a          854 c          696 g          457 t
ORIGIN      
        1 ttcctcgccc agcgcggcga caccgtggtc ggccgctgca acgcctttgt caatcataat
       61 cacaaccagc gctggggcga caaggtcggc tttttcggcc agttcgagag catcgacgac
      121 caggaggtga ccaacgacct cctcgatgcc gcagccgcct ggctgaaaag ccagggcatg
      181 accgccatgc gcggacccca gaatctgctc gtcaatgagg cgaccccagg cctgctcgtg
      241 cagggattcg aaacccgccc ggtcatctat taccagtaca acaaaccctg ctacgaaaaa
      301 ctgctgaacg actatggcat gaaggtggtc aagagggtta aatcctggga ggtcccggtt
      361 gaccggccta tggaggagaa actggaacgg gtcggcaaaa aggtgatcga acactacggt
      421 gtgaccatcg agacctggga ccagcgtccc ctggcggagc gcaagcagga gatgctcgag
      481 gtttacaacg acgcctggaa cgacaactgg ggctatgtcc ccttcacccg cgaggaattt
      541 ttccgcaacg tcgatgacat gcagctgatc atggaaaagg ggctcttcct cttcgtctat
      601 gtcaagggcg aactggccgc ctttttcggg ggggtgccca atatttgcga acgcctggta
      661 cccctcagct ggtgccgtcg ctgcgaattg ctccgggcgg gcaagatgct cctaacccgc
      721 aaccggatca agggattccg cctcggctac atgggcgtga agcaaaaata ccgccgcctt
      781 ggcctcgatg gggtcatgct ctggaaacag aagatttact cccagcagaa gggctacgaa
      841 tactgtgact tcggctgggt gctcgaggat aacgtgctgg tcatccgcgt cggcgagtcc
      901 atgcaggatt cccggctctc gaaggtctat gcggtcatgg aaaaggaact cgcctgaccg
      961 caggggcgct cttccctatg agctatattc cccttcacct gcattcaaac tattcactct
     1021 gccgcggcgc caacacgatt gaagagatct gcgccgcggc ggaggcgcac tccttctccc
     1081 atctcgccct gaccgatacc aacggcctct acggcctcgc ctttttcctc gatctggccc
     1141 gccagcacca cctgcagccc ctcgtcggcg cccatctcga gagcggggag gaggaggcgg
     1201 tcgttctcgc ccgcagcctg gagggctacc actccctctg ccgcctcatc acggccatgc
     1261 acacccggga gcggccggtt ctggccgggc ttctccagcc cccggatgcg gaaatcgtca
     1321 ttctcacccc ctgcatcccc ctcctcagcc atctcaccgc ggcggggccc cgggagaatc
     1381 tctacgctga ggtcatcccc cacgccggac gcgaacgcct gctccatttc gcccgcagcc
     1441 gcgagatccc ggtcgcagcc agctgcgccg cctggtttct cggtgaggag gactatgccg
     1501 tccaccgcct tctgcgcgcc atcggcctca ataccaccct cgaaaagatc cccgcaggcg
     1561 aggccgcctc tccggaggcc cgcctccgtt ccccccgcga gatggaggcg ctctttcccg
     1621 atgcccccga cgcccttgcc aacaccatcg ccatcgcacg ccagtgcaca ttcgatctcg
     1681 atttcggccg cttcattttt ccctccttca ccggacccgg tggcgaggac gcccggagct
     1741 ggctgcggca ggaggtcctg gccggggcgc gctggcgcta tggcgagatc actccggcgg
     1801 tgcagagccg catggagttt gagctcaagc tgatcaacga caagggtttt gccccctatt
     1861 tcctcgttgt cgccgatgcg gtccggcagg ccccccgcac ctgcggccgc ggctcggccg
     1921 cctccagcct ggtcagctac tgtctcggca tcacccacgt cgacccgatc cgctacgatc
     1981 tcttcttcga gcgctttctc aatcccggcc gcagcgatcc ccccgacatc gatgtcgatt
     2041 ttccctggga cgagcgggat gatgttctca actatctctt ccgcacctgg ggccagggct
     2101 gcgtcgccat gatcgccaac cataacagct tcaaggtgcg ctcggcggtg cgcgagatcg
     2161 ccaaggtcta tggcattccc gacagcgaaa tcggcgcggt caccaaaaag atgtccggct
     2221 actggcagcc cgccagtgtg gaggcgatga tcagctccca cccggtcttc aaagggacgg
     2281 aactgcaccc gccctggccg gaaatcatcg cgctggcgga gcgcatccgc ggctttcccc
     2341 gccatctctc cctccactgc ggcggcgtgg tgatcgcccc ggacggcttg gagcgctatg
     2401 tccccctcca gcccgccaaa aaagtcctgc atctctccgg cgccatccag gacggca
//
LOCUS       DAOPWU010000454         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_45139_length_2454_cov_5.222176, whole genome shotgun sequence.
ACCESSION   DAOPWU010000454 DAOPWU010000000
VERSION     DAOPWU010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45139_length_2454_cov_5.222176"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PKM23_15045"
     CDS             <1..1156
                     /locus_tag="PKM23_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY92838.1"
                     /translation="HYTLHAGYRPQRGMQVEMNANLANIDLSDPGLISAPARGQWYDL
                     RRRGLDLSLTDENRLGELNLKLHGNFGRHRVYDGFRSDDYTYGLMLYQSSSLWRGVTT
                     TLGVDWKSYGGKAENIIAGRDFGSHRISELAPYFHLQQLLLRRVILSLGLRAEHHGLY
                     GSVLLPKAGVVINPTESLSLRFSAAEGFRSPTIRELYLFPAPNSGLEPESMWNYEMAL
                     SWMTAERFRLETVLFRSEGSDLIRLQGRYPNMLLSNSGAFVHSGYEISASWLPFAEGE
                     ITASWSKLDLDDQTMNSPGKKLSLGIDLPWRSLRFSLAAQHIRDLHGADFGAKPLPDY
                     TLVDAGVQWRVLSWLSGRLLLKNALDAEYQTMTGYPMPRRYAMLEWTAGI"
     gene            1192..1800
                     /locus_tag="PKM23_15050"
     CDS             1192..1800
                     /locus_tag="PKM23_15050"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY92839.1"
                     /translation="MSHQHRLYFNALAQEWPSRREPETELEARLEGFDIGEGDWILDL
                     GAGKGRLAAALRSRCGTEGRIVALDCAERMLAAGADEYTAAGVEPLCCDAALLALRAE
                     SFDKAVCLGTWPHFCHPQRVMQELLRILRPGGGLLILHTCSSRELNRYHALLPGVVAA
                     DRLVSARTLARELAGCGFVSLLHEEGPRQYRVQAQKPGGEPS"
     gene            complement(1900..>2454)
                     /locus_tag="PKM23_15055"
     CDS             complement(1900..>2454)
                     /locus_tag="PKM23_15055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY92840.1"
                     /translation="GKGTIELSWTTQSETSNLGFHLYRSLSGDGPFSRITTALIPGAG
                     SSQSRRSYAYSDPLPSVEGTFYYRLSDISYAGVETMHGLASATLVTAPEEYGLAQNYP
                     NPFNSSTAINFIMKESGGVRIMIHNLLGQAVRLLIDEVREAGEHRLLWDGRDENGITV
                     PAGVYLYTMQAGQFRTTRKLQFLR"
BASE COUNT          427 a          785 c          770 g          472 t
ORIGIN      
        1 ccactatacc cttcacgccg gctatcgacc gcagcggggc atgcaggtgg agatgaatgc
       61 caacctggcc aacatcgacc tttctgatcc cggcctgatc tcggcgccgg cccgcggcca
      121 gtggtacgac ctccgccgcc gcgggctgga tctctcgctc actgacgaga accgcctggg
      181 cgagctcaat ctcaagctgc atggcaattt cggacgccat cgcgtttacg acggattccg
      241 ctccgacgat tatacctacg gcctcatgct ctaccaatcc agctcgctct ggcgaggggt
      301 caccaccacc ctgggggtgg actggaagag ctatggcgga aaagcggaga atatcatcgc
      361 gggacgggat ttcggcagcc acaggatcag cgagctggcc ccctatttcc accttcagca
      421 gctcctcctc aggcgcgtta ttctttccct cggcctgcgc gccgaacacc acggcctcta
      481 cggctcggtc ctcctgccca aggccggggt ggtgatcaat cccacggaga gcctgagtct
      541 gcgtttttcg gcagcggagg gtttccgcag cccgaccatc cgcgaactct atctcttccc
      601 ggcgcccaat tccgggctgg agcccgaatc gatgtggaat tacgagatgg ccctctcctg
      661 gatgacagcc gagagattcc gtctcgagac ggtgctcttc cgcagcgagg ggtcggatct
      721 gatccgcttg caggggcgct atcccaatat gcttctctcc aacagcggcg cctttgtcca
      781 cagcggctat gagatcagcg ccagctggct ccccttcgca gagggggaga taaccgcttc
      841 ctggagcaag ctggatctcg acgaccagac catgaacagc cccggcaaaa agctcagcct
      901 cgggattgac ctcccctggc ggagcctgcg cttcagcctg gcggcccagc atatccgcga
      961 cctgcacggc gccgatttcg gtgccaaacc cctgcccgat tatacactgg tcgatgcagg
     1021 ggtgcagtgg cgcgtgctct cctggctgag cggccggctg ctgctgaaaa atgcccttga
     1081 tgccgaatac cagaccatga ccggctatcc catgccgcgg cgctatgcca tgctggaatg
     1141 gacggcgggc atctgatcgc ctcttccggc cgctaaccag acggagtgcc tatgtcccat
     1201 cagcatcgcc tctatttcaa tgcgctggcg caggagtggc cgtcgcgcag agagcctgag
     1261 accgagctgg aggcgcggct ggaggggttc gatatcggag aaggggattg gattcttgat
     1321 ctgggggcgg gcaaggggcg gctggcggcc gcactacgca gccgctgcgg gacggaaggc
     1381 agaattgtcg ccctcgactg cgcggaaagg atgctcgcgg cgggggcgga tgagtatacc
     1441 gctgcgggag tggaacccct ctgctgcgac gccgccctgc tcgccttgcg agctgaatct
     1501 ttcgacaagg cggtctgcct gggaacctgg ccccactttt gccatccgca gagggtgatg
     1561 caggagctgc tccggatcct gcgcccgggg ggcggattgc tcatccttca tacctgctcc
     1621 agccgggagc tgaaccgcta ccatgccctt cttcccgggg tggttgccgc cgaccggctg
     1681 gtgagcgcgc gcacgctggc gcgcgaactt gccggctgcg gcttcgtcag cctcctccat
     1741 gaagaggggc cccggcagta ccgggtgcag gcgcaaaaac cgggaggcga accctcctga
     1801 tccgctctcg cattctttcc ggaccttcag tatcgggccg ctgcttcagg cccgcctccc
     1861 ggattcaccc gcattatcct ggtataaaaa aactttcgat caacgcagga actgcagttt
     1921 gcgcgtcgtc ctgaactggc ccgcctgcat ggtataaagg taaacccctg ccggcacggt
     1981 aatcccgttt tcatcccggc cgtcccagag caggcggtgt tcacccgctt cccgcacctc
     2041 atcaatgagc agccggaccg cctgcccgag caggttgtga atcatgatcc gcacccctcc
     2101 gctctccttc atgataaagt tgatcgcggt cgagctgttg aagggattgg gataattctg
     2161 cgcgaggccg tattcctccg gggctgtgac cagggtggcg gaggcgaggc cgtgcatggt
     2221 ctccacgccg gcatagctga tgtccgagag ccggtaataa aaggttccct ccaccgacgg
     2281 cagggggtcg ctgtaggcgt agctgcgtct ggactggctg ctgccggcgc ccgggatgag
     2341 ggcggtggtg atgcgggaga aggggccgtc cccggaaagg ctgcggtaga gatggaagcc
     2401 gaggttgctg gtttcgctct gggtggtcca ggagagctcg atggtgccct ttcc
//
LOCUS       DAOPWU010000455         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_45208_length_2452_cov_3.528577, whole genome shotgun sequence.
ACCESSION   DAOPWU010000455 DAOPWU010000000
VERSION     DAOPWU010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45208_length_2452_cov_3.528577"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PKM23_15060"
     CDS             <1..686
                     /locus_tag="PKM23_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNY92841.1"
                     /translation="SVSVMPCTAKKYESDRAEMSSSGFKDVDAVLTTREAATMMKQIG
                     IHLPDLPDEEYDAPLGISTGAAVIFGNTGGVMEAALRTVYEVVTGKTLEEVEIKAVRG
                     LEGVREAEVQVGDLTVRAAVANGLANARQLMKKIVAGEAHYHFIEIMACPGGCIGGGG
                     QPVPTSMEIRKKRAAAIYRADQAKTIRKSHENPAVQQLYKEFLGEPNSHKAHELLHTH
                     YIKKTVYQE"
     gene            746..1822
                     /gene="buk"
                     /locus_tag="PKM23_15065"
     CDS             746..1822
                     /gene="buk"
                     /locus_tag="PKM23_15065"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNY92842.1"
                     /translation="MTDSLSPLILVVNPGSTSTKVGLFTGKKKVSEENLVHSREELAR
                     YQSLWDQFDYRTDLVIEFLNRQNLKAFSLDAVVGRGGMLKPLSRGTYVVDETMIEDAR
                     RGVQGQHSSNMGCAIAQKIAQLYGCVAFIVDPVSVDEFDVLARYSGHPRIARRSLSHA
                     LSLRAAAIWAAESMKMDPNKRNFIVAHLGGGISIAPVKGGHIIDVNDASSDGPFSPER
                     TGSLPLQPFIDLCFSGEFTKKEVQQMVMGKGGLLAYLGTTDAREIERRIAAGDSTAGE
                     VFAAMAYQIAKEIAAMASVLCGEVAAIVLTGGLARSALLVDLISTRIKFIAPIKVIPG
                     ELEMEALAAGALRVLTGQEKARRY"
     gene            1856..>2452
                     /locus_tag="PKM23_15070"
     CDS             1856..>2452
                     /locus_tag="PKM23_15070"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNY92843.1"
                     /translation="MITSFDDLLDRMRQGPRVTVAVAAADDEAALGAVAAAHELGMAD
                     ALLFGDGARISAVYEEMTGSPDLPFEIREAAEEQAARAAVAAVRSGAAQILLKGKIKT
                     AALLKAVLDSEHGLRSSRQLSDLFIFEDPSRAGNKLVMITDGGVTLKPDLKQKIEIIE
                     NAVAVARALGNDSPRVAVLSAVETVNPALPATIDAAVLT"
BASE COUNT          503 a          784 c          728 g          437 t
ORIGIN      
        1 tttcggtctc ggtcatgccc tgcacagcga aaaagtacga gagcgaccgc gccgagatga
       61 gcagcagcgg cttcaaggat gtcgatgccg ttctcaccac cagagaagca gccacaatga
      121 tgaagcagat cggcatccat ctgcccgacc tgccggatga agagtatgat gcgccgctgg
      181 gaatttcgac cggcgccgcg gtcatcttcg gcaacaccgg tggcgtcatg gaggccgccc
      241 tgcgcacggt ttacgaggtg gtcaccggca agactctgga agaggtcgaa atcaaggctg
      301 tccgcggcct ggaaggcgtc cgcgaagcgg aggttcaggt cggcgatctc accgtgcggg
      361 ctgccgtcgc caacggcctc gccaacgccc gccagctcat gaaaaagatc gttgccggcg
      421 aggcccacta tcactttatc gagattatgg cctgccccgg cggctgcatc ggcggcggtg
      481 gtcagccggt tcccacttcg atggagatca ggaaaaaacg cgctgcagcc atctatcgtg
      541 ccgatcaggc caagaccatc cgcaaatcgc acgagaatcc ggccgtgcag cagctctata
      601 aggaattcct gggcgaaccc aacagccaca aggcccatga actccttcat acccattata
      661 tcaaaaaaac ggtgtatcag gaatagcacc agtgcactcc gcccggcgcc cttgctccgg
      721 gcggagtctt caggagggtg gcatcatgac ggatagcctt tcgcccctca ttttggtggt
      781 taatccgggc tccacctcga ccaaggtggg gctcttcacc ggcaaaaaaa aggtttcgga
      841 agagaatctc gtccattccc gggaggagct ggcgcgctac cagagcctct gggaccagtt
      901 cgattaccgc accgacctcg tcatcgaatt tctcaaccgg cagaacctca aggccttctc
      961 cctcgatgcg gtcgtcggca gagggggtat gctcaagccc ctgagccgcg gcacctatgt
     1021 cgtcgatgag accatgatcg aggatgccag aaggggggtt cagggccaac acagctcaaa
     1081 catgggttgc gccatcgccc aaaagatcgc ccaactctac ggctgtgttg cctttatcgt
     1141 cgatccggtc tcggtcgacg agttcgacgt cctcgcccgc tactccggcc acccgaggat
     1201 tgcacggcgc tccctgtcgc atgccctcag cctgcgcgca gccgcgatct gggctgcgga
     1261 atccatgaaa atggatccga acaagcgcaa ttttatcgtc gcccatctcg gcgggggcat
     1321 ctccattgcc cctgtcaagg gcggccacat cattgatgtc aacgacgcct ccagcgacgg
     1381 ccccttctcc ccggaacgga cgggaagcct gcccctgcaa cccttcattg acctctgctt
     1441 ctccggcgag ttcacaaaaa aggaggttca gcagatggtg atgggcaagg gcggattgct
     1501 cgcttacctg gggaccactg acgcgcgcga gattgaaaga cgcattgcgg ccggcgacag
     1561 caccgccggc gaggtttttg ccgccatggc ctatcaaatc gccaaggaga tcgccgccat
     1621 ggccagcgtg ctctgcggag aggtcgccgc catcgttctc accggcggac tcgcccgttc
     1681 agccctgctg gtggatttga tcagcacgcg catcaaattt atcgccccga tcaaggtcat
     1741 accaggcgaa ctcgaaatgg aagccctggc tgccggggca ttgcgcgtgc tcaccggtca
     1801 ggagaaagcc cggcgctact gaaccacatg cagataggcc aaacaggagt cccccatgat
     1861 tacctccttc gatgacctgc tcgacaggat gcggcagggt cccagggtga cagtggccgt
     1921 cgccgctgcc gatgacgagg ccgcactggg cgcggtggcg gccgcacacg aactgggcat
     1981 ggcggatgcc ctcctcttcg gcgatggcgc caggatcagc gccgtgtatg aagaaatgac
     2041 cggctccccc gatttgcctt tcgaaatccg tgaggccgcg gaggaacagg cagcccgggc
     2101 ggcggttgcg gcggtgcgca gcggcgcggc tcaaatcctg ctcaagggca agatcaagac
     2161 cgccgcgctg ctcaaggcgg tgctggacag tgaacacggg ctgcgctcca gccgccagct
     2221 cagcgacctc ttcatctttg aggatccctc gcgcgccggc aacaaattgg taatgatcac
     2281 cgacggcggt gtgaccctca agcccgatct gaagcagaaa atcgagatta tcgagaatgc
     2341 cgtcgcagtg gcccgcgccc tcggcaatga ctcgccgcgc gtggccgtgc tctctgccgt
     2401 ggagacagtc aatcccgccc tgccggccac tatcgatgcc gctgtcctga cc
//
LOCUS       DAOPWU010000456         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_45405_length_2445_cov_3.525523, whole genome shotgun sequence.
ACCESSION   DAOPWU010000456 DAOPWU010000000
VERSION     DAOPWU010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45405_length_2445_cov_3.525523"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PKM23_15075"
     CDS             <1..932
                     /locus_tag="PKM23_15075"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNY92844.1"
                     /translation="SPRIADTAKILWGVYLLLTLVEVVLLLFGGMSLFDSLCHTFGSM
                     ATGGFSTKNASVAHYNSLYIEMVILVFMFLAGTNFSLHFRFLTGKHDVYRLDREFRFY
                     FLFTLIAILIVSGIVFLSKPVGPGGALRSASFQVVSVMTTTGFCTDDYELWLPSGQLL
                     MILLMFVGGCAGSTGGSVKVIRIMVVFKHSLTEIKKLLHPNAYIPVRLGGRVLAPDVV
                     TNILAFLIIYMLVFVAGSVGMALLGVDVVTAFSSVAATLGNIGPGLNLVGPIDHYGHL
                     PAAGKWLLSFCMLAGRLELYTVFILFIPDFWKK"
     gene            complement(1115..>2445)
                     /locus_tag="PKM23_15080"
     CDS             complement(1115..>2445)
                     /locus_tag="PKM23_15080"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNY92845.1"
                     /translation="IAGIFVVRTKEGAGQKELLGALSRGVNFSSILIVVASFFVVQAL
                     GLGYGVFWAIVCGLLAGIIIGKATEYFTSAGFKPTQRIADSAKTGPATVIIAGIGTGM
                     MSTTIPVLAVVVGTVLAYLTAADFNFANVNMGLYGIGIAAVGMLSTLGITLATDAYGP
                     IADNAGGNAEMSGLDKEVRKRTDALDSLGNTTAATGKGFAIGSAALTALALLASYVEE
                     LKIGLIRLGQTTIQFLDGRVVETAKGSFVDFMYYFNVTLMNPIVLVGIFIGAMTAFMF
                     SGLTMTAVGRAAAKMVDEVRRQFREIAGIMEGKAEPDYARCVAISTKGAQRAMMLPSL
                     LAIFIPVATGLVLGVAGVMGLLIGGTSTGFVLAVFMANAGGAWDNAKKYIEEGHMGGK
                     GSEAHKAAVIGDTVGDPFKDTSGPSLNILIKLMSMVSIVVAGLTVAYSLM"
BASE COUNT          445 a          758 c          744 g          498 t
ORIGIN      
        1 tcagcccgcg catcgccgac accgcaaaaa ttctctgggg ggtctatctg ctgctcaccc
       61 tggtcgaggt tgtgctcctg ctcttcggcg gcatgtcgct gtttgactcc ctctgccaca
      121 ccttcggctc catggccacc ggcggctttt cgactaaaaa tgccagcgtc gcccactata
      181 acagcctcta tatcgaaatg gtcatcctgg tttttatgtt cctcgccggc accaatttca
      241 gcctccactt tcgcttcctc accggcaagc atgacgtcta ccgcctcgac cgtgaattcc
      301 gcttttattt ccttttcacc ctgatcgcca ttctcatcgt ttccggaata gtcttcctca
      361 gcaagccggt cggacccggc ggcgccttgc gctccgcctc ttttcaggtg gtctcggtca
      421 tgaccaccac cggattctgc accgacgatt acgaactgtg gctgccttca ggacagctgc
      481 tgatgatcct gctcatgttc gttggcggct gcgccggttc gaccggcggc tcggtcaagg
      541 tgatccgcat catggtggtc ttcaagcaca gcctcaccga aattaaaaag ctgctccatc
      601 ccaatgccta tatcccggtc cggctgggcg gacgcgtgct cgcgcccgat gtggtcacca
      661 acatcctcgc ctttctcatc atctatatgc tcgtcttcgt cgccggatcg gtcggcatgg
      721 ccctgctcgg cgtcgatgtc gtcaccgcct tttccagcgt cgccgccacc ctcggcaaca
      781 tcggccccgg cctgaacctg gtcggaccga tcgaccacta cggccatctg cccgcagccg
      841 gcaaatggct gctctcattc tgcatgctcg ccggccggct cgagctctat accgtcttca
      901 tcctcttcat ccccgatttc tggaaaaaat agccttcgtg tggagagggc ggactgcccg
      961 gaccacgggg atgccggcag ccgcgtctga actgataccg gccataaaaa aagggcgatt
     1021 caatcgaatc gccctttttt tatggattgt tttagcggcc cggcggatag caaccggccc
     1081 ggtggccggc tctctcccca ggtgcaggtt ggaattacat cagcgaatag gccaccgtca
     1141 gacccgccac cacgatcgag accatgctca tcagcttgat gaggatgttg aggctggggc
     1201 cggaggtgtc cttgaagggg tcgccgacgg tgtcgccgat gaccgcggcc ttgtgggctt
     1261 cggagccctt gccgcccatg tggccttctt cgatgtactt cttggcgttg tcccaggcgc
     1321 caccggcatt tgccataaag acggcgagga cgaaaccggt gctggtgccg ccgatgagca
     1381 gacccatgac accggctacg cccagaacca ggccggtagc gacgggaata aagatggcga
     1441 gcagcgaggg cagcatcatg gcgcgctggg cgcccttggt ggagatggcg acgcagcggg
     1501 catagtcagg ttcggccttg ccctccatga tgccggcgat ctcgcggaac tggcggcgca
     1561 cttcatcgac cattttcgca gcggcgcggc cgacggcggt catggtcagt ccggaaaaca
     1621 tgaaggcagt catggcgccg ataaagatgc cgacgagcac aatcggattc atcagggtga
     1681 cattgaaata gtacataaaa tcgacaaagg agcccttggc ggtctcgacc acccggccgt
     1741 cgaggaactg gatggtggtc tggccgaggc ggatgaggcc gatcttgagc tcctcgacgt
     1801 aggaggcgag cagggccaga gccgtgaggg ccgccgagcc gatggcgaag cccttgccgg
     1861 tggcggcggt ggtgttgccc agggagtcga gggcgtcggt gcgtttgcgc acctccttgt
     1921 cgagcccgct catctcggcg ttgccgccgg cgttgtctgc gatcgggccg taagcgtcag
     1981 tggccagggt gataccgagt gtggagagca tgccgaccgc ggcgatgcca atcccgtaaa
     2041 gccccatgtt gacgttggcg aagttgaaat cggcggcggt cagataggcc aggaccgtgc
     2101 cgacgacgac ggcgaggacg ggaatggtcg ttgacatcat gcccgtgccg atgccggcga
     2161 tgatcactgt ggcaggcccg gtcttggccg agtcggcgat gcgctgagtg ggcttgaagc
     2221 cggccgaggt gaaatattca gtcgccttgc cgatgataat gccggcgagg aggccgcaga
     2281 cgatggccca gaagaccccg tagccgaggc cgagcgcctg gacgacgaag aatgatgcaa
     2341 ccacgatcag gatcgagctg aaatttaccc cgcggctgag ggcgccgagg agttccttct
     2401 ggcccgcccc ttccttggtg cgcacgacaa aaatgccggc gatgg
//
LOCUS       DAOPWU010000457         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_45847_length_2427_cov_3.923272, whole genome shotgun sequence.
ACCESSION   DAOPWU010000457 DAOPWU010000000
VERSION     DAOPWU010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45847_length_2427_cov_3.923272"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="PKM23_15085"
     CDS             complement(<1..1732)
                     /locus_tag="PKM23_15085"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNY92846.1"
                     /translation="MQEQLQRRADELEVINSIAAMTNASLDLDTILDNAIKSLIRLTR
                     ADHGVLYLLDEERPGNMRPIRYRGMTKREAFELIPVDTPTSMSWAALRQRRVIHVPDI
                     HAVLAYPPLYGAGICSFVTIPLFSNERPIGSMNLGMRSAKPLQVYSIEALTSIGSQIG
                     VALEHARLLTSMEREIEERRKAEAELRASEVRYQQIILASPEAIAITDVKGKYLLVNE
                     LYARLFGYESREAFQHAGLWAKDILTPEFMARLQTIYHSLKIGETMSGLRGMMRRRDG
                     SEIHVEISAAILRDAQGLPIGMIGIVRDVTEMHRWEEALRRSEERYRTLAEAAHDQIF
                     IVDQNDQLVYVNQFASQFIAKSPSEMIGRPRSDFFGGSTGDRQRSHILKIFENGKPMY
                     EENRIDLPGRIHWLGTWLAPIFDREGKVIQVLGVARDITDYKETASRLAASEQRFRDT
                     IERSSDGYFFIDNNGFVTGWNKAWLRILRFSQEDVDNLHITSHTDENARILLERLFNR
                     VMSGDIIPAQEMQIPVKDGELRWISFNARRVMRQGIVLGIEGFLRDVTEQVATLESLR
                     TVSLRLVQVQE"
     gene            complement(1951..>2427)
                     /locus_tag="PKM23_15090"
     CDS             complement(1951..>2427)
                     /locus_tag="PKM23_15090"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92847.1"
                     /translation="REAGLTSGEWDKIAFVEGHHSSMSRKSYRYLDPVQKEGSYGYRL
                     KQIDLDGTWSYLPAREASWVQPGLFALEQNYPNPFNPVTTIRYRLAEAGAVSLVIFNS
                     RGQEVRRLVEGMQGPGEFQVKWDGCDAFGQPAAGGLYFYLLRCGDQLLRRSMALLR"
BASE COUNT          463 a          743 c          717 g          504 t
ORIGIN      
        1 cctcctgcac ctgcaccagc cgcagcgaga ccgtgcgcag gctctccagg gtggccacct
       61 gttcggtgac gtcgcgcaga aagccctcga tcccgaggac gatgccttgc ctcattaccc
      121 ggcgggcatt gaagctgatc cagcgcagtt ccccgtcctt gaccggaatc tgcatctcct
      181 gagcggggat gatatctccg ctcatcaccc gattgaacaa cctctcgagg agaatccgcg
      241 cgttttcatc tgtatggctg gtgatatgca gattgtcaac atcctcctgg ctgaaccgaa
      301 gaatgcgcag ccaggccttg ttccagccgg tgacaaaacc gttgttgtcg ataaaaaaat
      361 agccatcgga cgaacgctcg atggtgtcac ggaagcgctg ctccgaggca gcgagccggc
      421 tggcggtctc cttatagtca gtgatgtcgc gggcgacgcc gaggacctga ataaccttgc
      481 cctcacggtc gaaaatcggc gccagccagg tcccgagcca gtgaatgcgt ccgggcagat
      541 cgatccggtt ttcctcgtac atcggcttgc cgttctcaaa gatctttaga atatgacttc
      601 gctgccggtc accggtcgaa ccgccgaaaa aatcgctgcg cggccggccg atcatctcgc
      661 tgggcgattt ggcgatgaac tgcgaggcga actggttgac atagacgagc tgatcgttct
      721 ggtcgacgat gaagatctgg tcatgagcgg cttcggccag ggtgcggtag cgctcttcgc
      781 tcctgcgcag ggcctcctcc cagcggtgca tctctgtgac atcccggacg atgccgatca
      841 tgccgatagg cagaccctgg gcgtcccgca ggatggcagc gctgatctcg acgtgtattt
      901 cactgccgtc ccgacgccgc atcatcccgc gcaagccgct catggtctcc ccgatcttga
      961 gggaatggta gatcgtttgc agccgggcca taaattcggg ggtgagaata tcctttgccc
     1021 agagtccggc atgctggaag gcttcgcgcg actcgtaacc aaacagccgc gcgtagagtt
     1081 cattaacgag caggtacttg cccttgacgt ctgtgatcgc gatcgcctcg ggcgaagcaa
     1141 gaatgatctg ctggtagcgg acctcgctgg cgcgcagctc cgcctcggcc ttgcgcctct
     1201 cctcgatctc cctctccatg ctcgttaaaa gccgtgcatg ctcgagggcg acgccgatct
     1261 ggctgccaat gctggtcagg gcctcgatgg aataaacctg caggggcttg gcactgcgca
     1321 tgcccagatt catcgagccg atcggacgtt cattgctgaa aagggggatg gtgacaaaac
     1381 tgcagatgcc ggcgccgtag agggggggat aggctaggac ggcatggata tcggggacgt
     1441 ggatgacccg ccgctgacgc agcgccgccc aggacatgga ggtgggggta tccaccggta
     1501 tcagctcaaa ggcctcacgc ttggtcatgc cgcggtagcg gatcggccgc atgttccccg
     1561 gtcgttcctc atcgagaaga tagaggacgc catgatccgc ccgggtcagc cggatcaggc
     1621 tcttgatggc gttgtcgaga atggtatcca gatcgagcga ggcgttggtc atggcggcga
     1681 tgctgttgat cacttcgagt tcatccgccc ggcgctgcag ctgctcctgc atccgcttca
     1741 gctcagcgat ctcgcgggat tcagatgact gcacaccctg taagggtgtt gaacgaagcg
     1801 atgcccgctc gctttttatt gcctcccctg tctgggactt tctcttcttg ccgggcacct
     1861 tctcccgcct ccctgaatga tgaaatgcaa aggggttctt tttctgaatt tcatcatctt
     1921 ggagataaat tgcaagaaga attctgcgcc ctagcgcaac agggccatgg atcgccgtaa
     1981 aagctgatcg ccgcagcgca gcaggtagaa atagaggccg cccgccgccg gttggccgaa
     2041 ggcgtcacag ccgtcccatt tcacctgaaa ttcacccggc ccctgcatcc cctcgaccag
     2101 ccgccgcacc tcctggccgc ggctgttaaa gatcaccagc gagaccgccc ccgcctccgc
     2161 cagcctgtag cggatggtcg tgaccggatt gaagggattg ggatagttct gctcgagggc
     2221 aaaaagcccg ggctggaccc aactcgcctc ccgcgccggc aggtaactcc aggtgccgtc
     2281 aaggtcgatc tgtttgagcc ggtagccgta gctcccctcc ttctggacgg ggtcgagata
     2341 gcggtaactt ttccggctca tgctgctgtg atggccctcg acaaaagcga ttttatccca
     2401 ctcgccgctc gtgaggcccg cctcgcg
//
LOCUS       DAOPWU010000458         2422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_45981_length_2422_cov_4.431770, whole genome shotgun sequence.
ACCESSION   DAOPWU010000458 DAOPWU010000000
VERSION     DAOPWU010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45981_length_2422_cov_4.431770"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1482
                     /locus_tag="PKM23_15095"
     CDS             145..1482
                     /locus_tag="PKM23_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92848.1"
                     /translation="MKRINLWVVVILLGGAGSGLSQYYDGGMGYYGQEGGRFMGGLGF
                     ASISEGGETRNYLSVAFRPEFCFGKIGVGLNINLLYDVEGGGIRARDWDSSYDYFRLL
                     RYLRYGRKMDKTYARIGTLDAARLGHGFIVNYFTNEASYDNRKIGLELDLDFGEYGFE
                     SAVSNLGRSDLVGLRVYLRPMYTLDIPVLKNFAMGATYAADFDPDVNAGSDDGISVIG
                     GDLELPLVRSPVFTTMLYFDYAHIRGYSSVEHKSRIFGAGKALGLYAGISNLFGLAEV
                     SARIERRWLGKEFIASYFDPFYEIDRFRIVSGDTLHKADALLGVTEETRGVFGELYGG
                     LLGNKVRLLGMMSRLDSQPHSGKLHLAAEAPDLVPVLAAHMTYDKGYIEKVGDLFSLD
                     DRSVARLGLGYKLKPYLIVYIDYIWTYVETEEGSHIYRQQRRIEPKMVFSYNF"
     gene            1586..2287
                     /locus_tag="PKM23_15100"
     CDS             1586..2287
                     /locus_tag="PKM23_15100"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNY92849.1"
                     /translation="MAWTRLQRLGSLARPPVDFIYPPLCLLCDGYLQPAEELICRTCF
                     RHLPQLEDPDIPSLSLRTPLRQPLYFERAISLYDYTEAVGKLIHFFKYSGGRTLARPF
                     GEALGLALLDGGYTPAAIVPVPLHPARRRERGYNQSELLARAIGAVTGLPVEPEGLRR
                     VTATAPQARMGRQKRLRNLRGAFSAGPHFDPAGGEIVLVDDVLTTGHTLDECARVLVQ
                     QGGGRVIAATIARVR"
BASE COUNT          433 a          752 c          751 g          486 t
ORIGIN      
        1 ctcggtcgac aagtccctgc tgcagggagg gcaggcccat tttcaatggg cgacccattt
       61 tgacatgccg cccggcatgg aagacctctg gtaaggcggg ggatgcactt ttagctcatg
      121 catcatcggg aaaggcgcgg tcgtatgaag cgaatcaacc tgtgggtggt ggtgattctt
      181 ctcggggggg caggcagcgg gttatcccag tattacgatg gcggcatggg atactacggc
      241 caggaaggcg gccgctttat ggggggcctg ggtttcgcca gcatctccga gggcggggag
      301 accagaaact atctttcggt cgccttccgt ccggaatttt gctttggcaa gatcggtgtc
      361 ggcctgaaca tcaatctgct ctacgatgtt gagggcggcg gaatacgcgc cagggactgg
      421 gacagcagct acgactattt ccgtcttttg cgctacctcc gctacggccg caagatggac
      481 aagacctatg cccgcatcgg caccctcgat gccgcccgtc ttggccacgg ttttatcgtc
      541 aattatttca ccaatgaagc ctcgtacgac aatcgcaaga tcggcctgga gctggatctc
      601 gacttcggcg agtacgggtt tgaaagcgcc gtcagcaacc tcgggcgcag cgatctggtc
      661 ggactgcgcg tctacctgcg cccgatgtac accctcgaca tcccggtcct taaaaatttc
      721 gccatggggg ccacctatgc cgctgatttc gaccctgacg tcaatgccgg cagtgatgac
      781 ggcatctccg tcatcggcgg cgacctcgaa ctgcccctgg tccgctcccc ggtctttacg
      841 accatgctct acttcgatta tgcccatatc cgcggctaca gctcggtgga acacaaatcc
      901 cgcatcttcg gcgccggcaa ggcccttggc ctctatgccg ggatcagcaa cctcttcgga
      961 ctggcggaag tctctgcgcg catcgagcgc cgctggctgg gcaaggagtt catcgcctcc
     1021 tatttcgatc ccttttatga gatcgaccgc ttcaggatcg tctccggcga caccttgcac
     1081 aaggccgacg ccctgctcgg ggtcaccgag gagacccggg gcgttttcgg ggaactttac
     1141 ggcgggctgc tcggcaacaa ggtgcggctg ctcgggatga tgagccgcct cgattcccag
     1201 cctcacagcg gcaagctgca cctggcggcc gaagcccccg atctggtccc ggtgctggcc
     1261 gcccacatga cctatgacaa gggctatatc gaaaaggttg gggatctctt ctccctcgac
     1321 gaccgcagcg tggcgcgcct ggggctcggt tacaaactga aaccctatct catcgtctac
     1381 atcgattaca tctggaccta tgtggagacc gaagagggca gccatatcta ccggcagcag
     1441 cgccggatcg agcccaaaat ggtcttttcc tacaactttt aacctcccgc acgggaggcc
     1501 gcatgagcgg atgagccgga gcagccggct tgtccccggg gtggaggccg ggtgaacaag
     1561 agatggttga ttgcgggggt gctccatggc ctggacccgc ttgcagcggt tgggttctct
     1621 ggcccggccg ccggtcgatt ttatctatcc accgctctgc ctgctctgcg atggctacct
     1681 gcagcccgcg gaggagttga tctgccgcac ctgttttcgc catctgcccc agcttgaaga
     1741 tcccgacatc ccgagcctga gcctgcgcac cccgctgcgg cagcccctct atttcgagcg
     1801 cgccatctcc ctctacgact ataccgaagc ggtcggaaag ctcatccatt ttttcaaata
     1861 cagcggcggg aggaccctcg cccgcccctt cggagaggcc ctcgggctgg ccctgctgga
     1921 cggcggctat acgcctgcag cgattgtgcc ggtgcccctg catccagccc ggcggcgcga
     1981 acggggttat aatcagtccg aactgctggc ccgggcgata ggggcggtga ccggccttcc
     2041 ggtcgagccg gaggggctgc ggcgcgtgac cgctactgct ccgcaggcgc gcatgggacg
     2101 gcagaagcgg ctgcgcaatc tgcgcggggc cttctccgcc ggcccgcact tcgaccccgc
     2161 ggggggcgaa atcgtgctgg tcgatgatgt gctcaccacc gggcacaccc tcgatgagtg
     2221 cgcccgggtg ctggtccagc aggggggcgg gcgggtgatc gcagcaacca tcgcacgggt
     2281 gcgctgactc tggcggcaga tggagaggga aagggaggga gtgaaccgct gttttgctgc
     2341 gtcaggaggc gctaaagtca attttgccag gctacccccc gaaatctggt gccgacccga
     2401 gtgtaattat aggaaaataa tt
//
LOCUS       DAOPWU010000459         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_46082_length_2418_cov_4.911553, whole genome shotgun sequence.
ACCESSION   DAOPWU010000459 DAOPWU010000000
VERSION     DAOPWU010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46082_length_2418_cov_4.911553"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKM23_15105"
     CDS             <1..223
                     /locus_tag="PKM23_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92850.1"
                     /translation="GLAWYLQVVGLDIGDAMKASSMMVPAVVRTQISATDFWIGFIPG
                     LLSMTLGNALSGVRIYRRDTAQLFKELEA"
     gene            220..954
                     /locus_tag="PKM23_15110"
     CDS             220..954
                     /locus_tag="PKM23_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNY92851.1"
                     /translation="MKCTILCLILVLAAGSVPAQTAGEIIRKIDDNLSARNRVTESAM
                     TIHGRRGSRTLTAKSYAEGTRKAFTEYLSPAAERGTKMLKLEGQLWIYSPGTDRTVQI
                     SGHLLRQSVMGSDLSYEDMMDDRKLAEIYEPSIAGEEELDGRPVWILELTARVSDAAY
                     PRQKLWVDRERFVPLRQELYARSGKLLKTALLSDLQRVGGRWYPMQVLYKDMLKQGKG
                     TEFRTTQIAFDQAIPEWIFTKAALKQ"
     gene            complement(1141..>2418)
                     /locus_tag="PKM23_15115"
     CDS             complement(1141..>2418)
                     /locus_tag="PKM23_15115"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNY92852.1"
                     /translation="LWSRPGWGFAGMVTVDRFALAFQALFLLGGLLTVLISFNRIEEE
                     YVQHGDFFGLLLLALFGLMVMTSTTHFLVIFLGLEILSIALYVLAGYRKMRVDSLEAA
                     LKYLLLGAFATGFFLYGVALIYGATGQLEMAHIREAAAAGGAGWLLPAGALLVLIGFA
                     FKAALVPFHMWTPDVYQGAPTPVTAFMSTGTKAAAFAVLARLVSIAVPVETIAWGPVL
                     AVLAVLTMTVGNFMAIVQSNVKRMLAYSSIAHAGYLLVALAAGNAAGRSAILYYLLVY
                     TLMNIGAFGVIAWLGRSGREERLTFESYRGLGYRYPFVSLAMAAFMFSLAGIPPTGGF
                     LGKFFLFAAAVRAEYIPLVVIAVLNAVVSVFYYMRLVVNLYMREAEEPAEAGKAHPLL
                     IAALVIALIGVVWLGVAPGGVLGAFNAAATAGM"
BASE COUNT          522 a          786 c          752 g          358 t
ORIGIN      
        1 cggcctggcc tggtatctgc aggtggtggg cctcgatatc ggcgacgcca tgaaggcttc
       61 cagcatgatg gtgcccgcgg tggtgcgcac acaaatcagc gccaccgatt tctggatcgg
      121 cttcatcccc ggcctcctct ccatgaccct gggcaacgcc ctctccggcg tccgcattta
      181 cagacgcgac accgcgcagc ttttcaagga gctcgaagca tgaaatgtac tatcctctgc
      241 ctgatcctgg tcctggccgc cgggtctgtg cccgcccaga ccgccggcga gatcatcagg
      301 aaaatcgatg ataatctctc cgcccgcaac cgcgtgaccg agtcagccat gaccatccat
      361 ggccggcgcg gcagccgcac cctcacggcc aaaagctatg ccgagggcac tcgcaaggct
      421 ttcaccgagt acctctcccc agccgcggaa cggggcacca agatgctcaa actcgagggc
      481 cagctctgga tctactcccc gggcaccgac cgcactgtgc agatttccgg ccacctcctg
      541 cgccagtcgg ttatgggcag cgacctctcc tacgaggaca tgatggacga ccgcaaactc
      601 gccgagatct atgagccatc gatcgccggc gaggaggagc tcgacggccg cccggtctgg
      661 attctcgaac tcaccgcccg ggtctccgac gccgcctatc caaggcaaaa gctctgggtc
      721 gaccgcgagc gcttcgtccc cctgcggcag gagctctatg ccagaagcgg caagctgttg
      781 aagacggccc tgctcagcga tttacagagg gtggggggac gatggtatcc gatgcaggtg
      841 ctgtacaagg acatgctcaa gcagggcaag gggacggagt tccggaccac acaaatcgcc
      901 tttgatcagg cgatcccgga gtggattttt accaaggcgg ctctgaagca gtaagcgacg
      961 gtactgcccg ccggccctgg ggatcccggg cgggcccgct gcggccggcc tagtccacaa
     1021 agctgcgttc aggggagcgc ctgtgaaaac agaactcgca acaaaaaaag cccggccata
     1081 ctggccgggc tttccatttc cccgttgcgc gccctcccgg cgccgaaatc cctcgctgcg
     1141 ctacattccc gcagtcgccg ccgcattgaa agcccccagc actccccccg gcgccacacc
     1201 cagccatacc accccgatca gggcgatgac aagtgcagcg atgagaaggg gatgggcttt
     1261 tcccgcctcg gccggctcct ccgcctcgcg catgtagaga ttgactacca ggcgcatata
     1321 ataaaagact gaaaccacgg cgttcagcac ggcaatgacc acgaggggga tgtactccgc
     1381 cctgaccgca gcggcgaaaa ggaaaaattt gccaagaaaa cccccggtcg gggggatgcc
     1441 ggccaggctg aacataaagg ccgccatcgc gaggctgacg aaggggtagc ggtagccgag
     1501 gccgcgatag ctctcgaagg tgaggcgctc ctcgcgtccg ctgcgcccga gccaggcgat
     1561 caccccgaag gcgccgatgt tcatcagggt atagaccagc agatagtaga ggatggcgct
     1621 gcgcccggcg gcgttgccgg ccgccagggc gacgagcagg tagccggcgt gggcgatgct
     1681 cgagtaggcg agcatacgct tgacattcga ctggacgatg gccataaagt tgccgacggt
     1741 catggtcagc acagccagaa cggcaaggac gggcccccag gcgatggtct ccaccggcac
     1801 cgcgatggag acgagccgcg ccagcacggc aaaggccgcc gccttggtgc cggtggacat
     1861 gaaggcggtc accggtgtcg gcgccccctg gtagacgtcc ggggtccaca tgtgaaaggg
     1921 gaccagggcc gccttgaagg caaaaccgat cagcaccagc agggccccgg cgggtaaaag
     1981 ccagcccgcg ccgcccgccg cggcggcctc acggatatgg gccatctcga gctgaccggt
     2041 ggcgccgtaa atcagagcta ccccgtagag gaaaaatccg gtcgcgaagg cgccgagaag
     2101 gagatatttg agcgccgcct cgagcgagtc gacgcgcatc ttgcgatagc cggccagcac
     2161 ataaagggcg atggagagga tctccagccc gagaaaaatc accaaaaaat gggtggtcga
     2221 ggtcatgacc atcagtccga acagggccag aagcagcagt ccgaagaaat ccccatgctg
     2281 cacatactct tcctcgatac ggttgaatga gatcagcacc gtgagcagcc ccccgagcag
     2341 gaagagggcc tggaaggcga gggcgaagcg atccaccgtg accatgcctg cgaaacccca
     2401 gccgggccgg gaccagag
//
LOCUS       DAOPWU010000460         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_46113_length_2417_cov_4.870872, whole genome shotgun sequence.
ACCESSION   DAOPWU010000460 DAOPWU010000000
VERSION     DAOPWU010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46113_length_2417_cov_4.870872"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PKM23_15120"
     CDS             <1..712
                     /locus_tag="PKM23_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNY92853.1"
                     /translation="VGTTDTEVEEPLLEPRASAEEIEFILKTCEAYLDKDPRPADILS
                     VFAGLRPLAVPEEKSGPTKDVTRKHQIIISPSGLITVTGGKWTIYRKMAEDAIDKAVQ
                     VGCLPEKECVTKTLPVHGYLRNMDFDAPLSYYGSDRVVLEETVMAEEPALGERIHPAF
                     PYIRAEVVWAVRHEMARTVEDFLARRVRALFLDARSSIAAAPAVAEIMAAELGRDAAW
                     ARGQVEEYTELAKGYIVG"
     gene            complement(884..2191)
                     /gene="thrC"
                     /locus_tag="PKM23_15125"
     CDS             complement(884..2191)
                     /gene="thrC"
                     /locus_tag="PKM23_15125"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNY92854.1"
                     /translation="MKFASRLHPHDQATFRQALFSGIAPDGGLYHPVDHPDLSALIAA
                     FDESVTFNDVAALVSAALLAPEIDEAAARRIAGRAFSFAPRLVRIDGGRLLLELFHGP
                     TCAFKDFGARFLAASMEELLEGERCQILVATSGDTGSAVANAFHNRANIDVILLYPSG
                     RVSALQEQQLTTLGGNVHAIEVRGSFDDCQRLVKAAFRDRRLRAALRLTSANSINIGR
                     LLPQSFYFIHAAAQRRVLSGAPPRFCVPSGNFGNLTAGLYAWHWGMEAAGFLAATNIN
                     DVVPDYLKSGVYTPRPSLRTLSNAMDVGHPGNFERMEQLFDGNWRGMKAVVGSGVVDD
                     ERTLWSMHQLSSRFGMLVDPHTAVGCAVAEDFIEGSKRGGEQVVVLATAHPGKFAETV
                     KKATGHEPELPERLARCLALPKQALAMGPELAELSTFLLERFA"
     gene            complement(2188..>2417)
                     /locus_tag="PKM23_15130"
     CDS             complement(2188..>2417)
                     /locus_tag="PKM23_15130"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92855.1"
                     /translation="LAAGALGCSISGSGPSLFALCADLAAAARAGEAMKSAFEQAGLA
                     ADLFLSRVNPDGPAVLDEETSPNQPASGENA"
BASE COUNT          433 a          714 c          866 g          404 t
ORIGIN      
        1 cgtcggcacc accgataccg aggtggagga gccgctgctc gagccgcggg cctcggccga
       61 ggagatcgag ttcatcctca agacctgcga ggcctatctc gacaaggacc cgcggcccgc
      121 ggatatcctc agcgtcttcg ccggactgcg ccccctggcc gtgcccgagg agaagagcgg
      181 ccccaccaag gacgtcaccc gcaagcacca gatcatcatc tccccctccg gcctgatcac
      241 cgtgaccggg ggcaagtgga ccatctaccg caagatggcc gaggacgcca tcgacaaggc
      301 ggtgcaggtg ggctgcctcc cggagaaaga gtgcgtcacc aaaaccctgc cggtgcacgg
      361 ctatctgcgc aacatggatt tcgacgcccc gttgagctat tatggatcgg accgggtggt
      421 gctggaggag acggtgatgg cggaggagcc cgccctgggc gaacggatcc atcccgcttt
      481 tccctatatc agggcggagg tggtctgggc agtgcggcat gagatggcgc ggacggtgga
      541 ggattttctg gcgcgccggg tgcgggcgct ctttctcgat gcgcgcagca gcatcgcggc
      601 ggccccggcg gtggcggaga tcatggcggc ggagctgggg agggacgcgg cctgggcccg
      661 cggccaggtg gaggagtata cagagctggc gaagggatat atcgtgggat aaaacctgcc
      721 ggccagggtg gtaacgagat tatatttctc gtccccacct gaattcctgt taccggccaa
      781 ggtggacgtg ataatttttt ctttgtggct tggtggcttg gtgagagcct gtttttctcg
      841 tccccacctg aactcctgtt tctgcaaagg tggacgcgag aagtcaggca aaccgctcca
      901 gcagaaatgt ggagagttcc gccagctccg ggcccatcgc cagcgcctgt ttgggcaggg
      961 ccaggcagcg cgccagccgc tcgggcagct ccggctcgtg gccggtggcc ttttttaccg
     1021 tttcggcgaa tttgcccgga tgggccgtgg caagaaccac cacctgctcc cctccccttt
     1081 tgctcccctc gataaaatcc tctgcaaccg cacaccccac cgccgtatgg ggatcgacca
     1141 gcattccgaa gcggctggag agctgatgca tgctccagag ggtgcgctcg tcgtcgacca
     1201 caccgctgcc caccacagcc ttcatccccc gccagttgcc atcaaatagc tgctccatgc
     1261 gttcgaaatt gccgggatga ccgacatcca tggcattgga gagggtgcgc agcgaggggc
     1321 gcggggtgta gaccccgctc ttgagataat cgggaacgac atcgttgatg ttggtggcgg
     1381 cgagaaagcc ggccgcctcc attccccagt gccaggcgta gagtccggcg gtgaggttgc
     1441 cgaaattgcc gctcggcacg caaaagcgcg ggggcgctcc agagagcact ctgcgctgtg
     1501 cggctgcgtg gatgaagtaa aaactctggg gcaggaggcg gccgatgttg atcgagttgg
     1561 ccgaggtgag gcgcagggcg gcgcgcaggc ggcggtcgcg gaaggcggcc ttgaccaggc
     1621 gctggcagtc gtcgaagctg cccctgacct cgatggcgtg gacattgccg ccgagggtgg
     1681 tgagctgctg ctcctgcagg gcgctgaccc gtcccgaggg atagaggagg atgacgtcga
     1741 tgttggcgcg attgtgaaag gcgttggcca cggcgctgcc ggtgtcgccc gaagtggcga
     1801 ccaggatctg gcagcgctcc ccctcaagca gttcctccat gctggcggcg agaaagcggg
     1861 cgccgaaatc cttaaaggcg caggtggggc catggaagag ttcgaggagc agacggccgc
     1921 catcgatgcg caccaggcgg ggggcgaaag agaaggcgcg gccggcgatg cgccgggcgg
     1981 ccgcctcatc gatctcgggg gcaagcaggg ccgcgctgac gagggcggcc acatcgttaa
     2041 aagtgacgga ctcgtcaaat gcggcgatga gggcggagag atcggggtga tcgacgggat
     2101 ggtagagtcc tccgtcgggg gcgatacctg aaaagagggc ctggcggaag gtggcctgat
     2161 cgtgcggatg gaggcggctg gcgaatttca tgcgttttct cccgaggctg gctggtttgg
     2221 gctggtctcc tcatcgagca ccgccggtcc gtcgggattg acccgcgaga ggaagaggtc
     2281 ggcggcgagg cccgcctgtt caaaagcgct tttcatggcc tccccggccc gggcggccgc
     2341 ggcgaggtcg gcgcagaggg cgaagagaga ggggcccgaa cccgagatcg agcagcccag
     2401 ggccccggcg gcgaggg
//
LOCUS       DAOPWU010000461         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_46220_length_2414_cov_4.362866, whole genome shotgun sequence.
ACCESSION   DAOPWU010000461 DAOPWU010000000
VERSION     DAOPWU010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46220_length_2414_cov_4.362866"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKM23_15135"
     CDS             complement(<1..188)
                     /locus_tag="PKM23_15135"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNY92856.1"
                     /translation="MKAAIAIISGRPNSGKTACLLAVIARARRLGLTCGGLVSHGLWN
                     DGRKTGYMLEAVATGERRL"
     gene            complement(185..772)
                     /locus_tag="PKM23_15140"
     CDS             complement(185..772)
                     /locus_tag="PKM23_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92857.1"
                     /translation="MKRSLLYLLLGLGAALLLRESNQLYLFPAALLLLTLIDPRAWRI
                     FRRWKLWLFFFFVVAVPVLFVGTRDALWLTIPYNSRMLALNLVMLERSIIIMLAIRMF
                     TNRLSPEALSRGLARLRLQQFDEVFGLARTMLPELQETVGAELKGVEWRKALRRPSGL
                     IAQLGALVARILFATRGGAVRNTTDGSDDSAENQR"
     gene            complement(769..1386)
                     /locus_tag="PKM23_15145"
     CDS             complement(769..1386)
                     /locus_tag="PKM23_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92858.1"
                     /translation="MQETASVKGSAHEEQVIIALFGALWGLMEITLGALLKGTRLPFS
                     GAILACLAAVIALTGRSFVQRRGAILMMGGVAALIKIFSVGTVIAGPFFAILIEAAIA
                     ESLVLAFGLGRTGCLLAGAGMTTYTVFHPLLTQGLFFGGRIYEVYWATARQVGEWLGL
                     EVAHLALFIALYLGVHALTGALAGWFAWAVARTARLELEKLERSA"
     gene            1690..>2414
                     /locus_tag="PKM23_15150"
     CDS             1690..>2414
                     /locus_tag="PKM23_15150"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNY92859.1"
                     /translation="MRACLLAGIGEFALQELPDPQIRHAREVLVRIRMVGVCGSDIHY
                     FTSGRIGDAVIHYPFIIGHEAAGVVEAVGPGVSRLRPGQKIAIDPALSCGRCDQCLAG
                     RENTCRHLLFRGAPGQLPGCMCEYVVLDERQCFPVSDALSFELAALSEPLAVGVYAVE
                     RSRIDAGAHAAVLGAGPIGLSILHLLAARSHGPLFVTDKVEARLDYARQLGPLWAGNP
                     DRVEVEAEIGRLAPRQLDMVYECT"
BASE COUNT          460 a          765 c          780 g          409 t
ORIGIN      
        1 agccgcctct cgccggtcgc gaccgcttcg agcatatagc cggttttacg cccatcgttc
       61 cacagaccgt ggctgacgag accgccgcag gtcagaccga ggcggcgcgc ccgtgcgatg
      121 accgcgagca ggcaggcggt cttgccgctg ttgggccggc ctgatatgat ggctatggct
      181 gccttcatct ttggttttcc gctgagtcat cgctcccatc cgtggtattc cggacggcgc
      241 ctcccctggt ggcgaacaga atgcgggcga ccaaggcgcc gagctgggcg atcaggcccg
      301 agggacggcg gagggccttg cgccactcca cccctttcaa ctccgcgccc accgtctcct
      361 gcagctccgg cagcatggtc cgggccagac caaaaacctc atcgaactgc tgcagccgca
      421 ggcgggccag cccccgcgaa agcgcctccg gcgaaagccg gttggtgaac atcctgatcg
      481 ccagcatgat gatgatgctg cgctccagca tgaccaggtt cagggctagc atgcggctgt
      541 tgtaggggat ggtgagccag agcgcatccc tggttccgac gaacagcacc gggacggcga
      601 cgacgaagaa gaaaaagagc cacagcttcc agcgcctgaa aattcgccag gcgcggggat
      661 cgatcagggt gagcaggagc agggcggctg gaaaaagata gagctgattg gattctctca
      721 gcagcagggc ggcaccaagt ccgaggagga gataaaggag ggaccgcttc atgcggatcg
      781 ctcgagtttt tccagttcca gcctggctgt gcgtgctacg gcccaggcga accagcccgc
      841 cagagcgccg gtgagggcat gaacccccag atagagagcg atgaaaaggg cgagatgggc
      901 tacctccagc ccgagccatt cccccacctg acgggctgtc gcccagtaga cctcatagat
      961 ccggccgccg aaaaaaagcc cctgggtcag gaggggatga aagacggtat aggtggtcat
     1021 gccggcgccc gcgagcaggc agcctgtccg gccgaggccg aaggccagga ccagggactc
     1081 ggcaatggcc gcttcgatca ggatggcaaa aaagggcccg gcgatgacgg tgccgaccga
     1141 aaaaatcttg atcagggccg ccaccccgcc catcatcagg atggcgccgc ggcgctgcac
     1201 gaaggaacga ccggtcaggg cgatgacggc agcaaggcag gccaggatag ccccgctgaa
     1261 gggcaggcgg gttcccttga ggagggcgcc gagggtgatc tccatcagtc cccagagggc
     1321 gccgaagagg gcgatgatca cctgctcctc atgagccgat cccttcacgc ttgccgtttc
     1381 ttgcacctgc actcctttca ccatgctgaa ttagcgggac tgacaaggcg gccccacccc
     1441 gcctgggcaa ccaaccccga tcagcacaaa ttaacgctgt tgggggcaat aaagcaagcg
     1501 gtatttacac cgcactcgtc tccgaacccc gccctgcggc agaattatcc cgagtttccg
     1561 cttgaattat tggcggtaaa ttgctaacat attcttttcc attgaataca ccatgggcaa
     1621 attctccctg aagagcgcag gaggaggccc gctgccgttt cctcgacaaa atctacggaa
     1681 aggaatagaa tgcgcgcctg cctgctcgcc ggcatcgggg aattcgccct gcaggagctc
     1741 cccgatccgc agatcagaca tgcccgggag gtcctcgtcc gcatccgcat ggtcggggtc
     1801 tgcggttcag acatccacta tttcacctcc ggacggatcg gggatgctgt tatccactac
     1861 cctttcatca tcggccacga ggccgctggg gttgtcgagg ccgtcggccc gggggtaagc
     1921 cggctgcggc cgggtcaaaa gatcgccatc gatccggccc tctcctgcgg gcgctgtgac
     1981 cagtgcctgg cggggcggga gaacacctgc cgacatctgc tcttccgcgg cgcccccggc
     2041 cagctgccgg gctgcatgtg cgagtacgtc gttctcgacg agcgccagtg ttttccggtg
     2101 agcgatgccc tgagctttga gctggcggcg ctctccgagc ccctggcggt gggggtttat
     2161 gcggtcgagc gctcgcggat cgacgccggg gctcatgccg cagtcctcgg tgccgggccg
     2221 atcgggctgt cgattctcca ccttctcgcc gcccggagcc atggaccgct cttcgtcacc
     2281 gacaaggtcg aggcgcgcct ggactatgcg cggcagctcg ggccgctgtg ggcgggcaat
     2341 ccggatcggg tggaggtcga ggcggagatc ggccggcttg cgcccaggca gctcgatatg
     2401 gtctacgagt gcac
//
LOCUS       DAOPWU010000462         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_46286_length_2412_cov_3.557488, whole genome shotgun sequence.
ACCESSION   DAOPWU010000462 DAOPWU010000000
VERSION     DAOPWU010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46286_length_2412_cov_3.557488"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PKM23_15155"
     CDS             <1..943
                     /locus_tag="PKM23_15155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNY92860.1"
                     /translation="KKIVREFGLSDADLSKIRGRGENQRITKQDIMDYLSSKGVDTRM
                     EEVQPPAQTATVTPLPPLPAAQPLPQPPAMTSGAPAGASPATDQEIVPMDHVRKRIAE
                     HMIISKQTSAHVTSVAEVDVTPLVQYREGIKRTFEAREGFPLTYTAFFIEAAARALRE
                     FPLINASLDGDKIIIKKSIHIGIAVGLEDKLIVPVIRDAGRLNLAGIARAVHDLATRA
                     RNRQLKPDEVQGGTFSITNIGTFGNLWGTPIINQPQVAILGTGAIKKRPVVVNDAIAI
                     RSMMYLSLTYDHRLIDGLYGGRFLQMIVQLLENYKFD"
     gene            1017..1652
                     /gene="thyX"
                     /locus_tag="PKM23_15160"
     CDS             1017..1652
                     /gene="thyX"
                     /locus_tag="PKM23_15160"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNY92861.1"
                     /translation="MDFMGGDLAVVQAARVSFGKESTDPERDHKLLDFLLANRHETPF
                     EHSVFKFHIKCPIFVARQWFRHRIASYNEISGRYATMQEEFCLPDHLRTQKTRNYRYD
                     DLAEEDNIELRARMASFYQSAYELYQELLERGVAREHARLVLPLSLYTQFYWTVNARS
                     LMNFLSLRTEEHAQYEIRLYAEAINAIFREKMPWTSEAFGKYILAVQKHGE"
     gene            1754..>2412
                     /locus_tag="PKM23_15165"
     CDS             1754..>2412
                     /locus_tag="PKM23_15165"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNY92862.1"
                     /translation="MQSANETRRPHWLKVDLTGKGAFYSVRQLVDVQNLHTVCQSARC
                     PNIGECWAKKTATFMILGDSCSRNCTFCAISHGALEAVDPDEPRRVAEAVKSLDLRYA
                     VVTSVTRDDLPDGGAGAFARTIAAIRTANPGCRIEVLIPDFAGSKEALMTLLAQNPDV
                     LNHNLETVEELYPRVRPQANYHRSLDLLDSARRHGATTKSGLMLGVGESRDQLIRTLR
                     DL"
BASE COUNT          517 a          798 c          623 g          474 t
ORIGIN      
        1 taaaaaaatc gtgcgggaat tcggcctttc cgatgccgac ctgagcaaga tcaggggccg
       61 gggcgaaaac cagcgcatca ccaaacagga tatcatggat tacctctcca gcaaaggagt
      121 cgacacccgc atggaagagg tccagccccc tgcacagacc gccacagtta cgcccctgcc
      181 gcctcttccc gccgctcagc cgctccccca gccaccggcg atgacgagcg gagcacctgc
      241 tggtgcttcg cctgcaaccg atcaggagat cgtgccgatg gatcatgtgc gcaagcgcat
      301 cgccgaacac atgatcatca gcaaacagac ttcggcccat gtcacctcgg ttgctgaagt
      361 cgatgtcacc cccctggttc agtaccgtga gggtatcaag cgcactttcg aggctcgcga
      421 gggcttcccg ctaacctaca ccgccttttt tatcgaggcc gccgcgcgcg cgctcaggga
      481 gtttcccctc atcaatgctt cgctcgacgg ggataaaatc atcatcaaaa agtcgattca
      541 tattggtatc gcggtcggcc tcgaggataa gctcattgtg cccgtcatcc gcgatgccgg
      601 ccgcctcaat ctcgccggaa tagcccgcgc cgtccatgat ctggccaccc gggcgcgcaa
      661 ccgccagctc aagccggacg aggtgcaggg cggaaccttc tccatcacca acatcggcac
      721 cttcggcaac ctctggggga ccccgatcat caatcagccc caggtggcca ttctgggtac
      781 gggcgcgatc aaaaaacggc ccgtcgtcgt caacgacgcc atcgccatcc gctcgatgat
      841 gtatctcagc ctgacctatg accaccgcct tatcgatgga ctctacggtg gcaggttttt
      901 gcaaatgatt gttcaactcc tcgaaaatta taaatttgac tgatcactgg aaaggcagga
      961 ttgagttgca ggtactcaac gatattcccg tgctcgatca cggtttcgtc aggctcatgg
     1021 actttatggg cggtgatctg gcggtggtcc aggccgcccg ggtctccttt ggcaaggaat
     1081 ccactgatcc ggaaagggac cacaagctgc tcgactttct cctcgccaac cgccacgaga
     1141 ctccctttga acactcggtc tttaaatttc atatcaaatg tcccattttc gtcgcccgcc
     1201 agtggttccg ccaccgcatc gccagctaca acgagatctc cggccgctat gccaccatgc
     1261 aggaggagtt ctgcctcccc gaccacctgc gcacacaaaa gacgcgcaat tatcgatatg
     1321 atgacctcgc cgaagaggac aacattgaac tgcgtgccag gatggcctcc ttttatcaaa
     1381 gcgcctatga actctaccag gagctgctcg agcgtggcgt cgccagggag cacgcccgcc
     1441 tcgtcctgcc cctctccctc tacacccagt tctactggac cgtcaatgcc cgctcgctga
     1501 tgaatttcct ctcgctgcgc accgaagagc atgcccagta cgagatccgc ctctacgccg
     1561 aggcgatcaa cgccattttc cgggaaaaga tgccatggac cagcgaagcc ttcggcaaat
     1621 acatcctcgc cgtccagaag cacggagaat gacaccgcag caccaccctc gttgaaaggc
     1681 cggaggcggt tgtggccgtc tccggctccg atattagtgt tgtccctcga aaaatatttt
     1741 tgtatattac acgatgcaat cagcgaacga aaccaggcgt ccccattggc tcaaggtgga
     1801 tctcaccggc aaaggcgcct tttacagcgt caggcagctg gtcgacgtcc agaatctgca
     1861 taccgtctgc cagagcgccc gctgccccaa catcggtgaa tgctgggcga aaaaaaccgc
     1921 cacctttatg atcctcggtg acagctgttc gcgcaactgc accttctgcg ccatctcgca
     1981 tggcgctctc gaggccgttg atcccgatga accgcggcgc gtcgccgaag cggttaaatc
     2041 cctcgacctc cgttatgcgg tggtcacttc ggtcacccgc gatgatctgc ccgatggcgg
     2101 ggccggcgcc tttgcacgga ccatcgcggc catccgcaca gccaatcccg gctgccggat
     2161 cgaggtgctg atccccgatt tcgccggctc aaaggaggcc ttgatgaccc tgctggcgca
     2221 gaaccccgat gtgctcaacc acaatctcga aaccgtcgag gagctctatc ccagggtcag
     2281 accacaggcc aattaccacc gctccctcga cctcctcgac tctgcacgcc ggcacggcgc
     2341 cacgactaaa tcggggctga tgctgggcgt gggagagagc cgcgaccagc tgattagaac
     2401 cctcagggat ct
//
LOCUS       DAOPWU010000463         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_46320_length_2411_cov_3.957555, whole genome shotgun sequence.
ACCESSION   DAOPWU010000463 DAOPWU010000000
VERSION     DAOPWU010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46320_length_2411_cov_3.957555"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PKM23_15170"
     CDS             complement(<1..449)
                     /locus_tag="PKM23_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92863.1"
                     /translation="MRKRLFPFTVICILLVASSTSPAAEPGPGGLVQLEEKEGRLCGT
                     PIIAQQQQLLIENTRRLSPELPAALAKEAGVSHVVGDTLSFFTVDFSTSAYSTIKAVC
                     REKTAQTYIFVGAEDLASNRVSAADIADFKTAFESRTPAGSLDPNQG"
     gene            629..1558
                     /locus_tag="PKM23_15175"
     CDS             629..1558
                     /locus_tag="PKM23_15175"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNY92864.1"
                     /translation="MIETVFTRAQDGFRWLDSVQPSSEELGSIAETYTLHPTSVQDCL
                     QPEHLPKFERFDNAYFLIVRGYDENCPGDADTVQELTRKTAIFWNSHFFITIHRQDQL
                     FMEGMRRLWSAPGVQIPAEPSIPLLHDLIKRVLISYEAPLEAADSRLDEYEEKVFISQ
                     SEARILQELYLLKRKATVFKKMIFLTKDVLKSLNIESRQHRPLVQDLIETADRFFFLA
                     DQLLENTNTLLNMHISLSSQRTNEVMRVLTLFSVFFLPLTFIVGIYGMNFDFMPELRH
                     PFGYPAILVVMMLIVLGVFLWFRRKGWLGKRGE"
     gene            complement(1661..>2411)
                     /locus_tag="PKM23_15180"
     CDS             complement(1661..>2411)
                     /locus_tag="PKM23_15180"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNY92865.1"
                     /translation="VNVETGQLFGTREASSKQELKHCEDERSTEMAPADQTVDLCLQT
                     IAKELIDYFMPRFQQQKFEFAKIEGKEFKRPADAARESIDGYDLHSAFINYSAILEQD
                     PYNHAAIFNLGVLHEVVGNYALAVEKYRTAASLVSREEKYRKALQRVEKQRLYWDELK
                     GLGLELKEYDFSFTEKQASSTLSRRIRINGQGSERIPLYEKPDDSSKVLVRVPGGVEL
                     ETASTAGEWYQVKLLDGRLAWIHEQEVEEVD"
BASE COUNT          487 a          723 c          672 g          529 t
ORIGIN      
        1 atgccctgat tggggtcgag tgagccggca ggggtgcggg attcgaaggc ggtcttaaag
       61 tccgcaatat cggctgcgct cacccggttc gacgccagat cctcagcgcc cacaaagata
      121 taggtctgtg cggtcttttc ccggcacacc gccttgatgg tggaataggc ggaagtgcta
      181 aagtcgaccg taaaaaagct gagggtatcc ccgaccacat gggatacccc ggcttccttg
      241 gccagggcag caggcagttc cggtgacagc cgccgcgtgt tctcgatgag aagctgctgc
      301 tgttgcgcga tgatcggggt gccgcagagc cgcccctctt tctcctccag ctggacgagg
      361 ccgccgggac cgggttcggc ggccgggctg gtactggagg ccaccaggag gatgcatatg
      421 acggtgaaag ggaagagtcg ttttcgcata tcaatcctgt aagctgcaat ggagatgaaa
      481 gccgctgtct tcttctgatg gggtaaggta cgataatttg ccacttgtcg caagattttt
      541 ttcggcagct ggatgacaaa tacctttttt tcaatcaatg gattggttaa attaaaagaa
      601 tagaagagaa gcaacatgga tcacagccat gattgaaacc gtttttaccc gcgcacagga
      661 tggattccgc tggcttgatt cggtccagcc cagcagcgaa gagctgggct cgatcgcgga
      721 gacctacacc ctccatccca catcggtgca ggactgcctg cagccggagc atctgcccaa
      781 attcgaacgc tttgacaatg cctactttct aatcgtccgc ggctatgacg agaactgccc
      841 cggtgacgcc gacaccgtcc aggagctgac acgcaagacc gccatcttct ggaactccca
      901 ttttttcatc accatccatc gccaggacca actcttcatg gaaggtatgc ggcgcttatg
      961 gagcgcgccc ggggtgcaga ttccggccga accctcaatc ccccttttgc atgatctgat
     1021 caagcgggtc ctgatcagct acgaggcccc cctcgaagcg gccgacagcc ggctcgatga
     1081 atatgaggag aaggtcttca tcagtcagtc cgaggcccgc atcctgcagg agctctacct
     1141 gctcaagcgc aaggcgacgg tctttaagaa gatgatcttc ctgaccaagg atgtcctcaa
     1201 gagcctgaac atcgaatcgc gccagcaccg ccccctggtg caggacctca tcgagaccgc
     1261 cgaccggttt ttcttcctcg cggaccagct gctggaaaat accaacaccc tgctcaacat
     1321 gcacatctcg ctctcttcgc agcggaccaa cgaggtgatg cgcgtcctca ccctcttttc
     1381 ggtctttttc ctgcccctga ccttcatcgt cggcatctac ggcatgaact ttgactttat
     1441 gcctgaactc cgccatccct tcggctatcc ggccattctg gtcgtcatga tgctgattgt
     1501 gctgggtgtc tttctctggt tccgccgcaa gggctggctg ggcaagcggg gagaatagga
     1561 gagatagatc tgaccggggt tgagccgcgg ccccggtcag agcgacgcga aaatgaaccg
     1621 gcagtatggg ctctcaggca ggcggtgccg gcgagacgcc tcaatccacc tcttcgacct
     1681 cctgttcgtg aatccaggcg agacggccgt ccagcaactt gacctgatac cactctccgg
     1741 ctgtggaggc ggtctccagc tcgacaccac caggcacgcg caccagcacc ttgctgctgt
     1801 catccggctt ttcatagaga ggaatgcgct ccgagccctg gccgttgatg cggatccggc
     1861 gcgagagagt cgaggaggct tgtttctcag tgaacgagaa atcatattct ttcagctcca
     1921 gccccagccc cttcagttca tcccagtaga gccgctgctt ctccacccgc tgcagggcct
     1981 tgcggtactt ttcctccctg ctaaccaggc tggcggcagt ccggtacttt tccaccgcca
     2041 gagcataatt gccgaccacc tcgtgaagta cacccaggtt gaaaatcgcc gcatggttgt
     2101 agggatcctg ttcgaggatg gcactgtagt tgataaaggc cgagtgcaga tcataaccgt
     2161 cgatgctctc ccgtgcggca tcagccgggc gcttgaactc cttgccctcg atcttggcaa
     2221 attcaaactt ttgctgctgg aagcggggca tgaagtaatc gatcagctcc ttggcgatcg
     2281 tctgcagaca gaggtcgacg gtctggtcgg ccggcgccat ctctgtactg cgctcgtcct
     2341 cgcaatgctt gagctcctgc ttgctgctgg cctcccgggt gccgaagagc tggccggtct
     2401 cgacgttgac g
//
LOCUS       DAOPWU010000464         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_46385_length_2409_cov_4.061597, whole genome shotgun sequence.
ACCESSION   DAOPWU010000464 DAOPWU010000000
VERSION     DAOPWU010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46385_length_2409_cov_4.061597"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PKM23_15185"
     CDS             <1..733
                     /locus_tag="PKM23_15185"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNY92866.1"
                     /translation="RKKNDIVSIPIGCTEQHGDHTVTALDTFMVTQILEGVRRYTAKK
                     GAACNLVFSPLNYGGHPHHHIGMPGTVHLEDEVVKRNLIDVMVGLWNDGFRKQILVNN
                     HGHSWLMEAAIQELQKRWNLPGIFRTLDWHRSVREFFRTKARGGEWDDDFVHADEAET
                     SVMLLLEPRFVEMNYAEETKVEAFCRTAISTRRSIPSAGRAAGARARATPRSSSPRCP
                     RGWLDARPSAAHRRPNARSRPSSPI"
     gene            661..894
                     /locus_tag="PKM23_15190"
     CDS             661..894
                     /locus_tag="PKM23_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92867.1"
                     /translation="MVGRPTIGSAQKAKRTIAAILSYLTLINDEILAAFPPGQLPPVE
                     KTTMRTAAEMEPYLREPFSPGWRPIYSIQKMGL"
     gene            894..1700
                     /gene="iolO"
                     /locus_tag="PKM23_15195"
     CDS             894..1700
                     /gene="iolO"
                     /locus_tag="PKM23_15195"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF041099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-keto-L-gluconate epimerase"
                     /protein_id="HNY92868.1"
                     /translation="MRCRLSIVLSLQPASFAAMVYRGGLEEDLGRIKALGYDGVELAI
                     RDPGLLDRGRVLSALEAAGLPVPAIGTGQAFGEEGLSFTDPDRNVRARAVARMHAHVP
                     LAEKLGALLIVGLIRGKSAGAQDKSRAEELILEAMRDIALGHPAQKFAVEPINRYETE
                     FINTVDAGLRFLDRLKLDNVGLLLDTFHMNIEEPSPTRSIAMAGERIFHFHIADSNRW
                     HPDAGHIDFGAVIEALDRAGYRGWLSAEILPLPDADSAARNTIVHMRRFT"
     gene            1697..>2409
                     /locus_tag="PKM23_15200"
     CDS             1697..>2409
                     /locus_tag="PKM23_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY92869.1"
                     /translation="MISGRPVLEMHHIGQVFPGVVALDDVHFDLMQGEVHVLLGENGA
                     GKSTLVKILSGANPRSSGQILLDGVEVRIETPRRAQELGIAIIYQELNLIPALTAAEN
                     IFLGREPRRRSGFIDQKRVVTDAQSLLDELHAGIDASRRVCELSVAQQQMVEVARALS
                     LQARILIMDEPTSALTGQEIGELFALIRRLTQRGVSVIYISHRMEEIFEIGEQVTVLR
                     DGRHIETRPIAGTSREELVR"
BASE COUNT          440 a          821 c          734 g          414 t
ORIGIN      
        1 ccgcaaaaag aacgacatcg tttccatccc tatcggctgc accgagcagc acggcgacca
       61 caccgtcacc gccctcgaca cctttatggt gacccagatc ctcgaggggg tccgccgcta
      121 caccgccaaa aagggagcgg cctgcaatct ggtcttttcg cccctcaatt acggcggcca
      181 tccccaccac cacatcggca tgcccggcac cgtccatctc gaggatgagg tggtcaagcg
      241 caacctcatc gatgtcatgg tcggcctctg gaacgacggc tttcgcaaac agatcctggt
      301 caataaccac ggccactcct ggctgatgga ggcggcgatc caggagctgc agaaacgctg
      361 gaacctgccg ggtattttcc gcaccctcga ctggcaccgt tcggtgcgcg agttcttccg
      421 caccaaggcg cgcggcgggg aatgggatga cgattttgtc catgccgacg aggctgagac
      481 ctccgtgatg ctgctgctgg agccgcgctt cgtcgagatg aactatgccg aggagaccaa
      541 agtggaggct ttttgccgga cggccatttc gacaaggcgg tcgatccctt cggccggccg
      601 agccgctgga gcgagggcca gggccactcc ccgatcgagc tcgcctcggt gcccgagggg
      661 gtggttggac gcccgaccat cggcagcgca cagaaggcca aacgcacgat cgcggccatc
      721 ctctcctatc tgaccctgat caacgacgag atcctcgccg cctttcctcc ggggcagctg
      781 ccgccggtcg aaaagaccac catgcgcacg gccgccgaga tggaacccta tctcagggag
      841 cccttttcgc cgggctggcg gccgatctac agcatccaga aaatggggct ctgatgcgct
      901 gcagactgag catcgtcctc tcgctccagc ccgcctcgtt tgcggcgatg gtctaccgcg
      961 gcggactgga ggaggacctg ggccggatca aggccctcgg ctacgacggc gttgaactgg
     1021 ccatccgcga tcccggtctg ctcgaccggg gccgggtgct gtcggccctc gaagccgccg
     1081 ggctgccggt gccggccatc ggcaccggac aggccttcgg ggaggagggg ctctctttca
     1141 ccgatccgga ccggaacgtg cgcgcccgcg cagtggcgcg catgcacgcc catgtgcccc
     1201 tggcggagaa gctgggggcc ctcctcatcg tcggcctcat ccgcggcaaa tcggccggcg
     1261 cccaggacaa atcccgggcg gaggaactca tcctcgaggc gatgcgggac atcgccttgg
     1321 gccatcccgc gcaaaaattc gccgtcgagc ccatcaaccg ctacgagacc gaattcatca
     1381 acaccgttga tgccggactg cgctttctcg accgcctcaa gctggacaat gtcgggctgc
     1441 tgctcgacac ttttcacatg aatatcgaag agccctcccc gacccgcagc atcgccatgg
     1501 cgggggagcg gatttttcat tttcacatcg ccgactccaa ccgctggcac ccggacgccg
     1561 gccacattga cttcggcgcg gtcatcgagg ccctcgaccg cgccggctac cgcggctggc
     1621 tctccgccga gatcctgccc ctgccggacg ccgacagcgc cgcccgcaac accattgtgc
     1681 atatgcggag gttcacgtga tcagcggccg gcccgtgttg gagatgcacc acatcggcca
     1741 ggtttttccc ggtgttgtgg ccctcgatga cgtccatttc gatctcatgc agggcgaggt
     1801 ccatgtgctg cttggggaaa acggcgccgg aaaatcgacc ctggtcaaga tcctcagtgg
     1861 cgccaatccg cgcagcagcg ggcagatcct gctcgatggt gtcgaggtga ggatcgaaac
     1921 cccgcgccgg gcccaggaac tgggcatcgc catcatctac caggagctca atctcattcc
     1981 cgccctcacc gcagcggaga acatttttct cggccgcgag ccgcggcgcc gctccggatt
     2041 catcgatcag aaaagagtgg tcactgatgc ccagtccctg cttgacgaac tccatgccgg
     2101 gatcgacgcc agcaggcgcg tgtgcgagct cagcgtagcc cagcagcaga tggtggaggt
     2161 ggcgcgggcg ctttcgctcc aggcccgcat tctcatcatg gatgagccca cctcggcctt
     2221 gaccgggcag gagatcggcg agctcttcgc cctgatccgc cgcctgacac aacggggggt
     2281 atcggtcatt tacatctcgc accgcatgga ggagatcttt gagatcgggg agcaggtcac
     2341 cgtgctgcgc gacggccgtc acatcgaaac cagaccaatc gccggcacct cccgggagga
     2401 actggtccg
//
LOCUS       DAOPWU010000465         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_46534_length_2404_cov_4.446999, whole genome shotgun sequence.
ACCESSION   DAOPWU010000465 DAOPWU010000000
VERSION     DAOPWU010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46534_length_2404_cov_4.446999"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2091
                     /gene="gcvP"
                     /locus_tag="PKM23_15205"
     CDS             <1..2091
                     /gene="gcvP"
                     /locus_tag="PKM23_15205"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNY92870.1"
                     /translation="IVLGSSQRFGVPMGFGGPHAAYFATRSEFMRQMPGRIIGVSIDR
                     HGKRSYRMTLQTREQHIRREKATSNICTAQALLAIMAGMYGVWHGPEGIKAIAGRIHL
                     LTRALAAALAELDYDQSNPVYFDTLLVHAGGARERIRELALKNGINFRYAGEEQIGIA
                     LDETVQPADVEEIVAIFAAARGQQAPLINWEKEAALPADTITGSLKRVTAYLTHPVFS
                     AYRSEHKMVRFLKRLENKDLSLTTSMIPLGSCTMKLNGTAEMLPLTWPGFSKLHPFVP
                     ADQARGYLELVHELERDLCRITGFAAASFEPNSGAQGEYTGLMVIRAWHHDHGQAHRD
                     VTLIPASAHGTNPASAVMAGTRVVVVACDDKGNIDLNDLQAKAEQYRENLSALMVTYP
                     STHGVFEEKIVEICKIIHANGGQVYMDGANMNAQVGLTNPAAIGADVCHLNLHKTFAI
                     PHGGGGPGMGPICVAEHLAPYLPGHPLVEVGGGKAIPAVSATPYGSASILLISYAYIK
                     LMGSAGLTEATKYAILNANYIAARLGKEYKVLYTGDRGRVAHEMIFDLAEFKAQGIEA
                     EDIAKRLMDYNFHAPTVAFPVHNSIMVEPTESEDKGELDRFCDALSAIRREIQAVLDG
                     VADPADNVLKKAPHTAAEVCADGWSHPYSREEAAYPVAWLREHKFWPAVGRIDNTYGD
                     RNIFCACPPMEEYE"
     gene            2203..>2404
                     /locus_tag="PKM23_15210"
     CDS             2203..>2404
                     /locus_tag="PKM23_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011171451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNY92871.1"
                     /translation="MKEFRFHGRGGQGTVLASIALAKAFFQAGYWVQTFPVFGSERRG
                     APVEAYLRLETTKIYLRSNIYEP"
BASE COUNT          462 a          867 c          668 g          407 t
ORIGIN      
        1 atcgtcctcg gctcgagcca gcgcttcggc gtgcccatgg gcttcggcgg accgcacgcc
       61 gcttatttcg ccacccgcag cgagttcatg cgccagatgc cgggccgcat catcggagtc
      121 tcgatcgacc gccacggcaa gcgctcctac cgcatgaccc tgcaaacccg cgaacagcac
      181 atccgccgcg aaaaagcgac ctccaacatc tgcaccgccc aggccctgct ggcgatcatg
      241 gccggcatgt acggggtctg gcacggcccc gaggggatca aggccatcgc cggccgcatc
      301 cacctcctca cccgcgccct cgccgccgcg ctcgccgaac tggactatga tcagagcaat
      361 cccgtctatt tcgacaccct cctggtccac gccggcggcg ccagggaacg gatccgcgaa
      421 ctggccctga agaacgggat caatttccgc tatgccggcg aagagcagat cggcatcgcc
      481 ctcgacgaga ccgtccagcc tgccgacgtt gaggagatcg tcgccatctt cgccgccgcc
      541 cgcggccagc aggccccgct catcaactgg gagaaggagg ccgcccttcc cgccgatact
      601 atcaccggca gcctcaagcg cgtcaccgcc tatctcaccc atcccgtctt cagcgcctac
      661 cgctccgaac acaagatggt ccgtttcctc aagcgcctcg agaacaagga cctctccctg
      721 actacctcaa tgattcccct cggctcctgt acgatgaagc tcaatggcac ggccgagatg
      781 ctgccgctga cctggcccgg cttctcgaag ctgcatccct ttgtgcccgc cgaccaggcc
      841 cggggctatc tggagcttgt ccacgaactg gagcgcgacc tttgcagaat caccggcttc
      901 gccgccgcct ccttcgagcc caactccggg gcccagggcg agtacaccgg cctgatggtg
      961 atccgcgcct ggcatcatga ccacggccag gcccatcgcg acgtcaccct cattcccgcc
     1021 tccgcgcacg gcaccaatcc cgccagcgca gtcatggccg gcacccgggt cgtggtcgtc
     1081 gcctgcgacg acaagggcaa catcgacctc aacgacctcc aggccaaggc cgagcagtac
     1141 agggagaacc tctcggccct gatggtcacc tatccctcca ctcatggggt ttttgaggaa
     1201 aaaatcgtcg aaatctgcaa gatcatccat gccaacggcg gccaggtcta catggacggg
     1261 gccaatatga acgcccaggt ggggctgacc aatccggccg ccatcggcgc ggatgtctgc
     1321 cacctcaacc tgcacaagac cttcgccatt ccccatggcg gtggcggccc gggcatgggt
     1381 ccaatctgtg tggccgaaca tcttgccccc tatctgccgg gccatcccct ggtcgaggtc
     1441 ggcggcggca aggctatccc ggcggtcagc gccaccccct atggcagcgc cagcatcctg
     1501 cttatctcct atgcctatat caagctgatg ggcagcgccg gtctgaccga ggcgaccaaa
     1561 tacgccatcc tcaatgccaa ctatatcgcc gcccggcttg gcaaggagta caaggtgctt
     1621 tataccggcg atcgcggccg cgtcgctcat gagatgatct tcgacctggc cgagttcaag
     1681 gcccagggca tcgaagccga ggacatcgcc aagaggctga tggattataa ctttcacgcc
     1741 ccgacggtcg cttttccggt ccataactcg atcatggtcg aacccaccga gagcgaggac
     1801 aagggcgaac tcgatcgctt ctgtgacgcc ctcagcgcca tccgccgcga gattcaggcg
     1861 gttctggacg gcgtggcgga ccccgccgac aatgtgctca agaaagcccc gcacaccgct
     1921 gccgaggtct gcgccgacgg ctggagccat ccctactcgc gcgaagaggc ggcctacccc
     1981 gtcgcctggc tgcgggagca caagttctgg ccggcggtgg gacgcatcga caacacctat
     2041 ggcgaccgca atatcttctg cgcctgcccg cccatggagg agtacgagta accacccctc
     2101 ttctctgtcc gcccggctgg agaccatacc gccgggcgga cagcagacaa tcacggccac
     2161 gtaataaaac acctcttatc gtatagaaag agtccagatt ctatgaagga gttccgcttt
     2221 cacggccggg gaggacaggg caccgtgctc gcctccatcg cgctcgccaa ggccttcttc
     2281 caggcgggtt actgggtgca gaccttcccg gtctttggtt cggagcgccg cggcgccccg
     2341 gtcgaggcct acctccgtct ggaaacaacc aaaatctatc tgcgcagcaa catctatgag
     2401 cccg
//
LOCUS       DAOPWU010000466         2400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_46642_length_2400_cov_3.969723, whole genome shotgun sequence.
ACCESSION   DAOPWU010000466 DAOPWU010000000
VERSION     DAOPWU010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46642_length_2400_cov_3.969723"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKM23_15215"
     CDS             <1..244
                     /locus_tag="PKM23_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92872.1"
                     /translation="TRIRENSRTAAYRMIQELLGEVDFYSGELNGEQQATYAALVAFQ
                     QDYNTRVTENEQITSLGSFGYQTLEALRSRYRQLSR"
     gene            complement(327..2000)
                     /locus_tag="PKM23_15220"
     CDS             complement(327..2000)
                     /locus_tag="PKM23_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92873.1"
                     /translation="MKSANAIPFRLRIGVTGHRELADPEAIARKVKEFVEKELPELFD
                     NPAAVRAHLENPDRRTEIVYTVITPLAEGADRLVAHSILNIAPASRLRAVLPFPREEY
                     LKDFGSEASRREFESLYQLDADPLPPRNSLSLDNMDEKKRAQERSKAYLAAGVLVVEQ
                     CDLLIAVWDGEKARGKGGTAEIIAYAEERGRPLVKIFTSGRGNEISRGAGLKWQSLNR
                     IEEFNAHPITAEEISLYTENVYNQLFANPPGAMLDPAMLGEVRSRLLPGYARASIIAK
                     HNRKLYHRAGQIVYLLSPVAVLAVAIGVLFRRLAPYALALEALILILILLLIFSANRM
                     KVLKKWIENRALAEHIRAGIFTTACGFMPRSLANQAAQATILQSDDWIMRLLNEMMSG
                     FRPPSRCSDETCSAAISFVRTMWIDEQIAFHANTFTRSDRISRFLERAGMIIFLAAIA
                     AAIIHLLTLPSPDEIGVHLTEKLIIILAIVLPAAGASIGGFRTHGEYSRLAKRSQSMK
                     TVLTELRSRFVYFAAPETLAEYLREIESVMLEENKDWIVLMRHIELSPV"
     gene            complement(1997..>2400)
                     /locus_tag="PKM23_15225"
     CDS             complement(1997..>2400)
                     /locus_tag="PKM23_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92874.1"
                     /translation="SGYDLERMPPGSTREGRPAPCRIHPPLFMDMHHHAAWAQVDRLL
                     AGNRRPVLAFLCRDNDIANLDLAISIKLKGPKSLKKATFICRMYAETVSELNDMLDHR
                     LTPNQTRDVLLFPLQAELKRAFATEIFGGMP"
BASE COUNT          452 a          675 c          791 g          482 t
ORIGIN      
        1 gacacgcatc cgggagaaca gccggactgc cgcctaccgc atgatccagg aactgctcgg
       61 cgaagtcgat ttttacagcg gggagttgaa tggggaacag caggcgacct atgccgccct
      121 ggttgccttt cagcaggact acaacacccg ggttacggag aacgaacaga tcacctcgct
      181 tggttccttc ggataccaga ccctcgaggc tctgcgcagc cgctaccggc agctctcccg
      241 ttagggctgt cttcaagtca agcagcggct gtgccctggc ggcacagcca tccctcctaa
      301 tttcatctcc atcgccgtcg gcaccctcac accggtgaga gttcgatgtg cctcatcaaa
      361 acgatccagt ccttgttttc ctccagcatc accgattcga tctcccgcaa gtattccgcc
      421 agggtctcag gggcagcgaa atagacgaag cggctgcgca gctcggtaag cactgtcttc
      481 atgctctggc tgcgcttggc caggcgcgaa tactcgccgt gggtgcggaa gccgccgatt
      541 gacgcgcctg cggccggcag cacgatggcg agaattataa tcaatttttc cgtgagatgg
      601 accccgattt catccgggga gggcagggtg agcaggtgga tgatcgcggc ggcgatggca
      661 gccaggaaga tgatcatacc cgcgcgttcg aggaagcggc tgatccggtc cgaacgggtg
      721 aatgtgttcg cgtgaaaggc aatctgctcg tcgatccaca ttgtgcgcac gaaagagatg
      781 gcggccgagc aggtttcatc ggagcagcgg gagggcgggc gaaagccgct catcatttcg
      841 ttgaggagcc gcatgatcca gtcatccgat tgcagaatgg tggcttgcgc ggcctgattg
      901 gcgagtgagc gcggcataaa gccgcaggcg gtggtgaaga taccggcgcg gatgtgctcg
      961 gccagggccc ggttttctat ccattttttc aacaccttca tccggttggc ggaaaagatc
     1021 aacagcaaga tgaggatgag gatcagagcc tcgagcgcaa gcgcgtatgg ggccagcctc
     1081 cggaagagga cgccgatggc gacggccagc accgcgacgg gtgagaggag atagacgatc
     1141 tggccggcgc gatgatacag cttgcggttg tgtttggcga tgatcgaggc gcgcgcatag
     1201 cccggcagca gtcggctcct gacctccccg agcatggccg gatcaagcat ggcgccaggg
     1261 gggttggcga agagctggtt gtagacattc tccgtgtaga ggctgatctc ctctgcagtg
     1321 atgggatggg cgttgaactc ttcgatgcgg ttgagcgact gccacttgag cccggcaccc
     1381 ctgctgattt cattcccccg gccgctggta aagattttga ccaggggccg gccgcgctcc
     1441 tcagcgtagg cgatgatctc ggcggtcccg cccttgccgc gggctttctc gccatcccaa
     1501 acggcgataa ggaggtcgca ctgctcgacc accagcaccc ctgcagccag ataggccttg
     1561 ctccgctcct gcgcccgttt tttttcatcc atattgtcga gggagaggct gtttcgaggc
     1621 ggcaggggat cggcatccag ctgataaagg gattcgaact cgcggcgcga ggcttcactg
     1681 ccaaagtcct tgagatattc ctccctcggg aagggaagaa cggcgcgcag ccggctggcg
     1741 ggggcgatgt tcaggatgga gtgcgcgacc agccggtcgg cgccctcggc caggggggtg
     1801 ataacagtgt agacgatctc cgtccggcgg tcgggatttt caaggtgggc tcgcaccgcg
     1861 gcggggttat caaagagttc gggcagctcc ttttcgacga actccttcac cttgcgtgcg
     1921 atggcctccg ggtcagcgag ttcacggtgg ccggtcacgc cgatgcggag gcggaagggt
     1981 atggcgttgg cgcttttcat ggcatccccc cgaaaatttc tgtggcaaag gctcttttca
     2041 gctcagcctg cagcgggaag agcaggacat cccgcgtctg gttgggtgtc aggcgatggt
     2101 cgagcatgtc gttcagttca ctgaccgttt ctgcgtacat ccggcagata aatgtggcct
     2161 ttttaaggct tttgggaccc ttgagcttga tcgagatggc gagatcgaga ttggcgatgt
     2221 cattgtcccg gcagagaaag gcgagcacgg gacgtctgtt ccctgccagc agtcgatcca
     2281 cctgggccca ggctgcgtgg tggtgcatgt ccatgaagag gggaggatgg atccggcaag
     2341 gtgccggccg cccttcgcgg gtgctgccgg gcggcatccg ttcgaggtcg tagccggagg
//
LOCUS       DAOPWU010000467         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_46666_length_2399_cov_5.765358, whole genome shotgun sequence.
ACCESSION   DAOPWU010000467 DAOPWU010000000
VERSION     DAOPWU010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46666_length_2399_cov_5.765358"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..935
                     /locus_tag="PKM23_15230"
     CDS             345..935
                     /locus_tag="PKM23_15230"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNY92875.1"
                     /translation="MPRLEKWVGWSFAFLSPNTWTTLSLFVAFAAYIAVLYDRPFLGA
                     IIFAVSALMDLVDGKVARYKGTATRLGAFWDGTIDRFVDALMILCLFSLEFPPAILPV
                     GILLFLLLFFTLLPPFIVAYANHRGAVPDPTEKVIWRFAFRIEYIVLFIAAILINGWS
                     PHWSLYLVYAALVLMVATVIQSIILVFVKSGNYPQQ"
     gene            1095..>2399
                     /locus_tag="PKM23_15235"
     CDS             1095..>2399
                     /locus_tag="PKM23_15235"
                     /inference="COORDINATES: protein motif:HMM:NF040455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNY92876.1"
                     /translation="MSRTASVLLLVICAWMAPLTAQNGALQLHLTTLIQERLKQLPEE
                     RRLATAGGSENLSPDLFTLYGRRGFRPIWASESGIRAEADSLLALIRKADRHGLDPAS
                     YHERALNLLLPQLNSRLHAAPVDPAVLADLDLLLSDAYLVLGLHLAAGRFNPHDLDAE
                     WYLHKNEADMVTRLNGAAEAGELLEDLRRLPPQEEEYHHLLEAYGYYRQLAVEGGWPF
                     IADGRKLEKGMWDERVMQVRARLRATADLALEPAAVEGTYDEALERAVRRFQMRHGLE
                     PDGKVGKSTLAEMNVPVGKRIRQLAVNLERWRWLPRDHGRRYIRVNIADYTLQLFEAD
                     TVAMAMRVVVGKTYRRTPIFSDLMTYLVINPYWTVPNTILLNDIVPKALKNPDYIHKE
                     RIKIYAGWSENAPEIDPASVDWNRLSKNYQPYRLRQDPGPLNS"
BASE COUNT          423 a          809 c          668 g          499 t
ORIGIN      
        1 tcccacctga cattgggcgc tgcgtacccc ccgaaatctg gttcccacct gacatcgggc
       61 actgcgtacc ccaatccctc caaaccattt ccagcctgct catggcctct gcctcagtca
      121 tgaggcaatc tgttttacaa ggtttttctg gcaggtaaaa tataattttt atttaattat
      181 ctttgggtgg gaccgagatt ttggggtagc ctctgtggtc cggagtgatt tttttgttgt
      241 tataatctcg ccaatttgat aatttatggc accctattgc agtccccgcc attgactccg
      301 gatccgaaac aggagagtgc cttatcataa aagaatttat cgacatgccg cggctcgaga
      361 agtgggtggg ctggagcttt gcattcctct cgccgaacac ctggacgacc ctatcgctct
      421 tcgtcgcttt cgccgcctat attgctgtgc tttatgatcg cccctttctg ggcgccatca
      481 tcttcgccgt cagcgccctc atggatctcg tcgacggcaa ggtcgcgcgc tacaaaggca
      541 ccgcgaccag gctgggagcc ttctgggacg gcaccatcga ccgtttcgtc gatgccctga
      601 tgatcctctg cctcttcagc ctggagttcc ccccggccat cctgccggtc gggatcctgc
      661 ttttcctcct cctcttcttc accctgctgc cgcccttcat cgtcgcctat gccaatcatc
      721 gcggtgccgt gccggacccg accgaaaagg tgatctggcg cttcgccttc cgcatcgagt
      781 atatcgttct cttcatcgcg gccattctga tcaacggctg gtcgccgcac tggagccttt
      841 acctggtcta tgcggccctt gtgctcatgg tcgccacggt catccagtcg attattctcg
      901 tctttgtcaa gtccgggaac tatccgcagc agtgacgtcc agaccggcgc ggagggtggc
      961 gccgcagcac gcggaatccg ccctccggcg cgcgggatga agataatggt tgatatttaa
     1021 tcttccaggc cctaaatttt catcggcagc ctgagtccgg caccggcagc accctattct
     1081 ctccgaggcc agaattgtcg cgaaccgctt ccgttctact gctggtgatc tgcgcctgga
     1141 tggccccgct tacggcccag aacggcgcac tccagctcca tctcacgacc ctgatccagg
     1201 agcggctgaa gcagctcccc gaggagcgcc ggctggccac cgccgggggc agcgagaatc
     1261 tctccccgga tctcttcacc ctctatggcc ggcggggctt tcgcccgatc tgggcctccg
     1321 aatcgggcat ccgcgccgag gccgacagcc ttctcgccct catccgcaag gccgaccggc
     1381 acggcctcga tccggccagc tatcacgagc gggccctgaa cctgctgctg ccgcagctca
     1441 acagccgtct ccatgcggcc ccggtcgatc cggctgtact ggccgatctc gacctgctcc
     1501 tcagcgacgc ctatctggtg ctcggtctgc acctggccgc cggccgcttc aatccccatg
     1561 acctggacgc cgagtggtac ctgcacaaga acgaggcgga catggtgacg aggctgaacg
     1621 gggccgccga ggccggagag ctgctcgagg atctgcgccg cctgccgccg caggaggagg
     1681 agtatcacca cctgctcgag gcctacggct actaccgtca gctggccgtc gaggggggat
     1741 ggcccttcat cgctgatggc cgcaagctgg aaaagggaat gtgggatgaa cgggtgatgc
     1801 aggtgcgcgc ccgcctgcgg gcgaccgccg acctcgccct cgagccggcc gcggtcgagg
     1861 gcacctacga cgaggccctc gaacgggcgg tgcgccgctt ccagatgcgc cacggcctcg
     1921 aacccgacgg caaggtcggc aagagcaccc tggccgagat gaatgtgccg gtcgggaagc
     1981 gcatccgcca gctcgccgtc aatctcgaac gctggcgctg gctgccgcgc gaccacggcc
     2041 gccgctatat acgcgtcaac atcgccgact ataccctcca gctctttgag gccgacacgg
     2101 tggccatggc gatgcgcgtg gtggtgggca agacctatcg ccgcaccccg atcttcagcg
     2161 acctgatgac ctatctggtc atcaatccct actggacggt cccgaatacg attctgctca
     2221 acgatatcgt ccccaaggcg ctgaagaatc cggactatat tcacaaggag cggatcaaga
     2281 tctacgccgg ctggagtgaa aacgcccccg agatcgatcc cgcctcggtc gactggaaca
     2341 ggctctccaa gaactatcag ccttaccgcc tgcgccagga tccgggtccg ctcaactcc
//
LOCUS       DAOPWU010000468         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_46939_length_2389_cov_2.844045, whole genome shotgun sequence.
ACCESSION   DAOPWU010000468 DAOPWU010000000
VERSION     DAOPWU010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46939_length_2389_cov_2.844045"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PKM23_15240"
     CDS             complement(<1..173)
                     /locus_tag="PKM23_15240"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926764.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNY92877.1"
                     /translation="MSELKEVVIIDAVRTPIGSFGGALAEIPAPKLGSTVIKALLQRT
                     AIAPDQIDEVIMG"
     gene            complement(201..713)
                     /locus_tag="PKM23_15245"
     CDS             complement(201..713)
                     /locus_tag="PKM23_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNY92878.1"
                     /translation="MLKQQFIQDFGAGYQMFGHPALMGRIVGLLLYHGKPVSLTDITK
                     ELGVSKGPASQICRRLLDHNLIQRVWKPGSRKDYYQAHPNIFGNAFMNHLALQRKNLE
                     LAQKFQSALLESGTEESAAFSKNMEEMVAFYQLMIERYLAFYRDWMTQKEGTLTVPAA
                     LQQDAAGARG"
     gene            1030..>2389
                     /locus_tag="PKM23_15250"
     CDS             1030..>2389
                     /locus_tag="PKM23_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNY92879.1"
                     /translation="MHPSLQKYHFSLRLKITLLFLMLVIVMMATIGYLFTVRELDLRK
                     EQMKVRMERLANNIAAIRSVETEDWDVYQTYIDNQIKVNPDIVYIAIFDDQNRLRVHA
                     LNRDWLELESSRPLTRMEQAGIIMQLDQRQVAAESQRDFERQSVQIMVGVRNLGRVNI
                     GFSLVDLNDEMKRNLQSNLLIDSVFLLLAVLLALFIGSRIINPLGKLTAAMAGIAQGK
                     HGQQLQITSRDEIGKMASTFNYMSRGLQEKQIIENYSRELGVTIELEKIARFITGRIG
                     AALRARAGLLFLGGREEAGRFGLVSAWPEGPPAGARLEIGEESIALLLEGCSARPLTT
                     LPDHAALRSVLLAECESKGEALVAPVVIKEALRGIFILCGPQEPPDYSEDEIRLLDTL
                     LSQGGIAIENALLYEELAGQERLKQEFEIARRVQQSLLPQETPRMPGLEIDAICIPAE
                     EIG"
BASE COUNT          479 a          667 c          744 g          499 t
ORIGIN      
        1 cagcccatga tcacttcgtc gatctgatcc ggggcgatgg cggtgcgctg cagcagggcc
       61 ttgatgaccg tgctgccgag tttgggggcg gggatttcgg cgagggcgcc gccgaagctg
      121 ccgatcggcg tgcggacggc atcgataatg accacttcct tgagctcaga catgggatgt
      181 cctttgatag agttcggttt tcagccgcgg gcgccggcag catcctgctg cagcgcagcc
      241 ggcacggtta gggttccttc tttctgtgtc atccagtcac ggtaaaaggc cagatagcgt
      301 tcgatcatca gctggtaaaa ggcgaccatc tcctccatat tcttgctgaa ggccgcgctc
      361 tcctcagtac cggactccag cagggcgctc tggaatttct gcgccagttc aagattttta
      421 cgctgcaagg cgagatggtt catgaaggcg ttgccgaaga tattggggtg ggcctggtaa
      481 tagtccttgc ggctgcccgg tttccagacg cgctgaatga gattgtgatc gaggagtctt
      541 ctgcagattt gcgaggccgg gcctttgctg accccgagtt ctttggtgat atcggtgagg
      601 gaaacaggtt tgccatggta gaggagaaga ccgacgatgc ggcccatgag ggcgggatgg
      661 ccgaacatct ggtagccagc gccgaaatcc tggatgaatt gttgtttcag catgccgcac
      721 cttctgtttt aatcgtttga aatatttcaa acgattaaaa ttagacaatc ttttgtgtaa
      781 aagcaagtac tttattaccg aaaatcgatg tcacgcggcg ccagagccga aggtgttccg
      841 gcgaggccgg gacggacccg ccattgccaa gagggagagc gccgcatcgt cccaggtttc
      901 gataaaagcg cttgtttatc gtactccgga tagctaattt ggagtaacct gttttccccc
      961 gatatcaggg ggcgaaactt tatcatcggc gtgctgcgta ttaaaggtgt acccgctcgg
     1021 aaaagaacca tgcatccgtc attgcaaaaa tatcacttca gcctcagact caagatcacc
     1081 ctccttttcc tgatgctggt catcgtcatg atggccacca tcggctacct ctttaccgtg
     1141 cgggagcttg acctgcgcaa ggagcagatg aaggtgagga tggagcggct ggcgaacaac
     1201 atcgccgcga tccgttccgt cgagaccgag gactgggatg tctaccagac ctacatcgac
     1261 aaccagatca aggtcaatcc tgacatcgtc tatatcgcca tcttcgatga ccagaaccgg
     1321 ctgcgggtgc acgccctcaa ccgggattgg cttgagctgg agagcagccg tcccctgacg
     1381 cggatggagc aggccgggat catcatgcag ctggaccagc gacaggttgc ggccgagagc
     1441 cagcgcgatt ttgaacggca gtcggtccag atcatggtcg gagtccgcaa tctgggcagg
     1501 gtcaacatcg gtttctccct ggttgatctg aacgatgaga tgaagcgcaa tctccagagc
     1561 aatctcctca tcgattctgt ttttctgctc ctcgcggtgc tgctcgctct cttcatcggc
     1621 tcacgcatca tcaatcccct cggcaagctg accgcggcca tggcggggat tgcgcaggga
     1681 aagcacgggc agcagctgca gatcacctcc cgcgacgaga tcggcaagat ggcctccacc
     1741 ttcaactata tgtcccgcgg gctgcaggag aagcagatca tcgaaaacta ttcccgggag
     1801 ctgggtgtca ccatcgagct ggaaaagatc gcccggttca tcaccggccg cataggtgca
     1861 gcgctgcggg ccagggcggg gctgcttttc ctcggcggcc gggaggaggc gggccgcttc
     1921 gggctggtga gcgcatggcc ggagggtccg ccggccggag cgcgcctcga gatcggggag
     1981 gagagcatcg ccctcttgct cgaggggtgc tctgcacggc ccttgaccac cctgccggac
     2041 catgcggctc tccggagtgt cctgctggcg gaatgtgaat ccaaagggga ggcgctggtc
     2101 gcgccggtgg tcatcaagga ggcgttgcgc ggaattttca tcctttgcgg tccgcaggag
     2161 ccgccggact actcggagga cgagatccgg ctgctggaca ccctcctttc tcaggggggg
     2221 atcgccatcg agaacgccct gctctacgag gagcttgccg gacaggagcg gctcaagcag
     2281 gaattcgaga tcgcccggcg ggtgcagcag agtctgctgc cgcaggagac cccgcgcatg
     2341 cccgggctgg agatcgacgc gatctgcatc cccgcagagg agatcggcg
//
LOCUS       DAOPWU010000469         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_46973_length_2387_cov_4.246141, whole genome shotgun sequence.
ACCESSION   DAOPWU010000469 DAOPWU010000000
VERSION     DAOPWU010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46973_length_2387_cov_4.246141"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PKM23_15255"
     CDS             complement(<1..733)
                     /locus_tag="PKM23_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92880.1"
                     /translation="MKSNYLRLATLLSLLLHLGLGLFFYFWRLNQAAPTAGEAADVAF
                     IELPEPLPAEPVLPESRESSEELVTERPTPGPVEEAPILAPELPVSAAAGEETITWPA
                     HLDSLLAIADSVARTRQLSSNSVPEFFPAPIPLVPYREERRKKRPTLFPLDRMTGHPG
                     AYTDRAAEEQYRRGTGGGQQPVDLTALAAAGVKALGKALGKEKVPPPPRLRKVPTREE
                     MAALCVIWTSEEPTEYDIYHDLDADI"
     gene            921..1256
                     /gene="cutA"
                     /locus_tag="PKM23_15260"
     CDS             921..1256
                     /gene="cutA"
                     /locus_tag="PKM23_15260"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNY92881.1"
                     /translation="MESFLLVTTTCASREEAEVLAEKLLKVRLIACANIFSGVHSIFH
                     WRGKIERAEEVVLQVKCRKRYFRDLVEWIQTHHSYELPEILALPIVDGSREYLDWMME
                     ETRFTEFDN"
     gene            1266..2372
                     /locus_tag="PKM23_15265"
     CDS             1266..2372
                     /locus_tag="PKM23_15265"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNY92882.1"
                     /translation="MRRTKHPLATRVTNLEPSGIRKVFEIANAHPEYINLSIGEPDFD
                     MPMFLKEQGIKAILAGRNRYTPTRGIPELCRLIADQLTAEGIRFGDLFVTAGATGGLL
                     LSIQALVGPGDEVIVPDPYFVAYRNLVYMAGGTPVGADTYPDFQIKAGAVESLITERT
                     RALILNSPNNPTGAIYERAELEKLVALADHHGFHIISDEVYTPFIYEGNYTSLGLLTD
                     DAIIVNSFSKSAAMTGWRLGYVSGPAGVIDRMAMLQQYAYASTNSVAQYTALRAFEVD
                     YSEQRGNYRRKRDLCYDMLSTRYELHKSRGSFYFFPKAPDGNATAFFERARAAGVLII
                     PGKDFSRRDTHFRISFAVADDLLEQGMEILLHLA"
BASE COUNT          465 a          736 c          694 g          492 t
ORIGIN      
        1 tgatatccgc gtccaggtcg tggtagatgt cgtattccgt cggctcttca ctggtccaga
       61 tcacgcagag ggccgccatc tcctcccggg ttggcacctt gcggaggcgc ggcgggggcg
      121 gaaccttctc cttgccgagg gccttgccga gggccttcac tcctgcggcg gccagggctg
      181 taagatcaac gggctgttgg cccccgccgg ttccgcggcg gtactgctcc tctgcggccc
      241 ggtcggtata ggcgcccgga tggccggtca tgcggtcgag gggaaaaagg gtcggccgct
      301 ttttcctgcg ttcctcacgg taggggacaa gggggatcgg ggcggggaaa aactcgggca
      361 cggagttgga actcagctgc cgggtgcgtg caacgctgtc ggcgatcgcc agcaggctgt
      421 ccagatgagc gggccaggtg atggtctcct cccccgccgc cgcagagacc ggcagctccg
      481 gcgccaggat gggggcttcc tcaaccggtc cgggcgtcgg gcgctccgtc accagttcct
      541 cgctgctttc acgggattcg gggagaacag gttcggcggg aaggggttcg ggcagctcga
      601 taaaggccac atcggcggcc tcgccggccg ttggcgcagc ctgattcagg cgccaaaagt
      661 aaaaaaagag gccgaggccg agatgaagca gcagcgagag cagcgtggcc agccgcaggt
      721 aattggactt catatatgat aaaccatagc gtggactctt aattagagcg cttcctgcct
      781 gaattgcacc aggatctccg ttgatcgagc agggattgtt ttgttgatac gccctcccgg
      841 ggtcaaagtt cctcggatac ctggcggcgc tataatgtac agaacagaaa tttcttatca
      901 aaccatttta gcggggccct atggaatctt ttcttctggt aacgactacc tgtgcctccc
      961 gtgaggaggc cgaagtgctg gcggaaaaac tgctcaaggt gcggctgatc gcctgcgcga
     1021 acatcttttc cggcgtgcat tccatttttc attggcgggg caagatcgaa cgcgccgaag
     1081 aggtggtgct gcaggtgaaa tgccgcaaac gttatttcag ggatctggtc gaatggattc
     1141 agacccatca ttcctacgag cttcctgaaa tcctcgccct tcccatcgtc gatggatcca
     1201 gggaatatct ggactggatg atggaggaga cccgttttac tgaattcgac aattaggatg
     1261 accgcatgag aaggacgaag caccccctgg cgactcgcgt caccaatctg gagccgtcgg
     1321 ggatccgcaa ggttttcgaa atcgccaacg cccatccgga gtacatcaac ctctcgatcg
     1381 gcgaacccga ctttgacatg ccgatgtttc tcaaggaaca ggggatcaag gcaatcctcg
     1441 ccggccgcaa ccgctatacc ccgacgcgcg gcatcccgga actctgccgg ctcatcgccg
     1501 accagctcac agcggagggg atccgtttcg gggacctttt tgttaccgcc ggggccaccg
     1561 gcggactcct gctcagcatc caggccctgg tcggcccggg tgacgaggtg atcgtgccag
     1621 atccctattt tgtcgcctac cgcaacctcg tctacatggc cggcggcacc cccgtcggtg
     1681 ctgataccta tcccgatttc cagatcaagg ccggcgcagt ggaatccctt atcaccgagc
     1741 gcacccgtgc cctgatcctc aacagcccca ataatcccac cggtgccatc tatgaacgcg
     1801 ccgagctcga aaaactcgtc gccctcgccg atcaccacgg ctttcatatc atttccgatg
     1861 aggtctatac cccattcatc tatgaaggga actataccag cctcggcctg ctgacggatg
     1921 atgccatcat cgtcaacagc ttttccaaga gcgccgccat gacgggctgg cggctcggct
     1981 atgtctccgg cccggccggg gtgatcgacc gcatggccat gcttcagcag tacgcatacg
     2041 ccagcaccaa ctcggtcgcc caatacaccg ccctgcgcgc ctttgaagtc gattatagcg
     2101 agcagcgcgg caattaccgg cgcaagcgcg atctctgcta tgatatgctc tccacccgct
     2161 acgaactgca taaatcccgg ggcagtttct atttttttcc caaggccccg gacggtaatg
     2221 cgactgcctt tttcgaacgg gcgcgggcgg ccggcgtcct tatcatcccc ggcaaggatt
     2281 tctcccggcg ggatactcat tttcgcatct ccttcgccgt cgccgacgac cttctcgagc
     2341 agggtatgga aatcctcctc cacctagcct gaaaggaaac attttgg
//
LOCUS       DAOPWU010000470         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_47174_length_2380_cov_5.069677, whole genome shotgun sequence.
ACCESSION   DAOPWU010000470 DAOPWU010000000
VERSION     DAOPWU010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47174_length_2380_cov_5.069677"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /gene="uvrC"
                     /locus_tag="PKM23_15270"
     CDS             complement(<1..1032)
                     /gene="uvrC"
                     /locus_tag="PKM23_15270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNY92883.1"
                     /translation="MPKNVLEKLDLLAKKPGVYLFKNRRGEVIYVGKAKVLRNRVRSY
                     FQEHRPLDAKTAKLVSHIADFETIITDSEVEALILESNLVKEYKPRYNINLKDDKSFP
                     YIRVTNETFPRIFPTRKIIRDGSRYFGPYTDVYPMRELLKTVKQLFPLRSCNLALTEE
                     SIRQHKFRVCLNYHIKKCHGPCEGHVSCEEYQKTIDFIVAFIEGKSTAVVEAMTREMH
                     AAAADRRFEEAARLRDQLLAVQNFHQRQKVLDPALRDRDIIAIATEDGDACCVVFQVR
                     EGKIIGRQHFFLNGIAEEDLSAVTAAFVKQYYITREYVPLEILLPAPLGDEAAPIRSW
                     LSDKRNGAVD"
     gene            complement(1086..>2380)
                     /locus_tag="PKM23_15275"
     CDS             complement(1086..>2380)
                     /locus_tag="PKM23_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNY92884.1"
                     /translation="EVLATKICTHFNRMKLETRTDGVEELVGNIKSNVLMIGPTGVGK
                     TYLIKLIAKKIGVPFVKGDATKFSETGYVGGDVEELVRDLVREANGDLKLAEYGIIYL
                     DEIDKIASSGNSIGPDVSRSGVQRNLLKLMEESEVDLKTPHDLAAQMEAAMEAQRTGK
                     VARKKVNTRNILFVMSGAFSTLPDIIRRRLNQQPIGFRGVEEELPASAADTEILRKLR
                     SEDLIRFGFESEFVGRLPVTVTLNDLDVEGLFRILGNPNSTVIQGKRRDFRSYDIHIE
                     FEEEALRRIAELANEEHTGARGLVSVVDRLLLKFEKTLPDTTITEFTVTPALVDNPQG
                     ELDRLLTQHYLKAFQKRFLASNGIVITFTGEALELLRQRASGEGKNLEEVCSDLLHDY
                     EYGLRLLGCDNFTVDSDIVRDPKSRLEEMIKQSYGKKH"
BASE COUNT          467 a          632 c          723 g          558 t
ORIGIN      
        1 atccactgcg ccattacgct tgtccgacag ccagctcctg atcggggccg cctcatcccc
       61 cagcggggcc ggcagaagaa tctcgagcgg cacgtactcg cgggtgatat aatactgctt
      121 gacaaaggcg gcggtgacgg ccgaaagatc ttcctcagcg ataccgttga gaaaaaagtg
      181 ctggcgtccg atgatcttgc cctcgcgcac ctgaaagacg acgcagcagg catcgccgtc
      241 ctcggttgcg atcgcgatga tgtcccggtc acgcaaggcg ggatcgagca ccttctggcg
      301 ctggtgaaag ttctgcactg caagcagctg gtcgcgcagg cgtgccgctt cctcgaaacg
      361 gcggtcggct gcggcggcgt gcatctcccg ggtcatggcc tcgaccacgg ctgtgctttt
      421 accttctatg aaagccacga tgaaatcaat ggttttttgg tattcttcac atgaaacatg
      481 accctcacag ggaccatgac atttcttgat gtgatagttg aggcaaacgc ggaatttgtg
      541 ctgtcgaatg ctctcttcgg tcagagcgag gttacatgag cgcaaaggga agagctgctt
      601 gacggtcttg agcagttcgc gcatcggata gacgtcggta taagggccga aatagcgcga
      661 gccgtcgcgg atgatcttgc gggttggaaa gatgcgcggg aaggtctcgt tggtgacgcg
      721 tatatacggg aaacttttat cgtccttgag attgatgttg tagcggggtt tgtactcctt
      781 gaccaggttg gattcgagga tgagggcttc cacctccgaa tcggtgatga tggtctcaaa
      841 gtcggcgata tggctgacca gtttggcggt tttggcgtcg agaggacggt gttcctggaa
      901 ataggagcgc acccggttgc gcagcacctt ggccttgccg acgtagatga cttctccgcg
      961 cctgttcttg aacagataga cgcctggctt tttagcgagc aagtccagtt tttcgagaac
     1021 gttctttggc acggaatcgg tcatagtcat ccgtatgctt ggcaaccggg tcgctggtga
     1081 aggtactagt gcttcttgcc gtaggactgt tttatcatct cctccagccg cgacttggga
     1141 tctcggacga tatcgctgtc cacggtaaaa ttgtcgcagc cgagcaggcg caagccatat
     1201 tcataatcgt gcagcagatc gctgcacacc tcttccagat ttttgccctc cccggatgct
     1261 ctttgcctga gcagttcaag cgcctcgccg gtgaaggtga tgacgatgcc gttgctggcg
     1321 aggaagcgtt tctggaaggc cttgaggtag tgttgggtga gaaggcgatc cagttctccc
     1381 tgcggattgt ccaccagggc aggggtgacg gtgaactcgg tgatggtagt gtccgggagt
     1441 gttttctcga atttgagcaa gagacggtcg accacgctca cgaggccgcg cgcacctgta
     1501 tgttcctcgt tggccagctc cgcaatgcgg cgcaaggcct cctcctcaaa ttcgatatgg
     1561 atatcatagg agcggaagtc ccgcctttta ccttgaataa ccgtactgtt gggatttccg
     1621 aggatgcgga agagaccctc gacatcgaga tcattgaggg tcacggttac cggaaggcgg
     1681 ccgacgaatt cggattcaaa gccgaagcgg atgaggtctt ctgaacgcag cttgcgcagg
     1741 atttccgtgt ccgctgctga agcaggcagc tcctcctcca cgccccggaa gccgatcggc
     1801 tgctggttga ggcggcggcg aatgatatcc ggcagggtcg aaaaggcccc gctcataacg
     1861 aagaggatgt tgcgggtatt gaccttttta cgcgcgacct tgccggtgcg ctgggcctcc
     1921 atggccgcct ccatctgggc ggcgagatcg tgaggggtct tgaggtcgac ctcggactct
     1981 tccatcagtt tgagcaggtt gcgctgcacc ccggagcgag agacatcggg gccgatgctg
     2041 ttgccggaag aggcaatctt gtcgatttca tccagataga taatgccgta ttcggccagc
     2101 ttgagatccc cattggcttc gcggaccagg tcgcgtacca gctcctcgac atctccgccg
     2161 acatagccgg tctcgctgaa tttggtggca tcgcccttga cgaagggcac gccgatcttt
     2221 ttagcgatca gtttgatcag ataggtcttg ccgacgccgg tggggccgat catcagcacg
     2281 ttgcttttaa tatttccgac cagctcctcc accccgtcgg tgcgggtttc cagcttcatg
     2341 cgattgaagt gggtgcagat cttggtggcg agcacctcaa
//
LOCUS       DAOPWU010000471         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_47181_length_2380_cov_3.742796, whole genome shotgun sequence.
ACCESSION   DAOPWU010000471 DAOPWU010000000
VERSION     DAOPWU010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47181_length_2380_cov_3.742796"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..1808)
                     /locus_tag="PKM23_15280"
     CDS             complement(282..1808)
                     /locus_tag="PKM23_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter TolR"
                     /protein_id="HNY92885.1"
                     /translation="MIIPLRTIVSLAGALLLVCMTPLSLNAQIPHSRIGIFDGFSDIG
                     SPGIPGSVSCHEPGQMVRLSGSGANIWFARDSFSFLWKQMNGDFILQTRLSWISTGHE
                     PHRKTGIMIRESQRASAAMVACTVHGDGLTCLQYRKAGDEDVQEIRFSVTGPDVVQLE
                     KKGNLFTMSAARFGDRYEAQNLENVNLPDNLLAGLFICSHNDTISEEVEFTNVRIFNT
                     APDTLVQYRDYLGSLLEILEVESGKRHVLAGSDHSWQAPNWTPDGSALIYNAEGLLYQ
                     SDIRTGVSSLLNTGFADRNNNDHVLSFDGRLIGISHHAKESGGESVVYTLPAAGGTPT
                     RVTAKSPSYLHGWSPDSRFLVYTGERDGEFDIYKIPAEGGREIRLTTSRGLDDGSEYA
                     PDGRSIFFCSSRTGKMQIWRMDPDGKNQQQLTFDDLNNWFPHVSPDNKWIAFLSFPQE
                     VPADQHPFYQRVYLRLMPATGGEPRVIAYLYGGQGSINVPSWSPDSRRIGFVSNGQFR
                     "
     gene            complement(1941..>2380)
                     /locus_tag="PKM23_15285"
     CDS             complement(1941..>2380)
                     /locus_tag="PKM23_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92886.1"
                     /translation="PNMGAWTSELLITRPLGNAHLITFKAGISLCLFRSSALDHRTTI
                     DLPLVFPRLQPFYQEYTWRSGIEGMGRLGRRWLYLVDGDLFYTPGGEENWALEHKGML
                     LWDKSSRAQLGLGYKLTWGEYPFGTQWHLLGPLIDLQWGWGGR"
BASE COUNT          483 a          685 c          704 g          508 t
ORIGIN      
        1 aggaagccag tcccaacaac atttctgtcc tgccccgttt cgcttcggcc catcctgccg
       61 gcgcgaggga ggcggctgcc gccaccacac cggcggtttt gaagaattct cgacgattct
      121 gcgctctgga catgaatggc ctcacttgtt tgattggatc tactggtcaa aatttgttcg
      181 agctggccgc gctgcgccgg aaagccggcc gtgctgcatc gggaagccgg tggcagactg
      241 tcttctggca aaaccctggc aatcgatatc agcgtcaacg atcatctgaa ttgcccgttg
      301 ctgacgaaac ctatcctgcg actgtccggg gaccagctcg gaacgttgat cgatccctgg
      361 ccgccgtaga gataggcgat cactctgggt tccccgcctg tcgcgggcat caggcgcaaa
      421 tagacccgct gataaaaggg atgctggtcc gcagggacct cctgcgggaa tgaaagaaag
      481 gctatccact tgttgtcagg cgagacgtgc ggaaaccagt tgttcagatc atcaaaagtc
      541 agctgctgct gattttttcc gtccggatcc atccgccaga tctgcatctt gccggtgcgg
      601 ctggagcaaa aaaagatgga cctgccatcg ggagcatact ctgatccgtc gtcgaggccc
      661 cgggatgtcg tcagccggat ttcacggccg ccctcggccg ggattttgta aatatcgaat
      721 tcaccgtccc gctcaccggt atagaccaga aatctgctgt cgggggacca cccgtgcagg
      781 tatgacgggc ttttcgcggt gaccctcgtg ggcgtgcccc cggcagcggg cagggtgtag
      841 accacggatt ctcccccgct ctcttttgca tgatggctga tgccgataag ccgcccgtcg
      901 aagctcagga catggtcgtt gttgttccgg tcagcaaaac cggtgttcag gagcgatgag
      961 accccggtcc ggatgtcgga ctggtagagg agcccctccg cattgtagat cagagcgctg
     1021 ccgtccgggg tccagttcgg ggcctgccag gagtggtctg atccggccag cacatgcctc
     1081 tttccgctct cgacctccag gatctcaaga agactgccca gatagtcccg gtattgcacc
     1141 agcgtatccg gggccgtatt gaagatccgg acattggtga attcaacctc ctcggaaatg
     1201 gtgtcgttgt gggaacagat gaaaaggccg gccagcagat tatccgggag gttcacattc
     1261 tcgaggttct gcgcctcata cctgtcgccg aaccgtgcgg ccgacatcgt gaagagattg
     1321 cccttctttt cgagctgcac tacatccgga ccggttacgg aaaatctgat ctcctggaca
     1381 tcttcgtccc cggccttcct atactgcagg caggtcaggc cgtccccgtg aacggtgcag
     1441 gcgaccatgg cagcgctggc tcgctgcgac tcccggatca tgatgccggt cttgcgatgg
     1501 ggttcatggc cggtgcttat ccatgagagc cgtgtttgca ggatgaaatc gccattcatc
     1561 tgcttccaga ggaaagaaaa gctgtccctg gcgaaccaga tattggcgcc ggaaccggag
     1621 agtcggacca tttgtccagg ttcatggcag ctgacggagc ccgggatgcc gggtgagcca
     1681 atgtccgaaa agccatcaaa tattccaatc ctggaatgag gtatctgtgc attgagggag
     1741 agcggcgtca tgcagaccaa tagcagtgcg ccggcaaggc tgacgatggt gcggaggggt
     1801 atgatcatcc tggttttcct tcttcgacgg gataaatgcc tttccttatc caattgagtc
     1861 actgcttcat tccgataaac gccgcgagcg cactcttgcg gcaaatgagc caggcagctg
     1921 tcaaaataca caggtggctt tcagcgcccg ccccagcccc actgcagatc gatgaggggc
     1981 ccgagcagat gccactgcgt gccaaaggga tattcccccc aggtgagttt atacccgagt
     2041 cccagctgcg ccctgctgct tttgtcccac agcagcatgc ccttgtgttc tagcgcccag
     2101 ttttcctctc ccccgggggt gtaaaagaga tcgccgtcca ccaggtagag ccagcggcgg
     2161 ccgaggcggc ccatcccttc gatgccgctg cgccaggtat attcctgata aaacggctgg
     2221 agccgcggga agaccagcgg cagatcaatg gtcgtccgat ggtcgagtgc gctggagcga
     2281 aagagacaca gactgatccc ggctttaaaa gtgatgagat gggcgttgcc gagggggcga
     2341 gtgatcagta attcactggt ccatgcgccc atgttgggga
//
LOCUS       DAOPWU010000472         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_47619_length_2366_cov_3.644743, whole genome shotgun sequence.
ACCESSION   DAOPWU010000472 DAOPWU010000000
VERSION     DAOPWU010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47619_length_2366_cov_3.644743"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="PKM23_15290"
     CDS             complement(<1..1091)
                     /locus_tag="PKM23_15290"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNY92887.1"
                     /translation="MSYRSAILLLALLALCAGLLPAPLMAGETGKISGRVTDAANGQP
                     LIGANVMIVERIESGRVIPLSGSAVQGAVTDPNGEYYIINIKPGVYTVRFVYIGYQSV
                     NKTQVAVSVDLTTKLDTRLSTTVLESGESIDIIADRVQIQKDLTSSQVSFGADKIDQL
                     PVRSVAELVNLQAGVVTDDGGNLHIRGGRTSEITYMVDGVKVVDPLSRSAGISIDDQA
                     IQELKTITGTFNAEYGQALSGVVNIVTKQGSDEFRINATGYFGDHLSFDDGTYYVMNN
                     PAWANAAARALTRGGNFILYDFGDVNPYTSSAQATKPWLTKKSYLDSYNPTTNTDFQV
                     NISGPIPFTQKRLTYFISGRVQDSPSFTQG"
     gene            complement(1280..>2366)
                     /locus_tag="PKM23_15295"
     CDS             complement(1280..>2366)
                     /locus_tag="PKM23_15295"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNY92888.1"
                     /translation="AAVDAEGTEYDLGDKSRVGPLTCNSSVTAKAFYVENFASVFSLD
                     GLDNEFTDYKQYGLKAERHNGSDTTGWTPASTDMSWNATFIIDANYLYISADVTDDDL
                     NASGSEPIVSGSQAWMGDALEFYLGFYNSVLEPPHLYKDVDLAGTGDWRISFTAWGTT
                     QKSGSSDYEFPGVERTVYQKFTGDGYIIEVRIALDSLAKGGNLDVVDGAMLPLRIDGT
                     DLDPSKGDTERTLWTGWGNQANSEDWKRPSTFGFLQVVGGPTAVEAEAQQPLVFELKN
                     NYPNPFNPVTTINYQVARSTQVRIAVYDLLGKEVATLVDGRMAAGAHVITWNGQNNAG
                     QAVSSGVYFCRMVTPEYTHILKMTLLK"
BASE COUNT          461 a          632 c          796 g          477 t
ORIGIN      
        1 ccctgggtga agctcggact gtcctgcacg cgccccgaga tgaaataggt gagccgcttc
       61 tgggtgaaag ggatcggtcc cgagatgttg acctggaaat cggtgttggt ggtcgggttg
      121 tagctgtcga gataggattt cttcgtcagc cagggtttgg tcgcctgggc ggaggaggtg
      181 tagggattga cgtcgccgaa atcgtagagg ataaaattgc ccccgcgggt gagagcacgc
      241 gcggcggcgt tggcccaggc agggttgttc atgacgtaat aggtgccatc atcgaagctg
      301 agatggtctc cgaaatagcc ggtggcattg atacggaatt catcgctgcc ctgcttggtg
      361 acgatgttga ccacgcccga gagggcctgg ccatattcgg cgttgaaggt gccggtgatg
      421 gtcttgagct cctggatggc ctgatcgtcg atgctgatgc ccgccgagcg gctgagggga
      481 tcgaccacct tgacgccgtc gaccatgtag gtgatctcgc tggtgcgacc gccgcggatg
      541 tggaggttgc cgccatcatc ggtgactacg ccggcctgca gattgaccag ttcggcgacg
      601 ctgcgcaccg gcagctggtc gatcttgtcc gccccgaagg agacctggga ggaggttaaa
      661 tccttctgaa tctgcacccg gtcggcgatg atgtcgatcg attccccgct ctcgagcacc
      721 gtggtggaga gacgggtgtc gagtttggtg gtgaggtcga cgctgaccgc cacctgggtc
      781 ttgttgaccg actggtagcc gatatagacg aagcggacgg tgtagacccc gggcttgatg
      841 ttgatgatgt aatactcgcc gttgggatcg gtgacagccc cctgaacggc cgatccgctg
      901 agcggaataa cccgcccgct ttcgatgcgt tcgacgatca tcacgttggc gccgatgagg
      961 ggctgcccgt tcgcggcatc ggtgacccgg ccggagattt ttcccgtctc ccccgccata
     1021 agcggcgccg gtagcagacc ggcgcacaag gccaggaggg cgagcaggag gatagcggag
     1081 cggtacgaca tagtgcctcc aacaaattag ggcattgcac tgcatgggtg agaaaaggac
     1141 agtaggatct gccggaagac aggggtgatg aagatttccg ctgtcctctg gcagagcccc
     1201 tctcctcgcg gccggggggc tgccggttga cgttaaaaaa ggatggggtg atcacaagac
     1261 caccccatcc ggtgaggaat tacttgagaa gcgtcatctt gagaatatgg gtgtactccg
     1321 gcgtgaccat gcggcagaaa tagacgccgc tgctgacggc ctgaccggcg ttgttctgac
     1381 cattccaggt gatgacatgc gcgccggcgg ccatgcggcc gtcgaccagg gtcgccacct
     1441 ctttgccgag cagatcatag accgcaatcc ggacctgggt gctgcgggcc acctgatagt
     1501 tgatcgtggt gaccgggttg aaggggttcg gatagttgtt cttgagctcg aaaaccaggg
     1561 gctgctgggc ttcggcctcg acagcagtcg ggccgccgac cacctgcagg aaaccgaagg
     1621 tggagggacg cttccaatcc tcggaattgg cctggttgcc ccaaccggtc cagagcgtac
     1681 gctcggtgtc gcccttggag ggatcgagat cggtgccgtc gatgcgcagg ggcagcatgg
     1741 cgccatcgac gacatcgaga ttgccaccct tggccagaga atcgagggcg atgcggactt
     1801 cgatgatgta gccatcgccg gtgaatttct ggtagacggt gcgctccact cccgggaact
     1861 cataatcgga ggagccgctc ttctgcgtgg tgccccaggc ggtgaagctg atgcgccaat
     1921 cgccggtgcc agcgagatcg acatccttgt agagatgggg cggttcgagc acggaattgt
     1981 aaaagccgag atagaactcg agggcatcgc ccatccaggc ctggctgccg gagacgatcg
     2041 gctcgctgcc ggaagcattg aggtcatcat cagtgacgtc agcggagatg tagagataat
     2101 tggcatcaat gatgaaggtg gcgttccagc tcatatcagt ggaagccggg gtccagccgg
     2161 tggtgtccga accgttgtga cgctcggcct tgaggccata ctgcttatag tcggtgaact
     2221 cattgtccag gccgtccagg gagaagacgc tggcaaaatt ctcgacatag aaggccttgg
     2281 cagtgacgct gctgttgcaa gtgagtgggc cgacccgcga tttgtcaccg agatcatatt
     2341 cagttccctc ggcatcgacc gccgcg
//
LOCUS       DAOPWU010000473         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_48562_length_2334_cov_4.350592, whole genome shotgun sequence.
ACCESSION   DAOPWU010000473 DAOPWU010000000
VERSION     DAOPWU010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48562_length_2334_cov_4.350592"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PKM23_15300"
     CDS             <1..949
                     /locus_tag="PKM23_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNY92889.1"
                     /translation="ETVGDDIAWMKFGEDGRLYAINPEFGFFGVAPGTSMESNRNAML
                     SMRENSIFTNVALTPEGDVWWEGMTPEKPPRLTSWLHEPWTPDGGKPAAHPNARFTAP
                     AGQCPAIDPAWEDPRGVPISAILFGGRRPSLVPLIHQSFNWQHGVFLGSVVSSERTAA
                     ADGTVGAIRRDPFAMLPFCGYNMADYFAHWLCLGNRHDADKLPRIFYVNWFRKNAAGR
                     FLWPGYGENSRVLKWVFERCSGAGEALTTPIGHVPAPGALDVRGLEIGEAELAELFRV
                     DREGWIREAASIREYYATFGPRFPQQLYEELGALEGRLR"
     gene            1136..1978
                     /locus_tag="PKM23_15305"
     CDS             1136..1978
                     /locus_tag="PKM23_15305"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNY92890.1"
                     /translation="MREIAAPIISEQVARLCIDANYYLGADVLSALEEALAKEESPTG
                     RAILEQIIENAGIAREGEFPMCQDTGFAVFFVELGQEVVVTGQTLEEAITEGVRKGYG
                     EGYLRKSIVSDPLQRVNTKDNTPPVIWIETKPGRELKITIAPKGGGSENMSEVAMLTP
                     SDGAEGIKNFVVDKVRRAGSNPCPPIVVGVGIGGTFEKVAWLAKKSLLRPVGDRNPDP
                     FYAAMELELLERVNKLGIGPQGLGGRTTALDVHVEVFPCHIASLPAAVNIQCHAARHK
                     SVVI"
     gene            2013..>2334
                     /locus_tag="PKM23_15310"
     CDS             2013..>2334
                     /locus_tag="PKM23_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HNY92891.1"
                     /translation="MAEAIRITTPLTDDVVKKLRAGDSVFITGIIYTGRDAAHKRMVD
                     LLDKGEPLPVDIAGHLIYYVGPSPARPGKPIGSAGPTTSGRMNAYAPRLLAIGSKGMI
                     GKGEM"
BASE COUNT          430 a          760 c          724 g          420 t
ORIGIN      
        1 ggagacggtc ggcgacgata ttgcctggat gaagttcggc gaggacggcc gcctttatgc
       61 gatcaacccc gaatttggtt ttttcggggt ggcgcccggc acctcgatgg agtccaatcg
      121 caacgccatg ctgagcatgc gcgagaactc gatcttcacc aatgtggccc tcaccccgga
      181 aggggatgtc tggtgggagg ggatgacccc ggaaaagccg ccccgtctca ccagttggct
      241 ccatgagccc tggacgcccg acggcggcaa gccggcggcc catcccaacg cccgcttcac
      301 cgccccggcc ggccagtgtc cggccatcga tccggcctgg gaggatcccc gcggtgtgcc
      361 catctccgcc atcctcttcg gcgggcgccg gccttcactg gtccctctca ttcatcagtc
      421 cttcaactgg cagcacgggg tttttctcgg ttcggtggtc tcttccgaac gcaccgcggc
      481 cgccgacggc acagtcggtg cgatccgccg cgatcccttc gccatgctcc ccttctgcgg
      541 ctacaacatg gccgactatt tcgcccactg gctttgcctg ggcaaccgcc acgatgcgga
      601 caagctgccc aggatctttt atgtcaactg gttccgcaag aacgccgccg gccgtttcct
      661 ctggcccggc tacggcgaga acagccgcgt gctcaaatgg gtctttgaac gctgcagcgg
      721 cgccggtgag gccctcacca cacctatcgg ccatgttccg gccccgggcg ccctcgacgt
      781 gcgcgggctc gaaatcggcg aggccgagct ggcggagctc ttcagggtgg atcgcgaggg
      841 ctggatcagg gaagcggctt ccatccgcga gtattatgcg acctttgggc cgcgcttccc
      901 gcaacagctt tatgaggaac tcggcgccct tgaggggcgg ctgcgttagg tggctctgcc
      961 gttcccgccc cgggatccgg ggcgggaacg ggcggaagtc cttgacattt atccccggct
     1021 ttgttagatt gaaatgcaga gatgtcccgg ccgccgccaa ggatcggccg gagggagagg
     1081 aactaaaccg gacagcccga gtcggcttca cgggtcgttc cagagggagc aggatatgcg
     1141 agaaatagca gccccaatca tcagcgagca ggtcgccagg ctgtgcattg acgccaatta
     1201 ctatcttggc gctgatgtcc tctcagccct cgaggaggca ttggccaaag aggagtcgcc
     1261 caccgggcgc gccatcctcg aacaaatcat cgagaacgcc ggtatcgcca gggaagggga
     1321 gttccccatg tgccaggata ccggctttgc ggtttttttc gtcgaactcg gccaggaggt
     1381 agtggtcacc ggccaaaccc tcgaagaggc catcaccgag ggggtgcgca aggggtatgg
     1441 cgagggttat ttgcgcaagt cgattgtttc cgaccccctg cagcgggtca acaccaagga
     1501 caacaccccg ccggtgatct ggatcgagac caaacccggc cgggaactca agatcaccat
     1561 cgctcccaag gggggcggtt cagaaaacat gagtgaggtg gccatgctca ctccctccga
     1621 cggcgccgag ggcatcaaga atttcgtcgt cgacaaggtg cgccgcgccg gcagcaatcc
     1681 ctgtcccccc atcgtcgtcg gcgtgggcat cggcggcacc ttcgagaaag tggcctggct
     1741 ggcgaaaaaa tccctgctcc gcccggtcgg cgaccgcaac cccgatccct tctatgccgc
     1801 gatggagctc gagctgctcg agcgggtcaa caagctcggc atcggcccgc aggggctggg
     1861 cgggcgcacc accgccctcg atgtgcacgt cgaggtcttt ccctgccata tcgcatccct
     1921 gccggcggcg gtcaatatcc agtgccacgc cgcgcggcac aaatcggtgg tcatttgatc
     1981 aggaactctt ttttcgataa aggagtgtgg ctatggcgga agcaatcaga ataacgacgc
     2041 ccctgaccga cgatgtggtc aagaagctcc gcgccgggga cagcgtgttc atcaccggca
     2101 tcatctacac cgggcgggat gccgcccata aacgcatggt cgatctgctc gacaagggtg
     2161 aacccctgcc ggtcgatatc gccggtcatc tgatctacta tgtcggcccc tcgccggcac
     2221 gtcccggcaa accgatcggc tcggcaggtc ccaccaccag cggccgcatg aacgcctatg
     2281 ccccgcggct gctggcgatc ggctccaagg ggatgatcgg caagggcgaa atga
//
LOCUS       DAOPWU010000474         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_48906_length_2323_cov_4.059524, whole genome shotgun sequence.
ACCESSION   DAOPWU010000474 DAOPWU010000000
VERSION     DAOPWU010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48906_length_2323_cov_4.059524"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKM23_15315"
     CDS             <1..225
                     /locus_tag="PKM23_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HNY92892.1"
                     /translation="QTEKIHKTLCVVMYGSEYWNEVLNFDKMVDWGVISPEDLQLFHK
                     VDDVESAFAILKSHFETNFLNRQNYTHGSV"
     gene            212..1090
                     /locus_tag="PKM23_15320"
     CDS             212..1090
                     /locus_tag="PKM23_15320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNY92893.1"
                     /translation="MVQSELYRRLLRPARFPVQRQRAAGRLARRICTASIALVLAAAA
                     ACSPPRPGFLLDHDAVIRGDTSRRDLALVFTGGDFADGGPHIARVLADKGVKAGFFFT
                     GDFYRDPAKAELVHTLAGGDHYLGPHSSRHLLYAPWDKRDSSLVTREEFAADLRENLA
                     AMESFGLPDYGLDCFIPPYEWYNTRHVAWAAEMGLTLFNFTPGTSVNADYTTPEMVGY
                     RSSMAIWEKIIAREREDPHRLNGFILLIHIGTDPRRSDKFYLHLGALIDLLRAKGYTL
                     VRIDQLLERANRERSQ"
     gene            1087..2238
                     /gene="nagA"
                     /locus_tag="PKM23_15325"
     CDS             1087..2238
                     /gene="nagA"
                     /locus_tag="PKM23_15325"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNY92894.1"
                     /translation="MSGRYDMILKEVRLLERSAATDIAIRGGRIAAIGRLPGDAKRIL
                     DAGGLRIVPGFIDLHIQGAGGADVLDGTPEALQTMSQSLARLGTTGFLATSVAFAEGD
                     NRHLALAAESAGRYLGGAKLLGIHLEGPYINPEKKGGLNPRCIFAPEVRSLDAILAAC
                     GGALKMMTLAPEMPGGIELVRELLANGVVPALGHTGATYEQARAGFDAGIPHVTHVFN
                     AMPALNHREPGPLLALFEHPQATAQIISDGVHVHSGMVRLLSRLIGVERCVPITDGIQ
                     ATGLPEGRYLYNGREYESRDGAARYLDGTLIGSALGLAEVAWRFQRFTGCTSAQAAAS
                     ASYQAACVIGLGNRKGRIATDYDADLVILNQDNSVALTMIGGEVVWQNG"
BASE COUNT          408 a          765 c          717 g          433 t
ORIGIN      
        1 cagaccgaaa agattcacaa gaccctctgc gtggtcatgt acggcagcga atactggaac
       61 gaggtgctta attttgacaa gatggtcgac tggggggtga tcagtccgga ggacctgcag
      121 ctttttcaca aggtggacga tgtggagagc gccttcgcca tcctcaagag ccattttgaa
      181 accaattttc tcaaccggca gaattacact catggttcag tctgagctgt atcgccggct
      241 cctgcggccg gcgagattcc ctgtccagcg gcaacgggcg gctggacgcc tggcgaggcg
      301 gatctgtacg gcgtccatcg ccctggtttt ggctgcggct gcagcctgct ctccgccgcg
      361 gccgggattt ctcctcgacc atgacgctgt catccgcggc gacaccagcc gccgtgatct
      421 ggccctggtc ttcaccggcg gggatttcgc cgacggcggc cctcacatcg cccgcgtcct
      481 ggcggacaag ggggtcaagg cgggcttttt cttcaccggg gatttctacc gggatccggc
      541 caaggcggag ctcgtccaca ccctggccgg cggcgaccac tatctcggcc cccattcgag
      601 ccgtcacctc ctttacgccc cctgggataa gcgcgactcc tccctggtca cccgggagga
      661 gttcgctgcg gatctgcgcg aaaatctggc agccatggag tccttcggac tgcctgacta
      721 tgggcttgac tgcttcatcc ccccctatga atggtacaac acccggcatg tcgcctgggc
      781 cgccgagatg ggtctcaccc tcttcaattt cacccccggc acctccgtca acgccgatta
      841 caccaccccg gagatggtgg gctatcgctc ctccatggcg atctgggaaa agatcatcgc
      901 ccgggagcgg gaggatccgc accgcctcaa cggctttatc ctgctgatcc atatcggcac
      961 cgatccccgg cggagcgaca aattttacct tcatctgggg gccctgatcg atctcctgcg
     1021 cgcgaaaggg tacaccctgg tcaggatcga tcaactgctg gaacgtgcaa accgcgagag
     1081 gagccaatga gcggccgata tgatatgatt ctcaaagagg tgcgcctgct ggaacgcagt
     1141 gcggccaccg acatcgccat ccgtggcggg cgtatcgccg cgatcggccg cctgccggga
     1201 gatgccaagc ggattctgga cgccggcggg ctgcgcatcg tgccgggatt catcgatctg
     1261 cacattcagg gtgcgggcgg ggccgatgtg cttgacggca cccccgaggc gctgcaaacg
     1321 atgtcgcaga gcctggcgcg actcggcacg accggttttc tcgccaccag cgtggccttt
     1381 gccgaaggcg ataaccgcca cctcgccctg gcggccgagt cggcgggcag atatctcggc
     1441 ggggcaaagc tgctcggcat ccatctcgag ggcccctaca tcaaccccga gaaaaagggg
     1501 gggctcaatc cccgctgcat ttttgcgccc gaggtgcgga gcctggatgc gatcctggcc
     1561 gcctgtggcg gcgccctgaa gatgatgacc ctcgccccgg agatgcccgg gggcatagag
     1621 ctggtccgcg aactgcttgc caacggcgtg gtgccggcgc tcggccatac cggggccacc
     1681 tatgagcagg cgcgtgcggg ctttgacgcc ggcatccccc atgtcaccca tgttttcaac
     1741 gccatgcccg cgctcaatca ccgcgagccc ggtcccctgc tcgccctctt tgaacacccg
     1801 caggccaccg cccagatcat cagcgacgga gtccacgtcc attccggcat ggttcgactg
     1861 ctcagccggc tcatcggtgt ggaacgctgt gtgcccatca ctgacggcat ccaggcgacc
     1921 ggacttccgg aggggcgcta tctctacaac ggccgcgagt atgaatcccg cgatggggcg
     1981 gcccgctatc tcgacggcac cctcatcggc tcggcgctcg gtttggccga ggtggcctgg
     2041 cgcttccagc gtttcaccgg ctgcacatcg gcccaggccg ccgcctcggc gagttaccag
     2101 gccgcctgcg tgatcggcct cggcaaccgc aagggccgga tcgcaacgga ttacgacgcc
     2161 gatctggtca ttctcaacca ggacaacagc gtcgccctga ccatgatcgg gggcgaggtg
     2221 gtctggcaaa atggctgagg ggatgctggt ggggtgatgt tttgaagagc gacagctcca
     2281 acacgaagca tcttgcgggg gaattacgtc agcatcggca ctt
//
LOCUS       DAOPWU010000475         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_48959_length_2321_cov_5.075463, whole genome shotgun sequence.
ACCESSION   DAOPWU010000475 DAOPWU010000000
VERSION     DAOPWU010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48959_length_2321_cov_5.075463"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PKM23_15330"
     CDS             complement(<1..730)
                     /locus_tag="PKM23_15330"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNY92895.1"
                     /translation="MTICRKKFHIFALAICLMTAPMGVRASSNHEGGTSGAASDSIRY
                     HLDPVIVTASKISESQREIAASVAVLEEASLATAPSATLLGAIQQRVPGLYITERAVM
                     GYGVSGGAGTFSIRGVGGSPVTGVLVLRDGRPDMMGLFGHPLPDSYGALGVERVEVVR
                     GPASFLYGTNAMGGVINLISKRRHEEGFETRAKTALGAYNSRSFSLAHGGRSGRWDYY
                     LTAASDQTDGHRENSEYTGLHYTLH"
     gene            complement(809..2044)
                     /locus_tag="PKM23_15335"
     CDS             complement(809..2044)
                     /locus_tag="PKM23_15335"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNY92896.1"
                     /translation="MKKILVIAALTLIHCGYGGEEGRILSGLDLLVRDRFEPLAGKRV
                     GLITNHTAVTRDGRHAADLFAQAGNLQLVALFGPEHGVRGSLEAGALLDHEQDPRTGI
                     QVHSLYGEHNKPTPEMLADVEVLVFDIQDVGARFYTYISTLFYGLEAAAEKGIPFVVL
                     DRPNPVGGEIVEGPLLDLAHRSFVGIHQLPIRHGLTVGEVATLFNGEGWLAGGVKADL
                     RVIRMENWRRGLFYAETGLPWIKPSPNMTDPHTALLYPGTCLVEATNLSEGRGTSAPF
                     RQIGAPWHNAPALCRQLRAAGLAGVTIDTVTFTPVSLPGTATNNKHENRPCRGVTFTV
                     TDPGRFRAVTFGLHLLAAVRDLHADSLVIREAGMNRLDGDSRLTRALIEGAKVEKLLA
                     IARAEEARFIERRRPYLLY"
     gene            complement(2082..>2321)
                     /locus_tag="PKM23_15340"
     CDS             complement(2082..>2321)
                     /locus_tag="PKM23_15340"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNY92897.1"
                     /translation="GFHRALQEDFFSNVRGGSTSTYTVKQGDAIWAICNGLFDAPFWL
                     LQRYNRGENLLALKPGQTIVYPLLSPLSEGAVVNP"
BASE COUNT          408 a          688 c          821 g          404 t
ORIGIN      
        1 cgtgaagggt atagtggagg cctgtgtact ccgaattctc gcggtggccg tcggtttgat
       61 ccgaggcggc ggtgagataa tagtcccagc gcccgctccg gccgccgtgg gccagggaga
      121 aggagcggct gttgtaggcg ccgagggcgg ttttggcccg ggtttcaaaa ccctcttcat
      181 gccgccgttt gctgataaga ttgataaccc cgcccatggc gttggtgccg tagaggaaag
      241 aggcgggtcc gcggaccacc tccacccttt cgacgccgag ggcgccgtac gagtcgggca
      301 ggggatgtcc gaagagaccc atcatatcgg ggcggccgtc gcgcagcacc aggaccccgg
      361 tcaccgggct gccacccacg ccgcggatgg agaaggtgcc cgcccctccg gagacgccat
      421 agcccatgac cgcgcgctcg gtgatataga ggccgggcac ccgctgctgg atcgcaccga
      481 gcagggtggc cgaaggcgcc gtggcgaggc tggcctcctc cagcacggcg acgctggcgg
      541 cgatttcccg ctgcgactcg ctgatcttgg aggcggtcac aatgacggga tcgagatggt
      601 agcggatgga gtcggaggcg gctccgcttg tccccccctc atgattgctg gatgcccgga
      661 ctcccatggg agcggtcatc aggcagatgg ccagggcgaa gatatgaaat ttcttcctgc
      721 agattgtcac agtattccca tagtatgcat aatcattatt tcagagtaac agcggggccc
      781 gggatgctat tccccgcccg gggcggcctc agtagagcag gtagggccgg cggcgttcga
      841 tgaagcgggc ttcctccgca cgggcgatgg cgaggagttt ttccaccttc gcaccctcga
      901 tcagggccct ggtcagccgg ctgtcgccgt ccaggcggtt catgcccgcc tcgcggatga
      961 cgagagaatc ggcatgcaga tcgcgcacgg ccgcgagcag atgcagtccg aacgtgacgg
     1021 cgcgaaagcg cccgggatcg gtcactgtga aggtcacacc gcggcagggg cggttctcat
     1081 gtttgttgtt ggtcgccgtc cccggcaggc tgaccggggt gaaggtgacg gtatcgattg
     1141 tcactccggc gagtccagcc gcacgcagct gccggcagag ggccggtgcg ttgtgccagg
     1201 gcgcgccgat ctgacggaag ggagcggatg tgccccggcc ttcggagaga ttggtcgcct
     1261 cgaccaggca ggtgccggga tagagcaggg cggtgtgcgg gtcggtcata ttgggagaag
     1321 gtttgatcca gggcaggccg gtctccgcat aaaagagccc gcggcgccag ttctccatgc
     1381 ggatcacgcg cagatctgcc ttgactcccc ccgccagcca tccctccccg ttgaagaggg
     1441 tggccacttc tcccacagtg aggccgtggc ggatcggcag ctgatggatg ccgacgaaag
     1501 agcggtgggc gaggtcgagg agggggccct caacgatctc cccgccaacc ggattggggc
     1561 ggtcgaggac cacaaagggg atgcccttct cggccgccgc ctcgaggccg taaaagaggg
     1621 tgctgatgta ggtataaaaa cgggcgccga catcctggat gtcgaagacc agcacctcca
     1681 catccgcgag catctccggg gtgggcttgt tgtgttcgcc gtagaggctg tggacctgga
     1741 tcccggtgcg gggatcctgt tcgtgatcga gcagggcgcc cgcctccagg ctgccgcgca
     1801 ctccgtgctc cgggccgaag agggcgacca gctgcagatt gccggcttgc gcgaacaggt
     1861 cggcggcatg gcggccgtcg cgggtgacag cggtgtggtt ggtgatcagt ccgacccgct
     1921 tgccggcgag gggttcgaag cggtcgcgga cgagaaggtc cagaccggaa aggatcctgc
     1981 cctcctcccc gccgtatccg cagtggatca gggtcagtgc agcgatgacc aggatttttt
     2041 tcatcattta tcctttccgg aagaggggct ggccgtatgg ctcagggatt cacgactgcc
     2101 ccctcggaga gcggcgagag caggggatag acgatagtct gccccggctt cagggcgagc
     2161 agattctccc cccggttgta gcgctgcagg agccagaagg gagcatcgaa gaggccgttg
     2221 cagatggccc agatcgcatc gccctgcttg acggtatagg tggaggtgct gccgccgcgg
     2281 acattggaaa agaaatcctc ctggagggcg cggtggaacc c
//
LOCUS       DAOPWU010000476         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_48991_length_2320_cov_4.667108, whole genome shotgun sequence.
ACCESSION   DAOPWU010000476 DAOPWU010000000
VERSION     DAOPWU010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48991_length_2320_cov_4.667108"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKM23_15345"
     CDS             <1..463
                     /locus_tag="PKM23_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNY92898.1"
                     /translation="NAAYYGDHVELTDGGDDFASQVGVGAVIGTKFTWPEGAARRPRN
                     DLTQAREKEWKKWTDIYLEHMLSKGIYRGELYDLGFDRPETHAIAKESRMYYAFYADQ
                     YSGEVELRGLENKSYQIKDYVVGHSFGSVRGPKAKIKVDFHNHLLLMATPE"
     gene            481..>2320
                     /locus_tag="PKM23_15350"
     CDS             481..>2320
                     /locus_tag="PKM23_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92899.1"
                     /translation="MHMKNWIICLTGALLLLGCASESPGPEGAAKALNWTHFVRIGGH
                     GLNMERISEIIKSVQETGVFGIETDNDITGRYDSFLDPAEKLAAIKKMADEAHRIGNY
                     AFVYIAGTECITANADQTPHSFFKDHPDWVQRKITGEPAVFGGGSAFWISEGDEDVWI
                     SPYAMEWRKIYMERVRQIAAAGIDGVYVDIPYWMTHFDGWEESWASFDDYTVAAFKQQ
                     TGLDAKTQIRLGDFTDPGFLKWVDFRIQTLTDFMVEINDNVKSVNPACMTIPEIYPGI
                     GEEAVRVGSDVYEMYVAMDAIAHEYSAGGYTAAERDPISWLTYMEGMFTFRAFAEGKA
                     SWMLSYSWDDNAGIHPGDAMKNLALSQVMAGTNFWDARGHVMSGSNDFATRTEIFKWI
                     GANEKTLYHPREPIQPVGLYFSPLTRNYFADDFIASYHGLFHLLLNAHVEFEVVTPRT
                     LAGFSGGVLILPDVRILSAEELAQLEKITARGGKILLTGESGAYGRERQRLEVNPLHQ
                     LLGINGAAATGAINLPECPGKGGLALIRKEGFASISTTGAPLQRLLVDFQQQLSALGY
                     APAIRLEASPMVVAQIARVDGRPHLFMANFNGLEGGRNANQTPVRAA"
BASE COUNT          486 a          708 c          676 g          450 t
ORIGIN      
        1 aaatgcggct tattatggcg accatgtcga attgaccgat ggcggggatg atttcgcctc
       61 ccaggtcggt gtcggcgccg tgatcggcac caagttcacc tggccggaag gagcagcccg
      121 ccggccgaga aacgacctca cccaggcgcg ggaaaaggaa tggaagaaat ggaccgatat
      181 ctacctggag cacatgctca gcaagggcat ctaccgcggc gagctttatg acctgggctt
      241 tgaccgtccc gagacccacg caatcgccaa agagagcagg atgtactatg ccttttacgc
      301 cgaccagtac tcgggcgagg ttgagctgcg cggactggag aataaatcct atcagatcaa
      361 ggattatgtc gtcgggcact ccttcggcag cgttcgggga ccgaaagcca aaatcaaggt
      421 tgacttccat aatcatctcc tgctcatggc gactcctgag tagaaccctg aggaaaaggt
      481 atgcacatga agaactggat catttgcctg accggcgcgc tcctgctgtt gggttgtgcg
      541 tcggaatccc ccggacctga aggggctgcc aaggccctga actggaccca ctttgtccgc
      601 atcggcggcc acggcctcaa catggagcgg atatccgaaa tcatcaagag cgttcaggag
      661 acgggggtct tcggcataga aaccgacaac gacatcactg gccgctatga cagttttctc
      721 gatccagccg aaaagctggc ggcgatcaag aaaatggccg acgaagcaca tcgcatcggc
      781 aattacgctt ttgtctacat cgccggtacc gagtgcatca ccgcaaatgc ggaccagaca
      841 ccacactctt tcttcaagga tcaccccgac tgggtgcagc gcaagatcac cggcgagccg
      901 gcggtctttg gcggcggctc cgctttctgg atcagcgaag gggatgagga tgtctggatc
      961 agcccctatg caatggagtg gcgcaagatc tatatggagc gggtccgcca gatcgcagcc
     1021 gccggcatcg acggggtcta tgtcgatatc ccctactgga tgacccactt tgacggctgg
     1081 gaggagagct gggcgagctt tgacgattat acggtcgcgg ccttcaaaca gcagaccggc
     1141 ctggatgcca aaacccagat ccggctcggg gattttactg atccgggctt tctcaaatgg
     1201 gtcgatttcc gcattcagac cctcaccgat ttcatggtcg agatcaacga caatgtcaag
     1261 tcggtcaatc cggcctgcat gaccattccg gagatctatc ccggcatcgg cgaagaggcc
     1321 gtgcgcgtcg gcagcgacgt ctacgagatg tatgtcgcga tggatgccat cgcccatgaa
     1381 tacagcgccg gcggctacac cgccgccgag cgcgatccca tctcctggct gacctacatg
     1441 gaggggatgt tcaccttccg ggccttcgct gaaggcaagg cctcgtggat gctcagctat
     1501 tcctgggacg ataatgcagg catccatccg ggcgatgcga tgaagaacct ggccctctcc
     1561 caggtaatgg cggggaccaa tttctgggac gcccggggcc atgtcatgtc cggttccaat
     1621 gactttgcca cgcgcacgga gatctttaaa tggatcgggg ccaacgaaaa gaccctctat
     1681 catccgcgcg agcctatcca gccggtcggc ctctatttct ctccgctgac ccgcaactac
     1741 ttcgccgatg atttcatcgc ctcctaccac ggtcttttcc atctgctgct caatgctcat
     1801 gttgaatttg aggttgtaac cccgcgtacc ctggccggat tcagcggcgg ggttttgatc
     1861 ctgccggatg tccgcatcct cagtgcagag gagctggcgc agcttgaaaa gataaccgcc
     1921 cggggaggga aaatactgct caccggggag agcggcgcct atggccggga acggcagcgg
     1981 ctggaggtca atcccctgca ccagctgctc gggatcaacg gcgccgccgc caccggcgcc
     2041 atcaacctgc cggagtgccc aggcaagggc ggtctcgctc tgatccggaa ggagggattc
     2101 gcctccatct cgaccaccgg agcccccctc cagcgcctcc tcgtcgattt ccagcagcag
     2161 ctgagcgccc tcggctatgc gccggccatc cgcctcgagg cctcgcccat ggttgtggcc
     2221 cagatcgccc gggtggacgg caggccgcac ctttttatgg ccaatttcaa cggtctggag
     2281 ggcggccgca atgccaacca gacccctgtc cgcgctgccc
//
LOCUS       DAOPWU010000477         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_49203_length_2313_cov_4.430027, whole genome shotgun sequence.
ACCESSION   DAOPWU010000477 DAOPWU010000000
VERSION     DAOPWU010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49203_length_2313_cov_4.430027"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKM23_15355"
     CDS             <1..612
                     /locus_tag="PKM23_15355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNY92900.1"
                     /translation="RIAREIHDSLGQQLTALQLEVTAATHALKGGVEVPKALQDAVHT
                     IEESITMAQNLCYDLRPPLLDDFGLEAALRDHVTEYQEKWGIAVDFTAEKLDHLLSRD
                     AETALFRVAQEAMNNVLKHAQARMVRVRLGLYDGRIFLRIQDDGCGFEPEPVQSRGRS
                     DHFGLMTMNERIELLGGELMVESRPGSGATITAWLPMQRREER"
     gene            609..1265
                     /locus_tag="PKM23_15360"
     CDS             609..1265
                     /locus_tag="PKM23_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNY92901.1"
                     /translation="MKIHSIMIADDHAVLREGLRLLLSSYPQFEVIGEADDGMTAVAT
                     ALQIKPDVLLLDIAMPNMRGIEAILELKRLEPEIKILVLSMHDREEYVLQAMKNGADG
                     YILKKSAAAELVAALNHVISGEIYLSPSVSRHLVRNWLRDEESMAGVSRAETELSERE
                     RTVLKLIAEGRSNKEVASLLHISAKTVETHRARIMSKLELRTVPDLVRYAIKSGLVDL
                     "
     gene            complement(1511..1669)
                     /locus_tag="PKM23_15365"
     CDS             complement(1511..1669)
                     /locus_tag="PKM23_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNY92902.1"
                     /translation="MERYICTNCGFVYDPIEGDPERGIPPGTPFEDLPEEWVCPVCYV
                     TQDRFDPL"
     gene            complement(1698..>2313)
                     /locus_tag="PKM23_15370"
     CDS             complement(1698..>2313)
                     /locus_tag="PKM23_15370"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNY92903.1"
                     /translation="LKERCIAEKLLEPKVVYGYYPCSSCGEEVILYHPESSQEWLRFA
                     FPRESRPPHRCIADYFQPEGSGIRDLIALQVVTVGARASEEAKRLYESNAYKEYLLFH
                     GLSVESAEALAEYWHQLIRRELGIADEDGASIEDFVVQKYRGSRYSFGYPACPDLSEN
                     RKLFTILRPERIGVTITDGELMTPEQSTSAFIVHHPQAKYFNVA"
BASE COUNT          461 a          664 c          716 g          472 t
ORIGIN      
        1 cggatcgcgc gagagatcca tgattcactc ggtcagcagc tgacggcgct gcagctggag
       61 gtgacggcgg ccacccatgc cctgaagggg ggtgtggagg tccccaaagc cttgcaggat
      121 gcggtccaca ccatcgaaga gtcgatcacc atggcgcaga acctctgcta tgatctgcgg
      181 ccgcccctgc tggacgattt cggactcgag gccgccctgc gcgatcatgt caccgagtat
      241 caggaaaaat gggggattgc ggtcgatttc accgccgaga agctcgacca cctcctcagc
      301 cgggacgcgg agacggccct tttccgggtg gcgcaggagg cgatgaacaa tgtgctcaaa
      361 cacgcccagg cccgcatggt ccgggtccgg ctgggactgt atgacgggag gatttttctg
      421 cgcatccagg atgacggttg cggctttgag ccggaacctg tacagagccg tggacgcagt
      481 gatcactttg gtctgatgac gatgaacgaa cgcatcgagc tgcttggggg ggagctgatg
      541 gtggagagcc ggcccgggag cggcgccacc atcaccgcct ggctgccgat gcagagaagg
      601 gaggagcgtt gaaaattcac agcatcatga tcgccgacga ccacgctgta ctgcgtgagg
      661 ggctccggct gctgctctcc tcctatccgc agttcgaggt catcggcgag gccgacgacg
      721 gcatgaccgc ggtcgctacc gccctgcaga tcaagccgga tgtgctgctg ctcgacatcg
      781 cgatgccgaa catgcgcggc atcgaggcga tcctcgagct caagcgcctc gagcctgaaa
      841 taaagatcct ggtgctgagc atgcacgacc gtgaggagta tgtcctgcag gcgatgaaaa
      901 acggggcgga tggctatatc ctgaaaaagt cggccgcggc ggagctggtt gccgccctga
      961 accatgtcat ctccggcgaa atctacctct caccctcggt ctcgcgccat ctggtacgga
     1021 actggctgcg cgacgaggag agcatggccg gggtgagccg cgctgaaacc gagctttcgg
     1081 aacgcgaacg gactgtactc aagctcattg ccgagggccg ctcgaacaag gaggtggcga
     1141 gtctgctcca catcagcgcc aagacggtgg agacccatcg cgcccgcatc atgagcaagc
     1201 tcgagctgcg tacggtgccg gacctggtgc gctatgccat taaaagcggc ctggtcgacc
     1261 tttaaaatgc ttgcgttttt tttaaaattt tagtaaaatg gagtgcggtt gagttttgcc
     1321 cgccatcagg gtgtagggaa tttcctgact gacgaataca gagtattccc tattgttttc
     1381 cagatgagaa ttttgtatct taatagcgtc aagagacaat gacattgccg ggcgtcccct
     1441 cggttactgg taacccccct aattccagtg ccatggacgg ctcggcattt ttttttgccc
     1501 ttttttcaaa tcaaagggga tcaaaccggt cctgggtgac gtagcagacc gggcagaccc
     1561 actcctcagg cagatcctcg aaaggggtgc cgggggggat gccccgctcc ggatcgccct
     1621 cgatcgggtc atatacaaag ccgcagttgg tgcagatgta gcgttccatc ttcctctctc
     1681 cgtgttttct agcttcctca agcgacgttg aaatatttgg cctgcgggtg gtgcactatg
     1741 aaagccgagg tgctttgctc cggcgtcatc aactccccat cggtgatggt caccccgatg
     1801 cgctcgggcc gcaggatggt gaagagcttg cggttctcgc tcaggtcggg gcaggcggga
     1861 tagccgaagg agtagcgcga gccccggtat ttttgcacga caaaatcctc gatgctggcg
     1921 ccgtcctcat cggcaatgcc cagttcccgg cggatgagct gatgccagta ttcggccagg
     1981 gcctcggcgc tctcgacgct caatccgtga aagaggagat actccttgta ggcattgctc
     2041 tcatagaggc gtttggcctc ctcgctcgcc cgcgccccga ccgtgaccac ctgcagggcg
     2101 atgaggtcgc ggatcccgct gccctcgggc tggaaatagt cagcgatgca gcgatgggga
     2161 ggtctgcttt cgcgcggaaa ggcgaaacgc agccactcct gactgctctc cggatggtag
     2221 aggatcacct cctccccgca gctgctgcag ggatagtagc catagaccac tttgggctcg
     2281 agcagttttt ctgcgataca gcgctccttg aga
//
LOCUS       DAOPWU010000478         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_49397_length_2306_cov_3.612172, whole genome shotgun sequence.
ACCESSION   DAOPWU010000478 DAOPWU010000000
VERSION     DAOPWU010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49397_length_2306_cov_3.612172"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..322
                     /locus_tag="PKM23_15375"
                     /pseudo
     CDS             38..322
                     /locus_tag="PKM23_15375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
     gene            455..826
                     /locus_tag="PKM23_15380"
     CDS             455..826
                     /locus_tag="PKM23_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNY92904.1"
                     /translation="MIAKHHVYRCGVCGNMVEVVAVGGGTLVCCGQPMNLQEENTSDG
                     ATEKHVPVVEKNGTVKVTVGSVLHPMTEAHYIQWIEVVTASKVLRKYLKPGEEPVAVF
                     TVDEPVLKVREYCNLHGLWAV"
     gene            871..2217
                     /locus_tag="PKM23_15385"
     CDS             871..2217
                     /locus_tag="PKM23_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNY92905.1"
                     /translation="MKQLSVYLVFMLLLCGIASAQVAPSGDALLGSKKYEQYEKPDVC
                     KSCHPDFYQQWRQSMMSQSYIHYWDEIEYFDLAVPHAEKVPGLKGPVDGCNGCHTPLA
                     FMAGDVTPPRPSANSRANEGVSCEVCHTISSWSGDVPHNFSYISTPGRKKFGAKPGLV
                     SPHHDTEQNALYEQTEFCGNCHNEKNPFGIWVKSTQIEWKEGPYAAQGVPCQQCHMPK
                     AKGKNATMAKEDMVAQHLFHGAHDGGKVAGTIELRIHPSELEVPYDGTVVLSAQLFNA
                     KTGHKFPSGSVEDRIVWLHVTATDAAGKIYHLPVDKKGFAGEEYTIASDVLAYQDMGV
                     PKGIANFAGVQRDGVPVGDRIFRMPYLDPEGRMTIMQWNTKSLGVDYRIGPRETKTET
                     YTWKLPDDVAAGPVTIIAQLNYQKLVKPVADFLGVPADESEIVPVNIAKTTFEIYE"
BASE COUNT          520 a          640 c          673 g          473 t
ORIGIN      
        1 cggcttcgcc ctgtttatca tctccaatca gtccggtgtg ggtcggggtt tgatcacgcc
       61 ggaggatgtg gaggcggtga atcgcgaaat ggtgcggcag ctgggagcgg atttttttga
      121 tggtatttat ctttgctttg ctgctcctga ccagcccgac aataactgcc ggaagcctaa
      181 tcccggcatg gtctggcagg cgcgggatga ccatgacctt gacctggaac gctccttttt
      241 catcggcgac aagctcatcg acgtccagtg cggccggaat gccggctgcc attcggttct
      301 tttgtataat tcccaggatg atcctgaagg cagggcgtct gcgttggccg ctgccgactt
      361 taccgcctcc acccttccgg aggcggctcg ctggatcatc gcaacccatc aagagcgggc
      421 gacacactcg caccacaaag agaggaaaaa cgaaatgatc gccaaacatc acgtttatcg
      481 ctgcggggtc tgcggcaaca tggtcgaagt ggtcgctgtc ggcggcggca ccctggtctg
      541 ctgcggccag ccgatgaatc ttcaggagga gaacaccagc gacggcgcca ccgaaaagca
      601 tgtgccggtg gtggagaaaa acggcacggt gaaggtcacg gttggcagtg ttctccaccc
      661 catgactgag gcgcactaca ttcagtggat tgaagtggta acggcgagca aggtgctgcg
      721 taaatacctc aagccgggcg aggaacccgt cgcggtgttc accgtggatg agccggtgct
      781 taaggtgcgc gaatactgca atctgcacgg gctgtgggcg gtctgaaatt caaggtacaa
      841 atcagttggg aacactgtca ggagggaacc atgaaacagc tctccgttta tctcgtgttt
      901 atgctgctcc tctgcggcat tgcatctgcc caggtcgcgc cgtcgggtga cgccctttta
      961 ggcagcaaaa agtacgagca gtacgaaaaa ccggatgtgt gtaaaagctg ccaccctgat
     1021 ttctatcaac agtggcggca gtcaatgatg tctcagtcct atatccacta ttgggatgag
     1081 atcgagtatt ttgatctcgc cgtgccccac gccgaaaaag tgcccggact caaggggccg
     1141 gtggatggct gcaacggctg ccatacgcct ttggccttca tggccgggga tgtgaccccg
     1201 ccgcggccat cagcaaactc acgcgccaac gaaggggtca gttgtgaggt ttgccacacc
     1261 atcagcagct ggtccggcga tgtgccgcac aatttcagct atatctctac gcccgggcgt
     1321 aaaaaattcg gcgccaaacc tggactggtt tcaccgcacc atgacactga acagaatgct
     1381 ctctatgagc aaaccgaatt ctgcggcaac tgccataacg aaaagaatcc attcggcata
     1441 tgggtgaaat cgacccagat cgagtggaaa gaagggccct atgccgctca gggggtgccc
     1501 tgccagcagt gtcacatgcc caaggcaaag ggcaagaatg ccaccatggc caaagaggat
     1561 atggtggcgc agcatctttt ccatggcgcg catgatgggg gcaaagtcgc cggcactatc
     1621 gaactgcgca ttcacccttc cgagctggag gtgccttatg acggcaccgt ggtgctgagc
     1681 gcccagctct tcaacgccaa gaccgggcat aaattcccct ccggctcggt ggaggaccgc
     1741 atcgtctggc tgcatgtcac agcgaccgat gccgccggca agatttacca tctgccggtc
     1801 gacaaaaagg gttttgcagg cgaagaatat accattgcca gtgatgtgct cgcctatcag
     1861 gacatggggg tgcccaaggg catcgccaat ttcgccggcg tgcagcgcga cggagtcccc
     1921 gtgggcgacc ggatcttccg catgccctac ctggatcccg aggggcggat gacgatcatg
     1981 cagtggaaca ccaagtccct gggcgtcgat taccgcattg gcccgcgtga aaccaagacc
     2041 gaaacctata cctggaaatt gccggatgat gtggcggcgg gtcccgttac aattattgca
     2101 cagctcaatt atcaaaagct ggtcaagccc gtggccgatt ttctcggcgt gccggccgat
     2161 gaaagcgaga tcgttcccgt caacatcgcc aagaccacct ttgaaatcta cgaataagcc
     2221 caaggaggca agtcatgaaa cgagtaccag gaatcctgat cctctttgca gttctactgg
     2281 gggtgcagtg gttgatcgta ccggat
//
LOCUS       DAOPWU010000479         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_49643_length_2298_cov_3.575568, whole genome shotgun sequence.
ACCESSION   DAOPWU010000479 DAOPWU010000000
VERSION     DAOPWU010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49643_length_2298_cov_3.575568"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..755
                     /locus_tag="PKM23_15390"
     CDS             231..755
                     /locus_tag="PKM23_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HNY92906.1"
                     /translation="MITLISTWAIPFLLFVIPLYAFIKKIKVYEVFIDGAKEGFNVAV
                     RIIPFLVAILVAIGMFRASGAMDVFVYLLTPVTNLIGMPAEVLPAALMRPLSGSGSLG
                     IITELLKSHGSESLIGRMASTIYGCSETTFYVVAVYYGSVQIKNVRSSIWVGLTADLA
                     GILGTVFICRYLFM"
     gene            834..1724
                     /locus_tag="PKM23_15395"
     CDS             834..1724
                     /locus_tag="PKM23_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNY92907.1"
                     /translation="MKGGFLMPVQMHSMGWLPDYPDFRDYTTEHAAISPLLKKIGLTG
                     SHTKSLPESADLRAWCSTIEDQGSLGSCTAQAGASMVEYFAHRSMDRYTDVSRLFLYK
                     VTRNLMRQKGDTGAFIRTTMGALVLFGVPPELYWPYKEKDFDKEPPAFCYAFAQNYQA
                     ISYYRLDAAGLKKNEVLQAIKSNLAAGLPSMFGFSVFSSISQAGENGQIPYPMPGEKI
                     LGGHAVMAVGYDDKIEIANTNPDGPTCKGAFLIRNSWGPAWGLEGYGWLPYEYLLKGL
                     AIDWWSIIKNEWIETGDFEP"
BASE COUNT          465 a          711 c          577 g          545 t
ORIGIN      
        1 tcatcggctc gaccatcttc gcctccaccc tggcgaccat cttcggcgtc ctcgccgcca
       61 agagctttct cgccctcgac gcgggatggg gccactttgt taaaagcgta cgcgcagcct
      121 ggcgcttctt cctcatcacc gccctggcga tcgccgggct catcgtgctg gtcaagaccg
      181 gtattatcgc cgcccttttc tctttgctgc cgcccaatct cttccgcagc ctgatcaccc
      241 tgatctccac ctgggcaatc cctttcctcc tcttcgtcat ccctctctat gctttcatca
      301 agaaaataaa ggtttatgag gtttttatcg acggagccaa ggagggtttt aacgtcgccg
      361 tccgcatcat ccccttcctg gtggccatcc tggtggctat cggtatgttc cgcgcctccg
      421 gcgccatgga cgtgtttgtc tacctgctca cccccgtgac caaccttatc ggcatgcccg
      481 ccgaggtgct gccggcggcc ctgatgcgcc ccctctcggg cagcggctcc ctcggcatca
      541 tcactgaact gcttaaaagc cacggctcgg agtccctgat cggccgcatg gcctcgacca
      601 tctatggctg ctccgagacg accttttatg tcgtggcggt ctattacgga tcggtccaga
      661 tcaagaatgt gcgctcctcg atctgggtgg gactgaccgc cgatctggcc ggcatcctcg
      721 gcaccgtctt tatctgccgc tatctcttca tgtaaaaagt actttacatt ttccccgcca
      781 gaatgtaact tgcattgagg gagcggtgcg ccaaacgcgc tgtcttccct cccatgaaag
      841 gaggctttct tatgccagtg cagatgcatt ccatggggtg gctgccggat tatcccgatt
      901 tccgcgacta taccaccgag catgccgcca tcagtccgct tcttaaaaag atcggactga
      961 caggtagcca tacaaaaagc ctgcccgaga gcgcggacct gcgcgcctgg tgttccacca
     1021 tcgaggacca gggcagcctg ggctcctgca ccgcccaggc cggggccagc atggtggagt
     1081 atttcgccca tcgttcaatg gaccgttata ccgacgtctc caggcttttc ctttacaagg
     1141 tcacccgcaa tctcatgcgc cagaagggcg acaccggcgc cttcatccgc actaccatgg
     1201 gggctctggt cctcttcggc gtcccccccg aactttactg gccctacaag gaaaaggatt
     1261 tcgacaagga gccgccggcc ttttgctacg ccttcgccca aaattatcag gcgatcagct
     1321 attaccgtct ggatgcggcc ggcctcaaga agaacgaggt gttgcaggcg atcaagagca
     1381 atctggcggc cggcctgccc tcgatgttcg gtttttccgt cttcagctcg atctcccagg
     1441 ccggtgagaa cggccagatc ccctatccga tgccaggtga aaagatcctc ggcggccacg
     1501 cggtcatggc ggtcggctat gacgacaaga tcgagatcgc caataccaat cccgacggcc
     1561 cgacctgcaa gggggctttc ctgatccgca actcctgggg cccagcctgg ggcctggagg
     1621 gctacggctg gctgccctac gaatacctcc tcaagggtct ggccatcgac tggtggtcga
     1681 ttatcaagaa tgagtggatc gaaaccgggg attttgagcc gtaattacaa gtcctttccc
     1741 tgagatggcg tcacttcagg gaaaccctga tttggttgtg ctaatttttt atacatttta
     1801 cttgcataaa atagatgtaa ttcgttaatt ttcgtatcaa attactttca gagcccgttg
     1861 tctgcctcac tgtacccata cagccggaag attaccgcaa ggctctggag aaacagcttg
     1921 ggcggctgct gccatcgctc tattaattcc ccctgccgag ctctatgagc caattaaatt
     1981 ggttcatcct gcgaatgcag caaaggcgtg ccaaatcgat caggacgcat gactgcacac
     2041 gacaacgcaa tccattcacc catacgcgcc tcttttaatg ctgttcctta ttcccgggaa
     2101 ggaggtgaat agagctgtta atgatgcccc ggggctgcag gtgatctccc ttacagccca
     2161 acagcaggat ccgtcatgat gcaaaccatg atccatccag atcgcagtga agtcatttac
     2221 tatcaaggag aaaagtatgc gaagcaaaac aattgttact attctggcag tggtgctcgc
     2281 agcctcgctg acattcgc
//
LOCUS       DAOPWU010000480         2286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_50043_length_2286_cov_4.652622, whole genome shotgun sequence.
ACCESSION   DAOPWU010000480 DAOPWU010000000
VERSION     DAOPWU010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50043_length_2286_cov_4.652622"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1824
                     /locus_tag="PKM23_15400"
     CDS             271..1824
                     /locus_tag="PKM23_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNY92908.1"
                     /translation="MESATLPGRGKVAAPQLSILIEIHQALSRSLNMQESLTVTLQIL
                     HNSYQILSGAILLCDAAGPSLSLAAAIGLPPREGNAIYRFNEGLCGRIAETGKPVVVP
                     RVSKEPLFLNRLGSWESALRPEQSFIGVPIALDYRTLGVLFINLPYTPRRDYDNALNF
                     LTLVASALVQRLHIRQLMEAEQQKLLTENVILKQQLRQENRLQNIIGNSREMRELYEK
                     VAQVAHAPTTVLLRGESGTGKELVAQAIHYNSPRSEAPFIRVNCAAIPENLIESEFFG
                     YEKGAFTGAVARKKGRFELADGGTIFLDEIGDLSPMTQVKLLRVLQEHEFERVGGLET
                     IKVDVRIIAATHADIEELMARGAFREDLYYRLNVFTIFLPPLRERKGDILLLADHFML
                     KYGRQHGKSIQRISTPAIDMLMRYHWPGNVRELENCIERAVLVCEDQVIHSYHLPPTL
                     QTAESSGTLPRWSLPEAVASHEKELIQEALKSCRGIRARAARMLGITERIITYKINRY
                     KIDVDYYRG"
     gene            1930..>2286
                     /locus_tag="PKM23_15405"
     CDS             1930..>2286
                     /locus_tag="PKM23_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92909.1"
                     /translation="MKKLSILFLFLALQLSLQAGEVTRTGLQQVRPAIPISRGHLYAQ
                     IPMRFFFQDQILELAPDLLTGETYWDIQGGLALFYGLNDHVDFALHQIIYQDNHKPGT
                     GYNLPDDLFLRARVANF"
BASE COUNT          476 a          821 c          546 g          443 t
ORIGIN      
        1 caaaccattg tttattatgc tgcataatta aaaagccgcc gcgataaaaa aaagctggct
       61 atttagcgca ttatttctta catttatgta gctttccagg ttgaagcgcc atttttgtcg
      121 ttagtatgcc aaccctcacg gcctttccgg gcggtggtgc aaatccgtac cttacagccg
      181 cccccatcag gcccgaaaga tgagcctgta atctacaatt atgtagctat cattaaatat
      241 attacaaatt tgtagttttt tggagccctt atggagtccg caaccctccc cggccgcggc
      301 aaggtggccg caccccagct ctccatcctg atcgagatcc atcaggccct gagccgcagc
      361 ctcaacatgc aggaatccct caccgtcacc ctgcagatcc tccataactc ctaccagatc
      421 ctctccgggg ccattctcct ctgcgacgcc gccggcccct ccctcagcct tgccgccgcc
      481 atcggcctgc ccccccgcga aggcaacgcc atctaccgct tcaacgaggg gctctgcggc
      541 cgcatcgccg aaaccggcaa accggtcgtc gtccccaggg tgagcaagga acccctcttc
      601 ctcaaccgcc tcggcagctg ggaaagcgcc ctccgccccg aacagagctt tatcggcgtg
      661 cccatcgccc tcgactaccg caccctcggg gtcctcttca tcaatctgcc ctacaccccc
      721 aggcgcgact atgacaacgc cctcaacttt ctcaccctgg tcgcctcggc cctggtccag
      781 cgcctccaca tccgccagct catggaggcc gaacagcaga aactgctcac cgaaaatgtc
      841 atcctcaaac agcagctccg ccaggaaaac cgtttgcaga acatcatcgg caacagccgc
      901 gaaatgcgcg aactctacga aaaggtcgcc caggtcgccc atgcccccac caccgttctg
      961 ctgcgcggcg aatcgggaac cggcaaggag ctggtcgccc aggccatcca ctacaattcc
     1021 ccgcgcagcg aagccccctt catccgcgtc aactgcgccg ccatcccgga aaacctcatc
     1081 gagtccgagt tcttcggcta tgaaaagggg gccttcaccg gcgcggtcgc gcgtaaaaag
     1141 ggacgcttcg agctcgccga cgggggcacc atcttcctcg acgagatcgg cgacctctcg
     1201 cccatgaccc aggtcaaact cctgcgcgtc ctccaggagc acgaattcga gcgcgtcggc
     1261 ggcctcgaga ccatcaaggt ggatgtgcgc atcatcgccg ccacccatgc cgatatcgag
     1321 gagctgatgg cgcgcggggc cttccgcgag gatctctact accgcctcaa cgtcttcacc
     1381 atcttcctcc cccccctgcg cgagcgcaag ggcgacatac tccttctcgc cgaccacttt
     1441 atgctcaaat acggccgcca gcacggcaag tcgatccagc gcatctccac accggccatt
     1501 gatatgctca tgcgctacca ctggccaggc aatgtgcgcg aacttgaaaa ctgcatcgaa
     1561 cgggcggtgc tggtctgcga ggaccaggtg atccacagct accacctgcc gccgaccctg
     1621 cagaccgccg agagcagcgg caccctgccc cgctggtccc tgcccgaggc cgtcgcctcc
     1681 catgagaaag agctcatcca ggaggccctc aaatcctgcc gcggcatccg cgcccgcgcc
     1741 gcgcgcatgc tcggcatcac cgaacgcatc atcacctaca agatcaaccg ctacaagatc
     1801 gacgttgact attaccgggg ttgagcccgg gacgtgctct gcgtcaagga agcctcaaaa
     1861 agatattgct tccggcgctg cagtttgata cattacagct caatactcgt tgacctgtca
     1921 ggcatcccga tgaaaaagct ctctatcttg ttcctgtttc tcgctcttca gctctccctt
     1981 caagccgggg aggtcacccg caccggcctc cagcaggtgc ggccggcgat cccgatcagc
     2041 cgcggccatc tctacgccca gattccgatg cgcttctttt ttcaggacca gatcctcgaa
     2101 ctggcccccg atctgctcac cggagaaacc tactgggata tccagggggg actcgccctc
     2161 ttttacggtc tcaacgacca tgtcgatttt gctttgcacc agatcattta ccaggacaac
     2221 cataaaccgg gcacgggcta caatctcccc gatgacctct tcctgcgcgc ccgggtggcc
     2281 aatttc
//
LOCUS       DAOPWU010000481         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_50864_length_2263_cov_3.266757, whole genome shotgun sequence.
ACCESSION   DAOPWU010000481 DAOPWU010000000
VERSION     DAOPWU010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50864_length_2263_cov_3.266757"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1264)
                     /gene="gcvT"
                     /locus_tag="PKM23_15410"
     CDS             complement(182..1264)
                     /gene="gcvT"
                     /locus_tag="PKM23_15410"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNY92910.1"
                     /translation="METRKTALYDVHQQAGAKLVEFAGYYMPIQYRGIIEEHRRVRTT
                     VGLFDVSHMGEFIVKGSNAEAFLQKITINDVSKVDVRQVQYSAMCYPEGGIVDDLLIY
                     RFPDHYLMVVNAANLDKDWEWANRHLIGGVTLTNESDRYSLLALQGPMAQKTLQKLTA
                     LDLNKIDYYWLDEAEAAGVPMMISRTGYTGELGYELMMPNESAVKVWKAVMAAGAEFD
                     IEPIGLGARDTLRLEMKYCLYGNDIDQTTNPLEAGLGWITKLNKGEFISREALLQIKA
                     AGLKRKLIGFDMPGRAFPRHGYAIYSGGERVGVVTSGTFSPSLEKGIGTGYVPVELAA
                     VGTPLEIEIRNSRMPATVCETPFYKK"
     gene            complement(1409..>2263)
                     /gene="rseP"
                     /locus_tag="PKM23_15415"
     CDS             complement(1409..>2263)
                     /gene="rseP"
                     /locus_tag="PKM23_15415"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNY92911.1"
                     /translation="YVKLSGMIDESMDTGGVKGEPWEFMSKPVWVRMLVLFAGPLANV
                     LLTIFVFTVSVFITGIPEPVGPIVGRVMENMPAQTAGLQPGDRIVRIDGAPISSWDDL
                     IRIIHASPEKEIKIEWERSGAPMSALITPKLDKMLGYGLVGIDAKTINVRPGFFKGMA
                     VGWQSTWNLTRMMFRSFGMLFSGDVSVKEGLAGPVRIAQMTGETAKAGFGSLVMFMAL
                     LSLNLGIINLFPIPALDGGHLLLLAYEGVTRKPISTKVRLVVQQIGMAMLLALMLFVI
                     FNDFVNIF"
BASE COUNT          444 a          620 c          742 g          457 t
ORIGIN      
        1 ggggattttc gccctgatag gagatcaggc cgatggcgag gaggaggccg aagaggagga
       61 ggagcaggcc gagcagctcc tcgcttttgc gggcatcgag caagggttgg gctgccttga
      121 ccgggctctt tttctttgtg cgtttgcgca tggaacctga gcgtccttcg gaggtccggt
      181 actatttctt gtagaagggg gtttcgcaga ccgtggccgg catgcggctg tttctgatct
      241 cgatctcgag cggggtgccg accgcggcca gttcgaccgg gacgtagccg gtgccgatgc
      301 ccttttcaag gctgggcgag aaagtgccgc tggtgacgac cccgacgcgc tcaccgccgg
      361 agtagatggc atagccgtgg cgcggaaagg cgcgtccggg catgtcgaag ccgatcagtt
      421 tgcgtttgag gccggcggcc ttgatctgaa gcagggcttc acggctgatg aactcgccct
      481 tgttgagttt ggtgatccag ccgaggccgg cctcgagggg attggtggtc tgatcgatgt
      541 cattgccata aaggcagtat ttcatctcga ggcgcagggt gtcgcgtgcg ccgaggccga
      601 tgggttcgat gtcgaactcg gcgccggccg ccatcaccgc tttccagacc ttcacagccg
      661 attcattggg catcatcaac tcatacccga gttcgccggt gtagccggtg cgcgaaatca
      721 tcatcgggac gccggcggcc tcggcctcat ccagccagta atagtcgatt ttgttcaaat
      781 ccagtgccgt gagcttttgc agggtctttt gggccatcgg cccctgcagg gcgagcaggg
      841 aatagcggtc gctctcgttg gtcagggtaa cgccgccgat gaggtgacgg ttggcccatt
      901 cccagtcctt gtccagattg gcggcattga cgaccatcag atagtggtcg gggaagcggt
      961 agatgagcag atcgtcgacg atgcccccct ccgggtagca catcgccgaa tactgcacct
     1021 gacggacgtc caccttgctg acatcgttga tggtgatctt ttgcagaaag gcctcggcat
     1081 tggatccctt gacaatgaat tcgcccatat gggagacgtc gaagaggccg acggtggtgc
     1141 ggacccggcg atgctcctcg atgatgccgc ggtactggat gggcatataa tagccggcga
     1201 actcgaccag cttggcgccc gcctgctggt gcacgtcata taaagcggtt ttcctggttt
     1261 ccacagcgcc tcccgtagca aaaggttcgc aaggttgcga tggtaatatt ctttcaatct
     1321 gaaaattaac atttatttgc gagagttgca agaagtaatt tccgcacaaa aaaaagcccc
     1381 ggtggacgcc ggggctggtc gtgaaaacct agaaaatgtt gacaaaatca ttaaaaatga
     1441 cgaagagcat cagggccagc agcatggcca tgccgatctg ctgaacgacg aggcgcacct
     1501 tggtcgagat cggcttgcgc gtcaccccct cataggcgag caggagcaga tggccgccgt
     1561 cgagagcggg gatgggaaaa aggttgatga tgccgaggtt gaggctgagc agggccatga
     1621 acatcaccag gctgccgaag ccggccttgg cggtctcgcc ggtcatctgg gcgatgcgca
     1681 ccggtccagc cagcccctcc ttgaccgaaa cgtcgccgct gaaaagcatg ccgaaggagc
     1741 ggaacatcat ccgggtcagg ttccaggtcg actgccagcc tacggccatg cccttgaaaa
     1801 agccgggccg cacgttgatg gttttggcgt cgatgccaac cagtccgtag ccgagcatct
     1861 tgtcgagttt gggggtgatc agcgccgaca tcggggcgcc gctgcgctcc cattcgatct
     1921 tgatctcctt ctcgggggag gcgtggatga tgcggatgag gtcgtcccag gaagagatcg
     1981 gggcgccgtc gatgcggaca atgcggtccc cgggctggag gcccgccgtc tgggccggca
     2041 tgttctccat gacgcggccg acgatgggac cgaccggctc ggggatgccg gtgatgaaga
     2101 cgctgacggt gaagacgaag atggtgagca ggacgttggc gagggggccg gcgaaaagga
     2161 ccagcatccg cacccagacc ggcttggaca tgaactccca gggttccccc ttgactcccc
     2221 cggtgtccat tgattcgtcg atcatgccag agagcttgac gta
//
LOCUS       DAOPWU010000482         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_50949_length_2260_cov_4.028118, whole genome shotgun sequence.
ACCESSION   DAOPWU010000482 DAOPWU010000000
VERSION     DAOPWU010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50949_length_2260_cov_4.028118"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2260)
                     /locus_tag="PKM23_15420"
     CDS             complement(<1..>2260)
                     /locus_tag="PKM23_15420"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNY92912.1"
                     /translation="EIDILGLNCATGPEAMRPYIREICQQFPGAVLCQPNAGLPQNVN
                     GEMLYTLAPEEFVHHLGAMVREEGVQLVGGCCGTHPGFIGLLAAELAEVRPPSRRVQP
                     APALSSLFSGVTLRQEPAPFFVGERANTNGSKIFRDFLLAGDWDGMVSVGIEQQRSGA
                     HGIDLCVAYTGRDEIADMREAVSRYAVSVQLPLFIDSTDVRVLEEALQRCGGRAVINS
                     INLEEGEGRADRICALAKRYGAALIALTIDEQGMALEVERKLAVARRLYDIAVGRHGL
                     RPQDLIFDPLTFTLGSGDASLTTAGLSTLEGVRRIKEELPGVYTLLGVSNISFGLAAQ
                     AREILNSVFLAEAVKAGLDLAIVNVKKIIPLYQIAPEDLEIAMNLVYTRGGGDPLLAF
                     IDHFDKKAGVSKEEEGDESLLPLGERIQNRIIQGNRSGLEPMLMEALRSQSALEIINT
                     LLIPAMKHVGDLFGSGRMQLPFVLQSAEVMKFAVDKLQPFMDKSAEQARNAIVLATVR
                     GDVHDIGKNLVDIILTNNGYTVYNLGIKCEIDTMIQKALEVKADAIGMSGLLVKSTVV
                     MKENLEELQRRGLRLPVLLGGAALTRSYVEEICSPLYDAPVVYCADAFEGLTAMGRIQ
                     EKSLTKAAAREPRAARSAAADAPAVISAAIRRDVEIPVPPFWGDRIAGGIDLETVFGY
                     LTENVLFRGRWGYRRGKLSAEEYQALLEEQVKPEFRALKERCIAEKLLEPKVAYGYYP
                     CSSCGEEVILYHP"
BASE COUNT          376 a          675 c          802 g          407 t
ORIGIN      
        1 ggatggtaga ggatcacctc ctccccgcag ctgctgcagg gatagtagcc ataggccact
       61 ttgggctcga gcagtttttc ggcgatgcag cgctccttga gagccctgaa ttcgggcttg
      121 acctgttctt caagcagggc ctggtactcc tcggcagaga gtttgccgcg ccggtagccc
      181 cagcggccgc ggaagagaac attctcggtc agataaccga agacggtctc gagatcgatc
      241 cccccggcga tgcgatcgcc ccagaagggg gggacgggga tttcgacatc ccggcggatg
      301 gctgcgctga tcaccgccgg cgcgtctgcg gccgcactcc tggccgctcg cggctccctg
      361 gccgcggctt tggtcaggct cttctcctga atccggccca tggcggtcag cccctcaaag
      421 gcatcggcgc agtagaccac cggggcgtca tagaggggcg agcagatctc ctcaacgtag
      481 ctgcgggtga gggcggcccc gcccagcagc accggcaggc gcagaccgcg gcgctggagc
      541 tcctcgaggt tctccttcat caccactgtc gatttgacca gcaggccgct catgccgatg
      601 gcgtcggcct tgacctccag ggccttctgg atcatggtgt cgatctcgca cttgatgccg
      661 agattgtaga cggtgtagcc attgttggtg aggatgatgt cgacgagatt cttgccgatg
      721 tcgtgaacgt cgccgcgcac ggtggcgagg acgatggcgt tccgggcctg ctctgcactc
      781 ttgtccatga agggctggag tttgtctacg gcgaacttca tcacctcggc cgactggagg
      841 acgaagggga gctgcatacg gccgctgccg aagagatcgc cgacgtgctt catggcgggg
      901 atgagcaggg tgttgatgat ctcgagggcg ctctggctgc gaagggcttc catgagcatg
      961 ggttcgaggc cggaacggtt gccctggatg atgcggttct gaatgcgctc gccgaggggg
     1021 aggaggctct cgtccccttc ctcctccttg ctcacgcccg cctttttatc aaaatggtcg
     1081 atgaaagcga ggaggggatc acctccacca cgggtataga ccagattcat ggcgatctcg
     1141 agatcctccg gggcgatctg gtagagggga atgatctttt tgacgttgac gatggccaga
     1201 tcgagccccg ccttgaccgc ctcggcgaga aagaccgaat tgaggatctc gcgggcctgg
     1261 gcggcgaggc cgaaggagat gttgctcacc ccgagcaggg tgtagactcc cggcagctcc
     1321 tccttgatgc ggcgcacccc ttcgagggtg gagaggccgg cggtggtgag cgaggcgtca
     1381 ccgctgccga gggtgaaggt gagggggtca aagatgagat cctgcggccg caggccgtga
     1441 cggccgacgg cgatgtcgta gaggcgacgg gctacggcga gcttgcgctc gacctcgagg
     1501 gccatgccct gctcgtcgat ggtgagggcg atgagggcgg cgccgtagcg tttggccagg
     1561 gcgcagatgc ggtcggccct gccctcgccc tcctcgaggt tgatcgagtt gatcaccgcc
     1621 cgtccgccgc agcgctgcag ggcttcctcg aggacccgaa catcggtgga gtcgatgaag
     1681 aggggcagct ggacgctgac ggcgtagcgg ctgaccgctt cgcgcatgtc ggcgatctcg
     1741 tcgcgcccgg tgtaggcgac gcagaggtcg atgccgtggg ccccgctgcg ctgctgttcg
     1801 atgccgaccg agaccatgcc gtcccagtcc ccggcgagga gaaagtcgcg aaagatcttg
     1861 ctgccattgg tgttggcccg ttcgccgacg aagaaggggg caggttcctg gcgcagggtc
     1921 accccgctga agagggagga gagggcgggc gccggctgga cccggcggga cgggggcctc
     1981 acctcggcca gctccgcggc gagcagaccg ataaatccgg ggtgggtgcc gcagcagccg
     2041 ccgaccagct gcaccccctc ctcacgcacc atcgcgccga ggtggtgaac gaactcttcc
     2101 ggggcgaggg tgtagagcat ctcgccattc acgttttgag ggaggccggc attgggctgg
     2161 cagagcaccg cgccggggaa ctgctggcag atctcgcgga tgtagggccg catcgcctcg
     2221 gggccggtgg cgcagttgag gccgaggatg tcgatttcaa
//
LOCUS       DAOPWU010000483         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_51377_length_2248_cov_4.326037, whole genome shotgun sequence.
ACCESSION   DAOPWU010000483 DAOPWU010000000
VERSION     DAOPWU010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51377_length_2248_cov_4.326037"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKM23_15425"
     CDS             complement(<1..630)
                     /locus_tag="PKM23_15425"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNY92913.1"
                     /translation="MIASFEDLLRLVTGQVKRTIAVAMAEDEDILEALCLAYSRGIAD
                     AVLVGNKTAILEMARKGSLDLSPFDIIQADSEQQSVSVAIQLVRQQMANALMKGKCTT
                     ATLLKGVLDKEAGLRAGKLLSHLAAFTVPAYHKLLLMSDAAMNIAPDLAGKIAITENA
                     VHTAHQLGIAQPRVAMIAAVEKVNHDSMPCTVDAAALAQMGARGQIKGAI"
     gene            complement(627..1067)
                     /locus_tag="PKM23_15430"
     CDS             complement(627..1067)
                     /locus_tag="PKM23_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006512484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNY92914.1"
                     /translation="MIVVDSQLIAALLLNNEKTDLAREVLRRDDEWIAPLLWRSEFRQ
                     LLAFYLRKKVLTLRQASLIMQEAESLMQGGQFEIPSLDILTVTEKYDCSAYAAEYIAL
                     AQEQGVPLVTMDKMYLRKFPAVTMNAESFISMKITQEQVHPEML"
     gene            complement(1064..1306)
                     /locus_tag="PKM23_15435"
     CDS             complement(1064..1306)
                     /locus_tag="PKM23_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HNY92915.1"
                     /translation="MQTITVKKIPPDLYKKLKQLAKENRRSINSEIIVCIERAICSQR
                     IDSDTFLKRIEQLQKGLDLPAIAQSGLEKARDEGRS"
     gene            complement(1471..>2248)
                     /gene="buk"
                     /locus_tag="PKM23_15440"
     CDS             complement(1471..>2248)
                     /gene="buk"
                     /locus_tag="PKM23_15440"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNY92916.1"
                     /translation="KADFGEHASNLGAILAHELAAMYGRPAFIVDPVVVDELEPEARL
                     TGHPMLPKQCIFHALNHKAVGRKAAGQLGKPYEALNLIIAHLGGGISVAAHQHGRVVD
                     VNNALNGDGPFSPERSGTLPAGALVRLSFSGEYSEREISRMITGKGGVVAYLGVNDMR
                     LVTEMIRAGDAKALLVYRAMAGQVAKEIGSMAAVLSGEVDAVVLTGGIAYDEEFTRLI
                     SMRVGFIAPVLLFPGEEEMEALALGGMRVLRGEEQAREYD"
BASE COUNT          477 a          634 c          655 g          482 t
ORIGIN      
        1 aattgcgccc ttgatttggc cgcgagcacc catctgagcg agagcggcag catccaccgt
       61 gcagggcatg gagtcgtgat tgaccttttc caccgccgcg atcatcgcca cccggggctg
      121 cgcgatgcca agctgatggg cggtgtggac ggcgttctct gtgatggcga tcttgccggc
      181 aaggtcagga gcgatattca tggccgcatc tgacatcagg aggagcttgt ggtaggcggg
      241 gacagtgaaa gcagcgagat ggctgaggag tttgccagcg cgcaagcctg cctccttgtc
      301 gagcacaccc ttgagcaggg tggcggtggt gcacttgcct ttcatcagcg cgttggccat
      361 ttgctggcgc accaactgta tggctacaga aaccgattgc tgttcgctgt cagcctggat
      421 gatatcaaag ggagagagat caaggctgcc cttccgggcc atttcgagga tggcggtttt
      481 attgccgacc aggacggcat ccgcgatgcc ccggctgtag gcaaggcaaa gggcttcgag
      541 gatatcttca tcctcggcca tcgcgacggc aatggtccgt ttgacctgtc cggtgacgag
      601 acgaagcaaa tcctcgaacg aagcgatcat agcatctccg gatggacctg ttcctgagtg
      661 attttcatgg aaataaaact ttcagcattc attgtaaccg ctggaaactt gcgtagatac
      721 atcttatcca tagtgacaag cggcaccccc tgttcctggg cgagggcgat atattccgcg
      781 gcataggcgg agcagtcata tttttcagtc acggtgagaa tgtccaggga gggaatttcg
      841 aactggccgc cctgcatgag gctctcagcc tcctgcatga tcaggctggc ctggcggagg
      901 gtcaggactt ttttacgcag gtagaaggcg agcagctgac ggaactcact gcgccacaac
      961 agcggcgcga tccattcatc atccctgcgc aggacctccc gggccagatc ggttttttca
     1021 ttgttgagca ggagtgcggc gatcagctgt gaatcgacca caatcatgag cgtccctcat
     1081 ccctcgcctt ttcaagaccg gattgggcga tggcaggcag gtccaatccc ttttgcaatt
     1141 gttcgatccg cttcagaaag gtgtccgaat caatccgctg gctgcaaatc gcgcgctcta
     1201 tacacacgat gatctcgctg ttgatgctgc ggcgattttc cttggcgagc tgcttgagct
     1261 ttttgtagag gtcgggaggt atttttttaa cggtgatggt ctgcattaaa accactcctg
     1321 aacggttctg atttggaacc ataatggaat atacgacatt ttgcagcatt tgtcaagtaa
     1381 aaattgaagt taacgcataa aaaacggccg gacattttca gttccagccg ttcagatcgt
     1441 tgcagggagc gaatggacac cgcagccagt ctagtcgtac tccctggcct gctcctcacc
     1501 gcgcagcacc cgcatcccac ccagagccag ggcctccatc tcctcctccc cggggaaaag
     1561 gagcacggga gcgataaaac caacacgcat gctgatgagt ctggtgaact cttcatcata
     1621 ggcaatgccg ccggtgagga caacggcatc cacctctcca ctcagcacgg cggccatgct
     1681 gccgatctcc ttggcgactt gtccagccat ggcgcggtag acgagcaggg ctttggcatc
     1741 ccccgcccta atcatctctg tgaccaggcg catgtcgttg acgcccagat aagcgaccac
     1801 cccacccttt cccgtgatca tacggctgat ctcgcgctca ctgtattcgc ctgagaagga
     1861 gagcctgacc agagcgccgg ccggcagggt gccactgcgc tccggagaga aaggcccgtc
     1921 gccgttaagg gcattgttga catcaacgac ccggccgtgc tgatgggcgg ccacggagat
     1981 accgccgccg agatgagcaa taatcagatt gagcgcttca tagggtttgc ccaactgccc
     2041 ggccgcctta cggcccacgg ccttgtgatt gagggcgtga aagatgcact gcttgggcag
     2101 catgggatga ccggtgagac gcgcctcggg ttccagttca tcgacgacca caggatcgac
     2161 gataaaggcc ggccggccgt acatggcggc caactcatgg gcgaggatgg cgccgagatt
     2221 ggaggcgtgt tcgccaaagt cggccttc
//
LOCUS       DAOPWU010000484         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_51695_length_2238_cov_4.072836, whole genome shotgun sequence.
ACCESSION   DAOPWU010000484 DAOPWU010000000
VERSION     DAOPWU010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51695_length_2238_cov_4.072836"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1494
                     /locus_tag="PKM23_15445"
     CDS             172..1494
                     /locus_tag="PKM23_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNY92917.1"
                     /translation="MDSASPRAKRPLHIKIFIGLAVGLFAGLIVNFFWGESATLDWLI
                     RNLIAPVGQLFLRIIFMAVIPLIFSALALGVAELGDIRQLGRIGARTLGYTVIVTLIS
                     VLIGLFLVNWIQPGTGLSEAERQMLTGSLTVSSVNKTVQSAAEAKSVVQTLLDLIPKN
                     PLADAVGAFDENYRGGGILAVMVFSLFVGLALTLTRSERTETFERFLQGLYDVVMIII
                     HMGMKLAPLGVACLVFSVVARLGLDVVVILGKFVFVVMAGLLIHLLGVYSLILKYRIK
                     VSPWRFFGRIKEVILTAFSTSSSNVTLPTALRTADQNLGIPRHIGNFVLTLGSTANQN
                     GTALYEGVTVLFLAQFFGVELTLVQQLTVIFAAVLAGIGTAGVPGGSLPVIVLILQSV
                     GVPGEGIGIIFGVDRILDMSRTVINVTGDLTAAMYVARAEGYELELES"
     gene            1544..2236
                     /locus_tag="PKM23_15450"
     CDS             1544..2236
                     /locus_tag="PKM23_15450"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNY92918.1"
                     /translation="MEFPYRIIDLSHPLQPGMPVWPGDPPLAITPAAGLDREGYYLNR
                     LTLGEHTGTHAGAPAHYLAGGPAMDAIPVADLVAPLFTIAVPLQGDALLEPADVAEWE
                     ARFGPLPPGCAVVVATGWSRHWSDEARYLGRAADTSLHFPGISPAAMGLIAGVRGARI
                     VGIDSPGLDGGLSADYAAGKLLARRGGLHLENLTALELLPPFGAWLVAGLLPIAGGSG
                     SPARVLALVPRA"
BASE COUNT          334 a          755 c          674 g          475 t
ORIGIN      
        1 atgtagcggt gggagcgaga attggcggcg ggagcgagag agattgcccg gatgggagcc
       61 agatttcggg gggtaccctg ctgaaatttg accttgcatt tcctcatgct gtttcctaaa
      121 ttgcgcgaaa caagctgcgc ttctgctccc actgattccg aggagatggt catggattca
      181 gcctcacccc gcgccaaacg tcccctgcac atcaaaatct tcatcggtct ggcggtcggc
      241 cttttcgccg gattgatcgt caattttttc tggggtgaga gtgcgacgct cgactggctc
      301 atccgcaatc tcatcgcccc cgtggggcag ctctttttgc gcatcatctt catggcggtg
      361 attccgctga tcttttcggc cctcgccctg ggggttgccg aactggggga tatccgtcag
      421 ctcggccgca ttggcgcccg caccctcggc tataccgtga tcgtgaccct gatctcggtc
      481 ctgatcggcc tgttcctggt caactggatt cagcccggca ccggcctgag cgaggcggag
      541 cggcagatgc tgaccggttc tctcaccgtc tcctcagtca acaagacggt tcagagcgcg
      601 gcggaggcca aatcggtggt gcagaccctg ctcgatctta ttcccaagaa tcccctcgcc
      661 gacgcagtcg gggcctttga cgaaaactat cgcggcggcg gcatcctggc ggtgatggtt
      721 ttctcccttt ttgtcggact ggccctgacc ctgacgcgtt cggagcgcac cgagaccttc
      781 gaacgttttt tgcagggtct ttatgatgtg gtaatgataa tcatccatat ggggatgaaa
      841 ctggcccccc tcggtgtcgc ctgcctggtc ttcagcgtgg tcgcccgact cgggctcgat
      901 gtagtggtga tcctcggcaa gtttgtcttt gtggtcatgg ccggactgct catccatctc
      961 ctcggggttt actcgctgat cctgaaatac cgcatcaagg tcagtccctg gcgcttcttc
     1021 ggccgcatca aggaggtcat cctcaccgct ttttcgacca gttcgagcaa cgtcaccctg
     1081 cccacggccc tgcggactgc cgaccagaac ctgggcatcc cgcgccacat cggcaacttt
     1141 gttctcaccc tcggctccac cgccaatcag aacggcaccg ccctctacga gggggtgact
     1201 gtccttttcc tcgcccagtt tttcggggtc gagctgaccc tggtgcagca gctcaccgtc
     1261 atcttcgctg cagtgctggc cgggatcggc acggccgggg tgccaggcgg ttccctgccg
     1321 gtcatcgttc tcatcctcca gtctgtcggc gtccccggcg aaggcatcgg catcatcttc
     1381 ggggtcgacc gcatccttga tatgtcccgc acggtgatca atgtcaccgg cgatctcacg
     1441 gcagcgatgt atgtcgcccg cgccgagggc tatgaactgg agctggagtc atgaccgcct
     1501 gcggcgacag gttgactgcc cttgcggaga ggcggagcgt ctgatggagt tcccttaccg
     1561 gatcatcgat ctgagccacc ccctccagcc cggcatgccg gtctggcccg gggatccgcc
     1621 cctcgccatc actcccgccg cgggcctgga tcgggagggc tattatctca accgcctgac
     1681 cctgggtgaa cacaccggaa cccatgccgg agcgccagcc cattatctcg cgggcggacc
     1741 ggccatggac gccattcctg tagctgatct cgtcgcccct cttttcacca tcgcagtgcc
     1801 cctgcagggc gatgcattgc tcgagcccgc cgacgtggcg gagtgggaag cgcgctttgg
     1861 ccctctcccg cccggctgcg cggtggtggt tgcgaccggc tggagccgcc actggtccga
     1921 tgaagcgcgc tatctggggc gcgcggccga caccagcctg cacttccccg gcatcagccc
     1981 ggcggcgatg ggactcatcg ccggggtgcg cggggcgcgc atcgtcggca tcgacagccc
     2041 cggcctcgac ggcggcctct ccgccgacta tgcggcggga aagctgctgg cgcgccgcgg
     2101 cggcctccat ctcgaaaacc tcaccgccct cgagctgctg cccccgttcg gggcctggct
     2161 cgtagccggc ctcctgccca tcgccggggg cagcggctcc cccgcacgcg tgctcgccct
     2221 ggttccgcgc gcctgatg
//
LOCUS       DAOPWU010000485         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_51726_length_2237_cov_4.898258, whole genome shotgun sequence.
ACCESSION   DAOPWU010000485 DAOPWU010000000
VERSION     DAOPWU010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51726_length_2237_cov_4.898258"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PKM23_15455"
     CDS             complement(<1..250)
                     /locus_tag="PKM23_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002791718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNY92919.1"
                     /translation="MTLLDYGIVIAYLVGVLIIGLWSERRAKQNLDAYFLGNRSLPWW
                     ALGASGMASNLDVSGTMINTAFIFALGATGFFIEIRGGV"
     gene            complement(267..>2237)
                     /locus_tag="PKM23_15460"
     CDS             complement(267..>2237)
                     /locus_tag="PKM23_15460"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY92920.1"
                     /translation="ALLLLTPVLFFLLLEGGLRLFRYGGNDALVLLREDYGERYYQIN
                     RRVAQRYFSSDEVNVPDARGEAFAFHKTPNTYRIFCLGGSTTAGWPYQQNAGFPSQLQ
                     ARLSLLFPERNFEVINVGISAINSYSVLDFTGELLRYQPDLFLIYMGHNEFYGALGAG
                     STRSIALGRPFIKAYMRLERLRLVQLLRNGAAQLRRKPPAAGGHETLMENMAGDRMIL
                     PESPRYRRARADFAANLDEILTRIAKKGVPVLVSTLVSNIADQPPFESPFSPNFSRQA
                     EWRALEEQGDRALAGGEPAAALAFYRQAAVLDSLPAMLSYKMGQCHRARGDSLRARRL
                     LERARDLDGLRFRASAEFNQVIRRVCAGKSVPVVETENHFINASPGGLIGWSLMHDHL
                     HPNAFGYLLMADAFCRTMAEADLIVDSSAWPWNRDLPAERLLEEAAITPFEEEIACQR
                     IRALTSRYPFRAPRLLQAVDEPEYARMLQGAVQMLFQRKWSWNEAHYRVGDWLAERGR
                     HAEAAQEYRAVIRVMPGNYYPYLFLANMLALQGKGEAEERILLQAAALSQNLPFAFAK
                     LGVHYMGRNDGRKARPVLERAIALADNSRDFTPTDRSRVHYLLGVALAQEGDYAAAER
                     EAETALQLAPGEARILNLLQQLAEVMPPSTNP"
BASE COUNT          416 a          664 c          776 g          381 t
ORIGIN      
        1 tgacgccgcc gcggatctcg atgaaaaagc cggtggcgcc gagggcgaag atgaaagcag
       61 tgttgatcat ggtgccgctg acgtcgaggt tggaggccat gcccgaagcc ccgagggccc
      121 accagggcag ggagcggttg ccaaggaaat aggcatccag gttctgcttg gcgcgccgtt
      181 cgctccagag gccgatgatg aggacgccga cgagataggc gatgacgatg ccgtagtcga
      241 gtagggtcat aggtgtttcc ttctcgttag gggtttgttg atggtggcat gacctctgcc
      301 agctgctgta aaaggttgag gatccgcgcc tcgcccggcg ccagctgcag ggcggtttcg
      361 gcctcccgct ccgcggccgc atagtcgccc tcctgagcca gggccacccc gagcagatag
      421 tgcacccggg agcggtcggt gggggtaaaa tcacggctgt tgtccgccag ggcgatggcc
      481 ctctccagaa ccggccgggc cttgcggccg tcattccggc ccatatagtg cacacccagt
      541 ttggcgaaag caaaaggcag attctggctc agggccgcgg cctgaagaag aatcctctcc
      601 tccgcctccc ccttgccctg cagggcgagc atgttggcga ggaagaggta ggggtaataa
      661 ttgcccggca tgacacggat gaccgcgcgg tactcctgcg ccgcctcggc atgccggccc
      721 cgttccgcca gccagtcgcc gacgcggtag tgggcttcat tccagctcca tttgcgttgg
      781 aagagcatct gcaccgcccc ctgcagcatt ctggcatatt ccggctcgtc gaccgcctgg
      841 agcaggcggg gtgcgcggaa gggatagcgg ctggtgaggg cgcggatgcg ctggcaggcg
      901 atctcctcct caaagggggt gatggcggcc tcctcgagga gccgctccgc cggcagatcg
      961 cggttccacg gccatgcaga ggagtcgacg atcagatccg cctccgccat ggtccggcaa
     1021 aaagcatccg ccatgagcag ataaccgaag gcgttggggt gaagatggtc gtgcatgagg
     1081 gaccagccga tcaggccgcc cggggaggcg ttgatgaaat ggttttcggt ctcgaccacg
     1141 ggcacacttt ttccggcgca aaccctgcgg ataacctgat tgaattctgc actggcgcgg
     1201 aagcgcagtc catcgagatc gcgggcgcgc tcgagcagcc tgcgggccct gagcgagtcc
     1261 ccccgggcgc ggtgacactg ccccatcttg taggagagca tcgccggcag ggagtcgagc
     1321 actgcggcct gccgataaaa ggcgagggca gcggcaggct ctccgccggc gagggcccgg
     1381 tcgccctgct cctcgagggc gcgccactcc gcctgccggg aaaagttggg ggaaaagggg
     1441 gattcgaagg ggggctggtc ggcgatgttg ctgaccaggg tgctcaccag caccgggacg
     1501 ccctttttag cgatcctggt gaggatctcg tcaaggttgg cggcgaaatc ggcccgggcg
     1561 cggcggtagc gggggctttc aggcaggatc atccggtctc cggccatatt ttccatcagg
     1621 gtctcgtgcc caccggccgc cggcggcttg cggcgcagct gtgcggctcc gtttcgcaag
     1681 agctgcacca gccgcagccg ctcgagccgc atataggcct tgatgaaggg acggccgagg
     1741 gcgatactcc gggtgctgcc ggcgccgagg gcgccgtaga actcgttgtg ccccatatag
     1801 ataagaaaaa ggtcgggctg atagcggagc agctcgccgg tgaaatctag gaccgaatag
     1861 ctgttgatgg ccgagatgcc gacgttgatc acttcaaagt tgcgctccgg gaagagcagg
     1921 ctgagccggg cctgcagctg actgggaaag ccggcgtttt gctgataggg ccagccggcg
     1981 gtggtggaac cgcccaggca aaaaatgcga taggtgttgg gagttttgtg gaaagcaaag
     2041 gcctccccgc gcgcgtccgg aacattgacc tcgtcgctgc tgaaatagcg ctgcgccacc
     2101 cggcgattga tctggtagta acgctcgccg tagtcctcac gtaacagcac cagagcgtcg
     2161 ttcccgccat agcggaagag ccgcagcccc ccctcgagca ggaggaaaaa gagaacaggt
     2221 gtcaggagca gcagggc
//
LOCUS       DAOPWU010000486         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_52406_length_2218_cov_3.822006, whole genome shotgun sequence.
ACCESSION   DAOPWU010000486 DAOPWU010000000
VERSION     DAOPWU010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52406_length_2218_cov_3.822006"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PKM23_15465"
     CDS             <1..890
                     /locus_tag="PKM23_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNY92921.1"
                     /translation="IALYRQGRIVGGVYTGRGVEATTVGSGLALEAQDWLFPIHRDLG
                     AHLAKGQSVLNILLQYLGRGNGLSRGKDSGIHCSDTRLNIVGNVSHLGHMIPVAVGTA
                     IGKRMQGEKVVALNFIGEGGSNIGDFHEGLNFAAVQKAPFVLIIENNHYAYSTPTHKG
                     FSAKYLSDRAIGYGIPGHHVDGTDVLQVYKVVKEAVERARAGEGPSLIECLSFRMVGH
                     SAHDDAFYVDKQRMKEYEKKDPILRLEHHLHREGTLSEPLIATIKAELEEEIEQATQA
                     ALDSPLPEAHWAAEGLFAE"
     gene            907..1887
                     /locus_tag="PKM23_15470"
     CDS             907..1887
                     /locus_tag="PKM23_15470"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNY92922.1"
                     /translation="MAETTYLQAITDAMREEMLRDPRVFLLGEDIGVYGGAFKVTKGL
                     IDEFGEERVMDTPISEAAIVGVAVGAALTGMRPIAEMQFADFITSGFSQLVTNAATLH
                     YRYGTAVPMVVRAPSGGGIHGGAFHSKNPEAWFVHQPGLKVVAPATARDAKGLLKSAV
                     RDDNPVLFFENKYLYRRIKDEIDPGEELLVPLGRAHIARAGGDVSIITYGAMVHAAQE
                     AADKLAAEEGVEAEIIDLRSLQPLDKEAIFAAVMRTNRVVIVHEANLTGGIGGEIAAL
                     ISEHAFEALDAPIKRVAGLDTPTPYAPAMEEFWRPNAEKIAQAIREVIRF"
     gene            1905..2150
                     /locus_tag="PKM23_15475"
     CDS             1905..2150
                     /locus_tag="PKM23_15475"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNY92923.1"
                     /translation="MTNVEQEKLLSMREILLTAIKREEEAYTFYISARQCARTTTEEE
                     MFTRLAAQEMQHKRALEIQLEEIDSQLEIDRALSYDI"
BASE COUNT          467 a          709 c          633 g          409 t
ORIGIN      
        1 tcattgccct ttaccgccag gggcgcatcg tcggcggggt ctacaccggc cgtggggtgg
       61 aagcgaccac agttggttcc ggcctcgccc tcgaggccca ggactggctc ttcccgattc
      121 accgcgatct gggcgcccat ctggccaagg gacagagcgt gctcaatatc ctgctgcagt
      181 atctcggcag gggcaacggc ctctcgcgcg gcaaggacag cggcatccac tgcagcgata
      241 cacgcctcaa catcgtcggc aacgtcagcc atctcggcca tatgatcccg gtcgccgttg
      301 gcaccgccat cggcaagcgc atgcaggggg agaaggtggt ggctctcaat ttcatcggcg
      361 agggcggttc caatatcggc gatttccacg aggggctgaa tttcgctgcc gtacagaaag
      421 ctcccttcgt cctcatcatc gaaaacaatc attacgccta ctcgactccc acccacaagg
      481 gcttctccgc caaatatctc tccgaccgcg ccatcggcta tggcattccc ggccatcacg
      541 tcgacggcac cgatgtcctg caggtctaca aggtcgtgaa ggaggcagtc gaacgcgccc
      601 gcgccggtga ggggcccagt ctgatcgaat gtctcagctt tcgcatggtg ggccattcag
      661 cccatgacga cgctttttac gtcgacaagc agcgcatgaa agagtatgaa aaaaaggatc
      721 ccattctgcg tctcgagcat cacctccaca gggaggggac cctcagcgag ccactgatcg
      781 ccactatcaa ggccgaactc gaggaggaga ttgaacaagc cacccaggct gcgctcgaca
      841 gcccgcttcc cgaagctcat tgggccgccg aaggcctttt cgctgaataa tccgaaggaa
      901 acccttatgg ccgaaacaac ctatttgcaa gccatcaccg acgccatgcg cgaagagatg
      961 ctgcgcgatc cccgggtctt tctgctcggc gaggacatcg gcgtctacgg cggtgctttt
     1021 aaagtgacca agggattgat cgatgaattc ggcgaggaac gggtgatgga caccccgatc
     1081 tccgaagccg ccatcgtcgg cgtggctgtc ggcgccgccc tcaccggcat gcgtccgatc
     1141 gcagagatgc agttcgcaga ttttatcacc tcgggcttca gccagctggt caccaacgct
     1201 gccaccctgc actaccgcta cggcacggcc gtgcccatgg tggtccgcgc cccctcggga
     1261 ggcggcatcc atggcggggc ttttcactcc aagaaccccg aagcctggtt tgtccaccag
     1321 cctggactca aggtcgtcgc tcccgccacc gcgcgggacg ccaaggggct actcaagagt
     1381 gcggtgcgcg atgacaatcc ggtcctcttt ttcgagaaca agtacctcta tcgccgaatc
     1441 aaggatgaaa tcgatcccgg cgaggagctc ctcgtcccgc tcggcagggc ccatatcgcc
     1501 cgcgccggcg gtgatgtctc gatcatcacc tacggagcca tggtgcacgc tgcgcaggag
     1561 gccgcagaca aactggcggc cgaagagggc gtggaggccg agatcatcga tctgcgttct
     1621 cttcaacccc tggacaagga ggccatcttc gcagcggtca tgcgcactaa ccgcgtcgtc
     1681 atcgtccacg aggccaacct caccggaggc atcggcggag agatcgccgc cctgatctcg
     1741 gaacacgcct ttgaggccct cgatgccccg atcaagcgcg tcgccggcct ggatactccg
     1801 accccctatg ccccggcgat ggaggagttc tggcgtccga acgcggaaaa aattgcccag
     1861 gccatccggg aggtcatccg tttttaatca gcaggagtgc caccatgact aacgtagaac
     1921 aggaaaaatt gctctctatg cgggagatcc ttctcaccgc catcaagcgt gaggaggagg
     1981 cctacacctt ttacatcagt gcgcggcagt gcgcgcgcac aaccaccgag gaggagatgt
     2041 tcacccgtct tgccgcccag gagatgcagc ataaacgggc cctcgagatc cagctggagg
     2101 agatcgacag ccaactggaa atcgatcgtg ccctttcgta cgacatctga aacgaattga
     2161 tgaaaggttg atatatgaaa gggcacgatc gatttccagt tggctgtcga tctcctcc
//
LOCUS       DAOPWU010000487         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_52476_length_2216_cov_4.158723, whole genome shotgun sequence.
ACCESSION   DAOPWU010000487 DAOPWU010000000
VERSION     DAOPWU010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52476_length_2216_cov_4.158723"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKM23_15480"
     CDS             <1..695
                     /locus_tag="PKM23_15480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92924.1"
                     /translation="VFEYTGAMTLNYPAVSAASDSAGTPGSPLFLRRLDNSEAWLAFA
                     GGDGLVQAFNQHGEHQPGFPLSGGISAESALLLGDVDGDGILEMAAAGNNGSLNLWDL
                     GTASSTCTVWGEAGGAQRTFALAGASVTPVPGSDLMLAKKVFSYPNPVTEGAANIRFT
                     LARPVERVLVRIFDLAGNLVGELSRENLDAGDHEMAWEVSKVQSGVYFARVTAQSGSD
                     SRFEIIKIAVTH"
     gene            824..>2216
                     /locus_tag="PKM23_15485"
     CDS             824..>2216
                     /locus_tag="PKM23_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HNY92925.1"
                     /translation="MRWTGFILVFCCYAAPILHAQEEPINHPELDWYTLRTDHFQVHF
                     HEGAERTARIVAKVAEEIYEPITSLYNYRPDGAIHFIIKDYDDNSNGAAYYYDNKVEI
                     WAPQMTFILRGTHDWLRNVVTHEFSHMISLGAARKLPRKIPAFYLQAFGYEPEKRDDV
                     LYGYPNVIASYPVAMTSVPMWMAEGMAQIQTPGLQYDLWDSHRDMLIRTAVVAGEQLT
                     YDEMGVFGKNSLGNERTYNSGYALIRYIVRQHGQEALQKLADGLSRPLAFTAESAIRA
                     ATGRGGEVLYREWQEEMGRYYRQRLAVIDSHRVEGEILTPKGMGNTFAAWSPDGRKLA
                     YCGSESSDYLSMTHLWLYDTATGKKKLLKRGVSSQISWSRDSNSLFYARIERGRHGSR
                     LSDLFRYDLQSGKEKQLTRRMRAFGPDLSPDGSTLLCAVQKDGTDNLALLGSDGKHLR
                     DLTAFSNGEGIMGP"
BASE COUNT          438 a          697 c          654 g          427 t
ORIGIN      
        1 ccgttttcga gtacaccggc gccatgaccc ttaactatcc ggccgtttca gccgccagcg
       61 atagcgccgg cacgccgggc agcccactct tcctgcgccg gctggacaac agcgaggcct
      121 ggctcgcctt cgccggcggt gacggtctgg ttcaagcctt caaccagcac ggcgaacatc
      181 agccgggatt tcccctcagc ggcggaatat cggctgagag cgccctgctc ctcggggatg
      241 tggatggcga cggcatcctc gaaatggccg ccgccgggaa caacggcagc ctcaacctct
      301 gggacctcgg cacggcaagt agcacctgca cggtgtgggg agaggcgggt ggagcgcagc
      361 gcaccttcgc actggcgggc gcctccgtga ccccggttcc gggttcagat cttatgcttg
      421 cgaaaaaagt cttttcgtat cccaacccgg tgactgaagg tgccgccaat atccgcttca
      481 ccctcgcccg ccctgtcgaa cgggtcctcg tccgcatttt tgacctcgcc ggcaaccttg
      541 tcggggaact ctcgcgcgag aatctggatg ccggcgacca cgaaatggcc tgggaggtga
      601 gcaaggtgca gagcggggtc tatttcgccc gggtgaccgc ccagtccggg agtgatagcc
      661 gtttcgagat catcaaaatt gctgtcacac attgatgcgg ttaatccaga gacgcaagct
      721 ggctttcctg tccgctgatc aggaaaggac gctcctgcac gccgagttta cgaattatgc
      781 caggatctac tcttttatga gatggagtaa attacgatat ttcttgcgat ggacgggatt
      841 cattcttgtc ttttgctgct acgctgcgcc aatcctccat gcccaggagg agccgatcaa
      901 ccatcccgaa ctggattggt acaccctcag gactgaccat tttcaggtcc attttcatga
      961 gggggccgaa cgcaccgccc gcatcgtcgc caaggtggcg gaagagatct acgaacccat
     1021 taccagtctc tataactacc gtccggacgg cgccatccat ttcatcatca aggattacga
     1081 cgacaactcc aacggcgccg cctactatta tgacaacaag gtggagatct gggcgccgca
     1141 gatgaccttc attctgcgcg gcacccacga ttggctgcgc aatgtcgtca cccacgagtt
     1201 ttcccacatg atctccctcg gggccgcgcg caagctgccg cgcaaaatcc ccgcctttta
     1261 cctgcaggcc ttcggctatg agccggagaa gcgcgacgat gtgctctatg gttatcccaa
     1321 cgtcatcgcc tcttatccgg tggccatgac ctcggtgccg atgtggatgg cggaggggat
     1381 ggcccagatc cagactcccg ggctgcagta cgacctgtgg gacagccatc gcgatatgct
     1441 catccgtacg gcggttgtgg ccggtgagca gctgacctat gacgaaatgg gggtttttgg
     1501 caagaacagc ctcggcaacg agcgcaccta caacagcggc tatgccctca tccgctatat
     1561 cgtgcgccag catggccagg aggccctgca aaaactcgcg gacggcctct cgcggccgct
     1621 ggccttcacg gccgagagtg caatcagggc ggccacaggc agggggggcg aggtcctcta
     1681 cagggagtgg caggaggaga tggggcgcta ttatcgtcag cgcctggcgg tgatcgattc
     1741 ccatcgcgtc gagggggaga tcctcacccc caagggaatg ggcaatacct ttgcggcctg
     1801 gtcgccggac ggccgcaagc tcgcctactg cggcagcgag agcagcgatt atctcagcat
     1861 gacccatctc tggctttatg acaccgccac cggcaagaaa aagctgctca aacgcggcgt
     1921 cagcagccag atctcctgga gccgcgacag caactcgctc ttctacgccc gcatcgagcg
     1981 cggccgtcac ggctcacgcc tgagcgatct tttccgctac gatctgcaga gcggcaagga
     2041 gaaacagctc accaggcgga tgcgcgcctt tggtccggat ctttcgcccg acggcagcac
     2101 tctgctctgt gcggtgcaga aggatggcac cgacaacctc gcgcttctcg gcagcgatgg
     2161 caagcatctg cgcgatctca ccgccttcag caatggcgag gggattatgg ggccgc
//
LOCUS       DAOPWU010000488         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_52688_length_2211_cov_2.884045, whole genome shotgun sequence.
ACCESSION   DAOPWU010000488 DAOPWU010000000
VERSION     DAOPWU010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52688_length_2211_cov_2.884045"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PKM23_15490"
     CDS             complement(<1..448)
                     /locus_tag="PKM23_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNY92926.1"
                     /translation="MSKTKYVYSFGGGKAEGTAKMKELLGGKGANLAEMANLSIPVPA
                     GFTITTEMCTYYYAHGKKNPPELVKDVEAALAAVEKIMDARFGDVQDPLLLSVRSGAR
                     SSMPGMMETVLNVGLTSKTIKGLIKKTGNERFAYEAYRRLIMMYSDV"
     gene            complement(484..>2211)
                     /locus_tag="PKM23_15495"
     CDS             complement(484..>2211)
                     /locus_tag="PKM23_15495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNY92927.1"
                     /translation="QVADAAAEAKQDPYYQAMKNIRLFGQIYQEINDRYIQEIDPEKF
                     IRAGINGMLDRLDPYTVFLEEDGKDELEIITRGKYFGVGMRIQIRNGWATVGEQPFPE
                     SPAARAGIREGDQIIEIDGKSTRGVKLSETAHRLRGTEKGSEVVVKIRRVGEEHPLTF
                     TLLRDEIKVTDIQYSGMVAPGIGLIKLSSFNRSAGDQIRDEVQKLKDQGLEGLILDLR
                     SNPGGLLEAAVTVTENFVARGDMIVFTEGRGEYRRQEHRARKEPVLGSLPLVVLVDGY
                     SASSSEIVAGAIQDLDRGVLIGSPTFGKGLVQTVIPLDRQGDTQLKITTMQYFTPSGR
                     NIQKPEVFNYGPQSVFLNGTSEESGEEEGMAEDPEADEEEESGPAAVDSAPAEKKQEA
                     PAKSYLTRNQRTVLGGGGLKPDVEITPEVMSRYELELLRRSMFFNFSLDYAAAHPDLP
                     GSFEVDDALLEEFNAFCREKKFDYKPEGYTELEKLEKSAREGGWLPQIARHLEAVKTE
                     FEAVKERDRLASKEDLKLALKTEIAGKLFGRDDYYRTLFAADSCVIRGIAVLKNAEEY
                     NRLLGNTKK"
BASE COUNT          371 a          678 c          664 g          498 t
ORIGIN      
        1 ccacatcgga gtacatcatg atgaggcggc gataggcttc gtaggcgaaa cgctcgttgc
       61 cggtcttttt gatcaaaccc ttgatggtct tgctggtgag accgacattg aggacggtct
      121 ccatcatgcc cggcatggag ctgcgcgcgc cggagcggac cgagagcaga aggggatcct
      181 ggacatcgcc gaagcgggcg tccatgatct tttcgaccgc agccagggca gcctcgacat
      241 ccttcaccag ctcgggggga ttctttttgc cgtgggcgta gtagtaagtg cacatctcgg
      301 tggtgatggt aaagccggcg gggacaggga tgctgaggtt ggccatctcc gccaggttgg
      361 cgcccttgcc gccgagcagt tccttcatct tggctgttcc ttcagccttg ccgccgccga
      421 aggaatagac atatttggtt ttggacattc aagcctcctg aaatgccatg gtgattggtt
      481 gggttatttt ttggtgttgc ctaaaagcct gttgtattct tccgcgtttt tcagcaccgc
      541 aatgcccctg atcacgcacg aatcggccgc aaacagggtc cggtaataat cgtcgcgacc
      601 gaagagtttg ccggcgatct cggtcttcaa ggccagcttg agatcctctt tcgacgccag
      661 acggtcccgc tccttcaccg cctcgaactc ggtcttgacg gcctcgagat gtctggcgat
      721 ctggggcagc cagcctccct cccgggcgct tttttcgagc ttttccagct cggtatagcc
      781 ctccggcttg taatcgaatt tcttctcccg gcaaaaagcg ttgaactcct cgagcagggc
      841 gtcatccacc tcgaaactgc ccggcagatc cggatgggcg gcggcatagt cgaggctgaa
      901 gttgaagaac atcgagcgcc gcagcagctc cagttcatac cggctcatca cttcgggagt
      961 gatctcgaca tcgggtttca agccgcctcc cccgagcacg gtgcgctggt tgcgggtcag
     1021 atagcttttg gccggggcct cctgcttttt ctccgccggg gcggaatcga cagctgcggg
     1081 gccgctctcc tcctcttcat cggcttccgg atcctccgcc atcccctctt cttccccgct
     1141 ctcctcgctg gtcccgttca gaaaaaccga ctgcgggcca taattgaaga cctcgggttt
     1201 ctggatgttg cggccgctcg gggtgaaata ctgcatggtc gtaattttca gctgggtgtc
     1261 cccctggcga tcgaggggga tcaccgtctg caccaggcct ttgccgaagg tcgggctgcc
     1321 aataagcacc ccgcggtcga ggtcctggat cgcgccggcg acgatctccg aggaggaggc
     1381 gctgtagccg tcgacaagga cgaccagggg caggctgccc agcaccggct ccttgcgcgc
     1441 gcggtgttcc tggcggcggt attccccgcg cccctcggtg aagacgatca tgtcgccccg
     1501 ggcgacaaag ttctcggtca cggtgacagc cgcctcgagc agtccgcccg gattgctgcg
     1561 caggtcgagg atcagtccct ccagcccctg gtccttgagc ttctgcacct cgtcgcggat
     1621 ctgatcgccc gccgacctgt tgaagctgct cagcttgatc aatccgattc ccggggcaac
     1681 catgccgctg tactgaatgt cggtcacctt gatctcatcg cggaggaggg tgaaagtgag
     1741 gggatgctcc tcgccgacgc ggcggatctt gaccaccact tcgctgccct tttcagtgcc
     1801 gcgcagacgg tgggcggtct cggagagttt cactccgcgg gtgctcttgc cgtcaatctc
     1861 gatgatctgg tccccctcgc ggatgccggc gcgggcggcg ggactctccg ggaagggctg
     1921 ctctccgact gtcgcccaac cgttgcggat ctggatgcgc atcccaacgc cgaaatactt
     1981 gccgcgggtg atgatctcga gctcatcctt gccatcctct tcgagaaaaa cggtataggg
     2041 atcgagacgg tcgagcatgc cgttgatgcc ggcgcggatg aacttttcgg ggtcgatctc
     2101 ctggatatag cggtcgttga tctcctgata gatctggccg aaaagccgga tattcttcat
     2161 cgcctggtaa tagggatcct gcttcgcctc tgcggcggca tcagcgacct g
//
LOCUS       DAOPWU010000489         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_52716_length_2210_cov_4.651972, whole genome shotgun sequence.
ACCESSION   DAOPWU010000489 DAOPWU010000000
VERSION     DAOPWU010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52716_length_2210_cov_4.651972"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PKM23_15500"
     CDS             complement(<1..1178)
                     /locus_tag="PKM23_15500"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNY92928.1"
                     /translation="MIRFLLKGILHDRHRSLMPLIVITIGVALTVLGHAWIQGVMGEA
                     YTMSAHFATGHLKVMTRAYADDQGQMPVDLALTGSSGLTERLAAEMPEIDWAPRIRFG
                     GLIDFPDSAGETRAQGPVAGYAIDLLSPATREPGRFNLPEALLSGRMPRDAREALLTH
                     DFAEKFAVQPGDPFTFFGSTMEGSLAFYNFTVAGTVRFGSEVLDHGAFIIDLTAGQQA
                     LAMQDAAGEILGFFRSGHFDARAAQRLTDRFNSSQPAGTDEFAPVMITMRDQEGMAQL
                     MDYTNAIGSIMVLLFVVAMSVVLWNTGLIGGLRRHGEFGVRLALGEEKGHIYRSLIGE
                     ALLIGLIGSVTGTLLGLGLAWYMQVVGLDIGDAMKASTMMVPAVVRTRISATDFWIGF
                     L"
     gene            complement(1252..>2210)
                     /locus_tag="PKM23_15505"
     CDS             complement(1252..>2210)
                     /locus_tag="PKM23_15505"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNY92929.1"
                     /translation="SLQEAHAPLSPQALELAAAGRIVPELITQATAYPDGRMVNLLIR
                     GIPPGQQLLSLPTAALAADSVGIPALIGRRMAASLRLQPGDALLLRWRDRSGVFDARE
                     VTIVGIFRADVPSIDQGQIWLPLATVQEMTGMAGEATLLVSAPDQAPLPLAGWVFKDT
                     DFLLKEFTEVIHSKKAGSSIIYGTMLAIALLAIFDTQVLSIFRRRREIGTYMALGMTR
                     GQVIGLFTVEGAAHSLFALLLASIYGLPLLHWLGTHGISMPEVADATGMTIGDKMLPV
                     YSAGMVLFSVAVVILSTAIVSYLPARRIARLKPTEALRGRSQ"
BASE COUNT          388 a          665 c          811 g          346 t
ORIGIN      
        1 aggaagccga tccagaaatc ggtggcgctg atccgggtac gcaccaccgc gggcaccatc
       61 atggtggaag ccttcatggc gtcgccgata tcgagtccca ccacctgcat ataccaggcc
      121 aggccaaggc cgagaagcgt gccggtcacc gacccgatca ggccgatgag gagggcctcg
      181 ccgatcaggc tgcggtagat gtggcccttc tcctcgccga gggcgaggcg gacgccgaac
      241 tcgccgtggc ggcgtagacc gccgatgagt ccggtgttcc agagcaccac cgacatggcc
      301 accacgaaga ggaggaccat gatcgagccg atggcgttgg tatagtccat caattgcgcc
      361 atgccctcct ggtcgcgcat ggtgatcatc accggggcga actcgtccgt cccggcgggc
      421 tggctgctgt tgaagcggtc agtgaggcgc tgcgccgccc gggcgtcaaa atggccgctg
      481 cggaaaaagc cgaggatttc cccggcagcg tcctgcatgg ccagggcctg ctgtccggca
      541 gtaagatcga tgatgaaagc gccgtggtcg agcacctccg agccgaagcg gacggtgccg
      601 gcgacggtga aattgtaaaa ggccaggctg ccctccatgg tgctgccgaa aaaggtgaag
      661 gggtcgcccg gctggacggc gaatttctcg gcgaaatcgt gggtgaggag ggcctcgcgg
      721 gcatcgcggg gcatgcggcc cgagagcagg gcctcgggga ggttgaaacg gccgggctca
      781 cgcgtcgccg gcgagagcag atcgatggca tagcccgcca ccggtccctg ggcgcgggtt
      841 tcgccggcgc tgtcggggaa gtcgatgagc ccgccgaaac ggatgcgcgg cgcccagtcg
      901 atttcgggca tctccgcggc cagccgctcg gtcagtccgc tgctgccggt gagggcgagg
      961 tcgaccggca tctggccctg atcatcggcg taggcgcggg tcatcacctt gaggtggccg
     1021 gtggcgaagt gggcgctcat ggtgtaagcc tcgcccatca ccccctgaat ccaggcgtgg
     1081 cccagcacgg tgagggcgac accgatggtg atgacgatga ggggcatcag gctgcgatgg
     1141 cggtcgtgca gaatgccctt gaggaggaat cggatcacgc gggttctcct ttcaaaccct
     1201 gtcgatgggg gagggcaggt ttttcccggc tcgctctttt ccacgccggc ctcattggct
     1261 tctgcctcgc agggcttcgg tgggcttgag ccgggcgatc cggcgcgccg gcagatagct
     1321 gacgatggcc gtggagagga tgacgacggc caccgaaaag agcaccatgc cggcactgta
     1381 gaccggcagc atcttgtcgc cgatggtcat gccggtggcg tcggcgactt cgggcatgga
     1441 gatgccgtgg gtgccgagcc aatgcagcag gggcagcccg tagatcgagg ccagcagcag
     1501 ggcgaagagg ctgtgggccg ccccctcgac ggtgaagagt ccgatgacct ggccgcgggt
     1561 catgcccagg gccatatagg tgccgatctc gcgcctccgc cggaagatcg agagcacctg
     1621 ggtgtcaaag atcgccagca gggcgatggc gagcatagtg ccgtagatga tgctcgaacc
     1681 ggcctttttg ctatggatga cctccgtgaa ctccttgagg aggaaatcgg tatccttgaa
     1741 gacccagccc gcgagcggga gaggggcttg gtccggggcc gagaccagca gggtggcttc
     1801 accggccatg ccggtcatct cctgcacggt tgcgagtggc agccagatct gcccctgatc
     1861 gatggaaggg acgtcagccc ggaagatccc gacgatggtg acctcgcggg cgtcaaagac
     1921 cccgctgcgg tcgcgccagc gcagcagcag ggcgtcgccc ggctgcagcc tcagggaggc
     1981 cgccatacgc cggccgatga gggctggtat ccccaccgag tcggccgcca gtgcggcggt
     2041 ggggaggctg aggagctgct gcccgggggg gatgccgcga atgagcagat tgaccatgcg
     2101 gccgtcggga taggccgtgg cctgggtgat caactccggg acgatgcggc cggcggcggc
     2161 cagctccagg gcctgggggc tgaggggggc atgggcctcc tgcagcgaaa
//
LOCUS       DAOPWU010000490         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_52732_length_2210_cov_2.931323, whole genome shotgun sequence.
ACCESSION   DAOPWU010000490 DAOPWU010000000
VERSION     DAOPWU010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52732_length_2210_cov_2.931323"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKM23_15510"
     CDS             <1..213
                     /locus_tag="PKM23_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92930.1"
                     /translation="QQRTRAQLEELLHAAERERRESETAPWQTQNLLRYALAPGVELV
                     VDARAPAEVRKRVLRLLEQSKIIFST"
     gene            274..1617
                     /locus_tag="PKM23_15515"
     CDS             274..1617
                     /locus_tag="PKM23_15515"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92931.1"
                     /translation="MPDSFVLKERPLGGLFLAEQPEASSLPLDEQRQRHIIFFTDGAV
                     STEEEVLERVRRGIRNFRLFTFGIGPAVNRAFISSLARLGRGTSEFLQLDEDIETAII
                     RFHDRLSYPLLTDLRLRWEGTTAWDMAPVELPDLYYGQPLQLCARFRPGAGARLILSG
                     RCGGADWEKSLPLPAVQARDEMILRTWARARIDALKEELHTESRRASRHRDEVISLAL
                     EYHLVTDFTSLVAVQEEAVSQTPATRLKVAVPLPRGLEKEGFLADSCLNYSMMMPNLQ
                     AAPAIAHQLRSHVSFRLTERSGSQPENVLAGLARRQKAGGSWGDAEAAMEWTCAAVLA
                     FIRSGYTLARGPYRRILEKAVDWLMRQAPVDTLAQQMRALALWEMKQFCSHPLLNGVG
                     AAEEAIVHSIPEPDFRVVRTPAQLRLAAMSRVNVEIRYTPQGGEVGELWLECLKR"
     gene            complement(1760..>2210)
                     /locus_tag="PKM23_15520"
     CDS             complement(1760..>2210)
                     /locus_tag="PKM23_15520"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNY92932.1"
                     /translation="KHTVERYLDLLEKVFVLKKVSGFSRNLHKEVAKTCRYYFWDNGI
                     RNAVINNFSPLDSRSDTGMLWENLLFIERLKRNSYKRHFANYYFWRTYDRQEIDFVEE
                     YNGELWGYEFKWSDRPAKAPKAWSQTYPEAHFETIHRGNFLSFVTQK"
BASE COUNT          423 a          633 c          689 g          465 t
ORIGIN      
        1 cagcagcgca cgcgtgcaca gctggaagag ctgctgcacg ccgcagaaag ggagcgccgc
       61 gagagcgaaa cggcaccttg gcaaacgcag aacctgctgc gttacgccct ggcccccggc
      121 gtcgaactgg tggtggatgc ccgcgcgccg gcggaggtgc gcaagcgggt gctgcgcttg
      181 ctcgaacagt ccaaaatcat tttttcgacg taaggtcact catggtgtcg tgcggattac
      241 acagtggtgg atgatatgga atggaggggt gtcatgcctg attcgtttgt tttgaaagag
      301 cggccgctgg gtggattatt cctggcggag cagcccgagg cctcgagcct gccgctggat
      361 gaacagcgcc agcgccatat catctttttc accgacgggg cggtctccac cgaagaggag
      421 gtactggaga gggtgcggcg ggggatccgc aactttcgcc tcttcacctt cggaatcggg
      481 cccgccgtca atcgcgcctt catcagcagc ctggcgcggt tgggccgcgg caccagcgaa
      541 tttctccagc tcgatgagga tatcgagacc gcgatcatcc gtttccatga ccgtctctcc
      601 taccccctgc tcaccgatct gcgtctgcgc tgggaaggga ccaccgcatg ggatatggcg
      661 ccggtcgaac tgcccgatct ctattatggc cagcctttgc agctctgcgc caggttccgc
      721 cctggagccg gggcccggct gatcctctcc ggccgctgcg gcggcgcgga ttgggagaag
      781 agcctgcctc tgcctgctgt ccaggcccgc gatgaaatga ttctgcgcac ctgggccagg
      841 gcccgcatcg atgccttgaa agaagagctg catactgaat caaggcgtgc ctccaggcac
      901 cgggacgaag tcatcagcct cgccctggaa taccatctgg tcactgattt cacctccctg
      961 gtcgcggtgc aggaggaggc cgtttcccaa acgccggcca cccggctcaa ggtggcggtg
     1021 cccctgccgc gagggctgga gaaggaggga tttttggcgg atagctgttt aaactattcc
     1081 atgatgatgc ccaacttgca ggcagcgccg gcgatcgcgc atcagctccg ctcgcatgtg
     1141 agttttcgac ttaccgagcg gtctggatcg cagccggaga acgtcttggc cgggttggcc
     1201 cgccgccaaa aggccggcgg cagctggggc gacgccgaag cggcgatgga gtggacctgc
     1261 gctgccgtgc tggctttcat ccgcagcggc tacaccctgg cgcgcggacc ctaccgtcgc
     1321 attctcgaga aggcggttga ctggctgatg cgccaggcgc cggtcgacac cctggcacag
     1381 cagatgcgcg ccctggccct gtgggagatg aagcagttct gttctcatcc cttgctgaat
     1441 ggagtgggcg ccgcagagga ggcgatcgta cattccatcc cggagcccga tttcagggtc
     1501 gtgcgtacgc ccgcccagct gcgcctggcg gcgatgagcc gggtcaatgt cgagattcgc
     1561 tataccccgc agggaggtga ggtcggagaa ctctggctgg agtgtctcaa gaggtgatcg
     1621 aattgcggca ggattctttc cagaaatggg ttcgcccagg gaatgaaaac cctgggcgga
     1681 atcaatattt tcaggtttct gcctgaatac ccgtccagga ctgttggcca tcagggaggg
     1741 cggtgtcaat taaaatgcat catttttggg tgacaaagga taaaaaatta ccacgatgga
     1801 tcgtctcaaa atgcgcctcc ggataggtct gcgaccaggc tttgggcgct ttggccggcc
     1861 ggtcgctcca cttgaattca tatccccaga gttcgccatt gtactcttcg acaaaatcga
     1921 tttcctggcg gtcgtaggtg cgccagaaat aatagttggc gaaatgcctc ttgtatgaat
     1981 tgcgtttcag gcgctcgatg aataacaggt tttcccaaag catgccggta tcgctgcggc
     2041 tgtccagggg gctaaagttg ttaatcacag cgttgcgaat cccgttatcc cagaagtaat
     2101 agcggcacgt tttggcgacc tctttgtgga ggttccttga gaagccgctt acttttttca
     2161 gcacgaaaac cttctccaaa agatcgaggt agcgctccac cgtgtgtttg
//
LOCUS       DAOPWU010000491         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_53218_length_2196_cov_5.444652, whole genome shotgun sequence.
ACCESSION   DAOPWU010000491 DAOPWU010000000
VERSION     DAOPWU010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53218_length_2196_cov_5.444652"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PKM23_15525"
     CDS             complement(<1..1064)
                     /locus_tag="PKM23_15525"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNY92933.1"
                     /translation="MKAFRVLILMTAMLIMSLLQSALPQAINLLRNPGFESGLLSGWS
                     AVDSSRVEITARAGHGGKKSLAATPLRAWGGVACDAAALLQPGYRYEISAWLRNAEAG
                     WGQFDLFLVWQEAGKEKSLPIGRVDCDKLTWSSLVQTFTVPEMAAPASLRLVFRNALD
                     HIAFLVDDVVLRPALQLRTTRPAGAAAPAIQAQMGPGTKQRRPVAAALQIFDIRNQTA
                     ARLSRPLDSLITPSLPDGFYRLTAEARDLDGRLFTAEKSLFVGQREDLMLALAGQVDA
                     LLAEPGLSRYHGWISYLNYLIGYARERDGDEGERTLQACYRLSQWLDKVHTDPALLDT
                     LSGVQEWAYLSRVDDTGQPF"
     gene            1508..>2196
                     /locus_tag="PKM23_15530"
     CDS             1508..>2196
                     /locus_tag="PKM23_15530"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HNY92934.1"
                     /translation="MRKLLSILVLISVAGGAAQQIDKKVYTTQFCKLAAPVIDGRGDD
                     AVWQQAEPGEGFTQHEPDNGAPASEASFFKIVYDADNLYVLIRNQDRQPDRISARLTR
                     RDNDEQADGAGIILDSYFDRRTAFTFGVTAAGVKQDFIFSNDGENEDESWDPVWFAQT
                     TIDDSGWTAELRIPFNQLRYPDRPEQVWGLEIYRELARNDETALWQHIPKDASGVVHF
                     FGELHGLKNLA"
BASE COUNT          453 a          666 c          673 g          404 t
ORIGIN      
        1 ttgaaaggct ggccggtgtc atccaccctg gagagatagg cccactcctg cacaccgctc
       61 agggtgtcca gcagggcggg atcggtgtgg actttgtcga gccactggct caggcggtag
      121 caggcctgca gggtgcgttc accctcgtcg ccatcgcgtt cgcgcgcata gccgatcagg
      181 tagttgaggt aactgatcca gccgtggtag cggctcagtc ccggttcggc gaggagggca
      241 tcaacttgtc cggcgagagc cagcatcaaa tcctctcttt ggccgacaaa taggcttttt
      301 tcggcagtga agagacggcc gtccaggtcc cgggcctcgg cggtgagccg gtaaaaaccg
      361 tcgggcaggg agggggttat gagcgagtcg aggggccggc tgaggcgggc ggcggtctga
      421 ttgcggatgt cgaaaatctg cagcgcggcc gcgaccgggc ggcgctgctt ggtgcccggg
      481 cccatctggg cctggattgc cggcgctgca gcgccggcgg ggcgcgttgt gcgcagctgc
      541 agggccgggc gcagcacgac gtcatcgacg agaaaggcga tatggtccag ggcgttgcgg
      601 aagaccaggc gcaggctggc cggcgccgcc atttccggca cggtgaaggt ctggaccagg
      661 ctgctccagg tcaacttgtc gcagtcgacg cggccgatgg gaaggctctt ctcctttccg
      721 gcctcctgcc agaccaggaa gagatcgaac tgtccccacc ccgcctcggc attgcgcagc
      781 caggcgctga tctcatagcg atatcccggc tgcagcagcg cggcggcatc gcaggccacc
      841 ccgccccacg ctcgcagcgg cgtcgccgcc agggatttct ttccgccatg cccggcgcgg
      901 gcagtgatct ccacccggct cgaatccaca gccgaccagc cgctcagcag cccgctttca
      961 aagcccggat tccggaggag attgatcgcc tgcggcaggg cggactgtaa aagagacatg
     1021 atcagcatgg ctgtcatcag aatcaaaaca cgaaaggctt tcattagctc tctccaggtc
     1081 tgaggtgctg agattttcaa ggtgtgagga agcggaacac gaccatacct ctggccggac
     1141 aacggtgacc gacccggcag caaatgcaaa tataattcta tctatgaacg ggatcaagat
     1201 aattatgcca cgcttgtcat ttttctcttt cgcatcgcgg cggacgccgg ataattgccg
     1261 caatcaacga ggccaccgct tccggccggc aaaacccggc aattctccag gctgaccgcc
     1321 ggactggaaa agtgcactgt aacattcttt ccgctttatc gttccataac ttagcccaag
     1381 tgcacatttc tgctgtcgtt tgaaagagaa tgctgatcgg ctgaaatctc ccttcccgcc
     1441 cgctgagccg gagccgatgg cgcgacaccc cgaatagcac ccgacaaaca gatgagaggc
     1501 cgtcgcagtg agaaaactgt tatccattct ggttttgatc agcgtcgcag ggggagcggc
     1561 gcagcagatc gataaaaaag tgtatacgac gcaattctgc aaactggctg ctccggtcat
     1621 cgacggccgg ggcgacgatg ccgtctggca gcaggccgag cccggcgagg gattcaccca
     1681 gcatgagccg gacaatggcg ctcccgccag cgaggcctct ttttttaaaa tagtctacga
     1741 tgcggataac ctctatgtgc tgatccgcaa tcaggaccgt cagccggaca ggatcagcgc
     1801 acggctgacc cgtcgcgata acgacgaaca ggcggatggc gccggcatca tcctcgacag
     1861 ttatttcgac cgccgcaccg ctttcacctt cggagtcacc gctgccggag taaaacagga
     1921 tttcatcttc agcaacgacg gcgagaacga ggatgaaagc tgggatccgg tctggttcgc
     1981 ccaaaccact attgacgact ccggctggac cgccgaactg cgcattccct tcaaccagct
     2041 gcgctacccg gaccggccgg agcaggtctg ggggctggaa atctatcgcg agttggcgag
     2101 gaatgatgaa accgcactct ggcagcatat ccccaaggac gcctccggcg tcgtccactt
     2161 tttcggagag ctgcacgggc tgaagaatct cgccca
//
LOCUS       DAOPWU010000492         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_53789_length_2181_cov_3.820790, whole genome shotgun sequence.
ACCESSION   DAOPWU010000492 DAOPWU010000000
VERSION     DAOPWU010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53789_length_2181_cov_3.820790"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1995)
                     /locus_tag="PKM23_15535"
     CDS             complement(118..1995)
                     /locus_tag="PKM23_15535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92935.1"
                     /translation="MKKALLFLICIFPVTVWGITNMKANGETELTITSVPALLEITAD
                     LSRIGAVLRGGVYADINTNNIFDPVDYSWNWRFGYTIDGVGWIKDPANPLASILGDEG
                     APDGKLRVIFPLLRQQVKLWPRGTIFIFMQDEDGSSGVIRLHLDIKTSAPAIIGKVTS
                     AATGAPIADLALMAMRDSGNQTFDFVNGRTDAEGNYALLADVGNWTVYADGAGEGTGA
                     YMPAHSGPLSLGSSEILVRNIALTPFSALLKGRVVHEDGTPVPQVLLYATGAGYLVAR
                     TDEEGRYIMGTEPGFTQIGLAHNMLANYMHLSQYYEEKPFVNVIATAGENTGLDFVLK
                     KYTSFIRGRCTVEGRGVMGAEVTAVFTDPATGNGRTSSCLSDADGHYLIGVMPGTVAS
                     LNITANGLDAVEPAGMRLNLAAAANDTLKGMDWEFRIRQGRNSISGTVVGPAGTPAEG
                     VCVVAVEANTLYYDSYFFQFTNARGEFNFAGLKNGEWRVGVYKKGASVSPAMIYHSLL
                     GGQNIEGARFTLSGFTGISGKSGAPQGFTLEQNYPNPFNGATSIHFTLAHPGDVSLFI
                     RDLLGREVRRIDRPGLSAGARQIHWDGRDDRGGELPSGIYFYTLEADGETAQARMLMV
                     R"
BASE COUNT          436 a          659 c          644 g          442 t
ORIGIN      
        1 cctccagttt gtatagcgcc attttacctc cttgcggtaa aacatcggtc agcaaaccaa
       61 gaacaacaag gcctctgggc agggctgcag ccgagcagtc cggagggcca gccccggtca
      121 ccggaccatg agcatcctcg cctgagccgt ctctccgtcg gcctccaggg tgtagaaata
      181 gatgccgctg ggcagctccc ctcccctgtc gtcgcggccg tcccagtgga tctgccgggc
      241 gccggcgctc aggccggggc ggtcgattcg ccgcacctcc cggcccagaa ggtcacgaat
      301 gaaaaggctg acatcgcccg gatgggcgag ggtaaaatgt atcgaggtcg cgccgttgaa
      361 ggggttggga tagttctgtt caagagtgaa tccctgcggc gcgccggact tgccggagat
      421 tccggtaaat cccgacaggg tgaagcgcgc cccctcgata ttctggcccc cgagcaggga
      481 atggtaaatc atggccggag agaccgaagc ccccttttta tagacaccca cccgccactc
      541 accgtttttc aatcccgcaa aattgaattc accccgggca ttggtaaatt gaaaaaagta
      601 actgtcgtag tacagggtat tcgcctcaac agcgacgacg cagactccct ccgcgggggt
      661 tccggcgggc ccaaccacag tgccgctgat actgttgcgt ccctgcctga tccggaactc
      721 ccagtccatt cccttcaggg tatcgttggc cgccgccgca agattgagcc gcataccggc
      781 cggctccacc gcatccagcc cattggcggt aatattgaga ctggcaaccg tccccggcat
      841 taccccgatg agataatggc cgtctgcatc gctcaggcag gaagaggtgc ggccgttacc
      901 ggtcgccgga tcggtaaata cggcagtcac ctcggcgccc atcacccccc gtccctcgac
      961 tgtgcagcgc ccgcggatga aggaggtgta tttcttcagg acaaaatcga ggccggtatt
     1021 ctcgccggca gtggcgatga cgttgacgaa gggtttctct tcgtaatact gcgacagatg
     1081 catatagttg gccagcatat tgtgggccag gccgatctgg gtaaacccgg gttcggtgcc
     1141 catgatgtag cggccctctt catcagttct ggcgaccagg tagccggctc cggtcgcgta
     1201 gagaagaacc tgcggcaccg gcgtgccatc ctcgtgtaca accctcccct tgagcagggc
     1261 gctgaagggc gtgagcgcaa tattcctgac caggatttcg ctgctgccaa ggctgagcgg
     1321 gcctgaatgc gcgggcatat aggctcctgt gccctctccg gccccgtcgg catagaccgt
     1381 ccagttgccg acgtcggcta gaagggcata gttaccctcg gcatcggttc ggccgttgac
     1441 gaaatcaaag gtttgatttc cgctatcccg catggccatc agggccaggt ctgcgatcgg
     1501 cgccccggtg gccgccgagg tcaccttgcc gatgatagcg ggcgcactgg tcttgatgtc
     1561 aagatggagc ctgatcaccc cggaggagcc gtcctcgtcc tgcataaaga tgaagatggt
     1621 gcctctgggc cagagcttga cctgttgacg cagcagggga aaaatcaccc tcagcttgcc
     1681 atccggcgcc ccctcatcac cgaggatcga agccagggga ttggcaggat ccttgatcca
     1741 gccgacgccg tcgatcgtat agccaaaccg ccagttccag ctgtaatcga ccggatcaaa
     1801 gatgttgtta gtattgatgt cggcgtagac tccgcccctc agcacggccc cgatgcgcga
     1861 gagatccgcg gtgatctcga gcagggcggg gaccgaggtg atggtcagct cggtctcgcc
     1921 attggccttc atgttggtga tgccccagac tgtgacggga aagatgcaga ttaaaaagag
     1981 cagtgctttt ttcacaacag cctccttcca taatcctgtt cagcatcgtc aaggtgtgta
     2041 aaacatgccg gttccattgt cctgcgtgga agcctgttac cgaggtgcct gctgctgcta
     2101 aaggtgcgcc ggcctcccgg tggtcttcaa ccggagggcc tatttgagca ttttgagcag
     2161 atagatatgt tcgagggcga t
//
LOCUS       DAOPWU010000493         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_54254_length_2168_cov_5.053478, whole genome shotgun sequence.
ACCESSION   DAOPWU010000493 DAOPWU010000000
VERSION     DAOPWU010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54254_length_2168_cov_5.053478"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKM23_15540"
     CDS             complement(<1..203)
                     /locus_tag="PKM23_15540"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HNY92936.1"
                     /translation="MKSRLRTFDLWNMFWRCLFIQGSWNHKSMLGLGFGFSAIPFIKR
                     LFKTQKQREECLRRHLDFFNSHP"
     gene            complement(203..907)
                     /locus_tag="PKM23_15545"
     CDS             complement(203..907)
                     /locus_tag="PKM23_15545"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HNY92937.1"
                     /translation="MWQDVVVVSLWGGFVALDTTAAMQIVISRPLVSCSIVGLLLGSF
                     SPGFIIGLMFELIYLDELPVGGALFNEGNLGATVAAGLAILLGRESGRPDLAVAFAAL
                     AGLLVSKAGAHIVVALRTLNASIYTRLMERPRITPGAVTRAHLLCLLLMFAAGTGLTL
                     AGLVVFFFVFRNLAPLVPAGWDFHLRSLVYVFLGIGCAVLLYLFYERKRWWPLAAGAG
                     LGLLFWLLTALAAGGG"
     gene            1145..>2168
                     /locus_tag="PKM23_15550"
     CDS             1145..>2168
                     /locus_tag="PKM23_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNY92938.1"
                     /translation="MLPIYLDYNATTPIDPEVAAAMLPFMVNAFGNPSSTHWYGLQGR
                     RAVEEARAQVAALLGCHPDEILFTSGGSEANNLALKGYCLAHRERGNHLIVSAVEHPA
                     VMEVARHLVSRGFELTILPVDGYGMVSPADVAAALRPDTLLVSVMHANNEVGTIEPIA
                     DLAAIAHAHGALFHCDAAQSAGKIPVNVDELGVDLLSIAGHKLYAPKGIGCLYVRRGV
                     TLEKQIHGANHERDLRAGTENLLEIAGLGAACRIAARDLETLSARMAAQRDRLWEGLG
                     ARLDGLQRNGHPVQCLPNTLSVSFHKLEANALLARLEEVAASAGAACHSDSITISPVL
                     EEGVGLQ"
BASE COUNT          461 a          764 c          626 g          317 t
ORIGIN      
        1 tagggatggc tgttgaaaaa atcgagatgg cggcgcaggc actcctcgcg ctgcttctgc
       61 gtcttgaaca acctcttgat gaaggggatc gctgaaaagc cgaagccgag ccccagcatc
      121 gatttgtgat tccacgaccc ctggatgaaa agacagcgcc agaacatgtt ccagagatca
      181 aaagtgcgca gccgcgattt catcatccgc ctcccgcggc cagcgccgtc aagagccaga
      241 agaggagccc gagccccgcc ccggcggcca gaggccacca gcgtttacgc tcgtaaaaga
      301 ggtagagcag aaccgcgcag ccgatgccaa gaaagacata gaccaggctg cgcagatgga
      361 aatcccagcc cgccggaacc aggggggcga gattcctgaa aacaaaaaag aacacaacaa
      421 gaccggccag ggtcagcccc gtaccagctg cgaacatcag cagcagacag agcagatggg
      481 cgcgggtgac cgcccccggg gtgatgcgcg gacgctccat cagccgggtg taaatcgaag
      541 cattgagggt gcgcagggcg acgacgatgt gtgcccccgc cttggaaacc agcagccccg
      601 ccagggcggc gaaggccacg gccagatccg gccgcccgct ctcccgcccg agcaggatcg
      661 ccagcccggc ggcgacagta gcgccaaggt tgccctcatt gaagagggcg ccgccgaccg
      721 gcagctcgtc gaggtagatc agctcgaaca tcaggccgat gatgaatccc ggggaaaagc
      781 tgcccagcag cagcccgacg atcgagcagg agaccagggg acgggagatc acgatctgca
      841 tcgccgcagt ggtatcgagg gcgacaaatc cgccccacag gctcaccacg acaacatcct
      901 gccacataag cccactcccg gcaaatgaac gtgttccaga acggggcgca gaatcctgca
      961 ataaaaggaa ttatacacat atttgcccag aaatacaaca aaaaacacag ctccggccca
     1021 gcccgccgca tcccgcgccc gcggcccggc caggctgctt ttaaaaaaca gccgaaacgg
     1081 aaaaccattc cattccaccc cgttttttac tatatttcac cgtactcact gccggagaat
     1141 atatatgctc cccatctatc ttgactataa cgccaccacc cccatcgacc ccgaggtcgc
     1201 cgccgccatg ctgcccttca tggtcaacgc cttcggcaac ccctccagca cccactggta
     1261 tggcctccag ggccggcgcg ccgtggagga ggcccgcgcc caggtcgccg ccctgctcgg
     1321 ctgccacccc gacgagatcc ttttcaccag cggcggctcc gaggccaaca acctcgccct
     1381 caagggttac tgcctggccc atcgcgagcg ggggaatcat ctcatcgtct ccgcggtcga
     1441 gcacccggcg gtgatggagg tcgccaggca tctggtgagc cggggttttg aacttaccat
     1501 cctgccggtc gacggttacg ggatggtctc ccccgccgat gtggcggcgg cgctgcggcc
     1561 ggataccctg ctggtcagcg tcatgcacgc caacaacgag gtcggcacca tcgaacccat
     1621 cgccgatctg gcggccatcg cccatgccca tggtgccctg tttcattgcg acgccgccca
     1681 gtcggccggc aagatcccgg tcaatgtcga tgagctgggg gtcgacctgc tttccatcgc
     1741 cggccacaag ctctacgccc ccaaggggat cggctgcctt tatgtccgcc gcggcgtgac
     1801 cctcgaaaaa cagatccatg gcgccaacca tgaacgcgac ctccgcgccg gcaccgagaa
     1861 cctgctcgag atcgccggac tcggggcagc ctgccgcatc gccgcccgcg acctcgaaac
     1921 cctcagcgcc cgcatggccg ctcagcgcga ccgcctctgg gagggcctcg gcgcccgcct
     1981 cgacggcctg cagcgcaacg gccatcccgt gcagtgcctg cccaataccc tctcggtcag
     2041 ctttcacaag ctggaggcca acgccctcct cgcccggctg gaggaggtcg ccgcctcggc
     2101 cggggccgcc tgccacagcg acagcatcac catctcgccg gtgctcgagg agggcgttgg
     2161 cctccagc
//
LOCUS       DAOPWU010000494         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_55270_length_2142_cov_3.508385, whole genome shotgun sequence.
ACCESSION   DAOPWU010000494 DAOPWU010000000
VERSION     DAOPWU010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55270_length_2142_cov_3.508385"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..817
                     /locus_tag="PKM23_15555"
     CDS             287..817
                     /locus_tag="PKM23_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92939.1"
                     /translation="MIQEIKARNLNTEKIIAEKTAEIQAAVGKGMAINALSGGVDSSV
                     VTMLGHMALGKQLRTVIIDNGIMREGEPEQVAAWFKKLGVKVEIIDARKEFFAALKGL
                     TDPEEKREAITQTFYKKVFGRIVRESRDARTARPTRLPWALLEGLAAEILKKVPGVVS
                     VTYNIATKPPSTIEAV"
     gene            967..1170
                     /locus_tag="PKM23_15560"
     CDS             967..1170
                     /locus_tag="PKM23_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNY92940.1"
                     /translation="MSIAKVTEIISASPKSFQDAIESGIERANKTLENVSGAWISDQK
                     VDIKNGKIVQYRVTMRITFILKD"
     gene            1274..>2142
                     /locus_tag="PKM23_15565"
     CDS             1274..>2142
                     /locus_tag="PKM23_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92941.1"
                     /translation="MRSPVLLMLLMCSALWAQQVQVEVQVPLDSAGVLVEISPELQTR
                     TQLFPEVEDFLSARLFQGGDSSFVLEIAYREEGRLARRRSLMTAAQVRTFRQELTHAL
                     QSPARTQRMDQEGRPGLIVRQTLMGLGLYGWTVPRMMEIDGTRGTIAAYMLTSAASFA
                     LPYYLTRNRPVTTTQTMMTTYGSTRGLLYGLFVRNMVAPDAYSDAAEWGPPLLGSLIG
                     SVAAFRLAEKSGLTLGSAELIGVMGDFGSGFGLGTTHLAELWNGDDRERAAHSLMLGM
                     TGAGLAGGYLLSQR"
BASE COUNT          453 a          628 c          636 g          425 t
ORIGIN      
        1 acatttccgg tgaatcgaag aaatcaccca gcttcatata ggccgcgtgc agctccaggt
       61 ccataaaaac actgggcgta tagcagaggt tgagattggc ctcggcgccg gtaatattgc
      121 cgccctggct gggcgcgaac cggcacaccc gtcggatgga tgcaagaaga tttcagcgtc
      181 attctttttc gaaaactgct tgtatatcgg cctgcatttg gttaattttt ctgatatgta
      241 tgcggcaaag aaactgcaag ttctcgatac cggaaaggag tcaataatga tccaggagat
      301 caaagcccgc aacctcaata ccgagaaaat tatcgcggaa aagacggcgg aaatccaggc
      361 ggccgtcggc aaggggatgg ccatcaacgc cctctccggc ggagtcgatt catcggttgt
      421 gaccatgctc ggccatatgg cgctgggcaa gcagctgcgc acggtcatta tcgataacgg
      481 catcatgcgc gaaggggagc cggaacaggt cgccgcctgg tttaaaaaac tgggcgtcaa
      541 ggtcgagatc atcgacgccc gcaaggagtt ctttgcagcc ctcaaggggc tcaccgatcc
      601 cgaggagaag cgcgaagcca tcacccagac tttttacaaa aaggtcttcg gccgcatcgt
      661 ccgggagagc cgggatgcgc gcaccgcccg gccgacacgg ctgccctggg ccctgctgga
      721 agggctggct gcggagattc tcaaaaaggt gcccggggtt gtcagcgtca cctacaacat
      781 cgccaccaag ccgccctcga ctatcgaagc cgtctgagtg ccaatatcca ccgctgccct
      841 tgcagcggga tgaaaccggc aggaacaatt tgcttgcagt tttgcgttat aatgtacaac
      901 actgggcacg gtctccgcag ggaggccggc cggaattccc tgatgcacta acacaaggag
      961 ctacccatga gcatcgccaa agtgaccgag atcatctccg cttctcccaa aagttttcag
     1021 gatgccatcg aaagcggcat cgagcgggcc aataaaacgc tcgagaacgt cagcggcgcc
     1081 tggatcagcg accagaaggt cgatataaaa aatggcaaga tcgttcagta tcgcgtcacc
     1141 atgcgcatca ccttcatcct caaggattaa atttcaccat ttcccgcgca ggcgcagcct
     1201 ttcaggatgt gcctgcagcg ggctcatgcg ggcggtcgcg gtttggccgc ccttttttag
     1261 aaaggatgct ccaatgcgtt ctccggtctt gttgatgctc ctcatgtgca gtgctttgtg
     1321 ggcgcagcaa gtgcaggtgg aggtccaggt ccctctggat tccgctggtg ttctggtgga
     1381 gatctcgccc gagctgcaga cacgaaccca gctctttccc gaggtggagg attttctctc
     1441 cgcccgcctc ttccagggcg gcgactcctc ctttgttctg gagatcgcct accgcgagga
     1501 ggggcgcctg gccagaagga gaagcctgat gaccgcggcc caagtccgta ctttccggca
     1561 ggaactgaca catgctcttc aatcgccggc ccgcacgcag cgcatggacc aggagggccg
     1621 gcccggcctg atcgtccgcc agacattgat gggcctcggc ctttacggct ggacggtgcc
     1681 ccgcatgatg gagatcgacg gtacgcgcgg aaccatcgcc gcctacatgc tcaccagcgc
     1741 cgccagtttt gccctcccct actacctgac gcgaaaccgc ccggtcacca cgacccagac
     1801 catgatgacc acctatggtt cgacgcgggg tctgctctac ggcctctttg tcaggaacat
     1861 ggtggcaccg gacgcctatt cggatgcggc cgaatggggg ccgccgctgc tggggagcct
     1921 catcggttcg gtggccgctt tccggctggc tgaaaagagc ggcctgacac tgggcagtgc
     1981 ggaactgatc ggagtgatgg gtgatttcgg ctcaggtttc ggactgggca caactcatct
     2041 cgcggagttg tggaacgggg atgaccgcga acgggcggct cattcgctca tgctcgggat
     2101 gacgggagcg ggactggccg gaggctacct gctttcacag cg
//
LOCUS       DAOPWU010000495         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_55537_length_2135_cov_3.196635, whole genome shotgun sequence.
ACCESSION   DAOPWU010000495 DAOPWU010000000
VERSION     DAOPWU010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55537_length_2135_cov_3.196635"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2135
                     /locus_tag="PKM23_15570"
     CDS             <1..>2135
                     /locus_tag="PKM23_15570"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNY92942.1"
                     /translation="LLGAWAAFQEHLARLPENDAATSITREKWLLHLFRELGYGRLAP
                     AKAIELGDKNYPVSHTWQTTPIHLVGWGVDLDKRTAGVAGASRSSPHSMVQELLNRSG
                     DLLWALLANGRRLRILRDNVSLTRQAWVEFDLEAMMQGEVYADFVLLWLLCHQSRMEA
                     DRPENCMLERWSRAAQEQGARALDQLRSGVESAITALGRGFLRHPDNKPLRQRLYAGE
                     LDKQDYYRQLLRLVYRLIFLFVAEERRLLLLPDAPAESKRRYNDYYSTRRLRSIAARR
                     RGSKHADLYQGLCVVLQKLGNEGGCPELALPALGTFLFSGQAAPDLDAAQIANHDLLE
                     AIRHLAVIIDGRSRRQVDYKNLGSEELGSVYESLLELHPRLELASGDFSLHTAGGHER
                     KSTGSYYTPSSLIQELLDSALDPVLDAAARQPDPEAAILSLKICDPACGSGHFLFAAA
                     HRIAHHLAAAVTGESEPAPEALRAALRRVIGHCIYGVDINEMAVELCKVGLWLEALEP
                     GKPLSFLDHHIQCGNSLLGVTPELMAAGIPDEAFSPIEGDDKTVCSAARRLNKSERET
                     GQADLFYAASAPWQRLGDFAGAMAAMDRVSDETLAGYRSKSSRYQEIVNSPAFLFGRL
                     LADLWCAAFVWKKTRNFPYVVTNEVFRTVEREPGNIPTWMYEEVERLRRQYGFFHWHL
                     AFPEVFAAAPSTSGEPADRSGAGPTAGGF"
BASE COUNT          366 a          749 c          638 g          382 t
ORIGIN      
        1 ctcctcggcg cctgggccgc ctttcaggag catctcgcca gactgccgga aaacgacgct
       61 gccacctcca tcacccgcga gaaatggctg ctccacctct tccgagagct gggctatggc
      121 cgtctcgccc ccgctaaagc gatcgaactc ggtgacaaaa actatcccgt ctcgcacacc
      181 tggcagacca cgcccatcca tctggtgggc tggggtgttg atctggacaa gcgcaccgcc
      241 ggggtggccg gggccagccg cagctcgccc cacagcatgg tgcaggagct ccttaaccgc
      301 tccggggatc tgctctgggc gctgctcgcc aacggccgca gactgcgcat cctccgcgac
      361 aacgtcagcc tcacccgcca ggcctgggtt gaattcgacc tcgaggcgat gatgcagggc
      421 gaggtctatg ccgatttcgt cctgctctgg ctgctctgcc atcaatcgcg gatggaggcc
      481 gaccgccctg aaaactgcat gctcgagcgc tggtcccggg cggcgcagga gcagggcgcc
      541 cgcgccctcg atcagctgcg cagcggcgtg gaatcggcca tcaccgcctt gggccggggc
      601 tttctccgcc atcccgacaa caaacccctg cgccagcgtc tctatgcagg cgagctggac
      661 aaacaggact attaccggca gcttttacgc ctggtctatc gcctcatctt tcttttcgtt
      721 gccgaagagc gccggcttct gctgctgccg gatgcaccag cggagagcaa gcggcgctat
      781 aatgactatt attcgacccg gcgcctgcgc agcatagccg cccgccgccg ggggagcaag
      841 cacgccgatc tctatcaggg cctgtgcgtc gtgctgcaaa aattggggaa tgagggcggc
      901 tgtcccgagc tggccctgcc ggccctgggc acttttctct tttccggcca ggccgcaccc
      961 gatctcgatg ccgcccagat cgccaaccac gatctgctcg aggccatccg ccacctcgcg
     1021 gtcatcatcg acggccgctc acggcggcag gtggattaca aaaatctcgg ctcggaggaa
     1081 ttgggcagcg tctatgaatc cctgctcgag ctccaccccc gcctcgaact ggcgagcgga
     1141 gatttcagtc tccatactgc cggcggacat gaacgcaaat cgaccggcag ctattatacc
     1201 ccctccagcc tcattcagga gctgctcgat tcagccctgg atccggtgct cgatgccgcg
     1261 gcccggcagc ccgatccgga ggcagccatc ctcagcctga agatctgcga tcccgcctgc
     1321 ggcagcggcc atttcctctt tgccgcggcg caccgcatcg cccaccacct cgccgccgcc
     1381 gtcaccggcg agagcgagcc cgcccccgag gccctgcgcg ccgccctgcg ccgggtcatc
     1441 ggccactgca tttacggtgt ggacatcaac gagatggcgg tggaactgtg caaggtcggc
     1501 ctctggctgg aggcgctcga gccgggcaaa cccctctcct ttttggatca tcacatccag
     1561 tgcggcaact cgctgctggg cgtgacgccg gagctgatgg ccgccggcat ccccgatgag
     1621 gctttttccc caatcgaggg agacgacaaa acggtctgca gcgcggccag acggctcaac
     1681 aagagcgagc gcgagaccgg ccaggcggat ctcttttatg cggcctcggc gccctggcag
     1741 cggctgggcg acttcgccgg ggccatggct gccatggacc gcgtcagcga cgagactctg
     1801 gccggctacc gcagcaaatc gagccgttac caggagatcg tcaactcgcc cgcctttctt
     1861 ttcggccgcc tgctggccga tctctggtgc gccgcctttg tctggaaaaa gacgcgcaat
     1921 ttcccttatg tcgtcaccaa cgaagttttt cgcaccgtcg agcgcgaacc gggaaacatc
     1981 cccacctgga tgtacgagga ggtggagcgg ctgcggcggc agtacggatt ttttcactgg
     2041 catctggcat tccccgaagt ctttgccgct gcaccctcaa catccggcga gccggcggat
     2101 cgcagcggag ccggccccac cgcgggcggc tttga
//
LOCUS       DAOPWU010000496         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_55574_length_2134_cov_3.614719, whole genome shotgun sequence.
ACCESSION   DAOPWU010000496 DAOPWU010000000
VERSION     DAOPWU010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55574_length_2134_cov_3.614719"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKM23_15575"
     CDS             <1..189
                     /locus_tag="PKM23_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92943.1"
                     /translation="LERQLLLNKNSELRKQAAKQLRGKYPVFYNEAELRRILLSLGSG
                     GDAVAHQKEEAEGPDDVE"
     gene            298..1275
                     /gene="dusB"
                     /locus_tag="PKM23_15580"
     CDS             298..1275
                     /gene="dusB"
                     /locus_tag="PKM23_15580"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNY92944.1"
                     /translation="MILAGIEIKEPVVLAPLAGWTDTVYRQLCKEFGVGLIFTEMASA
                     DALVRNQTRTLDICRFTEAERPIGVQLFGAEADTLARAAEIVAELRPDCIDINFGCPA
                     KKVVKRGGGSALMRDLSLIEEIGRAVVAATPIPVTAKLRSGWSDTVVVEACLQLEQAG
                     VAAVTIHPRTQKMGFKGRADWRLIAEVKRALKIPVIGNGDIAAPADARRMFEETGCDL
                     VMIGRAAQGNPWIFSRTIAQLRTGVDVPPPTNAERLALCIRHLDLQARHYGEHVAVYT
                     MRKHIASYLKGVPNVAEFRDQLFRKNDIGEVRADLLAFADNLEETTVNA"
     gene            1401..>2134
                     /locus_tag="PKM23_15585"
     CDS             1401..>2134
                     /locus_tag="PKM23_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HNY92945.1"
                     /translation="MSKRKAIIMGAAGRDFHNFNLHFRQNSDYEVVAFTATQIPNIDD
                     RKYPAELAGALYPKGIPIEPEDSLPELIRRHNVDEVFFAYSDVPHDYVMHRASLVNGF
                     GASFSLLGPRFTMIKSRVPVVAVCAVRTGCGKSQTTRRVAEILMGKGKRVVAIRHPMP
                     YGDLVKQRVQRFETLEDLKRHECTIEEMEEYEPHISRGIVVYAGVDYGAILEQAEKEA
                     DVILWDGGNNDTPFYVPDLHIVVVDPL"
BASE COUNT          435 a          627 c          647 g          425 t
ORIGIN      
        1 ctggaacgtc agctcttgct caataaaaac agcgagctgc gcaaacaggc ggccaagcag
       61 ttgcgcggca aataccccgt tttctacaac gaggcagagc tgcgccgcat cctcctttcg
      121 ctgggttccg ggggagatgc ggtcgcacac cagaaggaag aggctgaggg tcctgatgac
      181 gttgagtgac cggccgcagc gggagttgct ggaacaagcg tcccgccacc tgcgtggtgg
      241 ggggacggcc gggcaggatt cccttgctta ttacttttta atgagttata ttagcctgtg
      301 atccttgctg gcatcgagat taaggaacca gtcgtcctgg cgcccctggc gggctggacc
      361 gacacggtct atcggcaatt atgcaaagag ttcggcgttg gtctcatctt caccgaaatg
      421 gccagcgccg atgccctggt gcgcaaccag acgcgcaccc tcgatatctg ccgtttcacc
      481 gaagcggagc gccctatcgg tgttcagctt ttcggggctg aggccgatac ccttgcgagg
      541 gcggcggaaa tcgtcgccga gctgcggccc gattgtatcg atatcaattt cggctgtccg
      601 gccaaaaagg tggtcaaacg gggaggcggt tcggccctga tgcgggacct gtcgcttatc
      661 gaggagatcg ggcgagcggt agtcgcggcc acccccattc cggtgaccgc caaactgcgc
      721 agcggctgga gcgacacggt ggtggtcgag gcctgccttc agctcgaaca ggcgggtgta
      781 gccgccgtca ccatccaccc gcgcacccag aagatgggct tcaagggcag ggccgattgg
      841 cggctgattg ccgaggtcaa gcgagccctt aaaattccgg tcatcggcaa cggggatatc
      901 gccgctccag cggatgcccg gcgcatgttc gaagagaccg gctgcgatct ggtgatgatc
      961 ggtcgcgcgg cccagggcaa tccctggatt ttttcccgga cgatcgccca gctccgcacc
     1021 ggtgtagatg tcccgccccc gacaaatgcc gaacggctgg ccctctgcat ccgccatctc
     1081 gatctgcagg cccggcacta tggagaacat gtggccgtct atacgatgcg caagcatatc
     1141 gccagttatc tgaagggagt gcccaacgtc gccgaattca gggatcagct cttccgtaaa
     1201 aacgatatcg gtgaggtcag agcggatctg ctcgcctttg ccgacaacct ggaggagacg
     1261 acggtcaacg catagcagca tctttgaggc aggagcaccg catcctgcct tttttacatt
     1321 ttaaagatca ccggccggag agggagggca ctgacctgcc gcatccggct caacttactg
     1381 tacttctctc cggaggatgc atgtcgaagc gcaaagcgat tatcatgggt gcagcaggca
     1441 gggatttcca caatttcaat cttcacttcc gtcagaacag cgactatgag gtggtggctt
     1501 ttacagcgac gcagattccc aacattgatg accgcaagta tccggcagaa ttggctggag
     1561 cactctatcc caagggcatc cccatcgagc ccgaggatag cctgcccgag ctcattcgcc
     1621 ggcacaatgt cgatgaggtt tttttcgcct acagcgatgt tccccatgac tatgtgatgc
     1681 atcgggcctc actggtcaat ggtttcggag ccagcttttc gctgctcggc cccaggttta
     1741 cgatgatcaa atccagggtg ccggtggtgg cggtttgcgc cgtgcgcacc ggctgcggca
     1801 agagccagac cacgcgccgg gtcgccgaga ttctgatggg caagggcaaa agggtggtcg
     1861 ccatccgcca tccgatgccc tacggggatc tggtcaagca gagggtgcag cgtttcgaga
     1921 ccctcgagga tctcaaacgg cacgagtgca ccatcgagga gatggaggag tacgaacccc
     1981 atatttcccg cggcatcgtc gtctacgccg gcgtcgatta cggcgccatc ctcgagcagg
     2041 ccgaaaaaga ggcggatgtc atcctctggg acggcggcaa caacgataca cccttttacg
     2101 tgcccgacct gcacatcgtc gtggtcgatc ccct
//
LOCUS       DAOPWU010000497         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_56210_length_2117_cov_3.647915, whole genome shotgun sequence.
ACCESSION   DAOPWU010000497 DAOPWU010000000
VERSION     DAOPWU010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56210_length_2117_cov_3.647915"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PKM23_15590"
     CDS             complement(<1..760)
                     /locus_tag="PKM23_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY92946.1"
                     /translation="MSIPGLSIIAESINDSVPSTHALFEENNIAGIVELARLQAEKGA
                     AYIDVNVGRRSPGFMAEVVHRIQEHITLPLSIDTPDPELAAAGLYAYNPERAGNRKPI
                     LNSISGARLEMFDLFARQPFMPILLVTEGLDDSGEMVMNKSAAQNHATALEMVQIAEL
                     AGRMPNELSGGQQQRVALARAFVIEPSVLLMDEPLSNLDAKLRVQMRATIKKLQRKLG
                     ITTIYVTHDQEEAMAISDRIAVMKNGLIMQVGEPA"
     gene            complement(816..1577)
                     /locus_tag="PKM23_15595"
     CDS             complement(816..1577)
                     /locus_tag="PKM23_15595"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HNY92947.1"
                     /translation="MRLRLLGCEVLTREICHSLASSPHVIDAEFTKINMHDTPEELHR
                     LIQCRIDAAAAGGYDAILLCYGLCGNATAGLCARHIPLIIPRAHDCCTLLLGSKSAFN
                     SRFADQPSSRFTSVGYMERSAESVASHPGTIARAIGLPERYDDYVYKYGEENARYIWE
                     TLVEAAETSAGDNRLYFIDIPEASPPGGAARCEELARTAGLQCVRLQGSIALVEKLVM
                     GEWGSEDFLTVAPGQCIAGVYDFDEIIAARDLAAE"
BASE COUNT          434 a          593 c          624 g          466 t
ORIGIN      
        1 gcgccggctc gcccacctgc atgatgagcc cgtttttcat caccgcgatg cggtcggaga
       61 tggccatggc ctcctcctgg tcgtgggtga cataaatggt ggtgatgccc agctttcgct
      121 gcaatttctt gatggtggcg cgcatctgca cgcgcagctt ggcgtcgagg tttgaaagcg
      181 gctcgtccat cagcagcacg ctgggctcga tcacaaaggc gcgggccagg gccacgcgct
      241 gctgctggcc gccggaaagc tcgttgggca tgcgcccggc aagctccgcg atctgcacca
      301 tctccagcgc cgtggcgtga ttctgggctg cactcttgtt cataaccatt tccccggaat
      361 catccagccc ctcggtgacg agcagaatcg gcatgaacgg ctggcgggcg aagaggtcga
      421 acatctccag gcgcgccccg gagatcgagt tcagaatcgg cttgcggttg ccggcccgtt
      481 cgggattgta ggcatacaag ccggcggcag cgagctcggg atcgggggta tcgatggaaa
      541 gaggcagggt gatgtgctcc tggatccggt ggacgacctc cgccatgaat ccgggagaac
      601 ggcgtccgac attgacatcg atataggcag cgcctttttc agcctgcagc ctcgccagct
      661 cgacaattcc cgcgatatta ttctcctcaa agagggcatg ggtggagggc accgaatcgt
      721 tgatcgactc agcgatgatg ctcaaaccgg gtatactcat aattatcatt cctttaaatg
      781 gtttattgcc tgctcgcaac gcccctgccg caggattatt ccgcagccag atcccgggcg
      841 gcgatgatct cgtcgaaatc gtagacgccg gcgatgcact ggcccggagc aacggtcaga
      901 aaatcctcac tcccccactc tcccattacc agtttttcga ccagggcgat gctgccctgg
      961 agccgcacgc actgcaaacc tgccgtcctg gccagctctt cgcagcgtgc ggcgccaccg
     1021 ggagggctgg cctcggggat gtcgatgaaa tagaggcggt tgtcgccggc cgaggtttct
     1081 gccgcctcca cgagcgtctc ccagatgtag cgggcgttct cctcgccgta tttgtaaaca
     1141 tagtcatcgt acctctccgg caggccgatg gcgcgggcaa tggtgccggg atggctggcg
     1201 acggactctg cactcctctc catgtatcca acggaggtga agcgtgaact tggctggtcg
     1261 gcgaagcggg aattgaaagc cgatttcgag ccaaggagca gggtgcagca gtcgtgggcc
     1321 cgcgggatga tcagggggat atgccgggcg cacagccctg ctgtggcatt gccacagagc
     1381 ccgtagcaga ggagaatcgc gtcataaccg ccggctgcgg ccgcatcgat gcggcactgg
     1441 atcagccggt ggagttcctc cggggtgtca tgcatgttga ttttagtgaa ctcggcatcg
     1501 atcacatgcg ggctggacgc cagagagtga cagatttcac gggtcaggac ctcacagccc
     1561 agaagcctca gccgcaccgg aaattcctca attttatctg acagaaactg aagacggcgc
     1621 agctgctgcc gcctcccccc tctcgatctg atggaaaatt gaataaaaat agcagaaatc
     1681 atcctcattc gcaagcaaaa gttggccggt ggattttggc cggatttcgc gttagcaatt
     1741 agtggcttaa aaatacaaca ttaatagtat ttagtggtat tttttctaaa tccataagga
     1801 ttattttaac tggcctgtat ggttatagat gttgtcgaca tattgtagtc agacattttt
     1861 tcccggggca tcttggcagg ggtctttctt cagcactctt ctcttcctgg tacatctctg
     1921 gcgcagcatt aaaattcgga cgcagcactg aatgcagcag gcgtctgtat tgtggcgtta
     1981 ttaaccggca tcattgggac gcatcagcca atcgggaaga ccgtatgaga tcactattct
     2041 caaccacttt tgcgattctc ctggtattgg ggggaatggc aggcctgcag ggccagacca
     2101 cggtggatcc cgccagc
//
LOCUS       DAOPWU010000498         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_56624_length_2106_cov_4.496343, whole genome shotgun sequence.
ACCESSION   DAOPWU010000498 DAOPWU010000000
VERSION     DAOPWU010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56624_length_2106_cov_4.496343"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PKM23_15600"
     CDS             complement(<1..694)
                     /locus_tag="PKM23_15600"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY92948.1"
                     /translation="MKKTTCIIPLLILLILAGSGFGQSFAPVGTSVAQFLEVGTGARA
                     TGLGQAFTGMATGAEAAFWNPAGMADISGHNFYSSYSTWPADISLMGLSYGLNLGSIG
                     IIGISGVYLMTDDMEITTLNQPGGTGEYFNIANFSMGLSYARYLTDRFSVGVTGKVVH
                     EKYLTNGYTTWALDLGTMYHTDFHGLRLGMSILHFGPEVSFSGSYIDFSDSKSYSAKK
                     SKEFNSYSLPINF"
     gene            complement(751..>2106)
                     /locus_tag="PKM23_15605"
     CDS             complement(751..>2106)
                     /locus_tag="PKM23_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92949.1"
                     /translation="WGAESRFCNIDDYVLSNAQPWNSWIKDFPQMAGRAHSLTEADLD
                     SSMIFMWDGDNPSYPADNGVDDDFGNPGADGRLQTPGFLGFKILKTEPYLKPSSFHQC
                     QIYNDPASDLEAWTKMISVHTYDGLIYSKGKPYAYDFRGILSFGPIEKFMYGDSFKVT
                     AALSVGSDPDSGGVYSLMKLVKNFNTAQFIVDNDYKVSSEALAPAAPQVRLEQVLDGS
                     QVTGIRVVWDDAATAHAYFEGYKIWKAVGKNSQGAYDWVPLGAGTYSTLAGGTWPPPA
                     GDQAGTFALVDNDIVNGFEYYYAVQSYTRDINDPIPLGVIQSNILNSIKSIAPANPVA
                     ATLDNVKVVPNPYIGSVIWNNPMPSDNEPWQHRIQFTNLPADATVKIFTLDGDYIDEI
                     RAGYTARRTADNVAIEGADSVAEWDIITRNNQEAAPGIYMFVVDSPSLGQKIGKFVII
                     Q"
BASE COUNT          409 a          547 c          696 g          454 t
ORIGIN      
        1 ggaaattgat cggcagcgag tagctgttga actccttgct ctttttggcg ctgtaggatt
       61 tggagtcact gaaatcgata tagctgccgc tgaaactcac ctccgggcca aagtggagga
      121 tggacatgcc caggcgcagg ccgtggaaat cggtgtggta catggtgccg agatccaggg
      181 cccaggtggt atagccgttg gtgagatatt tttcatggac caccttgccg gtgacgccga
      241 ccgaaaaacg gtcggtgaga taacgggcgt aggagaggcc catgctgaaa ttggcgatgt
      301 tgaaatattc acccgtgccg ccgggctggt tgagggtggt gatctccata tcatcggtca
      361 tgagatagac gccgctgatg ccgatgatgc caatgctgcc gaggttgagg ccgtaggaga
      421 gccccatcaa agagatatcg gcgggccatg tgctgtagga gctgtagaaa ttatgaccgc
      481 tgatatccgc catgccggcg gggttccaga aggcggcctc cgcgccggtg gccatgcccg
      541 taaaggcctg gccgaggccg gtcgcccggg cgcccgttcc cacttcgagg aactgtgcca
      601 ccgaggtccc gaccggcgca aagctctgcc cgaagccgga tccggccagg atcaacaaga
      661 tcaggagggg tatgatacag gtcgtttttt tcatgtttca tcctcattga taaggatcaa
      721 aagttccttc cggttgatgc gtgccggccg ttactggatt atcacgaatt tgccgatctt
      781 ctggccgagg gagggcgagt cgacgacaaa catgtagatg ccgggggctg cctcctggtt
      841 gttgcgcgtg atgatatccc attccgccac gctgtcggcc ccctcgatcg ccacattgtc
      901 ggcggtccgg cgcgccgtat acccggcccg gatctcgtcg atataatcgc cgtccagggt
      961 gaagatcttg acggtggcgt cggccggcag gttggtgaac tggatgcgat gctgccaggg
     1021 ctcgttgtcg ctcggcatgg gattgttcca gataacgctg ccgatatagg gattgggcac
     1081 gaccttgacg ttgtccagcg tcgcagccac cggattggcc ggtgcgatgc ttttgatgct
     1141 gttgaggata ttgctctgga tcaccccgag ggggatgggg tcgttgatat cgcgcgtata
     1201 ggactgcacc gcgtaatagt attcaaagcc gttgacgata tcgttgtcga cgagggcgaa
     1261 ggtgccggcc tgatcgccgg cggggggagg ccaggttccg ccggcaaggg tggagtaggt
     1321 tccggcgcca aggggcaccc agtcgtaagc gccctgggaa ttcttgccca ccgccttcca
     1381 gattttatag ccctcgaagt aagcgtgagc ggttgcagca tcatcccaga cgaccctgat
     1441 gccggtgacc tggctgccgt cgagcacctg ctcaaggcgg acctggggag cggccggggc
     1501 cagggcttcg gaggagacct tgtagtcatt gtcgacgatg aactgggcgg tattgaaatt
     1561 cttcaccagt ttcatcaggc tgtaaacccc gccgctgtcc ggatcgctgc ccacagaaag
     1621 ggcggccgtg accttgaagg agtcgccgta catgaatttt tcaatcggcc cgaaggagag
     1681 gatgccgcgg aaatcatagg catagggttt gcccttgctg tagataaggc cgtcataggt
     1741 gtggacggag atcatcttgg tccaggcttc cagatccgag gcgggatcgt tgtagatctg
     1801 gcactgatgg aatgagctgg gcttgagata gggctcggtc ttgaggatct tgaagccgag
     1861 gaagccgggg gtttgcagcc ggccatcggc gccggggttg ccgaaatcgt cgtcgacgcc
     1921 gttgtcggcc ggatagctgg gattatcgcc atcccacatg aagatcatgc tgctgtcgag
     1981 gtcggcctcg gtgaggctat gggcccgtcc ggccatttgg ggaaagtcct tgatccagga
     2041 attccagggc tgggcgttgc tgaggacgta atcgtcgata ttgcaaaagc gcgattcggc
     2101 gcccca
//
LOCUS       DAOPWU010000499         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_58538_length_2060_cov_3.080798, whole genome shotgun sequence.
ACCESSION   DAOPWU010000499 DAOPWU010000000
VERSION     DAOPWU010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58538_length_2060_cov_3.080798"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="PKM23_15610"
     CDS             complement(<1..1825)
                     /locus_tag="PKM23_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY92950.1"
                     /translation="MPPRLKPHRKLDAKTIGREILPNIWRSIRLVWQGSRLLFILTAI
                     LLVIQAVLPLGVLYSSKLILDAVAAALQAGAVAATPPSIILLASLVAGLVFLGVFAGS
                     IHTWLQGHQTALVQEHIMERLHQKSAEIDYAYYENPAFFDTLHRAQHQSIYRPSQLVG
                     RLQTILQQGLTLAGLVGLLFFFHWSIPLLLLLTLLPAFFFRLHFADVLFEWRKKNAAL
                     ERKSYHYHWLLTDKESAKELRVNAAGRLFRRRYLALQRRLRVEQFRINGRQTRGTLTA
                     QLLATVVAYGFYLVLIGQTLAGIITLGSLVMYFQAFQQAINALRGLFTGLGDIYEDSR
                     FVAQLFEFFALQPAVPRVVQPQRVPAPVRAGLSVEGISFRYPGSERWALRDVTFRIAP
                     GEHFALAGANGSGKSTLMKLICRLYDPVEGRICLDGIDIREFELTEWWRNLAVMFQDF
                     EQYNFSARENIWLGDLKMPRRDARVEEAARRSGAAEVIGALPHGYDAMLGKWFDEGEE
                     LSKGEWQRIAVARAFLRDAPIMIFDEPTSALDARNEYEMLNAWLERARGRIALLISHR
                     VSTLQSADRIIMFKEGRIVESGSHQELWRAGGPYAELFNQPI"
     gene            complement(1854..>2060)
                     /locus_tag="PKM23_15615"
     CDS             complement(1854..>2060)
                     /locus_tag="PKM23_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92951.1"
                     /translation="VARTKMLVGLFGGVSYSPHRMVTLLAEYDAECFNAGFRFHPFPL
                     LQAQVAWWKMRELTATLALHVDLM"
BASE COUNT          432 a          632 c          668 g          328 t
ORIGIN      
        1 ggatgggctg gttgaacaat tcggcatagg ggccgccggc gcgccacaac tcctggtggc
       61 tgccggactc gacgatgcgg ccctccttga acataataat acgatcggcg ctctgcagcg
      121 tcgaaacccg gtgactgatc aacaaggcaa tccggccgcg cgcccgctcc agccaggcgt
      181 tgagcatctc gtactcgttg cgggcatcga gggcgctggt gggctcgtcg aagatcatga
      241 tcggggcatc gcgcagaaac gcccgcgcca ccgcgatccg ctgccactct cccttgctaa
      301 gctcctcccc ctcatcgaac catttgccca gcatcgcatc gtatccgtgc ggcagtgcgc
      361 caatcacttc cgcggcgccc gagcgtcgcg ccgcctcctc gacgcgcgca tcccggcgcg
      421 gcatttttaa atcccccagc cagatattct cccgcgcgct gaaattgtac tgctcgaaat
      481 cctgaaacat cactgccaga ttgcgccacc actccgtcaa ttcaaattcg cggatatcga
      541 tgccatccag gcagatccgt ccctcaacgg gatcatagag ccggcagatc agcttcatca
      601 gcgtgctctt gcccgagccg ttggcgcccg ccagggcaaa gtgttcgccc ggtgcgatgc
      661 ggaaggtgac gtcccgcagg gcccagcgct cgctgccggg gtagcggaac gatatcccct
      721 cgaccgacaa acctgcgcgc accggcgcgg gcacacgctg cggctgcacc acccgcggca
      781 cggcgggttg gagagcaaaa aactcgaaga gctgggcgac gaaacgcgag tcctcataga
      841 tatcgcccag gccggtaaag agcccacgca gggcgttgat ggcctgctgg aaagcctgga
      901 agtacatcac caggctgccg agcgtgatga tacccgccag ggtctggccg atcagcacca
      961 gataaaaacc ataggccacc acggtggcga gcagctgggc ggtcagcgtg ccgcgggtct
     1021 ggcggccgtt gatacgaaac tgctccaccc gcagccggcg ctgcagggcc agatagcggc
     1081 ggcggaaaag gcggccggct gcattcacgc gcagctcctt ggccgactct ttgtcggtca
     1141 gcagccagtg gtagtggtag ctcttgcgct cgagcgccgc gttctttttg cgccattcga
     1201 acagaacgtc ggcaaaatgg agacggaaga aaaaggccgg cagcagggtc agcagcagga
     1261 gcagggggat cgaccagtgg aagaaaaaga gcagaccgac cagaccggcc agggtcaatc
     1321 cctgctggag gatggtctgc agacggccga ccagctgcga gggacggtag atggactggt
     1381 gctgggcgcg gtggagggtg tcgaagaaag cggggttctc gtaataggcg taatcgatct
     1441 ccgccgattt ttgatgcagt cgctccatga tgtgctcctg caccagagcg gtctgatgac
     1501 cctgcagcca ggtgtggatg ctgccggcga aaacgcccaa aaagaccagg ccggcgacca
     1561 gcgaagccag caggatgatc gaaggcggcg tcgcggcaac tgcgcccgct tgcagcgccg
     1621 ccgcgaccgc gtccaggatc agcttgctgg aatagagcac gccgaggggc agcaccgcct
     1681 ggatgaccag caggatagcg gtcaggatga agagcagccg cgaaccctgc cagaccagcc
     1741 ggatcgaacg ccagatattg ggcaggatct cccggccgat ggttttggcg tcgagtttgc
     1801 ggtgcggttt caggcgcggg ggcatgcggt cagttcatca gcggcaagcg aggtcacatg
     1861 aggtcgacat gaagggccag agtggcggtg agttcccgca ttttccacca ggccacctgg
     1921 gcctgcagca gcgggaaagg atggaaacga aaaccggcgt tgaagcactc ggcgtcatac
     1981 tcggcgagca gggtcaccat gcggtgggga gaataagaga ctcccccgaa aaggccgacc
     2041 agcattttgg tgcgggcgac
//
LOCUS       DAOPWU010000500         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_58931_length_2051_cov_2.781563, whole genome shotgun sequence.
ACCESSION   DAOPWU010000500 DAOPWU010000000
VERSION     DAOPWU010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58931_length_2051_cov_2.781563"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..989)
                     /locus_tag="PKM23_15620"
     CDS             complement(57..989)
                     /locus_tag="PKM23_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HNY92952.1"
                     /translation="MAISSPDTLHFWIQRFALSLEVERNCSLHTRQGYLRDLGQLEAY
                     LAERFRRETPSVEDLSRSSVRGFLSHLLRQGYAPRSIARKQATLRSFARFLVRESALT
                     VNPLLAIHSPRLPKRLPDFLTREEVQQLLRLPEDKGFAGLRDRAILQLFYATGVRISE
                     AAGLKTGDLQLWEGTLRVHGKRDKTRLLPLGRHMTQTLQEYLRLREPVAALNPNSADF
                     LLLTDEGKSYTRQQLACLIRGYMLRIADKRKAHPHALRHTFATHLLDEGADILSVKEL
                     LGHASLSTTQIYTHLSAEHLRRVYRQAHPRAEEE"
     gene            complement(1103..1570)
                     /locus_tag="PKM23_15625"
     CDS             complement(1103..1570)
                     /locus_tag="PKM23_15625"
                     /inference="COORDINATES: protein motif:HMM:NF016264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HNY92953.1"
                     /translation="MNKRLLEIIAYAMKEIRDNSIENIDLQFIMDVLLERGFSEDEIS
                     SAMAWLVHHGETIDRLIKKQNDGVPRPVWRQLNETERQSISPEAYSYLFHLREMELLN
                     DDDMETIIERAVNIKLPSLDIEDMQDLIAIVVLDFENSASEGYFQYTANHLPN"
     gene            complement(1705..1794)
                     /locus_tag="PKM23_15630"
                     /pseudo
     CDS             complement(1705..1794)
                     /locus_tag="PKM23_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
     gene            1888..>2051
                     /locus_tag="PKM23_15635"
     CDS             1888..>2051
                     /locus_tag="PKM23_15635"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNY92954.1"
                     /translation="MGGAVVFRLHAAQLALLNLAQAGDEVVSTDSLYGGTYTLFHHTF
                     PRLGVRVRFVP"
BASE COUNT          399 a          620 c          613 g          419 t
ORIGIN      
        1 catgcacctc cttctggctg tgcgcgcgcc gggttgtgac tccggcgtgc gctgtactac
       61 tcctcctccg cgcgcggatg ggcctgccgg tagacgcggc gaagatgctc cgcggaaaga
      121 tgggtataaa tctgggtggt tgaaaggctg gcatgcccga gcagttcctt caccgagagg
      181 atgtccgcac cctcgtccag gagatgagtg gcgaaggtgt ggcggagggc atgcggatgg
      241 gccttgcgct tgtcagcgat gcgcagcata tagccgcgga tgagacacgc cagctgctgc
      301 cgggtatagg atttgccctc gtcggtgagc agaagaaaat cggctgaatt cggattgaga
      361 gcggcgacgg gctcgcgcag ccggagatac tcttgcaggg tttgcgtcat gtggcgtccc
      421 aggggcagca gacgcgtctt gtcgcgtttg ccatgcacgc gcagcgtgcc ttcccagagc
      481 tgcaggtcgc cggttttaag ccctgccgcc tcactgattc tgacgccggt tgcataaaag
      541 agctgcagga tcgcgcggtc gcgcagcccg gcaaatccct tgtcctccgg cagacgcagg
      601 agctgctgaa cctcctcgcg ggtgaggaaa tcgggcagcc gtttgggcag ccggggggaa
      661 tggatggcga ggagggggtt gacggtcagt gccgactccc gcaccagaaa acgggcgaag
      721 gagcgcaggg tggcctgctt gcgcgcgatc gagcggggcg catagccctg tcttaaaaga
      781 tgggagagga agcctcgcac cgaggagcgg ctcagatcct caacagacgg ggtctcacgg
      841 cgaaaacgtt cggccagata ggcctccagc tggccgagat cccgcaaata gccttgacgc
      901 gtatggaggg aacaatttcg ttcgacttcc agggagagag caaagcgttg gatccagaaa
      961 tgaagggtat caggtgatga gatcgccatg tagagcccag atttacccgt ctgctcttca
     1021 gcgcgggagc cctctcccgc cgccgcgagg ccaccaaggc gccacctttc ctgaccggct
     1081 gcggtcagat caatcctgtt gctcagttgg ggaggtggtt ggccgtgtat tggaaataac
     1141 cctcggatgc actgttctcg aaatcgagaa ccacgattgc gatcaaatcc tgcatatcct
     1201 cgatgtcaag ggaaggcaat ttgatattca ctgcgcgctc aatgatcgtc tccatgtcat
     1261 catcattgag cagctccatc tcgcgcagat gaaaaagata gctgtaggcc tccggcgata
     1321 tcgactgacg ctccgtctca ttcagctgcc gccagaccgg ccgcggcacc ccgtcattct
     1381 gcttcttgat gagacggtca atggtttcac cgtgatggac cagccaggcc atcgcgctgg
     1441 agatctcgtc ctcggaaaaa ccgcgctcga gcaaaacatc cattataaac tgcaaatcaa
     1501 tattttcaat tgaattatcc ctgatctcct tcatggcata tgcgataatc tcaagcagtc
     1561 ttttattcat ccagttctcc attccaccga tcggggttaa ccggaacggt ctcccaattc
     1621 aatataagaa atatatttgc aaaagcaagc gctttcggag cccgcccccg catttctgcc
     1681 gcacgatccc cgatggagct ccgctcagtt ccggccgcag cagttcttgt acttcttgcc
     1741 gcttccgcag gggcaggggt cattccgccc gacgtggacc gacttgacca ccggcgccgc
     1801 cttggccggg gcctgtgccg gcgccgccgg cccctccccc tgctggcgcg gcgccgcagc
     1861 cccctgctta aagctctcca cctgggaatg ggtggcgctg tagtttttag gctccatgcg
     1921 gcgcagctgg cgctcctcaa cctcgcccag gccggcgacg aggtcgtctc caccgacagc
     1981 ctctacgggg ggacatacac cctcttccac catacctttc cccgtctggg cgtccgggtg
     2041 cgcttcgtcc c
//
LOCUS       DAOPWU010000501         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_59595_length_2036_cov_4.485613, whole genome shotgun sequence.
ACCESSION   DAOPWU010000501 DAOPWU010000000
VERSION     DAOPWU010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59595_length_2036_cov_4.485613"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="PKM23_15640"
     CDS             complement(<1..1331)
                     /locus_tag="PKM23_15640"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNY92955.1"
                     /translation="MKELDIREIIASRAPGLLGKYPPVVERLVISCLGRLLRVEEINS
                     QIRRHGESSGLAFVDEVFEELNFSYYLSSIDRQKIPSEGKLIVVANHPLGALDGLALL
                     RAICDVRADTKIITNEVLMVFENLADFFLPYNVFSAQTQKEHVARIGESLLAEEAVLF
                     FPAAEVSRLSWKGIRDPRWLNGPVYFAKKYDVPVLPVYVRGRNSLLFYIISLLNKRFS
                     TLLLPREIFNKRGRSLRFVVGDPIPGAVFSSSMIKGRVLTRLLRKQTYLLAHKKRSII
                     FKTEKTIIHPVDRRQLKRELASSTLLGRTSDGKKIYLVDFNNAHRVMREIARLREQTF
                     RKVGEGTGLKLDLDRFDRHYQHIVLWDDDALEIIGSYRVGNCRQVIDGHGLDGLYTNT
                     LFKYAAAAAPILDQALELGRSFVQPKYWKSNALDYLWQGIGAYVVNHPEVN"
     gene            1664..>2036
                     /locus_tag="PKM23_15645"
     CDS             1664..>2036
                     /locus_tag="PKM23_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNY92956.1"
                     /translation="MKKIALIALAALMAFSAAVSAQDFIQIKGSDTSVNLVQRLAEVF
                     METNPNTAIAVTGGGSGVGIAALIANKVQVADASRPMKEKEYSAARENGVIATEIAIG
                     IDALSVVVHHDNPIKSLSMAQV"
BASE COUNT          466 a          571 c          579 g          420 t
ORIGIN      
        1 taattaacct ccgggtggtt gacgacatag gcgccgatgc cctgccagag atagtccagg
       61 gcgttgctct tccagtattt gggctgcaca aaactgcggc caagttcgag ggcttgatcg
      121 aggatcggcg cggcggccgc ggcgtatttg aaaagggtat tggtgtagag cccgtcgagc
      181 ccgtgcccgt cgatgacctg gcggcaattg cccacccggt aggagccgat gatttcgagc
      241 gcatcgtcat cccagaggac gatatgctga taatgacggt caaaccggtc gaggtccagc
      301 ttcaatcccg tcccctcgcc taccttgcgg aaggtctgct cccgcaggcg ggcgatctcc
      361 cgcatcaccc gatgggcgtt gttaaaatcg accagataga tttttttgcc gtcgctggtc
      421 ctgccgagca gagtggagga ggccagctcc cgcttgagct gcctgcggtc gaccgggtga
      481 atgatggtct tttcagtctt gaagatgatg gagcgtttct tgtgcgcgag cagataggtc
      541 tgcttgcgta aaagacgggt caacaccctg cccttgatca tgctggagct gaagaccgcg
      601 cccgggatcg gatccccgac aacgaaacgc agcgatcggc cgcgtttgtt aaagatctcc
      661 cgggggagca gaagggtgga aaatcgcttg ttgagcagcg agatgatata gaacaggaga
      721 gaatttctgc cccggacgta aaccgggagc accggtacgt catacttttt ggcaaagtag
      781 accgggccat tgagccatct gggatcgcgg atgcccttcc aggacagccg gctgacctcg
      841 gcagcaggaa aaaacagcac cgcctcctcc gcgagcaggg actccccgat ccgcgccaca
      901 tgctccttct gggtttgcgc ggagaagaca ttgtagggca aaaagaaatc agcgaggttt
      961 tcaaagacca tcaacacttc gttggtgatg attttcgtgt ccgcccgcac atcacagatc
     1021 gcacgcagca gggcaaggcc atccagcgcc cccagaggat ggttggcgac cacaatcagc
     1081 ttgccctccg aggggatttt ttggcgatcg atgctggaaa gatagtagga aaagttgagt
     1141 tcctcaaaaa cctcgtcgac aaaagccaga ccgctggatt cgccgtgcct gcggatttgg
     1201 gagttgatct cctccacccg cagcagccgg ccgagacagc tgatgacgag acgttcaacg
     1261 acagggggat atttccccag taaaccgggg gccctggagg ctatgatctc acggatgtcc
     1321 agctccttca tcacaccatc ctcttgttaa ctcagctgga agaacgatcc cccatgattg
     1381 atcctgccgg gcagatgcac cacaaggaaa gagataacag ggcggcagag gtctgcagca
     1441 tgacaggtcg ctgattttga taatctaaca atattttcca tcggagtcaa aagattaatt
     1501 gctgatgccg atggcccggc gcaaagcctg tgcatttgcg tccacttttt ttcttaaccg
     1561 tttcttaaca cgggcttcat gctcaggaaa catccgtttg ctatcttata ccgtcaaact
     1621 aaagctgcac tgcggggaag aacccaattc tccaggagga gagatgaaaa aaatcgccct
     1681 gatcgccctg gccgccctga tggccttctc ggcagcggtc tccgcccagg atttcatcca
     1741 gatcaaggga tcggatacct cggtaaatct ggttcagcgc cttgctgagg tttttatgga
     1801 aaccaacccc aacaccgcca tcgctgtgac cggcggcgga tcgggtgtcg gcatcgccgc
     1861 cctgatcgcc aacaaggtgc aggtcgcgga tgcctcgcgg ccgatgaagg agaaggaata
     1921 ctccgccgcc agggaaaacg gtgtcatcgc caccgaaatc gccatcggca tcgacgccct
     1981 ctcggtcgtc gttcaccacg acaatccgat aaaatcgctg agcatggccc aggtgg
//
LOCUS       DAOPWU010000502         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_59598_length_2036_cov_3.956083, whole genome shotgun sequence.
ACCESSION   DAOPWU010000502 DAOPWU010000000
VERSION     DAOPWU010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59598_length_2036_cov_3.956083"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKM23_15650"
     CDS             complement(<1..469)
                     /locus_tag="PKM23_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNY92957.1"
                     /translation="MARNIEIKARIGGIAEMEARAAALADRGPIIIEQDDTFFACPNG
                     RLKLRARTVDDGELIFYRRADQSGPKESFYLLARTREPDALRKVLSLAYGTAGRVRKL
                     RTLYWCGRTRIHLDRVEGLGDFLELEVTLAEDEPPAAGEAVARELMEKLGIRAE"
     gene            complement(472..1848)
                     /locus_tag="PKM23_15655"
     CDS             complement(472..1848)
                     /locus_tag="PKM23_15655"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNY92958.1"
                     /translation="MKQSPFSFSAMFQAAADFGSVLVALYLSYWLWELSPFRLNLHII
                     WPTPLQALLVSLFFIFLFFIQGGYERKSSILNVRARKNLISAMLLGCALVIVYSFFTR
                     KIALGRLQIFYLFILMLLLITIQRHLFDKLHTLLLRRGIGAKRVLIFGAGDTGERIAR
                     ALQRYPKMGYLPVGFIDDYKKQGEGEARALPILGGLAELRTIAAESDAREIIIAIPSA
                     TQARMQEVMKGCAAVRLPYRYVPNLYDTAIQRIRTEVIDGVPLFDLAHLHYSPLSALV
                     KRLLDVVLSVLVILISVPLVLLIGLAIRLETPGPVFFRHLRTGRGGKSFMLWKFRTMY
                     SDTDPYAIHPHSRRDPRITRVGWLLRRTSLDELPQFWNVLRGEMSVVGPRPEMPFIAG
                     QYTELQRERLNVRPGITGLWQISADRAVPIHENIDHDLFYIQNQSLLLDLIIIAKTFW
                     VAIAGLGR"
     gene            complement(1859..2029)
                     /locus_tag="PKM23_15660"
     CDS             complement(1859..2029)
                     /locus_tag="PKM23_15660"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNY92959.1"
                     /translation="MKHAPGWMKYMALEWFYRLLQEPNRLWRRYVKNNTLFILYTLRE
                     LLTGTLPGGRSA"
BASE COUNT          407 a          596 c          681 g          352 t
ORIGIN      
        1 cctccgcacg gatgccgagc ttttccatca gttcccgcgc caccgcctcg cccgctgcag
       61 gaggttcatc ctccgcgagt gtgacctcca gttcgagaaa atcccccaat ccctccaccc
      121 ggtcgagatg gatccgcgtc cggccgcacc agtagagggt gcgcagcttg cgcacacggc
      181 cggccgtgcc ataggccagg gagagcacct tgcgcagggc gtcgggctcc cgggttcggg
      241 cgagaagata aaaggactcc ttggggccgg actgatcggc gcggcggtaa aagatcagct
      301 cgccgtcgtc gaccgtgcgc gccctcagct tgaggcggcc gttcggacaa gcgaaaaagg
      361 tatcatcctg ctcgataatg atcgggcccc ggtccgccag ggccgccgcc cgggcttcca
      421 tctccgcgat gccgccgatg cgggccttga tttcaatgtt gcgcgccatc tttacctccc
      481 caaaccggcg atcgccaccc agaaggtctt ggcgatgatg atcagatcca gcaacagcga
      541 ctggttctga atataaaaaa ggtcgtggtc gatgttctcg tggatgggca cggcgcggtc
      601 ggccgagatc tgccagagtc cggtgatccc cggccgcacg ttgaggcgct cgcgctgcag
      661 ctcggtgtac tgtccggcga tgaagggcat ctccggacgc ggcccgacca cactcatctc
      721 cccgcgcagc acattccaga actgcggcag ctcgtcgaga ctggtgcgcc gcagcagcca
      781 gcccacccgg gtgatgcgcg gatcgcggcg gctgtgggga tggatcgcgt agggatcggt
      841 atcgctgtac atggtcctga atttccacaa catgaaggat ttgccgcccc tgccggtgcg
      901 cagatgcctg aaaaaaaccg gacccggagt ctcgagacgg atggccaggc cgatcaacag
      961 caccaggggc acactgatga ggatgaccag cacggagagc accacgtcga ggagccgctt
     1021 gaccagggcc gagagcggcg aatagtggag gtgggcgaga tcgaaaagcg gcaccccgtc
     1081 gatcacctct gtgcgaatgc gctggatggc ggtgtcgtag aggttgggca catagcggta
     1141 gggcaagcgg acggcggcgc agcctttcat cacctcctgc atgcgggctt gcgtcgccga
     1201 ggggatggcg atgatgatct cccgggcgtc gctctcggcc gcaatcgtcc gcagctctgc
     1261 tagtccgccc agaatcggca gggcgcgggc ctctccctcc ccctgcttct tgtagtcgtc
     1321 gatgaatccg accggcaggt agcccatctt gggatagcgc tgcaacgccc tggcgatgcg
     1381 ctcgccggtg tcgcccgcgc cgaagatcag cacccgcttt gcgccgatgc cgcggcgcag
     1441 cagcagggtg tggagcttgt cgaagagatg acgctggatg gtgatgagca ggagcatcag
     1501 aatgaagaga tagaaaattt gcaggcggcc gagggcgatc ttgcgggtga aaaaggagta
     1561 gacgatgacc agagcgcagc cgagcagcat ggcgctgatg aggttcttgc gggcgcggac
     1621 attgaggatg ctgctcttgc gctcatagcc gccctggatg aaaaagagaa agatgaagaa
     1681 gaggctgacc aggagggcct gcaggggtgt cggccagatg atatggaggt tgaggcggaa
     1741 gggcgacagc tcccacagcc agtagctgag atagagggcg accaggaccg agccaaagtc
     1801 ggcggcggcc tggaacattg cgctgaacga gaaagggctc tgcttcatgc tattccgttt
     1861 atgcggagcg tccgcccggg agggttccgg tcagcaattc gcgcagggta tagagaataa
     1921 aaagggtatt gtttttcaca taacgccgcc aaaggcggtt gggttcctgg aggagccggt
     1981 agaaccactc cagggccatg tacttcatcc agcccggagc gtgtttgatg gtgccg
//
LOCUS       DAOPWU010000503         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_59739_length_2033_cov_3.775025, whole genome shotgun sequence.
ACCESSION   DAOPWU010000503 DAOPWU010000000
VERSION     DAOPWU010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59739_length_2033_cov_3.775025"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKM23_15665"
     CDS             <1..324
                     /locus_tag="PKM23_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNY92960.1"
                     /translation="PGDYLWKISGKDEIYDDPMQWMRIYSYNKEQIKDPDLIYPKQEF
                     KIQRECGPDEYLVVKGDNLKKIAANPAVYGDPSAWTKIYQKNKAVIGGDSHRIFPHTV
                     LIIPR"
     gene            542..1507
                     /locus_tag="PKM23_15670"
     CDS             542..1507
                     /locus_tag="PKM23_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNY92961.1"
                     /translation="MNEPVPVEKRIGLRGIDPVDFLGAQDANLRLLEGELDAQVIVRG
                     HEIILRGLPEQVEQAEQIISELVYLANRNGRVIPQDIETVLRMNEKGGTMAEAAPSAE
                     SVLVYTKGGVIKPRTPGQEEYVASAQKNDIVFAIGPAGTGKTFLAVAMAVAALRDHLV
                     DRLILCRPAVEAGESLGFLPGDFREKVDPYLRPLYDALHDMIPGDKLRRLIETRVVEI
                     VPLAYMRGRTLNNCFVILYEAQNTTAAQMKMFLTRLGVNSRAIITGDITQIDLPDSAV
                     SGLVQIQSILKGIDGIGFCYLTDRDVVRHQLVRQIIKAYETLKQT"
     gene            1532..>2033
                     /locus_tag="PKM23_15675"
     CDS             1532..>2033
                     /locus_tag="PKM23_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92962.1"
                     /translation="MNQRFDKNSGKNSLLNGSKGKERRANGGREWLKAFRRHRYLLLG
                     GIILAAFIAILFPGEKSYQFSSLREGRVYIGPEIIASFTFPVNKSHEEYQADLRKARA
                     EVAPVFDRVDSVAEAQLEHLELFVQRAGMVLRSGVMIADEVSRVFQEGGIILSEEDIV
                     NLMSGSA"
BASE COUNT          424 a          585 c          624 g          400 t
ORIGIN      
        1 cccggtgact atctctggaa gatttccggc aaggatgaga tctatgatga tccgatgcag
       61 tggatgcgca tctactccta taacaaggag cagatcaagg atccggatct gatctacccg
      121 aagcaggagt tcaagatcca gcgcgaatgc ggcccggatg agtacctggt ggtcaagggc
      181 gacaatctga aaaagatcgc cgccaatccc gctgtgtacg gcgatccttc cgcctggacc
      241 aagatctatc aaaagaacaa ggcggttatc ggcggagact cccatcgcat tttcccgcac
      301 accgtcttga tcattcccag atagggagag tgtttcaggc tgcgtgcaga gtggcggttc
      361 gggtcgatgc agcagatgct gatctatccc gcctgcatcc ccatttccga gcctatacgg
      421 cagggatgaa ggatggagga ggcgccacgc gcatgtgaaa ttatggactc tcggtcttgc
      481 cacctttggg gcgagaccgg gagtttttta ttgtgaagca aacatcatta cggacaaacc
      541 attgaacgaa cctgtccctg ttgaaaaacg gatcggcctg cgcggcatcg atccggtcga
      601 ttttctcggc gcccaggatg ccaacctgcg actcctggag ggggagctcg acgcccaggt
      661 gatcgtgcgc ggccatgaga tcatcctgcg ggggctgccc gagcaggtgg agcaggccga
      721 acagatcatc agcgagctgg tttatctggc caaccgcaac ggccgggtca ttccgcagga
      781 cattgagacg gtgctgcgca tgaacgaaaa aggggggacg atggcggaag cagcccccag
      841 tgctgagtcg gtcctggtct ataccaaggg gggcgtgatc aaaccgcgca ccccgggtca
      901 ggaggagtat gtcgccagcg cccagaagaa cgacatcgtc ttcgccatcg gtccggcggg
      961 gacgggcaaa acctttctgg cggtggccat ggcggtggcg gccctgcgcg atcaccttgt
     1021 cgaccgcctc atcctctgcc gtccggcggt ggaggcgggc gagagcctcg gctttctgcc
     1081 gggcgatttt cgtgaaaagg tcgatcccta tctgcggccc ctctatgatg ccctgcacga
     1141 tatgatcccg ggcgacaaac tgcgccgtct catcgagacc cgcgtggtgg agatcgtccc
     1201 gcttgcctat atgcgcggac gcactctcaa caactgcttc gtcatcctct atgaagcgca
     1261 gaacaccact gcggcgcaga tgaagatgtt tctcacccgg ctcggggtca actcgcgcgc
     1321 catcatcacg ggcgacatca cccagatcga tctgcccgac agtgcagtat cgggtctggt
     1381 gcagatccag tccattctca aggggatcga cggcatcggc ttctgctatc tcaccgaccg
     1441 ggatgtggtc cgccatcagc tggtgcggca gatcatcaaa gcctatgaga cgctgaaaca
     1501 gacatgacat gggccactac ctggagtgaa gatgaatcag cgctttgaca agaattcggg
     1561 caagaattcc ctgctcaacg gcagcaaagg caaggagcgg cgggccaacg gggggcgcga
     1621 atggctgaag gcgttccgcc ggcatcgcta tctgctcctc ggcggcatca tcctggccgc
     1681 cttcatcgcc atcctcttcc caggggaaaa gtcctatcag ttctcgagtc tgcgcgaggg
     1741 tcgggtctac atcggcccgg agatcatcgc ctctttcaca tttccggtca acaagagcca
     1801 cgaggagtat caggcggacc tcaggaaggc ccgggctgag gtggcgccgg tatttgaccg
     1861 cgtggattcg gtcgcggagg cgcagctcga acacctggaa ctctttgtgc agcgtgcggg
     1921 catggtcctc cgcagcggcg tgatgattgc cgatgaagtg agccgggtct tccaggaggg
     1981 tgggatcatc ctctccgagg aggatatcgt caatctcatg tccggttccg cgg
//
LOCUS       DAOPWU010000504         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_60242_length_2022_cov_3.915608, whole genome shotgun sequence.
ACCESSION   DAOPWU010000504 DAOPWU010000000
VERSION     DAOPWU010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60242_length_2022_cov_3.915608"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKM23_15680"
     CDS             complement(<1..451)
                     /locus_tag="PKM23_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNY92963.1"
                     /translation="MALKKQQKEPSGRMPGSSNSSSSQTPQRPQMFPGRVWLFFLIIL
                     AMNYLLGRVFAPDAEAPVKVPYTLFKQEVAADNVQAVFNKGETITGKFKRPVTWPEAD
                     STEAAALKVEPRKVTAFSTTLPAFIDPGLEAYLISHKVEITAEPIQEK"
     gene            828..1943
                     /locus_tag="PKM23_15685"
     CDS             828..1943
                     /locus_tag="PKM23_15685"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNY92964.1"
                     /translation="MSKPARLISLDAFRGFTIMLMILVNNPGNWDYVFSPLEHAQWHG
                     CTPTDLVFPFFLFIMGSAMAFSFARRLAEEGSRRALYLQILKRTFLLIFLGLFMAWYL
                     RWNLGSLRFPGVLQRIGLCYFFASMIILHTGRRGQIIWTAVLLIGYWLAMALIPFPGR
                     TADVWALNSNLAQYIDGILLKGHLYKQDIGFDPEGLLSTIPAIAQVMLGYFTGQWLRG
                     KAEPLQKTTTLFIAGNIALVLGLAWSLFMPINKQLWTSSYVLYTSGIALNFLGVIYWL
                     IDIKGYTKFTKPFVIFGSNAILAFFGSGIMAKTMYMWKVTRADGTVTSLKGLFCEKLL
                     VPLFGQWGGSLAHALFFILVWLGILTWFYKKKIFIKL"
BASE COUNT          382 a          622 c          542 g          476 t
ORIGIN      
        1 gtttttcctg aatgggctct gcggtgattt cgactttgtg gctgatcaga taggcctcca
       61 ggccgggatc gatgaaggcc ggcagggtgg tcgagaaggc ggtcaccttc ctgggttcca
      121 cttttagggc ggcagcctcc gtcgaatccg cctcgggcca ggtcaccggc cttttgaatt
      181 ttccggtgat ggtctcgccc ttgttgaaga ccgcctggac gttatcggct gccacctcct
      241 gcttgaataa ggtatagggg accttgaccg gcgcttcggc atcgggggcg aagacacggc
      301 cgaggaggta attcatcgcc aggatgatca ggaaaaaaag ccagacccgg cccgggaaca
      361 tttgcggccg ctgcggggtt tgtgaggagg aggaattgga ggagcccggc atccggccgg
      421 aaggctcctt ctgctgcttc ttcagtgcca tatcgggctc cctgataagg gtataggtac
      481 acaactattt taatcactgc tgcagcctct gccggggatg ccggcagaag tcgcgtcccg
      541 acagattgat cgatgaaaat atacataata ttgttcgaaa tgcataccat tttccaacgc
      601 atggagcaac aaactgttcc gcattgcgta agcagtagag gcgcgtccag aggcgccgtc
      661 gcgggtttgc tggaaagagt acggaggggt tggtggcaag cggcgccagg tgtcgggtgg
      721 gagccagatt tagggggtac ccaggcccag aaatcccttg acattctgtc gcaataatct
      781 taaactgaat aaatccgctt tttccctatc ctaacaagag gcagaaaatg tcgaaaccag
      841 cgcgtctcat ctccctcgat gctttccgcg gcttcaccat catgctgatg atcctggtaa
      901 ataatcccgg caactgggat tatgtctttt caccgctcga acacgcccaa tggcacggct
      961 gcaccccgac ggatctggtc tttcccttct tcctcttcat catgggcagc gccatggcct
     1021 tttccttcgc gcggcgtctg gcggaggagg gctcgcgccg tgcgctctat ctgcagattc
     1081 tcaagcgcac ctttctgctc atcttcctcg gcctcttcat ggcctggtat ctgcgctgga
     1141 accttggcag cctgcgcttc cccggcgtac tgcagcgcat cggcctctgc tactttttcg
     1201 cttcgatgat catcctccac accgggcgcc gcggccagat catctggacc gccgtgctcc
     1261 tcatcggtta ctggctggcc atggccctga ttcccttccc cggccgcacc gcggatgtct
     1321 gggccctcaa cagcaacctc gcccagtata tcgacggcat tctcctcaag ggccacctgt
     1381 acaaacagga tatcggcttc gaccccgagg gactgctcag cactattccg gctatcgccc
     1441 aggtgatgct cggctacttc accggacagt ggctgcgggg caaggctgaa cccctgcaaa
     1501 agaccaccac cctctttatc gccggcaaca tcgccctggt gctcgggctc gcctggtccc
     1561 ttttcatgcc gatcaacaag cagctctgga ccagcagcta cgtcctctac acctccggca
     1621 ttgccctcaa ctttctcggg gtgatctact ggctcatcga catcaagggc tataccaaat
     1681 tcaccaaacc ttttgtcatt ttcggcagca atgccatcct cgcctttttc ggctccggga
     1741 tcatggccaa gaccatgtat atgtggaagg tgaccagggc ggacggcacc gtcacttcac
     1801 tcaaggggct cttttgtgaa aaactgctcg tccccctctt tggccagtgg gggggatcgc
     1861 tcgcccatgc ccttttcttc atcctggtct ggcttgggat cctgacctgg ttttacaaga
     1921 aaaagatctt tatcaagctg tagagaccgc cgccccggcc tgaggtcgtc cttttccgtc
     1981 tgaccgggcg cgcgccaatc ctgcacctct ccgcctcccc tc
//
LOCUS       DAOPWU010000505         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_60717_length_2012_cov_4.782831, whole genome shotgun sequence.
ACCESSION   DAOPWU010000505 DAOPWU010000000
VERSION     DAOPWU010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60717_length_2012_cov_4.782831"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKM23_15690"
     CDS             complement(<1..755)
                     /locus_tag="PKM23_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNY92965.1"
                     /translation="MESTTAKLLIVDDEKRMCAILREALVTPRLAVTTAESGEAAWAA
                     LTLERFDVIISDIKMAGISGVELLKRVKASWPETEVLLMTAYADARTAVEAMKSGAFD
                     YIIKPFEIDELRLKVRNILEKRQLRIENLDLRVQLKERFSLENMVGQSGAMQKVYELV
                     EKVAPTDATVIIRGESGTGKELVARAIHQRSRRADQPFIAVNCSALPETLLESELFGH
                     EKGAFTGAERQKPGRFELAGIGTIFLDEIGELTP"
     gene            complement(755..>2012)
                     /locus_tag="PKM23_15695"
     CDS             complement(755..>2012)
                     /locus_tag="PKM23_15695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY92966.1"
                     /translation="LFYNRASYFFLKSIGRQLEESLDQRLAATAALTAELVELGLHSF
                     TDAGEAELLRGTLNRIRLDHELEAAYLVGEDGGILLDARPELEKSVGRPYLMEDSLAI
                     RAAFAGRVQASRLHTVAGNHFKSVYAPVSDVYGRGAVLALEANAGFLQTMDRFYRALY
                     IGVIISVLLLAALALFLILAMLQFLRTETRLYQSERLASLGQMAATVAHEIRNPLAII
                     KSTADVLREKQTQPEKSAEFFDYIDEEIARLNRIVGDFLAYSREPRLEMARHDLVPLL
                     NELAAGFSRQGRRVDCQCTEQQLFFRCDADQIGQMLLNLLLNAQQAMENSGEGDIILR
                     LRKQSILGRSQVVIEVQDHGPGLQGRGREIFEPFFTTRSSGTGLGLAVCKRIVEGHGG
                     EIMALEPGEGGTLMRIIFPGHGGQGE"
BASE COUNT          357 a          640 c          620 g          395 t
ORIGIN      
        1 ggggtcagtt ccccgatctc gtcgaggaat atggttccaa tcccggccag ctcgaaacgt
       61 cccggtttct gccgttccgc gccggtgaaa gccccctttt catggccgaa aagctcgctc
      121 tcgagcaggg tctcgggcag ggcgctgcag ttcacggcga tgaagggctg atcggcgcgg
      181 cggctgcgct gatgaatcgc ccgcgccacc agttccttcc cggtgccgct ctccccgcgg
      241 atgatcaccg tcgcatcggt cggagccacc ttttcaacca gctcgtagac tttttgcatt
      301 gctccgctct ggccgaccat gttctcgagg gagaaacgct ccttgagctg cacgcgcagg
      361 tcgaggttct cgatgcggag ctggcgtttt tcaagaatgt tgcgcacctt gaggcggagc
      421 tcgtcgatct cgaagggctt gatgatatag tcaaaggccc cgctcttcat cgcctccacg
      481 gcggtgcggg catcggcata ggcggtcatg agcagcacct cggtctccgg ccagctggcc
      541 ttgaccctct tgagcagctc cactccgctt atgcctgcca tcttgatatc gctgatgatg
      601 acgtcgaagc gctctagggt gagggcggcc caggcggctt ctccgctctc ggcggtggtc
      661 accgccagcc ggggcgtgac cagggcctcg cgcaggatgg cgcacatgcg cttttcatca
      721 tcgacgatca acagtttcgc agttgtgctc tccatcactc accttgcccc ccgtgtccgg
      781 ggaaaatgat cctcatcagg gtgccgccct cgccgggttc aagggccatg atctcgccgc
      841 cgtgtccctc gacgatcctc ttgcacaccg ccagtcccag gccggtgccg ctgctcctgg
      901 tggtaaaaaa gggctcgaat atttcccgtc cgcggccctg caaaccgggt ccgtgatcct
      961 gcacctcgat gaccacctgg ctcctgccca gaatactctg cttgcgcagg cgaagaatga
     1021 tgtcgccctc cccgctgttc tccatcgcct gctgggcgtt gagtagcaga ttgaggagca
     1081 tctgtccgat ctgatccgca tcgcagcgga aaaagagctg ctgttcagta cactgacagt
     1141 ccacccgcct gccctggcgc gaaaaacctg ccgccagctc gttcaggagg ggaacgagat
     1201 catgccgggc catctccagc cggggctcgc gggaataggc aagaaaatcg ccgacgatcc
     1261 ggttgaggcg ggcgatctcc tcgtcgatat aatcgaagaa ctcggcgctc ttttccggct
     1321 gcgtttgctt ctcgcgcaga acatcggctg tgctcttgat gatggccagg ggattgcgga
     1381 tctcatgggc cacggtcgct gccatctgcc cgagactggc gaggcgctcg gattggtaga
     1441 gacgggtctc cgtacgcagg aactgcagca tggccaggat gagaaaaagg gccagagcag
     1501 cgagcagcag cacgctgatg ataacgccga tgtagagggc ccggtaaaac cggtccatcg
     1561 tttgcaaaaa gccggcattc gcctccaggg cgagcacggc gccgcgtccg tagacgtcgc
     1621 tcaccggggc gtaaacactc ttgaagtggt tgccggcgac ggtatgcagc cgcgaggcct
     1681 gcaccctgcc ggcgaaggcc gccctgatcg ccaggctgtc ctccatcagg taggggcggc
     1741 cgacgctctt ctccagctca gggcgggcat ccagcagaat gccgccgtcc tcgcccacca
     1801 gataggcggc ctccagctcg tggtcgagac ggatgcggtt gagcgtgccg cgtaaaagtt
     1861 ccgcctcccc tgcatcggta aagctgtgca ggccgagttc gaccagctca gcggtcagcg
     1921 cggctgtcgc tgccagcctc tggtcgaggg actcctcgag ctggcggccg atgctcttga
     1981 ggaagaagta ggaggcgcga ttgtaaaaaa gg
//
LOCUS       DAOPWU010000506         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_60865_length_2009_cov_3.664790, whole genome shotgun sequence.
ACCESSION   DAOPWU010000506 DAOPWU010000000
VERSION     DAOPWU010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60865_length_2009_cov_3.664790"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..>2009)
                     /locus_tag="PKM23_15700"
     CDS             complement(14..>2009)
                     /locus_tag="PKM23_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNY92967.1"
                     /translation="WLEEVEGERALEWVKAQNTATIAELQKHPGYAALEGKILSVLNS
                     RERIAFPSLVGEYIYNFWQDEKNPRGLWRRTLQADYFNSVPAWETVLDLDALSAAEGE
                     KWAFHGANYLYPAYDRCMISLSRGGSDAAEMREFDLVKKEFVAGGFFVPQAKGGVSWK
                     DRNTLLVWTDFGPGTLTTSGYPRQVKIWHRGTPLSGARPLFEGQQTDMSVGAGVDHQP
                     ERQYLFVNRRMTFYTGHIFVMEGDKLIRLDIPEDADFNGIFKNQMLIELKSDWSTGGR
                     TWRQGSLISIDYDRFLMGDRNFSVVFEPGERSSLAGMTTTKNALLLLKMNNVISELWR
                     YTLDEGQWRGVRVAAPNLGTIGLAATDDQSDHLFFTFTNYLEPTSLYYFPADGKNIRK
                     VKSLPAFFDAGKLMVEQLEAVSKDGTKIPFFVVRAKKARLDGARPTLLYGYGGFEVAM
                     RPGYSAVTGVAWLEQGGVYAVANIRGGGEFGPRWHQAALKENRQRAFDDFYAVAEELI
                     RRGITSPRHLGIEGGSNGGLLVGTAFTQRPDLFNAVICSVPLLDMKRYNKLLAGASWM
                     GEYGNPDLPEEWAYISKYSPYQNLAAGKKYPRVLFTTTTRDDRVHPGHARKMAAKMEA
                     LGYPFYYFENTEGGHGAGVTNEQRAKMIALEHIYLLKMLK"
BASE COUNT          382 a          599 c          632 g          396 t
ORIGIN      
        1 tcaaccggag ggcctatttg agcattttga gcagatagat atgttcgagg gcgatcatct
       61 tggccctctg ctcgttggtc acgcctgcgc cgtggccgcc ttcggtgttc tcgaaataat
      121 aaaagggata acccagcgcc tccattttcg ccgccatctt gcgggcatgg ccgggatgga
      181 cgcggtcgtc gcgcgtggtc gtggtgaaaa ggaccctggg gtatttcttg ccggccgcga
      241 gattctgata gggagagtac ttgctgatat aggcccactc ctccggcagg tcgggattgc
      301 cgtactcccc catccagctg gcgccggcca gcagcttgtt gtagcgcttc atgtcgagca
      361 gggggaccga acagatcacc gcgttgaaga gatcggggcg ctgggtgaag gcggtgccaa
      421 ccaggaggcc gccgttgctg ccgccctcga tgccgagatg gcggggcgag gtgatgccgc
      481 gccggatcag ctcttcggcg accgcataga aatcatcaaa ggcgcgctgg cggttctcct
      541 tgagggcggc ctggtgccac ctggggccga actccccgcc gccgcggata ttggccaccg
      601 cataaacacc cccctgctcc agccaggcga ccccggtgac ggccgaatag ccgggccgca
      661 tcgccacctc aaatccccca tagccgtaga gaagagtcgg ccgggcgcca tccagccggg
      721 cctttttggc ccgcacgacg aaaaagggga ttttggtgcc gtccttcgag actgcctcga
      781 gctgctcgac catcagtttg ccagcgtcga aaaaggcagg caggctcttg accttcctga
      841 tatttttgcc atcggccgga aagtaataga gcgaggtcgg ctcgagataa ttggtgaagg
      901 tgaagaataa atggtcggac tgatcgtccg tcgccgccag gccgatggtg ccgagattgg
      961 gggccgcaac cctaaccccg cgccattggc cctcgtcgag ggtatagcgc cacagctcgc
     1021 tgatgacgtt gttcattttc agcaggagaa gggcattctt ggtggtggtc atcccggcca
     1081 gactcgaacg ctcacccggc tcgaaaacga cggagaaatt gcggtcaccc atcaggaacc
     1141 tgtcatagtc gatgctgatc agggagccct gccgccaggt gcgaccgccg gtgctccagt
     1201 cggatttgag ctcgatcagc atctggttct tgaaaatgcc gttgaaatcg gcatcctccg
     1261 gtatatccag cctgatcagc ttgtcgccct ccatgacaaa gatatggccg gtgtagaagg
     1321 tcatgcggcg gttgacgaag aggtactggc gttcgggctg gtggtccact cccgccccga
     1381 cgctcatatc ggtctgctgc ccctcgaaaa gagggcgcgc cccgctcaag ggggtgccgc
     1441 ggtgccagat cttgacctgg cgcggatagc ccgaggtggt gagggtgccc ggaccgaaat
     1501 cggtccagac cagcagggta ttgcggtctt tccaggagac gccccccttg gcctggggga
     1561 caaaaaatcc gccggcgacg aactcttttt tcaccaggtc gaattcccgc atctcggccg
     1621 cgtcgctgcc gccgcgggag agagagatca tgcagcggtc gtaggcggga taaagatagt
     1681 ttgccccatg gaaagcccat ttttccccct cggcagcgct gagggcgtcg aggtcgagca
     1741 cggtctccca ggctggaacg gagttgaaat aatcggcctg cagggtgcgg cgccagaggc
     1801 cgcgcggatt cttttcatcc tgccagaaat tgtagatata ctcccccacc agagagggga
     1861 aagcaatgcg ctcccgggaa ttgagcacac tgaggatttt accctcgagg gctgcgtagc
     1921 cggggtgctt ttgcagctcc gcgatggtcg ccgtattttg ggccttgacc cactccaggg
     1981 cccgctcgcc ttcgacctcc tcgagccag
//
LOCUS       DAOPWU010000507         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_61155_length_2002_cov_3.970211, whole genome shotgun sequence.
ACCESSION   DAOPWU010000507 DAOPWU010000000
VERSION     DAOPWU010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61155_length_2002_cov_3.970211"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1030)
                     /locus_tag="PKM23_15705"
     CDS             complement(137..1030)
                     /locus_tag="PKM23_15705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY92968.1"
                     /translation="MRALNKNTVWGYLFASPWIVGFLLFGLYPILVSFWYSLCQYDVL
                     RIPQYIGLRNYRELLFEDHYFYTAVWNTLFYTLVRTPLVIVGSLLLAMLVSKAVRGVR
                     LFRTIYFIPSIISGVILSVLWMWLLNPEYGLVNSTLQFFGLQGPLWLIDPRWSKPAII
                     LMSVWSLGGGRMLVFLAAIQNVPKHFYEAVEMDGGGWWARFRHVTLPFISPVLFLWFI
                     FEIIGSLQVFVEAYVMTKGGPLNSTLFYNLYLYNKAFDDFDMGYASALAWLLFILAGI
                     ITLIQFRVSRKWVYYSGGVGK"
     gene            complement(1030..>2002)
                     /locus_tag="PKM23_15710"
     CDS             complement(1030..>2002)
                     /locus_tag="PKM23_15710"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY92969.1"
                     /translation="TRFFPSLWQEMSYRGRTYALPAYTGSYALFYNRRLLREAGLDPD
                     RPPANWQEIQDYSRRLTRRENGRIVRMGFIPQYGTIETPLIIAFEQGARFIDPAGTRV
                     HLTTPEVVSALQWERDFFDLYTVEEVSAFMGGFGYGNQQGFIAEKVALFINDNSFIDQ
                     IARYHPELDYGVAMIPAWGEGPSASSAGSWWLAIPRGAKNREDAWTFMKFVVSRETQL
                     AESMSRDETLFPVNRFAANDSDFLGMHRYMAIFSRQMEQTRSKSIVPLVHDIFWREFS
                     LARERSLRGVMSPEQALGQAERTIQAFLDRALEYDRYVDARMETTEF"
BASE COUNT          453 a          580 c          643 g          326 t
ORIGIN      
        1 ggccagaggc tgcgcgaaaa aacctccgcc tccggcttca aggaggtgag caggatccag
       61 acgaagggca ggaggaaaaa gagggcgatg gccgccatca ctccgacgat gagataatcc
      121 tgatgcctcc ggccgctcat ttgcccaccc cgccgctgta atagacccat ttcctgctca
      181 cccggaactg aatgagggtg ataatccccg ccaggatgaa gagcagccag gcgagggccg
      241 aggcatagcc catatcgaaa tcgtcaaagg ccttgttgta gaggtagaga ttgtaaaaga
      301 gggtggaatt gagcgggccg cccttggtca tgacgtaggc ctcgacgaag acctgaaggg
      361 agccgatgat ctcgaagatg aaccagagaa agagcaccgg cgagatgaag gggagggtga
      421 cgtggcggaa gcgcgcccac cagccgccgc cgtccatctc caccgcctca taaaaatgct
      481 tcggaacatt ctggatggcc gcgaggaaga ccagcatgcg cccgccgccc agcgaccaga
      541 cgctcatgag tatgatcgcc ggcttggacc agcgcggatc gatcaaccag agcggcccct
      601 gcaagccgaa gaactgcagc gtgctgttga cgagcccgta ttcgggattg agcagccaca
      661 tccacagcac cgagaggatg acccccgaga tgatcgatgg gatgaaatag atggtgcgga
      721 agagcctcac cccgcgcacc gccttgctga ccagcatcgc cagcagcagc gatccgacga
      781 tgaccagcgg ggtgcgcacc agggtgtaaa agagggtgtt ccagaccgcg gtgtagaaat
      841 agtggtcctc aaagagcagc tcgcgatagt tgcgcaggcc gatgtactgg ggaatgcgca
      901 gcacatcgta ctggcagagg ctgtaccaga atgacaccag gatgggatag agtccaaaga
      961 gcagaaagcc aacaatccag ggcgaggcga agaggtaacc ccacaccgta tttttgttca
     1021 atgctctcat caaaactccg tggtttccat gcgcgcatcg acatagcggt cgtactcgag
     1081 cgcccggtca agaaaggcct gaatggtgcg ctccgcctgc cccagggcct gctccgggct
     1141 catcactccg cgcagggaac gctcgcgcgc cagcgagaac tcgcgccaaa agatgtcatg
     1201 caccagaggc acgatcgatt tggagcgggt ctgctccatt tggcggctga aaatggccat
     1261 gtagcggtgc attcccagaa agtcggagtc gttggcggcg aaacggttga ccgggaagag
     1321 ggtctcgtcc cggctcatgc tctccgccag ctgggtctcg cggctgacga cgaatttcat
     1381 aaaggtccag gcatcctcac ggttcttcgc cccgcgcggg atcgccagcc accagctgcc
     1441 cgctgacgag gccgaggggc cctcccccca ggcggggatc atcgccaccc catagtcgag
     1501 ctccggatgg tagcgggcga tctggtcgat gaagctgttg tcgttgatga agagggcgac
     1561 cttttccgcg atgaagccct gctggttgcc atatccgaaa ccgcccatga aagccgagac
     1621 ctcctcgacc gtgtagaggt cgaaaaagtc gcgttcccac tgcaaggcgc tcaccacctc
     1681 gggggtggtc agatgaaccc gggtcccggc gggatcgata aagcgggccc cctgctcaaa
     1741 ggcgatgatg agcggggtct cgatggtgcc gtactggggg atgaagccca tgcgcacgat
     1801 gcggccgttc tcccgcctgg tcagcctgcg ggagtagtcc tgaatctcct gccagtttgc
     1861 aggcggacgg tcggggtcaa ggccggcctc gcgcaggagg cggcggttgt aaaagagggc
     1921 atacgatccg gtataggccg gcagggcata ggtgcggccg cggtagctca tctcctgcca
     1981 gagagagggg aaaaaccggg tg
//
LOCUS       DAOPWU010000508         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_61601_length_1992_cov_4.212700, whole genome shotgun sequence.
ACCESSION   DAOPWU010000508 DAOPWU010000000
VERSION     DAOPWU010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61601_length_1992_cov_4.212700"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PKM23_15715"
     CDS             <1..1439
                     /locus_tag="PKM23_15715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNY92970.1"
                     /translation="LTTYLTRQSQFIDARTALEQSATDLYGSTETAAVSSAFDAVKVT
                     KSGGSGGGTTPRGAGENEVNVTTGGSQWIAFVGDDTRLGLYDVANDQDYLLNITVNSK
                     EYNWTQFTATADGYYLYFINSEEVLSRLDLTNLAGGYYNYETFEEYYIRNPGDLWNAA
                     VSRDGEYVALTSIYENDNTIYLLISGELYYVTLEFPSSQTGITSQTIQYPDVLNWSPN
                     TKYPKLAFDAYNQITLDNGTERSWWNMGEIDFTGSELQVYTLLPAQPEGISVGNVQYS
                     STDPDRIAYSYIEDGNNYWDVKIENFASHDNDDFLQLSGRDIERPSFSPDDRYLVVDR
                     YSDEKLLIIDLVERSFWALDFENTTGARYPEWFVVGGSYDLEVEASPAAQPAGFVLEA
                     NYPNPFNAGTTISYTLAQSGRVRVAVYDLQGRLVRTLSDQPQAAGRHTLTWHGDGNDG
                     RTLSSGVYLCRVEAEGGYSASQKMVMVK"
     gene            complement(1512..>1992)
                     /locus_tag="PKM23_15720"
     CDS             complement(1512..>1992)
                     /locus_tag="PKM23_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005978520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNY92971.1"
                     /translation="TMASAVAYVVYTWLLAPTQVNIFYTLFGLKKPPDLTFLNTIVFI
                     LVIAALVQFVEMVVQKTSPSLYKSLGIYLPLITTNCAVLGVTLINVNEKYDFVECSVH
                     GFAAGIGFTIALVLMAGIREKLQLADVPKAMRGLPITFIMAGLIALAFLGFSGLKIN"
BASE COUNT          442 a          610 c          566 g          374 t
ORIGIN      
        1 ccctgaccac ctacctcacc cgtcagtccc agttcatcga tgcccgcacc gccctggagc
       61 agagcgccac cgatctttac ggcagcactg aaaccgcagc cgtcagttcg gcctttgatg
      121 cggtcaaggt caccaagagc ggcggcagcg gcggcggcac caccccgcgc ggcgccggcg
      181 agaacgaggt caacgtcacc accggcggca gccagtggat cgccttcgtc ggtgacgaca
      241 cccgcctcgg cctctacgac gtcgccaatg accaggacta cctgctcaac attaccgtca
      301 acagcaagga atacaactgg acgcagttca ccgccaccgc cgacggttac tatctctact
      361 tcatcaattc cgaggaggtg ctctcccggc tcgatctcac caacctcgcc ggcggctatt
      421 ataactatga gaccttcgag gagtattata tccggaatcc gggcgacctc tggaacgccg
      481 cggtctcgcg cgacggcgag tatgttgcgc tgacctccat ctatgaaaac gacaatacga
      541 tctatctgct gatctcgggc gaactatact acgtcaccct cgagttcccc tcttcgcaga
      601 ccggcatcac cagtcagacc atccagtatc cggatgtact caactggtcg cccaatacca
      661 agtaccccaa gctcgccttc gatgcctata accagatcac cctcgacaac ggcacggaac
      721 ggagctggtg gaacatgggc gaaatcgact ttaccggcag cgagctgcaa gtttacaccc
      781 tcctgcccgc acaacccgaa gggatcagcg tcggcaacgt acagtacagc tccaccgacc
      841 ctgaccgcat cgcctacagc tatatcgaag atggcaataa ctactgggat gtcaagatcg
      901 agaatttcgc atcgcacgat aacgatgatt ttctgcagct ctcgggccgt gatatcgaac
      961 gtccctcctt ctcgccggat gaccgctatc tggtagtcga ccgttacagc gatgaaaagc
     1021 tgctcatcat cgatctcgtc gagcggagct tctgggcgct cgatttcgag aacaccaccg
     1081 gggcgcgcta tcccgaatgg ttcgtcgtcg gcggcagtta tgacctcgag gtcgaggcgt
     1141 cacctgcggc ccagcccgcc ggttttgtcc tcgaggccaa ctatcccaat cccttcaacg
     1201 ccgggaccac gatcagctac actctggcgc agagcggccg tgtgcgcgtg gccgtctatg
     1261 acctgcaggg ccgtctggtg cgcaccctga gcgatcagcc ccaggcggct gggcgccaca
     1321 ccctgacctg gcacggcgac gggaatgatg gccgcaccct ctcctcgggg gtctatctct
     1381 gccgggtgga ggccgagggc ggttacagcg ccagccagaa gatggtgatg gtcaaatagc
     1441 ctccatccaa cggaataaaa aaaaagccag ggagcgatgc tctctggctt tttttgtggc
     1501 agaaatttaa atcagttgat cttcagcccc gagaagccga ggaaggcgag ggcgatcaag
     1561 ccggccatga tgaaggtgat gggcaggccg cgcatcgctt tgggcacatc cgccagctgg
     1621 agcttttcgc ggatgccagc catcaacacc agggcgatgg tgaagccgat ccccgcggca
     1681 aagccgtgca cgctgcattc gacaaagtca tacttttcat tgacgttgat cagagtgacg
     1741 ccgaggaccg cacagttggt ggtgatcagc ggcagataga tgcccaggga tttgtagagg
     1801 gacgggctgg tcttctggac caccatttcg acgaactgga cgagtgcggc gatgaccagg
     1861 atgaagacaa tagtgttgag aaaggtgaga tcgggcggtt ttttcaggcc aaagagggtg
     1921 tagaagatgt tgacctgggt cggggcgagc agccaggtat agacgacata ggcgaccgcg
     1981 gaggccatgg tg
//
LOCUS       DAOPWU010000509         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_62146_length_1981_cov_4.366563, whole genome shotgun sequence.
ACCESSION   DAOPWU010000509 DAOPWU010000000
VERSION     DAOPWU010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62146_length_1981_cov_4.366563"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..668)
                     /locus_tag="PKM23_15725"
     CDS             complement(141..668)
                     /locus_tag="PKM23_15725"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNY92972.1"
                     /translation="MNKRSFTTRKVYARKPASTKTGKKSVIDTGAIKKGSKTILIWGL
                     LVINVILVASLVKKIIIPSGGTGTSIVDSSTTSVSVLNGCGVKGVALVFADALREKQY
                     NVVNTGNAETFDYEKSVLINRGQISNREVEKIAALVGVSRDRILTIESKTSQSDVDLI
                     IGSDYKDLRAYKKKH"
     gene            complement(870..1715)
                     /gene="panC"
                     /locus_tag="PKM23_15730"
     CDS             complement(870..1715)
                     /gene="panC"
                     /locus_tag="PKM23_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNY92973.1"
                     /translation="MPVVTSPAEMAAQAERWRKAGETIALVPTMGCLHEGHASLIRRA
                     RQKAGKVVVSIFVNPIQFGPNEDYASYPRDLKGDTALAAAEGADLIFAPEASALYGPD
                     FQTTISVAKLTAGLCGASRPGHFNGVTTVVALLFHLVKPHLAVFGAKDYQQAAVIRRM
                     NTDLFFGIEIDVAPIVREPSGLALSSRNSYLSPQERAEATVLFQSLRLAEGLVQAGER
                     NAGKITAAMRGLIDGVASSSIDYIAIVDPDSLAPVEEIDREVQVLLAVRIGTTRLIDN
                     GRIGR"
     gene            complement(1745..>1981)
                     /locus_tag="PKM23_15735"
     CDS             complement(1745..>1981)
                     /locus_tag="PKM23_15735"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNY92974.1"
                     /translation="ELARRVSAELEIPTIGIGAGPDCDGQVLVLYDLLGLFEEFRPRF
                     VRRYAELGEVVRDACRRYGDDVRAGKFPGPQESY"
BASE COUNT          388 a          554 c          634 g          405 t
ORIGIN      
        1 attgaagagg gccgcaagac gaacggaaaa catgaaaagg gggatgaata tcccattgta
       61 gagcagaagg atgatgggct gcatgcctgc cagctggtag gtggttgaaa gaatgcctcc
      121 cgagggggag gcatccggaa tcagtgcttt ttcttgtatg cgcgcagatc cttgtaatcg
      181 gagccgatga tgagatcgac gtcgctctga gaggttttgc tctcgatggt gaggatgcgg
      241 tcgcgggaga ccccgacgag tgcagcgatc ttttcgacct cccggttgct gatctggccg
      301 cggttgatca ggaccgattt ctcatagtcg aaggtctcgg cattgccggt attgacgacg
      361 ttgtactgtt tttcacgcag agcatcagcg aagaccaggg ctacgccctt gaccccacag
      421 ccgttcagca ccgaaaccga ggtggtgctg ctgtcaacga ttgaggtacc ggtgccgccc
      481 gaagggatga tgatcttttt gaccagggaa gcgacgagaa tgacattgat caccagcagc
      541 ccccagatca gaatggtttt ggatcccttt ttgatcgctc ccgtatcgat gacgctcttc
      601 ttgcccgttt ttgtgctcgc aggcttgcgg gcatatacct tcctggtagt aaacgatctc
      661 ttgttcacag ccttcttcct tcccttttac cttggtgttg cggcgctgtc cccgccggct
      721 ttacggctta acaccattaa agatataata attaaccaga aaaacaacaa ttaatttcag
      781 tattttagcc cccgaaaaga ccattttccg ggcgaagccg cgcctgcagc cgggcagtcg
      841 gcccgagcgg attgcggcgt gggccgggtt tagcgcccga tgcgcccatt gtcaatcagc
      901 cgggtggtgc cgatgcgcac ggcgagcagg acctgaacct cccggtcgat ctcctccacc
      961 ggcgcgaggc tgtcggggtc gacgatagcg atataatcga tgctgctcga ggccacgccg
     1021 tcgataagcc cgcgcatggc ggcagtgatt tttcccgcat tgcgctcacc ggcctgcacc
     1081 aggccctcgg ccaagcgcag ggactgaaag agaacggtgg cctcggcgcg ctcctggggc
     1141 gagagatagc tgttgcgtga acttagagcc aggccggagg gttcgcgaac gatgggggcg
     1201 acatcaattt caatgccgaa aaagagatcg gtattcatgc gccggatgac agcggcctgc
     1261 tggtaatcct tggctccgaa gacggccaga tggggtttaa ccagatgaaa gaggagggcg
     1321 accacggtgg tgacgccgtt gaaatggccg ggccgggagg ctccgcacag ccccgcggtg
     1381 agtttcgcca cgctgatggt ggtctggaaa tcggggccat agagggctga ggcttcgggg
     1441 gcgaagatga gatcggcccc ctcggcggcc gccagggcgg tatcgccctt gaggtcgcgc
     1501 gggtaggagg cgtaatcctc attggggccg aactggatgg ggttgacgaa gatgctcacc
     1561 accaccttgc cggctttctg gcgcgcgcgg cggatgaggc tggcatgtcc ttcgtgcagg
     1621 cagcccatgg tcgggaccag ggcgatggtc tcgccggcct tgcgccagcg ctcggcctgg
     1681 gcggccattt cggccgggct ggtcactacc ggcagcataa tttcttccat ttcagctccc
     1741 gtgttcaata gctctcctgc ggaccgggaa attttccggc gcgcacatca tcaccgtagc
     1801 ggcggcaggc gtcgcgaacg acctcgccca gttcggcata gcggcggacg aaacggggcc
     1861 tgaactcttc aaagaggccg agcaggtcgt agaggaccag cacctggccg tcgcagtcgg
     1921 gaccggcgcc gatgccgatg gtcgggatct ccagctcggc ggagaccctg cgtgccagtt
     1981 c
//
LOCUS       DAOPWU010000510         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_62624_length_1971_cov_4.497912, whole genome shotgun sequence.
ACCESSION   DAOPWU010000510 DAOPWU010000000
VERSION     DAOPWU010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62624_length_1971_cov_4.497912"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..>1971
                     /locus_tag="PKM23_15740"
     CDS             94..>1971
                     /locus_tag="PKM23_15740"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HNY92975.1"
                     /translation="MNSPSAPATYPGGSPLRLFPPPRQERQLTVHDYLRVLYRGRWVI
                     LVSFLSVLAATLYYTFTTEPEYQASAKLMLEQQSGVSQSLFDFSTMMKKETMINNQVE
                     ILKSRSLAENVLEDLRSTPEAGRLRLLGSEAGGGRNRLKEWFGRTGRPASLDDPLFFD
                     LVVENLRSQLSVAPIRNTDMIEIKVTAPSPFEAAYVTNAVAQAYQEMNKAQSQAEVRQ
                     VKNFIEDQMSKMHTELATSEEKLKTYQQQAKVVALDRETEELVRKIAEFETIYNEAAT
                     DLAAQRQRLNYINQQLAERNVDFDVETISTAPYLAELKKQIAEKEAALASYMASMIEI
                     GAYERNRNEVQIRERQVEALKKKFKEEIAKIAAAEFVDPAQLASTLFASKIEVETEIQ
                     SLIPRVEALKGIVATYNRELESLPQKSLTLARLMRTAQVDEKIYLMLQEKYQESRITE
                     VGQIGNVRIIDSAKEPKKPVSPRKKLNILLGLIAGLGLGVGIAFMLEYLDNSVRTMED
                     IERLGMTVMATIPFIKGEQTNGVFARAGEGKNDAEVRSINERLVTQLKPRSPVSEAYR
                     ALRTNIMFSSPGQPKKLFMVTSSGPQEGKSTTVANLAITFAQMGNRTLLIDADLRKPI
                     QH"
BASE COUNT          437 a          664 c          533 g          337 t
ORIGIN      
        1 caaccagttt ttcgattttg cggtccgcat cgccgccctg gtgcaggttt gggccctgat
       61 cgatttttac agggataact agtacaggca tccatgaatt ctccatcagc ccctgcaacc
      121 taccctggcg gcagcccact gcggcttttt ccgccccccc gtcaggagcg ccagctgact
      181 gtccacgact atctgcgcgt cctttaccgc ggccgctggg tgattctcgt ctcctttctc
      241 tccgttctgg cggccaccct ctactacacc ttcaccaccg aacccgaata ccaggcctcc
      301 gccaagctca tgctcgagca gcagtccggc gtcagtcagt ccctcttcga tttctccacc
      361 atgatgaaaa aagagaccat gatcaacaat caggtggaga tcctcaaaag ccgcagcctg
      421 gcggagaatg tgctcgaaga tttgcgcagc accccggaag ccggccggct gcgcctgctc
      481 ggcagcgagg caggcggggg acgcaaccgg ctgaaggagt ggttcggccg gaccggccgc
      541 ccggcctcgc tggacgatcc cctctttttc gacctcgttg tcgagaatct gcgcagtcag
      601 ctcagcgtcg ccccgatccg caataccgat atgatcgaga tcaaggtcac cgccccctcg
      661 ccctttgaag cggcctatgt gaccaacgcc gtcgcgcagg cctatcagga gatgaacaag
      721 gcccagagcc aggccgaggt gcgtcaggtc aagaatttca tcgaagacca gatgagcaag
      781 atgcacaccg aactggctac ctccgaggag aagctcaaga cctatcagca gcaggccaag
      841 gtggtcgcgc tcgaccgcga gaccgaggag ctggtgcgca agatcgccga gtttgagacc
      901 atctacaacg aagccgcaac agaccttgcg gcgcagcggc agcggttgaa ctatatcaat
      961 cagcagctcg cggagcgcaa cgttgatttc gacgtcgaaa ccatctcgac cgcgccttat
     1021 ctggcggagc tgaaaaagca gatcgccgag aaggaggcgg ccctcgcctc ctacatggcc
     1081 tcgatgatcg agatcggcgc ctatgagcgc aatcgcaacg aagtgcagat ccgcgagcgc
     1141 caggtcgaag ccctcaagaa aaagttcaag gaggagatcg ccaagatcgc cgcggccgag
     1201 ttcgtcgatc cggcccaact cgcttcaacc ctctttgcca gcaagatcga ggtcgaaacc
     1261 gaaatccagt cgctcatccc ccgcgtcgag gccctcaagg ggatcgtcgc cacctacaat
     1321 cgcgaactcg aatccctgcc ccagaagagc ctcaccctgg cgcggctgat gcgcaccgcc
     1381 caggtggatg aaaaaatcta cctcatgctc caggaaaaat accaggaatc ccgcatcacc
     1441 gaggtggggc agatcggcaa cgtccgcatc atcgattcgg ccaaggaacc caaaaagccg
     1501 gtctcgccgc gtaaaaaact caatatcctg ctcggcctca tcgccggcct cggccttggc
     1561 gtcggcattg ccttcatgct cgaatacctc gacaactcgg tgcgcaccat ggaggacatc
     1621 gagcgcctcg gcatgaccgt gatggccact atccccttca tcaaggggga gcagaccaac
     1681 ggggtcttcg cccgcgccgg cgagggtaaa aacgacgccg aggtgcgctc cattaacgaa
     1741 cggctggtga cccagctcaa gccgcgctcg ccggtctcgg aggcctaccg cgccctgcgc
     1801 accaacatca tgttttccag cccggggcag ccgaaaaagc tcttcatggt caccagcagc
     1861 ggcccgcagg agggcaaatc gaccacggtc gccaatctgg ccatcacctt cgcccagatg
     1921 ggcaaccgca ccctgctcat tgacgccgac ctgcgcaagc ccatccagca c
//
LOCUS       DAOPWU010000511         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_63079_length_1962_cov_2.414263, whole genome shotgun sequence.
ACCESSION   DAOPWU010000511 DAOPWU010000000
VERSION     DAOPWU010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63079_length_1962_cov_2.414263"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PKM23_15745"
     CDS             <1..317
                     /locus_tag="PKM23_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92976.1"
                     /translation="QKHNWSLGYGLIGLRSPGQREAQLRIGTNDAVRLWLNGEEVWRI
                     NMARDAAIDNDIVSVVLRPGLNRVLIKICNDINEWGYYFRVTDARGRGMPDIEFVPAD
                     AL"
     gene            401..1630
                     /locus_tag="PKM23_15750"
     CDS             401..1630
                     /locus_tag="PKM23_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92977.1"
                     /translation="MRILTLLLLLLPALLAAQQIEVQVPMDSSGTIAEITPELRARTG
                     LFSEVVDFLSARLFQGGDSSFVLEVAFRREGRLARSRSALTSAEALAFRQGLTHRLAL
                     TVRAPGINQEGRPGLVVRQTMMALGFYGWAVPLMLEIEGSRGSIAAYMLTSAAGFAFP
                     YYLTRNRPVTTTQTMMTTFGSTRGLLYGHLVRKMIAPDEHSEASEWGLPMAGSLIASM
                     AAFKLAETSGLELGSAELIGVMGDFGTGFGLGTAHVSDLWDHENRETAAHATLLGMTG
                     LGLAGGYLLSQREHYTRGDAYVLRMEGILGAQIMMPIVAALADKREKSYSAGAMAGGV
                     LGVGIGNWLLRDEDFDFSEGALISCGHIAGGLLAGGLTYLIDSSDNFDGLVYHSTIAL
                     GSLAGHALLYRTFKLRR"
     gene            complement(1614..>1962)
                     /locus_tag="PKM23_15755"
     CDS             complement(1614..>1962)
                     /locus_tag="PKM23_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HNY92978.1"
                     /translation="LEFKRRFDRRSAALGKKQYLTYYLIAAYPGCSDRDMQAMQRFVS
                     RELHITPEQVQIFTPTPSTWASVMYYTEKNPFTGEPLFVEKNGAAKERQKRRIVGGEG
                     RRGPGQPPPNASV"
BASE COUNT          320 a          597 c          658 g          387 t
ORIGIN      
        1 atcaaaagca caactggtcg ctcggctacg gattgatcgg cctgcgctcc cccgggcagc
       61 gggaggcgca gctgcgcatc ggcaccaacg atgcggtgcg gctgtggctc aacggggagg
      121 aggtctggcg gataaatatg gcccgcgatg ccgccatcga taacgatatc gtcagtgtag
      181 tgctgcggcc gggcctgaac cgggtgctga tcaagatctg caacgacatc aacgaatggg
      241 gatactactt ccgggtgacg gacgcccggg ggcggggcat gcccgacatc gaatttgtgc
      301 cggccgacgc cctgtgaggg cggtctgagc ggtttttcct gtcgcgcccc ttcgggtgct
      361 cccggcgagg cgcgctctca tccctttgaa tggacaagcc atgcgaattc tgaccctgct
      421 tttgctgctc ctgccggcct tgctcgcggc ccagcagatc gaagtccagg taccgatgga
      481 cagctcgggg acgatcgctg aaatcacgcc cgagctgcgc gcgcggacag ggcttttttc
      541 ggaggtggtt gatttccttt cggcccgtct ctttcagggc ggggactcct cctttgtgct
      601 cgaggtcgcc ttccggagag aggggcgcct ggccagaagc cggagtgcgc tcacatctgc
      661 ggaggctctg gccttccggc aggggctgac acacaggctg gcattgaccg tgcgcgcgcc
      721 ggggattaac caggagggta ggcccggcct cgtcgtccgc cagaccatga tggccctcgg
      781 cttttacgga tgggccgttc ctttgatgct ggagatcgag ggctcgcgcg ggagcatcgc
      841 cgcctacatg ctgaccagcg ccgccggctt tgccttcccc tactacctga cccgcaaccg
      901 cccggtcact accacccaga ccatgatgac caccttcggg tcgacgcggg ggctgctcta
      961 tggccacctg gtcagaaaga tgattgcgcc agatgaacac tcggaggcga gtgaatgggg
     1021 actgccgatg gcgggaagcc tgatcgcgtc tatggccgcc ttcaagctgg cggagacgag
     1081 cggtctggag ctgggcagtg cggagctgat cggggtgatg ggggatttcg gcaccggatt
     1141 cggacttggc accgctcacg tctcggattt atgggatcat gagaaccgcg aaacggccgc
     1201 ccatgccacc ctgctcggca tgacggggct gggactggcc gggggctatt tgctttcgca
     1261 gcgggagcac tatacccgcg gtgatgccta tgtgctgcgg atggagggga ttctcggcgc
     1321 gcagatcatg atgcccatcg tcgcggccct ggccgacaaa agggaaaagt cctattcggc
     1381 cggtgccatg gccggcgggg ttttgggtgt cgggatcggc aactggctgc tgcgggacga
     1441 ggactttgat ttcagcgagg gggcgctgat ctcctgcggg catatcgccg gcggcctgct
     1501 ggcgggcggc ctcacctatc tgatcgacag cagcgacaat ttcgacgggc tggtctacca
     1561 ttcgaccatc gccctgggca gccttgccgg tcacgccctg ctctatcgca ccttcaaact
     1621 gaggcgttag gagggggctg gcccggcccc cttcggccct cacccccaac aatacggcgc
     1681 ttctgccgct ctttggccgc cccgttcttc tcgacgaaga gcggctcccc ggtgaaggga
     1741 ttcttttccg tatagtacat cacgctggcc caggtggagg gcgtcggcgt aaagatctgc
     1801 acctgctcgg gggtgatgtg tagttccctg ctgacgaagc gctgcatggc ctgcatgtcg
     1861 cggtcgctgc agccgggata ggccgcgatc aggtagtagg tcagatactg ctttttgccc
     1921 agcgcagccg agcgccggtc gaacctgcgc ttgaactcca gc
//
LOCUS       DAOPWU010000512         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_63448_length_1955_cov_2.345263, whole genome shotgun sequence.
ACCESSION   DAOPWU010000512 DAOPWU010000000
VERSION     DAOPWU010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63448_length_1955_cov_2.345263"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKM23_15760"
     CDS             <1..217
                     /locus_tag="PKM23_15760"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HNY92979.1"
                     /translation="SIRSEADLVIFESSSPWEATEPHIWCYTDIRSEANRVVYFTDSP
                     FEADLLLFRTDIQSDAGWQNSGKAGLL"
     gene            complement(317..1297)
                     /locus_tag="PKM23_15765"
     CDS             complement(317..1297)
                     /locus_tag="PKM23_15765"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNY92980.1"
                     /translation="MKTRVFALLLFVLALPLLAQPGRGQRGNSSRLLDNLPVEPLSAE
                     EEQSLYLMREEEKLARDVYQALYARWNLAVFQNIAASEQRHADRIKMLLDRYGLKDPF
                     IDQHGLFTDAHLQELYGALVEQGSVSQLDALKVGATIEDLDIHDLVTLLEKVDNQDIR
                     RVYDNLTKGSRNHMRSFYRLIVAAGGSYSAQYVSQETLDEIISSPMENGPAGSSQGRN
                     GRNRGGRQGGDSGREGTKSIRVGNYPNPANPTTTIVYELEEPGMVELAIYNSTGQLVR
                     SAALGGQGTGEQRYHWDARDNFGHPVTSGLYFCRISTGEAATVHRMLLMR"
     gene            1677..>1955
                     /locus_tag="PKM23_15770"
     CDS             1677..>1955
                     /locus_tag="PKM23_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNY92981.1"
                     /translation="MKILSILGTRPEAIKMAPVIKELEKHPQELKSLICVTGQHRQML
                     DQVLELFGIHPDIDLNLMQPNQGLAVLTARLFETLDSVIEETRPDWILA"
BASE COUNT          417 a          537 c          547 g          454 t
ORIGIN      
        1 cagcatccgc tccgaagctg acctggtcat cttcgaatcc tccagcccct gggaggccac
       61 tgagcctcac atctggtgct acacggatat tcgcagcgaa gcgaaccggg tggtatactt
      121 caccgattca ccctttgaag ccgatctgct cctcttccgg accgatatcc agtcggatgc
      181 cggctggcaa aacagcggta aagcggggct gctgtaaaaa gtcgggccat ccttgcagcc
      241 gttcaatttc aatttgcctc ctcataaaaa aagccttcgg aatcgaaggc tttttttgca
      301 gaacgatgta gagttgtcag cgcatcagca gcatccggtg caccgtcgca gcctcgccgg
      361 tgctgatccg gcaaaaatag aggccactgg tgacaggatg gccaaaattg tcacgggcat
      421 cccagtggta acgctgttcg cctgtgccct ggccgcccag ggcagccgaa cggactagct
      481 gtccggtgct attatagatg gccaactcta ccatgccggg ttcctcgagc tcgtagacaa
      541 tggtggtagt gggattggcc ggattgggat agttgcccac gcggatggat ttcgtgcctt
      601 ctctgcccga atcgcccccc tgacgcccgc cgcggttgcg gccgtttcgg ccctggctgc
      661 tgccagcggg gccgttttcc atcggtgagg atatgatctc atcgagtgtt tcctgggaga
      721 catattgggc gctgtagctg ccgccggcgg caacaatcag gcggtaaaac gaacgcatgt
      781 gattccgtga tcccttggtc agattgtcat agacccgccg gatgtcctgg ttatcgactt
      841 tttcaagcag ggtgaccaga tcgtggatat ccagatcttc gatggttgcc cctaccttga
      901 gggcgtcaag ctgggaaacg gatccctgtt cgaccagagc gccgtaaagc tcctgaagat
      961 gggcgtcggt gaagaggccg tgttgatcga tgaaaggatc ttttaagcca tagcggtcca
     1021 gcagcatctt gatccggtca gcatgccgct gttcgctcgc ggcgatgttc tgaaataccg
     1081 ccagattcca cctggcgtaa agggcctgat agacgtcgcg cgccaatttt tcctcttcgc
     1141 gcatgaggta taggctctgt tcttcctcag ctgaaagcgg ttcgacgggc agattatcga
     1201 gcagccggga gctgttgccc cgttggcctc ttccgggctg cgccagcagc ggcagggcca
     1261 gaacaaaaag cagtaaagcg aaaactctgg ttttcatgtt gaacctccaa tggtttagtg
     1321 ttcatccaca gcctgtacaa ctattgtgcc agagaagatt gtgttgtaaa ttaacggatt
     1381 acgaatagta cgcagggtga gtggccgtgt gggctcgacc atttggtcga gcatatggcg
     1441 gtgttggtcg agatcgtgtt cgctgccgcc agaaaaggtg aattttgccc ttgatcgcgc
     1501 acaggaattt tgtttttcgg gcagcgtttg gcttgctatt tgcaatattt cacaaagtgt
     1561 tggccgtcat ctcttcctga acggcagccg aaatcggggc gtgcagaaga gggccggcgc
     1621 aatcagcttc ccgtaaatac gatttcaatt gtgcactcac agacgacagg aatcctatga
     1681 aaattctctc cattctcggc acccgtcccg aggcgatcaa gatggccccg gtgatcaagg
     1741 aactggaaaa acatccgcag gaattgaaga gcctgatctg cgtcaccggc cagcaccggc
     1801 agatgctcga ccaggtgctc gagcttttcg gtatccatcc cgacatcgat ctcaacctga
     1861 tgcaacccaa tcaggggctt gcggttctca ccgcccggct tttcgaaacc ctggacagtg
     1921 ttatcgaaga gaccagaccc gactggatcc tcgcc
//
LOCUS       DAOPWU010000513         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_63593_length_1951_cov_4.100738, whole genome shotgun sequence.
ACCESSION   DAOPWU010000513 DAOPWU010000000
VERSION     DAOPWU010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63593_length_1951_cov_4.100738"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKM23_15775"
     CDS             <1..393
                     /locus_tag="PKM23_15775"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNY92982.1"
                     /translation="ARGHILTLEEGKPVPDLPVITGPGLRFNSKSNRLDGYFFNQAME
                     LIDEVDNRSDLLSRQLSEVYCDPELGLVAYFSQTGMLPVLLGQDDLKAKARNLDLFFS
                     ELGPSTLMGQIRCLDARLASQIIVKKKS"
     gene            421..1656
                     /gene="ftsA"
                     /locus_tag="PKM23_15780"
     CDS             421..1656
                     /gene="ftsA"
                     /locus_tag="PKM23_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNY92983.1"
                     /translation="MDAVDIIAGLDIGTTKIGCFIAEVNEAQEIKIIGVGTAPSEGLR
                     YGIVVDSEKTVRSIRQAVEKAEEMADVQVDMVYAGIAGDHIRSINGRGVVAISGEDNE
                     VSEEDVRRVIEAARAVALPLDREVIHILPQEFIVDDQRGIRDPVGMAGVRLEAEVHIV
                     TGAIASAQNIYRCIEKAGYSTASLVLEPLASSYSVLSEDEKELGVILVDIGGGTSDIA
                     LFYEGCIRHTAVVALGGRNVTNDLAIMLRTPIESAERLKIDHGHALHCEGDKEMVVEV
                     PGVNGRPSRRISRGLLVDIIQPRMVEILTIIYEEIKKSTYVNLMTTGLVLTGGASLLP
                     GTMELAEEIFNMPVKIGHPTGFTGMIAEAGTPQCATGVGLIQYALKHRGEEGPDYSKE
                     KKSFSSLYEKFKQLFDRKL"
BASE COUNT          405 a          563 c          612 g          371 t
ORIGIN      
        1 gccagaggcc atattcttac cctcgaggag ggcaagccag ttcctgatct gccggtgatc
       61 accggccccg gcctgcgctt caacagcaaa agcaaccgac tcgacggtta tttctttaac
      121 caggccatgg agctgatcga cgaggtagac aaccgcagcg acctgctcag ccgtcagctc
      181 tccgaggtct attgtgatcc cgagctcggc ctggtcgcct atttttcgca aaccgggatg
      241 ctgcccgtgc tgctcgggca ggatgatttg aaggctaagg cgcgcaacct cgacctcttt
      301 ttcagcgagc ttgggccctc cacgctgatg gggcagatcc gctgcctcga cgcgcggctg
      361 gccagccaga tcatcgtcaa gaagaaatcg tgagccgaac ccattcgtaa ggatacgtgt
      421 atggatgcag tcgatatcat tgccgggctc gacatcggta ccaccaagat cgggtgtttc
      481 atcgccgagg tcaacgaagc ccaggagatc aagatcatcg gtgtgggaac ggcgccttcg
      541 gagggtctcc gttacggcat cgtcgtcgat tccgaaaaga ccgtgcgctc catccgtcag
      601 gcggtggaaa aggccgagga gatggccgac gttcaggtcg acatggttta tgccggcatt
      661 gcgggcgacc atatccgcag catcaacggg cgcggggtgg tggccatctc aggcgaggac
      721 aatgaggtca gcgaggagga tgtccgccgg gtgatcgaag ccgcccgcgc cgtcgccctg
      781 ccgctcgacc gcgaggtgat ccatattctg ccgcaggagt tcattgtcga cgaccagcgc
      841 ggcatccgcg atccggttgg catggccggc gtgcgcctcg aagccgaggt tcatattgtc
      901 accggggcga tcgcctccgc tcagaacatt taccgctgca ttgaaaaggc gggttattcc
      961 accgccagcc tggtcctgga gcccctggcc tccagctact cggtgctgag cgaggatgag
     1021 aaggagcttg gtgtgattct ggtcgacatc ggcggcggca ccagcgatat tgccctcttt
     1081 tatgagggct gcatccgcca caccgcggtc gtcgcccttg gcggacgcaa cgtgaccaac
     1141 gatctggcca tcatgctgcg cacgccgatc gaatcggcgg agcggctcaa gatcgatcat
     1201 ggccatgccc tgcactgcga aggggacaag gagatggtcg tcgaggtgcc gggagtcaac
     1261 ggccgcccat cgcggcggat ctccagggga ttgctggtgg atatcatcca gccgcgcatg
     1321 gtcgagatcc tcaccatcat ctatgaagag atcaaaaagt cgacctacgt caacctcatg
     1381 accacgggtc tggttctgac cggcggggcg tccctgctgc ctggcacgat ggaactggcg
     1441 gaggagatct ttaacatgcc ggtcaagatc ggccatccga ccgggttcac cggcatgatc
     1501 gccgaggcgg gaaccccgca gtgcgccacc ggcgtcggtc tgattcagta tgccctcaag
     1561 catcgcgggg aggagggacc cgattacagc aaggagaaaa agtccttcag cagcttgtac
     1621 gaaaaattca aacagctctt tgaccgcaag ttgtaattcc atgaggggcg cggccatcgg
     1681 ccgacacctc aacagcctct cccccgggct tgggataggt tcggagggga gagatgcaat
     1741 aagaggagtg cagtatggga ctgacatcat ccagcagaca cgggtcaagt ctgacggacg
     1801 gtctttcaaa cacaagggag cgggccatga atcttacatt cgattttgac gataccaacc
     1861 ccaacggcgc ggttctgaaa gtggtgggcg tcggcggtgc gggcggtaat gccgtcacgc
     1921 gcatgatctc tgagggattg aagggcgtgg g
//
LOCUS       DAOPWU010000514         1950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_63652_length_1950_cov_4.985224, whole genome shotgun sequence.
ACCESSION   DAOPWU010000514 DAOPWU010000000
VERSION     DAOPWU010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63652_length_1950_cov_4.985224"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="PKM23_15785"
     CDS             <1..1309
                     /locus_tag="PKM23_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNY92984.1"
                     /translation="GDIDQPFGDTVHYKPGSKEVKIETIFFEGDRKALSEMGVNWSTF
                     YNGQIDVSGALKSAVEEGGFSVQLDIPRRIAKIDVKALFQIFDSRNIGRVLAQPQVVV
                     TSGNEGKIQVGQDFSIKTRDFAGNMMDRFISTGTILQVTPHVIENEGKVPVILLKAHV
                     ERSQAYPDVISTIIKKSEANSLVQLYDGEETLIAGLYSNEKTELRKGVPLLKDLPWYV
                     LGLRYIFGYNRYEDTQKELIIILKASIFPEVYARQSSGANSEQARATLEERLQHYRFK
                     TQPDPGDIDLSKPARINRKLETAPGSEKVPAEQEKALSQNSARPAASRTSGRANSSSA
                     GRMNPDLALRNGTVQSVQDDMLLIGWEAGFDPASLVRQTVTLVRRTGDKVTTVGKAEV
                     LRASSQKTLARRIAGQGMAELPVRAGDQAIVRFPAYTTLGEKQ"
     gene            1306..>1950
                     /locus_tag="PKM23_15790"
     CDS             1306..>1950
                     /locus_tag="PKM23_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNY92985.1"
                     /translation="MKKKISTLCALFALFLVFCQCGEDGDQLTGNEVMEDLWSEANSV
                     LADGISGLDLVATILDTSGAPLAGAAVEFTTTAGTITSRTYSDQYGEAVARITSVASE
                     TDLEVTITARIVPDEKERCAGSFLGLASRNKDGSVAYALQKSTAEPVTEARMVIGFTG
                     VSFSAQLDKSSLCADGISTAQLRISVRESSSRKGVPGAQVLVRGKILALVEDERT"
BASE COUNT          442 a          554 c          564 g          390 t
ORIGIN      
        1 gggggatatc gatcagcctt tcggcgatac cgtccactac aagccgggca gtaaggaggt
       61 caagattgag accatctttt ttgaggggga ccgcaaagcc ctctccgaga tgggcgtcaa
      121 ctggagcacc ttttacaacg gtcagatcga tgtttccgga gccctgaagt cagcggtcga
      181 ggagggcggt ttttccgtgc agctggacat cccgcgtcgt atcgccaaga tcgacgtcaa
      241 agcccttttc cagattttcg attcgcgcaa tatcggccgg gtcctcgctc agccccaggt
      301 tgtggtcact tctggcaatg agggcaaaat ccaggtcggc caggattttt ccatcaaaac
      361 cagggatttc gctggcaata tgatggatcg tttcatcagt acgggaacca tcctgcaggt
      421 caccccccat gtcatcgaga acgagggcaa ggtgccggtc atcctcctca aggctcatgt
      481 cgaacgcagc caggcttatc ccgatgtcat cagcaccatt atcaaaaaat ccgaggccaa
      541 ctcactggtg cagctctacg acggcgagga gaccctgatc gcaggtctct attccaatga
      601 aaagacagaa ttgcggaagg gcgtgcctct gctcaaggat ctgccctggt acgtccttgg
      661 cttacgctat atcttcggct acaaccgtta tgaagacacc cagaaggaat tgatcatcat
      721 cctcaaggca tcgatttttc ctgaagtcta tgccagacaa agcagcgggg ccaattccga
      781 acaggccagg gccaccctcg aggaacggct gcagcactac cgcttcaaga cccagccgga
      841 ccccggggat atcgatctct caaaaccggc caggataaac aggaagctgg aaacagcgcc
      901 gggttcggaa aaggtgccgg cggagcagga aaaggccctg tcgcaaaaca gcgccagacc
      961 tgctgcttcc cgcacgagcg ggagggcaaa cagctcgtct gctggcagga tgaatcctga
     1021 cctggccttg cgcaatggca ctgtccagtc ggttcaggac gacatgctgc tcatcggctg
     1081 ggaggcgggt tttgatcctg cttcgctggt acggcagacg gtgaccctgg tgcgccggac
     1141 cggagacaag gtgaccacag ttggcaaggc cgaagtgctg cgcgccagtt cgcaaaaaac
     1201 cctggcgcgg cgcatcgccg gacagggcat ggccgaactt ccggtgcgcg ccggcgacca
     1261 ggccattgtg cgctttccgg cttatactac cctgggagag aaacaatgaa aaagaaaata
     1321 tccactcttt gcgccctgtt cgccctcttc ctggtttttt gtcagtgtgg ggaggacggc
     1381 gatcagctga caggcaacga ggtgatggag gatttatggt ctgaagcgaa ttctgtactc
     1441 gcggatggaa tctccggact cgatctggtt gccactattc ttgatacctc cggggcgccc
     1501 ctggctggtg cggctgtaga gttcactact accgccggga cgatcacttc cagaacctac
     1561 tccgaccagt atggtgaagc agtggcccga attaccagtg tggcgagcga aaccgacctc
     1621 gaagtcacca tcaccgcccg catagtgccg gacgaaaaag agcgctgtgc cggctctttt
     1681 ctcggcttgg catcgcgaaa caaggatggc tctgtcgcct acgccctgca gaaaagcacg
     1741 gctgaaccgg ttacagaggc caggatggta atcgggttca ccggtgtcag cttttctgca
     1801 cagttggaca aatcatcact gtgcgcagac ggcatcagca ccgcccagct ccgcatctcc
     1861 gtgcgggaaa gcagcagccg aaagggagtt cccggcgcgc aggtcctggt gcggggtaaa
     1921 atccttgctc tggttgagga tgaaaggacc
//
LOCUS       DAOPWU010000515         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_63706_length_1949_cov_4.864308, whole genome shotgun sequence.
ACCESSION   DAOPWU010000515 DAOPWU010000000
VERSION     DAOPWU010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63706_length_1949_cov_4.864308"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..966)
                     /locus_tag="PKM23_15795"
     CDS             complement(202..966)
                     /locus_tag="PKM23_15795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNY92986.1"
                     /translation="MKLAYKKTGSGRPLIILHGLFGMADNWLTIAGRIARNHTVYLLD
                     QRNHGQSPHSPEFSYRILAEDLQDFIREHDLGDVRLIGHSMGGKAAMCHALHYPERVE
                     KLVIVDIAPKAYHHPFFERVLDFILALDLSRFTTRAAIEEVFAEVVPNPAVRLFILKN
                     LQRTEEGFRWRINVQSLRDNLGTIFAAIASERRFEKPTLFVRGGRSDYVLDEDEAAIR
                     ALFPAAELVTIPEGSHWLHADAEEALCRHLRDYLQC"
     gene            1141..>1949
                     /locus_tag="PKM23_15800"
     CDS             1141..>1949
                     /locus_tag="PKM23_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNY92987.1"
                     /translation="MLGDIPVLFVEGEGLAEAWEKSLISLHEKGCEIKTEYDKPDDPP
                     SKDCSMTVVIHNPLAEPMIHKDFPGGLEDLQEYVLEVLEGIKDHCIRDASAGPEDTRW
                     EYTYHQRLFNYQDPVMARSFDQIELIAQKLAQTPWSRRQQAVTWKVWEDNFCYDPPCL
                     QSLWCRLLPGEGERWRLNMNVRFRSNDAYKAAFMNMFALAQLQLKIAARIAELAGKQV
                     VPGRYVHQADSYHIYGFNLAEFKARFLHALQTRSFEGRTFRYEDVREIMEEA"
BASE COUNT          409 a          591 c          582 g          367 t
ORIGIN      
        1 tccgccgggg agaggcccgg ctgtttgagc agctccgcga tcgcgagacc ggcgggggtt
       61 aattccccct gctcgagcat ggtgcgatac tcctctttgt gcgcccggcc ggcctgggca
      121 caggccaacg ccagtgcaag gctggcgaga atgaaccatt ttccttttct cgacatcgtc
      181 tgccctcctc cggataatat tttaacactg caaatagtcg cgcaaatgac ggcagagggc
      241 ctcctcggca tcggcgtgca gccagtgcga tccctccggg atagtgacca gctccgccgc
      301 cgggaacagg gcgcggatag ccgcctcatc ctcgtccagg acatagtcgg atcggccgcc
      361 gcggacaaag agggttggtt tttcaaaccg gcgctccgat gcgatcgccg caaagatcgt
      421 cccgagattg tcgcgcaggc tctgcacatt gatccgccag cgaaagccct cctcggtgcg
      481 ctggaggttc ttgagaatga aaagccgcac cgccggattg gggacaacct ccgcaaaaac
      541 ctcctcgatc gcggcgcgcg tcgtgaaccg gctcaggtcc agagccagta tgaaatcgag
      601 cacccgttcg aagaagggat ggtgataggc cttgggggca atgtccacga tcaccagttt
      661 ttcgacccgc tccgggtaat ggagggcatg gcacatcgcc gccttgccgc ccatggagtg
      721 gccgatgagg cggacatctc caagatcatg ctcgcggata aagtcctgca gatcctccgc
      781 caggatccgg tagctgaatt ccggggaatg cggcgactga ccgtgattgc gctgatccag
      841 cagatagact gtgtgattgc gggcgatccg cccggcgatg gtcagccagt tgtccgccat
      901 cccgaagagt ccgtgcagga tgatcaacgg cctgccgctg cccgtttttt tataggcgag
      961 tttcatcgac cggcatcctc tttccctgtg tgattcaggg gcaaattagc aatcggagag
     1021 accagaagca agcacaaatt ccggcgaggc cggcgaaagt tgcttgcttt gtcagcaaag
     1081 gatttctata ttgagggcgg gccggccgga gggagaggtt aaaaaaacaa ggagaacgac
     1141 atgctcggag atattcctgt cctgtttgtt gagggcgagg ggcttgccga agcctgggaa
     1201 aaatccctga tcagcctcca tgaaaagggc tgcgagatca agaccgaata tgacaagccc
     1261 gacgatcccc cgagcaagga ctgctcgatg accgtcgtca tccataatcc cctggccgag
     1321 ccgatgatcc acaaggactt tcccggcggc ctcgaggacc tgcaggaata tgttctggag
     1381 gtgctcgagg gcatcaagga ccactgcatc cgcgacgcct ccgccggccc cgaggatacc
     1441 cgctgggagt acacctacca ccagcgcctc ttcaactatc aggacccggt gatggcccgg
     1501 agctttgatc agatcgaact catcgcgcaa aagctggcgc aaaccccctg gtcacggcgg
     1561 cagcaggcgg tcacctggaa ggtctgggag gacaattttt gctacgatcc cccctgcctg
     1621 cagagcctct ggtgccgcct cctgcccggc gaaggagagc gctggcgtct gaacatgaat
     1681 gtgcgcttcc gctccaacga cgcctacaag gccgccttta tgaacatgtt cgctctggcg
     1741 cagctgcagc tgaaaatcgc cgcccgcatc gcggaacttg ccggcaagca ggtcgtgccg
     1801 ggccgctacg tgcaccaggc ggacagctac cacatctacg gcttcaacct ggccgaattt
     1861 aaagcccgct tcctccatgc cttgcagacg cgcagctttg aagggcgcac cttccgctat
     1921 gaggatgtgc gcgagatcat ggaggaggc
//
LOCUS       DAOPWU010000516         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_63844_length_1946_cov_4.781068, whole genome shotgun sequence.
ACCESSION   DAOPWU010000516 DAOPWU010000000
VERSION     DAOPWU010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63844_length_1946_cov_4.781068"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..276)
                     /locus_tag="PKM23_15805"
     tRNA            complement(204..276)
                     /locus_tag="PKM23_15805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(241..243),aa:Thr,seq:cgt)
     gene            complement(405..794)
                     /locus_tag="PKM23_15810"
     CDS             complement(405..794)
                     /locus_tag="PKM23_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNY92988.1"
                     /translation="METPNRQQRVGRAGEEQAALYLQQQGCTVIARNFRTRHGEIDLI
                     VRDQGVLVFVEIKTCSSDAFGRPESWVTPLKQRRIAGAALTWIHLHHCEGQDCRFDVI
                     AIDLRRPEGGLQHIRNAFWMPDMEGRF"
     gene            complement(798..1427)
                     /locus_tag="PKM23_15815"
     CDS             complement(798..1427)
                     /locus_tag="PKM23_15815"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNY92989.1"
                     /translation="MKETGRAMDWLHHERELWRSGYSRIAGIDEAGRGPLAGPVVAAA
                     VIFEPEAAIIRGINDSKKLTAARREALYDQIITAALTVGIGLATHEEIDRINILMASY
                     LAMQRAVGMLIPAADFLLVDGRGLPACSEPARALIHGDSLSMSIAAASIIAKVTRDRI
                     MAGLHNAWPEYGFAGHKGYPTCEHIEAIRRFGLSPVHRRSFHPKALREE"
     gene            complement(1428..>1946)
                     /locus_tag="PKM23_15820"
     CDS             complement(1428..>1946)
                     /locus_tag="PKM23_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92990.1"
                     /translation="FRALDSLAAQCGVAVNLLADGDEFFPAQGGWLRILQTGGAGEQW
                     DENNGSMVIRLQYGGRVFLFLADIEAEGERNLLRYGSFLRSDVVKVAHHGSSTASSEG
                     LLDAVGAGWAVISVGRNNRFTLPAPGVVAHWMEKGSCVLRTDEHGAVIFRSDGEALHC
                     LRPEIAPLSAKD"
BASE COUNT          372 a          629 c          590 g          355 t
ORIGIN      
        1 ccatccatgg cgatgaaaga accgcccatg cccgcaggcc gcgctcccac ctgggtcttt
       61 aaaaattgaa accccgtggc gcccggagag tccaccgcag acgcgattcc aaccagagtg
      121 acgatcacaa taaaaaccag tacggttcgc atagtttttc ccaagtttac gccgtccacg
      181 tcacctcgcc ctgaaatagc atgagccgcc tgtcggaatc gaaccgacga cctgctcatt
      241 acgagtgagc cgctctaccg actgagctaa ggtggctttt cttggtaccc tgtattataa
      301 atcctgttaa tttaggcaac cctggccact aaatcaagca aaatctgacc tcataacgca
      361 gcccgcccct tgcgcagcgg agcagcccgg tgggtgtttc cggttcagaa acgcccctcc
      421 atgtcgggca tccagaaggc gttgcgaatg tgctgcaggc cgccttccgg ccggcgcagg
      481 tcgatggcga tgacgtcgaa acggcagtcc tggccctcgc agtggtgcag gtggatccag
      541 gtgagggcgg cgccggcgat gcggcgctgt ttgagcgggg tcacccagct ttcaggccga
      601 ccaaaggcgt cggacgagca ggttttgatt tccacaaaga ccagcacccc ctgatcgcgg
      661 acaatgaggt cgatttcccc gtgccgggtg cgaaaattgc gggcgatcac cgtgcagccc
      721 tgctgctgca ggtagagggc cgcctgctcc tcccctgcac ggccgacgcg ctgctgccgg
      781 tttggcgtct ccaaacttca ctcctccctg agggctttgg gatggaacga gcgccggtgg
      841 accggagaga ggccaaaccg gcgaatagcc tcgatgtgtt cgcaggtggg ataccccttg
      901 tgtccggcaa agccgtattc aggccaggcg ttgtgcaggc ccgccatgat gcggtcgcgg
      961 gtgaccttgg cgatgatcga ggccgcggcg atgctcatag acaggctgtc gccatgaatg
     1021 agggcgcgtg ccggctcgct gcaagccggc aatccgcggc catcaacaag gagaaaatcg
     1081 gccgcgggga tcagcatccc gacagcccgc tgcatcgcaa gataggaggc catgaggata
     1141 ttgatgcgat cgatctcctc atgggtggcc aggccgatcc cgacggtcag ggccgcagtg
     1201 atgatctggt cgtaaagggc ttcccgccgc gccgccgtga gctttttcga atcgttgatg
     1261 ccgcggatga tcgcggcctc gggctcaaag atcaccgctg cggcgacgac cggcccggcc
     1321 agcgggcccc gacccgcctc gtcgatcccg gcgatgcggc tgtatccgct ccgccagagt
     1381 tcccgttcat gatgcagcca gtccatggcg cggcctgttt ccttcactta atccttggcg
     1441 gagagcgggg cgatttcagg ccggaggcag tgcagggcct ccccgtcgct ccggaaaatg
     1501 accgcaccgt gctcatcggt gcgcagcacg cagctccctt tctccatcca atgcgccacc
     1561 actcccggcg caggcagggt gaagcggttg tttctgccga cggagatcac ggcccagccg
     1621 gctccgacgg catcgagcag cccctccgag ctggcggtgc tgctgccgtg atgggccacc
     1681 ttgacgacat cgctgcgcag gaaactgcca tagcgcagca gattcctctc tccctccgcc
     1741 tcgatgtcgg cgagaaagag gaaaacccgg ccgccgtact ggaggcgaat aaccattgaa
     1801 ccattatttt cgtcccattg ttccccggcg ccgccggttt gcaggatgcg gagccagccc
     1861 ccctgcgcgg gaaaaaattc gtccccgtca gcgagaaggt tcacggccac cccgcactgt
     1921 gctgccaggc tatccagggc gcgaaa
//
LOCUS       DAOPWU010000517         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_64065_length_1942_cov_3.475888, whole genome shotgun sequence.
ACCESSION   DAOPWU010000517 DAOPWU010000000
VERSION     DAOPWU010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64065_length_1942_cov_3.475888"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PKM23_15825"
     CDS             <1..1064
                     /locus_tag="PKM23_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNY92991.1"
                     /translation="LCLSSLRAPGPFPSGLAWAEGSLWCADFENREIYQIEVLSAEIM
                     TRWDKKELDLTFIKEFRNYGPGTVTGLDIYLPIPESRDNQKLLQPVAFKPGANEIISD
                     LWGQKLAHYRFENLASGSMVKPSWSVQAEVYAVEYFIYPDRIGPLSAIPAEIREKYLG
                     DGDKYQIGDPYIQELAKKIAGAEENPYWIARRVYEYLTEHLTYNLKPVGGWNPAPTVL
                     RRGTASCSEYSYTMIALCRALGVPVRYVGAVRLRGDDASVDDVFHRWTEVWLPPYGWI
                     PFDANKGDAETPGGKVLGIGNVANRYIITTENGGGDQYLWFGYNYGFTWRSEGKCRIA
                     EESYGLWSPLGAKKVQKSL"
     gene            complement(1283..>1942)
                     /locus_tag="PKM23_15830"
     CDS             complement(1283..>1942)
                     /locus_tag="PKM23_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015168962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNY92992.1"
                     /translation="LTIALADSILTGRSYTENLKTFYRLYPNGGYGGSFHHWAQSPSS
                     SPYNSWGNGAAMRISPVGFAFDDLETVLQKAKEYTEVTHNHPEGIKGGQATAAAIFLA
                     RSGRSKEEIRAYIESTFYYDLSWSVDEIRPGYKFNESCQETVPQAIRAFIDATSFEDA
                     IRNAISLGGDSDTLACITGGIAQAFYKGVPADIAANVYAILDERLADITRRFMAKYRI
                     E"
BASE COUNT          412 a          556 c          598 g          376 t
ORIGIN      
        1 gcctctgcct ttcctccctg cgcgcgcccg gcccctttcc cagcggactg gcctgggcgg
       61 aggggagcct ctggtgcgcc gatttcgaga accgcgagat ctaccagatc gaggtgctca
      121 gcgcggagat catgacccgt tgggataaaa aggagctcga tctcaccttc atcaaggagt
      181 tccgcaacta cggtcccggc accgtgaccg gcctcgacat ttatctgccc atcccagaga
      241 gccgcgacaa ccaaaagctg ctgcagccgg tcgcttttaa gccgggggcc aacgagatca
      301 tcagcgacct ttggggacaa aaactcgccc attaccgctt tgaaaacctg gccagcggca
      361 gcatggtcaa gccctcctgg agcgttcagg ccgaggtcta tgcggtcgag tactttatct
      421 atcccgatcg tatcggcccg ctcagcgcga tcccggccga aatcagggaa aaatatctcg
      481 gcgatggcga taaatatcag atcggggatc cctacatcca ggagctggcg aaaaaaatcg
      541 ccggggctga agaaaatcct tactggatcg cccgccgggt ctacgaatac ctgaccgaac
      601 acctcaccta caatctcaaa ccggtggggg gatggaatcc ggcgccgaca gtactgcggc
      661 gcggcaccgc ctcctgctcc gaatactcct acaccatgat cgccctctgt cgcgctctgg
      721 gtgtgccggt gcgctacgtc ggggcagtgc gcctgcgcgg cgacgacgcc agtgtcgacg
      781 atgtcttcca ccgctggact gaggtctggc tgccgcccta cggctggatc ccctttgacg
      841 ccaacaaggg cgacgccgag accccgggcg ggaaggtcct cggcatcggc aatgtcgcca
      901 accgttacat catcaccacc gagaacggcg gcggcgacca gtacctgtgg tttggctaca
      961 attacggatt tacctggaga agcgagggca agtgccgcat cgccgaagag agctatggcc
     1021 tttggtcgcc tttgggggcg aaaaaggtgc agaaatccct gtagcgcagg ccgggagagg
     1081 gggaaagagg acgaatccgg ccgcgaccgg gaaaaaaggc cgtagcggtt aaaaatcggg
     1141 gcaggatgcg agaaaagaaa tttcctgaaa tcctgatcag tggccctcaa gagcgggaga
     1201 ggtcattatc ctcaagagta taatattaca caagagccgc atccgtacgg acggcatggg
     1261 gaggcggctc ttgtgaacag ttttactcta tcctgtattt tgccatgaac cggcgggtga
     1321 tatcggcaag ccgctcatcg aggatggcat agacatttgc ggcgatatct gcgggcacgc
     1381 ccttgtagaa ggcctgggcg atgccgccgg tgatgcaggc gagggtgtcg gaatctcccc
     1441 ccagcgagat ggcgttgcgg atggcatcct caaaactggt ggcgtcgata aaggcccgga
     1501 tcgcctgggg aacggtctcc tggcaggatt cgttgaactt gtagccgggg cggatctcat
     1561 ccacgctcca gctcagatca tagtagaagg ttgattcgat atacgcccgg atttcctctt
     1621 tcgaccggcc gctgcgcgcc agaaaaatcg ccgctgcggt ggcctggccc cccttgatgc
     1681 cctcgggatg attgtgagtc acctcggtat attctttcgc cttttgcagc acggtctcca
     1741 gatcatcgaa tgcgaatccg acgggactga tgcgcatggc ggcgccgttg ccccagctgt
     1801 tgtagggcga agaggaggga ctttgcgccc agtggtgaaa gctgccgcca tagccgccat
     1861 tgggatagag gcggtagaag gttttcaggt tttcggtata ggatctcccg gtgaggatgg
     1921 aatcggccag ggcgatggtc ag
//
LOCUS       DAOPWU010000518         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_64296_length_1937_cov_6.130712, whole genome shotgun sequence.
ACCESSION   DAOPWU010000518 DAOPWU010000000
VERSION     DAOPWU010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64296_length_1937_cov_6.130712"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PKM23_15835"
     CDS             <1..751
                     /locus_tag="PKM23_15835"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNY92993.1"
                     /translation="GRGDSLDLVLGADDQLFIRRIPDWQVGPLVEVRGEVTFPGYYPI
                     VDDSTRLSEIMKNCGGFSPDALISDAKLIRRRQPMPEDREYERLRAMPRDQMSDGEYE
                     YLVMKQNSSDADRIVVDFRRLLQLNDRSQDVALRDGDQILVPKTPRVVQISGCVARPG
                     GVLYKPEADYKYYIAQAGGLSWDADGRRAKVIKASGEIKDDEDVDDLAPGDRIWVPRK
                     PDRNYWQIFRDTILVAGQIATVFLVIQNATE"
     gene            755..>1937
                     /locus_tag="PKM23_15840"
     CDS             755..>1937
                     /locus_tag="PKM23_15840"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNY92994.1"
                     /translation="MADNQDKVIEIHLLDYLLVITRWRWRILRNILVAAVGIFLLSFL
                     MPKKYIAVTTLLPPPEQEGSAMASLLSEVKVPGVALPGKVTSADIFLEMLRSRSVGER
                     VLQRRFLSRGDSTTLLEILDCKTIEGGLIKMSKRASFVLSKKMVMTISVEMGEPQLAA
                     AVANAYVEELDRVNREKSVSRAKNSRLYIESQLAETQARLGDATRLLAEYQRGHRAVS
                     LEEQVKASILQSGEVKGQIIAKEIELNVMLQSMKPENPLVVRARQELTELRRKYDDLQ
                     IGSGDRQQEEIFMPVARVPEVGIQMAELMREAKVQETVWQLLNSQYYQTRIEEARDTP
                     TVQVLDAAVPPLFKSSPKRKMLAVVLGLLAGIATIFYALCRNMPDASTNGPPKRPGGR
                     SS"
BASE COUNT          383 a          635 c          591 g          328 t
ORIGIN      
        1 ggggcgcggg gattcgcttg acctcgtgct cggcgccgac gaccagcttt tcattcgtcg
       61 catccccgac tggcaggtcg gccccctggt cgaggtgcgc ggcgaggtga ccttccccgg
      121 ctattatccc atcgtcgatg actccacccg cctcagcgag attatgaaga attgcggcgg
      181 attcagcccc gacgccctca tcagcgacgc caagctcatc cgcaggcgcc agcccatgcc
      241 cgaagaccgc gaatatgaac ggctgcgggc gatgccccgc gaccagatga gcgacggcga
      301 atatgagtat ctggtcatga agcagaacag cagcgacgcc gaccgtattg tcgtcgattt
      361 ccgccggctc ctgcagctca acgaccgcag ccaggacgtc gccctgcgcg acggcgacca
      421 gattctcgtc cccaaaaccc cgcgcgtggt gcagatcagc ggctgcgtcg cccggcccgg
      481 gggcgtgctc tacaaaccgg aagccgacta caagtactac atcgcccagg ccggaggatt
      541 gagctgggac gccgacggcc gccgcgccaa ggtgatcaag gcctccggcg agatcaagga
      601 cgatgaggat gtcgatgatc tcgctccggg cgaccgcatc tgggtgccgc gcaaacccga
      661 ccgcaattac tggcagatct tccgcgacac catccttgtc gccggacaga tcgccacggt
      721 atttctcgtg atccagaacg ccacagagtg agccatggcc gataaccaag acaaagtgat
      781 agagatccat ctcctcgatt acctgctggt gatcacccgc tggcgctggc gcatcctgcg
      841 caacatcctc gtcgccgctg tgggcatctt tttgctctcc tttctcatgc cgaaaaaata
      901 tatcgcggtg accactctgc tgccgccgcc cgagcaggag gggagcgcca tggccagcct
      961 gctctccgaa gtcaaggtgc ccggcgtcgc cctgcccggc aaggtcacct cggcggatat
     1021 ctttctcgaa atgctgcgca gccgctcggt gggggaacgg gtgctgcagc gccgttttct
     1081 ctcccggggt gacagcacga ccctgctcga gatcctcgac tgcaagacca tcgagggcgg
     1141 cctgatcaaa atgagcaagc gcgcctcttt tgtgctctcg aaaaagatgg tcatgaccat
     1201 cagcgtcgag atgggcgagc cgcagctggc cgccgcagtc gccaacgcct acgtcgagga
     1261 gctcgaccgc gtcaaccggg agaagagcgt ctcgcgtgcc aagaattccc gcctctacat
     1321 cgagtcgcag ctggccgaga cccaggcgcg actcggcgat gccacccggt tgctggccga
     1381 ataccaacgc ggccatcgcg ccgtctcgct cgaggagcag gtcaaggcct cgatcctgca
     1441 gagcggcgag gtcaaggggc agatcatcgc caaagagatc gaactcaacg tcatgctgca
     1501 aagcatgaag ccggaaaatc ccctggtggt gcgcgcccgc caggagctga ccgagctgcg
     1561 ccgcaagtat gacgacctgc agatcggctc gggcgaccgc cagcaggagg agatattcat
     1621 gcccgtggcc agggtgccgg aggtgggcat ccagatggcg gagctgatgc gtgaggccaa
     1681 ggtgcaggag acggtctggc agctgctcaa ctcgcagtat tatcagaccc ggatcgagga
     1741 ggcgcgcgac acccccaccg tgcaggtcct cgatgccgct gtgcctcccc tctttaaaag
     1801 ctcacccaag cgcaagatgc tggccgtggt gctgggcctt ttggccggca tcgccaccat
     1861 tttttacgcc ttgtgcagga atatgccgga cgcctcgacc aacggcccgc cgaaaaggcc
     1921 aggtggcagg agttctt
//
LOCUS       DAOPWU010000519         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_64883_length_1926_cov_3.328167, whole genome shotgun sequence.
ACCESSION   DAOPWU010000519 DAOPWU010000000
VERSION     DAOPWU010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64883_length_1926_cov_3.328167"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1926
                     /locus_tag="PKM23_15845"
     CDS             <1..>1926
                     /locus_tag="PKM23_15845"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNY92995.1"
                     /translation="FLYFSSANPWSERPEDLLARLPEEWLEIRAGQPAVRKDRRPKLP
                     LRLPLNSRGQFDKQGMECYYINRPFSFCLNCGVAYGSRQKSDYAKLSTLSSEGRSTAT
                     TILSMSTIHYLKQAHDLPLKARKLLSFTDNRQDASLQAGHFNDFIEIGVLRAALYRAV
                     SNAGGQGLRHDDLTHMVFQALGLPPLAWAITPGARFAEKENAEKALRNVLGYRLYRDL
                     LRGWRITSPNLEQCGLLEIRYTSLDEVCQAEDLWQERHPALASAALEERRQVVKVLLD
                     FMRRELAIKVDYLAAEFQERIQQQSNQYLITPWALDEDEKMERGMILFPRSGGDAKAW
                     GQVYLSGRSGFGIYLRRPNTFRQFNQKLTLDETERIIRDLLAILAQAGLVIAVDVSEP
                     DCPGYQLSAAAMTWHAGDGRRAFHDPIRVPHLPADGGRTNPYFLHYYQEMALQTSGFE
                     AREHTAQVPNELRQEREQRFRMAQLPILYCSPTMELGVDIDELNVVNMRNVPPTPANY
                     AQRSGRAGRSGQPAVVFTYCTTGSPHDQYFFKRPELMVSGQVAPPRMDLANEDLIKAH
                     IHAIWLAETRPRLGKSLKDILDLSGSEPALALMNDVLADLSSPEALVRTRNKAEQILA
                     GIRPELENSDWYHPGWL"
BASE COUNT          378 a          628 c          540 g          380 t
ORIGIN      
        1 gatttctcta tttcagcagc gccaatccct ggagcgaaag gcccgaggat ttgctggccc
       61 gactgccgga ggagtggctg gagatccgcg ctggccagcc tgctgtgcgc aaagaccgcc
      121 gccccaaatt gcccctgcga ttgcccttga acagcagggg gcaatttgat aagcagggga
      181 tggaatgtta ctacatcaac cgtccctttt ccttctgcct caactgcggt gtcgcctacg
      241 gcagccggca gaagagcgac tatgccaagc tttccacact ctcttcggaa ggccgcagca
      301 cggccaccac tatcctgagt atgtccacca ttcactatct caagcaagcc catgatctgc
      361 ccctcaaggc gcgcaagctg ctgagtttta ccgacaaccg ccaggatgcc tcacttcagg
      421 ccggtcattt caacgatttt atcgagatcg gcgtgctgcg cgccgccctc tatcgcgccg
      481 tgtcgaacgc aggcggccag ggcctgcggc atgacgatct gacccacatg gtttttcagg
      541 ccctcggtct gccgccgctg gcctgggcca tcaccccggg ggcacgcttc gccgaaaaag
      601 agaatgctga aaaagctttg cgcaacgtcc ttggctaccg actttatcgg gatctgctgc
      661 gcggctggcg cattacttcg cccaacctgg aacagtgcgg cttgcttgaa atccgttata
      721 cctcgctcga tgaggtgtgc caggccgaag atctctggca ggaacgccac cccgctctcg
      781 cctcggcagc tcttgaggag cgccgccagg tcgtcaaggt tctgctcgat ttcatgcgcc
      841 gcgagctggc gatcaaggtc gattatctcg ccgcggagtt tcaggaacgg atccagcagc
      901 agagcaacca gtatctcatc accccctggg ccctggacga agatgagaaa atggaacgcg
      961 gcatgatcct tttcccgcgg agcggcggag atgccaaagc ctggggccag gtttatctct
     1021 ccggacgcag cggcttcggc atctatctcc gccgtccgaa caccttcagg cagttcaacc
     1081 agaaactcac actggatgag accgagcgga tcatccgcga tctcctggcc attcttgctc
     1141 aggccggact ggtgatcgcg gttgacgtta gtgaacccga ttgccccggc tatcagctct
     1201 ccgccgcagc tatgacctgg cacgcgggcg atggccgcag ggcttttcac gatcccatcc
     1261 gcgtccccca tctccccgcg gatggcggcc ggaccaatcc ctatttcctc cactactatc
     1321 aagagatggc actgcagacc agcggttttg aggcgcgaga gcataccgcc caggttccga
     1381 atgagctgcg tcaagagcgc gagcagcgtt ttcgaatggc ccaattgccc attctctact
     1441 gctcgccgac gatggaattg ggcgtggaca tcgacgagct gaatgtggtc aatatgcgca
     1501 atgtgccgcc cacaccggcc aattatgccc agcgcagcgg cagggctggt cgcagcggac
     1561 aacccgctgt ggtttttacc tactgcacaa ccggcagtcc ccacgaccag tactttttca
     1621 agcggcccga gctcatggtc tcaggccagg tggcgccgcc gcgcatggat ctggccaacg
     1681 aagatctcat caaggcgcat atccacgcca tctggctggc cgaaacccgg ccgcggctgg
     1741 gcaagtccct gaaagatatc ctcgacctca gcggttcgga acccgccctt gcgctgatga
     1801 acgatgtgct cgctgatctc tcctcccccg aagccctggt gcgcacccgc aacaaggcgg
     1861 agcagattct tgcgggcatc cgcccggagc tggagaactc cgactggtac catcccgggt
     1921 ggctgg
//
LOCUS       DAOPWU010000520         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_65023_length_1923_cov_3.775161, whole genome shotgun sequence.
ACCESSION   DAOPWU010000520 DAOPWU010000000
VERSION     DAOPWU010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65023_length_1923_cov_3.775161"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="PKM23_15850"
     CDS             <1..1450
                     /locus_tag="PKM23_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNY92996.1"
                     /translation="DLGCLGAAINERALERQLEMLKGMGCNAIRTSHNPPAPELLDLC
                     DRMGFLVMDEAFDMWKLGKNPDDYHLDWNEWHRRDLEDLILRDRNHPSVMMWSIGNEI
                     LEQWDDSGLEMTRELAEIVRALDTTRPVTSACNDPAPYNRIVRSGALDVVGWNYHEKD
                     YLTFREQYPQGKLIASETNSAIATRGAYDMPSDSVRVWPVKWDEPLPMNADYTCSAYD
                     NCRVGWGSTHVDTWRIVRDNPHISGMFIWTGFDYLGEPTPYTWPARSSYFGLIDLAGF
                     PKDGYYFYQAEWTAKPMLHLFPHWNWEPGQLVDVWAYTNQEEVELFLNGQSLGRKAKG
                     PDTFQLVWRVPFAPGTLEAVAYKGGSRTLTKSVHTAGAPARIVLTPDRERIKANGRDL
                     SFVTVDIVDEQGVLVPDAANLVKFALQGPGSIAGVDNGHQISMESFKADHRKAFNGKC
                     LCVVQGKEEAGEILLTARSEGLQEATLQIRSE"
     gene            complement(1502..>1923)
                     /locus_tag="PKM23_15855"
     CDS             complement(1502..>1923)
                     /locus_tag="PKM23_15855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNY92997.1"
                     /translation="AEWGPVEGLEAIPAQAYETGDEIRIASPARSVQYLLIDGTVDRI
                     KTASALPGIFNLSQNFPNPFNARTTITYTLPHAAAVDLRIHDLNGREIKVLLDGQKMS
                     AGEHSLAIDTTQWPSTIYFYTLRSGSWSETRKMVMIK"
BASE COUNT          388 a          533 c          592 g          410 t
ORIGIN      
        1 cgatctcggc tgtctgggtg cagcgatcaa cgagcgcgcc cttgaacggc agctcgagat
       61 gctcaagggg atgggctgca acgccatccg gacctcgcac aatcccccgg ctcccgaact
      121 gctcgatctc tgtgaccgca tgggctttct ggtgatggac gaggcctttg acatgtggaa
      181 gctgggcaag aatcccgatg actatcatct cgattggaat gagtggcacc gccgcgatct
      241 ggaggatctg attttgcgtg atcgcaatca tccgagcgtg atgatgtgga gcatcggtaa
      301 cgagatcctc gagcagtggg atgacagcgg tttggagatg accagggaat tggcggagat
      361 cgtccgcgcc ctcgacacca cccggccggt cacatcggcc tgcaatgatc cagctcccta
      421 taaccgcatc gttcgctccg gcgccctgga tgtcgtgggc tggaactatc acgagaagga
      481 ttatctcact tttcgtgagc agtatccgca agggaaactg attgccagcg agaccaactc
      541 ggccatcgcc accagaggag cttacgatat gccctccgat tcggtgcggg tctggcctgt
      601 caaatgggac gagcccctgc cgatgaatgc tgattatacc tgctccgcct atgacaactg
      661 ccgggttgga tgggggtcaa cccatgtcga tacctggcgc attgtccggg ataaccctca
      721 tatctccggc atgttcatct ggaccggctt tgattatctc ggcgaaccga ccccctatac
      781 atggccggcg cgcagttcct atttcgggct gatcgatctg gcgggatttc ccaaggatgg
      841 ctattacttt taccaggctg agtggactgc aaagccgatg ctgcatctct tcccgcactg
      901 gaactgggaa ccgggccaac tggtggacgt ctgggcctac accaatcagg aggaggtaga
      961 gctgttcctc aacggtcaat ccctgggaag aaaggccaag gggccggaca ccttccagct
     1021 ggtctggcgg gtgccctttg cgcccggtac cctggaggcg gtggcgtaca agggcggcag
     1081 ccgcacactg acaaaaagcg tccacactgc cggagcgccg gcaaggatcg ttctgactcc
     1141 cgaccgggag aggatcaagg cgaatggccg cgatctctcc ttcgtcacgg tcgatattgt
     1201 cgatgagcag ggagtcctgg tgccggatgc cgccaatctg gtgaaattcg ccctgcaagg
     1261 cccaggctct atcgccggag tggataacgg tcatcagatc agcatggagt ccttcaaggc
     1321 ggatcaccgc aaggccttca acggcaagtg cctgtgcgtc gttcagggga aggaggaggc
     1381 aggcgagatt ctgctgactg cccgctcgga gggtctgcaa gaggccacac tgcagatcag
     1441 aagcgaatag gcataaaaaa agccctggct gctgccaggg cttttttttg cttcgggcgg
     1501 tttacttgat catcaccatt ttcctggtct ccgaccagct tccactccgc agggtgtaaa
     1561 agtagatggt gctgggccat tgtgtggtat cgatggccag gctgtgctcg ccggcgctca
     1621 ttttttggcc gtcgaggagc actttgatct cgcggccgtt gagatcatgg atgcggaggt
     1681 cgacagcggc ggcatgcggc agggtatagg tgatggttgt acgcgcgttg aaaggattag
     1741 ggaagttctg gctcagattg aaaatcccgg ggagagcact cgctgtttta atcctgtcga
     1801 cggtcccgtc aatgaggaga tactggacgg aacgggcggg tgacgcaatc cggatctcat
     1861 ctccggtttc gtaggcctgc gcgggaatag cttccagacc ttcgaccggt ccccattccg
     1921 ccc
//
LOCUS       DAOPWU010000521         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_65533_length_1913_cov_5.100646, whole genome shotgun sequence.
ACCESSION   DAOPWU010000521 DAOPWU010000000
VERSION     DAOPWU010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65533_length_1913_cov_5.100646"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PKM23_15860"
     CDS             complement(<1..1213)
                     /locus_tag="PKM23_15860"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513920.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HNY92998.1"
                     /translation="MGVHPLAGKPAPQELLINIPRLVSAYYTRHPDPAQPGQRVSFGT
                     SGHRGVSSAGTFNEEHLLAISQAICDYRRRQGIDGPLFLGMDTHALSEPAFITAVEVM
                     AANGVMLIVQRGRGYLPTPVISHAILTYNRGRSSGLADGVVITPSHNPPTDGGFKYNP
                     PHGGPADSQATRVIQDEANRLLQEGLAGIRRIPFARAWAAESTREQEVIRPYIADLGG
                     VVDLAAVKGAGLQLGVDPMGGASVALWESIAEYWQLKLEVVNRVVDPAFGFMTVDKDG
                     KIRMDCSSPYAMAGLVAMRDRFDIAFGNDPDADRHGIVCRSAGLMNPNHYLAVAIWYL
                     FQHRPEWPAEAAVGKTLVSSSMIDRVAASLGRRLAEVPVGFKWFVEGLLDGSCGFGGE
                     ESAGASFLRRAG"
     gene            1381..1584
                     /locus_tag="PKM23_15865"
     CDS             1381..1584
                     /locus_tag="PKM23_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY92999.1"
                     /translation="MIKLLLWLLVLFCCWPLALLALILYPLVWLLLLPFRILGIAVEG
                     LLDFLRALFGLPARILRGPQPAR"
     gene            1700..>1913
                     /locus_tag="PKM23_15870"
     CDS             1700..>1913
                     /locus_tag="PKM23_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93000.1"
                     /translation="MKIQIHKIQVDKWCEGCRLQADPGPTYVLDWIQNNGPWFRESYE
                     VSICKECKHWARCGHNLQRTCPGFEPE"
BASE COUNT          364 a          618 c          561 g          370 t
ORIGIN      
        1 ggccagcccg gcgcagaaac gaagcccccg cgctctcctc gccgccaaaa ccacaggagc
       61 cgtccagcag cccctcaaca aaccatttaa agccgaccgg cacttcggcg agccggcggc
      121 cgagcgaggc ggcgacccgg tcgatcatcg agctcgagac cagggtcttg cccactgccg
      181 cttcggcggg ccactccggc cggtgctgga agagatacca gatcgcgacg gccaggtagt
      241 ggttggggtt catcagcccc gcactgcggc agacgatgcc atgacggtcg gcatcgggat
      301 cgttgccgaa ggcgatatcg aaacggtcgc gcatcgccac cagccctgcc atggcgtagg
      361 gcgaggagca gtccatgcgg atcttgccgt ccttgtccac cgtcataaat ccgaaagccg
      421 gatcgacgac ccggttcacc acctcgagtt tgagctgcca gtattccgcg atggactccc
      481 agagtgcaac cgaggcgcct cccatcggat ccactcccag ctgcagcccc gcccccttaa
      541 ctgccgccag atcgaccacc ccgcccagat ccgcgatgta gggccggatc acctcctgct
      601 cgcgcgtgct ctccgccgcc caggcccggg cgaaggggat gcgcctgatc ccggccagtc
      661 cctcctgcag cagccggttg gcttcatcct gaatgaccct ggtcgcctga ctgtcggccg
      721 ggccgccgtg cgggggattg tacttgaagc ccccgtcggt cggcggattg tgtgaagggg
      781 tgatcacaac gccgtcggcc agcccgctgc tgcggccgcg gttgtaggtg agaatggcgt
      841 gtgaaatcac cggggtgggg agatagccgc ggccccgctg cacgatcagc atcacgccgt
      901 tggccgccat cacctccacc gcggtgatga atgccggctc ggaaagggcg tgggtgtcca
      961 tgcccagaaa aagcgggccg tcaataccct gccgccggcg gtagtcgcag atcgcctggc
     1021 tgatggccag cagatgctcc tcattgaagg tccccgcgct ggaaacgccg cggtggcccg
     1081 aggtgccgaa gctgacccgc tgcccgggct gggccgggtc cgggtgacgg gtgtaatagg
     1141 cggacaccag tcggggaata ttgatcagca attcttgcgg agccggtttg ccggccagcg
     1201 gatgaacgcc catattcgcc tccctcaaag gttctgtgca tgccggattc agggcctgta
     1261 ccggcaaaac atacaaattt cagaccctct ctacaagaaa aaagaggaaa aaggagctga
     1321 ccaggaacat ccggtcgtgt ttttcgtaat aacaaacaga acgaaccggg aggtttccac
     1381 atgataaaat tgttgctctg gctgcttgtt ttattctgct gctggccgct cgccctgctg
     1441 gcgctgatcc tctatcccct cgtctggctg ctcctgctgc ctttccgcat cctcggcatc
     1501 gccgtggagg ggctgctcga ttttctgcgc gccctctttg gactgcctgc ccgcattctg
     1561 cgcggtcccc aaccggcgcg ctgatttttc gccggcggct gcactttttc ctttcttttt
     1621 tgaaaaatag ttatatatta ttatcgtcct gaaacggttc atttttatga gtcgagttcg
     1681 cagaatgcaa gacaatttca tgaagatcca gatccacaag atccaggtcg acaaatggtg
     1741 tgaaggctgc cgcctgcagg ccgaccccgg cccgacctac gtccttgact ggatccagaa
     1801 caatggcccc tggttccgcg aatcctacga ggtctcgatc tgtaaagagt gcaaacactg
     1861 ggccaggtgc ggccacaacc tgcagcgcac ctgccccggc tttgaacctg aat
//
LOCUS       DAOPWU010000522         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_65888_length_1906_cov_4.531605, whole genome shotgun sequence.
ACCESSION   DAOPWU010000522 DAOPWU010000000
VERSION     DAOPWU010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65888_length_1906_cov_4.531605"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="PKM23_15875"
     CDS             complement(<1..1471)
                     /locus_tag="PKM23_15875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY93001.1"
                     /translation="MADQYGAFRSYARKLLSAESRRREFSPTGPTPLALVYPNSYATG
                     MSSLGYQTVYRLLNEQPLLKGERAFQLEGPFAAFYHTLESQQPLNQFRIIAFSLAYEM
                     DYPLVLQMLKRAGIPLLARERTRRDPLILMGGVAAFYNPTVMAPAADLFLIGEGEEMI
                     PQFARAYEEHLDEGEGREELLERLARIEGAWVPAVHGLQPESGRVRRRYIDIKKHPPA
                     SSICVTPNTHLEMFMVEVGRGCGRGCRFCAAGNVYHPFRSWPLEEIITEVDRHAHPGD
                     RIGLVGAALSDYRRLDQLCEQLLGRGHKISLSSLRADRISPMLLQSLAASDIRTVTLA
                     PEAGTERLRRVIRKDLDEEQILAAVDLIGRFQFRQIKLYFMIGLPTEQPEDLQGVVDL
                     TRKAAALFCRRGSGRELRVSINTFVPKPWTPFQWAGMATEREIRAKRRSVLEPLNRIE
                     GVSVGRKSGREEVLQALFSIGTVEVGEALVRAVQEKREWQ"
     gene            complement(1522..>1906)
                     /locus_tag="PKM23_15880"
     CDS             complement(1522..>1906)
                     /locus_tag="PKM23_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93002.1"
                     /translation="SSNYRRVRDAGTRTSTQFEQSGLLQKEIPAVTRLEEINNHVNRE
                     ASRDILNPKWQQRTTQPAAPARTPQQEPRSEEQPAASRPEPAYTWKDVERTLQDVQVT
                     SGNGNGHEDTEFDIPACRRNRWKLL"
BASE COUNT          331 a          599 c          629 g          347 t
ORIGIN      
        1 cctgccactc ccgcttttcc tgcaccgccc gcaccagggc ctcgccgacc tccacagtgc
       61 caattgaaaa gagggcctgg agcacctcct cacgtccgct tttacggccg acgctcaccc
      121 cttcgatgcg gttgagaggc tcgaggaccg agcgccgttt ggccctgatc tcccgctctg
      181 ttgccatgcc cgcccactga aagggggtcc agggcttggg gacaaaagtg ttgatgctga
      241 cgcgcagttc ccgcccactc ccccggcggc aaaagagggc agccgccttg cgggtgaggt
      301 cgaccactcc ctgcaggtct tccggctgct cggtcggcag accaatcatg aaatagagct
      361 tgatctggcg gaactggaac cggccgatga ggtcgacggc ggcgaggatc tgctcctcgt
      421 cgagatcctt gcgtatgacc cggcggaggc gctccgtgcc cgcttccggc gcgagggtga
      481 ccgtgcggat gtcggaagca gccagggact gcaggagcat gggggaaatc cggtccgcac
      541 gcagcgagga gagactgatc ttgtgcccgc ggccgagcag ctgttcgcaa agttgatcga
      601 gccggcggta atcggaaagg gcggcgccga ccagaccgat gcggtcaccg gggtgggcat
      661 ggcggtcaac ctccgtgatg atctcctcca gcggccagct gcggaaggga tgatagacat
      721 tgccggcagc gcaaaagcgg cagccccggc cgcagccgcg gccgacctcg accatgaaca
      781 tctcgaggtg ggtgttgggc gtgacgcaga tcgaactggc cgggggatgt tttttaatat
      841 caatatagcg cctccgcacc cttcccgact ccggctgcag gccgtgcacg gcgggcaccc
      901 acgccccctc gatacgcgcc agtcgttcca gcagctcctc ccgcccctcc ccctcatcca
      961 gatgctcctc ataggcccgg gcgaactggg ggatcatctc ctctccctcg ccgatgagaa
     1021 aaaggtcggc ggccggggcc atgacggtcg gattataaaa agcagccacc ccacccatca
     1081 ggatgagggg atcccgacgg gtgcgctccc gggccagcag ggggatgcct gcccgtttga
     1141 gcatctgcag gaccagtgga tagtccatct cgtaagcgag ggaaaaagcg atgatccgga
     1201 actggttcag gggctgctgg gattcaaggg tatgataaaa ggcagcgaac gggccttcca
     1261 gctggaaggc ccgttcgcct ttgaggagtg gctgctcgtt cagcaaccgg tagacggtct
     1321 gatatcccag actcgacata cccgttgcgt aactattggg atagacgagc gccagtggag
     1381 tggggccggt gggagagaac tcgcggcggc ggctttctgc gctgagcagc ttccgggcgt
     1441 agctccggaa ggccccgtac tgatcagcca tggcagccgc tccgtatcat ggcgcgccta
     1501 ctcgaccgcg cggctcctgc atcacaggag cttccagcgg ttgcgacggc acgcgggtat
     1561 atcgaactcg gtgtcttcat gaccattgcc gttgccggac gtaacctgga catcctgcag
     1621 cgtccgctcc acatctttcc aggtgtaagc gggctcagga cggcttgcag cgggctgctc
     1681 ctcggacctg ggctcctgct gaggcgtccg ggccggggct gccggctgcg tcgtgcgctg
     1741 ctgccatttg ggattgagga tgtcgcggct ggcctcgcga ttgacgtggt tgttgatctc
     1801 ctccagccgg gtaaccgccg ggatctcctt ctgcagcagg ccgctctgtt cgaactgggt
     1861 gctggtgcgg gtgccggcat cgcggacgcg gcggtagttg ctggaa
//
LOCUS       DAOPWU010000523         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_66904_length_1887_cov_3.716703, whole genome shotgun sequence.
ACCESSION   DAOPWU010000523 DAOPWU010000000
VERSION     DAOPWU010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66904_length_1887_cov_3.716703"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PKM23_15885"
     CDS             complement(<1..1120)
                     /locus_tag="PKM23_15885"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNY93003.1"
                     /translation="MQRDLNQLGQKEFDLLIVGGGIYGATAAREAALAGLSVALIEKN
                     DFASGTSGNSLKIVHGGLRYLQHADLKRMRESIAERRVLLRIAPHLVHPLPCVMPTYG
                     HAIKGPEVMRIAMLMNDVLSADRNLGADPGHTLPMGRVISRQRFLDLVPHVEKQRLNG
                     GAVWYDATMYNSERLALAFVRSAVEQGAVAANHLAAEEFLLREGRVTGVRARDGIGGG
                     TVEIRARMTLTCAGPWINDLFARLGERRPSEQPFSTALNLVVKRRLSEAYAFGVNSKR
                     EFKDKDALIKKGSRLLFVVPWRDYTMIGTDHRPWQGTAGEYRVSEADIVSFLREVNEA
                     MPGADIRREEVTFFYGGLLPMAGVNPGSGDVSITKHFRI"
     gene            complement(1223..>1887)
                     /locus_tag="PKM23_15890"
     CDS             complement(1223..>1887)
                     /locus_tag="PKM23_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNY93004.1"
                     /translation="KKGFINKSRRFIEGCRLPADLQHVRWMIFLQEAEKGLLYSPALG
                     EALADQDPYGFIRQAFAACDSPEPLDQQEYVDIVTYLADDIMVKVDRMSMAVSLEARA
                     PFLDYRLVEFCASLPPHLRLNGKRSKYILKRAFRGILPDEILDRRKEGFSIPIKSWMK
                     GELKPMLLDFLSPSALAATGWFEPAHVQRLIGEHLDGRENHSHRLWALLMFQMWHHQY
                     MR"
BASE COUNT          346 a          618 c          589 g          334 t
ORIGIN      
        1 ggatccggaa gtgcttggtg atgctgacat ccccgctgcc gggattgact cccgccatcg
       61 ggagcagtcc accgtagaaa aaggtcacct cctcgcggcg gatatccgcc cccggcatcg
      121 cctcgttgac ctcgcggaga aaactaacaa tatcggcctc gctcacccgg tactccccgg
      181 ccgtgccctg ccaggggcga tggtccgtgc caatcatggt atagtcccgc cagggcacca
      241 cgaagagcag gcgcgagccc tttttgatca gggcatcctt gtccttgaac tccctcttgc
      301 tgttgacgcc gaaggcgtag gcctcgctca accggcgctt gacgaccaga ttgagggcgg
      361 tcgagaaggg ctgctcggac gggcgccttt cgccaagccg ggcaaagagg tcgttgatcc
      421 aggggccggc acaggtaagg gtcatacggg cgcggatctc cacggtcccg ccgccgatcc
      481 cgtcccgggc gcgcaccccg gtcacccgcc cctcccggag gagaaactcc tccgcggcga
      541 gatggttggc cgccaccgcc ccctgctcca ccgccgagcg cacaaaggcc agggccagcc
      601 gctccgaatt gtacatggtc gcatcgtacc agaccgcccc gccgttgaga cgctgcttct
      661 cgacgtgcgg gacgaggtca aggaaacgct gccgcgaaat cacccggccc atcggcaggg
      721 tgtgccccgg atcggcgccc agattgcggt cggcgctgag cacatcattc atcagcatgg
      781 cgatacgcat cacctccggc cccttgatgg catgcccgta ggtcggcatg acgcagggca
      841 ggggatggac cagatggggg gcgatgcgca gcagaaccct gcgctcggcg atcgattcgc
      901 gcatgcgttt gaggtcggcg tgctgcaggt agcgcagtcc gccgtgcaca atcttgaggc
      961 tgttgcccga agtgcccgag gcaaaatcat tcttttcgat cagggccacc gaaaggccgg
     1021 ccagggccgc ctcgcgcgcc gcagtggctc catagatccc cccgccaacg atgagaaggt
     1081 cgaattcttt ttggccaagc tggttgaggt cgcgttgcat gatctctcct gttaaacagc
     1141 cccctcctcc ccggcgcggg actgtggtga aataaagact ccatgcggcg gattcatgct
     1201 ccgccgcccc tttgcactgg catcagcgca tgtattggtg atgccacatc tgaaacatca
     1261 gcagcgccca gagacgatgg ctgtgattct cgcgcccgtc caggtgctcc ccgatcagcc
     1321 gctgcacatg ggcgggctcg aaccagcctg tcgccgccag ggccgacggt gatagaaaat
     1381 cgagcagcat cggcttgagc tcccccttca tccagctctt gatcgggatg ctgaatccct
     1441 ccttgcgccg gtcgaggatc tcgtccggca ggatgccccg gaaggcccgc ttgaggatgt
     1501 acttgctgcg cttgccgttg aggcgcaggt gcgggggcag tgaggcgcag aactcgacca
     1561 ggcggtagtc caggaacggg gcgcgcgcct ccagcgaaac cgccatcgac atgcggtcga
     1621 ccttgaccat gatatcgtcg gccagatagg tgacgatgtc gacatactcc tgctggtcga
     1681 ggggttccgg cgaatcgcag gcggcgaaag cctgacggat gaatccatag ggatcctggt
     1741 ccgcaagcgc ctcgcccagg gccgggctgt agagcagccc cttttcagcc tcctggagaa
     1801 agatcatcca gcgcacgtgc tggagatcgg cgggcaggcg gcagccctcg atgaaacgcc
     1861 gcgacttgtt gataaacccc tttttct
//
LOCUS       DAOPWU010000524         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_67181_length_1882_cov_4.511221, whole genome shotgun sequence.
ACCESSION   DAOPWU010000524 DAOPWU010000000
VERSION     DAOPWU010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67181_length_1882_cov_4.511221"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKM23_15895"
     CDS             complement(<1..496)
                     /locus_tag="PKM23_15895"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNY93005.1"
                     /translation="MKHTLILTGLLVAAMTLQSTAFELPKPRKYTLGNGLTVYHLPQQ
                     DLPLVSFRLIIPGAGNCSTSPDQEGLADLTAELLMKGTKTRSAAEVAEASDFIGAELV
                     SRARAEYAEIGGGALSENFPALLELAAECLLQPAFSAEEVELEKGVRIDDLLAIKDEP
                     QSAVR"
     gene            complement(493..1824)
                     /locus_tag="PKM23_15900"
     CDS             complement(493..1824)
                     /locus_tag="PKM23_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011497902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNY93006.1"
                     /translation="MHLKMLPLLCMVLLGSSVCLAATFSKENITTATLDNGLKILLFE
                     DHGIPNIAYYTFYRVGSRNERPGLTGVSHFIEHMMFNGTAETGPGQLDRIMEFNGGAN
                     NAYTSDDMTAYTDWFPVAALEKMMAIEADRMQGLIFDPEVLESERGVVASERRMAVEN
                     DNASLLDETVRATAIMAHPYHWSVIGWMSDIQNWRREEIMQYYRTYYAPNNAVLVVVG
                     DFKTPEILALIKKYYGAIPASPAPAAVTTTEPEQIGPRRVTLHKPAQSPNFQMVWPAP
                     ACLDEDFPALRILEIVLLQGESSRLYRSLVSDKQLAIDIHGGMQESIDPLLFNISVDP
                     RQDAGLDLIEKAVDEELARIAAEGISEKELTKARNIIRTDFYRPLQSISGKANILGNA
                     ELLFGGWEKLFSWTGRFDEVTTAQVQQAAKKWLTPLKKTTGVLLPEEGGAQ"
BASE COUNT          316 a          516 c          648 g          402 t
ORIGIN      
        1 agcgcacggc cgactgcggt tcatccttga tcgccaaaag gtcatcgatg cggaccccct
       61 tttccagctc gacctcctcg gcggagaagg caggctggag caggcactcc gcggccagtt
      121 cgagcagggc cgggaaattc tcgctcaggg cgccgccgcc tatctcggcg tactcggcgc
      181 gggcgcggct gaccagttcg gcgccgatga aatcggaggc ctcggccact tcggcggcgg
      241 aacgggtttt ggtccccttc atgagcagct cggcggtgag gtcggccagt ccctcctggt
      301 ccgggctggt cgagcaattg cccgccccgg ggatgatcag gcggaagctg accagcggca
      361 ggtcttgctg gggcagatgg taaacggtca gtccgttgcc gagggtgtat ttccggggtt
      421 tgggaagttc aaaagcggtg ctttgcagag tcatggcggc caccagcagt ccggttagaa
      481 tcagggtatg tttcattggg cgcctccctc ttccggaagc agcactcccg tggtcttttt
      541 cagcggcgtg agccattttt ttgccgcctg ctgcacctgg gcggtggtca cctcgtcgaa
      601 ccgcccggtc caggagaaga gcttttccca gccgccgaag aggagttcgg cattgccgag
      661 gatattggcc ttgccgctga tgctttgcag cgggcggtag aaatcggtgc ggatgatatt
      721 gcgcgccttg gtcagctcct tttcgctgat gccctcggcg gcgatgcggg cgagttcctc
      781 atcaacggct ttttcgatga ggtcgaggcc ggcgtcctgg cgggggtcga cggagatatt
      841 gaagaggagg ggatcgatgc tctcctgcat gccgccgtgg atatcgatgg caagctgctt
      901 gtcgctgacg aggctgcggt agaggcggct gctctcgccc tggaggagga cgatctcgag
      961 gatgcgcagg gccggaaaat cctcatccag gcaggcgggg gcgggccaga ccatctggaa
     1021 gttgggcgac tgggccggct tgtgcagggt gacgcggcgc gggccgatct gctccggctc
     1081 ggtggtggtc accgccgccg gggcgggact ggccgggatc gcgccgtaat attttttgat
     1141 cagtgcaagg atttcggggg ttttaaaatc cccgaccacc accagcaccg cattgttggg
     1201 ggcgtagtag gtgcggtaat actgcatgat ctcctcgcgg cgccagttct ggatgtcgct
     1261 catccagcca atcaccgacc agtggtaggg atgggccata atcgcggtgg cgcgtacggt
     1321 ctcgtcgagg aggctggcgt tgtcgttctc gacggccatg cggcgttcgg aggcgaccac
     1381 gccgcgctcg gactcgagca cctcgggatc gaagatgagt ccctgcatgc ggtcggcctc
     1441 gatggccatc atcttttcga gggccgcgac gggaaaccag tcggtatagg cggtcatgtc
     1501 gtcgctggta taggcgttgt tggcgccgcc gttgaactcc atgatccggt cgagctggcc
     1561 cgggccggtt tcggccgtgc cgttgaacat catgtgctcg atgaaatggg agaccccggt
     1621 cagccccggc cgctcgttgc gcgaaccgac gcggtaaaag gtgtaatagg cgatgttggg
     1681 gatgccgtga tcctcgaaga gcaggatctt gaggccgttg tcgagtgtgg cggtggtgat
     1741 gttctccttg ctgaaggtgg ccgccaggca gaccgatgag ccgagcagga ccatgcacag
     1801 gagggggagc attttgagat gcatctctgt tctcctctgt taacaattga ttaaaatatt
     1861 aaatttatct tattttatca ag
//
LOCUS       DAOPWU010000525         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_67464_length_1877_cov_3.384193, whole genome shotgun sequence.
ACCESSION   DAOPWU010000525 DAOPWU010000000
VERSION     DAOPWU010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67464_length_1877_cov_3.384193"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="PKM23_15905"
     CDS             complement(<1..1644)
                     /locus_tag="PKM23_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HNY93007.1"
                     /translation="MMSPDHDLRADMPSRRRFLSLMSASLALAGLSSCRKPVERILPY
                     VKGVEELVPGIPVPYATTMPMGLDPTGLLVSTFDGRPTKIEGLPGHPASLGASDIYMQ
                     AAILNLYDPDRSRTVLHAGKTSDWAAFTAGWKEQLPRLQERRGEGLAVLAEASSSPTL
                     HRLQREMTGRFPLMKWVNYDSVSDENILTAARIACGVDARPLALLDHAEVILALDGDI
                     FYSESGHLRHASGFATGRRLETSEAGMNRLYVVEPGMTITGGAADHRLRLEASRIAPW
                     AHSLAAELGGLGLSSFAGFTSFATLDEASQRWLKAAARDLMAHRGRCLLIAGRRQPVE
                     LHLLLFALNLELGNLGRTLHFVPMEHALPSSLAGLKGLTEQMAAGRISHLAIFGGNPV
                     YTAPADVDFAAALAKADFSLHLSDRVDETSARTAWHLPQSHFLEEWGDAASVEGQLSP
                     LQPMIAPLFGGRSVIEMAGFLATGEERSGYELVRESWRRLLPVAGFESRWRALLHSGL
                     HNGSAPVPVAPDPGAMMAHLRNTPLPQPGESLPEICFQPS"
     gene            complement(1641..>1877)
                     /locus_tag="PKM23_15910"
     CDS             complement(1641..>1877)
                     /locus_tag="PKM23_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNY93008.1"
                     /translation="GCFSCHGNIAGMTVVTQSQPLSMGWCLGCHRHPEPNLRPAAEIT
                     SMTWQPDADHARFAAELAGRKKLNPPQDCSGCHR"
BASE COUNT          361 a          549 c          657 g          310 t
ORIGIN      
        1 ggagggctgg aaacaaattt cgggcaggct ctccccgggc tgcggcaggg gggtgttgcg
       61 gaggtgcgcc atcatcgccc ccgggtcggg cgcgaccggc acaggtgccg atccgttatg
      121 aaggccgctg tgcagcaggg cgcgccagcg gctttcaaaa cccgcgacag gaaggaggcg
      181 ccgccaactc tcgcgcacca gttcatagcc actgcgctcc tcacctgtgg ccagaaaacc
      241 ggccatctcg atgacactgc ggccgccgaa aaggggggcg atcatgggct gcagggggct
      301 caactgccct tctacgctcg cggcgtcgcc ccattcctcg aggaaatgac tctgcggcag
      361 atgccacgcg gttcgcgcgg aggtttcgtc gacccggtca ctgaggtgaa gggagaaatc
      421 agcctttgcc agggcggcgg cgaaatcgac atcggcaggg gcggtataga ccggattgcc
      481 gccgaatatg gccagatggc tgatccggcc ggcagccatt tgctcggtga gccccttcaa
      541 tcccgccagg ctggagggaa gggcatgttc catcggaacg aagtggaggg tacggccgag
      601 gttgccgagc tcaagattga gggcgaaaag caggagatgc agctcgaccg gctgccggcg
      661 gccggcgatc aggagacagc ggccgcgatg ggccataaga tcgcgcgccg ctgctttgag
      721 ccagcgctgg ctggcttcgt ccagcgtagc aaaggaggtg aaaccggcaa aggaggagag
      781 accgagacct cccagttccg ccgccagcga atgggcccat ggtgcgatgc gggaggcctc
      841 gagacgcagc cggtgatccg cagcgccgcc ggtgatggtc attccgggtt cgaccacata
      901 gagccggttc atccctgctt cggaggtctc gagcctgcgt cctgtcgcga aaccgctagc
      961 gtggcggaga tggccgctct cgctataaaa aatatctcca tccagtgcga ggatgacctc
     1021 cgcatggtca agcagggcga gggggcgggc atcaaccccg caggcgatgc gggcggcggt
     1081 gagaatgttt tcatcgctga ctgaatcgta attaacccac ttcatgagcg gaaagcgccc
     1141 ggtcatctcc ctctgcaggc ggtggagggt cggtgatgag gaggcttcgg ccaggaccgc
     1201 caacccctcg ccgcggcgct cctgcagccg gggcagctgc tctttccagc cggccgtaaa
     1261 ggcggcccag tccgatgttt ttccagcatg caggaccgta cgcgagcggt cgggatcata
     1321 gagattgagg atggcggcct gcatatagat gtctgaagcc ccaagcgagg caggatggcc
     1381 aggcaagccc tcgatcttcg tcggccggcc gtcaaaagtg gagacgagca ggcccgtcgg
     1441 atcgagtccc atgggcatgg tggttgcgta gggcaccggg atgccgggga cgagttcctc
     1501 gacccccttg acgtagggaa ggattctctc gacgggttta cggcaggagg agaggccggc
     1561 gagggcgagc gatgcgctca tcagggagag aaaacggcgg cggctgggca tatccgcacg
     1621 caagtcgtga tctgggctca tcatcggtgg catcccgaac aatcttgagg cggattcagt
     1681 tttttccggc cggccagttc ggcagcgaag cgggcatgat cagcatcggg ctgccaggtc
     1741 atggaggtga tctcggcggc aggccggagg ttcggctcgg ggtggcgatg gcagccgagg
     1801 caccacccca tgctcagagg ctgactctgt gtcaccaccg tcataccggc gatgttgcca
     1861 tggcagctga aacatcc
//
LOCUS       DAOPWU010000526         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_67508_length_1876_cov_4.428885, whole genome shotgun sequence.
ACCESSION   DAOPWU010000526 DAOPWU010000000
VERSION     DAOPWU010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67508_length_1876_cov_4.428885"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..>1876)
                     /locus_tag="PKM23_15915"
     CDS             complement(262..>1876)
                     /locus_tag="PKM23_15915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNY93009.1"
                     /translation="TPPARETETPEEGFFICDRRGRFLSANRSLQTMLNYAEEELLEL
                     SLSDLLSREEEQRLFNDIFTCSPDQEIFPIQLHFIDKVGERRPVSLKIRMLRDESREK
                     RVIGFRGSVTLVAAERGMPAGVHGMLDQGVIIRHLFDLVQMSYTEPLNVLLKRIGEVV
                     CQIFGFQRSTIALLDRRKKAYIKHAMVGYGENRTVAAGEKTLEVPEEVVDRLFVDKAQ
                     VKVIYYSQESGSLGSPALPLTQTELPTVESRWHPRDLALLRLADHRGHQFGYISIDEP
                     LDNVTPARSTFDNLELFSQLVAMVIENYYRFSTIERRNRRLKQILINSNIFKLYLSLN
                     ELLKEVVWSVKFSLEFNLVSLVLISKKSGQLETKAVACDDRIKLGQLQELAFDLRDFS
                     ALLREEYRRGKSYLISSEEEVLRHFKQIYYGSLSNGHYADGWPRYALLLVPIKSREGK
                     IIGFILADDPADGKLPTTESVHIMEILANQVAIAIDNRMLYVQAKEKPQSTEPPAASE
                     PADGPDAGVTDYPEENSTGLRRLVDRFLR"
BASE COUNT          387 a          497 c          605 g          387 t
ORIGIN      
        1 gcgctgccga ggaccttgag gtcgggctgg cgcgggtcga ccccggcggc ggccgaaacg
       61 acgcgggcct ggcccggagt tgaggctgcg gcggcctggc ggaggttggg aagaaggcag
      121 gtgccgagtc cgatcatgct gccggtatga agaaactggc gtctgttcat aggcttccgc
      181 cgggtgaaat aaaaaaccgt gatcccctcg cagaccgcac gagatcacgg ttgaactttc
      241 gcaaatgcgg tcggctgatg gtcagcgcag gaaacggtcc acgagcctgc gcagtccggt
      301 gctgttctct tcaggatagt ccgtcacgcc ggcatcgggt ccatccgcag gctccgacgc
      361 agcgggcggt tcggtgctct gaggtttctc cttggcctga acatagagca tccggttatc
      421 gatggcaata gcaacctggt tggcgagaat ctccatgata tggacgcttt cagtggtcgg
      481 cagtttcccg tccgccggat cgtctgcgag gataaagccg atgatcttgc cttcgcggga
      541 cttgatgggc acaagcagca gggcatagcg cggccatccg tccgcatagt gcccattgct
      601 gaggctgccg taatagatct gcttgaagtg gcgcagcacc tcctcctcac tgctgatgag
      661 gtaagatttg ccgcggcggt actcctcgcg caggagcgca ctaaagtctc gcaaatcgaa
      721 ggcaagttcc tgaagctggc cgagcttgat gcggtcatcg caggcgacgg ccttggtttc
      781 aagctgtccg ctctttttgc tgatgagaac gagggaaacc agattgaact cgagagaaaa
      841 tttgacggac cagaccacct ctttgagcag ctcgttgagg cttaggtaga gcttgaagat
      901 attgctgttg atgaggatct gtttcagccg gcggttgcgg cgctcgatgg tggagaagcg
      961 gtaatagttc tcaatcacca tcgctaccag ctgactgaag agctcgagat tatcaaaggt
     1021 ggagcgggcc ggagtgacgt tatcgagggg ctcgtcgatg gaaatatagc cgaactggtg
     1081 gccgcggtgg tcagcgaggc ggagaagggc cagatcgcgc ggatgccagc gcgattcaac
     1141 ggttggaagt tccgtctggg tgagaggcaa ggccgggctg ccaagagatc cgctctcctg
     1201 gctgtaatag atgaccttta cctgggcctt gtcgacaaaa aggcggtcaa cgacctcctc
     1261 gggcacctcg agagtctttt cccccgccgc aacggtgcga ttttcgccat agccgaccat
     1321 ggcgtgcttg atgtaggcct ttttgcgccg gtcgagcagt gcgatggtgg agcgctgaaa
     1381 accgaaaatc tggcatacca cttcgccaat gcgcttgagc aggacattga gaggttcggt
     1441 gtagctcatc tgcaccaggt cgaagaggtg acggattata acgccttggt cgagcatccc
     1501 gtgcacaccg gccggcatcc cccgttcagc ggcgacaaga gtgacgctgc cgcgaaaacc
     1561 gataacgcgt ttttccctgg attcatcccg aagcatgcgg atcttcaggc tgaccggccg
     1621 cctctccccc accttgtcga tgaaatgcag ctggatggga aagatttcct gatcagggct
     1681 gcaggtgaag atgtcgttga agaggcgctg ctcctcctca cgggagagca ggtcggagag
     1741 ggaaagttcg agaagctcct cttcggcata attgagcatg gtctgcaggg acctgttggc
     1801 agagaggaag cgtccacggc ggtcgcaaat gaaaaagccc tcctcggggg tttcagtctc
     1861 ccgggcagga ggtgtg
//
LOCUS       DAOPWU010000527         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_68556_length_1858_cov_5.610649, whole genome shotgun sequence.
ACCESSION   DAOPWU010000527 DAOPWU010000000
VERSION     DAOPWU010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68556_length_1858_cov_5.610649"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PKM23_15920"
     CDS             <1..599
                     /locus_tag="PKM23_15920"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNY93010.1"
                     /translation="VLVRPGPGVPVGAEQDPDPANHSKPILGANDGASGVAVLLEIAR
                     LIRQKPPRCGVDIVLFDAEDQGLEGAESSYARGSQHFARTVVPTFRPRFGILLDLVGD
                     ANLNIYQEHYSLNYARPVVEKVWNAAAALGVTEFIPQPGYAVYDDHVPLLQRGIPCID
                     IIDFDYPPWHTLADTPDKCSPASLEKVGRVVLQVLYSE"
     gene            647..1501
                     /gene="prmA"
                     /locus_tag="PKM23_15925"
     CDS             647..1501
                     /gene="prmA"
                     /locus_tag="PKM23_15925"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNY93011.1"
                     /translation="MSTQEIYFAVTVPSTPECGDALASYLFDLGAAGVVENEGSLTAS
                     FKGARHELLTPIRSYLAELRALGYQVEPEAVVCEEIVNRDWNAEWKKSYHGFAVSEKI
                     FIKPTWEAPPPVAPPCLIEIDPEMAFGTGTHATTRLCLQLLEERLYPGCRVLDIGTGT
                     GILAIAAAKLGAGAIAAFDIDPVAAETAGRNAAANGVRDRMEIFAGQLADLTPRPFDL
                     ILANIQRLEIVGMLALLRQLFPAPAEFIFSGILRDEEELFRATLAADHFRVNDMRYEE
                     EWLACLAG"
BASE COUNT          338 a          591 c          567 g          362 t
ORIGIN      
        1 gggtcctggt ccgcccaggg ccgggtgtcc cagtgggcgc agagcaggat ccggatccgg
       61 ccaaccacag caagcccatc ctcggcgcca atgacggtgc ttccggggtg gccgtgctgt
      121 tggagatcgc ccgcctcatc cgtcagaaac ctccccgctg cggggtcgac atcgtccttt
      181 ttgatgcgga ggatcagggg ctggagggag ccgaatcgag ctatgccagg ggctcgcagc
      241 atttcgcccg cacggtcgtt cccaccttcc ggccgcgctt cggcatcctg ctggatctgg
      301 ttggcgatgc caacctcaat atctaccagg agcattattc gctcaactac gcccggcccg
      361 tggtcgaaaa ggtctggaac gccgccgccg cgctcggggt gaccgagttc atcccccagc
      421 cgggctatgc ggtctatgac gaccatgtgc ccctgctgca gcgcggcatc ccctgcatag
      481 atatcattga tttcgattat ccgccctggc acaccctggc cgatacgccc gataaatgca
      541 gtccggcctc gcttgaaaaa gtggggcggg tggtgttgca ggtgctctat tcggaatgaa
      601 gcggccgccg ggccgccgtc ctgaccgact gaaaaggaat cccgccatgt ccacccagga
      661 gatctatttc gccgtcaccg tccccagcac gccggagtgc ggcgatgccc tcgccagcta
      721 tctcttcgac ctcggcgccg ctggggtggt tgagaacgag ggcagcctga ccgcctcctt
      781 caagggggcg cggcacgagc tgctcacccc catccgcagc tacctggcgg agctgcgggc
      841 cctgggctac caagtcgaac ccgaagcggt ggtctgtgag gagatcgtca accgcgactg
      901 gaacgccgag tggaaaaaaa gctaccacgg cttcgccgtc agtgaaaaaa tcttcatcaa
      961 gccgacctgg gaggcgcctc cccccgtggc acccccctgt ctgatcgaga tcgatcccga
     1021 gatggccttc ggcaccggca cccacgccac cacccgactc tgtctgcagc tgctcgagga
     1081 gcgcctttac cccggctgcc gcgtgctcga tatcggcacc ggcacgggca ttctcgcgat
     1141 tgcggcggcc aaactgggcg ccggcgccat cgccgccttt gacatcgatc cggtcgcagc
     1201 cgagacggcg ggacgcaacg ccgccgccaa cggggtacgc gaccgcatgg agatctttgc
     1261 cgggcagctt gccgacctga ccccccggcc cttcgatctc atcctggcca acatccaacg
     1321 cctcgagatc gtcgggatgc tcgccctgct gcggcagctc tttcctgcac cggccgaatt
     1381 tatcttttcc ggcatcctcc gcgacgagga ggagctcttc cgcgccaccc tggccgcgga
     1441 ccatttcaga gtgaacgata tgcgttatga ggaggagtgg ttggcctgcc tggcggggta
     1501 ggccttttat tgcaaaattg tgatccggca caggatcatt tatggtttaa ttgcgttaaa
     1561 ttaggtgtca tgagggagag ggaaccctgc gtccggttcc cttttttatt gaaagcggtg
     1621 aaatggaatg ccctgctgcg ggtgttatag actttgtttc cggttccgct gtcctgttga
     1681 acaaaatgag gaagatgaag atgaaaagaa aattacagtt cgggattttt accctcggcc
     1741 tgcttctggc tgccttttcc gccacagcgg cggaaagaaa gcaggaggct gtaccggccc
     1801 tgaaggcggc gcagacctcg gcagcgctgc aggaggcggc ggaggtgatg gtcaaggc
//
LOCUS       DAOPWU010000528         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_69481_length_1842_cov_4.697258, whole genome shotgun sequence.
ACCESSION   DAOPWU010000528 DAOPWU010000000
VERSION     DAOPWU010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69481_length_1842_cov_4.697258"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /gene="hycE"
                     /locus_tag="PKM23_15930"
     CDS             complement(<1..358)
                     /gene="hycE"
                     /locus_tag="PKM23_15930"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="formate hydrogenlyase subunit 5; HycBCDEFG is part
                     of the formate hydrogenlyase system which is involved in
                     the cleaving of formate to dihydrogen and carbon dioxide;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HNY93012.1"
                     /translation="MHSTAYCQAVEKAAEIEVPARGRFIRVICLELERIQSHLLWLGI
                     AGHIIGFDTVLMQAWRIREPVMWLCEKITGNRKLLGMNLVGGVRRDVPRGIHDELLEV
                     LARVEKESTAVLAAIAG"
     gene            complement(365..1075)
                     /locus_tag="PKM23_15935"
     CDS             complement(365..1075)
                     /locus_tag="PKM23_15935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNY93013.1"
                     /translation="MTVQEMANHLGKEFGARIRHSEIPVPDMLLLHIDRSDSVAFNRY
                     LFHDLSGRFVVAVGTDYRSETGGYLVDYVYSFAEHHLFITLRLSLPAKDLWIEAITGS
                     ADIPAANWAEREIQDMLGIPLRNHPDPRRLMLADDWPADLHPLRRDMPLQTYPDKAAG
                     MTPVFKEPPEGATVVPIGPFFPVLEEPAQFRLFIEGERVVGGDYRGFYNHRGVEKIAD
                     SQLNYNQVPFLAERICGI"
     gene            complement(1098..>1842)
                     /locus_tag="PKM23_15940"
     CDS             complement(1098..>1842)
                     /locus_tag="PKM23_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93014.1"
                     /translation="ISAVTLASFVKFFGALFFGKYEDNGSKARAGDVPAAMRLPQIIL
                     AALCILFGLLPQLPLGWVYRSIAYLFPPGSMPAAASIYGGGLLNGLSLNVAGSTAAWN
                     ALLLTVIFGLCALLAWGLFRAGGARVRSVETWYCGEEMSDRDTRYRAHGFYLPFKQIF
                     RLRIGRYERPGIYPTITWPKRLFRKEPALNRAIDLDRWLYQPLARSAVRLMRRFSATH
                     SGVPHLYLLWLAAGAALALIILYSFSGGK"
BASE COUNT          366 a          557 c          608 g          311 t
ORIGIN      
        1 cgccggcgat ggccgccagg acggcggtgc tctccttttc gacccgggcg agcacctcga
       61 ggagctcgtc atgaatcccc ctgggcacgt cgcggcggac tccgccaacc aggttcatgc
      121 ccagcagctt gcggttgccg gtgatctttt cacaaagcca catcaccggc tcccgaatgc
      181 gccaggcctg catcagcacc gtatcgaagc cgatgatatg gccggcgatg ccgagccaga
      241 gcagatggct ctggatacgc tccagctcca gacagatgac gcggatgaag cggccgcggg
      301 ccgggacctc aatttctgct gctttctcta ctgcttgaca gtacgccgta ctgtgcaccg
      361 accctcatat gccgcagatc cgctccgcga ggaacggcac ctggttatag ttgagctggc
      421 tgtcggcgat cttttcaacc ccgcgatggt tgtaaaagcc gcggtaatcc ccgccgacca
      481 ctctctcgcc ctcaatgaag aggcgaaact gggccggttc ctccagcaca gggaaaaaag
      541 gtccgatcgg caccaccgtc gccccctcag gcggctcctt gaagacaggc gtcatccccg
      601 cggccttgtc cgggtaggtc tgcaggggca tatcccgtcg aaggggatga aggtccgccg
      661 gccagtcgtc ggcgagcatg agccggcgtg ggtcgggatg gttgcgcagg gggatgccga
      721 gcatgtcctg gatctcgcgc tccgcccagt tggccgccgg tatatccgcc gagccggtga
      781 tggcctcgat ccagagatcc ttggccggca gactcagcct cagagtgatg aagaggtggt
      841 gctctgcgaa agagtagacg taatcgacca ggtagccgcc ggtttcgctg cgatagtcgg
      901 tgccgacagc gacgacaaaa cggccggaga gatcatggaa aaggtagcgg ttgaatgcga
      961 cactgtcgct gcggtcgatg tggagcagga gcatatcggg gaccgggatc tcgctgtggc
     1021 ggatgcgggc gccgaattcc ttgccgagat ggttcgccat ctcctgtaca gtcatggcca
     1081 agcttgcctc ctctctgcta cttgccgccg gaaaaggaat agaggataat cagggccagc
     1141 gccgctccgg ccgccagcca taacagatat agatggggaa cgccgctgtg ggtggcgctg
     1201 aagcgccgca tcagcctgac cgccgagcgc gccagcggct ggtagagcca gcgatcgagg
     1261 tcgatggccc ggttgagggc cggttccttt ctaaagagcc ttttcggcca ggtgatggtc
     1321 ggataaatgc cgggccgttc ataacggccg atgcggagcc ggaagatctg cttgaagggg
     1381 agataaaagc catgggctcg gtagcgcgta tcccggtcgc tcatctcctc cccgcagtac
     1441 caggtttcga cggagcgcac ccgggcgccg ccggcgcgga agagccccca ggccagcagg
     1501 gcgcaaaggc caaagatgac ggtcaggagg agggcgttcc atgcggccgt gctgccggcg
     1561 acgttcaggg agaggccatt caggaggccg ccgccataga tcgaggccgc cgccggcatc
     1621 gatcccggcg ggaaaagata ggcgatgctg cggtaaaccc accccagcgg cagctgcggc
     1681 aaaaggccga agaggatgca gagagcggcg aggatgatct gcggcaggcg catggctgcc
     1741 ggtacatccc ccgccctggc cttgctgccg ttatcctcgt atttgccgaa aaaaagcgcg
     1801 ccgaagaatt tcacaaaaga ggcaagggtg acggccgaga tg
//
LOCUS       DAOPWU010000529         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_69510_length_1842_cov_2.869614, whole genome shotgun sequence.
ACCESSION   DAOPWU010000529 DAOPWU010000000
VERSION     DAOPWU010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69510_length_1842_cov_2.869614"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="PKM23_15945"
     CDS             complement(<1..1400)
                     /locus_tag="PKM23_15945"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNY93015.1"
                     /translation="MKSRALKTILALALLAGVPPLSAQMIAEITSPVETEFGTYLPQP
                     SLILPAAEGYSVAPDLSNVVNAGDFSFDAAERDLLARNHFFVTPKRSGDHSGYKEMYD
                     LYNEAREQNIPIFVTTDALLHSFHLIFDHMLMEIEEAHFYGDLDRLLSGLLEIVQYQQ
                     YPAVNEDSTRAAIHQAMDYLIVAKCMLDSTFDPKINGGSYIPECSLIRSHNGPAASPI
                     FHYEEDYTQYIVRGHYTRSDSLARYFRAMMWLGRMTFAADPDPLFAPLNHRATLSALL
                     LLQAMERLTINGEPALTVWERIYAPTVFFVGKSDDTTPLAYLELIRQIYGDDFRSLDV
                     NQLGAEPRFSSFLAAAMDLPGPLIEYPGQPKGFRLMGQRFIPDSYVLDQLVFNNIPDR
                     FMPTGLDVMAVLGSGRAYSLLKEQGEMANAAYKQRLDELRTEFKAYPAETWAQNLYWN
                     WLYSLMPLLWAKGAGYP"
     gene            1594..>1842
                     /locus_tag="PKM23_15950"
     CDS             1594..>1842
                     /locus_tag="PKM23_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNY93016.1"
                     /translation="MSRLEIIGLIVSYVYAFSLMTIVEAAGKRLRWPQDLSRKIIHIG
                     AGMWVWGILWFFDELRWGIIPFATFILLNYIFYKRQTFS"
BASE COUNT          349 a          485 c          646 g          362 t
ORIGIN      
        1 ggatagcccg cccccttggc ccagagcagg ggcatgaggc tgtagagcca gttccagtag
       61 agattctgcg cccaggtttc cgccggatag gccttgaact cggtgcgcag ctcgtcgagc
      121 cgctgtttat aggctgcgtt ggccatctcg ccctgctctt taagaaggct gtaggcgcgt
      181 ccggagccga gcacggccat gacgtcgagg ccggtgggca tgaagcggtc tgggatgttg
      241 ttgaaaacga gctggtcgag gacgtagctg tcggggatga agcgctgtcc catgaggcgg
      301 aagcccttgg gctggccggg atactcgatg aggggtcccg gcaagtccat cgccgcagcc
      361 agaaaggagc tgaagcgggg ctcggcgccg agctggttga catcgaggct gcgaaagtca
      421 tcgccataga tctggcggat cagctccagg taggcgaggg gggtggtgtc gtccgacttg
      481 ccgacaaaaa agacggtggg ggcgtagatg cgctcccaga cggtgagggc gggctcgccg
      541 ttgatggtga gccgctccat ggcttgcagc aggaggaggg cgctgagggt ggcgcggtgg
      601 ttgaggggcg cgaagagggg gtccggatcg gcggcgaagg tcatgcgccc gagccacatc
      661 atggcgcgga aatagcgggc gagagagtcg ctgcgggtgt aatggccgcg gacgatgtac
      721 tgggtgtaat cctcctcata gtgaaagatg ggcgaggcgg ccggcccgtt gtggctgcgg
      781 atgaggctgc actcgggaat atagctgccg ccgttgattt tcgggtcaaa ggtggaatca
      841 agcatgcatt tggcgacgat gagataatcc atggcctgat ggatggcggc gcgggtcgag
      901 tcctcgttca cagcggggta ctgctggtac tggacgatct cgaggagtcc gctgaggagc
      961 cggtcgaggt cgccgtaaaa atgggcctcc tcgatctcca tgagcatgtg gtcgaagatg
     1021 aggtggaagg agtggaggag ggcgtcggtg gtgacgaaga tggggatatt ctgctcgcgc
     1081 gcctcgttgt agaggtcgta catctccttg tagccgctgt ggtcgccgct gcgtttgggg
     1141 gtgacgaaaa aatggttgcg cgcgaggagg tcgcgctcgg cagcgtcaaa gctgaaatcg
     1201 ccggcgttga ccacattgct gaggtcggga gcgacgctgt agccctccgc cgccggcaga
     1261 atgagcgagg gctgcgggag ataggtgccg aattcggtct cgaccgggct ggtgatctcg
     1321 gcgatcatct gtgcagagag gggcgggacg ccggccagaa gcgccagcgc cagaatggtt
     1381 ttcaacgcgc gggatttcat gacgacctcc aggcccgctg cgggcccgct aatgctctcc
     1441 cccttatttg aaatatgcac aacggggggc ataaattcaa gcagattttt tccgccgggg
     1501 tgcaataaac cagcctcgaa tgggagttac atatgcaaaa ggccggcccc cccggatatg
     1561 ctgcgctaat ccctgactgc gggagtatga cccatgtcca gactcgaaat catcggtttg
     1621 atcgtcagct acgtctacgc cttttccctc atgaccatcg tcgaggcggc aggcaagcgc
     1681 ctgcgctggc cgcaggacct cagccgcaag atcatccata tcggcgccgg gatgtgggtc
     1741 tggggcatcc tctggttctt cgacgagctg cgctggggca tcatcccctt cgccactttc
     1801 atcctgctca attatatctt ttacaaacgc cagaccttct cc
//
LOCUS       DAOPWU010000530         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_69563_length_1841_cov_3.539754, whole genome shotgun sequence.
ACCESSION   DAOPWU010000530 DAOPWU010000000
VERSION     DAOPWU010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69563_length_1841_cov_3.539754"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="PKM23_15955"
     CDS             complement(<1..1426)
                     /locus_tag="PKM23_15955"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNY93017.1"
                     /translation="MRREGFLSEAQLRAELNRCEYCEEKPCREACPAHCSPADFIMAA
                     RTGELADYRRAAALILGANPLGGVCGLVCPDSFCMAACVHAGFSTPIEIPAVQATLIH
                     KTHECGLPPFDKPLPNGKRIAVIGAGPAGIGAAAVLAQLGYAIDLYEQKQVLGGMARL
                     IPDFRLNKKVLQADIDFFLSLGEIALHRKHIDDPATLLVSCAAVIVCCGLERPIALEV
                     PGGEAALSWQRFLESRTRLQGERVAVIGGGAVAVDVAVSARRRGAGHVELIYRRKAEN
                     MPLTAYERELILREGIELTTCARVLEVVLHDVRVAGLRIEKLLLPPGASPEPRNFIPD
                     PQEPSHFRRFDRVIAAIGSRSAAPLREFPGLFYAGDLVLGSSTVVESVASGKNAALAA
                     DAFVRGQKPPVINKPAKSRAILSGRNLRPVALDCDFFGRRILSPFLISAAPHSDGLLQ
                     MRTAYEKGWAGAVVKTAFDNVPVHI"
     gene            complement(1521..>1841)
                     /locus_tag="PKM23_15960"
     CDS             complement(1521..>1841)
                     /locus_tag="PKM23_15960"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNY93018.1"
                     /translation="QSLYGIDFAMTRRELFESYSSEPNHNMVFTGVDIIDGRITKWLV
                     ENSWGEARGKQGYLHMTDDWFDLYVQEIVIDKKYIPAEMLKIFDSKATLLPPWDPMAE
                     RVGL"
BASE COUNT          328 a          563 c          627 g          323 t
ORIGIN      
        1 ggatgtggac cggaacgttg tcgaaggcgg tcttgaccac cgccccggcc cagccctttt
       61 cataggcagt gcgcatctgc agcagtccgt ccgagtgcgg agccgcggag atgagaaagg
      121 gggagaggat gcggcggccg aaaaagtcgc agtcgagcgc caccggccgc aggttccttc
      181 cggacaggat ggcgcggctt ttggcaggct tgttaatcac cgggggcttt tggccgcgga
      241 caaaggcatc cgctgcgagg gcggcgttct tgcccgaggc caccgactcg accacagtcg
      301 aggagcccag gacgagatcg ccggcataaa agaggccggg gaattcacgc aatggcgccg
      361 cgctgcggct gccgatagcg gcgatgaccc gatcgaagcg gcggaagtgg gagggctctt
      421 gcgggtcggg gatgaagttg cgcggctcgg gggaggcgcc cggcgggaga aggagctttt
      481 cgatgcgcag gccggcgacc cgaacgtcgt gcagcaccac ctccaggacc cgcgcgcagg
      541 tggttagttc gatgccctcg cgcaggatca gctcgcgctc gtaggcggtg agcggcatgt
      601 tctcggcctt gcggcggtag atgagttcga cgtggccggc gccgcggcgg cgggcgctga
      661 cggcgacatc gacagccacg gcccccccgc cgatcacagc cacccgctcg ccctgcaggc
      721 gggtgcggct ctcgagaaac ctctgccagc tgagggcggc ctcacccccc gggacctcga
      781 gagcgatcgg gcgctcgagt ccgcagcaga cgatcaccgc tgcgcaggaa accaggagtg
      841 tggccgggtc gtctatatgt ttgcgatgga gggcgatctc cccgagggag aggaaaaaat
      901 cgatgtccgc ctgcaggacc ttcttgttaa gacgaaagtc tggaatcagc cgggccatgc
      961 cgccaaggac ctgcttttgt tcgtagaggt cgatggcgta gcccagctgc gccagcaccg
     1021 ctgccgcccc gatccccgcc ggtccggcgc cgatgacggc gatgcgtttt ccgttgggga
     1081 ggggcttgtc gaacgggggg aggccgcact catgcgtctt gtggatcagg gtcgcctgca
     1141 cggccgggat ctcgatgggc gtcgaaaagc cggcatggac gcaggccgcc atgcaaaagc
     1201 tgtccgggca gaccaggccg cagaccccgc ccaggggatt ggccccgagg ataagggcgg
     1261 cggcgcggcg gtaatcagct agctcgccgg tgcgcgctgc catgatgaaa tcggccggcg
     1321 agcagtgggc cggacaggcc tcacggcagg gcttttcttc gcagtattcg caccggttca
     1381 gctcggccct gagttgggcc tcgctgagga atccctctct tctcatgcag cagctcccgt
     1441 acggtccgcc ggttcgtgcg caagcgccgc gtttgcacac ccgaccctcg cattttatag
     1501 tcatccccac aagccaaatc ttaaagtccg actctttcgg ccatcggatc ccagggcgga
     1561 agcagggtcg ccttcgagtc gaaaatcttg agcatttccg cggggatata ttttttgtcg
     1621 attacgatct cctgcacata gaggtcaaac cagtcgtcgg tcatatgcag atagccctgc
     1681 ttgccgcggg cttcccccca ggaattttcc accagccatt tggtgatgcg gccgtcgatg
     1741 atgtcgacgc cggtgaagac catgttgtgg ttcggctcac tgctgtagct ctcaaagagc
     1801 tcccggcgcg tcatggcgaa atcaatgccg tagagggatt g
//
LOCUS       DAOPWU010000531         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_70888_length_1819_cov_3.154195, whole genome shotgun sequence.
ACCESSION   DAOPWU010000531 DAOPWU010000000
VERSION     DAOPWU010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70888_length_1819_cov_3.154195"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKM23_15965"
     CDS             <1..216
                     /locus_tag="PKM23_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93019.1"
                     /translation="ASGDVHSIEIVYTAYSQGGIIQVTKGSPDTMQLEVIQTVPAIPF
                     TPRTPATGFGSDAALGVLNIQKYVRVK"
     gene            352..>1819
                     /locus_tag="PKM23_15970"
     CDS             352..>1819
                     /locus_tag="PKM23_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93020.1"
                     /translation="MKKLKLVLLCLIVSSLSLQAQVPEPFPEKEEKPDKKEITVIGYY
                     FMRGEQSNTAPTNEFLKGQVVGRLFGGNTVQTSSVRSSFFEQRFIPMIAYSPRLFDGW
                     AMVRMNFELDWTLGDANYGAGGNLGGAFAADFVNLQTQNLFVEFRPKKTFFVNAGMTR
                     LFDNVAVPAYTGTSHLINTGYRLAFWGSDASGIAMHNLWPNQRIKAGAYQLYENNVNE
                     DDDVTLFEVDYERDLDIVNSIGVSFWHLRDHGNGEGGVSILGQGLNCTLSNYNGVFNF
                     DFGDEDYTAGINWLGTHFHGNPLLNQGRFGYSGFGIMNFGQAKTATRTVDILGFGANL
                     RVAYKYGRTPNDFVAADGIFTTGDKKNIHDGKYSGVLTGNNWSSPGAVFYGHGLYILL
                     PHGLVVNRFTAAVVDIQNVGYGFAGGSLTGSYDIIRNKFKAKIGAGAAMAPVAPSQGG
                     NFIGAEANLNLCYTPRVFMDLELHAAYMKLGDFFDSPEM"
BASE COUNT          375 a          629 c          457 g          358 t
ORIGIN      
        1 gcctctggcg atgtccactc catcgagatc gtctacaccg cttattcgca gggcggcatc
       61 atccaggtca ccaagggcag cccggatacc atgcagctcg aggtcatcca gaccgttccg
      121 gccatcccct tcaccccgcg caccccggcc accggcttcg gctccgacgc cgccctgggc
      181 gtcctgaaca tccagaaata cgtgcgcgta aagtaatcgt tctctgtgta atttcgcccg
      241 ccttgcggat cccaactcca ggggtccgca aggctcttgt tcttcactac tgcaacattt
      301 tcacgatctc cccaaccggc tgttggtgtc acctaaaaag tgagcggatg catgaaaaaa
      361 ttaaaacttg ttctactctg cctcatcgtc agcagcctct cgctgcaggc ccaggttccg
      421 gagccctttc ctgaaaagga ggaaaaaccg gacaaaaaag agatcaccgt gatcggctat
      481 tacttcatgc gcggagagca gagcaacacg gcgccgacca atgaatttct caaaggccag
      541 gtcgtgggac gcctcttcgg cggcaatacc gtccagacct ccagtgtgcg ctcgagcttt
      601 ttcgagcagc gcttcatccc gatgatcgcc tacagcccgc gcctcttcga cggctgggcg
      661 atggtgcgca tgaatttcga actcgactgg accctcggcg atgccaacta cggcgccggc
      721 ggcaacctcg gcggggcctt cgccgccgat ttcgtcaacc tccagaccca gaatctcttc
      781 gtcgagttcc gtcccaaaaa aacctttttc gtcaacgccg gcatgacccg gctcttcgat
      841 aacgtcgctg tgcccgcata caccggcacc agccacctca tcaacaccgg ctaccgcctc
      901 gccttctggg gctccgatgc cagcggcatc gccatgcaca acctctggcc caatcagcgc
      961 atcaaggccg gggcctatca gctctatgag aacaatgtca atgaagacga tgacgtcacc
     1021 ctcttcgagg tcgactatga gcgcgatctc gatatcgtca attccatcgg cgtctctttc
     1081 tggcatctgc gcgaccatgg caatggcgaa ggcggggtct ccatcctcgg ccaggggctc
     1141 aactgcaccc tgagcaacta caacggcgtc ttcaattttg acttcggcga cgaggattat
     1201 accgccggca ttaactggct gggaacccat ttccacggca accccctgct caaccaggga
     1261 cgcttcggct acagcggctt cggcatcatg aacttcggcc aggccaagac cgccacaaga
     1321 accgtcgaca tcctcggctt cggcgccaac ctgcgcgtgg cctacaagta cggccgcacc
     1381 cctaacgatt tcgtcgccgc cgacggcatc tttaccaccg gcgataaaaa gaacatccac
     1441 gacggcaagt acagcggcgt cctcaccggc aacaactggt cctcgcccgg tgcggttttc
     1501 tacggccacg gcctctacat cctcctgccc cacggcctgg tcgtcaaccg cttcaccgcc
     1561 gcggtggtcg acatccagaa tgtcggctac ggctttgccg gcggctccct caccggctcc
     1621 tatgacatca tccgcaacaa attcaaggcc aagatcggcg ccggcgccgc catggccccg
     1681 gtcgcgccca gccagggcgg caactttatc ggcgcggagg ccaacctaaa cctctgctac
     1741 acgccccggg tcttcatgga tctcgagctg cacgcggcct atatgaagct gggtgatttc
     1801 ttcgattcac cggaaatgt
//
LOCUS       DAOPWU010000532         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_71001_length_1817_cov_4.712826, whole genome shotgun sequence.
ACCESSION   DAOPWU010000532 DAOPWU010000000
VERSION     DAOPWU010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71001_length_1817_cov_4.712826"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="PKM23_15975"
     CDS             complement(<1..1591)
                     /locus_tag="PKM23_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNY93021.1"
                     /translation="MMWMIGVVVFTLVSSAPGGERSAGLDPLLQLKLLKGRSGSEAGL
                     GKAGSAESITVIASGQFSAEALVERGARVRWQRGDLAILTVEPGLLEEVAGTPGLLYL
                     EAGVKSRPVADVSIPDVGGIAARNASGLAGRGVLIGIIDSGIDWRHQDFITPEGESRI
                     RAILDLSEPGAYYGGTMVTAAQIKAALTAGTELPTHDYSGHGTHVAGIAAGDGSSGAG
                     LGSYAGMAPEAGLVIVKASRDPFVSEFSSEDQMIAIGFIDSVATAAGMPCIINLSFGT
                     TFGAHDGTAAVERYIDALSGPGRIFVAAAGNEADKKHHALVNPSSGTAGISFTIPAYT
                     PAAGSGNDYLLLDGWYDGGSQAAVTLITPTDETIGPVGYGRYYSGNTRSGEVNIWNGF
                     YESGEEIVSGPNPFNGDRECIIEINDGAGTAPAAGEWKLRFSGSSERLDMWIASGTMP
                     VTFSTGSSGQTTLTIPASAKNIIAVGAYTTKESWVNIDGENLTLDTKGTIKIGDVTEF
                     SGAGPTRDGRTKPELTAPGRII"
BASE COUNT          310 a          592 c          567 g          348 t
ORIGIN      
        1 cgatgatccg gcctggcgcg gtcagctcgg gcttggtgcg gccgtcgcgc gtcggacccg
       61 caccggagaa ttcggtcaca tccccgattt tgatcgtccc cttggtatcg agggtgaggt
      121 tttctccatc gatattgacc cagctctcct tggtcgtata ggctccgacc gcgatgatat
      181 ttttggcgct ggcggggatg gtgagggtgg tctgaccgct gctccccgtc gaaaaggtga
      241 ccggcatggt tccgctggcg atccacatat cgaggcgctc actgctgccg ctgaagcgca
      301 gcttccactc ccccgcggcc ggcgccgtgc cggccccgtc gttgatctcg atgatgcact
      361 cccggtcgcc gttgaaggga ttggggccgc tgacgatctc ctcgccgctc tcgtagaaac
      421 cgttccagat gttgacctcg ccgctgcggg tattgcccga atagtagcgg ccatagccga
      481 ccgggccgat ggtctcatca gtgggggtga tgagagtgac agcggcctgg ctgccgccgt
      541 cataccagcc gtcaaggagg agatagtcgt ttccgctccc cgcagcgggc gtgtaggcgg
      601 ggatggtgaa ggagatcccg gcagtgccgg aggagggatt caccagggca tgatgcttct
      661 tgtcagcctc attgccggca gcggcgacga aaatgcgccc cggtccggag agggcatcga
      721 tatagcgctc caccgcggct gtgccatcgt gggcgccgaa ggtggtgccg aagctgagat
      781 tgatgatgca gggcatgccg gccgcagtcg ccacgctgtc gatgaatccg atcgcgatca
      841 tctggtcctc ggaactgaac tcggagacga agggatcacg gctggccttg acgatgacca
      901 ggccggcctc cggggccatg cccgcataac tccccagacc cgccccgctg ctgccgtccc
      961 cggccgcgat gccggcgaca tgggtgccgt gtccgctgta gtcatgggtg ggcagctccg
     1021 tgccggcggt cagggccgcc ttgatctgcg ccgcggtgac catggtgccg ccgtaatagg
     1081 cccccggctc gctcagatcc agaatcgccc tgatgcggct ctccccctcc ggggtgatga
     1141 aatcctggtg gcgccagtcg atgcccgaat cgatgatgcc gatgagcacc cccctgccgg
     1201 ccagcccgct ggcgttcctg gcggcgatgc cgccgacatc cggtatgctg acatcggcca
     1261 ccggacggga tttgacgccc gcttcgaggt aaagcagacc cggggtcccc gccacctcct
     1321 ccagaaggcc cggctcgacc gtcaggatgg ccaagtcacc gcgctgccag cgcactcgag
     1381 cgccccgttc aaccagggcc tccgccgaga actgcccgct ggcgatgacg gtgatacttt
     1441 cagcggaccc ggctttgccg aggccggctt cggaacccga ccgtcctttg aggagcttca
     1501 gctgaagaag cggatccagt cctgcgctcc tctctcctcc cggcgcagaa gagaccagcg
     1561 taaaaaccac caccccaatc atccacatca tttttttcaa tgttgtctcg ctgcaatggg
     1621 tacacttttc cacagctgaa atttatttca atccggactc aaatacaagc attaattatg
     1681 ccaggcggga gagggagcgc ggcactttcc tctttaaggg tcttcagcgc accaggagca
     1741 ttttcctgct ctgcacttcg cggccggcgc tgagacggca gagataaagg cctgccggca
     1801 ggccccgggc atcccat
//
LOCUS       DAOPWU010000533         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_71972_length_1802_cov_3.040069, whole genome shotgun sequence.
ACCESSION   DAOPWU010000533 DAOPWU010000000
VERSION     DAOPWU010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71972_length_1802_cov_3.040069"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..519)
                     /locus_tag="PKM23_15980"
     CDS             complement(73..519)
                     /locus_tag="PKM23_15980"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY93022.1"
                     /translation="MYSIIEEKCTGCGGCFDACQNQAIILVDGKAQIDYARCTECGSC
                     LDACRTGAIVFRSRALQKIATTGYDIDAGYDQRPAPLQSLTKGQLLLNLCDLFLKVAD
                     IFVDSYRKPVQKIDLAKPLPMQGRPLRQENGQRRHRGGGGRCGRSF"
     gene            complement(771..1790)
                     /gene="wecB"
                     /locus_tag="PKM23_15985"
     CDS             complement(771..1790)
                     /gene="wecB"
                     /locus_tag="PKM23_15985"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNY93023.1"
                     /translation="MESLICVTGQHRQMLDQVLELFGLHPDIDLNLMQPNQRLAILTA
                     RLFETLDSVLEETRPDWILAQGDTTTVFVSAVMAYYHDIRFGHVEAGLRTLDKRRPFP
                     EEMNRRLADAATDAWFAPTERARRALLTEGFPEAGIHVTGNTIVDALLEISQRSFDWR
                     KSALGGIPMEGRMVLITAHRRESFGEPFRELCQAIRELAGLFPEIHFVYPVHLNPNVR
                     RPVGEILDGLENVHLIEPVDYLTLVHLMKRSVLILTDSGGIQEEAPTFAVPVLVMRDT
                     TERPEGVEAGVARLVGTRREKIVAEAAAVLRNGSWIDNSQGTVNPYGDGQAARRIVDF
                     LRSTA"
BASE COUNT          416 a          510 c          480 g          396 t
ORIGIN      
        1 aggtgtcgtt aatggtcttc cccagcatat gcgtactggt gcagttcttc aaatccaggc
       61 cattcctcat ttctagaaac ttcttccgca cctgcccccg ccaccccggt gtcttctctg
      121 gccgttttcc tggcgcaatg gtcggccctg cataggcaga ggcttggcca agtctatttt
      181 ttgaactggt ttcctataac tatccacaaa gatatcggca accttcagga acaagtcaca
      241 aaggttgagc agcaactggc ccttggtcag ggactggaga ggggcaggcc gctgatcata
      301 acccgcatcg atatcatacc cagtcgtagc gattttttgc agcgcccggg atcgaaaaac
      361 aatggcgcca gtccggcagg cgtcgagaca gctgccgcac tcggtgcatc tggcatagtc
      421 gatctgggct ttcccgtcga ccaggataat cgcctggttc tggcaggcat caaaacagcc
      481 gccacaacct gtacactttt cttctataat gctgtacatg gtagaatcct cccctccgga
      541 atggaccgca aaaaatgtca aaaaattcag ttcattccag tatgacattt gcaaatatca
      601 taccaataca gcaatttaga atagttaggc ttaataatat atatttctta cgtttatatc
      661 aatacatcct gacatgaaag taaacatgat agttgcagaa ttgctacttt ttatttagta
      721 aggtagcaga aatgctctta tagcggggaa agcttccagc catcacgaaa tcaagcggtg
      781 ctcctcagga aatcgacaat gcgccgggcg gcctggccgt caccataagg attgacggtt
      841 ccctgcgaat tatcaatcca tgatccattc ctgagcacag cagcggcctc ggcgacgatc
      901 ttttcgcgcc gggtccccac cagccgcgcc actcccgcct ccaccccttc cggccgttct
      961 gtggtatcgc gcatcaccag cactggcacg gcgaaggtcg gcgcctcttc ctgaatcccc
     1021 cctgaatcgg tgagaatcag gaccgaacgt ttcatgagat ggaccagggt gagatagtcg
     1081 accggctcga tgagatggac attctccagc ccatcaagaa tttctccgac cggacggcgc
     1141 acattgggat tcagatgcac cggatagacg aaatgtattt cgggaaacaa gccggccaat
     1201 tcgcggatgg cctggcaaag ctcgcgaaac ggctcgccaa agctctcccg cctgtgggca
     1261 gtgatcagga ccatgcgccc ctccatcgga ataccaccga gggcactttt ccgccaatcg
     1321 aaagaacgct gcgagatctc gagcagggca tcgacaatag tgttgccggt gacgtggatt
     1381 cccgcttccg gaaatccctc tgtcagcagg gcccgccgcg cccgctcggt gggtgcaaac
     1441 caggcatcgg ttgcagcatc cgcgaggcgg cggttcatct cctccgggaa ggggcggcgc
     1501 ttgtcaaggg tgcggaggcc ggcttcgaca tggccgaagc ggatatcatg ataataggcc
     1561 atcacggcag agacaaagac cgtggtggta tcgccctggg cgaggatcca gtcgggtctg
     1621 gtctcttcga gaacactgtc cagggtctcg aacagccggg cggtgagaat cgccagcctc
     1681 tgattgggtt gcatcaggtt gagatcgatg tcgggatgga gaccgaaaag ctcgagcacc
     1741 tggtcgagca tctgcctgtg ctggccggtg acgcagatca ggctctccac ttcctgggga
     1801 tg
//
LOCUS       DAOPWU010000534         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_71975_length_1802_cov_2.949056, whole genome shotgun sequence.
ACCESSION   DAOPWU010000534 DAOPWU010000000
VERSION     DAOPWU010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71975_length_1802_cov_2.949056"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PKM23_15990"
     CDS             <1..682
                     /locus_tag="PKM23_15990"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNY93024.1"
                     /translation="KSDERIEKVMILGGGRVGRLVAAELEKDKDCQVKLVESNREKSL
                     RIAEELQRTLIIVGDGTDLDLLAVEGIMEMDSYIAVTSDEETNILSCLMAKHMGVRRC
                     LALVNRPDYLPIMASIGVDAAVDTQTLTANAILRYIRRGNVTSLASLPGIEAEVLEIE
                     ITRASRVAGRKVKALNLGHNGMIGIISRGGQVIVPIGDTELKDGDHLIVFTTPALVGR
                     VEKIFANR"
     gene            698..>1802
                     /locus_tag="PKM23_15995"
     CDS             698..>1802
                     /locus_tag="PKM23_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNY93025.1"
                     /translation="MNLRAVLHVVAILIILEGIALLTGIAFSLFYGDGDAGAILISSA
                     VCLLAGGLLFLATRNQDELKLREGFAVVVLGWSALTLFGALPFLISGTVPSFSDAFFE
                     TMSGFTTTGATILTNIEAMPHGLLFWRSLTHWLGGMGIVVLSLAILPLLGVGGMQLFK
                     AEVPGPVKDRLSPRIADTAKILWGVYLLLTLIEVVLLLFGGMSLFDSLCHTFGSMATG
                     GFSTKNASVAHYNSLYIEMVILVFMFLAGTNFSLHYRFLAGKRDVYRLDREFRFYLFL
                     TLIAILIVSGIVYFSKPVSPGGALRSASFQVVSIMTTTGFCSDNFELWLPSGQLLMIL
                     LMFIGGCAGSTGGSVKVIRIMVVLKHSLAEIKKL"
BASE COUNT          315 a          633 c          474 g          380 t
ORIGIN      
        1 caagagtgac gagcgcatcg agaaggtgat gattctcggg ggcggccggg tcggacgtct
       61 ggtggccgcc gaactggaga aggacaagga ttgtcaggtc aagctggtcg aatccaaccg
      121 cgagaagagc ctgcgcattg ccgaggaact gcagcgcacc ctcatcatcg tcggtgacgg
      181 caccgacctc gatctcctcg ccgtcgaggg gatcatggag atggacagct acatcgccgt
      241 caccagcgac gaggaaacca acatcctctc ctgcctcatg gccaagcata tgggagtgcg
      301 ccgctgcctc gccctggtca accggcccga ttatctgccc atcatggctt cgatcggagt
      361 cgacgctgcg gtcgacaccc agaccctgac cgccaacgcc atcctgcgct acatccggcg
      421 cggcaatgtc acctccctgg ccagcctgcc cggcatcgag gccgaggttc tcgagatcga
      481 gatcacccgg gcctcccggg tggccggacg caaagtcaag gccctcaacc tcggccataa
      541 cggcatgatc ggcatcatca gccgcggcgg ccaggtgatc gtccccatcg gcgacaccga
      601 gctaaaggat ggtgatcacc tcatcgtctt caccaccccc gccttagtcg ggcgcgtgga
      661 aaagatcttt gccaaccggt aagagcgaat ccgacctatg aacctccgcg ccgtcctcca
      721 tgtcgtcgcc attctcatca tcctggaagg gatcgcactg ctcaccggca tcgccttctc
      781 cctcttttac ggcgatggcg acgccggggc gatcctcatc tcctccgccg tctgtctgct
      841 tgccggcggc ctcctctttc tggctacccg caatcaggac gaactcaaac tgcgcgaggg
      901 tttcgccgtc gtcgtgctcg gctggagcgc cctcaccctc ttcggcgctc tgcccttcct
      961 catttccggg acggtaccct ccttctccga tgcctttttt gaaaccatgt cgggatttac
     1021 caccaccggc gccaccatcc tgaccaatat cgaggcgatg ccccacggtt tgctcttctg
     1081 gcgcagcctg acccactggc tcggcggcat gggcatcgtc gtcctctccc tggccatcct
     1141 gccactgctt ggcgtcggcg gcatgcagct cttcaaagcg gaagtgccag ggccggtcaa
     1201 ggaccgcctc agcccgcgca tcgccgacac cgcaaaaatt ctctgggggg tctatctgct
     1261 gcttactctc atcgaggttg tgctcctgct cttcgggggc atgtcgctct tcgattccct
     1321 ttgccacacc ttcggttcca tggccaccgg cggcttttcg accaaaaacg ccagcgtcgc
     1381 ccactataac agcctctata tcgaaatggt catcctggtc ttcatgttcc tcgccggcac
     1441 caacttcagc ctccactacc gcttcctcgc cggcaagcgc gatgtctacc gcctcgaccg
     1501 cgaattccgc ttttatcttt ttctcaccct gatcgcgatt ctcatcgtct ccggaattgt
     1561 ctacttcagc aagccggtca gcccgggcgg cgccctgcgt tccgcctcct tccaggtggt
     1621 ctcgatcatg accaccaccg gattctgctc cgacaatttc gagctgtggc tgccttccgg
     1681 acagctgctg atgatcctgc tcatgttcat cggcggctgc gccggctcga ccggcggctc
     1741 ggtcaaagtg atccgtatca tggtggtcct caaacacagc ctcgccgaga tcaaaaaact
     1801 gc
//
LOCUS       DAOPWU010000535         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_72087_length_1800_cov_5.755301, whole genome shotgun sequence.
ACCESSION   DAOPWU010000535 DAOPWU010000000
VERSION     DAOPWU010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72087_length_1800_cov_5.755301"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PKM23_16000"
     CDS             complement(<1..850)
                     /locus_tag="PKM23_16000"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNY93026.1"
                     /translation="MAKQPFLERVRERILVCDGAMGTMLYSRGIFINRCFDELNLSAP
                     EMVLDLHRAYAAAHADILETNTYGANRYKLKAHGLEEQTAAINRRGVELARQAADEEL
                     YVAGAIGPTGVTVAQLGTIPEAEMLELYGEQAAALAEAGVDLFLLETFQDLNEILLAL
                     RAVRRVSRLPVIAQMTYDEEGKTTLGVQPETVVQALLGEGADCIGANCSVGPHAMLEV
                     IRRLARAGAPFITAQPNAGLPRLFEGRLLYMSTPEYHAEYARRFISAGASVVGGCCGT
                     TPEHIRA"
     gene            complement(863..>1800)
                     /locus_tag="PKM23_16005"
     CDS             complement(863..>1800)
                     /locus_tag="PKM23_16005"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNY93027.1"
                     /translation="LDIGTGLYHNAYYYYSDYTGFVRDRNYGLSLLMSNPFNKFERIS
                     YGLSAMGISRSYLEIPDEIVDQLIDRGYLSARDRFFLMNNLTYTRDTTVWGYTGPTNG
                     GGMALGVTYSPGLGKSGIDFTTVRLDFRRYLRLMRDYTFATRLATGFSEGRNKQKFFL
                     GGLPNWLNQEFFGGIRVQHIEDVYFASFEMPLRGAEYYTLEGNRFALVNLEFRFPLIH
                     RAIIGFPLPIELGNIGGALFTDMGVAWDKGEKIKPFVKAPNGLFKTKDMFASFGFGVR
                     MNVGFFLLRYDLAWPTNFYNSSHSPQSLWSLGADF"
BASE COUNT          338 a          500 c          637 g          325 t
ORIGIN      
        1 tggcccggat atgctccggg gtggtcccgc agcagccgcc gaccacgctg gcgccggcgc
       61 tgatgaaacg gcgggcgtat tcggcgtgat actccggggt cgacatgtaa aggaggcggc
      121 cctcaaagag gcggggcagg ccggcgttgg gttgggctgt gatgaagggg gcaccggccc
      181 tggccaggcg ccggatcacc tcgagcatgg cgtgcggtcc gaccgaacag ttggcgccta
      241 tgcagtccgc cccctccccc agcagggctt gcaccacggt ctcgggctgg accccgaggg
      301 tagtcttgcc ctcctcgtca taggtcatct gggcaataac cggcagccgg ctgacccggc
      361 gaacggcgcg cagggcgagc aggatttcgt tgagatcctg gaaggtctcg aggagaaaga
      421 gatcgacacc ggcctcggcg agggcggcgg cctgttctcc gtagagctcg agcatctccg
      481 cctccgggat ggtgccgagc tgggccaccg tgactccagt gggtccgatc gcccccgcga
      541 cataaagctc ctcgtccgcg gcctggcggg ccagctcgac cccgcggcgg ttgatagccg
      601 ccgtctgctc ctccaggcca tgggccttga gcttgtagcg gttggcgccg taggtgttgg
      661 tctcgaggat gtcggcgtgg gcggccgcat aggcgcgatg gagatcgagg accatctcgg
      721 gcgcggagag gttgagctca tcgaagcagc ggtttataaa gatgccgcgg ctgtagagca
      781 tcgtgcccat ggcgccgtcg cagacgagaa tgcgctcccg gacgcgttcg agaaagggtt
      841 gcttcgccac agcgggcgtc tcctaaaagt ctgcgccgag cgaccacagg gattgcggcg
      901 aatgcgatga attgtaaaag ttggtcggcc aggccaggtc gtagcgcagc aggaaaaagc
      961 cgacgttcat gcgcaccccg aacccgaagc tggcgaacat gtccttggtc ttgaagagcc
     1021 cgttcggggc cttgacgaag ggtttgatct tttccccctt gtcccaggcc acgcccatat
     1081 cggtaaagag agcgccgccg atgttgccga gttcgatggg cagcgggaag ccgatgatgg
     1141 cgcggtggat caggggaaaa cggaactcga ggttgaccag ggcgaagcgg ttgccctcaa
     1201 gggtgtagta ctcagcgccg cgcaggggca tctcgaagga ggcgaaatag acatcctcga
     1261 tgtgctgcac gcggatgccc ccgaagaact cctgattgag ccagttgggc aggccgccga
     1321 ggaagaattt ctgtttgttg cggccctcgc tgaaacccgt ggccagacgc gtggcgaagg
     1381 tatagtcgcg catcaggcgc agatagcgcc ggaaatccag gcggacggtg gtgaaatcga
     1441 tgccgctttt accgagtcct gggctgtaag tgacaccgag tgccatgcca ccgccgttgg
     1501 tcggtccggt gtagccccag acggtggtgt cgcgggtgta ggtgagattg ttcatgagga
     1561 aaaaacggtc gcgtgcggag agatagccgc gatcgatgag ctggtcgacg atttcgtcgg
     1621 ggatctccag ataggagcgg ctgatgccca tggcgctgag gccgtagctg atgcgctcga
     1681 atttgttgaa ggggttgctc atgagcaggc tcaggccgta attgcggtcg cggacaaaac
     1741 cggtgtaatc ggaatagtag taataggcgt tgtgatagag cccggtgccg atgtcgagct
//
LOCUS       DAOPWU010000536         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_73492_length_1778_cov_4.320952, whole genome shotgun sequence.
ACCESSION   DAOPWU010000536 DAOPWU010000000
VERSION     DAOPWU010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73492_length_1778_cov_4.320952"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="PKM23_16010"
     CDS             complement(<1..1278)
                     /locus_tag="PKM23_16010"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNY93028.1"
                     /translation="MTRNMELRRREFLQVLGAGGAGLVLGVIRPVPLAGQEGAFVFKP
                     NEFIAIDRQGRVTIWVARSEMGQGARTAVPMMVAEELEADWEKITVVPALAHPTKYGS
                     MSTGGSTTVRRGWEPLRRAGAAAREMLITAAAQYWGVDRATCKAQAGTVLHMPSGRKL
                     DYGDLVVTASRLPVPQEVALKDPSTYRIVGSKRARLDTPEKLTGKAVFGFDFTFPGLL
                     VAVVARSPVFGGRFRSCDAAAALQVKGVQRVVRIPSGVAVVANSTWAALQGREKLVVE
                     WEEGEHAGLASPAIDDYFRRQSQLEGRVVRAEGDFAAAAAAAPTRLQAEYSVPFIAHA
                     PMEPMNCTADVRADRVEIHAPMQSPQGAQEAAAEITGLPLDQVHVHTTLIGGGFGRRL
                     MNDFVEEAVHLSKALGAPVKVVWSREDDMRHGFF"
     gene            complement(1275..1745)
                     /locus_tag="PKM23_16015"
     CDS             complement(1275..1745)
                     /locus_tag="PKM23_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNY93029.1"
                     /translation="MALYKLEVNGERVTVDVDPETPLLWVLRETLGLTGTKYSCGEGV
                     CGNCTVLMDGEAVRSCLLTAGDAVGKKILTIEGISRETDHPLLVAWREEEVSQCGYCQ
                     PGQIVAAYALLSQKPDPTDSEIDEAMSGLLCRCGTYPRIRRAIHRAAELKRRQP"
BASE COUNT          278 a          624 c          584 g          292 t
ORIGIN      
        1 gaaaaagccg tggcgcatgt cgtcctcacg gctccagacc accttgacgg gggcgccaag
       61 ggctttggag agatggaccg cctcctcgac gaaatcgttc atcagccggc ggccaaaacc
      121 cccgccgatg agggtggtat ggacgtggac ctgatcgagg ggcaggccgg tgatttcagc
      181 ggcagcctcc tgggccccct gcggtgactg catcggggcg tgaatctcaa cgcggtcggc
      241 gcggacatcg gcggtgcagt tcatcggctc catcggggca tgggcgatga aaggcacgct
      301 gtactcggcc tgcagccggg tcggtgctgc ggcggccgcg gcggcgaaat cgccttccgc
      361 gcgcaccacc cgcccctcca gctggctctg gcgccggaaa tagtcatcaa tggcagggct
      421 ggcgaggccg gcatgctccc cctcctccca ctccaccacc agcttctcgc ggccctgcag
      481 ggccgcccag gtggagttgg ccaccaccgc cacaccgctg gggatccgca ccacccgctg
      541 cactcccttg acctggaggg cagccgcggc gtcgcagctc cggaagcgtc cgccgaagac
      601 cgggctgcgc gcgaccaccg cgaccagcag gcccggaaag gtgaaatcga agccgaaaac
      661 ggccttgccg gtgagttttt cgggggtgtc gagccgcgcc cgtttgctgc cgacgatgcg
      721 gtaggtcgag ggatccttga gagccacctc ctggggcacg ggcagccggg aggcggtgac
      781 gaccagatcg ccgtaatcga gcttgcggcc gctcggcatg tgcagcactg tgcccgcctg
      841 ggccttgcag gtggcgcgat ccacccccca gtactgcgcc gccgcggtga tgagcatctc
      901 gcgcgcggcc gctcccgccc ggcgcagcgg ctcccagccc cggcggacgg tggtgctgcc
      961 gccggtggac atgctgccgt atttggtcgg gtgggcgaga gccggcacga ccgtgatctt
     1021 ttcccagtcc gcctccagct cctcggcgac catcatcggc acggccgtgc gcgccccctg
     1081 gcccatttcg gagcgggcga cccagatggt gacccgcccc tgccgatcga tggcgatgaa
     1141 ctcgttgggt ttgaagacaa aagctccctc ctggcccgcc agcggcacgg ggcggatgac
     1201 gcccagcacc aggccggcgc cgccggcccc gagcacctgg agaaattcac gccggcgcag
     1261 ttccatattt ctggtcatgg ctgcctcctt ttcagctccg ccgcccggtg aatggcccgg
     1321 cggatgcgcg gataggtgcc gcagcggcag agcaggccgc tcatcgcctc atcgatctcg
     1381 ctgtccgtgg ggtccggctt ttggctgagg agggcatagg cggcgacgat ctggccgggc
     1441 tggcagtagc cgcattggga gacctcctcc tcgcgccagg ccacgagcag gggatggtcc
     1501 gtctcccggc tgatgccttc gatggtgagg atcttcttgc ccacagcatc ccccgcagtc
     1561 aacaggcagg agcgcaccgc ctccccatcc atcagcaccg tgcagttgcc gcagaccccc
     1621 tcgccgcagc tgtacttggt gccggtcaaa ccgagggtct cgcgcagcac ccacagcagc
     1681 ggggtttcgg gatcgacatc tactgtcacc cgttcgccgt tcacctccag tttgtatagc
     1741 gccattttac ctccttgcgg taaaacatcg gtcagcaa
//
LOCUS       DAOPWU010000537         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_73695_length_1775_cov_3.601744, whole genome shotgun sequence.
ACCESSION   DAOPWU010000537 DAOPWU010000000
VERSION     DAOPWU010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73695_length_1775_cov_3.601744"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="PKM23_16020"
     CDS             <1..1594
                     /locus_tag="PKM23_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93030.1"
                     /translation="LCFLLGACGKKNSTAPEAPIDVGEFVQSTVQLTPQAWGIVDAAI
                     DEAVRRGTGINPDTIATNIRKLAMVASAEPTESGSAIIVKHKEGHYSTILLNLRDDDR
                     LFLPASAIRQTVEPSPPQSSLARPTLPAEHPTGNRVLILLPFQFSLKEDPARISAPFI
                     AAGFTVTVYANEEVTLERCRGDFLAGFHAIYFSTHGIGRGRTLGGRHSTILSTREPVT
                     EASLASLSDDEINALAVTIIHNKAYWGISIPWLEETLSIPFPGSWFYANACESAREDN
                     GSASFSAYLLTHGVGGFSGYDASINYLMTAEIDPLLVSWLLSGKSLADATHLVHTDLG
                     LLAHSWLLRNVYSAQKVDAELLDSSQRLTEPYYLLATGADFPYNTCIIDFFCRATYHH
                     STKGENSFAFGRLVEVKGKVLNGVFNGSALYKDEFKEESKTAEIQIDLVKHQITRFKI
                     TGYSKDIISTNWSISGTSVPLPGNTTNSYFYVKGTDFGNCAGKVEYFSSWKEPDGLTV
                     SEDLVRLNVDADSQISFQFSNR"
     gene            complement(1576..>1775)
                     /locus_tag="PKM23_16025"
     CDS             complement(1576..>1775)
                     /locus_tag="PKM23_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY93031.1"
                     /translation="PIILENDHGRAEFPHPKPDHVQQFLIENIVAALRGEAEPLSSGE
                     SGAHTSAVMEEMVRGYYRFEN"
BASE COUNT          385 a          542 c          473 g          375 t
ORIGIN      
        1 gctttgcttt ctcctgggag cctgcgggaa gaaaaacagc accgcgccgg aggcgcccat
       61 cgatgtcggg gaattcgtgc aaagcacggt gcagctcacc ccccaggcct gggggatcgt
      121 cgatgcggct atcgatgagg ctgtacgccg cggaacaggc attaacccgg acaccatcgc
      181 cacaaacatt cggaagctgg cgatggtcgc atcggccgaa ccaacggaga gcggctcggc
      241 cataatcgtc aagcacaaag aaggccatta cagtacgatc cttctcaacc tgcgtgatga
      301 tgaccggctt ttccttccag cctcagcgat acggcagaca gtggaaccct cgccgccgca
      361 atcttctctg gccaggccca cacttcccgc ggaacatccg accggcaacc gggtcttgat
      421 tctcctgcct ttccagttct ctttaaagga ggatccggcc cgcatctccg ctccattcat
      481 cgccgccggt tttacagtca cagtctacgc taacgaggag gttaccctcg agcgctgccg
      541 aggtgatttt cttgccggtt tccatgccat ctatttctca actcacggca ttggccgagg
      601 ccgcactctc ggcggccggc attccacaat cctcagcaca cgtgaacctg tcactgaagc
      661 ctccctggcc tctctcagcg atgacgagat caatgccctg gctgtcacaa tcattcataa
      721 caaagcctac tggggaattt cgatcccctg gctcgaagag actctttcca tcccctttcc
      781 agggagctgg ttctatgcca atgcctgcga aagtgcaaga gaggacaacg gcagcgcctc
      841 cttcagtgct tacctcctca cccacggcgt cggcggcttt agcggatatg atgcctccat
      901 aaattatctg atgactgcag agatcgatcc cctgctggtg agttggctcc tctccggcaa
      961 gtcgctggcc gatgcgacgc acctggtgca cacagacctc ggcctcctgg cccacagctg
     1021 gctgctgcgc aatgtctaca gcgcccagaa ggtggatgcg gagctgctcg actccagcca
     1081 gcggctgaca gagccctact acctgctcgc aaccggtgcg gatttcccct ataatacttg
     1141 catcatcgat tttttctgca gggcgaccta tcatcattcg accaagggag aaaactcatt
     1201 cgccttcggc cgtctggtgg aggtgaaggg caaggtcttg aacggggttt tcaatggcag
     1261 cgccctttac aaggatgaat tcaaggagga gagcaagacc gctgaaatcc agatcgatct
     1321 ggtcaagcat caaatcaccc ggttcaagat caccggatac agcaaggata tcatcagtac
     1381 caactggtct atttccggca ccagtgtgcc cctgccaggg aatacgacca acagctattt
     1441 ttacgtcaag gggacggatt tcgggaactg cgcgggcaag gtagagtatt tcagcagctg
     1501 gaaagaaccg gatggcctga ccgtcagtga agatttggtg cgcctgaatg tcgacgccga
     1561 cagccaaatc tcgtttcaat tctcaaaccg gtaatacccc ctgaccatct cttccatcac
     1621 cgcgctggtg tgcgcgccgc tctcgccgct ggagaggggt tccgcctcgc cacgcagggc
     1681 ggcgacgatg ttctcgatga ggaactgctg gacgtgatcc ggcttgggat gagggaattc
     1741 ggcacggcca tggtcattct ccagaatgat cggca
//
LOCUS       DAOPWU010000538         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_73776_length_1774_cov_2.795230, whole genome shotgun sequence.
ACCESSION   DAOPWU010000538 DAOPWU010000000
VERSION     DAOPWU010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73776_length_1774_cov_2.795230"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PKM23_16030"
     CDS             complement(<1..974)
                     /locus_tag="PKM23_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93032.1"
                     /translation="MKKNHCFFLFTGLLLFITTPLFSIDIINLHRNDAAGRPASPYTI
                     NTVVTVSGVVTVPSGLFSPTTFDVVIQDSTAGIHLFSTRGDLAFVQLGDLIRVTGPIS
                     HLYGLTYIKEPTEITLLARSQSLPEPLLLTCAEVAGTFKADNSEPNESRLIRVDHVAV
                     TGSGGDLYTISDATGTAQLYLDPDAGLPSLPDGHFDLIGILKQVDTSTRPPVGRGGYR
                     IMPRYSSDIISRGAPLLVRPLQEAEINPRAVLLSWHTDRPATSLVRFGSASFRERAAG
                     DSTLVTDHKVLLDGLEPASLYRACAWSSDSAGTMISDSLLFMTASGGS"
     gene            1196..1705
                     /locus_tag="PKM23_16035"
     CDS             1196..1705
                     /locus_tag="PKM23_16035"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNY93033.1"
                     /translation="MDISHDLLTQEYIEVIRDLEKKNRVARIKDIASVRGVTCANVSV
                     AMTNLARKELVVHEQYGHVVLTSAGRRLARNLEKRHLAIRRFMTDVLGLETELAESEA
                     CKLEHVMSPLAMQAISRFLLFVERCPKRDRENVVLFRLCGLFGDSAADCSECAQEGNE
                     EENPAEDEF"
BASE COUNT          399 a          450 c          572 g          353 t
ORIGIN      
        1 cttgagcctc cggaggcggt catgaagagc aaggaatcgg agatcatcgt gcccgccgag
       61 tcgctcgacc aggcgcaggc gcggtagagg ctcgcgggtt caagtccatc gaggagtacc
      121 ttgtggtcgg tgacgagggt tgaatcacct gcagcccgtt cacgaaagga cgcactgccg
      181 aagcgcacca gggaggtggc gggacggtcg gtatgccatg acagcaggac ggcgcgggga
      241 ttgatttcag cctcctgcag ggggcgtacc agcagggggg cgccgcgcga gatgatatcg
      301 ctgctgtagc ggggcatgat acgatacccc cccctgccga ccggcggccg ggtgctggtg
      361 tcgacctgct tgaggatgcc gatgaggtcg aaatggccgt ctggaagcga gggcaggccg
      421 gcatccggat cgaggtagag ctgggcggtg ccggtggcgt cgctgatggt gtagagatca
      481 cccccggaac cggtgaccgc gacatgatcg acgcggatga ggcggctttc attgggttcc
      541 gagttgtccg ctttaaaggt gccggcgacc tcggcgcagg tcaggagcag gggctccggc
      601 aggctctggc tgcgggccag cagggtgatc tcggtgggtt ccttgatata ggtcagtccg
      661 tagagatgac tgatgggacc agtcaccctg atcagatcgc ccagctggac aaaggccaga
      721 tcccctctgg tagaaaaaag gtgtatgccg gcggtgctgt cctgaatgac gacatcgaag
      781 gtggtgggtg aaaagagccc ggagggcacg gtcaccacac cggagacggt caccacggtg
      841 ttgatcgtat agggcgaggc cggtctgccg gcggcatcat tacggtgcag gttgatgata
      901 tcgatggaaa aaagcggggt agtaatgaaa agcaggagac cggtgaagag gaaaaaacag
      961 tgattctttt tcatcaaggt tccaatctgc aggcagcata ctccgaataa gacctgatcc
     1021 tgcgtgacca tcgcagacag tctggtgttg cctataaata tagctattac cctactcttt
     1081 gtcaacacta aaaccgcctt aaaaaaaggt tgcagatgtg ctggaaggtt cgtaaattta
     1141 ggccgtttaa ttatgttaat ttgatttaac tttacggctt gtgccgggat aaagcatgga
     1201 catctcacat gatctcctga cccaggaata catcgaggtc atccgcgatc tggagaagaa
     1261 gaaccgcgtt gccaggatca aggatatcgc ctcggtgcgc ggcgtcacct gcgccaatgt
     1321 ctcggtagcc atgactaatc tggcccgaaa agagctggtt gtgcatgagc aatatggtca
     1381 tgtcgtcctt accagcgccg gccggcgtct ggcgcgcaat ctcgagaaac ggcacctggc
     1441 gatcaggcgg ttcatgacag atgtgctcgg tctggaaacc gagctggcgg agagcgaagc
     1501 ctgcaaactg gagcatgtaa tgagtcccct ggccatgcag gcgatcagca gatttctgct
     1561 tttcgtcgaa cgctgcccca aacgcgaccg ggaaaatgtc gtcctgtttc gtttgtgtgg
     1621 tcttttcggc gacagcgctg ccgattgcag cgagtgtgcc caagagggca atgaagagga
     1681 gaatccggct gaagatgaat tttagatttc ggcactgcga gcacaaacgg acgaaatcca
     1741 gcgccctccg cctctccgag atgcacaccg gcca
//
LOCUS       DAOPWU010000539         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_74172_length_1768_cov_4.977233, whole genome shotgun sequence.
ACCESSION   DAOPWU010000539 DAOPWU010000000
VERSION     DAOPWU010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74172_length_1768_cov_4.977233"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..937)
                     /gene="rlmB"
                     /locus_tag="PKM23_16040"
     CDS             complement(203..937)
                     /gene="rlmB"
                     /locus_tag="PKM23_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNY93034.1"
                     /translation="MYSYIYGRNPVLEWLRAGYPAEKLLVSRELGGVALQEISKLAAA
                     GPIPVESLPRNELARVAGSDNHQGVAARVTLPDYVEVDDILATASGRNEPPLIALLDC
                     IQDPHNFGAILRTAEGAGVHGVLIPKDNAAPMTPAVFKASAGAAAHVAVARITNLSRT
                     MAELKEKGFWFTGADEEGSKLYTELDLRGPTGLVLGSEGKGLRRLVREECDFVARIPL
                     RGKVSSLNVSVAAALLFFEARRQRGF"
     gene            complement(1009..>1768)
                     /locus_tag="PKM23_16045"
     CDS             complement(1009..>1768)
                     /locus_tag="PKM23_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNY93035.1"
                     /translation="IRNFTTVSENLTPEILVAQLNDYLTGMTEVVFKYQGYLDKYEGD
                     AIMAVYGVPVEMQDHARRACLAALEMQRELVGLRERWRREHKPEFHVRMGINSGPMIA
                     GNIGGKERFDYTVIGDSVNLASRLEGANKNYGTSIMISEFTRELLGDEFPLRELDLLR
                     VKGKNQPVRVYELLGSSAAAVSAEKRQAIAAYEEGLTLYRQKKWDAAEAAFQRALAAD
                     PEDGPSQTYIERCRYFRVNPVEENWDGVFEMKTK"
BASE COUNT          307 a          577 c          539 g          345 t
ORIGIN      
        1 ccagtcactg tgttccccga agaggcagat ccgccccggc acaaatagct tcattgactt
       61 ttctcccata aggaataaac cgttaagccc caaaaagagg ctctctcacc aagccaccaa
      121 ggcaccagga gaaaccaggc cgatgcagcc ttgtttttgc tttgtggctc tgtggctttg
      181 tgagagatgg tattgcatag aatcagaatc cccgctggcg gcgggcctcg aaaaagagca
      241 gtgcagccgc caccgagaca ttgagggaac tcaccttgcc ccgcagcggg atgcgggcga
      301 cgaaatcgca ctcttcgcgc accagccggc gcagcccctt gccctcggat cccagcacca
      361 gtcccgtggg tccgcgcaaa tcgagttccg tgtagagctt gctgccctcc tcgtcggcgc
      421 cggtgaacca gaaccccttc tccttgagct cagccatggt ccggctcaga ttggtgatac
      481 gggcgacggc gacatgggcg gcggcccctg ccgaggcttt aaagaccgca ggggtcatcg
      541 gggcggcgtt gtccttgggg atgaggaccc cgtgcacccc ggccccctcg gcggtgcgca
      601 ggatggctcc gaagttgtgc gggtcctgga tgcagtcgag gagggcgatc aggggcggct
      661 cgttccgccc cgacgctgtc gccaggatgt cgtcgacctc gacgtaatcg ggcagggtga
      721 ccctcgcggc cacaccctga tggttgtcgc tgccggcgac cctggccagc tcgttgcgcg
      781 gcagagactc gaccgggatg ggcccggcgg cggcgagctt gctgatctcc tgcagggcga
      841 caccgcccag ctctcgcgag accagcagct tttcagcggg ataaccggcc cgtagccact
      901 ccagcaccgg gttgcggccg tagatatagg aatacatcgc tattttctcc ttgaatggcc
      961 attcgccccg gccttgggcc tgtgccgtgc aaaggcggat gtgcgcctct acttggtctt
     1021 catctcaaag accccgtccc agttttcctc cacgggattg actctgaaat agcggcagcg
     1081 ctcgatgtag gtctggctcg gcccgtcctc cgggtcggcc gcaagggcgc gctgaaaggc
     1141 cgcttcagcc gcgtcccact tcttttgccg gtaaagggtg agcccctcct cgtaggctgc
     1201 gatcgcctgc cgcttctccg cactcaccgc ggccgctgag ctgccgagca gctcatatac
     1261 ccgcaccggc tggttcttgc ccttgacccg cagcaggtcg agttcgcgca gcggaaattc
     1321 atccccgagc agctcgcggg tgaactcgct gatcatgatg ctggtgccgt agttcttgtt
     1381 ggccccctcg aggcgcgagg cgagattgac cgaatccccg atcacggtat agtcaaagcg
     1441 ctccttgccg ccgatattgc cggcgatcat cggaccggag ttgatcccca tgcgcacgtg
     1501 gaattcgggc ttgtgctcgc ggcgccagcg ctcgcgcagc ccgaccagct cgcgctgcat
     1561 ctccagggcg gccaggcagg cgcggcgggc gtggtcctgc atctccaccg gcacgccgta
     1621 gaccgccatg atcgcatccc cctcatattt gtcgaggtag ccctgatatt taaagaccac
     1681 ctcggtcatg cccgtgagat agtcattgag ctgggcgacg aggatctccg gggtgagatt
     1741 ttccgaaacc gtggtgaaat tcctgatg
//
LOCUS       DAOPWU010000540         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_74238_length_1767_cov_4.898364, whole genome shotgun sequence.
ACCESSION   DAOPWU010000540 DAOPWU010000000
VERSION     DAOPWU010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74238_length_1767_cov_4.898364"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..>1767
                     /locus_tag="PKM23_16050"
     CDS             479..>1767
                     /locus_tag="PKM23_16050"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNY93036.1"
                     /translation="MYTLWRYIIKEHIGPFLFAICVISLVFILNLVFRELPRILSRGL
                     DLPLILEFFALNMAWIVALAVPMAVLVAALMAFGRLAGDNEIVAMKASGISVLRMITP
                     VLICAAGLALFLIWFNNQVLPDFNHRARLLASDIARKRPTLSLEAGVIYRDLPNYTLL
                     VQHIVETPDTAYVDSVYIDDNSNPNSSTFITADRGKIYLNPRNGQLSIILYDGVMYEL
                     NLEEMEAYRQLRFPKQVLSISVPDMVMERSESEYRGDREKSSAMMRDEVTVNNREIAT
                     ASARMAKKMHAHFARYLPPPDSLLTAGAHAENPLALPLQASPLLAENQRLRQEITADL
                     SFVRGLDRKNYQLLVEIHKKYSIPVACLIFVLVGAPLGMMARRGSMAMAAGISFSFFL
                     LYWASLIGGEELADEQLISPFMAMWLANIIVGIAGIYL"
BASE COUNT          358 a          593 c          435 g          381 t
ORIGIN      
        1 cgcaggcgga cgcatgccga tatagggcgc aggctgggca tcatctgccc acacctgtcc
       61 caccaggctg acgaaaatga ccaccgaaag caccaccgca ggcagcaggg tgaccatcga
      121 tgaatggatt cgacgtttgg ccatgcgctc gtagagtaag agggaaaacc ggccggattc
      181 ggcccttttt aggtgttacg tcggtagcga aagggcgata caatctctcc ctaatagttt
      241 gtttctaaaa cagttcccgc tggtgcggga tttgcctgcg gtggattata agtgaaaatg
      301 tacatatttt ctcttacaat atcaataaaa aataccctgc ccccggcaaa aagttcctga
      361 tttttatagc atgtcttgtt tttttttaat actttatctc atctatttcc cgcaccttgc
      421 cgggataacg gtgcaatatg cataccaact tttatgccat ctccatctag gttgagcgat
      481 gtacacactc tggcgctaca tcatcaagga acacatcggc ccctttctct ttgccatctg
      541 cgtgatcagc ctggtcttca tcctcaacct ggtctttcgc gagctgccgc gcatcctcag
      601 ccgcggcctc gatcttcccc tgatcctcga attcttcgca ctcaacatgg cctggatcgt
      661 cgctctcgcc gtccccatgg ccgtcctcgt cgccgccctc atggccttcg gccgcctcgc
      721 cggcgacaac gaaatcgtcg ccatgaaagc cagcggcatc agcgtcctgc gcatgatcac
      781 cccggtgctg atctgcgcgg ccggactggc cctctttctg atctggttca acaaccaggt
      841 cctgcccgac tttaatcatc gcgcccgcct gctcgcctcc gatatcgcgc gcaaacggcc
      901 gaccctctcc ctcgaggccg gggtgatcta ccgcgacctg cccaactata ccctgcttgt
      961 gcagcacatt gtcgaaaccc ccgataccgc ctatgtcgac tcggtctata tagacgacaa
     1021 ctccaatccc aactcgagca ccttcatcac cgccgaccgc ggcaagatct acctcaaccc
     1081 gcgcaacggc cagctcagca tcatcctcta cgacggtgtg atgtacgaac tcaacctcga
     1141 agagatggag gcctaccgcc agctgcgctt tcccaagcag gtgctctcca tcagcgtgcc
     1201 ggacatggtc atggagcgca gcgaatcgga ataccgtggc gaccgcgaga agagctcggc
     1261 gatgatgcgc gatgaggtga cggtcaacaa ccgggagatc gccaccgcct ccgcacgcat
     1321 ggccaaaaag atgcacgccc actttgcccg ctatctgccg cccccggaca gcctcctcac
     1381 cgccggggcg catgcggaaa atcccctcgc actgcccctg caggcctcac ccctgctggc
     1441 cgaaaaccag cgcctcagac aggaaatcac cgccgacctc agttttgtcc ggggcctcga
     1501 ccgcaagaat taccagctgc tggtggagat ccacaagaaa tactccatcc ccgtcgcctg
     1561 tctgatcttc gtgctggtcg gcgccccgct cgggatgatg gcccgccggg gctcgatggc
     1621 catggccgcc ggcatcagct ttagcttttt cctgctctat tgggcctccc tcatcggcgg
     1681 cgaggagctg gccgacgagc agcttatctc ccccttcatg gcgatgtggc tggccaacat
     1741 catcgtgggc atcgccggca tctatct
//
LOCUS       DAOPWU010000541         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_74241_length_1767_cov_4.730140, whole genome shotgun sequence.
ACCESSION   DAOPWU010000541 DAOPWU010000000
VERSION     DAOPWU010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74241_length_1767_cov_4.730140"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="PKM23_16055"
     CDS             complement(<1..1551)
                     /locus_tag="PKM23_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93037.1"
                     /translation="MKKIILLLLALAGTLPAFENPRLAIVISRTSFDQKWGVTQMAAH
                     GWGAAANLAGIPYDCFFFEDLAAADLSRHQTMILAQCSYVTSAGYDGSLAALRRYLAR
                     GGHLIIDGPLAVYDDKAQPIDHAELDSLVGVRYDGFRGSEMSRIRVGDADHFITRDFA
                     PEQYLTQHLTGGLNILGGGRALLVSGDEKASYPFLSVQNREKSRIVLVSDFSTWAGAA
                     SFFRNVQPQVFYRNELFNVMIRAIHWTLYGEPASPFPVPQISNANLTAIVRLDADGSG
                     NLSEQITTINYLTEIARESGVVPVYAWVSSQATKAGWQDLAPYGKKIEEVGGEIGTHS
                     RFHRIDEAMTEERWRDELDGSIHEIEFNTADYGYSIGKVECMINPGNTIRMSDYEQVA
                     SRFSFYMTHGFEQDMPLGFGNFTWYTGKHKNLALLEDTPSPDYQWFYDPTWSYTTQQI
                     TAYQEAIFDHLFERIGRGVVYNQMWHDYSITSQPQYGKSRIVNSSNIAMYDAIRRKFA
                     SKEIYAPAP"
     gene            complement(1564..>1767)
                     /locus_tag="PKM23_16060"
     CDS             complement(1564..>1767)
                     /locus_tag="PKM23_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93038.1"
                     /translation="FNGLEGGRNANQTPVRAARVVLPAAAGSRVHFLPFLGQVQILPE
                     EPVNGMISCTLPEFQRGAILWVQ"
BASE COUNT          353 a          490 c          568 g          356 t
ORIGIN      
        1 cggagcaggc gcatagatct ccttgctggc gaatttacgg cgaatggcgt catacatggc
       61 gatgttgctg ctgttgacga tgcggctttt gccgtattgg ggctgcgagg tgatcgaata
      121 gtcatgccac atctggttat agacgacgcc gcggccgatg cgttcgaaga ggtgatcgaa
      181 gatggcctcc tggtaggccg tgatctgctg ggtggtgtag ctccaggtcg gatcgtaaaa
      241 ccactgataa tccggggaag gggtgtcctc cagaagcgcc aggtttttat gcttgccggt
      301 ataccaggtg aaattgccaa aacccagggg catgtcctgc tcaaagccgt gggtcatata
      361 gaaggaaaac cggctggcca cctgctcata atcactcatc ctgatggtat tgccgggatt
      421 gatcatgcac tcgaccttgc cgatagagta gccatagtcg gctgtgttga actcgatctc
      481 atggatcgaa ccgtcgagtt catcgcgcca gcgctcctcg gtcatggcct cgtcgatgcg
      541 gtggaatcgg ctgtgagtgc caatctcgcc cccgacctcc tcgatctttt tgccgtaggg
      601 ggccagatcc tgccagccgg ccttggtcgc ctggcttgag acccaggcgt agaccggaac
      661 gaccccgctt tcgcgggcga tctcggtcag atagttgatc gtggtgatct gctcgctgag
      721 attgccgctg ccatcggcat cgagacggac gatggcggta aggttggcat tggagatctg
      781 cgggaccgga aagggagagg cgggttctcc atagagagtc cagtgaatgg cgcggatcat
      841 gacattgaaa agctcattgc gatagaatac ttgcggctgg acgttgcgga agaatgaggc
      901 ggctcccgcc caggtggaaa aatcgctgac gaggacgatc cggctcttct cccggttctg
      961 gactgaaaga aagggatagc ttgctttctc gtcgccgctg acgagcaggg cgcggccgcc
     1021 gccgaggata ttgagaccgc cggtgaggtg ctgggtgaga tactgctccg gtgcgaaatc
     1081 acgggtgata aagtgatccg catccccgac gcggatgcgg ctcatctcgc tgccgcggaa
     1141 gccgtcgtag cgcaccccga ccagggagtc cagctccgca tggtcgatgg gctgggcctt
     1201 gtcatcatag accgcgaggg gtccatcgat gatcaggtgg cctccccgcg ccagatatcg
     1261 cctgagcgcg gccaggctcc cgtcataccc ggcggaagtg acatagctgc actgggccag
     1321 aatcatggtc tgatggcggc tgaggtccgc cgcggccaga tcttcgaaga aaaaacagtc
     1381 ataggggatg cccgccagat tggccgcggc gccccagccg tgcgcagcca tctgggtcac
     1441 cccccatttc tggtcgaagc tggtgcggct gatgacgatt gccaaccgcg gattttcaaa
     1501 ggcgggcaga gtgccggcga gagcgagcag caacaggatg atctttttca taggaccttt
     1561 ctctcattga acccaaagaa tcgcgccgcg ttgaaactcg ggcagtgtgc agctgatcat
     1621 gccgttaacc ggctcctccg ggaggatctg tacctgcccc agaaagggca gaaagtggac
     1681 ccggctgccg gccgccgccg gcagaaccac acgggcagcg cggacagggg tctggttggc
     1741 attgcggccg ccctccagac cgttgaa
//
LOCUS       DAOPWU010000542         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_74655_length_1761_cov_3.949590, whole genome shotgun sequence.
ACCESSION   DAOPWU010000542 DAOPWU010000000
VERSION     DAOPWU010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74655_length_1761_cov_3.949590"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PKM23_16065"
     CDS             <1..1088
                     /locus_tag="PKM23_16065"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNY93039.1"
                     /translation="MNKRVFLTLLGLAAISAGLQAQTWDLKLKDWQPVSQLVTKQTLI
                     EKAKFPVIDIHNHLGKLENTAAYLAEMDKAGVWKCVSLDARSKDDFYKKHLKASREVS
                     PDRFLLFFKPDFSRIDEVNFGQNEAKRLEEAVKMGCRGLKISKSLGLSDKDSTGKLIT
                     VDDPRIDPIWAKCGELGIPVEIHVTDPKAFFTPVDRFNERYDELGMNPEWSFYGDQYP
                     EKEEILAARNRVIARHPNTIFIGAHFGNLPEDLATVAGWLESWPNFYVDIDARISELG
                     RQPYSARRFFLKYQDRILFGTDTPCRADAYRLYFRFLETDDEYIDPTPAHKLQGRWMI
                     YGLYLPDEVLEKIYNKNALKILALFKG"
     gene            1492..>1761
                     /locus_tag="PKM23_16070"
     CDS             1492..>1761
                     /locus_tag="PKM23_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93040.1"
                     /translation="MFKRLVVQSIMASGLLLTAFYAAGMAQEVDTLRLGRKVVPLMQE
                     IHLNLDPAQEIYNGTVDIALDVCEQTDYFRLHTKMLEIGKLVLQRA"
BASE COUNT          370 a          514 c          468 g          409 t
ORIGIN      
        1 agatgaacaa gcgggtgttt ctcactctcc tgggcctggc cgccatcagc gccgggctgc
       61 aggcgcagac gtgggatctc aagctgaaag actggcagcc ggtcagtcag ctggtcacca
      121 agcaaaccct gatcgaaaag gccaaatttc cggtgatcga catccataac catctgggca
      181 aactggagaa caccgcggcc tatctggcgg agatggacaa ggccggggtc tggaagtgcg
      241 tcagcctcga cgcccgctcc aaggatgatt tttacaagaa acacctgaag gcatcccggg
      301 aggtctcccc ggatcgtttt ctgctctttt tcaaacccga tttcagccgt atcgatgagg
      361 tcaatttcgg ccagaacgag gccaagcggc ttgaggaggc ggtgaagatg ggctgtcgcg
      421 gattaaagat ctccaaatcc ctcggcctgt ctgacaagga cagcaccggc aaactgatca
      481 cggtcgacga tccgcgcatc gacccgatct gggccaagtg cggcgagctg ggcatcccgg
      541 tcgagatcca cgtcaccgat cccaaggcct tcttcacccc ggtcgaccgc ttcaacgagc
      601 gctatgacga actcggcatg aatccggagt ggtcctttta cggcgatcaa taccctgaaa
      661 aagaggagat cctggcagcc cgcaaccgcg tcatcgcccg ccatcccaat acgatcttca
      721 tcggcgccca tttcggcaat ctgcccgagg atctggccac cgtcgccggc tggctggaga
      781 gctggccgaa cttttatgtc gacatcgacg cccgtatcag cgaactcggc cgccagccct
      841 acagcgcacg gcgctttttc ctcaaatacc aggaccggat cctcttcggc accgacaccc
      901 cctgccgggc cgacgcctac cgcctctatt tccggttcct cgagaccgac gacgagtaca
      961 tcgatcccac gccagcccac aagctgcagg gccgctggat gatttacggg ctctacctcc
     1021 cggatgaggt gctggaaaag atctacaaca aaaatgccct gaagatcctg gccttgttca
     1081 agggctgaga aatgcacgga gctgtgcggg cggcggtcct cttgcggccg ctgctcgcct
     1141 gtttggcgtt tatccctgac gaggccgggc cagtccggcg ccgcttttgc tatcgctgcc
     1201 cggtttttac cggctgggct gtcgaataat tgtgtatttt gattttcact tgacaggaac
     1261 agacgcgtgt attaaattat attatcgacc tcggggtgct cttcaaaatc gtctcattcc
     1321 tatcctgctt attatattct cattccttct ggatccccta ttctttcccg agctggaccg
     1381 gactggtttt ctaccattct ttttctgcat tctcataatc attttaatgc tccggcagct
     1441 cgccgcaggc ctgtaattgc ggcctgctgt aaaattctgg agggctgatt gatgttcaag
     1501 cgattggtag tgcagtctat catggccagt ggcctcctgc tgactgcttt ttacgcggct
     1561 ggcatggctc aggaagtgga taccttgcgc cttggccgaa aggtggttcc gctgatgcag
     1621 gaaatccatt tgaatctgga tcctgctcaa gagatctata acgggactgt cgatattgcc
     1681 ctggatgtct gtgagcaaac agattatttc cggctgcaca ccaagatgct ggaaattggc
     1741 aaacttgttc ttcaaagagc g
//
LOCUS       DAOPWU010000543         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_76157_length_1737_cov_5.623068, whole genome shotgun sequence.
ACCESSION   DAOPWU010000543 DAOPWU010000000
VERSION     DAOPWU010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76157_length_1737_cov_5.623068"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PKM23_16075"
     CDS             complement(<1..966)
                     /locus_tag="PKM23_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93041.1"
                     /translation="MKRGILFLILYFSMIFTLTAQTASPFVPLMDWGHWRLGHMSDLE
                     FLHKNNMTITFGSGAPNFETVSRADFDQQMAEARAFNQKYHDLGYTVLRYLSTSLNGT
                     SATAEDKPQKEQIHMLRFYNENWEEYADYLGPKPQAQDNPTTWITIHPDGDFPFYRYA
                     PYGEKVRPGFETWGCPDNPYYVHMMEGRVRAQAECGIDGSYVDWTQIAGNTCYCEYTR
                     KAFIGYLEEHLPAGVARAKYGVDSFEEIALPQKRGDRFWMEWLIFRGRSVAHFHGRLL
                     AAAHKVNPHFQIAGNVYGGFGYGPIAYDAAGNFEELGETDTFLYSE"
     gene            complement(995..>1737)
                     /locus_tag="PKM23_16080"
     CDS             complement(995..>1737)
                     /locus_tag="PKM23_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93042.1"
                     /translation="LRLMAQWDYSWRAEKDKLFLTLDLTRVNLDTMPGFSGGMGIAID
                     NCDQFIQEVLLDGRIHDAFSDRLIILPNLSRGKHSFEIRLGDRPALQPHLAYISQIMP
                     SCRRSGDELIVEVRTRNRARFRFEAATDLLLLNADAQSRDRLQGTRLSGHATSDRQIV
                     LAHLPAPLQEVTRATMAIAAARKMKKSVLFDLKANALEENQLWLRAGDRPKQVLLSGK
                     PVPWSVVEGEIRIALGPAKGDTQLSIEF"
BASE COUNT          337 a          494 c          541 g          365 t
ORIGIN      
        1 ctccgaatag aggaaggtgt cggtctcgcc gagttcttca aaattgccag cggcgtcgta
       61 ggcgatcggg ccgtatccaa aaccgccata gacgttgcct gcgatctgaa aatgaggatt
      121 gaccttgtgc gccgcggcga gaagacgtcc atgaaaatgg gcgactgaac ggccccggaa
      181 gatcagccac tccatccaga acctgtcgcc ccgcttctgc gggagggcga tctcctcaaa
      241 actgtcgacg ccgtattttg ccctggccac tccggcgggc agatgttcct caagatagcc
      301 gataaaggct ttccgggtat actcacagta gcaggtattg cccgcgatct gggtccagtc
      361 gacgtacgaa ccatcgatgc cgcactccgc ctgggcgcgc acccggccct ccatcatgtg
      421 cacatagtat ggattatccg gacaccccca ggtttcgaaa ccgggtcgca ccttttcgcc
      481 gtagggcgca tagcgataaa aggggaaatc cccgtccgga tggatggtaa tccaggtggt
      541 cggattgtcc tgcgcctggg gtttggggcc gagatagtcg gcgtactcct cccagttctc
      601 attgtaaaaa cggagcatat ggatttgctc tttctgcggc ttgtcttcgg cggttgcgct
      661 ggtgccgttc agagaggtgg agagatagcg cagcactgtg tagcccagat cgtgatattt
      721 ctggttgaag gccctggcct cggccatctg ctgatcgaaa tcggcgcggc tgacggtctc
      781 aaaattgggg gcgccgctgc caaaggtgat ggtcatgttg tttttatgca agaattccag
      841 gtccgacatg tgccccagcc gccaatggcc ccagtccatc aggggtacaa acggtgaggc
      901 ggtctgggct gtcagggtga agatcatgct aaaatacagt ataaggaaca ggatgcctcg
      961 tttcatggtc tctcctccac gcctgggcgc aggtttagaa ttcgatgctg agttgggtat
     1021 cccccttggc cgggcccagt gcaatccgga tctctccctc cactacactc cagggcacgg
     1081 gcttgcccga gagcaggacc tgctttggcc gatccccggc gcgcagccag agctggttct
     1141 cctcaagggc gttggccttg aggtcgaaaa ggacgctctt tttcattttt cgggccgcag
     1201 cgatggccat cgtcgcccgg gtcacctcct gaagcggagc cgggaggtgc gccagtacga
     1261 tctggcggtc cgaggtggca tgcccgctga gccgggttcc ctgcagccgg tcccgggact
     1321 gagcgtcggc gttgaggagc agcaagtccg tcgccgcctc aaagcggaaa cgggccctgt
     1381 tgcgggttcg aacctcgacg atcagctcat cacccgaacg ccggcatgag ggcatgattt
     1441 gcgagatata ggcgaggtgg ggctggagtg caggccggtc gcccaggcgg atttcgaagg
     1501 agtgcttccc cctgctcagg ttgggcagga tgatgaggcg gtcgctgaag gcatcgtgta
     1561 tgcggccgtc gagcaggacc tcctgaatga actggtcaca gttatcgata gcgatgccca
     1621 taccgccgct gaaaccgggc atggtgtcaa gattgacccg ggtgaggtcc agggtaagaa
     1681 agagcttgtc tttctcggcc cgccagctgt aatcccactg cgccatcagc cgcagtt
//
LOCUS       DAOPWU010000544         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_76163_length_1737_cov_4.154578, whole genome shotgun sequence.
ACCESSION   DAOPWU010000544 DAOPWU010000000
VERSION     DAOPWU010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76163_length_1737_cov_4.154578"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /locus_tag="PKM23_16085"
     CDS             complement(<1..1705)
                     /locus_tag="PKM23_16085"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HNY93043.1"
                     /translation="MRNPILFFLLLITLLPAAVQPMEAVLFDGQNAAETYRLAALTLA
                     GIVNREGARLYLANVREAWSYTGTDESWEAIYRDRGGVNFNRITSFAELVDHFRSHIR
                     GVITYEPRRWGNFSDQSFFWQGEYAALIGGLTGCLPLPETTARALGFLPVDSIRVEDV
                     FDGDAPVWVPARLERPSLPWNNTSWSEEKRYLTLVDWGLRTLLPLCNPNKCYIREITD
                     FTILHRMFQLNLAGDPPGGVRFDALPAARADLIERLLTHMQNRNPGRIFHCYGWMDPE
                     PLVQWIATFGGAFHETLQNNLSWHAAFPLAPQPLQPPAQKRPDEVRLEDKYYLLFISS
                     EGDASNWALTLQAGAWLSPRRGRVPLTWGWNLHLLDLCPFIARYYYETATANDGFVSV
                     MSPLGYVYPDLWGEEVWPDAVAQTRRLMEQFGVRDFYAYKHYAPNGRIFFRGVDLYNS
                     FDFYRLGRFLGDVNAELTLLFDPQLANQYPTTRYGALLYNHVDDGTFYGQAGDLDAMA
                     RRILAGLRGKSRPGFLLAGYQRFFHDQHGSWPAAGADLSVERLEQVVQKLQADPEMGP
                     LI"
BASE COUNT          335 a          476 c          631 g          295 t
ORIGIN      
        1 cgatcaacgg tcccatttcc gggtcggcct gcagtttctg caccacctgc tcaagccgtt
       61 ccaccgagag atcggcgcct gcggccggcc agctgccgtg ctggtcatga aagaagcgct
      121 ggtagccggc gaggagaaag ccgggtctgg atttgcccct gaggccggcg aggatgcgcc
      181 tggccatggc gtcgaggtcg ccggcctgtc cgtaaaaggt gccgtcgtcg acatgattgt
      241 acagcagggc gccgtagcgg gtggtcggat actggttggc cagctgcgga tcgaaaagca
      301 gggtgagctc ggcgttgaca tccccgagaa aacgcccgag ccggtaaaaa tcaaagctgt
      361 tgtagagatc gacgcccctg aagaaaatgc ggccgttggg agcgtagtgc ttgtaggcgt
      421 agaaatcgcg gacgccgaat tgctccatca gccgccgggt ctgggcgacc gcgtccggcc
      481 aaacctcctc cccccagaga tcgggataga cataaccgag cggggacatc acgctgacaa
      541 agccgtcgtt ggccgtggcg gtctcgtagt aatagcgggc gatgaagggg cagaggtcga
      601 ggaggtgcag gttccagccc caggtgaggg gcacgcggcc gcgccgcggt gaaagccagg
      661 ccccggcttg cagggtgagg gcccagttgg aggcatcgcc ctccgagctg atgaagagga
      721 ggtaatattt gtcctcgagc cggacctcgt ccggcctctt ttgcgccggg ggctgcaggg
      781 gctgcggggc cagcgggaag gcggcatgcc acgagagatt gttttgcagg gtttcgtgaa
      841 aggcgccgcc gaaggtggcg atccactgca ccaggggttc gggatccatc cagccataac
      901 agtggaaaat gcggccggga ttccggtttt gcatatgggt gagcaggcgt tcgatgagat
      961 cggcgcgggc ggcgggcagg gcgtcgaagc ggaccccgcc ggggggatcc ccggccaggt
     1021 tgagctggaa catgcggtgc aggatggtga aatcggtgat ctcgcggata tagcacttgt
     1081 tggggttgca gagaggcagg agggtgcgca ggccccagtc gacgagggtc aggtagcgtt
     1141 tctcttcact ccaggaggtg ttgttccagg gcaggctggg ccgttcaagc cgggcgggca
     1201 cccacaccgg cgcatcgccg tcgaaaacat cctccacccg gatggaatcg acaggaagga
     1261 aaccgagggc gcgggcggtc gtttccggca gggggaggca gccggtcaat cccccgatca
     1321 gggcggcgta ctctccctgc cagaaaaagg attggtcgga aaagttgccc cagcgccgcg
     1381 gctcataggt gatgacgccg cgaatatggc tgcgaaagtg atcgaccagc tcggcgaagc
     1441 tggtgatgcg attgaaattc accccgcccc gatcccggta gatggcctcc cagctctcgt
     1501 cggtcccggt atagctccag gcctcgcgga cattggcgag gtagagccgg gcgccctcac
     1561 ggttgacgat gccggcgagg gtcagggcgg cgaggcggta ggtttcggcc gcattctggc
     1621 catcgaacag tacggcttcc atcggctgga ccgctgccgg gaggagggtg atcaggagga
     1681 gaaaaaagag tatgggattg cgcatgggtt ctccgccgga gggttggatg aataatt
//
LOCUS       DAOPWU010000545         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_76771_length_1728_cov_4.994023, whole genome shotgun sequence.
ACCESSION   DAOPWU010000545 DAOPWU010000000
VERSION     DAOPWU010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76771_length_1728_cov_4.994023"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            484..>1728
                     /locus_tag="PKM23_16090"
     CDS             484..>1728
                     /locus_tag="PKM23_16090"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HNY93044.1"
                     /translation="MRVSCCYLLLVFLLLLSSFAPRSAGAGEWKKGNPPLATRWFSEA
                     GPEQARPEYPNPAMAREAWMNLNGLWRYAIRPRGEQLLDQPGPAPAAGWEGEILVPWP
                     VESALSGVQRPVSPEQRLWYSRTFTVPAEWRKKKILLHFEAVDWQSQVWVNGIEVGYH
                     EGGYDAFSFDITPALRFAGDEVVTVAVWDPTDTGTQPRGKQVLKPGGIFYTACTGIWG
                     TVWVEPVPVTRIEELRIVPDVAAGAVTVTCTVAGALPRSRLALTALEEGRIVASASGA
                     ADAPIRLAIPGARLWSPATPHLYDLKVQLIDREKVTDEVSSYFGLRSITVGPDQAGVT
                     RLLLNGEPLFQAGPLDQGFWPDGIYTAPTEEAMVYDLEVLKRMGFNMLRKHVKVEPRR
                     FYYHCDRLGVLVWQDMPSGDQSL"
BASE COUNT          295 a          552 c          520 g          361 t
ORIGIN      
        1 gatctgggga ctgttcaagg ctgatttgtt gcccataccg agggcgatga cctcgagctg
       61 ctgcccctcc ccgcagagga ccggcaggct ccccacctgg gcctctccgg ccagagccca
      121 aaccagcagc agggccgcag cacccagaat gacgatgtgt ctggttctgt tcattttttt
      181 cctctccccg ggtcaaaatg ggcgcgcgca ggcgggctgc ctgcaacaaa tcagcgtcag
      241 ttttgtccct tttctttgac agtgccatga attgtatttc tattatccca ttaaaataca
      301 attcattatg gattgaactc agtgctcctc gcacctcatt ttacaataat caggccagag
      361 tcgaaaattc tggcttttta tatccaggtt gtttattttt atacggctcc ggacgggagt
      421 gcgcagtggc gcacccgccg accggggcag cggtccggct atcatttgcc gagaggagaa
      481 gcgatgagag tttcctgctg ctatctgctg ctggtgtttc ttctgctgct gagctccttc
      541 gccccgcgga gtgccggggc cggggagtgg aaaaagggaa atccgccttt ggcgacgcgg
      601 tggttcagcg aggcgggtcc ggagcaggcc cgtcccgagt accccaatcc ggccatggcc
      661 cgcgaggcct ggatgaatct caacggtctc tggcgttacg ccatccggcc gcggggggag
      721 cagctcctgg accagcctgg gccagcgccg gctgcaggat gggaagggga gattctcgtc
      781 ccctggccgg tcgaatcggc cctctccggg gtgcagcgtc cggtctcacc cgagcagcgc
      841 ctctggtaca gccgcacctt cacggttcct gcagaatggc gcaaaaagaa gatccttctc
      901 cacttcgagg cagtcgactg gcagagtcag gtctgggtga acggcatcga ggtcggctat
      961 catgaaggcg gctatgatgc cttctctttt gatatcaccc ccgccctcag gttcgccggt
     1021 gatgaggtgg tcaccgtggc cgtctgggat cccactgaca ccggcaccca gccccgcggc
     1081 aagcaggtcc tcaagcccgg cggcattttt tacaccgcct gtaccggcat ctggggcacg
     1141 gtctgggttg aaccggtgcc tgtgacccgc atcgaggagt tgcgcattgt gcccgatgtc
     1201 gccgccggcg ccgtcaccgt gacctgcacg gttgccgggg ccttgccccg cagccgcctc
     1261 gccctcaccg cgctggaaga gggccggatc gtcgcctccg cgagcggagc cgccgatgcc
     1321 cccatccggc tggccatccc cggggccagg ctctggtcgc cggccacacc ccacctctat
     1381 gacctcaagg tccagctcat cgaccgggag aaggtgaccg acgaggtcag cagctatttt
     1441 ggcctgcgct ccatcactgt cggtcctgat caggcgggtg tcaccaggct gttgctcaac
     1501 ggcgagcctc tcttccaggc cggacccctt gatcagggct tttggcccga cggcatttat
     1561 accgcgccga ccgaagaggc catggtctac gacctcgagg tgctcaagcg gatgggcttt
     1621 aatatgctgc gcaagcatgt caaggtcgaa ccgcgccggt tctactatca ttgcgatcgc
     1681 ctcggcgtgc tcgtctggca ggatatgccc agcggcgatc agtccctc
//
LOCUS       DAOPWU010000546         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_77517_length_1718_cov_3.695731, whole genome shotgun sequence.
ACCESSION   DAOPWU010000546 DAOPWU010000000
VERSION     DAOPWU010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77517_length_1718_cov_3.695731"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..>1718
                     /locus_tag="PKM23_16095"
     CDS             444..>1718
                     /locus_tag="PKM23_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNY93045.1"
                     /translation="MDVFDFRKRLIRDYRDYVESFIQIKDPRIWDHVHKVLDEGELWP
                     ESLIQLNPAFAHGAWIDELVQNNTLHPLCQRIFRRKNDPADLGKPLRLHLHQTQAIDT
                     AKTGASYVLTTGTGSGKSLAYIIPIVDAVLRNGPGKGIKAIIIYPMNALANSQCGELE
                     KFIQWGFAKDQYPLRFAKYTGQENEDQRKAIIQNPPDILLTNYVMLELILTRPEERGL
                     IQAARNLQFLVLDELHSYRGRQGADVSLLLRRVRETLGAWQMQCVGTSATLAGEGTFA
                     EQQREVAKVASQLFGTEVKPEHVIGETLQRLTPELDFSQQAACTSLVQQANAEPDFPP
                     EGSQFIRQPLSSWLESTFGVTTDPHSGRLVRARPCAIGGEEGAAQRLANLTGLTASHC
                     ARVIKEALLAGIRCTPLPDSAIPPFVYRLHQFI"
BASE COUNT          399 a          524 c          422 g          373 t
ORIGIN      
        1 acactggcct gtcaagccag aggtcgcggg ttcgagcccc gtcggccccg ccaaggtaaa
       61 agcagcaaaa acatgaagaa gccctcattt ctgagggctt ttttattggc cggaattctc
      121 atttttaccc atctttggac acaaaatcac ccccgctgta accccctcga actctagggc
      181 atggctgtta taattcgata actagttgtt gtcatcagat gtatgctcac tgtaaaatcc
      241 agaatgccta aaaatcgata aagaatgtaa tttcaacctg gctccccgtt tcccttgact
      301 ttatccaata acttgctaaa ttaaagctaa acaacttggt gatgggaatt cggggcctac
      361 caaatatccg ctctatccca ccgtcgtgat ccagttgatc ctgttttgcg tatgtactga
      421 atctctccag gcaggactca cttatggatg ttttcgactt ccgcaaacgg ctgatcagag
      481 actatcggga ttacgtagaa agctttattc aaatcaagga cccgcgcatt tgggaccatg
      541 tccacaaggt cctcgacgaa ggcgagctct ggcccgaatc cctgattcag ctcaatcccg
      601 ccttcgcaca cggcgcctgg atcgatgagc tggtccagaa caacacgctc cacccgctct
      661 gccaaagaat atttcgccgc aagaatgatc ccgccgattt gggcaaaccc cttcgtctcc
      721 accttcatca gacacaagcc attgataccg cgaaaaccgg cgcctcctat gtcctcacca
      781 ccggaacggg ctctggaaaa agcctcgctt atatcattcc catcgtcgat gcggtcctgc
      841 gcaatggccc gggcaaaggt attaaagcaa ttattatcta tcccatgaat gctttggcca
      901 acagccaatg cggcgaactg gaaaaattta tccagtgggg ttttgcaaaa gatcaatatc
      961 cccttcgttt cgccaaatac accggccagg aaaatgagga ccagcgcaag gcgatcatcc
     1021 agaatccccc ggacattctc ctgaccaact atgtgatgct cgagctgatc ctcacccgcc
     1081 ccgaagagag gggacttatt caggccgcgc gcaatctgca gtttctggtg ctcgatgagc
     1141 tgcactccta tcgcggccgt cagggcgccg atgtcagtct gcttctccgc cgtgtgcgcg
     1201 aaactctcgg cgcctggcag atgcagtgcg tcggcacctc agccacactg gcgggcgagg
     1261 gcacgttcgc ggagcagcag cgcgaggtcg caaaagtcgc cagccagctt ttcggcaccg
     1321 aggtcaaacc ggaacacgtg attggcgaaa ccctgcagcg cttgactccg gagttggatt
     1381 tttcccagca agcggcctgc accagcctgg ttcaacaagc aaatgcagag cccgatttcc
     1441 cgccggaagg ttcgcagttc atccgccagc cgctttcatc ctggctcgaa tcgacctttg
     1501 gcgtgaccac cgatccccat agcggccggt tggtgcgcgc cagaccctgc gccatcggcg
     1561 gcgaggaagg cgcggcacag agattggcca acctgaccgg gctgacggca agccactgcg
     1621 ccagggtgat caaggaggcc ctgctggccg gcatccggtg tacgcccctg ccggatagcg
     1681 ccattccacc gtttgtctac cgcctgcatc aattcatc
//
LOCUS       DAOPWU010000547         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_81876_length_1659_cov_3.698878, whole genome shotgun sequence.
ACCESSION   DAOPWU010000547 DAOPWU010000000
VERSION     DAOPWU010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81876_length_1659_cov_3.698878"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            548..1078
                     /locus_tag="PKM23_16100"
     CDS             548..1078
                     /locus_tag="PKM23_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93046.1"
                     /translation="MRKSALTIILIFCLTTVLLAQQPSSIGANFAYAYRLQENPEIWS
                     KIGISLGLTGRIAVGSPILMLTAGARYVYFPEGYEQTGSGLLRHDAYDLFTPFFGLQL
                     GRATGPYLVVAETGDFGEKETRFGLDAGVGYQLWEGPARTKFDASLKYCLANLMGKDD
                     GEKTRSYLLFSMGFAF"
     gene            1168..>1659
                     /locus_tag="PKM23_16105"
     CDS             1168..>1659
                     /locus_tag="PKM23_16105"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNY93047.1"
                     /translation="MSRLEEKLKEKVFHKPDYPPGEEIVNSITHGLGVLLSLSALVIL
                     VARAARLGTTWHIVSFAVFGVTLIILYLFSTLYHSMTHRATKSVFARMDHAAIFLLIA
                     GTYTPIMLTVLRGPWGWTYFGIIWGMAILGVVIRSIYLSRFRALSTVMYAVMGWSFLI
                     AYNQ"
BASE COUNT          378 a          496 c          392 g          393 t
ORIGIN      
        1 acctggatat cagcaaaaaa tggtcaatgc cggtgcaaaa ctgggcgcta ataatttcgc
       61 atctctctat attgtacaag gagagattgc agcttaaaat ctgatcctgt tcatttacac
      121 aaaaatattt acagtctcat tatttactgc aatttcatac ctgacaacta acatttaacg
      181 ttgattaaaa gatttacttc tatgaacttt agataatcat aaacttaaat attatctcac
      241 ttcccgcccc cggcaccagc tctcgtttac aagccagcca ggccgccaac cttgctggct
      301 ttttttatac cggagatcac cgggaataga gaagagagca gtttgtttct ttttgcttga
      361 ttttggcggc gggaatgaat aatttactga aaatattgcc gctccatcgg gcaagcaaat
      421 gccggccaga gccctgatcc ttttggcgca gcctgcagag gcactgccag gggagggcag
      481 cagcagggga atccagccgc cgaattattt cacgtatgcc gttatcagcc aaccggagga
      541 atcgaagatg aggaaatcag cgctgaccat catcctgatt ttttgtctga cgaccgtcct
      601 gctcgcacag cagccgagct ccatcggcgc caatttcgcc tatgcctatc gcctccagga
      661 gaatcccgaa atctggagca agatcggcat ctcgctcggc ctcaccggca gaattgcggt
      721 cggaagtccg atcctgatgc tcaccgccgg cgcaagatat gtctattttc cggaaggcta
      781 cgaacaaacc gggtcaggcc tgctcaggca tgatgcctat gatctcttca cccccttctt
      841 cggcctccaa ctgggcaggg ccaccggccc ctatctggtc gtggccgaga ccggagattt
      901 cggagaaaaa gagacccgct tcggcctgga cgcgggcgtc ggctatcagc tctgggaggg
      961 gccggccaga accaaatttg acgcctctct caaatactgc ctcgccaacc tcatgggcaa
     1021 ggacgacggt gaaaaaacca ggagctacct cctcttctcc atgggttttg ccttttaagg
     1081 aagcgctgaa atgcacaagg gcgaagccgg tcttcgccct tttttattgc cgcctcacca
     1141 acactgaaaa cgcgaacagg attattcatg agcagactgg aagagaaact gaaagagaag
     1201 gtttttcaca aacccgatta tccccccggc gaggagatcg tcaacagcat cacccacggc
     1261 ctgggtgtgc tgctctccct ttccgccctg gtcattctgg tcgcccgcgc cgcccggctt
     1321 ggcaccacct ggcacatcgt cagctttgcc gtctttggcg tcaccctcat cattctctac
     1381 ctcttctcca ccctttacca ctcgatgacc catcgggcga ccaaaagcgt ctttgcacgc
     1441 atggaccatg ccgccatctt tctgctcatc gccggcacct acaccccgat catgctcacc
     1501 gtcctgcgcg gcccctgggg ctggacctat ttcggcatca tctgggggat ggccatcctc
     1561 ggggtggtca tccgctcgat ctacctgagc cgcttccgcg cactttcaac cgtgatgtac
     1621 gccgtgatgg gctggtcctt tctgatcgcc tacaaccag
//
LOCUS       DAOPWU010000548         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_82483_length_1651_cov_3.630326, whole genome shotgun sequence.
ACCESSION   DAOPWU010000548 DAOPWU010000000
VERSION     DAOPWU010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82483_length_1651_cov_3.630326"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1141)
                     /locus_tag="PKM23_16110"
     CDS             complement(83..1141)
                     /locus_tag="PKM23_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY93048.1"
                     /translation="MKKSAIISILLIGLALSGPLGAQEPTRIGTTAANFLEIGYGTAG
                     SAMGDAYVSMARDLSALYWNPAGLADMQKNQALFVYQPWIAGIKTSFAGVGLNLHNYG
                     TLSLGFTSVNYGDMKVTTMAMQEGTGELFSASDMAFSLAYARKLTNWFSFGGAAKYIN
                     SSIWHTDASAMAIDLGVIINTYFFSPNGRREQGLHIGMSISNYGSRLRYDGIDLINPI
                     DILADENGNFSDVPGRFHLSEWELPLIFRLGASLTPFYTDHHEMILSLDAIHPNNNNE
                     SINLGAQYAFTSAGFGRLFLRGGYKALFMNRSEFGPTFGAGVETFLLGNAGVKIDYAW
                     RDVGLLGNINTFGVMVMF"
     gene            complement(1258..>1651)
                     /locus_tag="PKM23_16115"
     CDS             complement(1258..>1651)
                     /locus_tag="PKM23_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93049.1"
                     /translation="PFSPKDSLMFTTRPEVAVDVKELASSLDDILVVPNPYVATNAME
                     TAISNPFLNQRRKLMFTHLPAQCTIKIFTASGIFVDEIQVDNPPERGIVHWDMLTREG
                     LEIAAGIYVYHVKTPTGEEKLDKFAVIK"
BASE COUNT          326 a          424 c          565 g          336 t
ORIGIN      
        1 agctccgttt tctctgcgtt ctctgtgcca ccgtgtgaga attttccatg cgtgctctgc
       61 ggcgctgcgc gagatcattt ctttaaaaca tcaccatcac cccgaaggtg ttaatattgc
      121 cgagcagacc gacgtcccgc caggcgtaat cgatcttgac gccggcattg ccgagcaaaa
      181 aggtctcgac accggcgccg aaggtcgggc cgaactcgga gcggttcatg aagagcgcct
      241 tgtagccgcc gcgcagaaaa agacgtccga acccggccga ggtgaaggcg tactgggcgc
      301 cgaggttgat cgactcgttg ttgttattgg gatggatggc atccagcgag aggatcatct
      361 cgtgatggtc ggtgtaaaat ggcgtcaggg aggcgccgag gcggaagatc aggggcagtt
      421 cccattcgga gagatggaag cggccgggca cgtcgctgaa attgccattc tcgtccgcga
      481 ggatatcgat cggattgatg aggtcgatgc cgtcgtagcg caggcgggag ccgtaattgg
      541 agatgctcat gccgatatgc agcccctgtt cgcgtcggcc attgggcgag aagaagtagg
      601 tattgatgat cacgcccaga tcgatggcca tggcggaggc gtcggtgtgc cagatcgagg
      661 agttgatata tttggcggcg ccgccgaagg agaaccagtt ggtcagcttg cgcgcatagg
      721 cgaggctgaa ggccatatcg ctggcggaga aaagctcgcc ggttccctcc tgcatggcca
      781 tggtggtcac cttcatgtcg ccatagttga cgctggtgaa gccgagggag agggtgccgt
      841 aattatgcag attgagtccc acgccggcga aggaggtctt gatgccggcg atccagggct
      901 ggtagacaaa gagggcctgg tttttctgca tatccgccag tccggcgggg ttccagtaga
      961 gggccgaaag atccctggcc atgctcacat aggcgtcgcc catggccgag ccggcagtgc
     1021 cgtagccgat ttcgagaaaa ttggcggcgg tggtgccgat gcgggtgggc tcctgcgcac
     1081 ccagcgggcc ggagagggcc agtccgatga gcaggatgga tataatggca ctttttttca
     1141 tgctcgaaac ctcagtttgt cattcccggt cgcatgccgc agccggccct cacaagggac
     1201 gggtgcggcg gtcacagatc agggggcact ccggccgcaa gagcggccgg gagatgctca
     1261 cttgatcacg gcaaatttat ccagcttctc ctcgccggtc ggggtcttga cgtggtagac
     1321 gtagatcccg gcggcgatct ccagcccctc gcgggtgagc atgtcccagt ggacaatgcc
     1381 gcgctcgggg ggattgtcga cctggatctc gtcgacaaag atgccgctgg cggtgaagat
     1441 cttgatcgtg cactgcgccg ggagatgggt gaacatcagt ttgcggcgct gattgaggaa
     1501 gggattggag atggcggtct ccatggcgtt ggtggcgaca tagggattgg ggaccaccag
     1561 aatgtcgtcc agcgaggagg cgagttcctt tacatctacc gctacttccg gccgggtggt
     1621 gaacatgagt gagtctttgg gggagaaagg g
//
LOCUS       DAOPWU010000549         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_82795_length_1647_cov_4.103643, whole genome shotgun sequence.
ACCESSION   DAOPWU010000549 DAOPWU010000000
VERSION     DAOPWU010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82795_length_1647_cov_4.103643"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1357)
                     /locus_tag="PKM23_16120"
     CDS             complement(134..1357)
                     /locus_tag="PKM23_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNY93050.1"
                     /translation="MEYNRPKYDLFEILFIIFLALLLALFFYAMRLTLIPPLLALILI
                     AILAPLKNPVARSLLIVVIVVFLFWFIRDTLDILTPFAIAFGLAYLFDPLVTRLERRN
                     VPRLLSVSIIVFLSLGVFILVMILSVPRIISELGNLVSFVMGLPPRVGEWINSGGAAM
                     FGDTQEEVAKIQEILNRELPHRLAKVSDLLIQKAVQFTQGLPHIFPKLLYLILTPFLF
                     FYLLKDFHKIRRWILELLPIESSWVVREYVEKVDSIISGFFRGQFIVCTLVGILTTLL
                     LLLFRVEYALLIGIMAGALNIIPYVGLAITLFVGLLTAIASPDPLMTSLKVIIAVESI
                     RILENSVLSPRIVGNRVGLHPVWVIFSILICAHFLGFVGLILAVPLAASLKIFVSVGM
                     HSYRRKIWRRKRNEN"
     gene            complement(1362..>1647)
                     /locus_tag="PKM23_16125"
     CDS             complement(1362..>1647)
                     /locus_tag="PKM23_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93051.1"
                     /translation="CKVYDGKVEVGPVTKWSTSMPRETRSGPPQQVQGPVEVPGPFEV
                     TLEEWQQIVRGQQIVVRKDGKFAKSLFDQRADSTDEWVKWNKELDAKAKK"
BASE COUNT          416 a          431 c          496 g          304 t
ORIGIN      
        1 caggcagtag ctctcctcgg ctgagtagaa ctggaaatcg atggaatcgc gtatctcatg
       61 gctgtcgatg gcgatgatct catcgcccgg ttgcaggcca agcctggccg ccggtgtatc
      121 cggataaacg acgctaattt tcattgcgct tgcgtcgcca gatcttgcgc cggtaggaat
      181 gcatgcccac agagacgaaa atcttcaatg aggcggcgag cggaacagcg aggatgaggc
      241 cgacaaagcc gagaaaatgg gcacagatga ggatcgaaaa gatcacccat accggatgca
      301 gaccgacacg gttgccgacg atgcgggggg agagcaccga attctcgaga atgcggatgc
      361 tctcaacggc gatgatgacc ttgaggctgg tcataagcgg atcaggactg gcaatggcgg
      421 tgagcagacc gacaaaaagg gtgatggcca ggccaacata ggggatgatg ttaagggcac
      481 cggccataat gccaatcaac agggcatact cgacgcggaa gagcaggaga agcaatgtgg
      541 tcagaatgcc caccagggtg cagacgatga actgtcctcg gaaaaagccc gagatgatag
      601 aatccacttt ttccacgtac tcacgaacca cccagctgct ttcgataggc agcagctcga
      661 gaatccagcg ccggatcttg tgaaaatcct tgagcagata gaagaagaga aagggcgtca
      721 ggatcagata gagcagcttg ggaaagatgt gcggcaatcc ctgggtgaac tggaccgcct
      781 tctggatgag gagatccgaa accttggcga ggcggtgggg cagctctcgg ttgaggatct
      841 cctggatttt ggccacctcc tcctgggtat cgccgaacat cgcagccccg ccgctgttga
      901 tccattcacc cacacgcggg ggcagtccca tcacgaaaga gaccagattg cccagttcac
      961 tgatgatcct cggcaccgag aggatcatga ccaggataaa aacgccaagg ctgagaaaga
     1021 cgatgatgct aacgctgagc aggcgcggca cgttcctgcg ctccagcctg gtgaccagag
     1081 ggtcaaaaag ataggccaga ccgaaagcga tggcaaaggg agtcagaata tccagggtat
     1141 cacggatgaa ccagaagaga aagaccacga tcaccacaat aagcaggctt ctggccaccg
     1201 ggtttttaag cggagccagg atggcgatga gaatgagtgc cagcagcggc gggatcaggg
     1261 tcaggcgcat cgcgtaaaag aacaaggcca gcagcagggc cagaaaaatt ataaatagga
     1321 tttcgaagag gtcgtatttt ggtcggttgt attccatcgc gctacttttt ggcctttgcg
     1381 tcgagttcct tgttccactt cacccattca tcggtcgaat ccgccctttg atcgaagagc
     1441 gatttggcga atttgccatc cttgcggacg acgatctgct gcccgcgcac gatctgctgc
     1501 cactcctcca gggtcacctc gaagggaccg ggcacctcca cggggccctg tacctgctga
     1561 gggggaccgc tgcgtgtctc cctgggcatg gaggtgctcc acttggtgac cgggccgacc
     1621 tcgaccttgc cgtcgtagac cttgcag
//
LOCUS       DAOPWU010000550         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_84676_length_1623_cov_4.698342, whole genome shotgun sequence.
ACCESSION   DAOPWU010000550 DAOPWU010000000
VERSION     DAOPWU010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84676_length_1623_cov_4.698342"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKM23_16130"
     CDS             <1..339
                     /locus_tag="PKM23_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93052.1"
                     /translation="VVPNPYVVTNVMEPAIGNWERNQPRRLMFTNVPAQCTIKIFTVS
                     GLLVDEIEVNNAVGARKNPFDSNSESNGTAFWDLQSKEGLDIAAGYYLYRIESTLTGK
                     VKMGKFAVIK"
     gene            431..>1623
                     /locus_tag="PKM23_16135"
     CDS             431..>1623
                     /locus_tag="PKM23_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93053.1"
                     /translation="MKRYTIIPLLAAVLFAGCSRERNLERPEKILARVGGKSITVNEF
                     IRRAEYTPRPPYCSSESYPHKKIVLNSLIAEKLFALEAGEKNPLITSPRVEAFLKGRK
                     EQAMREYLFIKEAYAKAQIDTAVLKQTVVNASRRYRVAYFNVPDSATAVEVEEGLRKR
                     REPMLAAMNSFAPLDSLPEREVVWDKTESDGVIDSLFSVRRQAGDLIGPIKNAQGHSL
                     FMQVLEVKPEVVVTNEQLREIWKSVKERLTDKEARANWDQYILSVMKGRRIEFNRSTF
                     FKLADLLGPLYLKSRDEQEASFEQRYWEGTGEDVNWDDYLSQLKAMAGEPLFKVDGEI
                     WDVARVAEEIRSHPLVFREKKIRNRDFGRQLQLAIIDMVRDRYLTERAYKRGYDRLGA
                     VEREVG"
BASE COUNT          358 a          507 c          472 g          286 t
ORIGIN      
        1 gtggtgccca atccctatgt ggtcaccaac gtgatggagc ccgccatcgg caactgggag
       61 cgcaatcagc cccgccggct gatgttcacc aatgtgccgg cccagtgcac gatcaagatc
      121 ttcaccgttt ccggcctgct cgtcgacgaa atcgaggtca acaatgccgt cggcgcccgc
      181 aagaacccct tcgattccaa cagcgaatcc aacggcaccg ccttctggga tctgcagagc
      241 aaggagggac tcgacatcgc cgccggttat tatctctacc gtatcgaatc cacgctgacc
      301 ggcaaagtca agatgggcaa gttcgccgtg atcaaataat ccacgggctc atcccgcaag
      361 ggtgcagcct gacatcaact ggatgggccc tgcctgcacg gcagggccca tctctattta
      421 cggatttaca atgaaacgtt atacgatcat accgctcctc gccgctgtcc tgttcgccgg
      481 ctgcagccgg gagcgcaatc tggaaagacc ggaaaagatc ctggcgagag ttggcggcaa
      541 gagcatcact gtcaatgagt tcattcgccg cgctgaatac acgccgcgcc cgccctactg
      601 cagcagtgag agctatcccc acaaaaagat cgtcctcaac tccctcatcg ccgaaaagct
      661 cttcgccctc gaggccgggg aaaagaatcc cctcatcacc tcaccccgag tcgaggcttt
      721 cctcaaggga cgcaaggaac aggcgatgcg cgagtacctg tttatcaagg aagcctacgc
      781 caaggcgcag atcgacacgg cagtgctgaa acagacggtg gtcaacgcct cccggcggta
      841 tcgcgtcgcc tacttcaacg tcccggacag cgccacagcc gtggaggttg aggaggggct
      901 gcgcaagcgg cgcgagccga tgctggctgc gatgaactcc ttcgcccccc tcgactcact
      961 gcccgaacgc gaggtggtct gggacaaaac cgagagcgac ggggttatcg actccctctt
     1021 cagcgtccgc cggcaggcgg gagacctcat cggcccgatc aaaaatgcac aagggcactc
     1081 tctcttcatg caggtgctcg aggtcaagcc cgaggtcgtg gtcaccaacg agcagctgcg
     1141 cgagatctgg aaatcggtca aggagcgctt gaccgacaaa gaggcgcgcg cgaactggga
     1201 ccagtacatc ttgtccgtca tgaagggccg tcgcatcgaa ttcaaccgct cgaccttttt
     1261 caaactcgcc gatctccttg gcccgctcta tctcaagagc cgggacgagc aggaggcctc
     1321 tttcgagcag cgctactggg aggggaccgg tgaggatgtc aactgggatg attaccttag
     1381 tcagctcaaa gccatggccg gcgagcccct cttcaaggtg gacggcgaga tatgggatgt
     1441 ggcgcgcgtt gccgaggaga tccgcagcca ccccctggtc tttcgtgaga aaaaaatcag
     1501 aaaccgggat ttcggccggc agctgcagct ggcgatcatc gacatggtcc gcgaccgcta
     1561 tctcacggag cgcgcctaca agcgcgggta tgaccgcctc ggggcggtcg agcgcgaggt
     1621 ggg
//
LOCUS       DAOPWU010000551         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_86386_length_1603_cov_4.887597, whole genome shotgun sequence.
ACCESSION   DAOPWU010000551 DAOPWU010000000
VERSION     DAOPWU010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86386_length_1603_cov_4.887597"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PKM23_16140"
     CDS             complement(<1..448)
                     /locus_tag="PKM23_16140"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNY93054.1"
                     /translation="MAFYTLILIAIGLSMDALAVSISSGLSIPEVNRSHGLRIGFSFG
                     LFQAVMPVIGWLAGTTLRVYIERVDHWIAFCLLALVGARMIYEALTGHDGHSPVNASE
                     PKVLLLLSLATSIDAMAVGLSFALLHVPLFPAVLIIGVTTFLFSGAG"
     gene            complement(518..1168)
                     /locus_tag="PKM23_16145"
     CDS             complement(518..1168)
                     /locus_tag="PKM23_16145"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNY93055.1"
                     /translation="MIPIRDDNARHHFPVVTVTLIAANVIVFIYQSLLPSLQVSEFIL
                     EYGAVPYYITNGSHLGTIFSSMFLHGGFMHLGGNMLYLFIFGDNIESVLGSLRYLLFY
                     LLCGVAAFASHYLFDLSSQIPMIGASGAISGVLGAYAVRFPHAKVSVLLPLIIIWQVI
                     TVPAVLVLGFWFVMQLFSGATGSAQSGGVAWLAHVGGFVAGVLFMLVWKRRHNLGF"
     gene            complement(1181..>1603)
                     /locus_tag="PKM23_16150"
     CDS             complement(1181..>1603)
                     /locus_tag="PKM23_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93056.1"
                     /translation="PGQVALYRRAPRDQWKYIGRESGAHGAIGSRSRELGEFALIRDS
                     EAPVITAVIPAAGARLSQRTPLLRAGVRDALSGIGDDEPYALILDGERLITEYDPEGG
                     RLLHQVESPLSAGRHTLEVVVRDRCGNEARTSSTFHVN"
BASE COUNT          342 a          466 c          556 g          239 t
ORIGIN      
        1 ccccggcccc cgagaaaaga aaggtggtga cgccgatgat cagcaccgcc ggaaagaggg
       61 gcacgtgcag gagggcgaag ctgagcccca cggccatggc gtcgatgctg gtggccagag
      121 agagtaggag aagcactttg ggctcggagg cgttgaccgg ggagtgccca tcgtggccgg
      181 tgagggcctc gtagatcatg cgcgcgccga ccagggcgag caggcaaaag gcgatccagt
      241 ggtcgacccg ctcgatataa acgcgcaggg tggtgccggc aagccagccg atgacgggca
      301 tcaccgcctg aaagaggccg aaggaaaaac cgatcctgag cccgtgcgag cgattgacct
      361 ccgggatgct cagtccgctg gagatcgaga ccgccagggc gtccatggag aggccgatgg
      421 cgatgaggat gagggtataa aaagccaagt tcgctaccgg ttatacgcct gcctatgacg
      481 gcgggcggat gtgatgatga tttcagggat gctgtggtca gaatcccagg ttgtgccggc
      541 gtttccagac cagcatgaac agaacccccg cgacaaatcc cccgacatga gccagccagg
      601 ccaccccccc gctctgtgct gaacccgtcg ccccgctgaa aagctgcatg acgaaccaga
      661 aacccagcac cagcaccgcg ggaacggtga tgacctgcca gatgatgatc agcggcagca
      721 ggaccgacac ctttgcgtgg ggaaagcgca ccgcgtaggc cccgagcacc ccggagatgg
      781 cgccgctggc gccgatcatc ggaatctgcg aagagagatc gaagagatag tgggaggcga
      841 aagccgccac cccgcagagc agatagaaga gcaggtagcg cagcgagccc agcaccgatt
      901 caatgttgtc gccgaagatg aagagataga gcatgttgcc gccgagatgc ataaagcccc
      961 cgtgcaggaa catcgagctg aagatggtgc cgagatggct gccgttggtg atgtagtagg
     1021 gtacggctcc gtactcgagg atgaactcgc tgacctggag tgaaggcagc aggctctgat
     1081 agataaagac gatcacattg gccgcgatca gggtaacggt cacgaccggg aagtggtggc
     1141 gggcattgtc atcccgaatg ggtatcatgg ttatcccccc tcaattgaca tgaaaggtgc
     1201 tgctggtgcg ggcctcattg ccgcagcggt cacggacgac aacctccagg gtatggcggc
     1261 cggcgctgag gggcgactcg acctggtgga ggagacgccc cccttcagga tcatattccg
     1321 tgatcaggcg ctcgccgtca aggatgaggg cgtagggttc gtcgtcgccg atgccggaga
     1381 gggcgtcgcg cactccggcc cggagcagcg gcgtgcgctg gctcaggcgc gcgcccgcgg
     1441 ccgggatcac ggcggtgatc accggcgcct cgctgtcgcg gatgagggcg aattcgccca
     1501 gctcacggct gcggctgccg atggcgccgt gcgctccgga ctccctgccg atgtacttcc
     1561 actggtcgcg cggcgcacgg cggtagagcg ccacctggcc ggg
//
LOCUS       DAOPWU010000552         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_89085_length_1572_cov_3.334871, whole genome shotgun sequence.
ACCESSION   DAOPWU010000552 DAOPWU010000000
VERSION     DAOPWU010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89085_length_1572_cov_3.334871"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PKM23_16155"
     CDS             complement(<1..724)
                     /locus_tag="PKM23_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY93057.1"
                     /translation="MKFTRREFIQTAALSGAGIMLTGAETLRAQGERKKRYAIVGTGS
                     RSYMYQEAVLKTFAGSCGMTGYCDTNRGRLERAQRFALSLGQPAPPIYAAADFDRMIS
                     ETRPDVVIVTTVDGFHHQYIVRALELGCDVITEKPMTTDDARCRAILKAVRRSGRKCT
                     TAFNYRYSPPRTQVKELLMQGAIGDILSVDFHWMLNTHHGTDYFRRWHSQKKNSGGLM
                     VHKATHHFDLINWWLSAVPVSVF"
     gene            complement(847..>1572)
                     /locus_tag="PKM23_16160"
     CDS             complement(847..>1572)
                     /locus_tag="PKM23_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNY93058.1"
                     /translation="GVSWWYNWAITPSTSAPADYQTDYAMDFVPMLWGGNTSAADITR
                     VKAFVISHPEVQHLLVMNEPNLTNQANRTPLQAATDWLKYEQVIQELAGLGRTVYLVG
                     PAITWGTQSGYADPVSWLDAFYDAYKTANSGREPQIDYLAFHWYDYGLNAQLDRLKKY
                     NKKIWVTEMANWNAQINSYAKQKQQMTEMVTICENRADVYRYAWFYGRGDYPDRHFTY
                     LFTPNDGELSELGRLYIELPFAQ"
BASE COUNT          261 a          444 c          515 g          352 t
ORIGIN      
        1 cgaagaccga gaccgggacc gcagatagcc accagttgat gaggtcaaaa tggtgggtgg
       61 ccttgtgcac catcagtccg ccggaattct ttttctggct gtgccagcgg cggaaatagt
      121 cggtgccgtg atgggtattg agcatccagt gaaaatcgac cgagaggatg tcgccgatgg
      181 ccccctgcat gagcagctcc ttgacctggg tgcgcggcgg tgagtagcgg taattgaagg
      241 cggtcgtgca tttcctgccg ctgcggcgca ccgccttgag gatggcgcgg cacctggcgt
      301 cgtcggtggt catcggcttt tcggtgatga cgtcgcagcc cagttcgagg gcgcggacga
      361 tgtactggtg atgaaagccg tccaccgtcg tgacgatgac cacatccggc cgggtctctg
      421 agatcatgcg gtcgaaatcg gcggcggcgt agattggcgg tgccggctgg ccgagggaga
      481 gggcgaagcg ctgcgccctc tcaaggcggc cacggttggt gtcgcagtag ccggtcatcc
      541 cgcagctgcc ggcaaaggtt ttcagtaccg cctcctggta catataggag cgcgagccgg
      601 tgccaacgat ggcgtagcgt tttttgcgct ccccctgcgc acgcagggtc tccgcccccg
      661 tcagcatgat cccggctcca ctcagggcgg cggtctggat gaattcgcgg cgtgtgaatt
      721 tcatcggcat gctctccttg ttggatcctc tctctgcccg gctcctgaca cggccggcgc
      781 acttttttcc gatcggcagg tctcgttcta ttggtccgcc ttcttttccg gaaggccggt
      841 gaaaaactat tgggcgaagg gcagctcgat gtagagcctg cccagttccg acagctcgcc
      901 gtcgttgggc gtgaaaaggt aggtgaagtg gcggtccggg taatcgcccc ggccgtagaa
      961 ccaggcatag cggtagacat ccgcccggtt ctcgcagatc gtcaccatct cggtcatctg
     1021 ctgcttctgt ttggcgtagg agttgatctg tgcgttccag ttggccatct cggtgaccca
     1081 gattttttta ttatattttt tcaaccggtc cagctgcgca ttcaggccgt aatcatacca
     1141 gtgaaaggcg agatagtcga tctgcggttc cctgccgctg ttggcggttt tgtaagcgtc
     1201 gtaaaaggcg tcgagccagc tcacggggtc cgcgtatccg gactgggtgc cccaggtgat
     1261 ggccggaccg acgagataga ccgtccggcc gaggccggcc agttcctgga tcacctgttc
     1321 atatttcagc cagtctgttg ccgcctggag gggagtgcgg ttggcctggt tggtgaggtt
     1381 gggctcgttc atgaccagca ggtgctggac ctcgggatgg ctgatgacaa acgccttgac
     1441 cctggtgatg tcggctgcgc tggtgttgcc gccccagagc atcggcacaa agtccatggc
     1501 atagtcggtc tggtaatccg ccggggcgct ggtgctcggg gtgatcgccc agttatacca
     1561 ccagctcacg cc
//
LOCUS       DAOPWU010000553         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_90587_length_1556_cov_2.963358, whole genome shotgun sequence.
ACCESSION   DAOPWU010000553 DAOPWU010000000
VERSION     DAOPWU010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90587_length_1556_cov_2.963358"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PKM23_16165"
     CDS             <1..1140
                     /locus_tag="PKM23_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017714188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNY93059.1"
                     /translation="ILGIRAFGTQLLCEQVIGRALRRQSYELNDDDRFNVEYADVLGI
                     PFDFTARPVAAPPQPPPETYAVKAIRPERDALEIRFPRVQGYRIELPEEKLSAVFNAD
                     SILELTPELVGPSVTRNAGIIGEGVDLTLDHLENLRHSTLLYHLTRRLLYTRWRDPGE
                     APRLHLFGQLKRITRQWLDTCLVCRGGTWPAQLMYQELAEMACERITAGITRSMAGER
                     PVVAVLDPFNSIGSTAHVNFITSKKRRWQTDPRKCHINWVVLDSDWEAEFCRVAEAHP
                     RVRAYVKNHGLGFEVPYRYGSIMRTYLPDFIVLVDDGREELLHLVVEIKGFRGEDAKE
                     KKAAMETCWLPGVNHSGLHGRWAFAEFTEVYEIEKAFGGMIEDHL"
     gene            1153..>1556
                     /locus_tag="PKM23_16170"
     CDS             1153..>1556
                     /locus_tag="PKM23_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY93060.1"
                     /translation="MNSDELIALIASGEDSTRQFKADIRNSDSLASEMAAFANSEGGA
                     LFIGVSDAGAVPGLSQADVVRINQLISNAASQGVRSPLIVQTENVLLPNGRVVIVLGV
                     PKGLARPYFDKNGVIWLKTGADKRRINSREELR"
BASE COUNT          293 a          483 c          478 g          302 t
ORIGIN      
        1 atcctcggca tccgcgcctt cggtacccag ctcctctgcg aacaggtgat cggccgcgcc
       61 ttgcgccgcc aatcctatga gctcaatgac gatgaccgtt tcaacgtcga gtacgccgac
      121 gtccttggca tccccttcga tttcaccgcc aggccggtgg cagcgccgcc gcagccgcca
      181 cccgagacct atgccgtcaa ggcgatacgg ccggaacgcg atgccctcga gatccgtttc
      241 ccgcgcgtgc agggctatcg catcgagctg cccgaggaga agctttcggc ggttttcaac
      301 gcggattcca ttctggaact gacgcccgag ctggtcggcc cctcggttac ccgcaacgcc
      361 ggcatcatcg gcgagggggt ggacctcact ctggatcacc tcgagaatct gcgccattcc
      421 acgctgctct accacctcac ccggcggctg ctctataccc ggtggcgcga cccgggcgag
      481 gcgcccaggc tccacctctt cggtcagctc aagcgcatca cccggcagtg gctcgacacc
      541 tgcctggtct gccgcggcgg tacctggccg gctcagctga tgtatcagga gctggcagaa
      601 atggcctgcg agcgcatcac cgccggcatc acccgctcca tggccggcga gcggccggtc
      661 gtggcggtgc tcgatccctt caattcgatc ggctcgactg cccatgtcaa tttcattacc
      721 tccaaaaagc ggcgctggca gaccgatccg cgcaaatgcc atatcaactg ggtggtgctc
      781 gacagcgatt gggaggccga gttctgccgc gtcgccgagg cccatccgcg ggtgcgggcc
      841 tatgtcaaga accacggcct cggctttgag gtgccctacc gttatggctc gatcatgcgc
      901 acttacctgc ctgatttcat cgtgctggtg gatgacggcc gggaggagct cctgcacctg
      961 gtggtggaga tcaagggatt tcgcggtgag gatgccaagg agaagaaagc ggcgatggag
     1021 acctgctggc tgcccggggt gaaccattcc ggtctccacg gccgctgggc ttttgcagag
     1081 tttacagagg tctacgagat cgaaaaggcg tttggcggga tgatcgaaga tcatctgtag
     1141 aaagaaggtt gtatgaacag tgatgaactc attgcgctta ttgcttcagg cgaagacagc
     1201 accaggcagt tcaaagcgga tattcgcaac agcgattcac tcgcctcgga gatggcggct
     1261 tttgccaaca gcgagggtgg cgccctgttt atcggcgtga gcgatgcagg cgcagtcccc
     1321 ggcttgtcgc aggctgatgt ggttcgcatc aatcagctga tcagcaacgc cgccagccag
     1381 ggcgtacgca gtcctctgat tgtccaaacg gaaaacgtac tgctgccgaa tggccgcgtt
     1441 gtcatcgtcc tgggcgtgcc caagggactc gcgcgcccct attttgacaa gaacggtgtg
     1501 atctggctaa agaccggggc tgacaagcgg cggatcaact cgagagagga actgcg
//
LOCUS       DAOPWU010000554         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_92097_length_1539_cov_3.260108, whole genome shotgun sequence.
ACCESSION   DAOPWU010000554 DAOPWU010000000
VERSION     DAOPWU010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92097_length_1539_cov_3.260108"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1539
                     /locus_tag="PKM23_16175"
     CDS             <1..>1539
                     /locus_tag="PKM23_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY93061.1"
                     /translation="KALWIGTTNYNDPVAGKVYPYKVVHIGPRVFNEMSEWMPSQFKL
                     YGRHDKSTVYVDGTSGGKLDLMDFIDEVNPDLTADRYLYTEANTLIGITEKRKVYQFG
                     QQYHNNYFIQEYTFVNTGIIDSKGTKVNQTLQGVVFFFQHRYSVSKEACVYGLSILPQ
                     SAAWGHNEMNDTMWVSDQAGIEKYLSLFVWKGQHSKAGFDCIGAPDNRSNGDGHLTAP
                     HYAGFMVLHADKSAQDHSHDSAQPTTTQYLGSDVNITSGNDPYNEPKMALEYAAMTAG
                     HPAKSHADGCIASGQAADEYLGTVGGFSHCAGFGPYSIAPGDSVHIVLVEAIAGLGRE
                     ACYTVGAKWLKEEQPYDLPGGGTTTDKNEYKNKWVYTGRDSLIKTFQRAIANYKSGYN
                     IPVPPPAPQRLEVAGGGDRIQLKWTDNAESWPAFAGYKVYRAMHTPDTIFTEIFACGK
                     GTENPQVVNSFDDMTAIRGFDYYYYVVSFDDGSTNVTDANPGGPLHSGIFYAKTINPT
                     NLKR"
BASE COUNT          343 a          514 c          397 g          285 t
ORIGIN      
        1 caaaggccct ctggatcggc acgaccaact ataacgatcc ggtcgccggc aaggtctacc
       61 cctacaaggt ggtccatatc ggcccgcgcg tcttcaacga gatgagcgag tggatgcctt
      121 cgcaattcaa gctttacggc cgccatgaca aatcgacggt ctacgtcgac ggcacctccg
      181 gcggtaaact cgatctcatg gattttattg atgaggtcaa ccccgatctg accgccgacc
      241 gctatctcta taccgaggcc aataccctga tcggcatcac cgaaaagcgc aaggtctatc
      301 agttcggaca gcagtatcac aacaactatt tcatccagga atacaccttt gtcaatacgg
      361 ggattatcga cagcaagggc accaaggtca accagaccct gcaaggggtg gttttcttct
      421 tccagcaccg ctattcggtc tccaaggaag cctgcgtcta cggtctgagc atcctgccgc
      481 agtcggcagc ctggggccac aacgagatga atgataccat gtgggtctcc gaccaggcgg
      541 ggatcgagaa gtacctctcc ctgttcgtct ggaaggggca gcattccaag gctggttttg
      601 actgcatcgg cgccccggac aaccgctcca atggcgacgg ccatctcacc gccccgcact
      661 atgccggctt tatggttctg cacgccgaca agagcgccca ggatcacagc cacgattccg
      721 cccagccgac gacgacccag tacctgggct ccgacgtcaa catcacctcg ggcaatgatc
      781 cctacaacga gcccaagatg gctctcgaat atgcggccat gaccgccggc cacccggcca
      841 aaagccacgc cgacggctgc atcgcctcag gccaggccgc ggatgaatat ctcggcacgg
      901 tcggcggctt ttcccactgc gccggtttcg ggccctactc catagcgccc ggcgacagcg
      961 tgcatatcgt cctggtcgag gccatcgccg gcctgggccg cgaggcctgc tacaccgttg
     1021 gcgccaagtg gctgaaggag gagcagccct acgatcttcc gggcggcggc accaccaccg
     1081 acaagaacga atacaagaac aagtgggtct acaccggtcg tgactccctg atcaagacct
     1141 ttcagcgcgc catcgccaac tataaatccg gttacaatat tccggtgcct cctccggcac
     1201 cccagcgcct cgaggtggcc ggcggcggcg accgcattca gctcaagtgg actgacaatg
     1261 ccgaaagctg gccagctttt gccggctaca aggtctatcg cgccatgcat acccccgata
     1321 ccattttcac agagatcttc gcctgcggca agggcaccga aaatccccag gtggtcaaca
     1381 gcttcgacga catgaccgcc atccgcggct ttgactatta ttattatgtg gtctctttcg
     1441 acgacggcag caccaacgtc accgatgcca atccaggcgg cccactgcac agcggcatct
     1501 tttacgccaa gactatcaac cccaccaacc tcaagcgtc
//
LOCUS       DAOPWU010000555         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1358
            NODE_92744_length_1532_cov_3.125254, whole genome shotgun sequence.
ACCESSION   DAOPWU010000555 DAOPWU010000000
VERSION     DAOPWU010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854563
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:57:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,237
            CDSs (total)                      :: 3,208
            Genes (coding)                    :: 3,187
            CDSs (with protein)               :: 3,187
            Genes (RNA)                       :: 29
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92744_length_1532_cov_3.125254"
                     /isolate="AMR_MDS_1358"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PKM23_16180"
     CDS             <1..921
                     /locus_tag="PKM23_16180"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNY93062.1"
                     /translation="KRTWLVIGLMGMNTLVWAAAPNGTAGFQFLKNHTGARSAGMAGA
                     LVAVAPDMSAISCNPAGIALLSQRTGSFSYLNHLLDFNAGHVGYLQPGVGPGSIAAGI
                     TYMDYGSFDRRDKDNQDLGEFGAGSIAFTGAYAMQPLPNLYAGAGLKYIRASIDDYSS
                     DAVALDGGLLYRIPAQELAIGVSFANFGKVLNPFISGKDPLPTVFRFGFSKKLAHLPL
                     LLSAQLYKYSDEEWHGALGGEFTLTENIFLRLGYDQSGREMHVDQSGDSFAGAALGLG
                     AVWREFQIDYAISSMGALGVLNRFTLTGNF"
     gene            974..>1532
                     /gene="panB"
                     /locus_tag="PKM23_16185"
     CDS             974..>1532
                     /gene="panB"
                     /locus_tag="PKM23_16185"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNY93063.1"
                     /translation="MTQDAQAAVTVPRLHEFKQQGRKIVALTAYDFLFARLLDAAGVD
                     LVLVGDSAAQVAAGRESTLPMTMDEALYHCRMVRRGIRRALLVADMPFMSYQITPEEA
                     LRNAARFFKEAEVDALKLEGGAAIAETIHRITRAGMPVMGHLGLTPQSVRKFGGYGLQ
                     AKDEGAAEQLRVDALALQEAGAFAVT"
BASE COUNT          254 a          522 c          447 g          309 t
ORIGIN      
        1 aagcgcacat ggttggtaat cgggttgatg ggtatgaaca ccctggtttg ggctgccgct
       61 cccaacggca ccgccgggtt ccagttcctg aagaaccata ccggcgcccg ctccgccggc
      121 atggcggggg ccctggtggc ggtcgcaccg gacatgagcg ccatctcctg caatcccgcc
      181 ggcattgccc tcctttcgca gcgcaccggc agcttttcct atctcaacca tctccttgat
      241 ttcaacgccg gccacgtcgg ctacctccag cccggggtcg gaccgggcag tatcgccgct
      301 gggatcacct atatggatta cggatccttc gaccggcggg acaaggataa tcaggatttg
      361 ggcgaattcg gcgccggcag catcgccttc acaggtgcct atgccatgca gcccctgccc
      421 aatctctacg ccggcgccgg actcaaatac atccgcgcct cgattgatga ctactcctcc
      481 gatgcggtgg cgttggatgg cgggctgctt tatcgcatcc ccgcccagga gctggccatc
      541 ggcgtcagct ttgccaattt tggcaaggtg ctgaatccct tcatctccgg caaggatccc
      601 ctgcccacgg tcttccgctt cggcttctcg aagaaactcg cccatctgcc cctgctgctc
      661 agcgcccagc tttataaata ttcggatgaa gagtggcatg gcgccctggg gggagagttc
      721 accctcacag agaacatctt tctgcgcctg ggctacgatc agtccggccg cgagatgcac
      781 gtcgaccagt ccggagacag ttttgccggt gcggcgcttg gactgggcgc tgtctggcgt
      841 gaattccaga tcgactatgc catcagttcg atgggcgctc tgggggttct caaccgcttc
      901 accctgaccg gcaacttttg aacgtctgca cgctcagccg cgccctgctt tcaccttaaa
      961 tctcgggttg atcatgacac aagatgccca ggcagcggtc accgttccac gcctgcatga
     1021 gttcaagcag cagggacgca agatcgtcgc gttgaccgcc tacgattttc tcttcgcccg
     1081 cctgctcgat gccgccggcg tcgatctggt cctggtcggc gattccgccg cccaggtcgc
     1141 ggccggccgc gagtcgaccc tgccgatgac catggatgag gccctttatc actgccgcat
     1201 ggtccgccgc ggcatccggc gcgccctcct ggtcgccgat atgcccttta tgtcctatca
     1261 gattactcct gaagaggccc tgcgcaacgc cgcccgcttt ttcaaggagg cggaggtgga
     1321 tgccctcaag ctcgagggcg gcgcggccat cgccgagacc atccaccgca tcacccgggc
     1381 cggcatgccg gtgatgggcc acctcggcct caccccgcag tcggtgcgca aattcggcgg
     1441 ttacgggctg caggcaaaag acgaaggggc ggccgagcag ctgcgcgtcg atgctctcgc
     1501 cctccaggag gccggggcct tcgccgtcac cc
//
